LOCUS       DASSVX010000001         3333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_483311, whole genome shotgun sequence.
ACCESSION   DASSVX010000001 DASSVX010000000
VERSION     DASSVX010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483311"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1430)
                     /locus_tag="VFO53_00005"
     CDS             complement(207..1430)
                     /locus_tag="VFO53_00005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET8874683.1"
                     /translation="MAPPANVIAHLDMDAFYASVELLRYPELRGKAVVIGGGRRHQPV
                     ETIDPASNAVVRTFATLAGYAGRGVITTATYEARALGLHSAMGMMKAAALAPDAILLP
                     VDFDAYRHHSRRFKEAVRAIAPRIEDRGIDEIYLDLTELARAPCDADATRSTDATAHV
                     RALAQSIKDAVRSATGLSCSIGIARNKLLAKIASDLDKPDGLTLIGDGDLERRIWPLP
                     ARRINGVGPKTAARLAALRINTIGELAHADPAVLTTNFGRAQGEWMHAAAHGRDERPV
                     VTYSEPQSISRETTFERDLSVRRDRALLSSIFTELCDGVGDDLRRKGYAGRTIGIKLR
                     YDDFRTVTRDRTIADATQDAAVIRRVAADCVRRVPLERRIRLFGVRVGGLVRIDTLTV
                     TAREPQPATASLFDY"
     gene            1474..2649
                     /locus_tag="VFO53_00010"
     CDS             1474..2649
                     /locus_tag="VFO53_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HET8874684.1"
                     /translation="MRRSTRRFLTTHTGSLPRPDDLVRAMYAREEGVPVDAEALERRV
                     ASAVTEVVTKQVDAGIDVVDDGEMSKPSYATYVKDRLSGFGGSGNTFVYQDLVGFPNL
                     ARRVFGDPGRSRRKTPACDAPIGIRDPDAARRDVAHLQSALSAVASQEASGDRHRVAD
                     AFLTAASPGVVSLFFRNDHYGSDEAYLAAIADAMRHEYETIANAGLVLQIDCPDLGMG
                     RHIQYAELPLAEFRKKAELHVEALNDALRNIPAEQLRMHLCWGNYEGPHHCDVPLADV
                     IDIVLRAKPAAIVLEAANPRHAHEWQLFETVRLPDGKLLIPGVIESKSNFVEHPELVA
                     QRIGRYANLVGRENVIAGSDCGYGTWVGQAAVDPDVVWAKMAAMAQGARIASGRFFR"
     gene            complement(2817..>3333)
                     /gene="zapD"
                     /locus_tag="VFO53_00015"
     CDS             complement(2817..>3333)
                     /gene="zapD"
                     /locus_tag="VFO53_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HET8874685.1"
                     /translation="QQALAALLTEIEQVNASLLAQAGKVGMHLRENEWLMAIKQRAAI
                     PGGVCEFDLPAYHHWLNKTPPARRQDLAAWIEPFAPIRIGSAIVLRLLRENSRTSRHT
                     ATHGAFQLMMTTTKVAQLLRLTLEHDLPAVPEISANKYALNIRFIGVSGMDRGNVVDQ
                     DIGFELTFCNL"
BASE COUNT          519 a         1116 c         1143 g          555 t
ORIGIN      
        1 ccaaatcgat gcccagcgtc gtaatcttca tagcggtcgc ctcctctcgt gtcagtggat
       61 atgtccacat ttccactgtg gcactctgat gccgcatcgg gaagggggcg accatcccat
      121 tagcgtcttt cgctcgaacg ccccactggg tccccgcctt cgcggggacg acgaaggaag
      181 ccccacgtca cccaattacg taaagctcaa taatcgaaaa gggacgccgt cgccggttgc
      241 ggttcgcgcg cggtcaccgt cagcgtgtcg atgcgcacga gcccgccgac gcgcacgccg
      301 aaaagccgga tgcgccgctc gagcggcacg cgtcgcacgc agtcggccgc cacgcgccgg
      361 atcacggcgg cgtcctgcgt cgcgtccgcg atcgtccgat cgcgcgtcac cgtccggaag
      421 tcgtcgtagc gcagcttgat gccgatcgtg cggcccgcgt agcccttgcg gcgcagatcg
      481 tcgccgacgc catcgcaaag ctcggtgaag atcgacgaga gcagtgcgcg gtcgcgccgc
      541 acggacaggt cgcgctcgaa cgtcgtttcg cggctgatcg actgcggctc gctgtacgtg
      601 accaccggcc gctcgtcgcg gccgtgcgcc gccgcgtgca tccactcgcc ctgtgcgcgc
      661 ccgaagttcg tggtcaaaac cgccgggtcg gcatgcgcga gttcgccgat cgtgtttatg
      721 cgaagcgccg cgagtcgcgc cgcggtcttg ggtccgacgc cgttgatcct tcgcgcgggc
      781 agcggccaga tgcgtcgctc gaggtcgcca tcaccgatca gcgtcaggcc atccggcttg
      841 tcgaggtccg acgcgatctt ggcgaggagc ttgttgcgcg cgatgccgat cgagcacgac
      901 agcccggttg cgctgcgcac ggcgtccttg atcgactgtg cgagcgcacg cacgtgcgcc
      961 gtggcgtcgg tcgagcgcgt cgcatcggcg tcgcacggcg cgcgtgcgag ctcggtcagg
     1021 tcgagataga tttcgtcgat gccgcgatcc tcgatccgtg gcgcgatcgc gcggaccgct
     1081 tccttgaagc gtcgcgaatg atgccggtac gcgtcgaagt cgaccggcag gaggatcgca
     1141 tcgggcgcca gcgcggcggc cttcatcatg cccatcgccg agtgcaatcc gagcgcgcgc
     1201 gcttcgtagg tcgccgtggt gatcacaccg cgcccggcat agcccgcgag cgtggcgaac
     1261 gtgcgcacga cggcgttcga cgccgggtcg atcgtctcga cgggctgatg gcggcgcccg
     1321 ccgccgatga cgacggcctt gccgcgcagc tcgggatagc gcagcagctc gaccgatgcg
     1381 tagaacgcgt ccatgtcgag atgcgcgatg acgttcgccg gcggcgccat cggcttatag
     1441 taaagcttcc tgcatggcaa aggctcgacg acgatgcgca gaagcacgcg acgctttctc
     1501 acgacccata ccggcagcct gccgcggccc gacgatctcg tgcgtgcgat gtatgcgcgc
     1561 gaggaaggcg ttcccgtgga cgcggaagcg ctcgagcgcc gcgtcgcgtc ggcggtgacc
     1621 gaggtggtga cgaagcaggt cgacgcaggc atcgacgtcg tcgacgacgg cgagatgagc
     1681 aagccgagct acgcgaccta cgtgaaggat cggctgagcg gctttggcgg cagcggcaac
     1741 acgttcgtct atcaggatct cgtcggcttt ccgaacctgg cacgccgcgt gttcggcgac
     1801 cctggccgct cgcgacgcaa gacgcccgcc tgcgacgcgc cgatcggcat ccgcgacccc
     1861 gacgcggcgc gaagggacgt cgcgcatctg cagtccgcat tgtctgccgt ggcgtcgcag
     1921 gaagcgtcgg gcgatcgcca ccgcgttgcg gacgcgtttc tcaccgcggc gtcacccggc
     1981 gtcgtatcgc tcttctttcg caacgaccat tacggcagcg acgaggcgta cctggcggcg
     2041 atcgccgatg cgatgcgcca cgaatacgag acgatcgcca acgcgggact cgtgctgcag
     2101 atcgactgtc ccgatctcgg catggggcgc cacatccagt atgcggagct gccgctcgcc
     2161 gaattccgca agaaggccga gttgcacgtc gaggcgctca acgacgcgtt gcgcaacatc
     2221 cccgcggagc aattgcgaat gcacctttgc tggggcaact acgaaggccc gcatcactgc
     2281 gacgtgccgc tcgccgacgt gatcgacatc gtgcttcgcg ccaagccggc ggcgatcgtg
     2341 ctcgaggccg cgaatccccg ccacgcgcac gagtggcagc tgttcgaaac ggtgaggctg
     2401 cccgacggca agctgctgat ccccggcgtc atcgaatcga agtcgaactt cgtcgagcat
     2461 cccgagctcg ttgcgcaacg gatcggccgc tacgcgaatc tggtcggccg cgagaacgtc
     2521 atcgccggga gcgattgcgg ctacggaacg tgggtcggtc aggcggccgt cgatcccgac
     2581 gtggtgtggg cgaagatggc ggcgatggcg cagggggcgc gcatcgcctc ggggcggttc
     2641 tttcgctagc ggcgcgttcc ggccgttcgc tggcgtcgcg cgccgccgtt cattttgcct
     2701 tcaggcaccg ggccgcggcg caggtgaagc ggcccgacgc ggccctcgcc gagctcgccg
     2761 aagccgcgcg ctacgacgat cgcgtgcgcg cgcttgccac cgattagggc cgtcgttcag
     2821 agattgcaga acgtgagctc gaagccgatg tcctgatcga cgacgttgcc gcggtccatg
     2881 ccggacacgc cgatgaagcg gatgttgagc gcgtacttgt tcgcgctgat ctcgggaacc
     2941 gccggcaggt cgtgctcgag cgtgagccgc aggagctgcg cgaccttggt cgtggtcatc
     3001 atcagttgga acgcgccgtg cgtcgccgtg tggcggctcg tgcggctgtt ctcgcgcaaa
     3061 agccgcagca cgatcgcgga gccgatgcgg atcggcgcga acggctcgat ccaggccgcg
     3121 aggtcctgcc gtcgtgccgg cggcgtcttg ttgagccagt ggtggtaggc gggcaggtcg
     3181 aattcgcaga cgccgccggg aatggcggcg cgctgcttga tcgccatcag ccactcgttc
     3241 tcgcgcagat gcatgcccac cttgcccgcc tgggcgagca ggcttgcgtt cacctgctcg
     3301 atctcggtca gcagcgccgc gagcgcctgc tgg
//
LOCUS       DASSVX010000002        10128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1616526, whole genome shotgun sequence.
ACCESSION   DASSVX010000002 DASSVX010000000
VERSION     DASSVX010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 10128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10128
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616526"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VFO53_00020"
     CDS             complement(<1..843)
                     /locus_tag="VFO53_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874686.1"
                     /translation="MRFAAKAAVVVLGTFFALLLGIRVVVYPQLEAHRVDIARWLGER
                     IGQPVEIDDIVTGWDGWNPRLSIRGFRVHERTGTATLLDLPRVDLLIAWTSLPRLDLR
                     LKELTIDSPRLAVRRDSQGRLHLAGLEMTGDDSADDSPFANWVLRQPQVIVRDALVAW
                     NDELRHAPQLLLDHVQFRLEQRFGRHEAGLTGVPPPEIAGPIDMRADLTGMSRRDFSH
                     VRGRLYFRLDYADVAAWREWLPLPFALESGRGAVRMWADFAGGQANDVTADLELADVR
                     ATLGH"
     gene            971..3685
                     /gene="glnE"
                     /locus_tag="VFO53_00025"
     CDS             971..3685
                     /gene="glnE"
                     /locus_tag="VFO53_00025"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HET8874687.1"
                     /translation="MDLDRALAYSRYAQHALNAHPALRDELAATIDAPFDWTNAFAAV
                     DAALAGDDAPWLAAALRVLRRRAFVHTLARDLTGRAHFAEVAQAMTTLAERSLAAAVE
                     LHARALAATFGVPRGDASGAPQRLIVVAMGKLGGGELNVSSDIDIVFVYPEEGETDGA
                     KRISNREFFDRLAPRIVAALDQVDANGYVFRVDTRLRPYGESGPPTVSFAALERYLVT
                     QGRAWERYAWLKARPVTGERHDELAALVSPFVYRKYLDYDAYEGLRAIHRQIRDQERR
                     YGDDIKLGAGGIREMEFVIQALQIVRGGREPALRARGTLPALAALAQRMIIAPHAAGV
                     LRDAYLFLRKLEHRLQYRDDRQTQRLPSGDERALVAETLDFGSTEAFERVLAAHRADI
                     AAQFNAVFDAPADAGPDADRSQRHAALWDDPRPTPEALDELAQAGFPDAAAVVQSLSR
                     MRASRRYLQLPALSRDRFDRLVPRLVGVASEHRGPGASAAVVLSRLLGLLEAISGRSA
                     YLALLTEHPPLLPRLAQLMGASAWAADYLTRRPLVLDELLDARVLLAEPDWTQWREEL
                     DRVLAPHAHDAEAAMDALRHFQHAQVFRLLTQDLAGRLSVERLADHLSALADAILDAT
                     LRCCWRQLRGEDAKPPRFAVVAYGKLGGKELGYVSDLDLVFLYDDDPALPDADDAFVH
                     HARLAQRINTWLSAATGAGRLYETDLRLRPDGAKGLLVSGMRAFERYQREQAWTWEHQ
                     ALTRARFVAGDGTVGAAFETIRDGLLRVPRDRAQLATDIAEMRERMAAGHTNATTLFD
                     LKHDRGGMVDIEFAVQFIVLAHAHVHRDLTRNAGNIALLSLAADAGLLPRALALEAAD
                     AYRAYRRLQHQVRLTGAAHARVDPAPQTARRACVDALWSAVFGAPRNA"
     gene            3733..4659
                     /locus_tag="VFO53_00030"
     CDS             3733..4659
                     /locus_tag="VFO53_00030"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET8874688.1"
                     /translation="MSMADRDGWIWYDGKMVPWRDATTHVLTHTLHYGMGVFEGVRCY
                     ATDKGPALFRLRDHTNRLFRSARIFAMRIPFTPDEIFEAQRACVRENKLAACYIRPIA
                     FYGSVAMGVAARSNPVRVAIAAWPWGAYLGAEGLEKGIRVKTSSFTHHHPNVTMCGAK
                     AVSNYSVSILANQEAAHDGYEEAMLLDPQGYICQGSGENVFIVRDGKLHTPDLAGGAL
                     AGITRATVMEFAAELGIPVLERRITRDEVYVADEAFFTGTAAEITPIREYDHRPVGDG
                     ARGPITTRLQTMFFDTVNGRNPARAEWLSYAG"
     gene            4656..4847
                     /locus_tag="VFO53_00035"
     CDS             4656..4847
                     /locus_tag="VFO53_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HET8874689.1"
                     /translation="MMAADLPVVEVRAIDLPVFCPNPGMPLWSSHPRVFLDVAGAGEA
                     MCPYCGTRYRLVGEAKAGH"
     gene            4799..5941
                     /gene="waaF"
                     /locus_tag="VFO53_00040"
     CDS             4799..5941
                     /gene="waaF"
                     /locus_tag="VFO53_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HET8874690.1"
                     /translation="MRNALSPGRRSEGRTLSGASTHAGPPSVAGRHAGDDRVLLIAPS
                     WVGDAILSEPLIAALRRNGVTKAIDVVAPSWCGPVYARMRGVGRILDAPAAHGEFALR
                     ARYAFGLALREHRYTHAYVLPNTWKSALVPCFARIPVRVGYTGEARYGLLTDARKLDR
                     KTLPQLVSRYAALGEPRHASASGAPAPRLVPNAANLAAAMQALALSRAHPVAILCPGA
                     EFGPAKRWPARHFADLAQRLANDDHQVWIVGSPNDRAVADALLAALPAGTPRVIDLTG
                     RTDLGTAIDLMSVASLVVSNDSGLMHAAAAVNAPLVALFGSSSPAYTPPASPAARIAK
                     IDIECSPCFKRECPLGHFRCMRELSPELVYNLARETAATNLPSFRR"
     gene            5946..6377
                     /locus_tag="VFO53_00045"
     CDS             5946..6377
                     /locus_tag="VFO53_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET8874691.1"
                     /translation="MPTARTPPIYTSPQDAAQAFYQAFEARDIDAMMATWAEDEDIVC
                     VHPGGPRHVGYDAVRHSFEQLFAGDTRLTFRLDQAVSIDTVGLAVQSVVEHLRAGEEG
                     PRGAALATNVLMRTPYGWRMVCHHASPSPPVAESPQTGPLH"
     gene            complement(6391..7854)
                     /gene="tldD"
                     /locus_tag="VFO53_00050"
     CDS             complement(6391..7854)
                     /gene="tldD"
                     /locus_tag="VFO53_00050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HET8874692.1"
                     /translation="MTEATLAPISPALLADADRLLLAPAGLDLDKVSGVLASIHAHDI
                     DFADFYFQHSCFESWSLEEGIVKAGTFNIDRGVGVRAVSGDKQAFAYSDDISLAALEE
                     AAAAVRAIGRQGQSASAPLERTGEARLLYPTADPVLSLGDAAKVDVLMRLERIARALD
                     PRVVQVMASLAGEHETVLVARSDGLLAADVRPLVRMSITVIVEEHGRREQGYAGGGAR
                     LDYGYFTEAELASFARRAVDQALVNLSARDAPAGNMTVVLGNGWPGILLHEAVGHGLE
                     GDFNRKGTSAFSGRIGQRVAAPGVTVVDDGTLGERRGSLNLDDEGHPTQRTVLIEDGI
                     LRGYMQDRLNAGLMKSGATGNGRRESFAHLPMPRMTNTIMLPGAYDSAEIIASVKKGL
                     YAANFGGGQVDITSGKFVFSAAEAYMIEDGRITYAVKGATLIGNGPDALTRVTMVGND
                     LELDAGIGTCGKDGQSVPVGVGQPTLKIEGLTVGGTV"
     gene            complement(8043..8468)
                     /gene="mscL"
                     /locus_tag="VFO53_00055"
     CDS             complement(8043..8468)
                     /gene="mscL"
                     /locus_tag="VFO53_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET8874693.1"
                     /translation="MSFTSEFREFAVKGSVIDLAVGIIIGAAFGKIVDSLVKDIIMPV
                     VGAIFGGLDFSNWFIMLKSPPAGYSGPMTYDALTKAGVPLFAYGSFITAVINFLILAL
                     IIFIMVKQINRLKRQTPPAPPAPPEEVVLLREIRDAVRR"
     gene            complement(8496..8834)
                     /locus_tag="VFO53_00060"
     CDS             complement(8496..8834)
                     /locus_tag="VFO53_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET8874694.1"
                     /translation="MKKIEAIIKPFKLDEVREALSEVGVSGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVELILADSMVDSAIEAIVRAARTGKIGDGKIFVTAIEQVVRI
                     RTGESGDAAI"
     gene            complement(8892..>10128)
                     /locus_tag="VFO53_00065"
     CDS             complement(8892..>10128)
                     /locus_tag="VFO53_00065"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET8874695.1"
                     /translation="VFDVDGVRCGIVICEDCWFAEPAAQARDAGAQLIVVPNGSPYHT
                     QQQSARRAQLEARVRETGLPFVYVNRFGGQDELVFDGASFVMDARGAIAQQLPAWHEA
                     IAITEFDGAIPRTVRGDIDCALEPNVYQALVVGVRDYVSKNRFPGVLLGLSGGVDSAL
                     TLAVAVDALGPARVRAVMLPSQYSASISLEDAREMAGIAGVRYDEIAIGPMFDAFLGS
                     LQAEFAGLPVDAAEENIQARIRGTLLMALSNKFGAIVLTTGNKSEMAVGYATLYGDMA
                     GGFAVLKDISKTLVYRLCNYRNRLGRVIPERIITRPPSAELRPDQVDQDSLPPYDVLD
                     AILEAYVERNLAPAAIIAEGLPADAVQKVVRLIKINEYKRRQAAVGIRITPRGFGKDW
                     RYPITSAWNEWERVGVARP"
BASE COUNT         1635 a         3472 c         3387 g         1634 t
ORIGIN      
        1 gtggccgagc gtcgcgcgca cgtcggcgag ctcgaggtcc gcggtgacgt cgttcgcctg
       61 gccgcccgcg aaatcggccc acatccgcac ggcgccgcga ccgctttcga gcgcgaacgg
      121 caacggcagc cattcgcgcc acgcggcgac gtcggcgtag tcgaggcgaa agtaaagcct
      181 gccccgcacg tgcgagaagt cgcggcgcga catgccggtg aggtcggcgc gcatgtcgat
      241 cggtccggcg atctccggcg gcggcacacc ggtgaggccg gcctcgtggc ggccgaatcg
      301 ctgctcgagc cggaactgaa cgtgatcgag caatagctgc ggcgcgtgac gcaactcgtc
      361 gttccaggcg acgagcgcgt cgcggacgat cacctgcggc tggcgcaaca cccagttggc
      421 gaacggcgaa tcatcggcgc tgtcgtcgcc ggtcatttca agaccggcca gatgcagccg
      481 tccctgcgag tcgcgccgca cggcgaggcg cggcgagtcg atcgtcagct ccttgagacg
      541 caggtcgaga cgcggcagcg acgtccacgc gatcaaaaga tcgacacgcg gcaagtccag
      601 cagggtcgcc gtgccggtgc gctcgtgcac gcgaaagccg cggatcgaaa ggcgcggatt
      661 ccagccgtcc caacccgtga cgatgtcgtc gatctcgacc ggctggccga tccgttcgcc
      721 gagccagcgc gcgatgtcga cgcgatgcgc ttcgagctgc ggatagacga cgacccggat
      781 gccgagcagc agcgcgaaaa aggtgccgag gacgacgact gccgccttcg cggcgaaccg
      841 cacgacgcgg tcgacccggg atgacgccga cgcggtcgaa ggcataatga acctgaattg
      901 aagcgcaccg attgtagcgg atgaaccggg tgccgggtgc cggccggcct ttcgcgaaag
      961 ccaccccgcc atggatctcg accgcgcgct cgcctacagc cgctatgcgc aacatgcgct
     1021 gaacgcgcac cctgcgctgc gcgacgagct cgcggcaacc atcgacgcac cgttcgactg
     1081 gacgaacgcc ttcgccgcgg tcgacgcggc gcttgccggc gacgacgcac cgtggctcgc
     1141 cgctgcgctg cgtgtgctgc ggcggcgcgc attcgttcat acgctcgccc gcgacctcac
     1201 cggaagggcg cacttcgccg aggtcgcgca ggcgatgacc acgctcgcgg agcgctcgct
     1261 cgcggctgcc gtcgagctcc atgcccgcgc gctggcggcc acattcggcg tgccgcgcgg
     1321 cgacgcgtcg ggtgcgccgc aacggctcat cgtcgtcgca atgggcaagc tcggcggtgg
     1381 tgagctcaac gtgtcgtcgg acatcgacat cgtcttcgtc tacccggaag aaggcgagac
     1441 cgacggtgcg aagcgcatct ccaaccgtga gttcttcgat cggctggcac cgcgcatcgt
     1501 cgccgcactc gaccaggtgg acgcgaacgg ctacgtgttt cgcgtcgaca cgcggttgcg
     1561 cccgtatggc gagagcggac cgccgaccgt gtcgttcgca gcgctcgagc gttacctggt
     1621 gacgcagggc cgcgcatggg agcgctatgc gtggctcaag gcgcgcccgg tcaccggcga
     1681 gcgccacgac gagcttgccg cactcgttag cccgttcgtg tatcggaaat acctcgacta
     1741 cgacgcatac gagggcctgc gcgcaatcca ccgccagata cgcgatcagg aacggcgtta
     1801 cggcgacgac atcaagcttg gcgcgggtgg cattcgcgaa atggaattcg tcatccaggc
     1861 gctgcagatc gtgcgcgggg gtcgcgagcc ggcactgcgg gcgcgcggca cgctccccgc
     1921 actcgcagcc ctcgcccagc gcatgatcat cgcgccgcac gctgccggcg tgttgcgcga
     1981 cgcgtatctg ttcctgcgca agctcgaaca ccggctgcag tatcgcgacg atcgccagac
     2041 gcagcgcctg ccttcgggcg acgagcgcgc gctcgtcgcc gagacgctcg atttcgggtc
     2101 gacggaagcg ttcgagcgcg tgctggctgc acaccgggca gacatcgcgg cgcagttcaa
     2161 cgccgtgttc gacgcacccg ccgacgcggg acccgacgcc gaccgatcgc agcgccacgc
     2221 cgcgctctgg gatgatccgc ggccgacgcc ggaggcgctc gacgagctcg cgcaagccgg
     2281 ctttcccgac gcggccgcgg tcgtgcaatc gctttcgcgg atgcgcgcga gccggcgcta
     2341 tctgcaactg cctgcactgt cgcgcgaccg gttcgatcgc ctcgtgccgc ggctcgtcgg
     2401 cgttgcgtcc gagcatcgcg gcccgggcgc gtcggcggcg gtggtgcttt cgcggctcct
     2461 ggggctgctc gaagccatca gcggacgcag cgcctatctg gcgttgctca ccgagcatcc
     2521 gccgctgctg ccgcgacttg cgcagctgat gggcgcgtcg gcgtgggcgg ccgattacct
     2581 gacgcgccgg ccgctcgtgc tcgacgagct gctcgatgcc cgcgtgctgc tcgcggagcc
     2641 cgactggacg caatggcgcg aggagctcga tcgcgtgctt gcaccgcacg cgcacgacgc
     2701 cgaggcggcg atggacgcgc tgcgccattt ccagcacgca caggtgtttc gactgctgac
     2761 gcaggacctc gcgggtcggt tgagcgtcga gcggctggcc gatcatttgt cggcacttgc
     2821 cgatgcgatc ctcgacgcga cgctgcgctg ctgctggcgg cagttgcggg gcgaggatgc
     2881 gaagccgccc cgcttcgcgg tggttgccta cggcaagctc ggcggcaagg agctcggcta
     2941 tgtgtcggat ctcgacctcg tgttcctcta cgacgacgat ccggccctcc ccgacgccga
     3001 cgacgcgttc gtgcaccacg cgcgactcgc gcaacgcatc aacacgtggc tttcggcggc
     3061 caccggcgcc ggacggctgt acgagaccga tctgcggctt cgccccgacg gggcgaaggg
     3121 cctgctcgtt tccggcatgc gcgccttcga gcgctatcag cgcgagcagg cgtggacctg
     3181 ggagcaccag gcactgacgc gcgcacgctt cgtcgccggc gacggcacgg ttggcgcagc
     3241 attcgagacc atccgcgacg gcttgttgcg cgtgccgcgc gatcgcgcgc aactcgcgac
     3301 cgatatcgcg gaaatgcgcg agcggatggc cgccggccat acgaatgcga cgacgctgtt
     3361 cgacctcaag cacgatcgcg gcggcatggt cgacatcgag ttcgccgtgc agttcatcgt
     3421 gctcgcgcac gcgcatgtcc atcgcgacct cacgcgcaac gccgggaaca tcgcgctcct
     3481 gtcgctcgcc gccgacgcgg gcctgctgcc gcgcgcactg gcgctcgagg cggccgacgc
     3541 ataccgggcg tatcggcggc tgcagcacca ggttcgcctc accggtgccg cacatgcgcg
     3601 tgtcgacccg gccccgcaga cggcgcgccg ggcgtgtgtc gacgcgctgt ggagcgccgt
     3661 cttcggcgca ccgcggaacg cgtgacgccg gcctgctaaa atcgtctgtc ttcagcggga
     3721 aggacgcacg ccatgtcgat ggccgatcgc gacggatgga tctggtacga cggaaagatg
     3781 gtgccgtggc gggatgcgac cacgcacgtg ctcacgcaca cgctgcatta cggcatgggc
     3841 gtgttcgagg gcgtgcgctg ctacgcgacc gacaaggggc cggcgctctt tcgtctgcgc
     3901 gaccacacga accggctctt ccgctcggcg cgcatattcg cgatgcggat tccgttcacg
     3961 ccggacgaga tcttcgaagc gcagcgcgcc tgcgtgcgcg agaacaagct cgcggcatgc
     4021 tacatccggc cgatcgcgtt ctacggatcc gttgcgatgg gcgtcgcggc gcggtcgaat
     4081 cccgtgcgcg tggcgattgc cgcgtggcca tggggcgctt acctcggtgc cgaagggctc
     4141 gagaagggca tccgcgtcaa gacgtcgtcg ttcacgcatc accatccgaa cgtcacgatg
     4201 tgcggcgcga aggcggtatc gaactattcg gtgtcgatcc tcgcgaacca ggaggcggcg
     4261 cacgacggtt acgaggaggc catgctgctc gacccgcagg gctacatctg ccagggatcg
     4321 ggcgaaaacg tgttcatcgt gcgcgacggc aaactgcata cgcccgatct ggccggcggc
     4381 gcactggccg gcatcacgcg cgccacggtg atggagttcg ccgccgagct cggcatcccg
     4441 gtcctcgagc ggcgcatcac acgcgacgag gtgtacgtcg ccgacgaggc gttcttcacc
     4501 ggcacggcgg ccgagatcac gccgatccgc gaatacgacc atcgcccggt cggcgatgga
     4561 gcgcgcggac cgatcaccac gcgcctgcag acgatgttct tcgacacggt caacggccgc
     4621 aatcccgcgc gggccgagtg gctgtcgtac gcaggatgat ggcggccgat ctgcccgtgg
     4681 tcgaggttcg cgcgatcgac ctgccggtgt tctgtcccaa tccgggcatg ccgctgtgga
     4741 gctcgcaccc gcgcgtgttc ctcgacgtcg cgggcgccgg cgaagcgatg tgcccgtatt
     4801 gcggaacgcg ctatcgcctg gtcggcgaag cgaaggccgg acactgagcg gcgcctcgac
     4861 gcacgcaggt ccgccatccg ttgccggccg ccacgccggc gatgatcgtg tcctgctgat
     4921 cgcgccgtcg tgggtcggcg atgcgatcct gtccgagccg ctcatcgccg cgcttcgccg
     4981 caacggcgtc acgaaagcga tcgacgtggt ggcgccgtcc tggtgcggac ccgtttatgc
     5041 acgcatgcgc ggcgttggcc gcatcctcga cgcacccgca gcgcacggcg agttcgcatt
     5101 gcgtgcgcgc tacgcattcg ggcttgcgct tcgtgagcac cgctacacgc acgcatacgt
     5161 gctgccgaac acgtggaaat cggcgctcgt gccgtgcttc gcgcggattc ccgttcgggt
     5221 cggctacacc ggtgaagcgc gctacggttt gctgaccgac gcacgcaagc tcgaccgaaa
     5281 gacgctgccg cagctcgtgt cgcgctatgc cgcactgggc gaacctcggc acgccagtgc
     5341 gtcgggggcg cccgcgccgc gactcgtgcc gaacgcggcg aatctcgctg cagcgatgca
     5401 ggcgctcgcc ttgtctcgcg cacacccggt ggcgatactg tgtcccggcg ccgagttcgg
     5461 gccggcgaag cgctggcccg cgcgccactt cgcagacctg gcgcagcggc ttgcaaacga
     5521 cgatcatcaa gtatggatcg tcggctcgcc aaacgatcgc gcagtcgccg acgcgctgct
     5581 cgcggcgctg ccggcaggca cgccgcgggt gatcgatctc accggacgca ccgatctcgg
     5641 cacggcgatc gatctcatgt cggtggcctc gctcgtcgtc agcaacgatt ccggcctgat
     5701 gcacgcggcg gcggcggtga atgcgccgct ggtggcactg ttcggatcgt cgtcgccggc
     5761 ctatacgccg cccgcgtcac cggcggcccg catcgcgaag atcgatatcg aatgcagccc
     5821 ctgcttcaaa cgcgaatgtc cgctgggcca tttccggtgc atgcgcgaat tgtcgcccga
     5881 gttggtctac aatctggcgc gcgaaactgc tgcaacgaat ctcccctcgt tccgtcgctg
     5941 acgccatgcc cacggcacgc acgccgccga tctacacttc gccgcaggac gccgcccagg
     6001 cgttctacca ggccttcgag gcgcgcgaca tcgatgcgat gatggcgacg tgggccgagg
     6061 atgaagacat cgtgtgcgtg caccccggtg ggcctcgtca cgtcggttac gacgccgtcc
     6121 gccacagctt cgagcagctt tttgccggcg acacgaggct cacgttcagg ctcgaccagg
     6181 ccgtgtcgat cgacaccgtg ggtctcgccg tgcaaagcgt ggtcgagcac ctgcgtgcgg
     6241 gcgaagaagg tccccgcggc gcggcgctcg cgaccaacgt gctgatgcga acgccgtacg
     6301 gctggcgcat ggtgtgccat catgcctcgc cgtcgccgcc ggtcgcggag tcaccgcaga
     6361 ccggcccgct gcattagcgg acgggcggcc ctagacggtg ccgccgacgg tgaggccttc
     6421 gatcttgagc gtgggctgtc cgacgcctac cggcacgctt tgtccatcct tgccgcaggt
     6481 gccgatgccg gcgtcgagct cgaggtcgtt gcctaccatc gtcacgcgcg tcagcgcatc
     6541 gggcccattg ccgatcagcg tcgcgccctt cacggcatag gtgatccttc cgtcctcgat
     6601 catgtacgcc tcggccgcgg agaacacgaa cttgccgctc gtgatgtcga cctggccgcc
     6661 gccgaaatta gcggcataaa ggcccttctt caccgacgcg atgatctccg ccgagtcgta
     6721 ggcgccaggc agcatgatcg tgttggtcat gcgcggcatc ggcaggtgcg caaacgattc
     6781 gcgccggccg ttccccgtgg cgccgctttt catcaatccc gcgttcagcc gatcctgcat
     6841 gtacccgcgc aggatgccgt cctcgatcag caccgtgcgc tgcgtgggat ggccttcgtc
     6901 gtcgagattg agcgatccgc gacgctcgcc gagcgttccg tcgtcgacga cggtgacgcc
     6961 cggcgcggca acgcgctgtc cgatgcgtcc gctgaacgcg ctcgtgccct tgcggttgaa
     7021 atcgccctcg agtccatggc cgaccgcctc gtgcagcagg atccccggcc aaccgttgcc
     7081 gagcacgacg gtcatgttgc cggccggtgc atcgcgcgcc gacaggttga cgagcgcctg
     7141 gtcgacggcg cgcctggcaa acgacgcaag ctccgcttcc gtgaagtagc catagtcgag
     7201 acgcgcgcca ccgccggcgt aaccctgctc gcgccggccg tgctcctcga cgatcacggt
     7261 gatcgacatg cgcacgagcg gccgcacgtc ggcggcgagc agtccgtcgc tgcgcgcgac
     7321 cagcaccgtc tcgtgctcgc cggcgagcga cgccatcacc tgcacgacgc gcggatcgag
     7381 tgcgcgcgca atgcgctcga gtcgcatcaa cacgtcgacc ttggccgcgt cgccgaggct
     7441 cagcacggga tccgccgtgg ggtaaagaag acgcgcctcg ccggtgcgct cgagcggggc
     7501 cgacgccgat tgaccctggc ggccgatggc gcgcacggcg gccgctgcct cctcgagtgc
     7561 agcaagcgag atgtcgtcgg aataggcaaa cgcctgcttg tcaccgctta ccgcgcgcac
     7621 gccgacgccg cggtcgatgt tgaacgtgcc ggccttgacg atgccttcct cgaggctcca
     7681 gctttcgaag caggaatgct ggaagtagaa atcggcgaag tcgatgtcgt gcgcgtggat
     7741 cgacgcgagg acgccggaca ccttgtcgag gtcgaggccc gcgggtgcga ggagcagccg
     7801 atcggcatcg gcgagcagcg cgggggagat cggggcgagc gtggcttctg tcaccggaat
     7861 ccttgagagt caacaaaacg acgcagcttg agcgcgccgg gcgaatacct gtcgatgatg
     7921 ggggttgcgg cgaggcgctt caacatgcgg gcccgatttg ccctcggcca ttgccgcgct
     7981 gtgcaatccg gtggcgcaaa cccggtgccc gatcggcacc gggtccgcat aacgagcggc
     8041 cgctagcgcc gcacggcgtc gcggatttcg cgcagcagca cgacctcttc cggaggcgcg
     8101 ggcggcgccg gcggcgtctg ccgcttcagc cgattgatct gcttcaccat gatgaagatg
     8161 atcagcgcga ggatcaggaa gttgatcacc gcggtgatga agctgccgta cgcgaacagc
     8221 ggcacgcccg ccttggtcaa cgcgtcgtac gtcattggtc cggagtaacc cgcgggcggg
     8281 cttttcagca tgatgaacca gttcgagaag tcgaggccgc cgaagatcgc gccgaccacg
     8341 ggcatgatga tgtccttcac gagcgagtcg acgattttcc cgaatgccgc gccgatgatg
     8401 atgcccaccg cgagatcgat gacactgccc ttgacagcga attcacgaaa ttcactcgta
     8461 aacgacatgc tcgcctccgt tggataggtg tccgttcaga ttgccgcgtc gcccgattcc
     8521 ccggtgcgga tacgcacgac ttgctcgatc gccgtgacga agatcttgcc gtcgccgatc
     8581 ttgccggttc gcgcggccct cacgatcgcc tcgatcgcgg aatcgaccat cgaatcggcg
     8641 agaataagct cgaccttcac cttcggcagg aaatcgacga catactccgc gccgcggtac
     8701 aactccgtat ggcccttctg gcggccgaat cccttcacct cggtcaccgt gagaccggaa
     8761 acaccgacct cggaaagcgc ctcgcgcact tcgtcgagct tgaagggctt gatgatcgct
     8821 tcgatctttt tcatcgtcat gggtcctcgt gcggccggcc gcggcgggaa gaatacacga
     8881 acggtacgcc gctacgggcg cgcaacgccg acgcgctccc actcgttcca cgccgacgtg
     8941 atcgggtagc gccagtcctt gccgaagccg cgcggcgtga tgcgaatgcc gaccgcggcc
     9001 tggcggcgct tgtattcgtt gatcttgatg agccgcacga ccttctgcac cgcatcggcg
     9061 ggaagccctt ccgcaatgat tgccgcaggg gcgagattgc gctcgacgta cgcctccagg
     9121 atggcgtcga gcacgtcgta gggcggcagc gaatcctggt cgacctggtc gggacgcagt
     9181 tcggcggagg gtggacgggt gatgatccgc tcgggaatca cgcgaccgag gcgattccgg
     9241 taattgcaga ggcggtagac gagcgtcttg ctgatgtcct tcagcaccgc gaagccaccc
     9301 gccatgtcac cgtacagcgt tgcatagcct actgccattt ccgacttgtt gccggtggtg
     9361 agcacgatgg ccccgaactt gttcgaaagc gccatcaaca gcgtgccgcg aatgcgcgcc
     9421 tggatgttct cctccgccgc gtcgacggga agaccggcga attcggcttg caatgacccg
     9481 aggaacgcat cgaacatcgg gccgatcgca atctcgtcgt agcgcacacc ggcgatgcca
     9541 gccatttcgc gcgcatcctc gaggctgatc gatgcgctgt attgcgacgg cagcatgacc
     9601 gcacgcacac gagcgggccc gagcgcatcg acggccaccg cgagcgtcaa tgcggaatcg
     9661 accccgccgg agagacccag cagcacgccg ggaaagcggt tcttcgatac atagtcgcga
     9721 acacccacga caagcgcctg gtacacgttc ggctcgagcg cgcaatcgat gtcgccccgg
     9781 accgttcgcg ggatcgcgcc gtcgaattcg gtgatcgcaa tggcttcatg ccacgcgggg
     9841 agctgctgcg cgatcgcgcc gcgcgcgtcc atcacgaacg acgcgccgtc gaacacgagc
     9901 tcgtcctgtc cgccgaaccg gttcacgtac acgaacggaa ggccggtttc gcgcacgcgg
     9961 gcttcgagtt gcgcgcggcg cgcggactgc tgctgcgtat ggtaaggcga cccgttcggc
    10021 acgacgatca actgcgcgcc cgcgtcacgc gcctgcgccg ccggctccgc gaaccagcaa
    10081 tcctcgcaga tgacgatgcc gcagcgcacg ccgtcgacgt cgaacacg
//
LOCUS       DASSVX010000003        11410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_200008, whole genome shotgun sequence.
ACCESSION   DASSVX010000003 DASSVX010000000
VERSION     DASSVX010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 11410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11410
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200008"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VFO53_00070"
     CDS             complement(<1..552)
                     /locus_tag="VFO53_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8874696.1"
                     /translation="MAKALDGVKILDFTHVQSGPTCTQLLAWFGADVIKVERAGEGDI
                     TRGQLRDIPDVDSLYFTMLNHNKRSITIDAKNPKGKEILERLVRECDVLVENFAPGAL
                     DRMGLTWEHIQELNPKMIVASIKGFGPGPYADCKVYENVAQCAGGSASTTGFDDGPPL
                     VTGAQIGDSGTGLHLALGIVCALY"
     gene            complement(681..2477)
                     /gene="oxc"
                     /locus_tag="VFO53_00075"
     CDS             complement(681..2477)
                     /gene="oxc"
                     /locus_tag="VFO53_00075"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HET8874697.1"
                     /translation="MESVTATRKGAASAATESGLATSGGQANVQSRPEIDGFHLLIDA
                     LKLNGIDTIFGLPGIPITDLTRKAQAAGLRVISFRHEQNAGYAASIAGFMTQKPGICL
                     TVSAPGFLNGLTALANATTNCFPMILISGSSEREVVDLQQGDYEEMDQLAIAKPLAKA
                     AFRVLHADDIGVGVARAIRAAVSGRPGGVYLDLPAKLFPQAMDAEAGRQSLIKVVDPA
                     PRQIPSQDAVTRALDLLKGAAKPLIILGKGAAYAQADADIRALIEKTGIPYLPMSMAK
                     GLLPDTHAQSAAAARSYVLPEADVVMLIGARLNWLLSHGKGKTWGGKSAKAWGGQKFI
                     QVDISPTEADSNVRIDAPVVGDIGSCVSAMLAAIDASWPKPPAAWLDAIAERKSRNIA
                     KMAETLAKNSTPMNFHGALNVVRDIVKANPGAILVNEGANALDFTRSIVDMYEPRKRL
                     DVGTWGVMGIGMGFSVAAAVVSGEQVIAIEGDSAFGFSGMEVETICRYDLPVCVVILN
                     NNGVYRGDETNWAGKSDPSPLVFVKDARYEKLMEAFGGVGVHVTTPGELRKALEEAIR
                     SRKPTLVNAVIDERAGTESGRITGLNPTAKKT"
     gene            2845..3525
                     /locus_tag="VFO53_00080"
     CDS             2845..3525
                     /locus_tag="VFO53_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8874698.1"
                     /translation="MPLALRPLSANVSFRDQAYAALKQAITEADIYSHPHELRLDERQ
                     LSRSLGVSRTPIREAMTVLEQEGFLRTVPRRGIFIVRKTKRQIIEMIEMWAALESMAA
                     RLATLNASDAEIATLRTMFDEFRNSTPAEHIDEYSHANIAFHQAIIRLSGSHLMGKTI
                     ENLFIHVRAMRRLTISQSDRAARSIVDHMRIIEALERRDTELAERLVRDHSLDLAAFV
                     EKHCDFLE"
     gene            3531..5261
                     /locus_tag="VFO53_00085"
     CDS             3531..5261
                     /locus_tag="VFO53_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET8874699.1"
                     /translation="MTDPVVVDTSKLRGGRGVKARPKGRHVDAQALADVQRLLGDMPR
                     ARSLLIECLHRIQDARGYLPASYLVALAQEMKLAMTEVYEVATFYHHFDVVDADDAPP
                     PPITVRVCETLSCKMAGADALRATLVAHGDANVRVLGAPCIGRCELAPAAVVGRNPIG
                     HATPERILDAIRTRALEPAPAPYVDYAAYRRDGGYRTLVDCVGGQRSVEAVIAEMETS
                     SLRGLGGAGFPAGRKWKIVRAEPAPRLMAVNIDEGEPGTFKDRYYLERDPHRFLDGML
                     IAAWAVGIDAIYVYLRDEYAGCRAILQREIDALVADPPCPLPRIELRRGAGAYICGEE
                     SAMIESIEGKRGMPRLRPPYVAQVGLFGRPTLEHNMETLYWVRDILERGADWFASQGR
                     HGRKGLRSFSVSGRVARPGVHLAPAGITVRELIDEYCGGMQAGHTFYGYLPGGASGGI
                     LPASLGDIPLDFDTLQPYGCFIGSAAVIVLSQHDRARDAALNLLEFFAEESCGQCTPC
                     RVGTQKAVGLMARPVWDKPLLDDLSQAMADASICGLGQAAPNPIACVYKYFPDELEPG
                     APGGAQEATT"
     gene            5258..8140
                     /gene="fdhF"
                     /locus_tag="VFO53_00090"
     CDS             5258..8140
                     /gene="fdhF"
                     /locus_tag="VFO53_00090"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HET8874700.1"
                     /translation="MNAPYDGPLGFLTAGNGPSPKARARARRVAPDAPLSPGPDIAFR
                     LNGADVLATPGETIIEVADRVGVSIPRLCYKPGYRPDGNCRACMVEIKGERVLAPSCC
                     RQPTAGMEVTSASTRAVHSQKMIVELLASDMPERSYKSDSELDYWQHALGIAKPRFAR
                     RAQPPSDLSHPAMAVNLDACIQCTRCVRACREEQVNDVIGYAFRSAHSKIVFDLDDPM
                     GLSTCVACGECVQACPTGALAPAQEAFLAPIDRKVASVCPYCGVGCQITYNIENDRIV
                     HVEGRDGPANRERLCVKGRFGFDYVHHPQRLTKPLIRKPGFPKSADFTVDPANPLSVF
                     REASWEEALALAGGTLARIRDTHGPGALAGFGSAKGSNEEAYLFQKLVRTGFRTNNVD
                     HCTRLCHASSVAALLEGIGSGAVSNPVMDVLQAEVVFLIGANPVSNHPVAATWMKNAV
                     KNGTKLILADPRRSELARHAAHYLQFKPDSDVAMLNAMMHTIIEEGLVDAKFIEDRTS
                     GYAELRDNVARFSPEAMAPVCGIDAPTLRTVARMFAKSRASMILWGMGISQHVHGTDN
                     ARCLIALSMITGQVGRPGTGLHPLRGQNNVQGASDVGLIPMMYPDYAPVGDAAVRQKF
                     EALWGARLDPNPGLTVVEIMHATHAGKIRGMYIMGENPAMSDPDVEHARAALAKLEML
                     VVQDLFLTETCYHADVILPASAFPEKTGTFTNTDRIVQLGREALPLPGDARKDLDIIV
                     AMARELGLDWHYTHPSEVFDEMREAMPSIAGITWDRLERDSGVTYPCENVGDPGEPVV
                     FIDDFPTQSGRARLVPAQIIPAAEKPDAEFPFVLITGRQLEHWHTGSMTRRSRALDAI
                     EPFAVASVNPGDLADLGVTPGGAITVASRRGSVSLYARADDGTPRGSVFIPFCYYEAA
                     ANLLTNPVLDPFGKIPEFKYCAVRVTAGGTPVATPGYREEETLAAD"
     gene            8181..8782
                     /locus_tag="VFO53_00095"
                     /pseudo
     CDS             8181..8782
                     /locus_tag="VFO53_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416684.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
     gene            complement(8835..11243)
                     /locus_tag="VFO53_00100"
     CDS             complement(8835..11243)
                     /locus_tag="VFO53_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET8874701.1"
                     /translation="MAFPTVPIRSLQSRILALFMLLLIVVQGGGYLLIKTAGIAAARK
                     SVSGEIAAGTLVFDRFLEQDTDRMVQGARLLTADYAFREAVATGDGETVASVLANHGK
                     RIDAALMMLIGLDRRVIADSIDSATGRPFGYPELLDRVQASQQARAMVLLGGELYQLV
                     IVPVLAPLPVAWLVVGFRVDDALAQDLHRLTRLQVSFLTRRHDEPWRIQASTLPQRER
                     AVLVAEAAAGSLRSAAGDTASYSDDAVTRVRNLSVQANGSVVAVLQEPLAVALEPFWQ
                     MQRRLTLISLFGVAVSIIASIAIARSIGRPVRDLAGVARRIASGDYTTVPSGRRNDEL
                     GDLALAFRAMQEGIMTRESRITDLAYRDALTGLPNRLLFADRLDQALAAAARAGLPLA
                     VLLMDLDHFKYVNDTLGHRIGDLLLKEVARRLQTVVRRATDTVARLGGDEFAILLAGA
                     SIGDARRLGEAILRALEAPMTLEEQIVDIAASIGIATCPDHGNESSKLLRCADVAMYE
                     AKRTSAGIAIWDDNYDQHSLERLSLMSDLRKAVDNDELVLVYQPKISVRPASELYVEA
                     LVRWQHPSRGLVPPSEFIPFAEQTGYIRVITQWVIARAIAQCTAWRRQGLPANIAINI
                     SARDLIDAQLPDRFEALLERERCAASWISLEITESAILDDPGHAIRNLERIAALGCRV
                     AIDDYGTGYSSLSYLRRLPLHELKIDRSFVTSLAHDANDAIIVRSTIELAHNMGLVVV
                     AEGVEDEATFDALRMLGCDTMQGYWISRPLGTAEAGQWLRESARLQSAAGAKGLRHVR
                     QVAS"
BASE COUNT         1877 a         3852 c         3754 g         1927 t
ORIGIN      
        1 gtagagtgcg cagacgatgc cgagggccag atgcagcccg gtgccgctgt cgccgatctg
       61 cgcaccggtc acgagcggcg ggccgtcgtc gaacccggtc gtcgacgccg agccgccggc
      121 gcattgcgcg acgttctcgt agaccttgca gtcagcgtat gggccgggcc cgaagccctt
      181 gatcgacgcg acgatcatct tcgggttgag ctcctggatg tgctcccagg tgagacccat
      241 tcgatcgagc gcccccggcg cgaaattctc gacgagcacg tcgcactccc tgacgagccg
      301 ctcgaggatc tccttgccct tcggattctt cgcgtcgatg gtgatcgagc gcttgttgtg
      361 gttcagcatc gtgaaataga ggctgtccac atcgggaatg tcgcggagct gtccgcgcgt
      421 gatgtcgcct tcacccgcgc gctcgacctt gatcacgtcg gcgccgaacc acgcgagaag
      481 ctgcgtgcat gtcggacccg actgcacgtg ggtgaagtcg aggatcttga ccccgtctag
      541 tgctttcgcc attgcaagcc ccaaggattg aaataaggta tgtgatatac caaggttcgt
      601 caaatgcgcc ggctgttcaa tgccgatccg cccgcgaccg ctcgatcccg ccgggggaac
      661 gcaaatgcgc ctcggtcagc tcacgttttc ttcgccgtcg ggttgagtcc cgtgatgcga
      721 ccgctttcgg ttcccgccct ctcgtcgatc accgcgttga cgagcgtcgg cttgcgcgag
      781 cgaatcgctt cttcgagggc cttgcgcaac tcgcccggcg tggtgacgtg cacgccgacg
      841 ccgccgaacg cctccatcaa cttttcgtag cgcgcgtcct tgacgaacac gagcggcgac
      901 ggatcgctct tcccggccca gttcgtttca tcgccgcggt agacgccgtt gttgttcagg
      961 atcacgacgc agacgggcag gtcatagcgg cagatcgtct cgacttccat gccggagaag
     1021 ccgaacgcgc tgtcgccttc gatcgcgatc acctgctcgc cgctcaccac ggccgcggcg
     1081 accgaaaagc ccatgccgat gcccatcacg ccccaggtgc cgacgtcgag gcgcttccgc
     1141 ggctcgtaca tgtcgacgat gctgcgcgtg aagtcgagcg cgttcgcgcc ttcgttgacg
     1201 aggatggcgc cggggttcgc cttgacgatg tcgcgcacga cgttgagcgc gccgtgaaag
     1261 ttcatcggcg tcgaattctt cgcgagcgtt tccgccatct tcgcgatgtt ccggctcttg
     1321 cgctcggcga tggcatcgag ccacgccgcg ggcggcttcg gccagcttgc gtcgattgcg
     1381 gccagcatcg ctgacacgca cgagccgatg tcgccgacga ccggcgcgtc gatgcgcacg
     1441 ttgctgtccg cctcggtcgg cgagatgtcg acctggatga atttctggcc gccccacgcc
     1501 ttggcgctct tgccacccca cgtcttgccc ttgccgtgcg acagcagcca gttgagccgc
     1561 gcgccgatca gcatgaccac gtcggcttcc ggcagcacgt acgagcgtgc cgccgcggcg
     1621 gattgcgcgt gcgtgtcggg cagcaggccc ttcgccatcg acatcggcaa atagggtatg
     1681 ccggtctttt cgatcagcgc gcggatgtcg gcgtcggcct gcgcgtaggc ggcgcccttg
     1741 ccgaggatga tcagcggctt cgccgcaccc ttcaagaggt cgagtgcacg cgtaaccgcg
     1801 tcctgcgacg ggatctggcg cggcgccgga tcgacgacct tgatcaacga ctgcctcccc
     1861 gcttcggcat ccattgcctg cgggaacagc ttcgccggca ggtcgagata gacgcccccc
     1921 gggcggcccg aaacggcggc acggatcgcc cgtgcgacgc cgacaccgat gtcgtcggcg
     1981 tgcagcacgc gaaaggccgc cttcgcgagc ggcttcgcga tcgcgagctg gtccatctcc
     2041 tcgtagtcgc cctgctgcag gtcgacgacc tcgcgctcgc tcgagccgct gatcaggatc
     2101 atcgggaagc agttcgtcgt cgcgttggcg agcgccgtca ggccgttgag gaagcccggc
     2161 gccgagacgg taaggcagat gcccggcttt tgcgtcatga agccggcgat ggacgcggcg
     2221 tagccggcgt tctgctcatg gcgaaacgag atcacgcgca ggccggccgc ctgggccttc
     2281 cgcgtgaggt cggtgatcgg gatgccgggc aaaccgaaga tcgtatcgat gccattgagc
     2341 ttgagagcat cgatcagcag gtgaaagccg tcgatctccg gccgggattg cacattggcc
     2401 tggccaccgc tcgtggcgag gccagactcg gtcgcggcgg atgccgctcc cttgcgcgtt
     2461 gcggtgacgc tttccatggg aatctcctga atcgaggcag cttggcgtgt tggacccgac
     2521 cgggcgccgc gccgtgcgcc accgtcgcga gaaatacggg ttcgccggaa gcccaagctt
     2581 ccgcaaacat tcgcataacg tatgtgatat accagacgcc gtcaatggcc ggtttcggaa
     2641 cctctccacc ggtagagtgc cggtgcgacc ggtgcccggc gccatttcgg ccccacgccc
     2701 ggctccgcgc gtcaccgccg ctaccccgct cgtggttttt ggtatataac atacttgaac
     2761 caggagcggc agcactccgc accgcccgcc gagagaggac ccaaaacagt gtcaacgggc
     2821 ccggctggag ccggcacgag cgcgattcca ctcgcgttgc gaccgctttc cgccaacgtc
     2881 agcttccgcg atcaggccta tgccgcgctg aagcaggcga tcacggaagc ggatatctat
     2941 tcgcatccgc acgagctgcg cctcgacgag cgccagctgt cgcgctcgct cggcgtgtcg
     3001 cgcaccccga tccgcgaggc gatgacggtg ctcgagcagg aagggttcct gcgcaccgtc
     3061 ccgcggcggg gcatcttcat cgtgcgcaag acgaagcgcc agatcatcga aatgatcgaa
     3121 atgtgggcgg cgctcgagag catggcggcg cgtcttgcga cgctcaacgc ctcggacgcc
     3181 gaaatcgcga cgttgcgcac gatgttcgac gagtttcgca attcgacgcc ggccgagcac
     3241 atcgacgagt actcgcacgc gaacatcgcg tttcaccagg cgatcatccg gctctccggc
     3301 tcgcacctga tgggcaagac catcgagaac ctgttcattc acgtgcgcgc catgcgccgg
     3361 ctcacgatct cgcaaagcga ccgtgcggcg cgctcgatcg tcgaccacat gcgcatcatc
     3421 gaggcgctcg agcgccgcga taccgagcta gccgagcggc tcgtgcgcga tcattcgctc
     3481 gacctcgcgg cattcgtcga gaagcactgc gactttttgg aataggcgcc atgaccgatc
     3541 cggttgtcgt cgatacgtcg aagctgcggg gcggccgcgg cgtgaaagcc cggcccaaag
     3601 gccgccatgt cgatgcgcaa gcgctggccg acgtgcagcg cctgctcggc gacatgccgc
     3661 gcgcgcggtc gctgctgatc gaatgcctgc atcgcatcca ggacgcgcgc ggctacctgc
     3721 cggcgtcgta tctggttgcg ctcgcgcagg agatgaagct cgcgatgacg gaagtgtacg
     3781 aagtggcgac gttctatcac cacttcgacg tggtcgatgc cgacgatgcg ccgccgcccc
     3841 cgatcaccgt ccgcgtctgc gaaacgctgt cgtgcaagat ggccggcgcc gacgcgctcc
     3901 gcgcaacgct ggttgcgcat ggggatgcca acgtgcgcgt gctcggcgcg ccatgcatcg
     3961 gccgctgcga actggcgccc gccgccgtcg tcggccgcaa cccgatcggt cacgccacgc
     4021 ccgagcgcat tctcgacgcg atccgcaccc gggcgctcga accggcgccg gctccgtacg
     4081 tcgactacgc cgcgtatcgg cgcgacggcg gctatcgcac gctcgtcgat tgcgtcggcg
     4141 gccaacgcag cgtcgaggcg gtgatcgccg aaatggagac ttcgtcgctg cgaggcctcg
     4201 gcggcgccgg attcccggcc ggacgcaagt ggaagatcgt ccgcgccgag ccggcaccgc
     4261 ggctcatggc ggtcaacatc gacgaaggcg agcccggtac cttcaaggat cgctactacc
     4321 tcgagcgcga cccgcaccgc ttcctcgacg ggatgctgat cgccgcgtgg gccgtgggca
     4381 tcgacgcgat ctacgtttac cttcgcgacg aatacgccgg atgccgggca atcctccagc
     4441 gcgagatcga cgcgctcgtc gccgacccgc cgtgtccgtt gccgcgcatc gaattgcgcc
     4501 gcggcgccgg cgcgtacatc tgcggtgaag agtcggccat gatcgaatcg atcgagggca
     4561 agcgcggcat gccgcgcctt cgtccgccgt atgtcgcgca ggtcgggctc ttcggccggc
     4621 cgacgctcga gcacaacatg gaaacgctct actgggtgcg cgacatcctc gagcgcggcg
     4681 ccgactggtt cgcgagccag ggacgacacg gccgcaaggg attgcgctcg ttctcggtgt
     4741 cgggacgcgt tgccaggccc ggcgtgcatc tggcacccgc cggcatcacc gtgcgcgagc
     4801 tgatcgacga gtactgcggc ggcatgcagg ccggccacac gttctacggc tacctgccgg
     4861 gcggcgcgtc gggcggcatc ctgccggcat cgcttggcga cattccgctc gacttcgaca
     4921 cgctgcaacc ctacggctgc ttcatcggct cggccgccgt gatcgtgctg tcgcaacacg
     4981 atcgtgcgcg cgacgccgcg ctgaacctgc tcgaattctt cgccgaagag tcgtgcggcc
     5041 agtgcacgcc gtgccgcgtc ggcacgcaga aggcggtcgg cctcatggca cggccggtct
     5101 gggacaagcc gctgctcgac gacctttcgc aggcgatggc cgatgcgtcg atctgcggtc
     5161 tcgggcaggc ggcaccgaac ccgatcgcgt gtgtctacaa gtatttcccc gacgagctcg
     5221 aaccgggcgc gccgggcggc gcacaggagg cgacgacatg aatgcacctt acgacggacc
     5281 gctcggcttc ctcaccgcgg gcaacggccc gagccccaag gcgcgtgcgc gcgcacgccg
     5341 cgttgccccc gacgcgccac tttcgcccgg acccgacatc gcgtttcgcc tcaacggcgc
     5401 ggacgtgctc gcgacgccgg gggaaacgat catcgaggtt gccgatcgcg ttggcgtgtc
     5461 gatcccgcgc ctttgctaca agcccggcta tcgtcccgac gggaactgcc gcgcgtgcat
     5521 ggtcgagatc aagggcgagc gcgtgctcgc gccgtcgtgc tgccgccagc cgaccgccgg
     5581 gatggaggtg acgagcgcca gcacgcgtgc cgtgcattcg cagaagatga tcgtcgagct
     5641 cctcgcgtcg gacatgcccg agcgctcgta caaaagcgat tcggagctcg attactggca
     5701 acacgcgctc ggaatcgcga agccccgctt tgcgcgccgc gcgcaaccac cgtcggatct
     5761 gtcgcatccg gcaatggccg tgaatctcga tgcatgcatc cagtgcacgc gctgcgtgcg
     5821 cgcctgccgc gaggagcagg tcaacgacgt gatcggctac gcgtttcgct cggcgcattc
     5881 gaagatcgtg ttcgatctcg atgatccgat gggcttgtcc acctgcgtcg cctgcggcga
     5941 gtgcgtgcag gcgtgcccca ccggcgccct ggcgcctgca caggaggcgt tcctcgcgcc
     6001 gatcgaccgc aaggtcgctt cggtgtgccc gtactgcggc gtcggctgcc agatcaccta
     6061 caacatcgag aacgaccgta tcgtccacgt ggaaggccgc gacgggccgg ccaaccgcga
     6121 acgcctttgc gtcaagggcc gcttcggctt cgactacgtc caccacccgc agcggctgac
     6181 gaagccgctg attcgcaagc cgggcttccc gaagtcggcc gacttcaccg tcgaccccgc
     6241 gaatccgctg tcggtgttcc gcgaagcgtc gtgggaggaa gcactcgcgc tggccggcgg
     6301 cacgctcgcg cgcatccgcg acacgcacgg ccccggcgcg ctcgcgggct tcggctccgc
     6361 gaagggcagc aacgaggaag cgtatctgtt ccagaagctc gtgcgcaccg gatttcgcac
     6421 gaacaacgtc gatcattgca cgcggctttg ccacgcgtcg agcgtcgcgg cgctgctcga
     6481 aggcatcggc tcgggtgcgg tttccaatcc ggtgatggac gtgctgcagg ccgaggtcgt
     6541 gttcctgatc ggcgcgaacc cggtgtccaa ccacccggtg gccgcgacat ggatgaagaa
     6601 cgcggtcaag aacggcacga agctgatcct cgccgacccg cggcgctcgg agctcgcgcg
     6661 ccacgccgcg cattacctgc agttcaagcc cgacagcgac gtcgcgatgc tgaacgcgat
     6721 gatgcacacg atcatcgagg aagggctcgt cgatgcgaag ttcatcgagg accgcacgag
     6781 cggctacgcc gagcttcgcg acaacgtcgc gcgcttcagc cccgaggcga tggcgccggt
     6841 ttgcggcatc gacgcgccta cgctgcgcac cgtcgcgcgg atgttcgcga agtcgcgcgc
     6901 gtcgatgatc ctgtggggga tgggcatctc gcagcacgtg cacggcaccg acaacgcgcg
     6961 ctgcctgatc gcgctgtcga tgatcaccgg ccaggtcggg cgtccgggca ccgggctgca
     7021 cccgctgcgc ggacagaaca acgtgcaggg cgcctccgac gtcggattga tcccgatgat
     7081 gtaccccgat tacgcaccgg tcggcgacgc cgccgtccgc cagaaattcg aagcgctgtg
     7141 gggcgcccgg ctcgatccga atccgggact caccgtcgtc gagatcatgc atgcgacgca
     7201 cgccgggaag atccgcggca tgtacatcat gggcgagaac ccggcgatgt cggatcccga
     7261 tgtcgagcac gcacgcgcgg cgctggcgaa gctcgagatg ctcgtcgtgc aggatctctt
     7321 cctcaccgaa acctgctatc acgccgacgt catcctgccc gcctcggcat ttcccgagaa
     7381 gaccgggacg ttcaccaaca ccgatcgaat cgtgcaactc ggccgcgaag cgctcccgtt
     7441 gccgggcgat gcgcgcaagg acctcgacat catcgtcgcg atggcgcgcg agctcgggct
     7501 cgactggcat tacacgcatc cgagcgaggt gttcgatgaa atgcgcgagg cgatgccgtc
     7561 gatcgccggc atcacgtggg accgcctcga gcgcgacagc ggcgtcacct atccgtgcga
     7621 gaacgtcggc gatcctggcg agccggtcgt gttcatcgac gattttccga cgcaaagcgg
     7681 gcgggcgcgc ctcgtgccgg cgcaaatcat tccggcggcg gaaaagcccg atgccgaatt
     7741 cccgttcgtg ctcatcaccg ggcgccagct cgagcattgg cacacgggca gcatgacgcg
     7801 gcgcagccgc gcgctcgatg cgatcgagcc cttcgccgtg gcgtcggtca atccgggcga
     7861 tctcgccgat ctgggcgtca ccccgggcgg cgccatcacg gtggcgtcgc gaagaggcag
     7921 cgtgtcgctc tatgcgcgag ccgacgacgg tacaccgcgg ggcagcgtgt tcattccgtt
     7981 ctgctactac gaggcggcgg cgaacctgct caccaacccg gtgctcgatc cgttcggcaa
     8041 gatccccgag ttcaagtatt gtgcggtgcg cgtgaccgcg ggcggcacgc cggtggctac
     8101 acccgggtat cgcgaagagg aaacgctggc cgccgattga accggcgcct cgagggcgcg
     8161 cgcagacgac aggttcggcg atgaacaagg cccggctgga ggcgttcagc gatggcgtga
     8221 tcgcgatcat catcacgatc atggtcctcg agctgcaccc gccgcacgcg gaggacctgt
     8281 cggcgctcgt gccgctcgtt ccggtattcc tctcctacgt gctgagcttc atctatctcg
     8341 gcatctactg gaacaaccat caccacatgc tgcatgcggc gcagacggtg attggtccga
     8401 tcctctgggc gaacctgcac ctgctgttct ggctgtcgct cgtccattca cgaccggctg
     8461 gatgggcgag aaccactttg cgagcgcgcc gtctgcgctg tacggtttcg tgctgctgat
     8521 ggcggccatc gcgtacgtga tcctccagca gcgcatcatc gccgcggagg ggagcgactc
     8581 gttgctgcgc aaggcgatcg gcagcgactg gaaaggcaag ctgtcgcccg tgatgtatct
     8641 cgccgcgatc gtgctttcct tcgtgtcgca ctggattgcg caggcgctgt acgtcgcggt
     8701 tgcgctgatg tggctggtgc ccgaccgcag gatcgagcgg gcgctggcgg gcggcgaccg
     8761 ttatcgcgag cggcgggcgt gagcgtggcg cgcgcgccgg atcgcgggtc gcacagcgaa
     8821 ggtcgaccgc gcgatcacga tgccacctgg cggacgtgtc gcaggccctt ggcgccagcg
     8881 gcggactgca agcgcgccga ttcacgcagc cattgcccgg cttccgccgt tccgagcggc
     8941 cggctgatcc agtaaccttg catcgtgtcg caaccgagca tgcgcagcgc gtcgaacgtc
     9001 gcctcgtcct cgacgccctc ggcgacgacg accagtccca tgttgtgggc gagctcgatc
     9061 gtcgagcgca cgatgatcgc gtcgtttgca tcgtgcgcca gtgacgtcac gaacgaccgg
     9121 tcgatcttga gctcgtgcag cggcagccgc cgcaagtacg agagcgacga atagccggtg
     9181 ccgtagtcgt cgatcgcgac cctgcacccc agcgcggcaa tgcgctcgag atttcggatt
     9241 gcgtgacccg gatcgtcgag gatcgcgctt tcggtgatct ccagcgatat ccagctcgcc
     9301 gcgcagcgct cgcgctcaag cagtgcctcg aaccggtcgg gcaactgcgc atcgatcagg
     9361 tcgcgcgccg aaatgttgat cgcaatgttt gccggcagtc cctggcgccg ccatgccgtg
     9421 cactgcgcta tggcgcgcgc gatgacccat tgcgtgatga cgcgtatata gcccgtctgc
     9481 tcggcgaacg gaatgaattc ggacggcggc acgagaccgc gcgacggatg ttgccagcgc
     9541 accagcgcct cgacgtagag ctcgctggcg ggacgcaccg agattttggg ctggtacacg
     9601 agcacgagct cgtcgttgtc gaccgccttg cgcagatcgc tcatcagcga caaccgttcg
     9661 aggctgtgct gatcatagtt gtcgtcccag atcgcgatgc ccgcgctggt ccgcttcgcc
     9721 tcgtacatcg cgacgtcggc gcagcgcaac agcttcgacg attcgttgcc atgatcggga
     9781 cacgttgcga taccgatgct ggcggcgata tcgacgatct gctcttcgag cgtcatcggc
     9841 gcctcgagcg cccgtaggat cgcctcgccg aggcgccgcg cgtcgccgat ggaggcgccg
     9901 gctagcagga tcgcgaactc gtctccgccg aggcgggcga cggtatcggt ggcccgccgg
     9961 acgacggtct gcaggcgccg ggcaacctcc ttcagcaaca ggtcgccgat gcgatggccg
    10021 agcgtatcgt tgacgtactt gaagtggtcg aggtccatca gcagcaccgc gagcggcaaa
    10081 cccgcgcgtg cggcggcagc aagtgcctga tcgaggcgat ccgcgaacaa caggcggttc
    10141 ggcagtccgg tcagcgcgtc gcggtacgcg aggtccgtga tcctcgattc gcgcgtcatg
    10201 atgccctcct gcatcgcgcg aaacgccagg gcgaggtctc ccagctcatc gttgcgtcgg
    10261 ccggacggca ccgtcgtgta gtcccctgac gcgatgcgcc gagcgactcc ggcgaggtca
    10321 cgcacggggc gcccgatgct gcgggcaatg gcaatgcttg cgatgatcga gacggcgacg
    10381 ccgaacagcg agatcagcgt aaggcgtcgt tgcatctgcc agaacggttc gagcgctacg
    10441 gccaacggct cctgcagcac ggcgacaacg cttccgttgg cctgcacgga gagattgcgc
    10501 acgcgcgtga ccgcgtcatc gctgtagctc gccgtgtcac ccgccgcgct gcgcagactg
    10561 ccggctgctg cctctgcgac gagcacggcc cgctcgcgct gcggcaacgt gcttgcctgg
    10621 atgcgccacg gctcatcgtg gcgccgggtc agaaaggaaa cctggaggcg cgtcaaccgg
    10681 tgcagatcct gcgccaatgc gtcgtcgacc ctgaagccga cgacgagcca cgcaacgggc
    10741 aatggcgcaa gcacggggac gatcacgagc tgatacagct caccgcccag caacaccatc
    10801 gctcgcgcct gctgggatgc ctggaccctg tcgagcagct ccggatatcc gaacggccgg
    10861 ccggtcgcac tgtcgatcga gtcggcgatc acgcgccggt cgagaccgat cagcatcatc
    10921 aacgcggcgt cgatccgctt gccgtgattc gcgagcaccg aggcgaccgt ctcgccgtcg
    10981 ccggtcgcca ccgcttcgcg aaacgcatag tcggcggtga gcaggcgtgc gccctggacc
    11041 atgcggtcgg tgtcctgctc gaggaaacgg tcgaaaacga gcgttcctgc cgcgatttcg
    11101 ccgctgaccg atttacgcgc cgccgcgata ccggctgtct tgatcagcag atagccgccg
    11161 ccttgcacga cgatcaacag cagcataaac agcgcgagga tgcgcgactg gaggctgcgg
    11221 atcggtacgg tcggaaaggc catcggaggt gccgcgtttc acgctcagcg cgcgctcgag
    11281 tgtccgcgaa gggcaccggc tgtcgcgacg aggcatccgg cacgcgcgcg cgtgcattga
    11341 acagaatgtg ctgtacatcg acgaggcggt ggcggcatcg cagtggtggc gcttggtcag
    11401 cgacagggcg
//
LOCUS       DASSVX010000004        35163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_749967, whole genome shotgun sequence.
ACCESSION   DASSVX010000004 DASSVX010000000
VERSION     DASSVX010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 35163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35163
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749967"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1007)
                     /gene="mlaE"
                     /locus_tag="VFO53_00105"
     CDS             complement(186..1007)
                     /gene="mlaE"
                     /locus_tag="VFO53_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HET8874702.1"
                     /translation="MSAAGGRGVSDSLAWALRRVGSATIDSVARLGFAARFSIAILRH
                     LPGAFRRLQLTLREIYFSGVLSLVIILVSGLFVGMVLALQGYDVLSRYGANESLGILV
                     ALSLVRELGPVVAGLLFASRAGSAVTAEIGLMKTTEQLSAMEMMAVDPLARVVAPRFV
                     GGVVSMPLLAALFSTLGVFGAYIVGVRLIGIDSGSFWGNMQAAVDFRYDIVNGFIKSV
                     VFGIAVSAIAVFEGYNARPTAEGVSTATTRTVVASSLSILALDFVLTVFMFRGVH"
     gene            complement(1004..1837)
                     /locus_tag="VFO53_00110"
     CDS             complement(1004..1837)
                     /locus_tag="VFO53_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8874703.1"
                     /translation="MSVAEPAAVSPTCPTEIAVRLTGVSFGYDPKRPVLRGIDLAIPR
                     GRLVAIMGGSGCGKTTILRLIGGQLRPQAGTISVAGEDVASLDRDALFALRRRIGMLF
                     QFGALFTDMSVFENIAFPLREHTELQEELVRDLVLMKLNAVGLRGAAQLRPSELSGGM
                     ARRVALARAIALDPMLVMYDEPFAGLDPISLGIVGQLIRRLNDALGITSIVVTHDIYE
                     SLKIVDYIYFVSEGRIIAQGTPDEVRASSDAFVRQFVHGEPDGPVPFHYPAKAVKEEF
                     A"
     gene            1951..2979
                     /locus_tag="VFO53_00115"
     CDS             1951..2979
                     /locus_tag="VFO53_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HET8874704.1"
                     /translation="MIADADARSDARDGAPRTVDAERALNLARNVLDTEARAISALSA
                     RLSAPFVAAVDVMLGCRGRVVVLGIGKSGHIARKLAATLASTGTPAFFVHPAEAGHGD
                     LGMITADDVVVMLSNSGETDELLLLTPHLKRQGAKLVALTGNEKSSIAQSADVHLDAA
                     VDAEACPLGLAPTASTTAALALGDALALALLDARGFSVHDFARSHPGGALGRRLLTRV
                     ADVMRTGAALPRVAHTATLAEAIVEMTAKGLGMTAVVDARNRIMGVFTDGDLRRSLTR
                     VVDATTARVADLMTKSPRTIGAERLAADCIVLMETPPKITVLLVAGDAGELVGAVQMH
                     DLFRARVL"
     gene            3015..3554
                     /locus_tag="VFO53_00120"
     CDS             3015..3554
                     /locus_tag="VFO53_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012847373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET8874705.1"
                     /translation="MRARRVRLLTCDVDGVLTDGRIYVDDQGRELKAFSALDGVAMHR
                     LAAAGIVVAWITGSAAAAVTHRAQQLRIPHLVLDAQDKLVPWERLRAELGLAADACAH
                     IGDDLADIPVLAACGLGVTVPHAPSVVRANAHYVTVRDGGHGAVRELADMILAAQGYD
                     DAAGVAPPRASAALASPVR"
     gene            3569..4195
                     /gene="lptC"
                     /locus_tag="VFO53_00125"
     CDS             3569..4195
                     /gene="lptC"
                     /locus_tag="VFO53_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HET8874706.1"
                     /translation="MDRSRALFDRLVAWSPVLLLGGLAALTYWLDAQVQTATTHRDGS
                     TRHDPDLFLRDFKAVTFDVHGLPRESLSAASAEHFPDNDSAELRAPRLSLTEPGRPTM
                     TITAERGVIAGDREHGDFMGDVRVERQADPAPSKPGDDASGPVTLTTSLLHVFTKEQR
                     VTTDQPVTIEEPRGIIRGQGLEFDNKAKRVRILSHVSGTLSPQRQPPR"
     gene            4192..4926
                     /gene="lptA"
                     /locus_tag="VFO53_00130"
     CDS             4192..4926
                     /gene="lptA"
                     /locus_tag="VFO53_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HET8874707.1"
                     /translation="MTPYGRSSAPVRATLACVVMLLAAAPVDAEKADREQNINFSAEQ
                     PVEVDFDKRVGTLRGNVVITQGTLTIRADKIDFKQNSDDSLSATAWGNPIRFREKKDD
                     SDEYYEGFAQRAVYNGQTHTLELFDNALLKEGNNEFRGNYISYNSDTNFMKAEGRPNA
                     PGAEGPGARVQGVFEPRSEGPPGTRGAPKANEKTPDGTKAAPKAEATGADGRKSGTSK
                     SSAGARTEAKAAPPLALTPDPTLPAK"
     gene            4965..5723
                     /gene="lptB"
                     /locus_tag="VFO53_00135"
     CDS             4965..5723
                     /gene="lptB"
                     /locus_tag="VFO53_00135"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HET8874708.1"
                     /translation="MERRPSHAPGDAPSKLAAAHLRKRYKARTVVHDVSLDVASGEVV
                     GLLGPNGAGKTTCFYMIVGLVAADGGDISLDGENLSRLPIHRRARRGLSYLPQEASIF
                     RKMTVAQNVRAILELQDLDPDTLSNRLDALLDDLTISHLADAPAVSLSGGERRRVEIA
                     RALATRPRFILLDEPFAGVDPIAVLDIQKIIGFLKERGIGVLITDHNVRETLGICDRA
                     YIINEGRVLASGAPGEIVYNEDVRKVYLGEHFRL"
     gene            5851..7326
                     /locus_tag="VFO53_00140"
     CDS             5851..7326
                     /locus_tag="VFO53_00140"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET8874709.1"
                     /translation="MKQTLQLKLSQHLTLTPQLQQSIRLLQLSTIELNAEVERMLQEN
                     PLLERAEVEEDRIAAEVPLSGPGRVGDGVDHATHDGTAGESAAEIRSTRDDAPEVVDF
                     ADYGGSSGDSDWGGSNSNDDDDFLPQQIATSTLRDHLNGQLALLNLPLRDRHIVTALI
                     DALDDDGYLATPLDEIAELFPAELEIDADDLNIALKYVQSFEPSGVGARDCAECLALQ
                     LRALPAATPFLADALKVVDGNLALLANRDFTKLKRLLHTDDTGVRGVRDLIRSLNPRP
                     GAAFAKAEANYVIPDVVVRKIRNQWVASLNEAAMPKLRLNRIYADILTRNRESSNQQL
                     AAQLQEAKWLIRNVQQRFDTILRVAKAIVERQRRFFEHGEVAMRPMVLREIADMLSLH
                     ESTISRVTTQKYMLTPRGTFELKYFFGSHVATDTGGAASATAIRALIKQLIAAEDAKT
                     PLTDSKIADMLGEQGILVARRTIAKYREALQIPPVNMRKAL"
     gene            7340..7687
                     /gene="raiA"
                     /locus_tag="VFO53_00145"
     CDS             7340..7687
                     /gene="raiA"
                     /locus_tag="VFO53_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET8874710.1"
                     /translation="MNLHLTGHHVDITPAIRDYVIAKLNRINRHFDHVIDVSVVLTVD
                     RLSHKVEATLHVRGKDLHAECIDADMYAAIDGLADKLDRQVLKYKEQIKDHRTDTEAI
                     NRSAAETGTEGTS"
     gene            7787..8248
                     /locus_tag="VFO53_00150"
     CDS             7787..8248
                     /locus_tag="VFO53_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET8874711.1"
                     /translation="MNQIAALLPASNILVDLDVASKAALFGTMGEVFERSAGLPRAGV
                     AASLAAREKLGSTGLGQGIAIPHGRIARLSAAIGAFARLRAPIAFDAPDGKPVDQAFV
                     LLVPEQATDQHLQLLSELAQMFSERAFRDRLMAAKDAAALAGIFRGWQPQD"
     gene            8263..9201
                     /gene="hprK"
                     /locus_tag="VFO53_00155"
     CDS             8263..9201
                     /gene="hprK"
                     /locus_tag="VFO53_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HET8874712.1"
                     /translation="MRQVSVERFLEDNRERLGLVWLAGRDGGDRMLVGESALRPTIGQ
                     IGHMNLIHPFRVQILGAAELAYLRALPEDELNRSINRMFTTELAAIVVGNGEQVPRHL
                     LDMCNQHRIALVTSQQPSPHIVDVFRIYLSRVLAESTTLHGVFLDVLELGVLITGASA
                     IGKSELALELISRGNGLIADDIVELHRISPDTIEGRCPAVLRDFLEVRGIGILNIRTI
                     FGETAVRPHKLLRLIVHLEDHGNEAFSDLDRLQVNATFQEVLSVPIRKVTIPVAAGRN
                     LAVLVEAAVRNFVLNQRGIDSTKEFIERQQKLMDEG"
     gene            9212..10090
                     /gene="rapZ"
                     /locus_tag="VFO53_00160"
     CDS             9212..10090
                     /gene="rapZ"
                     /locus_tag="VFO53_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET8874713.1"
                     /translation="MLIGGVSGSGKSVALAAFEDAGYYAVNNLPVPLLFDTASYLEGS
                     GQERVAIALDVKSGPGLPGLAEAMAALEQRGWKVRLLYLDATVETLVKRFSETRRRHP
                     FSSGDRTLTEAIEYERALLAHARQLGTTFDTSELPAAHLRGWIKDFVSVDASQITLQL
                     ESFGFKHGVPLDADLVFDARCLPNPHYEPALSPLTGKHPGVVAFLEAIPEVERMYGDI
                     YHFIASWLPDYARDNRNYLTVAIGCTGGQHRSVYMVERLLQAFSPRYQVLVRHRELAP
                     GDAPATDGASSPATPG"
     gene            10087..10713
                     /locus_tag="VFO53_00165"
     CDS             10087..10713
                     /locus_tag="VFO53_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET8874714.1"
                     /translation="MSEPAARASSSLPLFPLRTVLFPGGLLPLKVFEQRYIEMTKACL
                     RDERPFGVCLITHGDEVARGDGAFPEIAKVGTLARITSVDMPQMGILHLSTAGGARFE
                     VRSHAPDALGILVASVTPIDDEPRHPLAETYAPLARLLEVIATRLGASNFPAATHYDD
                     ASWVGYRLAELLPLPLSIKQSMLEVSDANVRLAALKQFLERQGLLQAV"
     gene            10747..11475
                     /locus_tag="VFO53_00170"
     CDS             10747..11475
                     /locus_tag="VFO53_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET8874715.1"
                     /translation="MAGHSKWANIKHKKAAADAKRGKVFTRLIKEITVAARLGGGDAS
                     ANPRLRLAMDKAMGQNMPKDTIERAIKRGAGGLEGVNYEEIRYEGYGIGGAAVIVDCM
                     TDNRTRTVADVRHAFSKYGGNLGTDGSVAFLFRHVGQILFAPGSDEDKVMEAAIDAGA
                     DDVVTHEDGSIEVLTAPNDFIAVKDALAKSGLAPEFAELTMKPLTEVDMRGDDAQRMQ
                     KLLDALESLDDVQDVYTSAAIEEA"
     gene            11510..12043
                     /gene="ruvC"
                     /locus_tag="VFO53_00175"
     CDS             11510..12043
                     /gene="ruvC"
                     /locus_tag="VFO53_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET8874716.1"
                     /translation="MGVRRILGIDPGLRVTGFGILEARGATLVYVSSGCIRATGSSLP
                     IRLGVIVRDLAHVIAEERPTEVAVEKVFVNINPNSTLLLGQARGAAIAAAVIAGLPVS
                     EYTAGQVKQAVVGAGRALKPQVQEMVRRLLSLPGIPASDAADALAAAICHAHASTGVG
                     ALARHGYRVRGGRLLVT"
     gene            12072..12653
                     /gene="ruvA"
                     /locus_tag="VFO53_00180"
     CDS             12072..12653
                     /gene="ruvA"
                     /locus_tag="VFO53_00180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET8874717.1"
                     /translation="MIGRLSGTLLEKNPPQLLLDVQGVAYEVDVPMSTFYNLPANGER
                     VTLFTHLVVREDAHLLYGFGSDNERRAFRQLLKVNGIGAKIALSLLSGLSVTELAQAI
                     AMQETGRLTRVPGIGRKTAERLLLELKDKLGTELTHAIGVNRAPPATSDIMHALIALG
                     YSEREAAAAIRQLPDGVAVADGIKQALKLLAKP"
     gene            12721..13767
                     /gene="ruvB"
                     /locus_tag="VFO53_00185"
     CDS             12721..13767
                     /gene="ruvB"
                     /locus_tag="VFO53_00185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET8874718.1"
                     /translation="MAIENDELTRIVATNSTGTQEEALERALRPRRLAEYIGQEKIRD
                     QLGIFIEAARRRSEALDHVLLFGPPGLGKTTLSHILAHELGVNLRQTSGPVLERPGDL
                     AAILTNLEPRDVLFIDEIHRLSPIVEEILYPALEDFQIDIMIGEGPAARSVKIDLPPF
                     TLVGATTRAGMLTNPLRDRFGIVARLEFYSPQELTQIVARSARLLSMLVEPDGALEIA
                     RRARGTPRIANRLLRRVRDYAEVRAGGDVTRHVADAALSMLDVDASGLDTMDRKLLST
                     VLDKFGGGPVGVDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTSRGRVATPLAYRHFG
                     LAAPAAGGDLFPGQ"
     gene            13844..14512
                     /gene="tolQ"
                     /locus_tag="VFO53_00190"
     CDS             13844..14512
                     /gene="tolQ"
                     /locus_tag="VFO53_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HET8874719.1"
                     /translation="MNVHTDLSFLTLIIHASVIVQGIIALLALLSMWSWWQIFLKMFQ
                     LRRIARETDAFEDEFWQGGNLETLYRQAREAQVGGGLEQIFTAGFREFSKHRRQGSAA
                     DVTLDAARRAMRATYQREIDAIEAHLAGLATVGSVSPYIGLFGTVWGIMNAFRGLANV
                     SQATLANVAPGIAEALIATAIGLFAAIPAVVAYNRFTHDVDRLAVRFETFIEEFSNIL
                     QRQG"
     gene            14570..14974
                     /locus_tag="VFO53_00195"
     CDS             14570..14974
                     /locus_tag="VFO53_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET8874720.1"
                     /translation="MRRARKPINQINVVPYIDVMLVLLVIFMVTAPLVAPGEIQLPSV
                     GSKLTNPDNPLNVSLKGTTITLTDKLAKTERRVSKDQLVADVLAAQAGQERPVVIAAD
                     KDARYQDVLDILDILQRSGVKRVGLLARPPAA"
     gene            14986..15945
                     /gene="tolA"
                     /locus_tag="VFO53_00200"
     CDS             14986..15945
                     /gene="tolA"
                     /locus_tag="VFO53_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HET8874721.1"
                     /translation="MSPHERPAPAQRARGKWTALILALFVNLLFVGVLVFSVSWQNRP
                     PQAVTAELYAPPPPTVTAPRRVEPKPQPKLEPIPPKPEPLPPKLEPMPKPPEPAVAKP
                     DTQAADIALKARREAERIRQEQVERENHRQEEARRQEEAEKRDEARRQAAARQAAEDK
                     RQAELRERQQRQLETMRAQAAQEAQMRTQAERESQMRAQAERESRLRAEAEREARGRA
                     EQAAAGARNKAQLDWIDKIRSRIRGNINLPPDIPGNPEAVFDVVQLPTGEIIDVKLRK
                     SSGVRAYDEAVQRAILKSSPLPKPVPADLFQRSLELRFRPLDQ"
     gene            complement(15953..17665)
                     /locus_tag="VFO53_00205"
     CDS             complement(15953..17665)
                     /locus_tag="VFO53_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874722.1"
                     /translation="MGAMFAFLSSAPRDTADPIVSVKSAATWLRQLPSLDAVARQQLV
                     VRAFDGMRQSRRPVDLARAQALQYIDAALGADRRQLFRQYVESLESAPKVSERIWQAS
                     LDLAQAFIAAYQRTLEEALAPAAYGRWKPHVPILFARLVHYYGTDAKLRVCRHEHWIP
                     AKWLELHRAYMRATELGVDRVPTSLGASGGNGTQWTVEQEYVYALLLHQLNSGNLSPA
                     NLDWAASQVRGFSRKLELVALPKTMEGFFVDVAGRTGLTRRTGQDSGSMLRYLDTTLL
                     ASQLDMSLAALRHSEETDQGPVGPINQQRAMILEKARAAIAPNLNTDMRRDPRTPCVV
                     AARVRVGLARIQHELNKPQKDAPVGDTTSGEEIEVYAVTGPARTKPQLVDEADTLSSS
                     LATFSDPVWQVKDRSLAGLRICASGGVGQTLMLGALVAVRQSDASHWLLGVVRRLNKV
                     SVEDVEAGVSLIAERVVAVTLYAKREGKQELGIVVNGLDTSMMGPRFEGLYLPPPSRP
                     DKPLVVKTLIVPTHEYAEGRKLILITGRSVYTVALRQLVEQRGDWSWTAIQIVEKQPR
                     TGAE"
     gene            17952..18377
                     /locus_tag="VFO53_00210"
     CDS             17952..18377
                     /locus_tag="VFO53_00210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET8874723.1"
                     /translation="MAIGCTATAATAPTSASEGQSKLNATPENLQKIDVKQGTGTEAV
                     AGHTVVVHYTGWLYDSSKPDSHGAKFDSSRDRNAPFSFALGAGRVIKGWDQGVAGMKV
                     GGQRTLVIPPQLGYGARGAGGVIPPNATLIFDVELLDVQ"
     gene            18398..21067
                     /gene="pepN"
                     /locus_tag="VFO53_00215"
     CDS             18398..21067
                     /gene="pepN"
                     /locus_tag="VFO53_00215"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HET8874724.1"
                     /translation="MMSVLPRGAPPTPRRRADYRPPAFLIDDIALTFDLDPDATDVTA
                     TYAFRRNPAAAEVDRRAPLRLDGEQQARLHVALDARALPRERLLVDATSLTIADAPDA
                     GTLTIRTRIAPSRNVALEGLYVSSGVFCTQCEAEGFRRITYFLDRPDVLARYRVTLRA
                     ERTRYPVLLSNGNLVASGELADGRHFATWHDPFRKPTYLFALVAGDLASLDDAFTTRS
                     GRSVALRIYATPANVARCRHAMQSLQAAMRWDEERFGREYDLDTFMIFCADDFNLGAM
                     ENKGLNIFNSRLVLADPATATDADYCAIEGVIGHEYFHNWSGNRVTCRDWFQLSLKEG
                     LTVFRDQEFSSERGSRVVERIGAVEYLRREQFAEDAGPLAHPVRPDEYQEINNFYTAT
                     VYEKGAELIRMQHALLGAERFRRGMDLYFDRHDGKAVTCDDFVQAMQDASGVDLTQFR
                     RWYAQPGTPLVSVRGRYDAAARTYTLDIEQSVPSASAGGRVPPLHIPLALGLVGPGGS
                     DMPLRLSGEPTAGATTRLLELTRASHRFEFTDIDAEPVPSLFRGFSAPVQVACDHRDR
                     DLALLAMHDSDPVNRWDAAQRAFVDAILALAKAHRCGEPLALPALLHAIAGSLLDDEA
                     CDPALRALALALPDPAYVASLEPTLEPDGVMSAWTFVEKALANAHRGAFERAYERHRP
                     GVAYLPTQPQIAARSLSNQCLRYLGALDDDGAHALAARQYRQADNMTDTIGALAALRD
                     SASPVRDELYAAFERKWRDEPLVLDKWFALQAKSHRAEVLATVRALIAHPRFNARNPN
                     RVRSLVGAFALGNFAAFNARDGSGYAFAAAQVRSLDATNPQLASTIAGAFNLWKRFAE
                     PRRGTMLRCLSRIARTPHLSPDVSEVVTRMLAG"
     gene            complement(21068..21823)
                     /locus_tag="VFO53_00220"
     CDS             complement(21068..21823)
                     /locus_tag="VFO53_00220"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HET8874725.1"
                     /translation="MSAWIEGRVAGRREWSASLCSLLVDAPQLAFTAGQFARLSLPAP
                     AGSKEPMIGRPYSFVNPPSRQPHEFYFIILPEGPLSPRLARLGEGESVWLAPRANGFF
                     TIADTAEAQSLWCLSTGTGIGPFLSMLRTDDPWTKFERVVLVHSVRHADELTYRDEIA
                     GIARERSGAFGYIPMVSREAHSDALAGRIPSAIEDGRLEARAGAPLTAQSAHAMLCGN
                     PAMVDDVQKVLATRGMRRHRRREPGHVTLETYW"
     gene            21840..22451
                     /locus_tag="VFO53_00225"
     CDS             21840..22451
                     /locus_tag="VFO53_00225"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET8874726.1"
                     /translation="MTIMPTRKNPATLEGNRVHLRPPRRSDAHGFIAQAARSRALHHR
                     WVQPPDTPEAFAAYVQRYRRLDTGARHAGFVVLRNDDNALAGVVNFSEIVRGAFQSAY
                     VGYYAFAPLVGHGYMSEAFVLALDFAFGALELHRVEANVQPANSRSLALVERIGFARE
                     GYSRRYVKIAGRWRDHVRFAMLADEWARQRAAVRRRIAAAARD"
     gene            22444..22857
                     /locus_tag="VFO53_00230"
     CDS             22444..22857
                     /locus_tag="VFO53_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874727.1"
                     /translation="MIDPARDRAFAPLRFAAPCIAACISSCAAPAVAPGEPRIVERLV
                     IAPYDAHEQCAALAAGDRLDYRFESSMPLDFDIRYRQANAVLAPIVRGHSTSDSGIFE
                     AHEPARYCLDWQAGVDGAIIGYRIVVRRDAAYSSE"
     gene            complement(22842..23588)
                     /locus_tag="VFO53_00235"
     CDS             complement(22842..23588)
                     /locus_tag="VFO53_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8874728.1"
                     /translation="MSEADAGLQVIITGVSQGFGESLAFDLLGRGARVLGVGRSSSPR
                     LANPRYRFLRCDLAEAAMLPAMLEPAFAAIASQRPARVCLVNNAATLDPVGVLGTTEA
                     DHIMTSISVNLTAPVVLASVFCRVFADDARERRIINVSSGAAQSVIAGEALYCIAKAG
                     LEMLTRALAAEHGSQRFHAISLRPGVMDTQMQSYARTQPGERLPSVDLFKGFHARGQL
                     VAPDTVARKTVERLVLGDVEQGRTYSYSEL"
     gene            complement(23585..24694)
                     /locus_tag="VFO53_00240"
     CDS             complement(23585..24694)
                     /locus_tag="VFO53_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HET8874729.1"
                     /translation="MSAAASQTAETAQARFACPACGGEAVWDPARRKLVCPFCGTESP
                     ARIDATGAIVEHDLVAALRAIPDTARGWQLDAREVRCQSCNAISLLDPARQAQNCPFC
                     GSAQLVPYAQTKAAFRPEGVLPFSVPETAARDGIRAWYRRLWLAPSQLKRRALTDTVH
                     GVYLPYWTFDAHVEADWSAEAGHYYYETETVMVNGQPRTRQVQRIRWEPAAGHVAHFF
                     DDDLVCASTGVQPALIGGIEPFPTHELKPYDAAYVAGWVVERYQIDLVGAAQRARAAM
                     DAKLAALCASQVQGDTYRNLSVRPDYSRQTFKHVLAPVWLLTYTYGSRRFQCVMNGVT
                     GAVRGEYPKSPWKIALLALAIVVAVVIVMTLGGRQ"
     gene            complement(24691..25758)
                     /locus_tag="VFO53_00245"
     CDS             complement(24691..25758)
                     /locus_tag="VFO53_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH and helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET8874730.1"
                     /translation="MAILDFLGKQFIDIIEWTDDSRDTLSYRFPDEDKEIKNGAQLIV
                     RESQAAQFVYLGQFGDRFAPGRYTLTTDNIPILTDLKGWKYAFASPFKADVYFVVTRL
                     FTGNKWGTANPVMMRDADFGIVRLRAYGTYDFRIADVPKFLKEVAGTDDHFRLDEFND
                     TMRSRIVSVFSDALAQAKIPALDVAARYSELGEALLPVINPIIGDKYGLAMTSFVVEN
                     VSVPAEVEQAIDKRSSMAAVGNLNDYVKYQMGQGMEKGGGGVAGVGAEMAVGMAMAQQ
                     MVNPSGGLIAQATKGIEAAPASGNGNGVPQLMNPAQVAQMLGVSESDVLASLEAGDLK
                     GKKIGTQWRVPKAAVDAFLGS"
     gene            complement(25774..26604)
                     /locus_tag="VFO53_00250"
     CDS             complement(25774..26604)
                     /locus_tag="VFO53_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HET8874731.1"
                     /translation="MQRIENRRLQRAYRALAIGVVALACSGTAALAQNASAVPAAASA
                     DPAPASPDIAAIRSALGQRFPGAQINHVAKSSYFGLYEVMLDDNLVYTDASAAYIFVG
                     SMYDTATKQNLTEARSRKLNRVAVDKLPYELAFKRVKGDGSRKLVIFSDADCPFCHRL
                     ETEIKGLDNVTIYTFLFPIDQLHPNAAKKSKQIWCSADKAQAWDAFFASGKLPDNKGD
                     CGDPVAKTQALGQSLRINATPTLIFADGTMVPGALPLPQIEKEMTSAEAEKKKLAAGK
                     "
     gene            complement(26646..27854)
                     /locus_tag="VFO53_00255"
     CDS             complement(26646..27854)
                     /locus_tag="VFO53_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8874732.1"
                     /translation="MSGAPNAQVPGTGGADVLVAGAGLVGLALAAALARNGLSVTLLD
                     RVQPSATAAAADPGTWDQRVYAISPGSAAFLSRVGAWQALSCERIEAIESMRIVGDAG
                     ATLNFSAYEIGERALAWIVEERSLRAALLPAVHVPGIEIVSGAEFASLEWSTQEGALT
                     LADGRRYKASLIVGADGVQSWVRHAAGIVAEPKPYGQQGVVANFDCDLAHHGCARQWF
                     RDDGSVLAWLPLPGRRVSIVWSAPDALARELLALSPEALSERVADAGARVLGELRMIT
                     PPAAFPLSSLRLPTSIAHRTALVGDAAHGVHPLAGQGVNLGFGDAQALAGVLAERGPV
                     GDAGAPILLERFARRRAEPVLAMHAVTDGLVRLFGPRTPWLRTLRNAGLSAVDRLPMI
                     KRALAQPALR"
     gene            complement(27893..28432)
                     /gene="hslV"
                     /locus_tag="VFO53_00260"
     CDS             complement(27893..28432)
                     /gene="hslV"
                     /locus_tag="VFO53_00260"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HET8874733.1"
                     /translation="MAEVFHGTTILSVRRDAAVALGGDGQVTLGQVVIKSSARKVRRL
                     YHDKVIAGFAGATADAFTLFERFEAKLEKHQGQLTRAAVELAKDWRSDRVLRRLEAML
                     AIADRTASLVITGAGDVLEPEHGIVAIGSGGPYAQAAAKALLDNTSLPARAIVERALA
                     IAGEICIYTNQQIVIETLE"
     gene            complement(28444..29682)
                     /locus_tag="VFO53_00265"
     CDS             complement(28444..29682)
                     /locus_tag="VFO53_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET8874734.1"
                     /translation="MAIFGKPSTRKTASAKPAEGDAKRETKVMSARELAARAASGKGK
                     LPGLEPLGETSMRGASVINWSTAPKAIEVAQANPGLCAVLENAALLFANGQDEPARAL
                     LEQGVASDPDTKLSPLAWLALFDLMQRAGERAAFDQLAMQYVLQFERSAPSWDEAMKP
                     AGGEIAGGGGYIAITGKLSSRSESQLEGLKRALEKCVPRARIDLASVTGFDDAGARLL
                     AADLARARSQRMELAIERPARLRAALEEAVAKGRDGGEGAWLLSLELMQWAHEQAAFD
                     ERAVEFAVAFELSPPSWEPPPGGGRADAVEPAAANAVAHAAGAAETDHVVFDGVMTGA
                     SPAALARLADFAARNPVAPIDMTGVERIDFICAGALANAIGRIESQGKGVQIFGATPI
                     VRALLLLIGISPRHFVKKTG"
     gene            complement(29683..30279)
                     /locus_tag="VFO53_00270"
     CDS             complement(29683..30279)
                     /locus_tag="VFO53_00270"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012412690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET8874735.1"
                     /translation="MFTLPPLPYAENALEPVISANTLSFHYGKHHKAYVDNLNKLTSG
                     TEYESAPLEQVIRGTAGKADKSGIFNNAAQVWNHTFFWNCLRNGAGGKPTGRIAQMID
                     SDLGGYDNFRKEFANACVTQFGSGWGWLVANGGKLSIMKTPNAENPLSKGQTALLTID
                     VWEHAYYLDYQNRRPDFVNAVIDKLLNWDFATENLARA"
     gene            complement(30372..30818)
                     /gene="dksA"
                     /locus_tag="VFO53_00275"
     CDS             complement(30372..30818)
                     /gene="dksA"
                     /locus_tag="VFO53_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HET8874736.1"
                     /translation="MAAKTAMAVKKPPRDLTEEEILKAPEKDYMNEAQLAFFRKRLED
                     IRDQILRNADDTGEHLRENEITTDPSDRATLEEEYTLELRTRDRERKLLKKVEKSLRM
                     IEDGSYGYCEETGEPIGVPRLIARPMTTLSIEAQERRERVQKLYGD"
     gene            31018..32058
                     /gene="pyrC"
                     /locus_tag="VFO53_00280"
     CDS             31018..32058
                     /gene="pyrC"
                     /locus_tag="VFO53_00280"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET8874737.1"
                     /translation="MSPRRIRLRRPDDCHLHLRDGAALRAVLPATAQAFGRAIVMPNL
                     TPPVTTTAAAGAYRERILAARPAGSVFEPLMTLYLTDGTSAQEIARARASGFVHAVKY
                     YPAGATTNSRSGVTALANTYPALAAMEREGVVLAIHGEVTDPGVDVFDRERVFVEREL
                     SAIVRDFPGLRIVLEHATTSDAVAFVRAAPANVAATLTPQHLLYSRNALFAGGLRPHL
                     YCLPVLKRETHRRALVAAATSGSPKFFLGTDSAPHAKAAKETACGCAGCYSAPVALAL
                     YAEAFEDAGALDRLEGFASLHGAAFYGLAPNADAITLVRETSRVPDTLPFGDDRIVPL
                     GAGTDLRWRLLA"
     gene            32114..>35163
                     /locus_tag="VFO53_00285"
     CDS             32114..>35163
                     /locus_tag="VFO53_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET8874738.1"
                     /translation="MKAKTRGRGQAAPRRAADERNPPATALLVRARELAWTGEHERAI
                     DTCAEALATESSPEGRVDLLDLRAESYIASGHLDHAASDARTMVELAAASSSATLAAK
                     AGNRLALVQMRQGDMKRALATARRALDAARKGGDERSVAQGLLRLGEAQMRMRAGKAT
                     IATALEAAEHYRRLNDGSGEGRALWIVAHVLFAQNRREESRKAAERAIALCRQAGDRY
                     GVGNGLNALGITDSGVAENIRHLRQAAQEFEAAGYIDRGITIRTNLGLAYRELGLHRH
                     SFRLQNEVIEQSRSIGANVTALYGLASAIVEAVALGDLEWARNQLPLLVQGVPVLNDP
                     SMELAVFGCRGVLACADGDFASAAKHFRRGVEIARHVGSGSESRYLTLLGSAQRQAGK
                     IRAALAATSKATSIHRSLGYPKPEAFSAQEIWWRHAQALRAAGKSEAATKACARAYRL
                     LCDSIGTLHDEGLRRNYVGKVGANREIIASWVEEGTKRNLSKSELLAHLAIEADPREP
                     FQRLVDTGLRLNALRSTEEIRNFVVEEAVELCGGDRALLVLEEANRRTVGNAIVPRGE
                     DAEEVCRKINPFLDRASHTRGVELLHTPASARRIGQRSRIVAPLVVQNRLLGYLYADI
                     DGLYGRFNESDRDLLGMLANQAAVALDNARWAEGLERKVDERTAELQASNASLAQRNS
                     ELAIINSVQAALAAELDIQGIYDAVGDKIREIFGNRDLGIRIYDPKTNVVHYPYVYEG
                     GTRLEIASGPLAESGFGAHVIRTRATLVVNENMEQIAAKYGSFIIPGTTAEKSVVFVP
                     LVVSGEARGLINLNDVEHEHAFSESDVRLLQTLANSMSVALENARLFDETQRLLKETE
                     QRNAELAIITSVQAALAAELDIQGIYAAVGDKIGEIFAGSDVDIRIYDPATNLCHFPY
                     TRENGQRIKFAAQPLPEAGFGPHVIRTRETIVINERMAEAMAKYGSYLMPGSQLEKSS
                     VFVPITVGDQARGLIHLMDAEREHAFSDSDVRLLQTLANSMSVAL"
BASE COUNT         5869 a        12142 c        11696 g         5456 t
ORIGIN      
        1 ctgcctgcag ccgataaccg ggcgcgctgc tgaccgacgt gaggttgccg accttcagtg
       61 cgagaaacag gatcgccgcg atgccgatcg tcacgaagat gccgacccac aggtcgatcg
      121 tcgagcggct catcgcgtat aggcacggcc ggccggaccg tggcgtcgcg tgggcaaaga
      181 gatcatcagt gcactcccct gaacatgaac accgtgagca cgaaatcgag cgcgaggatg
      241 gacagcgagc tcgcgacgac ggtacgtgtg gttgcggtgg aaacgccttc ggccgtcggc
      301 cgcgcgttgt agccttcgaa caccgcgatc gcgctgaccg cgatcccgaa tacgacgctc
      361 ttgatgaagc cgttcacgat gtcgtagcgg aaatcgaccg cggcctgcat gttgccccag
      421 aacgagcccg agtcgatgcc gatgagccgg acgccgacga tgtaggcgcc gaagacgccg
      481 agcgtcgaga acagtgcggc gagcagcggc atggagacga cgccgccgac gaaccttggc
      541 gcaacgacgc gcgcgagcgg atcgacggcc atcatttcca tcgcggacag ttgctcggtg
      601 gtcttcatga ggccgatttc agcggtcacg gcactgcccg cgcggctcgc aaacagcaga
      661 ccggcgacga cggggccgag ttcccgcacg agcgacagcg cgacgaggat gccgagcgac
      721 tcgttcgcac cgtagcggga aagcacgtca tagccctgca gggcgagcac catgccgacg
      781 aaaagccccg acacgagaat gatcacgagc gacagcacgc cggagaaata gatctcgcgc
      841 agcgtgagtt gcaggcgtcg aaacgcgccg gggaggtgcc gcaggatcgc gatcgagaag
      901 cgcgcggcga acccgaggcg cgcaaccgaa tcgatcgtcg cggaaccgac gcggcggagc
      961 gcccaggcga gcgaatcgct cacgcctcgg cctcccgccg cgctcatgcg aactcctcct
     1021 tcaccgcctt cgccgggtaa tgaaacggca ccggcccgtc cggctcgccg tggacgaact
     1081 ggcgcacgaa cgcatcgctc gacgcgcgca cctcgtcggg cgtgccctgc gcgatgatgc
     1141 ggccttccga cacgaaatag atgtagtcga cgatcttgag cgactcgtag atgtcgtgcg
     1201 tcacgacgat cgacgtgatg ccgagcgcat cgttgagccg gcggatcagc tgtccgacga
     1261 tgccgagcga aatcggatcg agaccggcga acggctcgtc gtacatcacg agcatcggat
     1321 cgagcgcaat ggcgcgtgcg agcgcgacac ggcgcgccat gccgcccgac agctcggagg
     1381 ggcgaagctg cgccgcgccg cgcaggccga cggcgttgag cttcatcagc acgagatcgc
     1441 ggacgagctc ctcctgcagc tccgtgtgct cgcgcaacgg aaacgcgatg ttctcgaaca
     1501 ccgacatgtc ggtgaacagc gcgccgaact ggaacagcat gccgatcctg cgccgcagcg
     1561 cgaagagcgc gtcgcgatcg agcgaggcca cgtcctctcc ggcgacgctg atcgtgccgg
     1621 cctgcgggcg caactggccg ccgatgaggc gcaggatcgt cgtcttgccg cagcccgatc
     1681 cgcccatgat cgcgaccagc cgtccgcgcg ggatcgcgag gtcgatgccg cgcagcacgg
     1741 gccgcttcgg atcgtagccg aacgacacgc ccgtcaggcg gacggcgatt tcagtcgggc
     1801 acgtcggcga gacggcagcc ggttcggcaa cggacattgg gggacagccg gaaggacgga
     1861 acaggagatt gtagccggtt gccggctgcc gccctcgcat cccgaacccg gtgcaccgcg
     1921 cggcggatgc gggacggtat aatttgcacg atgatcgccg acgccgatgc gcgcagcgac
     1981 gctcgcgacg gcgccccacg caccgtcgac gccgagcgcg cactgaatct cgcccgcaac
     2041 gtgctcgaca ccgaggcgcg tgcgatctcg gcactttccg cgcgcctgtc ggcgccattc
     2101 gtcgccgccg tcgacgtcat gctcggctgc cgcggccgcg tcgtcgtgct cggcatcggc
     2161 aaatccggcc acatcgcacg caagctcgcc gcgacgctcg cgtccaccgg cacgcccgcg
     2221 ttcttcgtgc atccggccga ggccgggcac ggtgacctcg gcatgatcac cgccgacgac
     2281 gtcgtcgtga tgctgtccaa ttccggcgaa accgacgagc tgctgctcct cacgccgcac
     2341 ctcaagcgtc agggcgccaa gctggtcgcg ctcacgggca acgagaagag ctcgattgca
     2401 caatcggccg acgtgcacct cgacgccgcc gtcgatgcgg aggcgtgtcc gctcgggctc
     2461 gcaccgaccg caagcacgac ggcggcgctc gcgctcggcg atgcgcttgc gctcgcgctg
     2521 ctcgatgcgc gcggattctc ggtccacgac ttcgcgcgct cgcatccggg cggcgcactc
     2581 gggcggcggc ttttgacccg cgttgccgac gtgatgcgaa ccggcgcggc gttgccgcgc
     2641 gttgcgcata ccgcgacgct ggccgaggca atcgtcgaga tgaccgcgaa gggcctcggc
     2701 atgacggccg tcgtcgacgc gcggaaccgg atcatgggcg tcttcaccga cggcgatctg
     2761 cgccgctcgt tgacccgtgt cgtcgacgca acgaccgcgc gcgttgccga cctgatgacg
     2821 aagtcgccgc gaacgatcgg cgccgagcgc ctcgccgccg attgcatcgt gctcatggaa
     2881 acgccgccga agatcaccgt gctcctcgtg gccggggacg cgggcgagct cgtcggcgcc
     2941 gtgcagatgc acgacctctt ccgcgcacgc gtgctgtgat gcgagagcca gcgccaaccg
     3001 caagcgacat agcattgcgc gcgcgtcgcg tgcgcctttt gacctgcgac gtcgacggcg
     3061 tgctgaccga cgggcgcatc tacgtcgacg accagggacg cgagctgaag gcgttctcgg
     3121 cgctcgacgg cgtcgcgatg catcggctcg ccgctgcggg catcgtcgtc gcatggatca
     3181 ccggcagcgc cgcggcggcg gtaacgcatc gcgcgcagca gttgcgcatt ccgcatctcg
     3241 tgctcgacgc gcaggacaag ctcgtgccat gggagcgcct gcgcgccgag ctcggcctcg
     3301 ccgcggacgc atgcgcgcat atcggcgacg atctggcgga tattcccgtg ctcgccgcgt
     3361 gcggactcgg cgtcaccgtc ccgcatgcgc cttcggtagt ccgcgcgaac gcgcactacg
     3421 tgaccgtgcg cgacggcggc cacggcgcgg tacgcgagct cgccgacatg atcctggcgg
     3481 cgcagggcta cgatgacgcg gccggcgtcg cgccgccacg cgcatcggcg gcgcttgcgt
     3541 cgccggtgcg ctgacgagcc gcgtggcgat ggaccgcagc cgcgcactgt tcgatcgcct
     3601 cgtcgcgtgg tcgcccgtgc tgctgctcgg cgggctcgcc gcgctcacct actggctcga
     3661 cgcccaggtg cagacggcaa cgacgcaccg cgacggctcg acgcggcacg atcccgatct
     3721 gttcctgcgc gacttcaagg ccgtgacctt cgacgtccac ggattgccgc gcgaatcgct
     3781 gtccgcggcg agcgccgagc attttcccga caacgacagc gccgagctgc gcgcgccgcg
     3841 gctttcgctg accgagcccg ggcggccgac gatgacgatc accgccgagc gcggcgtgat
     3901 cgccggcgac cgcgagcatg gcgatttcat gggcgacgtg cgcgtcgagc gccaggccga
     3961 tccggcaccg tcgaagccag gcgacgacgc gagcgggccg gtgacgctca ccaccagcct
     4021 tctgcacgtt ttcacgaagg agcagcgcgt gacgaccgac cagccggtga cgatcgagga
     4081 gccgcgtgga ataatccgcg gccagggcct cgaattcgac aacaaggcga agcgcgtgcg
     4141 catcctgtcg catgtcagtg gaacgctgtc accgcaacga caaccgcccc gatgacccca
     4201 tacggccgtt ccagtgcacc ggtgcgcgcg acgctcgcat gcgtcgtcat gctgctcgcc
     4261 gcggctcctg tcgatgcgga gaaagccgat cgcgagcaga acatcaactt ctccgccgag
     4321 cagccggtcg aggtcgactt cgacaagcgc gtgggaacgc tgcgcgggaa cgtggtcatc
     4381 acgcagggca cgctgacgat ccgggccgac aagatcgact tcaagcagaa ttccgacgat
     4441 tccctatccg caaccgcgtg gggcaatccg atccgctttc gcgagaagaa ggacgattcc
     4501 gacgagtact acgaaggctt cgcgcagcgt gccgtctaca acgggcagac gcacacgctc
     4561 gagctgttcg acaacgcgct cctcaaggaa ggcaacaacg agtttcgcgg caactacatc
     4621 tcgtacaaca gcgacaccaa tttcatgaag gcggaaggcc ggcccaatgc gccgggtgcc
     4681 gaaggtcccg gcgcacgcgt gcaaggcgtg ttcgagccac gctccgaggg cccgccaggc
     4741 accaggggcg caccgaaggc gaacgaaaag acaccggacg gcacgaaagc cgcgccgaaa
     4801 gccgaagcca cgggagcgga cggcaggaag tccggcacga gcaagtcctc cgccggtgct
     4861 cgcaccgaag cgaaggccgc gccgccactt gcactgacgc ccgatccgac gcttcccgcg
     4921 aagtgagcac atccgcggcc gtcaacgcca acctttcggc agcgatggag cggcgccctt
     4981 cgcacgcgcc cggggatgca ccgagcaagc tcgcggcggc ccatctgcgc aagcgctaca
     5041 aggcgcgcac ggtggtccac gacgtttcgc tcgacgtcgc cagcggcgaa gtcgtcggac
     5101 tcctcggccc caatggcgcg ggcaagacga cgtgcttcta catgatcgtc ggcctcgtcg
     5161 ccgccgacgg cggcgacatt tcgctcgatg gcgagaacct gtcgcggctg cccatccacc
     5221 gccgcgcgcg gcgcggactt tcgtacctgc cgcaggaagc gtcgatcttt cgcaagatga
     5281 ccgtcgcgca gaacgtgcgc gcgatcctcg agctgcagga cctcgatccc gacaccctgt
     5341 cgaaccggct cgacgcgctg ctcgacgacc tcaccatctc ccacctcgcg gatgcgcccg
     5401 cggtctcgct gtccggcggc gagcggcgtc gcgtcgagat cgcccgcgcg ctcgcaacgc
     5461 gtccgcgctt catcctgctc gacgagccgt ttgcgggcgt cgatccgatc gcggttctcg
     5521 acatccagaa gatcatcggc ttcctcaagg aacgcggcat cggcgtgctg atcaccgacc
     5581 ataacgtgcg cgaaacgctc ggcatctgcg accgcgcgta catcatcaac gaagggcgcg
     5641 tcctcgcgtc gggcgcgccg ggcgagattg tttataatga ggatgtccgg aaagtttatc
     5701 tcggggagca cttccggctc tgactcgcgg tggcacgaac cgtcgtgctt cgtactcggt
     5761 ggatggcgtt ggtgctgatg cgcggtcgac cgattgcgca ccgtcgtttc cgggcgcaag
     5821 ctgcatcctt tcaagaaagc gcgctcgccg gtgaaacaga cgctccagct caagctctcg
     5881 cagcacctca cgctgacgcc gcaattgcag cagtcgatcc ggctgctgca attgtcgacg
     5941 atcgagctga atgccgaggt cgagcgaatg ctgcaggaga acccgctcct cgagcgcgcc
     6001 gaggtcgagg aagatcgcat cgcggccgaa gtgccgctgt cgggtcccgg tcgcgtgggc
     6061 gacggggtcg atcacgcgac ccatgacggc acggctggtg agtccgccgc cgagattcgc
     6121 tcgacgcgcg acgacgcgcc cgaggtcgtc gacttcgccg actacggcgg cagcagcggc
     6181 gacagcgact ggggtggcag caattcgaac gacgacgacg acttcctgcc gcagcagatc
     6241 gccaccagca cgttgcgcga tcatctgaac ggccagcttg cgctgctgaa cctgccgctg
     6301 cgcgaccggc acatcgtcac cgcgctgatc gacgcgctcg acgacgacgg ctatctcgcg
     6361 accccgctcg acgagatcgc cgagctgttc cccgcggagc tcgagatcga tgccgacgac
     6421 ctcaacatag cgctcaagta cgtgcagagc ttcgagccgt cgggcgtcgg tgcgcgcgat
     6481 tgcgccgaat gcctcgcgct gcagcttcgt gcgctgccgg cggcaacgcc gtttctcgcc
     6541 gacgcgctga aggtcgtcga cggcaacctc gcgctgctcg ccaaccgcga tttcacgaag
     6601 ctcaagcggc ttttgcacac cgacgacacc ggcgttcgcg gcgtgcgcga cctcatccgc
     6661 agcctcaacc cccgcccggg cgcggcgttc gccaaggccg aggcgaacta cgtgatcccc
     6721 gatgtggtcg tacgcaagat caggaaccaa tgggtggcct cgctgaacga ggcggcgatg
     6781 ccgaagctgc ggctcaaccg catctatgcc gacatcctca cgcgcaaccg cgaaagctcc
     6841 aaccagcaac tggcggcgca gctgcaggaa gccaagtggc tgatccgcaa cgtgcagcag
     6901 cgtttcgaca cgatcctgcg cgtggcgaag gccatcgtcg agcggcagcg ccgcttcttc
     6961 gagcacggcg aggtcgcgat gcggccgatg gtgctgcgcg aaatcgccga catgctgtcg
     7021 ctgcatgaat ccacgatctc ccgcgtcacc acgcagaagt acatgctgac cccgcgcggc
     7081 acgttcgagc tcaagtattt cttcggcagc cacgtggcca cggacaccgg aggcgcggca
     7141 tcggccacgg cgatccgcgc gctgatcaag cagctgatcg ccgccgagga tgcgaagaca
     7201 ccgctcaccg acagcaagat cgccgacatg ctgggtgagc agggcatcct cgtcgcgcgg
     7261 cgcacgatcg ccaagtaccg cgaggcgctg cagataccgc cggtgaacat gcgcaaggcg
     7321 ctctagcagg aaaggcccca tgaatctgca cctcaccgga caccacgtcg acatcacgcc
     7381 ggcgatccgc gactacgtca tcgcaaagct caaccgcatc aatcgccatt tcgatcatgt
     7441 catcgacgtg agcgtggtgc tcaccgtcga caggctttcg cacaaggtgg aagccacgct
     7501 gcacgtgcgc ggcaaggacc ttcacgccga gtgcatcgat gccgacatgt acgcggcgat
     7561 cgacggcctc gccgacaagc tcgaccgcca ggtgctgaaa tacaaggagc agatcaagga
     7621 ccatcgcacc gacaccgagg cgatcaaccg gtcggcggcc gaaaccggca ccgagggcac
     7681 atcgtagcgg gctgtaacgg gggcagcggc agtgcctggc ttgccggggc gctgaacgta
     7741 taatcggccg gtttgccctc gagcgctcga cgcgcggccg gacctcgtga atcagatcgc
     7801 ggcactgctg cctgcgtcca acatcctcgt cgacctcgac gtcgcctcga aggcagccct
     7861 cttcggcacc atgggcgagg tgttcgagcg cagcgcggga ctgcctcgcg cgggcgtcgc
     7921 ggcaagcctc gccgcgcgcg agaagctcgg gtccaccggg ctcggccagg gcatcgccat
     7981 tccgcacggg cgcattgcgc ggctgtccgc cgcaatcggc gcattcgcgc ggcttcgcgc
     8041 tccgatcgcc ttcgatgcgc ccgacgggaa accggtcgac caggcgttcg tgctgctcgt
     8101 tcccgagcag gccaccgatc agcacttgca gctcctgtcc gagctcgcgc agatgttttc
     8161 cgagcgcgcg ttccgcgacc ggctgatggc cgcgaaggac gccgccgcgc ttgccggcat
     8221 ctttcgcggc tggcagccgc aggactgacg taccgacgac cgatgcgcca ggtcagcgtc
     8281 gaacgcttcc tcgaggacaa tcgcgagcgc ctcgggctcg tgtggcttgc cggccgcgac
     8341 ggcggcgacc ggatgctggt cggcgaaagc gccttgcgcc cgacgatcgg ccagatcggc
     8401 cacatgaacc tgatccatcc gttccgcgtg cagatcctcg gcgcggccga gctcgcctac
     8461 ctgcgcgcgc tgcccgagga cgagctcaac cggtcgatca accggatgtt cacgaccgag
     8521 ctcgcggcca tcgtcgtcgg caacggcgag caggttccac ggcatctgct cgacatgtgc
     8581 aaccagcacc gcatcgcgct cgtcacgtcg cagcagccgt cgccgcatat cgtcgacgtg
     8641 ttccgcatct acctttcgcg cgtgctcgcc gagtcgacga cgctgcacgg cgtgttcctc
     8701 gacgtgctcg agctcggcgt gctgatcacc ggcgcgtcgg cgatcggcaa gagcgaattg
     8761 gcgctggagc tcatctcgcg cggcaacgga ctcatcgccg acgacatcgt cgagctccac
     8821 cggatctcgc ccgacacgat cgagggccgc tgtcccgccg tgctgcgcga ttttctcgag
     8881 gtgcgcggca tcggcatcct caacatacgc acgatcttcg gcgaaaccgc ggtgcgcccg
     8941 cacaagctgc tgcggctcat cgtgcatctc gaggatcacg gcaacgaggc gttctccgac
     9001 ctcgaccggc tgcaggtcaa cgccacgttc caggaagtgc tgtcggtgcc gatccgcaag
     9061 gtgaccatcc cggtcgctgc cgggcgcaat ctcgcggtgc tggtcgaggc ggccgtgcgc
     9121 aacttcgtgc tgaaccagcg cggcatcgac agcacgaagg aattcatcga gcggcagcag
     9181 aagctgatgg acgagggctg agttggacct catgctgatc ggcggcgtct cggggtcggg
     9241 caagagcgtg gcacttgccg cattcgagga cgccggctac tacgccgtca acaacctgcc
     9301 cgtgccgctg ctcttcgaca cggcgagcta tctcgaaggc tccggacagg aacgcgtagc
     9361 gattgcgctc gacgtgaaga gcggtcccgg actgcccggg ctcgcggagg cgatggcggc
     9421 gctcgagcag cgcggctgga aggttcgcct cctctatctc gacgccaccg tcgaaacgct
     9481 cgtgaagcgc ttctccgaga cgcggcggcg ccatccgttc tcgagcggcg atcgcacgct
     9541 gaccgaggcg atcgagtacg agcgcgcgtt gctcgctcat gcacgccagc tcggcacgac
     9601 gttcgacacg tcggagctgc cggccgcgca cctgcgcgga tggatcaagg atttcgtttc
     9661 cgtcgacgca tcgcagatca cgctgcagct cgagtcgttc ggcttcaagc acggcgtgcc
     9721 actcgacgcc gacctggtgt tcgacgcgcg gtgcctgccc aatccgcact acgagccggc
     9781 gctgtcgccg ctcaccggca agcaccccgg cgtcgtcgcg ttcctcgagg cgataccgga
     9841 ggtcgagcgc atgtacggcg acatctacca tttcatcgcg agctggctgc ccgactacgc
     9901 acgcgacaac cgcaactacc tgacggtggc catcggctgc accggcggac agcatcgctc
     9961 ggtgtacatg gtcgagcggc ttctccaggc gttttcgccg cgctaccagg tgctcgtccg
    10021 gcatcgcgag ctcgcacccg gcgacgcgcc agcgacggat ggcgcatctt cacccgcaac
    10081 gccgggatga gcgagcccgc cgcgcgcgcc tcgtcgtcgc tgccgctttt tccgctgcgc
    10141 acggttctct tccccggtgg cctgctgccg ctcaaggtgt tcgagcagcg ctacatcgag
    10201 atgacgaagg cgtgtcttcg cgacgagcgg ccgttcggcg tgtgcctcat cacgcacggc
    10261 gacgaagtcg cgcgcggcga cggcgcgttt ccggagatcg cgaaagtcgg cacgcttgcc
    10321 cgcatcacga gtgtcgacat gccgcagatg ggcatcctgc acctgtccac cgcgggcggc
    10381 gcgcgcttcg aagtgcgcag ccacgcgccc gatgcgctcg gcatcctggt cgcgagcgtg
    10441 acgccgatcg acgacgagcc gcggcatccg ctcgccgaaa cgtatgcgcc gctcgcacgg
    10501 ctgctcgagg tgatcgcaac gcggcttggc gccagcaact tccccgccgc cacgcattac
    10561 gacgatgcgt cgtgggtcgg ctaccggctg gcggagctgc tgccactgcc gctgtcgatc
    10621 aagcagagca tgctcgaggt cagcgacgcc aatgtgcgtc tcgccgcatt gaagcagttc
    10681 ctcgagcgcc aggggctgtt gcaggccgta taatttgccc tctcgcgcgc aatcgatgca
    10741 tcgggcatgg ccggtcacag caagtgggcg aacatcaagc acaagaaggc cgccgccgat
    10801 gcgaagcgcg gcaaggtgtt cacgcgcctc atcaaggaaa tcaccgtcgc cgcgcgcctc
    10861 ggcggcggcg atgcatcggc caatccgcgc cttcgtcttg cgatggacaa ggccatgggc
    10921 cagaacatgc cgaaggacac gatcgagcgc gcgatcaagc gcggcgcggg cggcctcgaa
    10981 ggcgtcaact acgaagagat ccgctacgag ggctacggca tcggcggcgc cgcagtgatc
    11041 gtcgactgca tgaccgacaa ccgcacgcgc acggtggccg acgtgcgcca cgcgttctcc
    11101 aaatacggcg gcaacctcgg caccgacggc tcggtcgcgt tcctgtttcg ccacgtcggc
    11161 cagatcctct ttgcacccgg cagtgacgaa gacaaggtga tggaagcggc catcgacgcc
    11221 ggcgccgacg acgtggtcac gcacgaggac ggcagcatcg aggtgctcac cgcgccgaac
    11281 gacttcatcg cggtgaagga tgcgctcgcg aagtccgggc tcgcgcccga gttcgccgag
    11341 ctgacgatga aaccgctgac cgaggtcgac atgcgcggtg acgatgcgca gcggatgcag
    11401 aagctcctcg acgcgctcga atcgctcgac gacgtgcagg acgtgtacac gtcggcggcg
    11461 atcgaggaag cctagcgggc gtcacgagcc acggccccag gtgcccgcca tcggcgtccg
    11521 ccgcatactc ggcatcgatc ccggcttgcg cgtcacgggc ttcggcattc tcgaagcgcg
    11581 cggcgccacg ctcgtctacg tcagcagcgg ctgcattcgc gcaacgggaa gcagcctgcc
    11641 gatccgcctc ggcgtcatcg tgcgcgacct cgcgcacgtg atcgcggagg agcggccaac
    11701 ggaagtcgcc gtcgagaaag tgttcgtgaa catcaatccg aactcgacgc tgctgctcgg
    11761 ccaggcgcgc ggggcggcga ttgccgccgc ggtcatcgcc ggcctgccgg tcagcgaata
    11821 cacggcggga caggtgaagc aggcggtcgt cggcgcgggg cgggcgttga agccgcaggt
    11881 gcaggagatg gtgcgccggc tgctgtcgct gcccggcatt cccgcatccg acgcggccga
    11941 cgcgcttgcc gccgccatct gccatgcaca cgcatcgacc ggcgtcggcg cactcgcgcg
    12001 gcatggctat cgcgttcgtg gcggacggct gctcgttaca tgaaggtttc gtccgcgtag
    12061 aattgcgccc catgatcggc cgtctctcgg gcacgctgct cgaaaagaat ccaccgcagc
    12121 tgttgctcga cgtgcagggg gtggcctacg aggtggacgt gccgatgagc acgttctaca
    12181 acctgccggc gaacggcgag cgcgtgacgc tgttcacgca tctcgtcgtg cgcgaggacg
    12241 cgcacctgct ctacggattc ggcagcgaca acgagcggcg cgcattccgt cagctgctga
    12301 aggtcaacgg catcggcgcg aagatcgcgc tgtcgctgct gtcgggactg tcggtcaccg
    12361 agctcgcaca ggcaatcgcg atgcaggaga cgggacggct gacgcgcgtg ccggggatcg
    12421 gcaggaaaac cgccgagcgg ctcctgctcg agctcaagga caagctcggc accgagctca
    12481 cgcacgcgat cggcgtcaac cgggcgccgc cggcaacgtc ggatatcatg catgcgctca
    12541 ttgcgctcgg ttacagcgag cgcgaagcgg ccgcagcgat caggcagctt ccggacggcg
    12601 tcgcggtggc cgacggaatc aagcaggcgc tgaagctgct cgccaaaccg tgacgccgcg
    12661 ctgcttgcct cgcgcgtgtg aatcggcctg cggccgcgca tggcgcaaac cggaccctgg
    12721 atggcgatcg aaaacgacga actgacccgc atcgttgcaa cgaacagcac cggtacgcag
    12781 gaagaggcgc tcgaacgggc gctgcgcccg cgccggcttg ccgagtacat cgggcaggaa
    12841 aagattcgcg accagctcgg catcttcatc gaagccgcgc gaaggcgcag cgaagcgctc
    12901 gatcacgtgc tgctgttcgg ccccccgggt ctcggcaaga cgacgctgtc gcacatcctc
    12961 gcgcacgagc tcggcgtgaa tctgcgccag acgtcgggac ccgtgctcga gcggcccggc
    13021 gaccttgccg ccatcctcac gaacctcgag ccgcgcgacg tgctgttcat cgacgagatc
    13081 caccggctgt cgccgatcgt cgaggagatc ctctatccgg cgctcgagga tttccagatc
    13141 gacatcatga tcggcgaagg tcccgccgca cgcagcgtga agatcgacct gccgccgttc
    13201 acgctggtcg gcgccacgac gcgtgcgggg atgctcacca atccgctgcg cgatcgcttc
    13261 ggcatcgtcg cgcggctcga gttctattcg ccgcaggagc tgacgcagat cgtcgcgcgg
    13321 tcggcgcggc tcctgtcgat gctcgtggag cccgacggcg cgctcgaaat tgcccgccgc
    13381 gcacgcggca cgccgcgcat cgcgaaccgc ctgctgcgac gcgtgcgcga ttacgccgag
    13441 gtgcgcgccg gcggcgacgt cacgcgccac gtcgccgacg ccgcgctgtc gatgctcgac
    13501 gtcgacgcat cgggcctcga cacgatggac cgcaagctgc tgtcgaccgt gctcgacaag
    13561 ttcggagggg gaccggtcgg cgtcgacaac cttgccgcgg caatcggcga ggagcgcgac
    13621 acgatcgagg acgtgctcga gccctacctg atccagcagg gcttcctgca gcgcacgtcg
    13681 cgcgggcgcg tcgccacgcc gctcgcgtat cggcatttcg gattggcggc accggcggcg
    13741 ggcggcgacc tgtttcccgg ccagtgacgc tttcgcgcgt actgcccgcc gttacgagcg
    13801 tcaaagggaa cggcgcgccc ccaaccagag aggccacgca cgcgtgaacg tccacaccga
    13861 cctttcgttc ctgacgctga tcatccacgc gtccgtcatc gtccagggga tcatcgcgct
    13921 gctcgcgctg ctctcgatgt ggtcgtggtg gcagatcttc ctcaagatgt tccagctgcg
    13981 gcgcatcgcg cgggagaccg acgcgttcga ggacgagttc tggcagggcg gcaacctcga
    14041 aacgctctac aggcaggcgc gcgaggcgca ggtcggcggc ggactcgagc agatcttcac
    14101 cgcgggcttt cgcgaattct ccaagcatcg gcgccaggga agcgccgccg acgtcacgct
    14161 cgacgcagcg cggcgggcga tgcgcgcgac ctaccagcgc gaaatcgacg cgatcgaggc
    14221 gcacctcgcc gggctcgcca ccgtcggttc ggtttcgccc tacatcgggc tcttcggcac
    14281 cgtgtggggc atcatgaatg cctttcgcgg cctcgccaat gtcagccagg cgaccctcgc
    14341 aaacgttgca ccgggtattg ccgaagcatt gatcgcaacg gcaatcggcc tgtttgccgc
    14401 gatcccggcc gtcgtcgcct acaaccgctt cacgcacgac gtcgaccggc tggccgtgcg
    14461 cttcgagacg ttcatcgagg agttttcgaa catcctgcaa cgccaggggt aacgctcgca
    14521 ccgattcccg atcaccgatc cccgattccc gatttccgat ccccgacaca tgcgacgcgc
    14581 aaggaagccg atcaaccaga tcaacgtggt gccgtacatc gacgtgatgc tggtgctgct
    14641 ggtgatcttc atggtcaccg cgccgctcgt ggcgccgggc gagatccagt tgccctcggt
    14701 cgggtcgaag ctcaccaatc ccgacaaccc gttgaacgtg agcctgaaag gcacgacgat
    14761 cacgctgacc gacaagcttg cgaagaccga gcggagggtc tcgaaggacc agctcgtcgc
    14821 ggacgtcctc gccgcgcagg ccggacagga gcgcccggtg gtaatcgcag ccgacaagga
    14881 tgcacgctac caggacgtgc tcgacatcct cgatatcctg cagcgcagcg gcgtgaaaag
    14941 ggtcggactg ctcgcgcgtc cgccagccgc ctgatggcga ccgtcatgtc tccgcacgag
    15001 cggccggcgc cggcgcagcg cgcgcgcggc aaatggacgg cgctgatcct tgcgctgttc
    15061 gtcaacctgc tcttcgtcgg cgtgctcgtg ttctcggtgt cctggcagaa ccgcccgccg
    15121 caggcggtga cggccgagct ctatgcgccg ccgccgccaa ccgtcaccgc gccgcggcgt
    15181 gtcgagccga agccgcaacc gaagctcgag ccgatcccgc cgaagccgga gccactgccg
    15241 ccaaagctcg agccgatgcc gaagccgccc gagccggccg tcgcgaaacc ggacacgcag
    15301 gcagccgaca tcgcgctcaa ggccaggcgc gaagcggagc gcatccgcca ggagcaggtc
    15361 gagcgcgaaa accaccgaca ggaagaggca cgccgacagg aggaagcgga aaagcgcgac
    15421 gaggcccgca ggcaggccgc ggcgcgtcag gccgccgagg acaagcggca ggccgagttg
    15481 cgcgagcgcc agcagcgcca gctcgagacg atgcgcgcgc aggcggcgca ggaagcgcag
    15541 atgcgcacgc aggcggaacg cgaatcgcag atgcgcgcac aggccgagcg cgaatcgcgc
    15601 ctgcgtgcgg aggccgagcg cgaagcgcgc gggcgcgccg agcaggcggc ggccggtgcg
    15661 cgcaacaagg cgcagctcga ctggatcgac aagattcgca gccgcattcg cggcaacatc
    15721 aatcttccgc ccgacattcc cggcaacccg gaggcggtgt tcgacgtcgt gcagttgccc
    15781 accggcgaga tcatcgacgt gaagctgcgc aaatcgagcg gcgttcgcgc ctacgacgaa
    15841 gcagtgcagc gcgcgatact caaatcatcg ccgctgccca agccggtgcc cgccgacctg
    15901 tttcagcgca gcctcgagct gcgcttccgt cctctcgatc agtagcggcg cttcattcgg
    15961 cgcccgtgcg gggctgtttc tcgacgatct ggatcgcggt ccagctccag tcgccgcgct
    16021 gctcgacgag ctggcgcagc gccaccgtat agaccgagcg cccggtgatc agaatcagct
    16081 tgcgcccttc cgcatattcg tgcgtgggca cgatcagcgt cttgacgacg agcggcttgt
    16141 cgggacgcga cggcggcggc aggtaaaggc cttcgaagcg cggtcccatc atcgacgtgt
    16201 cgagtccgtt gacgacgatg ccgagctcct gcttgccctc gcgcttcgcg tagagcgtga
    16261 cggcgacgac gcgctcggca atgagcgaaa cgcccgcctc gacgtcctcg accgagacct
    16321 tgttgagccg gcgcacgacg ccgagcaacc aatgcgaggc atccgactgc cgcacggcga
    16381 cgagcgcacc gagcatcagc gtctgcccta cgccgccgct tgcgcaaatg cgcaacccgg
    16441 cgagactgcg atccttcacc tgccacaccg gatcggagaa cgtggcaagg ctcgacgaca
    16501 gcgtgtcggc ttcgtcgacg agctgcggct tcgtgcgcgc cggccccgtg actgcgtaga
    16561 cctcgatctc ctcgccgctg gtggtgtcgc cgaccggcgc gtccttctgc ggcttgttca
    16621 gctcgtgctg gatgcgcgcg agcccgacgc gcacacgcgc cgcgacgacg cacggcgtgc
    16681 gcggatcgcg gcgcatgtcg gtgttgagat tgggcgcgat cgcggcgcgc gccttctcga
    16741 ggatcatcgc gcgctgctgg ttgatcgggc ccaccggccc ctggtcggtt tcctcggagt
    16801 gacgaagcgc cgcgagagac atgtcgagct ggctggcgag cagcgtcgtg tcgagatagc
    16861 gcagcatcga ccccgaatcc tggccggtgc ggcgcgtgag gccggtgcgg cccgcgacgt
    16921 cgacgaaaaa gccttccatc gtcttcggca gcgcaacgag ctcgagcttg cggctgaagc
    16981 cgcgcacctg cgacgcggcc cagtcgagat tggccggcga aaggttgccg ctgttcaact
    17041 ggtgcagcag cagcgcatag acgtattcct gctcgaccgt ccactgcgtg ccgttgccgc
    17101 ccgacgcgcc gagcgaggtc ggcacgcggt cgacgccaag ctccgtcgcg cgcatgtagg
    17161 cgcgatgcag ctcgagccat ttcgcgggaa tccagtgctc gtggcggcat acgcgcagct
    17221 tcgcgtcggt gccgtagtaa tggacgagcc gtgcgaagag gatcggcacg tggggcttcc
    17281 agcggccgta cgcggcgggc gccagcgcct cctcgagcgt gcgctggtat gcggcgatga
    17341 acgcctgcgc gagatcgagg ctcgcctgcc agatgcgctc ggaaaccttc ggcgccgatt
    17401 cgaggctctc gacgtattgg cgaaaaagct ggcgccggtc ggcgcccagc gcggcgtcga
    17461 tgtattgcag cgcctgcgca cgcgcgaggt cgaccgggcg acgcgactgg cgcatgccgt
    17521 cgaacgcgcg cacgacgagc tgctgccgcg cgacggcatc gagcgacggc aactggcgca
    17581 gccaggtggc ggccgacttg accgagacga tgggatcggc cgtgtcgcgc ggcgccgatg
    17641 acaggaatgc gaacatggcg cccattcacg accctcgcaa cgaatcatgc gcacgctgca
    17701 cgcaatggtc gcgcattgtg caaagcggat ctggcggtgt acgaagcatc gatgtcctcg
    17761 ttcgagcgac gcgcagcggg acgcgcacag cgggtcggat aaaatgcagt ggcgccgcag
    17821 tccggcgcgt cgcgccgcga aaccggcggc gcctttccca acgaggtcct cgcatgcccc
    17881 tccccgttcg tcgtttctca cgctcgctgt gcggcacctg cgcacactgg gcgctgattg
    17941 cgctcatccc gatggcgatc ggatgcaccg cgacggccgc cactgcgccg acctctgcca
    18001 gcgaaggaca aagcaagttg aatgccacac cggagaacct gcagaaaatc gatgtgaaac
    18061 agggcacggg caccgaagcc gttgcgggtc acaccgttgt agtgcactac acaggctggc
    18121 tgtacgacag ttcgaagccc gattcgcatg gtgcgaagtt cgacagctcg cgcgaccgca
    18181 acgcaccctt cagcttcgcg ctcggcgccg gccgcgtgat caagggctgg gaccagggcg
    18241 ttgcaggcat gaaggtgggc gggcagcgca cgctcgtgat tccgccgcaa ctcggctacg
    18301 gtgcgcgcgg tgccggcggc gtcattccac cgaacgcgac gctgatcttc gacgtcgaac
    18361 tgctcgacgt acagtagacg cacggccgat tgccgcgatg atgtccgtgc ttccgcgcgg
    18421 ggcgccgccg acgccgcggc gccgagccga ttatcgtccg ccggcgttcc tgatcgatga
    18481 catcgcgctc acgttcgatc tcgatcccga cgcgaccgac gtcaccgcga cgtacgcgtt
    18541 tcgccgcaat ccggccgcgg ccgaagtcga tcggcgtgca ccgctcaggc tcgacggcga
    18601 gcagcaggcg cggctgcatg tggcgctcga cgcccgcgcg ctgccgcgcg agcgtctgct
    18661 cgtcgacgcg acgtcgctca cgatcgccga tgcgcccgac gccggcacgc tcacgatccg
    18721 cacgcgcatc gcgccgtcgc gcaatgtcgc gctcgaaggc ctgtacgtct cgtcgggcgt
    18781 gttctgcacg cagtgcgagg ccgagggctt tcgccggatc acctacttcc tcgaccggcc
    18841 cgacgtcctc gcgcgctacc gcgtgacgct gcgtgccgaa cgcacgcgct atccggtgct
    18901 gctttcgaac ggcaacctcg tcgcgtcggg cgagcttgcc gatggccgtc acttcgccac
    18961 gtggcacgat ccgtttcgga agccgaccta cctctttgcc ctcgtcgccg gcgacctcgc
    19021 gtcgctcgac gatgcgttca cgacgcggtc gggccgaagc gtcgcgctgc gcatttacgc
    19081 gacgcccgcc aatgtcgcgc gctgccggca tgcgatgcaa tcgctgcagg ccgcaatgcg
    19141 ctgggacgag gagcgcttcg gccgcgaata cgatctcgac acgttcatga tcttctgcgc
    19201 cgacgacttc aacctcggcg cgatggagaa caagggcctc aacatcttca acagccggct
    19261 cgtgctcgcc gatcccgcga cggcgaccga cgccgactac tgcgccatcg agggcgtgat
    19321 cggccacgag tatttccaca actggagcgg caaccgcgtc acgtgccgcg actggttcca
    19381 gctctcgctg aaggaagggc tcacggtttt tcgcgaccag gagttctcga gcgagcgcgg
    19441 ctcgcgcgtc gtcgagcgca tcggcgccgt cgagtacctg cgtcgcgagc agttcgccga
    19501 ggatgccggc ccgctggcgc atccggtgcg tccggacgag taccaggaga tcaacaactt
    19561 ctacacggcg accgtttacg aaaagggcgc cgagctgatc cggatgcagc acgcgctgct
    19621 cggggccgag cgctttcgtc gcggcatgga cctctatttc gaccgtcacg acggcaaggc
    19681 ggtcacctgc gacgatttcg tgcaggcgat gcaggatgcg tcgggcgtgg acctcacgca
    19741 attccggcgc tggtacgcgc agccggggac gccgctcgtc agcgtacgcg gccgctacga
    19801 tgccgccgcg cgcacctaca cgctcgacat cgagcaaagc gttccgtcgg caagcgccgg
    19861 cggccgcgtg ccgccgctgc acattccgct tgcgctcggt ctcgtcggcc ccggcggcag
    19921 cgacatgccg ttgcgcctgt cgggggagcc gacggcgggt gcgacgacgc gtcttctcga
    19981 gcttacccgg gcgtcacacc ggttcgagtt caccgacatc gacgcggaac cggtgccgtc
    20041 gctgttccgc ggcttttccg ccccggtgca agtggcctgc gaccaccgcg accgcgacct
    20101 cgcactcctc gccatgcacg acagcgatcc cgtgaaccgc tgggacgccg cgcagcgtgc
    20161 attcgtcgat gcgatcctgg cacttgcgaa ggcgcaccgg tgcggcgagc cgctcgcgct
    20221 gccggcactg ctgcacgcga tcgccggttc cctcctcgac gacgaggcgt gcgacccggc
    20281 gttgcgtgcg ctcgcgctgg cactccccga tcccgcgtac gtcgcatcgc tcgagcccac
    20341 gctcgagccc gacggcgtga tgagcgcgtg gacgttcgtc gagaaggcgc ttgcgaacgc
    20401 gcatcgaggc gcgttcgagc gcgcctatga aaggcatcgg ccaggcgtcg cgtatctgcc
    20461 gacgcagcca cagatcgccg cgcgcagtct ttcgaaccag tgcctgcgct atctgggcgc
    20521 gctcgacgac gacggcgccc acgcgctcgc ggctcggcaa taccggcagg ccgacaacat
    20581 gaccgatacg atcggcgccc tcgcggcgct gcgcgattcg gcctcgcccg tgcgcgacga
    20641 gctgtacgcc gcgttcgaga ggaagtggcg cgatgagccg ctcgtgctcg acaagtggtt
    20701 cgccctgcag gcaaaaagcc accgtgcaga ggtgctggcc acggtgcgtg cgctgatcgc
    20761 gcatccccgc ttcaatgcgc gcaatcccaa ccgcgtgcgc tcgctggtcg gggcattcgc
    20821 gctcggcaac ttcgcggcgt tcaacgcgcg cgacggctcg ggatatgcgt tcgccgccgc
    20881 acaggtgcgc tcgctcgacg ccacgaatcc gcagctcgcc tcgacgatcg ccggcgcgtt
    20941 caatctgtgg aagcgcttcg ccgagcctcg ccggggaacg atgctgcgct gcctgtcgcg
    21001 catcgcgcgc acgccgcacc tgtcgcccga cgtgagcgag gtggtgacgc ggatgctcgc
    21061 gggctgatca ccagtacgtc tccagcgtga catgccccgg ctcgcggcgg cgatgccggc
    21121 gcatgccgcg cgtcgccagc accttctgca cgtcgtccac catcgccggg ttgccgcaca
    21181 gcatcgcgtg cgcgctttgc gcggtgagcg gcgcgccggc gcgcgcctcg agccgcccgt
    21241 cttcgatggc gctcggaatg cgccccgcga gcgcatcgga atgcgcctcg cggctcacca
    21301 tcggaatgta gccgaacgcg ccggagcgct cgcgcgcgat gccggcgatc tcgtcgcggt
    21361 acgtaagctc gtcggcgtgg cgcaccgaat gcacgagcac gacgcgctcg aacttcgtcc
    21421 acggatcgtc ggtgcgaagc atcgacagga aggggccgat gcccgttccc gtcgacaggc
    21481 accaaagcga ttgcgcctcg gccgtatcgg cgatcgtgaa aaagccgttg gcgcgcggcg
    21541 caagccacac gctctcgcct tcgccgagcc gcgcaagccg cggtgacagc gggccttcgg
    21601 gaaggatgat gaagtagaac tcgtgcggct ggcgcgacgg cggattcacg aacgagtagg
    21661 gccggccgat catcggctcc ttcgatcccg ccggcgccgg aagcgacagc cgcgcgaatt
    21721 gtccggcggt gaacgcgagc tgcggcgcgt cgacgagcag cgaacagagg cttgcgctcc
    21781 actcacggcg acccgcgaca cggccttcga tccacgcgct caatcgacct cccgctaaaa
    21841 tgacgattat gcctacgcgc aagaacccgg cgacgctcga aggcaatcgg gttcacctgc
    21901 ggccgccgcg gcgaagcgac gcgcacgggt tcatcgcaca ggcggcgcga agccgcgcgc
    21961 tgcatcatcg ctgggtgcag cctcccgaca cgccggaggc gttcgccgcc tatgtgcagc
    22021 gctaccggag gctcgacacg ggcgcgcggc atgccggctt cgtggttctg cgcaatgacg
    22081 acaacgcgct cgccggcgtc gtcaacttct cggagatcgt gcgcggcgca ttccagagcg
    22141 cgtacgtcgg ctactacgcg ttcgccccgc tcgtcggtca cggctacatg agcgaagcgt
    22201 tcgtgctcgc gctcgatttc gcgttcggcg cgctcgagct ccaccgcgtc gaggcgaacg
    22261 tgcagccggc gaacagccgc tcgcttgcac tcgtggagcg catcggcttc gcgcgcgagg
    22321 gttactcgcg gcgttacgtg aagatcgccg gccgctggcg cgaccacgtg cgctttgcga
    22381 tgctggcgga cgaatgggcg cgacagcgtg cggccgtgcg ccggcgcatc gcggccgccg
    22441 cgcgtgattg acccggctcg cgatcgcgca ttcgcgccgc ttcgcttcgc ggcgccgtgc
    22501 atcgctgcgt gcatttcatc ctgcgcggcg cccgcggttg caccgggcga gccgcggatc
    22561 gtcgagcgac tggtcatcgc gccgtacgat gcgcacgagc agtgcgcagc actcgccgcc
    22621 ggcgatcggc tcgattaccg cttcgagagc agcatgccgc tcgacttcga catccgctac
    22681 cggcaggcga acgccgtgct ggcgccgatc gtgcgcggcc attcgacgtc ggacagtggc
    22741 atcttcgagg cgcacgagcc ggcgcgctac tgcctcgact ggcaggcggg cgtcgacggc
    22801 gcgatcatcg gctaccggat cgtcgttcgg cgcgacgccg cttacagctc cgaatagctg
    22861 taggtgcggc cctgctcgac gtcgcccagc accagccgct cgacggtctt tcgcgccacg
    22921 gtatcgggtg ctacgagctg tccgcgcgca tggaaaccct tgaacagatc cacgctcggc
    22981 agccgctcac cgggctgcgt gcgcgcatac gactgcatct gcgtatccat cacgccgggg
    23041 cgcaggctga tcgcatggaa tcgctgcgac ccgtgctccg ccgcgagcgc gcgggtgagc
    23101 atctcgaggc cggccttcgc tatgcaatac agcgcctcgc cggcgatcac gctttgcgcg
    23161 gccccggacg agacgttgat gatgcgccgc tcgcgcgcat cgtcggcgaa tacgcgacag
    23221 aaaacgctcg cgagcacgac cggcgcggtg aggttgacgc tgatcgacgt catgatgtga
    23281 tcggcctccg tcgtcccgag cacgccgacc ggatcgagcg tggcggcgtt gttgacgagg
    23341 cacacgcgcg cgggccgctg cgatgcgatg gccgcgaacg cgggctccag catcgcggga
    23401 agcatcgccg cttcggcgag gtcgcagcgc aggaagcgat agcgcgggtt cgcaagacgc
    23461 ggcgacgacg agcggccgac gccgagcacc cgcgcgccgc gcccgaggag gtcgaacgcc
    23521 agcgactcgc cgaatccctg cgacacgccg gtgatgatca cctgcagccc tgcgtcagct
    23581 tcgctcattg ccgtcctccg agtgtcatga cgatcacgac cgcaacgacg atcgcgagcg
    23641 cgagcagcgc gatcttccac gggctcttcg gatactcgcc gcgcacggcg cccgtcacgc
    23701 cattcatcac gcactggaag cggcgtgaac cgtacgtgta cgtcagcagc cagacgggtg
    23761 ccaggacgtg cttgaacgtc tgccgcgagt aatcggggcg caccgacagg tttcgatacg
    23821 tatcgccctg cacctgcgac gcgcaaaggg cggcgagctt cgcgtccatc gccgcgcgtg
    23881 cacgctgtgc cgcaccgacg aggtcgatct ggtagcgctc cacgacccat cctgcgacgt
    23941 atgcagcgtc gtagggcttg agctcatggg tcgggaacgg ctcgatcccg ccgatcagcg
    24001 cgggctgcac gccggtggat gcgcagacga gatcgtcatc gaagaaatgc gcgacgtgtc
    24061 ccgccgcagg ctcccagcgg atgcgctgga cctggcgcgt gcgcggctgg ccgttcacca
    24121 tcacggtttc ggtctcgtag taataatggc cggcctcggc gctccagtcg gcctcgacgt
    24181 gggcgtcgaa cgtccaatag ggcagataca cgccgtgcac ggtatcggtc agcgcacgcc
    24241 gcttgagctg cgaaggcgcc agccagaggc gcctgtacca cgcgcggatg ccgtcgcgcg
    24301 cggcggtttc cggaaccgag aacggcagca cgccctcggg gcggaacgcg gccttcgtct
    24361 gcgcataagg cacgagctgc gccgagccgc agaacgggca gttctgcgcc tgccgcgcgg
    24421 gatcgagcag cgagatcgcg ttgcagctct ggcagcgcac ctcgcgcgcg tcgagctgcc
    24481 atccgcgcgc cgtgtcggga atcgcgcgca gcgcggcaac gagatcgtgc tcgacgatgg
    24541 caccggtcgc atcgatgcgc gccggcgact cggtgccgca gaacgggcac acgagcttgc
    24601 gccgcgccgg atcccagacg gcttcgccgc cgcaggcggg acaggcgaac ctggcttgcg
    24661 cggtctccgc ggtctgtgac gcggccgcgc tcacgaaccg aggaacgcgt cgaccgccgc
    24721 cttgggcacg cgccattgcg tgccgatctt cttgcccttg aggtcgccgg cttcgaggct
    24781 cgcgagaacg tcggattccg acacgccgag catctgcgcc acctgcgccg gattcatcag
    24841 ctgcggcacg ccgttaccgt tgccgctcgc cggcgccgcc tcgatgccct tcgtcgcctg
    24901 cgcgatgagg ccgcccgacg ggttgaccat ctgctgcgcc atcgccatgc cgacggccat
    24961 ttcggcaccg acgccggcga caccgccgcc gcccttctcc atcccctgcc ccatctggta
    25021 cttcacgtag tcgttcaggt tgccgacggc ggccatgctc gagcgcttgt cgatcgcctg
    25081 ctcgacctcg gcgggcaccg agacgttctc gacgacgaac gacgtcatcg cgaggccgta
    25141 cttgtcgccg atgatcgggt tgatcacggg cagcagcgcc tcgccgagct ccgagtagcg
    25201 tgcggcgacg tcgagcgcgg gaatcttcgc ctgcgcgagc gcatcggaga acacgctgac
    25261 gatgcgcgag cgcatggtgt cgttgaattc gtccagccgg aaatgatcgt cggtgcctgc
    25321 gacttccttc aggaacttcg gcacgtcggc aatgcgaaag tcgtacgtgc cgtacgcgcg
    25381 aagccgcacg atgccgaagt ccgcgtcgcg catcatcacc ggattggccg tgccccactt
    25441 gttgccggtg aaaagccgcg tgacgacgaa gtagacgtcg gccttgaacg ggcttgcgaa
    25501 cgcgtacttc cagcccttga gatcggtcag gatcgggatg ttgtccgtcg tgagcgtgta
    25561 gcgcccgggc gcgaagcgat cgccgaactg gccgagatag acgaactgcg ccgcctgcga
    25621 ttcgcgcacg atcagctgcg cgccgttctt gatctccttg tcctcgtcgg ggaagcgata
    25681 cgacagcgtg tcgcgcgagt cgtccgtcca ctcgatgatg tcgatgaact gcttgccgag
    25741 gaaatcgagg atcgccatgg cgcttctttc gcgttacttc ccggccgcga gcttcttttt
    25801 ctcggcctcg gcgctcgtca tttccttctc gatctgcggc agcggcagcg cgccggggac
    25861 catcgtcccg tcggcaaaga tcagcgtcgg ggtcgcgttg atgcgcagcg actgcccgag
    25921 cgcctgcgtc ttcgcgaccg gatcgccgca atcgcccttg ttgtcgggca gcttgcccga
    25981 cgcgaagaac gcgtcccacg cctgcgcctt gtcggccgag caccagatct gcttcgactt
    26041 cttcgccgca ttcggatgca actggtcgat cgggaacagg aacgtgtaga tcgtcacgtt
    26101 gtcgaggccc ttgatctcgg tttcgagacg atggcagaac gggcagtcgg catcggagaa
    26161 gatcacgagc ttgcgcgacc cgtcgccctt gacgcgcttg aacgcgagct cgtacggcag
    26221 cttgtcgacg gcgacgcgat tgagcttgcg cgaccgcgcc tcggtcaggt tctgcttcgt
    26281 cgccgtgtcg tacatcgagc cgacgaagat gtaggcggcc gacgcatcgg tgtagacgag
    26341 gttgtcgtcg agcatcactt catagaggcc gaagtagctc gacttcgcga cgtggttgat
    26401 ctgcgcgccg ggaaagcgct gcccgagcgc gctgcggatg gccgcgatgt ccgggcttgc
    26461 cggggccggg tcggcgctcg cggccgccgg cacggcggac gcgttctgcg cgagcgcggc
    26521 cgtacccgag caggcgagtg cgacgacgcc gatggcaagc gcgcggtacg cgcgttgcaa
    26581 ccggcggttc tcgattcgtt gcatgaaatt ctccaatgtg actgcggcga agcagttggg
    26641 gacagctagc gcaacgcagg ctgggccagc gcgcgcttga tcatcggcaa acggtcgacc
    26701 gccgacaggc ccgcattgcg cagtgtccgc agccacggcg tgcgcgggcc gaacaggcgc
    26761 acgaggccat cggtcacggc gtgcattgcg agcaccggct cggcgcgccg gcgcgcgaag
    26821 cgttcgagca ggatcggcgc gccggcatcg ccgacggggc cgcgctcggc cagtaccccg
    26881 gcaagcgcct gcgcgtcccc gaagccgaga ttaacaccct gtccggccag cggatgcacg
    26941 ccgtgggcag catcgccgac aagcgccgtc cgatgggcga tggacgtggg caggcgcagc
    27001 gaggagagcg gaaaggcggc cggcggcgtg atcatccgca attcgccaag caccctggcg
    27061 ccggcgtcgg cgacccgctc cgatagcgcc tcgggcgaca gggccagcag ctcgcgcgcg
    27121 agcgcgtcgg gtgccgacca cacgatcgag acccggcgtc cgggcagcgg aagccacgcg
    27181 agcacgctgc catcgtcgcg gaaccactgc cgcgcgcatc catggtgcgc gagatcgcag
    27241 tcgaagttcg cgaccacgcc ctgctgaccg tacggtttcg gttcggcgac gatgcccgcg
    27301 gcgtgccgca cccacgactg cacgccgtcg gcgccgacga tcaggctcgc cttgtagcgg
    27361 cgcccgtcgg cgagcgtcag cgcgccctcc tgtgtcgacc attcgagcga cgcgaattcg
    27421 gcccccgaga cgatctcgat gcccggcacg tgcacggcgg gcaacagcgc tgcacgcaac
    27481 gaccgctcct cgacgatcca ggcgagcgcg cgctcaccga tctcgtaggc cgaaaagttg
    27541 agcgtcgcgc ccgcgtcgcc gacgatgcgc atcgactcga tggcctcgat gcgctcgcac
    27601 gacaacgcct gccacgcgcc gacgcgcgaa aggaacgcgg cgctgccggg gctgatcgca
    27661 tagacgcgct ggtcccaggt gccggggtcg gcggcggcgg cggtggcgga cggctgcacg
    27721 cggtcgagca gcgtgacgct gaggccgttt cgcgcgagtg ctgcggccag ggcgagtccg
    27781 acgaggcctg cgccggcgac gaggacatcg gcgccgccgg tacccggcac ctgcgcgtta
    27841 ggcgctccgc tcatgggaaa atccgccggc ggcggcgcgg cgctcgccat ggtcactcga
    27901 gcgtctcgat gacgatctgc tggttcgtat agatgcagat ctcgccggcg atggcgagcg
    27961 cacgctcgac gatcgcgcgc gccggcagtg acgtgttgtc gagcagcgcc ttcgcggccg
    28021 cctgcgcgta gggcccgccc gagccgatcg ccacgatgcc gtgctcgggc tcgagcacgt
    28081 cgcccgcgcc cgtgatgacg agcgacgcgg tgcgatcggc gatcgcgagc atcgcctcga
    28141 gcctgcgcag cacgcggtcg gagcgccagt ccttcgcgag ctcgaccgcc gctcgcgtca
    28201 gctgaccctg gtgcttttcg agcttggcct cgaagcgctc gaacaacgtg aacgcatcgg
    28261 ccgtggcacc ggcgaatccg gcgatcacct tgtcgtggta gagccttcgc accttgcgcg
    28321 cgctcgactt gataacaacc tggccgagcg tcacctggcc gtcgccgccg agcgccacgg
    28381 cagcgtcgcg gcgcaccgac aggatcgtcg ttccgtgaaa gacctcggcc atcgcggtgc
    28441 gtgtcagccc gtcttcttga cgaagtgccg cggtgaaatg ccgatcagca gcaacagcgc
    28501 gcgcacgatc ggcgtcgcgc cgaagatctg cacgcccttg ccctggctct cgatgcggcc
    28561 gatcgcgttg gcaagcgcgc ccgcgcagat gaaatcgatg cgctcgacgc cggtcatgtc
    28621 gatcggggcg accgggttgc gcgcggcgaa atcggcgagc cgagcgagcg ccgccggcga
    28681 cgcaccggtc atcacgccgt cgaacacgac atgatcggtc tcggccgctc cggcggcatg
    28741 cgcgacggca ttcgccgctg ccggctcgac tgcatcggcg cgtccgccgc ccggcggcgg
    28801 ttcccacgac ggcggcgaca attcgaacgc aaccgcgaat tcaaccgcgc gctcgtcgaa
    28861 cgcggcctgc tcgtgcgccc actgcatcag ctcgagcgaa agcagccacg cgccttcacc
    28921 gccgtcgcga ccctttgcga cggcttcctc gagcgccgcg cgcagcctcg cgggccgctc
    28981 gatggcgagc tccatgcgct ggcttcgcgc acgtgcaaga tcggcagcaa gcagccgcgc
    29041 cccggcatcg tcgaagccgg tgaccgaggc caggtcgatg cgtgcacgcg gaacgcattt
    29101 ctccagcgcg cgcttcagtc cctcgagctg cgattcgctg cggctcgaaa gctttccggt
    29161 gatggcgatg tagccgccgc cgcctgcaat ttcgccgccg gcgggcttca tcgcctcgtc
    29221 ccacgacggc gcggagcgct cgaactgcag cacgtactgc atggcgagct gatcgaacgc
    29281 cgcacgctcg cccgcgcgct gcatgaggtc gaagagcgca agccaggcga gcggcgagag
    29341 cttcgtgtcg gggtcgcttg ccacgccctg ctcgagcagc gcgcgcgccg gctcgtcctg
    29401 tccgttcgcg aacagcaagg ccgcgttctc gagcaccgcg cacaggcccg ggttggcctg
    29461 cgcgacctcg atcgccttcg gcgccgtcga ccagttgatg acgctcgcgc cacgcatgct
    29521 cgtttcgccg agcggctcga ggccgggcag cttgcccttt ccgctcgcgg cgcgcgccgc
    29581 gagctcgcgc gccgacatca ccttcgtctc gcgcttcgcg tccccctcgg ccggcttcgc
    29641 ggacgccgtc ttcctggtcg acggcttgcc aaaaatcgcc atttacgcgc gggcgaggtt
    29701 ttccgtcgcg aaatcccagt tcagcagctt gtcgatcacg gcgttcacga aatcgggacg
    29761 ccggttctgg tagtcgaggt aatacgcgtg ctcccagacg tcgatcgtca ggagcgccgt
    29821 ctggcccttc gacaacggat tttccgcgtt cggcgttttc atgatcgaaa gcttcccgcc
    29881 gttggccacg agccagcccc atcccgagcc gaactgggtg acgcaggcgt tcgcgaactc
    29941 tttcctgaaa ttgtcgtagc cgccgagatc gctgtcgatc atctgcgcga tgcgcccggt
    30001 gggcttgccg cccgcgccgt tgcgcaggca attccagaag aacgtatggt tccacacctg
    30061 ggcggcgttg ttgaagatgc cgctcttgtc cgccttgccc gccgtgccgc gaatcacctg
    30121 ctcgagcggt gcggactcgt attcggttcc gctcgtgagc ttgttcaggt tgtcgacgta
    30181 cgccttgtgg tgcttgccgt aatgaaacga gagcgtgttg gccgagatga ccggctcgag
    30241 cgcgttctcg gcgtacggaa gaggaggcag cgtgaacatg gggtcgggct ccttcgatcg
    30301 gtgaaaggga atgtcaaaaa ggggacgggc cgcatgcgcg gcccattccc agcgatcgaa
    30361 cgccggacgc gtcagtcgcc gtagagcttc tgcacccgtt cgcggcgctc ctgcgcctcg
    30421 atcgacagcg tggtcatcgg ccgcgcgatg agtcgcggca cgccgatcgg ctcgccggtt
    30481 tcctcgcaat agccatagct gccgtcctcg atcatgcgca aggacttttc gaccttcttg
    30541 agcagcttgc gttcccggtc gcgcgtgcgc agctcgagcg tgtattcctc ctcgagtgtt
    30601 gcacggtcgg agggatcggt cgtgatctcg ttctcgcgca gatgctcgcc ggtatcatcg
    30661 gcgttgcgca ggatctgatc gcgaatgtcc tccagccgct tcctgaagaa ggcgagttgc
    30721 gcctcgttca tgtagtcctt ctccggcgcc ttgaggatct cttcctcggt gaggtcgcgc
    30781 ggtggttttt tcactgccat tgcagttttg gcggccatgc ttcgttccat ccttgaactt
    30841 cgtaccggtg cgacgtcagt cattgcaatc ccctcatggg caaatcgaga ggcgcccgca
    30901 ctaaattgcg cattatagcc cgacgagccg ccgcaagcga atccgctcca gcatctgcgg
    30961 cggacgaccg gcgaatgcgc gggcgcccct acaatggttg aaaaacaaag gcttccgatg
    31021 tcacctcgcc gcatccggct ccgccgcccc gacgactgcc acctgcatct tcgcgatggc
    31081 gcagcccttc gtgccgtgct gcccgcaacg gctcaggcct tcggcagagc aatcgtcatg
    31141 ccgaatctca cgcctccggt gacgacgacg gcggccgccg gcgcgtaccg cgagcgcatc
    31201 ctcgctgcac ggcccgcggg cagcgtgttc gagccgctga tgacgctcta tctgaccgac
    31261 ggaacgtcgg cgcaggaaat agcgcgtgcg agggcgagcg gcttcgtgca cgcggtgaag
    31321 tactaccctg cgggcgcaac gacgaattcc cggtcgggcg tgacggcgct tgcgaatacg
    31381 tatccggcgc tcgccgcgat ggagcgcgaa ggtgtcgtgc tcgcgattca cggcgaagtc
    31441 accgaccccg gcgtcgacgt gttcgaccgc gagcgcgtgt tcgtcgagcg cgagctttcg
    31501 gcgatcgtgc gcgacttccc ggggcttcgc atcgtgctcg agcacgccac gacgagcgac
    31561 gccgtcgcat tcgttcgcgc cgcacccgcc aacgttgccg cgacgctcac gccgcagcat
    31621 ctgctgtact cacgcaatgc gctgttcgcc ggcggcctgc gtccgcacct ctattgcctg
    31681 ccagtgctca agcgcgagac gcaccggcgc gcgctcgttg cggcggccac gtcggggagc
    31741 ccgaaattct tcctcggcac cgattcggcg ccgcacgcga aagcagccaa ggagaccgcc
    31801 tgcggctgtg ccggctgcta ttcggcgccc gtggccctcg cgctctatgc ggaagccttc
    31861 gaagatgcgg gcgcgctcga ccggctcgaa ggcttcgcga gcctgcacgg tgccgcgttc
    31921 tacggtctcg ccccgaatgc cgatgcgatc acgctggtcc gcgagacgtc gcgcgtcccg
    31981 gacacgctcc cgttcggcga cgaccggatt gtgccgctgg gggcaggcac cgacctgcgt
    32041 tggcgattgc tcgcttgacc ccgcccggaa tccggcagca ccatcgccat tcgatcgagc
    32101 tcggaaggca gcgatgaagg cgaagacccg aggccgtggc caagccgcgc cgcgacgcgc
    32161 ggccgacgaa cgcaatccgc cggccactgc gctgctcgtc cgtgcgcgcg aacttgcgtg
    32221 gacgggcgag cacgaacgcg cgatcgatac gtgcgccgag gcactcgcca ccgaaagctc
    32281 accggaaggg cgcgtggacc ttctcgatct tcgcgccgaa tcgtacatcg cttcggggca
    32341 cctcgatcac gcggccagcg atgcgcggac gatggtcgag ctcgcagcgg cgtccagttc
    32401 cgcgacgctt gcggcgaagg cggggaatcg cctggcgctc gtgcagatgc ggcagggcga
    32461 catgaagcgc gcgttggcga cggcgcggcg ggcgctcgac gcggcgcgca agggcggtga
    32521 cgagcgtagc gtcgcccaag gcctgctccg gctcggtgaa gcgcagatgc gcatgcgtgc
    32581 cggcaaggcg accatcgcga ccgcgctcga agcggccgag cattatcgcc ggctcaatga
    32641 cggctcgggc gaaggccgcg cgctctggat cgtggcgcac gtgttgtttg cgcagaatcg
    32701 ccgggaagaa tcgcgcaagg ctgccgagcg cgccatcgcg ctctgccgcc aggccggcga
    32761 tcgctatggc gtcggcaacg ggctgaacgc gcttggtatc acggactcgg gtgtcgcgga
    32821 aaacattcga catctgcgcc aagccgcgca ggaattcgag gccgcaggtt acatcgaccg
    32881 cggcatcacg atccgcacga acctcgggtt ggcgtaccgg gaactgggac tgcaccggca
    32941 ctcgtttcgc ctgcagaacg aagtgatcga gcagagtcga agcatcggcg ccaacgtcac
    33001 ggcgctgtac gggctcgcga gtgcgatcgt ggaggcggtc gcgctcgggg atctcgagtg
    33061 ggcgcgaaac cagctgccgc tgctggtcca gggcgtaccg gtgctcaacg accccagcat
    33121 ggagctcgcc gtcttcggat gccgtggcgt gctcgcctgc gctgacggcg atttcgcgtc
    33181 ggcagcgaag cattttcgcc gaggcgtcga gatcgcgcgc catgtcggca gcggcagcga
    33241 gagccgatac ctgacgttgc tgggttcggc acagcgacag gccgggaaga tcagggcggc
    33301 gctcgccgcc acgtcgaagg ccacctcgat ccaccgctcg ctcggctacc cgaaacccga
    33361 ggccttcagc gcgcaggaga tctggtggcg ccacgcccag gcactacgcg cggctggcaa
    33421 gagcgaggct gcgacgaaag cgtgtgcgcg cgcgtaccgc ctgctgtgcg acagcatcgg
    33481 cacgttgcac gacgaagggc tgcgccggaa ctacgtcggc aaggttggcg caaaccgcga
    33541 aatcatcgcg agctgggtcg aggaaggcac gaagcgaaac ctttcgaaaa gcgagctcct
    33601 cgcccacctc gcgatcgagg ccgatccccg cgagccgttc cagcgcctgg tcgacacggg
    33661 gctgcgtctc aacgcgctgc gctcgaccga ggagatccgc aatttcgtcg tcgaggaggc
    33721 cgtcgagctt tgcggcggcg accgggcgct gttggtcctc gaagaggcca accggcgcac
    33781 cgtgggaaac gccatcgtgc cgcgtggcga agatgccgag gaggtgtgcc gcaagatcaa
    33841 tccgttcctc gatcgcgcga gccatacgcg cggcgtcgag ctattgcaca cgcccgcctc
    33901 ggctcgccgc atcgggcagc gctcgcgcat cgtcgcgccg ctggtcgttc agaaccgcct
    33961 gctcggctac ctttatgccg acatcgatgg cctttacggc cgcttcaacg aaagcgaccg
    34021 tgacctgctc ggcatgctcg cgaaccaggc ggcggttgcg ctcgataacg cgcgctgggc
    34081 cgaggggctc gaacgcaagg tcgacgagcg caccgcggaa ctgcaggcgt ccaacgcgag
    34141 cctcgcccag cgcaacagcg agctcgcgat catcaatagc gtgcaggcgg cgctcgcggc
    34201 cgagctcgat atccagggca tctacgacgc ggtcggcgac aagatccgcg aaattttcgg
    34261 caaccgcgat ctgggcatcc ggatctacga cccgaagacg aatgtcgtgc actatcccta
    34321 cgtgtacgaa ggcggcacgc ggctcgagat cgcgtcgggc ccgctggccg aatccggctt
    34381 cggcgcgcac gtgatccgta cgcgcgcgac gctggtcgtc aacgagaaca tggagcagat
    34441 cgccgcaaag tacggcagct tcatcattcc cggaaccacg gccgaaaaat cggtcgtgtt
    34501 cgtcccgctc gtcgtcagcg gcgaggcgcg cggcctcatc aacctgaacg acgtcgagca
    34561 cgagcatgcg ttcagcgagt ccgacgtgcg gctgctgcaa acgctcgcca acagcatgag
    34621 cgtcgcgctc gagaacgcgc ggctcttcga cgagacgcag cgcctgctca aggagaccga
    34681 gcagcgcaac gccgagctcg cgatcatcac cagcgtccag gcggcgctcg cggccgaact
    34741 cgacatccag ggcatctatg ccgcggtcgg cgacaagatc ggcgaaatct tcgcgggctc
    34801 cgacgtcgac atccgcatct acgatccggc gacgaacctg tgccatttcc catacacgcg
    34861 ggaaaatgga cagcgcatca agttcgccgc gcagccgctt ccggaagcgg ggttcgggcc
    34921 acatgtcatc cgcacgcgcg agacgatcgt gatcaacgag cgcatggcgg aagccatggc
    34981 gaagtacggc tcttatctga tgccgggctc gcaactggag aaatcgtcgg tgttcgtccc
    35041 gatcacggtc ggcgaccagg cgcgggggct cattcacctg atggacgcgg aacgcgagca
    35101 cgcgttctcc gactccgacg tgcgcctgct gcagacgctc gccaacagca tgagcgtcgc
    35161 gct
//
LOCUS       DASSVX010000005        15623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1449954, whole genome shotgun sequence.
ACCESSION   DASSVX010000005 DASSVX010000000
VERSION     DASSVX010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 15623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15623
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449954"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VFO53_00290"
     CDS             complement(<1..924)
                     /locus_tag="VFO53_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET8874739.1"
                     /translation="MAKIIGIDLGTTNSCVAIMEGGAPKVIENSEGGRTTPSVVAYAE
                     DGEILVGAPAKRQAVTNAKNTIFAVKRLIGRRFEEKEVQKDINLMPYRIVKADNNDAW
                     VEVRGKKIAPQQVSAEILRKMKKTAEDYLGEEVTDAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGLDKKEGDRKIAVYDLGGGTFDISIIEIANVEGEHQFEV
                     LSTNGDTFLGGEDFDQRIIEYVVNEFRKEAGVDLKNDVLALQRLKEAAEKAKVELSSS
                     QQTDINLPYITADQSGPKHLSMKLTRAKLESL"
     gene            complement(1008..1553)
                     /gene="grpE"
                     /locus_tag="VFO53_00295"
     CDS             complement(1008..1553)
                     /gene="grpE"
                     /locus_tag="VFO53_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET8874740.1"
                     /translation="MTDQERRTGFDHIDDNASDAAGPQDAVSGEDALAKARAEAADMK
                     DAWLRARAEVENVRRQGQADVARAHKYALEKFAGDLLAVKDALEQTLATDQSVAAETL
                     KAGSDLTLKALNAAFGRAAIREINPAGEKFDPHVHQAMQVVDSDLPPNTVVTVFQKGY
                     LLNDRVLRPALVTVSKAQQGS"
     gene            complement(1689..2798)
                     /gene="hemH"
                     /locus_tag="VFO53_00300"
     CDS             complement(1689..2798)
                     /gene="hemH"
                     /locus_tag="VFO53_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET8874741.1"
                     /translation="MAFRPEPPFAHDRTPKVGVLLVNLGTPDAPTASAVRRYLAEFLA
                     DPRVVEIPPLAWRPLLHGFVLRTRPARSAARYAAIWTKDGSPLGVHANKQKVLLSGSL
                     GQRLKALGLAADAVVVEQAMRYGAPPIGAGLERLRGAGCERILVVPMYPQYASSTTGS
                     VVDAVATHLAHTRRVPGLRFVDTFHDDPGYIAALAATVNDHWMKHGQPEHLVLSFHGL
                     PRRALARGDPYHCYCQATGRLLARELGLETSQWTLAFQSRFGRGRWLEPYTAATLAKL
                     GKARLRRVDVFAPGFVADCLETLEELAIEGKRVYRAAGGGDYDVIACLNEHPRWIAAL
                     TEIVLANLHGWLAPPPGIAEREATRLRAKALGAAG"
     gene            complement(2803..3822)
                     /locus_tag="VFO53_00305"
     CDS             complement(2803..3822)
                     /locus_tag="VFO53_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874742.1"
                     /translation="MTAGGRSPFVAIAFALPALTAERAYRALARRINGAWPLRTINRR
                     RLDHFHRTNASSGGHFYVIVMPGTLHFLLPCLRLLPSQQTVHLIANGARAWERRRLVA
                     AFPARPIVTLAALPGSSLAHGDVITLLLERNAAPFGLLDHDCYVFDATCFEGATPAPA
                     ECLTGWFRSVGRKSGLAYAETFFLILNAPLLRDIMRRHRVDARVYRRVPARLRALVAR
                     IGLRDGVAFKDHHDYLDTLHLLVALALAEGGTLGFRGSAGGAVVHLGGTSAGAPETKD
                     LLEHYLQLRFLELANDRELRARYRRRFRTFASSDDVRARIPMTPAAFARVGAIDRLVE
                     RLRDA"
     gene            complement(3962..4990)
                     /gene="hrcA"
                     /locus_tag="VFO53_00310"
     CDS             complement(3962..4990)
                     /gene="hrcA"
                     /locus_tag="VFO53_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004533556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET8874743.1"
                     /translation="MLDARAQHLLKILVERYVAEGQPVGSRALSRHSGLDLSPATIRN
                     VMADLEEIGLIASPHTSAGRVPTPRGYRFFVDSLMVVQPLEDVEIHRLEGELNVDRPQ
                     QIVNAAASLLSQLTQFAGVVATPKRREASFRHLEFLRLSERRVLLIIVTAEGDVQNRI
                     LHTDRSYTPSQLVEATNFFNQNFAGHPFTGIRDRLADELRALRDDIASLMKAAVDAGE
                     GALSQADALVVTGERNLLNAEDLASNMQRLRRLFDLFEQKTSLLHLLDISQRAPGVQI
                     YIGGESGLVPLDECSVVTASYEANGQVIGTLGVIGPTRMAYERVIPIVDMTARLLSNA
                     LSRELLDD"
     gene            5120..6031
                     /locus_tag="VFO53_00315"
     CDS             5120..6031
                     /locus_tag="VFO53_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET8874744.1"
                     /translation="MSARTPERFHRIALIGRHGGPAAATPVATLAALLVARGHTVVLE
                     PDAAGGLVLPGVTTLSDDALPDGVDLAIVLGGDGTMLSVARRLAPHDVPLIGVNLGRL
                     GFLTDIPLARMEPTIAAMLDGRYTEERRTLLSVTLCRADGTSTSAPALNEVVVNRGGL
                     GSMIECAVEVDGRFVYAMRADGVIVATPTGSTAYALSTGGPIIAPEVPAFALVPIAPH
                     ALTHRPIALPDRSTITITVSHGRDAAVHCDGQAHFELAEGDRVTARRAPHTARLLHPE
                     YHDYFAMLRAKLHWSETPERLTQGDAR"
     gene            6360..8030
                     /gene="recN"
                     /locus_tag="VFO53_00320"
     CDS             6360..8030
                     /gene="recN"
                     /locus_tag="VFO53_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET8874745.1"
                     /translation="MLSALSIRHFVVVDTLDVEFAPGFTVLTGETGAGKSILLDALGL
                     LLGDRFDTRQVRSGAERAELAAVFAIGGSSSVKNWLNAEGLDAGTELLLRRTLDVQGR
                     SRAWINGRPATLAQLTELGAQLLDLHGQHAHQALIGAEAQRTLLDEFGGFASSAADVA
                     DAWRAWRVAVERSEAAADADAAFAAERDALRERESELAELALAPDEWNALIATERRLV
                     NAGALIEVATQAEDALSEGDDAIGRRLARIAQKLAQASADDEALAPIVALLEPAAIQL
                     DEAARAVREYRRRLDLDPDELTRVQERLSAIHDVARKHRVRPDALPELALATRARLVE
                     LAQATDATALAEAASEARRRYDALARTLTQQRAGAAATLAQRVTAAMQELAMAGGRFD
                     VALEALPLPASSGRESVAFRIATHPKQPPLPLSRVASGGELSRLALAIQIAASEVGHV
                     PTLVFDEVDTGIGGAVAATVGRMLQTLGARRQVLCVTHLPQVAAHADHHFQVAKSGDD
                     EQATSTLHALGRVNRVDELARMLGGSEITPKTRAHAAELFAQHRRRES"
     gene            complement(8090..9037)
                     /gene="pbpG"
                     /locus_tag="VFO53_00325"
     CDS             complement(8090..9037)
                     /gene="pbpG"
                     /locus_tag="VFO53_00325"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HET8874746.1"
                     /translation="MLMKRLLPIVFVTLVAGSAWADTGMPDPTRLRLASANVLVLDAA
                     ADRQIYAKAADEVTPIASVTKLMTAMVVLDSQQALDDVLEIDTADYDLLKNSSSRLRA
                     GTTLSRREMLRLALMSSENRAASSLARHFPGGKQAFVTAMNFKAASLGLTHTHYSDPT
                     GLSPENVSTASDLAKLVQAAAEYPMIREFTTTPSYLVEMDTGRTLGFNNSNALVKKQD
                     WDITLQKTGYIREAGKCVVMLVNIASKPFVIVLLDSLGKYTRIADAQRVKHWLETGES
                     LALAPVRAARAVKVRAASHLHRRTHGTLHPRLVKTSQKH"
     gene            complement(9111..11027)
                     /locus_tag="VFO53_00330"
     CDS             complement(9111..11027)
                     /locus_tag="VFO53_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HET8874747.1"
                     /translation="MRHASSMPLPADTHVPRTERGDWQTIRTLLPYLWGYRGRIGAAL
                     TCLVLAKVANVGVPVLLKRIVDSLDPRMAALAVPLALLVAYGLLRLSTTVFTELREFL
                     FAKVTQRAVRRIALQVFRHLHALSLRFHLARQTGGLTRDVERGQRGISTLISFALFSI
                     LPTLVEISFVSAILIVRYDWTFIAITVVALALYILFTVMITEWRTHFRRQMNDLDSRA
                     NTRAIDSLINYETVKYFGNEEWEASRYDESLRHWERAAVLSQTSLSTLNIGQSAIIAV
                     AVTLIMWRATAGVVDGAMTIGDLVLVNAFMIQLYIPLNFLGVIYREIKQALADMERLF
                     RLIEEHADIADREGARPLVVHGAEVRFAHVDFAYEPNRSILHDVTFTIPAGRNVAVVG
                     PSGSGKSTLARLLYRFYDASGGRITIDGQDIRDVQQQSLRAAIAIVPQDTVLFNDTIE
                     YNIAYGRPGATHDEIVQAARLAQIHDFVQRLPEGYATPVGERGLKLSGGEKQRVAIAR
                     AILKRPAILIFDEATSALDSRSEQAIQAELRLIARDHTSLTIAHRLSTIVEADEILVL
                     EHGRIIERGTHVALLAANGAYARMWRLQQHERRDDGTPAEAGDAGLPSEPADVHEHNP
                     APAAAAAATLRSAS"
     gene            complement(11105..12292)
                     /gene="clsB"
                     /locus_tag="VFO53_00335"
     CDS             complement(11105..12292)
                     /gene="clsB"
                     /locus_tag="VFO53_00335"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HET8874748.1"
                     /translation="MNRYTAGNSVTLLRNGAEYFPVLLEAIALAEREIWLETYIFADD
                     DTGRSVADALIGASRRGVDVRVLVDGWGARHYLTHALAQRLIDGGVDLLKYRPEVMPW
                     HFRSHRLRRLHRKLCHADGRVAFVGGINVIDDMNTPGHTPPRVDFAVCVRGPLVVPIA
                     QTMQRVWAIVEVTQLRHSDVPFFPDEEPAENAGTQVAKFTIRDNLRHRRDIERAYLAA
                     LRTSRHEIVIATAYFFPGVRFRRALLSAAARGVKVTLLLQARVEFRLLHHASRALYGQ
                     LLAAGVAIQEYHRSFLHAKVAVVDAQWATVGSSNIDPYSFLMAREANVFVRDPVFASG
                     LRAELAKMIEGGSRPVPPQDWAQRSRLAKAASWIAYGTVRVAMGMMRYGGDEWWRGNP
                     PAQ"
     gene            complement(12314..13114)
                     /locus_tag="VFO53_00340"
     CDS             complement(12314..13114)
                     /locus_tag="VFO53_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET8874749.1"
                     /translation="MSSTPVRFTVATYNIHKGFSQLARRMVIHELRDRLRGLAADILF
                     LQEVLHTNDRHATRYREWPTRPQHEFIADAFWHEVAYGRNCVYPHGHHGNAVLSRFPM
                     LAQENLDISAHLFESRGLLHCEVALGDALPSLHCINVHLGLFERGRQWQIQALIDRIQ
                     ISVPREAPLVIAGDFNDWRSKGDRALTEALDLVEVFGEVRGRPARTFPSMLPVFRLDR
                     IYARGLRVVDAHVHYATRGARMSDHAALAATFEVTSAERDSRLAVNGD"
     gene            complement(13243..13944)
                     /locus_tag="VFO53_00345"
     CDS             complement(13243..13944)
                     /locus_tag="VFO53_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET8874750.1"
                     /translation="MLKFRKSADRGYADHGWLKSYHSFSFAGYHDPANMGWGNLRVIN
                     EDRVAPGMGFGRHSHRDMEIISYVLSGELAHQDNLGNVKGIPPGDVQRMSAGTGVTHS
                     EFNHAEGETTHFLQIWIEPNVIGIPPSYEQKTIPAEQKRGTLRLVASPDGARGSVTIH
                     ADAAIYAGLFDGSETAELALRPTRKGYVHLIRGSIDVNGQPIESGDAALLEGESRIVL
                     AHGADAEVLVFDLAA"
     gene            complement(13981..14056)
                     /locus_tag="VFO53_00350"
     tRNA            complement(13981..14056)
                     /locus_tag="VFO53_00350"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14021..14023),aa:His,seq:gtg)
     gene            complement(14158..15474)
                     /locus_tag="VFO53_00355"
     CDS             complement(14158..15474)
                     /locus_tag="VFO53_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET8874751.1"
                     /translation="MSPTHAGILVLVVTLAMLLSGAPVAFGLGAISILFVLIFQGFDA
                     LHVVAETFYSGLNDFTLVSIPMFVMMGAAIGSSPAGKDLYEALDRWLYRVPGGLVISN
                     LGACALFAALTGSSPATCAAIGKMGIPEMRRRGYPDDVATGSICAGGTLGILIPPSIT
                     FILYGIATETSIGRLFLAGVFPGLLLTALFMLWTLFIIWKRGFRAHAAEFRYTWAQKF
                     GSIPKVAPFLAIVVGVMYVLYGGVATPSEAAGVGAALCVVLAVIIYRMWRPSQWWQIL
                     RDTTRESVMILMIIATAVLFGYMLTSLYLTQTLAHAIADMQVNRWVLMGMINVFLLVC
                     GFFIPPAAIILMTSPILYPIVRAAGFDPVWFGVIVTINMEIGLIHPPVGLNIYIVNAI
                     APDVPVTRVMWGTLPYVACMMLAIVILCAFPEIATWLPDRLMGAHQ"
BASE COUNT         2606 a         5244 c         5297 g         2476 t
ORIGIN      
        1 gagcgactcg agcttcgcgc gcgtcagctt catcgacagg tgcttcggtc ccgactggtc
       61 ggccgtgatg tacggcaggt tgatgtcggt ctgctgcgaa ctcgacagct cgaccttcgc
      121 cttctcggcc gcttccttca accgctgcag cgccagcacg tcgttcttga gatcgacgcc
      181 cgcttccttg cgaaactcgt tgacgacgta ttcgatgatc cgctggtcga agtcctcgcc
      241 accgaggaac gtgtcgccgt tggtcgacag cacctcgaac tggtgctcgc cctccacgtt
      301 cgcgatctcg atgatcgaga tgtcgaacgt gccgccgccg aggtcgtaca cggcgatctt
      361 gcggtcgcct tccttcttgt cgagcccgaa cgcgagagct gccgccgtcg gctcgttgat
      421 gatgcgcttc acctcgagac ccgcgatgcg gccggcgtcc ttggtcgcct ggcgctgcga
      481 gtcgttgaag tacgcgggca cggtgatcac cgcgtcggtg acttcctcgc cgaggtagtc
      541 ctcggccgtc tttttcatct tgcgcagtat ttccgcggac acctgttgcg gcgcaatctt
      601 cttgccgcgc acctcgaccc aggcgtcgtt gttgtcggcc ttgacgatcc tgtagggcat
      661 caggttgatg tccttctgca cttccttttc ctcgaagcgc cggccgatga gccgcttcac
      721 cgcgaagatg gtgtttttcg cgttggtgac cgcctgccgc ttcgcgggcg cgccgacgag
      781 aatttcaccg tcttccgcgt acgcgacgac cgacggcgtc gtgcgtccgc cttccgagtt
      841 ctcgatcacc ttgggcgctc cgccctccat gatcgcgacg cagctgttcg tcgtcccgag
      901 gtcgatgccg atgatctttg ccattttcga attgcctccg tgctccggaa tgtgttgatc
      961 tgaacgtgga aatggggttt ctgaagggcg aatcaacccg cgcgcagcta cgatccctgc
     1021 tgcgccttcg acaccgtcac cagcgccggg cgcaggacgc gatcgttcag caggtaaccc
     1081 ttctgaaaaa cggtcacgac cgtattgggc ggaaggtcgc tgtcgacgac ctgcatcgcc
     1141 tgatgcacgt gcggatcgaa cttctcgccg gccgggttga tttcgcggat ggccgcgcga
     1201 ccgaacgccg cgttgagcgc cttcagcgtg aggtcggaac cggccttgag cgtttcggcc
     1261 gcgaccgact ggtcggtcgc cagcgtctgc tccagcgcat ccttcacggc gagcaggtcc
     1321 ccggcgaatt tctcgagcgc gtacttgtgc gcccgggcga cgtcggcctg accctggcga
     1381 cggacgttct cgacctcggc gcgcgcccga agccacgcat ccttcatgtc ggcggcttcg
     1441 gcgcgggcct tcgcaagcgc gtcctcgccg gacaccgcgt cctgcggacc ggcagcgtcg
     1501 gaggcgttat cgtcgatatg atcgaatccg gtacggcgtt cttggtctgt catcgtggca
     1561 atgcgcggtg ggaagtagaa acggacggac gccccttcag attgggcgcc ggggcggccc
     1621 tttcaactgg agaacgggag cccgacagga gaacgggagc ccggcaggag aacggcagcc
     1681 gcgaaccgct agccggcggc gcccagtgcc ttcgcgcgga gccgcgtcgc ttcgcgctcg
     1741 gcgatgccgg gtggcggcgc gagccatccg tgcaggttgg cgagcacgat ctcggtcaac
     1801 gccgcaatcc agcgcgggtg ctcgttcagg cacgcaatga cgtcgtaatc gccaccgcct
     1861 gcggcgcggt agacgcgctt gccctcgatc gccagctctt ccagcgtttc gaggcaatcg
     1921 gcgacgaagc ccggggcgaa gacgtcgacg cgccgaagcc gcgccttgcc gagcttggcg
     1981 agcgtcgccg ccgtatacgg ctcgagccag cggccgcgcc cgaagcgcga ctggaacgcg
     2041 agcgtccact gcgacgtctc gagcccgagc tcgcgcgcca gcaggcggcc cgtggcctgg
     2101 cagtagcagt gatacggatc gccgcgggcg agtgcgcgcc gcggcaatcc gtggaaggac
     2161 agcacgaggt gctcgggttg cccgtgcttc atccagtgat cgttgacggt cgccgccagc
     2221 gccgcgatgt agccgggatc gtcgtggaac gtgtcgacga agcgcagccc cggcacgcgt
     2281 cgcgtatggg cgagatgcgt ggcgaccgca tcgacgacgg acccggtcgt gctcgacgcg
     2341 tattgcggat acatcgggac cacgaggatg cgctcgcagc ccgcgccgcg cagccgctcg
     2401 aggccggcgc cgatcggcgg cgcgccgtag cgcatcgcct gctcgaccac gacggcgtcg
     2461 gccgcgagcc cgagcgcctt cagccgctgg ccgagcgagc ccgacagcag caccttctgc
     2521 ttgttcgcat gaacgccgag cggcgatccg tccttcgtcc agatcgccgc gtagcgcgcg
     2581 gccgatcgcg ccggtcgcgt gcgcagcacg aagccatgca gcagcggccg ccaggcaagc
     2641 ggcgggatct cgacgacgcg cggatcggcg aggaactcgg cgagatagcg ccgcaccgcc
     2701 gatgcggtgg gcgcgtccgg tgtcccgagg ttgacgagca gcacgccgac cttcggcgtg
     2761 cggtcgtgcg cgaacggcgg ctcgggtcgg aacgccatcg cgctacgcgt cccgcaggcg
     2821 ctcgacaagc cgatcgatcg cgccaacgcg cgcgaacgcg gcgggcgtca tcggaatgcg
     2881 cgcgcgcacg tcgtccgacg acgcgaacgt gcgaaaccgg cgccgatagc gggcgcgaag
     2941 ctcgcgatcg ttcgcgagct cgaggaagcg gagctgcagg tagtgctcga gcaggtcctt
     3001 cgtttccggt gcacctgcgc tcgtgccgcc caggtgcacg accgcgccgc cggccgagcc
     3061 gcgaaaaccg agcgtgccgc cctcggccag cgcgagcgcg acgagcaggt gcagcgtgtc
     3121 gaggtaatcg tgatgatcct tgaaggcgac gccgtcgcgc agtccgatcc tcgcgacgag
     3181 cgcgcgaagg cgcgccggaa cccgccgata gacccgcgcg tcgacgcgat gacggcgcat
     3241 gatatcgcgt aaaagcggtg cattgaggat caggaagaac gtctcggcgt acgcgagtcc
     3301 cgacttcctg ccgaccgaac ggaaccaccc ggtcaggcac tccgccggcg caggcgtggc
     3361 accttcgaag caggtcgcgt cgaatacgta gcaatcgtga tcgagcaggc cgaacggcgc
     3421 agcgttgcgt tcgagcagca gcgtgatgac gtcaccgtgc gcgaggctcg accccggcag
     3481 cgcggcgagc gtgacgatgg gtcgcgccgg aaacgcagca accagccggc gccgctccca
     3541 cgcccgcgcg ccgttcgcaa tcaggtgcac cgtctgctgc gatggcagaa ggcgcaggca
     3601 cggcagcagg aagtgcagcg tcccgggcat cacgatcacg tagaaatggc cgccgctgct
     3661 tgcgttcgtg cgatgaaagt gatcgaggcg ccggcggttg atcgtccgca gcggccatgc
     3721 gccgttgatc cttcgcgcca acgcacgata cgcgcgctcg gccgtcaagg ccggaagggc
     3781 gaaagcgatc gccacgaacg gcgagcggcc gccggcggtc aagcgtgcga ccgcctgctt
     3841 cagctgccgc agattggcag tgatccgaga cgtcgtatgt cagttgcgaa gggccgcccc
     3901 gagcaactga tccacccccg ggggggcagc gagcgcagcg agcgtggggg gacgtgtcat
     3961 cctagtcgtc gagcagttcg cgcgacagcg cgttcgaaag cagccgggcg gtcatgtcga
     4021 cgatcgggat cacgcgctcg taggccatgc gcgtcggacc gatgacgccg agcgtgccga
     4081 tcacctgccc gttcgcctcg tagcttgcgg tcacgacgct gcactcgtcg agcggcacga
     4141 ggcccgattc gccgccgatg tagatctgca cgcccggcgc gcgctgcgag atgtcgagca
     4201 ggtgcagaag cgatgtcttc tgctcgaaaa gatcgaagag ccggcgcagc cgctgcatgt
     4261 tcgacgcgag gtcctcggcg ttcagcagat tgcgctcgcc ggtgacgacc agcgcgtcgg
     4321 cttgcgacag cgcaccctcg cccgcgtcga cggcggcctt catcagcgag gcaatgtcgt
     4381 cacgcagtgc ccgcaactcg tcggccagcc ggtcgcggat gccggtgaac ggatggcccg
     4441 cgaagttctg gttgaagaag ttggtcgcct cgacgagctg cgacggcgtg tacgagcgat
     4501 cggtgtgcag gatgcggttc tgcacgtcgc cttccgccgt gacgatgatg agcagcacgc
     4561 gccgctcgga aaggcgcagg aactcgaggt gacgaaacga cgcctcgcgc cgcttgggcg
     4621 ttgcgacgac gcccgcgaac tgcgtaagct gcgacaaaag cgatgccgcc gcgttcacga
     4681 tctgttgcgg gcgatcgacg ttgagctcgc cttcgagacg gtgaatctcg acgtcctcga
     4741 gcggctgcac gaccatcagg ctgtcgacga agaagcggta cccgcgaggc gtgggcacgc
     4801 gccccgcgga cgtatgcggg ctcgcgatca ggccgatttc ctcgaggtcg gccatcacgt
     4861 tgcgaatcgt cgcgggcgac agatcgagcc ccgaatggcg cgacagcgcg cgcgagccga
     4921 cgggttgccc ttcggcaacg taccgctcga cgaggatttt cagcaggtgc tgggcgcgcg
     4981 cgtcgagcat gtcgagcccg gcgggacgcg caccatccga tcgcatcatt tcgccaatct
     5041 taccctgtcc tccggcgggg cagcgcgtcg gccaagcggc cgacacgagg cggacgccgc
     5101 gctatggttt aatccgcgca tgtccgcccg cacgccggaa cgctttcacc ggatcgcact
     5161 catcgggcgt catggcggcc ccgccgcggc cacgccggtc gccacgctcg ccgcgttgct
     5221 cgtcgcgcgc gggcacacgg tggtcctcga acccgacgcg gcgggcggcc tggttcttcc
     5281 gggcgtcacg acgctgtccg acgacgcgct gcccgacggc gtcgatctcg cgatcgtgct
     5341 cggcggcgac ggcacgatgc tgtcggtcgc gcggcgcctc gcaccccacg acgtgccgct
     5401 gatcggcgtc aacctcggac gcctcggctt cctcaccgac attccgctcg cgcgcatgga
     5461 gcccacgatc gcggcgatgc tggacggccg ttacaccgaa gagcggcgaa cgctgctctc
     5521 ggtcacgctt tgtcgcgccg acggcaccag cacgtcggca ccggcattga acgaggtcgt
     5581 cgtgaaccgc gggggtctcg gcagcatgat cgagtgcgcc gtcgaggtcg acggccgctt
     5641 cgtctacgcc atgcgggccg acggcgtgat cgtcgccacg ccgaccggct ccaccgcgta
     5701 tgcgctgtcg accggcgggc ccatcatcgc gcccgaagtg ccggcgttcg cactcgtccc
     5761 gattgcgccg cacgcgctga cgcaccgccc gatcgcgctg cccgaccggt cgaccatcac
     5821 catcacggtg agccacggcc gcgacgccgc cgtgcattgc gacggacagg cgcacttcga
     5881 gctcgccgag ggcgaccgcg tcaccgcgcg gcgcgcgccg cacaccgcgc gcctgctgca
     5941 ccccgaatac cacgactatt tcgcgatgct gcgcgcgaag ctgcactgga gcgaaacacc
     6001 cgagcggcta acgcagggcg atgcgcggta gggcctgcta acgctatggg cgcgaacgcg
     6061 ttgcaccgcc gaaaacgccc agcgcaaggc gcgaaggcga aggcgggctc gttgcctggc
     6121 gagacttcgc aacgcagcga tgggcgcttt cggccgcaac ccggagggaa caggtcgcct
     6181 ggggcgccac tctgcgttgt tctcctcgcg aagacgcccg gtcttcgctt cgtcggccgc
     6241 cttgattgtc gccccaggcg acctgttcgc gttcgtgtcc atagcgttag caggccctac
     6301 gtgcgtgcgt tcccgtccgg atcgggctga ttccttccgt cccgttcgtc cgcgacgcaa
     6361 tgctcagcgc actgtcgatt cgtcatttcg tcgtcgtcga cacgctcgac gtcgaattcg
     6421 ctcccggctt cacggtcctg actggcgaaa ccggcgcggg caagtcgatc ctgctcgacg
     6481 cactgggcct gctgctcggc gaccggttcg acacgcgcca ggtgcgaagc ggtgccgagc
     6541 gcgccgagct tgccgccgtg ttcgccatcg gcggatcgtc cagcgtcaag aattggctca
     6601 acgcggaggg cctcgacgcc ggcaccgagc tgctgctccg ccgcacgctc gatgtgcagg
     6661 gacgaagccg cgcctggatc aacggccgcc cggcgacgct cgcacagttg accgagctcg
     6721 gtgcgcaact gctcgacctg catgggcagc acgcacacca ggcgctgatc ggtgccgaag
     6781 cgcagcggac gctcctcgac gaattcggcg gcttcgcatc gtcggcggcg gacgtcgccg
     6841 acgcgtggcg cgcgtggcgg gttgccgtcg agcgcagcga agcggcggcg gatgccgacg
     6901 ccgcattcgc ggccgagcgc gacgcgctgc gcgagcgcga aagcgagctt gcggagctcg
     6961 cgctcgcgcc cgacgaatgg aacgcgctca tcgcgaccga gcggcgtctc gtcaacgcgg
     7021 gcgcactgat cgaggtcgcg acccaggccg aggacgcatt gagcgaaggc gacgacgcga
     7081 tcggccggcg cctcgcgcgc attgcgcaga aacttgcgca ggcgagcgcc gacgacgaag
     7141 cactcgcgcc gatcgtcgcg ctgctcgagc ccgcggcgat ccagctcgac gaggccgcac
     7201 gcgcggtgcg cgaataccgg cgccggctcg acctcgatcc ggacgagctc acgcgcgtgc
     7261 aggagcgtct ctcggccatt cacgacgtcg cgcgcaagca ccgcgtgcga cccgacgcgc
     7321 tgcccgagtt ggcacttgcc acgcgtgcgc ggctggtgga gctcgcgcag gcgaccgatg
     7381 cgacggcgct cgcggaggcg gcgagcgaag cgcgacgccg ctacgacgcg ctcgcgcgga
     7441 cccttacgca gcaacgggcg ggcgccgccg cgacccttgc gcagcgcgtg accgcggcaa
     7501 tgcaggagct cgcgatggcg ggcggccgct tcgacgtggc actcgaggcg ctgccgctgc
     7561 cggccagctc cggccgcgag agcgtcgcgt tccgcatcgc gacgcatccg aagcagccgc
     7621 cactgccgct gtcgcgcgtt gcgtcgggcg gcgagctttc acggctggcg cttgcaatac
     7681 agatcgccgc gagcgaagtg ggccacgtgc cgacgctcgt tttcgacgaa gtcgacaccg
     7741 gcatcggggg cgccgtcgcc gccaccgtcg gacgcatgct gcagacgctc ggcgcgcgcc
     7801 ggcaggtgct ctgcgtaacg catctgccgc aggtcgcggc gcacgccgat catcatttcc
     7861 aggttgcgaa gtcgggcgac gacgagcagg ccacgagcac gcttcacgcc ctcggaaggg
     7921 taaatcgagt cgacgagctg gcgcggatgc tgggcggaag cgagatcacg ccgaagacgc
     7981 gtgcgcatgc ggcggagctc ttcgcgcagc accggcgcag ggaaagctga ccaaaaaccg
     8041 atgcagggtc agagacgact tttcgctgcg cgaaaactcg tctctgaccc taatgcttct
     8101 gcgacgtctt gacgagccgc gggtgcagcg ttccgtgcgt gcgacgatgc aggtgcgatg
     8161 ccgcgcgcac cttgaccgcc cgcgcggccc gcacgggcgc cagcgcgagc gactcgccgg
     8221 tttcgagcca gtgcttgacg cgctgcgcat cggcgatgcg cgtgtacttg ccgagcgagt
     8281 cgagcagcac gatcacgaac ggcttgctcg cgatgttgac gagcatcacg acgcacttgc
     8341 ccgcttcacg gatgtagccg gtcttctgca gcgtgatgtc ccaatcctgc ttcttcacga
     8401 gcgcgttgga attgttgaag ccgagcgtcc tgccggtgtc catctcgacc agatacgacg
     8461 gcgtcgtcgt gaactcgcgg atcatcggat actcggctgc ggcttgcacg agcttcgcga
     8521 ggtcgctcgc cgtcgacacg ttttcgggag agagaccggt gggatcggag taatgcgtgt
     8581 gcgtgaggcc gagcgaggcg gccttgaagt tcatcgccgt gacgaacgcc tgcttgccgc
     8641 ccgggaagtg gcgcgcgagc gacgacgcgg cacggttctc cgacgacatc agcgcgagcc
     8701 gcagcatttc gcggcgcgac agcgtggtcc ccgcgcgtag ccgcgacgag ctgttcttca
     8761 gaagatcgta atcggcggtg tcgatctcga gcacgtcgtc gagggcctgc tgcgaatcga
     8821 gcacgaccat cgccgtcatc agcttggtga ccgacgcgat cggcgtcact tcgtctgccg
     8881 cttttgcgta aatctgacga tccgcggcag catcgaggac gaggacgttg gccgaggcga
     8941 gcctcagccg ggtgggatcg ggcatgcccg tgtctgccca ggcggacccg gcaacaagag
     9001 tcacgaaaac aatcggtagc agacgtttca tcagcataat tctcaagtgt gccgacgggt
     9061 ttatcgcacg ctcgcgaccc cgccgtcaac aggtaatcgc caatcggctg ctaggaagcg
     9121 ctgcgcagcg tcgcggcggc cgcggccgcc ggtgccgggt tgtgctcgtg cacgtcggcg
     9181 ggctcgctcg ggagtccggc gtcgccggct tccgcgggcg tgccatcgtc ccgccgctcg
     9241 tgctgctgca accgccacat ccgcgcgtac gcgccgttgg cggcaagcag agcaacatgt
     9301 gtgccgcgct cgatgattcg cccgtgctcg agcacgagga tctcgtcggc ttcgacgatc
     9361 gtcgacaacc ggtgcgcgat ggtgagcgac gtatggtcgc gcgcgatgag ccgcagctcg
     9421 gcctggattg cttgctccga gcgcgagtcg agcgcggacg tcgcttcgtc gaagatcagg
     9481 atggccgggc gcttgagaat cgcgcgcgcg atcgcaacgc gctgcttctc gccgcccgac
     9541 agcttgaggc cgcgctcgcc gaccggcgtc gcatagcctt ccggcaggcg ttgcacgaag
     9601 tcgtggatct gcgcgagcct cgccgcctgc acgatttcgt cgtgcgtcgc cccgggacgt
     9661 ccgtacgcga tgttgtattc gatggtgtcg ttgaagagca ccgtatcctg cgggacgatg
     9721 gcgatcgccg cgcgcagcga ctgctgctgc acgtcgcgga tgtcctggcc gtcgatcgtg
     9781 atgcggccgc cgctcgcgtc gtagaagcgg tacagaagcc gcgcgagcgt cgatttgccc
     9841 gagcccgacg gccccacgac agccacattg cggcccgcgg gaatcgtgaa cgtgacgtcg
     9901 tgcaggatcg accggttcgg ctcgtacgcg aaatcgacat gcgcgaaacg cacttccgcg
     9961 ccgtgcacga cgagcgggcg cgcgccttcg cggtcggcga tatcggcgtg ctcctcgatc
    10021 aggcggaaca gccgctccat gtcggcgagg gcctgcttga tctcgcgata gatgacgccg
    10081 aggaagttga gcggaatgta gagctggatc atgaacgcgt tgacgagcac gaggtcgccg
    10141 atcgtcatcg cgccgtcgac gacaccggcc gtcgcccgcc acatgatcag cgtcacggcg
    10201 accgcgatga tcgcgctttg cccgatattg agcgtcgaca gcgacgtctg cgacagcacg
    10261 gccgcgcgtt cccagtggcg aaggctctcg tcgtaacgcg acgcctccca ttcctcgttg
    10321 ccgaagtact tgaccgtctc gtagttgatg aggctgtcga tcgcacgcgt gttcgcacgc
    10381 gagtcgaggt cgttcatctg gcggcgaaag tgcgtccgcc actcggtgat catcacggtg
    10441 aacaggatgt agagcgcgag tgcgacgacc gtgatcgcga tgaacgtcca gtcgtagcgc
    10501 acgatgagga tcgccgacac gaacgagatc tcgacgagcg tcggcaggat cgagaacagc
    10561 gcgaagctga tcagcgtcga gatgccgcgc tgtccgcgct cgacgtcgcg ggtgaggccg
    10621 cccgtctggc gtgcgaggtg gaaacggagc gaaagcgcat gcagatgccg gaacacctgc
    10681 aaggcaatgc ggcgcacggc gcgctgggtc accttggcga acaggaattc ccggagctcg
    10741 gtgaacaccg tcgtcgacag ccgcagcagg ccgtacgcaa cgagcagcgc gagcggcacg
    10801 gcgagcgccg ccatcctggg atcgagactg tcgacgatcc ttttcagcag caccggcacg
    10861 ccgacgttcg cgaccttcgc cagcacgagg caggtcagcg ccgcgccgat gcgcccccgg
    10921 tagccccaca ggtacggcag cagcgtgcgg atcgtctgcc agtcgccgcg ctcggtgcgc
    10981 ggaacatgcg tgtcggcggg cagcggcatc gacgaagcgt ggcgcatcgt ttcagtctag
    11041 cagccggcgc cgcgcatcaa cgcgctcggt ccgggcgcgt tgcagaaccg cgtgccgcga
    11101 gcggtcattg cgccggcgga ttgccgcgcc accactcgtc gccgccgtag cgcatcatgc
    11161 ccatcgcgac gcgcacggtt ccatacgcga tccagctcgc ggccttcgcg aggcgcgagc
    11221 gctgcgccca gtcctgcggc ggaacgggtc gtgaaccccc ttcgatcatc ttcgcgagct
    11281 cggcgcgcag cccgctcgcg aagacggggt cgcgcacgaa cacgttcgct tcgcgcgcca
    11341 tcaggaacga gtaggggtcg atgttcgacg agccgacggt cgcccattgc gcgtcgacga
    11401 ccgccacctt cgcatgcagg aacgagcggt gatactcctg gattgcgacg ccggcggcga
    11461 gaagctgtcc gtaaagcgcg cgcgacgcat ggtgcagcaa gcggaactcg acccgcgcct
    11521 gcagaagcag cgtcaccttg acgccacgcg cagccgcgct cagcagcgcc cggcgaaagc
    11581 gcacgcccgg aaagaagtac gcggttgcga tgacgatctc gtgccgcgaa gtgcgcaacg
    11641 ccgcaaggta cgcgcgctcg atgtcgcgcc ggtggcgcag gttgtcgcga atcgtgaact
    11701 tcgctacctg cgtgccggcg ttctccgcgg gctcctcgtc ggggaagaac ggcacgtccg
    11761 agtggcgcaa ctgcgtcacc tcgacgatcg cccacacgcg ctgcatcgtc tgcgcgatcg
    11821 gcacgaccag cggcccgcgc acgcacacgg cgaaatccac gcgcggcggc gtatggccgg
    11881 gcgtattcat gtcgtcgatc acgttgatgc cgccgacgaa cgcgacgcgg ccatccgcat
    11941 ggcaaagctt gcgatgcagc cggcgcagcc ggtgcgagcg aaagtgccat ggcatcactt
    12001 cggggcgata tttcagcaga tcgacgccgc cgtcgatcag ccgttgcgca agtgcgtgcg
    12061 tcagataatg ccgcgcgccc cagccgtcga cgagcacgcg aacgtccacg ccccgccgcg
    12121 acgcgccgat gagcgcatcg gcgacgcttc gcccggtatc gtcgtcggcg aagatgtagg
    12181 tctcgagcca tatttcacgc tcggcaagcg cgatcgcctc gagcagcacg gggaagtatt
    12241 cggcgccgtt gcgcagcagc gtcaccgaat tccccgccgt gtagcggttc atcaccgggc
    12301 gtctggcatc gggtcagtcg ccgttcaccg cgagccggct gtcgcgctcc gccgaggtca
    12361 cctcgaatgt cgcggcgagc gccgcgtggt cggacatccg cgcgccgcgc gtcgcgtagt
    12421 ggacgtgcgc gtcgaccacc cgcaagccgc gcgcgtagat gcgatcgagg cgaaacaccg
    12481 ggagcatcga cggaaacgtg cgcgccggcc ggccgcgtac ttcgccgaag acctcgacga
    12541 gatcgagcgc ctcggtgagc gcccgatcgc ccttgctgcg ccagtcgttg aaatcgccgg
    12601 cgatgacgag cggcgcctcg cgcggcaccg agatctggat gcggtcgatc agtgcctgga
    12661 tctgccattg ccggccgcgc tcgaacaggc cgaggtgcac gttgatgcaa tgaaggctcg
    12721 gcagcgcgtc gccgagtgcc acctcgcagt gcagaagacc gcggctttcg aaaagatgcg
    12781 ccgagatgtc gagattctcc tgcgcgagca tcgggaagcg cgacagcacc gcgttgccgt
    12841 ggtggccatg ggggtacacg cagttgcggc cgtacgccac ctcgtgccag aacgcgtcgg
    12901 caatgaattc gtgttgcggc cgcgtgggcc actcgcgata acgcgtcgca tgccggtcgt
    12961 tcgtgtgcag cacttcctgc aggaacagga tgtcggccgc aagcccgcgc agccggtcgc
    13021 gcaactcgtg gatgaccatc cgccgcgcga gttgcgagaa gcccttgtgg atgttgtagg
    13081 ttgcgacggt gaagcggacg ggcgtggatg acatgcgtgc aatgttagcg cgctacctcg
    13141 ccgcacgcgt tgttccgctc ttgcccacgt gagcaattag cgcacgccgc gggtcgagtc
    13201 gcgacgacgc ctgtgcctgc ggcacgatgc cgaagcagaa aatcaggccg ccaggtcgaa
    13261 cacgagcacc tcggcatcgg ccccgtgggc cagcacgatc cggctttcgc cttcgagcaa
    13321 tgcggcatcg cccgactcga tcggctggcc gttgacgtcg atcgagccac ggatcagatg
    13381 cacgtagcct ttgcgtgtcg gccgcagcgc aagctccgcg gtctcgcttc cgtcgaacag
    13441 gccggcatag atggcggcgt cggcatggat cgtcaccgag ccccgagcgc cgtcgggcga
    13501 ggccaccaaa cgcagggtgc cgcgcttctg ttctgcagga atcgtcttct gctcgtaact
    13561 cggcgggata ccgatcacat tcggctcgat ccagatttgc agaaagtgcg tggtctcgcc
    13621 ttcggcatgg ttgaactcgc tgtgcgtcac gccggttccc gcgctcattc gctgcacgtc
    13681 gcccggcggg atgcccttga cgttgccgag gttgtcctga tgggcgagtt cgcccgacag
    13741 cacgtagctg atgatttcca tgtcgcggtg actgtgcctg ccgaagccca tgccgggcgc
    13801 cacgcggtct tcattgatca cgcgcaggtt gccccacccc atgttggcgg ggtcgtgata
    13861 gccggcaaac gagaacgagt ggtagctctt gagccagccg tgatcggcgt agccgcggtc
    13921 cgcggatttg cgaaacttga gcatcgcgag ccttcgccga cgcgatccga tgcgtgcgag
    13981 tggggtggct gatgggactc gaacccacga caaccggaat cacaatccgg gactctacca
    14041 actgagctac agccaccgcc ggagaacgag gcgtcgttgc agacgcatcc agcgccgatt
    14101 atacccctgg ccgatcgatg cccgcactgc gcgggagacc gcttcaccgc cgcttcgtca
    14161 ctggtgcgcg cccatcagcc ggtcgggcag ccaggtcgcg atctccggaa atgcgcagag
    14221 gatgacgatc gccagcatca tgcaggcgac atagggaagc gtgccccaca tcacgcgggt
    14281 caccggaacg tcgggagcga tcgcgttgac gatgtagatg ttgagaccca ccggcgggtg
    14341 gatgaggccg atctccatgt tgatcgtgac gatcacgccg aaccacacgg ggtcgaaccc
    14401 ggcggcgcgc acgatcgggt agaggatcgg gctcgtcatc aggatgatcg cggccggcgg
    14461 gatgaagaag ccgcagacga gcaggaatac gttgatcatg cccatcagca cccagcggtt
    14521 gacctgcatg tcggcgatcg catgcgcgag cgtctgcgtc aggtacagcg acgtcagcat
    14581 gtagccgaac agcaccgcgg tggcgatgat catcaggatc atcaccgact cgcgcgtcgt
    14641 gtcgcgcagg atctgccacc actgcgacgg gcgccacatc cgatagatga tgaccgcgag
    14701 caccacgcaa agcgcagcgc cgacgcccgc cgcttccgac ggtgttgcga ccccgccgta
    14761 gagcacgtac atgacgccga cgacgatcgc gaggaacggc gcgaccttcg gaatcgagcc
    14821 gaatttctgc gcccacgtgt agcggaactc cgccgcatgc gcacgaaatc cgcgcttcca
    14881 gatgatgaac agcgtccaca gcatgaacag cgcggtcagc agcaggccgg gaaagacgcc
    14941 ggcgagaaag agccgcccga tcgacgtttc cgtcgcgatg ccgtagagga tgaacgtgat
    15001 cgacggcgga atcaggatgc cgagcgtgcc gccggcgcag atcgagcccg ttgccacgtc
    15061 gtccggatag ccgcggcggc gcatttccgg aatgcccatc ttcccgatcg ccgcacacgt
    15121 cgccggcgac gagccggtca gtgccgcgaa gagcgcgcag gcgccaagat tggagatcac
    15181 gaggccgccc ggaacgcgat agagccagcg gtcgagcgcc tcgtagagat ccttgcccgc
    15241 cggcgacgac ccgatcgccg cgcccatcat cacgaacatc gggatcgaca cgagcgtgaa
    15301 atcgttgagt cccgaataga acgtctcggc gacgacgtgc agcgcgtcga agccctggaa
    15361 gatcagcacg aacaggatcg agatcgcgcc aagaccgaac gccaccggcg cgcccgacag
    15421 cagcattgcg agtgtcacca cgaggacgag aatgccggcg tgcgtcggac tcaccgggtg
    15481 gccttccgtg atctcattcg ctgagcgccg cgcgagcggc gcgtgcggcc ctggcggcgg
    15541 cgtcgtcggc gtcgtcgatg ccgaacggcg cttcacgccc ggtgacgagg catgcaatgt
    15601 cgacgacgta ctggagcgtc agc
//
LOCUS       DASSVX010000006         4287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1000000, whole genome shotgun sequence.
ACCESSION   DASSVX010000006 DASSVX010000000
VERSION     DASSVX010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000000"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VFO53_00360"
     CDS             complement(<1..1380)
                     /locus_tag="VFO53_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874752.1"
                     /translation="MRSLRAVLRRALSMVWVGLVASTCVAQPLRAADALLAIDEQRAS
                     VVERIVAQWGGPLAKSSAPIGVDDLRDRLMGLRADQLLAASLTGTLQGMRAVLGVDEL
                     AKPAPGQPKLAPAGSGAVYTPVTPCRLVETRGTFAAVYQGDGSASHHALPFAPSEIRS
                     YKLQGGNGVCLTQLPAGLDPSAVQLQVFGIPTTSASGDIEILPQGASFGSTATMVYVG
                     SIAFNTVSTSAKVNLADNEISVQVRGGGANLAVDVVGYFAAPRSDGEYFLQGGNAFGA
                     TATLGTTDNQPVSILAGNAPGLRLVPATDSSGGVSINVIGGFSSNAIDAGVIGATIGG
                     GGAHFGNGQIGVNHVSASVGTIGGGTGNAVTGESGTVSGGEVNTAGPLATVGGGIANT
                     ASGINSTVAGGVANTASGGSSVVMGGDGNTASGEESFAAGFLANANQNGCILFAYWHP
                     GSGGGADCFG"
     gene            complement(1451..2029)
                     /locus_tag="VFO53_00365"
     CDS             complement(1451..2029)
                     /locus_tag="VFO53_00365"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HET8874753.1"
                     /translation="MGRKASASGIRRQLAAFTIVALGISAMPALAAVDSFIKIAGIDG
                     ESQDEQHKGEIDVLSWSWGMSGVSSRASSTNPSQPVGPLARACAREFTITKQVDKASP
                     SLFANAATGTPIQNAVITVRKAGRGQQEFMTITLSNVVVSSVAQVGASDDSGPTESVS
                     FNFSSVLITFTPQNADGSAGDPIKAPAPGSCP"
     gene            complement(2146..>4287)
                     /locus_tag="VFO53_00370"
     CDS             complement(2146..>4287)
                     /locus_tag="VFO53_00370"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET8874754.1"
                     /translation="NLIVHRDIKPGNILVARDGDVKLLDFGIAKLLEDDIEAGATQLT
                     REGGRLLTPEYAAPEQLTGQPVTTATDVYALGVLLYALLAGRHPLGSTTQAPAQLIRA
                     LVGSPPARLSAPGRTDPSLDAATIAAQRGLTPERLRQRLRGDLDSIVAKALEKDPRDR
                     YASVAAFADDVRRHLSEQPVRARADSFRYRAVKFVRRNRIPVLLSSLAAVATLAGLAG
                     TIVQAQRATAQRDFALRQLARAAALDDFNGFLLYGVAPAGKAFTSGELMARAERIVVR
                     AAPDAMRTDMLVAMGGQYLAMDHDADARRLLVEAYRVSRGDGVTHARAACNLSKALAR
                     QEGDRDESLRVFDEAMAALPNEPQYLFDRIGCLLTGSDAARAIGEARLGVERAEAAQR
                     LIARLPYPSKAVELRAGIELAESYRVAGDFAKAEDAFAAVYQKLKDLGRDETQQAGSL
                     LNNWGVALGQLGQPFRAEALLRKAIDISRDDDTGAGVSPMLLTNYARVLSDLDRDDEA
                     VRYIDRAYAEAKREDNELVTNMALSVQAVIRRKRGDFDAAERSLDEVTPRLRRTLPSD
                     HFFFALALSERSLIAAGRHENAAAIELANRAVDGLNSRNSGNPLAESVALVRRAKLEL
                     DMGRAVDAEADAARALALEQRSLVSGHASSLLGRTYLALANAEAANGRHDDAQRAFAA
                     AADQLRPTLGADHADTQLAERLAAAGPRSGH"
BASE COUNT          689 a         1488 c         1392 g          718 t
ORIGIN      
        1 tccgaagcag tcggcgccgc caccgctccc cggatgccag taggcgaaaa ggatgcagcc
       61 attctggttg gcgttggcga ggaaacccgc tgcaaatgac tcttctccgc ttgcagtgtt
      121 gccgtcaccg cccatgacca ccgagctgcc gccgctcgcc gtgtttgcga ccccgcctgc
      181 caccgtcgaa ttgatgccgc tcgccgtgtt ggcaatgcct ccgccgaccg tcgcgagcgg
      241 acctgcggtg ttgacttccc cgcccgatac cgtacccgat tcacccgtga ccgcattgcc
      301 cgtgcccccg ccgatcgttc cgacgcttgc cgacacatgg ttgacaccga tctgaccgtt
      361 gccgaaatgc gccccgccgc cgccgatcgt tgcgccgatg acgccggcat cgatcgcatt
      421 cgacgaaaag ccgccaatca cgttgatcga aacgccgccg ctgctatcgg tggccgggac
      481 caggcgcagg ccgggcgcgt tgccggcaag gatcgatacc ggctggttgt cggtggtacc
      541 gagcgtggcc gtcgccccga aagcgttgcc gccctgcagg aagtattcgc catcgctgcg
      601 cggcgccgcg aagtagccga cgacgtccac cgcgaggttc gccccgcccc cgcgcacctg
      661 cacgctgatc tcgttgtcgg cgaggttgac cttggcgctg gtcgacaccg tgttgaacgc
      721 gatgctgccc acgtacacca tcgtcgccgt gcttccgaag ctcgctccct gcggcaggat
      781 ctcgatgtcc cccgatgcgc tcgtcgtcgg gatgccgaac acctgcaatt gcaccgcgct
      841 cgggtcgagt cccgccggca gttgggtaag gcacacgccg ttgccgccct gcaacttata
      901 gctgcgtatc tcgctcggcg cgaacggcag cgcatgatgc gacgcgctgc cgtcaccttg
      961 atagacggcc gcgaacgtcc cgcgcgtttc gaccagccgg cacggcgtca ccggcgtgta
     1021 aaccgcgccg cttccggcgg gcgcgagttt cggttgcccc ggcgccggct tcgcaagctc
     1081 gtcgacaccc agcacggcgc gcatgccctg cagtgtcccg gtaagactcg ccgcgagcaa
     1141 ctggtcggcg cgcaggccca tcagccggtc gcgcagatcg tcgacgccga tcggcgcact
     1201 cgacttggca agcggcccgc cccattgggc gacgatgcgt tcgacgacgc tcgcccgctg
     1261 ctcgtcgatc gccagcagcg cgtccgccgc gcgcagcggc tgcgcgacgc acgtgctggc
     1321 gaccagaccg acccatacca tcgacaacgc ccgacgcaac acggcacgca agcttcgcac
     1381 gatgctctcc ttcagggacg cgtctcgacg ccgccagttc agcagggtcc gtcaacgaaa
     1441 ctcacgatcg ctacggacaa cttcccgggg caggcgcctt tatcggatcg cccgcactgc
     1501 cgtcggcgtt ctgcggcgtg aacgtgatca acaccgagga aaagttgaac gagacgcttt
     1561 cggtcggacc gctgtcgtcg cttgctccca cttgcgcgac cgacgaaacg acgacgttgg
     1621 aaagcgtgat cgtcatgaat tcctgctggc cccgcccggc cttgcgcacc gtgatcacgg
     1681 cgttctgtat cggcgtaccc gtcgccgcgt tggcgaacag cgatggcgac gccttgtcga
     1741 cctgcttggt aatcgtgaac tcacgcgcgc acgcacgtgc aagcggtccc accggctgcg
     1801 atggattggt cgagcttgca cgcgaggaaa ctccgctcat cccccacgac cacgacagca
     1861 cgtcgatttc gcccttgtgc tgctcgtcct gcgattcgcc gtcgatgccc gcgatcttga
     1921 tgaagctgtc gacggctgcc agcgccggca tcgcggaaat gccaagcgct acgatcgtga
     1981 atgcggccag ctggcgacga atgccgcttg cgcttgcttt tcgacccatg acgttctccc
     2041 aggtagttgc ggttccttgt ggtcccgctg cctggtaaat cgaaaatggg cgggaaacac
     2101 ggccaccccg gccgacggac ggcgggggtc gcgcggttgc gacggtcagt gaccggaacg
     2161 ggggccggcg gcggcgagcc gctcggcaag ctgcgtatcg gcgtggtcgg cgccgagcgt
     2221 cggccgcaat tgatcggcag cggctgcaaa tgcccgctgc gcatcgtcgt gccggccgtt
     2281 cgccgcttcg gcattggcca gcgcgaggta ggtacggcca agcaggctcg aggcgtgtcc
     2341 cgacaccagc gaacgctgct ccagcgccag cgccctcgcc gcatcggcct ccgcgtcgac
     2401 agcgcgtccc atgtcgagct cgagcttcgc cctgcgtacc agggcaactg attcggccag
     2461 cggattgccg gagtttcggc tgttcaagcc atcgaccgca cgattggcga gctcgatcgc
     2521 cgccgcattt tcatggcgcc ccgcagcgat cagcgagcgc tccgacaagg cgagcgcgaa
     2581 gaagaagtgg tcggacggca aggtcctgcg cagccgcggc gtcacttcgt cgagtgaacg
     2641 ctccgcggcg tcgaaatcgc cgcgtttgcg ccggatcact gcctgcaccg aaagtgccat
     2701 gttcgtgacg agctcgttgt cctcgcgctt cgcttcggca taggcgcgat cgatgtagcg
     2761 aaccgcctca tcgtcgcgat cgagatcgga cagcacgcgc gcgtaattcg taagaagcat
     2821 cggcgacacg ccggcgcccg tgtcgtcgtc gcggctgatg tcgatcgcct tgcgcaacaa
     2881 cgcttcggcg cgaaacggct gcccgagctg gccaagtgca acgccccagt tgttgaggag
     2941 gctccctgcc tgctgcgttt cgtcgcggcc cagatccttg agcttctggt aaacggcggc
     3001 aaacgcatcc tcggcttttg caaagtcgcc tgcgacacga taggactcgg cgagctcgat
     3061 ccccgcgcgc agctcgacgg ctttggacgg gtacgggagt cgtgcgatca ggcgctgcgc
     3121 cgcttcggcg cgttcgacac cgagtcgcgc ttcgccgatt gcgcgcgccg cgtcgctgcc
     3181 ggtcagaagg cagccgatgc gatcgaagag atactggggc tcgttcggca acgccgccat
     3241 tgcctcgtcg aacacgcgca acgattcgtc acggtctcct tcctggcgcg caagtgcctt
     3301 ggacaggttg cacgctgcac gcgcgtgcgt cacgccatcg ccgcgcgaca cccgatacgc
     3361 ctcgaccagc aggcggcgag cgtcggcgtc gtggtccatc gcgagatatt gcccgcccat
     3421 cgcgacgagc atgtcggtgc gcatcgcatc gggcgctgcg cgaacgacga tccgctcggc
     3481 gcgtgccatc agctcgccgg aagtgaaagc cttgccggcg ggcgcaacgc cgtaaagcag
     3541 aaagccgttg aagtcgtcga gcgcggctgc ccgcgcgagc tggcgcaacg cgaaatcgcg
     3601 ctgtgccgtt gcgcgctgcg cctgcacgat cgtccccgcg agcccggcga gcgtcgcgac
     3661 ggccgcgagc gacgacagca gcaccggaat ccgattgcgc cgcacgaact tgaccgcgcg
     3721 atagcgaaac gagtcggcgc gcgcgcgaac cggctgttcc gacagatggc gccggacgtc
     3781 gtcggcaaat gcggcgaccg acgcataacg atcgcgcgga tccttctcga gcgccttggc
     3841 gacgatgctg tcgaggtcgc cgcgaagccg ctgcctcagc cgctccggtg tcaggccgcg
     3901 ttgcgctgcg atggtggcgg cgtcgaggga cggatcggtg cgccctggcg cggaaagccg
     3961 cgcgggaggc gagccgacga gcgcgcgaat gagctgcgcc ggagcctgcg tggtgctgcc
     4021 cagcggatgt cgtccggcga gaagcgcgta gagcagcacg ccgagcgcgt agacgtcggt
     4081 cgccgtcgtc accggctggc cggtgagttg ctcgggcgcc gcatattccg gcgtgagcag
     4141 ccgcccgccc tcgcgcgtca attgggttgc gcccgcttcg atatcgtcct cgagcagctt
     4201 cgcgatgccg aaatccagaa gcttcacgtc gccatcgcgc gcgacgagaa tgttcccggg
     4261 cttgatgtcg cgatggacga tcaggtt
//
LOCUS       DASSVX010000007        29399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_750052, whole genome shotgun sequence.
ACCESSION   DASSVX010000007 DASSVX010000000
VERSION     DASSVX010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 29399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29399
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750052"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VFO53_00375"
     CDS             complement(<1..376)
                     /locus_tag="VFO53_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874755.1"
                     /translation="MDDSIRRIAHFRPDCATPRNKRNKTGRNALQSSPWARPKKTKQM
                     CPAIETALPHRLPRLHRLPGLAGSRVRRGSFSGAAGGAKTERTSAMYPTPRKSREHVA
                     APTSEADDLLDLIAGPVTAEMAV"
     gene            453..1163
                     /locus_tag="VFO53_00380"
     CDS             453..1163
                     /locus_tag="VFO53_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8874756.1"
                     /translation="MSPNTTILVVDDEPDVREVLHDYFVAHGYAVVGAGSAEAARAEA
                     ARQPIDLALVDIHMPGEDGLALARHMRERYAKMAIIMLTSASTVVDRIVGLEMGADDY
                     VPKPFDPRELAARVKSVLRRTSYASRADIGASRVRIGRCVLDLGAHRLTDDEGTEVAM
                     SPLEYDLLKAFAEHPNRALSRERILNLNDQRDWDPFDRSVDLRIMRLRKKVEPDPEHP
                     RFIRTIRNEGYIFVPDGR"
     gene            1171..2163
                     /locus_tag="VFO53_00385"
     CDS             1171..2163
                     /locus_tag="VFO53_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8874757.1"
                     /translation="MNRPWLAPPRIRRAVHLLACGALVLSLFGCMGPPESALRIGTNV
                     WIGSEPLYLARDLGRLDSDHVQLVEYPSASEVLRAYRNQAIDGMVISLDELFGLAVDG
                     LQPRIVLVVDVSNGADVVVGRAGMRSMRDLKGKSVAVESGALGAFVLSRALALNGMQA
                     SDVNVVHLESNEHPGAFDKGQVDGAVTFDPYRAQLLAQGASTVFDSTQIPGEIVDLLA
                     VRASVVDRHPKTIQALLAGWFAALDYMKREPADAAQRMGIRQQTSGEQFLEAQKGLHI
                     PTREENLRMLGGPKPELATAGRRLMALMLDAKLLHADVNIESVLAPQPLLDLPR"
     gene            2160..4859
                     /locus_tag="VFO53_00390"
     CDS             2160..4859
                     /locus_tag="VFO53_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET8874758.1"
                     /translation="MSLRVPASLKITVPLLLLAFAATLSAVNLVYHVPQAERGAEEES
                     RTRLAQEMSRLQSTLEYLLLKGDAEAAQHEIAVLAHNHDVLLAALTDERDMIIAATRR
                     AWLGRQIEDVSPQFDADQAARAIRERRAGIAIGLNGDVLLGHAGILMGSERDELRPSR
                     TGSLFLAYDLKRYKAEARAQVVQQSLYWAGWVTALALALWLVFHFLLTRRTARLVDAA
                     EQLAAGNLGARSDLGGQDELGRLGRAFDTMARKVAGTQTRLREDIAARERVQRELEMS
                     EASYRAIFDAAEDAIFVHDIDTGAIVDVNPKACATFGYERDEFRRLEIGTLGTGEHPY
                     AQKDAMALIARARAGEQLRVEWRGKAKSGSLRWYDVFSRRVTIGGHDRILSLARDITD
                     RKRAEAALRASEEQYHAMFNASIDGLALWNAAGEVVDINPALWRMYGHTDAEFSAMSA
                     QHWPRPSYQPEFLRVVGAGESLHSEVTESRKDGSALQLEVHGIPMQYQGRPHVLTIAR
                     DITGKKHAEEELARQRESLYQREKLAALGSLLAGVAHELNNPLSVVVARAVLLEEQGD
                     AATQAAALKIRTAAERCARIVRTFLAMARQQQPERGPVAINDVITAALDMTGYAIRTS
                     GIDVSLALASDLPLILADADQLHQVLLNLIINAQQSLQEHPAPRRISVTSSLDAGGET
                     LRIAVADNGPGIAPQHRARIFDPYFTTKPTGMGTGVGLAVSLGIVESHGGTLGVACPR
                     EGGAIFTVALPVRHADVGEAPAAPEPAPSPRRRSVLVVDDEAGIRESLAEILCGARHR
                     VVTASSGREALERMAVEYYDVILTDVRMPDLDGRALYAQIEQRWPRQAARVVFVTGDT
                     LASELREFVRNSGRRVIEKPFLPSEVRRIVAELAADAELSRPG"
     gene            4989..5243
                     /locus_tag="VFO53_00395"
     CDS             4989..5243
                     /locus_tag="VFO53_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874759.1"
                     /translation="MSFDECSTPRAALGLLGIAMAAITMGALVVLPAKLEAASGGVTG
                     VTAATTAAGAGPAAAGNAHDERFDARVSGDSNCNSTRDRS"
     gene            5240..5662
                     /locus_tag="VFO53_00400"
     CDS             5240..5662
                     /locus_tag="VFO53_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008030796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET8874760.1"
                     /translation="MNYEGSCFCGAVGLAVTGEPVAEGYCHCASCRSWSAAPLNAFTL
                     WKPDAVKFTRGEDNVGVFHKTDRSYRKFCRTCGGHLLTDHPQWALVDVYAATIPAHRH
                     RPALHVNYAESVLPIRDGLPKMKDLPKEMGGSGETCPE"
     gene            complement(5727..5969)
                     /locus_tag="VFO53_00405"
     CDS             complement(5727..5969)
                     /locus_tag="VFO53_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874761.1"
                     /translation="MFHIPAVTWGQPSPALRDLFLKALDGRDRAAIEASARNVHGCAF
                     SLPGVTCVLLGLAPGSTYGDAAAMIAASNGHPFDRG"
     gene            complement(6118..7746)
                     /locus_tag="VFO53_00410"
     CDS             complement(6118..7746)
                     /locus_tag="VFO53_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HET8874762.1"
                     /translation="MTEVLYTVAALLAIVGAVAWIAARLHLPPPILLVVAGIALALMP
                     WVPSITLHPDVVLLVLLPPLIYFAAFTMSWQAFRENLRPILLLAFGCVIVTTVAVAGA
                     AHWLLGFPLGVAFMLGAIVSPPDVVAPLAVAERLSIPRRIIAILEGEGLVNDATALIL
                     FNLALTAVFTGRFSLPEALGDFALVLVAETAWGFVVGYVILRLRHFAAEPRLEVLLSL
                     LTPFVAFWPPHALGGSGVLATVVSGLYVGFAGAALIRSNTRLQALFFWDVTNTVITGT
                     IFLLTGLQARTVAAGLDTATLTHLVIDGLLVSAVVIGVRFLWVFPATYAPRWLFPRIA
                     ARYPAPNWRMPFVIAFTGVRGVVSLAAALSVPIAIASDVPFPRRDGVLVLTFVVIVVT
                     LVAQGLTLPWVLRKLRLDKLGRAELRRRKEREIYARVEAARAALAELDAAARTYALPE
                     DVIARWREREQLRITQLEGERHGNGERFAASASMHRLDLALVAAQRKRLNELLRSGEV
                     SDAIRRRVERDLDLDEERLRGSLQGLASNGEDAV"
     gene            complement(7864..8262)
                     /locus_tag="VFO53_00415"
     CDS             complement(7864..8262)
                     /locus_tag="VFO53_00415"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HET8874763.1"
                     /translation="MAHGVPVQWRNDMAKYGKKAQAKVRKVMHERKEGTLRSGGSGRK
                     VKSRKQAIAIGLSEARRAGAKVPKKTAAKKSASKQSASKKKPAKKSSPRKKSASRKSP
                     AKKSASKKSAAKKSPSKKSAAKKKSAKRSS"
     gene            8320..10026
                     /locus_tag="VFO53_00420"
     CDS             8320..10026
                     /locus_tag="VFO53_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET8874764.1"
                     /translation="MNAAHDAFIAARDFLLAHRTDYDTAIRDFHWPALTQFNWALDHF
                     DVMAAGNDQPALWIVGDDASERKRSFREMSERSSQVANHLRALGVQRGDRILLMLGNE
                     VALWETMLAAMKLGAVVIPATALLTAADLRDRINRGRVRHVVAAHAHTQKFAEVEGDY
                     TRICVGTPCEGWVSHDAADAFAREFAPDAPTLATDPMLLYFTSGTTSKPKLVLHTHQS
                     YPVGHLSTMYWIGLQPGDVHLNISSPGWAKHSWSCVFAPWNAGACVFIYNAARFDARG
                     LLAAIERCKVTTLCAPPTVWRLLIQQDLAAYRGRLGIRELAGAGEPLNPEIIGQVRDA
                     WGITLRDGYGQTETTALLGNSPGQPLKPGSMGRPLPGYRIALLDVEGREAADGEVCID
                     LGVHPLGLMRGYEDSEEATAQSMRNGYYHTGDTAARDADGYVTFVGRADDVFKSSDYR
                     ISPFELESTLLEHACVTEVAVVPSPDALRLAIPKAYVTLAAGSEPSRELARDILAFAR
                     QALAPYKRVRRIEFVVELPKTLSGKIRRVELRKAEALRREQGVRAPDEYFEEDFPDLK
                     RG"
     gene            10124..11422
                     /gene="eno"
                     /locus_tag="VFO53_00425"
     CDS             10124..11422
                     /gene="eno"
                     /locus_tag="VFO53_00425"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET8874765.1"
                     /translation="MNTTTIRALHAREILDSRGRPTVEVDAMLDDGSVGRASVPSGAS
                     TGAAEAHELRDGDPARYGGLGVLRAVANVNTEIDRALRGVDGADQASVDARLRELDGT
                     PRLCRLGANATLGVSLAVCRATAVAQRQALYERIAELAQTQPLLPLPMTNILSGGWHA
                     GGGMDVQDFLVVPMRATSTGEAIHAIARVRTAASDVLRKRGLPTLLADEGGLGPGCRT
                     SHAALELMIESIERAELAPGIDMAIAVDVAASTLLQADGRYRLPREGRECTREAMIDM
                     IDGWVREFPLVSIEDPLAEEDWPGWTALTRRVGGVLQLVGDDLFATNCARVARGIGEG
                     IANAVLVKANQNGTLSGTLDVIAQAKRAGYATIVSARSGETEDAFMSDLAVGAAAGQI
                     KIGSLRTSSTVAKYNELLRIAERSGARYAGAAALAPARHR"
     gene            11419..12435
                     /locus_tag="VFO53_00430"
     CDS             11419..12435
                     /locus_tag="VFO53_00430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012483918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET8874766.1"
                     /translation="MTVQRTPDALLIEFVRRTGLAGDAETPVFTPLEGGVSSDIWRVD
                     LAQRSICIKRALPRLKVRQAWTAPIERNRYEWAWVEFAAAHFPGCVPEPIASDPVHGI
                     FAMAYLDPGRYPLWKRRLLDGIAESGVAAAVATTVARIHSASAGDAGVARRFATDAIF
                     HAIRLEPYLLATAVRHPRLAAPLRALAQRTAGTRLALVHGDVSPKNILLGPRGPVFVD
                     AECAWYGDPAFDAAFCLNHLLLKCIARPGSCNDYLACFEAFAHAYFAGVTWEPRAAIE
                     SRIAGLLPALLLARIDGKSPVEYIRDERDRQRVRDAACPLIERSPQHVADVATAWHRM
                     MAAP"
     gene            12583..13575
                     /locus_tag="VFO53_00435"
     CDS             12583..13575
                     /locus_tag="VFO53_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8874767.1"
                     /translation="MKKSSIAAWLTGTLLAAAVAAPLAAQPGGKLVLYTSQPDRDAQR
                     TVEGFRKKNPAVEVEIFRSGTTEVMSKLMAEIAAGAPRADVLLIADALSMDRLKAAGE
                     LQAYPGADVRELPPDAYDKAKTYFGTKLITTGIIVNRQATMKPTSWKDLLKPEAKGQV
                     ALPSPLYSGAAAIHMGALHASPALGADFYEKLAKNGAVALRGNGAVLNAVAGGQKMYG
                     IIVEFMALNARAKGSPVDFVFPAEGVSIVTEPTAILRGTKNPEAARAFVDFLLSKDGQ
                     ELAVSQGYFPARKDVKPPPGFPDVASLRILPVDISELEKRDELNKKRFAELFGG"
     gene            13588..15285
                     /locus_tag="VFO53_00440"
     CDS             13588..15285
                     /locus_tag="VFO53_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET8874768.1"
                     /translation="MPLIPARRGERLTFATLLGLVVLLAVVPIARLAQEALAPHGRLD
                     AGVLASVVESPVVWRAARNTLSVSVGGAALAIVLGTAFALLVSLTDVRAKKPLVFCFM
                     MPLLIAPQVTALAWINLLGPNSTLLRVLGIAPAAGSPHPLYGPGGIVLLLGLEQAPVV
                     FLAVRAGLRSLPRDLVEAAQAAGARPWRIVRTIVLPLMMPSVAAGGMLAFVGSIGNFG
                     IPAFLGIPAGFTVLVTLIYQRLAGFGTSVLSEVAALSLLLALIALCGLALNGWLARRR
                     DVRVSAWATATPQWSLGRMRFAIEGTCWSALFAVLALPFCALVATALVPAIGVSLTPQ
                     TLTFGNFHFVLFDYAATRRAFVNSASLAAAAAVMLVIVSVPLGYSLAGRRASVRQAVG
                     FVAELPYAVPGVVLAIAMILCLLRPLPLLGLSLYSTIWIILVAYLSRFMLLALRPVTN
                     ALRQIDPSLDEAARMSGARYARRVATVIMPMIAPSAVAGGMLVFLMAFNELTVSALLW
                     SSGSETVGVVLFSLEQAGDAVSAAAVAVIAVVVTLGMMLMANALASRVARGVLPWQP"
     gene            15273..16337
                     /locus_tag="VFO53_00445"
     CDS             15273..16337
                     /locus_tag="VFO53_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8874769.1"
                     /translation="MAAVTLAGIGKRFGSIAAVEDVSLGVEEGEFLAVLGPSGCGKST
                     LLRLIAGFERPERGTIRLGDRLVSGPEDYVAPEDRHVGMVFQSYALWPHMSVAENIGF
                     PLRVQKLSGRDLARQVGAALDAVGLSGLGDRRPAELSGGQRQRVALARCLAMKARIVL
                     LDEPLANLDVHLRARMLAEIAAFRTLSKATLVYVTHDQAEALGVADRVAVMAGGRLLQ
                     VASPPALYRTPQDGIVSRFVGPGAVARGEVTQTYANGHAKVALFGAEWQVRSAPGQGT
                     GPAEICIRPEDLAVVAPGGGFEASVARVLYQGGRTIVEAAPASDPATLLSLSLESSEP
                     LPPGGTVHLALVDGWVIPGR"
     gene            16334..17335
                     /gene="hpnA"
                     /locus_tag="VFO53_00450"
     CDS             16334..17335
                     /gene="hpnA"
                     /locus_tag="VFO53_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HET8874770.1"
                     /translation="MTRANLVLLTGATGFVGSAIARALVAAGYPVRALVRAGAVRNNL
                     AGVPLETFDGDICDEAALARAMSGVRYVVHAAADYRLWARDPSALVRTNVDGTRLVMQ
                     AALRSGAERVVYTSSVATLATRATGDAADETAPLDERAAIGAYKRSKVAAERVVEAMV
                     ARDGLPAVIVNPSTPVGPRDIKPTPTGRIIVEAARGRVPAYVDTGMNLVHVDDVADGH
                     VAALTRGRVGERYILGGDNVSLVTMLSDIAQLAGGRAPRWKLPRGPLYPLAAVAEAYA
                     RATSREPFLTRDGLRMSEHRMFFTSAKASRELGFRARPYAEGLRDAVGWFRAAGYLR"
     gene            17332..18507
                     /locus_tag="VFO53_00455"
     CDS             17332..18507
                     /locus_tag="VFO53_00455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8874771.1"
                     /translation="MSVAIAALGCVAWLYLLAARGGFWRAGDRDDAPAAIADLDAWPR
                     VTAVVPARDEADVIGANLASLLRQDYPGAFSVIVVDDHSSDGTAAVARAAAGDGTASR
                     VTVLPAPAVEPGWTGKLWALRHGIHHSEALPASSDYLLLTDADIAHAPDTLRMLVSRA
                     RRERLLLSSLMAKLDVQSAAERALIPAFVFFFRMLYPFAWVNEPRCATAAAAGGCMLV
                     DRNALRAAGGLAAIRGELIDDCALARLLKPHGPIRIALTDRVRSTRAYRSVGELRRMI
                     ARTAFTQLRFSVWRLLATVFALLVVFVAPPLLALPSADPLVRTLGGLGWACMTLAFVP
                     MLRFYRRSPAWALALPAIAAAYLAFTIDSAFQHLRGRGGEWKGRVRPRAALTPASRR"
     gene            18551..20530
                     /gene="shc"
                     /locus_tag="VFO53_00460"
     CDS             18551..20530
                     /gene="shc"
                     /locus_tag="VFO53_00460"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011548765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HET8874772.1"
                     /translation="MRNPEHIARRRRGDERDTRVDAAIDEATAALLDAQRADGHWVFE
                     LEADSTISSEYITLMHFLGERDAPLEARIARYLRRRQAAHGGWPLTHGGPFNMSASVK
                     AYYALKLAGDSADAEHMRRARDAILAHGGAGTANVFTRILLALHGILTWSSVPVVPVE
                     IMLLPRWFPFHLSRVSYWTRTVLVPLLVLQAKKPRARNPRGVRIDELFPNGRPHARRW
                     ARAPHQARIPYAVFAALDVLLHGIERLIPRGLRQRAIERALAFVAERANGEDGLGAIF
                     PAMANAVMMYDTLGYARDREPYATARKAVDGLLVVSGDEAYCQPCVSPVWDTALACHA
                     LLDAGDPAAIASVHRALSWLAERQVLTVAGDWKDCRPGVRPGGWAFQYANAHYPDLDD
                     TAVVVMAMDRWLHLHGVHPEPRLRETIRRAHEWVLGMQSSNGGWGAFDADNVSEYLNH
                     IPFADHGALLDPPTADVTARCAGMLAQLGGATDAPAIASALAFLRRTQEPDGSWYGRW
                     GMNYVYGTWSVLCAFHAAGVSPQSLDVRRAVDWLSSVQNADGGWGETAQTYELGYRGD
                     KRGPSTASQTSWALLGLMAGGETRNQAVARGVDYLLRTQGDDGFWPEPEFTATGFPRV
                     FFLRYHGYAKFFPVWALARYRAQLSSSLPRMRLGM"
     gene            20527..21246
                     /locus_tag="VFO53_00465"
     CDS             20527..21246
                     /locus_tag="VFO53_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HET8874773.1"
                     /translation="MSSPAAQPAHAGFVLVACGLALEARIASGPGVRSIAGGVDRQRL
                     ARELEDAVTAGAEAVISFGIAGGLAPGLRAGTRIIGRGVHAAAGYRACDAAWIARLAE
                     RLPGALVGDLAGADRPVANADAKRALHAATSALAVDTESHVAADVAVTHGLPFAALRV
                     VADAAFRSLPEAALVGLDAGGTVDVAAVLRSLARTPSQLPLLLRTACDARVAFAALRR
                     GRRCVGVRFGCDLRALDLDVT"
     gene            complement(21172..22317)
                     /gene="hpnH"
                     /locus_tag="VFO53_00470"
     CDS             complement(21172..22317)
                     /gene="hpnH"
                     /locus_tag="VFO53_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HET8874774.1"
                     /translation="MAIPLLQKTRIGAYVMRQQLAGRRRYPLVLMLEPLFRCNLACAG
                     CGKIDYPGEILDRRLSVAECLEAVDECGAPVVSIAGGEPLLHKEIVDVVRGIVERRRF
                     VYLCTNALLMKKRLAKFTPSPYFVWSVHLDGDRSMHDKSVCESGVYDRAVAAIGEAKS
                     RGFRVNINCTLFNDAEPERVAAFFDSVAALGVDGITVSPGYAYERAPDQAHFLTRTRT
                     KQLFRDILSRGRGGRAWSFSQSSLFLDFLAGNEEYRCTPWGNPTRNVFGWQRPCYLLG
                     EGYAKSFRELMDETDWDAYGTGNYEKCANCMVHSGYEATAVTDAVRHPLKALAVSVRG
                     VRTHGPMAPEIPLGQQRPAQYVFSRHVEIERAKIASEPHANATAPAP"
     gene            complement(22334..23281)
                     /gene="ispH"
                     /locus_tag="VFO53_00475"
                     /gene_synonym="lytB"
     CDS             complement(22334..23281)
                     /gene="ispH"
                     /locus_tag="VFO53_00475"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET8874775.1"
                     /translation="MRVMLAQPRGFCAGVIRAIDIVEQALERFGAPVYVRHEIVHNRH
                     VVESLRAKGARFVEHLSEVPVGSITVFSAHGVARVVEDDARTRGLRVLDATCPLVAKV
                     HGQGKRYVSKGRTVILIGHAGHPEVEGTMGQIPGPVLLVQNEQDVERLELAPDTPVAY
                     VTQTTLSVDDTRSVIAALARRFTNIVGPDIHDICYATQNRQTAVRELARVADVILVVG
                     ARNSSNSNRLREIGVESGVPSYLIADGTELDPAWVKAAEVVGVTAGASAPETLVDGVI
                     DALRALGPIEVSTLPGIEEKVEFRLPAELLRKHAGHGAT"
     gene            23397..26042
                     /locus_tag="VFO53_00480"
     CDS             23397..26042
                     /locus_tag="VFO53_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET8874776.1"
                     /translation="MTDPTPEHRRRDARGPDRGLVSPIRKTVIACAAHPLPVIVTAFL
                     LCAAALVHVMTHFAIDTDASKLISADVPWRQRELAFAAAFPQRVDLIAVVVDGTTPEQ
                     ADTATARLAERLAGERALFRAVWRPDGGAFFEREGLLFESTQEIAQTTQRLIAAQPLL
                     GTLAADPSLRGLMDALSLVAMGAARDAAALDAMGKPLAALATALEAIAAGQAPAFSWH
                     ALFTGEAPDPRLLRRFILVQPVLDYAALQPGERATAAIRTAARQLHLDAVEGVRVRLT
                     GPVPLADEEFSTLAEDAGLNATVTLGVVVALLWIALRSWRLIVAILVSLAIGLIATAS
                     FGLLAFRAFNLISVAFAILFVGLGVDFGIQFSMAYRARLHATGDSRHALRDIGGEVGA
                     ALALAAAATAAGFFAFVPTDYRGVSELGVIAGTGMIVAFAASITVLPALVAFLRPRAE
                     RAEVGYPGLAPLDRWLVVHRRIVLAAGAAVALAGLALVPKLVFDFNPMHLRSPEAESV
                     ATLLDLMKDSATAPDTIDVLAPSLDAAVGLGERLAQLPEVDHVLSLASFVPEAQDEKL
                     ALIQDAALLLEPTLAPDSIKAAPTDAAAVAAMSHAAQRLDEARAGRSGMPFATTAARL
                     AGALRALADGPPAVRERARTLLIPGLQTALAQLRNALQAAPVTLASLPGALRHDWISA
                     DGRARVEVYPKGDVSENAALRRFVDAVQAVAPGATGAPVSIDASSRTIIGAFVKAGVL
                     AFVSITLLLVLTLRRAQDVVRTLLPLLLSALGTLGSCVIIGLPMNFENIIALPLLFGI
                     GVAFNIYFVMAWRAGRPNPLQSSLTRAVVMSGLTTGTAFGSLWLSHHPGTASMGELLA
                     LSLAWTLVAALLFLPALLGEPRQRP"
     gene            complement(26069..27040)
                     /locus_tag="VFO53_00485"
     CDS             complement(26069..27040)
                     /locus_tag="VFO53_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET8874777.1"
                     /translation="MKRAARVLAIAGLALAAGLFAHENMTRIALLVAGAIPGLLLAAS
                     FHVVPMVTNALAWQWLFPQPMRPRLRVLLNAVWIRESVNGVLPVARVGGEIAAYRVLR
                     RHVASRAAVAASLAADVALSVLSQSAFALLGLAMLVIAGHGGAFTRELAFGAAALLAA
                     GGALLLAQRVGALASLAGVAERLFSGRLRSARARSLRIDRALRDVHARHRDVVVCVVL
                     QFAAWVVSAGETWLALHFLGAPRSVHEAIAIEATVQAINSVAFVVPAAIGVQEGAFVL
                     IGGVLGLDATTALALAAARRLRDVVFYVPGLFAWHRAERSATACRHA"
     gene            complement(27037..27897)
                     /gene="hpnK"
                     /locus_tag="VFO53_00490"
     CDS             complement(27037..27897)
                     /gene="hpnK"
                     /locus_tag="VFO53_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HET8874778.1"
                     /translation="MGIVQGFPRKLIVTADDFGAAREVNEAVELAHTRGILTAASLMM
                     SGAAVDDAVARARRLPALRVGLHLVLVDGAPVLPRHAVCDLVGADGRFPADLARAGRN
                     IFFSPSARRQLRAEIAAQFDAFRATGLALDHVNAHHHFHLHPTVAALVLEIGATRGVR
                     GLRVPREPAGVLARIEPSQHPHGDWLAKLCTRLLARRTRRRGVMTPANVFGLAWSGAM
                     TRPRLEGLLHHLPDGVSEIYAHPATSDGFAGASGGHAEELAALVAPSVVKLLRGSDIA
                     TGGYSDFIAA"
     gene            complement(27908..29335)
                     /gene="hpnJ"
                     /locus_tag="VFO53_00495"
     CDS             complement(27908..29335)
                     /gene="hpnJ"
                     /locus_tag="VFO53_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HET8874779.1"
                     /translation="MKTLFLHPPSFEGFDGGAGSRYQARREIRSFWYPTWLAQPAALV
                     EGSKLVDAPPHGLALSDVLATARDFDLAVLHTSAPSFASDVKTIEAMKGVNRDLKAGL
                     IGAKVAVDPEGSLAASAAIDFVAGNEFDFTIREVAEGRDLDTIAGLSWRNQAGRIVHN
                     PPRAPLANMDALPFVTPVYKRDLEIERYFIGYLKHPYVSLYTGRGCKSRCTFCLWPQT
                     VGGHRYRTRSVGHVIEEIAWAKQALPQVKEFFFDDDTFTDDLPRTEAIAKELGKLGIT
                     WSCNAKANVPRETLKVMRDNGLRLLLVGYESGNQQILFNIKKGMRIEFARRFTKDCHD
                     LGITIHGTFILGLPGETKETIEETIRFATEINPHTIQVSLAAPYPGTYLYRQALENGW
                     LDDAHAELVDAHGVQIAPLHYPHLSHTEIFDSVETFYRRFYFRSGKIASIVGEMVKSP
                     QMMKRRLREGVEFFQFLRERRGTAQ"
BASE COUNT         4421 a         9986 c        10227 g         4765 t
ORIGIN      
        1 atacggccat ttcggcggtg acgggccctg cgatcaggtc gagcaggtcg tcggcttcgg
       61 aggtcggcgc ggcgacgtgc tcgcgcgatt ttctcggagt cggatacatc gcggatgtcc
      121 tttcggtctt cgcgccgccc gcagcgccgg aaaacgaccc ccggcggacg cgactcccgg
      181 cgaggccggg caagcgatga agtctaggca ggcgatgcgg cagcgccgtt tcaatggcag
      241 ggcacatttg tttcgttttc ttcgggcgag cccacggaga ggattgcaaa gcattcctgc
      301 cggttttgtt tcgtttgttt cggggcgtgg cgcaatccgg gcgaaagtgc gcgatccgcc
      361 ttatgctgtc gtccacgcgg tctcgattcc tggcagattc ccgccggcgg acgaagaagt
      421 ccggcgggac gactcgaagg gagcgaatgt ccatgagccc caacacgacg attctggtcg
      481 tcgacgacga accggatgtg cgcgaagtgc tgcacgatta tttcgtcgcg cacggttatg
      541 cggtcgtcgg cgcgggcagc gccgaagcgg cgagggccga agctgcgcga cagccgatcg
      601 atctcgcgct cgtcgacatt cacatgccag gcgaggacgg actcgcactc gcccggcaca
      661 tgcgcgagcg ctacgcgaag atggcgatca tcatgctgac gtcggcgtcg acggtcgtcg
      721 accggatcgt cggattggaa atgggcgccg acgactacgt gccgaagccg ttcgatccgc
      781 gcgagctcgc cgcacgcgtg aagagcgtgc tgcgccggac ctcgtatgcg agtcgcgccg
      841 acatcggcgc ctcgcgcgtg cgcatcgggc gctgcgtgct cgacctcggt gcgcaccggc
      901 tgaccgacga cgaaggcacc gaggtcgcga tgtcgccgct cgaatacgac ctgctgaagg
      961 ccttcgccga gcatccgaac cgcgcgctgt cgcgcgagcg gatcctgaac ctcaacgatc
     1021 agcgtgactg ggacccgttc gatcgcagcg tcgaccttcg catcatgcgc ttgcgcaaga
     1081 aggtcgagcc cgacccggag catccgcgat tcatccggac gatacgcaac gaaggctaca
     1141 tcttcgtccc cgacggacga tgagggcacg atgaaccggc cgtggctcgc ccccccgcgc
     1201 atccggcgcg ccgtgcactt gctcgcgtgc ggcgcgcttg tgctgtcgct gttcggctgc
     1261 atgggacccc cggaaagcgc attgcgaatc ggcacgaacg tgtggatagg cagcgagccg
     1321 ctttatctcg cgcgcgacct tggccgcctc gattccgatc acgttcagct ggtcgaatac
     1381 ccgtcggcga gcgaagtgct gcgggcctat cgcaatcagg cgatcgacgg catggtgatc
     1441 agcctcgacg agctgttcgg actcgccgtc gacgggctgc agccgcgcat cgtgctcgtc
     1501 gtcgacgtgt cgaacggcgc cgacgtcgtc gtcggtcgtg ccgggatgcg ctcgatgcgc
     1561 gatctcaagg gcaaatcggt cgcggtggaa agcggcgcgc tcggcgcgtt cgtgctgagt
     1621 cgcgcgctcg cgctgaacgg catgcaggcg agcgatgtca atgtcgtgca tctcgagtcg
     1681 aacgagcatc cgggcgcgtt cgacaaaggg caggtcgacg gcgccgtcac gttcgatccg
     1741 taccgtgcgc agcttttggc gcagggtgct tcgacggtct tcgacagcac gcagattccc
     1801 ggcgagatcg tcgaccttct cgccgtgcgt gcctcggtgg tcgaccggca cccgaagacc
     1861 atccaggcgc tgctcgccgg ctggtttgcg gcgctcgact acatgaagcg cgagccggcc
     1921 gacgccgcgc agcggatggg catccgccag cagacgagcg gcgagcagtt cctcgaggcg
     1981 cagaagggcc tgcacattcc aacgcgcgag gagaacctgc gcatgctcgg cgggccgaag
     2041 cccgagctcg cgacggcggg ccgccgcctg atggccctca tgctcgatgc gaaattgctg
     2101 cacgcggacg tgaacatcga aagcgttctc gcgccgcagc cgctgctcga cctgccgcga
     2161 tgagcctgcg ggttcccgcg tcgctcaaga tcacggttcc gctgctcctg ctcgcgttcg
     2221 cggcgacgct cagtgcggtg aacctcgtct accacgtgcc gcaggccgag cgcggcgccg
     2281 aggaggagag ccgcacgcgg ctggcgcagg agatgtcgcg gttgcagagc acgctcgaat
     2341 acctgctgct gaagggcgac gcggaggccg cgcagcacga gatcgcggtg cttgcgcaca
     2401 atcacgacgt gctgctcgcc gcgctgaccg acgaacgcga catgatcatc gcggcgacgc
     2461 ggcgcgcctg gctcgggcgc cagatcgagg acgtgtcgcc ccagttcgac gccgatcagg
     2521 cggcgcgcgc aattcgcgag cgccgcgcgg gcatcgccat cgggctcaat ggcgatgtgc
     2581 tgctggggca cgcgggcatc ctgatgggca gcgagcgcga cgagcttcgt ccgtcgcgca
     2641 cgggaagcct gttcctcgcc tacgatctca agcgatacaa ggcggaggcg cgggcgcagg
     2701 tcgtgcagca atcgctctat tgggcgggat gggtgaccgc gctcgcgctg gccctgtggc
     2761 tcgtgtttca tttcctgctg acgaggcgca ccgcgcgtct cgtggatgcg gcggagcagc
     2821 tcgcggccgg caacctcggc gcgcggagcg accttggcgg acaggacgag ctcgggcggc
     2881 tcggtcgtgc gttcgacacg atggcgcgca aggtcgcggg gacgcagacg cggctgcgcg
     2941 aagacatcgc tgcacgcgag cgcgtgcagc gcgagctcga gatgtcggag gcgagctacc
     3001 gcgcgatctt cgacgcggcc gaggatgcga ttttcgtgca cgatatcgac accggtgcca
     3061 tcgtcgacgt caatccgaag gcgtgcgcga cgttcggcta cgagcgcgac gagttcaggc
     3121 gtctcgaaat cggcacgctg ggcaccggcg agcacccgta cgcgcagaag gacgcgatgg
     3181 cgttgattgc ccgcgcgcgt gccggcgagc aattgcgcgt cgagtggcgc gggaaggcca
     3241 agagcggcag cctgcgctgg tacgacgtct tctcgcgccg cgtcacgatc ggcggccacg
     3301 accggatcct ttcgcttgcc cgcgacatca ccgaccgcaa gcgcgccgag gccgcgcttc
     3361 gtgccagcga ggagcagtac cacgccatgt tcaacgcgtc gatcgacggc ctcgcgctgt
     3421 ggaacgcggc cggcgaagtc gtcgacatca atcccgcgct gtggcgcatg tacggccata
     3481 ccgacgccga attctcggca atgtccgcgc agcactggcc gcgtccttcg taccagcccg
     3541 agttcctgcg cgtggtcggc gcgggcgaat cgctgcattc ggaggtgacg gagtcgcgca
     3601 aggacggatc ggcgctgcag ctcgaggtgc acggcattcc gatgcagtat cagggacggc
     3661 cgcacgtgct gacgatcgcg cgcgacatca ccgggaagaa gcacgccgag gaggagctcg
     3721 cgcgccagcg cgagtcgctg taccagcgcg aaaagctcgc ggcattgggc tcgctgctcg
     3781 ccggcgttgc ccacgagctc aacaatccgc tgtcggtggt cgtcgcgcgc gcggtgctgc
     3841 tcgaggagca gggcgacgcc gcaacccagg ccgcggcgct caagatccgc acggcggccg
     3901 agcgttgcgc gcgaatcgtg cgcacgttcc ttgcgatggc gaggcaacaa cagcccgagc
     3961 gcgggccggt ggccatcaac gacgtcatca ctgccgcgct cgacatgacc ggttacgcga
     4021 tccgcacgag cggcatcgac gtttcgctcg cgctcgcgtc cgacctgccg ctcatcctcg
     4081 ccgatgccga ccagttgcac caggtgctgc tcaatctcat catcaacgcg caacagtcgt
     4141 tgcaggagca tccggcgccg cgccgcataa gcgtcacgag ctcgctcgac gcgggcggcg
     4201 agacgctgcg tatcgcggtt gccgacaacg gcccgggcat agcacctcag catcgcgcgc
     4261 ggatattcga tccctatttc acgacgaagc ccaccggcat gggcaccggc gtcggactcg
     4321 cggtaagcct cggcatcgtc gaatcgcatg gcggaacgct cggcgttgcc tgtccgaggg
     4381 aaggcggcgc catcttcacg gtcgcgctgc cggtgcgcca tgcggacgtg ggcgaagctc
     4441 cggccgcccc cgagcctgcg ccgagcccgc ggcggcgctc ggtgctcgtc gtcgacgacg
     4501 aagcggggat acgcgagtcg ctcgccgaga tcctgtgcgg tgcgcggcac cgcgtcgtca
     4561 cggcaagctc gggacgcgaa gcactggagc ggatggccgt cgagtactac gacgtcatcc
     4621 tcaccgacgt gcgcatgccc gatctcgacg ggcgtgcact ttatgcgcag atcgagcagc
     4681 gctggccccg ccaagccgcg cgcgtcgttt tcgtcaccgg cgatacgctc gcatccgagc
     4741 tgcgtgaatt cgtccgcaac agcggccgtc gcgtcatcga aaaaccattc ctgccttccg
     4801 aagtgcgacg gatcgtcgcg gagctggcgg cggacgccga gctttcgcga cctggctgac
     4861 gcgaaagtgc gttgtccgat ggacgtcgca gcgcggaaac aaacgaaaca atacggcgag
     4921 cgcgccgacg cattgctgca acggcccggc actagactgc actcattgca acgtgcaggg
     4981 aatggaccgt gagcttcgat gaatgctcga ccccgcgtgc cgcgctgggc ctcttgggaa
     5041 ttgccatggc ggcgatcacc atgggcgcgc tggtcgtgct gcccgcgaaa ctcgaggcgg
     5101 cgagcggtgg agtcacgggc gtgacggcgg ccacgacggc agcgggcgcc ggccccgcgg
     5161 ccgccggcaa cgcgcatgac gaacgattcg acgcccgcgt ctcgggcgat tcaaactgca
     5221 attcgacaag ggacagatca tgaactacga gggaagctgc ttctgcggtg ccgttggact
     5281 tgccgtgacg ggcgagccgg tggcggaagg ttattgtcat tgcgcgtcgt gccgcagctg
     5341 gtcggctgcg ccgctcaacg cattcacgct gtggaagccc gacgcggtca agttcacccg
     5401 cggcgaggac aacgtgggtg tctttcacaa gaccgaccgc agctaccgga aattctgcag
     5461 gacgtgcggc gggcacctgc tgaccgacca cccccagtgg gcgctcgtcg acgtgtacgc
     5521 ggcgaccatc ccggcgcatc gccatcgacc ggcattgcac gtgaactacg ccgagagcgt
     5581 gctccccatc agggacggcc tgccgaagat gaaagacctg ccgaaggaaa tgggcgggtc
     5641 gggcgaaacc tgcccggagt agcgcgacgc gatccggtcg ttccgatgcg cggccggcac
     5701 gggcgatgct gcgcccggtg acgcgatcag ccgcggtcga acggatgtcc gttcgacgcg
     5761 gcaatcatcg ccgcggcgtc gccgtacgtg cttcccggcg cgagtccgag aagcacgcag
     5821 gttacgccgg gcaaactgaa tgcgcagccg tgaacgttgc gcgcacttgc ctcgatggca
     5881 gcgcgatcgc gtccatcgag tgccttgagg aagagatcgc gcagcgccgg cgatggctgg
     5941 ccccaggtga cggcggggat gtgaaacatc gattctcctt ggcacgatgc gctgccggca
     6001 tcggatgatg tagcgcagcg gacgttgccg tgcggaaacg tcgggagcgt ccgcggcgtc
     6061 gcggacctcc ctgtctcttc tacgcggacg ggatgcctgt cgtcaagcaa tagtcgctca
     6121 aacggcatct tcgccattgc tcgcgagtcc ctgcaggctg ccgcgcagcc gctcctcgtc
     6181 gaggtcgaga tcgcgctcga cgcgccggcg gattgcgtcg ctcacttcgc cgctgcgcag
     6241 cagctcgttc agccgcttgc gttgtgcggc gacgagtgcg aggtcgagtc gatgcatgct
     6301 ggcggatgcg gcgaagcgct cgccgtttcc gtggcgttca ccctcgagct gggtgattcg
     6361 caactgctcg cgctcgcgcc accgcgcgat gacatcctcc ggcagcgcat acgtcctcgc
     6421 tgcggcgtcg agctcggcca gcgccgcgcg cgcagcctcg acacgcgcgt agatttcgcg
     6481 ctccttgcgc cggcgcagct ccgcccggcc gagcttgtcg agccgcaact tcctcaacac
     6541 ccacggtaac gtgagtccct gcgcgaccag cgtgacgacg atcacgacga acgtgagcac
     6601 gagaacgccg tcgcggcgcg gaaaggggac gtcgctggca atggcgatcg gcacgctgag
     6661 cgccgcggcg aggctcacga cgccgcgcac gccggtgaac gcgatgacga acggcatccg
     6721 ccagtttggc gccggatagc gcgcggcaat gcggggaaag agccagcgcg gcgcgtaggt
     6781 tgccggaaac acccagagaa acctgacgcc gattacaacc gcgctcacga gcaagccgtc
     6841 gatgacgaga tgggtcagtg tcgcggtgtc gaggccggcc gcgaccgtgc gcgcctgcaa
     6901 ccccgtaagc aggaagatcg tcccggtgat cacggtgttg gtgacgtccc agaagaacaa
     6961 cgcctgcagc cgcgtgttgg accggatcag cgccgcgccg gcgaagccca catacagacc
     7021 cgacacgacg gtcgcgagca cgcccgatcc gccgagcgcg tgcggcggcc agaacgcaac
     7081 gaacggcgtc agcagcgaca gcaggacctc gaggcgaggc tccgccgcga aatggcgaag
     7141 gcgcagaatc acatagccga cgacgaatcc ccacgccgtc tcggccacga gcaccaatgc
     7201 gaaatcgccc agggcttcgg ggagggaaaa gcgtcccgtg aaaaccgcgg tgagagccag
     7261 gttgaacagg atcagcgccg tggcgtcgtt gacgagtcct tcgccctcga gaatcgcgat
     7321 gatgcgccgc ggaatgctga gccgctcggc gaccgcaagc ggcgcaacca cgtccggcgg
     7381 cgacacgatc gcgccgagca tgaacgcgac gccgagcggg aacccgagca gccaatgggc
     7441 agcgcccgca accgcgacgg tcgtgacgat cacgcagccg aacgcgagca gcaggatggg
     7501 gcgcagattc tcgcgaaacg cctgccagct catcgtgaac gccgcgaagt agatgagcgg
     7561 gggcagcaac accagcagaa cgacatccgg gtgcagcgtg atcgacggca cccacggcat
     7621 cagcgcgagc gcgatgccgg cgaccaccag aaggatcggg ggaggcaggt gcaggcgcgc
     7681 cgcgatccag gcgacggcgc cgacgatggc aagcagcgcc gcgacggtgt acaggacttc
     7741 ggtcacgaga ctcgcgcgcg ttccgatccg gtcgtcagcg cgcggctggc gtcggcgcgc
     7801 gcactcggcg cgcggcggcg gacgcgcgag cgccgcgcac gatagcgcaa cgctttcggg
     7861 cagctacgaa gatctcttcg ccgacttctt cttcgctgcc gatttcttcg agggcgattt
     7921 cttcgccgcc gatttcttcg atgcggattt cttcgccggc gacttcctgg acgcggactt
     7981 cttcctgggt gacgatttct tcgcgggctt ttttttcgat gcggattgct tcgaagcgga
     8041 cttcttcgct gccgtcttct tcggtacctt cgcaccggcg cggcgcgctt cggacaggcc
     8101 gatcgcaatc gcctgcttcc ggctcttgac ctttctgccc gagccgccgc tgcgcagcgt
     8161 gccttccttg cgttcgtgca tgaccttcct gaccttcgcc tgcgcctttt ttccgtactt
     8221 cgccatgtcg ttcctccatt ggaccgggac gccgtgcgcc attttgcacc agatgcgata
     8281 ccgggtaacc tccggcgatc cacccgaaac gccaaaccca tgaacgccgc ccacgacgcc
     8341 ttcatcgccg cgcgcgattt cctgctcgcg caccgcaccg actacgacac cgcgatacgc
     8401 gatttccact ggccggcgct cacgcagttc aattgggcgc tcgatcactt cgacgtgatg
     8461 gcggccggga acgaccagcc cgcgctgtgg atcgtgggcg acgacgcgag cgagcggaag
     8521 cgatcgtttc gcgagatgtc cgagcgctcg tcgcaggtgg ccaatcatct gcgtgcgctc
     8581 ggtgtgcagc gaggtgatcg catcctgctg atgctcggca acgaagtcgc gctgtgggag
     8641 acgatgctgg cggcgatgaa gctcggcgcg gtcgtcattc ccgcaaccgc gctgctcact
     8701 gcagccgacc ttcgcgaccg catcaaccgc gggcgcgtgc gacacgtcgt cgcggcgcat
     8761 gcgcacacgc agaagttcgc ggaagtcgaa ggcgattaca cgcgcatctg cgtcggcacg
     8821 ccgtgcgagg gctgggtgag ccacgacgcg gccgacgcgt tcgcgcgcga gttcgcgccc
     8881 gacgcgccga cgctcgcaac cgacccgatg cttctctact tcacgtcggg cacgacgtcg
     8941 aaacccaagc tcgtgctgca cacccaccag agctatccgg tcggccattt gtcgacgatg
     9001 tactggatcg gattgcagcc cggggacgtc catctcaaca tctcgtcgcc cggctgggcc
     9061 aagcattcgt ggagctgcgt attcgcgccc tggaacgcgg gtgcgtgcgt gttcatctac
     9121 aacgccgcgc gcttcgacgc gcgcggcttg cttgcggcca tcgagcgctg caaggtgacg
     9181 acgctctgcg cgccgccgac cgtctggcgc ctcctgatcc agcaagacct cgccgcgtat
     9241 cgcggccgcc tcggcattcg cgagctcgcg ggagccggag aaccgctcaa cccggaaatc
     9301 atcgggcagg tgcgcgatgc gtgggggatc acgctgcgcg acggttacgg acagaccgag
     9361 accaccgcgc tgctcggcaa ctcgccgggc cagccgctga agcccgggtc gatgggccgg
     9421 ccgctccccg gctaccgcat cgcactgctc gacgtcgagg ggcgcgaggc agccgacggc
     9481 gaagtgtgca tcgatctcgg cgtccatcca ctcgggctga tgcgcggcta cgaggacagt
     9541 gaagaggcca cggcgcagtc gatgcgcaac ggttattacc acaccggcga caccgcggcg
     9601 cgcgacgcgg acgggtacgt gacgttcgtc ggacgcgccg acgacgtctt caagtcgtcg
     9661 gactaccgga tcagcccgtt cgagctcgaa agcacgctgc tcgagcatgc gtgcgtgacc
     9721 gaggttgccg tggtgccgag cccggacgcg ctgcgtctgg cgattccgaa ggcgtatgtc
     9781 acgctggccg cgggaagcga gccgagccgc gagctcgcgc gcgacatcct cgccttcgcg
     9841 cgccaggcgc tcgcacccta caagcgcgtg cgtcgcatcg agttcgtcgt ggaattgccg
     9901 aagacgctgt cgggcaagat ccgccgcgtc gagctgcgca aggccgaggc gttgcgccgt
     9961 gagcagggcg tgcgcgcgcc ggacgagtac ttcgaggaag attttcccga tctcaagcgc
    10021 ggatgacgcc gcctgcattg cgcaatccgc gcgagcctcc gtccggcgag cgagcaagcg
    10081 cattccggcg cgcggcttcc ccgcgacgtg gcacgatcgc agcgtgaata caacgacgat
    10141 ccgcgcgctg cacgcgcgcg aaattctcga ttcgcgcggc cggccgaccg tcgaggtcga
    10201 cgcgatgctg gacgacggca gcgtcggccg cgccagcgtg ccgtcggggg catcgaccgg
    10261 cgccgccgaa gcgcacgaat tgcgcgacgg cgaccccgcg cgttacggcg ggctcggcgt
    10321 gctgcgcgcc gtggccaacg tcaacacgga aatcgaccgc gcgctgcgcg gcgtcgacgg
    10381 cgccgaccag gcatcggtcg acgcgcgact gcgcgagctc gacggcacgc cgcggctgtg
    10441 ccggctcggc gccaacgcga cgctcggcgt atcgctcgcc gtgtgccgcg cgacggcggt
    10501 cgcgcaacgg caggcgctgt acgagcgcat cgccgagctc gcgcaaacgc aaccgctgct
    10561 gccgctgccg atgacgaaca tcctgagcgg cggctggcat gccggcggcg gcatggacgt
    10621 gcaggacttt ctcgtcgtgc cgatgcgcgc gacgtcgaca ggcgaggcga ttcacgcgat
    10681 tgcgcgcgtg cgcaccgcgg ccagtgacgt gttgcgcaag cgcggattgc ccacactcct
    10741 cgccgacgaa ggcggcctcg gtcccggttg ccggacgtcg cacgcggcgc tcgaattgat
    10801 gatcgagtcg atcgagcgtg cggaactcgc acccggaatc gacatggcga tcgccgtcga
    10861 tgtcgccgcg agcacgctgc tgcaggccga cggtcggtat cgtcttccgc gcgaaggccg
    10921 cgaatgcacg cgcgaggcga tgatcgacat gatcgacggc tgggtgcgcg aattcccgct
    10981 cgtgtcgatc gaagatccgc tggccgagga ggactggccg ggctggacgg cgttgacgcg
    11041 gcgcgtcggt ggtgtgctgc aactcgtcgg cgacgatctc ttcgcgacga attgcgcgcg
    11101 cgtcgcgcgc ggcatcggcg aaggcatcgc gaatgccgtg ctcgtcaagg cgaaccagaa
    11161 cgggacgctg agcggtacgc tcgacgtgat cgcgcaggcg aagcgcgcgg gctacgcgac
    11221 gatcgtctcg gcccgttccg gagaaacgga agatgcgttc atgtccgacc tcgcggttgg
    11281 cgccgcggcc ggccagatca agatcgggtc gctgcgcacg tcgagcacgg tcgccaagta
    11341 caacgagctg ctgcgcatcg ccgagcgctc cggggcgcgc tatgccggtg cggcagcgct
    11401 cgcgcccgct cgtcatcgat gacggtgcag cgcacgcccg acgcccttct gatcgagttc
    11461 gtgcgccgca ccgggctcgc cggcgacgcc gaaacgccgg tcttcacgcc gctcgaaggc
    11521 ggcgtctcat ccgatatctg gcgagtcgat ctggcgcagc ggtcgatctg catcaagcgc
    11581 gcattgccgc ggctcaaggt caggcaagcg tggacggcgc cgatcgagcg caaccgctac
    11641 gaatgggcct gggtcgagtt cgcggccgcg cactttcccg gttgcgtccc cgagccgatc
    11701 gcgagcgatc ccgttcacgg catcttcgcg atggcgtatc tcgatccggg ccgctacccg
    11761 ttgtggaagc ggcgcctcct cgatggcatc gcggagtccg gcgtcgcggc cgccgtcgcg
    11821 acgacggtcg cgcgcatcca ttcggcaagt gccggcgacg ccggcgtggc gcgccgcttc
    11881 gccaccgacg cgatcttcca tgcgatccgt ctcgagccgt atctgctcgc gaccgcggtg
    11941 cggcacccgc gtctcgcggc accgcttcgt gcgctcgcgc aacgcacggc gggcacgcgg
    12001 ctcgcgctcg tgcacggcga cgtcagtccg aagaacatcc ttctggggcc tcgggggccc
    12061 gtctttgtcg atgccgaatg cgcgtggtac ggcgatcctg ccttcgacgc ggcgttctgc
    12121 ctcaatcacc tgctgctgaa atgcatcgcc cgtcccgggt cgtgcaacga ttacctcgcg
    12181 tgcttcgaag ccttcgcgca cgcctatttc gcgggcgtga cgtgggaacc ccgcgcggcg
    12241 atcgaatcgc gcattgcagg cctgctcccc gcgctgttgc tggcgcgcat cgacggcaag
    12301 tcgccggtcg aatacatccg ggacgagcgt gaccggcagc gcgtgcgcga tgctgcatgt
    12361 ccgctgatcg aacggtcgcc gcagcatgtc gccgatgttg ccaccgcctg gcaccgtatg
    12421 atggctgcgc cgtgatgcgc ggccggtttg gagcgtgcac cgcttccggt atacgatccg
    12481 gtacgcctcg cacgctcgcg cgccggcgtt cgtgccggcg cctcgcgttc tcgcaattca
    12541 tcgaccgaaa tcaatcgctc ctatcgaagg gatgatcgct gaatgaagaa atcgtcaatc
    12601 gcagcgtggc tgacgggcac gcttctggcg gcggccgtgg ccgcgccgct cgccgcgcaa
    12661 ccgggcggca agctcgtcct ctacacgtcg cagcccgatc gcgacgcgca acgaaccgtc
    12721 gagggcttcc gcaagaagaa cccggctgtc gaggtcgaga ttttccgctc gggcaccacc
    12781 gaggtgatga gcaagctgat ggccgagatc gcggccggcg caccgcgcgc cgacgtgctg
    12841 ctgatcgccg atgcgctgtc gatggatcgg ttgaaggccg ccggcgagtt gcaggcctat
    12901 cccggggccg acgtgcgcga gttgccgccc gacgcctacg acaaggcaaa aacgtacttc
    12961 ggcacgaagc tcatcacgac cggcatcatc gtgaatcggc aggcgacgat gaagccgaca
    13021 tcgtggaagg acctgctgaa gcccgaagcg aagggacagg tggcgctgcc gagcccgctg
    13081 tattcgggcg cggccgcgat tcacatgggc gcgctccatg cgagtccggc gctcggcgcc
    13141 gacttctacg aaaagctcgc gaagaacggc gccgttgcac tgcgcggcaa cggcgccgtg
    13201 ctgaacgcgg ttgcgggcgg ccagaagatg tacggcatca tcgtcgagtt catggccctc
    13261 aatgccaggg ccaagggatc gccggtcgac ttcgtctttc ccgccgaggg cgtgtcgatc
    13321 gtgaccgagc ccacggcgat cctgcgcggg acgaaaaatc ccgaggccgc gcgcgccttc
    13381 gtcgatttcc tgctgtcgaa ggacggacag gagctcgcgg tgtcgcaggg ctacttcccc
    13441 gcgcggaagg acgtgaagcc gccgccggga tttcccgatg tcgcgtcgct tcggatcctg
    13501 ccggtcgaca tctccgaact cgagaagcgg gacgaactca acaagaaacg gtttgccgaa
    13561 ttgttcggcg gctgatcctc gcccacgatg ccgctgattc ctgcccggcg tggcgagcgg
    13621 ctgacgttcg caacgctgct cggcctcgtc gtcctgctcg ccgtggtccc gatcgcgcgc
    13681 ctcgcgcagg aagcgctggc gccgcatggc cggctcgacg cgggcgtgct cgcgagcgtc
    13741 gtcgaaagcc cggtcgtctg gcgcgcggcg cgcaacacgc tgagcgtgtc ggtcggtggc
    13801 gcagcgctcg ccatcgtgct cggtacggcg ttcgcgctgc tcgtgtcgct gaccgacgtg
    13861 cgcgcgaaaa agccgctcgt cttctgcttc atgatgccgc tcctgatcgc gccgcaggta
    13921 acggcactcg cgtggatcaa tctgctcggg ccgaacagca cgctgctgcg cgtgctcggg
    13981 atcgcgccgg ccgcgggcag cccgcatccg ctgtacgggc cgggcggcat cgtgctgctc
    14041 ctcggtctcg agcaggcgcc cgtggttttc ctcgccgtgc gtgccggcct gcgttcgctg
    14101 ccgcgcgacc tcgtcgaggc cgcgcaggcg gccggggcga gaccctggcg catcgtgcgc
    14161 acgatcgtgc tgccgctgat gatgcccagc gttgcggccg ggggcatgct cgccttcgtg
    14221 ggctcgatcg gcaacttcgg catcccggcc tttctcggca ttccggccgg gttcaccgtg
    14281 ctcgtcacgc tgatctacca gcggctcgcg ggcttcggca cgagcgtgct gtccgaggtt
    14341 gccgcgcttt cgctgctgct tgcgttgatc gcgctttgcg ggctcgcgct caatggctgg
    14401 cttgcgcgac gccgcgacgt gcgcgtgagc gcatgggcga ccgcgacacc gcaatggtcg
    14461 ctcggacgca tgcgcttcgc gatcgaggga acgtgctggt cggcgctatt cgcagtgctc
    14521 gcgctgccgt tctgcgcgct ggttgcaacg gcactggtgc cggccatcgg cgtgtcgctg
    14581 acgccgcaga cgctgacgtt cggcaatttc catttcgtgc tgttcgatta cgccgccacc
    14641 cgccgcgcgt tcgtgaacag cgcgtcgctc gccgccgcgg cggcggtgat gctggtgatc
    14701 gtgtcggtgc cgctcggtta ttcgctcgcc ggacgccgtg cgagcgtgcg ccaggccgtg
    14761 ggcttcgtcg ccgagctgcc gtacgcggtg cccggcgtcg tgctcgcaat cgcgatgatc
    14821 ctgtgcctgc tgcggccgct gccgctgctc ggcttgagcc tctacagcac gatctggatc
    14881 atcctcgtcg cgtatctgtc gcgcttcatg ctgcttgcgc tgcgtccggt caccaacgcg
    14941 ttgcggcaga tcgacccaag cctcgacgag gcggcccgga tgagcggcgc gcgctatgcg
    15001 cggcgggtgg ccaccgtcat catgccgatg atcgcgccgt cggcggtcgc gggtggcatg
    15061 ctggtgttcc tcatggcgtt caacgagttg accgtctcgg cgcttttgtg gtcatccggg
    15121 tcggagacgg tcggcgtggt gctgttcagc ctcgagcagg cgggcgacgc ggtgtcggct
    15181 gccgcggttg ccgtgatcgc cgtcgtcgtc acgctcggga tgatgctgat ggcgaacgcg
    15241 ctggcgtcgc gcgtcgcgcg cggagtcttg ccatggcagc cgtgacgctc gccggcatcg
    15301 gcaagcgctt cggctcgatt gcagcggtgg aggacgtgtc gctcggcgtc gaggaaggcg
    15361 agtttctcgc ggtgctcggt ccgtcgggtt gcggcaagag cacgctgctg cggctcatcg
    15421 ccggattcga gcgacccgag cgcggaacga tacggctggg cgatcgcctc gtcagcgggc
    15481 cggaggatta cgtcgcaccc gaagaccggc atgtcggcat ggtgttccag tcctacgcgc
    15541 tctggccgca catgagcgtc gccgagaaca tcggcttccc gttgcgcgtg cagaagcttt
    15601 ccggacgcga tctcgcgcgg caggtcggcg cggcgctcga cgcggtgggg ctgtcgggcc
    15661 tcggcgatcg gcggcccgcc gagctctccg gcgggcagcg gcagcgggtg gcgctcgcgc
    15721 gctgcctcgc gatgaaggcg cgcatcgtgc tgctcgacga gccgctcgcg aacctcgacg
    15781 tgcacctgcg ggcgcggatg ctcgccgaga tcgcggcatt tcgcacgcta tcgaaagcga
    15841 cgctcgtcta tgtcacgcac gaccaggccg aggcgctggg ggtggccgat cgcgtcgcgg
    15901 tcatggcggg cggacggttg ctgcaggtgg cgagtccccc ggcgctctat cgtacgccgc
    15961 aggacggcat cgtgagccgc ttcgtcggcc ccggcgcggt ggcgcgcggc gaagtcacgc
    16021 agacctacgc gaacgggcac gcgaaggtcg ccttgttcgg ggccgaatgg caggtgcgct
    16081 cggcaccggg ccagggcacg ggccctgcgg aaatctgcat ccgtcccgag gatctggccg
    16141 tcgtcgctcc ggggggcggg ttcgaggcaa gcgttgcccg cgtgctctac cagggcgggc
    16201 gcacgatcgt cgaggcggcc cctgcgagcg acccggcgac cctgctctcg ctctcgctcg
    16261 agtcgagcga gccgctgccg cccggcggga ccgtgcacct ggcactcgtc gacggttggg
    16321 tgatccccgg ccgatgaccc gcgcgaatct cgtgctgctg accggcgcaa cgggcttcgt
    16381 cggatcggcc atcgcgcgcg cgctcgtcgc cgccggctat ccggtgcgcg cgctggttcg
    16441 cgcgggggcc gtgcggaaca acctcgccgg tgtgcctctc gaaacgttcg atggcgacat
    16501 ctgcgacgaa gcggcgctcg cgcgggcgat gtccggcgtt cgttacgtcg ttcacgccgc
    16561 cgccgattac cggctgtggg ctcgcgatcc atcggcactc gtgcgcacga atgtcgatgg
    16621 cacgcggctc gtgatgcaag ccgcgctgcg aagcggggcc gagcgtgtcg tgtacacgag
    16681 cagcgtggcg acgctcgcga cgcgggccac gggcgatgcc gccgacgaga ccgcgccgct
    16741 cgacgagcgc gcggcgatcg gcgcgtacaa gcgcagcaag gtcgccgccg aacgtgtcgt
    16801 cgaggcgatg gtcgcgcgcg acgggcttcc agcggtcatc gtcaacccgt caacaccggt
    16861 cgggccgcgc gacatcaagc cgacgccgac cggacgcatc atcgtcgagg ccgcgcgggg
    16921 acgcgtgccg gcgtatgtcg acacgggaat gaacctcgtc cacgtcgacg atgtcgcgga
    16981 cggccatgtt gcagcgctca cccgcggccg ggtcggcgag cgttacattc tcggtggcga
    17041 caacgtctcg ctcgtgacca tgctctcgga catcgcgcaa ctcgccggcg gccgcgctcc
    17101 ccgatggaag ctgccgcgcg gcccgcttta cccgctcgcg gctgtcgcgg aggcgtacgc
    17161 gcgggcgacg tcgcgcgagc cgtttctgac gcgcgacggg ctgcgcatgt ccgagcaccg
    17221 catgttcttc acgagcgcca aggcgtcgcg cgagctcggc tttcgcgcgc gtccttatgc
    17281 cgagggcctg cgcgacgcgg tcggctggtt tcgcgcggcg gggtatctgc gatgagcgtg
    17341 gccattgccg cgctcggctg tgtcgcctgg ctctacctgc tcgccgcgcg cggcgggttc
    17401 tggcgtgccg gcgaccgcga cgatgcgccc gccgccattg ccgatctcga cgcgtggccg
    17461 cgcgtgaccg ccgtcgttcc ggcgcgcgac gaagccgacg tgatcggggc gaatctcgcg
    17521 tcgctgctgc ggcaggatta tcccggcgcg ttctcggtga tcgtcgtcga cgaccacagc
    17581 agcgatggca ccgccgctgt cgcgcgcgcc gcggcgggtg acggaacagc atcacgcgtg
    17641 acggttcttc ccgcacccgc cgtcgagccg ggctggacgg gaaaactctg ggcgctgcgt
    17701 cacggcatcc atcattccga ggccttgccc gcatcgtccg actacctgct gctgaccgac
    17761 gccgatatcg cgcacgcacc cgacacgctg cgcatgctgg tttcgcgtgc acggcgcgaa
    17821 cgcctgctgc tgtcgtcgct gatggcgaag ctcgatgtgc aaagcgccgc cgagcgggcg
    17881 ctgattcccg cgttcgtgtt cttcttccgg atgctctatc cgtttgcatg ggtgaacgag
    17941 ccgcggtgcg cgaccgcggc cgcggcgggc ggctgcatgc tcgtcgatcg caatgcgctg
    18001 cgtgcggccg gcggtctcgc ggccattcgc ggcgaactga tcgacgactg cgcgctcgcg
    18061 cggctcctca agccccacgg tccgattcgc atcgcactta ccgatcgcgt gcgcagcacg
    18121 cgcgcgtatc ggtcggtcgg cgagctgcgg cgcatgatcg cgcgcaccgc gttcacgcag
    18181 cttcggttct cggtgtggcg tcttctggcg acggtcttcg cgctgctcgt cgtgttcgtg
    18241 gcgcccccgc tgctggcgct gccgtccgcc gacccgctcg tgcgcacgct cggcggcctc
    18301 ggctgggcct gcatgacgct cgcgttcgtg ccgatgctgc gcttctaccg gcgctcaccc
    18361 gcatgggcgc tcgcactgcc cgcgatcgcc gcagcctatc tcgccttcac gatcgattcc
    18421 gctttccagc atctgcgcgg ccgcggcggc gaatggaaag gacgcgtgcg tccgcgcgcg
    18481 gcgttaaccc ctgcgagccg gcgatgacga cgacgcgcgc cgttaaccgc ttgcgctgca
    18541 ggcgccgctg atgcgcaatc ccgagcacat cgcgcggcga aggcgtggcg acgagcgcga
    18601 cacgcgcgtc gacgcggcga tcgacgaggc gacggcggca ttgctcgacg cgcagcgtgc
    18661 cgatgggcac tgggtgttcg agctcgaggc cgactcgacg atctcgtccg aatacatcac
    18721 gctgatgcac ttcctcggcg agcgcgatgc gccactcgaa gcgcgaatag cgcgctatct
    18781 gcgccggcgg caggcggcgc acggcggctg gccgctcacg cacggtggcc cgttcaacat
    18841 gagcgcgagc gtcaaggcgt attacgcgct caagctcgcc ggcgattcag cggatgccga
    18901 gcacatgcgg cgggcgcgcg atgcgattct cgcgcatggc ggcgcgggca ccgcgaacgt
    18961 gttcacgcgc atcctgctcg cgctgcacgg catcctcacg tggtcgtcgg tgccggtggt
    19021 gccggtcgag atcatgctgc tgccgcgctg gttcccgttt catctgtcgc gcgtgtcgta
    19081 ctggacgcgg acggtgctcg tgccgctgct cgtgctgcag gcaaaaaagc cgcgcgcacg
    19141 caatccccgc ggcgtgcgca tcgacgagct cttcccgaac ggccggccgc atgcgcgacg
    19201 ctgggcacgg gcgccgcatc aggcgcgcat cccctacgcg gtgtttgcgg cactcgacgt
    19261 gctgctccac ggcatcgaac ggctgatccc gcgcggcctg cgccagcgcg cgatcgagcg
    19321 cgcgctcgcg ttcgttgccg agcgcgccaa cggcgaggac ggtctcggcg cgatctttcc
    19381 ggcgatggcg aacgccgtga tgatgtacga cacgctcggc tatgcgcgcg accgcgagcc
    19441 gtacgccacc gcgcgcaagg cggtcgacgg tttgctcgtg gtcagcggcg acgaagcgta
    19501 ttgccagccg tgcgtttcgc cggtgtggga taccgcactc gcatgccatg cgctgctcga
    19561 cgcgggcgat cctgcggcga tcgcgtcggt gcatcgcgcg ctttcatggc tcgccgagcg
    19621 acaggtgctg acggtggccg gcgactggaa ggactgccgc cccggcgtgc gtccgggcgg
    19681 ctgggcgttc cagtatgcga acgcgcatta tcccgatctc gacgacacgg cggtggtcgt
    19741 gatggcgatg gatcggtggc tgcatcttca cggtgtgcac cccgagccgc gtctgcgcga
    19801 gacgatccgg cgggcacacg agtgggtgct cggcatgcag agcagcaacg gcggatgggg
    19861 cgcattcgac gccgacaacg tcagcgaata cctcaaccac attccgttcg ccgatcacgg
    19921 cgcgctgctc gacccgccga ccgccgacgt caccgcgcgc tgcgccggca tgctcgcgca
    19981 gcttggcggc gcgaccgatg cgcccgccat cgccagcgcg ctcgcgttcc tgcggcgcac
    20041 acaggagccc gacggctcgt ggtacggccg ctggggcatg aactacgtgt acggcacgtg
    20101 gtcggtgctg tgcgcattcc acgctgccgg cgtttcgccg cagtcgctcg acgttcgccg
    20161 cgccgtcgac tggctgtcga gcgtgcagaa cgcggatggt ggctggggcg agacggcgca
    20221 gacgtacgag ctcggttatc gcggcgacaa gcgcggcccg tccaccgcgt cgcagacgtc
    20281 gtgggcgctg cttgggctga tggccggcgg cgagacgcgc aatcaggccg tggcgcgcgg
    20341 cgtcgattat ctgctgcgca cgcagggtga cgacggattc tggcccgagc cggagttcac
    20401 cgccaccggg ttcccgcgcg tcttctttct tcgctaccac ggctatgcga agttcttccc
    20461 ggtctgggcg cttgcgcgct accgcgcgca gctgtcctcg tcgctgccga ggatgcggct
    20521 cgggatgtga gctcgccggc tgcgcagcca gcccatgccg ggttcgttct ggtcgcgtgc
    20581 gggctcgcgc tggaagcgcg cattgccagc ggaccgggcg tgcgaagcat cgccggcggc
    20641 gtcgaccggc agcggctcgc ccgcgagctc gaggatgcag tgacagcggg cgcggaagcg
    20701 gtcatcagct ttggcatcgc cggtggactc gcccccggtc tgcgtgcggg aacgcggatc
    20761 atcggccgcg gcgtgcatgc cgccgccggg taccgcgcgt gcgacgccgc atggattgct
    20821 cggcttgccg agcgcctccc cggcgcgctc gtcggcgatc tcgcgggcgc cgatcgaccg
    20881 gtcgcgaatg ccgacgcgaa gcgcgcgctg catgcggcga catccgcgct cgcggtcgac
    20941 accgaatcgc acgttgctgc cgacgttgcc gtcacgcacg gattgccgtt cgcggcattg
    21001 cgcgtggttg ccgacgcggc gtttcgcagc ctgcccgagg ctgcgctggt gggactcgat
    21061 gcaggcggca cggtcgatgt cgcggcggtg ctgcgctcgc tcgcgcggac gccgtcgcag
    21121 ctgccactgc ttctgcgcac cgcttgcgat gcgcgcgttg ctttcgcggc gctacggcgc
    21181 gggcgccgtt gcgttggcgt gcggttcgga tgcgatcttc gcgcgctcga tctcgacgtg
    21241 acgtgagaag acgtactgcg cggggcgttg ctggccgagc gggatctcgg gcgccatcgg
    21301 gccgtgcgtg cgcacgccgc gcacgctgac cgcgagcgcc ttcagcggat ggcgcaccgc
    21361 gtcggtgacg gcggtggcct cgtagccgct gtgcaccatg caattggcgc acttctcgta
    21421 gttgccggtg ccgtacgcgt cccagtcggt ctcgtccatc aattcgcgaa acgactttgc
    21481 atagccttcg cccagcagat agcaaggccg ctgccagccg aagacgttgc gcgtcggatt
    21541 gccccacggc gtgcaccggt attcctcgtt gccggcgagg aaatcgagaa acaggctcga
    21601 ctggctgaac gaccacgcac gtccgccgcg gccgcggctc aggatgtcgc ggaagagctg
    21661 cttcgtgcgt gtgcgcgtca ggaagtgcgc ctggtcgggc gcccgctcgt acgcgtaccc
    21721 cggcgaaacg gtgatgccgt cgacgccgag cgcggccacg ctgtcgaaga acgcggccac
    21781 gcgttcgggc tcggcatcgt tgaaaagcgt gcagttgatg ttgacgcgaa agccgcgcga
    21841 cttcgcctcg ccgatcgcgg caacggcgcg atcgtagacg cccgattcgc ataccgactt
    21901 gtcgtgcatc gaccggtcgc cgtccagatg caccgaccat acgaaatacg ggctcggcgt
    21961 gaacttcgcg aggcgctttt tcatcagcag cgcgttcgtg cacagataga cgaacctgcg
    22021 ccgctcgacg atgccgcgaa cgacgtcgac gatttccttg tgcagcagcg gctcgccgcc
    22081 ggcgatcgag acgaccgggg cgccgcattc gtcgacggct tcgaggcatt cggccaccga
    22141 caggcgccga tcgaggattt cgcccgggta gtcgatcttg ccgcagccgg cgcacgcgag
    22201 gttgcagcgg aagagcggct cgagcatcag caccagcgga tagcgccgtc gtccggcgag
    22261 ctgctggcgc atgacgtacg caccgatgcg tgttttctgc agaagcggga tggccattgt
    22321 ttttagtgtt ccgtcaggtt gcgccgtgcc cggcgtgctt gcgcagcaat tcggccggca
    22381 ggcggaactc gaccttctcc tcgatgccgg ggagcgtcga cacctcgatc ggcccgagtg
    22441 cgcgcaacgc gtcgatgacg ccgtcgacga gcgtctcggg cgccgatgcg cccgccgtca
    22501 cgcccaccac ctccgctgct ttcacccacg ccgggtcgag ctcggtgccg tcggcgatca
    22561 ggtagctcgg cacccccgat tcgacgccga tctcgcgcag ccggttcgaa ttggagctgt
    22621 tgcgcgcgcc gacgacgagg atcacgtcgg ccacccgcgc gagctcgcgt accgcggtct
    22681 gccggttttg cgtggcgtag cagatgtcgt ggatgtcggg gccgacaatg ttcgtgaagc
    22741 gtctggcaag cgccgcgatg acgctgcgcg tgtcgtcgac ggagagtgtc gtctgtgtca
    22801 catatgcgac aggcgtatcg ggcgcgagtt cgagccgctc gacgtcctgc tcgttctgca
    22861 cgagcagcac gggccccggg atctggccca tcgtgccttc cacctcggga tgtccggcat
    22921 ggccgatcag gatgaccgta cgtcccttcg acacgtagcg cttgccctgg ccgtgtacct
    22981 tcgccacgag cggacaggtg gcgtcgagca cgcgcaggcc gcgcgtgcgt gcatcgtcct
    23041 cgacgacgcg agccacgccg tgcgcgctga acacggtgat cgatccgacc ggaacctcgg
    23101 acagatgctc gacgaaacgc gcgcccttgg cgcgcaggct ttcgacgaca tgccggttgt
    23161 gcacgatctc gtggcgcacg tagacgggcg cgccaaagcg ctcgagcgcc tgctcgacga
    23221 tgtcgatcgc gcgtatgaca cccgcgcaaa agccgcgcgg ctgcgcaagc atcacccgca
    23281 tggcagaggt cgcgtcgacg gggaggatgg aacgagggcg tacatttgct actcccgatg
    23341 atccgctatt gcgcgccgcg agacaaggcg gcgcgcgaaa cgatggcgat atgccgatga
    23401 ccgatccaac gccggaacac cggcgcaggg atgcccgcgg gcccgaccgc ggactggttt
    23461 cgccgatccg caagacggtg atcgcgtgcg cggcgcaccc gttgccggtg atcgtgacgg
    23521 ccttcctgct gtgcgcggcc gcgctcgttc acgtgatgac gcattttgca atcgacaccg
    23581 acgcgtcgaa gctcatttcc gccgacgtgc cgtggcgcca gcgggagctc gccttcgccg
    23641 ccgccttccc gcagcgcgtc gacctcatcg ccgtcgttgt cgacggtacc acgcccgagc
    23701 aggcagacac ggcgacggcg cgacttgccg agcgcctggc cggcgagcgc gcattgttcc
    23761 gcgccgtgtg gcgtccggac ggcggtgcgt tcttcgagcg cgagggactc ctgttcgaat
    23821 cgacgcagga gatcgcgcag acgacgcagc gcctgattgc cgcacagccg ttgcttggca
    23881 cgcttgccgc cgatccgagc cttcgcgggc tgatggacgc gctgtcgctg gtcgcgatgg
    23941 gcgccgcgcg ggacgcggcc gcgctcgatg cgatgggcaa gccgctcgca gcactcgcca
    24001 cggcgctgga ggcgatcgcc gccggccagg ccccggcgtt ttcatggcat gcgctcttca
    24061 ccggcgaggc gcccgatccg cggctgctgc gccgcttcat cctcgtgcag cccgtgctcg
    24121 attacgcggc gctgcagccg ggagagcgcg cgacggcggc gatccgcacc gcggcgcggc
    24181 agctccatct cgacgccgtt gaaggcgtgc gcgtgcgatt gacgggcccc gtgccgctcg
    24241 ccgacgagga attctcgacg ctcgccgagg atgcgggact caacgccacc gtgacgctcg
    24301 gcgtggtcgt cgcgctgctc tggatcgcgc tgcgctcgtg gcggctgatc gtcgcgatac
    24361 tcgtgagcct tgcgatcgga ttgatcgcca ccgcatcgtt cggcctgctc gcgtttcgcg
    24421 cgttcaacct gatctcggtg gcgttcgcca tcctgttcgt cgggctcggc gtcgatttcg
    24481 gcatccagtt ctcgatggcc tatcgcgcgc gcctgcatgc aaccggcgat tcgcgtcatg
    24541 cgctgcgcga catcggcggc gaggttggcg cagcgctcgc gctcgccgcc gcggcgacgg
    24601 cggcgggctt ctttgcgttc gtgcccaccg attatcgcgg cgtgtcggag ctcggcgtga
    24661 tcgccggcac cggcatgatc gtcgcgttcg cggcgagcat caccgtgctg cccgcgctcg
    24721 tcgcattcct gagaccgcgc gcagagcgcg ccgaagtcgg ctatccgggg cttgcaccgc
    24781 tcgatcgctg gctcgtcgtg catcggcgca tcgtgctcgc ggcgggagcc gctgtcgcgc
    24841 tcgccggcct cgcactcgtg ccgaagctcg tattcgactt caacccgatg catttgcgca
    24901 gtcccgaggc cgagtcggtg gcaacgttgc tcgacctcat gaaggactcg gccaccgcac
    24961 ccgacacgat cgatgtgctt gctccgtcgc tcgatgcggc cgtcgggctc ggcgagcggc
    25021 tcgcgcaact gccggaggtc gatcacgtac tttcgctcgc gagcttcgtt cccgaagcgc
    25081 aggatgaaaa gctcgcgctg atccaggatg cggcgttgct gctcgagccg acgctcgcac
    25141 cggattcaat caaggcggca ccgaccgatg cggccgccgt cgccgcaatg tcgcacgcgg
    25201 cgcagcgact cgacgaggcg cgcgcgggcc gctcgggaat gccgttcgcg acgaccgccg
    25261 cccgcctggc cggcgcattg cgtgcgttgg ccgacggtcc gcctgcggtg cgcgagcgtg
    25321 cgcgcacgct gctgatcccc gggctgcaaa cggcgctcgc gcagcttcgc aacgcgctgc
    25381 aggcggcgcc cgtcacgctc gcatcgctgc ccggcgcgct gcgtcatgac tggataagcg
    25441 ccgacgggcg ggcgcgtgtc gaggtgtatc cgaagggcga tgtcagcgaa aatgccgcac
    25501 tgcgccgctt cgtcgacgcg gtgcaggcgg tcgcgcccgg cgccaccggc gcaccggtat
    25561 cgatcgatgc gtcgagccgc acgatcattg gcgcgttcgt gaaggcaggc gtgctggcct
    25621 tcgtgtcgat cacgctgctc ctcgtgctga cgctgcgccg cgcgcaggac gtcgtgcgca
    25681 cgctgctgcc gctgctgctg tcggcgctcg gcacgctcgg cagctgcgtg atcatcggcc
    25741 tgccgatgaa cttcgagaac atcatcgcgc tgccgctgct gttcggcatc ggcgtcgcct
    25801 tcaacatcta cttcgtgatg gcgtggcgcg cggggcggcc gaacccgctg caatcgagtc
    25861 tcacgcgcgc ggtggtcatg agcgggctca cgacgggtac cgcattcgga agcctctggc
    25921 tgtcgcatca cccggggacc gcaagcatgg gcgagctcct cgcgttgtcg ctcgcgtgga
    25981 cgctcgtcgc ggcgctgctg ttcctgccgg cgctgctcgg cgagccgcgc cagcgaccgt
    26041 gagtcgggtc agacgaattg cttgcacgtc acgcgtgtcg acaagccgtc gccgaccgct
    26101 cggcgcgatg ccaggcgaaa agaccgggga cgtagaagac gacgtcgcgc aaacggcgcg
    26161 ccgcggcgag tgcgagcgcc gtcgtcgcgt cgagcccgag cacgccgccg atcagcacga
    26221 atgcgccttc ctggacgccg atcgcggcgg gcaccacgaa cgcgacgctg ttgatcgcct
    26281 gcacggtcgc ctcgatggcg atcgcctcgt gaacgctgcg cggtgcgccg aggaaatgga
    26341 gcgcgagcca ggtctcgccc gcgctcacga cccacgcggc gaactgcagc acgacgcaga
    26401 cgacgacgtc gcgatggcgc gcatgcacgt cgcgcagcgc ccggtcgatg cgcagcgacc
    26461 gcgccctcgc cgagcgcagc cggcccgaga agagccgctc ggcgacgccg gcaaggctcg
    26521 cgagggcgcc cacgcgctgt gcgagcagga gcgcgccgcc agcggcaagc agggccgcgg
    26581 cgccgaacgc aagctcgcgc gtgaacgcgc cgccgtgccc cgcgatcacg agcatcgcaa
    26641 ggcccagcag cgcgaacgcc gactggctca gaaccgataa cgcgacgtcg gcggcgaggc
    26701 tcgctgcaac ggccgcgcgc gatgcgacgt ggcgccgcag cacccggtac gcggcgatct
    26761 cgccacccac gcgtgcgacg ggcagcacgc cgttcaccga ctcgcgaatc cacaccgcgt
    26821 tgagcagaac ccggaggcgc ggacgcatcg gctgcggaaa cagccactgc cacgcgagcg
    26881 cgttcgtgac catcggcacg acgtgaaacg acgccgcgag cagaagcccc gggatcgcgc
    26941 cggcgacgag cagcgcgatg cgcgtcatgt tctcgtgggc gaaaagcccg gccgcgagcg
    27001 caaggccggc aatggcgagc acgcgcgctg cgcgcttcat gccgcgatga aatccgagta
    27061 accgccggtt gcgatgtcgc tgccgcgaag cagcttcacg acactcggcg caacgagtgc
    27121 ggcgagctcc tccgcgtggc cgccgctcgc gccggcgaag ccgtcgcttg tcgccgggtg
    27181 cgcgtagatc tcgctcacgc cgtcgggaag gtggtgcagc aggccttcga gccgcggccg
    27241 cgtcatcgcc cccgaccatg cgaggccgaa cacgttcgcc ggcgtcatca cgccgcgccg
    27301 gcgcgtgcgc cgggcgagga ggcgggtgca gagtttcgcg agccagtcgc cgtgcgggtg
    27361 ctgcgaaggc tcgatgcgcg caaggacgcc ggccggctcg cgcggcacgc gcaggccgcg
    27421 cacgccgcgc gtcgcgccga tctcgagcac gagtgctgcg accgtcgggt gcagatggaa
    27481 gtgatggtgc gcgttgacgt gatcgagcgc gagcccggtt gcgcggaacg cgtcgaattg
    27541 cgccgcaatc tccgcgcgga gctgccggcg tgcggacggg ctaaagaaga tgttgcgccc
    27601 ggcgcgcgcg agatcggccg ggaagcggcc gtcggcgccg acgagatcgc acaccgcgtg
    27661 acgcggcagc accggagcgc cgtcgacgag caccaggtgc agcccgacgc gcagcgccgg
    27721 cagccggcgg gcgcgcgcaa cggcatcgtc gacggccgcg ccgctcatca tcaggctcgc
    27781 cgccgtgagg atgccgcgcg tgtgggcaag ctcgacggcc tcgttcacct cgcgcgccgc
    27841 gccgaaatcg tcggccgtga cgatgagctt ccttgggaat ccctgaacga ttcccacgcc
    27901 ggcgttccta ctgcgcggtg ccgcggcgct cgcgcaggaa ctggaagaac tcgacgcctt
    27961 cgcgcagccg ccgcttcatc atctgcgggc ttttcaccat ttcgccgacg atcgacgcga
    28021 tcttgccgct tcgaaagtag aagcgccggt agaacgtttc gaccgaatcg aagatctcgg
    28081 tatgcgaaag gtgcggatag tggagcggtg cgatctgcac gccgtgcgcg tcgacgagct
    28141 ccgcgtgcgc gtcgtcgagc cagccgttct cgagcgcttg ccggtaaaga tacgtgccgg
    28201 ggtagggggc cgcgagcgag acctggatcg tgtgcggatt gatctcggtg gcgaagcgga
    28261 tcgtctcctc gatcgtctcc ttcgtctcgc cgggcaggcc caggatgaac gtgccgtgga
    28321 tcgtgatgcc gagatcgtgg cagtccttcg tgaagcggcg cgcgaactcg atgcgcatcc
    28381 ccttcttgat gttgaacagg atctgctggt tgccgctttc gtagccgacg agcagcagcc
    28441 gcagtccgtt gtcgcgcatc accttcaacg tctcgcgcgg cacgttcgcc ttcgcgttgc
    28501 acgaccacgt gatgccgagc ttgccgagct ccttcgcgat cgcctccgtg cgcggcaggt
    28561 cgtcggtgaa cgtgtcgtcg tcgaagaaga attccttcac ctgcggcagc gcctgcttcg
    28621 cccacgcgat ctcctcgatc acgtgaccga cgctgcgcgt gcgatagcgg tggccgccga
    28681 ccgtttgcgg ccacaggcag aacgtgcagc gcgacttgca gccgcggccg gtgtagagcg
    28741 acacgtacgg atgcttgagg tagccgatga agtagcgctc gatttcgagg tcgcgcttgt
    28801 acaccggggt cacgaacggc agcgcgtcca tgttggcgag cggcgcgcgc ggcgggttgt
    28861 ggacgatgcg gcccgcttgg ttgcgccacg aaagcccggc gatggtatcg agatcgcggc
    28921 cctcggccac ctcgcgaatc gtgaagtcga attcgttgcc cgcgacgaag tcgatggcgg
    28981 cggacgccgc gaggcttccc tccgggtcca ccgcgacctt cgcgccgatc agcccggcct
    29041 tgagatcgcg attgactcct ttcattgcct cgatcgtctt gacgtcggag gcgaacgacg
    29101 gtgccgaggt atgcagcacg gcgaggtcga agtcgcgcgc ggtggcgaga acgtcggaca
    29161 gcgcgaggcc gtgcggcggc gcgtcgacga gcttgctgcc ttcgacgagt gcggcgggct
    29221 gcgcgagcca cgtcggatac cagaacgagc ggatctcgcg ccgcgcctga tagcgcgacc
    29281 ctgcgccgcc gtcgaagccc tcaaatgacg gcggatgcaa aaacagggtc ttcatgcggc
    29341 actgtctcct tcgagggtga gttgtccttg gcgatccaca tggaacgtgc ggttgcgcc
//
LOCUS       DASSVX010000008         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_233509, whole genome shotgun sequence.
ACCESSION   DASSVX010000008 DASSVX010000000
VERSION     DASSVX010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233509"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2091
                     /locus_tag="VFO53_00500"
     CDS             <1..2091
                     /locus_tag="VFO53_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET8874780.1"
                     /translation="LFVKAILASEDKNFFTHDGIDKGAIVRALGKRVLQESRSGASTL
                     TMQIAKHLRGGTGRPSTEIEKIGDIVMALRIERAYSRNELLRSYVNMPYFGRGQYGIE
                     AASRAYFGKSASALALHQAAFIVSLINKPALPDRAFATDPMLRTPEKIHDANWAEAVR
                     GTIRVLDLMRDQHVISDVEYDRAANLVEKSLRKEVVAPGMACGTRDHFLERVRLLYKD
                     RFPINRGGLTISITRDDALQEVLENAVDLTLRTYLARHQGDTDNDELRAGAFAVDFTG
                     DVLAEVGNVDFRKFKYDVIATGWRQPGSTFKIFTYGGLVERLTQEVLATAPASESPDD
                     IAAQILERCTVQDAPVFVPLGRGRGVKKIENFHSRSEPEYRGDISCRIALGESRNTAA
                     MRAGARAGIRNVIDLAYRLGMPRDPRHMLQPYPTTAIGASEVNPLAMATTAAFVNGGF
                     RVTPRFANDVCHDGRSLLYDDDTGKPKACDIKGEHHATQERLMHPAVSNAMIELLKGP
                     LDIGPTGTAAGLRTGVIPGMDPLSDAVWKLTPQQRKKRTLSFPLDLAGEIAGKTGTAT
                     NADGKTSDVWLMLFVPGPPEHPEKGVVLGFWMGKDSKDHPLGSRGATGGPGFAESGAR
                     NWTHSAATVLAFLQKERGLLRPGYRFRPIIRDDVQQQVDAKTAEPDAIVPVAAVGPLV
                     AASTAKPAPDPARP"
     gene            complement(2153..2953)
                     /locus_tag="VFO53_00505"
     CDS             complement(2153..2953)
                     /locus_tag="VFO53_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004864474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET8874781.1"
                     /translation="MPEIIAGLARHGIRGIAPWRDQVAKAGLKATAQQIRDAGLTVSG
                     LCRGGMFPAPDRQARRAALDDNRRAVDEAAALGAQCLVLVVGGLPKDARGAIVSNDIA
                     GAREMVRDGIGELLDYARGAGMPLAIEPLHPMYAADRACVNTMAHANALCDELSPNGK
                     DGLGIAVDVYHVWWDPELRAEIERAGAHDRLYAYHICDWLVPTADLLNDRGMMGDGVI
                     DLRLIRSWMEAAGYRGMHEVEIFSANNWWKRNGDEVLLTCRTRHEQSS"
BASE COUNT          503 a         1012 c          989 g          455 t
ORIGIN      
        1 cttttcgtca aagcgatcct cgcttccgaa gacaagaatt tcttcacgca tgatggcatc
       61 gacaagggcg cgatcgtgcg ggcgctgggg aaaagggttc tgcaggaaag tcgttcgggt
      121 gcgtcgacgc tgacgatgca gatcgccaag cacctgcgcg ggggcaccgg acggccgtcg
      181 accgagatcg agaagatcgg cgacatcgtg atggcgctgc gcatcgagcg cgcgtactcg
      241 cgcaacgagc ttttgcgaag ctacgtgaac atgccgtact tcgggcgcgg gcagtacggg
      301 atcgaggcgg cgagccgcgc ttacttcggc aagtcggcga gcgcgctcgc gctgcaccag
      361 gcggcgttca tcgtgtcact gatcaacaag cccgcgctgc ccgaccgggc gttcgcgacg
      421 gacccgatgc tccggacccc cgagaagatt cacgatgcca actgggcgga agccgtgcgc
      481 ggcacgatcc gcgtgctcga cctgatgcgc gaccagcacg tgatctcgga tgtcgagtac
      541 gaccgcgccg ccaacctcgt cgagaaatcg ctgcgcaagg aggtcgttgc gcccggcatg
      601 gcgtgcggca cgcgcgacca cttcctcgag cgcgtgcggc tcctgtacaa ggatcgcttt
      661 ccgatcaacc gcggcggcct cacgatatcg atcacgcgcg acgatgcgtt gcaggaggtg
      721 ctcgagaacg ccgtcgatct cacgctgcgc acctacctcg cccggcatca gggggatacc
      781 gacaacgacg agttgcgcgc gggcgccttc gccgtcgact ttaccggcga cgtcctcgcg
      841 gaggtcggca acgtcgactt ccgcaaattc aagtacgacg tcatcgcgac cggctggcgc
      901 cagcccggat cgacgttcaa gatcttcacc tacggcggcc tcgtcgagcg gctgacgcag
      961 gaagttctcg cgacggcgcc ggcgtccgag tcacccgacg acattgctgc acagatcctc
     1021 gagcgctgca cggtgcagga tgcgcccgtg ttcgttccgc tcggccgcgg acgcggcgtg
     1081 aagaagatcg agaatttcca ctcgcgctcg gagcccgagt accgcggcga catcagttgc
     1141 cggatcgcgc tcggcgaatc gcgcaacacg gcagcgatgc gcgccggcgc gcgcgccggg
     1201 atcaggaacg tgatcgatct cgcgtaccgg ctcggcatgc cgcgcgatcc gaggcacatg
     1261 ctccagccgt atccgacgac cgcgatcgga gcgtcggaag tgaatccgct ggcgatggca
     1321 acgaccgccg cgttcgtcaa cggaggcttt cgcgtgacgc cccgcttcgc gaacgacgta
     1381 tgccacgatg gccgctcgct cctctacgac gacgataccg gcaagccgaa ggcttgcgac
     1441 atcaagggcg agcaccacgc gacgcaggag cggctcatgc atccggccgt ttccaatgcg
     1501 atgatcgagc tcctgaaggg cccgctcgat atcggcccga ccggcaccgc cgccggcctc
     1561 cgcacgggcg tcatccccgg catggatccg ctgagcgacg cggtctggaa gctcacgccg
     1621 cagcaacgca agaagcgcac gttgtcgttt ccgctcgatc tggcgggaga gatagccgga
     1681 aagaccggta cggcgacgaa tgcggacggc aaaacgtccg atgtgtggct gatgctgttc
     1741 gttcccggcc cccccgaaca tccggagaag ggcgtggtgc tcggcttctg gatgggcaag
     1801 gattcgaagg accacccgct cggcagtcgc ggggcgaccg gcggcccggg attcgccgag
     1861 tcgggcgcgc gcaactggac gcactcggcg gccacggtgc tcgccttcct gcagaaggag
     1921 cgcggcctgc tgcgccccgg ctaccggttc cgtccgatca tccgcgacga cgtgcagcag
     1981 caggtcgacg cgaagacggc ggagcccgac gcgatcgttc cggttgccgc ggtgggcccg
     2041 ctggtggcag cgtcgaccgc gaagccggcg cccgaccccg cgcgtccgta gctcgcagcg
     2101 cccgcgttcg gcgcacgagc gtcgcgcgca tccgaagcgg acctttgcgc cgtcagctcg
     2161 actgctcgtg gcgcgtcctg cacgtcagca gcacttcgtc gccgttgcgc ttccaccagt
     2221 tgttcgccga gaagatttcc acttcgtgca tgccgcgata gccggcggcc tccatccacg
     2281 agcggatgag gcgaaggtcg atgacgccgt cgcccatcat gccgcggtcg ttcagcaggt
     2341 cggccgtcgg cacgagccag tcgcagatgt gataggcgta gaggcgatcg tgcgcacccg
     2401 cacgctcgat ctctgcacga agctcggggt cccaccacac gtgatacacg tcgacggcga
     2461 tgccgaggcc gtccttgccg ttcggcgaaa gctcgtcgca aagcgcgttg gcgtgcgcca
     2521 tcgtgttcac gcacgcgcgg tcggccgcat acatgggatg cagcggctcg atggccagcg
     2581 gcatgccggc gccgcgcgca tagtcgagga gctcgccgat gccgtcacgc accatttcgc
     2641 gcgcaccggc gatgtcgttc gacacgatcg cgccgcgcgc atccttcggc agcccgccga
     2701 cgacgagcac caggcactgc gcaccgagcg ccgccgcttc gtccaccgca cgccggttgt
     2761 cgtcgagcgc ggcacggcgc gcctgccgat cgggtgcggg aaacatgccg ccacggcaaa
     2821 ggccgctcac ggtgagaccc gcatcgcgga tctgctgcgc ggttgccttg agcccggcct
     2881 tcgcgacctg gtcgcgccac ggagcaatgc cgcgaatgcc gtggcgcgcg aggccggcaa
     2941 tgatttcggg cagcgtcca
//
LOCUS       DASSVX010000009         4985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_650138, whole genome shotgun sequence.
ACCESSION   DASSVX010000009 DASSVX010000000
VERSION     DASSVX010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650138"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFO53_00510"
     CDS             complement(<1..609)
                     /locus_tag="VFO53_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET8874782.1"
                     /translation="MQNLLDLEPAALAHWFAGRGEKPFRARQVCRWVHRRFAADISAM
                     SDLAKPLRASLSGAAEIRAPSVLRDTTAADGTRKWLLDAGNGNAVEAVYIPEDDRGTL
                     CISSQAGCALDCAFCSTGRQGFNRNLTTGEIVGQLWHANRTLLNDGIEAPWVDAGRAP
                     ITNVVMMGMGEPLANFDNVVPALRTFLDDNAYGLSRRRVTLST"
     gene            complement(630..1055)
                     /gene="ndk"
                     /locus_tag="VFO53_00515"
     CDS             complement(630..1055)
                     /gene="ndk"
                     /locus_tag="VFO53_00515"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET8874783.1"
                     /translation="MAVERTLSIIKPDAVAKNVIGEIYSRFEGAGLMIVAARMAWLSR
                     RDAEGFYAVHRERPFFRDLVDFMTTGPVMIQVLEGDNAIAKNRELMGATDPTKAAAGT
                     IRADFARSIDANAVHGSDAPDTAANEIAYFFPAYSIYAR"
     gene            complement(1197..2552)
                     /gene="tilS"
                     /locus_tag="VFO53_00520"
     CDS             complement(1197..2552)
                     /gene="tilS"
                     /locus_tag="VFO53_00520"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET8874784.1"
                     /translation="MADSRRSLPADGRAHDGVGHAVADLVAQALPGGGRVAVALSGGR
                     DSIVLLDAASRITARARIALIAFHVHHGLSPNADRWSLFCRDACAERAIAFVSRHVCV
                     TRAARVSVEAAARKLRYDTLAALAQAHAVDAVLLGHHADDQAETVLLQLLRGAGPRGL
                     AAMPAIAIDRGVRWLRPFLAMTRQTLDAEVAARRLAYIDDESNADARYRRNALRAGVV
                     PALRAMAPGYPETLVRAARHQAEAAALLDELAALDARGACDGATLDRAALRSLRPPRA
                     RNLLRWFLRERGLPAPSSARLGEMLRQLTSAGDDARTTLVHAGAELGVHRGRVCVHRR
                     APGAYRLEWSGDGSIALPHGTLVFDAAAGEGIAARHLAASRVTLRSGTPGERLRAGGH
                     VRRGVADLLREAGVPEWARIGVPRVYCGDALAAVACAGVDAAFAAAPGEPAVALGWHP
                     D"
     gene            complement(2521..3489)
                     /locus_tag="VFO53_00525"
     CDS             complement(2521..3489)
                     /locus_tag="VFO53_00525"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HET8874785.1"
                     /translation="MKQTFLDFEQPIAELQQKIDELRYVHEDSAVDISDEIARLTKKS
                     QQLTKDIYSKLTAWQIAQVARHPQRPYTRDYISGMFGDFRELHGDRSYADDPAIIGGL
                     ARFNGHPCMVLGQQKGRDTKEKIHRNFGMPRPEGYRKALRLMKLAEKFGLPLFTFVDT
                     PGAYPGIGAEERGQSEAIGRNLYEMAGLRIPIITTVIGEGGSGGALAIAIGDVTLMLQ
                     YSTYSVISPEGCASILWKSAEHASAAAETLGITAARLKQLGLVDKVVNEPTGGAHRDH
                     AATMVTLKKALTETLRQLQEHTIEALLETREDKILAYGRFKEIAPG"
     gene            complement(3611..4054)
                     /locus_tag="VFO53_00530"
     CDS             complement(3611..4054)
                     /locus_tag="VFO53_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HET8874786.1"
                     /translation="MSERRYWIGVVAQDHVEAAVAHGFVQLNYGKAAPLARMQPGDGL
                     AVYSPRATFPDGAPLRAFTAIGHVGDGPIFEVPPDEPAVICRRSAAWLDATPAPIKPL
                     LGELSFIRNKEHWGAAFRFGVLRVPRDDFLAIAAAMGRNPAIDFA"
     gene            4213..>4985
                     /locus_tag="VFO53_00535"
     CDS             4213..>4985
                     /locus_tag="VFO53_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET8874787.1"
                     /translation="MHRIAVIPGDGIGKEVVPEGLRVLEKVAGKFGIGLEFVHFDWSC
                     DHYAKTGRMMPDDWFETLSSFEAIFYGAVGWPATVPDHVSLWGSLIQFRRRFDQYVNL
                     RPCRLMPGIATPLANRKPGDIDFVVVRENTEGEYSSLGGRLFEGTEREFVVQESVFTR
                     KGVDRILKYAFELAASRPERHLTSATKSNGISITMPYWDERFRAMAASHPGIRVDQFH
                     IDILCAHFVQRPQTFDVVVGSNLFGDILSDLGPAVCGTIG"
BASE COUNT          801 a         1658 c         1726 g          800 t
ORIGIN      
        1 cgtcgacagc gtgacacgcc gtcgcgaaag tccgtaggca ttgtcgtcga ggaacgtgcg
       61 cagcgcggga acgacgttgt cgaagttcgc cagcggctcg cccattccca tcatcaccac
      121 gttggtaatc ggcgcccggc cggcgtcgac ccatggcgcc tcgataccgt cgttcaacag
      181 cgtgcggttc gcatgccaca actggcccac gatctcgccg gtggtgaggt tgcggttgaa
      241 tccctgccgc ccggtggagc agaacgcgca gtcgagcgcg cagccggcct gcgacgagat
      301 gcacagcgtc ccgcggtcgt cctcggggat gtagacggcc tcgacggcat tgccgttacc
      361 ggcgtcgagc agccatttgc gcgtgccgtc ggcagcggtg gtgtcgcgca gcacggatgg
      421 cgcgcgaatt tcggcggcgc cggacagcga cgcgcgaagc ggcttcgcga gatcgctcat
      481 cgcacttatg tcggcggcga aacgcctgtg cacccagcgg cagacctggc gcgcgcgaaa
      541 cggcttttcg ccgcgcccgg cgaaccagtg ggcgagcgcc gcgggttcga ggtcgagcag
      601 gttctgcatc gcgacgcgtc gtcctccgat taccgtgcgt agatgctgta tgccgggaag
      661 aaataggcga tttcgttcgc cgccgtgtcc ggcgcgtcgg agccgtgcac ggcgttggcg
      721 tcgatcgacc gcgcgaagtc ggcgcgaatg gtgccggcgg ccgccttcgt cggatcggtg
      781 gcgcccatca gctcgcgatt tttcgcgatg gcgttgtcgc cttcgagcac ctggatcatc
      841 accgggcccg tcgtcatgaa atcgacgagg tcgcgaaaga acgggcgctc gcgatgcacc
      901 gcgtagaagc cttcggcgtc ccggcgtgac agccacgcca tgcgcgctgc cacgatcatg
      961 agtccggccc cttcgaagcg cgagtagatc tcgccgatga cgttcttcgc cacggcgtcg
     1021 ggcttgatga tcgaaagggt gcgctcaact gccatgtttc cgctccgaaa agataaaagc
     1081 aagtaaattc aaaattataa catgcgaggc cgtcgcgatt ggcatgcaat cgggcgccgc
     1141 gcggagcgca ttgaatcggc cgttcgacga tcgctcccgg gacgccgccg gacgcgtcag
     1201 tcggggtgcc atccgagcgc caccgcgggc tcgcccgggg ccgcggcgaa tgccgcatcg
     1261 acgccggcgc acgccacggc ggccagcgca tcaccgcagt agacgcgcgg cacgccgatg
     1321 cgcgcccatt ccggcacgcc cgcttcgcgc aagagatcgg caaccccccg gcgcacgtga
     1381 ccgccggccc gcaagcgctc tcccggcgtg ccggagcgca gcgtgacgcg agacgcggcg
     1441 agatggcgcg ccgcgatgcc ttctccggcg gccgcgtcga agacgagcgt gccgtgcggc
     1501 aatgcgatgc tgccgtcgcc cgaccattcg agccgatacg cgccgggcgc gcgccggtgg
     1561 acgcagacgc gtccacggtg cacgccgagc tcggcgcccg catgcacgag cgtcgtgcgc
     1621 gcgtcgtcac cggccgacgt gagttgccgc agcatctcgc cgagccgcgc cgaggagggc
     1681 gccggcaagc cgcgctcacg caggaaccag cgcaggaggt tgcgtgcacg cggcgggcgg
     1741 agcgaccgga gggcggcgcg atcgagcgtg gcgccgtcgc atgccccgcg cgcatcaagg
     1801 gccgcgagct cgtcgagcag cgcggcggct tccgcctgat ggcgcgccgc gcgcacgagc
     1861 gtttcggggt agcccggcgc catcgcgcgc aacgccggca cgacgcccgc gcgcagcgcg
     1921 ttgcggcgat agcgcgcatc ggcgttgctt tcgtcgtcga tgtaggcgag tcgccgcgcg
     1981 gcgacctccg cgtcgagcgt ttggcgcgtc atcgcgagga acggccgcag ccagcgcacg
     2041 ccgcgatcga tggcgattgc cggcatcgcg gcaaggccgc gcggcccggc gccgcgcagc
     2101 aattgcagca gcacggtttc ggcctggtcg tcggcgtggt gaccgagcag gacggcgtcg
     2161 accgcatgcg cctgcgcgag cgctgcaagc gtgtcgtagc gaagtttcct cgccgccgct
     2221 tcgacgctga cgcgcgcagc gcgcgtcacg cacacgtgcc gggagacgaa ggcgatggcc
     2281 cgttccgcgc acgcatcgcg acagaacagc gaccaccgat ccgcgttcgg cgacaggccg
     2341 tgatgcacgt ggaaggcaat gagtgcaatg cgcgcacgcg cggtgatgcg cgacgcggcg
     2401 tcgagaagca cgatcgagtc gcgcccgccg gaaagcgcga ccgcgacgcg gccgccaccg
     2461 ggcagcgcct gcgcaacaag gtcggcgacc gcgtggccga cgccatcgtg cgcgcggccg
     2521 tcagccggga gcgatctcct tgaatcggcc ataagcgagg atcttgtctt cgcgtgtctc
     2581 gagcaaggcc tcgatcgtgt gctcctgcag ttggcgcagg gtttcggtca acgccttttt
     2641 cagcgtaacc atcgtcgcgg cgtgatcgcg atgcgcgccg ccggtgggct cgttcacgac
     2701 cttgtcgacc agcccgagct gcttcagtcg cgcggcggtg atgccgagcg tttcggcggc
     2761 ggcagatgcg tgctcggccg atttccagag gatcgacgcg caaccttcgg gcgagatgac
     2821 cgaataggtc gagtattgca gcatcagcgt cacgtcgccg atcgcgatgg cgagcgcgcc
     2881 tcccgagccg ccctcgccga tcaccgtggt gatgatcggg atgcgcagac cggccatttc
     2941 gtaaagattg cggccgatgg cctccgactg cccgcgctcc tcggcaccga tgcccggata
     3001 cgcccccggc gtatcgacga acgtgaacag cggcaatccg aacttctcgg cgagcttcat
     3061 gagccgcagc gccttgcggt agccctcggg acgcggcatg ccgaaattgc ggtggatttt
     3121 ttccttggtg tcacggccct tctgctggcc gagcaccatg caggggtggc cgttgaagcg
     3181 cgccaggccg ccgatgatcg ccggatcgtc ggcatacgag cgatcgccgt gcagctcgcg
     3241 gaaatcgccg aacatcccgc tgatgtagtc gcgcgtgtac gggcgctgcg gatggcgcgc
     3301 gacctgcgcg atctgccacg cggtgagctt gctgtagatg tccttcgtga gctgctggct
     3361 tttcttcgtc aggcgcgcga tctcgtcgga gatgtcgacg gccgagtcct cgtggacgta
     3421 gcggagctcg tcgatcttct gttgcagctc ggcgatgggc tgttcgaaat cgaggaaggt
     3481 ctgtttcatg cggtgcgtgc ggggaaggct ctcggcatga tagcgcagcg cggcacggcg
     3541 cccggtgtgc agggcgcgcc ggcgcgaacg gaagcgggtt cggggcacat cgttcgcgac
     3601 gcggtacggc tcaagcgaaa tcgatcgccg gattgcggcc catcgccgct gcgatggcga
     3661 ggaaatcatc gcgcggcacg cgcagcacgc cgaagcgaaa cgcggccccc cagtgctcct
     3721 tgttgcgaat gaacgagagc tccccaagaa gcggcttgat gggggcaggc gtcgcgtcga
     3781 gccatgcggc gctgcgccgg cagatgaccg cgggttcatc gggcggtacc tcgaagatcg
     3841 gtccatcgcc cacgtggccg atcgccgtga acgccctcag aggcgcgcca tcggggaacg
     3901 ttgcgcgagg cgagtagacg gcgagaccgt cgcctggctg catgcgcgcg agcggtgcgg
     3961 ccttgccgta gttgagttgc acgaagccgt gcgcaaccgc ggcctcgacg tgatcctgcg
     4021 cgacaactcc gatccagtac cggcgctcgc tcatcgcgat tcctttccgt gtgtcgaaag
     4081 tgcttgcgtg cacacggtaa cgagcgccgc tcgagcacgc catcgaacga atggatgagc
     4141 cgaagcgcgt tgcgcgccgc cgcgcatcct ataatcggcg ttcgacacga ggggtaatac
     4201 gaggggtaat ccatgcaccg aattgcagtc atcccgggcg acggcatcgg caaggaggtg
     4261 gttcccgagg gcctgcgcgt gctcgagaag gtcgccggca agttcggcat cggtctcgaa
     4321 ttcgtgcatt tcgactggag ctgcgaccac tacgccaaaa cgggccgcat gatgcccgac
     4381 gactggttcg agacgctgtc ctcgttcgag gcgatcttct acggcgccgt cggctggccg
     4441 gccaccgttc ccgatcatgt gtcgctgtgg ggatcgctga tccagttccg ccgccgcttc
     4501 gaccagtacg tgaacctgcg cccgtgccgg ctgatgcccg gcatcgcgac gccgctggcc
     4561 aatcgcaaac ccggcgacat cgacttcgtc gtcgtgcgcg aaaacaccga aggcgaatac
     4621 tcgtcgctcg gcgggcgtct gttcgaggga accgagcgcg agttcgtcgt ccaggaatcg
     4681 gtgttcacgc gcaagggcgt cgaccgcatc ctgaagtacg cgttcgagct cgcggcatcc
     4741 cgccccgagc gtcacttgac gtcggccacg aagtcgaacg gcatctcgat cacgatgccg
     4801 tactgggacg agcgcttccg ggcgatggcg gcaagccatc ccggcatccg cgtcgaccag
     4861 ttccacatcg acatcctctg cgcgcatttc gtgcagcgcc cgcagacgtt cgacgtcgtc
     4921 gtcggctcga acctgttcgg cgacatcctg tcggacctgg gaccggccgt gtgcggcacg
     4981 atcgg
//
LOCUS       DASSVX010000010         7601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_516817, whole genome shotgun sequence.
ACCESSION   DASSVX010000010 DASSVX010000000
VERSION     DASSVX010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516817"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..1335)
                     /locus_tag="VFO53_00540"
     CDS             complement(271..1335)
                     /locus_tag="VFO53_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET8874788.1"
                     /translation="MKITTLGIDLAKNVFQLHGVDERGKVLLKKQLKRTQMLPFFANL
                     PPCLIGMEACASAHYWARKLAPLGHTVKLMAPQYVKPYVKTNKHDAADAEAICEAVGR
                     PSMRFVAVKSTEQQAILAVHRARQGFVKARTAQANQIRGLLAEFGVVLPQGITHISER
                     LPAILEDGDNALPGSFRQVLARLGEHLQHLSTHVAALEREIHRWHRDSEASQRLAAIP
                     GIGPLGASALVATVAAPTSFANGRQLAACLGMVPRQASSGGKPTLLGMSKRGDSYLRT
                     LLIHGARSVLRQVERHAEHADPWLRQLLRRRPKNVVAVALANKNARIAWALLAHGRTY
                     QPQYSAAARGCSGAPDEMIS"
     gene            1620..2255
                     /gene="purN"
                     /locus_tag="VFO53_00545"
     CDS             1620..2255
                     /gene="purN"
                     /locus_tag="VFO53_00545"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET8874789.1"
                     /translation="MPLTRITVLISGRGSNLAALIDAANAGAIDGAVTHVISNRPDAG
                     GLAHAERNGIATTVVDHRAFAARDEFDAALAAAIDAGEPELIVLAGFMRILGAAFVQR
                     YAGRMLNIHPSLLPAYPGTGTHGRALADRATRHGCTVHLVTPDVDGGPIIAQSEVPVL
                     PGDDPATLAARVLEAEHRLLPQVVGWFCAGRVAVEGDRVRITGPSHDAMCR"
     gene            2252..3526
                     /locus_tag="VFO53_00550"
     CDS             2252..3526
                     /locus_tag="VFO53_00550"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HET8874790.1"
                     /translation="MSYAAELGALPSVRRRMKSSARRDAGSGTASVAIASRGGSQSPW
                     HVRPALVVALVLSLLLHLAWSLWPVESTRDVAPEEPALTATLTEMPPPPAPSPPVERV
                     ASPPSVVPKTRPPAPRRAHAPVVAVQRPLAPGNAEIVAPQDALDVTANATPTPAAGEP
                     SLADVAIGPPAADATPPVVLPPRLDLAYKVFFGTQGFMIGEATYRFERDGDRYRISTV
                     AQPRGLAALLVHGKGLVESRGTITPQGLKPYEFVVERGSADKREVAYFDWDAGNVVLN
                     GGDLVPLEAPAFDPLTILWQPYFTPPSRDDQTFSLATTRRVARYTLSLAGEETIAWRN
                     GDVVTQRWHEVSDDGKTEGWFWLAPSMHYIPVKMRVTRTSRGTLEVLLDAIRTDASGA
                     PVVDEPAAADSTSFNAPDPRAPDPGGAESHGQ"
     gene            3647..4906
                     /locus_tag="VFO53_00555"
     CDS             3647..4906
                     /locus_tag="VFO53_00555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HET8874791.1"
                     /translation="MTLRRNQLTALAGAITRVARLDMPADAALSAFFREHAEMGVRDR
                     ALVSDGAFAYLRRKASLEALAQTADPRLLAYAVMVREFGHSVREIEPAINASDARWLA
                     GFKGRLHDPLAPAVAADVPDWLWHRLGETYDDAARTALTRAWQVPAPLDLRANVMKTT
                     RDAALAALSAEGLAVTPTPYAPFGLRIDGRPSLARHAWLADGRLEVQDEGSQLVGLLV
                     APRRSDMVVDFCAGAGGKTLLLGAMMRSQGRLYAFDVSPRRLAHLTPRLARSGLSNVH
                     PQVIASERDTKVKRLAGKIDRVLVDAPCTGFGTLRRNPDLKWRQTPDDLNELTAKQAR
                     ILAAASTLVKPGGRLVYATCSVLPEENDAIVDAFVAAHADFTERDVASLLADARIPLD
                     TGARLRLRPDVHGCDGFFAAVLERKTA"
     gene            4982..6283
                     /locus_tag="VFO53_00560"
     CDS             4982..6283
                     /locus_tag="VFO53_00560"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET8874792.1"
                     /translation="MIAPIDFSRLQVALALPRGWLELLLTLACIGIAWLVDRRLAQHR
                     ARRLPQAHLPGSFIDLGFPLIALLLVYVASIAWRHLVGPPFFLAIATPILIALAVIRM
                     LAYALHRLFPAQTWLPASELAIGTAIWGLAILYFLGVLPEIRATLAQLVIPIGKTDVS
                     LLTIFTGIAVVVVTLIVTLWISGLIEQRLALATQLDANLRVVLAKFIRAVLIVVAVLI
                     ALEQIGFDLTLLTVFGGALGVGIGLGLQKLASNYIAGFTILLDRSIRIGDLVTVDGRT
                     GVVSNATSRYVVVRSGDGVEAIVPNETVVTTTVLNHSYTSPQVRLALQVQVAYDSDVE
                     KALALLVEIALREPRVLRDAQAPKSFLVNFADNGITLELGVWINDPHVGQLDLKSSLN
                     RAILKAFGANGIQIPFPQRDIRIVGAIPAQPDNAKIPESPP"
     gene            6515..>7601
                     /locus_tag="VFO53_00565"
     CDS             6515..>7601
                     /locus_tag="VFO53_00565"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET8874793.1"
                     /translation="MNSDFLALAAHGLVDLPWWGYVVVALVLTHVTIASVTIFLHRSQ
                     AHRALDLHPIVAHFFRFWLWITTGMVTKEWVAIHRKHHAKVETDDDPHSPQTRGIETV
                     MWRGAELYRDEAKNRETLDKYGHGTPDDWIERNLYTRFSWQGVGVMMVIDLALFGPIG
                     LTIWAVQMLWIPITAAGIINGIGHYWGYRNFACLDASTNIVPWGIVIGGEELHNNHHA
                     YGSSAKLSSRWYEFDIGWMYIRILEAFKLATVRKIAPQIKFDGSKAVADLATLQAIIT
                     HRYAVATSYAKSVKAACATEIAALRARHGHVDLPSLRRVKRWLASDPVALPEGERARL
                     EQTIEKSTVLQQIYHMRLELAALWSRST"
BASE COUNT         1192 a         2675 c         2411 g         1323 t
ORIGIN      
        1 cgtcgttccc gcggaagcgg gaacccatac atggtcgccc cctttttgcc aagcatccga
       61 tttgcgatag cgagcaggta gtgattgcag ccatacatcc ggcctttaga tcgaggccgc
      121 aacggcctct ggccctgatg gaattcgccg atcgggagct catcagcgtc atgcactcga
      181 agtgcgctgg gttccgcagc tttgcccgat cgcggtccga cctgttttgc catcacgtca
      241 cgtttgcctg agcaatcagt ggtctctact tcagctaatc atttcgtccg gggcaccgct
      301 gcaaccacgg gcagcggccg aatactgcgg ctgataggta cgtccatgcg ccaacaacgc
      361 ccatgcgatg cgggcgtttt tgtttgccag cgcgaccgcg actacgttct tcggtcgacg
      421 cctcagcagt tgccgcagcc acgggtcagc gtgttcggcg tgccgttcaa cttggcgcag
      481 caccgagcgg gcaccgtgga taagcagcgt gcgcaggtaa ctatcgccgc gcttgctcat
      541 gccgagcagc gtcggcttgc cgccgctgga agcctgccta ggtaccatcc ccaggcaggc
      601 ggccagttgt cgtccgttgg caaaactcgt gggcgcagcg accgtcgcca ccaaggcgct
      661 ggcaccgagc ggaccgatgc ccgggatcgc cgccagccgt tgactcgctt cgctgtcgcg
      721 gtgccagcgg tgaatctcac gctcaagagc ggcgacgtgc gtgctcaagt gttgcaaatg
      781 ctcgcccagg cgggccagca cttgccggaa actgcccggc aacgcattgt cgccatcctc
      841 gagaatcgcc ggcaagcgct cgctaatgtg cgtgatgcct tgcggcagca ccaccccaaa
      901 ctcggccagc agcccgcgga tctgattggc ctgcgcggtg cgcgccttga cgaagccttg
      961 ccgggcccga tgcacggcca ggatcgcttg ttgctccgtg ctcttgaccg ccacgaagcg
     1021 catgctcggc cggcccaccg cttcgcagat cgcctcggcg tcggccgcat cgtgcttgtt
     1081 ggtcttcacg tacggcttga cgtattgcgg ggccatcaac ttcaccgtgt gcccgagcgg
     1141 ggccagcttg cgcgcccagt aatgcgcact cgcgcacgcc tccatgccaa tcagacacgg
     1201 cggcagattg gcgaagaacg gcagcatctg cgtgcgcttg agctgcttct tcagcaacac
     1261 cttgccgcgt tcatccacgc cgtgcaactg gaacacattc tttgccaaat cgatgcccag
     1321 cgtcgtaatc ttcatagcgg tcgcctcctc tcgtgtcagt ggatatgtcc acatttccac
     1381 tgtggcactc tgatgccgca tcgggaaggg ggcgaccatc ccattagtgt ctttcgctcg
     1441 aagcgctact gggtcccccc ttcgcgggga cgacgaagct tcgggtgcct gacgtcgcgc
     1501 aattatgtaa agctcaataa atggggtcag cgccgacttt tcgctgctgc gggttgagat
     1561 cgatcgcgcg caaaagtcgt ctctgacccc acttatccca cttatcgtca ctgaccccga
     1621 tgccgctcac gcgcatcacc gtgctcatct ccggccgcgg ctcgaatctc gccgcactga
     1681 tcgatgccgc gaacgcgggc gcgatcgatg gcgccgtcac gcatgtgatc agcaaccggc
     1741 ccgatgcggg ggggctcgcg catgccgagc gcaacggcat tgcgaccacc gtcgtcgatc
     1801 atcgtgcgtt tgccgcgcgc gacgaattcg atgcggcact cgccgccgcg atcgacgcgg
     1861 gcgagcccga gctgatcgtg ctcgccggct tcatgcgcat tctcggcgcc gcgttcgtgc
     1921 agcgctacgc cgggcgcatg ctcaacatcc acccgtcgct tttgcccgcg tatccgggca
     1981 ccggcacgca tggccgtgcg ctcgccgatc gcgcgacgcg acacggctgc accgtgcacc
     2041 tcgtcacgcc cgacgtcgac ggcgggccga tcatcgcgca gtcggaggtg ccggtgctgc
     2101 ccggcgacga cccggcgacg ctcgccgcgc gcgtgctcga agccgagcat cggctgctgc
     2161 cgcaggtggt cggctggttc tgtgccggcc gcgtcgccgt cgaaggcgac cgcgttcgca
     2221 tcaccggtcc gagccacgat gcgatgtgcc gatgagctac gcggccgaac tcggcgcact
     2281 gccctcggtc cgtcgaagga tgaagtccag cgcgcgccgc gacgcagggt cggggacggc
     2341 ctccgtcgcg atcgcatcgc gtggcggttc gcaatcgccg tggcacgtcc ggcccgcgct
     2401 cgtcgtcgcg ctcgtgctgt cgttgctgct gcatctcgcg tggtcgctgt ggccggtcga
     2461 gtcgacgcgg gacgtcgcgc cggaagagcc ggcattgacc gcgacgctga ccgaaatgcc
     2521 gccgccgccc gcaccctcgc cgcctgtcga gcgcgtcgct tcgccgccga gcgtcgtccc
     2581 gaagacgcgt cctccggcac cgcgccgcgc gcatgcaccg gtcgtcgccg tgcaaaggcc
     2641 gttggcgccc ggcaacgcgg agatcgtggc gccgcaagac gcactcgatg ttacggcgaa
     2701 tgcgacaccg acgccggccg caggcgaacc gtcgcttgcc gacgtcgcga tcggtccgcc
     2761 ggccgccgat gcaacgccac cggtcgtgtt gccgccgcgg ctcgacctcg cgtacaaggt
     2821 tttcttcggc acgcagggct tcatgatcgg cgaggcgacg tatcggttcg agcgcgatgg
     2881 cgaccgttac cggatctcga cggtggcgca gccgcgcgga ctggccgcgc tgctcgtgca
     2941 cggcaagggc ctcgtcgaaa gccgcggcac gatcacgccg cagggcctca agccgtacga
     3001 attcgtcgtc gagcgcggca gcgccgacaa gcgcgaggtt gcgtatttcg attgggacgc
     3061 gggcaacgtc gtgctgaacg gcggcgatct cgtgccactc gaagcacccg cgttcgatcc
     3121 gctgacgata ctgtggcagc cgtatttcac gccgccttcg cgcgacgacc agacgttcag
     3181 cctcgcgacg acgcggcgtg tggcgcggta cacgctgtcg ctcgccggcg aggaaacgat
     3241 cgcctggcgc aacggcgatg tcgtcacgca gcgctggcac gaggtgagcg acgacggcaa
     3301 gaccgaaggc tggttctggc tcgcgccgtc gatgcattac atcccggtga agatgcgcgt
     3361 gacgcgcacg tcgcgcggca cgctcgaggt gctgctcgat gcgatccgca ccgatgcgag
     3421 cggcgcaccg gtggtcgatg aacccgccgc cgccgacagc acgtcgttca acgcgccgga
     3481 cccgcgcgcg cccgatcccg gcggcgccga atcgcatggg cagtgacatc cccccacgct
     3541 tgcggcttcg ccgctacgct gccccccagg gggcgcaatt cgcggcttgg gacggcccgg
     3601 cgccgcgaat gacatcccgc tacgcctgcg gctcccgcac tgagcgatga cgctccgccg
     3661 caaccagttg acggcactcg ccggcgcgat cacgcgcgtc gcgcgtctcg acatgccggc
     3721 cgatgccgcg ctgtcggcgt tcttccgcga acacgccgaa atgggcgtgc gtgaccgcgc
     3781 gctcgtgtcg gacggtgcgt ttgcctatct gcggcgcaag gcgtcgctcg aagcgctggc
     3841 gcagaccgcc gatccgcgcc ttctcgccta cgcggtcatg gtgcgcgagt tcggacactc
     3901 ggtgcgcgag atcgagccgg cgatcaacgc cagcgacgcg cgctggctcg ccggcttcaa
     3961 gggccgatta cacgatccgc ttgcacccgc cgtcgccgcc gatgtgcccg actggctgtg
     4021 gcatcgcctc ggcgaaacgt acgacgacgc ggcacgcacc gcgctcacgc gcgcatggca
     4081 ggtgcccgca ccgctcgatc tgcgtgcgaa cgtgatgaag acgacacgcg atgcggcgct
     4141 ggcggcgttg tcggccgaag gcctcgcggt gacgccgacg ccttacgcgc cgttcggact
     4201 gcgcatcgac ggccggccgt cgcttgcacg tcacgcgtgg cttgccgacg ggcggctcga
     4261 agtgcaggac gaaggcagcc agctcgtggg cctgctcgtc gcgccgcgac gaagcgacat
     4321 ggtcgtcgac ttctgcgccg gtgccggcgg gaagacgctg ctgcttggcg cgatgatgcg
     4381 ctcgcaggga cgcctgtacg cgttcgacgt gtcgccaagg cgcctcgcgc acctgacgcc
     4441 gcgcctcgcg cggtccggat tgtccaacgt gcatccgcag gtgatcgcca gcgagcgcga
     4501 cacgaaggtg aagcggctcg ccggcaagat cgatcgcgtg ctggtcgatg cgccctgcac
     4561 gggtttcggc acgctgcgcc gcaatcccga cctcaaatgg cggcagacgc ccgacgacct
     4621 gaacgagctc accgcgaagc aggcgcgcat cctcgccgcc gcgtcgacgc tcgtgaagcc
     4681 cggcggccgg ctcgtgtatg cgacgtgtag cgtgctgccc gaagagaatg acgccatcgt
     4741 cgacgccttt gtcgccgcgc acgcagattt caccgaacgc gacgtcgcgt cgctgctggc
     4801 cgacgcccgc atcccgctcg acaccggcgc gcggctgcgg ctgcgccccg acgtgcacgg
     4861 ctgcgacggc tttttcgccg ccgtgctcga gcgcaagacc gcgtaatcgg ggtcagagct
     4921 gtaattattc ggccgatgga ataattacag ctctgacccc gattacactt gcgcgaccaa
     4981 aatgatcgcg cccatcgact tcagccgcct gcaggtggcg ctcgcgctgc cgcgcggctg
     5041 gctcgagctg ctgctcacgc tcgcatgcat cggcatcgcg tggctcgtgg atcggcgcct
     5101 cgcgcaacac cgcgcacgcc gcctgccgca ggcgcacctg ccgggcagct tcatcgacct
     5161 cggctttccg ctgatcgcgc tgctgctcgt ctatgtcgcg tcgattgcat ggcgtcacct
     5221 cgtcggtccg ccgttcttcc tcgccatcgc aacgccgatc ctcatcgcac tcgcggtgat
     5281 ccgcatgctt gcgtatgcgc tgcaccggct gttcccggcg cagacgtggc tgcccgcctc
     5341 ggagcttgcc atcggcaccg caatctgggg tctcgcgatc ctctacttcc tgggcgtgct
     5401 gcccgagatc cgcgcgacgc tcgcccagct cgtgatcccg atcggcaaga ccgacgtgtc
     5461 gctgctcacg atcttcaccg gtatcgcggt cgtcgtggtg acgctgatcg tgacgctgtg
     5521 gatttccggc ctgatcgagc agcgcctggc actcgccacg cagctcgatg cgaacctgcg
     5581 cgtggtgctc gcgaagttca tccgcgcggt gctgatcgtc gtcgccgtgc tgatcgccct
     5641 cgagcagatc ggcttcgacc tcacgctgct caccgtcttc ggcggcgcac tgggtgtcgg
     5701 catcgggctc ggcctgcaaa agctcgcgtc gaactacatc gccggcttca ccatcctgct
     5761 cgaccgctcg attcgcatcg gcgacctcgt caccgtcgat ggccgcaccg gcgtggtgtc
     5821 gaacgcaacg tcccgctacg tcgtggtgcg cagcggcgac ggcgtcgagg ccatcgtgcc
     5881 gaacgaaacg gtggtgacga ccaccgttct caaccattcg tacacgtcgc cgcaggtgcg
     5941 tctcgccctg caggtgcagg tggcctacga cagcgacgtc gagaaggcgc ttgccctgct
     6001 cgtcgaaatt gcgctgcgcg agccgcgggt gctgcgcgac gcgcaggccc cgaagtcgtt
     6061 cctcgtcaac ttcgccgaca acggcatcac cctcgagctt ggcgtatgga tcaacgaccc
     6121 gcacgtcggc cagctcgatc tcaagagctc gctcaaccgg gcgatcctga aggcgttcgg
     6181 cgcgaacggc atccagattc ccttcccgca acgcgacatt cgcatcgtcg gcgcgattcc
     6241 tgcacagccc gacaacgcaa agattcccga gtccccgccg taaaccacgg cacttcggac
     6301 gcgcgacgcg ggtcgaaaac acagaagtac cgcccgttgg ccgatccatg gattcacgcc
     6361 ggacgccgct cgcaatttcc ccgcccttgc cccttgcgca tgccgccggc cggggccata
     6421 tcgtcccgaa cgctgctcgc gtacaatggg ccacgcaacc tgcccgccga cgacgccgtc
     6481 gtcgcgcccg cggttgcatt ttttcggact ttcaatgaat tcggattttc tggccctcgc
     6541 cgcgcacggg ttggtcgacc ttccctggtg gggctacgtg gtcgtcgcgc tggtcctcac
     6601 gcacgtcacc atcgcttccg tcacgatctt cctgcatcgc agccaggcgc accgcgcact
     6661 cgacctgcat ccgatcgtcg cgcacttttt ccgcttctgg ctctggatca ccaccggcat
     6721 ggtcaccaag gagtgggtgg ccatccatcg caagcaccac gcgaaggtgg aaaccgacga
     6781 cgacccgcac agcccgcaaa cgcgcggcat cgaaaccgtg atgtggcgcg gcgccgagct
     6841 ctatcgcgac gaggcgaaga accgcgaaac gctcgacaag tacggtcacg gcacgcctga
     6901 cgactggatc gagcgcaatc tctacacgcg cttctcgtgg cagggcgtgg gcgtcatgat
     6961 ggtgatcgat ctcgcgctgt tcggcccgat cggcctcacc atctgggccg tgcagatgct
     7021 gtggatcccg atcaccgcgg ccggcatcat caacggcatc ggccactact ggggctatcg
     7081 caacttcgcc tgcctcgatg cgtcgacaaa catcgtgccg tggggcatcg tcatcggcgg
     7141 cgaggaattg cacaacaacc accacgcgta cggctcgtcg gcgaagctct cgtcgcgctg
     7201 gtacgagttc gacatcggct ggatgtacat ccgcattctc gaagcgttca aattggccac
     7261 ggtgcgcaag attgcgcccc agatcaagtt cgacggctcg aaggcggtgg ccgatcttgc
     7321 gacgctgcag gcgatcatca cgcatcggta cgcggtggcc acgagctatg cgaagtcggt
     7381 gaaggccgcc tgcgccaccg aaattgccgc gttgcgcgca cgtcacggcc atgtcgacct
     7441 gccgagcctg cggcgcgtca agcgctggct cgcgtccgac cccgtggcgc tgcccgaggg
     7501 cgagcgtgcg cggctcgagc agaccatcga aaagagcacg gtgctgcagc agatttatca
     7561 catgcgcctg gaactggcgg cgctgtggag ccgctccacc g
//
LOCUS       DASSVX010000011         8176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_783454, whole genome shotgun sequence.
ACCESSION   DASSVX010000011 DASSVX010000000
VERSION     DASSVX010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783454"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..478
                     /locus_tag="VFO53_00570"
     CDS             146..478
                     /locus_tag="VFO53_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874794.1"
                     /translation="MHTTIRALIGAALVVAAFGAAVAKLPPAPPLTDQQKAEKAAKDK
                     STADAEKAALAKAEDKAIENYVANEKAKGKTVNVNMSAGGNTSPSAGKSLSAQPEKAS
                     SAHSPPKK"
     gene            514..927
                     /locus_tag="VFO53_00575"
     CDS             514..927
                     /locus_tag="VFO53_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET8874795.1"
                     /translation="MIRVLLPVDGSDSSARAVQRVASCSQRVAPVEVQLLYVQVDDGT
                     AATVFDEPARNDALEAGNRALQSAADVLARAGVACMSESRKGFVPSVIVEHAKSTNCD
                     AIVMGTRGMGSTQQLLGSIARQVISLSDLPVTLVK"
     gene            complement(1136..2734)
                     /locus_tag="VFO53_00580"
     CDS             complement(1136..2734)
                     /locus_tag="VFO53_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HET8874796.1"
                     /translation="MNRSLQAHGLVAWIVALSLLLAGCASMPPGADYPKIASTALVDP
                     ASTPLGQGVETLAKAHPGLSGFRLFASGSDAFTLRVQMANRAQRTLDVQYFIFKDDDT
                     GQLLMSAMLHAASRGVRVRVLMDDTEARGQDDRVALLAANPNVEVRLYNPFYYRGPIA
                     FVRFAEFALTMSRLDYRMHNKLFVADNAIAVAGGRNVGDEYFDVGDAPQFGDYDVFVM
                     GPIVRDLSKSFDAYWNSSLSIPVQALLGADRTGAKLETMKVQLREHKADMHQSDEERA
                     IRTGKPLAGLLGDDSGMVWAKAEVLADSPDKAKVENDDEPGALFRHRLLEAARDVKSE
                     LLIVSPYFVPGEKGEALLVKLRNRGVRVRVLTNSLLSTDVPAVHAGYRQYRLPLLEAG
                     VDLYEVKPLPGKPHPRGGLLKTPSSGQFSLHAKAFVFDRKRIFIGSANFDRRSLHLNT
                     EIGLMIDSPQLAQQVLARFDTIANPANSFVLALHENAAGTPRIEWKSMKDGKPVVYTS
                     EPGDDRWREFLVDLYGLLPIEDQL"
     gene            2945..3032
                     /locus_tag="VFO53_00585"
     tRNA            2945..3032
                     /locus_tag="VFO53_00585"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2979..2981,aa:Ser,seq:cga)
     gene            complement(3035..3616)
                     /locus_tag="VFO53_00590"
     CDS             complement(3035..3616)
                     /locus_tag="VFO53_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET8874797.1"
                     /translation="MNAIRIIHSEHRSLAAVLHGMLHLVREIGLRNSAPNFELFGAMV
                     YYIDAFAERFHHPKEDEYLFKLLRLRSADATALVDRLATEHRAGAEKIRALEQALRRY
                     EEGGKSEFAAFAEAVRAYAAFHWEHMRAEEDRLLPLARAHLTDSDWRQIDDAFAGNSD
                     PLFNVETGAQYDALFRRIVSLAPPPIGVGPYTR"
     gene            3848..6103
                     /locus_tag="VFO53_00595"
     CDS             3848..6103
                     /locus_tag="VFO53_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET8874798.1"
                     /translation="MEVPAAAFDISREGAGGASPHEHFVQFYETEPALVESIADFVGT
                     GLSAGAAAIVIATQPHLAQLDERCSARGIDLASARGEGRYVVRDARTSVESLLVEGWP
                     DAGRFTATLEPLVAAAARRGSRVVIFGEMVALLWNDGRHDAAVQLEQLWNELAQRYAF
                     SLFCAYPIAQMQSGPWPAMHAVCAAHSNTIPTHATRYADEEKRLSELCDLQRQARALE
                     HEIGQRRATENLLASRERELADILENAAQAIHSVGPDGVILWANQCELDMLGYAASEY
                     VGHPIADFYVDASVAARILRRLGAGESLRDEPVQMRHRNGSVRDVLVTSNVRFEHGAF
                     AQTRCFSRDVTEQRCVERALRESEEHAQHTRSLLASIVESSDDAIISKSLDGRITSWN
                     ASARRVFGYTAEEAVGKPITMIIPPEHQHEERLILERLGRGERIEHFETVRVAKDGRR
                     IDVSLTISPVRDQRGRVVGASKVARDVSERKRMEERLRDADRRKDEFIAMLGHELRNP
                     LAPIRNVAALLRRTTAGDSASAELCLMLERQVQQMTRLLDDLLDVNRITRGNIRFQRE
                     LLDLATVVQHAVEASGPIIDRRGHRLHVDLPAEPLRVNGDAARLVQMLTNLLNNAAKY
                     TPDGGRIELAVARRDASLELRVKDNGVGIALEMLPRVFDLFVQADRSGGDVQEGLGLG
                     LTLVRVIAEHHGGVVEAVSAGPGRGSEFIVRLPDAEASRPVDAARAQGRRSVAVDGHA
                     ARDALRPHPFG"
     gene            6262..6723
                     /locus_tag="VFO53_00600"
     CDS             6262..6723
                     /locus_tag="VFO53_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8874799.1"
                     /translation="MSANVIELRKEARRRVLVAHQRSDVRHALRTLIEQDNVIVVEAA
                     EGDAALAELEFGRFDLLLLELDLPVKDGIAVMQLHRMLLAHEHVHAEPPAVVLTLPPE
                     VRDNAALTDHLRTLGVIAFIDDAPRPGVASLVDDILRARAAESDSHKPAVA"
     gene            6749..>8176
                     /locus_tag="VFO53_00605"
     CDS             6749..>8176
                     /locus_tag="VFO53_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET8874800.1"
                     /translation="MREASESAITAPASSRFGSGKSVKRVEDDALLRGLGRFADNLPA
                     PGQLHTYFVRSPHPHARITRIGSDAALKLPGVAAVFTGRDLAGAGVKPIPTSADYRRA
                     DGSPTASPLRHALAVDTVRFVGEAVVAVVAATLHEARDAADRVEVDYEPLPAIVGVDA
                     AIAPGAPAVVAEAPDNVACEARHGNRRDAEAAFARAAHVVALDLVNQRVAPSPIEPRS
                     TLAMCDAATGRLALRVSCQTPTGLRDELCEVLGIESDKVRVLVGDVGGGFGMKTSLYP
                     EDVAVAFAARKLGRPVKFTAERIEEFLAASHGRDVTSRAELALDGNGRILALRIVSLA
                     NVGAYATPAGVVIQLLIGPWVSTSVYDIRTIDIGIKAILTHTLPTGPYRGAGRPEAIY
                     IIERLIDEAARQTGIDRVELRQRNMIDPAQMPYTNAMDKTYDSGQFEKVMMQALARSA
                     WNDFSARAERSRAEGRWRGRGIATFL"
BASE COUNT         1407 a         2703 c         2746 g         1320 t
ORIGIN      
        1 gtgggcgaag gagcatcacc tgtactggta caacgacgtc aaggccggca agattcccac
       61 ggcgccggac gatgcgccgc cggttcctgc gccgcgcccc gcgtagccgc acagtccatc
      121 cgcaagcgaa ttcggagagc cacgcatgca caccaccatc cgggcattga tcggcgcggc
      181 gctcgtcgtc gcggcattcg gagccgccgt cgccaagctg cctccggcgc caccgctcac
      241 cgatcagcag aaggccgaga aagcagcgaa ggacaagagc acggccgatg ccgaaaaggc
      301 ggcgctcgcc aaggcggagg acaaggcgat cgagaactac gtcgcgaacg agaaggcgaa
      361 gggcaagacg gtcaacgtga acatgtccgc aggagggaac acgtctccgt cggccggaaa
      421 gagtctgtcc gcgcagcccg agaaggcatc cagcgcgcat tcgccgccga agaagtagcc
      481 gttcaccacg aggcgctcgc ccggcggtcg ccgatgatcc gcgtgctgct cccggtcgac
      541 ggctcggata gttccgcgcg tgccgtgcag cgggtggcga gttgttcgca gcgagttgca
      601 cccgtcgaag tgcaattgct ctatgtgcag gtggatgacg gcacggcggc cacggtgttc
      661 gacgaaccgg cgcgcaacga tgcgctcgaa gcaggaaatc gcgcattgca atccgcggcg
      721 gatgtgctcg cgcgcgccgg ggttgcgtgc atgagcgaat cgcggaaggg cttcgtaccg
      781 tcggtgatcg tcgaacatgc gaagtcaacc aactgcgacg ccatcgtgat gggcacgcgc
      841 ggcatgggat cgacgcagca actgctgggc tccattgccc gccaggtgat cagcctctcc
      901 gacttgccgg tgacgctggt gaaatagccg atacggcgcg ctcgcgcgga ggaaaaccat
      961 ggccgaagga catcgcgaga agctgctcga tcaagcgctg gccgacacct ttcccgcaag
     1021 cgatcccgtt tcggccgcgc agccgggggg agggccggac agcggcgcgc ctctaccggg
     1081 cacacccgca cagccgcaaa gcgcttcgca catgcactcg ccgaaccggc cgcgctcata
     1141 gctggtcctc gatcggcagc aggccgtaga ggtcgacgag gaactcgcgc caccgatcgt
     1201 cgccgggctc gctggtgtac acgaccggct ttccatcctt catgcttttc cattcgatcc
     1261 ggggcgtgcc ggcggcgttc tcgtgcaacg cgaggacgaa gctgttcgcc ggattggcaa
     1321 tggtgtcgaa gcgtgcaagc acctgttgcg cgagctgcgg actgtcgatc atcaagccga
     1381 tctcggtatt gaggtgcagc gagcgccgat cgaaattcgc cgaaccgatg aagatgcgct
     1441 tgcggtcgaa gacgaacgcc ttcgcatgca gcgagaactg ccccgagctt ggcgttttga
     1501 gcaggccgcc gcgaggatgc ggcttgccgg gcagcggttt cacttcatag aggtcgaccc
     1561 ccgcctcgag caggggaagc cggtattgcc ggtagccggc atgcacggcg gggacgtccg
     1621 tggacagcag cgaattggtc aggacgcgaa cgcgcacgcc gcggttgcgc agcttaacga
     1681 gcaacgcctc gcccttttca cccgggacga aatacgggga cacgatcagc agctcgctct
     1741 tcacgtcgcg cgcagcttcg agaagccggt ggcggaacaa cgcgcccggc tcgtcgtcgt
     1801 tttcgacctt cgccttgtcc ggactgtcgg ccagcacctc ggccttcgcc cacaccatgc
     1861 cgctgtcgtc gccgagcagc cccgcaagcg gcttcccggt gcggatggcg cgctcctcgt
     1921 cgctctgatg catgtccgcc ttgtgctcgc gaagctgcac cttcatcgtc tcgagcttgg
     1981 cgccggtgcg atcggcaccg agcagcgcct ggaccgggat cgacagcgag ctgttccagt
     2041 aggcgtcgaa gcttttcgac aggtcgcgca cgatcggtcc catgacgaac acgtcgtagt
     2101 cgccgaattg cggcgcatcg ccgacgtcga aatactcgtc gccgacgttg cgcccaccgg
     2161 cgaccgcgat cgcgttgtcg gcgacgaaca gcttgttgtg catccgatag tcgagccggc
     2221 tcatcgtgag cgcgaattcg gcgaagcgca cgaacgcgat cgggccgcgg tagtagaacg
     2281 ggttgtacag gcgcacctcg acgttggggt tcgctgccag cagcgcgacg cggtcgtcct
     2341 ggccgcgcgc ctcggtgtcg tccatcagga cccgcacgcg cacgccgcgg ctcgccgcgt
     2401 gcagcatcgc gctcatcagc aactgcccgg tgtcgtcgtc cttgaaaatg aagtattgga
     2461 cgtcgagcgt gcgctgcgcc cggtttgcca tctgcacgcg cagcgtgaac gcgtcgctgc
     2521 cgctggcgaa gagacggaat cccgacaatc cgggatgcgc cttcgccagt gtctcgacac
     2581 cctgcccgag cggcgtgctc gcaggatcga ccagcgcggt cgacgcaatc ttcggatagt
     2641 cggcccccgg cggcatcgat gcgcagccgg caagcagcag cgacagcgca acgatccacg
     2701 ccacgagtcc gtgtgcctgc aacgagcggt tcatcagcga gcgaattgac ggggaacggg
     2761 taggaaagca cgtatcacgc gcgcgtcgcg tccgcagcgt gctgattgtg ctatcggtcc
     2821 gcgacgcgag actgcgtcgg cgctgtccta cacgcattgc cccgcgcgcc tgacaacgct
     2881 gcgtgttgcg ggtcgatcag acggcgcgat gcgcgtgaag gctcacgcta taatgcgcga
     2941 ccgcggagag atggccgagt ggtcgaaggc gcgtgactcg aaatcacgta taccgcaagg
     3001 tatccagggt tcgaatccct gtctctccgc catatcaccg cgtgtacggt cccacgccga
     3061 taggcggcgg agcgaggctc acgatgcgcc ggaaaagcgc gtcgtattgc gcgcccgttt
     3121 cgacgttgaa cagcggatcg ctgtttccgg cgaatgcgtc gtcgatctgc ctccaatcgc
     3181 tgtcggtcag atgcgcgcgg gcgagcggca gcagccggtc ttcttccgcg cgcatgtgtt
     3241 cccagtggaa cgctgcgtag gcgcggaccg cttcggcaaa ggcagcaaac tcgctcttcc
     3301 cgccttcctc atagcggcgc agcgcctgct cgagcgcgcg gatcttctcc gcacccgcgc
     3361 gatgctccgt tgccagccgg tcgacaagcg ctgtggcgtc cgccgaccga aggcgcaaca
     3421 acttgaacag gtattcatct tccttcggat ggtggaagcg ctcggcgaac gcatcgatgt
     3481 agtaaaccat cgcaccgaac agctcgaaat tgggagcgct gttgcgcaac ccgatttcgc
     3541 ggacgagatg cagcatcccg tgcagcaccg ccgcgagcga ccggtgctcc gaatgaatga
     3601 tacgaatcgc gttcatggca cgccctcgac ggcgttgccg gttccgatga gccggcgtcg
     3661 ctgggaacat tagcgcacgc aaggacgatc gccattgatg caggtcaatc gatgtccggg
     3721 tgtggttttt gcacggcgta tccgcacgcc ttttttgcgg ggacggacgc cattcgtggg
     3781 taagaattac ccgcgcaggg aagaatcgtc tgcgcggtga ccgttttttt cgattgtgaa
     3841 gggcaccatg gaagtcccgg cagcagcgtt cgacatctcg cgcgagggtg caggcggcgc
     3901 ctcgccgcac gagcatttcg tgcagttcta cgaaaccgag ccggcgctcg tcgagtcgat
     3961 tgccgatttc gtcggcaccg gcctttcggc gggcgccgcc gcgatcgtga tcgccacgca
     4021 acctcacctg gcgcagctcg acgagcgctg ctcggcgcgc ggcatcgacc tcgcatctgc
     4081 gcgtggcgaa ggtcgctacg tcgtgcgcga tgcgcgtacg agcgtcgaat cgcttctcgt
     4141 ggaggggtgg cccgacgccg gtcgcttcac ggccaccctc gagccgctcg tcgcggccgc
     4201 cgcacgacgt ggctcgcggg tggtgatctt cggcgaaatg gtcgccctgc tgtggaacga
     4261 cgggcgccac gatgcggccg tgcaactcga gcaactatgg aacgagctcg cgcaaaggta
     4321 cgcgttttca cttttctgcg cctatccgat tgcgcagatg caaagcggcc cgtggccggc
     4381 gatgcacgcg gtctgcgcgg cgcattcgaa cacgatcccc acgcatgcga cgcggtacgc
     4441 agacgaggag aagcggcttt ccgagctgtg cgatctgcag cggcaggcgc gcgcactcga
     4501 gcacgagatc gggcaacggc gggctaccga gaacctcctc gcaagccgcg agcgcgagct
     4561 cgcggatatc ctcgagaatg ccgcgcaggc gatccacagc gtgggtcccg acggcgtgat
     4621 cctgtgggcg aaccagtgcg agctcgacat gctgggctat gcggccagcg aatatgtcgg
     4681 ccatccgatc gccgacttct atgtggacgc aagcgtggca gcccggattc tgcgccggct
     4741 tggcgccggc gaatcgcttc gtgacgagcc ggtgcagatg cggcacagga atggatccgt
     4801 ccgcgatgtg ctggtcacgt ccaacgttcg ctttgaacac ggcgcgttcg cccagacgcg
     4861 ttgcttttct cgcgacgtga ccgagcagcg atgcgtggaa cgcgcgcttc gcgaaagcga
     4921 ggagcacgcg cagcacacgc gctcgctgct ggcgtcgatc gtcgaatcgt ccgatgacgc
     4981 gatcatcagc aagtcgctcg acgggcggat cacgtcgtgg aacgcgagcg cgaggcgggt
     5041 ttttggctat acggccgagg aagcggtcgg caagccgatc acgatgatca tcccgcccga
     5101 gcatcagcac gaggagcggc tcatcctcga gcggctcggg cgcggggagc gcatcgagca
     5161 cttcgaaacg gtccgcgttg caaaggacgg ccggcgcatc gacgtttcgc tcaccatttc
     5221 gccggtgcgc gatcaacgcg gccgcgtcgt cggcgcatcg aaggtggcgc gtgacgtatc
     5281 cgagcgcaag cggatggaag agagattgcg cgacgccgat cggcgcaagg atgagttcat
     5341 cgcgatgctc ggccacgagt tgcgcaatcc gctcgcgccg atccgcaacg tggccgcgct
     5401 gctgcgcagg acaaccgcgg gggacagcgc atcggcggag ctttgcctga tgctcgagcg
     5461 gcaggtgcaa cagatgacgc ggcttctcga cgacctgctc gatgtgaacc gcatcacgcg
     5521 cggcaacatt cgtttccagc gcgagctgct cgatctcgcc accgtcgtgc aacacgcggt
     5581 cgaggcaagc ggtccgatca tcgaccgtcg cggtcaccgg ttgcacgtcg atttgccggc
     5641 agaaccgctg cgggtcaacg gcgatgccgc gcgactggtg cagatgctga ccaacctgct
     5701 caacaacgcg gcgaagtaca cgccggacgg tggacgcatc gagctcgcgg tggcccgccg
     5761 cgacgcgtcg ctcgagctgc gcgtgaagga caatggcgtg ggcatcgcac tcgagatgct
     5821 gccgagggtt ttcgacctgt tcgtccaggc agaccgctcg ggcggcgacg tgcaggaggg
     5881 gctcgggctc ggcctcacgc tggttcgcgt gatcgccgag catcacggcg gtgtcgtcga
     5941 ggcggtgagc gcgggccctg gacgcggaag cgaattcatc gtcaggctgc ccgacgccga
     6001 agcgtcgcgc ccggtcgatg cggctcgcgc gcaaggcaga cgcagcgtcg ccgtcgacgg
     6061 ccacgccgct cgcgacgcgt tgcggcctca tccattcggg tgattcgcgg gggcgcccgg
     6121 ccgcgctatc atcgccgcag ctgcgaataa tcgcagccac gggcgggctg gctgtccggt
     6181 atgctgcgcg cgtccccgct ggtgacaggt cacaggaacg cttcgatcga acggcgctgc
     6241 gcagcacact actccgcccg aatgtcagcg aacgtcatcg agctgcgcaa ggaagcccgc
     6301 cgccgcgtgc tggtcgcaca ccagcgcagc gacgtgcgcc acgcgctgcg cacgctgatc
     6361 gagcaggaca acgtgattgt tgtcgaggcg gccgagggtg atgcggcgct cgcggagctc
     6421 gaattcgggc ggttcgacct cctgctgctc gagctcgatc tgcccgtcaa ggacggcatt
     6481 gccgtcatgc agcttcatcg gatgctgctc gcgcacgagc acgtccacgc cgagccgccg
     6541 gccgtggtgc tcacgctgcc gcccgaagtg cgcgacaacg cagcgctcac cgatcacctg
     6601 cgcacgctcg gcgtgatcgc gttcatcgac gacgcaccga ggcccggcgt ggcgagcctc
     6661 gtcgacgaca tcctgcgggc gcgcgccgcc gaaagcgatt cccacaagcc tgccgttgcc
     6721 tgaaggggca tggcacaatc cggacgtctt gcgcgaagcc tcggagagtg cgatcacggc
     6781 cccggccagc agccgcttcg gcagcggcaa atcggtcaag cgcgtcgagg atgacgccct
     6841 tctgcgtggc ctgggccgct tcgccgacaa cctgcccgcg ccgggccagc ttcacacgta
     6901 tttcgtgcgt tcgccgcatc cgcacgcccg catcacgcgc atcggcagcg atgccgcgct
     6961 gaagctgccc ggcgttgccg cggtcttcac gggtcgtgat ctcgccggcg ccggcgtgaa
     7021 gccgattccc acctctgccg attatcggcg cgccgacggg tcgccgacgg caagcccgct
     7081 gcgccacgcg cttgccgtcg atacggtgcg cttcgttggc gaagccgtgg ttgccgtcgt
     7141 cgccgcgacg ctgcacgagg cgcgcgatgc ggccgaccgc gtcgaggtcg actacgagcc
     7201 gttgcctgcc atcgtcggcg tcgacgcggc aatcgccccc ggtgcgcctg ccgtcgttgc
     7261 cgaagcgccc gacaacgtgg cctgcgaagc gcgccacggc aatcggcgcg atgccgaagc
     7321 ggcgtttgcg cgcgccgcgc acgtcgtggc actcgacctc gtcaaccagc gtgtcgcgcc
     7381 gtcgccgatc gagccacgct cgacgctggc gatgtgcgac gccgccaccg gccgcctcgc
     7441 gctgcgggtg agctgccaga cgccgacggg gctgcgcgac gagctttgcg aagtcctcgg
     7501 catcgaaagc gacaaggtgc gcgtgctcgt cggcgatgtc ggcggcggct tcggcatgaa
     7561 gacgagcctg tatcccgagg atgtcgccgt cgcctttgcc gcgcgcaagc tcgggcgccc
     7621 cgtcaagttc accgccgagc gcatcgagga attcctcgcg gcaagccacg gacgcgacgt
     7681 cacgagccgg gccgagctcg cgctcgatgg caacggacgc attctcgcgt tgcgcatcgt
     7741 gtcgctcgcc aatgtcggcg cctacgcaac gccggccggc gtggtgatcc agctcctgat
     7801 cgggccgtgg gtgtcgacca gcgtctacga catccggacg atcgacatcg gcatcaaggc
     7861 gatcctcacg cacacgctgc caacgggacc ctatcgcggg gccgggcgtc ccgaagcgat
     7921 ctacatcatc gagcggctga tcgacgaggc ggcgcggcag acgggcatcg accgcgtcga
     7981 gctgcgccag cgcaacatga tcgaccccgc gcagatgccg tacacgaacg cgatggacaa
     8041 gacgtacgac agcggccagt tcgagaaggt gatgatgcag gcccttgcgc gctccgcatg
     8101 gaacgatttc agtgcgcgcg cggaacgttc gcgcgcagag ggacgctggc gggggcgagg
     8161 catcgcgacg tttctc
//
LOCUS       DASSVX010000012         5841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_750167, whole genome shotgun sequence.
ACCESSION   DASSVX010000012 DASSVX010000000
VERSION     DASSVX010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750167"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /locus_tag="VFO53_00610"
     CDS             <1..1303
                     /locus_tag="VFO53_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8874801.1"
                     /translation="GVLTLGLDGAARRFDADALAMVTDLATRGATALDNALLFRKIQD
                     EDQRKNEFLAMLAHELRNPLAPISNAVHILRVSEDDASKIAWARELIARQLKQLVRLV
                     DDLLDVSRITRGKIELKVEMVDVGQVVAAAIETSKPNIDAQRHALSLQLPSEPMQLVG
                     DFARVAQILSNVVNNAAKYTPKGGRISLSAARESDEIVFRVRDSGVGIPPEFLTSIFD
                     PFTQVDRTLARSHGGLGIGLTLVRRLVEMQNGRVSVRSEGRNRGSEFTVRLPAADAAT
                     APASEVIEARATQPSPAGLRVLVVDDNRDVADSTASIMRMNGCDVHVAYDGKAALESV
                     QRLRPDAILLDIGLPAIDGYLVAEHIRAQPENGRTMIVAVSGYGQEQDRLRSKSVGFD
                     YHVVKPIDPNVLAGLVGSLRLSRDAAQGDIAARAPLSSAAG"
     gene            1306..2898
                     /locus_tag="VFO53_00615"
     CDS             1306..2898
                     /locus_tag="VFO53_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET8874802.1"
                     /translation="MSTQATRASGIGPAGLADLAERAFARAAGSPSTEGNAVRLLCDA
                     RENFPAWRDALAAARRYILFESYIVENDRVGRDFATLLADKARAGVRVHVIVDWLGCW
                     RALSLWRPVRDAGADVRVFNPPRISSPLGWLSRDHRKTIVVDGEVGFVSGLCVSERWL
                     GDPKRRLEPWRDTGIEIRGPAIAALEHAFVRVFAASGAPLDTQGFAEAESIAPAGSMR
                     LHVIANEPNLAGTFRMDLVIASIARRQLWLTDAYFVATSPYVQALRAAARDGVDVRLL
                     VPGSSDIPALSPLSRAQYRPLLEAGVRVFEWAGTMLHAKTAVADRRWSRVGSTNLNLA
                     SWMSNYELDVAIEDASFSEQMATQYETDLGHATEIVLTRRNRVRRAPQGVQEPGPHAR
                     TRRAASGSAGRAAAGAFSVGSALGAALTDRRILGPAEAGLLFLMAAVAAVIGVVGALW
                     PRVLAWPVAFVALWSGTAWIVKGISLRRGRRGGPRVERLEDALPDTGAPPAEPSPQPP
                     QSVQTEAERAQTGAERDRITSR"
     gene            2969..4114
                     /locus_tag="VFO53_00620"
     CDS             2969..4114
                     /locus_tag="VFO53_00620"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET8874803.1"
                     /translation="MRESASVQRAGVRTTPPFRADHVGSFLRPKFLLDAREQFRTGAI
                     DAARLRSVEDEAIRGVVKFEEDLGLHGITDGEYRRTYFHIDFLTQLQGVETKGGIHVK
                     FHSAKGDVDFEPPVMQVTGEVRHEKPIQRADFEFLRSVTSRTPKVTIPSPTMLHFRGG
                     RNAISDKAYPDLDRFYADVAAAYADELQSLGDAGCTYVQFDDTNLAYLCDETMREGAR
                     KRGDDPNQLPRRYARLINAAIERRPKGMTACVHLCRGNFRSAWAAEGGYEPVAEVLFN
                     ELEVDGYFLEYDDPRSGDFAPLRHVPKGKTVVLGLVSTKVGALESKDTLKRRIDEAAK
                     FVPMEQLCLSPQCGFSSTVHGNDIAVESQAAKLRLVIETARDVWGAV"
     gene            complement(4202..5044)
                     /locus_tag="VFO53_00625"
     CDS             complement(4202..5044)
                     /locus_tag="VFO53_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET8874804.1"
                     /translation="MRLLIGLAVAALALTGGAAGLVYSGVYDIAATDQHTAPVFWLLK
                     TAMRRGVVRHARDIEVPPLGDPVQVAVGRALYEANCVRCHGAPGVAPERFAFGLRPLP
                     ANLANTGIEWPAPRLYWTIKYGLKLTGMPAWEFRLRDDELWAIVAYVQRLPYESPREY
                     RELRARQRPATPGEGDEPARSNGLQAMTGNPTRGRHVMQQYRCVACHEIPGVVGASVP
                     VGPPLDRMGARSFIGGVLENTPKNMVRWLLAPQRFVPDGAMPDLGVPDGDARDMAAYL
                     DTLR"
     gene            complement(5054..5470)
                     /locus_tag="VFO53_00630"
     CDS             complement(5054..5470)
                     /locus_tag="VFO53_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET8874805.1"
                     /translation="MNAFCERRQAPASKAHRARHTKPLVALSMVAALAACSDPSLHPQ
                     AAGDATRGRMLLQQYGCGACHRIPGVPGANSAIGPSLDRLGRRVYVAGVLPNSPAELA
                     RWIRAPDVAKPGTAMPNANVTERDATDIVAYLYQLR"
     gene            5651..5727
                     /locus_tag="VFO53_00635"
     tRNA            5651..5727
                     /locus_tag="VFO53_00635"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5685..5687,aa:Arg,seq:acg)
BASE COUNT          948 a         1934 c         2021 g          938 t
ORIGIN      
        1 cggcgtgctc acgctcgggc tcgacggcgc ggcccgccgc ttcgacgccg acgcgctcgc
       61 catggtgacc gacctcgcca cgcgcggcgc aaccgcgctc gacaacgcgc tgctcttccg
      121 caagatccag gacgaggacc aacgcaagaa cgagttcctc gcaatgctgg cgcatgagct
      181 gcgcaatccg ctcgcgccga tcagcaacgc ggtgcatatc ctgcgcgtca gcgaggacga
      241 tgcatcgaag attgcgtggg cacgcgagct catcgcccgc cagctcaagc aactggtgcg
      301 cctcgtcgac gatctgctcg acgtgtcgcg catcacgcgc ggcaagatcg agctcaaggt
      361 cgagatggtc gacgtgggac aggtcgtcgc cgcggccatc gagacgagca agcccaacat
      421 cgacgcgcaa cgccatgcgc tgtcgctgca gcttccgagc gagccgatgc aactcgtggg
      481 cgatttcgcg cgcgttgcgc agatcctgtc gaacgtggtg aacaatgccg cgaagtacac
      541 gccgaagggc gggcgcatct cgctgtcggc cgcccgcgaa agcgacgaga tcgtgttccg
      601 cgtccgcgac tcgggtgtcg gcatcccgcc cgagttcctg accagcatct tcgatccctt
      661 cacgcaggtc gaccgcacgc tcgcacgctc gcacggcggg cttggcatcg gccttacgct
      721 ggtgcgccgt ctcgtcgaaa tgcagaacgg ccgcgtgagc gtgcgcagcg aaggccgcaa
      781 ccgcggcagc gagttcaccg tccggctgcc ggccgccgac gcggcaacgg cgccagccag
      841 cgaagtgatc gaagcgcgcg ccacgcaacc gtcgcccgcg ggcctgcgcg tactcgtcgt
      901 cgacgacaat cgcgatgtcg cggacagcac ggccagcatc atgcgcatga acggttgcga
      961 cgtgcatgtc gcctacgacg gcaaggcggc gctcgagtcg gtgcagcggc tgcgtcccga
     1021 cgcaatcctg ctcgacatcg gtctgccggc gatcgacggc tatctcgtcg ccgagcacat
     1081 tcgcgcgcaa cccgagaacg ggcgcacgat gatcgtggcg gtgtccggat atggccagga
     1141 gcaggaccgg ctgcgctcga agagcgtcgg cttcgactac cacgtcgtga agccgatcga
     1201 cccgaacgtg ctcgcgggac tggtgggctc gctgcggttg tcgcgcgatg cggcgcaggg
     1261 cgacatcgcc gcgcgtgccc cgctgtcgag cgcggccggc tgacgttgtc gacgcaagcg
     1321 acgcgcgctt ccggaatcgg ccctgccggt ctcgccgacc ttgccgagcg cgcattcgcg
     1381 cgcgccgcgg gctcgccgtc caccgagggc aacgccgttc gcctgctgtg cgacgcgcgc
     1441 gaaaactttc ccgcgtggcg cgatgcgctc gcggccgcgc gccgctacat cctgttcgaa
     1501 tcgtacatcg tcgagaacga tcgcgtcggt cgcgatttcg cgacgcttct cgccgacaag
     1561 gcgcgcgccg gcgtgcgcgt gcatgtgatc gtcgactggc tcgggtgctg gcgggcgctg
     1621 tcgctgtggc ggcccgtccg cgatgccggg gccgacgtgc gcgtgttcaa cccgccccgg
     1681 atttcgagcc ccctcggatg gctgtcgcgg gaccaccgca agacgatcgt cgtggatggc
     1741 gaagtgggat tcgtcagcgg gctgtgcgtc agcgagcgct ggctcggcga cccgaagcgc
     1801 aggctcgagc catggcgcga taccggcatc gagatacgag gcccggcgat cgccgcgctc
     1861 gagcatgcgt tcgtccgcgt gttcgcggcg tccggcgcgc cgctcgacac gcaagggttc
     1921 gcggaagcag aaagcatcgc gccggcaggc tcgatgcggc tgcacgtgat cgccaacgag
     1981 ccgaacctcg ccggcacgtt ccgcatggac ctcgtgattg cgtcgatcgc gcgcaggcaa
     2041 ctgtggctga ccgatgccta tttcgtggcg acgtcaccgt acgtgcaggc gcttcgcgcg
     2101 gcggcgcgcg acggcgtcga cgtgcgcctt ctcgtgccgg gctcgagcga cattcccgcg
     2161 ctgtcgccgc tgtcgcgcgc gcaataccgg ccgttgctcg aggcgggtgt gcgcgtgttc
     2221 gaatgggcag gcacgatgct gcacgcgaag acggcggtcg ccgatcggcg ctggtcgcgc
     2281 gtcggctcga ccaatctcaa tctcgcgagc tggatgtcga actacgagct cgacgtggcg
     2341 atcgaggatg catcgttctc cgagcagatg gcaacccagt atgaaaccga tctcggccat
     2401 gcgaccgaga tcgtgcttac ccggcgcaac cgcgtgcggc gggcgccgca gggcgtgcag
     2461 gagccgggtc cgcatgcacg cacgcggcgt gctgcgtcgg gaagcgcagg acgcgcggca
     2521 gcgggtgcgt tcagcgtcgg gagcgccctc ggcgcggcac tcaccgatcg gcgcattctc
     2581 gggccggccg aggcgggact tttgttcctg atggcggccg ttgctgcggt catcggcgtc
     2641 gtcggcgcat tgtggccgcg cgtgctcgca tggccggtcg cgttcgtcgc gttgtggtcg
     2701 ggcaccgcgt ggatcgtgaa gggcatttcg ttgcgacggg ggcgccgagg cggcccgcgc
     2761 gtcgagcggc tcgaggacgc gctcccggat accggcgcgc cgccggcgga gccgtcgccg
     2821 cagccaccgc agagcgtgca gaccgaagcc gagcgcgcgc agaccggggc cgaacgcgat
     2881 cgcattacgt cgcgatgacg cgtggccgcg ctgcggcgag cgacgagtgc tacgctttcg
     2941 tgcgcaaggt gactgatcag gagcaaccat gagggaatcc gcaagcgtgc agcgtgccgg
     3001 tgtccggacg acgccgccgt ttcgtgccga tcacgtcgga agtttcctgc gtccgaagtt
     3061 tctgctcgac gcgcgcgagc agttcagaac aggcgccatc gacgctgcaa ggctgcgcag
     3121 cgtcgaagac gaagcgatcc gcggcgtcgt caagtttgag gaggacctcg gcttgcacgg
     3181 gatcaccgac ggcgaatatc ggcgcaccta cttccacatc gacttcctca cgcaactgca
     3241 gggcgtcgag acgaagggcg gcattcatgt caagttccac agcgcgaagg gcgacgtcga
     3301 cttcgagccg ccggtgatgc aggtcaccgg cgaggttcgc cacgaaaagc ccatccagcg
     3361 cgccgatttc gagttcctgc gctccgttac gtcgcgcacg ccgaaggtga ccattccctc
     3421 gccgacgatg ctgcattttc gcggcgggcg caacgccatc agcgataaag cgtatcccga
     3481 cctcgaccgc ttctacgccg acgtggcggc tgcctacgcc gacgagttgc aaagcctcgg
     3541 cgacgcagga tgcacgtacg tgcagttcga cgacacgaac ctcgcgtacc tgtgcgacga
     3601 gacgatgcgc gaaggcgcgc gcaagcgtgg cgacgatccg aaccagttgc cgcgtcgcta
     3661 cgcgcgattg atcaacgcgg cgatcgagcg ccggccgaag ggcatgaccg cgtgcgtgca
     3721 tttgtgccgc gggaatttca ggagcgcgtg ggcggcggag ggcgggtacg agcccgtggc
     3781 ggaggtgctt ttcaacgagc tggaagtcga cggctacttc ctcgagtacg acgacccgcg
     3841 ctcgggcgac ttcgcgccgt tgcgccatgt gccgaagggc aagaccgtcg tgctcgggct
     3901 cgtcagcacg aaggtcggcg cgcttgaatc gaaggacacg ctgaagcggc gcatcgacga
     3961 agcggcgaaa ttcgtaccga tggaacagct gtgcctgtcg ccgcaatgcg ggttctcgag
     4021 caccgtgcac ggcaacgaca tcgcggtcga atcgcaggcg gcgaagctgc ggctggtgat
     4081 cgagactgcg cgcgacgtgt ggggcgccgt gtagtcgcgc gcggcttgcg aaggcgcgcg
     4141 tcgcgctgcc cgcgccggtc tccgctgccc atgcgagctt cgattgcgcg gctgaatccg
     4201 ttcagcgcaa cgtgtcgagg tacgcggcca tgtcgcgggc gtctccatcc ggcacgccaa
     4261 gatcgggcat ggcgccatcc ggcacgaagc gctgcggggc gagcagccag cgcaccatgt
     4321 tcttcggcgt gttctccagc acaccgccga tgaagctgcg cgctcccatc cggtcgagcg
     4381 gcggacccac cgggacactg gcgccgacga cgccgggaat ttcgtgacac gcaacgcagc
     4441 gatactgctg catcacgtgg cgtccgcggg tgggattgcc cgtcatcgcc tgcaacccgt
     4501 tgctgcgcgc aggctcatcg ccttcgcccg gcgtcgctgg tcgctggcgc gcacgcagtt
     4561 cgcggtactc ccgcggcgac tcgtacggca gccgttgcac gtaggcgacg attgcccata
     4621 gctcgtcgtc gcgcaatcga aactcccagg cgggcatccc ggtcagcttg agcccgtact
     4681 tgatggtcca atacagccgc ggcgcgggcc attcgatgcc tgtgttggcg aggttcgcag
     4741 gcaggggccg caatccgaac gcaaatcgct ccggcgcgac gccgggcgcg ccgtgacatc
     4801 ggacgcaatt cgcctcgtag agcgcacggc cgacggcgac ttgtacagga tcgccaagcg
     4861 ggggtacctc gatatcgcgc gcatggcgca cgacgccacg gcgcatcgcc gtcttcaaca
     4921 gccagaagac gggtgccgtg tgttgatcgg ttgccgcgat gtcgtagacg cccgaataga
     4981 cgaggcccgc ggcgccgccg gtcaatgcga gtgcggcgac ggcgaggccg atgagaaggc
     5041 gcatcggatc gcttcagcgc aactgataga gatatgcgac gatgtccgtc gcatcccgct
     5101 cggtgacatt cgcgttcggc atcgcagtgc caggctttgc aacgtcgggg gcgcgtatcc
     5161 agcgcgcgag ctccgcggga gaattgggca gcacgccggc aacgtacacg cgccgcccca
     5221 gccgatcgag agacggaccg atcgctgagt tcgcgccggg cacgccggga atgcggtggc
     5281 aggcgccgca gccgtattgt tgcaacagca tgcgaccgcg cgtcgcgtcg cccgccgcct
     5341 gcggatggag cgatggatca ctgcaggcgg caagggctgc gaccatcgac agcgcgacga
     5401 gcggtttcgt gtgacgcgcc cggtgggctt tgctcgcggg cgcttgtcgt cgttcgcaga
     5461 aagcgttcat cgacggcatc cttgttcctt gtcaagggca cggtacgccc gaacgagctt
     5521 catttaccgt ggtcctgccg ggcttccaat ttactcctga aagccggtga gactacgcat
     5581 cgcggaatcg gcgtgggcaa gggggacgcg cggccgtccc cggatctcga cgtgttagaa
     5641 ttcgggcttt gcgcccgtag ctcagttgga tagagtacct ggctacgaac cagggggtcg
     5701 tgggttcgaa tcctgccggg cgcgccaaat tttccagggc ttgcgatgat cgcaggccct
     5761 tgccatttgg tgcctgtagc cgaaaacgta gccacttccc cgtctttcac gagccgcaag
     5821 cccgacgtgc ggtcgacgta c
//
LOCUS       DASSVX010000013         7124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1183542, whole genome shotgun sequence.
ACCESSION   DASSVX010000013 DASSVX010000000
VERSION     DASSVX010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183542"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /locus_tag="VFO53_00640"
     CDS             complement(<1..1381)
                     /locus_tag="VFO53_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HET8874806.1"
                     /translation="MANIDSLLHGFGVALSGYHVLLMIVGVLLGILVGVLPGLGAPNG
                     VTLLLPLTFGMNPVAAIILLTSMYWGALFGGSTTSILFNIPGEPSSVATTFDGYPMAR
                     NGQPAQALTLAFLSAAFGALVGVILITVLSTWVANFALRFGSPEYFAVYFLAFASFVG
                     LGGAAPLKTIVSMGLGLALATIGMDTISGSLRLTFGFDTLVSGVSFLVAVIGLFGIGE
                     LLISMEEGLRFDGVPARISPRAVLETIRSMPRHWVALLRGSAIGCWMGITPGGPTAAS
                     FMSYGLAKRFSRRGRYFGSGEPEGVISPETADHAAGTSAMLPMLALGVPGSATSAVML
                     GGLMIWGLNPGPTLFVDRPDFVWGLIASMYVANIVAVVLVLSTVPLFAAILRIPFAII
                     GPMIVVVCFIGAYTVAGSKPDLLFAVLFGIAGYAFKKLDYPIAPLVLAMVLGDKAEDA
                     FRQSMIMSKG"
     gene            complement(1510..1983)
                     /locus_tag="VFO53_00645"
     CDS             complement(1510..1983)
                     /locus_tag="VFO53_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HET8874807.1"
                     /translation="MTRFAAELVVALVTLALGAAVIVGAREYGTGWNASGPEPGAFPF
                     YVGLIVVAASAGNLFVAWRRRDGHRVFVEADQLRRVAAFGVPLLGFVALSLTLGFYVA
                     TAIYLGGVMRVQGRYRWLSCIAVAFGTAAFFYLVLEVWFKVPLLKGPLEALLGVH"
     gene            complement(2003..3016)
                     /locus_tag="VFO53_00650"
     CDS             complement(2003..3016)
                     /locus_tag="VFO53_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET8874808.1"
                     /translation="MSNTRKMRKVAAVVMGAVSLSLIATGAAADWKPSQPIEFIVTAG
                     PGGGTDNFARTIQAIVTKYKLIDQPIVVVNKGGGSGAEGFQYGKSPADDAYKVTFATN
                     NEYLLPLIAKLSWKGEDFTPVAAMAVDEFILWVNGKSEFKTAKAYIDAAKAKPGTFKM
                     GGSQSKDTDQTLTSEITAATGATFLYVPFKSGGEAAVQLAGGHIDSNTDNPSESIGQW
                     KGGLVMPLCVFSAKRLAPGPKVTDTMGWSDIPTCAESGIPIEQYQQPRTVWVPKGAPA
                     DAVAYYAGVLAKVRETPEWKTYIERSAQTDRFLTGAEFKRFAVTDEQKARKVFEQEGW
                     LVR"
     gene            complement(3054..4034)
                     /locus_tag="VFO53_00655"
     CDS             complement(3054..4034)
                     /locus_tag="VFO53_00655"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET8874809.1"
                     /translation="MGDPAGISPELTARAMALPEIRDAAELIVIGDARVFAAGATTAQ
                     VASDVEFRNGLDDRPSSGRPIFLDLAHLDPSTVTPSEASAAGGEFALANFRTALQLAA
                     NGRVDAVCFTPFNKAAMRAAHPGYDDEIAYSAEVLGTSGPASEFNILEGLWNARVTSH
                     IPLSAVAASITRQRVVDAIRLTDHALREANVAAPRIAVAALNPHAGDAGNFGREEIDV
                     IAPAIEDVQREGIHADGPFPSDTVFVRARNGNFDAVVTMYHDQGQIAMKLMGFDRGVT
                     LMGGFPFPIVTPAHGTAYDIAGKGIANVGATRAALLLGARMAARRIDARA"
     gene            4201..4884
                     /locus_tag="VFO53_00660"
     CDS             4201..4884
                     /locus_tag="VFO53_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8874810.1"
                     /translation="MSTIMPGAAPTPAIVPLSRPTLHDQLIDRVRALIVEGQLAPGTR
                     IHEGELGKALGVSRTPLREALKVIASEGLVELVPGRGAVVRKLTVKDVAEMLDVLKAL
                     ETLAARLACRNATDLQIASLRRMHDEMIAFYESRNRLDYYWRNQAIHAGLAELSGNAL
                     LATMHATIQARMKRIRFIGNEEPALWAAAVAEHEEMIAALEARDEARLAGVVARHLDE
                     TWKRVADKL"
     gene            complement(4923..5612)
                     /locus_tag="VFO53_00665"
     CDS             complement(4923..5612)
                     /locus_tag="VFO53_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HET8874811.1"
                     /translation="MLVHPCLPEAVFPARRIEWRGHVSREGPPQATAPPSGAASSASV
                     GGRQQRRHYNRAMEPLLPAFRRELRARAHHLRPVVSIGHHGLTPAVLHETDVALTAHE
                     LIKVRVFADDRDERGSLLKALCTALDCAGVQQIGKLLVLWRPRPDEPQDARDSPRTKP
                     AMRKNGPAAKNAPMRKSAFVAKEAAATRRRTAGPVAQPEPVVPRRRAPAGKAPPGVPR
                     AAAPRRRRVPR"
     gene            5601..6197
                     /locus_tag="VFO53_00670"
     CDS             5601..6197
                     /locus_tag="VFO53_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HET8874812.1"
                     /translation="MHEHLTDPYVREANRLGYRSRAAFKLTELAERDRLFRPGMTVVD
                     LGSAPGSWSQVLREALGARSRIVAVDLLPMDGVAGVQFVQADFESDEGLAAVESALRG
                     APVDLVVCDMSPNLSGIDVVDQARSIGLAELALDFARAHLQPGGDLVVKVFQGAGLDA
                     FQRAVRSHFTKTYLRKPKASRDRSREHYLVAKGLRAPA"
     gene            6393..>7124
                     /locus_tag="VFO53_00675"
     CDS             6393..>7124
                     /locus_tag="VFO53_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HET8874813.1"
                     /translation="MNNLLKNIGIWLVIGLVVLTVVKQFDSRQTSKDTVAYSDFMEQA
                     RSGRVESASVEGRTIKWVSTDKKRYVTYSPGDIWMVGDLVKDGVKVEARPEEEQSFLA
                     QIFISWFPMLLLIGVWIFFMRQMQGGGRGGAFSFGKSKARMLDESNNTVTFADVAGCD
                     EAKEEVGELVEFLRDPSKFQKLGGRIPRGVLMVGSPGTGKTLLAKAIAGEAKVPFFSI
                     SGSDFVEMFVGVGAARVRDMFEQAKK"
BASE COUNT         1206 a         2359 c         2422 g         1137 t
ORIGIN      
        1 atcccttcga catgatcatc gactggcgga acgcgtcctc ggccttgtcg ccgagcacca
       61 tcgcaagaac gagcggggcg atcggataat cgagcttctt gaacgcgtag cccgcgatgc
      121 cgaagagcac cgcgaagagg aggtcgggct tgctgcccgc caccgtgtac gcgccgatga
      181 agcatacgac gacgatcatc ggaccgatga tcgcgaacgg aatgcgcagg atcgccgcga
      241 acagcggcac cgtcgacagc accagcacga cggcgacgat gttcgcgaca tacatgcttg
      301 cgatcaggcc ccagacgaaa tcgggccggt cgacgaacag cgtcggtccg ggattgagcc
      361 cccagatcat cagcccgccg agcatcaccg ccgacgtcgc cgagccgggc acgccgaggg
      421 ccagcatcgg cagcatcgcg ctcgtgcccg cggcgtggtc tgcggtttcc ggcgagatga
      481 cgccctcggg ctcgccgctg ccgaagtagc ggccgcgtcg cgaaaagcgc ttcgcgagcc
      541 cgtagctcat gaacgacgcg gcggtcggtc cgccgggggt aattcccatc cagcagccga
      601 tggcgctgcc gcgcagcagc gcgacccaat gccgcggcat cgagcggatc gtctcgagca
      661 cggcgcgcgg actgatgcgc gcgggcacgc cgtcgaagcg cagcccttcc tccatgctga
      721 tcagaagctc gccgatgccg aagaggccga tcaccgcgac gaggaagctc acgccggaca
      781 cgagcgtgtc gaatccgaac gtgagccgca ggcttcccga gatcgtgtcc atgccgatcg
      841 tcgcgagcgc gaggccgagc cccatcgaca cgatcgtttt cagcggcgct gcgccgccaa
      901 gccccacgaa gctcgcgaat gccaggaaat agacggcgaa gtactcgggt gagccgaagc
      961 gcagcgcgaa attggccacc cacgtcgaca gcaccgtgat caggatcacg ccgaccagcg
     1021 ctccgaacgc cgccgacagg aacgcaagcg tcagcgcctg tgcgggctgg ccgttgcgcg
     1081 ccatgggata gccgtcgaag gtcgtcgcca ccgacgacgg ctcgccggga atgttgaaga
     1141 ggatcgacgt cgtcgagccg ccgaacagcg cgccccagta catgctggtg agcagaatga
     1201 tcgccgcgac cggattcatg ccgaacgtga gcggaagcag cagcgtgacg ccgttcggtg
     1261 cgccgagacc gggcagcacg ccgacgagga tgccgagcag cacgccgacg atcatcagca
     1321 gcacgtggta gccggacagc gcgacgccga agccgtgcag cagggagtcg atgttcgcca
     1381 ttgcgtcagt cgggaagggc cgccccgagc ggggcagcga gtgcgtgagc ccgacgggcc
     1441 gccccgaggg cgaacacccg agtgcgaagc acgaggatca tccaccttgg cgtgggggga
     1501 tgtttcatct cagtgcacgc cgaggagcgc ttcgagcgga cccttcaaga gcgggacctt
     1561 gaaccagacc tcgagcacca gatagaagaa cgctgcggtg ccgaaggcga ccgcgatgca
     1621 ggagagccac cggtagcggc cctgaacgcg catgacgcca ccgagataaa tggccgtcgc
     1681 gacgtagaag ccgagggtca gcgaaagcgc cacgaagccg agcagcggaa cgccgaatgc
     1741 ggcgacgcgg cgcagctgat cggcctcgac gaacacgcga tgcccgtcgc gccttcgcca
     1801 tgcaacgaac agattgccgg cactcgccgc aacgacgatg agacccacgt agaacgggaa
     1861 agcgcccggt tcgggcccgc tcgcgttcca tccggtcccg tattcgcgcg ccccgacgat
     1921 cacggcggcg ccgagtgcga gggtgacgag tgctacgacg agctcggcgg cgaagcgcgt
     1981 cacgagcgcc gtcgctcgca aatcagcgaa cgagccagcc ttcctgctcg aacaccttgc
     2041 gcgccttctg ttcgtcggtt acggcgaagc gcttgaattc ggcgccggtc aggaagcgat
     2101 cggtctgcgc cgagcgctcg atgtacgtct tccactcggg ggtctcgcgc accttggcaa
     2161 gcacgccggc atagtacgcc acggcatccg cgggcgcgcc cttcggcacc cagaccgttc
     2221 gcggctgctg atactgctcg atcggaatgc ccgactcggc gcacgtcggt atgtccgacc
     2281 atcccatcgt gtcggtgacc ttcggtccgg gtgcgagccg cttcgcgctg aacacgcaga
     2341 ggggcatcac gagcccgccc ttccactgcc cgatgctttc gctcgggttg tcggtattcg
     2401 aatcgatgtg tccgccggcg agttgcacgg ccgcttcgcc gccgctcttg aacggcacgt
     2461 agaggaatgt cgcgccggtt gcggcggtga tctcgctcgt gagcgtctga tcggtgtcct
     2521 tcgactggct gccgcccatc ttgaacgtgc cgggtttcgc cttcgcggcg tcgatgtacg
     2581 ccttcgccgt cttgaactcg gatttgccgt tcacccacag gatgaactcg tcgacggcca
     2641 tcgccgccac cggcgtgaag tcctcgccct tccacgacag cttcgcgatc agcgggagca
     2701 ggtattcgtt gttcgtcgcg aacgtcacct tgtacgcatc gtcggcgggc gacttgccgt
     2761 actggaagcc ttccgcgccg ctgccgcccc ccttgttgac gacgacgatc ggctggtcga
     2821 tcagcttgta cttcgtcacg atcgcctgga tcgtgcgcgc gaagttgtcg gtgccgccgc
     2881 cgggacccgc ggtgacgatg aattcgatcg gctgcgatgg cttccagtcg gctgccgcac
     2941 cggtggcgat cagcgacagc gacactgcgc ccatgacgac ggccgcgacc ttgcgcatct
     3001 tgcgggtgtt cgacattttc ttcctcctcg tttaggcgat gcaatgcggg cggtcacgct
     3061 cgcgcgtcga tgcgccgagc ggccatgcgc gcaccgagca gcagcgcggc gcgcgtcgcg
     3121 ccgacgttgg cgattccctt gccggcgatg tcgtacgcgg tgccgtgcgc gggtgtgacg
     3181 atgggaaacg gaaagccgcc catcagcgtg acgccgcgat cgaagcccat gagcttcatc
     3241 gcgatctgtc cctggtcgtg atacatcgtc acgacggcgt cgaaattgcc gttgcgcgcg
     3301 cggacgaaca ccgtatcgga cgggaacggc ccgtcggcgt ggatgccttc gcgctgcacg
     3361 tcctcgatcg cgggcgcgat cacgtcgatt tcctcgcggc cgaaattccc ggcgtcgcct
     3421 gcgtgcggat tgagcgcggc gaccgcgatc cgcggagcgg cgacgttcgc ctcgcgcagc
     3481 gcgtggtcgg tcaggcggat ggcgtcgacg acgcgctgcc tggtgatcga cgcggcgacg
     3541 gccgacaacg gaatatgcga ggtgacgcgc gcgttccaca gcccttcgag gatgttgaat
     3601 tcgctcgccg gtccgctcgt gcccagcact tcggccgagt aggcgatctc gtcgtcgtag
     3661 ccgggatgcg cggcacgcat cgccgccttg ttgaacggcg tgaagcacac ggcgtcgacg
     3721 cgtccgttcg ccgcgagctg cagcgcggtg cggaagttcg cgagagcgaa ctcgcctccg
     3781 gcggcgctcg cctcggacgg cgtgacggtc gacggatcga ggtgcgcgag atcgaggaaa
     3841 atcggccgtc ccgagctcgg ccggtcatcg aggccgttcc gaaactcgac gtcggacgcg
     3901 acttgcgccg tcgtcgcccc tgccgcgaat acccgggcat cgccgatgac gatgagctcg
     3961 gccgcatcgc gtatttcggg cagtgccatc gcgcgcgcgg tgagctccgg actgatgccg
     4021 gccgggtctc ccatcgcgag ggcgatgagc ggacggccgc tgttgcggga agaaggcgac
     4081 acgtgggtac cggtagcggg caaatacgcg tatgctggat gctgcatgca gcatggaggg
     4141 atgtgacttt gacagcatcg acccacaccg tcaagccgtg atgcggcgag caagccagcg
     4201 atgagcacga tcatgccggg cgccgcgccc acgccggcca tcgtgccgct ttcccgcccc
     4261 acgctccacg atcagttgat cgatcgcgtg cgcgcgctga tcgtcgaagg ccagctcgct
     4321 cccggcacgc ggatccacga gggcgagctc ggcaaggcgc tcggcgtgtc gcgcacgccg
     4381 cttcgtgaag cgctcaaggt gatcgccagc gaagggctcg tcgagctcgt gccgggccgc
     4441 ggtgccgtcg tgcgcaagct cacggtgaag gacgtggccg agatgctcga cgtgctgaag
     4501 gcgctcgaga cgctggcggc gcggctcgca tgccgcaacg cgaccgacct tcagattgcg
     4561 tcgctacgcc ggatgcacga cgagatgatc gcgttctacg agtcgcgcaa ccggctcgac
     4621 tattactggc gcaaccaggc gatccacgcg ggtctcgccg agctttccgg aaacgcgctt
     4681 ctggcgacga tgcacgcgac gatccaggcg cggatgaagc gcatccgttt catcggcaac
     4741 gaggagcccg cgctgtgggc cgccgcggtg gcagagcacg aagagatgat cgcggcgctc
     4801 gaagcgcgcg acgaagcccg gctggcgggg gtggtcgcac gtcatctcga cgagacctgg
     4861 aagcgcgtcg cggacaagct ctgaaaccgt ccgtcggccg ccaccgcgga ccggcggagg
     4921 gttcagcgcg gcacgcgcct gcgcctcggc gccgctgcac ggggcacgcc gggaggcgcc
     4981 tttccggccg gcgcgcgccg gcgtggaacg accggttcgg gctgcgcgac gggtcccgca
     5041 gtgcgacggc gcgtcgctgc cgcttccttc gcaacgaacg cgctcttccg catcggcgcg
     5101 ttcttcgctg cgggcccgtt cttacgcatc gccggcttcg tccgcggcga atccctcgcg
     5161 tcttgcggct cgtccggtcg cgggcgccac aggacgagca gttttccgat ctgctgcaca
     5221 ccggcgcaat ccagcgcggt gcagagcgct ttgagcagcg atccgcgttc gtcgcgatcg
     5281 tcggcgaata cgcgcacctt gatgagctcg tgcgcggtca gcgcgacatc ggtctcgtgc
     5341 agcacggccg gcgtgagtcc gtggtgcccg atcgacacca ccggacgcag gtgatgcgca
     5401 cgggcgcgca gttcgcggcg gaatgccggc agcagcggct ccatcgctcg attatagtgg
     5461 cggcgctgct gacgaccccc tacgctcgcg ctgctcgctg cccccgaagg gggcgcagtg
     5521 gcttggggcg gcccttcccg actgacatga cctcgccact caatccgtcg cgccggaaac
     5581 accgcttcgg gaaggcatgg atgcacgagc atctgaccga cccgtacgtg cgcgaagcca
     5641 accgcctcgg ctatcgctcg cgcgccgcgt tcaagctgac ggagctcgcc gagcgcgacc
     5701 ggctgtttcg tcccggcatg acggtcgtcg acctcggctc tgccccgggc agctggtcgc
     5761 aggtgctgcg ggaagccctc ggcgcaaggt cccggatcgt ggcggtcgac ctgctgccga
     5821 tggacggcgt cgccggcgtg caattcgtcc aggcggactt cgaaagcgac gaagggctcg
     5881 cggcggtcga atcggcgctg cgcggtgcgc cggtcgacct tgtggtctgc gacatgtcac
     5941 ccaacctttc cggcatcgac gtcgtcgacc aggcacgcag catcgggctc gcggagcttg
     6001 cattggattt cgcgcgcgcc catctgcaac ccgggggcga tctggtggtc aaggtattcc
     6061 agggagcggg cctcgatgcg ttccagcgag cggtccgaag tcatttcacc aagacttatc
     6121 tacgcaagcc gaaggcgtca cgcgatcgca gccgcgaaca ttatctggtg gccaaggggc
     6181 tgcgcgcgcc ggcctgagtt gtcgctttcg ggagacaagt cgcgaaacgt tgcgtcgcga
     6241 caaacttcgc tacgctttcg ccgacgaagc gccggcggta accgcctttt caacggtaaa
     6301 atgacgcctg accttcgctt gccaccgggc gtgcgaggcg tgcgcccggc aggcagccgc
     6361 ccgggtcgat cgacggctgc aaggagcatt gcttgaacaa tttgctgaag aacatcggaa
     6421 tctggctcgt catcgggctc gtcgtgctca cggtcgtcaa gcagttcgac tcgcggcaga
     6481 cgtccaagga cacggtcgcg tattccgact tcatggagca ggcgcgcagc ggccgggtcg
     6541 aatcggcgtc ggtcgagggg cgcaccatca agtgggtgtc caccgacaag aagcgctacg
     6601 tcacgtacag ccccggcgac atctggatgg tcggcgatct cgtcaaggac ggcgtcaagg
     6661 tcgaggcccg ccccgaggaa gagcagagct tcctcgcgca gatcttcatc tcgtggttcc
     6721 cgatgctgct gctgatcggc gtgtggatct tcttcatgcg ccagatgcag ggcggtggca
     6781 ggggaggtgc attcagcttc ggcaagagca aggcgcgcat gctcgacgag tcgaacaaca
     6841 ccgtcacgtt cgccgacgtc gccggttgcg acgaggcgaa ggaagaagtc ggcgagctcg
     6901 tcgaattcct gcgcgacccg tccaagttcc agaagctcgg cggccgcatt ccgcgcggcg
     6961 tgctgatggt cggctcgccg ggcaccggca agacgctgct cgcgaaggcg atcgccggcg
     7021 aggcgaaggt gccgttcttc tcgatctccg gctccgattt cgtcgagatg ttcgtcggcg
     7081 tcggtgcggc gcgcgtgcgc gacatgttcg agcaggcgaa gaag
//
LOCUS       DASSVX010000014         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_117128, whole genome shotgun sequence.
ACCESSION   DASSVX010000014 DASSVX010000000
VERSION     DASSVX010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117128"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VFO53_00680"
     CDS             complement(<1..810)
                     /locus_tag="VFO53_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HET8874814.1"
                     /translation="MRAHKLRILSQPFRAAARAFAHLISPGQALAIAVLGLAGVAAFG
                     ITPDTAVDTPFVRTISRTLALPAVTDAEPADQRYWREERVERGDTIGSLLARASVVDP
                     DAMAFLRADPAARALYQLKPGRPLQVATDDEGRLTGLRFLAGNGELLSIARSASGFVA
                     KREAPPDDTRLTLRAGEIESSLFAAADAADLPDSVTLALTDIFGGEIDFYHDLRRGDR
                     FAVLYETRYVDGEPAGTGRVVAAEFDNAGRTHRAYLWRSPDGTEGYYNEDGR"
     gene            938..2140
                     /gene="tyrS"
                     /locus_tag="VFO53_00685"
     CDS             938..2140
                     /gene="tyrS"
                     /locus_tag="VFO53_00685"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET8874815.1"
                     /translation="MAFDIDHQIAVFRRGAAELLVESELRARLARGKPLNIKEGFDPT
                     RPDLHLGHTVQFNKLRQLQDLGHHVVFLVGDFTTLIGDPTGRNVTRPPLSAEEVAVNA
                     KTYTDQAFLILDRDKTEVAFNSKWLGALGSDGMIRLAAKYTVARMLEREDFAKRYAAE
                     QPIAIHEFLYPLAQGYDSVALKSDVELGGTDQKFNLHVGRELQRHYGQEPQTILTLPL
                     LEGLDGVSKMSKSLDNYIGITEPPASMYGKLMSISDPLMWRYYELLSFREEAQIATLR
                     RECDAGRNPREAKAMLAHEIVARFHSKAAADAAEAAFDARFRQGALPQNMPEVTLHAN
                     GAAMPIAQLARQAGIVESTSEALRLIAQRGLKVDGDVVADKSFAIAPGATVIVQAGKR
                     RFARVTIK"
BASE COUNT          430 a          793 c          790 g          380 t
ORIGIN      
        1 gcggccatcc tcgttgtaat agccttccgt gccgtcgggc gagcgccaca ggtaggcccg
       61 atgcgtcctg ccggcgttgt cgaactcggc cgcgacgaca cgtcccgtgc ccgccggctc
      121 gccgtcgacg taccgcgttt cataaagcac cgcgaagcga tcgccacgac gcaggtcgtg
      181 atagaagtcg atctcgcccc cgaagatgtc ggtgagcgcg agcgtgacgg aatcgggcag
      241 gtcggcggca tcggccgccg cgaacagcga cgattcgatt tcgccggcgc gcagcgtgag
      301 gcgcgtgtcg tcgggtggcg cttcgcgctt cgccacgaag ccgctggcgc tccgcgcaat
      361 cgacaaaagc tcgccgttgc ccgcgaggaa gcgcaagccc gtgagccggc cctcgtcgtc
      421 ggtggccact tgcagcgggc ggcccggctt cagctgatac agcgcgcgtg ccgcagggtc
      481 ggcgcgcagg aacgccattg catcgggatc gaccaccgag gcgcgcgcca gcaggctgcc
      541 gatcgtgtcg ccccgctcga cgcgctcctc gcgccagtac cgctgatcgg ccggctcggc
      601 gtcggtgacg gcggggagcg cgagcgtacg cgaaatcgtg cgcacgaagg gcgtgtcgac
      661 cgcggtgtcg ggcgttatgc caaatgcggc gacgccggcg agaccgagga cggcaatggc
      721 aagtgcctgt ccgggcgaga tgaggtgcgc gaacgcgcga gcggcggcgc gaaacggctg
      781 cgatagaatg cggagtttgt gcgcgcgcaa cggtcgacag ggcatgtttg cgggaaaatc
      841 gcgcaccgtt tcacttccgc cggcgcccga accggggccg attctaacaa gcttcgcgta
      901 aaaagcctac cgccgaatcg gcgcgatcct acagacaatg gcctttgaca tcgaccacca
      961 gatcgcggta ttccgccgcg gcgccgccga gttgctggtc gaaagcgaat tacgggccag
     1021 gcttgcgcgc ggcaagccgc tcaacatcaa ggaaggcttc gatccgacgc ggcccgacct
     1081 gcatctcggc cacacggtgc agttcaacaa gctgcgccag ctgcaggacc tcgggcacca
     1141 cgtcgtcttc ctcgtcggcg acttcacgac gctgatcggt gacccgaccg ggcgcaacgt
     1201 gacgcggccg ccgctgtccg ccgaggaagt cgcggtcaat gctaagacgt acaccgacca
     1261 ggcgttcctg atcctcgacc gcgacaagac cgaggtcgcc ttcaattcga aatggcttgg
     1321 cgcgctcggc tcggacggca tgatccggct tgccgcgaaa tacacggtcg cgcggatgct
     1381 cgagcgcgag gatttcgcga agcgctacgc cgccgagcaa ccgatcgcga tccacgaatt
     1441 cctgtacccg cttgcgcagg gctacgactc ggtggcattg aaaagcgacg tcgagctcgg
     1501 cggcaccgac cagaaattca acctgcacgt cggacgcgaa ctgcagcgtc attacggcca
     1561 ggagccgcag acgatcctga cgctgccgct gctcgagggc ctcgacggcg tcagcaagat
     1621 gtcgaaatcg ctcgacaact acatcggcat caccgaaccg ccggcgtcga tgtacggcaa
     1681 gctgatgtcg atctccgatc cgctgatgtg gcgctactac gagctgctgt cgtttcgcga
     1741 ggaagcgcag attgcaacgc tgcggcgcga atgcgatgcg ggccgcaacc cgcgcgaggc
     1801 gaaggcgatg ctcgcgcacg aaatcgtcgc gcgctttcat tcgaaggcgg cggccgacgc
     1861 ggcggaggcc gctttcgatg cccgcttccg ccagggcgcg ctgccgcaga acatgcccga
     1921 agtgacgctg catgcgaacg gcgccgcgat gccgatcgcc cagctcgcgc gccaggcggg
     1981 catcgtcgaa tcgacgtccg aggcgctgcg gctcattgcg cagcgcggat tgaaggtcga
     2041 cggcgatgtc gtcgccgaca aaagcttcgc gatcgcgccc ggtgcaacgg tcatcgtgca
     2101 ggcgggaaag cgcaggttcg cccgggtgac gatcaaataa atggggttat tcacgaatgg
     2161 ggtcagagac gacttattca agcgacgcgc gaataagtcg tctctgaccc catttattta
     2221 cattcatgcg agcccgggat cgctgccgat gccctcgagc ttcggcgcgt tcagcgcgcg
     2281 cggcttgatc gttttctgcg cgcgcagata gcgcgctacc acctcccata ccggctcgcc
     2341 gccggcggcc ttcgcctctt ccgctaccgg tgcccagccg gccaccgtgt atc
//
LOCUS       DASSVX010000015        13371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1616830, whole genome shotgun sequence.
ACCESSION   DASSVX010000015 DASSVX010000000
VERSION     DASSVX010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 13371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13371
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616830"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /gene="carA"
                     /locus_tag="VFO53_00690"
     CDS             complement(<1..1137)
                     /gene="carA"
                     /locus_tag="VFO53_00690"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET8874816.1"
                     /translation="MTDPAITPGARALFAAQPQALLALADGTVLRGRAIGAMGHATGE
                     VVFNTSMTGYQEILTDPSYAGQIVTLTYPHVGNVGINPDDAESRRIFAAGLVIRDLPK
                     LASSWRRAIDLSQYLRDAGVVGIADVDTRKLTRILRERGAQNGCVVAAESLSESDAED
                     AVARARAAPAMTGLDLAQVVTCASGYAWTSGAWALGSGYRAMGAPKFHVVAYDFGAKH
                     NILRLLAQRGCRLSVVPAKTTANEVLALAPDGIFLSNGPGDPEPCDYAVGAIRELVER
                     GLPTFGICLGHQLLGLASGARTVKMKFGHHGANHPVLDKDTGQVLITSQNHGFAVDAA
                     TLPSNVRVTHVSLFDGSLQGIARTDRPAFSFQGHPEASPGPHDLG"
     gene            complement(1166..2014)
                     /gene="dapB"
                     /locus_tag="VFO53_00695"
     CDS             complement(1166..2014)
                     /gene="dapB"
                     /locus_tag="VFO53_00695"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET8874817.1"
                     /translation="MSSASETGATGMTVRVGIAGAGGRMGQALIEATLDADDLTLAAA
                     IDLPRSADIGADAGERCGRSTGVRIVSDVGAAVGVCDVLIDFTRPAGTLAHLAACADA
                     GVGAIVGTTGFDEAGKAQIAGRARTIPIVFAPNMSVGINVLAELVRRAAQLLGPAFDI
                     EIVEMHHRLKVDAPSGTALALGEAAAAGAGVALRDHALYAREGVTGERKPGTIGFATL
                     RGGDVVGEHTVLFAGAGERLELTHRATSRRNFAVGALRAARFVAALRNRGEPGLFGMA
                     DVLGIR"
     gene            complement(1998..2495)
                     /locus_tag="VFO53_00700"
     CDS             complement(1998..2495)
                     /locus_tag="VFO53_00700"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HET8874818.1"
                     /translation="MIPTLFRWFPRAVARAAVRILPLALIPFLAAGCATFDRYAPSWR
                     SFGVYKIDINQGNYLSQDAVDRLKVGMTQAQVRQTLGTPLLTSPFRSDQWYYVYEYAR
                     RGQVVEHRNFTVYFDDGKLARWEGDKLPESVIELNRSASAKALPSTQTGDKGFWDRFL
                     DVFRK"
     gene            2548..2988
                     /gene="fur"
                     /locus_tag="VFO53_00705"
     CDS             2548..2988
                     /gene="fur"
                     /locus_tag="VFO53_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HET8874819.1"
                     /translation="MPNSHDLKSAGLKATVPRLRIINLFETSKVRHLTAEDVYRLLLA
                     EGLDIGLATVYRVLTQFEQAGLLVRHHFESGKAVFELNEGKHHDHLVCMQCGRVEEFY
                     DPEIEKRQMRVARERGFAISEHALYLYADCTKPDCPHRKNSGPN"
     gene            complement(3001..4590)
                     /gene="murJ"
                     /locus_tag="VFO53_00710"
     CDS             complement(3001..4590)
                     /gene="murJ"
                     /locus_tag="VFO53_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET8874820.1"
                     /translation="MNLLRTLTTVSGMTFLSRITGLLRETLKATVFGAGLQMDAFEAA
                     FRLPNLLRRLFGEGAFSQAFVPILAEYRRKRGLDATRALVGNVSTLLAVVLIALSVLG
                     SLAAPWLVYLLAGGFARTPGKVELTAEMIRIVFPYILFVSLVSLAGGVLNVFRRFAIP
                     AFTPVLLNVSIIGAAIVLARFVDPPIVALAIGVAIGGVAQLALQIRPLLAIGMLPKPS
                     FAWRDEGVRRVLLAMGPAVIGVSAAQISALINTQLAALLGDGRISWITYADRLMEFPS
                     ALLGVALGTILLPSLAQHHSDDDHEAYSALLDWGLRLAILMALPAAIALALLALPLVS
                     TLYQYGRFTINDVMQTRAALLGYSLGLPAIILVKILAPGFYARQVMKTPVKIASFTVV
                     VTQTLAVIFAWPLGLEQAGLTLAMSIGACCNAGLLLWLLLRKRFYEPRPGWAAFFAKV
                     CVASAVLGIVIVWLMGADSGWLHAGAIRKVGHLALVIGCAALAYFATLFALGFRLADF
                     NRREAVQSPDHVPAESGDGQE"
     gene            4690..4953
                     /gene="rpsT"
                     /locus_tag="VFO53_00715"
     CDS             4690..4953
                     /gene="rpsT"
                     /locus_tag="VFO53_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET8874821.1"
                     /translation="MANTAQARKRARQAEVTRQRNASQKSALRTAVKKVKKAIAAGDK
                     AAAVATFQSSQAVIDRIADKRIVHKNLASRTKSRLAQAIKALA"
     gene            5087..6847
                     /locus_tag="VFO53_00720"
     CDS             5087..6847
                     /locus_tag="VFO53_00720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET8874822.1"
                     /translation="MEHAATRDAIALWERHFRDPEASAAQAEERLAGTPIETADACAW
                     YALTLAFHHLFFTARPPDARRFLADAKRGFQQNGDRRGELLAAIGAARLAIVEREPDA
                     ARQQLMALYAEAQQALPPEDRFWLLNAIGAAHYFTDRLDDAIRYLYEAFEALRGVARS
                     PQHPAAMSNLAASLVTVGDYAPARELAEAALAELAHFNNPQLELFARSNLAEALAGLG
                     EYERALGVVDAMLAVDAKSTRRATQNHYLSIAAEVYAWHGRLDDAARCAQSARAILQQ
                     FPGGFNEVHSLWADAVVADARAGADVIARLTGAAEAAASHRHLPGEAKAWSRLAERHA
                     RMRNFEAAYECAKRLLAAQTQRLSHRASAKYYLLRVEHELTHARAERDRALAQRRETE
                     RLNEELARLNADLSHKMIQVEALQAQLAMEAVHDPLTQLFNRRYLDSVTPGLIGSAHR
                     RGAPLAVALLDLDRFKLVNDRHGHPAGDVVLREIGRVLPMSTRPADVVCRYGGEEFCV
                     VLPDADGPGAARALASVAARLRDLRVAWNGETLGGFTFSAGIAVLGRDGTTLAELLAA
                     ADRALYAAKDAGRDRVLMAS"
     gene            6948..7871
                     /gene="argF"
                     /locus_tag="VFO53_00725"
     CDS             6948..7871
                     /gene="argF"
                     /locus_tag="VFO53_00725"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET8874823.1"
                     /translation="MVDSKPRHFLQFSDFSRAEFDHLFERTRWIKERFKRYELYQPLK
                     DRTLAMVFEKASTRTRVSFEAGMNQLGGIAINLNRGDTQLSRGEPIEDVARVVSRMVD
                     VVMIRTFEQSIIERFAAHSRVPVINGLTNEYHPCQILADIYTYVEHRGSIAGRTVAWI
                     GDANNVCATWLQAATILDFRVHVSTPPGYALPPDAHDERHCVVFDDPEAACEGADLVT
                     TDVWTSMGFEAENDARKEAFAQWQVDATMMRRARPDALFMHCLPAHRGEEVSADVLDG
                     PQSVVWDEAENRLHAQKALLEYLVCGTVRDK"
     gene            7888..8847
                     /locus_tag="VFO53_00730"
     CDS             7888..8847
                     /locus_tag="VFO53_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET8874824.1"
                     /translation="MLKPLAVAAFALALAPLVSAQAWPEHPIKFVMSAPAGSSIDVLG
                     RTIADKLKDRLGQPVVVENKPAAGGTLATAEVARSAPDGYTMVLAFNGPLSFAPLLQK
                     LPYDVQKDLAPVIITSSQPNVLAINASLPVHSVGELVAYAKANPGKLNYASVGNGSSS
                     HLNMELFKSVAGFDAVHIPFNGSPPAVKATVQNETQMMFAVMQPLQAQIQAGALRALA
                     VTTAKRFPLLPDLPTIAESGYPGFEALAWNGVLVAAGTPKPIIARLNAEMNAILKEPD
                     VAQKMHGFGFDLIGGTPEDFGTLISGEATKWAPVIRKLGLKVD"
     gene            8865..10139
                     /locus_tag="VFO53_00735"
     CDS             8865..10139
                     /locus_tag="VFO53_00735"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET8874825.1"
                     /translation="MHDSNTAAAGAALPSRPSAPPGKVVLAYSGGLDTSVILKWLQDT
                     YRCEVVTFTADIGQGEEVEPARQKALQLGIAKQNVHIEDLREEFVRDFVFPMFRANAV
                     YEGEYLLGTSIARPLIAKRLVEIAKACGADAIAHGATGKGNDQVRFELGAYALAPNIR
                     VIAPWREWDLLSREKLLAYADRHGIPVDFKKRAGAAPYSMDANLLHISYEGGVLEDPQ
                     AEPEESMWRMTVSAEKAPDTPQTVELAYRHGDIVAIDGTEMSPAAILTALNDIGGRHG
                     VGRLDMVENRYVGMKSRGCYETPGGTIMLTAHRAMESITLDREVVALKDDLAPRYARL
                     IYNGYWFSPERKLLQGLIDASQAPVNGTVRIRLYKGSVMCVGRASPTDSLFDARISTF
                     DDDAGAYDQKDAAGFIKLNALRLRIAANRQRT"
     gene            complement(10144..10566)
                     /locus_tag="VFO53_00740"
     CDS             complement(10144..10566)
                     /locus_tag="VFO53_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874826.1"
                     /translation="MATIRHTQAALALMLAATVGSSALAQTDSTSARYPGTDYLNGGI
                     GTEQADRMRAMSADFPVQMTFAERNQGSDEFVADVHLRVTDGSGRTVIDLPSQGPIFL
                     LQVPPGSYTVEAEHEGTVKTQRFDVAAGRHDRLGFEWQ"
     gene            10680..11552
                     /locus_tag="VFO53_00745"
     CDS             10680..11552
                     /locus_tag="VFO53_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET8874827.1"
                     /translation="MPELPDLAVYVDALDTRVRGQVLRAVRVCNPFVLRTVDPPLDAV
                     AGRMVTGVRRQAKRIVIELAGELFLVVHLMIAGRLRWLEAGRAAPARITLALFEFASG
                     TLALTEAGTKRRASLHVIRGAAALAALNPGGLEVADADAKTFAARLRAENHTLKRALT
                     DPRIVSGIGNAYSDEILHRAKLSPIAHTAKLDDAAMARLFAAATGVLAEWTQRLRLEA
                     GGRFPEKVTAFRPAMAVHGRFRQPCPVCGTPVQRIVYAENECNYCPRCQTNGVVLADR
                     ALSRLLHKSFPREI"
     gene            complement(11564..12514)
                     /locus_tag="VFO53_00750"
     CDS             complement(11564..12514)
                     /locus_tag="VFO53_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8874828.1"
                     /translation="MIPLADLQSIYSVAEVDRALEDGAAKRNEGLKSWYGRMRELGGS
                     RYIIKPSTAGAVDNLYAAAPNFGQVVDDLRKFLALAVAGNEAVQFTPMLLLGEPGLGK
                     TYFAKRLAQALATGFEFVSMSSLTAGWVLTGASSQWHNARPGKVAQTLIEGEYANPVV
                     VLDEVDKAGGDTRYDPMGALYGLLERDTAVHFKDEFIDVDMDASHILWVATANDESAI
                     PEPILNRMNVYEIERPDADGSRMIALAVYREILDAHRWPFPPEPSEAVLEKLAAIPPR
                     DMRKLLLDAFGTAQLAGRDQLLPEDVDGRKLCGRRARMGF"
     gene            12867..>13371
                     /locus_tag="VFO53_00755"
     CDS             12867..>13371
                     /locus_tag="VFO53_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HET8874829.1"
                     /translation="MPAQPIAMRRIREVLRLKHECALSNAQIAAAVRIAKGSVANYLA
                     AAEAAGLTYKESLALDDAALAARLQPQRHGQSQFAQPDFGHVHRELRRKGVTLQLLWE
                     EYRAQATGIPYSRSRFCERYLEFRQHLRRSMRQTHVAGEKLFVDFAGPTVPIIDAASG
                     EISRAHIF"
BASE COUNT         2261 a         4541 c         4506 g         2063 t
ORIGIN      
        1 gccgaggtcg tgcggaccgg gcgaggcctc cgggtggccc tggaagctga acgcggggcg
       61 atcggtgcgg gcgatgccct gcaggctgcc gtcgaacagc gacacgtgcg tgacgcgcac
      121 attcgacggc agcgtcgcgg cgtccaccgc gaagccgtgg ttctggctcg tgatcagcac
      181 ctgcccggtg tccttgtcga gcaccgggtg gttcgcgccg tgatggccga atttcatctt
      241 gacggtgcgc gcgcccgacg cgagtccgag caactggtgc ccgaggcaga tgccgaacgt
      301 cggcaggccg cgctcgacga gctcgcgtat cgcgccgacc gcgtagtcgc agggctcggg
      361 gtcgccgggg ccgttcgaca ggaagatgcc gtcgggcgca agtgccagca cctcgttcgc
      421 ggtggttttc gcgggaacca ccgaaaggcg gcagccgcgc tgcgcaagga gccgcaggat
      481 gttgtgcttg gcgccgaaat cgtacgcgac gacgtggaat ttcggcgcgc ccattgcgcg
      541 gtagccgctg ccgagcgccc acgcgcccga cgtccacgcg taaccggatg cgcacgtcac
      601 gacctgcgcg agatcgagcc cggtcatcgc tggcgcggcg cgggcgcgcg ccacggcgtc
      661 ctcggcatcg gactcgctca acgactcggc cgcgacgacg cagccgttct gtgccccgcg
      721 ctcgcgcagg atgcgcgtca gcttgcgtgt atcgacatcg gcgatgccga cgacgccggc
      781 gtcgcgcaga tactgcgaca agtcgatcgc gcgccgccag ctcgatgcga gcttcggcag
      841 gtcgcgaatc acgaggcccg ccgcgaaaat gcgccgcgac tcggcgtcgt cgggattgat
      901 gccgacattg ccgacatgcg gatacgtgag cgtgacgatc tgccccgcgt acgacggatc
      961 ggtcaggatc tcctggtaac cggtcatcga cgtgttgaag acgacctcac cggtcgcatg
     1021 acccatcgca ccgatcgcac gtccgcgcaa tacggtgccg tcggcgagcg ccagcagcgc
     1081 ctgcggctgt gcagcgaaca gcgcgcgcgc gccgggcgtg attgccgggt cggtcatgaa
     1141 ggcgcgatcg cggtgggcgc gcgcgctagc ggatgccgag cacgtccgcc atgccgaaca
     1201 ggccgggctc gccgcggttg cgcagcgcgg cgacgaagcg cgcggcgcgc agcgcgccca
     1261 cggcgaaatt gcgcctcgac gttgcgcggt gcgtgagctc gagccgctcg ccggccccgg
     1321 cgaagagcac cgtatgctcg ccgacgacgt cgccaccgcg cagcgtcgcg aagccgatcg
     1381 ttcccggctt gcgctcgccg gtgacgcctt cgcgggcgta gagcgcgtgg tcgcgcagcg
     1441 cgacaccggc gccggccgcc gctgcctcgc caagtgccag cgcggtgccc gacggcgcat
     1501 cgaccttgag ccgatgatgc atctcgacga tctcgatgtc gaacgccggt ccgagcaatt
     1561 gcgccgcccg cctcacgagt tcggccagca cgttgatgcc gacgctcatg ttcggcgcga
     1621 agacgatcgg gatggtgcgg gcgcgcccgg cgatttgcgc cttgcccgcc tcgtcgaagc
     1681 cggtggtgcc gacgatggca ccgacccccg catcggcgca cgcggcaaga tgcgcgagcg
     1741 ttcccgcggg gcgcgtgaaa tcgatcagca cgtcgcaaac gccaacggcg gcaccgacgt
     1801 ccgacacgat gcgcacgccg gtcgagcggc cacaccgctc gcccgcgtcg gcgccgatgt
     1861 cggcgcttcg tggcaggtcg atcgccgcgg ccagcgtgag atcgtcggcg tcgagcgtgg
     1921 cctcgatcag tgcctgcccc atccggccgc cggcgccggc tatgccgacg cgaaccgtca
     1981 tgccggtggc gcccgtctca cttgcggaag acatcgagga agcgatccca gaagcccttg
     2041 tcgcccgtct gcgtggacgg cagcgccttg gcgctcgcgg agcgattgag ctcgatcacc
     2101 gactcgggca gcttgtcgcc ttcccagcgc gcaagcttgc cgtcgtcgaa atagacggtg
     2161 aaattgcgat gctcgacgac ctgtccgcgg cgcgcatact cgtacacgta ataccactgg
     2221 tcggagcgga acggcgacgt gagcagcggc gtgccgaggg tctggcgcac ctgcgcctgc
     2281 gtcatgccca ccttgagcct gtccaccgcg tcctgcgaca ggtaattgcc ctggttgatg
     2341 tcgatcttgt agacgccgaa cgagcgccag gacggcgcgt agcgatcgaa cgtcgcgcag
     2401 cctgccgcaa ggaacgggat gagcgccagc ggcaggatgc gcacggcggc ccgcgcgacg
     2461 gcgcggggga accatcgaaa cagtgtggga atcatcaagg caaatggcta ttatagccac
     2521 gacacgcaag cgcccgctta catcgacatg cccaattcgc acgacctcaa gagtgccggc
     2581 ctcaaggcga ccgtgccgag gcttcggatc atcaacctgt tcgagacgtc gaaggtcagg
     2641 catctgaccg ccgaagacgt ctacaggctg ctgctggccg agggactcga catcgggctc
     2701 gccaccgtgt atcgcgtgct gacgcagttc gagcaggcgg gcctgctcgt ccggcatcat
     2761 ttcgagtcgg gcaaggcggt gttcgagctg aacgagggca agcaccacga tcatctggtc
     2821 tgcatgcaat gcgggcgggt cgaggagttc tacgaccccg aaatcgagaa gcggcagatg
     2881 cgcgttgcga gggagcgggg attcgcgatc agcgagcacg cgctgtatct ctacgccgac
     2941 tgtacgaagc ccgattgccc gcatcgcaag aacagcggcc ccaactgagc gcgcgtcgcg
     3001 tcactcctgt ccgtcgccgc tttcggcggg cacgtgatcc ggcgattgca cggcttcgcg
     3061 ccggttgaaa tcggcgagcc tgaagccgag cgcgaacagc gtcgcgaagt aggcaagtgc
     3121 cgcgcagccg atcacgagtg cgaggtgacc gaccttgcgg atcgcgccgg catgcagcca
     3181 tccgctgtcg gcacccatca gccagacgat cacgatcccg agcaccgcgc tcgccacgca
     3241 taccttggcg aagaacgccg cccatccggg ccgcggctcg tagaagcgct tgcgcagcag
     3301 cagccacagc agcagtcccg cgttgcagca ggcgccgatg ctcatcgcca gcgtgagacc
     3361 ggcctgctcg agaccgagcg gccacgcgaa gatgacggcg agcgtctgcg tgacgaccac
     3421 ggtgaacgac gcgatcttta ccggcgtctt catcacctgc cgcgcataaa agccgggcgc
     3481 gaggatcttc acgaggatga tcgccggcag tcccaggctg tagccgagca gcgccgcacg
     3541 cgtctgcatc acgtcgttga tcgtgaaccg gccgtactgg tacagcgtcg acacgagcgg
     3601 aagtgccagc aacgcgagcg cgatggctgc cggaagcgcc atcaggatgg caagacgcag
     3661 gccccagtcc agcaacgcgg aataggcttc gtgatcgtcg tcgctgtggt gctgcgcaag
     3721 cgacggcagc aggatcgtgc cgagcgccac gccgagcagc gcgctcggga actccatcag
     3781 gcggtcggca taggtgatcc atgagatgcg gccgtcgccg agcagcgccg cgagctgcgt
     3841 gttgatcagc gccgaaatct gcgcggccga tacgccgatc accgccggac ccatcgcgag
     3901 cagcacgcga cgcacgcctt cgtcgcgcca cgcgaacgac ggcttcggca gcatgccgat
     3961 cgccagcagc ggccgtatct gcagcgcgag ctgcgcgacc ccgccgatcg ccacgccgat
     4021 cgcgagcgcg acgatgggcg gatcgacgaa gcgcgcaagc acgatcgccg cgccgatgat
     4081 cgacacattg agcagcaccg gcgtgaacgc cgggatggcg aagcggcgga acacgttgag
     4141 caccccgccc gcgagcgaga cgagcgagac gaacaggatg tacggaaaga cgatccgtat
     4201 catctccgcg gtgagctcga cctttccggg cgtgcgcgca aacccgcctg ccagcagata
     4261 gacgagccag ggtgcggcaa gcgaaccgag caccgacaag gcgatcagca cgacggcgag
     4321 aagcgtcgac acgttgccga cgagcgcgcg cgtcgcgtcg aggccgcgct tgcgccggta
     4381 ttcggccagg atcgggacga acgcctgcga gaaggcgccc tcgccgaaca ggcggcgcag
     4441 caggttcggc agccggaatg cggcttcgaa cgcgtccatc tgcagaccgg ccccgaacac
     4501 ggtcgccttg agcgtttcgc gcaacaggcc cgtgatgcgc gacaggaacg tcatgccgct
     4561 cactgtcgtc agcgtgcgaa ggagattcaa tcgggaagct cgcggaacgc cttgcttgta
     4621 aagcgaaaat cgcgtattat gcccggtctt tcgacccgcg ttatttatca attcaggaat
     4681 cgagcagaga tggccaacac ggcacaggca cgcaagcgcg cgcgacaggc agaggtgact
     4741 cgccaacgca acgcaagcca gaaatcggcg cttcgcaccg cggtcaagaa ggtgaagaag
     4801 gccatcgcgg ccggcgacaa ggcggccgcc gtcgcgacgt tccaatcgtc gcaagcggtg
     4861 atcgaccgca tcgccgacaa gcgcatcgtc cacaagaacc tcgcgtcgcg cacgaagtcg
     4921 cggcttgcgc aggcgatcaa ggcgctcgcc taacggcgtt cgaacgcaat cacgatcagg
     4981 gcgtcccgcg gggcgccctg atcgtttcag ggaatcatgg ttgtggactg caatcgcgcg
     5041 gcaccgatga tcgcgcccgg cgaacgcctg gcatcccgct gacatcgtgg aacacgccgc
     5101 gacgcgcgac gccattgcat tgtgggagcg gcatttccgc gacccggaag cgtccgcggc
     5161 gcaggccgag gagcgcctcg ccggcacgcc gatcgagacg gcggacgcat gtgcgtggta
     5221 cgcgctcacg ctcgcgtttc atcatctgtt cttcacggcg cgcccgcccg acgcgcgccg
     5281 ctttctcgcc gacgcgaaac gcggcttcca gcaaaacggc gatcgccgcg gcgagctgct
     5341 cgccgcgatc ggcgccgcgc ggctcgccat cgtcgagcgc gagcccgacg ctgcccgcca
     5401 gcaactgatg gcgctgtacg cggaagcgca gcaggcgctg ccgccggaag atcgcttctg
     5461 gctgctgaac gcgatcggtg cggcccacta cttcaccgat cggctcgacg acgcgatccg
     5521 ctatctttat gaagcgttcg aagcgctgcg cggcgtcgcg cgctcgccgc agcacccggc
     5581 ggcgatgtcg aacctcgccg cgtcgctcgt cacggtcggc gattacgcgc ccgcacgcga
     5641 gctcgcggaa gccgcgctcg cggagctcgc gcacttcaac aatccgcagc tcgaattgtt
     5701 cgcgcgctcg aacctcgcgg aagcgcttgc cgggcttggc gaatacgagc gtgcgctcgg
     5761 cgtcgtcgac gcgatgctcg ccgtcgacgc caaaagcacg cggcgcgcca cgcagaatca
     5821 ttacctgtcg atcgccgcgg aagtctacgc atggcacggg cggctcgacg acgccgcccg
     5881 gtgcgcgcaa agcgcacgcg cgatccttca gcagttcccg ggcgggttca acgaagtcca
     5941 ttcgctgtgg gccgatgcag tcgttgccga cgcgcgcgcc ggcgccgacg tgatcgcgcg
     6001 gctcaccggc gccgccgaag cggcggcctc gcatcgccat ctgccgggtg aagccaaggc
     6061 gtggtcgagg cttgccgagc gccatgcccg gatgcgcaac ttcgaagcgg cttacgaatg
     6121 cgcgaagcga ctgcttgcgg cgcagacgca gcgcctgtcg catcgcgcga gcgccaaata
     6181 ctacctgctg cgtgtcgagc acgagttgac acatgcccgg gccgagcgcg accgcgcgct
     6241 cgcgcagcgc cgcgagacgg agcgattgaa cgaggagctc gcccggctca acgccgatct
     6301 ttcgcacaag atgatccagg tggaggcgct gcaggcgcag ctcgccatgg aagcggtgca
     6361 cgacccgctg acgcagctct tcaaccggcg ctacctcgac tcggtgacgc cgggactgat
     6421 cggctcggcc catcgtcgcg gcgcgccgct cgcggtggcg ctcctcgatc tcgaccgctt
     6481 caagctcgtg aacgatcggc acggccatcc tgcgggggac gtcgtgctgc gcgaaatcgg
     6541 gcgcgtgctg ccgatgtcga cgcgtcccgc cgacgtcgtg tgccgctacg gtggcgagga
     6601 attctgcgtg gtgctgcccg acgccgacgg gcccggcgct gcgcgcgcgc tcgcgagcgt
     6661 cgccgcccgc ctgcgcgacc ttcgcgtcgc ctggaatggc gagacgctcg gcggctttac
     6721 gttctccgcg gggatcgcgg tcctcggtcg cgacggcacg acactcgccg agcttcttgc
     6781 cgccgccgat cgggcgctgt acgcggcaaa ggatgcgggc cgcgaccgcg tgctgatggc
     6841 gtcgtgaccg tgcatcgcgc gaaaggcgca tgcgtgccgc ttcggcgtcg cgtgctaaca
     6901 tttccgattc gttgaaggtc gcagcccatg ccggccgcga cgcgtcgatg gtcgattcga
     6961 agccccggca tttcctgcag ttcagcgatt tttcgcgggc cgaattcgac catctgttcg
     7021 agcgcacccg ctggatcaag gagcgcttca agcgctacga gctctaccag ccgctcaagg
     7081 accgcacgct tgcgatggtg ttcgagaagg cgtcgacgcg cacgcgcgtt tcgttcgagg
     7141 ccggcatgaa ccagctcggc ggcatcgcga tcaacctcaa ccgcggcgac acccagctct
     7201 cgcgcggcga gccgatcgag gacgttgcgc gcgtcgtgtc gcggatggtc gacgtggtga
     7261 tgatccgcac gttcgagcaa tcgatcatcg agcgcttcgc cgcccactcg cgtgttccgg
     7321 tgatcaacgg gctcaccaac gagtatcacc cgtgccagat cctcgccgac atctacacgt
     7381 acgtcgagca tcgcggctcg atcgcgggcc gcacggtcgc ctggatcggc gatgcgaaca
     7441 acgtgtgcgc gacgtggctg caggcggcga cgatcctcga tttccgcgtg cacgtgtcga
     7501 cgccgccggg ctacgcgctt ccgcccgatg cgcacgatga gcgtcattgc gtcgtcttcg
     7561 acgaccccga agccgcctgc gaaggcgccg atctcgtgac gaccgacgtg tggacgagca
     7621 tggggttcga ggcggagaac gacgcgcgca aagaggcgtt cgcgcagtgg caggtggacg
     7681 cgacgatgat gcggcgcgcc cggcccgacg cgctcttcat gcactgcctg ccggcgcacc
     7741 gcggggagga ggtttcggcc gacgtcctcg acggcccgca gagcgtcgtc tgggacgagg
     7801 ccgagaaccg gctgcatgcg caaaaggcgc tgctcgagta cctcgtctgc ggcaccgtgc
     7861 gcgataaata gtgacaggga gcagacgatg ttgaagccac tggcggtcgc agctttcgca
     7921 ctggcgctcg cgccgctcgt gtcggcccag gcgtggcccg agcatccgat caagttcgtg
     7981 atgtcggcgc cggcgggaag ctcgatcgac gtgctcggac gcacgatcgc cgacaagctc
     8041 aaggatcgcc tcggccagcc ggtcgtcgtc gagaacaagc cggcggccgg cggcacgctc
     8101 gccaccgccg aggtcgcccg ttcggcaccc gacggctaca cgatggtgct cgcgttcaac
     8161 ggtccgctgt cgttcgcgcc gctgttgcag aagctgccgt acgacgtgca gaaggatctc
     8221 gcgccggtca tcatcacgtc gagccagccg aacgtgctcg ccatcaacgc gtcgctcccc
     8281 gtgcattcgg tgggcgagct cgtcgcgtat gcgaaggcga acccgggcaa gctcaattac
     8341 gcgtcggtcg gcaacggcag ctcgtcgcat ctcaacatgg agctcttcaa gagcgtcgcc
     8401 ggcttcgacg ccgtgcacat tccgttcaac ggctccccgc ccgcggtcaa ggccaccgtg
     8461 cagaacgaga cgcagatgat gttcgccgtg atgcagccgc tgcaggccca gatccaggcc
     8521 ggcgcgctgc gtgcgctcgc cgtgaccacg gccaagcgct ttccgctgct gcccgacctg
     8581 ccgacgatcg ccgaatcggg ctatcccggc ttcgaggcgc ttgcatggaa cggcgtgctc
     8641 gttgccgccg gcacgccgaa gccgatcatc gcgcgcctca atgcggagat gaacgcgatc
     8701 ctcaaggagc ccgacgtcgc gcagaagatg cacggcttcg ggttcgacct catcggcggt
     8761 acgcccgagg acttcggcac gttgatcagc ggcgaggcga cgaagtgggc gccggtgatc
     8821 aggaagctcg ggctcaaggt cgactgaaac gcatggaaac gccaatgcac gacagcaaca
     8881 ccgccgcggc cggcgccgcg ctgccgtcgc gtccttcggc gccccccggc aaggtggtgc
     8941 tcgcttattc gggcggcctc gacacgtcgg tgatcctcaa gtggctgcag gacacctacc
     9001 gctgcgaggt cgtcacgttc acggccgaca tcggccaggg cgaggaggtc gagcccgcgc
     9061 gccagaaggc gctgcagctc ggcatcgcga agcagaacgt gcacatcgag gacctgcgcg
     9121 aggagttcgt gcgcgacttc gtgttcccga tgttccgcgc caatgcggtg tacgagggcg
     9181 aatacctgct cggcacgtcg atcgcacgcc cgctgatcgc gaagcggctc gttgaaatcg
     9241 cgaaggcgtg cggcgccgat gcgatcgccc acggcgcgac gggcaagggc aacgaccagg
     9301 tccgcttcga gctcggcgcc tacgcgcttg cgcccaatat ccgcgtcatc gcgccgtggc
     9361 gcgaatggga tctgctgtcg cgcgaaaagc tgctcgccta cgccgatcgc cacggcattc
     9421 cggtcgactt caagaagcgg gcgggtgctg caccctactc gatggacgcg aacctgctgc
     9481 acatcagcta cgaaggcggc gtgctcgagg atccgcaagc cgagcccgaa gagtcgatgt
     9541 ggcggatgac cgtgtctgcc gaaaaggcgc ccgatacgcc gcagaccgtc gagcttgcgt
     9601 accgccacgg cgacatcgtc gccatcgacg gcaccgagat gtcgccggcc gcgatcctca
     9661 cggcgttgaa cgacatcggc ggccgccacg gcgtcggccg gctcgacatg gtcgaaaacc
     9721 gctacgtcgg catgaagtcg cgcggctgct acgagacgcc cggcggaacg atcatgctga
     9781 cggcgcaccg ggcgatggaa tcgatcacgc tcgaccgcga ggtcgtcgcg ctcaaggacg
     9841 accttgcgcc gcgttacgcg cggctcatct acaacgggta ctggttcagt cccgagcgca
     9901 agctgctgca ggggctgatc gacgcgtcgc aggcgccggt caacggcacc gtgcgcatca
     9961 ggctctacaa gggcagcgtc atgtgcgtcg ggcgtgcatc gccgaccgac tcgctgttcg
    10021 acgcaaggat atcgacgttc gacgacgacg ccggcgccta cgaccagaag gacgcggcag
    10081 gattcatcaa gctcaacgcg ctgcggttgc gcatcgccgc gaaccggcag cgcacgtagc
    10141 ccttcattgc cactcgaagc cgagccggtc gtgccggccg gcggcgacgt cgaagcgctg
    10201 tgtcttcacc gtaccttcgt gctccgcctc gaccgtgtac gagccgggcg gaacctgcag
    10261 cagaaaaatc ggaccctgcg acggcaggtc gatcaccgtg cgcccgctgc catccgtgac
    10321 gcgcaggtgc acgtcggcga cgaattcgtc gctgccctgg ttgcgttccg cgaacgtcat
    10381 ctgcaccgga aagtcggcgc tcatcgcgcg catccggtcg gcctgctcgg tgccgatgcc
    10441 gccgttgaga taatccgtcc ccgggtagcg tgcggacgtg gagtccgttt gcgcgagtgc
    10501 ggacgagccg accgtcgcgg cgagcatgag cgcaagcgcc gcctgcgtgt gtcgaatggt
    10561 tgccatgtcg aagactccgt cgatgcgaaa caacgacaat gatgtctcac gcgcgccgaa
    10621 tcggctgccg gcgcagaccg tgtttcgttg tcggtgcatg accgaccgtc gcgactgaca
    10681 tgccggaact gcccgacctc gccgtctacg tcgacgcact cgacacgcga gtgcgcggac
    10741 aggtgctgcg tgccgtgcgc gtttgcaatc cgttcgtgct gcgcacggtc gacccgccgc
    10801 tcgacgcggt cgcgggccgc atggtgacgg gcgtgcgccg ccaggccaag cgcatcgtga
    10861 tcgagctggc cggcgagctc ttcctcgtcg tgcatctgat gatcgccgga cgcttgcgct
    10921 ggctcgaagc gggacgcgcc gcgcccgcgc gcattacgct ggcgctgttc gagtttgcga
    10981 gcggcacgct ggcgctgacg gaggcgggca cgaagcggcg cgcgtcgctg cacgtgatcc
    11041 gcggcgccgc ggcgctggcc gcgctgaatc ccggcgggct cgaggtcgcc gacgccgatg
    11101 cgaaaacgtt cgccgcacgt cttcgcgcgg agaaccacac gctgaagcgg gcgctgaccg
    11161 acccgcgcat cgtcagcggc atcggcaacg cgtactccga cgagatcctg caccgcgcga
    11221 agctttcgcc gatcgcgcat acggcgaagc tcgacgacgc ggcgatggca aggctattcg
    11281 cggccgcgac cggcgttctc gccgaatgga cgcagcgcct gcgtctcgag gcgggcggcc
    11341 gcttccccga aaaagtgacg gcgtttcgac ccgcgatggc cgttcatggc aggttcaggc
    11401 agccgtgccc cgtctgcggc acgccggtgc agcgcatcgt gtacgccgag aacgagtgca
    11461 attattgccc gcgctgccag acgaacggcg tggtgctcgc cgatcgcgcg ctgtcgcggc
    11521 tgctgcacaa aagcttcccc cgcgaaatct gattgcgggg gactcagaat cccatgcgcg
    11581 cgcgccgccc gcagagcttg cgcccgtcga catcctcggg cagcaactgg tcgcgtcctg
    11641 cgagttgcgc cgtgccgaac gcatcgagca gcagcttgcg catgtcgcgc ggcgggatcg
    11701 ccgcgagctt ctcgagcacc gcctcggagg gctcgggcgg aaacggccag cggtgcgcgt
    11761 cgaggatctc gcggtacacg gcgagcgcga tcatccgcga gccgtcggca tcggggcgct
    11821 cgatttcgta gacgttcatc cggttgagga tcggttccgg aatcgcgctt tcatcgttcg
    11881 ccgtggcgac ccacaggatg tgcgacgcgt ccatgtcgac gtcgatgaat tcatccttga
    11941 aatgcacggc ggtgtcacgc tcgagcagcc cgtacagcgc acccatcgga tcgtagcgcg
    12001 tgtcgccgcc ggccttgtcg acctcgtcga gcacgaccac cgggttcgcg tattcgcctt
    12061 cgatcagcgt ctgcgccacc ttgccgggcc gcgcgttgtg ccattgtgac gacgcgcccg
    12121 tcagcaccca gcccgcggtg agcgagctca tcgacacgaa ttcgaagccg gttgcgagcg
    12181 cctgcgcaag ccgtttcgcg aagtacgtct tgccgaggcc cggttcgccc agcaacagca
    12241 tcggcgtgaa ctgcaccgcc tcgttgcccg cgacggcgag ggccaggaac ttgcgcagat
    12301 cgtcgacgac ctggccgaaa ttgggcgccg cggcgtaaag attatccacg gccccggcgg
    12361 tcgacggctt gatgatgtag cgcgagccgc cgagctcgcg catgcggccg taccagctct
    12421 tgaggccctc gttgcgcttg gccgcgccgt cctcgagcgc gcggtcgacc tcggccaccg
    12481 agtagatcga ctgcagatcc gcgagcggaa tcatggcgtc catgaacgta tgttgcctcc
    12541 gcgtatcaca ttgccttcgc gggtcattat gcatcacgca tgccagcttc ggcggtgtcg
    12601 ggccgcgcga gcggcgcgcg cgcgtggcgc cggcgccact gtgtattccg acgcaagttg
    12661 accgcgcaat ccggcgatgt tgaccgctga ttccgaccga agctgaccga ttttccggtc
    12721 gcttcggaaa tggcggtcaa gatggtcgga tcgccggtca cgatcgtcgg aaatgacggt
    12781 cttggcggac tcggcggcgt tgcagtgcgt ggcaatgcga ctttttggct cggtaccctc
    12841 gctccctttt gtccaaggag cccgtggtgc cagcccagcc gattgccatg cgacgaattc
    12901 gcgaagttct gcgcctcaag cacgagtgcg cgctgtcgaa cgcccagatc gccgccgccg
    12961 tgcggatcgc caagggctcg gtcgccaact acctggcggc cgccgaagcc gccgggctga
    13021 cctacaagga atcgctcgcg ctcgatgacg ccgcgctcgc ggcacggctg cagccgcaac
    13081 ggcacgggca gagccagttc gcccagcccg atttcgggca tgtgcaccgc gagttgcggc
    13141 gcaagggcgt cacgctgcag cttctctggg aggaatatcg cgctcaagcg acgggcattc
    13201 cctacagccg gtcgcggttc tgcgagcgtt acctggaatt ccggcaacac ctgcgtcgtt
    13261 cgatgcggca gacgcatgtc gccggcgaga aattgttcgt tgacttcgcc ggcccgacgg
    13321 tgccgatcat cgacgccgcg agcggcgaaa tctcgcgcgc ccacatcttt g
//
LOCUS       DASSVX010000016         7585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_767015, whole genome shotgun sequence.
ACCESSION   DASSVX010000016 DASSVX010000000
VERSION     DASSVX010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767015"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VFO53_00760"
     CDS             <1..473
                     /locus_tag="VFO53_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8874830.1"
                     /translation="IRASQRTRVRDFLDDAKRSGLTTVATGRIADDAPAGGYYVAPTL
                     LRDVPFEHRLARDEVFGPVLAAMAFDDEAHAIALANATDYGLVAGIWTRDGGRQLRMA
                     RALSSGQVFVNNYGAGGGVELPFGGVKHSGYGREKGFEALHGFTTLKTVVLRHG"
     gene            466..1299
                     /locus_tag="VFO53_00765"
     CDS             466..1299
                     /locus_tag="VFO53_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET8874831.1"
                     /translation="MDDAAAVRPFSIVELGHKLVGRAVVYALFPVLLSQAFALRRHAL
                     TLVPAAGPATGRIGRGEPLRFLAIGDSIIAGVGARRVERSTVVHVARFMSGRLAREIN
                     WHASGMIGAGARRVRREVVPQLPAQAFDVILLSVGVNDVLKLERKGRFRRQLLKLIRE
                     LQRHSPHAVIGFLGLPPLDEFPKLRRPLRWLVGHRVRHFDAVAREALRRVPNAMHIPV
                     RFSPRPDMFSDDGLHPSETSQRRLAKMIAGALTPSLSHGAVGVAIRARHSHDPEPGHI
                     Q"
     gene            1296..2051
                     /locus_tag="VFO53_00770"
     CDS             1296..2051
                     /locus_tag="VFO53_00770"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET8874832.1"
                     /translation="MRLENKVAIVTGAASGFGAGIAKRFAAEGARVVVNDLHADGATR
                     MAVAIEQAGGRAVACAGDVSKDADVARLVASAIDAFGRLDVVVNNAGTTHRNQPMLDV
                     PEEMFDRIYAVNVKSLYLTARHAVPHFRAQRNGAFITIASTAGVRPRPGLTWYNGSKG
                     AAIVTSRSMAAELAPHNIRVNIINPVAGETAMLADFMGEDTPQKRAQFIATIPLGRLS
                     QPSDVATAAVFFASDEAAFITGTCMEVDGGRCI"
     gene            2142..3323
                     /locus_tag="VFO53_00775"
     CDS             2142..3323
                     /locus_tag="VFO53_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HET8874833.1"
                     /translation="MKTDSAAMPLLLGALIATAPFAMDVYLASMPSMTHALGATTAEV
                     QLTLSVYMYGWGVAQLFAGPLSDRFGRRPVLLASLVVFVIASILCALSRNVTVLIAGR
                     LVQALSMASIAVVPRAVVRDLYGGDKAAHILSLMGVVLGIAPVVAPLIGSNLHVWLGW
                     QSNFVLVALYGAILWCCVYKVLPETLARPDARATAPAVIAANYRRLLRSRSYVGFMLV
                     AAFGFSGLFAFLAGSAFVFVSVMGKGEQGFGVMFGTVMLGNITGATVGSRLVRRIGIA
                     RQLRLGTALMLAAGLAIGAPALLGVTHPLAVVVPMFAFMVTFTLTMPAATAGALTPFP
                     QIAGSASSLLAFCQFVVASTAALVVGLTLDGTTRPMALTIAVASLGAFVSLRIAHREA
                     I"
     gene            complement(3330..4538)
                     /locus_tag="VFO53_00780"
     CDS             complement(3330..4538)
                     /locus_tag="VFO53_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET8874834.1"
                     /translation="MTPSAASSRPLAGIRVLEMGALIAGPFCAKILAEFGADVVKLEP
                     PGSGDPLRNWRYVENGTSLWWHVQSRNKRSVAVDLRTPDGQAIARALALRADILVENF
                     RPGTLEGWNLAYESLAAANPGLVMVRISGYGQTGPYRERPGFGVIGEAVGGLRHVTGT
                     PDRPPSRVGVSIGDTLSALYGVIGAMMALESRRSTGRGQVVDVALYEAVFSVMESMLP
                     EFDRFGAVRERTGSILPGIAPTSAYRCADASFVLIAANGDSIFQRLCRAIGRDDLASD
                     PSLAHNDGRAARQQWLDGEIEQWTVRRAPEEVVAVMQTADVPASKIYTIRDIVADAQF
                     AAREMIRDVRLHDGSRLAVPGVVPKLSQTPGGFDGGGPRLGEHTDAVLRDLGYDADAI
                     ADLRRRRVID"
     gene            4653..5162
                     /locus_tag="VFO53_00785"
     CDS             4653..5162
                     /locus_tag="VFO53_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HET8874835.1"
                     /translation="MLASFDRRLLAVNRVLVIVLLAAMAIMVFANVALRFLTDHSILW
                     AEEASRYAMVWLTFIGAGPVLRYGGHIGIDTLEHVSPRAAPAIRGVILTLLAVFFCVM
                     IVVGIRYASLTWAQTTPVLEVPIGAVYLAMPIGSALLLVHLALMAHGYVARRELLGDG
                     EFDADAAKL"
     gene            5178..6461
                     /locus_tag="VFO53_00790"
     CDS             5178..6461
                     /locus_tag="VFO53_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET8874836.1"
                     /translation="MVTILFVACALLLALGVPVAFALGGGTLAAMLWQHVPLVALPKY
                     IFSGIDLFALEAVPFFILTAELMTGGELSAVLLRFASQFVGRFRGGLGHTNILTLTFF
                     SGISGSALADAAGPGAIMIRMMRDDGYDAGYAAALTSATSILGPIIPPSISVIIYALA
                     YPDVNLVGLLIAGVVPGILIAAAMAAINHVISVRRNYRGAAERLGWREYVRNTWRALP
                     ALVLPVLILGGIASGAFTPTEASVVAVFYALFAGRVIYRTLQWRMVPAILARSALMTA
                     SVLLIVGMSAAFAWVLTVSRVPQDLTDWIVGMQLPPLAFLLAVNVLLLVFGIFIEPLP
                     GIVVLVPILAPIAHALHIDDLQFGIVVIVNLTMGMITPPVGGLLFVTSIVARVPLARL
                     VKELWPFLWAQLVVLALLSLFPVLSSGLPNFFGYR"
     gene            complement(6469..7461)
                     /locus_tag="VFO53_00795"
     CDS             complement(6469..7461)
                     /locus_tag="VFO53_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET8874837.1"
                     /translation="MAMTAIPPSARLWQLATGHFLPRCLHVVAELGVADYLGDTPATA
                     EALAAAAGANADALERMLRLLAVAGIFEARATLWTHTELSRLLRSDHPQSMRPFVRMI
                     GGRMQWTAAGELEYAGRTGEAAIEKLVSGGLWAYFRDHPEEARIFDAAMTGKAVAEIR
                     ALLPAFDFSRYGVIADVGGGRGHVLRAVLDAAPGAQGVLFDQPSVVGALAPSARMTFR
                     GGDFFKDPLPEADAYLLSNVLHDWADREAERILRAVRRSAPRHAEVLVLESPLPEGGE
                     AHHAKVLDIVMLALTGGRERTQREYQALFEAAGFRLDRVVATAGPFSVIVGVPA"
BASE COUNT         1062 a         2606 c         2562 g         1355 t
ORIGIN      
        1 tgatccgcgc atcgcagcgt acgcgcgtgc gcgacttcct cgacgatgcg aagcgaagcg
       61 gccttacgac cgttgcaacc ggccgcatcg ccgacgacgc acccgccggc ggctactacg
      121 tcgcgccgac gctactgcgc gacgtgcctt tcgagcatcg acttgcccgc gacgaggtgt
      181 tcggcccggt actcgccgcg atggcgttcg atgacgaagc gcatgcgatt gcgctcgcga
      241 acgcgaccga ttacgggctc gtcgccggca tctggacgcg cgatggcgga cggcaactgc
      301 gcatggcgcg cgcgctttcg agcgggcagg tattcgtcaa caactacggc gcgggcggcg
      361 gcgtcgagct gccgttcggc ggcgtgaagc actcgggtta cggccgcgaa aagggcttcg
      421 aggcgctgca tggattcacg acgctgaaaa cggtcgtcct gcggcatgga tgatgcggcc
      481 gcggtgcggc cgttctcgat cgtcgagctc ggccacaagc tcgtcggccg cgcggtcgtg
      541 tacgcgctgt tcccggtgct cctgtcgcag gcgttcgcgc tgcgccgtca tgcattgacg
      601 ctcgtgcctg cagccggtcc cgcgacgggc aggatcggcc gcggcgagcc gctgcgcttc
      661 cttgcgatcg gcgattcgat catcgccggc gtcggcgcgc gtcgcgtcga gcgctcgacg
      721 gtggtccacg tcgcgcgctt catgtccggc cgccttgccc gcgagatcaa ctggcacgcg
      781 tcggggatga tcggcgccgg tgcaagacgc gtgcgccgcg aggtggtgcc gcagctgccg
      841 gcgcaggcct tcgacgtgat cctgctctcg gtcggcgtga acgacgtgct gaagctcgag
      901 cgcaagggcc gcttccggcg ccagttgctg aagctgatcc gcgaattgca gcggcattct
      961 ccgcacgcgg tgatcggctt cctcgggctg ccgccgctcg acgagtttcc gaagctgcgc
     1021 cggccgctgc gctggctcgt cggccaccgc gtccggcatt tcgacgccgt tgctcgcgag
     1081 gcgctgaggc gcgtgcccaa tgcgatgcac attccggtgc gcttctcgcc gcgccccgac
     1141 atgttctcgg acgacgggct gcatccctcc gaaaccagcc agcgccggct tgcgaagatg
     1201 attgccggtg cgctgacacc ttcgctgtcc catggcgcgg tcggcgtcgc gatccgagcg
     1261 cggcattcgc atgacccgga accgggacac atccaatgag actcgagaac aaggttgcga
     1321 tcgtcaccgg cgcggcgtcg ggctttggcg ccggcatcgc aaagcgcttc gccgcggaag
     1381 gggcgcgcgt cgtcgtcaac gatctgcatg ccgacggcgc aacgcgcatg gcggtggcga
     1441 tcgagcaggc gggcggccgt gccgttgcgt gcgcgggcga cgtatcgaag gatgccgacg
     1501 ttgcgcggct cgtcgcatcg gcgatcgacg cgttcggcag gctcgacgtc gtcgtcaaca
     1561 atgccggcac gacgcatcgc aaccagccga tgctcgacgt ccccgaggaa atgttcgacc
     1621 gcatctacgc ggtgaacgtg aaaagccttt acctcacggc acggcatgcg gtgccgcatt
     1681 ttcgggccca gcgcaacggt gcattcataa cgatcgcgtc gaccgccggc gtgcgcccgc
     1741 ggccggggct cacctggtac aacggcagca agggcgcggc gatcgtgacg tcgcgctcga
     1801 tggcggccga gcttgcgccg cacaacatcc gcgtcaacat catcaacccg gtcgccggag
     1861 aaacggccat gctcgccgat ttcatgggcg aggacacgcc gcagaagcgc gcgcagttca
     1921 tcgcgacgat tccgctcggg cgcctgtcgc agccctccga cgtcgcgacg gcggcggtgt
     1981 tcttcgcgtc cgacgaggcg gcgttcatca ccggcacgtg catggaagtc gacggcggcc
     2041 gctgcatttg aagtcggggt cagagccgta atattcgacg caacgcacgc gtctaatatt
     2101 acggctctga ccccgatttg cgccgcgcca cggccttcgc gttgaaaacc gactccgcgg
     2161 cgatgccgct gctgctcggc gcgctgatcg ctaccgcgcc gttcgcgatg gacgtctacc
     2221 tggcgtcgat gccgtcgatg acgcacgcgc tcggcgcgac gaccgccgag gttcagctca
     2281 cgctgtcggt gtacatgtac ggctggggcg tggcacagct tttcgcgggg ccgctgtccg
     2341 accgcttcgg acgccgcccc gtcctccttg cgagcctcgt cgtgttcgtg atcgcctcga
     2401 tcctctgtgc gctgtcgcgc aatgtgaccg tgctgatcgc cgggcggctg gtgcaggcgc
     2461 tgtcgatggc ttcgatcgca gtcgtgccac gggccgtcgt gcgcgatctc tacggcggcg
     2521 acaaggcggc gcacatcctg tcgctgatgg gggtcgtgct cggcatcgcc cccgtcgttg
     2581 cgccgctcat cggcagcaat ctgcacgtgt ggctcggctg gcagtcgaac ttcgtgctgg
     2641 tcgcgctcta cggcgccatt ctttggtgct gcgtgtacaa ggtgctgccc gagacgctcg
     2701 cgcgtcccga tgcgcgcgcc acggcgccgg ccgtgatcgc cgccaactac cggcggctgc
     2761 tgcgctcgcg cagctacgtc ggcttcatgc tggtcgccgc attcggattc agcggactgt
     2821 ttgcattcct ggccggcagc gcgttcgtgt tcgtatcggt gatgggcaag ggcgagcagg
     2881 gcttcggcgt catgttcggc accgtgatgc tcggcaacat cacaggcgcc accgtcggca
     2941 gccggctcgt gcggcgcatc ggcattgcgc gccagctccg cctcggtacc gcgctgatgc
     3001 tcgctgcagg attggcgatc ggggcgcccg cactgctcgg cgtcacgcat ccgctcgccg
     3061 tagtcgtgcc gatgttcgca ttcatggtga cgttcacgct gacgatgccg gccgccacgg
     3121 ccggtgcgct gacgccgttt ccgcaaattg ccggcagcgc ctcgtcgctg ctcgcgttct
     3181 gccagttcgt cgtcgcgtcg acggcggcgc tcgtcgtcgg gctcacgctc gatggcacca
     3241 cgcgtccgat ggcattgacg atcgccgtcg catcgcttgg cgcgttcgtg agcctgcgta
     3301 tcgcgcaccg cgaggcaata tagattgaat cagtcgatta cgcggcgccg gcgcagatcg
     3361 gcaatcgcgt cggcatcgta gccgaggtcg cgcagcacgg cgtcggtgtg ctcaccgagg
     3421 cgagggccgc cgccatcgaa gccgccgggt gtctgcgaca gcttcggcac gacaccgggc
     3481 acggcgagac gcgagccgtc gtggagtcgc acgtcgcgga tcatctcgcg cgccgcgaat
     3541 tgtgcgtcgg cgacgatgtc gcgaatcgtg tagatcttcg atgcgggcac gtcggccgtc
     3601 tgcatgacgg cgaccacctc ttccggcgcg cgacgcaccg tccattgctc gatctcaccg
     3661 tcgagccatt gctggcgcgc cgcgcgtccg tcgttgtgcg cgagcgacgg atcggacgcg
     3721 agatcgtcgc ggccgatcgc acggcaaagg cgctggaaga tggagtcgcc gttcgcggcg
     3781 atcagcacga agctcgcgtc cgcgcagcgg tacgccgacg tcggcgcaat gccggggagg
     3841 atcgatcccg tacgctcgcg cacggcgccg aagcgatcga attcgggcag catcgattcc
     3901 atcaccgaga aaaccgcttc gtacagcgcg acgtcgacga cctgaccgcg gccggtgctt
     3961 cgtcgtgatt cgagcgccat catcgcgccg atcacgccgt agagtgcgga cagcgtgtcg
     4021 ccgatcgaca cgccgacgcg cgacggtggc cggtcgggcg ttcccgtcac gtggcgcaga
     4081 cccccgacgg cttcgccgat cacgccgaag ccgggacgtt ctcgatacgg tcccgtctgg
     4141 ccataacccg agatgcgcac catgacgagc cccgggttcg cggcggcgag cgattcgtac
     4201 gccaggttcc agccttcgag cgtgcccggg cgaaaattct cgaccaggat gtcggcgcga
     4261 agcgcaagcg cgcgggcgat cgcctgaccg tcgggcgtgc gcaggtcgac cgccaccgag
     4321 cgcttgttgc gcgactgcac gtgccaccac agcgacgtgc cgttctcgac gtagcgccag
     4381 ttgcgcagcg gatcgccgct gccgggcggc tcgagcttca cgacgtcggc gccgaattcg
     4441 gcgagaatct tcgcgcagaa gggaccggcg atcagcgcac ccatttcgag gacgcgaatg
     4501 ccggcgagcg ggcggctcga tgccgcggat ggagtcacgt gccggcgtcc tcgcgttcga
     4561 tcgacgagcg ccgattatag tggcgatcac cgcagcagcc gtcgcgacgc gccagcgtgc
     4621 gagaatccgc cgcctcgatc cgatcgcctc cgatgcttgc gtccttcgac cgccgcctcc
     4681 tcgccgtcaa ccgcgtgctc gtcatcgtgc tgctcgcggc gatggcgatc atggtgttcg
     4741 cgaacgtcgc gctgcgcttc ctcaccgacc attcgatcct gtgggccgag gaggcgtcgc
     4801 gctacgcgat ggtgtggctc acgttcatcg gcgctggtcc ggtgctgcgt tacggcgggc
     4861 acatcggcat cgacacgctg gagcatgtgt cgccgcgcgc cgcacccgcc attcgtggcg
     4921 tcatcctgac gctgctcgcg gtgttcttct gcgtgatgat cgttgtcggc atccgctacg
     4981 cgagcctcac ctgggcgcag acgacgccgg tgctcgaagt tccgatcggg gccgtctatc
     5041 tcgcgatgcc gatcgggtcg gcactgctgc tcgtgcatct cgcgctgatg gcgcacggct
     5101 acgtcgcgcg gcgcgagctg ctcggcgacg gcgaattcga tgcggacgcc gcgaaactct
     5161 gacacgcgag cgcgcggatg gtgacgattc ttttcgtcgc ctgcgcgttg ctgctggcac
     5221 tcggcgttcc ggtcgccttc gcgctcggtg gcggcacgct cgccgcgatg ctgtggcagc
     5281 acgtgccgct cgtcgcgctg cccaagtaca tcttctccgg catcgatctc ttcgcgctcg
     5341 aagcggtgcc gttcttcatc ctgaccgccg agctgatgac cggcggtgag ctctccgcgg
     5401 tgctgctgcg cttcgcctcg cagttcgtcg ggcgctttcg cggcggcctc gggcacacga
     5461 acatcctgac gctgaccttc ttctccggca tctccggctc ggcgctcgcc gatgcggcgg
     5521 gtcccggcgc catcatgatc cgcatgatgc gagacgatgg ctacgacgcc ggctatgcgg
     5581 ccgcgctgac gtcggcgacg tcgattctcg gacccatcat cccgccgtcg atcagcgtca
     5641 tcatctacgc gctcgcgtat ccggacgtga acctggtggg cttgctgatc gccggcgtcg
     5701 tgcccggcat cctgatcgcc gcggcgatgg cggcgatcaa ccatgtgatt tcggtgcgcc
     5761 gcaactatcg cggcgccgcc gagcgcctcg ggtggcgcga gtacgtgcgc aacacctggc
     5821 gcgcgctgcc cgcgctggtg ctgcccgtgc tgatcctcgg cggcatcgcg tccggtgcgt
     5881 tcacgcccac cgaggcgtcg gtcgtcgccg tgttctacgc gctcttcgcc ggccgcgtca
     5941 tctatcggac actgcaatgg cggatggtgc cggcgattct cgcgcgctcg gcgctgatga
     6001 cggcgtcggt gcttttgatc gtcggcatgt cggcggcgtt cgcgtgggtg ctcacggtgt
     6061 cgcgcgtgcc gcaggacctg accgactgga tcgtcggcat gcagttgccg ccgctcgcat
     6121 tcctgctcgc cgtcaacgtg ctgctgctcg tgttcggcat cttcatcgag ccgctgcccg
     6181 gcatcgtcgt gctggtgccg atcctcgccc cgatcgcgca tgcgctccac atcgacgacc
     6241 tgcagttcgg catcgtcgtg atcgtcaacc tgacaatggg gatgatcacg ccaccggtgg
     6301 gtggattgct gttcgtgacg tcgatcgtcg cacgcgtgcc gctcgcgcgg ctcgtcaagg
     6361 agctgtggcc gtttctgtgg gcgcagctcg tcgtgctggc gctgctgtcg ctgtttccgg
     6421 tgctgtcgtc ggggctgccg aatttcttcg gctaccgctg atccggcgtc aggccggcac
     6481 gccgacgatc accgagaacg ggccggccgt cgcgacgacg cgatccaggc gaaatccggc
     6541 agcttcgaac aacgcctgat attcgcgctg cgtgcgttcg cggccgccgg tcagcgccag
     6601 catgacgatg tcgagcacct tggcgtgatg cgcttcgcca ccttccggaa gcggcgattc
     6661 gagcacgaga acttccgcat ggcgcggcgc ggagcgcctc acggcgcgca ggatgcgctc
     6721 cgcttcgcgg tcggcccaat cgtgcaggac gttgctcagc aggtaggcgt cggcttcggg
     6781 cagcgggtcc ttgaagaaat cgccgccgcg aaacgtcatc cgcgcggagg gcgcaagcgc
     6841 gccgacgacg gacggctgat cgaacagtac gccctgcgca cccggcgccg catcgaggac
     6901 ggcgcgcagg acgtgcccgc gcccgccgcc cacgtcggcg atcacgccat agcgcgagaa
     6961 atcgaacgcc ggcagaagcg cgcggatctc cgcgaccgct ttccccgtca ttgccgcgtc
     7021 gaagatccgc gcttcctccg ggtgatcgcg gaagtacgcc cacaatccgc cgcttacgag
     7081 cttctcgatc gcggcctcgc cggtgcgccc cgcgtactcg agttcccccg cggccgtcca
     7141 ttgcatgcga ccgccgatca tgcgcacgaa cgggcgcatc gattgcggat ggtcgctgcg
     7201 cagcagccgc gacagttcgg tgtgcgtcca caaggtcgcg cgcgcttcga aaatgcccgc
     7261 cacggcgagc agccgcagca ttcgctcgag cgcatccgcg ttcgcgccgg ctgcagcggc
     7321 gagcgcttcc gccgttgccg gtgtgtcgcc aagataatcg gcgacgccga gctccgcgac
     7381 gacatgcaga cagcgcggca ggaaatggcc ggtggcgagc tgccacaggc gcgccgaagg
     7441 cggtattgcc gtcattgcca ttcgcctttc gaggtcgtgc cgacgcccgg cgcgagcagc
     7501 ggctacttgt agttgcggat ccggtcgatg ttctcctgcc cgaatttctt cccgaattcg
     7561 gcgaacgtcg gcgccagcgc cgcct
//
LOCUS       DASSVX010000017        25203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1633662, whole genome shotgun sequence.
ACCESSION   DASSVX010000017 DASSVX010000000
VERSION     DASSVX010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 25203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25203
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633662"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2510)
                     /locus_tag="VFO53_00800"
     CDS             complement(<1..2510)
                     /locus_tag="VFO53_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET8874838.1"
                     /translation="MTDLSTHQTLVYTPRSKVRFVTAASLFDGHDASINIMRRILQAS
                     GCEVIHLGHNRSVEEIVTCAIEEDAHGIAVSSYQGGHVEFFTYMLDLLRERGAGHIKV
                     FGGGGGVIVPAEIEALHAYGVTRIFSPEDGQKLGLQGMINEIVAAADVDLTQRMPASV
                     DDLRTGDRFARAGALARAITALEAGVAPPAFREAIRAAAAHAKTPVLGITGTGGAGKS
                     SLTDELVRRFRLDQRDCTRIAILSIDPSRRKSGGALLGDRIRMNAIGTPDIYMRSLAT
                     RGAGSEISNALPDAIAACRTAGFDLVVVETSGIGQGDAAIVPHVDVSVYVMTPEFGAA
                     SQLEKIDMLDFADFVAINKFDRKGGEDALRDVRKQYQRNRSLFSVAPDAMPVYGTIAS
                     RFNDDGVTALYQAIAARLAEHGLALDKGALAPAPTRVSTQRQTIIPPPRTRYLAEIAE
                     TVRGYHDRALRQSRIARERQQLRAVKAMLQTKAGAETTSGEASPKVVVDPASAAALDT
                     LIAEREAALDVRARKLLDMWPRTKAAYAGDEYVVKIRDREIRTALNTTSLSGTKVRKV
                     ALPRYEDDGEILRWQLTENVPGSFPFTAGVFAFKRENEDPTRMFAGEGDAFRTNRRFH
                     LVADGMPAKRLSTAFDSVTLYGNDPDKRPDIYGKVGNSGVSIATLDDMKVLYSGFDLC
                     APNTSVSMTINGPAPTILAFFLNTAIDQKLTDFRDEHGREPSDAEAAELREWVLRNVR
                     GTVQADILKEDQGQNTCIFSTEFSLKVMGDIQEYFIDHDVKNFYSVSISGYHIAEAGA
                     NPISQLAFTLANGFTFVEAYLARGMRIDDFAPNLSFFFS"
     gene            2813..5533
                     /locus_tag="VFO53_00805"
     CDS             2813..5533
                     /locus_tag="VFO53_00805"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET8874839.1"
                     /translation="MILPTIRRFVLLLALLPATVVAALPAGVTQGPSVEGVDEYRLSN
                     GLTILLFPDATKPTTTVDVTYKVGSRMEDYGETGMAHLLEHMLFKGTPSVPSVFAELG
                     RRGMEFNGNTSYDRTTYFETFTASAASLDWVLMMESERMTRSTFSKADLDSEMTVVRN
                     EYESGENKPQQVLWKRMAGTAFDWHNYGKPTIGARSDIENVPFEKLRAFYAKYYQPDN
                     AVLTVAGKFDRDATLAAIAKSFGAIAKPTRVLPKFYTVEPVQDGERIVTVRRVASAQW
                     IGALFHLPQGANPDATAFEALAEIMTVEPAGRLYKALVEGKKASSVENWMFALHDAGF
                     VIFWAQVPPDQSIDAARDTMLATLYDVAAHPVTEAELERVKIKAQKDFDDTINDPQQM
                     AVALADSIAEGDWRLFFIHRDRWRELKPADVTRVATEYLKASNLTLGLFLPEAKPDRA
                     PLAAAVNIPALVRNYKGEPAVAAGEAFDPTPANLEARTQRFALSNGMKVALLPKKTRG
                     ETAQLEIRLDVGDVKSLANSTPVSSLTAEMLDRGTTKHDRQAFQDALDKLRAKLDIGG
                     GGATVSVGATTVRMSVPDVLRLAAEALREPAFSPSEFAQLKSELLTRLEQARTDPSSI
                     ARRAAARAGNPYPPDDVRYTPTIDEEIARVRATGLEAVKAFYAKFYGASHAEMAIVGD
                     FDVATVRPLLEQLFGGFRSATAYERVPQPFYPTKTAPMTFDTPDKANAAMFGRLSMPL
                     TDQAPDYASLLVANRVLGGDSDSRMFQRVRVKGGLSYSVGTVLQPASIDPNSTLVVYA
                     IFAPQNLRNVQSATAEEIARARDAGFNAQEIGAATKALLEERRITRAQDSALTGALVG
                     QEFLGRTWAESERIDAAIAAVTPESATVALRKYVIPADIAFAYAGDFAKK"
     gene            complement(5544..6332)
                     /locus_tag="VFO53_00810"
     CDS             complement(5544..6332)
                     /locus_tag="VFO53_00810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET8874840.1"
                     /translation="MASDERYAFAQAVARHAGALALEYWQNRERLTVELKGPQDFVSR
                     ADRDVEALIHRELGTAFPDDRFLGEETAAQFTGPIDRCWVVDPIDGTHNFLRGIAYWN
                     VAIAYVEEGEAQIGVVFDPVSGKLYHAARGAGAWCDDAPGTARLQVAATHELAGAYVA
                     LGHHDRAPDARYLEIRRRMMESGVAMRNFGSAALQLAHVAAGHLDGFVELQISVWDAI
                     GGLVLVDEAGGHVAPFKPATPTAKAACLACAPGIAPGLRAIVGI"
     gene            complement(6339..7361)
                     /locus_tag="VFO53_00815"
     CDS             complement(6339..7361)
                     /locus_tag="VFO53_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8874841.1"
                     /translation="MTNPRLRIGIAGLGRMGRRHAENLAHKVPQSELVAACSPVPDEL
                     EWAKHELGVAQCYTDYTQMLARAEIDAVFIVTPTTLHAQQIVEALRARKHVFSEKPLS
                     LDVAECRRVEAEAALSPDLKVMIGFVRRFDASYRHAHAYVRDGRIGRPLMVRSHTLDQ
                     DDPGGFFVRYSQASGGIFVDMSVHDIDAARWLLGVSDVARVFSTGLVGVHEGLREWND
                     VDNGVATCEFVTGEIATIHASRTMAHGFECITEVIGTKGRITVGGDARRDHVVVSDVH
                     GVRHECTPTFYERFEDAFLREDSEFVQAVIEDRPPPLTLRDATEATRIALAMRQSLLE
                     RRIVSL"
     gene            complement(7372..8358)
                     /gene="iolG"
                     /locus_tag="VFO53_00820"
     CDS             complement(7372..8358)
                     /gene="iolG"
                     /locus_tag="VFO53_00820"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HET8874842.1"
                     /translation="MIDVALFGAGRIGNIHAGNVARQPGVRLHHVVDVDRAAAQALAQ
                     QYGAEVPDAERAFADPAIAAVVIASSTDTHADLILRAAKAKKAIFCEKPVDLSLERAR
                     VCAEAVRAAGVPCMIGFQRRYDPTFASLKARIDEGEIGEPEMLVVTSRDPGAPPVDYI
                     KRSGDIFKDMLIHDFDIFRWILGDEAASVYATGSCLTDPAIEGAGDIDATAVTIRTRR
                     GRLAQINTIRRAAYGYDQRFEVIGARGMLQAGNHRPTEVVLWSSGAITADKPEHFFLE
                     RYRAAYAAEMAHFFDVVGGRARPRTSIDDGVAALALAEAATTSWREKRIVEL"
     gene            complement(8355..9164)
                     /gene="iolB"
                     /locus_tag="VFO53_00825"
     CDS             complement(8355..9164)
                     /gene="iolB"
                     /locus_tag="VFO53_00825"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HET8874843.1"
                     /translation="MSLIVKARREGRDIVRVTPQSAGWRYVGFSAHRLRDGETLAIDQ
                     PRDELCIVVLGGIVGVRQHDGARWPRIGARRDVFDDAAPYAVYLPQGRDVDVVAHGDA
                     QIAVASAPGPGNAPARLIEPAQMQRSVRGSGLNTRYVCDILPQSEPASHLLVVEVRTP
                     GGHSSSYPPHKHDSDNLPLESALEETYYHRLNPAQGFAFQRVYTDDRALDEALAVEDH
                     DVVMVPRGYHPVVVPYGYESYYLNVMAGPTREWHFRNDPAHEWMLAPGAIK"
     gene            complement(9161..10105)
                     /gene="iolE"
                     /locus_tag="VFO53_00830"
     CDS             complement(9161..10105)
                     /gene="iolE"
                     /locus_tag="VFO53_00830"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HET8874844.1"
                     /translation="MTGAGQRTAREPRELDVRIGINPISWSNDDLPSLGGETPLEVAL
                     TEGAAIGYEGFELGNKFPREPQALRGVLEPHGLALVSGWYSGRLAQRSADDEIAAVGS
                     HLTLLSEGGAKVMVYGEVADSIQGASVPLYKRPRFTHAGQWREYGRRVSEFARHTLER
                     GVRLAYHHHMGAYVQAPEDVDRLMENTGDEVGLLFDSGHMTFAGGDAVAMLERHVARV
                     CHVHCKDVRPQVIGLARNRDWSFLEAVINGAFTVPGDGAVDFSALIDILRRNGYRGWL
                     VVEAEQDPAVAPAYRYAQMGYRHLSTLVGAALREANRS"
     gene            complement(10200..12086)
                     /gene="iolD"
                     /locus_tag="VFO53_00835"
     CDS             complement(10200..12086)
                     /gene="iolD"
                     /locus_tag="VFO53_00835"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HET8874845.1"
                     /translation="MTAGVSGKSSLTPLSVRLTMAQALVRYLAALRVDADDARTRLFA
                     GVWAIFGHGNVAGVGEALFAHRDVLPTYRAHNEQAMAHAAIAFAKAHMRRRMMACTTS
                     IGPGATNLVTAAALAHANRLPVLFLPGDIFVSRAPDPVLQQIEDFQDGTVSANDCFKP
                     VSRYFDRITHPAQLLTALPRAIRVLTDPAACGPVTLALPQDVQAMAYDYPANFFEPPP
                     VRFDAMPPIASQIDAAVALLRASKRPLIVAGGGVLYGRASGALRSFADRHGVPVAETQ
                     GGKGALPWDDPMQQGSIGVTGSPAANGLAQEADVVIALGSRLQDFTTGSHSMFARAQL
                     VSINVAAFDAHKWRGLAVRADAGETLVALSRALDAWRAEPAWTSRAVEGGRAWRADIA
                     RITGRRDIALPYEGDVIGAVQRSAADSTTHDIVVCAAGSLPGELHKLWRTATPGGYHV
                     EYGYSCMGYEIAGGLGVKMAHPEREVVVMVGDGSYLMLNSEIATSVMLGLKLVVVVLD
                     NRGFGCINRLQQACGGAPFNNLLDDCIRAAPEPPKVDFAAHAMALGARAENVVDIAGL
                     EQALARARASDRTYVIAIETDPDRVTEEGGWWWDVAVPEVSPREKVRKARARYEQDRK
                     EQRP"
     gene            complement(12151..14118)
                     /gene="iolC"
                     /locus_tag="VFO53_00840"
     CDS             complement(12151..14118)
                     /gene="iolC"
                     /locus_tag="VFO53_00840"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HET8874846.1"
                     /translation="MHNNTRFAAGRRLDIVCLGRFGVDFYAQQIGARLEDVASFAKYL
                     GGSSANTAFGCARLGLRAGLVSRVGDDAMGRFLTETIAREGCDVSHVGVDPSRLTAAV
                     VLGIEDKDTFPLIFLRENCADMAIGDAEIDEAYIATSRALLITGTHFSTEYIAGISNR
                     ALDHAHAHDVRTVLDIDYRPVLWGLTKRGDGATRYIASETVTAHLQRMLPRFDLVIGT
                     IEEFNIAGGSDDIMASLRNVRGCTKAVLVVKRGPIGCAVIDGAIPESLDAAFNGTGVR
                     VEVLNVLGAGDAFSAGFMSGWVRGEDYDACVRYANACGALVVSRHGCAPAMPTRVELD
                     YFLAHAERIPRPDRDAALTRLHRVTAPRTRRDEVLAFAFDHRNPFFALAQQAGADEAR
                     IPRLKQLFVDAVAQVEAARGLQGRIGMLCDDRYGQDALNAATGRGWWIGRPVELPGSN
                     PLVFDRGRSVGTTLVSWPLEQVVKCLVPFHPDDAIDNRLEQEAQIRTLYDAVQASGHE
                     LLLEIVPPKSLPRTPDTVQRAMKRLYNLGIYPEWWKLEPMDATQWSAIDALIAERDPY
                     CRGVLILGQSAGAGVLAKAFRDARGSAACRGFAVGRTIFEAPSARWLAGDIDDAALVH
                     AVRASFEVLIDAWQEARAPLTTAQSWSQCRA"
     gene            14344..15528
                     /locus_tag="VFO53_00845"
     CDS             14344..15528
                     /locus_tag="VFO53_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8874847.1"
                     /translation="MQISRRWSFRVAAVIAGALLAASGRAADPPAPGGTVTGDTIMLG
                     QTAAQSGPQAPIGASKWGLEAFIAAVNARGGVAGKKLQLTSYDDGYQPAQTTSLDKKL
                     VYEDRVFAIVGSIGSPTTAAVYKKLNEAEVPLISMGSGSPIFYEPGLKYVFPSWPIYT
                     TDGKTMAAFVKKHFAGQSTAVIYQDDGFGKPILEGIKSGLGKIDVEIPYVPSQVDFSS
                     AVIKLKSSGIKVVMLATIATSGAQILNQMASLDYHPTRILTSSACGYGGIFSAIASLE
                     GTYCTAFLPPPGSADAHWKEFADAMGKYAPGHPPDIYAAWGWLAGQVTYAGLAKIKGP
                     ITREAFVAQLETLRDFDTIAGKINYSPQRHEGSCCQLLWQAKGGKWDIVPDSAFNGSA
                     MK"
     gene            15541..16419
                     /locus_tag="VFO53_00850"
     CDS             15541..16419
                     /locus_tag="VFO53_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8874848.1"
                     /translation="MTLYLLQICLAGLAQGSLFALAALGLVVIFNASTVVNFAHGAMG
                     MAATYVAYVLITSFHVPFALAFAGAIVAAFVLGVVVQKLLLGRLQGAPVLTQIVVTLG
                     LFMFLEGILGLAFGYNPRPMAAPVTMRPVILGGLVLRPQDVVQLGVLLVLCVALAALF
                     GATKLGLGMRAITENPYAARLMGIPVGSVLATAWGIGVLLAAVAAMLAAPTTSVTPNM
                     MDTIVVYAFVAAILGGFGSLIGALIGGLVLGVVVNLITAFLAPELAMSIVFGLLLLVL
                     YVKPDGLFGRASVQKV"
     gene            16428..17411
                     /locus_tag="VFO53_00855"
     CDS             16428..17411
                     /locus_tag="VFO53_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8874849.1"
                     /translation="MSRNTLVILLVIACALLPLVLQVMGQRQVYWLFVVSELFILAIV
                     AVSLDLLMGRSGQISLGHSGFVALGAYSSAILNERFGVDLFVGALAGGSLAALASLVL
                     GFPATRLRGHYLGIVTLGFGIAVAQIALKWTSLTGGDQGIHLHKPVLLGLDVATPVRM
                     YYVALVALVVVVLLVWRLTGTRVGRAFAAIKDSEVAAAAMGVPVARTKVLAFICSAFL
                     AGTAGGIYAFLAGFVAPEDFGIDSSLLYFAMVIIGGMGSIPGAIGGAVVVDVVKNAAA
                     TVSGLSLAILGGMIVLVVLFFPGGLKALLRRHPIVKTSTAERRGVAVAQDA"
     gene            17404..18180
                     /locus_tag="VFO53_00860"
     CDS             17404..18180
                     /locus_tag="VFO53_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8874850.1"
                     /translation="MLEARQVTVRFGGLVAVSELDLVVPDTGVFALVGPNGAGKTTLI
                     NALSRVCSVAEGTIRLDGVDVLGLLPHQAVRAGIARSFQRAELFPTLTVLENLLVGLH
                     AQMTTGLEGALMTPATRAQEKSARSRAERLLRELDLWELRNASPADLPYGHQKLLDVA
                     RAVISEPRLLLLDEPFAGLTDAESPRLLACIAAAGKRCAVLMIEHHFELLEGIALRMT
                     VMNFGRKIAEGSPAQVRADPEVVRCYLGTGRPKVAEAATC"
     gene            18174..18866
                     /locus_tag="VFO53_00865"
     CDS             18174..18866
                     /locus_tag="VFO53_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8874851.1"
                     /translation="MLKLDDVHVHYGRVHVLRGISLEVPDGAVVALLGANGAGKTTTL
                     KTISGILKPSRGSVHFDGEDISRLDAADIVRRGIIHCPEGRQVFPQLSVRENLMLGSY
                     ARGNARVQFERVLDIFPALRPRLAQLAGTLSGGEQQMLAIGRALMGEPKLLLLDEPSL
                     GLAPLIVEYIFQVIAKFREHDISVLLIEQNAMLALEFAGWAYALSHGQITFSHATQAL
                     RDTELVRRAYLA"
     gene            18922..20082
                     /locus_tag="VFO53_00870"
     CDS             18922..20082
                     /locus_tag="VFO53_00870"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET8874852.1"
                     /translation="MIRNSWYVAGLSRNFKYKLEKKIITAMPIVMWRAQDGNVVAFDG
                     RCCHKRFPLWDGKLLDNGVLRCAYHGLCYDSSGKCIDIPMQRDIPISPVARLRRYPVV
                     EQDGLVWLWPGDPEKIGNVKAPRIPELADPRYEAVISDPPIRIRANYRLLIENLLDIT
                     HFFPLHDGNIGDLANSMIPVGLVEDTVDGNPKVMTTRSVTNYRLPPYYQRWFGLDIVD
                     RDHTHAMTGPGLVRVQLRLAPPGKLGTDDECGYILCHTDTPIDEQHLEWHWIMISKAG
                     SRFPPDPSMTLAQGMAFEFPEVVAQDEWALAKQQEMLEFSDAFPDATRYREINMRSDI
                     GVVHARRVLSRMEKAENNEPFGLPSASLEMAKYAAAPGAQGAAAPETHGAVQ"
     gene            20079..20270
                     /locus_tag="VFO53_00875"
     CDS             20079..20270
                     /locus_tag="VFO53_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017599797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET8874853.1"
                     /translation="MKVVVDFDLCMGNAQCMLAAPQVFEVRDDGMLYLLQEEPPEALR
                     AAVENAARLCPTQAITIEK"
     gene            20274..21461
                     /locus_tag="VFO53_00880"
     CDS             20274..21461
                     /locus_tag="VFO53_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018089752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8874854.1"
                     /translation="MRSVVIVGASLAGLSAAKALRRQGFDGTVTLIGDEPHRPYQRPP
                     LSKQLLGGAWDRARLDLPDRGDLCLDWILGTAAASLDLATREVTLDDGRRVRFDGLVI
                     ATGARARKPRWHAELRGVFTLRTVEDALALRAHVAAGHRNVAIVGAGFIGCETAATLR
                     SSGCDVTLIDCERLPMLRALGESLGAMCRELHEAHGVRTVFGAGVEALLGRESVEGVR
                     LSDASIVDADCVVVGAGVTPNVEWLAGSGLVVDDGVVCDSTCAALGSEAVVAAGDVAR
                     WEHPHYGLRRIEHWNNAIAQADAAAGTLLAMPRRGVPYAAVPFFWSDQYDCKLQLIGA
                     PAAGDATRVVEGALGERRFVMLFEREGRVSGAFLFNSMHRVAAYTTAVEGAVGCVAEG
                     VGS"
     gene            21524..22720
                     /locus_tag="VFO53_00885"
     CDS             21524..22720
                     /locus_tag="VFO53_00885"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8874855.1"
                     /translation="MTAAETPPPLLAGLRVLEFGHIAAAPFCGMLLADLGADVVKVEA
                     PHGDGLRAWPPIVETPDGDSFSLNFASLNRGKRSVVADLKEAAELARIRDLCAHADVI
                     IENYRPGALDRLGIGFEDIATLQPTIVYCSISGYGHQGANATRGAFDVVVQGASGLMS
                     VTGEPGRPAVKCGVPVADFTAGLYAAYTIVAARGQALRERRAIHLDCAMLDCLLGISA
                     LQTSEYWGTGTAPKRLGSAHPRNAPYQAFDASDGQFILAAGNDALWREVCEATGQHAL
                     LDDPRFRGISLRAKNQAALAALLQQVFRTRTVREWLDEFERRGVPSGPLNTFADILGD
                     PELTASELVQDMRVPVAGTTKTVAYPVRLNGHRMRAQRPPPRLGEHRDQVFAEWLGAS
                     ASSERA"
     gene            22717..23484
                     /locus_tag="VFO53_00890"
     CDS             22717..23484
                     /locus_tag="VFO53_00890"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8874856.1"
                     /translation="MRGALRVEQDAPRAGVWRLVLDDAPHANALSPALVGRLQAALHA
                     AFERDVRCIVIASSSERFCAGADLADAHHLDDSALRDRFSALEDLLETVRRAPALTIA
                     VVRGAALGAGADLVTSCDYRLGTPKARLAFPGSRFGVVLGTRHLAAVIGRQAARELLV
                     EGKSLDAQAAAQCGLLSELCAEDAIDSRVESIAQSSEAIDAPTLRAILRLTRELPSER
                     DRAELLQSTSRNGLAERLREHARSAQEQRALRRAAGQ"
     gene            23607..24332
                     /locus_tag="VFO53_00895"
     CDS             23607..24332
                     /locus_tag="VFO53_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET8874857.1"
                     /translation="MAKALLVLECIGLAKRRTLTEIAHEVTLPKSTLLRLIQTLIARG
                     FVRRTAHGEYAVSLKLWQIGCNALDSEAIRDEVIPLLNDLVGATGETAHYAAYEGGFS
                     VYIEKVDGLHPIRSYTAVGGRSPAYATATGKALLAWRDEREIAKVGRSARRWTDATQA
                     GADEVLREAEQTRRVGYAVNRGEWRASVWGIGAPVFDRHAQVIAAVGISGPRERIEPN
                     VRPYAKLVVAAAKALSRRMGSPG"
     gene            complement(24459..24944)
                     /locus_tag="VFO53_00900"
     CDS             complement(24459..24944)
                     /locus_tag="VFO53_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874858.1"
                     /translation="MARSTLNTTGEGKGGTQKGHGTGALGPSDSTDSGSDIVGGPGLN
                     RDGGLPLQRGTTSDPDVDSAPTTAGPDLGDADLDSDSDRYGTGERAAAGRDSTEAVDE
                     VLTEHDADETGSMNVMDDSLTEREADSVESDDNPEAEVEAIEAIEKRPGINGAVRRSR
                     K"
BASE COUNT         4019 a         8569 c         8462 g         4153 t
ORIGIN      
        1 gagaagaaga aggaaagatt gggcgcgaag tcgtcgatcc gcatgccgcg cgcgagatag
       61 gcctcgacga acgtgaatcc attggccagg gtgaaggcca attgcgagat cgggttcgca
      121 cccgcctcgg cgatgtgata cccggaaatc gataccgagt agaagttctt cacatcgtga
      181 tcgatgaagt actcctggat gtcgcccatc accttcagcg agaactcggt cgagaagatg
      241 caggtgttct gcccctggtc ttccttgagg atgtcggcct gcaccgtgcc gcggacgttg
      301 cgcagcaccc actcgcgcag ctcggcggcc tcggcgtcgg acggctcgcg gccgtgctcg
      361 tcgcggaagt ccgtcagctt ctggtcgatc gccgtgttga ggaagaacgc gaggatcgtc
      421 ggcgcgggac cgttgatcgt catcgacacc gacgtgttcg gcgcgcaaag gtcgaagccg
      481 gaatacagca ccttcatgtc gtcgagcgtc gcgatcgaca cgcccgagtt gccgaccttg
      541 ccgtagatgt ccggacgctt gtcggggtcg ttgccataga gcgtcaccga atcgaacgcg
      601 gtcgacaggc gcttggccgg catgccatcg gcgaccaggt ggaagcggcg gttcgtgcgg
      661 aacgcgtcgc cttcgccggc gaacatccgc gtcgggtcct cgttctcgcg cttgaacgcg
      721 aagacgcccg cggtgaacgg aaagctgccc ggcacgttct ccgtcaactg ccagcgcagg
      781 atctcgccgt cgtcttcgta gcgcggcagc gcgaccttgc gcaccttcgt gcccgacagc
      841 gacgtcgtgt tgagcgcggt gcggatctcg cggtcgcgga tcttcacgac gtattcgtcg
      901 ccggcgtacg cggccttcgt gcgcggccac atgtcgagaa gcttccgcgc gcgcacgtcg
      961 agcgccgctt cccgctctgc gatgagcgtg tcgagcgccg ccgccgaagc agggtcaacg
     1021 acgactttcg gcgacgcttc accggaagtc gtctccgccc ctgctttggt ttgcagcatc
     1081 gccttcacgg cccgcaactg ctgacgctcg cgcgcgatgc gtgactggcg gagcgcgcgg
     1141 tcgtggtagc cgcggaccgt ctcggcgatc tcggcaagat agcgcgtgcg cggcggcgga
     1201 atgatcgtct gccgctgcgt cgaaacgcgc gtcggcgctg gcgcaagcgc gcccttgtcg
     1261 agcgcgaggc cgtgctcggc gaggcgtgcc gcgatcgcct ggtagagcgc ggtgacgccg
     1321 tcgtcgttga agcgcgacgc gatcgtcccg tataccggca tcgcgtcggg cgcgacgctg
     1381 aagagcgaac ggttgcgctg gtactgcttg cgcacgtcgc gcagcgcatc ctccccgccc
     1441 ttgcggtcga acttgttgat ggcgacgaaa tccgcgaagt ccagcatgtc gatcttctcg
     1501 agctgcgacg ccgcgccgaa ctcgggtgtc atcacgtaga ccgacacgtc gacgtgcggc
     1561 acgatcgccg cgtcaccctg gccgatgccg gacgtctcga cgacgacgag gtcgaacccg
     1621 gccgtcctgc atgccgcgat cgcatccggc agcgcgttcg aaatctcgct gccggcgcca
     1681 cgcgtggcga gcgagcgcat gtagatgtcc ggcgtcccga tcgcattcat gcgaatgcgg
     1741 tcgccaagca gcgcgccgcc ggacttgcgc cgcgacggat cgatcgacag gatggcgatg
     1801 cgcgtgcaat cgcgctggtc gagccggaaa cggcgcacga gctcgtcggt caacgagctc
     1861 ttgccggcgc cgccggtgcc ggtgatgccg agcacgggcg tctttgcatg agcggccgcc
     1921 gcgcgtatcg cctcccggaa tgccggcggt gcaacgcccg cttcgagcgc ggtgatcgcg
     1981 cgggcgagcg cacctgcacg ggcaaagcgg tcgccggtgc gaaggtcgtc gacggacgcc
     2041 ggcatccgct gcgtcaggtc gacatcggcc gcggcgacga tttcgttgat catcccctgc
     2101 agcccgagct tctgcccgtc ctccggcgaa aagatccgcg tcacgccata cgcgtgcagc
     2161 gcctcgatct ccgcgggcac gatgacgcca ccgccgccgc cgaagacctt gatgtggccc
     2221 gcgccgcgct cgcgcaaaag atcgagcatg taagtgaaga actcgacatg gccgccctgg
     2281 tacgacgaga ccgcgatgcc gtgggcatcc tcctcgatcg cgcaggtgac gatctcctcg
     2341 accgaccggt tgtgaccgag atggatcacc tcgcagccgc tcgcctgcag gatgcgccgc
     2401 atgatgttga tcgacgcgtc gtggccgtcg aagaggctcg ccgccgtgac gaaacgcacc
     2461 ttcgaccgcg gcgtgtaaac gagcgtctgg tgcgtggaaa ggtcggtcat cgcgaacctc
     2521 gatgaaaagc gggtcgctgc ggggcgcgag gcgttgggaa agcttaccgg aagcgccgcg
     2581 cgcgcggctc gagcccggcg ccggttgcgc ccgtggcgac ccggtgtcgg ggcagacgct
     2641 acatgacgtt tacgtaaacg tcaatccagc gccggacggt aggccacgga aaacccgagg
     2701 cgcacggcgc cgggcgtccg gagccgcatg cgctacgctt cggcactttt ggcgcccagc
     2761 cgctgtccca tcgacgcatc ctgcgccctc cgccgccttc cctacccagc ccatgatcct
     2821 gccgacgatc cgccggttcg ttctccttct tgccttgctt cccgcgaccg tcgtcgcggc
     2881 gctgcctgcg ggcgtcacgc aggggcccag cgtcgagggt gtcgacgagt acaggctgtc
     2941 gaacggactc accatcctgc tgttccccga cgcgaccaag cccacgacga ccgtcgacgt
     3001 cacctacaag gtcggctcgc gcatggagga ctacggcgag acgggcatgg cgcacctgct
     3061 ggagcacatg ctgttcaagg gcacgccgtc ggtgccgagc gtgttcgccg agctcggccg
     3121 gcgcggcatg gaattcaacg gcaacacgtc gtacgaccgc acgacctatt tcgagacgtt
     3181 caccgcgtcg gcggcatcgc tcgactgggt gctgatgatg gagtccgagc gcatgacgcg
     3241 ctcgacgttc agcaaggcgg atctcgactc cgagatgacc gtcgtgcgca acgagtacga
     3301 aagcggcgag aacaagccgc agcaagtgct ctggaagcgg atggcgggaa cggcgttcga
     3361 ctggcacaac tacggcaagc cgacgatcgg ggcgcgctcc gacatcgaga acgtgccgtt
     3421 cgagaagctg cgcgcgttct acgcgaagta ttaccagccc gacaacgcgg tgctgaccgt
     3481 cgccggcaag ttcgatcgcg acgcgacgct cgccgccatc gcgaagtcgt tcggagcgat
     3541 cgcgaagccg acgcgcgtgt tgccgaagtt ctacacggtc gagccggtgc aggatggcga
     3601 gcgcatcgtg acggtgcgcc gcgtggcgag cgcgcaatgg atcggcgcgc tcttccacct
     3661 gccgcagggc gcgaatcccg acgcgacggc attcgaagca ctcgccgaga tcatgacggt
     3721 cgagccggcc ggacgcctct acaaggcgct cgtcgagggc aagaaggcgt cgtccgtcga
     3781 gaactggatg ttcgcgctgc acgacgcggg tttcgtgatc ttctgggcgc aggtgccgcc
     3841 cgaccagtcg atcgacgcgg cgcgcgacac gatgctcgcc acgctctacg acgtcgccgc
     3901 gcatccggtc acggaggccg agctcgagcg ggtgaagatc aaggcgcaga aggatttcga
     3961 cgacacgatc aacgacccgc aacagatggc ggtggcgctt gccgattcga tcgccgaggg
     4021 cgactggcgg ctcttcttca tccaccgcga tcgctggcgc gaattgaagc cggccgatgt
     4081 cacgcgcgtc gccaccgaat acctgaaagc gtcgaacctg acgctgggcc tgttcctgcc
     4141 cgaggcgaaa cccgatcgcg cgccgctcgc cgctgccgtc aacatcccgg cgctggtgcg
     4201 aaattacaag ggcgagccgg ccgttgcggc gggcgaagca ttcgatccta cgcccgccaa
     4261 cctcgaggcg cgcacgcagc gcttcgcgct gtcgaatggg atgaaggtcg cgcttttgcc
     4321 gaagaagacg cgcggcgaaa cggcacagct cgaaatccgc ctcgacgtcg gcgacgtcaa
     4381 gtcgctcgcg aacagcacgc cggtgtcgag cctcaccgcc gagatgctcg atcgcggcac
     4441 cacgaagcac gatcggcagg cgttccagga tgcgctcgac aagctccgcg cgaagctcga
     4501 catcggcggc ggcggcgcaa ccgtgagcgt cggcgcaacc accgtgcgca tgagcgtacc
     4561 cgacgtgttg cggctcgctg ccgaagcact gcgcgagccg gcgttctccc cgtccgaatt
     4621 cgcgcaactg aagagcgagt tgttgacccg gctcgagcag gcgcgcaccg acccgagctc
     4681 gatcgcccgc cgcgccgccg cccgcgcagg caatccttat cctcccgacg acgtccgcta
     4741 cacgccgacg atcgacgagg aaatcgcacg cgtacgcgcc accggcctcg aggcagtgaa
     4801 ggcgttctac gcgaagttct acggcgcttc gcatgccgaa atggcgatcg tcggcgattt
     4861 cgacgtggca acggtccggc cgctcctcga gcagctcttc ggcggattca ggagcgcgac
     4921 ggcctacgag cgcgtgccgc agcctttcta tccgacgaag acggcgccaa tgacgttcga
     4981 cacgcccgac aaggcgaacg cggcgatgtt cgggcgcctt tcgatgccgc tcacggatca
     5041 ggcgcccgac tatgcatcgc tgctcgttgc caatcgcgtg ctcggcggtg acagcgattc
     5101 gcggatgttc cagcgcgtgc gagtcaaggg cggcctctcg tactcggtcg gcaccgtgct
     5161 ccagcccgcg tcgatcgatc cgaacagcac gctcgtcgtc tatgcgatct tcgcgccgca
     5221 gaatctgcgc aacgtgcaaa gcgccaccgc cgaggaaatc gcccgcgccc gcgacgccgg
     5281 tttcaacgcg caggagatcg gcgcggccac gaaggcgctg ctcgaggagc gccgcatcac
     5341 gcgtgcgcag gactcggcac ttaccggcgc gctcgtcggc caggagttcc tcggccgcac
     5401 gtgggccgaa tcggagcgga tcgacgcggc gatcgccgcg gtgacacccg agtcggcgac
     5461 cgttgcgttg cgcaaatacg tgatcccggc cgatatcgcg ttcgcgtacg cgggcgactt
     5521 cgcgaagaag tagcgggcgg cagctaaatt ccgacgatcg cgcggaggcc cggcgcaatg
     5581 cccggcgcgc aggcaaggca ggccgccttg gcggtgggcg tggccggctt gaacggcgcc
     5641 acgtggccgc ccgcctcgtc gacgagcacc aatcccccga tcgcgtccca gaccgaaatc
     5701 tgcagctcga cgaagccgtc gagatgcccg gcggccacgt gcgcgagctg cagcgccgcc
     5761 gagccgaagt tgcgcatcgc gacgccgctt tccatcatcc gccgccggat ctcgagatag
     5821 cgcgcatcgg gtgcgcgatc gtgatggccg agcgccacgt acgcgcccgc gagctcgtgc
     5881 gttgcagcga cctgcagccg cgccgtgccc ggcgcgtcat cgcaccatgc gccggcgccg
     5941 cgggcggcgt ggtacagctt cccgctgacc ggatcgaaca ccacgccgat ctgcgcctcg
     6001 ccctcttcga catacgcgat tgcgacgttc cagtaggcga tgccgcgcag gaagttgtgc
     6061 gtgccgtcga tgggatcgac cacccagcag cgatcgatcg ggccggtgaa ctgcgctgcg
     6121 gtttcctcgc cgaggaagcg atcgtcggga aacgcagtgc cgagttcccg gtgaatcaac
     6181 gcctcgacgt cccggtcggc acggctcacg aagtcctgcg gacctttgag ctcgacggtc
     6241 agccgctcgc ggttctgcca gtactcgagc gccagcgcac ccgcatgccg ggcgacggcc
     6301 tgcgcgaatg cgtagcgctc gtcgctagcc aaatcggcct acagcgacac gatgcgccgc
     6361 tcgagcagcg actggcgcat tgccagggcg atgcgggtcg cctcggttgc gtcgcgcagc
     6421 gtgagcggcg gtggccgatc ctcgatcacc gcctgcacga attcgctgtc ttcgcgcagg
     6481 aaggcatcct cgaagcgctc gtagaaggtc ggcgtgcatt catgccgcac accgtggaca
     6541 tcggagacca cgacatggtc gcgccgcgcg tcgccgccga cggtgatgcg tcccttggtg
     6601 ccgatcactt cggtgatgca ctcgaagccg tgcgccatcg tgcgcgacgc gtggatggtc
     6661 gcgatctcgc cggtgacgaa ttcgcaggtc gcgacgccgt tgtccacgtc gttccattcg
     6721 cgcaacccct catgcacgcc aacgagaccg gtcgagaaca cgcgcgccac gtcggacacg
     6781 ccaagcagcc agcgcgcggc gtcgatgtcg tgcacgctca tgtcgacgaa gatgccgccg
     6841 ctcgcctgcg aatagcggac gaagaagccg ccgggatcgt cctggtcgag cgtgtgcgag
     6901 cgcaccatca gcggccggcc gatgcgcccg tcgcggacat acgcgtgcgc gtggcgatag
     6961 cttgcatcga agcgccgcac gaagccgatc atcaccttga ggtccggcga gagcgccgct
     7021 tcggcctcga cgcggcggca ctcggcgacg tcgagcgaca gcggtttttc ggagaacacg
     7081 tgcttgcggg cgcgcagcgc ctcgacgatc tgttgcgcat gcagcgtcgt cggcgtgacg
     7141 atgaacacgg cgtcgatttc tgcccgggca agcatctgcg tgtagtcggt gtagcactgc
     7201 gccacgccga gctcgtgctt cgcccattcg agctcgtcgg gcacgggact gcaggcggcg
     7261 acgagctcgc tctgcgggac cttgtgcgcg aggttctcgg cgtggcgccg gcccatgcgg
     7321 ccgaggccgg caatgccgat tcgcaatcgt ggattggtca tgtcggtgaa ttcaaagctc
     7381 gacgatgcgc ttctcgcgcc acgatgtcgt cgccgcttcc gccagtgcca gcgccgcgac
     7441 gccatcgtcg atcgacgtgc gcggcctcgc gcggccgccg accacgtcga agaaatgcgc
     7501 catctcggcc gcgtacgcgg cgcgatagcg ctcgaggaag aagtgttcgg gcttgtccgc
     7561 ggtgatcgcg ccggacgacc acagcacgac ttccgtcggg cggtggttac cggcctgcag
     7621 catgcccctg gcaccgatca cctcgaagcg ctggtcgtag ccgtacgccg cgcggcggat
     7681 cgtgttgatc tgcgcgaggc gtccgcgtcg cgtgcggatc gtcaccgcgg tcgcatcgat
     7741 gtcgcctgcg ccttcgatgg ccggatcggt gagacagcta cccgtcgcgt agacgctcgc
     7801 cgcctcgtcg ccgagaatcc agcggaagat gtcgaagtcg tggatcagca tgtccttgaa
     7861 gatgtcgccc gagcgcttga tgtaatccac cggcggcgcg ccggggtcac ggctcgtgac
     7921 gacgagcatt tcgggctcgc cgatctcgcc ctcgtcgatc cttgccttga gcgacgcgaa
     7981 cgtcggatcg tagcggcgtt ggaagccgat catgcacggc acccccgccg cccgaaccgc
     8041 ttcggcgcag acgcgtgcgc gctcgagcga cagatcgacg ggcttctcgc agaagatcgc
     8101 ctttttcgcc ttcgcggcgc gcaggatcag gtcggcatgc gtatcggtgg acgatgcgat
     8161 gacgaccgcg gcgatcgccg gatcggcaaa tgcgcgctcg gcatccggca cctcggcgcc
     8221 gtactgctgc gccagcgcct gcgccgcggc acggtcgacg tcgacgacat ggtgcagccg
     8281 gacgccgggc tggcgcgcaa cgttgccggc gtgaatgttg ccgatgcgtc cggctccgaa
     8341 cagtgcgacg tcgatcattt gatggctccg ggtgcaagca tccattcgtg ggcgggatcg
     8401 ttcctgaaat gccattcgcg cgtcgggccc gccatcacgt tcagatagta cgattcatag
     8461 ccgtacggca cgacgaccgg atgatagccg cgcggcacca tgacgacgtc gtggtcctcg
     8521 acggcgagcg cctcgtcgag tgcgcgatcg tcggtgtaca cgcgctggaa ggcgaagccc
     8581 tgcgcggggt tcagccgatg gtagtacgtc tcctcgagcg cgctctccag cggcaggttg
     8641 tccgagtcgt gcttgtgcgg cggatagctc gacgaatgcc cgcccggcgt gcgcacctcg
     8701 acgacgagca gatgcgacgc cggctcgctc tgcggcagga tgtcgcacac gtagcgcgtg
     8761 ttgagtccgc tgccgcgcac cgaacgctgc atctgcgcag gctcgatcag acgcgcgggc
     8821 gcattgcccg gtcccggcgc actcgcgact gcgatctgcg cgtcgccatg cgcaacgacg
     8881 tcgacgtcgc ggccttgcgg cagataaacg gcgtagggcg cggcgtcgtc gaacacgtcg
     8941 cggcgcgcac cgatccgcgg ccagcgtgcg ccgtcgtgct ggcgcacgcc gacgatgccg
     9001 ccgagcacga cgatgcaaag ctcgtctcgc ggctgatcga tggcgagtgt ttcgccatcg
     9061 cgaagccggt gcgcggaaaa gccgacgtag cgccagcccg ccgactgcgg cgtcacgcgc
     9121 acgatgtcgc gaccctcgcg tcgcgccttc acgatcaggc tcatgaccga ttcgcctccc
     9181 ggagagcggc gcccaccagc gtcgacagat ggcgatagcc catctgcgcg taacgatacg
     9241 cgggtgcgac cgcaggatcc tgctcggctt cgacgacgag ccatccgcga taaccattac
     9301 gccgcagaat gtcgatcaag gcggagaaat cgaccgctcc gtcgccgggc acggtgaacg
     9361 caccgttgat gacggcttcg agaaagctcc agtcgcgatt gcgggcaagc ccgatgacct
     9421 gtggccgcac gtccttgcag tggacgtgac acacgcgggc gacatggcgc tcgagcatcg
     9481 ccaccgcgtc gccgccggcg aacgtcatgt ggccgctgtc gaacagaagg cccacttcat
     9541 cgccggtgtt ttccatgagc cggtcgacat cctccggcgc ctgcacgtac gcacccatgt
     9601 gatggtgata cgcgagacga acgccgcgct cgagcgtgtg gcgtgcgaac tcgctcaccc
     9661 gcctgccgta ttcgcgccat tgacctgcat gcgtgaaccg cggccgcttg tacagcggca
     9721 cgctcgcccc ctgaatgctg tcggcgactt cgccgtacac catgaccttc gcgccgccct
     9781 cggagaggag cgtcaggtgc gaccccaccg cggcgatttc gtcgtcggcc gagcgctgcg
     9841 caaggcgccc cgaataccag ccggaaacga gcgccaggcc gtgcggctcg agcacgccac
     9901 gcagcgcctg cggctcgcgc ggaaacttgt tgccgagctc gaatccttcg tagccgatgg
     9961 ccgcgccttc ggtgagcgcc acctcgagcg gcgtctcgcc gccgagcgac ggcagatcgt
    10021 cgttgctcca ggaaatcgga ttgatgccga tccgcacatc cagttccctt ggctcgcgcg
    10081 ccgtgcgctg cccggcgccg gtcatggtcc ccccacgctt gcggcctcat ttgccaattc
    10141 ggccgctgcg ctgcccccgt tgggggtgca attcgcggct tgggacggcc ccgcgccgct
    10201 catggccgct gctccttccg gtcctgctcg tagcgtgcgc gcgccttgcg caccttctcg
    10261 cgcggcgaca cctcggggac cgccacgtcc caccaccagc cgccttcctc cgtgacgcga
    10321 tccggatcgg tctcgatcgc aatgacatac gtgcggtcgg atgcgcgcgc ccgcgcaagc
    10381 gcctgctcga gcccggcgat gtccacgacg ttttccgcac gcgcgccgag cgccatggcg
    10441 tgtgcggcga aatcgacctt cggcggctcc ggcgccgcgc ggatgcaatc gtcgagcagg
    10501 ttgttgaacg gcgcgccgcc gcacgcctgc tgcagccggt tgatgcagcc gaagccacga
    10561 ttgtcgagca cgacgacgac gagcttcagc ccgagcatca ccgatgtcgc gatttcggaa
    10621 ttcagcatca ggtagctgcc gtcgccgacc atcacgacga cctcgcgctc gggatgcgcc
    10681 attttgacgc cgagcccgcc cgcgatctcg tagcccatgc acgagtagcc gtactcgacg
    10741 tgatacccgc cgggcgtcgc cgtgcgccag agcttgtgca gttcaccggg cagcgagccc
    10801 gcggcgcaca cgacgatgtc gtgcgtcgtc gaatcggcgg ccgagcgctg taccgcgccg
    10861 atgacgtcgc cttcgtacgg caacgcgatg tcacgccggc cggtgatccg cgcgatgtcg
    10921 gcgcgccatg cgcggccgcc ttcgacagcg cgcgacgtcc atgccggttc cgcccgccag
    10981 gcgtccagcg cgcgggacaa cgcgacgagc gtctcgccgg catccgcgcg caccgcgagc
    11041 cctcgccatt tgtgcgcgtc gaatgccgcg acgttgatcg agacgagttg cgcacgcgcg
    11101 aacatcgagt gcgagccggt cgtgaaatcc tgcaggcgcg agccgagcgc gatcacgacg
    11161 tcggcctcct gcgcgagccc gttcgccgcc ggcgaaccgg tgacgccgat cgatccctgc
    11221 tgcatcgggt cgtcccacgg cagcgcgccc ttgccacctt gcgtttcggc caccggcacc
    11281 ccgtgccgat ccgcgaaact cctgagcgcg ccgctcgcgc gcccgtagag cacgccgccc
    11341 ccggcgacga tcagcggccg cttcgatgcg cgcagcaatg caacggcggc atcgatctgc
    11401 gatgcgatcg gcggcatggc gtcgaagcgc accggtggcg gctcgaaaaa atttgccggg
    11461 tagtcgtagg ccatcgcctg cacgtcctgc ggcagcgcaa gcgtcaccgg tccgcacgcg
    11521 gccggatcgg tcagcacgcg aatggcgcgc ggcagcgcgg tcagcaactg cgcaggatgg
    11581 gtgatgcgat cgaaatagcg cgagacgggc ttgaagcagt cgttggccga aacggtgccg
    11641 tcctggaaat cctcgatctg ctgcaacacc ggatcgggcg cgcgcgagac gaagatatcg
    11701 ccgggaagga acagcaccgg caggcggttc gcgtgagcaa gcgccgcggc ggtgacgaga
    11761 ttcgtcgccc ccgggccgat cgacgtcgtg cacgccatca tgcggcggcg catgtgcgcc
    11821 ttggcgaacg cgatcgccgc atgcgccatc gcctgctcgt tgtgcgcgcg gtaggtgggc
    11881 agcacgtcgc gatgggcgaa aagcgcctcg ccgacaccgg cgacgttgcc atggccgaag
    11941 atcgcccaca caccggcaaa gagccgggtc cgtgcgtcgt ccgcgtcgac gcgcagcgcc
    12001 gccaggtagc gcacgagcgc ctgcgccatc gtgagccgaa ccgaaagtgg ggtcagagac
    12061 gactttcccg atactcccgc ggtcatatcg agatgctccc tggaaagtcg tctctgaccc
    12121 cactttcaat cgtgagccga agggtgtcgt tcatgcgcgg cattgcgacc agctctgcgc
    12181 ggtggtcagc ggcgcgcgcg cctcctgcca tgcatcgatc agcacctcga agctcgcgcg
    12241 tacggcatgc acgagcgcgg catcgtcgat gtcgcccgca agccagcgtg ccgacggcgc
    12301 ttcgaagatc gtgcggccca cggcgaaacc gcggcacgcc gcactgccgc gcgcatcgcg
    12361 aaacgccttc gcgaggacgc cggcgcctgc gctctgcccg aggatcagca cgccgcggca
    12421 gtacggatcg cgctcggcga tcagcgcgtc gatggcgctc cactgtgtcg cgtccatcgg
    12481 ctcgagcttc caccattcgg ggtagatgcc caggttgtag agccgcttca tcgcgcgctg
    12541 caccgtgtcc ggcgtgcggg gcagcgactt cggcggcacg atttcgagca gcagctcgtg
    12601 gccgctcgcc tggaccgcgt cgtacagcgt gcggatctgc gcttcctgct cgaggcggtt
    12661 gtcgatcgcg tcgtccggat gaaacggcac gaggcacttg acgacctgct ccagcggcca
    12721 gctcacgagc gtggtgccga ccgagcgccc gcggtcgaac acgagcggat tcgagcccgg
    12781 caattcgacc ggccgtccga tccaccagcc gcggcccgtg gcagcattga gcgcatcctg
    12841 accgtaacgg tcgtcgcaca gcatcccgat gcggccctgc aggccgcggg ccgcctcgac
    12901 ctgcgccacg gcgtcgacga agagctgctt caaccgcggg atgcgcgcct catccgcgcc
    12961 tgcctgctgc gccagcgcga agaacgggtt gcgatggtcg aacgcgaacg cgagcacctc
    13021 gtcgcggcgc gtgcgcggcg cggtgacgcg gtgcaggcgc gtgagcgctg catcgcgatc
    13081 gggacgcggg atgcgctcgg catgggcaag aaagtagtcg agctcgacgc gcgtaggcat
    13141 cgccggcgca cagccgtgac gcgacacgac gagcgcgccg catgcgttcg catagcgcac
    13201 acaggcgtcg tagtcctcgc cgcgcaccca tcccgacatg aatcccgcgg agaacgcgtc
    13261 gcccgcaccg agcacgttca gcacctcgac gcggaccccg gtcccgttga atgccgcgtc
    13321 gagcgattcg ggaatcgcgc cgtcgatcac cgcgcagccg atcgggccgc gcttcacgac
    13381 gagcaccgcc ttcgtgcacc cgcgcacatt gcgaagcgat gccatgatgt cgtcgctgcc
    13441 gccggcgatg ttgaactcct cgatcgtgcc gatcacgaga tcgaagcgcg gcagcatccg
    13501 ctgcaggtgc gcggtgaccg tttccgaggc gatgtagcgc gtcgcgccgt cgccgcgctt
    13561 ggtgagtccc cacagcaccg gccggtagtc gatgtcgagg acggtgcgca cgtcgtgcgc
    13621 atgcgcgtgg tcgagcgcac ggttgctgat gccggcgatg tattcggtcg agaaatgcgt
    13681 gccggtgatt agcagcgcgc gcgacgttgc gatgtacgct tcgtcgatct cggcgtcgcc
    13741 gatcgccatg tcggcgcaat tctcgcgcag gaagatgagc gggaacgtgt ccttgtcctc
    13801 gatgccgagg accacggcgg ccgtgagccg cgacggatcg acgccgacgt ggctcacgtc
    13861 gcagccttcg cgggcgatcg tctcggtcag gaagcgcccc atcgcgtcgt cgccgacacg
    13921 cgagacgagg ccggcccgca ggccgagccg cgcgcacccg aaggcggtgt tcgccgacga
    13981 gcccccgaga tatttcgcga agctcgcgac gtcttcgagc cgcgcgccga tctgctgggc
    14041 gtagaaatcg acgccgaagc gtccgaggca gacgatgtcg agccggcggc cggccgcgaa
    14101 acgggtgttg ttgtgcatgc tcgacgtcgg tgcgcgctgc gcgatgggcg ttgcctcggc
    14161 cgccgatgat atcgcgttcc cgtgcgcggc gacgcgtgag ccgcgcgtct tcggcgagcg
    14221 cgcaatgcgc gcgcttatgt cgtcgatcgc cgggttgctg ccgcggaaag gcgcgtgcta
    14281 aagtcatcct gcagatgctc attccgcatt gtgagacttt cgtgcactaa caggaggaac
    14341 ggcatgcaga tttcgcgtcg atggagcttc cgggttgcgg cagtcattgc cggtgcactt
    14401 ctggcggcga gcggtcgcgc cgccgaccct ccggcgccgg gcggtacggt gaccggcgac
    14461 acgatcatgc tcggacaaac cgccgcgcaa tcggggccgc aagcgccgat cggcgcgtcg
    14521 aagtgggggc tcgaggcgtt catcgcggcg gtcaatgcgc gcggcggcgt cgccggcaag
    14581 aagctgcagc tcacgagcta cgatgacggc tatcagcccg cgcagacgac gtcactcgac
    14641 aagaaactcg tttacgagga tcgcgtgttc gcgatcgtcg gcagcatcgg cagcccgacg
    14701 acagcggcgg tgtacaagaa gctgaacgaa gcggaggtgc cgctgatctc gatgggcagc
    14761 ggcagcccca tcttctacga gccggggctc aagtacgtgt ttccgtcctg gccgatctac
    14821 acgaccgacg gcaagacgat ggccgcgttc gtgaagaagc atttcgccgg gcagtcgacc
    14881 gccgtgatct accaggacga cggcttcggc aagccgatcc tggaggggat caagagcggc
    14941 cttggcaaga tcgacgtcga gatcccgtac gtcccgtcgc aggtcgactt cagcagcgcc
    15001 gtcatcaagc tcaaatcgtc ggggatcaag gtcgtgatgc tcgcgacgat cgccacgtcg
    15061 ggcgcgcaga tcctgaacca gatggcgagc ctcgactatc acccgacccg catcctgacg
    15121 tcgtcggcgt gcgggtacgg cggcatcttc agcgcgatcg catcgctcga aggcacgtat
    15181 tgcacggcgt tcctgccgcc tccgggaagt gcggatgcgc actggaagga attcgcggac
    15241 gcgatgggca agtacgcgcc agggcacccg ccggacatct acgcggcatg gggttggctc
    15301 gccggccagg tcacctacgc aggtcttgcg aaaatcaagg gcccgatcac gcgcgaggcg
    15361 ttcgtcgcgc aactcgagac gctgcgcgac ttcgacacga tcgccggaaa gatcaattac
    15421 tcgccgcaac ggcacgaagg cagctgctgc cagttgctgt ggcaggcgaa gggcggcaaa
    15481 tgggacatcg tccccgatag cgcattcaac ggctccgcca tgaaatgacg gcgcgtcgtc
    15541 atgacgctgt atctcctcca gatctgcctc gcggggctgg cacagggtag tcttttcgcg
    15601 ctcgcggcac tggggctcgt cgtcatcttc aacgcaagca ccgtcgtgaa tttcgcgcac
    15661 ggggcgatgg ggatggcggc gacctacgtg gcgtacgtgc tgatcacgtc gttccacgtc
    15721 ccgttcgcgc tcgcgttcgc aggcgcgatc gtcgctgcat tcgtgctcgg cgtcgtcgtg
    15781 cagaagctcc ttctcgggcg tctgcagggc gcgccggtgc tgacacagat cgtcgtgacg
    15841 ctcggtctct tcatgttcct cgaaggcatc ctcgggctcg cgttcggcta caacccgcgc
    15901 ccgatggcgg cgccggtcac catgcggccc gtcattctcg gcgggctcgt cctgcgtccg
    15961 caggacgtcg tgcagctcgg cgtcctcctc gtgctgtgcg tggcactcgc cgcgctcttc
    16021 ggcgcgacga agctcggcct cggcatgcgg gcgatcaccg agaaccccta tgcggcacgc
    16081 ctgatgggca tcccggtcgg aagcgtgctg gcaacggcgt ggggaatcgg cgtcctgctt
    16141 gcggcggtgg cagcgatgct cgcggcaccg accacctccg tcacgccgaa catgatggac
    16201 accatcgtcg tgtacgcctt cgtcgccgcg atactcggcg gcttcggctc gctgatcggc
    16261 gcattgatcg gcggtctcgt gctcggtgtg gtggtcaacc tgatcaccgc tttcctcgca
    16321 cccgaactcg cgatgtcgat cgttttcggg ctcctgctgc tggtgctgta cgtgaagccc
    16381 gacggattgt tcggtcgcgc cagcgtgcag aaagtctagg gccgcccgtg tcgcgaaaca
    16441 cgctcgtcat tcttctcgtc attgcatgcg ccttgttgcc gctcgtgctg caggtgatgg
    16501 gacaacgcca ggtttactgg ctgttcgtcg tctccgagct tttcatcctc gcgatcgtcg
    16561 cggtcagcct cgatctgctg atgggccgca gcgggcagat ttcgctcgga cacagcggct
    16621 tcgtggcact cggcgcgtac tcgagcgcca tcctgaacga gcgtttcggc gtcgacctgt
    16681 tcgtcggcgc gctcgccggc gggtcgctgg cggcactcgc aagcctcgta ctgggctttc
    16741 cggcaacgcg gctgcgcggc cattacctcg gcatcgtcac gctgggattc gggattgccg
    16801 tggcccagat cgcgctcaaa tggacctcgc tgaccggcgg cgaccagggc atccacctgc
    16861 acaagccggt gctgctcggc ctcgacgtcg cgacgcccgt gcgcatgtat tacgtcgcat
    16921 tggtcgcgct cgtcgtcgtc gtcctgctcg tctggaggct caccggcacc cgcgtcggcc
    16981 gcgcattcgc cgccatcaag gacagcgagg tcgccgccgc ggcgatgggc gtccccgtcg
    17041 cacgcacgaa ggtgctggcg ttcatctgca gcgcgttcct cgccggcacc gccggcggca
    17101 tctatgcgtt cctggcgggg ttcgtcgcgc ccgaggactt cggcatcgac agctcgcttc
    17161 tgtacttcgc gatggtgatc atcggcggca tgggatcgat tccgggcgcg atcggcggcg
    17221 ccgtcgtcgt ggacgtggtg aagaacgccg cggccacggt gagcggcctt tcgctcgcca
    17281 ttctcggcgg catgatcgtg ctcgtcgtgc tgttctttcc cggcgggctc aaggcgctgc
    17341 tgcgccgcca tccaatcgtc aagacgtcga cggccgagcg gcgcggcgtc gcggtggcgc
    17401 aagatgcttg aagcgcgcca ggtgacggtt cgcttcggcg gcctcgtcgc cgtcagcgag
    17461 ctcgacctcg tcgtgcccga cacgggcgtg ttcgcgctcg tcggaccgaa cggcgccggc
    17521 aagacgacgc tcatcaacgc actgagccgc gtgtgctcgg tcgccgaagg cacgatccgg
    17581 ctggacggcg tcgacgttct cggcctgctg ccgcaccagg ccgtgcgcgc cggcatcgca
    17641 cgcagcttcc agcgcgccga gcttttcccg acgcttaccg tgctcgagaa cctgctcgtc
    17701 ggcctgcacg cgcagatgac gaccggcctc gaaggcgcgc tgatgacgcc ggcgacccgc
    17761 gcgcaggaga agtcggcgcg ctcgcgtgcc gaacggctgc tgcgcgagct cgacttgtgg
    17821 gagctgcgca atgcgtctcc cgccgatctt ccgtacggcc accagaagct gctcgatgtc
    17881 gcgcgtgcgg tgatctcgga acccaggctg ttgctgctcg acgagccgtt cgccggactc
    17941 accgatgccg agtcgccgcg attgctcgca tgcatcgccg ctgccgggaa gcgctgcgcg
    18001 gtattgatga tcgagcacca cttcgagctg ctcgagggaa tcgccttgcg catgacggtg
    18061 atgaatttcg gccgcaagat cgcggagggc tcgcccgcac aggtccgcgc cgacccggag
    18121 gtcgtccgct gctatctcgg taccggccgc ccgaaggtcg cggaagccgc gacgtgctga
    18181 agctcgacga cgttcacgtt cactatggcc gcgtgcacgt gctgcgcggg atcagtctcg
    18241 aggttcccga cggcgcggtc gtggcgctgc tcggcgcgaa cggtgctggg aaaaccacga
    18301 cgctgaagac catctccggc atcctcaagc cctcgcgcgg gagcgttcat ttcgacggcg
    18361 aggatatctc gcgcctcgat gccgccgaca tcgtgcgccg gggcatcatt cattgccccg
    18421 agggacgcca ggtgttcccg cagctttcgg tgcgcgagaa cctgatgctc ggcagttatg
    18481 cgcgcggcaa tgcgcgcgtg cagttcgagc gcgtgctcga catctttccc gcgctgcgcc
    18541 cgcgcctcgc gcagcttgcc ggaacgctgt ccggcggcga gcagcagatg ctcgcgatcg
    18601 gaagggcatt gatgggcgag ccgaaacttt tgctgctcga cgagccgagc ctcgggctcg
    18661 ccccgctcat cgtcgagtac atcttccagg tgatcgccaa attccgcgag cacgacatct
    18721 cggtgcttct gatcgagcag aacgcgatgc tcgcactcga gttcgccgga tgggcctatg
    18781 cgctgtcgca tgggcagata acgttctcgc acgcgaccca ggcgctgcgc gacaccgagc
    18841 tcgtgcgccg tgcgtacctc gcatgatcgc gttatcatat agaacgctat ttcacatagt
    18901 gagaatttcg gaggtgcatc gatgattcgc aacagctggt acgtcgccgg tctttcccgc
    18961 aacttcaagt acaagctcga gaaaaagatc atcaccgcga tgccgatcgt gatgtggcgc
    19021 gcgcaggatg gaaacgtcgt cgcgttcgac ggccgctgct gtcacaagcg cttcccgctg
    19081 tgggacggaa agctgctcga caacggcgtg ctgcgctgcg cgtaccacgg gctctgctac
    19141 gacagctcgg ggaaatgcat cgacatcccg atgcagcgcg acatacccat ctcgccggtc
    19201 gcgcgccttc gccgctatcc ggtcgtcgag caggacgggc tcgtgtggct ctggcccggc
    19261 gacccggaga agatcggaaa cgtcaaggcg ccgcgcatac cggagcttgc cgatccgcgg
    19321 tacgaggcgg tgatctccga tccgccgatc cgcattcgcg ccaattaccg gctgctgatc
    19381 gagaacctgc tcgacatcac gcatttcttt ccgctgcacg acggcaacat cggcgacctc
    19441 gccaactcga tgatcccggt gggcctcgtc gaagacaccg tcgacggaaa cccgaaggtg
    19501 atgaccacgc gcagcgttac gaattaccgt ctccctccgt actaccagcg ctggttcggc
    19561 ctcgacatcg tcgatcgcga tcacacgcac gcgatgaccg gccccgggct cgtgcgggtg
    19621 cagcttcggc tcgccccgcc ggggaagctc ggaaccgacg acgagtgcgg ctacatcctg
    19681 tgccacaccg atacgccgat cgacgagcag catctcgagt ggcactggat catgatctcg
    19741 aaggcagggt cgcgtttccc acccgacccg tcgatgacgc tggcgcaagg catggcgttc
    19801 gagtttccgg aggtggtcgc gcaggacgag tgggcgctcg ccaagcagca ggagatgctc
    19861 gaattctccg acgcgttccc cgacgccacg cgttaccgcg agatcaacat gcgctccgac
    19921 atcggcgtcg ttcacgcgcg gcgtgtgctt tcgcgcatgg agaaggccga gaacaacgag
    19981 ccgttcgggc tcccgtccgc gtcgctcgag atggcgaagt acgcggccgc gcccggggca
    20041 cagggagcgg ctgcgcccga gacacacgga gccgtccaat gaaggtggtc gtcgatttcg
    20101 acctgtgcat gggcaacgcg cagtgcatgc tcgccgcacc gcaggtcttc gaagtgcgcg
    20161 acgacggcat gctctacttg ctgcaggaag agccgcccga agctctgcgc gccgccgtcg
    20221 agaacgcagc gcgcctgtgt cccacgcagg cgatcaccat cgaaaaatag cgcgtgcgct
    20281 cggtcgtcat cgtcggtgcg tcgctcgccg gcctttccgc cgcgaaggca ctgcgccggc
    20341 aaggcttcga cggcacggtc acgctgatcg gtgacgagcc gcatcgtccg taccagcggc
    20401 cgccgctgtc gaaacagttg cttggcggcg cctgggaccg cgcgcggctc gaccttcccg
    20461 accgcggtga cctctgcctc gactggattc tcggtacggc tgccgcctcg ctcgaccttg
    20521 caactcgcga agtgacgctt gacgacggcc ggcgcgtgcg cttcgacggc ctcgtgatcg
    20581 ccaccggcgc acgtgcgcgc aagccgcgct ggcatgccga gctgcgtggc gtcttcacgt
    20641 tgcgcacggt cgaggacgcg ctcgcgctgc gggcacacgt tgcggcgggg catcgcaatg
    20701 tggcgatcgt cggtgcggga ttcatcggct gcgaaacggc ggccacgctg cgcagcagcg
    20761 gctgcgacgt gacactgatc gactgcgagc gcctgccgat gctgcgcgca ctcggcgaat
    20821 cgcttggcgc catgtgtcgc gagctgcacg aggcgcacgg cgtgcggacg gtattcggcg
    20881 ccggcgtcga ggcgctgctc ggtcgtgaat cggtcgaagg cgtgcggctc tccgacgcca
    20941 gcatcgtcga cgccgactgc gtcgtcgtcg gtgcaggcgt cacgcccaac gtcgaatggc
    21001 ttgccggctc gggactcgtc gtcgacgatg gtgtggtctg cgactcgacg tgcgcagccc
    21061 tcggcagcga ggccgtcgtt gccgcgggcg acgtcgcgcg ctgggagcac ccgcattacg
    21121 gcctgcgccg catcgagcac tggaacaatg ccatcgcgca ggccgatgcg gccgcgggca
    21181 cgctgctcgc gatgccgcgc aggggggtgc cctacgccgc cgttccgttc ttctggtcgg
    21241 atcagtacga ctgcaagttg cagttgatcg gcgcgccggc ggcgggcgat gcgacacgcg
    21301 tggtcgaggg cgcactcggc gagcgccgct tcgtcatgct gttcgagcgc gaaggccgcg
    21361 tgagcggcgc attccttttc aacagcatgc accgcgtggc ggcttacacg acggccgtcg
    21421 aaggcgccgt tgggtgcgtc gccgaaggcg ttggatcatg agcgccgccg ggccgcccca
    21481 aggcgcgaac cgccccgcag cgcgaagtgc tgcgggtagt ccagtgaccg ccgccgaaac
    21541 gcctcctcca ttgctcgcgg ggctgcgggt gctcgaattc ggccacattg cagcggcgcc
    21601 gttctgcggc atgctgctcg cggacctcgg cgccgacgtc gtgaaggtcg aagcgccgca
    21661 cggcgacggc ttgcgtgcat ggcccccgat cgtcgagaca cccgacggcg actcgttcag
    21721 cctcaacttc gcgtcgctca atcgcggcaa gcgcagcgtc gtcgccgatc tcaaggaagc
    21781 ggccgagctc gcgcgcattc gcgatctgtg cgcgcatgcg gatgtgatca tcgagaacta
    21841 ccgcccgggc gcgctcgacc gcctcgggat cggcttcgaa gatatcgcca cgctccagcc
    21901 gacgatcgtc tactgctcga tctccgggta cggacatcag ggcgcgaacg cgacccgggg
    21961 tgcgttcgac gtggtggttc agggcgcatc gggactgatg agcgtgaccg gcgagcccgg
    22021 gcgaccggcg gtgaaatgcg gcgtgccggt cgccgacttc acggccggcc tgtacgcggc
    22081 gtacacgatc gtcgccgcgc gcggccaggc gttgcgggag cggcgcgcaa tacacctcga
    22141 ctgtgcgatg ctcgattgcc tgctcggcat atcggcgctg cagacgagcg agtactgggg
    22201 caccggcact gcgccgaaac ggctcggctc ggcccacccg cgaaatgcgc cgtaccaggc
    22261 gttcgacgcg agcgacggcc agttcatcct cgccgccgga aacgacgcgc tgtggcgcga
    22321 agtatgcgaa gcgaccgggc agcacgcgct gctcgacgat cctcgctttc gcggcatttc
    22381 gctccgcgcg aagaaccagg cggcgctcgc cgccctcctt cagcaggtgt ttcgcacgcg
    22441 aacggtccgc gaatggctcg acgagttcga gcggcgcggc gttccgagcg gtccgctcaa
    22501 cacgttcgcg gacatcctcg gcgatccgga actcaccgcc agcgagctcg ttcaggacat
    22561 gcgcgtgccg gttgccggca ccacgaagac cgtggcgtac ccggtgcggc tcaacggcca
    22621 tcgcatgcgt gcgcagcgcc cgccaccgcg cctcggcgag catcgcgacc aggtgttcgc
    22681 cgagtggttg ggcgcgagcg cgtcgagcga gcgcgcatga gaggagcgtt gcgcgtcgag
    22741 caggatgcgc cgcgggccgg cgtatggcgt ctcgtgctcg acgacgcgcc gcatgcgaac
    22801 gcgctgtccc cggcgctcgt cggccgcctg caggcggccc tgcacgccgc gttcgagcgt
    22861 gatgtccgct gcatcgtcat cgccagcagc agcgagcgat tctgcgccgg cgccgatctc
    22921 gccgatgccc accacctcga cgactcggcg ctgcgcgacc gcttcagcgc gctcgaggat
    22981 ctgctcgaga ccgtgcggcg tgctccggca ctgacgatcg cggtggtgcg cggcgcggcc
    23041 ctcggtgcgg gcgccgatct cgtgacatcc tgtgattacc gccttggcac acccaaggcc
    23101 cgcctcgcat ttccggggag ccggttcggc gtggtcctcg gaacgcggca cctcgcggcg
    23161 gtgattggaa ggcaagcggc gcgcgagctt ctcgtcgagg gaaagtcgct cgatgcacaa
    23221 gcggccgcgc aatgcggact gctgagcgag ctttgcgccg aggacgccat cgattcacgt
    23281 gtggaaagca tcgcgcaaag ctcggaagcg atcgacgctc cgacgctgcg tgcgatcctg
    23341 cgtcttactc gggagcttcc ttccgagcgc gatcgcgcgg agctcctgca atcgacatcg
    23401 cgcaacggcc tcgccgaacg gttgcgcgag cacgcgcgca gcgcacagga gcagcgcgca
    23461 ttgcgacgcg cggccgggca atgaaatcgg cggccgcaac gcgccgttcg gcgccaccca
    23521 agcccgtgcc tgcacgcttg agcgcgcagg gccgccccaa gcgcgaacac ccgagtgcga
    23581 agcacgaagg tggtcgattg agctcggtgg cgaaggcgct tctcgtcctc gaatgcattg
    23641 gcctcgcgaa gcggcgaacg ctgaccgaga tcgcgcacga agtcacgctc ccgaaatcca
    23701 cgctgctgcg tctcatccag acgctgatcg cacgtggctt cgtgcgccgc acggcacacg
    23761 gcgaatacgc ggtcagcctc aagctatggc agatcggatg caacgccctc gactccgaag
    23821 cgatacgcga cgaggtcatc ccgttgctga acgatctcgt cggcgcgacc ggcgagacgg
    23881 cgcattacgc ggcatacgaa ggtggattca gcgtctatat cgagaaggtc gacggcctgc
    23941 atccgatccg ctcgtatacg gcggtgggcg gacgctcgcc cgcgtatgcg accgcaacag
    24001 ggaaggcgct cctcgcgtgg cgggacgagc gcgaaatcgc caaggtgggt cgctcggcgc
    24061 gacgctggac cgacgcgacg caagccggtg ccgacgaggt gttgcgcgag gcggagcaaa
    24121 cgcgccgggt cggctatgcg gtcaaccgcg gagagtggcg cgcatccgtg tggggaatcg
    24181 gcgcgccggt gttcgaccgt catgcgcagg tgatcgccgc ggtgggcatt tcgggcccgc
    24241 gcgagcgcat cgagcccaac gtgcgaccct acgcaaaact ggtcgtggcc gcggcgaagg
    24301 cgctgtcgcg tcggatggga tcgcccggct gatccgcgcg ccttgcacgc tcatcgccag
    24361 gtcgtgtaac gcttacggac gccggcgcaa ttattgccac cccgcatgcg ccggtgaccc
    24421 cggcgacggt gtgccggcag cgtgcgaatc ccatgccctc atttgcggct gcgcctgacg
    24481 gcgccattga ttccgggccg cttctcgatc gcctcgatcg cttccacttc ggcttccggg
    24541 ttgtcgtcgg actcgacgct gtcggcctcg cgctcggtga gcgagtcgtc catgacgttc
    24601 atcgacccgg tctcgtcggc gtcgtgctcg gtgagcactt cgtcgacggc ctcggttgaa
    24661 tcgcggccgg cggccgcgcg ctcgcccgtc ccatagcggt cgctgtcgga atcgaggtcg
    24721 gcgtcgccga gatcggggcc tgcagtcgtc ggcgccgaat cgacatcggg atccgacgtg
    24781 gtcccccgct gcagcggcaa tccgccgtcg cgattgagtc ccggaccgcc cacgatgtcg
    24841 ctgccgctgt ccgtggagtc gctcggtccg agcgcgcccg ttccatgccc cttttgcgtg
    24901 ccgcccttgc cttcgccggt ggtgttcagt gtgctgcgtg ccatgattcg tcctccgttg
    24961 cgaaatgaat tcgacgcaag gcacgtgcca ttgcccgctt cgacaaatcg ggccgattcg
    25021 ggcacgattg caggcgtgcc gtcgtcgcgg cgcgcttgac gccgcgttca cgtgaatcgg
    25081 gagtgatccg catgctacgg gcgatgccgg ctttcctgct cgcattcatc gcatcgctcg
    25141 catcgctggc gcacgcggcc gacatcgggc agatcaagat tgcgaagggc acggtgacga
    25201 tcg
//
LOCUS       DASSVX010000018        10084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_750420, whole genome shotgun sequence.
ACCESSION   DASSVX010000018 DASSVX010000000
VERSION     DASSVX010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750420"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1230
                     /locus_tag="VFO53_00905"
     CDS             256..1230
                     /locus_tag="VFO53_00905"
                     /inference="COORDINATES: protein motif:HMM:NF016552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874859.1"
                     /translation="MRSAAVVAVSCLAVMVSSAVNAGAVRADVDELVPTGKGWGERPA
                     PGPGPGEGAGQGQGQPQGQGKPSRPTSGNGIDYHGGPVMVDSAKGPPNIYYIWYGNWS
                     GNSATTLLTNLAQNIGGSSYLRINTTYYNGAGIHVSGKTNFPSLQNSAQDAYSQGTSF
                     GDAGVQAIVAKAINSQALPLDSNGVYFVLTSADVNETSGFCTQYCGWHTHGTITGTDI
                     KFAFVGNPDRCPSACAEQTTSPNGNTGADGMASIVSHELEEAISDPDLNAWYDRRGQE
                     NADKCAWTFGSTTTLKTGAKYNVTLGGTNYLIQQNWVNASGGYCAMSF"
     gene            1323..1832
                     /locus_tag="VFO53_00910"
     CDS             1323..1832
                     /locus_tag="VFO53_00910"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET8874860.1"
                     /translation="MRLRLVLLAFAACVVAPALAQPAPAPGRIPTVTRLVKLFTGLEM
                     DLVARTHAKDLSALDALLDPSFELRAGNTPGTPVPRDAWIRDARAAPRAPPRIDQMSV
                     HDFGDVAIVSFRETAVAGATRGGSARFVVDCWKRDGDAWRLAVRYVSDASAQGVKLPS
                     AAATIDKRY"
     gene            complement(1826..2536)
                     /locus_tag="VFO53_00915"
     CDS             complement(1826..2536)
                     /locus_tag="VFO53_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8874861.1"
                     /translation="MADLLALDGVTAGYGASVVLEDVSLSIGEGDSLALLGRNGVGKT
                     TLLVTLMGLTRVHRGTVRFRGTAWTRLPTFRRAQAGIGWVPQERYMWPSLTIEEHLTA
                     VARPGRWTLAKVYEIFPRLAERRGNFGTQLSGGEQQMLAIARALVVNPALLLLDEPME
                     GLAPIIVQDLMRVIAGLVREGGMAVVIVEQHAKLALSVTQNAIALDRGRIVHRSSSES
                     LLDDPQTLHRLVTLGSDQ"
     gene            complement(2529..3272)
                     /locus_tag="VFO53_00920"
     CDS             complement(2529..3272)
                     /locus_tag="VFO53_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8874862.1"
                     /translation="MLRTEGLSKRFGAFAAVSDVSLSFAPGVRHALIGPNGAGKTTFI
                     NLLTGFLAPTAGNVFLGERLITGLPQHERVKCGMTRTFQINTLFPGLTVLESVVLAVC
                     ERQRRAGVWYSTVSRQAEAIDEAIALLESLRLVNERNELTRNLAYGKQRLLEIALALA
                     TRPSILLLDEPAAGIPSAESAELFGVIARLPRDVTIVFIEHDMELVFRFAERITVLVG
                     GSVLTEAAPSEIARDPRVKEVYLGEAQHG"
     gene            complement(3317..4285)
                     /locus_tag="VFO53_00925"
     CDS             complement(3317..4285)
                     /locus_tag="VFO53_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8874863.1"
                     /translation="MRAIALPNDRFTLLEIAFWLLPVIAFFAFPNYRVLGSQVLITGL
                     FAVSLDLILGYAGIVSLGHAAFFGVGAYTAGLLAVHGWHEPISGLFVAAIAAAIAGYL
                     ASFLVVRGSDLTRLMVTLGIGLMLWEAANKAAFVTGGVDGLSGVTIAPILGLFRFDIG
                     GRTAFVYALVVVFIVFVIVRRIVHSPFGLSVRGIRESGKRMPAIGAPVAKRLRTIFTI
                     GAAVAGIAGALIAQTTQFVGIDTLGFSRSAELLIMLVLGGAGRLYGGLVGAAVFIVAQ
                     DYLSGIDPVYWEFWIGFLLVAVVLFAHGGILGGLDALRARWARRTQ"
     gene            complement(4323..5189)
                     /locus_tag="VFO53_00930"
     CDS             complement(4323..5189)
                     /locus_tag="VFO53_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8874864.1"
                     /translation="MIGNLIGVLFDGLAYGSLLFIIAVGLSVTLGLMNFVNLAHGAFA
                     MVGGYVCVMLMTRAGVPFLATLPVAFVAAAIVGLVLERVLYQRLYRSSPLDQVLFSIG
                     IVFMSIAIATYYFGASQQPVSLPPYLRGQVHVLGLDLGVYRLFLIVVVALLTLALQYL
                     LDRTRFGSQVRASVDNATAAAGLGINVDRVFALTFALGSGLAGLGGGLGIDVLGLDPT
                     FPLKYMVYFLLVVSVGGSGTIKGPLIAALILGVFDVAGKYYLPQVGGFVIYALMVVLL
                     IVFPAGLYGRRA"
     gene            complement(5224..6405)
                     /locus_tag="VFO53_00935"
     CDS             complement(5224..6405)
                     /locus_tag="VFO53_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8874865.1"
                     /translation="MKQARLLVSALGALAALVAMPAAHAQNTIKVGVIAAFSGPFADY
                     GGQIEAGMKAYMKEHGDSVAGKKVELITRDTKGPAPEVAKRFAQELITRDHVQFLAGF
                     GLTPNAMAVAPVVSEAKVPMVISNAATSSITTKSPYITRVSMTIPQIAEPLAQWALKN
                     GIKQVYTVVADYGPGIDAETQFTKTFKAGGGEIVGSVRTPLQSPDFSAATQRVKDVKP
                     QAVFVFLPAGQQGISFMKSFNERGLGEAGIKLIATGDITDDHVLEAMGDPTLGMITAF
                     HYSAAHKSPENEAFLKAYAAANDAKAGAPNFMAAAGYDTMHVIYEVAKKLDGNIDGDK
                     AMAAIKGMKWMSPRGPVTIDPETRDIDQTVYIRKVEKVNGKLANVEFDKIPDVKDPGK
                     K"
     gene            complement(6486..7751)
                     /locus_tag="VFO53_00940"
     CDS             complement(6486..7751)
                     /locus_tag="VFO53_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET8874866.1"
                     /translation="MANLDERTLLRRMFDAAVESAQPAHALPPCLPDPPRGRTLVVGA
                     GKASAAMARALEEHWRGALRGIVVTRYGHAVRCEHIEVVEAAHPVPDAAGVAAARRVL
                     ASIAGLSRDDLVVALISGGGSALLTLPAPGISLADKQQVNRALLASGAAIGEINCVRR
                     HLSAIKGGRLAAACHPARVVTLAISDVPGDNPIDIASGPTVGDPTTRADARAVLRRYG
                     VDVPPTVRALLDTETAESVKPGDARLARADYRIIATPQKALDAAAQVARAEGVTPVIL
                     SDSIEGEARDVGKVLAALARQAALRKEPFVPPCVLLSGGETTVTVRGDGRGGRNVEFL
                     LALALALQGRRGIYALAGDTDGVDGVEEIAGAIATPDTLVRGLEQGRHAADDLDRNDA
                     HAFFEALGDGVITGPTRTNVNDFRAILIM"
     gene            complement(7775..8734)
                     /locus_tag="VFO53_00945"
     CDS             complement(7775..8734)
                     /locus_tag="VFO53_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET8874867.1"
                     /translation="MEIFARVVEARSFSGAARRLNVSTSVVSKCVTRMERSLGVRLLN
                     RTTRSISLTEIGREFYARCAHIVAAAEDAEAMTAGMQSTPRGTLKVNVPVSFGVLHIV
                     PVIAGLLATYPELHVDMTLNDREVQLAEEGYDVAILIAAEPRATLVARKLAPIRQALC
                     AAPAYLRRHGAPASVADLAHHDCIVFSRLGPERHWHFDSADGRASVEVSGALRLDNEN
                     AVRQAALAGCGIALLPTYIVGGDLQQGTLRTVLPEYKAAETALYATYLPNRYLAAKVR
                     VFVDYLVGRFGPSPGWDALRKMPAAGGRRAGDERPGAGSADPR"
     gene            8948..9655
                     /locus_tag="VFO53_00950"
     CDS             8948..9655
                     /locus_tag="VFO53_00950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HET8874868.1"
                     /translation="MRFIPARRRILKAALIPSAASVVPLRFVRDAAAEPVSAPGQFAY
                     FTPVEFTWVDAAVARLIPNDELGPGAKEAGVATFIDRQLSGPYGRAETWYMQGPWKEG
                     TKEQGYQLKMTPAQLYRTAIADVDAWCSSSGKRPFAELEGAAQDKLLHDLEKGNIKLT
                     RAPAKDFFQMLLQNTVEGFLADPMYGGNRDFAGWKLIGFPGPRYNYIAEIDQYGKRYA
                     MPTVGLLGRDGTRVKET"
     gene            9655..>10084
                     /locus_tag="VFO53_00955"
     CDS             9655..>10084
                     /locus_tag="VFO53_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET8874869.1"
                     /translation="MATKLPSVDVVLVGFGWTASIIGQELTDAGLQVVALERGAFRDT
                     VPDFATTQIQDELRYAVRNELFEEPERETLTFRNSMDQTALPMRHLGSFKPAAGAGGA
                     GVHWNGQNWRFLPTDFLQRSHIEQRYGKGFIPADMTIQDWG"
BASE COUNT         1774 a         3404 c         3341 g         1565 t
ORIGIN      
        1 ggcgcaacgc aatcacctct acacgctcca attcctgctg actcattccg gtctccatcg
       61 cgccccctgc ttgctggagg ctggaacgta ccggacattt ctactttgct aagaccggac
      121 attagcgcgt tgccgcgaca cgctgcgtgt tcgcccttgc gcaacgctta tgcctggagg
      181 atattccgtc cgcggccggt ggaccgtcag ttcatgtaat ggccgcgtga actcattcga
      241 tcagggagag cagtcatgcg tagtgcagca gtcgttgcag tgtcgtgcct ggccgtcatg
      301 gtgagcagcg cagtgaacgc gggcgcggtt cgcgccgacg tcgacgaact cgtccccacc
      361 ggcaagggct ggggcgagcg gccggcaccc ggacccggtc ccggagaggg cgcagggcaa
      421 ggccaggggc agccgcaggg acagggcaag ccgtcgcggc cgaccagcgg caatggcatc
      481 gattaccacg gcgggcccgt gatggtcgat tccgcgaagg gcccaccgaa catctattac
      541 atctggtacg gcaactggag cggcaacagt gccacgacac tgctcaccaa tctcgcgcag
      601 aacatcggcg gctcttcgta tctcaggatc aacaccacct attacaacgg agcgggcatc
      661 cacgtgagcg gcaagacgaa tttcccgagt ctgcagaatt ccgcgcaaga tgcctattcg
      721 caagggacgt cgttcggcga cgccggcgtt caggccatcg ttgcgaaagc aatcaacagc
      781 caggcattgc ctttggattc caacggcgtc tatttcgtgc tgacatcggc tgacgtcaac
      841 gaaacgtccg gcttctgcac gcagtactgc ggctggcata cacacggaac catcacgggg
      901 accgacatca agttcgcgtt cgtcggcaac cctgaccgat gccccagtgc atgcgccgaa
      961 cagacaacca gcccgaacgg caacacaggc gccgacggca tggcgagcat cgtctcgcac
     1021 gagctcgaag aggcgatcag cgacccggac ctcaacgcct ggtacgaccg tcgcggacag
     1081 gagaacgccg acaagtgcgc gtggacgttc ggtagcacga cgacactgaa gaccggggcg
     1141 aagtacaacg tcacgctcgg cggcaccaac tacctcatcc agcagaactg ggtcaacgcg
     1201 tccggcggct attgcgcgat gagtttctaa tcgctttcga gaaacgagcc ggtcatgaac
     1261 gttcgggcgg cgccagcgag cgccgcccga acgtttcccg gtgcgggtcg ctcgaagtct
     1321 gcatgagatt gcgcctcgtc ctcctggcat tcgccgcgtg cgtcgttgca ccggcgctcg
     1381 cgcagcccgc gcctgcgccg ggccggattc ccaccgtcac gcggctggtc aagctgttta
     1441 ccggactcga gatggatctc gtggcgagaa cgcacgccaa ggacctgtcg gcgctcgacg
     1501 cgctcctcga cccttcgttc gaattgcgcg ccggcaacac gcccggcacg ccggtgccgc
     1561 gcgatgcctg gattcgcgat gcgcgcgctg cgccacgagc gcccccacgc atcgaccaga
     1621 tgtcggttca tgacttcggc gacgtggcga tcgtcagctt tcgcgagacc gccgttgctg
     1681 gcgcgacgcg cggcggatcc gcgcggttcg tcgtcgactg ctggaagcgc gacggcgatg
     1741 catggaggct cgcggtgcgc tatgtgagcg acgcatcggc tcagggcgtg aagctgccga
     1801 gcgccgccgc gacgatcgac aaacgctact gatccgatcc gagcgtcacc aggcggtgca
     1861 gcgtctgcgg gtcatccagc agcgactcgc tcgatgaccg gtgcacgatg cggccgcggt
     1921 cgagcgcaat cgcattttgc gtcaccgaca gcgcgagctt cgcgtgctgc tcgacgatta
     1981 cgacggccat gccgccttcg cggaccaggc ccgcgatcac cctcatcagg tcctgcacga
     2041 tgatcggcgc gagaccttcc atcggctcgt cgagcagcag cagcgcggga ttgaccacca
     2101 gcgcgcgcgc aatggccagc atctgctgct cgccgccgga aagttgcgtt ccgaagttgc
     2161 cgcggcgctc ggcgagtcgc ggaaagatct cgtacacctt ggccagcgtc cagcggccgg
     2221 gccgtgcgac cgcggtcagg tgctcctcga tcgtcagcga gggccacatg tagcgctcct
     2281 gcggcaccca gccgatgccg gcctgcgcgc ggcggaacgt gggcagccgc gtccacgccg
     2341 tgccgcgaaa gcgcaccgtc ccgcgatgca cgcgggtgag ccccatcagc gtgacgagga
     2401 gcgtcgtctt gccgacgcca ttgcgtccaa gcagcgcaag gctgtcgcct tcgccgatcg
     2461 acagcgacac gtcctcgagc acgaccgacg cgccgtaacc ggcggtcacg ccatcgagcg
     2521 cgagcagatc agccatgctg cgcctcgccg agatacacct ccttgacgcg cggatcgcgg
     2581 gcgatctcgg acggcgcggc ctccgtcagc acgctgccgc cgacgagcac cgtgatccgc
     2641 tcggcgaagc ggaagacgag ctccatgtcg tgctcgatga acacgatcgt cacgtcgcgc
     2701 ggcagacgcg cgatcacgcc gaacagctcg gcgctttcgg ccgacggtat gccggccgcc
     2761 ggctcgtcga gcaacaggat cgacggccgg gtggcgagcg cgagcgcgat ctcgagcagc
     2821 cgctgcttgc cgtaagcgag attgcgcgtg agctcgttgc gttcgttcac gagacgcagc
     2881 gattcgagca gcgcgatcgc ctcgtcgatg gcttcggcct gccgcgacac cgtgctgtac
     2941 cagacgccgg cgcggcgctg ccgctcgcac acggcgagca cgacggactc cagcacggtc
     3001 aagccgggaa acagcgtgtt gatctggaac gtgcgtgtca tgccgcactt gacgcgctcg
     3061 tgctgcggca acccggtgat gagacgctcg ccgagaaaca cgtttcctgc ggtcggcgca
     3121 aggaagccgg tgagcagatt gatgaacgtc gtcttcccgg cgccgttggg cccgatcagc
     3181 gcatgccgca cgccgggcgc gaacgacagc gagacatcgc ttaccgcggc gaacgcgcca
     3241 aagcgcttcg acaggccttc ggtgcgaagc atggaggatg gattgccgcg cgcctgaggg
     3301 ttccccgcca gtgcgttcat tgcgtacgcc tcgcccatcg agcgcgcagc gcgtccagtc
     3361 cgccgagaat gccgccgtgc gcgaacagca cgacggcgac gagcaggaag ccgatccaga
     3421 attcccagta gaccggatcg atgcccgaca ggtagtcctg cgcgacgatg aatacggcgg
     3481 cgccgacgag cccgccgtac agccgccccg cgccgcccag cacgagcatg atcagcaatt
     3541 cggccgaacg cgaaaagccg agcgtgtcga tgccgacgaa ttgcgtcgtc tgcgcgatca
     3601 gcgcgcccgc gattcccgcg acagcggcgc cgatcgtgaa gatcgtgcgc aggcgcttcg
     3661 cgaccggcgc accgatggcc ggcatgcgct tgccgctttc gcggatgccg cgcacgctca
     3721 agccgaacgg cgaatgaacg atgcggcgca cgatcacgaa cacgatgaaa acgacgacga
     3781 gcgcatagac gaacgccgtg cgcccgccga tatcgaaccg aaacaggccg aggatcggcg
     3841 cgatggtgac gccgctcagg ccgtcgaccc cgcccgtcac gaaggcggcc ttgttcgccg
     3901 cttcccatag catcaggccg atgccgagcg tgaccatgag acgcgtgagg tcgctgccgc
     3961 gaacgacgag aaagctcgcg agatagccgg cgatcgccgc cgcgatcgcg gcgacgaaga
     4021 ggcccgaaat cggctcgtgc cagccgtgca ccgccaggag tccggccgtg taggcgccga
     4081 cgccgaagaa ggcggcgtga ccgagcgaga cgatgccggc gtatccgagg atcaggtcga
     4141 gcgagacggc gaaaaggccg gtgatgagca cctggctgcc gaggacgcga tagttcggaa
     4201 aggcgaagaa cgcaatgacc ggaagcagcc agaacgcgat ctcgagcagc gtgaagcgat
     4261 cgttcggcaa cgcgatcgcg cgcatccgcc gttgcgcgga cggcgcggcc accgcgctcg
     4321 cctcaggcgc gccggccata gagccccgcg gggaagacga tcagcagcac gaccatcagc
     4381 gcgtagatca cgaagccgcc gacctgcggc aggtagtact tgcccgcgac gtcgaacacg
     4441 ccgaggatca gtgcggcgat gagcgggccc ttgatcgtcc ccgagccgcc gacgctcacg
     4501 accagcagga agtagaccat gtacttgagc gggaatgtcg gatcgagtcc gagcacgtcg
     4561 atgccgagtc cgccgccgag gccggcgagg cccgagccaa gcgcgaacgt cagcgcaaac
     4621 acgcgatcga cgttgatgcc gagtcccgcg gcggcggtcg cgttgtcgac gctggcgcgc
     4681 acctggctgc cgaagcgcgt gcggtcgagc agatactgca gcgcgagcgt gagcagtgcg
     4741 accacgacga tcaggaagag ccggtacacg ccgagatcga gccccagaac gtgcacctgg
     4801 ccgcgcaggt acggcggcaa cgacaccggc tgctgcgagg cgccgaagta gtacgtggcg
     4861 atcgcgatcg acatgaacac gatgccgatc gagaacagca cctggtcgag tgggctcgac
     4921 ctgtagaggc gctggtagag cacgcgctcg agcacgaggc ccacgatcgc tgcggcaacg
     4981 aacgcgaccg gcagcgtggc gaggaacggc acgcccgccc gcgtcatcag catcacgcaa
     5041 acgtagccgc ccaccatcgc gaaggcgccg tgcgcgaggt tgacgaagtt catcaggccg
     5101 agcgtcaccg acaacccgac ggcgatgatg aacaacaggc tgccgtacgc gagaccgtcg
     5161 aacaggacgc cgatcaggtt gccgatcacg cggacctcgg ccggccgcta tcgcgcggcc
     5221 gcgctacttc ttgccaggat ccttgacgtc gggaatcttg tcgaactcga cgttggcgag
     5281 cttgccgttc accttctcga ccttgcggat gtaaacggtc tggtcgatgt cgcgcgtctc
     5341 ggggtcgatg gtcaccgggc cgcgcgggct catccatttc atgcccttga tcgccgccat
     5401 cgccttgtcg ccgtcgatgt tgccgtcgag ctttttcgcg acttcgtaga tcacatgcat
     5461 cgtgtcgtag ccggccgccg ccatgaagtt cggtgcgccg gccttggcat cgttggccgc
     5521 agcgtacgcc ttaaggaacg cctcgttctc cggcgacttg tgcgcggcgg aatagtggaa
     5581 tgccgtgatc atgccgagcg tcgggtcgcc catcgcctcg agcacgtgat cgtcggtgat
     5641 gtcgccggtg gcgatgagct tgatccctgc ttcgccgagt ccccgctcgt tgaagctctt
     5701 catgaacgag atgccctgct gccccgccgg caggaacacg aacaccgcct gcggcttcac
     5761 gtccttgacg cgctgcgttg cggcggagaa gtcgggactt tgcagcggcg tgcgcaccga
     5821 gccgacgatc tcgccgccgc ccgccttgaa cgtcttggtg aactgcgtct cggcgtcgat
     5881 gccgggaccg taatcggcca ccaccgtata gacctgcttg atgccgttct tgagcgccca
     5941 ctgcgccagc ggctcggcga tctgcgggat cgtcatcgac acgcgcgtga tgtacggcga
     6001 cttcgtcgtg atcgacgacg tcgcggcgtt cgagatgacc atcggcacct tcgcctcgct
     6061 gacgaccggc gcgaccgcca tcgcgttcgg cgtcagcccg aagccggcga ggaactgcac
     6121 gtgatcgcgg gtgatgagct cctgtgcgaa gcgcttcgcg acttcgggcg ccggaccctt
     6181 cgtgtcgcgc gtgatgagct cgaccttctt gccggcgacg ctgtcgccgt gctccttcat
     6241 gtacgccttc atgccggcct cgatctgccc gccgtaatcg gcgaacggtc ccgagaaggc
     6301 ggcgatgacg ccgaccttga tcgtgttctg cgcgtgcgcg gccggcatcg caaccagtgc
     6361 cgcaagtgcg ccgagtgcgc tgacgagcaa gcgtgcttgt ttcatcgacg ttcctcctga
     6421 cggccgtggg tccgcgtcat ttgcgcacgt ttcggcacgc gacgccggtt cttcgctcga
     6481 ggatactaca tgatcaggat cgcacgaaaa tcgttcacgt tcgttcgtgt cggcccggtg
     6541 atgacgccat cgccgagcgc ctcgaagaac gcgtgcgcat cgttgcgatc gagatcatcg
     6601 gccgcatggc gaccctgctc gagcccgcgc acgagcgtat cgggcgtggc aatggcgccg
     6661 gcgatctcct ccacgccgtc gacgccatcg gtgtcgcccg ccagcgcgta aatgccgcgg
     6721 cgtccctgca gtgcgagtgc cagcgcgagc aggaactcga cgttgcgtcc gccgcggccg
     6781 tcgccgcgca cggtcaccgt cgtttccccg cccgaaagca gaacgcacgg cggtacgaac
     6841 ggctctttgc gcagcgcggc ctgacgcgcg agtgccgcga gcaccttgcc gacgtcgcgc
     6901 gcctcgcctt cgatgctgtc ggagagaatg accggcgtca cgccttcggc tcgcgccacc
     6961 tgcgccgccg cgtcgagcgc cttctgcggc gtggcaatga tccggtagtc ggcgcgcgca
     7021 aggcgcgcgt cgcccggctt gacgctttcg gccgtttcgg tgtcgagaag cgcacgcacg
     7081 gtcgggggca cgtcgacgcc gtagcggcgc aacacggcgc gcgcgtcggc gcgcgtcgtg
     7141 ggatcgccca cggtgggccc ggaagcgatg tcgatggggt tgtcgcccgg cacgtcggaa
     7201 atggcgagcg tgaccacgcg cgccgggtgg cacgccgcgg cgaggcgccc gcccttgatc
     7261 gccgacaggt gccggcgcac gcagttgatt tcaccgatgg ccgcccccga cgcgagcagc
     7321 gcgcgattga cctgctgctt gtcggcaagc gagatacccg gcgcgggaag cgtgaggagc
     7381 gccgacccgc cgccggaaat gagcgcgacg acgagatcgt cgcgcgaaag accggcgatg
     7441 cttgcgagca cgcgccgtgc ggccgccacg cccgcggcgt cgggtaccgg atgcgcggcc
     7501 tcgacgacct cgatgtgctc gcatcgcacc gcgtgtccgt atcgcgtgac gacgatgccg
     7561 cgaagcgcac cgcgccagtg ctcctcgagc gcgcgcgcca tcgccgccga ggccttgccc
     7621 gcgccgacga ccagcgtgcg tccgcgcggc gggtcgggca gacagggcgg caatgcgtgc
     7681 gcaggttgcg cggattcgac cgcggcgtcg aacatgcgcc gcaacaacgt gcgctcgtcg
     7741 aggttcgcca tcagctcgct attgcgtgac gcggctatcg cgggtcggcg gagcccgcac
     7801 cgggccgctc gtcacccgcg cggcggccgc cggcggcggg catctttcgc aatgcgtccc
     7861 agcccggcga cggcccgaag cgcccgacga ggtaatcgac gaacacgcgc actttcgccg
     7921 cgagatagcg gttcggaaga tacgtcgcat aaagcgccgt ctccgcagcc ttgtactccg
     7981 gcagcacggt gcgcagcgtg ccctgctgca ggtcgccgcc gacgatgtag gtgggcagca
     8041 gcgcaatgcc gcagccggcg agcgcggcct ggcgcacggc attctcgttg tcgagccgga
     8101 gcgcgccgct cacctcgacg ctcgcgcgcc cgtcggcgct gtcgaaatgc caatggcgct
     8161 cgggcccgag gcgtgagaag acgatgcaat cgtgatgcgc gaggtcggca acggatgcgg
     8221 gtgcaccgtg acgccgcaga tacgcgggcg cggcgcacag cgcctggcga atgggcgcga
     8281 gcttgcgcgc aacgagcgtg gcgcgcggct cggcggcgat caggatcgcg acgtcgtacc
     8341 cctcctcggc caactgcacc tcgcgatcgt tcagcgtcat atcgacgtgc agctcggggt
     8401 acgtggcaag cagcccggcg attacgggaa cgatgtgcag cacgccgaac gacaccggca
     8461 cgttgacctt gagcgtgccg cgcggcgtcg actgcatgcc ggcggtcatc gcctccgcgt
     8521 cctcggccgc cgcgacgata tgcgcgcatc gcgcgtaaaa ctcgcggccg atctcggtga
     8581 ggctgatgct gcgcgtcgtg cgattgagca gccgcacgcc gagcgagcgc tccatgcgcg
     8641 tcacgcattt gctgaccacc gacgtcgaca cgttgagccg gcgcgcagca cccgagaaac
     8701 tgcgcgcttc gacgacgcgc gcgaatattt ccattccggc gagatccatg acggccgatt
     8761 gcgcttgttc tgcgaacaat gttattccga attcttgatg aacgcgaccg aacgccgcca
     8821 ccgctttttc catcgaagtt ggaacaaaca tcgcgcgaga cacgaatcgc acgctcgtgc
     8881 gcgatttttc aatcttgtcg gtgccgtccc acgacggttc gcgacgcgac acgcgattgg
     8941 cccgttcatg cgcttcatcc ccgcacgccg ccgaatcctg aaagccgccc tgatcccatc
     9001 ggcggcgagc gtcgtgccat tgcgcttcgt gcgcgatgct gcggccgagc cggtatcggc
     9061 ccccggacag tttgcgtact tcacgcccgt cgaattcacg tgggtcgacg ctgccgtcgc
     9121 gcgtctcatc cccaacgacg agctcgggcc cggtgcaaag gaagcgggcg tcgcgacatt
     9181 catcgaccgc cagctctccg gaccttacgg ccgtgccgag acctggtaca tgcagggccc
     9241 gtggaaggaa ggaacgaagg aacagggata tcagctcaag atgacgccgg cgcagctcta
     9301 ccgcacggcc attgccgacg tcgatgcatg gtgcagcagc agcggcaagc gcccgttcgc
     9361 cgagctcgaa ggcgctgcgc aggacaagct gctgcacgac ctggagaagg gcaacatcaa
     9421 gctcacgcgc gccccagcga aggacttctt ccagatgctg ctccagaaca ccgtggaagg
     9481 atttctcgcc gatccaatgt acggcggcaa tcgcgatttc gccggctgga agctgatcgg
     9541 ctttccgggt ccgcgctaca actacatcgc cgagatcgac cagtacggca agcgctatgc
     9601 gatgccgacc gtcggactcc tcggccgcga cggcacgcgc gtgaaggaaa cctgatggca
     9661 acgaaactgc cttccgtgga cgtcgtgctc gtcggcttcg gctggacggc gagcattatc
     9721 ggacaggagc tgaccgacgc gggcctgcag gtcgtggccc tcgagcgcgg cgcgttccgc
     9781 gacacggttc ccgatttcgc gacgacgcag atccaggacg agctgcgcta cgcggtgcgc
     9841 aacgagcttt tcgaggagcc ggagcgcgag acgctgacgt ttcgcaattc gatggaccag
     9901 accgcgttgc cgatgcgcca cctgggctcg ttcaagccgg cggccggcgc gggcggcgcg
     9961 ggcgtgcact ggaacggcca gaactggcgc ttcctgccga ccgatttcct gcagcgctcg
    10021 cacatcgagc agcgctacgg caagggcttc attccggccg acatgacgat ccaggactgg
    10081 ggcg
//
LOCUS       DASSVX010000019         9955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_233847, whole genome shotgun sequence.
ACCESSION   DASSVX010000019 DASSVX010000000
VERSION     DASSVX010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233847"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFO53_00960"
     CDS             complement(<1..423)
                     /locus_tag="VFO53_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET8874870.1"
                     /translation="MYRKILVAFDGSPESRLAVDECSNLMQLAGRQIHLVCVLHDPSP
                     YLLAGEFVPEPALAIDRSRMQADLDEAAARLTGRGFSVTTHLQDGEPVDVISRMVDSL
                     GIDLVIVGHRRSKNFALRWWRGSVDSMLVERVHCSILVA"
     gene            complement(426..1874)
                     /locus_tag="VFO53_00965"
     CDS             complement(426..1874)
                     /locus_tag="VFO53_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET8874871.1"
                     /translation="MRRPVGCARRHRRGALRRAATSRPLNTRAAATSSRALVVTTVML
                     GTISTILSATIVNVAFPALIDEFHVGHDTLQWVAAGYLAAMTATMLTTAWLVQTFGER
                     NAFVGMLGLFLAASLLAAASSSTDALIAARVLQGAAAGVLQPLSMIVLFRVFPVEERG
                     RAMALYGFGIVLAPAIGPAIGGTLLDAFGWRSIFLLTVPSSIAGLVLGLRTLQARSSM
                     QPVRFDAAGWLLLTAALIALMNVPVAGHRLGFASVEAVALLGGGLVLAAAFIGWEMRA
                     SSPLLPIGLFRHRPFAGATIVAFAYGIGLFGSTYLVPVFAQQIASYDASEAGYLLMPP
                     GIALAVAIAIGGRMSDRIEPAPIIIAGLALFALSSLLLAFSGAATGFVLLALWLVIGR
                     VGLGMLIPALNVAAVQSLSGTELAYASAAVNFVRQLGGAIGVNLLAVVLEWRLDAPGV
                     EPARAFHECFVIVTLAFAFAIVPAWFIRSQRR"
     gene            complement(1799..2821)
                     /gene="holA"
                     /locus_tag="VFO53_00970"
     CDS             complement(1799..2821)
                     /gene="holA"
                     /locus_tag="VFO53_00970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET8874872.1"
                     /translation="MQVRPHELDAHLSRKLAPLYVVHGDEPLAAIEAADAIRAAARRA
                     GCDERELFIVEQHFRWDAFVAANANLGLFASRKLVDVRIASGKPGTEGAAALERHAQN
                     LPADNVTLISLPRVDRTTQASAWFSALAEHGVMIGVTPVDRAALPAWIAGRLARNDQR
                     VTRETLAYLAERCEGNLLAAKQEIEKLALLLAPGQLGHDDVERAVANVARYDIQELSE
                     AWLRGEAARTLRIVDGLRSEGEPITLVAWQLGEDLHALAAVHEMMAHGQSLSSAMRNV
                     RVWGKRQPALERAARRLDASRVLPLLASLARLDALAKGIGCGDPWDALVAIAVALCEE
                     PPPAAP"
     gene            complement(2942..3457)
                     /gene="lptE"
                     /locus_tag="VFO53_00975"
     CDS             complement(2942..3457)
                     /gene="lptE"
                     /locus_tag="VFO53_00975"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HET8874873.1"
                     /translation="MKPTRSTSVARAIVAAIAVALALAACGFHLRGEATYTFATIYVN
                     APGAPGLAVELKRALNATGSAKVVDDPKNAQVTLDIPLVADDKEVLSISGAGAVQEYA
                     LIKRVGFRLHDADGNDWLPAAEISLRRSYTFSESEVLARGTQEERLLKEMQTDAVQQL
                     IRRLQAAKKPA"
     gene            complement(3489..6134)
                     /gene="leuS"
                     /locus_tag="VFO53_00980"
     CDS             complement(3489..6134)
                     /gene="leuS"
                     /locus_tag="VFO53_00980"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET8874874.1"
                     /translation="MTPFEPAAVESTAQAAWKAGDVYRAVENDPRFRCGKFFCVSMLP
                     YPSGKLHMGHVRNYTINDMMYRQLRMRGYNVLMPMGWDAFGLPAENAAMKSREAPARW
                     TRDNIAYMKKQLQAMGFAIDWSREFATCDPAYYRWNQWFFLRMREAGIAYRKNGTVNW
                     DPVDQTVLANEQVIDGRGWRSGAPVEKREIPMYYLRITEYADELLSELDRMPGWPERV
                     KTMQANWIGKSDGVRFAFAHAIGDESGAPIGDGQLYVFTTRADTIMGVTFCAVAADHP
                     LAAHAARTNPALAAFIDECKRGSVIEADHATMEKKGMRTGLTVTHPLTGAPIDVWVGN
                     YVLMSYGDGAVMGVPGHDERDFEFAKKYGLAIRQVIAPAEPSALPAFTTERWQPWYAD
                     KTIGVCIHSGRYDGLAYPAAVDAIATDLARLGLGEKRTTWRLRDWGISRQRYWGTPIP
                     IIHCAHCGEVPVPEQDLPVVLPEDCIPDGSGNPLNRREDFLNVACPSCKAPARRETDT
                     MDTFVDSSWYFMRYTSPGAATMIDARDRYWMPMDQYIGGITHAILHLLYARFWTKIMR
                     DFGMVGYNEPFTRLLTQGMVLAQAFYRRNGKGGLDYVPPSDVEVARDASGTITGGISK
                     IDGQPVEYDGLGTMSKSKLNGVDPQDMIDRYGADAARLFVMFASPPEHTIEWSDTGVD
                     GAHRFLKRLWNHAQSQRDVVAAASGAFDWRDAAETVRTARRDIHLALKQADYDYERIQ
                     YNTVVSAGMKMLNTLEDVPGDAAGATALAREGLSILLRVLYPVVPHTTWALWRELRFT
                     DAYGDLIDAPWPCVDGSALARDEIELVLQVNGKLRGKLVVPAGADRAAIEAAARDAPE
                     VARHGGGGEIRKVIVVPGRLVNVVV"
     gene            6309..8453
                     /locus_tag="VFO53_00985"
     CDS             6309..8453
                     /locus_tag="VFO53_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET8874875.1"
                     /translation="MRVIRQFRSYLAALVAAFAVLGAAHAAPVRTGHVTAELVSDQAA
                     LVPGTTTTLALRLAIERGWHTYWRNPGESGLPTTLDWHLPPGYAAGDIAWPAPRALPA
                     GPLMNYGYEGEVFHLVPVTVPASAAPGANVALAARADWLVCKETCIPEGADLTLELPV
                     AKAAEPSRWHDAIAATRAALPGPLPAGWQARATASGAVVTLTLDAPANAADPGRLRFF
                     AVDERRIEPSATQKRVASADGSYALQLPVSHELSGDFARLQGVLRADKGIGGDGRLVG
                     AVALDLPLAGTPAAGPKPVQVATPDLTQPAPSGAVALSLPLALAFAFGGGLLLNLMPC
                     VFPILSLKALGLATSPADDRRALRHDGVAFAAGVVLAFAALGIALATLRAAGAELGWG
                     FQLQSPAVVTSLAILFFVMAQNLSGVFEFGSLVPSRIATWSHANRQVNAFASGILAVA
                     IASPCTAPFMGAALGYALGERAQVTLGVFVALGLGMALPYVLLAWFPQWRRVLPRPGA
                     WMQRLKQLLAFPLYATVAWLVWVLAAQVGTDAVIRIGVVLVLVAFALWAWRTWRVAAH
                     RAFRAAAVASAIAGLLVAWPLFAGGRAVADTPAAQAADGWQAYSPASIAELTAEGRPV
                     FVDFTAAWCITCQVNERFVLNDALVREAFARSNVALVRADWTRRDGDITEALAALGRS
                     GVPAYVIYRKGRAPQLLPEVLRRQTVIDALAS"
     gene            complement(8537..9322)
                     /locus_tag="VFO53_00990"
     CDS             complement(8537..9322)
                     /locus_tag="VFO53_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET8874876.1"
                     /translation="MNCADASTLLSAYTDGETSMLQGHSVKRHLQSCANCAAAYDDML
                     ALRARLRAEAPRHAAPPELRARVLATLDAVRASTPPPARPRATSERWRWLSGGALAGC
                     TATALAWFIGTAVIDWRANEDLAVEAVTMHVHATLGNQQIQVASSDRHTVKPWLSARL
                     DYSPPVRDFAQDGFPLVGGRIDYLDRHPVATLVYRVRNHTIDVYVRPETASRVPPSAL
                     RTIRGFNVAHATGSGMDWLAVSDVNADELTGFVQKLARESGAP"
     gene            complement(9319..9858)
                     /locus_tag="VFO53_00995"
     CDS             complement(9319..9858)
                     /locus_tag="VFO53_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET8874877.1"
                     /translation="MSDHTTFEQLVLPHLDAAYNLARWLARDTHDAEDVVQDACIRAL
                     KYARSLHGNDARAWFLTIVRNAFYDWLGRNRPAEIVHEDDDAIAARIDPSSPDPELEA
                     LRRADMHSLSDAIEALPLQYREVLVLRELEEMSYKEIARVADIPIGTVMSRLARARAL
                     LQRSPLLQAIGGQATGSER"
BASE COUNT         1517 a         3407 c         3440 g         1591 t
ORIGIN      
        1 cgcgacgagg atgctgcaat gcacgcgctc gacgagcatc gaatcgaccg agccgcgcca
       61 ccagcgcagc gcgaagttct tgctgcgccg gtgaccgacg atgacgaggt cgatgccgag
      121 cgaatcgacc atccgcgaga tgacgtcgac cggctcgccg tcctgaagat gcgtggtcac
      181 cgagaagccg cgtccggtca ggcgcgcggc cgcctcgtcg aggtcggcct gcatccgcga
      241 gcggtcgatg gcgagtgcgg gctccggcac gaactcgccg gccagaaggt acggcgacgg
      301 atcgtgcagc acgcacacga gatgaatctg ccgtccggcg agctgcatca ggttcgagca
      361 ttcatcgacg gcgaggcggc tttcgggcga gccgtcgaac gcgaccagga tcttccggta
      421 catggctatc tccgttgact gcgaatgaac cacgcgggca cgatggcgaa cgcaaacgcc
      481 agcgtaacaa tcacgaagca ttcgtggaag gccctcgccg gctcgacgcc cggcgcatcg
      541 agccgccatt cgaggaccac ggcgagcaag ttcacgccga tcgcgccgcc gagctggcgc
      601 acgaagttca ctgcggccga cgcataggcg agctcggtgc ccgacagcga ctgcaccgcg
      661 gcgacgttca gcgcgggaat cagcatgccg aggccgacgc ggccgatcac gagccacagc
      721 gcaagcagca cgaagccggt ggccgcaccc gagaacgcga ggagcaggct cgacagcgcg
      781 aacagcgcca gcccggcgat gatgatcggc gcgggctcga tgcggtcgct catgcgcccg
      841 ccgatcgcga tcgcaacggc cagcgcgatg ccgggcggca tcagcagata gccggcctcc
      901 gaggcatcgt agctcgcgat ctgctgcgcg aacacgggca cgagatacgt gctgccgaag
      961 aggccgatcc cgtaggcgaa tgcgacgatc gtcgcgccgg cgaacgggcg atgacgaaag
     1021 aggccgatcg gcaatagcgg cgaactcgcg cgcatttccc agccgatgaa tgccgccgcg
     1081 agcaccagcc cgccaccgag aagcgctacc gcctcgacgg aggcgaatcc cagccggtgc
     1141 ccggcgacgg ggacgttcat cagcgcgatc agcgccgcgg tgaggagcag ccatcccgcc
     1201 gcatcgaagc gcaccggctg catcgacgag cgtgcctgca gcgtgcgcag accaaggacc
     1261 agtcccgcga tgctggacgg cacggtcagc agaaagattg agcgccagcc gaacgcgtcg
     1321 agcagcgtgc cgccgatggc gggtccgatc gccggtgcca ggacgatgcc gaagccgtaa
     1381 agcgccatgg cgcgtccgcg ttcctcgacc gggaacacgc ggaacagcac gatcatcgac
     1441 aagggctgca gcaccccggc ggccgccccc tgcagcacgc gcgcggcgat cagcgcgtcg
     1501 gtgctcgagc ttgccgcggc gagtagcgat gccgcgagga agaggccgag catcccgacg
     1561 aacgcgttgc gctcgccgaa cgtctgcacg agccacgcgg tggtgagcat cgtggcggtc
     1621 attgccgcga gatagccggc cgcgacccac tgcagcgtgt cgtgcccgac gtgaaactcg
     1681 tcgatcagcg ccggaaatgc gacgttgacg atcgttgccg aaagaatcgt cgagatcgtc
     1741 ccgagcatca ccgtcgtgac gacgagtgcg cgggaggacg tcgcggcggc gcgcgtgttc
     1801 agggggcggc tggtggcggc tcttcgcaaa gcgccacggc gatggcgacg agcgcatccc
     1861 acgggtcgcc gcacccgatg cccttggcga gggcgtcgag cctcgcaagc gacgcgagaa
     1921 gcggcagcac acggctcgca tcgaggcgcc gcgcggcgcg ttccagcgcc ggctggcgct
     1981 tgccccagac gcgcacgttg cgcatcgcgc tcgacagcga ctgcccgtgc gccatcattt
     2041 cgtgcacggc cgcgagcgca tgcaggtctt cgccgagctg ccaggcgacg agcgtgatcg
     2101 gctcgccttc gcttcgcaag ccgtcgacga tgcgaagcgt ccgcgccgct tcaccgcgca
     2161 accaggcttc ggacagctcc tggatgtcgt agcgggcgac attcgcgacc gcgcgctcga
     2221 cgtcgtcgtg cccgagctgc cccggcgcca gcaagagcgc gagcttttcg atttcctgct
     2281 tcgccgccag caggtttccc tcgcagcgct cggcgagata ggccagcgtc tcgcgcgtga
     2341 cgcgctggtc gttgcgcgcc agtcgcccgg cgatccatgc cggcagtgcc gcgcggtcga
     2401 ccggcgtcac gccgatcatc actccgtgct cggcgagagc cgaaaaccag gccgacgcct
     2461 gcgtggtgcg atcgacgcgc ggcagcgaga tcagcgtgac gttgtcggcg gggaggtttt
     2521 gtgcatgccg ctcgagtgcg gcggcgcctt ccgtgccggg cttgccggac gcgatgcgca
     2581 cgtcgacgag cttgcgcgac gcgaagaggc cgaggttggc gttggccgca acgaacgcat
     2641 cccagcgaaa atgctgctcg acgatgaaga gctcgcgctc gtcgcaaccg gcgcgccgcg
     2701 cggcggcgcg gattgcgtcc gccgcctcga ttgccgcgag tggctcgtcg ccatgaacga
     2761 catagagggg tgccaacttg cgcgaaagat gcgcgtcgag ctcatggggg cggacttgca
     2821 tggctagtgg tccccccacg cttgcagctt cgctgcggcg ctgcccccgt agggggcgct
     2881 gttcggggct tgggacggcc cgtcgcccct cacggtcatg atctccgacg gtccggcgcg
     2941 ttcacgccgg ctttttcgcc gcctgcagac ggcgaatgag ctgctgcacg gcgtcggtct
     3001 gcatctcctt cagcaggcgc tcctcctgcg tgccgcgcgc aagcacctca ctttcgctga
     3061 acgtgtagct ccggcgcagc gagatctcgg ccgccggcag ccaatcgttg ccgtcggcgt
     3121 cgtgcagcct gaagccgacg cgcttgatca gcgcatattc ctggacggcg cccgcgccgg
     3181 agatcgacag cacttccttg tcgtcggcga cgagcggaat gtcgagagtc acctgcgcgt
     3241 tcttgggatc gtcgacgacc tttgcgctgc ccgtcgcgtt gagcgcacgc ttcagctcca
     3301 ctgcgagccc cggtgcgccg ggcgcgttca cgtagatcgt cgcgaaggtg taggtggcct
     3361 cgccgcgcag gtgaaagccg cacgcggcaa gcgcaagcgc aacggcgatc gcggcgacga
     3421 tggcacgcgc aacggatgtc gaacgcgttg gcttcatgga cgaagcttcc tcgacgcgcg
     3481 tgcgtgcctc agacgacgac attgacgagc cgcccgggga cgacgatgac ctttctgatt
     3541 tcgccgccgc caccgtggcg cgcgacctcg ggcgcgtcgc gcgcggccgc ttcgatcgcg
     3601 gcgcggtcgg cgccggcggg cacgacgagc ttgccgcgaa gcttgccgtt cacttgcagc
     3661 acgagctcga tctcgtcgcg cgcgagcgcc gacccgtcga cgcacggcca tggcgcatcg
     3721 atgagatcgc catacgcgtc ggtgaaccgc agctcgcgcc acagcgccca ggtcgtgtgc
     3781 ggcacgaccg gatacaacac gcgcagcagg atcgacaagc cttcgcgggc gagcgccgtg
     3841 gcgcccgctg cgtcgcccgg cacgtcctcg agcgtgttca gcattttcat tccggccgac
     3901 accacggtgt tgtactggat gcgctcgtag tcgtaatcgg cctgcttcag cgcgagatgg
     3961 atgtcgcggc gcgcggtccg gaccgtttcc gcggcatcgc gccaatcgaa agcgccgctc
     4021 gccgccgcaa cgacatcgcg ctgcgactgc gcgtggttcc acagccgctt cagaaaccgg
     4081 tgcgctccgt cgacgccggt gtcggaccat tcgatcgtgt gctcgggcgg acttgcgaac
     4141 atcacgaaca ggcgcgcggc gtcggcgccg taacggtcga tcatgtcctg cgggtcgacg
     4201 ccgttgagct tggacttcga catcgtgccg aggccgtcgt actcgaccgg ctgtccgtcg
     4261 atcttcgaaa tgccgccggt gatcgtgccg ctcgcatcgc gcgcgacttc gacgtcggat
     4321 ggcggcacgt aatcgaggcc gcccttgccg ttgcgccggt agaacgcctg cgcaaggacc
     4381 atcccctgcg tgaggagccg cgtaaagggc tcgttgtagc cgaccatccc gaagtcgcgc
     4441 atgatcttcg tccagaaccg cgcgtagagg aggtgcagga ttgcgtgcgt gatgccgccg
     4501 atgtactggt ccatcggcat ccagtagcgg tcgcgcgcgt cgatcatcgt tgcagcaccg
     4561 ggcgacgtgt aacgcatgaa gtaccacgac gagtcgacga acgtgtccat cgtgtcggtt
     4621 tcgcggcgcg cgggcgcctt gcagctcggg cacgcgacgt tcaggaagtc ctcgcgacga
     4681 ttcaacggat tgccgctgcc gtcgggaatg caatcctcgg gcagcacgac cgggagatcc
     4741 tgctcgggca ccggcacttc gccgcaatgc gcgcagtgga tgatcgggat cggcgtgccc
     4801 caatagcgct ggcgcgagat gccccagtcg cgaaggcgcc acgtcgtgcg cttttcgccg
     4861 aggccgagcc gggcgagatc ggtggcgatc gcgtcgacgg ccgccgggta cgcaagcccg
     4921 tcgtacctgc ccgagtggat gcagacgccg atcgtcttgt cggcgtacca cggctgccag
     4981 cgctcggtcg tgaaggcggg caatgccgat ggctcggccg gcgcgatcac ctggcggatc
     5041 gccagcccgt actttttcgc aaactcgaag tcgcgctcgt cgtgacccgg cacgcccatc
     5101 acggcgccgt cgccgtagct catcagcacg tagttgccga cccacacgtc gatcggtgca
     5161 ccggtcagcg gatgcgtgac ggtgagtccc gtccgcatcc ccttcttctc catggtcgcg
     5221 tggtcggcct cgatcaccga tccgcgcttg cactcgtcga tgaacgcggc cagcgccggg
     5281 ttcgtgcgcg cggcgtgcgc ggccagcgga tggtcggctg cgaccgcgca gaacgtcacc
     5341 cccatgatcg tgtcggcgcg cgtcgtgaac acgtaaagct ggccgtcgcc gatcggcgcg
     5401 ccggattcgt cgccaatggc atgcgcaaag gcgaagcgca cgccgtcgct cttgccgatc
     5461 cagttcgcct gcatcgtctt cacgcgctcg ggccagccgg gcatgcggtc gagctcggac
     5521 agcagttcgt cggcgtactc cgtgatgcgc aggtagtaca tcgggatctc gcgcttttcc
     5581 accggcgcgc ccgagcgcca cccgcgcccg tcgatcacct gctcgttcgc gagcaccgtc
     5641 tggtcgacgg ggtcccagtt gacggtgccg ttcttccggt aggcaatgcc cgcctcgcgc
     5701 atcctgagga agaaccactg gttccagcga tagtaggcgg ggtcgcaggt cgcgaactcg
     5761 cgcgaccagt cgatcgcgaa gcccatcgcc tgcagctgct tcttcatgta cgcgatgttg
     5821 tcgcgcgtcc agcgcgcggg cgcctcgcgg cttttcatcg ccgcgttctc ggcgggcagg
     5881 ccgaatgcat cccagcccat cggcatcagc acgttgtaac cgcgcatgcg cagctgccgg
     5941 tacatcatgt cgttgatcgt gtagttgcgc acgtgaccca tgtgcagctt gccggacggg
     6001 tagggcagca tcgacacgca aaagaacttg ccgcagcgaa agcgcgggtc gttctctacg
     6061 gcgcggtaga cgtcgcccgc tttccaggcg gcctgcgcgg tgctttcgac ggcggcgggc
     6121 tcgaacggcg tcatggcgcg aaaatgcgga atgcgcgggc ggtgcgcgct gttgtgtgta
     6181 agtaattgat tataccggcg accaccgcgc aaaccggtag acggggtccg cagtggcaca
     6241 atggcaggtt gcgccccctg gccggcaccg tccgtcgctt cgttgcaata tcgatcactt
     6301 cgacgcccat gcgcgtgatt cgccaatttc gctcgtacct cgcagcactc gttgccgcgt
     6361 tcgcggtgct cggcgccgcg cacgcggcgc ccgtgcgaac cggccatgtg acggccgagc
     6421 tcgtttccga tcaagcggcg ctcgtgccgg gaacgacgac gacgctcgcg ctgcgcctcg
     6481 cgatcgagcg cggctggcac acctactggc gcaatccggg tgaatcggga ctgccgacga
     6541 cactcgactg gcacctgccg cccggctacg ccgcgggcga cattgcctgg cccgcaccgc
     6601 gtgcgctgcc cgcgggaccg ctgatgaact acggttacga aggcgaggtg ttccacctcg
     6661 tgcccgtcac ggttcccgcc agcgcggcgc cgggcgccaa cgtcgcgctc gccgcacgcg
     6721 ccgactggct ggtctgcaag gaaacgtgca ttcccgaggg cgccgacctg acgctcgagc
     6781 tgccggtggc aaaggctgcc gagccgtcgc gctggcacga cgcgatcgcc gcgacgcgcg
     6841 cggcgctgcc cgggccattg ccggcggggt ggcaggcgcg cgcgacggct tccggcgctg
     6901 tcgtcacgct cacgctcgat gcgccggcga acgcggccga tcccggacgc ctgcggttct
     6961 tcgcggtcga cgagcggcgc atcgagccct cggccacgca gaagcgcgtc gcctcggcgg
     7021 atggcagcta tgcgctgcag ttgccggtgt cgcatgagct gagcggcgat ttcgcacgcc
     7081 tgcagggggt gcttcgcgcc gacaagggca tcggaggcga tggtcgcctg gttggcgccg
     7141 tcgcactcga ccttccgctc gccggcacgc cggctgccgg gccgaagccc gtgcaggtgg
     7201 cgacgccgga cctcacgcag cccgcgccct ccggcgccgt cgccttgtcg ttgccgctgg
     7261 cgcttgcatt cgccttcggc ggcggcctgc tgctgaacct gatgccgtgc gtgttcccga
     7321 tcctgtcact caaggcgctg ggtctcgcga cgtcgcccgc cgacgaccgg cgcgcgctgc
     7381 gccacgacgg ggttgcgttc gccgcaggcg tggtgctcgc gttcgcggca ctcggcattg
     7441 cgctcgcaac gctgcgtgcc gcgggtgcgg aactcggatg gggcttccag ttgcaatcgc
     7501 cggccgtggt cacgtcgctt gcgatcctgt tcttcgtgat ggcgcagaac ctgtccggtg
     7561 tgttcgagtt cggctcgctc gtgccgtcgc gcatcgcgac ctggtcgcac gcgaaccggc
     7621 aggtgaacgc gttcgcctcg ggcattctcg cggtcgccat cgcgtcgccg tgcacggcgc
     7681 cgttcatggg ggcggcactc ggttatgcgc tcggcgagcg cgcacaagtc acgctcggcg
     7741 tgttcgtcgc actcggcctc ggcatggcgc tgccctacgt gttgcttgcg tggttcccgc
     7801 agtggcggcg cgtgctgcca cgaccgggcg catggatgca gcgcctgaag caactgctcg
     7861 cctttccgct gtatgcaacc gttgcgtggc tggtctgggt gctcgccgcc caggtgggca
     7921 ccgacgcagt gatccgcatt ggcgtcgtgc tcgttctcgt ggcgttcgcg ctctgggcgt
     7981 ggcgcacctg gcgcgtggcg gcgcaccgcg cctttcgcgc cgccgcagtc gccagtgcca
     8041 ttgcgggcct gctggtggcg tggccgctct ttgccggagg ccgcgcggtt gcggataccc
     8101 cggcagcgca agcggccgat ggctggcagg cgtattcgcc ggcaagcatt gcggaactga
     8161 ccgccgaggg ccggccggtg ttcgtcgatt tcaccgccgc ctggtgcatc acctgccagg
     8221 tcaacgagcg cttcgtgctg aacgatgcgc tcgtgcgcga ggcgttcgcg cgcagcaacg
     8281 tggcgctggt gcgcgccgac tggacgcggc gcgacggcga catcaccgaa gcgctggcag
     8341 cgctcggccg cagcggcgtg ccggcctacg tgatctatcg caagggccgc gcgccgcaat
     8401 tgctgcccga ggtcctgcgg cggcagacgg tcatcgacgc gctcgcgtcg tagcatcgtc
     8461 ggcgaaaccg cgccggggcg tgcgtggcga tcctgcgcac cgcggcgtgc cgaccgccta
     8521 cggcgcgcgg aactggctac ggtgcgcccg actcgcgcgc gagcttctgc acgaaaccgg
     8581 tgagctcgtc tgcattcacg tcggacaccg cgagccagtc catgcccgac ccggtcgcat
     8641 gcgcaacgtt gaacccgcgg atcgttcgca acgccgacgg cggcacgcgc gacgccgtct
     8701 cgggacgcac gtacacgtcg atcgtgtgat tgcggacgcg atagacgagc gttgccaccg
     8761 gatgccggtc gagatagtcg atgcgcccgc cgaccaatgg gaatccgtcc tgcgcgaagt
     8821 cgcgcaccgg cggcgaatag tcgaggcgcg cggaaagcca gggcttcacg gtgtgccgat
     8881 cggacgacgc gacctggatt tgctgattgc cgagagttgc atgcacatgc atcgtcaccg
     8941 cctcgaccgc gagatcctcg ttggcgcgcc aatcgatcac cgccgtgccg atgaaccacg
     9001 cgagtgccgt cgccgtgcat ccggcgagcg cacctcccga aagccagcgc cagcgctcgc
     9061 tcgttgcgcg gggtcgcgcc ggcggaggcg tcgatgcgcg taccgcgtcg agcgtcgcga
     9121 gcacgcgagc gcgtagctcg ggcggtgcgg catggcgcgg cgcttccgcg cgcaaccgcg
     9181 cacgcaaagc gagcatgtcg tcgtaggcgg ccgcgcagtt cgcgcagctt tgcagatggc
     9241 gcttgaccga atggccctgc agcatgctgg tttcgccatc ggtgtaggcc gaaagcaggg
     9301 tcgacgcatc agcgcagttc atcgttcgct cccggtagcc tgcccgccga tcgcctgcaa
     9361 caggggcgag cgctggagca gcgcgcgcgc ccgtgcgagg cgcgacatga cggtgccgat
     9421 cggtatatcg gcgacccgcg caatttcctt gtacgacatt tcttccagct cacgcagcac
     9481 gagcacctcg cggtattgca gcggcagcgc ctcgatggcg tccgacagcg agtgcatgtc
     9541 cgcgcgccgc agcgcctcga gctcggggtc gggcgatgac gggtcgatgc gggcggcgat
     9601 cgcgtcgtcg tcctcgtgca cgatttccgc cggccggttg cggccgagcc agtcatagaa
     9661 cgcgttgcgc acgatcgtga ggaaccacgc gcgcgcgtcg ttgccgtgca gcgagcgggc
     9721 gtatttgagc gcccggatgc acgcgtcctg gaccacgtcc tcggcgtcgt gcgtatcgcg
     9781 agcgagccag cgcgcgaggt tgtacgcggc atcgaggtgc ggcaagacca gctgctcgaa
     9841 cgtggtgtga tcggacaagc gcgtctccct gttgccctga cataccggga acgatcccgg
     9901 cttattccag cgcgcggccg cgtccggaac gcggcggcgg tgtgctgcag gcgat
//
LOCUS       DASSVX010000020         7338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_533890, whole genome shotgun sequence.
ACCESSION   DASSVX010000020 DASSVX010000000
VERSION     DASSVX010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533890"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..964)
                     /locus_tag="VFO53_01000"
     CDS             complement(59..964)
                     /locus_tag="VFO53_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8874878.1"
                     /translation="MSKQMEARLSPGRNEPKPPFPKQHQEGPGIESKLDPRPRYKAEA
                     YRPAGKLEDKVALITGGDSGIGRAVALLYAREGARVAITYLPEEQSDADDTRKAVEGV
                     GGQCLAIAGDLTEPAFCDKVVEETVQRFGKLDILVANAGHQNRKQTLQDVTDDEFDRT
                     FKTNIYSYFRLSRAALRHMQPGSAIIATSSETGIFGSKYLPDYSATKGAINAFTKTLA
                     MNLVDKNIRVNAVAPGPVWTPLNPSDAGETPDRVAHFGEDSPMGRPAQPEELAPAYVF
                     LASNADSSYITGIVLQVMGGETTGG"
     gene            1120..1623
                     /gene="lysM"
                     /locus_tag="VFO53_01005"
     CDS             1120..1623
                     /gene="lysM"
                     /locus_tag="VFO53_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HET8874879.1"
                     /translation="MGLMDFVKDAGEKLFGHGQAQAAVKEAAADPSSQAKVQAANSAA
                     ADAILEYVKSQHLTATGLTVTYDGATSTVSVFGVAPDQATREKIVLCCGNVAGVAKVN
                     DMMSVDRSEPEAQFYTVVSGDNLSKISKQFYGDANRYMKIFDANKPMLTSPDRIYPGQ
                     KLRIPPQ"
     gene            1713..2009
                     /locus_tag="VFO53_01010"
     CDS             1713..2009
                     /locus_tag="VFO53_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874880.1"
                     /translation="MLAAHGDAFVAETLGRCLGLGPGDDDDDDDPVPIGDPPDDDEGD
                     DQDDDDEDEDDEEPMQVRRCASPYRQRARITAQCRPDDRSRAAVPVARYPIPDL"
     gene            2066..3055
                     /locus_tag="VFO53_01015"
     CDS             2066..3055
                     /locus_tag="VFO53_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8874881.1"
                     /translation="MLSEVTLDVARGELVALLGSSGCGKTTLLRSIAGFVTPQSGRIR
                     IAGRDITGLPPEKRGTAMMFQSYALWPHMSVAGNIGYGMRMRGAARGAIARRVDELLA
                     LMQLQGYAARSVVQLSGGQRQRVALARALAVDPALLLLDEPMSNLDYKVRIELRHELR
                     ALQKRIGITAVYVTHDREEALTLADRIAVLDAGRVVQYGSPEEIFHRPTSAFVAGFMG
                     ADNAIELRRDEQGRLRSAAGNGDAGEPVTAHFRSDVARIDASAAAAGDAELRLQGQIV
                     QTLYVGQGYRYRVRTDGADVWAHASDRINEGAPVAVVVPREALLLFPRATTQG"
     gene            3108..4250
                     /locus_tag="VFO53_01020"
     CDS             3108..4250
                     /locus_tag="VFO53_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8874882.1"
                     /translation="MKVRSLLSIAALGLASIAPAAAQTTLNVVSAGDQNMVDYVNNYL
                     GPKFEQMNPGVKVRAVGTGPGDSGSQKIYEKLEAQQKAGSATWDVDVAVVHQRGAATM
                     VNGKLLMPYRNDVAAGKLVSRDTAKNALGANVDGYVLPMFHSQIAFAYNPDLVKAPPK
                     SFAELAEWVKKNPKQFGYNGVKGGMSGVGFVMGWVEANSGMRDKLEKGPYDPSAKATI
                     DKSLESLKAFNQYVVMTPGNAGTLDMLNRGEIAMGPVWVDMFYTWMADGKMNPKVKLE
                     LPAPGLPGQPMYYVIPAKAANAALAKKFIDFAESPEVQADGIVKQFNWYPGIDPQYVQ
                     SKMTPAAWNKLFTDISPSDLASKGRPFPLSDYFTDIVEGYERVVLK"
     gene            4288..5163
                     /locus_tag="VFO53_01025"
     CDS             4288..5163
                     /locus_tag="VFO53_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8874883.1"
                     /translation="MAAIAEEALVEAQPGGGGRWAALALVAPALLVVGLFFVVPLVLS
                     AVGAFRTAGGGFTLAHFAKALDLYGNDLIFTVEIVALSTLLIALIAIAIAGYLTLGEH
                     PRAVAVLRWLYRWPLFIPFVVAGQIMRTFLAKNGMLNHVLIGTGLIEPISAQSMLDWR
                     GIVIAFVWKQAPFVTLLLAGAMASLERQHIEAARNLGAPRLRVLVDIVLPQVRGTLLV
                     GLVLSFVTMLSVLSVPLMINPNSPTMITVDVAYRINTFADYAVANALCLLSLLLAAFG
                     AAFYLRHAVRRVEAG"
     gene            5160..5978
                     /locus_tag="VFO53_01030"
     CDS             5160..5978
                     /locus_tag="VFO53_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006468960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8874884.1"
                     /translation="MSTMAARLLPNAVRALVLALLAFAIFGPLLNLVLWAFAEKWYFP
                     NKLPLEYGFTFWQRVFSPRGNAIGSLGTSIWIAVLTVAVSLAVAVPAGYALGRLKLPW
                     RGLILLAFLLPQAVPNLPVYVNIAQVFYRIGLAGTITGVVLVHASHGLVLAVWIASAA
                     FASVDVSLEEAARNMGASAWTCFRTVTLPLAAPGLMASAIFVFLESVDEFTGTYFVGV
                     PDVTTLPLLMFNASMGGNYQIASITALLLLVPSIGFMLVVERFLKADVLAMVGR"
     gene            complement(5983..6381)
                     /locus_tag="VFO53_01035"
     CDS             complement(5983..6381)
                     /locus_tag="VFO53_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874885.1"
                     /translation="MTQPRHTRPPKGETRQEQTIDRMVEDSFPASDATQLPGRAAGAP
                     EPGAEQRDPKHEAPRKAPHTIGNQGVAPSTRMREESVSLGGSAVVTLRFDTEARRLSL
                     RFGEEGMALDANALDRLIGALSKKRAQMGE"
     gene            7008..>7338
                     /locus_tag="VFO53_01040"
     CDS             7008..>7338
                     /locus_tag="VFO53_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET8874886.1"
                     /translation="MRFEELKLMQPLLQALAECGYDEPTPIQAQTIPVLLAGRDVIAS
                     AETGTGKTAAFILPALQRLSEQGVRPAPKRRGAGVPRVLVLAPTRELAQQVATQSSRY
                     GQNLRLRT"
BASE COUNT         1164 a         2439 c         2441 g         1294 t
ORIGIN      
        1 aggcgagcgc ggtctgtgtc ctgactgcgt cgcgaatgcg tctctgaccc caatgcgctc
       61 atccgcccgt cgtctccccg cccatcacct gcaggacgat gccggtgatg tacgacgaat
      121 cggcgttcga cgcgaggaac acgtacgcgg gtgcgagctc ctcgggctgc gccggccgtc
      181 ccatcgggct gtcctcgccg aaatgcgcga ccctgtcggg cgtctcgcct gcgtcggacg
      241 gattgagggg cgtccacacc ggtcccggcg ccaccgcgtt gacgcggatg ttcttgtcca
      301 ccagattcat cgccagggtt ttcgtgaacg cattgatcgc gcccttcgtc gccgagtagt
      361 cgggaaggta cttcgagccg aagatgccgg tttccgagct cgtcgcgatg atcgcgctgc
      421 cgggctgcat atggcgcaac gccgcgcgcg acaaccggaa gtacgagtag atgttcgtct
      481 tgaacgtgcg gtcgaactcg tcgtcggtca cgtcctgcag cgtctgcttg cgattctggt
      541 gcccggcatt cgccaccagg atgtcgagct tgccgaaacg ctgcacggtc tcttcgacga
      601 ccttgtcgca gaacgccggc tcggtgagat cgccggcgat ggcaagacat tggccgccca
      661 cgccttcgac cgccttgcgc gtgtcgtcgg catcggactg ctcttccggc agataggtga
      721 tagcgacccg ggcgccctcg cgtgcataca gcagcgcgac ggcgcggccg atccccgaat
      781 cgccgccggt gatgagggcg accttgtcct cgagcttgcc ggccgggcgg tacgcctccg
      841 ccttgtagcg gggacgcgga tcgagcttcg attcgatacc cggtccctcc tgatgctgct
      901 tcggaaacgg cggcttcggt tcgttccggc cgggcgaaag gcgggcttcc atctgcttgc
      961 tcatcgcgtc tctcctgcaa aaattacctg cgtgcgccga tcgaggcgtg cagcgaaaat
     1021 cggcaggtcc ggggtgtatg ctgccgaacg ggcgcgcccg acgccgctac ggcccgttgg
     1081 accgcgcgat ttcgaattca atcgacgagg caggcaacca tgggattgat ggacttcgtg
     1141 aaggacgcgg gtgagaagct gttcggacac gggcaggcgc aggcggcggt gaaggaggcc
     1201 gcggccgatc cgtccagcca ggccaaggtg caggcggcga attcggcggc cgccgatgca
     1261 atcctggaat acgtcaagtc gcagcacctg acagcgacgg gcctgacggt tacgtacgac
     1321 ggtgccacgt cgaccgtcag cgtgttcggc gtcgcgcccg atcaggcgac gcgcgagaag
     1381 atcgtgcttt gctgcggcaa cgtcgccggc gtcgccaagg tgaacgacat gatgagcgtc
     1441 gaccgcagcg agcccgaggc gcagttctac acggtggtct cgggcgacaa cctgtcgaaa
     1501 atctcgaagc agttctacgg cgatgcgaac aggtacatga agattttcga cgccaacaag
     1561 ccgatgctga catcgcccga caggatctac cccgggcaga agctgcgcat tccgccgcaa
     1621 tgagcgctgc agagccgcct caaggcgcga attgcgcccc ctcggggggc agcgcagcgg
     1681 cgaagccgca agcgtggggg gatcatacaa gcatgcttgc agcgcacggc gatgcgttcg
     1741 tcgccgaaac cctcggacgc tgccttgggc tgggtcccgg ggacgacgac gatgacgacg
     1801 acccggtgcc gatcggcgac ccgcccgacg acgacgaggg cgacgatcag gacgacgatg
     1861 acgaggacga ggacgacgag gagccgatgc aggtacgccg ttgtgcatcg ccgtatcgcc
     1921 aacgggcgcg tatcacggca caatgtcgcc ccgacgaccg ttcgcgagcc gcggtcccgg
     1981 tcgcccgata cccgatacct gacctctgat ccctgagacc tacctcgcca tcgagcgcgt
     2041 cagcgtcgcc tacgacggcg tgcgcgtgct gtccgaggtc acgctcgacg tcgcgcgcgg
     2101 cgagctcgtc gcgctgctcg gcagctccgg atgcggcaag acgacgctgc tgcgctcgat
     2161 cgccggcttc gtgacgccgc aatcgggccg catccgcatt gcaggacgcg acatcacggg
     2221 gttgccgccc gaaaagcgcg gcaccgcgat gatgttccag tcgtacgcgc tatggccgca
     2281 catgagcgtc gccgggaaca tcggctacgg gatgcgcatg cggggcgctg cacgcggcgc
     2341 aatcgcgcgc cgtgtcgacg agctcctggc gctgatgcag ttgcaagggt atgcagcgcg
     2401 gtcggtcgtg cagctctccg gcggccagcg gcagcgcgta gcgctcgcgc gcgcgctcgc
     2461 cgtcgatccc gcattgctgc tcctcgacga gccgatgtcc aatctcgact acaaggtgcg
     2521 catcgagctg cgccacgagc tgcgcgcgct gcagaagcgc atcggcatca ccgcggtcta
     2581 cgtcacgcac gatcgcgaag aggcgctgac gctcgccgat cgcatcgccg tgctcgacgc
     2641 cggccgcgtc gtccagtacg gctcgcccga ggagattttc catcggccca cgtcggcatt
     2701 cgtcgccggc ttcatgggcg ccgacaatgc gatcgagctg cgccgcgacg agcagggccg
     2761 gctgcgcagc gcggcgggca acggcgacgc gggcgagccc gtgaccgcgc attttcgcag
     2821 cgacgttgcg cgcatcgacg catcggcggc cgcggccggc gacgccgagc tgcgcctgca
     2881 ggggcagatc gtccagacgc tctatgtcgg acaaggttac cggtatcgcg tacgcaccga
     2941 cggcgccgat gtgtgggcgc atgcgagcga tcgcatcaac gaaggcgcgc cggtagccgt
     3001 cgtcgtccca cgcgaggccc tgttgctctt tccgcgcgcc accacccaag gctgatcggg
     3061 gacgcgcggc catcgacgtg atcagcgatt agacctcggg agagatcatg aaagtgcgca
     3121 gtcttttgtc catcgcggcg cttggactcg cgtccatcgc gcccgcggcc gcgcagacga
     3181 cgctcaacgt cgtttcggcc ggcgaccaga acatggtcga ttacgtcaac aactatctgg
     3241 gaccgaaatt cgagcagatg aacccgggcg tcaaggtccg tgcggtcggc accgggcccg
     3301 gcgattccgg ctcgcagaag atctacgaga agctcgaggc gcagcagaag gccggcagcg
     3361 cgacgtggga cgtcgacgtc gccgtggttc accagcgtgg cgcggcgacg atggtcaacg
     3421 gcaagctgct gatgccgtat cgcaacgacg tcgccgccgg caagctcgtg tcgcgcgaca
     3481 cggcgaagaa cgcgctcggc gcgaatgtcg acggctacgt gctgccgatg ttccacagcc
     3541 agatcgcctt cgcgtacaac cccgatctcg tcaaggctcc gccaaagagc ttcgccgagc
     3601 tcgccgagtg ggtaaaaaag aacccgaagc agttcggcta caacggcgtc aagggcggca
     3661 tgtccggcgt cggcttcgtc atgggatggg tggaggcgaa cagcggcatg cgcgacaagc
     3721 tcgaaaaggg cccgtacgac ccgagcgcga aggcgacgat cgacaagtcg ctcgaaagcc
     3781 tcaaggcctt caaccagtac gtcgtgatga ccccggggaa cgccggcacg ctcgacatgc
     3841 tcaatcgcgg ggaaatcgcg atgggcccgg tgtgggtcga catgttctac acgtggatgg
     3901 ccgacggcaa gatgaatccg aaggtgaagc tcgagctgcc cgcacccgga ttgcccgggc
     3961 agccgatgta ttacgtgatc cccgccaagg cggcgaacgc ggcactggcg aagaagttca
     4021 tcgacttcgc ggagagcccc gaagtgcagg ccgacggcat cgtcaagcag ttcaactggt
     4081 atcccggcat cgatccgcaa tacgtgcagt cgaagatgac gccggcggca tggaacaagc
     4141 ttttcaccga catctcgccg tccgacctcg cgagcaaggg acgcccgttc ccgctgtccg
     4201 attacttcac cgacatcgtc gaaggctacg agcgcgtggt gctgaagtag gcgcctcgtc
     4261 gcgctcctga ggcgggacgg cgggcggatg gcggcgatcg ctgaagaagc tctcgtcgaa
     4321 gcgcaacccg gcggcggagg ccgctgggcg gcgctcgcgc tcgtcgcgcc ggcactgctc
     4381 gtcgtcgggc tgttcttcgt cgtgccgctc gtgctttccg ccgtcggcgc gtttcgcacg
     4441 gccggcggcg ggttcacgct cgcccacttc gcgaaggcac tcgatctcta cggcaacgac
     4501 ctgatcttca ccgtcgagat cgtggcattg tcgacgttgc tgattgcgct gatcgccatc
     4561 gccatcgccg gctatctgac gctcggcgag catccgcgcg ccgttgcggt cctgcgctgg
     4621 ctctatcgct ggccgctgtt catcccgttc gtcgtcgcgg gccagatcat gcgcacgttc
     4681 ctcgccaaga acgggatgct gaaccacgtg ctgatcggca ccggcctgat cgagccgatc
     4741 tcggcacaat cgatgctcga ctggcgcgga atcgtcatcg cgttcgtgtg gaagcaggcg
     4801 ccgttcgtga cgctgctgct ggccggtgcg atggcgtcgc tggagcgcca gcacatcgag
     4861 gccgcacgaa acctcggcgc accgcgcctt cgggtcctcg tcgacatcgt gctgccgcag
     4921 gtgcggggca cgctgctggt cgggctcgtg ctctcgttcg tgacgatgct gtcggtgctg
     4981 tcggtgccgc tgatgatcaa tccgaattcg ccgacgatga tcaccgtcga cgtcgcgtac
     5041 cggatcaaca cgttcgccga ttacgcggtc gcgaatgcgc tgtgcctctt gtcgctgctg
     5101 ctcgccgcat tcggcgcggc cttctacctg cgccacgcgg tgcggcgggt ggaggcggga
     5161 tgagcacgat ggccgcacgt ttgctgccga atgcggtgcg ggcgctcgtg ctcgcgctgc
     5221 tcgcattcgc gatcttcggg ccgctcctca atctcgtgct ctgggcgttc gccgagaagt
     5281 ggtatttccc gaacaagctg ccgctggaat acgggttcac cttctggcag cgcgtattct
     5341 cgccgcgcgg caacgcgatc gggtcgctcg gcacgagcat ctggatcgcc gtgctcacgg
     5401 tcgccgtgag cctcgccgtc gccgtccccg ccggttacgc gctcggccgc ctgaagcttc
     5461 cgtggcgcgg cctgatcctg cttgcgttcc tgctgccgca ggcggttccg aatcttcccg
     5521 tctacgtgaa catcgcgcag gtgttctacc ggatcggact cgcgggaacg atcaccggcg
     5581 tcgtgctcgt gcatgcatcg cacgggctcg ttctggcggt gtggatcgcg tcggcggcgt
     5641 tcgcttcggt ggatgtgtcg ctcgaggagg ccgcgcgcaa catgggcgcg tcggcgtgga
     5701 cgtgctttcg cacggtgacg ctgccgcttg ccgcccccgg tctgatggcg agcgccatct
     5761 tcgtgtttct cgaatcggtc gacgagttca ccggcaccta cttcgtcggc gtgcccgacg
     5821 tgacgacgtt gccgctgctc atgttcaatg cgagcatggg cggcaactac cagatcgcgt
     5881 cgatcaccgc gcttttgctg ctcgtgccgt cgatcggctt catgctggtc gtcgagcgct
     5941 tcctgaaggc cgacgtgctc gcgatggtcg gccgctgacg cgtcactcgc ccatctgagc
     6001 gcgcttcttc gacagcgcgc cgatgagccg gtcgagcgca ttcgcatcga gcgccatgcc
     6061 ttcctcgccg aagcgcagcg aaagccgccg cgcctcggtg tcgaagcgca atgtcacgac
     6121 ggcgctgccg ccgagcgaca ccgactcctc gcgcatccgc gtcgacggcg cgacgccctg
     6181 gttgccgatc gtatgcggcg ccttgcgcgg cgcttcatgt ttcggatcgc gttgttccgc
     6241 gccgggttcg ggcgcgcccg ccgctcggcc cggcaactgg gtggcgtcgc tcgccggaaa
     6301 ggagtcctcc accatccggt cgatcgtctg ttcctggcgc gtctcgcctt tcggcggccg
     6361 cgtgtgcctc ggttgcgtca tggtcatcct cgtgagtcgg cgcggcaagc tcgcgctcga
     6421 tcgttcgaca gaagcgtgcc ggcgccgttg cgtcgctgcg gccatccgat gaggctagca
     6481 gtggccatgc catcggcaac aggccgcgtg gcagccgcat cggcacggtc gacgttgcgc
     6541 ctcgggaatc cccggccacg taagttctgc cggatcaagt taccggactc ctccgttgtg
     6601 cactgcacaa tcggcccccg ggtcgtgcta caatcgtgtt cgttgcggtg ctctgcgaag
     6661 tcgtcggttg cccgccgcaa cctgggaatt tctccaaggc atccgatcat cgacttctaa
     6721 tccgaggacc ggttcaggtc ctgcgccctg tggcgtttca gcgggcatcg tccctcgatg
     6781 ccaatgctcc cggttagcga cgatggcttt gcagtgacgt cgacccggta gcggctgtac
     6841 tcgcgatccc gacccctggc tttcgctccg gcgaaagcgg ggtgtcggcg cgccctcatc
     6901 tgcgatagcc ggactgctgc gcgcgccatg gactgcgcgc gcggccactt gccgtccgtt
     6961 cgccttcgcg agcaggacgg cgttgtccgc cgattggaaa aacgagaatg cgattcgaag
     7021 aactgaaatt gatgcagccg ctgttgcagg cgcttgccga atgtggctat gacgagccga
     7081 cgcccatcca ggcacagacc atcccggtgc ttctcgccgg ccgcgacgtg atcgcgtcgg
     7141 cggaaaccgg caccggcaag acggccgcct tcatcctgcc cgcgctgcag cggctgtcgg
     7201 agcagggtgt gcgacccgcg ccgaagcgtc gcggtgccgg cgtgccgaga gtactcgtgc
     7261 tcgcaccgac gcgcgagctc gcgcagcagg tcgcgacgca gtcgtcgcgc tacgggcaga
     7321 acctgcgcct tcgcaccg
//
LOCUS       DASSVX010000021         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1400632, whole genome shotgun sequence.
ACCESSION   DASSVX010000021 DASSVX010000000
VERSION     DASSVX010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400632"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VFO53_01045"
     CDS             <1..652
                     /locus_tag="VFO53_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET8874887.1"
                     /translation="NSVVLADEINRAPPKTQSALLEAMEERQVSQDGQTRVLPDPFFV
                     IATQNPVEQIGAYPLPESQLDRFLLAIELGYPDAVAERELLAGGDRRTKLVDVVPQCD
                     AATLVDWQREAAEIHVAPPLLDYVQSLLAASRGHLPGQTAGASGEAGARRGLSPRAGL
                     MLLSAARAWALMSGRPMAIPEDVQAVFPSVAGHRLAGGARAGALQAQQLIRAVALP"
     gene            715..1650
                     /locus_tag="VFO53_01050"
     CDS             715..1650
                     /locus_tag="VFO53_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET8874888.1"
                     /translation="MRQRAFRRDPTDLLPVVLRHSRIYILPTRRGYAVIATLVTMLLT
                     SLNYALALGLGVTFLLSGLVAAALLHTFRNLAGIAIKPLAASDAFAGGHVVFSLAVSA
                     GAIDRHAITIGANNAAAKTAIATGSTQTVTLAAAAVVRGRIPLGRITLSSRYPLGLWR
                     GWAYVHFPLSGIVYPAPEAHPPPLPTGAQGIDPHMTGRGDDADLAGLREYQRGDPLQR
                     VAWKAVARGAGWFSKSFEGTSGGGPIALDFARLPAQLDAEKRLSRLCAWVLAAERTTR
                     PFALTVPGESLPTGSGREHRRAALTVLALVPADTQ"
     gene            1647..>3011
                     /locus_tag="VFO53_01055"
     CDS             1647..>3011
                     /locus_tag="VFO53_01055"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HET8874889.1"
                     /translation="MTAAASLLGRLKRSATRSSQRASTDPALTPAQIQSLGILLVAAQ
                     LPQAVHLPIWVALFGIALVALRFVLLRRDRLRPAATPARIPSWALAVFALAVAFAIRK
                     SYGYFVGRDPCVAFLFVLVGIKFLEVRTLRDGTLLVCLAIFLLITPFFYSQSLIAALA
                     ALPAVLAAGAAMESLAHDDPLATPTAWRVAWRRTAAMLAQGLPIAAALFLLFPRLATP
                     LWGLPADRAAATGLSERMAPGSISELALNDAVAFRVDFDSAPPPPRARYWRGPVLALF
                     NGREWRSLSPSPGGRLAVVDRPTIRYTVTLEPNDHPWLFALDFPSAMPQIDSDAGTQT
                     SAGGFAGYSREHQLMMRAPVTQALRYSVQSTLTDHYPVPSARDAQINSRPPPGNPRTA
                     QFARELRAANPDDRRFASAILQWFHDEQFFYTLAPPLLEEDPVDAFLFDLRRGFCEHY
                     ASAFV"
BASE COUNT          415 a         1030 c         1003 g          563 t
ORIGIN      
        1 caactcggtc gtgctcgccg acgaaatcaa ccgcgccccg cccaagacgc agagcgcact
       61 gctcgaagcg atggaagagc gacaggtgtc gcaggatggt cagacgcggg tgctgcccga
      121 tccgtttttc gtcattgcga cgcagaaccc ggtcgagcag attggcgcat atccactgcc
      181 cgagtcgcaa ctcgaccgct tcctgctcgc catcgagttg ggctatcccg atgccgtcgc
      241 cgagcgtgag cttctggccg gaggcgatcg gcgcacgaag ctcgtggatg tcgttccgca
      301 atgcgatgcg gcgacgctcg tcgattggca gcgcgaggct gccgagatcc atgtcgcgcc
      361 gccgcttctc gattacgtgc agtcgctgct cgcggcttcg cgcggccatc tgcccgggca
      421 gacggcgggt gcgagcggcg aagcaggcgc acgccgcggc ttgagcccgc gcgcggggct
      481 gatgctgctc tccgcagcac gtgcgtgggc gttgatgagt ggacgtccga tggcgattcc
      541 cgaggacgtg caggccgtgt ttccgtcggt ggccggccat cgtctcgccg gcggcgcgcg
      601 cgccggcgca ttgcaggcgc aacaactgat tcgcgccgtc gcactgccgt gattgccgtc
      661 accgcaccac ccgtgcggac gctccgcgcg gcgctggcgc acggatggtc acggatgcgg
      721 cagcgggcct tccgccgcga tccgacggat cttctgccgg tggtgctgcg gcattcgcgg
      781 atctacatcc ttcctacacg ccgcggttat gcggtgatcg cgacgctcgt cacgatgctg
      841 ctgacgtcgc tcaattacgc gctcgcactc ggcctcggcg tgacgttcct gttaagtggc
      901 ctcgtcgccg ccgcgctgct ccacacgttt cgcaacctgg cgggcatcgc gatcaagcca
      961 ctcgcagcga gcgatgcatt cgcaggtggt cacgtcgtgt tctcgcttgc tgtgtccgcg
     1021 ggcgcgatcg atcgccacgc gatcacgatc ggcgccaaca acgcggctgc aaaaacggcg
     1081 atcgcgaccg gatcgacgca aacggtgacg cttgccgccg cggccgtcgt gcgcgggcgc
     1141 attccgcttg ggcggatcac gctgtcctcg cgctatccgc tcggcttgtg gcgcggctgg
     1201 gcctacgtgc attttccgtt gtccggcatc gtctatccgg cgcccgaggc gcatccgccg
     1261 ccgctgccaa ccggcgcaca ggggatcgat ccgcacatga ccggtcgcgg cgacgatgcc
     1321 gatctcgccg ggctccgcga atatcagcgc ggtgatccgc tgcagcgtgt cgcgtggaaa
     1381 gcggttgcgc gcggcgccgg ctggttctcg aaatcgttcg agggcaccag cggcggcgga
     1441 ccgatcgcgc tcgacttcgc gcgattgccg gcgcagctcg acgccgagaa gcgactgtcg
     1501 cggctctgcg catgggtgct tgcggccgag cgaacgacgc ggccattcgc gttgaccgta
     1561 ccgggtgaat cgctgcccac gggatcgggc cgcgagcacc gtcgcgccgc gctgacggtg
     1621 cttgcactcg ttccggcaga cacgcaatga cagcggcagc ttcgctgctt gggcggctca
     1681 agcgatcggc gacacgctcg tcgcagcgag cgtcgacgga cccggcgctg acgcccgcgc
     1741 agattcagag cctcggcatt ctgctcgtcg cggcgcaatt gccgcaggcg gtgcacctgc
     1801 cgatctgggt cgcgctgttc ggcatcgcgc tcgtcgcact gcgcttcgtg ctgctgcggc
     1861 gcgatcgctt gcgtcctgca gccacgccag cgcgaatccc gtcgtgggcg ctcgcggtgt
     1921 tcgcgctcgc ggtcgcgttt gcgattcgca agtcgtatgg atatttcgtc ggccgcgacc
     1981 cgtgcgtcgc cttcctgttc gtgctcgtcg gcatcaagtt tctcgaagtg cgcacgctgc
     2041 gtgacggaac gctgctcgtg tgcctcgcga tcttcctgct gatcacgccg ttcttctaca
     2101 gccaatcgct catcgctgcg ctcgcggcgc tgccggccgt gctcgcagcc ggtgccgcga
     2161 tggagtcgct ggcgcacgac gatccactgg caacaccgac cgcatggcga gtggcgtggc
     2221 gtcgcacggc cgcaatgctc gcgcagggtc tgccgattgc tgcggcgctg ttcctgttgt
     2281 tcccgcggct tgcaaccccg ttgtggggat tgcccgccga ccgcgcggct gcgacggggt
     2341 tgtcggaacg gatggcgcca ggttcgatca gcgagctcgc gttgaatgac gccgtcgcgt
     2401 ttcgcgtcga cttcgacagc gcgccgccgc caccgcgtgc gcgttactgg cgcggaccgg
     2461 tgctcgcgct tttcaatgga cgcgaatggc gatcgctgtc gccttcgcca ggcggtcggc
     2521 tcgccgttgt cgatcgtccg acgattcgtt acacggtaac gctcgagccc aacgatcacc
     2581 cgtggttgtt cgcgctcgac ttcccctcgg cgatgccgca gatcgattcc gatgccggca
     2641 cgcagacgag cgccgggggg ttcgctggtt attcgcgcga gcaccagttg atgatgcgag
     2701 cgccggtgac acaagcgctg cgctattccg tgcagtcgac gctcaccgat cactatccgg
     2761 tgccgtcggc gcgtgacgcg cagatcaatt cgcggccgcc acccggcaac ccgcgcaccg
     2821 cgcagttcgc acgcgaattg cgcgccgcga atcccgatga tcggcgattc gccagcgcga
     2881 tcctgcaatg gtttcacgac gagcaatttt tctacacgct cgcgccgccg ctgctcgagg
     2941 aggatccggt cgatgccttc ctgttcgatt tgcggcgcgg tttctgcgag cactacgcaa
     3001 gcgcattcgt c
//
LOCUS       DASSVX010000022         4667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_234113, whole genome shotgun sequence.
ACCESSION   DASSVX010000022 DASSVX010000000
VERSION     DASSVX010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234113"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..1240
                     /locus_tag="VFO53_01060"
     CDS             485..1240
                     /locus_tag="VFO53_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET8874890.1"
                     /translation="MPVAAWADDGDGSPCAALPSHAALKSALIAAVQTETSGLDFQMW
                     GTIVNRDGIVCAVAFSGANRGSQWPGSRAISAQKANTANAFGLDGLALSTANLYSATQ
                     PGGSLFGLQHSNPVDTDVAYGGSPSAYGTAHDPMVGRRIGGVNVFGGGLGLYNAQHRI
                     VGGVGVSGDTSCADHMIAWRARANLGLDHLGTVVGGPAKLFASDPTHPDNIIFDITPN
                     PDGGTGKSATGFGHPKCGINDTTDPLSLPPVSP"
     gene            complement(1295..2599)
                     /locus_tag="VFO53_01065"
     CDS             complement(1295..2599)
                     /locus_tag="VFO53_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET8874891.1"
                     /translation="MPTNKRPEPTIVRPDDIDPHHRFDRPLAAPGHTQVDFEQRVDFR
                     RLHDYRLARTRAALAHSDLGALLCFDQHNIRYITSTVIGEWARDKLTRYSLLTGNGDP
                     YLWDFGSAARHHRLHAPWLHHDHCRAGLLGLRGATGGDLTLFRDAAKEIRAILDAEGV
                     ADMPLGLDVVEPPMLFELQKLGIEVRDGQRTMLRAREVKNVDELTLLNMAAAMVDGVY
                     HDIAEALRPGIRENDIVALATKRLYEMGSDNVEAINAISGERCSPHPHNFTDRLIRPG
                     DQAFFDVIQSFMGYRTCYYRTFSVGRATPAQRTAYGKAREWMDRTIALLKPGVTTDTI
                     AKSLPIAGDIGFASEMDAFGLNFCHGLGLGLHERPLISRLNSLKEPIELKAGMVFAVE
                     TYCPASDGVSAARIEEEVILTPGGPQVISLYPAEELPIANPY"
     gene            complement(2608..3615)
                     /locus_tag="VFO53_01070"
     CDS             complement(2608..3615)
                     /locus_tag="VFO53_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8874892.1"
                     /translation="MRAIAAVVAGIALALMGVTAAAQGQAPALTPLRVLAFDGGWNLP
                     IWAGQRNGFFEAQGLRVELAYTPSSGFLVKSVLDGKSDVAFAAIDNVIAYQEGQGEAS
                     VPDNPDLFAFMGGDGGFISIVASPAVKSVTDLKGRTVSVDAMTTGFAFVVRELVARNG
                     LAESDVNFVRAGGTANRYQDLVAGKHDATLLRTPFELLAENRGYHRIASAETLGAYQG
                     TVGLARRSWAREHEAALIGFLRGYKAATDWLYDPANREIAEALLVAHIRDMTPALAKR
                     SYELLVSDKGGLSRDLAPDVAGIATVLQLRGKYATPRKALSDPMKYVDLGYYNKAFGK
                     Q"
     gene            complement(3640..4494)
                     /locus_tag="VFO53_01075"
     CDS             complement(3640..4494)
                     /locus_tag="VFO53_01075"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HET8874893.1"
                     /translation="MQIERVGVIGAGTMGNGIAQACATAGVAVVMVDVAQAAVDRGIA
                     AIGASLERLQKKDRITGDARAAALARVRGSIAYGDLAGANLIVEAATENVELKLSILR
                     KLDGMAAPDTILASNTSSISITALAAVVSRPERFLGMHFFNPVPMMALVELIRGLQTS
                     DDTLRIVRAFAERLGKAPIVVKNSPGFVVNRILCPMINESIFALQEGLASAEDIDNGM
                     RLGCNHPIGPLALADMIGLDVMLAVMNVFYADFNDPKYRPAPLLKEMVAAGYLGRKTK
                     RGFYRYEG"
BASE COUNT          750 a         1571 c         1574 g          772 t
ORIGIN      
        1 cgaggtcctt ctcgcgcgtc gccggatgat cgggacgcac gtgcgcatgg tgcagcatga
       61 gccgcggaaa ggtatcgatc ttttcgtcca ttcgccgcta ctcctcgcgt cgcgcgtatg
      121 ttacctgcgc gcgacccacg cggcgaactt ccgcccggtc gggattggtg cggtagcggt
      181 cgaccgatgc gcggttcgcc gggatagccc aggttattcg ccgataacac cgccggtccg
      241 cgatgtcgac cgggcttaca cccgtcgctc gcgcccctgc gtcgtagcaa cgcgaaagga
      301 gccgaatcat cctgttgtag acgacggctt gttcattgct tgcgtcgaat cgtccttcgc
      361 ccgacatcgc acgggcgccc ggtgtgtcga cagacggtgc cggaacgcga ggaccatcga
      421 cgacaaggag acgatcatgc aacgacatgt ccgttcgtgc gttttgattt tgggattggc
      481 ggcaatgccg gtcgccgcat gggccgatga cggcgacggg tcaccgtgcg ccgcactgcc
      541 gagccatgcg gcgctgaaga gcgcgctgat cgcggcggtg cagaccgaga ccagcggcct
      601 cgacttccag atgtggggaa cgatcgtcaa tcgcgatggc atcgtctgtg ccgtcgcatt
      661 ctcgggcgcg aaccggggat cgcaatggcc gggtagccgg gcgatctcgg cgcagaaagc
      721 gaacacggcc aacgcgttcg gcctcgacgg cctcgcgctc tcgaccgcga acctctactc
      781 ggcgacgcag ccgggcggat cgctcttcgg attgcagcac agcaatccgg tcgataccga
      841 cgtcgcgtac ggcggcagtc cttccgccta cggcaccgcg cacgatccga tggtcgggcg
      901 gcgcatcggc ggcgtcaacg tgttcggcgg cggcctcggc ctgtacaacg cgcagcacag
      961 gatcgtcggc ggcgtggggg tcagcggcga cacgtcgtgc gccgaccaca tgatcgcgtg
     1021 gcgcgcgcgg gcgaacctcg gcctcgatca tctcggcacg gtcgtcgggg gaccggcgaa
     1081 gcttttcgcc agcgacccga cgcacccgga caacatcatc ttcgacatca cgccgaatcc
     1141 cgatggtggc acagggaaga gcgccacggg cttcgggcac ccgaaatgtg gcatcaacga
     1201 cacgaccgac ccgctgtcgc tgccaccggt cagcccgtag ccggctttgc gtgggcgcga
     1261 caccgacacc tcgcgcccac gcgaaatcag aaagtcagta cggattggcg atcggcagct
     1321 cctcggccgg ataaagcgag atgacctgcg gcccgccggg cgtgagaatc acctcttcct
     1381 cgatgcgcgc cgccgacacg ccgtcgctgg ccgggcagta ggtctcgacg gcgaacacca
     1441 tccccgcctt caactcgatc ggctccttca gcgagttgag ccgcgagatc agcggccgct
     1501 cgtgcaggcc gaggccgagg ccgtggcaga aattcagccc gaacgcgtcc atttcgctcg
     1561 cgaagccgat gtcgcccgcg atgggcagcg acttcgcgat cgtgtccgtc gtgacacccg
     1621 gcttcagcaa cgcgatcgtg cggtccatcc actcgcgcgc cttgccgtac gcggtgcgct
     1681 gcgcgggcgt cgcgcggccg acgctgaacg tgcggtagta gcaggtgcga tagcccatga
     1741 acgactggat cacgtcgaag aatgcctggt cgccggggcg gatcagccgg tcggtgaagt
     1801 tgtgcgggtg cggcgagcaa cgctcaccgg aaatggcgtt gatcgcctcg acgttgtcgg
     1861 agcccatttc gtagagccgc ttggttgcga gcgcgacgat atcgttctcg cggatgcccg
     1921 gcctcagcgc ttcggcgatg tcgtggtaca cgccgtcgac catcgcggcg gccatgttga
     1981 gcagcgtcag ctcgtcgacg ttcttcactt cgcgtgcccg cagcatcgtc cgctgcccgt
     2041 cgcgcacctc gattccgagc ttctgcagct cgaaaagcat cggcggctcg acgacgtcga
     2101 gcccgagcgg catgtcggcc acgccctcgg catcgaggat cgccctgatc tccttcgccg
     2161 cgtcgcgaaa cagcgtgagg tcgccaccgg tggcgccgcg caagccgagc agacccgcgc
     2221 ggcaatgatc gtggtgaagc cacggcgcgt gcagccggtg atgccgcgcg gcggagccga
     2281 aatcccacag gtacgggtcg ccgttgccgg tcaggagcga atagcgcgtc agcttgtcgc
     2341 gcgcccattc gccaatcacc gtgctcgtga tgtagcggat gttgtgctgg tcgaagcaca
     2401 ggagcgcgcc gaggtccgag tgcgcgaggg ccgcgcgcgt gcgtgcgagc cggtagtcgt
     2461 gcagacgccg gaaatcgacg cgctgctcga agtcgacctg cgtatgcccg ggtgcggcca
     2521 gcggccggtc gaagcgatgg tgcgggtcga tgtcgtcggg ccgcacgatc gtcggctcgg
     2581 gccgtttgtt cgtcggcatg acgcgcgcta ctgcttcccg aacgccttgt tgtaatagcc
     2641 gagatcgacg tatttcatcg ggtcggaaag cgccttgcgc ggcgtcgcgt atttgccgcg
     2701 cagttgcagc accgtggcga tgcccgcaac gtcgggggcg agatcgcgcg acagcccgcc
     2761 cttgtccgac acgagcagct cgtacgatcg cttcgcaagc gcgggcgtca tgtcgcggat
     2821 gtgtgcgacc agcagcgcct cggcgatctc gcgattcgcg ggatcgtaga gccagtcggt
     2881 cgccgccttg tagccgcgaa gaaacccgat cagcgccgcc tcgtgctcgc gcgcccagct
     2941 gcgtcgcgcg agtcccaccg tgccctggta cgcgcccagc gtctcggcgc tcgcgatgcg
     3001 gtgatagccg cgattctcgg cgagcagctc gaacggcgtg cgcaacagcg tcgcgtcgtg
     3061 cttgcccgcg acgagatcct ggtagcggtt ggcggtgccg ccggcgcgca cgaagttcac
     3121 gtcggattcg gcgaggccgt tgcgcgcgac gagctcgcgc acaacgaacg caaagccggt
     3181 cgtcatcgcg tcgaccgaca ccgtccggcc cttgagatcg gttacgctct tcaccgccgg
     3241 cgaggcgacg atcgagatga agccgccgtc gccgcccatg aacgcgaaaa ggtcgggatt
     3301 gtcggggacc gacgcttcgc cctgcccttc ctggtacgcg atcacgttgt cgatcgccgc
     3361 gaacgcgacg tcggacttgc catcgagcac cgacttcacg aggaagcccg agcttggcgt
     3421 gtaggcaagc tccacgcgca gtccctgcgc ctcgaagaag ccattgcgct gcccggccca
     3481 gatcggcagg ttccagcctc cgtcgaacgc gagcacgcgc agcggcgtga gcgcgggcgc
     3541 ctgcccctgc gcggcggcgg tgacgcccat caatgcgagc gcgatgcccg cgacgactgc
     3601 tgcgattgcg cgcatggcaa ccacctctcc gctgacccgt cagccctcgt aacggtagaa
     3661 gccgcgcttc gtcttgcggc ccaggtaccc ggcggcgacc atttccttca ggagcggcgc
     3721 cggacgatac ttcggatcgt tgaagtcggc atagaagacg ttcatcacgg ccagcatcac
     3781 gtcgaggccg atcatgtcgg cgagcgccag cggcccgatc ggatggttgc agccgagccg
     3841 catgccgttg tcgatgtcct cggccgacgc gagcccttcc tgcaacgcga agatcgactc
     3901 gttgatcatc gggcagagga tgcgattcac gacgaatccg gggctgttct tcaccacgat
     3961 cggcgccttg ccgaggcgct cggcaaacgc gcgcacgatg cgcagcgtat cgtccgacgt
     4021 ctgcagcccg cgaatcagct cgaccagcgc catcatcggc accggattga agaagtgcat
     4081 gccgaggaag cgctccggcc ggctgacgac cgccgcgagc gcggtgatcg agatcgacga
     4141 cgtgttcgac gcgagaatgg tatcgggagc ggccatgccg tcgagcttgc gcaggatgga
     4201 cagcttgagc tcgacgtttt cggtggccgc ctcgacgatc aggttggcgc cggcgagatc
     4261 gccgtaggcg atgcttccgc gcacgcgcgc cagcgccgcg gcacgcgcgt ccccggttat
     4321 cctgtccttc ttctgcaggc gctcgaggct ggccccgatc gctgcgatgc cgcgatcgac
     4381 ggcggcttgc gcgacgtcga ccatgaccac ggcaacacct gccgtcgcac aggcctgcgc
     4441 gatgccgttg cccatggtac cggcgccgat gacgccgacg cgctcgatct gcatcgcgat
     4501 tcccgactgg cgaatggacg ttcgcgcatt atgccttgcc gacgcgacct ttcgcgcgcg
     4561 cggccagcga tcgtttgccg gggggcggcc gggttttgcg ggcacgccgg ggcggcgccg
     4621 cgcgaagccc cgcgagcatc gtgtcgacca gcgcgggtgc ggcggcc
//
LOCUS       DASSVX010000023        12846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_767388, whole genome shotgun sequence.
ACCESSION   DASSVX010000023 DASSVX010000000
VERSION     DASSVX010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12846
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767388"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFO53_01080"
     CDS             <1..197
                     /locus_tag="VFO53_01080"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET8874894.1"
                     /translation="LTNLIQFDAAANPGNSGGPLVTMDGAVVGIVTGILNPNNQRVFI
                     GIGFAVPIENAAAAVGLSPF"
     gene            223..1236
                     /locus_tag="VFO53_01085"
     CDS             223..1236
                     /locus_tag="VFO53_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET8874895.1"
                     /translation="MENRENGGSDVASLMQRILYEVKKVVVGQDHFLERVLVAMLAQG
                     HLLVEGVPGLAKTLTVKTLAKVIRGSFKRIQFTPDLVPADLVGTRIYNQKTGDFTTSL
                     GPVFTNLLLADEINRAPAKVQSALLEVMQEYQVTIAGQTHMVPTPFLVMATQNPIETE
                     GTYPLPEAQVDRFMMKVLVGYPSEEEEFVIVERVTGLAADVSTVASTDQLAALQRQCR
                     SGYVDPSLIQYAVRLAGATRFPDRYGVGDFAKYVTFGASPRASISLIEGARALAYLRG
                     RDYVLPDDVSDLAPDVLRHRLVLSYEALADGLTPDDLVHKVMQRIVPPEKPLESHVKF
                     ARA"
     gene            1298..2263
                     /locus_tag="VFO53_01090"
     CDS             1298..2263
                     /locus_tag="VFO53_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET8874896.1"
                     /translation="MTNVVPLDSARSAERALRRLEWTVLRRLDGLLHGDYRTLFRGFG
                     LDLADLREYQYHDDVRHIDWNVTARLATPYVREYNEDRDVTAWFLLDLSPSVDFGSGD
                     VRKRNVSTDFVTVLARILTRHGNRVGAAFYGETMDTVVPAGSGRRHVLQILHRMLHRP
                     APARASATNLHEFLRAAFPVIARRSLVFVVSDFISAASWAAALAQFGERHEVIAVRLY
                     DPLEHELPDLGLLTMQDAETGEQVFVDTHDRGFRRRFVKAAEAREASLRAGFRDAGVD
                     VLELSTSGDLADAILRFADLRKRRSRLAAGAVMPRGEQQHVVPLA"
     gene            2244..3293
                     /locus_tag="VFO53_01095"
     CDS             2244..3293
                     /locus_tag="VFO53_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET8874897.1"
                     /translation="MSFLWPDFLWLLALVPALVGLYLLALRRKKRLAVRYASLSMVRD
                     ALAMGQRVRRHVPPALFLVGLALMIVAIARPTATVKLPTQHELVILAMDVSGSMRAVD
                     VKPTRLAAAQEAARAFINDQPSNVRIGIVAFAGTAAVVQAPTENRDELLSAIDRFELQ
                     RATAIGSGLLVSLKAIFPDVEFDLRASNPRPHASDRHGMAIDQPRDAAKEPPKPVAPG
                     SYKSAVIILLTDGQTTTGPDPIESAKIAAERGVRVYTVGVGTVQGEVIGWEGWSMRVR
                     LDEDSLKQIANVTRGEYFYAGTATDLKNIYKGMSSKMELTKQQTEVTALFVAAAALFV
                     LFGAGLSLVWFNRLL"
     gene            3326..3787
                     /locus_tag="VFO53_01100"
     CDS             3326..3787
                     /locus_tag="VFO53_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017099480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET8874898.1"
                     /translation="MSRHTAQVSWTRGDARFTDGRYSRGHVWRFDGGVEVPASASPHA
                     VPPPWSRAEAVDPEEAFVASISSCHMLWFLSIAAKRDYTVEEYVDDAEGFLEQDAEGR
                     MSMTRVVLRPRIAFAAARPASSEAIQAMHDEAHHACYIANSVRTAISVEAR"
     gene            complement(3796..4899)
                     /gene="rodA"
                     /locus_tag="VFO53_01105"
     CDS             complement(3796..4899)
                     /gene="rodA"
                     /locus_tag="VFO53_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HET8874899.1"
                     /translation="MVQLIGRVWEVLTRRIDSMLFAAALAIVGVGLVTLFSAADQSVA
                     RETSQAMSLSFAIVLMWMFANIAPQTLARAAVPLYVVAVAMLIGVATFGVVVNGSRRW
                     LNLGVARFQPSELMKVALPLMLAWYFQKFEGRIGWRDFVFAAILIVVPVYLIKRQPDL
                     GTALLIGASGFYVLFLAGLSWKVIVGLVGLGAAVGPMVWTHLHDYQRERILTFLDPSR
                     DPLGAGYHSTQASIALGSGGIVGKGWLNGTQTHLDFLPERHTDFIFAVFGEEFGLIGN
                     ALLLILYLLLIGRAMMITAHASTLFARLLSGAITLMFFTYAFVNMAMVSGILPVVGVP
                     LPLVSYGGTALISLFIGLGILMSVQAHRKLVTT"
     gene            complement(4922..6883)
                     /gene="mrdA"
                     /locus_tag="VFO53_01110"
     CDS             complement(4922..6883)
                     /gene="mrdA"
                     /locus_tag="VFO53_01110"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET8874900.1"
                     /translation="MSRRLLFIRRDSGGFGTSELRNPERDLYHFRRRLSLVAALVFIA
                     FCGLFARFFYLQVVQHAHFETLAETNRIAIVPIVPNRGVITDRNGIVLAQSYSAYTLE
                     ITPSRVANLDETIDKLSQIVDVEPRDRKRFRKLLEESRTFESLPIRTKLTDEEVARFA
                     VNRYRFPGVDIKARLFRQYPFGEIASHVIGYIGRINDKDNERIAAWDETANYKGSDYI
                     GKVGVELSYERELHGTTGVEDVEVDAGGRAVRTLRRTPPTAGNNLTLSLDIRLQQVAE
                     AAFGDRRGALVAIDPTSGDVLAFVSKPGYDPNLFVDGIDPANWQELNDSPDKPLLNRP
                     LRGAYPPGSTIKPYLALAALTSGKRTPAQTIYDPGFFQIPGQAHRFRDDKPGGHGTVD
                     MYKSIVQSCDTYYYMLANDTDIDDTARFLGKLGFGQKTGIDVEGELTGVLPSREWKRQ
                     RFAGKNYREEHRKWYLGDSISAGIGQGYNAFTPMQLAHAIAMIANDGVGYTPHLVKSI
                     TNVKTGATREIASQPSSRLDVRPADLAMIKRALVGVNKEGTSAAAFKDAKYVSAGKTG
                     TAQVYSLKGEKYSEHHVDERLRDHAWFEAYAPADHPTIAVAVLVENGGFGASAAAPIA
                     RKVFDRYLLGESEPGAAKDMPGAGDDEGD"
     gene            complement(6906..7442)
                     /gene="mreD"
                     /locus_tag="VFO53_01115"
     CDS             complement(6906..7442)
                     /gene="mreD"
                     /locus_tag="VFO53_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET8874901.1"
                     /translation="MRVLPHVGSFGPAAPEEILRPARPWFVLLSIFVALLLNLVPLND
                     PVMTYRPDFVALVLLYWCIQEPRYVGVGSAWLVGLLMDVGDATLFGQHALAYALLAYG
                     AEFFRRRVLRFPLWQQAVQVAVLLVLCALLVLLVRFVGGAPLPKWTYVAPPLVGALLW
                     PLVSVLLQTPQRPSRSRR"
     gene            complement(7470..8387)
                     /gene="mreC"
                     /locus_tag="VFO53_01120"
     CDS             complement(7470..8387)
                     /gene="mreC"
                     /locus_tag="VFO53_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET8874902.1"
                     /translation="MRPIPVDTPAFFNRGPSPLARLAFFGLLSLALLFADTRYRYLES
                     VRQAVAIVLYPVERALEMPGAAFAYVSDYFGSKRRLADENAMLRQELVARAPATQGYT
                     REQEENARLRALLDVRAQYAATAVAVQVLYTSRDPFTQKVFVDKGASAGVAAGAGVID
                     ASGVVGQVTRVFPYMAEVTLITDRDQAVPVQVERSGARSVLFGNGTGRSPELRFTSPS
                     ADIRAGDRLVTSGLDHTYPPGLAVAEIVDVVRDSGQMFARILCKPLAGIDHSEFLLVL
                     SQSASMPARPEEPGDAEAAKKGGRVRGRR"
     gene            complement(8492..9535)
                     /locus_tag="VFO53_01125"
     CDS             complement(8492..9535)
                     /locus_tag="VFO53_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET8874903.1"
                     /translation="MFDSFKGYFSSDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRQE
                     GGPNGKKVIQEVGLAAKQMLGRTPGNITAIRPMKDGVIADFTVTEQMLKQFIKKVLDS
                     RLFSPSPRIIICVPCGSTQVERRAIRESALGAGASKVFLIEEPMAAAIGADLPVGDAT
                     GSMVVDIGGGTTEVGVISLGGMVYAGSVRVGGDKFDEAVVNYIRRNYGMLIGETTAEI
                     IKKTIGSAFPGSEVKEMEVKGRNLAEGIPRSFTVSSNEILEALTDPLNSIVSAVKSAL
                     ERTPPELGADIAERGMVLTGGGALLRDVDRLLMEETGLPVVVAEDPLTCVVRGCGKAL
                     ENMEKLQTIFTSD"
     gene            9697..9984
                     /gene="gatC"
                     /locus_tag="VFO53_01130"
     CDS             9697..9984
                     /gene="gatC"
                     /locus_tag="VFO53_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET8874904.1"
                     /translation="MSFSLDDVARLARLARIDLDADTVRDVRDKLDAIFALIDALQSI
                     DTAGVAPMAHAQDIMLPLRDDAVTAGDCRELYQAQAPAVAGGLYLVPKVIE"
     gene            9981..11492
                     /gene="gatA"
                     /locus_tag="VFO53_01135"
     CDS             9981..11492
                     /gene="gatA"
                     /locus_tag="VFO53_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET8874905.1"
                     /translation="MTAAPAPGAYRAATIGELARALRGRELSSVELAQQTLSRIGGSQ
                     AALNAFITVDADRALVEARRADAALARGDASPLTGIPIAHKDVLMTAGLRTTCASRML
                     ANFVAPYDAHVVARLREAGTVLVGKTNMDEFAMGSSSETSHFGPVRNPWSAACVPGGS
                     SGGSAAAVAAGLLPATTGTDTGGSIRQPAALTGVSGLKPTYGVCSRYGLIAFASSLDT
                     PGPFARTAEDCALLLNAMAGHDERDCTSLDRPRENYLRALHAPATARPLAGVRIGLPR
                     EYFTDGLEREVASAVDAALGELRALGATTVDIDLPMVRHAVAVYYVIAPAEASSNLSR
                     FDGVRYGHRAARYSDLTDMYCRTRAEGFGAEVKRRILVGTYVLSHGYYDAYYLKAQQV
                     RRLIAQDFLRAFGECDVIAGPTAPTCAFAIGAKADDPVQMYLNDIFTIAANLTGMPAI
                     SIPCGFTRDALPIGLQLQGNHFDEARLLNVAHRFQQATDWHLRCPSPQPSPDA"
     gene            11489..>12846
                     /gene="gatB"
                     /locus_tag="VFO53_01140"
     CDS             11489..>12846
                     /gene="gatB"
                     /locus_tag="VFO53_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET8874906.1"
                     /translation="MNWEIVIGIETHAQLSTVSKIFSGAATAFGAAPNTHASAVDIAL
                     PGVLPVPNRGAVDRAIRFGLAVGGTINRRSVFARKNYFYPDLPKGYQISQYEIPVVQG
                     GTVTIATGAGERQVRLTRAHLEEDAGKSLHEGLPRELGDVTGIDLNRAGTPLLEIVSE
                     PDMRSAEEAVAYAKALHSLVTWIGICDGDMQEGNFRCDANVSVRRTGEPLGTRCEIKN
                     LNSFRFLAQAIEHEARRQIELIEDGGRVVQQTRLYDPDRNETRPMRSKEDAQDYRYFP
                     DPDLPPLTIDDAWIERARAALPELPAAMRARLVAQHGITDYDAAQLTASRDLVDYYNA
                     VCAALPAADAASHKHVANFVLGEFAAARNRDDIAVHASPVAPEQLAELLRRLQDDTLS
                     RMKAKDVFDEMWNRSRNVSVQLTGAEMRATSGMVDAIIEQRGLRQISDAAAIETLVEQ
                     VIA"
BASE COUNT         2147 a         4375 c         4280 g         2044 t
ORIGIN      
        1 tgctcaccaa cctgatccag ttcgacgcgg cggccaatcc gggcaattcc ggcgggccgc
       61 tcgtgacgat ggacggcgcc gtcgtcggca ttgtcaccgg catcctcaat ccgaacaatc
      121 agcgagtatt catcggcatc ggctttgcgg tgccgatcga gaacgccgcg gcagcggtgg
      181 gcctctcccc cttctaatcc gcaggcgttg caaaaggagc gtatggaaaa ccgggaaaac
      241 ggcggcagcg acgttgcttc gctgatgcag cgcattctgt acgaagtgaa gaaggtggtc
      301 gtgggccagg accacttcct cgagcgcgtg ctggtcgcga tgctcgccca ggggcacctg
      361 ctcgtcgagg gcgtgcccgg tcttgccaag acgctcacgg tgaaaacact cgcgaaggtg
      421 attcgcggct cgttcaagcg catccagttc acgcccgatc tcgtgcccgc cgacctcgtc
      481 ggcacgcgga tctacaacca gaaaacgggc gacttcacga cgtcgctcgg cccggtgttc
      541 accaacctgc tgctggccga cgaaatcaac cgcgcgccgg cgaaagtgca aagcgcgctg
      601 ctcgaggtga tgcaggaata ccaggtcacg atcgccggcc agacccatat ggtgccgacg
      661 ccgttcctcg tcatggcgac gcagaatccg atcgaaaccg aaggcacgta cccgctgccc
      721 gaggcgcagg tcgaccgctt catgatgaag gtgctcgtcg gctatccgag cgaggaggag
      781 gaattcgtca tcgtcgagcg ggtgaccggg cttgccgccg acgtttcgac cgtcgcgagc
      841 accgaccagc tcgccgcact tcagcgccag tgccgcagcg gctacgtcga cccgtcgctg
      901 atccagtacg cggtgcgtct cgcgggcgcg acacgcttcc ccgaccgcta cggcgtcggc
      961 gacttcgcga aatacgtgac gttcggcgcg agcccccgcg cgtcgatcag cctgatcgag
     1021 ggtgcgcgag cgctcgctta cctgcgcggc cgcgattacg tgcttcccga cgacgtcagc
     1081 gatctggccc ccgacgtgct gcgccaccgg ctggtgctgt cgtacgaagc gctggccgac
     1141 gggctcacgc ccgacgacct cgtgcacaag gtgatgcagc gcatcgtgcc gcccgagaag
     1201 ccgctcgaat cgcacgtgaa attcgcgcgg gcatgaacat ccccccacgc ttgcggctgt
     1261 cgccgctgcg ctgccccccg cgggggcgga attcgctatg acgaacgtgg tgccgctcga
     1321 ttccgcgcgc agcgccgagc gcgcgctgcg ccggctcgag tggacggtgc tgcgccgcct
     1381 cgacggcctg ctgcacggcg actaccggac gctcttccgc ggtttcggcc tcgacctcgc
     1441 cgaccttcgc gagtaccagt atcacgacga cgtgcggcac atcgactgga acgtgaccgc
     1501 acggctggcg acgccgtacg tgcgcgaata caacgaggat cgcgacgtca cggcgtggtt
     1561 cctgctggac ttgagcccct cggtcgactt cggctcgggc gacgtgcgca agcgcaacgt
     1621 gtcgaccgac ttcgtcacgg tgctcgcgcg cattctcacg cgacatggca accgcgtggg
     1681 cgccgcgttc tacggcgaga cgatggatac cgtcgtgccg gcggggagcg gacggcggca
     1741 cgtgctgcag atcctgcacc ggatgctgca tcgcccggcg cccgcgcgcg cctcggccac
     1801 caacctgcac gagttcctgc gcgccgcgtt tccggtgatc gcgcggcgct cgctcgtctt
     1861 cgtggtctcc gatttcatca gcgccgcgtc gtgggcggcc gccctcgccc agttcggcga
     1921 gcggcacgag gtcatcgccg tgcgcctcta cgatccgctc gagcacgagc tccccgacct
     1981 cggacttttg acgatgcagg acgccgagac cggtgagcag gtcttcgtcg acacgcatga
     2041 ccgcggcttc cggcgccgct tcgtgaaggc cgccgaggcg cgcgaggcga gtcttcgcgc
     2101 cggcttccgc gatgcgggtg tggacgtgct cgagctgtcg acgagcggcg atctcgccga
     2161 cgcgatcctg cgtttcgccg acctgcgcaa gcgcaggagc cgcctcgccg ccggtgcggt
     2221 gatgccgcgc ggggagcagc agcatgtcgt tcctctggcc tgacttcctc tggctgctgg
     2281 cgctggtgcc ggcgctggtc gggctgtacc tgctcgcgct gcggcgaaag aagcggctcg
     2341 ccgtgcgtta cgcaagcctg tcgatggtgc gcgacgcgct ggcgatgggg caacgggtgc
     2401 gccggcacgt cccgccggcg ctgttcctgg tgggtctcgc gctgatgatc gtcgccatcg
     2461 cgcgtccgac cgcgacggtg aagctgccca cccagcacga gctcgtcatt ctcgcgatgg
     2521 acgtttcggg cagcatgcgc gcggtcgacg tgaagccgac gcggctcgcg gcggcgcagg
     2581 aagcggcgcg cgcgttcatc aacgaccagc cgagcaacgt gaggatcggc atcgtcgcgt
     2641 tcgcgggcac ggcggccgtc gtgcaggcgc cgaccgagaa tcgcgacgag ctgctttcgg
     2701 cgatcgatcg cttcgagctg cagcgcgcca cggcgatcgg cagcggcctc ctcgtgtcgc
     2761 tgaaggcgat ctttcccgat gtcgagttcg atctgcgcgc ttcgaatccc cgtccgcacg
     2821 cgagcgacag gcacggcatg gcgatcgatc agcccaggga cgccgcgaag gagccgccga
     2881 agccggttgc gcccggctcg tacaaatcgg cggtgatcat cctgctcacc gacggccaga
     2941 cgaccaccgg acccgatccg atcgagtcgg cgaagatcgc ggccgagcgc ggcgtgcgcg
     3001 tctacacggt gggcgtgggt accgtgcagg gcgaagtgat cggctgggag ggctggtcga
     3061 tgcgcgtgcg gctcgacgag gattcgctga agcagatcgc gaacgtcacg cgcggcgaat
     3121 atttctacgc gggaacggca accgacctca agaacatcta caaggggatg agctcgaaga
     3181 tggagctcac gaagcagcag accgaggtca ccgcgctgtt cgtcgcggcc gcggcgctct
     3241 tcgtgctgtt tggcgcgggc ctgtcgctcg tctggttcaa ccggctgctt taggacgtgc
     3301 cgatcaccgc gcgctccccg ggctgatgtc gcggcacacc gcgcaggtgt cctggacgcg
     3361 cggcgacgcg cgcttcaccg atgggcgcta cagccgcggg cacgtctggc gcttcgacgg
     3421 cggcgtcgag gtccccgcgt ctgcgtcacc gcatgcggtg cccccaccct ggtcgcgcgc
     3481 cgaagccgtc gatcccgagg aggcgttcgt cgcgtcgatc tcgagctgcc acatgctgtg
     3541 gttcctgtcg atcgcggcga aacgcgatta cacggtggaa gaatacgtcg acgacgccga
     3601 aggcttcctc gagcaggatg ccgaaggccg gatgtcgatg acgcgcgtcg tgctgcgccc
     3661 gcggatcgcg ttcgccgccg cgcgtcccgc gtcgagcgag gctatccagg cgatgcacga
     3721 cgaggcgcat cacgcgtgct atatcgccaa ttcggtgagg acggcgatca gcgtcgaggc
     3781 gcgctgaacc gaaggtcagg tcgtgacgag cttccggtgc gcctgcacgc tcatcaggat
     3841 gccgagcccg atgaacagcg agatcagtgc ggtgccgccg tacgacacca gcggcagcgg
     3901 cacgccgacc acgggcagga tgccgctgac catcgccatg ttgacgaacg cgtaggtgaa
     3961 gaacatcagc gtgatcgcgc ccgacagaag ccgcgcgaac agcgtcgacg cgtgcgcggt
     4021 gatcatcatc gcgcggccga tcaagagcag gtagaggatc agcagcagcg cgttgccgat
     4081 cagcccgaac tcctcgccga acaccgcgaa gatgaaatcg gtatgccgct cgggcaggaa
     4141 gtcgaggtgc gtctgcgtgc cgttcagcca gcccttgccg acgatgccgc ccgagcccag
     4201 cgcgatcgac gcctgcgtcg agtggtagcc cgcgccgagc ggatcgcgcg acggatcgag
     4261 aaacgtcagg atccgctcgc gctggtagtc gtgcagatgc gtccagacca tcggtccgac
     4321 ggccgccccg agtcccacga ggcccacgat caccttccac gacaacccgg cgaggaacag
     4381 gacgtagaag cccgacgcgc cgatcagcag cgccgtgccg aggtccggct gccgcttgat
     4441 caggtacacc ggcaccacga tcaggatcgc ggcgaacacg aaatcgcgcc agccgatgcg
     4501 accttcgaac ttctggaaat accaggcgag catcagcggc agcgcgacct tcatgagctc
     4561 cgacggctga aagcgcgcga caccgagatt cagccagcgc cgcgagccgt tgacgacgac
     4621 gccgaacgtc gcgacgccga tgagcatcgc caccgcgacg acatagagtg gaacggcggc
     4681 gcgcgcgagc gtctgcggcg cgatgttcgc gaacatccac atcagcacga tcgcgaacga
     4741 caggctcatc gcctggctcg tctcgcgcgc cacgctctgg tcggccgccg agaacagcgt
     4801 cacgaggccg acgccgacga tcgcgagcgc agccgcgaac agcatcgagt cgatgcgccg
     4861 cgtcagcact tcccacacgc gtccgatgag ctgaaccatg ccgaaacgaa tgaccctgtc
     4921 ttcagtcgcc ttcgtcgtcg ccggcgccgg gcatgtcctt cgcggcaccc ggctcggatt
     4981 cgccgagcag gtagcgatcg aacaccttgc gcgcgatcgg cgcggccgcc gaggcaccga
     5041 agccgccgtt ctcgacgagc acggcgacgg cgatcgtcgg atggtcggcc ggcgcatacg
     5101 cctcgaacca tgcgtggtcg cgcaggcgct cgtcgacgtg gtgctccgaa tacttctcgc
     5161 ccttgagcga atacacctgc gcggtgcccg tcttgccggc gctgacgtac ttcgcgtcct
     5221 tgaacgcggc cgcgctcgtg ccttccttgt tcacgccgac cagcgcccgc ttgatcatcg
     5281 cgagatccgc gggccggacg tcgagacgcg acgacggctg gctggcgatc tcgcgcgtcg
     5341 cacccgtctt cacgttcgtg atcgacttca cgaggtgcgg cgtgtagccg acaccgtcgt
     5401 tcgcgatcat cgcgatcgcg tgcgcgagct gcatcggcgt gaatgcgttg tagccctggc
     5461 cgatgccggc cgagatcgag tcgccgaggt accacttgcg gtgctcttcg cgataattct
     5521 tcccggcgaa gcgctggcgc ttccattcgc gcgacggcag cacgccggtc aactcgccct
     5581 cgacgtcgat gccggtcttc tgcccgaagc cgagcttgcc gagaaagcgc gccgtgtcgt
     5641 cgatgtcggt gtcgttggcg agcatgtagt agtacgtgtc gcacgactgg acgatcgact
     5701 tgtacatgtc gacggtgccg tggccgcccg gcttgtcgtc gcgaaagcga tgcgcctgcc
     5761 cggggatctg gaaaaagccc gggtcgtaaa tcgtctgcgc cggcgtgcgc ttgcccgacg
     5821 tgagcgcggc aagcgcgaga tacggcttga tcgtcgagcc gggcggatag gcgccgcgca
     5881 gcggccggtt caagagcggc ttgtcgggcg aatcgttgag ctcctgccag tttgcaggat
     5941 cgatgccgtc gacgaaaagg ttcggatcgt agccgggctt cgacacgaac gcgagcacgt
     6001 cacccgacgt cgggtcgatt gcgaccagcg ccccgcggcg gtcgccgaac gctgcctcgg
     6061 ccacctgctg caagcggatg tcgagcgaca gcgtcaggtt gttcccggcg gtcggcggcg
     6121 tgcgccgcag cgtgcgcacg gcgcgtccgc ccgcgtcgac ctcgacgtcc tcgacgccgg
     6181 tcgtcccgtg cagttcgcgc tcgtacgaga gctcgacgcc gaccttgccg atgtaatcgg
     6241 agcccttgta gttggccgtc tcgtcccagg cggcgatgcg ctcgttgtcc ttgtcgttga
     6301 tgcgcccgat gtagccgatg acatgcgagg cgatctcgcc gaacgggtac tggcggaaga
     6361 gccgcgcctt gatgtccaca cccgggaagc ggtagcggtt aacggcgaag cgcgcgacct
     6421 cctcgtcggt cagcttggtg cggatcggca ggctttcgaa agtccgcgat tcctcgagca
     6481 gcttgcggaa gcgcttgcga tcgcgcggct cgacgtcgac gatctgcgac agcttgtcga
     6541 tcgtctcgtc gagattcgcg acgcgcgacg gcgtgatctc gagcgtgtag gccgagtagc
     6601 tttgcgcgag cacgatgccg ttgcggtcgg tgatgacgcc gcgattgggc acgatgggca
     6661 cgatcgcgat gcgattcgtc tcggccagcg tctcgaaatg cgcgtgctgg acgacctgca
     6721 ggtagaagaa gcgcgcgaac agcccgcaga acgcgatgaa cacgagtgcc gcgacgagcg
     6781 agaggcgccg gcggaaatga tagaggtcgc gctcgggatt gcgcagctcc gacgtgccga
     6841 agccgccgct gtcgcgccgg atgaacagca atctccgact cacgggggcc ccacgcgatc
     6901 gcgacctaac gccgcgagcg cgacggccgc tgcggcgtct gcagcagcac cgagacgagc
     6961 ggccacagca atgcgcccac gagcggtggt gcgacatacg tccatttcgg cagcggcgcg
     7021 ccgccgacga aacgcacgag gagcacgagg agggcgcaca gcaccagcag cacggcgacc
     7081 tgcacggcct gctgccacag cgggaagcgc aacacgcgcc ggcggaaaaa ttcggcaccg
     7141 tacgcgagga gggcatacgc gagcgcatgc tggccgaaca gcgtcgcgtc gccgacgtcc
     7201 atcagcagac cgacgagcca tgcgctgccg acgccgacgt agcgcggctc ctggatgcac
     7261 cagtagagca gcacgagcgc gacgaaatcg ggccggtacg tcatcaccgg atcgttgagc
     7321 ggcacgagat tgagcagcag cgccacgaaa atcgacaaca gcacgaacca cggcctggcc
     7381 ggccgcagga tctcctcggg cgcggccggg ccgaacgagc cgacgtgggg gagcaccctc
     7441 attcgcggtc atccctcatt gaggttccat catcgccgtc cgcgcacgcg gccgcccttt
     7501 ttcgcggcct cggcgtcacc cggctcctcg ggccgcgcgg gcatcgacgc cgattgcgac
     7561 agcacgagca ggaattcgct gtggtcgatg ccggcaagcg gcttgcagag gatgcgcgcg
     7621 aacatctgcc cggaatcgcg cacgacgtcg acgatctcgg ccacggcgag tcccggcgga
     7681 tacgtatggt cgaggcccga ggtcacgagc cgatcgccgg ctcgaatgtc ggccgaaggc
     7741 gacgtgaagc gcaactcggg cgagcgtccg gtgccgttgc cgaacagcac gctgcgcgca
     7801 ccgctgcgct cgacctgcac cggcaccgcc tggtcgcggt cggtgatcag cgtcacttcg
     7861 gccatgtacg ggaacacccg cgtcacctgg ccgacgacgc cgctggcatc gatcacgccg
     7921 gcgccggccg caacacccgc actggcgccc ttgtcgacga acaccttctg ggtgaacggg
     7981 tcgcggctcg tatacagcac ctgcacggcg acggccgtgg ccgcatattg cgcgcgtacg
     8041 tcgagcaagg cacgaagccg cgcgttttcc tcctgctcgc gcgtgtaacc ctgcgttgcc
     8101 ggcgcgcgcg ccaccagttc ctgccggagc atcgcatttt cgtccgccaa gcggcgtttc
     8161 gacccgaagt agtcggacac gtacgcaaaa gccgcgccgg gcatttcaag cgcacgctcg
     8221 accgggtaga ggacgatcgc caccgcctgg cgcacgctct cgagatagcg gtagcgggtg
     8281 tcggcgaaca gcagcgcgag cgacaggagg ccgaagaacg cgagccgcgc gagcggactc
     8341 gggccgcgat tgaagaacgc gggcgtatcg acgggaatgg ggcgcatggg tcaacccgca
     8401 ggcatcaact tcgctctcgc gacgtttcgc gccatacacc cgcgacgcga aacgtcagcc
     8461 cggcacggcg agcgtgggcg gatctatcat ctcaatcgct ggtgaagatg gtctgcagct
     8521 tctccatgtt ctcgagcgcc ttgccgcagc cgcgcacgac gcaggtgagc gggtcctcgg
     8581 cgacgacgac cggaagcccc gtttcctcca tcagcaaccg atcgacgtcg cgcaacagcg
     8641 ccccgccgcc cgtcagcacc atgccgcgtt ccgcaatatc cgcgccgagc tcgggcggcg
     8701 tgcgctcgag cgcgcttttc accgccgaca cgatgctgtt caacggatcg gtcagcgctt
     8761 cgaggatctc gttcgacgac acggtgaagc tgcgcggaat gccctcggca aggttacgcc
     8821 ccttcacttc catttccttg acttccgagc cggggaaggc ggagccgatc gtcttcttga
     8881 tgatttcggc ggttgtctcg ccgatcagca tgccgtagtt gcggcggatg tagttgacca
     8941 ccgcctcgtc gaacttgtcg ccgccgacgc ggaccgatcc cgcgtacacc atgccgccga
     9001 gcgaaatgac gccgacttcg gtggtgccgc cgccgatgtc gacgaccatc gatccggtgg
     9061 catcgccgac ggggaggtcg gcgccgatgg cggccgccat cggctcttcg atcaggaaca
     9121 ccttggatgc acccgcgccg agcgccgact cgcgaatggc gcgccgctcg acctgcgtcg
     9181 atccgcaggg cacgcagatg atgatgcggg gcgacggcga gaacagccgc gaatcgagta
     9241 ctttcttgat gaactgcttc agcatctgct ccgtcaccgt gaaatcggcg atgacgccat
     9301 ccttcatcgg acggatcgcg gtgatgttgc ccggcgtgcg accgagcatc tgcttggcgg
     9361 cgagtccgac ctcctggatc accttcttgc cgttcggacc gccctcctgc cggatggcga
     9421 ccacggacgg ttcgtcgagg acgatgccct tgccgcgcac gtaaatgagc gtgttggcgg
     9481 tgccaaggtc aatcgccaga tcgctactga agtagccttt gaatgaatcg aacatgaagg
     9541 atgtggacgc cggcggcgcg gcgcgtgcct tcccgacagc tcggcgcgca ccgctttgaa
     9601 atggccgata tgataaccta agccgctgtt ctctccgcac tgattcgacg cggttttttg
     9661 cgccgaacgc cactttccgc cgcgctcgag tcttcgatga gcttctcgct tgacgacgtc
     9721 gcgcgccttg cgcgtctcgc ccgcatcgac ctcgacgcgg acacggtccg cgacgtgcgc
     9781 gacaagctcg acgcgatctt cgcgctgatc gacgcgctgc agtcgatcga cacggcaggc
     9841 gttgcgccga tggcgcacgc gcaggacatc atgttgccgc tacgcgacga cgccgtcacc
     9901 gccggcgatt gccgcgaact ctatcaggcg caggcgccgg cggtcgccgg cgggctctat
     9961 ctcgtgccga aggtgatcga gtgactgccg cgccggcacc gggcgcgtat cgcgcggcca
    10021 cgatcggcga gctcgcgcgg gcgcttcgcg gacgcgagct atcgagcgtc gagctcgcgc
    10081 agcagacgct gtcgcgcatc ggcggctcgc aagcggcgct gaacgcgttc atcaccgtcg
    10141 acgccgaccg tgcgcttgtc gaggcgcgcc gcgccgacgc ggcgctcgcc cgcggcgacg
    10201 cgagtccgct caccggcatt ccgatcgcgc acaaggacgt gctgatgacg gccggattgc
    10261 gcacgacctg cgcgtcgcga atgctcgcga atttcgtcgc cccgtacgat gcgcatgtcg
    10321 tcgcccggtt gcgcgaggcc ggtaccgtgc tcgtcggcaa gacgaacatg gacgagttcg
    10381 cgatgggctc gtcgagcgaa acgtcgcatt tcggaccggt acgcaatccg tggagcgccg
    10441 cctgcgttcc cggcggcagc tccggcggct cggcggccgc cgtcgcggcg gggctgcttc
    10501 cggcaacgac cggcaccgac accggcggct cgatccgcca gccggccgcg ctcaccggcg
    10561 tcagcggcct caagcccacg tacggcgtgt gctcgcgcta cgggttgatc gcgttcgcct
    10621 cgagcctcga cacgccgggc cccttcgccc gcaccgccga ggactgcgcg ctgctgctca
    10681 acgcgatggc aggtcacgac gaacgtgact gcacttcgct cgatcggccg cgcgagaatt
    10741 acctgcgcgc gcttcatgcg ccggcaacgg cgcggccgct tgccggcgtg cggatcggcc
    10801 tgccgcgcga atatttcacc gacggactcg agcgcgaggt cgcgagcgca gtcgacgcgg
    10861 cactcggcga gttgcgcgcg ctcggcgcga cgaccgtgga catcgacctg ccgatggtca
    10921 ggcatgccgt cgccgtctat tacgtcattg cgccggccga agcatcgtcg aacctgtcgc
    10981 ggttcgacgg cgttcgctat ggtcatcgcg ccgcgcggta cagcgacctg accgacatgt
    11041 attgccgcac gcgcgccgaa ggtttcggcg cggaggtgaa gcggcgcatt ctcgtcggca
    11101 cgtacgtgct gtcgcacggc tactacgacg cgtactacct gaaggcgcaa caggtgcgcc
    11161 ggctgatcgc gcaggacttc ctgcgcgcgt tcggcgaatg cgacgtgatc gccggaccga
    11221 ccgcaccgac gtgcgccttc gcgatcggcg ccaaggccga cgatccggtg cagatgtacc
    11281 tgaacgacat cttcacgatc gccgcgaacc ttaccgggat gcccgccatc tccattccct
    11341 gcggattcac gcgcgatgcc ttgccgatcg ggctgcagct ccagggcaat cacttcgacg
    11401 aggcgcggct tctgaacgtc gcgcatcgct tccagcaggc gaccgactgg catttgcgct
    11461 gcccctcacc ccaaccctct cccgacgcat gaactgggaa atcgtcatcg gcatcgagac
    11521 ccacgcgcag ctttcgacgg tgtcgaagat attctcgggc gccgcgacgg cgttcggtgc
    11581 cgcgcccaac acgcacgcaa gcgccgtcga cattgcgctg cccggcgtgc tgccggtgcc
    11641 gaaccgcggc gcggtcgacc gggcgatccg tttcggcctc gccgtcggcg gcacgatcaa
    11701 tcggcgcagc gtgttcgcgc ggaagaatta cttctatccc gacctgccga agggctacca
    11761 gatctcgcag tacgaaatcc ccgtcgtgca gggcggcacc gtgaccatcg ccaccggcgc
    11821 gggcgagcgg caggtgcggc tcacgcgcgc gcatctcgag gaagacgcgg gcaagtcgct
    11881 gcacgagggc ctgccacgcg agctcggcga tgtcaccggc atcgacctca atcgcgccgg
    11941 cacgccgctg ctcgagatcg tatcggagcc cgacatgcgc agcgcggagg aagccgtcgc
    12001 ctacgcgaag gcgctgcatt cgctcgtcac gtggatcggc atctgcgacg gcgacatgca
    12061 ggagggcaac tttcgctgcg acgcgaacgt atcggtgcgc cgcaccggcg agccgctcgg
    12121 cacccgctgc gagatcaaga acctcaacag cttccgcttc ctcgcgcagg cgatcgagca
    12181 cgaggcgcgg cgccagatcg agctgatcga ggatggcggg cgcgtcgtgc agcagacgcg
    12241 cctttacgat ccggatcgca acgagacgcg gccgatgcgc agcaaggaag atgcgcagga
    12301 ctatcgctat tttcccgatc ccgatctgcc gccgctgacg atcgacgacg catggatcga
    12361 gcgtgcgcgc gccgccttgc cggagcttcc cgccgcgatg cgcgcgcggc tcgtcgcgca
    12421 gcacggcatc accgattacg acgcggcgca gctgacggcg tcacgcgatc tcgtcgacta
    12481 ctacaacgcg gtgtgcgccg cgctgcccgc ggccgacgcc gcttcgcaca agcacgtcgc
    12541 gaacttcgtg ctcggcgaat tcgcagccgc gcgcaatcgc gacgacatcg cggtgcacgc
    12601 atcgccagtc gcgccggagc agctcgctga gttgctacgg cggctccagg atgacacgct
    12661 gtctcggatg aaggcgaagg acgtattcga cgagatgtgg aaccgaagtc gcaacgtttc
    12721 tgttcagctc accggcgccg aaatgagagc tacgtcggga atggtggatg ccatcatcga
    12781 gcaacgcggg ctgcgccaga tttcggatgc cgcggccatc gaaaccttgg tcgagcaggt
    12841 gattgc
//
LOCUS       DASSVX010000024        11725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_884080, whole genome shotgun sequence.
ACCESSION   DASSVX010000024 DASSVX010000000
VERSION     DASSVX010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 11725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11725
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884080"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFO53_01145"
     CDS             complement(<1..527)
                     /locus_tag="VFO53_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET8874907.1"
                     /translation="MSVYTAVTADELDAWLARYALGTLVEHSPIAAGIENSNYFVTTE
                     RGRYVLTLYERLPADELPFYLNLMAHLARSGVEVPAPAPDRTGALFSHLNGRPAGLVS
                     RIDGVAVERPTPAHCASVGNALARLHVAAQSYRARLANRRGPAWWRQAARAVRPYLTA
                     EQNDLLAAEVRFLTG"
     gene            complement(656..973)
                     /locus_tag="VFO53_01150"
     CDS             complement(656..973)
                     /locus_tag="VFO53_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HET8874908.1"
                     /translation="MLLASMIAGWSFAQAPARTEPLPPPPPPAASVLDDADVEPQVTI
                     LRTDEQTTEEVRVQGQLRYVRVTPRYGRPYYLIPDANGATYIRRDSLDSTLKVPMWVL
                     FSF"
     gene            complement(1171..1899)
                     /locus_tag="VFO53_01155"
     CDS             complement(1171..1899)
                     /locus_tag="VFO53_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET8874909.1"
                     /translation="MRLQTHKIPDLIDPQALYSTNGQEAWRVLGIMAEFQMAADRLNG
                     VRPAVSMFGSARIKDGHPYYATAKIIARKLSDAGFSVISGGGPGLMQAANHGAHAGRA
                     PAIGLNIVLPNEHASNQYQDISVDFRYFFARKMMFVRFASAYVVLPGGFGTLDELTEC
                     LTLVQTGKSRRIPIILVGGSFWQGFLDWLDDKVAAEGMIGENDVGLMRVIDEPDAIVE
                     AIFNFYENRGFQPSRDEREKLLNL"
     gene            1898..4633
                     /gene="polA"
                     /locus_tag="VFO53_01160"
     CDS             1898..4633
                     /gene="polA"
                     /locus_tag="VFO53_01160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET8874910.1"
                     /translation="MATLVLVDGSSYLYRAFHALPDLRTSKGEPTGALRGVLSMLRRL
                     VIDDKPDYFAVVFDAPGKTFRDDWYADYKANRATMPDDLTLQITPLHELVRAHGWPLV
                     MVEGVEADDVIGTLARQAKAAGIETTISTSDKDLAQLVEPGVTLVNTMTNERLDHEGV
                     VAKFGVRPDQVLDLLTLTGDAIDNVPGVAKVGPKTAAKWLAQYESLDNLIARAHEVGG
                     VVGENLRSALDWLPQGRRLLTVKVDCELASKVADLTLGEPELDRLRGLYERYEFKSWL
                     RDLGRSDVNVEIAPKPASGSASREEARDAPRPAPRTGDETVTSIDALERWLAAVAAAP
                     IVAIDVETTHADPMTAGIVGIALATAAGRAAYVPLDHRYAGAPAQLPRDDVLGRLAAW
                     LGDAARPKLGHDLKFAEHALANHGIALRGVAHDTLLESYVLESHKPHELAVLALRHLD
                     VAITGYDALTGRGANRIPFEQVAVDTATAYSAEQADITLRLHRHLFDAIERDEKLRHI
                     YELIELPVRDVLFRMERNGVLVDRDLLAAQSRELGERAQALEAEAHRLAGTPFNLGSP
                     KQLCEVLFERMRLPVKKKTATGQPSTDEEVLQELAADYPLPKVLLEYRALTKLKSTYT
                     DKLPAMINAQTGRVHTTFSQATAVTGRLASLEPNVQNIPVRTAEGRRIREAFVAAPGH
                     TIVSADYSQIELRIMAHLSEDPALLAAFHGDADIHRVTAAEIFSVAPEEVSADQRRYI
                     KAVNFGLIYGMSAFGLAAQLNIERAAAAQFIDRYFTRYPGVATYMQRTRELAREQGYV
                     ETVFGRRLFLPDIRAASGPRRAGAERAAINAPMQGTAADLIKLAMIAVQAWLDRERLG
                     TRLILQVHDELVLEAPDSEVPRIVRELPALMTSVATLRVPLKVDIGHGPNWEKAH"
     gene            complement(4662..5447)
                     /locus_tag="VFO53_01165"
     CDS             complement(4662..5447)
                     /locus_tag="VFO53_01165"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HET8874911.1"
                     /translation="MEIDPPASARADDAEGPAPPPRLLAAGDGAAWWGESWRIFCAAP
                     FAWVGIFVVFVVLSIALVMVPVVGSLVHTVLTPVFAGGVMLGCHALARGRPLTIGHLF
                     EGFQRGRFAPLLTLGLIWLALLIVIAVVMVASLFLTLGASGLAALMDMGGSTPIDYTA
                     LLSAGMAMFVVVIVALIVSLLAAMAYWFAPALVVLNGETPMDALSKSFTASWHNVGAF
                     LLYGLVFIGLAIAASIPLALGWFVLGPMLVGSCYAGWRTIFGR"
     gene            5594..6553
                     /locus_tag="VFO53_01170"
     CDS             5594..6553
                     /locus_tag="VFO53_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET8874912.1"
                     /translation="MDSIAGELARYGVPLVGLNVLLQQLGLPIPAVPTMMLAGALAMA
                     GRIDLLAAFAVAVLASLVADLLWYWAGRRYGYPVLRFLCRISLSPDTCVRQTEGIFER
                     WGFYSVVVSKFVPGFATVAPPIAGALSMRVGAFTLASAASAALWAGAAMATGALFAAQ
                     IDRALAWMASHAALAALAVAGLIVLYALVKAWQRWRMARLLAGAMISVDELRERLAVE
                     PRPFVVDVGSSLAQGTRAHIPGAVLLDLDAISRCDDFPADRDIVLYCACPNEASARRG
                     AQILLSKGYRRVRPLRGGIEAWIAAGHAVDRTLPVTFAARAAA"
     gene            6762..8105
                     /locus_tag="VFO53_01175"
     CDS             6762..8105
                     /locus_tag="VFO53_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET8874913.1"
                     /translation="MKPPPPAPATRATGDRPAVSRRGRRWGSVLALIVLAALAALAWY
                     LTHRPVAIAPGTPGPAGAAGARGGSAARGGASGARSAPPSTVGTATARHADIPVFIDA
                     LGTVTPIAVATVSPQVSGVITEVLYQEGGMVKKGQALATIDPRPFQMALDQASAARVR
                     DEAQLNAARVQLERYQVLLKQDSIARQTVDTQDALVKQLAGTVGVDRANENSARLNLT
                     WSRIVAPIAGRIGLRPIDVGNYIAAGNATGVATITQVSPIDVQFSIPQERVPDVKERL
                     AGGAKLAVTAWDSGRLRKLDDGEFLTLDNQIDVQTGTIKAKARFANAGGALFPNQFVN
                     ARLLLRTIDAAVVVPVIALRHGPSGDFVYIVNADHTVSLRNVTAGVSGVDDVEITKGL
                     AVGDVVVTEGGDRLKDGARIQTSADRPAGATPAPPPGTEAGRRGRGPRTKDGSGT"
     gene            8112..11378
                     /locus_tag="VFO53_01180"
     CDS             8112..11378
                     /locus_tag="VFO53_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8874914.1"
                     /translation="MSPSRPFIERPVATSLLMLAIVLAGLVAFRFLPLSALPQVDYPT
                     IQVQTLYPGASPEVMSNTVTAPLENQFGQMSGLDRMSSTSASGVSVITLQFSLDLSLD
                     VAEQEVQAAINASGSLLPADLPAPPVYAKVNPADAPVLTLAVSSESLPLTDVQNLVNT
                     RLAQKMSQVPGVGLVSLSGGQRPAVRIQANTQALASYGIGLDTLRTAISNANSNAAKG
                     NFDGPKRAYSISANDQLLSADDYRQLIVAYRNGAPVRMASVADVVESAENNQLGAWAG
                     FPCPARPAGGLAAAELPGNDCRGNGGAVLIPAIILNVQRQPGANVIATVDAIKAQLPV
                     LQAGLPASIRVDVLADRTSGIRASVSDVEKELVLAVVLVVLVIFAFLGSLRATVIASI
                     AVPISLIGTFGLMYLLGYSLNNLSLMALTIATGFVVDDAIVMIENISRYIEQGEPPLR
                     AALTGATQIGFTIISLTISLIAVLIPLLFMSDVVGRLFREFAVTLAITILISAVVSLT
                     LVPMMSARWLRTRRDDAEAKLSRAQRFFARVMDRYDRSLVFVIDHQAATLVVAVATLV
                     VTALLYVVIPKGLFPTQDTGQLKARIQAAQDVSYARMADVQAQAARAILADPAVANLS
                     SIVGVDAANNTMLSTGSMLINLKPSHGDQQAVMNRLRERVRAVPGVTLYLQPTQDLTI
                     DAETGPTEYRASLEGVDTAQITRWMQLLIARLQDAPEVRNVSSDAGAQGATTHIAVDR
                     DTASRLGITASAVDDALYSAFGQRIVSTIFTDTNQYRVILEAKPGLATSAEGVGRLNL
                     ISSSGKPTPLSAFAKITEQRAPLQITHVGQYPASTLNFDTAPGVALGHAVDAIRTAAH
                     DIGLPPQITMTFLGASGAYERSLANQLWLILAAVVCVYIVLGVLYESYVHPLTILSTL
                     PSAGVGALLALIITGNDLGVISIIGIILLIGIVKKNAIMMIDFAIDAEREQGMSARDA
                     IHQAALLRLRPILMTTLAALFAAVPLMLSWGVGAELRRPLGLAIFGGLIVSQLLTLFT
                     TPVIYLAFDRLGGGSTRRKMADEMLAGAATSGEGPSRAASSAPAGGSAAAKPQAWGDV
                     "
     gene            11375..>11725
                     /locus_tag="VFO53_01185"
     CDS             11375..>11725
                     /locus_tag="VFO53_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8874915.1"
                     /translation="MNLSRPFVLRPIGTVLLTAGVALAGIAGFFVLPVASLPQIDLPV
                     ISVSANIPGASPETMAQSVATPLERHLGTIPSVNEITSTSSVGATRVTLQFDLGRDID
                     GAARDVQAAINASRV"
BASE COUNT         1889 a         4033 c         4003 g         1800 t
ORIGIN      
        1 aagccggtca ggaatctgac ctcggcggcg agaagatcgt tctgctcggc ggtgaggtag
       61 ggacgcacgg cgcgcgccgc ctggcgccac caggccgggc cgcggcgatt ggcgaggcgt
      121 gcgcggtagc tctgcgcggc aacgtgcagg cgcgcgagcg cgttgccgac cgacgcgcag
      181 tgcgcaggcg taggccgctc gacggcgacg ccgtcgatgc gcgacacgag ccccgccggg
      241 cgcccgttca gatgcgaaaa gagcgcaccg gtccgatcgg gcgccggtgc cggcacttcg
      301 acgccggacc gcgcgagatg cgccatcagg ttgaggtaga acggcagctc gtccgccggc
      361 aggcgctcgt agagcgtcag cacgtagcgg ccgcgctcgg tggtcacgaa atagttgctg
      421 ttctcgatgc cggcggcgat cggcgaatgc tcgacgagcg tgccgagcgc gtaacgtgcg
      481 agccacgcat ccagttcgtc ggcggtgacg gcggtataaa cggacatgaa aacgcatgcc
      541 gcacgcgacg cgcggcatgc ccaaagcgcg atgtcagcaa acgaggatca acggaaaact
      601 gcgaaacgca tcaccgccgg accactcccg aggccccgca tgcgcgattg tccacctaga
      661 acgaaaacag cacccacatc ggaaccttga gggtcgagtc gaggctgtcg cggcggatgt
      721 aggtggcgcc gttggcgtcg gggatcagat agtacggccg gccgtatctc ggcgtcacgc
      781 gcacgtagcg cagttgccct tgcacgcgca cttcctcggt ggtctgctcg tcggtgcgaa
      841 ggatcgtcac ctgcggctcg acatcggcgt cgtcgagcac gctggcagcc ggcgggggcg
      901 gcggcggcag cggctcggtc cgggcgggcg cttgcgcgaa gctccagccg gcgatcatgg
      961 aagcgagcag catcagggca gggcggcgtg catccatggc gagctcggaa ggagtttgga
     1021 agcggcagtg ttcagggaag cgaagacagt gtcattctag caaacggccg agcatcaacg
     1081 cattgcctgc gccgtgatcc ggcgtcgtcc accgccgata tcgatgtcgg tgccacgcct
     1141 acgccggcca tgcaggcttc gagcggtgca tcaaaggttc agaagcttct cgcgttcgtc
     1201 gcgcgacggc tggaagccgc ggttttcgta gaagttgaag atcgcctcga cgatcgcgtc
     1261 gggctcgtcg atcacgcgca tcaggccgac gtcgttctcg ccgatcatcc cttccgcggc
     1321 aaccttgtcg tcgagccagt cgaggaagcc ctgccagaac gagccgccga cgaggatgat
     1381 cgggatgcgc cggctcttgc ccgtctgcac cagcgtcagg cattcggtca gctcgtcgag
     1441 cgtgccgaag ccgccgggca gcacgacata cgccgacgcg aagcgcacga acatcatctt
     1501 gcgcgcaaag aagtagcgga agtcgacgct gatgtcctgg tattggttgc tcgcgtgctc
     1561 gttgggcagc acgatgttga gcccgattgc gggcgcgcgg cccgcgtgcg cgccgtgatt
     1621 ggccgcctgc atcaatcccg gaccgccgcc cgagatcacc gagaagcccg cgtcggacag
     1681 tttgcgcgcg atgatcttcg cggtcgcgta atacggatgg ccatccttga tgcgcgcgct
     1741 gccgaacatg ctgacggccg gacgcacgcc gttcaggcga tcggccgcca tctggaactc
     1801 tgccataatc cccagcaccc gccacgcttc ctgtccgttc gtcgaataca gcgcctgcgg
     1861 gtcaatcaga tcgggaatct tgtgggtttg taaccgcatg gcaacgctcg tcctcgtgga
     1921 tggctcgtct tatctgtacc gcgcgttcca cgcgctgccg gatctgcgca cgtcgaaggg
     1981 cgagccgacc ggcgcgctgc gcggggtgct gtcgatgttg cgccgtttgg tcatcgacga
     2041 caagcccgac tatttcgccg tcgtgttcga tgcgccgggc aagacgttcc gcgacgactg
     2101 gtacgccgac tacaaggcga accgcgcaac gatgcccgac gatctcacgc tgcagatcac
     2161 gccgctgcac gagctcgtgc gcgcgcatgg ctggccgctg gtgatggtcg aaggcgtcga
     2221 ggccgacgac gtgatcggca cgctggcgcg acaggcgaaa gcggcgggca tcgaaacgac
     2281 gatctcgacg tccgacaagg acctcgcgca actggtcgag cccggcgtca cgctcgtcaa
     2341 cacgatgacg aacgagcgtc tcgatcacga aggcgtcgtc gccaagttcg gcgtgcgccc
     2401 cgaccaggtg ctcgacctgc tcacgctcac cggcgatgcg atcgacaacg tgccgggcgt
     2461 cgcgaaggtc ggcccgaaga cggccgccaa gtggctcgcg cagtacgagt cgctcgacaa
     2521 cctgatcgct cgcgcgcacg aggtcggcgg cgtcgtcggc gagaacctgc gcagcgcact
     2581 cgactggctg ccgcagggac gccgattgct cacggtgaag gtcgattgcg agcttgcgtc
     2641 gaaggtcgcc gacctcacgc tcggagagcc cgagctcgat cggctgcgcg ggctgtacga
     2701 gcgctacgaa ttcaagagct ggctgcgcga tctcggccgc agcgatgtca acgtcgagat
     2761 cgcgccgaag cccgcctcgg gcagcgcgag ccgcgaggaa gcgcgcgacg cgccgcggcc
     2821 cgcgccgcgc accggcgatg aaacggtcac gtcgatcgac gcgctcgagc gctggctcgc
     2881 ggcagttgcg gcggcgccga tcgtcgcgat cgacgtcgag acgacccatg ccgatccgat
     2941 gacggccggg atcgtcggca tcgcgcttgc caccgccgcc ggccgcgccg cttacgttcc
     3001 gctcgaccat cgctacgcgg gcgcgcccgc gcagcttccg cgcgacgatg tcctcgggcg
     3061 tcttgccgcg tggcttggcg acgccgcgcg gccgaagctc gggcacgatc tcaagttcgc
     3121 cgagcacgcg ctcgccaatc acggcatcgc gctgcgtggc gtcgcgcacg acacgctgct
     3181 cgaatcgtac gtgctcgaat cgcacaagcc gcacgaactc gcggtgctcg cgctgcgcca
     3241 tctcgacgtt gcgatcaccg gctacgacgc gctcaccggc agaggcgcga accgcatccc
     3301 gttcgagcag gtcgccgtcg acacggccac cgcgtactcg gccgagcagg ccgacatcac
     3361 gttgcgcctt catcggcatc tcttcgacgc aatcgagcgc gacgaaaagc tgcgccatat
     3421 ctacgagttg atcgagctgc cggtgcgcga cgtgctgttt cgcatggagc gcaacggcgt
     3481 gctggtcgac agggacctgc tagccgcaca gagccgcgag ctcggcgagc gcgcgcaggc
     3541 gctggaggcc gaggcgcatc ggctcgcggg caccccgttc aatctcggct cgccgaagca
     3601 gctttgcgag gtgctgttcg agcggatgcg gctcccggtg aagaagaaga ccgcgaccgg
     3661 ccagccgtcg accgacgaag aggtgctgca ggagctcgcg gccgactatc cgctgccgaa
     3721 ggtgctgctc gaataccgcg cgctgaccaa gctcaagtcg acgtacaccg acaagctgcc
     3781 ggcgatgatc aatgcgcaaa ccggccgcgt gcacacgacg ttctcgcagg cgaccgcggt
     3841 gaccgggcgg cttgcgtcgc tcgagcccaa cgtgcagaac attccggtgc gcaccgccga
     3901 aggacggcgc atccgcgaag cgttcgtcgc ggcgccgggt cacacgatcg tctccgccga
     3961 ttattcgcag atcgagctgc gcatcatggc gcacctgtcg gaggatccgg cgctcctcgc
     4021 cgcgtttcac ggcgacgccg acatccaccg cgtcaccgcg gcggagatct tcagtgtcgc
     4081 cccggaagag gtgtcggccg atcagcgccg ctacatcaag gcggtcaact tcgggctgat
     4141 ctacggaatg agcgcattcg ggctcgccgc ccagctcaac atcgagcgcg cggccgccgc
     4201 acagttcatc gaccgctatt tcacgcgcta tccgggcgtc gcgacgtaca tgcagcgcac
     4261 gcgcgagctg gcgcgcgagc agggctacgt cgaaacggta ttcgggcggc gcctgttcct
     4321 gcccgacatc cgcgccgcaa gcgggccgcg gcgggcgggt gcggaacgcg ccgcgatcaa
     4381 cgcgccgatg cagggcacgg ccgccgacct gatcaagctc gcgatgatcg ccgtgcaggc
     4441 gtggctcgac cgcgagcgtc ttggcacgcg gctcatcctg caggtgcacg acgagctggt
     4501 gctcgaggcg cccgatagcg aagtgccgcg gatcgtgcgc gagctgccgg cgttgatgac
     4561 gtcggtggca acgctacgcg tgccgctgaa ggtggacatc ggccacggac ccaactggga
     4621 aaaggcgcat tagaagctgc tgcgcgcccc gattgcctgc gtcagcgtcc gaagatcgtc
     4681 cgccatcccg cgtagcagct gccgacgagc atcggcccca gcacgaacca gccgagcgcg
     4741 agcgggatcg acgccgcgat ggcgagcccg atgaacacga ggccgtagag caggaatgcg
     4801 ccgacgttgt gccacgatgc ggtgaagctt ttcgaaagcg catccatcgg cgtctcgccg
     4861 ttcagcacga cgagcgccgg cgcgaaccag tacgccatcg ctgcgagcag cgagacgatc
     4921 agcgcgacga tgacgacgac gaacatcgcc atgccggccg acagcagggc cgtgtaatcg
     4981 atcggcgtcg acccgcccat gtccatcaac gccgcgagcc cggaggcgcc gagcgtcagg
     5041 aacaaggacg cgaccatcac gaccgcgatc acgatcagga gggcgagcca gatgagcccg
     5101 agcgtcagaa gcggcgcgaa gcggccgcgc tggaagcctt cgaacagatg accgatcgtg
     5161 agcggccggc cgcgcgcgag cgcgtgacag ccgagcatca cgccaccggc gaataccggc
     5221 gtcagcaccg tgtgcacgag gctgccgacg accgggacca tcacgagcgc gatcgacagc
     5281 acgacgaaga cgacgaagat gccgacccac gcgaacgggg ccgcgcaaaa gatgcgccag
     5341 ctttcgcccc accacgccgc gccgtcgccg gcggcgagaa gacgtggcgg cggcgccggg
     5401 ccttcggcgt cgtcggcgcg ggcggacgcg gggggatcga tttccatggt ttcgtcgggc
     5461 cgggcaggcg cggtcggcag cgcgtgcggc ggggacccga ttgtcgcgta cccggccgca
     5521 aaagacgagc gggacttggg aaccggcgtc ggcgctccca tctcgcaatt tccccgcatt
     5581 gaccggaaat cggatggatt cgatcgcagg cgagcttgcc cgatacggtg tccccctggt
     5641 cgggctgaac gtgctgctgc agcagctcgg cctcccgatc ccggcggtgc cgacgatgat
     5701 gctggccggt gcgctcgcga tggccggtcg catcgacctc ctcgccgcat tcgcggtcgc
     5761 ggtgctggcc tcgctcgtcg ccgatctcct gtggtactgg gccggacgcc gctacggcta
     5821 tccggtgctg cgcttcctgt gccgcatttc gctgtcgccc gacacctgcg ttcgccagac
     5881 cgaaggcatc ttcgagcgct ggggcttcta ttcggtggtc gtctccaagt tcgtgcccgg
     5941 cttcgcgacg gtggccccgc cgatcgccgg cgcactgtcg atgcgcgtcg gcgcgttcac
     6001 gctggcatcg gcggccagcg cggcgctgtg ggccggtgcc gcgatggcaa ccggggcgct
     6061 gttcgcggcg cagatcgatc gggcactcgc gtggatggcg tcgcacgcag cgctcgcagc
     6121 gctggccgtt gccggcctga tcgtcctgta cgcgctcgtc aaggcatggc agcggtggcg
     6181 gatggcgcgg ttgctcgccg gcgcgatgat ctcggtggat gagctgcgcg agcggctcgc
     6241 ggtcgagccg cggccgtttg tcgtcgatgt cggctcgtcg ctcgcgcagg gtacgcgggc
     6301 gcacattcca ggcgcggtgc tgctcgatct cgacgcgatc tcgcgttgcg acgatttccc
     6361 ggccgaccgc gacatcgtcc tctattgcgc gtgcccgaat gaggcatcgg cgcgccgggg
     6421 cgcgcagatc cttctgtcga agggctatcg ccgggtccgg ccgctgcgcg gcggcatcga
     6481 ggcgtggatc gcggcggggc acgcggtcga ccggactctc ccggtcacct tcgccgcgcg
     6541 ggcggccgcc tgaaacgatt ccttgcaatt cgcgacgcgc attcacggca tactgccggc
     6601 gcctccgaga accgatcctg caggatcgcc gtctgatttg ccgtcgcgtt agcgggagac
     6661 ccccgcggcg cgatcgtcgc gcgcgatccg gaagccgatc ggccgcatcg acggtcggcc
     6721 accctttcaa ccacgacaga cccggctttc agggaccttc gatgaaacct ccgccgcccg
     6781 cccccgccac gcgagcaacc ggggaccgcc cggccgtatc acgccgcggc cggcgctggg
     6841 gcagcgtcct tgcgctgatc gtgctcgccg cgctcgccgc actcgcgtgg tacctgacgc
     6901 accggccggt cgcgatcgcg cctggcacgc ccggacccgc cggtgcagcc ggcgcacgcg
     6961 gtggcagcgc ggcgcgcggc ggcgcgtcgg gtgcgcgcag cgcgccgccc agcacggtcg
     7021 gcaccgccac ggcgcgccac gccgacattc cggtcttcat cgatgcgctc ggcacggtga
     7081 cgccgatcgc cgtcgccacg gtatcgccgc aagtctcggg ggtgatcacc gaggtgctct
     7141 accaggaagg cgggatggtg aaaaagggcc aggcgctggc gacgatcgat ccgcgtccat
     7201 tccagatggc gctcgaccag gcgagcgcgg cgcgggtacg cgacgaggcg cagctcaatg
     7261 cggcccgcgt gcagctcgag cgctaccagg tgctgctgaa gcaggattcg atcgcccgcc
     7321 agaccgtgga cacgcaggat gcgctggtga agcagcttgc cgggaccgtg ggcgtcgatc
     7381 gcgcgaacga gaacagcgcg cggctcaacc tgacgtggtc gcgtatcgtt gcgccgatcg
     7441 cgggtcgcat cggcctgcgt ccgatcgacg tcggcaatta catcgccgca ggcaatgcca
     7501 ccggcgtggc gacgatcacg caggtgtcgc cgatcgacgt ccagttctcc attccgcagg
     7561 agcgcgttcc ggacgtgaag gagcgcctcg ccggcggtgc gaagcttgcc gtgacggcgt
     7621 gggacagcgg tcgcctccgc aagctcgatg acggcgagtt cctgacgctc gacaaccaga
     7681 tcgacgtgca gaccggcacg atcaaggcga aggcgcgctt cgcgaatgcc ggcggcgcgc
     7741 tgttcccgaa ccagttcgtc aacgcgcgcc tgctgctgcg cacgatcgac gcggccgtcg
     7801 tcgtgcccgt cattgcgttg cgccacggtc cgagcggcga tttcgtctat atcgtcaacg
     7861 ccgaccatac cgtttcgctg cgcaacgtga cggcgggggt ttccggcgtc gacgacgtcg
     7921 agatcacgaa ggggcttgcc gtcggcgacg tcgtggtcac tgaaggcggt gaccggctga
     7981 aggacggcgc gcgcatccag acgagcgccg atcgtcctgc cggtgcgact cccgcgccgc
     8041 ccccgggcac cgaggcggga cgtcgcggac gcggtccgcg caccaaggac gggtccggga
     8101 cgtaaagcca agtgtctcca tcgcgcccct tcatcgagcg gccggtcgcg acatcgctgc
     8161 tgatgctcgc gatcgtgctc gcgggcctcg tcgcgtttcg cttcctgccg ttgtcggcgt
     8221 tgccgcaggt cgactacccg acgatccagg tgcagacgct gtacccgggc gcgagccccg
     8281 aggtgatgtc gaataccgtc accgccccgc tcgagaacca gttcggtcag atgtctggcc
     8341 tcgaccggat gagctcgacg agcgcgtcgg gcgtgtcggt gatcacgctc cagttctcgc
     8401 tcgacctgtc gctcgacgtc gccgagcagg aggtgcaggc ggcgatcaac gccagcgggt
     8461 cgctgctgcc cgccgacctg cccgcgcctc ccgtctatgc gaaggtgaac ccggccgacg
     8521 cgccggtgct gaccctcgcg gtcagctccg agagccttcc gctcaccgac gtgcagaacc
     8581 tcgtgaacac ccggctcgcg cagaagatga gccaggtgcc gggcgtcggg ctcgtgtcgc
     8641 tgtccggcgg gcagcggccg gcggtgcgca tccaggcgaa cacgcaggcg ctcgcgtcgt
     8701 atggcatcgg cctcgacacg ctgcgcacgg cgatcagcaa cgcgaactcg aacgccgcca
     8761 aggggaattt cgacgggccg aagcgcgcct attcgatcag cgcgaacgat cagctgctga
     8821 gcgccgacga ttaccggcaa ctgatcgtcg cgtatcgcaa cggcgcgccg gtccgcatgg
     8881 cgagcgtcgc cgatgtcgtc gaaagcgccg aaaacaacca gctcggggca tgggccggct
     8941 tcccgtgccc ggcgcgcccc gccggcggcc tcgccgcggc cgagcttccc gggaacgact
     9001 gccgcggcaa cggtggcgcg gtgctgattc ccgcgatcat cctcaacgtg cagcgccagc
     9061 cgggcgcaaa cgtgatcgcc accgtcgacg cgatcaaggc gcaactgccc gtgctgcagg
     9121 ccggcctgcc ggcgtcgatc agggtggacg tcctcgccga tcgcacgagc ggcatccgcg
     9181 cgtcggtgag cgacgtcgag aaggagctcg tgctcgccgt cgtgctggtc gtgctcgtga
     9241 tcttcgcctt tctcggcagc ctccgcgcaa cggtgattgc gagcatcgcc gtgccgatct
     9301 cgctgatcgg aacgttcggg ctcatgtacc tgctcggcta cagcctcaac aacctgagcc
     9361 tgatggcgct gacgatcgca acgggcttcg tcgtcgacga cgcgatcgtg atgatcgaga
     9421 acatctcgcg ctacatcgag cagggcgagc cgccgcttcg cgcagcactg accggcgcga
     9481 cgcagatcgg cttcacgatc atctcgctga cgatctcgct catcgccgtg ctgatcccgc
     9541 tcttgttcat gagcgacgtc gtcgggcgcc tgttccgcga attcgccgtg acgctcgcga
     9601 tcacgatcct gatctcggcc gtcgtgtcgc tgaccctggt gccgatgatg tcggcgcggt
     9661 ggctgcgcac ccggcgcgac gacgccgagg cgaagctctc ccgggcgcag cgcttcttcg
     9721 cccgcgtgat ggatcgctac gaccgctcgc tcgtgttcgt catcgatcac caggccgcca
     9781 cgctggtcgt ggccgtcgcc acgctcgtcg tcaccgcgct gctgtacgtc gtcatcccga
     9841 agggtctctt tccgacgcag gacaccggtc agctcaaggc gcgcatccag gcggcgcagg
     9901 atgtctcgta cgcgcggatg gccgacgtgc aggcgcaggc ggcccgcgca atcctcgccg
     9961 accctgcagt cgccaacctg agctcgatcg tcggcgtcga cgccgcgaac aacacgatgc
    10021 tctcgaccgg cagcatgctg atcaacctga agccgtcgca cggcgaccag caggccgtga
    10081 tgaatcgcct gcgcgagcgg gttcgcgcgg tgccgggcgt cacgctgtac ctgcagccga
    10141 cgcaggacct gacgatcgac gccgagaccg ggccgaccga gtatcgcgcc tcgctggagg
    10201 gcgtcgacac ggcgcagatc acgcgctgga tgcagctttt gatcgcgagg ctgcaggacg
    10261 cgcccgaggt gcgcaacgtg agcagcgacg ccggcgcgca gggtgcgacg actcacattg
    10321 ccgtcgatcg cgataccgca tcgcgcctcg gcatcaccgc gagcgcggtc gacgacgcgc
    10381 tgtacagcgc cttcgggcag cggatcgtgt cgacgatctt caccgacacc aaccagtacc
    10441 gcgtcattct cgaagcgaag cccgggctcg caacgagcgc cgaaggcgtc ggacgcctca
    10501 acctgatctc gtcgagcggc aagccgacgc cgctgtcggc gttcgcgaag atcaccgagc
    10561 agcgcgcacc gctgcagatc acgcacgtcg gccagtatcc ggcgagcacg ctcaacttcg
    10621 acacggcgcc cggcgtggcg ctcgggcacg cggtcgacgc gatccgcacc gcggcgcacg
    10681 acatcgggtt gccgccgcag atcacgatga cgttcctcgg cgcgtcaggc gcgtacgagc
    10741 gctcactcgc aaaccagctg tggctgatcc ttgccgcggt cgtgtgcgtc tacatcgtgc
    10801 tcggcgtgct ctacgaaagc tacgtgcatc cgctgacgat cctgtcgacc ttgccgtctg
    10861 ccggcgtcgg tgcgctgctc gcgctgatca tcaccggcaa cgacctcggc gtcatcagca
    10921 tcatcggcat catcctgttg atcggcatcg tcaagaagaa cgcgatcatg atgatcgact
    10981 tcgcgatcga cgccgagcgc gagcagggca tgagcgcacg cgatgcgatc caccaagcgg
    11041 cgctgttgcg gctgcggccg atcctgatga cgacccttgc cgcattgttc gccgccgtgc
    11101 cgctgatgct gtcgtggggg gtgggcgccg agctgcgccg cccgctcggc ctcgccattt
    11161 tcggcggcct catcgtgagc cagctcttga cgctgttcac cacaccggtg atctacctcg
    11221 cgttcgaccg gctgggcggc gggagcacgc gccggaagat ggcagacgag atgctggccg
    11281 gagcggccac gagcggcgaa gggccgtccc gagccgcgag ttccgccccc gcggggggca
    11341 gcgcagcggc gaagccgcaa gcgtgggggg acgtataaat ctttcgcgtc ccttcgtgtt
    11401 gcggcccatc ggcaccgtgc tgctgaccgc cggcgtcgcg ctcgccggca tcgcgggctt
    11461 cttcgtgctg cccgtcgcgt cgctgccgca gatcgacctg ccggtgatct cggtcagcgc
    11521 gaacattccc ggcgcgagcc ccgagacgat ggcgcaaagc gtcgcgacgc cgctcgagcg
    11581 ccacctcggc acgatcccga gcgtcaacga gatcacgtcg acgagctcgg tcggtgcgac
    11641 gcgggtgacg ctgcagttcg acctcggccg cgacatcgac ggtgccgcgc gcgacgtgca
    11701 ggcggcgatc aacgcgtcgc gcgtc
//
LOCUS       DASSVX010000025         5148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1184112, whole genome shotgun sequence.
ACCESSION   DASSVX010000025 DASSVX010000000
VERSION     DASSVX010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184112"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /gene="gnd"
                     /locus_tag="VFO53_01190"
     CDS             <1..856
                     /gene="gnd"
                     /locus_tag="VFO53_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET8874916.1"
                     /translation="RLSKPRVVWLMVPAAVVDEELALLTPLLEAGDIVIDGGNSYYRD
                     DLRRGNALQSRGIHYVDVGTSGGVAGLVRGYCLMIGGEAAIVAQLDPIFATLAPGGEA
                     VPRAAVDATPGTAERGYLHCGAHGAGHFVKMVHNGIEYGLMAAYAEGLNILRNANAGT
                     RPRASDAETTPMRHPEDYQYELDLPRIAEVWRHGSVISSWLLDLTAAALADDGALANY
                     AGRVSDSGEGRWTIAAAIDEGVPAPVLSAALYQRFSSRGNADFADRVLSAMRHAFGGH
                     VEKPVEPR"
     gene            853..2256
                     /gene="zwf"
                     /locus_tag="VFO53_01195"
     CDS             853..2256
                     /gene="zwf"
                     /locus_tag="VFO53_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET8874917.1"
                     /translation="MTGTTTPVPCSDALVFFGATGDLARKQIFPALQSMIQHGMLDLP
                     IIGIARSAENVDELRAHVRASLEEHGGVDEAAFGKLSARLQYIRGDYGDASTFERLRQ
                     ALGGATRPLHYLAIPPSMFATVVDALAASGCAKDARVMVEKPFGRDLASAEDLNRRLQ
                     RVFPESAIFRIDHYLAKEAVENLLYFRFANAFLEPIWNRNYVASVQITMAERFGVQGR
                     GRFYDEVGAIRDVVQNHMLQVMALLSMDAPVGSGSDAGRDEKLRALRAMRPLDPGDVV
                     RGQFAGYRNEPGVASDSQVETFAAVRLHLDTWRWAGVPFCIRAGKRLPETTTEITVRL
                     KQPPQRVFADTGHGAPNYLRFRLSPDVSISLGARVKLPGTVMAGQQTELVVHRDPCGD
                     TMPYARLLHDAIHGDASLFTSAAAVEAAWRIVDPALGNATPLEPYEPNSWGPADAARL
                     IAGQGGWHDPAPEPGAA"
     gene            2253..2960
                     /locus_tag="VFO53_01200"
     CDS             2253..2960
                     /locus_tag="VFO53_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET8874918.1"
                     /translation="MTSPRDIVFLVDCDNTLLDNDRVQADLRDHLAREFGADSRDRYW
                     AIFEALRSELGYADYLGALQRYRLGDMNDPRLLLMSSYLVDYPFADRLYEGALAALAH
                     LRRLGAAVILTDGDVVFQPRKVQRSGLWDAVDGHVLIYIHKEQMLDAVAQDYPARHYV
                     MIDDKLRVLAAMKDAWGDRLTTVFARQGHYAFDAANIAAYPPAELSVDRIGDLVNHER
                     AAFLGSRDAECMEQEQR"
     gene            2957..4054
                     /gene="tal"
                     /locus_tag="VFO53_01205"
     CDS             2957..4054
                     /gene="tal"
                     /locus_tag="VFO53_01205"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET8874919.1"
                     /translation="MKATQRLHELGQSLWLDNITREMLVNGTLRRYIDDFEITGLTSN
                     PTIFDHAIRNGSFYDDSIRRKTAAGQSGEDLFIDLALEDLTQAADLFGPVHEATGGVD
                     GWVSMEVSPLLADDTAGSIREAKRLRARARRANLFIKIPGTPAGVPAIEEAIFAGVPL
                     NVTLLFSREQYLAAANAYLRGIERRIAAGLDPRVDSAASLFVSRWDVAVKDKVPRELR
                     NRLGIAIAKRTYKAYRELLASPRWRALAGAGARVQRLLWASTGTKDPEAPDTLYIEAL
                     AAPDTINTIPDKTLLAFADHGQVEGVMPVDGGDAEQVIDEFRRAGVDDAAVAAQLQVE
                     GTQAFDKSWRDLLDCIAAKSEALEKTAPARG"
     gene            4134..>5148
                     /gene="pgi"
                     /locus_tag="VFO53_01210"
     CDS             4134..>5148
                     /gene="pgi"
                     /locus_tag="VFO53_01210"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET8874920.1"
                     /translation="MRPAGLTRRPAWKALQTHCETVRHLHLRRLFADDPRRGERLAAE
                     AAGLYLDYSKNRITDETLQLLLALAGECGLRARIDAMFSGEKINVTEQRAVLHVALRA
                     PRGERIEVDGTDVVPGVHAVLDRMAAFADQIRSGQWLGHTGKRIRNVVNIGLGGSDLG
                     PVMAYQALRHYSHRDLRFRFVSNVDPTDFVEATLDLNPEETLFIISSKTFTTLETVTN
                     AHAARAWALRGLGDERAVSRHFVAVSTNAAEVSKFGISPGNMFGFWDWVGGRYSMDSA
                     IGLSTMIAIGPEQFRAMLAGFHAMDEHFRTAPFDRNLPVLMALLGIWYADFFGAETVA
                     VLPY"
BASE COUNT          871 a         1707 c         1702 g          868 t
ORIGIN      
        1 acgcctttcc aagccgcgcg tggtctggtt gatggtgccg gcggcggtcg tcgacgagga
       61 gctcgcgctg ctcaccccgc tgctcgaagc cggcgacatc gtcatcgacg gcggcaactc
      121 gtattaccgt gacgatctgc gccgcgggaa cgccttgcaa tcccgcggca tccattacgt
      181 tgatgtcggg acgagcggcg gcgtcgcggg tctcgtgcgc ggctattgcc tgatgatcgg
      241 cggcgaagcc gccatcgtcg cgcagctcga cccgattttc gccacgctgg cgccgggcgg
      301 cgaagcggta ccgcgcgccg cggtcgacgc gacaccggga acggcggagc gcggttatct
      361 gcattgcgga gcgcacggcg cgggtcactt cgtcaagatg gtgcacaacg gcatcgagta
      421 cggcctgatg gcggcgtacg ccgaggggct caacatcctg cgcaacgcca atgcgggaac
      481 gcggccgcgc gcgagcgatg ccgaaacgac gccgatgcgc catcccgagg actaccaata
      541 cgagctcgac ctgccgcgga tcgccgaagt atggcgacac ggcagcgtca tcagctcgtg
      601 gttgctcgat ctcaccgctg cagcacttgc ggatgacggc gcactggcga actacgcggg
      661 gcgcgtgtcc gattcgggtg aagggcgatg gacgatcgcg gcggcgatcg acgagggcgt
      721 gccggccccg gtgctgagtg ccgcgctcta ccagcgcttc agctcgcgcg ggaacgccga
      781 cttcgccgat cgcgtgctgt cggcgatgcg ccacgcgttc ggcggacacg tggaaaagcc
      841 ggttgagccc cgatgacggg cacgacgaca ccggtgccgt gctcggatgc gctggtcttt
      901 ttcggcgcca ccggcgacct tgcgcgcaag cagatctttc ctgcgttgca gtcgatgatc
      961 cagcatggaa tgctcgacct gccgatcatc ggcatcgcgc gctcggcgga gaacgtcgat
     1021 gagcttcgcg cgcacgtgcg ggcaagcctc gaggagcacg gcggcgtcga cgaggcggca
     1081 ttcggcaagc tctccgcccg actccagtac atccgcggcg attatggcga tgcgtcgacc
     1141 ttcgaacggc tgcgccaggc gctcggcggc gccacgcggc cgctgcatta cctcgcgatt
     1201 ccgccgagca tgtttgccac ggtcgtcgac gcattggccg cgtcggggtg tgcgaaggac
     1261 gctcgagtga tggtggaaaa accgttcggc cgcgatctcg cgtccgcgga ggacctcaat
     1321 cgccggctgc agcgcgtctt tcccgaatcc gcgatcttcc gcatcgacca ttacctcgcg
     1381 aaggaagcgg tcgagaacct gctctatttc cgcttcgcca acgcattcct cgagccgatc
     1441 tggaatcgca attacgtcgc gagcgtgcag atcacgatgg cggagcgctt cggggtgcag
     1501 ggacgcggcc gattctacga cgaggtcggc gcgatccgcg acgtcgtgca gaaccacatg
     1561 ctgcaggtga tggcattgct ctcgatggat gcgccggtgg gatccggttc cgacgccggc
     1621 cgcgatgaaa agctgcgcgc gttgcgtgca atgcggccgc tcgaccccgg cgacgtggtg
     1681 cgcggacagt tcgccggcta ccgcaacgag cccggggtcg cgtccgattc gcaggtcgaa
     1741 acctttgcgg ccgtgcgcct tcatctcgac acgtggcgct gggccggcgt gccgttctgc
     1801 atccgtgcgg gcaagcgact cccggagacg accaccgaaa tcaccgtaag gctgaagcag
     1861 cctccgcaaa gggtcttcgc ggacaccgga cacggcgcgc cgaattacct ccgcttccgg
     1921 ttgagccccg atgtatccat ttcattggga gcgcgcgtca agctgcccgg aaccgtcatg
     1981 gccggacagc agaccgagct cgtcgtgcat cgcgatccgt gcggagacac gatgccctac
     2041 gcgcgactgc ttcacgatgc gattcacggc gatgcgtcgc tgttcacgag tgccgcagcc
     2101 gtggaagcgg cgtggcgcat cgtcgacccg gcgttgggca acgccacgcc gctcgagccc
     2161 tacgaaccga atagctgggg cccggccgat gctgcccggc tgattgcggg ccagggcggc
     2221 tggcacgatc cggcaccgga gccaggggcc gcatgacgtc gccgcgcgac atcgtgtttc
     2281 tcgtcgattg cgacaacacg ctgctcgaca acgatcgcgt gcaagctgat cttcgtgacc
     2341 atctcgcgcg cgagttcggc gccgacagcc gggatcgtta ctgggcgatc ttcgaggcgc
     2401 tgcgctccga gctcggctac gccgattatc tcggtgcgtt gcagcgctac cggctcggcg
     2461 acatgaacga tccgcggctc ctgctgatgt cgtcctacct cgttgattat ccgttcgccg
     2521 atcgcttgta cgaaggggcg ctcgccgcgc tcgcgcatct gcggcgcctt ggagctgcag
     2581 tcatccttac ggacggcgac gtcgtctttc agccgcgcaa ggtacagcgt tccgggctgt
     2641 gggacgcggt cgacgggcac gtgctgatct acatccacaa ggaacagatg ctcgacgccg
     2701 tcgcgcagga ctacccggcg cgccactacg tcatgatcga cgacaagctg cgcgtcctcg
     2761 cggcgatgaa agacgcatgg ggcgatcgcc tgacgacggt tttcgcccgc caggggcatt
     2821 atgcgttcga tgccgccaac atcgccgcct atccaccggc cgaattgtcg gtcgatcgca
     2881 ttggcgatct cgtcaaccat gagcgcgcgg cattcctcgg cagccgcgat gccgaatgca
     2941 tggaacagga gcagcgatga aagcgacaca acgactgcac gagctcgggc aaagcctctg
     3001 gctcgacaac atcacgcgcg agatgctcgt caacggcacg ctgcggcgct acatcgacga
     3061 tttcgagatc acggggctca cttccaaccc gacgattttc gatcacgcca ttcgtaacgg
     3121 cagcttctac gacgattcga ttcgccgcaa gacggcggcg ggccagtcgg gcgaggacct
     3181 gttcatcgat ctggcactcg aggacctgac gcaggcggcg gacctgttcg gtccggtgca
     3241 cgaggcgacc ggcggcgtcg atggctgggt gtcgatggaa gtgtcgccgt tgctggcgga
     3301 cgatacggcg ggcagcatcc gcgaagcgaa gcggctgcgc gcgcgagcgc gacgcgccaa
     3361 cctcttcatc aagatccccg gaacacctgc gggcgtcccg gcgatcgagg aggccatatt
     3421 cgccggtgtg ccgctcaacg tcacgctgct gttctcgcgc gaacagtacc tggccgcggc
     3481 gaacgcctac ctgcgcggca tcgagcggcg gatcgccgcg ggcctcgatc ccagggtcga
     3541 ctcggcagcg tcgctgttcg tgagccggtg ggacgtcgcg gtcaaggaca aggtgccgcg
     3601 cgagcttcgc aatcgcctgg ggatcgccat tgccaagcgc acctacaagg cttatcgcga
     3661 actgctggcg tcgcctcgat ggcgcgcatt ggcaggtgcg ggcgcccgcg tgcaacgcct
     3721 gctctgggcc agcaccggca ccaaggatcc cgaggcgccc gatacgctgt acatcgaggc
     3781 gctcgccgcc ccggacacga tcaacacgat ccccgacaag acgttgctcg catttgccga
     3841 tcacggtcag gtggaaggcg tgatgcccgt cgacgggggc gatgccgaac aggtcatcga
     3901 cgaattccgg cgcgccgggg tggacgacgc ggccgtggcc gctcagcttc aggtcgaagg
     3961 cacgcaggcg ttcgacaagt cgtggcgcga cctgctcgac tgcatcgcgg cgaaaagcga
     4021 agcgctcgaa aagaccgcgc ctgcccgagg atgaccgcga tgcactgcgc actgcggcgc
     4081 gacgcccggg cgccaatcac tgcacgggga aatcatcgat ggaaaatccc gaaatgcgcc
     4141 cggcagggct gacgcgacgc ccggcgtgga aagcgttgca gacgcattgt gagacggtgc
     4201 gccatctcca cctgcggcgg cttttcgccg acgatccccg gcgcggcgag cggctcgctg
     4261 ccgaagcggc cggcctctac ctcgattact ccaagaaccg cattaccgac gaaaccctgc
     4321 aactgctgct ggcgcttgcc ggcgagtgcg gcctgcgcgc gcgcatcgat gcgatgttca
     4381 gcggcgaaaa gatcaatgtg accgagcagc gcgcggtcct ccatgtcgcc ttgcgtgcgc
     4441 cgaggggcga acggatcgaa gtcgacggca ccgatgtcgt gccgggcgta cacgccgtgc
     4501 tcgatcggat ggcggcgttc gccgaccaga tccgcagcgg tcaatggctc ggacataccg
     4561 gcaagcgcat tcgcaacgtc gtcaacatcg gcctcggggg ctccgacctc ggcccggtca
     4621 tggcgtatca ggcgttgcgc cattacagcc accgcgatct tcggtttcgc ttcgtctcga
     4681 acgtcgaccc caccgatttc gtggaggcga cgctcgacct caatcccgaa gaaacgctgt
     4741 tcatcatctc gtcgaagacg ttcacgacgc tcgaaacggt gacgaatgcc catgccgctc
     4801 gtgcgtgggc cttgcgcggg ctcggcgacg agcgcgccgt cagccggcac ttcgtcgccg
     4861 tgtcgaccaa cgcggcggag gtgtcgaaat tcggcatcag tcccggcaac atgttcggct
     4921 tctgggactg ggtcggcggc cgctattcga tggactccgc gatcggcctg tcgacgatga
     4981 tcgccatcgg tccggaacag ttccgggcca tgctcgccgg gttccacgcc atggacgagc
     5041 acttccggac cgctcccttc gatcgcaacc tgccagtctt gatggcgctc ctcggtatct
     5101 ggtacgccga cttcttcggc gccgagaccg tcgccgtcct tccctacg
//
LOCUS       DASSVX010000026        14584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1517469, whole genome shotgun sequence.
ACCESSION   DASSVX010000026 DASSVX010000000
VERSION     DASSVX010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 14584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14584
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517469"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1203
                     /locus_tag="VFO53_01215"
     CDS             13..1203
                     /locus_tag="VFO53_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012337245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HET8874921.1"
                     /translation="MANPREVVIVNGARTAIGDYGGSLKDIPPTELGARVVREAVKRA
                     NVDPGKIGQCVFGNVIHTEARDMYLSRVVAVNGGLPHESGALTVNRLCGSGLQAIVSA
                     AQNVMLGDTDAAVGGGAESMSRGAYANTTQRWGARMNDAKLVDMMVGALTDPFDTVHM
                     GITAENVATKCGITREQQDAFAVESHRRAAKAASEGRFKSQILPIELKTRKGSVQFDT
                     DEHVRADASAEGMAKLRAVFAKENGTVTAGNASGINDAAAAVVLMEMQAAERAGVKPM
                     ARFVGYGHAGIDPKIMGLGPVNAVKAVLERTGLKIRDIDVIESNEAFAAQAMGVSREL
                     DLDPAKVNPNGGAVALGHPIGATGAILTIKAMYELHRSGGRYGLITMCIGGGQGIAAI
                     IERM"
     gene            1205..1825
                     /locus_tag="VFO53_01220"
     CDS             1205..1825
                     /locus_tag="VFO53_01220"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET8874922.1"
                     /translation="MRDIPLFIVALTISAYWLRVGAMVVRARRRQHRDVGVIPERAAE
                     RAMWLVLVPLVLAWCALPWLALGRAQGWRSVPSFALQGAYAPLRWVFALVAVACLVLT
                     IDCWRRMGRNWRMDISDRNTALVTEGLFARIRHPIYAFSIAMMVATAIVLPSVAMLVV
                     AVVHIVLMNIKARNEEAHLARLHGEAYARYVERTGRFFPGPAARNS"
     gene            1842..2798
                     /locus_tag="VFO53_01225"
     CDS             1842..2798
                     /locus_tag="VFO53_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874923.1"
                     /translation="MALTGLSLLALVLATEVMAAPPPLDAALAARIRELDCANVTAAG
                     VRDVLSRAPAPRIVLLQGSVPLVTMAPFARFLIAMGSPEARLVNPRDGQFSYSSFADS
                     AARAGELAFDYEQTGLAPMLIGHSQGGMLAIRTLYELAGAFHDALHVVDPSTGDALSR
                     TSIVDPRTGRSRPVVGLHVSYAAALATGWLPRLLLGQWSMLSRLRKIPDSVDEFTGFI
                     LPHDAIAGNLLGDAPYEAAGQARVRTVVLPESYHHLTLPQVDHLAADPRLHAWIDAWS
                     PGAAAASPAGDTSNLVHATDIWYSVKRHWCLQAQGLLSVPPS"
     gene            2987..4270
                     /locus_tag="VFO53_01230"
     CDS             2987..4270
                     /locus_tag="VFO53_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874924.1"
                     /translation="MTSWPGRLNLFQATMLDWRELHPYNAVHAVRIAQKLDEAALARA
                     IDDVLSGAGLTGLELDRDRCRYAWRGGEAQVAIEAVDPGSDWQASLAHAFERHLNAPF
                     PREGAIEPFRFFAMPVDDGFFVGIAYDHFIAGGDSIVVLLDAIAARHGAKPASAAPLR
                     RYPRTHVHLFARHPVRFARGLARLPALARSCRRTIRPRYRAIEDGHNGFMLFWLDDAQ
                     YLALRATSKRWGVTLNDALMALLLMAQDAAMPGRYTSARRHELAVASIINLRREHRED
                     PRATFGQFLSSFRVAHPVPPGTTLEALALDVHRATTRVKREKLFLTTLFAMAVDRIIG
                     RMQTPKQRMGTYAKSYPVGAGVSSLNVDALWQSGAAPQYVRGVPTGPLSPVVVAVTTS
                     GTRMCMGISYRTTAVTPEEAAALGNDLVSRIDALK"
     gene            4267..5382
                     /locus_tag="VFO53_01235"
     CDS             4267..5382
                     /locus_tag="VFO53_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874925.1"
                     /translation="MTLTSLRWLAASLVAVSVALAGCATQPLEQSAAAAAHPATPAAP
                     LVPDRVLQERLLALDPEHISDADVQQVLVYGPTPRIMLLYGGIYPVKPIMQSFGYFLV
                     RMGYPEARIRDPGDQDWSYSPYDDAAKLAGIVAWDYERTGVRPMLIGHSQGGMQAVKV
                     LHELAGHFDDSLRVYDPLTGAFEERTTIVDPLTHRTRPVVGVSASYASAVGAGGATYL
                     MPNQWMMVDKLQSIPNTVDEFTGFAIPIDILGGDSHYTQNGTATVRNVDLPVNYSHVF
                     VPAAGALPENPDVRHWINAYVPGRKHDTSSLPIEAAQHVLWAADVWYSIKKHWCLEAQ
                     RLVRAERTRAPTQNAERIRAPDERASRTDAPTESASR"
     gene            5515..6270
                     /gene="fabL"
                     /locus_tag="VFO53_01240"
     CDS             5515..6270
                     /gene="fabL"
                     /locus_tag="VFO53_01240"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HET8874926.1"
                     /translation="MTRALQGQVALVTGAARGIGRAIARKLGAEGADVAVNYYNSHDE
                     AETLCAELRALGRRAYAIQGSVGVPDSVDDMFAALREHFDRVDIVISNAASGVLKPVM
                     EMRLKHWRWCMETNALAVDLLAQRAVPMMRDGGRIIAMSSLGAQRAMPGYGFIGASKA
                     ALEALVRALAQELGPRGIRVNTVSAGVVDTDALAYFPNRDELLANFAQRTPAGPVLRP
                     EDVANAVYLLCLPEAAMINGHTLVVDGGFCISG"
     gene            6419..7171
                     /locus_tag="VFO53_01245"
     CDS             6419..7171
                     /locus_tag="VFO53_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8874927.1"
                     /translation="MKPSGLAGKSVLVTGGSRGIGRAVVETFAGEGAAVTFFYRDNAA
                     AADSVVATLRDAGHDVHAERVDVTGAAACIAAVDILAERNGRIDVLVNNAGVIRDNPL
                     TGLSDDDIETVLDTNVGGVFNVTRAVVPYMVMQRAGCIVNLSSVAGDKGGRGQTNYAA
                     SKGAVNALTRALAVELAPRNIRVNAVAPGVIDTEMSKNVRDMAGDEAKARILMKRYGT
                     AQDVANAVWFLASDYASYVTGQVFHVDGGFKL"
     gene            7196..7612
                     /locus_tag="VFO53_01250"
     CDS             7196..7612
                     /locus_tag="VFO53_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET8874928.1"
                     /translation="MAEIAVTQDEIMAVYPKVAETMADALGCDVEDIEPDVSLIEGLD
                     AESIDFLDMVFRLERAFKVKIPRGKIVENARGDLPESEFEQNGIVTEKGLAQLRDYLS
                     EVPADRFKTPMKVADIPRLFTPTTFCKLVVRAQKEA"
     gene            7718..8551
                     /locus_tag="VFO53_01255"
     CDS             7718..8551
                     /locus_tag="VFO53_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874929.1"
                     /translation="MSDAHFAAYSFVDRIVELVRGKRARGVFAIPARIAAFPTALVAE
                     AVGQLAAWVAMDAIGFRGRPVAALATETRFLHDAKPGQRLELAVDIQECDDDAVAYGG
                     YASVDGRRIIELVDCLGPMLPLADFDAPEALRERLTLLRGEGAPPDRFAGVGDIPVTV
                     THHVPGHELAASLAVPAAAPFFADHFPRRAVFPATLLLDLMMRLALDAARGSDALGRR
                     PQALRITHVKMRSFIEPSQHLDIGVTLDSPEDGVARAKLTAKTGERLVASARLELAAG
                     D"
     gene            8554..9858
                     /locus_tag="VFO53_01260"
     CDS             8554..9858
                     /locus_tag="VFO53_01260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET8874930.1"
                     /translation="MHQPRRRVAITGIGLVTPVGNDVAATWASLDAVRNGAAPITLFD
                     ASGFSTRIAAEVKHFDDEAITAPRRLLKFANRSHRFALAAAEEAMRDAGIAPSGDDAQ
                     RWGCAVGTGMMGVAYSDLSEVQRHSAPDGTIDCRKLLTDPSAADPLAFCRSQSTAGVA
                     LLTRRFGIRGSATSVHTACASGGQAIGTALKLIRRGAVDRALAGGFDSMISPVGLAGF
                     CLLSAVSTDNETPQRASRPFDATRNGFVLGEGAGFLVLEEWDAAKRRGARVYAELAGD
                     GNSLSSYRITDSPPNGDGPIQSMRAALADARATPADIDYINAHGTSTGMNDRSETAAI
                     HAVFGDDAKRVFVSSTKSVMGHLIAAAGAVEVAICALAIDRGEMPVNANYRESDPDCD
                     LNLVLERPRRQKLRMTLSNSFGFGGSNSCVALRSPDLVAEEP"
     gene            9855..11132
                     /locus_tag="VFO53_01265"
     CDS             9855..11132
                     /locus_tag="VFO53_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET8874931.1"
                     /translation="MRSRVVVTGMGAVCGAGRTPEAMLAAIVAGRSAIGPIRGFDTTG
                     WAVTNAAEIADFNARALVDDRKLHKLIRRTDMVGIYAGDRAAEAAGYAAYRDALDDDA
                     ANAFCDRTGVYVGSGAGAFENQYDFFPLMAEANGDLVKFGQHLPDTVNPMWLLRTLPN
                     NVLCHVGIRNRLKGANACITNHSVGGTLAVIEAAEALRHGEADRALAIGHDTPIEPQN
                     ILYYDQCGLLSHSALRPFDARRDGSLFGEGAGALALETDAAAKARGATIVGEMLGGGY
                     VSEASGLLAIRDDGDGVARAVEAALADAGIDAADVGMIVAHGNGTSLSDASEAAGLHR
                     VFGDRCPPVTAFKWAIGHLIAAAGIVETTIALAALRARSVPGIATYGQPDPACAALPV
                     SGEAQKPRSDVALVLCRGFASTDAALLVRATPA"
     gene            11129..12403
                     /locus_tag="VFO53_01270"
     CDS             11129..12403
                     /locus_tag="VFO53_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HET8874932.1"
                     /translation="MTPSPLPAPPETPVRCGIDSVELARIERLLEQTPAADLAKIFSA
                     QELLDSGAGPGRVASLAARYAAKEACVKLFPRELSAGRIEPADFAVTRDGFGAPHIVA
                     SAGARAIMDRARVGDIAVSLTHDRTSASAVALALRLETTAPMAGRVLFRVLPIRRKVI
                     VDNLRRVFGSAVPAGEIDRLAQAHYAHLWKLGAEFIRFRWLSRERKRALVRVDGLDVF
                     IDAFRRGKGVLVLTGHFGNWEVATIAGIQNYPEVRGRFHFVRRPIKPRWLDGLVTRRF
                     NAAGFGVVAKRGSLDRIVSLLEQGDVVVFPFDQYAGGADGVDVEFFGEPAGTFKSLAV
                     IALATGAPVLPAACWRQPDGTHVLKFEAPVPPVDVENVNEAIVRTTRAYNAALERLVV
                     RHPEQWWWVHRRFRRARRKPRNRRSAMAAGND"
     gene            12464..13381
                     /locus_tag="VFO53_01275"
     CDS             12464..13381
                     /locus_tag="VFO53_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HET8874933.1"
                     /translation="MLATLLAPRFIVLYLFIASAVYIHYRGRIRHGFFRQLTDHSSLM
                     APYNALMYLFSAVPNRPYVDVGRFPELAPLSANWQAIRAEALQLFGEGRIRAAATYND
                     LGFNSFFKTGWKRFYVKWYDEPLPSAKALCPRTVELVQSIPGVNAAMFALLPPGSKLG
                     AHRDPFAGSLRYHLGLVTPNADTCRIVVDGESYFWRDGEPVMFDETFIHTAENRSDVE
                     RIILFCDVERPLTNPLVRAINRFVKMTLIRASQTENAPGDRVGVLNHVFNAAYRVRLV
                     GKAIKKRSRVAHYTLKWLILGGVLYLIFR"
     gene            13412..13921
                     /locus_tag="VFO53_01280"
     CDS             13412..13921
                     /locus_tag="VFO53_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HET8874934.1"
                     /translation="MSMSLYQASVPVFVKTLTNLKAVLQKAKAHALEHKIEEAAFVNA
                     RLYPDMLPLSRQVQIASDIARGCAARLAGVEPPPYEDKEQSFDELVARIDRTIDYMNG
                     LDQKQFEGAATRTITRPLRGEPHAFTGANYLLQFGTPNVYFHTATAYDILRHNGVPLG
                     KADYLGTLD"
     gene            14074..>14584
                     /locus_tag="VFO53_01285"
     CDS             14074..>14584
                     /locus_tag="VFO53_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874935.1"
                     /translation="MARSAFERAQLRTSLASAPASRRRAMNAIRKSVLYLPGSALLRE
                     CFEETSMNRLCHRFAIGTMLTAFAAIAVAAPPKKSYNLEFLLPDNDELQATSPTNPAY
                     DSTTGQLIPPVAVTVTVKNESPPSTANSNISSFQFTVSGLALLNGSGDIACPNARCSV
                     DPSTGTVYVT"
BASE COUNT         2332 a         5061 c         4891 g         2300 t
ORIGIN      
        1 gaggaggcag ccatggccaa tccacgggaa gtcgtcatcg tcaacggtgc gcgcactgcg
       61 atcggtgatt acggcggttc gctgaaggac attccgccga cggaacttgg cgcccgcgtc
      121 gtgcgcgagg ccgtcaagcg cgcgaacgtc gatccgggca agatcggcca gtgcgtattc
      181 ggcaacgtga tccacaccga agcgcgcgac atgtacctgt cgcgggtcgt cgcggtcaac
      241 ggcgggctgc cgcacgaaag cggcgcgctt acggtgaacc gcctgtgcgg cagcggcctg
      301 caggcgatcg tgagcgccgc gcagaacgtg atgctgggcg acaccgatgc ggccgtcggc
      361 ggcggcgccg aaagcatgag ccgcggcgca tatgcgaaca ccacgcagcg ctggggcgcg
      421 cggatgaacg atgcgaagct cgtcgacatg atggtcggcg cgctcaccga tccgttcgac
      481 accgtgcaca tgggaatcac cgccgagaac gtcgcgacga aatgcggcat cacgcgcgag
      541 cagcaggacg cgttcgccgt cgaaagccat cggcgcgccg cgaaggcggc gagcgagggc
      601 cgtttcaagt cgcagatcct gccgatcgag ttgaagacca ggaaaggcag cgtgcagttc
      661 gacaccgacg agcacgtccg cgccgacgcg agcgccgaag gcatggccaa gctccgcgcg
      721 gtcttcgcga aggaaaacgg cacggtgacg gcgggtaacg cgtcgggcat caacgatgcc
      781 gcggcggcgg tcgtgctgat ggaaatgcag gcggccgagc gcgccggcgt gaagccgatg
      841 gcgcgtttcg tcggctacgg ccatgcgggc atcgatccga agatcatggg cctcggtccc
      901 gtcaacgccg tcaaggcggt cctcgagcga accgggctca agatccgcga catcgacgtc
      961 atcgagtcga acgaagcgtt cgcggcgcag gcgatgggcg tgtcgagaga gctcgacctc
     1021 gatcctgcga aggtcaaccc gaacggcggc gccgtcgcgc tcggccaccc gatcggcgcc
     1081 accggcgcca tcctcacgat caaggcgatg tacgagctgc atcgcagcgg cggccgctac
     1141 ggcctcatca cgatgtgcat cggcggaggc caggggatcg cggcgatcat cgagcggatg
     1201 tgatttgcgc gatatcccgc ttttcatcgt cgcgctgacg atttccgcgt actggcttcg
     1261 cgtcggcgcc atggtggtgc gcgcgcggcg ccggcagcat cgcgacgtgg gcgtcatccc
     1321 cgagcgcgcg gccgagcgcg ccatgtggct cgtgctcgtg ccgctggtgc tcgcctggtg
     1381 cgcgctgccg tggcttgcgc tcggccgcgc gcagggctgg cgttccgtcc cgtcgttcgc
     1441 gctgcaaggc gcctatgcgc cgctgcgctg ggtcttcgcc ctcgtcgccg tcgcctgcct
     1501 cgtcctcacc atcgactgct ggcgcaggat gggccgcaac tggcgcatgg acatcagcga
     1561 ccgcaacacg gcgctggtga ccgagggcct gttcgcgcgc atccgccatc cgatctacgc
     1621 gttcagcatc gcgatgatgg tcgccaccgc gatcgtgctg ccgagtgtcg cgatgctcgt
     1681 cgtcgccgtt gtccatatcg tgctgatgaa catcaaggcg cgcaacgagg aagcgcatct
     1741 cgcccgcctg catggcgagg cctatgcgcg ctacgtcgaa cgcaccgggc ggttcttccc
     1801 aggacccgcg gcgcgaaatt cgtagtccgc ggcgcgtgcg cgtggcgctg accggcctgt
     1861 cgctgctcgc gctggtcctc gcgaccgaag taatggcggc gccgccgccg ctcgacgcgg
     1921 ccctcgccgc ccgcatccgc gagctcgact gcgcgaacgt cacggcggcc ggcgtgcgcg
     1981 acgtgctgtc gcgggcgccc gcgccgcgca tcgtgctgct gcagggcagc gtcccgctcg
     2041 tcacgatggc gccgttcgcg cgcttcctga tcgcgatggg ctctcccgag gcgcgcctcg
     2101 tcaatccgcg cgatgggcag ttttcgtaca gcagcttcgc cgacagcgcc gcccgcgccg
     2161 gcgagctcgc gttcgactac gagcaaaccg gcctggcgcc gatgctgatc ggccacagcc
     2221 agggcggcat gctggcgata cgaacgctgt acgagctcgc cggcgcgttc cacgacgcac
     2281 tgcatgtcgt cgatccttcg acgggcgacg cgttgtcgcg tacgtcgatc gtcgacccgc
     2341 gcaccgggcg gtcgcgcccg gtcgtcggcc tgcacgtcag ctacgccgcg gcgctcgcca
     2401 ccgggtggct gccgcgactg ctgctcggcc agtggtcgat gctgtcacgc ctgcgaaaga
     2461 ttccggacag cgtcgacgaa ttcaccggat tcatccttcc gcacgacgcg atcgccggaa
     2521 acctcttagg cgatgcgccg tacgaggctg ctggccaagc gcgcgtgcgc acggtcgtgc
     2581 tgcccgaaag ctatcatcac ctgacgctgc cgcaggtgga ccatctcgcg gccgacccgc
     2641 gcctccatgc ctggatcgac gcatggtcgc ccggcgcggc ggcggcgtcc ccggccggcg
     2701 acacgtcgaa tctcgttcat gccaccgaca tctggtacag cgtcaagcgc cactggtgct
     2761 tgcaggcgca gggattgctc agtgttcccc catcgtgatg cctttcgcct ccgcagcctg
     2821 cctcgattcg gggctgccct gcgctttccc gcggggcgac cggcgtatgg catggcggga
     2881 accgtatcgg caacgggcgg ttcctggaaa atttttccgt acctcgcgtg aaaaaaaccg
     2941 ggccgatgcg ggcgatacgc ctacaaggcc gcggggggcg cggacgatga cgtcctggcc
     3001 gggccggctc aacctgttcc aggcgacgat gctcgactgg cgcgagcttc atccgtacaa
     3061 cgccgtccac gccgtccgca tcgcgcaaaa gctcgacgag gccgcgctcg cgcgcgccat
     3121 cgacgacgta ttgtcgggcg ccgggttgac cggtctcgag ctcgatcgcg atcgctgccg
     3181 ctacgcctgg cggggcggcg aggcgcaggt cgcgatcgag gcggtcgatc ccggcagcga
     3241 ctggcaggcg tcgctcgcac acgcgttcga gcgccacctc aatgcgccgt ttcctcgcga
     3301 aggcgcgatc gaaccgttcc gctttttcgc catgccggtc gacgacggct tcttcgtggg
     3361 aatcgcctac gatcatttca tcgccggcgg cgactcgatc gtcgtgctgc tcgatgccat
     3421 cgcagcgcgt cacggggcga agcctgcgag tgctgcgccg ctgcggcgct acccgcgcac
     3481 gcacgtgcac ctcttcgcac gccatccagt gcgcttcgcg cgcggcctcg cccggcttcc
     3541 cgcactcgcc cgcagttgca ggcgcacgat ccgcccgcgt tatcgcgcca tcgaggatgg
     3601 ccacaacggc ttcatgctct tctggcttga cgacgcccaa tatctggcgc ttcgtgccac
     3661 gtcgaagcga tggggcgtga cgctgaacga cgcgctgatg gcgctgctgc tcatggcgca
     3721 ggacgccgcg atgccgggac gctacacgtc ggcacgccgc cacgagcttg ccgtcgcgtc
     3781 gatcatcaac ctgcgccgcg agcaccgcga agacccgcgc gcgacgttcg gccagttcct
     3841 gagctcgttt cgcgtcgcgc atccggtgcc gccgggaacg acgctcgagg cactcgcgct
     3901 cgacgtgcat cgcgccacga cgcgcgtcaa gcgcgagaag ctcttcctca cgaccctttt
     3961 cgcgatggcc gtcgaccgga tcatcggccg catgcagacg ccgaagcagc gcatgggaac
     4021 ctacgcgaag agctatccgg tgggcgccgg cgtgtcgtcg ctcaatgtcg acgcactgtg
     4081 gcaaagcggc gccgcgcccc aatatgtccg tggcgtgccc accggcccgc tgtcgccggt
     4141 cgtcgtcgcg gtgacgacgt cgggcacacg catgtgcatg ggcatttctt acaggaccac
     4201 ggcggtgact ccggaggagg ccgcagcact cggcaacgat ctcgtctccc gcatcgacgc
     4261 actcaaatga cactcacttc cctgcgctgg ctcgccgcct cgctcgtcgc cgtttcggtg
     4321 gcgctcgcag gctgcgcgac gcagccgctc gaacaatcgg cggcggccgc cgcgcatccg
     4381 gccacgcctg cggcgccgct cgttcccgat cgcgtgctgc aggaacgcct gctcgcgctc
     4441 gaccccgaac acatcagcga cgccgacgtg cagcaggtgc tggtgtacgg tccgacgccg
     4501 cgcatcatgc tgctgtacgg cggcatctat ccggtcaagc cgatcatgca gtcgttcggc
     4561 tattttctcg tgcgcatggg gtatcccgaa gcgcgcattc gcgatccggg cgaccaagac
     4621 tggtcgtaca gcccctacga cgatgcggcg aagctcgccg gcatcgtcgc atgggattac
     4681 gagcgcaccg gcgtgcggcc gatgctgata ggccacagcc agggcggcat gcaggccgtc
     4741 aaggtgctgc acgaactggc cgggcacttc gacgattcgc tgcgtgtgta cgacccgttg
     4801 acgggagcat tcgaggaacg cacgacgatc gtcgatccgc tcacgcacag gacgcgcccg
     4861 gtcgtcggcg tctcggcgtc gtacgcgtcg gcggtgggcg ccggcggcgc aacgtacctg
     4921 atgcccaacc aatggatgat ggtcgacaag ctgcaatcga ttccgaatac cgtcgacgaa
     4981 ttcacgggat ttgcgatccc catcgacatc ctcggcggcg actcgcacta cacgcaaaac
     5041 ggcacagcca ccgtgcgcaa cgtcgatctt cccgtgaact acagtcacgt gttcgtgccc
     5101 gccgcaggcg cgctgcccga gaacccggac gtgcgtcact ggatcaacgc ttacgtgccg
     5161 gggcgcaagc acgacacgtc gtcgttgccg atcgaggcgg cgcagcacgt gctgtgggcg
     5221 gccgacgtgt ggtacagcat caagaagcac tggtgcctcg aggcgcagcg tctggtccgc
     5281 gccgagcgga cccgtgcgcc gacgcagaac gccgagcgca ttcgcgcccc cgacgaacgc
     5341 gcgtcgcgca ccgacgcgcc cacggaaagc gcttcgcggt aaggcgacgc gagggcgggt
     5401 agaatagcgc ccttttccgc gtgcgccgcg ccccgtgccg ggcacgcgcc gaagcgtggc
     5461 aaatcgccgt cgccgggtgc gcagctcgcg cccgcctcca accgcagtga aacgatgaca
     5521 cgcgccctgc aaggccaagt tgcgctggtg accggcgctg cgcgcggcat cggccgcgcc
     5581 atcgcgcgca agctcggcgc cgaaggcgcc gacgtcgcgg tcaactacta caacagccac
     5641 gacgaagccg agacactgtg cgccgagctt cgtgcgctcg gccggcgcgc gtacgcgatc
     5701 cagggcagcg tcggcgtccc cgacagcgtc gacgacatgt tcgcggcgtt gcgcgagcat
     5761 ttcgaccgcg tcgatatcgt gatcagcaac gccgcgtcag gcgtgctgaa gccggtcatg
     5821 gagatgcgac tcaagcactg gcgctggtgc atggagacga atgcgctcgc ggtggacctc
     5881 ctcgcgcagc gcgccgtgcc gatgatgcgc gacgggggcc ggatcatcgc gatgtcgagc
     5941 ctcggcgcgc agcgggcgat gcccggctac ggcttcatcg gcgcgtcgaa ggccgcgctc
     6001 gaggcgctcg tgcgcgcgct tgcccaggag ctcgggccgc gcggcattcg cgtgaacacg
     6061 gtgagcgcgg gtgtggtcga caccgacgcg ctcgcgtact tccctaaccg cgacgagctg
     6121 ctcgccaatt tcgcgcagcg cacgccggcg ggacccgtgc tgcggccgga agacgtcgcc
     6181 aacgccgtct acctgctgtg cctgcccgag gcggcgatga tcaacgggca cacgctggtc
     6241 gtcgacggcg gcttctgcat ttccggatga tgagcgccgc cgggccgacc cagggcgcga
     6301 attgctcccc cctcgggggc agcgcagcgc gtgagcccga cgggccgccc cgagggcgaa
     6361 cagccgagtg caaagcacga gggacgggta accggccgca agcgtggggg gtacaccgat
     6421 gaagccgtcc ggactcgccg gcaagtcggt gctggtcacg ggcggcagcc gcggcatcgg
     6481 gcgcgcggtc gtcgagacct tcgccggcga aggcgcggcg gtgacgttct tctatcgcga
     6541 caatgccgcg gcggccgaca gcgtcgtcgc gacgctgcgc gatgccggac acgacgtgca
     6601 cgccgagcgc gtggacgtga cgggcgccgc cgcgtgcatc gccgcggtgg acatcctcgc
     6661 cgagcgtaat ggacgcatcg acgtgctcgt gaacaatgcg ggcgtgatcc gcgacaaccc
     6721 gctcaccggc ctgtccgacg acgacatcga aacggtgctc gacacgaatg tcggcggcgt
     6781 gttcaacgtg acgcgcgccg tcgtgccgta catggtgatg cagcgcgccg ggtgcatcgt
     6841 gaacctgagc tcggtcgccg gcgacaaggg cggacgcggg cagacgaact acgccgcaag
     6901 caagggtgcg gtcaacgcac tgacgcgtgc gctcgccgtc gagctcgcgc cccgcaacat
     6961 tcgcgtcaac gccgtcgcac ccggcgtcat cgacaccgaa atgtcgaaga acgtgcgcga
     7021 catggcaggc gacgaggcaa aggcgcgcat cctgatgaag cgctacggga ccgcgcagga
     7081 cgtcgcgaac gccgtctggt ttctcgcgtc cgactacgcg agctacgtga cggggcaggt
     7141 gttccacgtc gacggcggct tcaagctctg aaacctctga agggagaagg cggcaatggc
     7201 agagattgcg gtgacgcagg acgaaatcat ggcggtgtat ccgaaggtgg ccgagacgat
     7261 ggccgacgcg ctcggctgcg acgtcgagga catcgagccc gacgtgtcgc tgatcgaggg
     7321 gctcgacgcc gagtcgatcg acttcctcga catggtgttc cggctcgagc gcgcgttcaa
     7381 ggtcaagatc ccgcgcggca agatcgtcga gaatgcgcgc ggcgacctgc ccgaatcgga
     7441 attcgagcag aacggcatcg tcaccgaaaa gggactcgcg cagctgcgcg actacctgtc
     7501 ggaggttccg gccgatcgct tcaagacgcc gatgaaggtc gccgacatcc cgcggctgtt
     7561 cacaccgacg acgttctgca agctcgtcgt gcgcgcgcag aaggaagcct aggggtcaga
     7621 taccatttat cgcctcggac tttcattaca gcaccgaggc gaaccgataa atggtgtctg
     7681 atcccatttc ggttttgacc ccatttcggt tttctcgatg tccgacgccc atttcgccgc
     7741 ctattcgttc gtcgaccgca tcgtcgagct cgtccggggc aagcgggcgc gcggcgtgtt
     7801 cgccatccct gcgcgcatcg ctgcgtttcc cacggcgctc gtcgccgaag cggtcggcca
     7861 gctcgctgcg tgggtcgcca tggacgccat cggctttcgc ggccggcccg tggcggcgct
     7921 cgcgaccgag acccggttcc tccacgacgc gaaacccggg cagcggctcg agctcgccgt
     7981 cgacatccag gaatgcgacg acgacgccgt tgcctacggc ggctacgcga gcgtcgacgg
     8041 ccggcgcatc atcgagctcg tcgattgcct cggcccgatg cttccgctcg ccgatttcga
     8101 cgcgcccgag gcattgcgcg agcgcctcac gctgctgcgc ggcgaaggcg cgccacccga
     8161 tcgattcgcg ggtgtcggcg acatcccggt gaccgtgacg caccacgtgc caggtcacga
     8221 gctcgccgca agcctcgccg ttcccgcggc cgcgccgttc ttcgccgatc acttcccgcg
     8281 ccgcgccgtg tttcccgcga cgctgctgct ggatctgatg atgcggctcg cgctcgatgc
     8341 ggcgcgcggc tcggatgcac tcggccgacg gccgcaggcg cttcgcatca cgcacgtcaa
     8401 gatgcgctcg ttcatcgagc catcgcagca cctggacatc ggcgtgacac tcgattcacc
     8461 cgaggacggc gtcgcgcggg cgaagctcac cgcgaagacc ggcgagcggc tcgtcgcaag
     8521 cgcacggctc gagctcgcgg cgggggactg acgatgcatc agccacgacg gcgcgttgcg
     8581 atcaccggca tcggactcgt cacgccggtc ggcaacgacg tcgccgcgac gtgggcgtcg
     8641 ctcgacgccg tccgcaacgg cgccgcaccg atcacgctgt tcgacgcgtc gggattttcg
     8701 acccgcatcg ccgccgaagt gaagcatttc gacgacgaag cgatcaccgc gccgcgacgg
     8761 ctcctcaagt tcgccaaccg ctcgcaccgc tttgcgcttg cggccgccga agaggcgatg
     8821 cgcgacgcgg gcatcgcgcc gagcggcgac gacgcgcaac gctggggctg cgcggtgggc
     8881 accgggatga tgggcgtggc gtattcggat ctttccgaag tgcagcgcca ttcggcaccc
     8941 gatggaacga tcgattgccg gaagctgctg accgaccctt ccgcggccga tccgctcgcg
     9001 ttctgccgca gccagagcac ggcgggtgtg gcactcctca cgcgccgctt cggcattcgc
     9061 ggctcggcga cctcggtcca caccgcctgc gcgtcggggg ggcaggcgat cggcaccgcg
     9121 ctgaagctca tacgccgcgg tgctgtggac cgcgcgctgg ccggcggctt cgattcgatg
     9181 atcagcccgg tgggtctcgc gggcttctgc cttctctccg cggtctccac cgacaacgag
     9241 acgccgcagc gcgcgagccg gccgttcgac gcgacgcgca acggtttcgt tctcggcgag
     9301 ggcgcgggct ttctcgtgct cgaggaatgg gacgccgcga agcggcgcgg cgcgcgggtc
     9361 tatgcggagc tcgcgggcga tggcaactcg ctgtcgtcgt accggatcac cgattcgccg
     9421 cccaacggcg acggcccgat ccagtcgatg cgtgccgccc tcgccgatgc gcgcgcgaca
     9481 ccggccgaca tcgactacat caacgcgcac ggcacgtcga ccggcatgaa cgatcgcagc
     9541 gaaaccgccg cgatccacgc ggtattcggc gacgatgcga agcgcgtgtt cgtgagctcg
     9601 acgaagagcg tgatggggca tctgatcgct gcagcgggcg ccgtggaagt cgcgatctgc
     9661 gcgcttgcca tcgaccgcgg cgagatgccg gtcaatgcga actaccgcga atccgatccc
     9721 gactgcgatc tgaacctcgt gctcgaacgg ccgcgccggc agaaactgcg gatgacgctg
     9781 tcgaactcgt tcggcttcgg cggctcgaac agttgcgtcg cgctgcgcag ccccgacctg
     9841 gtcgcggagg aaccgtgaga tcacgcgtcg tggtcacggg catgggcgcg gtttgcgggg
     9901 ccggccgcac accggaagcg atgctcgccg caatcgtcgc gggtcgctcc gccatcggcc
     9961 cgatccgcgg attcgacacg accggctggg cggtgacgaa cgccgccgag attgccgatt
    10021 tcaatgcgcg cgcgctcgtc gacgaccgca agctgcacaa gctgatccgg cgcaccgaca
    10081 tggtgggcat ctatgccggc gaccgcgccg ccgaagccgc gggctacgcg gcgtatcgcg
    10141 atgcgctcga cgacgacgcg gcgaacgcgt tctgcgatcg caccggcgtc tacgtcggct
    10201 ccggcgccgg cgcattcgag aaccagtacg acttcttccc gctgatggcg gaagcgaacg
    10261 gcgatctcgt caagttcggg cagcacctgc ccgacaccgt gaacccgatg tggctgctgc
    10321 gcacgctgcc gaacaacgtg ctgtgccacg tcggcatccg caaccggctg aagggcgcga
    10381 acgcctgcat caccaatcac agcgtcggcg gcacgctcgc ggtcatcgaa gcggcggaag
    10441 cgctgcgcca cggcgaagcc gatcgcgcgc tcgccatcgg gcacgacacg ccgatcgagc
    10501 cgcagaacat cctgtactac gaccagtgcg gcctgctgtc gcacagtgcg ctgcgaccgt
    10561 tcgacgcgcg gcgcgacgga agcctcttcg gcgaaggcgc aggcgcgctg gcactggaaa
    10621 ccgacgcggc ggcgaaggcg cgcggtgcaa cgatcgttgg tgaaatgctc ggcgggggct
    10681 atgtcagcga ggcatcggga ctgctggcga tacgcgacga cggcgacggc gtggcgcgcg
    10741 cggtcgaggc ggcgctcgcc gatgccggca tcgatgcggc cgacgtcggg atgatcgttg
    10801 cacacggcaa cgggacgtcg ctgtccgatg cttcggaagc tgccggtctg caccgcgtgt
    10861 tcggcgaccg ctgtccgccg gtcaccgcct tcaaatgggc gatcggacac ctgatcgccg
    10921 ccgcgggcat cgtggaaacg acgatcgcgc tcgcggcgtt gcgtgcgcgc agcgtgccgg
    10981 gcatcgccac gtacggccag cccgatccgg cctgcgcggc gcttcccgtg tcgggcgagg
    11041 cgcagaagcc gcgcagcgac gtggcgctcg tgctgtgccg gggattcgcg tccaccgatg
    11101 cggcgctgct cgttcgcgca acgcccgctt gacgccgtcc cccctgccgg cgccacccga
    11161 aacgccggtc cgctgcggga tcgacagcgt cgagcttgca cgcatcgagc gcctgctcga
    11221 gcaaacgccc gccgccgatc tcgcgaagat cttctcggcg caagagctgc tggacagcgg
    11281 cgccggaccg ggacgcgtgg cgagcctcgc cgcacgctat gccgccaagg aagcctgcgt
    11341 gaagctcttc ccgcgcgagc tttctgccgg gcgcatcgag cccgccgatt ttgccgtgac
    11401 gcgcgatggt ttcggcgcgc cgcacatcgt tgcgagcgcc ggcgcgcgcg ccatcatgga
    11461 tcgcgcccgc gtcggcgaca tcgccgtttc gctcacccac gaccggacga gcgcgtcggc
    11521 ggtcgcgctc gcattgcggc tggaaacaac ggcgccgatg gccgggcgcg tcctcttccg
    11581 cgtgctgccc atacgccgca aggtgatcgt cgacaacctg cggcgcgtgt tcggcagcgc
    11641 ggtgccggcc ggcgagatcg accgcctggc gcaggcgcat tacgcgcacc tgtggaagct
    11701 cggcgccgag ttcatccgct tccgctggct ttcacgcgag cgcaagcgcg cgctcgtccg
    11761 ggtcgacggg ctcgacgtct tcatcgacgc gtttcgtcgc ggcaagggcg tcctcgtgct
    11821 gaccggtcac ttcggcaact gggaagtggc caccatcgcc ggcatccaga actatccgga
    11881 agtgcgcggg cgctttcatt tcgtgcgccg gccgatcaag ccgcgctggc tcgacggcct
    11941 cgtcacgcgc cggttcaacg cggccggctt cggcgtcgtc gcgaagcgcg gctcgctcga
    12001 ccggatcgtg tcgctgctcg aacagggcga cgtcgtcgtg tttccgttcg accagtacgc
    12061 cggcggcgcc gatggcgtgg acgtcgagtt cttcggcgag cctgccggca cgttcaagag
    12121 cctcgcggtg atcgcgctcg cgaccggcgc gccggtgctc ccggccgcct gctggcgcca
    12181 gccggacggc acgcacgtcc tgaagttcga agcgcccgtt ccaccggtgg acgtcgagaa
    12241 cgtgaacgag gcgatcgtgc gcaccacccg tgcctacaac gcggcgctcg agcggctggt
    12301 cgtccgccat cccgagcaat ggtggtgggt gcaccggcgc ttccggcgcg cgcggcgcaa
    12361 accgcgcaac cgccgcagcg ccatggccgc cgggaacgat taaagtatcg gttctggcat
    12421 tcctgaccgc tcccgcgccc gccgccgacg cccgccaacg cacatgctcg ccacattgct
    12481 tgcgccccgg ttcatcgtcc tgtacctgtt catcgcgtcg gcggtgtaca tccattaccg
    12541 cggacggatt cgccacgggt tcttccgcca gctgaccgac cactcgtcgc tgatggcgcc
    12601 gtacaacgcg ctgatgtacc tcttctcggc ggtgcccaac cggccgtatg tcgatgtcgg
    12661 ccgctttccc gagctcgcgc cgctgtctgc caattggcag gcgatacgcg cggaagcgtt
    12721 gcagctgttc ggcgaaggac gcatccgtgc cgccgccacg tacaacgacc tcggcttcaa
    12781 ttcgttcttc aagaccggct ggaagcgctt ctacgtgaag tggtacgacg agccgctgcc
    12841 gtcggcgaag gcgctttgtc cgcgcacggt cgagctcgtg cagtcgatcc ccggcgtgaa
    12901 cgccgcaatg ttcgcgctgc tgccgccagg cagcaagctc ggcgcgcatc gcgatccgtt
    12961 tgccgggtcg ctgcgctacc acctcgggct cgtcacgccg aacgccgaca cgtgccgcat
    13021 cgtcgtcgac ggcgagtcgt acttctggcg cgacggcgag ccggtgatgt tcgacgagac
    13081 gttcatccac accgccgaga atcgcagcga cgtcgagcgg atcattttgt tctgcgacgt
    13141 cgagcggccg ctcaccaatc cgctcgtccg cgcgatcaac cgcttcgtca agatgacgct
    13201 gatccgcgcg agccagaccg agaatgcgcc cggcgatcgc gtcggcgtgc tcaaccacgt
    13261 cttcaacgcc gcctaccggg tgcgcctcgt cggcaaggcg atcaagaaga ggagccgcgt
    13321 cgcgcactac acgctcaagt ggctgatcct cggcggcgtg ctatacctga tcttccgctg
    13381 atacggcggc tttactccaa ggagtttcgc gatgtcgatg tcgctctacc aggcaagcgt
    13441 accggtgttc gtcaagacgc tgaccaattt gaaagccgtg ctgcaaaagg cgaaggcgca
    13501 cgcgctcgag cacaagatcg aggaggcggc gttcgtcaac gcgcggcttt atcccgacat
    13561 gctgccgctg tcccgccagg tgcagatcgc gagcgacatc gcgcgcggct gcgcggcccg
    13621 gcttgccggc gtcgagccgc cgccgtacga ggacaaggag cagagcttcg acgagctcgt
    13681 ggcgcgcatc gaccggacga tcgactacat gaacggcctc gaccagaagc agttcgaagg
    13741 cgccgcgacg cgcaccatta cccggccgct gcgcggggag ccgcacgcgt tcaccggcgc
    13801 gaactacctg ctgcagttcg gtaccccgaa cgtgtatttc cacacggcga cggcgtacga
    13861 catcctgcgg cacaacggcg tgccgctggg gaaggccgat tacctgggca cgctcgatta
    13921 gcggctgttc agcgcacgtt gagggcgagg ctgcaggcca gtaattcggc cggcgattat
    13981 tccccagtcg aaccgtggaa aattacgtgc ctcgatccgg ggaaagcggc tatacctatc
    14041 ggagtattct tgccgggacc gggtcaacgt agcatggcgc gttcggcgtt cgaacgcgcc
    14101 cagttgcgaa cgtctctcgc gtcagcgccg gcttcgagga ggagggccat gaacgcgatc
    14161 cgcaagtccg tcctgtatct gccaggcagc gcgctcctgc gcgagtgctt tgaggagaca
    14221 tccatgaatc gcctatgtca ccgattcgcc atcggaacga tgctcacggc cttcgccgcg
    14281 atcgccgtcg cggcgccacc caagaaatcc tacaacctcg aattcctgct gcccgacaac
    14341 gacgagctgc aggcaacgtc gccgacgaac ccggcgtacg actcgacgac ggggcaactg
    14401 attccgccgg tggcggtcac ggtaaccgtc aagaacgaaa gcccgccgtc gaccgccaac
    14461 tcgaacatca gctcgttcca gttcaccgtg tccggcctcg cgctgctcaa cggcagcggc
    14521 gacatcgcat gcccgaatgc acgctgcagc gtcgatccat cgaccggcac cgtttacgtc
    14581 acca
//
LOCUS       DASSVX010000027        13293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_684396, whole genome shotgun sequence.
ACCESSION   DASSVX010000027 DASSVX010000000
VERSION     DASSVX010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 13293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13293
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684396"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFO53_01290"
     CDS             <1..616
                     /locus_tag="VFO53_01290"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HET8874936.1"
                     /translation="RLLDRALAGTIDADEAELFRAQMLTEMARMSVEDGLVMQIHPGS
                     VRNHNPQVFARFGRDKGADIPMRTDYVHALKPLLDSVGNDRALTLILFTLDETAYARE
                     LAPLAGHYPVLKLGPPWWFHDSFQGMLRFLEQAIETAGFANTAGFNDDTRAFASIPAR
                     HDVWRRVVCRFLATAAVEHRLGEDEAMELAPALARGLAKAAYRL"
     gene            691..1029
                     /locus_tag="VFO53_01295"
     CDS             691..1029
                     /locus_tag="VFO53_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874937.1"
                     /translation="MHRAVPAHRIVLTALLSVLLVFLQQESVRHALDHIGAQIERSKH
                     SALERPTGDACLECELLAGGTAGMPVSWVPPLADASAWIEIVAPVARAFVAAPAFYRS
                     RAPPPILRYA"
     gene            1081..2490
                     /locus_tag="VFO53_01300"
     CDS             1081..2490
                     /locus_tag="VFO53_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874938.1"
                     /translation="MFRIPFARAALASALIVAPAAHAASDADLSQLREEIRQLRQNYE
                     ARIEALEARVKEAEAVAKRAAQAQVPAPPAVAALAPPVASTTAAGAPANAANAFNPAI
                     SAVLEGVYSNLSQDPARYGLAGFGLADDVSPGRRGLGLGESEFTLSANVDTRFAGNLT
                     VALTPENTVSVEEAYGLVNGLASGVVPKFGRFFSGIGYLNEQHQHAWDFYDAPLAYQA
                     FLGGQYATDGAQVKWLAPTDQFLEFGAEIGNGDSFPGSARNRNGVGAADVFVHTGGDV
                     GESHSWRAGLSYLDARAGDRDTTQFDAAGNLVQTQFAGKSRLALADFIWKYAPNGNAQ
                     STNFKLQAEYFWRHERGDLTYDTDASLSLTRTAPYASTQSGGYLQGVWQFMPMWRVGL
                     RYDRLNPGDPDYGANDAYLAMPGFHPQRYTFMVDWTPSEFSRVRLQYARNDMRPDVTD
                     DAWFLQYILTLGAHGAHKF"
     gene            2504..3412
                     /locus_tag="VFO53_01305"
     CDS             2504..3412
                     /locus_tag="VFO53_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HET8874939.1"
                     /translation="MNTLLKGLVVLAAFALASPAMATLAVFATVPEWGALAQELGGDK
                     VTVYTATNALQDPHHVEAKPSLIARARKADLVIATGAELEIGWLPLVLQQAGNAKVQP
                     GQPGYFEAASVVTTIERPTRLDRAEGDVHPQGNPHIQTDPRNIARVAAALVQRMADLD
                     PADAALFRGRYASFDARWRASIARWQAEAAPLRGVPVLVQHKAFSYLLDWLGMNETGT
                     LEPKPGVDPTAASLAAVLAEQQRHPATMVLRAAYQSDRASQWIGERAHIAAVALPFTV
                     GGDPAAHDLTSLFDDTIARLLQAARS"
     gene            3409..4182
                     /locus_tag="VFO53_01310"
     CDS             3409..4182
                     /locus_tag="VFO53_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET8874940.1"
                     /translation="MNGVDLSILWPALAAGLLVTATHVPLGMQVLARGIVFIDLAIAQ
                     IAGCGVILADRIGFDAGGASAQVAALIAALAGALLLTWTERRWPDVQEAVIGVVFVMA
                     ATAGVLLLASNVHGSEHLRDLLVGQILWVAPRQLLVTACAYAVVLALWFGARPRLGRT
                     GFYLLFACVVTLSVQLVGLYLVFATLIVPPLATRRVSHGRLARAWAIGIAGYVSGIAA
                     SAAFDIPTGPLIVWTLVAFGVAHFALSSRSRPAGAAVAG"
     gene            complement(4257..4787)
                     /locus_tag="VFO53_01315"
     CDS             complement(4257..4787)
                     /locus_tag="VFO53_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET8874941.1"
                     /translation="MKRRPRAMHLQAALGRAALAIATALLAPGAAAGVYKCALDDGSV
                     TYQENPCPPGKELRDFDRDPAAVSVIPFRIAPGPDTHLVAPRASAAQPASERKSRKSA
                     ERQGNPAERKFLVPGIGEGEVVARIGRPDMSSGAGRKTVRWTYLPAPEDPQTITTLTF
                     ELGRLVEVERKVVRTP"
     gene            4837..6003
                     /locus_tag="VFO53_01320"
     CDS             4837..6003
                     /locus_tag="VFO53_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET8874942.1"
                     /translation="MDASGPLASRVGEIEPFHVMEVQTAARALEAAGRDIVHMEIGEP
                     DFATPQPVLDAARRALAGGHLHYTSALGLPELRGAIARHYAEHYRVDVAPERVIVTAG
                     SSAALLLVMALIVDRDDEILLADPGYPCNRHFVRILEGTPAGIPVSADTRYQLTGELV
                     ERHWRAKTRGVLIASPSNPTGTTVPHDEMRKIAAIVARRGGRLIVDEIYLGLTYGEAP
                     RSVLEFAHDAFIVASFSKYFNMTGWRLGWIVAPERHVRDLEKLAQNLYISPAAPSQRA
                     ALACFEPATAEIVEARRRAFESRRDFLVPALRELGFGVPIMPTGGFFVYADCSRFTDD
                     SERFCREALEGAGVAFTPGIDFGVHRARQHVRMAYTIDERRLEEGVARLRKRLL"
     gene            6013..7206
                     /locus_tag="VFO53_01325"
     CDS             6013..7206
                     /locus_tag="VFO53_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET8874943.1"
                     /translation="MNSEVNPMRRTAPALLRRASSTTDAQRRGRRRRWPIVVAVLAGV
                     VTLAACATFETVDYYWQGAAGQIELLANSQPIPDVIGRSDGALAARLKRIHEIREFAS
                     RELGLPNNGSYTRYTDLGRPYVTWNVFATPELSLKPRQWCFPIAGCVNYRGYFREAQA
                     AGESKRLRAAGDDVYVGGVPAYSTLGWFDDPVLSSFVSWPETEIARLIFHELAHQLIY
                     VKSDSAFNESYATTVEEAGLARWLASRHDSQLDRLAARADHMRGVFRDLVRTTRTKLS
                     EIYASNASDDDKRRAKLEIVAAMKAAYESAKQGDPALSGYDRWFAQQPNNAALAAVGL
                     YTDRVPAFRELLHEANDDLPTFYQRVRELAARPKRQRDAALDALARRAAASAMTAQRS
                     PTHAN"
     gene            7334..7759
                     /gene="rpsF"
                     /locus_tag="VFO53_01330"
     CDS             7334..7759
                     /gene="rpsF"
                     /locus_tag="VFO53_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET8874944.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMIERYRTMVTGPGGRIHRLEDWGR
                     RQLAYPVQKVHKAHYVLMNIECGQETLTELEHAFKFNDAVLRHLVVAMSEAVVTPSPM
                     MKEEKARSLTHGRDAKDAERVPDKPAPESGAPQPAAAQA"
     gene            7775..8092
                     /gene="priB"
                     /locus_tag="VFO53_01335"
     CDS             7775..8092
                     /gene="priB"
                     /locus_tag="VFO53_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HET8874945.1"
                     /translation="MPEINRFTFDAILAACAGVRHTPAGIPAVECTLDHRSQQDEAGS
                     RRKVECEMHAVAFGDVALALGRCAIGSALRCEGFIARRYRTGTSVTLHMTRFGQIDMT
                     KGN"
     gene            8094..8381
                     /gene="rpsR"
                     /locus_tag="VFO53_01340"
     CDS             8094..8381
                     /gene="rpsR"
                     /locus_tag="VFO53_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET8874946.1"
                     /translation="MPRPVGRGKGKFKKDGKRRERGNNLFKRKKFCRFTAENVKQIDY
                     KDIDVLKDFVQENGKIMPARITGTKARYQRQLGTAIKRARFLALLSYTDLH"
     gene            8396..8854
                     /gene="rplI"
                     /locus_tag="VFO53_01345"
     CDS             8396..8854
                     /gene="rplI"
                     /locus_tag="VFO53_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET8874947.1"
                     /translation="MQIILLEKIANVGNLGDVVKVKDGYARNFLIPHGKAKRATPANL
                     EAIEAQRTELERAAGDKLATAQALAEKLEGSTLRIPQKAGVDGRLFGSVTNADIVEAL
                     KAQDVDIEKSMVRMPSGPLKHVGEHPVSVVLHTDVTAHVTVNVVGETIVA"
     gene            8996..12706
                     /gene="dnaB"
                     /locus_tag="VFO53_01350"
     CDS             8996..12706
                     /gene="dnaB"
                     /locus_tag="VFO53_01350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET8874948.1"
                     /translation="MPDDLPLESLKLPPHSVEAEQSVLGGLMLDNEAADKIGDVLGAD
                     DFYSDAHRIIFRHIVQLIGDGKPADVVTVSEALASSQKLDYVGGLAYLGALAQNVPTA
                     ANIRHYANIVRERSILRQLAATATDIAESAFNPLGRSAKTVLDEAEAKVLHIAEQGSR
                     GAQNFQVIGKLLADVVERIETLYNRDDPSDVTGVPTGFADLDRMTSGFQPGDLIVVAG
                     RPSMGKAQPLDARVRAIDGWKRMGDLREGDALASVDGAPSFVSGVFPQGTREVHRVTF
                     SDGRTCECCAEHLWRVHFRGWPAPRVLRTDRVAAMLTRKRYQDRLWIDMPSGDFGHCM
                     DLPLDPWVMGALLGDGNFTGATIRFSTAVEAMRGRMSQRIDEKVGSETTFCSASRQDA
                     TVHQKSSLTPLFYRGHVRGVSGVTQNALTQAVRALGLWGLPSHRKFIPRVFLDASRRD
                     RLDLLRGLLDTDGWVERWGTIRFATASRRLADDMIELVRSLGGWCSVSSKSSRYTYRG
                     ERRDGRESYVCTIHHHDPKSLLLLSDKQARALVAPKRCKRPTFASIVPSRVTQTQCIS
                     VTHPSRLYLTDDYVVTHNTALALNIGENVALNTGMPVAVFSMEMGASQLAMRLIGSVG
                     KLDQHKLRTGRLGPEDWDKLSTALGRLNEAPILIDETPALNAIEVRSRARRLMKQYGK
                     LGLVIVDYLQLMQATTSGENRATEISEISRAMKSLAKELHVPVVALSQLNRSLEQRPN
                     KRPVMSDLRECVTGETLVNLTDGRRVAIRTLVGASPEVVAVGADQRLTAARSDLVWSK
                     GIKPVFKVTLASGRAIRATAGHRLLAHDGWRPVGNLGVGDRVGIARQISEPKAAAEWS
                     DEAVALLGHLVGDGSYLRHQPLRYCTASEENSALVRDAAQALGSTVTRFSGRGAWHQL
                     LIGGNGNRWHPSGVGAWLKSLGIFNQRSHEKHLPEQVFALPNRQLALLAMHLWATDGC
                     IALRRPGTRGAARVYFSTCSDALARDVMALLLRLGIVARLRTTLQEGYRPLHSVDVSG
                     ADGQRRFLDVVWAFGPRLGPATRLRELLRETVANTNADTLPHEVFGFVRQAMGRRGVS
                     QRAMAHARGTSYGGTSHFRFAPSRAHLHSYARLLDDAELARWAHSDLYWDRVVSIDPA
                     GEEEVFDLTVPGPACWLADGIVSHNSGAIEQDADVIVFIYRDEVYNAESQDKGTAEII
                     IGKQRNGPIGTVRLTFLGEYTRFEGFAPPGSY"
     gene            complement(12761..13144)
                     /locus_tag="VFO53_01355"
     CDS             complement(12761..13144)
                     /locus_tag="VFO53_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874949.1"
                     /translation="MLVGVVLAPWCIAHAAPQWSIARPLPKGKQSPLAAAPFQKEAAH
                     DKELAERYPVFFERIEVEGVRDPDKRPAPPRTAEERFADVLNQGNPELVAGKSYDGYY
                     YDGTLFWGSDPLSFAWKNVTHWLGH"
BASE COUNT         2218 a         4460 c         4434 g         2181 t
ORIGIN      
        1 gcggctgctc gaccgcgcac tggcaggcac cattgacgcc gacgaggcgg agttgtttcg
       61 cgcgcagatg ctgaccgaga tggcgcggat gagcgtcgaa gacggcctgg tgatgcagat
      121 tcatccgggc agcgtccgca atcacaatcc gcaggttttc gcgcgcttcg gacgcgacaa
      181 gggtgccgac atcccgatgc gcaccgacta cgtgcatgcg ctgaagccgc ttctggacag
      241 cgtcggcaac gatcgcgcgc tcacgctcat cctgttcacc ctcgatgaaa cggcgtatgc
      301 gcgcgagctg gcaccgctcg ccggtcatta cccggtgctg aagcttggcc cgccgtggtg
      361 gttccacgac agcttccagg ggatgctgcg ctttctcgag caggcgatcg agacggccgg
      421 gttcgcgaac accgcggggt tcaacgacga cacgcgtgcg ttcgcgtcga tccctgcgcg
      481 tcacgacgtg tggcggcgcg tcgtctgccg cttcctcgcc acggcagccg tcgagcaccg
      541 cctcggcgag gacgaggcga tggagctcgc gccggcgctc gcgcgcggcc ttgccaaggc
      601 ggcgtatcgc ctgtaaacag gaactgttgc tccggcaatc ggtcatgcag acgattgcca
      661 cacgcgacgt tcaccgataa tctgcaacac atgcatcgcg ccgttcccgc acatcgcatc
      721 gtcctgacgg cgctgctgtc ggttctgctg gtgttcctgc agcaggaaag cgtgcggcac
      781 gcgctcgatc atatcggtgc gcagatcgag cgctcgaagc actcggcgct cgaacgtccc
      841 accggcgacg cttgtctcga atgcgagctg ctcgccggcg gcaccgccgg gatgcccgtg
      901 tcgtgggtgc cgccgctcgc cgatgcatcg gcgtggatcg aaatcgtcgc gccggtcgca
      961 cgcgcgttcg tcgcggcccc ggcgttttac cgcagtcgcg ctccgcctcc gatcctccgc
     1021 tacgcgtaag ccgtccgccg ccaattgcgg cggtgtcgcg tctttcccgg aggatccatc
     1081 gtgttccgca ttccgttcgc gcgcgccgcg ctggcgtccg cgctgatcgt cgctcctgcc
     1141 gcccacgcgg caagcgacgc cgatttatcg caactgcgtg aggaaatcag gcagcttcgg
     1201 caaaactacg aagcgcgcat cgaagcgctg gaagcacgcg tcaaggaagc cgaggctgtc
     1261 gcgaagcgtg cggcgcaggc gcaggtaccc gcgccaccgg ctgtcgctgc gctggcgcca
     1321 ccggtggcgt cgacaaccgc agccggcgcg ccggcgaacg ccgcgaacgc tttcaatccc
     1381 gcgatctcgg ccgtgctcga aggcgtctac tcgaatctgt cgcaggatcc tgcgcgctat
     1441 ggacttgcgg gattcgggct cgccgacgac gtgtcgcccg gccgccgggg ccttggactc
     1501 ggcgagtcgg aattcacgct gtcggcgaac gtcgacacgc gcttcgccgg caacctcacg
     1561 gttgcgctca cgccggagaa caccgtttcg gtcgaggaag cgtacggtct cgtcaacggg
     1621 ctcgcgagtg gcgtggtacc gaagttcggg cgcttcttct ccggcatcgg ctatctgaac
     1681 gagcagcacc agcacgcgtg ggacttctac gacgcgccgc tcgcctacca ggcgttcctc
     1741 ggcgggcagt acgcaaccga cggcgcacag gtgaagtggc tcgcgccaac cgatcagttc
     1801 ctcgagttcg gcgcggagat cggcaacggc gacagcttcc cgggcagcgc acgcaaccgc
     1861 aacggcgtcg gggccgccga cgtgttcgtg cataccggcg gcgacgtcgg cgaaagccat
     1921 agctggcgcg cgggattgtc gtacctcgat gcgcgcgcag gcgatcgcga cacgacgcaa
     1981 ttcgacgccg cgggcaacct ggtgcagacg caattcgcag gcaagagccg cctggcgctc
     2041 gccgatttca tctggaagta cgcgccgaac ggcaacgcgc agagcacgaa cttcaagctg
     2101 caggccgagt acttctggcg ccacgagcgt ggcgatctca cgtacgacac cgacgcgagc
     2161 ctgagcctca cgcggacggc cccgtacgcg tcgacgcaaa gcggcggcta tctgcagggt
     2221 gtttggcagt tcatgccgat gtggcgcgtg ggcctgcgtt acgaccggct gaaccccggt
     2281 gatcccgact acggcgccaa cgacgcgtat ctcgcgatgc ccggcttcca tccgcagcgc
     2341 tacacgttca tggtcgactg gacgccgtcc gaattcagcc gcgtgcggtt gcaatacgcg
     2401 cgcaacgaca tgcgtcccga cgtcaccgac gacgcgtggt tcctgcagta catcctgacg
     2461 cttggcgcgc acggcgcgca caagttctga acaagaggac gtcatgaaca cattgttgaa
     2521 ggggctcgtg gtgctcgccg cattcgcgct tgcgagcccg gccatggcca cgctcgcggt
     2581 ctttgcgacg gtccccgaat ggggtgcgct tgcgcaggag ctcggcggcg acaaggtgac
     2641 cgtctacacc gccaccaatg cgctgcagga tccgcatcac gtcgaggcga agccaagcct
     2701 gattgcgcgg gcgcggaagg ccgacctcgt cattgcaacc ggtgccgagc tcgagatcgg
     2761 ctggctgccg ctcgtgctgc agcaggcggg caacgcgaag gtgcaacccg ggcagccggg
     2821 ctacttcgag gccgcgtcgg tcgtcacgac gatcgagcgt cccacgcgcc tcgaccgcgc
     2881 cgagggcgat gtgcatccgc agggcaaccc gcatatccag accgatccgc gcaacatcgc
     2941 gcgcgtggcg gccgcgctcg tgcagcggat ggccgacctc gatccggccg atgcggcgct
     3001 gtttcgcggg cgctacgcga gcttcgatgc gcgctggcgt gcgtccattg cgcgctggca
     3061 ggcggaggcg gcgccgctgc gcggcgtgcc ggtgctcgtc cagcacaagg cgttctcgta
     3121 tctgctcgac tggctcggca tgaacgagac cgggacgctc gaaccgaaac ccggcgtcga
     3181 tccgaccgcg gcgtcgctcg ccgcggtgct tgccgagcag cagcggcatc cggcgacgat
     3241 ggtgctccgt gcggcgtacc agagcgaccg cgcatcgcaa tggatcggcg agcgcgcgca
     3301 catcgccgcg gtggcgctgc cgttcaccgt cggcggcgat ccggccgcgc acgacctcac
     3361 gagcctcttc gacgacacga tcgcacgact gctgcaggcg gcccggtcat gaacggcgtc
     3421 gatctgtcga ttctctggcc cgcgctggcc gccgggctgc tggtgacggc cacgcacgtc
     3481 ccgctcggca tgcaggtgct cgcgcgcggc atcgtgttca tcgacctcgc gatcgcgcag
     3541 attgccggtt gcggcgtcat cctcgccgac cgcatcggtt tcgacgcggg cggcgcgtcg
     3601 gcgcaggttg ccgcgctgat cgcggcgctg gccggcgcgc tgctgctcac gtggaccgag
     3661 cgccgttggc ccgacgtgca ggaagcggtc atcggcgtgg tgttcgtgat ggccgccacc
     3721 gcgggcgtgc tgctccttgc aagcaacgtt catggcagcg agcatctgcg cgacctgctg
     3781 gtcggccaga tcctgtgggt ggcaccgcga cagctgctcg ttaccgcgtg tgcctacgcc
     3841 gtggtgctcg cgctgtggtt cggcgcgcgc ccgcggctcg ggcgcacggg tttctatctg
     3901 ttgttcgcgt gcgtcgtgac gctctcggtg caactggtcg ggctgtatct cgtgttcgcg
     3961 acgctgatcg ttccaccgct ggccacgcgc cgcgtgtcgc acggccggct ggcacgcgcg
     4021 tgggcgatcg gcatcgccgg ctatgtcagc ggcattgctg cgtcggcggc gttcgacatt
     4081 cccaccggtc cgctgatcgt gtggacgctg gtggcattcg gcgttgcgca cttcgcgctg
     4141 tcgtcgcgca gcaggccggc cggcgccgcc gtggccggat gacgccgccg cgcaatggga
     4201 agatgacgcg cacccgcgct tcgcgccacg gttgggcaat tgccggcatc cggcgtttac
     4261 ggcgtgcgga cgaccttgcg ctcgacctcg acgagccggc cgagctcgaa cgtcagcgtc
     4321 gtgatggttt gcggatcctc gggtgccgga agatacgtcc agcgcaccgt cttgcggccg
     4381 gcgccggacg acatgtcggg acggccgatg cgtgcgacga cttcgccttc gccgatgccg
     4441 ggaacgagaa acttgcgctc ggcgggattg ccctggcgct cggcgctttt gcgcgacttg
     4501 cgctcgcttg cgggctgcgc cgcggacgcg cgcggggcga cgagatgcgt gtcgggaccg
     4561 ggcgcgatgc ggaacggaat gaccgagacc gcggccggat cgcgatcgaa atcgcgcagc
     4621 tccttcccgg gaggacacgg attttcctgg taggtgaccg atccatcgtc gagcgcacac
     4681 ttgtacacgc ccgcagcagc gccgggcgcg agcagcgcgg ttgcaatcgc gagcgccgca
     4741 cgcccgagtg ccgcttgcag atgcattgcg cggggacgcc gtttcatgac gcgctacgat
     4801 aatcctttcc aatcgtccgc gacactaaga ccttcgatgg atgcttccgg cccgctggcg
     4861 tcccgcgtcg gagaaatcga gccattccac gtgatggagg tgcagaccgc ggcccgtgcg
     4921 ctcgaggcgg ccgggcgcga catcgtgcac atggaaatcg gcgagccgga ttttgcgaca
     4981 ccgcagccgg tgctcgacgc ggcgcggcgc gcgctcgccg gcggccacct gcactacacg
     5041 tcggcgctcg gcctgccgga actgcgcggc gccatagccc ggcactacgc cgagcactac
     5101 cgcgtcgacg tggcgcccga gcgcgtgatc gtcaccgccg gatcgtcggc ggcgttgctg
     5161 ctcgtgatgg cgctgatcgt cgatcgcgac gacgagatcc tgctcgccga tcccggctac
     5221 ccgtgcaacc gccacttcgt gcgcattctc gaaggcacgc cggccggcat tccggtaagc
     5281 gccgacacgc gctaccagct caccggcgaa ctcgtcgagc gtcactggcg cgcgaagacg
     5341 cgcggcgtcc tgatcgcatc gccgtcgaac ccgacgggca ccacggtgcc gcacgacgaa
     5401 atgcggaaga tcgctgccat cgtcgcgcgc cgcggcggcc gcttgatcgt cgacgaaatc
     5461 tacctcgggc tcacgtacgg cgaggcgccg cgcagcgtgc tcgaattcgc gcacgacgca
     5521 ttcatcgtcg cgagcttctc caagtacttc aacatgaccg gatggcggct cggctggatc
     5581 gtggcgcccg agcggcacgt gcgcgatctt gaaaagctcg cgcaaaacct ctacatttcg
     5641 ccggccgcgc cgtcgcagcg cgcggcgctt gcatgcttcg agccggccac cgccgaaatc
     5701 gtcgaagcac ggcgccgcgc cttcgaatcg cggcgcgact tcctcgttcc cgccctccgc
     5761 gaactcggat tcggcgtgcc gatcatgccc accggcggtt ttttcgtcta cgccgattgc
     5821 tcgcgcttca ccgacgacag cgaacgcttc tgccgcgagg cgctcgaagg cgcgggggtt
     5881 gctttcacgc cgggaatcga cttcggcgtg caccgcgccc ggcagcacgt ccgcatggcg
     5941 tacaccattg acgagcgcag gctggaagag ggcgtggccc gcttgcgcaa acgactgctt
     6001 tgaggaatga aagtgaattc cgaagtgaac ccgatgcgcc gcaccgcgcc ggcgctgttg
     6061 cgccgcgcat cgtcgacaac tgacgcgcaa cgtcgcggac gccgccgtcg ctggccgatc
     6121 gtcgtcgccg tgctggcagg ggtcgtcacg ctggcggcgt gtgcgacgtt cgagaccgtc
     6181 gactactatt ggcagggcgc ggcggggcag attgaactgc tcgccaactc gcagccgatt
     6241 cccgacgtca tcggcaggag cgacggggcg ctcgctgcgc gcctgaagcg catccacgag
     6301 attcgcgagt tcgcaagccg cgagctcggg ctgccgaaca acggcagcta tacgcgctat
     6361 accgatctcg gccggccgta tgtgacgtgg aatgtgttcg cgacccccga gctctcgctg
     6421 aagccgcggc agtggtgctt tccgatcgcc ggctgcgtca actaccgcgg ctatttccgc
     6481 gaagcgcagg cggcgggcga atcgaagcgc ctcagggccg ccggcgacga cgtctatgtc
     6541 ggcggcgttc ctgcgtattc gacgctcggc tggttcgacg atccggtgct gtcgtcgttc
     6601 gtgagctggc ccgaaaccga gatcgcgcgc ctcatctttc acgagctcgc ccaccagctc
     6661 atctatgtca agagcgactc cgcgttcaac gagtcgtacg cgacgaccgt cgaggaagcc
     6721 ggactcgcgc gctggctggc ctcgcgccac gattcgcagc tcgacaggct cgccgcgcgc
     6781 gccgatcaca tgcgcggcgt gttccgcgat ctcgtgcgca cgacgcgcac gaagctctcc
     6841 gagatctacg cgagcaatgc gagcgacgac gacaagcggc gcgccaagct cgagatcgtc
     6901 gccgcgatga aggccgcgta cgagtcggcg aagcagggcg atccggcgct gtccggatac
     6961 gaccgctggt tcgcgcagca gccgaacaac gcggcgctcg cggcggtggg cctctatacc
     7021 gatcgcgtgc cggcattccg cgagctgctg cacgaggcga acgacgacct ccccacgttc
     7081 taccagcggg tgcgcgagct cgcggcgcgt cccaagcgcc agcgcgatgc ggcgctcgac
     7141 gcgctggcgc ggcgtgcggc cgcctccgcg atgacggccc agcgctcgcc gacgcacgcg
     7201 aattgatctt ccccgccgct tcctgctaaa ctatcaggct ttccttgcct cacccgggta
     7261 acgttgggga ggccaacagg cgcgacgccc gcaacggcat tcgcgccgaa cagaccgaaa
     7321 ggagatcact ctcatgcgtc actatgagat cgtattcatc gtgcatcccg accagagcga
     7381 gcaggtgccc gcgatgatcg agcgctatcg cacgatggtg accggccccg gcggccggat
     7441 tcaccggctc gaagactggg gacggcgcca gctcgcctat ccggtccaga aagtccacaa
     7501 ggcgcactac gtgctgatga acatcgagtg cgggcaggaa acgctgaccg agctcgagca
     7561 cgcgttcaag ttcaacgatg cggtgctgcg tcacctggtc gttgctatga gcgaagccgt
     7621 cgtcactcct tcgccgatga tgaaggagga aaaggcgcgc tcgctcaccc atggcaggga
     7681 cgcgaaggac gccgagcgcg tgccggacaa gccggcgccc gagagcggcg caccgcagcc
     7741 cgcagcggcg caggcctgat ttcgacgcag ccgcgtgccg gagatcaacc ggttcacgtt
     7801 cgacgcgatc ctcgctgcct gcgccggcgt gcgccacacg cccgccggca ttcccgcggt
     7861 cgaatgcacg ctcgatcacc gctcgcagca ggacgaagcc gggagccggc gcaaggtcga
     7921 atgcgaaatg cacgcggtgg ctttcggcga cgtcgcgctg gcgcttggcc gatgcgcgat
     7981 cggctcggcg ctccgctgtg aaggattcat cgcgcgccgt tatcggaccg gaacgagcgt
     8041 cacgctgcac atgactcgct tcgggcagat cgacatgacg aaaggaaatt gaaatgccac
     8101 gtcccgtggg aagaggcaag ggcaagttca agaaggacgg caagcgccgc gagcgcggca
     8161 acaacctgtt caagcgcaag aagttctgcc ggttcaccgc cgagaacgtc aagcagatcg
     8221 actacaagga catcgacgtc ctgaaggact tcgtgcagga gaacggcaag atcatgcccg
     8281 cacgcatcac gggcacgaag gcgcgctacc agcgccagct cggcacggcg atcaagcgcg
     8341 cgcgtttcct cgcgctgctg tcgtacaccg acctgcacta gcggagcgac ggcacatgca
     8401 aatcattctg ctcgagaaga tcgcgaacgt cggaaacctc ggcgatgtcg tcaaggtgaa
     8461 ggacggttac gcgcgcaatt tcctgattcc gcatggcaag gcgaagcggg cgacgccggc
     8521 caacctggaa gcgatcgagg cgcagcgcac cgagctcgag cgtgccgctg gcgataagct
     8581 cgcgacggcg caggcgcttg ccgaaaagct ggaggggtcg acgctgcgca tcccgcagaa
     8641 ggcgggtgtc gacggccggc tcttcggctc ggtcaccaac gccgacatcg tcgaggcgct
     8701 gaaggcccag gacgtggaca tcgagaagtc gatggtgcgg atgccgtcgg gcccgctgaa
     8761 gcacgtcggc gagcatccgg tgtccgtcgt gctgcacacc gacgtcaccg cgcacgtcac
     8821 cgtcaacgtc gtcggcgaga cgatcgtcgc ctgacgtccg gcggcaccgc gacgcgcacg
     8881 acgaaggggc cggcgggccc cttcgtcgtg tgtgacgacc ggtcgtttgc cgtatgccgg
     8941 attgcgttgc gtcgcagtag aatcgcccgt tccctggcaa gcatcccggc gtaacgtgcc
     9001 cgacgacctt ccgctcgaat cactgaagct gcctccgcat tcggtcgagg cggagcagtc
     9061 ggtgctcggc gggctgatgc tcgacaacga ggccgccgac aagatcggcg acgtgctcgg
     9121 cgccgacgac ttctacagcg acgcgcatcg catcatcttc cggcacatcg tgcaactgat
     9181 cggcgacggc aagccggccg acgtcgtcac ggtgtccgaa gcgctcgcgt cgtcgcagaa
     9241 gctcgactac gtgggcggac tcgcgtatct cggggcgctt gcgcagaacg tgcccacggc
     9301 ggcgaacatc cgccactatg ccaacatcgt ccgcgagcgc tcgatcctgc gccagcttgc
     9361 cgccaccgcg accgacatcg ccgaatcggc gttcaatccg ctcggccgca gcgccaagac
     9421 cgtgctcgac gaggccgagg cgaaggtgct gcacatcgcc gagcagggct cgcgcggcgc
     9481 gcagaacttc caggtcatcg gcaagcttct cgccgacgtc gtcgagcgca tcgagacgtt
     9541 gtacaaccgc gacgatccat cggacgtcac cggcgttccc accgggtttg ccgatctcga
     9601 ccggatgacg tcgggcttcc agccgggcga tctcatcgtc gtggcgggaa gaccgagcat
     9661 ggggaaggcg cagccgctcg atgcacgggt acgtgccatc gatggctgga agcggatggg
     9721 cgatctgcgc gaaggcgacg cgcttgcgtc ggtcgacggc gcaccgtcgt tcgtcagcgg
     9781 ggtttttccg cagggaacgc gcgaagtcca tcgcgtgacg ttctccgacg gtcgcacatg
     9841 cgaatgctgc gccgagcatc tgtggcgcgt tcatttccgc ggctggccgg cgccgcgcgt
     9901 gctgcgcacg gacagggtcg ccgcgatgct gacgcgaaag cgctaccagg atcggctatg
     9961 gatcgacatg ccaagcggcg acttcggcca ctgcatggac ctcccgctcg atccatgggt
    10021 gatgggtgca ctgctcggcg acgggaactt caccggagcg acaatccgct tttccaccgc
    10081 agtcgaggcg atgcgcgggc gcatgagcca gcgcatcgat gaaaaagtgg ggtcagagac
    10141 gactttttgc agcgcttcgc gtcaagacgc aacggttcac caaaagtcgt ctctgacccc
    10201 acttttttat cgcggacacg tgcggggtgt ctcgggcgtt acgcagaacg cgctgacgca
    10261 agcggttcgc gcgctcggcc tgtggggtct gccaagccat cggaagttca tcccgcgcgt
    10321 gttcctcgac gcatcgcgcc gcgaccgcct cgatctgctc cgcgggctcc tcgacaccga
    10381 cggctgggtc gagcgctggg gcaccattcg cttcgcaacc gcaagccgtc ggctcgccga
    10441 cgacatgatc gagctcgtgc gctcgcttgg cgggtggtgc agcgtgtcgt cgaaatcgtc
    10501 acgctacacc tatcgcggtg aacgcaggga cggtcgcgaa agctatgtgt gcacgattca
    10561 tcaccacgat ccgaaatcgc tgctgctgct ctccgacaaa caggcgcgcg cgctcgttgc
    10621 gccgaagcgc tgcaagcgcc cgacatttgc gtcgatcgtt ccgtcgcgcg tgacgcaaac
    10681 gcagtgcatt tcggtcaccc atccatcgcg gctttacctg accgacgact acgtcgtgac
    10741 ccacaacacc gcgctggcgc tcaacatcgg cgagaacgtc gcgctcaata cgggcatgcc
    10801 cgtcgccgtg ttctcgatgg aaatgggcgc ttcgcagctc gcgatgcgat tgatcggatc
    10861 ggtgggcaag ctcgaccagc acaagcttcg caccggccgg ctcggacccg aggactggga
    10921 caagctgtcg acggcgctcg gaaggctgaa tgaagcgccg atcctgatcg acgagacgcc
    10981 ggcgctgaac gccatcgagg tccgctcgcg cgcgcggcgc ctgatgaagc aatacggcaa
    11041 gctcggcctc gtcatcgtcg actacctgca gttgatgcag gcgacgacgt cgggagagaa
    11101 ccgcgccacc gaaatctccg agatctcgcg ggcgatgaaa tcgctcgcga aggagcttca
    11161 cgttccggtc gtcgcgctgt cgcagctcaa ccgctcgctc gagcagcggc cgaacaagcg
    11221 gccggtgatg tcggatctgc gcgaatgcgt gaccggagaa acgctggtca atctgacgga
    11281 cgggcggcgt gtcgccattc gtacgctcgt tggagcgagt ccggaggtcg tggccgtggg
    11341 cgccgatcag cgcctgaccg ctgcgcgctc cgatctcgta tggtcgaagg gcatcaagcc
    11401 cgtgttcaag gtgacgctcg caagtggacg cgcgattcgc gccactgccg ggcaccgact
    11461 gctggcacac gacggttggc gtcccgtcgg aaacctcggg gtcggcgatc gcgtgggtat
    11521 cgcgaggcag atatccgaac ccaaggcagc cgcggagtgg tcggatgaag cggtcgcgct
    11581 gctcggccat ctcgtaggtg atggcagcta tctcaggcat cagccgcttc ggtactgcac
    11641 ggcgtcggag gaaaacagcg cgctggtgcg cgacgccgcg caagcgctgg ggagcacggt
    11701 gacgcgattc agcggaagag gcgcctggca ccaattgctg atcggcggca acggcaatcg
    11761 ctggcatcct tccggcgtcg gtgcatggct gaagtcgctg ggcatcttca accagcgctc
    11821 gcatgaaaag catttgccgg agcaggtctt tgccctgccc aatcggcagc tcgcattgtt
    11881 ggccatgcat ctctgggcca ccgatggatg catcgcgtta cgccggcccg gcacacgcgg
    11941 tgcagcccgg gtttatttca gcacatgcag cgatgcgttg gcccgcgatg tgatggcgtt
    12001 gctcttgcgg ctcggtatcg ttgcccggct gcggaccacg ctccaggaag gctacaggcc
    12061 ccttcatagc gtagatgtga gcggcgcaga cggacagcgg cgatttctgg atgtcgtgtg
    12121 ggcgttcggt cccaggttag ggccggcgac gcgattgcgc gagctgctcc gtgaaacagt
    12181 tgcaaacacc aatgccgaca cgcttccgca cgaagtgttc ggattcgttc gccaggcgat
    12241 gggccggcga ggcgtatcgc agcgtgcgat ggctcacgca cgcggcacgt cgtacggtgg
    12301 aacatcgcat ttccgctttg cgccgagcag ggcgcatttg cattcttacg cgcggttgct
    12361 cgacgacgca gagctggccc gctgggcaca ttccgacctc tactgggacc gcgtcgtgtc
    12421 aatcgatccg gcaggcgaag aagaggtttt cgatctcacc gttcccgggc ccgcgtgctg
    12481 gctcgccgac ggcatcgtga gtcacaactc cggggcgatc gaacaggacg cagacgtcat
    12541 cgtgttcatc taccgcgacg aggtctacaa cgccgaatcg caggacaagg gaacggccga
    12601 gatcatcatc ggcaagcagc gcaacggacc gatcggcacc gtgcgcctca cgtttctcgg
    12661 cgaatacacg cgcttcgaag gcttcgcgcc gcccggcagc tactgacgct gcaccgtccc
    12721 tgccgttcca aagcgacggc gcgcgcaccg tccggcagcg tcagtgccca agccagtgcg
    12781 tgacgttctt ccacgcgaac gacagcggat cggagcccca gaacagcgtg ccgtcgtagt
    12841 agtagccgtc gtagctcttg cctgccacga gctccggatt tccctggttc agcacgtcgg
    12901 cgaagcgctc ctccgccgtt cgcggcggcg cgggccgctt gtcgggatcg cgcacgccct
    12961 cgacctcgat ccgctcgaaa aacaccggat accgctcggc gagctccttg tcgtgcgccg
    13021 cttccttctg aaacggcgcc gcggcgagcg gcgattgctt gcccttcggc agcgggcgcg
    13081 cgatcgacca ttgcggcgcc gcgtgcgcga tgcaccaagg cgccaggaca acgccgacga
    13141 gcatcgcgcg agccgtcaac gaacggtgca cggtggaggg agcgcggaag ggcacgggcg
    13201 gattttacct tcccgggcgg ggaacggcga aacccgccgg atgcgcccgc cgtcggcatt
    13261 gccggcacgc gtcaaaacgc gatctcgtac gca
//
LOCUS       DASSVX010000028        12144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1151025, whole genome shotgun sequence.
ACCESSION   DASSVX010000028 DASSVX010000000
VERSION     DASSVX010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151025"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1602)
                     /gene="rng"
                     /locus_tag="VFO53_01360"
     CDS             complement(145..1602)
                     /gene="rng"
                     /locus_tag="VFO53_01360"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HET8874950.1"
                     /translation="MTHEILINVTPQETRVAMLEQAVVQELHIERASARGLVGNICLG
                     RVARVLPGMQSAFVEIGLERAAFLHIADIWEHRQNGHGEHHPIERILHEGQTLLVQVI
                     KDPIGTKGARLSTQLSLAGRLLVYLPQDSHIGISQRIEGEQERESLRGKLQTLLPEGS
                     AGGFIIRTMAEAASERELSTDVGYLTKLWRDLNERAALVPAPTVLYQDLNLAQRVLRD
                     MSTEETSRVLVDSRETFQRMQAFAREYTPALIDRIEHYAGERPLFDLHGVEDEIEKAL
                     ARRVDLKSGGYLIIDQTEAMTTIDVNTGGFVGGRSFDETIFKTNLEASQVIARQLRMR
                     NLGGIIIIDFIDMENVDHRSAVLAELDKALEKDRTRLTVNGFTQLGLVEMTRKRTRES
                     LAHVLCETCPTCGGRGEFKTAQTICYEILREILREAKQFNAREFRILASQSVIDMFLD
                     EESQSLAQLGDFIGKPISLQVETLYTQEQYDIVLI"
     gene            complement(1643..2257)
                     /locus_tag="VFO53_01365"
     CDS             complement(1643..2257)
                     /locus_tag="VFO53_01365"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET8874951.1"
                     /translation="MSAPVLYLASRSPRRQALLRQLDIEFETLLLREAVGRDRDVVEE
                     TLDAEPAPHYVERMARTKAQVGWQRMHNRKLAERPVLGADTEVVLDGEVFGKPGNADE
                     ATRMIRRLSGRTHQVLTAVALRFRDRTEVEVSISRVTLRRLGAAEINRYVAGGEAFDK
                     AGGYAVQGLAAAFVSRLEGSYSGVVGLPLYETAALLARAGMPVL"
     gene            complement(2275..2742)
                     /gene="rlmH"
                     /locus_tag="VFO53_01370"
     CDS             complement(2275..2742)
                     /gene="rlmH"
                     /locus_tag="VFO53_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET8874952.1"
                     /translation="MKLRVVALGHRMPGWVAAGFDDYARRLPREFALDLVELKPEPRE
                     RGRTVAQILAAEALRIAPACAGLRGVALDEHGEPWSTREFALRLGRWRDAGHDIAFLI
                     GSADGLASEVKSQATAVIALSAMTLPHGLVRVLLAEQIYRAVSVLAGHPYHRG"
     gene            complement(2838..3197)
                     /gene="rsfS"
                     /locus_tag="VFO53_01375"
     CDS             complement(2838..3197)
                     /gene="rsfS"
                     /locus_tag="VFO53_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET8874953.1"
                     /translation="MRLDKLQKVAVAALEDIKARDIQVIDVRPQTSLYDTMVIASGDS
                     NRQVKALAHHVRDKLKEAGASILGIEGEEAGEWVLVDAGDVVVHVMQPAVRAYYNLEE
                     LWAPQARVRRARVASAA"
     gene            complement(3187..3891)
                     /gene="nadD"
                     /locus_tag="VFO53_01380"
     CDS             complement(3187..3891)
                     /gene="nadD"
                     /locus_tag="VFO53_01380"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET8874954.1"
                     /translation="MVNGNDSTPGCIDAPLAMLGGTFDPVHYGHLRLADDVRRALTLA
                     EVRLVPAADPPHRPPPKAGARDRVAMLELGVREFPGLVVDTREIARGGKSYTVDTLAA
                     LRMDLPRTPLLLLLGADQFRSLPSWHRWQALFDLAHLVVVPRPGVAIGEGLPAAVARE
                     WRARHLDDRQGLRSRIAGSIYVQPVTPQPISSTAIRAAFARSGGHPVEIAGLLPPAVL
                     AYIESNGLYTHPTHAS"
     gene            complement(3891..4502)
                     /locus_tag="VFO53_01385"
     CDS             complement(3891..4502)
                     /locus_tag="VFO53_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HET8874955.1"
                     /translation="MLDPLANGILDFWFVPRPGERNAAEASPAPAGLVTREVWFRKDD
                     AFDDEIRERFGVVLAAGIAGAFGDWCNEPRGCLARVVLLDQFTRNAFRGTPDAFAGDP
                     GALATAEDAIERRFDRVLAPHERWFLYMPFEHSEALAQQDRAVALFEALAAQTGLDEP
                     LPWARRHRDVIRRFGRFPHRNDILGRASTPEEVAFLKEPGSRF"
     gene            4563..5744
                     /locus_tag="VFO53_01390"
     CDS             4563..5744
                     /locus_tag="VFO53_01390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8874956.1"
                     /translation="MQTSRRDIFLLACCQALLLINSSGLIAMNGLIGYSLAQTKTLAT
                     FGVTTYVLGSALATMPMSLWMGRAGRRAGFMAGALINVGGCIIGAWALAIHSFALFCA
                     ATGIIGVYNAVGLQYRFAAAEVATAGDRAKAISLVLAGGVAGGLIGPQSVRVARDLFA
                     TPFLGSFLVLALFALVALAVQSRVHVPRPSHDERTGRARPLGVIVRQPAFVVAVLAGA
                     LGYGLMNLLMTATPIAMNFCGHPFGAMALVIEWHVVAMFAPGFVTGSLIRRFGTLTII
                     LAGVALTAIATLIALDGNSVAHFVAALVAVGVGWNFMYTGGTALLTETYAPSEKARVQ
                     GLNDFIVFATMGVSSLASGALVTTSGWETMNRATLPVLGVIAAGVLWLMWLRRQAPAA
                     A"
     gene            complement(5800..6753)
                     /locus_tag="VFO53_01395"
     CDS             complement(5800..6753)
                     /locus_tag="VFO53_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HET8874957.1"
                     /translation="MAAASLLALIDDIASVLDDVALLTKLAAKKTAGVLGDDLALNAQ
                     QVAGVRSDRELPIVWAVAKGSLINKAILVPSALAISAFFPWAVTPLLMLGGLFLCYEG
                     FEKIAHRLAGTHRAEVAAEHRRLAGALADPRVNIVDFEKDKIRGAVRTDFILSAEIIT
                     ITLGTVATAGFATRVAVLIGIAVLMTVGVYGLVAGIVKLDDAGLLLSERAGDRAIARV
                     QRAVGAGIVRSAPYLMKGLSVAGTAAMFLVGGGILTHGVPVVHHAIENWGESAGDIPA
                     IGAVFGALAPMLLNAAAGIAAGAIVYAVLAVNKWGQRRISL"
     gene            complement(6866..8158)
                     /locus_tag="VFO53_01400"
     CDS             complement(6866..8158)
                     /locus_tag="VFO53_01400"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET8874958.1"
                     /translation="MNDLADTQIAQIADVAGYMHRVGEAARAAARHLARADTNAKNVA
                     LARAASALRRDASRLMQANARDVEAACAAGHDAAFVDRLTLTSPRIEAMARGLDEIAA
                     LADPVGEISDLRYRPSGIQVGTMRVPLGVIGIVYESRPNVTADAAGLCLKSGNATILR
                     GGSEAIHSNRAIARCVHEGLRDAGLPEHAVQLVDTTDRAAVGHLIADDVHVDVIVPRG
                     GKSLIERVTRDARVPVLKHLDGVCHVYVDAAANVEQAIRIADNAKTQRYSPCNTMETL
                     LVHAEIADRVLPPLAAIYAQKGVEMRGDERARALVPAMKVATEDDWYAEYLAPIIAIR
                     VVDSLDAAIDHIARYGSQHTDAIVTDDYVSAMRFLREVDSSSVMVNASTRFADGYEYG
                     LGAEIGISTNKLHARGPVGLEGLTSRKWIVLGSGQIRS"
     gene            complement(8194..8859)
                     /gene="rpiA"
                     /locus_tag="VFO53_01405"
     CDS             complement(8194..8859)
                     /gene="rpiA"
                     /locus_tag="VFO53_01405"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET8874959.1"
                     /translation="MTQDEQKQLVAREALRYVVEDVWLGVGSGSTANMFIDALAASRL
                     RIKGAVASSVKTAERLKAHRIDVEELNNVSDIPVYIDGADEVTAGGAMIKGGGGALTR
                     EKIVAAVARKFVCIADQSKKVDVLGSFPLPVEVIPMARSYVARELVRLGGYPEWRMGF
                     TTDNGNVILDVSDLAITDPIALEDRINAIAGVVTVGLFARRGADVILLGAPEGVDTIT
                     PNR"
     gene            9053..9384
                     /locus_tag="VFO53_01410"
                     /pseudo
     CDS             9053..9384
                     /locus_tag="VFO53_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248451.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            9946..10101
                     /locus_tag="VFO53_01415"
     CDS             9946..10101
                     /locus_tag="VFO53_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874960.1"
                     /translation="MRNDKALARVMIAVLKDDTELFRQFMDNESFRRWLTDTVFGLTY
                     DAAAAGA"
     gene            complement(10359..>12144)
                     /gene="aceF"
                     /locus_tag="VFO53_01420"
     CDS             complement(10359..>12144)
                     /gene="aceF"
                     /locus_tag="VFO53_01420"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HET8874961.1"
                     /translation="GDFTDVPVIEVFVKPGDQVGVDDSLVTLESDKAAMDVPSPAAGK
                     VARIAVKVGDKVSKGSLVLTLQGDGQAAAQAPAANRKASAADASEGVSHASPRAGTTS
                     AAPAPELVTPAPRGEKGRADMPGEGSNSTPPAGATIEVRVPDIGDFNDVPVIEVFVKP
                     GDIVQAEDPLVTLESDKATMEVPAPIGGVVEGVRVGVGDRVSEGTPLLTLKTSQTGAE
                     PASRSTPPSYPDNADRAIPPVEASPSGAPAGVEARANESAATSREGVSRPVQRPDVQQ
                     QAGAAATAQRPAGAAHASPSVRKFARELGVDIARVSGTGPKGRIVHEDVQQFVKAALA
                     AAPGAPGTGAAGAGGLSVLPWPRIDFTKFGSVETKPLSRIGKISGANLARNWVMIPHV
                     TQFDDADITELEALRVALNKENEKAGVKITMLAFLIKAAVASLKHFPDFNASLDAGGE
                     NLVRKHYFNIGFAADTPNGLVVPVVKGADGKGVLAIAKEMSELSAKARDGKLGPADMQ
                     GGCFSISSLGGIGGTAFTPIINAPEVAILGVSRSAMKPVWDGKAFAPRLMLPLSLSYD
                     HRVIDGAQAARFITYYASLLADFRRALL"
BASE COUNT         1909 a         4135 c         4048 g         2052 t
ORIGIN      
        1 ggatgcgcag ccacagctgc ttcatgcggt catcggcgtg ccaccgccgt taccgcgcga
       61 tccgcgcagg gccgccccaa gccgattgcg gaatctttcc ggacgaaagt atcgtgcgag
      121 tgcaacgagc gaaactttcg ctcgctaaat tagtacgatg tcgtactgct cctgtgtgta
      181 gagcgtctcg acctgcagcg agatcggctt gccgatgaag tcgcccagct gcgcgaggct
      241 ttgcgactcc tcgtcgagga acatgtcgat caccgactgt gacgcgagga tgcggaactc
      301 gcgcgcgttg aactgcttcg cctcgcgcag gatctcgcgc aggatctcgt agcagatcgt
      361 ctgcgccgtc ttgaactcgc cgcgcccgcc gcacgtcggg caggtctcgc acagcacgtg
      421 cgcgagcgat tcgcgcgtgc gcttgcgcgt catctcgacc aggccgagct gcgtgaagcc
      481 gttgacggtg aggcgcgtgc ggtccttctc gagcgccttg tcgagctcgg ccagcacggc
      541 gctgcgatgg tcgacgttct ccatgtcgat gaagtcgatg atgatgatgc cgccgaggtt
      601 gcgcatccgg agctggcgcg cgatcacctg cgacgcttcg aggttcgtct tgaagatcgt
      661 ctcgtcgaag ctgcgcccgc cgacgaagcc gccggtgttg acgtcgatcg tggtcatcgc
      721 ctccgtctgg tcgatgatca ggtagccgcc cgacttgaga tcgacccggc gtgcgagcgc
      781 cttctcgatc tcgtcctcga cgccgtgcag gtcgaacagc ggccgctcgc ccgcgtaatg
      841 ctcgatcctg tcgatcagcg ccggcgtgta ctcgcgcgcg aacgcctgca tcctctggaa
      901 cgtctcgcgc gaatcgacga ggacacgcga ggtttcctcg gtcgacatgt cgcgcagcac
      961 ccgctgcgcg agattgagat cctggtagag caccgtcggc gcgggaacga gcgccgcgcg
     1021 ctcgttcagg tcgcgccaaa gtttcgtcag gtagccgacg tcggtcgaaa gctcgcgctc
     1081 gctcgccgcc tcggccatcg tgcggatgat gaagccgccg gcggagccct cgggcagcag
     1141 cgtctgcagc ttgccgcgca ggctttcgcg ctcctgctcg ccctcgatcc gctgcgaaat
     1201 gccgatatgc gaatcctgcg gcaggtagac gagaagccgc ccggcgagcg aaagctgcgt
     1261 ggacagccgc gcgcctttgg tgccgatcgg atccttgatc acctgcacga gcagcgtctg
     1321 gccctcgtgc aggatgcgct cgatcgggtg atgctcgccg tggccgttct ggcgatgctc
     1381 ccagatgtcg gcgatgtgca ggaacgctgc gcgctcgagg ccgatctcga cgaacgcgct
     1441 ttgcatgcca ggcagcacgc gtgcgacgcg accgaggcag atgttgccga cgaggccgcg
     1501 cgcgctcgcg cgctcgatgt gcagctcctg cacgaccgcc tgctcgagca tcgcaacgcg
     1561 cgtctcctgc ggcgtcacgt tgatgagaat ctcgtgggtc attggacccc cacgcttgcg
     1621 cctcggggcg gccctttggc gctcataaca ccggcattcc ggcacgagcg agcagcgccg
     1681 ccgtctcgta gagcggcagg ccgacgacgc cgctgtagct gccttcgaga cgcgagacga
     1741 acgccgccgc aagcccctgc acggcgtatc cgccggcctt gtcgaaagcc tcgccgcccg
     1801 ccacgtagcg attgatctcg gcggcgccga ggcggcgcag cgtcacgcgc gagatcgaca
     1861 cctccacttc ggtcctgtcg cgaaagcgaa gcgcgaccgc ggtgagcacc tggtgcgtgc
     1921 gtcccgacaa gcggcggatc atgcgcgttg cttcgtcggc gttgcccggc ttgccgaaca
     1981 cctcgccgtc gagcacgacc tcggtgtcgg cgccgagcac cggccgctcc gccagcttgc
     2041 gattgtgcat tcgctgccag ccgacctgcg ccttggtgcg cgccatgcgt tccacgtaat
     2101 gtggcgcagg ctctgcgtcg agcgtttcct cgacgacgtc gcggtcgcga cccaccgcct
     2161 cgcgcagcag cagcgtctcg aactcgatgt cgagctggcg cagcagcgcc tgccgccgcg
     2221 ggctcctgga cgcgagatac agcaccggtg cgctcatctt cgctctcccc tgggtcagcc
     2281 gcgatgataa gggtgtccgg cgagaacgct cacggcgcga tagatctgct cggcgaggag
     2341 cacccgcacg agcccgtgcg gcagcgtcat ggccgacagc gcgatcaccg ccgtcgcctg
     2401 cgatttcact tcgctcgcga ggccatcggc gctgccgatg aggaaggcga tgtcgtggcc
     2461 cgcatcgcgc cagcggccaa gccgcagcgc gaattcgcgc gtcgaccacg gctcgccgtg
     2521 ctcgtcgagg gcgacgcctc gaaggcccgc gcaggcgggc gcaatgcgca atgcttcggc
     2581 ggcgaggatc tgcgcgaccg tgcgtccgcg ctcgcgcggc tcgggcttga gctcgacgag
     2641 atcgagcgcg aattcgcgcg ggagccggcg tgcgtagtcg tcgaagccgg cggcgaccca
     2701 gccgggcatc cgatgtccga gcgcaacgac gcgcagcttc atcaggacct aagcagggtc
     2761 agagacgact ttcgcgagcc ggcaggcgat gcgacacggc tgcgccgcga aagtcgtctc
     2821 tgaccctgct caggtgctta cgccgccgat gcgacgcgag cgcgccggac gcgcgcctgc
     2881 ggtgcccaga gctcctcgag gttgtagtag gcgcgcacgg ccggctgcat gacgtggacg
     2941 acgacgtcgc ccgcgtcgac gagcacccat tcgccggcct cctcgccctc gatgccgagg
     3001 atcgaagccc cggcttcctt gagcttgtcg cgcacgtgat gggcgagcgc cttcacctgg
     3061 cgattcgagt cgcccgaggc gatcaccatc gtgtcgtaca gggaggtttg cggacggaca
     3121 tcgattacct ggatgtcgcg ggccttgatg tcctcgagtg ccgcgacggc gaccttttgc
     3181 agcttgtcaa gacgcatgcg ttgggtgtgt gtagagtccg ttcgattcaa tataggctaa
     3241 aaccgctggc gggagcaaac cggcaatttc aaccggatga ccaccgctgc gggcaaatgc
     3301 cgcgcgtatc gcggtcgacg aaatcggctg cggcgtcacg ggctggacgt agatggagcc
     3361 ggcgatccgt gagcgcaacc cctgccgatc gtcgaggtgc cgcgcccgcc attcccgcgc
     3421 aaccgcggcg ggaagccctt cgccgatcgc gacccccgga cgcgggacga cgacgagatg
     3481 cgcgaggtcg aacagcgcct gccagcgatg ccacgacggc aggctgcgaa actgatccgc
     3541 gccgagcagc agcagcagcg gtgtgcgggg caggtccatg cgcagtgcgg cgagcgtgtc
     3601 gaccgtgtag cttttcccgc cgcgcgcgat ttcgcgcgta tcgacgacga gccccggaaa
     3661 ttcacgcacg ccgagctcga gcatcgcgac ccggtcgcgc gcgccggcct tcggtggcgg
     3721 ccggtgcggc ggatcggccg cgggcacgag acgcacctcg gcgagcgtga gcgcgcgtcg
     3781 cacgtcgtcg gcgaggcgca agtgcccgta gtgcacagga tcgaacgtgc cgccgagcat
     3841 ggccagcggc gcatcgatgc atccgggcgt cgagtcgttg ccgttcacca tcaaaagcgc
     3901 gagccgggct ccttcaggaa cgcgacttcc tcgggcgtcg aggcgcggcc gagaatgtcg
     3961 ttgcgatgcg ggaagcggcc gaagcggcgg atcacgtcgc gatgacgccg cgcccacggc
     4021 agcggctcgt cgagccccgt ctgcgccgcg agcgcctcga acagcgcaac cgcgcgatcc
     4081 tgttgcgcga gcgcttcgct gtgctcgaac ggcatgtaca ggaaccagcg ctcgtgcggc
     4141 gcaagcacgc ggtcgaagcg gcgctcgatc gcgtcctcgg cggtggcaag cgcacccggg
     4201 tctcccgcga acgcatcggg cgtgccgcga aatgcgttgc gcgtgaactg atcgagaagc
     4261 acgacgcgcg caaggcatcc gcgcggctcg ttgcaccagt cgccgaatgc gcccgcaatg
     4321 ccggccgcga gtaccacgcc gaagcgctcg cggatctcgt cgtcgaatgc atcgtccttg
     4381 cggaaccaga cctcgcgcgt gacgagcccg gcaggcgccg gcgacgcttc ggctgcattc
     4441 cgctcgccag ggcggggaac gaaccagaaa tcgaggatgc cgttcgcgag cgggtcgagc
     4501 atggtcctgc gcttatcata cggccttttg cgcgcgcggc cgcgaagggc gcgtgcctct
     4561 tcgtgcagac ctcgcgccgc gacattttcc tcctcgcctg ttgccaggcg ttgctgctca
     4621 tcaattcgtc ggggctgatc gcgatgaacg gcctcatcgg ctattcgctc gcgcaaacca
     4681 agacgctcgc cacgttcggc gtgacgacgt acgtgctggg ctcggcgctc gcgacgatgc
     4741 cgatgtcgct gtggatgggg cgtgcgggcc gccgcgccgg gttcatggcc ggcgcgctga
     4801 tcaacgtggg cggctgcatt atcggcgcgt gggcgctcgc gattcacagc ttcgcgcttt
     4861 tctgcgcggc caccggcatc atcggtgtct acaacgccgt cggcctgcaa taccggttcg
     4921 ccgcggccga agtggcgaca gccggcgacc gcgcaaaagc gatctcgctc gtgcttgccg
     4981 gcggcgtcgc cggtggtctc atcgggccgc aatcggtgcg cgtggcgcgc gatctgttcg
     5041 caacgccgtt cctgggctcg ttcctggtgc tcgcgttgtt cgcgctcgtc gcactcgccg
     5101 tgcagtcgcg cgtgcacgtg ccgcggccgt cgcacgacga gcgcaccggc agggcgcggc
     5161 cgcttggcgt gatcgtgcgc cagcccgcgt tcgtcgtggc cgtgctggcg ggtgcgctgg
     5221 gatacgggct catgaacctg ctgatgaccg cgacgccgat cgcgatgaat ttctgcggcc
     5281 atccgttcgg cgcgatggcg ctcgtcatcg aatggcacgt cgtcgcgatg ttcgcgccgg
     5341 gcttcgtcac cggctcgctg atccgccgct tcggcacgct cacgatcatc ctcgccggcg
     5401 tcgcgctgac cgcaatcgcg acgctgatcg cgctcgacgg caattcggtt gcgcatttcg
     5461 tcgccgcact cgtcgccgtc ggcgtgggct ggaacttcat gtacaccggc ggtaccgcgc
     5521 tactgaccga aacgtacgca ccaagcgaga aggcgcgggt gcaggggctc aacgatttca
     5581 tcgtcttcgc gacgatgggc gtgtcgtcgc tcgcgtccgg cgccctcgtc acgacgagcg
     5641 gatgggagac gatgaaccgc gcgacgctgc cggtgctcgg cgtcatcgcg gccggcgtgc
     5701 tgtggctgat gtggttgcgc cgccaggcgc ccgcggcggc ctagaataaa tggggtcaga
     5761 gacgaatatc gcgatacgac ggcatggcat gaaacgactt cacagcgaaa ttcgtctctg
     5821 accccattta ttcacggcga gcaccgcgta gacgatcgcg ccggccgcga tgcccgccgc
     5881 ggcattgaga agcatcggtg cgagcgcgcc gaacacggcg ccgatcgccg gaatatcgcc
     5941 cgcgctctcg ccccagttct cgatcgcgtg atgcacgacg ggtacgccgt gcgtcaggat
     6001 gccgccgccg accaggaaca tcgccgcggt gccagcaacg gacaggccct tcatgaggta
     6061 gggcgccgag cggacgatgc cggcgccgac cgcgcgctgc acgcgagcga ttgcccggtc
     6121 gccggcgcgt tcggagagca gcaggccggc atcgtcgagc ttgacgatgc cggcaacgag
     6181 cccgtagacc cccaccgtca tgaggaccgc gatgccgatg agcaccgcga cccgggtcgc
     6241 gaatccggcc gtcgcgacgg tgccgagcgt gatcgtgatg atctcggccg acagaatgaa
     6301 gtcggtgcgc accgcgccgc ggatcttgtc cttctcgaag tcgacgatgt tcacgcgcgg
     6361 gtcggccagc gcgccggcga gccgccggtg ctcggccgcg acttccgcgc ggtgcgtgcc
     6421 cgcaagccgg tgtgcgatct tctcgaagcc ttcgtagcac aggaacaggc ccccgagcat
     6481 caaaagcggc gtcaccgccc acggaaagaa cgcgctgatc gcgagtgcgg acggaaccag
     6541 gatcgccttg ttgatgagcg accctttcgc caccgcccac acgatcggca actcgcgatc
     6601 ggacctgacg cccgcgacct gctgtgcgtt cagcgcgagg tcgtcaccga ggacgcccgc
     6661 ggtcttcttc gcggcgagct tggtgagcag cgccacgtcg tcgagcacgc tggcgatgtc
     6721 gtcgatcagc gcaaggaggc ttgcggcagc caaggtatcg cgctatccgg gtttgggagg
     6781 gctgcctggc tgccggaggc gccgctcgaa cgacacgatc gcgcggcagc ccggcgtccg
     6841 cgcaatggcg cctgcgacgc cgaattcaac tgcggatctg tccgctgccg agcacgatcc
     6901 acttgcggct cgtcaggccc tcgagaccga ccggtccccg cgcgtgcagc ttgttcgtcg
     6961 agatgccgat ttccgcgccc agcccgtact cgtagccgtc ggcgaagcgg gtcgacgcat
     7021 tcaccatcac cgagctcgaa tcgacctcgc ggagaaagcg cattgcgctc acgtagtcgt
     7081 cggtgacgat ggcgtcggtg tgctgcgagc cgtagcgcgc gatatggtcg atcgccgcgt
     7141 cgagcgaatc gacgacgcgg atcgcaatga tcggcgcgag gtattcggcg taccagtcgt
     7201 cctcggtcgc gaccttcatc gccgggacga gcgcgcgcgc acgttcgtcg ccgcgcatct
     7261 cgacaccctt ctgcgcatag atcgcggcga gcggcggcag cacgcggtcg gcgatctcgg
     7321 catggacgag cagcgtctcc atcgtgttgc acggcgaata gcgctgcgtc ttcgcgttgt
     7381 cggcgatgcg gatcgcctgc tcgacgttgg cggcggcatc gacgtagacg tggcacacgc
     7441 cgtcgagatg cttcagcacg gggacgcgcg cgtcgcgggt gacgcgctcg atcagcgact
     7501 tcccgccgcg cggaacgatg acgtcgacgt gcacgtcgtc ggcgatcagg tggccgacgg
     7561 cggcacggtc ggtcgtgtcg acgagttgca ccgcatgttc cggcaagccc gcgtcgcgca
     7621 gcccctcgtg cacgcatcga gcgatcgcgc ggttgctgtg gatcgcctcg gaaccgccgc
     7681 gcaggatcgt cgcgttgccc gacttgaggc acaggcccgc ggcgtcggcg gtgacgttcg
     7741 gccgcgattc gtacacgatg ccgatcacgc cgagcggcac gcgcatcgtg ccgacctgga
     7801 tgcccgacgg gcgatagcgc agatcggaaa tctcgccgac cggatcggcc agcgccgcaa
     7861 tctcgtccag gccgcgcgcc atcgcttcga tgcgcggcga cgtgagcgtc aaccggtcga
     7921 cgaacgcggc gtcgtgcccg gctgcgcagg cggcttcgac gtcgcgcgca ttcgcctgca
     7981 tcagccggct cgcatcgcgg cgcagtgcgc tcgccgcacg cgcgagtgcc acgttcttcg
     8041 cgttcgtgtc cgcgcgtgcg agatggcgcg ccgcggcgcg cgccgcttcg ccgacgcgat
     8101 gcatgtagcc ggcgacgtcg gcgatctgcg cgatttgcgt gtcggcgaga tcgttcatcg
     8161 gaccattcta gcgcgcggat gtccggcgtc ggcctatcgg ttcggcgtga tcgtatccac
     8221 gccttccggc gccccgagca gaatgacgtc cgcgccacgc cgtgcgaaga gtccgacggt
     8281 cacgacgccg gcgatcgcgt tgatgcggtc ttcaagcgcg atcgggtcgg tgatcgcaag
     8341 atccgatacg tcgaggatca cgttaccgtt gtccgtcgtg aagcccatgc gccattcggg
     8401 gtagccgccg agccgcacga gctcgcgcgc gacgtagctt cgcgccatcg gaatgacctc
     8461 gaccggcaat ggaaagctgc cgagcacatc gaccttcttc gactggtcgg cgatgcagac
     8521 gaacttccgc gcgacggcgg cgacgatctt ctcgcgcgtc agcgcgccgc cgccgccctt
     8581 gatcatcgcg ccgcccgcgg taacctcgtc ggcgccgtcg atgtagaccg ggatgtcgct
     8641 gacgttgttc agttcctcga cgtcgatgcg gtgtgccttc agccgctcgg ccgtctttac
     8701 cgagctcgcg acagcgccct tgattcgaag gcggcttgcg gcgagcgcgt cgatgaacat
     8761 gttcgcggtc gagcccgacc cgacgcccag ccacacgtcc tcgacgacgt agcgcagcgc
     8821 ctcccgcgcg acgagctgct tttgttcgtc ctgggtcatg atctgcgtcg ccattttcgc
     8881 acggcccgtt ggttgctgac cgcaggtgcg tggtttgaac gttgcttcgc cggtgctagc
     8941 atccgagccc ggaagagagg gaagaatcat cgttcgcgcg ctggcactcg acgaggacgg
     9001 tccgacgcgg cgcaagttcc tcgcgcgcct gcagggtgaa atcagcaagc gcgagcacga
     9061 gtacggaggc gtcaaggtta ccgaggcgtc gctgaaccgc ttcccgtcga gccagattgc
     9121 ggacaggatt caggaagacc gtaccgtttc ctgatctgcg ccgacaagtt ccagacgggc
     9181 tacgacgagc ctgctgcaca cgatgtacgt ggggacaagc cgctgtccgg aatcagggcg
     9241 gtgcagaccc tctcgcggct gaatcgcgcc tacccgaaga aacacggcgt gttcgtgctc
     9301 gacttcatga acgacgccga cacgatccgg aaggcgttcg ccgactacta ccgcacgacg
     9361 atcctcgcca aggagaccga cccgctcggc cggtgcttga gtgctcaagg acttggatgg
     9421 ccactcgacc atatgccgtg cgcaaaaaag gcccgccgac acgacgtcgt cctgcccgcg
     9481 gcgtgcttta cgacgagcga ttgttcagct cgagggaaac cacgggcgcg cgacgtcggc
     9541 atcggcatcg gtaggaaggg ccgacgtagc ccggatgatc atcgtctcgt tgaccacgcg
     9601 ttggacgccc ggcacgcgac gcaccgcatc ggcagcaccc cacgactcgg cttccgagcg
     9661 aacattgccg gtcaggacgg ctacgccatc ttgaactcga atttcgattc tttccgcatc
     9721 gacaccagac tcccgcacaa gctcctgtcg agcgtcatgc tgcaattcgc tatccgacct
     9781 cattggcggc tccttcgaga aatgagccat cgattctcgg cacctcgggc tccaggcttc
     9841 gacactacgc gcacgtgcag gatttgcgaa taggacaatc tccaccgcgg gtgtcgggca
     9901 ttctcgaatc cggccggctc gaaccccctg ccaaacgaac tgttcatgag gaacgacaag
     9961 gcgctcgccc gcgtcatgat cgcggtgctc aaggacgaca ccgagctgtt caggcagttc
    10021 atggacaacg agtcgttcag gcgctggctg accgacacgg tattcgggtt gacgtacgac
    10081 gcggccgcgg ccggtgcttg aaccgccaac cgcgcgcggc cacgccgatc gaatgtcgca
    10141 aaaaaacgcg ccggcccgtt caagatcaat gcctgattgg agcgatcgac cataagtctg
    10201 atcgctacaa ctcattgacg atcaatgaat tccgtcgcgt gattgtgtga tcaccggctg
    10261 attcgcggca ctgatcatgc atgggcggca gcaaaatttt tcgaggtgcc gccacaggtt
    10321 ttcccggtgc tccgacgagg ttcgcgggct tcgcgcgctc acagcagcgc gcggcggaaa
    10381 tccgccagca ggctcgcgta atacgtgatg aaccgcgccg cctgcgcgcc gtcgatcacg
    10441 cggtggtcgt acgacagcga cagcggcagc atcagccgcg gcgcgaacgc cttgccgtcc
    10501 cacaccggct tcatcgcgct gcgcgacacg ccgaggatcg ccacttccgg agcgttgatg
    10561 atcggcgtga atgcggtgcc gccgatgccg ccgagcgacg aaatcgagaa gcagccgccc
    10621 tgcatgtccg caggcccgag cttgccgtcg cgcgccttcg ccgacagctc ggacatttcc
    10681 ttcgcgatcg cgagcacgcc cttgccgtcg gcgcctttca cgacgggaac gacgagcccg
    10741 ttcggtgtgt cggcggcgaa gccgatgttg aagtagtgct tgcggaccag attctcgccg
    10801 cccgcgtcga gcgacgcgtt gaaatcggga aaatgcttca acgacgcgac ggcggccttg
    10861 atcaggaacg cgagcatcgt gatcttgacg ccggccttct cattttcctt gttgagcgcg
    10921 acgcgcaatg cctcgagctc ggtgatgtcg gcgtcgtcga actgcgtgac gtgcggaatc
    10981 atcacccagt tgcgtgcgag attggcgccg gaaatcttcc cgatgcgcga cagcggcttc
    11041 gtctccaccg aaccgaactt cgtgaagtcg atgcgcggcc aggggaggac cgacagtccg
    11101 ccggcaccgg ccgcgcccgt gcccggtgcg cccggcgccg ccgcgagcgc ggctttcacg
    11161 aactgctgca cgtcctcgtg gacgatccgt cccttcggcc ccgttccgct cacccgcgcg
    11221 atgtcgacgc cgagctcgcg tgcgaacttg cgcaccgacg gcgatgcgtg ggcggcaccg
    11281 gccgggcgct gcgccgtggc ggccgcaccg gcctgctgct ggacgtcggg ccgctgcacc
    11341 ggccgtgaca cgccttcgcg cgaagtggcc gcgctttcgt tcgctcgcgc ttccaccccc
    11401 gcgggtgcgc ccgaaggcga cgcttccacc ggcgggatcg cgcgatccgc gttgtcggga
    11461 tagctcggcg gcgtgctgcg gctcgccggc tcggcgccgg tttgcgacgt cttcagcgtc
    11521 aaaagcggcg tgccttcgct gacgcggtcg ccgacgccga cgcgcacacc ctcgacgacg
    11581 ccgccgatcg gcgccgggac ctccatcgtc gccttgtccg attcgagcgt caccagcgga
    11641 tcctcggcct gcacgatatc gccgggcttg acgaacacct cgatcaccgg aacgtcgttg
    11701 aagtcgccga tgtcgggcac acgcacttcg atcgtcgctc ccgcgggcgg cgtcgagttc
    11761 gacccttcgc ccggcatgtc ggcgcggccc ttttctcccc gcggcgcggg cgtgacgagc
    11821 tcgggcgcgg gcgccgcgga ggttgttccg gcgcgcggcg atgcgtgcga cacgccttcg
    11881 cttgcatcgg cggcgctcgc tttgcggttt gccgcaggcg cttgcgccgc cgcctggccg
    11941 tcgccttgca gcgtgagcac cagcgatccc ttgcttacct tgtcgccgac tttcaccgcg
    12001 atcctcgcaa ccttgccggc ggcgggcgac ggaacatcca tcgccgcctt gtccgattcg
    12061 agcgtgacga gcgaatcgtc gacgccgacc tggtcgccgg gcttcacgaa cacctcgatg
    12121 accggcacat cggtgaagtc gccg
//
LOCUS       DASSVX010000029         5722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1334339, whole genome shotgun sequence.
ACCESSION   DASSVX010000029 DASSVX010000000
VERSION     DASSVX010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334339"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VFO53_01425"
     CDS             complement(<1..526)
                     /locus_tag="VFO53_01425"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HET8874962.1"
                     /translation="MLDAYLQPLPLVAVLRGITPAEIPGVADALYGEGFRILEVPLNS
                     PQPFDSIRLLADGYGDKCLVGAGTVIEIADVARVADAGGRLIVMPHADVAVVREAKRL
                     GMVCLPGVATPTEAFAALAAGADGLKLFPAEAMMPAALRAWRAVLPKSVLVFAVGGIR
                     PDNMAPWWAEGAAGF"
     gene            complement(557..1486)
                     /locus_tag="VFO53_01430"
     CDS             complement(557..1486)
                     /locus_tag="VFO53_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HET8874963.1"
                     /translation="MSATALVAIDWGTTSARAYRLDARGEVLARRSVPLGVKHVRDGR
                     FEPALAKLLGEWIDDTAPRIACGMIGSRQGWVEAPYVDCPASLTALSDRIVHAGNDVL
                     AIVPGVTTRDAAGVPDVMRGEETQLLGAIGRRERNVLAVLPGTHSKWARVEHGRVVDF
                     TTFLTGELYAVLIDHSMLGRLAGHEPGKFSRDVFSRGAARGLADGELSHDIFGARTLA
                     LTGALESEHVADWLSGLLIGREVRAARAWGLRAGIEPATVRVIGTDTLVERYELALAD
                     AGLVTERGHADAATRGLWRIAVHAGIVAAPVDA"
     gene            complement(1504..1950)
                     /gene="moaE"
                     /locus_tag="VFO53_01435"
     CDS             complement(1504..1950)
                     /gene="moaE"
                     /locus_tag="VFO53_01435"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HET8874964.1"
                     /translation="MTARVQTGDFDIGAEIARLRAADRGVGAIASFVGTVRDVNDAAT
                     ITGMTLEHYPGMTEAALDEIIATARQRFDIRDALVIHRVGTLEPGDQIVLVVVTSAHR
                     GTAFDACEFIIDWLKTRAPFWKKERLPDGERWVEARASDDEAAQRW"
     gene            complement(1961..2242)
                     /locus_tag="VFO53_01440"
     CDS             complement(1961..2242)
                     /locus_tag="VFO53_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET8874965.1"
                     /translation="MPDTLVTTRLRIRVVYLARLREALHSAGEIFELPADATVDSLVD
                     ALRARGGAFAAELAEGRAIRFAVNHRIVARSQAIADGDEVAIFPPVTGG"
     gene            complement(2314..2994)
                     /locus_tag="VFO53_01445"
     CDS             complement(2314..2994)
                     /locus_tag="VFO53_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET8874966.1"
                     /translation="MNAKPILVVTALAALVASAPGLAQEKLASAVDTGGFYGGVALRS
                     SGLDEGGVKFGHLTSAWGHFSSPVSDDTTNRALLYGGYRFANDIAVEGSLATADRYAL
                     RPVGAAGPGGIGLTLSSTVAGMPARTWNADVYTSWSFRKAFALYGRLGYAQSDNVPAY
                     ALATLPVSDARRLRDGMNYGVGVRYDFSSALGLRLEYARFSRFAGEAVTGPLPESDQV
                     QLGMQFRF"
     gene            complement(3107..3619)
                     /gene="mobB"
                     /locus_tag="VFO53_01450"
     CDS             complement(3107..3619)
                     /gene="mobB"
                     /locus_tag="VFO53_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HET8874967.1"
                     /translation="MRVFGFAGWSGSGKTTLIEAVIPKLTAQGLTVSLVKHAHHAFDI
                     DHPGKDSYRHRAAGCTEVLISSGARWALMHELRAGPELSLFDALARLSPCDIVLVEGY
                     KRAPIPKLEVWRAVLGKPLLNPHDPHIVAVAADPVGGAGGALPELPLSDAAAVATFIA
                     GAAAAWRSGD"
     gene            complement(3631..4806)
                     /locus_tag="VFO53_01455"
     CDS             complement(3631..4806)
                     /locus_tag="VFO53_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET8874968.1"
                     /translation="MRIGVPRETKDGERRVGIVPAGVSALARTGHTILVERDAGHGSG
                     FSDDDYASAGAKIVADAVDVWQSTLIVKVKEVQRAEIPLLRPATTIFGFAQINRDPTL
                     LDALLESGVRVVAYETVRDEAGALPLLAPMSRIAGRLAPLVGGEALQTDRGGNGTLLT
                     GVDGVEAARVVVIGAGSVGTEAARIAARLGCRVVVLSRGQRRLDALARTLAAEQLCVT
                     TTQLGSASSQLDSEIAAADLVIGAVLEPGTLSPKLISRAALRGMRRGSAFVDVGIDQG
                     GIAETSRMTTLSDPTYVDEGIVHYAAPNLPALVARTATFALAAATLPYVTALADAGIA
                     DALDRDAGLAAGTMVWDGAITHGGLATDAGRRQVVAPWRRQSARIHESAVGRIAGKP"
     gene            4982..5599
                     /locus_tag="VFO53_01460"
     CDS             4982..5599
                     /locus_tag="VFO53_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874969.1"
                     /translation="MENPLVPRRTATSDQDRHGPIIDVLERVLADIPASTAKPNDAPE
                     SAAKKIARQAAKRAAMLSGTLALPPGPLGFMTLLPDLYLIWQTQRQMVADIFGVYGRS
                     AELTRTHMLYCLFRHAASHVLRDVVVRGGQRMVIRQLSGGALRSAVGSIGVTISQRIA
                     GNATARWIPLAGAAAVGAYAYWDTLQVANTAQRLLSAPVEAIVDD"
BASE COUNT          986 a         1964 c         1885 g          887 t
ORIGIN      
        1 cgaaacccgc ggcgccttcg gcccaccacg gcgccatgtt gtcgggacga atgcccccaa
       61 cggcgaacac gagcaccgac ttcggcagca ccgcgcgcca cgcacgaagc gccgccggca
      121 tcatcgcttc ggcaggaaac agcttgagac catcggcacc ggccgcgagt gctgcgaatg
      181 cttccgtcgg cgtcgcgacg ccgggcaggc aaaccatgcc cagccgcttc gcctcgcgaa
      241 ccacggcgac gtcggcatgc ggcatcacga tgagccggcc gcctgcgtca gcaacgcgcg
      301 cgacgtcagc gatctcgatc accgtgcccg cccccacgag gcacttgtcg ccatatccat
      361 cggcgagcaa gcgaatcgaa tcgaacggtt gcggcgaatt gagcggcacc tcgaggattc
      421 gaaagccttc gccgtacaag gcatcggcga cgccgggaat ctcggcgggc gtgattccgc
      481 gcaataccgc gacgagtggc aacggttgca ggtaggcgtc gagcatcatt ggcgaatgcg
      541 aaggttcgaa atgagatcag gcgtcgaccg gcgctgcgac gatgccggcg tgaaccgcga
      601 tgcgccagag cccacgcgtc gccgcatcgg cgtgcccgcg ctcggtaacg agacctgcgt
      661 ccgcgagtgc caactcgtag cgctcgacga gcgtgtcggt gccgatgacg cgaaccgtcg
      721 caggctcgat gccagcgcgc aagccccatg cacgtgctgc gcgcacctcg cgaccaatca
      781 acaggcccga cagccaatcg gcaacgtgtt cggactcgag cgcgcccgtc agcgccagcg
      841 tgcgcgcgcc gaagatgtcg tgagaaagct cgccgtcggc gagaccgcga gccgcaccgc
      901 gcgagaacac gtcacgcgag aattttcccg gctcgtgccc cgcaagacgg ccgagcatgc
      961 tgtgatcgat cagcaccgca tagagctcgc ccgtcaggaa cgtcgtgaaa tcgacgacgc
     1021 ggccatgctc gacgcgcgcc catttgctgt gcgttcccgg caacacggcg aggacgttgc
     1081 gctcgcgccg gccgatcgcg cccagcaact gcgtttcctc gccgcgcatc acatcgggaa
     1141 cgccggccgc atcgcgcgtc gtaacgccgg gaacgatcgc gagcacgtcg tttcccgcat
     1201 gaacgatgcg atcggagagc gccgtcagcg acgccggaca gtcgacatac ggcgcctcga
     1261 cccatccctg ccggctgccg atcattccgc atgcgatacg cggcgccgtg tcgtcgatcc
     1321 attcgccgag cagcttcgcg agcgccggct cgaagcgtcc gtcgcggaca tgcttgacgc
     1381 cgagcggcac gctgcggcgc gccagcactt cgccgcgcgc atcgaggcgg tacgcgcgcg
     1441 cagaggtcgt tccccagtcg attgcgacga gcgcggtggc ggacattcgc gggctgccgc
     1501 tcgtcaccag cgctgcgcgg cttcgtcgtc gctcgcgcgc gcctcgaccc agcgctcgcc
     1561 atcgggcagc cgttcctttt tccagaacgg cgcgcgcgtc ttcagccagt cgatgatgaa
     1621 ttcgcacgcg tcgaacgcgg tgccgcggtg agcactggtc accacgacga gcacgatctg
     1681 atcgccaggc tcgagcgtgc ctacgcggtg gatgacgagc gcgtcgcgaa tatcgaaccg
     1741 ttgccgcgcc gtagcgatga tctcgtcgag cgcggcctcg gtcatccccg ggtaatgctc
     1801 gagcgtcatg ccggtgatcg tcgcggcgtc gttgacgtcg cgcacggtgc cgacgaacga
     1861 ggcgatcgcg ccgacgccgc ggtcagccgc ccgcagccgc gcgatctcgg ccccgatgtc
     1921 gaaatcgccg gtctgcacgc gagcggtcat aacgcgcgac tcagccaccg gtgaccggcg
     1981 gaaagatcgc gacctcgtcg ccgtcggcaa tcgcttgcga acgcgcgacg atacggtgat
     2041 tgacggcgaa ccgaatcgcg cgcccttctg cgagctccgc cgcaaaggcg ccgccgcgtg
     2101 cgcgcaaggc gtcgaccagc gaatcgacgg tcgcgtccgc aggcagctcg aagatttctc
     2161 cggcgctgtg aagcgcctca cgcagccgcg ccagatagac aacgcgaatg cgaagccgtg
     2221 ttgtaacgag ggtatcgggc atccggcgtt cgaatgaaaa agcgccggac ttcgcttaag
     2281 aagaccggcg ctcgattgta cgacgtctct tcgtcagaac cggaactgca tccccagctg
     2341 gacctgatcg gattcgggca acggtcctgt gaccgcttcg cccgcgaacc ggctgaaacg
     2401 cgcgtattcg agtcgcagac ccagcgcgga tgagaaatcg tagcgcacgc caacgccgta
     2461 gttcatgccg tcacgcaacc gtcgcgcatc gctgacgggc aatgttgcca gcgcatacgc
     2521 gggcacgttg tcgctttgcg cgtagccgag gcggccatac agcgcgaatg ccttgcggaa
     2581 cgaccagctc gtatagacgt cggcgttcca cgtgcgcgcg ggcatgccgg caacggttga
     2641 ggacagtgtc aggccgatgc ctcccgggcc tgcggcgccg accggccgga gcgcgtagcg
     2701 atcggcagtg gcgagcgagc cttcgacggc gatgtcgttt gcaaaccggt agccgccgta
     2761 caggagcgcc cggttcgtcg tgtcgtcact gacgggcgag gagaaatgtc cccacgccga
     2821 tgtgaggtgg ccgaatttga cgccgccttc atcgagccct gagctgcgca gcgcaacgcc
     2881 gccataaaag ccgccggtgt cgacggcgga tgccaacttc tcctgcgcga gccccggtgc
     2941 tgacgcgacg agcgcggcca gcgcggtgac gacgaggatg ggcttggcgt tcatggctgg
     3001 aactcgttga taaggcgcac ttttttgcgc tgcggcacac gcggccgata tcgcttggac
     3061 agccgccgta ccgacagttc aaaactaacc cgtcagacgc ttttcgtcaa tcgccggacc
     3121 gccaggcggc agcggctccg gcaatgaatg tggcgacggc cgcagcgtcc gacaacggca
     3181 gctcgggcag cgcgcccccg gccccgccca ccggatcggc ggccacggcg acgatgtgcg
     3241 gatcgtgagg gttcagaagc ggcttgccga ggaccgcgcg ccacacttcg agtttgggga
     3301 tcggcgcacg cttgtacccc tcgacgagga cgatgtcgca cggcgagagc cgcgccagcg
     3361 catcgaacag cgacagctcg ggaccggccc tcagctcgtg catcagcgcc caccgcgcac
     3421 ccgagctgat cagcacctca gtgcaacccg ccgcgcggtg acggtacgaa tccttgcccg
     3481 gatggtcgat gtcgaacgcg tgatgcgcgt gcttgacgag tgacaccgtg aggccttgcg
     3541 ccgtcagctt cggtatcacc gcctcgatca gcgtcgtctt gccgctgccc gaccagcccg
     3601 cgaagccgaa gacgcgcatc tccggggtgt tcatggcttg cctgcgatgc gtcccacggc
     3661 gctttcgtga atgcgggccg attgacgacg ccacggcgca acgacctgcc gccgccccgc
     3721 atcggtggcg agaccgccat gcgtgatggc gccgtcccag accatcgtgc cggcggcgag
     3781 gcctgcgtcg cggtcgagcg cgtcggcgat gccggcatcg gcgagcgccg tcacgtaagg
     3841 cagcgttgcc gcagcgagtg cgaacgtagc ggtccgcgcg acgagtgctg gcagattcgg
     3901 cgcggcgtag tgaacgatgc cctcgtcgac gtacgtcggg tcggacagcg tcgtcatccg
     3961 cgacgtctcg gcgatgcctc cctgatcgat gccgacgtcg acgaaggccg atccacgacg
     4021 catgccgcga agcgctgcgc gcgagatgag cttcggcgac agcgtccccg gctcgagcac
     4081 cgcaccgatc acaagatcgg cggccgcgat ttcggaatcg agctggctcg atgctgaacc
     4141 gagctgcgtc gtcgtgacgc aaagttgctc ggcggcgagt gtgcgcgcga gggcgtcgag
     4201 ccggcgctgt ccgcgcgaca gcacgacgac gcggcagccg agcctcgccg caattcgcgc
     4261 tgcttcagtg ccgacgctgc cggcgccgat gacgacgacg cgcgccgcct cgacaccatc
     4321 aacacccgtc agcagcgtgc cgttaccgcc gcgatccgtc tgcagcgctt cgccgccaac
     4381 gagcggcgcc agtcgaccgg cgatgcgcga catcggcgcg agtagtggca acgcgccggc
     4441 ctcatcgcga acggtctcgt acgcgacgac gcgcacaccg ctttcgagca gcgcatcgag
     4501 gagcgtgggg tcgcgattga tttgcgcgaa cccgaaaatg gtcgtcgcgg gacgcagcaa
     4561 tggaatctcc gcgcgctgaa cttccttgac cttgacgatc agcgtggact gccagacgtc
     4621 cacggcgtcc gcgacgattt ttgccccggc agaagcgtaa tcatcgtcgg agaatccgct
     4681 gccgtgcccg gcatcgcgct cgacgaggat cgtgtgtccc gtgcgtgcga gcgcgctgac
     4741 accggccgga acgatgccga cgcggcgctc gccatccttc gtctcgcggg gtacgccgat
     4801 gcgcactggt gggcctccgt ataacgaacg atgttagtga ctgcgacggc gcaattgtag
     4861 tgcgcggcaa cttcaatccg ctgaccgcgc gctctcgcgt cgttgcatgc cgctcgccat
     4921 cgcggggcgc gcaacgactc tgccgatgta gactcgcatc atcgtcccca tatgacgagc
     4981 catggaaaat ccgctcgttc cccgccgcac cgcgacgtcc gaccaggatc gtcacggacc
     5041 gatcatcgat gtgctcgagc gcgtgctcgc ggacattccc gcgtcgacgg cgaagccgaa
     5101 cgacgcgccc gaaagcgccg cgaagaaaat cgcccgccag gcagcgaagc gtgcggcgat
     5161 gctgtcggga acgctcgcgc ttccgccggg gccgctcggc ttcatgacac tgcttcccga
     5221 cctgtacctg atctggcaga cgcagcgcca gatggtcgcc gacatcttcg gcgtttatgg
     5281 ccgcagcgcc gagctgacgc gcacgcacat gctgtattgc ctgtttcgcc atgcggcgag
     5341 tcacgtgctg cgcgacgtcg tcgttcgtgg cggacagcga atggtcattc ggcaattgag
     5401 cggtggcgca cttcgcagtg cggtcggcag catcggcgtc acgatctccc agcggattgc
     5461 gggcaacgca acggcgcgct ggattccgct cgctggcgcc gcggccgtcg gtgcgtacgc
     5521 gtactgggac acgctgcagg tggcgaacac ggcgcagcgc ctcttgagcg cgcccgtcga
     5581 agcgatcgtc gacgattgat tgcgccgatt cgcgttttcg accgcagcgt caacgcgcgt
     5641 taacgtcgaa tgtgcggccg ctacgagttg tcgagccatc cggcggcgat tgcactggcg
     5701 ttcggcttgg tgcacccgcc cg
//
LOCUS       DASSVX010000030        12839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_234536, whole genome shotgun sequence.
ACCESSION   DASSVX010000030 DASSVX010000000
VERSION     DASSVX010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12839
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234536"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFO53_01465"
     CDS             complement(<1..401)
                     /locus_tag="VFO53_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET8874970.1"
                     /translation="MTLSMHARDAGRRAMSGTSRCKAIATAVALLALAGCSSLERSRD
                     VGDPLVDGRTLAMQVCSNCHGIDGDSISPNFPRLAGQPSEYLAAQLKSFRAKDRRDPA
                     GFEYMWGLSRKLTDPQIDAIAAYFHAQAPRA"
     gene            complement(428..1864)
                     /locus_tag="VFO53_01470"
     CDS             complement(428..1864)
                     /locus_tag="VFO53_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HET8874971.1"
                     /translation="MSDRTQASHPRTRQSHPSCRSRPAHRLILALIGAIVTAAFANGA
                     LAVPAFNRQTGQNCVACHAGGQFPELTAYGRLFKLTGYTLGSRDVPLSLMAVAGYTRT
                     EHTAGQDPSNFPKNGAPAFQGGSVFVAGKVTDNLGVFAQITYDNYAQQNASGEFVGHS
                     GSDNMDFRYADRLIDSRRDLIFGATVNNNPTVQDPWNSTPAWAYPYVQSSFQVSPSTN
                     GPLLTGDLAQQAAGAGAYLFWNKTLYAELSLYRTANGFWSFMSQGTQNDDQTKLKGAN
                     PYWRFALSHEWGAHSAMVGVFGLEARVYPDNEDPTGDTSRYRDVGVDAQYQYLLDPHA
                     ITVTASYIHERTRWAPEIVAEGESSNASDTLYQLKVKGSYVYQARYGASLTYFRIGGS
                     TDAALYGGGVDDSGMPLGSPVSGNLAGSPGTSGVVAEIFWTPVQWVRVGAQYWAYTRF
                     NGASSNYDGFGRNARDNNTLFVYVWGAY"
     gene            complement(1992..2900)
                     /locus_tag="VFO53_01475"
     CDS             complement(1992..2900)
                     /locus_tag="VFO53_01475"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET8874972.1"
                     /translation="MPRPPRTSARQGTPAVASERPRYTTLIGTAELAHHLDDAAFVVV
                     DVRHDLANPAYGENAYAQAHLPGAVFAHVDGDLSAAATGRNGRHPLPAPEAAAMVFGR
                     LGIDAAKQVVTYDQGSGIYAARLWWMLRWLGHDNVAVLDGGFAKWSREGRPLESEARA
                     ATPTQFVPARVRPTVNATGIAASLPRHDLVLLDARAAERYRGDVEPLDPVAGHIPGAL
                     NRPHSRNVAADGTFRSAGELRGEFDAMLHGRSPGDVVHYCGSGVSACHNILAMTIAGY
                     PLARLYPGSWSEWCADPKRPVARGQV"
     gene            complement(2908..4017)
                     /locus_tag="VFO53_01480"
     CDS             complement(2908..4017)
                     /locus_tag="VFO53_01480"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET8874973.1"
                     /translation="MSDLATAARLRQATSQLPAHWYCDPRVFHAERTQLLARAPGYVG
                     HALMTPEPGDYHVLAARDNAQALVRGPQGVELLSNICRHRQAIMLTGRGNARNIVCPI
                     HRWTYDLQGKLLGAPHFENQPCAQLGRTPLSNWHGLLFDGPRDVARDLAGLTLKDIDF
                     TGYVLNRVDLHPCNYNWKTFIEVYLEDYHVEPFHPGLGQFVTCNDLRWEFAERASVQS
                     VGVNSGLAKPGTRAYERWHKAVLDYYRGQQPAQGAIWLTYYPNIMVEWYPHVLVISTL
                     VPQAVDRTLNVVEFYYPEDIALFEPEFVEAEQAAYHETAKEDDEIAERMDAGRKALYA
                     QGRSEVGPYQSPMEDGMQHFHEFYRREMEVHLAGL"
     gene            4241..4480
                     /locus_tag="VFO53_01485"
     CDS             4241..4480
                     /locus_tag="VFO53_01485"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET8874974.1"
                     /translation="MSETAESPPSFEAALAELEALVAQMESGELPLAESLAAYKRGAA
                     LLAHCQAALKDAQQQVEVLEKGVLQPFAAGEPDDA"
     gene            4473..5354
                     /locus_tag="VFO53_01490"
     CDS             4473..5354
                     /locus_tag="VFO53_01490"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HET8874975.1"
                     /translation="MPDASFAAWSRERQQRIEGVLDRVLPGADAVAARLASAMRYATL
                     GGGKRMRPLLVYAASEVTHADAAAADVCAAAVELIHAYSLIHDDLPCMDDDSLRRGKP
                     TCHVAFDVATALLAGDALQSLAFAVLAGCPGNDAQACRVLADAAGLFGMAGGQQLDLD
                     ATGTALGADSLAQLHMMKTGALIRASVRLGARCGRTMTSDQAGALDRYAAAAGLAFQV
                     VDDVLDVEGSSATLGKTAGKDAAQHKATFVTLLGLDGAKARAESLREEAHAQIAPLGA
                     DARRLAELADWIVLRNR"
     gene            5373..7262
                     /gene="dxs"
                     /locus_tag="VFO53_01495"
     CDS             5373..7262
                     /gene="dxs"
                     /locus_tag="VFO53_01495"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET8874976.1"
                     /translation="MDTYPLLERIESPADLRRLDRSDLSQLVRELRAFLLRSVSQTGG
                     HLSSNLGTVELTVALHYAFDTPRDRIVWDVGHQTYAHKILTGRRRRMDKLRQWGGPGG
                     FPRRDESEYDTFGTAHSSTSISAALGMAAAAKLNGDPRSIVAVIGDGAMSAGMAFEAL
                     NNAEGANVLVILNDNDMSISEPVGAFNNYLAKVLASRFYNSVRRGGKEVLAKLPPVHE
                     LARRAEVHVKGMVLPGTLFEEFGFNYIGPIDGHDVDLLVRTLMNVRKLNGPQFLHVVT
                     RKGYGYARAEEDPILYHGVTKFDPAVGIVPKTGGKPTYTQVFGDWLCDMARADRRLVG
                     ITPAMREGSGLVEFSQRFPERYYDVGIAEQHAVTFAAGLACEGMKPVVAIYSTFLQRA
                     YDQLIHDVALQNLPVMFAIDRAGIVGADGPTHTGAFDLTYLRCLPNTPVMAPADENET
                     RQMLTTAFHSSTPSAVRYPRGTGPGVPIAAALGTLPIGKGEMRRTAKRRANRIAILAF
                     GPLLYPALAAAEELDTSVANMRFVKPLDTELIRALARDHDAFVTVEENVVAGGAGSAV
                     AEFLASEKLALPILHLGLPDRFIDHGDPAFLLAHVGLDAKGIAASIHSRFAQRTPESW
                     AKPAA"
     gene            7351..8169
                     /gene="folE2"
                     /locus_tag="VFO53_01500"
     CDS             7351..8169
                     /gene="folE2"
                     /locus_tag="VFO53_01500"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HET8874977.1"
                     /translation="MNRPETPNHLLSIPDVQSSRDERRLAIDRVGIKGLRYPLPFADG
                     DGEARTTIATCAVYVALPEEQKGTHMSRLVSLLEERASPGAAPLSVAALRGFVEELVA
                     RLGAPGGRLEIEFPFFVRKIAPVSGVASLLDYDVRLTAELAGNVYTSTISVSAPVTSL
                     CPCSKEISEYGAHNQRSLVTIAVRPRAPVRIAELLRIGEDEASCELYGILKRADEKYV
                     TEHAYDNPRFVEDLVRGVASRLAADDRFTAFTVEAENFESIHNHSAYARIARGW"
     gene            complement(8173..10092)
                     /locus_tag="VFO53_01505"
     CDS             complement(8173..10092)
                     /locus_tag="VFO53_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HET8874978.1"
                     /translation="MDTPHPAIAVPGIAAPSRTREGSAALALLALGVVYGDIGTSPLY
                     AAKETFNPQHGIPLTPENVIGGVSAIFWALMIIVSLKYVTLVLRASNRGEGGIMALLA
                     LATSSVRAHPRLRAALLVIGVFGASLFYGDAVLTPAISVLSAVEGLEVGTDALAPYVV
                     PIATVILIALFAIQRFGTGFVGRLFGPVCAIWFVSLACAGAWNIGKAPVILTALDPVH
                     ALRFTTQHGSASFVVLGAVLLAVTGAEALYADMGHFGKRAIRIAWFGVAAPALVLDYF
                     GQGALLIATPTALANPFYLSYPAWALYPMIVLATAATVIASQATISGAYSMTKQAVQL
                     GYLPRLSIVHTSPRTIGQIYVPAVNWTLLVMVAAAVVGFGSSSRLASAYGVAVMGTML
                     VTTLLTFFVLRYAWHYPGWLCTLATAFFMFVDTAFFAAAMNKVRDGGWFPLALGGVVF
                     FVMLTWRRGRALLLERLRGGSPPLAGLLAALFADPPQRVPGTAVFLTSSPDATPNALL
                     HSLKHYKVLHARNVFLTVEFADVPWMPDAEAVRCEPLANDCWRVVARYGFMERPDVAL
                     ALEVCAPQGLSIEPMEASYFLSREKIVPGTSERGLARLRDRLFAAMARNAGSITDFFN
                     IPPNRVVELGTRVEL"
     gene            10242..11309
                     /locus_tag="VFO53_01510"
     CDS             10242..11309
                     /locus_tag="VFO53_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HET8874979.1"
                     /translation="MKTQPRLAGYGAAVLAATAGTLAGWAMRPRFDIVNIAMVYLLAV
                     VIVSLRFSRGAAIVTSILCVAAFDYLFVPPRGTFTVADIQYLLTFAIMLAVALVISHL
                     VDSVRRQAARQASLTVDAETERIRSALLASISHDLRTPLAVLAGASSSLASSGDRLSA
                     VEREALATSVVRQAREMSEHVEKILQMTRLEAGAIRLDRDWASLGEIVGSVLLRLGER
                     LAGHRIIVEIPGDLPLVRVDATLIEQALMNLLDNCARHTPADTVVRLRAKARAADIVV
                     TVEDYSGGLSDHDFERLFAKFHHAAAEGSGSGVGLGLAICRAIVRLHDGQAWAERVPG
                     GGTAFRFTLPREPSPAAPAEP"
     gene            11311..12006
                     /locus_tag="VFO53_01515"
     CDS             11311..12006
                     /locus_tag="VFO53_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8874980.1"
                     /translation="MTDPRPTVLIVEDEPEIRRFLRATLAANDYRVVESATGRRGQID
                     AGTHKPDLAIVDLGLPDVDGIDVIRRIREWSPMPVIVLSARIQERAKIDALDAGADDY
                     VTKPFAVGELLARVRVALRHAVRGAAVTSTLRIGAACVDLERRTARRGEDDVRLTPIE
                     FRLLATLARHLGLVVTHRQLLTEVWGPTHASDTHYLRIYMKQLRDKLEPDPLRPRHLI
                     TETGIGYRLLGDE"
     gene            12140..>12839
                     /locus_tag="VFO53_01520"
     CDS             12140..>12839
                     /locus_tag="VFO53_01520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET8874981.1"
                     /translation="MRPIAVFRFSPTEGPAHFAQWLDAAALPWQLIAVDEGDAIPADA
                     TQFAGIGMMGGPMSVNDVLAWIDPLSRLLRHAIARDIPVIGHCLGGQLLAQALGARVR
                     RTQTPEIGWIDVESTDAAARDAWFGGRAHFTPFQWHYDVFELPPGASRVLTNSFNPNQ
                     AYVIGEKHIGFQCHIEMTADLVETWCRSGADEIAAPGTAARQSRDEILRDLHARLDAL
                     SIVADGVYARWSQGL"
BASE COUNT         2112 a         4322 c         4329 g         2076 t
ORIGIN      
        1 ttcgcccttg gtgcctgcgc gtggaaatag gccgcgatgg cgtcgatttg cggatcggtc
       61 agcttgcggc tcaggcccca catgtattcg aatccggcgg gatcgcggcg atccttggcg
      121 cgaaacgact tgagttgcgc cgcgaggtac tcggaaggtt gcccggcaag gcgcgggaag
      181 ttgggcgaga tcgaatcgcc atcgatgccg tggcaattcg agcagacctg catggcaagc
      241 gtgcgtccgt ccaccagtgg atcgcccacg tcgcgggaac gctcgagtga cgagcagccg
      301 gcaagcgcca acagcgcgac ggctgtcgcg atcgccttgc atcgactcgt cccagacatc
      361 gcgcggcgcc cggcgtcgcg cgcatgcatc gacagtgtca cgatttcctc cgctttcgcg
      421 cgtgcgatca gtaggcgccc cagacataaa cgaacagcgt gttgttgtcg cgggcattgc
      481 ggccgaaacc gtcgtaattg ctggaggcgc cgttgaaccg cgtgtacgcc cagtactgcg
      541 ctccgacccg aacccactgc accggcgtcc agaagatctc cgcgacgacg ccactcgtcc
      601 ccggcgagcc cgcgaggttg ccgctgacgg gcgaacccag cggcatcccg ctgtcgtcga
      661 cgccaccgcc gtagagcgcg gcatcggtcg atccgccgat gcgaaaatac gtaaggctcg
      721 cgccgtagcg ggcctggtac acgtagctcc ctttcacctt gagctgatac agcgtgtcgc
      781 tcgcattgga gctctcgcct tcggcgacga tctccggagc ccatcgcgtg cgctcgtgga
      841 tgtagctcgc cgtgacggtg atcgcgtgcg gatcgagcag gtactggtat tgcgcatcga
      901 cgccgacgtc gcgatatcgg ctcgtatcgc cggtcggatc ctcgttgtcg ggatagacgc
      961 gagcttcaag gccgaacacg ccgaccatgg cgctgtgcgc gccccattcg tgcgacagcg
     1021 cgaagcgcca gtaagggttt gcgcctttga gcttggtctg gtcgtcgttc tgcgtgccct
     1081 ggctcatgaa cgaccagaat ccgtttgccg tccggtaaag cgacaattcg gcgtagagcg
     1141 tcttgttcca gaacaggtag gcgccggcgc cggcggcctg ttgcgcaagg tcgcctgtca
     1201 acaacgggcc gttcgtggac ggcgagacct ggaacgagct ctggacgtag ggataggccc
     1261 aggcgggcgt actgttccac ggatcctgca cggtcggatt gttgttcacc gtcgcgccga
     1321 agataaggtc gcgccgtgaa tcgatcaggc gatccgcata gcggaaatcc atgttgtcgg
     1381 agcccgagtg ccccacgaac tcgccggaag cattctgctg cgcgtagttg tcgtaggtga
     1441 tctgcgcgaa tacgccgagg ttgtccgtca ccttgccggc aacgaacacg ctgccgccct
     1501 ggaacgccgg cgcgccgttc ttcgggaaat tcgacgggtc ctggccggcc gtgtgctcgg
     1561 tgcgcgtata tccggccacc gccatcagcg acagcggaac gtcgcgcgat ccgagcgtgt
     1621 agccggtgag cttgaacagc cggccgtacg cggtgagttc aggaaactgg ccgcccgcat
     1681 gacaggcaac gcagttctgc ccggtctggc gattgaatgc gggtacggca agggcaccgt
     1741 tcgcgaaagc ggcggtgacg atcgcgccga tcagcgcgag tatcagccgg tgcgcaggtc
     1801 gcgatcggca tgacggatgc gactgcctcg tacgtggatg cgacgcttgc gtccgatccg
     1861 acatcgcggc ctccgccgat gtcgccggta ctgatgaaca ccggcccgac gaatccagcc
     1921 tagcgcaacg cgatcggcgg cggattgacg cggatcaacg ttcgacgcga tttcgccgat
     1981 cgtgatgaac cctacacttg tccgcgcgcc accggccgct tcggatcggc gcaccactcg
     2041 ctccacgagc ctggatagag acgcgcgagc gggtagccgg cgatcgtcat cgcgaggatg
     2101 ttgtggcatg cgctgacgcc cgagccgcag taatgcacga cgtcgcccgg cgagcggccg
     2161 tgcagcatcg cgtcgaactc gccgcgcagt tcgcccgccg agcgaaacgt gccgtcggcc
     2221 gcgacgttcc gactgtgcgg tcgattgagc gcgccgggaa tgtgaccggc aacgggatcg
     2281 agcggctcga cgtcgccgcg gtagcgctcg gcggcgcgtg cgtcgagcag cacgagatcg
     2341 tggcgcggca ggcttgccgc gatgcctgtc gcgtttaccg tcggccgcac acgagcgggc
     2401 acgaattgcg tcggcgtcgc cgcacgcgct tcgctttcca gaggacgccc ttcgcgcgac
     2461 cacttcgcaa agccgccgtc gagcacggcg acgttgtcgt gaccgagcca gcgcagcatc
     2521 caccaaaggc gcgccgcgta aatgccgctg ccctggtcgt aagtgacgac ctgcttcgcc
     2581 gcatcgatgc cgaggcggcc gaaaaccatc gcggccgcct ccggcgctgg caacggatgg
     2641 cggccgttac ggcccgtggc cgctgcggac aggtcaccgt cgacgtgcgc gaacacggca
     2701 cccggcaggt gcgcctgcgc atacgcgttt tcaccgtacg cgggattcgc gagatcgtga
     2761 cgcacgtcga cgacgacgaa cgccgcatcg tcgagatggt gcgcaagctc cgccgtgccg
     2821 atcagcgtcg tgtaacgcgg gcgctcgctc gcgaccgcgg gggtgccttg acgcgcggaa
     2881 gtgcgggggg gccgtggcat cacgacgtca gagaccggcg agatggactt ccatctcgcg
     2941 ccggtagaac tcatggaaat gctgcatgcc gtcttccatc ggcgactggt acggaccgac
     3001 ctcgctgcgt ccctgcgcgt acagcgcctt gcggcccgca tccatccgtt cggcgatctc
     3061 gtcgtcctcc ttcgccgttt catggtacgc cgcctgctcg gcctcgacga attcgggctc
     3121 gaacagcgcg atgtcctcgg gatagtagaa ctcgacgaca ttgagcgtgc ggtcgaccgc
     3181 ctgcggcacc agcgtgctga tgacgagcac atgcgggtac cactcgacca tgatgttcgg
     3241 gtagtacgtg agccagatcg ccccttgcgc cggctgctgc ccgcggtagt aatcgagcac
     3301 cgccttgtgc cagcgctcgt aggcgcgcgt cccgggcttg gccaggccgc tgttgacgcc
     3361 gaccgactgc accgaagcgc gctcggcgaa ctcccagcgc aggtcattgc aggtgacgaa
     3421 ctggccgagg ccggggtgga acggttcgac gtggtaatcc tcgaggtaga cctcgatgaa
     3481 cgtcttccag ttgtagttgc acggatgcag atcgacgcgg ttcaggacgt aaccggtgaa
     3541 gtcgatgtcc ttcagcgtga gcccggcaag gtcgcgcgca acatcgcgcg gaccgtcgaa
     3601 gagcagtccg tgccagttcg agagcggcgt gcggccaagc tgcgcgcacg gctgattctc
     3661 gaagtgcggc gcgccgagca gcttgccctg gaggtcgtag gtccagcgat ggatcgggca
     3721 cacgatgttg cgcgcattgc cgcggccggt cagcatgatg gcctggcggt gacggcagat
     3781 gttcgacagg agctcgacac cctgcgggcc gcggacgagc gcctgcgcgt tgtcgcgcgc
     3841 ggccagcacg tgataatcgc cgggttcggg cgtcatcaaa gcgtggccga catagcccgg
     3901 agcgcgcgcg agaagctgcg tgcgctcggc gtggaaaacg cgcgggtcgc agtaccagtg
     3961 cgcgggaagc tgcgatgtgg cctgccgcaa gcgcgcggca gtggcgagat cggacatcga
     4021 tcatcctttc atgcgtgaat accaacccaa cccaacaacc cgggctgctc cggcattgcg
     4081 ccgatctgtc ccggaccgct ttgctaagtc gaccgcgcga aaggttcgcg taagacgagg
     4141 caaatcccgt cttaaaaagg ccgcgtcgcc aaaacatgct agtatattgg gctccccggc
     4201 cgcgggtcaa ggacctgatc ggtagcaaag cgcagcaccc atgtccgaga cagccgaatc
     4261 gccccccagt ttcgaagccg cgctcgccga gctcgaagcg ctcgtcgcgc agatggaaag
     4321 cggcgagctg ccgctggccg aatcgcttgc ggcttacaag cgcggcgccg cgctgcttgc
     4381 gcactgccag gcggcgctca aggatgcgca acagcaggtc gaggtgcttg aaaagggcgt
     4441 gctgcagcca ttcgcggccg gcgagcccga cgatgcctga cgcttcgttc gccgcctggt
     4501 cgcgcgagcg ccagcagcgt atcgaaggcg tgctcgaccg cgtgctcccc ggagccgatg
     4561 ccgtcgcggc gcggctcgcc agcgcgatgc gctatgcaac gcttgggggc ggcaagcgca
     4621 tgcgcccgct tctcgtctac gccgcgtccg aggtcacgca cgcggatgcc gccgccgccg
     4681 acgtctgcgc cgccgccgtc gagctgatcc atgcctattc gctgatccac gacgatctgc
     4741 cttgcatgga cgacgattcg ctgcgtaggg gcaagcccac gtgccatgtc gcgttcgacg
     4801 ttgccaccgc gctcctcgcc ggcgacgcgc tgcagtcgct cgcgttcgcc gtgctcgccg
     4861 ggtgcccggg caacgacgcg caagcgtgcc gcgtgctcgc cgacgccgcg ggacttttcg
     4921 ggatggcggg cggccagcag ctcgacctcg acgcgacggg aacggcactc ggcgccgatt
     4981 cgcttgccca attgcacatg atgaagacgg gggcgctgat ccgcgcgtcg gtgcgactgg
     5041 gcgcacgctg cggtcgtacc atgacgagcg accaggcggg tgcgctcgat cgctacgccg
     5101 ccgccgcggg cctcgcgttc caggtcgtcg acgacgtgct cgacgtcgag ggctccagtg
     5161 ccacgctcgg caagaccgcc ggcaaggacg ccgcgcagca caaggcgacg ttcgtcacgc
     5221 tgctcggtct cgacggtgcg aaggcgcgcg ccgagtcgct gcgcgaggag gcgcatgcgc
     5281 aaattgcgcc gctcggggcc gatgcgcgcc ggctggccga gctcgccgac tggatcgtgt
     5341 tgcgcaaccg ttgacggaac agtccccacg acatggacac ctatccgctt ctcgaacgca
     5401 tcgaatcgcc ggccgacctg cgccgcctcg atcgctccga cctttcgcag ctcgtgcgcg
     5461 agctgcgcgc gttcctgctg cgctcggtgt cgcagaccgg cggccacctg tcgtccaatc
     5521 tcggaaccgt cgagctcacc gtcgcgctgc attacgcatt cgacacgccg cgcgatcgca
     5581 tcgtgtggga cgtgggtcac cagacgtacg cccacaagat tctcaccggg cggcgccggc
     5641 gcatggacaa gctgcgccag tggggcggac ccggcggctt tccgcgccgc gatgaaagcg
     5701 aatacgacac gttcggcacc gcgcattcgt cgacgtcgat ctcggcggca ctcggcatgg
     5761 cggccgcggc gaagctcaat ggcgatccgc gcagcattgt cgcggtgatc ggcgacggcg
     5821 cgatgagcgc cggcatggcg ttcgaggcgc tgaacaacgc cgaaggcgcg aacgtgctgg
     5881 tgatcctcaa cgacaacgac atgtcgatct ccgagccggt cggcgcattc aacaactacc
     5941 tcgcgaaggt gcttgcgtcg cgcttctaca actcggtgcg ccgcggcggc aaggaagtgc
     6001 tcgccaagct gccgccggtg cacgagctcg cgcgccgcgc cgaggttcac gtcaagggca
     6061 tggtgctgcc cggcacgctg ttcgaggaat tcggcttcaa ctacatcggc ccgatcgacg
     6121 ggcacgacgt cgaccttctc gttcgcacgc tcatgaacgt gcgcaagctc aacggcccgc
     6181 agttcctgca cgtcgtcacg cgcaagggct acggctacgc gcgcgccgag gaggacccga
     6241 tcctctatca cggcgtcacc aaattcgacc cggccgtcgg catcgtgccg aaaaccggcg
     6301 gcaagccgac ctacacgcag gtgttcggcg actggctgtg cgacatggcg cgcgccgatc
     6361 ggcggctcgt cggcatcacg ccggcgatgc gcgagggctc gggtctcgtc gaattctcgc
     6421 agcgctttcc cgagcgctat tacgacgtcg gcattgccga gcagcatgcg gtcacgttcg
     6481 cggccggtct cgcctgcgag ggaatgaagc cggtcgtcgc gatctattcg acgttcctgc
     6541 agcgcgccta cgaccagctg atccacgacg tcgcgctgca gaaccttccg gtcatgttcg
     6601 cgatcgaccg cgccggcatc gtcggcgccg acggcccgac gcacaccggc gccttcgacc
     6661 tcacgtacct ccgctgcctg cccaacaccc cggtgatggc gcccgccgac gagaacgaga
     6721 cgcggcagat gctgaccacg gcgtttcatt cgagcacgcc ttcggccgtg cgctatccgc
     6781 ggggaacggg gcccggcgtt cccattgccg ccgcattggg cacgctgccg atcggcaagg
     6841 gcgaaatgcg ccgcacggcg aagcgccgcg cgaaccgcat cgccatcctc gccttcggtc
     6901 cgttgctgta tccggcgctc gctgccgcag aggagctcga cacgtcggtg gccaacatgc
     6961 gcttcgtgaa gccgctcgat accgaattga tccgcgcgct ggcgcgcgac cacgacgcgt
     7021 tcgtgaccgt cgaggaaaac gtcgtcgccg gcggggcggg cagcgccgtt gccgaattcc
     7081 tcgcctcgga gaagcttgcg ctgccgatcc tgcacctcgg tctgcccgac cgcttcatcg
     7141 atcacggcga cccggcgttc ctgctcgcgc acgtcggact cgacgccaag ggcatcgcgg
     7201 cgtcgatcca ctcgcgcttc gcgcaacgca cgccggaaag ctgggcgaag cccgctgcct
     7261 gaaacgcggg tgcccttaga ggcatccaat gggttcgagc gagcggccgc gtccggccgc
     7321 gcgagaccag tatggcaaac ttgaagcgcc atgaaccgac ctgagacgcc gaaccatttg
     7381 ctgtccattc ccgacgtgca gtcgagtcgc gacgagcgcc gccttgcgat cgaccgcgtc
     7441 ggcatcaagg gcctgcgcta tccgctgccg tttgccgacg gcgacggcga ggcgcgcacg
     7501 acgatcgcaa cctgcgcggt gtacgtggcg ttgcccgagg agcagaaggg aacgcacatg
     7561 tcgcggctcg tgtcgctgct cgaggagcgg gcgagcccgg gtgctgcgcc gttgtccgtg
     7621 gccgcattgc gcggcttcgt cgaggagctc gtcgcgcgcc tcggcgcgcc gggcggccgg
     7681 ctcgaaatcg aatttccgtt cttcgtgcgc aagatcgcgc cggtgtcggg cgtggcaagc
     7741 ctcctcgatt acgacgtgcg cctgacggcc gagctcgccg gcaacgtcta cacgagcacg
     7801 atcagcgtct cggcgccggt gacttcgctt tgcccctgct cgaaggaaat ctccgagtac
     7861 ggcgcacaca accagcgctc gctggtgacc atcgccgtgc gtccgcgcgc accggtgcgc
     7921 attgccgaat tgctgcgcat cggcgaggac gaggcgtcgt gcgagctcta cgggatactg
     7981 aagcgcgccg acgagaagta tgtcaccgag cacgcttacg acaatccgcg cttcgtcgag
     8041 gatctcgtgc gtggcgtcgc gtcgcgcctc gccgccgacg atcgcttcac ggcgttcacg
     8101 gtcgaggccg agaacttcga gtcgatccac aatcactcgg cctacgcccg catcgcgcgg
     8161 gggtggtagc gcctacagct cgacgcgggt tccgagctcg accacgcgat tgggcggaat
     8221 gttgaagaag tcggtgatgc tgcccgcgtt gcgcgccatt gccgcgaaca gcctgtcgcg
     8281 cagtcgcgcg agcccgcgct cggacgttcc gggcacgatc ttctcgcgcg acaggaaata
     8341 gctggcttcc atcggctcga tgctcagtcc ctgcggcgcg caaacctcga gggcgagcgc
     8401 cacgtccggg cgctccatga agccgtagcg cgcgacgacg cgccagcaat cgttggcgag
     8461 cggctcgcac cgtaccgcct cggcgtcggg catccacggc acgtcggcga actcaacggt
     8521 caggaacacg ttccgtgcat gcagcacctt gtagtgcttg aggctgtgca gcagcgcgtt
     8581 cggcgtcgca tcgggactcg acgtcagaaa tacggcggtg ccggggacgc gctgcggcgg
     8641 gtctgcgaac agcgccgcaa gcagtcccgc cagcggcggt gatccgccgc gcaggcgctc
     8701 gagcagcaag gcgcgtccgc gccgccaggt aagcatcacg aagaagacga cgccgccgag
     8761 cgcgagcggg aaccacccgc cgtcgcggac cttgttcatt gccgcggcga agaacgcggt
     8821 atcgacgaac atgaagaacg cggtcgccag cgtgcacagc cagcccgggt aatgccacgc
     8881 gtagcgcagc acgaagaacg tgagcagcgt cgtcacgagc atcgtgccca tgacggcaac
     8941 gccgtacgcc gaagcgaggc gcgacgagct gccgaaaccg acgacggcgg ccgccaccat
     9001 gacgagcagc gtccagttca ccgcggggac gtagatctgg ccgatggtcc gtggcgaggt
     9061 atgcacgatc gacaggcgcg gcaggtagcc gagctgcacc gcctgcttcg tcatcgagta
     9121 cgcgccggaa atcgtcgcct gcgatgcgat gaccgtggcg gcagtggcga ggacgatcat
     9181 cggatacagc gcccacgccg gatacgacag gtagaacggg ttcgcgaggg cggtgggcgt
     9241 cgcgatcagg agcgccccct ggccgaagta atccaggacc agcgcgggtg ccgccacgcc
     9301 gaaccacgcg atgcgaatcg cgcgctttcc gaaatggccc atgtcggcgt agagggcttc
     9361 cgcgccggtg acggcgagga ggaccgcacc gagcacgacg aacgatgccg atccgtgctg
     9421 cgtggtgaag cgcagcgcgt gtaccggatc gagcgccgtc aggattaccg gggccttgcc
     9481 gatattccac gcgccggcgc atgcgagcga cacgaaccag atcgcgcaca cgggtccgaa
     9541 cagccggccg acgaagcccg ttccgaatcg ctggatcgcg aacagcgcga tcaggatcac
     9601 cgtagcgatc ggcacgacgt agggtgcgag cgcgtcggtg ccgacttcga gaccctcgac
     9661 ggccgacagc accgagatcg ccggggtgag gaccgcgtcg ccgtagaaga gcgaagcgcc
     9721 gaacacgccg atcacgagca acgcggcgcg aagcctcgga tgcgcgcgca cagacgaggt
     9781 cgccagcgcg agcagcgcca tgatgccccc ctcgccgcga ttgcttgcgc gcagcacgag
     9841 cgtcacgtac ttgagcgaga cgatgatcat cagcgcccag aagatcgccg agacgccgcc
     9901 gatgacgttc tccggcgtca gcggtatgcc gtgctgcgga ttgaacgtct ccttcgcggc
     9961 atagagcgga ctcgtgccga tgtcgccgta cacgacgccg agtgcgagga gtgccagcgc
    10021 cgcgctgcct tcgcgcgttc gcgacggggc ggcgatgccg ggaactgcaa tcgcgggatg
    10081 cggggtatcc atggcgaaga gcttagccgt gcgcagcacc gattcatcgc gccgccgcgt
    10141 atttttacgg cctctttatg cggcgtgcga tagagtcggg ccgtgaagcc gacgctttgc
    10201 aacgccacgt gaagagcgca cacgagagcc ggccgcgaat cgtgaagacg caaccgcgcc
    10261 tcgccggcta cggcgcagcc gtgctggccg ccacggcggg aacgctggcc ggctgggcga
    10321 tgcgtccgcg cttcgacata gtgaacatcg cgatggtgta cctgctcgcc gtcgtcatcg
    10381 tttcgctgcg cttttcgcgc ggcgcggcca tcgtgacgtc gatcctctgc gtggcggcat
    10441 tcgattacct gttcgtcccg ccgcgcggca cgttcaccgt cgcggacatc cagtacctgc
    10501 tgacgttcgc gatcatgctg gcggtggcgc tcgtcatctc gcatcttgtc gacagcgtgc
    10561 gccggcaggc ggcgcggcag gcgtcgctca ctgtcgacgc cgagaccgag cgcatccgca
    10621 gcgcgctgct cgcgtcgatc tcgcacgatc tgcgcacgcc gctcgccgtc ctcgcgggcg
    10681 catcgtcgag cctcgcgagc agcggcgatc ggctgagcgc cgtcgagcgc gaggcgcttg
    10741 cgacaagcgt cgtgcggcag gcgcgcgaaa tgtccgagca tgtcgagaag atcctgcaga
    10801 tgacgcgact cgaagccggc gcgatccgcc tcgatcgcga ctgggcgtcg ctcggcgaaa
    10861 tcgtcgggtc ggtcctgctg cggcttggcg agcgcctcgc gggccaccgc atcatcgtcg
    10921 agatcccggg cgatctcccg ctcgtgcgtg tcgatgcgac gctgatcgag caggccctta
    10981 tgaacctgct cgacaattgc gcgcgccaca cgccggccga tacggtggtg cggctgcgtg
    11041 cgaaggcccg tgcggccgac atcgtcgtca cggtcgagga ctacagcggc ggtctttccg
    11101 atcacgattt cgagcggctt tttgcgaagt tccatcatgc ggcggccgag ggcagtggca
    11161 gtggcgtcgg cctgggcctc gccatctgcc gcgcaatcgt gcgtctgcac gacgggcagg
    11221 cgtgggccga gcgcgtcccg ggcggcggta ccgcatttcg cttcacgctg ccgcgggagc
    11281 catcgccggc agcgccggcc gagccctgag atgaccgatc cgcggcccac ggtgctgatc
    11341 gtcgaggatg agcccgagat ccgccgtttc ctgcgcgcga cgctcgccgc gaacgattat
    11401 cgcgtcgtcg aatcagccac cggacgccgc ggccagatcg acgctggcac ccacaagccc
    11461 gatctcgcca tcgtcgacct cggattgccc gacgtcgacg gcatcgatgt catccggcgc
    11521 attcgcgaat ggtcgccgat gcccgtcatc gtgctgtccg cgcgcatcca ggagcgcgcc
    11581 aagatcgacg cgctcgacgc gggcgccgac gattatgtga cgaagccgtt tgccgtgggc
    11641 gagctgctgg cgcgggtgcg ggttgcactt cgccacgcgg tgcgaggcgc agcagtgacg
    11701 tccacgctgc ggatcggcgc tgcgtgcgtc gacctcgagc ggcgaaccgc gcggcgaggc
    11761 gaggacgacg tccgccttac gccgatcgag tttcgcctgc tcgcgacgct cgcgcgccac
    11821 ctcggcctgg tcgtcacgca tcgccagttg ctgaccgagg tgtggggacc gacgcatgcg
    11881 tccgacacac attacctgcg catctacatg aagcagcttc gcgacaagct cgagcccgat
    11941 ccgctgcggc cgcggcacct gatcacggaa acgggaatcg gctatcgctt gctcggcgac
    12001 gagtagccgc ggcggcgcca cgcccggctg gtaagatgat tcagcgctcg ttcttacgtc
    12061 ggcaatgtcg actccggtta tctgtccgcg ggccgccgat ctgtattcct gcttcctgat
    12121 ctttgatccc tgatccctga tgcggccgat cgccgtcttc cgcttcagcc cgaccgaagg
    12181 gccggcgcat ttcgcgcagt ggctcgatgc ggccgcattg ccgtggcagc tgatcgccgt
    12241 cgacgagggc gacgcgattc cggccgacgc gacgcaattc gcgggcatcg gcatgatggg
    12301 cgggccgatg agcgtcaacg atgtgctcgc ctggatcgat cccctgtcgc ggctgcttcg
    12361 ccacgccatt gcgcgggaca tcccggtgat cggccattgc ctcggcggcc agctgctcgc
    12421 gcaggcgctc ggggcgcgcg tgcgccgcac gcagacgccg gaaatcggct ggatcgacgt
    12481 cgagtcgacc gacgccgcgg cccgcgatgc gtggttcggg ggccgcgcgc acttcacgcc
    12541 gttccagtgg cattacgacg tgttcgagct gccgcccggc gcatcccgcg tgctgacgaa
    12601 ctcgttcaac ccgaatcagg cctatgtgat cggcgagaag cacatcggct tccagtgcca
    12661 catcgaaatg accgccgatc tcgtggagac gtggtgccga agcggtgccg acgagatcgc
    12721 cgcgccgggc acggcggcgc gccagtcacg cgacgagatt cttcgcgatc tgcacgcgcg
    12781 gctcgatgcg ctgtcgatcg tggccgatgg ggtctacgcg cgctggtcgc aggggctgc
//
LOCUS       DASSVX010000031         7717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_267945, whole genome shotgun sequence.
ACCESSION   DASSVX010000031 DASSVX010000000
VERSION     DASSVX010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267945"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="VFO53_01525"
     CDS             complement(<1..1784)
                     /locus_tag="VFO53_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HET8874982.1"
                     /translation="MTYRPNWRASLAFVHDVCMAAIAWGGIYWLRFNLELREPFLSDM
                     WSTLAWILPLQAAIFLSLGLYRGLWRFASLSDLQRIVLAAALGSIVIPVVLVMLRLTA
                     IVPRSVLFFYPIVLIFLMAGSRFVFRLWKEHRFYSPFAALGEPVIVVGAGEAGMRLTQ
                     EMARSRQWRVVGLLDDDATKHGRLLRNVTVLGPIAELALWTRKYDVRKVIIALPSANH
                     AVRRRVAELCAAAHVEALTVPSYEDLISGRTPLTMVRTVEIDDLLGRDPVVLDNAGIA
                     EWLGNRVVMVTGAGGSIGAELCRQIARFRPARLVLFDISEAALYEIRTALVDAFPQLG
                     VVSIVGDVKHASIVEETLSREKPDVIFHAAAYKHVPLMEEMNCWQAVRNNAYGTWVLA
                     RAAVVARVEKLVLVSTDKAVNPTSVMGATKRVAEQVCQSLQDGATQFVIVRFGNVFGS
                     AGSVIPRFAEQIARGGPVTVTHPDITRYFMSLSEATQLLLQAGLQGKGGEILTLDMGE
                     PVRIVDLARDMVRLYGADPDHVPIVFTGLRPGEKLYEELLAVEEATKPTTHPKLRIAQ
                     AREADTFAVGQMVTWCESDRTADDAEVRA"
     gene            complement(1781..2821)
                     /locus_tag="VFO53_01530"
     CDS             complement(1781..2821)
                     /locus_tag="VFO53_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874983.1"
                     /translation="MARDLIAAPLLAFMLAAGGIGLLRRVAGLLPQAVPNARSLHARP
                     IPRAGGYAIWLGFLPPALLFGPAFPGGLAGWVPPLLALIVVSGWDDAKEAGVVARLSV
                     HGSSALWAAVAMVLPAAAASGIASPLMLFTVTGVALVIAWSSNLYNFMDGTDGLAGTM
                     GAIGFAAYGIAAVTGADADADAARSAPAFLALCAAILPFLVVNRPRATMFLGDVGAVP
                     LGYLAAAFGAAGVLQHWWAAWFPLLVFLPFIADATLTLARRIARRERLWEGHRGHYYQ
                     RLAQLGAGHRGTLAVYAVLMAGTALTAIACHAAAVTTGWWALGLAVVVVFMLFAAIDY
                     HWRKKTGIPSPR"
     gene            3239..4360
                     /locus_tag="VFO53_01535"
     CDS             3239..4360
                     /locus_tag="VFO53_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET8874984.1"
                     /translation="MKKKLVAVAVAGVLAAPLAAQAQTANVTLYGRLNVDLEFVNGRQ
                     PDGSNPTVSRLSSNSSRFGIRGTEALGGGLSAIFQIESSINGDTNSGNLGGRETFVGF
                     QGPWGTFKMGHFLMPLDDMHPIFGNVPTYTTSILSTADIWAQGTLSKADGGFDARLGN
                     SLRYDSPSFSGLTFSGQVALRDGSGATGANTGDHASELRHAYSWGGNAIYNNGPIQVG
                     LGLEGNQKVRGQDLNDYDITVTGGYNFGIIRAALLYERLKYDTPTGDLKRDMWGISGT
                     MPMGPGTWYAFFGRAGDGKGSAADGTRVGGLAKGGDTATNQWEISYTYPFSKRTQVYA
                     GYVKLDNECNASYTFNINAYPIGVGGKPGGFVLGMIHLF"
     gene            4523..5140
                     /gene="coq7"
                     /locus_tag="VFO53_01540"
     CDS             4523..5140
                     /gene="coq7"
                     /locus_tag="VFO53_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HET8874985.1"
                     /translation="MTSLILGFDRALRTLSGTATAGRPTPGANLPDPMLGPHERRHAA
                     GLMRVNHTGEVCAQALYSAQALVARNPEVRARFDRAAREEEDHLAWTQARLAEMNDRP
                     SLLNPLWYAGSFAIGVAAGFTGDHANLGFVVETERQVEEHLTSHIDRLPASDAKSRAI
                     VEQMRDDEARHAAMALDAGGMPLPYPVRRAMRAAADVMRAVAYRI"
     gene            complement(5163..6470)
                     /gene="cca"
                     /locus_tag="VFO53_01545"
     CDS             complement(5163..6470)
                     /gene="cca"
                     /locus_tag="VFO53_01545"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915549.1"
                     /note="catalyzes the addition and repair of the essential
                     3'-terminal CCA sequence in tRNAs without using a nucleic
                     acid template; phosphohydrolase activities include
                     hydrolysis of pyrophosphate, 5'-nucleoside tri- and
                     diphosphates, NADP, and 2'-AMP with the production of Pi,
                     metal-dependent phosphodiesterase activity for 2',3'-cAMP,
                     2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic
                     substrates with the formation of 2'-nucleotides and
                     3'-nucleotides; these phosphohydrolase activities are
                     probably involved in the repair of the tRNA 3'-CCA
                     terminus degraded by intracellular RNases; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HET8874986.1"
                     /translation="MTRPLPQPVRIYRVGGSVRDELLGRPVNDTDFVVVGATPEIMLA
                     SGFLPVGKDFPVFLHPQTRDEYALARTERKHGRGYRGFQFFATPDVTLEEDLRRRDLT
                     INAMARGPDGHLIDPFGGESDLHAGVLRHVSGAFAEDPLRVLRVARFAARFGFRIADE
                     TLALMRAIVRSGELDTLAPERVWQEVSRGLCEAHPSQMLDALRTCGALAAVLPEIDAL
                     YAISLDDGEPDGVRAHDDAGAFTARALDWAAQRAMSLPARYAVLAQELDRVGADRVDA
                     RTGIARAQALSARLRAPLECRDAARLAVRWHRAIAAAETLTPAALLDLLLAADALRRA
                     ERLDTLASAAAAHVAAHRVAKPNGRRRTGLLHEALAVVRGVDAAAIARDAIAHAAADG
                     THASAGDAIASALRDARLAALRDWKTARKRGRPRSAGAPRADE"
     gene            complement(6467..7405)
                     /locus_tag="VFO53_01550"
     CDS             complement(6467..7405)
                     /locus_tag="VFO53_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HET8874987.1"
                     /translation="MTQRVLVAGGAGFVGRRVVARLSAAGHDVIVLTRARERARDLLL
                     LPTVRVIEADPFDAAVLARWTRHATAAINLVGVLHERGRDTFERVHVDFPRTLLAACR
                     KAGVSRMLHMSALAAAADAASLYLRSKAAGEAVVAESGLAWTIFRPSVIFGRGDSFLS
                     LFARLANAFPVIPLAAASARFQPVFVGDVAECFARALVDDETAGRRYSLCGPKPYTLQ
                     ELVSYVAETSGHPRPIVALGPGLAQMQARVMEWLPGPLLTRDNLASMQHDSVCEGPFP
                     PEFGITPTPIEAIAPDYLAPVALKSRFDTFRANSGR"
     gene            complement(7402..>7717)
                     /locus_tag="VFO53_01555"
     CDS             complement(7402..>7717)
                     /locus_tag="VFO53_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET8874988.1"
                     /translation="LNTQFGAFYFKHWLDRLGGLPVLAAAAYNAGPSRAQAWRPAAAP
                     MEGAIWVETIPFDETRDYVKRVLANTMLYTRALDRPYVPLTQRLGVVTPRDLDSVAAS
                     EP"
BASE COUNT         1315 a         2669 c         2654 g         1079 t
ORIGIN      
        1 gcgcggacct ccgcgtcgtc ggcggtgcga tcgctctcgc accacgtgac catctgcccg
       61 accgcgaacg tgtcggcctc gcgcgcctgc gcgatgcgca gcttggggtg cgtcgtcggc
      121 ttggtcgcct cctcgacggc aagcagctcc tcgtagagct tctcgccggg gcgcaacccc
      181 gtgaacacga tcggcacgtg gtcggggtcc gcgccgtaca ggcggaccat gtcgcgcgcg
      241 agatcgacga tgcgcaccgg ctcgcccatg tcgagcgtca atatctcgcc gcccttgccc
      301 tgcagccccg cctgcagcag cagctgcgtc gcctcggaca gcgacatgaa gtagcgcgtg
      361 atgtccgggt gcgtgaccgt gaccggcccg ccgcgcgcga tctgctcggc gaagcgcggg
      421 atcacgctgc ccgcgctgcc gaacacgttg ccgaagcgca cgatgacgaa ctgcgtcgcc
      481 ccatcctgca ggctctggca cacctgctcg gcgacgcgct tggtcgcgcc catcaccgac
      541 gtggggttca ccgccttgtc ggtggacacg agcacgagtt tctcgaccct ggccaccacc
      601 gcggcgcggg cgagcaccca cgtgccgtag gcgttgttgc gcaccgcctg ccagcagttc
      661 atttcctcca tcagcggcac gtgcttgtac gccgccgcat gaaagatgac gtccggcttc
      721 tcgcgtgaca gcgtttcctc gacgatcgac gcgtgcttga cgtcgccgac gatcgacacg
      781 acgcccaact gcggaaacgc gtcgacgagc gcggtcctga tctcgtagag cgccgcctcc
      841 gaaatgtcga acagcacgag ccgcgcgggg cgaaagcgcg cgatctggcg gcagagctcg
      901 gcaccgatcg acccgcccgc gccggtcacc atcaccacgc ggttgccgag ccactcggcg
      961 attccggcgt tgtcgagcac gacggggtcg cggccgagca gatcgtcgat ctcgacggtg
     1021 cgcaccatcg tgagcggcgt gcggcccgag atcaggtcct cgtacgaggg caccgtcaac
     1081 gcttcgacat gcgccgcggc gcaaagctcg gcaacgcggc ggcgcacggc gtggtttgcc
     1141 gatggcagcg cgatgatcac cttgcgcacg tcgtatttgc gcgtccacag cgcgagctcg
     1201 gcgatcggcc cgagcaccgt gacattgcgc agcagccgcc catgcttggt cgcgtcgtcg
     1261 tcgagcaggc ccacgacgcg ccactggcgg ctgcgcgcca tctcctgcgt caggcgcatg
     1321 cccgcctcgc cggcgccgac gacgatcacc ggctcgccga gcgccgcgaa cggactgtag
     1381 aaccggtgct ccttccacag ccggaacacg aagcgcgagc cggccatcag gaagatgagc
     1441 acgatcgggt agaagaacag cacgctgcgc ggcacgatcg ccgtgagccg cagcatcacg
     1501 agcaccaccg ggatgacgat cgagccgagc gccgccgcaa gcacgatgcg ctgcaggtcg
     1561 gagaggctcg caaagcgcca cagcccgcga tagagcccga gcgacaggaa gatcgcggcc
     1621 tgcagcggca ggatccacgc gagcgtcgac cacatgtcgg acaggaacgg ctcgcgcagc
     1681 tcgaggttga agcgcagcca gtagatgccg ccccaggcga tggcggccat gcagacgtcg
     1741 tggacgaagg cgagcgaggc gcgccagttg ggccggtagg tcatcggggc gaggggatgc
     1801 cggtcttctt gcgccaatga taatcaatcg ccgcgaacaa catgaacacc acaacgacgg
     1861 cgagcccgag cgcccaccag cccgtcgtga ccgcagcggc atggcaggcg atcgccgtca
     1921 gcgccgtgcc ggccatcagc accgcgtaga ccgcgagcgt accccggtga cccgcgccga
     1981 gctgggcgag gcgctggtag tagtgcccgc gatgaccttc ccacaaccgc tcgcggcgcg
     2041 cgatccgccg tgcaagcgtg agcgtcgcgt cggcgatgaa cgggaggaag acgagcagcg
     2101 gaaaccatgc cgcccaccag tgctgcaaca cgccggcggc gccgaaagcc gccgcgaggt
     2161 agccgagcgg cacggcgccg acgtcgccga ggaacatcgt cgcccgcggc cggttcacga
     2221 cgagaaaggg aagtatcgct gcacagaggg cgaggaaggc cggcgccgag cgcgccgcat
     2281 cggcatccgc atctgcgccg gtcacggccg cgatcccgta tgccgcgaaa ccgatggcgc
     2341 ccatcgtacc cgcgagtccg tcggtgccgt ccatgaagtt gtacaggttc gacgaccacg
     2401 cgatgacgag cgcaacgccc gtcacggtga acagcatgag cggcgaggcg atgccggatg
     2461 cggcggcggc gggcagcacc atcgcgacgg cagcccacag cgcactcgat ccgtgcacgg
     2521 acagccgcgc gacgacgccc gcctccttgg cgtcatccca gcccgatacg acgatgagcg
     2581 ccagcaacgg cggcacccag ccggccaggc cgccggggaa cgccggcccg aaaagaagcg
     2641 ccggcggcag gaatccgagc cagatcgcat agcctcccgc gcgcggtatc ggccgcgcat
     2701 gcagcgaccg cgcgttgggg acggcctggg gcagcaaccc ggccacgcgc cgaagcagcc
     2761 cgatgccgcc tgcggcgagc atgaacgcga gcagcggcgc ggcgatcagg tctcgcgcca
     2821 tcgacaatgg caaagccgca tcggccagcc tcgttttgcg tcgggaaact ggtttggaaa
     2881 caggcaacgg cccccgcgcg caccggatgg gcccgagtaa tctttcgaaa aacggccgtc
     2941 cggcgcgcga aagcgcggga aacgtcgcgg cgcgatgcgt ttccccttgc cgatcatcat
     3001 tttagcgcag gaacgtcagc ggaccgccgc gaaactccgc gccgcggcgg cggccgcgct
     3061 tgttgcttca gcacaacagc ggagaagagc cttcgaggct tccgtagcct tgccttgcca
     3121 atgctttccc gcgtttggca caatgggtcg caacttctcg aaagagttga gaaggttcct
     3181 gatggcaggc gcaaaaggcg gtttccttgc caaatttttc cgatagagga gagactcaat
     3241 gaaaaagaag ctcgttgctg tcgccgtcgc gggcgtcctc gctgctccgc tggctgctca
     3301 agcgcagact gcgaacgtga cgctgtacgg ccggctcaac gtcgacctcg agttcgtcaa
     3361 cggtcgtcag ccggatgggt cgaacccgac ggtgagccgg ttgagctcga actcgtcgcg
     3421 gttcggcatt cgcggcaccg aggcactggg tggtggcctg tccgccatct tccagatcga
     3481 atcgagcatc aacggcgaca ccaacagcgg caacctcggc ggtcgcgaaa cgttcgtcgg
     3541 cttccaggga ccgtggggca cgttcaagat gggccacttc ctgatgcccc tcgacgacat
     3601 gcacccgatc ttcggtaacg tgcccaccta tacgacgtcg atcctgtcga cggccgacat
     3661 ctgggcgcag ggcacgctgt cgaaggccga tggcggcttc gacgcgcgtc tcggcaactc
     3721 gctgcgctac gactcgccgt cgttcagcgg cctgacgttc agcggccagg ttgcgctgcg
     3781 cgacggttcg ggtgccacgg gcgccaacac cggcgatcac gcgtccgaac tgcgtcatgc
     3841 gtacagctgg ggtggcaacg cgatctacaa caacggcccg atccaggtcg gcctcggcct
     3901 cgaaggcaat cagaaggttc gcggccagga cctgaacgac tacgacatca cggtgacggg
     3961 cggctacaac ttcggcatca tccgcgcggc gctgctgtac gagcgtctca agtacgacac
     4021 gccgacgggc gatctgaagc gcgacatgtg gggcatcagc ggaacgatgc ccatgggtcc
     4081 cggcacctgg tacgcgttct tcggccgcgc gggcgacggc aagggcagcg cggcggacgg
     4141 cacgcgtgtc ggcggcctcg ccaagggtgg ggacacagcg accaaccagt gggaaatcag
     4201 ctatacgtat ccgttctcga agcgcacgca ggtatacgcc ggctacgtca agctcgacaa
     4261 cgagtgcaac gcgtcgtaca cgttcaacat caacgcgtac ccgattggtg tcggcggcaa
     4321 gcccggcggc ttcgtgctcg gcatgattca cctgttctaa ggcagtcgca tcaaacgcaa
     4381 cgggcgcctc gcgggcgccc gttgtttttg tggatgcggc acgcgcgacg cgggagcgac
     4441 ggcgcgctcg cgcggtccaa gcctgacggt cggcgcggat gacgcccctg acgctcgctc
     4501 taccttatcc gtaacccctg tgctgacctc gctcatcctc ggcttcgatc gtgcgcttcg
     4561 cacgctgtcc ggcacggcaa cggccggacg gccgacaccg ggcgcgaacc tgcccgatcc
     4621 gatgctcggc ccgcacgagc gccggcatgc ggcaggactc atgcgcgtga accataccgg
     4681 cgaggtgtgc gcgcaggcgc tctattcggc gcaggcgctc gtcgcgcgca atcccgaggt
     4741 ccgcgcgcgc ttcgatcgcg cggcgcgcga ggaagaggac caccttgcat ggacgcaggc
     4801 gcggctcgcc gaaatgaacg accggccttc gctgctgaac ccgctctggt acgcgggctc
     4861 gttcgctatc ggcgtcgccg cggggttcac cggggatcat gccaatctcg gcttcgtcgt
     4921 cgaaacggag cgccaggtgg aggaacatct cacctcgcat atcgaccgcc tgccggcgag
     4981 cgacgcgaaa agccgcgcga tcgtcgagca gatgcgcgac gacgaggccc gccacgccgc
     5041 gatggcgctc gatgcgggcg ggatgccgct gccgtatccg gtgcgtcgcg cgatgcgcgc
     5101 cgcggccgac gtgatgcgcg cggtcgccta caggatctga cggggcgctg ccggcggcgc
     5161 aatcactcgt ccgctcgcgg cgctcccgcg ctgcgcgggc gcccgcgctt cctcgccgtc
     5221 ttccagtcgc gaagtgccgc gagccgtgcg tcacgcaatg ccgatgcgat cgcatcgccg
     5281 gcgctcgcat gggtgccgtc cgccgccgca tgcgcaatgg catcgcgggc gatcgcggcc
     5341 gcatcgacgc cgcgcacgac ggccagcgcc tcgtgcagca ggcccgtccg ccggcgccca
     5401 ttgggcttcg ccaccctgtg tgcggcgacg tgtgcggcgg cggccgatgc cagcgtgtcg
     5461 agccgctcgg cgcgacgcag tgcgtcggcg gcaagcagga ggtcgagcaa tgccgccggc
     5521 gtcagcgtct cggcggcggc gatggcgcga tgccatcgca cggcaagccg cgccgcgtcc
     5581 cggcattcga gcggcgcgcg aaggcgcgcg gaaagcgcct gcgcacgcgc gattccggtg
     5641 cgtgcatcga ctcgatcggc gccgacacga tcgagctctt gcgccagcac cgcgtaacgc
     5701 gcaggcagcg acatcgcgcg ttgcgctgcc caatcgagcg cacgcgccgt gaatgcgccg
     5761 gcatcgtcgt gcgcgcggac gccgtcgggc tcgccgtcgt cgagcgatat cgcgtagagc
     5821 gcgtcgatct ccgggagcac cgcggcaagg gcgccgcagg tgcgcagcgc gtcgagcatt
     5881 tgcgacggat gcgcttcgca cagtccccgc gaaacttcct gccagacgcg ctcgggcgcg
     5941 agcgtgtcga gctcaccgct gcgcacgatt gcgcgcatca gcgcaagcgt ctcgtcggcg
     6001 atgcgaaagc cgaagcgtgc cgcgaagcgc gcgacgcgaa gcacgcgcag cgggtcctcg
     6061 gcgaacgcgc ccgacacatg gcgcagcacg cccgcgtgca gatcggactc gccgccgaac
     6121 gggtcgatca ggtgtccgtc gggtcccctc gccatcgcgt tgatcgtgag atcgcgccgg
     6181 cgcaggtcct cctcgagcgt gacgtcgggc gtcgcgaaga actggaaccc gcgatagccg
     6241 cgcccgtgct tgcgctcggt gcgcgcgagc gcgtattcgt cgcgggtctg cggatgcagg
     6301 aacaccggga aatccttgcc gacgggaagg aatccggacg cgagcatgat ctcgggagtc
     6361 gcgccgacga cgacgaagtc ggtgtcgttc accggacgtc cgagcagctc gtcgcgaacc
     6421 gagccgccga cgcgatagat gcgcaccggc tgcggcaacg gccgcgtcat cgaccgctgt
     6481 tcgcgcggaa cgtatcgaag cggctcttga gcgcgacggg cgcgaggtag tcgggtgcga
     6541 tcgcctcgat cggcgtcgga gtgatgccga attcgggcgg aaacggccct tcgcacacgc
     6601 tgtcgtgctg catcgacgca agattgtcgc gcgtgagcag cgggcccggc agccactcca
     6661 tcacgcgcgc ctgcatctgc gcgaggccgg gtccgagcgc cacgatcggg cgtgggtgac
     6721 ccgacgtttc cgcgacgtag ctcacgagct cctgcagcgt atagggcttc ggcccgcaca
     6781 gcgaatagcg cctgcccgcc gtttcatcgt cgacgagcgc acgcgcgaag cattcggcga
     6841 cgtcgccgac gaacaccggc tggaagcgcg cgctggcggc tgcgagcgga atgacgggaa
     6901 acgcgttggc aagccgcgca aaaaggctca ggaacgagtc accgcgaccg aagatgaccg
     6961 aaggacggaa gatcgtccac gcgagacccg attcggcaac gaccgcttcg cccgcggcct
     7021 tcgagcgcag gtagaggctg gccgcgtcgg ccgccgcagc gagcgcgctc atgtgcagca
     7081 tgcgcgagac gccggccttg cgacaggcgg cgagcagtgt gcgcgggaag tcgacgtgca
     7141 cgcgctcgaa tgtgtcgcgg ccgcgctcgt gcagcacgcc gacgaggttg attgccgcgg
     7201 tcgcgtggcg tgtccagcgc gcgaggaccg ccgcgtcgaa cgggtcggcc tcgatcacgc
     7261 gcaccgtggg aagcaggagc aggtcgcggg cgcgctcgcg tgcccgcgtc agcacgatga
     7321 cgtcgtggcc cgcagccgag agcctcgcga ccacacgcct gccgacgaag cccgcgccgc
     7381 cggcgacgag aacgcgctgc gtcatggctc cgaggccgcg acgctgtcga gatcgcgcgg
     7441 cgtgacgacg ccgagccgct gcgtcagcgg gacgtacgga cgatcgagtg cgcgcgtata
     7501 gagcatcgtg ttcgcaagga cgcgcttcac gtaatcgcgc gtctcgtcga acgggatcgt
     7561 ctccacccag atcgcgcctt ccatcggggc cgcggcgggc cgccaggcct gcgcgcgcga
     7621 cggcccggca ttgtaggccg cggcagccag cacgggcagc ccgccgagcc gatcgagcca
     7681 gtgcttgaag tagaacgcgc cgaactgcgt gttgagc
//
LOCUS       DASSVX010000032         3800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_51264, whole genome shotgun sequence.
ACCESSION   DASSVX010000032 DASSVX010000000
VERSION     DASSVX010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51264"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1526)
                     /locus_tag="VFO53_01560"
     CDS             complement(27..1526)
                     /locus_tag="VFO53_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET8874989.1"
                     /translation="MLVLASGADLESANPLVTTHPLSRQIQRYALFVTLLRYDSTLTP
                     QPYFARSWHWSDVGRTLTLSLAPDLRWHDGVPTTSRDAAFTFLAARDPATGFPRAAEL
                     TSLDTAVAVDDTTLQLHFSRAPSRLPPLLAELPIIPEHLLSTIPRSAMRGAPFNGAPV
                     GNGPFRFVGRRRGASWTFARNDAFPSSLGGPPALRGLVIAVVDEATTKFAGLASGELD
                     MAGIAPTMAALAKRDATLRVLTYPIPFSVGLFFNTTRPPFDDARVRLAIARSIDRPRI
                     VEIALAGFGTPASTPVPPESPLAWREAPPQIDTLRADSLLNSAGWRRGTDGVRVRNGQ
                     RLQAELLTVGTGDNVIEQLLQADLAARGIELRIRQTEMGTFLTTARASDKRFDMLLAG
                     ITGDLSLSYIDALFSGRQRGGALDYTGYHTAALDALLDAARDAAPGEVEHRAWRDVQA
                     ALDTLAPATWIYHARGLQGVSRRVHGDRMDLRGELVTVHDWTLATSGTR"
     gene            1608..2612
                     /locus_tag="VFO53_01565"
     CDS             1608..2612
                     /locus_tag="VFO53_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8874990.1"
                     /translation="MISPVASRSLGVAGALLRTAVLYWCVLSLTFVLLRMAPGDPATF
                     LLPPNASATDAVRLRANLGLDQPIAAQYARWTGRMLRGDFGTSFSEGRPVVAVLRDAL
                     PVSLALGGASLLLSFLVGTIVGVVQGARRGTHADFTLTFISVVLAASPAYWLGLGAIA
                     LFTYVASLLSFPLWARLPAVGMTTPGAELHGLAHLADLLRHSILPVTLLAAIGAGGVA
                     RYVRTSALEVLDTDWVRTARAKGLPERTVLGRHLLANMRAPLITLFALALPGTVAGSV
                     FVETIFGWPGMGRLMVISITARDYPVVLGSAAAYALLVIVANLVAESLLPWADPRLRE
                     "
     gene            2617..3486
                     /locus_tag="VFO53_01570"
     CDS             2617..3486
                     /locus_tag="VFO53_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8874991.1"
                     /translation="MRELRPRLRAVSAGSVGLAALVLLALAVIVVPALASRDPSQIND
                     VLARRLVPPLGRDAHGTMHLLGTDRFGRDLLVRMMLAGRLSLLVGVIGAGVSGLVGTL
                     VGALAGWRRGVLDRALMALADLLLALPRIVLLLVGVALWAPGLTTILVVLIATGWMGV
                     ARLVRAEVIAVSARPFVAAASALGAAPHRVLFRHVLPNAIGPAFVATTLGVGNAILLE
                     SGLSFLGLGIQPPAPSWGNMIAGGRDLLVTAPWVALAPGIALVVTVLATSVVGDEWRD
                     RSRGAMRSPSSRA"
     gene            complement(3473..>3800)
                     /locus_tag="VFO53_01575"
     CDS             complement(3473..>3800)
                     /locus_tag="VFO53_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874992.1"
                     /translation="DDVLLPIKGETLRANLLDPREVGLIELSGDGLVFSSAKPAQREG
                     WIALRCVNRREVAVRGTWTFGRTIVESVRARLDETPEGPVSVNGRSVTFDAAAKEILT
                     VLVKLD"
BASE COUNT          562 a         1217 c         1312 g          709 t
ORIGIN      
        1 aagatcttcc gctgagatga gaatgctcat cgggtgccgg atgtggcgag ggtccaatcg
       61 tgaaccgtca cgagctcgcc gcggaggtcc atcctgtcgc catgcacgcg acgcgagacg
      121 ccctggagtc cgcgtgcgtg atagatccac gtcgctggcg cgagggtatc gagcgccgct
      181 tgcacgtcgc gccatgcacg gtgttccact tcaccgggcg cagcgtcccg cgcggcgtcg
      241 agcagagcgt ccagtgccgc ggtgtggtaa ccagtgtagt cgagcgcgcc gccgcgctgt
      301 cgccctgaga agagcgcgtc gatgtacgac agtgagagat cgccggtgat tccggcaagc
      361 agcatatcga agcgtttgtc agacgcgcgc gcggtggtga gaaaggtgcc catctccgtc
      421 tgccgtatcc gcagctcgat cccgcgagcg gcgagatccg cctgcagcag ctgctcgatt
      481 acgttatcgc cggtgccgac tgtgagtaac tcagcctgca atcgctgtcc gttgcgaacg
      541 cgcactccat ctgtgccgcg gcgccagccc gccgagttca gcagcgagtc ggcgcgcagc
      601 gtgtcgatct gcggcggcgc ctcgcgccag gcgagcgggc tttcgggtgg gacaggcgtt
      661 gatgcgggag tgccgaagcc ggcgagcgcg atctcgacga tgcgagggcg atcgatcgat
      721 cttgcgatcg cgagtcgcac gcgcgcgtcg tcgaaaggcg ggcgcgtggt gttgaagaaa
      781 agtccgacac tgaacggtat cggatacgtg agcacgcgga gcgttgcgtc gcgcttcgcg
      841 agcgctgcca tcgtcggcgc gatccctgcc atgtcgagct caccactcgc cagcccggcg
      901 aatttcgttg tcgcctcgtc gacaaccgcg atcaccagcc cgcgcagcgc tggcggcccg
      961 ccgagtgagg agggaaacgc gtcgtttcgc gcgaaggtcc agctcgcgcc gcgccgccgt
     1021 cccacgaagc gaaagggacc gttcccgact ggcgccccgt tgaacggcgc gccacgcatc
     1081 gccgagcggg gaattgtgga aagaagatgc tcggggatta tcggcagctc ggcgaggagc
     1141 ggtgggagtc gagagggcgc gcgactgaag tgcagctgca aggtcgtgtc gtccacggcg
     1201 accgcggtgt cgagtgaggt gagctcagcg gcgcgcggaa atcccgtggc cggatcgcgc
     1261 gccgcgagaa atgtgaaagc tgcatcgcgt gaagtagtgg ggacgccgtc gtgccagcga
     1321 agatcgggag cgagcgagag cgtcagcgtg cggccgacat ccgaccagtg ccagcttcgc
     1381 gcgaagtatg gttgtggagt gagcgtcgag tcgtaccgca ggagcgttac gaacagcgcg
     1441 tatctctgga tttggcgaga gagcggatgc gtcgtcacca gcgggttcgc tgattcgaga
     1501 tccgcacccg acgcgaggac gagcacatcc ggctgccgtt gcggtgtgca cgaggtgagc
     1561 acgagtgcag caattgacgc gccgagtgtc cgaggcgata tcatcgcatg atttcgccag
     1621 tcgcatcgcg ctcgctcggc gtcgccggcg cactccttcg cacagcagtg ctgtactggt
     1681 gcgtgctgtc gctcacgttc gtgctccttc gcatggcgcc gggagatccg gcgactttcc
     1741 tgctgccgcc caacgcatcg gccactgatg cggttcgact tcgcgccaac ctggggctcg
     1801 atcagccgat cgccgcgcaa tatgcacgat ggacggggcg gatgctgcgc ggcgatttcg
     1861 gaacaagctt cagcgaggga cgacctgtcg tcgcagtgct ccgtgacgcg ctccccgtct
     1921 cgctcgcgct cggcggcgcg tcgctgctac tctcctttct cgtcggcacg atagtgggcg
     1981 tggtgcaggg tgcgcgccgc ggaacgcacg ccgatttcac actcaccttt atttccgtcg
     2041 tcctcgccgc gtcacccgcc tactggctcg gtctcggagc gatcgctctg ttcacctatg
     2101 tcgcatcgct gctcagcttt ccgctgtggg cacggcttcc ggcagtaggg atgaccactc
     2161 ctggcgccga gctccacggg cttgcgcatt tggctgatct gctacgccac tcgatcctgc
     2221 cagtgacatt gctcgcggcg atcggcgccg gcggtgtcgc gcgttacgtg cgcacgagcg
     2281 cgctcgaagt gctcgacacg gactgggttc gcactgcgcg cgcgaagggg cttccagagc
     2341 ggacggtgct tggccgtcat ctgctcgcca acatgcgcgc cccgttgatc accttgttcg
     2401 cgctggctct tcccggaacc gtcgcgggct cggtgtttgt tgaaaccatc ttcggatggc
     2461 cggggatggg acgattgatg gtgatatcaa ttacggcgcg cgactatccg gtggtactgg
     2521 gtagcgccgc tgcctatgcg ctgctggtga ttgtcgcgaa cctcgtggcg gagtcgctgc
     2581 tgccatgggc cgacccgcgc ctgcgtgaat gagacgatga gggaattgcg tccgcggctg
     2641 cgcgccgtga gcgcgggcag cgtcggcctg gcggcgctcg tgcttctcgc cctcgccgtg
     2701 atcgtcgtgc cggcgctcgc ttcgcgtgac ccgtcgcaaa tcaacgacgt gctggcgcgc
     2761 cggcttgttc cgccgctggg ccgcgacgcg cacggcacca tgcacctgct tggcaccgac
     2821 cggttcggcc gcgatctcct cgtgcgtatg atgctcgccg gacgactgtc gctgcttgtc
     2881 ggtgtcatcg gtgcgggggt ctccggtctc gtgggcacgc tagtcggcgc gctcgccggc
     2941 tggcgccgcg gcgtgctgga tcgcgcgttg atggcgctcg ccgatctcct gctcgcgctg
     3001 ccccgtatcg tgctactcct ggtcggcgtt gctctctggg cacccgggct cacgacgata
     3061 ctcgtggtac tgatcgcaac cggctggatg ggcgtcgcgc ggctggtgcg cgccgaggtg
     3121 atcgcggtga gcgcgaggcc attcgtcgcg gctgcgtcgg cattgggggc tgcaccgcac
     3181 cgcgtgctgt tcaggcacgt gctcccgaac gcgatcgggc ccgcatttgt tgccacgacg
     3241 ctcggcgtcg gaaacgcgat tcttctcgag agcgggctgt cgttcctggg gctcgggatc
     3301 cagccaccgg cgccgagttg ggggaacatg atcgcgggcg gtcgcgacct gctggtgacg
     3361 gcgccgtggg ttgcgctggc gccgggcatc gcgctggttg taacggttct ggcgacgagc
     3421 gtggttgggg atgagtggcg cgatcgaagt cgcggtgcga tgcgctcgcc atctagtcga
     3481 gcttaacaag caccgtcagt atctctttgg cggcggcatc gaacgtcact gatctcccat
     3541 tgacgctaac cggcccttct ggtgtctcgt ccagccgcgc gcgcaccgac tcgacgattg
     3601 ttcgtccgaa cgtccaggtc ccccgcacgg cgacctcgcg ccgatttacg cagcgcaacg
     3661 caatccaccc ctctcgctgc gccggcttcg cggagctgaa gacgagtcca tcacccgaca
     3721 actcgatcaa gcccacttcg cgcggatcga gcaagttcgc gcgcagggtc tcgcccttga
     3781 tcgggagcag gacgtcgtca
//
LOCUS       DASSVX010000033        16887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1284346, whole genome shotgun sequence.
ACCESSION   DASSVX010000033 DASSVX010000000
VERSION     DASSVX010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 16887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16887
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284346"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /gene="fdx"
                     /locus_tag="VFO53_01580"
     CDS             <1..271
                     /gene="fdx"
                     /locus_tag="VFO53_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HET8874993.1"
                     /translation="GVSVLDNLLEQGIEVEHACEKSCACTTCHVIVREGFDSLREASE
                     DEEDLLDRAWGLTPVSRLSCQAMVDDARLVVEIPRYTINYAKERG"
     gene            276..470
                     /gene="iscX"
                     /locus_tag="VFO53_01585"
     CDS             276..470
                     /gene="iscX"
                     /locus_tag="VFO53_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HET8874994.1"
                     /translation="MKWTDTLDIAMALRAAHPGVVPSVVRFTDLHNWVQKLPDFDDDP
                     KRGGEKILEAIQQAWIDEID"
     gene            complement(752..1603)
                     /gene="serB"
                     /locus_tag="VFO53_01590"
     CDS             complement(752..1603)
                     /gene="serB"
                     /locus_tag="VFO53_01590"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HET8874995.1"
                     /translation="MILHHDDLVIQGPQVLREDIEILRLMTGASGVEPLARASCEAFM
                     LHEPEHRAGVAEACAAAGYDFAFVPCERALGRVRLVAMDMDSTLITIECIDEIAALHG
                     IGDEVAAITASAMRGEIDFRASLTRRVALLEGMPVDALARVHDERLQLSPGVPRLFDA
                     LRAIGAKTLLVSGGFTFFTERLQSRLGIDYTLANVLDVEDGRLTGRMRGDIVDADAKA
                     VRFAELAAEYAAGGIGVAIGDGANDLAMLARADVSVAYRAKPRVRAQAMHAIDHCGLD
                     AVLNLFA"
     gene            complement(1600..4098)
                     /gene="rnr"
                     /locus_tag="VFO53_01595"
     CDS             complement(1600..4098)
                     /gene="rnr"
                     /locus_tag="VFO53_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET8874996.1"
                     /translation="MSKTIPLRRRAATTPRSSERPRTPEAAAHGESKPTCDAILRELT
                     EAGIPLAPAEIASRLSIGRRQQHLFDACVATLERDGEILVNRKGELCIVAKLDLIRGT
                     VQGHPDGYGFLVPEGGGDDLFLSPREMHKVLHGDRVTAKRIGVDRRGRPEGEIVDVLE
                     RANREIVGRIHEEHGIWFVEAENRRINQDLLLGAGDRGGAQPAQVVVVEIVEQPSAHG
                     EAVVRVKEVLGSATDPGIEIEIALRKHDLPFEWNPAAKRQVLRLPAEVRPADRKSRTD
                     LRHLPLVTIDGETAKDFDDAVSCEPDGRGFRLIVAIADVAHYVRDGDALDREARERGT
                     SVYFPRRVIPMLPEALSNELCSLKPDVERLCMACDMRIDARGTIKSYAFYPAVMHSRA
                     RLTYTQVYTWLNDTAAAKGTAGEVLLPHLAQLYTLYKVLLAARERRGAIDFDTAELAL
                     QFDPYGKIAEIVPAPRNDAHRLIEECMLAANVCAAGFLAENGQPALYRVHEGPPPEKL
                     AVLREFLASCALSLTGGDKPTAMDYAALIDRIRERPDFALLQTVLLRSLSRAEYSPDN
                     VGHFGLAYDAYAHFTSPIRRYPDLLVHRAIKAVLAGTPSRRGAAPRRESAPSGGSEAA
                     RAASVGEPSRRGAAPRRESAPSGGSEAAPAASVGGPYYTPKDATWAELGVHCSMTERR
                     ADDASRDVLQWLKCHYMQEKVGETFPGTISGVASFGIFVTLDGLDIDGLVHVTELPRD
                     YFHFDAVRHALIGERSGRTFQLAGRVMVKVARVDLERAKIDFTLDEAATRPSESLAPG
                     RPVSLQPSQPAAAKPIVREALARSERPRGRKSRR"
     gene            4139..4225
                     /locus_tag="VFO53_01600"
     tRNA            4139..4225
                     /locus_tag="VFO53_01600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4173..4175,aa:Leu,seq:cag)
     gene            4398..6050
                     /locus_tag="VFO53_01605"
     CDS             4398..6050
                     /locus_tag="VFO53_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8874997.1"
                     /translation="MAAGEVTVDPRVRRGLPLFVVPVLCAAAMYFRFFAGYWLGDDVG
                     NLDRAWLADQSGELWHQAWTQLSSGASSQGAFYRPLMLASILVNGWLAGSVYVGWFAV
                     SIAIHLANVAFIGMLVSRLAHACGRNGRVAGAVAALFFALCPLLAEGVFWISARADAF
                     VTLLTLSALFAWSVSRTSVGQALWPPLLLAVALGFKESAAVFALQMLLVALAWPVRTT
                     RAQRIAIAAGFALVAMFLVLRGHLFGDAWRVYYAGAGTPSLDRLREAIVSLPPWWSAL
                     THSTPHVANVYAALAALASVALLFAVTSHSSAARLVAALLASSAGLVAATFLNLGSMG
                     DTGEGGRLAYTPIAWLALALGVAGAAPVGAVERRARGEAARLPGIGLLLLACVAGSWV
                     LEGELAGASRAERGMRALAASIPDWARSNPGLTLLVIPEHDGPVITGRNAQAGLVMPP
                     LQREALLHRVLPTVPREVQARYDQLAAGLASRLATLRPSRVDAPMLARIFAPDTARWP
                     DRYACWSPGKRRIVELSAPNPEDRARWVDELEQAAEQCPPRR"
     gene            complement(6114..6635)
                     /locus_tag="VFO53_01610"
     CDS             complement(6114..6635)
                     /locus_tag="VFO53_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET8874998.1"
                     /translation="MTLDIDWPRYNTLVERLALNVHESGFRFNQIICIARGGLRVGDV
                     LSRIFDQPLAILSTHSYTAEGGTIRGELVIAEHMTMTKPRLGDRVLLVDDMVDSGHTL
                     AAVHDELPRRFPHISELHTAVLWWKACSVFEPDYYVEHLPDSPWIRQPFEVYDDLDPA
                     SLKDRLGVALERD"
     gene            complement(6632..7951)
                     /locus_tag="VFO53_01615"
     CDS             complement(6632..7951)
                     /locus_tag="VFO53_01615"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET8874999.1"
                     /translation="MTTKVVSDPRNVVVIGAQWGDEGKGKIVDWLTESAQGVVRFQGG
                     HNAGHTLVIGGRKTVLRLIPSGVLREGVAILIGNGVVLSPSALMQEIEELEQAGVDVR
                     SRLMISPACPLVLPIHVALDQAREAALGDALKIGTTGRGIGPAYEDKVGRRALRVQDL
                     LDPARFAAKLEQLLDLHNFTLVHYFKCAPVDFAKTKDDVLALGGAIAPMVGDVAAAIQ
                     RARARGESLLFEGAQGALLDVDHGTYPYVTSSNCVAGAAAPGAGIGPHMIDYVLGIVK
                     AYTTRVGTGPFPTELTDDTGAMLAKRGNEFGSVTGRPRRCGWLDIPALRRSFALNGVN
                     GQCITKLDVLDGMAQIRICTHYEVGDATVDLIPTGADEVARCRPVYETLPGWSGSTVG
                     AKSFAALPAAARAYLARIEALTGVPIAMVSTGPDRDETILVHHPFAR"
     gene            complement(7991..8971)
                     /locus_tag="VFO53_01620"
     CDS             complement(7991..8971)
                     /locus_tag="VFO53_01620"
                     /inference="COORDINATES: protein motif:HMM:NF008935.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET8875000.1"
                     /translation="MRNWLLPEAIDDVLPAEAAGLEALRRTLLDHFAHHGYRLVRPPL
                     IEHLDSLLTGSGRDLELLTFKTVDPQSGRLVGIRADITPQVARIDAHLLNEPGATRLC
                     YAGSVVRAVAEGPGATREVVQVGAELFGVPGTDGDREVIGLLVSSLTAAGVRGLHLDL
                     GHMGVYRALASAAGLAESDDSALFEALRTKDGPGVHALARHLPPAWRDALEALPTLYG
                     PAPEVLRTARERLPDIASIANALASLEALVETAAPLVDALHIDLADLRGYHYHNGAIF
                     SVFTAGEPNAVGKGGRYDGIGKAFGRARPATGFTLDLRQLAGVLARGEPK"
     gene            complement(8980..9162)
                     /locus_tag="VFO53_01625"
     CDS             complement(8980..9162)
                     /locus_tag="VFO53_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HET8875001.1"
                     /translation="MPDSLWTALALLLILEGLLPFAAPRVWREGFRHLTELTDGQLRF
                     IGMIAIGIGLLALYLI"
     gene            complement(9181..10065)
                     /gene="hflC"
                     /locus_tag="VFO53_01630"
     CDS             complement(9181..10065)
                     /gene="hflC"
                     /locus_tag="VFO53_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HET8875002.1"
                     /translation="MPLLAALVAVLFIASQSVYTVDQRKYAIKFQLGEIVETKTTAGL
                     YFKVPLVQNVKFYDKVNLTLDNPEPDRVTTSEKKPLLVDFVVLWRITDVGQYYRSVQG
                     DEEAARRRLSQTVRANLAEEFNKHDVHEAISSARDKITSVTQQKANADAKSIGVEVID
                     VRLRRVELPPDVTGPVYQRMESERRRVANELRSLGAAESEKIRADADRQRTVILADAY
                     SEAQKIKGSGDAKASAVYAQAYTQNPEFYSFYRSLEAYRATFRSKSDLMVLDPSSDFF
                     QYFKDPGTSRTAPRTPAK"
     gene            complement(10133..11323)
                     /gene="hflK"
                     /locus_tag="VFO53_01635"
     CDS             complement(10133..11323)
                     /gene="hflK"
                     /locus_tag="VFO53_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HET8875003.1"
                     /translation="MSLNDPQWGKRSNNSGPPDLDEIWRNVNRRMNELFGRRRESPDG
                     GDGGGGGMHLPLGGAGMLIVLVVVIWLASGFYIVDEGRRGVVTRFGKYTETTQPGPRW
                     HLPFPIETVELVDFSQVKTIEVGYRNTPKNKIDKEAVMLTDDENIIDIQFAVQYNLKS
                     AEDYVFNNRRPDQIVAYVAESAIREVVGKAKMDFALYEGREQIAKQTEQLMQQNLDRF
                     GTGVFVQKVTLQNVQPPDKVQAAFDDAVKAGQDRERLKNEGQAYANDVVPRARGMAAR
                     LLEEANGYKSEVEQRAQGDAQRFNQVLVEYQKAPGVTRERLYLDAMQSVLGNSSKVLV
                     DQKSGSLLYLPLDKLIQQSASPAPGAAVPDATATPPRAMPSDNALSIDASRSRDALRN
                     REGR"
     gene            complement(11389..12528)
                     /gene="hflX"
                     /locus_tag="VFO53_01640"
     CDS             complement(11389..12528)
                     /gene="hflX"
                     /locus_tag="VFO53_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET8875004.1"
                     /translation="MLERPEIGDRALVVALDIGRVDGSDRLAEIAALAASAGATVVGN
                     LRGRRPRPDAALFAGRGKVDEIAAQRREADADLVIFDHTLSGVQQRNLEQALECRVVD
                     RTSLILDIFAQRARSAEGKLQVELAQLNHMATRLVGGWTHLERQKGGIGLRGPGETQL
                     ETDRRLITQRVKLLRERLRRVARSRATQNRARRRAAVRTVALVGYTNAGKSTLFNRLA
                     AAKVYVANQLFATLDTTLRRVHVQGAENIVLSDTVGFIRDLPHDLVAAFRATLAEAAD
                     ADLLLHVIDASAANRDEQIEAVDALIEEIGAGDVPQIRVYNKSDIAGTVPGVERAPCG
                     TISSVRVSAVTGAGIAELRAALAERFPASPLPVSEPTDTPARSGL"
     gene            complement(12601..12846)
                     /gene="hfq"
                     /locus_tag="VFO53_01645"
     CDS             complement(12601..12846)
                     /gene="hfq"
                     /locus_tag="VFO53_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HET8875005.1"
                     /translation="MSNKGQMLQDPFLNTLRKEHVQVSIYLVNGIKLQGQIESFDQYV
                     VLLKNTVTQMVYKHAISTVVPSRPVAMPHQNHHEAES"
     gene            complement(12874..14208)
                     /gene="der"
                     /locus_tag="VFO53_01650"
     CDS             complement(12874..14208)
                     /gene="der"
                     /locus_tag="VFO53_01650"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET8875006.1"
                     /translation="MLPTIALVGRPNVGKSTLFNRLTKTRNALVADFPGLTRDRHYGR
                     ARAGDREFLVVDTGGFEPVATSGILFEMAKQARAAIAESDVIVFIVDAREGVTPQDHA
                     IASLLRKSGRPVVLAVNKAEGLAPERSVEFHELGVGEPLPISAAHGENVRDLIDIALE
                     HCPPPAEAEPLDEGERRIRVAVVGRPNVGKSTLVNALLGEERVIAFDQPGTTRDSIHL
                     DFERADRRYTLIDTAGVRRRGKVSEVVEKFSVIKTLQAIEDANVVILLLDAGDGITEQ
                     DAHLAGHILEAGRAMTIGINKWDAADNEQRATVKREFERKLAFLGFAEHHYISAREGR
                     GVEALLRAVDLAYAAAMARLPTPRLTRTLQLAVLRQQPPRAGYVRPKLRYAHQGGVNP
                     PLVIVHGSALSHVPDGYRRYLEHFFRDAFQLRGTPLRIQFKTGANPYANSSR"
     gene            complement(14291..15502)
                     /gene="bamB"
                     /locus_tag="VFO53_01655"
     CDS             complement(14291..15502)
                     /gene="bamB"
                     /locus_tag="VFO53_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HET8875007.1"
                     /translation="MTRAASAMPVLRALSAALVAVMLCAGCASLSESSLTSWMSSIPV
                     PSFAWLTGGSHKPGPLPTLNASVTPRIDWQQSVGRAAPGIAPAITASAIYAAAGDGTL
                     MRLDPATGRVEWKISAGQALSAGPGADASLVVVGTSKGDVLAFDTAGKARWNVRVSSE
                     VIAPPRVAEDVVVVFSGDGRIYGLSVSDGKTLWVDPRTNPPLTVRNAAGGVLSRGGLF
                     VGTAGGRLLAIDARTGTVAWDGTVATPKGATELERIADVTSLPYVDEKEACAAAYQGR
                     VACFDVVRGALIWTRDVSSLAGITADANRVYVVDDKGSVLAFDRSNGASVWKQDVLGK
                     RRIGGPQLIGDEIGVVDVEGYLHLLSRSDGAYVGRLATDGSLATTQPAPLGGGMLWQS
                     EKGTLFSVSAR"
     gene            complement(15499..16239)
                     /locus_tag="VFO53_01660"
     CDS             complement(15499..16239)
                     /locus_tag="VFO53_01660"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8875008.1"
                     /translation="MALYDLEEQDQIDDLKAWWNRYGGTVTVALVLGLLVVAGIQGWR
                     WYAGHRAENASVLYSAVSEAARTKDAAKAKDAIAQITDRYASTGYAPRAELLYAKMLY
                     DAGDRNAAKAQLAWVVDHASEEELQTIARYRLAQIQIDEKQYDTALATLDAKHPAPFD
                     GMFADLRGDALAAAGRGADARAAYESALAKLDPKSAYRNYVQVKHDAIPATAKLAESN
                     APAATAPSKAAPATAGDAVQGKAGGAVK"
     gene            complement(16226..>16887)
                     /locus_tag="VFO53_01665"
     CDS             complement(16226..>16887)
                     /locus_tag="VFO53_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET8875009.1"
                     /translation="KHPPLQDVIAAAPRLVDQLGDASRAHFDALRALLDDAGIAYTVD
                     ARLVRGLDYYNRTVFEWVTGALGAQGTVAGGGRYDGLFAELGGKPTPACGFAIGVERM
                     ILLLEAQGKAHAPALDAYVVHAGDKAGEVARHVAETLRDHGASIVLHAGGGGFKSQMK
                     KADASGARLAIIVGDDEAAAGTVAIKQLREGGDQVAVPLADVVARFAALNTGKDEYGS
                     L"
BASE COUNT         2527 a         5661 c         5809 g         2890 t
ORIGIN      
        1 cggcgtctcg gtgctcgaca accttctcga gcagggcatc gaagtcgaac acgcgtgcga
       61 gaaatcgtgt gcgtgcacga cgtgccacgt gatcgtgcgc gagggcttcg actcgctgcg
      121 ggaggcatcg gaagacgagg aagacctgct cgatcgcgcc tggggcctga cgccggtgtc
      181 gcggctctcc tgccaggcga tggtggacga cgcgcggctg gtcgtcgaga taccgaggta
      241 tacgatcaac tacgcgaagg agaggggctg actcaatgaa gtggaccgat acgctcgaca
      301 tcgcaatggc tctgcgtgcc gcgcatcccg gcgtcgttcc ttccgtcgtc cgtttcaccg
      361 acctgcacaa ctgggtgcag aagctgccgg atttcgacga tgatcccaag cgcggaggtg
      421 aaaagatcct cgaggcgatt cagcaagcct ggatcgacga gatcgactga acgttcgtgt
      481 cttcgtcgtc gtcgtttcgc gagacgcatg gctcagtcac gagcgccgga aggatgaatc
      541 ggcagctcgc cctttgtcct gcgcggggtt gtaaccgatc gcgcgtcctg ccgacaagca
      601 agccgcgcac catctttgca atcccttgtt tcatcgcttg aaaactgtcg tattcagcat
      661 gtcgcgcccg cgcgccgtaa attgttccgt cgcgcgccaa agcagcgtgc atgcgatggc
      721 tcgcactcct gcgctcgttc cacggggggc gtcatgcaaa caggttgagc accgcgtcga
      781 gaccgcagtg gtcgatcgcg tgcatcgcct gggcgcgcac gcgcggcttc gcgcggtacg
      841 cgaccgaaac gtcggcccgt gcaagcatcg ccaggtcgtt ggcgccgtcg ccgatcgcga
      901 cgccgatgcc gccggccgca tattcggcag caagctctgc gaaacgtacg gctttcgcgt
      961 cggcgtcgac gatgtcgccg cgcattcttc cggtgagccg cccgtcttcg acgtcgagaa
     1021 cgttcgccag cgtgtagtcg atcccgagcc gcgactggag gcgctcggtg aagaacgtga
     1081 agccgcccga cacgagcagc gtcttcgcgc cgatggcacg caacgcatcg aacagccgcg
     1141 gcacgccggg ggacagttgc aaacgctcgt catgaacccg cgcgagtgcg tcgacgggca
     1201 tcccttcgag caacgcgacc cggcgcgtta ggctcgcccg aaagtcgatc tcgccgcgca
     1261 tcgccgacgc ggtgatcgcg gcgacctcgt cgccaatgcc gtgcaacgcc gcaatttcgt
     1321 cgatgcattc gatcgtgatc agcgtcgagt ccatgtccat cgcgacgagc cgcacgcgcc
     1381 cgagcgcgcg ctcgcacggc acgaacgcga agtcgtatcc ggcggcagcg caggcctcgg
     1441 cgaccccggc gcgatgctcc ggttcgtgca gcatgaacgc ttcgcaagac gcgcgtgcga
     1501 gcggctcgac gccgctggca ccggtcatca gccgcaggat ttcgatgtcc tcgcgcagga
     1561 cctgcggccc ctggatgacg aggtcgtcgt ggtgcaagat cagcgacgtg acttgcgtcc
     1621 gcgcggccgt tcgcttcgcg cgagcgcttc acgcacgatt ggcttcgcgg ccgcgggctg
     1681 cgacggctgc agcgacaccg gccggccggg cgcgagcgat tcgctcggcc gcgtcgccgc
     1741 ttcgtcgagc gtgaagtcga tcttcgcccg ctcgagatcg acgcgcgcga ccttcaccat
     1801 cacgcggccg gcgagctgga acgtgcgccc gctgcgctcg ccgatgagcg catggcgcac
     1861 cgcgtcgaag tggaagtagt cgcgcggcag ctcggtcacg tgcacgaggc cgtcgatgtc
     1921 gaggccgtcg agcgtcacga agatgccgaa gctcgcgacg ccgctgatcg tccccgggaa
     1981 cgtctcgccc accttctcct gcatgtagtg gcacttcaac cactgcagca cgtcgcgcga
     2041 tgcgtcgtcg gcgcggcgct cggtcatcga gcagtgcacg ccgagctcgg cccacgtcgc
     2101 atccttcggc gtgtaataag gtccccccac gctcgcggct ggcgccgctt cgctgccccc
     2161 ggagggggcg gattcgcgcc ttggggcggc cccgcggcgc gaaggctccc ccacgctcgc
     2221 ggctcgcgcc gcttcgctgc ccccggaggg ggcggattcg cgccttgggg cggccccgcg
     2281 gcgcgaaggc gttcccgcga gcaccgcctt gatcgcgcga tggacgagga ggtcgggata
     2341 gcggcggatc ggcgacgtga agtgcgcata ggcgtcgtac gcgaggccga agtggccgac
     2401 gttgtcgggg ctgtattcgg cgcgcgaaag cgagcgcagc aacacggtct gcaacagcgc
     2461 gaaatcgggg cgctcgcgaa tgcggtcgat cagcgcggcg taatccatcg ccgtcggctt
     2521 gtcgccgccg gtgaggctca gtgcgcagct cgcgaggaat tcgcgcagga cggcaagctt
     2581 ttcgggcggc ggaccctcgt ggacgcggta gagcgccggc tgcccgttct cggcgaggaa
     2641 gccggcggcg cacacgttgg cggcgagcat gcattcctcg atcagccggt gtgcgtcgtt
     2701 acgcggtgcg ggaacgatct ccgcgatctt gccgtacggg tcgaactgca gcgcgagctc
     2761 cgcggtgtcg aaatcgatcg cgccgcgccg ctcgcgtgcg gcgaggagca ccttgtagag
     2821 cgtgtagagc tgcgcgagat gtggcagcag cacttcgccg gccgtgcctt tcgcggcagc
     2881 cgtgtcgttg agccacgtgt acacctgcgt gtacgtgagc cgcgcgcgcg agtgcatcac
     2941 cgcggggtag aacgcgtacg atttgatcgt cccgcgggcg tcgatcctca tgtcgcatgc
     3001 catgcagagg cgctcgacgt cgggcttcag cgagcacagc tcgttcgaca gtgcctcggg
     3061 cagcatcggg atcacgcgcc gcgggaagta gaccgacgtg ccgcgttccc gcgcctcgcg
     3121 gtcgagcgcg tcgccgtcgc gcacgtaatg cgcgacgtcg gcgatcgcga cgatcagccg
     3181 gaagccgcgc ccgtcgggct cgcaagacac ggcgtcgtcg aagtccttcg cggtctcgcc
     3241 gtcgatcgtc acgagaggca agtgcctgag atcggtgcgg ctcttgcggt cggccggacg
     3301 cacttccgcc ggcaggcgca gcacctgccg ctttgccgcc ggattccact cgaacggcag
     3361 gtcgtgcttg cgcagcgcga tctcgatctc gatgccggga tcggtggcgc ttcccagcac
     3421 ctccttgacg cgcacgaccg cttcgccatg cgcgcttggc tgctccacga tctcgacgac
     3481 cacgacctgc gccggctgcg cgccgccgcg gtcgcccgca ccgagcagaa gatcctggtt
     3541 gatgcgccgg ttctcggcct cgacgaacca gatgccgtgt tcctcgtgga tgcgaccgac
     3601 gatctcgcgg ttggcgcgct cgagcacgtc gacgatctcg ccctccggac gcccgcggcg
     3661 gtcgacgccg atgcgcttcg ccgtgacgcg atcaccatgc agcaccttgt gcatttcgcg
     3721 cggggacagg aagagatcgt cgccgccgcc ttcggggacg agaaagccgt agccgtcggg
     3781 gtggccctgc acggtgccgc ggatgaggtc gagcttggcg acgatgcaaa gctcgccctt
     3841 gcggttgacg aggatctcgc cgtcgcgctc gagcgttgcg acgcaggcgt cgaacagatg
     3901 ctgctggcgg cggccgatcg acaggcgcga cgcgatctcg gcgggcgcga gcggaatgcc
     3961 ggcttcggtc agctcgcgca ggatcgcgtc gcaggtcggt ttcgattcgc cgtgcgccgc
     4021 cgcttcgggc gtacgcgggc gctcggagct ccgcggcgtg gttgcggcac gacggcgaag
     4081 gggaatcgtc tttgacaagg ctatgcgcag cttttaaaat taagggttcg tcacgcctgc
     4141 ccggatggcg gaattggtag acgcaccagg ttcaggtcct ggcggtggca acactgtgga
     4201 ggttcgagtc ctcttccggg caccatcgag caagccggcg cagtcgccgg tttttctgtt
     4261 tcttgaccca tcccgcatca tcatagccgg tgcccgatga gcgcggcgtg cgcgatgcgc
     4321 ccaagcgttc gagcgcccga aggtgcagga agtgcccggc gccgcagatc gcggcggatg
     4381 gacggcggga gcccggcatg gcggcaggcg aggtgacggt cgatccgcgc gtgcgtcgcg
     4441 gcttgccgct tttcgtcgtc cccgtgctgt gcgccgcggc gatgtacttc cgcttcttcg
     4501 ccggctactg gctcggcgac gatgtcggca acctcgaccg cgcctggctc gccgatcagt
     4561 cgggcgagct ctggcatcag gcatggacgc aattgtcgag cggcgcgtcg tcgcagggcg
     4621 cgttctatcg gccgcttatg cttgcgagca ttctcgtgaa cgggtggctt gcgggaagcg
     4681 tctacgtcgg ctggttcgcc gtgagcatcg cgatccatct cgccaacgtg gcgttcatcg
     4741 gcatgctcgt gtcgcgcctc gcacacgcat gcggtcgcaa cgggcgcgtc gcgggcgccg
     4801 tggccgcgct gtttttcgcg ctgtgtccgc tgctcgccga gggcgtgttc tggatatcgg
     4861 cgcgcgcgga cgcgttcgtc acgctgctca cgttgtcggc gttgtttgcg tggtcggtat
     4921 cgcgaacatc ggttggccag gcactgtggc cgcccttgct gctggccgtg gcactcgggt
     4981 tcaaggaatc ggccgcggta tttgcgttgc agatgctgct cgtcgcgctc gcctggccgg
     5041 tgcgcacgac gcgcgcgcaa cggatcgcga tcgctgccgg cttcgcgctc gtcgcgatgt
     5101 tcctcgtgct tcgcggccac ctgttcggcg acgcgtggcg cgtttactac gcgggcgccg
     5161 gcacgccgtc gctcgatcgg ctgcgcgaag cgatcgtgtc gcttccgccg tggtggtccg
     5221 cactgacgca ttcgacgccg catgttgcga acgtctacgc ggcgctcgca gcgcttgcgt
     5281 cggtggcact cctgttcgcc gtcacgtcgc actcgagtgc cgcgcggctc gtggcggcgc
     5341 ttctcgcgtc gtcggcgggc ctcgttgcgg cgacgttcct caatctcggc agcatgggtg
     5401 acaccggcga gggtggccgg ctcgcctaca cgccgatcgc gtggttggca ctcgcactcg
     5461 gcgtcgcggg tgccgctccg gtcggcgccg ttgaacgacg cgcccgcggc gaagcggcgc
     5521 gtctgccggg catcggactc cttttgctcg cgtgcgtagc gggcagctgg gtgctcgaag
     5581 gcgagctcgc gggcgcgagc cgcgccgagc gcggcatgcg cgcgcttgca gcgtcgattc
     5641 ccgactgggc gcgcagcaat ccgggactga cgctcctcgt gatcccggag cacgacggac
     5701 cggtgatcac cggacgcaac gcgcaggcgg ggctggtgat gccgccactg cagcgcgagg
     5761 cgctgctgca ccgggtattg cccacggtgc cgcgcgaagt gcaggcacgt tacgatcagc
     5821 tcgcggccgg gcttgcatcg cgtctcgcaa cgcttcgccc gtcccgcgtg gacgcgccaa
     5881 tgctcgcccg catcttcgcg ccggatacgg cgcgctggcc cgatcgatac gcgtgctggt
     5941 cgcccggcaa gcgacggatc gtcgaattgt cggcgccgaa tccggaagac cgcgcgcgat
     6001 gggtcgatga gctcgagcag gcggccgagc aatgcccgcc acgcagatga acgcatcggc
     6061 agcgaacgcg atgctgcttt gcgtcgctcg atgcgtggtg aggacgcgcg aggtcagtcg
     6121 cgctcgagcg ccacgccgag ccgatccttg agcgacgccg ggtcgagatc gtcgtacacc
     6181 tcgaacggct ggcggatcca cgggctgtcg ggaaggtgtt cgacgtagta gtcgggctcg
     6241 aacaccgagc atgccttcca ccacagcacg gccgtgtgca gttccgagat gtgcgggaag
     6301 cgccgcggca gctcgtcgtg gacggcggcg agcgtatgcc cggaatcgac catgtcgtcg
     6361 acgagcagca cccgatcgcc gaggcgcggc ttcgtcatcg tcatgtgctc ggcgatcacg
     6421 agctcgccgc ggatcgtgcc gccctcggcg gtgtacgaat gcgtcgacag gatggcgagc
     6481 ggctggtcga agatgcgtga cagcacgtcg ccgacgcgaa ggccgccgcg cgcgatgcag
     6541 atgatctggt tgaaccggaa gcccgactcg tgcacgttga gcgcgagtct ttcgaccagc
     6601 gtgttgtagc gcggccagtc gatgtcgagc gtcatcgcgc gaacggatgg tgcacgagga
     6661 tcgtctcgtc gcggtcaggc ccggtggaga ccatcgcgat cggcacgccg gtgagcgcct
     6721 cgatgcgtgc gagataggcg cgtgccgccg cgggcagcgc cgcgaacgac ttcgcgccga
     6781 ccgtgctccc gctccagcct ggcagcgttt cgtagacggg ccggcagcgc gcgacctcgt
     6841 cggcgcccgt gggtatgaga tcgacggtcg catcgcctac ctcgtagtgg gtgcagatcc
     6901 ggatctgtgc catgccgtcg aggacgtcga gcttcgtgat gcactggccg ttcacgccat
     6961 tgagcgcgaa cgagcggcgt agcgccggaa tgtcgagcca gccgcagcgc cgcggccgtc
     7021 cggtgaccga gccgaattcg ttgccgcgtt tcgcgagcat cgcgccggtg tcatcggtga
     7081 gctcggtcgg gaagggaccg gtgcccacac gcgtcgtata agccttcacg atgccgagca
     7141 cgtagtcgat catgtgcggc ccgatgcccg cgcccggcgc agcggcgccc gcaacgcagt
     7201 tcgagctcgt cacgtacgga tacgtgccgt ggtcgacgtc gagcagcgcg ccttgcgcac
     7261 cttcgaacag cagcgattcg ccgcgcgccc gtgcgcgctg gatcgcggcc gcgacgtcgc
     7321 ccaccatcgg cgcgatcgcg ccgccgagcg ccagcacgtc gtctttcgtc ttcgcgaaat
     7381 cgaccggcgc gcacttgaag taatggacga gcgtgaaatt gtggaggtcg agcagctgct
     7441 cgagcttggc ggcgaaacgc gccgggtcga gcaggtcctg cacgcgaagc gcgcgccggc
     7501 cgaccttgtc ctcgtaggcg ggaccgatgc cgcgtccggt ggtgccgatc ttcagagcgt
     7561 cgccgagcgc cgcttcgcgt gcctggtcga gcgccacgtg gatcggcagc accaacggac
     7621 acgcgggcga gatcatgagc cgcgagcgca cgtcgacgcc ggcctgctcg agctcttcga
     7681 tctcctgcat caatgccgac ggcgacagca cgaccccgtt gccgatcagg atcgcgacgc
     7741 cttcgcgcag cacgccggac ggaatgagcc gcagcacggt cttgcgcccg ccgatgacga
     7801 gcgtgtgtcc agcgttgtga ccgccctgga agcgcaccac gccctgcgca ctctcggtga
     7861 gccagtcgac gatcttgccc ttgccctcgt cgccccactg ggcgccgatg acgacgacgt
     7921 ttctggggtc ggagacgact tttgttgtca ttcgcgctcg ctcgtgcacc gtcaaaagtc
     7981 gtctctgacc ctacttcggt tcgccgcgcg cgagcacgcc cgcgagttga cgaagatcga
     8041 gcgtgaagcc ggtcgccggg cgcgcacggc cgaacgcctt gccgatgccg tcgtagcggc
     8101 cacctttgcc gacggcattc ggctcgcccg ccgtgaacac cgagaagatc gcgccgttgt
     8161 gataatgata gccgcgaagg tcggcgagat cgatgtgcaa cgcgtcgacg agcggcgcgg
     8221 ccgtctcgac gagcgcttcg agcgaggcga gcgcgttggc gatcgacgca atgtcgggca
     8281 gccgctcacg tgcggtgcgc agaacctccg gtgcggggcc gtaaagggtc ggcaatgcct
     8341 cgagcgcgtc gcgccaagcc ggtggcaggt gacgcgcgag cgcgtgcaca cccggcccgt
     8401 ccttcgtgcg cagcgcctcg aacagcgcgc tgtcgtcgct ttcggcgagc ccggccgcac
     8461 tcgcgagcgc gcgataaacg cccatgtggc caagatcgag gtgcagtccg cgaacgccgg
     8521 ctgcggtgag cgacgaaacg agcagcccga tcacttcgcg atcgccgtcg gtccccggca
     8581 cgccgaagag ctcggcgccg acctgcacca cttcacgcgt ggcacccggc ccctccgcca
     8641 cggcgcgcac gacgctgcct gcatagcaaa gtcgcgtcgc gcccggctcg ttgagcagat
     8701 gtgcgtcgat cctcgcgacc tgcggcgtga tgtccgcgcg gatacccacc agccgtcccg
     8761 attgcggatc gacggtcttg aacgtgagaa gctcgaggtc gcggccgctg ccggtcagca
     8821 gcgagtcgag atgctcgatc agcggcggac gaacgagccg gtagccatgg tgcgcgaagt
     8881 gatcgagcag cgtgcgccgc aacgcctcga gtccggccgc ctcggcgggc agcacgtcgt
     8941 cgatcgcttc gggaagcagc cagttgcgca cggtgcgcat cagatcaggt agagggcgag
     9001 aagcccgatg ccgatggcga tcatgccgat gaagcgaagc tgcccgtcgg tgagctcggt
     9061 caaatgccgg aagccttcac gccaaacgcg aggtgcggca aacggcagca atccttcgag
     9121 gatcagcagc agcgcaagcg cggtccacag cgaatcgggc acgctggcgc tccgtcgcgc
     9181 ttatttggcc ggcgttcgag gcgccgtgcg gctcgtgccc gggtccttga agtactggaa
     9241 gaaatcggag ctcgggtcga gcaccatcag gtcgctcttg ctgcgaaacg tcgcccggta
     9301 cgcctcgagg ctgcggtaga acgaatagaa ctcggggttc tgcgtgtacg cctgcgcgta
     9361 gaccgccgag gccttcgcgt cgccgctgcc cttgatcttc tgcgcctcgc tgtatgcgtc
     9421 ggcgagaatc accgtgcgct ggcgatcggc atcggcgcgg atcttctcgg attcggctgc
     9481 gccgagcgag cgcaattcgt tcgcgacgcg ccgccgctcc gattccatcc gctgatacac
     9541 gggaccggtc acgtcggggg gcagctcgac gcggcgcagc cgcacgtcga tcacctcgac
     9601 gccgatcgac ttcgcatcgg cgttcgcctt ctgctgcgtc accgaagtga tcttgtcgcg
     9661 cgccgacgag atggcctcgt gcacgtcgtg cttgttgaat tcctcggcga ggttggcgcg
     9721 gaccgtctgc gaaagacgcc ggcgcgccgc ttcctcgtca ccctgcaccg agcggtagta
     9781 ctggcccacg tcggtgatcc gccacagcac gacgaagtcg acgagcagcg gcttcttctc
     9841 cgacgtcgtc acccgatcgg gctcgggatt gtcgagcgtc aggttcacct tgtcgtagaa
     9901 cttgacgttc tgcacgagcg gaaccttgaa gtagagcccg gccgtcgtct tggtctcgac
     9961 gatctcgccg agctggaact tgatcgcgta cttgcgctga tcgaccgtgt agaccgattg
    10021 cgacgcgatg aacagcaccg cgacgagtgc ggcgaggagg ggcatcgacg atctcattgt
    10081 cccccccacg cttgcggctt cgccgctgcg ctgcccccca aggcgctcat cctcatctcc
    10141 cctcccgatt gcgcagcgca tcgcgcgagc gtgacgcatc gatcgacagc gcgttgtcgg
    10201 aaggcatcgc tcgcggcggc gtcgccgtgg cgtcgggcac ggcggcaccg ggcgccggcg
    10261 acgccgactg ctggatcagc ttgtcgaggg gcaggtacaa gaggcttccg ctcttctggt
    10321 cgacgagcac cttgctcgaa ttgccgagca ccgattgcat cgcatccaga tagagccgct
    10381 cgcgggtgac gccgggcgcc ttctggtatt cgaccagcac ctggttgaag cgctgcgcgt
    10441 cgccctgcgc gcgctgctcg acttcggatt tgtagccgtt cgcctcctcg agcagccgcg
    10501 ccgccatgcc gcgcgcacgc ggcacgacgt cgttggcata cgcctggcct tcgttcttga
    10561 gccgctcgcg atcctgtccc gccttgaccg cgtcgtcgaa cgcggcctgc accttgtcgg
    10621 gcggctgcac gttctgcagc gtgaccttct gcacgaacac gccggtgccg aagcgatcga
    10681 ggttctgctg catcagttgc tcggtctgct tcgcgatctg ctcgcgtccc tcgtagagcg
    10741 cgaagtccat cttcgccttg ccgacgacct cgcggatcgc cgattcggcg acatacgcga
    10801 cgatctggtc gggacgccga ttgttgaaga cgtagtcctc tgcgctcttc aggttgtatt
    10861 ggacggcgaa ctggatgtcg atgatgttct cgtcatcggt cagcatcacc gcttccttgt
    10921 cgatcttgtt cttcggcgtg ttgcggtagc cgacctcgat cgtcttcacc tgcgagaagt
    10981 cgacgagctc caccgtctcg atcgggaacg gcaggtgcca gcgcggaccg ggctgcgttg
    11041 tctcggtgta tttgccgaag cgcgtcacga cgccgcgccg gccttcgtcg acgatgtaga
    11101 agccgcttgc gagccagatg acgacgacga gcacgatcag catgccggct ccgccgagcg
    11161 gcaggtgcat gccgccaccg ccgccatcgc cgccgtccgg cgattcgcgg cggcgcccga
    11221 agagctcgtt catccggcga ttgacgttac gccagatttc gtcgaggtcg ggcggtccgc
    11281 tgttgttgct tcgcttgccc cactgcgggt cgttcagcga catacggatt ccactcgggc
    11341 tcggagcatg caaagttcgg cgcaaaggcg cccatggaac ccggaggctc acaggccgga
    11401 acgcgccggc gtgtcggttg gttcggaaac ggggagcggc gaggccggga agcgttcggc
    11461 cagcgcggcg cgcaactcgg cgatcccggc gccggtgacc gcactcacgc ggaccgacga
    11521 gatggtacca cagggggctc gctcgacgcc cggcacggtg ccggcgatat cgctcttgtt
    11581 gtagacgcga atctgcggca cgtcgcctgc gccgatctcc tcgatcagcg cgtcgacggc
    11641 ctcgatctgc tcgtcgcggt tcgcagcact cgcgtcgatc acgtgcagca gcagatcggc
    11701 atcggccgcc tcggccagtg tcgcgcgaaa cgccgcgacc agatcgtgcg gcaggtcgcg
    11761 gatgaagccg accgtgtcgg acagcacgat gttctcggcg ccttggacgt gcacgcggcg
    11821 cagcgtcgtg tcgagcgtcg cgaaaagctg gttcgctacg tagaccttcg ccgccgcgag
    11881 gcggttgaac agcgtcgact tgccggcgtt ggtgtagccg acgagcgcca cggtgcgcac
    11941 cgcggcacgc cggcgggcgc gattctgcgt ggcgcggctt cgcgcgacgc ggcgcaggcg
    12001 ctcgcgcagc agcttgacgc gctgcgtgat gaggcggcgg tcggtttcga gctgcgtctc
    12061 gccggggccg cgcaagccga tgccgccctt ctgccgctcg aggtgcgtcc atccaccgac
    12121 gagccgggtg gccatgtgat tgagctgtgc gagctcgacc tgcagcttgc cctcggcact
    12181 gcgcgcgcgt tgggcgaaga tgtcgaggat gaggctcgtg cggtcgacga cgcgacattc
    12241 gagcgcctgc tcgagattgc gctgctgcac gccggagagc gtgtggtcga agatgacgag
    12301 atcggcgtcg gcctcgcgcc gctgcgcggc gatttcgtcg accttgccgc gtccggcgaa
    12361 aagagccgcg tcgggacgcg gccggcgccc gcgaaggttg ccgacgaccg tcgcaccggc
    12421 cgatgcggcg agcgccgcga tttcggcgag ccggtcggac ccgtccaccc ggccgatgtc
    12481 gagcgcgacc acgagcgccc ggtcgccgat ttcgggacgt tccagcatgc cggctagctt
    12541 accgcgagcg gacgccgcgc cgcgcgcgcc gcaaccgcat gcgaaccggg ccggccggcg
    12601 tcagctttcg gcttcgtggt gattctgatg cggcatcgcg acgggccgcg acggcacgac
    12661 cgtcgagatc gcatgcttgt agaccatctg cgtgacggtg ttcttgagca gcaccacgta
    12721 ttggtcgaac gattcgatct gcccctgcag cttgatgccg ttcaccagat agatggaaac
    12781 ctgcacgtgc tccttgcgca gggtgttcag aaacgggtct tgtagcattt gccctttatt
    12841 gctcatcggt gagctccgcg cttgtttttc tggttaccga ctggaattcg cgtagggatt
    12901 ggcacctgtc ttgaattgta tcctaagggg tgtgccccgc aattggaacg cgtcgcggaa
    12961 aaaatgttcc agatagcggc ggtagccgtc gggcacgtgc gacagcgccg aaccgtggac
    13021 gatgacgagc ggcggattga cgccgccctg atgggcatag cgcagcttcg gccgcacgta
    13081 gcccgcacgc ggcggctgct ggcgcaggac ggcaagctgc agcgtgcgcg tcagccgcgg
    13141 tgtcggcagc cgcgccatcg ctgcagcgta cgcaaggtcc accgcgcgca gcaacgcctc
    13201 gacgccgcgt ccctcgcgtg ccgagatgta gtgatgctcg gcgaagccca ggaatgccag
    13261 cttgcgctcg aactcgcgct tgaccgttgc acgctgctcg ttgtcggccg cgtcccattt
    13321 gttgatgccg atggtcatcg cgcgccccgc ttcgaggatg tgtccggcga ggtgcgcatc
    13381 ctgctcggtg atgccgtcgc cggcatcgag cagcaggatc acgacgttcg cgtcctcgat
    13441 cgcctgcagt gtcttgatca ccgaaaactt ctcgacgact tcgctcacct tgccgcgccg
    13501 gcgcacgccc gcggtgtcga tcagcgtata gcggcgatcg gcgcgctcga aatcgagatg
    13561 gatcgaatcg cgcgtggtgc cgggttggtc gaaggcgatc acgcgctcct cgccgagcag
    13621 cgcattgact agcgtcgact tgccgacgtt cggccggccg acgacggcga cccggatgcg
    13681 ccgctcgccc tcgtcgagcg gctcggcctc ggcgggcggc gggcaatgct cgagcgcgat
    13741 gtcgatgagg tcgcgcacgt tttctccgtg cgcagccgaa atgggcagcg gttcgccgac
    13801 accgagctcg tgaaactcga cgctgcgctc gggcgcgagt ccttccgcct tgttgaccgc
    13861 gaggacgacc gggcgccccg acttgcgcag cagcgacgcg atcgcatgat cctgcggagt
    13921 gaccccctcg cgcgcatcga cgatgaagac gatcacgtcg gattcggcga tggcggcccg
    13981 cgcctgcttc gccatctcga acaggatgcc ggacgtggcg accggctcga agccgcccgt
    14041 gtcgacgacg agaaattcgc gatcgcctgc gcgtgcgcgt ccgtagtgac ggtcgcgcgt
    14101 caagcccggg aaatcggcga cgagcgcgtt gcgcgtcttc gtcagccgat tgaagagcgt
    14161 cgacttgccg acgttgggac ggccgacgag ggcgatggta gggagcatga gggtcagaga
    14221 cgactttccg gacgagtcac cgggttgccg ctcgatggtc gctgaaagtc gtctctgacc
    14281 ctacatcggt tcaacgagca gacaccgaaa agagcgtccc cttctccgat tgccacagca
    14341 tgccgccgcc gagcggcgcc ggttgcgtcg tggcgaggct gccgtcggtg gcaagccgcc
    14401 cgacatacgc gccatcgctg cgcgatagaa ggtgcagata cccctcgacg tcgacgacgc
    14461 cgatctcgtc gccgatcagt tgcggaccgc cgatgcggcg ctttccgaga acgtcctgct
    14521 tccacaccga cgcgccgttg ctccggtcga acgcgagcac gctgcccttg tcgtcgacga
    14581 cgtagacgcg attcgcgtcg gcggtgatgc cggcgaggct cgatacgtcg cgtgtccaga
    14641 tcagcgcgcc gcgcacgacg tcgaagcagg cgacgcggcc ctggtacgct gctgcgcagg
    14701 cttccttctc gtcgacgtag ggcaggctgg tcacgtcggc gatgcgctcg agctcggttg
    14761 cgccctttgg cgtcgcgacc gtgccgtccc acgcgacggt gccggttcgt gcatcgatcg
    14821 cgagcaggcg tccgcccgcg gtgccaacga acaggccgcc gcgcgacagg acgccgccgg
    14881 ccgcgttgcg cacggtcagt ggcggattcg tgcgcgggtc gacccacagc gtcttgccgt
    14941 cgctgaccga gagaccatag atgcgaccat cgccggagaa cacgacgacg acgtcctcgg
    15001 caacgcgcgg aggcgcgatc acttcgctcg acacgcgcac gttccagcgc gccttgccgg
    15061 cggtatcgaa cgcgagcacg tcgcccttgc tggtgccgac gacgacgagc gaggcatcgg
    15121 cccccggccc cgccgacagc gcctggccgg cgctgatctt ccattcgacg cgcccggtgg
    15181 caggatcgag ccgcatcagc gtgccgtcgc cggccgctgc gtagatggcc gacgcggtga
    15241 tcgcgggcgc gatcccgggc gcggccctgc cgacgctctg ctgccagtcg atgcgcggcg
    15301 tgacgctcgc gttgagcgtg ggcagcggcc ccggcttgtg gctgccgccg gtgagccacg
    15361 cgaacgaggg cacgggaatc gacgacatcc acgacgtcag cgacgattcg gaaaggctcg
    15421 cacagccggc gcacagcatg acggcgacga gcgcggccga tagagcgcga agcacgggca
    15481 tcgccgatgc cgctcgcgtc atttcaccgc tccgccggcc ttgccttgca ccgcgtcgcc
    15541 cgccgtggcg ggcgcggcct tcgacggtgc agttgccgcc ggcgcgttcg attcggccaa
    15601 cttcgccgtg gcaggaatcg cgtcgtgctt cacctgcacg taattgcgat acgccgattt
    15661 gggatcgagc ttggcgagcg cactctcgta ggcggcgcgc gcgtcggcgc cgcgtccggc
    15721 ggcggcgagc gcatcgccgc gaaggtcggc gaacatgccg tcgaacggcg cgggatgctt
    15781 cgcatcgagc gtcgccagcg cggtgtcgta ctgcttttcg tcgatctgga tctgcgcgag
    15841 acgatagcgc gcgatcgtct gcagctcttc ctcggaggcg tgatcgacga cccatgcgag
    15901 ctgcgccttc gcagcattgc gatcgccggc gtcgtagagc atcttggcgt agagcagctc
    15961 ggcgcgcggc gcgtagccgg tgctcgcgta gcggtcggtg atctgcgcga tggcgtcctt
    16021 cgccttcgcc gcgtcctttg tgcgtgcggc ctcgctgacg gcgctgtaga gcaccgacgc
    16081 gttctccgcg cggtggcctg cgtaccagcg ccatccctgg atgccggcga cgaccagcag
    16141 tccgagcacg agcgcaaccg tgacggttcc accgtagcgg ttccaccacg ccttcaggtc
    16201 gtcgatctgg tcctgttcct cgagatcata gagagccata ttcatcctta ccggtattga
    16261 gtgcggcgaa gcgggcgacc acgtcggcaa gcggcaccgc gacctggtcg ccgccttcgc
    16321 gcagctgctt gatcgcgacg gtgccggcgg cggcctcgtc gtcgccgacg atgatcgcga
    16381 gccgggcgcc gctcgcgtcg gcttttttca tctgcgactt gaagccgcca ccgcccgcat
    16441 gcagaacgat gctcgcaccg tggtcgcgca gcgtttcggc gacatggcgc gcaacttcgc
    16501 ccgccttgtc gcccgcgtgc acgacgtacg catcgagcgc cggcgcgtgc gccttgccct
    16561 gggcttcgag cagcaggatc attcgctcga cgccgatcgc gaatccgcac gccggcgtcg
    16621 gcttgccgcc cagttccgcg aacagcccgt cgtagcgccc gccgcccgcg acggtgccct
    16681 gtgcgccgag cgcgccggtg acccattcga aaacggtgcg attgtagtag tcgagcccgc
    16741 gcacgagccg cgcgtcgacc gtgtacgcga tcccggcgtc gtcgagcagg gcacgcaacg
    16801 cgtcgaagtg tgcgcgcgag gcgtcgccga gctggtcgac gaggcgcggc gcggcggcga
    16861 tgacgtcctg cagcggcggg tgcttgc
//
LOCUS       DASSVX010000034         5209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_951167, whole genome shotgun sequence.
ACCESSION   DASSVX010000034 DASSVX010000000
VERSION     DASSVX010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951167"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1232
                     /locus_tag="VFO53_01670"
     CDS             63..1232
                     /locus_tag="VFO53_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET8875010.1"
                     /translation="MQNLLDLEPAALAHWFAERGEKPFRARQVCRWVHRRFAADISAM
                     SDLAKPLRASLAGAAAIRAPAVLRDTTAADGTRKWLLDAGNGNAVEAVYIPEDDRGTL
                     CISSQAGCALDCAFCSTGRQGFNRNLTTGEIVGQLWHANRTLLAEAIRAEGPPRGANS
                     APPGGSAAAQRQAWGDHIRAPITNVVMMGMGEPLANFDNVVPALRTFLDDNAYGLSRR
                     RVTLSTSGLVPWIDRLRDECPVALAVSLHAPTDALRDRLVPINRKHPLASLMAACVRY
                     LERAPRDFITFEYVMLDGVNDQPEHAAALLALVRDVPCKLNLIPFNPFPDSGFTTSPR
                     ERVRGFANALQDAGIVATIRKTRGDDIDAACGQLAGQVRDRTKHRLAARIPLVAR"
     gene            1259..2062
                     /gene="pilW"
                     /locus_tag="VFO53_01675"
     CDS             1259..2062
                     /gene="pilW"
                     /locus_tag="VFO53_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HET8875011.1"
                     /translation="MKRLASPLLVVAAFALAACASKGPAPQQAAAPAPDTTPVKSQEA
                     TPQERAKIRTDLAAGYYQRGQMDIALEELGNAKALDPGYSKIYDIYGLVYAMLGERAK
                     AEENFRQALALAPDNSEIRENWGAYLCGTGRAREALPEFEQVLKDPLYRTPEIALINA
                     GKCSAAVGDTKNAEEYLRRALSASPGNPTAAYNLALIAYRDKRMGEARAWMVPVMRQA
                     SPSAQALYLGLCIERKQGDREAERSYESQLKNRWPESAEAKAAAGGACE"
     gene            2059..3063
                     /locus_tag="VFO53_01680"
     CDS             2059..3063
                     /locus_tag="VFO53_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HET8875012.1"
                     /translation="MNAPRTDPPPDANAKESVASARAQFTAGARLRAAREATGLSVDA
                     VARQLKLAPRQVKALEDDDWQHLPGRTFVRGFARNYARFVQIDPDAVIALLPAPDAAP
                     ALERPALAASRRPMGEIPVERVTKPSAARWLVPLLIILIIAAVAYFQLARSSFHLPAL
                     LSSLASHDTESEPGSAVPATAATPGTGSSALPNPVAQPGAAPVAPGVPAASTDAGTPG
                     NATRAVPGGAVTVLPTPRAADASAAAATTPASGGAAPAETPLVLTFKGTSWAEVKDAK
                     GRVVLQMTGGAGMTQTISGVPPLELSLGNARDVDVTFRGQSLDLAPYTRGNVAHVSLQ
                     "
     gene            3248..4561
                     /gene="ispG"
                     /locus_tag="VFO53_01685"
                     /gene_synonym="gcpE"
     CDS             3248..4561
                     /gene="ispG"
                     /locus_tag="VFO53_01685"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET8875013.1"
                     /translation="MEREAIVRRASRRVDVGGVSVGGGAPIVVQSMTNTDTADIAATV
                     EQVRALAAAGSELVRVTVNTTEAAAAVPEIRSRLDDSGCTVPLIGDFHFNGHKLLTEH
                     PACADALAKFRINPGNVGKGSKRDSQFATLIELAIRHRKPVRIGVNWGSLDQALLARM
                     MDDNARAPQPAEAEAVMREALVASALESAAQAEALGLPRERIILSCKVSNVQDLIVVY
                     SELARRCDYALHLGLTEAGMGSKGIVASTAAMAVLLQQGIGDTLRVSLTPEPDGDRTR
                     EVVVAQELLQTMGLRSFAPMVTACPGCGRTTSSYFQELAGHIQSFLRASMPAWRERYP
                     GVEAMHVAVMGCVVNGPGESKLANVGISLPGTGETPVAPVYVDGQKTVTLKGDRIAEE
                     FERIVVDYVRATYGGEAKSAALVPQQKTIAVKAMGSARSSGAVPR"
     gene            4593..>5209
                     /locus_tag="VFO53_01690"
     CDS             4593..>5209
                     /locus_tag="VFO53_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET8875014.1"
                     /translation="MGLALQAVRGMNDVLPDEAAVWQRFEDTARNVFERYGYRNLRVP
                     VVEPTPLFVRGIGAHTDVVEHEMYTFEDKLNGESLTLRPEATAGIVRAAIEHHLTYER
                     PQRIWTGGPMFRHERPQKGRYRQFHQLDVEALGFPGPDVDAEQIVMLARLWRALGLEG
                     ITLSINSIGDPAERRLHRDALIAHFERHAGVLDADSKRRLHANPLR"
BASE COUNT          849 a         1855 c         1756 g          749 t
ORIGIN      
        1 atcgcctatt tcttcccggc atacagcatc tacgcacggt aatcggagga cgacgcgtcg
       61 cgatgcagaa cctgctcgac ctcgaacccg cggcgcttgc ccactggttc gccgaacgcg
      121 gcgaaaagcc gtttcgcgcg cgccaggtct gccgctgggt gcacaggcgt ttcgccgccg
      181 acataagtgc gatgagcgat ctcgcgaaac cgctgcgcgc gtcgctcgcc ggcgccgccg
      241 cgatccgcgc accggcggtc ctgcgtgata ccaccgccgc cgacggtacg cgcaaatggc
      301 tgctcgatgc cggtaacggc aatgcggtcg aggccgtcta cattcccgag gacgaccgcg
      361 gaacgctgtg catctcgtcg caggccggct gcgcgctcga ctgcgcgttc tgctccaccg
      421 ggcggcaggg attcaaccgc aacctcacca ccggcgagat cgtgggccag ttgtggcacg
      481 cgaaccgcac cctgcttgcg gaggccattc gcgccgaagg gccgccccga ggcgcgaatt
      541 ccgccccccc ggggggcagc gcagccgcgc agcggcaagc gtggggggat cacatccggg
      601 cgccgatcac caacgtcgtc atgatgggaa tgggcgagcc gctggcgaac ttcgacaacg
      661 tcgttcccgc gctgcgcacg ttcctcgacg acaatgccta cggactttcg cgacggcgtg
      721 tcacgctgtc gacgtcgggc ctcgtgccat ggatcgaccg gctgcgcgac gaatgcccgg
      781 tcgccctcgc cgtgtcgctg cacgcgccca ccgacgcact gcgcgaccgc ctcgtgccga
      841 tcaatcgcaa gcacccgctc gcgagcctga tggccgcgtg cgttcgctat ctcgaacgcg
      901 cgccgcgcga tttcatcacg ttcgagtacg tgatgctcga cggcgtcaac gaccagcccg
      961 agcacgcggc cgcgctgctt gcgctggtgc gtgacgtgcc atgcaagctc aatctgatcc
     1021 cgttcaaccc gttcccggac agcggcttca ccaccagtcc gcgtgaacgc gtgcgcggtt
     1081 tcgcgaacgc attgcaggat gccggcatcg tggcgacaat ccgcaagacg cgcggcgacg
     1141 acatcgacgc cgcgtgcgga cagcttgccg ggcaggtgcg cgatcgcacg aagcaccggc
     1201 tcgccgcccg cattccgctc gtcgcgcgct gaacgcgcat cacctcggac atcgccgcat
     1261 gaaacgcctt gcatcgcccc tgctcgtcgt ggctgcattc gcgctcgccg catgtgcgtc
     1321 gaagggtccg gccccgcagc aggcggcggc acccgcaccc gacacgacgc ccgtcaaatc
     1381 gcaggaggcc accccgcagg agcgcgcgaa gatccgcacc gatctcgcgg ccggttatta
     1441 ccagcgcggg cagatggaca tcgcactcga ggagctcggc aatgcaaagg ccctcgatcc
     1501 cggctattcc aaaatctacg acatctatgg tctcgtctat gcgatgctcg gcgagcgggc
     1561 gaaggccgag gagaatttcc ggcaggcgct ggccctggca cccgacaatt ccgaaatccg
     1621 cgagaactgg ggcgcctatc tgtgcggcac cggccgcgcg cgcgaggcgc tgcccgagtt
     1681 cgagcaggtg ctgaaggatc cgctttaccg aacaccggaa atcgcgctga tcaacgcggg
     1741 caaatgcagc gcggccgtgg gcgacacgaa gaatgccgag gagtatctgc gccgcgcgct
     1801 ttcggcatca ccgggcaatc ccaccgccgc ctacaacctc gcgctcatcg cctatcgcga
     1861 caagcggatg ggcgaggcgc gcgcatggat ggtgccggtg atgcggcaag cgtcgccgtc
     1921 agcgcaggcg ctctacctcg gcctgtgcat cgagcgcaag cagggcgacc gcgaggccga
     1981 gcgctcgtac gagtcgcagt tgaagaaccg ctggcccgag tcggccgaag ccaaggccgc
     2041 cgccggcggt gcgtgcgaat gaacgcccca cgcaccgatc ctccgccgga tgcgaacgcg
     2101 aaggaaagcg tcgcgagcgc ccgcgcacaa ttcaccgcag gtgcgcggct tcgcgcggcg
     2161 cgcgaagcga ccggattgtc ggtcgacgcg gttgcgcggc agctgaagct cgcaccgcgg
     2221 caggtgaagg cgctcgagga cgacgactgg caacacctgc ccgggcgcac gttcgtccgc
     2281 ggtttcgcac gcaactacgc gcgcttcgtg cagatcgatc ccgacgcggt gatcgcgctg
     2341 ctgccggcgc ccgacgcggc acccgcgctc gaacgcccgg cactggctgc atcgcggcgt
     2401 ccgatgggcg agattccggt cgagcgcgtg accaagccgt ccgcggcgcg ctggctcgtt
     2461 ccgctgctga tcatcctgat catcgccgcc gttgcctatt tccagctggc gcgctcgtct
     2521 ttccatctgc ccgcgctgct gtcgtcgctt gcgtcgcacg acacggaaag cgaacccgga
     2581 tccgcggtgc cggccaccgc tgcgacaccg gggaccgggt cgtcggcgct tccgaatccg
     2641 gtggcgcaac cgggggcggc gccggtcgcg ccgggagtac cggccgcatc gaccgatgcc
     2701 ggcacgccgg gcaacgcgac gagggccgtc ccgggcggtg ccgtcaccgt gctgccgaca
     2761 cctcgggcag ccgacgcatc cgcggccgca gcaaccacgc cagcgagtgg cggcgcggcg
     2821 cccgccgaga cgccactcgt gctcacgttc aagggcacgt cgtgggccga ggtcaaggac
     2881 gccaagggcc gtgtcgtgct gcagatgacg ggcggtgcag gcatgacgca aacgatctcc
     2941 ggcgtgccgc cgctcgagct ctcgctcggc aacgcgcgcg acgtcgacgt cacgttccgc
     3001 gggcagtcgc tcgaccttgc cccctacacg cgcggcaacg tcgcgcatgt gtcgctgcaa
     3061 tgagcggcgt cgggccgtcc caagccgcga attgcgcccc ctccgggggc agcgcagcgc
     3121 gcgagcctga cgggccgccc cgagggcgag cagctgagcg cgaagcgcga agcccgtcca
     3181 acccgccgca agcgtggggg gatcatacaa tccccatcgt gcaactcccg gccgggccgg
     3241 catcgtgatc gagcgcgaag cgatcgttcg ccgcgcaagc cgccgcgtcg acgtcggcgg
     3301 cgtttccgtt ggcggggggg cgccgatcgt cgtgcagtcg atgacgaaca ccgacaccgc
     3361 cgacatcgcc gcgaccgtcg agcaggttcg cgcgctggcc gccgccgggt cggagctcgt
     3421 ccgcgtcacc gtcaacacga ccgaagcggc cgccgccgtc ccggagattc gcagccgtct
     3481 cgacgacagc ggttgcacgg tgccgctgat cggcgatttc catttcaacg gccacaagct
     3541 gctgaccgag catccggcgt gcgccgacgc gctcgccaag ttccggatca accccgggaa
     3601 cgtcggcaag ggcagcaagc gcgattcgca gttcgcaacg ctgatcgagc tcgcaatacg
     3661 ccaccgcaag ccggtgcgca tcggcgtcaa ctggggcagc ctcgaccagg cgctgctcgc
     3721 gcgcatgatg gacgacaacg cgcgcgcgcc gcagcccgcg gaggccgaag cggtgatgcg
     3781 cgaagcactc gttgcctcgg cgctcgaaag tgcggcgcag gccgaagcgc tcgggctgcc
     3841 gcgcgagcgg atcatcctct cgtgcaaggt gtcgaacgtg caggacctga tcgtcgtcta
     3901 ttccgagctc gcgcgccgct gcgactacgc gctgcatctc ggcctcacgg aagccggcat
     3961 gggcagcaag ggcatcgtcg cgtcgacggc ggcgatggcc gtgctcctgc agcagggcat
     4021 cggcgacacg ctgcgcgtgt cgctcacgcc ggagcccgac ggcgatcgca cgcgtgaggt
     4081 ggtcgtcgcg caggaactgc tgcagacgat gggcttgcgc tcgttcgcgc cgatggtcac
     4141 ggcgtgcccc ggctgcgggc gcacgaccag cagctatttc caggagctcg ccggccacat
     4201 ccagtcgttc ctgcgtgcat cgatgccggc gtggcgcgag cgctacccgg gtgtggaggc
     4261 gatgcacgtc gccgtcatgg gttgcgtcgt caacggcccc ggtgaatcga agctcgccaa
     4321 tgtcggcatt tcactgccgg gtaccggcga gacgccggtc gcgcccgtct acgtcgacgg
     4381 gcagaagacc gtcacgctca agggcgatcg catcgccgag gagttcgagc gcatcgtcgt
     4441 cgactacgtg cgcgcgacgt acggcggcga agcgaagtcg gccgcgctcg tgccgcaaca
     4501 gaagacgatc gccgtcaagg cgatggggtc ggcgcgaagc agcggcgcgg ttccgcgctg
     4561 atccgatcgc ctggatcgct tcgggaaaac ccatgggact tgcattgcag gccgttcgcg
     4621 gcatgaacga cgtgctcccc gacgaggcgg ccgtctggca gcgcttcgag gacaccgcgc
     4681 gcaacgtgtt cgagcgctac ggctaccgca acctgcgcgt gcccgtcgtc gagcccacgc
     4741 cgcttttcgt acgcggcatc ggcgcccaca ccgacgtcgt cgagcacgag atgtacacgt
     4801 tcgaggacaa gctcaacggc gagagcctga cgctgcgtcc cgaggcgacg gccggcatcg
     4861 tgcgcgccgc catcgagcac cacctcacgt acgagcggcc gcagcgcatc tggaccggcg
     4921 ggccgatgtt tcgccacgag cggccgcaga aggggcgcta ccggcagttt caccagctcg
     4981 acgtcgaggc gctcggcttc ccggggccgg acgtcgatgc cgagcagatc gtgatgctcg
     5041 caaggctctg gcgcgcgctc gggctcgaag gcatcacatt atcgatcaac tcgatcggcg
     5101 acccggccga gcgtcgactg caccgcgacg cgctgatcgc gcatttcgag cgccacgccg
     5161 gcgtgctcga cgccgattcg aaacggcgcc tgcacgccaa tccgctgcg
//
LOCUS       DASSVX010000035        18202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_851236, whole genome shotgun sequence.
ACCESSION   DASSVX010000035 DASSVX010000000
VERSION     DASSVX010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 18202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18202
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851236"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..2143)
                     /locus_tag="VFO53_01695"
     CDS             complement(89..2143)
                     /locus_tag="VFO53_01695"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET8875015.1"
                     /translation="MNENANPLLDFTGLPRFDRIRAGHVAAALDALLVEARDAVDRVA
                     SDAGPATWASVVEPTETIFDQLDRAWGAVRHLNAVVSTPAIRDAYNAALPKVTALYAE
                     IAQDERWFGRFRALAGAPAFATLDDAQRKVVDNALRDFRLGGAELSDSDKARFKAVQE
                     ELATLSAKFDDNVLDSENAWALYVDHATGLAGVPADVVSAARDDAEADGRAGWKLTLR
                     HPCYTPVMQYADDRSLRAAMHRASATLASDLGASPGWDNTGVILRILELRREEARLLG
                     YASYAELSLVPKMAQTPAEVLAFLRDLAQRARPFAEREYDELRTFARDALGIDDCAAF
                     DLAYVAEKLKARRFAYSEQEVRQYFPEDRVLAGLFHVVETIYGVTVHPADAPAWHPSV
                     RFFDIHDARAGLVGQFYLDLYAREGKQSGAWMDDAINRRHVHDRLQHPVAYLTCNFPA
                     PVNGGPAYFTHDDVTTLFHEFGHGLHQLLTRVDVAGVSGLQGVEWDAVELPSQFMENY
                     CWQWDVLSQMTAHAQTGAPLPRELFERMLAARNFGSGMAMLRQVEFALFDMLVHFDHV
                     PAGEDGYAGVQEVLDAVRREVAVVPKPSYDRFMHAFGHVFAGGYAAGYYSYKWAEVLS
                     ADAFGLFEEAGVLSPTIGARFRDEVLARGGSRPALESFVAFRGRPPSLDALLRHNGMI
                     AS"
     gene            complement(2160..2858)
                     /locus_tag="VFO53_01700"
     CDS             complement(2160..2858)
                     /locus_tag="VFO53_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET8875016.1"
                     /translation="MPLAGAMILAAGRGERMRPLSDATPKPLLRAGGKPLIVWQIEAL
                     ARAGFRDIVINVAHRAQDLIDALGDGAALGVRLRWSREAEPLETAGGVATAIDLIAPG
                     PALIVSSDIVTRFDYASLQSRIAAMRDAPQASSRVHLVMVPNPAWHAAGDFALAGGRV
                     ELDGAKRLTFGNIGVYDTALFRELPRGVKLKMLPLYRDWIGRRIVSGERYDGRWANVG
                     TPADLAALDAALSR"
     gene            complement(2861..3880)
                     /locus_tag="VFO53_01705"
     CDS             complement(2861..3880)
                     /locus_tag="VFO53_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET8875017.1"
                     /translation="MPDARAAALAQWLAAVAPPVAAIAPASADASFRRYFRVTLAAPW
                     REVAGAATLIAMDAPPPNEDCRPYVHVARLMRDGGVNVPRIVAANIAEGYLLMSDLGS
                     ATYASVLDEENAGRLYADALDALVLMQRMRCEDDLPPYDDALLRRELALFPDWYVARH
                     LQRTLDASERASLERVFDVLIANNLAQPRVFVHRDYHSRNLMVTAPNPGVLDFQDAVH
                     GPITYDLVSLLRDAYVEWSEEQELDRAIRYWERARKAGLPVSTDFAGFWRDFEWMGAQ
                     RQLKVLGIFARLKHRDGKAGYVDDMPRVMRYLRRAAGRYRELEPLLALLDALENRAPG
                     YVASL"
     gene            3993..6341
                     /locus_tag="VFO53_01710"
     CDS             3993..6341
                     /locus_tag="VFO53_01710"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HET8875018.1"
                     /translation="MKLRAGPIVAWVAFTGALCAQTANAEPLSLKLSREIEAPAPAPV
                     SVTPGTLVAPLPAPATGEQEVRGALFLRADRVEGDDKRITATGNVELRSRRETVLADR
                     LSYDVDNQTIFGDGNVVLRRNFDWITGPELQYKRDTEVGFFRKPRFFIAETDGHGDAS
                     QIRFTGPNHYEASDASYTTCIAADPDWYLRGDELEVDNLRKVGTAHRVSVHFLGAPVL
                     YAPWLQFPLSNERKSGFLTPTLGSTGVRGFEVTTPYYLNLAPNYDATITPRLMTKRGL
                     MLGTQFRYLVGDATAPLGTANGEIDAEILTNDRQTGERRYALAWAHNEQVTPWLYGYW
                     NVNRVSDDKYFADFADRIAITSQKTLPREGGLVATSGPWTVSARVQSFQTLQDPNAPV
                     IPPYNRVPQILASLRDTDWLGLTWSGTGEYARFAQEALVPTGERIVAYPTVAWMRQGS
                     AWFFNARGGVHMREYLLDQTTPALPDRHPSYAIPIASIDTGLVFERDLDVVKTPLTQT
                     LEPRAFYVYIPHRQQTNAPVFDTALDDFNFSQLFAENRYIGNDRIGDANQLTLALTSR
                     FLDRDTGAERLRLAVGQRFYFEDQKVSLNEPLRSASTSDFLVSAEGRLSNAWSLTSLL
                     QYNFDAHEVERLNAGVRYTPTAGSVLNATWRYTRTIVDPTGAIGEIKQVDLSGQWPVG
                     GRWNLIGRWNYSFTDRKTLEAVAGIEYNADCWVLRVVGQRLTTTTQQATTSVFVQLEL
                     NGLARVGTSPLELLRRSVPGYVPTNDPSLRERDRRLEALPEF"
     gene            6356..7705
                     /locus_tag="VFO53_01715"
     CDS             6356..7705
                     /locus_tag="VFO53_01715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET8875019.1"
                     /translation="MAARHSYDHALRRPSALQWALLLLVLFAAFAFMQPLAHAAANPV
                     PLDRVLVIVNDEAITQWDMSEQRRILLSQMQASKITPPPNDVLDKQVLERLIVERAVL
                     QYAKETGIRIDDTTVERTILRVAEENKLSPDEFRKVLEREGIPYANYREDIRRQITIQ
                     RVRDREVDSKVTVSDGEVDNYLATVASQAGGEDEYLLSHIYISVAEQASPTAVDESRK
                     RAEQALAEVRSGKDFGQVAASYSSAPDATSGGNLGWRTRARLPSVFADIVQKMKPGEV
                     SAVLRSASGFHVVKLVEERNRNQPTVVDQTHARHILIKVNEQTSESDAKIKIDRLRER
                     LVAGANFEELARANSEDASNAKGGDLGWLSAGDTVPDFEHAMDRLAVNEISQPVRTPF
                     GWHLIQVIERRKQDVTQQRRRDEARKAIRQRKSDEQFEEFVRQLRDRTYVEYKTDDR"
     gene            complement(7738..10038)
                     /locus_tag="VFO53_01720"
     CDS             complement(7738..10038)
                     /locus_tag="VFO53_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875020.1"
                     /translation="MIVKRSSLPNPPAPGTPDTIGWLDTPFGAAIGDGERIRFAGWAL
                     SIHGIRAIEIRFADRTQVARYGMPRPDVAAVYPGYPARAYCGFEATVRALGPLPNLVA
                     RGAVRVIAIAGDGSETTLGRLALVNPAAHERWSFLAKRPARPFFLLPATSGVSAGGAN
                     ELERAYAAYVSPTTKIGMRVPILYLRTTTGVAGDYRFDPDFDVRRRNGARAVADDALA
                     PVLTHSAQHRLPVLVTLNGGIWADASGTCPAWDVNDRLEEDVANCQWNERNEVMPDDH
                     LSHLPGSQKAPELARALTLNVYARAVRHYKRRNLQAAAAHLAGFMRAHPDLVVGVNLD
                     PDVYINPFFSGSQWYDYNPGTLRQFRHWLAGSGPYAGACEADVPDLSSYRRRHPLTLD
                     EVCAVAQRRFASFEAVDPPRRFVHGTDHPHWKDPWVTEWEVFRRHLVRLHYDELARWV
                     CDAGIPRGCIWSSQGLDAPSDLHAPPEGFMPLALRESSAPRNFDSGGVCIEGSKPRDG
                     HLGAIVYGAAATNDVPMENGRSLYATLASVDADFGIVEFNTADFRHPERQPTYASAYR
                     ALRALWNQGARFVSPMAWNGSNGAHAHDAEYVTFTAWRNTPLEEAACDFLLERAGLPA
                     GSRLWTFGTPVHADDDGWTLDAGSMRALPGGLALTADRRGRVTLVSPPEVALRCGDID
                     AVVAGLPGDVAPEAIRIDARGANGARWQQLVDARASSLACTAAGRVIAIPRITRAIDV
                     DQLRMTIRVAPCAQFTLERVAILRRT"
     gene            10260..11273
                     /locus_tag="VFO53_01725"
     CDS             10260..11273
                     /locus_tag="VFO53_01725"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8875021.1"
                     /translation="MAIDGARVSAAGWSMPVDDRAPEEGWFTINGRSFDRIAYPLPRA
                     DVGAVFWQREGSALCGFQCEIDDAGDPYPSGLMEIRRAIASTPCIERGRDSWFIPDPA
                     RHANLPDEDRRFRVIGDRDARGFLVSGATDYQRIDRVLVEVAGRHLHEFAGVLDWGVG
                     CGRVARHFPAAHARALTGCDIDRDNVDWCAANLPGTFAASRLVPPLPFEDARFDVVYG
                     VSVFTHLREPMQLAWLAELSRVVRRGGLVLTTTHGETAIDFARLSPLDYRQTRARVAR
                     EGLVVSGSNTQLDGHADHEGEYVNVYHSADYVQRTWSRHFDVLHIVPGYILHHDLVVM
                     RKP"
     gene            11270..11998
                     /locus_tag="VFO53_01730"
     CDS             11270..11998
                     /locus_tag="VFO53_01730"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8875022.1"
                     /translation="MNTDSPADRPLIDDQIAYYRARAPEYDAWWLRTGRFDRGAGNNA
                     AWHAEVAIVEGAVAGMLAKARPSSVLELACGTGLFTRHLAPRVGSVTAVDASPEVIAI
                     NRGRVGAPNVRYVEADLFAFEPQMRYDCVFMSFWLSHVPHARFDAFWSMVRRALAPGG
                     VAFIADSAHDPTSHAVDHPTPDRQAGIVTRRLDDGREFRIVKVFWEPAKLVARLAACG
                     FAAAIAGTPRYFIYGEARVAGDAG"
     gene            complement(11962..14025)
                     /locus_tag="VFO53_01735"
     CDS             complement(11962..14025)
                     /locus_tag="VFO53_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875023.1"
                     /translation="MGVRRAGRAASRWSAVVAVVVALAAAAQNAATPAPPPKRPIPPH
                     ALTLWSARLDAHPALARQPFYFLMMTSGIAAGGAREVDTEYRDYLSRTQKVGVAVPIL
                     YLRTTRGTRGDWVFDPAFDTKRLCGTRPVAEDNLKSVIDYALAHRLPVQFILNGGIWS
                     DASCETPQWDLTDHLEQDKANVQWTQDNEAFPDDYLKNLNGSTASPELARSLTYNVYA
                     SDVRRYKKRNLQAAARIVAAFAREHPDLFVGVVLDADTYMNPFFRGTQSFDYNPGMLR
                     QFREWLRVSGPYAGRPTDHAPDLSAYRRARPMTLADVNRIAGRQWKSWDQVEPPRRLP
                     GIVQPLAPGERPIWDDPWWNLWDRFRKHIVDLHYDELSSWAHAAGIPRERIYSAQGLI
                     HNDPELPPFAVRVDSDSASYDSAGVSVEGAIPRDGHLGAILYGRSARNAVVLEGGHSL
                     FATIGRMDDGWGIVEYNNTDLKFPQKKPDYAMAYQTFRDAFNNGAREVSAMAWNGSNG
                     IYADQPGYVPYTSWRNTPAEEAMRDFLVSHADVPGGARLWTFGTPVHVDADGWTAERG
                     TLTPGAGFVTLQPAAREVTLLSPPDQVIRPQRLASLALRFDANVKPSRMTVEAQEQPS
                     GPWRAVGATSGPELAFKWPASWNNSRTIVTRLRVSLSFPANAAGVRLARLLLYPASPA
                     TRASP"
     gene            complement(14027..14233)
                     /locus_tag="VFO53_01740"
     CDS             complement(14027..14233)
                     /locus_tag="VFO53_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HET8875024.1"
                     /translation="MPFLPARHYVSDHTRFIGDLLAKKPEIETEQRIGRRIWWDKLPS
                     DVEAQREMNAGRVPQKGYVYYALE"
     gene            complement(14268..15413)
                     /locus_tag="VFO53_01745"
     CDS             complement(14268..15413)
                     /locus_tag="VFO53_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HET8875025.1"
                     /translation="MTERFFAPCPRGLERPLAAELAALDAGDITPAEGGVGFAGETDL
                     AYRANLESRIASRVLWRVGSGPYRDERDLYALVHGIDWMRHFKPERTLRVDVAATRSP
                     LASLEFATLRVKDGVCDRFRSDCAVRPSVDKRAPDVRVHVHLTECDASIYLDTSGEPL
                     FKRGYRRDADEAPLRENLAAGLLALAQWTPERALLDPMCGSGTIVAEAAQIAADRAPG
                     LSRSFGFQKLSWYDGPAWQRIRQRAHDRVREAPATPPIVASDIAPAAIARTASALRNA
                     QVDAFVHVEAADVLTRAAPASSGLLLANPPYGVRLADAAQLAAFYPLLGNALKRRFSG
                     WTAGLFTGDLRLAKLIGLKPERRIPLWNGAIECRLFLFPLVEGSMRR"
     gene            complement(15426..15851)
                     /locus_tag="VFO53_01750"
     CDS             complement(15426..15851)
                     /locus_tag="VFO53_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HET8875026.1"
                     /translation="MYALVKTLHIVFMVSWFAGLFYLPRLFVYHAMTPADDARGIARF
                     KVMERKLYIGIMTPGAVLTIVFGLWLWLGFGVSGGWLHAKVALVLALIALHVYLGLLL
                     RDFARDGNRHGHAFYRWLNEIPALPLLVAIVWLVVAKPF"
     gene            complement(15883..16671)
                     /locus_tag="VFO53_01755"
     CDS             complement(15883..16671)
                     /locus_tag="VFO53_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875027.1"
                     /translation="MNTTSILRLPRLSVRFVPVWRRNLLVWRKLAIASVLGNIADPLL
                     YMLALGYGIGAMVGQVNGMSYVAFIGTGMVCQSAMFTSSFEGMYSAFSRMHVQRTWEA
                     IINAPIALDDVMLAEWMWCATKAVMSTAAILIVIVALGFGHTWLALWVLPLGFLVGLC
                     FGAFGLVMNSLAPGYDFFTYFFTLVLTPMLLFSGVYFPVDQMPAWLATIAAALPLKHA
                     IDVARPLMLDRVPDNIVLHVAVLAAYALVAYYAALSLTRRRLLK"
     gene            complement(16682..17647)
                     /locus_tag="VFO53_01760"
     CDS             complement(16682..17647)
                     /locus_tag="VFO53_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8875028.1"
                     /translation="MNGAALPEAVLTQAVLHDAPILRVDGLRKRYGTQEVVRGLSFAI
                     RRGECFALLGPNGAGKTTTLRCCLGLIDPDGGTIELVGEAVPKAAREARVRVGVVPQM
                     DNLDPDFTVRENLLIYGGYFRLPRSEVEERIPRLLDFAGLAGKAATSIRTLSGGMKRR
                     LTLARALINDPDLLILDEPTTGLDPQARHLIWDGLRQLLKQGKTILLTTHFMDEAERL
                     ATRLAVIDHGTMIACDSPRALLAQHVEPDVVEVYGEAAKTWAETRGRSLATRLELAGE
                     TAFCYATDAAPLLADLATHAGVRYLHRPANLEDLFIKLTGRELRD"
     gene            complement(17644..>18202)
                     /locus_tag="VFO53_01765"
     CDS             complement(17644..>18202)
                     /locus_tag="VFO53_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8875029.1"
                     /translation="PAVRGVALVCHPHPLQGGTLDNKVVQTLARTFFGLGYVAVRFNF
                     RGVGQSAGTFDEGDGETDDALAVLADARARFGDSLPVALAGFSFGSYVQTRVAERVVP
                     ERLVLVAPAVRRFAVRGVAAETIVIHGDEDDVVPLADVLAWARPQELPIVVFPGCGHF
                     FHGRLPQLSRMVAALWPPKSTEGSA"
BASE COUNT         3034 a         6149 c         6244 g         2775 t
ORIGIN      
        1 tgcgcaagcg caccgctcgc gagggcggcg atgacgacga cggacagcgt ggcgatcggg
       61 cttcgagcca tggcgaaggg ggaagcgctc aggacgcaat cattccatta tggcgcagaa
      121 gtgcgtcgag cgaagggggg cggccgcgga aggcgacgaa cgactcgagc gcgggacggc
      181 tgccgcctcg tgccagcact tcgtcgcgaa agcgcgcgcc gatcgtcggc gacagcacgc
      241 cggcctcctc gaagaggccg aacgcgtcgg ccgacagcac ctcggcccat ttgtagctgt
      301 agtagcccgc cgcgtatccg ccggcgaaca cgtgcccgaa cgcgtgcatg aagcggtcgt
      361 acgacggctt cggaaccacc gcaacttcgc ggcgcacggc gtcgagcact tcctggacgc
      421 ccgcgtagcc atcctcgccc gcaggtacgt gatcgaaatg cacgagcatg tcgaacagcg
      481 cgaactccac ctggcgcagc atcgccatgc cgctgccgaa gttgcgcgcc gcaagcatgc
      541 gctcgaacag ctcgcgcggc agcggcgcgc cggtctgcgc atgcgccgtc atctgcgaca
      601 gcacgtccca ctgccagcaa tagttctcca tgaactggct cggcagctcg accgcgtccc
      661 attcgacgcc ctgcaatccc gaaacgccag cgacatcgac gcgcgtcagc agctgatgca
      721 ggccatgccc gaactcgtgg aagagcgtcg tcacgtcgtc gtgcgtgaaa tacgccggac
      781 cgccgttcac cggcgccgga aagttgcagg tgagataggc aacgggatgc tgcaggcggt
      841 cgtgcacatg gcggcggttg atcgcgtcgt ccatccatgc gccgctctgc ttgccctccc
      901 gcgcgtagag gtcgagatag aactgcccga cgaggccggc acgggcatcg tggatgtcga
      961 agaagcgcac gctcgggtgc catgccggtg catccgccgg atgcacggta acgccgtaga
     1021 tcgtctcgac gacgtgaaac aggcccgcga gcacgcggtc ctcggggaag tactgcctca
     1081 cctcctgttc cgaatacgcg aagcgccgcg ccttgagctt ttcggcgaca taggcgaggt
     1141 cgaaggcggc gcagtcgtcg atgccgaggg catcgcgtgc gaacgtgcgc agctcgtcgt
     1201 attcgcgctc ggcgaacggg cgcgcgcgct gtgcgagatc gcgcaggaac gccagcacct
     1261 cggccggcgt ttgtgccatc ttcggcacga gcgagagctc ggcgtagctc gcgtagccca
     1321 gaaggcgcgc ttcctcgcgg cgcagctcga gaatgcgcag gatgacgccc gtgttatccc
     1381 agccgggcga cgcgccgagg tcggacgcga gcgtcgccga cgcgcgatgc atcgctgcgc
     1441 ggagcgaccg gtcgtcggca tactgcatca ccggcgtgta gcacggatgg cgcagcgtga
     1501 gcttccagcc tgcacggccg tcggcctcgg cgtcgtcgcg cgctgcgctg accacgtcgg
     1561 ccgggacgcc cgcgaggccg gttgcatggt cgacgtaaag cgcccacgca ttttcgctgt
     1621 cgagcacgtt gtcgtcgaac ttcgccgaca gtgttgcaag ctcctcctgc accgccttga
     1681 accgcgcctt gtcgctgtcg gaaagctcgg caccgccgag tcgaaaatcg cgcagcgcgt
     1741 tgtcgacgac cttgcgttgc gcatcgtcga gcgtcgcgaa agcgggcgcg ccggcaagag
     1801 cgcggaagcg cccgaaccag cgctcgtcct gcgcgatttc ggcgtagagc gcggtgacct
     1861 tcggcagcgc ggcgttgtat gcgtcgcgga ttgcgggcgt gctgacgacg gcattcaggt
     1921 gcctgaccgc accccatgcg cggtcgagct gatcgaagat cgtctcggtc ggttcgacga
     1981 cgcttgccca cgtcgccggg ccggcgtcgc tcgccacccg gtcgacggca tcgcgcgctt
     2041 ctacgagcaa cgcatcgagc gcggccgcga cgtggccggc gcggatgcga tcgaaacgcg
     2101 ggagtccggt gaagtccagc agcggattcg cgttttcgtt catcgcggtt gcaggtgctt
     2161 cagcgcgaca gcgcggcgtc gagtgccgcg aggtcggccg gcgtgccgac gttcgcccag
     2221 cggccgtcgt atcgctcgcc cgagacgatg cgccggccga tccagtcgcg atagagggga
     2281 agcattttga gcttgacgcc gcgcggaagt tcgcgaaaga gcgcggtgtc gtacacgccg
     2341 atgttgccga acgtcagccg cttcgcacca tcgagttcca cgcgtccgcc ggcaagcgcg
     2401 aagtcgcccg ccgcgtgcca cgccggattg ggcaccatca ccagatgcac gcgagaagac
     2461 gcctgcggcg cgtcgcgcat cgccgcgatg cgcgattgca gcgacgcata gtcgaagcgc
     2521 gtgacgatgt cggacgaaac gatcagcgcg gggcccggcg cgatgagatc gatggccgtg
     2581 gcaacgccgc cggccgtttc gagcggctcg gcttcgcgag accagcgcag gcgcacgccg
     2641 agcgccgccc cgtcgccgag cgcatcgatg agatcctgcg cgcgatgcgc gacgttgatg
     2701 acgatgtcgc gaaagccggc gcgcgcaagc gcttcgatct gccagacgat gagcggcttg
     2761 ccgccggcac gaagcagcgg cttcggtgtc gcatcggaca gcggccgcat tcgctcgccg
     2821 cgcccggccg cgaggatcat cgcgcctgcc aggggcatcg tcacagcgag gccacgtacc
     2881 ccggcgcgcg gttctcgagc gcgtcgagga gcgccagcaa cggctcgagc tcgcggtagc
     2941 gccccgcggc gcgacgcaga tagcgcatga cgcgcggcat gtcgtcgacg tacccggcct
     3001 tgccgtcgcg atgcttgagg cgcgcgaaga tgccgagcac tttcaattgc cgctgcgcgc
     3061 ccatccattc gaaatcgcgc cagaagcccg cgaagtcggt gctgaccggc aatccggcct
     3121 tgcgtgcgcg ctcccagtag cggatcgccc ggtcgagctc ctgctcctcg ctccattcga
     3181 cgtacgcgtc gcgcagaagc gaaacgaggt cgtaggtgat gggcccgtgc accgcgtcct
     3241 ggaagtcgag cacgcccgga ttgggtgccg tgaccatcag gttgcgcgaa tggtagtcgc
     3301 gatgcacgaa cacgcgtggc tgcgcgaggt tgttcgcgat cagcacgtcg aagacgcgct
     3361 cgagcgacgc gcgctcggat gcatcgagcg tgcgctgcag atggcgggcg acgtaccagt
     3421 cgggaaacag tgccagctcg cgccgcagaa gcgcatcgtc gtacggcggc aggtcgtcct
     3481 cgcagcgcat ccgctgcatg agcacgagcg cgtcgagcgc gtcggcgtac aggcgcccgg
     3541 cattctcctc gtcgaggacc gacgcgtacg tggcgctgcc gagatcgctc atgagcaggt
     3601 agccttccgc gatgttcgcg gcaacgattc gcggaacatt gacgccgccg tcgcgcatca
     3661 gccgcgcgac gtgcacatag ggccggcaat cctcgttcgg cggcggcgcg tccatcgcga
     3721 tcagcgtcgc agcccccgcc acctcgcgcc acggcgctgc gagcgtcacg cgaaaatagc
     3781 gccgaaagct cgcgtcggcc gatgcgggcg cgattgccgc gaccggcggc gcgaccgctg
     3841 cgagccactg cgcaagcgcc gcggcgcgtg cgtcgggcat gcgcgaaatc gggtccggca
     3901 tggtcacctg gggcatcgcg aatcggaaat cgaatggcga aacggctaga atgcgcaaat
     3961 tttagcaatc ccctgccatt gccttccact cgatgaagct tcgagccggc ccgatcgtcg
     4021 cgtgggtcgc gttcaccggt gcgttatgtg cgcagaccgc gaatgcggag ccgctgtcgc
     4081 tcaagctatc gcgcgagatc gaagcgcccg cacctgcgcc cgtgtccgtc acgccgggca
     4141 ccctcgtggc gccgctgccg gcgcccgcga cgggcgagca ggaggtgcgc ggagcgctgt
     4201 tcctccgcgc cgaccgcgtc gagggcgacg acaagcggat caccgcgacg ggcaacgtgg
     4261 agctccgtag ccgccgcgag acggtgctcg ccgatcgtct ttcctacgac gtcgacaacc
     4321 agacgatctt cggcgacggc aacgtggtgc tgcggcgcaa tttcgactgg atcacgggcc
     4381 ccgagctgca gtacaagcgc gatacggaag tgggcttttt caggaagccg cggttcttca
     4441 tcgccgagac cgatggccat ggcgatgcgt cgcagatccg cttcaccgga ccgaaccatt
     4501 acgaggcgag cgatgcgagc tacacgacgt gcatcgcggc cgaccccgac tggtacctgc
     4561 gcggcgacga gctcgaggtc gacaacctgc gcaaggtcgg gacggcgcat cgtgtgtcgg
     4621 tgcactttct cggcgcaccc gtgctctatg cgccgtggct gcagtttccg ctgtcgaacg
     4681 agcgcaaatc gggcttcctg acgccgacgc taggctcgac cggcgtgcgc ggcttcgagg
     4741 tgacgacacc ctattacctc aatctcgcgc cgaactacga cgcgacgatc accccgcggc
     4801 tcatgaccaa gcgcggcctc atgctcggca cgcagttccg ctatctcgtc ggcgacgcaa
     4861 ccgcgccgct cggcacggcg aacggcgaga tcgacgcgga gatcctgacc aacgaccgcc
     4921 agacgggcga gcggcgctac gcccttgcgt gggcgcacaa cgagcaggtc acgccgtggc
     4981 tctacggcta ttggaacgtc aaccgcgttt ccgacgacaa gtacttcgcc gacttcgccg
     5041 accgcatcgc gatcacgtca cagaagacgc tgccgcggga gggcggcctg gtcgcaacat
     5101 cggggccgtg gaccgtcagt gcgcgcgtgc agagcttcca gacgctgcag gatccgaacg
     5161 cgccggtgat tccaccgtac aaccgggtgc cgcagatcct cgcgtcactg cgcgataccg
     5221 actggctcgg cctcacatgg agcggcaccg gcgaatacgc gcgcttcgcg caggaggcgc
     5281 tcgtgcccac cggcgagcgg atcgtcgcgt atccgaccgt ggcgtggatg cggcagggct
     5341 cggcctggtt cttcaatgcg cgcggcggcg tgcacatgcg cgaatacctg ctcgaccaga
     5401 cgacgccggc acttcccgat cgccatccga gctacgcgat tccgatcgcg agcatcgaca
     5461 cgggtctcgt gttcgagcgc gacctcgacg tcgtcaagac gccgctgacg cagacgctcg
     5521 agccgcgcgc cttctacgtc tacatcccgc accggcagca gacgaacgcg ccggtgttcg
     5581 acacggcgct cgacgatttc aacttttcgc agctcttcgc cgagaaccgc tacatcggca
     5641 acgatcgcat cggcgacgcc aaccagctca cgctggcact gacgtcgcgc ttcctcgatc
     5701 gcgacaccgg ggcggagcgc ctgcgtctgg ccgttggcca gcgcttctat ttcgaggacc
     5761 agaaggtcag cctgaacgag ccgctgcgct cggcgtcgac gtccgatttc ctcgtgtcgg
     5821 ccgagggcag gctgtcgaac gcctggtcgc tcacgagcct tctgcaatac aacttcgacg
     5881 cgcacgaggt cgagcggctc aatgcaggcg tgcgctatac gccgacggcc ggcagcgtgc
     5941 tgaacgcgac gtggcgctat acgcgcacga tcgtcgatcc gaccggcgcg atcggcgaga
     6001 tcaagcaggt cgacctgtcc ggccagtggc cggtgggcgg gcgctggaat ctgatcgggc
     6061 gctggaatta ctcgttcacc gaccgcaaga cgctggaggc ggtggcgggc atcgagtaca
     6121 atgccgactg ctgggtgctg cgcgtcgtcg gccagcggct caccaccacg acgcagcagg
     6181 cgacgacgtc ggtgttcgtg cagctcgagc tcaacgggct cgcccgcgtc ggaacgagtc
     6241 cgctcgagct cctgcggcgc agcgtaccgg gctacgtgcc caccaacgac ccgtcgctgc
     6301 gcgagcgcga ccgtcgcctc gaagcgctcc ccgaattctg aaaggcaatc caccaatggc
     6361 cgctcgccat tcatatgacc acgcactccg ccggccttcc gcgctgcagt gggcgctgct
     6421 gctgctcgtg ctcttcgcgg ctttcgcgtt catgcagccg ctcgcgcacg cggcggcgaa
     6481 tccggtgccg ctcgaccgcg tgctcgtcat cgtcaacgac gaggcgatca cgcagtggga
     6541 catgagcgag cagcgacgca tcctgctgtc gcagatgcag gcgtcgaaga tcacgccgcc
     6601 gcccaacgac gtgctcgaca agcaggtgct cgagcggctg atcgtcgagc gcgcggtgct
     6661 gcaatacgcg aaggagaccg gcattcgcat cgacgacacc accgtcgagc gcacgatcct
     6721 gcgcgtcgcc gaggagaaca agctctcgcc cgacgagttc cgcaaggtcc tcgagcgcga
     6781 gggcattccg tacgccaact atcgcgagga catccgccgc cagatcacga tccagcgcgt
     6841 gcgcgatcgc gaggtcgaca gcaaggtcac cgtgtcggat ggcgaggtcg acaactacct
     6901 cgccacggtt gcatcgcagg cgggcggcga ggatgaatac ctgctgtcgc acatctacat
     6961 cagcgtggcc gagcaggcgt cgccaaccgc ggtggacgag agccgcaagc gtgccgagca
     7021 ggcgcttgcc gaagtgcgct cggggaagga tttcgggcag gtcgccgcgt cgtattcgag
     7081 cgcgcccgac gcgacgtcgg gaggcaacct cggctggcgc acgcgcgcac ggctgccgtc
     7141 ggtgttcgcc gatatcgtgc agaagatgaa gcccggcgaa gtgtcggccg tgctgcgcag
     7201 cgcgagcgga ttccacgtgg tgaaactcgt cgaggagcgc aaccgcaacc agcctaccgt
     7261 cgtcgaccag acgcatgcgc gccacatcct gatcaaggtc aacgagcaga cgtccgaatc
     7321 ggatgcgaag atcaagatcg accgcttgcg cgagcggctc gtcgccggcg cgaacttcga
     7381 ggagctcgcg cgcgccaact ccgaggacgc gtcgaacgcc aaaggcggcg atctcggctg
     7441 gctctccgcg ggcgacacgg tgcccgattt cgagcatgcg atggacaggc tcgcggtgaa
     7501 cgagatctcg cagccggtgc gcacgccgtt cggctggcat ctgatccagg tgatcgagcg
     7561 gcgcaagcag gacgtcacgc aacagcggcg gcgcgacgag gcgcgcaagg cgattcgcca
     7621 gcgcaagagc gacgagcagt tcgaggagtt cgtccggcaa ttgcgcgatc gcacctacgt
     7681 cgagtacaag accgacgatc gctagcgcgc atcgcgcgcg ccgtcgtacc ggggcggtca
     7741 cgtccgccgc aggatcgcga cgcgctccaa tgtgaattgc gcgcacggcg cgacacggat
     7801 cgtcatccga agctgatcga cgtcgatcgc gcgcgtgatc cgcgggatcg cgatgacgcg
     7861 gcccgccgcc gtgcaggcga gactgctcgc gcgtgcgtcg acgagttgct gccagcgtgc
     7921 gccgtttgcc ccgcgtgcgt cgatgcgaat cgcctcgggc gccacatcgc ccggcaaccc
     7981 ggcaacgacg gcgtcgatgt cgccgcaccg cagcgccact tccggcggtg agacgagcgt
     8041 cacgcggccc cggcgatcgg ccgtcagcgc gagaccgccg ggcagtgcgc gcatcgatcc
     8101 cgcgtcgagc gtccatccgt cgtcgtccgc gtgcaccggc gtcccgaatg tccacagcct
     8161 gcttccagcc ggaagtcccg cgcgctcgag caggaagtcg caggcggcct cctcgagcgg
     8221 cgtgttgcgc cacgcggtga acgtcacgta ctcggcatcg tgcgcatgcg cgccgttcga
     8281 gccgttccac gccatcggcg agacgaagcg cgcaccctgg ttccacaacg cgcgcagcgc
     8341 gcgatacgcg ctcgcgtacg tgggttgccg ctcgggatgg cgaaagtcgg cggtgttgaa
     8401 ttcgacgatg ccgaaatccg cgtcgaccga cgcaagggtg gcgtagagcg agcggccgtt
     8461 ttccatcgga acgtcattgg tcgcggccgc gccgtagacg atcgcgccga gatggccgtc
     8521 gcgcggcttg ctgccttcga tgcacacgcc gcccgagtcg aagttgcgcg gcgcactcga
     8581 ttcgcgcagc gcgagcggca tgaagccttc cggcggcgca tgcaggtccg acggcgcatc
     8641 gagtccctgc gacgaccaga tgcagccgcg gggaatgccc gcatcgcata cccagcgcgc
     8701 gagctcgtcg taatgaaggc gcacgagatg gcgccggaac acttcccact cggtcaccca
     8761 cgggtccttc cagtgcggat gatcggtccc gtgcacgaac ctgcgcggcg ggtcgaccgc
     8821 ttcgaaactc gcgaaacgcc gttgcgcaac cgcgcacacc tcgtcgagcg tcagcgggtg
     8881 ccgccgccgg tagctcgaaa gatcgggaac gtctgcttcg caggcgccgg catacggtcc
     8941 cgagcccgcg agccaatgcc ggaattggcg cagcgtgccg gggttgtagt cgtaccactg
     9001 cgacccgctg aagaacgggt tgatgtaaac gtcgggatcg agattgacgc cgacgacgag
     9061 atcagggtgc gcgcgcatga atccggcgag atgggccgcc gccgcctgca ggttgcgccg
     9121 cttgtaatgg cgcaccgcac gcgcgtacac gttgagcgtg agcgcgcgcg cgagctcggg
     9181 cgccttctgc gatcccggca gatgcgacag gtggtcgtcg ggcatcactt cgttgcgctc
     9241 gttccactgg cagttcgcaa cgtcctcctc gagccggtcg ttcacgtccc acgccgggca
     9301 ggttccggag gcatccgccc agatgccgcc gttcaacgtc accagcaccg gcagccgatg
     9361 ctgcgccgag tgcgtcagca ccggcgcgag tgcgtcgtcg gcgactgcac gcgcgccatt
     9421 gcgccggcgc acgtcgaagt ccggatcgaa ccgatagtcg ccggccacac ccgtcgtcgt
     9481 gcgcaggtac aggatcggca cgcgcatgcc gatcttcgtc gtcggcgaca catacgccgc
     9541 gtacgcccgc tcgagctcgt tcgcgccgcc cgccgagacg cccgacgttg ccggtagaag
     9601 gaaaaacggg cgcgccgggc gtttcgcaag gaacgaccag cgttcgtgcg cggccggatt
     9661 gacgagtgcg agccggccga gcgtggtttc gctgccgtcg ccggcgatcg cgatcacgcg
     9721 taccgcgccg cgggccacga gattcggcag cgggccaagc gcacggaccg tcgcctcgaa
     9781 gccgcagtaa gcacgcgccg ggtaaccggg atagacggcg gcgacgtccg gccgcggcat
     9841 gccgtagcgc gcgacttgcg tccgatcggc gaagcggatc tcgatcgcgc gaatgccgtg
     9901 gatcgacagc gcccagcccg cgaagcggat gcgttccccg tcgccgatcg ccgccccgaa
     9961 cggtgtgtcg agccagccga tcgtgtcagg cgtgccgggt gcgggcggat tggggagact
    10021 gctgcgcttg acgatcatcg tggggcgtgg aatcgtgcaa tgctatcggg gatcgaccgc
    10081 ggcacgctcg gtcttcgcaa cacgcgatgt tccctccgct acttcaacgt ctccatcgct
    10141 ggcggcttcc gcggcgcccg ccgccgacgg cccggagcgg cgacatcccg tcggcggatg
    10201 cggcgcgcga tgcgtgcacc gttgcgaggc ttcgcgccga gccctggtac gtcgacagcg
    10261 tggcgatcga cggcgcccgc gtgtcggcgg ccggctggtc gatgcccgtc gacgatcgcg
    10321 ctccggagga gggctggttc acgatcaacg gccgctcgtt cgaccgcatc gcgtatccgc
    10381 tgccgagggc cgacgtcggt gccgtcttct ggcagcgcga aggatcggcg ctttgcgggt
    10441 tccaatgcga gatcgacgac gccggcgatc cctatccttc ggggctgatg gaaatccggc
    10501 gcgcgatcgc ttcgacgccg tgcatcgagc gcggtcgcga ttcctggttc attcccgatc
    10561 ccgcccggca tgcgaacctg cccgacgagg acaggcgctt tcgcgtgatc ggcgatcgcg
    10621 acgcacgcgg attcctcgta tcgggcgcca ccgactatca gcggatcgac cgtgtgctcg
    10681 tcgaagtggc gggcagacat ctgcacgagt tcgcgggcgt gctcgactgg ggtgtgggct
    10741 gcgggcgagt cgcgcggcac tttcccgcgg cgcatgcgcg ggcgctcacc ggctgcgaca
    10801 tcgatcgcga caatgtcgac tggtgtgccg cgaacctgcc gggaaccttc gccgcgtcgc
    10861 gcctcgtgcc gccgctgccg ttcgaggacg cgcgcttcga cgtcgtctac ggtgtgtcgg
    10921 tattcactca cctgcgcgag ccgatgcagc tcgcgtggct cgcagagctc tcgcgtgtcg
    10981 tgcgccgggg cgggctcgtg ctgacgacga cgcacggcga aacggcgatc gacttcgcgc
    11041 ggctgtcgcc gctcgattac cggcagacac gcgcgcgggt tgcgcgcgag ggcctcgtcg
    11101 tgagcgggag caatacgcag ctcgatggcc acgccgatca cgaaggcgag tacgtcaacg
    11161 tctaccacag cgccgactac gtgcagcgca cgtggagccg tcacttcgac gtgctgcaca
    11221 tcgtgcccgg ctacatcctt caccacgatc tcgtcgtgat gcgcaagcca tgaacaccga
    11281 ttcgcctgca gaccggccgc tcatcgacga ccagatcgcg tattaccgcg cacgggcacc
    11341 cgagtacgac gcgtggtggc ttcgcaccgg gcgcttcgat cgcggcgccg gcaacaacgc
    11401 cgcctggcac gcggaggtgg cgatcgtcga gggcgccgtc gccggcatgc tcgcgaaggc
    11461 gcggccgtcg agcgtactgg agctcgcctg cggcaccggg cttttcacgc ggcacctcgc
    11521 accccgcgtc ggcagcgtga cggccgtcga tgcgtcaccc gaagtgatcg ccatcaatcg
    11581 cggccgcgtc ggcgcgccga acgtccggta cgtcgaggcg gatctgttcg cgttcgagcc
    11641 gcagatgcgc tacgactgcg tgttcatgag cttctggctc tcgcacgtcc cgcacgcgcg
    11701 cttcgacgcg ttctggtcga tggttcgccg ggcgctggcg ccgggcggcg tcgcgttcat
    11761 tgccgattcg gcgcacgacc cgacgtcgca cgcggtcgat catccgacgc ccgaccgcca
    11821 ggcgggcatc gtcacgcgtc gcctcgacga cggccgcgag ttccggatcg tgaaggtgtt
    11881 ctgggagccg gccaagctcg tggcgcgcct cgccgcatgc ggcttcgcgg ccgccatcgc
    11941 gggcacgccg cgctatttca tttacggcga ggcgcgcgtg gccggcgatg ccggatagag
    12001 aagcagtcgc gcgagcctga cgccggcggc attggcggga aagctcaacg agacgcgcag
    12061 gcgcgtgacg atcgtgcgcg aattgttcca gctcgccggc catttgaacg cgagctcggg
    12121 gccgctcgtc gcgccgaccg cgcgccacgg ccccgacggc tgctcctgcg cctcgacggt
    12181 cattcgcgac ggcttcacgt tcgcatcgaa gcgcagcgcc agcgaggcga gacgctgcgg
    12241 tcgaatcacc tgatcgggcg gcgaaagcag cgtcacttcg cgcgcggccg gctgcagcgt
    12301 gacaaaaccg gcgccgggcg tgagcgtgcc gcgctcggcc gtccagccgt cggcgtcgac
    12361 gtgcaccggc gtgccgaacg tccacagccg cgcaccgccg ggcacgtcgg cgtgcgaaac
    12421 gagaaaatcg cgcatcgcct cctcggccgg cgtgttgcgc cacgacgtgt acggcacgta
    12481 cccgggctgg tcggcgtaga tcccgttcga gccattccac gccatcgccg acacctcgcg
    12541 ggcgccgttg ttgaacgcgt cgcggaacgt ttggtacgcc atcgcgtagt cgggcttttt
    12601 ttgcggaaac ttgaggtcgg tattgttgta ttcgacgatg ccccagccat cgtccatccg
    12661 cccgatcgtc gcgaacagcg aatggccgcc ctcgaggacg accgcgttgc gcgcgctgcg
    12721 gccgtacagg atcgcgccga gatggccgtc gcgggggatc gcgccttcga ccgagacgcc
    12781 cgcggaatcg tagctcgcgc tgtcgctgtc gacgcgcacg gcgaacggcg gaagctccgg
    12841 gtcgttgtgg atgagcccct gcgcgctgta gatccgttcg cgcgggatgc cggccgcgtg
    12901 cgcccagctc gaaagctcgt cgtaatgcag atcgacgatg tgcttgcgga agcggtccca
    12961 caggttccac cacggatcgt cccagatcgg ccgctcgccc ggcgcaagcg gttgcacgat
    13021 tcccggcagg cgccgcggcg gctcgacctg gtcccacgat ttccactgcc tgccggcgat
    13081 cctgttgacg tcggcgaggg tcatcgggcg cgcacgccgg taggccgaca ggtcgggcgc
    13141 atgatcggtg ggccggccgg catagggccc cgatacgcgt agccattcgc ggaactggcg
    13201 cagcattccc gggttgtaat cgaacgactg ggtcccacgg aagaacgggt tcatgtacgt
    13261 gtcggcatcg agaacgacgc cgacgaagag gtcggggtgc tcgcgcgcga atgccgcgac
    13321 gatgcgcgcg gccgcctgca ggttgcgctt cttgtagcgc cgcacgtccg atgcgtagac
    13381 gttgtacgtg agcgagcgcg cgagctcggg cgacgcggtc gacccgttca ggttcttgag
    13441 gtaatcgtcg gggaatgcct cgttgtcctg tgtccactgg acgttcgcct tgtcctgctc
    13501 gaggtgatcg gtcaggtccc actgcggcgt ttcgcagctc gcatcgctcc agatgccgcc
    13561 gttcaggatg aactgcaccg gcagccgatg cgcgagcgca tagtcgatca ccgacttcag
    13621 gttgtcctcg gccaccgggc gcgtgccgca gaggcgcttc gtgtcgaacg cggggtcgaa
    13681 gacccagtcg ccccgggtac ccctggtcgt gcgcagataa aggatcggca ccgcgacgcc
    13741 gaccttctgc gtgcgcgaca ggtaatcgcg gtattcggta tcgacttccc gcgcgcctcc
    13801 ggcggcgatg ccggacgtca tcatcaggaa atagaacggc tgtcgcgcaa gtgccggatg
    13861 cgcgtcgagc cgcgcgctcc acagggtgag cgcgtgcggc ggaatcgggc gcttcggggg
    13921 cggtgcaggc gtcgccgcgt tctgcgcggc ggcggcaagg gcgacgacga cggcgacgac
    13981 tgcggaccag cgcgacgcgg cccggcccgc gcggcgcacg cccacgctac tcgagcgcgt
    14041 aatagacgta gcccttctgg ggcacgcggc ctgcgttcat ttcgcgttgc gcctcgacgt
    14101 cggatgggag cttgtcccac cagatcctac gtccgatgcg ctgctccgtt tcgatctcgg
    14161 gctttttcgc gagcaggtcg ccgatgaagc gcgtgtgatc ggaaacgtag tggcgtgcgg
    14221 gaaggaaagg catggcgatc ggcttgtcga acgtgacagc gattttatca cctgcgcatc
    14281 gaaccctcga cgagcgggaa caggaacaga cggcattcga tcgcgccgtt ccacagcgga
    14341 atgcgccgct cgggcttgag cccgatcagc ttcgcaaggc gcaggtcgcc cgtgaaaagg
    14401 cccgcggtcc atccgctgaa gcgccgcttc aatgcattgc cgagcagcgg gtagaacgcg
    14461 gcaagctgcg cggcatcggc gaggcgaacg ccgtacggcg gattggcgag cagcagtccc
    14521 gacgacgctg gtgctgcgcg cgtgagcaca tcggccgctt cgacgtgcac gaacgcgtcg
    14581 acctgtgcat tgcgcaacgc cgatgccgtg cgtgcaatgg cggcaggcgc aatgtcgctc
    14641 gcgacgatcg gcggcgtggc aggcgcttcg cgcacgcggt cgtgtgcgcg ctgcctgatc
    14701 cgctgccacg ccggtccgtc gtaccacgac agcttctgga agccgaagct gcgcgacagt
    14761 ccgggcgccc gatcggccgc gatctgcgcc gcctcggcca cgatcgtgcc gctgccgcac
    14821 attggatcga gcaacgcgcg ctcgggcgtc cactgcgcaa gtgccagcaa tcccgcggca
    14881 agattctccc gcagcggcgc ctcgtcggcg tcacggcggt agccgcgctt gaaaaggggc
    14941 tcacccgacg tgtcgaggta gatcgacgcg tcgcattcgg tgaggtgcac gtgcacgcgc
    15001 acgtcgggcg cccgcttgtc caccgaaggg cgcacggcgc agtcgctgcg gaagcgatcg
    15061 cacacgccat ccttgacgcg aagcgtcgcg aattcgaggc tcgcgagcgg agagcgggtc
    15121 gcggcgacgt cgacgcgcag cgttcgctcg ggcttgaagt gacgcatcca gtcgatgcca
    15181 tgcacgagcg catagaggtc gcgctcgtcg cgatacgggc cgctgccgac gcgccagagg
    15241 acgcggctcg cgatccgcga ttcgaggttc gcgcgatagg cgaggtcggt ttcgcccgcg
    15301 aagccgacgc cgccctcggc aggagtgatg tcaccggcat cgagtgccgc gagctccgcg
    15361 gcgagcggcc gctcgaggcc gcgcggacag ggggcgaaga agcgctcggt catcgcgcag
    15421 tgcgatcaga acggcttcgc gacgacgagc cacacgatcg cgacgagaag cggcagagcc
    15481 ggaatctcgt tcagccagcg gtagaacgcg tgaccgtgcc ggttgccgtc gcgggcgaag
    15541 tcgcgcagca gcagtccgag atagacgtgc agcgcgatca gcgcgagcac gagcgcgacc
    15601 ttggcatgaa gccagccgcc gctcacaccg aagcccagcc acagccacag gccgaacacg
    15661 atcgtgagca cggcacccgg cgtcatgatg ccgatgtaga gcttgcgctc catcaccttg
    15721 aaccgggcga tgccgcgcgc atcgtccgcc ggcgtcatcg cgtggtagac gaacaggcgc
    15781 ggcaggtaga agagcccggc gaaccacgac accatgaaca cgatgtgcag cgtcttgacg
    15841 agagcgtaca tcgggatcgt cagcccccgg atcgcgcgcg cgtcacttca gcagccgccg
    15901 ccgcgtgagc gacagcgcgg cgtaataggc gacgagcgcg tacgctgcga gcacggcgac
    15961 gtgcagcacg atgttgtccg gcacgcgatc gagcatcagc ggccgtgcga cgtcgatcgc
    16021 gtgcttcaac ggcagtgcgg cggcaatcgt cgcgagccaa gccggcatct ggtcgaccgg
    16081 gaagtagacg ccggaaaata gcagcatcgg tgtcagcacg agcgtgaaga aataggtgaa
    16141 gaaatcgtag cctggtgcga gcgagttcat cacgaggccg aacgcgccga agcagaggcc
    16201 gaccaggaat ccgagcggca gcacccatag cgcgagccac gtatggccga agccgagggc
    16261 aacgatcacg atcaggatcg ccgccgtcga catcaccgcc ttcgtcgcgc accacatcca
    16321 ctccgcgagc atcacgtcgt cgagcgcgat cggcgcgttg atgatcgcct cccacgtccg
    16381 ctggacgtgc atccgcgaaa acgccgagta catgccctcg aagctcgatg tgaacatggc
    16441 gctctggcac accatgcccg tgccgatgaa cgcgacgtac gacatgccgt tgacctggcc
    16501 gaccatcgcg ccgatgccgt agccgagcgc cagcatgtag agcagcgggt cggcgatgtt
    16561 gccgagaacg ctcgcgatcg cgagcttgcg ccacacgagc aggttgcgcc gccatacggg
    16621 gacgaagcgc accgacagtc gcggcagccg caggatcgac gtggtgttca ttcgccgacg
    16681 cctaatcgcg gagctcgcgg ccggtcagct tgatgaacag gtcctcgagg ttcgccggcc
    16741 ggtgcaggta gcgcacgccg gcgtgcgtcg cgagatcggc gagcaggggt gccgcgtcgg
    16801 tcgcatagca gaacgcggtt tcgccggcga gctcgagacg cgtggcgagc gagcgtccgc
    16861 gcgtctcggc ccaggtcttc gccgcttcgc catagacttc caccacgtcg ggctcgacgt
    16921 gctgcgcgag cagcgcgcgc ggcgaatcgc aggcgatcat cgtgccgtgg tcgatcacgg
    16981 caagccttgt tgcgaggcgc tcggcctcgt ccatgaagtg cgtcgtcagg aggatcgtct
    17041 tgccctgctt cagcagttgc cgcagcccat cccagatcag atggcgcgcc tgcgggtcga
    17101 gcccggtggt cggctcgtcg aggatcagga ggtcgggatc gttgatcagc gcacgcgcaa
    17161 gcgtgagccg gcgcttcatg ccgcccgaca gcgtgcgaat ggacgtcgcc gccttgccgg
    17221 cgaggccggc gaagtcgagc aggcgcggaa tgcgctcctc gacttcgctt cgcggcaggc
    17281 gaaagtagcc gccgtagatc agcaggtttt cgcgcacggt gaaatcggga tcgagattgt
    17341 ccatctgcgg tacgacgccg acgcgaacgc gcgcttcgcg cgccgctttc gggacggctt
    17401 cgccgacgag ctcgatcgtg ccgccgtcgg gatcgatcag accgaggcag cagcgaagcg
    17461 ttgtcgtctt gccggctccg ttcggaccga gcagcgcaaa gcactcgccg cgacgaatgg
    17521 cgaacgacaa gccgcgcacc acttcctgcg tgccgtagcg cttgcgcagc ccgtcgacgc
    17581 gcagaatcgg cgcgtcgtgc agaaccgctt gcgtcagcac ggcttcggga agcgcggctc
    17641 cgttcatgcg ctgccttcgg tgcttttcgg cggccacagc gccgcgacca tccgggagag
    17701 ttgcggaagg cgtccgtgaa agaagtggcc gcagccggga aagacgacga tcggcagctc
    17761 ctgcggccgc gcccaggcga gcacatcggc gagcggcacg acgtcgtctt cgtcgccatg
    17821 gatgacgatc gtctcggccg cgacgccgcg cacggcaaag cgcctgaccg cgggtgccac
    17881 gagcacgagg cgctcgggca ccacgcgctc cgcgacgcgc gtctgcacgt acgagccaaa
    17941 tgaaaagccg gccagcgcga ccggaagcga atcgccgaag cgcgcgcgtg catcggcgag
    18001 caccgcgagc gcgtcgtcgg tctcgccatc gccttcgtcg aacgtgccgg ccgattggcc
    18061 gacgccgcgg aagttgaagc gaacggcgac gtagccgagg ccgaagaacg tcctggccag
    18121 cgtctgaacg accttgttgt cgagcgttcc gccctgtaac ggatgcggat gacagacgag
    18181 cgcgacgccg cgcacggcgg ga
//
LOCUS       DASSVX010000036        11631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1467837, whole genome shotgun sequence.
ACCESSION   DASSVX010000036 DASSVX010000000
VERSION     DASSVX010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467837"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VFO53_01770"
     CDS             complement(<1..925)
                     /locus_tag="VFO53_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8875030.1"
                     /translation="MSNVTRRDFLQALAASSGAVLLPGEAFAQGSPKPGGTLVMAVQP
                     EPPTLASYLSTSGPIGQVAAKVYDGLFEYDFNLKPVASLATSWSESADGKTITFKLRD
                     DVKFHDDKPLTSADVQFSIMDVLRKFHPRGINTFRALQSVETPDAHTVVFKLDTPAPY
                     MMMALSGYESPIVPKHLFAGTDIQNSKYANNPVGSGPFKFVEWQRGQYMRFDRNPDYW
                     RKGEPHLERIVARFIGDAQTRSAAMQTGEINYGAFGAIPYFDVARLTKVKGIASTTKG
                     YEMQSPIVMLDFNTRVKPFDDVRVRRAISYAI"
     gene            complement(1056..1637)
                     /gene="def"
                     /locus_tag="VFO53_01775"
     CDS             complement(1056..1637)
                     /gene="def"
                     /locus_tag="VFO53_01775"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET8875031.1"
                     /translation="MALLKIARMGNPILKNVAEPVADPTDPQIAKLAADMIETLEDVG
                     GNGLAAPQVHVSRRIVVYRIAAHQIPAGADFKPLPWTVLVNPVIEPLTDEKKPIWERC
                     LSIPGLHGIVPRYTRVRCTALALDGSAIERITRGFHAMLLQHECDHLDGVLYPMRMTD
                     LSTLAFNSELGERGFLFPRPVDEFVEDNAAATT"
     gene            complement(1771..2541)
                     /locus_tag="VFO53_01780"
     CDS             complement(1771..2541)
                     /locus_tag="VFO53_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014281775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET8875032.1"
                     /translation="MQTNRMKVKFAAGEPAFGLSVMIPSPQIVESAAGMGFDWVLIDC
                     EHGTVGPESMELMVMAAEASGVTPIVRPRTNAAEDILQAMDRGAQGVQVPHVNTAAEA
                     RAAVEAVKFHPQGRRSLAAGTRASGYGFRSSAADFVADANRQSLVCVQIEDQEALPNL
                     DDILRVDGVDVFFIGPSDLSQSMGHPGNPKAPVVAQAIGAALAKIVAAGKTPGMPAAA
                     AAVQGALTSGVRYIYTHLPAILASGAGAYFQSARPSRA"
     gene            complement(2541..3458)
                     /locus_tag="VFO53_01785"
     CDS             complement(2541..3458)
                     /locus_tag="VFO53_01785"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HET8875033.1"
                     /translation="MHEHPLTVCGFLGFGEVASRFAQRLRERGVRVLAYDVLLDRPGG
                     RDALVSRAAGPPPDLLPLAELTAQADIVLSTVTTDVALSAAKNCVGLLRAGQVYVDLN
                     ATSPSVKCEAASVIAQSGADFVEGAILSAIGVAGADAKVLLCGARAAEVTASLFKLGL
                     NVHAYGIEIGKASSFKMLRSVFSKGVEALLIECLLAGRRAGIEQALWEEIVATMDAAP
                     FADVGGNWVRTHASAHVRRYHEMLQVADVLRELRVDAPMTAATLASFERSTRLALKDA
                     FPAPPASPADVIAALDARIESFMNPTEGP"
     gene            complement(3478..5001)
                     /locus_tag="VFO53_01790"
     CDS             complement(3478..5001)
                     /locus_tag="VFO53_01790"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET8875034.1"
                     /translation="MANRGASVSGAITASSQEAADAGVRVLHAGGNAVDGAIAAALAT
                     CVADPSNAGLGGYGGFMLVKRRGQPARCIQFPLCAPSNASPDMLAREYPDSGPACSSV
                     PNVVGGLARATREFATLPWATLVQPAIDLARTGVVANAPTRRALELCRGLRFVSECFV
                     LQEERRGRETRLSFRQPALAATLQKLADAGPEWFYEGPLGRAAQQASQHAAGNSMLED
                     WLRQEETVEAVVAATLECDGLRICAAPLGLTGSACLFAMYTAASRISARLRLTEGNAI
                     AELASAMASVWQYRFAVPAGNDFSTVDIPEWVDAALAYDRESPVMPDPSHTAHLNAVD
                     GDGTLVALTFTHGHAPFGARWAIPGSGVIMNAGMHNFTASPVIKRRERWFGVSNMTPT
                     IAVDAEGSGLALGCPGARRIPSSIAMALAWHRLAGHGLQRAVSAGRVHAEDRTRVGCE
                     TTRLGPACVEALRRRFASVENETGESYYGPLTAIRCAPSGIELGLDDRLFGGFGAHT"
     gene            complement(5053..5398)
                     /gene="rnpB"
                     /locus_tag="VFO53_01795"
     ncRNA           complement(5053..5398)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFO53_01795"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5475..7931)
                     /locus_tag="VFO53_01800"
     CDS             complement(5475..7931)
                     /locus_tag="VFO53_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8875035.1"
                     /translation="MPDVPAASRPKILIVDDEPYNVDFLEQELDDLGYDTVSAFDGGQ
                     ALAQVRAQAPDLLLLDIMMPVMDGFAVLERLKSDTTTRDIPVVIISAVNDLSSIVKGI
                     QLGADDYLPKPFEPVILQARIRSGLDRKLRRDLEIEYLRQVERLTAAAESVQASAYDE
                     SAIAPVAARGDALGNLARVFRKMAKEVVAREQRLRQQLRQLQLDIEEQRSNAADTTTS
                     YVPMDRRQALARGTQLPQRMQGTALFADISGFTPLTESFARELGLQRGAEEVTRQVNR
                     VHAALIACVHRYQGSVVGFGGDAITCWFDADPGLRAVACGLAIQAAMREFADIVAPGG
                     LVISLGVKVAVAGGTARRMLVGDPRVQLLDVLGGRMFDELAVAEHHAMRGDVIVTDAV
                     ATALRDHAHIGDWRDDLRFAVVEALAAPVPTAPWPDIAPDAIGDEHASAFLPPAVRDK
                     VRAGQSAFLSELRPAAALFLRFEGIDYDDDANAERKLDRFTRWVQTVAQRHDGALLQL
                     TLGDKGSSFYLTFGAPVAHYDDATRAIRAALELQTPPAELDYIGCVAIGVAHGPVRAA
                     AYGSAAHRAYSVIGDKANLAARLMMQAPEHGTLCDEAVHAAAREHVAFDALAPVLVKG
                     KSLPVAVYRPRAERAHEPERDGVDRAALVDRLSPAEQLTLKTASVIGHVFAHATLQAV
                     YPDDGERQNVASHLDALVALEFIAPLADGGERVYAFAEPGTREAAYARMLFAQRRQLH
                     RDVAQWYEHAYAADPAPHYATLARHWRAADEPAKAIHYLEKAGEIARLNGAYEEAQRY
                     FTESLAIDRRGSVLSSDYAD"
     gene            complement(7924..8280)
                     /locus_tag="VFO53_01805"
     CDS             complement(7924..8280)
                     /locus_tag="VFO53_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8875036.1"
                     /translation="MKKILIVEDVDLNVELLTQLLEDDYALAIAKDGEEGVALATSER
                     PDLILMDMSLPLCDGYEATRRIRAAGLVMPIIGLSAHAMAGDAERAIAAGCDDYLTKP
                     LNDALLFDKLDHYLNA"
     gene            complement(8291..>11631)
                     /locus_tag="VFO53_01810"
     CDS             complement(8291..>11631)
                     /locus_tag="VFO53_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET8875037.1"
                     /translation="EGMADAMVRYGSSAVPGTRMEKSAVLVPLAVGDQARGLIALANL
                     DREHAFSDSDVRLLQTLANSMSVALENARLFDETQRLLKETEQRNAELAIINEVQAAL
                     ASELNIQGIYDAVGDKVRDLFRRTDMSIRIHDPRTDLVQYPYTYERGERIAIAPHPLD
                     DHGITAHVMRTRETLVINENMADALVRYGSTTIPGTLMARSAIYVPLVVGDQVRGLLG
                     LTNMEREKAFGASDVRLLSTLANTMSVALENARLFDETQRLFQEERQRAAELAIINSV
                     QQGLVSKLKIQAIHDLVGDKLRELFDTQGISLVSFDLSRNTRHYHYLFERGRRFELDD
                     APIAPLSRHIIATRRALVVNESFAESLAAIGIEASTVPGTEPTKCVVRVPILVGNEVR
                     GVIGLDNVDRENAFSDSDVRLLTTLASSMSVALENARLFEETTRLLQETEQRAAELTT
                     VNTIGQAIASQLDLDVLIRFVGERMRQTFHADIVYVALVDKATGMIRFPYAHGDEMTP
                     MRAGEGLTGRIIDTARPLLINAGLEDATSAIGATSVGAEAKSYLGVPILQGSEAIGAI
                     SVQSTQHEGRFTEADQHLLATIAANVGVAIQNARLFSETHEARAAAEQANKAKSAFLA
                     NMSHELRTPLNAIIGFTRIVRRKAEGALPAKQTENLDKVLTSAEHLLSLINTVLDIAK
                     IEAGHMDVTASNFNASQLIDQCITTATPLLKPGVSLVKAYRNDVPPAHSDQDKIRQIL
                     LNLLGNAAKFTHRGTITVAATTGAGLLSIAVTDTGIGIAEEAIGRIFDEFQQADTSTT
                     RQYGGTGLGLSISRSLARLLGGDITVTSKPDAGSTFTLTVPLTYGVQPTRQQAGVQPA
                     PPMRRDRPIILAIDDDPNDLEILRESLGEAGYEVMGAASGEEGIARAKQIRPEVITLD
                     VMMPTKDGWQVLFDLKADAATRDIPVIMLTIVDKKPLGFELGAADYLLKPFDTDAIVT
                     ALRRVADRNGGHAPKRMLIADDDPNVIDMVRQLVGAQYELHSAGDGIDALAAIARDRP
                     DVILLDLMMPRLDGFGVIEQLRQDRANPAIPVVVLTAKSLSAAEMEGLSTRVAKVIQK
                     QGLAGDALIREIESALARPPVIVN"
BASE COUNT         1823 a         3876 c         3927 g         2005 t
ORIGIN      
        1 cgatcgcgta cgagatcgcg cgccgcacgc ggacgtcgtc gaacggcttc acgcgcgtgt
       61 tgaagtccag catgacgatc ggcgactgca tctcgtagcc cttcgtcgtc gacgcgatgc
      121 ccttgacctt cgtgagccgc gcgacgtcga aataggggat cgcgccgaac gcgccgtagt
      181 tgatctcgcc cgtctgcatc gcggcgctgc gcgtttgcgc atcgccgatg aagcgcgcga
      241 cgatgcgttc gagatgcggc tcgcccttgc gccagtagtc gggattgcgg tcgaagcgca
      301 tgtactggcc gcgctgccat tcgacaaact tgaacggtcc cgagccgacc ggattgttcg
      361 cgtacttgct gttctggatg tcggtgccgg caaagaggtg cttgggcacg atcggcgatt
      421 catagccgga gagcgccatc atcatgtacg gcgcgggcgt gtcgagcttg aacaccaccg
      481 tgtgcgcatc gggcgtttcg accgactgca gcgcccggaa cgtattgatg ccgcgcggat
      541 ggaacttgcg cagcacatcc atgatcgaga actggacgtc ggcactcgtg agcggcttgt
      601 cgtcgtggaa cttgacgtcg tcgcgaagct tgaacgtgat cgtcttgccg tcggcgcttt
      661 cactccacga cgtggcgagg ctcgcgaccg gcttcaggtt gaagtcgtat tcgaatagcc
      721 cgtcgtagac cttggccgcg acctgcccga tcggcccgga cgtgctgagg taggacgcca
      781 gcgtgggcgg ctcgggctgg acggccatca ccagcgtgcc gcccggcttc gggctgccct
      841 gcgcgaaagc ttctccgggc agcagcacgg cgccggacga cgcggcgagg gcctgcagaa
      901 aatcacgacg ggtcacgttc gacatcgcgc tcctcctggg atgaacatcc ccccacgctc
      961 gctgcgctcg ctgccccccg ggggcggggt cagtcgcttg gggcggcccg gcgcgactga
     1021 ccggcgggcc ttgtcgtatt gttggccggg gaatgttacg tggtcgcggc ggcgttgtct
     1081 tccacgaatt cgtcgaccgg ccgcgggaac aggaagccgc gctcgccgag ttcgctgttg
     1141 aaggcgagcg tggacaaatc ggtcatccgc atcgggtaga gaacgccatc caggtgatcg
     1201 cattcatgct gcagcagcat cgcgtgaaag ccgcgcgtga tgcgctcgat ggcactgccg
     1261 tcgagcgcca gcgcggtgca gcgcacccgc gtatagcgcg gcacgatgcc gtgcagcccg
     1321 ggaatcgaca ggcagcgctc ccagatcggc ttcttctcgt cggtgagcgg ctcgatcacc
     1381 gggttcacca gcaccgtcca cggcagcggc ttgaaatcgg cgcccgccgg aatctggtgt
     1441 gccgcaatgc gatacacgac gatgcgcctg ctgacgtgca cctgcggcgc cgcaagcccg
     1501 ttgccaccga cgtcctcgag ggtctcgatc atgtcggcgg cgagcttcgc gatttgcgga
     1561 tccgtcggat cggcgaccgg ctcggcgacg tttttcagga tcgggttgcc catgcgggcg
     1621 atcttgagca gtgccatcgg gaatggaggt tgttgcggtt aattgaagac cggcaccgac
     1681 gagcggaaaa attcaacggt cacgagggat cattgtagcc agcatgacga ccaaaagcga
     1741 aagcgatgtt gcgtggccga acgtcgagag tcatgcgcgc gaaggccggg cgctttgaaa
     1801 ataggcgccg gcgccgcttg cgaggatcgc cggcagatgc gtgtagatat agcgcacgcc
     1861 gctcgtcagc gcgccctgga cggcagcggc cgcggcgggc atgcccggcg tcttccccgc
     1921 agcgacgatt ttggcgagtg ccgccccgat cgcctgcgcg acgacgggcg ccttcgggtt
     1981 gcccgggtgg cccatcgact gcgacagatc ggacgggccg atgaagaaca cgtcgacgcc
     2041 gtccacgcgc aggatgtcgt ccaggttcgg cagcgcttcc tggtcctcga tctgcacgca
     2101 gaccagtgac tgccgattcg cgtccgcgac gaaatcggcg gccgagctgc gaaagccgta
     2161 accggaggcg cgcgtgcctg ccgcaaggct ccggcgtccc tgcggatgga acttgacggc
     2221 ttcgaccgcg gcgcgcgcct cggccgcggt gttgacgtgc ggcacctgaa cgccctgcgc
     2281 gccgcgatcc atcgcctgca ggatgtcctc ggcggcgttc gtccgcggac gcacgatcgg
     2341 cgtgacaccc gacgcttcgg ccgccatcac catgagctcc atgctttcgg ggccgacggt
     2401 gccatgctcg cagtcgatca gcacccagtc gaagcccatt ccggcggccg actcgacgat
     2461 ctgcggcgaa ggaatcatca ccgaaaggcc gaacgccggc tcgccggcgg cgaatttcac
     2521 cttcatgcga ttggtctgca tcatggcccc tcggtcggat tcatgaacga ttcgatgcgt
     2581 gcatcgagcg ccgcgatcac gtcggccggg cttgccggtg gtgcgggaaa tgcgtccttc
     2641 aacgcaagcc gcgtcgatcg ctcgaacgag gccagcgtcg cagcggtcat cggcgcgtcg
     2701 acgcgcaatt cgcgcaggac atccgcgacc tgcagcattt cgtggtagcg gcggacgtgt
     2761 gcgcttgcgt gcgtgcgcac ccagtttccg ccgacgtcgg cgaacggcgc agcgtccatc
     2821 gtggcgacga tttcctccca caacgcctgc tcgatgccgg cgcggcgccc cgcgagaaga
     2881 cattcgatca gcagcgcctc gacgcctttc gaaaagacgc tgcgcagcat cttgaagctg
     2941 gaagctttgc cgatttcgat cccgtatgcg tggacgttca gccccagctt gaacagtgac
     3001 gcggtgactt cggcggcgcg tgcgccgcag agcagcacct tggcatcggc ccccgcaaca
     3061 ccaatcgcgg agaggatcgc gccctccacg aaatcggcgc ccgattgcgc gatgaccgag
     3121 gccgcttcgc actttaccga aggcgacgtc gcattgaggt cgacatagac ctggcccgcg
     3181 cgcagcagcc cgacgcaatt cttcgcggcg gacagcgcaa cgtccgtcgt caccgtcgac
     3241 aggacgatat cggcttgcgc agtcagttcc gccagcggca ggaggtcggg cggcgggccg
     3301 gcggctcgcg agacgagcgc gtcgcgtcca ccggggcggt cgagcaacac gtcgtaggcg
     3361 agcacgcgca caccgcgttc gcgcaagcgc tgcgcgaagc gtgaggcaac ttcgccgaat
     3421 ccgaggaaac cgcagaccgt caagggatgt tcgtgcattc gcgtaacggc gcaaaggtca
     3481 agtgtgcgca ccgaacccgc cgaacagccg gtcatcgagc ccgagctcga tgccgctcgg
     3541 cgcgcatcga atcgccgtca acggcccata atagctttcg cccgtttcgt tctcgacgga
     3601 cgcaaatcgg cgacgcagcg cttcgacgca ggccggccca agacgcgtgg tttcgcagcc
     3661 gacccgcgtg cgatcctccg catgcacccg tcccgcgctc acggcccgct gcaagccatg
     3721 gccggccagc cgatgccacg caagcgccat cgcgatgctg gatgggattc ggcgcgcgcc
     3781 gggacatccc agcgccaggc cgctgccctc ggcgtcgacg gcgatggtgg gcgtcatgtt
     3841 gctgacgccg aaccagcgtt cgcgccgctt gatcacgggg gaggccgtga agttgtgcat
     3901 gcccgcgttc atgatcacgc ccgatcctgg aatcgcccaa cgcgcgccga atggcgcatg
     3961 cccatgcgtg aacgtgagag cgaccagggt tccgtcgccg tcgacggcat tgagatgcgc
     4021 cgtgtgcgac ggatcgggca tgaccggtga ttcgcgatcg tacgcgagcg cggcgtccac
     4081 ccattcggga atatccacgg tggagaagtc gtttccggcg ggcactgcaa aacggtattg
     4141 ccagacggag gccatcgcgc ttgcaagctc cgcgatcgca ttaccttccg tcaagcgcaa
     4201 gcgtgcgctg atgcgcgaag ccgcggtata catcgcgaaa agacatgccg aaccggtgag
     4261 gccgagcggt gcggcgcaga tgcgcaagcc atcgcactcg agcgtcgcag cgacgacggc
     4321 ttcgaccgtc tcctcctggc gaagccaatc ctcgagcatc gagttgcccg ccgcgtgctg
     4381 cgaggcctgc tgcgcggcgc ggccgagcgg cccctcgtaa aaccattccg gaccggcatc
     4441 cgcgagcttc tgcagcgtcg ccgcgagtgc gggttgccgg aagctgagcc gcgtttcgcg
     4501 gccgcgacgc tcctcctgca ggacgaagca ttcgctgacg aagcgaagtc cgcgacagag
     4561 ctcgagtgca cgccgcgtgg gcgcattggc aacgacaccc gtgcgcgcga gatcgatcgc
     4621 gggctgcacc aacgtcgccc aggggagcgt ggcgaactcg cgcgttgcac gcgcgagccc
     4681 gcccacgacg ttgggtaccg aagagcacgc gggcccggaa tcgggatatt cacgcgcaag
     4741 catgtcgggc gacgcgttcg atggagcgca cagcggaaac tggatgcacc gcgcgggttg
     4801 accgcgtcgc ttgacgagca tgaacccgcc gtagccgccg agtcccgcat tcgaggggtc
     4861 ggcgacgcac gtggcgagcg ccgccgcgat tgcgccatcg accgcgttgc cgccagcgtg
     4921 aagcacgcgc acgccggcgt ccgcagcctc ctggctgctg gcggtgatgg cgccgctcac
     4981 gctcgcgcct cggtttgcca tcgccggtgc aagcggccca ggatccgggc ttccgcccgg
     5041 acgcgaaaag tcatgcgaag ccccccgata agccgggttc tgttacggcg tgcgcaatcc
     5101 gcacgctatg accgtcattc ctctaggccc gccgttgccg gcgggctcga gccgcctacc
     5161 cgcggactcg gcgagcagcg tcgtcgtccg cctatttggc gttgctccgg atggaggttg
     5221 ccgcgtttca cccgcttccc gaaggaagcg actcgtctct gtggccctgt tcctcgcctt
     5281 cgccgcggcc gcgaggcctt ggctgttgcg gccgggcgtt acccggcatc ctgctctgtg
     5341 gagcccggac tttcctctcc ctcgggcaac ccgaggcagc gacgatctgg agggcttcgc
     5401 gagggcgatt atacgtgggg accgacatga cggccgctgc cggttcgcgt cgccgcgcgc
     5461 agcggttacg agcgtcaatc ggcgtagtcg ctcgacagca ccgacccgcg acgatcgatc
     5521 gcaagcgact cggtgaagta ccgttgcgct tcctcgtacg cgccgttgag gcgcgcgatc
     5581 tcgccggcct tctcgagata atggatcgcc ttcgcaggct cgtccgcggc gcgccagtga
     5641 cgcgcgagcg tcgcgtagtg cggcgcgggg tcggctgcat aggcgtgctc gtaccactgg
     5701 gcgacgtcac gatggagctg tcttcgctgc gcaaacagca tccgcgcata cgcggcctcg
     5761 cgcgtgcccg gctcggcgaa cgcgtaaacc cgctcaccgc cgtccgcgag cggagcaatg
     5821 aactcgagcg cgaccagcgc atcgaggtgg ctggcgacgt tttgccgctc gccgtcatcg
     5881 ggatacacgg cctgcaacgt ggcgtgcgcg aagacgtgcc cgatcacgct tgcggtcttg
     5941 agcgtgagct gctcggcggg tgaaagccgg tcgacgagcg ccgcccggtc gacgccgtcg
     6001 cgctctggct cgtgcgcgcg ttcggcccgc gggcggtaga ccgcgacagg cagcgacttt
     6061 cccttgacga ggaccggtgc cagcgcatcg aacgccacgt gttcgcgcgc cgccgcgtgc
     6121 acggcctcgt cgcacagcgt gccgtgttcg ggcgcttgca tcatgagtcg cgccgcaaga
     6181 ttcgccttgt cgccgatcac gctgtacgcc cgatgtgcgg cgctgccgta agcggccgcg
     6241 cgtaccgggc catgcgcgac gccgatcgcc acgcagccga tgtaatcgag ctccgcgggc
     6301 ggcgtctgca actcgagggc ggcgcgaatc gcgcgcgtcg catcgtcgta gtgcgcaacc
     6361 ggcgcgccga acgtcaggta gaagctcgac cccttgtcgc cgagcgtcag ttgcagcagg
     6421 gcaccgtcgt gccgctgcgc aacggtctgc acccaccgcg tgaatcggtc gagcttgcgc
     6481 tcggcgttcg cgtcgtcgtc gtagtcgatg ccttcgaagc gcaggaacag cgccgccgcg
     6541 ggacgcagct cggacaaaaa tgcgctctgg ccggcgcgca ccttgtcgcg gacggcaggc
     6601 ggcaggaacg cactcgcgtg ctcgtcgccg atcgcgtccg gcgcgatatc gggccacggc
     6661 gccgtgggca ccggcgcagc cagcgcttcc accaccgcga atcgcagatc gtcacgccag
     6721 tcgccaatgt gcgcgtggtc gcgcagcgcg gtcgcgaccg cgtccgtgac gatcacgtcg
     6781 cctcgcattg cgtggtgctc ggccaccgcg agctcgtcga acatgcgacc gccgagcacg
     6841 tcgagcagct ggacgcgcgg atcgccgacg agcatgcgcc gcgcggtgcc gccagccacc
     6901 gcgaccttga cgccgagcga aatgacgagg ccacccggcg cgacgatatc ggcgaactcg
     6961 cgcattgccg cctggatggc aaggccgcac gcgaccgcac gcagtcccgg atcggcatcg
     7021 aaccagcagg tgatcgcatc gccgccgaaa ccgacgacgc tgccctgata gcggtgtacg
     7081 caggcgatca gcgccgcgtg cacgcgattc acctgccgcg tgacttcctc ggcaccgcgc
     7141 tgcagcccaa gctcccgcgc gaacgattcg gtcagcggcg tgaaacccga gatgtcggcg
     7201 aacagcgccg ttccctgcat ccgctgcgga agctgcgtgc cgcgcgccag cgcctgccgg
     7261 cgatccatcg gcacgtagct cgtggtcgtg tcggccgcat tgcttcgctg ctcctcgatg
     7321 tcgagttgca gttggcgcaa ttgctggcgc aaccgttgct cgcgcgcgac gacttccttc
     7381 gccatcttgc ggaatacgcg cgcgaggttg ccgagcgcat cgccgcgtgc cgcgacgggc
     7441 gcgatcgcac tctcgtcgta cgcgctcgcc tgcaccgact cggcggcggc cgtcagccgc
     7501 tccacttgtc gcagatactc gatttccagg tcgcgccgca gtttcctgtc cagtccgcta
     7561 cggatgcgcg cctggaggat gaccggctcg aacggcttcg gcaggtaatc gtcggcaccg
     7621 agctggatgc ctttgacgat gctcgaaagg tcgttgaccg ccgagatgat gaccaccggg
     7681 atgtcgcgcg tcgtcgtgtc cgatttgaga cgctccaaca ccgcgaaacc gtccatcacc
     7741 ggcatcatga tgtcgagcag caggaggtcg ggcgcctgcg cgcgcacctg cgcgagggcc
     7801 tgcccgccgt cgaacgcact gacggtgtcg tatccgaggt cgtcgagctc ctgctcgagg
     7861 aaatcgacgt tgtacggctc gtcgtcgacg atgaggattt tcggacgcga cgccgctgga
     7921 acgtcaggca ttcaggtaat ggtcgagctt gtcgaacagc agcgcatcgt tcagcggctt
     7981 cgtcaggtag tcgtcgcaac cggccgcgat cgcgcgctcc gcatcccccg ccatcgcgtg
     8041 ggcggacagg ccgatgatcg gcatgacgag accggcggcg cgaatgcgcc gcgtcgcctc
     8101 gtagccgtcg cacagcggca gcgacatgtc catcaggatc agatcggggc gttcgctggt
     8161 ggcgagcgcg acgccctcct cgccgtcctt cgcgatcgcg agcgcgtagt cgtcttcgag
     8221 gagctgggtg agcagttcga cgttgaggtc gacgtcttcg acgatgagga tcttcttcat
     8281 ccgcgccttt tcaattgacg atcactggcg ggcgggcgag ggcggactcg atctcccgga
     8341 tcagtgcgtc cccggcgaga ccctgcttct gaatcacctt cgcgacgcgc gtcgaaaggc
     8401 cttccatctc cgcggccgac aggctcttcg ccgtgagtac gacgacggga atggcgggat
     8461 tcgcccggtc ctggcgcaac tgctcgatca cgccgaaccc gtcgagacgc ggcatcatga
     8521 gatcgagcag gatgacgtcc gggcgatcgc gcgcgatcgc ggcaagggca tcgatgccgt
     8581 cgcccgccga atgcagctcg tactgcgcgc cgacgagctg gcgcaccatg tcgatgacgt
     8641 tcgggtcgtc gtcggcgatc agcatccgtt tcggcgcatg tccgccgttg cggtccgcga
     8701 cgcgccgcag cgccgtcacg atcgcatcgg tgtcgaacgg cttcagcagg taatcggccg
     8761 ccccgagctc gaaaccgagg ggcttcttgt cgacgatcgt cagcatgatc accggaatgt
     8821 cgcgcgtcgc cgcgtcggct ttgaggtcga acagcacctg ccagccatcc ttcgtgggca
     8881 tcatcacgtc gagcgtgatc acctccggac gaatctgctt cgcccgggcg atgccttcct
     8941 cgccgctcgc ggcacccatg acctcgtaac ccgcctcgcc gaggctctcg cgcaggatct
     9001 cgagatcgtt cgggtcgtcg tcgatggcga ggatgatggg ccggtcgcgg cgcatcggcg
     9061 gcgccggttg cacgcccgcc tgctggcgcg tcggctgcac gccgtacgtc aacggcaccg
     9121 tcagcgtgaa cgtcgagccg gcgtcgggct tgctcgttac cgtgatgtcg ccgccgagca
     9181 ggcgcgccag gctgcggctg atcgaaaggc cgaggccggt gccgccgtat tggcgcgtcg
     9241 tgctcgtgtc cgcctgctgg aattcgtcga agatgcgccc gatcgcctcc tcggcgatgc
     9301 cgatgccggt atcggtcact gcaatcgaca acaggccggc gccggtggtc gccgcgaccg
     9361 tgatcgtgcc ccggtgcgtg aacttggctg cgttgccgag caggttgagc aggatctgcc
     9421 tgatcttgtc ctggtcggaa tgcgcaggcg gaacgtcgtt gcgatacgcc ttgaccagcg
     9481 acacgccggg cttcagcagc ggcgtcgcgg tcgtgatgca ctggtcgatc agctggctcg
     9541 cattgaagtt cgaggcggtg acgtccatgt ggcccgcctc gatcttcgcg atgtcgagca
     9601 ccgtgttgat cagcgacagc aggtgctcgg cgctggtcag gaccttgtcg aggttttcgg
     9661 tctgcttcgc aggcagtgcg ccttcggcct tgcgccgcac gatccgggtg aagccgatga
     9721 ttgcattgag cggcgtgcgc agctcgtggc tcatgttggc aaggaacgcg ctcttcgcct
     9781 tgttcgcctg ctcggccgcc gcgcgcgcct cgtgggtttc cgagaacagc cgggcgttct
     9841 ggatcgcgac accgacattg gccgcgatgg tcgcgagcag atgctggtcg gcctcggtga
     9901 agcgcccttc gtgctgcgtg ctctgtacgc tgatcgcgcc gattgcctcg ctgccctgca
     9961 ggatcgggac gccgaggtac gacttggctt ccgcgccgac actcgtcgcg ccgatcgcgg
    10021 aggtcgcgtc ctcgagcccc gcattgatca gcagcggccg cgcagtgtcg atgatcctgc
    10081 cggtgagtcc ttcgccggca cgcatcggcg tcatttcgtc gccatgcgcg taagggaagc
    10141 ggatcatgcc cgttgccttg tcgacgagcg caacgtagac gatgtcggcg tggaacgtct
    10201 ggcgcatgcg ctcgccgacg aagcggatca acacgtcgag atcgagctgg ctcgcgatcg
    10261 cctgcccgat cgtgttgacc gtcgtcagct cggcggcgcg ctgttcggtc tcttgcagaa
    10321 ggcgtgttgt ttcctcgaag agccgcgcgt tttcgagggc gacgctcatg ctgctcgcga
    10381 gcgtcgtgag cagccgcacg tcggagtcgc tgaacgcgtt ctcgcggtcg acgttgtcga
    10441 gcccgatgac gccgcgcact tcgttgccga cgagaatggg cacgcgcacg acgcacttcg
    10501 tcggctcggt tcccggcacc gtgcttgcct cgatgccgat cgccgcaagg ctttcggcga
    10561 agctctcgtt gaccaccagc gccctgcgcg tggcgatgat gtgccgcgac agcggtgcga
    10621 tcggcgcgtc atcgagctcg aagcgccggc cgcgctcgaa caggtagtga tagtgccgcg
    10681 tgttcctgga gaggtcgaac gagacgagac tgatcccttg cgtgtcgaag agctcgcgca
    10741 gcttgtcgcc gacgaggtcg tggatcgcct ggatcttgag cttcgatacc agtccctgct
    10801 gcacgctgtt gatgatcgcg agctcggctg cgcgctggcg ctcctcctgg aacagccgct
    10861 gcgtctcgtc gaagaggcgc gcgttctcga gcgcgacgct catcgtgttg gccagcgtcg
    10921 agagcagcct tacgtcggat gccccgaacg ctttctcgcg ctccatgttg gtgagtccga
    10981 ggagcccgcg tacctgatcg ccgaccacga gcggcacgta gatggcggag cgggccatca
    11041 gcgtgccggg aatggtcgtc gagccgtacc tgacgagcgc gtccgccatg ttctcgttga
    11101 tgaccagcgt ctcgcgcgtg cgcatcacgt gcgcggtgat gccgtggtcg tcgagcgggt
    11161 gcggcgcgat ggcaatgcgc tcgccgcgct cgtacgtgta cggatactgg acgaggtcgg
    11221 tgcgcgggtc gtggatgcgg atcgacatgt cggtgcggcg gaacaggtcg cgcaccttgt
    11281 ccccgaccgc gtcgtagatc ccctggatgt tgagctcgga ggcgagcgcc gcctggacct
    11341 cgttgatgat cgcgagctcg gcgttgcgct gctcggtctc cttgaggagg cgctgcgtct
    11401 cgtcgaaaag ccgcgcgttc tcgagcgcca cgctcatgct gttggcgagc gtctgcagca
    11461 ggcgcacgtc ggagtcggag aacgcgtgct cgcgatcgag gttcgcgagc gcaatcagcc
    11521 cgcgcgcctg gtcgccgacg gccagcggga cgaggacggc cgatttctcc attcgggtgc
    11581 cgggcaccgc ggaggacccg tagcgcacca tggcatcggc catcccctcg t
//
LOCUS       DASSVX010000037         6371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_584652, whole genome shotgun sequence.
ACCESSION   DASSVX010000037 DASSVX010000000
VERSION     DASSVX010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584652"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VFO53_01815"
     CDS             <1..743
                     /locus_tag="VFO53_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HET8875038.1"
                     /translation="FTDVEARAKPQAILATNTSSIPLETIATALRVPSRLIGLHFFNP
                     VAKMMLVEVVAGRDSDPAIVARGAAFVRAIDKLPLPVKSAPGFLVNRVLAPYLMTAMR
                     CVDEGLAPEVIDEAAVAFGMPMGPIELADTVGLDICAAVGRLLDAQAQAPRALAAHVA
                     AGELGKKTGRGFYAWVNGRVQKQAPANVPTGLGRRLVDPLVAEAKSALAEGIVGDADL
                     VDAGAIFGAGFAPFRGGPMHYARSLQTA"
     gene            755..2428
                     /locus_tag="VFO53_01820"
     CDS             755..2428
                     /locus_tag="VFO53_01820"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HET8875039.1"
                     /translation="MERVWLSQYPPGVPAEIDVGEYQSVRDVFEESCRKYAKRPAFTC
                     MGRAITFADLDALSSTFGGWLQGIGCTRGTRVALMMPNILQYPICVFGTLRAGCTVVN
                     VNPLYTARELEHQLVDSGAEVIVVVENFARTLSQAIARTRIRHVIVTSIGELLAVKGR
                     IVDLVLRRVKRMVPPWTLPGAIRFTDAMKAGRKHALSRIPIGHDDVAFLQYTGGTTGV
                     AKGAMLLHRNIVANLLQARAWLRPFLDERRREVILTPLPLYHIFSLTANCLVFMSIGG
                     ENVLITNPRDIGGMVRTMRGCRFTAMTGVNTLFNALLNNARFRRLDFSAFRLTLGGGM
                     AVQEAVAKRWKEVTGVPLIEAYGLTETSPAATMNPLDLPQFNGSIGLPISSTDVVLRD
                     DAGNDVAAGEPGEICIRGPQVMAGYWQRPDETAKAMTPDGYFMTGDIGTIDERGYIRI
                     VDRKKDMILVSGFNVYPNEIEGIVAMHDGVLECAAVGIPDGKSGEAVKLYVVRKDDRL
                     TGSALLSHCRRHLAGYKCPREIEFRSDLPKSNVGKILRRELRDEAKDAA"
     gene            2445..2846
                     /locus_tag="VFO53_01825"
     CDS             2445..2846
                     /locus_tag="VFO53_01825"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET8875040.1"
                     /translation="MSESPVHLPPGMPALRVTPMPADANFHGDIFGGWIMSQVDIAGA
                     IPASRRARGRVATVAVNSFVFKEPVLIGDVVSIYATIERTGRTSITVAVEVYAQRNPD
                     EEIVVKVTEASLTYVAVGPDRRPRQLPPLAS"
     gene            3052..4467
                     /locus_tag="VFO53_01830"
     CDS             3052..4467
                     /locus_tag="VFO53_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HET8875041.1"
                     /translation="MKSGAVSRTRIAAAISGIAFTLSTGAAFGAGFALQESSGSAIGN
                     AFAGGAASAEDASTLWSNAAGLARLAAPEAALAVHFITPSFKFRDEGSQPAAFQPLGG
                     TGGDAGSLNVVPNLYVALPINRQWSVGLGVNAPFGLVTEYDDNWLGRFQAVKSDIKTI
                     NVNPAVSWRVNDAFAVGVGVDWQRLDAELTSRVNYSGALALAAGQAAARGLIPGALVP
                     VIVGLTPGLESKAKIEGDDSTWGWNAGLLWDVTPQTRIGASYRSSVKYNVNANVSFDN
                     PALPSLPPQLAPVVGGLATNVNAALANGGVRANIKLPDVANLSIFHQANERWDVMADV
                     QFTRWSVFKQLEFIRTTGEVLANTPENFDDAWRFSVGATYHWSDAWTFRGGLAWDQSP
                     VNSVDRTARLPDSDRMWIAVGAKYRFNRNLALDAGYVYIPMRNGDINQNAGSTASNGL
                     VSGHYDANVNIFSAQITYTFR"
     gene            4521..5537
                     /locus_tag="VFO53_01835"
     CDS             4521..5537
                     /locus_tag="VFO53_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HET8875042.1"
                     /translation="MPDFWRGCGYRLLVRAGDGTLAVTDDFLRSLLARPELAPVPESG
                     RAELALHDALLAAPRADVPAATLDAVEDADARENYAIWLRFRERLVAAASIEAAYVRL
                     FAGDGVDVPPLFVAQLTQILLRHILGEDADPLAVRAAEMLFRTQKIAVTEDGAVMAAD
                     ETTVELFAEGGAFGSLGELLARNRTPLRTVDLDVLDRDNAESYWERDERHDFSVSLNR
                     GAAALNALLRVMEAWIAHFLGVKVAIRVEREIDDAHWRWHVGLDAEASAILNDLYNRQ
                     PVDDARMKRLLCLFRLDFDNPADMRSAIAGRPVWLAMAMDERNRLRLKPQNLIMNLPL
                     ARQQ"
     gene            5554..6138
                     /locus_tag="VFO53_01840"
     CDS             5554..6138
                     /locus_tag="VFO53_01840"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HET8875043.1"
                     /translation="MNPERSKRRRASTLVLAACAWLAGCAQPEAPARIAPSAPVASAP
                     AAPAATGPLAPLSWLAGCWQGNVNAREFRETWLPLRGGMLIGAGQQVSGGAMQDYEFL
                     RIDARPDGVYFTQFSGDRKEISFKLSATATDGNDTIFTFANTADAFPARLVYRRGTEG
                     WLYETIEGALNGSDKKVIYPLRRVDCETGELVTK"
     gene            6148..>6371
                     /locus_tag="VFO53_01845"
     CDS             6148..>6371
                     /locus_tag="VFO53_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875044.1"
                     /translation="MADDASSGPAPPAKSAALRSFERMLASGKDSALLRYSIGNEHLK
                     ASEWDAAIDALEHAVALDATYTAAWKLYASA"
BASE COUNT         1062 a         2124 c         2135 g         1050 t
ORIGIN      
        1 tgttcaccga cgtcgaggcg cgcgcgaagc cgcaggcgat cctcgcgacg aacacgtcgt
       61 cgatcccgct cgagacgatc gcgaccgcgc tgcgcgtgcc gtcgcgcctg atcggtctgc
      121 acttcttcaa cccggtcgcg aagatgatgc tcgtcgaggt ggtcgccggc cgcgacagcg
      181 accccgcgat cgtcgcgcgc ggcgccgcgt tcgtccgcgc gatcgacaag ttgccgctgc
      241 cggtcaaaag cgccccgggg ttcctggtga atcgcgtgct tgcaccctac ctgatgaccg
      301 ccatgcgctg tgtcgacgag ggtctcgcgc ccgaggtcat cgacgaggcg gccgtcgcgt
      361 tcggcatgcc gatgggtccg atcgagctcg ccgacacggt cggcctcgat atctgcgcag
      421 ccgtcgggcg cctgctcgat gcgcaggcgc aggcgccacg tgcactcgcg gcgcacgtcg
      481 ctgcgggcga gctcggcaag aagacgggtc gtggctttta cgcctgggtc aacggtcgcg
      541 tgcagaagca ggcgccggcc aacgtgccga cgggtctcgg tcgccggctc gtcgatccgc
      601 tcgtcgccga ggcgaagtcg gcgctcgccg agggcatcgt cggtgacgcc gacctggtcg
      661 acgccggagc gatcttcggc gcaggcttcg cgccgtttcg cggcggtccg atgcattacg
      721 cgcgttcgct tcaaacagcc tagaagaccg cgccatggaa cgcgtctggc tttcgcagta
      781 tccgcccggc gtgcccgccg aaatcgacgt cggcgaatac cagtcggtgc gcgacgtgtt
      841 cgaggaaagc tgccgcaaat acgcgaagcg gcccgcgttc acctgcatgg gacgcgcgat
      901 cacgtttgcc gacctcgacg cattgtcgtc gacgttcggc ggctggctgc agggcatcgg
      961 ctgcactcgc ggcacgcgcg tggcgctgat gatgccgaac atcctgcagt atccgatctg
     1021 cgtgttcgga acgctgcgcg ccggctgcac ggtggtcaac gtcaacccgc tctatacggc
     1081 gcgcgagctc gagcaccagc tcgtcgattc gggcgccgag gtgatcgtcg tcgtcgagaa
     1141 cttcgcgcgg acgctttcgc aggcgattgc gcgcacgcgc atcaggcacg tgatcgtcac
     1201 gtcgatcggc gagcttctcg cggtcaaggg ccggatcgtc gatctcgtcc tgcgccgcgt
     1261 caagcgcatg gtcccgccgt ggacgctgcc cggcgcgatc cgcttcaccg atgcgatgaa
     1321 ggcgggccgc aaacacgcgc tttcgcgcat tccgatcggt cacgacgatg tcgcgtttct
     1381 tcagtacacg ggcggcacga ccggtgtcgc gaagggcgcg atgctgttgc accggaacat
     1441 cgttgcgaac ctgctgcagg cacgcgcgtg gctgcgcccg ttcctcgacg agcggcgccg
     1501 cgaggtgatc ctgacgccgc tgccgctcta ccacatcttt tcgctgaccg cgaactgtct
     1561 cgtcttcatg tcgatcggcg gcgagaacgt gctcatcacg aatccgcgcg acatcggcgg
     1621 catggtcagg acgatgcgcg gctgccggtt caccgcgatg acgggcgtca acacgctgtt
     1681 caacgcgctg ctcaacaacg cgcgcttccg ccggctcgat ttcagtgcgt tccgcctgac
     1741 gctcggcggc ggcatggcgg tgcaggaggc cgtggcgaag cgatggaagg aagtcaccgg
     1801 ggtgccgctc atcgaagcgt atgggctcac cgaaacgtcg ccggcggcga cgatgaatcc
     1861 gctcgacctg ccgcagttca acgggtcgat cggcttgccg atctcgtcga ccgacgtcgt
     1921 gctgcgcgac gatgccggca acgacgtcgc ggcgggcgag ccgggggaaa tctgcatcag
     1981 ggggccgcag gtgatggccg ggtactggca gcgtcccgac gagaccgcga aagcgatgac
     2041 gcccgacggc tatttcatga ccggcgacat cggcacgatc gacgagcgtg gttacatacg
     2101 catcgtcgac cgcaagaagg acatgatcct cgtgtcggga ttcaacgtgt atccgaacga
     2161 gatcgaaggc atcgtcgcga tgcacgacgg cgtgctcgag tgcgcggcgg tgggcattcc
     2221 cgacggcaag tccggcgagg cggtgaagct ttacgtcgtc agaaaggacg accggctcac
     2281 cgggtctgcg ctgctgtcgc attgccggcg ccacctcgcc ggatacaagt gcccgcgcga
     2341 gatcgagttt cgcagcgacc tgccgaagtc gaacgtcggc aagatccttc gccgcgaact
     2401 gcgcgacgaa gcgaaggatg ccgcgtagaa cgaaagagga gcgaatgagc gaatcccccg
     2461 tccacctgcc gcccggcatg cctgcgctgc gcgtaacccc gatgcccgcc gatgcgaatt
     2521 tccatggcga catcttcggc ggctggatca tgtcgcaggt cgacatcgcg ggcgccatac
     2581 ccgcatcgcg ccgtgcacgc gggcgagtcg caaccgttgc cgtcaactcg ttcgtcttca
     2641 aggagccggt gctcatcggc gacgtcgtgt cgatctacgc gacgatcgag cgcacgggcc
     2701 gcacgtcgat caccgttgcg gtcgaggtct atgcgcagcg caatcccgac gaggaaatcg
     2761 tcgtcaaagt gaccgaggcg agcctcacgt acgttgcggt cggccccgat cgacggccgc
     2821 gccaattacc gccgctcgcc agctagcgcc atcccgcaac gcgcctccgc ggtccggcgc
     2881 tgtatcggcc gcaccacatc cgtcgatgcg cgatgcatcc cggcgtccgc ggaacgcttc
     2941 ctgcatgaag tcaggatgta tagtgatccg taggtcaacg ccgagatcgg cgcaccgata
     3001 cgccggccgc gttcggggat gccgtcgaaa cgaaccaggg gagtttcgag gatgaaatcc
     3061 ggagccgtca gccgtacgcg catcgccgcc gcgatcagcg gcatcgcatt cacgctttcc
     3121 accggcgccg cgttcggtgc cggattcgca ctgcaggaga gttccggcag cgccatcgga
     3181 aatgccttcg ccggcggtgc cgcttcggcc gaggacgcga gcacgctgtg gtccaatgcc
     3241 gcgggtcttg cgcgtcttgc tgcgcccgaa gcagcgctgg cggtgcactt catcacgccg
     3301 tcgttcaagt tccgcgacga gggttcgcag ccggctgcat ttcaaccgct cggcgggacc
     3361 ggaggcgatg cgggaagtct caacgtggtc cccaatctct acgtcgccct gccgatcaac
     3421 cgccaatgga gcgtcggcct cggcgtgaac gcgcccttcg ggctcgtcac cgagtacgac
     3481 gacaactggc ttgggcgttt ccaggccgtc aagtccgaca tcaagacgat caacgtcaac
     3541 ccggcggtgt catggcgggt caacgatgca ttcgcggtgg gcgtcggcgt cgactggcag
     3601 cggcttgacg cggagcttac gagccgcgtc aactacagcg gggcactcgc gctcgccgcc
     3661 ggccaggcgg cggcgcgcgg gctcattccc ggcgcactgg tgccggtgat cgtcggtctc
     3721 acgcccggcc tcgagtcgaa ggcgaagatc gaaggcgacg acagcacgtg gggatggaac
     3781 gccggcctcc tgtgggacgt gacaccgcag acgcgcatcg gcgccagcta ccgatcctcg
     3841 gtgaagtaca acgtgaacgc gaacgtgagc ttcgacaacc cggcgctgcc ttcgctcccg
     3901 ccgcagctcg cgccggtcgt cggcgggctc gcgaccaacg tgaacgccgc gctggccaat
     3961 ggcggcgtga gggccaacat caagttgccc gatgtcgcga atctgtcgat cttccatcaa
     4021 gcgaacgagc gctgggacgt gatggccgac gtgcagttca cgcgctggtc ggtgttcaag
     4081 cagctcgagt tcatccgcac caccggcgag gtgctcgcga acacgccgga aaacttcgac
     4141 gatgcctggc gcttctcggt gggtgccacg tatcactgga gcgatgcgtg gaccttccgc
     4201 ggcggacttg catgggatca gtcgccggtg aactccgtcg accgcacggc gcgcctgccc
     4261 gattcggacc gcatgtggat cgccgtgggt gccaagtacc ggttcaaccg caacctcgcg
     4321 ctcgatgcgg gctacgtcta cataccgatg cgcaacggcg acatcaacca gaacgcgggc
     4381 tccaccgctt cgaacgggct cgtgagcggg cattacgacg cgaacgtcaa catcttctcg
     4441 gcgcagatca cctacacgtt ccggtaggca accggcaccg gcgcgtcctg gccacacgct
     4501 cgggaaaggc ggcgggaagc gtgcctgatt tctggcgtgg ctgcggctac cggctcctgg
     4561 tgcgtgccgg cgacggcacg cttgcggtca ccgatgattt cctgcggagc ctcctcgcgc
     4621 gcccggagct tgcacccgtc cccgaatcgg gacgggccga gcttgcgctg cacgacgcgc
     4681 tcctcgcagc accccgggcc gatgtgccgg ccgcgacact cgacgcggtc gaggatgccg
     4741 acgcgcgcga gaactacgcg atctggctgc gctttcgcga gcggctggtc gccgcggctt
     4801 cgatcgaagc ggcttacgtg aggctcttcg ccggggatgg cgtcgacgtg ccaccgcttt
     4861 tcgtggcaca gctcacgcag atcctgctgc gccacatcct cggcgaagac gccgatccgc
     4921 tcgccgtccg cgccgcggag atgctttttc gcacgcagaa gatcgcggtg accgaagacg
     4981 gcgcggtcat ggccgccgac gaaacgaccg tcgagctttt tgccgaaggc ggcgcattcg
     5041 gctcgctcgg cgagctgctc gcgcggaacc gcacgccgct gcgcacggtc gatctcgatg
     5101 tcctcgaccg cgacaacgcc gaatcgtact gggaacgcga cgagcgtcac gatttttcgg
     5161 tcagcctcaa tcgcggcgcc gccgcgctga acgcgctgtt gcgcgtgatg gaagcgtgga
     5221 tcgcgcattt cctcggcgtg aaagtcgcca ttcgcgtcga gcgcgagatc gacgacgcgc
     5281 actggcgctg gcacgtcgga ctcgacgccg aggcgagcgc catcctcaac gacctataca
     5341 atcgacagcc agtggacgat gcgcggatga agcggctgct gtgcctgttc cggctcgatt
     5401 tcgacaatcc ggccgacatg cgaagcgcga tcgcgggtcg tcccgtgtgg ctcgccatgg
     5461 cgatggacga gcgcaatcgg ctgcggttga agccgcagaa cctgatcatg aatctgccgc
     5521 tcgcgcggca gcaatgaacg tgctcccgat gcgatgaatc ccgaacgatc gaaacggcgc
     5581 cgcgcatcga cgctggtgct tgccgcatgt gcctggcttg cgggctgcgc gcagcccgag
     5641 gcaccggcac gaattgcgcc gtccgcaccg gtagcgtcgg cacccgccgc gcccgcagcg
     5701 acgggaccgc tcgcgccgct ttcgtggctt gccgggtgct ggcaggggaa cgtcaacgcg
     5761 cgcgagttcc gcgaaacgtg gctgccgttg cgtggcggca tgctgatcgg cgccggccag
     5821 caagtgtcgg gcggcgcgat gcaggactac gaattcctgc gcatcgacgc gcggccggac
     5881 ggcgtctatt tcacgcaatt ctcgggagac cgcaaggaga tctcgttcaa gctttcggcc
     5941 accgcgaccg acggcaacga cacgatcttc acgttcgcca ataccgccga tgcatttccc
     6001 gcgcgtctcg tgtatcgccg cggcaccgag ggctggctgt acgagacgat cgaaggcgcg
     6061 ctgaacggca gcgacaagaa ggtcatctat ccgctgcgcc gcgtggattg cgagaccggc
     6121 gagctcgtga cgaaataggg cgcgaccatg gccgacgacg cgtcttcagg gccggcgccg
     6181 ccggcgaaga gcgcggcatt gcgcagcttc gagcggatgc tcgcgtcggg caaggacagt
     6241 gcgctgctgc gctattcgat cggcaacgag cacctgaagg cgagcgagtg ggacgccgcg
     6301 atcgacgcgc tcgagcacgc cgttgcgctc gacgcgacgt acaccgcagc gtggaagctg
     6361 tatgcgagcg c
//
LOCUS       DASSVX010000038         7112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_384640, whole genome shotgun sequence.
ACCESSION   DASSVX010000038 DASSVX010000000
VERSION     DASSVX010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384640"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFO53_01850"
     CDS             complement(<1..368)
                     /locus_tag="VFO53_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875045.1"
                     /translation="MQHRYGYVAGDSVRRLVLGAAFALGLAFAMAPHMESVRAADLAI
                     GPMAQAGSPAGRTPAPAVAAQAPAAETAAPPKADAPDKADADAADDANDASSEPDKAS
                     PKGSDIGHHGIVIEKGAGKIR"
     gene            569..2776
                     /gene="gspD"
                     /locus_tag="VFO53_01855"
     CDS             569..2776
                     /gene="gspD"
                     /locus_tag="VFO53_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HET8875046.1"
                     /translation="MAVVSSLLRNPHRATAGHCAIRSSRWYLRRTVVLLAALLLGVGR
                     GHAQDSDTVTLNFVNADIDAVVKAVGEITGRNFLLDPRVKGTVNIVSAHPVAKPLVYP
                     TLLSALRMQGFAAVESDGITKIVPEAEAKTQAGPVAHGTVNASGDRIVTQVIPLRYES
                     SAQLVTVLRPLVSPNNTISAYIPNNAIIVTDYADNIKRIDRIVASLDQPPSGEPMLVR
                     VKNASALDVAALVNRVLTENASAPGAQVEAQQRVSLVADPRSNSILVRADNPARGARV
                     RQLIEQLDTPSRSGGNVFIVYLRNAEAAQVAETLRGLYGGERGTLPASPVTTTTSMPA
                     AGAPVGVATTTTSFGTSSAATSPLATNATGASGAFAAGGAMIEADTANNALIIMAPEP
                     VYNNLRAVIERLDTRRAQVFVEALIVEVSSDKAAEFGIQWQVLTGANRNRTGLQGIGG
                     TNFGARGSGQNIVDASVNLGSLGQGLNLGIINGSISIPGLGVISNLGLLVRALEEDTT
                     ANILSTPTLLTLDNEEARIIVGENVPFITGQYATTGTTATVQPFQTIERRDVGLVLRV
                     KPQITEGGTVRLGIYQEVSRVQENSTAGPILSKRALESSVVVDDQQIVVLGGLIQDTM
                     NDGTQKLPYAGDVPFFGSLFRYDNRSRVKTNLMVFLKPTIVRGTHEGQQITTERYDYL
                     RGQQIQTSPDARWFWKDPTVPELPPQGAMPGTPQGTTGGMPRPVQDGEPAHGR"
     gene            2751..4244
                     /gene="gspE"
                     /locus_tag="VFO53_01860"
     CDS             2751..4244
                     /gene="gspE"
                     /locus_tag="VFO53_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HET8875047.1"
                     /translation="MASPPTAAERATERIPYAFARTHGVLAWREEGEAIVVLLRSDAS
                     ADAIAELRRVLDRPLVTRPVEADVFAAELARAYNQAGAALAMSESLANEADLSRLMQE
                     MPASDDLLDGSAQAPVIRMINALLLQALRERASDLHFEPYEARAVVRFRIDGMLRDAI
                     EPPRALHGALVSRVKIMAGLDIAEKRLPQDGRIALKLGDRQVDVRVSTLPTGAGERVV
                     LRLLDQESARLDLATLGMSRDTLDTLDRLIREPHGILLVTGPTGSGKTTTLYAALSRL
                     PRDTTNMMTVEDPIEYALDGVAQTQVNARIDLTFARALRSILRQDPDVIMIGEIRDLE
                     TAQIAVQASLTGHLVLATLHTNDAASAITRLADMGVEPYLLASSLLGVLAQRLVRTLC
                     PVCRVRAAPSDGEGRLLADLGLPATQPVASANGCDACNHTGFTGRTGVYELLRIDDTL
                     RRLIHDGAGELALRDAALRAGMRRLRDDGARWIADGRTSLAELTRVT"
     gene            4321..5520
                     /gene="gspF"
                     /locus_tag="VFO53_01865"
     CDS             4321..5520
                     /gene="gspF"
                     /locus_tag="VFO53_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HET8875048.1"
                     /translation="MSAFRYEAVDAQGRLRQGVLDADNVRGVLERLRADGLTPTSVDA
                     AAGRDGSPSRARLPAAAIALLTQQLATLVQSGMPLDQSLGAVAEQADDASVAKLLRSL
                     RAHVMSGESLPAAMSRYPRTFSPLSRGLIAAGTETGRLPDVLARLAGYLDARLALRQK
                     FIVALIYPALVTIIAVGVIAVLVTYVVPQVVSVYQQSRQTLPLLTQALIGVSGFFRAT
                     GALWLAAAIGAVVTMVAALRREPSRARIHAMLLRLPGVGRLVASLDTARYASTLAILV
                     GSGAPLLRSLDAASNVVRMIPLVRAARNAASLVREGVSLSRALKEQKAFPPVLIHLIA
                     NGELSGALAPMLERAAQELEREAERRLAWVAALIQPTLIVAMGAIVLVLVLAVMLPIV
                     TMNQLVR"
     gene            5541..6059
                     /locus_tag="VFO53_01870"
     CDS             5541..6059
                     /locus_tag="VFO53_01870"
                     /inference="COORDINATES: protein motif:HMM:NF025268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET8875049.1"
                     /translation="MHDRKRVGPMAHDHRAGSMAIAIALLAFAVVAAAADLPARFDPS
                     RDAATDVAYAIALANAQGKHVIVDVGGEWCSWCHVLDRFIAGNADVRSLIDAKYIWVK
                     VNFSKENRNEALLARWPKVAGYPHLFVLDAGGRLLHSQDTGRLESGSGYDRRKFLAML
                     RRWAPPGTSVRI"
     gene            complement(6100..>7112)
                     /gene="queG"
                     /locus_tag="VFO53_01875"
     CDS             complement(6100..>7112)
                     /gene="queG"
                     /locus_tag="VFO53_01875"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET8875050.1"
                     /translation="AIGIAGVELSEEEARLMRWLDAGRHGEMDYMARHGARRARPAEL
                     VPGTVRVISARLNYWPAAAHDARAVLEDPALAYVARYATGRDYHKVLRGKLAALARHI
                     EGEVGPFGHRVFTDSAPVLEVALAARAGLGWRGKHTLLLSRDVGSYYFLGEIYTDLPL
                     PLTPPASAHCGTCTACITACPTGAIVASYELDARRCISYLTIELKGSIPEALRPLIGN
                     RVYGCDDCQLACPWNRHAVATTTEDFDVVRNGLDDASLTTLFAWTEDEFLRNTEGSAI
                     RRIGYERWSRNVAVALGNAAPGRGIVDALERRANDPSPLVREHVAWALARQRQATIQP
                     RA"
BASE COUNT         1071 a         2501 c         2444 g         1096 t
ORIGIN      
        1 cggatcttgc ccgcgccctt ctcgatgaca atgccgtgat ggccgatatc cgagcctttc
       61 ggcgaggcct tgtcgggctc gctggacgcg tcgttggcgt cgtccgcagc gtcggcgtcg
      121 gccttgtccg gcgcgtccgc cttcggcggg gcagcggtct cggccgcggg cgcctgcgct
      181 gcgacagcgg gcgccggcgt gcggcccgcg ggcgagccgg cttgcgccat cggtccgatg
      241 gcgagatcgg ccgcccgcac gctttccatg tgcggcgcca tcgcgaacgc aagacccagc
      301 gcgaaggccg cgccgagaac cagccggcgg acgctgtccc cggcgacgta accgtaccga
      361 tgctgcatgc atgctccctc gaacctgtcg ccgattggat gcagcgaggc gcgccggcgg
      421 attcagcgtt cgcgggggga aggcggcccg atgcttgccg agagcgccga taccccatgc
      481 tattatcgcc gccgattccg cttcgcgtcg ctctccggaa ggcgacgacg tcacgacagg
      541 gatccccgga gcccgaattc tcgcgtcgat ggccgtcgtt tcatccctcc tgcgcaaccc
      601 gcaccgcgcg accgcaggcc attgcgcaat ccgatcgtca cgctggtacc tccggcgcac
      661 cgtggtgctg ctggccgcgc tgctgctcgg cgtggggcgc ggccacgcgc aggatagcga
      721 tacggtcacc ctgaatttcg tcaacgccga catcgacgcg gtcgtgaagg cggtgggcga
      781 gatcaccggc cgcaacttcc ttctcgatcc acgcgtgaag ggcacggtca acatcgtgtc
      841 cgcgcatccg gtcgcgaagc cgctcgtcta tccgaccctg ttgtcggcgc tgcgcatgca
      901 gggcttcgcc gcggtggaaa gcgacggcat cacgaagatc gttcccgaag ccgaagcgaa
      961 gacgcaggcg gggccggtcg cgcacggcac ggtcaacgca agcggcgacc ggatcgtcac
     1021 gcaggtcatt ccgctgcgct acgagtcgtc ggcgcagctc gtcaccgtgc tgcggccgct
     1081 cgtctcgccg aacaacacga tctcggcgta catcccgaac aacgcgatca tcgtcaccga
     1141 ctacgccgac aacatcaagc gcatcgaccg catcgtcgcg tcgctcgacc agccgccgtc
     1201 gggcgagccg atgctcgtgc gcgtgaagaa cgcgtcggcg ctcgacgtgg cggcgctcgt
     1261 gaaccgcgtg ctgaccgaga acgcgtccgc gccgggtgcc caggtcgagg cgcagcagcg
     1321 tgtctcgctc gtcgccgacc cgcgctcgaa cagcatcctc gtgcgcgccg acaatcccgc
     1381 gcgcggcgcc cgcgtgcgcc agctgatcga gcagctcgat acgccgtcgc gcagcggtgg
     1441 caacgtgttc atcgtctacc tgcgcaacgc cgaggcggcg caggtcgccg aaacgctgcg
     1501 cggactttac ggcggcgagc gcgggacgct tcccgcatcg ccggtcacca cgacgaccag
     1561 catgcctgcc gcgggggcac ccgtcggcgt cgcaacgacg acgacgagct tcggcacgtc
     1621 gagtgcggcc acgagccccc tcgcgaccaa tgcgacggga gcgtcgggcg ccttcgcggc
     1681 cggcggcgcg atgatcgaag ccgacacggc gaacaatgcg ctgatcatca tggcgcccga
     1741 gccggtctac aacaacctgc gcgcggtgat cgagcgtctc gacacgcggc gggcgcaggt
     1801 gttcgtcgag gcgctgatcg tcgaggtatc gtcggacaag gccgccgagt tcggcatcca
     1861 gtggcaggtg ctcaccggtg cgaaccgcaa ccgcaccgga ctgcagggca tcggcggcac
     1921 caacttcggc gcgcgcggca gcggccagaa catcgtcgac gcgtcggtca acctgggctc
     1981 gctcggacag ggactcaacc tcggcatcat caacggctcg atcagcatcc cggggctcgg
     2041 cgtcatcagc aacctcgggc tcctcgtgcg cgctctcgag gaggacacga cggcgaacat
     2101 cctgtcgacg ccgacgctgc tcacgctcga taacgaggag gcgcgcatca tcgtcggcga
     2161 gaacgtgccg ttcatcaccg ggcagtacgc gacgacggga acgacggcga ccgtccagcc
     2221 gttccagacg atcgagcgcc gcgacgtcgg cctcgtgctg cgcgtcaagc cgcagatcac
     2281 cgagggcggc acggtgcgcc tcggcatcta ccaggaggtt tcgcgcgtgc aggagaactc
     2341 gaccgccgga ccgatcctgt ccaagcgcgc gctcgagtcg tcggtcgtcg tcgacgacca
     2401 gcagatcgtg gtgctgggcg gattgatcca ggacacgatg aacgacggca cgcagaagct
     2461 tccctacgcg ggcgacgtgc cgttcttcgg gtcgctgttc cgctacgaca accgatcgcg
     2521 cgtgaagacg aacctcatgg tcttcctgaa accgacgatc gtgcgcggca cgcacgaagg
     2581 ccagcagatc acgaccgagc ggtacgacta cctgcgcggc cagcagatcc agacgtcgcc
     2641 cgacgcgcgc tggttctgga aggacccgac ggtgcccgaa ctgccgcccc agggggcgat
     2701 gcccggaaca ccgcagggca cgacgggcgg catgccgcgg ccggttcagg atggcgagcc
     2761 cgcccacggc cgctgaacgg gccaccgaac gtatcccgta cgcgttcgcg cgtacgcacg
     2821 gggtgctcgc gtggcgcgaa gagggcgagg cgatcgtcgt gctgctgcgc agcgatgcgt
     2881 cggccgacgc gattgccgag cttcgccgcg tgctcgatcg tccgctcgtc acgcggccgg
     2941 tcgaggccga cgtgttcgcc gcggagcttg cgcgcgccta caaccaggca ggcgccgcac
     3001 tcgcgatgag cgaaagcctc gccaacgagg ccgatctttc gcgcctgatg caggaaatgc
     3061 ccgcgagcga cgatctgctc gacggcagtg cgcaggcccc ggtgatccgc atgatcaacg
     3121 cgctcctgct gcaggcgttg cgcgagcgag cgtcggatct gcatttcgag ccgtacgaag
     3181 cgcgtgccgt cgtgcgcttt cgcatcgacg gcatgctgcg cgacgcaatc gagccgccgc
     3241 gcgcgttgca cggtgcgctg gtgtcgcgcg tcaagatcat ggccgggctc gacatcgccg
     3301 agaagcgcct tccgcaggac ggtcgcatcg cgttgaagct cggcgatcgg caggtcgacg
     3361 tgcgcgtatc gacgctgccg accggtgccg gcgagcgtgt cgtgctgcgg cttctcgacc
     3421 aggaatcggc gcgactcgac cttgcaacgc tgggcatgag ccgcgacacg ctcgacacgc
     3481 tcgatcggct gatccgcgag ccgcacggga ttcttctcgt cacggggccg accggctcgg
     3541 gcaagacgac cacgctttac gccgcgctgt cgcgactgcc gcgcgacacc acgaacatga
     3601 tgaccgtcga ggacccgatc gaatacgcgc tcgacggcgt ggcgcagacg caggtgaatg
     3661 cgcggatcga cctcacgttc gcccgtgcgc tgcgctcgat cctgcgccag gatcccgacg
     3721 tgatcatgat cggcgagatc cgcgatctcg agacggcgca gatcgccgtg caggcgtcgc
     3781 tcaccggcca cctcgtgctt gcgacgctgc acacgaacga cgccgccagc gcgatcacgc
     3841 gcctcgccga catgggcgtc gaaccgtacc tgctcgcatc gagcctgctc ggcgtgctcg
     3901 cgcaaaggct cgtgcgcacg ctgtgccccg tgtgccgcgt tcgtgcagcg ccgagcgatg
     3961 gcgaaggccg gctgctcgcc gatctcggct tgccggcgac gcaaccggtc gcgagcgcca
     4021 acggctgcga cgcgtgcaac cacacgggat ttaccggccg cacgggcgtc tacgagctgc
     4081 tgcgcatcga cgacacgctg cgccggctga ttcacgacgg cgcgggcgag ctcgcgctgc
     4141 gcgacgccgc gcttcgggcc ggcatgcggc ggcttcgcga cgacggcgcg cgctggatcg
     4201 ccgacggccg cacgtcgctc gcggaactca ctcgcgtgac atgacgcggc ttgcggcctc
     4261 attgtcgaag ccggctgctt cgcagcgacc gagcgcgcca gcgtttcgca gcgtcgcgcg
     4321 atgagtgcgt tccgttacga agcggtcgac gcgcaggggc gcctgcgcca gggcgtgctg
     4381 gacgccgaca acgtgcgcgg cgtgctcgag cgtcttcgcg ccgacggact cacgccgacc
     4441 tccgtcgacg ctgccgccgg gcgcgacgga tcgccttcgc gcgcgcgcct gccggcagcg
     4501 gcgatcgcgc ttttgacgca gcagctggcc acgctcgtgc aatcgggcat gccgctcgac
     4561 caatcgctcg gtgccgtcgc cgagcaggcc gatgacgcgt cggtcgcgaa gctcctgcga
     4621 tcgctgcgcg cgcacgtgat gtccggcgaa tcgctgccag ccgcaatgag ccggtacccg
     4681 cgcacgttct cgccgctctc ccgcgggttg atcgccgccg gcaccgaaac gggacgcctg
     4741 ccggacgtgc tcgcgcggct cgccgggtat ctcgatgcaa ggctcgcgct gcgccagaag
     4801 ttcatcgtcg cgctgatcta ccccgcactg gtgacgatca tcgcggtcgg tgtcattgcc
     4861 gtgctggtca cgtacgtcgt gccgcaggtc gtgtcggtgt accagcagag ccgccagacg
     4921 ctgccgctgc tgacgcaggc gctgatcggc gtcagcggct tctttcgcgc aaccggcgcc
     4981 ttgtggcttg ccgccgcgat cggcgccgtg gtcacgatgg ttgccgcgtt gcgccgcgag
     5041 ccgtcgcgtg cgcgcatcca cgcgatgcta ttgcgactgc ccggcgtcgg gcgcctcgtg
     5101 gccagcctcg acaccgcccg ctacgcgagc acgctcgcga tcctcgtcgg cagcggtgcg
     5161 ccgcttttgc gctcgctcga cgcggcatcg aacgtcgtgc gcatgatccc gctcgtgcgc
     5221 gcggcccgca atgcggcgtc gctcgtgcgc gaaggcgtgt cgctgtcgcg cgcactgaag
     5281 gagcagaagg cgtttccacc ggttctgatc cacctgatcg ccaacggcga actgagcggc
     5341 gccctcgcac cgatgctcga gcgcgcggcg caggaactgg agcgcgaggc cgagcgacgg
     5401 ctcgcctggg tggcagcgct gatccagccg acgctgatcg tcgcgatggg cgcaatcgtg
     5461 ctcgtgctgg tgcttgcggt gatgctgccg atcgtcacga tgaatcagct cgtgcggtag
     5521 cgtcgaggat cgagggagcc atgcacgatc ggaaacgcgt tggcccgatg gcgcacgatc
     5581 accgggcggg ttcgatggcg atcgccatcg cgcttcttgc gtttgccgtg gttgccgcgg
     5641 ccgccgatct gcccgcgcga ttcgacccgt cgcgcgacgc tgccaccgac gttgcctacg
     5701 ccatcgcgct cgcgaatgcg cagggcaagc acgtcatcgt cgacgtgggc ggcgaatggt
     5761 gctcgtggtg ccatgttctc gaccgcttca tcgccggcaa cgccgacgtt cgctcgctga
     5821 tcgatgcgaa gtacatctgg gtcaaggtga atttctcgaa ggagaaccgc aacgaggcgc
     5881 tgctcgcgcg ctggccgaag gtggccgggt atccgcatct tttcgtgctc gacgcgggcg
     5941 ggcgtctcct ccattcgcag gacacgggcc ggctcgaatc gggaagcggc tacgaccggc
     6001 gcaagttcct cgccatgctc aggcggtggg cgccgcccgg cacaagcgtg cgtatctgac
     6061 tcgacgcggc gcgcgtgacg gtcggggtcg cgccctgcgt catgcccgcg gttggatcgt
     6121 cgcctggcgc tgccgcgcga gcgcccacgc aacgtgctcg cgcacgagtg gcgacgggtc
     6181 gttcgcgcgc cgctcgagcg catcgacgat tccccgcccg ggcgccgcgt tgccgagggc
     6241 caccgccacg ttgcgcgacc agcgctcgta gccgatgcgc cggatcgcgc tgccttcggt
     6301 gttgcgcagg aattcgtcct cggtccatgc gaagagtgtc gtcagcgacg catcatcgag
     6361 gccgttccgc acgacgtcga aatcctcggt cgtcgtcgcc acggcatgcc gattccacgg
     6421 acacgcgagc tggcaatcgt cgcagccgta gacgcgattg ccgatcagcg gccgcagtgc
     6481 ttcgggaatg ctgcccttga gctcgatcgt gaggtacgag atgcatcgcc ttgcatcgag
     6541 ctcgtacgac gccacgatcg cgccggtcgg gcacgccgtg atgcacgccg tgcacgtgcc
     6601 gcaatgcgcg ctcgccggcg gcgtgagcgg cagcggcagg tcggtataga tctcgcccag
     6661 gaagtagtac gacccgacgt cgcgcgacag gagcagcgtg tgcttgccgc gccagccgag
     6721 gcccgcgcgt gcggccaggg cgacctccag gacgggcgcg ctgtcggtga agacgcgatg
     6781 gccgaacgga cccacttcgc cttcgatgtg ccgtgccagc gcggcgagct tgccgcgcag
     6841 gaccttgtgg tagtcgcggc ccgtggcgta gcgcgcgacg taggcgagcg ccggatcctc
     6901 gagcaccgcg cgcgcgtcgt gcgccgcggc cggccagtag ttgagccgcg cgctgatcac
     6961 gcgcacggtc cccggcacga gctcggccgg gcgcgcgcgt cgtgcgccat ggcgggccat
     7021 ataatccatc tcgccatgcc gtcccgcgtc gagccagcgc atgaggcgcg cttcttcctc
     7081 ggacagctcg acgcccgcga tgccgatcgc gt
//
LOCUS       DASSVX010000039         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_767933, whole genome shotgun sequence.
ACCESSION   DASSVX010000039 DASSVX010000000
VERSION     DASSVX010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767933"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFO53_01880"
     CDS             complement(<1..206)
                     /locus_tag="VFO53_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8875051.1"
                     /translation="MWVVDDDRSIRWVLEKALAREAIAHRTFASAYEVRQALAISQPQ
                     VLICDIRMPGESGLALLAEVKERHP"
     gene            complement(305..1399)
                     /gene="glnL"
                     /locus_tag="VFO53_01885"
     CDS             complement(305..1399)
                     /gene="glnL"
                     /locus_tag="VFO53_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HET8875052.1"
                     /translation="MPAPVAAPPIGVPALEWLATAVLLQDDQRHLVYANAAAENLFEL
                     SRRAFAGRTLSELFGPCAALEAAIDSACASGASYTEQEVALTPPGRSPLHLACTASRV
                     DAPGVALLLELRPIDHALRIAREERLAEQQQANRELIRSLAHEIKNPLGGIRGAAQLL
                     ERELDRPALAEYTQVIISEADRLRSLVNRLLGPHRAPAFRRINVHEVLVRAKGVVQAE
                     FPAIPIVCDFDSSLPELDADYEQLMQAALNIVRNAAQALAGSTTPAPAIRLVTRVARG
                     VTLVKRRHRLAVAIGVEDNGPGVPDAIRDRIFFPLVSGREGGSGLGLTIAQTLVAQHG
                     GTIECTSAPGCTVFSVLLPLPAALTNGGRA"
     gene            complement(1445..1954)
                     /locus_tag="VFO53_01890"
     CDS             complement(1445..1954)
                     /locus_tag="VFO53_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET8875053.1"
                     /translation="MTRRDPSFASRQGCIATIAGIALAAAVAAPPAHATICKYIDADG
                     NVVYSNVAPGKGLRKLSCEIADDGPQRPSGAARASTTPSTFPRVEPDTQKSRDEKRRK
                     VLDDELASEQKLLGEARDAYDNGAPPPLAGEKADAEKYRQRIARLRQTVQVHERNVEA
                     LRKEIATIR"
     gene            complement(2036..>2659)
                     /gene="glnA"
                     /locus_tag="VFO53_01895"
     CDS             complement(2036..>2659)
                     /gene="glnA"
                     /locus_tag="VFO53_01895"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417599.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET8875054.1"
                     /translation="GDNGSGMHCHQSVWKDGKNLFAGDGYSGLSDFALYYIGGIIKHA
                     RALNAITNPGTNSYKRLVPGFEAPVKLAYSARNRSASCRIPYVSSPKGRRVEVRFPDP
                     TCNPYLAFSALLMAGLDGVQNRIHPGDPMDKNLYDLAPEEAAKVPHPCASLDEALASL
                     DQDREFLTRGGVFSDDTLDAYIELKMEEVTRFRMTTHPLEYEMYYSL"
BASE COUNT          386 a          916 c          917 g          440 t
ORIGIN      
        1 ggatgccgct ccttcacttc ggcgagcagc gccaggcccg attctcccgg catgcggatg
       61 tcgcagatca gcacctgcgg ctggctgatc gcaagcgcct gccgcacttc gtaggccgag
      121 gcaaacgttc ggtgcgcgat cgcttcgcgc gcgagcgcct tttcgagcac ccagcgaatc
      181 gaccggtcgt cgtcaacgac ccacacggtg cccgcatgct cctgcgccgt cgcccgatgc
      241 agggtcgcgg cttgcggtgc gccctcggcg ctcgatcgtc cctcgatgct cgcggtttcc
      301 ccggtcacgc cctgcctccg ttggtcagcg ccgccggcaa cggcaacagc accgagaaca
      361 ccgtgcagcc gggcgcgctc gtgcactcga tcgtgccgcc atgctgcgcg acgagcgtct
      421 gcgcgatggt gagaccaagc ccgctgccgc cttcgcgtcc ggacacgagc ggaaagaaga
      481 tccgatcgcg aatcgcgtcg ggcacgcccg gtccgttgtc ctcgaccccg atcgccaccg
      541 ccaggcgatg ccgccgcttc acgagcgtca cgccgcgcgc gacacgcgtg acgaggcgga
      601 tcgccggcgc cggcgtggtg ctgcccgcca gcgcctgcgc cgcgttgcgc acgatgttga
      661 gcgccgcctg catgagctgc tcgtaatcgg cgtcgagctc cggcaggctg ctgtcgaagt
      721 cgcagacgat cggaatcgcg ggaaactcgg cctgcacgac acccttggcc cgcacgagca
      781 cctcgtgcac gttgatgcgg cgaaacgccg gtgcccggtg cggtccgagc agccgattga
      841 cgagcgagcg cagccggtcg gcctcggaaa tgatcacctg cgtgtattcg gcgagcgcgg
      901 gccggtcgag ctcgcgctcg agcagctgcg ccgcgccgcg gatgccgccg agcggattct
      961 tgatctcgtg cgcgaggctg cgaatgagct cccggttcgc ctgctgctgc tcggcgaggc
     1021 gctcctcgcg cgcgatgcgc aatgcatggt cgatcgggcg caattcgagc agcagtgcga
     1081 cacccggggc atcgacgcgc gacgcggtgc acgcgaggtg cagcgggctg cggccgggcg
     1141 gcgtgagggc cacttcctgc tcggtgtacg acgcaccgct cgcacaggcg ctgtcgatcg
     1201 ccgcctcgag tgccgcgcac gggccgaaca gctcggacag cgtgcggccg gcgaacgcgc
     1261 gccgcgaaag ctcgaacagg ttctcggcgg ccgcgttcgc gtagacgagg tggcgctggt
     1321 cgtcctgcaa cagcacggcc gtcgcgagcc attcgagtgc gggcacgccg atcggcggcg
     1381 cggcaaccgg cgccggcatg atacgaagcc gtgcagaacg gtgcagcgtc atgcgccgcc
     1441 ccattcagcg gatcgtggcg atttccttcc gcaatgcctc gacgttgcgc tcgtgcacct
     1501 gcaccgtctg gcgcagccgc gcgatccgct gccggtattt ctccgcgtcg gctttctcgc
     1561 cggccagcgg cggcggtgcg ccgttgtcat aggcgtcacg cgcctcgccg agcagcttct
     1621 gctcgcttgc aagctcgtcg tcgagcacct tgcgccgctt ctcgtcgcgg ctcttctgcg
     1681 tgtcgggctc gacgcgcgga aaggtcgacg gcgtggtcga cgcccgcgca gcgccgctcg
     1741 ggcgctgcgg accgtcgtcg gcgatctcgc aggaaagctt gcgcagcccc ttgccgggcg
     1801 cgacgttcga atagacgacg ttgccgtcgg catcgatgta cttgcagatc gtcgcgtgcg
     1861 cgggcggcgc agcgacggcc gcggccagcg cgatgccggc gatcgtcgca atgcaccctt
     1921 gccgggaagc gaatgaggga tcacgtcttg tcatccgccg gagtttatcg aagccaccgc
     1981 ggattcccaa gcgagcggcg gccttccgtg cgggcgaggc cggccgcggc gcccgtcaga
     2041 ggctgtagta catctcgtac tcgagcggat gcgtcgtcat ccgaaagcgc gtgacttcct
     2101 ccatcttgag ctcgatatag gcgtcgagcg tgtcgtcgct gaatacgcct ccgcgcgtga
     2161 ggaactcgcg atcctggtcg aggctcgcaa gcgcctcgtc gagcgacgcg cacggatgcg
     2221 ggacctttgc cgcttcctcg ggggcgaggt cgtacaggtt cttgtccatc ggatcgcccg
     2281 ggtggatcct gttctggacg ccgtcgagac cggccatcag cagcgccgaa aatgcaagat
     2341 acggattgca cgtcggatcg gggaagcgca cctcgacgcg gcggcccttc gggctgctca
     2401 cgtacggaat ccggcaggac gccgagcggt tgcgcgccga gtacgcgagc ttgaccggcg
     2461 cctcgaagcc cggcaccagc cgcttgtacg agttcgtgcc gggattggtg atcgcgttga
     2521 gcgcgcgcgc atgcttgatg atgccgccga tgtagtagag cgcgaagtcg gagagtcccg
     2581 agtatccgtc gcccgcgaac aggttcttgc cgtccttcca caccgattgg tggcagtgca
     2641 tgccggagcc gttgtcgcc
//
LOCUS       DASSVX010000040         5663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_168096, whole genome shotgun sequence.
ACCESSION   DASSVX010000040 DASSVX010000000
VERSION     DASSVX010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168096"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2281)
                     /locus_tag="VFO53_01900"
     CDS             complement(<1..2281)
                     /locus_tag="VFO53_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HET8875055.1"
                     /translation="MRIASPSPSLTANPPRELIVGEPGGVAAGSADPRVTQFKVIRRN
                     GAVVGFEPAKITIAMTKAFLAVNGGQGAGSARIRDEVTRLTDAVVAALLKRKPDGGAI
                     HIEEIQDQVELALMRGGEHEVARAYVLYREKRAQERAQQKQQQTAKSEDTTLHVVENG
                     VRKPLDFERLSDLVKVSCAGLPDAEPNRVLKATLKDLYDGVPMEEVRKCVVLAARTLI
                     EKDPAYSYVTARLLLDALRFEALGAEATHAEMETKYAEYFPQFVKHGIRIGLLNETLA
                     QFDLARLGAALKPERDLQFGYLGLQTLYDRYFLVDQYVGGRRFELPQCFFMRVAMGLA
                     LSEVDREARAIEFYDVLSTFDFMSSTPTLFNSGTHRSQLSSCYLTTVSDDLDGIYEAI
                     KENALLSKFAGGLGNDWTNVRAMGSHIKGTNGKSQGVVPFLKVVNDTAVAVNQCFAPD
                     TQVYTARGVLPIREVKPGDLVLGQSGVYREVTEKLVYEQHDAMLAIDVKHSLTPIEVT
                     DGHPFWALTGVPLEQSCERTYERIADGKLAPQWTDAGKLEVGDYVGLTMPTEIVPVEG
                     LTEDDARLYGILLGDGHLAKDGQQWGVSGNPARDEHLAFVRDYLDARGIHYWQTARGD
                     TYAQVHWAAGRGVVRDATTGRIVGAGPHTLPFAYDDLYDATHRKRIAPRLAHLPRPHT
                     LALLRGLLETDGGVSRGSEIYFTTTSQPLAEGVRYQCLRLGVPVSGQRRERDNAHVGT
                     RSDGSSIAFKGTTRTFDLRI"
     gene            complement(2714..3430)
                     /gene="pdeM"
                     /locus_tag="VFO53_01905"
     CDS             complement(2714..3430)
                     /gene="pdeM"
                     /locus_tag="VFO53_01905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HET8875056.1"
                     /translation="MSAASLSLPLDAAPRPPGGQRSLATVAGGECVVLHAGRALEWPR
                     ARTLFVADVHLGKAAAFRAGGIPLPRGSTASDLRRLDQLIAGTNAARLVVLGDFLHAR
                     AGRVAVLTQMFIAWRARHAGVDVVVVRGNHDTHAGDPPREWQVDCVDEPHALPPFLAC
                     HHLQKPRTGYALCGHVHPAVRIASAGESARLPCFVIGRERAILPAYGRFTGMADVTPA
                     HHDRIVAIAGEALFELPART"
     gene            3526..4344
                     /gene="otnI"
                     /locus_tag="VFO53_01910"
     CDS             3526..4344
                     /gene="otnI"
                     /locus_tag="VFO53_01910"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HET8875057.1"
                     /translation="MPRFAANLSFLFDEVPFLERFGEAAHAGFRAVEFAFAYEYRIQE
                     LVAEQRTHRLDVVLLNAPPGDWEQGDRGLASVPGREHEFAAGVSNALGYAKALSCSRV
                     HVMAGVVPADADDAERERRMRTYVRNLRSAATEAAAQGVTILIEPINPRDMPGYLLTT
                     QAQAHAVRSEVGARNLKVQMDFYHAQIVEGDLTEKLRRFLPHIGHVQIAGVPGRHEPD
                     GGEVNYGYIFKMLDELKYDGWVGCEYRPAQGTASGLTWLYRLLDRRPAAGKAPG"
     gene            4363..4713
                     /locus_tag="VFO53_01915"
     CDS             4363..4713
                     /locus_tag="VFO53_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HET8875058.1"
                     /translation="MALTFTWLASDFATAPQLSPEDVPQAGTAGFRSIVNNRPDFEGG
                     PSQPTSQAIRAAADAAGLDYAFLPVQSGFQTPDEIARMRELVDTLPKPVLAFCRSGAR
                     TANLYRAAKAGPGD"
     gene            complement(4710..5501)
                     /locus_tag="VFO53_01920"
     CDS             complement(4710..5501)
                     /locus_tag="VFO53_01920"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET8875059.1"
                     /translation="MQDGKGERLTATWFDLSGCVALVTGGCRGLGFAIARGLAAAGVN
                     VVVNGRKAEALAEAAARLASDGHRVTTARFDVTDAEAVRAGVAEIAAREGPVDILVNN
                     AGIQRRHALVDFPQSDWDDVIATNLTAPYLVSQAVMPAMMARRRGKIIHIASLLSEFA
                     RPSVVPYTAAKGGLRQLTRGMAVELASHGIQVNAIAPGYFATEMNRALLDDAKFDAWV
                     KARTPAGRWGAPDEIAGLAVFLASPAANYITGQMIFIDGGMSVAL"
BASE COUNT          904 a         1915 c         1913 g          931 t
ORIGIN      
        1 gaatgcgcag gtcgaatgtc ctcgtcgtac ccttgaatgc gatcgacgag ccgtcggaac
       61 gcgtgccgac atgggcgttg tcacgctcgc gccgctgccc gctcaccggc acgccgaggc
      121 gaaggcactg ataacgcacc ccttcggcga gcggctgcga cgtcgtcgtg aagtagattt
      181 cgctgccgcg cgagacgccg ccgtcggtct cgagcagccc gcgcagcagc gcgagcgtgt
      241 gcgggcgcgg cagatgcgcg aggcgtggcg cgatgcgctt gcgatgcgtg gcatcgtaca
      301 ggtcgtcgta ggcgaacggc agcgtgtggg gtccggcgcc gacgatgcga ccggtcgttg
      361 cgtcgcgcac cacgccgcgg cccgcggccc aatgcacctg tgcatacgtg tcaccgcgcg
      421 ccgtttgcca gtagtgaatc ccgcgtgcgt cgaggtagtc gcgcacgaac gcgaggtgct
      481 cgtcacgcgc cgggttgccg gagacacccc attgctggcc gtccttcgcg aggtggccgt
      541 cgccgagcag gatgccgtag agccgggcat cgtcctccgt gagtccttcc accgggacga
      601 tttccgtcgg catggtgaga ccgacgtagt cgccgacctc gagcttgcct gcgtcggtcc
      661 attgcggcgc gagcttgccg tccgcaatcc gctcgtacgt gcgctcgcac gactgctcga
      721 ggggcacgcc ggtgagcgcc cagaatggat ggccgtcggt cacttcgatc ggcgtcagcg
      781 agtgcttgac gtcgatcgcg agcatcgcgt cgtgctgttc gtagacgagc ttctccgtca
      841 cttcgcgata aacgccgctc tgcccgagca cgaggtcgcc gggtttcacc tcgcgaatgg
      901 gcagcacgcc gcgcgccgtg tagacctgcg tgtcgggtgc gaagcactgg ttcaccgcga
      961 ccgccgtgtc gttcaccacc ttcaggaacg gcaccacgcc ctgcgacttg ccgttcgtgc
     1021 ccttgatgtg cgagcccatc gcgcgcacgt tcgtccagtc gttgccgagg ccgcccgcga
     1081 acttcgacaa gagcgcgttt tccttgatcg cctcgtagat cccgtcgagg tcgtcggaga
     1141 cggtcgtcag gtagcacgac gacagttgcg agcggtgcgt gcccgagttg aagagcgtgg
     1201 gcgtcgagct catgaagtcg aacgtcgaca gcacgtcgta gaactcgatg gcgcgcgctt
     1261 cgcggtcgac ctcggaaagc gcgagcccca tcgcgacgcg catgaagaag cactgcggca
     1321 gctcgaagcg gcgtccgccg acatactggt cgacgaggaa atagcggtcg tagagcgtct
     1381 gcaagcccag atagccgaac tgcaggtcgc gctcgggctt cagcgccgcg ccgaggcgcg
     1441 cgagatcgaa ctgcgccagc gtttcgttca gaagcccgat ccggatgccg tgcttcacga
     1501 actgcggaaa gtattcggcg tacttcgttt ccatctccgc gtgcgtcgct tcggcgccga
     1561 gggcctcgaa gcgcagcgcg tcgagcagga gccgcgcggt cacgtacgag tacgcggggt
     1621 ccttctcgat cagcgtgcgt gcggcgagca cgacgcactt gcgcacttcc tccatcggca
     1681 cgccgtcgta gaggtccttg agcgtcgcct tgaggacgcg attgggctcg gcgtcgggaa
     1741 ggccggcgca cgacaccttg acgagatcgg acaaacgttc gaagtcgagc ggcttgcgca
     1801 cgccgttctc gacgacgtgc agcgtggtgt cctcgctttt cgccgtctgc tgctgcttct
     1861 gctgcgcgcg ctcctgcgcg cgcttctcgc gatagagcac gtaggcgcgg gcgacttcat
     1921 gctcgccgcc gcgcatcagc gcgagctcga cctggtcctg gatttcctcg atgtggatcg
     1981 cgccgccgtc gggcttgcgc ttcagaagcg cggcaacgac ggcgtcggtg agccgggtga
     2041 cttcgtcgcg gatgcgcgcc gagcccgcgc cctgtccgcc attcaccgcg aggaatgcct
     2101 tggtcatcgc gatcgtgatc ttcgccggct cgaaaccgac gaccgcgccg ttgcgccgga
     2161 tgaccttgaa ttgcgtaacg cgcggatcgg cgctgcctgc cgcgacgcct cccggctcgc
     2221 ccacgatcag ctcccgcggc gggtttgcgg tgagggacgg ggagggcgag gcgatgcgca
     2281 tgacggactc cttgtggttg ccttttcgtc gccgacgtcg tcggccgatg ctgttcgggg
     2341 cgcgccatcg gcgctgcttc ccgggccacg ctgcccgcga aagccggcgc cggtccgcct
     2401 gcccggcacc accggcgggc gagggtcgcc gccgggaatc gctcgccgag cacgacctac
     2461 tacatgttgt gtcatgcgag tgttatcggc accaagcata gtagccgggg gggagcagcg
     2521 caagtgtttt ttgctccaca gcagggaccg cggaaaggcg cctcgaaaca gcactctgcg
     2581 agcgcttcgc ggaacgatca cccgtgtctc caccggctat ccaccatcct gtccacaggc
     2641 tcgcgcgccg cgattgcgcg ggtccgacga acggcaacgc gagcgttgca cttttacgac
     2701 agcggctcga cgctcacgtg cgcgccggca gctcgaacag cgcctcgccg gcgattgcga
     2761 cgatgcgatc gtggtgtgca ggggtgacgt ccgccatgcc ggtgaaacgc ccgtatgccg
     2821 gaaggattgc gcgctcgcgc ccgatcacga agcagggcag tcgcgccgat tcgccggcgc
     2881 ttgcaatgcg cacggcggga tgcacgtgac cgcacaacgc gtatccggtg cgcggcttct
     2941 gaagatgatg gcaagcgagg aacggcggca gcgcatgcgg ctcgtcgacg cagtccacct
     3001 gccactcgcg cggcgggtcg cccgcgtgcg tgtcgtgatt gccacgcacg accacgacgt
     3061 cgacgcctgc gtggcgcgcg cgccatgcaa tgaacatctg cgtcagcacg gcaacgcggc
     3121 ctgccctcgc gtgcaggaaa tcgccgagca cgacaaggcg cgccgcgttc gttcctgcaa
     3181 tgagctggtc gaggcggcga agatcgctgg ccgtcgaccc gcgcggcagc ggaatgccgc
     3241 cggcgcgaaa cgccgctgcc ttgccgagat gcacgtcggc gacgaacagc gtgcgtgcac
     3301 gcggccactc gagcgcgcgc ccggcgtgca gcacgacgca ctcgccgccg gcgaccgtcg
     3361 caaggcttcg ctggccgccg ggcggccggg gcgcagcatc gagcgggagg gacagcgaag
     3421 cggcggacat cgggattgcg gaaaaacggg agcaccggtg ttcgcggcgc acaatcggag
     3481 gcacaattgt agtcgaccct caaggtacgc cgacttccgg ccgacatgcc acgattcgca
     3541 gcgaatctgt cgttcctgtt cgacgaagtg ccgtttctgg agcgcttcgg cgaggccgcg
     3601 catgccgggt ttcgcgccgt cgagttcgcc ttcgcctacg agtaccgcat ccaggagctc
     3661 gttgccgagc agcgcacgca ccggctcgac gtggtgttgc tcaacgcgcc gccaggcgac
     3721 tgggagcaag gtgaccgcgg cctcgcctcg gtgccgggtc gcgagcacga attcgcggcg
     3781 ggtgtctcga acgcgctggg ctacgcgaag gcgctgtcgt gctcgcgcgt tcacgtcatg
     3841 gccggcgtcg ttcctgccga tgccgacgac gccgagcgcg agcggcgcat gcgcacgtac
     3901 gtgcgcaatc ttcgctccgc agcaacggag gcagcggcac agggcgtgac gatcctcatc
     3961 gagccgatca atccgcgcga catgcccggt tatctgctca ccacgcaggc gcaggcgcac
     4021 gcggtgcgca gcgaggtcgg cgcgcgcaat ctcaaggtgc agatggattt ctatcacgcg
     4081 cagatcgtcg agggcgacct caccgaaaag ctgcgccgct ttcttccgca catcggtcac
     4141 gtgcagatcg ccggtgtccc cgggaggcac gagcccgatg gcggcgaagt gaactacggg
     4201 tacatcttca agatgctcga cgagctcaag tacgatggat gggtgggctg tgaataccgg
     4261 ccggcgcaag gcaccgcctc cggactcacc tggctctatc ggctcctcga ccggcggccg
     4321 gcggccggca aggcgcccgg ctgattgcaa agggaaacga acatggcact cacattcacg
     4381 tggcttgcgt ccgatttcgc gaccgcgccg cagctttcgc ctgaagacgt cccgcaggcc
     4441 ggcacggcgg gctttcgcag catcgtcaac aatcgtcccg acttcgaggg cgggccttcg
     4501 cagccgacgt cgcaggcgat acgcgcggcg gccgatgcgg cggggctcga ctacgcgttc
     4561 ctgccggtgc aatcggggtt ccagacgccc gacgaaatcg cccgcatgcg cgagctcgtc
     4621 gacacgctgc cgaaaccggt gctcgccttc tgccgctcgg gagcgcgaac ggccaacctc
     4681 taccgcgcgg caaaggcagg tcccggcgac taaagtgcaa cggacatgcc gccgtcgatg
     4741 aagatcatct gcccggtgat gtaattcgcg gcgggcgagg cgagaaacac cgccagtccc
     4801 gcgatctcgt ccggcgcacc ccagcgtccc gcgggcgtgc gggccttcac ccacgcatcg
     4861 aatttcgcgt cgtcgagcag tgcgcgattc atttccgtgg cgaagtagcc gggtgcgatc
     4921 gcgttgacct ggatgccgtg cgatgcaagc tccaccgcca tgccgcgcgt gagttgacgc
     4981 aggccgccct tcgccgcggt gtacggcacg acgctcggcc gcgcgaactc cgacagaagc
     5041 gacgcgatgt gaatgatctt cccgcgccgc cgcgccatca tcgcgggcat cactgcctgc
     5101 gaaacgaggt acggcgcggt gaggttcgtc gcgatgacgt cgtcccagtc actttgcgga
     5161 aaatcgacca gcgcatggcg gcgctggatg ccggcgttgt tgacgagaat gtcgaccgga
     5221 ccctcgcgag ccgcgatttc ggcgacgccc gcgcgcaccg cctcggcatc ggtgacgtcg
     5281 aaccgcgctg tcgtcacgcg atgaccgtcg ctcgcgagcc tcgccgccgc ttccgcgagc
     5341 gcttcggcct tgcgtccgtt gaccacgaca ttcacgccgg cggcggcgag gccgcgcgca
     5401 atggcgaagc ccaggccgcg gcagcctccg gtgacgagcg cgacgcatcc cgacagatcg
     5461 aaccacgttg cagtcaaccg ctcgcctttg ccgtcctgca tcgcattttc tcctcgtcaa
     5521 cgcgtcgcgt catgatcgtg ctcgccgcgc gccgcggcgg cttcgagatc ggccaccatt
     5581 cgcgcgacgc gcgcctcgag tgcctcggtc gtgagcttct cacgaaacag ctcgacgatc
     5641 agcgggaacg agaatggcgt cgg
//
LOCUS       DASSVX010000041        12427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_568115, whole genome shotgun sequence.
ACCESSION   DASSVX010000041 DASSVX010000000
VERSION     DASSVX010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12427
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568115"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFO53_01925"
     CDS             <1..304
                     /locus_tag="VFO53_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET8875060.1"
                     /translation="ERELPQTIRVDLDIGLPSSRPFETGELADAIDYAAVVKRLRVFA
                     ADHPHALLERFAEAIASVVLDEFGAQSVKLRVAKLGALPGVREIGVAIERNAQMRA"
     gene            360..566
                     /locus_tag="VFO53_01930"
     CDS             360..566
                     /locus_tag="VFO53_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HET8875061.1"
                     /translation="MDDETFNLSIRKFLKVVGIGSQREIERAVAQGIEEGTIKGTEGF
                     PAHMTLVVQGLNIQVSFDGTIKLQ"
     gene            568..1515
                     /locus_tag="VFO53_01935"
     CDS             568..1515
                     /locus_tag="VFO53_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HET8875062.1"
                     /translation="MKYKDYYAALGVARTATADEIKKAYRKLARKYHPDVSKEAGAEE
                     KFKEVAEAYETLKDPEKRAAYDELGRHAPGEQFRPPPGWQQSFGEQPPGASSFSFDDV
                     DLADLFAGIAGRGRGGARRPDMPLPGQDFEAAVRVSFDQAYKGTEIELDMAVPEPDEN
                     GFLRRVPRKIRVRIPKGVTDGQKLRVPGKGGKGANGARDGDLYLDIEVQSHPLYRAEA
                     LDLYMDLPLAPWEAVLGASVDVPTPAGRVTLKVPPGTHAGQKLRLSGRGLSRPDGTSG
                     HLYAVAQIVVPTVVDERQRELFTQLKDASAFNPRAHMVD"
     gene            1515..1820
                     /locus_tag="VFO53_01940"
     CDS             1515..1820
                     /locus_tag="VFO53_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HET8875063.1"
                     /translation="MKIEATTALVLDDTGTLTIAELVEQSGLSADELQVLVDCGALEP
                     RETVAPSWRFSTRCVVTARTARRLRDDLALDDTHALAIVLRFMQRIEALERELRRRG"
     gene            complement(1829..5089)
                     /gene="putA"
                     /locus_tag="VFO53_01945"
     CDS             complement(1829..5089)
                     /gene="putA"
                     /locus_tag="VFO53_01945"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HET8875064.1"
                     /translation="MHPPAQAIVSESALIPDSAAGAAIRDAFLADERTTLIALASQIA
                     QDERAERAVAQKARAWVESVRADRQSHGGIESFLQQYDLSTPEGVLLMCIAEALLRIP
                     DAATADALIRDKLSRGDWEHHLGASDSLLVNASTWGLMLTGKLTRIDSADARDPRAWY
                     ERFVARAGEPVVRVAVRQAMKVMAEQFVLGRTIEEAVANSDAQWRHSYDMLGEAAVTA
                     ADAARYFDAYRHAIATIGSYAQRRAANDGAIVGAPSISVKLSALHPRYEYPQRRRVLA
                     ELVPVVISLAQAARDAQIAMTIDAEEAERLELSLELFARVRRDPALADWHGLGLAVQA
                     YQKRARAVVGYVAGLARHTGTRIPVRLVKGAYWDTEIKRAQVQGLAGYPVFTRKPHTD
                     ICYLACAKALLDAGDAVYPMFATHNAHTIAWVSEHAARRGIHEYEFQRLFGMGEALYR
                     HVLARDRRDRGHSRNYSSPRYAQNSLPDAANNYGSDPDLPIACRVYAPVGGHRDLLPY
                     LVRRLLENGANTSFVNRIGDPRIAVDDVIADPLARARNGDFAPASNIPLPIDLFAPER
                     RNSEGVSLADQQAMRALDDALVQSASEHWTAAPGIGEAERAARQAFAPADRRACIGTV
                     TDADETDVDRALAQLVAAQPAWDARAASERAAILDRAAAGIEAQRALWVAMLAREAGK
                     TRSAAIAEVREAADFCRYYAAQARARFASPIALPSPTGESNTLALRGRGVFACISPWN
                     FPLAIFTGQIAAALAAGNAVVAKPAEQTPLIAAQVVRTLHESGVPDDVLALLPGPGET
                     IGARLVADRRVAGVAFTGSTAAGSAIAQSLAARAPIVPLIAETGGQNAMVVDSSALAE
                     QVVADIVQSAFDSAGQRCSALRLLCLQEDIAPRVLELLEGAMDELVIGDPGDLATDVG
                     PVIDEAAREALDAHVAAMDSLRLVRHRVALPGTCGNGTFVAPTLIVLDRMDRLTQEVF
                     GPVVHVTTWRASELDALVDAINGFGYGLTLGIHSRVDATIERIVSRVRVGNVYVNRNM
                     IGAVVGMQPFGGEGLSGTGPKAGGPNYLPRFAVERTLSINTAAAGGNAALLAQDDG"
     gene            complement(5104..6000)
                     /gene="xerD"
                     /locus_tag="VFO53_01950"
     CDS             complement(5104..6000)
                     /gene="xerD"
                     /locus_tag="VFO53_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET8875065.1"
                     /translation="MTNDEGMIDAFCDQLWLQDGLAASSLASYRRDLAGWCAWLAKRD
                     TALLRAARVDFERWLADQFEAKAKATSVARRLSALRRFYRFQLAQGAIAEDPTLRVRA
                     PKKPRRLPKQLSEAQVETLLAAPDTRIPLGVRDRAMLETLYATGLRVSELTGLKLSQV
                     AHDAGVVRVVGKGSKERLVPMGDEAVAWISRYLAEVRPALAGESKRDEVFLTQRHAPL
                     TRQAFWALVKRYAVRAAIPKASLSPHVLRHAFATHLLNHGADLRVVQLLLGHADITTT
                     TIYTHVARERLKQLHAAHHPRG"
     gene            complement(6004..7302)
                     /locus_tag="VFO53_01955"
     CDS             complement(6004..7302)
                     /locus_tag="VFO53_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET8875066.1"
                     /translation="MDALITTLLIVALFALLGTGVWIGLALAGVAWIGMTLFSSRPAG
                     DAMAVTIWGTASSWTLTALPLFVWMGEILFRTRLSDDMFKGLAPWLERVPGRLLHTNI
                     IGCTIFAAVSGSSAATCATIGKMTLPELARRGYPDGITIGSLAGAGTLGLLIPPSIIM
                     IVYGVSANVSIAQLFIAGIVPGILLGVLFSGYIVVWALVNPQLVPAATERTTFAQKLH
                     ASRHLIPVVLLIVLVLGSIYTGVATATEAAAFGVVGSLVLSATQGSLGWRTFRDSLLG
                     ATRLYCMIALILSGAAFLTLAMGYIGLPRDLAEWIATLGLSPFALIVALALFYIVLGC
                     FLDGISMVVLTMGVILPTVEKAGIDLLWFGIFVVLVVEMAQITPPVGFNLFVLQGMTN
                     RQITWIARHALPMFFLMCAAVLAIWFFPSLVTLLPKHMKM"
     gene            complement(7333..7842)
                     /locus_tag="VFO53_01960"
     CDS             complement(7333..7842)
                     /locus_tag="VFO53_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HET8875067.1"
                     /translation="MRAFLDWLYDAAAYVAAFFLVGTLAMVLLGIVGRLMNWFIPGTD
                     SYAGYCMAASGFLALAHTLKQGEHIRVSLLLEHAGVRARRGLELWALAVATVLAGAFA
                     FYALRLAVQSWQFSDISTGNDATPLWIPQIAMAVGTVVLFIAFVDELIGEWRGTRRTR
                     MPAEALHHE"
     gene            complement(7864..8835)
                     /locus_tag="VFO53_01965"
     CDS             complement(7864..8835)
                     /locus_tag="VFO53_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HET8875068.1"
                     /translation="MNRLAATLVLGLTLALPAFAQTKWDLPTAYPANNFHTENLQQFA
                     GDVDKATGGKLKIQLHPNASLFKAPEIKRAVQGGQAQAGEILLVNFENEDPLYGIDGV
                     PFLATSYSEALKLYKAERKALDDKLAKQGMMLLYTVPWPPQGIFTNRTLNSAADMKGL
                     KWRAYSPATSKIAELVGAQPVTVQAAEVSQALATGVIDSYMSSGATGYDSKTYEHLKH
                     FYDTQAWLPKNAVIVNRAAFNALDKTTQAAVLKAAADAETRGWKLSEEKTGWYVDQLR
                     QKGMAIDKPSEQLTADMRKVGNVMLADWLRKAGPEGKRIIDQYRATQ"
     gene            9050..>12427
                     /locus_tag="VFO53_01970"
     CDS             9050..>12427
                     /locus_tag="VFO53_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET8875069.1"
                     /translation="MQFWIDRGGTFTDIVARRADGTLVTHKLLSDNPERYRDAAVHGI
                     RELLGLAPGAPIPPGAIEAVKMGTTVATNALLERTGERTVLVITRGFADALRIGYQNR
                     PKLFVRRIELPSQLYERVIEADERIGARGEIVRPLDLETLEPALREAFADGVRAVAIV
                     LMHGYRYPQHECAIAELALAIGFPQVSISHEVSPLMKLVSRGDTTVVDAYLSPILRRY
                     VDEVERDLVGDRRDETGVVPPLRLQFMQSSGGLTDARRFQGKDAILSGPAGGIVGAVE
                     VSRLAGFERIIGFDMGGTSTDVTHWAGEYERAFVTEVAGVRLRAPMMSIHTVAAGGGS
                     ICAFDGARLRVGPQSAGANPGPASYRRGGPLTVTDCNVMVGKLDPELFPNVFGPRGDE
                     PLDAAIVRTKFEALAAQIASSTGHARTPEQVADGFLDIAIENMANAIKHISVQRGYDV
                     TGYTLCSFGGAGGQHACRVADALGMSRVFIHPLAGVLSAYGMGLADVRALKQQAVETT
                     LSPAALDATRVAFAMLEDAGRRDVEAQGIAPARIRCERTLQLKYEGTDTTLGVPTAAG
                     VDAAIGEFERRYRQQYGFLMPGKPLVIEALAVEAIGTAHDIQDERRDFAPRPGELAPF
                     RRARIHTQGDWRDAAVYAREELRPGDAIDGPAIIRERNATTVLEPGWRATLTPRDHLI
                     LERVEPIRRAHAIGTSADPVMLEVFNNLFMAIAEQMGVTLANTSYSVNIKERLDFSCA
                     LFDASGNLIANAPHMPVHLGSMGESVATIIGRRSGSMHPGDVFVLNAPYNGGTHLPDV
                     TVIAPVFLDAGAKKGPGSISMETMTEGDVRKKSTLAPFSRFAPEFFVASRGHHADIGG
                     ITPGSMPPDSTHVDEEGVLLDNVQLVADGRFLEAGMRAILLSGRYPVRNVEQNLADLR
                     AQVAACAKGAEELRKMVAHFGLPVVRAYMKHVQDNAEEAVRRVVGALSDGTFAYEMDN
                     GAVIRVAIKVDRAARSATIDFGGTSPQQPTNFNAPSAVCKAAVLYVFRTLVDDDIPMN
                     AGCLKPLSIVIPEGSMLNPRYPAAVVAGNVETSQTITDTLYGALGVLAASQGTMNNFT
                     FGNDRYQYYETISGGAGAGADFDGASVVQTHMTNSRLT"
BASE COUNT         2080 a         4148 c         4248 g         1951 t
ORIGIN      
        1 ggaacgggaa ttgccgcaga cgattcgcgt cgatctcgac atcggcctgc cgtcgtcgcg
       61 tccgttcgaa acgggcgagc tggcggacgc gatcgactac gcggcggtgg tgaagcggct
      121 gcgcgtcttc gcggccgatc atccgcatgc gctgctcgag cgcttcgccg aagcaatcgc
      181 gtcggtcgtg ctcgacgaat tcggggcgca gtcggtgaag ctgcgcgtgg cgaagctcgg
      241 cgcgcttccc ggcgtccgcg aaatcggcgt cgcgatcgag cgcaatgcgc aaatgcgcgc
      301 gtagcggcgc gcgctcgcta gaatgcacgc gtatcgcccc aaactttatg gagagcgcca
      361 tggacgacga aacattcaac ctcagcattc gcaagttcct gaaggtcgtc ggcatcggct
      421 cgcagcgcga gatcgagcgc gcggtcgcgc agggcatcga ggagggcacg atcaagggca
      481 ccgaaggctt tcccgcgcac atgacgctcg tcgtgcaggg tctcaacatc caggtttcgt
      541 tcgacggcac gatcaagctg caatagcgtg aaatacaagg attactacgc cgcgctcggc
      601 gtcgcccgca ctgccaccgc ggacgagatc aagaaggcct accgcaagct cgcgcgcaaa
      661 tatcatcccg acgtgtcgaa ggaagcgggc gccgaggaaa aattcaagga agtggccgag
      721 gcttacgaaa cgctgaagga ccccgagaag cgcgcggcat acgacgagct cggccgtcat
      781 gcgcccggcg aacaattccg gccgccgccg ggctggcagc agtcgttcgg cgagcagccg
      841 cccggtgcat cgtcattttc gttcgacgac gtcgatctcg ccgacctttt cgccggcatc
      901 gcggggcgcg gcaggggcgg cgcgcggcgc cccgacatgc cgctgccggg acaggatttc
      961 gaggccgccg tgcgcgtctc gttcgaccag gcgtacaagg gcaccgagat cgagctcgac
     1021 atggcggtgc ccgagcccga cgagaacggg tttttgcgcc gcgtgccgcg caagatccgc
     1081 gtgcgcatcc ccaagggggt gaccgacggc cagaagctgc gcgtgcccgg caagggcggc
     1141 aagggtgcca atggcgcgcg cgacggcgac ctctatctcg acatcgaggt gcagtcgcat
     1201 ccgctctacc gtgccgaagc gctcgacctc tacatggacc tcccgctcgc gccatgggaa
     1261 gcggtgctgg gagcgagcgt cgacgtgccg acaccggccg gccgcgtgac gctcaaggtg
     1321 ccgcccggca cgcacgcggg acagaagctg cggctttccg gacgcgggct gtcgcgcccc
     1381 gacggcacgt cggggcacct ctacgcggtt gcgcagatcg tggtgccgac ggtcgtggac
     1441 gagcgccagc gcgagctctt cacacaactc aaggacgcgt cggcgttcaa cccgcgggcg
     1501 cacatggtgg attgatgaaa atcgaagcga ccacggcgct cgtgctcgac gacacgggca
     1561 cgctcacgat tgcggaactc gtcgagcagt cgggcctctc ggcagacgag ctgcaggtgc
     1621 tcgtcgattg cggcgcactc gagccacgcg aaacggtggc accatcgtgg cgcttcagca
     1681 cgcgctgcgt cgtcaccgcg cgcacggcac ggcgtctgcg cgacgatctg gcgctcgacg
     1741 acacgcacgc gcttgccatc gtcctgcgct tcatgcagcg gatcgaggcg ctggagcgcg
     1801 aattgcgccg caggggctga tccgagcgtc acccatcgtc ctgcgccagc agtgccgcat
     1861 tcccgcccgc cgccgccgta ttgatcgaca gcgtgcgctc gacggcgaag cgcggcagat
     1921 aattcggccc gcccgctttc ggaccggtgc ccgatagccc ctcgccgccg aacggctgca
     1981 tgccgacgac ggccccgatc atgttgcgat tcacgtacac gttcccgacg cgcacgcggg
     2041 aaacgatgcg ctcgatcgtc gcatcgacgc ggctgtgaat gccgagcgtc aatccatagc
     2101 cgaagccgtt gattgcatcg acgagcgcgt cgagctcgct cgcgcgccac gtcgtgacat
     2161 gcacgaccgg accgaacact tcctgcgtca gccgatccat gcgatcgagc acgatcagcg
     2221 tgggcgcgac gaacgttccg ttcccacagg tcccgggcaa cgcgacccgg tgacgcacga
     2281 gccgcagcga atccatcgcc gcgacgtgtg cgtcgagcgc ctcgcgcgcc gcttcgtcga
     2341 tcaccgggcc gacgtcggtc gcaagatcgc ccgggtcgcc gatcacgagc tcgtccatgg
     2401 cgccttcgag cagctcgagc acgcgcggcg caatgtcctc ctgcaggcac agcagacgca
     2461 aggccgagca gcgctggccg gcgctgtcga acgccgactg cacgatgtcg gccacgacct
     2521 gctcggcgag cgccgagctg tcgaccacca ttgcattctg tccaccggtc tcggcgatca
     2581 gcggtacgat cggcgcgcgt gccgcgagcg attgcgcgat ggcgcttccg gcggcagtcg
     2641 aaccggtgaa cgcgacgcct gccacgcgcc gatcggcgac gagccgcgcg ccgatcgttt
     2701 cgccgggtcc cggcagcagc gccagcacgt cgtcgggcac gccggactcg tgcaacgtgc
     2761 gcacgacctg tgctgcgatc agcggtgtct gctcggccgg tttcgcgacc acggcgttgc
     2821 cggcggcgag cgccgccgcg atctgcccgg tgaagatcgc cagcggaaaa ttccacggcg
     2881 agatgcaggc gaacaccccc cggccgcgca gtgcgagcgt gttcgattca ccggtgggcg
     2941 agggcagcgc gatcggcgac gcgaagcgcg cccgcgcctg cgccgcgtag tagcggcaga
     3001 agtcggcggc ctcgcgcact tcggcaatgg cggcgctgcg cgtcttgccc gcttcgcggg
     3061 caagcatcgc cacccacagc gcgcgctgcg cttcgatacc ggcggcggcg cggtcgagga
     3121 ttgccgcacg ctcggatgca gcacgcgcgt cccatgcggg ctgggcggca acgagctgcg
     3181 caagtgcgcg atcgacgtcg gtctcgtcgg cgtcggttac cgtaccgatg cacgccctgc
     3241 gatcggccgg cgcgaatgcc tgccgtgccg cgcgctccgc ttcgccgatg ccgggtgctg
     3301 ccgtccagtg ttccgatgcg ctttgcacca gcgcgtcgtc cagcgcccgc atcgcctgct
     3361 ggtcggcgag ggaaacgccc tccgagttcc gccgttcggg cgcgaaaaga tcgatcggca
     3421 gcgggatgtt cgacgcaggc gcgaagtcgc cgttgcgggc gcgtgcgagc ggatcggcga
     3481 tcacgtcgtc gaccgcgatg cgcggatcgc cgatgcggtt gacgaacgac gtgttcgcgc
     3541 cattctcgag caggcgccgc accaggtagg gcaggaggtc acggtgcccg cccaccggcg
     3601 cgtagacgcg acacgcgatt ggaagatcgg ggtcagagcc gtaattattc gccgcgtccg
     3661 gcagcgagtt ctgggcatat cgcggtgacg aataattacg gctgtgaccc cgatctcgcc
     3721 gatctcgcgc gagaacgtgc cggtacagcg cctcccccat gccgaacagg cgctggaatt
     3781 cgtactcgtg gatacctcgg cgtgccgcgt gctcgctcac ccaagcgatg gtgtgcgcgt
     3841 tgtgcgtcgc gaacatcggg tagaccgcat cgccggcgtc gagcagcgct ttcgcgcatg
     3901 cgaggtagca gatgtcggtg tgcggcttgc gcgtgaacac cggatagccg gcgaggccct
     3961 ggacctgcgc gcgcttgatc tcggtgtccc agtacgcgcc tttgacgagg cgcaccggga
     4021 tgcgcgtgcc ggtgtggcgc gccagcccgg cgacgtaacc gacgacggcg cgtgcgcgct
     4081 tctggtacgc ctgcaccgcg agcccgaggc cgtgccaatc ggcaagtgcg ggatcgcgtc
     4141 gaacgcgcgc gaaaagctcg agcgatagct ccaggcgttc ggcttcctcg gcatcgatcg
     4201 tcattgcgat ctgcgcgtcg cgtgcagcct gcgcgaggga aatcacgacc ggcacgagct
     4261 cggcgagcac gcgtctgcgc tgcggatatt cgtagcgggg atgcagcgcg gaaagcttca
     4321 cggagatgga gggcgcccca acgatggcgc catcgttggc ggcccttcgc tgcgcgtaag
     4381 aacctatcgt cgcgatcgcg tgccggtacg cgtcgaagta gcgcgccgcg tcggccgcgg
     4441 tcaccgccgc ctcgccgagc atgtcgtagg agtggcgcca ttgcgcgtcg ctgttcgcaa
     4501 ccgcctcctc gatcgtgcgg ccgagcacga actgctccgc catcaccttc atcgcctggc
     4561 gcaccgcgac gcgcacgacc ggctcgcccg cgcgcgccac gaagcgctcg taccatgcac
     4621 gcgggtcgcg cgcgtcggcg ctgtcgatgc gcgtgagctt tccggtgagc atgaggcccc
     4681 aggtcgacgc gttgacgagc agcgaatcgg aggcgcccag atgatgttcc caatcgccgc
     4741 gcgagagctt gtcgcggatc agcgcatcgg cggtggcggc atcgggaatg cgcaacaacg
     4801 cctcggcgat gcacatcagc agcacgcctt cgggtgtcga caggtcgtac tgctgcagga
     4861 acgattcgat gccgccgtgc gactgccgat cggcgcgcac cgattcgacc catgcgcgcg
     4921 ccttctgcgc gactgcacgc tcggcgcgct cgtcctgcgc gatctgcgac gcgagcgcga
     4981 tcagcgtcgt gcgctcgtcg gcgaggaagg cgtcgcgaat cgccgcgccc gctgccgaat
     5041 cgggaatcaa tgcgctctcg gacacgatcg cttgcgcggg cggatgcatg gcgtaagcga
     5101 gcgtcagcct cgcggatgat gcgccgcatg caactgcttc agccgctcgc gcgcgacatg
     5161 cgtgtagatc gtcgtcgtcg tgatgtccgc gtggccgagc agaagctgca cgacgcgaag
     5221 gtcggcgccg tgattcaaca ggtgcgtcgc gaacgcgtgg cgcagaacgt gcggcgaaag
     5281 cgatgccttc gggatggccg cgcgcaccgc gtagcgcttg accagtgccc agaacgcctg
     5341 gcgcgtgagc ggtgcgtgcc gctgcgtcag gaacacctcg tcgcgcttcg attcgccggc
     5401 gagcgccggg cgcacctcgg caaggtagcg cgagatccac gcaaccgctt catcgcccat
     5461 cggcacgaga cgctccttgc tgcccttgcc gacgacgcgc acgacgccgg catcgtgcgc
     5521 gacctgcgac agcttcaatc cggtcagctc ggacacgcga agcccggtcg cgtagagcgt
     5581 ctcgagcatc gcgcgatcgc gaacgccgag cgggatgcgg gtgtcgggcg cggcgagcag
     5641 cgtttcgacc tgcgcttccg acagctgctt cggcagccgc cgcggcttct tcggcgcccg
     5701 cacgcgcagc gtcggatcct cggcgatcgc tccttgcgcg agctgaaagc gatagaagcg
     5761 acgcagcgcc gacaggcgcc tcgccaccga cgttgccttc gccttcgctt cgaactgatc
     5821 ggcaagccag cgctcgaaat cgacgcgcgc ggcgcgaagc agtgccgtgt cgcgcttcgc
     5881 gagccacgcg caccatccgg cgaggtcgcg gcgatagctt gcgagcgacg acgccgcgag
     5941 cccgtcctgc agccagagct ggtcgcagaa cgcgtcgatc atgccttcgt cgttcgtcat
     6001 gcgtcacatc ttcatgtgct tcggcagcag cgtgacgagc gacgggaaga accagatcgc
     6061 gagcaccgcc gcgcacatca ggaagaacat cggcagcgca tggcgggcga tccatgtgat
     6121 ctgccggttc gtcatgccct gcagcacgaa caggttgaag cccacgggag gcgtgatctg
     6181 cgccatctcg acgacgagca cgacgaagat gccgaaccac agcaggtcga tgcccgcttt
     6241 ctcgacggtg ggcaggatca cgcccatcgt cagcacgacc atcgagatgc cgtcgaggaa
     6301 gcagccgagg acgatgtaga agagggcgag tgccacgatc agcgcgaacg gcgaaagccc
     6361 gagcgtcgcg atccattcgg ccagatcccg cggcagcccg atgtagccca tcgcgagcgt
     6421 caggaatgcc gcgccggaca ggatcagcgc gatcatgcag tagaggcgcg ttgcaccgag
     6481 aaggctgtcg cgaaacgtcc gccagccgag cgagccctgc gtagcggata gcaccagcga
     6541 tccgacgacg ccgaacgctg ccgcttcggt tgccgtcgcc acgccggtgt agatcgaccc
     6601 cagcacgagc acgatcaaca gcacgacggg aatgaggtgt cgcgacgcgt gcagcttctg
     6661 cgcgaacgtc gtgcgctcgg tggcggccgg cacgagctgc ggattgacga gcgcccacac
     6721 gacgatgtag ccggagaaaa gcaccccgag aagaatgccg ggcacgatgc cggcaatgaa
     6781 caattgcgcg atcgacacgt tcgccgacac gccgtacacg atcatgatga tcgacggcgg
     6841 gatcagcagg ccaagcgtcc cggcgccggc gagcgagccg atcgtgatgc cgtcgggata
     6901 gccgcggcgt gcaagctcgg gcagcgtcat cttgccgatc gtcgcgcacg tcgccgccga
     6961 cgagcccgac acggcggcga agatcgtgca gccgatgatg ttcgtgtgca gcagccgccc
     7021 gggcacacgt tcgagccagg gagcgagtcc cttgaacatg tcgtccgaca agcgcgtgcg
     7081 gaacaggatc tcgcccatcc agacgaagag cgggagcgcc gtcagcgtcc acgacgatgc
     7141 ggtgccccag atggtgacgg ccatcgcatc gccggccggg cgcgacgaga acagcgtcat
     7201 gccgatccat gcgacaccgg ccagcgcaag cccgatccac acgccggtgc cgagcagcgc
     7261 gaacagcgca acgatgagca gcgtggtgat cagtgcgtcc atcgctgtcc cgggccggca
     7321 cgcgcgatcg catcattcat gatgcagcgc ctcggccggc atccgcgtgc ggcgcgttcc
     7381 ccgccattcg ccgatcagct cgtcgacgaa cgcgatgaac agcacgacgg tgccgacggc
     7441 catggcgatt tgcggtatcc acaacggcgt ggcgtcgttg ccggtcgaga tgtcgctgaa
     7501 ctgccatgat tgcacggcga gccgcaaggc gtagaaggcg aaggcccctg cgagcaccgt
     7561 tgcgaccgcg agcgcccaca actcgaggcc gcgccgagcg cggacgccgg cgtgctcgag
     7621 cagcagcgac acgcggatat gctcgccttg cttcagcgtg tgtgcaaggg ccaggaagcc
     7681 gctcgcggcc atgcagtagc cggcgtacga atcggtgccg gggatgaacc agttcatcag
     7741 ccggccgacg atgccgagca gcaccatcgc aagcgtgccg acgagaaaaa acgccgccac
     7801 ataagcggcg gcgtcataga gccagtccag gaatgcgcgc atgcgcgaga ggcgaagccg
     7861 tcgctattgc gtcgcgcgat actggtcgat gatcctcttc ccttcgggtc ccgccttgcg
     7921 cagccagtcg gcgagcatca cgttgccgac cttgcgcatg tcggcggtga gctgctcgct
     7981 cggcttgtcg atggccatgc ccttctggcg cagctggtcg acgtaccagc cggtcttttc
     8041 ctccgacagc ttccagccgc gcgtctcggc gtcggcggcc gcctttagca ccgcggcctg
     8101 cgtcgtcttg tcgagcgcat tgaacgcggc gcgattgacg atgacggcgt tcttcggcag
     8161 ccaggcctgc gtgtcgtaga agtgcttgag gtgctcgtat gtcttcgagt cgtagccggt
     8221 ggcgccggac gacatgtacg agtcgatcac gccggtcgca agcgcctgcg acacttccgc
     8281 ggcctgcacg gtcacgggct gagcgccgac gagctcggcg atcttcgatg tcgccggact
     8341 gtacgcgcgc catttgagcc ccttcatgtc ggcggcgctg ttcagcgtgc ggttggtgaa
     8401 gatgccctgc ggtggccacg gtaccgtgta gagcagcatc atgccctgct tcgcgagctt
     8461 gtcgtcgagc gccttgcgct cggccttgta cagcttcagc gcttcgctgt acgacgtggc
     8521 gaggaacggc acgccgtcga ttccgtacag cgggtcctcg ttctcgaaat tgacgagcag
     8581 gatttcgcca gcctgcgcct gcccaccctg caccgcacgc ttgatctcgg gcgccttgaa
     8641 gagcgacgcg ttcgggtgga gctggatctt gagcttcccg ccggtcgcct tgtcgacgtc
     8701 gccggcgaac tgctgcaggt tctcggtgtg gaaattgttg gcggggtagg ccgtgggaag
     8761 atcccacttc gtctgcgcga atgcgggcag cgcgagcgtc aggccaagca cgagcgtggc
     8821 ggcgaggcga ttcatgggtg ctccgggtat caggtataag aaatcaggga tcagggatca
     8881 gggatcggaa ggagcgactg gggcagtggg tgatttccgc gcagcatatt acaaggatcg
     8941 gccgaatgcc gggtaaccgc ctgcggagcg tcgctccata taatgtcgcg gcccgggtgc
     9001 accgcgccga aacgcttcgc tgatccctga tccctgatcg ctgacaccga tgcaattctg
     9061 gatcgatcgc ggcggcacgt tcaccgacat cgtcgcgcga cgcgccgacg gcacgctcgt
     9121 cacgcacaag ctcctgtccg acaacccgga gcgttatcgc gatgcggccg tgcacggcat
     9181 tcgtgaatta ctcggtctcg cgccgggcgc accgattccg ccaggcgcga tcgaggcggt
     9241 caagatgggc accaccgtcg cgacgaatgc gctgctcgag cgcaccggag agcggacggt
     9301 gctcgtcatc acgcgcggct tcgccgacgc attgcgcatc ggctaccaga accggccgaa
     9361 gctcttcgtg cgcaggatcg agctgccgtc gcagctctac gagcgggtga tcgaggccga
     9421 cgagcgcatc ggcgcccgtg gcgagatcgt gcgtccgctc gatctcgaga cgctcgagcc
     9481 tgcgcttcgc gaggcgttcg ccgacggtgt tcgcgcggtg gccatcgtgc tgatgcatgg
     9541 ctaccgttat ccgcagcacg agtgcgccat cgccgagctc gcgctcgcga tcggctttcc
     9601 gcaggtttcg atctcgcacg aggtgagccc gctgatgaag ctcgtgtcgc gcggcgacac
     9661 caccgtcgtc gacgcctacc tgtcaccgat cctgcgccgc tacgtcgacg aggtggagcg
     9721 ggatctggtt ggcgaccgcc gtgacgaaac cggcgtcgtg cctccgttgc gcctgcagtt
     9781 catgcagtcg tcgggtgggc tcaccgatgc gcgccgcttc cagggcaagg acgcgatcct
     9841 gtcgggaccc gcgggcggca tcgtcggcgc ggtcgaggtg tcgcggctcg ctgggttcga
     9901 gcgcatcatc ggcttcgaca tgggcggaac gtcgacggac gtcacgcact gggccggcga
     9961 atacgagcgt gcgttcgtca ccgaagtcgc cggcgtgcgg ctgcgtgcgc cgatgatgag
    10021 catccatacc gtcgccgcgg gcggcggatc gatctgcgcg ttcgacggtg cgcggctgcg
    10081 cgtcggtccg caatcggccg gtgccaatcc gggccccgcg agctatcggc gcggcgggcc
    10141 gctgacggtg accgattgca acgtgatggt gggcaagctc gatccggagc tgtttcccaa
    10201 tgtgttcggg ccgcgcggcg acgagccgct cgacgccgcg atcgtgcgca cgaagttcga
    10261 agcgctggcg gcgcaaatcg catcgtcgac cgggcacgcg cgaacgcccg agcaggtggc
    10321 cgacggtttt ctcgacatcg cgatcgagaa catggcgaac gccatcaagc acatctcggt
    10381 gcagcgcggc tacgacgtca ccggctacac gctgtgctcg ttcggaggcg ccggaggcca
    10441 gcacgcctgt cgcgttgccg atgcgctcgg catgtcgcgc gtattcattc atccgctcgc
    10501 cggtgtgctg tcggcgtacg ggatggggct cgccgatgtg cgcgcgctga agcagcaggc
    10561 cgtcgagacg acgctttcgc cggcagcgct cgacgcgacg cgcgttgcgt tcgcgatgct
    10621 cgaggacgcg ggacggcgcg acgtcgaggc gcagggcatc gcaccggctc gcattcgctg
    10681 cgagcgcacg ctgcagctca agtacgaagg cacggatacg acgctgggcg tgccgaccgc
    10741 ggcgggcgtg gacgcggcga tcggcgagtt cgagcgacgt taccggcagc aatatggctt
    10801 cctgatgccc ggcaagccgc tcgtgatcga agcgctcgcc gtcgaggcca tcggcaccgc
    10861 gcacgacatc caggacgagc gccgcgactt tgcgccgcgg cccggcgagc tcgcaccgtt
    10921 ccgccgtgcg cgaatccaca cgcagggcga ctggcgcgat gcggccgtgt atgcgcgcga
    10981 ggaacttcgt cccggcgatg cgatcgacgg cccggcgatc attcgcgagc ggaacgcgac
    11041 gacggtgctc gaacccggat ggcgagcgac gctgaccccg cgcgatcacc tgatcctcga
    11101 gcgcgtcgag ccgatccgcc gcgcgcacgc gatcggcacc agcgccgacc cggtgatgct
    11161 cgaggtcttc aacaacctgt tcatggcgat cgccgagcag atgggcgtga cgctcgccaa
    11221 cacgtcgtat tcggtcaaca tcaaggagcg cctcgacttt tcgtgcgcac tgttcgacgc
    11281 cagcggcaac ctgatcgcca acgcgccgca catgcccgtg cacctcggct cgatgggcga
    11341 gagcgtggcg acgatcatcg ggcggcgcag cggcagcatg cacccgggcg atgtgttcgt
    11401 gctcaacgcg ccgtacaacg gcgggacgca cctgcccgac gtgacggtga tcgcgccggt
    11461 gttcctcgac gcgggcgcga aaaaggggcc agggtcgatt tctatggaaa cgatgactga
    11521 gggcgatgtt cgcaagaaat cgaccctggc ccctttttcg cgatttgcgc ccgagttctt
    11581 cgtcgcctcg cgcggtcatc acgccgacat aggcggcatc acgccgggct cgatgccgcc
    11641 cgactcgacg cacgtcgacg aggagggcgt gctgctcgac aacgtgcagc tcgtcgccga
    11701 cggccgcttc ctcgaggccg ggatgcgcgc gattcttctg tccggccgct atccggtgcg
    11761 caacgtcgag cagaacctcg ccgatctgcg cgcgcaggtt gccgcgtgcg cgaagggcgc
    11821 cgaggagttg cgcaagatgg tcgcgcactt cggcctgccc gtcgtgcgcg cctacatgaa
    11881 gcacgtgcag gacaacgccg aggaggcggt gcgccgcgtc gtgggtgcgt tgtccgatgg
    11941 aacgttcgcc tacgagatgg acaatggcgc ggtgatccgc gtcgcgatca aggtcgaccg
    12001 cgcggcgcgc tcggcgacga tcgacttcgg cggcacgagc ccgcagcagc cgacgaactt
    12061 caacgcgccg tcggcggtgt gcaaggcggc ggtcctgtac gtgtttcgca cgctcgtcga
    12121 cgacgacatc ccgatgaacg ccggctgtct gaagccgttg tcgatcgtga ttccggaagg
    12181 ctcgatgctc aatccgcgct atccggcggc cgtggtcgcc ggcaacgtcg agacgtcgca
    12241 gacgatcacc gacacgctgt acggggcgct cggcgtgctc gctgcatcgc aggggacgat
    12301 gaacaacttc acgttcggta acgatcgcta ccagtattac gagacgatct cgggcggcgc
    12361 gggcgcgggc gccgatttcg acggcgcgag tgtggtgcag acgcatatga ccaactcgcg
    12421 cctcacc
//
LOCUS       DASSVX010000042        24666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_684700, whole genome shotgun sequence.
ACCESSION   DASSVX010000042 DASSVX010000000
VERSION     DASSVX010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 24666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24666
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684700"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFO53_01975"
     CDS             complement(<1..218)
                     /locus_tag="VFO53_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8875070.1"
                     /translation="MAGATLSIVNPATGSTVVRLAADGARDVAQRHAAARAAQPRWAA
                     TPIRKRIATIERFREQIVTQGETLARTLT"
     gene            complement(218..1102)
                     /locus_tag="VFO53_01980"
     CDS             complement(218..1102)
                     /locus_tag="VFO53_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET8875071.1"
                     /translation="MAAPLLIGVSARIHHPSDNVVKLGGVYTKTLHYLEQSVTHWVMS
                     KNVLVMMIPAIESEGMIQRSEMRLYHYAEALDGLVLQGGADIAPPSYGEAPRDPAWAG
                     DRVRDRYEIELFEAFVGKGKPVLGICRGCQLINVAFGGTLYQDIATQVPGALVHRDIA
                     KYERHFHTLKFVPDTSLAKLYPGRNNATINTIHHQAVNKLGRGLIVEAVAEPDGIVEA
                     IRWRGPSYVRGVQWHPEFMVGPGIGEEHLDGAPILNEFLEAARQRKVKRGFLRRAIPA
                     LRSARSVDAASAKRGKAP"
     gene            complement(1109..2278)
                     /locus_tag="VFO53_01985"
     CDS             complement(1109..2278)
                     /locus_tag="VFO53_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET8875072.1"
                     /translation="MSQHRFAFPTPIVFGPGARREVAAHLIAAGCARPLVVTDRGLAA
                     LPVFAAFQRELAGVEHAVFSGIWGNPLRSQVMEGARAYRAHAADSIIGIGGGAALDVA
                     KAVAVMATHPGDILEYAWDHPQVRPISEPLPHFVAVPTTAGTGSEVGRSSVIGDDVTH
                     IKKIVFSPALLAKAVFADPELTLDLPPAITAATGMDALTHNVESYLSPAWHPLCDGIA
                     VEGVRIAARALPAAVANGHDVAARGDMLMCSMMGAIAFQKDLGAVHSCAHALSTVADL
                     HHGLANGIMIDHVMRFNLPAAAAKLAELARVARVPGGERGSDEARAHAFVDWLARLKR
                     QLGIPASLSDYAGARRVGMDDIAKLTEVAVADICHQTNPRPCTADDFRRIFADAM"
     gene            complement(2292..3665)
                     /locus_tag="VFO53_01990"
     CDS             complement(2292..3665)
                     /locus_tag="VFO53_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET8875073.1"
                     /translation="MPASAVAADILAAVRKSPGNRVKVAVSDIDGILRGKYLHKDKFE
                     SALEAGFGFCDVVYGWDCHDQCYDNTTYTGWHKGFPDALARIDVGTHRKVPWDDGVDF
                     FLGEFVIRKDGRDAPFPLDGRQVLKRVLKRAEKLGVVPMCGLEFEWFNFIETPQSWAD
                     KQGVGPTPLTPGMFGYSLLRANHSRDYFKALAEEMAAFGVPIEGLHTETGPGVFEAAI
                     MFSEALEAADRGLLFKAGAKEIAARFGIMPSFMAKWSQQYPGCSGHCHQSLSDGKKNL
                     FHDAKSRSGMSKLFESYLAGQLATLLEFAPMLWPTINSYKRLVDGFWAPVKPTWGLDN
                     RTASFRVIAGSPKSTRLETRAPGADVNAYLAVAACIAAGLDGIERKLALTTPPIEGTN
                     RGGEDIPRAPRTLIETTRVFRQSERARDWFGDDFVDHFAATREWEWRQWQDAVTDWER
                     KRYFEII"
     gene            complement(3779..4186)
                     /locus_tag="VFO53_01995"
     CDS             complement(3779..4186)
                     /locus_tag="VFO53_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875074.1"
                     /translation="MHARRIFRWFAGQTWTNEDYSGDYLGGYSITNTNCFPANGNGIE
                     EVAALLAVAQNGSAMSITATTSVATCTFGGTYSQTGKLGQVNGNYSCTSGVQGSFALV
                     EMTPTISGFTGRIVGQNQFCDFAGALGGIRRAQ"
     gene            4352..5578
                     /locus_tag="VFO53_02000"
     CDS             4352..5578
                     /locus_tag="VFO53_02000"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8875075.1"
                     /translation="MKLLLDRFALFYPMRIVGTAFMVIVATLYAWHRDRFGAFYYGLV
                     LVLVVYPHVVHHVARKYPQNRRAIELRTFVFDSFAVGVVVHSIGFGPFPTFVLITVAL
                     ASALSVDGPRQSLLSAAAVAAGIVAYAVFFPVNTAFVDTIAVDIVSSAFTFVYFMAFA
                     YSARNRSALLLRSEAELRERTASLEIEKLRSDRLLFNLLPSGLAAQMAMPGGIRPARF
                     ERVVLLGIELRHFSRMLQSSPEDEALAHLMHCFKAFDAIADRFGMEKLKTMGDVYIAI
                     GGLPVSAPGDVAAGIRAASSAREFLRDLAESRRAHGAFVLDARIAVHAGSVIGGIVET
                     SKLSYDVFGDAIRTLFGLLRGCEDGAIIVSDAARCLAGAGFERPDIETAGSAAASLSH
                     ADGARSHAPSDAATQA"
     gene            5586..7439
                     /locus_tag="VFO53_02005"
     CDS             5586..7439
                     /locus_tag="VFO53_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET8875076.1"
                     /translation="MDDEFIQDLRSLLPRFLGPLPDPELTAFAARLQRELVASGTLLY
                     RQGEASDCMHFVVTGRLEVWTHDRYGDRRLVAHLSSGDCVGELSLLTGDTRAADVVVL
                     RDAVLARLGRDDYDAMLRRYPEAGLNIARFALRTLRQGTTFVPKVRNIALVPLGSGVR
                     VAEFGRRLELALLRFGSTLYLDSPVAHARLAASRAGPAHAATSERHLDRLLDEAEHAR
                     RFVICEADAECTGWTRKCVAHADRILFVADAAQSPALSAIEEALSGAAPGASLTQRDL
                     VLLHANRRVPPAGTAAWLAPRTGYRHSHVAWEGNRDFHRLARLLSGNAVTLVLGGGGA
                     RGLAQIGVIRAIREAGVPIDAVGGTSVGAIIGALVALDWMDEEILQSCKHAFVDDRPL
                     DDVTVPVFSLLSGKKFARTLHHYIGDVDIEDLWQPYFCVSSNLSESRVHVHHAGRLWE
                     AMQASAALPGMLPPMVSNGQLYVDGGVLDNLPVGVMKRFIGGNTIAVEATVKVEYSID
                     AAEFPSPLAYLRSRLSRRGRYALPTLPSLLIKSTLLGGGSGRNELRDGSDLYVNPPMR
                     EFGFLDWHRIYTMVDVGYRHGQQCVRAWLEVHPELQRRDESPGLPRTAMAL"
     gene            7453..8301
                     /locus_tag="VFO53_02010"
     CDS             7453..8301
                     /locus_tag="VFO53_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8875077.1"
                     /translation="MPIGAGRTSALLALHVAVALFGFAALFGKWIALSPVAIVLGRTV
                     IAAATLALLVWMRHRPLGRPERALLVNGPLLALHWVSFFAAVQIASVAIALFGYASFP
                     VFVLLLERGRRASGGEYATALLAALGLAMLAAHLSWSGAGMRGLAIALVSGVTFALLV
                     VRNRRLVAGGSAARIAMWQNLAAGVCLLPIVALADRPAAWPRFADAALLLVLGVVCTG
                     LAHTLFIASMRRVSAHSASVVASLEPVYGIALAAWLLGEKPDARTMGGGVLIVVAALI
                     ASRRVE"
     gene            8417..8911
                     /locus_tag="VFO53_02015"
     CDS             8417..8911
                     /locus_tag="VFO53_02015"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HET8875078.1"
                     /translation="MIVVLMGVSGSGKSTVGVALAAALGWRLIDADDLHSPHNVAKMA
                     AGIPLTDDDRWPWLDRVVDELRRARAEGTNVVLACSALKQRYRDRLAQAGDVRFVYLR
                     GDAQTIAARLASRHHRYMPAALLESQFATLEAPSDAIDVDITQSVDAQVATIIAALSD
                     GKAP"
     gene            8908..10089
                     /gene="metC"
                     /locus_tag="VFO53_02020"
     CDS             8908..10089
                     /gene="metC"
                     /locus_tag="VFO53_02020"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET8875079.1"
                     /translation="MTGGSRYRIATEVAHLGRDPRRFLGAVNTPVFRASTILFRTVAE
                     LEQAARGEFPDLAYGLHGLPTVSDLARALAALEGGHAALIVPSGLAATTFPLLALAKP
                     GDHVLVTDAVYGPTRRFCDNHLRRLGIDVSYYDPLLGAEIAREFRPRTTLVFTESPGS
                     LTFEVQDIPAIAAVAHARGARVILDNTWATPLGFRSFDHGVDVSVHAVTKYIGGHSDV
                     LLGAVIASAEAYPPLARLWTDMGITASSDDCFLGLRGLRTLAVRLARHAASAMEVARW
                     LAQQPEVREVLYPALPEDRGHALWARDFHGASGLFGVVLEPADRARIAAMLDGMRLFG
                     MGWSWGGFESLIIPTWPERARTATSWDAGGPMLRLAIGLEHPQDLIDDLDDGLRRLRG
                     V"
     gene            complement(10086..10487)
                     /locus_tag="VFO53_02025"
     CDS             complement(10086..10487)
                     /locus_tag="VFO53_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET8875080.1"
                     /translation="MLDASIALAWFLPDETSASARELLVRLEQEDAWAPSIWQLEIAN
                     ALVMAERRKRISESNRKEFLEHLAVLPVSLDQPHTAAGLPALSEICRTYALSIYDACY
                     LQLAQSRRVALATFDRELRAAAARSGVATLP"
     gene            complement(10496..10768)
                     /locus_tag="VFO53_02030"
     CDS             complement(10496..10768)
                     /locus_tag="VFO53_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HET8875081.1"
                     /translation="MNEIGSYEAKTHLPALLERVERGERFVITRHGRPVAELGPVARR
                     DPQTIHAAVTAIDRLRNRLAKRGVTLRDALGNPERIRDGLHEGHHY"
     gene            complement(10867..11628)
                     /locus_tag="VFO53_02035"
     CDS             complement(10867..11628)
                     /locus_tag="VFO53_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8875082.1"
                     /translation="MDLSLAGRHALVTGANGSLGGHFARTLAKAGANVTMTARGVDAL
                     DPLVDEIAKLGRRAVPLALDVTDAASVARTFDHAAHALGAITVVVNNAGIAITKPLLD
                     HTEDDWLRVIDVNLNGAWRVAQAAARHMVAHGAGGSIVNVASILGLRVAAQVPSYAAS
                     KAALIQLTKAMALELARHRIRVNALAPGYVETAMNHDFFASDAGQAMIKRVPQRRIGM
                     PDELDGALLLLASDAGSYMTGSVVTVDGGHVASSL"
     gene            complement(11644..12015)
                     /locus_tag="VFO53_02040"
     CDS             complement(11644..12015)
                     /locus_tag="VFO53_02040"
                     /inference="COORDINATES: protein motif:HMM:NF040046.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HET8875083.1"
                     /translation="MPDAAALLATARETLASELLPALSGELRYRGLMVANALAIAARE
                     MASGHDAIRREIELLRPLAAQPVPPSDAGSDDVQALRRRVAAAIRDGRFDDDVHAKTL
                     LAALDEIAVQRLAISNPKALR"
     gene            complement(12066..13088)
                     /locus_tag="VFO53_02045"
     CDS             complement(12066..13088)
                     /locus_tag="VFO53_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET8875084.1"
                     /translation="MTASDVAGADTLAAWLRAETGADDVRVTRFERLRGGAIQHNYLL
                     DVDIRGGGWQGRQSWVLRTNARSRVDASASRASEYEVLRTAYAAGVHAPKPLFLCTDP
                     AVLGSEFFVMERLSGVAAGHRITRDAALVPDPPALAYELGANLAKLHAIRPSPRSSAT
                     NGRGSSGNPALEGIATYRAWLDRLDGAYPILEWGLRWCERHAPSTYEVTFVHRDYRTG
                     NYLVDGGKLAGVLDWEFAGWGDPREDLGWFTARCWRFARRDLEAGGIAALEPFIAGYA
                     SVAGHGISRDDLDYWQTMAHLRWALIALQQAQRHRDERSLELALTGRIVDELEYEVLQ
                     LTQRRK"
     gene            complement(13094..14287)
                     /locus_tag="VFO53_02050"
     CDS             complement(13094..14287)
                     /locus_tag="VFO53_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8875085.1"
                     /translation="MDFEIPPELAGLQARIRRFIADEIIPLESDARRTRHGPSEELRR
                     ELIDKAREAGLLAPHVAKEYGGLALTHVGRAIAFEEAGYSPLGPVALNVFAPDEGNMH
                     LLEAVATPAQKERWLRPLAAGTIRSCFCMTEPAPGAGSDPSMLMSTATRDGGDYVING
                     AKWFITGADGAAFAIIMAKADDAGATMFLCDMNTAGIIVERAMDALDECFPGGHAVVR
                     FDGLRVPADAVLGEVGKGFRYAQVRLSPARLTHCMRWLGAVRRAHDVAVDYAKRRHAF
                     GKRIGDHEGVGFMLADNEMDMHAARLTIWHCAWLLDRGELALHESSRAKVIVSEAAWR
                     VADRCVQVLGGQGVTNETVVARIFADLRAFRIYDGPSEVHRYSIARRILRDAPRRAGG
                     PPAAH"
     gene            complement(14377..15951)
                     /locus_tag="VFO53_02055"
     CDS             complement(14377..15951)
                     /locus_tag="VFO53_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET8875086.1"
                     /translation="MALEFNYESLTPTAFLRRAAYVFGDRVGIVDADTRFTYVAFYER
                     CRRMAGVLRAMGVAAGDRVAVLAPNTHVLLAAHYAVPFAGAVLVALNTRITPADIAYI
                     LRHAGVSVLIYDDDFRSTAEQALAEAAIDIRSLRAGGSNDELELAIANAEALEQKVAD
                     ERGLLAINYTSGTTARPKGVMYHHRGAYLQALAMAMQMGITSDSVYLWTLPMFHCNGW
                     CFPWAVTAMGGVHLCLRKLEPATIWRHLRESGVTHLCGAPTVLTMIAWDAAAAPVERV
                     ARIATGGAPPTPALLERMAALGLDVTHLYGLTETYGPAGICEWRSEWNGLRIEQQARL
                     KARQGVANAIGETIRVVDADGRDVPADATTMGEIAIRGNNVMLGYYEDDAATRKACPD
                     GWFRTGDLGVLHPDGYVELRDRAKDIIISGGENISSVEVEQALTQHPAVLEVAVVAAP
                     DDKWGEVPVAFVTLKEGEDASGDELIEHARGRLARFKLPKRVVFGVLPKTSTGKVRKS
                     ALRERLRSGADASGRS"
     gene            complement(15965..16708)
                     /locus_tag="VFO53_02060"
     CDS             complement(15965..16708)
                     /locus_tag="VFO53_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875087.1"
                     /translation="MIAPLLEVSAVSKSFDGVDAVRNVSFSVAAGEVVAMIGPNGAGK
                     TTLFNLIHGELVSDRGDVVFDGRSLVGQATHAIARAGIGRTFQVAAMFASMTVRESMA
                     LALAAHAERDAAVMSRLLPAVSAPADALLARLRIGDLRDAHCAGLAYGDAKRVELAMA
                     LAGAPGLLLMDEPTAGMTPRSRRDLMQLVVDLARADGRTVLFTEHDMDIVFGFAERII
                     VLDRGEIIAEGTPQAIRENRDVQAAYLGT"
     gene            complement(16705..18642)
                     /locus_tag="VFO53_02065"
     CDS             complement(16705..18642)
                     /locus_tag="VFO53_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875088.1"
                     /translation="MQSVAWGGPAHLTGPYVSFASLAVQILNGLASASSLFLLAAGLT
                     LIFGVTRIVNFAHGSLFMLGAYVAYSTATAFGAYAGSPLGFWAASLVAALAVALLGAL
                     IEVLLLKRLYPAPELLQLTATFAIVLIAGDAALALWGPEDRLGPRVPGLDGALPWLGQ
                     AIPEYDLLLLVVGPLVLIALTWIVRRTRFGMLVRAASEHRVLAAALGVDERTLFTAVF
                     ALGSFLAGLAGALALPREPAHLGMDLGVIADAFVVTVLGGLGSIPGAFIAAVIIGVTK
                     AMCIAVGTIHVGDVAVALPKLTLVIEFVLMALVLLVRPAGLFGTPASVPATTAVVAER
                     ALVQRPHRRSMMVAAVLVVAALLLPLARDAYLLVLATDIFIAALFTASLQLIVARGGM
                     TSFGHAAYFGVGAYATCLAATHGWPFPLALVAAPMVAALAAFVFGALAVRMSGIYLAM
                     LTLAFAQILWSVAIQWDTLTGGSNGITGAWPPEWLGERTRYFLFVLIVAGAALVALAG
                     IAFTPFGYALRGSRDSPLRAAALGIDVRARRMKGLVLAGAFAGLAGALFAFSKGGVAP
                     DALSIPRSVDALVMLLLGGQNALFGPLLGATAFTWLSDVLGRYTEYWRACVGVAILLI
                     VSVFPNGIGGGLLRLRTRPAR"
     gene            complement(18677..19879)
                     /locus_tag="VFO53_02070"
     CDS             complement(18677..19879)
                     /locus_tag="VFO53_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8875089.1"
                     /translation="MAHRLTSLLLIVLSAWASSAPNAVAQTIKVGEINEYKQFPAFLE
                     PYRKGMELAVDEINGAGGVLGKKIEVVSRDDNGVPGDAVRVAEELLTREHVALLTGTF
                     ASNIGLAIADLAKRRHVLFIAAEPLTDKIVWEDGNKYTFRLRASTYMQTAMLVPGAVK
                     LAKKRWAIVYPNYEYGQAATATFKAQMQAQQGPGLEFVEQAVPLGKIEPGAVVQALID
                     AAPDAIFSSLFGPDLARFVREGRLRGLFNGRPVFNLLGGEPEYLDPLKDEAPIGWYVT
                     GYPWYAIDTPEHKRFRDAYQAKFNDYPRLGSVVGYSAVKSAAAALARAGSTDTEKLVA
                     AMQNLTLETPFGQVTYRAIDHQSTMGAYVGRTDVRDGKGIMVDWRYADGKDYLPPDDV
                     VRRLRPAQ"
     gene            20028..23567
                     /locus_tag="VFO53_02075"
     CDS             20028..23567
                     /locus_tag="VFO53_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008389297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8875090.1"
                     /translation="MLRLAHGLEFADLYTVDGAARIDERFIAHLARADATVAERLLEA
                     RTAPAPLAGKDESALLIDIAPHLEDFLAELFGIEDAVRALEARHHELAPLFSVKRQFV
                     QRKAMNAYKADVAASFDGRALRGAIDALLGAHGDVASFELAFARAVISWQQDEAANAT
                     ALDVALRYAAWAAHAQVGRRAHRGGVLFRAPRKLDALRLVPVESVVQDGVAMLQHPHA
                     AGLRRRAGFTLTDAGTDLTGGLDQAHYCIWCHEQGKDSCAHGLPEKRARPAAIDAPDE
                     AAAHPYKQSVFGVTLAGCPLEERISEFHKLRAEGWALGALATICVDNPMVAATGHRIC
                     NDCMKACIYQKQDPVDIPQSETRVLKDVLALPYGFEIYSLLTRWNPLDLRRPKPLQAT
                     GKRVLVVGQGPAGFTLAHHLLNEGHTVVAIDGLKIEPLDPALSGVRPSGERVPFMPVA
                     DFASLTEPLDDRVMAGFGGVAEYGITVRWDKNFLKVIRLLLERRSNYALYGGVRFGGT
                     VDADDAFAMGFDHIALAAGAGRPTVLDLPHGLAHGVRTASDFLMALQLTGAAKDDSIA
                     NMQVRLPVVVIGGGLTAIDTATEALAYYPVQVEKFLARYEALVALGGRGGVAAAWSEH
                     EASIAAEFIAHAHAIRAERAQAAAEHREPALAELLRAWGGATIAYRKRLIDSPSYTLN
                     HEEVDKALEEGIRFAEGLTPLAIDVDDAGHAMALRVSVQTRTDEGVWIEQARARMPAR
                     TILVAAGTQPNTVLAREDATHFTLDGRYFRLLHEDGSLAQPVRGLAKPAQPAVLTHVG
                     DDGRAISFFGDLHPSFHGNVVKAMASAKQGYPIVSRLLARTDSVLRRPDTEFFAMLND
                     AFRARVQRVVRLTPTIVEVVVHAPAAARKFRPGQFYRLQNFESLAQRNEGTRLAMEGL
                     ALTGAWVDRERGLVSVIVLEMGGSSDLCVALEPGEPIVLMGPTGAPTEIEHDKTVVLV
                     GGGLGNAVLFSIGRAFRAAGSKVLYFAGYRRIEDRYKVDEIEAAADAVVWCCDEAPGF
                     AATRPQDRVFTGNIVEGMRAYAAGELGEQPIAFGDCDRIVAIGSDKMMAAVAAARHDA
                     LAPYLSPRHRAIGSINSPMQCMMKEICAQCLQPQRDPVSGRTRYVFSCFNQDQPLDDV
                     DFPALDARLRQNAVQEKLTARWVARVNAAAESD"
     gene            complement(23625..24497)
                     /locus_tag="VFO53_02080"
     CDS             complement(23625..24497)
                     /locus_tag="VFO53_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875091.1"
                     /translation="MRKSASVARSILRAAHRALPLAAGACIVFAANPASAQLAPNQTQ
                     GFGNGGLVTFTYLQNFDCVDEPQLDLDFNGIAAASDPNEMQTPICQAITEPSSDPSGG
                     DIAHTAHLYVLVPMFGSDTHPEDAMACPDGGRPNELCGTALGSALISLFGFVPEAWKT
                     TPAVTTQCPDPNHPVAGTCTMHASSVDLSVLLGALGKAPDPPTAPVFLPTPNHSHVVD
                     NSRVNARPIWWQVRPVLVMDPRDWPTADGSSGITSSTAMDAAEAAGRAVEVGSNFFLF
                     FSSRMAAAHATMQH"
BASE COUNT         3904 a         8343 c         8361 g         4058 t
ORIGIN      
        1 gtcagcgtcc gggcgagcgt ttcgccttgc gtcacgatct gttcgcggaa ccgctcgatg
       61 gtggcgatgc gcttcctgat cggcgtcgcc gcccagcgcg gctgcgcggc acgtgcggcc
      121 gcatggcgtt gcgccacgtc gcgcgcgccg tcggccgcaa gccgaacgac ggtcgacccg
      181 gtggccggat tgacgatcga gagcgttgct cccgccatta cggcgccttg ccgcgctttg
      241 ccgaggcagc gtcaacgctg cgcgcggaac gcagcgccgg aatggcgcga cggaggaatc
      301 cgcgcttgac cttgcgctgg cgcgccgctt cgagaaattc gttcaggatc ggcgcgccat
      361 cgagatgctc ttcgccgatg ccgggtccga ccatgaattc gggatgccat tgcacgcccc
      421 ggacgtaact cggcccgcgc cagcggatcg cctcgacgat gccgtccggt tcggcaacgg
      481 cttcgacgat cagcccgcgg ccgagcttgt tcaccgcctg gtggtggatc gtgttgatcg
      541 tggcgttgtt gcggccggga taaagcttcg ccagcgacgt gtccgggacg aacttcagcg
      601 tgtggaagtg gcgctcgtac ttcgcgatgt cgcgatgcac gagcgcaccg ggcacctgcg
      661 tcgcgatgtc ctggtacagc gtgccgccga aggcgacgtt gatgagctga cagccgcggc
      721 agatgccgag caccggcttg cccttgccga cgaatgcctc gaagagctcg atctcgtagc
      781 gatcgcgcac gcggtcgccg gcccacgccg ggtcgcgcgg cgcttcgccg tagctcggcg
      841 gcgcaatgtc ggcgccgccc tgcagcacca ggccgtcgag cgcctcggcg tagtggtaaa
      901 gcctcatctc gctgcgctgg atcatgccct cgctctcgat ggccgggatc atcatcacca
      961 gcacgttctt cgacatcacc cagtgcgtga ccgactgctc gaggtaatgc agtgtcttcg
     1021 tgtagacgcc gccgagcttc acgacgttgt cgctcggatg atggatacgt gccgagacgc
     1081 ctatcaggag aggtgcagcc atgcgttcct acatcgcgtc ggcgaaaatg cgccggaagt
     1141 cgtcggccgt gcacggccgc ggattggtct ggtggcagat gtcggccacc gcgacctcgg
     1201 tcagcttcgc gatgtcgtcc atgcccacgc gtcgtgcgcc ggcgtagtcc gacaacgaag
     1261 ccggaatgcc cagttgccgc ttcaatcgcg cgagccagtc gacgaacgcg tgcgcgcgtg
     1321 cttcgtcgct gccgcgctcg ccacccggca ctcgtgcgac gcgggcgagc tcggcgagct
     1381 tcgcagcggc cgccggcagg ttgaagcgca tcacgtggtc gatcatgatg ccgttcgcga
     1441 gcccgtgatg caggtcggcc accgtggaca gcgcgtgcgc gcaggaatgc acggcgccga
     1501 ggtccttctg aaacgcgatc gcgcccatca tcgagcacat cagcatgtcg ccgcgcgcgg
     1561 ccacatcgtg tccgttcgca acggccgccg gcaacgcgcg tgccgcgatg cgcacgccct
     1621 cgacggcgat cccgtcgcac agcggatgcc aggcgggtga cagatacgat tcgacattgt
     1681 gcgtcagcgc atccatgccc gttgcggcgg tgatcgccgg cggcaggtcg agcgtcagct
     1741 cgggatcggc aaacacggcc ttcgcgagca gcgccggcga gaacacgatc ttcttgatgt
     1801 gcgtcacgtc gtcgccgatc accgacgagc gtcccacctc cgagcccgtt ccggccgtcg
     1861 tcggaacggc gacgaaatgg ggcagcggct cgctgatcgg ccgcacctgc ggatgatccc
     1921 acgcgtactc gagaatgtcg cccggatgcg tcgccatcac cgcaaccgcc ttcgcgacgt
     1981 cgagcgcggc acctccgccg ataccgatga tgctgtcggc ggcatgcgcg cgatacgcgc
     2041 gcgcaccttc catcacctgg cttcgcagcg ggttgcccca gatgccggaa aagacggcgt
     2101 gctcgacgcc tgcaagctcg cgctggaacg cggcgaaaac cggcagcgcg gcaaggccgc
     2161 gatcggtaac gaccagcggc cgcgcgcatc ccgcggcgat gaggtgtgcc gcgacttcac
     2221 ggcgtgcgcc ggggccgaac acgatcggtg tcggaaaggc gaagcgatgc tggctcatga
     2281 gtgtcgcgcg ctcagatgat ctcgaaatag cgcttgcgtt cccaatcggt gacggcatcc
     2341 tgccattgcc gccactccca ttcgcgggtc gcagcgaaat ggtcgacgaa atcgtcgccg
     2401 aaccagtcgc gcgcgcgctc cgattggcga aagacgcgcg tcgtttcgat cagcgtgcgc
     2461 ggcgcccgcg ggatgtcctc gccgccgcgg ttggtgcctt cgatcggcgg cgtggtcagc
     2521 gccagcttgc gctcgatgcc gtcgagcccc gcggcaatgc aggcggcaac cgccaggtag
     2581 gcattgacgt cggcgccggg cgcgcgcgtt tcgaggcgcg tcgactttgg cgaaccggcg
     2641 atcacgcgga agctcgcagt gcgattgtcg aggccccagg tcggcttgac cggtgcccag
     2701 aagccgtcga cgaggcgctt gtagctgttg atcgtcggcc acagcatcgg cgcgaattcg
     2761 agcagcgtcg caagctgccc ggcgagatag ctttcgaaga gcttgctcat gccactgcgg
     2821 ctcttcgcgt cgtggaacag attcttcttt ccgtcggaca gggattgatg gcagtgcccg
     2881 gagcatcccg gatactgctg gctccatttc gccatgaagc tcggcatgat gccgaagcgg
     2941 gcggcgatct ccttcgcgcc ggccttgaac agcagtccac ggtcggcggc ttcgagcgct
     3001 tcggaaaaca tgatcgcggc ttcgaacaca cccgggccgg tctcggtgtg cagcccctcg
     3061 atcggcacgc cgaacgcggc catttcttcc gccagcgcct tgaaatagtc gcgcgaatgg
     3121 ttcgcgcgca gcagcgagta gccgaacatg cccggcgtca gcggcgtggg gccgaccccc
     3181 tgcttgtccg cccacgattg cggcgtctcg atgaagttga accattcgaa ctcgagcccg
     3241 cacatcggca cgacgccgag cttttcggcg cgcttcaaca cgcgcttcag cacctgcctg
     3301 ccgtcgagcg ggaacggtgc gtcgcggccg tccttccgga tcacaaattc gccgaggaag
     3361 aaatccacgc cgtcgtccca gggcaccttg cgatgcgtgc cgacgtcgat tcgcgcgagg
     3421 gcatcgggaa agcccttgtg ccacccggtg tacgtcgtgt tgtcgtagca ctggtcgtgg
     3481 caatcccagc cgtagacgac gtcgcagaag ccgaagcccg cctcgagcgc cgactcgaac
     3541 ttgtccttgt gcaggtactt gccgcgcagg atgccgtcga tgtccgacac ggccaccttc
     3601 acccggttgc cgggcgattt gcggaccgcg gcgaggatat cggcggcaac ggcggacgcg
     3661 ggcatcgcaa ttctccccgg caaagttgtt gtgtgcgtgc gtggctgtcg cggcgcgaag
     3721 acggcgacac cgccggcccc gtgcggcgtg ttccgattct agcagcccga agatgcgctc
     3781 attgcgcgcg gcggatgccg ccgagcgcgc ctgcgaaatc gcagaactgg ttctgcccga
     3841 cgatccgccc cgtgaatccg ctgatcgtcg gcgtcatctc cacgagcgcg aacgagccct
     3901 ggacgccgct cgtgcacgaa tagttgccgt tgacctggcc aagcttgccg gtttgcgaat
     3961 acgtcccgcc gaatgtgcag gtcgcgaccg atgtcgtggc cgtgatcgac atggcgctcc
     4021 cgttctgcgc gaccgccagg agggcggcca cttcctcgat gccgttcccg tttgcaggaa
     4081 agcagttcgt gttggtgatc gaatagccgc cgagatagtc gccgctgtag tcctcgttcg
     4141 tccacgtctg gccggcgaac caccggaaga ttcgacgtgc gtgcatcatg cgggctccgt
     4201 tgtgttggcg cgaggggcga cggttcgcgg gtcgcggggc gtcgatcgac ggcctgccca
     4261 ttcgcgcgtg gatcgtaccg aaggatgcca tccgcgcgca atttgacatc gccgccgtcc
     4321 tcctccacgc tcttcgcacc cggcccggat catgaaactg ctgctcgacc gctttgcgct
     4381 gttctacccg atgcggatcg tcgggacggc cttcatggtc atcgtcgcaa cgctgtacgc
     4441 atggcaccgc gatcggttcg gcgcgttcta ttacggcctc gtgctcgttc tcgtcgtcta
     4501 tccgcacgtg gtccaccatg tggcacgcaa gtacccgcag aatcggcgcg cgatcgagct
     4561 gcgaacgttc gtgttcgact cgttcgccgt cggcgtggtc gtgcactcga tcgggttcgg
     4621 tccatttccc acgttcgtgc tgatcacggt ggcgctggcg agcgcgcttt cggtggatgg
     4681 cccgcggcaa tcgttgctga gtgctgcggc cgtcgcggcc ggcatcgtcg cctacgccgt
     4741 gttctttccc gtcaatactg cgttcgtcga cacgatcgcc gtcgacatcg tgagctcggc
     4801 gttcacgttc gtctatttca tggcgtttgc ctattcggcc cgcaatcgca gcgcgctgct
     4861 gctgcgatcc gaggccgaat tgcgcgagcg caccgcgtcg ctcgaaatcg agaagcttcg
     4921 ctccgaccgg ttgctgttca atctgctccc atcgggattg gcagcgcaaa tggcgatgcc
     4981 gggtggcata cggccagcgc gtttcgagcg tgtcgtcctg ctcggcatcg agctgcggca
     5041 cttctcgcgc atgctgcaat cctcgcccga ggacgaggcg ctggcgcacc tgatgcactg
     5101 cttcaaggca ttcgacgcga tcgccgatcg cttcggcatg gaaaagctca agacgatggg
     5161 cgatgtctat atcgccatcg gcgggttgcc ggtgtcggcg ccgggtgacg tcgcggccgg
     5221 catccgcgcc gcgtcgagtg cccgcgagtt cctgcgcgac cttgccgagt cgcggcgcgc
     5281 ccacggcgcg ttcgtcctcg atgcacgaat cgccgtgcat gccggcagcg tgatcggcgg
     5341 catcgtcgag acgtcgaagc tgtcgtacga cgtgttcgga gacgcaatca ggacgctctt
     5401 cggcttgctg cgcggatgcg aggacggcgc gatcatcgtc tccgacgccg cgcggtgcct
     5461 ggcgggggct ggattcgaaa gacccgatat cgagacggcg ggcagcgcag ccgcatcgct
     5521 gtcccacgcc gacggcgcgc ggtcgcacgc gccatccgat gccgccacgc aggcgtagcg
     5581 ggtcgatgga cgacgaattc atccaggatc tgcgatcgct gctgccacgc tttctcggtc
     5641 cgttgcccga cccggagctc accgcattcg ccgctcgatt gcagcgcgag ctcgtcgcca
     5701 gcgggacgct cctgtaccgg caaggcgaag catcggactg catgcatttc gtcgtcaccg
     5761 ggcggctcga ggtctggacg catgaccgtt acggcgaccg gcgcctcgtt gcccatttgt
     5821 cgagcggcga ttgcgtcggc gagctttcgc ttttgaccgg cgacacgcgc gcggccgatg
     5881 tcgtcgtctt gcgcgatgcg gtgctcgccc gtctcggtcg cgacgattac gacgcgatgc
     5941 tgcgccggta tccggaagcg ggcctcaaca tcgcgcgatt cgcgctgcgc acgctgcgcc
     6001 agggcaccac cttcgtcccg aaggtgcgca acattgcgct cgtgcccctc ggaagcggcg
     6061 tgcgcgtcgc cgaattcggg cgccggctcg agctcgcgct gctgcgcttc ggcagcacgc
     6121 tgtatctcga ctcaccggtg gcccatgcgc gcctggcggc atcccgcgcc gggccggcac
     6181 acgcagcgac gagcgagcgg catctcgacc ggcttctcga cgaggcagag cacgcgcgcc
     6241 ggttcgtgat ttgcgaggcc gacgccgaat gcaccggatg gacgcgcaag tgcgtcgcac
     6301 atgccgatcg catcctgttc gtcgccgacg cagcgcaatc gcccgcgttg agcgcgatcg
     6361 aggaagcgct gagcggcgcc gcacccgggg catcgctcac gcaaagggat ctcgtcctgc
     6421 tgcacgcaaa ccgccgtgtt ccgcctgcgg gaacggcggc atggctcgcg ccgcgcaccg
     6481 gataccggca ttcgcacgtc gcgtgggagg gcaaccgcga tttccaccgg ctggcgcgac
     6541 tgctgtccgg caatgccgtg acgctggtgc ttggcggggg cggcgcacgc ggccttgcgc
     6601 agatcggcgt aatacgcgcg atccgcgagg cgggcgtccc gatcgatgcg gtgggcggca
     6661 ccagcgtcgg cgccatcatc ggcgcgctcg tcgccctcga ctggatggac gaggagatcc
     6721 tgcagtcgtg caagcacgcg ttcgtcgacg atcggccgct cgacgacgtc acggtgcccg
     6781 tgttctcgct gctttccgga aagaagttcg cgcgcacgct gcatcactac atcggcgacg
     6841 tcgacatcga ggatttgtgg cagccgtact tctgcgtgtc gagcaacctg tccgaaagcc
     6901 gcgtgcacgt gcatcatgcg ggcaggctgt gggaagcgat gcaggcgagt gccgcgttgc
     6961 cgggcatgtt gccgccgatg gtctccaacg gccagctcta cgtcgacggt ggcgtcctcg
     7021 acaacctgcc ggtcggggtg atgaagcgct tcatcggtgg caatacgatc gccgtcgagg
     7081 cgacggtcaa ggtcgaatat tcgatcgacg ccgccgaatt cccgtcgccg ctcgcctatc
     7141 tgcgctcgcg cctgtcgcgg cgcggccgat acgcgttgcc gacgctgccg agccttctga
     7201 tcaaatcgac gctgctgggc ggtggcagcg gacgcaacga gcttcgtgac ggcagcgacc
     7261 tgtacgtcaa tccaccgatg cgcgaattcg gattcctcga ctggcaccgg atctacacga
     7321 tggtcgacgt cggttaccgc catggacagc agtgcgtgcg tgcgtggctc gaggtccatc
     7381 ccgaattgca gcggcgcgac gagtcgccgg ggctgccgcg cacggccatg gcactatgag
     7441 gcagcgctcg aggtgccgat aggcgcgggc cgcacgtcgg cgctgcttgc gctgcacgtc
     7501 gcggtggcgc tgttcgggtt cgcggccctc ttcggcaaat ggatcgcgct ttctcctgtc
     7561 gccattgtcc tcggacgcac ggtcattgcc gcggcgacgc tcgcgctgct cgtctggatg
     7621 cggcaccggc cgctcggccg gcccgagcgc gcgctgctgg tgaacggtcc gctgcttgcg
     7681 ctgcactggg taagcttctt cgccgcagtg cagatcgcga gcgtcgcgat cgcgctgttc
     7741 ggctatgcaa gctttccggt gttcgtgctg ctgctcgagc ggggcaggcg cgcgagcggc
     7801 ggcgaatatg caaccgcgct gctcgccgcg ctgggtctcg caatgctagc cgcccatctt
     7861 tcatggtcgg gcgctggaat gcgcggtctt gcaatcgcgc tcgtctcggg cgtgacgttc
     7921 gcgctcctcg tcgtgcgcaa ccgccggctc gtcgccgggg gatcggccgc gcgcatcgcg
     7981 atgtggcaaa accttgccgc cggcgtctgc ctgctgccga tcgtcgcgct cgccgatcgc
     8041 ccggcggcgt ggccgcgatt cgcagacgcc gcactgctgc tcgtgctcgg cgtcgtttgc
     8101 acgggccttg cgcatacgct gttcattgcc agcatgcggc gcgtttcggc gcattcggcg
     8161 agcgtcgtgg cgtcgctcga accggtttac ggcattgcgc tggcggcatg gctgctgggc
     8221 gagaagccgg atgcgcgcac gatgggaggt ggcgtgctca tcgtggtcgc ggcgctcatt
     8281 gcgtcgcgcc gggttgaatg acggcagggc ggcgttggcg gtgacgccgg cgtttcggta
     8341 gcatcgcgtt ggaggatgaa atgaacccag gcatcgtcgg tctcggccgg agaatcgggc
     8401 aagcggtgag cgcgcgatga tcgtcgtgct catgggcgtt tccggttccg ggaagtcgac
     8461 cgtcggcgtc gccctcgctg cggcactcgg ctggcggctg atcgacgccg acgacctgca
     8521 ttcgccgcac aatgtcgcca agatggccgc gggcattccg ctcaccgacg acgaccgctg
     8581 gccatggctg gaccgcgtcg tcgacgagct tcgccgtgcc cgcgccgagg gcacgaacgt
     8641 ggtgctcgcc tgctcggcgt tgaagcagcg ctatcgcgac cggctcgcgc aggcgggcga
     8701 cgtgcgcttc gtctatctgc gcggcgatgc gcagacgatc gcggcgcggc tcgcgagccg
     8761 tcaccatcgc tacatgccgg cggcgttgct tgaaagccag ttcgcgacgc tcgaggcgcc
     8821 cagcgacgcg atcgacgtcg acatcacgca gagcgtcgac gcacaggttg ccacgatcat
     8881 cgcagcgctc tccgatggaa aggccccatg accggcggtt cgcgttatcg catcgccacc
     8941 gaggttgcgc atctcggacg cgatccgcgg cgtttcctgg gcgccgtcaa tacacccgtg
     9001 ttccgcgcgt cgaccatcct gtttcggacg gtcgccgagc tcgagcaggc ggcgcgcggc
     9061 gaattcccgg acctcgcata cgggctgcac ggcctgccga cggtcagcga cctcgcacgc
     9121 gcactggccg ccctcgaagg cggacatgca gcactcatcg tgccgtcggg gcttgccgcc
     9181 acgacgtttc cgctgctcgc gctcgcgaag cccggcgacc acgtgctggt gaccgatgcc
     9241 gtctacgggc cgacgcggcg tttctgtgac aaccacctgc gccggctcgg catcgacgtc
     9301 agctactacg atccgctgct cggtgccgaa attgcacgcg agttccggcc gcgcaccaca
     9361 ctcgtgttca ccgagtcgcc cggctcgctc acgttcgagg tgcaggacat cccggcgatc
     9421 gccgcggttg cgcatgcacg cggtgcgcgc gtgattctcg acaacacgtg ggcgacgcca
     9481 ctcgggttcc ggtcgttcga ccacggcgtc gacgtgtcgg tgcacgcggt gaccaagtac
     9541 atcggcggcc actccgatgt gctcctcggc gccgtgatcg ccagcgccga agcgtacccg
     9601 ccgcttgcgc ggctgtggac cgacatgggc atcacggcgt cgtccgacga ctgcttcctc
     9661 ggcctgcgcg gcctgcgcac gctggccgtg cgtcttgcac gccacgccgc gagcgcgatg
     9721 gaggttgcac gctggcttgc gcaacaaccc gaagtgcgcg aggtgctcta cccggcactg
     9781 ccggaggatc gcggccacgc gctgtgggcg cgcgacttcc atggcgcgtc gggactgttc
     9841 ggcgtggtgc tcgaaccggc cgatcgcgca cgcatcgcgg cgatgctcga cggcatgcgc
     9901 ctcttcggca tgggctggag ctggggtggc ttcgaaagcc tgatcattcc gacgtggccc
     9961 gagcgcgcgc gaacggcgac gtcctgggac gctggcggcc cgatgctgcg cctcgcgatc
    10021 ggcctcgaac acccgcagga cctgatcgac gatctcgacg acggattgcg ccggctgcgc
    10081 ggcgtttagg gcagcgttgc cacgccagag cgggcggcgg ccgcccgcag ctcacgatcg
    10141 aacgtcgcga gcgcgacgcg ccgtgattgc gcgagctgca ggtagcacgc gtcgtagatg
    10201 ctcaaggcat acgtgcggca aatttcactg agcgcgggca gaccggcggc cgtatgaggc
    10261 tggtcgagag aaacgggcag caccgcgaga tgctcgagaa attccttgcg attcgattcg
    10321 gagatccgct tgcgtcgctc cgccatgacc agcgcattgg caatctcgag ctgccagatc
    10381 gacggtgccc aagcgtcttc ctgctcgagc cgaacgagca gttcgcgcgc cgaggcgctc
    10441 gtctcgtctg gcaggaacca ggcgagcgca atggacgcgt cgagcaccag ccccatcagt
    10501 agtggtgccc ttcatgaagg ccgtcgcgaa tgcgctccgg attgcccagc gcgtcgcgca
    10561 gcgtaacgcc gcgtttcgcc agtcgattcc ggagccggtc gattgcggtc acggcggcgt
    10621 ggatcgtctg cgggtcccgc ctggcgaccg ggccgagctc ggcgacaggt cgtccgtgcc
    10681 gggtgatgac gaagcgctca cctcgttcga cgcgctcgag caaggccggc aggtgcgtct
    10741 tggcctcgta ggatccgatc tcgttcattt ttgaagtctc cggcagtaga ctagtctaga
    10801 ccggtctgaa aaaatcgtca aggcgcgaag cacggcacag gcgcgaagcc ggcactcgcg
    10861 cagcgcctac agcgagctcg cgacgtggcc gccgtcgacg gtgacgacgc tgccggtcat
    10921 gtaggaaccc gcgtccgagg cgagcagcag aagcgcgccg tcgagctcgt cgggcatgcc
    10981 gatgcgccgc tgcgggacgc gcttgatcat tgcctgcccg gcgtcgctcg cgaaaaagtc
    11041 gtggttcatc gccgtctcga catagccggg tgcgagcgcg ttgacgcgaa ttcgatggcg
    11101 tgcgagctcg agcgccatcg ccttcgtcaa ctggatcagc gccgccttcg acgctgcgta
    11161 gctcggcacc tgtgccgcga cgcgcaggcc aaggatcgac gcgacgttga cgatgctgcc
    11221 accggcgccg tgcgcgacca tgtggcgtgc cgccgcctgc gcgacgcgcc acgcgccgtt
    11281 gaggttgacg tcgatgacgc gcagccagtc gtcctcggtg tggtcgagca acggcttggt
    11341 gatcgcgatg cccgcgttgt tgacgacgac agtgattgca ccaagcgcgt gcgcggcatg
    11401 gtcgaacgtc cgcgcgacac tggcggcgtc ggtgacgtcg agcgcgagcg gtacggcacg
    11461 ccggccgagc ttcgcgattt cgtcgacgag cggatcgagc gcatcgacgc cacgcgccgt
    11521 catcgtgacg ttcgcgcccg ctttggccag cgttcgcgcg aagtggccgc cgagactgcc
    11581 gttggcgccg gtgacgaggg cgtggcgtcc tgcgagcgag agatccacgt cactcctttc
    11641 gtttcagcgc aacgccttgg ggttcgagat cgcgagccgc tgcacggcga tttcgtcgag
    11701 cgcggcgagg agcgtcttcg catgcacgtc gtcgtcgaag cggccatcgc ggatcgcggc
    11761 cgcaacccta cgccgcagcg cctgcacgtc gtcgctgccg gcatcgctcg gcggtaccgg
    11821 ctgtgccgcg agcggccgca gcagctcgat ctcgcgacgg atcgcatcgt ggccactcgc
    11881 catttcgcgt gcagcgatgg cgagcgcgtt cgcgaccatc agcccgcgat agcgcagctc
    11941 tccggagagg gcgggcagca actcgcttgc gagcgtctcg cgtgccgttg cgagaagcgc
    12001 ggccgcgtcc ggcacgtcgt tgcgacggct tgatccggag gtggactgcg gattcgcttc
    12061 ccgagtcatt ttctgcgttg cgtcaattgc agcacctcgt attcgagttc gtcgacgatg
    12121 cgcccggtca gcgcgagctc gagcgaacgc tcgtcgcgat ggcgctgcgc ctgctgcagc
    12181 gcgatgagcg cccagcgcag atgcgccatc gtctgccagt aatccagatc gtcgcgcgag
    12241 atgccgtggc cggcaaccga tgcatagccc gcgatgaacg gctccagcgc cgcgatgccg
    12301 cccgcttcga gatcgcgtcg cgcgaagcgc cagcagcgcg ccgtgaacca gccgaggtcc
    12361 tcgcgcggat ctccccatcc cgcgaactcc cagtcgagca cgccggcgag cttgccgccg
    12421 tcgacgagat aattgccggt ccggtaatcg cgatgcacga acgtgacctc gtacgtcgac
    12481 ggcgcgtgcc gctcgcacca gcgcaggccc cattcgagga tcggatacgc gccgtcgagc
    12541 cggtcgagcc aggcgcgata ggtcgcgatg ccttcgagcg ccggattgcc cgaactgcct
    12601 cttccgttcg tagcggaaga cctgggcgac ggccggatcg catgaagttt ggcgagattg
    12661 gcaccgagct catacgcgag cgccggcgga tcgggaacga gtgccgcgtc gcgcgtgatg
    12721 cgatgcccgg ccgcgacacc ggacaggcgc tccatgacga agaactcgct gccgagcacg
    12781 gcaggatcgg tgcagagaaa cagcggcttc ggcgcatgca cgccggccgc gtatgcagtg
    12841 cgcagcacct cgtactccga cgcccggctt gcgctggcat cgacgcgtga gcgggcgttc
    12901 gtgcgcagga cccacgactg cctgccctgc cagccgccgc cccggatatc gacatcgagc
    12961 aggtagttgt gctggatcgc acctccgcgc aggcgctcga agcgtgtcac gcgcacgtcg
    13021 tctgcacccg tctccgcgcg cagccacgcg gccagtgtgt cggctcccgc aacgtcgctt
    13081 gccgtcacgt cgttcaatgc gctgcgggtg ggccgccggc gcgacgcggc gcgtcgcgca
    13141 gaatccgccg cgcgatactg tagcggtgaa cctccgacgg cccgtcgtaa atccggaacg
    13201 cccgcaggtc ggcgaagatt cgtgcaacca ccgtttcgtt cgtcacgccc tgtccgccga
    13261 ggacctgcac gcagcggtcg gcaacacgcc atgccgcttc cgatacgatc accttggcgc
    13321 ggctcgactc gtgcaacgcg agctcgccgc gatcgaggag ccacgcgcag tgccagatcg
    13381 ttagacgcgc ggcgtgcatg tccatttcgt tgtcggcgag catgaaaccg acaccttcat
    13441 ggtcgccgat gcgcttgccg aacgcgtgcc tgcgcttcgc gtaatccacc gcaacgtcgt
    13501 gcgcgcgccg cacggcgccg agccagcgca tgcagtgcgt gagtcgcgcg ggcgacaaac
    13561 gcacctgcgc gtagcgaaac cccttgccaa cttcgccgag caccgcgtcg gccggcacgc
    13621 gcaggccgtc gaagcgcacg accgcgtgcc cgcccggaaa gcattcgtcg agcgcatcca
    13681 tggcgcgttc gacgatgatg cccgccgtat tcatgtcgca caggaacatc gtcgcaccgg
    13741 cgtcgtcggc cttcgccatg atgatcgcga aggccgcacc gtcggccccg gtgatgaacc
    13801 acttggcgcc gttgatcacg tagtcgccgc cgtcgcgcgt ggcggtcgac atcagcatcg
    13861 acgggtcgga ccccgcaccc ggtgccggct ccgtcatgca gaagcacgag cggatcgtgc
    13921 cggcggcgag ggggcgcagc cagcgctcct tctgggcggg cgtggcgacg gcctcgagca
    13981 gatgcatgtt gccttcatcg ggcgcaaaca cattgagcgc gaccggtccg agtggcgagt
    14041 agccggcctc ctcgaacgcg atggcgcgtc ccacatgcgt gagcgcgagg ccgccgtatt
    14101 ccttcgccac gtgcggcgcg aggaggccgg cttcgcgtgc cttgtcgatg agctcgcggc
    14161 gcaattcctc gctcggcccg tggcgcgtgc gtcgcgcgtc cgattcgagc ggaatgatct
    14221 cgtcggcgat gaagcggcga atgcgcgcct gcagcccggc aagctcgggt gggatttcga
    14281 aatcgatcgt catgtcagtc gtgaagggcc gccccgaacg actgacccgc ccccccgggg
    14341 ggcagcgagc gcagcgagcg tgggggccgt gtcatcctag ctcctgcccg atgcgtcggc
    14401 gccgcttcgc agccgttcgc gcaatgccga cttccggacc ttccccgtcg acgttttcgg
    14461 cagcacgccg aacacgacgc gcttcggcag cttgaagcgt gcaagacgcc cccgcgcgtg
    14521 ctcgatcaat tcgtcgccgc tcgcgtcttc accttccttg agcgtcacga aagcaaccgg
    14581 cacttcgccc cacttgtcgt cgggtgccgc gacgacggcc acttcgagca ccgcgggatg
    14641 ctgcgtcagc gcctgctcga cctcgacgga cgagatgttt tcgccgcccg aaatgatgat
    14701 gtccttcgcc cggtcgcgga gctcgacgta gccgtcgggg tgaagcacgc cgaggtcgcc
    14761 cgtgcgaaac cagccgtccg ggcacgcctt gcgcgtcgcc gcgtcgtcct cgtaatagcc
    14821 gagcatcacg ttgttgccgc gaatggcgat ctcgcccatt gtcgtcgcat cggccggcac
    14881 gtcgcgaccg tctgcgtcga cgacacgaat cgtctcgccg atcgcgttcg ccactccctg
    14941 gcgagccttg agccgcgcct gctgctcgat gcgcaggccg ttccattcgc tgcgccattc
    15001 gcagatgccc gcgggtccgt aggtttcagt caacccgtac agatgcgtga cgtcgaggcc
    15061 gagcgcggcc atccgttcga gtagcgccgg tgttggcggc gcaccaccgg tagcgatgcg
    15121 cgccacacgt tccaccggag ctgcggcggc atcccaggcg atcatcgtga gcaccgtagg
    15181 cgcgccgcaa aggtgcgtga cgcccgattc gcgcaggtgc cgccagatcg tcgccggctc
    15241 gagcttgcgc aggcacagat ggacgccgcc catcgcggtc accgcccagg gaaagcacca
    15301 gccgttgcaa tggaacatcg gcaacgtcca aagatagacg gaatcgctcg tgatccccat
    15361 ctgcatcgcc atcgccagcg cctgcagata ggcgccacga tggtgataca tcacgccctt
    15421 gggccgtgcc gtcgtgccgc tcgtgtagtt gatcgcaagc agtccgcgct cgtcggcgac
    15481 cttctgttcc aaggcttcgg cattggcgat cgcgagctcg agctcgtcgt tgctgccgcc
    15541 ggcgcggagc gacctgatgt cgattgcggc ttcggcgagc gcctgctcgg ccgtggaacg
    15601 gaagtcgtcg tcgtagatca gtaccgagac gcccgcgtga cgaaggatgt acgcgatgtc
    15661 ggccggcgtg atgcgcgtat tgagcgcgac gagcacggca cccgcgaacg gaaccgcgta
    15721 atgcgcggcg agcagcacgt gcgtgttcgg cgccagcacc gcgacgcgat cgcccgccgc
    15781 gacgcccatg gcgcgcagaa ccccggccat gcgccggcat cgctcgtaaa acgcgacgta
    15841 cgtgaagcgc gtgtcggcgt cgacgatgcc gacgcgatcg ccgaagacgt aggcggcgcg
    15901 gcgcaggaac gctgtcggcg tcagcgattc gtaattgaat tcaagtgcca tctccttgct
    15961 ccattcacgt tcccagatac gcggcctgca cgtcgcgatt ctcgcgtatt gcctgcggtg
    16021 ttccctcggc aatgatttcg ccgcggtcga ggacgataat gcgctcggcg aagccgaaca
    16081 cgatgtccat gtcgtgctcg gtgaacagga cggtcctgcc gtcggcacgc gcgagatcga
    16141 cgacgagctg catcaggtcg cgccgcgagc gtggcgtcat gccggccgtc ggctcgtcca
    16201 tcagcaacaa tcccggcgca ccggcaagcg ccatcgcgag ctcgacccgt ttggcatcgc
    16261 cgtacgcaag ccctgcgcaa tgggcgtcgc gaagatcgcc gatgcgcagc cgggcgagca
    16321 acgcgtcggc cggggccgac acggccggca gcaacctcga catcaccgcg gcgtcgcgtt
    16381 cggcgtgcgc cgcgagtgcc agtgccatgc tctcgcgcac cgtcatcgac gcgaacatcg
    16441 cggccacctg gaacgtgcgc ccgatgcctg cacgcgcgat cgcgtgcgtg gcctggccga
    16501 ccagcgagcg cccgtcgaac accacgtctc cgcggtccga aacgagctcg ccgtggatca
    16561 ggttgaaaag cgtcgtcttg ccggcgccgt tcgggccgat cattgccacg acctcgccgg
    16621 cggccacgga gaaggacacg ttgcgcaccg catcgacgcc gtcgaacgac ttgctgaccg
    16681 ccgacacctc gagcagcggc gcgatcatcg ggccgggcgc gtccgcaggc gaagcagccc
    16741 accgccgatg ccgttgggaa acacgctgac gatcagcagg atcgccacgc cgacgcatgc
    16801 acgccagtat tcggtgtagc ggcccagcac gtcggaaagc cacgtgaatg ccgtcgcgcc
    16861 gagcagcggc ccgaagagcg cgttctggcc gcccagcagc agcatcacga gcgcgtcgac
    16921 cgagcgcggg atcgacagcg catcgggcgc gacgccgccc ttcgagaacg cgaagagcgc
    16981 gcccgcgagc cccgcaaacg cgccggcgag cacgaggccc ttcatgcgcc gtgcgcgaac
    17041 gtcgatcccg agcgccgcgg cacgcagcgg cgaatctcgc gacccgcgca gcgcgtagcc
    17101 gaacggcgtg aaggcaatcc ccgccaatgc gacgagtgcc gcgccggcga cgatcagcac
    17161 gaacaggaaa tagcgtgtcc gctcgccgag ccactccggc ggccacgcgc cggtgattcc
    17221 gttcgagccg ccggtgagcg tatcccactg aatggcaacc gaccacagaa tctgcgcgaa
    17281 ggcgagcgtg agcatcgcga gatagatgcc cgacatgcgc acggccagcg cgccgaatac
    17341 gaatgccgca agggctgcca ccatcggcgc agcgacgagc gcaagcggga agggccaccc
    17401 gtgcgtcgcg gccaggcagg tcgcgtaggc cccgacgccg aagtacgcgg cgtgcccgaa
    17461 cgacgtcatg ccgccgcgcg caacgatcag ctgcaggctg gccgtgaaaa gtgccgcgat
    17521 gaaaatgtcg gtggcgagta ccagcagata cgcatcgcgc gcgagcggca gcagcaatgc
    17581 ggcgacgacg agcaccgccg cgaccatcat gctgcgccgg tgcggtcgct gcacgagcgc
    17641 gcgctcggcc acgacggccg tcgtcgccgg cacgctggcc ggcgtgccga agagccccgc
    17701 gggccggacc agcaggacga gtgccatcag cacgaactcg atgaccagcg tgagcttcgg
    17761 caacgcgacg gcaacgtcgc cgacgtgaat cgtccccacc gcgatgcaca tcgccttcgt
    17821 gacgccgatg atgaccgcgg cgatgaaggc gcccggaatc gagcccaatc ccccgaggac
    17881 cgtcacgacg aacgcatcgg cgatcacgcc gagatccatg ccgagatgcg ccggttcgcg
    17941 tggcagcgca agcgcgccgg cgagtccggc gagaaacgat ccgagcgcga ataccgcggt
    18001 gaacagcgtg cgctcgtcga cgccaagggc agcggcgagc acgcgatgct cggaggcggc
    18061 gcgcacgagc atgccgaagc gcgttcgccg cacgatccac gtgagcgcga tcagcacgag
    18121 cggaccgacg acgagcagca acaaatcgta ttcggggatc gcctgtccga gccagggaag
    18181 cgcaccgtcg aggcccggaa cgcgcgggcc gagccggtcc tccggccccc acaatgcaag
    18241 cgccgcatcg ccggcgatca gcacgattgc gaacgtcgcc gtgagttgca gaagctcggg
    18301 cgccggatag agccgcttca ggagcagcac ctcgatcagc gcgccgagca gcgcgaccgc
    18361 gagtgccgcg acgagcgatg ccgcccagaa gccgagcggc gagcccgcgt aggcaccgaa
    18421 tgccgtcgcc gtcgaataag cgacgtacgc gccgagcatg aagagcgagc cgtgggcgaa
    18481 gttgacgatc cgcgtgacgc cgaagatcag cgtgaggccg gcagcgagca ggaagagcga
    18541 ggaggcggac gcgaggccgt tcaggatctg gacagcgagc gacgcgaacg acacgtaggg
    18601 gccagtcagg tgcgcggggc cgccccaagc gaccgactgc acccccttgg gggcagcgag
    18661 cgtgggggtg gcatcactac tgagcgggcc gcagccgccg caccacgtcg tcgggcggca
    18721 ggtagtcctt gccgtctgcg taccgccagt cgaccatgat gcccttgccg tcgcgcacgt
    18781 ccgtgcgccc gacatacgcg cccatcgtcg actgatggtc gattgcgcgg tacgtgacct
    18841 ggccgaacgg tgtttccagc gtgagattct gcatcgcggc gacgagcttc tcggtgtcgg
    18901 tcgagccggc gcgcgcgagc gccgccgccg cggacttcac ggcgctgtaa ccgacgaccg
    18961 acccgagccg cgggtagtcg ttgaacttcg cctggtaggc gtcgcgaaag cgcttgtgct
    19021 cgggcgtgtc gatcgcgtac cacggatagc cggttacata ccagccgatc ggcgcctcgt
    19081 ccttcagcgg atcgaggtac tcgggctcgc cgcccagcag gttgaacacg ggccgaccat
    19141 tgaagagacc ccgcagccgt ccctcgcgaa cgaagcgcgc gagatcgggg ccgaagagcg
    19201 acgagaagat cgcatcgggc gcagcgtcga tcagcgcctg caccacggcc ccgggctcga
    19261 tcttgccgag cggcaccgcc tgctcgacga actcgagtcc cggaccctgc tgcgcctgca
    19321 tttgcgcctt gaacgtcgcc gtcgccgcct ggccgtactc gtagttcgga tacacgatcg
    19381 cccagcgctt tttggcgagc ttcaccgcgc cggggacgag catcgccgtc tgcatgtagg
    19441 tcgatgcgcg cagcctgaac gtgtacttgt tgccgtcctc ccacacgatc ttgtcggtca
    19501 gcggctcggc cgcgatgaag agcacgtggc gccgcttcgc gagatcggcg atcgcaagcc
    19561 cgatgttcga ggcgaaggtc ccggtcagca acgcgacgtg ctcgcgcgtc agcagctcct
    19621 cggcgacgcg cacggcgtcg ccgggaacgc cgttgtcgtc gcgcgacacg acttcgatct
    19681 ttttgccgag aacgccgccg gcgccattga tttcatcgac tgcaagctcc atacctttac
    19741 gatagggctc gagaaacgcc ggaaactgct tgtactcgtt gatctcaccg accttgatcg
    19801 tctgcgcgac cgcgttcggc gcgctcgacg cccatgcgga cagcacaatc agcagcagac
    19861 tcgtcaagcg gtgtgccatt cggactcccc gggatggacc aggctggggc cacgggcgac
    19921 gcggcaaccg atggtagcaa acggcggctc gaccgaccgg aaacgataaa atgcgggatt
    19981 tgcctgcggc ggacttctcc gcgccgcgca accgccgtca ccccatcatg cttcgactag
    20041 cccacggcct cgagttcgcc gacctctata cggtcgacgg cgcggcgcgc atcgacgagc
    20101 gcttcattgc gcatcttgcg cgtgccgacg ccaccgtggc cgagcgcctc cttgaggcac
    20161 ggaccgcgcc cgcgccactc gccggcaagg acgaatcggc gctgctgatc gacatcgcac
    20221 cgcatctcga ggatttcctg gccgagctct tcggcatcga ggacgctgtg cgcgcactcg
    20281 aggcgcgtca ccacgagctc gcgccgctat tttccgtgaa gcgccagttc gtgcagcgca
    20341 aggcgatgaa cgcgtacaag gccgacgtcg ccgcgtcctt cgatggccgt gcgcttcgcg
    20401 gcgcaatcga cgcgttgctc ggtgcgcacg gcgacgtagc ctcgttcgag ctcgcgttcg
    20461 cacgtgcggt catttcatgg cagcaggacg aagcggcgaa cgcgacggcg ctcgacgtcg
    20521 cgctgcgcta tgcggcgtgg gccgcccatg cgcaggtagg caggcgggcg catcgtggcg
    20581 gcgtgctctt tcgcgcgccg cgcaagctcg acgcgcttcg gctcgttccg gtcgagtcgg
    20641 tcgtgcagga tggcgtggcg atgctccagc atccgcacgc cgccggattg cgtcgccgcg
    20701 cgggtttcac gctcaccgac gcgggaaccg atctcacggg cggcctcgac caggcgcatt
    20761 actgcatctg gtgccacgaa cagggcaagg actcgtgcgc gcatgggttg cccgaaaagc
    20821 gagcgcgacc cgccgccatc gatgcccccg atgaggcagc cgcccatccg tacaagcaat
    20881 cggtctttgg cgtgacgctc gccggctgtc cgctcgagga gcgcatatcc gagttccaca
    20941 agctgcgtgc cgaagggtgg gcgcttggcg cgcttgccac gatctgcgtc gacaatccga
    21001 tggtagccgc caccggccac cggatctgca acgactgcat gaaggcgtgc atctaccaga
    21061 agcaggatcc ggtcgacatc cctcaatcgg aaacgcgcgt gctgaaggac gtactcgcgt
    21121 tgccgtacgg cttcgagatc tattcgctgc tgacgcgctg gaatccgctt gacctgcgcc
    21181 gtccgaagcc gctgcaggcg acgggcaagc gcgtgctggt cgtcgggcag ggtcccgcgg
    21241 gattcacgct cgcgcaccac ctgctgaacg agggccacac ggtcgtcgcc atcgacggcc
    21301 tcaagatcga gccgctcgat cccgcgctgt ccggcgtgcg tccttcgggc gagcgcgtgc
    21361 cgttcatgcc ggtcgcggat ttcgcgtcgc tgaccgagcc gctcgacgat cgcgtgatgg
    21421 cgggtttcgg cggcgttgcc gaatacggca tcaccgtgcg ctgggacaag aacttcctca
    21481 aggtgatccg cctgttgctg gagcggcgtt cgaactatgc gctgtacggc ggcgtgcgtt
    21541 tcggcggcac ggtcgacgcc gacgacgcgt tcgcgatggg attcgaccat atcgcgctgg
    21601 cggcgggcgc cggccggccg acggtcctcg acctgccgca tggccttgcg cacggcgttc
    21661 gcaccgcgtc cgacttcctg atggcgctgc aactgaccgg tgcggcaaag gacgattcga
    21721 tcgccaacat gcaggtgcgc ctgccggtgg tcgtcatcgg tggcggcctc accgccatcg
    21781 ataccgcaac ggaggcgctc gcgtattacc cggtgcaggt cgagaaattt ctcgcgcgct
    21841 acgaggcgct cgtcgcactc ggcggccgcg gcggcgtggc agcggcgtgg agcgagcacg
    21901 aggcgtcgat cgccgctgaa ttcatcgccc atgcgcacgc aatccgcgcc gaacgcgcgc
    21961 aggccgcggc cgagcatcgc gaacccgcac tcgccgaact gttgcgcgcg tggggcggcg
    22021 caacgatcgc ctatcgcaag cgcctgatcg acagcccgtc gtacacgctt aatcacgagg
    22081 aagtcgacaa ggcgctggag gagggcatcc gcttcgccga gggcctgacg ccgctcgcga
    22141 tcgacgtgga cgacgccggt cacgccatgg cgcttcgcgt gtcggtgcag acgcggaccg
    22201 acgagggcgt atggatcgag caggcgcggg cgcggatgcc tgcgcgcacg atcctcgtcg
    22261 ccgcgggcac gcagccgaac accgtgctcg cacgcgagga cgcgacgcat ttcacgctcg
    22321 acgggcgcta tttccgtttg ctgcacgagg acggctcgct cgcgcaaccg gtacgcggcc
    22381 tcgccaagcc ggcgcagccg gcggttctga cgcacgtcgg tgacgacggc cgcgcaatca
    22441 gcttcttcgg cgacctgcat ccatcgttcc acggcaatgt cgtcaaggcg atggcgagtg
    22501 cgaagcaggg ctacccgatc gtgtcgcggt tgctcgcgcg cacggattcg gtattgcgcc
    22561 gccccgacac cgagttcttt gcgatgctga acgatgcatt tcgcgcccgc gtccagcgcg
    22621 tcgtgcgcct tactccgacg atcgtcgagg tcgtcgtgca tgcgccggcc gccgcgcgca
    22681 agttccggcc gggccagttc taccggctgc agaatttcga gtcgctcgcg cagcgcaacg
    22741 aaggcacgcg gctcgcgatg gaaggtctcg cgctcaccgg ggcgtgggtg gaccgcgagc
    22801 gcgggctcgt ctcggtgatc gtgctggaga tgggcggctc gtcggacctt tgcgtggcgc
    22861 tcgagcccgg cgagccgatc gtgctcatgg gtccgaccgg tgcgccgacc gaaatcgagc
    22921 acgacaagac cgtcgtgctg gtgggcggcg gtcttggcaa cgcggtgctg ttctccatcg
    22981 gccgcgcgtt ccgcgcagcg ggatcgaagg tgctttattt cgccggctac cggcgcatcg
    23041 aggatcgcta caaggtcgac gagatcgagg cagcggccga cgccgtcgtc tggtgctgcg
    23101 acgaggcacc gggcttcgcg gcgacgcggc cgcaggatcg cgtgttcacc ggcaatatcg
    23161 tcgagggcat gcgcgcgtac gcggccggag aactgggaga gcaaccgatt gcgttcggcg
    23221 actgcgaccg catcgtcgcc atcggctccg acaagatgat ggcggcggtc gccgcggcgc
    23281 gccacgacgc gcttgcgccg tacctttcgc cgcggcaccg ggcgatcggg tcgatcaact
    23341 cgccgatgca atgcatgatg aaggagatct gcgcgcagtg cctgcagccg cagcgcgatc
    23401 cggtgagcgg gcgcacacgt tacgtgttct cctgcttcaa ccaggaccag ccgctcgacg
    23461 acgtcgattt tccggcgctc gacgcgcgtc tgcggcagaa cgccgtgcag gaaaagctga
    23521 cggcgcgctg ggtcgcgcgc gtcaacgccg ccgccgaaag cgactgagcg cgacgttgct
    23581 cgacgccgcg tgtcgacgcc tcgcctgcgc gctccggtac gtgatcagtg ttgcatcgtg
    23641 gcgtgcgccg cggccatccg cgagctgaag aaaaggaaga aattcgagcc cacctcgacc
    23701 gcgcggccag ccgcttcggc ggcgtccatc gcggtcgagg acgtgatgcc gctgctgccg
    23761 tcggccgtcg gccagtcgcg agggtccatt accagcaccg gccgcacctg ccaccagatg
    23821 gggcgcgcgt tgacgcggga attgtcgacg acgtggctgt ggttgggcgt cggcaggaac
    23881 accggtgccg tcggcggatc gggcgccttg ccgagcgcac cgagaagcac ggacaggtcg
    23941 acggaggacg cgtgcatcgt gcaggtgccc gcgaccggat ggttcggatc gggacactgc
    24001 gtggtaactg cgggcgtggt cttccacgct tcgggtacga agccgaagag cgagatcagc
    24061 gcgctgccga gcgcggttcc gcagagctcg ttcgggcgtc cgccatcggg acacgccatg
    24121 gcatcttcag gatgggtatc gctgccgaac atcggcacga gcacgtagag atgcgcggta
    24181 tgcgcgatat cgccgccgga cggatcgctc gacggctcgg tgatcgcctg gcagatcggc
    24241 gtctgcatct cgttcggatc ggacgcggcc gcgatgccgt tgaagtcgag gtcgagttgc
    24301 ggctcgtcga cgcagtcgaa gttctgcaga taggtgaagg tgacgaggcc gccgttgccg
    24361 aagccctgcg tctggttggg agcgagctgt gcggacgcag ggttggcggc aaacacgatg
    24421 caagcgcccg cggccagcgg cagcgcccgg tgagccgcgc gcaggatcga gcgcgcgacg
    24481 gatgcggatt tgcgcatgga aactcctcgt tgcaatggat gacggaaaag cagcgcgatg
    24541 cagcgatcgc gccggccttg tcgcgccggc aatccgcagg tgatcgcggt gcgggcgtta
    24601 ccgcaaacag gcgaacgcaa tgcgcattcc acgacgtgcg aacggccgcg gatctgcgtt
    24661 cggttc
//
LOCUS       DASSVX010000043         4156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_568178, whole genome shotgun sequence.
ACCESSION   DASSVX010000043 DASSVX010000000
VERSION     DASSVX010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568178"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFO53_02085"
     CDS             complement(<1..521)
                     /locus_tag="VFO53_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET8875092.1"
                     /translation="MTTAAPKNAAAATRTAGPSQGASSTPRGAAQRRTPQAPGDHLEI
                     NGRRYRWPGRPLVVVCVDGCEPDYVNQAITAGSAPWMAGLNGRGTCLTADCVVPSFTN
                     PNNLSIVTGAPPSVHGICGNYFFDPDAREEVMMNDPKYLRAGTILAAFADAGAAVAVI
                     TAKDKLRALLGHRL"
     gene            complement(518..1273)
                     /locus_tag="VFO53_02090"
     CDS             complement(518..1273)
                     /locus_tag="VFO53_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate utilization associated
                     transcriptional regulator"
                     /protein_id="HET8875093.1"
                     /translation="MLKAPAPIPAVPGAVRGRTVGDDHPIALIQMNSLPGLVQRELER
                     MILAGDLPAGSKLRETEVASMLGVSRGPVREAFRALEESGLVRLEKNRGVFVRQIGID
                     EADQIFELRAVLDEFAGRRAAQNATPAQATELRGLVGQMEKAVARGDVDGYHDANVAF
                     HDRIVELAGNAKLTFVYRRLVNELHLHRRAALGQSGILPLSMREHRAIVDHIAARQPA
                     AAGRALHEHAMASRERVHHTSGVPTQRTGKSAR"
     gene            1479..2489
                     /locus_tag="VFO53_02095"
     CDS             1479..2489
                     /locus_tag="VFO53_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HET8875094.1"
                     /translation="MLAFALAAFAGAALAQSNVVTIYAADGLHDGNPSWFGDQFAAFT
                     KATGIKVQYIEAGSSGVADRIAKEKSNTQADVLVTLPPFMQRAAADGLLQPYTPAGAD
                     KLAERDKDPKGLYVAMMNNYPNFIYNASALKDAPKTYEDLLDPKFRKKLQYSTPGQAG
                     DGTALMLQAFHAFGSKEAGLAYLGKLQANNLGPSASTGKLTALVNKGEIYVANGDLQM
                     NLAQMRNNPNVQVFFPAGPNGKRSVFALPYFIGLVAGAPHADNGKKLIDFLMSQEAQS
                     TISSVAQGFPVRSDVHPSDENFKKLNAMLEGVEIWSPDWSKVLSDLQADVAAYNKAIS
                     GN"
     gene            2486..3646
                     /locus_tag="VFO53_02100"
     CDS             2486..3646
                     /locus_tag="VFO53_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875095.1"
                     /translation="MMPADFAGAAVAVPASTRAGAKRAGASSSGIGFEDVSVAYNGVV
                     VLDALTLAVNPGEIVALIGPSGSGKTTALRAVAGFVRPTRGRITIGTRDVTNVAPHAR
                     NIGMVVQNYALFPHMRAEENVAFGLRARGAGADVIRERVPECLRLVGMASFAARYPRE
                     LSGGQQQRVAIARALAIRPQVLLLDEPLSALDAQIRRSMLDELAKLHRELPSLTVLYV
                     THDQTEALTLADHIGIMRDGKLVAFGDAQSLYRYPPNRFSAEFLGRANLLPVQALAGA
                     GAGMARVRFGEGALLARNHHGLPDGGHCLLCVRPHDVSLAPPEKDANALAATVRSVQW
                     QGDMQNVELDVGGHVLRLSCPPMHEPPPPGAALTVHFSATQATLVPETMARG"
     gene            3639..>4156
                     /locus_tag="VFO53_02105"
     CDS             3639..>4156
                     /locus_tag="VFO53_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896948.1"
                     /note="involved in the transport of
                     2-aminoethylphosphonate into the cell; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate ABC transporter
                     permease"
                     /protein_id="HET8875096.1"
                     /translation="MADLALGAPYAAQPRTSSFWIAPPLLILAALFFFPLWLILRQAL
                     AGDGDVLSLAKFVGVIASGEFRHALVNTIVIALSATAGCLVLGFVLSVLIAFTPFPGS
                     EVLARMIDTFIALPTFLVALAFTFIYGSAGMLNESLKVLLHLELPPVDFLYSQWGVIL
                     SEVTVYTPFVMRP"
BASE COUNT          643 a         1422 c         1401 g          690 t
ORIGIN      
        1 agccgatggc cgaggagggc gcgcagcttg tccttcgcag tgatcacggc gaccgcagcc
       61 cctgcatcgg cgaacgctgc gaggatggtg cccgcccgaa gatatttcgg gtcgttcatc
      121 atcacttcct cgcgcgcatc ggggtcgaag aaatagttgc cgcagatacc gtgcaccgac
      181 ggcggtgcac cggtgacgat cgacaggttg ttcggattgg tgaacgacgg cacgacgcag
      241 tcggcggtga ggcaggtgcc gcggccgttc agcccggcca tccacggcgc gctgccggcg
      301 gtgatggcct gattcacgta atcgggctcg cagccgtcga cgcacacgac gacgagcggg
      361 cgcccgggcc agcggtaacg ccggccgttg atctcgaggt gatcccccgg cgcttgcggc
      421 gttcgccgct gcgctgcccc ccggggggtg gaactcgcac cttgggacgg ccccgcggtg
      481 cgcgtggctg ctgcggcgtt cttcggtgct gctgtcgtca tcgtgcggat tttcccgttc
      541 gttgggtggg cacgccgctg gtgtggtgaa cgcgctcgcg gctcgccatc gcgtgctcgt
      601 gcagcgcgcg cccggccgcg gccggctggc gcgccgcgat gtggtcgacg atcgcccgat
      661 gctcgcgcat cgacagcggc aggatgccgc tctgcccgag cgcggcgcgc cgatgcaggt
      721 gcaactcgtt gacgaggcgc cgatagacga acgtgagctt cgcattgccg gcaagctcga
      781 cgatgcgatc gtggaaggcg acgttcgcgt cgtggtaacc gtcgacgtcg ccacgcgcca
      841 ccgccttttc catctgcccg acgaggcccc gcaactcggt ggcctgcgcc ggcgtcgcgt
      901 tctgcgcggc gcgccggccc gcgaattcgt cgagcaccgc gcgcagctcg aagatctggt
      961 cggcttcgtc gatgccgatc tggcgcacga acacgccgcg attcttttcg agccgcacga
     1021 gcccggattc ctcgagcgcg cgaaacgcct cgcgcaccgg gccgcgcgac acgccgagca
     1081 tcgacgccac ctcggtctcg cgcagcttcg atcctgccgg aaggtcgccg gcgagaatca
     1141 tccgctcgag ctcgcgctgc acgagtcccg gcagcgaatt catctggatc agcgcgatcg
     1201 ggtgatcgtc gccgaccgtg cggccccgga cggcgcccgg gacggcgggg atcggggcgg
     1261 gcgccttcag catgttgcca ttaaaatctg ttggctgtag attgtcaaca atccggcgcg
     1321 cgcaccgcag gcatcgcgcc ccggcagcga cggccacacg cgagataaga ttgcggccgg
     1381 cgcattcctg tccgttgcat ccgcaaggtc gcgcgggcat gatcaacgac gttcgagtca
     1441 ccctcgggag gagacccatg aagtcgaaac tgacgaccat gctggcgttc gcgctggcgg
     1501 catttgcggg ggccgcgctg gcccagtcga acgtggtcac gatctatgcg gcggacggcc
     1561 tgcacgacgg caatccgagc tggttcggcg atcagttcgc cgcattcacc aaggcgaccg
     1621 gcatcaaggt gcagtacatc gaggcgggct cgagcggcgt ggccgatcgc atcgccaagg
     1681 agaaatcgaa cacgcaggcc gacgttctcg tcacgctgcc gccgttcatg cagcgtgcgg
     1741 ccgccgacgg gctgctgcag ccgtacacgc cggcgggtgc cgacaagctg gccgagcgcg
     1801 acaaggatcc caagggtctc tacgtcgcga tgatgaacaa ctacccgaac ttcatctaca
     1861 acgcgtccgc gttgaaggat gcgccgaaga cctatgaaga cctgctcgac ccgaagtttc
     1921 gcaagaagct gcagtattcg acgccgggac aggccggcga cgggacggcg ctgatgctgc
     1981 aggcgttcca cgcgttcggc agcaaggaag ccggactcgc ctatctcggc aagctgcagg
     2041 cgaacaacct cggaccctcg gcgtcgacgg gcaagctcac cgcgctcgtg aacaagggtg
     2101 agatctacgt cgcgaacggc gacctgcaga tgaacctcgc gcagatgcgc aacaacccga
     2161 acgtgcaggt gttcttcccc gccggtccca acggcaagcg ctcggtgttc gcactgccgt
     2221 acttcatcgg cctcgtcgcc ggcgccccgc acgccgacaa cggcaagaag ctgatcgact
     2281 tcctgatgag ccaggaagcg cagagcacga tcagctcggt cgcgcagggc tttccggtgc
     2341 gcagcgacgt gcatccgtcc gacgagaact tcaagaagct gaacgcgatg ctcgaaggcg
     2401 tcgagatctg gtcgcccgac tggagcaagg tgctgtccga cctgcaggcg gacgtcgcgg
     2461 cctacaacaa ggcgatctcg ggcaattgat gccggccgat ttcgccggcg ccgccgtggc
     2521 ggtgccggct tccacgcgtg ccggcgcgaa gcgcgccggc gcgagcagca gcggtatcgg
     2581 cttcgaggac gtcagcgtcg cctacaacgg cgttgtcgtc ctcgatgcgc tgacgcttgc
     2641 ggtgaatccc ggcgagatcg tcgcgctgat cggcccatcc ggctcgggca agaccacggc
     2701 gctgcgggcg gtcgcggggt tcgtgcggcc gacccgcggc cgcatcacga tcggcacgcg
     2761 cgacgtcacc aacgtcgcgc cgcatgcgcg caacatcggc atggtcgtgc agaactacgc
     2821 gctgtttccc catatgcggg ccgaggagaa cgtcgcgttc gggctgcggg cgcgtggcgc
     2881 gggcgccgac gtcatccgcg agcgggtccc cgaatgcctg cgtctcgtgg gcatggcgag
     2941 ctttgctgcg cgctacccgc gcgagctatc cggcggccag cagcagcgcg tggcgattgc
     3001 gcgcgcgctg gcgattcgcc cgcaggtcct gctgctcgac gagccgctgt cggcgctcga
     3061 cgcgcagata cgccgctcga tgctcgacga gctcgcgaag ctgcaccgtg agctgccgtc
     3121 gctcaccgtc ctctacgtca cgcacgacca gaccgaggcg ctcacgctgg ccgatcacat
     3181 cggcatcatg cgcgacggca agctcgtcgc attcggcgac gcgcagtcgc tgtaccgcta
     3241 tccacccaat cgcttctcgg ccgagttcct cggccgcgcc aacctgcttc ccgtgcaggc
     3301 gcttgccggc gcgggcgccg gcatggcgcg cgtgcgtttc ggcgagggtg cgctgcttgc
     3361 gcgcaaccac catgggctgc ccgacggcgg ccactgcctg ttgtgcgtgc gaccgcacga
     3421 cgtgtcgctc gcgccgccgg aaaaagacgc aaacgcgctc gccgcaacgg tgcgcagcgt
     3481 gcagtggcag ggcgacatgc agaacgtcga gctcgacgtc ggcggccatg tgttgcggct
     3541 gtcgtgcccg ccgatgcacg agcctccgcc acccggcgcg gccttgaccg ttcacttcag
     3601 cgcgacgcag gcgacactcg tcccggagac gatggcccgt ggctgacctt gcgctaggcg
     3661 cgccgtacgc ggcgcaaccg cgcacgagca gtttctggat cgcgccgccg ctgctgatac
     3721 tcgcggcgct tttcttcttc ccgctgtggc tgatcctgcg ccaggcgctc gcgggcgacg
     3781 gcgacgtgct gtcgctcgcc aaattcgtcg gcgtcatcgc ttcgggcgag ttccgccacg
     3841 cgctcgtcaa cacgatcgtc atcgcgctat ccgcgacggc cggctgcctc gtcctcgggt
     3901 tcgtgctgtc ggtgctgatc gcgttcacgc cgtttccggg aagcgaggtc ctcgcacgga
     3961 tgatcgacac gttcatcgcg ctgccgacgt ttctcgtggc actcgcgttc acgttcatct
     4021 acggctcggc cggcatgctc aacgaatcgc tgaaggtgct gctgcatctc gagctgcccc
     4081 cggtcgactt cctgtattcg caatggggcg tgatcctgtc ggaggtgacc gtctatacgc
     4141 cgttcgtgat gcggcc
//
LOCUS       DASSVX010000044        12528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1401391, whole genome shotgun sequence.
ACCESSION   DASSVX010000044 DASSVX010000000
VERSION     DASSVX010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12528
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401391"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VFO53_02110"
     CDS             <1..530
                     /locus_tag="VFO53_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET8875097.1"
                     /translation="DGTVNYASRLPWFSVTMAYQEEGRTRVGVLHAPLAGIVARYVED
                     TVATVNGHDVRVSPCTSLGDAVVSIILTSHFSEREVARAAEAIRRLGNVARGVRIIVS
                     GGLELTLVADARLDAYVSLKADIVSHAAAMPLVRAAGGRVTRFDGRDSTDDDLEKIAS
                     NGAIHDELLACLAGV"
     gene            556..1950
                     /locus_tag="VFO53_02115"
     CDS             556..1950
                     /locus_tag="VFO53_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET8875098.1"
                     /translation="MSSPESATATDARALLDAAVRSASVTRSVDVDPGAIARLPQVLA
                     RLGQRGPCRLVADPNTMAAAGRRTLATLHAANIAIADPIVLEEAPRLKPRAETAREVA
                     SRLRADAALPIAVGAGVINDVTKYAAEVAGLPYVSVVTAASMDGYAASGASMLDAGFK
                     RTLACAPPIGVIADLDVVADAPARMAAWGYGDLSGKLVAGADWTLADALGEDPIDRGS
                     FALVQDHVKGWLAGYDGIRARRTEALRGLVDGLLIAGFAMQAHGNSRPASGSDHQIAH
                     VWEMERLCVDGEPVAHGACVGIGSVAMLALYEWFVAQDVAGRAAEREHAPPVDRDAIE
                     AELQASFPEAALVESARAEMEAKLSRAQLRTARLRALQSGWPALRARIEARIVPAAAL
                     QRWLAACGAASDPRDVGVPLRKLANDYRRARLIRRRYTLLDCLEDLGWLDTAIDALFA
                     QDGFWGRGAPTSPA"
     gene            2001..2819
                     /locus_tag="VFO53_02120"
     CDS             2001..2819
                     /locus_tag="VFO53_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875099.1"
                     /translation="MRDTTVSAWRREVSGPQAWVIGTLCVVAIVGAWWFVTRPGGAIS
                     PLSLPPPEDVADAFVRLLKRPYLGSTLAQHVGASLSVALGGWLAAGIVGLPLGIAMGW
                     WRRMRWIAFPIFQLLRPVPPLAWIPLAILWLGIGESARIFVVFVAAVVPWVMNSMQAV
                     YSIDGLLVRAALTLGASDRQILTRVVCRTALPTLVAGARIALGNAWTTLVAAELLAAT
                     AGLGYIAVNASRTLETGILLVAMGIIGFLGAMFSMAMHWVTRILAPWSRAGASA"
     gene            2879..3646
                     /locus_tag="VFO53_02125"
     CDS             2879..3646
                     /locus_tag="VFO53_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875100.1"
                     /translation="MLYGAVAFAALLAAWIAMTSIPGLVPARLLPSPLEVVERFALLV
                     VEPFSGSTLAGHAVASLQRWLIGVAAALCIGIPVGIVLAWLPPIRAAITPVFELLRYI
                     PPFAWVPIAVLWLGATTTSQALIVFIAAFPACVINTQLGVGQVDRILFHAAKALGAGS
                     LTMLARVALPVAAPSIFTGVRIAFSNGWMALVGAELIVGKQGLGYLIAQGQQNDSAAT
                     ILVGMIAIGIVGALIDVGLQRSQRTLLPWKSTTARPA"
     gene            3616..4485
                     /locus_tag="VFO53_02130"
     CDS             3616..4485
                     /locus_tag="VFO53_02130"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875101.1"
                     /translation="MEIDDRTAGVSAADPGRDAATDVRATRAARPPKMEIKGISKTFA
                     TRNGDVAALDRCDLAIRDGEFVTVVGPSGCGKSTLMMIAAGLEPPSTGEILVDGLAAG
                     PAGPKRSVVFQRFALFPSETVAQNIEFGLRVAGIDRTERETRLAEQLDLMNLRQFRRA
                     YPHELSGGMQQRVAIARALVVRPEILLMDEPFGALDAQTRTVLQEEVSRLRRVMNYTV
                     MFITHSVEEAVYLGDRVVVMSSRPGRIKKDIAVPRDAEWRGLEIEEASNYPEFIRFKR
                     EVWNLVRTEIVRQ"
     gene            4541..5575
                     /locus_tag="VFO53_02135"
     CDS             4541..5575
                     /locus_tag="VFO53_02135"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8875102.1"
                     /translation="MKSSHRIAAFRLALAACIVAVAHPGASIAQNKAPVHVVMISQAN
                     DAGFPIWLARKLDYFAKNGIDAKIQYFPNGGAALASGAAGDWQAGWIGAPPAITGWAK
                     FGLIPVGTMMKEDRNIKLVMRKDALKGSSPRDVLTTKPIGTVPNSTWSQVLFACAKHF
                     GVDPGRMKIVPLEPAVTRQSLRSGDVASGMTDSSADYDLVKDTASFEVVCDGAIGKTS
                     VVDPWVVTRKFASEQPEAAARFVDAVFKADEYINAHREEALQYMMQFYKDAGIDGSLE
                     KARFSLGFRDFQTLDQALADMKSGATEKALTGTAQVFVAGGAYDKVPDLASAIKAGLP
                     ILEAAKKLRK"
     gene            5604..7871
                     /locus_tag="VFO53_02140"
     CDS             5604..7871
                     /locus_tag="VFO53_02140"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET8875103.1"
                     /translation="MGNERRASRDDSVFEGTIGRTWQESTPWWPQIVRPRPGSPNVVV
                     VLLDDTGYGSLGCYGSEIETPNIDALAARGLVYTRFHTTSLCSPTRASLLTGRDHHAV
                     GMSIIANADSGFPSKRGAVSNRAGMLSEILRDAGYNTFAVGKWHLAPADQTSAAGPFD
                     QWPLGRGFEQYYGFLDAVTDQFQPELVHDNHRVDPPRTPEQGYTLNADLVDHAMQYLT
                     DQVSLAPERPFFLYFATGATHSPHEAPSAFREKYRGRYDTGWDVIRERRLAKQKLLGI
                     VPQDTELAPRNPGVAPWESLSADQKRLYARFQEAFAAFLDHTDHEIGRLIRHLERLGR
                     MENTIFVFASDNGASQEGQIHGSTSTTFYENGDAETLDYNLARIDLIGTQHARNNYPV
                     GWAQAGNTPLKRYKQNTHAGGILDPLIVTWPAGIAKPGLRHQFHHAMDIVPTILDALD
                     IEAPAEIRGVPQMPIHGTSMLYSFEGDGPTRKRSQIFEMFGHRGIWHDGWKAVAYHPR
                     YAAYEDDVWELYHVADDVAENRDLAATHPDKLAKLIELWWSEAGRYDVLPLDDRGFAE
                     RRAASRRRPDSPRMRSRYVYYGGMGHVPSGATPFILDRSYSIAADVTLRPGDEGVIIA
                     CGGVCGGYSLYVKDGKVAHDYNYYQHIYRVEADLHPTGKPQRIAYAFTKTGPLAGIGK
                     LYVDNVMVSQTQMAATSRYFMDWEGLDVGRDGLSPVSPAYRGRGEFRFSGEIDRVTVE
                     LRDDVEGVGDFEPSD"
     gene            complement(7879..9447)
                     /gene="betC"
                     /locus_tag="VFO53_02145"
     CDS             complement(7879..9447)
                     /gene="betC"
                     /locus_tag="VFO53_02145"
                     /EC_number="3.1.6.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline-sulfatase"
                     /protein_id="HET8875104.1"
                     /translation="MTTPRPNFLFIMADQLSAQALPSYGHPIVKAPHIDALARDGVLF
                     ENAYCNFPFCAPSRIAMLSGQLPSRIGAFDNAAEMSSSIPTILHYLRAQGYHTSLSGK
                     MHFIGPDQLHGYEERLTTDIYPADFATTPDWSIKGLPVSGISFRGVVEAGICHRNLQI
                     DFDEDVHHEGIRKLYALARTRPQRPFMLTVSYTHPHNPFNTTQAYWNRYDHDAIDPPT
                     VPAIALDDRDPHSRRHYYLTRMDEYDVTAERIRTARHAYYGMVSYFDDKVGELLSVLD
                     DTGLAENTVVVLTADHGEMLGERGMWFKMCLFERAMRVPLILRLPPALRKPRRVARNV
                     SHLDLLPTLLDLASGGKPQPLVEPIDGQSLAPLLEHGGDVPERDVFAEYTAEGAIAPC
                     FMVRSGRHKYIWSEPDGGQLFDLVRDPHEIDDLAGKASAAELEESMLRKVHARWNVAA
                     VERDVLKSQRRRLFLQPVLTFANSHGWDYQPFRDASLEYVRGKTATNAKALSRIPYAE
                     PVEPDHPRASTAND"
     gene            9705..10433
                     /locus_tag="VFO53_02150"
     CDS             9705..10433
                     /locus_tag="VFO53_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8875105.1"
                     /translation="MALAVVSTTMRPVPRRPRFLLTDLPLHVVQRGNDRRPCFLREQD
                     FGVYLSALAEACRQYGVLVHAYVLMTNHVHLLVTPRVAGAVSRTMQSVGARYVRYVNR
                     HSSRSGTLWEGRYHACLVASDEHVLAACRYIDLNPVRARLVQRAADYRWSSYGALAGL
                     HLDALVTPHAVLEQLGSPGGAAYERWCADGDRDADLRALRKATAGDLAFGSDWFKACV
                     AATTSRATHNKPPGPRPTLMKARR"
     gene            complement(10540..11517)
                     /locus_tag="VFO53_02155"
     CDS             complement(10540..11517)
                     /locus_tag="VFO53_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET8875106.1"
                     /translation="MSEAADFRLDEAARAGWLYYIAGKTQDDIARILNVSRPTAQRLV
                     SLCRSEGLVTFRMNHPVGECMRLAASLAERYGLVHCDVVPADGTPDSARAGIAEAGAV
                     YIERVMRSPRAVVMALGTGRSMRASVERVAPMSRSLHRLVSLVGNISPDGSASPFDAL
                     VKLAEISGARHFPMPLPLHAATAAQRDLLLGIPSVRRLFAMANRADVWMMGIGGVDRN
                     AVLLRDGFVTRGELLGAMRAGGVGEITGCVFDADGRILDGGLNARLMSVPLQVDNPRP
                     RICIAHGPDKVVPLRAALTGRLVNGVVTDEITARTLLAPDARPRTRRKR"
     gene            complement(11514..12269)
                     /locus_tag="VFO53_02160"
     CDS             complement(11514..12269)
                     /locus_tag="VFO53_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET8875107.1"
                     /translation="MTDHPPLPPAAAAGASRTFDLVIFDCDGVLVDSEIISCLTVARM
                     LERHGVRCDVTTVLTRYLGRPASTVIDDFSRLARRPLPPGFVDDWRAELFAAFARDLV
                     PMPGAREAVEGLRLSAIDYCLASSSDEERIEVALRKAGLFDFFEGRIFSTTMVERGKP
                     APDLFLLAAATRGAAPGRCVVVEDSVAGVVAARAAGMTAVAFTAGSHYRVMDQTQPLL
                     DAGASCVAASMPELRLLLARMSAAGVAPADAWR"
BASE COUNT         1981 a         4168 c         4319 g         2060 t
ORIGIN      
        1 tcgacggcac ggtcaactac gcgtcgcggc ttccgtggtt ctccgtgacg atggcgtatc
       61 aggaagaggg ccgcacgcgc gtcggcgtgc tgcacgcgcc gctcgccggc atcgtcgcgc
      121 gctatgtcga ggacacggtc gcgacggtga acggccacga cgtgcgcgtg tcgccctgca
      181 cctcgctcgg cgacgcggtc gtgtcgatca tcctcacgtc gcacttcagc gagcgcgaag
      241 tggcgcgtgc cgcggaagcg atccgccggc tcgggaacgt ggcgcgcggc gtgcgcatca
      301 tcgtgtccgg cggcctcgag ctgacgctcg tcgccgatgc gcgactcgac gcgtacgtct
      361 cgctcaaggc cgacatcgtc tcccacgcgg cggcgatgcc gctcgtgcgc gcggccggcg
      421 gacgcgtcac gcgattcgac ggccgcgatt cgacggacga cgatctcgag aagatcgcgt
      481 cgaacggcgc gattcacgac gagctcctcg cgtgtctcgc cggcgtctga tgcgccgcgc
      541 ggccttcccg ctcgcatgag ctcgccggaa tccgcaacgg cgaccgacgc cagggcgctg
      601 ctcgatgcgg cggtacgcag cgcgtcggtc acgcggtcgg tcgacgtcga tcccggtgcg
      661 atcgcgcgct tgccgcaggt cctcgcgcga ctcgggcagc ggggaccgtg ccggctcgtc
      721 gcggatccca acacgatggc ggccgcagga cggcgcacgc tcgcgacgct gcacgcggcg
      781 aacatcgcga ttgcggatcc gatcgttctg gaagaggcgc cgcgcctgaa gccgcgcgcc
      841 gagacggcgc gggaggtcgc ttcgcggctt cgcgccgatg ccgcgctgcc gatcgcggtc
      901 ggcgcgggcg tcatcaacga cgtcacgaag tacgccgccg aagtcgcggg cctgccctac
      961 gtcagcgtcg tcaccgctgc gtcgatggat ggctatgccg catcgggtgc ttcgatgctc
     1021 gacgccggct tcaagcgaac gctcgcctgc gcgccgccga tcggcgtgat cgccgacctc
     1081 gacgtcgtcg ccgatgcgcc ggcgcgcatg gcggcatggg gctacggcga tctctcgggc
     1141 aagctcgtcg ccggtgccga ctggacgctt gccgacgcgc tcggcgagga tccgatcgat
     1201 cgtggctcgt tcgcgctggt gcaggaccac gtgaaggggt ggctcgccgg gtacgacggc
     1261 atccgcgcgc ggcgcaccga ggcgctgcgc gggctcgtcg acggcctgct gatcgccggc
     1321 ttcgcaatgc aggcccacgg caactcgcgg cccgccagcg gcagcgatca ccagatcgcg
     1381 catgtctggg agatggagcg tctttgcgtc gacggcgagc cggtcgcgca cggcgcgtgc
     1441 gtcggtatcg gctcggtcgc gatgctcgcg ctgtatgaat ggttcgtcgc acaggacgtg
     1501 gcgggccgtg ccgcggaacg cgaacatgca ccgcctgtcg accgcgacgc gatcgaagcc
     1561 gagctccagg cgtcgtttcc cgaggcagcg ctcgtggaaa gcgcacgcgc cgagatggaa
     1621 gcgaagctgt cgcgcgcgca attgcggaca gcgcgccttc gtgcgctgca gagcggatgg
     1681 cccgcgttgc gtgcgcggat cgaggcgcgc atcgttcccg ccgcggcgct gcagcggtgg
     1741 ctcgccgcct gcggcgcggc atccgacccg cgcgacgtgg gcgtgccgct gcgcaagctc
     1801 gcgaacgact atcggcgtgc gcgcctgatc cgccggcgct acacgctgct cgattgcctc
     1861 gaggacctcg gctggctcga cacggcgatc gacgcgctct tcgcgcaaga cggcttctgg
     1921 ggtcgcggcg cgccgacgtc gcctgcgtga ccggcacgcc gatggctcgc gcggatttac
     1981 acgcacgatc gcgaggcgct ttgcgcgaca ccaccgtttc ggcctggcgt cgcgaggtca
     2041 gcgggccgca ggcatgggtc atcgggacgc tctgcgtcgt ggcgatcgtc ggcgcatggt
     2101 ggtttgtcac gcggcccggc ggcgcgattt ctccgctgag cctgccgccg ccggaggatg
     2161 tcgccgatgc gttcgttcgc ctgctgaagc ggccctatct cggcagcacg ctcgcgcagc
     2221 acgtcggcgc gagcctgtcg gtggcgctcg gcggatggct cgcggcgggc atcgtcgggc
     2281 ttccgctcgg catcgcgatg ggatggtggc gcaggatgcg ctggatcgcg tttccgatct
     2341 tccagctgct gcgtccggtg ccgccgctcg cctggatacc gctcgcgatc ctgtggctcg
     2401 gcatcggcga gtccgcgcga atcttcgtcg tgttcgtcgc cgcggtcgtg ccgtgggtga
     2461 tgaactcgat gcaggccgtg tattcgatcg acggcctcct cgtgcgcgca gcgttgaccc
     2521 tcggcgccag cgatcggcag atcctcaccc gcgtggtatg ccgcaccgct ttgccgacgc
     2581 tcgtcgccgg cgcacgcatt gcgctcggca acgcgtggac gacgctggtg gcggccgagc
     2641 tgctcgcggc cacggcgggc ctcggctaca tcgcagtcaa cgcgtcgcgc acgctcgaaa
     2701 ccggcatcct gctcgtcgcc atgggcatca tcgggtttct gggagcgatg ttctcgatgg
     2761 cgatgcattg ggtcacgcgc atccttgcac cctggtcgcg cgccggggca tccgcgtgaa
     2821 cggcgccatg ggtaagcgcg tgcatgggcg ccggtggatt gcggacgcga acgggcccat
     2881 gctctacgga gccgtcgcgt tcgcggcgct tctcgcggcg tggatcgcga tgacgtcgat
     2941 accgggactc gttccggcgc gactgctgcc ctcgccgctc gaggtggtcg agcgctttgc
     3001 gctgctggtc gtcgaaccgt tcagcggcag cacgctcgcc gggcatgcgg tcgcatcgtt
     3061 gcagcgctgg ctcatcggcg tcgcggcggc gctgtgcatc ggcattcctg tcggcatcgt
     3121 gctcgcgtgg ttgccgccga ttcgcgccgc catcacgccg gtgttcgagc tgctgcgtta
     3181 catcccgccg ttcgcctggg tgccgatcgc cgtgctgtgg ctcggcgcga cgaccacgtc
     3241 gcaggcgctc atcgtgttca tcgccgcctt cccggcatgc gtgatcaaca cgcagctcgg
     3301 cgtcgggcag gtcgaccgca tcctgttcca tgcggcgaag gcgctcggcg caggctcctt
     3361 gacgatgctg gcgcgcgtcg cgttgccggt tgcggcgccg agcatcttca ccggcgtgcg
     3421 tatcgcgttc agcaacggct ggatggcgct cgtcggcgcc gagctcatcg tcggcaagca
     3481 gggcctcggc tacctgattg cgcagggaca gcagaacgac tcggcggcta cgatcctcgt
     3541 cggcatgatc gcgatcggga tcgtcggcgc gctgatcgac gtggggctgc agcggtcgca
     3601 gcgaacgctc ctgccatgga aatcgacgac cgcacggccg gcgtgagcgc ggccgacccg
     3661 ggacgggacg cggcgacgga cgtgcgggcg acgcgggctg cgcggccgcc gaagatggag
     3721 atcaagggaa tctcgaaaac attcgctacg cgcaacggcg acgtcgcggc gctcgatcgt
     3781 tgcgatctcg cgattcgcga cggcgaattc gtcaccgtcg tcggaccgtc gggctgcggc
     3841 aaatcgacgc tgatgatgat cgctgcgggc ctcgagccgc cgagcaccgg cgagattctc
     3901 gtcgacggcc tcgcagccgg accggcgggt ccgaagcgca gcgtcgtgtt ccagcgcttc
     3961 gcgctgtttc cttcggaaac cgtggcgcag aacatcgagt tcgggttgcg cgtcgccggc
     4021 atcgatcgca ccgaacgcga aacgcggctc gccgagcagc tcgatctcat gaacctgcgg
     4081 caattccgcc gcgcctatcc gcacgagctg tccggcggca tgcagcagcg cgtggcgatt
     4141 gcgcgtgccc tcgtcgtgcg tcccgaaatc ctgctgatgg acgagccgtt cggcgcgctc
     4201 gatgcccaaa cgcgcaccgt gctgcaggag gaggtctcgc ggcttcgccg tgtgatgaac
     4261 tacacggtga tgttcatcac gcacagtgtc gaggaggcgg tgtacctcgg cgaccgcgtc
     4321 gtcgtcatgt ccagccggcc ggggcggatc aagaaggaca ttgcggttcc ccgcgatgcc
     4381 gaatggcgcg ggctcgagat cgaggaagcg agcaactacc ccgaattcat ccgcttcaag
     4441 cgcgaggtct ggaatctcgt gcgcaccgag atcgtgcgtc aataaccatc gttcgtccgt
     4501 cccggaatca cccgttggtt cgctattgga ggagaccgtc atgaaatcgt cgcaccgcat
     4561 cgccgcattc aggctcgcgc tcgccgcgtg catcgtcgcc gtcgcgcatc cgggggcgag
     4621 catcgcgcaa aacaaggcac ccgtgcacgt cgtgatgatc agccaggcga acgacgcagg
     4681 cttcccgatc tggcttgcac ggaagctcga ctacttcgcc aaaaacggca tcgacgcgaa
     4741 gatccagtat ttccccaacg gcggcgcggc gcttgcgtcc ggcgccgccg gcgactggca
     4801 ggcgggatgg atcggcgcgc cgcccgccat caccggctgg gccaagttcg ggctcattcc
     4861 ggtcggaacg atgatgaagg aggaccgcaa catcaagctc gtcatgcgca aggacgcgct
     4921 gaaaggaagc agcccgcgcg acgtgctgac gacgaagccg atcggcaccg tgccgaacag
     4981 cacgtggagc caggtgctgt tcgcgtgcgc gaagcacttc ggcgtcgatc ccggccgcat
     5041 gaagatcgtg ccgctcgagc ccgccgtcac gcgccagtcg ctgcgatcgg gcgacgtggc
     5101 ttcgggcatg accgattcgt ccgccgatta cgacctcgtg aaggacacgg cgagcttcga
     5161 ggtggtgtgc gacggcgcga tcggcaagac gtcggtggtc gatccgtggg tcgtgacgcg
     5221 caagttcgcg agcgagcagc cggaggccgc ggcgcgcttc gtcgacgccg tcttcaaggc
     5281 cgacgaatac atcaacgcgc atcgcgagga ggcacttcaa tacatgatgc agttctacaa
     5341 ggacgccggc atcgacggca gcctcgagaa ggcgcgcttc agcctgggct ttcgcgactt
     5401 ccagacgctc gaccaggcgc tcgccgacat gaagagcggc gccaccgaaa aggcgctgac
     5461 cggcacggcg caggtgttcg tcgccggcgg cgcgtacgac aaggtgccgg accttgcgag
     5521 cgcgatcaag gcggggctgc cgatcctcga ggcggcgaag aagctgcgca aatgaacgaa
     5581 gtcactcgac gggaaggtta gcaatgggca acgagcgccg cgcgtcgcgc gacgacagcg
     5641 tgttcgaggg aacgatcggc cggacatggc aggaatccac gccctggtgg ccgcagatcg
     5701 tgcggccgcg gccgggcagt cccaacgtcg tcgtcgtgct gctcgacgac accggctacg
     5761 gcagcctcgg ctgctacggc agcgagatcg aaacgccgaa catcgatgcg ctcgccgcac
     5821 gcggcctcgt gtacacacgc tttcacacga cgtcgctgtg ctcgcccacg cgcgcgtcgc
     5881 tcctgaccgg acgcgaccat cacgcggtcg gcatgtcgat catcgccaac gccgacagcg
     5941 gctttccgag caagcgcggc gcagtctcga accgggcggg catgctgtcg gagatcctcc
     6001 gcgacgcggg gtacaacacg ttcgcggtcg gcaagtggca tctcgcgcct gccgaccaga
     6061 cgtcggcggc gggtccgttc gaccagtggc cgctcggccg cggcttcgag cagtactacg
     6121 gattcctcga cgcggtgacc gatcagttcc agccggagct cgtgcacgac aatcatcgcg
     6181 tcgatccgcc gcgcacgccg gagcagggct acacgctgaa tgcggatctg gtcgatcatg
     6241 cgatgcagta cctgaccgac caggtgtcgc tcgcgccgga gcgcccgttc ttcctctatt
     6301 tcgcgacggg cgccacgcac tcgccgcacg aggcgccgtc cgcgttccgc gagaaatacc
     6361 gcgggcgcta cgataccggc tgggacgtga tccgggagcg gcgcctcgca aagcagaagc
     6421 tgctcgggat cgtgccgcag gataccgagc ttgcgccgcg caatccgggc gtcgcgccct
     6481 gggagtcgct gtccgcggac cagaagcgct tgtatgcgcg ttttcaggag gcgttcgccg
     6541 cgttcctcga tcacaccgat cacgagatcg ggcgccttat ccgccatctc gagcgcctcg
     6601 gcaggatgga gaacacgatc ttcgtcttcg cgtccgacaa cggcgccagc caggaaggcc
     6661 agatccacgg ctcgacgagc accacgttct acgagaacgg cgatgccgag acgctcgact
     6721 acaacctcgc gcgcatcgac ctcatcggca cgcagcacgc gcgcaacaac tatccggtgg
     6781 gctgggcaca ggccggcaac acgccgctca agcgttacaa gcagaacacg catgcgggag
     6841 gaattctcga tccgctgatc gtcacctggc cggcgggcat cgcgaagccg gggttgcgtc
     6901 accagttcca tcacgcgatg gacatcgtgc cgacgatcct cgacgcgctc gacatcgaag
     6961 cgcccgccga aatccgcggc gtgccgcaga tgccgatcca tggcacgagc atgctgtaca
     7021 gcttcgaagg cgacgggccg acgcgcaagc gctcgcagat cttcgagatg ttcggccatc
     7081 gcggcatctg gcacgacggc tggaaggcgg tcgcctatca cccgcgatat gctgcatacg
     7141 aggacgacgt ctgggagctc tatcacgtcg ccgacgacgt cgcggagaac cgtgatctcg
     7201 ccgcgacgca tccggacaag ctcgcgaagc tgatcgagct gtggtggagc gaggcggggc
     7261 gctacgacgt gctgccgctc gacgaccgtg gattcgccga gcggcgcgcg gcatcgcggc
     7321 gccggcccga ttcgccgcgc atgcgctcgc ggtatgtcta ttacggcggc atgggccacg
     7381 tgccgagcgg tgccacgccg ttcatcctcg accgttcgta ctcgatcgcg gccgacgtga
     7441 cgctgcggcc cggcgacgag ggggtgatca tcgcgtgcgg cggcgtatgc ggcggttaca
     7501 gtctctacgt gaaggacggc aaggtcgcgc acgactacaa ctattaccag cacatctacc
     7561 gcgtcgaggc cgacctgcat cccaccggca agccgcagcg gatcgcctat gccttcacca
     7621 agaccggacc gctcgcgggc atcggcaagc tgtacgtcga caatgtgatg gtgtcgcaaa
     7681 cgcaaatggc ggcgacgtcg cgatacttca tggactggga ggggctcgac gtcgggcgcg
     7741 acggactttc accggtttca ccggcctatc ggggccgtgg cgaattccgc ttcagcggcg
     7801 agatcgaccg cgtcacggtc gagttgcgcg acgacgtcga gggtgtcgga gacttcgagc
     7861 cgtcggactg acggttcgtc aatcgttggc cgtacttgcg cgcggatgat cgggctcgac
     7921 cggctccgcg tagggaatcc ggctcagcgc cttcgcgttg gttgcggtct tgccgcggac
     7981 gtactcgagc gacgcatcgc ggaacggctg gtaatcccat ccatgcgaat tcgcgaacgt
     8041 caggacgggc tgcaggaaga gtcggcgccg ctggcttttg agcacgtccc gctcgacggc
     8101 tgcgacattc catcgcgcat gcaccttgcg cagcatcgac tcctcgagct ccgccgccga
     8161 ggccttgccg gcgagatcgt cgatctcgtg ggggtcgcgc acgaggtcga agagctgacc
     8221 gccatccggc tcgctccaga tgtatttgtg acggccgctt cgcaccatga agcacggggc
     8281 gatcgcgcct tcggcggtgt attcggcaaa cacgtcgcgc tccggaacgt cgccgccgtg
     8341 ctcgagcagc ggcgcgaggc tctgcccgtc gatcggctcg accagcggct gcggcttgcc
     8401 gccggaagcg aggtcgagca gcgtcggaag aaggtcgaga tgcgaaacgt tgcgtgcgac
     8461 gcgtcgcggc ttccgcagag cgggcggcaa ccgcaggatg agcggaacgc gcatcgcgcg
     8521 ctcgaagaga cacatcttga accacatgcc gcgctcgccc agcatctcgc cgtggtcggc
     8581 ggtgagcacg acgaccgtgt tctcggcgag accggtgtcg tcgagcacgc tcaaaagctc
     8641 gccgaccttg tcgtcgaagt agctgaccat gccgtaatac gcgtggcgcg ccgtcctgat
     8701 gcgttccgcg gtgacgtcgt attcgtccat ccgcgtcagg taatagtgcc gcctgctgtg
     8761 cggatcgcga tcgtcgagcg cgatcgcggg caccgtcggc gggtcgatcg cgtcgtgatc
     8821 gtagcggttc cagtatgcct gcgtggtgtt gaacggattg tgcggatgcg tgtacgacac
     8881 ggtcagcatg aacggccgtt gcggccgcgt gcgcgccagc gcgtagagct tgcggatgcc
     8941 ctcgtgatgc acgtcctcgt cgaagtcgat ctgcaggttg cgatggcaaa tgccggcttc
     9001 gaccacgccg cggaaactga tcccggacac cggcaggccc ttgatcgacc agtcgggcgt
     9061 cgtcgcgaag tcggccgggt agatgtcggt cgtcagccgc tcctcgtagc cgtgcaattg
     9121 atcgggcccg atgaagtgca tcttcccgga gaggctcgtg tggtagccct gcgcgcgcag
     9181 gtaatgcagg atcgtcggaa tgctcgacga catttcggcg gcattgtcga aggcgccgat
     9241 cctcgacggc agctggcccg acagcatcgc gatgcgcgac ggcgcgcaga acggaaaatt
     9301 gcagtacgca ttctcgaaca ggacaccgtc gcgggcgagc gcgtcgatgt gcggtgcctt
     9361 gacgatggga tgcccatacg acgggagcgc ctgcgccgaa agctggtccg ccatgatgaa
     9421 caggaagttc ggccgaggtg tggtcatgtg catctcgctt gcggatcgaa gtgccggaat
     9481 atatagatga ttgcggtcca agacaaatgt atcggcgatc acgaagacgt tgatcgtgtt
     9541 tgacttcgag ggcgtcgtcg ccgacagcga gatcctcgcc aacacggcgc tcgctcgagt
     9601 tcggggtcag agctgtaatt gtcgcgtttg acgtcgagtt cggggtcgga gctggcgatc
     9661 tgcggcgcac ttgttcggga tccccggttc ggtcaatcgg ctgattggcg ctcgccgttg
     9721 tttcaacgac catgcggcct gtgccacgcc gtccccgctt cctccttacc gatttgccgc
     9781 tgcatgtcgt gcagcgcggc aatgaccggc gtccctgctt cctgcgagag caggacttcg
     9841 gcgtctacct gagtgcgctc gctgaagcat gcaggcagta cggcgtgctc gtgcacgcgt
     9901 acgtcctcat gaccaatcac gtccatctgc tggtaacgcc ccgcgtcgcg ggcgcggttt
     9961 cgcggacgat gcagtcggtg ggcgcgcgct acgtccgtta cgtgaaccgg cattcgtccc
    10021 gaagcgggac gctctgggag gggcgctacc atgcgtgtct cgtagcttcc gacgagcatg
    10081 tgctcgccgc atgtcggtac atcgatctca atcccgtgcg cgcgaggttg gttcagcgcg
    10141 cggcggatta tcggtggagc agctacggcg cgctcgcggg actgcacctc gacgccctgg
    10201 tgacgccgca tgcggtgctt gaacaactcg gcagtccagg cggtgcggcc tatgaacgtt
    10261 ggtgtgccga tggcgatcgc gacgcggacc tccgagcgtt gcgcaaggca accgctggcg
    10321 acctcgcgtt cggctcggac tggttcaagg cgtgcgtggc cgcaacgacc tcgcgcgcca
    10381 cccacaacaa acccccaggt ccgcggccaa cgctgatgaa ggctcggcgg tgaaacccga
    10441 aatcggggtc agagctgtaa ttattctcgc gcgcggatga ttacagctct gaccccgatc
    10501 tcgagcggcg tgcgaataat tacagctctg accccgatct tatctcttgc gccgggtgcg
    10561 cggtcgcgcg tccggcgcga gcagcgtgcg tgcggtgatc tcgtcggtca cgaccccgtt
    10621 gacgagccgg ccggtcagcg cggcgcgcag cggaacgacc ttgtcgggtc cgtgcgcgat
    10681 gcatattcgc ggccgcgggt tgtcgacctg cagcggcacg ctcatgaggc gcgcattgag
    10741 cccgccgtcg aggatgcgtc cgtcggcgtc gaatacgcag cccgtgatct ctccgacgcc
    10801 cccggcgcgc atcgcgccca gcaactcgcc gcgggtgacg aagccgtcgc ggagcagcac
    10861 tgcgttgcgg tcgacgccgc cgatgcccat catccagacg tccgcgcggt tcgccatcgc
    10921 gaacaatcgg cgcacggacg ggatgccgag caacaggtcg cgctgcgcgg cggtcgccgc
    10981 gtgcagcggc agcggcatcg ggaagtgccg tgcgccgctg atttcggcga gcttgaccag
    11041 tgcgtcgaac ggactcgccg acccgtcggg cgagatgttg ccgaccagcg agacgaggcg
    11101 gtgcagcgag cgcgacatcg gcgcaacgcg ctcgacgctc gcgcgcatcg accggccggt
    11161 gccgagcgcc atcacgacgg cgcgcggcga gcgcatcacg cgttcgatgt agacggcacc
    11221 ggcttcggcg atgccggcgc gcgccgaatc cggcgtgccg tccgccggca ccacgtcgca
    11281 gtgcacgagg ccgtagcgct cggcgaggct cgcggcgagg cgcatgcact cgccgaccgg
    11341 atgattcatc cggaacgtga cgagtccctc ggatcggcac aacgagacca gccgttgcgc
    11401 cgtcggccgc gacacgttga ggatccgcgc gatgtcgtcc tgcgtcttgc cggcgatgta
    11461 gtagagccag cctgcgcgcg ccgcctcgtc gaggcggaaa tcggcggcct cgctcatcgc
    11521 cacgcgtccg ccggtgcaac gccggcggcg ctcatccgcg caagcagcag ccgcaattcg
    11581 ggcatcgacg ccgcgacgca cgacgcgccc gcgtcgagca gcggctgcgt ctgatccatc
    11641 acccggtaat gactccccgc cgtaaacgcg acggccgtca tgccggcggc gcgcgcggcg
    11701 acgacgcccg cgacgctgtc ctcgacgacg acgcagcggc ctggagccgc gccgcgcgtg
    11761 gcggcggcga gcaggaagag atccggcgcc ggcttgccgc gctcgaccat cgtcgtgctg
    11821 aagatgcggc cctcgaagaa gtcgaacagc cccgccttgc gcagggcgac ctcgatgcgc
    11881 tcctcgtcgc tcgacgacgc gaggcagtag tcgattgcgg aaagtcgcaa tccctcgacg
    11941 gcttcgcgcg cacccggcat cggcacgaga tcgcgcgcaa acgccgcgaa gagctcggcg
    12001 cgccagtcgt cgacgaagcc cggcggcagc ggccggcgcg cgagacgcga gaaatcgtcg
    12061 atgaccgtgc tcgccggacg tcccagatag cgcgtcagga ccgtcgtgac atcgcacctg
    12121 acgccgtgcc gctcgagcat gcgagcgacg gtcaggcagc tgatgatctc gctgtccacg
    12181 agcacgccgt cgcaatcgaa gatcacgaga tcgaaggtgc gcgaagcgcc ggccgcggcc
    12241 gccggcggta gcggaggatg atcggtcatc ggtgtgagca atcgctcatc gcgcgagcga
    12301 ttgcttgatc atagctgaaa tccgtgtacc atgcgccgtg ctgcccggcg tccggctcgc
    12361 gattcatcca ttgcgaatgg cgacgcccca tggaggaaag gtcgatgagg cttctgttca
    12421 ggctgatgct cgtgctcgca tcgacgctgc tcgcgacgtg catcgcggcc ggcgcggcgc
    12481 gcgcgcaagg tgtaacgctg gttgtgttct acgcgcagcc gtcgttcg
//
LOCUS       DASSVX010000045         5193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1018072, whole genome shotgun sequence.
ACCESSION   DASSVX010000045 DASSVX010000000
VERSION     DASSVX010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018072"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="VFO53_02165"
     CDS             <1..1332
                     /locus_tag="VFO53_02165"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8875108.1"
                     /translation="QVLLFAASLLLGAAALAGIVGPLTLLFLTFLVGAGFTFYLPAQQ
                     ASINEMVSRSDLARAVALGSVAFNVARAVGPAAAGAIAAALSSGATLVLGAFFFVPMF
                     VAMRHTTPREVVLPGVPERLMSGMMSGLRYVRHSAPMRAFIVRNLAFSVCASAFWALL
                     PVIARDQLGLGAGGFGLLSASFGIGAIVGAISIPALLMRQPMNKVVTSAGQLWALAAL
                     IVALTGITALALLGAFCAGMAWVCVFATLSAGTQSSAPAWVRARAVSMNLVATQGCLA
                     IGSAVWGALANGVGTHWALGVSAVSVIVLQWLYRRVRVEMGQEADVVPGVRLPELPIQ
                     TPPLPDDGPVLIQLEYRIDAADRDAFLKAIQKVGPTRRRNGATSWRVFRDLGEEGRFV
                     ERYVIASWAEYVRLRARMTMADSRLQQAVAELQRSGTPIRVSRFIDVTTNV"
     gene            complement(1410..2603)
                     /locus_tag="VFO53_02170"
     CDS             complement(1410..2603)
                     /locus_tag="VFO53_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET8875109.1"
                     /translation="MPQSATKRAPIRIVDVREVTKPIGSPIRNAYIDFTTMTTSLVAV
                     VTDAMRDGKRVVGYGFNSNGRYGQGGLIRERFAPRLLAADPLALCDESGTNLDPDRAW
                     AAMMTNEKPGGHGERSVAVGTLDMAIWDAVAKIAGKPLFRLLAERHGLHADPRVFVYA
                     AGGYYYPGKARDALRAEMKSYVERGYTVVKMKIGGAPIDEDRERIEAVLDAIGSAASL
                     AVDANGRFDLDTAIAYAKMLSGYKLFWYEEAGDPLDFRLQAALAQFYPAPMATGENLF
                     SHQDARNLVRYAGLRPDRDVLQFDCALSYGLCEYQRTLAMLTAHGWSARRCIPHGGHQ
                     MSLNIAAGLGLGGNESYPDLFQPYGGFPDGVHVRDGHVTLPELPGIGFEGKRELYAQM
                     RALAS"
     gene            2640..3422
                     /gene="ygiD"
                     /locus_tag="VFO53_02175"
     CDS             2640..3422
                     /gene="ygiD"
                     /locus_tag="VFO53_02175"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HET8875110.1"
                     /translation="MDARAMPAIFFGHGNPMHALARNAYTDGWSALGRSIPRPKAILS
                     ISAHWYIPGTSVTIADAPATLHDFGGFPRELFAVQYPAPGSRALAGRVRELLAPVRVD
                     DDGRWGLDHGTWSVLVHVYPDADVPVVQLSIDETREASWHYEIAKQLAPLRDEGVLIM
                     GSGNLVHNLHTYSWGNHALGAYDWAQRFEDRARSLLAARDFAPLVDYESLGRDAQLSA
                     PTPDHYLPLVYVLAQYRDGDTVTFPVEGFDGGSISMLSVAIG"
     gene            3443..3775
                     /locus_tag="VFO53_02180"
     CDS             3443..3775
                     /locus_tag="VFO53_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HET8875111.1"
                     /translation="MIGLPEIAAILACALVGWLVWDTMKARETANEAMRAACERSGYL
                     FLDDTVSLVSVRPVRDDEGHVRLSRVYTFQYSDTGHNRRNGSITLRASAVTALALASE
                     PPPTDVLS"
     gene            complement(3813..4475)
                     /locus_tag="VFO53_02185"
     CDS             complement(3813..4475)
                     /locus_tag="VFO53_02185"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HET8875112.1"
                     /translation="MHGNGFRLALFALALGFVASAGAQSMQTIRGDVAAVEGKTLRLK
                     SASGEETAIALADNPRVSLRVPTSIDAIKPGTFVGTAATPGPNGTLVASEVHIFPEAL
                     RGTGEGHRPMPTLPGSTMTNATVAGVTPSASKPRGTMTNATVENVGVAGKGRTLKLTY
                     KGGEQTVFVPQSVPVVTVEEGSRASLSPGEHVIVYAKRDASGALAAQRISVGKNGSVP
                     PI"
     gene            complement(4501..>5193)
                     /locus_tag="VFO53_02190"
     CDS             complement(4501..>5193)
                     /locus_tag="VFO53_02190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8875113.1"
                     /translation="PAHWRYAFVTVAAALLVVTLLVAPRLVEPVRLGGSVSSTPLVRL
                     VGRRAQLLAQIAAFGAFGAFSTAFNYFPFYLSRPPWSLSTPTITAFYLVYIAGLFMGP
                     FAGRLGNRFGNGAVMIGGAIVLAGALSLSLVVAVPALVASLIGLCVGFFAIHAAAFGA
                     LNRSLSGERGKANALYTLFYYLGGAAGIGVGGELYARFGWPGIVGVSVTMALLPLAAG
                     GVELRTSARRPR"
BASE COUNT          780 a         1789 c         1780 g          844 t
ORIGIN      
        1 caggtgctgc tgttcgccgc gagcctgctg ctcggcgccg cggcgctcgc gggcatcgtc
       61 gggccgttga cgcttttgtt tctgacgttc ctcgtcggcg cgggcttcac gttctacctg
      121 cccgcgcaac aggcgagcat caacgaaatg gtgtcgcgca gcgacctcgc gcgcgccgtc
      181 gcgctcggtt ccgtcgcgtt caacgtcgcg cgtgcggtgg ggccggccgc ggccggtgcg
      241 atcgccgcgg cgctcagttc gggcgccacg ctcgtcctcg gcgcgttctt cttcgtgccg
      301 atgttcgtgg cgatgcgcca cacgacgccg agggaggtcg tgctgcccgg cgtgcccgag
      361 cgcctgatgt ccggcatgat gagcgggctt cgctacgtgc gccattccgc gccgatgcgc
      421 gcattcatcg tcaggaacct cgcgttcagc gtctgcgcga gcgcgttctg ggcgctgttg
      481 ccggtcattg cgcgcgatca gctcggcctc ggcgccggcg gtttcggcct gctgtcggcg
      541 agcttcggca tcggcgccat cgtcggcgcg atctcgattc ccgcgcttct tatgcgccaa
      601 ccgatgaaca aggtcgtcac gtcggccggc cagctctggg cgctcgccgc actgatcgtc
      661 gcgctgaccg gcatcaccgc gctcgcgctg ctcggggcgt tctgcgcggg catggcgtgg
      721 gtgtgcgtgt tcgcgacgct ttccgcgggg acgcaaagca gcgcaccggc gtgggtgcgc
      781 gcgcgcgccg tgtcgatgaa cctggtcgcg acgcagggct gcctcgcgat cggcagcgcc
      841 gtgtggggtg cgcttgccaa tggcgtcggc acccactggg cgctcggcgt gtcggccgtt
      901 tcggtgatcg tgctgcaatg gctctaccgg cgcgtgcgcg tcgagatggg tcaggaagcc
      961 gacgtcgtgc ccggcgtgcg gctgcccgag ctgccgatcc agacgccgcc gctgcccgat
     1021 gacggtccgg tgctgatcca gctcgagtac cggatcgatg cggccgatcg cgacgccttc
     1081 ctgaaggcga tccagaaggt ggggccgacg cggcggcgca atggtgccac gagctggcgc
     1141 gtgtttcgcg acctcggcga ggaagggcgc ttcgtcgagc gctacgtcat cgcgtcgtgg
     1201 gccgaatacg tgcggttgcg cgcacgcatg acgatggccg acagccggct gcagcaggcg
     1261 gtggccgagc tgcagcgttc cggcacgccg atccgcgtgt cgcgcttcat cgacgtgacg
     1321 accaacgtct gatccaccgg cgcacgccaa cctcgccgcg tcgcgcgcgc cgacagccgc
     1381 gccacacgcg tagcgcgtga cgccgcgcct caggatgcga gtgcgcgcat ttgcgcgtac
     1441 agctcgcgct tgccttcgaa gccgatgccc ggcagctcgg gcagcgtgac gtggccgtcg
     1501 cgcacgtgca caccatccgg aaagccgccg tacggctgaa agaggtcggg gtagctttcg
     1561 ttgccgccga gaccgaggcc ggccgcgatg ttgagcgaca tctggtggcc gccgtgcgga
     1621 atgcagcggc gcgccgacca tccgtgcgcc gtcagcatgg cgagcgtgcg ctggtactcg
     1681 cacaatccgt acgacagcgc gcaatcgaac tgcaggacgt cgcggtccgg gcgcaggccg
     1741 gcgtagcgaa cgagattgcg cgcatcctga tggctgaaga ggttctcgcc cgtcgccatg
     1801 ggcgcggggt agaactgcgc gagcgccgcc tgcaggcgga agtcgagcgg atcgcccgct
     1861 tcctcgtacc agaacagctt atagccggac agcatcttcg cgtacgcaat ggcggtgtcg
     1921 aggtcgaagc gaccgttcgc gtcgacggcg aggcttgcag cgctgccaat ggcatcgagc
     1981 acggcctcga tgcgctcccg gtcctcgtcg atcggcgccc cgccgatctt catcttcacg
     2041 accgtgtagc cgcgctcgac gtagcttttc atttcggcgc gcagcgcatc ccgggccttc
     2101 cccgggtagt agtagccgcc tgccgcgtac acgaacacgc gcggatcggc gtgcaggccg
     2161 tggcgctcgg cgagaagccg gaagagcggc ttgccggcga ttttcgcgac cgcatcccag
     2221 atcgccatgt cgagcgtgcc gacggccacc gagcgctcgc cgtggccgcc cggcttttca
     2281 ttcgtcatca tcgcggccca cgcgcgatcg gggtcgagat tggtcccgct ttcatcgcat
     2341 agcgccaacg ggtcggcagc aaggaggcgc ggcgcgaagc gctcgcggat caagccgccc
     2401 tgcccgtagc ggccgttgga gttgaagccg tagccgacga cgcgcttgcc gtcgcgcatc
     2461 gcatcggtga cgaccgcgac gagactggtc gtcatcgtcg tgaagtcgat gtacgcgttg
     2521 cggatcggcg agccgatcgg cttcgtcacc tcgcgcacat cgacgatgcg aatgggcgcg
     2581 cgcttcgttg cggattgcgg cattgggatc tctcggcggc gaagcgatat tatcggtgca
     2641 tggacgctcg agcgatgccg gcgattttct tcgggcacgg caacccgatg catgcgctag
     2701 cgcgcaatgc gtacaccgac ggctggtcgg cactggggcg ttcgatcccg cggccgaagg
     2761 ccatcctgtc gatttccgcg cactggtaca tcccgggaac gagcgtgacg attgccgatg
     2821 ctcccgccac gctccatgac ttcggcggat tcccgcgcga gctcttcgcc gtccagtatc
     2881 cggcgcccgg ttcgcgggcg ctggccgggc gcgtgcgcga gctcctggcg ccggtgcgcg
     2941 tggacgacga cgggcgctgg ggactcgacc acggaacctg gtcggtgctc gtgcatgtct
     3001 accccgatgc cgacgttccg gtcgtgcagt tgagcatcga cgaaacgcgc gaagcgtcgt
     3061 ggcactacga gatcgcgaag cagctcgcgc cgcttcgcga cgaaggcgtg ctgatcatgg
     3121 gcagcggcaa cctcgtgcac aacctgcaca cctattcctg gggcaatcac gcgctcggcg
     3181 cctacgactg ggcgcagcgc ttcgaggaca gggcgcgctc gctcctcgcc gcgcgcgatt
     3241 tcgcgccgct cgtcgactac gaatcgctcg gacgcgatgc gcaattgtcc gcacccacgc
     3301 ccgatcacta cctgccgctc gtctacgtgc tcgcgcaata ccgcgacggc gacacggtca
     3361 cgttcccggt cgaaggcttc gacggcggct cgatctcgat gctgtcggtc gcgatcggat
     3421 agcgcgtgcg gccgccgttg cgatgatcgg gctgccggaa atcgcggcga tcctcgcctg
     3481 cgcgctcgtt ggctggctcg tgtgggacac gatgaaggcg cgcgagacgg cgaacgaggc
     3541 gatgcgcgcg gcgtgcgagc gcagcggcta tctgtttctc gacgacacgg tgtcgctcgt
     3601 gtcggtacgt ccggtgcgtg acgacgaagg ccacgtccgg ctttcgcgcg tctacacgtt
     3661 ccagtacagc gataccggcc ataaccggcg caacggatcg atcacgttac gcgcaagcgc
     3721 cgtcacggca ctcgcgctcg cctccgagcc gccgccaacc gatgtgctgt cctgaggccg
     3781 gcgacgaatc tgcgcgcacc gtgcacagca cgtcagatcg gcggcaccga tccgttcttg
     3841 ccgacgctga tccgctgggc ggcaagcgcc ccgctcgcat cgcgcttcgc gtagacgatc
     3901 acgtgctcgc cgggcgacag cgacgcccgg ctcccttcct cgaccgtgac gacgggcacg
     3961 ctttgcggca cgaacaccgt ctgctcgccg cccttgtacg tgagcttgag cgtgcggccc
     4021 ttgccggcaa cgcccacgtt ctcgaccgtc gcgttcgtca tcgtgccgcg tggcttcgac
     4081 gccgacggcg tgacgccggc caccgtcgcg ttggtcatcg tgctgcccgg cagtgtcggc
     4141 atcggccggt gaccttcgcc ggtgccgcgc aacgcctccg gaaagatgtg cacctcgctt
     4201 gcgacgagcg tgccgttcgg gcccggcgtt gccgcggtcc cgacgaacgt gcccggcttg
     4261 atcgcgtcga tgctggtcgg cacgcgcagg cttacgcgcg ggttgtcggc gagcgcgatg
     4321 gccgtttcct cgccgcttgc cgacttgaga cgaagcgtct ttccctccac cgctgcgaca
     4381 tcgccgcgga tcgtctgcat cgactgcgca cccgccgagg cgacgaagcc gagcgccagc
     4441 gcaaatagcg ccaagcgaaa cccattgcca tgcatcgtgc tctccttcaa catgcgaaag
     4501 tcagcgtggc cgccgggccg aggttcgtaa ctcgaccccg cccgcggcaa gcggcaacag
     4561 cgccatcgtc accgatacgc cgacgatgcc cggccagccg aagcgcgcat aaagctcgcc
     4621 cccgacaccg atgcccgccg cgccgccgag atagtagaac aacgtataga gcgcgttcgc
     4681 cttgccgcgt tcacccgaaa ggctgcggtt gagcgcaccg aacgccgcgg cgtggatcgc
     4741 gaagaagccg acgcacaggc cgatcaggct cgcaaccagc gccggcaccg caacgacgag
     4801 cgacagcgac aacgcgcccg cgagcacgat tgcgccgccg atcatcaccg cgccgttgcc
     4861 gaaccggttg ccgagcctgc cggcgaacgg tcccatgaaa aggcccgcga tgtacacgag
     4921 gtagaacgcg gtgatcgtcg gcgtggaaag cgaccacggc ggccgggaca gatagaacgg
     4981 gaaatagttg aacgcggtgg agaacgcgcc gaacgcgccg aaggcggcga tctgtgcgag
     5041 cagttgcgcg cgccggccga cgaggcgaac gagcggcgtc gacgatacgg acccgccgag
     5101 gcgcacaggc tcgacgagcc gcggcgcgac cagcagcgtg acgacgagaa gcgccgcggc
     5161 gaccgtcacg aacgcgtagc gccagtgcgc cgg
//
LOCUS       DASSVX010000046         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1384867, whole genome shotgun sequence.
ACCESSION   DASSVX010000046 DASSVX010000000
VERSION     DASSVX010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384867"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VFO53_02195"
     CDS             <1..800
                     /locus_tag="VFO53_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET8875114.1"
                     /translation="SQLLTSTPHDTSGDEQRVLERIKPVGSIVIADASAPKGNLTGEQ
                     VYGQVCKTCHDAGLAGAPKTGDKAQWAPRIAEGEKTLVQHAIAGFQGKVGVMPPKGGN
                     PDLTDDEVHRAVVYIANQAGAGWKEPAPTAVAGAAPAATATAPAQAVASTSTTPATAA
                     AAPVVAAAAAPATATPAATPAAGGAKADGKAVYEKTCAVCHATGLAGAPKFGDKAAWA
                     PRIATGINTLHNSALHGKNAMPPKGGNLALSDADVTAAVDYMAAAAK"
     gene            complement(862..>2095)
                     /locus_tag="VFO53_02200"
     CDS             complement(862..>2095)
                     /locus_tag="VFO53_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET8875115.1"
                     /translation="DNLAALPRDFANLRVIKLEQNYRSTVRILRCANALIGNNPKLFD
                     KRLWTELAHGEPLRVMPAADEEAEAELVVRRLLSHKFEHRQKFSDYAILYRGNHQARV
                     FEAMLRTHSTPYEISGGQSMFERTEIKDVVAYLRLIANDDDDPAFVRALAAPKRGVGA
                     TTLRHLGDVAHATRTSLFGAVFEPSLATSVPARQRAVLEEFCALINGIRHRAEREPAG
                     RLLDEMLKRTGYADWLVATLDRRDAQARTQSVDDFASWLARKGEADGKNLLELTQMVA
                     LITMLEGRDGEAADVVRLSTLHAAKGLEFQHVVLVGLEEGILPHREAIDAGNVDEERR
                     LMYVGLTRAQQSLHLSYCRTRRRGGERIDCVPSRFIAELAQDDVRYADQTLSASEAAS
                     AKQTGNTRLQALKALVAR"
BASE COUNT          375 a          712 c          686 g          322 t
ORIGIN      
        1 tctcacaact gttgaccagc acgccgcatg acacgtcggg cgacgagcag cgcgtgctcg
       61 agcgcatcaa gcccgtgggc tcgatcgtca tcgcggacgc atcggcaccc aagggaaatc
      121 tgaccggcga gcaagtctac ggtcaggttt gcaaaacgtg ccatgatgcg ggcctcgccg
      181 gcgcgccgaa aaccggcgac aaggcgcaat gggcgccgcg gattgcggag ggcgagaaga
      241 ccctcgtgca acatgcgatc gcgggcttcc aaggcaaggt cggcgtgatg ccgcccaagg
      301 gtggcaaccc cgacttgacg gacgacgaag tgcaccgcgc tgtcgtctat atcgcgaatc
      361 aggcgggcgc aggctggaag gagccggcgc ccaccgcggt tgcaggcgca gcgccggccg
      421 cgacggcaac cgcacccgcg caggcggtcg ccagcacgtc gaccacgccc gctaccgctg
      481 cagccgcgcc cgtggtggct gctgcggcag cgcctgcgac cgcgacgccc gcagccacgc
      541 ctgctgcggg aggcgcaaag gcggatggca aagccgtcta cgagaaaacg tgcgccgtgt
      601 gccatgcgac cgggttggcg ggggcaccga aatttggcga caaggccgcg tgggcgccgc
      661 gcatcgcgac gggcatcaac acgctgcaca acagcgcgct tcacggcaag aacgcaatgc
      721 cgcccaaagg gggcaacctc gcgttgagcg atgcggacgt caccgccgcc gtcgactaca
      781 tggcggccgc ggcgaagtag cgctcgacct gcggccggcg attacggcac gccgcgcgcc
      841 gcgccgtgcc gtcgcaacgt ttcagcgggc gacgagcgcc ttcagcgcct gcagccgcgt
      901 atttcccgtc tgcttggcac tcgccgcctc actcgccgac aaggtttgat ccgcataacg
      961 cacgtcgtcc tgcgcgagct cggcgatgaa gcgcgatggc acgcagtcga tcctttcgcc
     1021 gccacggcgt cgtgtccggc aatacgaaag atgcaagctc tgctgcgcac gcgtgagacc
     1081 cacgtacatc agccgccgct cttcgtcgac gtttccggca tcgatcgcct cgcggtgggg
     1141 aagaatcccc tcctcgagcc cgacaaggac cacatgctga aactcgagcc ctttcgccgc
     1201 gtgcagcgtc gatagccgca cgacgtcggc ggcctcgccg tcgcgaccct cgagcatcgt
     1261 gatcagcgca accatctgtg tcagctcgag cagattcttg ccatccgcct cgcccttccg
     1321 cgcgagccag cttgcgaaat cgtcgacgct ttgcgtgcgc gcctgcgcat cgcggcggtc
     1381 gagcgtcgcg acgagccaat cggcgtagcc cgttcgcttc agcatctcgt cgagcagccg
     1441 tccggccggc tcgcgctcgg cgcggtgccg gattccgttg atcaacgcgc agaactcttc
     1501 gagtactgca cgctggcgcg ccggcaccga cgtggcaagc gacggctcga atacggcgcc
     1561 gaagagactc gtccgtgtcg cgtgcgcgac gtcgccgagg tgacgaagcg tcgtcgcgcc
     1621 aacgccgcgt ttgggggccg caagcgcgcg aacgaacgca ggatcatcgt cgtcgttcgc
     1681 gatcagccga agataagcca cgacgtcctt gatctcagtc cgctcgaaca tcgactggcc
     1741 gccggagatc tcgtacgggg tcgaatgggt ccgcagcatc gcctcgaaca cgcgggcctg
     1801 gtgattgccg cgatacagga tcgcgtagtc ggaaaatttc tgccggtgct cgaacttgtg
     1861 cgacaagagg cgccgcacga caagctccgc ctcggcttcc tcatcggcgg cgggcatcac
     1921 gcgcagcggt tcgccatgtg caagctcggt ccacagccgc ttgtcgaaga gcttcggatt
     1981 gttgccgatc aacgcgtttg cacagcggag gatgcgcacc gtcgaccggt agttctgctc
     2041 gagcttgatg acgcgcagat ttgcgaaatc gcgcggcaaa gcggcgagat tgtcg
//
LOCUS       DASSVX010000047         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_468177, whole genome shotgun sequence.
ACCESSION   DASSVX010000047 DASSVX010000000
VERSION     DASSVX010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468177"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFO53_02205"
     CDS             complement(<1..190)
                     /locus_tag="VFO53_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875116.1"
                     /translation="MTGISIAFAALAVIAMSRPLPDAIARAKSDVARSRALVENAQAL
                     VADNESLGRGATPLRGGDI"
     gene            complement(255..1454)
                     /gene="gspL"
                     /locus_tag="VFO53_02210"
     CDS             complement(255..1454)
                     /gene="gspL"
                     /locus_tag="VFO53_02210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HET8875117.1"
                     /translation="MLRVLLAAPPDRDHPSRWVQYAADGRVTGAGNDVPSRWPADASL
                     EVVLAASLARIAVVDLPPMPPNRRHAAARFALEDQLAAGADEAAIAVAPSGASTVVAI
                     ASRALIDGIVANERRVTKIVPEAALAPRNPGWTWCVSGTGDSFVRREDGSAFAIGRAT
                     DDMPPPELRAALAQALRAETAPAVVHAAMNVPPEQLAQWSQASGVRFVAAAPWRWEDA
                     AAATFAAAPDFLATDGSQALTSERPGARLFRPAIVLASLALALHLGASLVQWAWLGIT
                     DWRLSRQLTELARTAGVPTGNASLAAAAIARRNAELRHAAAKPAITDALPLLARAAPA
                     LGALPQGTLKSATYADNTWTFELGRLDAETSSRVIRALGNAGIDALAAPTSSGTRMRA
                     TLDATAR"
     gene            complement(1485..>2307)
                     /gene="gspK"
                     /locus_tag="VFO53_02215"
     CDS             complement(1485..>2307)
                     /gene="gspK"
                     /locus_tag="VFO53_02215"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HET8875118.1"
                     /translation="QAQWTAHVSQRRDLVQAQSIALAGVQWTRQILAMTRASSFNHLG
                     EPWALPLPATPVENGSVEGRIIDAQGLLNVNNLALAGTPANERQRFARAFDALHVAPA
                     TLAAIVDWVDSDAIAEPDGAEDAWYRNAPQPGLAANMPVMRVEELAAVRGVTTSVLAS
                     LTRYVTALPENAPLNVNTAPAELLAASIDGVDAATLAALVASRAQQPFRTITDFHARL
                     PQTATLIGGEPLYTVKSRYFLVWVRARQGDTVAQARALIDCEGAAWPRVVWQTIE"
BASE COUNT          382 a          776 c          793 g          356 t
ORIGIN      
        1 gaatgtctcc cccgcgaagc ggcgtggcgc ctcgcccaag actctcattg tcggcgacga
       61 gcgcctgtgc gttctcgacg agtgctcgag atcgtgcgac atcggacttt gcacgcgcaa
      121 tcgcgtcggg aagcggccgc gacatggcga tgaccgcgag cgcagcaaac gcaatggaga
      181 tgccggtcaa ccagcgccgt tgagtgcgac cattgccaag ccacgaggcg atcctggttt
      241 gcacgtcgat ccgatcagcg cgcggtcgca tcgagtgttg cacgcattcg cgtaccggac
      301 gacgtgggcg ccgccagtgc gtcgatacct gcattgccga gggcacggat cacgcgcgac
      361 gacgtttccg cgtcgagcct gccaagttcg aatgtccatg tgttgtcggc gtacgtagcc
      421 gacttgagcg ttccctgcgg aagcgcgccg agcgcgggtg ctgcgcgcgc gagcaacggc
      481 agcgcatcgg tgatggccgg ctttgccgcg gcgtgccgca actccgcatt gcgccgcgcg
      541 atcgcggcgg ccgccagcga cgcgttgccg gtcggcacgc cggccgtgcg cgccagctcg
      601 gtcagttgcc gtgacaagcg ccagtcggtg atgccgagcc aagcccattg cacgagcgac
      661 gcgccaaggt gcagcgcgag cgcaagcgac gccagaacga tcgccggccg gaacaggcgc
      721 gcaccaggtc gctcgctcgt tagggcttgc gaaccgtccg tggccaagaa atccggcgca
      781 gcggcaaatg tcgccgccgc ggcatcttcc cagcgccacg gcgcagccgc gacgaagcgc
      841 acgccagacg cttgcgacca ttgcgcgagt tgctcgggcg gcacattcat cgccgcatga
      901 acgacggctg gcgctgtctc cgcacgcagc gcctgcgcca gggccgctcg cagttccggt
      961 ggcggcatgt catccgtcgc tcgaccaatg gcaaaggcgc tgccgtcttc gcgcctgacg
     1021 aaactatcgc ctgtgccgga gacgcaccac gtccagccag gattccgcgg tgcgagcgcc
     1081 gcttcgggga cgatcttggt cacacggcgt tcatttgcga cgatgccgtc gatcagcgca
     1141 cgtgatgcaa tcgcaacgac cgttgaagcc cccgacggtg cgaccgcaat ggccgcctcg
     1201 tcggctccag cggctaactg atcctccaac gcgaagcggg ccgccgcgtg ccggcggttt
     1261 ggtggcatgg gcggcaagtc gacgacggcg attcgcgcca gggaagcggc gagcacgact
     1321 tcgagcgaag catccgctgg ccagcgcgac gggacgtcgt tacccgcacc ggtgacgcga
     1381 ccgtcggctg cgtattggac ccagcgactc ggatgatcgc gatcgggggg tgctgcgagc
     1441 aggacgcgaa gcatcggcgc agtgtacgag atcgaagtgg gccgctattc gatcgtttgc
     1501 cagacgacgc gcggccacgc ggcgccttcg caatcgatca gcgcgcgcgc ttgcgcgacg
     1561 gtgtcaccct ggcgcgcgcg cacccacacg aggaaatagc gactcttgac ggtatacaac
     1621 ggttcgccgc cgatcagcgt cgcggtctgc ggcaaacgcg cgtggaagtc ggtaatcgtg
     1681 cggaacggct gctgcgcacg gctcgcaacc agcgcagcaa gtgtcgccgc gtcgacgccg
     1741 tcgatcgacg cggccagcaa ttccgcggga gccgtgttga cgttgagcgg cgcgttctcc
     1801 ggcaacgccg tcacatagcg agtgagcgat gccagcacgc tcgtcgtgac gccgcgcacc
     1861 gcggcaagct cctcgacgcg catgacgggc atgttcgccg cgagccccgg ctgcggcgcg
     1921 ttgcggtacc acgcatcctc ggcgccatcg ggttcggcga ttgcgtcgct gtcgacccag
     1981 tcgacgatgg ccgcgagcgt cgccggcgcg acgtgcaatg catcgaaagc gcgtgcaaag
     2041 cgctggcgct cattcgcggg cgtgccagca agcgcgaggt tgttcacgtt gagcagccct
     2101 tgcgcatcga tgatgcggcc ttcgaccgag ccgttttcga ccggcgtcgc gggcagcggc
     2161 agcgcccatg gctcgccgag gtgattgaac gacgaggctc gcgtcatcgc gagtatctgc
     2221 cgcgtccact gtacgccggc gagcgcgatc gactgggcct gcacgagatc gcggcgttgc
     2281 gaaacgtgcg cggtccactg cgcctgc
//
LOCUS       DASSVX010000048         7646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_668234, whole genome shotgun sequence.
ACCESSION   DASSVX010000048 DASSVX010000000
VERSION     DASSVX010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668234"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..162
                     /locus_tag="VFO53_02220"
     CDS             7..162
                     /locus_tag="VFO53_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875119.1"
                     /translation="MKPGAACTEDDLRVHCLRMLGRYKSPRYLRVVNELPKGPSGKVQ
                     RLKLAEL"
     gene            174..950
                     /locus_tag="VFO53_02225"
     CDS             174..950
                     /locus_tag="VFO53_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET8875120.1"
                     /translation="MATPVEDHPRPHPPARGRNGVEPTFPVRIWRGSASGAFQQFDVP
                     RRASQTVLDVVTHVQRHLDPTLAYRYACRVGMCGSCAMSVNGKARWTCRTHVDAVANR
                     SGLEIAPLSHLPIVRDLVVDMAPFFDKWAAARGQFKPTATRHDEFAKVSPSSPARRAI
                     DATIECIGCGVCYASCDVVGWKPDYLGPAALNRAWTLVNDVRDGDGVARLRAVAAEGG
                     CQSCHTHQSCTRSCPKHLSPTASIAGLKRLVAQALVKGKL"
     gene            947..1330
                     /locus_tag="VFO53_02230"
     CDS             947..1330
                     /locus_tag="VFO53_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HET8875121.1"
                     /translation="MIDARTLAAPAEAAMRSARHAAWRFVLQRASAAVLALCVVVHLA
                     TIVYAVRHALTAEAIVNRMHASLLWPAFYALFVVAVAVHAPLGLRAVADEWLALRGRA
                     VDAVLALIALVLLGGGLYAVGSLAR"
     gene            1341..1721
                     /gene="sdhC"
                     /locus_tag="VFO53_02235"
     CDS             1341..1721
                     /gene="sdhC"
                     /locus_tag="VFO53_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HET8875122.1"
                     /translation="MSTTVSSTRAVRRNDLRARGHAGFLAFVVHRVSGIVLALFLPVH
                     FFALSQALSDPLALDGFLEWTRRPAVKFAETMLVLALAVHLAGGVRLLFVEFVGWRAE
                     WQKTAIALVAAVSLACALLFALNA"
     gene            1732..2613
                     /locus_tag="VFO53_02240"
     CDS             1732..2613
                     /locus_tag="VFO53_02240"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HET8875123.1"
                     /translation="MTLRIDPGRVEEAAKELYIRALKQLPPDIKQGFDTLVARETDRT
                     AQSVLGVMVKNIEVAEANDNLLCQDTGIPIYNVTIGRNVVVDGVALKAAIRRGCERAT
                     REHPLRSSVVHPLTRRNDHTSCGEGIPAIHVDFDDALDGLRIEMIPKGSGSENNSWLR
                     MAIPAEGVDAIKRFVVDCVLDAGGKTCPPTIVGVGVGGTSDLCVHLAKRAATRPLGTH
                     CADADGAALESELSRAVNLLGVGPQGLGGDATAFAVHVELAATHITMNPVAVNMQCHS
                     ARRASAVFTETGIAYGF"
     gene            2638..3294
                     /locus_tag="VFO53_02245"
     CDS             2638..3294
                     /locus_tag="VFO53_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875124.1"
                     /translation="MFLGHFGVALAARHVAPRPSLGTSIMAAQWADGIWPLFVLLGLE
                     HVEVSPGITRVTPLDFVSYPYTHSLLADGIWAVLFALVYGSLRRDWRGAGWLAVLVLS
                     HWVLDVVAHRPDMPTWPGGPVLGLGLWNSLPGTLIVEFALFASGAWLYARATRLRDTL
                     GRVLFASFCIALVVIYLASVFGPPPPGERTVAIAGVFGWLFVAWGYWIDRHRTPARAT
                     "
     gene            3314..3925
                     /locus_tag="VFO53_02250"
     CDS             3314..3925
                     /locus_tag="VFO53_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HET8875125.1"
                     /translation="MAHYELTMPVTEEAVRRLRINDTVTLQQRLFGIRDATLIALFDR
                     GRTTRLDLQGHAAIHTAPNVKKISASATNPAGYAPVCIGTTTSMRMERFTEPLLRELG
                     VRIVVGKGGLGASSLAAFRDLGGVYLAIIGGTAALETTWIEAIEDVDLDDLNPESLWQ
                     FRIRDFGPLLVAMDSHGASLYDAVDHAARDRRAAALAALNVKT"
     gene            3925..5652
                     /locus_tag="VFO53_02255"
     CDS             3925..5652
                     /locus_tag="VFO53_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET8875126.1"
                     /translation="MDIARLRTDVLILGAGGAGLFAALHAHQADPSLDITIAVKGLLG
                     KCGCTRMVQGGYNVALAPGDSIERHFMDTIEGGKWLPNQDLAWTLVNGAITRIHELEN
                     ELGCFFDRNPDGTIHQKAFAGQTFDRTVHKGDLTGIEIVSRLAEQVWARGIRRLEEHR
                     AIDFIAARDGDLAGVLLIDIRSGEPLFVQARAVLLATGGGPTMYRFHTPSGDKSCDGL
                     AMALRAGLPLRDMEMVQFHPTGLLAGVDTRMTGTVLEEGLRGAGGYLLNGDGERFMRD
                     YDPREERATRDIVSRAIFAEMRAGRTTPNGGVWISMAHLGPDNVRRQFKGMVERCADC
                     GFDLAGGRVEVVPTAHYMMGGVEFATDCTTGRDGLYVAGEDSGGVHGANRLGGNGVAN
                     STVFGGIAGDAIAQSLRRRAAWREPDADAVAASLDGARLPLAAGRGHGVDLETIRERL
                     YATMWDDAGIVRDAECLARAHTELHELRGELARYRLPETARDPSFNLTWHDWLNLANL
                     VAVSQAIVRAAQARENSRGAHFRADFAQPGDLASSTYTRVRDGGGGALTVEAVPVVFT
                     RVRPGESLV"
     gene            complement(5744..6070)
                     /locus_tag="VFO53_02260"
     CDS             complement(5744..6070)
                     /locus_tag="VFO53_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875127.1"
                     /translation="MISNSMRCTLFGGAVIALAGFTSLPVVAAALETSGAVEPVDGGR
                     VVIAQATGASVALAPRDVTPNDAFPAYQRGVREAAAQSNEALRRYIWRTRMIYDFYYP
                     DFASGE"
     gene            6391..7629
                     /locus_tag="VFO53_02265"
     CDS             6391..7629
                     /locus_tag="VFO53_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8875128.1"
                     /translation="MEGFNRRKALKTGLALGAAPLVAHPILTFGQGESPIKIGMHDPL
                     TGTYAAEGESEVRGAKLALAEINAKGGILGRKVDLVVEDDNANAGLAAQKAHKLIEKD
                     GCAMIMGAVSSATALSVNQVAAQKGVPYMVTGGHTDPVTGTECHWTTFRICTTTYMLA
                     NGLAATLHKKFGGKWYFITPDYAYGHTLQAAYAKILKGMGGEVLGNSLSPLGTTDFSS
                     YLIKAQSTNPQVLILLVDGQDLVNCMKQAVQFGLQKKMPIGGGLSELEVLNALSPEAR
                     IGWWTLEWYWNQPGTPHVKEWVDRYRKAYPDGSYPSARTWFGYAGLHSLALGAARAKS
                     IDPLKLAKGLEGLELPPEIKLQPNKVYFRPTDHQLMSSEFPGEILSDGKYPNMFKVAE
                     IVPGDKIALKDTGCKLDYSA"
BASE COUNT         1198 a         2572 c         2618 g         1258 t
ORIGIN      
        1 gtcgtcatga agccgggtgc cgcgtgtacc gaggacgacc tgcgcgtgca ctgcctgcgc
       61 atgctcggcc gctacaagtc gccacgctac ctgcgcgtcg tgaacgagtt gccgaagggt
      121 ccgtcgggca aggtgcagcg cctcaagctg gcggaactgt gacgactacc ttcatggcca
      181 cgcccgtcga ggatcacccg cggccgcacc cgccggcccg cgggcggaac ggcgtcgagc
      241 cgacgtttcc cgtgcgcatc tggcgcggat cggccagcgg cgcgttccag cagttcgacg
      301 tgccgcggcg ggcatcgcag acggtgctcg acgtggtgac gcacgtgcaa cgccacctcg
      361 atccgacact ggcgtatcgc tatgcatgcc gcgtcgggat gtgcggctcg tgcgcgatga
      421 gcgtgaacgg caaggcgcgc tggacctgcc gcacgcacgt cgacgccgtc gccaaccgca
      481 gtggcctcga gatcgccccg ctgtcgcacc tgccgatcgt gcgcgacctc gtcgtcgaca
      541 tggcgccgtt cttcgacaag tgggcggccg cgaggggaca attcaagccg accgccacgc
      601 gacacgacga gttcgcgaag gtctcgccgt cgtcgccggc ccgccgcgcg atcgacgcga
      661 cgatcgaatg catcggctgc ggcgtctgct acgcgtcgtg cgatgtcgtc ggatggaagc
      721 ccgattatct tggtcctgcc gcgctgaacc gcgcatggac actggtgaac gacgtgcgcg
      781 atggtgacgg tgtcgcgcgg ttgcgcgccg ttgccgcgga gggcggctgc cagtcgtgcc
      841 acacgcatca atcgtgcacg cgaagctgtc cgaagcatct gtcgccgacc gcgtcgatcg
      901 cggggctcaa gcggctggtg gcgcaggcgc tggtgaaagg gaagctgtga tcgatgcgcg
      961 aacgctcgcg gcgcccgccg aagccgcgat gcgcagcgcg cgccatgcgg cgtggcgctt
     1021 cgtgctgcag cgcgcgtcgg ccgccgtgct ggcgttgtgc gtcgtcgtcc atcttgcgac
     1081 gatcgtctat gcggtgcgcc acgcattgac cgccgaggcg atcgtcaacc gcatgcacgc
     1141 gagcctgctc tggcctgcct tctatgcgct tttcgtcgtt gccgtcgccg tgcatgcgcc
     1201 gctcggtctt cgcgccgttg ccgacgaatg gctggcgctg cgcggacgcg cggtcgatgc
     1261 cgttctggcg ctgatcgcgc tcgtgctgct cggcggcggt ctgtacgccg tcggctcgct
     1321 ggcccgctag gacgtccgcc atgtcgacaa ccgtttcgtc gacccgcgcg gtgcgccgca
     1381 acgacctgcg cgcgcgcgga cacgcgggct tcctcgcgtt tgtcgtgcat cgcgtttcgg
     1441 ggatcgttct cgcgcttttc ctgccggtgc atttcttcgc gctgtcgcag gcgctgtccg
     1501 atccgctggc gctcgacgga tttctcgaat ggacgcgccg gcccgcggtc aaattcgccg
     1561 agacgatgct cgtgctcgcg ctcgccgtgc atctcgccgg cggcgtacga ttgctgttcg
     1621 tcgagttcgt cggctggcgc gccgagtggc agaagacggc gatcgcactg gttgccgccg
     1681 tatcgctcgc gtgcgcgctg ctgttcgcgc tcaacgcatg aggagtgact gatgacgttg
     1741 cgcatcgatc cgggtcgtgt cgaggaagcg gcgaaagagc tctacatccg cgcgctcaag
     1801 cagctgccgc cggacatcaa gcagggcttc gacacgctcg tcgcgcgcga gaccgatcgc
     1861 acggcgcaat cggtgctcgg cgtgatggtg aagaacatcg aggttgccga agccaacgac
     1921 aacctgctct gccaggacac cggcattccg atctacaacg tcaccatcgg tcgcaatgtc
     1981 gtcgtcgatg gcgtcgcact caaggcggcg atcaggcgcg gctgcgaacg ggcgacgcgc
     2041 gagcatccgc tgcgctcgtc ggtcgtgcat ccgctgacac ggcgcaacga ccacacgtcg
     2101 tgcggcgaag gcattcccgc aattcacgtc gatttcgacg acgcactcga cggccttcgc
     2161 atcgagatga tcccgaaagg ctcggggtcc gagaacaatt catggctgcg gatggccatt
     2221 cccgccgaag gcgtcgacgc gatcaagcgc ttcgtcgtcg attgcgtgct cgacgcgggc
     2281 ggcaagacct gcccgccgac gatcgtcggt gtcggtgtcg gcggcacgtc cgacctgtgc
     2341 gtgcatcttg cgaagcgcgc cgcgacgcgt cctctcggca cgcattgcgc cgacgccgac
     2401 ggtgcggcac tggaaagcga gctgtcgaga gcggtcaacc tgctcggcgt cggcccgcag
     2461 ggcctcggcg gcgacgccac cgcattcgcc gtgcacgtcg agctcgcggc gacacacatc
     2521 acgatgaatc cggtcgccgt caacatgcag tgccattcgg cacgccgcgc gagcgcggtc
     2581 ttcaccgaaa ccggcatcgc gtacggcttc tgatcgcgca atcggcgccg ctcgcggatg
     2641 ttcctcggtc acttcggcgt ggcgcttgcc gcaaggcacg tcgctccgcg gccgtcgctc
     2701 ggcacgtcga tcatggccgc gcagtgggcc gacggcatct ggccgctgtt cgtgctgctc
     2761 ggtctcgagc acgttgaggt gtcgccgggc attacgcgcg tgactccgct cgattttgtt
     2821 tcgtatccgt acacgcacag cctgctcgcc gacggcatct gggcggtgct gttcgcgctc
     2881 gtctatggca gcctgcgccg cgactggcgc ggagcgggct ggctcgcggt gctggtgctg
     2941 tcgcactggg tgctcgacgt cgtagcgcac cggcccgaca tgccgacgtg gccgggtggg
     3001 ccggtgctcg ggctcggttt gtggaattcg cttcccggca cgttgatcgt cgaattcgcg
     3061 ctcttcgcta gcggcgcatg gctctacgca cgagcgacgc gcctgcgcga cacgctgggc
     3121 cgcgtcctgt tcgcatcatt ctgcatcgcg ctcgtggtca tctatctcgc gagcgtcttc
     3181 ggtccgccgc cgccgggcga acgtacggtg gcgatcgcgg gcgtcttcgg ctggctgttc
     3241 gtggcgtggg gatactggat cgaccgtcat cgcacgccgg cccgcgcgac gtgacacctt
     3301 cggaagatca gccatggccc attacgaact gacgatgccg gtgaccgaag aagccgtgcg
     3361 ccggcttcgc atcaacgaca cggtgacgct ccagcagcgg cttttcggca tccgtgacgc
     3421 aacgctgatc gcgctgttcg accgcggccg cacgacgcgg ctcgacctgc aggggcacgc
     3481 ggcgatccat acggcgccga acgtgaagaa gatttctgcc tcggccacga atccggccgg
     3541 ctacgcgccg gtgtgcatcg gcacgacgac gtcgatgcgg atggagcgct tcaccgagcc
     3601 gctgctgcgc gagctcggcg tgcgcatcgt cgtcggcaag ggcggactgg gcgcgtcgtc
     3661 gcttgccgca ttccgcgatc tcggcggtgt ctacctcgcg atcatcggcg gcacggcggc
     3721 gctcgagacg acgtggatcg aggcgatcga ggatgtcgac ctcgacgatc tcaatccgga
     3781 gagcctgtgg cagttccgca tccgtgactt cgggccgttg ctcgttgcca tggacagcca
     3841 cggcgccagc ctgtacgacg ccgtcgacca cgcggcacgc gaccgtcgcg ccgcggcgct
     3901 cgccgcattg aacgtcaaga cctgatggac atcgcgcggc ttcgtaccga cgtcctcatc
     3961 ctcggcgccg gcggcgcagg cttgttcgcc gcgctgcacg cgcatcaggc cgatccgtcg
     4021 ctcgacatca cgatcgcggt caagggactg ctcggcaagt gcggctgcac gcggatggtg
     4081 cagggcggct acaacgtcgc cctcgcgccc ggcgattcga tcgagcgcca cttcatggac
     4141 acgatcgagg gcggcaaatg gctgccgaac caggacctcg cctggacgct cgtcaacggc
     4201 gccatcacgc gcatccacga gctcgagaac gagctcgggt gctttttcga tcgcaatccc
     4261 gacggcacca tccatcagaa ggcgttcgcg ggccagacgt tcgaccgcac cgtgcacaag
     4321 ggcgacctca ccgggatcga aatcgtgagc cgcctcgccg agcaggtatg ggcgcgcggc
     4381 attcgccgcc tcgaggaaca tcgcgcgatc gacttcatcg cggcgcgcga cggcgacctt
     4441 gccggcgtgc tgctgatcga tatccgcagc ggcgagccgc ttttcgtgca ggccagggcg
     4501 gtgttgctcg ccaccggcgg tgggccgacg atgtatcgct tccatacgcc gtcgggcgac
     4561 aagagctgcg acggactcgc gatggcgctg cgcgcgggtc tgccgctgcg cgacatggag
     4621 atggtgcagt tccatccgac ggggctgctt gcgggcgtgg acacgcggat gaccggcacc
     4681 gtgctcgagg aggggctgcg cggcgcgggc ggctacctgc tcaacggcga tggcgagcga
     4741 ttcatgcgcg actacgatcc gcgcgaggag cgggcgacgc gcgacatcgt gtcgcgggca
     4801 atcttcgccg agatgcgcgc aggtcgcacc acgccgaatg gcggcgtgtg gatcagcatg
     4861 gcgcatctcg ggcccgacaa cgtgcgccgc cagttcaagg gcatggtcga gcgctgcgcc
     4921 gactgcggat tcgatctcgc cggcggccgg gtcgaggtcg tgccgaccgc gcactacatg
     4981 atgggcggcg tcgagttcgc gaccgactgc acgaccgggc gcgacgggct ctacgtggcc
     5041 ggcgaggatt cgggtggcgt gcatggcgcc aatcgcctcg gcggcaacgg cgtggccaac
     5101 tcgaccgtgt tcgggggcat cgccggcgat gcgatagcgc agtcgctgcg ccggcgtgcg
     5161 gcatggcgtg aaccggatgc ggacgccgtg gcggcaagcc tcgacggcgc gcggctgccg
     5221 ctcgcggccg gccgcggaca cggcgtcgac ctcgagacga tccgcgagcg gctctacgcg
     5281 acgatgtggg acgacgccgg catcgttcgc gatgccgagt gcctcgctcg cgcccatacc
     5341 gagctgcacg agttgcgcgg cgagctcgcg cgctaccggc tgcccgagac ggcacgcgac
     5401 ccgtcgttca acctgacgtg gcacgactgg ctcaatctcg cgaacctcgt ggccgtgtcg
     5461 caggcgatcg tgcgtgccgc acaggcgcgg gagaattcgc gcggcgcgca cttccgcgcg
     5521 gactttgcgc aacccggcga cctcgcctcg tcgacgtata cgcgcgttcg cgacggcggc
     5581 ggcggcgcgt tgacggtcga agcggtcccc gtcgtgttca cccgcgtacg gccgggcgag
     5641 tcgctggtct gagcggcggc aacgggcgag ggccattgcc cgatcgcccc cggccaaccg
     5701 gcgcgtcgcc ccggcccggc cggcggcggg aagcgcgtcg agtctattcg cccgacgcga
     5761 agtcggggta atagaagtcg tagatcattc gcgtgcgcca tatgtatcgg cgcagcgcct
     5821 cgttgctctg cgccgccgcc tcgcggacgc cgcgctgata cgcaggaaac gcgtcgttcg
     5881 gcgtgacgtc ccgtggagcc agtgcgacgc ttgcaccggt ggcctgcgcg atgaccaccc
     5941 ggccaccgtc gaccggctcg acggcgccgg acgtctccaa cgctgccgcg accaccggca
     6001 acgacgtaaa accggcaagc gcgatcactg cgccgccgaa cagcgtgcaa cgcatcgaat
     6061 tcgaaatcat tgccatctcc ttggtcggtg accccgggaa tccgcccgga cgaggaagcg
     6121 cctgtatccc gctatgcgcc atgcgcaagt tacgcctccg gcgccgagga tgtgcgcatt
     6181 gtgcaacgcc gccaaccgcg atgcgaccgg acacgggtac gggcgacgaa cctccggcag
     6241 gcgcgacgaa tgaacgcccg gcgtccacgg gccgcccggc gggtcgtggc gaaaatatct
     6301 cgtattacga tataatcgct gcacaagagc cgcggccagg tggaccgcgg ccgttcaggg
     6361 ccagtgcgtc atcaacatcc aggagggttc atggaaggtt tcaatcgccg caaggcgctg
     6421 aagaccgggc tcgcgctcgg tgcagcgccg ctcgttgccc acccgatcct gacgttcggg
     6481 cagggcgaat cgccgatcaa gatcggcatg cacgacccgc tgacgggcac ctacgccgcc
     6541 gaaggcgaga gcgaggtccg tggggcgaag ctcgcgctcg ccgaaatcaa cgccaagggc
     6601 ggcatcctcg gccgcaaggt cgacctggtg gtcgaggacg acaatgcgaa cgcgggcctc
     6661 gccgcgcaga aggcgcacaa gctgatcgag aaggacggct gcgcgatgat catgggcgcc
     6721 gtttcctcgg cgacggcgct gtcggtcaac caggttgccg cccagaaggg cgtcccctac
     6781 atggtcaccg gcggccatac cgacccggtc accggcaccg aatgccactg gacgacgttc
     6841 cggatctgca cgaccacgta catgctggcg aacgggctcg ccgccacgct gcacaagaag
     6901 ttcggcggca agtggtattt catcacgccc gactacgcgt acggacacac gctgcaggcg
     6961 gcgtatgcga agatcctgaa gggaatgggc ggcgaggtgc tcggcaattc gctgtcgccg
     7021 ctcggcacga ccgacttctc gtcgtacctg atcaaggcgc agtcgacgaa tccccaggtg
     7081 ctgatcctgc tggtcgacgg gcaggatctc gtcaactgca tgaagcaggc ggtgcagttc
     7141 ggactgcaga agaagatgcc gatcggcggc ggcctgtccg agctcgaggt gctgaacgcg
     7201 ctgtcacccg aggcgcgcat cggctggtgg acgctcgagt ggtactggaa ccagccgggc
     7261 acgccgcacg tcaaggaatg ggtcgaccgc tatcgcaagg cgtatccgga cggcagttat
     7321 ccgagtgcgc gcacctggtt cggctacgcc gggctgcatt cgctcgcgct cggggcggcg
     7381 cgcgccaaga gcatcgatcc cctcaagctc gcgaagggtc tcgagggtct cgaactgccg
     7441 ccggaaatca agctgcagcc gaacaaggtc tatttccggc cgaccgacca tcagctgatg
     7501 tcgagcgagt tcccgggcga gatcctgtcg gacggcaagt atccgaacat gttcaaggtc
     7561 gccgaaatcg tccccggcga caagatcgcg ctgaaggaca ctggctgcaa actcgactat
     7621 tcggcgtaga acgcgacaca tcttct
//
LOCUS       DASSVX010000049        20528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_484931, whole genome shotgun sequence.
ACCESSION   DASSVX010000049 DASSVX010000000
VERSION     DASSVX010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 20528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20528
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484931"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /gene="ilvB"
                     /locus_tag="VFO53_02270"
     CDS             complement(<1..1495)
                     /gene="ilvB"
                     /locus_tag="VFO53_02270"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HET8875129.1"
                     /translation="MELTGAEILIRCLQEEGVEYVFGYPGGAVLNIYDELFKQTRVKH
                     VLVRHEQGAVHAADGYSRSSRKVGVALVTSGPGVTNAVTGIATAYMDSVPMVVITGQV
                     PTAAIGQDAFQECDTVGITRPCVKHNFLVKNVADIAVTMKKAFYLATTGRPGPVLVDI
                     PKDTSQAKTEFAYPKTVSLRSYNPVTKGHAGQIRKAVQLLLEAKRPMIYAGGGVILND
                     ASEALTRLVRLLGFPCTNTLMGLGGFPGTDPQFTGMLGMHGTYESNMAMQHCDVLLAV
                     GARFDDRVIGDPAHFYRADRKIIHIDIDPSSISKRVKVDVPIVGYVGDVLSEMVKLVE
                     SSPARPDAAALKAWWAEIKEWQRKDCLRYDRASKLIKPQFVLEKLYELTRGDAFVTSD
                     VGQHQMWAAQYYKFDKPRRWINSGGLGTMGFGLPAAMGVQLVNPGATVACVTGEASIQ
                     MCIQELSTCKQYHLPIKIVNLNNRYMGMVRQWQEFFYGNRYAESYMDA"
     gene            1859..2428
                     /locus_tag="VFO53_02275"
     CDS             1859..2428
                     /locus_tag="VFO53_02275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET8875130.1"
                     /translation="MAQPQEISSFLAEVERRAFKQAMYAVRDEDAALDIVQDAMLKLT
                     ENYAGKPAVELPMLFQRILQNTIHDHFRRQKVRSTWTTLLSALGHKDDKDDDYDPLET
                     LQAKTDSNAPADPAAHAEQRQTMRIIEQALSRLPARQREAFLLRYWEELDVAETAAAM
                     GCSEGSVKTHCSRAVHALADVLKAKGMTL"
     gene            2425..2823
                     /locus_tag="VFO53_02280"
     CDS             2425..2823
                     /locus_tag="VFO53_02280"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HET8875131.1"
                     /translation="MNDREFEFGRKIARCLDQGIADLRPGTVYRLQQARGAALAAASS
                     AHRTEPELATAGAHGGSAGRTSLRNGSLRWLGLALLVAAAGFGYQQWHSIAQVREFED
                     LDMHLLASDLPIDAYLDRGFQNWLRNGLER"
     gene            2796..3314
                     /locus_tag="VFO53_02285"
     CDS             2796..3314
                     /locus_tag="VFO53_02285"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HET8875132.1"
                     /translation="MASERVRALTRAVAVLALAASAANAQIALKSPSWNELSEHDRQV
                     LSPLASDWNNLDAQRKTKWLGLAKRYATLPPEQQQRIQGQMREWSRLTPDQRNAARER
                     YKSLKSLPPDKKNEVRRKWQEYQSLPPEKRNELATKPGTPPRPGPRSPSGASAVAAPP
                     RIPATPPRPPGQ"
     gene            3311..3820
                     /locus_tag="VFO53_02290"
     CDS             3311..3820
                     /locus_tag="VFO53_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004526453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET8875133.1"
                     /translation="MTALARPSLASAGRRLAALGYEALLLVAMAFVAGFVFLPFLPQD
                     ASRHTLTVASPLARAAMFFALVAAAAVYCTWCWSRGRRTLPQKTWRLRIVDRRGEPLG
                     ARHALARYAAWWIGPGVALAAYALLQPLGYGRNALAFAVLNYCWAIVDPDRQFLHDRI
                     AGTALVQDP"
     gene            3841..4227
                     /locus_tag="VFO53_02295"
     CDS             3841..4227
                     /locus_tag="VFO53_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET8875134.1"
                     /translation="MTTRDRDREAILQAFADPARLVVVSLCAAWCDTCTQFRGAFERL
                     AQARPHAAFVWLDIEDDAAIAGDVDVENFPTLAIYRGRSPLHFGVALPHETTVGRLVD
                     ALAEGAPARTDMPDEVVELPSRFTPV"
     gene            complement(4241..5137)
                     /locus_tag="VFO53_02300"
     CDS             complement(4241..5137)
                     /locus_tag="VFO53_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8875135.1"
                     /translation="MLWSTGFISAKYGLPYAPPLKFLLVRFALAAALMTVVTLATRAP
                     WPRGVRQVGHVAVAAALVHGVYLGGVFVSIAGGMPAGTSAMLVGLQPILTVVLARVWL
                     GERISARQWLGLGLGLAGVYLVVRHKIALDADALTLAPIVAALAGISIGTLYQKRFVA
                     HVDLRSGAVVQFAACVLLYAPIVAWLEPAPVAWTPQFAFALGWSVIVLSAGAISLLYW
                     LLRHGAAADVARLFYLVPPVTALMAFVLFGERLDAFALGGMVLIALGVALARRKQNRS
                     GVRDDFRRSPPGMPTLPPESRL"
     gene            complement(5219..5767)
                     /gene="ppa"
                     /locus_tag="VFO53_02305"
     CDS             complement(5219..5767)
                     /gene="ppa"
                     /locus_tag="VFO53_02305"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET8875136.1"
                     /translation="MSLDRVPAGFDVPNDCNVVVEIPMHGEPIKYEVDKETGAVFVDR
                     FMSTSMHYPCNYGYIPQTLSDDGDPCDVLVLSPVPLITGVVVRCRPIGMLRMDDEAGG
                     DAKILAVPIERLSSLYRAINAPDDLPGIQLAQIAHFFEHYKDLEAGKWVRLAGWAGPD
                     DAKREISESVDRFRAKAPGAEA"
     gene            5857..6627
                     /locus_tag="VFO53_02310"
     CDS             5857..6627
                     /locus_tag="VFO53_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HET8875137.1"
                     /translation="MPRERAQLYRRMLHLALAASLIAPASSADADAQAAHPAQPPPFS
                     AMHAGRALAPWTLVSIGIGKRPTVYDLVDDAGTIVLHAAADNAASALAYPLHAELRDA
                     PVVSWRWKIAGVIPGADPHVASREDAPARLVLEFDGDRSRLPLLDRGIYGLAKRAAGR
                     ELPYATLMYIWSNRDRIGTVIANPHTRRIQMIVASSGGAGAGQWQSLARNARADFLRA
                     FGEEPGTLTAVGVLTDSDNTDGHAEAWYGDIRFEPAPH"
     gene            6647..7342
                     /locus_tag="VFO53_02315"
     CDS             6647..7342
                     /locus_tag="VFO53_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8875138.1"
                     /translation="MSAVRTLSPMQSLQPTPTIVEQVYRTILDAICDGRLPPGERLTQ
                     ESVAAKLAVSRQPVGQALLLLKQQKFVSEAGRRGLMVAPLDRDFMRSIYELRLGIEPL
                     AATLASRRANGSDVARGEAIIAAGKAAVRDNSITDLIASDMRFHMLLYELSGNRLFVD
                     LMEELWNHLRRAMREVLQHREYRKAIWVEHEQILRAVTKRDGDAAAALVRAHLEHAAV
                     NVQVVLPTAETTA"
     gene            complement(7348..8436)
                     /locus_tag="VFO53_02320"
     CDS             complement(7348..8436)
                     /locus_tag="VFO53_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET8875139.1"
                     /translation="MVDGLILDYLRHAPAIGGELRVAKHGALIGRSTIGARSTLGDYA
                     TVRADGEDVRIGAGAWFGDHATVHIADRIRGAVIGDDVTVGRYGVAHACTLGDGVVIG
                     ESSIVMDDAGIGAHAVIAGDSVVPPRKMLAGGFLYAGHPAKPVREITVAEAQSLARDL
                     RSGRPAAELRSRHVPAWTDAVRSLPKANDGPLRALHGRSPRVTNAYVAATAIVAGDVE
                     MGDDASIFFGCAVSAGDGRIVIGARSNVQDNCVLATDRRRGDLVLGVGVTIGHNVELG
                     SGIIGDDALVGMGSRVGDHVVVEHGGCIGAGAWVEPHTVVKAQWVWAGRPARAFRPLK
                     PEERAEFARARDIYVTYSTDYRRGNATG"
     gene            complement(8441..10141)
                     /locus_tag="VFO53_02325"
     CDS             complement(8441..10141)
                     /locus_tag="VFO53_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET8875140.1"
                     /translation="MNDSTILADMRARAALAADVDAWEKNEVAKYVARTPERTDDFQT
                     VGGFPLKRTYTALDVADTPLDDIGLPGRYPFTRGPYPTMYRGRLWTMRQIAGFGTAED
                     TNQRFKYLIAHGQTGLSTDFDMPTLMGYDSDHPMSEGEVGREGVAIDTLADIEHLFDG
                     IDLTKISVSMTINPSAWILLAMYVALAKKRGYDLHALSGTIQADILKEYQAQKEWAFP
                     VAPSVRIVRDCITYCARNMKRYNPINISGYHMSEAGASPLDEVAFTMCNLIAYVEEVL
                     RTGMQVDEFAPRLAFFYVCQADFFEEIAKFRAVRRVYAKIMHDRFGAKNPESMRLRFH
                     CQTAAASLTKPQYPINVVRTGLQALAAVLGGAQSLHTNGMDEAFAIPTEEAMKIALRT
                     QQIIAHESGVADVVDPLGGSYFVESLTTQYERAIFGVIDEVDRGGGTVRLTQEGWFQR
                     RIADFAYETALKKASGEKPVIGVNRFVDPEEKLAIELHPYDASTAQRQIARLQRVRRE
                     RDNAKVSALLERLVEVAKRQDENIMPVTIELVSAGATMGDIIEKLKTLWGTYRENPVF
                     "
     gene            complement(10138..11199)
                     /gene="meaB"
                     /locus_tag="VFO53_02330"
     CDS             complement(10138..11199)
                     /gene="meaB"
                     /locus_tag="VFO53_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET8875141.1"
                     /translation="MAYIRSADLESRVLAGDTRAIARLLSRAESGIAEARPTLDAMFA
                     HAGRAQVVGITGVPGSGKSTLVAKLVPEIRKSGRKVAVVAVDPSSPFSGGAILGDRIR
                     MGDLAGDAGVFVRSMATRGALGGLAKGTLEAVDVLDAAGFDMVLIETVGVGQDEVDVA
                     RAAHTTVVVSAPGLGDDIQAIKAGILEIADIHVVSKCDRPDANRTIADLKTMLALGYT
                     HMDTAQLKSGSELYLPDGSSRAADAHAGRYSSDPDFTLPAVIGTSAIQDRGVAELLVA
                     IDRHRETLERTGEIEARRRAIAERRLLAAGEGLLHDAFARHRLGKLSPLLERLRVRSL
                     SPHAAARQLLRELNIGGDA"
     gene            complement(11203..12627)
                     /locus_tag="VFO53_02335"
     CDS             complement(11203..12627)
                     /locus_tag="VFO53_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HET8875142.1"
                     /translation="MEQWSFPPRFDAHYVPPVDSRYWFPVRETMPPGDRERAILERLK
                     AVTRYAYDTSPFYRRKWDDAGFHPGHLRSLEDFEAKVPVITKQDLRDAQARVPPFGDY
                     LCVPGAEVHHIHGTSGTTGRPTAFAIGRADWDAIANAHARILWGMGIRPGDVVFVAAI
                     FSLYMGSWGALAGAERLRAKAFPFGAGAPGMTARAAQWLDLVKPAALYATPSFALHLA
                     EVARDEGHDPRTFGLSTTFFSGEPGASVPGVRDRLIAAYGARVVDCGSMAEMTPFMHV
                     AGSAESAGMLCWQDIVYTEVCDPATYRRVPYGQRGTPVYTHLERTSQPMIRLASGDLT
                     LWDHGPTPCGRTYPRLPQGIFGRIDDMFTIRGENVYPSEVDAVLSQLHGYGGEHRIVI
                     TRDGAMDELVIRIEIAPEQHAQGAAAHSALRENAERRLSKMLGLRSRLEVVPPGTFPR
                     TDFKARRVVDDREVFRELNARLRS"
     gene            complement(12679..13095)
                     /locus_tag="VFO53_02340"
     CDS             complement(12679..13095)
                     /locus_tag="VFO53_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009564903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET8875143.1"
                     /translation="MEQSTQPIRVVIAKVGLDGHDRGVKIVARTLRDAGMDVIYTGLH
                     RTPDEVVEAAIQEDVDVIGVSILSGAHMTVFPRLIGLLKERDAEDILVVGGGVIPDED
                     LPKLKAIGVRELLLQDTPPRQIVETIRRLVAERGPR"
     gene            13247..14197
                     /locus_tag="VFO53_02345"
     CDS             13247..14197
                     /locus_tag="VFO53_02345"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HET8875144.1"
                     /translation="MPRTSLPWLVLATIVVSAALAWWTLTRTVGGEPAARLEHRDLAP
                     FHEIEVGGSAAVTLLQAEAESIDVEAASRTSVAAEVANGRLVVHARDRRRWWNGLLGR
                     RSSATPHVTVRFRTLDAIALTGNVKMDAPKLSTTALRIDASGGSALSIDDLRATSLRV
                     NGSGALDAKLAGRVEREDVSISGAGSYRAEHLVATDATVSVSGVGNVIVHATSTLGAS
                     ISGAGVIEYAGNPAVTEHVSGIGRVKRRAPEQRSGLTTVAIHAQCSGVSADAPWSLKN
                     SGPPVTGSMSAWTPGMQRGADTRQSRRSAMSIAATSCTVS"
     gene            15298..17010
                     /locus_tag="VFO53_02350"
     CDS             15298..17010
                     /locus_tag="VFO53_02350"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET8875145.1"
                     /translation="MRVSKFYLSTLKEAPAEAEVASQKLMLRAGLIKKVSSGIYSWMP
                     LGLITLRKVEQVVREEMNRAGALETYLPHILPAELWEETGRWDKFGPLLLKIKDRQAR
                     DFLFGPTHEEVMTDIVRKDIRSYRQLPVNLYQIQVKFRDEIRPRFGVMRGREFLMKDA
                     YSFDADRDALMVSYRGMYDAYARIFTRLGLEFRAVEADTGAIGGFASHEFQVLADSGE
                     DAIAYSNGSTYAANVEQAEAIAPSTPRAAPSEAMQRVPTPGSSTCEQVAELLGLPLAR
                     TVKCLLIHAQDRVQMLLVRGDHMGNEVKIGKLPGLDGWRFASEAEVVAATGCRPGYLG
                     PVGIAGDMPLIVDRQVAAMSDFVCGANEAGYHLRGVNFGRDCREPDAVADIRNVVEGD
                     PSPDGRGTLSIVRGIEVGHVFALGDVYSTAMGASYLDADGRSQVLQMGCYGIGVTRVV
                     AAAIEQNHDERGIVWPSAMAPFAVAIAPIGYDRSDAVRECADRLHRQLAEAGVEVLLD
                     DRGERPGVMFADLELIGIPHRVTVGERGLKEGIVEYQARRDATVTRLPLADTLAFLNE
                     RIGT"
     gene            17043..17666
                     /locus_tag="VFO53_02355"
     CDS             17043..17666
                     /locus_tag="VFO53_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET8875146.1"
                     /translation="MFAALAFLAACAAPLDAHAGAQVEERLAPSVASGLSRAVADNPV
                     PRNYIERAAVREWLAAMSPKLAGRFPDEAARVEFLATVHYEATRAGLDPQLVLGVIHH
                     ESNFRKYAVSSADARGFMQVMPFWVRLIGTADQNLFQLRTNLRYGCTILRHYLDVENG
                     DVYRALGRYNGSLGQPEYPTAVMTAMKRYEPGTSVAAVRTSATASLH"
     gene            17663..18604
                     /gene="gluQRS"
                     /locus_tag="VFO53_02360"
     CDS             17663..18604
                     /gene="gluQRS"
                     /locus_tag="VFO53_02360"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HET8875147.1"
                     /translation="MSASGPPEGPYRGRFAPSPTGPLHFGSLVAAMASYADARRNAGA
                     WLVRIEDVDVPRARPRAADDILHALEGYGFEWEGPVWRQSSRRDAYEAALARLAAAGL
                     AYACVCTRQALAAQPIGRIGERVYPGTCRDGIGHHGDRAAERTRRAAVRVRVPSARIA
                     FTDRLHGLQQQRLDEDVGDFVVRRSDGLCAYQLAVVVDDAAQRITDVVRGADLLASTP
                     RQIFLQRALGVATPRYLHVPVAVDARGAKLSKQTAATPLPAAPLPALHAAWRFLGQCA
                     PSDPACPVSEFWTWAIAHWDAGRIPAAATRPAPTAIV"
     gene            18669..19241
                     /locus_tag="VFO53_02365"
     CDS             18669..19241
                     /locus_tag="VFO53_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8875148.1"
                     /translation="MTTLVVVRKGDSVAIAADSLTTFGDTRLAAEHDRTYDKIVRYRD
                     TYIGLCGSAAHQLVFESLLAKHGDLDFAHKSAIFETFRKLHPILKEQHFLNPKEEEDD
                     PYESTQITALIANANGIFGVFSMREVFEYTQFWAAGSGREFALGAMHARYTQTKTAET
                     VARIGIEAGAKFDRNSALPMTLYTLPLTKQ"
     gene            19238..20140
                     /locus_tag="VFO53_02370"
     CDS             19238..20140
                     /locus_tag="VFO53_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET8875149.1"
                     /translation="MSPPRVADALVPFVVEHAPVRGSLIRLEATSRDIVAQHDYPPPL
                     ARVLSELCAAAALLASTLKLDGSLVVQLAGDGPVRLLVVECTDALELRATAQWDRRRV
                     AALGENASLAALSGGAGHGRLTLTLDQRGAGTLYQGVVRLDAASVAASIEHYLAASEQ
                     LQSRLWLLPRDAGVTGLLLQRLPDASAGDFTGWPRLVDDAGRAIARAVRGATLEAILA
                     GLFPRNDIRMFEPRPARFRCRCSTDRVAHALRIAGHAEIEAALAARGMVEVTCEFCNR
                     RYTFGPDEARALFAQAPDPGATST"
     gene            20137..>20528
                     /locus_tag="VFO53_02375"
     CDS             20137..>20528
                     /locus_tag="VFO53_02375"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HET8875150.1"
                     /translation="MSVYTPSTFAITDRAAIARLMHDHPFATLVTPATPEPFISHLPL
                     LFVPGCEPHGTLIGHMARANPHWEHAANVESIAIFHGPHAYVSPSWYAEPAKAVPTWN
                     YAAAHVHGTLEVIRDPLETRRVLDALVQR"
BASE COUNT         3303 a         7105 c         6871 g         3249 t
ORIGIN      
        1 gcgcgtccat gtacgactcg gcgtagcggt tgccgtagaa gaattcctgc cactgccgca
       61 ccatccccat gtagcgattg ttgaggttca cgatctttat cggcaggtga tactgcttgc
      121 aggttgacag ctcctggatg cacatctgga tcgacgcctc gccggtgacg caggcaaccg
      181 tcgcaccggg attgacgagt tgcacgccca tcgccgccgg cagtccgaat cccatcgtgc
      241 cgagtccgcc cgaattgatc cagcgccgcg gcttgtcgaa cttgtagtac tgcgcggccc
      301 acatctggtg ctgaccgacg tcggaggtga cgaacgcgtc gccgcgcgtc aactcgtaca
      361 gcttctcgag cacgaactgc ggcttgatga gcttgctcgc gcggtcgtag cgcaggcaat
      421 ccttacgctg ccattccttg atctcggccc accatgcctt cagcgccgcg gcgtcggggc
      481 gcgcgggcga cgattcgacg agcttcacca tctcggacag cacgtcgccc acgtagccga
      541 cgatcggcac gtcgaccttc acccgtttgc tgatcgacga cggatcgatg tcgatgtgga
      601 tgatcttgcg gtcggcgcga tagaagtggg ccggatcgcc gatgacgcgg tcgtcgaagc
      661 gcgcgccgac cgcgaggagc acgtcgcagt gctgcatcgc catgttcgat tcgtacgtgc
      721 cgtgcatgcc gagcatgccc gtgaactgcg ggtcggtgcc cggaaagccg ccgaggccca
      781 tcagcgtgtt cgtgcacgga aagcccagca ggcgcaccag gcgtgtcagc gcctcggacg
      841 catcgttcag gatcacgccg ccgccggcat agatcatcgg ccgcttcgcc tcgagcagca
      901 gctgcaccgc cttgcggatc tggccggcgt ggcccttggt caccgggttg tacgagcgca
      961 gcgacaccgt tttcggatac gcgaattcgg tcttcgcctg cgaagtatcc ttgggaatgt
     1021 cgacgaggac cgggccgggg cggccggtgg tcgcaagata gaacgccttt ttcatcgtga
     1081 ccgcgatgtc ggcgacgttc ttgacgagga aattgtgctt cacgcacgga cgcgtgatgc
     1141 cgaccgtgtc gcattcctgg aacgcgtcct gcccgatcgc ggcggtgggc acctgtccgg
     1201 tgatcacgac catcggcacg gagtccatgt aagcggtcgc gatgccggtc accgcgttcg
     1261 tgacgcccgg cccggaggtg acgagcgcga cgcccacctt ccgcgacgag cgcgaatagc
     1321 cgtccgccgc atgcacggcg ccctgctcgt ggcgcaccag cacgtgcttg acgcgcgtct
     1381 gcttgaacag ctcgtcgtag atgttgagga ccgccccgcc ggggtagccg aagacgtact
     1441 cgacgccttc ttcctgaaga cagcggatca ggatttccgc gcccgtcaat tccatgagtc
     1501 catgcctcgc gtatgccgtc gcgccatcgc aaccgaacgt cgaatcggtc gcactcgtca
     1561 aatcggtcgc actcgcgtgg agcgcgcgcc cgccaaggct cgatcgggta gaaagccgca
     1621 aattgggcag cgggccgtct ccagggaggc tcgttggcgc tcgtgacgcc gcggagggag
     1681 agttcatcgg cccttgttgc aatacagcat ccgggcctcg cggtccggag agcaagacag
     1741 taccggcaac ggttggcgtg gtcaagaccg gattgctacg gcgcagcatg gaagcgtggt
     1801 gcgggacccg cacgccgaac gcctataatc gccgccgccc ccaaaggaat cgtcggaact
     1861 ggcccagcca caggaaattt cgagctttct cgccgaagtc gagcggcgcg ccttcaagca
     1921 ggcgatgtac gccgttcgcg acgaggatgc tgccctcgat atcgtgcagg acgcgatgct
     1981 gaagctgacc gagaattacg cgggcaagcc ggccgtcgag ctgccgatgc tgttccagcg
     2041 catcctgcag aacacgatcc acgatcattt ccgtcgccag aaggtgcgct cgacgtggac
     2101 gacgctcctg tcggcgctcg gtcacaagga cgacaaggac gacgattacg atcccctgga
     2161 aacgctgcag gcgaaaaccg actcgaacgc cccggccgac ccggcggcgc acgccgaaca
     2221 gcggcagacg atgcgcatca tcgagcaggc gctgtcgcgg ctcccggcgc gtcaacgcga
     2281 ggcgttcctg ttgcgttact gggaggaact cgatgtcgcg gagaccgccg ctgccatggg
     2341 atgctccgaa ggcagcgtga aaacgcattg ctcccgcgcc gtccatgcct tggcagacgt
     2401 tctcaaggca aagggaatga cgttatgaac gatcgggaat ttgaattcgg ccggaagatt
     2461 gcgcgctgcc tcgaccaggg cattgccgat cttcggccgg gaaccgtcta tcgcctgcag
     2521 caggcgcggg gcgcggcgct ggccgcggct tcctcggcgc accgcaccga gcccgaactg
     2581 gccacggcgg gcgcgcacgg cggctcggca ggccgcacga gcctgcgcaa cggctcgtta
     2641 cgctggctgg ggctcgcgct gctcgtggcc gccgccggct tcggttacca gcagtggcac
     2701 tcgatcgcgc aggtccgcga attcgaggac ctggacatgc atctgctcgc gtcggatctt
     2761 cccatcgacg cctatctgga caggggattc cagaattggc ttcggaacgg gttagagcgc
     2821 tgacgcgcgc ggtcgccgtc ctcgcgctcg ccgcctccgc ggccaacgcg cagatcgcgc
     2881 tcaagtcgcc gtcctggaac gagctgtccg agcacgatcg ccaggtgctc tcgccgctcg
     2941 catccgactg gaacaacctc gatgcgcagc gcaagacgaa atggctgggt ctcgcgaagc
     3001 gctacgcgac gcttccgccc gagcagcagc agcgcatcca gggtcagatg cgcgaatggt
     3061 cgcgcctgac acccgaccag cgcaacgccg cgcgcgagcg ctacaagtcg ctgaagtcgc
     3121 tgccgccgga caagaagaac gaggtgcgcc gcaagtggca ggagtaccaa agcctgcccc
     3181 ccgagaagcg gaacgagctt gcgacgaagc cgggcacgcc accgcggccc ggtccgcggt
     3241 ccccgtccgg tgcctcggcc gtggcagcgc caccccgcat tccggccacg ccgccacgcc
     3301 cgcccgggca gtgacggcgc tggcccggcc atcgcttgca tcggctgggc ggcgtctcgc
     3361 ggcgctcggc tacgaagcgc tgttgctggt cgcgatggcc ttcgtcgccg ggttcgtctt
     3421 cctgccgttc ctgcctcagg atgcgagccg gcacacgctg acggtcgcat cgccgcttgc
     3481 acgcgcggcg atgttcttcg cgctcgtcgc cgccgccgcc gtctactgca catggtgctg
     3541 gagtcgcggc cggcgcacgc tgccgcagaa aacgtggcgg ctgcgcatcg tcgatcggcg
     3601 cggcgaaccg ctcggtgccc gccacgcgct cgcccgctat gccgcctggt ggatcggacc
     3661 gggcgtggcc ctcgccgcct acgccctgct ccagcccctc ggctacgggc ggaacgcgct
     3721 cgcgttcgcc gtgctcaatt actgctgggc gatcgtcgat cccgaccgcc agttcctgca
     3781 tgaccggatc gcgggcacgg cgctggtaca ggacccatga caagcgatgc gatgatggta
     3841 ttgacgacgc gagatcgcga ccgcgaagcg atcctgcagg cgtttgccga tcccgcacgc
     3901 ctcgtcgtcg tgtcgctgtg cgcggcctgg tgcgacacct gcacgcagtt tcgcggcgcg
     3961 ttcgagcggc ttgcccaggc gcgtccgcat gcagcgttcg tgtggctcga catcgaggac
     4021 gacgcggcga tcgccggcga cgtcgacgtc gagaacttcc cgacgcttgc gatctatcgc
     4081 ggccgctccc cgctgcattt cggcgtcgcg ctgccgcacg agaccaccgt cggccggctg
     4141 gtcgacgcgc tcgccgaggg tgcgcccgcg cgcaccgaca tgcctgacga ggttgtcgaa
     4201 ttgccgtcgc gattcacacc cgtctagcat cgaaataggg tcagagacga ctttcgggtg
     4261 gcagcgtcgg cattcccggc gggcttcgcc gaaagtcgtc tctgaccccg cttcggtttt
     4321 gcttgcgccg tgccagtgcg acgccgagcg cgatcagcac catcccgccg agcgcgaacg
     4381 cgtcgagacg ctcgccgaac agcacgaacg ccatcagcgc ggtgactggc gggaccagat
     4441 agaagagccg cgcgacatcc gccgcggcgc cgtgacgcag cagccaatag aggaggctga
     4501 tcgcaccggc cgaaagcacg atcaccgacc agccgagcgc gaacgcaaac tgcggcgtcc
     4561 atgcaaccgg cgcaggctcg agccaggcga cgatcggcgc atacagcagc acgcaggccg
     4621 cgaactgcac gacggcgccg ctgcgcaggt cgacatgcgc gacgaaccgt ttctggtata
     4681 gcgtgccgat gcttatgccg gcgagcgctg cgacgatggg agcgagcgtc agcgcatcgg
     4741 cgtcgagggc gatcttgtgg cgcacgacga ggtacacgcc ggcgaggccg aggccgaggc
     4801 caagccactg ccgcgcgctg atgcgctcgc cgagccatac gcgcgcgagg acgacggtga
     4861 ggatcggttg cagtccgacg agcatggcgc tcgttcccgc cggcatgccg ccggcgatgg
     4921 agacgaacac cccgccgagg tacacaccgt gcacgagcgc cgccgcgacg gcgacgtggc
     4981 ccacctgacg cacaccgcgc ggccacggtg cacgcgtggc gagcgtcacg accgtcatca
     5041 acgcggcggc gagcgcgaag cggacgagca ggaacttgag cggcggcgca tagggcaggc
     5101 catacttggc cgagatgaag ccggtgctcc agagcacgac gaaaagcgcc gggagcagcg
     5161 ggttggcgcg cgcgcccagg ctaccgcctg tccggccggc cgccgcgcgc aacctgcatc
     5221 acgcctcggc acccggcgcc ttcgcgcgga agcgatcgac gctttcgctg atctcgcgct
     5281 tcgcgtcgtc gggtccggcc cagcccgcaa gccgcaccca tttccccgcc tcgagatcct
     5341 tgtagtgctc gaagaaatgc gcgatctgcg cgagctgaat cccgggcagg tcatccggcg
     5401 cgttgattgc gcggtacagc gacgacagcc gctcgatcgg caccgcgagg atcttcgcgt
     5461 cgcctccggc ctcgtcgtcc atcctcagca tgccgatcgg ccggcagcga acgacgacgc
     5521 cggtgatgag cggcaccggc gacagcacca gcacgtcgca aggatcgccg tcgtcggaaa
     5581 gcgtctgcgg aatgtatccg tagttgcacg ggtagtgcat cgacgtcgac atgaagcgat
     5641 cgacgaacac ggcgcccgtt tccttgtcca cctcgtactt gatcggctcg ccatgcatcg
     5701 ggatctcgac gacgacgttg cagtcgttcg gcacgtcgaa gccggcaggg acgcgatcga
     5761 ggctcacgca atggctttca tgcttcgctc ggctaccggc gccggcggcg gcgccttgcg
     5821 ctgatgacag acgcggcagg gcgacagatg aaaacgatgc cgcgggaaag ggcgcaatta
     5881 taccggcgca tgctgcacct ggcgctggcc gccagcctga tcgcgccagc gtcgagcgcc
     5941 gatgccgacg cacaggccgc gcatcctgca caaccgccgc ctttctcggc gatgcatgcc
     6001 ggccgcgcgc tggcaccgtg gaccctggtg agcatcggca tcggcaagcg tcccacggtt
     6061 tacgacctcg tcgacgacgc cggcaccatc gtgctgcacg cggccgccga caacgcggcg
     6121 agcgccctcg cctatccgct gcacgccgaa ctgcgcgatg cgccggtcgt ttcgtggcgc
     6181 tggaagatcg ccggtgtgat tcccggcgcc gacccgcacg tggcgtcgcg cgaagatgcg
     6241 ccggcgcggc tggtgctgga attcgacggc gacagaagcc ggctgccgct gctcgaccgc
     6301 ggtatctacg gcctcgcgaa gcgtgccgcc ggccgcgaac tgccgtatgc gacgctgatg
     6361 tacatctggt cgaaccgcga ccgcatcggc acggtgattg caaacccgca cacgcgacgc
     6421 atccagatga tcgtcgcgtc gagtggcggc gccggtgcgg gtcagtggca gtcgcttgcg
     6481 cgaaacgccc gggccgattt cctgcgtgcc ttcggcgagg agccgggaac cctgaccgcc
     6541 gtgggcgtgc tgaccgacag cgacaacacc gacggccacg ccgaagcgtg gtacggcgac
     6601 atccgcttcg agccggcgcc gcattgaatg cggctatgat cgcaagatga gcgcagtgcg
     6661 cacgctgtcg ccgatgcaat cgctgcagcc cacgccgacg atcgtcgagc aggtctatcg
     6721 cacgatcctc gacgcgatct gcgacggacg gctgccgccc ggcgagcgcc tcacgcagga
     6781 gtccgtggcc gcgaagctcg ccgtgtcgcg gcaaccggtg ggccaggcgc tgctgctcct
     6841 caagcagcag aagttcgtca gcgaagccgg acgccgcggc ctgatggtgg cgccgctcga
     6901 ccgcgatttc atgcgctcga tctacgaatt gcgcctcggc atcgagccgc tcgcggcaac
     6961 gctcgcgtca cggcgggcga acggcagcga cgtcgcgcgc ggcgaggcga tcatcgccgc
     7021 gggcaaggcc gccgtgcgcg acaactcgat caccgatctc atcgccagcg acatgcgctt
     7081 ccacatgctc ctgtacgagc tctcgggcaa ccggctgttc gtcgatctca tggaggagct
     7141 gtggaatcat ctgcgccgcg cgatgcgcga ggtgctgcag caccgcgagt atcgcaaggc
     7201 gatctgggtc gagcacgagc agatcctgcg cgccgtgacg aagcgcgacg gcgacgccgc
     7261 cgcagcactt gtccgtgcgc acctcgagca cgcggccgtc aacgtgcagg tggtgctgcc
     7321 gacggcggaa acgaccgcgt agtcgcgcta gccggtcgcg ttgccgcggc ggtaatcggt
     7381 cgagtacgtc acgtagatgt cgcgggcgcg tgcgaactcg gcgcgctcct ccggcttcaa
     7441 cgggcgaaat gcgcgcgcgg gccgccccgc ccagacccat tgcgccttca cgacggtgtg
     7501 cggctcgacc cacgcaccgg cgccgatgca tccgccgtgc tcgacgacga cgtgatcgcc
     7561 tacgcgcgag cccatgccga cgagcgcgtc gtcgccgatg atgcccgacc cgagctccac
     7621 gttgtgtccg atggtgacgc cgacacccag cacgagatca ccacgccgcc gatcggtcgc
     7681 gaggacgcag ttgtcctgca cgttgctccg ggcgccgatg acgatgcggc cgtctccggc
     7741 ggacaccgcg cagccgaaga agatgctggc gtcgtcgccc atctcgacat cgccggcgac
     7801 gatggccgtc gcggcgacgt acgcgttcgt cacgcgcggc gagcggccgt gcagcgcacg
     7861 caagggccca tcgttcgcct tcggcagcga ccgcacggcg tcggtccacg cgggcacgtg
     7921 ccgtgaccga agctcggctg cgggacggcc gcttcgcaaa tcgcgcgcga gcgactgcgc
     7981 ttctgccacg gtgatctcgc gcaccggctt cgcaggatgt cccgcataca ggaagccgcc
     8041 cgccagcatc ttgcgcggtg ggacgacgct gtcgccggcg atcacggcat gcgcgccgat
     8101 gcccgcgtcg tccattacga tcgacgactc gccgatcacg acgccgtcgc cgagcgtgca
     8161 ggcgtgcgcg acgccatagc ggccgaccgt cacgtcgtcg ccgatgacgg caccgcgaat
     8221 ccggtcggcg atatgaacgg tcgcgtgatc gccgaaccat gcgccggcgc cgatgcgaac
     8281 gtcctcgccg tctgcgcgca ccgttgcgta gtcaccaagc gtcgatcgcg cgccgatcgt
     8341 cgagcggccg atcagcgcac cgtgcttcgc gacgcgcagc tcgccgccga tggccggcgc
     8401 gtggcgaagg taatcgagga tgaggccgtc gaccatgccg tcagaagacc ggattctccc
     8461 gatacgttcc ccacagcgtc ttcagcttct cgatgatgtc gcccatcgtg gcccccgccg
     8521 aaacgagctc gatcgtgacc ggcatgatgt tctcgtcctg ccgctttgcg acctcgacga
     8581 gacgttccag cagcgcgctc accttcgcat tgtcacgctc gcggcgaacg cgttgcaagc
     8641 gtgcgatctg acgctgcgcg gtcgacgcat cgtacgggtg cagctcgatg gcgagcttct
     8701 cttccggatc gacgaacctg ttgacgccga tgacgggctt ttccccgctg gccttcttca
     8761 atgcggtttc gtaggcgaag tcggcgatgc gccgctggaa ccagccttcc tgcgtgaggc
     8821 gcaccgtgcc gccgcctcga tccacctcgt cgatcacgcc gaagatcgcc cgctcgtact
     8881 gcgtggtcag gctttcgacg aaatacgatc cgcccagtgg atcgacgacg tcggcgacgc
     8941 cgctttcgtg cgcgatgatc tgctgcgtgc gcagcgcgat cttcatcgcc tcctcggtcg
     9001 ggatcgcaaa cgcctcgtcc atgccgttcg tatgcaggct ctgcgcgccg ccgagcaccg
     9061 ccgccagcgc ctgcagcccg gtgcgcacga cattgatcgg gtactgcggc ttggtgagcg
     9121 acgccgccgc cgtctggcaa tggaagcgca gccgcatcga ctcggggttt ttcgcaccga
     9181 agcgatcgtg catgatcttc gcgtagacgc ggcgcacggc gcgaaacttc gcgatctcct
     9241 cgaagaaatc ggcctggcag acgtagaaga acgcaagccg cggcgcgaat tcgtcgacct
     9301 gcatccccgt tctcagcact tcctcgacgt acgcgatcag gttgcacatc gtgaacgcga
     9361 cctcgtcgag cggtgaggcg ccggcctccg acatgtgata gcccgagatg ttgatcgggt
     9421 tgtagcgctt catgttgcgt gcgcagtacg tgatgcagtc gcgcacgatg cgcaccgacg
     9481 gcgcgacggg aaacgcccat tccttctgcg cctggtattc cttcaggatg tcggcctgga
     9541 tcgtccccga cagcgcatgg aggtcgtagc cgcgtttttt tgcgagcgcg acgtacatcg
     9601 cgagcaggat ccacgcgctc ggattgatcg tcatcgacac cgagatcttc gtgaggtcga
     9661 tgccgtcgaa cagatgctcg atgtcggcca gcgtgtcgat cgcaacgcct tcccggccga
     9721 cctcgccttc gctcatcggg tgatcgctgt cgtagcccat cagcgtcggc atgtcgaaat
     9781 cggtcgataa accggtctgg ccgtgcgcga tcaggtactt gaagcgctgg ttcgtgtcct
     9841 cggcggtgcc gaagcccgcg atctggcgca tcgtccacag gcggccgcga tacatcgtcg
     9901 ggtaaggccc gcgcgtgaac ggatagcggc ctggcagacc gatgtcatcg agcggtgtgt
     9961 cggcgacgtc gagggccgtg tacgtacgct tcaaagggaa accgcccacc gtttggaaat
    10021 cgtcggtgcg ctcgggagtg cgcgcaacgt acttcgcgac ttcgttcttt tcccacgcgt
    10081 cgacgtccgc ggcgagcgct gcccgcgcgc gcatgtcggc caaaatcgtc gagtcgttca
    10141 tgcatcacct ccgatgttca gttcgcgcag cagctgccgc gcggcggcgt gcggcgacag
    10201 cgagcgcacg cgcagtcgtt cgagcaatgg ggaaagcttg cccagccgat ggcgggcgaa
    10261 cgcatcgtgc agaagacctt ctccggccgc gagcagccgc cgctcggcga tcgcgcggcg
    10321 gcgcgcctcg atctcgccgg tgcgctcgag cgtttcgcga tggcggtcga tcgcgacaag
    10381 cagctcggcc acgccccgat cctgaatcgc gctggtgccg atgaccgccg gcagcgtgaa
    10441 atcggggtca gagctgtatc tcccggcatg cgcgtcagca gcacgcgaag atccatccgg
    10501 gagatacagc tctgaccccg atttcagctg cgcggtgtcc atatgcgtgt agccgagcgc
    10561 gagcatcgtc ttgaggtcgg cgatcgtccg gttcgcatcg ggccggtcgc atttgctgac
    10621 gacgtggatg tcggcgatct cgagaatccc ggccttgatc gcctggatgt cgtcgccaag
    10681 tccgggcgcg gagacgacga ccgtcgtgtg cgccgcgcgg gcgacgtcga cctcgtcctg
    10741 tccgacaccg acggtttcga tgagcaccat gtcgaagccg gcggcgtcga gcacgtcgac
    10801 ggcttccagc gttcccttcg caagcccgcc gagcgcgccg cgcgtcgcca tgctgcgcac
    10861 gaatacgccg gcgtcgcccg cgagatcgcc catgcgaatg cgatcgccga gaatggcgcc
    10921 gcccgaaaac gggctcgacg gatcgacggc aaccacggcc accttccggc cgcttttgcg
    10981 aatctccggg acgagctttg ccaccagcgt cgacttgccg ctgcccggca cgccggtgat
    11041 gccgactacc tgcgcgcgtc cggcgtgcgc gaacatcgcg tcgagcgtcg gccgcgcctc
    11101 ggcgatgccc gattcggcgc gtgacaggag ccgcgcgatc gcgcgcgtgt ccccggcgag
    11161 gacgcgcgat tcgaggtcag ccgagcggat atacgccacg attcacgagc gaagccgcgc
    11221 gttcagctca cggaacactt cgcgatcgtc gacgacgcgg cgcgccttga agtcggtgcg
    11281 cgggaacgtg ccgggcggca ccacctcgag cctcgagcgc agcccgagca tcttcgacag
    11341 gcgtcgctcc gcgttctcgc gcagcgccga atgcgccgct gcgccctgtg catgctgctc
    11401 gggcgcgatc tcgatccgga tgacgagctc gtccatcgcg ccgtcgcggg tgatcacgat
    11461 ccggtgctcg ccgccgtagc cgtgcagctg gctcagcacc gcatcgacct cgctcggata
    11521 gacgttctcg ccgcggatcg tgaacatgtc gtcgatgcgc ccgaagatgc cctgcggcag
    11581 ccgcggatac gtgcggccgc agggagtggg cccgtgatcc cacagcgtga gatcgccgga
    11641 agcaagccgg atcatcggct gcgacgtgcg ctcgagatgc gtgtatacgg gggtgccgcg
    11701 ttgcccgtac ggcacccggc ggtaggtcgc cggatcgcac acctcggtgt agacgatgtc
    11761 ctgccagcac agcatgccgg cgctttccgc gctgccggcc acgtgcatga acggcgtcat
    11821 ttccgccatc gatccgcaat cgacgacgcg cgcgccgtag gcggcaatga ggcggtcacg
    11881 cacaccgggc accgaggcgc ccggctcgcc ggagaagaac gtcgtcgaaa ggccgaatgt
    11941 gcgcgggtcg tggccttcgt cgcgcgcgac ctcggcgagg tgcagcgcga acgacggcgt
    12001 cgcatacagc gcggcgggct tgaccaggtc gagccactgc gcggcgcgcg ccgtcatgcc
    12061 cggagcgccg gcgccgaacg ggaacgcctt cgcacgcagc cgctcggcac ccgcaagcgc
    12121 tccccacgac cccatgtaca ggctgaagat cgccgcgacg aacacgacgt cgcccggacg
    12181 tatgcccatc ccccacagga tgcgcgcgtg cgcgttcgcg atggcgtccc agtcggcacg
    12241 gccgatggcg aacgcggtcg gccggcccgt ggttcccgac gtcccgtgga tgtgatgcac
    12301 ctccgcgccg ggaacgcaga ggtaatcgcc gaagggcggc acgcgcgcct gtgcgtcgcg
    12361 cagatcctgc ttggtgatga cgggaacctt cgcctcgaag tcctcgagcg agcgcagatg
    12421 gccgggatgg aaccccgcgt cgtcccactt gcggcgatag aacggcgacg tgtcgtacgc
    12481 atagcgcgtg accgccttca gccgctcgag gatcgctctt tcacggtcgc cgggcggcat
    12541 cgtttcgcgc accggaaacc agtagcgcga atcgacgggc ggcacgtagt gcgcgtcgaa
    12601 gcgcggagga aacgaccact gctccatcgg ttccatcgtg gtttgtcgcg tcgccggcgc
    12661 gctcgcccct gatccctgct accgcgggcc gcgctcggcg acgaggcggc ggatcgtctc
    12721 gacgatctgc cgcggcggcg tgtcctgcaa cagcagctcg cgcacgccga tcgccttcag
    12781 cttcggcaga tcctcgtcgg ggatcacgcc gccgccgacc acgaggatgt cctcggcgtc
    12841 gcgctccttc aaaaggccga tgagccgcgg aaatacggtc atgtgcgctc ccgacaggat
    12901 gcttacgccg atcacgtcga cgtcttcctg gatcgcggcc tcgaccacct cgtccggcgt
    12961 gcggtgcagg ccggtgtaga tcacatccat gccggcgtcg cgcaacgtcc gcgcgacgat
    13021 cttcacgccg cggtcgtgac cgtcgaggcc gactttcgcg atcaccacgc gaatcggttg
    13081 cgttgattgt tccacccgcg atcctcctcg gattccctcg aaaagcgaac gctgcgaggc
    13141 ccgctcgata gtattccgaa tacagtatgc aaaccgccaa tgcctgtcaa cctgaatctg
    13201 cgctagcgtg tcggcgcatc caatcccgag tcccgaaagc ccttcgatgc cccgcacgtc
    13261 gctcccctgg ctggtcctcg caaccatcgt cgtctcggcg gcgctcgcct ggtggacgct
    13321 cacgcgtacc gtcggcggcg agcccgccgc gcgtttggaa catcgcgacc tcgcgccgtt
    13381 tcacgagatc gaggtcggcg gcagcgccgc ggtgacgctg ctgcaggccg aggccgaatc
    13441 gatcgacgtc gaagccgcga gtcgcaccag cgtcgctgcg gaagtcgcca acggccggct
    13501 cgtcgtccat gcgcgcgacc ggcgccgctg gtggaacggt ctgctcggcc ggcgctcatc
    13561 cgcgacgccg cacgtgacgg tacgctttcg cacgctcgac gccattgcgc tgacggggaa
    13621 cgtgaagatg gatgcgccga agctttcgac gacggcgtta cgcatcgatg cctcgggcgg
    13681 atcggcgctt tcgatcgacg atcttcgcgc cacgtcgctg cgggtgaacg gctcgggcgc
    13741 gctcgatgca aagctcgccg ggcgcgtcga gagggaggac gtgtcgatct cgggcgcggg
    13801 gtcgtaccgt gccgagcacc tcgtcgccac cgacgccacc gtttccgtca gcggcgtcgg
    13861 caacgtgatc gttcacgcca cgagcacgct cggcgcaagc atttccggcg ccggcgtcat
    13921 cgaatacgcc ggcaatcccg cggtgaccga gcacgtgagc ggcatcgggc gcgtcaagcg
    13981 gcgcgcgccc gagcagcgat cgggactcac gaccgtggcc atccacgctc aatgcagcgg
    14041 cgtctccgcc gacgcaccgt ggtccttgaa gaacagcggg ccgccggtga ccggatcgat
    14101 gtcggcgtgg acacccggca tgcagcgggg cgccgacacg cggcagtcgc gcaggagcgc
    14161 aatgagcatc gcggcgacgt cctgcacggt ttcgtaggga taaagcggac agcgaaagcc
    14221 ctccgccgcc ttggcggcga acggcgagga taacgacagg cgcaactccc cgccgcccgg
    14281 ggcgtccgcc gcgtgatgcg cgctcacgat tgccgtcggc tcgccgtacg acgcacggat
    14341 cctgcgaatg gcgttgctcg cgaagagctg tgccgatacc tcgcgttgtg cggcccgacc
    14401 cgattgcacg gcggccacca gacgctgcgc gggacgcggc agcgcgagcc gcgtgacgcc
    14461 cggtgcgccg agcgccttcg cgatcgctgc cgcgagcgcc atcccccacc ggccgatcgt
    14521 cgattcggcg agcggatcga ttcccggcgg cgtcagcagg gcgccggcaa tgaaccgcag
    14581 gtgcacgcgc tcgacgcccg catcgaccgg aatcggcgcg ggaggaagat cgagtgcgcc
    14641 gcccgcgaaa ttgccgcccg catgcgcgac gagcgcgcat cgcgcgagaa gcgcgggcaa
    14701 cgccgcgacg tcgacccggt cggcgctgac cagcgacgac gacaacgcaa acgtctcgca
    14761 tccgccgaag gcgcgcgcat cgcgcaggat cgcggcgagc gcggcgacgt cgggaagcac
    14821 cgcgccgagc gtgaccggcg cagcaccggt gtcgagtccg gcgacgacga tcaccgggat
    14881 cgcgaagagc gtcgtgtgca cctcgccgga cccatggagc ggcgcgcgct cgacgtcgtc
    14941 gagcaggcgg cgcagatgtc ttgcgatcgc gggcgacggt gcctctccaa gcgtgcgggc
    15001 gagaaggtcg ccgtccccgg ccgcaagcgc ccgcgcgacc caatcgcgca gctgcgcgtc
    15061 gagcgcatcg gcgtcggcac gtgtcgccgc gtcgatggcg cgctgcgcgt cggcgtaggg
    15121 cggcatcgcc gcattcgccc ggggccaggc gcgcgggtcg tcgaggtcga gggcgtacat
    15181 cgcggttcgt cgattcacgc cgacaggcgc gccggtgatt ctcgcacgca tgccgcgtcg
    15241 ccgatggaaa gggcgataaa atcgcggtcc ctgtccgccc ccggtgaaaa gcgctcgatg
    15301 cgcgtctcga agttttatct cagcacgctg aaggaagcgc ccgccgaagc ggaggtcgcg
    15361 agccagaagc tcatgctgcg cgccgggctc atcaagaagg tgtcgtcggg catctacagc
    15421 tggatgccgc tcggcctcat cacgctgcgc aaggtcgaac aggtggtgcg cgaggaaatg
    15481 aaccgtgcgg gcgcgctcga aacgtacctg ccgcacatcc tgcccgccga gctatgggag
    15541 gagacgggcc gctgggacaa attcgggccg ctgctcctga agatcaagga ccggcaggcg
    15601 cgcgatttcc tgttcggacc cacgcacgag gaggtgatga ccgacatcgt gcgcaaggac
    15661 atcaggagct accgccagct cccggtcaac ctgtaccaga tccaggtcaa gttccgcgac
    15721 gagatcaggc cgcgcttcgg cgtgatgcgc gggcgcgagt tcctgatgaa ggacgcgtat
    15781 tcgttcgacg ccgatcgcga cgcgttgatg gtgtcgtacc gcgggatgta cgacgcgtat
    15841 gcgcgcatct tcacgcgcct cggcctcgag tttcgtgccg tcgaggccga caccggcgcg
    15901 atcggcggct tcgcctcgca cgagttccag gtgctggccg attccggcga ggacgccatc
    15961 gcctacagca atggctcgac ctacgcggcc aacgtcgagc aggcggaagc catcgcgccg
    16021 tcgacgccgc gtgcggcgcc gtccgaagcg atgcaacgcg tgcccacgcc gggaagctcg
    16081 acgtgcgagc aagtggcgga gctgctcggg cttccgctcg cgcgcacggt caagtgcctg
    16141 ttgatccacg cgcaggatcg cgtgcagatg ctgctcgtgc gcggcgatca catgggcaac
    16201 gaagtgaaga tcggcaagct gcccggcctc gacgggtggc gcttcgcgag cgaagcggaa
    16261 gtcgtcgccg ccaccggctg ccggcccggt tacctcggcc cggtcggcat cgcgggcgac
    16321 atgccgctca tcgtcgaccg gcaggtggcg gcgatgagcg acttcgtctg cggcgcgaac
    16381 gaggccggtt accatctgcg cggcgtcaac ttcggccgcg actgccgcga gcccgacgcc
    16441 gtcgccgata tacgcaacgt cgtcgagggc gacccgtcgc ccgacggcag gggcacgctg
    16501 tcgatcgtgc gcggcatcga ggtcggccac gtgtttgcac tgggcgacgt gtactccaca
    16561 gccatgggtg cgagttacct cgacgccgac ggccgctcgc aggtgctgca gatgggctgc
    16621 tacggcatcg gagtgacgcg cgtcgtcgcc gcggcgatcg agcagaacca cgacgaacgg
    16681 ggtatcgtct ggccttcggc gatggcgccg ttcgcggtgg cgattgcgcc gatcggctac
    16741 gacaggagcg acgcggtgcg cgagtgcgcc gaccggctgc accggcaact cgccgaagcc
    16801 ggcgtcgagg tgctgctcga cgatcgtggc gagcggcctg gcgtgatgtt tgctgatctc
    16861 gagctgatcg gcatcccgca tcgggtgacg gtcggcgagc gcggattgaa ggaaggtatc
    16921 gtcgaatacc aggctcgccg cgacgcgacg gtcacgaggc tgccgctcgc ggacacgctt
    16981 gcttttctca acgaacggat cggaacctga tgcagccgta ccgggtatcg cgtccgcgaa
    17041 cgatgttcgc ggcgctcgcg tttcttgcgg cctgcgcagc gccgctcgac gcacacgccg
    17101 gcgcgcaggt cgaggagcgt ctcgctccgt cggtcgcaag cgggctttcg cgtgccgtcg
    17161 ccgacaaccc ggtgccgcgc aactacatcg agcgcgcggc ggttcgcgag tggctcgcgg
    17221 cgatgtcgcc gaagctcgcg ggccgtttcc ccgatgaagc cgcacgcgtc gagtttctcg
    17281 ccaccgtgca ttacgaagcg acgcgcgccg gcctcgatcc gcagctcgtg ctcggcgtca
    17341 tccatcacga gagcaatttc cgcaagtacg cggtctcgtc ggccgacgcg cgcggcttca
    17401 tgcaggtcat gccgttctgg gtgcgcctga tcggcacggc cgaccagaac ctgttccagc
    17461 tgcgcacgaa cctgcgttat ggctgcacga tcctgcggca ctacctcgac gtcgagaacg
    17521 gcgacgtcta tcgcgcgctc ggccgctaca acggcagcct cggccagccg gagtatccga
    17581 cggcagtgat gaccgcgatg aagcgctacg agccggggac gtcggtggcc gccgtgcgca
    17641 cgtcggcgac cgcttcgctg cactgagcgc ctcgggtccg ccggaaggtc cgtaccgcgg
    17701 ccgtttcgcg ccgtcgccca ccggtccgtt gcacttcggc tcgctcgttg ccgcgatggc
    17761 gagctatgcc gatgcgcgcc gcaacgccgg cgcatggctc gtgcgcatcg aggacgtcga
    17821 cgttccgcgt gcacgtccgc gcgccgcgga cgacatcctg cacgcgctcg aaggttacgg
    17881 cttcgagtgg gagggacccg tgtggcgcca atcgtcgcgc cgcgacgcgt acgaggcggc
    17941 gcttgcccgg ctcgccgcgg ccggcctcgc ctacgcctgc gtgtgcacgc ggcaggcgct
    18001 tgccgcgcag ccgatcggcc gaattggcga acgcgtgtat ccgggaacgt gccgcgatgg
    18061 catcggccat cacggtgacc gggccgcgga gcgcacgcga cgagccgccg tgcgcgtgcg
    18121 cgtgccttcg gcgcggatcg cattcaccga tcgactccac ggactgcagc agcagcggct
    18181 cgacgaggac gtgggcgatt tcgtcgtccg gcgcagcgac ggactctgcg cgtatcaact
    18241 cgccgtcgtc gtcgacgacg ctgcgcagcg catcaccgac gtcgttcgcg gcgcggatct
    18301 tctcgcgtcg acaccacgcc agatcttcct gcaacgcgcg ctcggcgttg cgacgccacg
    18361 ctatctgcac gtgccggtcg ccgtcgacgc acgcggtgcg aagctgtcga agcagaccgc
    18421 ggcaacgccg ttgcccgctg caccgctgcc ggcgttgcac gccgcatggc gctttctcgg
    18481 acaatgcgca ccgtccgatc ccgcgtgccc ggtgtccgag ttctggacat gggcgatcgc
    18541 ccactgggat gccgggcgca ttcccgcggc cgccacccgg cccgccccga ccgcgatcgt
    18601 ataatcgggc cacgcgcggc gacggattcc cggtcgccgg cgcattcatt cccgtcctca
    18661 tcctgccgat gaccactctc gtcgtcgtcc gcaagggcga ctccgttgcg atcgccgccg
    18721 attcgctgac gacgttcggc gatacgcgcc tcgccgccga gcacgatcgc acctatgaca
    18781 agatcgtccg ctatcgcgac acgtacatcg ggctctgcgg gtccgctgcc caccagctcg
    18841 tgttcgaaag cctcctcgcg aagcacggcg atctcgactt cgcgcacaaa tctgcgatct
    18901 tcgagacgtt tcgcaagctc cacccgatcc tcaaggagca gcacttcctg aatccgaagg
    18961 aggaagagga cgatccgtac gaatcgacgc agatcaccgc gctgatcgcc aacgccaacg
    19021 gcatctttgg cgtcttctcg atgcgcgagg tgttcgagta cacgcagttc tgggcggcgg
    19081 gatcggggcg cgaattcgcg ctcggcgcaa tgcacgcgcg ctacacgcaa acgaagacgg
    19141 ccgaaacggt ggcgcggatc ggcatcgagg ccggcgccaa gttcgaccgc aattccgcgc
    19201 tgccgatgac gctctacacg ttgccgctga cgaagcaatg agtccgcccc gcgtcgcgga
    19261 cgcgctcgtg ccgttcgtgg tcgagcacgc gccggtgcgc ggatcgctga tccgcctcga
    19321 ggccacgagc cgcgacatcg tcgcgcagca cgactacccg ccgccgctcg cccgcgtgct
    19381 gtccgagctt tgcgcggccg ccgcgctgct tgcctcgacg ctgaagctcg atggaagcct
    19441 cgtcgtgcag cttgcaggcg atggcccggt gcgtctgctc gtcgtcgaat gcaccgatgc
    19501 gctcgaactg cgggccacgg cgcagtggga ccggcgccgc gtggcagcac tcggagaaaa
    19561 tgcgtcgctc gcggcactgt ccggcggcgc cggacacgga cggctcacgc tcacgctcga
    19621 tcagcgcggc gccggaacgc tgtatcaggg cgtcgtgcgt ctcgacgccg cgtccgtcgc
    19681 ggcatcgatc gagcactatc ttgccgcatc cgagcagctg caaagccggc tgtggctctt
    19741 gccccgggac gcaggcgtca ccggcctgct gctgcagcga ctgcccgacg cgagcgctgg
    19801 cgatttcacc ggatggccgc gtcttgtcga cgatgccggt cgggcgatcg cgcgcgccgt
    19861 acgcggcgcc acgttggaag cgattctcgc cggtctgttc ccccgtaacg acattcgaat
    19921 gttcgagccg cggccggcgc gcttccgctg ccgctgctcg acggaccggg tggcccatgc
    19981 gctgcgcatc gcgggccacg ccgagatcga ggcggcactt gccgcgcgtg gcatggtcga
    20041 ggtgacgtgc gagttctgca accgccgcta cacgttcggc ccggacgagg cgcgcgcgct
    20101 gtttgcacag gcaccggacc caggagcgac ttcgacatga gcgtgtacac gccgtccacc
    20161 ttcgcaatca ccgaccgtgc cgcgatcgcg cgactgatgc acgaccatcc gttcgcgacc
    20221 ctcgtcacgc ccgcgacgcc cgagccgttc atctcgcatc tgccgttgct gttcgttccc
    20281 ggctgcgagc cgcacggcac gctgatcggg cacatggcgc gcgcgaaccc gcattgggag
    20341 cacgcggcga atgtcgagtc gatcgcgatc ttccacggtc cgcacgccta tgtatcgccg
    20401 tcgtggtacg ccgagccggc gaaagcggtc ccgacctgga actacgcggc cgcccacgtt
    20461 cacggcacgc tggaagtcat tcgcgatccg ctggagacgc gaagggtgct cgacgcgctc
    20521 gtgcagcg
//
LOCUS       DASSVX010000050         5463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_35037, whole genome shotgun sequence.
ACCESSION   DASSVX010000050 DASSVX010000000
VERSION     DASSVX010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35037"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..898
                     /gene="imuA"
                     /locus_tag="VFO53_02380"
     CDS             149..898
                     /gene="imuA"
                     /locus_tag="VFO53_02380"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HET8875151.1"
                     /translation="MTSTTHSAAIARLLAHPAIWRGGDCASESASVASGFAELDAALP
                     GGGWPRGALTELLVEREGIGEVRLTLPALVRLQAEGRSVVWIAPPHRPYAPALAAAGL
                     DLARLIVIRGCSSAEALWAYEQALRAPECGAAFGWMQAHEDRALRRLQVAAREGRTWG
                     VLWRRPGQRASATAAPLRLALSPQQTRLAVRVLKRRGAELAHPVVLDLARTPGAPLYP
                     LPDGMPILQRTSTSPPPARQHRLRTSQRKAS"
     gene            973..2499
                     /locus_tag="VFO53_02385"
     CDS             973..2499
                     /locus_tag="VFO53_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HET8875152.1"
                     /translation="MLWTCVLLPSLALDVFARAAPDDTERPFVVASGGHYPRVVAANG
                     CANAAGIRGDQLISAALAFAPDVVVRDRDGDAEAAALAEVATLLLAFTPQVSIVHSNA
                     VVAEIAGSMRLFGGLTQLLALLGASVESRGYARRMAVAPTPTAALMLARAGHTQPVLR
                     RSALPDVLSPLPLTLLDFEPTTLATLKAAGVTTFGAAHALPRAGLARRFGQHVVDRLD
                     RALDRAPDPQRSYEPPPRFERKLVLPAPIDNVEALGFGVNRLIGDLAGWLLARGLGVV
                     RMSLALIHERYVRDRGIPPSAAEFRLGAPARAPAHLHAVMRERLARVTLPASVEAIVL
                     TSDETAPLAGRNFGLLPEDEVDAVVVPLVDRLRSRLGDDVVTVLATHPEHRPEYATSA
                     TTPETRSSQPRTHAHSTGPQKRNRKASPLEQPSAATDAPRPLWLLDEAKILSGALETA
                     PWILRDGPERIESGWWDGHDLRRDYFVAETPQGGMVWIYRDHRYGTDDGEWFMHGVFA
                     "
     gene            complement(2506..3282)
                     /locus_tag="VFO53_02390"
     CDS             complement(2506..3282)
                     /locus_tag="VFO53_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HET8875153.1"
                     /translation="MIDRDGGARRRFLRGVAASFGALGVAGCDRLSQSEWFPKVLESA
                     ERLNERVAKVVTGRKAMAQEFSAADLSPTFRSNGTAEPNNPEYAALAAKGFDGYALDV
                     SGLVAQPRRFSLAELRALPSRTQITRHDCVEGWSAIGKWQGARLSAVLDAVQPTAAAR
                     YVVFHCADPMDDDGGSPYYESIDMDDAYHEQTLLAYALNDKPLPIANGAPIRLRVERQ
                     LGYKHAKYVMRLELVDSFAKIGEGKGGYWEDQGYQWYAGI"
     gene            complement(3316..4182)
                     /locus_tag="VFO53_02395"
     CDS             complement(3316..4182)
                     /locus_tag="VFO53_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003551227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET8875154.1"
                     /translation="MNDQAPPVAAPLAFDTERSRPLYLRHALAVRIMHWINVVALTLM
                     FMTGLQIFNAHPALYWGASSYSGRPAWLEIGAREDPQGKPVGYTRLLGREFTTTGVLG
                     LSVDGGRPYAQGFPAWLTVPSHQWLSMARTWHFFFAWVFVINGLCYVIYSVAARHLKR
                     DLLPTRADIRGIGASIVDHLRFRHPTGEAAKRYNVLQKFAYLTVIFVLLPLIVLMGLG
                     MSPRLDTLFGGWVNWFGGRQSVRSIHFIIAWMLVAFVLIHVFEVIVTGFWNNLRSMIT
                     GRYRVPKEAGHG"
     gene            complement(4288..>5463)
                     /locus_tag="VFO53_02400"
     CDS             complement(4288..>5463)
                     /locus_tag="VFO53_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8875155.1"
                     /translation="PPGVWTLGFVSLLMDASSELVHALLPLYMVGALGASMLVVGIVE
                     GVAEAIALFVRLFSGYWSDALARRKPLVVLGYGLGALSKLAFPLATALSVVIAARFVD
                     RIGKGIRGAPRDALIADLTPPSVRGAAFGLRQALDSAGAVLGPLLALGAMAYFAGDFR
                     HAFWVAVPPAAMCVALLWLGVRDADARAASASDTRRRLSWRDARRLDRRFVIVTAIAS
                     VLTLARFSEAFLVLRGQDVGLGTQGAPWVMVAMTIVYAGCAWPAGKLADRGHAKHLLA
                     AGLAALVASDLTLAFAAKIPGVLAGACLWGLHMALTQGLLSALIAATAPAELRGTAFG
                     VFAVASGIALLVASALAGYLWQSIGPSATFFAGAAFAVIAGAGLATQLRDIPKLGGR"
BASE COUNT          969 a         1889 c         1749 g          856 t
ORIGIN      
        1 cagcaccttg cacgtcatcg accggcgttt cacgacacgc tgcaacgagt cgaatcgcat
       61 cctgaagtcg cgactatctt tgcgcgtcat tggccgccaa gctcgacgcc ctcgacaccg
      121 aacgcggcga catgagcgcg attcgtccgt gacatccacc acgcattccg cggcgatcgc
      181 gcgcctgctc gcgcatccag ccatctggcg cggtggtgat tgcgcgtccg agtctgccag
      241 cgtcgcgtcc ggttttgccg agctcgacgc agcgcttccc ggcggtggat ggccccgtgg
      301 cgcattgacg gagttgctcg tcgaacgcga aggcatcggc gaggtgcgac tcacgctgcc
      361 agcactcgtc aggctgcaag cggaagggcg cagcgtcgtc tggatcgcgc cgccgcaccg
      421 gccgtatgcg cccgcgctcg ccgcagcggg gctcgacctt gcacgcctca tcgtcattcg
      481 cggctgcagc agtgccgaag cgctgtgggc ttacgagcag gcgctgcgcg cgccggagtg
      541 cggcgctgcg ttcggctgga tgcaggcgca cgaggatcgc gccttgcggc gactgcaggt
      601 tgccgcgcgc gagggccgca cgtggggcgt gctgtggcgc agacccggcc agcgcgcaag
      661 tgctacggcc gcaccgttgc ggctggcgtt gtcgccgcag caaacgagac tcgccgtgcg
      721 cgtactgaaa cggcgcggcg ccgaactcgc gcatccggtc gtgctcgatc ttgcgcgcac
      781 gcccggtgcg ccgctttatc cgttgcccga cggcatgccg atattgcagc gaacgtcgac
      841 atcaccaccg ccagcccgtc agcatcggtt gcggacttcg cagcgcaagg catcgtgatc
      901 tctgtagcgc catgcacacc catgcattgc agcgtcgcaa aacgccgggc gtaaacgcgc
      961 tgatgcccat cgatgctgtg gacctgcgta ctgttgccat cgcttgcgct cgatgtcttc
     1021 gcgcgtgcag cgcctgacga tacggagcgg ccgttcgtcg tcgcaagcgg cggccattac
     1081 ccgcgggtcg tcgcggcgaa tggttgcgcg aatgcggcgg gcattcgcgg cgaccaactg
     1141 atctccgccg cgctcgcctt cgcacccgac gtcgtggtgc gcgaccgcga tggcgatgcc
     1201 gaggcggctg cgctcgccga agtcgcgacg ctgctgctcg cgttcacgcc tcaggtgagc
     1261 atcgtccatt ccaacgcagt cgtcgcggaa atcgccggca gcatgcggct cttcggggga
     1321 ctcacgcaat tgctcgccct gctcggcgcc agcgtcgagt ctcgcggtta tgcgaggcga
     1381 atggccgtcg cacccacccc gacggcggcg cttatgctcg cgcgcgcggg tcatacgcag
     1441 ccggtgctgc gccgctctgc attgcccgac gtgctctcgc cgctgccgct cacgctgctg
     1501 gatttcgagc cgacgacgct cgccacgctg aaagcggcag gcgtgacgac atttggcgct
     1561 gcgcacgcat tgccgcgtgc cgggcttgcg cgacgcttcg gccagcatgt cgtcgatagg
     1621 ctcgatcggg cgctcgatcg agcgcccgat ccgcaacggt cgtacgagcc gccaccgcgg
     1681 ttcgagcgca agctcgtgct gccggcgccg atcgataacg tcgaagcact gggcttcggc
     1741 gtcaaccgcc tcattggcga tcttgcgggc tggctgctgg cgcgcgggct cggcgtcgtg
     1801 cgcatgtcgc ttgcgctcat tcacgagcgc tatgtgcgcg accgcggcat accgccctcg
     1861 gccgcggaat tcaggctggg cgcgccggcg cgcgcaccgg cgcatctgca tgcggtaatg
     1921 cgcgagcgtc ttgcgcgcgt gacgctgccg gcatccgtcg aagcgatcgt gctcacgagc
     1981 gatgagacgg caccgcttgc ggggcgcaac ttcggcctcc tgcccgagga tgaggtcgac
     2041 gccgtcgtcg tgccgctcgt cgatcgattg cgctcgcggc ttggcgacga tgtcgtcacc
     2101 gtgctcgcca cgcatcccga gcatcggccc gaatatgcaa catccgccac tacgcccgaa
     2161 acgcgcagca gtcagccgcg cacgcatgcc cattccacgg ggccgcaaaa acgcaaccgc
     2221 aaagcgagtc cactcgaaca gccgagcgct gccaccgacg cgccgcggcc actgtggctg
     2281 ctcgacgagg caaagatatt gagcggtgcg ctcgaaaccg cgccgtggat tttgcgcgac
     2341 ggcccggaac ggatcgaatc aggatggtgg gatggccacg acctgcgccg cgattacttc
     2401 gtcgccgaaa cgccgcaagg cggcatggtg tggatttatc gcgatcatcg ctacggcacc
     2461 gatgatggcg agtggttcat gcacggcgtg ttcgcgtaac ccgcctcaga taccggcgta
     2521 ccactggtag ccctggtcct cccagtagcc gcccttccct tcgccgatct tcgcaaagct
     2581 gtcgacaagt tcgagccgca tcacgtattt cgcgtgcttg tagccgagct ggcgctcgac
     2641 gcgcagcctg atcggtgcgc cattggcgat cggcaacggc ttgtcgttca acgcgtacgc
     2701 gagcagcgtc tgctcgtgat aggcatcgtc catgtcgatg ctctcgtaat acggcgagcc
     2761 gccatcgtcg tccatcggat cggcgcagtg gaacacgaca taccgcgcag cggccgtcgg
     2821 ctgcacggcg tcgagcacgg ccgaaagccg cgcgccctgc catttgccga tcgcgctcca
     2881 accctcgacg caatcgtgcc gcgtgatctg cgtgcggctt ggcaacgcgc gaagctcggc
     2941 aagcgagaac cgccgcggtt gcgcgacgag cccgctcacg tcgagcgcgt agccgtcgaa
     3001 gcctttcgca gcgagtgccg cgtattccgg attgttcggc tccgcggtgc cattgctgcg
     3061 aaacgtcggt gacagatccg cggcgctgaa ttcctgcgcc atcgccttgc ggccggtgac
     3121 gaccttcgcg acgcgctcgt tgagccgttc ggccgattcg agcactttcg gaaaccattc
     3181 gctttgcgac agccgatcgc agccggcgac gccaagcgca ccaaaggatg ctgcgacgcc
     3241 acgcaagaaa cggcgccgtg cgcctccatc acgatcgatc atccgagtct ccgcccatcg
     3301 actgtcgtca agcgatcacc catggcctgc ctccttgggc acgcgatagc ggccggtaat
     3361 catcgagcgg aggttgttcc agaagccagt gacgatcacc tcgaacacgt gaatcagcac
     3421 gaacgccacg agcatccacg cgatgatgaa atggatcgat cgcaccgatt ggcgaccgcc
     3481 gaaccagttg acccatccgc caaacagcgt atcgagtcgc ggcgacatgc ccaagcccat
     3541 cagcacgatc aacggcagca gcacgaagat gaccgtcagg tacgcgaact tctgcagcac
     3601 gttgtagcgc tttgccgctt caccggtcgg atgtcgaaag cgcaggtgat cgacgatcga
     3661 ggcgccgatg ccgcgaatgt ctgcgcgagt cggcaggaga tctcgcttga gatgtcgcgc
     3721 agcaaccgag tagatgacgt agcacaggcc gttgatgaca aacacccatg cgaagaagaa
     3781 atgccacgtc cgcgccatcg acagccattg atggctcggt accgtcagcc acgcgggaaa
     3841 tccctgcgcg tacgggcgcc cgccatcgac tgacaatccg agcaccccgg tcgtcgtgaa
     3901 ttcacggccg agtaaacgcg tgtaaccgac cggtttccct tgcggatcct cgcgtgcgcc
     3961 gatctcgagc cacgcgggtc gccctgagta cgacgacgca ccccagtaca gcgcgggatg
     4021 cgcgttgaag atctgcaggc cggtcatgaa catcagcgtc agcgcgacga cgttgatcca
     4081 gtgcatgatc cgcacggcga gggcatggcg cagatagagc gggcgcgatc gctcggtgtc
     4141 gaaggcaagc ggcgctgcaa cgggaggggc ttgatcgttc atgcggttcc tcgcggacgc
     4201 gcggcgcgct ggcacttcgc tggtgcgcaa cgccgctcta ctctagtcgg ccaacacggc
     4261 gaaagcttac gcccgccagc gcatcgatca acggccgcca agcttgggga tgtcccgcaa
     4321 ctgcgtcgcg agccccgcgc ccgcgatgac ggcgaacgct gcgcctgcga agaaagtcgc
     4381 cgatggtccg atcgactgcc acaggtaccc ggcaagcgca ctcgcgacga gcagtgcgat
     4441 gccgctcgcg actgcgaaaa cgccaaacgc cgtgccgcgc agttctgccg gggccgttgc
     4501 cgcgataaga gctgagagca gcccttgcgt gagcgccatg tgcaaccccc acaggcacgc
     4561 gcccgccagc acacccggta ttttggcggc aaacgccagc gtgaggtcgc ttgccacgag
     4621 ggcggcgagg cccgccgcca gcaggtgctt cgcgtgaccg cggtcggcca gcttacccgc
     4681 gggccacgcg caaccggcat agacgatcgt catcgcgacc atcacccacg gtgcgccctg
     4741 cgtgccgagg ccgacatcct gcccgcgcag cacgaggaat gcctccgaga accgcgcgag
     4801 cgtcagcacc gacgcgatcg cggtgacgat gacgaatcgc cgatcgaggc gacgcgcgtc
     4861 gcgccacgac agccttcgtc gcgtatcgga tgcgctcgcc gcgcgcgcat cggcgtcacg
     4921 cacaccgagc cacagcagcg cgacacacat cgccgctggt ggaacggcaa cccagaacgc
     4981 atggcggaaa tcgccggcga aataggccat cgcgcccagg gccagcagcg gccccagcac
     5041 cgcacccgcg ctgtcgagcg cctggcgcag tccgaacgcc gcaccgcgaa ccgacggcgg
     5101 cgtcaaatcc gcgatcagcg cgtcgcgcgg cgcgccgcgt atgcctttgc cgatccgatc
     5161 gacgaagcgc gcggcgatga cgactgaaag cgctgtcgcc agcggaaacg cgagcttcga
     5221 caatgcaccg agcccgtaac ccagtacgac gagcggcttg cgtcgcgcga gcgcatcgga
     5281 ccagtagccc gaaaacaaac gaacgaagag cgcaatcgcc tcggcgacgc cttcaacgat
     5341 accgacgaca agcatcgacg cgccaagcgc accgaccatg taaagcggca acagcgcatg
     5401 gacgagctcg ctcgacgcat ccatcagcag gctgacgaag ccgagcgtcc acacgcccgg
     5461 cgg
//
LOCUS       DASSVX010000051         7622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_768277, whole genome shotgun sequence.
ACCESSION   DASSVX010000051 DASSVX010000000
VERSION     DASSVX010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768277"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFO53_02405"
     CDS             complement(<1..292)
                     /locus_tag="VFO53_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HET8875156.1"
                     /translation="MIRDEHRETWREALFNRRMLICVFTGFSSGLPLYLLISLLPAWL
                     RTGGVDLKTIGFFALIQIPYTWKFLWSPLLDRYALPLLGRRRGWMLVTQLVLL"
     gene            complement(289..921)
                     /gene="metW"
                     /locus_tag="VFO53_02410"
     CDS             complement(289..921)
                     /gene="metW"
                     /locus_tag="VFO53_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HET8875157.1"
                     /translation="MTGVRQEDTALTPISVNGVARADYATIGGWVGQQAHVLDLGCGD
                     GELLAFLAAQRAASGYGIEIDDAGVLACVRNGINVIQSDLERGLAGFDDGAFDVVILS
                     QTLQAMRRIEAIVGEMLRVGREAIVTFPNFGHWTHRLQILRGRMPVSSSLPYQWYDTP
                     NIHLCTVADFDAFLAERGYRVLDRVILAKGRAVGFAPNLTGELAIYRFRR"
     gene            complement(918..2144)
                     /locus_tag="VFO53_02415"
     CDS             complement(918..2144)
                     /locus_tag="VFO53_02415"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HET8875158.1"
                     /translation="MPSFTDTTASADAPAAAARSVGPVLPQVAHFDAPLPLACGRTLP
                     QYDLAYETYGTLDASRSNAVLICHALNASHHVAGFYADDPDNIGWWDNMVGPGKPLDT
                     GRYFVVGMNNLGSCFGSTGPVSSNPATGEPWGSDFPIVTVEDWVDAQAQLADRLGIRQ
                     WAAVMGGSLGGMQALDWAIRYPSRIRNALVIAAAPNLSAENIAFNEIARQAIMTDPEY
                     HDGNFGAHGVKPRRGLRVARMIGHITYLSDEQMEAKFGRQLRDGLNFSFAPEFQIESY
                     LRYQGEKFAEYYDANTYLRVTKALDYFDPATREGGDLARALGPATCRFLVVSFTTDWR
                     FPASRSREIVKALVRKRRDVSYAEIAAPHGHDAFLLDNAQYHAVVRAWFERMASEIGD
                     IGEAGVRARSPNAFSA"
     gene            complement(2229..3215)
                     /locus_tag="VFO53_02420"
     CDS             complement(2229..3215)
                     /locus_tag="VFO53_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET8875159.1"
                     /translation="MDRARAVEALLTHRDAPPAPFRYRFTAMASVHELHLSTPDRALA
                     DRVAAAAIADVRRIESKYSRYRDDSVTSAINRAAGRAAVAIDAETAALLRYADRCHAQ
                     SGGRFDITSGVLRRAWDFKRRPPRVPSHATLAAARELIGWDRVEWSDAAVRLPHAGME
                     LDFGGIGKEYAADRAAVICRDLGIVHALVNLGGDVRAIGPRADGSPWCVGIRDPRRED
                     ATIASILVDDAAVATSGDYERYFEVGGRRYCHLLDPRTGLPVSQWQSVSVAAPLAILA
                     GSYATIAMLLERDAEPFLAPLGVAFLMVAQDGSVTVRDASCAAPAFVVSCAR"
     gene            complement(3182..4354)
                     /locus_tag="VFO53_02425"
     CDS             complement(3182..4354)
                     /locus_tag="VFO53_02425"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HET8875160.1"
                     /translation="MAATEEFPLSAARRRANADTSTDRSAALIAAALALPGMLPATSL
                     AQSAPDEGVIALRYYDYRDWQPGADRISVRSPSLYALVPLSDTLTVEGSLVYDSVSGA
                     SPLYHDTVSGASGRGIGDYRSAADAKLTRYFGSSALALGAAYSREQDYISRAASVEWR
                     TWSADRNRTYAVGVGAAKDRINAPEGVNGPVLDRPRETYDYLLGITQVLDARSLVESS
                     LTWSGGRGYYADPYKLHDTRPNRRQILVWLTHYNRYFPAPDATLRLAYRYLDDSFGDR
                     SHMLEASWVQALPHGLTLTPTLRYLTQSAASFYRDPPFPEGFVPGQLYTADARLSAFG
                     AVTLGLRLAKELPHGVTADLAIDVYRQRGDWRIGGGSANLAEFSARWIELGLSKRF"
     gene            complement(4341..4502)
                     /locus_tag="VFO53_02430"
     CDS             complement(4341..4502)
                     /locus_tag="VFO53_02430"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HET8875161.1"
                     /translation="MTPPKPWEKGDLARSSMRFDSDPLEAKLTQHIYQSKEGAGGGGS
                     VGGGGCGCN"
     gene            complement(4552..5043)
                     /locus_tag="VFO53_02435"
     CDS             complement(4552..5043)
                     /locus_tag="VFO53_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET8875162.1"
                     /translation="MRLLLAFLLAGLTCAASTHAANPGTPAPPFALSTASGETVALAA
                     LRGRVVYVDFWASWCTPCRRSFPWMNAMQARYAGEGLTVVGVNVDKRRVDAQRFLRDT
                     PASFTVAYDAEGTTPSAYDVRGMPSSYLIDRQGNIAAVEEGFHDERRAALEARIRALL
                     AQH"
     gene            complement(5099..6832)
                     /gene="ptsP"
                     /locus_tag="VFO53_02440"
     CDS             complement(5099..6832)
                     /gene="ptsP"
                     /locus_tag="VFO53_02440"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET8875163.1"
                     /translation="MSFALHGIGVTGGIAIGRAQLVSHATREVSHYTIRDQQVPSEIA
                     RFENAVKDVQRELEGLHAALTSGDVPGEFGAFLDVHWMILNDPTLSEAPKRIIAEQSC
                     NAEWALARQMGVLVEQFDQIEDAYLRERKADVVQVGERVLKRLMGRPGTLPAPSAEEQ
                     TILVAHDLSPADVIQFKHHHFAAFLTDLGGVTSHTAIVARSLNVPAVVATHNARQLIR
                     DGEMLIVDGTHHVVIVNPDRAVLAEYRLKQDEFDLARQKLKRLRSKRAETLDGEHVEL
                     HANIELPEDVTAALANGAAGIGLFRSEFLFLNRPGLPTEDEQFEAYRKAAAGMQGKPV
                     TIRTFDLGADKQPEQFDGLARVAPNPALGLRAVRFCLAEPRLFLTQLRAILRATHYGN
                     VRILIPMLASVSEIDQTLALIARAKETLREQDIPFNPDVKVGGMIEIPAAVLTIGAFL
                     RRLDFLSIGTNDLIQYTLAVDRADEAVSHLYDPLHPAVLRLIGLAIGSATRANVPIAV
                     CGEMAGEMPLTRLLLGLGLREFSMHPSQLLGVKQRVLQTDVGAAAPLIERIRRADDPD
                     RLAALLDKLNA"
     gene            complement(6837..7106)
                     /locus_tag="VFO53_02445"
     CDS             complement(6837..7106)
                     /locus_tag="VFO53_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET8875164.1"
                     /translation="MLRREIEIVNKLGLHARASAKLTQLAAKFDSDVQVERNGRKVNA
                     KSIMGVMMLAAGKGSRITLEVAGADEAQAMEALCSLVSDCFGEGQ"
     gene            complement(7257..>7622)
                     /locus_tag="VFO53_02450"
     CDS             complement(7257..>7622)
                     /locus_tag="VFO53_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HET8875165.1"
                     /translation="IVAHGALGDSLVSAVTHVLGARPPQFDVFPVAADDDPLALLPRA
                     RAAVAVLDTGEGVAVLADLYGATPCNLAVKLAAPGTVEVIAGVNLPMLVRAFTYRTRG
                     MDTLVKKAISGGCEGVVHV"
BASE COUNT         1251 a         2543 c         2667 g         1161 t
ORIGIN      
        1 cgagcagcac gagctgcgtg acgagcatcc agccgcggcg gcggccgagc agcggcagcg
       61 cgtagcgatc gagcagcggc gaccagagga acttccacgt gtacggaatc tggatcagcg
      121 caaagaagcc gatcgtcttc aggtcgacgc cgcccgtacg aagccaggcg ggcagcaggc
      181 tgatcaaaag gtagagcggc agtcccgacg agaagccggt gaagacgcag atcagcatcc
      241 gccggttgaa gagcgcctcg cgccaggttt cgcgatgctc gtcgcggatc atcggcgaaa
      301 ccggtagatg gcgagctcgc cggtcaggtt cggcgcgaag ccgaccgcgc gacccttggc
      361 gaggatcacg cgatcgagaa cccggtagcc gcgctccgcg aggaacgcgt cgaaatcggc
      421 gacggtgcaa aggtggatgt tcggcgtgtc gtaccactgg tagggcagcg acgacgatac
      481 cggcatgcgt ccgcgcagga tctgcagcct gtgcgtccag tggccgaagt tcggaaaggt
      541 gacgatcgcc tcgcggccga cgcgcagcat ttcgccgacg atcgcttcga tgcggcgcat
      601 cgcctgcagc gtctgcgaca ggatcacgac gtcgaacgcg ccgtcgtcga acccggcgag
      661 cccgcgctcg aggtcgctct ggatcacgtt gatgccgttg cgcacgcacg cgaggacgcc
      721 cgcgtcgtcg atctcgatgc cgtagccgct cgcggcgcgc tgcgcggcga gaaacgcgag
      781 caattcgccg tcgccgcaac cgaggtcgag cacgtgcgcc tgctggccga cccagccgcc
      841 gatcgtcgcg tagtcggcgc gcgcgacgcc attgacggaa atcggggtca gagccgtatc
      901 ttcctgccgg acgccggtca tgccgaaaac gcgttcggag atcgagctct gaccccggct
      961 tccccgatgt cgccgatctc cgacgccatc cgctcgaacc atgcgcgcac tacggcgtga
     1021 tactgcgcgt tgtcgagcag gaacgcatcg tggccgtgcg gcgccgcgat ttcggcgtac
     1081 gagacgtcgc ggcgctttcg caccagcgcc ttgacgattt cgcgcgaacg cgaggcggga
     1141 aagcgccagt cggtcgtgaa cgacacgacg agaaagcggc acgtcgccgg tcccagcgcg
     1201 cgcgccaggt cgccgccttc gcgcgtggcc ggatcgaaat agtcgagcgc cttcgtcacg
     1261 cgaagatagg tgttcgcgtc gtagtattcg gcgaacttct cgccctgata gcgcaggtac
     1321 gactcgatct ggaattcggg tgcgaacgag aagttgagcc cgtcgcgcag ctggcgcccg
     1381 aacttggcct ccatctgctc gtcggacaga taggtgatgt ggccgatcat ccgcgccacg
     1441 cgcaaccctc gccgcggctt cacgccgtgc gcgccgaagt tgccgtcgtg gtattcggga
     1501 tcggtcatga tcgcctggcg cgcgatctcg ttgaacgcga tgttctcggc cgacaggttc
     1561 ggcgcggcgg cgatcacgag cgcattgcgg atgcgcgacg gatagcggat cgcccagtcg
     1621 agcgcctgca tgccgccgag gctgccgccc attaccgccg cccattgccg gatgccgagc
     1681 cgatcggcga gctgcgcctg cgcgtcgacc cagtcctcga cggtcacgat cggaaagtcg
     1741 ctgccccagg gctcgccggt cgccgggttc gatgaaaccg gtccggtcga gccgaagcag
     1801 cttccgagat tgttcatgcc gacgacgaag tagcgcccgg tgtcgagcgg cttccccggc
     1861 cccaccatgt tgtcccacca gccgatgttg tcgggatcgt cggcgtagaa gccggcaacg
     1921 tggtgcgacg cgttcagcgc atgacagatg agaacggcat tcgagcgcga cgcatcgagc
     1981 gtcccatacg tctcgtacgc gaggtcgtac tggggaagcg tgcggccgca ggcgagcggc
     2041 agtggcgcgt cgaagtgcgc gacttgcggc aggacggggc cgacggagcg ggcggcggcc
     2101 gcgggcgcat cggcgctcgc ggtcgtgtcg gtgaacgatg gcatcgacgc gcatcgtagc
     2161 gcaacggcgt cgtggcgcac gccgccagcc aacgttcgcc gcacgcgcaa cgcgtgccgc
     2221 ggccggcttt accgcgcgca ggacacgacg aatgcgggcg ccgcgcagct tgcgtcacgc
     2281 accgtcaccg atccatcctg tgccaccatc aggaacgcaa cgccgagcgg cgccaggaac
     2341 ggctcggcgt cacgctcgag cagcatcgcg atagtcgcgt agctcccggc gaggatcgca
     2401 agcggcgccg cgacgctgac cgattgccac tgcgaaacgg gcaggcctgt ccgtggatcg
     2461 agcagatgac aatagcgccg cccgccgact tcgaagtaac gctcgtaatc gccgctggtg
     2521 gcgacggccg cgtcgtcgac gaggatgctc gcgatcgtcg cgtcctcgcg tcgcggatcg
     2581 cgaatgccga cgcaccatgg cgagccgtcg gcacgcggtc cgatcgcacg cacgtcgccg
     2641 ccgaggttga cgagcgcatg cacgatgccg aggtcgcggc agatgacggc ggcgcgatcc
     2701 gccgcgtatt ccttgccgat gccgccgaag tcgagctcca tgccggcgtg cggcaagcgg
     2761 acggcggcat cgctccattc gacgcgatcc cacccgatca gctcgcgcgc cgccgcaagc
     2821 gttgcatgcg atggaacgcg cggcgggcgg cgcttgaagt cccacgcgcg acgcagcacg
     2881 cccgacgtga tgtcgaagcg gccgccgctt tgcgcgtgac accggtcggc gtagcgcagg
     2941 agcgccgcgg tctcggcgtc gatcgccacg gccgcccggc ccgcggcgcg gttgatcgcc
     3001 gaggtgacgc tgtcgtcgcg atagcgcgaa tacttggact cgatgcgccg cacgtctgcg
     3061 atcgctgcgg cggcgacgcg atcggcgagt gcccggtcgg gcgtcgacag gtgcagctcg
     3121 tgcacggacg ccatcgcggt gaagcggtag cgaaatggcg caggcggggc gtcgcgatgc
     3181 gtcagaagcg cttcgacagc ccgagctcga tccatcgcgc ggagaattcg gcgagattcg
     3241 cgctgccacc gccgatgcgc cagtcgccgc gctgccgata gacgtcgatg gcgagatcgg
     3301 ccgtgacgcc gtgtggaagc tccttcgcca ggcgcaaccc gagggtcacg gcgccgaacg
     3361 ccgagagccg cgcgtcggcc gtgtacaact ggcccggcac gaagccttcg ggaaacggcg
     3421 gatcgcgata gaagcttgcg gccgattgcg tgaggtagcg cagcgtcgga gtcagcgtga
     3481 gcccgtgcgg aagcgcctgc acccacgacg cctcgagcat gtgcgagcgg tcgccgaacg
     3541 agtcgtcgag atagcgatac gcgagccgca gcgttgcatc cggtgcgggg aaataccggt
     3601 tgtagtgcgt gagccacacg aggatctgcc gccggttcgg gcgggtgtcg tgcagcttgt
     3661 acggatcggc gtagtaaccg cggccgccgg accacgtgag gctcgattcg accagcgacc
     3721 gggcgtcgag cacctgcgtg atgccgagca ggtaatcgta cgtttcgcgc ggccgatcga
     3781 gcaccggccc gttgacgcct tcgggcgcgt tgatccgatc cttcgcggca ccgacgccga
     3841 ccgcatacgt gcggttgcga tcggcgctcc acgtgcgcca ctcgaccgac gcggcgcgcg
     3901 agatgtaatc ctgctcgcgg gaataggcag cgcccagtgc cagcgcggaa ctgccgaagt
     3961 accgcgtcag cttcgcatcg gccgcgctgc ggtagtcgcc gatgccgcgc ccggacgcac
     4021 ccgacaccgt gtcgtgatag agcggcgacg cgccggaaac gctgtcgtag acgagcgagc
     4081 cttccacggt gagcgtgtcg gacagcggaa cgagcgcata cagcgacgga ctgcggaccg
     4141 atatgcgatc cgcacccggc tgccagtcgc ggtaatcgta atagcgaagc gcgatcacgc
     4201 cttcgtcggg tgcgctttgc gcaagcgacg tcgctggcag catgccgggc agcgcgagcg
     4261 ccgccgcgat cagtgccgcc gaccgatcgg tgctcgtgtc cgcattcgcg cgtcgccttg
     4321 ccgcggacag cggaaactcc tcagttgcag ccacacccgc cgccgccgac gctgccgccg
     4381 ccgcccgcgc cctccttgct ctggtagatg tgctgcgtga gcttcgcctc gagcggatcg
     4441 ctgtcgaagc gcatcgacga tcgcgcgaga tcgcccttct cccacggttt cggcggagtc
     4501 atcgccgcgc acccggcgag ggccgcgatc gcgccggccg caagcaagcg ctcaatgctg
     4561 cgcgagcaat gcacggatgc gggcctcgag tgctgcgcgg cgctcgtcgt gaaagccttc
     4621 ctcgaccgcg gcgatattgc cctggcgatc gatcaggtac gagctcggca tgccccgcac
     4681 gtcgtaggcg gagggcgtcg tgccttcggc gtcgtaggcg acggtgaacg atgccggcgt
     4741 gtcgcgcagg aaccgttgcg catcgacgcg gcgcttgtcc acgttgacgc cgacgacggt
     4801 gagcccttcg ccggcgtagc gcgcctgcat cgcgttcatc cacggaaagg atctccggca
     4861 cggcgtgcac cacgacgccc agaaatcgac gtagacgacg cgcccgcgaa gcgctgcgag
     4921 cgccaccgtt tcgccgctcg ccgtcgagag tgcgaacggc ggagccggcg tgccgggatt
     4981 cgccgcgtgg gtcgatgctg cgcaggtcag gccggcgagc aggaaagcga ggaggaggcg
     5041 catcggaaag ccgagtgact gcctcgcgca aacaacgcct gtcgggccgc tattccgttc
     5101 aggcgttgag cttgtcgagc agtgctgcga gccggtcggg atcgtcggcg cgccgaatgc
     5161 gctcgatcag cggcgccgcg gcgcccacat cggtctgcag cacgcgctgc ttgacgccga
     5221 ggagctgcga cggatgcatc gagaattcgc gcaatccgag tccgagcagg agccgcgtca
     5281 gcggcatctc gccggccatc tcgccgcaga cggcaatcgg cacgtttgcc cgggtggcgc
     5341 tgccgatcgc aagcccgatc aggcgcagca ccgccggatg cagcggatcg tacagatgcg
     5401 acaccgcttc gtcggcgcgg tcgacggcga gcgtgtactg gatgagatcg ttggttccga
     5461 tcgaaaggaa atcgagcctg cgcaggaagg cgccgatcgt cagcaccgcg gccgggattt
     5521 cgatcatgcc accgaccttc acgtcggggt tgaatggaat gtcctgctcg cgcagcgttt
     5581 ccttcgcgcg ggcgatgagc gcgagcgtct ggtcgatctc ggacaccgac gcgagcatcg
     5641 ggatcaggat gcgcacgttg ccgtagtgcg tggcgcgcag gatcgctcga agctgggtga
     5701 ggaagagccg cggctcggcg aggcagaagc gcacggcgcg aaggccgagc gcaggattgg
     5761 gcgcgacgcg cgcgaggccg tcgaattgct cgggctgctt gtcggcgccg aggtcgaacg
     5821 tgcggatcgt caccggcttg ccctgcatgc cggccgcggc cttgcgatac gcctcgaact
     5881 gctcgtcctc ggtgggcaag cccggccggt tgaggaacag gaactcggag cggaacaagc
     5941 cgatgcccgc ggcgccgttg gcgagcgccg cggtgacgtc ctcgggcagc tcgatgttcg
     6001 cgtgcagctc gacatgctcg ccgtcgagcg tctcggcgcg cttgctgcgc agccgcttga
     6061 gcttctggcg cgcgagatcg aactcatcct gcttcagccg gtattcggcg agcacggcgc
     6121 gatcgggatt gacgatgacc acgtggtggg tgccgtcgac gatcagcatc tcgccgtcgc
     6181 gtatcagctg gcgcgcgttg tgcgtcgcga cgacggcagg cacgttgagg ctgcgcgcca
     6241 cgatggccgt gtgcgacgtg acgccgccga ggtcggtgag gaacgccgcg aaatggtggt
     6301 gcttgaactg gatgacgtcg gccggcgaga ggtcgtgcgc gacgagaatt gtctgctcct
     6361 cggcgctcgg cgcgggcagc gtgccggggc ggcccatgag ccgcttcagc acgcgctcgc
     6421 cgacctgcac gacatcggcc ttccgctcgc gcagataggc atcctcgatc tggtcgaact
     6481 gctcgacgag cacgcccatc tggcgcgcga gcgcccactc ggcgttgcag ctctgctcgg
     6541 cgatgatccg cttgggtgcc tccgacagcg tcggatcgtt caggatcatc caatgcacgt
     6601 cgaggaaggc gccgaactcg ccgggaacgt cgccgctcgt cagcgcggcg tgcagccctt
     6661 cgagctcgcg ctgcacgtcc ttgaccgcgt tttcgaagcg cgcgatctcc gacggcacct
     6721 gctggtcgcg gatcgtgtaa tgcgaaactt cacgtgtcgc atgcgagacg agctgcgcgc
     6781 ggccgatcgc gatgccgccg gtcacgccga tgccgtgaag cgcgaagctc accaacctac
     6841 tgtccctcgc cgaagcagtc gctgacgagc gagcacagtg cctccatcgc ctgcgcctcg
     6901 tcggcgccgg ccacttcgag cgtgatcctc gatcccttcc cggccgcaag catcatgacc
     6961 cccatgatgc tcttcgcgtt caccttgcgg ccgttgcgct cgacctgcac gtcactgtcg
     7021 aacttcgcgg cgagttgcgt gagcttcgcc gacgctcgtg cgtgcagccc gagcttgttg
     7081 acgatctcga tttcgcggcg aagcatcgtt attcagccgg gaagggccgc cccgaccggc
     7141 tgaaccgccc cccggggggc agcgagcgcg tgagcccgac gggccgcccc gagggcgaac
     7201 acgcgagcgc gaagcgcgag gatcgtccaa ccgagcgtgg ggggatgttc catccttcag
     7261 acgtggacga cgccttcgca accgcccgag atggccttct tcacgagcgt atccattccg
     7321 cgcgtgcgat aggtgaacgc gcgcaccagc atcggcaggt tcacgccggc gatcacttcg
     7381 acggtgccgg gcgcggcaag cttgaccgcg agattgcagg gcgtggcgcc gtaaagatcg
     7441 gcgagcaccg cgacgccttc gccggtgtcg agcaccgcca ccgcggcgcg cgctctcggc
     7501 agcagcgcga gcggatcgtc gtcggccgcg acgggaaaca cgtcgaactg tggcggccgc
     7561 gcgccgagca cgtgcgtgac cgcggagacg agactgtcgc cgagcgcgcc gtgcgcgacg
     7621 at
//
LOCUS       DASSVX010000052         7167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_618426, whole genome shotgun sequence.
ACCESSION   DASSVX010000052 DASSVX010000000
VERSION     DASSVX010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618426"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFO53_02455"
     CDS             <1..282
                     /locus_tag="VFO53_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET8875166.1"
                     /translation="TPRGGRIDVSVERDEHGVTLGVADTGPGIPAEARERVFDRFYRG
                     DDNAEPGSGLGLSIVRRIADAHHASITLDAAGEGSGLVVGVHFPREPDS"
     gene            364..1842
                     /locus_tag="VFO53_02460"
     CDS             364..1842
                     /locus_tag="VFO53_02460"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET8875167.1"
                     /translation="MQAKFHLKQITVALAAAGFGLAVGAGYNRLDVPALSLANAATPP
                     AITSPVASTAAARLPDFTALVERDGAAVVNISTVGTTKTTGLDQDDEDNPLNEFLKRF
                     GMPNGPGLNIQPRAVPSRGVGSGFIVSPDGYIVTNAHVVDGANTVTVKLTDRREFTAK
                     VIGTDKRTDIALIKIDSKKPLPALDLSNPAPAKQGEWVIAIGSPFGFENSVTAGIVSG
                     VHRALPGGQMTPFIQTDVAVNPGNSGGPLLNTAGQIVGVNSQIYSRSGGFMGLSFAIP
                     ASVAQNVAMQLKEHGKVNHGRLGIGIQGLDQTLAESFNLPDSNGALVGTVEENSPAAK
                     AGFRTGDVIRKIDNEPVGDSTDVTSRIGNMAPGTKVNVEVWRDGKPVALTATIGSFDS
                     AEKVASNDKSSLSHEKLGLAVRPLSPDEKQEVGHGGLVVENASGPAALAGIQPGDVIV
                     SVGSAKVTTVEGLKQQVDKAGKNIALLIERNGQKIFVPVRVG"
     gene            2167..3645
                     /locus_tag="VFO53_02465"
     CDS             2167..3645
                     /locus_tag="VFO53_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET8875168.1"
                     /translation="MRFRNMLMLAGIAGFGATSVAHADDVSDMKAQMEALQKQLDAVK
                     TQLYNMQEAKKKEEVAEKERPFIRMKPNAGFTFEVGKGEVQIYGHADLSVDEVDNGLK
                     NRFGAQGKMGWLLQEASNLSYFGVRGNRELIPGITGLFQFETEVAYVNTPGPTSDAQI
                     KQGLGSRNSFVGIEGAFGAVKLGKTDAPYKTSTSRMDPFSSSVGDYNSILGNSGGDNR
                     AEFDTRVPHSVWYESPKFNGFAFNLLFSPGQNRSDDNSILPRGEPICAGGNGEPCGDG
                     SFGDLWSTSLVYSQGPWYAIVAYEHHAKVNRTGDEGGSFVPGIPPPGAVGVADESAVK
                     VGVQYQFKQTGTTINAIYERLHRNAPDDNFNERTRHTATWLALTQKITPADDFNIGWA
                     HAGRTSGDPGAAINATDSLANFAGPIDNEANMYAIGYKHHFADNRTTWYAVFAEQRNH
                     VGAHYDLGASGHGNVVDCKDATGACFAGAILKAISVGITYDF"
     gene            3773..4444
                     /locus_tag="VFO53_02470"
     CDS             3773..4444
                     /locus_tag="VFO53_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8875169.1"
                     /translation="MRILVVEDDPLLAESLLRALQQQGYGVGHARRGHDADLLLHNHR
                     FDLLLLDIGLPDFDGFEILRRLRARADEIPVLVVTAREAVDERVHGLDLGADDYLTKP
                     FSLSELEARVRALLRRAKPALAPRIVAGNLVVESAAKRARIDGKPVELTAREWTLLEL
                     FLTRPGHALSKLTIAKTLGDSDTAIAPNTVEVYVSRLRAKLEPAGIVIRTVRGFGYLW
                     EQPHA"
     gene            4437..5879
                     /locus_tag="VFO53_02475"
     CDS             4437..5879
                     /locus_tag="VFO53_02475"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET8875170.1"
                     /translation="MREAEGAGSIQARLLKVLMVSLVALLVLSGSVTWFVAYRVANDA
                     YDHALLDPLMDIVQNVRQGADGPALSLSPREQEALLFDGSDRVFFQVRGPAGIWFAGG
                     ARDIPEAAEPLVPRVPVFYSTSVAGSAVRIAAMRTDGGFEVYVAETTNKRDRLVWEII
                     LTGLVPLLLVVVVAVVLVWFGVSHGLAPLRKLRGELSQRSPNDLRAVDESHAPSEVRP
                     AIIALNRLFQRIHETSESQRRFLADAAHQLRTPLAGLQMQLELGLRASQSEADRATLA
                     KMRDAVLRTGKLTNRLLALARAEQAVQDRQEFRRVDLPAVCERVGGQWIARAIDRNID
                     LGFELASGYVDGDEALLADMLDNLIDNALRYTRPGGAVTVRCGADAAGVFFGVEDNGA
                     GIPEAERGRVFERFYRGEGVGGDGSGLGLAIVKEGAGRHDAAVGIGVPPNGAGTWVRV
                     TFGRPADASAVAASTASRAYAPAAIRVGTA"
     gene            complement(5920..6519)
                     /locus_tag="VFO53_02480"
     CDS             complement(5920..6519)
                     /locus_tag="VFO53_02480"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET8875171.1"
                     /translation="MAERFIAPVTLQGRHATLEPLAPAHLDEIRAAASDGELWRLWYT
                     SVPAPAHAPAWLAATLEMREKQGALPFVVRDNTDGSLVGSTRYFNVDAANRRLEIGHT
                     WYAKRAQRTAINTECKLLLLTHAFETLRCIAVEFRTHWFNQASRIAIARLGAKQDGVL
                     RNHQLMPDGSRRDTVVFSIVDGEWLAVKQHLRFLLERPR"
     gene            complement(6551..6886)
                     /locus_tag="VFO53_02485"
     CDS             complement(6551..6886)
                     /locus_tag="VFO53_02485"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET8875172.1"
                     /translation="MDGALKLWSVVAIVGVLNYLSRVSFIAIFARRHVPVLLARAFRY
                     VPAAMLTALVLPMVVAAPGAPVLASAPKIVAAAVAGAVAFVTHSTLKTLATGMLTLWA
                     LQLAGSAFG"
     gene            complement(6871..>7167)
                     /locus_tag="VFO53_02490"
     CDS             complement(6871..>7167)
                     /locus_tag="VFO53_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8875173.1"
                     /translation="GSGFALWAFWQLTNIAGYFVGNLIPPAWSLDFAVPLCFIALVAP
                     HFRTSPTIVAAVVAGVGVLALAALPMKLNLIVAGVAGIAAGTLADFAKERWTAR"
BASE COUNT         1261 a         2393 c         2403 g         1110 t
ORIGIN      
        1 acgccgcgcg gcggacgcat cgacgtgtcg gtcgagcgtg acgaacacgg cgtcacgctc
       61 ggtgtcgccg acaccggtcc gggcatcccg gccgaggcgc gcgaacgcgt cttcgaccgc
      121 ttctatcgcg gcgacgacaa cgccgagccc ggcagcggcc tcggactatc gatcgtccgg
      181 cgcatcgccg acgcgcatca cgcgtcgatc acgctcgacg cggccggcga gggcagcggg
      241 ctcgtggtcg gcgtgcactt tccgcgcgaa ccggactctt aaggtcacgt taagcgccgt
      301 tccgtatatt cagcgtgccg gcctcgcatg aagaggtccc ctcgttcccc aaggagtgca
      361 tcgatgcaag cgaaattcca cctgaagcag atcaccgtcg cgcttgcggc tgcgggcttc
      421 ggcctcgccg tcggcgcggg ctacaaccgg ctcgacgtgc ccgcgctgtc gctcgccaat
      481 gccgcgacgc caccggccat cacgtcgccg gttgcctcga ccgcggccgc acggctgccc
      541 gatttcacgg cgctcgtcga gcgtgacggg gcggccgtcg tcaacatcag caccgtcggc
      601 accacgaaga ccaccgggct cgaccaggac gacgaagaca atccgctgaa cgaattcctc
      661 aagcgcttcg gcatgccgaa cgggcctggg ctcaatatcc agccgcgcgc cgttccgtcg
      721 cgtggcgtcg gctcggggtt catcgtgagt cctgacggct acatcgtcac caacgcacac
      781 gtcgtcgacg gcgcgaacac cgttacggtg aagctcaccg accgccgcga attcacggcg
      841 aaggtgatcg gcaccgacaa gcgcaccgac atcgcgctga tcaagatcga ctcgaagaag
      901 ccgctgccgg cgctcgacct gtctaacccc gcacccgcga agcagggcga atgggtgatc
      961 gcgatcggct cgccgttcgg cttcgagaac agcgtgacgg ccggcatcgt gagcggtgtg
     1021 catcgcgcac tgcccggcgg ccagatgacg ccgttcatcc agaccgacgt cgccgtcaat
     1081 cccggcaatt cgggcggccc gctgctcaac accgctggcc agatcgttgg cgtcaactcg
     1141 cagatctaca gccgctcggg cggattcatg gggctgtcgt tcgcgattcc cgcgagcgtc
     1201 gcgcaaaacg tcgcgatgca gctcaaggag cacggcaagg tcaaccatgg gcgcctcggc
     1261 atcggcatcc agggtctcga ccagacgctc gccgagagct tcaacctgcc cgattcgaac
     1321 ggcgcgctcg tcggtaccgt cgaggagaac agcccggcgg ccaaggccgg cttcaggacg
     1381 ggcgacgtca tccgaaagat cgacaacgag ccggtcggcg acagcaccga cgtcacgagc
     1441 cgcatcggca acatggcgcc cgggacgaag gtcaacgtcg aagtctggcg cgatggcaag
     1501 ccggtggcgc tcaccgcgac gatcggctcg ttcgacagcg ccgagaaggt cgcgagcaac
     1561 gacaagtcgt cgctgtcgca cgagaagctc ggcctcgccg tgcggccgct ttcgcccgac
     1621 gaaaaacagg aagtcggcca tggcggactc gtcgtcgaga atgcgagcgg tcccgcggca
     1681 ctggccggca tccagccggg tgacgtgatc gtcagcgtcg gttcggcgaa ggtcacgacg
     1741 gtcgaaggcc tgaagcagca ggtcgacaag gccggcaaga acatcgcgct gctgatcgag
     1801 cgcaacggcc agaagatttt cgtgccggtt cgcgtcggct gatcgagcta tcggcacccg
     1861 gcggccgccg ggtgcatcga aagggccgcg gatttccgcg gccctttttc gttggcggta
     1921 ccaggccatg cgtgatggcg cgtcggccag cggaatccga gccgatgtca gcgaaatgtc
     1981 aggaccgcgt cagccacctg caagacttcg ccggacggtg tggcgttcct gtcatcgatg
     2041 cgcgcgaacg ccgcggcagc acatgcgcgt cgcgttgcct ttcctccggg cacggatcaa
     2101 cgttgaagca aggccgcgag agccactgcg cggcgcgcag caaccattcc cgttcgagga
     2161 ggaagtatgc ggtttcggaa catgctgatg ctggccggca ttgccgggtt cggcgcgacg
     2221 agcgtcgcgc acgccgacga cgtaagcgat atgaaagcgc agatggaagc gctgcagaag
     2281 cagctggacg ccgtcaagac acagctatac aacatgcaag aggccaagaa gaaagaggaa
     2341 gtagccgaga aggaaaggcc gttcatccgc atgaagccca atgccgggtt cacgttcgaa
     2401 gtcggcaagg gcgaagtgca aatttacggc cacgccgatc tgtccgtcga cgaggtcgac
     2461 aacggcctga agaaccgctt cggcgcgcag ggcaagatgg gctggcttct gcaggaagcg
     2521 agcaatcttt cctatttcgg cgtccgcggc aatcgcgaac tcatcccggg catcaccggc
     2581 ctcttccagt tcgaaaccga ggtggcctac gtgaacacgc ccggaccgac gagcgacgcg
     2641 cagatcaagc agggactggg ttcgcgcaac agcttcgtcg ggatcgaggg cgcattcggt
     2701 gccgtgaagc tcggcaagac cgacgcgccg tacaagacgt cgacgtcccg aatggatccg
     2761 ttctcgtcga gcgtcggtga ttacaacagc atcctcggaa acagcggcgg cgacaaccgt
     2821 gccgagttcg acacgcgcgt gccgcactcc gtgtggtacg agtcgccgaa gttcaacggc
     2881 ttcgcattta atctgctgtt ctcgccgggg cagaaccgct cggacgacaa cagcattctt
     2941 ccgcgcggcg agccgatctg tgcgggcggc aatggcgagc cctgcggcga cggatcgttc
     3001 ggcgacctgt ggagcacgtc gctcgtctat tcacaggggc cctggtacgc gatcgtggcg
     3061 tacgagcacc atgcgaaagt gaatcgcacc ggcgacgaag gcgggtcctt cgtccccggc
     3121 attccgccac ccggcgcagt cggcgttgcg gacgaaagcg cggtcaaggt cggcgtgcag
     3181 taccagttca agcaaaccgg caccacgatc aacgcgatct acgagcgcct gcaccgcaac
     3241 gcgcccgacg acaacttcaa cgagcgcacg cggcatacgg ccacctggct cgcgctgacg
     3301 cagaagatca cgccggcgga cgatttcaac attggctggg cgcacgcggg ccggacgtcg
     3361 ggcgatccgg gcgcggcgat caacgcaacc gacagcctcg cgaacttcgc cggcccgatc
     3421 gacaacgaag ccaacatgta cgcgatcggc tacaagcacc acttcgccga caaccgcacg
     3481 acgtggtacg cggtcttcgc cgagcagagg aatcacgtcg gtgcgcatta cgatctcggt
     3541 gccagcggac acggcaacgt cgtcgactgc aaggacgcga ccggcgcctg ttttgccggc
     3601 gctatactga aagcgatctc cgtcggcatc acctacgact tctagctctc ctcgccgcgc
     3661 gcccgcctcc tccgcgggtg cgcggttgca gcaaggcagg ggtggcggcg gcgatctttg
     3721 ccgccgccac gtcggcctct tgtcacggtg atcgcggccg gaagggacgg ccttgcgcat
     3781 cctcgtcgtc gaagacgatc cgctgcttgc cgaaagcctg cttcgcgcat tgcagcagca
     3841 gggttacggt gtcgggcacg cgcggcgcgg tcacgacgcc gatttgctgc tgcacaatca
     3901 ccgcttcgac ctgctgctgc tcgacatcgg tctgccggac ttcgatggat tcgaaatact
     3961 gcgacgcctc cgggcccgag ccgatgaaat accggtgctc gtcgtgaccg cacgcgaagc
     4021 ggtggacgag cgcgtgcacg gcctcgatct cggcgccgac gactacctga cgaagccgtt
     4081 ctccctttcc gagctcgagg cgcgcgtgcg ggcgctgctg cgccgcgcga agccggcgct
     4141 tgcgccgcgg atcgttgccg gcaacctggt cgtcgaaagc gcggcgaagc gggcgcggat
     4201 cgatggcaag ccggtcgagc tgaccgcgcg cgagtggacg ctgctcgagc tcttcctcac
     4261 tcgtcccggc cacgcgttgt cgaagctcac gatcgcgaag acgctcggcg acagcgacac
     4321 ggcgatcgcg ccgaataccg tcgaagtgta tgtatcgcgt cttcgcgcga agctcgagcc
     4381 cgccggcatc gtcattcgga ccgtgcgcgg cttcggctat ctgtgggagc agccgcatgc
     4441 gtgaggcgga aggcgccgga agcattcagg cgcgcctgct gaaggtgctg atggtgtcgc
     4501 tcgtcgccct gctcgtgctg agcggcagcg tcacgtggtt tgtcgcgtat cgcgttgcga
     4561 acgacgccta cgaccatgcg ctgctcgatc cgttgatgga catcgtgcag aacgtgcgcc
     4621 agggtgccga cggtcccgcg ctgtcgctgt cgccgcgcga gcaggaggcg cttcttttcg
     4681 acggctccga ccgcgtcttc ttccaggtgc gtgggccagc cggaatatgg ttcgccggcg
     4741 gtgcgcgcga tattccggag gcggcggaac cactcgtccc tcgcgtgccg gtgttctaca
     4801 gcacgagcgt cgcaggaagc gcggtgcgca tcgccgcgat gcgcaccgac ggcggcttcg
     4861 aggtctacgt ggccgagacg acgaacaagc gcgaccgcct cgtgtgggaa atcattctca
     4921 ccggtctcgt gccgctgctg ctcgtcgtcg tcgtcgccgt cgtgctggtc tggttcggcg
     4981 tgagccacgg gctcgcgccg ctacggaagc tgcgcggcga gctgtcgcag cgctcgccga
     5041 acgaccttcg cgccgtcgac gagtcgcacg cgccttccga ggtgcgcccc gcgatcatcg
     5101 cgctgaaccg gttgttccag cggatccacg aaaccagcga atcgcagcgc cgctttctcg
     5161 ccgatgccgc acatcagctg cgtacccccc tcgccggact gcagatgcag ctcgagctcg
     5221 gcctccgagc atcgcagtcc gaggccgacc gtgcgacgct cgccaaaatg cgcgacgcgg
     5281 tgctgcgcac gggcaagctc acgaaccggc tcctggcgct cgcgcgtgcg gagcaggcgg
     5341 tgcaggaccg gcaggaattc aggagggtcg atctgcccgc ggtgtgtgaa cgcgtgggcg
     5401 gccagtggat agcgcgcgcc atcgaccgca acatcgatct cggcttcgag ctcgccagcg
     5461 gatacgtcga tggcgacgag gcgcttctcg ccgacatgct cgacaacctc atcgacaatg
     5521 cgttgcgcta cacacggccc ggcggcgcag tcaccgttcg ctgcggcgcc gatgccgcgg
     5581 gcgtcttttt cggcgtcgag gacaacggtg ccggcattcc cgaagccgag cgcggacgcg
     5641 tgttcgagcg gttctatcgc ggcgaagggg tcgggggcga cggctcgggg ctcggccttg
     5701 cgatcgtcaa ggaaggcgcg ggccgccacg acgccgcggt cggcatcggc gtgccgccga
     5761 acggcgccgg gacgtgggtg cgcgttacgt tcggccgacc ggccgatgcg tcggccgttg
     5821 ccgcatcgac cgcctcacgc gcgtacgcac cggctgccat ccgggtcggc accgcgtagc
     5881 agcgatgcgt cgggggccga tcgcggccac gccgatgcgt cagcgcggcc gctcgagcaa
     5941 aaagcgcaga tgctgcttca ccgcgagcca ttcgccgtcg acgatgctga atacgacggt
     6001 gtcgcggcgg ctgccgtcgg gcatcaactg atggttgcgc agcaccccgt cctgctttgc
     6061 gccgagccgc gcgatcgcga tgcgcgacgc ctggttgaac cagtgtgtcc ggaattcgac
     6121 ggcgatgcag cgcagcgtct cgaatgcgtg cgtcaacagc agcagcttgc actcggtgtt
     6181 gatcgccgtg cgctgcgcgc gcttcgcata ccacgtgtgg ccgatctcga gccggcggtt
     6241 ggcagcgtcg acgttgaaat agcgcgtgct gccgacgagg ctgccgtccg tgttgtcgcg
     6301 cacgacgaac ggcagcgcgc cctgcttctc gcgcatctcg agcgtggcgg cgagccaggc
     6361 gggtgcgtgc gccggcgcgg gcaccgatgt gtaccagagg cgccatagtt caccatccga
     6421 cgccgcggcg cgaatttcgt cgaggtgcgc gggggcaagc ggttcgaggg ttgcgtgccg
     6481 gccctgcaac gtgaccggcg cgatgaagcg ctctgccatg cgcgttctcg ttcgtggatg
     6541 aaatacgatc ttagccgaat gcggagccgg cgagctgcag tgcccacaac gtgagcatgc
     6601 ccgttgcgag cgtcttcaac gtgctgtgcg tcacgaacgc cacggcgccc gcgacggcgg
     6661 ccgcgacgat cttcggcgcc gacgcgagca ccggcgcacc cggcgccgcg acgaccatcg
     6721 gcagaaccag cgccgtcagc atcgccgccg gcacatagcg aaatgcgcgc gcgagcagta
     6781 ccggcacgtg ccggcgcgcg aagatcgcga tgaacgacac gcgcgacagg tagttcagca
     6841 cgccgacgat cgcgaccacc gaccagagct tcagcgcgcc gtccatcgct ccttcgcgaa
     6901 atcggcgagc gttccggcgg cgatcccggc gacgccggcg acgatcagat tgagcttcat
     6961 cggcagcgca gccagtgcca gcacgccaac gccggcgacg acggcggcga cgatcgtcgg
     7021 cgacgtgcga aagtgcggcg cgacgagcgc gatgaagcag agcggcacgg caaagtcgag
     7081 cgaccaggcg ggcggaatca ggttgccgac gaaatagccc gcgatgttag tgagctgcca
     7141 gaacgcccac aacgcgaatc cgctgcc
//
LOCUS       DASSVX010000053         4137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_685013, whole genome shotgun sequence.
ACCESSION   DASSVX010000053 DASSVX010000000
VERSION     DASSVX010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685013"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1070)
                     /locus_tag="VFO53_02495"
     CDS             complement(108..1070)
                     /locus_tag="VFO53_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HET8875174.1"
                     /translation="MNLLAIVVALALEQWRSFEWRVSVERGFVRYARGIERRLNGGTL
                     GQGIIATVLAIAPPVLLAALAWWLLDRVHPFFGLVLNVLVLYALMGFRRFSHAISAII
                     SALKSADLGGARRALASWHTASASELSSQDIARLAIERGLADAYRQVFAVLFWFVVLP
                     GPVGAVLYRAAALLASEWKGALPGDDMTVLARSLTVFGRPARLLLWLLDWIPARLTAL
                     SFAVVGDFEDAAFCWRTQARTWPEAYGGEAIGVVLASGAGALGVQVGGSLPGYDGAPD
                     PRPEIGIGDAVEAEILPSAVGLVWRALVLWLLLVFLLSLANWAP"
     gene            1187..2848
                     /locus_tag="VFO53_02500"
     CDS             1187..2848
                     /locus_tag="VFO53_02500"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HET8875175.1"
                     /translation="MTREVMEYDVVIVGGGPAGLAAAIRLKQLAFAASRELTVCILEK
                     GSEIGAHILSGAVIDPRALDELLPDWKAAGAPLVTSVAEDRFLFLTERGAHRIPSRLL
                     PPLMSNHGNYIGSLGDLCRWLAQQAESLGVEIYPGFPATQILYGDRNEVLGVATGDMG
                     VAKDGSHKPGYQEGMELRARYTLFAEGCRGSLSQTLMQRFSLRDGVDPQKYGIGIKEL
                     WEVAPQRHTPGLVMHSIGFPLDNATGGGSFVYHFGDNRVAAGFVVHLDYANPHLSPYD
                     EFQRFKLHPAIRPTLEGGKRIMYGARAINEGGLQSVPSLTFPGGALIGCAAGFVNLPR
                     IKGSHNAMKTGMLAAEAAFAALGDGRAHDELRAYETSWRGSWVYDDLFKVRNVKPGLR
                     FGTLAGTLHGGVHMWLNDLGLGGLAPWTMRHRRADHESLQPAGRMPVIAYPKYDGAVT
                     FDRLSSVYLSNTNHEEDQPVHLQLRDPSVPVDVNLALYDGPEARYCPAGVYEFVADEA
                     GSGKRRLQINAQNCVHCKTCDIKDPQQNIRWVPPEGGGGPNYGSM"
     gene            complement(2918..3814)
                     /locus_tag="VFO53_02505"
     CDS             complement(2918..3814)
                     /locus_tag="VFO53_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HET8875176.1"
                     /translation="MSVRLTASPKPRRQPPRRSSPTMGCVAIKPIPSEEGMRISLKAA
                     VGVVGLVAATTAAAQVTLYSRDGFSGQQFTANGTVHNLDRLGFNDRASSVVVNDGNWQ
                     VCEDADFNGRCVILQPGEYPTLDRIGLENEISSLRPVESRYGANDDRRAYVAQGYDNH
                     RRADERLYEAPVTSVRAVVGPPEERCWIERDEYRGETNVPGAVAGAVIGGLLGNQIGH
                     GSGRAAATAGGAIAGGAIGSNVGTDNGYGQDVQHCRTVGRYDHPDYWDVTYSFRGERH
                     RVQMTSPPGATIAVNADGEPRT"
     gene            complement(3811..>4137)
                     /locus_tag="VFO53_02510"
     CDS             complement(3811..>4137)
                     /locus_tag="VFO53_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET8875177.1"
                     /translation="HEGSEASTQAVVEHAAGGDARCEATLARYEERLARALAHVINIV
                     DPDVVVLGGGLSNIDRWYKSVPLLWTRWVFSDRVDTSLVRNVHGDSSGVRGAAWLSGG
                     SDFRVL"
BASE COUNT          731 a         1408 c         1347 g          651 t
ORIGIN      
        1 cccggcgccg cgaatcggcc cccacgcttg cggcttgcgc cgctgcgctg cccccggagg
       61 gggagcaatt cgcggcttgg gacggcccgg cgccgcgaat ggcgccgcta cggcgcccag
      121 ttcgcgagcg acagcaggaa cacgagcaaa agccacagca ccagcgcccg ccacacgagt
      181 ccgaccgccg acggcaggat ctcggcttcc accgcatcgc cgataccgat ttccggacgc
      241 ggatcgggcg cgccatcgta cccgggcagc gatcctccca cctgcacacc gagggctccg
      301 gcgccacttg cgagcacgac gccgattgcc tcgcccccat acgcttcggg ccatgtgcgc
      361 gcctgcgtgc gccagcagaa cgccgcatcc tcgaagtcgc cgacgacggc gaacgaaagc
      421 gcagtgagcc gcgccggaat ccagtcgagc agccacagca gcaggcgcgc cgggcgtccg
      481 aagactgtca gcgagcgagc gagcaccgtc atgtcgtcgc ccgggagcgc gcccttccac
      541 tccgacgcaa gcagcgccgc ggcgcgataa agcacggcgc cgacgggtcc gggcagcacg
      601 acgaaccaga acagcaccgc gaacacctgc cggtacgcgt cggccaatcc ccgctcgatg
      661 gcaagtcgcg cgatatcctg gctcgacaac tcgctggcgc tcgccgtgtg ccagctcgca
      721 agcgcgcgcc gggcgccgcc gagatcggcg ctcttcaatg ccgagatgat cgccgagatg
      781 gcatggctga agcgccggaa gcccatcagc gcgtagagca cgagcacgtt gaggacgagg
      841 ccgaagaacg gatggacccg gtcgagaagc caccatgcga gtgctgcaag cagcacgggc
      901 ggtgcgatgg cgagcaccgt tgcgatgatg ccctggccga gcgtgccgcc gttgagacgc
      961 cgctcgatac cgcgtgcgta acggacgaac ccgcgctcga ccgacacgcg ccactcgaaa
     1021 gagcgccact gctcgagcgc gagcgcgacg acgatcgcga gcagattcat cccggcacgc
     1081 cgatcggcag cggaacccgc gattcacgcc cgggcaggag catcggcata tgcggacgtc
     1141 attataattg acgcttttca cgcgacgacc cgcgagcggg agtgcgatga cgcgcgaagt
     1201 catggagtac gacgtcgtga tcgtcggcgg cggcccggcc gggcttgcag cagcgatcag
     1261 gctgaagcag cttgccttcg ccgcctcgcg cgagctcacg gtgtgcatcc tcgaaaaggg
     1321 ttccgagatc ggtgcgcaca tcctgtccgg tgcggtgatc gatccgcgcg cactcgacga
     1381 gcttttgccc gactggaagg ccgcgggcgc gccgctcgtc acgtcggttg ccgaagatcg
     1441 gttcctcttc ctgaccgagc gcggcgcgca tcggataccg tcgcgcctgc tgccgccgct
     1501 gatgagcaat cacggcaatt acatcggcag cctcggcgat ctttgccgct ggcttgcgca
     1561 gcaggccgag tcgctcggcg tcgaaatcta tccgggcttt cccgcaacgc agatcctgta
     1621 cggcgatcgc aatgaagtgc ttggcgttgc gacgggtgac atgggtgtcg cgaaggatgg
     1681 atcgcacaag ccggggtacc aggaaggcat ggagctgcgc gcgcgctaca cgctgttcgc
     1741 cgagggctgc cgcggctcgc tgtcgcagac gctgatgcag cggttttcgc tgcgcgacgg
     1801 cgtcgatccg cagaagtacg gcatcggcat caaggagctc tgggaagtcg cgccgcagag
     1861 gcacacgccg ggactggtca tgcacagcat cggctttccg ctcgacaatg caacgggcgg
     1921 cggctcgttc gtctatcact tcggcgacaa ccgcgtcgcg gccggattcg tcgtgcatct
     1981 cgactatgcg aatccccacc tgtccccgta cgacgagttc cagcgcttca agctgcatcc
     2041 cgcaatccgc ccgacgctgg aaggcggcaa gcgcatcatg tacggcgccc gtgcgatcaa
     2101 cgaggggggg ctgcagtcgg tgccatcgct cacgtttccc ggcggcgcgt tgatcggctg
     2161 cgccgcgggc ttcgtcaatc tgccgcgcat caagggttcg cacaatgcga tgaagaccgg
     2221 catgttggcc gccgaagcgg cattcgcggc actcggcgat ggacgcgcgc acgacgaact
     2281 gcgggcgtac gagacatcat ggcgcggctc gtgggtttac gacgatcttt tcaaggtgcg
     2341 caacgtgaag cccggcctgc gcttcggcac gctggccggc acgttgcacg gcggcgtaca
     2401 catgtggctc aacgacctgg ggttgggcgg tctcgcgccg tggacgatgc gtcatcggcg
     2461 cgccgatcac gaatcactgc agccggccgg tcgcatgccg gtcatcgcgt atccgaaata
     2521 cgatggcgcc gtgacgttcg accgcctgtc gtccgtctac ctgtcgaaca cgaaccacga
     2581 ggaagaccag ccggtgcacc ttcagctgcg ggacccgtcg gtaccggtgg atgtgaacct
     2641 cgccctctac gatggtcccg aggcgcgcta ttgcccggcg ggcgtctacg aattcgtcgc
     2701 tgatgaagca ggcagcggga aacgccggct gcagatcaac gcgcagaact gcgtgcactg
     2761 caagacgtgc gacatcaagg atccgcaaca gaacattcgt tgggttccac cggaaggcgg
     2821 cggcggaccg aactacggca gcatgtaacg cgtgcagcag catgcaaaaa ggagcggccg
     2881 catcggccgc tcctcgtgca gcaacgcttt ggcgtgatta cgtgcgcggc tcgccgtcgg
     2941 cgttgactgc gatggtcgcc cccggaggtg acgtcatctg cacccgatgc cgctcgccgc
     3001 ggaacgaata cgtgacatcc cagtaatcgg gatgatcata gcggccgacg gtcctgcagt
     3061 gctgcacgtc ttggccataa ccattgtcgg tgccgacgtt cgacccgatc gcgccacccg
     3121 cgatcgcacc gcctgccgtt gccgcagcgc gacccgaccc gtgcccgatc tggttgccca
     3181 ggaggccacc gatcaccgcg ccggcgacag cgcccggcac gttcgtctcg ccacggtatt
     3241 cgtcgcgctc gatccagcac cgttcctcgg gcgggccgac caccgcacgc accgaggtta
     3301 cgggcgcttc gtacagccgc tcgtcggcgc gccggtgatt gtcgtaaccc tgcgcgacgt
     3361 atgcgcgccg gtcatcgttc gcgccatacc gcgattccac cggccgtagc gacgaaatct
     3421 cgttttcgag gccgatccgg tcgagggtcg gatattcccc gggctgcagg atcacacagc
     3481 gaccgttgaa gtcggcatcc tcgcagacct gccagtttcc gtcattgacc accaccgagc
     3541 tggcgcggtc gttgaagccg agccggtcca gattgtgcac ggtgccgttc gcggtgaatt
     3601 gctgaccgct gaagccgtcg cgcgagtaca acgtcacctg cgccgccgcc gtcgtcgccg
     3661 cgacaaggcc tacgacgcct accgctgcct tgagagagat gcgcatacct tcctccgatg
     3721 ggattggctt gattgccacg catcccatcg taggtgagct ccggcgcggc ggctgtcggc
     3781 ggggttttgg tgaagctgta agacggacgc tcaaaggacg cggaaatcgg agcccccgga
     3841 cagccacgcg gcgccccgga cgccacttga atcgccgtgc acgttacgca cgagcgacgt
     3901 gtcgacacgg tcgctgaaca cccagcgcgt ccacagtagc gggaccgatt tgtaccacct
     3961 gtcgatattg gacagaccac cgcccagcac gacgacatcg gggtcgacga tgttgatgac
     4021 atgcgcaagt gcacgtgcga gtcgctcttc gtagcgcgcg agcgtcgcct cgcaccgcgc
     4081 gtcgccgccg gcggcatgct cgacgactgc ctgcgtcgac gcttcgctgc cctcgtg
//
LOCUS       DASSVX010000054         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_418810, whole genome shotgun sequence.
ACCESSION   DASSVX010000054 DASSVX010000000
VERSION     DASSVX010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418810"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VFO53_02515"
     CDS             <1..1045
                     /locus_tag="VFO53_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875178.1"
                     /translation="GHGLDALIARHAIAVTHRHRALGDARAIWSFVEDLYRRQSHELV
                     DIAIRRILRMPSLPPQLPPDAIDTLPEAPGVYLFYGDNPLPLYVGKSINLRERVAAHF
                     AQDWRSETDLRLSREIRRIEHERTAGELGALLREAALVKATMPAHNRALRRKEEAGIA
                     RWHEAAPAFVAAGSLEPHGLAGAYGPFASRASMRAALRALASEHRLCPRRLGLERGSG
                     PCFARQLRRCDGVCAGEESARAHDARVAAALAPLAIPEWPVPGAACVREASADGERVD
                     VHVFRDWCWLGTARDDAELAALVGDRPCATFDLDVTRLLLRRYRARTLDLVPLPAACA
                     AMTCATQAVSRFA"
     gene            complement(955..2382)
                     /locus_tag="VFO53_02520"
     CDS             complement(955..2382)
                     /locus_tag="VFO53_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET8875179.1"
                     /translation="MIATDRIYRQWDDEIVPRLVDYIRIPAKSPHFDPAWQAHGHIER
                     VVELAFAWARAQPIAGLQVEVIRLPGRTPLLYFEIPGPGERTVLLYGHLDKQPEMTGW
                     RADAGPWTPVIENGRLYGRGGADDGYAIFAALAALGALHAQGAAHARCVGLIETCEES
                     GSYDLPAYLEALTPRIGNVDLVVGLDSGCGDYERLWMTTSLRGLAAGTLKVEVLQEGV
                     HSGDASGVVPSSFRIARKLLDRLEDATNGWILPSAFHAQIPPERVEQAGEAAAIMGDI
                     VLRKYPFVDGMRAMAEDGAEALLNRTWRPALSTIGADGFPAIADAGNVLRPSTSLMLS
                     LRLPPTIDGHDATVAMKSLFEADPPYGARVTFEPVQGATGWNAPPTQPWLAQAIDAAS
                     RAIYGKPSAAMGEGGTIPFMAMLGKQFPEAQFLITGVLGPHSNAHGPNEFLDIDYAKR
                     LTACVAHVIAAHAAGSGTRSSVRAR"
     gene            2413..>2746
                     /locus_tag="VFO53_02525"
     CDS             2413..>2746
                     /locus_tag="VFO53_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET8875180.1"
                     /translation="MPTIADAPPYAGLTPDCVLDALDSVGMRGDGRLLALNSYENRVY
                     QAWRDDAPPVVVKFYRPGRWSDAQIHEEHVLVAELAQADVPAVAPMVLGETTLHAFGG
                     YRFAVFARH"
BASE COUNT          414 a          966 c          934 g          432 t
ORIGIN      
        1 tggacacggg ctcgacgcgc tgattgcccg ccacgcaatc gcagtgacgc atcgtcatcg
       61 cgcgctcggc gatgcgcgcg cgatctggtc gttcgtcgag gacctgtacc gccggcaatc
      121 gcacgagctc gtcgacatcg cgatccggcg catcctgcgc atgccgagcc tgccgccgca
      181 gcttcctccc gatgccatcg acacgctgcc cgaagcgccc ggcgtctacc tgttctacgg
      241 cgacaatccg ctgccgctct acgtgggcaa gagcatcaac ctgcgcgagc gcgtcgccgc
      301 gcattttgcg caggactggc gcagcgaaac cgacttgcgg ctgtcgcgcg agatccggcg
      361 catcgagcac gagcgcaccg cgggcgagct cggcgcgctg ctgcgcgagg cggcgctcgt
      421 caaggcgacg atgcccgcgc acaaccgggc gctgcggcgc aaggaggaag cggggatcgc
      481 ccgctggcac gaagcggcac ctgccttcgt tgcagcaggc agcctcgagc cgcacgggct
      541 cgccggcgct tatggtccct tcgcgtcgcg tgcgtcgatg cgcgctgcgc tgcgcgcgct
      601 ggcctcggaa catcgcctct gcccgcgcag gctcggtctc gagcggggta gcggcccgtg
      661 ctttgcgcgg caattgcgcc gctgcgacgg cgtatgcgcg ggcgaagaat cggcgcgggc
      721 gcatgacgcg cgcgtggccg ccgcgctcgc gccgctggcc attcccgagt ggcccgtgcc
      781 aggcgccgcc tgcgtccgcg aagcgtcggc cgacggcgag cgcgtcgacg tccatgtctt
      841 tcgcgactgg tgctggctcg gcacggcgcg cgacgacgcc gagctcgccg cccttgtcgg
      901 agaccggccg tgcgcgacgt tcgatctcga cgtgacgcgc ctgttgcttc ggcgctatcg
      961 cgcccgcacg ctcgacctgg tgccactccc ggcggcgtgt gcggcgatga cgtgcgccac
     1021 gcaggccgtc agccgttttg cgtagtcgat gtcgagaaat tcgttgggtc cgtgcgcgtt
     1081 cgagtgcggg ccaagcacgc cggtgatcag gaattgcgcc tcgggaaatt gcttgccgag
     1141 catcgccatg aacgggatcg tgccgccctc gcccatcgcc gccgagggct tgccgtagat
     1201 cgcgcgggac gcagcgtcga tcgcctgcgc aagccatggc tgtgtcggtg gcgcattcca
     1261 gcccgtcgcg ccctgcacgg gctcgaacgt cacgcgtgcg ccgtacggcg gatcggcctc
     1321 gaacagcgat ttcatcgcga cggtggcgtc gtggccgtcg atcgtcgggg gcagccgcag
     1381 cgaaagcatc agcgacgtcg agggccgcag cacgtttccc gcgtcggcga tcgccggaaa
     1441 gccgtccgca ccgatcgtcg acagcgccgg ccgccacgtc cggttcagca acgcctcggc
     1501 gccgtcctcg gccatcgcgc gcatgccgtc gacgaacgga tacttgcgca acacgatgtc
     1561 gcccatgatc gccgccgcct cgccggcctg ctcgacgcgt tccggcggaa tctgcgcgtg
     1621 aaaggccgaa gggagaatcc atccgttcgt cgcatcctcg agccggtcga ggagcttgcg
     1681 cgcgatgcga aacgacgacg gcacgacgcc actcgcgtcg cccgaatgaa cgccttcctg
     1741 cagcacctcg accttcagtg ttccggccgc gagaccgcgc agcgacgtcg tcatccacag
     1801 ccgctcgtaa tcgccgcatc ccgagtcgag accgacgacg agatcgacgt tgccgatgcg
     1861 cggcgtgagc gcttcgaggt acgccgggag gtcgtaactg ccgctctcct cgcaggtctc
     1921 gatcagcccg acgcagcgcg catgcgcggc gccctgcgca tgcaatgcgc cgagcgctgc
     1981 cagcgccgcg aagatcgcat agccatcgtc ggcgccgccg cgcccgtaca gccggccgtt
     2041 ctcgatcacc ggcgtccacg gtccggcatc cgcacgccag ccggtcattt ccggctgctt
     2101 gtcgagatgt ccatagagga gcaccgtgcg ctcgccgggt ccgggaatct cgaagtaaag
     2161 aagcggcgtg cgaccgggca agcggatcac ttcgacctga aggcccgcga tcggctgcgc
     2221 acgcgcccag gcgaaggcga gttcgacgac acgctcgatg tggccgtgcg cctgccacgc
     2281 ggggtcgaag tgcggtgact tcgcgggaat gcgaatgtaa tcgacgaggc gaggaacgat
     2341 ctcgtcgtcc cattgccgat agatgcggtc ggtggcgatc atgtccgggg tatgcgcgag
     2401 aatgagacct gaatgccaac aatagcagac gcgccgcctt atgccgggct gacacccgat
     2461 tgcgtgctcg acgcgctcga cagcgtcggc atgcgcggtg acgggcgcct gcttgcgctc
     2521 aacagctacg agaatcgcgt ctaccaggcg tggcgcgacg atgcaccgcc cgtcgtcgtc
     2581 aagttctacc ggcccggccg ctggtccgat gcgcagattc acgaagagca tgtcctcgtc
     2641 gccgagcttg cgcaggccga cgtcccggcg gtcgcgccga tggtgctcgg cgaaaccacg
     2701 ctgcatgcgt tcggcggtta ccggttcgcc gtgttcgcgc gccacg
//
LOCUS       DASSVX010000055         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1235408, whole genome shotgun sequence.
ACCESSION   DASSVX010000055 DASSVX010000000
VERSION     DASSVX010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235408"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..482)
                     /locus_tag="VFO53_02530"
     CDS             complement(54..482)
                     /locus_tag="VFO53_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875181.1"
                     /translation="MSATQRVRHERRCTKPRCRHALALVLLAAAPLACAGPIEVYREG
                     PRFCPRDRAFGAPPISEAQAIERARRLLPDDYCGPSTFVSGCDVIPEHALGSWRIYFH
                     QYRVRDAQRDWGGLTHTYVVLDAVGNCDANIPGTEQGAPR"
     gene            complement(479..2722)
                     /locus_tag="VFO53_02535"
     CDS             complement(479..2722)
                     /locus_tag="VFO53_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET8875182.1"
                     /translation="MARLLRESWWLLVAACFVYLALTLFTYTKTDPGWSFSGTGAPIA
                     NRGGVAGAWLSDLLLYLFGLSAWWWVVGGIVLVVLGYRHVVHPERTSEHPIALAATGF
                     ALVLLASASLEAIRLWRLPATLPQKPGGALGEVIGDALARGIGFNGATLLLLALFAVG
                     TSLLFGVSWLRVMERIGAGVETLVARARARREAALDRRIGDLHAAEREQLVEHLREDV
                     AAHEPVVVVPALTAVPRSERATREKQRPLFTDMPDSPLPPLSLLEEAPPAAETVSAET
                     LEFTSRLIERKLADFGVAVKVLAAYPGPVITRYEVEPAVGVKGAQIVNLVKDLARALS
                     VVSIRVVETIPGKSCMALELPNARRQVVKLVELLASTTYNDAPGVLTLALGKDIAGKP
                     AITDLARTPHLLVAGTTGSGKSVAVNAMILSLLYKAEPRQVRLILIDPKMLELSVYDA
                     IPHLLAPVVTDMKLAPNALNWCVGEMERRYKLMSTLGVRNLAGYNQKIVDAKKAGKPL
                     THPFSLTPDAPEPLDEMPLIVVVVDELADLMMVTGKKIEELIARIAQKARAAGIHLIL
                     ATQRPSVDVITGLIKANIPSRIAFQVASKIDSRTILDQMGAEALLGQGDMLYLPPGTG
                     YPQRVHGAFVSDAEVHRVVEHLKAQGEPQYIEGILEGGTLSSDGDDAASVDGPDAESD
                     PMYDQAVQIVLKTRRPSISLVQRHLRIGYNRAARLIEQMERAGLVSAMQTNGNREVLV
                     PASKADA"
     gene            2927..3904
                     /gene="trxB"
                     /locus_tag="VFO53_02540"
     CDS             2927..3904
                     /gene="trxB"
                     /locus_tag="VFO53_02540"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET8875183.1"
                     /translation="MSVKHARLLILGSGPAGYTAAVYAARANLRPLLVTGLAQGGQLM
                     TTTDVDNWPADAEGVLGPELMARFERHAARFDTEIVFDHIHRADLSRRPFRLDGDNDA
                     YTCDALIIATGASARYLGLPSEEAHMGRGVSACATCDGFFYKGQDVAVVGGGNSAVEE
                     ALYLSNIARSVTLIHRRDRLRAEAILVDRLHAKTGTGGNVRMLWNHVLDEVLGDAKGV
                     TGVRVRDVQTAAAHEIAVHGVFVAIGHTPNTQIFEGQLEMSNGYIRVHGGAAGNATAT
                     SVPGVFAAGDVADQVYRQAVTSAGTGCMAALDADVFLETAGAAAETAMV"
     gene            3918..>4197
                     /locus_tag="VFO53_02545"
     CDS             3918..>4197
                     /locus_tag="VFO53_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875184.1"
                     /translation="MAKLTDLKALLDAATPAAPAVQPPVVPAPPARRTPARASDASAR
                     RIATGKRADGDIDLREAFSDVTRLPPRNRVAPARARPLPHARLRMEDER"
BASE COUNT          706 a         1438 c         1403 g          650 t
ORIGIN      
        1 gaccgccatg gcacgtgcgc cgtgcatcca gcccgacgcc gccttgccgc acttcatcgc
       61 ggtgctccct gctcggtccc ggggatgttt gcgtcgcaat tgccgacggc atcgagcacg
      121 acatacgtgt gcgtgaggcc gccccagtcg cgctgcgcgt cgcgcacgcg gtactggtgg
      181 aaatagatgc gccacgaccc gagcgcatgc tcggggatga cgtcgcagcc gctcacgaac
      241 gtgctcgggc cgcaatagtc gtcgggcagc agcctgcgtg cgcgctcgat cgcctgcgct
      301 tcgctgatcg gcggcgcgcc gaatgcacgg tcgcgcgggc agaaacgcgg tccctcgcga
      361 taaacctcga tgggacccgc gcaggcgagc ggcgctgccg caagcagcac gagcgcaagc
      421 gcatgcctgc atcgtggctt cgtgcagcga cgctcgtggc gcacgcgttg cgttgcgctc
      481 acgcgtcggc cttgctcgcg ggaaccagca cctcgcggtt cccattcgtc tgcatcgccg
      541 acacgaggcc ggcgcgctcc atctgctcga tcagccgtgc cgcccggtta tagccgatgc
      601 gcaaatgccg ctgcacgagc gagatcgacg ggcggcgcgt cttcagcacg atctggaccg
      661 cctggtcgta catcggatcc gattcggcat cggggccgtc gacgcttgcc gcgtcgtcgc
      721 cgtcggaaga cagcgtaccg ccttcgagga tgccttcgat gtattgcggt tcgccctgtg
      781 ccttcagatg ctcgacgacg cgatgcactt ccgcgtcgga cacgaacgca ccatgcacgc
      841 gctgcgggta accggtgccc ggaggcaggt agagcatgtc gccctggccg agcaacgcct
      901 cggcgcccat ctggtcgagg atcgtgcgcg agtcgatctt gctcgcgacc tggaacgcga
      961 tgcgcgatgg aatgtttgcc ttgatgagac cggtgatgac gtcgaccgac ggccgctgcg
     1021 tcgcgaggat caggtgaatt cccgcggcgc gcgccttctg tgcgatgcgc gcaatgagct
     1081 cctcgatctt cttcccggtc accatcatga ggtcggcgag ctcgtcgacg acgacgacga
     1141 tcagcggcat ctcgtcgagc ggctccggcg catcgggcgt gagcgagaat ggatgcgtga
     1201 gcggcttgcc ggcctttttc gcgtcgacga tcttctggtt gtagccggcg agattgcgca
     1261 cgccgagcgt cgacatcagc ttgtagcgcc gctccatttc gccgacgcac cagttgagcg
     1321 cattgggcgc gagcttcatg tcggtgacga cgggtgccag cagatgcgga atcgcgtcgt
     1381 acaccgaaag ctccagcatc ttcggatcga tcaggatcag ccgcacctgc cgcggctccg
     1441 ccttgtagag cagcgacagg atcatcgcgt tgaccgcaac cgacttgccc gaccccgtcg
     1501 ttcccgcgac gagcagatgc ggcgtgcgcg cgagatcggt gatcgcaggc ttgcccgcga
     1561 tgtccttgcc gagtgcgagc gtcagcacgc ccggcgcatc gttgtacgtc gtcgacgcga
     1621 ggagctcgac gagcttcacg acctggcggc gcgcattggg caattcgagc gccatgcacg
     1681 acttgcccgg gatcgtctcg acgacgcgga tcgagacgac cgacaacgcg cgcgcgagat
     1741 ccttgacgag gttgacgatc tgcgcaccct tgacgccgac cgccggctcg acttcgtagc
     1801 gcgtgatgac gggccccggg taggcagcga gcaccttcac cgcgacaccg aagtcggcga
     1861 gcttgcgctc gatcagccgc gacgtgaact cgagcgtttc ggcactgacc gtttcggccg
     1921 cgggcggcgc ctcctcgagc agcgacagtg gcggcagcgg cgaatcgggc atgtcggtga
     1981 aaagcggccg ctgcttctcg cgcgtggcac gctccgagcg cgggacggcg gtgagcgcgg
     2041 gcaccacgac gaccggttcg tgcgcggcga cgtcctcgcg cagatgctcg accaactgct
     2101 cgcgctcggc ggcgtgcagg tcgccgatgc gccgatccag cgccgcctcg cggcgcgcgc
     2161 gcgcacgggc gacgagcgtc tccaccccgg cgccgatacg ctccatcacg cgcagccatg
     2221 acacgccgaa cagcagcgac gtgccgacgg cgaacagcgc gagcagcaaa agcgttgcgc
     2281 cattgaagcc gatgcctcgc gccagcgcgt cgccgatcac ttcgccgagc gctcctcccg
     2341 gtttctgcgg cagcgtggcc ggcagccgcc acaagcggat tgcctcgagc gatgcgctcg
     2401 ccagaagcac gagcgcaaaa ccggtcgccg ccagcgcgat cggatgctcg ctcgtccgct
     2461 cgggatgcac gacgtgccga tagccgagca cgaccaacac gatgccaccg acgacccacc
     2521 accaggccga gaggccgaac aggtagagca acagatcgga gagccatgca ccggccacgc
     2581 cgccgcggtt ggcaatcggc gcaccggtgc cggaaaacga ccagccgggg tcggtcttcg
     2641 tgtacgtgaa gagggtcagc gcgaggtaga cgaagcacgc ggcgacgagc agccaccacg
     2701 attcgcgcag cagccgcgcc atgcgcggcg acagttcgcg cggcggggct tgcgaacgtc
     2761 ggcgcgagtt gccgtttttc ttggaaaaca tgagactagc gcgagaacct catgcagccc
     2821 attatacggg cgcgagaagg cgattcgcaa cggcaggctt gcttgcgccg ccggaccgcg
     2881 tccccatctg cattaaactt caaggtcttg cgtcaacgag aatgccatgt ccgtcaagca
     2941 cgcccgcctg ttgatcctgg gatccggccc cgccggctat acggcggcag tatacgctgc
     3001 gcgtgccaat cttcgcccgc tcctcgtgac cggactcgca caaggcggac agctgatgac
     3061 gaccaccgac gtcgacaact ggcccgccga tgccgagggg gttctcggac ccgagctgat
     3121 ggcgcgtttc gagaggcacg ccgcacggtt cgacaccgaa atcgtcttcg accacatcca
     3181 ccgtgccgac ctgtcccgcc gaccgtttcg cctggatggc gacaacgatg cgtacacgtg
     3241 cgacgcgctg atcatcgcga ccggcgcttc cgcccggtat ctcggactgc cgtcggaaga
     3301 ggcgcacatg ggccgcggcg tttccgcttg cgcgacctgc gacggattct tctacaaggg
     3361 ccaggacgtg gccgtcgtcg gcggcggcaa ttcggcggtc gaggaagccc tctacctgtc
     3421 gaacatcgcg cgcagcgtca cgctgatcca tcggcgcgac cgcctgcgcg ccgaggccat
     3481 cctagtcgac cgcctgcatg cgaaaaccgg cacgggcggc aatgtccgca tgctttggaa
     3541 tcacgtcctc gatgaagtgc tgggcgatgc aaagggtgtc accggggtgc gagtacgcga
     3601 cgtgcaaacg gcggcggcgc acgaaatcgc cgtgcacggc gtgttcgtcg cgatcggcca
     3661 cacgccgaac acgcagatct tcgaaggcca gctcgagatg tcgaacggct acattcgcgt
     3721 gcacggaggc gctgcgggca atgcaacggc gacgagcgtc ccgggcgtct tcgccgcggg
     3781 cgacgtggcc gatcaggtgt accgccaggc ggtgacgtcc gccggaaccg gatgcatggc
     3841 cgcgctcgac gccgacgtgt tcctcgaaac cgcgggcgcc gcggccgaga ccgcgatggt
     3901 gtgattcacg ctgcgacatg gccaagctga ccgatctcaa ggcgctgctc gacgccgcga
     3961 cgcccgccgc gccggcagtg caaccgccgg tcgtgccggc gccaccggcg agaaggactc
     4021 ctgcgcgcgc atccgatgcg tcggcgcgcc gcattgcaac cggcaagcgt gccgacggcg
     4081 acatcgacct gcgcgaggcg ttctcggacg tcacgcgcct gccgccacga aaccgcgttg
     4141 caccggcgcg cgcgcgtccg ttgccgcatg cgcggctgcg catggaggac gagcgcg
//
LOCUS       DASSVX010000056         3772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_685399, whole genome shotgun sequence.
ACCESSION   DASSVX010000056 DASSVX010000000
VERSION     DASSVX010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685399"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFO53_02550"
     CDS             <1..577
                     /locus_tag="VFO53_02550"
                     /inference="COORDINATES: protein motif:HMM:NF025397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HET8875185.1"
                     /translation="AAQHLREPVARRAAIGDAARMARRTREMIAGAGIDCELVTGAGT
                     GTWQHERDSGAYDELQPGSYVFMDADYGRNIAAPDELRFEQSLYVLASVVSVPAPERA
                     IVDAGLKAFSFDSGLPLARGRAGVEYVKASDEHGVLAVAPDAPPLRSDERVFLIPGHC
                     DPTVNLYDYLVAVRDGVVEGVWPIEARGALG"
     gene            complement(589..1509)
                     /locus_tag="VFO53_02555"
     CDS             complement(589..1509)
                     /locus_tag="VFO53_02555"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8875186.1"
                     /translation="MPLDPALGELIRAKLAHASAPQWAMPIAEVRRAFSALWTPAMTG
                     APPTVARIEDIAIPAAGAGVRARVYAPSGERRPVMLYFHGGGYVKGGIDDSDGFCRSL
                     ARAADHIVLSVDYRLAPEHPFPAALDDAYAATSWTCAHLADLGGAPGPVVVCGESAGG
                     NLAAVTCLRARDDPHIAIRFQVLLQPVVDFTLSFASMALPATECLVPRDDLAWYYRTY
                     YGDERDRRDARVSPLYADDLSRLPPALIIAAEFDTLRDEAAAYAERLVASGVPARYHC
                     YPGMVHGFLQMGGVVPQAGDALREIAQAIA"
     gene            complement(1535..2206)
                     /locus_tag="VFO53_02560"
     CDS             complement(1535..2206)
                     /locus_tag="VFO53_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013556050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8875187.1"
                     /translation="MADQIVSRHAAPPDLAADHLIVGAASLRAGIEWAESRLGVRAQA
                     GGQHVTMGTHNALLGLGPRLFLEIIAIDPDATVPTRPRWFDLDEPRMRARLIESPALI
                     HWVARSRDLEADAAHCSVDLGTVTPMRRGDYAWRLTIPADGHLPERGLVPTLIQWSDH
                     RHPADALPDAGLRLVALAGEHPDPAPVRRALIALGVGDTLKVTYGRTPRLAAMLRTPR
                     GTVTL"
     gene            complement(2214..2615)
                     /locus_tag="VFO53_02565"
     CDS             complement(2214..2615)
                     /locus_tag="VFO53_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8875188.1"
                     /translation="MPATAMNHFTILTDDVRRTVDFYCALLGLTEGARPPLQFPGAWL
                     YAGGNAILHVVGGRSADELKAGVIDHMAFSATDLADTLALLTARNIAHTCRRQAGSGT
                     WQVFFHDPNGARVELDFAADEPQHAAARSPA"
     gene            complement(2651..3349)
                     /locus_tag="VFO53_02570"
     CDS             complement(2651..3349)
                     /locus_tag="VFO53_02570"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET8875189.1"
                     /translation="MPATAFQRVAADAGEPAVRKRDPMQTRDRVLRAGIVEFCRHGYI
                     GARIDKIAKRAGCNMRMIYHYFGSKEKLYVAVLERVFGDLRLEESRLHLEHLDPVTGV
                     LRLVEFTMDHLGRHPEFVALLGNENLLRGKYLRKSHAVPRASAPLLEAIRGLLERGLR
                     EHVYARDVDPLQLYVSILSLCYVHVSNRYTLSITFNRDITDPGWLDARRRHVQQMVLA
                     YLCGADGVRESARA"
     gene            complement(3364..>3772)
                     /locus_tag="VFO53_02575"
     CDS             complement(3364..>3772)
                     /locus_tag="VFO53_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8875190.1"
                     /translation="FLMGRGIDHDVPRGIAPVHRLVHAGANASLSTNNVLNPFTPYGD
                     CSLLRMANLYANASQLGQQGELALCHAMITNRAARILGVTDHRVAVGAAADLVVLDCA
                     SGAQAVAQIATPLFVFKRGQMTVSRAPAVLHRP"
BASE COUNT          601 a         1251 c         1351 g          569 t
ORIGIN      
        1 cgccgcgcaa cacctgcgcg aaccggtcgc tcgccgtgcg gcgatcggcg acgccgcgcg
       61 catggcgcgg cgcacgcgcg agatgatcgc cggcgccggc atcgactgtg agctcgtcac
      121 cggtgcgggt accggcacat ggcagcacga gcgcgactcg ggcgcctacg acgagctgca
      181 accgggatcg tacgtgttca tggatgccga ttacggccgc aacatcgcgg cacccgacga
      241 gctccggttc gagcagagcc tctacgtgct cgcaagcgtc gtcagcgtgc cggcacccga
      301 acgcgccatt gtcgacgccg ggctcaaggc cttttcgttc gactcgggtt tgccgctcgc
      361 gcgtgggcgt gcgggcgtcg agtacgtgaa ggcgtcggac gagcacggtg tgctcgcagt
      421 ggcgccggat gcgccgccac ttcgcagcga cgaacgcgtg ttcctgattc cgggccactg
      481 cgacccgacc gtgaacctct acgattacct ggtcgccgtg cgagacggcg tcgtcgaagg
      541 cgtgtggccg atcgaagcgc gcggcgcgct gggctgaccg gcagcgcgtc aggcgatcgc
      601 ctgcgcgatc tcgcgcagcg catccccggc ctgcggaacc acgccgccca tttgcaggaa
      661 tccgtgcacc atgcccggat agcagtggta ccgggcaggc acccccgacg ccaccaatcg
      721 ttcggcgtag gccgccgcct cgtcgcgcaa cgtatcgaac tccgcggcga tgatcagcgc
      781 cggcggcagc cgtgaaaggt catcggcgta gagcggagag acgcgcgcgt cgcgccgatc
      841 gcgttcgtcg ccgtaataag tgcggtagta ccaggcgaga tcgtccctcg gcacgaggca
      901 ttcggttgca ggcagcgcca tcgacgcaaa cgaaagcgtg aaatcgacca ccggctgcag
      961 cagcacctgg aagcggatgg cgatgtgcgg atcgtccctc gcgcgaaggc aggtgaccgc
     1021 agcgagatta ccgcccgcgc tctcgccgca gaccaccacc gggcccggtg cgcctccgag
     1081 atccgcaaga tgcgcgcagg tccatgacgt tgcggcataa gcatcgtcga gcgccgcggg
     1141 aaaggggtgc tcgggtgcca ggcggtagtc gaccgacagc acgatgtgat cggcggcgcg
     1201 ggcgaggctg cggcaaaagc cgtcgctgtc atcgatgccg cccttcacgt agccgccgcc
     1261 gtggaagtac aacatgaccg ggcgccgttc gcccgacggt gcatagacgc gggctcgaac
     1321 gccggcgccg gccgcgggaa tcgcgatgtc ctcgatgcgc gcgacggtgg gcggcgctcc
     1381 cgtcatcgca ggcgtccaga gcgcgctgaa ggcgcggcga acctccgcga tcggcatcgc
     1441 ccattgcggt gccgatgcgt gcgcaagctt ggccctgatc agctcgccga gcgcaggatc
     1501 gagtggcatc gctatcctcg cttggttcga tgggtcaaag cgtgaccgtg ccgcgcggcg
     1561 ttcgcaacat cgcggcgagg cgcggcgtgc gcccgtaggt caccttcagc gtgtcgccga
     1621 cgcccagtgc gatcaatgcc ctgcgcaccg gcgcggggtc ggggtgctca ccggcgagtg
     1681 cgacgagtcg caacccggca tcgggtagcg cgtcggccgg gtgccggtga tcggaccatt
     1741 gaatcagcgt cggcaccagg ccgcgctcgg gcaggtgtcc gtcggcgggg atcgtcaagc
     1801 gccatgcgta gtcgccccgg cgcatcggcg tgacggtgcc gaggtcgaca ctgcaatgcg
     1861 ccgcatcggc ttcgagatcg cgcgagcgcg ctacccagtg aatcaacgcg ggcgattcga
     1921 tcagccgtgc gcgcatccgc ggctcgtcga ggtcgaacca gcgcggtcgc gtgggcacgg
     1981 ttgcgtctgg atcgatggcg atgatctcga gaaagaggcg gggaccgagc ccgagcagcg
     2041 cattgtgcgt gcccatcgtc acgtgctgcc cacctgcctg ggcgcgaacg cccaggcggc
     2101 tctcggccca ttcgatgccg gctcggagcg acgccgcgcc gacgatcaga tgatcggcgg
     2161 cgaggtccgg tggcgcggcg tgccgcgaaa cgatctgatc ggccatccgc cactcaggcc
     2221 ggcgacctgg ccgccgcgtg ctgcggctcg tcggccgcga agtcgagctc gacccgcgcg
     2281 ccgttcggat cgtggaagaa cacctgccac gttcccgagc ccgcctgccg gcggcaggtg
     2341 tgcgcaatgt tgcgcgcggt gaggagcgcc agcgtgtcgg cgaggtcggt cgccgagaac
     2401 gccatgtgat cgatgacgcc ggccttgagc tcatccgccg aacggccgcc aacgacatgc
     2461 aggatcgcgt tgcctcctgc atagagccag gcgccgggaa actgcagcgg cggccgcgcc
     2521 ccttcggtga ggccgagaag cgcgcaatag aagtcgacgg tccggcgcac gtcgtccgtc
     2581 aggatggtga aatgattcat cgcggtggcg ggcatggcgg attcctcgcg cgaggtgagg
     2641 ctcgattatc tcaggcgcgg gcgctttcgc gcacgccgtc ggcaccgcac aggtacgcga
     2701 gcaccatctg ctgcacgtgc cggcggcgtg catcgagcca gccggggtcg gtgatgtcgc
     2761 gattgaacgt gatcgacagc gtgtagcggt tggacacgtg cacgtagcat agcgacagga
     2821 tgctgacgta gagctgcagc gggtcgacgt cgcgtgcata cacgtgctcg cgcaggcccc
     2881 gctccaggag tccgcggatg gcctcgagca gcggcgccga cgcacgcgga accgcgtgcg
     2941 atttgcgcag gtacttgccg cgcagcaggt tctcgttgcc gagcagcgcg acgaactcgg
     3001 ggtggcgccc gagatggtcc atcgtgaact cgacgagtcg cagcacgccg gtgacgggat
     3061 cgaggtgctc gaggtgcagc cggctttcct cgagccgcag gtcgccgaac acgcgctcga
     3121 gcaccgcgac gtagagcttc tccttgctgc cgaaatagtg gtagatcatg cgcatgttgc
     3181 agcccgcacg cttcgcgatc ttgtcgatgc gcgcgccgat gtagccgtgc cggcagaatt
     3241 cgacgatgcc ggcgcgcagc acgcgatcgc gcgtttgcat cggatcgcgc ttgcggaccg
     3301 ccggctcgcc cgcgtccgcg gcgacgcgct ggaaggcagt cgcgggcatc ggccgttcgc
     3361 gtcctatggc cggtgcagca cggcaggggc ccggctgacg gtcatttgcc cgcgcttgaa
     3421 cacgaagagc ggcgtcgcga tttgcgcgac cgcctgtgca ccactcgcgc agtcgaggac
     3481 cacaaggtcg gcggccgcac cgacggccac gcgatgatcg gtcacgccga ggattcgcgc
     3541 ggcgcggttg gtgatcatcg cgtggcacag cgcgagctcg ccttgctggc cgagctggct
     3601 cgcgttggca tacaggttcg ccatgcgcag tagcgagcaa tcgccgtagg gcgtgaacgg
     3661 gttcagcacg ttgttggtcg acagcgacgc gttcgcgccg gcgtgcacga ggcgatgcac
     3721 cggcgcgatg ccgcgcggca cgtcgtgatc gatgccgcgg cccatcagga ag
//
LOCUS       DASSVX010000057        16271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_702088, whole genome shotgun sequence.
ACCESSION   DASSVX010000057 DASSVX010000000
VERSION     DASSVX010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702088"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1263)
                     /gene="tuf"
                     /locus_tag="VFO53_02580"
     CDS             complement(73..1263)
                     /gene="tuf"
                     /locus_tag="VFO53_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET8875191.1"
                     /translation="MAKSKFERTKPHVNVGTIGHVDHGKTTLTAAITSVLSKKFGGEA
                     KAYDQIDNAPEEKARGITINTAHVEYETSKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVYLNKADMVDDAELLELVEMEVRELLSK
                     YQFPGDDTPIVIGSAKLALEGDKGEMGEGSIMKLADALDSYIPQPQRLVDGPFLMPVE
                     DVFSISGRGTVVTGRVERGVVKVGENLEIVGLKPTLQTVCTGVEMFRKLLDQGQAGDN
                     VGVLLRGTKREEVERGQVLAKPGSITPHTKFSCEVYVLSKEEGGRHTPFFNGYRPQFY
                     FRTTDVTGSVELPSGTEMVMPGDNIAMTVTLIAPIAMEEGLRFAIREGGRTVGAGVVS
                     KVIE"
     gene            complement(1379..1453)
                     /locus_tag="VFO53_02585"
     tRNA            complement(1379..1453)
                     /locus_tag="VFO53_02585"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            complement(1496..1569)
                     /locus_tag="VFO53_02590"
     tRNA            complement(1496..1569)
                     /locus_tag="VFO53_02590"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1535..1537),aa:Gly,seq:tcc)
     gene            complement(1680..1761)
                     /locus_tag="VFO53_02595"
     tRNA            complement(1680..1761)
                     /locus_tag="VFO53_02595"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1725..1727),aa:Tyr,seq:gta)
     gene            1927..3540
                     /locus_tag="VFO53_02600"
     CDS             1927..3540
                     /locus_tag="VFO53_02600"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HET8875192.1"
                     /translation="MPPATASFDYVVIGAGSAGCVLANRLSEDGRYTVLLLEAGPRDT
                     YPWIHIPIGYAKTMFHPVVNWGFYTEPEPAMNGRKVYWPRGRTLGGSSAINGLISIRG
                     QREDYDAWAAAGNTGWSYADVLPWFKRLEHNVRGASEWHGADGPLWASDIGAAHPLIE
                     AMIAGAGELGIPANDDFNGATQEGAGYYQLTTRNGRRCSTAVAYLKPARARRNLHVET
                     NAHATRIVLENRRATGVVYRQRGTERTVGANREVILAAGALQSPQLLQLSGIGPPSLL
                     QSLGIPVQHPLEGVGENLQDHLQARAIFRCTQPITTNDALRTLGGKLAMGIDYVFRRR
                     GPMAIGINQGGIFARAMPGATRPDVQFHFATLSSDMAGSPVHTFPAFTMSVCQLRPTS
                     RGTVRIKSTNPLEPPAMQPRYLSTPEDRATIVAGIRLARALAATHALRPYVENEYRPG
                     PQVQSDDDLLEFVKNTGGTIFHPSGTTRMGPASDPGAVVDPELRVHGVERLRVIDCGI
                     MPTLVSGNTNVPVVMIAEKTAAAILAGDR"
     gene            3625..3864
                     /locus_tag="VFO53_02605"
     CDS             3625..3864
                     /locus_tag="VFO53_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HET8875193.1"
                     /translation="MATTITSKGQVTIPKRVREALRLAPGDSVDFAVNPTGQVVLQKV
                     APRSTAKRDRFESARGKAQVKWRTNDLMALLRGDD"
     gene            3874..4290
                     /locus_tag="VFO53_02610"
     CDS             3874..4290
                     /locus_tag="VFO53_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010636976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET8875194.1"
                     /translation="MLLVDTNVLIDVLENDADWADWSVRQLRAQAQVHELTINPVIYA
                     ELSLLFESADALDDAIAKMNLQFQELPRAALFLAGRAFMKYRRAGGNRSNVLADYFIG
                     AHAAVAGCGILTRDAGRYAGYFPRVPLVTPHTASAR"
     gene            4329..5639
                     /locus_tag="VFO53_02615"
     CDS             4329..5639
                     /locus_tag="VFO53_02615"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HET8875195.1"
                     /translation="MACCHADFAALEGSDRGFTLGMPTFTFGPGVLAEAGDNAAELGL
                     KRVALFTDRRLASGEHVAKVKASLAAANVDCVVYDDVVIEPTDKSLQEAARFARESNV
                     DGYVSVGGGSVMDTCKAANLYASQPAEFMTYVNAPIGAGQRVPGPVKPHIACPTTSGT
                     GSETTGISIFTLTSMNAKTGIISRRLIPTIALVDPDVTRSLPANVVASTGFDCMSHAL
                     ESITARLYPRRLNPAQGIRRPVSQGANPFSDALATQALTSVGQYLVRAVDDASDTEAR
                     TEMMYAAMLAGIAFNAAGCHLPHGLSYAVSGLIKDWHVDGYPEGKSLIPHGMSVVLNN
                     PSVWRHTAFAHPERHLRCARALGGATNGATHTDAGEVLAGRIIELMRAAGMPNGLTAL
                     GFGEAQIDALATGAEPQYRVIKNAPIDVDRDVIKRLFRAALRYW"
     gene            complement(5602..6987)
                     /gene="phoR"
                     /locus_tag="VFO53_02620"
     CDS             complement(5602..6987)
                     /gene="phoR"
                     /locus_tag="VFO53_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HET8875196.1"
                     /translation="MSSFAWRALRAPAAVALLTVLCAAAGGAALALAVLALGASAIIA
                     FHLFHLHRLDVWARAAPDTDVPEGRGAWAAPLGSIHRRVRARNAYERELRQVIERFRQ
                     AATAIPDGIVVLDAANRIDWANARALAQLGLDLAKDRGQPIANLVRQPEFRRYVASGD
                     YGAPVVVESDREAACTLALQLVPFAHDQKLLIARDITGMEALARVRRDFIASVSHELK
                     TPLTVISGFIETLQDVDVGMRERARFLQLMQEQATSMRRLVADLLTLSALETEQGPVH
                     EERFAILPLLTGLSSDAGALSNRAHRIGVDAADAATVIGSREEIASAFGNLVSNAVRY
                     TPPGGTITLSWRIDVDGSGVFAVCDTGIGIAAEHIPRLTERFYRVDRSRSRATGGTGL
                     GLAIVKHVLLRHQGDLAIASERGNGSTFTVRLPAQRVERAPPAGRASAGSGSTETEVP
                     TSIEVPRETGA"
     gene            complement(6984..7712)
                     /gene="phoB"
                     /locus_tag="VFO53_02625"
     CDS             complement(6984..7712)
                     /gene="phoB"
                     /locus_tag="VFO53_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HET8875197.1"
                     /translation="MTPSILVVEDEPAILELLKFSLQDAGYEVLEALDAAAAQRALAQ
                     RLPDLVLLDWMLPGQSGLALARQLRADRRTRELPLIMVTARTEEADKIAGLEAWVDDY
                     VTKPFSPRELKARIKAVLRRRAPEAAQDTLRSGKLTLDGVAHRVSVAGTDIPLGPTEF
                     RMLRFLMARPDRVLTRTQLLDGVWGDHVYIEERTVDVHIRRLRLALEASGAASAIETV
                     RGSGYRFVSHPAADASPEADAGRR"
     gene            complement(7732..8439)
                     /gene="phoU"
                     /locus_tag="VFO53_02630"
     CDS             complement(7732..8439)
                     /gene="phoU"
                     /locus_tag="VFO53_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET8875198.1"
                     /translation="MTEHTNKQFDAEMEAIRSGVLSMGGTVERQLRRAVDALENDDDG
                     ARGGAVAADEAAINQLQMAIDQQCAQIIARRQPAAVDLRIVLTVSKIVNDLERSGDEA
                     KKIAYRAAETRGSERLARVRHYDVLRALDRATTMLREALDAFARLDAVAAAEIVARDA
                     EIDAAFASILRQLVSYMMEDPRTITPALEIVFVAKSIERIGDHAKNIAEAVVQVVMGK
                     DVRHASPDEIRAEVAGR"
     gene            complement(8448..9191)
                     /gene="pstB"
                     /locus_tag="VFO53_02635"
     CDS             complement(8448..9191)
                     /gene="pstB"
                     /locus_tag="VFO53_02635"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HET8875199.1"
                     /translation="MSIRNLDFFYGGFQALAHVDLDIAEREVTAFIGPSGCGKSTLLR
                     TLNRMYSLYPGQRATGEVRFNGVNILDASTDVNLLRAKVGMVFQKPTPFPMSIHENVA
                     FGVRLYERLSARAMDERVEWALTKAAMWNEVKDKLPQSGMALSGGQQQRLCIARAIAV
                     LPEVLLLDEPTSALDPISTARIEELISELKADYTIAMVTHNMQQAARVSDRTAYMYLG
                     ELVEFGPTDQVFLKPARRETEDYITGRFG"
     gene            complement(9275..10117)
                     /gene="pstA"
                     /locus_tag="VFO53_02640"
     CDS             complement(9275..10117)
                     /gene="pstA"
                     /locus_tag="VFO53_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET8875200.1"
                     /translation="MTRRYRRRLFVSRFNLAMSLATMALGMLFLLWILATLFANGLAA
                     LSPSLFTQMTPPPGAAGGLRNAIFGSVLIVATATLVSTPIGMLAGIYLAEYGRSGWLA
                     EATRFVNDMLLSAPSIVLGLFVYAVYVLQIGHFSGWAGSLALALIAIPVVVRTTENTL
                     RLVPGSLREAAVALGAPRWKVIVKVTLKAARGGVITGILLAVARMSGETAPLLFTALN
                     NQFWSTDMNAPIANLPVVIFQFAMSPFDDWKTLAWAGALLITLAVLTLNIVARAVFRP
                     SALR"
     gene            complement(10242..11186)
                     /gene="pstC"
                     /locus_tag="VFO53_02645"
     CDS             complement(10242..11186)
                     /gene="pstC"
                     /locus_tag="VFO53_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET8875201.1"
                     /translation="MRRAAAGRRQDALFAHATRAFAALVLMLLVAIVIALGYAAWPAL
                     SKFGPRFFISNAWNPVTSEFGALAPIYGTIVTSAIALLIGVPVSFGIALFLTEMCPPS
                     LKRPLGTAVELLAAIPSIIYGMWGLFVFAPFFADHVEPLLTGTLGNAWVIGPLFQGAP
                     TGLGVLTAGIILAVMVIPFIAAVMRDVFDIVPPVLKESAYAMGCTTWEVVRSVVLPYT
                     RAGVVGGIMLGLGRALGETMAVTFVIGNAYRIRASLLEPGNSIASALANEFNEAADPV
                     HRGALIALGLVLFVLTLIVLAASRLLIARLTRGEGAPA"
     gene            complement(11251..12306)
                     /gene="pstS"
                     /locus_tag="VFO53_02650"
     CDS             complement(11251..12306)
                     /gene="pstS"
                     /locus_tag="VFO53_02650"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET8875202.1"
                     /translation="MRSMKAGLTAIAAALAVPLAPAVAADITGAGATFPFPVYAKWAA
                     AYKTTTGIGMNYQSIGSGGGIAQIKAKTIDFGASDMPLRPEELKRAGLVQFPAIIGGV
                     VPVMNVDGVAPGALKLTGPVLADIYLGRVKRWNDKAIAELNAGIRLPDEAIAVVHRSD
                     GSGTTFLWTEYLSKVSIEWKTKVGAGTAVAWPEGVGGKGNEGVAAYVQRIKGALGYVE
                     YAYAKRNGMAHALVRNRDGQYPAPDDSTFQAAAANADWKNAPGFYRILTDEPGASAWP
                     ITGASFILLHATQQKPQNAVQVLKFFDWSFRNGQKLAGELDYVALPPNLVELVESSWK
                     ATINDASGKPIWPASGE"
     gene            complement(12439..13179)
                     /locus_tag="VFO53_02655"
     CDS             complement(12439..13179)
                     /locus_tag="VFO53_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875203.1"
                     /translation="MSGGVLPPVDRAAVLEARNVHAFYGKSHILHGVSLRLDEGEIVT
                     VLGRNGAGKTTTMRSLMGLTAVREGSVHMLGQDTTRWPAYRIARLGVGYVPEGRKIFG
                     NLTVLENLTVPEARPGAWTLDRVFKLFPRLGERKTQLGRQLSGGEQEMLSIARPLLLN
                     PRVMLLDEPSQGLAPLIVQEVMQVVRAMRDEGLSVLLVEQNVPLSLGIADRAYILDDG
                     KVVYSGTAAELAANHDLVEKLAGATAQH"
     gene            complement(13176..14012)
                     /locus_tag="VFO53_02660"
     CDS             complement(13176..14012)
                     /locus_tag="VFO53_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875204.1"
                     /translation="MSAAEPPQGANCAPSGGSAAAKPQAWGDHTSLLRLANVSRHFGK
                     LQAVQDVSFSMDEGELRAVIGPNGAGKTTFFNLITGFVPPTSGDIWFDDENIRDRTVV
                     HRVRKGIVRTFQITEVFPDLTIYENVRVGAETAAGLNGRPWLPRAMRADIARQVDELL
                     DVVGLETKADKVVGELAHGDQRVVEIALALAMNPRLLLLDEPTAGMGDTETDHMVDLV
                     RRLHAQRKLSILFIEHDMSIVFGIAQRITVLDQGRVLAEGSADEISHNDAVRAAYLGT
                     DA"
     gene            complement(14056..15090)
                     /locus_tag="VFO53_02665"
     CDS             complement(14056..15090)
                     /locus_tag="VFO53_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8875205.1"
                     /translation="MMRRHAPEIVLWIALLTVPLWLGYVGGYAALGSKILIYGIAALA
                     LNLVLGFTGGLSFGHAAYFGLGAYGAGMTLKYLVPSTPLAILVGTVVGGLAATLLGPL
                     VMRRRGIYFAMITIAIGQLFYFVVVRWNNVTGGEDGLAGFRRQPLHLGSFVYDAQSES
                     KYYYLVLLCFAVAVVIMRVLLASPLGHTWVAIRENRRRAQFLGVRTDFYVWAAFAISG
                     TLTALAGTLNAMLFNFASPQDLHWILSGNFVIMIVLGGMRKFWGPLVGVTIFVVAQDY
                     LSSLTDNWMTAIGLLFVLIVLLFPKGILGLVRRRGRELAVAVTGPRAAIERSTATIEP
                     PPAPLKQEMK"
     gene            complement(15087..15956)
                     /locus_tag="VFO53_02670"
     CDS             complement(15087..15956)
                     /locus_tag="VFO53_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8875206.1"
                     /translation="MQLLLFNAFNGLIQGVFYALMALGLALILSLNSVINMAHGGFLV
                     IGAYLAYSLAPYLGFWGALVVGPVGAAIIGLGVERVLIRPLYKRPDPLYTLLLTFGLA
                     LIIEDLTRTIWGAQGLPFQVPSALNSPISSVLFFITGYRLLVVAITMAVMLGLYLLLR
                     YTRLGIRIRAGTFDLETVSALGVNIYLLRALNFALGCLLAGLAGVLAAGILGLTPPMG
                     SSLIMPSFVAIIVGGVGSLVGSILGGLLIGVAAGITTTYYPAASEAVIYLIMLIVLVI
                     RPRGLLGQEGLFE"
BASE COUNT         2798 a         5606 c         5230 g         2637 t
ORIGIN      
        1 gcgggcggag cccgacgatt cgatgcgtaa actgctgcga aaaacgagcg tgcgagcaat
       61 cagccgatcg cgctactcga tcaccttgga tacgacaccg gctccgaccg tacggccacc
      121 ctcgcggatg gcgaagcgca gtccctcttc catcgcgatc ggcgcaatca gcgtcaccgt
      181 catcgcaatg ttgtcccccg gcatcaccat ctccgtcccc gacggcagct ccacgctccc
      241 cgtcacgtcg gtcgtgcgga aatagaactg cggccggtag ccattgaaaa acggcgtgtg
      301 ccgacccccc tcctccttgg acagcacgta cacctcgcag ctgaacttcg tgtgcggcgt
      361 gatcgacccc ggcttcgcca gcacctgacc ccgctccact tcctcccgct tcgtgccgcg
      421 cagcagcacc cccacgttgt cacccgcctg accctggtcc agcagcttgc ggaacatctc
      481 cacccccgtg cacaccgtct gcaacgtcgg cttcagcccc acgatttcca ggttctcgcc
      541 caccttcacc accccgcgct ccacccgacc cgtcaccacc gtccctcgcc ccgagatcga
      601 gaacacgtcc tccaccggca tcaggaacgg accgtccacc agccgctgcg gctgcggtat
      661 ataactgtcc agcgcatcgg cgagcttcat gatcgagccc tcgcccatct cgcccttgtc
      721 cccctccagc gccagcttcg ccgacccgat cacgatcggc gtgtcgtccc cgggaaactg
      781 gtacttcgac aacagctccc gcacctccat ctccaccagc tccagaagct ccgcatcgtc
      841 gaccatgtcc gccttgttca ggtacaccac gatgtacggc acccccacct ggcgcgccag
      901 cagaatgtgc tcccgcgtct gcggcatcgg cccgtccgcc gcactcacca ccaggatcgc
      961 cccgtccatc tgtgccgcac ccgtgatcat gttcttcacg tagtccgcgt gcccaggaca
     1021 gtccacgtgc gcgtagtgcc gcttcgaagt ctcgtactcc acgtgcgccg tgttgatcgt
     1081 gatcccccgc gccttctcct ccggcgcgtt gtcgatctgg tcgtacgcct tcgcctcgcc
     1141 gccgaacttc ttcgacagca ccgacgtgat cgccgccgtc agcgtcgtct tcccgtgatc
     1201 cacgtgacca atcgtcccca cgttcacgtg cggcttcgtg cgctcgaatt tgctttttgc
     1261 catgtcgctg tctccagcct tttcgttgat ctttccccgt cgaatcgtcg ggctccgccc
     1321 gcgattcgac gctgactcaa tcgcgcaaaa gcaggggttt acttttgcgc gctacgcttg
     1381 gtgcccatgg tgcggattga acgcacgacc tctcccttac caagggagtg ctctaccact
     1441 gagctacatg ggcgctcgtt cgtaaccgcg aattcggtac accttcctgc aatcctggag
     1501 cgggtgaagg gaatcgaacc ctcgtcgtaa gcttggaagg cttctgctct accattgagc
     1561 tacacccgcg catcaggtaa cagaggtcag gcaacaggca acagaaccgg cgctcgacgc
     1621 gcctccaccg gtatcgccgg cgcttctgtt ccctgtcctc tgatccctga ctcctgaagt
     1681 ggagggggaa ggattcgaac cttcgaaggc agagccggca gatttacagt ctgctccctt
     1741 tgaccgctcg ggaacccctc cgtcgaaacg cgcgattgta gcagttggat gcgaagccgg
     1801 tcaaattcgg ggccaacgcg cgagcgtccg ccgcatgcgt tcgccgcctc ctttagaatc
     1861 agttgcttgc cgccgcaaac ccggcgctga ttgcgccggc gtacgcacaa cgcatccctc
     1921 cttcgaatgc cgcccgccac cgcaagtttc gattacgtcg tcatcggcgc cggatcggcc
     1981 ggctgcgtgc tcgcaaatcg gctttccgag gacggccgct acacggtgct gctgctcgag
     2041 gccggtccgc gcgacaccta tccgtggatc cacatcccga tcggctatgc gaagaccatg
     2101 ttccatccgg tcgtcaactg gggcttctac accgagcccg agccggcgat gaacgggcgc
     2161 aaggtgtact ggccgcgcgg gcgcacgctc ggcggcagct cggcgatcaa cgggctcatt
     2221 tccatccgtg gccagcgcga ggattacgac gcgtgggcgg cggcgggaaa caccggctgg
     2281 agctacgccg acgtgctgcc gtggttcaag cgactcgagc acaacgtgcg cggggcgagc
     2341 gaatggcacg gcgcagacgg cccgctgtgg gcgtcggaca tcggcgctgc ccatccgctg
     2401 atcgaggcga tgatcgccgg cgccggtgag ctcggcattc cggccaatga cgatttcaac
     2461 ggcgccacgc aggaaggtgc cggctactac caactgacga cgcgcaacgg ccggcgttgc
     2521 tcgacggccg tcgcctatct gaagcccgcg cgcgcacggc gcaacctgca tgtcgagacg
     2581 aatgcgcatg cgacgcgcat cgtgctcgag aatcggcgcg caacgggtgt cgtgtatcgc
     2641 caacggggca ccgagcgcac cgttggcgcg aaccgcgaag tgatcctcgc agccggtgcg
     2701 ctgcaatcgc cgcagttgct gcagctgtcc ggcatcggcc ccccatcgct tttgcaatcg
     2761 ctcggcattc cggtgcagca tccactcgaa ggcgtcggcg aaaacctgca ggatcatctg
     2821 caggcgcgcg cgattttccg ctgcacgcag ccgatcacca ccaacgatgc gctgcgcacg
     2881 ctcggcggca agctcgccat ggggatcgac tacgtctttc gccgtcgtgg accgatggca
     2941 atcggcatca accagggcgg catcttcgcc cgcgcaatgc ccggtgcgac gcggcccgac
     3001 gtccagttcc acttcgcgac gctgtcgtcc gacatggcgg gctcgccggt gcacacgttc
     3061 ccggccttca cgatgtcggt gtgccagttg cggccgacgt cgcgcggcac ggtgcgcatc
     3121 aagtcgacga acccgctcga gccgcccgcg atgcaaccgc gctacctgtc gacgcccgaa
     3181 gaccgggcga cgatcgtggc cggcatacgc ctcgcgcggg cacttgcggc gacgcatgcg
     3241 ctgcggccgt acgtcgaaaa cgaataccgg ccggggccgc aggtgcaaag cgacgacgac
     3301 ctgctcgaat tcgtgaagaa cacgggcgga acgatcttcc acccgagcgg caccacgcgc
     3361 atgggaccgg cgagcgaccc gggggcggtg gtcgatccgg agctgcgcgt gcacggcgtc
     3421 gagcgcttgc gcgtcatcga ctgcggcatc atgccgacgc tcgtatccgg caacaccaac
     3481 gtgcccgtcg tgatgattgc cgagaagacg gcagcggcga ttcttgccgg cgacagatga
     3541 gccggctggc tcgagcgagg tcttggcatc gatcgtcggt aagtaatacc atacgatatt
     3601 tggtattact caagggtatg cgcaatggcc actacgataa ccagcaaggg ccaggtgacg
     3661 attccgaagc gagtgcgtga ggcgttgcgg ctcgcgcctg gcgacagcgt cgatttcgcc
     3721 gtgaacccca ccgggcaggt cgtgcttcaa aaggtcgcgc caagatcgac ggccaaacgc
     3781 gatcggttcg agtcggcgcg gggcaaggca caagtcaaat ggcgcaccaa cgacctgatg
     3841 gcgcttttgc gcggtgacga ctgagcaccc ttcgtgctgc tcgtcgacac gaacgttctc
     3901 atcgatgtcc tcgaaaatga tgctgactgg gccgactggt cggtgcgaca gcttcgcgcg
     3961 caggcacagg tgcatgagct cacgatcaac ccggtgatct acgccgaact ttcactgctg
     4021 ttcgaatccg ccgatgcgct ggacgacgcc attgcgaaga tgaaccttca gttccaggag
     4081 ctcccgcgag cagcgctgtt tctcgcaggg cgggccttca tgaaatatcg gcgcgccgga
     4141 ggaaaccgca gtaacgtcct cgccgattac ttcatcggcg cgcatgctgc ggtcgcgggc
     4201 tgcggcatcc tcacccggga cgccggacga tacgcaggct atttcccccg cgttccgctc
     4261 gttacgccgc atacggcctc ggcgcggtga gctagcgctt cgacccgacc cgccaaagga
     4321 gattgaccat ggcctgctgt cacgccgatt tcgccgccct cgaaggcagc gaccgcggat
     4381 tcacgctcgg aatgccgacg ttcacgttcg gcccgggtgt cctcgccgaa gccggcgaca
     4441 atgcggccga gctcggattg aagcgcgtcg cgctcttcac cgaccggcgt ctcgcaagcg
     4501 gcgagcatgt cgcgaaggtg aaggcgtcgc tcgccgccgc gaatgtcgat tgcgtcgtgt
     4561 atgacgacgt cgtcatcgag cccaccgaca aatcgttgca ggaagcggcc cgcttcgcgc
     4621 gtgagagcaa cgtcgacggc tacgtgtcgg tgggcggcgg atccgtgatg gacacgtgca
     4681 aggcggccaa cctgtacgca agccagccgg ccgagttcat gacctacgtg aacgcgccga
     4741 tcggcgccgg gcagcgcgtg cccgggcccg tcaagccgca cattgcctgc cccacgacat
     4801 cgggcacggg ttccgagacg acgggcatct cgattttcac gctcacatcg atgaacgcga
     4861 agaccggcat catctcgcgc cggctgatcc cgacgatcgc gctcgtcgac cccgatgtca
     4921 cgcgctcgct gccggccaac gtcgttgcat cgaccggctt cgattgcatg agccatgcgc
     4981 tcgaatccat caccgcccgc ctctatccac gccgtctcaa tcccgcgcaa gggattcgcc
     5041 ggccggtatc gcagggcgcc aacccgttct ccgatgcact ggcaacgcag gcgctgacga
     5101 gcgtcggcca gtatctggtg cgcgcggtgg acgacgcttc cgacaccgag gcgcgcaccg
     5161 aaatgatgta cgcggcgatg cttgcgggca tcgcattcaa cgcggccggc tgtcatctgc
     5221 cgcatggcct gtcgtacgcc gtgtcggggc tgatcaagga ctggcacgtc gacggctatc
     5281 ctgaaggcaa gtcgctgatc ccgcacggca tgtcggtcgt gctcaacaat ccgtcggtgt
     5341 ggcggcacac cgcattcgcg caccccgagc gtcacctgcg ctgcgcgcgc gcgctcggcg
     5401 gcgcgacgaa cggcgccacg cacaccgacg cgggcgaagt gctcgccgga cgcatcatcg
     5461 agctgatgcg cgcggccggg atgccgaacg gattgacggc actcggcttc ggcgaggcgc
     5521 agatcgacgc gctcgcgacg ggcgccgaac ctcagtaccg cgtcatcaag aatgcgccga
     5581 tcgatgtcga tcgcgacgtg atcaagcgcc tgtttcgcgc ggcacttcga tactggtagg
     5641 cacttcggtc tccgtcgagc cgctccccgc actcgcgcgt ccggcgggcg gcgcgcgttc
     5701 gacgcgctgc gccggaagcc gcaccgtgaa cgtgcttccg ttgccgcgct cgcttgcaat
     5761 cgcaaggtcg ccctgatggc ggagcagcac gtgcttgacg atcgcaaggc cgagtcccgt
     5821 tccaccggtc gcacgtgagc ggctgcgatc gacgcggtag aaccgttcgg taagtcgggg
     5881 gatgtgctcg gcggcgatgc cgatgccggt gtcgcagacc gcgaagacac cgcttccgtc
     5941 gacgtcgatg cgccacgaca gcgtgatcgt tccgccgggt ggcgtatacc ggacggcatt
     6001 gctgacgaga ttgccgaacg cgctcgcgat ctcctcgcgg cttccgatga cagtcgcggc
     6061 gtccgccgcg tcgacgccga tgcggtgcgc ccggttggag agcgcgccgg cgtccgacga
     6121 aaggccggtc agcagcggca ggattgcgaa gcgctcctcg tgcaccggac cctgttcggt
     6181 ttcgagcgcc gacagcgtca gcagatcggc aacgagacgt cgcatgctcg tcgcctgttc
     6241 ctgcatcagt tgcaggaagc gcgcgcgctc gcgcatcccg acgtcgacat cctgcagcgt
     6301 ctcgatgaag ccgctgatga ccgtgagcgg cgtcttgagc tcgtgcgaga cgctggcgat
     6361 gaaatcccgg cgcacgcgcg cgagcgcttc catcccggtg atgtcgcgcg ctatcaggag
     6421 cttctggtcg tgcgcgaacg gcacgagctg cagcgccagc gtgcacgcag cctcgcggtc
     6481 ggattcgacg acgaccggtg cgccataatc gccgcttgca acatagcggc gaaattccgg
     6541 ctggcgcacg aggttcgcaa tcggttgtcc gcggtctttc gcgagatcga gaccaagctg
     6601 cgcaagcgcg cgcgcattcg cccagtcgat gcggttcgcc gcgtcgagca cgacgatgcc
     6661 gtcgggaatc gcggtcgccg cctggcggaa acgctcgatg acctgccgca gttcgcgctc
     6721 gtaggcgttt cgcgcgcgaa cgcggcgatg gatcgatccc aacggcgccg cccaggcgcc
     6781 gcgaccctcc ggtacgtccg tgtccggcgc agcgcgggcc cacacgtcca gccgatgaag
     6841 atgaaacaga tgaaaggcga tgatcgccga tgcgccgagc gccagcacgg cgagcgcgag
     6901 cgcagccccg ccggcggcgg cgcacagcac ggtaagcaac gcgacggcgg ccggcgcacg
     6961 cagcgcgcgc caggcgaagc tgctcacctt cgacccgcgt ccgcttcagg cgacgcatct
     7021 gcggccggat gcgaaacgaa acggtagcca ctgccgcgca cggtttcgat cgcagacgca
     7081 gcgcccgacg cctcgagcgc caggcgcagg cgccggatgt gcacgtcgac cgtccgctcc
     7141 tcgatgtaga cgtggtcgcc ccagacgccg tcgagcagtt gcgtgcgggt gagcacccgg
     7201 tcgggtcgcg ccatcagaaa gcgcagcatc cggaattcgg tcgggccgag cggaatgtcg
     7261 gtgcccgcga cactgacgcg atgcgcaacg ccgtccagcg tcagctttcc cgaacgcagc
     7321 gtgtcctgcg cggcttcggg cgcgcgccgg cgcagcaccg ccttgatgcg cgccttcaac
     7381 tcgcgcgggc tgaacggctt cgtcacatag tcgtcgaccc aggcctcgag gccggcgatc
     7441 ttgtccgctt cctcggtgcg cgcggtgacc atgatgagcg gcaattcgcg cgtgcgccgg
     7501 tcggcgcgga gttgccgcgc gagtgccagc ccggattgcc ccgggagcat ccagtcgagc
     7561 agcacgagat cgggcagccg ctgtgcaagc gcgcgttgtg ccgcggcggc gtcgagcgct
     7621 tccagcacct cgtagccggc gtcctgcagg ctgaatttca gcagctcgag gattgccggc
     7681 tcgtcctcga cgacgagaat cgacggcgtc aaggctttgc caccgatgcg ctcagcgccc
     7741 ggccacttcg gcgcggattt cgtcgggaga tgcatgccgg acgtccttgc ccatgaccac
     7801 ctggaccacc gcctcggcga tgttcttcgc atgatcgccg atgcgctcga tcgactttgc
     7861 cacgaacacg atctcgagcg ccggcgtgat cgtgcgcgga tcctccatca tgtagctcac
     7921 gagctgacgc aggatcgacg cgaacgcggc atcgatctcg gcgtcgcgcg cgacgatctc
     7981 ggcggcggcc accgcatcga ggcgggcgaa ggcgtcgagc gcctcgcgca gcatcgtcgt
     8041 cgcgcgatcg agcgcgcgca gcacatcgta atggcgcacg cgcgcaagcc gctcgctgcc
     8101 gcgcgtctcg gcggccctgt acgcaatctt cttagcctcg tcgccgctgc gctcgaggtc
     8161 gttgacgatc ttgctcaccg tcagcacgat ccgcaggtcg acggccgcgg gctggcgccg
     8221 cgcgatgatc tgcgcgcatt gctggtcgat cgccatctgc aactgattga tcgccgcttc
     8281 gtcggccgcg acggcgccgc cgcgtgcgcc gtcgtcgtcg ttttcgaggg cgtcgaccgc
     8341 gcggcgcaac tgacgctcga ccgtgccgcc catcgacagc acgccggaac ggatcgcctc
     8401 catctcggcg tcgaactgct tgttcgtatg ctcggtcatc gcccgtccta tccgaacctg
     8461 ccggtaatgt aatcctccgt ctcccggcgc gccggcttca ggaagacctg atcggtgggg
     8521 ccgaattcga cgagctcgcc gagatacatg taggccgtcc tgtccgacac gcgggccgcc
     8581 tgctgcatgt tgtgcgtgac catggcgatg gtgtagtcgg ccttgagctc cgaaatcagc
     8641 tcctcgatgc gcgcggtgga gatcggatcg agcgccgagg tcggctcgtc gagcagcagc
     8701 acctcgggta gcacggcgat ggcgcgcgcg atgcaaagcc gctgctgctg gccgcccgac
     8761 aatgccatcc ccgactgcgg gagcttgtcc tttacctcgt tccacatggc cgccttcgtg
     8821 agcgcccatt cgacgcgctc gtccatggcg cgcgccgaca gccgctcgta gaggcgcacg
     8881 ccgaacgcga cgttctcgtg gatcgacatc gggaacggcg tcggcttctg gaacaccatg
     8941 ccgaccttcg cgcgcagcag attgacgtcg gtcgacgcgt cgaggatgtt cacgccgttg
     9001 aaccggacct cgccggttgc gcgctgcccg ggatacagcg aatacatgcg attcagcgtg
     9061 cgcagcagcg tcgatttccc gcagcccgac ggaccgatga acgcggtgac ctcgcgctcg
     9121 gcgatgtcga ggtcgacatg cgcaagcgcc tggaagccgc cgtagaagaa gtccagattg
     9181 cgaatcgaca tcttcgcccc ggcggtgctc gccgcatcgc gcggcgtcgt cacggcaggc
     9241 gagcgaacgg gcacgtcgat gagcggcatc ggcatcatcg caatgccgac ggccggaaca
     9301 cggctcgtgc cacgatgttg agcgtcagca ccgccagcgt gatcagcaat gcacccgccc
     9361 acgccagcgt cttccagtcg tcgaacgggc tcatcgcgaa ctggaagatg acgacgggca
     9421 ggttggcgat cggcgcattc atgtcggtgc tccagaactg attgttgagc gcggtgaaga
     9481 gcagcggcgc cgtctcgccc gacatgcgcg cgacggcgag caggatgccg gtgatgacgc
     9541 caccgcgcgc ggccttgagc gtgaccttca cgatcacctt ccatctcggt gccccgagcg
     9601 ccacggcggc ctcgcgcagg ctgcccggta cgaggcgcag cgtgttctcg gtggtgcgca
     9661 cgaccaccgg aatcgcgatc agcgccagcg ccagcgagcc ggcccagccc gaaaagtggc
     9721 cgatctgcag cacgtagacg gcgtagacga acagaccgag gacgatcgac ggcgccgaca
     9781 gcagcatgtc gttgacgaaa cgcgtcgcct ccgcaagcca gccgctgcgg ccgtactcgg
     9841 cgagatagat gccggcgagc atgccgatcg gcgtgctgac gagcgtcgcc gtcgcgacga
     9901 tcagcacgct gccgaagatc gcgttgcgca atccgcctgc cgcgcccggg ggcggcgtca
     9961 tctgcgtgaa cagcgacggc gacagcgcgg cgagcccatt cgcaaaaagg gtcgcgagaa
    10021 tccacaacag gaagagcatc ccgagcgcca tcgtcgccag cgacatcgcg aggttgaacc
    10081 ggctcacgaa aagacggcgg cgataacggc gcgtcatcgc cgcgaccgtt ccgtgagagc
    10141 cgccgggccg ccccagggcg cgaaccgcgc cgcctcgagg ggcagcgcag cggcgacgcc
    10201 gcaagcgtag ggggaccatg caagcggcgc ggtcggacgt gtcacgccgg cgcaccttcg
    10261 ccgcgcgtga gccgcgcgat cagcagcctc gaggcggcga gcacgatcag cgtcagcacg
    10321 aacagcacga gcccgagcgc gatcagtgcg ccgcggtgca ccggatcggc cgcctcgttg
    10381 aactcgttcg cgagcgcgga cgcaatcgaa ttgccgggct ccagcagcga cgcgcggatg
    10441 cgataggcat tgccgatcac gaacgtcacc gccatcgttt cgccaagggc gcggccgagg
    10501 ccgagcatga tgccgccgac gacaccggcg cgcgtgtacg ggagcacgac gctgcgcacg
    10561 acctcccacg tcgtgcaacc catcgcgtac gcagattcct tcagcaccgg cggcacgatg
    10621 tcgaacacgt cgcgcatcac cgccgcgatg aacgggatca ccatgaccgc caggatgatg
    10681 cccgccgtca gcacgccgag tcccgtcggg gcgccctgga acaacgggcc gatgacccac
    10741 gcgttgccca gcgtgcctgt gagcagcggt tccacgtggt cggcgaagaa cggcgcaaat
    10801 acgaagaggc cccacatccc gtagatgatc gatggaatcg ccgccagcaa ttcgacggcg
    10861 gtgccgagcg ggcgtttgag cgacggcgga cacatttcgg tcaggaagag cgcgatcccg
    10921 aagctcaccg ggacgccgat cagaagcgcg atcgccgatg tcacgatcgt gccgtagatc
    10981 ggggcgagcg cgccgaactc gctcgtcacc ggattccagg cgttgctgat gaaaaagcgg
    11041 gggccgaatt tcgacagcgc cggccacgcc gcgtaaccga gcgcgatcac gatcgcgacg
    11101 agcagcatca gcaccagcgc ggcgaacgca cgcgtcgcgt gcgcgaacaa cgcgtcctgc
    11161 cgccgaccgg ccgcggcacg gcgcatccgg cgttgcgcat tcgcgaccgg cagtacgtcg
    11221 atgctcatcg aactcatcgt gcgccggcgg ttactccccg ctcgccggcc agatgggctt
    11281 gccgctcgca tcgttgattg tcgccttcca cgacgactcg acgagctcga cgagattcgg
    11341 cggcagcgca acgtagtcga gctccccggc cagcttctga ccgttcctga acgaccaatc
    11401 gaagaatttc agcacttgca ccgcgttttg cggcttctgc tgggttgcgt gcagcaggat
    11461 gaagctcgcg ccggtgatcg gccatgcgga agcacccggc tcgtcggtaa ggatccggta
    11521 gaagcccggc gcgttcttcc agtcggcatt ggcggccgcc gcctggaatg tgctgtcgtc
    11581 gggtgcggga tactggccat cgcggttacg cacgagcgca tgcgccatgc cgttgcgctt
    11641 cgcgtacgca tattcgacgt agccgagcgc gcccttgatg cgctggacgt aggccgccac
    11701 gccttcgttg cccttgccgc cgacgccctc gggccaggcg actgccgtgc ccgccccgac
    11761 cttcgtcttc cattcgatac tgaccttcga caggtattcg gtccagagga acgtcgttcc
    11821 cgagccgtcg gaacggtgca ccacggcaat cgcttcatcc ggcagccgga tcccggcgtt
    11881 cagctcggcg atcgccttgt cgttccaccg cttgaccctg ccgagataga tatccgcaag
    11941 caccggtccc gtgagcttca gcgcgccggg tgcaacaccg tcgacgttca tcaccggcac
    12001 cacgccgccg atgattgccg gaaattgcac gagccctgcg cgcttgagct cctcgggcct
    12061 gagcggcatg tcggacgcgc cgaaatcgat cgtcttcgcc ttgatctgcg cgatgccgcc
    12121 gccggatccg atcgactggt agttcatgcc gatgcccgtc gtcgtcttgt acgcagccgc
    12181 ccatttggcg tagaccggga acggaaacgt ggcgcccgcg ccggtgatgt cggccgcgac
    12241 ggccggcgcc agcggcacgg cgagcgccgc ggcgatcgcc gtcaagcccg ccttcatgct
    12301 tcgcattcga tgtcctcttc gttgatgaca ccgtgcagtg tggcggcgca atgtgacagc
    12361 acgatgacaa caccggccgc gcaaggccgg aagggggaac gcggggaacg ctgaggcgcg
    12421 gcgcgaggcg ccgcaaactc aatgctgcgc cgtggcgccg gcgagctttt cgacgagatc
    12481 gtggttggct gcgagctcgg cggccgtgcc cgagtagacg accttgccgt cgtcgaggat
    12541 gtaggcgcga tcggcgatgc cgagcgacag cggcacgttc tgctcgacga gcagcaccga
    12601 caatccctcg tcgcgcatcg cgcgcaccac ctgcatcact tcctgcacga tgagcggcgc
    12661 caggccctgc gacggctcgt cgagcagcat gacgcgcgga ttgagcagca gcgggcgcgc
    12721 gatcgacagc atttcctgct cgccgcccga gagctggcgc ccgagttgcg tcttgcgctc
    12781 gccgagccgt ggaaacagct tgaagacgcg gtcgagcgtc catgcgccgg ggcgtgcctc
    12841 gggcaccgtc aggttctcga gcacggtcag gttgccgaag atcttgcggc cttcgggcac
    12901 gtagccgaca ccgagccggg cgatcctgta ggcgggccag cgcgtcgtgt cctggccgag
    12961 catgtggacg ctgccttcgc gcacggcggt aagccccatc aggctgcgca tcgtcgtcgt
    13021 cttgccggca ccgttgcggc cgagcaccgt gacgatctcg ccctcgtcga gacgcagcga
    13081 cacgccgtgc aggatatggc tcttgccgta gaacgcgtgc acgttgcgcg cctcgagcac
    13141 cgccgcgcga tcgacggggg ggagcacgcc accgctcatg cgtcggtccc caggtacgcg
    13201 gcgcgcaccg cgtcgttgtg cgaaatctcg tcggcgctgc cttcggcgag cacccgtccc
    13261 tggtcgagca ccgtgatgcg ctgcgcgatg ccgaacacga tgctcatgtc gtgctcgatg
    13321 aacaggatcg acagcttccg ctgcgcgtgc aggcgccgca cgaggtcgac catgtgatcg
    13381 gtctcggtgt cgcccatgcc ggccgtcggt tcgtcgagca ggagcagccg cggattcatc
    13441 gcgagcgcca gggcgatctc gacgacacgc tgatcgccgt gtgcaagctc gccgaccacc
    13501 ttgtccgcct tcgtttcgag tccgacgaca tcgaggagct cgtcgacctg gcgcgcgatg
    13561 tcggctcgca tcgcccgtgg cagccacggc cgcccgttga gccccgcggc ggtttccgca
    13621 ccgacccgca cgttctcgta gatcgtcagg tcgggaaaca cctcggtgat ctggaacgtg
    13681 cgcacgatgc ccttgcgcac gcggtgcacg acggtgcgat cgcggatgtt ctcgtcgtcg
    13741 aaccagatgt cgcccgacgt cggcggcacg aatccggtga tgaggttgaa gaatgtcgtc
    13801 ttcccggcgc cgttggggcc gatcaccgcg cgaagctcgc cctcgtccat cgagaacgag
    13861 acgtcctgca ccgcctgcag cttgccgaag tgccggctca cgttggcgag ccgaagcagg
    13921 cttgtatgat ccccccacgc ttgcggcttc gccgctgcgc tgccccccga gggggcgcaa
    13981 ttcgcgcctt gaggcggctc tgcagcgctc attgggtccc cccgcgcttc gcgccttggg
    14041 acggcccggc ggcgctcatt tcatttcctg cttcagcggc gcaggcggcg gctcgatcgt
    14101 tgccgtgctc cgttcgatcg ccgctcgcgg gccggtgacc gccaccgcga gctcgcgtcc
    14161 gcgccgccgc accaatccga gaatgccctt cgggaacagc agcacgatca gcacgaacag
    14221 gaggccgatc gccgtcatcc agttgtccgt caggctcgac aggtagtcct gcgcgacgac
    14281 gaagatcgtc acgccgacga gcggccccca gaacttccgc atgccgccca gcacgatcat
    14341 gatcacgaag ttgcccgaca ggatccagtg cagatcctgc ggcgacgcga agttgaagag
    14401 catggcgttc agcgtgccgg ccagcgccgt cagcgtgccc gagatcgcga atgccgccca
    14461 gacgtagaag tcggtgcgta cgccgaggaa ctgtgcgcgg cgccgattct cgcggatggc
    14521 gacccacgta tgaccgagcg gcgacgcgag cagcacgcgc atgatcacca cggcgacggc
    14581 gaagcacagc agaacgaggt aatagtattt cgactcgctc tgcgcgtcgt agacgaagct
    14641 gccgagatgc agcggctggc gccggaatcc ggccagcccg tcctcgccgc ccgtcacgtt
    14701 gttccagcgg acgaccacga aatagaaaag ctgcccgatc gcgatcgtga tcatcgcgaa
    14761 gtagatgccg cgccgtcgca tgacgagcgg tccgagcagc gtcgccgcga ggcccccgac
    14821 gacggtcccg accaggattg cgagcggcgt cgagggcacc aggtatttga gcgtcattcc
    14881 cgcgccgtac gcgccgagcc cgaagtacgc cgcgtggccg aacgaaagcc cgccggtgaa
    14941 gccgagcacg aggttgagcg caagcgctgc gatgccgtag atcaggatct tgctgccgag
    15001 cgccgcatag ccgccgacgt agccgagcca caacggcacg gtcagcaatg cgatccacag
    15061 cacgatctcc ggcgcgtgcc ggcgcatcat tcgaacagcc cctcctgtcc gagcaggccg
    15121 cgcggccgga tcacgaggac gatcagcatg atcagataga tgaccgcctc gcttgccgcc
    15181 ggatagtagg ttgtcgtgat gccggccgcg acgccgatga gcagtccgcc gagaatgctg
    15241 ccgacgaggc tgccgacgcc gccgacgatg atcgcgacga agctcggcat gatgaggctg
    15301 cttcccatcg gcggcgtgag cccgaggatg cccgccgcga gcacgcccgc gagcccggcg
    15361 agcaggcagc cgagcgcgaa gttgagcgcg cgcagcagat agatgttgac gccgagcgcc
    15421 gagaccgtct cgagatcgaa ggttccggcg cgaatgcgga tgccgagccg cgtgtagcgc
    15481 aacaggaggt acagcccgag catcacggcc atcgtgatcg caacgaccag cagccggtaa
    15541 ccggtgatga agaacagcac gctgctgatc ggcgaattga gcgcggacgg cacctggaac
    15601 ggcaggccct gtgcgcccca gatcgtgcgc gtcaggtcct cgatgatgag tgccaggccg
    15661 aacgtcagca gcagcgtgta cagcggatcg ggccgcttgt agagcggacg gatcagcacg
    15721 cgctcgacac cgagcccgat gatcgcggca cccacgggac ccacgacgag cgcgccccag
    15781 aagccgagat acggcgcgag cgagtacgca aggtaggcgc cgatgacgag aaagcccccg
    15841 tgcgccatgt tgatcaccga gttgaggctc aggatcagcg cgaggccgag agccatcagc
    15901 gcgtagaaga cgccctggat cagaccgttg aacgcgttga acagcagaag ctgcatgcgg
    15961 ctcctcttgc gcttcgatgt tgcctcgagc gccggcggac gggtgtccgc cggctaacgc
    16021 gtcatcatcc gcgcgtcatg atccgtccac gcaagcggcc cggccgccgg ggcatgcaac
    16081 ggttcacacg acgcgtccat ccgatccgcc gcccgatcag gccggatagt cgagcttgca
    16141 gccggtctcg gtgaccggca gcgccgcctt gtcgccggga acgatgctcg caaccttgaa
    16201 caggtcggga tactttccgt tcgtgttgat ctcgcccgga aactcgctcg acatgagctg
    16261 atggtcctgc g
//
LOCUS       DASSVX010000058        16694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_735491, whole genome shotgun sequence.
ACCESSION   DASSVX010000058 DASSVX010000000
VERSION     DASSVX010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 16694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16694
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735491"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFO53_02675"
     CDS             complement(<1..588)
                     /locus_tag="VFO53_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET8875207.1"
                     /translation="MRPFTFSCALAAAVLAVSVPAAAQSPLIVAAKSRPSARSAAPAP
                     SVPETPANPPVVLQTPGAPAAPTIAAASWVLVDTLSGQTLGASNADERRDPASLTKLM
                     TAYVAFEALRAKSIAPAQMVSVSERAWKAGGSRMFIEPRKPVSVDELLHGVIIQSGND
                     ASIAIAELVGGTEEGFVDRMNQEAAKLGMTGTHFTN"
     gene            722..1612
                     /locus_tag="VFO53_02680"
     CDS             722..1612
                     /locus_tag="VFO53_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET8875208.1"
                     /translation="MMRPLAALCIGASCLLFTLEAMPHEPDAEEVALGSLVDAELAFA
                     RMGRERGVHAAFLANFAADGVALEPAPVRVQQAFRERAAPADPLALRLDWKPAQAGVA
                     RSHDFGYTTGPYTLSSAANPQTRREGAFFSVWQRSPAGRWEVILDAGIGTAGTVDFVA
                     LGAAPRPGYAGPARPAAARRDLLAQETSGFGAAGAALTPNGYARLLADDARLHRNGAA
                     PIASRGAIARATAQRIQRVTWTPIDARVARSADMALTWGRYRETDRAAHEQDGYYAHL
                     WLRDRAGRWRLAYDVALPRS"
     gene            1675..3084
                     /locus_tag="VFO53_02685"
     CDS             1675..3084
                     /locus_tag="VFO53_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875209.1"
                     /translation="MKRSCVSLLVLVAVLCASSALAAFHLFRVDQVYSNADGSVQYVV
                     MREVTGSDGENFWQGNSLTTTGAGGMQQFQFPANLPSASTASRSVLVATSGFAALGLV
                     TPDYTIPAGFVPRAGGTLNYAGVDQIALPALPADGVTAVDRNGNQVAATPKNFAGATA
                     TLTTPTPPAATAPDLDQHGLTGSWFEPATSGQGFEVEFYPDLVAAGTALVQGAWFTFE
                     VAPAGGADRQRWYTFNGHAQSGDDDVNITIFQNVGGNFDAPPVTNGVAVGSGTLSFDT
                     CTSGSLNYAFSDGSGRSGTIPLSRITPNVTCATGTAAPTTNADFALSGNWFDPATSGQ
                     GFVFEVNPIVSVVSFAWYTYSPTGQMAGAAGQRWFTGSASYAPGNRMIPLTLFETTGG
                     VFDQPTPAAQTSTPVGSAVVNFTSCSTAQLQFNFTGGGNAGHAGTISLARVGPVPPGC
                     VNSSSDASMQPGYGGGYGP"
     gene            complement(3129..4385)
                     /locus_tag="VFO53_02690"
     CDS             complement(3129..4385)
                     /locus_tag="VFO53_02690"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HET8875210.1"
                     /translation="MRILVLGGGVVGVTSAWYLARDGHDVTLVDRQPGPALETSFANG
                     GQISVSYAEPWANPGAPLKILKWLAHDDAPLLFRPRLDWRQWRWGLAFLLECLPSRTR
                     RNTIQCLNLALYSRDCLKVLRESTGIAYDQLERGILQFYTDAAEFDRGVHAAELMRVY
                     GCDRDVKTPDECVAIEPALAQCRDKVAGGIYTATDESGDAQRFTTELARLAAAEGVAF
                     EWSTEIQALSPAGDAIERVRIVREGARFDTLAADAYVMALGSYSPFLLAPVHVPCNVY
                     PAKGYSATIDIGGHRGAPTVSLTDFDWKIVLTRLGQRLRVAGTAELNGWDTSLNQTRC
                     EALTARTFELFPDAGDRDSVRYWAGLRPATPSNAPLIGRTRYRNLFLNTGHGTLGWTM
                     ACGSARALADVVAGRKPDVDFRFSGS"
     gene            complement(4485..7493)
                     /gene="gcvP"
                     /locus_tag="VFO53_02695"
     CDS             complement(4485..7493)
                     /gene="gcvP"
                     /locus_tag="VFO53_02695"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HET8875211.1"
                     /translation="MPEAAQRNETTDATSVPALQSLERHDEFLARHIGTPRSAQDAML
                     REIGFASRAALMSAIVPSGIRSTEPLALPPPLGEAEALAKLRRIASRNRVLKSFIGQG
                     YYGTHMPGVIQRNVFDNPAWYTAYTPYQPEISQGRLEALVNFQTMVTDLTGLAIANAS
                     MLDEATAAAEAMTLCQRVSTSKRRAFFVADDVFAQTIDVVRTRAAPLAIEVVVGPVSE
                     AAGADAFAALLQYPGADGDVRDYRALVDALHASGTLVVAAADLLALTLLSPPGEWGAD
                     VAVGSTQRFGVPMGYGGPHAAYMATRDAYKRALPGRLVGVSVDAAGAPAYRLALQTRE
                     QHIRREKATSNICTAQVLLAIIASLYAAYHGAEGLTAIARRTRRLTAILRAGLARLGF
                     DVATGIAFDTLVIDTGERTGAIAARALAAGCNLRLRDGASLGISLDETTTRHDVAALW
                     RIFTGGDVALDVETLDADVDDAYPPEIARASPFLTHPVFHRHRSETQMLRYLRRLADK
                     DLALDRTMIPLGSCTMKLNATSEMLPVAWPEFGALHPFAPADQVTGYRELVDDLEHML
                     CAVTGYDAVSLQPNAGSQGEYAGLLMIRAYHASRGEAHRDVCLIPASAHGTNPASAQL
                     AGMRVVVVGCDRDGNVDVAELEAKAREHRDRLAAIMVTYPSTHGVFEPGIGHVCDIVH
                     AHGGQVYVDGANLNALVGLAAPGLFGADVSHLNLHKTFCIPHGGGGPGVGPVACRAHL
                     APFLPGHRYLRVAPPHPHPLPAGAREPSDHEAGDGSVATNAVSAAPYGSASILPISWM
                     YITMMGARGLRDATESAILAANYVAKRLAPHYPVLYSGPDGLVAHECILDLRPLKPLA
                     GVEVEDVAKRLIDYGFHAPTMSFPVAGTLMVEPTESEPKEELDRFVAAMIAIRNEVRA
                     IENGAFDRADNPLKHAPHTAAAVSADTWTHAYTRQQAAWPLATLAGDKYWCPVARVDN
                     VHGDRNLFCSCVPVDEAGAQAPIPLTPALA"
     gene            complement(7486..7869)
                     /gene="gcvH"
                     /locus_tag="VFO53_02700"
     CDS             complement(7486..7869)
                     /gene="gcvH"
                     /locus_tag="VFO53_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET8875212.1"
                     /translation="MNVPDGLKYADTHEWLRAEDDGTVTIGITDHAQAALGDLVYVDL
                     PAAGRTVAAGDACAVVESVKAASDVYAPIAGEIVASNTSLSGSPEAVNEDAYAAWLFR
                     LRPADASALSAFMDADAYKRLIADA"
     gene            complement(7903..9009)
                     /gene="gcvT"
                     /locus_tag="VFO53_02705"
     CDS             complement(7903..9009)
                     /gene="gcvT"
                     /locus_tag="VFO53_02705"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET8875213.1"
                     /translation="MTSTTALNAVHRALGARMVDFGGFDMPLHYGSQIDEHHAVRTGA
                     GMFDVSHMRVVDLHGNGGDAPRTFLRHALANDVGKLAKPGRALYSCLLREDAGVLDDL
                     IVYYLRDDWFRLVVNAATAGKDVAWLRALAGERAPGLRIVPRDDLAMLAVQGPNARDN
                     VWRAVPGSEAATKDLAPFNAASTATPAGELFIARTGYTGEDGFEIVVAAAGAATLWQA
                     LRDAGVTPCGLGARDTLRLEAGMNLYGQDMDESVSPLESGLGWTVDMKGERDFVGRAA
                     LLARPVSRRLAGLVLQDAGGVLRAHQSVHTTHGTGEITSGTFSPTMKKSIALARLPAA
                     VADGDGVRVDVRGRLLGARVVKPPFVRRGRVLVA"
     regulatory      complement(9056..9170)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(9171..9261)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(9335..9595)
                     /locus_tag="VFO53_02710"
     CDS             complement(9335..9595)
                     /locus_tag="VFO53_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET8875214.1"
                     /translation="MADQLEDMMKKNGCNACHAEDKKILGPAYKDVAAKYKGDSTAVA
                     KLTEKVKKGGSGVWGPVPMPPNAQVSDADIKKMVEMILALKK"
     gene            complement(9726..11759)
                     /locus_tag="VFO53_02715"
     CDS             complement(9726..11759)
                     /locus_tag="VFO53_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET8875215.1"
                     /translation="MANRLAGASSPYLRQHANNPVAWLPWGEEALTLARDENKPILLS
                     IGYSACHWCHVMAHESFEDPAVAAVMNERFINIKDDREERPDLDQIYQTAHALMTRRS
                     GGWPLTVFLTPDGAPYFAGTYFPKQPRYGMPGFLDLLPRIADAFHDQGPAIAEQAERL
                     SRALDALEPTHPANALPSGAPAQELARLTQAFDPVDGGFGGAPKFPHAAELDFCLRAW
                     RLRGDDGARTIVRTSLERMAAGGIFDQLGGGFCRYSVDARWSIPHFEKMLYDNAALLA
                     LYADAARALDEPRFGAVAKAIVSWLVREMRAPDGAFYSSLDADSEGVEGKYYVWTRDE
                     VRAVLSGDEWTVAAAHYGLDGPPNFEQHAFNLRVAASIGDVAAKLGASPADVEARLSR
                     ARAALLAARAARVRPGLDDKVLTSWNALMIAALARAARTLCEPAFADLAFAALDTLVA
                     TAWRDGRLYATRHRDVASLPAYLDDHAFLLAALLEAMQTRFRLRDFSFAVELADALLE
                     RFEDRERGGFWFTSHDHERLYHRTKPLHDNATPSGNGIAAQSLIMLGHLAAEPRYVDA
                     AERTLKLFAPALAESGGAQSTLLVALERLLAPPSTVVLDGERATTQAWQRMLERKYRP
                     DVCIVDIAGAGAPAPLRKGDAPADGARAWICRGLQCLPPVAAVDAIILALDAS"
     gene            complement(11845..13488)
                     /locus_tag="VFO53_02720"
     CDS             complement(11845..13488)
                     /locus_tag="VFO53_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875216.1"
                     /translation="MQDVAAPPLVDADATGEADPGPESLPELAFNDAASAGRWMKALP
                     LSAVGQAYEMLLGQLRALTASEMGPRDRATIAELAREPVAHLHTELARRYAGRPQPSD
                     EREADAADQAVALWQALWIQYSVCLRPLLEGSTELKGVKAKLLQRALYVGKQLIVVHG
                     LARRVPDAELWQELHAYYRLAEMLDCTTASVSDHLMPGAEGLSCYSTYSHTLLLALAD
                     PYALSARQIELTDRWLGLWARKLFPYAKQRETEGPIIVVDLEGAHGAMLAATTASAQG
                     PSWRFGYPAKLSTSVRGRLKRLASGASPADLQLGEDVSADAASALLAHLDAQWHRVGG
                     GANRKRSKAVEIAAGGIEGAYFRVGGRSFRHVDPLGRDSDPTKYLQTVGGISDFDRRK
                     DEAERSWPWEKWYGSCEWREASLRHQGASGCHWFLDQLVVVREEGETRLGHVSRVAVD
                     ADGAITIAIRLWAGAPKVFAVRLVNPNRTEEPPVAALILDETPEEPTTLVMSPRAFIP
                     NRIMRCDEPGQMRKFRLTHILQRGGDFERVAFEAVDPDF"
     gene            complement(13923..>16694)
                     /locus_tag="VFO53_02725"
     CDS             complement(13923..>16694)
                     /locus_tag="VFO53_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3400 domain-containing protein"
                     /protein_id="HET8875217.1"
                     /translation="RRETLTIPGDRFRRAGLGKDVTDKFLAGLPGVQKEGCDGIVVSA
                     RFVLHRMPPSIRTVCLEFFGEARESTPAIVEIKRFLDDTARRPDGERVQLAGLEHLDA
                     RYVKAVGYATKAKRHGYPKMVLIGDIVGERDEAVAKAASDVIRIANARGGEGFVAVSA
                     EARKKFWLDRSRTAAIAKHTNAFKINEDVVIPLDRLGDYTDGVERLNIAMSIGNKLVL
                     ADRLTQFFAEPDVEHLWRPGSEARPTDTELAAALDEARALVSEVRAQWQYLLDRLDET
                     FADLQSHAVVVSWKRELRSPLERIFAGRAFAPLFARCDEIHREVLRSRVFIALHMHAG
                     DGNVHTNIPVNSDDYGMLQQANAAVERVMALARALNGVISGEHGIGITKVAFLTDDEL
                     APFADYKRRIDPEGRFNRGKLVRSRGLPSDLTNAYTPSFSLIGHESLILEQSAIGEIT
                     TAIKDCLRCGKCKAPCATHSPRANLLYSPRNKILATSLLTEAFLYEEQTRRGVSLRHF
                     DEFGDVADHCTICHKCLNPCPVDIDFGDVSIAMRNLLRREGKKKFNVGTAAAMFFLNA
                     TDPATIKLAKMAMIDFGYRAQRLAYSIVKNAGIARAQTAHPPSTTGRTPVKAQVIHFV
                     NKPMPKGVPKRTARALLDVEDPTIIPIIRDPSKVNDDSDAVFYFPGCGSERLFSQVGL
                     ATQAMLYHVGAQCVLPPGYLCCGYPQTAAGNHDKGQEIITDNRVLFHRVANTLNYLDI
                     RTVIVSCGTCLDQLQKYEFDKIFPGCRLLDIHEYLLEKGVALEGVEGVRYMYHDPCHT
                     PMKVYKPLAVVSQLMGTDVPLNDRCCGESGTLATTRPDISTQVRLRKEDEMRAGADAL
                     RADGFTGQVKVLTSCPSCLQGLSRFEDDAGTDADYIVVELAKHVLGESWMADYVAKAN
                     RGGIERVLV"
BASE COUNT         2664 a         5664 c         5684 g         2682 t
ORIGIN      
        1 attggtgaaa tgcgttcccg tcatgccgag cttcgccgct tcctggttca tcctgtcgac
       61 gaagccttcc tcggtaccgc caacgagctc ggcaatcgca atcgacgcat cgttgcccga
      121 ctggatgatg acgccgtgca ggagctcgtc gacgctcacc ggcttgcgcg gctcgatgaa
      181 catccgcgag ccgccggcct tccaggcgcg ctccgacaca ctcaccatct gcgccggcgc
      241 gatcgatttc gcgcgcagtg cttcgaacgc gacatacgcg gtcatgagct tggtgagcga
      301 tgcaggatcg cgacgctcgt cggcattcga cgcgccgagc gtctggcccg acagcgtgtc
      361 gacgagcacc cacgacgcgg cagcgatcgt cggtgcggcc ggcgcaccgg gcgtctgcag
      421 cacgacgggc ggattcgccg gcgtctcggg aaccgacggc gcgggcgccg cggagcgtgc
      481 gctcgggcgc gatttcgcgg caacgatcag cggggactgc gcggcggcgg gaaccgaaac
      541 ggcgaggact gcggcggcga gcgcgcagga aaacgtgaac ggacgcatgt agttcaagcg
      601 gaaacggcac attatactga agcgaatcgt ggaaaggcgg cgtttgcccg gattgcatct
      661 catcatgaaa cccgggcggc ggcgaggacg catgcgagcg catgcaaaac gcacatcgtt
      721 gatgatgcgc ccgctcgcgg cgctttgcat cggcgcatcc tgcctgctct ttacgcttga
      781 agcgatgccg cacgagcccg atgccgaaga ggtggcactc ggctcgctcg tcgatgccga
      841 actcgcattc gcacgcatgg gccgggagcg cggcgtgcat gccgcgttcc tcgcgaattt
      901 cgccgccgac ggcgtcgcac tcgagcccgc accggtacgc gtgcagcagg cgtttcgcga
      961 acgcgccgca cctgccgatc cgcttgcgct gcggctcgac tggaagccgg cacaggcggg
     1021 cgtcgcacgc agccacgatt tcggctacac gaccggacct tacacgctgt cgagtgccgc
     1081 caatccgcag acgaggcgcg agggcgcgtt cttttcggtg tggcagcgca gtcccgcggg
     1141 acgctgggaa gtgatcctcg acgccggcat cggcaccgcc ggcacggtcg acttcgtcgc
     1201 actcggcgcc gcgccccgcc ccggctacgc gggtccggca cggcccgccg cagcacggcg
     1261 cgacctgctc gcacaggaga cgagcggatt cggcgccgcc ggtgctgcgc tcacccccaa
     1321 cggctatgcg cggcttcttg ccgacgacgc gcgattgcac cggaacggcg cggcaccgat
     1381 cgcatcgcgc ggcgcgattg cgcgcgcaac cgcgcaacgc atccagcggg tgacgtggac
     1441 gccgatcgat gcacgcgtcg caagatcggc cgacatggcg ctcacgtggg gccgctaccg
     1501 ggagaccgac cgcgccgcgc acgagcagga cggttactac gcgcacctgt ggctgcgcga
     1561 tcgcgcgggc cggtggcggc tcgcgtacga cgtcgcgctg cctcgctcct gacgggggaa
     1621 tagccgcacg ggcgccgttg tttgcgcgcc atggcttcgc cgaaaggaac cgtcatgaag
     1681 cgctcgtgcg tttcgcttct cgtcctcgtc gccgtgctct gcgcttcgtc cgccctcgct
     1741 gcgtttcatt tgtttcgcgt cgaccaggtg tattcgaacg ccgacggcag cgtccaatac
     1801 gtcgtgatgc gcgaggtcac cggctccgac ggcgagaact tctggcaagg caattccctg
     1861 acgacgaccg gcgcgggtgg catgcagcaa ttccagttcc cggcgaatct tccgtcagcc
     1921 tccaccgcga gccgcagcgt gctggtcgcg acgagcggat tcgcggcact cggactcgtg
     1981 acacccgact acacgatacc ggccggcttc gttccgaggg ccggcggcac gctcaattac
     2041 gcgggcgtcg accagatcgc gcttcccgca ttgccggccg acggcgtaac ggcagtcgac
     2101 cgcaacggca accaggttgc ggcaacgccg aagaatttcg ccggcgcgac ggcgacgctg
     2161 acaacgccga cgccgccggc tgcgacggcg cccgacctcg accagcacgg gctcaccggc
     2221 tcgtggttcg aaccggcgac gagcggccag ggcttcgagg tcgagttcta tccggacctg
     2281 gtcgcggccg gcaccgcgct cgtccagggc gcgtggttca cgttcgaggt ggcaccggcc
     2341 ggcggcgccg accggcagcg ctggtacacg ttcaacgggc acgcgcaaag cggcgacgac
     2401 gacgtgaaca tcacgatctt ccagaacgtc ggcggcaatt tcgatgcgcc cccagtcacg
     2461 aatggcgtgg ccgtcggatc gggaacgctg tcgttcgaca cgtgcacgag cgggtcgctg
     2521 aactacgcgt tcagcgacgg cagcgggcgc agcgggacga taccgctgtc acgcatcacg
     2581 ccgaacgtca cgtgcgcaac cggaacggcc gcgccgacaa cgaatgccga cttcgcgctg
     2641 tccggaaact ggttcgaccc cgcgacgtcg ggacaggggt tcgtgttcga agtcaatccg
     2701 atcgtgtcgg tggtgtcgtt tgcctggtac acgtattcgc cgaccggtca gatggcgggt
     2761 gccgcgggcc agcgctggtt cacggggtcg gcgagctacg cgcccggcaa ccgcatgatc
     2821 ccgctgacgc tgttcgagac gacgggcggc gtcttcgatc aaccgacgcc tgcggcacaa
     2881 acgagcacgc ccgttggctc tgcggtggtg aatttcacga gctgctcgac ggcgcaactg
     2941 caattcaact tcaccggcgg cggcaacgcg ggtcacgcgg gcacgatctc cctcgcgcgc
     3001 gtcggtcccg tgccccccgg atgcgtgaac agcagcagcg acgcgtcgat gcagcccggc
     3061 tacggaggcg ggtacgggcc gtagactccg cttgcccggt agtgcggtac gccctgccac
     3121 ccacgacttc aactgccgct gaaccggaag tcgacgtcgg gcttgcggcc cgcgacgacg
     3181 tcggccagcg cgcgcgccga gccgcatgcc atcgtccagc ccagcgtgcc gtggcccgtg
     3241 ttgaggaaca ggttgcgata gcgggtgcgt ccgatcagcg gcgcgttcga cggcgtcgcg
     3301 ggccgcagac ccgcccagta gcgaacgctg tcacgatctc ccgcgtcggg aaagagctcg
     3361 aacgtgcgcg cggtcagggc ttcgcaccgc gtctggttga gcgaggtgtc ccagccgttg
     3421 agttccgcgg tgccggcgac gcgtagcctt tggccgagcc gggtcagcac gatcttccag
     3481 tcgaaatcgg tgagcgaaac cgtcggcgca ccgcgatgcc cgccgatgtc gatcgttgcc
     3541 gagtagcctt tcgccggata gacgttgcag ggcacgtgga cgggcgcgag caggaacggg
     3601 ctgtagctgc cgagcgccat cacgtaggcg tcggcggcaa gggtgtcgaa gcgcgccccc
     3661 tcgcgcacga tgcgcacgcg ctcgatcgca tcgcccgcgg gcgaaagcgc ctggatctcg
     3721 gtgctccatt cgaacgcgac gccttcggcc gccgcgagcc gcgcgagctc cgtcgtgaag
     3781 cgctgtgcat cgcccgattc gtcagtagcc gtgtagatgc cgccggcgac cttgtcgcgg
     3841 cattgcgcga gcgcgggctc gatcgccacg cattcgtcgg gcgtcttcac gtcgcggtcg
     3901 cagccgtaca cgcgcatcag ctcggcagcg tgcacgccgc gatcgaattc ggccgcgtcg
     3961 gtatagaact gcaggatgcc gcgctcgagc tggtcgtagg cgatgccggt gctttcgcgc
     4021 agcaccttca ggcaatcgcg cgaatacagc gcgagattga ggcactggat cgtgttccgg
     4081 cgggtgcgcg acggcaggca ttcgaggaga aaggcgagac cccagcgcca ttgccgccag
     4141 tcgagccgtg gccggaacag cagcggcgcg tcgtcgtgcg cgagccactt caggatcttg
     4201 agcggcgcgc cgggattcgc ccagggctcg gcgtacgaca ccgagatctg cccgccgttg
     4261 gcgaacgacg tctcgagcgc gggcccgggc tgccgatcga cgagcgtgac gtcgtggccg
     4321 tcgcgcgcga gataccaggc ggaagtgaca ccgacgacac ccccgccgag cacgaggatg
     4381 cgcattgggg tcagagaccg atttccggca atggcgcttg cacggatacc accttccgcc
     4441 ggaaatcggt ctctgacccc actttggtct ttccgcgcat gtcgtcaggc aagcgccggc
     4501 gtcagcggaa tgggtgcctg cgcgccggcc tcatcgaccg gcacacagct gcagaacagg
     4561 ttgcggtcac catggacgtt gtcgacgcgc gcaaccgggc accagtactt gtcgccggcg
     4621 agcgtggcaa gcggccacgc cgcctgttgt cgcgtgtagg cgtgcgtcca tgtgtcggcg
     4681 ctgaccgcgg cggccgtatg cggcgcgtgc ttcagcggat tgtcggcgcg gtcgaacgcg
     4741 ccgttctcga ttgcacgcac ctcgttgcgg atggcgatca tcgcggcgac gaagcgatcg
     4801 agctcttcct tcggctcgct ttccgtcggc tcgaccatca gcgtgccagc cactggaaag
     4861 ctcatcgtcg gcgcgtgaaa cccgtagtcg atcagccgct ttgcgacgtc ctcgacctcg
     4921 acgcccgcca gcggcttcag cggccgcagg tcgaggatgc attcgtgcgc gacgaggccg
     4981 tcgggaccgc tgtagagcac cgggtaatgc ggcgcgaggc gcttcgcgac gtagttcgcg
     5041 gcgaggatcg cgctctcggt tgcatcgcgc aggccgcgcg cacccatcat cgtgatgtac
     5101 atccacgaga tcggcaggat cgacgccgag ccgtagggcg cagcgctgac ggcgttggtg
     5161 gcaaccgacc cgtcacccgc ctcgtggtcg ctcggctccc tcgccccggc ggggagaggg
     5221 tgggggtgcg ggggtgcgac gcgcaaatac cgatgccccg gcaagaacgg cgcgaggtgc
     5281 gcccggcatg cgacgggccc cacacccggg ccgccgccgc catgcgggat gcagaatgtc
     5341 ttgtgcaggt tgagatgcga aacgtcggcg ccgaacaggc ccggcgcggc aaggccgacg
     5401 agcgcattca ggttggcgcc gtcgacatac acctgcccgc catgcgcgtg cacgatgtcg
     5461 cacacgtgcc cgatgccggg ctcgaacacg ccgtgcgtcg acgggtacgt gaccatgatc
     5521 gccgcgagcc tgtcgcgatg ctcgcgcgcc ttcgcctcga gctcggcgac gtcgacattg
     5581 ccgtcgcgat cgcagccgac gacgaccacc ctcatgccgg cgagctgcgc cgacgcggga
     5641 ttcgtgccat gcgccgatgc cgggatcagg cagacgtcgc ggtgggcttc gccgcggctc
     5701 gcgtggtacg cgcggatcat cagcaggccc gcgtattcgc cttgcgagcc cgcgttcggc
     5761 tgcagcgaaa ccgcgtcgta tccggtaacc gcgcacagca tgtgctcgag gtcgtcgacg
     5821 agctcgcgat accccgtcac ctggtcggcg ggcgcgaacg ggtgcagtgc gccgaactcg
     5881 ggccacgcga ccggcagcat ctcggacgtc gcgttgagct tcatcgtgca cgagccgagc
     5941 gggatcatcg tgcggtcgag tgccagatcc ttgtcggcga ggcggcgcag gtagcgcagc
     6001 atctgcgttt cggaccgatg ccggtgaaat accggatgcg taaggaacgg cgacgctcgc
     6061 gcgatttccg gcggatacgc atcgtcgaca tcggcgtcga gcgtttcgac gtcgagtgca
     6121 acgtcaccac cggtgaaaat gcgccacagc gccgcgacat cgtgacgcgt cgtcgtctcg
     6181 tcgagcgaaa tgccgagcga ggcaccatcg cgaagccgca ggttgcagcc cgccgccagc
     6241 gcgcgcgcgg cgatcgcccc ggtgcgctcg ccggtgtcga tgaccagcgt gtcgaaagcg
     6301 atcccggttg cgacgtcgaa gccgagtcgt gcgaggccgg cgcgcaggat cgccgtgagg
     6361 cggcgcgttc gccgtgcgat cgccgtcagg ccctcggcgc cgtgatacgc cgcgtacagg
     6421 ctcgcgatga tcgccagcaa cacctgcgcg gtgcagatat tcgacgtggc cttttcgcga
     6481 cgaatgtgct gctcgcgcgt ctgcagcgcg agccggtacg ccggtgcccc cgccgcatcg
     6541 acgctgacgc cgacgaggcg tcccggcaac gcacgcttgt acgcgtcgcg cgtggccatg
     6601 taggcggcgt gcgggccgcc gtatcccatc ggcacgccga agcgctgcgt cgagccgacc
     6661 gcgacgtcgg cgccccattc tccgggcggc gacagcagcg tcaatgcgag cagatcggcg
     6721 gcggcgacga cgagcgtgcc gctggcgtgc agtgcgtcga ccagcgcgcg gtaatcgcga
     6781 acgtcgccat cggcaccggg atactgcagc agcgcggcga acgcatcggc gccggccgcc
     6841 tcgctcaccg ggcccacgac gacttcgatc gcgagcggtg ctgcgcgcgt gcgcacgacg
     6901 tcgatcgtct gcgcgaacac gtcgtcggcc acgaagaacg cgcggcgctt cgacgtgctc
     6961 acccgctggc acagcgtcat cgcctcggcg gccgccgtcg cctcgtcgag catcgatgca
     7021 ttcgcgatcg cgagaccggt gaggtcggtg accatcgtct ggaaattcac gagcgcctcg
     7081 agccgcccct gcgagatctc cggctgatag ggcgtgtacg ccgtgtacca cgcaggattg
     7141 tcgaacacgt tgcgctgaat gacgcccggc atgtgcgtgc cgtaatagcc ctggccgatg
     7201 aacgacttca gcacccggtt gcgcgaagcg atccggcgca gcttcgcgag cgcctccgcc
     7261 tcgccgagcg gcggcggcag ggccagcggt tccgtggacc ggattcccga gggaacgatc
     7321 gcactcatca gcgcggcgcg cgatgcgaag ccgatctcgc gcagcatcgc gtcctgcgcc
     7381 gaccgcggcg tgccgatgtg gcgtgcgaga aactcgtcgt gccgctcgag tgattgcagc
     7441 gcgggcacgc tggtcgcgtc cgtcgtctcg ttgcgttgcg cagcttcagg catcggcgat
     7501 caggcgttta tacgcatcgg cgtccatgaa tgccgacagc gcggaagcat cggccgggcg
     7561 cagcctgaac agccacgcgg catacgcatc ctcgttgacc gcttcggggg agcccgaaag
     7621 cgacgtgttg ctcgcgacaa tctcgccggc gatcggcgcg tacacgtcgc tcgccgcctt
     7681 caccgactcg accaccgcgc aggcatcgcc ggcggcaacc gttctgcccg ccgcgggcag
     7741 gtcgacgtag acgagatcgc caagcgccgc ctgtgcatga tcggtgatgc ctatcgtcac
     7801 cgtgccgtcg tcttcggccc gcaaccactc gtgcgtgtcg gcatatttga gcccatccgg
     7861 gacattcatc gagcgttcct ccagggaatt tttgcaacga cgtcaggcga cgagcacgcg
     7921 tccccgacgc acgaacggcg gcttcaccac gcgcgcaccg agcagacggc cccgtacatc
     7981 gacgcgcacg ccatcgccgt cggcaacggc cgccggaagc cgcgcgagcg cgatcgactt
     8041 tttcatcgtc ggcgaaaacg tgccgctggt gatttcgccg gtaccgtgcg tggtgtgcac
     8101 gctctgatgc gcgcgcagca cgccgcccgc gtcctgcagc acgaggcccg cgaggcggcg
     8161 cgagacgggc ctcgcgagca gtgcggcgcg gccgacgaag tcgcgctcgc ctttcatgtc
     8221 gacggtccag ccgagaccgg actcgagcgg cgacaccgac tcgtccatgt cctgtccgta
     8281 gagattcatg cccgcctcga gacgcagcgt gtcgcgcgcg ccgagtccgc aaggcgtcac
     8341 accggcatcg cgcagcgcct gccaaagcgt agccgcaccg gcggcagcga cgacgatctc
     8401 gaagccatcc tcgccggtgt aaccggttcg tgcaatgaag agttcacccg cgggtgttgc
     8461 ggtcgacgcc gcattgaacg gcgcgagatc cttcgtcgca gcctcgctgc ccggtacggc
     8521 ccgccacacg ttgtcgcgcg cgttcggacc ctggaccgcg agcatcgcga gatcgtcgcg
     8581 cggcacgatg cgaagaccag gcgcccgctc acccgcgagc gcccgcagcc atgcaacgtc
     8641 cttgccggcg gtcgccgcgt tgacgacgag gcggaaccag tcgtcgcgca ggtagtagac
     8701 gatcaggtcg tcgagcacgc ccgcgtcctc gcgcagcagg cacgaataga gcgccctgcc
     8761 tggcttggcg agcttgccga cgtcgttcgc gagcgcgtgc cgcaggaacg tgcgcggcgc
     8821 atcgccgccg ttgccatgga gatcgacgac gcgcatgtgc gacacgtcga acatgccggc
     8881 gccggtgcgc accgcgtgat gctcgtcgat ctgcgagccg taatgcagcg gcatgtcgaa
     8941 gccgccgaag tcgaccatgc gtgcgcccag cgcgcgatgc acggcgttga gggcggtggt
     9001 gcttgtcaca tgcgcttcct ggcgtgaacg aacgaagggc ggcgcggatg tccgcgccgc
     9061 cccgtctgtc cttttacctg agagattgcg gcgaacggtg ttgcgttgcg ccggtgcccc
     9121 ttcggtgggt tgcgtcaacc gccacgcaac cgctctccag atcgcgcctg cctttgcaca
     9181 cagccctttt gcctgagcgg tttcgggatt acgccttcgg cggcgtcgtc gacgtcagac
     9241 gctctctcct gcgtgcgcgg caagcgtagc acaaacatgc gcgcgcggcg cgattgccgc
     9301 agtgcatcaa aacgcgctgg caataccgcg tgtgctactt cttgagcgcc aggatcatct
     9361 cgaccatctt cttgatgtcg gcgtcggaca cctgcgcatt cggcggcatc ggcaccgggc
     9421 cccagacgcc ggagccgccc tttttcactt tctcggtgag cttggcgacc gcggtcgagt
     9481 cacccttgta tttggcggcg acgtccttgt acgccggccc gaggatcttc ttgtcttccg
     9541 cgtggcaggc gttgcaaccg ttctttttca tcatgtcctc gagctggtcc gccatcgcgg
     9601 cagacgcgaa aacgagggtg gcggcggcga agacggcggt accgattctt ttcatcggag
     9661 ctccttgtgc attggaaatg ggaacgaagc gcgtgcgggt gaccgagcgc taaaaataac
     9721 atgcgtcagc ttgcatccag cgcaaggatg attgcatcga cggccgcaac gggcggcagg
     9781 cactgcaggc cgcggcagat ccaggcacgc gcaccgtctg ccggcgcatc gcccttgcgc
     9841 agcggcgccg gtgcgcccgc acccgcgatg tcgacgatgc agacgtccgg ccgatacttg
     9901 cgctcgagca tccgttgcca cgcctgcgtg gttgcacgct cgccgtcgag cacgacggtg
     9961 gaaggcggtg cgagcaggcg ctcgagcgcc accagcagcg tggattgcgc accgcccgat
    10021 tcggcgagcg ccggcgcgaa gagcttcaat gtccgctccg ccgcgtcgac gtatcggggc
    10081 tcggcggcca ggtgaccgag catgatcagc gactgtgccg cgatcccgtt gcccgagggc
    10141 gtcgcattgt cgtgcagggg cttcgtgcgg tgatagagcc gctcgtggtc atggctcgtg
    10201 aaccagaacc cgccgcgctc gcggtcctcg aagcgctcga gcagcgcatc ggcgagctcg
    10261 acggcgaacg agaagtcgcg caggcgaaat ctcgtctgca tggcctcgag caacgcggca
    10321 agcaggaacg catggtcgtc gagatacgcg ggcaacgacg cgacgtcgcg gtggcgtgtc
    10381 gcgtaaaggc gaccatcgcg ccaggccgtt gcgaccagcg tatcgagtgc tgcgaacgcg
    10441 agatcggcga acgcgggctc gcaaagcgtc cgcgcggcgc gcgccagggc ggcgatcatc
    10501 agcgcattcc aggacgtgag caccttgtcg tcgagacccg ggcgcacgcg cgccgcccgc
    10561 gccgcgagga gagcggcacg cgcacgcgag agccgcgctt cgacatcggc cggcgatgcg
    10621 ccgagcttcg ccgcgacgtc gccgatcgac gccgccacgc gcaggttgaa ggcgtgctgc
    10681 tcgaaattag gcggcccgtc gagcccgtaa tgcgccgccg caactgtcca ttcgtcgccg
    10741 gacagcacgg cgcgcacctc gtcgcgggtc cacacgtaat acttcccttc gacaccctcg
    10801 ctgtcggcat cgaggctcga gtagaacgcg ccgtcgggtg cgcgcatttc gcgcacgagc
    10861 cagcttacga tcgccttcgc gacagccccg aagcgcggct cgtcgagcgc gcgcgccgcg
    10921 tcggcgtaaa gcgcgagcag cgcagcattg tcgtagagca tcttctcgaa atgcggaatc
    10981 gaccagcggg cgtcgacgct gtagcggcag aatccgccgc cgagctggtc gaagattcca
    11041 ccggccgcca tgcgctcgag cgacgtgcgc acgatcgtgc gcgcgccatc gtcgccgcga
    11101 agcctccatg cgcgcagaca gaagtcgagc tcggccgcat gtggaaactt cggtgcgccg
    11161 ccgaatccgc cgtcgaccgg gtcgaacgcc tgcgtcaggc gcgcgagttc ctgcgcgggt
    11221 gcgcccgacg gaagcgcgtt tgcgggatga gtcggctcca gcgcatccag cgcccgcgaa
    11281 agccgttcgg cctgctcggc gattgccggg ccctggtcgt gaaacgcatc ggcgatacgc
    11341 ggcaggagat cgagaaatcc gggcatcccg tagcgcggct gcttcggaaa ataggtcccg
    11401 gcgaaataag gcgcaccgtc gggggtgaga aacaccgtga gcggccagcc gcccgagcgt
    11461 cgcgtcatca gcgcgtgcgc ggtctgatag atctggtcga gatcggggcg ctcctcgcga
    11521 tcgtccttga tgttgatgaa gcgctcgttc atcaccgcgg cgaccgccgg gtcctcgaac
    11581 gactcgtgcg ccatcacgtg gcaccagtgg caagcggagt agccgatcga cagcaggatc
    11641 ggcttgttct cgtccctggc taaagtgagc gcttcctcac cccagggaag ccacgcgacc
    11701 gggttgttcg cgtgctggcg caggtaaggg ctgctggccc cggcgagacg gttggccatc
    11761 ccgcattatc gcgcgccgaa cggaacggcg atcgggccgg ctgaccccgt accgtttgcg
    11821 ggtcgagggt cggctcgccc ggagctagaa atcggggtcg accgcctcga acgcgacgcg
    11881 ctcgaaatcg ccgccgcgct gcaggatgtg cgtcagcctg aatttgcgca tctgtcccgg
    11941 ctcgtcgcaa cgcatgatcc ggttggggat gaacgcccgc ggcgacatca ccagcgtcgt
    12001 cggctcctcg ggcgtctcgt cgaggatcag cgcagcaacg ggcggttcct cggttcgatt
    12061 cggattgacg aggcgcaccg cgaacacctt cggcgcaccg gcccagagcc tgattgcaat
    12121 ggtgatcgcg ccgtcggcgt cgaccgcgac gcgcgaaaca tggccgagac gcgtttcgcc
    12181 ctcctcgcgc acgacgacga gctggtcgag gaaccagtga cagccactcg caccctggtg
    12241 gcgcagcgac gcctcgcgcc attcgcagct cccgtaccac ttctcccagg gccagcttcg
    12301 ttcggcctcg tccttgcgcc ggtcgaaatc ggaaataccc ccgacggtct gcaggtattt
    12361 cgtcggatcg ctgtcgcgac ccagcgggtc cacgtggcgg aacgaacgtc cgccgacgcg
    12421 gaaatacgcg ccttcgatgc cgcccgcggc gatctcgacc gctttcgagc gcttgcgatt
    12481 ggcgccgccg ccgacccgat gccattgggc gtcgaggtgc gccaacaggg cgctggccgc
    12541 gtcggccgac acgtcctcgc caagctgcag gtcggcggga gaggccccgc tcgcgagacg
    12601 cttgagacgt ccgcgaacgc tcgtcgaaag cttggccgga tagccgaagc gccaggatgg
    12661 cccttgtgcg ctcgccgtcg tggcggccag catcgcgccg tgcgcaccct cgaggtcgac
    12721 gacaatgatc ggcccttcgg tctcgcgctg cttcgcgtac ggaaagagct tgcgcgccca
    12781 tagtccgagc cagcggtcgg tgagctcgat ctggcgcgcg ctcagcgcat agggatcggc
    12841 cagcgcgagc aacaacgtgt ggctgtacgt cgaatagcac gacaggccct cggcgccggg
    12901 catcaggtga tcggagaccg aagcggtggt gcagtcgagc atttcggcga gccggtagta
    12961 cgcatgcagt tcctgccaaa gctcggcatc gggcacgcgg cgcgcaagcc cgtgcacgac
    13021 gatgagctgc ttgcccacgt agagcgcgcg ctgcaggagc ttcgccttga cccccttgag
    13081 ctcggtgctg ccttcgagca gcgggcgcag gcacaccgag tactggatcc agagcgcctg
    13141 ccatagcgcg accgcctggt cggcggcatc ggcttcccgc tcgtcggacg gctgcggccg
    13201 gcccgcgtag cggcgtgcaa gctcggtatg caggtgcgca accggctcgc gtgcgagctc
    13261 ggcgatggtc gcgcgatcgc gcgggcccat ctccgacgcc gtgagcgcgc gcagctggcc
    13321 gagcagcatc tcgtaggcct gcccgaccgc ggacaacggc agcgccttca tccatcggcc
    13381 ggcactcgcc gcgtcgttga aggcgagctc cggcaacgat tcgggcccgg ggtccgcctc
    13441 acccgtggca tccgcgtcga cgagcggcgg agcggcgacg tcctgcatgg tgattctccg
    13501 aaagacggta cgatcgcacc ggcgtggtcg tcgagcaaac aacccaaggc acgcgcttac
    13561 gcgacgccgg gggatcgaca acggctaccg gtcaagtttc atgcttatag catagccgtc
    13621 ggccggcatc gatgcgcgcg cggagaccag ccgatgagcg gcgcggagac gttaattttc
    13681 cgcatcgcgg acgcgatttt tacgctgcct ccgggacgcg cggcgcctag gatgaccacg
    13741 acggtcccgc gatcgtgcga ggccgcgaaa ggcaaccctt tcctccaccc gcggcgatgc
    13801 gttcgacacg ccggccggat tcgcgacgga cgctttccgc gggatctttt tcaggagggc
    13861 cggggagcga tcgccgcgtt tcccggtgga ctcgggtttg taccagggcc cgttaacggg
    13921 ccctagacca ggacccgctc gatgccgccg cggttcgcct tcgcgacgta gtcggccatc
    13981 cacgattcgc caagcacgtg cttcgcgagc tcgacgacga tgtagtcggc gtcggtgccc
    14041 gcgtcgtcct cgaagcgcga cagtccctgc aggcacgacg ggcacgatgt cagaaccttc
    14101 acctgccccg tgaagccgtc ggcgcgtaac gcgtcggcgc ccgcgcgcat ttcgtcttcc
    14161 ttgcgcagcc gcacctgcgt cgagatgtcg ggccgcgtcg tggcgagcgt ccccgattcg
    14221 ccgcagcagc gatcgttgag cggcacgtcg gtgcccatga gctggctcac caccgcgagc
    14281 ggcttgtaga ccttcatcgg cgtgtggcag gggtcgtggt acatgtagcg cacgccctcg
    14341 acgccctcga gcgcgacgcc cttctcgagc aggtactcgt ggatgtcgag cagccggcag
    14401 cccgggaaaa tcttgtcgaa ctcgtacttc tgcagctggt cgaggcacgt gccgcaggag
    14461 acgatcaccg tcctgatgtc gagatagttg agcgtgttcg cgacgcggtg gaacagcacg
    14521 cgattgtcgg tgatgatctc ctggcccttg tcgtggttgc ccgccgccgt ctgcggataa
    14581 ccgcagcaca ggtagcccgg cggcagcacg cactgcgcgc ccacgtgata cagcatcgcc
    14641 tgcgtcgcaa gcccgacctg cgagaacagt cgctcggagc cgcaaccggg gaaatagaac
    14701 accgcgtcgg aatcgtcgtt gaccttcgac gggtcgcgga tgatcgggat gatcgtcggg
    14761 tcctcgacgt cgagcagcgc gcgtgcggtg cgcttgggca cgcccttcgg catcggcttg
    14821 ttgacgaagt ggatcacctg cgccttgacc ggcgtgcggc cggtcgtcga cggcggatgc
    14881 gcggtctgcg cacgcgcgat cccggcgttc ttcacgatcg aatacgcgag gcgctgcgcg
    14941 cgatagccga agtcgatcat cgccatcttc gcgagcttga tcgtcgccgg atccgtcgca
    15001 ttcaggaaga acatcgccgc cgcggtgccg acgttgaact tcttcttccc ttcgcggcgc
    15061 agcaggttgc gcattgcgat cgacacgtcg ccgaagtcga tgtcgaccgg gcacggattg
    15121 aggcatttgt ggcagatcgt gcagtggtcc gccacgtcgc cgaactcgtc gaaatggcgc
    15181 agcgacacgc cgcggcgcgt ctgctcctcg tagaggaacg cctcggtcag aagcgacgtc
    15241 gcgaggatct tgttgcgcgg gctgtagagc aggttcgcgc gcggcgaatg cgtcgcgcac
    15301 ggcgccttgc acttgccgca gcgcaggcaa tccttgatcg cggtcgtgat ttcgccgatc
    15361 gcgctctgct cgaggatcag gctctcgtgt ccgatcagcg agaacgacgg cgtgtacgcg
    15421 ttcgtgagat ccgacggcag cccgcgcgag cgcacgagct tgccgcggtt gaagcggccc
    15481 tcgggatcga tgcggcgctt gtagtcggcg aacggcgcga gctcgtcgtc ggtaaggaac
    15541 gcaaccttcg tgatgccgat gccgtgctcg ccggagatga cgccgttcag cgcgcgcgcc
    15601 agcgccatca cgcgctcgac cgccgcgttc gcctgctgca gcatcccgta gtcgtccgag
    15661 ttgaccggga tgttcgtgtg tacgttgccg tcgccggcat gcatgtgcag cgcgatgaac
    15721 acgcggcttc gcagcacctc gcgatggatc tcgtcgcagc gcgcgaaaag cggcgcgaac
    15781 gcgcgccccg cgaagatccg ctcgagcggg ctccgcagct cgcgcttcca cgagacgacg
    15841 accgcgtggc tttgcaggtc cgcaaacgtc tcgtcgagac gatcgagcag gtactgccac
    15901 tgcgcgcgta cctcgctcac gagcgcgcgc gcctcgtcga gcgcggcggc gagctcggtg
    15961 tccgtgggac gcgcctcgct gccgggacgc cacaggtgct cgacgtcggg ctcggcaaag
    16021 aattgcgtca gacgatcggc gagcacgagc ttgttgccga tcgacatcgc gatgttgagc
    16081 cgctcgacgc cgtcggtgta gtcgccgagc cggtcgagcg gaatgacgac gtcctcgttg
    16141 atcttgaacg cgttcgtgtg cttcgcgatc gcggccgtgc gcgagcggtc gagccagaac
    16201 ttcttgcgcg cctcggcgga caccgcgacg aagccttcgc caccgcgcgc gttggcgatg
    16261 cggatcacat cggatgcggc cttcgcgacg gcctcgtcgc gctcgccgac gatgtcgccg
    16321 atcagcacca tcttcgggta gccgtgacgc ttcgctttcg tcgcgtagcc gaccgccttg
    16381 acgtagcgcg cgtcgaggtg ctcgagcccg gcgagttgca cgcgttcgcc gtccggcctt
    16441 cgtgccgtgt cgtcgaggaa gcgcttgatc tcgacgatgg ccggcgtcga ttcgcgtgcc
    16501 tcgccgaaga actcgaggca gaccgtacgg atcgacggcg gcatccggtg cagcacgaag
    16561 cgggcgctga cgacgatgcc gtcgcagcct tccttctgca cgcccggaag gccggccagg
    16621 aacttgtcgg tgacgtcctt gccgagtccc gccctgcgaa agcggtcacc gggtatcgtc
    16681 agcgtttcac ggcg
//
LOCUS       DASSVX010000059         4190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1452060, whole genome shotgun sequence.
ACCESSION   DASSVX010000059 DASSVX010000000
VERSION     DASSVX010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452060"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="VFO53_02730"
     CDS             <1..1481
                     /locus_tag="VFO53_02730"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET8875218.1"
                     /translation="RGALDAVERELQEQQSAWHRDSLALASQQRRCHDLELELVQLRQ
                     AAEAASRRRQQIAQESDELAGQHAQGSEELTVMGATIADMQSRLHDAFAQRDAARNVR
                     NEAEVALARGREQLRVVERAAQEAGYAQRSSRDRLAEIARRREGLAAQVTQQQALLNQ
                     LTSERASIDWTPVEEALQRQLGLRGAAEQALADARVKQETLTAQLRSSDEARLSAEQK
                     LEPARAKIQEMQLKEQAAQLAEQQFIEQLADAKADVATLPDALKAWGSARTLPAEIER
                     VSAAIAELGAVNLAALDELAQASERKTYLDRQADDLTEAMSTLEAAIRQIDRESRELL
                     QQTFDIVNENFAKLFPTLFGGGQAKLVLTGEEILDSGVQVVAHPPGKRNTTIHQLSGG
                     EKALTAISLVFALFQLNPAPFCLLDEVDAPLDESNTHRFCKMVQDMAAESQFLFISHN
                     KITMEMANQLVGITMPDPGISRVVAVDITEALELASETELAT"
     gene            1561..2751
                     /locus_tag="VFO53_02735"
     CDS             1561..2751
                     /locus_tag="VFO53_02735"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HET8875219.1"
                     /translation="MSSLQLVLIIAGVLLVVGVIVYNQWQERRLKGRTAPSAPPEPRS
                     SRVAERVEPTLSTTAPLPAAEGTPAFRGDTTVSEFEAPVDVIAPPIETAATLVDETPA
                     SMAAATSAAPLAPSKGQAARASSMPDHEIECLIPLQPAAPVTVAALGAGLGARIGKPV
                     RWFGRVDARSDWQRLDAETQGAFAEIVACLLLADRNGAASRAQLDTFIRVVGDVAHTL
                     PAAFTPPEVAQELARAEALDRLCAELDVQIGLTVQKPDPGSIAGTRLRGVAEAAGFRL
                     APGGRFEYAHEDTGAVLYTLQNLRPDPFTAESLRLTATNGVVFLLDVARTADPVRTFD
                     QMKLAAKRLAKTLGAELVDDNHRPLDDAAFTVIREQVDGAVEALRAVHIEPGSARALA
                     LFSA"
     gene            2851..>4190
                     /gene="ligA"
                     /locus_tag="VFO53_02740"
     CDS             2851..>4190
                     /gene="ligA"
                     /locus_tag="VFO53_02740"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET8875220.1"
                     /translation="MPAAARARVDALREAITAHNHAYYVLDEPTVSDAEYDALYRELE
                     ALEREHPALVSTDSPTQRVGGAALTAFAPVRHAVPMLSIRTETNTTAGGAANFDARIR
                     RDLGLSDTAAPVEYIAELKFDGLAVSLRYDAGRLAIASTRGDGEVGEDVTRNVHTIRS
                     VPLKLSTRKPPGVLDVRGEVYMSRKDFALLNARQEAAGLRTFINPRNTAAGAVRQLDP
                     AMTAQRPLRFFAYGIGEVSDWELPPTHAAMLDALAAFGLPVDPHRRVVHGAEGLVAFY
                     EHVKTLRDKLPFDIDGVVYKVNDLALQRRLGFVTREPRWAVAHKFPAEEMPTELLDIG
                     VQVGRTGAITPIARLRSVFVGGVTVTSATLHNEDEVRRKDVRIGDTVIVRRAGDVIPE
                     IVRVLTERRPADAREFVMPAACPECGSAIVRLPDEAVARCTGGLICPAQRKQALR"
BASE COUNT          749 a         1398 c         1405 g          638 t
ORIGIN      
        1 cgcgaggtgc gctcgatgcc gtcgagcgcg agctgcagga gcagcaatcg gcatggcacc
       61 gcgacagtct ggcgctcgcg tcgcagcagc ggcgatgcca tgacctcgag ctcgagctcg
      121 tgcaactgcg acaggcggcc gaagcggcga gtcggcgtcg acagcagatc gcgcaggaga
      181 gtgacgagct cgccggccag cacgcgcagg gcagcgaaga gttgacggta atgggcgcga
      241 cgatcgcgga catgcaatcg cgattgcacg acgcgttcgc gcaacgcgat gcggcacgca
      301 acgttcgtaa tgaagccgag gtggcactcg cgcgtggacg cgagcagctc cgcgtggtcg
      361 agcgcgcggc gcaggaagcg ggctatgcgc agcgaagctc gcgcgatcgc ctcgccgaaa
      421 ttgcgcgtcg ccgcgaagga ctcgcggcgc aagtgacgca gcaacaggcg ctgctcaacc
      481 aactgacgag cgagcgcgca tcgatcgact ggactccggt cgaggaagcg ctgcagcggc
      541 aactcggtct ccggggggcg gccgagcagg cgctcgccga tgcgcgcgtg aagcaggaaa
      601 cgctgacggc acagcttcgc tcgagcgacg aagcacgcct ttcagccgag cagaagctcg
      661 agcctgcgcg cgcgaagatc caggaaatgc aattgaagga gcaggccgcg cagcttgccg
      721 agcagcagtt catcgagcag cttgccgatg cgaaagccga cgtcgcgaca cttcctgatg
      781 cgctgaaagc gtggggcagc gcgcgaacgc tccctgccga gatcgagcgc gtgagcgccg
      841 cgatcgccga gctcggcgcc gtcaacctcg ctgcgctcga cgagcttgcg caggcgagcg
      901 aacgcaagac gtatctcgac cggcaggccg acgacttgac cgaggcgatg agcacactcg
      961 aagcggcgat ccgccagatc gaccgcgagt cgcgagagct gctgcagcag acgttcgaca
     1021 tcgtcaacga gaatttcgcg aagctctttc cgacgctgtt cggcggcggt caggccaagc
     1081 tcgtcctgac cggcgaggag atactcgatt cgggcgtgca agtcgttgcg catccgccgg
     1141 gcaagcgcaa cacgacgatc catcagctat caggtggcga aaaagcgctg acggcgatct
     1201 cgctggtgtt cgcgctgttt cagctcaatc cggcgccctt ctgcctgctc gacgaggtcg
     1261 acgcgccact cgacgagtcg aacacgcatc gcttctgcaa gatggtgcaa gacatggccg
     1321 ccgaatcgca gttcctcttc atctcgcaca acaagatcac gatggaaatg gcgaatcagc
     1381 tcgtcggcat cacgatgccc gacccgggga tctcgcgggt cgtcgccgtc gacatcaccg
     1441 aggcgctcga gctcgcatcc gaaacggagc tcgcgacata aacgcgcacc gcgtaagcgt
     1501 gacgctatac tcgatcatgc cgagctcgat cgacgcgcac gcatttccgg cgaggaccgt
     1561 ttgagctcgc tgcagcttgt tctcatcatc gccggcgtac tgctcgttgt cggcgtcatc
     1621 gtctacaacc agtggcagga gcgacggctg aaaggccgca cggcaccgag cgcaccgccc
     1681 gagccacgct catcgcgcgt tgccgagcgc gtcgagccga cgctgtcgac gacggcgccg
     1741 ctgcctgcgg cggagggcac gccagcattt cgtggcgaca caaccgtaag cgagttcgag
     1801 gcgcccgtcg acgtgatcgc gccgccgatc gaaactgctg cgacgctcgt cgacgaaacg
     1861 cccgcgtcaa tggcggccgc gacgtccgca gcgccgctcg cgccatcgaa aggccaggcc
     1921 gcgcgtgcgt cgtcgatgcc cgaccacgag atcgaatgcc tgattccgct gcagccggcc
     1981 gcacccgtca ctgttgccgc actcggcgcc ggactcggcg cgcgaatcgg caagcccgtg
     2041 cgctggttcg ggcgcgtcga tgcgcggtcc gactggcaac ggctcgacgc cgaaacacag
     2101 ggagcgttcg ccgagatcgt cgcgtgcctg ttgctcgccg atcgcaacgg cgcggcctcg
     2161 cgggcacagc tcgacacgtt catccgcgtc gtgggcgacg ttgcgcacac gctgcccgcc
     2221 gccttcacgc cccccgaggt tgcacaggag ctcgcgcgtg cggaagcgct cgatcgtttg
     2281 tgcgccgagc tcgacgtgca gatcggtttg acggtgcaaa agcccgatcc cggctcgatt
     2341 gccggaacgc ggctgcgcgg cgttgccgag gcagccggct tccggctagc acccggcgga
     2401 cggttcgagt acgcgcacga ggacacgggt gccgtgctct acacgctgca gaatctgcgg
     2461 cccgatccgt tcaccgccga gtcactgcga cttacagcca ccaacggtgt tgtctttctg
     2521 ctcgacgtcg cgcgaacggc cgatccggtt cgaacgttcg atcagatgaa gctcgcggca
     2581 aagcgccttg cgaagacgct cggcgccgag ctcgtcgacg acaatcaccg gccgctcgac
     2641 gacgccgcgt tcacggtgat tcgcgagcag gtcgatggcg ccgtcgaagc gctgcgtgcc
     2701 gtgcacatcg aacctggcag cgcgcgtgcg ctggcgctgt tcagcgcgta gggccggcta
     2761 tctggcccta gttcatttgc ggcagcactc atcggcagcc cggagcgccg tggcagctct
     2821 cgcgtgagca acgtcaaagc ggattccaag attcccgcag cggcgcgggc gcgtgtcgat
     2881 gcgttgcgcg aggccatcac cgcgcacaat cacgcttact atgtgctaga tgagcccacg
     2941 gtaagcgacg ccgaatacga cgcgctttac cgtgagctcg aagcgctcga gcgcgagcat
     3001 cccgcgctcg tcagcaccga ttcgcccacc cagcgcgtcg gcggcgccgc gctgacggcg
     3061 ttcgcgccgg tccgccatgc agtgccgatg ctgtcgattc gcaccgagac gaatacgacg
     3121 gcgggtggcg ccgcgaattt cgacgcgcgc atccgacgcg atctcggcct ttccgacacg
     3181 gcagcgccag tcgaatacat cgccgagctg aaattcgacg ggctcgctgt cagtcttcgc
     3241 tacgacgcag ggcgtctcgc catcgcatca acgcgcggcg atggcgaagt gggcgaggac
     3301 gtcacgcgca acgtccatac gatccgctcg gtgccgctca agctttcgac gcggaagccg
     3361 cccggtgtac tcgacgtgcg cggcgaggtg tacatgtcgc gcaaggactt cgccctgctc
     3421 aacgcgcgcc aggaagcggc gggtctgcgc acgttcatca atccacgcaa cacggcagcg
     3481 ggcgccgtgc gccagctcga cccggcaatg acggcgcaac ggccgttgcg gtttttcgcg
     3541 tatggcatcg gcgaagttag cgattgggag ttgccaccga cacatgcggc aatgctcgat
     3601 gcgctcgccg ccttcggctt gccggtcgat ccgcatcgcc gcgtggtcca cggcgcggaa
     3661 gggctcgtcg cgttctacga gcacgtgaag acgctgcgcg acaagctgcc gttcgacatc
     3721 gacggcgtcg tctacaaggt caacgacctc gcgctgcagc gcaggctcgg cttcgtcacg
     3781 cgcgagccgc ggtgggccgt cgcacacaaa ttcccggccg aggaaatgcc gaccgagttg
     3841 ctggacatcg gggtgcaagt cgggcgcacg ggcgccatca caccgatcgc tcggctgcgc
     3901 agcgtgttcg tcggcggcgt caccgtgacg agcgcgacgc tgcacaacga ggacgaagtg
     3961 cggcgcaagg acgtgcgaat cggtgacacg gtgatcgtgc gccgcgcagg cgatgtcatt
     4021 ccggagatcg tccgcgtgct gaccgagcga cggccggcag acgcgcgcga attcgtgatg
     4081 ccggcggcgt gtcccgaatg cggttcggcg attgtccgct tgcccgacga ggcggtcgcg
     4141 cgctgcacgg gcgggctcat ctgcccggcg caacgcaagc aggcgctgcg
//
LOCUS       DASSVX010000060        16964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1535229, whole genome shotgun sequence.
ACCESSION   DASSVX010000060 DASSVX010000000
VERSION     DASSVX010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 16964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16964
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535229"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFO53_02745"
     CDS             complement(<1..505)
                     /locus_tag="VFO53_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8875221.1"
                     /translation="MGSPMSRRQLLAAAAGAGLYAMLPFARAQSKSLVVATFPGTWNE
                     AHRNLLAPYFRKRTGAGVTQSIQLATEQIAKLEAAKGGRPPFDVAILDEPQVFDAAKQ
                     GLIDEYPAAKSPNFKELFPVYQDKWGPKISLQVIGIGYNPNKIKTPPKSYDDLLNPAY
                     KGRVGLTA"
     gene            complement(555..1655)
                     /locus_tag="VFO53_02750"
     CDS             complement(555..1655)
                     /locus_tag="VFO53_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875222.1"
                     /translation="MTDAPKGHRLTLERVTHRFGDVTAVEGIELDIAGGELVALLGPS
                     GCGKSTLLRIIAGFIAQSEGRVLFDGEAVDHVAPTRRGAGIVFQNYALFPHMTVAQNV
                     AYGLEARRLAKDRIESRVADMLRLVHMQSYAQRLPRQLSGGQQQRIALARALAVDPKI
                     LLLDEPFGALDKNLRLDMQIEVKRLQREYGITAILVTHDQEEALSMADRIAVMNRGRI
                     EQLSSPTDVYDRPQTVFVNQFVGTTNLLHGELADAVLGVVRLEGGGLMAAAPAPGLRQ
                     GDRVVLSVRPEQLRIHSAHVDGAVTATIRVVLPLGPHVMYEARTDTGVAFRISAPRDA
                     GAQLLLPGECVHVAPVAAHACNMFAAQSERAA"
     gene            complement(1675..2028)
                     /gene="uraH"
                     /locus_tag="VFO53_02755"
     CDS             complement(1675..2028)
                     /gene="uraH"
                     /locus_tag="VFO53_02755"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HET8875223.1"
                     /translation="MGKLTTHVLDTSAGKPAAGMRVDFASLDREAAPGVRTVYTNAEG
                     RTAEPLLPADAMRCGEYELTFHVADYFRGQGHALADPPFLDRVLIRFAIADVAAHYHV
                     PLLCSPWAYSTYRGS"
     gene            2153..3040
                     /locus_tag="VFO53_02760"
     CDS             2153..3040
                     /locus_tag="VFO53_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET8875224.1"
                     /translation="MDLRHLRYFATLAQELNFTRAAQRLNVSQPPLSQQIKALETELG
                     TLLFVRTSRRVELTAAGHVFLAHASAILERVEQACAQARAVGGGHVGHLDIGSSSSLL
                     LGPLPQLIAGYRRTFPGVNIALHEMTPAAQLTALRDRRLDISFSRTPVLDQHLSAELA
                     WEDPVVAALPRAHRLVARRKLALKDLANEEFVLLRLDSSAFAVYLNQCCVDAGFLPRA
                     SQYVVESQAMPSLVAAGLGVALVPASLARVHRQGVEYRTLAGAAPRADVYALSRRDDP
                     SATVRGFLEKAREILAAGK"
     gene            3116..4387
                     /locus_tag="VFO53_02765"
     CDS             3116..4387
                     /locus_tag="VFO53_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET8875225.1"
                     /translation="MATPLPQLGDLDEGAAPFDELNPAPRLLMGPGPINAHPRVLQAL
                     AAPMLGQFDPQFRAYMKQTMSLYRRVFETRNRWTLLVDGTARAGIECVLASAVEPGER
                     VLVLSFGRFGQLMIEIARRAGADVRVVETAWGTVFRPEQVEAALVRHSPKLVAVCQGD
                     TSTTMAQPLDALGALCHRHGAMLQVDATATLGGMPLPVDAWQIDCATSGLQKCLAGPS
                     GSAPITISERMAQTVYRRRHIEEGLRPANYVPGEGAIVGSNYFDLAMLIDYWSDLGLN
                     HHTEATSMLYAARECARLFLQEGHERAFRRHALAGEALAAGVEAMGLRIFGDRAHRMP
                     NVTGVHIPDGVAGDRVRSALLADFNIEIGTSFGPLQGKVWRIGTMGVNARKDAVLTTL
                     GALEAVLAGEHVKLPRGAAVDAARAVYREDE"
     gene            4380..5885
                     /gene="xdhA"
                     /locus_tag="VFO53_02770"
     CDS             4380..5885
                     /gene="xdhA"
                     /locus_tag="VFO53_02770"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HET8875226.1"
                     /translation="MSDAIRFLLNGERVDVAGVAPQTTLLDYLRDERRLTGTKEGCAE
                     GDCGACTVALAERAGDALAWKPVNACIRLLPSVDGKAVFTVESLASDDGAPHPVQQAL
                     VECHASQCGFCTPGFAMSLFGLYKNASRPSRNAVDDALSGNLCRCTGYRPIVDAAMRM
                     YALPPAAGWRGRGVADDGTRIVAPDEARLAADLALLARDDTFDYAHAGRRWSAPRTLA
                     ALADACTARPQAHVVAGMTDVALWVTKQHRDLDDVVYLGDVDDLHAIRETGAGVEIGA
                     AVTLTDALPTLDALFPELHEAWQRFASVPIRNSATLAGNVANGSPIGDSMPALLALDA
                     RVLLRSAAGEREVALDAFYPGYRQTARVPGEFIAAVRIPRRVEGLLLGAYKISKRYDQ
                     DISAVFACFALVLDAGRIRGARIGCGGVAATPVRAHRAEATLVDAMWDRATAEHAAQT
                     LTGEFAPIDDMRASAAYRRTVLGNLLRRFWLESAARPDAPTRVEMLLPQTR"
     gene            5882..8218
                     /gene="xdhB"
                     /locus_tag="VFO53_02775"
     CDS             5882..8218
                     /gene="xdhB"
                     /locus_tag="VFO53_02775"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HET8875227.1"
                     /translation="MNAPRPPWDSATGVVGVPAAHDSAALHVAGEAHYTDDLPEPRGT
                     LYAALGLSPHAHGRIERLDLDAVQGAPGVVAVVTAADFIVNDVGPIEADDPILADRIV
                     QFAGQPVFGVAATSVGAARRAATLARLDVAPLPAILTIDDALAAQSFVLPPVHVTRGD
                     PQEALARAPHRLAGRAASGGQDHFYLEGQVALAVPREHDGMQLFVSTQHPGEVQMLVA
                     RALALRVNDVVVECRRMGGAFGGKETQMSLFACVAALLARRTGRAVKLRLDRDDDMRA
                     TGKRHAFEYRYDAGFDDAGRILALDVTLASRCGFSADLSGPVNDRAAFHADNAYWLPN
                     VAIHSYRCRTNTVSDTAFRGFGGPQGMFAIETVIDDIARTLALDPLDVRKANLYGTTD
                     RNVTPYGMSVEDNIAPVLIDALETRADYRARRRAIAQWNRESPVIKRGMALTPVKFGI
                     SFTATHYNQAGALIHVYRDGSVLLNHGGTEMGQGLFVKVQQVVAHELGVALDSVRVSA
                     SDTSKVPNASPTAASSGSDINGMAARAAARKLRERLAAFAAGESGVAVEHVHFAGGMV
                     VAGSERTTFAALVDRAYHARIPLSATGFYATPKIHYDRRTLSGRPFFYFAYGAAVTEV
                     AIDTLTGEHRLCAVDILHDVGASLNPAIDRGQIEGGFIQGYGWLTMEELCWNARGELT
                     THAPSTYKIPTSRDVPPHFDVVFFGEPNREETIHRSKAVGEPPLMLALSAFHALRDAI
                     ASVADQRLVPRLDAPATPQRVLAAIDDVRARDRATPRA"
     gene            8218..9327
                     /gene="xdhC"
                     /locus_tag="VFO53_02780"
     CDS             8218..9327
                     /gene="xdhC"
                     /locus_tag="VFO53_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HET8875228.1"
                     /translation="MDDANTLPWLDALAATLARDREAVLIVVATTRGSTPRESGAAMV
                     VSRDACAGSIGGGHLEFEATRIARDALCDPAAAGGTWIVRFPLAARLGQCCGGVASLA
                     FARLGANANAWLEPAIACARASTPFAIVTRIGGAPASEGRLVVTSDDVRGSLGNVALD
                     SGAIGLARARLAAASNGAAIVRFARDDDAPLLIQVERCDEFPVLVFGNGHVGRALVSV
                     LGVVPAQVRWIDARADDFARRIPGNVEVVVTDAPEDELADAPHGAYVVVTTHSHALDF
                     ALIEAALARDDWRYVGLIGSKSKRAQFERRLAARGLGDEAFARIRCPIGSEVAIRAKH
                     PGAIAIAIAAELLVVRESGARRAGVPREFALVRKG"
     gene            9452..9811
                     /locus_tag="VFO53_02785"
     CDS             9452..9811
                     /locus_tag="VFO53_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HET8875229.1"
                     /translation="MKEKRWQDWLNLLIGIWLFISPWVIGFVGTDASASWNAWILGVA
                     IVVFSAIAVSMPQAWEEVINILLGIWMVISSWVLGVASRAAETNAVIVGLLVILFAAW
                     AMAMNREWHRNESITPP"
     gene            9927..10715
                     /locus_tag="VFO53_02790"
     CDS             9927..10715
                     /locus_tag="VFO53_02790"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET8875230.1"
                     /translation="MLHNVKELSGLAVGARDGELGKVKDAYFDDERWVIRHLVVDAGG
                     WLNERKVLISPRSVRGVDWNGEVINVELTQQQVKDSPGIDTDRPVSRQHEIDYYHYFG
                     YPNYWEGSNLWGITMLPYPWVGASADPMVAASQPVDSAVARELKGRVDEEIDQADVHL
                     RSCDEVIGYDILATDGSIGEVDDFVFDDHDWALRHLVVDTRKWLPGKHVLLAPQQIDR
                     VSWEDREVYVRLTREEVRNAPELDRSGTSPDAGLERGMGAAPLP"
     gene            10800..12434
                     /locus_tag="VFO53_02795"
     CDS             10800..12434
                     /locus_tag="VFO53_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875231.1"
                     /translation="MADSPAVPRLALRGITKRYPGVLANDRVDLDVLPGEIHAVLGEN
                     GAGKSTLMKIIYGAAEPDAGEIRWEGEAVHVANPARARKLGIGMVFQHFSLFETLTVA
                     DNIRLALEPAQTRQLKQRIRAVSAHYGLPLQPARRVHTMSVGERQRVEIVRCLLQKPR
                     LLIMDEPTSVLTPQATLRLFDTLRTLASEGCSILYISHKLDEIRTLCHRATVLRAGRV
                     TGQCDPRRETTASLARMMIGGELPHTVHREAHVGAAALCVAALDLAADDPFGTRLAGI
                     SFDVCEGEILGIAGVSGNGQKELLAALSGERTLARADAITLGGEPVASLDAFARRRRG
                     LAFIPEDRLGRGAVPEMTLADNALLTVRGNGNVARGWIRTRSVVAFAKQTIAAFGVKA
                     SGPAAVARSLSGGNLQKFIVGREIGQQPKVLIAAQPTWGVDVGASAQIRQALLDLRER
                     GVAVLVVSEELDELIEICDRLAVIAHGHLSPAKPVRETSAEEIGVLMAGAAARDEADA
                     EAIGTIRAEGPPRGANCAPTGGSAAAQPQAWGDHHP"
     gene            12431..13504
                     /locus_tag="VFO53_02800"
     CDS             12431..13504
                     /locus_tag="VFO53_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875232.1"
                     /translation="MKLRLEARPEPSRIALWTAPLLATVATLCVGFIVFTALGRNPLA
                     AFDAFFIAPVATRYGVAELLLKASPLMLIATGVAIGYRANVWNIGAEGQLTLGALAGG
                     GVALAVGDADWPSALLLPAMFVAGALAGMLWAAIPAFLRTRFNANEILTSLMLVYVAT
                     LLLSWLVHGPWRDPEGYNFPQSKIFAANAMLPILLPETRLNAGLVLALAVVALAWLFV
                     RRHIVGFEMRVAGLSPGAANYAGISWRRNIWLGMLAGGACAGLAGVSEAAGPIGQLLP
                     SMSPGYGFAAIIVAFVGRLHPAGIVLASLLMSLLYLGGESAQVNLGLPSAVTGLFEGT
                     LLFFLLAADVLVNYRPRIAATAA"
     gene            13506..14423
                     /locus_tag="VFO53_02805"
     CDS             13506..14423
                     /locus_tag="VFO53_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020396367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875233.1"
                     /translation="MDEIAQIVVLTLAAGTPLVFAALGELVVEKSGVLNLGVEGMMLV
                     GAIASFIVAAKTGAPWLGVGAGALAGALLSLIFGVIALTLRANQVATGLALSLFGIGL
                     SAFIGRPYMSVVIEGIKPLAVPGLTTLPLLGKLVFGHNPLVYVSIALFAAVQIFLRRT
                     RAGLVLRAVGESPQSAHAIGYPVIAIRYGAVLFGGACAGLAGAFLAVAYTPSWVEGMT
                     AGRGWIALALVVFATWKPWRVMIGAYLFGSVTLVQFQAQAAGVAVPAQLLAMLPYVAT
                     VIVLAIISRDATMIRLNAPASLGRPYHPE"
     gene            14504..15598
                     /locus_tag="VFO53_02810"
     CDS             14504..15598
                     /locus_tag="VFO53_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET8875234.1"
                     /translation="MSHITRRTVLATLAAGPIALALPARGQTNAPLKIGFIYVSPIGD
                     AGWTHQHDIARIQMEKNLAPRVTTKYIENVPEGADAERVIRELAQSGHKLIFTTSFGY
                     MNPTVKVAQQFPNTFFEHATGYKTAKNLGTYNARFYEGRYLAGIVAGRMTKSNVAGYV
                     AAFPIPEVVMGINAFTRGMRSVNPKAETKVVWVSTWFDPGRESEAANTLVAQGADVLT
                     HHTDSTAVVQAAEAKKVYAVAYHSDMSKYGPNAQITAVTHVWDRYYTERAQAALDGKW
                     KPDHVWGGIKEGMIAMGPFGKAVPNDVRDTMKKAEAGIATGQLHPFTGPMKDNEGKER
                     LAAEKAITDEALSKMDYYVEGVQGKLPANK"
     gene            15614..16156
                     /locus_tag="VFO53_02815"
     CDS             15614..16156
                     /locus_tag="VFO53_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HET8875235.1"
                     /translation="MATPALRGYGGVAKTLHWLVFALVFAQYVVALAMPDIGRTTVPG
                     TLINLHMSIGATILLVIVVRWLWRIGRPVPLSTVGPRIERDVARVTHALLYVLLAVNP
                     ILGWMNASARDWKITIYGIVTLPHLVAPRARIGRVAGDVHTFLAWTLLVLIGLHVAAA
                     LYHFFLRRDGVLQRMLPGAD"
     gene            complement(16213..>16964)
                     /locus_tag="VFO53_02820"
     CDS             complement(16213..>16964)
                     /locus_tag="VFO53_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8875236.1"
                     /translation="DDDADLALAAPRLALGKLINAGQTCIAPDYALVPKPRLGEFVHA
                     MDAAVRRLYPATVRNADYSSIIDTRHYARLIGLLEDARARGARLIALADAPSGDAELP
                     RRLPPALVLDVSDDMAIMREEILGPLLPVETYSTLDDAIARINARPRPLALYWFGNRA
                     AHRDRVLRETLAGGVTVNDTLWHFAHPGLPFGGVGGSGSGACHGEHGFVRFSHRKPVF
                     VQSRLTATRLLAPPYGRNFERALALLRSPLG"
BASE COUNT         2582 a         5821 c         5818 g         2743 t
ORIGIN      
        1 gcgccgtcag gccgacacgg cccttgtacg ccggattcaa caggtcgtcg taggacttcg
       61 gcggcgtctt gatcttgttc gggttgtagc cgatgccgat cacctgcagc gagatcttcg
      121 ggccccattt atcctggtag accggaaaca gctccttgaa attgggcgac ttcgcggccg
      181 ggtactcgtc gatcaggccc tgcttcgcgg cgtcgaacac ctgcggctcg tcgaggatcg
      241 cgacgtcgaa cggcggccgg ccgcccttcg ccgcctcgag cttcgcgatc tgctcggtgg
      301 cgagctggat gctctgcgtg acgccagcac ccgtgcgctt cctgaaatac ggcgcgagca
      361 ggttgcggtg cgcctcgttc cacgtgccgg ggaacgtggc caccaccagc gacttcgact
      421 gcgcccgcgc gaagggcagc attgcgtaca ggcccgcgcc ggcagcggcg gcgagcaatt
      481 gcctgcggct cataggtgat cccatgacgt tctccctcga gttgaataca cgattgcgac
      541 gtttcgagac ggtcctacgc ggcgcgctcc gattgcgcgg caaacatgtt gcacgcatgc
      601 gctgcgacgg gcgccacatg cacgcattcg cctggcaaca gcagttgcgc gccggcatcg
      661 cgcggcgcgc tgatgcgaaa ggcgacgccg gtgtcggtcc gcgcctcgta catcacgtgc
      721 ggaccgagcg gaagcacgac cctgatcgtt gcagtcactg caccgtcgac gtgcgccgaa
      781 tgaatgcgca gttgctcggg gcgcaccgaa agcaccacac ggtccccctg ccgaagaccg
      841 ggcgcgggtg ctgccgccat caacccgccg ccttcgaggc ggacgacgcc gagcacggca
      901 tcggcgagct cgccgtgcag cagattggtc gtgccgacga actggttcac gaacaccgtc
      961 tgcggccggt cgtacacgtc ggtgggcgat gaaagctgct cgatgcggcc gcggttcatc
     1021 accgcgatgc gatccgccat cgacagcgct tcctcctggt cgtgcgtgac gagaatcgcc
     1081 gtgatgccgt attcgcgctg cagccgcttc acctcgatct gcatgtcgag gcgcaggttc
     1141 ttgtcgagcg cgccgaacgg ctcgtcgagc agcaggattt tcggatcgac ggcgagggcg
     1201 cgcgcgagcg caatgcgctg ctgctggccg ccggagagct gccgcggcag ccgctgcgcg
     1261 tagctctgca tgtgcacgag ccgcagcatg tctgccacgc gcgattcgat ccggtccttc
     1321 gcgaggcgtc tcgcctcgag cccgtaggcg acgttctgcg cgaccgtcat gtgcgggaac
     1381 agcgcgtagt tctggaacac gatgcctgcg ccgcggcgcg tcggcgcgac gtggtcgacc
     1441 gcttcgccat cgaacagcac gcggccttcg ctttgggcga tgaagccggc gatgatgcgc
     1501 agcagcgtcg acttgccgca gccactcggc ccgagcagcg ccacgagctc gccgcccgcg
     1561 atgtcgagct cgatcccctc caccgccgtg acgtcaccga aacgatgcgt cacgcgctcg
     1621 agcgtcagcc ggtggccctt cggcgcgtcg gtcatcgggg aagcacgcgg ccgctcagct
     1681 gccgcggtaa gtcgagtatg cccacggcga gcaaagaagc gggacatggt agtgcgcggc
     1741 gacgtcggcg atggcgaaac ggatcagcac gcggtcgagg aacggcgggt cggcgagggc
     1801 atggccctgg ccgcggaagt agtcggccac gtggaacgtg agctcgtatt cgccgcagcg
     1861 catcgcgtcc gccgggagaa gcggctcggc ggtgcgcccc tcggcattcg tgtacacggt
     1921 gcgcacgccg ggtgccgctt cgcggtcgag cgaagcgaaa tcgacgcgca tgccggccgc
     1981 cggcttgccg gccgatgtgt cgaggacgtg cgtggtgagc tttcccatca gcttgtcgcg
     2041 ccgcctctcg ccgaggcctc tcgacacggt tgctgcggcg aacgcgcgat gattcttgac
     2101 gcccaggatc actgtccaat atattgttcg actcattcaa tacgttttac ctatggatct
     2161 ccgccacctg cgctacttcg ccacactcgc gcaggaattg aatttcacgc gtgccgcgca
     2221 gcgcctcaac gtctcgcagc cgccgctcag ccagcagatc aaggcgctcg aaaccgagct
     2281 cggaacgctg ctgttcgtgc gcaccagccg gcgggtggag ctgaccgccg cgggccatgt
     2341 gttcctcgcc catgccagcg cgattctcga gcgcgtcgag caggcatgcg cccaggcgcg
     2401 cgcggtgggc ggcggccatg tcggccatct cgacatcggc tcgagcagct cgcttttgct
     2461 cggtccgttg ccgcagctca tcgccgggta tcggcgcacc ttccccggcg tgaacatcgc
     2521 gctgcacgag atgacgccgg cggcgcagct cacagcgttg cgcgatcggc gtctcgacat
     2581 cagcttctcg cgcacgcccg tgctcgacca gcacctgtcg gccgagcttg cgtgggagga
     2641 ccccgttgtc gccgcgctgc cgcgcgcgca ccggctcgtt gcgcgacgca agctcgcgct
     2701 gaaggatctc gcgaacgagg agttcgtgct gctgcggctc gattcgtccg cattcgccgt
     2761 gtatctcaac cagtgctgcg tcgatgccgg cttcctgccg cgcgcttccc agtatgtcgt
     2821 cgagtcgcag gcgatgccga gcctcgtggc cgcgggtctc ggtgtggcgc tcgtccccgc
     2881 ctcgcttgcc cgcgtgcacc ggcagggggt cgagtaccgg acgctggccg gtgctgcgcc
     2941 gcgcgccgac gtgtatgcgc tgtcgcgccg cgacgacccg tcggccaccg tgcgcggttt
     3001 tctggaaaag gcccgcgaaa ttctcgccgc gggaaagtag cccgcccgcg cgccaccggc
     3061 gcgacccgct ccggtctgca gccgcagcgc cgtgcacgct gctaccatgc cagggatggc
     3121 gacgccacta ccgcaactcg gcgatctcga cgaaggggcg gctcccttcg acgaattgaa
     3181 tcccgcgccc cggctgctca tgggtccggg gccgatcaat gcgcatccgc gcgtgctgca
     3241 ggcgctcgcc gcgccgatgc tcggccagtt cgacccgcag ttccgcgcgt acatgaaaca
     3301 gacgatgagc ctgtaccggc gcgtgttcga aacgcgcaat cgctggacgc tcctcgtcga
     3361 cggcaccgcg cgggcgggga tcgaatgcgt gctcgcctcg gcggtcgagc ccggcgaacg
     3421 cgtgctcgta ttgtcgttcg gccgcttcgg ccagttgatg atcgaaatcg cccggcgtgc
     3481 gggtgccgac gtgcgcgtcg tcgaaaccgc gtggggcacg gtgttccggc ccgagcaggt
     3541 cgaggcggcg ctcgtgcgcc attcgccgaa gctcgtggcc gtatgccagg gcgatacgtc
     3601 gacgacgatg gcgcagccgc tcgacgcact cggcgcgctg tgccatcggc acggcgcgat
     3661 gcttcaggtc gatgcgaccg cgacgctcgg cggcatgccg ctgccggtcg acgcatggca
     3721 gatcgattgc gccaccagcg gtttgcagaa gtgcctcgcc ggtccgtccg gatcggcacc
     3781 gatcacgatc agcgagcgca tggcgcaaac cgtctaccgg cgccgccaca tcgaggaggg
     3841 cctgcgcccg gcgaactacg tgccgggcga aggcgcgatc gtcgggtcga actatttcga
     3901 tctcgcgatg ctgatcgact actggagcga cctgggcctc aaccatcaca ccgaggccac
     3961 gtcgatgctc tatgcggcgc gcgaatgcgc gcgattgttc ctgcaggagg ggcacgagcg
     4021 cgcatttcgc cgccatgcgc tcgccgggga ggcgctcgcc gccggcgtgg aagcaatggg
     4081 cctgcgcatc ttcggcgacc gcgcgcacag gatgccgaac gtcaccggcg tccacatccc
     4141 cgacggggtc gccggggatc gcgtgcgttc ggcgctcctc gccgatttca acatcgagat
     4201 cgggacgtcg ttcggcccgc tgcagggcaa ggtctggcgc atcgggacga tgggcgtgaa
     4261 cgcgcgcaag gacgcggtgt tgaccacgct cggcgcgctc gaggcggtgc tcgccggcga
     4321 acacgtgaag cttccgcgcg gcgcagcggt cgacgccgcg cgtgccgtct atcgcgaaga
     4381 tgagtgatgc gatccgtttc ctgctgaacg gcgagcgcgt cgatgtcgcc ggcgttgccc
     4441 cccagacgac gctgctcgac tacctgcgcg acgagcgccg cctgaccggc accaaggaag
     4501 gctgtgccga aggcgattgc ggcgcctgca cggtggcact cgccgagcgt gccggcgacg
     4561 cgctcgcgtg gaagcccgtc aacgcctgca tccgactgct gccgtcggtc gatggcaagg
     4621 ccgtcttcac cgtcgagagc ctcgcgtcgg acgacggcgc gccgcatccg gtgcagcagg
     4681 cgctcgtcga atgccacgcg tcgcagtgcg gcttctgcac gcccggattc gcgatgagcc
     4741 tgttcggcct gtacaaaaat gcgtcacgac cgtcgcgcaa cgccgtcgac gatgcgctgt
     4801 ccggcaatct gtgccgctgc accggctacc ggccgatcgt cgatgcggca atgcgcatgt
     4861 acgcgctccc acccgctgcc ggctggcgtg gccgcggcgt cgccgacgac ggcacgcgca
     4921 tcgtcgcgcc ggacgaagcg cgtctggcgg ccgatcttgc actgctcgcg cgcgacgaca
     4981 cgttcgacta cgcgcatgcg ggccggcgct ggtcggcgcc gcgcacgctt gcagccctcg
     5041 ccgatgcctg cacggctcgt ccgcaggcgc acgtcgttgc cggcatgacc gacgttgcgt
     5101 tgtgggtcac gaagcagcat cgggatctcg acgacgtggt ctatctcggg gatgtcgacg
     5161 atctgcacgc gatacgcgag accggcgccg gcgtcgagat cggtgcggcg gtcacgctca
     5221 ccgacgcgtt gccgacgctc gacgcgctgt tccccgaatt gcacgaagcg tggcagcgct
     5281 tcgcatcggt gccgatccgc aacagcgcga cgctcgcagg caacgtcgcg aacggctcgc
     5341 cgatcggcga ttcgatgccc gcgctcctgg cactcgacgc gcgcgtgctg ctgcgaagcg
     5401 cggccggcga acgcgaagtg gcgctcgacg ccttctaccc gggctaccgg cagacggcgc
     5461 gggtgccggg cgaattcatc gccgccgtgc gcatcccgcg acgcgtcgaa ggactgctgc
     5521 tcggcgctta caagatcagc aaacgctacg accaggacat ttccgccgtc ttcgcatgct
     5581 tcgcactcgt gctcgacgca ggacgcattc gcggtgcgcg catcggctgc ggcggggtcg
     5641 cggcaacgcc ggtacgcgcg caccgcgcgg aagccacgct cgtcgacgcg atgtgggatc
     5701 gtgcaacggc cgagcatgcc gcgcagacgc tcaccgggga gttcgcgccg atcgacgaca
     5761 tgcgcgcgtc ggccgcctat cgccgcacgg tcctcggcaa cctgctgcgc cgcttctggc
     5821 tcgaatccgc ggcgaggccc gatgcgccga cgcgcgtcga gatgctcctg ccgcagacgc
     5881 gatgaacgca ccccgtccgc catgggattc ggcgaccggc gtcgtcggcg tgcccgcggc
     5941 gcacgattcc gcggcgctgc acgtcgcggg cgaagcgcac tacaccgacg acctgcccga
     6001 gccgcgcggc accctctacg cggcactcgg cctcagcccg cacgcgcatg gaaggatcga
     6061 gcgcctcgac ctcgacgccg tgcaaggggc gccgggcgtc gtggcggtgg tgaccgcggc
     6121 ggatttcatc gtcaacgacg tggggccgat cgaggccgac gatccgatcc tcgccgatcg
     6181 catcgtgcaa ttcgcggggc agccggtgtt cggcgtcgcc gcgacgagcg tgggtgccgc
     6241 gcgccgtgca gcgacgctcg cacgcctcga cgtcgcaccg ctgcccgcga tcctgacgat
     6301 cgacgatgcg ctggccgcgc aatcgtttgt cctgccgccg gtgcatgtca cgcgcggcga
     6361 tccgcaggaa gcgctcgcgc gcgcgccgca tcggctcgcc ggccgcgccg caagcggcgg
     6421 acaggatcat ttctatctcg aaggccaggt cgcgctcgcc gtgccgcgcg agcatgacgg
     6481 catgcagctc ttcgtctcga cgcagcatcc gggcgaggtg cagatgctgg tcgcccgcgc
     6541 gctcgcgctg cgcgtcaacg atgtcgtcgt cgagtgccgg cgcatgggtg gcgcgttcgg
     6601 cggcaaggaa acgcagatgt cgctgttcgc ctgcgttgcc gcgttgctcg cccgcaggac
     6661 cgggcgcgca gtcaagcttc gcctcgaccg cgacgacgac atgcgcgcca cgggcaagcg
     6721 gcatgcattc gaatatcgct acgacgcggg cttcgacgac gcgggacgca tcctggcact
     6781 cgacgtcacg ctggcgtcgc gctgcggctt ctcggccgac ctgtccggtc ccgtcaacga
     6841 ccgcgcggcg tttcacgccg acaatgcgta ctggctgccg aacgtcgcca tccattcgta
     6901 tcgctgccgc acgaacacgg tgtcggatac cgcgtttcgc ggcttcggcg gtccgcaggg
     6961 catgttcgcg atcgaaaccg tgatcgacga catcgcgcgc acgctggcgc tcgatccgct
     7021 cgacgtgcgc aaggccaacc tctacggcac caccgatcgc aacgtgacgc cgtacggaat
     7081 gagcgtcgag gacaacatcg cgcccgtgct gatcgacgcg ctggagacac gtgccgacta
     7141 ccgggcgcgc aggcgcgcca tcgcgcaatg gaatcgcgaa agtccggtga tcaagcgcgg
     7201 catggcgctt acgcccgtca agttcggcat ctcgttcacc gcgacgcact acaaccaggc
     7261 gggcgcgctg atccacgtgt accgcgacgg cagcgtgctg ctcaaccatg gcggcaccga
     7321 gatgggccag ggcctgttcg tcaaggtgca gcaggtcgtc gcacacgagc tcggcgtcgc
     7381 gctggattcg gtgcgcgtgt cggcgtcgga cacgagcaag gtgccgaatg cgtcgccgac
     7441 ggccgcctcg tcggggagcg acatcaacgg catggcggcg cgcgctgccg cgcgcaagct
     7501 gcgcgagcgg ctcgccgcct tcgccgcagg cgaaagcggt gtcgcggtcg agcacgtgca
     7561 tttcgccggc gggatggtgg tcgcgggcag cgagcgaacg acgtttgcgg cgctcgtcga
     7621 cagggcgtac cacgcgcgca ttccgttgtc tgccaccggc ttctacgcca caccgaagat
     7681 tcactacgac cggcgcacgc tgtccggccg tccgttcttc tatttcgcgt acggcgcggc
     7741 cgtcaccgag gtcgcgatcg acacgctgac gggcgagcat cgcctttgcg cggtcgacat
     7801 cctgcacgac gtcggcgcgt cgctcaaccc ggccatcgac cgcggacaga tcgaaggcgg
     7861 gttcatccag ggatacggct ggctgaccat ggaagagctt tgctggaacg cgcgcggcga
     7921 gctcaccacg cacgcgcctt cgacgtacaa gatcccgacg tcccgcgacg ttcccccgca
     7981 cttcgacgtc gtttttttcg gcgaaccgaa ccgcgaggaa acgatccatc gctcgaaggc
     8041 ggtcggcgag ccgccgttga tgctcgcgct ctcggcattc cacgcgctgc gcgacgcgat
     8101 cgcgagcgtt gccgaccagc ggctcgtgcc gcgattggac gcacccgcga cgccgcaacg
     8161 cgtgctcgcc gcgatcgacg acgtgcgagc gcgggatcgc gcgacgccga gggcgtgatg
     8221 gacgacgcca acacgcttcc ctggctcgac gcgctcgccg cgacgctcgc gcgcgaccgg
     8281 gaggcggtgc tgatcgtcgt cgccacgacc agaggctcga cgccgcggga aagcggtgcg
     8341 gcgatggtcg tctcgcgcga cgcatgcgcc ggatcgatcg gcggcggaca cctcgaattc
     8401 gaagcaacgc gcattgcgcg cgacgcgctg tgcgatccgg cggcggcggg cggcacgtgg
     8461 atcgtgcgct tcccgcttgc ggcgcgcctc ggccaatgct gcggcggcgt cgcatcgctc
     8521 gcctttgcgc ggctcggcgc caacgccaat gcgtggctcg agcccgccat cgcgtgcgca
     8581 cgtgcttcca cgccttttgc gatcgtgacg cgcatcggcg gcgccccggc gtccgagggg
     8641 cggctcgtgg tgaccagcga cgacgtgcgt ggcagcctcg ggaacgtggc gctcgattcg
     8701 ggcgcaatcg ggcttgcgcg tgcgcgtctt gccgcggcga gcaacggcgc cgcgatcgtt
     8761 cgcttcgccc gcgatgacga cgcgccgctg ctgatccagg tcgagcggtg cgacgagttc
     8821 ccggtcctgg tgttcgggaa cggccacgtc ggccgtgcgc tggtgagcgt gctcggcgtc
     8881 gtgcccgcgc aggtacgctg gatcgacgcg cgcgccgacg actttgcgcg gcgcattccc
     8941 ggcaacgtcg aggtcgtcgt gaccgatgcg cccgaagacg agcttgccga tgcgccgcac
     9001 ggtgcgtacg tcgtcgtgac cacgcacagt cacgcgctcg attttgccct gatcgaggca
     9061 gcactcgcgc gcgacgactg gcgctacgtc ggactgatcg gctcgaagtc gaagcgtgcg
     9121 cagttcgagc ggcggcttgc cgcgcgcggc ctcggcgacg aagctttcgc gcgcattcgt
     9181 tgcccgatcg gcagcgaggt cgctattcgc gccaagcatc ccggcgcgat cgccatcgcg
     9241 atcgccgccg aactgctcgt cgttcgcgag agcggcgcac gccgcgccgg cgtcccgcgc
     9301 gaattcgcgc ttgtccgcaa gggttgagcg cccgctgtcc tgaaatgcgc gttgtaattc
     9361 gtgcaatatt tttgtcgccg ctcgccgacg gcgggggaat ccttcgcgca ccggtacatc
     9421 aaacaatcat caggtaaccg gaggtgacgt catgaaggaa aaacgctggc aggactggct
     9481 caatctgctg atcggcatct ggctgttcat ttcgccctgg gtgatcgggt tcgtcggcac
     9541 cgacgcgtct gcgtcatgga acgcctggat tctcggcgtt gcgatcgtcg tgttctcggc
     9601 cattgccgtt tcgatgccgc aggcgtggga agaggtgatc aacatcctcc tcggcatctg
     9661 gatggtgatc tcgtcgtggg tgctcggggt cgcgagccgt gcggccgaaa ctaacgccgt
     9721 gatcgtgggt ctgctcgtca tcctgttcgc cgcgtgggcg atggcgatga accgcgagtg
     9781 gcaccgcaac gagtcgatca ccccgccgta gcggcggcgc ggagccacct tgctcgctcg
     9841 cgacgcgagc aggcgaaaca gatgcagcgg gcgaatggcg catctcgcgc cattcgcttc
     9901 gccgtgtctt ctctcggagc gatgacatgc tgcacaacgt gaaggaactc tcgggcctcg
     9961 ccgtcggcgc gcgcgacggc gagctcggca aggtgaagga cgcgtacttc gacgacgagc
    10021 gctgggtcat acgccatctc gtcgtcgatg ccggcggctg gctgaacgaa cgcaaggtcc
    10081 tgatctcgcc gcggtcggtg cgcggcgtcg actggaacgg cgaggtcatc aacgtggagc
    10141 tcacgcaaca acaggtgaag gacagtccgg gcatcgacac cgaccggccc gtgtcacgcc
    10201 agcacgagat cgattactac cactacttcg gctaccccaa ttactgggaa ggctcgaacc
    10261 tgtggggcat cacgatgctg ccgtatccgt gggtcggcgc atcggccgat ccgatggttg
    10321 ccgcgtcgca accggtcgac tcggccgtgg cgcgcgaact aaagggacgc gtcgacgagg
    10381 aaatcgacca ggccgacgtg catcttcgca gttgcgacga ggtgatcggc tacgacatcc
    10441 tcgccaccga tgggtccatc ggcgaggtcg acgacttcgt gttcgacgat cacgactggg
    10501 ccctgcgcca tctcgtcgtc gacacgcgca aatggctgcc cgggaagcac gtgctccttg
    10561 cgccgcaaca gatcgaccgg gtgagctggg aggatcgcga ggtgtacgtg cggctgaccc
    10621 gcgaggaggt ccgcaatgcg cctgagctcg atcgctccgg cacgtccccc gacgccggcc
    10681 tcgagcgggg catgggcgcc gccccgctgc catgacgtga acggggtggc cgccttgcgc
    10741 gagccacccc tgccgccgac gatttcgcaa aggaggcgcc gtcccggata atccgcggca
    10801 tggcggactc cccggctgtc ccgcgtctcg cgctgcgcgg catcaccaag cgctatcccg
    10861 gcgtgctcgc caacgatcgc gtcgacctcg acgtgctgcc gggcgagatc cacgccgttc
    10921 tcggagagaa cggcgccggc aagtcgacgc tgatgaagat catctacggc gccgcggagc
    10981 ccgatgccgg cgagatccgc tgggaaggcg aagcggtgca cgtcgcgaac ccggcacgcg
    11041 cgcgaaagct gggcatcggc atggtgttcc agcatttctc gctgttcgag acgctgaccg
    11101 tcgccgacaa catccggctc gccctcgagc ctgcgcagac gcgccagctc aagcagcgca
    11161 tccgcgccgt atcggcgcac tatggccttc cgctgcagcc ggcgcgtcgc gtccacacga
    11221 tgtcggtagg cgagcggcag cgcgtggaga tcgtgcgctg cctgctgcag aagccgcggc
    11281 tgctgatcat ggacgagccg acgtcggtgt tgacgccgca ggcgacgctg cgcctgttcg
    11341 acacgctgcg caccctggcg agcgaaggct gcagcatcct ctatatcagc cacaagctcg
    11401 acgagatccg cacgctttgc caccgcgcaa cggtccttcg cgcgggaagg gtcaccgggc
    11461 aatgcgatcc gcgccgcgaa accacggcgt cgctggcgcg aatgatgatc ggcggcgagc
    11521 tgccgcacac ggtgcaccgc gaagcgcacg tcggcgccgc ggcgctttgc gttgctgcgc
    11581 tcgacctggc ggccgacgac ccgttcggca cgcggctcgc cgggatttcg ttcgacgtct
    11641 gcgaaggcga gatcctcggc atcgccggcg tctccggcaa cggccagaag gagttgcttg
    11701 cggcgctgtc cggcgagcgc acgctcgctc gcgccgatgc gatcacgcta ggcggcgagc
    11761 cggtcgcgtc gctcgacgca ttcgcccgtc gccggcgcgg gcttgcgttc atccccgagg
    11821 atcggctggg ccgcggcgcc gtgcccgaaa tgacgctcgc cgacaatgcg cttctcaccg
    11881 ttcgcgggaa cggcaacgtc gcgcgcggct ggatccgcac gcgcagcgtc gtcgcgttcg
    11941 cgaagcaaac gatcgccgcg ttcggcgtca aggcgagcgg ccccgcggcg gtcgcgcgca
    12001 gtctctcggg aggcaacctg cagaagttca tcgtcggccg cgagatcggg cagcagccga
    12061 aggtgctcat cgcggcgcaa ccgacgtggg gtgtcgacgt cggcgcgtcc gcgcagatac
    12121 gccaggcgct gctcgacctg cgcgagcgcg gcgtggccgt gctcgtcgtc tcggaagagc
    12181 tcgacgagct gatcgagatc tgcgaccggc ttgcggtgat cgcgcacggc cacctgtcgc
    12241 cggcgaaacc ggttcgcgag acgagcgccg aggagatcgg cgtgctgatg gcgggcgcgg
    12301 cggcgcgtga tgaggctgac gctgaggcca tcgggaccat tcgcgccgaa gggccgcccc
    12361 gaggcgcgaa ttgcgcaccc accgggggca gcgcagcggc gcagccgcaa gcgtgggggg
    12421 accaccaccc atgaaattgc gactcgaagc acggcccgag ccgtcgcgca tcgcgctgtg
    12481 gacggccccg ctgctcgcca cggtggcgac gctttgcgtc ggcttcatcg tgttcaccgc
    12541 gctcggcagg aatccgctcg ccgccttcga cgcgttcttc atcgcgcccg ttgccacgcg
    12601 ctacggcgtc gcggagctgc tgctcaaggc gagcccgctg atgctgatcg ccaccggcgt
    12661 tgcgatcggc tatcgcgcga acgtctggaa catcggcgcc gagggccagt tgacgctcgg
    12721 cgcgctcgcc ggcggcggcg tcgcgctcgc ggtgggcgac gccgactggc cgtcagcact
    12781 cctgctgccc gcgatgttcg tggcaggcgc gctcgccggc atgctgtggg ccgcgattcc
    12841 cgcattcctg cgcacccgct tcaacgcgaa cgagatcctg acgtcgctga tgctggtcta
    12901 cgtcgcgacg ctgctcctgt cgtggctcgt gcacgggccg tggcgcgatc ccgagggata
    12961 caacttcccg caatcgaaga tcttcgcggc gaacgcgatg ctgccgatcc tgctccccga
    13021 gacacggctc aacgcgggac tcgtgctcgc gctcgccgtc gtcgctctcg cctggctgtt
    13081 cgtgcgccgg catatcgtgg gcttcgagat gcgggtcgcc ggcctgtcgc ccggtgccgc
    13141 gaactacgcg ggtatctcgt ggcggcgcaa catctggctc ggcatgctcg caggcggtgc
    13201 gtgcgcggga ctcgcgggcg tcagcgaagc cgcgggaccg atcggtcaat tgctgccgtc
    13261 gatgtcgccg ggctacggct ttgccgcgat catcgtcgcc ttcgtcggcc gcctgcatcc
    13321 ggccggcatc gtgctcgcga gcctgctgat gtcgctgctg tacctcggcg gcgaatcggc
    13381 gcaggtgaac ctcgggctgc cttccgccgt gaccggactg ttcgagggga cgctgctgtt
    13441 cttcctgctc gcggccgacg tgctcgtcaa ctaccggccg cgcattgcgg cgaccgcggc
    13501 ctgaagtgga cgagatcgcg cagatcgtcg tgctgacgct cgccgccggg acgccgctcg
    13561 tgttcgcagc gctcggcgag ctcgtcgtcg agaaatccgg cgtgctcaac ctcggcgtcg
    13621 agggcatgat gctcgtgggc gccatcgcat cgttcatcgt cgcggcgaag acgggcgcgc
    13681 cgtggctcgg tgtcggtgcc ggtgcgctgg ccggcgcgtt gctgtcgctg atcttcggcg
    13741 tcatcgcatt gacgctgcgt gcgaaccagg tggcgacggg gctcgcgctg tcgctgttcg
    13801 gcatcggcct gtccgcgttc atcgggcgcc cgtacatgag cgtcgtcatc gagggcatca
    13861 agccgctggc ggtgccgggc ctcacgacac tgccgcttct cggcaagctc gtcttcggcc
    13921 acaacccgct cgtctacgtg tcgatcgcgc tgttcgccgc cgtccagatc ttcctgcgcc
    13981 gcacgcgtgc gggcctcgtg ctgcgcgcgg tcggggaatc gccgcagtcg gcgcacgcga
    14041 tcggctatcc ggtgatcgcg atccgctacg gggcggtgct gttcggcgga gcctgcgccg
    14101 gcctcgccgg cgcgtttctc gccgtcgcct acacgccgtc gtgggtcgag ggcatgacgg
    14161 cgggccgcgg ctggattgcg ctcgcactgg tcgtgttcgc gacgtggaag ccttggcgcg
    14221 tcatgatcgg cgcctacctt ttcggaagcg tcacgctcgt gcaattccag gcgcaggccg
    14281 ccggcgtcgc ggtgcccgcg cagctgctcg cgatgctgcc gtacgtcgca acggtgatcg
    14341 tgctcgcgat catctcccgc gacgcgacga tgattcggct gaatgcgccg gcatcgctcg
    14401 gcaggccgta ccatccggag tgatcgcggc ggccgattcg agggcccggt gcccacggcg
    14461 tacaatcccg gcccattccg cgccctcgcc caaggagtcc tcgatgtctc acattacccg
    14521 cagaacggtg ctcgcaacac ttgccgccgg tccgatcgcg cttgccctgc ccgctcgcgg
    14581 ccagacgaat gcgccgctca agatcggctt catctacgtg agcccgattg gcgatgccgg
    14641 ctggacgcac cagcacgaca tcgcgcgcat ccagatggaa aagaacctgg cgccgcgcgt
    14701 gacgacgaag tacatcgaga acgtgcccga aggcgccgac gccgagcgcg tgatccgcga
    14761 gctcgcgcaa tcggggcaca agctgatctt cacgacgtcg ttcggctaca tgaacccgac
    14821 ggtcaaggtc gcgcagcagt tcccgaacac gttcttcgag catgcaaccg gctacaagac
    14881 cgcgaagaac ctgggcacgt acaacgcacg cttctacgag ggccgctacc tggccgggat
    14941 cgtcgcgggc cggatgacga agtcgaacgt tgccggctac gtcgccgcgt ttccgattcc
    15001 cgaagtcgtg atgggcatca acgcattcac ccgcggcatg cgcagcgtca atccgaaggc
    15061 ggagaccaag gtcgtttggg tgagcacctg gttcgatccg ggccgcgaga gcgaggcggc
    15121 gaatacgctc gtcgcacagg gcgccgacgt gctgacgcat cacaccgatt cgacggcggt
    15181 cgtgcaggcc gccgaagcga agaaggtcta cgcggtggcc tatcactcgg acatgtcgaa
    15241 gtacgggccg aacgcgcaga tcaccgcggt aacgcacgtc tgggacaggt actacaccga
    15301 gcgcgcgcaa gcggcgctcg acgggaagtg gaagcccgat cacgtgtggg gcggcatcaa
    15361 ggagggcatg atcgcgatgg gtccgttcgg taaggcggtg ccgaacgacg tgcgcgacac
    15421 gatgaagaag gcggaagcgg gcatcgccac gggacagctt catccgttca ccgggccgat
    15481 gaaggacaac gagggcaagg agcgtctcgc cgccgaaaag gcgatcaccg acgaagcgct
    15541 atcgaagatg gactactacg tcgaaggcgt gcaggggaag ctgccggcga acaagtagca
    15601 cgggcagccg gacatggcca cgcccgcgct gcgcggctac ggcggcgttg cgaagacgct
    15661 ccactggctc gtcttcgcgc tcgtcttcgc gcagtatgtc gtcgcgctcg cgatgccgga
    15721 catcggacgc acaacggtgc ccggcacgct gatcaacctg catatgtcga tcggcgcgac
    15781 gatccttctc gtcatcgtcg tgcgctggct ctggcgcatc ggccgcccgg tgccgctttc
    15841 gaccgtcggg ccccgcatcg aacgcgatgt cgcgcgcgtc acgcacgcgc tgctgtacgt
    15901 gcttctcgcc gtcaacccga tcctcgggtg gatgaatgcg tcggcgcggg actggaagat
    15961 cacgatctac ggcatcgtca cgctgccgca tctggtggcg ccgcgcgccc gaatcggcag
    16021 ggtggccggc gacgtgcaca cgttcctcgc ctggacgctg ctcgtgctca tcggcctgca
    16081 tgtcgccgcg gcgctttatc acttcttcct gcgccgcgac ggcgtgctgc aaaggatgct
    16141 gcctggcgcg gattgactga cggaacaatc gttcgcgggc acggcaggcg cgttctgcga
    16201 tgacgggctt cgtcagccga gaggcgaacg cagcagcgca agcgcgcgct cgaagttgcg
    16261 cccgtacggc ggcgcgagca ggcgcgtcgc ggtaagccgc gactggacga acacgggctt
    16321 gcgatgcgag aagcggacga acccatgctc gccatggcaa gcaccgctcc ccgagccgcc
    16381 gacaccgccg aacggcaggc ccggatgcgc gaagtgccaa agcgtgtcgt tgaccgtcac
    16441 gccacccgcg agcgtttcgc gcagcacgcg gtcgcggtgt gcggcacggt tgccgaacca
    16501 gtacagcgca agcggacgcg gccgcgcgtt gatcctggcg attgcgtcgt cgagcgtcga
    16561 gtaggtctcg accggaagga gcggcccgag gatctcctcg cgcatgatcg ccatgtcgtc
    16621 gctgacgtcg agcacgagcg ccggcggcag acggcgcggc agctccgcgt caccggacgg
    16681 cgcatcggca agcgcaatca gccgtgcgcc ccgcgcgcgg gcgtcttcga gaagcccgat
    16741 caggcgcgcg taatggcgcg tgtcgatgat cgacgagtaa tcggcgttgc gcaccgttgc
    16801 gggatagagc cgccgcaccg cagcgtccat cgcatggacg aattcgccga ggcgcggctt
    16861 cggcaccagc gcgtaatcgg gcgcaatgca tgtctggccc gcattgatga gcttcccgag
    16921 cgcgagccgc ggcgcggcca acgcaagatc ggcgtcatcg tcga
//
LOCUS       DASSVX010000061        17217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1202103, whole genome shotgun sequence.
ACCESSION   DASSVX010000061 DASSVX010000000
VERSION     DASSVX010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 17217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17217
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202103"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /gene="dprA"
                     /locus_tag="VFO53_02825"
     CDS             complement(<1..773)
                     /gene="dprA"
                     /locus_tag="VFO53_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET8875237.1"
                     /translation="MALDSRTLAWATLAHKTLPQRAVAALLRQFGSPEAALAASHRQL
                     ASVVPPAVAERTLASVERELLEHTQAWLDHPEHHLIAWDDGDYPPALLELGHAPPVLF
                     HMGRRELLAHPAFAIVGSRHATPQGRETAREFAHALGSAALTIVSGLAVGIDAAAHEG
                     ALETTGSTLAVVGTGLDRVYPARNRDLAARIARNGALVSEYVPGTPARKENFPQRNRL
                     ISGLARGVLVVEATLSSGSLITARLAGEQGRDVFAIPGS"
     gene            complement(849..1931)
                     /locus_tag="VFO53_02830"
     CDS             complement(849..1931)
                     /locus_tag="VFO53_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET8875238.1"
                     /translation="MATRIAALCGVLSILALAAPVSAQTVGIADNAPSTYTVQKGDTL
                     WGIAGRFLKEPWRWPEVWRMNREEIRNPHRIYPGDVIRLTVVDGSPRLDLARNTVRLS
                     PEIRVSPLAPEAIPAIPPGDIEPFLSRPLVTGPEGLGDAAEIVAGRDERVIRGQQDIV
                     YAVGLDPKGGDLWFVYRPGERLVNSAGETLGFENRFLGTVRVERFADVSTARIESATE
                     EIVIGDRLLPATREIVQNYIPHAPDHVIDGRILKLARDVQETGRGYVVTLDKGARDGL
                     EVGNVLAIYRVVAPIRDPRRNKTEPTVQFPNLDQTTFYTPPAYLHVPDERTGILMVFR
                     VFDRVSYALVLNSSGAVRTGDFVRTP"
     gene            2059..2562
                     /gene="def"
                     /locus_tag="VFO53_02835"
     CDS             2059..2562
                     /gene="def"
                     /locus_tag="VFO53_02835"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET8875239.1"
                     /translation="MALLPILEYPDPRLKKVATPVAAVTADVRRLVRDMADTMYAAPG
                     VGLAATQVDVHKRVIVIDISEHKDELRVFINPELLSADGEAECEEGCLSVPGYYDKVT
                     RAARIRVRAQDEHGETFELDAEGLLAVCIQHEMDHLQGKVFVEYLSPLKRARLAAKLR
                     KKQRLAG"
     gene            2577..3518
                     /gene="fmt"
                     /locus_tag="VFO53_02840"
     CDS             2577..3518
                     /gene="fmt"
                     /locus_tag="VFO53_02840"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET8875240.1"
                     /translation="MRVGFAGTPAFAARALAAVHAKGFTIPVVLTQPDRPFGRGLAIA
                     ASPVKRYAADHALPLRQPPTLKLDTVQAALRDIPIDVLVVAAYGLILPQPVLDWPRHG
                     CLNVHASLLPRWRGAAPIARAVEAGDAMSGITIMQMNAGLDTGAVVTERALALGARET
                     TGTLTGRLAILGASMIVDALCTLERDGRLQATPQPSDGATYAAKIGRADTLTDWTQDA
                     EALDRRIRALAPSPGALAGWRGKPVKIHAAVPVEAKVAADAGTVVALGADGIDVACGS
                     GVLRLTQLQPAGGRSMSAQAFAAGHRVAAGERFEPGR"
     gene            3525..4820
                     /gene="rsmB"
                     /locus_tag="VFO53_02845"
     CDS             3525..4820
                     /gene="rsmB"
                     /locus_tag="VFO53_02845"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET8875241.1"
                     /translation="MKQEQTQAALAVMRVFDGAQLRDALALVDDGSALRGRTLVQEIA
                     YGTLRHWGALDAIVGRLARKPIADPLLRTLVAVALYQLEHTRAPPFAVVDRAVAAAGD
                     AVRPAAKALVNALLRRYLRERDEVQCAIAQSDVARWSYPQWWIERVRRDYPAQWQAIL
                     EAGNARAPLTLRVNRRVTSREALAERFADAGMAVEPVAECALIVGVPRPVRELPGFDD
                     GAFAVQDLAAQLAAPLLDVHDGMRVLDACAAPGGKTTHLLESADADVLALDIDTRRLA
                     LIRENVERLRLGAHRLRLVEGDAGNPAAWWDSRPFDRILLDVPCTASGVVRRHPDGKW
                     RHRAGDVDRFAREQARLLAAAWPLLAPGGKLLYATCSVFVAENGAQVAKFVAQHSDAL
                     RETLNFPSATGARDGQLLPSPEAASHNQDGFFYALLRKR"
     gene            4895..5491
                     /locus_tag="VFO53_02850"
     CDS             4895..5491
                     /locus_tag="VFO53_02850"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HET8875242.1"
                     /translation="MPSSLRTDRAPATPRRRFLIALIALIGSAWLAQPVAADEIPVKS
                     ASLRVDEADVLLSAEFDLSLTPALEQALDKGVALYFTIEFELLRTRAFWFPEKLADWS
                     ITYRVSYSPLTRQYRVASGPLGQAFESLDDVERFIGHVASRPVAQASDLVKGASYEAA
                     VRMKLDVNQLPKPFQLNALASREWQLASDWYRFGFTPS"
     gene            5505..7658
                     /locus_tag="VFO53_02855"
     CDS             5505..7658
                     /locus_tag="VFO53_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8875243.1"
                     /translation="MNILASPTLRWVTLGFACLSAIALFLLASATANTDFFAGHYDAL
                     VVINGALVALLMLVVGGQLLQLWRSLRAGVFGSRLALRLVLLFSLVAVLPGALVYAVS
                     VQFLGRSIESWFDVRVDRALDGGINLGRSALDYLLKETTNRATLISGAIADAQGAPGA
                     ALSRAAEQVGVYEAALFAPSGSVVAVAGLGGGMTPEALPGDALRRARLQQTYAKVEQQ
                     QDGGLMLRVVVPVNSGDRIEPLRVLQVIERVPRTLAQDLEKVQAGARDYQEISFSRGA
                     LKRLYQLTLTLTLLLALTSALGLALVLSERFASPLGLLAQGTRAVAEGDFTRRHPVTS
                     RDELGVLTESFNTMTAQLADAKAKTEESQRATETTRAYLESVLSNLSSGVLAFDDRYR
                     LRTANPSAAVILQQPFAELAGVPLAEWGGRLPALAPFGELVAEAFRSAREGHWQREAS
                     LGVAAQTRTLLLRGTRLPGAPVPGYVVVFDDVSELLQAQRDAAWSEVARRLAHEIKNP
                     LTPIQLSAERLTLKLGPHLDEADRETLRRGTQTIVSQVAAMKHMVDDFAIYARKARPG
                     QMQAVDLASLLLDVLALYDNLRPHVALALPDAPVMIQGEPTRLRQVIHNLLQNAVDAQ
                     SGQDESQIDIALSVGDGEAILAFSDRGPGFSENVLAHPFEPYVTTKAKGTGLGLAIVK
                     KIVDEHAGRVTLENVAPRGARVTLHLRALKSVAAA"
     gene            7655..8962
                     /locus_tag="VFO53_02860"
     CDS             7655..8962
                     /locus_tag="VFO53_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8875244.1"
                     /translation="MNPARAAQGREILVVDDEVGIRELLSEILQDEGYRVAVAENAGE
                     ARAYRQRQQPALVLLDIWMPDTDGVTLLREWGSSSLLTMPVIMMSGHGTIETAVEATR
                     IGAFDFLEKPIGLSKLLSTIARAFKSAAAKEPRRISLAALGSGPAIHDAERALEALLA
                     ARRAVLIAGEPGTGHDVAARSLQVPGAPFVALANGARLANNPLALIEEAREGVLYCPE
                     IGQYSRAEQKGLAFLLPKLDKHNIVLVATSAEPLANLATEGKFDAGLLSQLSAGAIVL
                     PPLRSRRDDIPALIERFWRSGGAEERATPLLATLSPAALTILSSAYWPGNLDHLQSVV
                     ANMQIVRGEISADHVKRLLGEAASPNQVVAPEITTRFFELPLREAREAFERIYFEQLL
                     GREQSNMSKVAERAGLERTHLYRKLKQLNIRFSRRTDDSSAVS"
     gene            8986..10875
                     /locus_tag="VFO53_02865"
     CDS             8986..10875
                     /locus_tag="VFO53_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HET8875245.1"
                     /translation="MSSAVASARPPARMLPLALGAVGVVYGDIGTSPLYTLRTAFTGS
                     YGLSLTPDNVLGVVSVIFWALLIVVTLKYITLIMRADNRGEGGILALTALVSRGIESR
                     DRLRWWLVGLGIFGAAMFYGDGMITPAISVLGAVEGLRVIAPAMHPFIVPATIVILVV
                     LFAAQRKGTAGLGAVFGPVMVIWFIALALLGALSIGRNLAVLEALEPVYAFRFIAGNP
                     GTAFVAFGAVVLAVTGTEALYADMGHFGRSPIRRAWLFFVFPALLLNYFGQAALVLDN
                     PAAIENPFYLLAPEWGRVPLLLLATAAAIIASQAVISGAFSLTRAAIQMGYCPRLTIM
                     HTSERAIGQIYIPFINWMLLAAIIGLVVGFGNSDNLAGAYGIAVTMAMLIDSILIFVV
                     MRRLWNWPVPIAILIATPLLLIDLTFLASNALKIPEGGWFPLLIGAVVFTLLTTWKRG
                     RSLLMRRLADDAMPLELFIQSIEASPPTRVPGTAVFLTSTQNRVPHALLHNLKHNKVL
                     HERVVFLTIVTEDIPYVPDEERYEIKPLGCDFFRMVANYGFKDDPDVPELLEDCGRRG
                     FVFDMMETSFFVSRETLIATVTPGMALWREKLFVSMSKNATKASEFFQVPTNRVVELG
                     TQVEL"
     gene            complement(10945..11799)
                     /gene="rpoH"
                     /locus_tag="VFO53_02870"
     CDS             complement(10945..11799)
                     /gene="rpoH"
                     /locus_tag="VFO53_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HET8875246.1"
                     /translation="MSGSALAFPTPASLGSLDHYIQAVNRFPLLSPEQETELGRRWRE
                     HEDVDAARQLVLSHLRLVVAVSRNYLGYGLPQADLIQEGNIGLMKAVRRFDPERGVRL
                     VSFALHWIRAEIHEHVLRNWRLVKIATTKAQRKLFFNLRSMKTSSAALTPDEANKIAA
                     DLGVKPEEVFEMEKRISGGDVSLDPTPGDEDSVSPIAYLSDAEDEPSQILERAQMAAN
                     RTAGLRSAMSKLDARSRRIIEARWLREEDSATLQELANEYGVSAERIRQIESKALKTM
                     RSEMVTLQ"
     gene            complement(12049..13107)
                     /gene="ftsY"
                     /locus_tag="VFO53_02875"
     CDS             complement(12049..13107)
                     /gene="ftsY"
                     /locus_tag="VFO53_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET8875247.1"
                     /translation="MFDLFKRKPAPQPAAPAESAPPEPVAGVPGVPSAPPSEAAAGVS
                     AAKRSWRERLKSGLGLSREKLSGALSGALRRRVLDDEALEALESALLMADVGIAATQH
                     LLDELRLRYRKAGADADPRALLRGAFADLVRTLEAPVTVGAARPFVIMLAGVNGAGKT
                     TSIGKIAKWLQQRHLSVLLAAGDTFRAAAREQLVVWGERNNIAVIQQAGGDPGAVMFD
                     AVGAASSRGLDVVLADTAGRLPTQAHLMDELRKIKRVLGKAREGAPDEVWLVLDANTG
                     QNALAQVKAFDAAVGLTGLILTKLDGSAKGGIVAAIARQHPIPLRFIGVGEGVDDLRP
                     FDADEFVDALLPAEEALS"
     gene            13219..13770
                     /gene="rsmD"
                     /locus_tag="VFO53_02880"
     CDS             13219..13770
                     /gene="rsmD"
                     /locus_tag="VFO53_02880"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET8875248.1"
                     /translation="MPAPHRVRIIGGRHRGRQVAVPPVAGVRPTPDRVRETLFNWLGQ
                     DLTGATTLDLYAGTGVLSLEAQSRGAERSVAIDRNPALIRALRNAAEALGIAAIETHV
                     GEALPYLSGESRTFDVVFVDPPFADDPWDALFALLRPRLALDGRLYAEAGRALTPPPQ
                     FELLRHARAGHVHYHLLARTDRR"
     gene            13783..14268
                     /gene="coaD"
                     /locus_tag="VFO53_02885"
     CDS             13783..14268
                     /gene="coaD"
                     /locus_tag="VFO53_02885"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET8875249.1"
                     /translation="MKVVYPGTFDPFTRGHEDLVRRAGRLFDTVVVAVADSATKKPFF
                     STGERIAMAREVLRPFPNVEVIGFSSLLMDFVVGQGAQAIVRGLRAVSDFEYEFQMAG
                     MNRHLDPDVETLFLTPGEKYMFISATIVREIARFGGDVTKFVHPHVGECLRRKVAADV
                     R"
     gene            14278..14535
                     /locus_tag="VFO53_02890"
     CDS             14278..14535
                     /locus_tag="VFO53_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HET8875250.1"
                     /translation="MALIITDECINCDVCEPECPNQAISQGAEIYEIDPRKCTECVGH
                     FDKPQCREVCPVDCIPVNPDFIETPEALMRKYEALTAAKPQ"
     gene            complement(14644..>17217)
                     /locus_tag="VFO53_02895"
     CDS             complement(14644..>17217)
                     /locus_tag="VFO53_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET8875251.1"
                     /translation="RRKYSRMPELPEVETTRRGIAPHVIGRRVARIDVYDSRLRWPVP
                     GDLDACMRGRTIDRVDRRSKYLLFHAGEAALLVHLGMTGTLRVFREPPPRRTTRSRRA
                     AILPELPAPPTQPASRTAVPLRGMCRRSAARACDAQPMGGADRLPNAHRFASDIGGGI
                     GALPSRRTRPLIRHELCFPAADRLRVTIEAFLPQPRTRGGMNPSRDLAARFEAYADWR
                     RRLSAGVSSLFEWLTQQDLADAQVEVKVQRILERLQQDKLVVAFVAEFSRGKSELINA
                     IFFADFGQRLLPSSAGRTTMCPTELLHDPSRPPSIRLLPIETRLNEATVTEFKTYADE
                     WVTFPLDLGAADRMAEALARVSQVKRVPIALARKYGLFSERDEDILSALERGDEGSVD
                     IPCWRHAIINFPHPLLQQGLVILDTPGLNAIGTEPELTLNLLPNAHAILFLLAADAGV
                     TKTDLDVWNDHLAGDDDGMKSARLVILNKIDGLWDDLKEEVEINAEIDRQVDASATLL
                     GISPAQVFGVSAQKALLAKVNGDDALLARSRLPVLENSLSRKLIPAKRELVGAATQAE
                     LRGLTSSLRTILDARAASIGEQLVELIALRGKNQDVVEHMMARVQEEKALFERGLARY
                     TALRTVFTQHTNDLFDVIGLSALRQNATKTRQRIEDSPFTKGVRDAMGEFFGRLRTDF
                     DQAGRQAAEIHDLMRAMYTRFAHEHGIEIYVPPQFSVLKYLKEIERMERAYNTHFNTL
                     WNMVSKAKYPLMKQFFETVASRARHIYKVANRDVEGWLKAVMSPLETQVREHHIQLRR
                     RLDSIRRIHRASDELENRIMELEQQREALDTQRQGLREQLSTVDMIIEAPDALPLAAN
                     G"
BASE COUNT         2642 a         5858 c         5880 g         2837 t
ORIGIN      
        1 atcgaccccg ggatcgcgaa gacgtcgcgg ccctgctcac cggcgagacg cgccgtgatg
       61 agcgagccgg acgaaagcgt cgcctcgacc acgagcacgc cgcgagcgag gccgctgatc
      121 aggcggttgc gttgcggaaa attctccttg cgggcgggcg tgcccggcac gtattccgac
      181 accagcgcgc cgttgcgtgc gatgcgcgcc gcgaggtcgc ggtttcgcgc cggatacact
      241 cgatcgagcc cggtgcccac cacggcgagc gtcgatcccg tcgtttcgag tgcgccttcg
      301 tgcgccgcgg catcgatgcc gacggcgagg cccgacacga tggtgagcgc cgcgctgccg
      361 agggcatgcg caaattcacg cgcggtttcg cggccttgag gcgtcgcgtg acggctgccg
      421 acgatggcaa aggccgggtg cgccagcagc tcgcgccgcc ccatgtggaa cagcaccggg
      481 ggcgcatggc cgagctcgag cagcgccggc ggataatcgc cgtcgtccca cgcgatcagg
      541 tgatgttccg gatgatcgag ccacgcctgc gtgtgctcga ggagttcgcg ttcgacggat
      601 gcgagcgtgc gctcggcgac ggcgggcggg acgaccgagg cgagctgccg gtgcgacgcg
      661 gcgagcgccg cctcggggct accgaactgg cgcagcaacg cggcgacggc gcgctgcggc
      721 agcgtcttgt gcgcgagcgt tgcccaggcg agggtgcgag aatcgagcgc caccgctcaa
      781 agcctcgcga aagacgacag tgtgcgctta cacgaagcgg gcgcgcgcgc cgagtgaggc
      841 cagggtcatc atggcgtgcg cacgaaatcg ccggtacgca ccgcaccgct ggagttcagc
      901 acgagcgcgt acgacacgcg atcgaacacg cggaagacca tgaggatgcc cgtgcgctcg
      961 tcgggcacgt gcaggtaggc gggcggcgtg tagaacgtcg tctggtcgag gttggggaac
     1021 tggacggtgg gttcggtctt gttgcgccgc ggatcgcgga tcggcgcgac aacgcgatag
     1081 atcgccagca cgttgccgac ttcgagaccg tcgcgcgcgc ccttgtcgag cgtgacgacg
     1141 taaccgcgac cggtttcctg cacgtcgcgc gcgagcttca ggatgcggcc gtcgatgacg
     1201 tgatcgggcg cgtgcgggat gtagttctgc acgatctcgc gcgtggccgg gagcaggcgg
     1261 tcgccgatca cgatttcttc ggtcgccgat tcgatccggg cggtcgagac gtcggcgaag
     1321 cgctcgacgc gcacggtgcc gaggaaacgg ttttcgaagc cgagcgtttc gccggcgctg
     1381 ttgacgagcc gctcgcccgg acggtagacg aaccacaggt cgccgccctt cggatcgagg
     1441 ccgaccgcgt agacgatatc ctgctgtccg cggatgacgc gctcgtcgcg gccggcgacg
     1501 atctctgcag cgtcgcccag gccctcgggg ccggtgacga gcggacgcga caggaacggc
     1561 tcgatatcgc ccggcgggat cgcggggatg gcttcggggg cgagcggcga aaccctgatt
     1621 tccggcgaga gtcttaccgt attgcgcgcg agatcgaggc gcgggctgcc gtcgacgacc
     1681 gtgagccgga tgacgtcgcc agggtagatc cggtgcgggt tcctgatctc ctcgcggttc
     1741 atccgccaca cttcgggcca gcgccacggc tccttgagga agcggccggc gatgccccac
     1801 agcgtgtcgc ccttttgcac ggtgtacgtg gacggtgcat tatcggcgat gccgacggtc
     1861 tgcgccgata ccggcgccgc gagcgccaga atggagagga cgccgcacag ggcggcgatg
     1921 cgtgttgcca tggtaaactg ttgacgcata gggcctcttt ccggtcgggc ggacttgcta
     1981 acgtcctaaa ccgccacaaa ataaggactt gaagtcgatt cttatctaag agcttgtatt
     2041 ttgcaagcca ataacgcaat ggccctgctt ccgatcctcg aatatcccga tccgcgtctc
     2101 aaaaaggttg caaccccggt tgccgccgtg accgcggacg tccgcaggct cgtgcgcgac
     2161 atggccgaca cgatgtacgc cgcgcccggg gtgggcctcg cggccacgca ggtcgacgtg
     2221 cacaagcggg tgatcgtcat cgacatctcc gagcacaagg acgagctgcg cgtattcatc
     2281 aaccccgagc tgctttcggc cgacggcgag gccgaatgcg aggaaggctg cctgtcggtt
     2341 cccggctact acgacaaggt gacccgggcc gcccgcatcc gcgtgcgtgc gcaggacgag
     2401 catggcgaaa cgttcgagct cgacgccgag gggctgctcg cggtgtgcat tcagcacgaa
     2461 atggaccatc tgcagggcaa ggttttcgtc gaatacctgt cgccgctcaa gcgtgcgcgc
     2521 ctcgccgcga agttgcgcaa gaaacagcgc ctcgcgggct gatcgctgca tcgtccatgc
     2581 gcgtgggttt tgccggaacg ccggcgttcg ccgcccgcgc gctggcggcc gtccacgcca
     2641 agggcttcac gataccggtc gtcctgacgc agcccgatcg gcctttcggc cgaggacttg
     2701 cgattgccgc gtcaccggtc aagcgctacg ccgccgacca cgcgctgccg ctgcgccagc
     2761 caccgaccct caagctcgac accgtgcagg cagcgcttcg cgacattccg atcgacgtgc
     2821 tggtggttgc cgcgtacggc ctgattctgc cgcagccggt cctcgactgg ccgcgtcacg
     2881 gctgcctgaa cgtccacgcg tcgctgctgc cgcgctggcg cggcgcggcg ccgatcgcac
     2941 gcgccgtcga ggcgggcgac gcgatgagcg ggatcacgat catgcaaatg aacgccggcc
     3001 tcgacaccgg tgcggtcgtg accgagcgag cgctcgcgct cggtgcgcgc gagacgaccg
     3061 gaacgctcac cggccggctg gcgatcctcg gcgcatcgat gatcgtcgat gcgctgtgca
     3121 cgctcgagcg cgacggccgc ctgcaggcca cgccgcaacc ctctgacggc gccacgtacg
     3181 ccgcgaagat cggtcgcgcc gacacgctga ccgactggac gcaggacgcc gaagcgctcg
     3241 atcggaggat ccgtgcgctt gcgccgtcgc ccggcgcgct tgccggctgg cgcgggaagc
     3301 cggtcaagat tcacgcggca gtgccggtcg aggcgaaggt cgccgcggac gccggtacgg
     3361 tcgtcgcact cggcgccgac ggcatcgacg tcgcttgcgg cagcggcgtg ctgcggctga
     3421 cccaactgca gccggcgggc ggccgctcca tgtccgcgca ggcattcgcc gcgggccacc
     3481 gcgtggcggc cggcgagcgg ttcgagccgg gccgataggc acgaatgaaa caggagcaga
     3541 cgcaggcggc actggccgtc atgcgcgttt tcgacggcgc gcaactgcgc gacgcgctgg
     3601 cgctcgtcga cgacggcagc gcactgcgcg ggcgcacgct cgttcaggag atcgcgtacg
     3661 gcacgctgcg ccactggggc gcgctcgacg cgatcgtcgg gcgtctcgca cgaaagccca
     3721 tcgccgatcc gctgctgcgc acgctggttg cggtcgcgct ctatcagctc gagcacacgc
     3781 gcgcgccgcc gttcgcggtg gtcgatcggg cggtcgcggc cgcgggcgac gcggtgcgtc
     3841 cggcggcgaa ggcactcgtc aacgcgctgc tgcgccgcta tctgcgcgag cgcgacgagg
     3901 tccaatgcgc catcgcgcaa agcgacgtgg cgcgctggtc ctatccgcaa tggtggatcg
     3961 agcgggtgcg ccgcgattac ccggcgcaat ggcaggcgat actcgaggcg ggcaacgcgc
     4021 gcgcgccact cacgcttcgc gttaaccggc gggtgacgtc gcgcgaggcg ctcgccgagc
     4081 gcttcgcaga cgcgggcatg gccgtcgagc cggtcgccga atgcgcactg atcgttggcg
     4141 tgccgcggcc cgtgcgcgag ttgccgggtt tcgacgacgg cgcattcgcc gtgcaggatc
     4201 tcgccgcaca gctcgcggcg ccgctcctcg acgtgcacga cggaatgcgc gtgctggacg
     4261 cctgcgccgc ccccggcggc aagacgaccc acctcctcga gagcgccgac gccgacgtgc
     4321 tcgccctcga catcgacacg cgccgccttg cgctgatccg tgaaaacgtg gagcggttgc
     4381 ggctcggcgc gcaccggctg cgcctcgtcg agggcgacgc cggcaacccg gcggcgtggt
     4441 gggattcgcg cccgttcgat cgcatcctgc tcgatgtgcc ctgcacggca tcgggcgtgg
     4501 tgcggcgcca tcccgacggc aagtggcgcc accgcgccgg cgatgtcgac cggttcgcgc
     4561 gcgagcaggc gcggctcctc gcggccgcgt ggccgcttct ggcccccggc ggcaagctgc
     4621 tctatgccac ctgctcggta ttcgtcgctg agaacggggc acaggtcgcg aaattcgttg
     4681 cacagcactc cgatgcgttg cgcgaaaccc tcaatttccc gagcgcaacg ggggctcggg
     4741 acggacaact cttgccatcg ccggaagccg cgagccacaa tcaggacggg tttttctacg
     4801 cgctgcttcg caagcgctga tgtggtgcct cggccacgca gcggttctgc gcggcgcatc
     4861 gccacgcccc ggcctcgggg cgcgtcccgc cggcatgccg tcatccctac gcaccgaccg
     4921 cgcgcccgcc acgccacggc gccgcttcct gatcgcgctg atcgcgctga tcggcagcgc
     4981 atggctcgcg cagccggtcg cggccgacga gattccggtg aagtcggcgt cgcttcgcgt
     5041 cgacgaggcc gacgtgctgc tctcggcgga gttcgacctt tcgctcaccc cggcgctcga
     5101 gcaggcgctc gacaagggtg tcgcgctcta tttcacgatc gaattcgagc tgttgcgcac
     5161 gcgcgccttc tggtttcccg aaaagctggc cgactggtcg atcacctacc gcgtctcgta
     5221 cagtccgctc acgcgccagt atcgcgtggc gagcgggccg ctcgggcagg cgttcgaatc
     5281 gctcgacgac gtcgagcgat tcatcgggca cgttgcgtcg cggcccgtcg cgcaggcgag
     5341 cgatctcgtg aaaggggcct cgtacgaggc cgcggtacgg atgaagctcg acgtcaatca
     5401 gctcccgaag ccgttccagt tgaacgcgct cgcctcgcgc gagtggcagc tcgcctccga
     5461 ctggtatcgc ttcggcttca cgccatcatg accgcgcgcg catcttgaac atccttgcct
     5521 cgccgacgct gcgctgggtg acgctcgggt tcgcctgcct ctcggcgatc gcgctgttcc
     5581 tgctcgcgag cgcgacggcg aacaccgatt ttttcgccgg ccactacgac gcgctcgtcg
     5641 tcatcaacgg tgcactcgtg gcgctgttga tgctcgtcgt gggcggccag cttctgcagc
     5701 tctggcgcag ccttcgcgcc ggcgtgttcg gatcgcgcct tgcgttgcgg ctcgtgctgc
     5761 tgttttcgct cgtcgctgtc cttccgggcg cgctcgtcta tgccgtctcg gtgcagttcc
     5821 tcggccgcag catcgagagc tggttcgacg tgcgcgtcga tcgcgcgctc gacggcggca
     5881 tcaatctcgg ccgcagcgcg ctcgactacc tgctgaagga aacgaccaac cgcgccacgc
     5941 tcatttccgg cgcaatcgcc gacgcgcaag gcgcacccgg tgcggcgctc tcccgtgcgg
     6001 cggagcaggt cggcgtctac gaggcggcgc tgttcgcgcc gtccggcagc gtcgtcgccg
     6061 ttgcgggtct cggcggcggc atgacgcccg aggcgctccc cggcgacgcg cttcgccgtg
     6121 cacggctgca gcagacgtac gcgaaggtcg agcagcagca ggatggcggc ctgatgcttc
     6181 gcgtcgtcgt tccggtcaac agcggcgatc gaatcgagcc gctgcgcgtg ttgcaggtga
     6241 tcgagcgcgt gccgcgcacc ctcgcgcagg atctcgagaa ggtccaggcg ggcgcccgcg
     6301 actaccagga aatttcattc tcgcgcggcg cgctgaagcg gctctaccag ctgacgctga
     6361 cattgacgct gctgctcgca ctcacgtccg cgctcggtct ggcgctcgtg ctctccgagc
     6421 gcttcgcatc gccgctcgga ttgctcgccc aggggacccg cgcggtggcg gaaggcgatt
     6481 tcacgcggcg ccatccggtg acatcccgcg acgagctcgg cgtgctcacc gaatcgttca
     6541 atacgatgac ggcgcagctc gccgacgcga aggcgaagac cgaggagtcg cagcgggcga
     6601 cggagacgac gcgcgcctac ctcgaaagcg tgctgtcgaa cctgtcgtcg ggggtgctcg
     6661 cgttcgacga tcggtatcga ctgcggaccg cgaatccgag cgccgccgtc atcctgcagc
     6721 agccgttcgc cgagctcgcc ggcgttccgc tcgccgagtg gggcggacgg cttcccgcgc
     6781 tcgcgccgtt cggcgagctc gtcgccgaag cgtttcgctc ggcacgcgag ggtcactggc
     6841 agcgcgaagc gtcgctcggc gttgccgcgc agacgcgcac gctgctcctg cgcggcacgc
     6901 ggctgcccgg cgcgccggtc cccggctatg tcgtcgtgtt cgacgatgtc tcggagctct
     6961 tgcaggcgca gcgcgacgcg gcgtggtcgg aagtcgcccg gcgcctcgcc cacgaaatca
     7021 agaatccgct gacgccgatc cagctctccg ccgaacggct cacgctgaag ctcggcccgc
     7081 acctcgacga agcggaccgg gaaacgctgc gccgtggcac gcagacgatc gtgtcgcagg
     7141 tcgcggcgat gaagcacatg gtcgacgatt tcgcgatcta tgcgcgcaag gcccgcccgg
     7201 gccagatgca ggcggtcgac ctcgcgtccc tcttgctcga cgtgctcgct ctgtacgaca
     7261 atctgcgccc gcatgtcgcc ctcgcgctac ccgacgcgcc ggtcatgatt cagggcgagc
     7321 cgacccggct gcgccaggtg atccacaatc tgctccagaa tgccgtcgat gcacaatcgg
     7381 ggcaggacga gtcgcagatc gacattgcgc tgtcggtcgg cgacggggag gcgatactcg
     7441 cgtttagcga tcgcggcccc gggttttccg aaaacgtgct cgcgcatccg ttcgagccgt
     7501 atgtcaccac gaaagcgaaa ggcaccggtt tgggactggc catcgtgaag aagatcgtcg
     7561 acgagcacgc gggacgcgtg acgctcgaga acgtcgcacc gcgaggcgcg cgcgtgacgc
     7621 tgcatctgcg cgcgctcaag tcggttgccg cggcatgaat ccggcgcgtg cggcgcaggg
     7681 ccgcgaaatc ctggtcgtcg acgacgaggt cggcatccgc gaactgctgt cggaaatcct
     7741 gcaggacgag ggctaccgcg tggccgtcgc cgagaatgcg ggcgaggcgc gtgcctatcg
     7801 gcagcgccag cagccggcgc tggtgctgct cgacatatgg atgcccgaca ctgacggcgt
     7861 gacgctgctg cgcgaatggg gatcgagctc gctgctgacg atgccggtca tcatgatgtc
     7921 gggccacggc acgatcgaaa ccgcggtcga ggccacgcgc atcggcgcgt tcgactttct
     7981 cgaaaagccg atcggcctct ccaaactgct gtcgacgatc gcgcgcgcgt tcaagtcggc
     8041 ggcggcaaag gagccacggc gaatttcgct cgcagcgctt ggcagcggcc ccgccattca
     8101 cgacgccgaa cgcgcgctcg aggcgttgct cgccgcacgg cgcgcggtgc tgatcgccgg
     8161 cgagccgggc acgggccacg atgttgcggc gcgctcgctg caggttccgg gcgcgccgtt
     8221 cgtagcactc gccaatggag cgcgactcgc gaacaatccg ctcgcgttga tcgaggaggc
     8281 gcgcgagggc gtgctgtact gtcccgagat cggccagtat tcgcgcgcgg agcagaaggg
     8341 gctcgcgttc ctgctgccga agctcgacaa gcacaacatc gtgctcgtcg cgacgagcgc
     8401 cgagccgctc gcgaacctcg cgacggaagg caagttcgac gcagggttgc tgtcgcagct
     8461 ttccgccggc gcgatcgtgc tgccgccgct gcgcagccgg cgcgacgaca tcccggcatt
     8521 gatcgagcgc ttctggcgct cgggcggcgc cgaggagcgc gcaacgccgc tgctcgcgac
     8581 gctgtcgccc gcggcgctca cgatcctgtc cagcgcgtac tggcccggca acctcgatca
     8641 tctgcaaagc gtcgtggcga acatgcagat cgtgcgcggc gagatcagcg ccgatcacgt
     8701 gaagcgcctg cttggtgaag ccgcgtcgcc gaaccaggtc gtggcacccg agatcacgac
     8761 gcgcttcttc gagttgccgc tgcgcgaggc gcgcgaggcg ttcgagcgca tctacttcga
     8821 gcagctgctc ggccgcgagc agagcaacat gagcaaggtc gcggagcgcg ccgggctcga
     8881 gcggacgcac ctctatcgca agctcaagca actcaacatc cgcttctcgc ggcgcaccga
     8941 cgacagcagc gccgtctcgt gacggcgcga gagccgaaag cgaccttgag ttcggccgtc
     9001 gcatccgctc gcccgcccgc ccgcatgctg ccgctcgcgc tcggtgcggt cggcgtcgtc
     9061 tacggcgaca tcggcacgag cccgctgtac acgctgcgca cggcgttcac cggctcgtac
     9121 gggctttcgc tcacgcccga caacgtcctc ggcgtcgtct cggtcatctt ctgggcgctc
     9181 ctgatcgtcg tgacgctgaa gtacatcacg ctgatcatgc gggccgacaa ccggggagaa
     9241 ggaggcatcc tcgcgctgac ggcgctcgtg tcgcgcggca tcgagagtcg cgatcggctt
     9301 cgctggtggc tggtcggcct cggcatcttc ggcgcggcga tgttctacgg cgatggaatg
     9361 atcacgccgg cgatcagcgt gctcggcgcc gtcgaggggt tgcgcgtcat cgcaccggcg
     9421 atgcatccgt tcatcgtgcc cgcgacgatc gtcatcctcg tggtgctgtt cgccgcgcag
     9481 cgcaagggca cggcgggcct cggggcggtg ttcggcccgg tgatggtgat ctggttcatc
     9541 gcgctggcgc tgctcggcgc gctttccatc gggcgcaatc tcgccgtgct cgaagcgctc
     9601 gagcccgtct atgcgttccg tttcatcgcc ggcaatcccg gcaccgcgtt cgtggcgttc
     9661 ggcgcggtgg tgctcgccgt gacggggacc gaggcgctgt atgccgacat ggggcacttc
     9721 ggccgcagcc cgattcgccg cgcgtggctg ttcttcgtgt ttcccgcgct cctgctgaat
     9781 tacttcgggc aggcggcgct cgtgctcgac aacccggcgg cgatcgagaa cccgttttac
     9841 ctgctcgccc ccgaatgggg acgggtgccg ctgctgctgc tcgcgacggc ggcggcgatc
     9901 atcgcatcgc aggcggtgat ctcgggcgcc ttctcgctca cccgagcggc catccagatg
     9961 ggctactgtc cgcggctcac gatcatgcac acgtccgagc gcgcgatcgg ccagatctac
    10021 atcccgttca tcaactggat gctgctcgcg gccatcatcg ggctcgtcgt cggattcggc
    10081 aactcggaca atcttgccgg cgcctacggc atcgcggtga cgatggcgat gctgatcgat
    10141 tcgatcctga tcttcgtcgt catgcggcgg ctctggaact ggccggtccc catagcgatc
    10201 ctgatcgcga cgccgctcct gctgatcgat ctcacgtttc tcgcgtcgaa tgcgctgaag
    10261 attcccgagg gcggatggtt tccgctgctg atcggcgccg tcgtgttcac gctgctcacg
    10321 acgtggaagc gcgggcgctc gctcctgatg cgccgcctcg ccgacgacgc gatgccgctc
    10381 gagcttttca tccagagcat cgaggcgtcg ccgccgaccc gcgtgccggg aacggcggtg
    10441 ttcctcacgt cgacgcagaa ccgcgttccg catgcgctgc tgcacaacct caagcacaac
    10501 aaggtgctgc acgagcgggt cgtgttcctg accatcgtca ccgaggacat tccgtacgtg
    10561 cccgacgagg agcgctacga gatcaagccg ctcggctgcg acttcttccg catggtcgcg
    10621 aactacggct tcaaggacga ccccgacgtt ccggagctgc tcgaggactg cggccggcgc
    10681 ggcttcgtgt tcgacatgat ggagacgtcg ttcttcgtgt cgcgcgaaac gctgatcgcg
    10741 accgtcacgc ccgggatggc gctatggcgg gagaagctgt tcgtgtcgat gtcgaagaac
    10801 gcgacgaagg cgtcggaatt cttccaggtg ccgacgaacc gcgtcgtgga gctcggcacg
    10861 caggtggagc tgtagcgagc gcggcgcgca ggtggcgaaa aaacgccggc acgaagccgg
    10921 cgtccgttcg ccgcgaatgg cgcgttactg aagcgtcacc atctcggagc gcatcgtctt
    10981 cagcgccttg ctctcgatct ggcgaatgcg ctccgccgac acgccatact cgttggccag
    11041 ttcctgcagc gtcgccgagt cttcttcccg cagccatcgc gcttcgatga tgcggcggct
    11101 ccgtgcgtcg agcttggaca tcgccgagcg caggccggcg gtgcggttcg ccgccatctg
    11161 cgcgcgctcg aggatctgcg acggctcgtc ctcggcatcg gaaaggtacg cgatcggcga
    11221 caccgagtcc tcgtcaccgg gcgtcggatc gagcgagacg tcgccgccgg agatccgctt
    11281 ctccatctcg aacacttcct cgggcttgac gccgaggtcg gccgcgatct tgttcgcctc
    11341 gtcgggggtc agcgccgccg acgaagtctt catgctgcgg aggttgaaaa acagcttgcg
    11401 ctgcgccttc gtcgtcgcga tcttgacgag gcgccagttg cgcagcacgt gctcgtggat
    11461 ttcggccctg atccagtgca gcgcaaacga caccaggcgg acaccgcgct ccggatcgaa
    11521 acggcgcacg gccttcatga gaccgatgtt gccttcctgg atcaggtcgg cctgcggcag
    11581 tccgtagccc aggtaattgc gcgacacggc gacgacgagc cgcaagtgcg acagcacgag
    11641 ctgccgcgcc gcatcgacgt cctcgtgctc gcgccagcgg cgcccgagct cggtctcctg
    11701 ctcggggctg agcagcggaa agcggttcac cgcctggatg tagtgatcga ggcttcccag
    11761 cgaggccggg gtgggaaacg cgagcgcact tccggacatt cagttctcct ttccatggcg
    11821 caccagaaaa tacttcgttg gagtgccggc gcgcctagtt tagcactcgt gtaatgagag
    11881 tgctaacagc cgggaaagtt cccgactgat ttagtcgact attgcgtggg aacgctcacc
    11941 gcgcaagccc gctgctaagg tggaccgccc ggggagatga cagttcggac ggctcgccgt
    12001 ttgatccggg cgggaccgag tgagcgcccg cttcgacgct aaggcagtct aggaaagcgc
    12061 ttcttcggcc ggcaaaagcg catcgacgaa ctcgtcggcg tcgaatggac gcagatcgtc
    12121 gacgccctcg cccacgccga tgaaccggag cggaatcggg tgctggcgcg caatggcggc
    12181 gacgatgccc cccttcgcgc tgccgtcgag cttggtcagg atcagcccgg tcaggcccac
    12241 cgccgcatcg aacgccttca cctgggcgag cgcgttctga ccggtgttgg cgtcgagcac
    12301 gagccacacc tcgtcgggcg cgccttcccg cgccttgccg agcacccgct tgatcttgcg
    12361 cagctcgtcc atcaggtgcg cctgcgtggg cagcctgccg gcggtatcgg ccaacacgac
    12421 gtcgaggccc cggctgctcg ccgcaccgac tgcatcgaac atgacggcac cgggatcgcc
    12481 gccggcctgc tggatgaccg cgatgttgtt gcgctcgccc cagacgacga gctgctcgcg
    12541 cgcggcagca cggaacgtgt cgcccgccgc gagcagcacc gagaggtgcc gctgttgcag
    12601 ccacttcgcg atcttgccga tggacgtcgt cttgcccgcg ccgttgacac cggccagcat
    12661 gatcacgaac gggcgcgccg cgccgaccgt caccggcgct tcgagcgtgc gcacgagatc
    12721 ggcaaatgca ccgcgcagca gcgcgcgcgg atcggcatcg gcgccggcct tccggtagcg
    12781 caaccgcaac tcgtccagca gatgctgcgt cgcagcgatc cccacatcgg ccatcaggag
    12841 cgccgattcg agcgcctcga gcgcctcgtc gtcgagcacg cgccggcgca aagcgccgga
    12901 taatgcgccc gacagcttct cgcgcgaaag gccgaggccc gacttcaacc gctcgcgcca
    12961 tgagcgcttc gcggcggaaa cgccggcggc agcttcggac ggcggcgcgc taggcacgcc
    13021 gggcaccccg gcgaccggct cggggggcgc cgattccgcg ggcgcggcag gctgcggggc
    13081 cggcttgcgt ttgaaaaggt cgaacatcgg gaaggcagcg ggcgctctac gccaggactt
    13141 tgacgcggcg cggggaaatt gggattctac ccggccccgc gcgggcgcca tgccgcgctt
    13201 gcatgatccc caccgcccat gccggcccct catcgcgtaa gaatcatcgg cggacgccac
    13261 cgcggccggc aagttgcggt cccccccgtt gccggtgtgc gtccaacccc cgatcgggtg
    13321 cgggagacgc ttttcaactg gctggggcag gacctgacgg gcgcgacgac gctcgacctg
    13381 tacgcgggta ctggcgtgct gagcctggaa gcgcagtcgc gcggcgccga gcgatcggtc
    13441 gcgatcgatc gcaatcccgc gttgatccgg gcgttgcgca acgcggcgga ggcgctcggc
    13501 atcgccgcca tcgagaccca cgtcggcgaa gcgcttccct acctctcggg cgaatcgcgg
    13561 acattcgacg tcgtgttcgt cgatccgccg ttcgcggacg acccctggga tgcgttgttc
    13621 gcgcttctgc ggccgcggct cgcgctcgac ggccgcctgt acgccgaagc gggccgagcg
    13681 ctgacgccgc cgccgcagtt cgagctattg cgccacgcgc gcgccgggca cgtgcattat
    13741 catctgcttg cgcggaccga ccggcgataa gccggcgcaa cgatgaaagt cgtctatccc
    13801 ggcaccttcg acccgttcac tcgcggccac gaggacctcg tccgccgcgc gggccgcctt
    13861 ttcgatacgg tcgtcgtcgc cgtcgcggac agcgcgacga agaagccgtt cttctcgacc
    13921 ggcgagcgca tcgcgatggc gcgcgaggtg ctgcggccgt tccccaatgt ggaggtcatc
    13981 gggttctcgt cgctcctgat ggacttcgtc gtcggccagg gggcgcaggc catcgtgcgc
    14041 ggcctccgcg cggtgtccga cttcgagtac gagtttcaga tggcgggaat gaaccgccat
    14101 ctcgaccccg acgtcgaaac gctgttcctg acgccgggcg agaaatacat gttcatctcg
    14161 gcgacgatcg tgcgcgagat cgcccgcttc ggcggcgacg tgacgaagtt cgtgcacccg
    14221 cacgtgggag agtgcctgcg acgcaaggtc gccgccgacg tgaggtagca tgggcggatg
    14281 gccctgatca tcaccgacga atgcatcaac tgcgacgtct gcgagcccga atgcccgaac
    14341 caggcgatct cgcagggcgc ggagatctac gagatcgacc cgcggaagtg caccgaatgc
    14401 gtcgggcact tcgacaagcc gcagtgccgc gaggtgtgcc cggtggactg catcccggtc
    14461 aaccccgatt tcatcgaaac gcccgaggcg ctcatgcgca agtacgaggc gctgacggcg
    14521 gcaaaaccgc aatagcgcgg cgacgacgcc aaaaaggggc caggctcgat ttccccccga
    14581 tttcccccgc tcggccgcgg tcgcggcatg aaaatcgagc ctggcccctt tatcaaccgc
    14641 ggcctacccg ttcgcggcga gcggcaaggc gtccggtgcc tcgatgatca tgtcgacggt
    14701 gctcaactgc tcgcgcagtc cctgccgctg cgtgtcgagc gcctcgcgct gctgctcgag
    14761 ctccatgatc cggttctcga gctcgtcgct cgcacggtgg atacggcgga tgctgtcgag
    14821 gcggcggcga agctggatgt gatgctcgcg cacctgcgtc tcgagcggcg acatcaccgc
    14881 cttcagccag ccctcgacgt cgcggttggc gaccttgtag atgtggcgcg cgcgtgaggc
    14941 gaccgtctcg aagaactgct tcatcagcgg gtacttcgcc ttgctcacca tgttccagag
    15001 cgtgttgaag tgcgtgttgt aggcgcgctc catccgctcg atttccttca ggtacttgag
    15061 cacggagaac tgcggcggca cgtagatctc gatgccgtgc tcgtgcgcga agcgcgtgta
    15121 catcgcgcgc atcaggtcgt ggatctcggc ggcctgccgg ccggcctggt cgaaatcggt
    15181 gcgcagccgc ccgaagaact cccccatggc gtcacgcacg cccttcgtga acgggctgtc
    15241 ctcgatgcgc tggcgcgtct tcgtggcgtt ctgccgcagc gccgagagac cgatgacgtc
    15301 gaagaggtcg ttcgtatgct gcgtgaacac ggtgcgcagc gccgtatagc gggcgagccc
    15361 gcgctcgaac agcgcctttt cctcctggac gcgcgccatc atgtgctcga cgacgtcctg
    15421 gttcttgccg cgcagcgcga tcagctcgac gagctgctcg ccgatcgacg cggcgcgtgc
    15481 gtcgaggatc gtgcgcagcg acgacgtgag accgcgaagc tcggcctggg tcgccgcgcc
    15541 caccagctcg cgcttggccg gaatgagctt gcgcgacagc gaattctcga ggaccggcag
    15601 gcggcttcgc gcaagcagcg catcatcgcc attcaccttg gcgagcaatg ccttctgcgc
    15661 cgacacgccg aacacctgcg cgggcgaaat gccgagcagc gtcgcactgg cgtcgacctg
    15721 ccggtcgatc tcggcgttga tctcgacctc ctccttgagg tcgtcccaca atccgtcgat
    15781 cttgttcagg atgacgagac gcgccgactt catcccgtcg tcgtcgcctg caagatgatc
    15841 gttccacacg tcgagatcgg tcttcgtgac gccggcatcg gcggcgagca ggaacaggat
    15901 cgcgtgcgca ttgggcagca gattgagcgt gagctcgggc tcggtgccga tcgcgttgag
    15961 tcccggcgtg tcgaggatca cgagaccctg ctgcagcagc gggtgcggaa aattgatgat
    16021 cgcgtggcgc cagcacggaa tgtcgacgct gccctcgtcg ccgcgctcga gcgccgacag
    16081 gatgtcctcg tcgcgctcgc tgaagagccc gtatttgcgt gcgagcgcga tcggcacgcg
    16141 ctttacctgc gacacgcgcg cgagcgcctc ggccatccgg tcggcggcgc cgagatcgag
    16201 cgggaacgtg acccactcgt cggcgtacgt cttgaattcg gtgaccgtcg cctcgttcag
    16261 ccgggtttcg atcggcagca gccggatcga cggcgggcgc gaggggtcgt gaagcagttc
    16321 ggtcgggcac atcgtggtgc gcccggccga cgacggcaaa agccgctgtc cgaaatcggc
    16381 gaagaagatc gcattgatga gctccgattt gccgcgcgag aactcggcga cgaacgcgac
    16441 gacgagcttg tcctgctgca gacgctcgag gatgcgctgc accttgacct cgacctgcgc
    16501 gtcggcgagg tcctgctgcg tcagccactc gaacagcgac gagacgccgg ccgacagcct
    16561 gcgccgccaa tcggcgtaag cctcgaaccg cgcggcgaga tcgcgcgaag gattcatgcc
    16621 gccgcgggta cggggctgtg ggaggaatgc ttcaatcgtg actcgcagcc gatcagcagc
    16681 cggaaagcac aactcgtgcc gtatgagcgg gcgagttcgc cgcgacggca acgcgccgat
    16741 gccgccgccg atgtcgctcg cgaatcggtg cgcgttcggc agccgatcgg cgccgcccat
    16801 cggctgcgca tcgcatgcgc gcgccgcgct ccgtctgcac atgccgcgca gtggcaccgc
    16861 cgtgcgtgac gcgggttgcg tcggcggcgc tggcaattcg ggcagaatcg ccgcacgccg
    16921 tgatcgcgtg gtgcgccgcg gaggcggctc gcggaaaacg cgcagcgtcc cggtcatgcc
    16981 gagatgcacg agcagcgcgg cttcgccggc gtggaacagc aggtatttgc tgcgccggtc
    17041 gacgcgatcg atcgtgcggc cgcgcatgca cgcgtcgaga tccccgggaa ccggccagcg
    17101 caggcgcgag tcgtacacgt cgatccgcgc cacgcgccgg ccgatcacgt gcggcgcgat
    17161 accgcgccgg gtcgtttcga cttccgggag ttcgggcatc ctcgagtatt tgcgacg
//
LOCUS       DASSVX010000062         4142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_169007, whole genome shotgun sequence.
ACCESSION   DASSVX010000062 DASSVX010000000
VERSION     DASSVX010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169007"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..329)
                     /locus_tag="VFO53_02900"
     CDS             complement(183..329)
                     /locus_tag="VFO53_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875252.1"
                     /translation="MSDLNHPLRALRAHRDYRLTPGEWLTCCAWALLLGICVVGETLE
                     TLFA"
     gene            606..878
                     /locus_tag="VFO53_02905"
     CDS             606..878
                     /locus_tag="VFO53_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875253.1"
                     /translation="MATLHKLNETDSGLRTTMSVAATLCVLGLATLVWLHDSPQISVA
                     AQPAPFVAVDSPAAGDTGTFGTGVPSAERVFGHATYAEPEVPIAQF"
     gene            complement(940..1602)
                     /locus_tag="VFO53_02910"
     CDS             complement(940..1602)
                     /locus_tag="VFO53_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8875254.1"
                     /translation="MSAARPMEPAVPVAELRFAIADDHPLMRAALASALSTLAPLVHF
                     VEATNHAEALRVVSQVPPHDLLLLDLHMPGTQGIDGVREVRECAPHVPVAIVSAEDDP
                     AAIRALLALGICGFIPKTDSTAVIASAVRLILAGGVYVPSHLVAFAPTCAINGTAAPA
                     LTGRQMEVVRLLGRGLSNKAIARELGVSEGTVKVHLLAVFRILDVRNRTAAVLAAQRV
                     LG"
     gene            1690..2835
                     /locus_tag="VFO53_02915"
     CDS             1690..2835
                     /locus_tag="VFO53_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875255.1"
                     /translation="MRSDGDNRWQSLAVERHDRLVSLGDGVGALARTADTLDRLHREL
                     GAPPAMDRWIDALDADLQQAKTIGPASTDPHLFRAALAVLGRRPAHPMLPLWHARALA
                     LVRRDDASADDALRAAKFSFEYAVRGGNFALAREIVTRARAHGADASVAMRRTWLEAE
                     ALEAWLAAEHGRARRAVAQAVALGAGYTAWEQGASAAISEGDLAEADRCLACMAASIE
                     RQRTQDIAHALFLGAARARLAGDNEGASERLDACFAQDATNVPAYFTTLWRLGRAHVD
                     VARQRHRPAGALLGVVIARAASHYWSFLHFSALLSRAWLRMREKRTAQATLDLRKALA
                     LAQAGAYRNCDPWWDREAIDEIGRFLRDALPEHPAWSALMQGRKVAA"
     gene            2832..>4142
                     /locus_tag="VFO53_02920"
     CDS             2832..>4142
                     /locus_tag="VFO53_02920"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HET8875256.1"
                     /translation="MSTLLRRVSALLAVMTLAVATVAMAQSAPQPPRLPPPVELQRAA
                     FAIGDYDGRFGDHSQHRVDLPHAWEVTDPGRTGDGWYTLSWTLDAVPTTPQAICATAL
                     TAPTEVYVNGELVGASGPLDGRRPRSYEQSRYIDLPAGLLRVGVNYIGVHMRAKAPNV
                     AGLGPILAGDATMLRERARGVLFVHTVGPAAVSIATLTVGVFILVLWLRRRDPGYLLF
                     AVAVMLWSVHTLFTLVPEPPLPQPHWGVVWTGTYLLFVAMLCLFCLRFASVRWPIYER
                     VVIVYAAATIPLLYIAQAFGELELASAGVRLGGIIMVVAALVAVARYAVRRWNVESAL
                     LLAAGTLSAAFAVHDWLASQDPLALRPVWLVPYAALAFLTLFGYLLTDRFVRALNESE
                     RLNVDLERRVEEKSAALSAQLSITQAAKDAAEAANRAKSSFLAAA"
BASE COUNT          639 a         1423 c         1408 g          672 t
ORIGIN      
        1 gatgctggcg cgcgagcgcc acgaacagcg tcagcgacgg cgtgcgcgag cgagcgcgat
       61 atcgcgagaa cgagcagtcg cagcagcgct cggcgacgac gcggcgcgtc gacgtgccgc
      121 catcgccaaa gccatcgagc gagcgcgcgt ccgccgacgg caagcgccct gaagccgacg
      181 cattacgcga agagcgtctc gagcgtttcc ccgacgacgc agatcccgag cagcaatgcc
      241 cacgcgcagc aggtcagcca ctcgcccggt gtgagccggt aatcgcgatg cgcgcgcaag
      301 gcgcggagtg gatggttcag gtcgctcatc atcgtagccc cctcggcgtg cgttggcgca
      361 cgaagcgatt ctcggacgtc ttctccgata gcgacatcgg gcatccggtg agcgcgactc
      421 tgtcgaacgg aacagcgcgt ggtgcatcct ggccaaacgg cgtacgccgt tcatcgcaat
      481 gcggcgacga ctcggccgtc cggttaccgg cgtctaatca caacgttgaa ttccggtgac
      541 gcctgattct gtcgacaatc cccatcgacg cgtatcaccg cacgcgttgc aaacgggaga
      601 tcgacatggc cacactgcac aagctgaacg aaaccgactc tggactgcgc accacgatga
      661 gcgtcgccgc gacgctttgc gtgctgggac tcgctaccct cgtctggctg cacgactccc
      721 cgcaaatcag cgtcgcagcg cagcctgcgc cgttcgtcgc agtggactcg cctgcggccg
      781 gcgacacggg cacgttcggc accggcgtgc cgtcggccga gcgcgtgttc ggccatgcga
      841 cgtacgccga gccggaagtg ccgatcgcgc agttctagga cggcttcatt tcctccaccc
      901 cggcaccggc tgcgccggtt tgccgggggt ttttcttctt cagccgagga cgcgctgcgc
      961 cgcaagcacc gcggcggtgc gattgcgaac gtcgaggatg cggaacacgg cgagcagatg
     1021 caccttgacc gtaccctcgc tcacgccgag ttcacgcgca atcgccttgt tcgagaggcc
     1081 tcgccccaac aggcgcacga cttccatctg ccgtccggtc agcgcaggcg ccgccgttcc
     1141 gttgatcgcg catgtcggcg cgaacgccac gagatgcgag ggcacgtaga caccgcccgc
     1201 gagaatcagc cgcacggcgc tcgcgattac ggccgtcgaa tcggtcttgg gtatgaaccc
     1261 gcagatgccg agcgccaaca gggcgcgaat cgccgccgga tcgtcctccg cggagacgat
     1321 ggcaaccggc acgtgcggcg cgcattcgcg cacctctcgc acgccatcga tgccttgagt
     1381 tcccggcatg tggagatcga gcagcagcag atcgtggggc ggcacttggc tcactacccg
     1441 cagcgcttcc gcgtgattcg tcgcctcgac gaaatggacc aggggtgcga gcgtcgaaag
     1501 cgcgctggcg agcgcggcgc gcatcagcgg atgatcgtcg gcgatcgcga agcgcaattc
     1561 agcaacgggt acggcaggct ccatcggacg ggcggcgctc acgtcgtttg tacgtcgcgt
     1621 gcattatagg cagaatgctc gccctggcag gatccgcgag catgcccgcc aaagggggag
     1681 cgcggccgca tgcgcagtga cggagacaac cgctggcagt cgctcgccgt cgagcgtcac
     1741 gatcgactcg tctcgctggg cgacggcgtc ggcgcgctcg cccgcacggc cgacaccctc
     1801 gatcggctgc atcgcgagct cggcgcgccg cccgctatgg accgctggat cgacgcgctc
     1861 gatgccgacc ttcagcaggc gaagacgatc ggtccggcga gtaccgaccc gcatctgttt
     1921 cgtgccgctc tcgccgtcct cggccggcgt cccgcgcatc cgatgcttcc gctatggcat
     1981 gcccgggcgc tggccctcgt gcggcgagat gatgcgagcg ccgatgatgc gcttcgagca
     2041 gcgaagttct cgttcgaata cgcggtgcgc ggcggcaact ttgcgctcgc acgggaaatc
     2101 gtgacgcgtg cgcgcgcgca cggcgcggac gcaagtgtgg cgatgcgccg cacctggctc
     2161 gaggcggagg ccctcgaagc gtggctggcc gccgagcacg gccgggcccg ccgcgccgtc
     2221 gcgcaggccg tggcgctcgg ggccggatac accgcgtggg agcaaggcgc gagcgccgcg
     2281 atatcggagg gcgaccttgc cgaggccgat cgctgtctag cctgcatggc agcgagcatc
     2341 gagcgccagc gaacccagga catcgcgcac gcgctgtttc tcggcgccgc gcgcgcgcgc
     2401 cttgcgggcg acaacgaggg cgcgagcgag cggctcgacg cctgcttcgc tcaagatgcg
     2461 accaacgttc ccgcctattt caccacgctc tggcggctcg ggcgggccca tgtcgacgtc
     2521 gcgcgccagc gccatcggcc cgcgggcgcc ctgctggggg tggtgatcgc tcgcgccgcg
     2581 agccactatt ggtcgttcct gcacttctcc gccctgctgt cgcgcgcctg gcttcggatg
     2641 cgcgagaaac gcaccgcgca agcgacgctc gacctgcgca aggcgctggc gctcgcgcaa
     2701 gccggcgcct atcgcaactg cgatccgtgg tgggatcgcg aggcgatcga cgagatcggg
     2761 cgcttcctgc gcgatgcatt gccagagcat cctgcctggt cggcactcat gcaagggcgg
     2821 aaggtggctg catgagcacg ctgcttcgac gcgtatcggc gctgctcgcg gtgatgacgc
     2881 tggccgtcgc gaccgtcgcg atggcccaaa gcgctccgca gccgccgcgt cttccgccac
     2941 cggtggagct gcagcgcgcg gcgttcgcga tcggcgacta cgacgggcgc ttcggcgacc
     3001 actcgcagca ccgcgtcgac ctgccccacg catgggaggt cacggacccg gggcggaccg
     3061 gcgacggctg gtacacgctg tcgtggacgc tcgatgccgt acccacgacg ccgcaagcga
     3121 tctgcgcgac ggcgcttacc gcgccgaccg aggtgtacgt gaacggcgaa ctggtcggtg
     3181 cgtcgggacc gctcgacgga cggcggccgc gaagttacga acagtcgcgc tacatcgact
     3241 tgccggcggg gcttctgcgc gtcggagtca actacatcgg cgtgcacatg cgcgcgaagg
     3301 ccccgaacgt cgcgggcctc ggtccgatcc ttgcgggcga tgccacgatg ctgcgcgaac
     3361 gggcgcgcgg agtgctgttc gttcacacgg tcggcccggc ggcggtgagc atcgcgacgc
     3421 tgaccgtcgg cgtgttcatc ttggtgctgt ggctgcggcg gcgcgatcca ggctatctgc
     3481 tgttcgccgt cgccgtaatg ctatggagcg tgcacacgct cttcacgctc gtacccgagc
     3541 ccccgctgcc gcagccccac tggggggtgg tctggacggg gacgtatctg ttgttcgtcg
     3601 cgatgctctg cctgttctgt ctacgcttcg cgagcgtccg ctggccgatc tatgaacggg
     3661 tcgtcatcgt gtatgcagcc gcgacgatac cgcttctgta catcgcacag gcgttcggtg
     3721 agctcgaact cgcgtccgcc ggcgttcgtc tcggcggcat catcatggtc gtcgctgcgc
     3781 tcgtcgccgt cgcccgttac gctgtccgcc gctggaacgt cgagagcgcg ttgctgctcg
     3841 cggcaggaac cctttcggcg gcattcgccg tgcatgactg gctcgcgtcg caagatccgc
     3901 tcgcgctgcg cccggtgtgg ctcgttccct acgcggctct cgcgtttctc acgctcttcg
     3961 gctatctgct gaccgatcgc ttcgtacgtg cgctgaacga aagcgaacgg ctcaacgtgg
     4021 acctcgaacg ccgcgtcgag gagaagagcg ctgcgctgtc ggcgcagctc agcattacac
     4081 aggcggccaa ggatgccgcg gaggcggcga accgtgcgaa gtcgagcttc ctcgcggcgg
     4141 cg
//
LOCUS       DASSVX010000063        10145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1152213, whole genome shotgun sequence.
ACCESSION   DASSVX010000063 DASSVX010000000
VERSION     DASSVX010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10145
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152213"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /gene="nagA"
                     /locus_tag="VFO53_02925"
     CDS             <1..1080
                     /gene="nagA"
                     /locus_tag="VFO53_02925"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET8875257.1"
                     /translation="VCGALEHAHGRIQRIDGEPIAEDRVRDEARAIVLPGFIDTHVHG
                     GGGSDTMEGGDAIVNIARMHARHGTTALLATTMTGPLHELEHALAALGPACRQRASGA
                     ARVLGVHLEGPYINPGKLGAQPDFVRQASIAEVLALHALAPIALITLAPELPGNLDII
                     AQLRGAGFRVQIGHTLATYEEGVAALERGAGGFTHLFNAMSGMHHRAPGIVGAALAHA
                     RYAEIIPDLLHVHAGALRAALRAIPCLFCVTDSTAAAGMPDGEYRLGSHRVRKCMGGV
                     RLADGTLAGSTLTMDQALRNLVLLGLPLAQASLRVSTHAADYLGVTDRGRIGSGAHAD
                     LVVVDRDLRLVRVIAEGEDIDLADA"
     gene            1073..2083
                     /locus_tag="VFO53_02930"
     CDS             1073..2083
                     /locus_tag="VFO53_02930"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET8875258.1"
                     /translation="MLDEAREAPDAIARLLAADAGRYAALAADLHEHPPRSLVTLARG
                     SSDHAAHYLAYLVMARLGHVVASLPMSLVTLYRSQLRCDGLVSFAFSQSGQSADLVLP
                     TRAFRERGARTVAFVNDTSSPLAGAAQWVLDLHAGPETSIAATKSHIAQLVAGARLVA
                     AWEGDTAMQRALDALPAVLEQAVAQDWTAAVNALSDVDRLYVVSRGLGLPIAMEAALK
                     LKEACGIQAEAYSGAEMRHGPMALVARDYPLLVFAPRGPMHDGLLAFADDMRSRGARV
                     VLAAPQGTRGSTLPITTAGVADLDPIAAIQSFYPVVEALARARGLDPDAPPHLSKVTL
                     TR"
     gene            2080..3393
                     /locus_tag="VFO53_02935"
     CDS             2080..3393
                     /locus_tag="VFO53_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8875259.1"
                     /translation="MRHAIALLLRIALLACVVTVLVACDSAAGPRKLHIEFWTMSLKP
                     KFTGYFDTLVAQYEAAHPGVELEWVDVPWDALQTKLTAAIVAGSAPALVQLNVPWAYD
                     YALYGALRPIDDLLGADRDRYTDGAIADLTFNGHVYGFPHYNSVNVMAYNTTLFRRAG
                     ITRVPTTRGEQLDAAKTIRARTGAYGFSPALGKIAGLMLEEGLPLVENGKAVFNSPAH
                     VAFVAKFADAYKAGGLLKDKLFSEDNYPASIDAYKSGRMAMLEAPPSALTRVRDDAAD
                     IYAVTDVAPVPIGARGIAAGGWLFHFAMPKGTTGEVAHEAAKFAKFLTDADNQVAFAK
                     LAGAFPTAKQAIADPHFRSLPLDAGAYEKAMAIGAARMEVVRTLYVSGIPGFERLNRR
                     LQDDVEAAIIGRRDIKSALDDAVAFWNAKLAPAATPTPGVASTLR"
     gene            3390..4328
                     /locus_tag="VFO53_02940"
     CDS             3390..4328
                     /locus_tag="VFO53_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8875260.1"
                     /translation="MSRVVEGAGQVDALHRDESNALRLHLPSRRALRPWLFLAPALVL
                     LGVFTFWPVLFGSYLAFTEYNFLTPPRFVGLANFEDLLADPVFLVSIRNSLLYLAIVP
                     AIQLGAIVLAVLVNNALPGIRFFRALFYTPVVTSVSVVGIMWGWMYNEQGLVNAVLER
                     LHVIGEPIGWLTDDRLALFSLMLVTMWRGLGWYMVLYLAALQAIPKDLDEAAHLDGAT
                     RWQRFWRVTIPMIMPTILLCTVLSTLSAVKVLEEVLIMTKGGPVSSTYTGLYYAYDQG
                     IRAFNFPRALAASLVVSAFCIVLAWINFRVIRPRHR"
     gene            4334..5149
                     /locus_tag="VFO53_02945"
     CDS             4334..5149
                     /locus_tag="VFO53_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET8875261.1"
                     /translation="MRLELRRLVHYALLALIGLLCAFPFLWTLAVAIGTEGNVFNFPS
                     SFIPQRPSLANFVEVFRVIDIGRYYWNSIWITFWTVAWTILVCSLAAYPLARYRFRGQ
                     RLVFALILATLMLPTEVNFLVNFITAAHLRLVNTHLGVILPTLAGAIGIFMMKNAFEE
                     IPQDLLDAARVDGASELYVFLRIMLPLAGPQIGALVILTTVTTWNNFLWPSVVLTKSN
                     LMPLAVGILELSGTFGSSTRVIAAGAVLTIAPALIVFFFAQRFFMRGMEGAVK"
     gene            5146..6687
                     /locus_tag="VFO53_02950"
     CDS             5146..6687
                     /locus_tag="VFO53_02950"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HET8875262.1"
                     /translation="MSANGARLHPGELVMAGIRGRALDAAQAAFLRKQRIRAVVLFRD
                     NLGDENEVRALTAALRDVLGPRALIAIDQEGGSVIRATSLPQAPSAMALGAAHDAGLA
                     HDVGAAVARGIRSLGFNWNFAPVLDVNNNAANPVIAERSFSADPQEVIRLAGAWMRGA
                     MTERVACCIKHFPGHGDTHVDSHLELPRVDKPRAQLDTLELAPFRALCGEAPAVMTAH
                     IVYSQLDDRYPATLSRPILGNLLRGEWGYDGVVVTDSLVMKAIADRYGHARASVLAVR
                     AGADMVMALGTPMQQLDAIEAIRAAVQAGELDESGLLRARARLDALAARFPAAAGPYA
                     GEARAGDEALMRRAWARGLVALDGAKPPARATPIRVITQRFVPGDGVSEPGLSGAQVA
                     ALFAGFDDVDVVQVDDLAGLDPSLLPRDGRFGVLASNGRARYGSRARNLRPDLHLVLW
                     NPFQALDFAVPSLVTWGYAEGAREALHAWMAGSAAAPGCAPVPLRQHGFADVEHGRLG
                     HQIAR"
     gene            complement(6684..7424)
                     /locus_tag="VFO53_02955"
     CDS             complement(6684..7424)
                     /locus_tag="VFO53_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8875263.1"
                     /translation="MTSLAQILGNLDPNDSLPLYQQLQRGLREAIEKRILGPEDALPS
                     ERQLAEDLGVSRITVRKAIDGLAAEGLLVSRQGSGNFVSTRIEKNFAKLTSFSEDMRA
                     RGRTPRSVWLKRSEGTVTPEESLKLALSPGTLVYRFHRLRFADDEPMSIELATIVASS
                     LPSLDAVDASLYDALEHAGNRPVRALQRLHSMLLSAEQAKLLKAKPGDAGLFVERLGY
                     LRDGRAVELSQSYYRGDTYDFVAELTAF"
     gene            7773..>10145
                     /locus_tag="VFO53_02960"
     CDS             7773..>10145
                     /locus_tag="VFO53_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET8875264.1"
                     /translation="MKLRKTPIASAVALAWMSAVCSVHAQQTDAQAVDAQKVKPGDAT
                     AKTPTQDKAKDAKAPDARDAAAPAAPAKRAQETTIVAQAATPAPPAAPGAAVPLESIN
                     VIGIRQSLERSLEVKRNADSIVEVITAEDIGKLPDKNIADAVQRVPGVTISSAAGGEG
                     GFDENDRVSLRGTNPSLTQTLVNGHTIASGDWFVLDQVQTVGRSVSFSMLPSELVSQV
                     IVRKSATADLVEGGIAGAVDIITRKPLDFRKQFTGEASLQAVYADLPGKTDPQFNGLV
                     NWKNDADTMGIMLQGFYEKRHLRRDGQETLGYGTVSPTSALALAHPDLANAAYPTLIG
                     SSLFEQVRERSGGTIDVEIKPLTNLTVDFNAFYSHLEATNYNRNWMFFGSHVFPSGEI
                     PSSYTLTNGTITSAVFPNLGSPGNNHQYAIVDQILRPGAYSETSYYNLDAKYQATDRL
                     GFFGQVGYTRGLGKTPKQDVFEGDVFNTGADYTMHGIGGPVDVHFPGGNPSSFAGTSL
                     DWIFGASPASTEDKETYGKIDGTYQLGYGPWTSVKFGYRAAQHDRSTEQVGQGPNFAV
                     DPFNPANLPHWSGETYPSDFASGLGGDFPRNVWQLSPGELERWGDIYSNRDPAARRFW
                     SGEFSLREKVQAIYAMANLDGSNWSANFGVRAVQTKERVLVNVAIPTSICAVQAPCPQ
                     VPGAITTSAFGSFYQSPIEHTYTDVLPSANIKFDLTRNLVARFAAARTMARPDFSALG
                     GSISADDTTHTGNGGNPDLKPIRSTNLDAALEWYFQPRSLLSADVFYMDLS"
BASE COUNT         1599 a         3498 c         3371 g         1677 t
ORIGIN      
        1 gtctgcggag cgctcgagca cgcgcacggc cgcatacagc gcatcgacgg cgagccgatc
       61 gccgaggatc gcgtgcgcga cgaagcgcgg gccatcgtgc tgcccggttt catcgacacg
      121 cacgtgcatg gtggcggcgg cagcgacacg atggaaggcg gcgatgcgat cgtcaacatc
      181 gcgcgcatgc acgcacgcca cggcacgacc gcgctgctcg cgacgacgat gaccggaccg
      241 ctgcacgagc tcgagcatgc gctggcggca ctcggccccg cgtgccgcca gcgcgcgagc
      301 ggcgcggcgc gcgtgctcgg cgtgcacctc gagggcccgt acatcaatcc cgggaagctc
      361 ggcgcacagc ccgacttcgt gcgccaggcg tcgatcgccg aggttctcgc gctgcatgcg
      421 ctcgcgccga tcgcgctcat caccctcgca cccgaattgc cgggcaacct cgacatcatt
      481 gcgcaactgc gcggcgcagg ctttcgcgtg cagatcggcc acacgctggc cacgtacgag
      541 gaaggcgtgg cggcgctcga gcgtggcgcg ggcggcttca cgcatctttt caacgcgatg
      601 tcgggcatgc accatcgcgc cccgggcatc gtcggggcgg cactcgcgca tgcacgctac
      661 gcggagatca ttcccgacct gctgcacgtg cacgccggcg cgctgcgcgc ggcactgcgt
      721 gcaatcccct gtctcttttg cgtcaccgat tcgaccgccg ccgccggcat gcccgacggc
      781 gagtaccggc tgggctcgca ccgtgtgcgc aaatgcatgg gcggagtgcg tctcgcggac
      841 ggtaccctcg ccggcagcac gctgacgatg gaccaggcgc tgcgcaatct cgtgctgctc
      901 ggactgccgc tggcgcaggc gtcgctgcgc gtttcgacgc atgcggccga ctacctcggc
      961 gtaaccgacc gcggccgtat cggctccggc gcgcatgccg atctcgtggt cgtcgatcgt
     1021 gatctgcggc ttgtccgggt gatcgcggaa ggggaggaca ttgacctcgc ggatgcttga
     1081 cgaggcgcgc gaggcgccgg acgccatcgc gcggctcctc gcggccgatg ccggccgcta
     1141 tgccgcactg gccgccgatc tgcacgagca tccgccgcgg tcgctggtga cgctcgcacg
     1201 cggcagctcg gaccacgccg cgcactacct cgcctacctc gtgatggcgc ggctcggcca
     1261 tgtggtcgct tcgctgccga tgtcgctcgt cacgctctac cggtcgcagc ttcgctgcga
     1321 cggcctcgtg tcgttcgcgt tctcgcagtc cgggcaaagt gccgacctcg tcctgccgac
     1381 gcgtgccttt cgcgagcgcg gcgcgcgcac cgtcgcattc gtcaacgaca cctcatcgcc
     1441 gcttgccggc gccgcgcaat gggtgctgga cctacatgcc ggacccgaaa cgagcatcgc
     1501 cgccacgaag agccatattg cccagctcgt cgccggtgcg cggctcgtcg ccgcatggga
     1561 gggcgacacc gcgatgcagc gcgcgctcga cgcgctgccg gcggtgctcg agcaggccgt
     1621 tgcacaggat tggacggccg cggtgaatgc gctgtcggac gtcgaccggc tttacgtcgt
     1681 gagccgcgga ctcggattgc cgattgcgat ggaagcggcg ctcaagctca aggaagcatg
     1741 cggcatccag gccgaggcat actcgggcgc cgaaatgagg cacggaccga tggcgctcgt
     1801 tgcacgcgac tacccgctgc tcgtgttcgc gccgcgcggt ccgatgcacg acggcctgct
     1861 cgcattcgcc gacgacatgc gaagccgcgg tgcgcgcgtc gtcctcgccg caccgcaggg
     1921 gacgcgcggc agcacgttgc cgatcacgac cgccggcgtc gcggatctcg acccgatcgc
     1981 ggccatccag agcttctatc cggtggtcga ggcgctcgcg cgtgcgcgcg ggctcgaccc
     2041 cgacgcgccg ccacatctgt cgaaggtgac gttgacgcga tgagacacgc gatcgcactg
     2101 ctgttgcgca tcgcgctgct cgcatgcgtc gtcaccgtgc tcgtcgcgtg cgacagtgcc
     2161 gccggtccgc gcaagctgca catcgagttc tggacgatga gcctcaagcc gaaattcacc
     2221 ggctacttcg acacgctcgt cgcgcaatac gaagccgcgc atcccggcgt ggagctcgag
     2281 tgggtggacg tcccgtggga tgcgctgcag acgaagctga cggcggcgat cgtcgccggg
     2341 tcggcgccgg cactcgtgca gctcaacgtg ccgtgggcgt acgactacgc gctgtacggc
     2401 gcgctgcggc cgatcgacga cctgctcggc gccgatcgcg atcgctacac cgacggcgcg
     2461 atagcggacc tgacgttcaa cggccacgtc tacggcttcc cgcattacaa cagcgtcaac
     2521 gtgatggcgt acaacacgac gctcttccgg cgggcgggca tcacgcgcgt gccgacgacg
     2581 cgtggcgagc agctcgacgc cgcgaagacg atccgggcgc ggacgggtgc gtacggtttt
     2641 tcgcccgcgc tcggcaagat cgcgggcctc atgctcgagg aggggcttcc tctggtcgag
     2701 aacggcaagg cggtgttcaa ttcgccggcg cacgttgcgt tcgtcgccaa gttcgccgac
     2761 gcctacaagg cgggcgggct gctcaaggac aagctcttct ccgaggacaa ctatccggcg
     2821 tcgatcgatg cgtacaagtc gggtcgcatg gcgatgctcg aagcgccgcc gagcgcgctc
     2881 acccgtgtgc gcgacgacgc ggcagatatc tacgcggtaa ccgatgtcgc gccggtgccg
     2941 atcggcgcgc gcggcatcgc ggcgggcggc tggctgtttc atttcgcgat gccgaaaggc
     3001 acgaccggcg aggtggcgca cgaggcggcg aagttcgcga aattcctgac cgacgccgac
     3061 aaccaggttg ccttcgcgaa gcttgccggc gcgtttccga cggcgaagca ggcaatcgcc
     3121 gatccgcatt tccgcagcct cccgctggac gccggcgcgt acgaaaaggc aatggccatc
     3181 ggcgctgcgc gaatggaggt cgtgcgcacg ctctacgttt ccggcatccc gggcttcgag
     3241 cggctcaaca ggcggctgca ggacgacgtc gaggcggcga tcatcggccg gcgcgacatc
     3301 aagtcggcgc tcgacgacgc ggtcgcgttc tggaatgcca agctggcgcc ggccgccacg
     3361 ccgacgccag gcgtcgcgtc gacgctgcga tgagccgggt ggtcgaaggg gccgggcaag
     3421 tcgacgcgtt gcatcgcgac gaatcgaacg cgctgcgcct gcacctgcct tcgcgacgtg
     3481 cgctgcgccc ctggctgttt cttgcgcccg cgctcgtgct gctcggcgtg ttcacgttct
     3541 ggccggtgct tttcggcagc tacctcgcgt tcaccgagta caacttcctg acgccgccgc
     3601 gcttcgtcgg cctcgcgaac ttcgaggatc tgctcgccga cccggtgttt ctcgtcagca
     3661 tccgaaattc gcttctctac ctcgcgatcg taccggcgat ccagctcggt gcgatcgtgc
     3721 tcgccgtgct cgtgaacaac gcgctgcccg gcatccggtt tttccgcgcg ctgttctaca
     3781 cgccggtggt cacgtcggtg tcggtggtcg gcatcatgtg ggggtggatg tacaacgagc
     3841 aggggctcgt caacgcggtg ctcgagcgcc tgcacgtgat cggcgagccg atcggctggc
     3901 tcaccgacga tcgcctcgcg ctgttctcgc tgatgctggt caccatgtgg cgcggcctcg
     3961 gctggtacat ggtgctctac ctcgccgcgc tgcaggcgat cccgaaggac ctcgacgagg
     4021 cggcgcatct cgacggcgcg acgcgctggc aacgcttctg gcgggtcacc attccgatga
     4081 tcatgccgac gatcctgctc tgcacggtgc tctcgacgct gtcggccgtg aaggtgctcg
     4141 aggaggtgct gatcatgacg aagggcggtc cggtgagctc gacgtacacc ggcctctact
     4201 acgcgtacga ccagggcatt cgcgcgttca acttcccgcg cgcgctggcg gcgagcctcg
     4261 tcgtcagcgc tttctgcatc gtcctcgcct ggatcaactt tcgcgtgatc cgtccgcgcc
     4321 accgctgaac gccatgcgcc tcgagcttcg gcggcttgtg cattacgcgc tgctcgcgct
     4381 gatcgggctg ctctgcgcct tcccgttcct gtggacgctt gcggtggcga tcggcaccga
     4441 aggcaacgtg ttcaacttcc cgtcgtcgtt catcccgcag cggccctcgc tcgccaattt
     4501 cgtcgaggtg ttccgcgtga tcgatatcgg gcgctattac tggaattcga tctggatcac
     4561 gttctggacc gtcgcatgga cgatcctcgt ctgctcgctc gccgcgtacc ctctcgcgcg
     4621 ctaccgcttc cgcggccagc ggctcgtgtt cgcgctgatc ctcgccacgc tgatgctgcc
     4681 gacggaggtg aacttcctcg tcaacttcat cacggcagcg catctgcggc tcgttaacac
     4741 gcacctcggc gtgatcctgc cgacgctcgc cggtgccatc ggcatcttca tgatgaagaa
     4801 cgcgttcgag gaaataccgc aggacctgct cgacgccgcg cgcgtcgacg gcgcgtcgga
     4861 gctctacgtc ttcctgcgca tcatgctgcc gctcgccgga ccgcagatcg gcgcgctcgt
     4921 gatcctgacg acggtgacga cgtggaacaa cttcctgtgg ccgagcgtcg ttctgacgaa
     4981 gagcaacctg atgccgctcg ccgtgggcat cctcgagctc tccggcacgt tcggcagctc
     5041 gacgcgcgtg atcgcggccg gtgcggtgct gacgatcgcg ccggcgctga tcgtgttctt
     5101 cttcgcgcag cggttcttca tgcgagggat ggaaggcgcg gtcaagtgag cgcaaacggc
     5161 gcgcgcctgc atccgggcga gctcgtcatg gccggcattc gcggccgcgc gctcgatgcg
     5221 gcgcaggccg ccttcctgcg caagcaacga attcgcgcgg tcgtgctctt ccgcgacaac
     5281 ctcggcgacg agaacgaggt gcgtgcgctg accgcggcgc tgcgcgacgt gctcgggccg
     5341 cgcgcgctga tcgccatcga tcaggaaggt ggctcggtga tccgcgcgac gtcgctgccg
     5401 caggcgccgt cggcgatggc gctcggcgca gcgcacgatg ccgggcttgc gcacgacgtc
     5461 ggcgcggccg tcgcgcgcgg catccgcagc ctcggcttca actggaattt cgcgccggtg
     5521 ctcgacgtca acaacaacgc ggcgaatccg gtgatcgccg agcgcagctt ctcggccgac
     5581 ccgcaggaag tgatccggct ggcaggcgcg tggatgcgcg gcgcaatgac cgagcgcgtc
     5641 gcatgctgca taaagcactt tccgggacac ggcgacacgc acgtcgattc gcacctcgag
     5701 ctgccgcgcg tcgacaagcc gcgcgcgcaa ctcgacacgc tggagctcgc gccgtttcgc
     5761 gcgttgtgcg gcgaggcacc ggcggtgatg acggcgcaca tcgtgtactc gcagctcgat
     5821 gaccgctatc ccgccacgct gtcgcggccg atcctcggga acctgctgcg cggcgaatgg
     5881 ggctacgacg gcgtcgtcgt caccgactcg ctcgtcatga aggccatcgc cgatcgctac
     5941 gggcacgcac gcgcaagcgt gctggccgtg cgcgccggcg ccgacatggt gatggcgctc
     6001 ggcacaccca tgcagcagct cgacgcgatc gaggcgatac gcgcggcggt gcaagcgggc
     6061 gagctcgatg aaagcgggct ccttcgcgcg cgtgcccgcc tcgacgctct ggcggcgcgc
     6121 tttccggccg ctgccggtcc gtacgccggc gaagcgcgtg ctggcgacga agcgctgatg
     6181 cgccgcgcgt gggcgcgcgg actcgtcgca ctcgacggcg cgaaaccgcc cgctcgcgct
     6241 acgccgattc gcgtgatcac gcagcgcttc gtacccggcg acggtgtatc ggagcccggc
     6301 ctgtccggcg cgcaggtggc ggcgctattc gcaggcttcg acgacgtcga cgtcgtgcag
     6361 gtcgacgacc tcgccggcct cgacccgtcg cttctgccgc gcgacggccg cttcggcgtc
     6421 ctcgcctcga acggccgcgc gcgttacggt tcgcgtgcgc gcaacttgcg gcccgatctg
     6481 cacctggtgc tctggaatcc attccaggcg ctcgatttcg ccgtgccctc gctcgtcacg
     6541 tggggctacg ccgaaggcgc gcgcgaggcg cttcacgcgt ggatggcggg cagcgccgcg
     6601 gcgccagggt gtgcgcccgt gccgctgcgc cagcacggct tcgccgacgt cgagcacggt
     6661 cggctcggcc accaaatcgc acgttagaac gcggtcagct ccgccacgaa atcgtacgtg
     6721 tcgccgcgat agtacgactg cgacagttcg accgcgcggc cgtcacgcag gtagccgagc
     6781 cgctcgacga acaaaccggc gtcgcccggt ttcgccttga gcagtttcgc ctgctccgcg
     6841 ctgagcagca tcgaatgcag ccgctgcagt gcacgtaccg ggcggttgcc ggcgtgctcg
     6901 agcgcgtcgt acagcgaagc gtcgacggcg tcgagcgacg gcagcgacga cgcgacgatc
     6961 gtcgcgagct cgatcgacat cggctcgtcg tcggcaaagc gcagccggtg aaagcggtag
     7021 acgagcgtgc ccggcgacag cgcgagcttc agcgattcct cgggcgtgac cgttccttcc
     7081 gaacgcttga gccagacgct gcgcggcgtg cggccgcggg cacgcatgtc ctcggagaac
     7141 gacgtcagct tcgcgaagtt cttttcgatg cgtgtcgaga cgaaattgcc ggagccctga
     7201 cgactcacga gcaggccttc cgcggcgagc ccgtcgatcg ccttgcgcac ggtgatgcgg
     7261 gatacgccga ggtcctcggc aagctggcgc tcggacggca acgcgtcctc ggggccgagg
     7321 atccgcttct cgatcgcctc gcggagtccc cgctgcagct gctggtagag cggcaggctg
     7381 tcgttcgggt cgagattgcc gagaatctgg gcaagcgagg tcatggggca gcccttcccg
     7441 cgatggtcga ttgctcaacc agtttgggaa gaacaccgtg gtaacgcatt gatgggatga
     7501 gtcgcgtcat tcgcttataa taccaatgtc ataccaatcg cggcaaccgg cccgccgatt
     7561 ctcgtccgct cctgcactgc taacgtccgc accgtggtaa agacgcaacg catcgcatcg
     7621 tagcagtatc gcaaaattgg tagtgttttg ggttcaactg gtagtatagt ggcattgacg
     7681 ggcgcgacca acgcgccctg cttgcttgcg gcactcacgc ggtcgagttc cgtcgacgat
     7741 tcgcacgcaa cgccactcca gggggaagct tcatgaagct gaggaagaca ccaatcgcgt
     7801 cggcagtcgc actggcgtgg atgagcgccg tctgctcggt gcacgcgcag cagacggacg
     7861 cgcaagcagt cgatgcacag aaggtcaaac ccggcgacgc gaccgccaag acgccgacgc
     7921 aggacaaggc gaaggacgcc aaggcgcccg acgcacgcga tgccgctgcc ccggcggcgc
     7981 ccgcaaagcg cgcgcaggaa acgacgatcg tcgcgcaggc ggcgacaccc gcgccgcccg
     8041 ccgccccggg agcggcggtg ccgctcgaat cgatcaacgt gatcggcatt cggcaatcgc
     8101 tcgagcgctc gctcgaagtg aagcgcaacg ccgactccat cgtcgaagtc atcacggcgg
     8161 aggacatcgg gaagcttccc gacaagaaca ttgccgatgc ggtgcagcgg gtaccgggcg
     8221 tgacgatcag ttcggcggcg ggcggtgaag gcggcttcga cgagaacgac cgcgtcagct
     8281 tgcgcggcac caatccgagc cttacgcaaa cgctcgtcaa cggccacacg atcgcctcgg
     8341 gcgactggtt cgtgctcgac caggtgcaga cggtcgggcg cagcgtcagc ttctcgatgc
     8401 tgccgtccga gctcgtgagc caggtgatcg tgcgcaagag cgcaacggcc gatctggtcg
     8461 aaggcggcat cgcgggtgcg gtcgacatca tcacgcgcaa gccgctcgac ttccgcaagc
     8521 agttcaccgg cgaggcgtcg ctgcaggccg tgtatgccga tctgccgggc aagaccgatc
     8581 cgcagttcaa cggcctcgtc aactggaaga acgacgccga tacgatgggc atcatgctgc
     8641 agggcttcta cgagaagcgg cacctgcgtc gcgacggcca ggagaccctc ggctacggca
     8701 cggtctcgcc gaccagtgcg ctcgcgctcg cacatcccga tctggccaac gccgcgtatc
     8761 cgacgctgat cggctcgtcg ctgttcgagc aggtgcggga gcgctccggc ggcacgatcg
     8821 acgtcgagat caagccgctc accaatctca cggtcgactt caacgcgttc tactcgcatc
     8881 tcgaggccac caactacaac cgcaactgga tgttcttcgg ctcgcacgtt ttcccgagtg
     8941 gcgagattcc gtcgtcgtac acgctgacca acggcacgat cacgtcggcg gtgttcccga
     9001 acctgggctc gcccggcaac aatcatcaat acgccatcgt cgaccagatc ctccggcccg
     9061 gcgcgtattc cgagacctcg tactacaacc tcgacgcgaa gtaccaggcg accgatcgtc
     9121 tcggcttctt cggccaggtg ggctacacgc gcggcctcgg caagacgccg aagcaggatg
     9181 tgttcgaggg cgacgtattc aataccggcg ccgattacac gatgcacggc atcggcgggc
     9241 cggtcgacgt gcatttcccg ggcggcaacc cgtcgagctt cgccggcacg tcgctcgact
     9301 ggatcttcgg tgcgagcccc gcgtccaccg aggacaagga aacctacggc aagatcgacg
     9361 gcacctacca gctcggctac ggcccgtgga ccagcgtcaa gttcggctac cgcgcggccc
     9421 agcacgatcg cagcaccgag caggtcggac agggaccgaa cttcgcggtc gacccattca
     9481 accccgcgaa tctgccgcac tggagcggcg agacctaccc gagcgacttc gcaagcggcc
     9541 tcggcggcga cttcccgcgc aacgtgtggc agctttctcc gggcgagctc gagcgctggg
     9601 gcgatatcta ctcgaaccgc gatccggccg cgcgccgctt ctggtccggc gaattctcgc
     9661 tgcgcgagaa ggtgcaggcg atctacgcga tggccaatct cgacgggtcg aactggagcg
     9721 ccaacttcgg cgtgcgcgcc gtgcagacca aggagcgcgt gctcgtcaac gtcgccattc
     9781 cgacgagcat ctgcgccgtg caggcgccgt gtccgcaggt gccgggcgcg atcaccacgt
     9841 cggcgttcgg ctcgttctac cagtcgccga tcgagcacac gtacaccgac gtgctgccga
     9901 gcgccaacat caagttcgac ctgacgcgca acctcgtcgc gcgctttgcg gcggcgcgca
     9961 cgatggcgcg gcccgacttc agcgcgctcg gcgggtcgat cagcgccgac gacacgacac
    10021 ataccggcaa cggcgggaac cccgacctga agccgatccg ctcgacgaac ctcgacgcgg
    10081 cgctcgagtg gtatttccag ccgcgctcgc tgctgtcggc cgacgtgttc tacatggacc
    10141 tgtcg
//
LOCUS       DASSVX010000064         8334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_968961, whole genome shotgun sequence.
ACCESSION   DASSVX010000064 DASSVX010000000
VERSION     DASSVX010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968961"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFO53_02965"
     CDS             complement(<1..240)
                     /locus_tag="VFO53_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HET8875265.1"
                     /translation="MPNPPFDRLRVVLVRTSLPANIGAAARAMLTMGLARLVLVAPRS
                     FPHADATALAAGATSVLDAARVVATLDDALAGASFS"
     gene            303..1145
                     /locus_tag="VFO53_02970"
     CDS             303..1145
                     /locus_tag="VFO53_02970"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET8875266.1"
                     /translation="MHPMLTTAVKAARRAGTIITRGARDLDLLTVTAKGPKDFVSEVD
                     RAAEAAIVETLHAAYPDHGIFAEEGTGRGRNPDAEHVWIIDPLDGTTNFLHGFPQYCV
                     SIALAHKGVVTQGVIYDPVRNDLFTATRGRGAFLNDRRMRVSRRTHLRDCLIGTGFPF
                     RDGSHLDTYLAMMKTMVVQSAGLRRPGAAALDLAYVAAGFYDGFFEIGLNAWDVAAGS
                     LLVLEAGGLIGDLAGEGGYLHSGQVIAATPKVFAQMVQVLAPHRGDLARDLAAAEQPR
                     NQAR"
     gene            complement(1183..2286)
                     /locus_tag="VFO53_02975"
     CDS             complement(1183..2286)
                     /locus_tag="VFO53_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET8875267.1"
                     /translation="MLRPASRFDQVLTLAVLAMLIAGCYLVLQPFLTALVWAAILCTT
                     TWPLYLRLHARFGRRDALAALAMVLLLSLLMLAPFIVVGATIADNAERMATWGRQILA
                     AGPPEAPAWVAGLPLIGRTIADYWNSMAHDTAQLLAFFAQYVEPVRRFAVASGASVLG
                     AVLQLALSIFIAWFMFRDGHKIGERLRVAGERIAGERGTHLATVATATVRGVVLGILG
                     TALVQGVVAAIGFWIAGIRAAPLLGLLTFVVSPVPVGPPLVWVPAGLWLINDGQTGWG
                     IFVLLWGVLAVSTIDNVIKPLIISRGSVLPFMLVLLGVLGGAIAFGFIGVFLGPVLLA
                     VGYALLLEWAALRTGIEIVATDTDARKSTRGGP"
     gene            complement(2317..4941)
                     /locus_tag="VFO53_02980"
     CDS             complement(2317..4941)
                     /locus_tag="VFO53_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET8875268.1"
                     /translation="MKRRALALADALPPAACAAPMAPAEGIRLDHDRTLSQIAHGLNW
                     IGGAFLIASAAVIVWAVFRTVGVDDLLVALTVAGLGLGMPAIVAFVVAWILNSLGHAG
                     AESDDEVAAREVAPQRPNLTSLAWAYAVAVLACIAAWGVRVLLDPLLGQQVTYAPLLL
                     SVAFTAWYGGLGPAAFATLLGAGISWYAYLSPRDHFGALDIDDAVQLGLYCAAALCIG
                     GIASALRASRERAQTLAREVLSREAGLERTRGELAAERDRMQITLQSIGDAVIATDAQ
                     GNITFSNDCAAHLTGWPAAEAIGKPLVKVFRTLDEHTRRTTTVSLGDAQQDATAGMML
                     VARDGTERAIESKVSAIRGRSGSPGGFVLVFRDTTQARRARAALEESEARFRVMADQT
                     PVPLWMSDPDRRYVYVNRAWLVFTGRAADDELGDGWTAGIHPEDFGPRQSAFAEAFDA
                     RRSFTLEYRLRRDDGEYRWVLDHGASRFDGDGTFAGYIGACLDITERKEAASRLGIFE
                     QRKTAFLTSLAHELRNPLAPIRSSVELLQRMPAAGDPRVVRAQQIIERNCARLAGLVD
                     DLLDLSRIDSGNAPLARESVDVAAVIERAAARHAAPMRDRGQTLALELPRERLRVDGD
                     AARIEQMLAILIGNASSHSSPEGQIAVAARASGDAVAISVCDSGTGIPDEMQPLLFDL
                     FDKASEATANAPQGLGTGLAIVARLARLHGGSVSVDSGGPGKGSRFVVSLPAARPEAA
                     TNPATEPSEGAQRAVRLLVVDDNVDAADAIATLLSLNAYEVSTAHDPDEAMELGAAFD
                     PDVILLDIGLPGMTGYELARKLHADRVCRRAKLIAITGYGQPGDTDQAREAGFDGYLV
                     KPVDLEQLHARIDAILGG"
     gene            complement(5044..5238)
                     /locus_tag="VFO53_02985"
     CDS             complement(5044..5238)
                     /locus_tag="VFO53_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875269.1"
                     /translation="MPRKARDPRAPTLIDAHVHGSGRERAADVLRGLADAAKGAPRAS
                     SPGPPRMPLAGRTAGAAPDR"
     gene            complement(5219..7822)
                     /locus_tag="VFO53_02990"
     CDS             complement(5219..7822)
                     /locus_tag="VFO53_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET8875270.1"
                     /translation="MNELVVFSHLRWGFVHQRPQQILSRFARTWRVLFVEEPVYSAGD
                     PHAELAAPAPNVTVLTPHTPLTAPGFHDDQIPLLQKLVGQALARERFGDYGAWLYTPM
                     ALPLLTKLAPRIVVYDCMDDLAAFKAAPRQLVQRENALLRVANVVFAGGPSLYETRKS
                     RHHNVHLFASSVDSDHFARGGDVALTHPEVKALARPRLGYFGVLDERLDIALLRAVAE
                     ARPEWEICMVGPTQKIDPAALPRLPNLHYFGQRRYGELPAFLAGWDIAILPFAHNDAT
                     RFISPTKTLEYMAAGRAVVSTSIGDVKRLYGSVVRFGDTPERFIAECEAALVEPAVLR
                     SHRFAAMRRIVGATSWDETVREMRRVIDVCAQRGLTEAARMMLEPAKQPMAPITAHAP
                     LTGVPCLILGAGPTGLSAAYHYGAGSVVLEREAKVGGWCRSIEDTGFTFDHAGHVMFS
                     DDPYVQDLYRLLLGDNVHWQEREAWVHSKGVYTRYPFQGALHGLPPEVLRECLVGAIE
                     ARFGAPSRSGDGIDGSADVKDCCADATGIESHAALDASEAPPRNFEEFIHRTWGAGVA
                     KHFAIPYNRKLWTIPLCEMDTSWLSGRVPLPDLEEMIEGALQPVPKPMGPNARFGYPL
                     RGGFQALMNGFLPLLRAELVLQANVERVSPLLRAVTLADGRRFHYDTLVSTLPLPVLI
                     AAMGEEAPAEIRRAASELRHVSIRCVNLGVARPHLSDRHWIYFPEDTVFHRVFLQGNA
                     SPHCNPPGGFGLTCEISYSPTKPLPAVGAALTERCVQDCIRVGLLDASDALLTSNQLD
                     MPYAYVVYDHGRAESVAKIRTWLANFDIILSGRYSEWEYYNSDHAFIAGKKAAETAQR
                     LATTRLAAKSA"
     gene            complement(7973..>8334)
                     /locus_tag="VFO53_02995"
     CDS             complement(7973..>8334)
                     /locus_tag="VFO53_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875271.1"
                     /translation="DPGANGTAERFVSVLIGVSSSHVQQGQHRVSSKRARLSPYVPIC
                     VVETARSRHRGARQRTPDGAHYRGHCNAKQQLPDASVILTEKQPIGALHAMPRADGGN
                     DVECARGMDFAESPNSN"
BASE COUNT         1352 a         2891 c         2840 g         1251 t
ORIGIN      
        1 cgagaacgaa gcgccggcga gcgcgtcgtc gagcgtggcg accacgcgcg ccgcgtcgag
       61 caccgacgtt gcgcccgcag cgagcgctgt cgcatcggcg tgcggaaagc tgcgcggcgc
      121 caccagcacg aggcgcgcga gccccatcgt gagcatcgcg cgtgccgcag cgccgatgtt
      181 cgccggcagg ctcgtgcgca cgagcacgac gcgcaaccgg tcgaagggtg gattcggcac
      241 ggttgcgtag aatacgcgac ctggcgggcc gtcacagccc gccggcgcga acaggtccca
      301 ccatgcatcc gatgctcacc acggcggtca aggcggcacg ccgcgccggc accatcatca
      361 cgcgcggcgc gcgtgacctc gatctgttga ccgttaccgc caagggcccg aaggacttcg
      421 tcagcgaagt cgaccgcgca gccgaggcgg cgatcgtcga gacgctgcat gccgcgtacc
      481 ccgatcatgg catcttcgcc gaggaaggca ccggccgcgg ccgcaacccc gacgccgagc
      541 acgtgtggat catcgacccg ctggacggga cgaccaactt cctgcacggc tttccgcagt
      601 attgcgtgtc gatcgcgctc gcgcacaagg gcgtggtcac gcagggcgtc atctacgacc
      661 cggtccgcaa cgacctcttc accgcgacac gcgggcgcgg cgcgtttctc aacgatcggc
      721 gcatgcgcgt gtcgcggcgc acgcacctgc gcgattgcct gatcggaacc ggctttccct
      781 ttcgcgacgg cagccacctc gacacctatc tcgcgatgat gaagacgatg gtggtgcaaa
      841 gcgccggctt gcgccgcccg ggtgcagcag cgctcgatct cgcgtacgtc gccgccggct
      901 tctacgacgg cttcttcgag atcggcctca acgcgtggga cgtggcggcg ggaagcctcc
      961 tggtcctcga agcgggcggc ctcatcggcg atctcgcggg cgaaggcggc tatctgcaca
     1021 gcggacaggt gattgccgcg acgccgaagg ttttcgcgca gatggtgcag gtgctcgcgc
     1081 cgcatcgcgg cgatctcgcc cgcgatctcg cggctgccga gcaaccgagg aatcaggccc
     1141 gctgaatcgc gtcgcctcgg tgtgcttgac ggcgcgccgg tatcagggac cgccgcgcgt
     1201 tgatttgcgg gcgtcggtat ctgtggcgac gatttcgatg ccggtacgca gcgccgccca
     1261 ctcgagcagc aacgcatagc cgaccgcgag caggacgggg ccgagaaaca cgccgatgaa
     1321 cccgaacgcg atggcaccgc caagcacgcc gagcagcacc agcatgaacg gcagcacgct
     1381 gccgcggctg atgatgagcg gcttgatgac gttgtcgatc gtcgacacgg cgagcacgcc
     1441 ccacagcagc acgaagatgc cccatccggt ctggccgtcg ttgatgagcc acaggccggc
     1501 gggtacccac acgagcggcg gaccgaccgg caccggcgat acgacgaacg tgagcaggcc
     1561 gagcagcggc gccgcgcgaa tgccggcgat ccagaagccg atcgccgcaa ccacgccctg
     1621 cacgagcgcg gtgcccagga tgccgagcac gacgccgcgc acggttgccg tggccacggt
     1681 ggcaagatgc gtcccgcgct cgccggcaat ccgctcgccc gccacacgca gccgctcgcc
     1741 gattttgtgc ccgtcgcgga acatgaacca cgcgatgaag atcgacagcg cgagctgcag
     1801 cacggcgccg agcaccgatg cacccgacgc aaccgcgaac cggcgcaccg gctcgacgta
     1861 ctgcgcgaag aacgcgagga gctgcgcggt gtcgtgcgcc atgctgttcc agtagtcggc
     1921 gatcgtgcgc ccgatcaatg gcagcccggc gacccaggca ggcgcttccg gtggtcctgc
     1981 ggcgaggatc tgccggcccc acgtcgccat gcgctcggcg ttgtcggcga tcgtcgcacc
     2041 gacgacgatg aacggcgcca gcatcaggag cgacaacagc agcaccatcg cgagtgcagc
     2101 gagcgcatcg cgccttccga accgcgcgtg cagccgcaga tacagcggcc acgtcgtcgt
     2161 gcagaggatc gcggcccata cgagcgcggt gaggaacggc tgcagcacga gatagcaccc
     2221 ggcgatcagc atcgccagga cggcaagcgt caggacctgg tcgaaccgcg acgccgggcg
     2281 cagcatcgag cgctggtgga ctagggccgg ttcgccctag cctccgagga tcgcatcgat
     2341 ccgcgcatgc aactgctcga gatcgacggg cttgacgagg tagccgtcga agccggcctc
     2401 gcgcgcctgg tcggtgtcgc ccggctggcc gtacccggtg atcgcaatca gcttcgcgcg
     2461 ccggcagacg cggtccgcgt ggagcttccg tgcaagctcg tatccggtca tgccgggaag
     2521 cccgatgtcg aggaggatga catcgggatc gaacgcggcg ccgagctcca tcgcctcgtc
     2581 ggggtcgtgc gctgtcgaca cctcgtaggc attgagcgac agcaacgtgg cgatcgcgtc
     2641 ggcggcatcg acgttgtcgt cgacgaccag cagtcgcacc gcgcgctgcg cgccttccga
     2701 aggctccgtg gcggggttcg tcgccgcttc cgggcgggcg gccggaagcg acaccacgaa
     2761 gcggcttccc ttgccgggac ccccgctgtc cacgcttacc gacccgccgt gcagacgcgc
     2821 aaggcgcgcg acgattgcaa gccccgtgcc gagcccctgc ggcgcgttcg cggtggcctc
     2881 gctcgccttg tcgaacaggt cgaacagcaa cggttgcatc tcgtcgggaa tgccggtgcc
     2941 gctgtcgcat accgagatag cgaccgcgtc accgctggcg cgcgccgcaa cggcgatttg
     3001 cccttccggc gacgagtggc tcgacgcatt gccgatcagg atcgcaagca tctgctcgat
     3061 gcgtgcggca tcgccatcca cgcgcagacg ctcgcgcggc aactcgagcg cgagcgtctg
     3121 tccccgatcg cgcatcggcg ccgcgtggcg tgccgcggcg cgctcgatga cagcggcaac
     3181 gtcgacgctt tcgcgcgcaa gcggcgcatt gccgctatcg atgcgcgaca ggtcgagcag
     3241 gtcgtcgacg aggcctgcga ggcgcgcaca gttgcgctcg atgatctgct gtgcgcgcac
     3301 gacgcgcggg tcgcctgccg caggcatccg ctgcagcagc tccacggagc tcctgatcgg
     3361 cgccagcggg ttgcgcagct catgcgcgag cgacgtcagg aacgccgtct tccgctgctc
     3421 gaaaatgccg agcctcgagg cggcttcctt gcgctcggta atgtcgaggc atgcgccgat
     3481 atagccggcg aacgtgccgt ctccgtcgaa gcgcgacgcg ccatggtcga gcacccaccg
     3541 gtactcgccg tcgtcgcgac gcaggcggta ttcgagcgtg aacgagcgcc gcgcgtcgaa
     3601 ggcctcggcg aacgccgatt gccgcggacc gaaatcctcg ggatggatgc ctgcggtcca
     3661 gccgtcgcca agctcgtcgt cggccgcgcg gccggtgaac acgagccacg cgcggttcac
     3721 gtagacgtaa cggcggtcgg gatcggacat ccacaggggc accggcgtct gatcggccat
     3781 cacgcgaaag cgcgcctcgc tctcctcgag tgccgcccgt gcgcgccgcg cctgcgtcgt
     3841 gtcgcggaac acgagcacga agccgccggg agaaccgctg cggccgcgga tcgcgctcac
     3901 cttcgactcg atggcgcgct cggtgccgtc gcgcgcgacg agcatcatcc ccgcggtggc
     3961 atcctgctgc gcatcgccga gcgacaccgt cgtcgtgcgg cgcgtgtgct cgtcgagcgt
     4021 gcggaacacc ttcacgagcg gcttgccgat cgcttcggcc gccggccatc cggtgagatg
     4081 cgccgcgcaa tcgttcgaga acgtgatgtt cccctgtgca tcggtggcga ttaccgcgtc
     4141 gccgatcgac tgcagcgtga tctgcatgcg gtcacgctcc gcggcgagct cgccgcgtgt
     4201 gcgctcgaga ccggcttcgc gcgacagcac ctcgcgtgcg agggtctgcg cgcgctcacg
     4261 cgaagcgcgc aacgccgacg cgatcccccc gatgcaaagc gcggcggcgc agtagagccc
     4321 gagctggacg gcatcgtcga tgtcgagcgc gccgaagtgg tcgcgcggac tcaggtacgc
     4381 gtaccacgat atgccggcac cgagcagcgt tgcgaatgcc gccggaccga ggccgccgta
     4441 ccaggcggtg aacgcgaccg acagcagcag tggcgcgtag gtgacctgct ggccgagcag
     4501 cggatcgagc aggacgcgca cgccccacgc cgcgatgcat gcgagcaccg ccaccgcgta
     4561 cgcccaggcg agactcgtga ggttcggccg ttgcggcgcc acttcgcgcg ccgccacctc
     4621 atcgtcgctt tccgcaccgg catggccgag cgaattcagg atccaggcga cgacgaacgc
     4681 gacgattgcc ggcatgccga gcccgagtcc cgcgacggtc aatgcgacca gcaggtcatc
     4741 gacgccgacc gtgcggaaga ccgcccagac gatgaccgca gcgctcgcaa tcaggaacgc
     4801 gccgccgatc cagttgaggc catgcgcgat ctgggaaaga gtgcggtcgt ggtccaaacg
     4861 tatcccttcg gccggtgcca tcggtgccgc gcaggcagcg ggcggcaacg cgtcggccaa
     4921 cgcgagtgca cggcgcttca tcgcttcgtt ttccccgtca tggtcccggc cgtcgattgc
     4981 gccgtggctc cattgtccgt catcgtcccg gccgtcgatt gcgccgcgga gccattgtat
     5041 cggtcagcga tcgggggccg cgccggctgt gcgaccggcg agcggcatgc gcgggggtcc
     5101 cggcgacgac gcgcgcggcg cgcccttcgc cgcatcggcg agcccgcgca gcacgtcggc
     5161 tgcgcgctcg cgtccgctgc cgtggacgtg cgcgtcgatc agcgtcggcg cgcgtgggtc
     5221 acgcgctttt cgcggcaagc cgtgtcgtcg ccagccgctg cgccgtttcg gcggccttct
     5281 tgccggcgat gaatgcgtga tcggagttgt aatattccca ctcgctgtag cggcccgaca
     5341 gaatgatgtc gaagttcgcg agccacgtgc ggattttcgc gacgctttcg gcgcggccat
     5401 ggtcgtacac cacgtacgca tacggcatgt cgagctggtt cgacgtgagc agcgcatcgg
     5461 acgcatcgag gagcccgaca cggatgcagt cctgcacgca gcgctcggtc agtgccgcgc
     5521 cgacggcggg caagggcttc gtcggcgagt agctgatctc gcaggtgagt ccgaacccgc
     5581 cgggtggatt gcaatgcggg cttgcgttgc cctgcaggaa cacgcggtgg aagacggtat
     5641 cttcgggaaa atagatccag tgccggtcgg acaggtgcgg ccgtgcgacg ccgaggttga
     5701 cgcagcggat cgacacgtgg cgcaactcgc tcgccgcgcg gcggatctcg gcgggcgcct
     5761 cctcgcccat cgcggcgatc aggacgggca gcggcagcgt gctgacgagc gtgtcgtagt
     5821 ggaaccgccg cccgtcggcg agcgttaccg cgcgaagcag cggcgacacg cgctcgacgt
     5881 ttgcctgcaa tacgagctcg gcgcgcagaa gcggcagaaa gccgttcatc agcgcctgga
     5941 atccaccgcg cagcgggtag ccgaagcgcg cattcggtcc catcggcttc ggcaccggct
     6001 gcagcgcgcc ctcgatcatc tcctcgaggt cgggcagcgg gacgcgcccg ctgagccacg
     6061 acgtgtccat ctcgcaaagc ggaatggtcc acagcttgcg gttgtacgga atggcgaagt
     6121 gcttcgcgac gccggcgccc catgtgcgat ggatgaattc ctcgaagttg cgcggtggcg
     6181 cctcgctcgc gtcgagcgct gcgtgcgact cgatgccggt tgcatccgcg cagcaatcct
     6241 tcacgtcggc cgatccatcg atcccgtcgc cgcttcgtga cggcgcgccg aagcgggcct
     6301 cgatcgcgcc caccaggcat tcgcgaagca cttccggcgg cagcccgtgc agcgcgccct
     6361 ggaacgggta gcgcgtgtag acgcccttgc tgtggaccca ggcttcgcgc tcctgccagt
     6421 gcacgttgtc gccgagaagc agccgataga gatcctgcac gtacgggtcg tccgagaaca
     6481 tgacgtgccc cgcgtgatcg aacgtgaaac ccgtgtcctc gatcgagcgg caccacccgc
     6541 ccaccttcgc ctcgcgctcg agcacgacgc tgcccgcacc gtagtgatac gcggccgaca
     6601 gcccggtcgg tccggcgccg aggatgaggc atggcacccc ggtcagcggc gcgtgcgcgg
     6661 ttatcggcgc catcggctgc ttcgcgggct cgagcatcat gcgcgcggct tcggtaaggc
     6721 cgcgctgcgc gcagacgtcg atgactcgac gcatctcgcg caccgtttcg tcccaggacg
     6781 tcgccccgac gatgcggcgc atcgccgcga atcggtggct ccgcagcact gctggctcga
     6841 caagcgccgc ttcgcattcg gcgatgaacc gctccggcgt atcgccgaag cgcaccacgc
     6901 tgccgtagag ccgcttcaca tcgccgatcg acgtcgacac gaccgcgcgc ccggcggcca
     6961 tgtattcgag cgtcttcgtc gggctgatga agcgcgtcgc gtcgttgtgc gcgaacggca
     7021 ggatcgcgat gtcccagccc gcgaggaacg ccggcagctc gccgtacctg cgctggccga
     7081 agtagtggag attgggcagc cgcggcagcg ccgcgggatc gatcttctgc gtcgggccca
     7141 ccatgcagat ttcccattcg ggccgcgcct cggcaaccgc gcgcagcagc gcaatgtcga
     7201 gccgctcatc gagcacgccg aagtagccga ggcgcggccg cgcaagcgcc ttcacttcag
     7261 gatgggtcag cgcgacgtcg ccgccgcgcg cgaaatgatc gctgtcgacg ctgctcgcga
     7321 agaggtgcac gttgtggtgg cgcgactttc tcgtctcgta caggctcggg ccgcccgcga
     7381 atacgacgtt cgcgacgcgc agcaatgcgt tctcgcgctg caccagctgc cgcggcgccg
     7441 ccttgaatgc ggccagatca tccatgcagt cgtagacgac gatgcgcggc gcgagcttcg
     7501 tcagcagcgg cagcgccatc ggcgtataga gccatgcgcc gtaatcgcca aaccgctcgc
     7561 gcgccagcgc ctgcccgacg agcttctgca gcagcgggat ctggtcgtcg tgaaagccgg
     7621 gagcggtcaa cggcgtgtgc ggcgtgagca cggtcacgtt cggcgcgggg gccgcgagct
     7681 ccgcgtgcgg atcgccggcc gagtacacgg gctcctcgac gaagagcacg cgccaggtgc
     7741 gcgcaaaccg cgacaggatc tgctgcggac gctgatggac gaagccccag cgcagatgcg
     7801 agaacacgac gagttcgttc atgatgggtg cctgtgatgc gaagcgtcgg cgagcgaatc
     7861 gatgcgcgcc ctcacgtcgc gcgaggggtc cacgccacct gcgtgccggc agcccgggca
     7921 cattgcgcgt gcagcgtcgc cggcgcaaaa tcgcgcatcg atgcgtgcat ggtcaattcg
     7981 agttcgggct ttctgcaaag tccatgcccc gtgcgcattc gacgtcgttg ccgccgtctg
     8041 cacgcggcat tgcgtgcaat gcgccaatcg gctgtttctc cgtaagaatt accgaagcgt
     8101 ccggcaattg ctgctttgca ttgcaatgcc cgcggtaatg cgcgccgtcc ggcgttcgct
     8161 gtcgagcgcc gcggtgtctc gaacgcgccg tttcgacgac gcatatcggc acgtacggcg
     8221 acaagcgggc acgcttgctg ctcacgcggt gttgcccttg ctgcacatgc gaactggaca
     8281 ctccgatgag aactgaaacg aaccgctccg ccgtcccgtt cgcacccgga tcgc
//
LOCUS       DASSVX010000065         5536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1552431, whole genome shotgun sequence.
ACCESSION   DASSVX010000065 DASSVX010000000
VERSION     DASSVX010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552431"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1638)
                     /gene="ubiB"
                     /locus_tag="VFO53_03000"
     CDS             complement(106..1638)
                     /gene="ubiB"
                     /locus_tag="VFO53_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HET8875272.1"
                     /translation="MRLGRLLRIILVALRHGLDELALGHPRLRPLRLGVRYTLFWRNL
                     STPRGVRLRRALEDLGPIFIKFGQMLSTRRDLLPPDIADELALLQDRVPPFASELVVA
                     TIERVFGRPLDQVFRSFDLTPVASASVAQVHFAELPNATPVAVKVLRPNIGDVIEHDL
                     ALMRTAAALVDRLWREGRRLRPREVVAEFDKTIHDELDLMREAANCAQLRRNFRLSPL
                     LRVPEVYWDWTASEILVMERMAGIPIGRIADLVTAGVDLKRLARAGVEIFFTQVFRDG
                     FFHADMHPGNIFVGIDAANHGKYVALDFGIMGTLSERDKSYLAKNFLAFFRRDYHRVA
                     MTHVESGWVPANTRIDELTSEIRVVCEPIFDRPLKEISLGKVLVQLFRASRRFNVEIQ
                     PQLVLLQKTLLNVEGLGRQLDPDLDLWTTAKPFLERWMEERIGFRALQRRIFAEAASL
                     VETLPELPRLLHERLSAPPAASDVALRALALAQRSRNRWLGVIALLLTGVIVLLLLGS
                     EL"
     gene            complement(1808..2395)
                     /locus_tag="VFO53_03005"
     CDS             complement(1808..2395)
                     /locus_tag="VFO53_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875273.1"
                     /translation="MALTAAAANRLLEREAWARERLAAFAGRAFMVRVGPVTSGFRID
                     SRGLLESAPLAGSTPDLELTLSPFNVPAMLADPRRWNEFVAEEGDAELASVLKDLAQA
                     LPWFVERGLGRSLGPILGQRVADAGRAALTLPEYAATRIAANVGSYARDEAQVLAHPA
                     DVRALADDTALLAARIDALDARVRMLAERLPAPNP"
     gene            complement(2395..3318)
                     /locus_tag="VFO53_03010"
     CDS             complement(2395..3318)
                     /locus_tag="VFO53_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HET8875274.1"
                     /translation="MKRLFVIALAAAGSFALVHADFADAARMGGGRSFGMQRSIVPPA
                     ARTTPSTGNAFSGPAERPVMPATPGATAARPAAGAAAAGTASRTSRWLGPIAGLAAGL
                     GLAALFSHLGLSEGLGSLLLIGLLVVGAIALLRAFAARRASPPMQYAGTRSAAPPNAF
                     EPARPPAIDASWGAPAGATAAGVTYPPGFDPLPFIAQAKQQFRRLQAAYDRGDRALLG
                     DVTTPQMQAELSRDLADRAPQIPTEIVTLDADVAEVVQEGDRYIASVRFHGTLREDGG
                     TTAQPFEELWHLVKPVDGSTGWLLAGIQQYA"
     gene            complement(3460..4191)
                     /gene="ubiE"
                     /locus_tag="VFO53_03015"
     CDS             complement(3460..4191)
                     /gene="ubiE"
                     /locus_tag="VFO53_03015"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HET8875275.1"
                     /translation="METSKFGYEDVTPDEKTRRVRGVFDSVARKYDVMNDLMSAGMHR
                     GWKRFTVEASGVRPGARVLDLAGGTGDLARLFAARVGPEGSVVHTDINGAMLAAGRDR
                     LLDAGIVLSTVQCNAEALPFRDRSFDCVSIGFGLRNVTHKDRALAEMARVLAPGGLAA
                     VLEFSRIRAPLGRAYDWYSFTVLPLLGRLVAGDAASYRYLAESIRVHPDQEALKSMME
                     RSGFDRVDYYNLAAGAVALHVGRVF"
     gene            complement(4202..4579)
                     /locus_tag="VFO53_03020"
     CDS             complement(4202..4579)
                     /locus_tag="VFO53_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HET8875276.1"
                     /translation="MAGLDSTTPQPTEIKLHQASRVLEIAFANGRHFRLPYEFLRVHS
                     PSAEVRGHGPGQETLQTGKRDVTIDSVEPVGHYALRPTFSDGHGTGIYSWDYLYDLGE
                     RQDELWRRYLERLAAAGASRDAA"
     gene            complement(4594..>5536)
                     /locus_tag="VFO53_03025"
     CDS             complement(4594..>5536)
                     /locus_tag="VFO53_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET8875277.1"
                     /translation="ESTKECNFAISPPGVGRFRVNAFIQQGRVGMVLRTITTHIPKFD
                     DLGLPSVLKDIIMSKRGIMMFVGATGAGKSTSMASLLGYRNENSFGHIITIEDPIEFV
                     HEHRNCIVTQREVGVDTETWDIALRNSMRQAPDVILIGEVREKSTMDYAIAFAETGHL
                     LLATLHANSTNQALDRIINFFPEERRAQLLMDLSLNVRAFVAQRLIPRRDGKGRVPAV
                     EIMLNSPLVSDLIFKGDVGGIKDLMKKSRELGMQTFDQSLFDLYEAGLITYEDALRNA
                     DSLNDLRLDIKLKGKEAKNRNMFSGIGNLDIVPETNQ"
BASE COUNT          950 a         1832 c         1916 g          838 t
ORIGIN      
        1 gtgcccgtcc ccgactgctg gcgccgcagg taattacagc tctgaccccg atagcgattg
       61 cggcgcgaag ggcgtgcccg tccccgactg ccggcgccgc aataattaca gctctgaccc
      121 caatagcagc agcacgatga cgccggtgag cagcaacgcg atcacgccga gccagcggtt
      181 gcgcgatcgc tgcgcgagcg ccagcgcccg caatgcgaca tccgacgctg cgggtggcgc
      241 cgacagccgc tcgtgcagca ggcgcggaag ctccggcagc gtctcgacga gcgacgccgc
      301 ttccgcgaaa atccgccgct gcagcgcgcg aaagccgatc cgctcctcca tccagcgctc
      361 gaggaacggc ttcgcggtgg tccacagatc gagatccggg tcgagctggc gcccgagccc
      421 ttcgacgttc agcagcgttt tctgcagcag cacgagctgc ggctggatct ccacgttgaa
      481 gcggcgcgac gcgcggaaga gctgcacgag caccttgccg agcgagatct ccttgagcgg
      541 ccggtcgaag atcggctcgc acacgacgcg gatctcgctc gtgagctcgt cgatgcgcgt
      601 attcgccggc acccagcccg attcgacgtg cgtcatcgcg acgcggtggt agtcgcgccg
      661 gaagaacgca agaaaattct tcgcgaggta gctcttgtcg cgctccgaaa gcgtgcccat
      721 gatgccgaag tcgagcgcga cgtacttgcc gtggttcgcg gcgtcgatgc cgacgaagat
      781 gtttcccgga tgcatgtcgg cgtggaagaa gccgtcgcga aacacctgcg tgaaaaagat
      841 ctcgacgccg gcgcgcgcca ggcgcttgag atcgacgccg gcggtgacga gatcggcgat
      901 gcggccgatc gggatgcccg ccatgcgttc catgacgagg atctcgctcg ccgtccagtc
      961 ccagtacacc tcgggcacgc gcagcagcgg cgagagccgg aagttgcgcc gcagctgcgc
     1021 acagttcgcc gcctcgcgca tcaggtcgag ctcgtcgtgg atcgtcttgt cgaactccgc
     1081 gaccacctcc cgcgggcgaa ggcgccggcc ttcgcgccaa aggcgatcga cgagtgccgc
     1141 ggccgtgcgc atcagcgcaa ggtcgtgctc gatcacgtcg ccgatattgg gccgcagcac
     1201 cttgaccgcg accggtgtcg cgttgggcaa ttcggcgaaa tgcacctgcg ccaccgacgc
     1261 gctcgcgacg ggtgtgaggt cgaacgagcg gaacacctgg tcgagcggcc ggccgaacac
     1321 gcgctcgatc gtcgccacga cgagttccga tgcaaacggc gggacccgat cctgcaaaag
     1381 tgcgagctcg tcggcgatat cgggcggcag cagatcgcgc cgcgtcgaca gcatctgccc
     1441 gaatttgatg aagatcggtc cgaggtcctc cagcgcacgc cgcagacgca cgccgcgcgg
     1501 cgtcgacaga ttgcgccaga agagcgtgta gcggacgccg agccgcagcg gacgaaggcg
     1561 cggatggccg agcgccagtt cgtcgagccc gtgccgtagt gcgacgagga tgattcgaag
     1621 gagccggccg agccgcatgc ggcgattgta cggtgccccc gctcgccgcc ccgcgacgcg
     1681 aaggcgagag ccgcaaacag ggtcagagac gactttcgca aaacacgcga aagagccgta
     1741 aacaggagcc gtaaacaggg tcagagacga ctttcgcaaa acacgcgaaa gtcgtctctg
     1801 accctgttta cgggttcggc gccgggagcc tttcggcgag catgcgcaca cgcgcgtcga
     1861 gtgcgtcgat tcgcgcggca aggagcgcgg tgtcgtcggc cagcgcgcgc acatcggccg
     1921 gatgcgcgag cacctgcgcc tcgtcgcggg cgtagctgcc gacgttggcg gcaatgcgcg
     1981 tcgccgcgta ttcaggcagc gtgagtgcgg cgcggcccgc gtcggcgacc cgctgtccga
     2041 ggatcgggcc gagcgagcgg ccgagaccgc gctcgacgaa ccacggcagc gcctgcgcaa
     2101 gatccttcag gacgctcgcg agctctgcat cgccttcctc ggcgacgaac tcgttccagc
     2161 gccgcggatc ggcgagcatc gccggcacgt tgaagggcga cagtgtcagc tcgaggtcgg
     2221 gcgtgctgcc tgcgagcggt gcggattcga ggagcccgcg cgaatcgatg cgaaagccgc
     2281 tcgtcaccgg accgacgcga accatgaacg cacggccggc gaacgcggca agacgctcgc
     2341 gcgcccacgc ttcgcgctcg agcaggcgat tcgcggcggc ggcggtcagt gccactaggc
     2401 gtattgctga atgcccgcga gcagccaacc cgtcgagccg tccaccggct tgacgaggtg
     2461 ccatagttcc tcgaacggtt gcgcagtcgt accgccatct tcgcgcagcg tgccgtgaaa
     2521 gcggacgctc gcgatgtacc ggtcgccctc ctgcaccact tcggcgacat cggcgtccag
     2581 cgtcacgatt tcggtgggaa tctgcggcgc gcgatcggcg agatcgcggc tgagttcggc
     2641 ctgcatctgc ggtgtcgtca cgtcgccgag cagcgcgcgg tcgccgcggt cgtacgccgc
     2701 ctgtagccgg cggaactgct gcttcgcctg cgcgatgaac ggcagcgggt cgaagcccgg
     2761 ggggtaggtg acgccggcgg cagttgcgcc ggcgggagcg ccccaggacg catcgattgc
     2821 cggcgggcgc gccggctcga acgcgttcgg cggtgcagcc gatcgcgtgc ccgcatactg
     2881 catcggtggc gaggcgcggc gggcggcgaa cgcgcgcagc aatgcaatgg cgccgaccac
     2941 gaggaggccg atcaaaagca ggctgcccag gccctcggac aacccgagat gcgaaaacag
     3001 cgcggcgagg cccaagccgg cggcgaggcc cgcgatcggt ccgagccagc gcgaggtacg
     3061 cgaagcggta ccggcggccg cggcaccggc cgccggacgc gccgccgtag cacccggcgt
     3121 cgcgggcatg accggacgct cggccgggcc ggaaaacgcg ttgccggtgg aaggcgtcgt
     3181 gcgcgcggcg ggcgggacga tgctgcgctg catgccgaag ctgcggccgc cgcccatccg
     3241 ggccgcatcg gcgaaatcgg cgtgcaccag ggcgaacgag ccggcagccg cgagggcgat
     3301 gacaaaaagg cgtttcacgt gattcctccg gaaagtggcc gcgacttgcg gcacacggca
     3361 atgcccgtca tatgcgggct ggtgccgtcg ctgtcaatgc gggaaacccg catgcgcgca
     3421 gaaacctcga gaccggaccc atgaacgcgg gggtggccat caaaacacgc ggccgacgtg
     3481 caacgcgacc gcgccggcag cgaggttgta gtaatcgacg cggtcgaatc ccgatcgttc
     3541 catcatcgac ttcaacgcct cctgatcggg atggacgcgg atcgactcgg cgagatagcg
     3601 atagcttgcg gcgtcgccgg cgaccagccg tccgagcagc ggcaggacgg tgaagctgta
     3661 ccagtcgtag gcgcgtccga gcggggcgcg gatgcgggaa aactcgagga cggccgcgag
     3721 gccgcccggc gccagcacgc gcgccatctc ggcgagcgcg cggtccttgt gggtcacgtt
     3781 gcgaaggccg aagccgatcg acacgcaatc gaacgaccgg tcgcgaaacg gaagcgcttc
     3841 cgcgttgcat tgcacggtgg acagaacgat gcccgcgtcg agcaggcgat cgcgtccggc
     3901 ggcgagcatc gcgccgttga tgtcggtgtg cacgacgctg ccttccggtc ccacgcgcgc
     3961 ggcgaacagc cgcgcgagat cgccggtgcc gcccgcgaga tcgaggacgc gcgcgccggg
     4021 tcgaaccccg gacgcttcga cggtgaagcg cttccagccg cgatgcatgc cggcggacat
     4081 gaggtcgttc atcacgtcgt acttgcgcgc gaccgaatcg aagacgccgc gcacgcggcg
     4141 cgtcttctcg tcgggcgtca cgtcctcgta gccgaacttg gacgtttcca tcgccgccgt
     4201 gctacgccgc gtcgcggctt gcgccggccg cggcaaggcg ttcgagatag cgccgccaca
     4261 gctcgtcctg acgctcaccg agatcgtaga ggtaatccca ggaataaatg ccggtgccgt
     4321 ggccgtcgga gaacgtcgga cgcagcgcgt agtggccgac gggctcgacc gagtcgatcg
     4381 tcacgtcgcg cttgccggtc tgcagcgttt cctgtccggg tccgtgaccg cgcacctcgg
     4441 ccgacggcga atgcacgcgc aggaattcgt acggcagccg gaagtggcgg ccgttcgcga
     4501 acgcgatttc cagcacgcgc gaggcctggt gcagcttgat ctcggtcggc tgcggggtgg
     4561 tcgaatcgag gccggccatg ccccgagatt acactactga ttcgtctccg gcacgatgtc
     4621 gaggttgccg atgcccgaga acatgttgcg gttcttcgct tccttgccct tgagcttgat
     4681 gtcgaggcgc aggtcgttca gcgaatcggc attgcgcagc gcgtcctcgt aggtaatgag
     4741 gcccgcttcg tagaggtcga acagcgactg gtcgaacgtc tgcatgccga gctcgcgcga
     4801 cttcttcatc agatccttga tgccgccgac gtcgcccttg aagatcaggt cggacacgag
     4861 cggcgaattc agcatgatct ccaccgccgg cacgcgcccc ttgccgtcgc gccgcggaat
     4921 caggcgctgc gcaacgaacg cgcgcacgtt gagcgacagg tccatcagca gctgcgcacg
     4981 ccgctcctcg gggaagaagt tgatgatccg gtcgagcgcc tggttcgtgc tgttcgcgtg
     5041 cagcgtcgcg agcagcaggt gtcccgtctc ggcgaacgcg atcgcgtagt ccatcgtgct
     5101 cttctcgcgc acttcgccga tcaggatcac gtcgggtgcc tggcgcatcg agttgcgcag
     5161 cgcgatgtcc caggtctcgg tgtcgacgcc cacttcgcgc tgcgtgacga tgcagttcct
     5221 gtgctcgtgg acgaactcga tcgggtcctc gatcgtgatg atgtgcccga agctgttctc
     5281 gttgcggtag ccgagcagcg acgccatcga cgtcgacttg ccggcgcccg tcgcgccgac
     5341 gaacatcatg atgccgcgct tcgacatgat gatgtccttc agcacggacg gcaggccgag
     5401 gtcgtcgaac ttcggaatgt gagtggtgat cgtgcgcagc accatgccga cgcggccctg
     5461 ctggatgaac gcgttcacgc ggaagcggcc cacgccgggc gggctgatcg cgaagttgca
     5521 ttccttcgtc gattcg
//
LOCUS       DASSVX010000066        15672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1269005, whole genome shotgun sequence.
ACCESSION   DASSVX010000066 DASSVX010000000
VERSION     DASSVX010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 15672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15672
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269005"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VFO53_03030"
     CDS             <1..949
                     /locus_tag="VFO53_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HET8875278.1"
                     /translation="VLARENARLMPTAMHPWMNPRRETWLWDGAIYRNYHRIYDCRGH
                     GWANLQSMHINLPFGDDGELARLHAAIRLVLPLLPALAASSPIADGAPTGLLDSRLDA
                     YRRICAITPHVAGSVIPETVETQAEYEAKILRPMYAQIAPHDPEGVLQHEWLNSRGAI
                     ARFDRRAIEIRVVDVQECPQADVAIAAATSAVIRSLYDARYAPLDEQQAIATEVLYGV
                     LLASIRDADGAVIDDVDYLHALGMPTSRCVARDVWRSLLADAGSAHAWWHPAIDVILQ
                     NGPLARRILRATGSRPDRDRLRVTYRALCQCLEDGRMFA"
     gene            961..1704
                     /locus_tag="VFO53_03035"
     CDS             961..1704
                     /locus_tag="VFO53_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HET8875279.1"
                     /translation="MSHRTRSVVLVTCEHGGNDIPHEYAPLFRGRRRLLDSHRGYDPG
                     ALDTAHDLARALGAALVVSTISRLLVELNRSPGRQFRNSPVMRASAKGTRDDICRRYY
                     LPYRRDVETFVAQAIAGRKRVVHVSSHSFTPLLDGHVRRADVGLLYDPERANERALCL
                     RWQRALARCRPQWIVRRNYPYRGVSDGFTSYLRKRFDDGAYAGLELEVNQKLVRSGAL
                     PALDRGAIARALCEALGREPRHLVSAVNA"
     gene            complement(1694..2584)
                     /locus_tag="VFO53_03040"
     CDS             complement(1694..2584)
                     /locus_tag="VFO53_03040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET8875280.1"
                     /translation="MKRMKVGDFPREVLTIFDGYVHGEIGRREFLDRAAKYAVGGMSA
                     AAMLESLRPDYALAQQVPKDDTRIHAEYLPYPSPRGSGTMRGYFVRPAKPSGKLPGIV
                     VIHENRGLNPYIEDVARRLAIENYVAFAPDALTPAGGYPGDDEKGARLFASLDATKRT
                     EDLLAAVPFLKSHPECNGSVGAVGFCFGGGIVNMMAVRFPDLAAAVPFYGAQPSAADT
                     AKIKAPLLIHYAGRDERIDAGWPAWKEALDRNHVAYREYTYPDVEHGFHNDTTPRYDE
                     AAAKLAWSRTLAFFGEHLKR"
     gene            complement(2738..3808)
                     /locus_tag="VFO53_03045"
     CDS             complement(2738..3808)
                     /locus_tag="VFO53_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875281.1"
                     /translation="MSNVTGGVVFDRITKRYNDVTAVDDVSFEVRPGQLVTLLGPSGC
                     GKTTTLRMVAGLEPATSGRIAIGGVDVTHRAANERDVSMVFQSYALFPHMNVLDNVCY
                     GPLSSGAGKAKARELAHAKLRMLGLAGLEQRLPSELSGGQQQRVAVARSLVLEPAVLL
                     FDEPLSNLDAKLRRRVRQEIRELQQTLDLTVIYVTHDQEEALAVSDQVIVMDRGRIAQ
                     QGTPSQLYEAPASRFLADFIGDANLVSGELVQGAEGASFRANDVSAPVRADGVPAGPA
                     TLAVRPHRLRLTAAGGGLLPAECRRAAYLGSRIEYVLATAWGELLVFDGGIHTPLARG
                     ARAGVAFAPEDAIVLPGTGSEL"
     gene            complement(3826..5985)
                     /locus_tag="VFO53_03050"
     CDS             complement(3826..5985)
                     /locus_tag="VFO53_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET8875282.1"
                     /translation="MATSLPSAALPAAHAPRAPRDLTTTLFAALALAALLLLPWAMDG
                     GASAIGIANGAGLAKWPRYAASAALVPVLLAVIATLACAWFGLTRFTALFAAAALAWT
                     FAQGFVAGVQGPPFGIGATIALAAITVCLARALGRIGLFAGNATIATIVVTIGLLLVL
                     FIFYPIGSALTSAVQDARGHFAPQLVAERLLTSDIWGLGCLGGGTRCGVAINSALLAT
                     IVGLLSTLLGLALALAVQRGGQRYAGVLKLMSVLPVITPPFVVALALVVLFGRTGLIT
                     SWLGVLGIPRSRWIYGLPGVAIAQLITFTPISFMLLHGALHAISPALEEAAQTLRGTP
                     GRVFRTVTWPLLRPALANAFLLAFVESLADFGNPIVLAGNFEVLSTKIFFAVAGAQYD
                     AGRAAVLAIVLLALTLLAFVLQQRWVGRASYVTVSGKGDGGAPAALPRRLWLTCFWIA
                     AAFIVFTLMCYAVILTGGFVRDIGRGDMTPSFRHFLDGFRLEWAARGLLFSGSAWDSL
                     FTTIEVALVAAPLTALVGLLSAYVITRHRFAGRRAFEFLTLVSFAVPGTVIGVSYIVA
                     FNIAPLELTGGMAILVLCFVFRNMPVGVRSGVASLAQIDRTLDEASATLRASTWRTLT
                     RVVMPLLRSAILVTLVFSFTRAMTAVSAVIFLATAKYNLSTVYIINRVEAGEYPLAIV
                     YSTVLMLFMLAVLLGMQRFVGEARLGRRAAAVVVAPQ"
     gene            complement(5985..7016)
                     /locus_tag="VFO53_03055"
     CDS             complement(5985..7016)
                     /locus_tag="VFO53_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8875283.1"
                     /translation="MRISAFLRALLPALLFAVPLAAAAQGELVVYCTVQEEWCRPMVA
                     AFEKKFGIKVLMTRKSAGEFYAQIKAESANPRGDIWWGGTGDPHLQAAEEGLTEPYKS
                     PMLAQLQDWAVRQAEESKYRTVGIYAGALGYTYNTELLKKKNVPEPMCWADLVKPQFK
                     DEVQVANPNSSGTSYNMLATLVQIMGEDKAFDYLKALHKNVNQYTKSGAAPARAAATG
                     ETMVGIVFQHDALYQILGGAPLKIVSPCEGTGYEIGSMSIIKGAKNMANAKKWYDWAL
                     TPEAQAIGLEAKVSYQLPSNKSAPVSPLAPKFSDIKLINYDFAKYGSSAERTRLLARW
                     DKEVSTLPK"
     gene            complement(7115..8419)
                     /locus_tag="VFO53_03060"
     CDS             complement(7115..8419)
                     /locus_tag="VFO53_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET8875284.1"
                     /translation="MARRTYAKGTLARFSRVAAIESCRRAVVALRARSIGYALACAAA
                     VLVSACGGGSSPSDTTQATDPALIEKGKQIFRFDTFGDETQWTDTLRMHEVISAAVDP
                     TTALTVGLKVDADALPAAVVAGIQDGSIDLKSPATTIALLKLDAVVGVKGTVETVNGH
                     DTLTRVGITCALCHSTVDNSFAPGIGKRLDGWPNRDLDPGAIIALSPAVPAASKAVYK
                     SWGPGKYDPRFNLDGLNGPQVIPPAYGLAGIHRITSTGDGDDIAYWNRYVGVTQMGGH
                     GTFSDRRTGVNVTNGTDDLISAKLPALQAYQLSLPAPPPPTGSFDPVAAARGKALFNG
                     AGTCATCHSGDALTDANLRLHPPTDVVSEPEPNGAPSYASRSATKQYRTAPLHGLWQH
                     APYFHNGSAATLEDVVRTYNQRKSLGLPDNQIADLVQYLKSL"
     gene            complement(8530..9534)
                     /locus_tag="VFO53_03065"
     CDS             complement(8530..9534)
                     /locus_tag="VFO53_03065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8875285.1"
                     /translation="MKLSVLDQSTAAEGASQHAAIQETLALARHCEALGYHRYWVSEH
                     HSSDSIVGTAPEVLMAAIAATTSRIRVGSAGVMLPHYSALKVAEQFRVLEAIAPGRID
                     LGVGRAPGSDRLTALALNPYSNAAEEFPRQVHELRAWVCGEPLDEGHPFAAISANPRG
                     PTCPELWILGSSDYGAQLAAHFGLPYAFAYFFTEGRGADQALALYRRNYRPSARHPAP
                     QATICVWALAADTEACARHLLKTREFWRVGFEKGLRKPLVTPEFADAYPYTDSERATI
                     DALRRKAIVGTGSAVAARLAQLAQHFALDEIVVVTWTHDPAARHRSYELLASAFHASE
                     "
     gene            complement(9531..10703)
                     /locus_tag="VFO53_03070"
     CDS             complement(9531..10703)
                     /locus_tag="VFO53_03070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HET8875286.1"
                     /translation="MRAACWHGKRDMRVDSVPDPVILNQRDAIVRVTSTAICGSDLHL
                     YNGFIPTMKKGDILGHEFMGEVVDVGRDVKNLRVGDRVVVPFPIACGNCGACEAQAYS
                     LCENSNPNAWMAEKLLGHSPAGIFGYSHMLGGFAGGQAEYARVPFADVGPLKVPAGIS
                     DEQALFLSDVLPTGYMAAKMCDIAPGDTIAVWGCGPVGQFAVASARLLGAARIIAIDR
                     VPERLALAANKAGATDTINYEDQDVLDTLKELTGGVGPDACIDAVGTEAHAPGIMYAY
                     DRFKQALMLESDRPIALRQAIVACRNGGTVSVIGVYGGFIDKFPMGSFMNRSLTMKTG
                     QCHVQRYMKPLLGRIESGEIDPSFIVTHRMHLDDAPDGYAMFANKQDDCIKVVLTP"
     gene            complement(10700..11707)
                     /locus_tag="VFO53_03075"
     CDS             complement(10700..11707)
                     /locus_tag="VFO53_03075"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET8875287.1"
                     /translation="MQGTDDWPPGPRDDAMPEVMRRPRHAAHASRDESRQLGEAGAER
                     LAHGLAWFSISLGLAEMLAPRLVSRLVGGNGKYTNLIRLYGLRELVSGLMIFGQGARP
                     AAAVWSRVAGDAVDIATLAAAGASSRMNRSGVAVAAAGVLGVTALDLYCARGLTRNRR
                     GEEAIRMTRSVAVNRPPHELYAFWREFENFPRFMYHVNSVRAIGPGLSHWTVGGPAGS
                     TIEWDARITADTPNELIAWQSLEGAGVESRGTVRFEPRPGNRGTIVRVDVLYRPPGGM
                     AGRLAAMLFNESPEQQIYDDLHRFKQIVETGEIVRSDGSPHGVGDVKQQPGQASEARP
                     R"
     gene            complement(11802..12545)
                     /locus_tag="VFO53_03080"
     CDS             complement(11802..12545)
                     /locus_tag="VFO53_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET8875288.1"
                     /translation="MLVATWNVNGIRAREAQFCDWLDRDRPDVVCLQELKAEPAQIPE
                     RCRHADYHVYWHGMRGYSGVSLHVNKALHGDAPAFAHPDFDMESRIVQVALGDLVVAS
                     VYVPNGGKDYQAKIAFLSKLADWAARLHAEGREVVLCGDLNVAHADIDVHPRERKAGV
                     IGQRPEERELFDRLLGGHFVDVGRALHPDDEAMFSWWPPWRNMRQRNIGWRIDYILAS
                     RSIADRVAECKVLADVGTSDHAPVIMSIA"
     gene            complement(12572..13231)
                     /locus_tag="VFO53_03085"
     CDS             complement(12572..13231)
                     /locus_tag="VFO53_03085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8875289.1"
                     /translation="MANRTLNLDDSLYAYVLAHSLREHPAQTALREATKAHPHAGMQI
                     GPEQGQFMALLVRLIGARRTLEIGVFTGYSALSVALALPDDGRVLACDVSDEYTRIGK
                     PYWEAAGVAHKIHLTLAPARATLDARLANGEANAFDFAFIDADKTAYDDYYERCLMLV
                     RPGGLIAIDNVLWGGSVAEPATTADTAALQALNDKLHRDERIDLSLLPIGDGLTLARR
                     R"
     gene            complement(13308..13856)
                     /locus_tag="VFO53_03090"
     CDS             complement(13308..13856)
                     /locus_tag="VFO53_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875290.1"
                     /translation="MSFDLGGLLQKYLNVPAGQAAPGAEEHFDQVAQHASPEVLGQGI
                     SDALRSDATPPLGQMVSDVYSKADPNQQAGMLNQILASLPQGALSSLGGGALSSLGGG
                     ALGGLLGQLTGGGTPQVTPQQASQISPSQVQDLANHAEQHGSGVFDALGNFYAQHPDL
                     VKRLGAAAITIAMANIAGRMRR"
     gene            complement(13871..14503)
                     /gene="msrA"
                     /locus_tag="VFO53_03095"
     CDS             complement(13871..14503)
                     /gene="msrA"
                     /locus_tag="VFO53_03095"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007353945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET8875291.1"
                     /translation="MRNLFFMMVFAALGAHAAVPDYAGPVSKASTPGEATAVFAGGCF
                     WGVDAVFKHVKGVTNVVSGYSGGSAATAKYMMVGTGATGHAESVKVTYDPSKVDYAEL
                     LKVFFSVAHDPTQLNRQGPDVGSQYRSAIFYANAQQKELAERYIAQLDAAKAFGKPIV
                     TQVAPLDKFYPAEDYHQNYLALHPEQPYIVYNDLPKLAELKKEFPAWYKP"
     gene            complement(14652..15293)
                     /gene="upp"
                     /locus_tag="VFO53_03100"
     CDS             complement(14652..15293)
                     /gene="upp"
                     /locus_tag="VFO53_03100"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET8875292.1"
                     /translation="MPEAAATVHVLDHPLIQHKLTLMRRRETSTTNFRRLLNEIGALM
                     AYEVTRDAPMNEVEIKTPLETMRSRLIDGKKLVFVPILRAGTGLLDGFLNVVPGARVG
                     HIGLYRDPKTLVAVEYYFKLPGGMHERDAIVLDPMLATGHSAVAAVERLKETRPRSIR
                     IVCLVACPEGIATFQEAHPDVPVYTAAIDRELDSHGYILPGLGDAGDRIFGTK"
     gene            complement(15293..>15672)
                     /locus_tag="VFO53_03105"
     CDS             complement(15293..>15672)
                     /locus_tag="VFO53_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1688 family protein"
                     /protein_id="HET8875293.1"
                     /translation="QWLAYSLFEPFEWAGIPVTDRDALTALPEYRNGGLLIDTGVLKL
                     DSCVDRGAQWAPESELVVEWRALTVTLLDELAQCVRAELDRPQLPLACILEGGTWAAG
                     RELAMRLRNGEPPLRVLSDGTVF"
BASE COUNT         2574 a         5293 c         5269 g         2536 t
ORIGIN      
        1 cgtgcttgcg cgcgagaatg cgcggctcat gcccaccgca atgcatccgt ggatgaaccc
       61 gcggcgcgaa acgtggctgt gggacggtgc catctatcgc aactatcacc gcatctacga
      121 ttgccgcgga cacggctggg cgaacctgca gagcatgcac atcaacctgc cattcggcga
      181 cgacggcgag ctcgcgcggc tccatgcggc gatcaggctc gtcctgccgc tccttcccgc
      241 cctcgccgcg agctcgccga tcgccgacgg cgcaccgacg ggattgctcg actcccggct
      301 cgatgcctac cgccggattt gcgcgatcac tccgcatgtc gccggcagcg tgattccgga
      361 aaccgtcgaa acgcaggccg aatacgaggc gaagatcctg cggccgatgt atgcgcaaat
      421 cgcaccgcac gatcccgaag gcgtgctgca gcacgagtgg ctcaattcgc gcggggccat
      481 tgcacggttc gatcgccgtg caatcgagat ccgggtcgtc gacgtgcagg aatgtccgca
      541 ggccgacgtt gccatcgcgg cggcgacgtc ggcggtcatc cggtcgctct atgacgcgcg
      601 ctacgcgccg ctcgacgagc agcaggcaat cgcgacggag gtgctgtacg gcgtcctgct
      661 cgcatcgatt cgtgatgccg atggtgccgt gatcgacgat gtcgactatc tgcatgcact
      721 cggcatgccg acctcgcgct gcgtcgcacg cgacgtctgg cgatcgctgc ttgccgatgc
      781 cggcagcgcg catgcctggt ggcacccggc aatcgacgtg atcctgcaaa acggcccgct
      841 cgcgcggcgg attctgcgcg cgaccggaag ccgtcccgat cgcgaccggc tgcgcgtgac
      901 ttaccgcgcg ctctgccagt gcctcgaaga cgggcggatg ttcgcatagc ggcaccgcga
      961 atgtcacacc gcacgcgcag cgtcgttctc gtcacgtgcg agcatggggg caacgacatt
     1021 ccgcacgagt acgcgccgct ctttcgcggc cgccggcgcc tgctcgattc gcatcgcggc
     1081 tacgatccgg gcgcgctcga cacggcgcac gatcttgcgc gcgcactcgg tgcggcactc
     1141 gtcgtctcga caataagccg gcttctcgtc gagctcaacc gctcgccggg gcggcagttt
     1201 cgcaactcgc cggtcatgcg cgcgagtgcg aagggcacgc gcgacgacat ctgccgacgt
     1261 tactacctgc cgtaccggcg cgatgtcgaa acgttcgtcg cacaggcgat cgccgggcga
     1321 aagcgtgtcg tgcacgtttc gtcgcacagc ttcacgccgt tgctcgacgg gcacgtccgg
     1381 cgcgccgacg tgggcctgct ctacgatccc gaacgggcga acgagcgcgc gctgtgcctg
     1441 cgctggcagc gcgcactggc ccgatgccgg ccgcagtgga tcgtgcggcg caattacccg
     1501 tatcgcggag taagcgacgg gttcacgtcg tatctgcgca agcgcttcga cgatggcgcg
     1561 tatgcaggcc tcgagctcga ggtgaaccag aagctcgttc gaagcggcgc cctgcccgcg
     1621 ctcgaccggg gagcgatcgc ccgtgcgctc tgcgaggcgc tcgggcgcga gccacggcac
     1681 ctcgtcagtg ccgtcaacgc ttgagatgct cgccgaaaaa ggccagcgtg cgcgaccatg
     1741 ccagcttcgc ggccgcctcg tcgtagcgcg gcgtcgtatc gttgtggaaa ccgtgctcga
     1801 cgtccgggta cgtgtactcc cgatacgcga cgtggttgcg gtcgagcgcc tccttccacg
     1861 ccggccatcc ggcgtcgatg cgctcatcgc gcccggcgta gtgaatgagc agcggcgcct
     1921 tgatcttcgc ggtatcggcg gcactcggct gcgcgccgta gaacggcacc gcggcggcga
     1981 gatcgggaaa gcgcacggcc atcatgttga cgatgccgcc gccgaagcag aaaccgacgg
     2041 caccgacgct gccgttgcat tcgggatgcg acttcaggaa cggaacggcg gcgaggaggt
     2101 cttcggtgcg cttcgtagcg tcgaggctcg cgaagagcct ggctcccttt tcgtcgtcgc
     2161 ccggataacc gcccgccggc gtcagcgcat cgggcgcaaa ggccacgtag ttttcgatcg
     2221 cgagccggcg cgcgacatcc tcgatgtacg ggttgaggcc gcggttctca tggatgacca
     2281 cgatccccgg cagctttccc gaaggcttcg cgggacgcac gaagtagccg cgcatcgtgc
     2341 ccgagcccct gggtgacggg taaggcaggt actcggcgtg gatgcgcgtg tcgtccttcg
     2401 gcacctgctg cgcgagcgca tagtccgggc gcaggctttc gagcatcgcc gccgcgctca
     2461 tcccgccgac cgcatacttc gctgcgcgat cgaggaattc gcgccggccg atctcgccgt
     2521 gcacgtatcc gtcgaagatc gtcagcactt cgcgcggaaa atcaccgacc ttcatccgtt
     2581 tcatcgtgtc gctcctcgta gcgagatcgg ggtcagagct gtaatcgaga tcggggtcag
     2641 agctgtaatt attccgcgaa gagcgcggat gggtcggcat agattgacgc gggaataatt
     2701 acagctctga ccccgatctc gattgttcgc ggaataatta cagctctgac cccgtgccgg
     2761 gcaagacgat cgcgtcttcg ggtgcgaacg cgacgccggc gcgggccccg cgcgcgagcg
     2821 gcgtgtggat gccgccgtcg aacacgagca gctcgcccca tgccgtggcg agcacgtact
     2881 cgatgcggct gccgagatac gcggcgcgcc ggcactcggc cggcagcagg ccgccaccgg
     2941 ccgcggtcaa ccgcagccga tgcggacgca cggcgagcgt cgccgggccg gccggtacgc
     3001 cgtcggcacg caccggcgca ctcacgtcgt tcgcgcgaaa cgaggctcct tcggcgccct
     3061 ggacgagctc cccgctgacc agattcgcat caccgatgaa gtcggcgaga aagcggctgg
     3121 cgggcgcttc gtagagttgc gacggcgtgc cttgttgcgc gatccttccc cgatccatca
     3181 cgatcacctg gtcggagacc gcgagggctt cctcctggtc gtgcgtgacg tagatgaccg
     3241 tgagatcgag cgtctgctgc agctcgcgga tctcctggcg cacgcggcgg cgcagcttgg
     3301 cgtcgaggtt cgacagcggc tcgtcgaaca gcagcaccgc cggctcgagc accagcgatc
     3361 gcgccaccgc gacgcgctgc tgctggccac cggaaagctc cgacggaagc cgctgctcga
     3421 gaccggcaag cccgagcatc cgaagcttcg cgtgtgcgag ctcgcgcgcc ttcgccttgc
     3481 cggccccgct cgaaagcggg ccgtagcaca cgttgtcgag cacgttcatg tgcgggaaca
     3541 gcgcgtacga ctggaacacc atgctgacgt cgcgctcgtt cgccgcgcga tgcgtgacgt
     3601 cgacgccgcc gatcgcgatg cgtccgctcg tcgccggctc gagccccgcc accatgcgca
     3661 acgtcgtcgt cttgccgcag cccgacggac cgagaagcgt cacgagctgc ccgggacgca
     3721 cctcgaagga cacgtcgtct accgccgtga cgtcgttgta gcgcttggtg atgcggtcga
     3781 agacgacccc acccgtaacg ttgctcatcg cgtcccttcc agccttcatt gcggcgccac
     3841 cacgaccgcc gccgcgcgcc gcccgagccg cgcctcgccg acgaaccgct gcatgccaag
     3901 cagcactgcg agcatgaaca gcatcagcac cgtcgagtag acgatggcca gcggatactc
     3961 gccggcctcg acccggttga tgatgtagac cgtcgacagg ttgtatttcg ccgtcgcgag
     4021 gaagatcacc gcggacaccg ccgtcatcgc gcgcgtgaag ctgaacacga gcgtgacgag
     4081 gatcgccgag cgcagcagcg gcatgacgac gcgggtcagc gttcgccacg tcgaggcgcg
     4141 cagcgttgcc gacgcctcgt ccagcgtgcg gtcgatctgg gcgagcgacg cgacgcccga
     4201 ccgcacgccg accggcatgt tgcgaaacac gaagcacagc acgaggatcg ccatgccgcc
     4261 tgtcaattcg agcggtgcga tgttgaacgc cacgatgtac gacacgccga tcaccgtgcc
     4321 gggcacggcg aagctcacga gcgtcaggaa ctcgaacgcg cggcgcccgg cgaaacgatg
     4381 gcgcgtgatg acgtacgcgg acaacaggcc gacgagcgcc gtgagcggtg ccgcgacgag
     4441 cgccacctcg atcgtcgtga acaggctgtc ccacgccgat ccgctgaaca gcagcccgcg
     4501 cgccgcccat tcgaggcgga agccgtcgag gaagtggcgg aacgacggcg tcatgtcgcc
     4561 gcgaccgatg tcgcgcacga agccgccggt gaggatcacc gcgtagcaca tgagcgtgaa
     4621 cacgatgaac gccgccgcga tccagaagca ggtcagccac aggcggcgcg gcagcgcggc
     4681 cggcgcgccg ccgtcgccct tgccgctcac cgtcacgtac gacgcgcggc cgacccagcg
     4741 ctgctgcagc acgaacgcga ggagtgtcag cgcaagcagc acgatggcga gcaccgcggc
     4801 acggcccgcg tcgtactgcg ctcctgcgac ggcgaagaag atcttcgtcg acaggacttc
     4861 gaaattgccg gccagcacga tcggattgcc gaagtcggcg aggctctcga cgaacgcaag
     4921 caggaacgca ttcgcgagcg ccggacgcaa cagcggccag gtgacggtgc gaaacacgcg
     4981 tccgggcgtg ccgcgcagcg tctgcgccgc ttcctcgagc gcggggctga tcgcgtgcag
     5041 cgcgccgtgc agcagcatga acgagatcgg cgtgaacgtg atgagctgcg cgatggccac
     5101 gccgggcagg ccgtagatcc agcgcgagcg ggggatgccg agcacgccga gccagctcgt
     5161 gatgagcccg gtgcggccga acagcacgac gagcgccagc gcaacgacga acggcggcgt
     5221 gatcaccggc agcaccgaca tcagcttcag cacgccggcg tagcgctgcc cgccacgctg
     5281 caccgccagc gcaagcgcca ggcccagcag cgtcgacagc agcccgacga tggttgcaag
     5341 cagcgccgag ttgatcgcga cgccgcagcg cgtgccgccg ccgaggcagc cgagtcccca
     5401 gatgtcgctc gtcaacaggc gctcggcgac gagctgcggc gcgaaatggc cgcgtgcatc
     5461 ctgcacggcg gacgtgagcg cgctgccgat cggatagaag atgaagagga cgagcaggag
     5521 cccgatcgtg acgacgatcg tcgcgatcgt ggcgttgccg gcgaagaggc cgatgcgccc
     5581 gagcgcgcgc gcgaggcaca cggtgattgc ggcgagcgcg atcgtcgcgc cgattccgaa
     5641 cggcggaccc tggaccccgg cgacgaagcc ctgcgcgaac gtccatgcga gggcggccgc
     5701 cgcgaacagc gcggtgaatc gcgtcaagcc gaaccatgcg cacgcgagcg ttgcgatcac
     5761 cgcgagcagc accggaacga gcgccgccga ggcggcgtag cgcggccact tcgcgaggcc
     5821 ggcgccgttg gcgatgccga tcgcactcgc gccgccgtcc atcgcccagg gcagcagcag
     5881 cagcgcggcg agtgccagcg ccgcgaacag cgtcgtcgtc agatccctcg gcgcgcgcgg
     5941 cgcgtgcgct gcgggaagcg ccgccgaggg gagcgacgtg gccactactt cggcagcgtc
     6001 gagacctcct tgtcccagcg tgcgaggagg cgcgtacgct cggccgacga gccgtacttg
     6061 gcgaagtcgt aattgatgag cttgatgtcg gagaacttcg gcgcgagcgg tgacaccggc
     6121 gcgctcttgt tcgacggaag ctggtaggag accttcgcct cgaggccgat cgcctgcgct
     6181 tccggcgtca gtgcccagtc gtaccacttc ttcgcgttgg ccatgttctt cgcgcccttg
     6241 atgatgctca tgctgccgat ctcgtagccg gtgccttcgc acggcgacac gatcttgagc
     6301 ggcgcgccgc cgaggatctg gtacagcgca tcgtgctgga acacgatgcc gaccatcgtc
     6361 tcgccggttg cggcggcacg cgcgggggct gcacccgact tcgtgtactg gttgacgttc
     6421 ttgtgcagcg ccttcaggta gtcgaatgcc ttgtcctcgc ccatgatctg cacgagggtg
     6481 gcgagcatgt tgtacgacgt gccggacgag ttgggattgg cgacctgcac ctcgtccttg
     6541 aattggggct tgacgagatc ggcccagcac atgggctcgg gaacgttctt cttcttcagg
     6601 agctcggtgt tgtacgtata gccgagtgca cccgcataga tgccgacggt gcggtacttc
     6661 gactcctcgg cctggcgcac ggcccagtcc tgcagctgcg cgagcatcgg cgacttgtac
     6721 ggctcggtga ggccttcctc ggccgcctgc agatgcgggt caccggtacc gccccaccag
     6781 atatcgccgc gcgggttcgc cgattcggcc ttgatctgcg cgtagaactc gcccgcgctc
     6841 ttgcgcgtca tcagcacctt gatgccgaat ttcttctcga acgccgccac catcggccgg
     6901 caccattcct cctgcaccgt gcagtagacg acgagctcgc cctgcgctgc ggcggcgagc
     6961 ggcaccgcga agagcaacgc aggcaacagg gcccgcagga acgccgatat gcgcatgctt
     7021 cctcccgatt cgagtcgtgc ggcgggggac aatccccgcg ctcggtcggc gagtgtagcg
     7081 caatcggggc ccgtcaacgc tatgggcgcg aaccctaaag cgatttcaga tattggacga
     7141 ggtcggcgat ctggttgtcc ggcaggccga gcgacttgcg ctggttgtac gtccgcacga
     7201 cgtcctcgag cgtcgccgcg ctgccgttat ggaaatacgg cgcgtgctgc cacaggccat
     7261 gcagcggcgc ggtccggtac tgcttcgtgg cactccgcga tgcgtagctc ggcgcgccgt
     7321 tcggctcggg ctcgctgacc acgtcggtcg gcggatgcag ccgcaggttc gcgtcggtga
     7381 gcgcgtcgcc gctgtgacat gtcgcgcagg tgccggcgcc gttgaaaagc gccttgccgc
     7441 gcgcggccgc aacgggatcg aaactcccgg tgggtggcgg cggtgcgggc agcgacaact
     7501 gataggcctg cagcgccggc agcttcgccg agatgagatc gtcggtaccg ttcgtcacgt
     7561 tgacgccggt gcgtcgatcg gaaaacgtgc cgtgcccgcc catctgtgtc acgccgacat
     7621 agcgattcca gtaggcgatg tcgtcaccgt cgccggtcga cgttatcctg tggatgccgg
     7681 cgaggccgta cgcgggcgga attacctgcg gaccgttcag cccgtcgagg ttgaagcgcg
     7741 gatcgtattt tcccggaccc cagctcttgt agacggcctt gctcgcggcc ggcaccgcgg
     7801 gcgacagcgc gatgatcgcg ccgggatcga ggtcgcgatt gggccagcca tcgagacgct
     7861 tgccgattcc cggcgcgaac gaattgtcga cggtcgagtg gcacagcgcg caggtaatgc
     7921 cgacgcgcgt cagcgtgtcg tgtccgttca ccgtctcgac ggtgcccttg acgccgacca
     7981 ccgcgtcgag cttcagcagc gcgatcgtcg tggcggggct cttgaggtcg atgctgccat
     8041 cctggatgcc ggcaacgacc gctgcgggca gcgcatcggc gtcgaccttg aggccgaccg
     8101 tcagcgcggt cgtcgggtcc accgccgcgc tgatcacttc gtgcatgcgc agcgtatcgg
     8161 tccactgcgt ctcgtcaccg aacgtgtcga aacggaagat ctgcttgccc ttctcgatca
     8221 gcgcgggatc ggtcgcctgc gtggtgtcgg atggcgacga accgccgccg catgcgctta
     8281 ccagcaccgc agccgcgcag gccagcgcgt agccgatcga tctcgcgcgc aatgcgacga
     8341 ccgcgcgacg acacgattcg atcgcggcga ctcgcgaaaa acgcgcgagc gttcccttgg
     8401 cgtacgttcg acgtgccatt tggtgctccg ttggtagtgc gggcgacgag gtgcggcgcg
     8461 atgcagcggg catcgcatgc ggtaatcggc ccattggatg gcgcgcccgc gcgccgcgtt
     8521 cccgtccggt tattcgctgg cgtggaacgc cgaggcaagc agctcgtacg agcgatggcg
     8581 cgccgccgga tcgtgcgtcc acgtgacgac gacgatctcg tcgagcgcga aatgctgcgc
     8641 gagttgcgcc aggcgtgcgg ccactgcgct gccggtgccg acgatcgcct tgcggcgcaa
     8701 cgcatcgatc gtcgcgcgct cgctgtcggt atacggatac gcgtcggcga attcgggcgt
     8761 cacgagcggc ttgcgcaaac ccttctcgaa gccgacgcgc cagaactcgc gcgtcttcag
     8821 aaggtgccgc gcgcaggctt cggtgtcggc cgcaagcgcc catacgcaga tcgtcgcttg
     8881 cggcgccgga tgccttgcac tcggccgata gttgcgccga tagagcgcaa gggcctggtc
     8941 ggcgccgcgg ccctcggtga agaaataggc aaacgcgtac ggaaggccga aatgcgcggc
     9001 aagctgcgcg ccgtaatccg agctgccgag gatccataac tcgggacacg tgggcccgcg
     9061 ggggttggcg ctgatcgcgg cgaacgggtg gccctcgtcg agcggctcgc cgcagaccca
     9121 ggcacgaagc tcgtgcacct ggcgggggaa ttcctcggcg gcgttcgaat acggattgag
     9181 cgcgagcgcc gtaaggcgat cggagccggg tgcgcgcccg acgccaaggt cgatgcgtcc
     9241 gggcgcgatc gcttccagca cgcgaaactg ctcggcgact ttcaacgccg agtaatgcgg
     9301 cagcatgacg ccggcgctgc cgacgcgaat tcgcgacgtc gtcgcagcga tcgccgccat
     9361 cagcacttcc ggcgcggtgc cgacgatgct gtcgctgctg tgatgctccg acacccagta
     9421 gcggtgatag ccgagcgcct cgcaatggcg cgcgagcgcc agcgtttcct ggatggcggc
     9481 gtgctggctc gcgccttcgg cggccgtcga ctggtcgagg accgagagtt tcagggggtg
     9541 agcacgacct tgatgcagtc gtcctgcttg ttcgcaaaca tcgcgtaacc gtcgggtgcg
     9601 tcatcgaggt gcatgcggtg cgtgacgatg aagctcggat cgatctcgcc gctttcgatg
     9661 cgcccgagca gcggcttcat gtagcgctgc acgtggcact gcccggtctt catcgtgagc
     9721 gagcgattca tgaacgaccc catcgggaac ttgtcgatga acccgccgta gacgccgatg
     9781 accgaaaccg tgccgccatt gcggcacgcc acgatcgcct ggcgcagcgc gatcggccga
     9841 tccgattcga gcatcagcgc ctgcttgaag cgatcgtacg cgtacatgat gccgggcgca
     9901 tgcgcttcgg tgccgaccgc gtcgatgcag gcgtcgggac ccacgccgcc ggtgagttcc
     9961 ttcagcgtgt cgagcacgtc ctggtcctcg tagttgatcg tgtcggttgc accggccttg
    10021 ttcgcggcca gcgcgaggcg ctccggcacg cggtcgatcg cgatgatgcg cgcggcaccc
    10081 agcagcctcg cactcgcgac cgcaaattga cccaccggac cgcagcccca gacggcaatc
    10141 gtatcgcccg gcgcaatgtc gcacatcttt gccgccatat agccggtagg cagcacgtcc
    10201 gaaagaaaca gtgcctgttc gtcgctgatg ccagcgggca ccttgagcgg cccgacgtcg
    10261 gcgaacggca cacgtgcgta ttcggcctgt ccgccggcga atccgccgag catgtgcgag
    10321 tagccgaaga tgccggccgg cgaatgtccg agcagcttct ctgccatcca tgcattcgga
    10381 ttcgagttct cgcacagcga gtacgcctgc gcctcgcacg cgccgcagtt gccgcatgcg
    10441 atggggaacg gaacgacgac gcggtcaccg accctgaggt tcttcacgtc gcggccgacg
    10501 tcgacgacct cgcccatgaa ctcgtgtccc aggatgtcgc cctttttcat cgtggggatg
    10561 aagccgttgt aaagatgcag gtcggagccg cagatcgcgg tggacgtgac gcgcacgatc
    10621 gcgtcgcgct gattcaggat gacggggtcg ggcacgctgt cgacccgcat gtcgcgtttg
    10681 ccgtgccagc aggctgctct catcgtggtc gcgcctccga cgcttgcccg ggctgctgct
    10741 tcacgtcgcc cacgccatgc gggctgccgt cggagcgcac gatctcgccg gtctcgacga
    10801 tctgcttgaa gcggtgcagg tcgtcgtaga tctgctgctc gggtgattcg ttgaagagca
    10861 tcgcggcgag cctgcccgcc atgccgccgg gcggccggta caggacatcg acgcgcacga
    10921 tcgtgccgcg gttgccggga cgcggctcga agcgcacggt cccccggctc tcgacgccag
    10981 caccttcgag cgactgccag gcgatcagct cgttgggcgt atccgcggtg atgcgtgcgt
    11041 cccattcgat ggtgctcccc gccgggccac cgaccgtcca gtgcgacagt ccggggccga
    11101 tcgcgcgcac cgaattcacg tgatacatga aacgcggaaa gttctcgaac tcgcgccaga
    11161 acgcgtacaa ctcgtgcggt gggcgattga cggccacgct tcgcgtcatc cggatagcct
    11221 cctcgccgcg tcgattgcgc gtcagcccgc gtgcgcaata caggtcgagc gcggtgacgc
    11281 cgagcacgcc ggcggccgcg acagcgacgc cactcctgtt catccgcgag gacgcgccgg
    11341 ccgccgcgag cgtcgcgatg tcgaccgcat cgcccgccac gcgcgaccag actgccgccg
    11401 cgggccgggc tccctggccg aagatcatga gtccgcttac cagctcgcgc aacccgtaca
    11461 ggcggatcag attcgtgtac ttgccgttgc cgccgacaag tcgcgacaca agccgcggcg
    11521 cgagcatttc ggcgaggccg agcgagatgc tgaaccaggc gagaccgtgg gcgaggcgct
    11581 cggcgcctgc ctcgccgagc tgtcgcgatt cgtcgcggct ggcgtgagcg gcatggcgag
    11641 ggcggcgcat cacttccggc attgcatcgt cgcgcggccc gggcggccag tcgtcggttc
    11701 cttgcatggc gatctcctct tccgaatgcg cgagcgaggc gcatgcgtgg tgtgacacgc
    11761 acgccttgcc ttcggttgcg agaagcgtgc cccgcgaacg attatgcgat cgacatgatc
    11821 accggcgcgt ggtcgctggt gccgacgtcg gcgagcacct tgcattcggc gacgcgatcg
    11881 gcgatcgagc gcgacgcgag gatatagtcg atgcgccagc cgatgttgcg ctggcgcatg
    11941 ttgcgccacg gtggccacca cgagaacatc gcttcatcgt cgggatgcag cgcccggccc
    12001 acgtcgacga agtgcccgcc gagcagccgg tcgaaaagct cgcgttcctc cggccgctgc
    12061 ccgatcacgc ccgccttgcg ctcgcgcgga tgcacgtcga tgtccgcgtg cgcgacgttg
    12121 agatcgccgc acaagacgac ctcgcgccct tcggcatgaa gccgcgccgc ccagtcggcg
    12181 agcttcgaca ggaacgcgat cttcgcctga tagtccttgc cgccgttcgg cacgtaaacc
    12241 gaagcaacga cgaggtcgcc gagcgctacc tgcacgatgc gcgattccat gtcgaaatcg
    12301 ggatgcgcga acgccggcgc gtcaccgtgc aacgccttgt tgacgtgcag cgacacgccc
    12361 gagtagccgc gcatgccgtg ccagtagacg tgataatcgg cgtgccggca gcgctcggga
    12421 atctgcgccg gctcggcctt gagctcctgc aggcacacga cgtcggggcg gtcgcggtcg
    12481 agccagtcgc aaaactgcgc ttcgcgtgcg cgaattccgt tgacgttcca tgtcgcgacc
    12541 aacatcgtct gccctcgcct gcccggggtc gctagcgcct gcgcgcgagc gtcaatccat
    12601 cgccgatggg cagcaacgac agatcgatgc gctcgtcgcg atgcagcttg tcgttcagcg
    12661 cctgcagcgc ggcggtgtcg gccgtcgtcg ccggctcggc gacgctgccg ccccacagaa
    12721 cgttgtcgat cgcgatcagg ccgcccggcc gcaccagcat gagacaacgc tcgtaatagt
    12781 cgtcgtatgc cgtcttgtcg gcgtcgatga acgcgaagtc gaacgcgttc gcctcgccgt
    12841 tggcgagccg cgcgtcgagc gtcgcgcgtg ccggcgcgag cgtcagatga atcttgtgcg
    12901 cgacgccggc cgcttcccag tacggcttgc cgatgcgcgt gtactcgtcg ctcacatcgc
    12961 acgcgagcac acggccatcg tccggcaacg ccagcgccac gctgagcgcg ctgtagccgg
    13021 tgaagacgcc gatctcaagc gtgcgccgcg caccgatcag ccgcacgagc aacgccatga
    13081 actgaccctg ctcggggccg atctgcatgc cggcgtgcgg gtgcgccttc gtcgcctcgc
    13141 gcaatgcggt ctgggccgga tgctcgcgca gcgaatgcgc cagaacgtat gcgtacaacg
    13201 agtcgtcgag attcagcgtg cggtttgcca tacgtcccct ggaagtcaaa cgcggtgcga
    13261 ggcgccgcga aggcgcgcgc accgcgcatc gtcgacgtgc gtgcgcctca gcgtcgcatg
    13321 cggcccgcga tgttcgccat ggcgatcgtg atcgcggcgg cgccgagccg cttcacgaga
    13381 tcgggatgct gcgcgtagaa gttgccgagc gcgtcgaaaa cgcccgaccc atgctgctcg
    13441 gcgtgattcg cgagatcctg cacctgcgac ggagagatct gcgacgcctg ctgcggggtg
    13501 acctgcggcg tgccgccgcc ggtcaactgc ccgagcagtc cgccgagcgc acctccgccg
    13561 agcgacgaca gcgctccgcc gcccagcgag gacagcgcgc cctgcggcag cgaagcgaga
    13621 atctggttca gcatcccggc ctgctgattc ggatccgcct tcgaatacac gtcgctcacc
    13681 atctgcccga gcgggggcgt tgcgtcagag cgcagcgcgt cggaaatgcc ctggcccaac
    13741 acctcgggag atgcatgctg cgcgacctgg tcgaaatgtt cctcggcgcc gggcgccgcc
    13801 tgtccggccg gcacgttcag atacttctgc agcagtcctc cgagatcgaa tgacatgggg
    13861 cctcctcagt ctacggcttg taccacgcgg ggaattcctt cttcagctcg gcgagcttcg
    13921 gcaggtcgtt gtagacgatg tacggctgct cgggatgcag cgccaggtaa ttctgatgat
    13981 aatcctcggc cgggtagaac ttgtcgagcg gcgcgacctg cgtgacgatg ggcttgccga
    14041 atgccttggc cgcgtcgagc tgcgcgatgt aacgctcggc gagctccttc tgctgtgcat
    14101 tcgcatagaa gatggccgag cgatactgcg agccgacgtc gggaccctgc ctgttgagct
    14161 gcgtcggatc gtgcgcgacc gagaagaaca ccttcaagag ctcggcgtaa tcgactttcg
    14221 acgggtcgta cgtcaccttc accgattcgg cgtgcccggt ggcgccggtg ccgaccatca
    14281 tgtacttcgc ggtggcagcg ctgccgccgg aatagcccga cacgacgttg gtgacgccct
    14341 tgacatgctt gaacacggcg tcgacgcccc agaagcatcc gccggcgaag accgcggtgg
    14401 cctcgcccgg cgtgctcgcc ttcgacacgg ggccggcgta atcgggaacc gcggcgtgcg
    14461 cgccgagcgc ggcgaacacc atcatgaaaa acaggttgcg catggagacc tccttcgtgg
    14521 gggacacgcg caatgagttc gcctggcgcg tccgatcctt acgacgttgc gacgaacggc
    14581 aagttctgca agccggccgg cgacccgccc cctgtcatcg agccgaccac gataccaccg
    14641 caccgccggg cctacttggt gccgaagatc cggtcgccgg cgtcgccgag gccgggaagg
    14701 atgtaaccgt gcgagtcgag ctcgcgatcg atcgcggcgg tgtagaccgg cacgtcggga
    14761 tgcgcctcct ggaacgtcgc gatgccttcg gggcacgcga cgagacagac gatgcggatc
    14821 gagcgcggcc gcgtctcctt cagccgctcg accgccgcga ccgccgaatg gcccgttgcg
    14881 agcatcggat cgagcacgat cgcgtcgcgc tcgtgcatgc cgcccggcag cttgaaataa
    14941 tattcgacgg cgacgagcgt cttcggatcg cggtagaggc cgatgtgccc gacgcgcgcg
    15001 ccgggcacga cgttcaggaa gccgtcgagc aaaccggtgc cggcccgaag gatcggcacg
    15061 aatacgagct tcttgccgtc gatgagccgc gagcgcatcg tctcgagcgg cgtctttatt
    15121 tcgacctcgt tcatcggcgc atcgcgcgtg acctcgtagg ccatcagcgc gccgatctcg
    15181 ttcaggagcc ggcggaagtt ggtcgtgctc gtttccctgc gccgcatcag cgtgagcttg
    15241 tgctggatca gcgggtgatc gagcacgtgc acggttgccg ccgcttccgg catcagaaga
    15301 cggtgccgtc cgacaacacg cgcagcggcg gctcgccgtt gcgcaggcgc atcgcgagct
    15361 cgcggcccgc ggcccaggtc ccgccttcca gaatgcaggc gagcggcaat tgcggccggt
    15421 cgagctcggc acgcacgcat tgtgcaagct catcgagcag cgtgacggtg agtgcgcgcc
    15481 actcgacgac gagctcgctt tccggcgccc actgcgcccc gcgatcgacg caggaatcga
    15541 gcttcagcac gccggtgtcg atcagcaggc cgccgttgcg gtattcgggc aaagcggtca
    15601 gcgcgtcgcg atcggtgacc gggatgcccg cccattcgaa cggctcgaac agcgaatacg
    15661 cgagccattg cg
//
LOCUS       DASSVX010000067         7493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_819062, whole genome shotgun sequence.
ACCESSION   DASSVX010000067 DASSVX010000000
VERSION     DASSVX010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819062"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFO53_03110"
     CDS             complement(<1..737)
                     /locus_tag="VFO53_03110"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875294.1"
                     /translation="MLLVVVVALAAIAWGAVAGAKPEGQRPPVRVMLVDAGWYAALPA
                     DPAAATKAFIDRVPADARASAEAYNRQSVAAIVLRIVVLFVVTTLTMMSGLAARFRDN
                     ARRLTRRVFLQDALVVVQFIALAMAAGLPVEIFAGFVRVRAAGLSHIAFWPWLTDYLL
                     RWTVDLVFYVIGIAAIMALIRRLPRAWPALATLVYVALYATYTLISPVFIEPLFNVFA
                     QLREGPARERILSLARANGIDTDGVYI"
     gene            complement(779..1792)
                     /locus_tag="VFO53_03115"
     CDS             complement(779..1792)
                     /locus_tag="VFO53_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8875295.1"
                     /translation="MEYRRLGLSGLEVSIVCLGTMMFGDRTDAADANAIVAHAFEHGI
                     NFIDTADVYANGASETITGAAIRGQRSKWILATKVGNPMAGDGNARPHTGGLSRRWIM
                     QACEASLARLGCEFIDIYYLHIDDAKTPLAETVSAMGALIDAGKIRYFGISNFRGWRI
                     AELMHWCERLGVPPPVVCQPYYNLLNRMPEVEVLAACDHYGIGVASYSPVARGVLTAK
                     YAPGNVPADSRAARNDRRLMQTEFREESFAIAQTLKQHAERTGRTPVQFALAWLWANR
                     IVTSVIAGPRTLAQWTDYVDAIGAPWSADDEALVDSLVRPGHPSTPGYSDPQYPIYGR
                     RLD"
     gene            complement(1853..3001)
                     /locus_tag="VFO53_03120"
     CDS             complement(1853..3001)
                     /locus_tag="VFO53_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HET8875296.1"
                     /translation="MRTSTERFLTTHTGSLPRPDDLVRMMYAKEEGVPVERHALAARV
                     ESAVADVVARQVKAGVDIVNDGEMSKPSYATYIKDRLSGFGGEGNTFVYQDLADFPKL
                     EKRVFGDPGRSRRKTPACNAAIGVRDPQAARTDIAHLSTAMKTAGATDGFLTAASPGV
                     VSLFFRDEHYGSEEAYLAAIADAMRDEYEAIAAAGFVLQIDCPDLGMGRHIQFASLSL
                     PEFRKKAELHVEALNDALRNIPPEQLRMHLCWGNYEGPHHCDVPLADIIDVVFRARPS
                     AIALEAANPRHAHEWKLFETVELPEGKVLIPGVIESKSNFVEHPELIAQRIGRYANLV
                     GRENVMAGSDCGYGTWVGQAAVDPDVVWAKMAALAEGARIASREFWKR"
     gene            3149..4996
                     /locus_tag="VFO53_03125"
     CDS             3149..4996
                     /locus_tag="VFO53_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HET8875297.1"
                     /translation="MNVLFEDDGQLKAGTLLADQDASLQVEAISGKRQKIKAAHVLLR
                     FAAPSAAETMAKAHQIVADIDTQFLWEVSVDDEFGFDELAREYYGAAPAPAEAAAVAL
                     ALSRAPMYFYRKGRGRYRRAPPDALKAALASVARKEREARDIAQWSAELRAHRLPAAL
                     AAKLDMLLYRPDKNAPEWKALDAACAAERISAADLLRACGAIPSSHDYHFNRFLTETF
                     PQGVEFPAWDPLPPTPELPVAPVRAFSIDDASTTEIDDAFSVRDLGDGHREIGIHIAA
                     PALLIAPGTALDAIARRRLSTVYMPGRKLTMLPERVVEALTLREETTPPALSLYVETG
                     PDGAPLRHRTALERVPIAANLRLATLTEAFARDAAADDPPWTAELRALWQLAQHVAAA
                     RGKPDVARTDFTFDVDWTREGAGERGRVTIAARPRGSPLDKLVAELMIHVNHVWGQRI
                     ADAGIPGFYRVQAGGKVKMSTRPGEHQGLGVSHYLWSSSPLRRYSDLVNQRQLIATVR
                     NERAPYVDGDAELFAALADFEATYSQYAELQDRMERYWCLRWLLQEHVSECTATIIRD
                     NLVRFDSLPLVVRLPDLPAGTPGAGVRVAIGRIDLLDATLECRYAGAAG"
     gene            5124..6173
                     /locus_tag="VFO53_03130"
     CDS             5124..6173
                     /locus_tag="VFO53_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET8875298.1"
                     /translation="MPQPVAQPKLSAVRTDRDAVVPARGTSAKPQPERLATAKPASQR
                     KGNLRFAGVLPPARIPPESIAPARRVSAIGIAVGVSIALHAIAMTIHFTFGSGRFTND
                     AAPLQIALVNARSASKPAKADILAQANLDGGGNTEKDRRAKTPLPVLPNDMPRQNVAV
                     ASSRADAQENVTREMLTQLRSGVVVPVPEPKPVDESERTDSPTANELMQRTLEAMRLE
                     AQIARDMDAYQKIPRRKFIGAQAKEYRFARYVEDWRMKVERIGNTNYPEAARQNRLYG
                     SLILSVSIRADGSIENVEVKRKSGQRVLDAAAVKIVEMAGPYAPFPDDIRRDTDILNI
                     TRTWTFAKGDELHSD"
     gene            6268..6963
                     /gene="mtgA"
                     /locus_tag="VFO53_03135"
     CDS             6268..6963
                     /gene="mtgA"
                     /locus_tag="VFO53_03135"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HET8875299.1"
                     /translation="MKRVWRVLGVVVGFVALAVVLVHLYFAAMIVWWRDHPPRETSFM
                     ADRMRELERKTPGAPLRYQFVPYERVAVSLKRAMIAAEDSKFVEHEGFDWDGIELAIE
                     KNQKRGRIVAGGSTITQQLAKNLFLSGARSWFRKGEEAVITLMLEALLDKRRIFELYL
                     NVIEWGNGVFGAEAAAQRYFRTSAARLSPEQAARLAAMAPNPRFYERNPGAPGLGRKI
                     QIILARMPAAELP"
     gene            6993..7481
                     /locus_tag="VFO53_03140"
     CDS             6993..7481
                     /locus_tag="VFO53_03140"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875300.1"
                     /translation="MPESETVADRPEPREHVEDALAEVTALLRKNRLVEGLIHEQFGH
                     APDAARDELRQSAVARENKAALERTLARLHAADIAYVLEALPLDERLYIWDLVKADRD
                     GEILVEASDAVRESLISAMDTEELVAATEALPADEIAALAPDLPQEVMDDVFRSLPVA
                     ER"
BASE COUNT         1301 a         2518 c         2439 g         1235 t
ORIGIN      
        1 ttgatgtaca cgccgtcggt gtcgatgccg ttcgcgcgcg ccagcgacag gatgcgctcg
       61 cgtgcaggcc cttcgcgaag ctgcgcgaac acgttgaaaa gcggctcgat gaagaccggc
      121 gatatcagcg tgtacgtcgc gtagagcgcg acatacacga gcgtcgccag tgctggccac
      181 gcgcgcggaa ggcggcggat caacgccatg atcgccgcga tcccgatcac gtagaaaacg
      241 aggtcgacgg tccagcgcag cagatagtcg gtcagccacg gccagaaggc gatgtgcgac
      301 aggccagccg cgcgcacgcg gacgaatccc gcgaatatct cgacgggcaa tcctgccgcc
      361 attgcaagcg cgatgaattg cacgacgacg agcgcgtcct gcaggaacac ccgccgcgtc
      421 agccgccgcg cgttgtcgcg gaagcgcgcg gccaggccac tcatcatcgt cagcgtcgtc
      481 acgacgaaca gcacgacgat tcgcagcacg atcgccgcga cgctttgccg gttgtatgcc
      541 tcggcgctcg cgcgcgcgtc ggcaggtaca cggtcgatga atgctttcgt cgccgccgct
      601 ggatcggccg gcagcgccgc ataccagccg gcgtcgacca gcatcacgcg aacgggcgga
      661 cgctggccct ccggtttcgc gcccgctact gcaccccacg cgattgccgc gagtgctacg
      721 actacgacga gcagcacgac gccgcggccg gcgcccgccc gcgttggcct tgcgtcgatc
      781 aatccagcct gcgaccgtag atcggatact gcgggtcgct gtaccccggt gtcgatggat
      841 gaccgggacg gacgagcgag tcgacaagcg cttcgtcatc cgcgctccac ggcgccccga
      901 tcgcgtcgac gtagtcggtc cactgcgcaa gtgttcgcgg gccggcgatc accgatgtga
      961 cgatccggtt cgcccacagc cacgcgagcg cgaactgcac tggcgtgcgc ccggtgcgct
     1021 cggcgtgctg cttcagcgtc tgcgcgatcg caaacgactc ttcgcgaaac tcggtctgca
     1081 tcagccgccg atcgttgcgg gcggcgcgcg agtcggcggg cacgttgccg ggtgcgtact
     1141 tcgccgtcag cacgccgcgc gccaccggcg aatacgaagc gacgccgatg ccgtagtgat
     1201 cgcacgcggc gagcacttcg acctcgggca tccggttcag caggttgtaa tacggctgac
     1261 agacgacggg cggcggaacg ccgagacgct cgcaccagtg catcagctcg gcgatgcgcc
     1321 agccgcggaa attcgaaata ccgaaatagc gaatcttgcc ggcatcgatc aacgcgccca
     1381 ttgccgagac cgtttccgcg agcggcgtct tcgcgtcgtc gatgtgcagg tagtagatgt
     1441 cgatgaactc gcagccaagc cgcgcgagac tcgcttcgca cgcctgcatg atccagcgtc
     1501 gcgacaggcc gcccgtgtgc ggtcgcgcat tcccatcacc ggccatcgga ttgccgacct
     1561 tcgtcgcgag gatccacttc gaacgctgcc cgcggatcgc cgccccagtg atcgtttccg
     1621 acgcaccgtt cgcgtagacg tccgcggtat cgatgaaatt gattccgtgc tcgaatgcgt
     1681 gcgcgacgat cgcgttcgca tctgctgcat ccgttcgatc gccgaacatc atcgtgccga
     1741 ggcacacgat cgacacctcg aggccgctca acccaagtcg ccggtattcc attgggatgt
     1801 ccttttgcgc cggcacatgc gacgcgccgt gttgatcgat gcaaacccga cgttaccgct
     1861 tccagaattc gcgcgatgcg attcgggctc cttcggcaag cgccgccatt ttggcccaga
     1921 cgacgtcggg atcgaccgca gcctggccga cccatgtgcc gtagccgcaa tcgctgccgg
     1981 ccatgacgtt ctcgcggccg acgagattcg cgtagcggcc aatcctctgc gcgatcagct
     2041 ccggatgctc gacgaagttc gatttcgact caatcacgcc ggggatcagc accttgcctt
     2101 cgggaagctc gaccgtctcg aacagcttcc attcgtgtgc gtgacgcgga ttcgccgcct
     2161 ccagcgcgat cgccgagggt cgcgcgcgaa acacgacgtc gatgatgtcc gcaagcggca
     2221 cgtcgcagtg atggggccct tcgtagttgc cccagcacag atgcatgcgc agttgctcgg
     2281 gcgggatgtt gcgcagcgcg tcgttcaacg cctcgacgtg cagctcggcc ttcttgcgaa
     2341 attccggcag cgacagcgac gcgaattgaa tgtgccggcc catgccgaga tcgggacagt
     2401 cgatctgcag cacgaaaccc gcagccgcga tcgcttcgta ctcgtcgcgc atcgcgtcgg
     2461 caatcgcggc aaggtaggcc tcttcgctgc catagtgctc gtcgcgaaag aacagcgaca
     2521 cgacaccggg cgatgctgcc gtcagaaatc cgtcggtcgc acctgctgtc ttcattgcag
     2581 tggaaagatg cgcgatgtcg gtgcgcgcgg cttgtggatc gcgaacgccg atcgccgcat
     2641 tgcacgcggg cgtcttgcgc cgcgaccggc cgggatcacc gaacacgcgc ttctcgagct
     2701 tcgggaaatc ggcgagatcc tgatagacga acgtattgcc ttcgccgcca aaacccgaca
     2761 accgatcctt gatgtaggtc gcgtagctcg gcttgctcat ctcgccgtcg ttgacgatgt
     2821 cgacgccggc cttcacctga cgggcgacga catcggcgac ggccgactcg acgcgcgctg
     2881 cgagcgcatg gcgctcgacc ggtacgcctt cctctttcgc atacatcatc cgcacgagat
     2941 catcgggccg cggcaagctg ccggtatgcg tcgtcaggaa ccgctcggtg ctcgtgcgca
     3001 tgagatttcc cagtaggcgt cgaccgattg tcgcatgccc tcgacgcgcc gattgtggac
     3061 atttcggcgt gaatccgccg tgcttgtaac gcggacgccg cacgagcgcg ggttacaatc
     3121 agcgcccgtt ttgcggtaac cgatacgcat gaatgtgctg ttcgaggacg acggtcaact
     3181 gaaggccggc acactcctcg ccgatcagga cgcatcgctc caggtcgagg cgatttccgg
     3241 caagcggcag aagatcaagg cagcgcacgt gctattgcgc ttcgccgcgc cgtccgctgc
     3301 tgagacgatg gcgaaggcgc accagatcgt cgccgacatc gacacgcagt tcctgtggga
     3361 agtgtcggtc gacgacgaat tcggtttcga cgagctcgcg cgcgagtact acggcgccgc
     3421 accggcgccc gccgaagcgg cggccgttgc actcgcgttg tcgcgcgcgc cgatgtattt
     3481 ctatcgcaag ggacgtggcc gttatcgccg tgcaccgccc gacgcgttga aagcggcgct
     3541 ggcatccgtc gcccgcaagg agcgagaggc gcgcgatatc gcgcaatgga gcgccgagct
     3601 gcgtgcgcac cgtttgccgg ccgcgctcgc cgccaagctc gacatgctgc tgtaccggcc
     3661 ggacaagaac gcgcccgaat ggaaggcgct cgacgccgcg tgtgcggccg aacgcatcag
     3721 cgccgccgat ctgcttcgcg cgtgtggcgc gatcccgtct tcgcacgact atcacttcaa
     3781 tcggttcctg accgagacgt ttccgcaggg cgtcgaattt ccggcgtggg atccgttgcc
     3841 gccgacgccg gagctgccgg ttgcgccggt gcgcgccttc tcgatcgacg atgcgtcgac
     3901 gacggagatc gatgatgcgt tctcggtgcg cgacctcggt gacgggcatc gtgagatcgg
     3961 cattcacatt gcagcgcccg cactgctgat cgcgccgggg accgcactcg atgcgattgc
     4021 gcgccggcgg ctgtcgacgg tttacatgcc gggacgcaag ctgacgatgc tgcccgagcg
     4081 ggtcgtcgag gcgctgacgc tgcgcgagga aacgacgccg ccggcgctgt cgctctatgt
     4141 cgaaacggga ccggacggcg cgccgctgcg ccatcgcacg gcgctcgagc gcgtgccgat
     4201 cgcggcgaat ctccgactgg ccacgctgac ggaagcgttc gcgcgcgacg ctgccgcgga
     4261 cgatccgcca tggaccgcag agcttcgcgc gctgtggcag cttgcgcaac acgtcgctgc
     4321 cgcgcgcggc aagcccgacg tcgcgcgcac cgatttcacg ttcgacgtcg actggacccg
     4381 ggagggcgcc ggtgagcgcg gccgtgtgac gatcgcggcg cgtccgcgcg gcagcccgct
     4441 cgacaagctc gttgccgagc tgatgattca cgtcaaccac gtttggggac agcggatcgc
     4501 cgatgcggga attcccggct tctatcgcgt gcaggcgggc ggcaaggtga agatgagcac
     4561 gcgtcccggc gagcaccagg gactcggcgt cagccattac ctgtggtcga gctcgccact
     4621 gcggcggtac agcgacctcg tcaaccagcg gcaacttatt gcgacggttc gcaacgagcg
     4681 ggcgccgtac gtcgacggcg acgccgagct ctttgccgca ctcgccgact tcgaggctac
     4741 gtattcgcag tacgcggagc tgcaggaccg gatggagcgt tactggtgcc tgcgctggct
     4801 gctgcaggag catgtgtccg agtgcacggc gacgatcatc cgggacaatc tcgtccggtt
     4861 cgattcgttg ccgctcgtcg tccggctgcc cgacctgccg gcggggacgc ccggcgccgg
     4921 cgttcgcgtc gccatcggcc gcatcgacct cctcgacgca accctcgaat gccgttacgc
     4981 cggcgccgcc ggctgaaacc tccgccaaaa ccgttggaat gttgccacgc ggcgcttcac
     5041 ggctgatcaa tggccggccc gcaacgctat gacgacggca tgaccgcgct agaatcaggt
     5101 tgagccaatc cgcacgctac gccttgcccc agcccgtcgc ccagccgaag ctgtccgcgg
     5161 tccgaaccga ccgcgacgcc gtcgtccccg cgcgcgggac gtctgcgaag ccgcagccgg
     5221 agcggctggc gacggcgaag cccgcgtcgc aacgcaaagg caaccttcgc ttcgccggtg
     5281 tgctgccgcc ggcgcggata ccgcccgaat cgatcgcgcc ggcacgccgc gtgtcggcga
     5341 tcggcattgc cgtcggcgta tccatcgcac tgcacgccat cgcgatgacg attcacttca
     5401 cgttcggatc gggccgtttc accaacgacg cggcgccgct gcaaatcgcg ctcgtgaatg
     5461 cgcggtccgc atcgaagccg gcgaaggccg acatcctcgc gcaggcaaat ctcgatggtg
     5521 gcggcaacac ggaaaaggac cgccgcgcga agactccgct gcccgtgctg ccgaacgaca
     5581 tgccgcggca gaacgtcgcc gtcgcatcct cgcgcgccga cgcgcaggag aacgtgacgc
     5641 gcgagatgct gacgcagctt cgctccggcg tcgtcgtgcc ggttcccgag ccgaagcccg
     5701 tcgacgagag cgagcgcact gattcgccca cggccaatga gctgatgcag cgaacgctgg
     5761 aggcgatgcg gctcgaggcg caaatcgcgc gcgacatgga cgcgtaccag aagattcccc
     5821 gccgcaagtt catcggtgcg caggcgaagg aataccggtt cgcgcgctat gtcgaggact
     5881 ggcggatgaa agtcgagcgg atcggcaaca cgaactaccc ggaagcagcg cggcaaaacc
     5941 ggctctatgg cagcctgatc ctgtccgtgt cgattcgcgc cgacggcagc atcgagaatg
     6001 tcgaggtgaa gcgcaaatcc ggccagcgcg tgctcgacgc cgcggcggtg aaaatcgtcg
     6061 agatggcggg tccctatgcg ccgtttcccg acgacatccg ccgcgatacc gacatcctga
     6121 acatcacgcg cacgtggacg ttcgcgaagg gtgacgagct ccatagtgat tgaccgggct
     6181 cttacgacgg cgccggtcat cgctgtgaaa cgctagggcc tgcggccccc aagcgttcaa
     6241 ccatccacag tcaccaggaa gccaccgatg aaacgcgtgt ggcgggtgct tggtgtcgtc
     6301 gtcggcttcg tggcactcgc cgtcgtgctc gtccacctgt atttcgcggc gatgatcgtc
     6361 tggtggcgcg accatccgcc gcgcgagacg tcgttcatgg ccgatcgaat gcgtgagctc
     6421 gagcgcaaga cgccgggtgc tccgctgcgc tatcagttcg tgccttatga acgcgtcgcg
     6481 gtatcgctga agcgggcaat gatcgcggcc gaggactcga aattcgtcga gcatgaaggc
     6541 ttcgattggg atggcatcga gctcgcgatc gagaagaacc agaagcgcgg ccggatcgtc
     6601 gccggcggct cgacgatcac gcagcaactt gccaagaacc tgttcctgtc gggcgcgcga
     6661 tcgtggtttc gcaaaggcga ggaagccgtc atcacgctga tgctcgaggc gctgctcgac
     6721 aagcgccgca tcttcgagct ttatctcaac gtcatcgagt ggggtaacgg cgtgtttggc
     6781 gccgaggccg ccgcacagcg ctatttccgc acgtcggctg cgcggctctc gccggagcag
     6841 gcggcgcgac tcgccgcaat ggcgcccaac ccgcgcttct acgaacgcaa tcccggtgcg
     6901 cccgggctcg gccgcaagat ccagatcatt ctcgcgcgaa tgcctgccgc cgagttgccg
     6961 taggcggccc gcgccccgat actcgcctgg tgatgccaga aagcgaaacc gttgccgatc
     7021 gccccgagcc gcgcgagcac gtcgaggacg cgctcgccga agtcaccgcg ctcctgcgca
     7081 agaaccgact cgtcgaaggg ttgatccacg agcagttcgg gcacgcgccc gacgcggcac
     7141 gcgacgagtt gcgccaaagc gccgtcgcgc gagagaacaa ggcggcgctc gagcgcacgc
     7201 tcgcgcggct gcacgccgcc gacatcgcgt acgtgctgga ggcgctgcca ctcgacgagc
     7261 gcctctacat ctgggatttg gtcaaggcgg atcgcgacgg cgagatcctc gtcgaggcgt
     7321 ccgatgcggt gcgcgagtcg ctgatctcgg cgatggacac cgaggaactc gtcgcggcga
     7381 ccgaggcatt gcccgccgac gaaatcgctg cgctcgcgcc ggacctgccg caggaggtga
     7441 tggacgatgt gttccgcagc cttccggttg cggagcgcta gcagttgcgc gcg
//
LOCUS       DASSVX010000068         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_635871, whole genome shotgun sequence.
ACCESSION   DASSVX010000068 DASSVX010000000
VERSION     DASSVX010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635871"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFO53_03145"
     CDS             complement(<1..635)
                     /locus_tag="VFO53_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET8875301.1"
                     /translation="MPSVEVKVPDIGDFKDVPVIDVLVKPGDTVEPEDALVTLESDKA
                     TMDVPSPAAGTVKAVAVKVGDKVSQGSVVLTLEGEPGAGQPAEAKRASAAPAPPTATA
                     APAKPAAPAAQASKPAAQTPQPAAGPATFTGRADVECDMLVLGSGPGGYSAAFRAADL
                     GMRTVLVERYATLGGVCLNVGCIPSKALLHTAFVMDEVKTMAAHGISYAPPA"
     gene            complement(667..2049)
                     /locus_tag="VFO53_03150"
     CDS             complement(667..2049)
                     /locus_tag="VFO53_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HET8875302.1"
                     /translation="MTTREPAAPRAGKASVSPPPASAQSPAGIAEVRVPDIGGFRDVP
                     VIEIMVKPGDVVEAEDSLVTLESDKATMDVPSPLGGTVRELRVAVGDRVCEGTTIATL
                     TTEVDAMQAPASPAAPQAQRPEFAAPPKTPEARHTRSTAAPASDAAPIALPYASPAVR
                     RRARELDVDLVHVSGSGRNGRIVREDVEAFAKGVVHAAVPPRDARSDQSVHAARTSVD
                     VLPWPKVDFAKFGPIEAKPLSRIRRLSGANLARNWVTIPHVTNHDDADVTELEAFRIE
                     LNGEIAKSGVRVSMLAFLIKAACATLAAFPEFNASLDGDELILKRYYHIGFAADTPDG
                     LVVPVIRDADRKGIVDIAKEAGELATLARNGKLGADRMQGGTFTISSLGGIGGTYFTP
                     IINAPEVAIVGVCRAAWKQISVDGTSAAWRLMLPLSLSYDHRVIDGAAAARFNRHFAQ
                     LLAEMRRVLL"
     gene            complement(2046..>3254)
                     /gene="aceE"
                     /locus_tag="VFO53_03155"
     CDS             complement(2046..>3254)
                     /gene="aceE"
                     /locus_tag="VFO53_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633091.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HET8875303.1"
                     /translation="ATGEREISTTMAFVQMLGMLIRDKTLGRHVVPIVPDESRTFGME
                     GMFRQLGIYSAVGQLYKPQDADQLMYYREDRKGQVLQEGINEAGAMCSWIAAATSYST
                     NDMPMLPFYIYYSMFGFQRFGDFAWAAGDMRSRGFLLGGTAGRTTLNGEGLQHEDGHS
                     HVLASTIPNCVAYDPTFAYEVVVIIREGIRRMVEAQEDVYYYVTLMNENYAHPAMPAG
                     AEAGIIRGLYLLQAGAEATGPRVQLMGSGTILREVIAGAELLRNDFGIDADIWSAPSF
                     TELRRDGMSVERFNRLHPAQPPRRSYVETCLDGHAGPVVAATDYMRAYADQIRALVPR
                     RYVVLGTDGFGRSDYRVKLRRFFEVDRHHVAVAALKALADDGQIAVSVVADAIARYGL
                     DPEHPDPWTV"
BASE COUNT          493 a         1123 c         1095 g          543 t
ORIGIN      
        1 gcaggcgggg cgtagctgat gccgtgcgcg gccatcgtct tcacctcgtc catcacgaac
       61 gccgtgtgca gcaaagcctt cgacgggatg cagccgacgt tgaggcaaac gccgccgagc
      121 gtcgcgtagc gctcgacgag cacggtcctc atgccgagat cggcggcgcg aaacgcggcc
      181 gagtagccgc ccggacccga cccgagcacc agcatgtcgc attcgacgtc ggcacggccg
      241 gtgaaggtcg ccgggcccgc ggccggttgc ggtgtttgcg ccgcgggttt ggacgcttgc
      301 gctgccggcg cggcgggttt ggccggtgcg gcggtcgcgg tcggtggcgc gggggcggca
      361 gatgcacgct tcgcttccgc cggctggccg gcgcccggct cgccctcgag cgtcaggacg
      421 acgctgccct gcgacacctt gtcgcccacc ttcaccgcga cggccttcac cgtccccgca
      481 gcgggcgacg gcacgtccat cgtcgccttg tccgattcga gcgtgacgag cgcgtcttcc
      541 ggctcgacgg tgtcgccggg cttcacgagc acgtcgatga cgggcacgtc cttgaaatcg
      601 ccgatgtccg ggaccttcac ttcgacgctg ggcatgcggt tttcctgttg ttgtcgaaac
      661 ttcgattcag agcagcacgc gccgcatctc ggcgagcaac tgcgcgaaat ggcgattgaa
      721 ccgggcggcc gccgcgccgt cgatcacacg gtgatcgtac gacagcgaca gcggcagcat
      781 cagccgccac gcggcgctcg tcccgtcgac cgatatctgc ttccatgcag cgcgacagac
      841 gccgacgatc gcgacctcgg gcgcgttgat gatcggcgtg aaatacgtgc cgccgatgcc
      901 gccgaggctc gagatcgtga acgtgccgcc ctgcatgcga tcggcgccga gcttgccgtt
      961 gcgcgcgagc gtggcgagct cgcccgcttc cttcgcgatg tcgacgatgc ccttgcgatc
     1021 ggcgtcgcgt atcaccggca cgacgagacc gtccggcgta tcggcggcga agccgatatg
     1081 gtagtagcgc ttcaggatca gctcgtcgcc gtcgagcgat gcgttgaact cgggaaacgc
     1141 cgcgagcgtc gcgcacgcgg ccttgatcag gaacgcgagc atcgacacgc gcacgccgct
     1201 cttcgcgatc tcgccgttga gctcgatccg aaacgcctcg agctccgtga cgtcggcgtc
     1261 gtcgtgattg gtgacgtgcg ggatcgtcac ccagttccgc gcgaggttcg cgcccgagag
     1321 gcggcgaatg cgcgaaagcg gcttcgcttc gatcgggccg aacttcgcga aatcgacctt
     1381 cggccacggc agcacgtcga cgctcgtgcg cgcggcatgc acggattggt ccgaccgcgc
     1441 atcgcgcggc ggcaccgcgg cgtggacgac ccctttcgcg aaggcctcga cgtcctcgcg
     1501 cacgatcctg ccgttgcgcc cgcttccgct cacgtggacc aggtcgacgt cgagctcgcg
     1561 tgcgcgcctg cgcacggcgg gactggcgta tggcaaggcg atcggcgccg catcgctcgc
     1621 cggcgccgcg gtcgatcgcg tatgccgcgc ttctggcgtt tttggaggcg cagcgaattc
     1681 gggacgctgc gcctgcggtg ccgcgggcga tgccggcgcc tgcattgcat ccacttcggt
     1741 ggtcagcgtg gcgatcgtcg tgccttcgca gaccctgtcg ccgaccgcga cgcgaagctc
     1801 gcgaacggtg ccgccgagcg gcgacggcac gtccatcgtc gccttgtccg attcgagcgt
     1861 cacgagcgaa tcctcggcct cgacgacgtc gcccggcttc accatgatct caatgacagg
     1921 cacgtcgcga aagccgccga tgtcgggcac gcgcacctcg gcgatgccgg ccggcgactg
     1981 cgcgctggca ggcggtggcg acaccgacgc cttccccgcc cgcggcgctg ccggttcccg
     2041 cgtggtcata cggtccacgg atcgggatgc tcgggatcga gtccgtagcg cgcgatggca
     2101 tcggcaacga cactaaccgc gatctgtccg tcgtccgcaa gcgccttcaa cgccgcaacc
     2161 gcgacgtgat gacgatcgac ctcgaagaag cgccgcagct tgacccggta atcgctgcgg
     2221 ccgaatccgt cggtgccgag cacgacgtag cggcgtggca ccagggctcg aatctggtcg
     2281 gcgtacgcgc gcatgtaatc ggtggccgcg acgacgggac ccgcgtgccc gtcgaggcac
     2341 gtttcgacgt agctcctgcg cggcggctgc gccggatgca gccggttgaa acgctcgacg
     2401 ctcatgccgt cgcgccgcaa ttcggtgaag ctcggtgcgc tccagatgtc ggcgtcgatg
     2461 ccgaaatcgt tgcgcagcag ctcggcgccg gcaatcacct cgcgcaggat cgtccccgat
     2521 cccatgagct ggacgcgcgg gcccgtcgct tcggcaccgg cctgcaacag gtaaagcccg
     2581 cgaatgattc ccgcttccgc gccggccggc atcgcgggat gcgcatagtt ctcgttcatc
     2641 agcgtgacgt agtagtacac gtcttcctgc gcctccacca tgcgccggat gccttcgcgg
     2701 atgatgacga cgacctcgta ggcgaacgtc gggtcgtagg cgacgcaatt gggaatcgtc
     2761 gacgcgagga cgtggctgtg gccgtcctcg tgttgcagcc cctcgccgtt cagcgtcgtg
     2821 cggcccgcgg tgccgccgag caggaacccg cgcgagcgca tgtcgccggc cgcccacgcg
     2881 aaatcgccga agcgctggaa gccgaacatc gagtagtaga tgtagaacgg cagcatcggc
     2941 atgtcgttgg tgctgtacga cgtcgccgcg gcgatccacg agcacatcgc accggcctcg
     3001 ttgatgcctt cctgcagcac ctgccccttg cgatcctcgc ggtagtacat gagctgatcg
     3061 gcatcctgcg gcttgtagag ctggccgacc gccgaataga tgccgagctg gcggaacatc
     3121 ccttccatgc cgaacgtgcg cgactcgtcg ggcacgatcg gcacgacgtg cctgccgagc
     3181 gtcttgtcgc ggatcagcat gccgagcatc tgcacgaacg ccatcgtcgt cgagatttcg
     3241 cgctcgccgg tcgc
//
LOCUS       DASSVX010000069        18299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1402371, whole genome shotgun sequence.
ACCESSION   DASSVX010000069 DASSVX010000000
VERSION     DASSVX010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 18299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18299
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402371"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFO53_03160"
     CDS             complement(<1..516)
                     /locus_tag="VFO53_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005374157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8875304.1"
                     /translation="MHEPEAMRPVHAVAESRRARLPWRRANEVVVITGASAGVGRATA
                     RAFARRGARVGLIARDADALQATCRDVESLGGTAVPCVADVARAAEVESAAAMIEERL
                     GPIDVWINNAMVTIVSPVAELAADEVRRVTEVTYLGTVYGTLAALRRMRSRNRGAIVQ
                     VGSALAYRAIPL"
     gene            complement(503..952)
                     /locus_tag="VFO53_03165"
     CDS             complement(503..952)
                     /locus_tag="VFO53_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HET8875305.1"
                     /translation="MRAKPIDSGTGRTWAVIFDTGDEPMSALLAFATQQRLTAARFTA
                     IGAFRDATLGYFDVASKRYEKIPVREQVEVLSLIGDVALEDGKPKVHAHTVLGKRDGT
                     AHGGHLLEAHVRPTLEVMLTESPVALRRVFDPGVGLALIEIDRNARA"
     gene            complement(968..3931)
                     /locus_tag="VFO53_03170"
     CDS             complement(968..3931)
                     /locus_tag="VFO53_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HET8875306.1"
                     /translation="MRSEPDAHAATHDYRALERELATTVDAEVRFDIGSRALYATDGS
                     NYRQVPIGVVVPRTVDAVVATVAACRRHGAPVLPRGGGTSLCGQCCNVAIVIDFSKYL
                     NRIVELDASRKTAYVEPGCVLDTLRDAAEKHHLTFAPDPSTHSHNTLGGMIGNDSCGV
                     HSVMGGKTVDNVIELDVLTYEGVRFTARKLDDDERQRLLADDGRVAEIHRRLYALRDR
                     YADEIRARFPDIPRCVSGYGLTHLLPENGFDVARALVGTEGTCVVVLGAKLRLVDSPP
                     GRSLLVLGYPDVFGAADHVTDIMKHGPIGLEGIDDKLVADMKAVGLHPGDVELLPKGG
                     GWLLVEFGGRDRQESHAKARACMDAIGRTAGAPAMKLFDDPAQEKMIWTVREAGLGAT
                     AHVPNKTITWEGWEDAAVPPARLGDYLREFRQLLDRYDYAGDLYGHFGQGCVHTRIDF
                     DLETRAGIEKFHGFLDDASSLVVRYGGSLSGEHGDGQSKAEFLPKMFGAALVRAFGEF
                     KAIFDPRGKMNPGKVVDPYLPTQNLRLGDSFAPKQAVTFFKYPKDAGVFSRAALRCVG
                     VGNCRQHHHQTMCPSYRVTMEEKHSTRGRARLLFEMLRGDALTGGFRDENVKESLDLC
                     LACKGCKGDCPVHVDMATYKAEFLAHYYAGRMRPRSAYAFGLIDRWARIAAHAPGLVN
                     ALTQTAWSAVIAKRLAGVAPSRRLPRFAGETFQRRFARRPRPASADAGEVMLWPDTFN
                     NHFHPDTALAATDVLESAGFTVNVPAFAVCCGRPLYDSGMLREAQQYLRRILAQFQTE
                     IDAGMPFVVLEPACATVFRDEMPNLFPGDEVAKRLAAQTFLLAEFLTQRAPHFAPPPL
                     ARRAIVHGHCHQKAIMGMGADEAVLRKLGVRYDLLDSGCCGMAGSFGFERAKAAISVS
                     IGELVLLPAVRAAAADCLVIADGYSCREQIAQCTGRQAIHLADVLRMALAGAGVVAES
                     VAPADRDVASQTVPH"
     gene            complement(3940..5031)
                     /locus_tag="VFO53_03175"
     CDS             complement(3940..5031)
                     /locus_tag="VFO53_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET8875307.1"
                     /translation="MKRGVPIDRIEVSAYTIPTDAPESDGTLEWHQTTLVLVQARAGA
                     TAGIGYSYANRATAALIHDTLTSVVEGRDALDVAGAWDAMVASIRNLGRPGISSMAIA
                     AVDNALWDLKARLFDVPLVHLLGAARDAIPVYGSGGFTSYTLARLAEQLGGWAHSGIR
                     MVKMKIGREPAADVGRVRAARQAIGDDTSLFVDANGAYSRKEALAFADAFVQQRVTWF
                     EEPVPSDDLEGLRLVRDRAPASIDITAGEYGYDLVYFRRMCEAGAVDVLQADATRCAG
                     VTGFMRAATVAKAFHLPLSSHCAPSIHVHPCCAVNARHLEYFHDHVRIEHMLFDGVLD
                     PVDGMLRPASDRAGFGLAFKAADAKRYAV"
     gene            complement(5054..6820)
                     /locus_tag="VFO53_03180"
     CDS             complement(5054..6820)
                     /locus_tag="VFO53_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HET8875308.1"
                     /translation="MSNTVSDFLLQRLAAWGIKRIYGFPGDGINGILGALNRAGDEFE
                     FIQVRHEEMAAFMACGHAKFTGEVGVCLATSGPGAIHLLNGLYDAKLDHQPVVAIVGQ
                     SARAALGGSYQQEVDLTSLFKDVASEYVEMIVAPEQVRHVIDRALRIARAQRAPTCII
                     IPNDVQELPAEAPPRMHNTIHSGIGYAIARPIPHEHELQRAADALNAGGKVAMLVGAG
                     ALNATAEVIDVADVLGAGIAKALLGKAAVPDRYPFVTGSIGLLGTKPSYRMMMDCDTL
                     LMVGSNFPYSEFLPREGQARGVQIDIDARMLSIRYPMEVNLVGDSATTLRLLRPLLKR
                     KQDRAWRERIEKEMTDWWKTLEDRAMEDAHPINPQRVFWELSPRLPDRVIITCDSGSS
                     ANWYARDLRIREGMMASLSGGLATMGPGVPYAIAAKFAHPDRPVIALVGDGAFQMNGM
                     NEVLTIGKYWRRWSDPRLVIMVLNNRDLNQVTWEQRVMDGDPKYMASQDIPDYPYARY
                     ANDAGLHGIRVDRPEDVGRAWDEALASDRPVVLDIYTDPDVPPLPPHVTFKQARRFAE
                     SLVKGDPNTAGVIRASVKELFA"
     gene            complement(6942..7910)
                     /gene="dusA"
                     /locus_tag="VFO53_03185"
     CDS             complement(6942..7910)
                     /gene="dusA"
                     /locus_tag="VFO53_03185"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:NF008774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HET8875309.1"
                     /translation="MNDSTHRLCCAPMMDWTDRHCRYFFRLLTKRARLYTEMITAPAL
                     MHGDVPRHLDFDPAEHPVALQLGGSDPAQLAHAARLGERWGYDEINLNCGCPSQRVQT
                     GSFGACLMAEPALVAECVRAMRDAVQVPVTVKHRIGLDASEDYAFVRDFVGTVAVAGC
                     DVFIVHARNAVLKGLSPKQNREVPPLRYEFVHRLKRDFPRLAFVLNGGVHDFATIERE
                     LAHVDGVMLGRAAYHDPWLLAGVDARLYGDSPAVRGRAEVVEALIPYAERCLAQGTSL
                     RAIVRHILGLYHGVRGGRRFRQMLSDAARLHDADTSLLADALRVTG"
     gene            complement(7993..9447)
                     /gene="xylB"
                     /locus_tag="VFO53_03190"
     CDS             complement(7993..9447)
                     /gene="xylB"
                     /locus_tag="VFO53_03190"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HET8875310.1"
                     /translation="MFLGIDLGTSSLKAVLVDDAERIVGSASAPLTVANPHRGWSEQD
                     PRDWWTALLAAVDGLQRDHGAALAAVRGIGLCGQMHGAVVLDAADRVLRPCILWNDTR
                     SERECLALMEAEPALREITGNVAMPGFTAPKLLWIRTNEPRVFAATRRILLPKAWLRL
                     GLTGEAIEEMSDASGTLWLDVGARCWSSRMLAATGLDDRVMPSLVEGNATAGRLRVDL
                     AARWGMDVAPVVAGGAGDNAAGAIGVGAVRAGAAFVSLGTSGVIWTTTDRFRPYTEKA
                     VHAFCHALPGLWHQMGVTLSAAASLGWWAGVTGRAPAELIAEAGATTPRAADPIFLPY
                     LSGERTPHNDGSLRGTFVGLSATTDRAAMTRAIMEGVAFSLRDCLDALCASGTVIPEA
                     DVIGGGSRSGSWAAVLACALGIALHRVEEGNSGAAFGAARLARLAVTHEDPAAVCRPP
                     RRIGTAHPDQSTRAQYEERLARYRSCRPLSSLHV"
     gene            complement(9475..10953)
                     /locus_tag="VFO53_03195"
     CDS             complement(9475..10953)
                     /locus_tag="VFO53_03195"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HET8875311.1"
                     /translation="MTPAPLSRATLPLLSVAVSVPRYDPTKVRAGIVHLGLGGFHRAH
                     MARYMHDLMQLEPAALSWGILGAGLMPADRRMVQSLAPQDGLYTLVERSRSGEEVAVI
                     GSLAGVIDASESTEALLQAIDDRAIRIVSLTVTENGYCLNPATKRLDPEHPLVRADLA
                     HPQRPRSAIGVVVEALRRRHASGAPPPTVLSCDNIQHNGDVLRDATLALAALRDETLA
                     EWIRAEARFPNTMVDRITPVTAEADVSALASRHGIADRWPVVCETFRQWVIEDRFPQG
                     RPAWEDVGAQFVDDVAPYELIKLRLLNGSHLAVAGLGQLAGYETIDQSMADRRIAAVM
                     TTLMERETGPTLKPVPGIDLADYKRTLVERFANPAIRDTVQRVNTDAPVNVIIDPIRD
                     RLAAGASVEFLALALAAWMRRVRGVDGSGRPIRVVHPLADLLKAKAGEGGSNPAALLS
                     ITSLFGDLGADQRLVEPLAAWLGRFDREGIEATLHRAFQRAR"
     gene            complement(10987..11412)
                     /locus_tag="VFO53_03200"
     CDS             complement(10987..11412)
                     /locus_tag="VFO53_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004017388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET8875312.1"
                     /translation="MTLFVDTSVWSLAFRRDQVSAAPQVRVLGDALEAGETIVFTGLV
                     LQELLQGFSGPKARKDIIDRFAALPLLAPDRLDHIDAAELRNRCRRAGLQIGTIDAVI
                     AQLCIRHSLVLLTTDNDFVLAARHCPLRVWGNENRSNHR"
     gene            complement(11409..11609)
                     /locus_tag="VFO53_03205"
     CDS             complement(11409..11609)
                     /locus_tag="VFO53_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HET8875313.1"
                     /translation="MATNLDLDPDLIERAVKVSGEKTKKAAVTRALEEFITRRSQKRL
                     AELMGKLEWNESFDYKAERSRR"
     gene            11835..16145
                     /locus_tag="VFO53_03210"
     CDS             11835..16145
                     /locus_tag="VFO53_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8875314.1"
                     /translation="MDDNRPPASSARAACDDVASAAAGVAFLERGGEMGRRMRAFDWA
                     ATALGPPQSWPQSLRTVVRILLDSRYAMWMLWGPDLTFFCNDAYLPTVGIRRDWVLGA
                     RSDKVWAEIWPDIGPRIAHVLETGEATWDQGLMLLLERSGYPEETYHTFSYSPVYDDA
                     GSIAGMLCVVTEDTERRISERRIALLGAIGADLAATNSEADVFAGAARHLGGLPDLPC
                     AVVFIQRAGTLQCALRAGFDDANGGAEDRSGDAHGVAGERIDDAPWPLADVLASGVEQ
                     TIELDARFGDLPRGPWPDAPRTALLLPIQQQGHAQPAGVFVACLNRYRPLDDAYRRFL
                     RLLAAQIASAVANARSYAGERRRAEALAEIDRAKTAFFSNVSHEFRTPLTLMMGPLED
                     ALRDDALASDVRDSLAVAHRNSVRLLKLVNSLLDFSRIEAGRLRARYVPTDIATLTAE
                     LASVFRSAIERAGVALHVDCVPVADVHVDRDMWEKIVFNLLSNALKHTHQGDIRVRVV
                     PAGDMFELSICDTGIGIPADALPQVFDRFYRVPNARARTHEGSGIGLALVQEFVKLHG
                     GTVRVASEVDVGTTFTVALPRNRAHLPDDATDAAAEPAGASGYAEAFVDEALSWQPDA
                     SPRTRPPRAPADADTVLLVDDNADMREYVRTLLSSRFHVRTAADGLEALREIERARPD
                     LVLTDVMMPRLDGFGLLKALRDNAATRELPVIVLSARAGEEARIEGIDAGADDYLVKP
                     FSARELHARVGNTLAMARARREYVLATRAGEARRLFLLDFADALHAAQSPEEVVAAAL
                     SGIGGELATACAGYVEVERDGTAFRVVAEWRADGAKGRAGERFPIDHFGEEEARRAGL
                     PIVIADTRTHRRAEAWLAEGTGAVVTVPSVTAGRSPAALWVSMPGPRAWHGDEIALMR
                     EAAERVWAELGRARAESALRESEERFRAMADSSPLLVWVVDPHGRVVFANRACQEFFG
                     RAPARLGPDGWRAYLHPDDADGYTSEVLAALAARRPFSVMGRVRRSDGVWRWVQSIGA
                     PRFSEAGEFLGAVGSSPDVTELIEASDALRDADRRKDEFLATLAHELRNPLAPIRQAA
                     RLSRSPEASDAQRRWSQEVIERQVKHMALLLDDLLDVSRITRGKLELRRARVDLASVV
                     AMALETAHPLIDDRRQTVIHDLPAAPIILDADSMRLAQVLANLLTNAAKYSEPGGIIE
                     LAAVREVDEIRIDVKDGGIGIEPTLLPRVFEMFSQMHGSLDRAEGGLGIGLALVKGLV
                     ELHGGRVEAHSAGLGHGAQFSVRLPLPPPGDAPEAPAGGGGHAEAPDMRHARILVADD
                     NRDAASSLATLLMLDGHDVRVANDGARALVEAESFRPHIALLDIGMPMHNGYDVARAI
                     RAAPWGRSMVLVAVTGWGQSEDKRRAKEAGFDHHFTKPLDLDVLGAFVTDALARQPAR
                     "
     gene            16117..17757
                     /locus_tag="VFO53_03215"
     CDS             16117..17757
                     /locus_tag="VFO53_03215"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8875315.1"
                     /translation="MRLPGSLRAEAPPSGGAAANPRTRGERWPSSAVAAAYIALYVAL
                     DWVSFIDPVAAFAITPWNPPPGLSVMFLLRRGLRQAPWLFVAALAAEFLVRGAPAPLP
                     LLIAASLLLAASYTAVTALLTRVLHVDPDLASLRDASMLVVTAAVACGALALAFVALF
                     AGSGLLPAGAFWRSAMQFWIGDLIGIVVTAPVLLVVTRKRNRPALRISVEGFAQAAAI
                     GVALWMVFGAGLPDATTLFYVLFLPVIWIAMRHGIEGAVFGTLATQAGLIVAIVVTGQ
                     GRDAVLEFQFLMLAIATTALLLGAAVSERRTIERTLREKQATLDRSLRLAAASELASS
                     LAHELNQPLSAIIAYLRACELMLARPGDDTAELRATMNKVIAEASRAGSVVRRLRDFF
                     RSGSARIETVAPATLIAEACAAVIERARRNGVSVEQSARNLPGVRVDRVQIEAVLANL
                     LGNAIDALQQRPGEREVRIDASPDTPGFVRIAVTDNGPGVDPEITQSLFDAFVTTKAS
                     GMGLGLAISRSIVDAHGGRLWHEARNPGSRFCFTVPAA"
     gene            17840..>18299
                     /locus_tag="VFO53_03220"
     CDS             17840..>18299
                     /locus_tag="VFO53_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8875316.1"
                     /translation="MIAQRPLVAIVDDDEAVRDALGLLLRLHAFDVRAFASGEQFLAW
                     AGTQPVDCVLLDLRMSGLPGLDVQAALASRGLDYPIIVVTAHGDVATTRAALKAGAFD
                     FIEKPFDDELLLRTVRAASEASTRQRARRARRALCAEHRPVDAARARSARP"
BASE COUNT         2839 a         6305 c         6241 g         2914 t
ORIGIN      
        1 cagcggaatc gcccggtagg caagcgccga gcccacctgc acgatcgcgc cgcgattgcg
       61 cgagcgcatg cgccgcagcg ccgcgagcgt tccgtaaacc gtcccgaggt aggtgacctc
      121 ggtgacgcgc cgcacttcgt ccgccgcgag ctccgcgacc ggcgagacga tcgtcaccat
      181 cgcgttgttg atccacacgt cgatcggccc cagccgctcc tcgatcatcg ccgcggcgct
      241 ttccacttcg gccgcgcgcg cgacatcggc gacgcacggc acggccgtgc caccgaggga
      301 ttcgacgtcc cggcacgtcg cctgcagcgc gtcggcgtcg cgggcgatga gaccgacgcg
      361 cgcgccgcgt cgcgcaaacg cacgcgcggt tgcgcgtccg acgccggcgc tggcgccggt
      421 gatgacgacc acttcgttcg cccgccgcca cggcagcctt gcacgccgcg attcggcaac
      481 cgcatgcacc ggccgcatcg cctcaggctc gtgcattgcg gtcgatctcg atcagggcga
      541 gtccgacgcc ggggtcgaac acccggcgaa gcgccacggg cgattcggtc agcatcacct
      601 cgagcgtcgg acgcacgtgc gcttcgagca gatgtccgcc atgcgcggtg ccgtcgcgct
      661 tgccgagcac cgtgtgcgcg tgaaccttcg gcttgccgtc ttccagcgcc acgtcgccga
      721 tcagcgaaag cacctcgacc tgctcacgca cgggaatctt ctcgtaccgc ttcgatgcca
      781 cgtcgaaata gccgagtgtt gcatcgcgaa acgcaccgat cgcggtgaat cgcgccgcgg
      841 tgagccgctg ctgcgttgca aacgcgagca gcgcgctcat cggttcgtcg ccggtgtcga
      901 agatcactgc ccaggtccgg ccggtgccgg agtcgatcgg cttcgcgcgc atggtcgggc
      961 cccttcgtca gtgcggcacc gtttggcttg ccacgtcgcg gtccgcgggt gcaacggatt
     1021 cggcgacgac gcccgccccg gcgagcgcca ttcgcagcac gtcggcgaga tgaatggcct
     1081 gccggccggt gcactgcgca atctgctcgc ggcagctata gccgtcggcg atcacgaggc
     1141 agtcggcggc tgcggcacgt acggcgggca gcagcacgag ctcgccgatc gaaaccgaga
     1201 tggccgcttt cgcgcgctcg aagccgaacg agccggccat gccgcagcag cccgagtcga
     1261 gcaggtcgta gcgcacgccc agcttgcgca gcaccgcttc gtcggcgccc atgcccatga
     1321 tcgccttctg atgacaatgc ccgtgcacga tcgcgcggcg ggcgagcggc ggcggcgcga
     1381 agtgcggcgc gcgctgcgtg aggaattcgg cgagcaggaa cgtctgtgcc gcgagccgct
     1441 tcgcgacttc gtcgccggga aacagattcg gcatctcgtc gcgaaacacc gtcgcgcacg
     1501 cgggctcgag cacgacgaac ggcatgccgg cgtcgatttc ggtttggaac tgcgcgagga
     1561 tgcggcgcag gtactgctgc gcctcgcgca acatgcccga gtcatacagc ggccgcccgc
     1621 agcagacggc gaacgccggc acgttgaccg tgaagccggc ggattcgagc acgtcggtgg
     1681 cggcgagcgc cgtgtccgga tggaagtgat tgttgaacgt gtcgggccac agcatgacct
     1741 cgcccgcgtc cgcgctcgcc ggtcgcggac ggcgcgcgaa gcggcgctgg aacgtttctc
     1801 cggcaaaacg cggaaggcgt cgcgacggcg cgacgccggc gagccgtttc gcaatgacgg
     1861 ccgaccacgc cgtctgcgtc agtgcattca cgagaccggg tgcgtgcgcc gcgatacgcg
     1921 cccagcgatc gatgagtccg aacgcatagg ccgagcgcgg acgcatccgt ccggcgtagt
     1981 aatgcgcgag gaactcggcc ttgtacgtcg ccatgtcgac gtgcaccggg cagtcgccct
     2041 tgcagccctt gcacgcgagg cacaggtcga gcgattcctt gacgttttcg tcgcgaaagc
     2101 cgccggtgag cgcatcgccg cgcagcatct cgaacagcag ccgcgcgcga ccgcgggtcg
     2161 aatgcttctc ttccatggtc acgcggtagc tcgggcacat cgtctggtga tgatgctggc
     2221 ggcagttgcc gacgccgacg cagcgcagcg ccgcgcggct gaacacgccc gcgtccttcg
     2281 gatacttgaa gaacgtcacc gcctgcttcg gcgcgaagct gtcgccgagc cgcaggttct
     2341 gcgtcggcaa ataggggtcc accaccttgc ccggattcat ctttccgcgc ggatcgaaga
     2401 tcgccttgaa ctcgccgaac gcgcgcacga gtgcggcgcc gaacatcttc ggcaggaact
     2461 cggccttcga ctggccgtcg ccgtgctcgc cggacagcga gccgccgtag cgcacgacga
     2521 ggctcgacgc atcgtcgaga aagccgtgga atttctcgat gcccgcgcgc gtctcgaggt
     2581 cgaagtcgat gcgcgtgtgc acgcagccct gcccgaaatg accgtagagg tcgcccgcgt
     2641 aatcgtagcg gtcgagaagc tggcgaaact cgcgcaggta atcgccgaga cgcgccggcg
     2701 gaacggccgc gtcctcccag ccctcccagg tgatcgtctt gttcggcacg tgcgcggtgg
     2761 caccgagacc cgcttcgcgc accgtccaga tcattttctc ctgcgccggg tcgtcgaaga
     2821 gcttcatcgc cggtgcgccg gccgtcctgc cgatcgcgtc catgcacgca cgcgccttcg
     2881 catgcgactc ctgccgatcg cggccgccga attcgaccag cagccagcca ccgcccttcg
     2941 gcagcagctc gacgtcgccg ggatgcaggc cgaccgcctt catgtcggcc acgagcttgt
     3001 cgtcgatgcc ctcgagaccg atcgggccgt gcttcatgat gtcggtgaca tggtcggcgg
     3061 cgccgaacac gtcgggataa ccgagcacga ggagcgagcg tcccggcgga ctgtcgacaa
     3121 gccgcagctt cgcgccgagc acgacgacgc acgtgccttc ggtgccgacc agcgcgcgcg
     3181 ccacgtcgaa accgttttcc ggcagcaggt gcgtcaggcc gtagcctgac acgcagcgcg
     3241 gaatgtcggg aaagcgcgcg cggatttcat cggcgtagcg gtcgcgcagc gcataaaggc
     3301 gccgatggat ctcggcgacc ctgccgtcgt cggcgagcag gcgctgccgc tcgtcgtcgt
     3361 cgagcttccg cgccgtgaag cgcacgcctt cgtacgtcag cacgtcgagc tcgatgacgt
     3421 tgtcgacggt cttgccaccc atcaccgaat gcacgccgca cgagtcgttg ccgatcatgc
     3481 cgccgagcgt gttgtggctg tgcgtcgacg gatcgggcgc gaacgtcagg tgatgcttct
     3541 cggccgcatc gcgcagcgtg tcgagcacgc aacccggctc gacgtacgcg gtcttgcgcg
     3601 atgcgtcgag ctcgacgatg cggttcaggt atttggagaa atcgatgacg atggcgacgt
     3661 tgcagcattg cccgcagagg ctggtgccgc cgccacgcgg gagcaccggc gcgccgtggc
     3721 gccggcatgc ggcaacggtg gcaaccaccg cgtccaccgt gcgcggcacg acgacgccga
     3781 tcggcacttg ccgatagttg gagccgtcgg ttgcgtacag cgcgcggctg ccgatgtcga
     3841 agcgcacttc ggcgtcgacg gtggttgcga gctcgcgctc gagcgcacgg tagtcgtgcg
     3901 tcgccgcgtg cgcgtccggc tcgcttcgca tcgcgtcgtt cataccgcat accttttcgc
     3961 gtcggccgcc ttgaacgcga ggccgaagcc cgcgcggtcg gatgccgggc gcagcatgcc
     4021 gtcgaccgga tcgagcacgc cgtcgaacag catgtgctcg atgcgcacgt ggtcgtgaaa
     4081 gtattcgagg tgccgcgcgt tcaccgcgca gcagggatgc acgtggatcg atggcgcaca
     4141 gtggctcgac agcggcaggt ggaacgcctt cgccaccgtt gcggcacgca tgaaaccggt
     4201 gacacccgca cagcgggtag cgtccgcctg cagcacgtcg accgcacccg cttcgcacat
     4261 gcggcgaaaa tagacgaggt cgtagccgta ttcgccggcc gtgatgtcga tcgacgcagg
     4321 cgcgcggtcg cggacgagcc gcagtccctc gagatcgtcg ctcggcaccg gctcctcgaa
     4381 ccacgtcacg cgctgctgca cgaacgcgtc ggcgaatgcc agcgcctcct tgcggctgta
     4441 cgcaccgttc gcatcgacga aaagcgacgt gtcgtcaccg atggcctgcc gcgccgcgcg
     4501 cacgcggccg acgtcggccg cgggctcgcg cccgatcttc atcttgacca tgcgtatgcc
     4561 cgaatgcgcc cagccgccaa gctgttcggc gagccgcgcg agcgtgtacg acgtgaagcc
     4621 gccgctgccg taaacgggaa tcgcgtcgcg cgcggcgccc agcagatgca cgagcggcac
     4681 gtcgaaaagc cgcgccttca ggtcccacag cgcattgtcg acggcggcga tcgccatcga
     4741 cgaaatgccg ggacggccga gattgcggat cgacgccacc atcgcgtccc atgcacccgc
     4801 gacgtcgagc gcatcgcgcc cttcgacgac gctagtcagc gtgtcgtgga tcagcgcggc
     4861 cgtggcgcgg ttcgcgtagc tgtagccgat gcccgccgtt gctccggccc tcgcctgcac
     4921 gagcacgagc gtcgtctggt gccactcgag cgtcccgtcg gactcgggcg cgtcggtcgg
     4981 tatcgtgtac gccgatacct cgatgcgatc gatcggcacg ccgcgcttca tttcgactca
     5041 cccgtcgcgc gcgtcaggca aaaagctcct tcaccgacgc gcggatcacg ccggccgtgt
     5101 tcggatcgcc cttcacgagc gattccgcga atcgccgcgc ctgcttgaac gtcacgtgcg
     5161 gcggcagcgg cggcacgtcg ggatcggtgt agatgtccag cacgaccggc cgatcgctcg
     5221 ccagcgcctc gtcccaggcg cgacccacgt cctcgggccg gtcgacacgg atgccgtgca
     5281 ggcccgcgtc gttcgcgtag cgcgcgtacg gataatcggg gatgtcctgc gacgccatgt
     5341 acttcggatc gccgtccatc acgcgctgct cccacgtcac ctggttgagg tcgcggttgt
     5401 tcagcaccat gatgacaaga cgcggatcgg accagcggcg ccagtacttg ccgatcgtca
     5461 gcacctcgtt catgccgttc atctggaacg cgccgtcgcc gacgagcgcg atcaccgggc
     5521 ggtcgggatg cgcgaactta gccgcgatcg cgtacggcac gcccggaccc atcgtcgcca
     5581 gcccgcccga cagcgacgcc atcatgcctt cgcggatcct gaggtcgcgt gcgtaccaat
     5641 tcgccgacga gcccgagtcg caggtgatga tcacccggtc gggtagccgc ggggacaatt
     5701 cccagaacac gcgctgcgga ttgatcgggt gcgcgtcttc catcgcgcga tcctcgagcg
     5761 tcttccacca gtcggtcatt tccttctcga tgcgctcgcg ccacgcgcga tcctgcttgc
     5821 gcttcagaag cggccgcagc agccgcagcg tcgtggcact gtcgccgacc aggttgactt
     5881 ccatcggata gcggatcgag agcatccgcg cgtcgatgtc gatctgcacg ccgcgcgcct
     5941 gcccctcgcg cggcaggaac tcggaatacg gaaagttcga gccgaccatc agcagcgtgt
     6001 cgcagtccat catcatccgg tacgagggct tcgtgccgag gagcccgatc gagccggtaa
     6061 cgaatggata cctgtcgggc accgcggcct tgccgagcag cgccttggcg atacccgcgc
     6121 cgagcacgtc ggccacgtcg atcacctcgg cggtggcgtt cagcgcgccg gcgccgacga
     6181 gcatggcgac cttcccgccc gcattcagcg catcggcggc gcgctgcagc tcgtgttcat
     6241 gcgggatcgg ccgcgcgatc gcgtacccga tgcccgaatg gatcgtgttg tgcatgcgcg
     6301 gcggcgcctc ggccggcaat tcctgcacgt cgttcggaat gatgatgcag gtcggcgcac
     6361 gctgggcacg cgcgatgcgc agcgcgcggt cgatcacgtg gcgcacctgc tcgggtgcca
     6421 cgatcatctc gacgtactcg ctcgcgacgt ccttgaacag cgacgtcagg tccacttcct
     6481 gctggtagct gccgccgagt gctgcgcgcg ccgattggcc gacgatggcg accaccggct
     6541 gatgatcgag cttcgcgtcg tacagtccgt tcagcagatg gatcgcgccc ggacccgatg
     6601 tggcgaggca gacgccgacc tcgccggtga acttcgcatg gccgcaggcc atgaacgccg
     6661 ccatttcctc gtgacggacc tggatgaatt cgaactcgtc gccggcgcgg ttgagcgccc
     6721 cgaggatgcc gttgatgccg tcgccgggga agccgtagat ccgcttgatg ccccatgcgg
     6781 cgagccgttg cagcaggaag tcgctgaccg tgttgctcat cgtcgttcgc tcctcgacag
     6841 gaatgcgggc gcgaacctgg tcgtaccctt tcatccggct tccggttgga tgcgacgctg
     6901 ccggtcgagt tcaacgcggc gcgagttgca agctttacaa atcagccggt gacgcgcagc
     6961 gcatcggcga gcagcgacgt gtcggcatcg tgcaggcgcg cggcgtcgga gagcatctgc
     7021 cgaaagcgtc gcccgccgcg aacgccgtgg tagaggccga gaatgtggcg cacgatcgcg
     7081 cgcagtgacg tgccttgcgc aagacatcgt tcggcgtacg ggatcagcgc ctcgacgacc
     7141 tcggcacgcc cgcgcaccgc cggcgaatcg ccgtagaggc gcgcatcgac gccggcgaga
     7201 agccacggat cgtggtaggc ggcgcgtccg agcatcacac catcgacgtg cgcaagctcg
     7261 cgctcgatcg tggcgaagtc gtgcacgccg ccattgagca cgaaggcaag ccgcgggaag
     7321 tcgcgcttca gccggtgcac gaattcgtag cgaagcggcg gcacctcgcg gttctgcttc
     7381 ggcgacaggc ccttcagcac cgcatttcgc gcatgcacga tgaacacgtc gcatcccgcg
     7441 accgcgacgg tgcccacgaa atcgcggacg aacgcgtaat cttcgctcgc gtcgagaccg
     7501 atgcggtgct tcaccgtcac cggcacctgc acggcgtcgc gcatggcgcg cacgcattcg
     7561 gcgacgagcg ccggctcggc catgaggcac gcgccgaagc tgcccgtctg cacgcgctgt
     7621 gacgggcagc cgcagttcaa gttgatctcg tcgtaacccc agcgctcgcc gagccgtgcc
     7681 gcgtgcgcga gttgcgcggg gtcgctgccg ccgagctgca acgcgaccgg atgctcggcg
     7741 ggatcgaagt cgagatggcg cggcacgtcg ccgtgcatca gcgccggcgc ggtgatcatc
     7801 tcggtgtaga gccgggcgcg cttcgtcagc agccggaaga agtaccggca atgccgatcg
     7861 gtccagtcca tcatcggcgc gcaacataac cgatgcgttg agtcgttcat ggtcttggat
     7921 gcgctcgatc ggttcgacag gggcgtagca agtcaccgca tcgcggccgg atctcgctcg
     7981 gcccgcgcgg ggtcagacgt gcagcgagga tagcgggcga cacgaccggt atcgcgccag
     8041 gcgttcctcg tattgcgctc gggtcgattg atccgggtgc gctgtcccga tgcgccgcgg
     8101 aggtcgacag acggcggcag ggtcctcgtg cgtcaccgcg agccgcgcaa gccttgcagc
     8161 gccgaatgcc gcgcccgagt ttccctcctc gacgcgatgc agcgcgattc caagcgcgca
     8221 cgcgagaacc gcggcccagg atccggagcg cgagccgccg ccgatgacat cggcctcggg
     8281 aatcacggtg cccgacgcgc aaagtgcatc caggcaatcg cgcaacgaaa atgccacgcc
     8341 ttccatgatc gcccttgtca tcgcggctcg atcggtcgtc gccgacagtc cgacgaatgt
     8401 gccgcgcagg ctgccgtcgt tatgcggcgt gcgctcgcct gaaaggtagg gcaggaagat
     8461 cggatcggca gcacgcggtg tggtggcgcc ggcctccgcg atcagctcgg ccggcgcgcg
     8521 gccggtcaca ccggcccacc agccaagcga ggccgccgcc gacagcgtca cgcccatctg
     8581 atgccagaga cctggcaggg catggcaaaa agcgtgaacg gccttttcgg tgtacggacg
     8641 gaagcgatcg gtcgttgtcc agatcacgcc ggacgtgccg agagaaacga aggctgcgcc
     8701 cgctcgcact gcgcctacgc caatcgcgcc ggcggcattg tcacctgccc cgcccgcgac
     8761 gactggcgcc acgtccattc cccatcgtgc ggcgaggtcc acgcgcagtc ggcccgcagt
     8821 ggcgttgcct tcgacgagcg agggcatgac tcgatcgtcc agcccggttg cagcgagcat
     8881 tctcgacgac cagcaacgcg cgccgacgtc gagccatagc gtgcccgagg cgtcggacat
     8941 ttcctcgatc gcctcgccgg tgaggccaag acgcaaccac gccttgggca gcaggatgcg
     9001 gcgcgtcgcc gcgaacactc gcggctcgtt cgtcctgatc caaaggagct tgggcgcggt
     9061 gaatcccggc atcgccacgt tgccggtgat ttcgcgaagc gccggttctg cctccatgag
     9121 cgcgaggcat tcacgctcgg aacgcgtgtc gttccacagg atgcacggtc ggagcacccg
     9181 gtcggcggca tcgagtacga cggcaccgtg catttgtccg caaaggccga taccgcgcac
     9241 ggcggcgagc gctgcaccgt ggtcgcgctg caggccgtcg acagccgcca gcagcgctgt
     9301 ccaccagtcg cgcggatcct gctcgctcca gccgcggtgg ggattggcga ccgtcagcgg
     9361 cgccgatgcg ctgccgacga tgcgctcggc gtcgtccacc agcacggctt tgaggctcga
     9421 ggtgccaagg tcgatcccca ggaacatgca gcgtctcccc gacgggccag cgcgctaccg
     9481 ggcgcgttgg aaggcccggt gcagcgttgc ttcgatgcct tcgcgatcga atcgcccgag
     9541 ccatgcggcg agcggctcga cgagcctctg atcggcaccg agatcaccga acagcgacgt
     9601 gatcgacagc aacgccgccg gattcgaacc gccctccccg gccttcgcct tcaggagatc
     9661 ggcgagcgga tgcaccacgc ggatcggccg gccactgccg tcgaccccgc gcaccctgcg
     9721 catccacgcg gcgagcgcga gcgcgaggaa ttcgacgctg gcgcccgcgg ccaaacggtc
     9781 gcgaatcgga tcgatgatca cgttgaccgg tgcatcggtg ttgacacgct gcacggtgtc
     9841 gcggatcgcg gggttggcga agcgctcgac gagcgtgcgc ttgtaatcgg ccaggtcgat
     9901 ccccggcacc ggcttcagcg tcggcccggt ctcgcgctcc atcaacgtcg tcatcacggc
     9961 ggcgatgcga cgatcggcca tcgactggtc gatcgtttcg tatccagcga gctggccgag
    10021 gcccgccacg gcaaggtggc tgccgttgag aagcctcagc tttatgagct cgtagggcgc
    10081 cacgtcgtcg acgaactggg cgcccacgtc ttcccaggcg ggtcggcctt gagggaatcg
    10141 atcctcgatc acccattgcc tgaacgtctc gcagaccacc ggccagcgat cggcgatacc
    10201 gtgccggctt gcaagcgcgc tcacgtcggc ttcggccgtg accggggtga tgcggtcgac
    10261 catggtgttg gggaagcgag cttcggcacg gatccattcc gcgagcgtct cgtcacgcag
    10321 cgcggcaagc gccagcgtgg cgtcgcgtaa tacgtcgccg ttgtgctgaa tgttgtcgca
    10381 gctcagcacc gtcggtggcg gtgcgcccga tgcgtgtcga cgacgcagtg cttcgacgac
    10441 gaccccgatg gcgctgcgcg gacgctgcgg atgcgcgagg tcggcgcgaa ccagcggatg
    10501 ctcgggatcg aggcgcttgg tcgccggatt caggcaatag ccattttcgg tgaccgtcag
    10561 gctgacgatg cggatcgcgc gatcgtcgat cgcctgcaac aacgcttcgg tcgactcgct
    10621 ggcgtcgatc acgccggcga gcgagccgat gacggccacc tcttcgccgc tgcgggagcg
    10681 ctcgaccagc gtgtagaggc cgtcctgtgg tgcgaggctc tgcaccatcc ttcgatcggc
    10741 gggcatcagg ccggcaccaa ggatgcccca ggacaacgcg gccggttcga gttgcatcaa
    10801 gtcgtgcatg tagcgcgcca tgtgcgcgcg gtggaatccg ccgaggccca gatgcacgat
    10861 accggcacgc accttcgtcg ggtcgtagcg cgggacgctg accgcaacgc tcagcagcgg
    10921 caaggtcgcg cgggacagcg gcgccggcgt cattgcagcc ggctccgtgc cgacgggcct
    10981 gcacattcaa cgatggttgg accgattttc attcccccaa acccgcagtg gacagtgccg
    11041 cgcggcgagg acgaaatcgt tgtcggtggt cagcagcacc agactgtgac gaatgcagag
    11101 ctgcgcaatt accgcatcga tcgtgccgat ctgcaagcca gcgcggcggc agcgattgcg
    11161 cagctccgca gcgtcgatgt gatccagccg atccggagcc aggagcggca gcgccgcaaa
    11221 acgatcgatg atgtcctttc gcgccttcgg gccggaaaat ccctgcagca attcctgcaa
    11281 gacgaggcct gtgaatacga ttgtttcgcc ggcctcgagc gcatcgccca acacgcgaac
    11341 ctgcggggcc gcagaaacct ggtcacgtcg aaacgcgagc gaccacacgc tcgtatcgac
    11401 gaacagggtc acctgcgcga tcgctcggct ttgtagtcga aggactcatt ccattcgagc
    11461 tttcccatca actcggccag gcgcttctgg ctgcggcggg tgatgaactc ctccagcgcg
    11521 cgggtaaccg ccgccttttt ggtcttttca ccgctcacct tcacggcgcg ttcgatgaga
    11581 tccgggtcca ggtcaagatt ggtcgccatg tgcatctcct tacacagcag attacacaaa
    11641 cggcacttgc ccggcaaggg accgttcgac gagggaccgt tcgacgagca accgatcgcg
    11701 ggtggattcc gcaatgcaga tcgctcgagc gcggcgatgt tgcgccccgc gccggccgtg
    11761 cccgacggca accggctaag atgtcggtcg cgcggcgctc gcaatggcgc ggcccgccgt
    11821 tcgccccccg ttcgatggac gacaaccgcc cgccagcctc gtctgcgcgc gctgcctgcg
    11881 acgacgtcgc gagcgcggcg gccggcgttg cctttctcga gcgcggcggc gaaatgggcc
    11941 ggcggatgcg cgccttcgac tgggcggcga ctgcgctcgg gccgccgcaa tcgtggccgc
    12001 agagcctcag gaccgtcgtg cgcatcctgc tcgattcgcg ctacgcgatg tggatgctgt
    12061 ggggtcccga tctcacgttc ttctgcaacg acgcgtatct gccgacggtc ggcatccggc
    12121 gcgactgggt gctcggtgca cgctccgaca aggtgtgggc cgaaatctgg ccggatatcg
    12181 ggccgcgcat cgcgcacgtg ctcgaaaccg gcgaggcgac gtgggaccag ggtctgatgc
    12241 tgctgctcga gcgcagcggc tacccggaag agacctacca cacgttttcg tacagtccgg
    12301 tttacgacga cgccggcagc atcgccggca tgctgtgcgt cgtgaccgag gacaccgagc
    12361 ggcggatcag cgagcgccgc atcgcgctgc tgggcgcgat cggggccgat ctcgcagcca
    12421 cgaattccga ggccgacgtg ttcgcgggtg cggcgcgcca cctcggcggc ctgcccgacc
    12481 ttccctgcgc cgtcgtcttc attcagcgcg ccggcacgct gcaatgcgcg ctccgcgcgg
    12541 gcttcgacga cgcgaatggc ggcgcggagg atcgatccgg ggacgcgcat ggcgtggcgg
    12601 gggagcggat cgacgatgcg ccgtggccgc tcgcggacgt gctcgcaagc ggtgtcgagc
    12661 aaacgatcga gctcgacgcg cgcttcggcg atctgccgcg cggtccgtgg cccgatgcgc
    12721 cgcgaacggc gttgctgctg ccgatccagc agcaagggca cgcgcaaccg gccggggtgt
    12781 tcgtcgcctg cctcaaccgc taccgcccgc tcgacgatgc ctatcggcgc tttctcaggc
    12841 tgctggccgc gcagatcgcg tcggccgtcg cgaacgcgcg aagctacgcc ggcgagcggc
    12901 gccgcgccga ggcgctcgcg gagatcgacc gcgcgaagac cgcgttcttc tcgaacgtga
    12961 gccacgaatt ccgcacgccg ctcacgctga tgatgggccc gctcgaggac gcgctgcgcg
    13021 acgacgcgct ggccagcgac gtgcgcgaca gtctcgcggt tgcgcatcgc aacagcgtgc
    13081 gcctgctgaa gctcgtcaat tcgctcctcg acttttcccg catcgaggcc gggcggctgc
    13141 gcgcgcgcta cgtaccgacc gacatcgcga cgctgaccgc cgagctcgcg agcgtcttcc
    13201 gctcggcgat cgagcgtgcg ggcgttgccc tgcacgtcga ctgcgtgccg gttgccgatg
    13261 tgcacgtcga ccgcgacatg tgggaaaaga tcgtgttcaa cctgttgtcg aacgcgctga
    13321 agcacacgca ccagggcgac atccgcgtgc gcgtggtacc cgccggcgac atgttcgagc
    13381 tgtcgatttg cgacacgggc atcggcattc ccgccgatgc gctgccgcag gtgttcgacc
    13441 gcttctaccg cgttccgaac gcgcgtgcac gcacgcacga gggctcgggc atcggccttg
    13501 cactcgtgca ggagttcgtc aagctgcacg gcggcacggt gcgcgtggcg agcgaagtcg
    13561 acgtcggcac gacattcacc gtcgcgctac cgcgcaatcg ggcgcatctg cccgatgacg
    13621 cgacggacgc ggcggccgag ccggcaggcg cgtccgggta cgcggaggcg ttcgtcgacg
    13681 aggcgctttc ctggcaaccc gatgcgtcgc cccgcacccg cccgccacgg gcgcccgccg
    13741 acgccgatac ggtgctgctc gtcgacgata acgccgacat gcgcgaatac gtgcgcacgc
    13801 tcctgtcgtc gcgcttccat gtgcgaaccg cggccgacgg cctcgaggcg ctgcgcgaga
    13861 tcgagcgcgc gcgccccgat ctcgtgctga ccgacgtcat gatgccgagg ctcgacggct
    13921 tcggtctgct gaaggcgctg cgcgacaacg cggccacgcg cgagcttccg gtgatcgtcc
    13981 tttcggcacg cgccggcgag gaggcgcgca tcgagggcat cgacgccggc gccgacgatt
    14041 atctcgtcaa gccgttctcg gcgcgcgagc tgcatgcgcg cgtgggcaac acgctcgcga
    14101 tggcgcgcgc gcgccgcgag tacgtgctgg cgacgcgcgc cggagaggca cgccggcttt
    14161 tcctgctcga cttcgcagac gcgctgcatg ccgcgcaaag ccccgaggaa gtcgttgcgg
    14221 cggcgctttc gggcatcggt ggcgagcttg cgaccgcctg cgccgggtat gtcgaagtcg
    14281 agcgcgacgg caccgcgttt cgcgttgtcg cggaatggcg tgccgacggc gcgaagggcc
    14341 gcgccggcga gcgctttccg atcgaccact tcggcgagga ggaagcgcgg cgcgccgggc
    14401 tgcccatcgt catcgccgac acgcggacgc atcgtcgagc agaagcctgg cttgccgaag
    14461 gcaccggcgc cgtggtgacc gtgccgagcg tcacggcggg tcgcagcccg gcggcgctct
    14521 gggtcagcat gccggggccg cgcgcatggc atggcgacga aattgccctg atgcgcgaag
    14581 cggccgagcg cgtgtgggcc gagctcggac gggcgcgcgc ggaaagtgcg ctgcgcgaaa
    14641 gcgaggaacg cttccgtgcg atggcggaca gctcgccgct gctcgtctgg gtcgtcgatc
    14701 cgcacggacg cgtggtgttc gccaaccgcg cgtgccagga attctttggc cgcgcaccgg
    14761 cgcgtctcgg gcccgatggc tggcgcgcgt acctgcatcc cgacgacgcc gacggctaca
    14821 ccagcgaagt gctcgcagca ctcgccgcgc ggcgtccgtt cagcgtgatg ggccgcgtgc
    14881 gccgcagcga tggcgtctgg cgctgggtcc agtcgatcgg cgcgccgcgc ttctcggagg
    14941 ctggtgagtt cctcggcgcc gtcggcagca gccccgatgt caccgagctc atcgaagctt
    15001 ccgatgcatt gcgcgatgcc gaccggcgca aggacgagtt tctcgcgacg ctcgcgcacg
    15061 agcttcgcaa cccgcttgcc cccatccgcc aggccgcccg gctgtcgcgc tcgcccgagg
    15121 cgagcgatgc gcagcgccgc tggagccagg aagtgatcga gcggcaggtg aagcacatgg
    15181 cgctgctgct cgacgacctg ctcgacgtgt cgcgcatcac gcgcggcaag ctcgagttgc
    15241 gccgcgctcg cgtcgacctc gcctcggtcg ttgcgatggc gctcgaaacg gcgcatccgc
    15301 tgatcgacga tcgccggcag accgtgatcc acgatcttcc ggccgcaccg atcatcctgg
    15361 acgccgattc gatgcgcctc gcgcaagtgc ttgcgaacct gctcacgaat gcggccaaat
    15421 attcggaacc gggcggcatc atcgaacttg ccgcggtgcg cgaagtcgac gagatcagga
    15481 tcgacgtgaa ggacggcggc atcggcatcg agccaacgct gctgccgcgg gtattcgaga
    15541 tgttctccca gatgcacggt tcgctcgacc gcgccgaagg cgggctcggc atcggcctcg
    15601 ccctcgtgaa gggcctggtc gagctgcatg gcggccgcgt cgaggcgcac agcgcgggtc
    15661 tcggtcacgg cgcgcagttc agcgttcggc tgccgctgcc gccgcccggc gatgcgcccg
    15721 aagcccccgc gggcggcggc ggccacgctg aagcacccga catgcgccat gcacgaattc
    15781 ttgttgccga cgacaaccgc gacgcggcgt cgagcctcgc gacgctgctg atgctcgacg
    15841 gacatgatgt acgcgtcgcc aacgacggcg cgcgggcgct ggtcgaggcc gaatcgtttc
    15901 gcccgcacat cgcgctgctc gacatcggca tgccgatgca caacggctac gatgtggcgc
    15961 gcgcgatccg cgccgctcct tggggccggt cgatggtgct ggtagcggtc accgggtggg
    16021 gccagagcga ggacaagcgg cgcgcgaagg aagcgggctt cgaccatcac ttcacgaagc
    16081 cgctcgacct cgacgtgctc ggggcgttcg tgaccgatgc gcttgccagg cagcctgcgc
    16141 gctgaagcgc cgccttcggg cggcgcggcg gcgaacccgc gaacccgggg ggaacgttgg
    16201 ccgtcgtccg ccgtcgccgc ggcatacatt gcgctgtacg ttgcgctcga ctgggtgagc
    16261 ttcatcgacc cggtggcggc gtttgcaatc acgccatgga atccgccacc cggcctttcg
    16321 gtcatgttcc tgctccgccg cggcctgcgc caggcgccgt ggctgttcgt cgccgcgctg
    16381 gccgccgaat ttctcgttcg cggcgcgccg gccccgttgc cgctgttgat cgccgcgtcg
    16441 ctgctgctcg ccgcctcgta cacggccgtc accgccttgc tgacgcgcgt gctgcacgtc
    16501 gatcccgatc tcgcatcgct tcgcgatgca tcgatgctgg tcgtcaccgc tgcggttgcg
    16561 tgcggcgccc ttgcgctcgc attcgtcgcg ctcttcgccg gcagcggact gctgcccgcc
    16621 ggcgcgttct ggcgcagcgc gatgcagttc tggatcggcg atctcatcgg catcgtcgtc
    16681 acggcgcccg tgctgctcgt cgtcacgcgc aagcgcaacc ggcccgcgtt gcgcatcagc
    16741 gtcgaagggt tcgcccaggc ggccgcgatc ggcgttgcat tgtggatggt gttcggtgcg
    16801 ggccttcccg atgcaacgac gcttttctac gtgctgttcc tgcccgtgat ctggatcgcg
    16861 atgcggcacg ggatcgaagg cgccgtgttc ggtaccttgg cgacgcaggc cgggctcatc
    16921 gtcgcgatcg tggtcaccgg acaggggcgc gacgcggtgc tcgaattcca gttcctgatg
    16981 ctcgcgatcg caacgaccgc gttgctgctc ggcgcggcgg tctccgagcg gcgaacgatc
    17041 gagcggacac tgcgtgaaaa gcaggcgacg ctcgaccgca gcctgcggct tgcggcagcg
    17101 agcgagcttg cgtcttcgct cgcgcacgag ctcaaccagc cgctttcggc gatcatcgcg
    17161 tatctgcgcg catgcgagct catgctggcg cggccgggcg acgacacggc cgagcttcgc
    17221 gcgacgatga acaaggtgat cgcggaagcc agccgcgcgg gcagcgtcgt gcggcgcctg
    17281 cgcgatttct tccgctcggg atcggcacgg atcgagacgg tggcaccggc gacgctgatt
    17341 gccgaggcgt gcgcggcggt gatcgagcgc gcgcgccgca acggcgtcag cgtcgagcag
    17401 tcggcgcgaa acctgcccgg cgtgcgtgtc gaccgcgtgc agatcgaggc ggtgctcgcc
    17461 aatctgctcg gcaatgcgat cgacgcgttg cagcagcgcc cgggtgagcg cgaagtgcgc
    17521 atcgacgcgt cgcccgacac gccaggcttc gtgcgcattg ccgtcaccga caacggtccc
    17581 ggcgtcgatc cggagatcac gcagtcgctg ttcgacgcct tcgtgacgac gaaggcgagc
    17641 gggatggggc tcgggcttgc aatcagccgc tcgatcgtcg acgcgcacgg cggacgtctg
    17701 tggcacgagg cgcgcaatcc gggcagccgc ttctgcttca cggttccggc ggcatgagcg
    17761 ccgaagagcc gcctcgaggc gcgaactgcg ccccccgggg ggcagcgaag cagcgaagct
    17821 gcgagcgtgg ggggactttt tgattgccca acggccgctc gtcgcgatcg tcgacgacga
    17881 cgaggcggtg cgcgatgcgc tcggcctgct gctgcggctg cacgcgttcg acgtgcgcgc
    17941 cttcgcgtcg ggcgagcaat ttctcgcgtg ggcgggaacg cagccggtcg attgcgtgct
    18001 gctcgacctg cggatgagcg gcctgccggg gctcgacgtg caggccgcgc tcgcatcgcg
    18061 cgggctcgac tacccgatca tcgtcgtgac cgcgcacggc gacgttgcga cgacgcgcgc
    18121 ggcgctcaag gccggcgcgt tcgacttcat cgaaaagccg ttcgacgacg agctgctgct
    18181 gcgcacggtt cgtgcggcga gcgaagcgag cacccggcag cgcgcgcgcc gagcgcgacg
    18241 cgcgctttgc gcagagcatc gcccggttga cgccgcgcga gcgcgaagtg ctcgaccac
//
LOCUS       DASSVX010000070        16994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1185753, whole genome shotgun sequence.
ACCESSION   DASSVX010000070 DASSVX010000000
VERSION     DASSVX010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 16994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16994
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185753"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /gene="groL"
                     /locus_tag="VFO53_03225"
     CDS             <1..1312
                     /gene="groL"
                     /locus_tag="VFO53_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764708.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET8875317.1"
                     /translation="GIDKAVIAIVEELKKVSKPCSTSKEIAQVGSISANADESIGKTI
                     AEAMDKVGKEGVITVEDGKGLENELELVEGMQFDRGYISPYFINNPDRQVSLLEDPYI
                     LLHDKKISNIRELLPLLEQVAKSGKALLIIAEDVDSEALATLVVNNIRGILKTTAVKA
                     PGFGDRRKAMLEDIAILTGGTVIAEELGLKLENATLKDLGRAKRIEVGKDDTTIIDGA
                     GEKAAIEARVKNIRKQVEDATSDYDKEKLQERVAKLAGGVALVKVGAATEMEMKEKKA
                     RVEDALHATRAAVEEGIVPGGGVAYLRARANLKNLKGDNADQDAGIKIVLRAVEEPMR
                     QIVANAGDEPSVVVNRVVEGKGNFGFNAQTGEYGDLVAMGVVDPTKVTRFALQNAASV
                     AGLMLTTDAMIAELPQDDRGHGGAMGGGGMGGMGGMGGMGDMGM"
     gene            1619..1957
                     /locus_tag="VFO53_03230"
     CDS             1619..1957
                     /locus_tag="VFO53_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET8875318.1"
                     /translation="METTKALAALAGLAQDTRLRLFRLLVEHGPEGQSPGAIAEELGL
                     ADATLSFHLKELARAGLVSARRVGRFIYYSANYRTMNGLVAYLTENCCRGASCASACA
                     PVRKPKRKAS"
     gene            1954..2436
                     /locus_tag="VFO53_03235"
     CDS             1954..2436
                     /locus_tag="VFO53_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HET8875319.1"
                     /translation="MKRFHVHVAVDDLAESIRFYSMLFGAEPAVRKTDYAKWMVDDPR
                     INFAISSRGSASGVNHLGFQVDSDDELKGMRERLAAADAGLTEEADTHCCYAVSDKYW
                     VTDPQGIAWETYHTLGSIPVFGEAAGAADDQGRSGCCTPAEANVAAPVPATARARCCT
                     "
     gene            complement(2564..3544)
                     /locus_tag="VFO53_03240"
     CDS             complement(2564..3544)
                     /locus_tag="VFO53_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8875320.1"
                     /translation="MTVIDVHTHMFTAKWLELLKKEGGLYSIKTRPDGQQEIFRGDTP
                     VVIPQRGHFDYDLRMRHMDAAGIDVSIVSLTCPNVYWGGEDVSCRAARESNDTMAEAQ
                     RTYGDRIRWFTSLPWEYPQRALEELGRTCGNGAVGVMVLANVSGRSLTDPLFEPVWAE
                     IDRRGLPVLVHPTDPPGVDMMDMTRFDLSWSVGFMFDTTLAITRMIFDGFFDRYPNLR
                     LIASHGGGTLPYLVGRFEKGDEVEIPSRRRMRRKPADYLRHIYYDSITYDPGALQYLV
                     SVVGADHVMLGTDWPHQVHDTKGAFANTARLPDGQMRAIRAQNAQRIFGL"
     gene            complement(3569..4363)
                     /locus_tag="VFO53_03245"
     CDS             complement(3569..4363)
                     /locus_tag="VFO53_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET8875321.1"
                     /translation="MTTGATPRENATIASLVHINKWYGAYHALRDVDLQVASGERVVI
                     CGPSGSGKSTLVRTVNLLEPFQQGRLHVCGVDVGADGITRAARLAVNRRVGMVFQQFN
                     LFPHMSALQNLTVGPVFGRGVSRKEADELARHYLQRVGLLDHAHKHPAQLSGGQQQRV
                     AIARALCTRPQLVLFDEPTASLDPEMVSEVLQTMEGLAREGMTMIVVTHEMGFARRVA
                     DRVLFMDQGQIIEAAPPADFFDRPRSPRLRSFLGQVLAHLNPPVDV"
     gene            complement(4380..5471)
                     /locus_tag="VFO53_03250"
     CDS             complement(4380..5471)
                     /locus_tag="VFO53_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET8875322.1"
                     /translation="MHYVRSIPIGARAAPTPAGAALRWRRDFFASPAIGAVTLALLVL
                     LTVLGWRALQWALVNAHWVGASSAACPGDAGACWAFVTARWKPWLVGNYPVSELWRAW
                     SCFAGFALFWTWVVRRSDAASMQRVLLGFVALPVAFFLLLRGGGPLPVVVPTQWGGLL
                     LTLVVTLVTFATALPVGLCLALGRRSRLPVVRWLCGAFVEGMRAVPLLAVLFIAATLL
                     PLFLPRGANIDLFARAILAFALFNAAMAAEVFRGGLQSIGRGQREAATTVGLSDFAAL
                     RLIVLPQAVTAVVPALVNIMIAVIKETTLLSVIGVNDLLGAIENGARSPDWMGESNIL
                     TSGYVFLALAYLTVCYGLSRYSRRLEARR"
     gene            complement(5531..6514)
                     /locus_tag="VFO53_03255"
     CDS             complement(5531..6514)
                     /locus_tag="VFO53_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8875323.1"
                     /translation="MSRAVLIERLPGYLGQAVLLAAVAWLVWRGVANFQANMRALGVS
                     VGFGFLHTQAGFEIAQALIAFSPASTYLDAVIVATLNTLLVVALAAVLGTLIGLVVAF
                     GRLAPYGLIHGAASAYVEVFRNIPLLLQILFWYFSVIALLPPVADSLHLGAVLLNNRG
                     LFLPRLLLHGQFGLDRPQVEGLAVGGGFAILPELMALTLGLSIYNSTFLAEIFRAGIL
                     SVPRGQIEAAATVGLRGVRAAWLITLPQALRVALPAAGGQYQTLAKASSLAAAIGYPD
                     IMQIIGGTIASQTSQALEAMALVVLLYGAINLAIALIVGLANRRLLAREGR"
     gene            complement(6589..7620)
                     /locus_tag="VFO53_03260"
     CDS             complement(6589..7620)
                     /locus_tag="VFO53_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET8875324.1"
                     /translation="MRFQCTLSLAGRSLLGLAAASVLAGAARADVLDDVKAHGVLKCG
                     VTLAAAGFSAENNAGKREGFDIDLCKAIAAATLGDASKIQLTPMDLKTAFAGLPTGVV
                     DVLTHRFTWTQSRDVGGLKFPKVMFFDGQGFVVTKKSRASRIADLNNATICTLQGSTS
                     ELIVASYFGAHKLKYKIVTFASAEQAWDAFVGKRCDTVINDRSGLAARLAKLPNHDDY
                     KVLGETVSNEPIGPTVAQGQQRWEDLVRFTLNALVAAEELGVTQANADSERSSQNPDV
                     QRLLGVTGDFGTKLGVANDYAYRAIKAVGNYGEIWDRNVGPKTPLRLERGRNALVANG
                     GLMMSIPIR"
     gene            complement(7842..8573)
                     /locus_tag="VFO53_03265"
     CDS             complement(7842..8573)
                     /locus_tag="VFO53_03265"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET8875325.1"
                     /translation="MRASILLLYGLVVYLFFVATFLYAIGFTGNLVVPKSIDTGAAVP
                     LAEALVVDLLLLGLFAAQHSVMARRSFKRWWTRIVPPIVERSTYVLAATLALALLLWQ
                     WRPIGAPVVWRIESDTGVHIVWAVFWLGWAVLLISTFLINHFELFGLRQVFARVAGSE
                     PPEQEFRTPFFYRYVRHPLYLGFLLGFWATPVMTAGHLLFSIATTGYILVGIWFEERD
                     LVAQFGEPYRTYRSQVGMLLPHRRA"
     gene            8776..9657
                     /locus_tag="VFO53_03270"
     CDS             8776..9657
                     /locus_tag="VFO53_03270"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8875326.1"
                     /translation="MARLGFVGLGVMGSRVVKRLLAAGHAVTGYNRTIAKAQWLVDAG
                     LTLAGSPRAVAEAADITFTMVTNTAALRSVMEGPAGLLAGFGAGKIHVDMSTVSPSAS
                     RELAQQVEAKGARMLDAPVSGSVVTLEEGKLTFMVGGERSVFERLKPIFLDVGTRATY
                     VGGNGLAVSMKIATNLSLAVQMLAFSEGVLLAEKSGIARDVAVDVLVNSVIGSPMVKY
                     RGPFVLGMPDEAWFDCNMMQKDMNLALEMGRQLGVPLPTTATTNEFLTAARGMGWEGQ
                     DFAVVFDVLARLSGVTR"
     gene            complement(9683..10456)
                     /locus_tag="VFO53_03275"
     CDS             complement(9683..10456)
                     /locus_tag="VFO53_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HET8875327.1"
                     /translation="MQRLRTPTAVIADDERLMRDQIVAQLSQAWPELRVVGEASNGRE
                     AVAAVLRLEPDIVFLDIRMPEMDGVEAARALAGRAHVVFVTAYDQYAISAFEQGAVDY
                     LLKPADAERLALTCQRLRARLAQAPEPMNDLLAQLSQQLAAGGIQPRGYLRWVQASVG
                     AHLRMIPTSDILFFRAEDKYTRVQTPRFEALIRKPIKELIDELDPAEFWQIHRATVVR
                     VDAIEQVGRNLRGNQVVHVKGSDEKLEVSRSFNHLFKQM"
     gene            complement(10468..11412)
                     /locus_tag="VFO53_03280"
     CDS             complement(10468..11412)
                     /locus_tag="VFO53_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET8875328.1"
                     /translation="MDTDPRMAGTGARSERDAAGLSSRSASTRRPLQDIRDSVVAMWW
                     GFFDWLAVASWKTLTFVSCLGLILAGILEHPVPALVLIIASFITKAVAGGKRRAEVTA
                     DEANRRAETEQLERTILEARMQALQAQIEPHFLFNTLASIDQLILTDPPRASSMQRSL
                     IRYLRSAMPQMREGGRPSLGQQVDLCRAFLDIMGMRMEERMQAAVTVPDGLKSAVFPS
                     MMLQTLVENAIRHGLEPKAEGGRLEIGAEVVDGQLAVHVRDTGVGFAGAGSDGVGLAN
                     IRARLSALYNERAELVISVPPAGGTCATIKLPYEVAPA"
     gene            11735..12811
                     /locus_tag="VFO53_03285"
     CDS             11735..12811
                     /locus_tag="VFO53_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HET8875329.1"
                     /translation="MTATSPIALAARQFAVVACALALSTPVFADAGNYEVTKLVADLP
                     GVAANTDPNLVNGWGIAMRATSPIWVSNNATGTSTLYNGLGVQISPPSPVIIPAANAS
                     GRGVPTGITSNPSTSDFLLGGPGTAAIFVWATEDGAIAAWNGGPAASVKFAAKDGAVY
                     KGLALAGNGSSFRLYAADFHNGKIDVLDNTFTPTSVAGGFVDPRIPAGFAPFNIMNLQ
                     GNLYVAYALREPGGDDEVAGAGLGFVDVFDADGFLVARAATRGKLNAPWGMAIAPAGF
                     GRFSNHLLVGNFGDGTINAYDVKNFTFAGQLHASNGRTLVIDGLWGIAFGNGFQRQPT
                     DALFFAAGPDDETHGLYGRIDPAP"
     gene            complement(12864..14207)
                     /locus_tag="VFO53_03290"
     CDS             complement(12864..14207)
                     /locus_tag="VFO53_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET8875330.1"
                     /translation="MSNVSEEQARAHMHKLLAAMQGAGGSDLFIAADFPPSMKAHGQM
                     QPLTAQKMSGQMTRMLAHALMNERQREEFARDLECNFAISVPGLSRFRVNILVQQQQV
                     GMVIRTIASEMPTFEKLGLPETLKDIVMHKRGLVLVVGATGSGKSTSLAAMIDHRNST
                     SSGHIITIEDPVEYVHASKQSLVTHREVGVDTHSWHGALKNTLRQAPDVILIGEIRDA
                     ETMEHAITFAETGHLCLGTLHANNANQTLDRIVNFFPEERRNQLLMDLSANLRAIVSQ
                     RLVRTQDGEGRKAAIEILINTPTIADRIFKGEFHELKAIMAKSRELGMRTFDWALFEL
                     YNDGHIGYEEAIRNADSANELRLNIKLRSERGEPATAGGLSLSIDEAPSGETLEQQRQ
                     QELVKQQEKKRQLEQERLVALHAEKKLQMQKKRELEDLELAALRAQNKSREAEAA"
     gene            complement(14323..14823)
                     /locus_tag="VFO53_03295"
     CDS             complement(14323..14823)
                     /locus_tag="VFO53_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET8875331.1"
                     /translation="MSYINGNLLEGEHVVFRTRLHWKLLVGPGVFLLATLVPPAWLYL
                     QRTWTDWVLIAPLVGLIVLLAAIVKRMSSDFAVTNKRVMMKTGIFNTRSVELLLNKIE
                     AIAVNQTLAGRMLGYGHIVVTGSGGTGETFSDIQAPLEFRRAVQSVTDAQAIPLEHAS
                     RIPASG"
     gene            complement(14820..15299)
                     /locus_tag="VFO53_03300"
     CDS             complement(14820..15299)
                     /locus_tag="VFO53_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875332.1"
                     /translation="MRARCRASIDQGPLTRPRLNSVESSRSNHDRCGEAARRHPIEET
                     GMDRNARLRIARSLAAALLAFGISGGASANRGGGGGGGDNGDSSMNPFTGDSYAYFHG
                     GHNLGEQGTIFPDHAYRTPSKKAQEPKAARAPASGPTADTGAARTRPSDVPQNPAPR"
     gene            complement(15271..16698)
                     /locus_tag="VFO53_03305"
     CDS             complement(15271..16698)
                     /locus_tag="VFO53_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8875333.1"
                     /translation="MTDDRIVESAVPARLDALRWSRFHTLVIAALGITWVLDGLEVTL
                     AGAVSGALKASPTLHFQDAQVGLSASAYLAGAVLGALVFGWLTDRFGRKRLFFVTLGV
                     YVLGTGATALSTDFATFALCRFITGAGIGGEYSAINSAIQELIPARYRGRTDLGVNGS
                     FWLGAVLGAIGSSLLLAPGRLPLDVGWRVCFGLGALLGLAILALRRHLPESPRWLLLH
                     GREAEAERIAADVERMCASAHPATSLRSLRLRIRARSANFVDLGMTLFAHYPRRALLG
                     LVLMTAQAFCYNAIFFTYALVLGRFYAVPPEDIGLYLLPFAIGNFAGPLVLGRYFDTW
                     GRRQMIATTYALAAVLLGLTAVLFAAGLLSAATQTLAWTLVFFFASAAASSAYLVVSE
                     SFPLEVRALSIAFFYALGTGIGGIGAPWLFGVLIGTGQPLAIASGYLLGALLMLIAAA
                     FAWRLGIAAERKPLEDVARPLSCVD"
     gene            complement(16695..>16994)
                     /locus_tag="VFO53_03310"
     CDS             complement(16695..>16994)
                     /locus_tag="VFO53_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014504003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875334.1"
                     /translation="SSGVCPACRHYLRFDAGGDAAPVPAITPLRIEGAIRHSADADPW
                     EYTVVVTIRNGRGEEIARKLIGVGALDANEQCTFALSVEAVTARGKKPGKGGTRH"
BASE COUNT         2983 a         5560 c         5724 g         2727 t
ORIGIN      
        1 cggcatcgac aaggcggtca tcgccatcgt cgaggagctg aagaaggtga gcaagccgtg
       61 ctcgaccagc aaggaaatcg cgcaggtcgg ctcgatctcg gccaacgccg acgaatcgat
      121 cggcaagacg atcgccgagg caatggacaa ggtcggcaag gaaggcgtga tcaccgtcga
      181 ggacggcaag ggtctcgaga acgagctcga gctcgtcgaa ggcatgcagt tcgaccgcgg
      241 ttacatctcg ccgtacttca tcaacaatcc cgaccgccag gtgtcgctgc tcgaggatcc
      301 gtacatcctg ctgcacgaca agaagatcag caacatccgt gagttgttgc cgctcctcga
      361 gcaggtggcg aagtccggca aggcacttct catcatcgcc gaggacgtcg acagcgaggc
      421 actggccacg ctggtcgtca acaacatccg cggcatcctg aagacgaccg ccgtcaaggc
      481 gccaggcttc ggcgatcggc gcaaggcgat gctcgaggac attgcgatcc tcaccggcgg
      541 cacggtgatc gcggaagagc tgggcctcaa gctcgagaac gcaacgctca aggatcttgg
      601 ccgcgccaag cggatcgagg tgggcaagga cgacacgacg atcatcgacg gtgccggtga
      661 aaaggcggcg atcgaggcgc gggtcaagaa catccgcaag caggtcgagg atgcgacgtc
      721 cgattacgac aaggaaaagc tgcaggaacg cgtggcgaag ctcgccggcg gtgtcgcgct
      781 ggtcaaggtc ggcgcggcaa ccgaaatgga aatgaaggaa aagaaagcgc gcgtcgagga
      841 tgcgctgcac gcaacccgtg cggccgtcga ggaaggcatc gtcccgggcg gcggcgtcgc
      901 gtatctgcgt gcgcgtgcga acctcaagaa cctgaagggc gacaacgccg atcaggacgc
      961 cggcatcaag atcgtgcttc gcgcggtcga ggagccgatg cgccagatcg tggccaatgc
     1021 gggcgacgag ccgtcggtcg tcgtgaacag agtggtggaa ggcaagggca atttcggctt
     1081 caacgcgcag accggcgaat atggcgatct cgtcgcgatg ggcgtcgtcg acccgaccaa
     1141 ggtgacgcgc tttgcactgc agaacgcggc atcggtggcc ggactgatgc tcacgaccga
     1201 cgcgatgatt gccgagctgc cgcaggacga ccggggtcac gggggtgcca tgggtggcgg
     1261 cggcatgggc ggcatgggtg ggatgggcgg catgggcgac atgggcatgt gacgcgaatg
     1321 tgacgccgca agccgtgcaa aggccccggt ttttcggggc ctttgctttt gcgtttgggc
     1381 ctatagtgat ggatcacgat cgaccggagc gaacgatgtc acatcagccc ccgaagcaaa
     1441 gcaagaagaa gccgcagcat acggccaagg agaagaagtc gatcaagcag cagaagaagc
     1501 acgcgagcga ccaggcgccg ttcatcaagc aatgacgcgc gcgcattgcg cgccccggcg
     1561 ggacggtgcg ccgggcccgt gacttgattt tcgcctacgt ttccattatg ttggaaaggt
     1621 ggaaacgacg aaagcgcttg cggcactggc cgggctggca caggacacgc gtcttcggct
     1681 cttccgcctt ctggtcgagc acgggcccga aggccagtcg ccgggtgcca tcgccgaaga
     1741 gctcggcctc gccgacgcca cgctgtcgtt tcatctgaag gagcttgctc gtgccggcct
     1801 ggtatccgcg cgccgggtcg gtcgcttcat ctattacagc gcgaactatc gaaccatgaa
     1861 cgggctcgtc gcgtatctca ccgaaaattg ctgtcgcggt gcatcctgcg ccagcgcgtg
     1921 cgcacccgtt cgcaagccga agaggaaggc atcatgaagc gtttccatgt gcacgtcgcg
     1981 gtcgacgatc ttgcggaaag catccggttc tattcgatgc tttttggtgc cgagcccgcg
     2041 gtgcgaaaaa ccgattacgc caagtggatg gtcgacgacc cgcggatcaa ttttgcgatc
     2101 tcgtcgcgcg gttccgccag cggcgtcaac cacctcggct tccaggtcga cagcgacgac
     2161 gagctcaagg ggatgcgcga aagactcgcc gccgccgacg ccggcttgac cgaggaagcg
     2221 gacacgcact gttgttacgc cgtgtccgac aaatactggg tgaccgatcc ccagggcatc
     2281 gcatgggaga cgtatcacac gctgggctcg atcccggtat tcggcgaagc ggcgggcgca
     2341 gcggacgatc aggggcggtc gggctgctgt actcctgcag aggcgaacgt ggcggcgccg
     2401 gttcccgcca cggcccgggc acgctgctgc acctgaccgc taatgtgcga cgcgggtgag
     2461 ggtcaagggc acgctggttt tcgacaccga cggcagcgcc tcggccggta actcggttct
     2521 ggtcggtggt ctcgcggtcg gcgcaatcgt gcccggaaga gcgctatagc ccgaagatgc
     2581 gttgcgcgtt ctgtgcgcgg atggcgcgca tctgaccgtc cggcaggcgc gccgtgttcg
     2641 cgaacgcacc cttcgtgtcg tgcacctggt gcggccaatc ggtgccgagc atcacgtgat
     2701 cggcgccgac aaccgaaacg agatactgca gcgcgccggg gtcgtaggtg atgctgtcgt
     2761 agtagatgtg ccgcaggtag tccgcaggct tgcgccgcat gcgtcgacgg ctcgggatct
     2821 ccacttcgtc gcccttttcg aagcggccga cgagatacgg gagcgtcccg ccgccgtgcg
     2881 aggcgatgag ccgcagattc gggtagcgat cgaagaatcc gtcgaagatc attcgcgtga
     2941 tggccaatgt cgtatcgaac atgaagccga ccgaccagct caaatcgaat cgcgtcatgt
     3001 ccatcatgtc gacgccgggc ggatcggtgg gatgcacgag caccgggaga ccgcggcggt
     3061 cgatctcggc ccaaacgggt tcgaacagcg ggtcggtcag gctgcgtccg gagacattgg
     3121 ccagcaccat gacgccgacg gcaccgttgc cgcacgtgcg acccaattcc tcgagtgcgc
     3181 gctgcggata ttcccatggc agcgacgtga accagcgaat gcgatcgccg tacgtgcgct
     3241 gcgcctccgc catcgtgtcg ttcgactcgc gcgcggcgcg gcagctcacg tcctcgccgc
     3301 cccaatagac gttcggacac gtgagcgaga cgatcgacac gtcgatgccc gcggcatcca
     3361 tgtgtctcat ccgcaggtcg tagtcgaaat ggccgcgctg cggaatcacg accggcgtgt
     3421 ctcccctgaa gatttcctgc tggccgtcgg ggcgcgtctt gatgctgtag aggccgcctt
     3481 ccttcttcaa cagctcgagc cacttcgcgg tgaacatgtg ggtgtgaacg tcgatcacgg
     3541 tcatcggctg ggtctccaga ttcgggtttc aaacgtcgac gggcggattc aggtgcgcga
     3601 gcacctggcc gaggaacgat cgcagccgcg ggctgcgcgg ccggtcgaag aaatcggcgg
     3661 gcggcgcggc ttcgatgatc tgcccctggt ccatgaatag cacacggtcg gccacacgcc
     3721 gcgcgaagcc catctcgtgc gtgacgacga tcatcgtcat gccttcgcgc gccaggccct
     3781 ccatcgtctg cagcacttcg ctcaccatct ccgggtcgag cgaggcggtg ggctcgtcga
     3841 acagcacgag ctgcgggcgg gtgcatagcg cgcgtgcgat cgcgacgcgc tgctgctgcc
     3901 cgccggaaag ttgcgccggg tgcttgtgcg catggtcgag caggccgacg cgctgcaggt
     3961 agtgtcgcgc gagctcgtcg gcttccttgc ggctcacgcc gcggccaaag accgggccga
     4021 cggtgagatt ctgcagcgcg ctcatgtgcg ggaacaggtt gaactgctgg aacaccatgc
     4081 cgacgcgccg attcacggcg agccgcgccg cgcgcgtgat cccatccgcg ccgacgtcca
     4141 cgccgcagac gtgcagacga ccctgctgga acggctccag cagattcacg gttcgcacca
     4201 gcgtcgattt gcccgagccc gaggggccgc agatcacgac acgctcgccg gaagcgacct
     4261 gcaggtccac gtcgcgcagc gcgtggtagg cgccgtacca cttgttgatg tgcacgagcg
     4321 acgcgatcgt cgcgttctcg cgcggtgtcg cacccgtcgt catcagcgct cgcgcaagtt
     4381 caacgccgtg cttcgaggcg ccggctgtag cgcgacagcc cgtagcagac cgtgagatag
     4441 gcgagcgcaa gaaagacgta tcccgacgtg aggatgttcg actcgcccat ccagtccggc
     4501 gatctggctc cgttctcgat ggcgccaagc aggtcgttga cgccgatgac cgacagcagc
     4561 gtggtctcct tgatcactgc gatcatgatg ttcacgagcg cgggcaccac cgcggtcacg
     4621 gcctgcggca gaacgatgag ccgcagcgcg gcgaagtcgg acaggcccac cgtcgtcgcc
     4681 gcttcgcgct gcccgcgtcc gatcgactgc agtccgccgc ggaaaacttc ggccgccatc
     4741 gctgcgttga aaagcgcgaa cgcgaggatg gcgcgcgcga aaaggtcgat gttcgcaccc
     4801 ctgggcagga acagcggcag cagcgtcgcg gcgatgaaca gcacggcgag caacggcacg
     4861 gcgcgcatcc cttcgacgaa cgcgccgcag agccagcgca cgacgggcaa ccgcgagcgg
     4921 cgaccgaggg ccaggcacag gccgacgggc agtgcggtcg cgaacgtcac gagcgtcacc
     4981 acgagggtga gcagcaaccc gccccattgc gtcggcacga cgaccggtaa tggaccgccg
     5041 ccccgcagca gcaggaaaaa cgccacgggc agcgcgacga agccgagcag cacgcgctgc
     5101 atgctcgccg catccgaacg acgcacgacc caggtccaga aaagcgcgaa tcccgcgaaa
     5161 caactccatg ctcgccacag ctcgctaacg ggatagttgc ccacgagcca cggcttccag
     5221 cgtgcggtga cgaacgccca gcatgcaccg gcatcgccgg gacaggctgc gctcgaagcc
     5281 ccgacccagt gcgcgttcac gagggcccat tgcagcgcac gccagccgag cacggtgagc
     5341 agcacgagca gcgcgagcgt tacggcgccg atcgcaggcg aggcgaagaa gtcgcgacgc
     5401 cagcgcagcg ctgcgccggc cggcgttggt gcagcgcgcg caccgatcgg tatcgaccgt
     5461 acgtagtgca tggcatcgtc gtgctgcatg cggttcgaca cgtcggctgt ccgctcgcgc
     5521 gccacgttcg tcagcgcccc tcgcgcgcga gcagccgccg attggcgagg cccacgatga
     5581 gcgcgatggc gaggttgatc gcgccgtaca acagaaccac cagcgccatc gcttcgagcg
     5641 cctggctcgt ctggcttgcg atggtgccgc cgatgatctg catgatgtcc gggtaaccga
     5701 tcgccgcggc gagcgacgaa gccttcgcca gcgtctggta ctgcccgccg gccgcgggca
     5761 acgccacacg cagcgcctgc ggcagcgtga tcagccacgc cgcgcgcacg ccgcgcaggc
     5821 ccaccgtggc cgccgcttcg atctgcccgc gcggcaccga aaggatgccg gcacgaaata
     5881 tctcggccag gaacgtgctg ttgtagatcg acagacccag cgtcagcgcc atgagctcgg
     5941 gcaggatcgc gaagccgccg ccgacggcta gcccctcgac ctgcgggcgg tcgagaccga
     6001 actgtccgtg caaaagcagg cgcggcagga acagtccccg gttgttcagc agcaccgcgc
     6061 cgaggtgcag gctgtcggcg acgggaggca gcagcgcaat caccgagaag taccagaaca
     6121 ggatctgcag caacagcggg atgttgcgga acacctcgac gtaggccgac gcggcgccgt
     6181 ggatcaatcc atacggcgcg agcctgccga atgcgacgac gagtccgatc agcgtgccga
     6241 gcacggccgc aagcgccacg acgagcagcg tgttgagcgt ggcgacgatg acggcatcga
     6301 gatacgtcga cgccgggctg aatgcgatca gcgcctgcgc gatctcgaag cccgcctgcg
     6361 tgtgcaggaa gccgaagccg accgacacgc cgagcgcgcg catgttggcc tggaagttcg
     6421 cgacgccacg ccacacgagc cacgcgacgg cagcgagcag cacggcctgc ccgaggtaac
     6481 ccggcaggcg ctcgatcaga accgcgcgcg acattcttgc catcgaagcg ccgtcgtgct
     6541 gctggaggaa cgccgggagc cgcgctcttg cgccttcgcg tacgcctttc agcggatcgg
     6601 aatcgacatc atgaggccgc cgttcgcgac cagcgcgttg cgtccgcgct cgaggcgcag
     6661 cggcgtcttc ggtccgacgt tgcggtccca gatttccccg tagttgccta cggccttgat
     6721 cgcccggtag gcgtagtcgt tggcaacgcc gagcttggtg ccgaagtcgc cggtgacgcc
     6781 gagcagccgc tgcacgtcgg gattttgcga gcttcgctcg ctatcggcgt tggcctgggt
     6841 cacacccaat tcctccgccg ccaccagcgc attcaacgtg aagcggacga gatcctccca
     6901 acgctgctgt ccctgcgcca ccgtcgggcc gatcggctca ttggacaccg tttccccgag
     6961 caccttgtag tcgtcgtggt tcggcagttt cgcaagccgc gcagcgagcc ccgagcggtc
     7021 gttgatcacg gtgtcgcagc gcttgccgac gaaggcgtcc catgcctgct cggccgacgc
     7081 gaacgtgacg atcttgtact tgagcttgtg cgcgccgaag tagctcgcga cgatcaactc
     7141 cgacgtgctg ccttgcagcg tgcagatggt cgcgttgttg agatcggcga tccggctcgc
     7201 ccgggatttc ttcgtcacca cgaaaccctg gccgtcgaag aacatgacct tcgggaactt
     7261 gaggccgccc acgtcgcggc tctgcgtcca ggtgaagcga tgcgtgagca cgtcgacgac
     7321 gcccgtcggc agtcccgcga acgcggtctt caggtccatc ggcgttaact ggatcttgct
     7381 cgcatcgccc agcgtcgccg cggcgatggc cttgcacagg tcgatgtcga agccctcgcg
     7441 cttgccggcg ttgttctcgg cggaaaagcc cgcggcggca agcgtcacgc cgcacttcag
     7501 tacgccatgc gccttgacgt cgtcgagaac gtccgcgcgc gcagcgccgg caaggacgct
     7561 cgcagccgcg aggcccagca acgatcggcc cgcaagggaa agcgtgcatt gaaagcgcat
     7621 ctggattgcc tccgtcttcg aaaaggggcg atgccgggcg acgcggccga ccaatgcaat
     7681 gcgcgggctc gggccgccgg gtgctgcgcg gcgcattgtc gcatcttgtt cccggcgcaa
     7741 cgcccaaacc tgcgcagctc gcgtctcgcg cgctcgccgc gtgcaccggc ggtcgatacg
     7801 ccccggggag gcgccggggc ggaaccgcag cgcagcgccc gttacgcccg gcgatgcggc
     7861 agcagcatgc cgacctggct gcgataggtg cggtacggct cgccgaactg cgcgacgaga
     7921 tcgcgctcct cgaaccagat gccgaccagg atgtagccgg tggtcgcgat cgagaacagc
     7981 aggtgtcccg ccgtcatgac cggcgtcgcc cagaatccga gcaggaagcc caggtagagc
     8041 ggatggcgca cgtagcgata gaagaacggc gtcctgaatt cctgctcggg cggctcgctg
     8101 ccggcaaccc gcgcgaacac ctggcgcaat ccgaagagct cgaaatggtt gatgaggaac
     8161 gtgctgatca gcagcaccgc ccagcccagc cagaagaccg cccagacgat gtgcacgccg
     8221 gtatcgctct cgattcgcca cacgaccggc gcgccgatcg gccgccattg ccacagcagc
     8281 agcgcaagcg ccagcgtagc ggcgaggaca taggtgctgc gctccacgat cggcggcacg
     8341 atccgcgtcc accagcgctt gaacgagcgg cgtgccatca cgctgtgctg cgcggcgaag
     8401 aggccgagca gcagaaggtc gacgaccagt gcttcggcga gcgggaccgc cgcgcccgtg
     8461 tcgatcgact tcggcacgac gagattgccg gtgaagccga tcgcataaag aaacgtcgcg
     8521 acgaagaaca ggtaaacgac gaggccgtac aagagcagga tcgatgcgcg catggaagtg
     8581 tcccctcggt ttgtcaggcg tcgagcgcgc gcgagcgcgg cggacgacga caggaagtct
     8641 attgcgacgg cgcagcgcga agatgtcacc gtcgtctcga tgtcgtttca tttgtttcgc
     8701 cggtgctcga gccatcgcat ccgtcgattc gcgggccgcc tacaatgatc ggcttcgttt
     8761 ccaggaggat ctgacatggc acgtctcggc ttcgtcggtc tcggcgtaat gggcagccgc
     8821 gtcgtcaagc gtctgctcgc tgcggggcac gcggtcaccg gctacaaccg cacgattgca
     8881 aaggcgcaat ggctggtgga cgcgggcctg acgctcgcgg gctccccacg cgcggtcgcc
     8941 gaagcggcgg acatcacgtt cacgatggtg acgaacacgg ccgcactgcg cagcgtgatg
     9001 gaggggcccg cgggactgct cgccggcttc ggcgccggca agattcacgt cgacatgagc
     9061 acggtcagtc cgagcgcaag ccgcgagctc gcgcagcagg tggaagcgaa gggcgcgcgc
     9121 atgctcgacg cgccggtgtc gggaagcgtc gtcacgctcg aggagggcaa gctcacgttc
     9181 atggtgggtg gcgagcgcag cgtcttcgaa aggctgaagc cgatcttcct cgacgtcggg
     9241 acgcgggcca cctacgtcgg cggcaatgga ttggcggtgt cgatgaagat cgcgacgaac
     9301 ctgagccttg ccgtgcagat gctcgcgttc agcgaaggcg tgttgctcgc ggagaagagc
     9361 ggcatcgccc gcgacgtcgc ggtggacgtg ctcgtcaaca gcgtgatcgg ctcgccgatg
     9421 gtgaagtacc gcggtccgtt cgtgctcggg atgcccgacg aggcgtggtt cgactgcaac
     9481 atgatgcaga aggacatgaa cctcgccctc gagatggggc gccagctggg cgtgccgctg
     9541 ccgaccaccg cgaccaccaa cgaatttctc accgccgcgc gcggcatggg atgggaaggg
     9601 caggacttcg cggtcgtctt cgacgtgctc gcgcgcctct cgggcgttac gcggtagccg
     9661 ggcaggcgag cgccgtgcgc ggctacatct gcttgaacag atggttgaag ctgcggctca
     9721 cttcgagttt ttcgtcgctc cccttgacgt gcacgacctg gttgccgcgc aagttgcggc
     9781 cgacctgctc gatggcatcg acgcgaacga cggtcgcgcg gtggatttgc cagaattcgg
     9841 ccgggtcgag ctcgtcgatc agctccttga tcggcttgcg gatcagcgcc tcgaaacgcg
     9901 gcgtctgcac gcgcgtgtac ttgtcctcgg cgcggaagaa caggatgtcg cttgtcggga
     9961 tcatgcgcag gtgtgcgccc acgctcgcct gcacccagcg cagatagcca cggggctgaa
    10021 tcccccctgc ggcgagctgc tgcgatagct gcgcgagaag atcgttcatc ggctcggggg
    10081 cctgcgcgag ccgcgcccgc aggcgctgac aggtcagtgc gagccgttcc gcgtcggccg
    10141 gcttcaacag ataatcgacc gccccttgct cgaaggcgct gatcgcatac tggtcatagg
    10201 cggtgacgaa gacgacgtgc gctcggccgg cgagcgctcg cgcggcctcg acaccgtcca
    10261 tttccggcat cctgatatcg aggaagacga tgtcgggttc gagtcgcagc acggccgcga
    10321 ccgcctcgcg gccgttgctc gcttcgccca cgacgcgcag ctccggccac gcctggctca
    10381 attgcgccac gatctggtcg cgcatcaggc gctcgtcgtc ggcgatgacc gcggtggggg
    10441 tgcgcaggcg ctgcatgctc gactcgttca ggcgggtgca acttcgtaag gtagcttgat
    10501 ggtcgcgcag gtgccgccgg ccggcggcac gctgatgacc agctcggccc gctcgttgta
    10561 caaggcgctg agtcgggccc ggatgttcgc gagaccgacc ccatcgctgc ccgcgccggc
    10621 aaagccgacg ccggtgtcgc gcacatgaac ggcgagttgc ccgtcgacga cttcggcgcc
    10681 gatctcgagc cggccgccct cggctttcgg ctcgaggccg tgccggatgg cattttcgac
    10741 cagcgtctgc agcatcatcg atggaaacac cgccgacttg agcccgtcgg gaacggtgac
    10801 cgccgcctgc atgcgctctt ccatgcgcat ccccatgatg tcgagaaacg cacggcaaag
    10861 gtcgacctgc tggccaagcg acggccgccc gccttcgcgc atctgcggca tcgccgagcg
    10921 gaggtagcgg atgaggctcc gctgcatgct cgacgcgcgc ggcggatcgg tcaggatgag
    10981 ctgatcgatg ctggcgagcg tgttgaagag gaagtgcggc tcgatctgcg cttgcagcgc
    11041 ctgcatgcgc gcttcgagga tggtgcgctc gagctgttcg gtttcggcgc gccggttggc
    11101 ttcgtccgcg gtcacttccg ctctgcgctt gccgccggcc accgccttcg tgatgaacga
    11161 ggcgatgatg aggaccagcg ctggcacggg atgctcgagg atgccggcga ggatcagtcc
    11221 gaggcaggag acgaacgtca gcgtcttcca cgacgcgacg gcaagccagt cgaagaagcc
    11281 ccaccacatc gcgacgacgc tatcgcgaat gtcctgcagc ggccgccgcg tcgacgcgct
    11341 gcgtgatgaa agaccggctg catcacgctc gctgcgcgcg ccggtgcctg ccatgcgggg
    11401 gtcggtatcc atcgggctcg tgttcgtgca gctggtgtcg ggaggttacg ggaagccatc
    11461 ccggcccacc agcgcgccac gatgcaacgc gcgattgcgg ggatgaaatg cagtcggacg
    11521 ggcaccggcg gatcgcgtgc ggcgtgcttg cggtatcggc accgcgtcgt tcgccggcga
    11581 taccgacgaa catggaatag gcaccggccc ggcgctgttc cagtgacaca cgcccatcgt
    11641 cggcgtgcgg cgcctcccgc gtccgctgca acagggggcg ggcgaaatct cgttccacgc
    11701 cgtcttgcgt aacctgccgc gaaggagtct tctcatgacc gcaacttctc ccatcgcgct
    11761 tgctgcgcga caatttgccg tcgtcgcctg cgcactcgcg ctcagtacgc cggttttcgc
    11821 cgacgcgggg aactacgagg tgacgaagct cgtcgccgat ctgcctggcg tcgccgcgaa
    11881 caccgacccc aacctcgtca acgggtgggg catcgcgatg cgtgccacca gcccgatctg
    11941 ggtgtcgaat aacgcgacgg gcacgtcgac gctctacaac ggtctcggcg tgcagatttc
    12001 tccgccttcg ccggtcatca tcccggctgc gaatgcatcc ggccggggtg tgccgacggg
    12061 gatcacgtcg aacccctcca ccagcgactt cctgctgggc ggcccgggaa cggcggccat
    12121 ctttgtctgg gcgaccgagg acggagccat cgccgcatgg aacggcggcc ccgcggcatc
    12181 cgtcaagttc gccgcgaagg acggcgccgt ctacaagggt ctcgcactcg ccgggaacgg
    12241 cagcagcttt cggctgtacg ccgccgattt tcacaacggc aagatcgacg tgctcgacaa
    12301 cacgttcacg ccgacgagcg ttgccggcgg attcgtcgat ccgcgcatcc cggccggttt
    12361 cgcgccattc aacatcatga acctgcaggg caatctctac gttgcctatg cgttgcgcga
    12421 gccgggcggc gatgacgagg ttgccggcgc cggcctcggc ttcgtcgatg tcttcgacgc
    12481 cgacggcttc ctcgtcgcgc gcgcagcgac gcgcggcaag ctgaacgccc cgtgggggat
    12541 ggcgatcgcg ccggccggat tcggcaggtt cagcaaccat ctgctcgtcg gcaacttcgg
    12601 cgatggcacg atcaacgcgt acgacgtcaa gaacttcacg ttcgccgggc agctccatgc
    12661 gtcgaacggc cgcacgctgg tgatcgacgg cctgtggggc atcgcgttcg gcaacggctt
    12721 ccagcgccag ccgaccgatg cgctgttctt cgccgccggc cctgacgacg aaacccacgg
    12781 cctgtacggc cgcatcgatc cggcgccgta atactgcggc gcggggcgcg cgtcctccgg
    12841 cgtgccccgc gaacggccgg tcgttacgcc gcttccgcct cgcgcgactt gttctgcgcc
    12901 cgcagcgctg cgagctcgag gtcctcgagc tcgcgctttt tctgcatctg gagcttcttc
    12961 tcggcgtgca gcgccacgag ccgttcctgc tcgagctggc gctttttctc ctgctgcttc
    13021 acgagctcct gctgccgctg ctgttccagc gtttctccgc ttggcgcctc gtcgatcgaa
    13081 agcgaaagtc cgccggcggt cgcaggctcc cctcgctcgc tccgcagctt gatgttgagc
    13141 cgcagctcat tggccgaatc ggcgttgcgg atcgcttcct cgtagccgat gtggccgtcg
    13201 ttgtagagct cgaacagcgc ccagtcgaac gtgcgcatgc cgagctcgcg cgacttcgcc
    13261 atgatcgcct tcagctcgtg gaattcgccc ttgaagatcc ggtcggcaat ggtcggcgtg
    13321 ttgatcagga tctcgatggc ggccttgcgg ccttcgccgt cctgggtgcg caccagccgt
    13381 tgcgaaacga tggcgcgcag gttcgccgac aggtccatca gcagctgatt gcgccgctcc
    13441 tcggggaaga agttcacgat ccggtcgagc gtctggtttg cgttgttcgc gtgcagcgtg
    13501 ccgaggcaaa gatggccggt ctcggcgaag gtgatcgcat gctccatcgt ctcggcatcg
    13561 cggatttcgc cgatgaggat cacgtcgggc gcctggcgca gcgtgttctt cagcgcaccg
    13621 tgccaggaat gggtgtcgac gccgacttcg cggtgcgtca ccagcgattg cttcgacgcg
    13681 tgcacgtatt cgaccgggtc ctcgatggtg atgatgtggc cggacgacgt cgagttgcga
    13741 tggtcgatca tcgcggcaag cgacgtcgat ttcccggagc ccgtggcgcc gacgaccagc
    13801 acgagcccgc gcttgtgcat cacgatgtcc ttgagcgtct cgggcagccc cagcttctcg
    13861 aacgtcggca tttccgaggc gatcgtgcgg atcaccatcc cgacctgctg ctgctgcacg
    13921 aggatgttga cccgaaagcg cgaaagcccg ggaaccgaga tcgcgaaatt gcactcgagg
    13981 tcgcgcgcga actcctcgcg ctgccgttcg ttcatcagcg catgcgcaag catccgcgtc
    14041 atctgtcccg acatcttctg cgcggtgagc ggctgcatct gcccgtgcgc cttcatgctc
    14101 gggggaaagt cggcggcgat gaacaggtcg gacccgccgg caccctgcat cgccgcgagc
    14161 agcttgtgca tgtgggcgcg tgcttgttct tcacttacgt tggacatgga gattcctgtg
    14221 gcacacagat ggttgtatcg ggaggaccgc ggatatcggc gacggtgctc gcgcacgcgt
    14281 cgcgtccgcg gttgccatgc aggaatcagg ccacgacgcg agctaaccgg acgccgggat
    14341 gcgcgatgcg tgctcgagcg gaatcgcctg cgcgtcggtc accgactgta cggcgcggcg
    14401 aaattcgagc ggcgcctgga tgtcggagaa cgtctccccg gtgccgccgc tgccggtaac
    14461 gacgatgtgg ccatagccaa gcatgcgccc ggcgagcgtc tggttgacgg cgattgcctc
    14521 gatcttgttc agcagcaact cgaccgagcg cgtattgaag atgccggtct tcatcatcac
    14581 gcgcttgttg gtgaccgcaa aatcggagct catccgtttg acgattgcgg ccaggagcac
    14641 gatgagcccg accagcggcg caatcagcac ccaatcggtc catgtgcgct gcaggtaaag
    14701 ccaggcaggg gggacgagcg tcgccagcag gaacaccccg gggccgacga gcagcttcca
    14761 gtgcagccgc gtgcgaaaga cgacgtgctc gccctccagc aggttgccgt tgatgtagct
    14821 caccggggcg cggggttctg cggcacgtcg ctcgggcgcg ttcgcgcggc gccggtatcg
    14881 gcggtcggtc cgctcgccgg cgcgcgcgcc gctttcggtt cctgcgcttt tttggagggc
    14941 gtgcggtaag catgatcggg aaagatcgtt ccctgctcgc ccaggttgtg ccccccgtgg
    15001 aaataggcgt acgaatcgcc ggtgaacgga ttcatgctgc tgtcgccgtt gtcgccgccg
    15061 ccgccgccgc cgccgcggtt ggccgatgcg ccgcccgata tcccgaacgc cagcaaggcc
    15121 gccgccaatg agcgagcaat acgtaatcgc gcatttcgat ccattccggt ctcctcgatt
    15181 ggatgtcgac gggccgcttc gccgcatcga tcatgattag aacgcgagga ttcgacgctg
    15241 ttcagtctgg ggcgcgttaa cgggccttga tcaatcgacg cacgacaacg ggcgcgcaac
    15301 gtcctcgagc ggcttgcgct cggcggcgat gccgaggcgc caggcgaacg ccgcggcgat
    15361 cagcatgagg agcgcgccga gcaggtagcc cgatgcaatc gccagcggct ggccggtgcc
    15421 gatcagcacg ccgaacagcc acggcgcacc gatcccgccg atccccgtgc cgagcgcata
    15481 gaagaacgcg atcgacagcg cgcgcacctc gagcggaaag ctttcgctga cgacgagata
    15541 ggcggagctc gcggccgccg acgcgaagaa gaagacgagg gtccacgcaa gcgtctgcgt
    15601 ggctgcgctc agcagtcccg cggcgaacag caccgcggtc agtccgagca gcaccgcggc
    15661 gagcgcatac gtcgtcgcga tcatctggcg ccggccccac gtatcgaagt agcgcccgag
    15721 cacgagcggc ccggcgaagt tgccgatcgc gaacggcagc agatagagcc cgatgtcctc
    15781 gggcggcacc gcataaaaac gcccgagcac cagcgcgtac gtgaagaaga tcgcgttgta
    15841 gcagaacgcc tgcgccgtca tcagcacgag gccgagcagc gcgcggcgcg gatagtgcgc
    15901 aaagagcgtc attccgaggt cgacgaaatt cgcgctgcgc gcgcgaatgc gaagacgcaa
    15961 cgaccgcagc gacgtcgcgg gatgggcgga ggcgcacatg cgctcgacgt cggcggcgat
    16021 gcgttccgcc tccgcttcgc gtccgtgcag caacagccac cgcggacttt cgggcagatg
    16081 gcgccgcagc gcgagaatgg cgagaccgag cagcgcgccc aagccgaagc agacgcgcca
    16141 gccgacgtcg agcggcaggc gtccgggtgc gagaagcagg ctggaaccga tcgcgccgag
    16201 cacggcgccc agccagaagc tgccgttgac gccgaggtcg gtgcggccgc ggtagcgggc
    16261 cggaatcagt tcctggatcg ccgaattgat cgccgagtac tcgccaccga tcccggcgcc
    16321 ggtgatgaag cggcacagcg cgaacgtcgc gaaatcggtc gacagggcgg tcgcgcccgt
    16381 cccaagcacg tacacgccga gcgtcacgaa gaagagacgc ttgcgtccga agcggtcggt
    16441 cagccagccg aacacaaggg cgccgagcac ggcgcccgcg aggtacgcgc tcgccgacag
    16501 cccgacctgc gcgtcctgga agtgcagtgt cgggctcgcc ttcagcgcgc cggacaccgc
    16561 gcccgcgagc gtgacctcga gcccgtccag cacccacgtg atgccgagcg cggcgatgac
    16621 gagcgtgtgg aagcgcgacc agcgcagcgc gtcgaggcgc gccgggaccg cgctttcgac
    16681 gatgcgatcg tcggtcagtg ccgcgtaccg cctttgccgg gcttctttcc ccgggctgtc
    16741 accgcttcga ccgacaatgc gaacgtgcac tgctcgttcg catcgagcgc gccgacgccg
    16801 atcagcttgc gcgcgatttc ttcaccgcgg ccattgcgga tcgtcacgac gaccgtgtat
    16861 tcccagggat cggcatctgc ggagtggcga atggcgcctt cgatgcgcag cggcgtgatc
    16921 gcgggcaccg gcgcggcatc gccgccggcg tcgaagcgca ggtagtgccg gcaggccgga
    16981 cacacgcccg agct
//
LOCUS       DASSVX010000071        12967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_619210, whole genome shotgun sequence.
ACCESSION   DASSVX010000071 DASSVX010000000
VERSION     DASSVX010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12967
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619210"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..643
                     /locus_tag="VFO53_03315"
     CDS             245..643
                     /locus_tag="VFO53_03315"
                     /inference="COORDINATES: protein motif:HMM:NF036460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5329 domain-containing protein"
                     /protein_id="HET8875335.1"
                     /translation="MTVHRIRRPLAATVAALAFAITHAAAAATGAPATDEIDHLLHYV
                     AASSCTFVRNGAEYPADKASEHLASKYRFVGARISTAEDFIKYLATQSSMSGEPYHVK
                     CGKTDALSGAWLAAELDRYRKKPRLQQVSR"
     gene            complement(672..1682)
                     /gene="galE"
                     /locus_tag="VFO53_03320"
     CDS             complement(672..1682)
                     /gene="galE"
                     /locus_tag="VFO53_03320"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET8875336.1"
                     /translation="MPDSVLITGGAGFVGSHVVVELARSGYAPVVLDNFTNSTPAIRT
                     RLEALAGRPVPCVTADVRDVAALRSAFHDHPITAVVHCAGLKNVAESQARPLAYYDVN
                     VGGTLALVEVMGEAGVGTLVFSSAASVYGDADDLCLSEDAPPEPASVYGRTKRVVEDF
                     LRDLSRANANWRIAIVRKFNVAGAHSSAMMGEAPRAGSSHLIPLLCRVAAGEASEIIV
                     HGDDWDTADGTGVRDYVHVQDVADGFLRALRFVASKPGLLTVNLGSGQPHSVLGVLAA
                     FERACGRSIARLTGPRRPGDVAGSCADITRAAELIGWRPTRDLDAICADAWRWQKNGG
                     RY"
     gene            complement(1834..2223)
                     /gene="rplQ"
                     /locus_tag="VFO53_03325"
     CDS             complement(1834..2223)
                     /gene="rplQ"
                     /locus_tag="VFO53_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET8875337.1"
                     /translation="MRHRHGLRKLNRTSAHRLAMLRNMTNSLFTHEAIRTTLPKAKEL
                     RRVAEPLITLGKTPTLANRRLAFDRLRDRDVVTKLFNDLGPRFKARPGGYLRILKFGF
                     RPGDAAPMALIELVDRPESPVDEAEAA"
     gene            complement(2313..3290)
                     /gene="rpoA"
                     /locus_tag="VFO53_03330"
     CDS             complement(2313..3290)
                     /gene="rpoA"
                     /locus_tag="VFO53_03330"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET8875338.1"
                     /translation="MQSNVLLKPRIIDVQSMSPFHAKVVMEPFERGYGHTLGNALRRV
                     LLSSMPGYAPTEVKITGVLHEYSALDGVQEDVVDVLLNLKGVVLKLHNRDSVMLKLAK
                     TGEGPVTAADIEPNHDVEIINPEHLIAHLTAGGKIEMEIKVERGRGYQPATARTKPEG
                     GRTIGSILLDASFSPVRRVSYAVESARVEQRTDLDKLVLDIETNGVVEPEQAVRYAAS
                     VLVEQLSVFADLKGTDQPSAERASPQVDPVLLRPVDDLELTVRSANCLKAENIYYIGD
                     LIQRSETELLKTPNLGRKSLNEIKEVLASRGLSLGMRLENWPPAGLDRP"
     gene            complement(3351..3974)
                     /gene="rpsD"
                     /locus_tag="VFO53_03335"
     CDS             complement(3351..3974)
                     /gene="rpsD"
                     /locus_tag="VFO53_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET8875339.1"
                     /translation="MARYTGPKCKLARREGTDLFLKSARRSLDSKCKLDTKPGQHGVR
                     SGARTSDYGHQLREKQKLRRIYGVLERQFRRYFAEAAKGTSSTGTNLLQLLESRLDNV
                     VYRMGLGSTRAEARQLVSHRSITVNGRVLNVPSALLRSGDVVSTTERAKSQLRIQDAL
                     QLAEKVGFPSWVEVDPKKMTGTFKGAPDRSEFGQDINESLVVELYSK"
     gene            complement(4070..4477)
                     /gene="rpsK"
                     /locus_tag="VFO53_03340"
     CDS             complement(4070..4477)
                     /gene="rpsK"
                     /locus_tag="VFO53_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET8875340.1"
                     /translation="MAQQPSQKGAAARTRKKVKKSVSEGIAHIHASFNNTIITITDRQ
                     GNALSWATSGGAGFKGSRKSTPFAAQVAAESAGRAAQECGVKNLEVRIKGPGPGRESA
                     VRALNALGLKISSISDVTPVPHNGCRPPKRRRI"
     gene            complement(4508..4888)
                     /gene="rpsM"
                     /locus_tag="VFO53_03345"
     CDS             complement(4508..4888)
                     /gene="rpsM"
                     /locus_tag="VFO53_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET8875341.1"
                     /translation="MARIAGVNIPNHQHAEIALTAIYGIGRPRAKAICVAAGVPPGVK
                     IKDLTDAQMDKLRDNIGKFTVEGDLRREVTMSIKRLMDLGCYRGVRHRKGLPVRGQRT
                     RTNARTRKGPKKGRTVALSKGVTK"
     gene            complement(5090..5206)
                     /gene="rpmJ"
                     /locus_tag="VFO53_03350"
     CDS             complement(5090..5206)
                     /gene="rpmJ"
                     /locus_tag="VFO53_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET8875342.1"
                     /translation="MKVLASVKKICRNCKIVRRHGVVRVICTADARHKQRQG"
     gene            complement(5301..5519)
                     /gene="infA"
                     /locus_tag="VFO53_03355"
     CDS             complement(5301..5519)
                     /gene="infA"
                     /locus_tag="VFO53_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET8875343.1"
                     /translation="MAKEETIAMQGEVAEMLPNATFRVKLENGHVVLAHISGKMRMHY
                     IKILPGDKVTVELTPYDLSRARITFRAR"
     gene            complement(5593..6915)
                     /gene="secY"
                     /locus_tag="VFO53_03360"
     CDS             complement(5593..6915)
                     /gene="secY"
                     /locus_tag="VFO53_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET8875344.1"
                     /translation="MALANPARKSGTKYGDLKKRLWFLLGAMVVYRVGTHIPVPGINA
                     SVLDELFRGQQGGILGLLNVFSGGALSRFSIFALGIMPYISASIIMQLCTTIVPSLEA
                     LKKEGQSGQRKITQYTRYATLGLSFFQAFAISAALEAQPGLVVDPGLAFRMVSAVTLV
                     TGTMFLMWLGEQITERGLGNGISLIIFAGIAAGLPRAIGQTLEQGRTGAYSWILIIGI
                     AALVVLVTAFVVFMERALRKILVNYAKRQVGNKVYQGQSSHLPLKINMAGVIPPIFAS
                     SIILFPATLTQWAGTSESTIWIRDLAATLAPGQPVHEILYAAAIIFFCFFYTALQYNP
                     KDTADNLKKSGAFIPGYRPGEQTAKYLEKITLRLTLVGSLYLVVVCFVPNFLIAWKNV
                     PFYFGGTSLLIIVVVTMDFMTQVQAYLMSQQYESLLRKANFKGGVPTR"
     gene            complement(6951..7382)
                     /gene="rplO"
                     /locus_tag="VFO53_03365"
     CDS             complement(6951..7382)
                     /gene="rplO"
                     /locus_tag="VFO53_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET8875345.1"
                     /translation="MRLNSMKPSAGSNKARRRVGRGIGSGLGKTAGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFVSRSRNDIAEVRLSDLARLPVADIDLLALKAAGLVP
                     AIAKSAKVIKTGKLERAVKLTGVAATQGAKAAIEAAGGSVA"
     gene            complement(7399..7587)
                     /gene="rpmD"
                     /locus_tag="VFO53_03370"
     CDS             complement(7399..7587)
                     /gene="rpmD"
                     /locus_tag="VFO53_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET8875346.1"
                     /translation="MAGAKTFKVTLVKGTNSCRKDHRATVRGLGLKRIRQTVELEDTP
                     AVRGMVNKVSYLVSVAGE"
     gene            complement(7590..8123)
                     /gene="rpsE"
                     /locus_tag="VFO53_03375"
     CDS             complement(7590..8123)
                     /gene="rpsE"
                     /locus_tag="VFO53_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET8875347.1"
                     /translation="MAQQRSNPRQPQQQDDRGDGLKEKMISINRVTKVVKGGRILGFA
                     ALTVVGDGDGRIGMGKGKAKEVPVAVQKAMEQARRKMVKVRLKKGTLHHAVEGRHGST
                     RVHMQPASEGTGVIAGGAMRAVFDVVGVTNILAKCHGPTNPYNVVRATINALEAINAP
                     ADIAAKRGKTVEEILEA"
     gene            complement(8140..8493)
                     /gene="rplR"
                     /locus_tag="VFO53_03380"
     CDS             complement(8140..8493)
                     /gene="rplR"
                     /locus_tag="VFO53_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET8875348.1"
                     /translation="MNKKDARVRRARKTRVRIAEQRATRLTVNRSNSHIYAQIIAPTG
                     DKVLASASTLEADVRKDVKNGGNKAAATLVGRRIAERAKALGIEAVAFDRAGFRFHGR
                     VKALADAAREGGLKF"
     gene            complement(8508..9035)
                     /gene="rplF"
                     /locus_tag="VFO53_03385"
     CDS             complement(8508..9035)
                     /gene="rplF"
                     /locus_tag="VFO53_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET8875349.1"
                     /translation="MSRIGNNPVPVPPKVEVTLAAGEISVKGPLGTLTRQYGPSVSVE
                     RSGETLVFKAADDEARAMQGTLRALVANMVKGVTQGFEKRLALVGVGYRAQAAGDRIN
                     LSLGFSHPVVHAMPKGVKVETPQQTEIVVKGIDKQQVGQVAAEIRAYRPPEPYKGKGV
                     RYADERVKIKETKKK"
     gene            complement(9049..9447)
                     /gene="rpsH"
                     /locus_tag="VFO53_03390"
     CDS             complement(9049..9447)
                     /gene="rpsH"
                     /locus_tag="VFO53_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET8875350.1"
                     /translation="MSMTDPIADMLTRIRNAQMTERAIVAMPASKVKVAIARVLKDEG
                     YIDGFKISGEETRKPLLEIALRYYAGKPVIEKIERISKPGLRIYKSKDDIPRVMNGLG
                     IAIVSTSHGVMTDRKARATGVGGEVLCIVA"
     gene            complement(9463..9768)
                     /gene="rpsN"
                     /locus_tag="VFO53_03395"
     CDS             complement(9463..9768)
                     /gene="rpsN"
                     /locus_tag="VFO53_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET8875351.1"
                     /translation="MSKLALINRQHKREKLVAKYAKKRATLESIIDSPRMSDEDRYAA
                     RLKLQQLPRNANPTRLRNRCTITGRPRGVYSKFGLGRNKLREYAMRGEIPGIVKASW"
     gene            complement(9780..10316)
                     /gene="rplE"
                     /locus_tag="VFO53_03400"
                     /pseudo
     CDS             complement(9780..10316)
                     /gene="rplE"
                     /locus_tag="VFO53_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139711.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
     gene            complement(10453..10770)
                     /gene="rplX"
                     /locus_tag="VFO53_03405"
     CDS             complement(10453..10770)
                     /gene="rplX"
                     /locus_tag="VFO53_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET8875352.1"
                     /translation="MRKIRKGDSVVVIAGRDKGKRGDVASYVDDTHVIVNGVNQVKRH
                     TKPNPMKNQPGGIVTKEVPIHISNVAIWNPVTRKPDRIGFRLMQDGRKLRFFKSNGEQ
                     ING"
     gene            complement(10797..11165)
                     /gene="rplN"
                     /locus_tag="VFO53_03410"
     CDS             complement(10797..11165)
                     /gene="rplN"
                     /locus_tag="VFO53_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET8875353.1"
                     /translation="MIQMQTVLDVADNTGARAVMCIKVLGGSKRRYAHIGDVIKVSVK
                     EAAPRGRVKKGEIYNAVVVRTRQGVRRDDGALVRFDTNAAVLLNNKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            complement(11398..11700)
                     /gene="rpsQ"
                     /locus_tag="VFO53_03415"
     CDS             complement(11398..11700)
                     /gene="rpsQ"
                     /locus_tag="VFO53_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET8875354.1"
                     /translation="MNEQAAISTAEAPAGRKALVATVTGRVVSDKMNKTVTVLVERRV
                     KHPIYDKVVIKSNKYHAHDEANECKAGDLVEIRASRKLSKTKAWRVLRVVEKAKIV"
     gene            complement(11715..11957)
                     /gene="rpmC"
                     /locus_tag="VFO53_03420"
     CDS             complement(11715..11957)
                     /gene="rpmC"
                     /locus_tag="VFO53_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET8875355.1"
                     /translation="MKLNETRRTLGGKNAAELTTELEALLKEQFGLRMQRATQQLTNT
                     SKLKDVRRGIARVRTLLAQRNGGTAASAPQAKGKRT"
     gene            complement(11971..12387)
                     /gene="rplP"
                     /locus_tag="VFO53_03425"
     CDS             complement(11971..12387)
                     /gene="rplP"
                     /locus_tag="VFO53_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET8875356.1"
                     /translation="MLQPARRKYRKEQKGRNKGVATVGNKVSFGEYGLKAIVRGRLTS
                     RQIEAARRAMTRHIKRGGRIWIRIFPDKPVSKKPAEVRMGNGKGNPEYYVAEIVPGKV
                     LYEMDGVEEALAREAFALAAAKLPLKTIFVHRLVGG"
     gene            complement(12371..>12967)
                     /gene="rpsC"
                     /locus_tag="VFO53_03430"
     CDS             complement(12371..>12967)
                     /gene="rpsC"
                     /locus_tag="VFO53_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET8875357.1"
                     /translation="ASVAKVTIERPAKNARITIHSARPGVVIGKKGEDIETLRGDLRK
                     MMGSDVGLNIEEIRKPEIDAQLVADSIAQQLEKRIMFRRAMKRAMQNAMRLGAQGIKI
                     MSSGRLNGVEIARREWYREGRVPLHTLRADIDYGFAEAHTTYGVIGIKVWVFKGEVMA
                     HNAEPAQAPAPAPETSKRPRRSGPGPAAGAGAKNAATS"
BASE COUNT         1909 a         4370 c         4159 g         2529 t
ORIGIN      
        1 cgtcgtgatg cgaccgtcgt cgccgacgcc gatcgcgacg tcgctcttcc agccgtcgtc
       61 gagcagtgcg gcgggtgcga tcagcgtctt catccgagcc gtgggtcgtt catcgttgat
      121 caggcgctaa atcgggggcg cgaccgcatg cgccgattgt aaccgcgcga agcgacgcca
      181 catgggaacc gcgcggcgac gatcctgtcg gacacgcgct accctttttg cccgagacac
      241 accgatgacc gtgcatcgca tccgccgtcc tctcgctgcg accgtcgccg cgctcgcctt
      301 cgcgattaca cacgctgccg cggcagccac cggcgcaccg gccacggacg agatcgacca
      361 cctgctgcac tatgtggccg cgtcgtcgtg cacgttcgtg cgcaacggcg ccgaatatcc
      421 ggccgacaag gcgagcgagc acctcgcgag caagtatcga ttcgtcggcg cccgcatctc
      481 gaccgccgag gatttcatca agtacctggc gacgcaaagc agcatgtcgg gcgagcccta
      541 ccacgtgaaa tgcggcaaga ccgatgcgct gtcgggcgca tggctcgccg ccgagctcga
      601 tcgctaccgc aagaagccgc gcctgcagca ggtgtcgcgg taatcccgcg cagcagcctt
      661 tacatcgagg gtcagtagcg gccgccgttc ttttgccacc tccatgcatc ggcgcagatg
      721 gcatcgagat cgcgtgtcgg gcgccaaccg atcagttcgg ccgcgcgcgt gatgtcggcg
      781 cagcttccgg cgacgtcgcc ggggcgccgc gggcccgtca ggcgcgcaat cgagcggccg
      841 cacgcgcgct cgaacgcggc cagcacgccg agcaccgaat gcggctgacc gctgcccagg
      901 ttgacggtga gcagccccgg cttcgaggcg acaaagcgga gcgcgcgcag gaagccatcg
      961 gccacgtcct gcacgtgcac gtaatcgcgc acgccggtgc catcggccgt atcccagtcg
     1021 tcgccatgca cgatgatctc gctggcctcg cccgccgcga cgcggcagag cagcggtatc
     1081 aggtggctcg acccggcgcg cggcgcttcg cccatcatcg ccgacgaatg cgcgccggcc
     1141 acgttgaact tgcgtacgat cgcgatccgc cagttcgcgt tggcgcgcga caggtcgcgc
     1201 aggaagtcct cgaccacgcg tttggtgcgt ccgtagacgc tcgccggttc gggcggcgca
     1261 tcctcggaca ggcaaaggtc gtcggcgtcg ccatacacgc tcgccgcaga gctgaacacc
     1321 agcgtgccca cgcccgcctc gcccatcacc tcgacgagcg ccagcgtgcc gccgacgttg
     1381 acgtcgtagt acgcgagggg ccgtgcctgc gattcggcga cgttcttgag acccgcgcaa
     1441 tgaacgaccg ccgtgatcgg gtgatcgtgg aaggcgctgc gcagtgccgc gacatcgcga
     1501 acgtcggcgg tgacgcaggg cacgggccgg ccggcaagtg cctcgagccg cgtgcggatc
     1561 gccggcgtgc tgttggtgaa gttgtcgagc acgaccggcg cgtagccgct tcgcgcgagc
     1621 tccacgacga cgtgcgagcc gacgaagccg gcgcctccgg tgatcaggac gctgtcgggc
     1681 atgacgccga tgctacacga tcgtaaacgc gggctcggag acgacgttcg cgctcgtgcg
     1741 cacgaggtcg gcgggaaagt cgtccctgac cctgcataca acgcgaaaag gccgggcgat
     1801 gcccggcctt ttcgctgcag cgaatcatgc gatctacgcg gcctcggcct cgtcgaccgg
     1861 cgattcggga cgatcgacga gctcgatcag cgccatcggt gccgcatcac cggggcggaa
     1921 gccgaacttc aggatgcgca ggtaaccgcc cggccgcgcc ttgaagcgtg ggccgaggtc
     1981 gttgaagagc ttggtgacca cgtcgcgatc gcgcaggcgg tcgaacgcga gccggcgatt
     2041 ggcaagggtg ggcgtcttgc cgagcgtgat cagcggctcg gccacgcggc gcagctcctt
     2101 cgccttcggc agcgtcgtgc ggatcgcttc gtgcgtgaag agcgagttgg tcatgttgcg
     2161 cagcatcgcg aggcgatgcg cgctcgtccg gttcaacttc cgcaatccgt gtcggtgtct
     2221 catgtctttt cctggcaatt gccgctgaaa atcgtcgggc tccgcccgcg attttcagga
     2281 agccctatcc gtaaaaagta gggggttact ttttacggtc tatccagtcc cgccgggggc
     2341 cagttttcca gccgcatgcc gagcgaaagg ccgcgcgatg cgagcacttc cttgatctcg
     2401 ttcagcgact tgcgcccgag gttgggcgtc ttcagaagct cggtttcgga gcgctggatc
     2461 aggtcgccga tgtagtagat gttctcggcc ttgaggcagt tggccgagcg caccgtcagc
     2521 tcgagatcgt cgaccgggcg cagcagcacc gggtcgactt gcggcgacgc acgctccgcc
     2581 gacggctggt cggtgccctt gaggtcggcg aacaccgaca actgctcgac gagcacgctc
     2641 gcggcgtagc gaaccgcctg ctcgggctcg acgacgccgt tggtctcgat gtcgaggacg
     2701 agcttgtcga ggtcggtgcg ctgctcgacg cgcgccgatt cgacggcgta gctcacgcga
     2761 cgcaccggcg agaacgacgc gtcgagcagg atgctgccga tggtgcggcc gccctcgggc
     2821 ttcgtgcgcg ccgtcgcggg ttgatagccg cggccgcgct cgaccttgat ctccatctcg
     2881 atcttgccgc ccgccgtcag gtgcgcgatc aggtgctctg gattgatgat ctcgacgtcg
     2941 tggttcggct cgatatcggc ggccgtgacg ggcccttcac cggtcttcgc gagcttgagc
     3001 atcacgctgt cgcggttgtg cagcttcagc acgacgccct tcaggttgag gagcacgtcg
     3061 acgacgtcct cctgcacgcc gtcgagcgcc gagtactcgt gcagcacgcc ggtgatcttc
     3121 acctcggtgg gcgcgtatcc gggcatcgac gacagcagca cgcgccgcag cgcgttgcca
     3181 agcgtatgcc cgtacccgcg ctcgaacggc tccatcacca ccttggcgtg gaacggcgac
     3241 atgctttgca cgtcgatgat gcggggcttc aacagaacat tgctctgcat gcggtacctt
     3301 taaggatgga atcgacgccg agcgcctgac ggcgccggcg tcgaacggcg ttacttcgag
     3361 tagagttcta cgacgaggct ttcgttgatg tcctgtccga actcggaacg gtcgggcgca
     3421 cccttgaacg ttccggtcat cttcttcggg tcgacctcga cccacgaggg gaagccgacc
     3481 ttctcggcga gctggagcgc gtcctggatg cgcagctgcg atttcgcgcg ctcggtcgtc
     3541 gacacgacgt cgccgctcct gagcagcgcc gacggcacgt tgaggacgcg tccattcacg
     3601 gtgatcgagc ggtgcgacac gagctggcgc gcttccgcgc gcgtcgaccc gagccccatc
     3661 cggtaaacga cgttgtcgag ccgcgactcg aggagctgca gcaggttggt gccggtcgag
     3721 ctcgtaccct tcgccgcttc ggcgaagtag cggcggaact ggcgctcgag cacgccgtag
     3781 atgcggcgca gcttctgctt ttcacgcagc tggtggccgt agtcggacgt ccgcgcgccg
     3841 gagcgcacgc cgtgctggcc gggcttcgtg tccagcttgc acttggaatc gagcgaacgg
     3901 cgcgcgctct tcaggaacag gtcggttcct tcccgccgcg cgagcttgca tttgggtccc
     3961 gtatatcgtg ccactgtttt tcctcgtctt tactgccgag aatcgtcggg catcgcccgc
     4021 gatcgtcagg tattcgaatc ggacgaaagt aggggtgtac ttttgtccgt catattcttc
     4081 gccgcttcgg cgggcggcac ccgttgtgcg gcaccggcgt cacgtcggag atgctgctga
     4141 tcttgaggcc cagcgcgttc aacgcgcgca ccgccgattc gcggccggga cccggtcctt
     4201 tgatgcgcac ctcgaggttc ttgacgccgc actcctgcgc tgcgcgcccc gccgattcgg
     4261 cggcgacctg cgccgcgaac ggcgtcgact tgcgcgagcc cttgaagccc gcaccgcccg
     4321 acgtcgccca cgacagcgca ttgccctgcc ggtcggtgat cgtgatgatc gtgttgttga
     4381 aggacgcgtg gatgtgcgcg atgccttcgg acacgctctt cttgaccttc ttgcgcgtcc
     4441 ttgcggcggc gcccttttgc gacggttgct gtgccatctt cgtgtgatcc ttgcctttct
     4501 cgcttcgtta cttggtgacg cccttcgaca gcgccacggt gcggcccttc ttcggtccct
     4561 tgcgcgtgcg cgcgttggta cgcgtgcgct ggccgcgaac cggcagcccc ttgcgatggc
     4621 gcacgccgcg atagcagccg agatccatca gccgcttgat gctcatcgtc acctcgcggc
     4681 gaagatcgcc ctcgacggtg aacttgccga tgttgtcgcg cagcttgtcc atctgcgcgt
     4741 cggtgagatc cttgatcttc acgcccgggg gcacgccggc cgcgacgcag atcgccttgg
     4801 cgcgcgggcg gccgatgccg tagatcgcgg tgagcgcgat ttcggcgtgc tgatgattcg
     4861 gaatgtttac gcctgcaata cgggccatgg tttacctgtc gtcaattgtt tcgtcgccgc
     4921 ctgggcgtca gtcgggaagg gccgccccga ccgactgacc cgccccccgg ggggcagcgc
     4981 gcggagcgag cgtgggggga tgttcatccc agccgggaag ggccgccccg cccgactgac
     5041 ccgccccccg ggggcagcgc gcggagtgag cgtgggggga tgttccatcc tagccctgcc
     5101 gctgcttgtg ccgcgcgtcg gccgtgcaga tcacgcgcac gacgccgtgc cgccgcacga
     5161 tcttgcagtt gcggcagatt ttcttcaccg atgccaatac cttcatctcg acctcgcgga
     5221 aaacattcgt cgcggctcgc gccgcggcac cggtgacacc ggcgcatccg atacctgtta
     5281 cctgtcccct gatacctgaa tcatctcgcc ctgaacgtga tccttgcccg cgaaagatcg
     5341 tacggcgtca attccaccgt caccttgtcg cccggcagga tcttgatgta gtgcatgcgc
     5401 atctttccgg agatgtgcgc cagcacgaca tggccgttct ccagcttcac gcgaaacgtg
     5461 gcgttcggca gcatctcggc cacctcgccc tgcatcgcga tcgtttcttc cttcgccatt
     5521 cgtcacgtag tcgctacgcg gctctttccg ttttccctcg cccgcttgcg ggagagggaa
     5581 aaggggtgag ggtcatcgcg tgggcactcc ccccttgaaa ttcgccttgc gcagcaggct
     5641 ttcgtactgc tgcgacatca ggtacgcctg cacctgcgtc atgaagtcca tcgtcaccac
     5701 cacgatgatc agcagcgatg tgccgccgaa atagaacggc acgttcttcc acgcgatcag
     5761 gaagttcgga acgaagcaca cgacgacgag gtacagcgag ccgaccagcg tcaggcgcag
     5821 cgtgatcttc tccaggtact tcgccgtctg ctcgcccgga cggtaccccg ggatgaacgc
     5881 gccgctcttc ttcaggttgt cggccgtgtc cttcgggttg tactgcagcg cggtgtagaa
     5941 aaagcagaag aagatgatcg ccgccgcgta caggatttca tgcacgggct ggcccggcgc
     6001 cagcgttgcc gcgaggtcgc ggatccagat cgtgctctcg ctggtgcctg cccattgcgt
     6061 cagcgtggcc gggaacagga tgatcgacga ggcgaagatg ggcggaatga cgccggccat
     6121 gttgatcttc agcggcaggt gcgagctctg tccctggtac accttgttgc cgacctggcg
     6181 cttcgcataa ttcacgagga tcttgcgcag cgcccgctcc atgaacacga cgaacgcggt
     6241 gacgagcacc acgagcgccg caatgccgat gatcaggatc cacgagtagg cgcccgtgcg
     6301 gccctgctcg agcgtctgtc cgatggcgcg cggcagcccc gccgcgatgc ccgcgaaaat
     6361 gatcagcgaa atgccgttgc cgaggccacg ctcggtgatc tgctcgccga gccacatcag
     6421 gaacatcgtc ccggtgacga gcgtgaccgc cgacaccatc cggaatgcga gccccggatc
     6481 gacgacgagt cccggctgcg cctcgagcgc ggcggagatc gcgaaggcct ggaagaacga
     6541 cagcccgagc gtcgcgtaac gcgtgtactg cgtgatcttg cgctggcccg actggccttc
     6601 cttcttcaac gcctcgagcg acggcacgat cgtcgtgcaa agctgcatga tgatcgacgc
     6661 cgagatgtac ggcatgatcc cgagcgcgaa gatcgagaag cgcgacagcg cgccgcccga
     6721 aaagacgttg agcaggccga ggattccgcc ctgctgcccg cggaacagct cgtcgagtac
     6781 cgacgcgttg atgcccggca ccgggatatg ggtgccgaca cgataaacga ccatcgcgcc
     6841 cagcaggaac cagaggcgct tcttcaggtc gccgtacttc gtgccgcttt tccgggccgg
     6901 atttgcgagt gccaattgat tgtcctagtt tgcgtcgacc gttcgccgat tcaagcgacg
     6961 ctgccgccgg ccgcttcgat cgcggccttc gcaccttgtg ttgccgcgac gccggtgagc
     7021 ttcaccgcgc gctcgagctt gcccgtcttg atcaccttgg ccgacttcgc gatcgccggg
     7081 acgaggccgg cagccttgag cgcgagcaga tcgatgtcgg cgaccggcag gcgcgccagg
     7141 tcggacagcc gcacctcggc gatgtcgttg cgcgagcgcg acacgaagcc gcgcttgggc
     7201 aggcgccgct gcagcggcat ctggccgccc tcgaagccga ccttgtggaa gccgcccgag
     7261 cgcgacttct ggcccttgtg gccgcgcccg gccgttttgc cgagacccga gccgatgccg
     7321 cggccgacac ggcgccgcgc cttgttgctg ccggcggacg gtttcatcga attgagacgc
     7381 atcgcgtgct ccgaaatttc actcgcccgc taccgacacg aggtagctga ccttgttgac
     7441 catgccgcgc accgccggcg tatcttcgag ctcgacggtc tggcggatgc gcttcaaccc
     7501 cagcccgcgc accgttgcac ggtgatcctt gcgacaggag ttcgtgccct tgacgagcgt
     7561 caccttgaaa gtttttgcgc cggccatcgc tatgcctcga gtatctcttc gacggtcttg
     7621 ccgcgcttcg ccgcgatgtc ggcgggcgcg ttgatcgcct cgagcgcgtt gatcgtcgcg
     7681 cgcacgacgt tgtacgggtt cgtggggccg tgacacttgg cgaggatgtt ggtcacgccg
     7741 acaacgtcga acaccgcgcg catcgcgccg ccggcgatca cgccggtgcc ttccgacgcc
     7801 ggctgcatgt gcacgcgcgt cgagccgtgc cggccctcga ccgcgtggtg cagcgtgccc
     7861 ttcttgaggc gcaccttcac catcttgcgg cgcgcctgct ccatcgcctt ctgcacggcg
     7921 accggcactt ccttcgcctt gcccttgccc atgccgatgc gtccatcgcc gtcaccgacg
     7981 accgtcagcg cggcaaagcc gaggatgcgc ccgcccttca ccaccttggt gacgcgattg
     8041 atcgagatca tcttctcttt caatccgtcg ccgcggtcat cctgctgctg cggctggcgc
     8101 gggttcgaac gttgctgtgc catgaaatgg aatccttgct cagaacttga gaccgccctc
     8161 gcgggcggcg tcggccagcg ccttgacgcg gccatggaag cggaagccgg cgcgatcgaa
     8221 tgcaaccgcc tcgatgccga gcgccttcgc ccgctcggcg atgcgccgtc cgaccagcgt
     8281 cgcggcggcc ttgttgccac cgttcttgac gtccttgcgc acatcggcct cgagcgtcga
     8341 ggcactggca agcaccttgt cgccggtcgg cgcgatgatc tgcgcgtaga tgtgcgagtt
     8401 cgaccggttg acggtgagcc gtgttgcgcg ctgctcggca atgcgcaccc gggttttgcg
     8461 ggcgcggcgg actcgagcgt ctttcttgtt catccgaaat tctccgatta tttcttcttg
     8521 gtctctttga tcttcacgcg ctcgtcggcg tagcgcacgc ccttgccctt gtacggctcc
     8581 ggaggacggt aggcgcggat ctcggcagcg acctgcccga cctgctgctt gtcgatgccc
     8641 ttgacgacga tttcggtctg ctgcggcgtc tcgaccttga cgcccttcgg catcgcatgc
     8701 acgaccggat gcgagaagcc gagtgacagg ttgatcctgt cgccggccgc ctgcgcgcgg
     8761 tacccgacgc cgaccagcgc gagccgcttc tcgaagcctt gcgtgacgcc cttgaccatg
     8821 ttcgcgacga gtgcgcgcag cgtgccctgc attgcgcgtg cctcgtcgtc ggctgccttg
     8881 aatacgagcg tctcgccgct gcgctcgacg ctgaccgacg gaccgtactg gcgcgtgagc
     8941 gtgccgagcg gacccttgac cgagatttcg cccgcggcga gagtcacttc gaccttcggc
     9001 ggcacgggaa ccggattgtt gccgatgcgt gacatgtccc tgttccccct aggcgacgat
     9061 gcagagcact tcgccgccca cgccggtggc gcgcgccttc cgatcggtca tcacgccgtg
     9121 cgacgtcgac acgatggcga tgccgaggcc gttcatcacc cgcgggatgt cgtccttgct
     9181 cttgtagatg cgaagcccgg gcttcgaaat gcgctcgatc ttctcgatca ccggcttgcc
     9241 ggcgtaatag cgcagcgcaa tctcgagcag cggcttcctg gtttcctcgc cggagatctt
     9301 gaatccgtcg atgtagccct cgtccttgag aacgcgggcg atcgcgacct tgaccttgga
     9361 cgccggcatc gccacgatgg cgcgctcggt catctgcgca ttgcggatgc gcgtcagcat
     9421 gtcggcgatg ggatcggtca tgctcatctt gtcacctcgc tcctaccagc tcgccttgac
     9481 gatgcccggg atctcgcccc gcatcgcata ctcgcgcagc ttgttgcggc cgaggccgaa
     9541 tttgctgtac accccacgcg gacggcccgt gatggtgcag cgattacgaa gccgggtcgg
     9601 gttcgcgttg cgcggcagtt gctgcagctt gagccgcgcg gcgtaacggt cctcgtcgga
     9661 catgcgcggg ctgtcgatga tcgactcgag cgtcgcgcgc ttcttcgcgt acttcgcgac
     9721 gagcttctcg cgcttgtgct gccgattgat caatgcgagt ttcgacatgc gggaatcctt
     9781 tagcccctga acggaaaccg gaacgcggcg agcagcgcct tcgcttcggc gtcggtcttc
     9841 gcggtggtcg tgatcgtgat gttcatcccc cggatggcgt cgatcttgtc gtactcgatc
     9901 tcggggaaga tgatctgttc cttgatgccc atgttgtagt tgccgcggcc gtcgaagcca
     9961 cgtcccgaca cgccgcggaa gtcgcgcacg cgcggcagcg cgacggtgac caggcggtcg
    10021 agaaactcgt acatccgttc ctggcgcagc gtgaccatgc agccgatcgg atagccttcg
    10081 cggatcttga agcccgcgat ggccttgcgc gccttcgtga tgaccggctt ctggccgctg
    10141 atcttcgtca tgtcgccgat ggcgttgtcg aggatcttcc ggtcggcagt cgcctcgccg
    10201 acacccatgt tgagcacgat cttcgagatg cgcggcacct gcatcggcga cgcgtagccg
    10261 aactgcttcg tgagcgccgg cgcgatttcc ttgcggtaga cgtccttgag ccgggcgggc
    10321 tgatgcggct ggtcggccgt gtgcgccttt tcggccgcgg ccgcagcctt cgcgggcttc
    10381 tcggccttcg gctgcttcgc gggtgccgcg ggcttggcgg ctttcgcgtc cttcttgtcg
    10441 ggggtcttct tgtcagccat tgatctgctc tccgttcgac ttgaagaagc gcagcttgcg
    10501 gccgtcctgc atcaggcgga agccgatgcg atcgggcttc cgggtgacgg gattccagat
    10561 ggcgacattc gagatgtgga tcggcacttc cttcgtgacg atgccgcccg gctggttctt
    10621 catcgggttc ggcttcgtgt gccgcttcac ctggttgacg ccgttgacga tgacgtgcgt
    10681 gtcgtcgaca tagctcgcga cgtcgccgcg cttgcccttg tcgcgtcccg cgatgacgac
    10741 gacgctgtcg cccttcctga tcttgcgcat ggtggtctct tcgaagtgcg gtggcgtcag
    10801 agaacttccg gcgccagcga gacgatcttc atgaatcgct cggtgcgaag ctcgcgcgtc
    10861 accggcccga aaatgcgggt gccgatcggc tcgagcttgt tgttcagcaa cacggcggca
    10921 ttggtatcga agcggacgag cgcgccgtcg tcacgacgca caccctggcg ggtgcgcacg
    10981 acgaccgcgt tgtagatctc gcccttcttc acgcgtccgc gcggcgcagc ctccttcacg
    11041 gacaccttga tcacgtcgcc gatgtgcgcg taccggcgct tgctgccgcc cagcaccttg
    11101 atgcacatca ccgcgcgcgc gcccgtgttg tcggcgacgt cgagcaccgt ctgcatttga
    11161 atcatgactt cgttctctcc aacttgaacc gcctgccggc gggtcgcgca cgactcgcga
    11221 accggcgaac tgcaaaaagg cggcggccag ttttggaccc cgtcgcggcc tcgtcttcgg
    11281 cgagcccggt tgggatgggc cgcggcgccc ggattgatgc aatgcccgtg gcgacgcgac
    11341 aggggaaacg gcgattatgg caggtatttg cggctactgc aatacccgcg ccaaccgtta
    11401 cacgatcttc gccttctcga ccacgcgcag cacgcgccac gccttcgtct tcgagagctt
    11461 gcgcgacgcc cggatctcga cgagatcacc cgccttgcac tcgttcgctt cgtcgtgcgc
    11521 gtggtacttg ttcgacttga tgacgacctt gtcgtagatc gggtgcttga cccgccgctc
    11581 gacgagcacc gtgacggtct tgttcatctt gtcgctcacg acgcgcccgg tgaccgtcgc
    11641 caccagcgcc ttgcgccccg caggcgcttc cgccgtcgag attgcagcct gctcgttcat
    11701 cggttccccc gcgctcatgt ccgtttacct ttcgcctgcg gcgcggacgc tgccgtgccg
    11761 ccgttccgct gcgcgagaag cgtgcgcacg cgcgcaatgc cgcgccgcac gtccttgagc
    11821 ttcgacgtgt tcgtcagttg ctgtgtcgcg cgctgcatcc gcagcccgaa ctgctccttg
    11881 aggagcgcct cgagctcggt ggtgagctcg gcggcgttct tgccgccgag cgtgcgcctg
    11941 gtttcgttga gtttcatctg tcgcctttcc tcacccaccg acaagccggt gcacgaagat
    12001 cgtcttgagc ggcagcttgg ccgccgcgag cgcgaacgcc tcgcgcgcca gcgcctcctc
    12061 gacgccgtcc atctcgtaga gcaccttgcc cggcacgatc tcggcaacgt agtactcggg
    12121 attgcccttg ccgttgccca tgcgcacttc ggccggcttc ttcgacaccg gcttgtcggg
    12181 gaagatgcgg atccagatgc ggccgccccg cttgatgtgg cgcgtcatcg cccggcgcgc
    12241 ggcttcgatc tgccgcgacg tgaggcgtcc gcggacgatc gccttgaggc cgtactcgcc
    12301 gaagctgacc ttgttgccga cggtcgccac gcccttgttg cggcccttct gttccttccg
    12361 gtatttcctt ctagctggtt gcagcattct tcgctcctgc tcctgcggcc ggtccgggtc
    12421 ccgagcggcg cggacgtttc gacgtttcgg gcgcgggcgc cggcgcctgc gcgggctcgg
    12481 cgttgtgcgc catcacctcg cccttgaaca cccagacctt gatgccgatg acgccgtacg
    12541 tcgtgtgcgc ctcggcgaaa ccgtagtcga tgtcggcgcg cagcgtgtgc agcggcacgc
    12601 ggccctcgcg ataccactcg cggcgcgcga tctcgacgcc gttgaggcgc cccgagctca
    12661 tgatcttgat gccctgcgcg ccgaggcgca tcgcgttctg catcgcgcgc ttcatcgcgc
    12721 ggcggaacat gatccgcttc tcgagctgct gcgcgatcga gtcggcgacg agttgcgcgt
    12781 cgatttccgg cttgcggatt tcctcgatgt tgaggccgac gtcgctgccc atcatcttgc
    12841 gcaggtcgcc gcgcagcgtc tcgatgtcct cgcctttctt gccgatcacc acgccggggc
    12901 gcgcgctgtg gatcgtgatc cgcgcgttct tggcgggacg ctcgatcgtc accttcgcca
    12961 ccgacgc
//
LOCUS       DASSVX010000072         6986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_902598, whole genome shotgun sequence.
ACCESSION   DASSVX010000072 DASSVX010000000
VERSION     DASSVX010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902598"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..5137
                     /locus_tag="VFO53_03435"
     CDS             425..5137
                     /locus_tag="VFO53_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011317200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET8875358.1"
                     /translation="MAPFPIVGIGASAGGLEAFTELLAHLPLDTGMGFVLVQHLDPDH
                     ESALPQLLGRATSLPVEAVTDNTRVAPNRVYVIPPNASLVIVQGVLKLKPRRPKRAPT
                     RTIDTFFESLAEDQRECAIGVVLSGTASDGTVGLEAIKAEGGITFAQDETARYDSMPR
                     SAVNAGCVDFVLAPEAIAQELARIARHPYITGRANAPATPEQDHAEATLHEDDATPLP
                     SGGHGAPPTGARQARNEATRGDAGATAGEQPAANAYKKILLQLRSHSGVDFSLYKSST
                     IQRRVARRIVLSKQSSPESYAHYLRGNTKELDALYTDVLISVTSFFRNPEAFDVLKEK
                     VFPTLLQQRGDEPLRTWVLGCSTGQEAYSIAMAFTEAAEKAPRVRRLQIFATDLNDAL
                     LEKARRGLYAKSLADDIEPDRLRRFFVEEEEGYRIVKAMREMVVFARQNVIADPPFSR
                     MDLVSCRNVLIYLEPDLQKKLLPVFHYALKPRGFLFLGNSESIGSFTDLFEPLGRKSK
                     IYVKKAVATPMLQLGMRRDRGERLPMRPVSTAPADATKAFSGEQSAQREADRITVNQF
                     APPAVLVNADLQILQFRGPTGAFLAPPTGKASFDVLKMAREGLMLPLRTALAKAKKDN
                     RTVRKENVRVDHDGASRTISLEVIPLRNLHERCFLIVFEDTDRTRVAEADAAAHAGTG
                     RGKRRSPASDDARRIAELESTLGDTRDYLQSLQEQHEAANEELQASNEEVQSANEELQ
                     SINEELETSKEELESANEELSTINDEMANRNTELNRANNDLVNLQNSTRLSIVLLGRD
                     LTIRHYSPQAAKQLKLVAADVGRRIGAVRHDLVLTDGDAVSGHRPAGNGVPGTGRNAR
                     KDVDLRGTPAALDLEGFVAEVIDSVRERECHVQDRHGRWYLLRARPYLTLDKHVDGAV
                     LVLTDIDELTRHTQDIAAARDFAEAIIRTVRDPFVILDAGLRVERANDAFYDAFGVAP
                     AETEGRVIFDLGNGQWNVPRLHELLEEILPGKSVFNGFEVTHDFAGIGRRKMLLNARA
                     LQRADGRPARILVGMEDVTEKEAVEEARRAGETHYRMLFAAAPMAIFVCDRDGVIRYY
                     NRRAAELWGREPEIGVERYSGAVKLWLPNGVPLPHEHSPMAGVLATGVSAHNVELEIE
                     RPDGSRLSVLENFSPLKNESGAITGAITSFMDITEQKDATNALQRLSVELLDADRRKN
                     EFLAMLAHELRNPLAPIRTALTLMRRTTKSAGGSPQPAVDMIDRQVGQLVRLVDDLLD
                     VSRISRGQIELQRDEIELASTMHRAVETARPLLDAMEHELTLVLPAEPITVDADAIRL
                     AQIVGNLLNNASKFTDRGGRLRLAIERDGGDIVIRVADNGIGIDKRQLPRVFDLFMQV
                     DTSLTRSATGLGIGLGLVKQLVTLHGGSVEARSGGVGQGSEFIVRLPIVVTAPMRPAA
                     GAGGDSPVNAAAGRILIADDNRDAASSIASLLRLDGNVTETAHDGLAAFTAAEAFRPG
                     VILLDIGMPKMNGYDVARRIRAASWGRDIVLIALTGWGQPEDREKSREAGFDHHLVKP
                     VDPSALTRLLAGIAERSK"
     gene            5134..6261
                     /locus_tag="VFO53_03440"
     CDS             5134..6261
                     /locus_tag="VFO53_03440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HET8875359.1"
                     /translation="MTSSSANDAPAEQLLRLLVEQTTEYAIILLDVDGDVIGWNKGAA
                     HLFGIARDDAIGQPASLIFTAQDIARGTPQQEIAAAEADTAAEDDRWLARSDGSRFWA
                     AGVMIPLHDEQGRLAGFGKILRNRTDLREQIDTLRNQADASDAMVVRKDLFLTTLSHE
                     LRNPLGPLVHATEIIRRKLAAGAGIEDTLGIIDRQVASLVRLVDDLLDLSRVSAGRMA
                     LQKERVTLQDIVDRAVESAGTLVREQRHQLDVLLPPGPMRVDADAARLGQVFVNLIIN
                     AAKFTPPGGRIWVKGGTEGDEAVVHVEDNGAGITHDMLPRIFDLFTQVESTLAQSRGG
                     LGVGLALVKDIVTLHGGSVQVSSAGAGKGSKFSVRLPLAAP"
     gene            complement(6299..6691)
                     /locus_tag="VFO53_03445"
     CDS             complement(6299..6691)
                     /locus_tag="VFO53_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8875360.1"
                     /translation="MSARREGSNEAGGRRAILVVDDNRDLVTSTAELLEVAGYHAIPA
                     YSAREAVDLLDEVERIDLVLSDIRMPGVDGFDLLRVLRHRFPSLPIILMSGLPVTSDD
                     IVPAGAVIVTKPFDPAHLQQCVAAKLAR"
BASE COUNT         1264 a         2277 c         2372 g         1073 t
ORIGIN      
        1 tgacgccgga cgaggcgcgc gggatttact cgaccgagca ggggacgcgg ccggcgcgta
       61 ccgggtcgga cgtcacgccg ggctacatgg gtccggggag ctcgaagggt aagtgagtgc
      121 cgaggcgcgc atcacgcctc gactctctcg cgtgatcgaa acggcggact tcgggccgcc
      181 gtttttgtac gcgcgtgcga agcgaggcgc gcacgtttcg gtgtcgccag ctaggcaccc
      241 cccccccccg gcgctttaga atgccgatcg tgcgcgagga cggcgttccg ccagcaggcg
      301 tgcgcttgcg ctaaatcgag tggcggttgc cgtgccgaaa aaacgcaaga ataacggtcg
      361 ccgcgaaaag tcagcgcgta ccgccgatac acgggacccg acctcgcgcg cgggcaccga
      421 gcctgtggcg ccgtttccga tcgtcggcat cggcgcctcg gcaggcggac tggaggcgtt
      481 caccgagctc ctcgcgcatc tgccgctcga taccggcatg gggttcgtgc tggtccagca
      541 cctcgacccc gatcacgaga gcgcgctccc gcagctgctc ggtcgcgcaa cctcgctgcc
      601 cgtggaggcg gtcaccgaca acacgcgcgt cgcacccaac cgcgtgtacg tcatcccgcc
      661 caacgccagt ctcgtcatag tccagggcgt gctgaagctg aagccgcgcc gtccgaagcg
      721 tgcgccgacg cgcacgatcg acacgttttt cgagtcgctg gcggaagatc agcgcgaatg
      781 cgcgatcggc gtggtccttt cgggcaccgc gagcgacggc acggtggggc tcgaggcgat
      841 caaggccgaa ggcggcatca cgttcgcgca ggacgaaacg gcccgctacg actcgatgcc
      901 gcgcagcgcg gtgaatgcgg gctgcgtcga cttcgtgctt gcgccggaag cgattgcgca
      961 ggagctggcg cgcatcgcca gacacccgta tatcaccggc cgcgcaaacg ctcccgcaac
     1021 gcccgagcag gaccacgcgg aggcgacgtt gcacgaagac gatgccacgc cgttgccatc
     1081 gggcggacac ggcgcgccgc ccacgggtgc gaggcaggcg cgcaacgagg cgacgcgcgg
     1141 cgatgccggc gcaaccgccg gcgaacagcc tgccgcgaat gcgtacaaaa aaatcctgct
     1201 gcaactgcgc agtcattcgg gcgtcgattt ctcgctttac aaatcgagca cgatacagcg
     1261 ccgtgtcgcg cggcgcatcg tgctttccaa gcagtcgagc ccggagagct acgcgcacta
     1321 cctgcgcggc aacacgaagg agctcgacgc gctgtatacg gacgtgctga tcagcgtcac
     1381 gagctttttc cgcaacccgg aagcgttcga cgtgctcaag gaaaaggtgt ttccgacgct
     1441 tctgcagcag cgcggcgacg agccgctgcg cacctgggtg ctcggctgct ccaccggcca
     1501 ggaggcgtac tccattgcga tggcgttcac cgaagcggcc gaaaaggcgc cccgcgttcg
     1561 ccggctgcag atcttcgcca ccgatctcaa cgacgcgctg ctcgagaagg cgcgccgcgg
     1621 gctgtacgcg aagagtctcg ccgacgacat cgagcccgac cgcctgcggc gcttcttcgt
     1681 cgaggaggag gaaggctacc ggatcgtcaa ggcgatgcgc gagatggtgg tgtttgcccg
     1741 gcagaacgtg attgccgatc cgccgttctc gcggatggat ctcgtgagct gccgcaacgt
     1801 gctcatctat ctcgagccgg atttgcagaa gaagctgctg ccggtattcc actacgcgct
     1861 gaagccgcgc ggcttcctgt ttctcggcaa ttcggaatcg atcggcagct tcaccgatct
     1921 cttcgagccg ctgggcagga agagcaagat ttacgtgaaa aaagcggtgg cgacgccgat
     1981 gctccagctc ggcatgcggc gcgaccgcgg cgagcggttg ccgatgcggc ccgtgtccac
     2041 cgcgcccgcc gacgcgacga aggcgttctc cggcgagcag agcgcgcagc gcgaggcgga
     2101 ccgcatcacg gtcaatcagt tcgcgccgcc ggcggtgctg gtcaacgccg atctgcagat
     2161 ccttcagttc cgcggaccga ccggcgcatt tctcgcgcca ccgacgggca aggcgagctt
     2221 cgacgtgctg aagatggcgc gcgagggcct gatgctgccg ctgcgcacgg ccctcgccaa
     2281 ggcgaagaag gacaaccgga ccgtacgcaa ggagaacgtc cgcgtcgatc acgacggcgc
     2341 gagccggacg atcagcctcg aggtcatccc gctgcggaac ctgcacgagc gctgctttct
     2401 gatcgtgttc gaggataccg acaggacgag agtcgccgaa gcggacgcag cggcgcacgc
     2461 cggcaccggg cgcgggaagc gtcgaagccc cgccagcgat gacgcccgcc gcattgccga
     2521 gctcgaatcc acgctcggcg atacgcgcga ctatctgcag tcgctgcagg agcagcacga
     2581 agcggcgaac gaggagctgc aggcgtccaa cgaggaagtg cagtcggcga acgaggagct
     2641 gcagagcatc aacgaggagc tggaaacgtc gaaggaggag ctcgagtcgg ccaacgagga
     2701 gttgtcgacg atcaacgacg agatggccaa tcgcaacacg gagctcaatc gcgcgaacaa
     2761 cgatctcgtc aacctgcaga actcgacgcg actgtcaatc gtgctgctcg ggcgcgacct
     2821 caccattcgc cattacagcc cgcaggccgc gaagcagctc aagctcgttg ccgccgacgt
     2881 cggccggcgc atcggcgccg ttcgccacga tctcgtactt accgacggcg acgccgtgtc
     2941 cgggcaccgg ccggcgggga atggcgtgcc cgggacgggg cgaaacgcgc gcaaggatgt
     3001 cgacctgcgc gggacgccgg cggcgctcga cctggaaggg ttcgtcgcgg aggtcatcga
     3061 ttcggtgcgt gagcgcgagt gccacgtcca ggatcgacat gggcgctggt acctgctgcg
     3121 cgcccgcccg tacctgacac tcgacaagca cgtcgacggc gccgtgctcg tgctgaccga
     3181 catcgacgag ctgacgcgcc atacgcaaga cattgccgcg gcgcgcgact tcgcggaagc
     3241 gatcattcgt accgtgcgcg atccgttcgt catcctggac gccggcctgc gcgtggagcg
     3301 ggccaacgac gcgttctacg acgcgttcgg ggtcgcgccg gcggagacgg aagggcgcgt
     3361 gatcttcgat ctcggcaacg gccagtggaa cgttccccgc ctgcatgaat tgctcgagga
     3421 aatcctgccg gggaagagcg tgttcaacgg tttcgaggtg acccacgact tcgcaggcat
     3481 cggccggcgg aagatgctgc tcaatgctcg cgctcttcaa cgcgccgacg gccgtccggc
     3541 gcggatcctc gtcggcatgg aggatgtgac cgagaaggaa gccgtggagg aggcgcggcg
     3601 tgcaggcgaa acgcattacc gaatgctgtt cgcggcggcg ccgatggcca ttttcgtctg
     3661 cgaccgcgac ggcgtgatcc ggtattacaa ccggcgcgcg gccgagctgt ggggacggga
     3721 gcccgagatc ggcgtcgagc gctattccgg cgcggtgaag ctatggctgc ccaatggcgt
     3781 gccgttgccg cacgaacaca gtccgatggc cggcgtgcta gccaccgggg tcagcgcgca
     3841 taacgtcgag ttggagatcg agcgccccga cggatcgcgc ctttcggtgc tcgagaattt
     3901 ctcgccgttg aagaacgaaa gcggcgcaat caccggcgcg atcacgtcgt tcatggacat
     3961 caccgagcag aaggacgcga ccaacgcgtt gcagcggctg tccgtcgagc tcctcgacgc
     4021 cgaccggcgc aagaacgaat tcctggcgat gctggcgcac gagctgcgca atccgctcgc
     4081 tccgatccgc acggcgctga cactgatgcg ccggacgaca aagagcgccg gcggctcgcc
     4141 gcaaccggcc gtcgacatga tcgatcgcca ggtcggccag ctggtacggc tggtggacga
     4201 cctcctcgac gtgagccgca tcagccgcgg gcagatcgag ctgcaacgcg acgagatcga
     4261 gctcgcatcg accatgcatc gcgccgtgga gactgcccgg ccgctgctcg acgcgatgga
     4321 gcacgagctg acgctcgtgc tgcccgccga gccgatcacc gtcgacgccg atgcgatccg
     4381 gctcgcgcag atcgtcggca acctgctcaa caacgcgtcg aagtttaccg atcgcggcgg
     4441 acgcctccgg ctcgccatcg aacgcgacgg cggcgacatc gtgattcgtg ttgccgacaa
     4501 cggcatcggc atcgacaaac gccagcttcc gcgcgtgttc gacctcttca tgcaggtcga
     4561 cacgtcgctc acgcgctcgg ccaccggcct cggcatcggg ctgggtctcg tgaagcagct
     4621 cgtgacgctg cacgggggaa gcgtcgaagc gcgaagcggc ggcgtcggtc agggaagcga
     4681 gttcatcgtg cgcctgccca ttgtcgtgac ggcgccgatg cgcccggcag cgggagcggg
     4741 cggcgactcg ccggtcaacg ccgccgcagg gcgcatcctg atcgccgacg acaatcgcga
     4801 tgcggcgagc agcatcgcct cgctgcttcg gctcgacggc aacgtcaccg aaaccgcgca
     4861 cgacggactc gcggcattca cggcggccga ggcgttccgc cccggcgtga tcctcctcga
     4921 catcggcatg ccgaagatga atggctacga tgtcgcacgc aggattcgcg ctgcatcctg
     4981 gggccgcgac atcgtgctga tcgcgctgac cggctggggt cagcccgaag accgcgagaa
     5041 gtcacgggaa gcgggattcg atcatcatct ggtcaagccc gtggacccgt ccgcgctgac
     5101 gcggctgctt gccgggattg cggagcgatc gaaatgacgt cgagctcggc gaacgatgcg
     5161 ccggcggagc aacttcttcg cctgctggtc gagcagacga cggagtacgc gatcatcttg
     5221 ctcgatgtgg atggagatgt catcgggtgg aacaagggcg ccgcgcatct gttcggcatt
     5281 gcgcgcgacg acgcgatcgg tcaacccgcg tcgctgatct ttaccgcgca ggacatcgca
     5341 cgcggcacgc cgcagcagga aatcgcggcc gccgaagccg acaccgccgc ggaggacgac
     5401 cgctggctcg cgcgttccga tggctcccgc ttctgggcgg cgggcgtcat gattccgctc
     5461 catgacgagc agggacggct tgccggcttc ggcaagatcc tgcgcaaccg gaccgacttg
     5521 cgggaacaga tcgatacgct gcgcaatcag gccgatgcgt ccgacgcgat ggtcgtgcgc
     5581 aaggacttgt tcctgacgac gttgtcgcac gagcttcgca acccgctcgg gccgctggtc
     5641 catgcaacgg aaatcatacg caggaagctg gccgccggcg cgggaatcga agacacgctc
     5701 gggatcatcg atcgccaggt cgcgagcctc gtgcggctgg tcgacgatct gctggatctg
     5761 tcgcgggtca gcgcggggcg gatggcgctt cagaaggaac gcgtcacgct ccaggacatc
     5821 gtcgatcgcg cggtggaaag cgcaggcacg ctcgtgcgcg agcaacgcca tcagttggac
     5881 gtgttgctgc cgccgggccc gatgcgcgtc gatgccgatg ctgcgcgcct cgggcaggtg
     5941 ttcgtcaacc tcatcatcaa cgccgccaag ttcacgcccc ccgggggtcg catctgggtc
     6001 aagggcggca ccgaaggcga tgaggcggtg gtgcatgtcg aggacaacgg cgccggcatc
     6061 acgcacgaca tgctgccgcg cattttcgac ctgttcacgc aagtcgaatc gacgctcgcg
     6121 caatcacggg gagggctggg cgtcggcctt gcgctcgtca aggatatcgt tacccttcac
     6181 ggcggcagcg tgcaggtcag cagcgcgggc gcaggcaagg gcagcaagtt ttcggtgcgc
     6241 cttccactcg cagcgccgta agcgggcggc cgcgcggtcc catctcgcta gcgtcaatct
     6301 agcgggcgag ctttgccgcg acgcattgct gaaggtgcgc aggatcgaag ggcttggtca
     6361 cgatcacggc gcccgccgga acgatgtcgt cggacgtcac cggaagcccg ctcatcagga
     6421 tgatcggcag cgacgggaat cgatgccgca gaacgcgcag tagatcgaag ccgtcgacgc
     6481 ccggcatgcg gatgtccgac aggacgagat cgatcctttc gacttcatcg agcagatcaa
     6541 cggcctcgcg tgccgagtac gccgggatgg catggtagcc ggccacctcg agcagttcgg
     6601 cagtggacgt tacgagatcg cggttgtcat cgacgacgag aatcgcacgg cggcctcccg
     6661 cctcgttcga accttcgcga cgcgcagaca atgcggccaa attcgtccaa tccgtcggcg
     6721 ccggatcatt tccggagatc gcattgtaac gtcggaaaaa catcccgccg cacttcggtg
     6781 aacccggaat gcggcgggga ctcaatcgcg accggcgcgg gtcgcttcag ttgcagggat
     6841 tggccagcgg ctcaccacag tcgggcgtct gctggtggag gttaccgagc gtgtattcat
     6901 gcgcgagcgc aggcgtcgcg ccgatgagga gtgcggcgag cgccgcaacg aactgcatcc
     6961 tggtgggccg gccattcgtc cttcgg
//
LOCUS       DASSVX010000073         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_685769, whole genome shotgun sequence.
ACCESSION   DASSVX010000073 DASSVX010000000
VERSION     DASSVX010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685769"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /gene="uvrA"
                     /locus_tag="VFO53_03450"
     CDS             <1..1698
                     /gene="uvrA"
                     /locus_tag="VFO53_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET8875361.1"
                     /translation="GTRLRREARNVKVGDGADARPIYEISAWPLKESSAYFSRIKLPG
                     HKAAIADRIVKEIVSRLNFLNNVGLDYLSLDRSAETLSGGEAQRIRLASQIGSGLTGV
                     MYVLDEPSIGLHQRDNDRLLTTLKHLRDLGNSVIVVEHDQDAILTADYVVDMGPGAGE
                     HGGQVVAQGTPDDIRRAPHSLTGQYLAGHRRIPTPERRHVADPERMLTITGASGNNLR
                     DVTLALPVGLFVCVTGVSGSGKSTLVNDTLYHAVARHLYDSAEEPAPYADIDGLEHYD
                     KVINVDQSPIGRTPRSNPATYTGLFTPIRELFAQVKEARERGYGPGRFSFNVKGGRCE
                     ACQGDGLIKVEMHFLPDVYVPCDVCHGQRYNRETLEIRYKDRNIHDVLSMTVEQAYAF
                     FEAVPSVARKLSTLLDVGLGYITLGQSATTLSGGEAQRVKLALELSKRDTGRTLYILD
                     EPTTGLHFHDIEMLLTVLHRLRDHGNTVVVIEHNLDVIKTADWIIDLGPEGGAGGGQV
                     IAQGTPEAIAGVDASYTGRYLRPVLVREGAATNGTTGKRRPGAASVRGRKVSGDLLG"
     gene            complement(1706..2089)
                     /locus_tag="VFO53_03455"
     CDS             complement(1706..2089)
                     /locus_tag="VFO53_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET8875362.1"
                     /translation="MVVAAFASWHENHERARRALDGEVRLIEHCALETYSVLTRLPAP
                     HRAPDAVVGEFLLTRFSRPYLRLSANDYRNFILALSDHGIAGGAAYDALIAATAVACG
                     ARLVTCDRRALSIYERYEVATQLVC"
     gene            2373..>2637
                     /locus_tag="VFO53_03460"
     CDS             2373..>2637
                     /locus_tag="VFO53_03460"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HET8875363.1"
                     /translation="MNRIIDDADRMVDDCLRGVLAAHPDLLAATSDPRVVRAASAPRP
                     GRVGVVTGGGSGHEPAFVGYVGPGLCDAVAIGEIFTSPSARAFQ"
BASE COUNT          457 a          871 c          893 g          416 t
ORIGIN      
        1 ggcacgcgcc ttcgtcgcga ggcgcgcaac gtgaaggtcg gcgacggcgc cgacgcgcgg
       61 ccgatctacg agatctccgc ctggccgctc aaggaatcga gcgcgtattt ctcgcgcatc
      121 aagctgcccg ggcacaaggc cgcgattgcc gatcggatcg tcaaggagat cgtgtcgcgg
      181 ctcaacttcc tgaacaacgt cggcctcgat tacctgtcgc tcgatcgctc ggcggagaca
      241 ttgtcgggcg gcgaggcgca gcgcatccgg ctcgcctcgc agatcggctc ggggctgacc
      301 ggtgtcatgt acgtgctcga cgagccgtcg atcggcctgc atcagcgcga caacgaccgg
      361 ctgctgacaa cgctcaagca tctgcgcgat ctcggcaaca gcgtgatcgt cgtcgagcac
      421 gaccaggatg cgatcctcac cgccgactac gtcgtcgaca tgggtcccgg tgcgggcgag
      481 cacggcgggc aggtcgttgc gcaaggcacg cccgacgaca tccggcgcgc gccgcactcg
      541 ctgacgggtc agtacctggc cgggcatcgg cgcattccaa cgcccgagcg gaggcacgtg
      601 gccgaccccg agcggatgct gacgatcacc ggcgccagcg gcaacaacct gcgcgacgtg
      661 acgctcgcgt tgccggtggg gctcttcgtg tgcgtgaccg gcgtgtcggg ctcgggcaag
      721 tcgacgctcg tcaacgacac gctctatcac gcggtcgcgc ggcatttgta cgacagcgcg
      781 gaggagcccg cgccgtacgc cgatatcgac gggctcgagc actacgacaa ggtgatcaac
      841 gtcgaccaga gtccgatcgg ccgcacgccg cgctcgaatc cggcgaccta cacgggcctg
      901 ttcacgccga tccgggaact gttcgcgcag gtgaaggaag cgcgcgagcg cggctacggc
      961 ccggggcgct tctcgttcaa cgtcaagggt ggacggtgcg aagcgtgcca gggcgacggc
     1021 ctcatcaagg tcgagatgca cttcctgccc gatgtttacg tgccgtgcga cgtgtgccac
     1081 ggccagcgct ataaccgcga gacgctcgag atccgctaca aggaccgcaa catccacgac
     1141 gtgctgtcga tgaccgtcga gcaggcatac gcattcttcg aggcggtgcc gagcgttgcg
     1201 cgcaagctgt cgacgctgct cgacgtggga cttggctaca tcacgctcgg ccagtcggcg
     1261 acgacgcttt ccggcggcga ggcgcagcgc gtgaagctgg cgctcgagct gtcgaagcgc
     1321 gacaccggac gcacgctgta catcctcgac gagccgacca ccggattgca cttccacgac
     1381 atcgagatgc tgctgaccgt gctgcatcgg ctgcgcgacc atggcaacac ggtcgtcgtg
     1441 atcgagcaca acctcgacgt gatcaagacg gccgactgga tcatcgacct cggtcccgag
     1501 ggcggcgccg gcggcggcca ggtgattgca caggggacgc cggaagcgat cgccggcgtc
     1561 gatgcgagct acacgggacg ctacctgagg ccggtgctcg tgcgcgaggg agcggcaacg
     1621 aacggcacga ccggcaagcg tcgtcccggc gcggcgtcgg tgcgagggcg caaggtgtcg
     1681 ggcgatctgc tcggctgacg atgcgtcagc aaaccagctg ggttgctact tcataacgct
     1741 cgtagatcga aagcgcgcgc cgatcgcagg taaccaaccg cgcgccgcac gccacggccg
     1801 ttgctgcgat cagcgcgtcg taagccgcac caccggcgat gccgtggtcg gacagtgcaa
     1861 gaatgaagtt gcgatagtcg ttcgcgctca accgcagata gggtcgcgag aatcgcgtca
     1921 gcaggaactc gcccactacg gcgtccgggg cgcgatgggg agcgggcagg cgggtaagca
     1981 ccgaataggt ctcgagcgcg caatgttcga tcaggcgcac ttcgccgtcg agagcacgtc
     2041 gcgcacgttc gtgattttcg tgccaggacg cgaaggcggc gaccaccacg ctagtgtccg
     2101 ccgtcttcaa cgccgggttc gctcgagagt cgcacgaacc aggtcggacg taagcacagg
     2161 caaatcggcg tccggaacgg cgaccactgc gatgccacgc cctttcaatt gcataggcgt
     2221 tcgcgcgatc tcgatctcca gtcgcccatc tcccgcgtga atttcgagcg cttggccggg
     2281 cgcaatgact ggcgcccgtg tatatttcac gcaccaatcg tctgtcgttt tctgttcggg
     2341 cacgggcgat gcgcataccg cggaggcagc cggtgaacag gatcatcgat gacgccgacc
     2401 ggatggtcga cgactgcctg cgcggtgtgc tcgccgcgca tcccgatctg ctcgccgcga
     2461 ccagcgaccc acgcgtcgtt cgcgccgcgt cggcgccgcg accgggacgc gtcggcgtcg
     2521 tgacgggtgg cggttccggc cacgagccgg ccttcgtggg ttacgtcggg cccggcctgt
     2581 gcgacgcggt ggcgatcggc gagatcttca cgtcgccctc cgcacgcgcg ttccagc
//
LOCUS       DASSVX010000074         8149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_319211, whole genome shotgun sequence.
ACCESSION   DASSVX010000074 DASSVX010000000
VERSION     DASSVX010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319211"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFO53_03465"
     CDS             <1..274
                     /locus_tag="VFO53_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HET8875364.1"
                     /translation="VGFWPGSGDVPSAAFYAYASPEPGGYGECAMRPGQAFYDHALGE
                     FILMYDDVRRASSPCAALLEFCNSTYGAAAELGRWDRAALERQLRA"
     gene            309..599
                     /locus_tag="VFO53_03470"
     CDS             309..599
                     /locus_tag="VFO53_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HET8875365.1"
                     /translation="MDTHCTHLDEIKLTHTRTRVCDECVRLGDTWVHLRLCLECGNVG
                     CCDSSKNRHATAHFHRTRHPLVRSLEPGERWIWCYVDEIVPGELPDEGIDAR"
     gene            596..1315
                     /locus_tag="VFO53_03475"
     CDS             596..1315
                     /locus_tag="VFO53_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HET8875366.1"
                     /translation="MNPDSQLLAALGGITTATLTTVLLKKGLRNVYLRHTKPVRERQA
                     RVAGRAFTLRFIPAREDQATPESWAKPTSTRGAIEAMPAGCVAVVDARGVTDAGIFGD
                     ILCARMQARGVAALVTDGVLRDVRGVLDTQLPIWCAGAAAPPSVAGLTFVNWQEPIGC
                     GGAAVFPDDVVVADDDGAVVIPAALVDEVIALATEQERLEAWIMAEVARGVPLPGLYP
                     PSAETRARYDAQRGDSKGRRE"
     gene            1312..2286
                     /locus_tag="VFO53_03480"
     CDS             1312..2286
                     /locus_tag="VFO53_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017741923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8875367.1"
                     /translation="MNRNRLGRTQFEVAPLAFGGNVFGWTADEATSFRLLDAFVEAGG
                     NLIDTANSYSRWVPGHEGGESETVIGRWVARRGRHDDVVIATKVGSDMGQGGKCLRRD
                     YILQAVDESLRRLQVDAIDLYQSHWDDESTPFDETLDAYARLLEQGKVKAIGASNLTP
                     RRLQQALDASARDGLPRYQTLQPLYNLYDRDTFEGPLCELCLREDIGVITYFSLAAGF
                     LTGKYRSEQDYAKSARGPGMKKYLNRRGRRILDALDDVAGRLHAKPVQVALAWLMAQP
                     GVTAAIASATSLPQLAELVGATRLSLDELDMRQLDEASRATSDAGQGP"
     gene            complement(2299..3516)
                     /locus_tag="VFO53_03485"
     CDS             complement(2299..3516)
                     /locus_tag="VFO53_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8875368.1"
                     /translation="MPHGEVESAYAWTRLGVAVLLSTIGGVGMWSVVVALPAVQAEFA
                     VARADASLPYTLTMICFGLASIGMGRLSDRFGIMLPVALGTLLLAAGYAVAAVAPDLR
                     DYTLAQGLLIGAGSAATFAPLLAHVSLWFTRRRGIAVAIFASGNYLAGTVWPPIVEHF
                     IGTSGWRPTYVGIGIFCLVAMMPLTLLLRRKAPVADGPETAKRMRRASPQTLGISGNA
                     LQALLVIAGLSCCVAMSMPQVHLVAYCSDLGYGPARGAQMLSLMLACGIVSRLVFGVI
                     CDRIGGLRTLLLGASLQGAALVLFLPFNGLVSLYVISALFGLFQGGIVPAYAIIIREF
                     FSRAEAGARVATVITATLFGMALGGWMSGAIFDVTGSYRAAFVNGLLWNLVTVSIAAW
                     LLRRSIGRLALAG"
     gene            complement(3749..4174)
                     /locus_tag="VFO53_03490"
     CDS             complement(3749..4174)
                     /locus_tag="VFO53_03490"
                     /inference="COORDINATES: protein motif:HMM:NF019696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HET8875369.1"
                     /translation="MKYMIGCICAACALGLAGCNTMEGLGTDISKGGQKIQSTAENVR
                     HDWHQASVRNEREYDQARATCAGLSGTDHDVCMDRARERYTTSMNDARREYPRTSMAT
                     ESQEDRMEDAFDAARSGCESLRGTAFDQCIADARARYHQ"
     gene            complement(4314..4763)
                     /locus_tag="VFO53_03495"
     CDS             complement(4314..4763)
                     /locus_tag="VFO53_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET8875370.1"
                     /translation="MRTPAAVAGHPIHPMLVTLPIGLWVFSFVCDLFFVFGSGAPVWS
                     TVALYTMAGGIVGALAAAIPGFIDLLSLPAGPRKTALVHMAINLTIVVLYVINFWRRT
                     GTPDNPGGYVWLSLLTILMLLVSGWLGGRMVYVLGVAVAPASEPRQP"
     gene            4923..6113
                     /locus_tag="VFO53_03500"
     CDS             4923..6113
                     /locus_tag="VFO53_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8875371.1"
                     /translation="MSRSTKTWNRREFIGGVAAAAGAAAFPRPVFAADPVRIGLVIPK
                     TGPFASTGHQVEAGCRLYLAKNGDTVAGRTIELIVRDDTGTAPELTKRIAQELVVRDN
                     AHVLAGFGLTPLAFAAAPVATETRVPMIVMAAATSSIPQKSPYIVRSGFTLPQVTAPL
                     AEWAAKNGIRTAVSLVSDYGPGIDAEKTFVRRFTEGGGTIAEAVRAPLANPDFAPFVQ
                     RAKDAKADALFVFVPSGAGTAVMKQYAERGLRDAGTKLICTGDVVDDDILDAMGAPAL
                     GVVSSHHYSAAHPSAQNKAYVAAFMQANAGMRPNFMSVGGYDGMHVIHEALTKTRGDV
                     DGDKLVAAMKGMTWESPRGPMSIDAATRDVIQDVYIRKVERVDGKLFNVEFATIPHVK
                     DPGA"
     gene            6205..6462
                     /locus_tag="VFO53_03505"
     CDS             6205..6462
                     /locus_tag="VFO53_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HET8875372.1"
                     /translation="MDKSPAERQAEHQVLEHIQDLVAEEHRLLEKRAAGDADHDRLTR
                     VQIELDQCWDLLRQRRALRETGADPGHAEIRAPDVVEKYIG"
     gene            complement(6471..7751)
                     /locus_tag="VFO53_03510"
     CDS             complement(6471..7751)
                     /locus_tag="VFO53_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET8875373.1"
                     /translation="MKTRRSRVGVAVALAVATGGLLGVAPALFAREGSHAQIERGRYL
                     AQAGDCAACHTAERGQPFAGGRAVPTPFGVIYSTNITPDDSTGIGNWTSDDFYRAMHA
                     GIAPGGKHLYPAFPYPWYTKLTRADVDAIRSYLATLSPVRQENKPTELPWPFSVREAM
                     AGWNALYFKEGTFRSNPQKSAEWNLGAYLVEGLGHCGACHSPKNFAGAPKRDKAFQGG
                     YGENCYATSLGGDLHDGLGQWSTDDIVQYLKTGVNKRAAAFGPMSEVVHDSTQHLDEN
                     DLHAMAVYLKDLPPHESPANPASTSAAIGNTSDGDRVARGHGLYIDDCAGCHMDNGEG
                     IAGVFPPLKGNNVVQAKDPETVVHVILTGAKTAVTQQKPTGLAMPAFDWKLSNDQVAD
                     LVTYLRGAWGNHGDAVDAGKVADTRSTLVAARTK"
     gene            complement(7748..>8149)
                     /locus_tag="VFO53_03515"
     CDS             complement(7748..>8149)
                     /locus_tag="VFO53_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HET8875374.1"
                     /translation="RDEYGNPLLRLTFDFHPNEHLMSAFLTEKAAGIAKAMKPRHMDA
                     NPRQGHYTIVPYQTTHNTGGAIMGTDPRTSVVNRYLQSWDVPNLFVVGSCAFPQNAGY
                     NPTGTVGALAYWAANAIRTQYMKKPGALVSA"
BASE COUNT         1398 a         2689 c         2806 g         1256 t
ORIGIN      
        1 tgtggggttc tggccgggaa gcggcgacgt gcccagcgcc gctttctatg cctacgcgtc
       61 gcccgagccc ggcggttacg gcgaatgcgc aatgcggcct ggccaggcgt tctacgacca
      121 cgcactcggc gaattcatcc tgatgtacga cgacgtgcga cgcgcgtcgt cgccgtgcgc
      181 ggcgctgctc gagttctgca attccacgta cggcgcggcc gccgagctcg gccgctggga
      241 ccgcgcagcg ctcgagcggc aactccgagc gtgagtgcca gttactgtaa cgccacgaag
      301 gatcaccgat ggacacgcac tgcacgcatc tcgatgaaat caagctgacg catacgcgca
      361 cacgggtctg cgacgaatgc gtgaggctcg gcgacacgtg ggtgcatctg cgcctgtgcc
      421 tcgaatgcgg gaacgtcggc tgttgcgatt cgtcgaagaa ccggcacgcg accgcgcatt
      481 ttcatcgcac gcggcatccg ctcgtgcgct cgctcgagcc gggcgagcgc tggatctggt
      541 gctacgtcga cgagatcgtg ccgggcgagc tgcccgacga agggatcgac gcgcgatgaa
      601 cccggatagc caactgctcg ctgcgcttgg cggcatcacg accgcaacgc tgacgaccgt
      661 cctgttgaag aagggcctgc gcaacgtcta tcttcgccat acgaaaccgg tccgcgagag
      721 gcaagcccgc gtcgcgggac gcgcattcac gcttcgcttc attccggcgc gcgaggatca
      781 ggcaacgccc gaatcctggg cgaagcccac gtccacgcgt ggcgcgatcg aggcgatgcc
      841 ggccggatgt gtcgccgtcg tcgacgcgcg cggggtcacc gatgccggaa tcttcggcga
      901 catcctgtgc gcgcggatgc aggcacgcgg cgtcgcggcg ttggtcaccg acggcgtgct
      961 gcgcgatgtg cggggcgtgc tcgacacgca gctgccgatc tggtgcgccg gtgctgcggc
     1021 gccgccgtcc gttgcgggcc tgacgttcgt caactggcag gaaccgatcg gctgcggtgg
     1081 cgcagccgtt tttcccgacg atgtcgtcgt cgccgatgac gacggtgcgg tcgttatccc
     1141 cgcggcactc gtcgacgaag tgatcgcgct tgcaacggag caggagcggc tcgaggcgtg
     1201 gatcatggcc gaggttgccc gcggcgtgcc gctgcccggg ctctatccgc cgagcgccga
     1261 aacgcgggcg cgctacgacg cgcagcgcgg cgattcgaaa ggcagacgcg aatgaatcga
     1321 aacaggcttg gccgcacgca attcgaggtc gccccgcttg cgttcggcgg caacgtgttc
     1381 ggctggacgg ccgacgaggc gacatcgttc cggctgctcg acgccttcgt cgaggccggc
     1441 ggcaacctga tcgataccgc gaattcgtat tcgcgctggg tgccgggtca cgagggcggc
     1501 gaatcggaaa ccgtgatcgg acgctgggtc gcgcggcgcg gacgccacga cgacgtggtc
     1561 atcgcgacga aggtcggttc ggacatgggg cagggcggga agtgcctgcg ccgcgactac
     1621 atcctgcagg cggtcgacga atcgctgcgc cgcctgcagg tcgacgcaat cgatctctat
     1681 caatcgcact gggacgacga gagcacgccg ttcgacgaga cgctcgacgc gtatgcgcgg
     1741 ctgctcgagc agggcaaggt gaaggcgatc ggcgcgtcga acctcacgcc gcggcggctt
     1801 cagcaggcgc tcgacgcgag cgcgcgcgac ggcttgccgc gctaccagac gctgcagccg
     1861 ctctacaacc tctacgatcg cgatactttc gaaggcccgc tttgcgagct gtgcctgcgc
     1921 gaggacatcg gcgtcatcac gtacttctcg ctcgccgcgg gctttctcac ggggaaatac
     1981 cggagcgagc aggattacgc gaagagcgcg cgcgggccgg gaatgaaaaa gtacctgaac
     2041 cggcgcggcc ggcgcatcct cgacgcgctc gacgatgttg ccgggcggct gcatgcgaaa
     2101 ccggtgcagg tcgcactcgc gtggctgatg gcgcagccgg gcgtgaccgc cgcgatcgca
     2161 agcgcgacgt cgctgccgca gcttgccgag ctcgtcggcg cgacgcggct gtcgctggac
     2221 gagctcgata tgcggcaact cgacgaggcg agccgagcaa cgagcgacgc aggccagggc
     2281 ccgtgaccgg gtcctgattc agcccgcgag cgccaggcgg cctatcgaac ggcgcagcag
     2341 ccacgccgcg atcgagacag tgacgagatt ccacaggagg ccgttgacga acgccgcgcg
     2401 ataagagccg gtgacgtcga agatggcgcc cgacatccat ccgccgagcg ccatgccgaa
     2461 cagcgtggcg gtgatcaccg tcgcaacgcg cgcgccggct tccgcgcggc tgaagaattc
     2521 gcggatgatg atcgcgtacg ccggcacgat gccaccctgg aagagaccga acagcgccga
     2581 gatcacatag agcgagacga gcccgttgaa cggcaggaac aggacgagtg ccgcaccctg
     2641 cagcgacgca cccagcagca gcgtgcgcaa gccgccgatc cgatcgcaga tcacgccgaa
     2701 cacgagccgg ctcacgatgc cgcatgcgag catcagcgac agcatctgcg ccccgcgcgc
     2761 gggcccgtag cccaggtcgc tgcagtaggc gacgaggtgt acctgcggca tcgacattgc
     2821 gacacagcac gagaggccgg cgatgacgag caacgcctgc agcgcgttgc cactgatgcc
     2881 gagcgtctgc ggcgacgcgc ggcgcatgcg cttcgccgtc tcgggaccat ccgcgaccgg
     2941 cgccttgcgc cgcagcagca gcgtgagcgg catcatcgcc acgaggcaga agatgccgat
     3001 tcccacgtac gtgggacgcc atccgctcgt gccgatgaaa tgctcgacga tcgggggcca
     3061 gacggtgccc gcgagatagt tgccgctcgc aaagatggcg accgcgatgc cgcgccggcg
     3121 cgtgaaccac agcgacacat gcgcgagcag cggtgcgaac gtcgcggcgc tgccggcgcc
     3181 gatcaggagg ccctgcgcaa gcgtgtagtc gcgcagatcg ggcgcaaccg cggcgaccgc
     3241 gtaaccggcc gcgagcagca gcgtcccgag ggccacggga agcatgatgc cgaagcgatc
     3301 ggacagccgg cccatgccga tgctcgcaag gccgaagcag atcatcgtca gcgtataggg
     3361 aagcgacgca tcggcacgcg cgacggcgaa ttcggcctgc accgcaggca gcgcaacgac
     3421 gaccgaccac atgccgacgc cgccgatcgt cgacagcagc accgcgacgc cgaggcgcgt
     3481 ccatgcatag gcggattcga cttcgccgtg gggcatcgat cttcctgagg tgacggggca
     3541 aggcgcggtc cgtcatgcac ggcgcgcatc ggcgcgcgga cgacatgcat gcgaaatcgc
     3601 gccgcgccat gttacacgcg gcgcgcgata ccccggtgtc ccggtgcgcg aagcggacgc
     3661 gctcgtgcgg cgcgaagcgg atcaacgcgc gaagcgggca atcggacgac tgcccgcttc
     3721 gcggaagacg gcccgccggg aaacagaact actggtgata ccgtgcgcgc gcgtcggcga
     3781 tgcactgatc gaaggccgtc ccgcgcagcg actcgcagcc gctgcgcgcg gcgtcgaacg
     3841 catcctccat ccggtcttcc tgcgactcgg tggccatgct ggttcgcgga tattccctgc
     3901 gtgcgtcatt catcgacgtg gtgtagcgct cgcgtgcgcg atccatgcat acatcgtggt
     3961 cggtgccact caaacccgcg cacgtggcgc gcgcctggtc gtattcgcgc tcgttgcgaa
     4021 cgctggcctg atgccagtcg tggcgcacgt tctcggccgt gctttgaatc ttctggccac
     4081 ccttcgagat gtcggtgccg aggccttcca tggtgttgca gccggcgagg ccgagggcgc
     4141 aggccgcgca aatgcatcca atcatgtact tcatgttcgc tccgttgagg ttggaatcga
     4201 cgccgtcgat gcggcccgga cgctcgcgtt acgcgggcac gggcaaatcg gccgcttcgc
     4261 gaagttggag gccgcgcggg aggtgaagtt cggatcgccg gcgccgcgca gcactacggt
     4321 tgccgcggtt ccgatgcggg tgccaccgcg accccgagca cgtacaccat ccggcctccg
     4381 agccagccgg aaaccagcag catcaggatc gtcagcaacg aaagccacac gtagccgccg
     4441 ggattgtcgg gtgttccggt tcgccgccag aagttgatga catagagaac aacgatcgta
     4501 agattgatcg ccatgtggac gagcgccgtt ttgcgcgggc cggccggcag cgaaagcaga
     4561 tcgatgaatc cgggaatcgc cgcggcgagc gccccgacga tgccgcctgc catcgtgtag
     4621 agcgcgacgg tgctccacac gggcgcgccg ctgccgaaga cgaagaaaag gtcacagacg
     4681 aacgaaaaga cccacaggcc gattggaagc gtgaccagca tcgggtggat cgggtgcccg
     4741 gcaactgcgg ctggcgtgcg catggaacct ccaagagagg ggatcgagga ccggtgtggg
     4801 ttggacgcgc gtgcaaggat ggcgttccct ggcgcgcctc gcgtgcgcgc cggcgggccc
     4861 tgcgggcgcc attccgctac catccgcttc tctgccgatt accgtccgac ggagcatcca
     4921 ccatgagccg cagcacgaag acctggaacc ggcgcgaatt catcggcggt gttgccgcgg
     4981 cagcgggcgc ggcagcgttc ccgcgacccg tcttcgcggc cgatccggtg cgcatcggac
     5041 tcgtcatccc gaaaacgggt ccgtttgcct cgaccggcca ccaggtggag gcgggctgcc
     5101 ggctctacct cgcgaagaac ggcgacacgg tcgccggccg cacgatcgag ctgatcgtgc
     5161 gtgacgacac cggcaccgcc cccgagctca cgaagcggat cgcgcaggag ctcgtcgtgc
     5221 gcgacaacgc gcacgtcctt gcgggcttcg ggctcacgcc gctcgccttc gccgcggcgc
     5281 cggtcgccac cgagacacgc gtgccgatga tcgtgatggc cgccgccacg tcatcgatcc
     5341 cgcagaaatc gccgtacatt gttcgctccg gattcacgct gccgcaggtg accgcgccgc
     5401 tcgccgaatg ggccgcgaag aacggcattc gcaccgccgt ctcgcttgtc agcgactacg
     5461 gaccggggat cgacgcggag aagacgttcg tgcgccgctt caccgagggc ggcggaacga
     5521 tcgcggaggc ggtgcgtgcg ccgctcgcca atcccgattt cgcgccgttc gtccagcgcg
     5581 cgaaagatgc gaaggccgac gcgctcttcg tgttcgtgcc gtccggcgcg ggcaccgccg
     5641 tgatgaagca gtacgccgag cggggattgc gcgacgcagg cacgaagctc atctgcaccg
     5701 gcgacgtggt cgacgacgac attctcgatg caatgggcgc gccggcgcta ggcgtggtga
     5761 gctcgcatca ctattctgcg gcgcatccgt cggcgcaaaa caaggcctat gtcgcggcat
     5821 tcatgcaggc caatgccggg atgcggccga acttcatgtc ggtcggcggc tacgacggca
     5881 tgcacgtcat ccacgaagcg ctgacgaaga cgcgcggcga cgtcgacggc gacaagctcg
     5941 tcgccgcgat gaaggggatg acgtgggaaa gcccgcgcgg accgatgtcg atcgacgcgg
     6001 caacgcgcga cgtgatccag gacgtctaca tccgcaaggt cgagcgagtc gacggcaagc
     6061 tcttcaacgt cgagttcgca acgatcccgc acgtgaagga cccaggcgcg tgacgcgggg
     6121 ttccgcggcg cgtcgcgccg ggaatctttg gcgcgcggca gaagtcgaat gccgaaccca
     6181 ctcgcgagcg gagaacgaac ggccatggac aagtcaccag cggagcgcca ggcggaacac
     6241 caggtgctcg aacatatcca ggatctcgtc gccgaggagc accggctgct cgagaaacgt
     6301 gcggctggcg atgccgacca cgatcggctg acgcgggtgc agatcgagct cgaccagtgc
     6361 tgggatctgc tgcgtcagcg gcgtgcgctg cgcgagaccg gcgccgatcc cgggcacgcc
     6421 gaaatccgtg cgcccgacgt cgtggagaag tacatcggct gaaaccttcg ttacttggtg
     6481 cgcgcggcca ccagcgtgct gcgcgtgtcg gccaccttcc ccgcatcgac ggcatcgcca
     6541 tggttgcccc aggcgccgcg cagatacgtc acgagatcgg cgacctgatc gttggagagc
     6601 ttccagtcga acgccggcat cgcgaggccc gtcggctttt gctgtgtcac ggccgttttc
     6661 gcgccggtca ggatgacgtg cacgaccgtc tcgggatcct tcgcctgcac gacgttgttg
     6721 cccttgagcg gcgggaacac gcccgcgatg ccctcgccgt tgtccatatg gcagccggcg
     6781 cagtcgtcga tgtagaggcc gtgcccgcgg gcgacgcggt cgccatcgga cgtgttgccg
     6841 atggcggcgc tcgtcgacgc tggattcgcc ggtgattcgt gcggcggcag gtccttgaga
     6901 tagaccgcca ttgcgtgcag gtcgttttcg tcgaggtgct gcgtcgagtc gtgcaccacc
     6961 tcggacatcg ggccgaacgc cgccgcccgc ttgttcacac cggtcttcag gtactgcacg
     7021 atgtcgtcgg tcgaccactg gccgagaccg tcgtgcagat cgccgccgag gctcgtcgcg
     7081 taacagttct cgccgtaccc gccctggaag gccttgtcgc gcttcggcgc cccggcaaag
     7141 ttcttcggcg aatggcatgc gccgcagtga cccagtccct cgacgagata ggcgccgagg
     7201 ttccattcgg cggacttctg cggattggat ctgaacgtgc cctccttgaa atagagcgcg
     7261 ttccacccgg ccatcgcttc gcgaacgctg aacggccacg gcagttccgt cggcttgttc
     7321 tcctggcgta ccggggagag cgtggcgagg tagctcctga tcgcatcgac gtcggcgcgc
     7381 gtgagcttcg tgtaccaggg atacggaaac gccggataga ggtgcttgcc ccccggggca
     7441 atgcctgcgt gcatcgcgcg gtagaaatcg tcggacgtcc agttgccgat ccccgtcgag
     7501 tcgtcgggcg tgatgttcgt cgaatagatg acgccgaacg gcgtgggtac cgcgcggccg
     7561 cctgcaaatg gctggccgcg ctcggcggta tggcacgctg cgcaatcgcc ggcctgcgcc
     7621 agatagcggc cgcgctcgat ctgcgcatgc gagccttcgc gtgcgaacag cgccggcgcg
     7681 acgccgagca ggccgccggt cgcgacggcc agcgcaacgg ccaccccaac gcgcgagcgt
     7741 cgcgtcttca tgccgacacc agcgcgccgg gcttcttcat gtactgcgtg cggatcgcat
     7801 tcgccgccca gtacgcgagc gcgccgacgg tgccggtcgg gttgtagccg gcgttttgcg
     7861 gaaacgcgca cgagccgacg acgaacaggt tcggcacgtc ccaggattgc aggtagcgat
     7921 tgaccacgct cgtgcgcgga tcggtgccca tgatcgcgcc gcccgtgttg tgcgtcgtct
     7981 ggtacgggac gatcgtgtag tggccctgac gcggattggc atccatgtgg cgtggcttca
     8041 tcgccttcgc gatcccggcc gccttctcgg tcaggaaggc cgacatcagg tgctcgttcg
     8101 ggtggaagtc gaacgtgagg cgaagcagcg gattgccgta ttcgtcgcg
//
LOCUS       DASSVX010000075        18039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1535607, whole genome shotgun sequence.
ACCESSION   DASSVX010000075 DASSVX010000000
VERSION     DASSVX010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 18039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18039
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535607"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="VFO53_03520"
     CDS             <1..106
                     /locus_tag="VFO53_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET8875375.1"
                     /translation="VRWHVAQRIVIAWVITLPAAALISAVVYFGTSRL"
     gene            complement(103..906)
                     /locus_tag="VFO53_03525"
     CDS             complement(103..906)
                     /locus_tag="VFO53_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018916947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET8875376.1"
                     /translation="MEMTVNRFKRSLGRRVMLGTWLMSGSTAAAEALGCLGFDFLVVD
                     TEHVPVDAPQALALLQAIAGTPAQPIVRLAWNDPVLVKRAMDMGAQSLMFPFVETADE
                     ARRAVAATRYPPAGSRGFAGMHRASRYGTVADFGRRANDEACVIIQIETLQAIARLEE
                     IAGVDGVDAIFAGPGDLSAACGHIGEVGHPEVQAHLLNAAATCRRLGKPCGTIAPHAD
                     MAKRFIDDGYSFVAVASDMAMMMRQAGEFLGQLRRKVDEPAAAGRTGGG"
     gene            complement(1010..1681)
                     /locus_tag="VFO53_03530"
     CDS             complement(1010..1681)
                     /locus_tag="VFO53_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003551363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8875377.1"
                     /translation="MTSVLITGCDTGLGVEFARQYAADGCRVVATCVDPATAHAVRAI
                     GADVRVVKLDVADLAAIDSLAGELRDEPIDILVSNAGIGRPHPPFGQTDYALWHRMLD
                     VNLIGPLRLAEAFVEHVAASSMKVMAFVSSRMGSIGLNLTGGSYAYRSSKAGLNAVVK
                     SLAVDLQPRHILVLALHPGWAKTEPGARVDVDASVAGMRAIIARCSRHETGSFFAYND
                     TLLPW"
     gene            complement(2128..3819)
                     /locus_tag="VFO53_03535"
     CDS             complement(2128..3819)
                     /locus_tag="VFO53_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017659016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HET8875378.1"
                     /translation="MSIKSDQWIRRMAQSHRMIEPFEPGQVREANGRRIVSYGTSSYG
                     YDIRCSDEFKIFTNINSTIVDPKQFDEGSFVDFKGDVCIIPPNSFALARTIEYFRIPR
                     SVLVCCLGKSTYARCFSGDTRVALVDGTSPTLENMARRANSGELFWGYSIGENGRVVV
                     SLLDAPRYIGRDALLDVELDSGDVIRATPDHDFMLRDGRMLAASALRPGQSLMPLYRD
                     LFRGYEMVYQPLNGHLVPTHRLADEWNLRGAFYADAPGTHRHHVDFDRRNNRPVNITR
                     MAASEHIRLHNAESYGAGFDARAHGESIRDALSRCASDPAWRDRFASLQAQRARDFWT
                     DPRYADIRVAVCKARRNPSETTRERHRQATMARYRDPNERIRQTGMSAAAWARDDGTR
                     RQRQAEVARAIRIRNEIDSECVRAALHQTGSIRGAARLLACDRSVFRRFPEVIAQFSG
                     RTMCNHKVVAVRERPGDHDVYCLTVPEAGNFALEAGVFVRNCGIIVNVTPLEPEWEGH
                     VTLEFSNTTPLPAKIYANEGVAQVIFFESDEVCETSYKDRGGKYQGQQGVTLPKM"
     gene            complement(3826..4260)
                     /locus_tag="VFO53_03540"
     CDS             complement(3826..4260)
                     /locus_tag="VFO53_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET8875379.1"
                     /translation="MRFWDTSALVPLLVAEQASEMVARAYREDRTVIVAWTTAIECVS
                     AFARKRRENQISEGLLARILQRLEDFRADWTVAEPTPQLVTLAQRAVARHGLHAADAI
                     QLASAMGSSSFEGAQPDIVCLDRRLTQAAMAEGFRILPPLLP"
     gene            complement(4260..4553)
                     /locus_tag="VFO53_03545"
     CDS             complement(4260..4553)
                     /locus_tag="VFO53_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HET8875380.1"
                     /translation="MKTATISEAKNHFSELLARVKQGETVLILDRDKPVARIVPIEVT
                     DRTDDERLADLERRGVIRRAAKPPRKTLPPPIELPEGVSVLEALLREREDARY"
     gene            complement(4644..5732)
                     /gene="apbC"
                     /locus_tag="VFO53_03550"
     CDS             complement(4644..5732)
                     /gene="apbC"
                     /locus_tag="VFO53_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HET8875381.1"
                     /translation="MAVTEQDVQQCLRQVVDPNLERDFVTAKSAKNIRVDGGNVSVDI
                     QLGYPARSQHESLKRLVHDALAKLPGVARVSVNVSQKTTSHAVQRGVKLVPGVRNIVA
                     VASGKGGVGKSTTAVNLALALAAEGASVGILDADIYGPSQPMMLGLAGRPESKDGKTL
                     EPMEAYGVQAMSIGFLIDTDTPMVWRGPMVTQALEQLLKDTNWRDVDFLVVDMPPGTG
                     DIALTLSQKVPVTGAVIVTTPQDIALIDARKGLKMFEKVGVPIVGVIENMSIHVCSNC
                     GHAEHIFGEGGAARMCADYNVPFLGSLPLDIRIREQADSGRPTVVADPDGAVAQAYKA
                     IARKVAISVAKRAEDFSAKFPSIVVQNT"
     gene            5883..7997
                     /gene="metG"
                     /locus_tag="VFO53_03555"
     CDS             5883..7997
                     /gene="metG"
                     /locus_tag="VFO53_03555"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET8875382.1"
                     /translation="MPASSSRTLFVTTALPYANGPFHIGHIMEYIQADIWVRFQRMQG
                     HVVHFVCADDTHGAPIMLAAEAEGITPEALIARVGESHREDWARYRISFDTTYSTHSP
                     ENVELAQSIFRALNRRGLIASRTISQFFDPVKGMFLPDRYIKGTCPKCGAKDQFGDAC
                     EVCGSTYAPTDLIEPYSTLTGAAPEMRESEHLFFRLSAPEVVAYLKEWTAAPAPGQAL
                     QPDVYNKVQEWLGDDGKRLDDWDISRDPPYFGIEIPDQPEGRPPKYFYVWLDAPIGYL
                     AALKKHFDSGAARALGETRSFDEFMAGAAVEQYHFIGKDIVYFHTLFWPAMLHFSGRK
                     TPTNVFVHGFITFEGHKMSKSRGTGISPQRYAELGLDPEWLRYYLAAKLNGKVEDIDF
                     SRDDFVARVNSDLIGKYVNIASRAAPFVTRHFEGRLATPAESATERVSRLARAQMDAE
                     AARANYDARESGKVLRDAMRIADRINEAFDAAQPWQLAKDPARRDLLQTVCSQALQGF
                     QALSVMLAPVLPGVASRVAREVFGLDREFVWQDAWQEPARVNAYRHLMTRIDPKKVAM
                     LVGDDAPPAAAPVPSSAVSTASAPARSAGGQITIDDFGKIDLRIARITAAETVEGADK
                     LVRLTLDIGEERSRTVFAGIKSSYAPETLVGRLTPMVANLAPRKMRFGVSEGMVLAAS
                     GEGPGIYLLTPDSGATPGMRVK"
     gene            7999..9996
                     /gene="hyfB"
                     /locus_tag="VFO53_03560"
     CDS             7999..9996
                     /gene="hyfB"
                     /locus_tag="VFO53_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HET8875383.1"
                     /translation="MIATPLGALAVAIAAWLAIGAVGLVRPRDLRVVQVLFAAGAAVA
                     LAVAGFAFAALAAVPEQLVLPLGLPDLPFHLRLDALSAFFLILLACASAAVSIFSSGY
                     FRAREGTAPGLIGFQYHVFIAAMALVLLADDAYLFMVAWETMALASFFLVTTDHRVPE
                     IRRAGFLYLLIAHVGAIAILLCFGVLQGGRGDYTFDAMRSASLTGVWPSIAFFLALFG
                     FGAKAGLLPLHVWLPEAHPAAPSPVSALMSGVMLKTAIYGILRVAFDLLHVQLWWWGA
                     IALALGLSTALFGVIYAAVQTDMKRLLAYSSIENIGVIVAAIGLAILFEANAKPLLAA
                     IALTAALYHALNHAFFKSLLFLVTGSVLHATHERSLGKLGGLIHRMPWVAWLALVGTL
                     AIAGLPPLNGFVSEWLLLQAFLFTPSLPQSFVNMLVPLAAAALVLAVALAAYVMVKFY
                     GVIFLGRPREPNLAYAQDAGPRERIALAMLALGCVLLGLFPVTVIGAIDSVNGLLLAT
                     TVGQAGQSSWLLLAPFATGRSSYSPLIVLVVIVAVVLITIQVVHRVWHGRVRRSSAWD
                     CGYPLQTPRMQDTAEGFGQPIRQVFEPFFRMERHLPSPFDARPRYEVRVSDHFWAWIY
                     VPIARAADGVSRLAGRIQQGRIGVYLTISFATLLALLFFVR"
     gene            10013..10960
                     /locus_tag="VFO53_03565"
     CDS             10013..10960
                     /locus_tag="VFO53_03565"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HET8875384.1"
                     /translation="MSGIGFLSQLLQVAIALALAPLLVGWVNQCRAWLSNKRAPSILV
                     PYRTIHKLFIKDAVVAENASPIFRLTPYVVFGAMTCAAAIVPSLATDAPFAIAADAIA
                     LVGLFALARVFIALAAMDIGTSFGSLGARREMFIGFLAEPALLMVLFTASLISGSTSL
                     PTIVDTLAHRELAIYPSLAFAGVAFTMVSLAENARIPIDNPSTHLELTMIHEAMTLEY
                     SARHLALLEWASALKLFNYSCIGLALFVPFGIADGANGLAMLVAAPALLAKLAAGGAL
                     LALIETLSAKLRLFRAPEFLGTAFLLAVLGMLVNLLLGS"
     gene            11055..11747
                     /locus_tag="VFO53_03570"
     CDS             11055..11747
                     /locus_tag="VFO53_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HET8875385.1"
                     /translation="MTSIPIGFQLINLLAAILLLLAFAMLAQRRVVSLIDLFAAQGFV
                     LALSTAVVAYTTGQPHLYQSTALTLLLKVFLLPWLLHRLVRKLDVRWEFEGLINIPTT
                     MLIGIVLVVFSFNLALPISQLANTVTRSTLGIAMASIMLSFLMMITRRKAIPQVVGFL
                     SMENGLFFAATSATYGMPMVVELGIALDVLVGMLILGVFFFQIREQFDSLDLKHFETL
                     REHGPGQDGAAE"
     gene            11747..13195
                     /locus_tag="VFO53_03575"
     CDS             11747..13195
                     /locus_tag="VFO53_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HET8875386.1"
                     /translation="MEIVVVLALPVIGAAVLAAIGHRREAALVNVVASLATLVAAAAL
                     TARVIENGPLLALGKQFLVDAFNVFLVALTALVGFTTAVFSRPYMMIEEQHGRLTPAR
                     LRLYHSMFQLFIFTMLLALLTNNVGILWVAMEAATLTTVLLVSLYRTPASLEAAWKYF
                     ILCGVGIAQALFGTILLYFAAEKLLGAGGGALLWTELDAVRADLEPTVLSLAFVFLLV
                     GYGTKVGLVPLHNWLPDAHAEGPTPVSAVLSGLLLNVALYAVVRCKVLVDGALPGAFA
                     KNLMIGFGLLSVVVAAFLLSRQRDIKRLFAYSSIEHMGIITFAFGLGGPIANFAGLLH
                     MTVHSLTKSAIFFAAGHAAQKTGTQIMDDIRGLVTISPVVGWGLALGTLAILGMPPFG
                     VFASEFLILTSAMREQPWATPFLLTALGVAFAAIFSKVQPMVFGETTATRLPVRPAML
                     PVFLHLSLVLMLGVWIPPFLAEWYRSAARLIG"
     gene            13192..14748
                     /locus_tag="VFO53_03580"
     CDS             13192..14748
                     /locus_tag="VFO53_03580"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET8875387.1"
                     /translation="MNVAPLGLAAAPLAAGVPAQRVPVSAGQLLTASRLAWENGGRLI
                     GLWGSDERDRHRGFCIRVALEDRDGLTVLEHTLPDANAHYPDLTSIFPAADRMQRATF
                     DLLGIAAESDDQRPWLWMASWPIDQFPLRRDFVPSPVWEPGQEEYDFVKVEGDGVHEI
                     PVGPVHAGIIEPGHFRFQIVGEKVLRLEQRLGYAHKGIEKRFEALPMLDAGRLAGRVS
                     GDSTVAYAWAYAQAVESLARTVPPPRASWLRALCLERERLASHLGDLGAIGNDGGFAF
                     GLAQFSRLREDVLRQNAAAFGHRLLMDVVVPGGVAHDLAWEHVHRMHDEADRIGREIV
                     ALRDIYDEHAGLQDRFRNCGRVAPELAARLGLLGLAGRASGRAHDLRCDFPCAPYDLL
                     SVRIASQTDGDVWARVAVRFDEAVETLRLIGAITAALLDPATSGDSRVALASIAPDRL
                     AAGYIEGWRGPVLVALESAPDGMIRRCHPQDPSWCNWPAIEHAIIGNIVPDFPLINKS
                     FNLSYSGHDL"
     gene            14755..15300
                     /locus_tag="VFO53_03585"
     CDS             14755..15300
                     /locus_tag="VFO53_03585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HET8875388.1"
                     /translation="MYQTLRRIIRIGIKTEDAPAPATGNVVVARLQERILHTLGRALT
                     IRHVDAGSCNACELEAQALSNPYYNLEGLGIRHVASPRHADALMVTGPVSKHMAIALK
                     RTYDAMPDPKLVIAVGECACTGGIFADSYATLGPLANVLPVDVAIPGCPPSPTALLQG
                     ILAAITNPGRTLHADPRAPVD"
     gene            15332..>18039
                     /locus_tag="VFO53_03590"
     CDS             15332..>18039
                     /locus_tag="VFO53_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET8875389.1"
                     /translation="MASTPRKSAPTPKPGSRRKSATLVTAPSTRDLLAQRETQLDLIN
                     LVQTGLARKLGFQAIVDLVGDRLRKVLRTPDLIITWFDEKSSLVHMLYAYEHGRRLNV
                     PPQAPKAGGIYETMRRTLAPIVLATAADYARVDYGAAPGTDPSRSMVCVPIFVGDRMA
                     GDISIENYERENAFGPDDVQILTTVAASLGGALDNAHLFDETQRLLKETEQRNAELAV
                     INSIQQGVAAELDFQKIVNLVGDKLREVLKTDEIGIRWLDHDKRITRYLYEYEHGVRL
                     EIAPTKYDDAGWERIATRREPRIVNTLAEALNVAILPGTDMAKSSLSVQIAGTNRVLG
                     AIVVESYEHEYAFGASDVRLLTTVASSMGVALENARLFDETQRLLKETEQRAAELAVI
                     NSIQEGISAKLDFQTIVDLVGDKLRDVFHTRDIGIRWFDHAKRIVHYLYDYEHGVRLT
                     IPAAPPATTPWETLVSRREPRVLHTAAEVAAGGVVPGTDTSLSVISVPIIGSDRVIGG
                     ITIESFEREYAFSDSDVRLLMTIASSMGVALENARLFDETQRLLKETEQRAAELAVIN
                     SVQQGMAAKLEFQSIVDLVGDKLRDVFATGDMGIRWHDQKTNLGHYLYAYEHGKRLTI
                     PPTPPTPGGLFETMVRTRQPMVLNCATDYATMPGGTIPGTDSSKSLLSVPIIAADRVL
                     GNITIENYERENAFGEAEIRLLTTIAASLGTALENARLFDETQRLFKESEQRAAELAV
                     INSVQQGLAAELDFRAIIDLVGDKIAEIFGSKDMSVALYDPQSNVTTMPYYLEHGERF
                     PIAPFQMGKGLTSHVIQTRRPLVINKDFMRRGVELGAGFIGDASAGNVGKSYVGVPIL
                     KGDEARGVVALYGVEEDAFDDAAVHLLATLANTMGVALENARLFDET"
BASE COUNT         2862 a         6118 c         5858 g         3201 t
ORIGIN      
        1 cgtgcgctgg cacgtcgctc agcggatcgt catcgcgtgg gtcatcacgc tgcccgcggc
       61 ggcactgatc agtgccgtcg tgtacttcgg gacgagccgc ctctagccgc cgcccgtgcg
      121 gccggccgcg gcgggctcgt cgaccttgcg ccgcaactgg ccgaggaact cgccggcctg
      181 gcgcatcatc atcgccatat cggacgcgac ggcgacgaac gaatagccgt cgtcgatgaa
      241 ccgttttgcc atgtcggcgt gcggcgcgat cgtgccgcac ggcttgccga ggcgccggca
      301 cgtcgccgcc gcattgagga ggtgcgcctg cacttccggg tgaccgacct cgccgatatg
      361 tccgcatgcc gcggacaggt cacccggtcc ggcgaagatc gcatccacgc cgtcgacacc
      421 cgcgatctcc tcgagtcgcg cgatcgcctg gagcgtttcg atctggatga tcacgcacgc
      481 ctcgtcgttc gcgcgccgtc cgaaatcggc gacggtgcca tagcggcttg cgcggtgcat
      541 gcccgcgaat ccgcgcgaac ctgccggagg atagcgggtg gcggcgaccg cgcggcgtgc
      601 ctcgtccgct gtctcgacga acggaaacat caggctctgc gcccccatgt ccatcgcccg
      661 cttgacgagc accggatcgt tccacgcgag gcgcacgatc ggctgtgccg gcgtgccggc
      721 gatcgcctgc agcagcgcga gcgcctgcgg cgcatcgacg ggaacgtgct cggtgtcgac
      781 gacgagaaaa tcgaatccga ggcagccgag cgcctcggcc gcggccgtgc tccccgacat
      841 gagccaggtt ccgagcatga cgcggcgtcc gagcgaacgc ttgaagcggt tgacggtcat
      901 ttccatgtgg cgctccgcgg gtttaccgct cgatgaatct cgggttcggg caccggtgcg
      961 cgcgaatcaa ggaaaccgaa atcgcagcct tccggcgcct ggtcttttct caccagggca
     1021 gcagcgtgtc gttgtaggcg aagaagctgc cggtctcgtg acggctgcag cgggcaatga
     1081 tcgcgcgcat gccggcgacg cttgcgtcga cgtcgacgcg cgcaccgggc tcggttttcg
     1141 cccagccggg gtgcagcgca agcacgagga tgtgtcgcgg ctggaggtcg accgcgaggc
     1201 ttttcacgac ggcgttgagg cccgccttgc tcgagcgata cgcataggag ccgcccgtca
     1261 ggttgagccc gatgctgccc atgcggctcg aaacgaacgc catcaccttc atcgagctcg
     1321 cggcgacgtg ctcgacgaac gcctcggcaa ggcgcaacgg accgatcagg ttgacgtcga
     1381 gcatccgatg ccacagcgcg tagtccgtct gcccgaacgg cggatgcggc cgtccgatac
     1441 cggcgttgct cacgagaatg tcgatcggct catcgcgcag ctcgccggcg agcgaatcga
     1501 tcgcggcgag atcggcgacg tcgagcttca cgacgcgaac gtccgcgccg atcgcgcgca
     1561 ccgcgtgcgc ggtcgcaggg tcgacgcacg tcgcaacgac ccggcagccg tccgccgcgt
     1621 attgccgggc gaactcgacg cccaatcccg tatcgcaacc cgtgatcaaa acgcttgtca
     1681 tcggcgccgg ctcctcgctt cgatcttatc gcatccttcc gcgttcgccc ggcaaagcga
     1741 ccgcaaccgc tcgccacaac gcattcccac aggagttgtc atgtcccgct tcatgcgatg
     1801 caacggcttg tcggctctcg tgcccgtcgc cgccatcggt gcggcgcacg ccgccggcca
     1861 aagcgcagac gtcgccattc gcggacgggt cgatgccgtg gaaatgctcg ggtcggagca
     1921 gtacgtgcat accgtcgtcg acggtggggc ggtcaccgcg cgcgtgccga gaagccagcg
     1981 cgtcagggcc gaggaccgcg tgacgctgaa ggccgaggcc cacgacctgc accttttcga
     2041 gcgcgccacc ggccgggcgt tgcgccaagg cttgttcaca ctttgccggg gcacgcgaac
     2101 cgcgctgccg cgcgagtcac gctgcgatta catcttcggt agcgtgacgc cctgttgccc
     2161 ctggtacttg ccgccgcgat ccttgtacga cgtttcgcaa acttcgtcgg actcgaagaa
     2221 gatcacctgc gcgacgccct cgttggcgta gatcttcgcc ggcagcggcg tcgtgttcga
     2281 gaactcgagc gtgacatgcc cctcccattc gggctcgagg ggcgttacgt tcactatgat
     2341 gccgcaattg cggacgaaaa cgccggcttc cagagcgaag ttgccagctt caggtaccgt
     2401 caggcagtac acgtcgtggt cgccgggccg ttcgcgcaca gcgactacct tgtgattgca
     2461 cattgtgcgt ccgctgaatt gcgcgataac ttccggaaag cggcggaaca ccgaacggtc
     2521 gcacgcgaga agtctcgcgg cacctcgaat ggaacccgtt tggtgtagcg ctgcccgcac
     2581 gcactcactg tcaatttcgt ttcgaatccg gattgctcgc gccacctcgg cttgccgttg
     2641 gcgacgcgtg ccgtcgtcgc gcgcccatgc cgccgcgctc atacctgtct gtctgatccg
     2701 ctcgttcggg tcgcggtagc gggccatcgt cgcctgccgg tggcgctcac gcgttgtttc
     2761 gcttggattg cgacgagctt tacagaccgc aacacgaatg tcggcatacc gcggatcagt
     2821 ccagaagtcg cgagcccgtt gcgcctgcag cgatgcaaag cgatcgcgcc aagcgggatc
     2881 gctcgcgcag cgtgagagcg catcgcggat tgactcgccg tgcgctcgcg cgtcaaatcc
     2941 ggcaccgtaa ctttccgcgt tgtgcaggcg aatgtgctcg gaggcggcca ttcgcgtgat
     3001 gttcaccggc cgattgttgc ggcggtcgaa atccacatga tggcggtggg ttccgggtgc
     3061 atccgcgtag aaggcccctc gaagattcca ctcgtccgcg agacgatgcg tcggcacgag
     3121 gtgcccgttc aacggctgat aaaccatctc gtaaccgcgg aaaaggtcgc gatacagcgg
     3181 catcagcgac tgcccgggtc gcagcgccga tgccgcaagc atgcgtccgt cacgcagcat
     3241 gaaatcgtgg tccggcgtcg cgcggatcac atcgccgcta tcgagttcca cgtccagcag
     3301 cgcgtcacgt ccgatgtagc gtggagcgtc gaggagcgac accacgacgc ggccgttctc
     3361 gccaatgctg tatccccaga aaagctcgcc ggaattcgcg cgccgcgcca tgttttccag
     3421 cgtcggtgac gtaccgtcga ccagcgccac gcgggtgtct ccgctgaagc atcttgcgta
     3481 cgtcgattta ccgagacagc aaaccagcac gctgcgcgga atccggaagt actcgatcgt
     3541 gcgcgccagc gcgaacgaat tgggcgggat gatgcaaacg tcgcccttga aatcgacgaa
     3601 cgatccttcg tcgaattgct tcgggtcgac gatcgtcgag ttgatgttcg tgaagatctt
     3661 gaattcgtcc gagcagcgaa tgtcgtagcc gtagctcgac gtgccgtacg acacgatcct
     3721 gcggccgttc gcctcgcgca cctggccggg ctcgaacggc tcgatcatcc ggtgcgattg
     3781 cgccatccgg cggatccatt ggtccgattt gatggacatg tcgcgtcagg gcagcaatgg
     3841 cggcaggatg cggaatcctt ccgccattgc cgcttgggtc agccgccggt ccaggcacac
     3901 gatgtcgggt tgcgcgcctt cgaacgagct gctgcccatc gcgctcgcga gctggatcgc
     3961 gtccgcagcg tgcaggccat ggcgggcaac ggcgcgttgc gcgagcgtga cgagttgtgg
     4021 cgtgggttcc gccacggtcc agtcggcgcg aaaatcttcg agccgctgga gaatcctggc
     4081 gagaagtccc tcggaaatct ggttctcgcg acgcttgcgg gcaaaggcgg aaacgcattc
     4141 gatggcggtc gtccacgcca cgatgacggt ccggtcttcc cggtacgctc gcgcgaccat
     4201 ttcgctggcc tgttccgcaa caaggagcgg gacgagcgcc gacgtgtccc agaaacgcat
     4261 caatagcgcg cgtcttcccg ctctcggaga agcgcttcga gaacgctgac gccctcaggc
     4321 aattcgatcg gcggcggaag cgtcttgcgc ggtggcttgg cggcccgccg gatgacgcca
     4381 cgccgctcga gatcggcgag acgctcgtcg tccgtgcggt ccgtcacctc gatcggcacg
     4441 atgcgcgcaa cgggcttgtc gcgatccagg atcagcacgg tctcgccctg cttcaccctc
     4501 gccaacagct cggaaaagtg attcttggcc tcggaaatgg tcgccgtctt catctggcca
     4561 tgttagccat gtccgtgcgg agcgtcaatc cagctttcgg ccgagctcca tgggtgtgcg
     4621 gcttgccgga gaccgtgcgc ccgctacgtg ttctgcacga cgatgctcgg gaatttagcc
     4681 gagaaatcct cggcccgctt ggcaaccgag atcgcgacct tccgcgcgat cgccttgtat
     4741 gcctgcgcca cggcgccatc gggatcggca acgacggtcg ggcggcccga atcggcctgc
     4801 tcgcggatgc ggatgtcgag cggcagactg ccgaggaacg gcacgttgta gtcggcgcac
     4861 atccgggcag cgccgccctc gccgaagatg tgctcggcgt ggccgcagtt cgagcagacg
     4921 tggatcgaca tgttctcgat cacgccgacg atcggcacgc cgaccttctc gaacatcttg
     4981 agtcccttgc gcgcgtcgat cagcgcgatg tcctgcggcg tcgtgacgat caccgcgccg
     5041 gtgaccggca ccttctgcga cagcgtcagc gcgatgtcgc cggtaccggg cggcatgtcg
     5101 acgacgagga aatcgacgtc gcgccagttc gtgtccttca agagctgctc gagcgcctgc
     5161 gtgaccatcg ggccgcgcca gaccatcggc gtgtcggtgt cgatcaggaa gccgatcgac
     5221 atcgcctgca cgccgtacgc ctccatcggc tcgagcgtct tgccgtcctt cgactcgggt
     5281 cgcccggcga gaccgagcat catcggctgc gacggaccgt agatgtccgc atcgagtatg
     5341 ccgacactcg cgccttccgc ggcaagtgcg agcgcgaggt tgacggcggt cgtcgacttg
     5401 ccgacgccgc ccttgccgct cgcgacggcg acgatgttgc gcacgccggg aacgagcttg
     5461 acgccgcgct gaaccgcgtg cgacgtcgtc ttctgcgaca cgttgacgct cacccgcgcc
     5521 acgccgggca gcttcgcgag cgcgtcgtgc accaggcgct tcagcgactc gtgctggctg
     5581 cgggccggat acccgagctg gatgtcgaca ctcacgttgc cgccgtcgac gcgaatgttc
     5641 ttcgccgact tggcggtcac gaaatcgcgt tcgagattgg gatcgacgac ctgccgcagg
     5701 cactgctgca cgtcttgctc tgttaccgcc attgtcgtcc tctgctctcc gatcgcccgc
     5761 gaacgtcatg tgtcacgcgc gaaaacctgc attttagcct ctcgcatcgc cgcggccggc
     5821 gcaaacatgc ggtttgctag aattcgcgat tcgcaagcct cccctccccg ctcaaaatcc
     5881 gcatgcccgc cagctcgtcc cgcaccttgt tcgtgacgac ggcgctcccg tatgcgaacg
     5941 gcccgttcca tatcggccac atcatggagt acatccaggc cgatatctgg gtgcgcttcc
     6001 agcggatgca ggggcacgtc gtgcacttcg tgtgtgcgga cgacacgcac ggcgcgccga
     6061 tcatgctggc cgccgaggcc gagggcatca cgcccgaggc gctgatcgcg cgcgtcggcg
     6121 aatcgcaccg cgaggactgg gcgcgctacc gcatcagctt cgacacgacc tactcgacgc
     6181 attcgcccga gaacgtcgag ctcgcgcagt cgatcttccg cgcgctcaac cgccgcggcc
     6241 tcatcgcaag ccgcacgatc tcgcagttct tcgacccggt gaagggcatg ttcctgcccg
     6301 accgttacat caagggaacg tgtcccaagt gcggcgcgaa ggaccagttc ggcgacgcct
     6361 gcgaagtctg tggctcgacg tacgcgccca ccgacctaat cgagccgtat tcgacgctca
     6421 cgggtgcggc acccgaaatg cgcgaatcgg agcatctgtt cttccggctg tcggcgcccg
     6481 aggtcgtcgc gtacctcaag gaatggacgg cggcgccggc gcccgggcag gcgctgcagc
     6541 ccgacgttta caacaaggtg caggaatggc tcggcgatga cggcaagcgg ctcgacgact
     6601 gggacatctc gcgcgacccg ccgtacttcg gcatcgagat tcccgatcag cccgaaggga
     6661 ggccgccgaa gtatttctac gtgtggctcg acgcgccgat cggctatctc gctgcgctga
     6721 agaagcattt cgattccggc gccgcgcgcg cgctcggcga gacgcgcagc ttcgacgaat
     6781 tcatggccgg cgcggcggtc gagcagtacc acttcatcgg caaggacatc gtctatttcc
     6841 atacgctgtt ctggcccgcg atgctgcact tttcgggtcg caagacgccg acgaacgtat
     6901 tcgtgcacgg cttcatcacg ttcgaaggcc acaagatgtc gaagtcgcgc ggcaccggca
     6961 tctcgccgca gcgctacgcc gagctcggcc tcgatcccga gtggctgcgc tactacctcg
     7021 cggcgaagct caacgggaag gtcgaggaca tcgacttcag ccgcgacgat ttcgtcgcac
     7081 gcgtcaacag cgacctgatc ggcaagtacg tgaacatcgc gagccgcgcg gcgccgttcg
     7141 ttacgcgcca cttcgagggc cggctcgcga cgccggccga atccgcgacc gagcgcgtca
     7201 gccggctggc acgcgcgcag atggatgccg aagcggcacg cgcgaactac gacgcgcgcg
     7261 aatccggcaa ggtgttgcgc gacgccatgc gcatcgctga tcggatcaac gaggcgttcg
     7321 acgcggcgca gccctggcag ctagcgaagg atccggccag gcgcgacctg ctgcagacgg
     7381 tctgctcaca ggcactgcag ggattccagg cgttgagcgt gatgctcgcg ccggtgctgc
     7441 ccggcgtcgc ctcgcgcgtc gcgcgtgagg tgttcggtct cgaccgcgag ttcgtgtggc
     7501 aggacgcgtg gcaggaaccg gcgcgcgtca acgcctacag gcacctgatg acgcgcatcg
     7561 atccgaagaa ggtcgcgatg ctcgtcggcg acgacgcgcc accggcggca gcaccggtac
     7621 cctcgtccgc cgtgagtacg gcgtcggcac ctgcacggtc cgccggcggg cagatcacga
     7681 tcgacgattt cggcaagatc gatctgcgca tcgcgcgcat caccgccgcc gaaacggtcg
     7741 aaggcgcgga caagctcgtc aggctgacgc tcgacatcgg cgaagagcga tcgcgcaccg
     7801 ttttcgccgg gatcaagtct tcctatgcgc ccgagacgct cgtcggacgc ctcacgccga
     7861 tggtggccaa tctcgccccg cgcaagatgc gcttcggcgt gtccgaaggc atggtgctcg
     7921 cggcgtccgg cgaaggaccg ggcatttatc tgctgacccc cgattccggc gcgacgccgg
     7981 gcatgcgcgt caagtaacgt gattgccacg ccgctaggcg cgctcgccgt cgccatcgcg
     8041 gcgtggctcg cgatcggtgc ggtcggtctt gtgcggccgc gcgatctgcg cgtcgtgcag
     8101 gtgctgttcg ccgccggtgc agccgtggcg ctcgcggtgg ccggctttgc attcgccgcc
     8161 ctcgccgcgg tgcccgagca actggtgctg ccgctcggct tgcccgatct gccgtttcat
     8221 ctgcggctcg atgcgctgtc cgcgttcttc ctgatcctcc ttgcgtgcgc cagcgccgcg
     8281 gtgtcgatct tttcgtcagg ctacttccgc gcgagagaag gcaccgcgcc cgggctcatc
     8341 ggcttccagt atcacgtgtt catcgcggcg atggcgctcg ttctcctcgc cgacgatgcg
     8401 taccttttca tggtggcatg ggaaacgatg gcgctcgcgt cgttcttcct cgtgacaacc
     8461 gaccaccggg ttcccgagat acgccgcgcc ggcttcctgt acctgttgat cgcgcacgtc
     8521 ggcgcgatcg cgatcctgct ctgcttcggc gtgctgcagg gcggccgcgg cgactacacg
     8581 ttcgacgcga tgcgcagcgc gtcgctcacc ggcgtgtggc cgagcatcgc gtttttcctg
     8641 gcgcttttcg gcttcggggc aaaagccggc ctgcttccgc tgcacgtctg gctccccgaa
     8701 gcgcacccgg ccgcgccgtc gccggtgtcg gcgctgatga gcggcgtgat gctcaagacg
     8761 gcgatctacg ggatattgcg cgtcgccttc gatctgctgc acgtgcaact gtggtggtgg
     8821 ggcgcgatcg cgctcgcgct cgggctctca acggcgctgt tcggcgtcat ctacgcggcg
     8881 gtgcagaccg acatgaagcg cctgctcgcg tattcgtcga tcgagaacat cggcgtcatc
     8941 gtcgccgcga tcgggcttgc gatcctcttc gaggcgaacg caaagccgct gctcgccgcc
     9001 atcgcgctca ccgcggcgct gtaccacgcg ctcaatcacg cgttcttcaa gagcctgctg
     9061 ttcctcgtca cgggctcggt gctgcatgca acgcacgagc gcagcctcgg caagctgggc
     9121 ggcctcatcc accggatgcc gtgggtcgcc tggcttgcgc tcgtcggcac gctcgccatc
     9181 gcgggactgc cgccgttgaa cggattcgtg tccgagtggc tgctgctgca ggcgttcctg
     9241 ttcacgccga gcctgccgca atcgttcgtc aacatgctgg tgcctctggc ggcggccgcg
     9301 ctggtgcttg ccgttgcgct cgccgcgtac gtgatggtca agttctacgg cgtgatcttc
     9361 ctcgggcgtc cgcgcgagcc gaatctcgcg tatgcacagg atgcaggccc gcgcgaacgc
     9421 atcgcgctgg cgatgctggc gttgggctgc gtgctgctgg ggctctttcc ggtgaccgtc
     9481 atcggcgcga tcgacagcgt caacgggctg cttcttgcga cgaccgtcgg ccaggccggc
     9541 caatcgagtt ggctgctgct cgcgccattc gccaccgggc gttcgagcta cagcccgctg
     9601 atcgtgctcg tcgtgatcgt cgccgtcgtg ctgatcacga tccaggtcgt gcaccgcgtg
     9661 tggcacggcc gcgtgcgccg gtccagtgcg tgggattgcg gctatccgct gcagacgccg
     9721 cggatgcagg acaccgcgga gggcttcggc cagccgatac gccaggtgtt cgagccgttc
     9781 ttccggatgg agcggcacct gccgtcgccg ttcgacgcgc ggccgcgcta cgaggtgcgc
     9841 gtcagcgacc atttctgggc gtggatctat gtgccgattg cgcgcgcggc ggacggggtg
     9901 tcgcgcctcg ccggccgcat ccagcaggga cgcatcggcg tctacctgac gatcagcttc
     9961 gcgacgctgc tcgcgctgct gttcttcgtt cgatgaaagc acaaacgctg cgatgagcgg
    10021 catcggcttc ctgtcgcagc tgctgcaggt ggcgattgcg ctcgccctcg cgccgctgct
    10081 cgtcggatgg gtgaaccagt gccgggcgtg gctatcgaac aagcgcgcgc cgtccatcct
    10141 cgttccgtac cggacgatcc acaagctgtt catcaaggac gcggtggtcg ccgagaacgc
    10201 gtcgccgatc ttccgcctga cgccgtacgt cgtgttcggc gcgatgacgt gcgccgcggc
    10261 catcgtgccg tcgctcgcga ccgacgcgcc gttcgcgatc gcggccgacg cgattgccct
    10321 cgtcgggctc ttcgcacttg cgcgcgtgtt catcgccctc gccgccatgg acatcggcac
    10381 gtcgttcggc tcgctcggtg cgcgccgcga gatgttcatc ggcttcctcg ccgagccggc
    10441 actgctgatg gtgctgttca ccgcgtcgct gatttccggc tcgacgtcgc tgccgacgat
    10501 cgtcgacacg cttgcgcatc gcgagctcgc aatctatccg agcctcgcat tcgccggcgt
    10561 ggcattcacg atggtgtccc ttgcagagaa cgcgcgcatt ccgatcgaca acccgtcgac
    10621 gcacctcgag ctcacgatga tccacgaggc gatgaccctc gaatactcgg cgcgccacct
    10681 ggcactgctc gagtgggcct cggcgctcaa gcttttcaac tactcgtgca tcggtctcgc
    10741 gctgttcgta ccgttcggca tcgccgacgg cgcgaacggg ctcgcgatgc tcgtcgccgc
    10801 accggcgctg ctcgcgaagc tcgccgccgg cggtgcgctg ctggcgctga tcgagacgct
    10861 gtcggcgaag ctgcggctct tccgcgcgcc ggagtttctc ggcacggcat tcctcctggc
    10921 cgtgctcggc atgctcgtca acctgctgct cggcagttga tggtcccccc acgcttgcgg
    10981 ttttcaccgc tgcgctgccc ccggaggggg cgcgattcgc gccttgggac ggcccggcgg
    11041 cgctcatgga cgcgatgacc tcgatcccga tcggcttcca gctcatcaac ctgctcgcag
    11101 cgatcctgct gctgctcgcg ttcgcgatgc ttgcgcagcg gcgtgtcgtg tcgctgatcg
    11161 acctcttcgc cgcgcaggga ttcgtgctgg cgctgtccac ggccgtggtc gcttacacga
    11221 cggggcagcc gcacctgtac cagtcgacgg cattgacact gctcctcaag gtgttcctgc
    11281 tgccgtggct gctgcaccgg ctggtgcgaa agctcgacgt gcgctgggaa ttcgagggcc
    11341 tgatcaacat tccgacgacg atgctgatcg gcatcgtgct cgtggtcttc tcgttcaacc
    11401 tggcgctgcc gatctcgcag cttgcgaaca cggtcacgcg ttcgacgctc ggcatcgcga
    11461 tggccagcat catgctttcg ttcctgatga tgatcacgcg gcgcaaggcc atcccgcagg
    11521 tggtcggctt cctgtcgatg gagaacggcc tgttcttcgc ggcgacgagc gccacgtacg
    11581 ggatgccgat ggtcgtcgag ctcggcatcg cgctcgacgt gctcgtcggc atgctgatcc
    11641 tcggcgtgtt cttcttccag attcgcgagc agttcgacag cctcgatctc aagcatttcg
    11701 agacgctgcg cgaacacggc ccgggccagg acggagcggc ggagtgatcg agatcgtcgt
    11761 cgtcctggca ctccccgtga tcggcgccgc cgttctcgcc gccatcggcc atcggcgcga
    11821 ggcggcgctc gtgaacgtcg tcgcaagcct cgccacgctc gtcgccgccg ccgcgctgac
    11881 cgcgcgcgtg atcgagaacg gcccgctgct ggcactcggc aagcagttcc tcgtcgacgc
    11941 gttcaacgtg ttcctggtcg cgctcaccgc gctcgtcggc ttcacgaccg ccgtcttctc
    12001 gcgtccgtac atgatgatcg aggagcagca cggccgcctg acgcccgcgc gcctgcgtct
    12061 ttatcacagc atgttccagc tcttcatctt cacgatgctg ctggcgctgc tcaccaacaa
    12121 cgtgggcatc ctgtgggtcg cgatggaggc cgcgacgctg acgacggtgc tgctcgtgtc
    12181 gctctatcgc acgccggcga gcctcgaggc cgcatggaag tacttcatcc tgtgcggcgt
    12241 cggcattgca caggcgctgt tcggcacgat cctgctctat ttcgccgcgg aaaagctgct
    12301 cggcgcaggc ggcggcgcgc tgctgtggac cgagctcgac gcggtgcgtg ccgatctcga
    12361 gcccacggtg ctgtcgctcg cgttcgtctt cctgctggtg ggctacggca cgaaggtcgg
    12421 gctggtgccg ctgcacaact ggcttcccga tgcgcacgcc gaaggcccga cacccgtttc
    12481 cgcggtgctg tcggggctgc tgctcaacgt cgcgctctac gcggtcgtgc gctgcaaggt
    12541 gctggtcgac ggcgcgctgc ccggcgcatt cgcgaagaac ctgatgatcg gcttcggatt
    12601 gctttcggtc gtggtcgccg cattcctgct gtcacgccaa cgcgacatca agcggctctt
    12661 cgcgtactcg tcgatcgagc acatgggcat catcacgttc gcgttcgggt tgggcggccc
    12721 gatcgcgaac ttcgccggcc tcctgcacat gaccgtgcat tcgctcacga aatcggcgat
    12781 cttcttcgcc gccgggcacg cggcgcagaa gacgggcacg cagatcatgg acgacatccg
    12841 cggcctcgtc acgatcagtc ccgttgtcgg ctgggggctc gcgctcggca cgctggcaat
    12901 cctcggcatg ccgccgttcg gcgtattcgc gagcgagttc ctgatcctga cgagcgcgat
    12961 gcgcgagcag ccgtgggcga cgccgttcct gctgaccgca ctcggcgtcg cgttcgccgc
    13021 catcttctcg aaggtgcagc cgatggtctt cggcgagacg accgcaaccc ggctgcccgt
    13081 tcggccggcg atgctgcccg tcttcctgca cctgtcgctc gtgctgatgc tcggcgtgtg
    13141 gatcccgccg tttctcgccg agtggtaccg cagcgcggca aggctgatcg gatgaacgtg
    13201 gcaccgctcg ggctcgccgc cgcaccgctc gccgcggggg ttcccgcgca gcgcgtgccg
    13261 gtcagcgcgg gacagttgct caccgcgtcg cggcttgcct gggaaaacgg cggccggctc
    13321 atcggcctgt ggggcagcga cgagcgcgac cggcaccgcg gcttctgcat tcgcgtcgcg
    13381 ctcgaggacc gcgacggact cacggtgctc gagcacacgc tgcccgatgc caacgcgcac
    13441 tatcccgatc tcacgtcgat ctttccggcg gccgaccgga tgcagcgcgc gacgttcgat
    13501 ctactcggca tcgcggccga atccgacgac cagcggccgt ggctatggat ggcgagctgg
    13561 ccgatcgacc agtttccact gcgccgcgat ttcgtcccgt caccggtgtg ggaacccggc
    13621 caggaggaat acgacttcgt gaaagtcgaa ggcgacggcg ttcacgaaat acccgtcggt
    13681 cccgtgcatg cgggcatcat cgagcccgga catttccgct tccagatcgt cggcgaaaag
    13741 gtgctacggc tcgagcagcg gctcggctac gcgcacaagg gaatcgaaaa gcgcttcgag
    13801 gcgctgccga tgctcgacgc cggacggctc gccggccgcg tgtcgggcga cagcaccgtc
    13861 gcctacgcgt gggcgtatgc gcaggccgtc gaaagcctcg cgcgcaccgt gccgccaccg
    13921 cgcgcatcgt ggctgcgcgc gctgtgcctc gagcgcgagc gcctcgcgag ccatctgggc
    13981 gatctcggcg cgatcggcaa cgacggcggg ttcgcgttcg gcctcgcgca gttctcgcgc
    14041 ttgcgcgaag acgtgctgcg gcagaacgcg gcagcgttcg ggcatcggct gctgatggac
    14101 gtcgtcgtgc cgggcggcgt ggcacacgat ctcgcctggg agcacgtgca tcgcatgcac
    14161 gacgaggccg atcgcatcgg ccgcgagatc gtcgcgttgc gcgacatcta cgacgagcat
    14221 gcgggactgc aggaccggtt tcgcaactgc ggacgcgtgg cacccgagct cgcggcgagg
    14281 ctcggcctcc tcggcctcgc cgggcgggcg agcggacggg cgcacgactt gcgttgcgat
    14341 ttcccgtgcg cgccgtacga tctgctttcg gttcgcatcg cgagccagac cgacggcgac
    14401 gtgtgggcgc gcgtcgccgt gcgcttcgac gaggccgtcg agacgctgcg cctcatcggg
    14461 gcgatcaccg cggcactgct cgaccccgcg acgtcgggcg acagccgggt ggcgcttgcg
    14521 agcatcgcgc ccgaccggct tgccgccggt tacatcgagg gctggcgcgg tccggtgctc
    14581 gttgcgctcg aaagcgcgcc ggacgggatg atccgccgct gccatccgca ggacccgtcg
    14641 tggtgcaact ggcccgcgat cgagcatgcg atcatcggca acatcgttcc cgactttccg
    14701 ctgatcaaca aatcgttcaa cctgtcgtac agcgggcacg acctgtaacg cgccatgtac
    14761 cagacactgc gccggatcat ccggatcggc atcaaaaccg aggacgctcc cgcaccggcg
    14821 accgggaatg tcgtcgtcgc gcgtctgcag gagcgaatcc tgcacacgct cggacgcgcg
    14881 ctcacgatca ggcacgtcga cgccggctcg tgcaacgcgt gcgagctcga ggcgcaggcg
    14941 ctgtccaacc cgtactacaa cctcgaaggc ctcggcattc gtcacgtcgc gagcccgcgt
    15001 cacgccgacg cgctgatggt caccggaccg gtaagcaagc acatggcgat cgcgctcaag
    15061 cgcacctacg acgcgatgcc cgacccgaag ctcgtcatcg ccgtcggcga atgcgcatgc
    15121 accggcggca tctttgccga cagctacgcg acgctcgggc cgctcgcgaa cgtgctgccg
    15181 gtcgacgtcg cgatccccgg ctgcccgccc tcgccgaccg cactgttgca gggcattctc
    15241 gcggccatca cgaatcctgg ccgcacactc cacgccgacc ctcgcgcgcc tgtagactag
    15301 cgcccacctc aaatagggtg acgagaagcg catggctagc acgccgagaa aatccgcccc
    15361 gacgccaaag ccgggctcgc gacgcaagtc agccacgctg gtaaccgcgc catcgacgcg
    15421 cgacctcctc gcacagcgcg agacgcagct cgacctcatc aacctggtcc agacggggct
    15481 cgcgaggaag cttggcttcc aggcgatcgt cgatctcgtc ggcgacaggc tgcgcaaggt
    15541 cctacgcacg ccggacctga tcatcacctg gttcgacgag aagtcgagcc tggtccacat
    15601 gctgtacgcc tacgagcatg ggcgccgctt gaacgttccg ccacaagccc cgaaagcggg
    15661 cggcatctac gagacgatgc gccggacgct tgcgccgatc gtcctcgcga ccgcggccga
    15721 ctacgcgcga gtggactacg gcgctgcgcc gggaaccgac ccgagccgat ccatggtgtg
    15781 cgtgcccatc ttcgtcggtg accggatggc cggcgacatc agcatcgaga attacgagcg
    15841 cgagaacgcg ttcgggcccg acgacgttca gatcctcacc acggttgccg cgtcgctcgg
    15901 cggcgcactc gacaacgccc acctgttcga cgagacgcag cgcctgctga aggaaaccga
    15961 gcagcgcaat gccgagctcg ccgtcatcaa cagcatccag cagggggtgg cggcggagct
    16021 cgacttccag aagatcgtca acctcgtcgg cgacaagctg cgcgaggtcc tgaagaccga
    16081 cgagatcggc atccgctggc tcgaccacga caagcggatc acgcgctacc tgtacgaata
    16141 cgagcacggg gtccggctcg aaattgctcc gacgaagtac gacgacgctg gatgggagcg
    16201 catcgccacc cggcgcgagc cacgcatcgt caacacgctc gccgaagcgc tcaacgtggc
    16261 catcctgcca ggcaccgaca tggcgaagtc gagcctctcg gtgcagatcg cgggcacgaa
    16321 ccgcgtgctc ggcgccatcg tcgtcgagag ctacgagcac gaatacgcgt tcggcgcgtc
    16381 cgacgtgcgc ctgctcacga ccgttgcctc gagcatgggc gtcgcgctgg agaacgcgcg
    16441 cctcttcgat gaaacgcagc ggctgttgaa ggaaaccgag cagcgggcgg ccgagctcgc
    16501 cgtgatcaac agcatccagg agggaatctc ggcgaagctc gatttccaga cgatcgtcga
    16561 tctcgtcggc gacaagctgc gcgatgtgtt ccatacccgc gatatcggca tccgctggtt
    16621 cgaccacgcg aagcggatcg ttcattacct ctacgattac gagcacgggg tgcgcctgac
    16681 gatcccggcc gcgccgccgg cgacgacgcc ctgggaaacg ctcgtttcgc ggcgcgagcc
    16741 gcgcgtgttg catacggcgg ccgaggtcgc ggccggtgga gtcgttccgg gcacggatac
    16801 gtcgctgtcg gtcatttccg tgccgatcat tggcagcgac cgcgtcatcg gcggcatcac
    16861 gatcgagagc ttcgagcgcg agtacgcatt cagcgattcc gatgtccgcc tgctcatgac
    16921 gattgcttcc agcatgggtg tagcgctcga gaacgcgcgc ctgttcgacg agacgcagcg
    16981 tctgctgaag gagaccgagc agcgcgcggc cgagctcgcg gtgatcaaca gcgtccagca
    17041 gggaatggca gcgaagctcg agttccagag catcgtcgat ctcgtcggcg acaagttgcg
    17101 cgacgtgttc gccaccggtg acatggggat tcgctggcac gaccagaaga cgaatctcgg
    17161 ccactacctt tacgcctacg agcatggcaa gcggctgacg attcctccta ccccgcccac
    17221 gccgggcggc ctattcgaga cgatggtgcg cacgcggcag ccgatggtgc tgaactgcgc
    17281 gaccgactat gcgacgatgc cgggtggcac gatcccgggc accgacagca gcaagtcact
    17341 gctctccgta ccgatcatcg ctgccgatcg cgtgctcggg aacatcacga tcgaaaatta
    17401 cgagcgcgag aacgcgttcg gcgaggccga gattcgtctg ttgacgacga tcgcggcatc
    17461 gctcggtacg gcactcgaga acgcgcgcct tttcgacgag acgcagcggc tgttcaagga
    17521 aagcgagcag cgcgccgccg agctcgcggt gatcaacagc gtccagcagg ggcttgcggc
    17581 ggagctcgat ttccgggcga tcatcgacct cgtaggcgac aagatcgccg agattttcgg
    17641 ctcgaaggac atgtcggtcg cgctgtacga cccgcaaagc aacgtgacga cgatgccgta
    17701 ttacctcgag catggcgagc ggtttccgat cgcaccgttt cagatgggca agggattgac
    17761 ctcgcatgtc atccagacgc ggcgtccgct ggtgatcaac aaggatttca tgcggcgagg
    17821 cgtcgaactg ggcgcaggtt tcatcggcga tgcgagtgca ggaaacgttg gaaaaagcta
    17881 cgtcggcgtc cccatcctga aaggcgacga ggcacgcggc gtcgtcgcgc tctacggcgt
    17941 cgaggaagac gcgttcgacg acgccgccgt gcacttgctc gcgacgctgg caaacacgat
    18001 gggcgtcgcc ctcgagaacg cgcggctctt cgacgagac
//
LOCUS       DASSVX010000076         4272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_2719, whole genome shotgun sequence.
ACCESSION   DASSVX010000076 DASSVX010000000
VERSION     DASSVX010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2719"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VFO53_03595"
     CDS             complement(<1..759)
                     /locus_tag="VFO53_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8875390.1"
                     /translation="MTLFLVNIGFMIAAVATNSFARRWLGTWLPQGWTASWYADAWKE
                     FQLASILWVTVEVVFAVVALALLIGVPAAYTLARRDFRGKRALMLLFLLPLMVPPITY
                     GIPLATVLYQTHVGGTLSGVILANLIPAAPFVILVMTPFIEQIDPNLESAARVFGANT
                     GRRFVHVLVPLLGPGILAASLLVLVRTIALFELTFLTAGPDSQTLVVALYYAVFAAGV
                     RAPQSVDAMAMIYMAVTLVWLLIALRFVNPTQLVS"
     gene            complement(880..1725)
                     /locus_tag="VFO53_03600"
     CDS             complement(880..1725)
                     /locus_tag="VFO53_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8875391.1"
                     /translation="MGFDRALLLVVPAALFMLLLFIYPFVYGLVLSFEPKDGGWLANY
                     HKFFTTDNLWTTIGKTLQLALPATLINVFVALPIAYKMRVKSRWQRWMTTLLVVPITL
                     GTVLIAEGMLTYFGPRGWLAQFLQVTHLYDGSIRLTHNYWGVLISLVISGFPFAFLLI
                     LSYITGIDPVLARAASTLGADPKAQFRHIYLPLLGPGLAMCFCLAFVQAFSVFPSAVL
                     LGAPAGPTRVISIAAYEAAFEQYDYSLASAIAMIMGAVQLVIVAFVLSLRGWFYRGPV
                     TGGKG"
     gene            complement(1749..2834)
                     /locus_tag="VFO53_03605"
     CDS             complement(1749..2834)
                     /locus_tag="VFO53_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875392.1"
                     /translation="MKHDFNELRLDRVTRRFAGAGGHAFNALNDLTLTVRRGEFIALL
                     GPSGCGKSTALNCIAGLMPLSGGTIKLDDTRIDTLPPERRGFGMVFQNYALFPHMSVA
                     KNIGFGLRMRGMAQSDAAPRVAHALRLVQLTGQEHKLPGQLSGGQQQRVAIARAIVIE
                     PPLILMDEPLSNLDAKLRLETRSEIRRIHRELGRATIYVTHDQDEALSLADRIVVMKD
                     GVVQQIGTPEDVYAQPANIHVARFMGYRNVLELPVTGERDGHVTLEAPDMKLTGVRKL
                     PLDSRRAAVAIRPEEITIATEPAATNVIGGRVDNVEYGGRDSLVEVVTATGTRLHVRA
                     GGKVSVGANVFVHVPPERVLVYPSELQ"
     gene            complement(3003..4196)
                     /locus_tag="VFO53_03610"
     CDS             complement(3003..4196)
                     /locus_tag="VFO53_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8875393.1"
                     /translation="MKRSVHSQGRRRAARAITAVAVATGALFAFGLDSATAQTPVAIN
                     VVDVAGDLALTQDAIQNFASKNPKLISKVTFTKAPAPELPGKLKAMQGAGRSDIDLVL
                     TGTDFLAAGIEQGLLVKILPDHSAKFPNLMTNYQPAAAKMQELAGDYGIEVAFMPAGP
                     LLEYNPDKVKQVPTTPQELLAWCKANPNRLIYARPANSGPGRTFVMGLPYILGDKDPK
                     NPATWDKTWAYLKELNSCIEYYPSGTGAVMKELGEGSRDMTVTMTGWDLNPRILGIVP
                     KSYKVAPFQHMTWVNDAHYMVIPKGVSAEKMNVVLDLMAYMLKPEAQALTYDKGYFYP
                     GPAVKGVPLSMAPKESQDAIKEFGRPEYDKWLSEFPHTQSLEAKGQVEMFRIWDQQVG
                     AAKTK"
BASE COUNT          746 a         1415 c         1422 g          689 t
ORIGIN      
        1 cgagacgagc tgcgtcggat tgacgaagcg gagcgcgatc agcagccaca cgagcgtcac
       61 cgccatgtag atcatcgcca tcgcgtccac cgactgcggc gcgcgcacgc cggcggcgaa
      121 caccgcgtaa tacaacgcga cgacgagcgt ctgcgaatcg ggcccagcgg tcaggaacgt
      181 gagctcgaac aacgcgatcg tgcgcacgag gacgagcagc gacgcggcaa ggatgccggg
      241 gccgagcagc ggcacgagca cgtgcacgaa cctccgcccg gtattcgcgc cgaacacgcg
      301 cgcggccgat tcgagattgg gatcgatctg ctcgatgaaa ggcgtcatca ccagaatgac
      361 gaacggcgcg gcgggaatca ggttcgcgag gatgacgccc gacagcgtgc cgccgacgtg
      421 cgtctggtag agcaccgtcg cgagcggtat cccgtaggtg atcggcggca ccatcagcgg
      481 caacaggaac agcagcatca gcgcccgctt gccgcggaag tcgcggcgcg caagcgtgta
      541 cgcggcgggg accccgatca gcagcgccag cgcgacgacg gcgaacacga cctcgacggt
      601 gacccacagg atcgacgcga gctggaattc cttccacgcg tcggcgtacc acgacgcggt
      661 ccatccctgc ggcagccacg tgccgagcca gcggcgcgcg aacgagttcg tcgcgaccgc
      721 ggcgatcatg aagccgatgt tgacgaggaa gagcgtcatc acgccccaca acaacgcgcg
      781 ccagacgcgc tcgccgaggg ccgtggcgcg cgaatggcgc actcccggga tggcggcgag
      841 gccgcgcgtg ccgatcgtcg cgttgtccat gcgtcgccgc taccctttgc cgccggtgac
      901 cgggcccctg tagaaccagc cgcgcagcga cagcacgaac gcgacgatca cgagctgcac
      961 tgcgcccatg atcatcgcga tcgccgacgc aagcgagtag tcgtactgct cgaacgccgc
     1021 ctcgtacgcg gcgatcgaga tgacacgcgt cggccccgcc ggcgcaccga gcagcacggc
     1081 cgacggaaac accgagaacg cctgcacgaa cgcaaggcag aagcacatcg cgaggccggg
     1141 cccgagaagc ggcaggtaga tgtggcggaa ctgcgccttc gggtcggcgc cgagcgtcga
     1201 cgccgcgcgt gcgagcacgg ggtcgatgcc ggtgatgtac gacaggatca gcagaaacgc
     1261 gaacgggaag ccggaaatca cgagcgagat cagcacgccc cagtagttgt gggtgaggcg
     1321 gatcgagccg tcgtacagat gcgtgacctg caggaattgc gcgagccagc cccgcgggcc
     1381 gaaatacgtc agcatgcctt cggcgatcag cacggtgccg agggtgatgg gcaccacgag
     1441 cagcgtggtc atccatcgct gccagcgcga cttgacgcgc atcttgtacg cgatcggcag
     1501 cgcgacgaac acgttgatca gcgtcgccgg caacgcgagc tgcagcgtct tgccgatcgt
     1561 cgtccacagg ttgtccgtcg tgaagaactt gtggtaattc gcgagccagc cgccgtcctt
     1621 cggctcgaac gacagcacga gcccgtagac gaacgggtag atgaacagca gcagcatgaa
     1681 cagcgccgcc gggacgacga ggagcaacgc ccgatcgaag cccatggttc gtgcctgcgc
     1741 agtaaccatc actgcagctc gctcggatac acgagcacac gctcgggcgg cacgtgcacg
     1801 aagacgttcg cgccgacact caccttgccg ccggcacgca cgtgcagccg cgttccggtc
     1861 gcggtgacga cctcgacgag tgaatcgcgg ccgccgtatt ccacgttgtc gacgcggccg
     1921 ccgatgacgt tggtggcggc gggttccgtc gcgatcgtga tctcctcggg gcggatcgca
     1981 acggcggccc gcctggagtc gagtggcagc ttgcgcacgc cggtgagctt catgtcgggt
     2041 gcttcgagcg tgacgtgccc gtcgcgctcg ccggtgaccg gaagctcgag gacattgcga
     2101 tagcccatga accgcgcgac atggatgttg gccggctgcg cgtacacgtc ctcgggcgtg
     2161 ccgatctgct ggaccacgcc gtccttcatc acgacgatgc ggtcggcaag cgacagcgcc
     2221 tcgtcctggt cgtgcgtcac gtagatcgtc gcgcggccga gctcgcggtg gatgcgccgg
     2281 atttccgagc gcgtctcgag ccgcagcttg gcgtcgaggt tcgacagcgg ctcgtccatc
     2341 aggatcagtg gcggctcgat cacgatcgcg cgcgcgatcg cgacgcgctg ctgctggcca
     2401 cccgaaagct ggcccggaag cttgtgctcc tgcccggtca actggacaag ccgcagcgcg
     2461 tgcgcgacgc gcggcgcggc gtcggattgc gccatgccgc gcatgcgcag gccgaagccg
     2521 atgttcttcg cgacgctcat gtgcggaaag agcgcgtagt tctggaacac catgccgaag
     2581 ccgcgccgct cgggcggcaa cgtgtcgatg cgcgtgtcgt cgagcttgat cgtgccgccg
     2641 gaaagcggca tgagtccggc gatgcagttg agcgcggtcg acttgccgca ccccgacggc
     2701 ccgagcagcg cgatgaattc gccgcgccgc acggtgagcg tcaaatcgtt cagcgcgttg
     2761 aacgcgtgcc ccccggcgcc ggcgaagcgc cgggtgaccc ggtcgaggcg aagttcgttg
     2821 aaatcatgct tcatgcaaga gtccggacga cggcgcctcg cggcagcacc gactgtgcat
     2881 tcagatttta gaacgccgcg ttgagcgccg gcgacagccg acgttcgaac gcggcgagtt
     2941 ggcccgtcgc gacggatgtc acgccgggaa cgcgcgcggc atcccgacgc gacggggtaa
     3001 cgctacttgg ttttggcggc ccccacctgc tggtcccaga tccggaacat ctcgacctgg
     3061 cccttcgcct cgagcgactg cgtatgcggg aattcgctca gccacttgtc gtactcgggc
     3121 cgtccgaact ccttgatcgc gtcctggctt tccttcggcg ccatcgacag cggcacgccc
     3181 ttcaccgcgg ggccgggata gaaatagccc ttgtcgtacg tcagtgcctg cgcttctggc
     3241 ttcagcatgt acgccatcag gtcgagcacg acattcatct tctcggccga cacgcccttc
     3301 gggatcacca tgtagtgcgc gtcgttgacc cacgtcatgt gctggaacgg cgcgaccttg
     3361 tacgacttcg gcacgatgcc caggatgcgc ggattgagat cccagccggt catcgtcacc
     3421 gtcatgtcgc gcgagccctc gccgagctcc ttcatcaccg cgccggtgcc gctcgggtag
     3481 tactcgatgc agctgttgag ctccttcaga tacgcccacg tcttgtccca ggtcgcggga
     3541 ttcttcgggt ccttgtcgcc caggatgtac ggcagcccca tgacgaacgt gcgcccggga
     3601 ccggaattgg cgggacgcgc gtagatgagc cggttcgggt tcgccttgca ccacgcgaga
     3661 agctcctgcg gcgtggtcgg cacctgcttg accttgtcgg ggttgtattc gagcagcggc
     3721 cctgccggca tgaacgccac ctcgatgccg tagtcgccgg caagctcctg catcttcgcc
     3781 gccgccggct gatagttcgt catcaggttc gggaacttgg cggaatggtc gggaagaatc
     3841 ttgacgagca gaccctgctc gatgccggcg gcgaggaagt cggtgccggt cagcacgagg
     3901 tcgatgtcgc tgcgtcccgc gccctgcatc gccttgagct tgcccggcag ttcgggcgcc
     3961 ggcgccttgg tgaaggtcac tttcgagatg agcttcggat tcttgctcgc gaagttctgg
     4021 atggcatcct gcgtgagtgc caggtcgccc gcgacgtcga cgacgttgat cgccaccggc
     4081 gtttgcgccg ttgcgctgtc gagtccgaac gcaaacagcg cgccggtcgc aaccgcgacc
     4141 gccgtgattg cccgcgccgc ccggcgcctt ccctgcgaat gcacgctgcg tttcatttac
     4201 gtcctcctgt gaatgggctc gtgcccgtcg atttcgacgc cgccttgtta tcgacgcctc
     4261 gctgaaactc gc
//
LOCUS       DASSVX010000077         5262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_252470, whole genome shotgun sequence.
ACCESSION   DASSVX010000077 DASSVX010000000
VERSION     DASSVX010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252470"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFO53_03615"
     CDS             <1..477
                     /locus_tag="VFO53_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HET8875394.1"
                     /translation="ICEDPTDEDREWFPSFAGSDWRKTLDFAMRNFKDESFIGQYLSP
                     RLIREFHLFAIADHEDERDLVVDSIHDERGYKRVRKLLAQQHAQHIRVPDVQVMRYER
                     DGDRSLTLRHHCRRGRPLTDAAAQVVAHLRRLWGFPVRLETWDGEARVGDVVECAA"
     gene            650..1105
                     /gene="trxC"
                     /locus_tag="VFO53_03620"
     CDS             650..1105
                     /gene="trxC"
                     /locus_tag="VFO53_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HET8875395.1"
                     /translation="MTAAAAPAESLHVACPHCTTTNRVARDRLADGPACGSCKQPLFP
                     RHPLALTKADFGRLVNGDTPLVVDFWASWCGPCRMMAPAYEQAAARVAPGVRLAKLDT
                     EAEPEIASRYGIRSIPTLIAFRNGREIARQSGALPLPQLIQWITTHAQI"
     gene            1092..1499
                     /locus_tag="VFO53_03625"
     CDS             1092..1499
                     /locus_tag="VFO53_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET8875396.1"
                     /translation="MRRSDPGDWMWAEACDLLDQAERLHRRFFRLDTSRPARALWEPP
                     VDMYETEREIVVVVAMPGVPADRVEVITGSDALVVRGERPLPFAGRPLAVRHLEIPYG
                     AFERRIPLPAGRFDAAAPELTHGCLLLRLRRIG"
     gene            1508..3949
                     /gene="lon"
                     /locus_tag="VFO53_03630"
     CDS             1508..3949
                     /gene="lon"
                     /locus_tag="VFO53_03630"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET8875397.1"
                     /translation="MTEETSTNPAPLSAEGRTANGAPANHAPVRGEASTRPLPDDALI
                     ILPVRNVVMFPGAVFPITVGRDRSRAAAQEAVRLERPLGVLLQSKADVDEPGPDDMHW
                     VGTSASVLRYITTPDGLHHAIARGLRRFRVLQFLDGWPFTVARVQFIDDATEVDAEIE
                     GRARALRERATEILQLLPQVPEEMVAALGAVEGPSQLADFIAGLMDVSAEEKQSLLET
                     FDLKARLDKLLSLLAHRIEVLKVSREIGERTRESISDVNRKHLLREQMRQIQKELGEG
                     EEGNAEIADLDKAISDAHMPEEVEKAARKELKRLERMPEAAAEYSMVRSYLEWLIELP
                     WAIAAQPPIDIAEARRILDEDHFGLEKIKKRILEYLAVRKLNPEGRSPILCFVGPPGV
                     GKTSLGQSIAKATGRKFARVSLGGVHDEAEIRGHRRTYIGALPGNIIQSIRKADTRNG
                     VMMLDEVDKLGMGGFHGDPSSALLEVLDPEQNSTFRDNYLAVPFDLSHVMFICTANVL
                     DTIPGPLRDRMEIIQLPGYTAQEKLQIARRYLVPRQLKATGLDAAQCEISDEALLTII
                     HDYTREAGVRNLEREIGHVFRNVAVRIAEGSVQKSVVGPDDLQPILGARKFEAEVAMR
                     SSVPGVATGLAWTPVGGDILFVEAARTPGNGKLILTGQLGDVMKESAQAALSLVKARS
                     ASLGIDALALEKSDIHVHVPAGATPKDGPSAGVAMFVALTSLLTSRAVRSDVAMTGEI
                     SLRGLVLPIGGVKEKVLAALRAGIRTVMLPMRNQRDLDDIPADARAQLDFVWMEDVDQ
                     AIATALSPVKAQAAS"
     gene            4117..4986
                     /locus_tag="VFO53_03635"
     CDS             4117..4986
                     /locus_tag="VFO53_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET8875398.1"
                     /translation="MRRLPFVAPVGLAFVLAASASLATPFVFRTGNPDGLMATASQPA
                     SAGKTEIESADDFLTTSTTSITGGSFTGLLTGGAAISAIGDITVEIYRVFPLDSDTAR
                     TPNVTTRTNSPSDVAFDDRDSAAGELSFTCSTLSGSFTAANSVLNGINKAPNQTTGGE
                     GAVTGNEVQCSVTFTTPFVLPADHYFFVPQVAVSGDGNFFWLSAPRPIVAPGTPFGPD
                     LQSWIRNTGLDPDWSRIGTDIVGGSPAPTFNATFSLVGETVPEPAMLALLLAAALAGG
                     IATAKPARRGQAA"
BASE COUNT          866 a         1810 c         1737 g          849 t
ORIGIN      
        1 atctgcgaag acccgaccga cgaggaccgc gagtggttcc cgtcgttcgc cggcagcgac
       61 tggcgcaaga cgctcgactt cgcgatgcgc aatttcaagg acgagtcgtt catcggccag
      121 tacctgtcgc cgcggttgat ccgcgaattc cacctgttcg ccatcgccga ccacgaggac
      181 gagcgcgacc tcgtcgtcga cagcatccac gacgagcgcg gctacaagcg cgtgcgcaag
      241 ctgctcgcgc agcagcacgc gcagcacatc cgcgttcccg acgtgcaggt catgcgctac
      301 gagcgcgatg gcgaccgctc gctcacgctt cgccatcatt gccgtcgcgg ccggccgctg
      361 acggacgccg cggcgcaggt cgtcgcgcat ctgcggcggc tgtggggctt cccggtgcgc
      421 ctcgaaacct gggatggcga ggcgcgcgtg ggcgacgtcg tggagtgcgc cgcgtaagcc
      481 cactcgttca gggatttcgc aggcgaaccg tcgcgggacc ggacgcaggc cgcaggatcg
      541 accggcgttt ccccgcttga atcccgggcg gcggccctca ttgtttgccc atgcgtacac
      601 cccgtcatgg ccgatcgtcc cgggcgtcgt gcatgggcag ggtagcccaa tgactgcggc
      661 tgcggcaccg gccgaaagcc tgcacgtcgc ctgcccgcat tgcacgacga cgaaccgcgt
      721 cgcgcgcgat cgccttgccg atggcccggc ctgcggcagc tgcaagcagc cgcttttccc
      781 gcgacatccg ctggcgctga ccaaggccga tttcggacgc ctcgtcaacg gcgacacgcc
      841 gctcgtcgtc gacttctggg cgtcgtggtg cgggccctgc cggatgatgg ctccggccta
      901 cgagcaggct gcggcacgcg tcgcacccgg cgtgcgcctg gccaagctcg acaccgaagc
      961 cgagcccgag atcgcctcgc gttacggcat tcgcagcatc ccgacgctga tcgcgtttcg
     1021 caatggccgg gaaatcgcgc gccaatcggg cgcgctgccg ctcccgcaac tcatccaatg
     1081 gataacgacg catgcgcaga tctgatcccg gcgactggat gtgggccgaa gcctgcgacc
     1141 ttctcgatca ggccgagcgg ctgcatcggc gtttctttcg cctcgacacg tcgcggcccg
     1201 cgcgcgctct ctgggagccg ccggtcgaca tgtacgagac cgaacgcgaa atcgtcgtcg
     1261 tcgtcgcgat gcccggcgtg cctgccgacc gggtcgaggt gatcaccggc agcgatgcgc
     1321 tggtcgtccg tggcgagcgt ccgttgccgt ttgccggccg cccgctcgcc gtgcgccacc
     1381 tggaaattcc ttacggcgcg ttcgagcgcc gcattccgct tcccgccggg cgcttcgacg
     1441 ccgcagcgcc cgagctcacc cacggctgcc tgctgctgcg cctgcgcagg atcggctgac
     1501 cctgacgatg accgaagaaa ccagcacgaa ccccgctccg ttgtcggccg aaggccgcac
     1561 ggccaatggc gcgcccgcga atcacgcgcc ggtgcgtggc gaagcttcca cgcgaccgct
     1621 ccccgacgac gcattgatca tcctgccggt gcgcaatgtc gtgatgtttc ccggcgccgt
     1681 gtttcccatc accgtcggtc gcgatcgctc gcgcgccgcg gcgcaggagg cggtgcgcct
     1741 cgaacgtccg cttggcgtgc tcctgcaaag caaggccgac gtcgacgagc cgggccccga
     1801 cgacatgcat tgggtcggga ccagcgccag cgtattgcgc tacatcacca cgcccgacgg
     1861 acttcatcac gcgatcgcgc gcggtttgcg acgctttcgc gtgctgcagt tcctcgacgg
     1921 ctggccgttc acggtcgcgc gcgtgcaatt catcgacgac gcgaccgagg tcgacgccga
     1981 gatcgagggg cgggcacgcg cgctgcgcga gcgcgccacc gagatcctgc agcttctgcc
     2041 gcaggtgccc gaggagatgg tggcggcgct cggcgcggtc gagggtccgt cgcagctcgc
     2101 cgatttcatc gccggcctga tggatgtcag cgccgaggaa aagcagtcgc tgctcgagac
     2161 gttcgacttg aaggcgcggc tcgacaagct cctttcgctg ctcgcgcacc gcatcgaggt
     2221 actgaaggtg tcgcgcgaaa tcggcgagcg cacgcgcgag tcgatcagcg acgtcaatcg
     2281 caagcacctg ctgcgcgagc agatgcgcca gatccagaag gagctcggcg aaggcgagga
     2341 gggcaacgcg gaaatcgccg acctcgacaa ggcgatttcc gacgcgcaca tgccggaaga
     2401 ggtcgagaag gccgcgcgca aggaattgaa gcgcctcgag cggatgcccg aggccgctgc
     2461 cgagtattcg atggtgcgca gctacctcga gtggctgatc gagcttccct gggcaatcgc
     2521 cgcgcagccg ccgatcgaca tcgcggaagc gcgccggatc ctcgacgagg atcacttcgg
     2581 actcgagaag atcaagaagc gcatcctcga atacctcgcg gtgcgcaagc tcaatcccga
     2641 aggccgcagc ccgatccttt gcttcgtcgg cccgccggga gtcggcaaga catcgctcgg
     2701 ccagagcatc gcgaaggcca ccggacgcaa gttcgcccgc gtgagcctgg gcggcgtgca
     2761 cgacgaggcg gaaatccgcg gccatcggcg cacttacatc ggcgcgctgc ccgggaacat
     2821 catccagagc atccgcaagg cggacacgcg caacggcgtg atgatgctcg acgaagtgga
     2881 caagctcggc atgggcggct tccacggcga tccgtcgtcg gcgctgctcg aggtgctcga
     2941 tcccgagcag aactcgacgt tccgcgacaa ctacctcgcg gtgccgttcg acctgtcgca
     3001 cgtgatgttc atctgcacgg cgaacgtgct cgacacgatt cccgggccgc tgcgcgaccg
     3061 gatggagatc atccagcttc cgggctacac cgcgcaggag aagctgcaga tcgcgcggcg
     3121 ctatctcgtg ccgcgccagc tcaaggcgac gggtctcgac gctgcgcaat gcgagatcag
     3181 cgacgaggcg ctgctcacga tcatccacga ctacacgcgc gaggcgggcg tgcgcaatct
     3241 cgagcgtgag atcgggcacg tgttccgcaa cgtggcggtg cgcatcgccg aaggcagcgt
     3301 gcagaagagc gtcgtcggcc ccgacgatct gcagcccatt ctcggcgcgc gcaaattcga
     3361 ggccgaggtc gcgatgcggt cgagcgttcc cggcgtcgcg accgggctcg cctggacacc
     3421 cgttggcggc gacatcctgt tcgtcgaggc ggcgcgcacg cccggcaacg gcaagctcat
     3481 tctcaccggc cagctcggcg acgtgatgaa ggaaagcgcg caggcggcgc tgtcgctcgt
     3541 caaggcacgc tcggcatcgc tcggcatcga cgctttggcg ctcgagaagt cggacatcca
     3601 cgtccacgtg cccgccggcg ccacgccgaa ggacgggccg agtgcgggtg tcgcgatgtt
     3661 cgtcgcgctg acgtcgctgc tcacgtcgcg cgccgtgcgc agtgacgtcg cgatgaccgg
     3721 cgagatcagc ctgcgcggac tcgtgctgcc gatcggcggc gtgaaggaga aggtgctcgc
     3781 agcgctgcgc gccggcatcc ggacggtgat gctgccgatg cgcaaccagc gcgatctcga
     3841 cgacatcccc gccgacgcgc gcgcccagct cgacttcgtg tggatggaag acgtcgatca
     3901 agccatcgcg acggcgctgt ccccggtgaa ggcgcaagcc gcgtcgtagc ggggaccgag
     3961 gcacgcgaag cgcgcgtcgc gggcgtcgaa atatttgacg aagcacgggc acgacggcgt
     4021 gagcaaaagc gataacgcgc ggattctgcg gcgaagttcg cggctggaac ggatgctgcc
     4081 tgcattcgtc cgttcatcga tccgggggag atcaccatgc gccgattgcc attcgtagcg
     4141 ccggtcgggc tcgcgtttgt gctggcagca tcggcgtcgc tcgcaacgcc attcgtcttt
     4201 cgcaccggca atcctgatgg actgatggcc accgcctcgc agccggcgag cgcaggaaag
     4261 accgaaatcg aatcggccga cgattttctc accacgtcga cgacgagcat taccggcggc
     4321 agcttcaccg gccttttgac cggcggagcg gctatttcgg ccatcggcga tatcaccgtc
     4381 gagatctacc gcgtgtttcc actggactcc gatacggctc gcacgccgaa cgtcacgacg
     4441 cggacgaatt cgccgtcgga cgtcgcgttc gacgatcgtg acagcgcagc aggggagctt
     4501 tcgttcactt gcagcacact gtcggggagc tttacggccg cgaacagcgt gctgaacggc
     4561 atcaacaaag cgcccaatca gacaacgggc ggcgaaggcg ctgtcaccgg caacgaagtg
     4621 cagtgcagcg tgacgttcac gacgccgttc gtgctgccgg ccgaccatta cttcttcgtc
     4681 ccgcaggttg cggtgagcgg cgacggaaac ttcttctggc tttccgcgcc gaggccgatc
     4741 gtagcgcccg gtacgccatt cggtcctgat ctgcaaagct ggattcgcaa cacgggtctc
     4801 gaccccgact ggtcgcgcat cggaacggac attgtcggcg gttcaccggc gccgacgttc
     4861 aacgccacgt tttcgctcgt cggcgaaacc gttcccgagc ccgcgatgct cgcgttgctg
     4921 ctcgctgcgg cgctcgctgg cggcatcgcg acggcgaagc ccgcgcgacg ggggcaagcg
     4981 gcgtagatcg tccccggcag cggctcgtcg tcgggcgctg ccacggttcg ccgccggtcc
     5041 gcctttcctg gcggacgaca tcgcctagaa tggcggcggt cgaaccttgc ccgtcggagc
     5101 gcgccgccac caatctctcg gcggcgcagc ttcggtttca ttcgcatccc atcccgcggt
     5161 cctgtgacca atcgcaccac cggaggccct gtgatccacg ttgtcagact cgtctcgacc
     5221 ctcgtcgcgt gcgccgctct ttttgcgatg cccttcgccg cc
//
LOCUS       DASSVX010000078         7656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_552502, whole genome shotgun sequence.
ACCESSION   DASSVX010000078 DASSVX010000000
VERSION     DASSVX010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552502"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="VFO53_03640"
     CDS             <1..1348
                     /locus_tag="VFO53_03640"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HET8875399.1"
                     /translation="VASLNFNTVSTAAKINTSNKQIGVQVRGGGAHLAIDVVGYFGAP
                     SGNGGKFFQQGGNAFGTAASLGTTDNQPLALLANGQPAMTYLPNATSPNIVGGHASNS
                     VDSSLSGQTIAGGGFAGDNCFDSATNTSARSCLNTTTGNDASIGGGASNMALGDAASV
                     AGGFGNTAAGIAATVAGGGGNTASGNHATVGGGRGNNASGHRATVVGGEDNVADHDSA
                     TVLGGRLNQAAAAVSVAAGEAAIAMHTKSFVWDAWNSGSATSFRSNAFQIHGENGLDI
                     EYGTRRADGGGTSWVFIGNAFPGQAIATSTGAFLSTGGTWVPSSDRARKTSVLPIDPA
                     AVLEAVMQVPITSWQYKVEDPGIRHIGPMAQDFYRAFGFGYNDKSIATIDADGVALAA
                     IQGLRKLLLEKDAEIDGQRREIVALKERMVEVDAIREDLAKLRGALADMQQVPASP"
     gene            1462..3561
                     /locus_tag="VFO53_03645"
     CDS             1462..3561
                     /locus_tag="VFO53_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HET8875400.1"
                     /translation="MREPFIAVAARLCSYAVPVVSILMCALSQARPIDAADALLAIDQ
                     QRVSVVERIVATWGPALARSSVSISIDDLRARLMSLRADRLFAASLAGTEAGVREATG
                     LATAATLPAATAETKALGDTAIDVVYTPVTPCRLVETRGTFAAVYQGNGTASHTPVPF
                     APNEIRSYTIQGGNGVCLTQLPGGLNPSAVQLQVFGIPTTAASGDIEILPQGGTFGST
                     ATMVYVSTIQFNTVSTAAKINTLNHQISVQIRGGGAHLAIDVVGYFAAPNGNGGQFFR
                     LGGNAFGATAVLGTSDNQPLNVNVNGQRVTRYEPNANSPNVIAGHANNGGTLGSGGQA
                     IGGGGAAGNDCFDPQSGLNTRSCGNRTTGFFAAIGGGFANMATDSGTVAGGSNNAAGV
                     ASAVGGGSSNAASGRWAAIAGGIANTVVFGDAATVAGGESNTASGDHATVGGGNGNTA
                     SGGATVAGGQLNTASAGASTVSGGVSNMASGGGATVAGGTGNTASGQYSFAAGHRAKA
                     TTNGTFMWADSREFDFQPSVPNFFGARATGGVGFTVAIDGTTGGVTQFCNLLPGNPSW
                     QCTSDRNAKENFAPVDGADILARLTAMPITTWNFKGADPAQRALGPTAQDFYAAFGLG
                     NDDTTIATSNLASVALAAIQGLDRAMQEKDARIDALERSLRDIRRTHERELSELRQAL
                     HALSSQVGAVSRDRAPR"
     gene            complement(3582..6194)
                     /locus_tag="VFO53_03650"
     CDS             complement(3582..6194)
                     /locus_tag="VFO53_03650"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HET8875401.1"
                     /translation="MDTVTADAADTIAPAPAASIGQWRRSLAEGRAGLRAQFAARSDP
                     TRLLRGLARLTDSIVADAWRAARLPAAASLVAVGGYGRGELFPHSDVDVLILLPHAAD
                     GACAEAIERLLTSLWDAGIELSHAVRTVDECESEMTADATIATSLLEHRHLAGSRALQ
                     RDFARMFDARLGLRTFYDAKALEQQQRHLKYHDAAYNLEPNVKESPGGLRDLQTVIWI
                     ARASRLGRSWRELARNGLMTDAEARAVARQQRLIADLRIRLHYLAGRREDRLVFDLQS
                     ALAAELRFADTPAKRASEQLMQRYYRAAKLVRQVNTLLLQNLHARLHPAATAPLPIDR
                     TWSRVDELLDLSDDTLFDAEPSAMLDAFLMLQRHPELRGMTARTLRALWRSRHRIDPA
                     FRRDPANRSRFMQIFREPRGLTHELRRMNLYGILGQYLPAFGAIVGQMQHDLFHVYTV
                     DEHILMVIRNLRRFTEAQHAHEYPLCSRLIGDFAHKEVLYLAALFHDIAKGRGGDHST
                     LGARDAQRFCLAHGLAPEDAALVAWLVAEHLTMSLTAQKQDITDPGVVNAFAARVGDE
                     RRLTALYLLTVADIRGTSPKVWNAWKGKLLEDLFHAARAALNGGTSEHASRDSVALRQ
                     RESQRLLRLYAVPDDAERRLWRELDIAYFQRHTADEIAWHARHLHWRADRIAPVVKGR
                     LAHEGAGVQVLVYLPDQPALFARICGFFGRANLSILDAKIHTTRHGYALDTFAIHEPS
                     NPAPAYRETITFIEYELTRALTTPGPLQPPGEGRISRQLRHFPLTPEIRIFPDDKGTH
                     FILEVVAGDRPGLLAQVAYLLAHANVNVVTARINTLGDRAEDVFLVDGARLHDEQAML
                     RLETTLYEALAVKR"
     gene            complement(6218..7012)
                     /gene="map"
                     /locus_tag="VFO53_03655"
     CDS             complement(6218..7012)
                     /gene="map"
                     /locus_tag="VFO53_03655"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009852158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET8875402.1"
                     /translation="MSVQLKSEADIAGMRVAGRLASELLDHLTPMVTAGVTTKEIDRA
                     AHDYMVNVQQTIPATLNYAPPGHSPYPASLCTSVNHVVCHGIPGEKKLKDGDIVNIDV
                     TVIKDGYHGDTSRMFFIGKPSIQARRLVEITYEAMWRGIRAVRAGAHLGDIGSAIQKF
                     AEGHGFSVVREFCGHGIGNQFHEDPQVLHYGRPGTGLKLQPGMIFTVEPMINAGRAGI
                     RGLADGWTIVTADHSLSAQWEHTVLVTQDGYEILTQSAGTPPPASE"
     gene            complement(7088..>7656)
                     /locus_tag="VFO53_03660"
     CDS             complement(7088..>7656)
                     /locus_tag="VFO53_03660"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET8875403.1"
                     /translation="KVQTKFARFTDADFARAGVRVVPPAVARRGAYVAKNVVLMPSYV
                     NIGAYVDEGTMVDTWATVGSCAQIGRNVHLSGGVGIGGVLEPLQANPTIIEDNCFIGA
                     RSEVVEGVIVEENSVLGMGVFLGQSTKILDRASGTVSYGRVPAGSVVVAGSLPAADAS
                     HSLYCAVIVKKVDAKTRAKTGINELLRA"
BASE COUNT         1310 a         2466 c         2652 g         1228 t
ORIGIN      
        1 cgtcgcgtcg ctcaacttca acacggtgtc gacggcggcg aagatcaaca cgtcgaacaa
       61 acagatcggc gtgcaggtac gcggcggcgg cgcgcacctg gcgatcgacg tcgtcggcta
      121 tttcggggcg ccgagcggca acggcgggaa gttcttccag caaggcggca acgcgttcgg
      181 gacggcggca tcgctgggga cgaccgacaa ccagccattg gcgttgctcg cgaacggtca
      241 gcctgcgatg acttatctgc cgaatgcgac gagcccgaac atcgtcggcg gccatgcgag
      301 caacagcgtc gattcatcgt tatcgggcca gacgatcgcg ggcggtggct tcgccggcga
      361 caattgcttc gactcggcca cgaacacgag cgcgcggtcg tgcctcaata cgacaacggg
      421 caatgatgca tcgatcgggg gcggggcgag caacatggcg ctcggggatg cggcgagcgt
      481 cgccggcggc ttcggcaaca cggcggccgg catcgccgcg acggtcgccg gcggtggcgg
      541 caacacggca agcggcaacc atgcaacggt cgggggcggc cgcggcaata acgccagcgg
      601 ccaccgtgcc acggtcgtcg ggggcgagga caacgtcgcc gatcacgatt cggcgaccgt
      661 gctcggcggc cgcctcaacc aggcggctgc agcggtgagc gtggccgccg gtgaagccgc
      721 gattgcgatg cacacgaaga gctttgtgtg ggacgcctgg aactcgggat cggcgacgag
      781 cttccgttcg aatgcgttcc agattcacgg cgagaacggc ctcgacatcg agtacggcac
      841 tcgccgcgcc gatggcggcg gcacatcgtg ggtgttcatc ggcaacgcct ttcccggcca
      901 ggcgatcgcg acgtccacgg gtgcattcct cagcaccggc ggcacgtggg tgccgagctc
      961 ggatcgcgca cgaaagacga gcgtgctgcc gatcgatccg gcagccgtgc tcgaggcggt
     1021 catgcaggtg ccgatcacct cgtggcaata caaggtagaa gatccaggca ttcgtcacat
     1081 cgggccgatg gcgcaggact tctatcgcgc gttcggtttc ggatacaacg acaaatcgat
     1141 cgcgacgata gacgccgacg gtgtcgcgct cgcggcaatc cagggcctgc gcaagctgct
     1201 tctggagaag gatgcggaaa tcgacggcca gcgccgcgag atcgtcgccc tgaaggagcg
     1261 gatggtcgag gtcgacgcga tccgcgagga cctggcgaag ctcaggggcg cgctggcgga
     1321 catgcagcag gtgcccgcat cgccgtagcg ggcgagcgcg tatcgactat cacagtcgtg
     1381 tcgacgcgga tgccgcgctt cgtcatttgc gttggcggac gtcgcgtctc ggcctccgcg
     1441 ggcgatcgaa gaggaggaat catgcgcgaa ccgttcatcg ccgttgctgc caggctctgc
     1501 tcgtacgccg tccccgtcgt gtcgatcctc atgtgtgcct tgtcgcaggc gcggccgatc
     1561 gacgcagccg atgcgctgct cgcgatcgac cagcagcgcg tgagcgtcgt cgaacggatc
     1621 gtcgcgacat ggggacctgc actcgccagg tcgagcgtgt ccatttcgat cgacgacctg
     1681 cgcgcgcggt tgatgagcct tcgcgccgac cggctattcg cggcgagtct cgcaggcacc
     1741 gaagcgggcg tgcgcgaagc gaccggcctt gccactgcgg cgactttgcc tgctgcaact
     1801 gcggagacga aagcgctggg cgataccgcg atcgacgtcg tctacacacc ggtgacgcca
     1861 tgccgtttgg tcgaaacgcg cgggacgttc gccgccgtct atcagggcaa cggtaccgcc
     1921 tcgcacacgc cggtgccgtt cgcgccgaac gagatccgca gctacacgat ccagggcggc
     1981 aacggcgtat gcctgacgca actgccgggc ggcttgaacc cgagcgccgt gcagttgcag
     2041 gtgttcggca ttccgacgac ggctgcatcg ggcgacatcg agatcctgcc gcagggcggc
     2101 accttcggca gcactgcgac gatggtgtat gtcagcacga ttcagttcaa cacggtctcg
     2161 acggcagcga agatcaacac cttgaatcat cagatcagcg tgcagatacg cggcggaggc
     2221 gcgcacctgg cgatcgacgt cgtcggctac ttcgccgcgc caaacggcaa cggtggacag
     2281 ttctttcgcc tgggcggcaa tgcctttggc gcgaccgccg tactgggaac gtcggacaac
     2341 caaccactga acgtcaatgt caacggccag cgcgtgacgc gctacgaacc gaatgcgaac
     2401 agcccgaacg ttatcgccgg tcacgcgaac aatggtggga cccttggctc gggcgggcag
     2461 gccatcggtg gcggtggcgc agcggggaac gactgcttcg atccgcaaag cgggttgaac
     2521 acgcgttcgt gcggcaatag aacgaccgga ttctttgccg cgatcggggg agggttcgca
     2581 aacatggcaa cggactccgg caccgtggcg ggaggatcga acaacgctgc gggcgttgct
     2641 tccgcggtcg gcggcggctc cagcaatgcg gcgagcgggc gctgggcagc gattgcgggc
     2701 ggtattgcga acacggttgt cttcggcgac gcggcgaccg tggccggagg cgagtcgaac
     2761 accgcgagcg gcgaccatgc aacggttggc ggcggcaacg gcaacaccgc gagcggcggc
     2821 gcaaccgtgg cagggggaca gctcaatacg gcgagcgccg gcgcgtcgac ggtctccggc
     2881 ggcgtctcca acatggcgag cggcggaggc gcaaccgtcg ctggcgggac cggcaatacg
     2941 gccagcggac aatacagctt tgcggccgga catcgagcga aggcaacgac gaacggcacg
     3001 ttcatgtggg ccgattcacg cgaattcgac ttccagccgt cggtcccgaa cttcttcggc
     3061 gcgcgcgcga cgggcggcgt aggtttcacc gtcgcgatcg acggcacgac cggcggcgtg
     3121 acgcagttct gcaatctgct tcccggcaat ccgagctggc agtgcaccag cgaccgcaat
     3181 gccaaggaga actttgcccc ggtcgatggc gccgacatcc tcgcgcgcct tacggccatg
     3241 ccgatcacga cgtggaattt caagggcgcc gacccggcac agcgcgcgct cggtccgacc
     3301 gcacaggatt tctacgcggc tttcggcctc ggcaacgatg acaccacgat cgccacgagc
     3361 aatctcgcaa gcgtcgcgct cgcggcgatc caggggctcg atcgtgcgat gcaggagaag
     3421 gatgcgcgca tcgatgcgtt ggagcgcagc ttgcgcgaca tcaggcggac gcacgaacgc
     3481 gaactgagcg agttgcgaca agcgttgcat gcgctgtcgt cgcaggtggg tgccgtgagt
     3541 cgcgaccgcg ctcctcgata gcctttaccg cggccgacgc atcaacgctt cacggcaagc
     3601 gcctcgtaga gcgtcgtttc gaggcgcaac atcgcctgct cgtcatgcaa tctcgcgccg
     3661 tcgaccagga acacgtcctc ggcgcgatcg ccgagcgtgt tgattctcgc ggtgacgacg
     3721 ttgacgttcg cgtgcgcgag taaataggcg acctgcgcga gcagccccgg ccggtcgccg
     3781 gcgacgacct cgaggatgaa gtgcgtgccc ttgtcgtccg gaaagatccg gatctccggg
     3841 gtcagcggga agtgacgaag ctgccgtgaa atgcgacctt cgccgggagg ctgcagcgga
     3901 ccaggcgtcg tcaacgcgcg ggtgagctcg tactcgatga acgtgatcgt ctcgcgatac
     3961 gcgggcgccg gattcgacgg ctcgtggatc gcgaacgtat cgagcgcgta gccgtggcgt
     4021 gtcgtgtgga tcttcgcatc gaggatcgag agattggcgc gtccgaagaa gccacagatg
     4081 cgcgcgaaga gcgccggctg gtcgggcagg taaacgagca cctgcacccc cgcgccttcg
     4141 tgcgcgagcc gccccttgac gaccggcgcg atgcgatcgg cgcgccagtg cagatgacgt
     4201 gcgtgccatg cgatctcgtc ggcggtgtgc cgctggaaat acgcgatgtc gagctcgcgc
     4261 cagagccttc gctcggcatc atcgggcacc gcgtagaggc gcagcagccg ttgcgactca
     4321 cgctggcgca acgccacgct gtcgcggctt gcatgctcgc tggtgccgcc gttcagcgct
     4381 gcgcgcgcgg catggaagag gtcctcgagc agcttgccct tccacgcgtt ccacaccttc
     4441 gggctcgtgc cgcggatgtc cgcgacggtc aggaggtaga gcgcggtcag gcgccgctca
     4501 tcaccaaccc tcgcggcgaa cgcgttcacg acccccgggt ccgtgatgtc ctgcttctgc
     4561 gcggtgagtg acatcgtcag atgctcggcc acgagccacg cgacgagggc cgcatcctcc
     4621 ggcgcaaggc cgtgcgcaag acagaaacgc tgcgcatcgc gcgcgccgag cgtcgaatgg
     4681 tcgccgccgc gccccttggc gatgtcgtgg aagagtgctg cgaggtaaag cacctccttg
     4741 tgtgcgaaat cgccgatgag tcgcgagcac agcggatatt cgtgcgcatg ctgcgcctcg
     4801 gtgaagcggc gcaggttgcg gatcaccatc aggatgtgct cgtcgacggt gtacacgtgg
     4861 aagaggtcgt gctgcatctg gccgacgatg gcgccaaacg cgggcaggta ctggccgagg
     4921 atgccgtaga gattcatccg tcgcagctcg tgcgtgagcc cgcgcggctc gcggaagatc
     4981 tgcatgaacc ggctgcggtt cgcggggtcg cgacgaaacg cggggtcgat gcgatgccgg
     5041 cttcgccaca gcgcgcgcag cgttcgcgcc gtcatgccgc gaagctcggg gtgccgctgc
     5101 agcatcagga atgcatcgag catcgcagaa ggctccgcgt cgaacagcgt gtcgtccgaa
     5161 aggtcgagca gttcatcgac gcgcgaccac gtccgatcga tcggcagcgg cgccgtcgcc
     5221 gcgggatgga ggcgcgcgtg caggttctgc agcagcagcg tgttcacctg gcgcacgagc
     5281 ttggcggcgc ggtagtagcg ctgcatcaat tgctcgcttg cgcgctttgc cggcgtatcg
     5341 gcgaagcgga gctcggcggc aagtgccgac tgcaggtcga acacgagccg atcctcgcgg
     5401 cggccggcga ggtagtgaag ccggatgcgc agatcggcga tcagccgttg ctgccgcgcg
     5461 acggcgcggg cttccgcatc ggtcatcagt ccgttgcgcg caagctcgcg ccacgaccgt
     5521 ccgagccgcg acgcccgcgc aatccagatc accgtctgca ggtcgcgcaa gccgccagga
     5581 ctttccttga cgttcggctc caggttgtag gcggcgtcgt ggtacttgag atgccgctgc
     5641 tgctgctcga gcgccttcgc gtcgtagaac gtgcgcaggc cgagccgcgc gtcgaacatg
     5701 cgcgcgaaat cgcgctgcaa cgcgcgcgag cccgcgagat gccggtgctc gagcagactg
     5761 gtcgcgatcg tcgcatcggc ggtcatctcc gattcgcatt cgtcgaccgt gcgcacggcg
     5821 tgtgacagct cgattcccgc atcccacagc gaggtcagca ggcgctcgat cgcctctgca
     5881 caggcgccat ccgccgcgtg cggcagcagg atcagcacat cgacgtcgga atgcggaaag
     5941 agctcgccgc ggccgtaacc gccgacggcg acgagcgagg ccgccgcggg caggcgcgcg
     6001 gcgcgccagg cgtcggccac gatgctgtcg gtcaaccgcg cgagtccgcg cagaaggcgc
     6061 gtcggatcgg agcgcgcggc gaattgcgcg cgaagcccgg cgcggccctc cgcgagcgac
     6121 cggcgccact gtccaatgct cgcggccggc gccggcgcaa tcgtgtccgc tgcgtcggcc
     6181 gttacggtat ccatgcgtcg gccctttccg ggcgcggcta ctcggacgcg ggtgggggcg
     6241 tgcccgccga ttgcgtcagg atctcgtaac cgtcctgcgt gacgagaacg gtatgttccc
     6301 actgcgcgga cagcgaatga tcggcggtga cgatcgtcca gccgtcggca aggccgcgta
     6361 tgcccgcgcg tccggcgttg atcatcggct cgaccgtgaa gatcatgccc ggctgcagct
     6421 tgagcccggt gcccgggcgg ccgtaatgca gcacctgagg gtcctcgtgg aactggttgc
     6481 cgatgccatg tccgcagaat tcacgcacga ccgaaaaacc gtgcccctcg gcgaatttct
     6541 ggatcgccga accgatgtcg cccagatggg caccggcgcg gacggcgcgg atgccgcgcc
     6601 acatcgcctc gtacgtgatt tccacgagac gccgcgcctg gatcgacggc ttgccgatga
     6661 agaacatccg gctcgtatca ccgtgatagc cgtccttgat gaccgtcacg tcgatgttga
     6721 cgatgtcgcc gtccttgagc ttcttctccc cgggaatgcc gtggcacacg acatggttga
     6781 ccgacgtgca gagcgacgca gggtacggcg aatggccggg cggcgcatag ttgagcgtcg
     6841 cggggatcgt ctgctgcacg ttcaccatgt agtcgtgtgc ggcgcgatcg atctccttcg
     6901 tcgtcacgcc ggcggtgacc atcggcgtca ggtgatcgag cagctcggac gcgaggcgcc
     6961 cggcgacgcg cattccggcg atgtcggctt ccgatttgag ctgaacggac atgggggcta
     7021 gtggtgtgta acgggggtca ccagggcgtc gatctgttcg cgttcgcgtc catggtgtca
     7081 acaggcacta ggcccgcagc agttcgttga tgccggtctt cgcgcgcgtt ttcgcgtcga
     7141 ccttcttgac gatgacggca cagtagagcg aatggctcgc atcggcggcg ggcaggctgc
     7201 ccgcaacgac caccgacccg gccggcacgc ggccgtagct caccgtaccg ctcgcgcgat
     7261 cgagaatctt cgtgctttgg ccgaggaaca cgcccatgcc cagcaccgag ttctcctcga
     7321 cgatcacgcc ttcgaccact tccgagcgtg cgccgatgaa gcagttgtcc tcgatgatgg
     7381 tggggttcgc ctgcagcggt tcaagcacgc cgccgatgcc gacgccaccg gacaggtgca
     7441 cgttgcgccc gatctgtgcg cacgagccga ccgtggccca cgtgtcgacc atcgtgcctt
     7501 cgtcgacgta ggcgccgatg ttcacgtacg agggcatcag cacgacgttc ttcgcgacgt
     7561 aggcgccgcg gcgcgccacg gccggcggca cgacgcgcac gccggcgcgc gcgaagtcgg
     7621 cgtcggtgaa gcgcgcgaac ttggtctgca ccttgt
//
LOCUS       DASSVX010000079         6786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_19237, whole genome shotgun sequence.
ACCESSION   DASSVX010000079 DASSVX010000000
VERSION     DASSVX010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19237"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="VFO53_03665"
     CDS             complement(<1..1568)
                     /locus_tag="VFO53_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HET8875404.1"
                     /translation="MPNLNQTIDRSLSISRGVATVLERRLRDAFTDYAARVRQAQAAY
                     AASGAPSGAATPQQALRDWWEYNVDFAQRSILFFDTLRQRGNNWIAHEAAGKPPVLAY
                     KYEMLSDGRTFERRVNHALVRIVPPRGVDVDDKLRPYIIIDPRAGHGPGIGGFKEDSE
                     VGVALSAGHPVYFVIFFPEPEPGQTLADVTDAEAEFVRIVAERHPESAKPVLVGNCQG
                     GWAVMMLAAARPDIAGALVINGAPMSYWAGNDGDNPMRYAGGLLGGAWLSLFASDLGG
                     GKFDGAHLVESFENLNPANTLWEKYHHLFDAVDEEAERFLDFERWWGGFYLMNEEEIR
                     WIVNNLFIGNKLAQGEARLGPGRYFDLKAIKQPIIVFASMGDNITPPQQAFNWISDLY
                     SSTEEIKANGQTIVGLIHEDIGHLGIFVSGKVAKKEHTQIVSVLKYIQALPPGLYGMK
                     IHETTGADGNVEYDVTLHERKLEDLKRLQKYDRVDEKPFEAVAAFSELTERAYELLLR
                     PAVREMAPEWAAKLL"
     gene            complement(1734..2156)
                     /locus_tag="VFO53_03670"
     CDS             complement(1734..2156)
                     /locus_tag="VFO53_03670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET8875405.1"
                     /translation="MANTIHVDVVSAEEQIYSGEAEFVVLPGMMGELGIYPRHTPLFT
                     QIRPGSLRIKPANSAGEELVFVQGGYLEVQPHLVTVLADTAIRAKDLDEKAALEAKRA
                     AEEAMQNKTSKEEIAHAEAELSIALAQLEAIRKLRHRA"
     gene            complement(2156..3571)
                     /gene="atpD"
                     /locus_tag="VFO53_03675"
     CDS             complement(2156..3571)
                     /gene="atpD"
                     /locus_tag="VFO53_03675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET8875406.1"
                     /translation="MDQRVTNQGTIVQCIGAVVDVEFPREQMPNIYDALKMDDIGLTL
                     EVQQQLGDGIVRTIALGSSDGLRRGMQVVNTGAPIMVPVGPKTLGRIMDVLGRPIDER
                     GPVDAEKEMSIHRSAPAFDELSPSQELLETGIKVIDLTCPFAKGGKVGLFGGAGVGKT
                     VTMMELINNIATAHSGLSVFAGVGERTREGNDFYHEMIESGVVNKDDLGKSKVAMVYG
                     QMNEPPGNRLRVALSGLTMAEFFRDEGRDVLLFIDNIYRFTLAGTEVSALLGRMPSAV
                     GYQPTLAEEMGRLQERITSTKTGSITSIQAVYVPADDLTDPSPATTFGHLDATVVLSR
                     DIAALGIYPAVDPLDSTSRQVDPNVIGEEHYNCTRQTQQTLQRYKELRDIIAILGMDE
                     LSPEDKLVVSRARKIQRFLSQPFSVAEVFTGTPGKYVSLKDTIKGFSMIVNGECDHLP
                     EQAFYMVGPIEEAFEKAKTLQ"
     gene            complement(3582..4472)
                     /gene="atpG"
                     /locus_tag="VFO53_03680"
     CDS             complement(3582..4472)
                     /gene="atpG"
                     /locus_tag="VFO53_03680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET8875407.1"
                     /translation="MAGSKEIRNKIKSVQSTRKITKAMEMVAASKMKKAQERMRSGRP
                     YVERAFGIAMHIAKANTEYRHPFLHKRETVKRVGVIVITTDKGLCGGLNTNVLRLMMQ
                     RFREWRGNGIEVDFVAIGNKGLGFLNRMGGNIVSSVVQLGDRPRLERLVGAVKVLMDE
                     YLDGRVDEVHIFSTTFVNTMKQDARQIRIVPIPEEYRTAAGELRTSQLSDGAWDYIYE
                     PDARELLDGMMRRYMEAVIFQMVNESIASEQSARMVAMKAASDNAGKIISELQLIYNK
                     TRQAAITKELSEIVGGAAAV"
     gene            complement(4514..6055)
                     /gene="atpA"
                     /locus_tag="VFO53_03685"
     CDS             complement(4514..6055)
                     /gene="atpA"
                     /locus_tag="VFO53_03685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET8875408.1"
                     /translation="MQLNPSEISELIRSRIQNLDTGAEVRTQGTVVSVTDGICRIHGL
                     SNAMQGEMLEFPGNTYGLALNLERDSVGAVVLGAYEHITEGDTVKCTGRILEVPVGPE
                     LIGRVVNSLGQPIDGKGPINAKMTDVIEKVAPGVIWRKKVSQPVQTGLKSIDAMVPVG
                     RGQRELIIGDRQTGKTAVAVDTMINQKGKDLICVYVAIGQKASTIKNVIRKLEEHGAM
                     AYTIVVVATASESAAMQYIAPYSGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEEYVEHFTKGEVKGRTGSLTALPIIETQA
                     GDVTAFVPTNVISITDGQIFLETDLFNAGIRPAMNAGISVSRVGGDAQTKVIKKLGGG
                     VRLALAQYRELAAFAQFASDLDEATRKQLDRGRLVTEIMKQPQYQPLQVNEMALTLFA
                     VNRGYLDDVPVEKALAFEAALRQFMKSKYAPIMDRIEQTKDMNADDEKALAAAIDDFK
                     KTGTY"
     gene            complement(6068..6604)
                     /locus_tag="VFO53_03690"
     CDS             complement(6068..6604)
                     /locus_tag="VFO53_03690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET8875409.1"
                     /translation="MAEPATIARPYAEAAFEIARDAQALPAWSDMLRFAATIVRDPRV
                     AEALDNPRLDAAAKESLLLSIGGDRFAGEARNFVRVLVESERVALLPQIAAMFDALKN
                     DAEATANATIESAFELSDAQVAELRGALEKRFGKKIEATVTVNRELIGGARVTVGDAV
                     LDGSVQAKLAAMRAQLRA"
     gene            complement(6619..>6786)
                     /locus_tag="VFO53_03695"
     CDS             complement(6619..>6786)
                     /locus_tag="VFO53_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET8875410.1"
                     /translation="IAAAKAEAQQEYQSAREQLRNQVADLAVAGAAKILQREIDPRAH
                     AELLDSIRREL"
BASE COUNT         1017 a         2298 c         2189 g         1282 t
ORIGIN      
        1 aggagcttcg ccgcccactc gggggccatc tcgcgcaccg ccggacgcag caggagctcg
       61 taggcgcgct cggtcaactc ggagaacgcg gcgaccgcct cgaagggctt ctcgtcgacg
      121 cgatcgtact tctgcagccg cttcagatcc tcgagcttgc gctcgtgcag cgtcacgtcg
      181 tattcgacgt tgccgtcggc gccggtcgtc tcgtggatct tcatgccgta caggcccggc
      241 ggcagcgcct gaatgtactt cagcaccgaa acgatctgcg tgtgctcctt cttcgcgacc
      301 ttgcccgaca cgaagatgcc gaggtggccg atgtcctcgt ggatcaggcc gacgatcgtc
      361 tggccgttcg ccttgatctc ctcggtgctc gaatagaggt cgctgatcca gttgaacgcc
      421 tgctgcggcg gcgtgatgtt gtcgcccatc gacgcgaaca cgatgatcgg ctgcttgatc
      481 gccttcaggt cgaagtagcg gccgggcccg aggcgcgcct cgccctgcgc gagcttgttg
      541 ccgatgaaga ggttgttgac gatccagcgg atctcctcct cgttcatcag gtagaagcca
      601 ccccaccagc gctcgaagtc gaggaagcgc tccgcctcct cgtcgacggc gtcgaacagg
      661 tggtgatatt tctcccagag cgtattcgcc ggattgaggt tctcgaagct ctccacgaga
      721 tgcgcgccgt cgaacttgcc gccgccgaga tcgctcgcga acagcgacag ccacgcgccg
      781 ccgagcaggc caccggcata gcgcatcgga ttgtcgccgt cgttgccggc ccagtacgac
      841 atcggtgcgc cgttgatgac gagcgcaccg gcgatgtccg ggcgcgccgc ggccagcatc
      901 atcaccgccc agccgccctg gcaattgccg acgagtaccg gcttcgcgct ttcgggatgc
      961 cgctcggcga cgatgcggac gaattcggct tccgcgtcgg tcacgtcggc cagcgtctgc
     1021 ccgggctccg gctcggggaa gaagatcacg aaatacaccg ggtgaccggc cgaaagcgcg
     1081 acgccgacct ccgagtcttc cttgaagccg ccgatccccg gaccgtggcc ggcgcgcggg
     1141 tcgatgatga tgtacggccg cagcttgtcg tcgacgtcga cgccccgcgg cggaacgatg
     1201 cggacgagcg catggttgac gcgacgctcg aacgtgcggc catcgctcag catctcgtac
     1261 ttgtacgcga gcaccggcgg cttgccggcc gcctcgtgcg cgatccagtt gttgccgcgc
     1321 tggcgcagcg tgtcgaagaa caggatcgag cgctgggcga agtcgacgtt gtattcccac
     1381 cagtcgcgca acgcctgctg cggcgtcgcg gcacccgacg gcgcacccga cgcggcgtac
     1441 gccgcctgcg cctggcgcac gcgcgccgcg taatcggtga acgcgtcgcg caggcgccgt
     1501 tcgaggacgg ttgcgacgcc ccgggaaatg gaaagcgaac ggtcgatcgt ctggttcagg
     1561 ttgggcattg cggattcctt gagcggcgtg gggcacgccg agggcggtgc tgcagcgcag
     1621 catcgtacac tgcgtagatg cgggtcccgc aaccgtaatg caaaccagag cgcccggaaa
     1681 cggcgaaggg cagccgcgcg gctgcccttc gatgaccgcg aaccggccgg tcgctaggcg
     1741 cgatggcgca gcttgcgaat ggcctcgagt tgcgcgagcg cgatcgaaag ctcggcttcg
     1801 gcgtgcgcga tttcttcctt ggacgtcttg ttctgcatcg cctcctcggc ggcccgcttc
     1861 gcttcgagcg cggccttctc gtcgaggtcc ttcgcgcgaa tggcggtgtc ggcgaggacc
     1921 gtcaccagat gcggctgcac ctcgagatag ccgccctgca cgaacaccaa ttcctcgccc
     1981 gccgagttcg ccggcttgat gcgcaacgag cccggccgga tctgcgtgaa cagcggcgta
     2041 tgccgggggt agatgccgag ctcgcccatc atgcccggca gcacgacgaa ctccgcctcg
     2101 ccggagtaga tctgctcttc ggcgcttacg acgtcgacgt ggatggtgtt ggccattatt
     2161 gcagcgtctt cgctttctcg aaggcttcct cgatcggccc gaccatgtag aacgcctgct
     2221 cgggcaggtg atcgcattcg ccgttgacga tcatgctgaa gcccttgatc gtgtccttga
     2281 gcgatacgta cttgccgggc gtgccggtga acacttcggc gacgctgaac ggttgcgaca
     2341 ggaagcgctg gatcttgcgc gcgcgggaca cgacgagctt gtcctcgggc gacagctcgt
     2401 ccatgccgag gatcgcgatg atgtcgcgca gctccttgta gcgctgcagc gtctgctgcg
     2461 tctggcgcgt gcagttgtaa tgctcttcgc cgatgacgtt cgggtcgacc tggcgcgacg
     2521 tcgagtcgag cgggtcgacc gcggggtaga tgccgagcgc cgcgatgtcg cgcgacagca
     2581 cgaccgtcgc gtcgaggtgg ccgaacgtcg tggcgggcga cggatcggtc aggtcgtcgg
     2641 caggcacgta gaccgcctgg atcgacgtga tcgagcccgt cttcgtcgac gtgatgcgct
     2701 cctgcaaccg ccccatttcc tcggccagcg tcggctggta gcccaccgcc gacggcatcc
     2761 gtccgagcag cgccgacact tcggttcctg ccagcgtgaa gcggtagatg ttgtcgatga
     2821 acagcagcac gtcgcggccc tcgtcgcgga agaactcggc catcgtgagt cccgacagcg
     2881 cgacgcgcag gcggttgccc ggcggctcgt tcatctgccc gtacaccatc gcgaccttcg
     2941 acttgccgag gtcatccttg ttgacgacgc ccgattcgat catctcgtga tagaagtcgt
     3001 tgccctcgcg cgtgcgctcg ccgacgccgg caaacaccga caggcccgaa tgcgccgtcg
     3061 cgatgttgtt gatgagctcc atcatcgtga ccgtcttgcc gacgccggcg ccgccgaaca
     3121 ggcccacctt tccgcccttc gcgaacggac acgtgaggtc gatgaccttg atgccggtct
     3181 cgagcagctc ctgcgacggc gacagctcgt cgaacgccgg cgcactgcgg tggatcgaca
     3241 tctccttttc ggcgtccacg ggaccgcgct cgtcgatcgg acggccgagc acatccatga
     3301 tccggccgag cgtcttcggt cccaccggca ccatgatcgg cgcgccggtg ttgaccacct
     3361 gcatgccgcg ccgcagcccg tcggacgagc cgagcgcgat cgtgcgcacg atgccgtcgc
     3421 caagctgctg ctgcacctcg agcgtcaggc cgatgtcgtc catcttgagc gcgtcgtaga
     3481 tgttgggcat ctgctcgcgc gggaattcga cgtcgaccac cgcgccgatg cactgcacga
     3541 tcgttccctg gttcgttact cgctgatcca tttcggtatc cctatacggc agccgcgccg
     3601 ccgacgattt cagacaattc cttcgtgatg gcggcctgcc gcgtcttgtt gtagatgagc
     3661 tgcagctcgc tgatgatctt gccggcgttg tccgacgcgg ccttcatcgc gaccatccgc
     3721 gccgactgct cggaggcgat gctctcgttc accatctgga agatgaccgc ttccatgtag
     3781 cgacgcatca tgccgtcgag gagctcgcgc gcatcgggct cgtagatgta atcccaggcg
     3841 ccgtcggaca gctgcgacgt gcgcaattcg cccgccgccg tgcggtattc ctcgggaatg
     3901 ggcacgatgc gaatctgccg cgcatcctgc ttcatcgtgt tgacgaacgt cgtcgagaag
     3961 atgtgcacct cgtcgacgcg gccgtcgagg tactcgtcca tcagcacctt cacggcgccg
     4021 acgagccgct cgagccgcgg ccggtcgccg agctgcacga cgctcgacac gatgttgccg
     4081 cccatccggt tcaggaagcc gaggcccttg ttcccgatcg cgacgaagtc gacctcgatg
     4141 ccgttgccgc gccactcgcg gaaacgctgc atcatcagcc gcagcacgtt cgtattgagg
     4201 ccgccgcaca ggcccttgtc ggtcgtgatg acgatgacgc cgacgcgctt caccgtttcg
     4261 cgcttgtgca ggaacgggtg gcggtactcg gtgttcgcct tcgcgatgtg catggcgatg
     4321 ccgaacgccc gctcgacgta cgggcgcccg gagcgcatgc gctcctgcgc cttcttcatc
     4381 ttcgacgccg cgaccatctc catcgccttg gtgatcttgc gcgtgctctg cacgctcttg
     4441 atcttgttgc ggatttcctt ggatcccgcc atttcgcctt atcctgccgt tgccgccgaa
     4501 gctgtcccgg cggttaataa gtgccggtct tcttgaagtc gtcgatcgcc gcggcgagcg
     4561 ccttctcgtc gtcggcgttc atgtccttcg tctgttcgat cctgtccatg atcggcgcgt
     4621 acttgctctt catgaactgg cgcagcgccg cctcgaaggc gagcgccttc tcaaccggaa
     4681 cgtcgtcgag atagccgcgg ttcaccgcga agagcgtcag cgccatctcg ttgacctgca
     4741 gcggctggta ctgcggctgc ttcatgatct cggtcacgag gcggccgcgg tcgagctgct
     4801 tgcgcgtcgc ctcgtcgaga tcggacgcga actgcgcaaa cgccgccagc tcgcgatact
     4861 gcgcgagtgc gaggcgcacg ccgccgccga gcttcttgat gaccttcgtc tgcgcgtcgc
     4921 cgcccacccg cgacaccgag atgcccgcgt tcatcgcggg acggatgccg gcgttgaaga
     4981 gatcggtttc gaggaagatc tggccgtcgg tgatcgagat cacgttggtc ggcacgaacg
     5041 ccgtgacgtc gccggcctgc gtctcgatga tcggcagtgc cgtgagcgag ccggttctgc
     5101 ccttgacctc gcccttcgtg aagtgctcga cgtactcctc gttgacgcgc gccgcccgct
     5161 cgagcagccg cgagtgcagg tagaacacgt cgccgggata cgcttcgcgc cccggcggcc
     5221 ggcgcagcag cagcgacacc tgccggtacg cccatgcctg cttcgtgagg tcgtcgtaga
     5281 tgatgagcgc gtcctgcccg cggtcgcgga aatactcgcc catcgtgcag ccggaatacg
     5341 gcgcgatgta ttgcatcgcc gccgattcgg acgccgtggc cacgacgacg atcgtgtacg
     5401 ccatcgcgcc atgctcctcg agcttgcgga tgacgttctt gatcgtcgac gccttctgcc
     5461 cgatcgcgac gtacacgcag atcaggtcct tgcccttctg attgatcatc gtgtcgacgg
     5521 ccaccgccgt cttgccggtc tggcggtcgc cgatgatgag ctcgcgctgg ccgcggccga
     5581 ccggcaccat ggcgtcgatc gacttcaggc cggtttgcac cggctgcgac accttcttgc
     5641 gccagatcac gccgggcgca accttttcga tgacgtcggt catcttcgcg ttgatcgggc
     5701 ccttgccgtc gatcggctgg ccgagcgaat tgacgacgcg cccgatcagc tcgggaccga
     5761 ccggcacctc gaggatgcgc ccggtgcact tgaccgtgtc gccctccgtg atgtgctcgt
     5821 atgcaccgag cacgacggcg ccgaccgagt cgcgctcgag gttgagcgcg agcccgtacg
     5881 tgttgcccgg gaactcgagc atctcgcctt gcatcgcgtt cgacagtccg tggatgcggc
     5941 agatgccgtc ggtgaccgac acgaccgtgc cctgcgtgcg cacttcggcg ccggtgtcga
     6001 ggttctggat gcgcgagcgg atgagctcgc tgatttccga tggattgagc tgcatgcaaa
     6061 cctcctgtta agcgcgtagc tgcgcgcgca tcgccgcaag cttcgcctgc accgagccgt
     6121 cgagcacggc gtcgccgacc gtcacccgcg cgccgccgat gagctcgcgg ttcacggtga
     6181 cggtcgcttc gattttcttg ccgaagcgct tctcgagcgc accgcgcaat tcggcaacct
     6241 gcgcgtcgct gagctcgaac gcgctttcga tcgttgcgtt ggcggtggcc tcggcgtcgt
     6301 tcttcagcgc gtcgaacatc gccgcgatct gcggcagcag cgcgacgcgc tccgattcga
     6361 cgagcacgcg gacgaagttg cgcgcctcgc cggcgaagcg gtcgccgccg atcgacagca
     6421 gcagcgactc cttcgccgcc gcgtcgagcc gcgggttgtc gagcgcctcg gcgacacgcg
     6481 ggtcgcgcac gatcgtcgcc gcaaagcgca gcatgtcgga ccacgcaggc agcgcctgcg
     6541 cgtcgcgcgc gatctcgaac gccgcttcgg cgtagggccg tgcaatggtg gcgggttctg
     6601 ccatgtcgtc gcgccgccct agagctcgcg gcggatcgaa tcgagcagct cggcgtgcgc
     6661 gcgcggatcg atttcgcgtt gcaggatctt cgccgcgccg gcgaccgcga ggtcggcgac
     6721 ctggttgcgc aactgctcgc gcgcgctctg gtactcctgc tgcgcctcgg ccttcgcggc
     6781 cgcgat
//
LOCUS       DASSVX010000080         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1185919, whole genome shotgun sequence.
ACCESSION   DASSVX010000080 DASSVX010000000
VERSION     DASSVX010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185919"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1891
                     /locus_tag="VFO53_03700"
     CDS             164..1891
                     /locus_tag="VFO53_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET8875411.1"
                     /translation="MATVAKPTQPFAGERTGLALFAALPRALTILLTAAAVLAPLSLI
                     VYQSLLDAPFFDAVKHVGVAAYEFIFADPDFWMALRNSLLIAAGMTVIAVPLGAVLAF
                     VMERTDFPGKRFIEPMLLVPSFVSPMVLSFGYVVAAGPVGFYSVWATDVFGVAPWGLY
                     SLAAMSVIAGLTHVPNVYIYASSALKNLGSDVEEAARVAGASPFRVAVSVSLPLIMPA
                     LLFSAVLIFFLGLELFGLPLVLGDPEGHLVLSTYLYKLTNKLGTPSYHLMAAVAVCIV
                     ALTIPLVLLQRRLLQNVRKYATIRGKAGRIRELPLGAWKWVAVALVALWLFVAVIVPI
                     SGIALRAFVTNWGFGVRLVEAFTLDNFREVFNEPTDVRAIWNTVLLGTVGGALTVACY
                     TAIGFATHRRNDGWSRVVDYLVLVPRAVPGLLIGLAFLWVFLFFPPLRPLRETIFSMW
                     LAYTVVWLAYGMRLISSSLLQVSTELEEAARTIGAPRGRISREITLPLIRHGLLGSWL
                     LVFMIFEREYSTGVYLLAPGSEVIGAELVSLWQGGAIDVVAALSLINIVLVFIGLAFA
                     LRFGVRLRD"
     gene            1903..2958
                     /locus_tag="VFO53_03705"
     CDS             1903..2958
                     /locus_tag="VFO53_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875412.1"
                     /translation="MAELTVENLHLAYGDNPILNGVALTLNRGEVVSLLGPSGSGKTT
                     LLRAVAGLEVPHQGRIVIGDKVVFDGARGVDIPAEGRNLGLVFQSYALWPHKTVFDNV
                     AYGLRLRSVRRADMQDRVQVALDQLSLGKLADRFPHQLSGGQQQRVALARALVYNPPV
                     ILLDEPLSNLDAKLREEARAWLRELIVRMQLSSLCVTHDQAEALAMSDRILLLNGGRI
                     EQQGTPEEMYSAPQTLFTADFMGSNNRFAGRVTEIRNGSALLEGEGFRLWGTAKPGAA
                     SGGDGVGMIRLERIRIAEGPGDNRLAAELSTSMYLGDKWEHLFHVGEARVRAYGDQPL
                     AAGRHWLEMPRQSFWVF"
BASE COUNT          431 a         1028 c         1018 g          590 t
ORIGIN      
        1 acaccgtgaa gcccattccg gtcagcgccg agatcgtgag ctatctcgat cagaagaagc
       61 ggctcgagtt cctctcggaa tggaagaagg ccctcgcctc cggcagcggc aaataggcac
      121 gccggccggg cgcgcgtagc ctgcacgcga ctcgagcgaa acgatggcaa cggtcgcgaa
      181 gcccacgcag ccgttcgcgg gcgagcgcac gggcctcgca ctgttcgccg cgctgccgcg
      241 ggcgctgacg atcctgctca ctgcggcggc ggtgctcgca ccgttgtcgc tgatcgtcta
      301 ccagagcctg ctcgacgcgc cgttcttcga cgccgtcaag cacgtcggcg tcgccgcata
      361 cgaattcatc tttgccgatc ccgatttctg gatggcgctt cgcaactcgt tgctgatcgc
      421 ggccgggatg acggtgatcg cggtgccgct cggcgcggtg ctcgcgttcg tgatggagcg
      481 caccgatttc ccgggcaagc gtttcatcga gccgatgctg ctcgtgccga gcttcgtctc
      541 gccgatggtg ctctcgttcg gctacgtcgt ggcggcgggt cccgtggggt tctattccgt
      601 ctgggccacc gacgtcttcg gcgtcgcgcc gtggggtctc tattcgctcg ccgcgatgtc
      661 ggtgattgcc gggctgacgc acgttccgaa cgtctacatc tacgcgtcgt cggcgctcaa
      721 gaacctgggc tccgacgtcg aggaagcggc gcgtgtcgcg ggcgcgtcgc cgtttcgcgt
      781 tgcagtctcg gtaagcctgc cactcatcat gccggcgctg ctcttttccg cggtgctgat
      841 cttcttcctc ggcctcgagc tcttcggcct gccgctcgtc ctcggcgatc ccgaaggcca
      901 tctcgtgctg tcgacgtacc tgtacaagct cacgaacaag ctcgggacgc cgtcgtatca
      961 cctgatggcg gccgtggccg tctgcatcgt cgccctgacg attccgctcg tgctgctgca
     1021 gcgccgcctg ctgcagaacg tgcgcaagta cgcaacgatc cgcggcaagg caggccgcat
     1081 tcgcgagctt ccgcttggcg catggaaatg ggtggccgtg gcgctggtgg cgctgtggct
     1141 cttcgttgcc gtgatcgtgc cgatcagcgg catcgcactg cgcgcattcg tgaccaactg
     1201 gggcttcggc gtgcgcttgg tcgaggcgtt cacgctcgac aatttccgcg aggtcttcaa
     1261 cgagccgacc gacgtgcgcg cgatctggaa caccgtgctc ctcggcacgg tcggcggcgc
     1321 gctcaccgtc gcctgctaca cggcgatcgg cttcgcgacg catcggcgca acgatggctg
     1381 gtcgcgcgtg gtcgactacc tcgtgctcgt tccgcgcgcc gtgccgggcc ttctgatcgg
     1441 gctcgcgttc ctgtgggtgt tcctgttctt cccgccgctg cgaccgttgc gcgagacgat
     1501 cttcagcatg tggctcgcct acacggtcgt gtggctcgcc tacggcatgc ggctcatctc
     1561 gtcgtcgctc ttgcaggtga gcaccgagct cgaggaggcg gcgcgcacga tcggcgctcc
     1621 gcgcgggcgc atctcgcgcg agatcacgct gccgctcatt cgccatggcc tgctcggctc
     1681 gtggctgctc gtcttcatga tcttcgagcg cgagtactcg accggcgtct accttctcgc
     1741 gcccggcagc gaggtgatcg gcgccgagct cgtctcgctc tggcagggcg gcgcgatcga
     1801 cgtcgtcgcc gcgctgtcgc tcatcaacat cgtgctcgtc ttcatcggtc tcgcattcgc
     1861 cttgcgcttc ggcgtaagac tgagggatta ggagccatcc gcatggccga gctgaccgtc
     1921 gagaacctgc atctcgccta cggcgacaac ccgatcctga acggggtcgc gctgacgctg
     1981 aaccgcggcg aggtggtgtc gctgctcgga ccctccggca gcggcaagac cacgctgctt
     2041 cgcgccgtcg ccgggctcga ggtgccgcat cagggcagga ttgtcatcgg cgacaaggtc
     2101 gtgttcgatg gcgctcgggg cgtcgacatt cccgccgagg ggcgcaatct cggcctcgtc
     2161 tttcagtcct acgcgctgtg gccgcacaag acggtgttcg acaacgtcgc gtacgggctg
     2221 cggctgcgca gcgtccggcg cgccgatatg caggatcgcg tgcaggtggc actcgaccag
     2281 ctctcgctcg gcaagctcgc cgaccgcttc ccgcatcagc tttccggcgg ccagcaacag
     2341 cgcgtcgcgc tcgcgcgcgc actcgtctac aacccgccgg tcatcctgct cgacgagccg
     2401 ctgtcgaacc tcgacgcgaa gctgcgcgag gaggcgcgcg catggctgcg cgagctgatc
     2461 gtgcgcatgc aattgtcgtc gctgtgcgtg acgcacgacc aggccgaagc gctggcgatg
     2521 tcggatcgca tcctgcttct gaacggcggc cggatcgagc agcagggcac gcccgaggaa
     2581 atgtacagtg cgccgcagac gctgttcacc gccgacttca tgggcagcaa caaccgcttc
     2641 gccggccgcg tcaccgaaat ccgcaacggc agtgcgttgc tcgagggcga aggcttccgg
     2701 ctttggggaa ccgcgaaacc gggtgcggca tcgggcggcg acggggtcgg gatgatccgg
     2761 ctcgagcgca tccggatcgc cgagggaccg ggcgacaacc ggctcgccgc ggagctttcg
     2821 acgtcgatgt atctcggcga caaatgggag catctttttc acgtcggcga ggcgcgcgta
     2881 cgcgcttacg gcgatcagcc gctcgccgcc ggccggcact ggctggagat gccgcgccag
     2941 tcgttctggg tgttttgaat cggccgcgaa ataatcctcc gcgggtgctg tttgcgtttc
     3001 ggcgcttcca ccatgaggag gacgagatga ccgcgcgcaa caaggtaggg attgccgtcg
     3061 ccgcgtt
//
LOCUS       DASSVX010000081        23248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_502740, whole genome shotgun sequence.
ACCESSION   DASSVX010000081 DASSVX010000000
VERSION     DASSVX010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 23248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23248
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502740"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1344)
                     /locus_tag="VFO53_03710"
     CDS             complement(85..1344)
                     /locus_tag="VFO53_03710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HET8875413.1"
                     /translation="MNPLPTHQSGRADVGGRRPASSTARLLDTMLQGTSIPCRIVFAN
                     GVEYRNSESAPEFTIIFRTRRGELRLLRYGHVGLLESYFDADVDVEGNFALAFRVALD
                     RGFSQRPNALVRLRNRWHEARFSNRSIAQAKANARAHYGLGRAFYEPWLDRIGMMYTC
                     AYWKEGTRTLEDAQRNKMDHVCRKVQLKAGERFIDVGCGWGGLLFHAWRNYGALGTGI
                     NTATEQVVELREDIARQGLGDRIRVVECDFREMPGQYDKLLSIGTLEHAGPDCLRDVV
                     RAHADAMKPGALGVIHFIGHVGSFDTEFYIRKYIFPGGWIPSLHEAIDAMDANGLEVL
                     DVENLRRHYALTLDEWATRFDESWDAIHALDPARFDERHRRIWRTYLWSCAEMFRART
                     SRTHLFQVTVSKGNVTDYPMSRAFLYP"
     gene            1608..3188
                     /gene="gshA"
                     /locus_tag="VFO53_03715"
     CDS             1608..3188
                     /gene="gshA"
                     /locus_tag="VFO53_03715"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HET8875414.1"
                     /translation="MADSEDRLAALPHDALLRLLRGIERESLRVRPDGTLATSAHPQE
                     LGSALTHPHITTDFSESQLELITGVQPSVAACLRELTEIHQFVYRRIGDDMLWSGSMP
                     CRLPPDDDIPIGRYGASNIGRLKSVYRAGLSHRYGRRMQTISGVHYNFSLPEEAMSAL
                     QAGASDTRAPSVFRTERYFAAIRNFRRCSWLPLYLFGASPAVCTTFVDGRPHRLQRLS
                     GDTLYLPHATSLRMGPLGYQSDAQSALAVSFNSLERYAASLHHALTEPYPPYVAIGVQ
                     GDDGRYRQLATTLLQIENEFYGTIRPKRRIRRGERPLHALTARGVEYIEVRSLDVEPF
                     SPVGIDALTMRFLDVFLLQCLLADSPPDTPAEIARIAGNQFRVAERGREPGVRLMLAD
                     GEIALADWGLDVLRQCAPVAQALDEAAGGREHRDALDVASERLHDPTRTPSARVLREL
                     HERYASSYIEFALAHSLAHRAAMLALPSDASAAARLERMAGESLAEQQRLEETDDVPF
                     ELFRRQYLALDLMSGMPT"
     gene            3273..3668
                     /locus_tag="VFO53_03720"
     CDS             3273..3668
                     /locus_tag="VFO53_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HET8875415.1"
                     /translation="MALTDNELSELAQQLDARQRTLASDVHDELVEPERREYGMLAGA
                     LPGDSGDQSVADAQADLSFVMTDRYADELQAIELAKARMRDGTYGTCIDCGAEIGFER
                     LRAYPTAERCIQDQERVEKTYVQVNKPTL"
     gene            complement(3761..4237)
                     /locus_tag="VFO53_03725"
     CDS             complement(3761..4237)
                     /locus_tag="VFO53_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET8875416.1"
                     /translation="MTDTAETNSNATFAALCRGIVEAASEAIVFADRDGRIRLWNRGA
                     ELVFGYTAAEVVGQSLDVIIPERLRRAHWDAYDRSIETGRTKHTDRVLTTRSMHKSGN
                     KLYVDLSFGLVKDANGFVLGAFAIGRDCTARYLADGATKARLQDLETKLADASRST"
     gene            4398..5546
                     /locus_tag="VFO53_03730"
     CDS             4398..5546
                     /locus_tag="VFO53_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875417.1"
                     /translation="MNALASEFRDVLSGPHEPPCLSLYQPTHRRQPDRRQDSIRFGNL
                     VKALEDSLRRDYPARDVQPLLAPFHALGANRTFWNHGQDGLAVFAAADLFRVYQLPRS
                     VPERTIVADGFHIKPLLRIVQSADRFHILGLNRREACLFEGNRDGVTEIDLVPGLGRM
                     VTDMPIGDVDPALRATRVYESTLGAPTTRLGSDATQDVVDHDTDQFFRLVDQTVIARH
                     SRPAGLPLLLAALPEYHHRFRAISQNPLLANVAIDVFPDAISQDALRERAWRAMLPRY
                     LQRLDRLSDAFHVAKARNAATDDVRRAAEAAVAGRVATLLLDAERTIAGRLDGTTGAV
                     MLDDIDDADVDDVLDDLGEQVLKNGGDVVVVPAERMPSSTGVAATYRY"
     gene            5594..6415
                     /locus_tag="VFO53_03735"
     CDS             5594..6415
                     /locus_tag="VFO53_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET8875418.1"
                     /translation="MANTVGQAGCNDFHCGGERCPVPREKGARTPLQTRCATARCGTS
                     LAGVARCKRQTRIAIEVASRHVAGVAMNGKDVQMRLREIAVALAALSLAGTVVAAGND
                     TSTSTSGMTNGSSSSPASTTQAGSSANDQSSPPAGASSSDPTSAPQAGSSQMGTSSSA
                     SQGGSASSEQNDPALVRSAQQALKQKGFDAGAVDGRIGPTTESALRNFQQAQGLPQSG
                     NLDQQTLAALGVDQNQSSTSGQGAQSQSSMSGQGSQTEGATSSPGSATQTSGSYK"
     gene            complement(6517..6993)
                     /locus_tag="VFO53_03740"
     CDS             complement(6517..6993)
                     /locus_tag="VFO53_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8875419.1"
                     /translation="MPNIGAVLKQEIARLSRREIRGQVEATRKASAQYRRHIAALRRQ
                     IATLERQMALLQRRVGNGSAASGSAGSTSEGAPKLRFVAKGLKAQRARLGLSAVDYAR
                     LVGVSPQSIYNWEQGHTSPRPAQVAIIAQLRSLGKREAQARLEQMDAKQAKRARGK"
     gene            7136..8977
                     /locus_tag="VFO53_03745"
     CDS             7136..8977
                     /locus_tag="VFO53_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004493975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HET8875420.1"
                     /translation="MSKAGPHIVRTTLKYGLGTVAVLLVAAAIFLVNLVWFRPWSLDL
                     FYEKVFAQVLFDEPELLSSVGLVEQFGITGHNGKLGDESPAHQQHRFERARHALADLH
                     AYPLARQSTSQRLSTHILEWYLERETEGEKYQFHNYPVNQLFGVQNEFPSFMANTHRL
                     LDARDCEYYLQRLAGVPVKFDQLLESLRLREQKHIVPPRFVVERVLREMNAFVAQPPA
                     QNILAASFRTRTAKIAGLSDAQRAAFNARVETAVNDTVYPAYRKLIDYFNGLLPKTTT
                     EDGVWKLPDGDAFYAYVLRTNTTTALRPQEVHDLGLREVARIEAEMRALLDANGLRGR
                     TIPEAMQALGNDPRFLFANDANGRAQALAEYTRLIAQAVERSKSLFVTVPKAKIEVRR
                     VPEFKESTSAGAYYEAGAFDGTRPGVFYANLRDMRTLPKWSMPTFAYHEGVPGHHWQI
                     STAQELEGVPQFRKLIPFTAYIEGWALYAEWLAHDAGWYDGDPFGDIGRLQAEMFRAV
                     RLVVDTGIHAQRWSRERAIDYMREKTGMAQADVTAEVERYIVAPGQACAYKIGMLEIQ
                     ALRTRAQTELGPRFDQREFHDVVLKNGAMPLEILDEQVVAYIQARKG"
     gene            9121..9441
                     /locus_tag="VFO53_03750"
     CDS             9121..9441
                     /locus_tag="VFO53_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HET8875421.1"
                     /translation="MAKYLFEARYAAEGAKGVAREGGTARRAAIAKMTESLGGKLEAF
                     YYAFGDVDAYVIAELPDHICAAAIALTVNQAGAASVKTIVLIPPEDMDKAGKKAVDYR
                     PPGR"
     gene            complement(9581..10555)
                     /locus_tag="VFO53_03755"
     CDS             complement(9581..10555)
                     /locus_tag="VFO53_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020617672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8875422.1"
                     /translation="MARTIRWGIIGCGDVAEVKSGPGFQKAAHSTLVAVMRRDAARAE
                     DFARRHGVPRWHTDADAILDASDIDAVYIATLPQTHHDYALRAAAAGKAVYVEKPMAM
                     DAAECDAMIATCAAAGVPLWVAYYRRALPRFLAVRGLLREGVIGEVREVVSMQMQRAP
                     DPRAPGAWRLDTARAGGGAFFEGVVHTLDVLDFLFGPMEVVTSSATSRSEDPRLEDLV
                     DARYRFAAGVTGRGHWDFAAEADREFNEIVGMHGRLRFSTTRDVPIECWRGGACEVIP
                     IADPPHVQQPLIQTIVDEQNGMGRCPSTGQSAARTARVVDRMLSPLRR"
     gene            complement(10560..11327)
                     /locus_tag="VFO53_03760"
     CDS             complement(10560..11327)
                     /locus_tag="VFO53_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HET8875423.1"
                     /translation="MTAALVTGGARGIGRAIAVALARNGFDIGCVDREPAAAGQATVD
                     AVRGAGGACLYLEADIADTTAHAALVQKIESELGPIRCLVNNAGVTSLVRGDLLALTE
                     ASFDRTLAVNLRGTFFLTQCVARAMIASRAARPDAYRSIVTITSANAEIVGVNRGDYG
                     VSKAGLAMASKLFAARLAGEGIHVFEVRPGIIRTEMTGPATAEYDRLIADGGVPIARW
                     GEPADVAVTVATLACGGLAFATGEVIHVGGGLHLHRV"
     gene            complement(11372..12046)
                     /locus_tag="VFO53_03765"
     CDS             complement(11372..12046)
                     /locus_tag="VFO53_03765"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HET8875424.1"
                     /translation="MNERQARDEIGRVGQSLFARGYVHATAGNISVRLDDADGGGFLI
                     TPTDACLGFIDPARLARVDHGGRQLSGDPASKTLALHRRIYDAARAFDGDTRAIIHTH
                     STHAVALTLRCADAELLAPITPYFVMKVGHVPVVPYARPGAPALADAVAECIRRYGEA
                     GTPIRSVMLARLGPTAWHASAASAMAVIEELEETARLAMLVDPPPAPLGEAAIDELRR
                     AFGARW"
     gene            complement(12043..13371)
                     /gene="otnK"
                     /locus_tag="VFO53_03770"
     CDS             complement(12043..13371)
                     /gene="otnK"
                     /locus_tag="VFO53_03770"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HET8875425.1"
                     /translation="MAAAPVVKRARPRDAVRPSGLLLGAIADDFTGATDLANNLARHG
                     MRTMQTIGVPEAPPPEGIDAIVVALKSRTAPVDEAVALARKALKWLDAHGARQFYFKY
                     CSTFDSTPNGNIGPVIDALMDALAGRAGAALTVATPAFPDNGRTVYNGHLFVGHALLN
                     ESGMQDHPLTPMRDANLVRVLQAQSRRRVGLVEHRAVREGTDAIRARLAALEHDGIGV
                     AIVDAIENDDLCRLAAALAERPLVTAASGLAIGLPANFGLVASDRASALPPPRGRRAI
                     VAGSCSTATQRQVRAFVERGGRAFTIDPLRLSNDDMVREALAWSARQPADETLLVSST
                     AAADAVAAVQSRLGVERAGALVERALTAIARGLVEQGIGQLVVAGGETSGACVEALRI
                     ASLRIGPQIDPGVPWCHAESPASGGGLHLALKSGNFGGDDFFTRAFAVLG"
     gene            complement(13374..14195)
                     /locus_tag="VFO53_03775"
     CDS             complement(13374..14195)
                     /locus_tag="VFO53_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET8875426.1"
                     /translation="MAHVPAERCLAIIELLAEDASARPLGDIAEALGLPKSGAHRLLT
                     TLCELGWAEQDIDTSFYRLTMRLATLGQKFYAATGIPDVCQPLLDRLARESREFARLA
                     VVDGHSLVWVAHAQGATSGLVYQPSLATNTVPLSVTASGKAWLATFGVERAIECIEAN
                     GGFAQADRYGPKATRSLDAFLRELKATMRRGYGLAMSEAEPGVIAIAAAIRAGPQGSA
                     LGTVSVAGPSVRMTDERVAALAPLVVRCASELSQLWHLRRVARPPTPAGGVAVAA"
     gene            complement(14216..15019)
                     /locus_tag="VFO53_03780"
     CDS             complement(14216..15019)
                     /locus_tag="VFO53_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET8875427.1"
                     /translation="MKLGGATRLFAIIGDPVAQVRSPAVYTERFAREGIDAVMIPVHV
                     PAAQFDAIVPGLLRIANLDGVVVTVPFKGRMLPFADRLGATAACIGAVNALRREADGT
                     WTGDMFDGEGFVHGAQRKGLRLEARRVALFGAGGAGSAIACALARAGVASIALIDADE
                     ARAHMLAARFGAAFPSVAFACADAVPDGSDMIVNATPVGMADADGLPGVIAGLRPDAV
                     IGDVVIREAPTPIIRLAQRERVPYVTGLDMHAGQADALMSFLAPVHTPR"
     gene            complement(15115..16734)
                     /locus_tag="VFO53_03785"
     CDS             complement(15115..16734)
                     /locus_tag="VFO53_03785"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET8875428.1"
                     /translation="MEKISADDVAHLIQDGDAILISGSGGGHAVPEALLAAVERRFLA
                     EGVPRGLTSVSVVGVGDRAALGASHLAHEGLLARAITSALVDSPGLVRLAAEDKIEAY
                     TLPQGVLSQLMRDMAAGRPGLITRTGLDSFVDPRRQGGRQSPRTPPDFVEVVTLSGEE
                     WLLFKPVPVSVAFLRGTTADEDGNVTMEEEAVLGEMLAMAQATRRAGGIVVVQVKRIA
                     RRNTLAAKSVRIPGILVDFVVVEPEQRQTYLTYYDPSYSGELRIPTGDIKPLQFGPRK
                     VIVRRAAMELFPGAICNLGAGVSTGLSTIVAEEGLLDTVTLTNEQGIIGGAPITGRDS
                     GGGQNFAAMVEQPAQFDFYDGGGLDLAFLSFAEVDPAGNVNVSRFGDRIIGVGGFINI
                     SQNARTVVFGGTLTAGELDIEWEQGRTVIRREGRHRKFVPELEQVCYSAAIGRQRGQT
                     TLFVTERAVFRIGADGLELIEIAPGVDAERDVIAQMGFRPRIAAKLATMDERIFAPGL
                     MGLDALVNARPRQHRSARVAQWHAARAAASR"
     gene            complement(16799..17548)
                     /locus_tag="VFO53_03790"
     CDS             complement(16799..17548)
                     /locus_tag="VFO53_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875429.1"
                     /translation="MSRSRHSPAASTAPAIRAADDAACAPALLGEAAVLIWNDVATEG
                     RAQFYDWHDKEHIPERLAIPGFRRGRRYARPGHSPEWMTLYEARGIDVLTSSDYLTRL
                     NTPTSLTRATLPYFRRTSRAVCRVACTQGSSSGGHVLALRLDGDAAAEEGFVSHMATE
                     AFPRALGHTGIVACHLFASDRGGSFIDTAESRTRQFDVPAWVILVEATTAAAAEAARG
                     HVDPVAVRERAVRLRDDGAVYMLEICRLGHA"
     gene            complement(17545..18342)
                     /locus_tag="VFO53_03795"
     CDS             complement(17545..18342)
                     /locus_tag="VFO53_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET8875430.1"
                     /translation="MDDATTRHADHPLVGLTHEGARAEIALTAGPLNLVTRPLLRALN
                     AVLATLEAAHELRCVILHAENARAFCAGSDIREFVHLQANASEEKILFEDMVVRRLAR
                     LAVPTIAAIDASALGGGLELALACDLRVARRGVMLGLTESRLGGLAGNGSLRLARLVG
                     PGRAKELLFTGDTITADVALAWGLVNRVSDGSALEAARELAATIASRGPLSNRLAKEL
                     VDAAHDGPLDAALTRSTVAQQRIFDSDDLHEGVAAFFEKRDPAFEGR"
     gene            complement(18345..19196)
                     /locus_tag="VFO53_03800"
     CDS             complement(18345..19196)
                     /locus_tag="VFO53_03800"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875431.1"
                     /translation="MSVAADVRASESAVRSGVPIELCGVSKTFGADSDEPFRALGEIS
                     MSIEAGEFVSVVGPSGCGKSTLMLMVAGLLSRSQGDIAVGGKPVLRPLTEVGIAFQDH
                     LLLDFRTAIDNVMLQADIRRLPRKPIEARAKELFEQLRLTPAMKKYPRQLSGGMRQRV
                     SLVRALVHDPSVIMMDEPFGALDALTRLQVRMDLEALWLRRRPTVLFITHSVEEAVGL
                     SDRIFVMSPSPGEIVDEIRVALPRPRPVVLGDAPQFGAYVNRIYRRFEQMGVLHGAGI
                     GAAPATP"
     gene            complement(19193..19696)
                     /locus_tag="VFO53_03805"
     CDS             complement(19193..19696)
                     /locus_tag="VFO53_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HET8875432.1"
                     /translation="MSETIRTSLRYADLHVGLAFRSPGRTITDADLVAFAGLTGDYSE
                     LHTSDVYAKASEFGRRVAHGMLGLAYAHGLMWARTGELREVAIAFLGISDWRFVGPIF
                     VGDTIFVDYAIVELRDSRSRPTQAIATFDVAVKNQERAVVQRGRKALLVSKVPLAALS
                     ASAGGSG"
     gene            complement(19740..20741)
                     /locus_tag="VFO53_03810"
     CDS             complement(19740..20741)
                     /locus_tag="VFO53_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8875433.1"
                     /translation="MQKCVKRLLVGGLAVAGLVTMFDAGAATPVTFQLNWYAGGPNAG
                     FAAAVAEGYYKDAGLDVTLVQGNGSGNTAQLVASGRAQLAYADAVAVSQLIAKGAPMK
                     IVSTIYQSNPNEVSALKKTGIKSIADLKGKKVGVPSGSSQTTMLPLFLKANHLTETDV
                     NLINMPMTSMVPSLLQGQVDAILGSMDAYQIQLEAQGAELDNYRFADYGVPTVSTSIF
                     ANTQYMKDNPAVIRNFIAASLKGWSFALDHPDKAIKDLKQVFPEMNEKVATAELAAIT
                     PLFCSGGAKYIGKAEDALWVKTQDLLSEVKLLPAGQDPKSYYTDEFLPAASGMRACK"
     gene            complement(20812..21684)
                     /locus_tag="VFO53_03815"
     CDS             complement(20812..21684)
                     /locus_tag="VFO53_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875434.1"
                     /translation="MANHALHDVSPGIERQPDWQTALPPAPTAGAHSSHRRRLRNLAW
                     SLLPPLTFVAMVGVWAAAVAAFKIPAYLLPGPGSIFSRIISDAPMLWSHSVTTLTEIL
                     LGFGLTVVTAIPLGLLIALSRTAKQIVYPPVMLMQLVPKIAVAPLFLVWLGFGMESKV
                     LLTVLMTFFPLLMASISGFQILDERLLYLTQSMGANRWQTFRYLRFPAALPVIFSGIK
                     TSATIAATAAIVAEFVGANRGLGYVLLKGTGTMDIELVFAVLVVLTAIGIAINYAVEL
                     SEWAMTPWQRSKNT"
     gene            22187..>23248
                     /locus_tag="VFO53_03820"
     CDS             22187..>23248
                     /locus_tag="VFO53_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875435.1"
                     /translation="MSFVVAALGLVSTVAVAQNDASLRAGANALLAIDQHRATVIERI
                     VSQWGDSLAPAGTVLDMAELRTSLATLRADHLLAASLAGSADGVRDVLATALASVPKP
                     GDGRAQTKALGEIADDVVYTPVTPCRLVETRGTFAAVYQGGGAFSGGTIRTYTLQGGN
                     GVCLAQLPEGIAPTAVQLQVFGIPINGAASGDIEILPQGATFGTTATEVYVGNVAFNT
                     VSTTAKVNVANNQIAVQVRGGGANLAMDVVGYFKRPGNYGGVQTIGGLASDATVSGGF
                     NNTATGAISVVAGGQSNTASGHASAVGGGEFNTASGIDSTVGGGEGNVVSGSNSMVPG
                     GFNNTASGVNSFAAGSSAVA"
BASE COUNT         3881 a         7786 c         7860 g         3721 t
ORIGIN      
        1 cacgatcccc ccgcgcttgc ggctgcgccg ctgcgctgcc ccgcaacggg acggcattcg
       61 cgtctcgggg cggccctgcg gcgctcacgg atacaggaat gcgcggctca tcgggtaatc
      121 ggtgacgttg cccttgctga ccgtgacctg gaagagatgc gtgcgcgacg tgcgcgcgcg
      181 gaacatctcc gcgcacgacc acaggtaggt gcgccagatc ctgcggtgac gctcgtcgaa
      241 gcgcgcgggg tcgagcgcgt gaatggcgtc ccagctctcg tcgaaccgcg tcgcccattc
      301 gtcgagcgtc agcgcatagt ggcgccgcag gttctcgacg tcgaggactt cgagcccgtt
      361 cgcgtccatc gcgtcgatcg cctcgtgcag gctcgggatc cagccgccgg gaaagatgta
      421 tttgcgaatg tagaactcgg tatcgaacga gccgacgtgg ccgatgaaat ggatcacgcc
      481 gagggcgccc ggcttcatcg cgtccgcatg cgcgcgaacc acgtcgcgca gacagtcggg
      541 gcccgcatgc tcgagcgtgc cgatcgacag cagcttgtcg tactggcccg gcatctcgcg
      601 aaagtcgcat tcgacgacgc gtatccggtc gccgagcccc tgccgggcaa tgtcctcccg
      661 caattccacg acctgctcgg tcgccgtatt gatgccggtg ccgagagcgc cgtagttgcg
      721 ccaggcgtga aacagaagcc cgccccagcc gcagccgaca tcgatgaacc tctcgccggc
      781 tttcaactgc accttgcggc agacatggtc catcttgttg cgctgcgcat cctccagcgt
      841 acgcgtgcct tccttccagt acgcgcacgt gtacatcatg ccgatgcgat cgagccacgg
      901 ctcgtagaac gccctgccga ggccatagtg cgcccgcgca ttcgccttcg cctgtgcaat
      961 cgagcggttg gagaatcgcg cctcgtgcca gcggttgcgc agcctgacca gcgcgttcgg
     1021 ccgctggctg aatccgcggt cgagtgccac gcggaatgcc agcgcgaaat tgccctcgac
     1081 atccacgtcg gcgtcgaaat acgactcgag gaggccgaca tggccgtagc gaagcagccg
     1141 aagctcgccg cggcgcgtcc gaaagatgat cgtgaactct ggcgccgact cgctgttccg
     1201 gtattcgacg ccgttcgcaa agacgatgcg gcagggaatc gacgttccct gcagcatcgt
     1261 gtcgagcaat cgcgccgtgc tgctcgccgg gcggcgtccg ccgacatcgg cacgaccgga
     1321 ttggtgggtc ggcaacgggt tcatgttcct cgtgttgctg acagtggtcg aacggcggcg
     1381 ggcgagcggc ccgcacggcc gaatgataat tcaatgactg gggtgtccgg gggaagttca
     1441 tgcggccggc gggacgggga cggcgagcgc ttgaagcgtc gcggtcggcg ccggaggcgc
     1501 gaaggcataa tgctggcggc gtcaggcgtg caacggcggg gtttgatcgc gcgatcgtgc
     1561 ccgatcggcg cggcgctcgt gatcggaacg ttcggggagg acgcaagttg gcggattcgg
     1621 aagaccggtt ggcggcgctg ccgcacgatg cgctgctgcg cctgttgcgt ggcatcgaga
     1681 gggagagcct gcgcgtacgc ccggacggca cgctcgccac gagcgcgcac ccgcaggaac
     1741 tcggctcggc gctcacgcat ccgcatatca cgaccgactt cagtgaatcg cagctcgagc
     1801 tcatcaccgg cgtgcagccg agcgtcgccg cgtgcctgcg cgagctgacc gagattcatc
     1861 aattcgtcta ccggcgcatc ggcgacgaca tgctgtggtc gggcagcatg ccatgccgcc
     1921 tgcctcccga tgacgacatt ccgatcggcc gctatggcgc gtcgaacatc gggcggctga
     1981 agagcgtgta tcgcgccggc ctgtcgcacc gatacggccg gcggatgcag acgatctcgg
     2041 gcgtccatta caacttctcg ctgcccgaag aggcgatgtc ggcgttgcag gcgggggcgt
     2101 ccgacacacg ggcgccgagc gtatttcgaa ccgagcgcta tttcgcggcg atccgcaact
     2161 tccggcgctg ctcgtggttg ccgctgtacc tgttcggcgc gtcaccagcc gtttgcacga
     2221 cgttcgtcga cgggcgcccg catcgtctgc agagattgtc gggcgacacg ctctacctcc
     2281 cgcacgccac gtcgctgcgg atgggcccgc tcggttacca gagcgacgcg cagagcgcgc
     2341 tcgccgtcag cttcaacagc ctcgaacgat acgcggcgtc gctgcaccat gcgctgaccg
     2401 agccgtatcc gccctacgtg gcgatcggcg tgcaaggcga cgacggccgc taccggcagc
     2461 tcgcgacgac gctgctgcag atcgagaacg agttctacgg caccatacgt ccgaagcggc
     2521 gcattcgccg cggcgagcga ccgctgcacg cgcttaccgc gcgcggcgtc gagtacatcg
     2581 aggtgcgaag cctcgacgtc gagccgttct cgccggtggg catcgacgcg ctgacgatgc
     2641 gcttcctcga tgtcttcctg ttgcagtgcc tgttggccga cagcccaccc gacacgccgg
     2701 cggagatagc aaggatcgcc gggaaccagt tccgtgttgc agagcgtggt cgcgagcccg
     2761 gcgtccggct catgctggcc gacggtgaaa ttgcgcttgc cgactgggga ctcgatgtgc
     2821 tgcgccagtg cgcgccggtc gcgcaggcgc tcgacgaggc ggccggcggc cgcgagcacc
     2881 gggacgcgct cgacgtggcc agcgagcgcc tgcacgatcc cacgcgcacg ccgtcggcgc
     2941 gcgtgctgcg tgagctgcac gagcgctacg ccagctccta catcgaattt gcgctcgcgc
     3001 attcgcttgc gcatcgcgcc gcgatgctgg cactgccgtc cgacgccagt gcggccgcgc
     3061 ggctcgagcg gatggccggc gagtcgctcg cggagcagca aaggctcgag gagacggacg
     3121 acgtgccgtt cgagctcttc aggcggcaat acctggcgct cgacctgatg agcggaatgc
     3181 cgacctgacg catcccgaca cttccgggcc gattcgtgcc ggttcacgga accctgcggg
     3241 cgctcgacct gaccaagctc agggggatcg acatggcact gaccgacaac gaactatccg
     3301 agctcgcgca gcagctggac gcacggcagc gaacgctggc aagcgacgtt cacgatgagc
     3361 tcgtcgaacc cgagcgtcgc gagtacggca tgctggcagg cgcgctgccc ggcgacagcg
     3421 gcgaccaatc ggtcgcggac gcgcaggccg atctcagttt cgtgatgacc gaccggtacg
     3481 ccgacgaact gcaggccatc gagctcgcca aggcgcgcat gcgcgacggc acgtacggta
     3541 cgtgcatcga ctgcggcgcc gagatcggct tcgagcgcct tcgcgcgtat ccgacggccg
     3601 agcgctgcat ccaggatcag gagcgcgtgg aaaagaccta cgtgcaagtg aacaagccga
     3661 cgctttgatc cgtatcgcgg cgttacttct gccaatggaa gcgcccgccg aacacaacga
     3721 tgcagtcatg ctgcgcgaca tgctgctcgg tcggcaccga ttacgtcgag cgtgacgcat
     3781 cggcgagctt cgtctcgaga tcctggagcc gcgccttcgt cgcgccgtcg gcgagatagc
     3841 gtgcggtaca gtcgcggccg atggcgaatg caccgagaac aaaaccgttc gcatccttga
     3901 cgaggccaaa gctcaggtcc acatacagct tgttgccgct cttgtgcatc gagcgcgtcg
     3961 tgagcacccg gtcggtgtgt ttcgtgcggc cggtttcgat gctgcggtcg tatgcatccc
     4021 agtgagcgcg gcgcaggcgc tcgggaatga tcacgtcgag gctctggcca accacctccg
     4081 ccgccgtata gccgaacacc agttcggcgc cgcgattcca caggcgaatg cgaccgtcgc
     4141 ggtcggcaaa gacgatggct tcggaagcgg cttcgacgat gccgcggcaa agtgctgcga
     4201 aggtggcgtt ggagttggtt tcggcagtat cggtcatggg cataaagcgg ttgtggccac
     4261 gcgccgctcg ggccgcggcg cgcgaaatac cgtcgtgagt ctagccgatg ccggcgcgga
     4321 ggcttcgggc gaaatgccga atgatcgggc gatcgcgcag tcgaacacgg gtgatcgact
     4381 ggaccgagga gacgacgatg aacgcccttg caagcgaatt ccgcgacgtg ctgtccgggc
     4441 cgcacgagcc gccatgcctg tcgctctatc agccaacgca tcggcggcaa cccgataggc
     4501 gccaggactc gatccgcttc ggcaatctcg tcaaggcgct cgaagattcg ctccgccgcg
     4561 attatccggc gcgcgatgtg cagccgctgc ttgcaccgtt tcacgcactc ggcgcgaaca
     4621 ggacgttctg gaatcacgga caggacgggc tcgcggtatt cgccgccgcc gacctgtttc
     4681 gcgtctatca gctaccgcgc agcgtacccg agcgaacgat cgttgccgat ggcttccata
     4741 tcaagccgct tttgcgcatc gtgcagtcgg ccgaccggtt tcacatcctt ggcctcaacc
     4801 ggcgcgaagc ctgtctgttc gagggcaatc gcgatggcgt caccgagatc gacctcgtgc
     4861 cgggtcttgg gcgcatggtc accgacatgc cgatcggcga cgtcgatccc gcgctgcgtg
     4921 caacgcgcgt gtacgagtcg acgctcggtg cgccgacgac acgccttggt tccgacgcga
     4981 cgcaggacgt cgtcgaccac gacaccgacc agttctttcg cctggtcgac cagacggtga
     5041 tcgcccgtca ctcgcgcccg gccggccttc cgctcctgct cgctgcgctg ccggaatacc
     5101 atcatcggtt tcgcgccatc agtcagaatc cgctgctcgc gaatgtcgcc atcgacgttt
     5161 tcccggatgc catctcgcag gatgcgttgc gcgagcgtgc gtggcgcgca atgctgccac
     5221 gctacctgca gcggctcgac aggctttccg atgcgtttca tgtcgccaag gcgcgcaacg
     5281 cagccaccga cgacgtgcga cgcgccgccg aggcggcggt cgcgggtcgg gtcgcgacat
     5341 tgttgctcga cgccgagcgc accatcgccg gtcgtctcga cggcacgacg ggcgctgtca
     5401 tgctcgacga tatcgacgat gccgatgtcg acgacgttct cgacgacctt ggtgaacagg
     5461 ttctgaagaa cggcggcgac gtcgtcgtcg tgcccgccga acgcatgccg tcgtcgactg
     5521 gcgtcgcggc gacctatcgc tattgaacga cgcgtgactt cgtgtgcgaa cgcaatgtcc
     5581 gaaaatgcct gacgtggcga acacggttgg acaggctggt tgtaatgatt tccattgtgg
     5641 tggcgaacga tgcccggtcc ctcgcgaaaa gggcgcccgg acgccattgc agacgcgttg
     5701 cgcgacagca cgctgcggca cgtcgcttgc aggtgtagca cgctgcaagc gccaaacgcg
     5761 cattgcgatt gaggtcgcga gccggcatgt cgccggtgtc gcaatgaatg gaaaggatgt
     5821 tcaaatgaga cttcgcgaaa ttgcagttgc actcgccgcc ctttcgttgg ccggcacggt
     5881 tgtcgccgca ggcaacgaca cgagcacatc gacaagtggc atgacgaacg ggtcgtccag
     5941 tagcccggca tcgaccacgc aggcgggatc gtcggcgaac gaccagtcga gtccaccggc
     6001 cggcgcatca tcgagtgacc cgacgtcggc gccgcaagcc ggatcgtcgc aaatgggaac
     6061 gtcgtcgagc gcgtcacaag gcggcagcgc ttccagcgaa cagaacgatc cggcgctcgt
     6121 gcgttcagcc cagcaggcgc tgaagcaaaa gggcttcgac gctggcgccg tggacggccg
     6181 gatcggaccc accaccgaga gcgcattgcg caacttccag caggcgcaag gcttgccgca
     6241 atcgggcaat ctggaccagc aaacgctggc ggcgcttggt gtggatcaaa accagagttc
     6301 gacgtccggc cagggcgcgc aaagtcaaag ctcgatgtca gggcagggct cgcagaccga
     6361 gggcgcgaca tcgagcccgg gctcggccac gcaaacgagc ggctcctaca agtagccgca
     6421 attcacgcgt cgccgcagct cgcttcagcc gcggcgtcgc catcctgagc agaccgtttc
     6481 agcgcgcgcc gtgcgtgtca cgaccgcggc aggcgatcat ttgccacggg ctcgcttcgc
     6541 ctgtttggcg tccatctgct cgagacgcgc ttgcgcttcg cgctttccga ggctgcgcaa
     6601 ttgcgcgatg atcgcgacct gcgcgggacg cgggctcgta tgtccctgtt cccagttgta
     6661 gatcgactgc gggcttacgc cgacgagacg cgcgtagtcc accgcagaaa ggcccaatcg
     6721 cgcacgctgc gccttgagac ccttggcgac aaagcgcagc ttcggcgccc cttccgacgt
     6781 gcttccggct gacccgctcg ccgcagaccc gttgccgaca cgacgctgca gaagcgccat
     6841 ctgacgctcc agcgtggcga tctgacggcg cagcgctgcg atgtgccgcc ggtattgggc
     6901 ggacgccttc ctggttgcct cgacctggcc gcgaatctcg cggcgcgaga gccgggcgat
     6961 ttcctgctta agtactgcgc cgatgttggg catggcactc cttgggttcg tggtgcacga
     7021 aggatagccg aagcgccggt aggggggcag cccgcccgca agggcttgcc ggccgccgct
     7081 gccggctttg ttgcataatc gcccgccccg ccggaatcgc gaaggaatcg aggccatgag
     7141 caaggcaggc ccgcacatcg tacggacgac gctgaagtac ggcctcggca cggtcgcggt
     7201 cttgctcgtc gcggcggcga ttttcctcgt caacctcgtc tggttccgcc cgtggagcct
     7261 cgatcttttc tacgagaagg ttttcgcgca ggtgctgttc gacgagcccg agctcctttc
     7321 gtcggtgggg ctcgtcgagc agttcggcat caccggccac aacggcaagc tcggcgacga
     7381 atcacccgcg catcagcagc accgcttcga gcgcgcgagg catgccctgg ccgatcttca
     7441 cgcctatccg ctcgcacgcc aaagcacttc gcagcggctt tcgacgcaca tcctcgagtg
     7501 gtatctcgag cgcgagaccg agggcgagaa gtaccagttc cataattatc cggtcaacca
     7561 gcttttcggc gtgcagaacg aatttccgtc gttcatggcg aatacgcatc ggctcctgga
     7621 tgcgcgggat tgcgagtatt acctgcagcg actcgccggc gtgccggtga agttcgacca
     7681 gctgctcgag agcctgcggc tgcgcgagca aaagcacatc gtgccgccgc gcttcgtcgt
     7741 ggagagggtg ctcagggaga tgaacgcgtt cgttgcgcag ccaccagcgc aaaacattct
     7801 cgcggcttcg tttcgaacgc gcaccgccaa aatcgcaggg ctgtccgacg cgcaacgcgc
     7861 tgcattcaac gcacgcgtgg agaccgcggt aaacgacacc gtgtatccgg cataccggaa
     7921 gctgatcgat tatttcaacg gcctgctgcc gaagacgaca accgaggatg gcgtctggaa
     7981 attgccggac ggtgacgcgt tttatgccta cgtgctgcga acgaacacca cgacggcgct
     8041 gcggccgcag gaggtgcacg acctcggatt gcgcgaggtg gcgagaatcg aagcggagat
     8101 gcgcgcgctg ctcgatgcga acggattgcg cggtcgcacg attcccgagg cgatgcaggc
     8161 gctcggcaac gatccgcgtt ttctctttgc gaacgacgcc aatgggcggg cgcaggcgct
     8221 cgccgaatac acgcgcctca ttgcgcaggc cgttgaacgg tcgaaatccc tcttcgtgac
     8281 cgtcccgaag gcgaagatcg aagtgcgtcg cgtgccggaa ttcaaggagt cgacatccgc
     8341 gggcgcctat tacgaagccg gcgcgttcga cggaacgcga ccgggcgtct tctacgcgaa
     8401 tctgcgcgac atgcgcaccc tgccgaagtg gagcatgccg acgttcgcgt accacgaggg
     8461 tgttcccggc catcactggc agatctcgac ggcacaggaa ttggaaggtg tgccgcagtt
     8521 tcgcaagctc attcccttca ccgcgtatat cgaggggtgg gcgctctatg ccgagtggct
     8581 ggcgcacgac gcgggctggt acgacggcga tccgttcggc gacatcggcc ggttgcaggc
     8641 cgagatgttc cgcgcagtgc gcctcgtggt ggacaccgga atccatgcgc agcgctggtc
     8701 gcgagaacgc gcgatcgact atatgcgcga gaagacgggc atggcgcagg cggatgtgac
     8761 ggccgaggtc gaacgctaca tcgtcgcccc gggtcaagcg tgcgcgtaca agatcggcat
     8821 gctcgagatt caggcgctac gcacgcgcgc acaaacggag ctcgggccga ggttcgacca
     8881 gcgcgagttt cacgacgtcg tgctgaagaa cggcgcgatg ccgctcgaaa tcctcgacga
     8941 gcaggtcgtg gcgtatatcc aggccaggaa gggataggcg ccgcgcgtat cgtcgcgcat
     9001 cgatcgactt cgttcggcca gcgaaagggt gcatgatgtt cgcgcgcgtt cgaggtcgac
     9061 ggcgcctttt gcgggtccgc gcggccctgg caatcgcgct caccagagga gaacgggaac
     9121 atggccaaat acctgtttga agcacgttac gccgcggagg gtgccaaggg cgtcgcccgc
     9181 gaagggggga cggcacgccg tgccgccatc gcgaaaatga cggagagcct gggcggcaag
     9241 ctggaagcgt tctattacgc attcggcgac gtcgatgcgt acgtcatcgc cgaattaccc
     9301 gaccacattt gcgccgccgc catcgcgctc accgtgaatc aagcgggtgc cgcgagcgtg
     9361 aaaaccatcg tcctgattcc gccggaggac atggacaagg cgggcaagaa ggccgtggac
     9421 tatcggccgc cgggtcgttg accggctgga atcgccgtcc ggttcgagtg ggcgccgttc
     9481 atcactcgcg gcgcccgctt ttgcgagaac cggaccacgc agtgcgcgag cggcgaacgt
     9541 gcgcgtagtt gcgcggggca agcccgcgag cgttcagcat tcagcgccgc aatggcgaga
     9601 gcatccggtc gaccacgcgc gcggtgcgcg ccgccgattg gccggtgctc gggcagcgtc
     9661 ccatcccgtt ctgctcgtcg acgatcgtct ggatcagcgg ctgctgcacg tgcggcgggt
     9721 cggcgatagg gatgacctcg cacgcgccgc cgcgccagca ttcgatcggc acgtcgcgcg
     9781 tcgtcgagaa ccgcaagcgt ccgtgcattc cgacgatctc attgaattcc cggtcggctt
     9841 cggccgcgaa gtcccagtgc ccccggccgg tgacgccggc ggcgaatcgg tagcgcgcat
     9901 cgacgagatc ctcgaggcgg ggatcttcgc tacgggacgt tgcgctcgac gtgacgacct
     9961 ccatcgggcc gaacagaaaa tcgaggacgt cgagcgtgtg cacgacgccc tcgaagaatg
    10021 cgccgccgcc ggcgcgcgcg gtatcgaggc gccacgcgcc gggcgcgcgc ggatcgggcg
    10081 cgcgctgcat ttgcatcgac acgacctcgc gcacctcgcc gatgacacct tcgcgcaaga
    10141 ggccgcgtac cgcgaggaag cgcggcaggg cgcgccggta ataggcgacc cacagcggca
    10201 cgcccgccgc ggcacaggtg gcgatcatcg cgtcgcattc ggcggcgtcc atcgccatcg
    10261 gcttctcgac gtacacggcc ttgccggccg ccgccgcacg caatgcgtag tcgtgatgcg
    10321 tctgcggcag cgtcgcgatg tagaccgcat cgatgtccga agcgtcgagg atcgcgtcgg
    10381 cgtcggtatg ccagcgcggc acgccgtggc ggcgcgcgaa gtcctcggcg cgcgccgcgt
    10441 cgcgccgcat caccgcgacc agcgtcgagt gcgccgcctt ctggaacccg ggaccgctct
    10501 tcacctcggc gacgtcgccg cagccgatga tgccccaacg gatcgtgcgt gccacgcggc
    10561 tagacacggt gaaggtgcaa tccgccgccg acgtggatca cctcgccggt cgcgaacgcg
    10621 aggccgccgc acgcgagcgt cgctacggtc acggccacat ccgcgggctc tccccagcgc
    10681 gcgatcggca cgccaccgtc ggcgatcagc cgatcgtact cggccgtcgc gggtccggtc
    10741 atctccgtgc ggatgatccc ggggcgcacc tcgaacacat ggatgccttc gccggcaagc
    10801 cgcgcggcga acagtttcga ggccatggcg aggccggcct tgctgacccc gtagtcgccg
    10861 cggttgacgc ccacgatctc ggcattggcc gaggtgatcg tgacgatcga acgataagcg
    10921 tccggccgcg ctgcgcgcga cgcaatcatc gcgcgcgcca cgcattgggt gaggaagaac
    10981 gtgccgcgca ggttgaccgc gagcgtgcgg tcgaagctcg cctcggtgag cgcgaggagg
    11041 tctccgcgca cgagcgaggt gacgcccgcg ttgttgacga ggcagcggat cgggccgagc
    11101 tccgattcga tcttctgcac cagcgccgcg tgcgcagtcg tgtccgcaat gtcggcctcg
    11161 aggtacaggc aggcgccccc ggcaccgcga acggcgtcga ccgtggcctg cccggccgcc
    11221 gcgggttcgc gatcgacgca gccgatgtcg aatccattgc gcgcgagcgc caccgcgatc
    11281 gcacggccga tgccccgcgc gccgccggtg acgagtgcgg ccgtcatgct tgcgttccgc
    11341 ggcccgctga gcgtcgcacg atcacggagg cttaccagcg cgcgccgaac gcgcgccgca
    11401 gctcgtcgat cgcggcctcg ccgagcggcg cgggcggcgg atcgacgagc attgcgagac
    11461 gtgcggtttc ctcgagctct tcgatcacgg ccatcgcgct ggccgccgac gcgtgccacg
    11521 cggtcgggcc gagtcgcgcc agcatcaccg agcggatcgg cgtgcccgct tcgccatacc
    11581 gcctgatgca ttcggccacg gcgtccgcca gcgccggtgc gccggggcgc gcgtatggca
    11641 ccaccggcac gtggccgacc ttcatcacga agtagggtgt gatcggcgcg aggagctccg
    11701 cgtccgcgca ccgcagcgtc agcgccaccg catgcgtgct gtgtgtgtgg atgatcgccc
    11761 gcgtgtcgcc gtcgaacgcg cgcgcggcgt cgtagattcg ccggtgcagc gcaagcgtct
    11821 tgctcgccgg gtcgccgctc aactgccgcc cgccgtgatc gacgcgcgcg aggcgcgccg
    11881 ggtcgatgaa gccgaggcag gcgtcggtgg gcgtgatgag gaagcccccg ccgtcggcgt
    11941 cgtcgagacg cacgctgatg ttgcccgctg tcgcgtgcac gtagccgcgc gcgaagaggc
    12001 tttggcccac tcggcctatc tcgtcgcgtg cctgccgctc gttcatccca gcaccgcaaa
    12061 tgcgcgcgtg aagaaatcgt cgccgccgaa gttgcccgac ttgagcgcaa ggtgaaggcc
    12121 gccgccggac gcgggcgact cggcatggca ccacggcaca ccgggatcga tctgcggtcc
    12181 gatgcgtagc gacgcgatgc gtagtgcctc cacgcaggcg cccgaagtct cgccgcccgc
    12241 gacgacgagc tggccgatgc cctgctcgac gaggccgcga gcgattgcag tgagcgcacg
    12301 ctcgacgagc gcaccggccc gttcgacacc gagccgcgac tgcacggcag ccaccgcgtc
    12361 cgcggccgcg gtcgatgaca cgagcagcgt ctcgtctgcg ggctggcgcg ccgaccatgc
    12421 gagcgcttcg cggaccatgt cgtcgttgga aagccgcagc gggtcgatcg tgaaggcgcg
    12481 gccgccacgt tcgacgaatg cacgcacctg gcgctgcgtc gccgtcgagc agctgccggc
    12541 gacgatcgcg cgccgcccgc gcggcggcgg cagcgcgctg gcgcggtccg acgcgacgag
    12601 cccgaagttc gccgggagcc cgatcgcgag tcccgaagcg gccgtgacga gcggccgctc
    12661 ggccagcgcc gccgcaagac ggcaaaggtc gtcgttctcg atcgcgtcga cgatcgccac
    12721 gccgatgccg tcgtgctcga gcgcggccag ccgcgcgcgg atcgcgtcgg tgccttcgcg
    12781 cacggcgcga tgttcgacga gaccgacgcg ccgccgcgat tgcgcctgca acacgcgcac
    12841 caggttggca tcgcgcatag gcgtcagagg atggtcctgc atgccgcttt cgttcagcaa
    12901 cgcgtggccg acgaagaggt gcccgttgta aaccgttcgg ccgttgtccg gaaacgccgg
    12961 tgtggcgacg gtgagcgccg cgccggcacg tcccgcgagc gcgtccatca gcgcgtcgat
    13021 cacggggccg atgttgccgt tgggcgtgct gtcgaatgtc gagcagtact tgaagtagaa
    13081 ctggcgcgcg ccatgcgcat cgagccactt gagcgccttg cgcgcgaggg cgacggcttc
    13141 gtcgacgggt gccgtacgcg acttcaacgc aacgacgatc gcgtcgatgc cctcgggcgg
    13201 cggcgcctcg ggcacgccga tcgtctgcat tgtgcgcatg ccgtggcggg cgaggttgtt
    13261 ggcgaggtcc gtcgcgccgg taaagtcgtc ggcgatcgca ccgagcagaa ggccgctcgg
    13321 gcggacggcg tcgcgcgggc gcgcacgctt cacgacgggc gctgccgcca tcgctaagcg
    13381 gcgacggcga cgccgcccgc cggcgtcggc ggtcgagcga cgcggcgcag gtgccagagc
    13441 tgcgaaagct cggaggcgca gcgcacgacg agcggtgcga gcgctgccac gcgctcgtcg
    13501 gtcatgcgca cgctcgggcc ggccacgctc accgtgccga gcgcgctgcc ctgcggtccc
    13561 gcacggatgg ccgcggcgat cgcgatgacg ccgggctctg cctcgctcat cgcgagcccg
    13621 tagccgcgcc gcatcgtcgc cttgagctcg cgcaggaacg cgtcgaggga acgcgtcgcc
    13681 ttgggcccgt agcgatcggc ctgcgcaaaa ccgccgttgg cctcgatgca ttcgatcgca
    13741 cgctcgaccc cgaaggtggc gagccacgcc ttgccgctcg cggtcaccga cagcggcacc
    13801 gtgttggtcg cgagcgacgg ctggtagacg agcccgctcg tcgctccttg cgcgtgggcg
    13861 acccatacga gcgaatggcc gtcgaccacg gcgagccgcg cgaactcgcg cgactctcgc
    13921 gcgaggcgat cgagcagcgg ctggcagacg tcggggatgc cggtcgcggc gtagaacttc
    13981 tgccccagcg tggcgaggcg catcgtgagc cgatagaagc tcgtgtcgat gtcctgctcg
    14041 gcccagccga gctcgcacag cgtggtcagg agccggtgcg cgccgctctt gggcaggccg
    14101 agcgcctcgg cgatgtcacc cagcggacgc gcgcttgcat cctcggcgag gagctcgatg
    14161 atcgcgaggc agcgttccgc gggtacgtgg gccatggcgt ggagcctgcg taccgtcagc
    14221 gaggtgtgtg caccggcgcg aggaaggaca tcagcgcgtc ggcctgaccg gcgtgcatgt
    14281 ccagcccagt gacgtatggc acgcgctcgc gctgggcgag ccgtatgatc ggtgtcggcg
    14341 cttcgcgaat cacgacgtcg ccgatcacgg catcggggcg aagacccgcg atgacgcccg
    14401 gcaggccatc ggcgtccgcc attcccaccg gcgtcgcgtt gacgatcatg tcgctgccgt
    14461 cgggcaccgc gtcggcgcag gcgaacgcca ccgatggaaa cgccgcgccg aaccgtgccg
    14521 ccagcatgtg cgcgcgcgct tcatcggcgt cgatcagcgc gatggatgca acgccggcgc
    14581 gcgccagcgc acaagcgatc gcgctgcccg caccaccggc accgaacagc gcaacgcggc
    14641 gagcctcgag acgcagtccc ttgcgctgcg cgccgtgcac gaagccttcg ccgtcgaaca
    14701 tgtcgcctgt ccaggtgcca tcggcctccc ggcgcagtgc gttgactgca ccgatgcacg
    14761 cggccgttgc gccaagcctg tcggcgaacg gcagcatgcg ccccttgaac ggcacggtga
    14821 cgacgacgcc gtcgagattg gcgatgcgga ggagtcccgg cacgatcgcg tcgaactgcg
    14881 cagcgggtac gtgtaccggg atcatgaccg catcgattcc ctcgcgcgcg aagcgctcgg
    14941 tgtacaccgc cggcgagcga acctgcgcca ccggatcacc gatgatcgcg aaaaggcgcg
    15001 tggcgccgcc gagtttcacc gcgtcgcttg tatgatcccc ccacgcttgc ggcttcgccg
    15061 ctgcgctgcc ccccgggggg gcgcaattcg cgccttgagg cggctctgca gcgctcatcg
    15121 cgaagcggcc gcgcgcgccg catgccattg cgccacgcgc gcagaacgat gttgccgcgg
    15181 tcgtgcgttg acgagcgcgt cgagtcccat cagtcccggg gcgaagatcc gctcgtccat
    15241 cgtcgccagc ttggcggcga tgcgcggccg gaaacccatt tgcgcgatga cgtcgcgctc
    15301 ggcgtccacg cccggcgcga tctcgatgag ctccagcccg tccgcaccga tgcggaacac
    15361 ggcgcgctcg gtgacgaaca gcgtcgtctg gccgcgttga cggccgattg ccgcgctgta
    15421 gcagacctgc tccagttctg gcacgaactt gcggtgacgg ccttcccggc ggatcaccgt
    15481 tcgaccctgt tcccattcga tgtcgagctc gcccgcggtc aacgtgccgc cgaacacgac
    15541 cgtgcgcgcg ttctggctga tattgatgaa accccccacc ccgatgatgc ggtcgccgaa
    15601 gcggctgacg ttgacattgc ccgccggatc gacttcggcg aacgacagga aagcgaggtc
    15661 gagaccgcct ccatcgtaga agtcgaactg cgcaggctgc tcgaccatcg ccgcgaagtt
    15721 ctgtccgcct ccggaatcgc ggcccgtgat cggggcgccg ccaatgattc cctgctcgtt
    15781 ggtgagcgtg accgtatcga ggaggccttc ctccgcgacg atcgtcgaaa gcccggtcga
    15841 aacgccggcc ccgaggttgc agatcgcgcc gggaaagagt tccatcgccg cgcggcgcac
    15901 gatcaccttg cgcgggccga actgcagcgg cttgatgtcg ccggtgggga tgcgcaactc
    15961 acccgagtag ctgggatcgt agtaggtgag ataggtctgg cgctgctcgg gttcgaccac
    16021 gacgaagtcc accaggatgc cgggaatgcg tacgctcttc gcggccagcg tattgcgccg
    16081 ggcgatccgc ttcacctgca cgacgacgat ccctcccgcg cgtcgcgtcg cctgcgccat
    16141 cgccagcatc tcgcccagca cggcctcttc ttccatggtg acgttgccgt cctcgtcggc
    16201 cgtcgtgccg cgcagaaacg cgacgctcac cggcaccggc ttgaacagca accattcctc
    16261 gcccgaaagc gtcacgacct cgacgaagtc gggcggggtg cgtgggctct gccgtccccc
    16321 ttgccgacgc ggatcgacga agctgtcgag accggtcctg gtgatgagcc caggccgccc
    16381 cgcagccatg tcgcgcatca gctgcgacaa caccccttgc ggcagcgtat aggcctcgat
    16441 cttgtcctcg gccgcaaggc gcacgagccc gggtgaatcg acgagtgcgc tcgtgatcgc
    16501 gcgtgccagc aggccctcgt gcgcgaggtg actcgcgccg agcgccgcgc gatcgccgac
    16561 accgacgacg ctgaccgacg tgaggccgcg cggcacgccc tccgccagaa accggcgctc
    16621 gacggcggcg agcaatgctt ccggcacagc gtggccgcca cccgaaccgc tgatgaggat
    16681 ggcgtcgcca tcctggatga gatgtgcgac gtcgtcggcc gagatctttt ccatgacgtg
    16741 tcagtggcgg gcgagtggcc gcccaggggt ggtggaagtc gccgctgcgg ccggcacctt
    16801 acgcgtgccc gagccggcag atctcgagca tgtagaccgc tccgtcgtcg cgcagtcgaa
    16861 ccgcacgttc gcgcaccgca accgggtcga cgtgcccgcg cgccgcctcc gcggccgcgg
    16921 cggtggtggc ctcgacaagg atcacccacg cggggacgtc gaactgccgg gtgcgcgatt
    16981 cggcggtgtc gatgaagctg cctccgcggt cgctcgcaaa aagatggcag gcgacgatgc
    17041 cggtgtgccc gagcgcgcgc ggaaacgcct cggtagccat atggctgaca aagccttctt
    17101 cggcggccgc gtcgccatcg aggcgcagtg cgagtacatg gccgccactg ctcgagcctt
    17161 gcgtgcaggc gacgcggcac acggcgcgtg acgttcggcg aaaataggga agcgtcgcac
    17221 gcgtgagcga cgtcggcgtg ttgagccgcg tgagatagtc ggacgaggtg agcacgtcga
    17281 tgccgcgcgc ctcgtacagc gtcatccact cgggcgaatg gccgggccgc gcgtagcggc
    17341 gaccacgccg gaatccgggg atggcgaggc gctcggggat atgttccttg tcgtgccagt
    17401 catagaactg ggcgcggcct tcggtggcaa cgtcgttcca gatcagcacc gctgcctcgc
    17461 cgagcagcgc cggtgcgcaa gccgcgtcat ccgcggcgcg gatcgcgggc gcggtggagg
    17521 cggccggcga atgccgcgaa cggctcatcg accctcgaac gccggatcgc gcttctcgaa
    17581 gaacgcggca acgccttcgt gcagatcgtc gctgtcgaag atgcgctgtt gcgccaccgt
    17641 cgaacgcgtc aacgcagcgt cgagagggcc gtcgtgggcg gcatcgacga gctccttcgc
    17701 aaggcggttc gacaacgggc cgcgcgacgc gatcgtcgcg gcgagctctc gcgcagcctc
    17761 cagcgccgat ccgtcactga cccggttgac gaggccccag gccagcgcga cgtcggccgt
    17821 gatcgtatcg ccggtgaaga gcaactcctt ggcgcgtcct ggccccacca gtcgtgcaag
    17881 gcgcagcgaa ccgttgccgg caaggccgcc gagacgcgat tccgtcaggc cgagcatcac
    17941 gccccggcgc gccacgcgaa gatcgcaggc cagcgcaagc tcgaggccac ccccgagcgc
    18001 ggaagcgtcg atcgccgcaa tcgtcggcac cgccaagcgt gcgagccggc ggacgaccat
    18061 gtcctcgaaa aggatcttct cttcactggc atttgcctgc aggtgcacga attcccggat
    18121 gtcgctgccg gcgcagaatg cgcgcgcgtt ctccgcatgc aggatcacgc agcgcagctc
    18181 gtgtgcagcc tcgagcgtcg ccagcacggc attgagcgcg cgcagaagcg ggcgcgtgac
    18241 caggttcagc gggccggccg tcaacgcgat ttccgctcgc gcaccctcgt gcgtcagtcc
    18301 gacgaggggg tgatccgcat gccgggtggt ggcgtcatcc attgtcatgg tgtcgccggt
    18361 gcggcgccga tgccggcgcc gtgcagcaca cccatttgct cgaacctgcg atagatgcga
    18421 ttgacgtacg cgccgaactg cggcgcgtcg cccagcacga ccggtcgtgg acgcgggagc
    18481 gcgacgcgaa tttcgtcgac gatctcgccg gggctcgggc tcatcacgaa gatgcggtcc
    18541 gacagcccca ccgcctcctc gacgctgtgc gtgatgaaga gcaccgtcgg acggcgccgc
    18601 agccatagcg cctcgaggtc catccgcacc tggagacgcg tcagcgcatc gagcgcgccg
    18661 aacggctcgt ccatcatgat gaccgacggg tcgtgcacca gcgcgcgcac gagcgaaacg
    18721 cgctggcgca tcccgcccga cagctgtcgc gggtatttct tcatggccgg cgtgaggcga
    18781 agctgctcga agagctcctt tgcgcgcgcc tcgatcggct tgcgcggcag gcggcgaata
    18841 tcggcctgca acatcacgtt gtcgatcgcc gtgcggaagt cgagcagcag atggtcctgg
    18901 aacgcgatgc cgacctcggt cagcgggcgt agcacgggct tgccgccgac cgcgatgtcc
    18961 ccctggctgc gcgacagcag ccccgcgacc atcagcatca aggtgctctt gccgcagccc
    19021 gacggcccca caacggacac gaattcgccc gcctcgatcg acatgctgat ctcgccgagc
    19081 gcacggaacg gctcgtcgct gtcggcgccg aaggttttcg acacgccgca aagctcgatc
    19141 ggcacgccgg agcgcacggc cgattcgctc gcacgcacat cggccgcgac gctcatcccg
    19201 atcctccggc cgacgccgac aacgccgcga gcggcacctt ggacacgagc agcgccttgc
    19261 gtccccgctg gacgaccgcg cgctcctggt ttttcaccgc gacatcgaac gtcgcgattg
    19321 cctgggttgg ccgagagcgg ctgtcgcgaa gctcgacgat cgcgtagtcg acgaagatcg
    19381 tgtcgccgac gaagatcggg ccgacgaagc gccagtcgct tatgccgagg aaagcgatcg
    19441 ccacttcgcg cagctcgccg gtgcgcgccc acatgaggcc gtgagcgtac gcgaggccca
    19501 gcatcccatg cgcgacgcgc cggccgaact cgctggcctt cgcgtagacg tcgctcgtat
    19561 gcagctccga atagtcgccg gtgagcccgg cgaacgcgac gagatcggca tcggtgatcg
    19621 tgcgccccgg gctgcggaac gccaacccca cgtgaaggtc ggcgtaacgc aggctggtgc
    19681 gaatggtctc gctcaccggg cgagcctcgt cggtgagatg gcgtgcacat cgcgcacaat
    19741 tacttgcatg cgcgcattcc ggacgccgcc ggcagaaact cgtcggtgta gtacgacttc
    19801 gggtcctggc ccgccggcag cagcttcact tctgacaaga ggtcctgcgt cttcacccac
    19861 agcgcgtcct cggccttgcc gatgtacttt gcgccgccac tgcagaagag cggcgtgatc
    19921 gccgcgagct ctgcagtcgc caccttctcg ttcatttccg ggaacacctg cttcaggtcc
    19981 ttgatcgcct tgtccggatg atcgagggcg aagctccagc ccttcaagct cgccgcgatg
    20041 aagttgcgga tgacggcggg gttgtccttc atgtactgcg tattggcgaa aatcgacgtg
    20101 ctgaccgtgg gcacgccata gtcggcaaag cggtaattgt cgagctccgc accctgcgcc
    20161 tcgagctgga tctggtaggc gtccatcgag cccaggatcg cgtcgacctg tccctgcagc
    20221 aacgatggca ccatcgatgt catcggcatg ttgatcaggt tgacgtcggt ttcggtcagg
    20281 tggttggcct tgaggaacag cggcagcatc gtcgtctgcg acgaccccga cggcacgccg
    20341 accttcttgc ccttcaggtc cgcgatcgac ttgatgccgg tcttcttgag cgccgatact
    20401 tcgttgggat tcgactgata gatcgtggac acgatcttca tcggcgcgcc cttcgcgatc
    20461 agctggctca cggccacggc atcggcatac gcgagctgcg cgcgcccgct ggcgacgagc
    20521 tgcgcggtat tgcccgagcc gttgccttgc accagcgtga cgtcgagccc ggcgtccttg
    20581 tagtagccct ccgcgacggc ggcggcgaac ccggcattgg ggccaccggc gtaccagttg
    20641 agctggaacg tcactggcgt cgccgccccg gcgtcgaaca tcgttacgag tccggcgacg
    20701 gcgagaccgc cgacgagcaa ccgcttcacg cacttttgca tggaactcct cctttggatg
    20761 gcgctggtca tggtgctggc cggacgcgca gcgacattcg ccgctgcgcg gtcaggtgtt
    20821 cttcgagcgc tgccacggcg tcatcgccca ttcgctcaat tccaccgcgt agttgatcgc
    20881 gatgccgatc gccgtgagca ccaccagcac cgcgaatacg agttcgatgt ccatcgtgcc
    20941 ggtgcccttg agcagcacgt agccgagccc gcggttggcg ccgacgaact ccgcgacgat
    21001 ggcggcggtc gcggcgatcg tcgccgacgt cttgatgccc gagaagatta ccggcagggc
    21061 ggccgggaaa cgcagatagc ggaatgtctg ccaacggttc gcgcccatcg attgcgtcaa
    21121 atagagcagg cgctcgtcga ggatctggaa gccgctgatg ctggccatca gcaacggaaa
    21181 gaacgtcatc agcaccgtga gcagcacctt cgattccatg ccgaaaccga gccagacgag
    21241 aaacagcggc gcgaccgcga tcttgggcac gagctgcatc aacatcactg gcgggtagac
    21301 gatctgcttc gccgtgcgcg atagcgcgat gagaaggccg agcggaatcg cggtgacgac
    21361 ggtcagaccg aagccgagca ggatctcggt cagcgtcgtc accgaatgcg accacagcat
    21421 gggtgcgtcc gagatgatgc gcgagaaaat gctgccgggg ccgggcagca ggtacgcggg
    21481 aatcttgaac gcggcgactg ccgcggccca cacacccacc atggcgacga acgtgagcgg
    21541 cggcagcagg ctccacgcaa gattgcgcag gcggcgacgg tgcgacgagt gcgcacccgc
    21601 ggtcggcgcc ggcggcaacg ccgtctgcca gtcgggctga cgctcgatgc ccggcgacac
    21661 gtcgtgcaac gcgtggtttg ccatcgctcc tcggctcgtc gatcggaagg cgccaaggtc
    21721 gcgcgccgat cgcgacgggt ggacggcggc cttgcagtcc gcgcaccggc tcgcgctccc
    21781 gtcgggcttg gaaacgctcc tgccaaaatg gaatgctatt ccaacttgcg cgggcgcgtc
    21841 aacgaaccgt cgcaaccgcg cgaaattcgc gcgatggcgt tatgtttatg cgggcgggga
    21901 cacgcggcga gttcaacaac ctcggcgcgt tcgtttgtcg ctcgatctgc cgtgctttcg
    21961 cgcaccacac tacgtagcgc gtgaggaaga cgactgcgag atcgcgctcg gcgcgcgcgg
    22021 atcgtccctc agacggacct cgcgcccggc cgcgcgagtc ggaaagcgcg gccggtcaag
    22081 cggtcgtcgc gctgccacga atcgagcgta cagtcgctcg ttgccaatca ccaccgaggg
    22141 gaagcgtatg aaccggagag cgaatgcgcg gggcggtcgc ccgtccttga gctttgtcgt
    22201 cgcggccctc gggctcgtgt ccacggtcgc cgtcgcccaa aacgacgcat cgcttcgtgc
    22261 cggtgctaac gcgctgctgg cgatcgacca gcatcgcgcg acggtgatcg agcggatcgt
    22321 ttcgcaatgg ggtgattcgc ttgcgccagc gggaacagtg ctcgacatgg ccgaactgcg
    22381 cacatcgctc gcgaccttgc gcgctgacca cctgttggcc gccagtcttg ccgggagtgc
    22441 cgacggcgtt cgcgacgtgc tggcgactgc gctggcgtcc gtgccgaagc ccggcgacgg
    22501 acgagcgcaa acaaaggcgc tcggcgagat tgccgatgat gtcgtctata cgccagtgac
    22561 gccgtgccgc ttagtcgaaa cgcgcggcac gttcgcggcg gtctaccaag gtggcggggc
    22621 gttcagcgga ggcaccatcc gcacctatac cttgcagggc ggcaacggcg tatgtctcgc
    22681 ccaactgccg gagggcatcg ccccgaccgc ggtgcaattg caggtgttcg gcatcccgat
    22741 caacggcgcg gcgagcggcg acattgaaat cctgccgcag ggagcgacgt tcggcacgac
    22801 ggcgaccgag gtctacgtcg gtaacgtggc gttcaatacc gtgtcgacaa cggcgaaggt
    22861 gaacgttgcg aacaatcaga tcgccgtgca ggtaagaggc ggtggagcga acctcgccat
    22921 ggatgtggtg ggttatttca agcgaccggg taattacggc ggagtgcaga cgatcggtgg
    22981 cttggcatcg gatgcaacag tcagcggcgg gttcaacaat accgccaccg gcgcaatcag
    23041 tgtcgtcgcc ggggggcagt caaatactgc aagtggccac gccagcgcag ttggcggggg
    23101 ggagttcaac acggcgagcg gcatcgacag tacggtgggc ggtggggaag gcaacgtcgt
    23161 aagtgggtcg aatagcatgg tcccgggagg attcaacaac accgccagcg gcgtgaacag
    23221 cttcgccgcg gggtcttctg cagtggcc
//
LOCUS       DASSVX010000082         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_802749, whole genome shotgun sequence.
ACCESSION   DASSVX010000082 DASSVX010000000
VERSION     DASSVX010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802749"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFO53_03825"
     CDS             complement(<1..535)
                     /locus_tag="VFO53_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HET8875436.1"
                     /translation="MISRNCIALALSLGVTFAHADAPGDFASRQALATPGDKAFYRIE
                     LPDSVYDAVTRADLGDLRVFNADGAPVPYAFIPRPAPVVTPTPHRPLALFPLTVDTTA
                     PNAGDVAIKVRRDATGTIVDVRARDGTPRSGSRVVAYLIDTGDDTVPLTALIAGLPDM
                     GAINGRVRLDASDDLDRW"
     gene            complement(532..>2638)
                     /locus_tag="VFO53_03830"
     CDS             complement(532..>2638)
                     /locus_tag="VFO53_03830"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HET8875437.1"
                     /translation="IGILYLTVFAALRLYQLLPPTAAFGLLLWIAAMSAFLAVRQDAM
                     ALAVLGIVGGFAAPLLTSTGGGSHVLLFGYFAVLNAAIFAIAWFKAWRLLNIVGFACT
                     VVIGTLWGVTRYRPEDFATTEPFLILFFMFYVGIATLYALRQSIAVRNYVDGTLVFGT
                     PLVAAGLQSALVRPYEYAMAWSALAASALYLVLARMLYARHRNDLRLLVEAFLALGVV
                     FATLAVPLALDSRLTSATWALEGAALVFVGVRQQRLAPRVFGLALQLAAGVAFGLGFG
                     IWTARLVGGSLPVLNSDCIGALLVALGGLISARVMSGAAITGSERLLVPVVFGWGVLW
                     WLGAGVREIERFVAPDARAAALVMFVTASAALFDAVAQRLQWPLARVPALALPPALLL
                     LAAYRVVTPHGMDAHLFAGLGAIAWPLAVVVYVLLLRRFERASVAPALLDWLHGLLGW
                     LVTLIVASELAWLARDNLSGSAWRLVPWGLVPALVVLAIGWLTSRAAWPFAPRRRAWL
                     VIGAIPLLVASLAWTLYANVASAGDAAPLPFVPLLNPLDLTQGLVFVAIATWLSQVRS
                     VEANRLGAVSPAALGAAASVLLLFWLTFATLRTLHHFADVPWALSAMWASRIVQATLA
                     IVWSVAALAAMVVANRRRYRAAWVAGAALLAVVVAKLFFVDLSQVGGVERIVSFMGVG
                     VLLLIIGYMAPVPPRRELA"
BASE COUNT          553 a          912 c          872 g          301 t
ORIGIN      
        1 gccagcgatc gagatcgtcg ctcgcatcga gccgaacgcg cccattgatg gcgcccatgt
       61 cgggtaatcc cgcgatcagc gccgtgagcg ggaccgtgtc gtcaccggta tcgatcaggt
      121 aggcaacgac gcgcgagccg cttcgcggcg tgccgtcgcg cgcgcgaacg tcgacgatcg
      181 tgcccgttgc atcgcgccga accttgatcg cgacatcccc cgcgttcggt gcagtggtat
      241 ccaccgtcaa tggaaacagc gcgagcgggc ggtgaggtgt tggtgtcacg acgggtgcgg
      301 gccgcggaat gaatgcgtaa ggcaccggcg caccgtcggc gttgaagacg cgcaggtcgc
      361 caagatccgc gcgcgtcact gcgtcgtaga ccgaatcggg caattcgatg cggtagaacg
      421 ccttgtcgcc cggcgtggcg agcgcttggc gcgacgcgaa atcgcccggc gcatcggcgt
      481 gcgcgaacgt cacgccgagc gacaacgcca gcgcgatgca atttcgcgag atcacgcgag
      541 ctcccgccgt ggcggcacgg gtgccatgta gccgatgatg agcagcagca cgccaacccc
      601 catgaacgac acaatccgct cgacgccgcc cacctgcgag aggtcgacga agaacagctt
      661 ggcgacgacg acggcgagca gcgccgcgcc cgcgacccac gcggcgcgat agcggcgccg
      721 gtttgcgacg accatcgctg cgagcgccgc cacgctccag acgatcgcca gcgtcgcctg
      781 cacgatacgc gatgcccaca tggctgacag cgcccacggc acgtcggcaa agtgatgcag
      841 cgttcgcagc gtcgcgaacg tcagccagaa cagcagcagg accgaggcgg ccgcaccgag
      901 cgccgcgggc gacacggcgc cgagccgatt ggcttcgaca ctgcgcacct gcgaaagcca
      961 cgtcgcgatc gcgacgaaga cgagaccctg tgtcaggtcc agcggattga gcaatggcac
     1021 gaatggcagc ggcgctgcgt ccccggcgct cgcgacattg gcgtacagcg tccaggcgag
     1081 ggatgcgacc agcagcggaa tcgcgccgat gacgagccac gcgcgccgtc gcggagcgaa
     1141 tggccacgca gcgcgcgacg tcagccagcc aatcgcgagc acgacgagcg ccggcacgag
     1201 tccccatggc acgaggcgcc acgcactgcc gctcaggttg tcgcgggcaa gccacgccaa
     1261 ttcgctcgca acgatcagcg tcacgagcca gccgagcagg ccgtgaagcc agtcgagcaa
     1321 cgccggcgcg accgatgcgc gctcgaatct tcgcagcagc aagacgtaaa cgacgaccgc
     1381 gagcggccat gcgattgcac caagaccagc gaagagatgc gcgtccatcc catgcggcgt
     1441 gacgacccgg tacgcagcga gcaacagcaa cgccggcggc agcgcgagcg caggtacgcg
     1501 tgcgagcggc cactggagtc gctgcgcgac ggcgtcgaac aatgcggccg atgcggtgac
     1561 gaacatcacg agtgccgcgg cccgtgcatc gggcgcgacg aagcgctcga tctcgcgcac
     1621 gccggcaccg agccaccaaa gcacgcccca tccgaacacg accggcacga gcaaacgctc
     1681 gctgccggtg atcgctgcgc cgctcatcac gcgcgcgctg atgaggccac cgagcgccac
     1741 gagcaacgcc ccgatgcaat cgctgttcag caccggcagc gagccgccga cgagccgcgc
     1801 cgtccagatg ccgaatccca gtccgaatgc gacgcccgcc gcgagctgca gcgcgaggcc
     1861 gaacacgcgc ggcgcaagcc gttgctgccg cacgccgacg aagacgagcg cagcgccctc
     1921 gagcgcccac gtcgccgagg tgagccgcga atcgagcgcg agcggaacgg ccaacgtggc
     1981 aaagacgacg ccgagcgcca ggaaagcttc gacgagcagt cgcaggtcat tgcgatgccg
     2041 cgcatacagc atccgcgcga gcaccaggta gagtgccgaa gctgccagcg cgctccacgc
     2101 catcgcgtat tcataggggc gaacgagtgc gctctgcagg cccgcggcaa cgagcggcgt
     2161 gccgaagacg agcgtgccgt cgacgtagtt ccgcaccgcg atcgattgac gcagcgcgta
     2221 tagcgtcgca atgccgacat agaacatgaa gaacaggatc aggaatggct ccgtcgtcgc
     2281 gaagtcctcc ggccgatagc gagtgacgcc ccacaaggtg ccaatgacga ccgtgcacgc
     2341 aaagccgacg atgttcagga gccgccatgc cttgaaccac gcgatcgcga aaatcgccgc
     2401 attgagcacg gcgaaatagc cgaacagcag cacgtgactg ccgccaccgg tcgaagtcag
     2461 aaggggcgct gcgaagccgc cgacgatgcc gagcaccgcg agcgccatcg cgtcctgccg
     2521 cacggcgagg aaggccgaca tcgcggcgat ccacaacagc agtccgaacg ctgccgtcgg
     2581 cggcagcaac tggtaaagcc gtaacgccgc aaacaccgtc aaatacagaa tgccgatc
//
LOCUS       DASSVX010000083         4908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1552825, whole genome shotgun sequence.
ACCESSION   DASSVX010000083 DASSVX010000000
VERSION     DASSVX010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552825"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFO53_03835"
     CDS             complement(<1..191)
                     /locus_tag="VFO53_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875438.1"
                     /translation="MDVPTLQRGDAAARMRFPLARKAFAGLTLNAVAIIALLLVIRSV
                     SASVDLIVAIKSGREALEFL"
     regulatory      352..524
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            528..1091
                     /locus_tag="VFO53_03840"
     CDS             528..1091
                     /locus_tag="VFO53_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HET8875439.1"
                     /translation="MEAFLVSTGVVALAEIGDKTQLLAFILAARFRRPAPIIGAILVA
                     TLANHALAGAVGAWITSLLGPQTLRTVLGLSFIAMAIWTLIPDRLDAGDAKLPHAGVF
                     ATTLVAFFLAEMGDKTQIATVALAARYHAFAAVVAGTTLGMMIANVPAVLVGGRLAHR
                     LPIRLVHAIAAAIFATLGVIALTGIGA"
     gene            1185..2093
                     /locus_tag="VFO53_03845"
     CDS             1185..2093
                     /locus_tag="VFO53_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875440.1"
                     /translation="MSLTLTMTGLSKTYPNGVRALSDVSLSIPAGMFGLLGPNGAGKS
                     TLMRTLATLQDADAGTATLGDMDVLRDKAAVRRILGYLPQDFGVYPKVSAEELLDHLA
                     RLKGLSDKRARHDVVDALLRQTNLHDVRKKALGGFSGGMRQRFGIAQALLGNPRLIIV
                     DEPTAGLDPEERVRFHNLLAEIGENVVVILSTHIVSDVSDLCAQMAIIDKGRVLLTGR
                     PSDLTRRLDGRVWQKAIARADLAATKAMYPVISTRLVAGRTLVNVVSDATPGEGFAPV
                     AATLEDVYFAAIAGRLDTPARALEPA"
     gene            2093..>4908
                     /locus_tag="VFO53_03850"
     CDS             2093..>4908
                     /locus_tag="VFO53_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875441.1"
                     /translation="MRAFAAVAGFEFRTRVARISTWVYFAIFGALAMLWMAAAGGALR
                     NAAVSFGSGKIWIDSPYALAQMFALLGMASLTVIGAVMGRAVQQDFEYRTEAFFFTAP
                     IRKVDFLAGRFAGAVAVLLVILSSIPIGSALGLLLPGIDADRVGPVRIAAWLLPYATT
                     LLPNVIVLGGFFFCLAALTRRMLPVYVASVVLLIGYLAAQGLLRDMENKLLAVMLDPF
                     GSTALSKLTEYWPISERNTRLVPLAGFLLWNRVLWLALAAAALALTAWRFRFTQPSMA
                     PAKRRGRESDPVPAASVVLPSSTLHPPRITPDEVRPWRLLPGMVWLNFRETVKNVYFA
                     VIALAGVLFCITTSTTAGSIFGTVTWPVTYQMLEIVSGTFAIFMLVIVTFYAGELAWR
                     ERESRIDQIHDALPIPTWLPFAAKLLALMLVPVVLQALLMVTGLGIQTAKGYHHYEPA
                     LYLHWLFGIELVDYWLVCVLALTVHSLVNQKYLGHFVMIVYFIAVSFASLLGLEHNLY
                     KYAANGNFTYSDMNGFGPFLARVRFFQAYWAAGAVLLAVAAYLFWPRGTTVDWRARMM
                     AARQRATPRVAGLTAFAAVVMAGLGAYIFYNTNVLNPYVTTHDREARQANYEKRYKAL
                     EAAAQPKITDVSVAVDLYPAEQRVRMKGHYLLENRTSRPIAAIHLTFLRGDQLVIHTL
                     EFATPTTLVTDDMTTGLRSYRLASPLQPKGRLELDFDLEVPTHGFTNGGATTDVVANG
                     SFVNGQSVLPFIGYQERGELVSDSDRKKFGLKPKERMRARDDPTGLGQNAIATDADFI
                     DFDATVGTDADQWAIAPGYLQREWTERGRHYFAYRMDAPILDFFAVQSARYAVAKDRW
                     HDVAIEVYYQPGHEYNLERMIAATKAGLDYFTANFGPYQYRQFRIIEFPRYAAFAQSF
                     PNTVPYSEGIGFIARVRDADPEDI"
BASE COUNT          756 a         1675 c         1596 g          881 t
ORIGIN      
        1 aggaactcga gcgcctcgcg gccgctcttg atcgccacga tcagatcgac ggacgcgctg
       61 acgcttcgga ttacgagcag cagcgcgatg atggcgacgg cgttgagcgt caggcccgcg
      121 aacgccttgc gtgcgagcgg aaagcgcatc cgcgcggccg cgtcgccgcg ctggagggtg
      181 ggtacatcca acgtggcggc gagtcgcggc atgggcgaaa attggcagcc tccggcgagc
      241 gaggtcgccg ccgggagatg gcgtcgcgag gataggcgga agcggcccac cgaggcaatc
      301 gatgcccgac gaagccgcgg aatggcggtg cgaatgcgtt gccgtatact tcgcgcagtc
      361 actggggagt agccgccctt cctcttacgg ggcttgcgtc aacacacttg gccgatcggc
      421 catggcgcaa gcggttgcct ggcctggcga gacctttgac cacgccgcct tcgcatggcc
      481 ggaggagcgg cgcggtcatt cgtccgcatc ccggccaggg agtcccgttg gaagccttcc
      541 tcgtctcgac cggtgtcgtc gcccttgccg aaatcggcga caagacacag ctcctcgcgt
      601 tcatcctcgc tgcccgcttt cgcaggccgg cgccgatcat cggcgcaatc ctggtggcga
      661 cgctcgccaa ccatgcgctc gccggtgccg tcggcgcatg gatcacgtcg ctgctcgggc
      721 cgcaaacgtt gcgcaccgtt ctcgggctct cgttcatcgc catggcaatc tggacgctca
      781 tccccgatcg cctcgatgcc ggcgacgcga agctcccgca tgccggcgtg ttcgcgacga
      841 cgctcgtcgc cttcttcctc gccgagatgg gcgacaagac gcagatcgcc accgtggcgc
      901 tcgccgcgcg ctatcacgcg ttcgccgcgg tggtggcggg aacgaccctc ggaatgatga
      961 tcgcgaacgt gcccgcggtg ctggtcggcg gacggctcgc ccatcggctg ccgatcaggc
     1021 tcgttcatgc catcgcggcg gcgatctttg ccacgctcgg cgtcatcgcg ctgaccggca
     1081 tcggtgcgtg agcgctcttt ttcacgcgat ccgaatccgg ggcgcgcatt ggtaacatct
     1141 cacgctcgct cgccacgacg aggggcgctc cccgaggatt tgcgatgtcg ctgacgctca
     1201 cgatgaccgg tctttcgaag acgtatccga acggggtgcg cgcgctgtcg gacgtctcgc
     1261 tttccattcc cgccggcatg ttcgggctgc tcggtcccaa cggcgcgggc aagtcgacgc
     1321 tgatgcgcac gctggcgacg ctgcaggacg ccgatgcggg gacagcgacc ctcggcgata
     1381 tggacgtgtt gcgcgacaag gccgccgtgc gccgcatcct cggctacctg ccgcaggatt
     1441 tcggcgtgta cccgaaggtg tccgcggagg agctgctcga ccacctggcg cggctcaagg
     1501 ggctgtcgga caagcgggca cgccacgacg tcgtcgacgc gcttctgcgg cagaccaatc
     1561 tgcacgacgt gcgcaagaag gcgctcggcg gattctcggg cggcatgcgc cagcgcttcg
     1621 gcatcgcgca ggcgctgctc ggcaatccgc ggctcatcat cgtcgacgag ccgaccgccg
     1681 gactcgaccc ggaagagcgc gtgcgcttcc acaacctgct cgcggaaatc ggcgagaacg
     1741 tcgtcgtgat cctgtcgacg cacatcgtgt cggacgtttc ggatctttgc gcgcagatgg
     1801 cgatcatcga caagggccgc gtcctgctga ccgggcgtcc ttccgatctc acgcgacgtc
     1861 tcgacggacg cgtctggcaa aaagcgatcg cgcgcgccga ccttgcggca acgaaggcga
     1921 tgtatccggt catctccacg cggctcgtcg cgggtcgcac gctcgtcaac gtcgtgagtg
     1981 acgcaacgcc cggcgaaggc ttcgcgcccg tggcggcaac gctcgaggat gtctacttcg
     2041 ccgcgatcgc cgggcggctc gacacgccgg cacgggcgct cgagccggcg tgatgcgcgc
     2101 attcgcggcc gttgcggggt tcgaatttcg cacccgcgtt gcacgcatct cgacctgggt
     2161 ctatttcgcg atcttcggcg cccttgcgat gctgtggatg gccgccgcgg gcggcgcact
     2221 gcgcaacgcc gcggtgtcgt tcggcagcgg caagatctgg atcgactctc cgtacgcgct
     2281 cgcacagatg ttcgcgttgc tcggcatggc gtcgctgacc gtgatcggcg cggtgatggg
     2341 acgtgcggtg cagcaggatt tcgagtaccg caccgaggcg ttcttcttca ccgcaccgat
     2401 ccgcaaggtc gacttcctcg ccggccgctt cgccggcgcc gtggcggtgc tcctggtgat
     2461 cctgtccagc attccgatcg gcagcgcgct cggcctgctc ctgcccggca tcgacgccga
     2521 ccgcgtcgga ccggtgcgca tcgcagcctg gctgcttccc tacgcaacga cgctgctgcc
     2581 gaacgtcatc gtcctcggcg gctttttctt ctgcctcgcc gcgctgacgc gccggatgct
     2641 gccggtgtac gtcgcgagcg tcgtgcttct catcggctat ctcgccgcgc agggattgct
     2701 gcgcgacatg gagaacaagc tcctggcggt catgctcgat ccgttcggct cgaccgcgct
     2761 gtcgaagctg accgaatact ggccgatcag cgagcgcaat acgcggctcg tgccgctcgc
     2821 aggcttcctg ttgtggaacc gcgtgctgtg gttggcactc gccgctgcgg ccctcgcgct
     2881 cacggcctgg cgctttcgct tcacgcagcc gtcgatggcg ccggcaaaac gccgcgggcg
     2941 cgaatcggat ccggtgccgg cggcgtcggt ggtgctgcct tcatcgacgc tgcatccgcc
     3001 gcgcatcacg ccggacgagg ttcgcccgtg gcggctgttg ccgggcatgg tctggctcaa
     3061 ctttcgcgaa acggtgaaga acgtctactt cgccgtcatc gcgctggccg gcgtgctgtt
     3121 ctgcatcacc accagcacga cggcgggcag catcttcgga acggtcacct ggccggtgac
     3181 ctatcagatg ctggaaatcg tgtcgggaac gttcgcgata ttcatgctcg tgatcgtgac
     3241 gttctacgcc ggggagctcg catggcgcga gcgcgaatcg cgcatcgacc agatccacga
     3301 cgcgctgccg attccgacgt ggctgccgtt cgcggcgaag cttctcgcgt tgatgctcgt
     3361 ccccgtcgtg ctgcaggcgc tgctgatggt caccggcctc ggcatccaga ccgcgaaggg
     3421 ctaccaccat tacgagccgg cgctctacct gcactggctc ttcggcatcg agctcgtcga
     3481 ctactggctc gtgtgcgtgc tggcgctgac cgtgcactcg ctcgtcaacc agaagtacct
     3541 cggccatttc gtcatgatcg tgtacttcat cgccgtttcg ttcgcaagcc tgctcggcct
     3601 ggagcacaac ctgtacaagt acgcggcgaa cggcaacttc acctactcgg acatgaacgg
     3661 cttcgggcct ttcctcgcgc gcgtgcgctt cttccaggcg tactgggcgg ccggcgcggt
     3721 gctgctcgcg gtggcggcgt acctgttctg gccgcgcggc acgaccgtcg actggcgcgc
     3781 gcggatgatg gcggcgcgcc agcgcgccac gccgcgggtt gccgggctga ccgcgttcgc
     3841 cgccgtcgtc atggcagggc tcggcgcgta cattttctac aacacgaacg tgctcaatcc
     3901 gtacgtgacg acgcacgatc gcgaggcgcg ccaggcgaac tacgaaaagc gctacaaggc
     3961 gctcgaagcg gcggcgcagc cgaagatcac cgacgtgagc gtggccgtcg atctgtatcc
     4021 cgccgagcag cgcgtacgca tgaaaggcca ttaccttctc gagaatcgga cgtcgcggcc
     4081 gattgctgca attcatctga cgttcctccg cggcgaccag ctcgtgattc acacgctcga
     4141 gttcgcgaca ccgacgacac tcgtcaccga cgacatgacc accggcctgc gcagctaccg
     4201 tctcgcctcg ccattgcagc cgaagggcag gctcgagctc gacttcgatc tcgaggtccc
     4261 gacgcacggt ttcaccaacg gcggtgcgac caccgatgtg gtcgccaacg gctcgttcgt
     4321 caacggccag tcggttctgc cattcatcgg ctatcaggag cgcggcgagc tcgtcagcga
     4381 cagcgaccgc aagaagttcg gcctcaagcc gaaggagcgg atgcgcgccc gcgacgatcc
     4441 gacaggcctc ggacagaacg caatcgcgac cgacgccgac ttcatcgatt tcgacgcgac
     4501 ggtcggcacc gacgccgatc aatgggcgat cgcgcccggc tacctgcagc gcgagtggac
     4561 cgagcggggg cgccactatt tcgcgtacag gatggacgcg ccgatcctcg acttcttcgc
     4621 cgtccagtcc gcgcgctacg ccgtcgcgaa ggatcgctgg cacgatgtcg cgatcgaggt
     4681 ctattaccag cccggccacg agtacaacct cgagcggatg atcgcggcga cgaaggcggg
     4741 tctcgactac ttcaccgcga acttcggccc gtatcagtat cggcagttcc gcatcatcga
     4801 gttcccgcgc tacgcggcgt tcgcgcaatc gtttccgaac acggtgccct actcggaggg
     4861 catcggcttc atcgcccgcg ttcgcgacgc cgatccggag gacatcga
//
LOCUS       DASSVX010000084         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_536042, whole genome shotgun sequence.
ACCESSION   DASSVX010000084 DASSVX010000000
VERSION     DASSVX010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536042"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="VFO53_03855"
     CDS             <1..1257
                     /locus_tag="VFO53_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET8875442.1"
                     /translation="ASEWCDRTLLARIHRYTVNRLRQEVEPVTTQDFMRFLFRWQHVA
                     PDEQRQGPDALDAIVTQLQGFEAPAASWESELLPARLTHYEFTWLDDLCLSGRVVWTR
                     LAPPAPSSAAGSANVIRTTPVTLLPRRTAPLWTRAAPMPAAAPTQASSRAQAVVDFLQ
                     AHGASFYDEIVDGTRLLRTQVEDALAELVALGRVTSDSFGGLRALLTPSQKRKKAGAG
                     RRARRTALLGVEDAGRWALTRRPIPASTPGWTRADPAHDSETIEHIAKALLRRYGVIA
                     WKILQREATWLPPWRELVRVLRRLEARGDIRGGRFVASVTGEQFASPEAVAMLRETRR
                     AAPTGRFVSVSGADPLNLVGTVLPGSKVPALTANRILYRDGAPVATLVGGELQWLQSL
                     DATDARVAEHVLLKRTPGSPLLAYLR"
     gene            1685..2857
                     /gene="opgC"
                     /locus_tag="VFO53_03860"
     CDS             1685..2857
                     /gene="opgC"
                     /locus_tag="VFO53_03860"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HET8875443.1"
                     /translation="MERQTELDWLRGLMLVLMTITHLPTWFSSHVGQPFGFVSAAEGF
                     VFLSAYLVGSVYSGTALRHGFVAMRNAIWRRAAKVYAAQVALLLLLFWVLLPYATSQG
                     AHAITDLASFYAERPHTALVSALALAYNPPLLDILPMYVLFMLASPLLLEYGWQRGWG
                     PLIGLSAAMWLLSQHDGGRDIYGYFAGIVGWPVPYNQTGAFAFLAWQLLWVAGLRAGA
                     LHVQEAQGGEPRTAWSRPVLWTATALAVGFFAWRHITGQVPFGDHGSLNALFDKWHLG
                     PMRLLNFAVLAILAVHGRRWLVAVAQRSSIATLGRASLTVFAAHLVLCLTLLASGETA
                     LDSQHVSLLDGTLLAGTLVTLYAIARATLGGSKLIARWTTVAPPKATADVSARAVR"
     gene            complement(2835..>3534)
                     /locus_tag="VFO53_03865"
     CDS             complement(2835..>3534)
                     /locus_tag="VFO53_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET8875444.1"
                     /translation="MTFKSVSTFACALFSALTFAAHAAGAPSTPAADAPKGSPWEQAF
                     AAFADDDASHPHPAGGVLFVGSSSIRLWSDLQEQFKDLPVVINRGFGGSELADCVKNL
                     NRLVIRYRPSTVLVYAGDNDLAAGTTPAEVLHRFAAFVDGVHHELPDTRIAYISIKPS
                     PLRIRLMPQIKETNALIRDYAAGLDKVAFIDVYTPMLDSAGKPRAELFRADSLHLNAD
                     GYALWKRVIGPHVH"
BASE COUNT          524 a         1151 c         1228 g          631 t
ORIGIN      
        1 gccagcgaat ggtgcgaccg tacgctgctt gcacgcatcc atcgctacac ggtgaaccgg
       61 ctgcggcagg aggtggagcc ggtgacgaca caggacttca tgcgcttcct gtttcgctgg
      121 cagcacgtgg cgcccgacga gcagcgccag gggccggacg cgctcgatgc gatcgtcacg
      181 caactgcaag gcttcgaggc accggctgct tcgtgggagt ccgagctgtt gcccgcgcgc
      241 cttacgcact acgaattcac gtggctcgac gacttgtgcc tgtcgggacg cgtcgtgtgg
      301 acgcggctag caccccctgc gccgagcagt gccgcgggca gcgcgaatgt aatccggacg
      361 acgccggtca cgttgctgcc tcgtcgtacc gcgccgctat ggacgcgcgc cgcgccgatg
      421 ccggctgccg cgccgacgca ggcgagctcg cgggcgcagg cggtcgtcga tttcctgcag
      481 gcgcacggcg catcgttcta cgacgagatc gtcgacggca cgcgccttct ccgcacacag
      541 gtcgaggacg cgctcgccga gctcgtcgca ctcgggcgtg tgacgtcgga cagctttggc
      601 ggcttgcggg cgcttttgac gccctcgcag aagcgcaaga aagcaggagc ggggcgccgt
      661 gcgcggcgca cggcgctcct cggtgtcgag gacgccggac gctgggcact cacgcgtcgt
      721 ccgataccgg cctcgacgcc cgggtggacg cgcgcggatc cggcacacga cagcgagacc
      781 atcgagcaca tcgcaaaggc gctgctgcgc cgctatggtg tcatcgcgtg gaagatcctg
      841 cagcgcgagg caacgtggct tcctccatgg cgcgagctcg tgcgcgttct gcgcaggctc
      901 gaagcgcgcg gcgacattcg cggaggacgc ttcgtcgcaa gcgtgaccgg cgagcaattc
      961 gcatctcccg aggccgttgc gatgctgcgc gaaacgcggc gtgcagcgcc gacgggacga
     1021 ttcgtctcgg tcagtggcgc cgacccgctc aatctcgtcg gcacggtgtt gccggggtcg
     1081 aaggttccgg cgttgacggc gaatcgaatc ctctatcgcg acggcgcgcc ggtggcgaca
     1141 cttgtcggcg gtgagctgca gtggttgcag tcgctggacg ccacggatgc tcgcgtcgcc
     1201 gagcacgtgc tgctgaaacg cacgccagga tcgccgctgc tcgcatatct gcgctaggga
     1261 gcctctgcgg aacaatggcg atcgcgacgg tgggctgttc gggatgggat gcgcgaagcg
     1321 agccgaagcg aagggtcgtt ccccttcgcg aggctcgcgc caagagagac cgcccgaaca
     1381 gctaccgtcc cttcaggctg ctgcccaatg cggcgtctct ctcgacggtc tccttggctc
     1441 gtaggctcgg ctacgacctg catcgcccgt ctcgcatcca tccgcatttg gccgtgcagc
     1501 gcgatcgtca tagttctgca gaggctccct aagcattgaa cggcgtcagc ctgcccgctg
     1561 tctgacggat ctttcagcgc aaccccgacg gtcgtaatct ccggcgagtc ttatcgtttc
     1621 gccgatgtcc ccggccgccg tcacgcccat ccgtcgtcga acgacccagc gggtacgcga
     1681 gcgattggaa cgtcaaaccg aactcgattg gctgcgcgga ctgatgctgg tgctgatgac
     1741 gatcacgcac ctgccgacgt ggttcagctc gcacgtcggc cagccgttcg gattcgtatc
     1801 ggccgctgaa ggcttcgtct tcctgtccgc gtacctggtc ggctcggtct attcggggac
     1861 ggctctacgg cacggcttcg tcgcgatgcg caacgcgatc tggcgacgcg ccgcaaaggt
     1921 ttatgcggcg caggtggctc tgttgctgct cctgttctgg gtgctgctgc cgtatgcgac
     1981 atcgcagggc gcccacgcga tcaccgatct cgcgtcgttt tatgccgagc ggccgcacac
     2041 ggcgctggtg tcggcgctcg cactggcgta caacccgcca ttgctcgaca tcctgccgat
     2101 gtacgtgctg ttcatgctgg catcgccact gctgctcgag tacggctggc agcgcggatg
     2161 gggaccgctt atcgggctca gcgccgccat gtggctgctc tcgcagcacg atggtggacg
     2221 cgacatctac ggctatttcg ccggcattgt cggctggccg gtgccctaca accagacggg
     2281 cgcgttcgcg ttcctcgcat ggcagctgct atgggtcgcg ggattgcgcg ccggcgcttt
     2341 gcacgtgcag gaggcgcagg gcggagagcc ccgcacggcg tggtcgcggc ccgtgctgtg
     2401 gacggcgacg gcgctggctg tcggcttctt tgcgtggcgc cacatcaccg ggcaggttcc
     2461 gttcggcgat cacggctcgc tgaatgcgct cttcgacaag tggcatctcg ggccgatgcg
     2521 gcttttgaac ttcgccgtgc tggcgatcct ggcggtgcat ggccgccggt ggctcgtcgc
     2581 cgtggcgcag cgctcgtcga tcgcaacgct cggccgcgcg tcgttgacgg tgttcgcggc
     2641 gcacctggtc ctgtgcctga cgctgctcgc gtcgggcgag acggcgctcg attcgcagca
     2701 cgtcagcctg ctcgacggca cgctgctcgc cggaacgctg gtgacgcttt acgcgatcgc
     2761 gcgcgcgacg ctcggcggca gcaagttgat cgcgaggtgg actacggtcg cgccgccgaa
     2821 ggcgaccgcc gacgtcagtg cacgtgcggt ccgatgacgc gcttccacag cgcgtagccg
     2881 tcagcgttga ggtgcagcga atccgcgcgg aatagttctg cgcgcggctt gccggcgctg
     2941 tcgagcatcg gcgtgtagac gtcgatgaac gcaaccttgt cgaggccggc ggcgtagtcg
     3001 cggatcagcg cgttcgtttc cttgatctgc ggcatgaggc ggatacggag cgggcttggc
     3061 ttgatcgaga tataggcgat ccgcgtgtcc ggcagctcgt ggtgcacgcc gtcgacgaac
     3121 gccgcgaagc ggtgcagcac ttcggcgggg gtcgttcccg cggcgaggtc gttatcgccg
     3181 gcgtagacca ggacggtgct cggacggtag cggatgacga gccgattgag gttcttgacg
     3241 caatcggcga gctccgagcc gccaaagccg cggttgatga cgaccggcag gtccttgaac
     3301 tgttcctgca agtcgctcca gagccggatc gacgagctgc caacgaacag cacgccgccc
     3361 gcggggtgcg ggtgcgacgc atcgtcgtcg gcgaacgcgg caaatgcctg ctcccacggc
     3421 gagcctttcg gcgcgtcggc ggcaggggtc gacggcgcgc ccgccgcgtg ggctgcgaag
     3481 gtgagggcgc taaacagcgc gcaggcaaac gtcgaaaccg acttgaacgt catc
//
LOCUS       DASSVX010000085         4850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1285860, whole genome shotgun sequence.
ACCESSION   DASSVX010000085 DASSVX010000000
VERSION     DASSVX010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285860"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VFO53_03870"
     CDS             complement(<1..1430)
                     /locus_tag="VFO53_03870"
                     /inference="COORDINATES: protein motif:HMM:NF022073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HET8875445.1"
                     /translation="MHGRALKRLGAVLALAPLPFAASAAPGCASVEQFLVGKATGVAC
                     FHTDDLRTANTSTTNPVTPKDNSITTFADGTPLPGLASGTGSYTPTRDRGVISNGAAP
                     SSGPVPGIQVEGWFADDVTKQARFVLRFPDDWNGRLVVAGASGTRSEYNGDFAWSDYV
                     LPKGYAYASQNKGVLNFYIVSAASPTQPAPDTCRVNPPGGLLSQFWVHFYDVDPGKPF
                     TQWTQYMLQTAQLAQSAVKANYSKFPRRIYAVGTSNGGYQVRRAIEEAPNLFDGGVDW
                     EGTYVAPTENILVDLPVAVKNYPDYVASGYNASSVAAQNILAAGYPPDIVHTDATSGK
                     VTSLWGSYYNDFWEVTACQWQQRFDPTYQTYTAGLGNYDLLLRLGLPGVLDSITAVTT
                     TGKIKKPLITVAGTMDALLPIKHQARAYEDAVDASRKGNNDKRNAQYRLYEVQNGNHI
                     EAYVTPFPELVEIQPHAQKAFDLLVA"
     gene            1708..2262
                     /locus_tag="VFO53_03875"
     CDS             1708..2262
                     /locus_tag="VFO53_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HET8875446.1"
                     /translation="MILVRPFNALAAGVVTLCVAACSGVHFGMAHDTETDAKPDTTDY
                     GQVAPLRAIGGSAIFGKIRVIDRSSGANVLVSMMNVPPGDYRIAFHDTPNCSSPNGFS
                     AGPLWAPSGNDPRNLIPVQHVNSEDRVELSLRVPAVHAKGREGVAGHSVVVYAGPKVT
                     DARPGVPNERMACGVFEPAHQVAF"
     gene            2303..3088
                     /locus_tag="VFO53_03880"
     CDS             2303..3088
                     /locus_tag="VFO53_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8875447.1"
                     /translation="MGLLDDRKILITGVLSDRSIAYGVAKACEREGATLAFTYASEEL
                     KPRVVKLAAAFGACPVLACDVTSDEQIASLFDALGREWGGLDGLLHSIAFAPREALSG
                     DYLDAVTRSAFATAHDVSSYSLAALAKAARPLLAGRNASLVTLTYLGAVRALANYNVM
                     GLAKASLEANVRYLAAAMGPEGTRVNAISAGPIKTLAAAGIGGFSKILRFVEQTAPLR
                     RNVTIDEVGNVAAFYFSSMSSAITGEVTYADAGFSQVAAGLTE"
     gene            3088..4743
                     /locus_tag="VFO53_03885"
     CDS             3088..4743
                     /locus_tag="VFO53_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8875448.1"
                     /translation="MARAPRIKDGPYDAVIVGGGHNGLVCAAYLAGAGMSVCVLERRD
                     VVGGAAVTEEFHPGFRNSLASYTVSLLAPQVIRDLDLSAHGLAIVKRPLANFLPLPGD
                     AFLKVGGAVAATQAEVAKFSTRDAERLPAYYAMLSRVADVLRALLHVTPPNVGAHRGG
                     RVAMALDFWKTAKPFRALDRPGQRDVLDLFTKSAGEILDRWFESAPIKAAFGFDAVVG
                     NFQSPYAPGSAYVLLHHVFGEVNGEPGQWGHAIGGMGAITQAMAKACVARGVTIRTSA
                     PVRRVHVERGRATAVVLESGETIAARRVVANVNPKLLFQQLVAPEHLDDDFRARIDGY
                     RCASGTFRMNVALAALPDFTCLPGNGEARLHHGSGIIVAPSLAYMERAYFDAKTLGWS
                     RAPIVEMLIPSVLDASLAPPGMHVASLFCQHAHPRIGDVLPGHTWDDHRDEVADLMIA
                     TVNGVAPNFARSVLGRRVLSPLDLEREFGLTGGDIFHGALALDQLFSARPVLGNADYR
                     MPIAGLYLCGSGAHPGGGVTGIPGRNAAREILRDARKRIEAPR"
BASE COUNT          735 a         1593 c         1645 g          877 t
ORIGIN      
        1 tgcgcgacga gcaggtcgaa cgccttctgc gcgtgcggct ggatctcgac gagctcgggg
       61 aacggcgtca cgtacgcttc gatgtggttg ccattctgta cctcgtacag ccggtactgc
      121 gcgttgcgct tgtcgttgtt ccccttccgt gaggcgtcga ccgcgtcctc gtacgcccgc
      181 gcctgatgct tgatcggcaa aagcgcgtcc atcgttcccg caaccgttat cagcggcttc
      241 ttgatcttgc cggtggtcgt taccgccgtg atgctatcca gcacgccggg gagcccgaga
      301 cgcaagagca agtcgtagtt gccaagtcct gccgtgtagg tctgatacgt gggatcgaag
      361 cgctgctgcc actggcatgc ggtgacctcc cagaagtcgt tgtagtagct tccccacagc
      421 gacgtcacct tgccgctcgt cgcgtcggtg tgcacgatgt caggcggata gcctgcggcc
      481 aggatgttct gcgcggcgac gctgctggcg ttgtagcccg acgccacgta gtccggataa
      541 ttcttcacgg cgacgggcag gtcgacgagg atgttctctg ttggcgcgac gtacgtgcct
      601 tcccagtcga ccccgccgtc gaacaggttc ggtgcttcct cgattgcacg gcgcacctgg
      661 tatccgccgt tggacgtccc gacagcgtag atcctgcgcg ggaatttgct gtagttggcc
      721 ttcacggcgc tttgcgcaag ctgtgcggtt tgcagcatgt actgcgtcca ctgcgtgaag
      781 ggcttgccag gatcgacgtc gtagaagtgg acccagaatt gcgaaagcaa tccgcccggt
      841 gggttgacgc ggcaggtatc gggtgccggt tgcgtcggcg acgccgcact cacgatgtag
      901 aaattgagca cccccttgtt ctgcgacgcg taggcgtagc ccttcggcag aacgtaatcg
      961 ctccacgcga agtcgccgtt atattcgctg cgggtcccgg atgcgccggc gacgacgagc
     1021 ctgccgttcc agtcgtcggg gaagcgcaga acgaaacgtg cctgcttcgt gacgtcgtcg
     1081 gcgaaccacc cttcgacctg gatgcccggg actggtccgg acgaaggcgc agctccgttc
     1141 gatatcacgc cacggtccct ggtcggtgtg tacgacccgg tgccgctcgc caatcccggc
     1201 agtggcgtgc cgtcggcgaa cgtcgtgatc gaattgtcct tgggcgtaac cgggttggtg
     1261 gtcgacgtgt tcgccgtgcg cagatcgtcg gtgtggaagc atgccacgcc ggtcgccttg
     1321 ccgacgagaa attgctcgac tgacgcgcat ccgggggccg cgctggctgc aaacggcagt
     1381 ggcgcgagcg cgagtacggc gccgagccgc ttcaatgctc ttccgtgcat ggacaatctc
     1441 ctgttctcgt gatgagcgag cgtgggcgca atcgcattcg aaggctcgac cgtaaccacg
     1501 gcaacgcact gggttgtggc gcacatgaca tcggcgcacg ccgtgcgcga ttcgtgtgcg
     1561 gatcgcgatg ctaaccgcaa ttgaagagcg acgcgaggcg tcacttttca atcggacgaa
     1621 cggtacgcgc aggacgcggt tcgggcgcga ccgcggcgca tgcgacaatc gcggcagcgc
     1681 gtggcggatg cgcgcgggag aacgcgtttg atcctggtac gtccattcaa tgcactggcc
     1741 gcgggcgtgg tcacgctgtg cgttgccgcc tgctcggggg ttcatttcgg catggcgcac
     1801 gacaccgaga cggacgcgaa gcccgatacc accgactacg gacaggtcgc accgctgcgt
     1861 gccatcggcg gctcggcgat attcggcaag atccgggtga tcgaccgcag cagcggggcc
     1921 aacgtcctcg tgtcgatgat gaacgtgccg cccggcgatt accgcattgc gttccacgac
     1981 acaccgaact gctcgtcgcc gaacggcttt tcggcaggtc cgctgtgggc gccatcgggc
     2041 aacgatccgc gcaatctcat tcctgtccag cacgtcaaca gcgaagaccg ggtcgaactg
     2101 tcgctgcgcg tgccggccgt gcacgcgaaa ggccgcgaag gcgtcgccgg ccacagcgtg
     2161 gtcgtctacg ccggtccgaa ggtcaccgac gcgcgccccg gcgtgccgaa cgagcgcatg
     2221 gcctgcggcg tgttcgagcc ggcgcaccag gtagcgttct gagcgtattc ttttcgcgcg
     2281 cgctttcacc ccggcaattt cgatgggcct gctcgacgac cgcaagatcc tgatcaccgg
     2341 cgtgctgtcg gaccggtcga tcgcttatgg cgtggcgaag gcgtgcgaac gcgagggcgc
     2401 aacgctcgca ttcacgtatg cgagcgagga gctgaagccg cgcgtcgtca agcttgcggc
     2461 ggccttcggc gcgtgcccgg tgctcgcctg cgacgtcacg tccgacgagc agattgcgtc
     2521 gctgttcgat gcactgggcc gtgaatgggg cggcctcgac ggcttgctgc attcgatcgc
     2581 ctttgcgccg cgcgaggcgc tgtcgggcga ctacctcgac gccgtgacgc gctcggcgtt
     2641 cgccacggca catgacgtat cgagctacag ccttgccgcg ctcgccaagg ccgcgcggcc
     2701 gctcctcgcg ggccgcaatg cgtcgctcgt gacgctcacc tatctcggcg ccgtgcgcgc
     2761 gctcgccaac tacaacgtga tgggactcgc gaaggcgagc ctcgaagcga acgtgcgcta
     2821 tctcgccgcc gccatgggac ccgagggcac gcgcgtgaac gcgatctcgg cgggaccgat
     2881 caagacgctc gcggcggcgg gcatcggcgg gttctcgaag attctccgtt tcgtcgagca
     2941 gacggcgccg ttgcggcgca acgtcacgat cgacgaggtg ggcaacgtcg ccgcgttcta
     3001 cttctcgtcg atgtcgagcg cgatcacggg cgaggtcacg tacgccgacg ccggtttttc
     3061 gcaggtggcc gcgggactca ccgagtagtg gcgcgcgcgc cgcgaatcaa ggacgggccg
     3121 tacgacgcgg tgatcgtcgg cggagggcac aacgggctcg tgtgcgccgc gtatctcgcc
     3181 ggcgccggca tgtcggtttg cgtgctcgag cgccgcgacg tcgtcggcgg cgccgccgtg
     3241 accgaggagt tccatcccgg ctttcgcaac tcgctcgcga gctacacggt gagcctgctt
     3301 gcgccgcagg tcattcgcga tctcgatctt tcggcacacg ggcttgccat cgtcaagcgg
     3361 ccgctcgcca atttcctgcc gctgccgggc gatgcgtttc tcaaggtcgg cggcgctgtg
     3421 gcggcgacgc aggcagaagt cgccaagttc tccactcgcg acgccgagcg cctgccggcg
     3481 tattacgcga tgctttcgcg cgtggccgac gtgctgcgcg cgctgttgca cgtgacgccg
     3541 cccaacgtcg gtgcgcatcg cggcggccgc gtcgcgatgg cgctggactt ctggaagacg
     3601 gcgaagccgt ttcgcgcgct cgatcggccg ggccagcgcg acgtgctcga cctcttcacc
     3661 aaaagcgccg gcgaaatcct cgaccggtgg ttcgagtcgg cgccgatcaa ggccgccttc
     3721 ggcttcgacg ccgtcgtcgg caatttccag agcccgtacg cacccggttc ggcgtacgtg
     3781 ctgctgcatc acgtcttcgg cgaagtgaac ggcgagccgg ggcagtgggg ccatgcgatc
     3841 ggcgggatgg gtgcgatcac gcaggcgatg gcgaaggcct gcgtggcgcg tggcgtcacg
     3901 atccgcacgt cggcgccggt gcgccgcgtg catgtcgagc gcggccgtgc caccgccgtc
     3961 gtgctggaaa gtggcgagac gatcgcggcg cggcgcgtcg tcgcgaacgt gaatccgaag
     4021 ctcctgttcc agcaactggt ggcgcccgag catctcgacg acgatttccg cgcgcgcatc
     4081 gatggctacc gctgcgcttc cggaacgttc cggatgaacg tcgcccttgc ggcactcccc
     4141 gatttcacct gcctgccggg caacggggag gcgcggctgc accacggcag cggaatcatc
     4201 gtcgcaccgt cgctcgcata catggagcgc gcgtatttcg acgcgaagac gctcggctgg
     4261 tcgcgcgcgc cgatcgtcga gatgctgatc ccgtcggtgc tcgacgcgtc gctggcgccg
     4321 cccggcatgc atgtcgcgag cctcttctgc cagcacgcgc atccgcggat cggcgacgtg
     4381 ctgccgggtc acacgtggga cgatcaccgc gacgaggtcg ccgacctgat gatcgcgacg
     4441 gtgaacggcg ttgcgccgaa cttcgcgcgc agcgtgctcg gccgacgcgt cctgtcgccg
     4501 ctcgatctcg aacgcgagtt cggcttgacc ggcggcgaca tcttccatgg cgcgctcgcg
     4561 ctcgatcaac tcttctccgc gcggccggtg ctcggcaacg ccgactaccg gatgccgatc
     4621 gccggcttgt atctgtgcgg ctcgggcgcg catcccggcg gcggcgtcac cgggataccg
     4681 ggacgcaatg cggcgcggga gattctgcgc gatgcacgca aaaggatcga agccccccga
     4741 taagccgggt tctgttacgg cgcgcgagcg ctatgaccgt cattcctcta ggcccgccgt
     4801 tgccgacggg ctcgagccgc ctacccgcgg actcggcgag cagcgtcgtc
//
LOCUS       DASSVX010000086        19613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1086009, whole genome shotgun sequence.
ACCESSION   DASSVX010000086 DASSVX010000000
VERSION     DASSVX010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 19613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19613
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086009"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VFO53_03890"
     CDS             <1..842
                     /locus_tag="VFO53_03890"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453714.1"
                     /note="catalyzes the ATP-dependent formation of a
                     phosphodiester at the site of a single strand break in
                     duplex DNA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET8875449.1"
                     /translation="LVTERFPELQALGDALPDGTVLDGEIVALDDGDIAPFALLSTRI
                     GRKRLSPKFLAAAPVAYVAYDILEAGGADLRELSQLARRARLAEVAARLDAAPLRLSP
                     LVERATWDGYAKLRTQSRSRGAEGLMLKRRDAPYGIGRTGNAWWKWKVDPMTVDAVLV
                     YAQRGHGRHASLYTDYTLAVWDTRGGTRALTPFAKAHSELSAEETGAVDRIVRKTTIE
                     KFGPVRGVTPTLVFELAFEGIKRSPRHKSGIAVRSPRIVRWRTDKGIDEADTIEALEA
                     LLR"
     gene            complement(868..1518)
                     /locus_tag="VFO53_03895"
     CDS             complement(868..1518)
                     /locus_tag="VFO53_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875450.1"
                     /translation="MKSACIALIALALTACASLGPAAPGIDGKPLGEFVDDLKTQLRA
                     VHWRIRSTSAACGSAEPREIDLRNAQVALDLERIGTASVDGQIRLVALPLAGLAVAPF
                     ASGSSERKYTQELALKFDVTGPSRLYDAGEAAGAGGAIAQAVNAAIDGFVRSSADEPC
                     VRLTSLKLSLVIDVARDAAGGFKLVVPAAQLGAGASRRDVNTLTLTWDKVLSNALR"
     gene            complement(1515..1832)
                     /locus_tag="VFO53_03900"
     CDS             complement(1515..1832)
                     /locus_tag="VFO53_03900"
                     /inference="COORDINATES: protein motif:HMM:NF042532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protealysin inhibitor emfourin"
                     /protein_id="HET8875451.1"
                     /translation="MRIDFRADGGLAAFPGLSAPVTIHCDALPPSENARVRELVQRAD
                     FFALPSADRQPAAPDARSYTIAIDDGSRCRTVTVREPIADPALRDLVDTLRDKARDAR
                     RTP"
     gene            complement(1841..2902)
                     /locus_tag="VFO53_03905"
     CDS             complement(1841..2902)
                     /locus_tag="VFO53_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HET8875452.1"
                     /translation="MRRQSHCACRILPPALLQRIARNGNALQREAALDTLAVDHTLRL
                     GRATWQLLDAGTRHGLFGISGAGKQRSIYDAHGAEALPGRLVRAEGAPAQGDAEVNEA
                     YDGLGATFDFFADVYDRNSIDDAGLHLDASVHYGRRYDNAFWNGQQMVFGDGDGDLFN
                     RFTISLDVIAHELTHGVTGDEARLVYLGQAGALNESVSDVFGSLVKQYARRERAADAD
                     WLIGAGLFTAKVHGVALRSMKAPGTAYDDPVLGRDPQPATMDGYVHTQADDGGVHTNS
                     GIPNHAFYLAAIALGGYAWEKAGRIWYETLRDKRMKPTATFRQFARLTAQNAAHFFGS
                     AERQAVIAAWAEVGIKATG"
     gene            complement(3112..3294)
                     /locus_tag="VFO53_03910"
     CDS             complement(3112..3294)
                     /locus_tag="VFO53_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875453.1"
                     /translation="MKSILDPTFRYTSSANTDLRKTFARIRRESRRQAQGSAGREEGD
                     AVKKVTPIFRGQRPAA"
     gene            complement(3512..4111)
                     /locus_tag="VFO53_03915"
     CDS             complement(3512..4111)
                     /locus_tag="VFO53_03915"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET8875454.1"
                     /translation="MPPVTTALIVANIAVYLLQTAVPQIVVPFALWPLATSSMNMGAS
                     FAPWQLVTYAFLHGSVLHIAFNMFALYMFGSAIEQVFGTRRYLLFYFVSVISAAITQL
                     VVAMLAHAIYPTIGASGGVFGLLLAYAMYFPRNRVMLLFPPIPMPARVFVVLYAALEL
                     ILGVSGSQEGVAHFAHLGGMIGGYLLLRYWRGGIRRSSR"
     gene            4248..4868
                     /locus_tag="VFO53_03920"
     CDS             4248..4868
                     /locus_tag="VFO53_03920"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET8875455.1"
                     /translation="MQSDAAPPAPRVVRHPVVMIDDFLPHAAWQPLLARVLASEAQFA
                     ASGTHDRRADYRQSLVLNPPPDLVHPVVTRVRAAMPRVVPALRIPPIVVGVVEAQVTA
                     SVDGSFFGVHTDAGNDKVRKRHMTYVYYFNRQPRGFSGGELRVYDDIVRNGKLARAET
                     FSVIEPRHNRIVFFWAKTMHEVMPVSVPSRTFADARFTVNGWINAA"
     gene            complement(4892..5560)
                     /locus_tag="VFO53_03925"
     CDS             complement(4892..5560)
                     /locus_tag="VFO53_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875456.1"
                     /translation="MCSQRLPRPGRLCRECDRELERARYAGVSIGELAPALPSADASR
                     MAVSGWRARVRSPGPVIAAAFFVGLVAAIALHVVDKSEAAVTHGSVMLDARASRDLRR
                     VSIGDPSPLPVGESDRAPASPPAAAPAKIATPVRPPAPRDPVIAPHDVSGATPQIALA
                     APAPREADVVAEPAGMLRDALARCGGERFLARPACEERARARYCGDAAASAQCATPER
                     DHGQ"
     gene            5836..8628
                     /locus_tag="VFO53_03930"
     CDS             5836..8628
                     /locus_tag="VFO53_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET8875457.1"
                     /translation="MRLIWPFVAIVVLLLALGMASLSVMSGVRAYISAQSAWSNAQKA
                     AVESLEQYAQSHNEDYFDRYVEESDVLAGARNARIELDKPFPDFTAARRSLRRAGSHP
                     ADIGGMVNLFRRFRWFGFMAEAVRLWTVSDALHEQIDANAKAMHAMVRGGHAATPDLQ
                     PALIAIRGIDRELTMVERAFSATFAEASRRVELVLTIATLIVALALVAAAALRTQRLV
                     RKEDAFHGALRASQQRFDYAISGTDDGIFDWRLAHHELYLSPRFEELLGHAPGSLHET
                     PASFLRRVHGDERALALAQVKRYLASGQPFSLELRLRMADGGSRWFRMRGRSVLGARG
                     KPERMAGSLTDISDRKRAEAETLAQKERAQVTLASIADAVITVDTSGNVEYMNPVAER
                     LTGFAHGEALGIALPGVFAVQDDATGVEVPDPVARAFRDGATIQSEGNVVLRRRHDGP
                     IAIDYAAAPIRDDAHNISGVVLVFHDMSRERQYATRLAHLASHDALTGLLNRREFERR
                     VTTALIESQYEANNHAVLYVDLDEFKVVNDTCGHAAGDELLRQVSALLRPRLREGDTL
                     ARLGGDEFGVLLEHCGPGPAFAIADALRTTIGDFHFQWNQRSFKIGASVGVVNVGGGP
                     QTLAGVLSAADAACYMAKDKGRNRVQVYSPESDEVTLRRGEMEWVNRIHQALAEDRFC
                     LHAQPVQGTAANDDSSPYTELLLRLRDDRGELVPPTSFIPAAERYHLMPAIDRWVISS
                     AFAILSRQFEGGPAPAGIFAINVSGASIGDDEFLDFVQAQFVRFAIPHTSVCFEITET
                     TAVASLSKATQFMTTLRTLGCRFALDDFGVGVSSFTYLKHLPVDYLKIDGSFVKDMLR
                     DPVNHAMVEAIHRIGHIMGKMTIAESVEHRDTLKALRAIGVDYAQGFGIARPAPFGRM
                     RAVRTGPQKRQAAGGA"
     gene            8846..9592
                     /locus_tag="VFO53_03935"
     CDS             8846..9592
                     /locus_tag="VFO53_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET8875458.1"
                     /translation="MANTVSTREGHRVPDVSFRIRRNGDWATLTTQDIFAGKDVVVFS
                     LPGAFTPTCSSTHLPRYNELAPVFKAQGIDTIVCVSVNDPFVMEEWGKDQEAGNVVLL
                     PDGNGEFTGKMGMLVDKTDLNFGKRSWRYSMLVRDGTIEKMFVEPQEPGDPFKVSDAD
                     TMLRYINPNAKKPDQVAILTRDGCSFCAKAKERLADAGYDYVEVPLPHTIRTKAVGAI
                     AGAQTVPQVFVNGRLIGGTNELDAFLRKAA"
     gene            9589..11004
                     /locus_tag="VFO53_03940"
     CDS             9589..11004
                     /locus_tag="VFO53_03940"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET8875459.1"
                     /translation="MTTFSTDVAVIGAGTAGLAAYRAAKAAGRTALLIEGGTYGTTCA
                     RVGCMPSKLLIAPAEAAHALARFDAFGLRLPGEVVVDGEAVMARVRAERDRFVGFVLQ
                     GVAAIPPADRMDACARFVDDTTLVAADATITAGAVVIATGSSPAYPAAWTALGERLVT
                     NETIFDWTRLPESVVVFGPGVIGLELGQALHRLGVRVKVFGRGGGVGPLSDPELLAEA
                     RRLFAAEFYLDTDARAEVTRDGDRVVVRYRPPDGELRTERFDYLLAATGRTPNVRTLR
                     LEATTLALDAQGVPLFDRETLQCGSAPIFIAGDANGDVPLLHEAADEGRIAGENAARF
                     PDVHAGLRRAGLAIVFTDPQIAVIGGGLRAAREAAAGFVAGTVSFEDQGRARVMQRNR
                     GRLHVYAEIGSGLLLGAEMIGPDAEHLAHLIAWARQLDLTIGEMLTLPFYHPVVEEGL
                     RTALRDAYAKLDAASNERRAA"
     gene            complement(11020..11166)
                     /locus_tag="VFO53_03945"
     CDS             complement(11020..11166)
                     /locus_tag="VFO53_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875460.1"
                     /translation="MKANRNGRSPGTVGVYDRPHPLRTRKVLVPAVIFVLIAIVYALY
                     FLLR"
     gene            complement(11239..12252)
                     /locus_tag="VFO53_03950"
     CDS             complement(11239..12252)
                     /locus_tag="VFO53_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875461.1"
                     /translation="MKTIVGSFDSFDEANRVARELMDDGFRDSDVSVVASNVRNDYRS
                     GTGTSSGAGVSAPADTSTGVAGTESMLGARGTRTSTGFTNDTGAGTMAGMGTASGGTA
                     ARGTTSSEHYTRSAADNAVSEDGAGLTDAAIDRHRGVDDRVDDGSNAAAGAVTGGVVG
                     GAAGLAASLMGLAIPGIGPIIAAGPIVSLLTGAGVGAVAGGLIGGLTDMGVSRPDAEY
                     YAEAVRRGGALVTVRADDARADRAAEIMRSHGAIDIERRAEQWRERGWTGFDEKAQPY
                     TPADLDRDRDLYGRRDRETGIASGASIGGVLTGGPAGSIPDRDTAGSAGDRTTPRTPG
                     AGT"
     gene            12508..13482
                     /locus_tag="VFO53_03955"
     CDS             12508..13482
                     /locus_tag="VFO53_03955"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET8875462.1"
                     /translation="MFSILIRWLCAAAAATLALAGVASAQDYPARPIRVIVPFSPGGA
                     VDGPMRLIAQELGKRLGQPVVVENKPGAGATIGSDIVAKSPADGYTLLLASQTNAISA
                     TLYAKLPFDPIGDFTPVTLIGREPGVVVVNPAVPAKTLHEFIAYVKQHPGEVNYASSG
                     NGSGQHLFAALLASRTGMKMNHVPYRGSGQATTDLLSGVVAMAIPGTAGMVGHIKAGK
                     LRALAVTGAGRSPQLPDVPTVAESGVQGYEAYVWMGLLAPKGTPPSIIERLNRDVVAV
                     LGEDQVKRYMANAGIEIVGSTSADFGRFFRAERDQWAKVIHDTGAHIE"
     gene            13497..14435
                     /locus_tag="VFO53_03960"
     CDS             13497..14435
                     /locus_tag="VFO53_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8875463.1"
                     /translation="MSESRQRTSTIDAPVEGVAHDTHKGDVRLFLWEKFVGEPSGKPV
                     VLFVHGSSMASQPTFDLTVPGRPDSSVMDWFARRGFACWSVDMEGYGRSDKRRDIACD
                     IANGADDLAAATNYMRDARGIESVMAYGISSGALRAALFAERHPRRVARLALDAFVWT
                     GEGAPTLEQRRSKLPEFRARNRRPIDRAFVESIFARDHPGCAEPGVVAAFADAILALD
                     DSMPNGTYVDMCSKLPVVDPAKIAMPTLIMRGQYDGIAALDDLIEFFRRLPNADKQFA
                     MMPGISHASFQQKNYRVAYHILHAFFTQPAPVYGGE"
     gene            14454..15185
                     /locus_tag="VFO53_03965"
     CDS             14454..15185
                     /locus_tag="VFO53_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HET8875464.1"
                     /translation="MAVQPTLLLIADIAGYTRFMKMHDTSLVHAQQIIADLLEAVIDA
                     AGPSLRLAKLEGDAAFFYMPSPADGAPAPALIEERTAAIYRAFHARAADIKANTLCPC
                     DGCQQAGNLKIKLVGHAGGVAVQKVKRLTELAGVDVIVVHRMLKNDVPVSEYLLVTKP
                     VHAMLGDALRERAAAFDLELDDLGKTSAWYIDLADCVAPLPPARKRSPLARVGGLLRF
                     EVRNLPYALHLRKARVGFRNLPDAH"
     gene            15258..17117
                     /locus_tag="VFO53_03970"
     CDS             15258..17117
                     /locus_tag="VFO53_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003548396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875465.1"
                     /translation="MSIEFQTRGLTIRALDRVSFRIAPGTTLALVGESGSGKSITAQA
                     MMRILPPSASITSGQIVFTQPDGRERPVDIVRLAPDGRKIRRIRGRHISMIFQEPMVS
                     LSPLHTIGDQVSEALFLHHRIGHREGMARTEDMLRRVGFPDPHRALRTYPFQLSGGLR
                     QRAMIAMALVCRPSLLIADEPTTALDVTIQAQILALVKELQAELGMAVLIITHDLGVV
                     ANVAEHVVVLYQGRVMEQGRVEAIFERPAHPYLRALLRAVPHFDMKPGERLVSVRSVD
                     YQTGPLVETREAWPAGADAPHLTVQSVVKTFAQGTSGFLVRREGPAFRAVDDVSLAIR
                     RGQCFGLVGESGCGKTTLSKVIMRAMEPDSGKVLYNDRGTTIDVTRASGDTLMQFRRR
                     VQYIFQDPFGSLSPRMTVFDILREPLVIHGIGDADYRAEMAKELMRLVGLDPRYLSRY
                     PHSFSGGQRQRIGIARALALSPDLILCDEPVSALDVSIQAQILNLLKDLQAELGLTYL
                     FISHNLAVVDYVADEIAVMCRGRIVELAPREVLFRDPMHPYTLGLLAAVPYPDLRRKL
                     DLAAVQAGAARSPDEWPAPFAWADANAGELVDVGDGHRVRMQTAAAAREPVTT"
     gene            17277..19187
                     /locus_tag="VFO53_03975"
     CDS             17277..19187
                     /locus_tag="VFO53_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020286577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8875466.1"
                     /translation="MLNNAMAAARLLLVALVVLFAAPALAEEPPSLAADVAAGKLPPL
                     AERLPRAPFVVDVARAGWSPGRYGGELRMLMAKDRDIRMMVVYGYARLVGYDEHLHLV
                     PDILQSLDNDGNRVFTLHLRPGHKWSDGQPFTADDFRYFWEDVANNKDLSPFGLPRAL
                     LVDNHGPKFEVLDATTVRYTWDEPNPQFLTALAGPSPLFIYRPAHYLKQFHARYAGLD
                     KANARAVAAGSRNWAGLHQKMDEQFRFDNPDLPTLEPWINTTGLPTTRFVLVRNPYFH
                     RVDRMGHQLPYIDRVIVGVTDDKLIPARTGAGNVDLQARYLRFDNYTFLKQAETRNRY
                     RVYLWEKAIGSQVALYPNLNVDDPAWRTLMRDVRFRRALSLGIDRHEINEVVYFGLGQ
                     ESANTVLPRSALYRPEFRAAWAAFDPKRANALLDAIGLAKRDANGIRLLPDGRPMELI
                     IDTSGESTEETDVLELIRDTWRQLGIAMFARPSQREVFRKRVFSGKSMMSVWSGLNNG
                     VPTPEMSPSELAPTAQEQLQWPMWGQYYEQNRNGGEKPALPEVQELGRLYDEWRRATN
                     DDERERIWLRMLAIHADQVYTIGTVTHALQPVVVSDDLRNVPSEGIYSWDPGAYFGMY
                     HTDAFWFGTEAR"
     gene            19187..>19613
                     /locus_tag="VFO53_03980"
     CDS             19187..>19613
                     /locus_tag="VFO53_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875467.1"
                     /translation="MLQYITKRILMMIPTLFAISIIVFTIIQLPPGNYLESYIAELEA
                     QGESVDQQKVAFLREYYGLDKPLPEQYLHWAGGMLKGDFGYSFEFNMPVTKVIGDRML
                     LTVIVSFVTILFTWAVAFPIGIYSATHQYSWGDYGLSFLG"
BASE COUNT         3165 a         6740 c         6588 g         3120 t
ORIGIN      
        1 agctcgtcac cgagcgcttt cccgaattgc aggcgctcgg cgacgcgctg ccggacggca
       61 cggtcctcga cggagaaatc gtcgcgctcg acgacggcga catcgcgccg tttgcgctgc
      121 tctcgacacg aattggccgc aagcggctgt cgccgaagtt tctcgccgca gcaccggtgg
      181 cctacgtcgc gtacgacatc ctcgaggccg gcggcgccga tctgcgcgaa ctttcccagc
      241 tcgcgcgtcg cgcgcgtctc gccgaggtcg cggcgcggct cgacgccgcg ccgctgcggc
      301 tttcgccgtt ggtcgaacgc gcgacctggg atggctacgc aaagctgcgc acccaatcgc
      361 gctcgcgcgg cgccgaaggc ctcatgctga agcgacgcga cgccccgtac ggcatcgggc
      421 gcaccggaaa cgcctggtgg aaatggaagg tcgatccgat gacggtcgac gccgtcctcg
      481 tctatgcgca acgcggtcac gggcggcacg cgagtcttta caccgactac acgttagcgg
      541 tgtgggacac gcgtggcggc acgcgtgcgc tcacgccatt cgccaaggcc cattcggaat
      601 tgagcgccga ggaaaccggg gccgtcgacc gcatcgtgcg caagacgacg atcgagaagt
      661 tcggcccggt gcgcggcgta accccgacgc tcgtattcga gcttgcgttc gagggcatca
      721 agcgctcgcc gcggcacaag tcgggcatcg cggtccgcag tccgcgcatc gtgcgctggc
      781 gtaccgacaa gggcatcgac gaggccgata cgatcgaggc gctggaggcg ctgctccgct
      841 gagcgtgtcc cggagactct cgcacgctta ccgcagcgcg ttcgaaagca ccttgtccca
      901 ggtcagcgtc agcgtgttga cgtcgcgccg cgatgcgcca gcaccgagct gcgccgccgg
      961 gacgacgagc ttgaatccgc ccgcggcgtc gcgcgcaacg tcgatgacga gcgacagctt
     1021 gagcgaggtg aggcgcacgc agggctcgtc ggcgctcgag cgcacgaagc cgtcgatcgc
     1081 cgcattcacc gcttgcgcta tcgcgccgcc cgcgcccgcc gcctcgccgg cgtcgtagag
     1141 acgtgacgga ccggtcacgt cgaatttgag cgccagctcc tgcgtgtatt tgcgctcgct
     1201 cgacccggac gcgaacggcg cgacagcgag cccggcgagc ggcagcgcga cgagccggat
     1261 ctgcccgtcg acgctcgccg tgccgatgcg ctcgaggtcg agtgcgacct gcgcgttgcg
     1321 aaggtcgatc tcgcgcggct cggcgctgcc gcatgcggca ctcgtgctgc ggattcgcca
     1381 gtgcacggcg cgcaattgcg tcttgagatc gtcgacgaac tcgccgagcg gcttgccgtc
     1441 gatgcccggg gccgcgggcc cgaggctcgc acacgcggtc agcgcgagcg cgatgagcgc
     1501 aatgcaggca gatttcatgg tgtccgccgc gcgtcgcgtg ccttgtcgcg cagcgtatcg
     1561 acgaggtcgc gcagcgcggg gtcggcgatc ggctcgcgta ccgtgaccgt ccggcagcgc
     1621 gatccgtcgt cgatcgcaat cgtgtagctg cgtgcgtcgg gtgcggccgg ctgccgatcg
     1681 gcggacggca acgcaaagaa atcggcgcgc tgaacgagct cgcgcacgcg cgcattctcc
     1741 gacggcggca acgcatcgca atgaatcgtg accggcgccg aaagcccggg gaacgcggca
     1801 agcccgccat cggcgcgaaa gtcgatgcgc attcggcgcg ctagccggtg gccttgatgc
     1861 ccacctcggc ccacgcggca atgaccgcct ggcgttcggc gcttccgaag aaatgtgcgg
     1921 cgttctgcgc ggtgagccgg gcgaactggc gaaacgtcgc cgtcggcttc atccgcttgt
     1981 cgcgcagcgt ctcgtaccag atccggcccg ccttctccca cgcgtagccg ccaagcgcaa
     2041 tggcggcgag atagaacgcg tgattcggaa tgccggaatt cgtgtgcacg ccgccatcgt
     2101 cggcctgcgt gtgcacgtag ccgtccatcg tggcaggctg cgggtcgcgg ccaagcaccg
     2161 ggtcgtcata cgcggttccc ggcgccttca tcgaccgcaa cgcgacccca tgcaccttcg
     2221 ccgtgaagag gccggccccg atcagccagt cggcatcggc ggcgcgctcg cgccgtgcgt
     2281 attgcttcac cagcgacccg aacacgtccg aaaccgactc gttcagcgcg ccggcctgtc
     2341 cgagatagac gaggcgagcc tcgtcgcccg tgacgccgtg cgtgagctcg tgggcgatca
     2401 cgtcgagcga gatcgtgaag cggttgaaca gatcgccgtc gccgtcgccg aataccatct
     2461 gctggccgtt ccagaaggcg ttgtcgtaac ggcggccgta gtgcacgctc gcgtcgaggt
     2521 gcaggcccgc gtcgtcgatg gaattgcggt cgtagacgtc ggcgaagaaa tcgaatgtgg
     2581 cgccgaggcc gtcatacgcc tcgttcactt cggcatcgcc ctgggcaggc gcgccttcgg
     2641 cgcgcaccag ccggcccgga agcgcttcgg cgccgtgcgc gtcgtagatg ctgcgctgct
     2701 tgcccgcgcc gctgatgccg aaaagaccat gccgcgtgcc ggcgtcgagc aattgccacg
     2761 tcgcccgccc gaggcgcagc gtgtggtcga cggccaaggt gtcgagcgcc gcctcgcgct
     2821 gcagcgcgtt gccgttacgc gctatgcgct gcagcagtgc cggcggcagg atgcggcaag
     2881 cgcagtggga ctggcgacgc atccgatttt ctcccgagcg agcgagccgc cacgcggcgg
     2941 cggggccgtt ttaccacgca acggtcggtt cgtgaaccgg cccgggcgag cacgcgccgc
     3001 gtgcgacgcc gcgtcgaggc ggcatgcacc atgcgtgaac cggctaaacg tcgatcggca
     3061 attttttccg gcggcgacgc cgcgattgcc aacgacgcgg gggcggcgcg ctcaggccgc
     3121 cgggcgctgc ccgcgaaaga tcggggttac cttcttgacc gcatcgcctt cttcgcggcc
     3181 ggcggagcct tgcgcctggc ggcgcgactc acgccggatg cgcgcaaacg tcttgcgaag
     3241 atcggtattg gcactggatg tgtaacggaa ggtagggtcg aggatcgatt tcattgcgca
     3301 cctccatgcg cgaagcagcg acggttgcag cggccggcat ccgacggacg gccatacgat
     3361 gctgcaaccg acaacgacga acgaggttgc aagatttctg ccaacttcgg cgcggcttcg
     3421 ccggccccga tgcagcccgg acagccggcg gaaaacgcgc cgcctcgact gtcaagggaa
     3481 ccgacacttt gctcgccaaa ccgcgcgcat gtcaacgcga cgaacggcgg atgccgccgc
     3541 gccagtatcg caacagcagg tagccgccga tcattccgcc gaggtgcgca aaatgcgcga
     3601 cgccttcctg cgaccccgac acgccgagaa tcagctcgag cgcggcgtag agcacgacga
     3661 agacgcgcgc gggcatcggg atcggcggga acaatagcat cacgcggttg cgcggaaagt
     3721 acatggcgta ggcgagcagc aatccgaaca cgccgcccga tgcgccgatc gtcggataga
     3781 tcgcatgcgc gagcatcgcg acgacgagct gcgtgatcgc ggccgatatc accgaaacga
     3841 agtagaagag cagatagcgt cgcgtgccga agacctgctc gattgcgctg ccgaacatgt
     3901 acagcgcgaa catgttgaag gcgatgtgca gcacgctccc gtgcaggaag gcgtaggtga
     3961 cgagttgcca cggggcgaac gacgcgccca tgttcatgct cgacgtcgcg agcggccaca
     4021 gtgcgaaggg cacgacgatc tgcggcaccg cggtctgcag cagatacacg gcgatgttgg
     4081 cgacgatcag cgcggtggtg acaggcggca tcggagatcc ggttgagtgg agggagcgcg
     4141 gccgaggccg gcgtcgcaaa ggtgggggca ccggcccgat cgggcaagcg gctggaatag
     4201 aatgccatgc ttccgcggcc cgcccgcggc aacccgcccg cttcgccgtg caatccgacg
     4261 ccgctccgcc cgcaccccgc gtcgtccgcc atcccgtggt gatgatcgac gatttcctgc
     4321 cgcacgcagc gtggcagccg ctgctcgcgc gcgtgctggc gagcgaagcg caattcgcgg
     4381 catcgggtac gcacgatcgg cgcgccgatt accggcaatc gctggtgctg aatccgccgc
     4441 cggatctcgt gcatcccgtg gtcacgcgcg tgcgcgccgc gatgccgcgg gtcgtgcccg
     4501 cgttgcgcat tccgccgatc gtcgtcggcg tcgtcgaggc gcaggtgacg gcgagcgtcg
     4561 acggcagctt cttcggcgtg cacaccgatg ccggcaacga caaggtccgc aagcgccaca
     4621 tgacgtacgt ctactacttc aaccgccaac cccgcgggtt ctcaggcggc gagctgcgcg
     4681 tgtacgacga catcgtgcgc aacggcaagc tcgcgcgcgc cgaaacgttc agcgtgatcg
     4741 agccacggca caaccggatc gtgttcttct gggcgaagac catgcacgag gtgatgcccg
     4801 tgagcgtgcc ttcgcgcacg ttcgccgacg cccgattcac cgtcaacggc tggatcaacg
     4861 cggcctgacg ctgcctagcg cgtcacgcgc gctactggcc gtggtcgcgc tccggcgtcg
     4921 cgcattgcgc cgatgccgcc gcgtcgccgc agtagcgtgc gcgtgcgcgc tcttcgcatg
     4981 cggggcgggc gaggaagcgc tcgccgccgc atcgcgcgag ggcgtcgcgc agcatgccgg
     5041 ccggctcggc gacgacatct gcctctcgcg gtgccggcgc agcgagcgcg atctgcggcg
     5101 tcgcgccgga aacgtcatgc ggtgcgatca ccggatcacg cggcgcgggt gggcgcactg
     5161 gtgtcgcgat tttcgcgggt gcggccgccg gcgggctcgc cggcgcgcgg tcgctttcgc
     5221 cgaccggcaa cggtgaaggg tcaccgatcg acacgcggcg caaatcgcgc gacgcgcgcg
     5281 catcgagcat caccgaaccg tgcgtgaccg cggcctccga cttgtcgacg acatgcaatg
     5341 caattgcggc gacgaggccc acgaaaaacg ctgcagcgat gacgggcccc ggcgagcgca
     5401 ctcgcgcacg ccatccgctg accgccattc gcgatgcatc ggccgatggc aatgcgggcg
     5461 cgagctcgcc gatcgacacg ccggcgtaac gcgcacgctc gagctcgcga tcgcattcgc
     5521 ggcacagcct gcccggacgg ggtaggcgct ggctgcacat ccggcattgc atcatggccg
     5581 gcgcccgctc tcgacggaag gcatggacat ctcgtgcgca aaagaccgtt gcatgacggg
     5641 tcgctcgatg cgatctcggg tgcgatgacg ccgacaaagc aattcacggg cctgcagttg
     5701 ccggagcagc cgctgttggc ccaccgcttg ctttgaccgg ctggcgcagg gtcgcatgca
     5761 atgcgcgacc cgacgccgca gcctgtcatg cacaacccgg cgacaacaac tctccaacgc
     5821 acacgctttc gcgtggtgcg actcatctgg ccgttcgtcg cgatcgtcgt cctgctgctc
     5881 gcgctcggca tggcgagcct gtcggtcatg agcggcgtgc gcgcgtacat cagcgcgcag
     5941 agcgcgtggt cgaatgcgca gaaggcggcg gtcgaatcgc tcgagcaata cgcgcagtcg
     6001 cataacgagg actacttcga tcgttacgtc gaggaatcgg acgtcctcgc gggcgcacgc
     6061 aacgcgcgca tcgagctcga caagccgttt cccgatttca ccgcggcgcg tcgaagcctg
     6121 cgtcgcgcgg gcagtcaccc ggccgatatc ggcggcatgg tgaatctgtt tcgtcgcttc
     6181 cgctggttcg gcttcatggc cgaggcggta cggttgtgga cggtgtcgga cgcgctgcac
     6241 gagcagatcg acgccaatgc gaaggcgatg catgccatgg tgcgaggtgg ccatgccgcg
     6301 acgccggacc tgcaacccgc gctcatcgcg attcgcggca tcgaccgcga gctgaccatg
     6361 gtcgagcgcg cgttctcggc aacgttcgcg gaggcgtcgc gtcgtgtcga gctggtgctg
     6421 accatcgcca cgctgatcgt tgcgctggcg ctcgtcgcgg cggccgccct gcgcacgcag
     6481 cggctcgtgc gcaaggagga tgcgttccac ggcgcgctgc gggcgagcca gcagcgcttc
     6541 gattacgcga tcagcggcac cgacgacggc atcttcgact ggcgcctcgc gcatcacgag
     6601 ctctatctgt cgccgcgctt cgaggaactg ctcggccatg cgccgggctc gctgcacgag
     6661 acgccggcgt cgttcctgcg gcgggtgcac ggcgacgaac gcgctctcgc gcttgcgcaa
     6721 gtgaagcgat atcttgcgag cggacagccg ttctcgctcg agttgcggtt gcgcatggcc
     6781 gacggcggct cgcgctggtt ccgcatgcgc ggccgctcgg ttctcggggc gcgcggcaag
     6841 cccgagcgca tggcgggttc gctcaccgac atctcggatc gcaagcgcgc ggaggcggaa
     6901 acgctcgcgc agaaggagcg cgcgcaggtg acgctcgcct ccatcgccga tgccgtcatt
     6961 accgtcgaca cgtcgggcaa cgtcgagtac atgaatcccg tcgccgagcg tctcaccggc
     7021 tttgcccacg gcgaagcgct cggcattgcg ctccccggcg tgttcgcggt ccaggacgac
     7081 gccaccggcg tcgaggtgcc cgacccggtg gcgcgcgcgt ttcgcgacgg cgcgacgatc
     7141 cagtcggaag gcaacgtcgt gctgcgccgg cgccacgatg gcccgatcgc gatcgattac
     7201 gcggccgcgc cgatacgcga cgacgcgcac aacatcagcg gcgtcgtgct cgtgttccac
     7261 gacatgagcc gcgagcgtca atacgcgacg cggcttgcgc acctcgcaag ccacgacgcg
     7321 ctgaccggcc tcctcaaccg ccgcgagttc gagcgccgcg tcacgacggc gctgatcgag
     7381 agccagtacg aggcaaacaa ccatgccgtg ctctatgtcg acctcgacga gttcaaggtg
     7441 gtcaacgaca cctgcggcca tgcggcaggc gacgaattgc tgcgccaggt gagcgcgctg
     7501 ctgcggccgc ggctgcgcga gggcgacacg ctcgcccgtc ttggcggcga cgaattcggc
     7561 gttcttctcg agcactgcgg gccgggcccg gcgttcgcga tcgccgacgc gctgcgcacg
     7621 acgatcggcg acttccattt ccagtggaac cagcgctcgt tcaagatcgg cgcgagcgtc
     7681 ggggtcgtca acgtcggcgg cggcccgcag acgctcgccg gcgtgctctc ggcagccgat
     7741 gccgcgtgct acatggcaaa ggacaaggga cgcaaccgcg tgcaggtcta cagccccgaa
     7801 agcgacgagg tgacgctgcg caggggcgag atggaatggg tgaaccggat tcaccaggcg
     7861 ctcgccgagg accgcttctg cctgcacgcg caacccgtgc agggaaccgc ggccaacgac
     7921 gattcgtcgc cgtacaccga gcttctgctg cggctgcgcg acgaccgcgg cgagctcgtg
     7981 ccgccgacgt cgttcattcc cgcggcggag cgctatcacc tgatgcccgc gatcgaccgc
     8041 tgggtgatca gctccgcgtt cgcaatcctg tcgcgccagt tcgagggcgg gcccgcgccc
     8101 gcgggcatct ttgcgatcaa cgtctccggc gcgtcgatcg gcgacgacga gttcctcgat
     8161 ttcgtgcagg cgcagttcgt ccgcttcgcc atcccgcaca cgagcgtgtg cttcgagatc
     8221 accgagacga ccgccgtcgc gtcgctgtcg aaggcgacgc agttcatgac cacattgcgc
     8281 acgctcggct gccgcttcgc gctcgacgac ttcggcgtcg gcgtgtcatc gttcacgtac
     8341 ctcaagcacc tgccggtcga ctacctcaag atcgacggca gcttcgtgaa ggacatgctg
     8401 cgcgacccgg tgaaccacgc gatggtcgag gcgatccacc gcatcggcca catcatgggc
     8461 aagatgacga tcgccgagtc ggtcgagcat cgcgacacgc tgaaggcgct ccgcgcgatc
     8521 ggcgtcgact acgcgcaggg cttcgggatc gcccggccgg caccgttcgg ccgcatgcgc
     8581 gcggtgcgca ccggcccgca gaagcgccag gcggccggcg gcgcgtaacc gcctgtaccg
     8641 caatcggcgt gcgcctgccc ggcgcgcggc gtttccgagg gattttcgaa ggacgcgcgc
     8701 agtccatcct tcacggggcg ccggctttgc tttccagcag atagtctcgg tctatgcagc
     8761 cgatgcaata attaaatttg cgttatcgac tggcggaccc caccatccct ccatgccaac
     8821 cgcaaccgcg aaggagaaaa tcgacatggc caataccgtt tcgacccgcg aaggtcaccg
     8881 tgttcccgac gtctcgttcc gcattcgccg taacggcgac tgggcgaccc tgaccacgca
     8941 ggacatcttc gccggcaagg acgtcgtcgt gttctcgctg cccggcgcct tcacgccgac
     9001 gtgctcgtcg acacacctgc cgcgctacaa cgagctcgcg cccgtcttca aggcgcaagg
     9061 catcgacacg atcgtctgcg tttcggtgaa cgatccgttc gtgatggagg aatggggcaa
     9121 ggaccaggaa gccggcaacg tcgtgctgct gcccgacggc aacggcgagt tcaccggcaa
     9181 gatggggatg ctcgtcgaca agaccgacct caacttcggc aagcgctcgt ggcgctactc
     9241 gatgctcgtg cgcgacggca cgatcgagaa gatgttcgtc gagccgcagg agccgggcga
     9301 tccgttcaag gtgtccgatg ccgacacgat gctccgctac atcaatccga acgcgaagaa
     9361 gcccgaccag gtcgcgatcc tgacccgcga cggctgctcg ttctgcgcga aggcgaagga
     9421 gcgccttgcc gatgcgggct acgattacgt cgaggtgccg ctgccgcata cgatccgcac
     9481 caaggccgtc ggggcgatcg cgggtgcgca aacggtgccg caggtgttcg tcaacggccg
     9541 gctgatcggc ggaacgaacg agctcgacgc gttcctgcgc aaggccgcat gaccacattt
     9601 tccaccgacg ttgccgtaat cggcgcgggc accgcggggc tcgcagcgta tcgtgctgcc
     9661 aaggccgccg gccgcacggc gctgctgatc gagggcggca cctacgggac cacctgcgcg
     9721 cgcgtcggct gcatgccgag caagctcctc atcgcgcccg ccgaggccgc gcacgcgctg
     9781 gcgcgcttcg atgcgttcgg cctgcggctg ccaggcgagg tcgtcgtcga cggcgaggcg
     9841 gtgatggcgc gcgtgcgcgc cgagcgcgac cgcttcgtcg gcttcgtgct gcagggcgtg
     9901 gccgccatcc cgcccgccga cagaatggac gcctgcgcgc gcttcgtcga cgatacgacg
     9961 ctcgtcgcgg ccgatgcgac gatcactgcc ggcgcggtgg tgatcgcgac cggctcatcg
    10021 ccggcctacc ccgcggcgtg gacggcgctg ggtgagcggc tcgtgacgaa cgaaacgatc
    10081 ttcgattgga cacggctgcc ggaaagcgtc gtcgtgttcg gacccggcgt catcgggctc
    10141 gagctcggac aggcgctgca tcggctcggc gttcgcgtga aggttttcgg gcgcggcggc
    10201 ggtgtcggcc cgctttccga tcccgagctg ctggccgagg cgcggcgact ctttgcggcg
    10261 gagttctacc tcgacaccga cgcgcgggcc gaggtgacac gcgatggcga tcgtgtcgtc
    10321 gtccgctacc gcccccccga cggcgagctg cgcaccgagc ggttcgacta tctgctcgcg
    10381 gcaacgggac gcacgccgaa cgtgcgcacg ctgcggctcg aagcgacgac gctcgcgctc
    10441 gatgcgcaag gcgtgccgct gttcgaccgc gagacgctgc aatgcggaag cgcgccgatc
    10501 ttcatcgccg gtgacgcgaa cggcgacgtg ccgttgctgc acgaggcggc cgacgagggc
    10561 cgcatcgccg gcgagaacgc ggcgcgcttt cccgacgtcc atgccggcct gcgccgggcc
    10621 gggctcgcca tcgtgttcac cgatccgcag atcgcggtga tcgggggcgg ccttcgcgcc
    10681 gcgcgcgagg cggcggccgg gttcgtggcc gggaccgttt ccttcgagga tcaggggcgt
    10741 gcacgggtga tgcagcgcaa tcgcggacgc ctgcacgttt atgccgaaat cggcagcggg
    10801 ctgctgctgg gtgccgaaat gatcggcccc gacgccgagc atctcgcgca tctgatcgcc
    10861 tgggcgcgac agctcgatct caccatcggc gagatgctga cgctgccgtt ttatcatccg
    10921 gtggtcgagg aaggcttgcg caccgcgctg cgtgacgcat acgcgaagct cgacgccgca
    10981 tccaacgagc gccgcgccgc ctgataaggt tccggcggct cagcgcagca ggaagtacag
    11041 cgcgtagacg atcgcaatga gcacgaaaat caccgccggc accaacacct tgcgcgtgcg
    11101 cagcgggtgc ggacggtcgt agacgccgac cgtgccgggc gaacggccat tgcggttagc
    11161 tttcacgatc ggcgttccga agatcggtgc agaaccggac gccgggcacc tcgcggtgcc
    11221 cggcctggcc gcgcgtcatt acgtgcccgc gcccggcgtt ctcggcgtgg tgcggtcgcc
    11281 ggccgacccc gcggtatcgc ggtcgggaat cgagccggcc ggtccgccgg tgagtacgcc
    11341 accgatggag gcgccactcg cgatgcccgt ctcgcggtcg cgccgaccgt aaaggtcgcg
    11401 atcgcggtcg agatccgcgg gcgtatacgg ctgcgccttt tcgtcgaagc cggtccagcc
    11461 gcgctcgcgc cactgctcgg cgcgccgctc gatgtcgatc gcaccgtgcg agcgcatgat
    11521 ctccgccgca cgatcggcac gcgcgtcgtc ggcacgcacg gtgacgagcg caccgccgcg
    11581 acgtaccgct tcggcgtaat actcggcgtc cggccgcgat acgcccatgt cggtgaggcc
    11641 cccgatcagc ccgccggcca cggcaccgac gcctgccccg gtgaggagcg aaacaatggg
    11701 tcccgcggcg atgatcgggc cgatgcccgg gatcgcgagg cccatgaggc tcgccgccag
    11761 gcctgccgca ccgccgacga cgccgccggt caccgcaccc gccgccgcat tgctgccgtc
    11821 atcgacccga tcgtcgacgc cgcgatggcg atcgatggcg gcgtcggtca ggccggcacc
    11881 atcctcgctg acggcattgt cggccgccga gcgcgtgtag tgctcgctcg acgtggtgcc
    11941 gcgcgccgcc gtgccgccgc tcgcggtgcc catccccgcc atcgtgccgg cgcccgtgtc
    12001 gttggtgaac ccggtcgacg tgcgcgttcc gcgggcgccg agcatgcttt ccgttcccgc
    12061 gacgccggtc gatgtgtcgg ccggtgcgct aacgccggca ccgctcgacg tgcccgttcc
    12121 gctgcgataa tcgttgcgaa cgttgctcgc gacgacactc acgtcgctgt cgcggaaacc
    12181 gtcgtccatc aactcgcgcg ccacccggtt cgcttcgtca aaactgtcga acgaccctac
    12241 gattgtcttc atgccaatgg tccccgcgtc ggaaaatcat cgaacgtgcg accagccgcc
    12301 gcacgcgtca tgcggtgggc aggcactgca atccttcgcc cgccgcgatg tccgccgtac
    12361 tgtaatcacc atccgccgcg ctgtagagcg gccggccgcg cgaacgcctg tgcgccgcgt
    12421 cctacttcgc tgtcctacag cgcgcccttt acagcggcag gacagccacc gacaatgcac
    12481 gccgtcctga cgcagggagc acgggtcgtg ttttcgattc tcattcggtg gctatgcgcc
    12541 gccgccgccg cgacactggc gctcgccggc gtcgcgagcg cgcaggacta cccggcgcgg
    12601 ccgatccgcg tgatcgtgcc gttctcgccc ggcggagcgg tcgatggacc gatgcggctc
    12661 atcgcgcagg agctcggcaa gcggcttggc cagccggtgg tcgtcgagaa caagccgggt
    12721 gccggggcaa ccatcggttc ggacatcgtg gcgaagtcgc ccgcggacgg ttacacgctg
    12781 ctgctcgcat cgcagacgaa cgcaatcagc gcaacgcttt atgcgaagct gccgttcgat
    12841 ccgatcggcg acttcacgcc cgtgacgctg atcggacgcg agcccggcgt cgtcgtcgtg
    12901 aacccggccg ttcccgcaaa aacgctgcac gagttcatcg cgtacgtgaa gcagcatcct
    12961 ggcgaagtga actacgcgtc gtcgggcaac ggcagcgggc agcacctttt cgcggccctc
    13021 ctcgcctcgc gcaccggcat gaagatgaat cacgtcccgt accgcggcag cggacaggcg
    13081 acgaccgacc tcttgtccgg tgtcgtggcg atggcgatcc cggggaccgc cgggatggtc
    13141 ggacacatca aggcaggtaa gcttcgggcg ctcgccgtca cgggcgccgg gcgctcgccg
    13201 caattacccg acgtgccgac ggtggccgaa tcgggcgtgc aaggctacga ggcgtacgtg
    13261 tggatgggcc tgctcgcgcc gaagggcacg ccgccttcga tcatcgagcg gctcaaccgc
    13321 gacgtcgtcg cggtgctcgg cgaggaccag gtgaagcgct acatggcgaa tgcgggcatc
    13381 gagatcgtcg gctcgacgag tgcggatttc ggccgtttct ttcgcgccga gcgcgaccag
    13441 tgggccaagg tgatccacga cacgggcgcg cacatcgaat gagttcagat ggggacatga
    13501 gcgagtcgcg ccagcgcacg tcgacgatcg acgcgccggt ggaaggcgtc gcgcacgaca
    13561 cgcacaaggg cgacgtgcgc ctcttcctgt gggaaaagtt cgtcggcgag ccgtcgggca
    13621 agcccgtcgt gctgttcgtg cacggctcgt cgatggcgtc gcagccgacg ttcgacctga
    13681 cggtgcccgg ccggcccgac tcgtcggtga tggactggtt cgcacggcgc ggctttgcgt
    13741 gctggagcgt cgacatggaa ggctacggac gctcggacaa gcgccgcgac attgcctgcg
    13801 acatcgccaa cggcgccgac gatctcgcgg cggcgacgaa ttacatgcgc gacgcccgcg
    13861 gcatcgaatc cgtgatggcg tatggcatct cgtcgggagc gctgcgcgcg gcgctcttcg
    13921 ccgagcgcca tccgcggcgc gtggcgcgcc tcgcgctcga cgcgttcgtc tggaccggcg
    13981 aaggcgcgcc gacgctcgag cagcggcgca gcaagctgcc cgagttcagg gcgcgcaacc
    14041 gccgcccgat cgaccgcgcg ttcgtcgagt cgatctttgc gcgcgaccat ccgggttgcg
    14101 ccgagccggg cgtcgtcgcc gcgttcgccg acgccatcct ggcgctcgac gattcgatgc
    14161 cgaacggcac gtacgtcgac atgtgctcga agctgccggt cgtcgacccg gcgaaaatcg
    14221 cgatgccgac gctcatcatg cgcggccagt acgacggcat cgccgcgctc gacgacctga
    14281 tcgaattctt ccggcggctg cccaatgccg acaagcagtt cgcgatgatg cccggcatct
    14341 cgcacgcgag cttccagcag aagaactacc gcgttgccta ccacatcctg cacgcgtttt
    14401 tcacccagcc cgcgcccgtc tacggcggcg agtagcgaag cgccgcaccc gtcatggccg
    14461 tccaacccac gctgctgctg attgcggata tcgccggcta cacgcggttc atgaagatgc
    14521 acgacaccag tctcgtccac gcgcagcaga tcatcgccga tttgctcgag gcggtgatcg
    14581 acgccgccgg accgagcctg cggctcgcga agctcgaagg cgacgcggcg ttcttctaca
    14641 tgccgtcgcc cgcagacggt gcgccggcgc cggcgctgat cgaggaacgt accgctgcaa
    14701 tctaccgcgc gttccatgca cgcgccgccg acatcaaggc gaacacgctg tgtccttgcg
    14761 atggatgcca gcaggcgggc aatctcaaga tcaagctcgt cggacacgca ggcggcgtgg
    14821 ccgtccagaa ggtcaagcgc ctgaccgagc tcgccggcgt cgacgtgatc gtcgtgcacc
    14881 ggatgctgaa gaacgacgtg ccggtgtccg aatacctgct cgtgacgaag cccgtccacg
    14941 cgatgctcgg cgacgcattg cgcgagcgcg cggccgcatt cgatctcgag ctcgacgatc
    15001 tcggcaagac ttccgcctgg tatatcgatc ttgccgactg cgtggcgccg cttccgccgg
    15061 cacgcaagcg ctcgccgctc gcgcgggtcg gcggactgct gcgcttcgag gtgcgcaacc
    15121 tcccgtacgc gctgcacctg cgcaaggccc gcgtcggatt tcggaacctc cccgatgcgc
    15181 attgaatgcg cgcgttcgcg caacgcgcac gcgctgcccc gaggccgccg gtgaccgacc
    15241 tcgtcgaggt gcgcgacctg tcgatcgaat tccagacgcg cggactcacg attcgcgcgc
    15301 tcgaccgcgt atcgtttcgc atcgcgccag gcacgacgct ggcgctcgtg ggcgaatcgg
    15361 gctcgggcaa gtcgatcacc gcgcaggcga tgatgcgcat cctgcctccg agcgcaagca
    15421 tcacgtcggg gcagatcgtg ttcacgcagc ccgacggtcg cgaacgcccg gtcgacatcg
    15481 tccggctcgc gcccgacgga cgcaagatcc ggcgtatccg cggccggcac atctcgatga
    15541 tcttccagga gccgatggtg tcgctgtcgc cgttgcacac gatcggcgac caggtgagcg
    15601 aagcgctgtt cctgcatcat cggatcgggc accgcgaagg gatggcgcgc accgaagaca
    15661 tgctgcggcg cgtcggattt cccgatcccc accgcgcgct tcgcacgtat ccgttccagc
    15721 tatccggtgg actgcgccag cgcgcgatga ttgcgatggc gctcgtgtgc cgcccgtcgc
    15781 tgctgatcgc cgacgagccg accaccgcgc tcgacgtcac gatccaggcg cagatcctcg
    15841 ccctcgtcaa ggagctgcag gccgagctcg gcatggcggt cctcatcatc acgcacgatc
    15901 tcggcgtcgt cgcgaacgtg gccgagcacg tcgtcgtcct gtatcaggga cgcgtgatgg
    15961 aacagggccg cgtcgaggcg atcttcgagc gtccggcgca tccttatctg cgcgcgctgt
    16021 tgcgtgccgt tccgcacttc gacatgaagc ccggcgagcg gctcgtgtcg gtgcgaagcg
    16081 tcgactacca gacgggaccg ctcgtcgaga cgcgcgaagc gtggcccgca ggcgccgacg
    16141 cgccgcacct caccgtgcaa agcgtcgtca agacgttcgc gcagggcacg agcggctttc
    16201 tcgttcgccg cgagggtccg gcgtttcgcg cggtcgacga cgtgagcctc gcaatccgcc
    16261 gcggccagtg cttcggcctc gtcggcgaaa gcggctgcgg aaagacgacg ctgtcgaagg
    16321 tcatcatgcg ggcgatggag cccgacagcg gcaaggtgct ctacaacgat cgcggcacga
    16381 cgatcgacgt gacccgtgcg agcggcgata cgctgatgca gttccgccgc cgcgtgcagt
    16441 acatcttcca ggacccgttc ggctcgcttt cgccgcgcat gaccgtgttc gacatcctgc
    16501 gcgagccgct cgtcatccac ggcatcggcg acgcggacta ccgcgcggag atggcgaagg
    16561 agctgatgcg gctggtgggt ctcgacccgc ggtacctgag ccgctatccg cacagtttct
    16621 ccggcgggca gcgccagcga atcggcatcg cgcgtgcgct cgcgctgtcg cccgacctca
    16681 ttctttgcga cgagccggtt tcggcgctcg acgtgtcgat ccaggcgcag atcctgaacc
    16741 tgctgaagga cctgcaggcg gagctcggcc tcacttacct cttcatctcg cacaacctcg
    16801 ccgtggtcga ctacgtcgcc gacgagatcg cggtcatgtg ccgcggtcgc atcgtcgagc
    16861 tcgccccgcg cgaggtgctg ttccgcgacc cgatgcatcc gtatacgctc ggactgctcg
    16921 cggccgtccc gtatcccgat ctgcggcgca agctcgacct cgccgccgtg caggcgggcg
    16981 cggcgcgcag ccccgacgaa tggccggcgc cattcgcgtg ggccgacgcg aatgccggcg
    17041 agctggtgga cgtcggcgac ggccatcgcg tgcggatgca gacggccgcg gcagcgcgcg
    17101 aaccggtgac gacatgaggc ctgttatcga gctttacata attgcgtgac gtcgggcacc
    17161 cgaagcttcg tcgtccccgc gaaggtgggg acccagtggc gcattcgagc gaaagacgct
    17221 aggttcccgc ttccgtggga acgacggagc tctgcaaatt gtgatggaat tccgtaatgc
    17281 tcaataacgc catggccgcc gcccggctgc tcctcgtcgc gctcgtcgtg ctgttcgccg
    17341 cacctgcgct ggccgaggag ccgccgtcgc tcgcggccga cgttgccgcg gggaagctgc
    17401 ctccgctggc cgagcgcctg ccgcgcgcac cgttcgtcgt cgacgtcgca cgcgcgggct
    17461 ggtcgccggg ccgctacggc ggcgaactgc gcatgctgat ggcgaaggat cgcgacatcc
    17521 gcatgatggt cgtttacggc tacgcgcggc tcgtcggata cgacgagcat ctgcatctgg
    17581 tgcccgacat cctgcaaagc ctcgacaacg acggcaaccg cgtgtttacg ttgcatctgc
    17641 ggcccggcca caagtggtcg gacggacagc cgttcaccgc cgacgatttc cgctatttct
    17701 gggaggacgt cgcgaacaac aaggatctgt cgccgttcgg gttgccgcgc gcgctgctcg
    17761 tcgacaatca cgggccgaag ttcgaggtgc tcgacgccac gacggtccgc tacacgtggg
    17821 acgagccgaa cccgcagttc ctgaccgcac tcgcgggccc gagcccgctg ttcatctatc
    17881 ggcccgcgca ctatctcaag caattccacg cgcgctacgc cgggctcgac aaggccaatg
    17941 cacgcgccgt cgccgccggt tcgcgcaact gggcgggatt gcaccagaag atggacgagc
    18001 agttccgctt cgacaatccc gacctgccca cgctcgagcc gtggatcaac acgaccgggc
    18061 tgccgacgac gcgcttcgtg ctcgtgcgca atccctattt ccaccgtgtc gaccggatgg
    18121 gccaccagct tccgtacatc gaccgcgtga ttgtcggcgt caccgacgac aagctgatcc
    18181 ccgcgcgcac cggtgcgggc aatgtcgatt tgcaggcgcg ctacctgcgc ttcgacaact
    18241 acacgttcct gaagcaggcc gagacgcgca accggtaccg cgtctacctg tgggaaaagg
    18301 cgatcggctc gcaggtggcg ctctatccga acctgaatgt cgacgatccg gcgtggcgga
    18361 cactgatgcg cgacgtgcgc tttcgccgcg cgctgtcgct cggcatcgat cgccacgaga
    18421 tcaacgaggt cgtctatttc ggcctcggcc aggagtcggc gaacacggtg ctgccgagaa
    18481 gcgcgctcta ccgtcccgag tttcgcgccg cgtgggccgc gttcgatccg aagcgcgcca
    18541 acgcgctgct cgacgcgatc ggcctcgcca agcgcgatgc gaatggcatc cggctcctgc
    18601 ccgatgggcg tccgatggag ctcatcatcg acacgtcggg cgaaagcacc gaggaaaccg
    18661 acgtgctgga gctgattcgc gatacctggc gccagctcgg catcgcgatg ttcgcgcggc
    18721 cgtcgcagcg cgaggtgttc aggaagcgcg tgttctccgg caaatcgatg atgtcggtct
    18781 ggtcgggcct caacaacggc gtgccgacgc ccgagatgag cccctccgag cttgcgccca
    18841 cggcgcagga gcaactgcaa tggccgatgt ggggacagta ctacgagcag aaccgcaacg
    18901 gcggcgaaaa gccggcgctg ccggaagtcc aggagctcgg gcggctatac gacgaatggc
    18961 gccgcgccac caacgacgac gagcgcgagc gcatctggct gcgcatgctc gcgatccacg
    19021 ccgaccaggt ctacacgatc ggcaccgtca cgcatgcgct gcagccggtc gtcgtcagcg
    19081 atgacctgcg caacgtgccc tccgagggca tttacagctg ggatccgggc gcgtacttcg
    19141 gcatgtacca caccgacgcg ttctggttcg gcaccgaggc gcgctgatgc tccagtacat
    19201 caccaagcgc atcctgatga tgatcccgac gctgttcgcg atcagcatca tcgtgttcac
    19261 gatcatccag cttccgcccg gaaactacct cgagagctac atcgccgagc tcgaggcgca
    19321 gggcgaatcg gtcgaccagc agaaagtcgc gttcctgcgc gagtactacg gcctcgacaa
    19381 gccgcttccc gagcaatacc tgcattgggc gggcgggatg ctgaagggcg acttcggcta
    19441 ttcgttcgaa ttcaacatgc cggtgacgaa ggtgatcggc gaccggatgc tgctgacggt
    19501 catcgtttcg ttcgtcacga tccttttcac gtgggccgtc gcgtttccga tcggcatcta
    19561 ctcggcaacg catcaataca gctggggcga ttacggattg tcgttcctcg gct
//
LOCUS       DASSVX010000087        12985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1152868, whole genome shotgun sequence.
ACCESSION   DASSVX010000087 DASSVX010000000
VERSION     DASSVX010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12985
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152868"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /gene="mltB"
                     /locus_tag="VFO53_03985"
     CDS             <1..1045
                     /gene="mltB"
                     /locus_tag="VFO53_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HET8875468.1"
                     /translation="AIFTALPSAAASRSHTQASAQPGSGYERREDVRAFIDALVRDDG
                     FSRKTLTRWFRDVRYQPKIVEAMDRPISAPPKWFEYAPQFLTPERIDGGVAFWQAHAD
                     ALARAERDFGVPPEIVVAIIGVETFYGRNVGSYRVIDALSTLAFDYPRRAAFFRGELR
                     QFLLLAREEKFSPLVPKGSFAGAFGVAQFMPGSVRAYAIDYDGDGRIDLWQSGDDAVG
                     SIANYLARHDWLRGQPIWSKAMIASSQREAVLRRLDGGLSERRPLEAWNADGVAAERL
                     PDPMSPGPVGLLALEAADADGDTDAQDLRIVFPNFYVITRYNKSRLYASAVTSLAEAI
                     RAQHDGADGSAPR"
     gene            complement(1031..2692)
                     /gene="pgi"
                     /locus_tag="VFO53_03990"
     CDS             complement(1031..2692)
                     /gene="pgi"
                     /locus_tag="VFO53_03990"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET8875469.1"
                     /translation="MHVDPSSASVDLASCARRLKATTLHELFACDAGRVQALTLGWND
                     WHADLSKERVDADALDALLRTAERANLAHWIAALFAGEKINVSEHRAVLHTALRAPAS
                     TSIYVDGVDVTARMRETRERMAAITAAIREGRRKGATGRPVRFVINIGIGGSDLGPRL
                     VCEALDGSEDARSGAPGAGFISNVDPEHATRVLAGRDPASTLFVVTSKTFTTQETLAN
                     AHTAREWLRRGLGDDRDLAPHFIGVTSNTDAARQFGVRPDDILPMAEGVGGRYSLWSA
                     VGLVIAIRAGWKAYSDLLDGAQAMDTHFLMAPLARNLPVVLGLVGYWNARWLGHRQRL
                     VVPYAQALARLPAYLQQLSLESNGKSVRRDGTPVDATTAPALWGDVGTDSQHAFFQWL
                     HQGTQEVPVEFIVPVRAAHPLGDQQTLLVANALAQAQALMAGHGELALRNELSTRGLA
                     GGELDAAVAARRCPGNRASTTLLLPQLDARNLGALLSLYEHRTFVEGMLYGINSFDQW
                     GVELGKTLAKPIVSALVDAVPLPDDTDGSTRELVARARSLASARR"
     gene            2877..4445
                     /locus_tag="VFO53_03995"
     CDS             2877..4445
                     /locus_tag="VFO53_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8875470.1"
                     /translation="MALAAALVLAVALGAPGALAQGQKVLKFIPQADLRILDPITTTA
                     YITRNHGYMIYDTLFATDAKFQVQPQMVDKYELSPDKLTYTFTLRDGLKFHDGAPVRS
                     ADCIASVERWAKRDALGQKLAEATQAWKAVNDKTFELKLKKPFPLALEALAKPSSNVP
                     FIMPERIAKTDASTNITEAIGSGPFKFVKSEWVPGNKVVYVKNTDYVPRKEPPSWAAG
                     GKVVKVDRVEWIYIPDSATAAAAINAGEVDWWEQMPPDLIPVLAKNKDVKVENIDPLG
                     SMGVLRFNFLYPPFNNQKMRQALLYAIDQNDYVLGIAGDMKNGHPCYSYFTCGTPLAS
                     EVGAGPMKGKRDFDKAKALIKEAGYKGEKIVIIDATDQPIVHSQSLLTAEALRKLGLN
                     VEVQAGDWGTLITRRSSKEPIDKGGWSIFHTWLVGPDVTSPAVNFALRGSGDKAWFGW
                     PTDAKLEELREAWFAASDPAASKTAADAVQARAFDFVPYIPTAQFILPTAYRTNLSGV
                     IIAPVTFMWNVEKK"
     gene            4489..5430
                     /locus_tag="VFO53_04000"
     CDS             4489..5430
                     /locus_tag="VFO53_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875471.1"
                     /translation="MIRYVIRRLLSTVVVMAVVAFVVFSLLYLTPGDPAAIIAGDIAT
                     DEDIHRIHERLGLDDPFAVQFGRWVRALAHGDLGTSIFTNLPVTQLIGQRIEPTVSLT
                     LCTLVVSIALALPLGVLAAAKAGTWLDRAVMAFSVLGFSVPVFVLAYILILTFSVHLD
                     WLPVQGYRNFRDGAWEWLRHLILPSIALGTVYVALITRITRASMLDVLAQDYVRTAQA
                     KGLAPGDVLIGHALKNAAVPVMTIIGIGVALLISGAIVTETVFALPGIGRLTVDAILR
                     RDYPIIQGVTLLFSAVYVLVNLVVDLSYALFDPRIRY"
     gene            5450..6340
                     /locus_tag="VFO53_04005"
     CDS             5450..6340
                     /locus_tag="VFO53_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875472.1"
                     /translation="MATALPAESIAVGRSLSPTWARFRDAFRRHPMALVGGAILAGMV
                     LVAVLAPWLGTIDPQAVSPVKRLRPPSAAYWFGTDMLGRDVYSRVVYGARVSLIVGLA
                     VAVVSTLLGVAIGLVTGYIRWLDAIVMRVMDGLMSIPSVLLAIALMALTRASVGNVIA
                     AITLAEVPRVVRLVRSLVLTLREQTYVEAAIAAGTTLPRILARHILPNIVAPLLVQGT
                     YVCGSAMITEAILSFIGAGTPPNIPSWGNIMAEGRSLFQIAGYLILFPGICLSLTVLA
                     VNLLGDGMRDALDPRLARRM"
     gene            6510..7568
                     /locus_tag="VFO53_04010"
     CDS             6510..7568
                     /locus_tag="VFO53_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875473.1"
                     /translation="MDALLEVDDLTTHFFTRDGVVRAVDGVSFRVMRGQVLAIVGESG
                     CGKSVTSLSIMRLIASPPGRTVKGRVLFEGRDLLELPEPEMRKIRGNAISMIFQEPMT
                     SLNPVLTIGRQVAEALILHRGMSREQALARSVELLQEVRIPAPERRVTQYPHQLSGGM
                     RQRVMIAMALACAPRLLIADEPTTALDVTIQAQILDLMRELSQRTGASIVLITHDLGV
                     VAEMAERVVVMYAGRKVEEASVAELFARPRHPYTRGLLDSIPKLRGAAVANTDGSGSR
                     RRLAEIAGTVPALSEPIVGCAFAPRCGFATARCRVESPLLEQKAPAHFAACWESDRMV
                     SATSTRPSQPRTRGVLHE"
     gene            7561..8574
                     /locus_tag="VFO53_04015"
     CDS             7561..8574
                     /locus_tag="VFO53_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET8875474.1"
                     /translation="MSDVRAGAPLLAVTHLTKHYAMRSGLFGGGRGAVHAVDDVSFTI
                     AEGETLGLVGESGCGKSTIARTVLGLTAPTAGAIAWRGERIDRLRAAAMRPYRQALQA
                     VFQDPYASLNPRMRAADIVAEPIRNFETASAGDVGARVLSLFQRVGLRADQMPRYPYE
                     FSGGQRQRLGIARALAPRPKLIVCDEPVSALDVSVQAQVINLLADLQDEFGLSYLFVA
                     HDLAVVEHISHRVAVMYLGCLVEVTTTRALFDRPLHPYTEALLSAVPVPDPAVRRKRL
                     ILAGDVPSPTELPSGCRFHTRCPYAFDRCRVDDPALVEAAPGHFVACHLREPAAAPSL
                     VSH"
     gene            8586..9713
                     /locus_tag="VFO53_04020"
     CDS             8586..9713
                     /locus_tag="VFO53_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET8875475.1"
                     /translation="MHHRPDAVALTKKLLAFNTINPPGAEEACARYLGDLLKSADFDV
                     DYAAFAPGRTSVVARIGGHDDRPSLGFTGHMDTVPLGGVPWKRDAFGASLEGGRLYGR
                     GASDMKSGVAAFVVAAMAQAGRLRESPGITLVLTADEECGCGGAAFLAQETDLLGSVG
                     ALLVGEPTANYPLVGHKGALWLEARASGVTAHGSMPERGDNAIHKAAQAVRTLQDFDF
                     GVEPHPLMGRPTLNIGTLHAGLNINSVPDRASIGIDIRTIPGQQHAGVRDRLQRALGH
                     AIEIAPIVDVEAIYSDPAAPWMQQVFGIAQAHLGERPQPRTATYFTDAAVLNAVYRDV
                     PICVLGPGEPQLAHQTDEYCLVDRVEQAVAIYDDLIRQWCF"
     gene            9701..10996
                     /locus_tag="VFO53_04025"
     CDS             9701..10996
                     /locus_tag="VFO53_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8875476.1"
                     /translation="MVLLMDHLNHLDATAAARKLATREITAEALLADCVERIGEREPV
                     VHAWTYLDVDAAMRRARLLDSQPASGLLYGLPIAVKDLFDTFDMPTACGSPIYAGHRP
                     AGDAASVALAREAGAIVVGKTVTTEFATFHPGPTCNPHNALHTPGGSSSGSAAAVADL
                     MVPLAFGTQTAGSIIRPAAYCGVVGFKPTFGTVTRIGVKMISDTLDTVGGFARTVPDV
                     ALLVAALSGRRSLLIDAPPAEAPRIGLCRTHEWPRAEPATVALFEDVERRLSARGAKV
                     RDVALPTPFAGLARAQTTIMVREVAQSLAHERLAHREALSADMIAMIDAGLAVSAEDY
                     DAARALARTCRAMLSAVFADVDVLVAPSTPGEAPAGTATGDPVFNRIWTLLRVPCVHV
                     PLARGPRGLPIGVTVAGNRDCDQRTLLAAAWIHEQFGSR"
     gene            10974..>12985
                     /locus_tag="VFO53_04030"
     CDS             10974..>12985
                     /locus_tag="VFO53_04030"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET8875477.1"
                     /translation="MNNSARAEIVDKDLPQKEDTRLLGRLLGDVLRGQLGEAGFDRIE
                     TIRQTAIGFRRSRGDDAERLRKALAELLNPLPIGAVLEVVRAFSYFSHLANIAEDVHQ
                     NRRRRAYALAGSPPRRGDIADALLRIAEAGVDRAAIERWLGEAIVSPVLTAHPTEVQR
                     KSILDVEREIARLLTWRDRTTLTPDEASDFAARLATSILGLWQTAMLRLSRLQVKDEI
                     DNGLAYYRYTFLDEIPHLYAAFEASVAGQFGVGRERVPAFLRMGSWIGGDRDGNPFVG
                     ADTLTYAIRAQAGVLFEHYLDDVHRLGGELSLSTRLVTPSAELLALAGAAHDANPHRQ
                     DEPYRQALIGVYARLAATAHELTDLSPARVVQTDAARYETAQAFRADLDTIAASLAQH
                     GAAPLAARRLEPLRRAVDVFGFHLAALDLRQNSDVHQAVVGELLARAQVAGDYSALQE
                     DERVALLVRELATSRLLHSPYLDYSERVGAELAVLRVAAGIHARYGADALPNYVISKC
                     QSVSDLLEVGVLLKEVGLLREHELAMNIVPLFETIDDLDRCGATMRAAFALPLYRTLV
                     ASRRDWQEIMLGYSDSNKDGGYLTSSWAIYRAERALVDATAAHDVRLRLFHGRGGTVG
                     RGGGPSYDAILAQPAGSVSGGLRLTEQGEIIASKYSDPELGRRNLEA"
BASE COUNT         1963 a         4383 c         4482 g         2157 t
ORIGIN      
        1 cgcgatcttc acggcgcttc cttccgccgc cgcatcgcgc agccacaccc aggcttccgc
       61 gcaacccgga tcgggctacg agcgcaggga ggacgtgcgc gcgttcatcg acgctctggt
      121 ccgcgacgac ggcttttcgc gcaaaacgct gacgcgctgg tttcgcgacg tccgctatca
      181 gccgaagatc gtcgaggcga tggatcgccc catctcggcg ccgccgaaat ggttcgaata
      241 cgcgccgcag tttctgacgc ccgagcgcat cgacggcggc gtcgcgttct ggcaggcgca
      301 cgccgatgcg cttgcgcgcg ccgagcgcga cttcggcgtg ccgccggaga tcgtcgtcgc
      361 gatcatcggc gtcgagacgt tctacggccg caacgtcggc agctaccgcg tcatcgacgc
      421 gctgtcgacg ctcgccttcg actatccgcg ccgtgccgcg ttctttcgcg gcgagctgcg
      481 gcaattcctg ctgctcgcgc gggaggagaa attctccccg ctcgtgccga agggctcgtt
      541 cgcgggagcg ttcggcgtag cgcagttcat gcctggcagc gtgcgcgcct atgccatcga
      601 ttatgacggc gacggccgca tcgacctgtg gcaaagcggc gacgatgcgg tcggcagcat
      661 cgcgaactac ctggcacggc acgattggct gcgcgggcag ccgatctggt cgaaggcgat
      721 gattgcgtca tcgcagcgcg aggcggtgct gcgcaggctc gacggcgggt tgagcgagcg
      781 gcgcccgctt gaagcatgga atgccgatgg cgtcgccgcg gagcggctgc ccgatccgat
      841 gtcgcccggg ccggtcggcc tcctcgcgct cgaggcggcc gatgccgatg gcgacaccga
      901 tgcgcaggac ttgcgcatcg tgtttccgaa tttctacgtg atcacgcgct acaacaagag
      961 ccggctctat gcgtcggccg tcacgtcgct cgccgaggcg attcgcgcgc agcacgacgg
     1021 cgcggacggc tcagcgccgc gctgaggcga gcgatcgggc gcgggcgacg agctcgcgcg
     1081 tcgacccgtc cgtatcgtcg gggagcggca ccgcgtcgac caacgccgac acgatcggct
     1141 tggcgagcgt cttgccgagc tcgacgcccc actggtcgaa cgagttgatg ccgtaaagca
     1201 tgccttcgac gaacgtgcgg tgctcgtaca gggagagcag cgcgccgaga ttgcgcgcgt
     1261 cgagctgcgg cagcagcaac gtcgtcgatg cgcgattgcc cgggcagcgc cgcgcggcga
     1321 cggcggcgtc gagctcgccg cctgcaaggc cccgcgtcga cagctcgttg cgcaacgcga
     1381 gctcgccgtg tccggccatc agcgcctgcg cctgcgctag tgcgttggcg acgagcagcg
     1441 tctgctggtc gccgagcggg tgcgccgcgc gcaccggcac gatgaattcg accggcactt
     1501 cctgcgttcc ctgatgcagc cactggaaga acgcgtgctg actgtcggtg ccgacatcgc
     1561 cccacagcgc gggagccgtc gtcgcgtcga cgggcgtgcc gtcgcggcgc acgctcttgc
     1621 cgttgctctc gagcgaaagc tgctgcagat aggcgggcag gcgcgcgagg gcctgcgcgt
     1681 acggcacgac caggcgctgg cggtggccga gccagcgcgc gttccagtaa ccgacgagcc
     1741 cgaggacgac cgggaggttg cgtgccaacg gcgccatgag gaagtgcgtg tccatcgcct
     1801 gcgcgccgtc gaggaggtcg ctgtacgcct tccagccggc gcggatggcg atgacgaggc
     1861 cgacggcgga ccacagcgaa tagcgcccgc ccacgccttc tgccatcggc aggatgtcgt
     1921 cggggcgcac gccgaactgc ctcgcggcgt cggtgttcga cgtgacgccg atgaagtgcg
     1981 gcgcgagatc gcgatcgtcg ccgagcccgc ggcgcagcca ctcgcgcgca gtgtgcgcat
     2041 tggcgagcgt ctcctgcgtc gtgaacgtct tggacgtgac gacgaagagc gtcgacgcag
     2101 ggtcgcgccc ggcgagcacg cgggtcgcat gctcggggtc gacgttggag atgaagccgg
     2161 cccccggcgc gccgcttcgt gcgtcctcgc tgccgtccag cgcttcgcac acgagccgcg
     2221 gtccgaggtc ggagccgccg atgccgatgt tgatgacgaa gcgcaccggg cgccccgtgg
     2281 cgcccttgcg gcggccttcg cgaatggcgg ccgtgatcgc cgccatgcgc tcgcgcgtct
     2341 cgcgcattct tgccgtgacg tccacgccgt cgacgtagat gctggtcgac gcgggtgcgc
     2401 gcagcgccgt gtgcagcacc gcgcgatgct cggaaacgtt gatcttttca ccggcgaaga
     2461 gcgcggcgat ccagtgcgcc aggttggccc gttctgcggt gcgaagcagc gcgtcgagcg
     2521 cgtcggcgtc gacgcgctcc ttggaaaggt cggcgtgcca gtcgttccag ccgagcgtca
     2581 gcgcctggac gcggccggca tcgcatgcga agagctcgtg gagcgtcgtc gccttcaggc
     2641 ggcgcgcaca gctcgcaaga tcgacggagg cggaggatgg atcgacgtgc atgggcgcgg
     2701 tgaggacgcg tgaacgcgcg cttgcgtgca atcctagcag gcgcgtggcc gccgggacgc
     2761 gggcggggct agaatgcacg ccacgttcag taccggaatc cgcagctcga aagagcgtgc
     2821 cgtcagccgc tttcccatcg gagacaacaa tgaccggacg taccgcgttt cgttccatgg
     2881 ctttggccgc cgcgctcgtg ctcgcggtgg cgctcggcgc accgggcgcg ctcgcgcagg
     2941 ggcaaaaggt gctgaagttc atcccgcagg ccgatctgcg catcctcgac ccgatcacga
     3001 cgaccgcgta catcacccgc aaccacgggt acatgatcta cgacacgctg ttcgccaccg
     3061 acgccaaatt ccaggtgcag ccgcagatgg tcgacaagta cgagctcagc ccggacaagc
     3121 tgacgtatac gttcacgctg cgcgacgggc tcaagttcca cgacggcgcg ccggtgcgct
     3181 ccgccgactg catcgcgtcg gtcgagcgct gggcgaagcg cgacgccctc ggccagaagc
     3241 tcgccgaggc gacgcaggcg tggaaggccg tgaacgacaa gacgttcgag ctcaagctga
     3301 agaagccgtt cccgctcgcg ctcgaggcgc tcgcgaagcc gtcgtcgaac gtgccgttca
     3361 tcatgcccga gcgcatcgcg aagaccgacg cgtcgacgaa catcaccgag gcgatcggct
     3421 cggggccgtt caagttcgtg aagtccgaat gggtgccggg caacaaggtc gtgtacgtga
     3481 agaacaccga ttacgtgccg cgcaaggagc cgccgtcgtg ggcggccggc ggcaaggtgg
     3541 tcaaggtgga ccgcgtcgaa tggatctaca ttcccgattc ggccaccgcg gcggcggcga
     3601 tcaacgccgg cgaggtcgac tggtgggagc agatgccgcc ggatctcatc ccggtgctcg
     3661 cgaagaacaa ggacgtgaag gtcgagaaca tcgacccgct cggatcgatg ggcgtgctcc
     3721 gcttcaattt cctgtatccg ccgttcaaca accagaagat gcgccaggcg ctgctgtacg
     3781 cgatcgacca gaatgattac gtgctgggca tcgccggcga catgaagaac ggccacccat
     3841 gctactcgta cttcacgtgc ggcacgccgc ttgcaagcga ggtcggcgcc gggccgatga
     3901 aaggcaagcg cgatttcgac aaggcgaagg cgctgatcaa ggaggcgggc tacaagggcg
     3961 agaagatcgt catcatcgac gctaccgacc agccgatcgt gcactcgcag tcgcttttga
     4021 ccgcggaggc gctgcgcaag ctcgggctca acgtcgaggt gcaggccggc gactggggca
     4081 cgctgatcac gcggcgctcg tcgaaggagc cgatcgacaa gggcggctgg tcgatcttcc
     4141 atacgtggct cgtcgggccc gacgtgacca gtccggcggt gaacttcgcg ctgcgcggct
     4201 cgggcgacaa ggcgtggttc ggctggccga ccgacgcgaa gctcgaggag ttgcgcgagg
     4261 cgtggttcgc ggcgagcgac ccggcggcgt cgaagacggc ggccgacgcg gtgcaggcgc
     4321 gcgcgttcga cttcgtgccg tacatcccca ctgcgcagtt catcctgccg accgcgtacc
     4381 ggacgaacct ctcgggcgtc atcatcgcgc cggtgacgtt catgtggaac gtggagaaga
     4441 aataggcttc gtcgcggtcc tgccgttggc gccgcgggct gacggcggat gatccggtac
     4501 gtcatccgcc gcctgctgtc gaccgtcgtc gtgatggcgg tcgtggcgtt cgtcgtgttc
     4561 tcgctgctgt acctgacgcc gggcgatccc gcagcgatca tcgcgggcga catcgccacc
     4621 gacgaggaca ttcaccgcat ccacgagcgc ctcggtctcg acgatccgtt cgccgtgcaa
     4681 ttcggccgct gggtgcgggc actcgcgcat ggcgacctcg gcacgtcgat cttcaccaat
     4741 ctgccggtca cgcaattgat cggacagcgc atcgagccga ccgtttcgct gacgctgtgc
     4801 acgctcgtcg tgtcgatcgc gctcgcgttg ccgctcggcg tgctggcggc ggcgaaggcc
     4861 ggcacctggc tcgatcgtgc ggtgatggcg ttctcggtgc tcggcttctc ggtgccggtg
     4921 ttcgtgctcg cctatatcct gatactcacg ttctccgtgc atctcgactg gctgccggtc
     4981 cagggctacc gcaactttcg cgacggcgcg tgggagtggc tgcggcatct gatcctgccg
     5041 agcatcgcgc tcggcaccgt gtacgtcgca ctcatcacgc gcatcacccg cgccagcatg
     5101 ctcgacgtgc tcgcgcagga ttacgtgcgc accgcgcagg cgaagggcct cgcccccggc
     5161 gacgtgctga tcggccacgc attgaagaac gcggccgtcc cggtgatgac gatcatcggc
     5221 atcggcgtgg cgctgctgat cagcggcgcg atcgtcaccg aaacggtatt cgcgctgccc
     5281 ggcatcggca ggctcaccgt cgatgcgatc ctgcgccgcg actacccgat catccagggc
     5341 gtgacgctgc tcttttccgc ggtctacgtc ctcgtcaatc tcgtcgtcga cctgtcgtac
     5401 gcgctgttcg atccgcgcat caggtattag ggcataactc cgcgttcgca tggcaaccgc
     5461 gcttcccgcc gaatcgatcg ccgttggccg atccctgtcg ccgacgtggg cgcgctttcg
     5521 cgacgcgttc cgccgtcacc cgatggcgct ggtcggcggc gccatcctcg ccgggatggt
     5581 gctcgtcgcc gtgctcgcac cctggctcgg aacgatcgat ccgcaagcgg tgtcgccggt
     5641 gaagcgcctg cggccgccgt ccgcggccta ctggttcggc accgacatgc tcgggcgcga
     5701 cgtctacagc cgcgtcgtgt acggcgcgcg cgtctcgctc atcgtcggac tcgccgtggc
     5761 ggtcgtgtcg acgctgctcg gcgttgccat cgggctcgtc accggctaca tccgctggct
     5821 cgatgcgatc gtgatgcgcg tgatggacgg actgatgtcg attccatccg tgctgctcgc
     5881 gatcgcgctg atggcgctca ccagggcaag cgtgggcaat gtgattgcgg cgatcacgct
     5941 cgccgaggtt ccgcgcgtcg tgcgcctggt gcgaagcctc gtgctgacgc tgcgcgagca
     6001 gacgtatgtc gaggcggcga tcgccgccgg cacgacgctg ccgcgcatcc tggcgcgcca
     6061 catcctgccc aatatcgtgg cgccgttgct cgtgcaggga acgtacgtct gcggctcggc
     6121 gatgatcacc gaggcgatcc tgtcgttcat cggcgccggc acgccgccca acattccgag
     6181 ctggggcaac atcatggccg aggggcgaag cctgttccag atcgccggtt acctgatcct
     6241 gtttcccggc atctgcctgt cgctgacggt gctcgccgtg aatctgctcg gtgacggcat
     6301 gcgcgatgcg ctcgatccgc gcctcgcgcg gcggatgtga catgagcgcc gccgggccgc
     6361 cccaaggcgc gaattgcgcc cccgtggggg gcagcgcagc gcgtgagccc gacgggccgt
     6421 cccgagggcg aacatctgag ggcgaagccc gaagcgtgtc tgacaagccg caagcgtggg
     6481 gggatcatac aagcgacacg agcgccggga tggatgcgct gctggaggtg gacgacctca
     6541 cgacgcattt cttcacgcgc gacggcgtcg tgcgcgccgt ggacggcgtt tcgtttcgcg
     6601 tcatgcgcgg ccaggtgctc gcgatcgtcg gcgaatcggg ctgcggcaag agcgtcactt
     6661 cgctgtcgat catgcgcctg atcgcgtcgc cgccggggag aaccgtcaag ggacgcgtgc
     6721 tgttcgaggg acgcgacctg ctcgagttgc ccgagcccga aatgcggaaa atccgcggca
     6781 acgcgatctc gatgatcttc caggagccga tgacttcgct caatcccgtg ctgacgatcg
     6841 gccgccaggt cgccgaagcg ctgatcctgc atcgcgggat gtcgcgcgag caggcgctgg
     6901 cacgttcggt cgagctgctg caggaagtgc ggataccggc gccggaacgg cgggtgacgc
     6961 agtatccgca ccagctttcc ggcggcatgc gccagcgcgt gatgatcgcg atggcgctcg
     7021 cctgtgcgcc gcggctcctg atcgccgacg agccaaccac cgcgctcgat gtcacgattc
     7081 aggcgcagat tctcgacctg atgcgcgagc tctcgcagag gacgggcgcg tcgatcgtgc
     7141 tcatcacgca cgaccttggc gtggtcgccg agatggcgga acgcgtcgtc gtgatgtacg
     7201 cggggcgcaa ggtcgaggag gcatcggtgg ccgagctctt tgcccgtccc cgtcatccct
     7261 atacgcgcgg cctgctggat tcgattccga agctgcgtgg cgcagccgtg gcgaacaccg
     7321 acggatccgg cagccggcgt cgcctcgcgg aaatcgctgg caccgtgccg gcgctttccg
     7381 agccgatcgt cggctgcgcc tttgcaccgc gctgcgggtt cgcgactgcg cgctgccgcg
     7441 tcgaatcgcc gctgctcgag cagaaggcgc cggcgcattt cgcggcctgc tgggaatcgg
     7501 atcgcatggt gagcgcaaca tcgacgcgac cgtcgcagcc gcgaacccgg ggcgtgctcc
     7561 atgagtgatg tgcgtgccgg cgcgccgctc ctcgcggtga cgcacctgac caagcattac
     7621 gcgatgcgca gcggcctctt cggtggcgga cgcggcgccg tgcacgccgt cgacgacgtg
     7681 agcttcacga tcgccgaggg cgagacgctc ggtctcgtcg gggagagcgg ctgcggcaag
     7741 tcgacgatcg cgcgcaccgt gctcggactc accgcgccaa cggccggggc aatcgcctgg
     7801 cgcggcgagc ggatcgaccg gctgcgcgcc gccgcgatgc gtccttaccg ccaggcgctg
     7861 caggccgtct tccaggatcc gtacgcatcg ctcaatccgc gcatgcgtgc agccgacatc
     7921 gtggccgagc cgatccgcaa tttcgaaacg gcgtcggccg gcgatgtcgg cgcgcgcgtg
     7981 ctgtcgctgt ttcagcgagt cggactgcgg gccgatcaaa tgccgcgtta tccgtacgaa
     8041 ttctccggcg ggcagcggca gcggctgggc atcgcgcgtg cgctcgctcc gcggccgaag
     8101 ctgatcgtct gcgacgagcc ggtctcggca ctcgacgtgt cggtgcaggc gcaggtcatc
     8161 aacctgctcg ccgatctgca ggatgaattc ggcttgtcgt atctgttcgt cgcccacgac
     8221 cttgcggtcg tcgagcacat cagccatcgc gtcgccgtca tgtatctcgg gtgcctcgtc
     8281 gaggtgacga cgacgcgggc gctcttcgat cgtccgctgc acccgtatac cgaggcgctg
     8341 ctgtccgcgg ttccggtgcc cgatcctgcc gtgcggcgca agcggctgat ccttgccggt
     8401 gacgttccaa gccccaccga gctgccctcc ggttgccgct ttcatacgcg ttgtccgtac
     8461 gcattcgacc gctgccgtgt cgacgatccc gcgctcgtcg aagcggcacc cggacacttc
     8521 gtcgcgtgtc acttgcgcga accggccgcg gcgccatcac tcgtttcgca ttgaagggcc
     8581 attccatgca ccatcgtccc gacgcggtcg cgctcacgaa aaagctgctg gcgttcaaca
     8641 cgatcaatcc gcccggcgcc gaagaggcgt gcgcgcgcta cctcggcgac ctcctgaagt
     8701 ccgcggattt cgacgtcgac tacgccgcgt tcgcgcctgg ccgaacgagc gtcgtcgcac
     8761 gcatcggcgg ccatgatgat cgtccctcgc tgggattcac cggccacatg gatacggtgc
     8821 cgctcggggg cgtgccgtgg aagcgggatg cattcggcgc gagtctcgag ggcggacggc
     8881 tctacggccg cggcgcgagc gacatgaaga gcggcgtcgc cgccttcgtc gtcgccgcca
     8941 tggcgcaggc cgggcggctg cgggaatcgc cgggcataac gctggtgctg accgccgacg
     9001 aggagtgcgg atgcggcggc gcggcattcc tcgcgcaaga gacggacctg ctgggaagcg
     9061 tcggcgcgct gctcgtcggt gaaccgacgg cgaattaccc gctcgtcggg cacaagggcg
     9121 cgttgtggct ggaggcgcgc gcgtcgggcg tgaccgcgca cggctcgatg cccgagcgcg
     9181 gcgacaatgc gatccacaag gcggcgcaag cggtgcgcac gttgcaggat ttcgatttcg
     9241 gcgtcgagcc gcatccgctg atgggcaggc ccacgctcaa catcggcacg ctgcacgccg
     9301 gcctcaacat caactcggtt cccgatcggg cgtcgatcgg catcgacatc cgtaccattc
     9361 ccggccagca gcacgccggt gtgcgcgacc gtttgcagcg cgcgctcgga cacgcaatcg
     9421 agatcgcgcc gatcgtcgac gtcgaggcga tctactccga tcccgctgcg ccctggatgc
     9481 agcaggtgtt cggcattgca caggcgcacc tcggcgagcg tccgcaaccg cgcacggcga
     9541 cctatttcac cgacgccgcg gtgctcaatg cggtctatcg cgacgtgccg atctgcgtgc
     9601 tgggtccggg cgagccgcag cttgcgcatc agaccgacga atactgcctc gtcgatcgtg
     9661 tcgagcaggc ggtggccatc tacgacgatc tgatccggca atggtgcttc tgatggatca
     9721 ccttaaccac ctcgatgcaa cggccgcggc gcgcaagctc gcgacccgcg aaatcactgc
     9781 ggaagcgctg ctcgccgatt gcgtcgagcg catcggcgag cgcgagccgg tcgtccacgc
     9841 ctggacgtat ctcgatgtcg atgcggcgat gcgtcgcgcg cgcctgctcg attcgcagcc
     9901 ggcgtcgggt ctgttgtacg ggcttcccat cgcggtcaag gatctgtttg acacgttcga
     9961 catgcccacc gcctgcggct cgccgatcta tgccgggcat cggccggcag gtgatgcggc
    10021 gtcggttgcg ctcgcccggg aagcgggcgc catcgtcgtc ggcaaaacgg tgacgacgga
    10081 attcgcgacg tttcatcccg ggccgacgtg caatccgcac aatgcgttgc acacaccggg
    10141 cggctcgtcg tcgggctcgg cggcggcggt ggcggatttg atggttccgc tggcgttcgg
    10201 cacgcaaacg gccgggtcga tcatccggcc ggcggcgtat tgcggcgtcg tcggtttcaa
    10261 gccgacgttc ggcaccgtca cccgcattgg cgtgaagatg atctccgata cgctcgacac
    10321 cgtcggcggg ttcgcccgca ccgtgcccga cgtggcgctg ctcgtcgccg cactgagcgg
    10381 acgccgttcg ctgctgatcg acgcgccgcc cgccgaggca ccgcgcatcg ggttgtgcag
    10441 gacgcacgag tggccgcgcg cggagcctgc aaccgtcgcg ctgttcgagg acgtcgagcg
    10501 gcggctgtcc gcccgcgggg cgaaagttcg cgacgtcgcg ctgcccacgc cgttcgccgg
    10561 tctcgcccgc gcgcagacga cgatcatggt gcgcgaggtg gcgcagtcgc tggcgcacga
    10621 acggctcgcg caccgcgagg ccctgagtgc cgacatgatc gcaatgatcg acgcgggact
    10681 cgcggtttcg gcagaagact acgacgccgc acgcgcattg gcgcgcacat gccgcgcgat
    10741 gctgtccgcg gtgttcgccg atgtcgacgt gctcgtcgcg ccaagcacgc ctggcgaggc
    10801 gccggccgga acggccaccg gcgatcccgt attcaatcgc atctggacgc tgctgcgcgt
    10861 gccgtgcgtg cacgtgccgc tcgcacgcgg cccgcgcggg ctgccgatcg gcgtcaccgt
    10921 cgcaggcaat cgcgattgcg accagaggac gctgctcgca gcggcatgga ttcatgaaca
    10981 attcggctcg cgctgaaata gtcgacaagg acctgccgca gaaggaggac acgcgcctgc
    11041 ttggacggct gctgggcgac gtgctgcgcg ggcagctcgg cgaagccggt ttcgatcgca
    11101 tcgagacgat tcgccagacg gcaatcggct ttcgccgctc gcgcggcgac gacgccgagc
    11161 gcttgcgcaa ggcgctcgcg gaactgctca atccgctgcc gattggcgca gtgctcgagg
    11221 tcgttcgcgc attcagctat ttttcgcatc ttgcgaacat cgccgaggac gtgcaccaga
    11281 accggcgccg gcgtgcctac gcgctcgcgg gctcgccgcc gcgccgcggc gacattgcgg
    11341 atgcgttgct gcgcatcgcc gaggcagggg tggatcgcgc cgcgatcgag cgctggctcg
    11401 gcgaagcgat cgtcagcccc gtgctgaccg cgcatccgac cgaagtgcaa cggaagagca
    11461 tcctcgacgt cgagcgcgag atcgcgcgcc tgctgacctg gcgcgaccgc acgacgctca
    11521 cacccgacga agcgtcggat ttcgcggcgc gccttgccac gtcgatcctc ggcctgtggc
    11581 aaaccgcgat gctgcgcctg tcacgtctgc aggtgaagga cgaaatcgac aacgggctcg
    11641 cgtattaccg ctacacgttt ctcgacgaga tcccgcacct gtacgcggcg ttcgaagcaa
    11701 gcgtcgccgg gcaattcggc gtcggacgcg aacgcgtgcc ggcgttcctg cggatgggct
    11761 cgtggatcgg cggcgaccgc gacggcaatc cgttcgttgg cgccgatacg ctgacgtacg
    11821 cgatccgcgc gcaggccggc gtgctgttcg agcactatct cgacgacgtg catcgactcg
    11881 gcggcgaatt gtcgctgtcg acgcgcctcg tcacgccgtc ggccgaattg ctggcgcttg
    11941 ccggcgccgc gcacgacgcc aatccgcatc gccaggacga gccgtatcgg caggcgctga
    12001 tcggcgtgta cgcgcgcctt gccgccaccg cgcatgaatt gaccgacctt tcgcccgctc
    12061 gcgtcgtaca gacggacgcg gcgcgctacg agacggcgca ggcgtttcgc gccgatctcg
    12121 acacgatcgc cgcctcgctc gcgcagcacg gcgcggcgcc gctggccgcg cgccgcctcg
    12181 aaccgctgcg gcgcgccgtc gacgtgttcg gattccacct tgcggcactc gatctgcggc
    12241 agaacagcga cgtccatcag gcggttgtcg gcgagttgct cgcccgcgcg caggtggcgg
    12301 gcgactattc cgcgctgcag gaggacgagc gcgttgcact cctcgtgcgc gagctcgcga
    12361 cctcccggct gctgcatagc ccgtatctcg actattcgga gcgcgttggc gccgagcttg
    12421 ccgtgctgcg cgtggctgcg ggcattcatg cgcgctacgg cgccgacgcg ttgccgaact
    12481 acgtgatctc gaagtgccaa tcggtttcgg atctgctcga ggtcggtgtg ctgctgaagg
    12541 aggtcgggct tctgcgcgag catgagctcg cgatgaacat cgtgccgctg ttcgaaacga
    12601 tcgacgacct cgaccggtgc ggcgccacga tgcgcgcggc tttcgcgttg ccgctgtacc
    12661 ggacgctcgt tgcgagccgc cgcgactggc aggagatcat gctgggctat tccgacagca
    12721 acaaggacgg cggctacctg acgtcgagct gggcgatcta ccgggcggaa cgcgcgctcg
    12781 tcgatgccac cgcggcgcat gacgtgcggc tgcggctctt ccacggccgc ggcggcaccg
    12841 tcggccgcgg cggtggcccg agctacgacg cgattctcgc gcagccggcg ggcagcgtca
    12901 gcggcggatt gcggctgacc gagcagggcg agatcatcgc gagcaagtat tcggatcccg
    12961 agctcggccg gcgcaatctc gaagc
//
LOCUS       DASSVX010000088         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1269496, whole genome shotgun sequence.
ACCESSION   DASSVX010000088 DASSVX010000000
VERSION     DASSVX010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269496"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFO53_04035"
     CDS             <1..576
                     /locus_tag="VFO53_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HET8875478.1"
                     /translation="ALAALATAAALADGNHKGNGNGNGTGGNDEDNGTTFATKLVGYE
                     ETPVTLNSPGSGSFTAAVNAAGTAIDYTLSYTTLSSAITQSHIHFGRPATTGQIVLFL
                     CTNIGLPAGVTVPMPQACPPGPATITGTLTAADVIARPTQNIDAGAAGFAEMIKAMHA
                     RAAYANVHTTLFTTGEIRGAIEPRDDGNDDD"
     gene            756..1637
                     /locus_tag="VFO53_04040"
     CDS             756..1637
                     /locus_tag="VFO53_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET8875479.1"
                     /translation="MRATLRFLLPLTLLALVGASPVAAQQPVRVGSKNFTEQFVLAEL
                     YAQAMEAAGIKVERKLNLGGTLIAHKALEEKQIDFYPEYTGTMLLAVLKQPAMSERKA
                     VYDKVKTAYAAKGLTLLNEAPVNNTYNMVVRPETAAQYKLVTLSDLAKVANQLKLGAG
                     PEFRDREDGLPGLKAKYGIVFKEDVQMAIGLRYQAIASKQIDVVNGYSTDGMISALKL
                     TRLADDKNLWPPYYVVPVVRNEALAANPKLADVLNRVSATLDESAMAAMNYKVDGEKM
                     EPRDVARDFLKAKGIVH"
     gene            1648..>2394
                     /locus_tag="VFO53_04045"
     CDS             1648..>2394
                     /locus_tag="VFO53_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HET8875480.1"
                     /translation="MVPVCVGERRVRIADVVAVARDDARVELAPVVRARIGAARDVVL
                     RLAQSDTPIYGLNTALGANTGQPLDPDDLAEYQRRAIRARAVAVGPAYDRASVRAMLF
                     ARIAGMAAGGSGVSQPVLDALVEFLNRGIHPVVPRQGSIGVADLPQLSHLALPLIGEG
                     MAEFGGVLLPGAEALRRAGLAGVALGPKDGLAVISANAATIARGALVVADALALVAAW
                     RAAIALSFEGFRANLSPLDPRAVAARPAPGQ"
BASE COUNT          407 a          807 c          816 g          364 t
ORIGIN      
        1 gccctggcgg ccctcgccac cgcagcggcc ctggccgacg gcaatcacaa gggcaacggg
       61 aacgggaatg gcactggcgg caacgacgag gacaacggca cgacgtttgc cacgaagctg
      121 gtcggctacg aggaaacgcc ggtgacgctc aactcgccgg gcagcggctc gttcacggcg
      181 gccgtgaatg ccgccggaac cgcgatcgac tacacgcttt cgtacaccac cctctcgtcc
      241 gcgatcacgc agtcgcatat ccacttcggc cgtcccgcca ccaccggcca gatcgtgctt
      301 ttcctgtgca cgaacatcgg cctgcccgcc ggggtcaccg taccgatgcc gcaggcctgt
      361 ccaccggggc cagcgacgat caccgggacg ctgaccgctg ccgacgtcat tgcgcgacca
      421 acccagaata tcgatgccgg cgccgcgggg ttcgccgaga tgatcaaggc gatgcacgca
      481 cgtgcggcat acgctaacgt tcacacgacg ctattcacga ccggagagat ccgcggcgcg
      541 atcgaaccgc gcgacgacgg caacgacgac gactgatcga aggtttcgtt cgtgggtcct
      601 cgagccgggc acgcctggct ctttttttgt gcgcaaggcc gatcgcgcgc ggcaattgac
      661 cggtccgggc cgcacccgga caatcgcgca ttcgcggatg ccgaacgcga cgggctgcaa
      721 cgtctcggcg acacgcacct tgaaggagca tcgaaatgcg ggccacgctg cgattcctgt
      781 tgccactcac cctgctcgcg ctggttggcg catcgccagt ggccgcacag cagccggtgc
      841 gggtggggtc gaagaatttc accgagcagt tcgtgcttgc cgagctgtac gcgcaggcga
      901 tggaagcggc gggcatcaag gtcgagcgca agctgaacct cggcggcacg ctgatcgcgc
      961 acaaggcgct cgaggaaaag cagatcgact tctaccccga gtacacggga acgatgctgc
     1021 ttgccgtgct gaagcagccg gcgatgagcg agcgcaaggc cgtctacgac aaggtgaaga
     1081 cggcgtatgc ggcgaagggt ctcacgctgc tgaacgaagc gccggtcaac aacacgtaca
     1141 acatggtcgt gcgtcccgag accgccgcgc aatacaagct cgtcacgctg tccgatctgg
     1201 cgaaggtcgc gaaccagctc aagcttggcg ccggccccga gttccgcgat cgcgaggacg
     1261 gcctgccggg cctcaaggcg aagtacggca tcgtcttcaa ggaggacgtg cagatggcga
     1321 tcgggctgcg ctaccaggcc atcgccagca agcagatcga cgtggtcaac gggtattcga
     1381 ccgatggaat gatcagcgcg ctcaagctca cccgtcttgc ggacgacaag aacctgtggc
     1441 cgccgtatta cgtggttccc gtcgtgcgca acgaggcgct cgccgcgaat ccgaagctcg
     1501 ccgacgtgct gaatcgcgtc agcgcgacgc tcgacgaatc ggcgatggct gcgatgaact
     1561 acaaggtcga cggcgagaag atggagccgc gcgacgtcgc gcgcgatttc ctgaaggcga
     1621 agggcatcgt gcattgaggg ggctgacgtg gtgccggttt gcgtcggtga acggcgtgtc
     1681 cgcatcgccg acgtcgtcgc cgtcgcgcgt gacgacgccc gcgtcgagct cgcgccggtc
     1741 gtgcgcgcac gcatcggcgc cgcgcgcgac gtcgtgctcc gccttgcgca gagcgacacg
     1801 ccgatctacg gtctcaacac ggcgctcggg gccaacacgg ggcagccgct cgacccggac
     1861 gatctcgcgg agtaccagcg acgcgcgatc cgcgcccgcg ccgtcgcggt agggccggcc
     1921 tacgaccgcg cgtcggtgcg cgccatgctg ttcgcgcgca ttgcgggcat ggccgccggc
     1981 ggctccggcg tgtcgcagcc ggtcctcgac gcactcgtcg agttcctcaa ccgcgggatt
     2041 catccggtgg tgccgcgtca ggggtcgatc ggcgtggccg acctgccgca actttcgcat
     2101 cttgcactgc cattgatcgg cgagggcatg gccgaattcg gcggcgtctt gctgccgggt
     2161 gccgaagcgc tgcggcgcgc gggactcgcc ggcgtcgcgc tgggtccgaa ggacgggctt
     2221 gcagtgatca gcgcgaacgc ggcgacgatc gcgcgcggcg cgcttgtcgt cgccgacgcg
     2281 ctcgcgctcg tggccgcctg gcgtgcggcc atcgcgctgt cgttcgaggg gtttcgcgcc
     2341 aacctgtcac cgctcgaccc gcgcgcggtt gccgcgcggc cggcgcccgg gcag
//
LOCUS       DASSVX010000089        21006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_952877, whole genome shotgun sequence.
ACCESSION   DASSVX010000089 DASSVX010000000
VERSION     DASSVX010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 21006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21006
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952877"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1616
                     /gene="nuoL"
                     /locus_tag="VFO53_04050"
     CDS             <1..1616
                     /gene="nuoL"
                     /locus_tag="VFO53_04050"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET8875481.1"
                     /translation="WEAVGLVSYLLIGFWYTRPTAIYANLKAFMANRVGDFGFILGIG
                     LVLAYFGTLDYAPVFAKAASMAGTVLPWSGAHWNLMTAICICLFVGAMGKSAQVPLHV
                     WLPDSMEGPTPISALIHAATMVTAGIFMVARMSPLFEWSEAALSFVTIIGAITALFMA
                     FLAIVQNDIKRVVAYSTLSQLGYMTVALGVSAYSAAIFHLMTHAFFKALLFLAAGSVI
                     VALHHEQDLRRMGGLRRYMPITYITAVVGSLALAGIPPFAGFFSKDAIIEAVSLSHIP
                     GHTFAYAAVLGGVFVTAFYSFRMLFLAFHGPERFHGHAEAEHGEHDERAVPSHDPSSE
                     SAEEPDPHGHEGGPPRESPWVITAPLVLLAIPSVYAGWAYIQPMLFGNWFGGSIVVRE
                     PHAVVGELAHEWHGAVPFILHGLVSPTFWLALAGIVTAVYLYLVNPAMPAKIVARFRP
                     IYTLLDNKYYFDRFNDWFFAGGSRWLGRFASNYGDRTIIDGWVVNGSAAFVGFVAREF
                     RRIQSGYVYHYAFVMIVGVFGLLYWWGVR"
     gene            1732..3216
                     /locus_tag="VFO53_04055"
     CDS             1732..3216
                     /locus_tag="VFO53_04055"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET8875482.1"
                     /translation="MSGAMAPYLSLAIWVPIVAGVVVLGFGRDRDAGFARWIALIGAV
                     AGFVVTVPLYLHFDTSTSAMQFAEYADWVPRFDIHYHLGVDGISVLFVLLNSLMTVLV
                     VWAGWEVITTRVAQYMAAFLIMSGLMNGVFVALDGVLFYIFFEAMLIPMYLIIGIWGG
                     PNRVYAALKFFLYTLLGSILMLVALIYLYSVSESFEILEWYKVPLELSVQLFLFVAFF
                     LGFAVKVPMWPVHTWLPDAHVEAPTGGSVVLAAIMLKIGGYGFVRFILPIVPDASRYL
                     SGFIITLSLIAIIYIGFVAIVQADMKKLIAYSSVSHMGFVTLGFFLYSQLGVEGGLVQ
                     MLSHGFVSGAMFLCVGVMYDRLHSRLIADYGGVVNTMPKFAAFMMLFAMANAGLPGTS
                     GFVGEFLVILAAVKVNFWIGFGAALTLILGAAYSLWMYKRVIFGAIANANVAGMIDIG
                     RREFWLLAVFAAAVLWIGIWPAPLVDVMHVSVADLLRVAARSKL"
     gene            3225..4673
                     /gene="nuoN"
                     /locus_tag="VFO53_04060"
     CDS             3225..4673
                     /gene="nuoN"
                     /locus_tag="VFO53_04060"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HET8875483.1"
                     /translation="MFAQLHYWAALPEIVILAAASLILLVDLFVPDDRRHVSYWLTQL
                     TLLLAVCVTLTTLRLDSIKGYHGMVIDDMLADFLRIACFIAVSLMLFYSRSYMIARGL
                     FRGDVFVLTLFALLGMQVMITGNNFLTLYLGLELMSLSLYALVAAQRDHPRGAEAAMK
                     YFVLGALASGMLLYGLSMIYGTTGSLDLDTVARAIYARHESTLLLTFGLVFVVSGLAF
                     KLAVVPYHMWVPDVYDGAPTSMTVLIGTGPKIAAFAMTLRILSGAMQGAAFDWQGMLI
                     VLCVLSMVLGNLIAIAQSNLKRMLAYSTIANMGYMLLGFLTANRYGFSAAMFYTIAYA
                     LTSLASFGMIMLLSREGFESDRLDDFKGLNQRSPWWAFIMLLVMFSLAGLPPTLGFYA
                     KFMVIESAINEGFVWLAVVAVVTSLIGAFYYLRVVKLMYFDEPVESAPIVVRGDTWVA
                     LSANGLLLLVLGILPQQLIGLCAIALTQSNFP"
     gene            4810..5544
                     /locus_tag="VFO53_04065"
     CDS             4810..5544
                     /locus_tag="VFO53_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET8875484.1"
                     /translation="MRVGFFVTCLVDLMRPSIGFAALKLLESGGADVYVPPNQTCCGQ
                     PCYNSGDRADTIALARKVIEEFEDCDYVVAASGSCSGMIRTHYGELFADDLAWSARVA
                     RLAAKTYELTDFLAGVLKVDRVPGDYRGTITYHDSCAGLREMGVKAQPRALLAKMPGV
                     RLSEMADCEVCCGFGGTFAVKLGEISSRIADNKCQHIVTSGADAVVLGDLGCMLNIEG
                     RLRRRGDATKVLHIAEVLAGVMPEPT"
     gene            5555..6976
                     /locus_tag="VFO53_04070"
     CDS             5555..6976
                     /locus_tag="VFO53_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HET8875485.1"
                     /translation="MQVASMYFKERAHTKLHDAQLQASLAKFQGKFVPARRAAITELD
                     DFEGTRDAAAEIRQRALDDLDVWLTIFDENARARGATVLYAQTPAEINALVLEIARRH
                     DVHKIIKSKSMVSEESGLDHAIEAAGLTVVETDLGEYILQINNYEPPSHIIGPALHKS
                     RAEVSDLFAARHGTPRKDAIDELCLEARSVLREHYLTADMGISGGNFFVAETGSVVLV
                     TNEGNATLTTTLPKVHIAISGIEKIVPTLDDVATLMRLLPRSATGQSISNYVDILTGV
                     KGVDEFHGAEHMYFIVVDSGRSAVLGSDVRQALRCIRCGACMNHCPVYQNIGGHAYGW
                     VYPGPIGIVLTPMYVGLEKALDLPYASTMCNQCGVVCPVKIPLPDLQRKLREKEFERN
                     LRPWHERLGLKAWGWLARRPALYGFVSRIGVRLLRALGDRNGMIRTLPLGGGWTDERD
                     MPAPAGRTFRELYKARAAGHAPE"
     gene            6993..8225
                     /locus_tag="VFO53_04075"
     CDS             6993..8225
                     /locus_tag="VFO53_04075"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET8875486.1"
                     /translation="MNAPHPPHVAVPHLSRRGDSLGTENAFVVLAEVNALARSGKDIV
                     SFCIGQPDFPTPRNIQDAAIAAIRDGRHGYTPSAGIDELRAAAARDLGSRRGIDVRPE
                     DVVVGAGAKPFIAYTIASVTDYGVGDEVIYPVPGFPIYESQIVANGAVPVPIFLREAR
                     DFAFDPAELEAKITPKTRLLILNSPQNPTGGILSAADLDAISAILVRHPQIWVFADEI
                     YSRLVYEGRFDSLASRDAMQARTIVCDGASKTWAMTGWRLGYAANRALAPTLTRWITN
                     TESCASHISQWAGVEAIDGPQDSVVEMHARFRERRDLIVRLLNEVPGISCRTPGGAFY
                     AWPNVTEACKRVKAADSEAFRKRLLHDAGIAVLADIHFGRRVPGDGQHIRFSYAASSE
                     AIESGIARLDAFVRKHAM"
     gene            8230..8895
                     /locus_tag="VFO53_04080"
     CDS             8230..8895
                     /locus_tag="VFO53_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HET8875487.1"
                     /translation="MAIDDNQVSREAVLARVRAALHREGPDDEARAAAHAYLAARRQG
                     PRPALPADLVSRFLDRAADMSSTTERVGTIEAVPAAVARYAASLGVASASAVCWPEFA
                     SLDWRAAGVGVDVRPAAGEDAMGITGCFCAIAETGTLVFASSSDTPPATFLLPETHVA
                     IVRGAQIVAGMEDAFARLREERTEMPRALNLVSGPSRTGDIEQTIVLGAHGPRRVHIV
                     LVG"
     gene            complement(8899..10008)
                     /locus_tag="VFO53_04085"
     CDS             complement(8899..10008)
                     /locus_tag="VFO53_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET8875488.1"
                     /translation="MSAVLARPAPATGVVSELAFARAQRNTVLIVDDLFSSRLLLAEI
                     VRQIDGKLNLELFDTPSRALEFARNNRIDIVLTDYKLPEFDGIELVKQIRALPHCVDV
                     PIVVITVVDDRKIRYDALEAGATDFLIKPLDDHETRARCANLLELRRHKIVLSDQARV
                     LQYQVDKSVAEIHERELETLSKLAKAGEFRDKTTGNHLMRMARHSALIGQHLGLGTET
                     VHVLEVAAPMHDIGKIGIPDSVLLKEGPLTPQETQVMREHPRMGYDILKGSPSKYLSM
                     GAIIALGHHEKFDGSGYPNGLHGEDIPLVGRIVAVADVFDALVSERPYKHAWSLDDAI
                     EFLRSQRGKHFDPTCIDAFLSDRSAIETVMREFAD"
     gene            10359..11210
                     /locus_tag="VFO53_04090"
     CDS             10359..11210
                     /locus_tag="VFO53_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8875489.1"
                     /translation="MVQAALQKTSLDAGPRRAGGDALAAEARFAADLGGRIRTLRDRR
                     GMSRKLLARTSNVSERYLAQIESGNGNISVMLLRRVAHALGVAIGDVLEPGDEAGAVP
                     RRRIALIGMRGAGKSTLGAMLAEALHLPLTELRHEVTRETGLPAGEVIALYGHAAYRR
                     IERRVLARVAREQAQAVIVAGGGIVDEEDAFDLLRANCYTAWIKARPEEHMARVLAQG
                     DLRPMAGNARAMDDLKRILAARDAMYRRADVTIDTSGETAQQSFAKLRAAVASAFAPA
                     TLQMESA"
     gene            11207..12874
                     /gene="boxC"
                     /locus_tag="VFO53_04095"
     CDS             11207..12874
                     /gene="boxC"
                     /locus_tag="VFO53_04095"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HET8875490.1"
                     /translation="MSAAREPPRVDYQTQPDRYRHYRVRYDGAVATLALDVDENGGIR
                     PGYALKLNSYDLGVDIELHDALQRIRFEHPEVRAVVLTSGRERVFCSGANIFMLGRSS
                     HAWKVNFCKFTNETRNAIEDASRHSGLKFLAACNGTTAGGGYELALACDEIVLVDDRS
                     SAVSLPEVPLLGVLPGTGGLTRLTDKRRVRHDLADIFCTTTEGIRGTRAKEWRLVDEV
                     VKPSEFAAYVARRAGELAGISDRPATARGVALSPLARTIDDTGYRYEFVDVRIDRAAR
                     RATLCVKAPSADQPDDVDEIEHAGARWWPLQMARELDDAILMLRTNDLDIGIWILTTD
                     GDIDRVLAVDDTLARHAGHWFVRETIGLLRRTFARLDVSSRTLIALVERGSCFAGTLF
                     ELALAADRTYMLDLPDEPQAAPKIAVSAMNFAAYPRVDGQPRIGARFYAEAQPIAEVR
                     AVQGSKLGASDAVRLGLVSVALDELDWSDEIRLVLEERASMSPDALSGMEANLRFGGC
                     ETMHTRVFGRLSAWQNWIFVRPNAIGEAGALKVFGTGTKARFDWDRV"
     gene            12911..14341
                     /gene="boxB"
                     /locus_tag="VFO53_04100"
     CDS             12911..14341
                     /gene="boxB"
                     /locus_tag="VFO53_04100"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HET8875491.1"
                     /translation="MQTSMNYGDRIPNNVNLSDDRALQRALERWQPAFLDWWRDMGPD
                     GSSNFDVYLRTAVSVEPQGWAEFGYVKMPDYRWGIFLTPVAGGASIGFGEHKGAPVWH
                     DVPGEHRANLRRIVVTQGDTEPASVEQQRHLGLTCPSLYDLRNLFQVNVEEARHLWAM
                     VYLLHRYFGRDGREEAEALLTRRSGDADNPRILGAFNERTPDWLSLFMFTYFTDRDGK
                     FQLAALAESGFEPLARTTRFMLTEEAHHMFVGESGVARVIQRTCDVMKQMQIGDPADV
                     RAQGVVDLDTIQRYLNFHYSVTIDLFGADQSSNAATFYTAGLKGRYEEAKRDDDHVLK
                     GRSYPVLDARDGRLVDRDVPMLNALNEVLRDDFIRDSVAGIGRWNRVIEKAGIAFRLQ
                     VPHKAFHRKIGSLAQLRVAPDGSVVSDTEWDANVHRWLPTADDRAFVAALMGRVVEPG
                     RFANWIAPPAAGVNRQPASFEYVRFN"
     gene            14371..15597
                     /gene="boxA"
                     /locus_tag="VFO53_04105"
     CDS             14371..15597
                     /gene="boxA"
                     /locus_tag="VFO53_04105"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxA"
                     /protein_id="HET8875492.1"
                     /translation="MRAASSPDYALQHLIDPEVCIRCNTCEATCPIGAVTHDARNYVV
                     DFAKCNACNACLPPCPTGAIDHWQAVRKSAPYTLADQYDWDSLPAPVAVEAGHAGEIP
                     AEVRQLAEVASAGQAGATSAPWSAAQPCVNLYLPAQPAIARVTGNYRLTAVDASADIR
                     HIVLDFGSTAFPVLEGQTLGILTPGEDAHGKPHYVRLYSVASPRDGERPGHNNIALTV
                     KRVTVDHDGRAVAGVASNYLCDLALGDEVKVVGPYGTSYLMPDHPGSSLMMICTGTGS
                     APMRAMTERRRRRRMLKEGGELLLFFGARVEHELPYFGPLTKLPCEFIDVNFAFSRVS
                     GQPKVYVQDRIRERAASVARMLQDDDCYIYVCGLRGMEKGVIDAFRDVCDTHGVEWNA
                     LLPALQSTGRLHIETY"
     gene            complement(15622..15783)
                     /locus_tag="VFO53_04110"
     CDS             complement(15622..15783)
                     /locus_tag="VFO53_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875493.1"
                     /translation="MGQTLVHPVRMSQLEWRRTTSADDALDAMRGILCAILVSMLGFW
                     IPLAVALTP"
     gene            16025..17011
                     /locus_tag="VFO53_04115"
     CDS             16025..17011
                     /locus_tag="VFO53_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875494.1"
                     /translation="MRKALAVLAILLCSATPSLAQVSLGFNSPGVSIGVNLPVYPDLV
                     RIPGYPVYYAPQVNANYFFYDGLYWVFDGVNWYASTWYNGPWQLVTSDAVPLFLWRVP
                     VRYYRERPAYFRGWALNAPPRWGAHWGNTWAQRHRGWDRWNRASVPAAAPLPTYQRRY
                     AGNRYPRGDEQRALETRNYRYQPRERVAQQHFQQRSTQSTPAARPQGRTGFAENGRER
                     GRERNGIDRTQNANRNVQRVAPPATAQRGGPPVTAQRAAPRERGAPPTARRAGPPDVE
                     NGAPRAAPPRGEARAAQGRGPQAREPAQAANRRGNSGAAHARGDERRDEHNG"
     gene            17198..18277
                     /gene="ada"
                     /locus_tag="VFO53_04120"
     CDS             17198..18277
                     /gene="ada"
                     /locus_tag="VFO53_04120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HET8875495.1"
                     /translation="MTTQAINDTAAARTQSDPRWAAVVARDARADGTFFYSVRTTGVY
                     CRPSCAARVARPENVAFHASTHDAERAGFRPCKRCRPDRLSPSQAQAALVSTLCRFIE
                     AADHVPTLAELATRAGLSAHHLHRTFRAITGVTPRAYAAAHRARRVRDALAASTTVTD
                     AIYDAGYGSNGRFYDEANGVLGMTPTRFRAGGAGTRIHFAIGECSLGSILVAQSERGV
                     CAIFLGDDPDRLARDLQDRFPNAQLVGGDATFEGVVAKVVGFVESPDARLDLPLDVRG
                     TAFQRRVWQAMREIAPGETASYSELARRIGAPKSTRAVAAACAANVLAVAIPCHRVVR
                     SDGALSGYRWGVERKRALIEREARK"
     gene            18291..19040
                     /locus_tag="VFO53_04125"
     CDS             18291..19040
                     /locus_tag="VFO53_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET8875496.1"
                     /translation="MNARANDEAVRAAAGGIEPRIDAVDWARVAVELDAQGSSVIERL
                     LTPGECASLVSLYGSDAAFRGRVVMARHGFGRGEYKYFAYPLPAVVANLRSALYSRLA
                     PIANRWYEAMRIDVRFPARHDEFLERCHAAGQRRPTPLLLQYGADDYNCLHQDLYGAQ
                     VFPLQITILLSEPGRDFDGGEFVMTEQRPRMQSRPHVVPLRQADAVVFAVHQRPVRGA
                     RGFYRVNLRHGVSRVRTGHRHTAGIIFHDAA"
     gene            19037..19690
                     /gene="alkB"
                     /locus_tag="VFO53_04130"
     CDS             19037..19690
                     /gene="alkB"
                     /locus_tag="VFO53_04130"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HET8875497.1"
                     /translation="MRTRDLFDTAARGPYVETLAPGAAILRGFARPFEDALIDALNDV
                     VASAPLRHMVTPGGFRMSVAMTNCGSLGWVSSATGYRYDAVDPDNGNRWPRMPRAFLR
                     LAKDAAARAGFAGFVPDACLVNRYAPGARLTLHRDRNERDFEAPIVSVSLGLPATFLF
                     GGLARSDRPLRVQLHHGDTVVWGGPARLRYHGVAALEAGEHDRMGAQRINLTLRKAG"
     gene            complement(19703..20308)
                     /locus_tag="VFO53_04135"
     CDS             complement(19703..20308)
                     /locus_tag="VFO53_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HET8875498.1"
                     /translation="MKRVCLIVIALLVGVSAGCADMSPQSPATGWVTLLDGPKGFTEN
                     WDRIGDANWRIVDGVAQADTGEKGKSSFLMTKNAYGDFMLRAEFWVSDDANSGIYMRC
                     PDLRDIKDTNCTEANIFDQRPDPTYGTGAITHLSAITNMPKAGGKWNTYEITARGPRI
                     VVVMNGVQTGETDKANALRGNIGLQWAAGVVKFRKVEIKPL"
     gene            20480..>21006
                     /locus_tag="VFO53_04140"
     CDS             20480..>21006
                     /locus_tag="VFO53_04140"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET8875499.1"
                     /translation="MDNVTHTLIGALLGESAARLAPPAGNGRQAKLRRGLFLAVAIVG
                     SNFPDADLLSTLGADGKLAYLLEHRGHTHTVAGALLISLAMLGGCALWQRWRGLVLPA
                     RDRRWLVALALLAPLFHIAMDALNTYGVHPWWPFDNRWYYGDAVFIVEPLFWAAAAPL
                     AFLLRSCTARAFVVLA"
BASE COUNT         3241 a         6920 c         7110 g         3735 t
ORIGIN      
        1 gctgggaagc ggtcggcctc gtatcgtatc tgctgatcgg cttctggtac acgcggccga
       61 cggcgatcta cgcaaatctc aaggccttca tggccaaccg cgtcggcgac ttcggcttca
      121 tcctcgggat cgggctcgtg ctcgcgtact tcggcacgct cgactacgcg ccggtattcg
      181 cgaaggcggc gtcgatggcc ggtaccgtgc tgccctggag cggggcgcac tggaacctga
      241 tgacggccat ctgcatctgc ctgttcgtcg gcgccatggg caagagcgcg caggtgccgc
      301 tgcacgtgtg gctccccgat tcgatggagg gcccgacgcc gatctcggcg ctgatccacg
      361 cggccacgat ggtcaccgcc ggcatcttca tggtcgcgcg gatgagcccg ctgttcgaat
      421 ggtcggaagc ggcgctgtcg ttcgtcacga tcatcggcgc gatcaccgcg ctgttcatgg
      481 cgtttctcgc gatcgtgcag aacgacatca agcgggtcgt cgcgtattcg acgctgtcgc
      541 agctcggcta catgacggtg gcgctcggcg tgtccgccta ttcggcggcg atcttccacc
      601 tgatgacgca cgcgtttttc aaggcgctgc tgttcctcgc ggcggggtcg gtgatcgtcg
      661 cgcttcatca cgagcaggac ctgcgccgga tgggcggcct gcgcaggtac atgccgatca
      721 cgtacatcac cgcggtcgtc gggtcgctgg cgcttgcggg aattccgccg tttgccggct
      781 tcttctcgaa ggacgcgatc atcgaggcgg tatcgctgtc gcacattccc ggccacacgt
      841 ttgcgtacgc cgcggtgctc ggcggcgtat tcgtgaccgc gttctactcg ttccggatgc
      901 tgttcctcgc attccacgga ccggagcgtt tccacgggca cgcggaagcc gagcacggcg
      961 agcacgacga gcgcgcggtc ccgtcgcacg acccgtcgtc ggaaagcgcc gaggagcccg
     1021 acccgcacgg ccacgagggc ggaccgccgc gcgaatcgcc gtgggtgatc accgcgccgc
     1081 tggtgctgct cgccatcccg tcggtctacg ccggctgggc gtacatccag ccgatgctgt
     1141 tcggcaactg gttcggcggc tcgatcgtcg tgcgcgagcc gcatgcggtc gtcggcgagc
     1201 tcgcgcacga gtggcacggc gccgtgccgt tcatcctgca cggcctcgtg tcgccgacgt
     1261 tctggctcgc gctcgcgggt atcgtcacgg ccgtctacct gtacctggtg aatccggcga
     1321 tgcccgcgaa gatcgtcgcg cgcttccgtc ccatctacac gctgctcgac aacaagtact
     1381 acttcgaccg cttcaacgac tggttcttcg ccggcgggtc gcgctggctc ggccggtttg
     1441 catcgaacta cggtgaccgg acaatcatcg acggatgggt ggtcaacggc tcggctgcgt
     1501 tcgtcggctt cgtcgcgcgc gaattccggc gcatccagtc gggctatgtc tatcactatg
     1561 cgttcgtgat gatcgtcggc gtcttcgggc tgctctactg gtggggcgtg cgatgagtcc
     1621 gtcgggcgag cgacagcgag ccaaggcccg aaggggccgc cgacggacga gtcgcacgag
     1681 gccgacactc gcagcttcgg ttcgcgccgc tggtacagcg aggccgaggc gatgagcgga
     1741 gcgatggcgc cgtatctgtc gctcgcgatc tgggtgccga tcgtcgccgg cgtcgtcgtg
     1801 ctcggcttcg gccgcgaccg cgatgcaggc ttcgcgcgct ggatcgcgct gatcggcgcg
     1861 gttgccgggt tcgtcgtcac ggtgccgctg tatcttcatt tcgacacatc cacgtcggcg
     1921 atgcagttcg ccgaatacgc agactgggtg ccgcgcttcg acatccatta tcacctcggc
     1981 gtcgacggca tctcggtcct gttcgtcctt ctcaacagcc tcatgaccgt gctcgtcgtc
     2041 tgggccggct gggaagtgat cacgacgcgc gtcgcgcagt acatggccgc gttcctgatc
     2101 atgtcgggtc tcatgaacgg cgtgttcgtc gcgctcgacg gcgtgctttt ctacattttc
     2161 ttcgaggcga tgctgatccc gatgtacctg atcatcggca tctggggcgg ccccaaccgc
     2221 gtctacgcgg cgctcaagtt ctttctctac acgctgctcg gctcgatcct gatgctggtc
     2281 gcgctgatct acctctacag cgtgtccgag agcttcgaga tactcgagtg gtacaaggtg
     2341 ccgctcgaat tgagcgtgca gttgttcctg ttcgtcgcgt tcttcctggg cttcgccgtc
     2401 aaggttccga tgtggcctgt gcatacgtgg ctgccggatg cgcacgtcga ggcgccgacc
     2461 ggcggctcgg tcgtgctggc ggcgatcatg ctcaagatcg gcggttacgg cttcgtgcgc
     2521 ttcatcctgc cgatcgtccc ggacgcgtcg cgctacctgt ccgggttcat catcacgctg
     2581 tcgctgatcg ccatcatcta catcggcttc gtggccatcg tgcaggccga catgaagaag
     2641 ctgatcgcgt attcgtcggt atcccacatg ggattcgtga cgctgggctt ctttctctac
     2701 agccagctcg gcgtcgaagg cgggctggtg cagatgctgt cgcacgggtt cgtgtccggg
     2761 gcgatgttcc tgtgcgtggg cgtcatgtac gaccggctgc attcgcgttt gatcgccgac
     2821 tacggtggcg tcgtcaacac gatgccgaaa ttcgccgcat tcatgatgct gttcgcaatg
     2881 gcgaacgccg gcctgcccgg cacgtcgggc ttcgtcggcg agtttctcgt gatcctcgcg
     2941 gcggtgaagg tgaatttctg gatcggcttc ggcgcggcgc tcacgctgat cctcggcgcc
     3001 gcgtactcgc tgtggatgta caagcgcgtg atcttcgggg cgattgcgaa cgccaacgtc
     3061 gccgggatga tcgacatcgg ccgccgcgaa ttctggctgc ttgcggtctt tgcggccgcg
     3121 gtcctctgga tcggcatctg gcccgcgcct ctcgtcgacg tcatgcacgt ctcggtcgcc
     3181 gacctgctgc gcgttgccgc gcgctcgaaa ctgtaggcgg ggccatgttc gctcaactcc
     3241 attactgggc cgcgctcccc gagatcgtca tcctcgccgc ggcgtcactg atcctgctgg
     3301 tcgacctctt cgtgcccgac gaccggcggc acgtcagcta ctggctgacg cagctcacgc
     3361 tgctgctcgc ggtctgcgtc acgctgacca cgctgcgcct cgactcgatc aagggctatc
     3421 acggcatggt catcgacgac atgctcgccg atttcctgcg catcgcctgc ttcatcgccg
     3481 tgtcgctgat gctgttctac tcgcgcagct acatgatcgc gcgcgggctc ttccgcggcg
     3541 atgtcttcgt gctgacgctg ttcgcgctgc tcggcatgca ggtgatgatc accggcaaca
     3601 acttcctgac gctctatctc ggactcgagc tcatgtcgct gtcgctgtac gcgctcgtcg
     3661 cggcgcagcg cgaccacccg cgaggcgccg aggccgccat gaagtacttc gtgctcggcg
     3721 cgctcgcgtc ggggatgctg ctctacggcc tgtcgatgat ctacggcacc acgggatccc
     3781 tcgatctcga tacggttgcg cgcgccatct atgcccggca cgaaagcacg ctgctgctga
     3841 cgttcgggct cgtgttcgtc gtgtcgggcc tcgcgttcaa gcttgccgtc gtgccgtatc
     3901 acatgtgggt acccgacgtg tacgacggcg cgccaacgtc gatgacggtg ctgatcggca
     3961 ccggcccgaa gatcgccgcg ttcgcgatga cgctgcgcat cctctccggc gcgatgcagg
     4021 gcgcagcgtt cgactggcag ggcatgctga tcgtgctgtg cgtgctgtcg atggtcctcg
     4081 gcaacctgat cgccatcgcc cagtcgaatc tgaagcgcat gctcgcctac tcgacgatcg
     4141 cgaacatggg ttacatgctg ctcgggttcc tgacggcgaa ccggtacggc ttttcggcgg
     4201 cgatgttcta cacgatcgcg tatgcgctga cgagcctcgc ctcgttcggc atgatcatgc
     4261 tgctgtcgcg cgagggcttc gagagcgacc ggctcgacga tttcaagggg ctgaaccagc
     4321 gctcgccgtg gtgggcgttc atcatgctgc tcgtgatgtt ctcgctcgcc ggcctgccgc
     4381 cgacgctcgg cttctacgcg aagttcatgg tgatcgagtc ggcgatcaac gaaggcttcg
     4441 tgtggcttgc ggtggtcgcg gtcgtcacgt cgctgatcgg cgccttctat tacctgcgcg
     4501 tcgtcaagct gatgtatttc gacgagccgg tcgaaagcgc accgatcgtc gtgcgcggtg
     4561 acacgtgggt cgcgctgtcg gcgaacgggc tgctgctgct cgtgctcggc atcctgccgc
     4621 aacagctgat cgggctttgc gctatcgcgc tgacgcagtc caatttcccc taggatggac
     4681 accgccccgc gcgctccgct cgctgccccg cggggggctg gtcggtcggt cggggcggcc
     4741 gttcccggct gacggcgcgg ggcaaggcgc gcgctcacgc ctcatgcgcg tgctaccatc
     4801 cgcgctccca tgcgcgtcgg cttcttcgtc acctgcctcg tcgacctgat gcggccctcg
     4861 atcgggttcg cggcgctgaa gctgctggaa tccggtggcg ccgacgttta tgtgccgccg
     4921 aatcagacgt gctgcgggca gccgtgctac aactcgggcg atcgcgccga tacgatcgcg
     4981 ctggcgcgca aggtgatcga ggagttcgag gactgcgact acgtcgttgc ggcgtcgggc
     5041 tcgtgcagcg gcatgatccg cacgcactac ggcgagctct tcgccgacga cctcgcgtgg
     5101 tcggctcgcg tcgcgcggct cgcggcgaag acgtacgagc tcaccgactt cctcgccggc
     5161 gtgctgaagg tcgaccgcgt gccgggcgac tatcgcggca cgatcaccta tcatgattcg
     5221 tgtgcgggat tgcgcgagat gggcgtcaag gcgcagccgc gcgcgctcct cgcgaagatg
     5281 cccggcgtcc gcctctcgga gatggccgat tgcgaggtgt gctgcggctt cggcggcacg
     5341 ttcgccgtga agctcggcga aatctcgtcg cgcatcgccg acaacaagtg ccagcatatc
     5401 gtgaccagcg gcgccgacgc ggtcgtgctg ggcgatctcg gctgcatgct gaacatcgag
     5461 gggcggctgc ggcgccgggg cgacgcgaca aaggtgctgc acatcgccga agtgctggcc
     5521 ggtgtgatgc ccgagccgac ctgacgagcc gccgatgcag gtcgcatcga tgtacttcaa
     5581 ggagcgtgcg cacacgaagc tgcacgatgc gcaactgcag gcgagcctcg ccaagttcca
     5641 ggggaagttc gttccggccc gcagggcggc gatcaccgag ctcgatgact tcgaaggcac
     5701 gcgcgacgcc gcggcggaga tccgccagcg cgcgctcgac gacctcgacg tctggctcac
     5761 gatcttcgac gagaacgcgc gtgcgcgcgg cgcgacggtg ctctacgcgc agacaccggc
     5821 ggagatcaac gcgctcgtgc tggagatcgc gcgccgccac gacgtgcaca agatcatcaa
     5881 gtcgaagtcg atggtgtcgg aggagtccgg gctcgatcat gcgatcgagg cggcgggcct
     5941 cacggtggtc gagaccgacc tcggcgaata catcctgcag atcaacaatt acgagccgcc
     6001 gtcgcacatc atcgggccgg cgctgcacaa gtcgcgcgcc gaggtgtccg accttttcgc
     6061 ggcgcgtcac ggcacgccgc gcaaggacgc gatcgacgag ctgtgcctcg aggcgcgcag
     6121 cgtgctgcgc gagcattacc tcaccgccga catgggcatc tccggcggca atttcttcgt
     6181 cgccgaaacg ggctcggtcg tcctcgtgac gaacgagggc aacgcaacgc tcaccacgac
     6241 gctgccgaag gttcacatcg cgatctcggg catcgagaag atcgttccga cgctcgacga
     6301 cgtggcgacg ctgatgcgcc ttctgccgcg ctcggcgacc gggcagtcca tttcgaatta
     6361 cgtcgatatc ctgacgggcg tcaagggagt ggacgagttc catggcgccg agcacatgta
     6421 tttcatcgtc gtcgacagcg gtcgcagtgc ggtgctcggc agcgacgtgc gccaggcatt
     6481 gcgctgcatt cgttgcggcg catgcatgaa ccactgtccg gtctatcaga acatcggcgg
     6541 ccacgcctac ggctgggtct atccgggccc gatcggcatc gtgctgacgc cgatgtacgt
     6601 ggggctcgag aaggcgctcg acctgccgta tgcgtcgacg atgtgcaatc agtgcggtgt
     6661 cgtgtgtccg gtaaagatcc cgctgcccga cctgcagcgc aagcttcgcg aaaaggaatt
     6721 cgagcggaac ctgcgcccct ggcacgagcg gctcggattg aaggcgtggg gttggctcgc
     6781 gcggcggccg gcgctctatg ggttcgtttc gcgcatcggc gtgcgtctgc tgcgcgcatt
     6841 gggcgatcgc aacggcatga tccgcacgct gccgctgggc ggcggctgga ccgacgagcg
     6901 cgacatgcca gcgcccgcgg gacgcacgtt ccgcgagctc tacaaggcac gcgctgccgg
     6961 gcacgcaccg gagtaacgga aggaatcatc ggatgaatgc gccacacccg ccgcatgtcg
     7021 cggtcccgca tctgtcgcgc cgcggcgatt cgctcggcac cgagaatgcg ttcgtcgtgc
     7081 tcgccgaagt gaatgcgctc gcgcggagcg gcaaggacat cgtgtcgttc tgcatcggcc
     7141 aacccgactt cccgacgccg cgcaacatcc aggatgcggc gatcgccgcg attcgcgacg
     7201 gcaggcacgg atacacgccg tcggccggca tcgacgaact gcgcgccgcc gcggcgcgcg
     7261 atctcggcag ccggcgcggc atcgacgtgc gtcccgaaga cgtcgtggtc ggcgctggcg
     7321 cgaagccgtt catcgcgtac acgatcgcgt cggtcaccga ttacggcgtg ggcgacgagg
     7381 tcatctatcc ggtgccggga tttccgatct acgagtcgca gatcgtcgcc aacggcgcgg
     7441 tgccggtgcc gatcttcctg cgcgaggcgc gcgatttcgc gttcgatcct gccgagctcg
     7501 aggcgaagat cacgccgaag acgcggcttc tgattctcaa ttcgccgcag aacccgaccg
     7561 ggggaattct ttcggcggcc gacctcgacg cgatctccgc gatcctcgtt cggcatccgc
     7621 agatatgggt attcgccgac gagatctact cgcggctcgt ctacgaaggc cgcttcgaca
     7681 gcctggcgag ccgcgacgcg atgcaggcgc gcacgatcgt ctgcgacggc gcgtcgaaga
     7741 cctgggcgat gacgggatgg cggctcggct acgcggccaa ccgcgcgctc gcgccgacgt
     7801 tgacgcgctg gatcaccaac accgaatcgt gcgcttcgca catctcgcaa tgggccggcg
     7861 tcgaggccat cgacgggcca caggattcgg tcgtcgagat gcatgcgcgc tttcgcgagc
     7921 gccgcgacct catcgtgcgg ctgctgaacg aggtgccggg catctcgtgc aggacgccgg
     7981 gcggcgcgtt ctacgcgtgg ccgaatgtca ccgaagcgtg caagcgcgtg aaggcggccg
     8041 attcggaagc gttccgcaag cgcctgttgc acgatgcggg gatcgccgtg ctcgctgaca
     8101 ttcacttcgg ccgtcgcgtt ccgggggatg gacagcacat ccgcttttcg tatgcggcat
     8161 cgagcgaggc gatcgaaagc ggcatcgcgc ggctcgatgc gttcgtgcgc aagcacgcga
     8221 tgtagcgcga tggcgatcga cgacaaccag gtgtcgcgcg aggcggtgct cgcgcgcgtg
     8281 cgcgccgcgc tgcaccgcga agggcccgac gacgaggcgc gggcggccgc ccacgcctat
     8341 ctcgccgccc gccggcaagg tccgcgaccg gcgcttcccg ccgatctcgt ctcgcgcttc
     8401 ctcgatcgcg ccgccgatat gtcgagcacg accgagcgcg tcggcacgat cgaagcggtg
     8461 ccggcggcgg tggcgcgcta tgccgcgagc ctcggcgtgg cgagcgcgag cgccgtgtgc
     8521 tggcccgagt ttgcatcgct cgactggcga gcggccggtg tcggtgtcga cgtgcggccc
     8581 gccgccggcg aggatgcaat gggcatcacc ggctgctttt gcgcgattgc cgaaaccggg
     8641 acgctggtat tcgcaagcag cagcgacacg ccccccgcga ccttcctttt gcccgagacg
     8701 cacgttgcga tcgtgcgtgg cgcgcagatc gtggcgggga tggaggacgc gtttgcgcgg
     8761 ttacgcgagg aacgaacgga aatgccgcgt gcgctgaacc tcgtgtcggg accctcgcgc
     8821 accggcgaca tcgagcagac gatcgtgctc ggcgcgcatg gtccgcgccg cgtgcatatc
     8881 gtgctggtcg gctgagactt aatccgcgaa ttcgcgcatc accgtctcga tcgccgagcg
     8941 atcggacagg aacgcgtcga tgcacgtcgg atcgaagtgc ttgccgcgct gtgacctgag
     9001 gaactcaatc gcatcgtcga gcgaccacgc gtgcttgtac ggacgttccg acacgagcgc
     9061 atcgaacacg tcggccacgg cgacgatgcg cccgacgagc ggaatgtcct cgccgtgcag
     9121 cccgttcgga tagcccgaac cgtcgaactt ctcgtggtgc ccgagggcga tgatcgcgcc
     9181 catcgacagg tatttcgacg ggctgccctt caggatgtcg tagcccatcc gcgggtgctc
     9241 gcgcatgacc tgcgtctcct gcggagtcag cggaccctcc ttcagcagca ccgaatcggg
     9301 aatgccgatc ttgccgatgt cgtgcatcgg cgcagccacc tcgagcacat gcaccgtttc
     9361 cgtcccgagg ccgaggtgct ggccgatcag cgccgaatgc cttgccatgc gcatcaggtg
     9421 gttgccggtg gtcttgtcgc ggaattcgcc cgccttcgcg agcttggaca gcgtctcgag
     9481 ctcgcgctcg tggatctcgg cgaccgactt gtcgacctgg tattgcagca cgcgcgcctg
     9541 gtccgacagc acgatcttgt gccggcgcag ctcgagaagg ttcgcgcacc gcgcgcgcgt
     9601 ctcatgatcg tcgagcggct tgatcagaaa gtcggtggcg ccggcctcga gcgcgtcata
     9661 gcggatcttg cgatcgtcga cgacggtgat cacgacgatc ggcacgtcga cgcagtgggg
     9721 cagcgcgcgg atctgcttga cgagctcgat gccgtcgaac tccggcagct tgtagtcggt
     9781 gaggacgatg tcgatgcgat tgttacgcgc gaactcgagc gcgcgcgacg gcgtatcgaa
     9841 gagctccagg ttcagcttgc cgtcgatctg gcggacgatt tccgcgagca gcagccggct
     9901 gctgaacagg tcgtcgacga tcagcacggt gttgcgctgc gcacgcgcga acgcaagctc
     9961 cgagacaacg ccggtggcgg gtgcgggacg tgccagaacg gccgacatgg gttccttttt
    10021 gggagggatg cgtccgttta acgggacacg aacacgaatg ggcgcaacat tagcaagaac
    10081 agtgcccgca actttgctgc gcaacccacc cctacgcttc ccgaaattca ccgcgaccac
    10141 cttgtcgaat cagtcattta acatcgaacc gtccagaagg tgccgcaagc gcgcaagggc
    10201 agccgggacg tccttgccgc cttgcgtcgc gaatgtgacg gccactttcg ccatcgtgcc
    10261 acggcgaccc cgggatcgcc agttttacga aatcgcgcga ccttccactg caatataatg
    10321 cctggtagtc tttatggctt gcggtagtat aatgccgcat ggtccaggct gcactgcaga
    10381 agacttcgct cgacgcgggc cctcgtcgtg cgggcgggga tgctttggca gccgaggcgc
    10441 gctttgccgc cgatctcggc gggcgcattc gcacgttgcg cgatcgccgg ggcatgtcgc
    10501 gcaagctgct cgcgcgcaca tcgaacgtgt ccgagcgtta tctcgcgcaa atcgagtccg
    10561 gcaacggcaa catctcggtg atgctgctgc gtcgcgtcgc gcatgcactc ggcgtggcga
    10621 tcggcgacgt tctcgagccc ggcgacgaag cgggcgccgt gccgcgcagg cgcattgcac
    10681 tgatcggcat gcgtggcgca gggaagtcga cgctcggcgc aatgctcgcc gaggcgttgc
    10741 accttccgtt gaccgaattg agacacgaag tgacacgcga gaccgggctg cccgcaggcg
    10801 aggtgattgc actgtatggt catgccgcgt atcggcgtat cgaacggcgt gtgctcgcac
    10861 gcgtcgcgcg cgagcaggcg caggcggtga tcgtcgccgg cggcggcatc gtcgacgagg
    10921 aagacgcgtt cgatcttctt cgcgcgaact gctacaccgc atggatcaag gcgcggcccg
    10981 aggagcacat ggcgcgcgtg ctcgcgcagg gcgacctgcg gccgatggcg ggcaatgcgc
    11041 gcgcaatgga cgacctgaag cggattcttg ccgcgcgcga cgcgatgtac cggcgcgccg
    11101 atgtcacgat cgacacgtcg ggagagacgg cgcagcaaag cttcgccaag ctgcgcgcgg
    11161 cggtcgcatc cgccttcgcg ccggcaacgc tgcagatgga gagcgcatga gtgccgcccg
    11221 cgagccgcca cgcgtcgact accagacgca gcccgaccgc taccgccatt atcgtgtgcg
    11281 ctacgacggc gcggttgcga cgcttgcgct cgacgtcgac gagaacggcg gcatccggcc
    11341 cgggtacgcg ctgaaactca actcgtacga ccttggcgtc gacatcgagc tccacgacgc
    11401 gctgcagcgc atccgcttcg agcatccaga ggtgcgcgcg gtcgtgctca cgagcggccg
    11461 ggaacgcgtg ttctgctcgg gcgccaacat cttcatgctc ggccgctcgt cgcatgcctg
    11521 gaaggtgaac ttctgcaagt tcactaatga aacgcgcaat gcgatcgagg acgcgagccg
    11581 gcactcgggc ctcaagttcc tcgcggcctg caatggcacg acggccggcg gcggttacga
    11641 gcttgcactc gcctgcgacg aaatcgtgct cgtcgacgat cgctcgtccg cggtcagcct
    11701 gcccgaagtg ccgctcctcg gcgtgttgcc gggcaccggt ggcctcacgc gcctgaccga
    11761 caagcgcagg gtgcgccacg acctcgccga catcttctgc acgacaaccg aaggcatccg
    11821 cggcacgcgg gcaaaggagt ggcggctcgt cgacgaggtc gtcaagccca gcgagttcgc
    11881 ggcgtacgtc gcgcgtcgcg caggcgagct cgccggcatc agcgaccggc cggccacggc
    11941 ccgcggcgtc gcgctttcgc cgcttgcaag aacgatcgac gacaccggct atcgctacga
    12001 attcgtcgac gtccgcatcg atcgcgcggc gcggcgtgca acgctttgcg tcaaggcgcc
    12061 gtcggccgat caacccgatg acgtcgacga aatcgagcac gcgggcgcgc gctggtggcc
    12121 gctgcagatg gcgcgggagc tcgacgacgc aatcctgatg ctgcgcacga acgatctcga
    12181 catcgggatc tggatcctta ccaccgacgg cgacatcgat cgcgtcctgg cggtcgacga
    12241 cacgctcgca agacacgccg ggcactggtt cgtgcgcgag acgatcgggc tgctgcgtcg
    12301 cacgttcgcg cgcctcgacg tgtcgtcgcg cacgctgatc gcactcgtcg agcgcggctc
    12361 gtgctttgcc ggtacgctgt tcgagctggc gctcgccgcc gatcgcacgt acatgctcga
    12421 cctgcccgac gaaccgcaag ccgcgccgaa gatcgcagtg tcggcgatga atttcgccgc
    12481 gtatccgcgt gtcgacggcc agccgcgaat cggcgcgcgc ttctatgccg aagcgcagcc
    12541 gattgccgag gtgcgggcgg tgcaggggtc gaagctcggg gcgagcgacg cggtgcggct
    12601 cggtctcgtc tcggtggccc tcgacgagct cgactggagc gacgaaatcc ggctggtgct
    12661 cgaagagcgc gcgagcatgt cgcccgatgc gctgagcggc atggaagcga accttcgctt
    12721 cggtggctgc gagacgatgc acacgcgcgt cttcggccgc ctctccgcat ggcagaactg
    12781 gatcttcgtg cggcccaacg cgatcggcga agcgggcgcc ttgaaggtgt tcgggaccgg
    12841 caccaaggcg cggttcgact gggatcgcgt atagcggggc gcgctcgcgc ccattcatgc
    12901 ggagaagtcg atgcaaacgt cgatgaatta cggcgaccgg attccgaaca acgtgaacct
    12961 gtccgacgat cgcgcgctgc agcgtgcgct cgagcgctgg cagccggcat tcctcgactg
    13021 gtggcgcgac atggggcccg acggctcctc gaatttcgac gtgtacctgc gcacggcggt
    13081 gagcgtcgag ccgcagggtt gggcggagtt cggttatgtg aagatgcccg attaccgctg
    13141 gggcatcttc ctcacccccg tggccggcgg cgcgagcatc ggcttcggcg agcacaaggg
    13201 cgcgccggtg tggcatgacg tccccggcga gcatcgcgcg aacctgcggc gcattgtcgt
    13261 cacgcagggc gacaccgagc ctgcgtccgt cgagcagcag cgccacctcg gcctcacctg
    13321 tccgtcgctc tacgatctgc gcaacctgtt ccaggtcaac gtcgaggaag cccggcacct
    13381 gtgggcgatg gtgtacctgc tgcaccgcta tttcgggcgc gacggacgcg aggaggccga
    13441 ggcgctgctg acgcgccgct cgggcgacgc cgacaatccg cgcatcctcg gcgcgttcaa
    13501 cgagcgcaca cccgactggc tgtcgctgtt catgttcacc tacttcaccg accgcgatgg
    13561 caagttccag ctcgcggccc ttgccgagtc gggtttcgag ccgcttgcgc ggaccacgcg
    13621 gttcatgctg accgaggaag cgcatcacat gttcgtcggc gagtcgggtg ttgcgcgcgt
    13681 catccagcgc acgtgcgacg tcatgaagca aatgcagatc ggagaccctg ccgacgtgcg
    13741 ggcgcagggc gtcgtcgatc tcgacaccat ccagcgctac ctcaatttcc attacagcgt
    13801 cacgatcgac cttttcggcg ccgaccagtc gtcgaacgcg gccacgttct acacggcggg
    13861 gctcaaggga cgctacgagg aagcgaagcg cgacgacgac cacgtgctga aggggcgcag
    13921 ctacccggtg ctcgatgcac gcgacgggcg cctcgtcgac cgcgacgtgc cgatgctcaa
    13981 tgcgctcaac gaagtgctgc gcgacgattt catccgcgat tcggtggccg gcatcggacg
    14041 ctggaaccgc gtgatcgaga aggccggtat cgcgtttcgc ctgcaggttc cgcacaaggc
    14101 gtttcatcgc aagatcggat cgctggcgca gcttcgcgta gcccccgacg gcagcgtggt
    14161 gtcggacacc gagtgggacg cgaacgtgca tcgatggctt ccgaccgccg acgaccgcgc
    14221 atttgtcgcg gcgctgatgg ggcgcgtcgt cgagcccggc cggttcgcca actggatcgc
    14281 cccgcccgcc gccggcgtca accgtcaacc ggccagtttc gagtacgtgc ggttcaattg
    14341 aacgacgcca cggcgaccga ggagcagcgg atgcgcgccg catcgagccc cgattacgct
    14401 ttgcagcacc tgatcgatcc ggaggtgtgc atccgctgca acacgtgcga ggcgacctgt
    14461 ccgatcggcg ccgttacgca cgatgcccgc aactacgtcg tcgatttcgc caagtgcaac
    14521 gcgtgcaatg cgtgcctgcc gccgtgtccg accggcgcca tcgatcactg gcaggcggtg
    14581 cgcaagagcg caccgtacac gctcgccgat cagtacgact gggacagcct gccggcgccg
    14641 gtggcagtcg aggcggggca tgccggggaa ataccggcgg aagtgcggca actggccgag
    14701 gtcgcgagcg cggggcaggc cggtgcgacg tctgcgccgt ggtcggcggc acagccgtgc
    14761 gtcaacctgt acctgccggc acagccggcg atcgcacgcg ttaccggcaa ctatcggctg
    14821 actgccgtgg atgcctcggc cgacattcgc catatcgtgc tcgacttcgg cagcaccgcc
    14881 tttccggtgc tcgagggcca gacgctcggc atcctgacgc cgggcgagga tgcgcacgga
    14941 aagccgcact acgtgcgcct gtattcggtc gcgagtccgc gcgacggcga gcggcccggc
    15001 cacaacaaca ttgcgttgac cgtgaagcgc gtgaccgtcg atcacgacgg acgcgcggtg
    15061 gccggggtcg cgtcgaatta cctgtgcgat ctcgcgcttg gcgacgaagt gaaggtggtc
    15121 ggtccgtacg gaacgagcta tctgatgccc gatcacccgg gttcttcact gatgatgatc
    15181 tgcaccggca ccgggtcggc gccgatgcgc gcgatgaccg agcggcgacg ccgacgccgg
    15241 atgttgaagg aaggcggcga gctcctgctg ttcttcgggg cgcgcgtcga gcatgagctg
    15301 ccgtacttcg gcccgctcac gaaactcccc tgcgaattca tcgatgtgaa cttcgcgttt
    15361 tcgcgcgttt ccgggcagcc gaaggtgtac gtgcaggacc gcattcgcga gcgtgcggcg
    15421 agtgtggcgc gcatgctgca ggacgacgac tgctacatct acgtgtgcgg gctgcgtggg
    15481 atggagaagg gcgtcatcga cgcgttccgc gacgtctgcg acacgcacgg tgtcgagtgg
    15541 aatgcattgc tgccggcgct gcagtcaacg ggccggttgc atatcgaaac gtactgagtg
    15601 cgcgccgcgc acgacggccg gctacggggt cagtgcaacg gcgaggggta tccagaatcc
    15661 gagcatcgaa acgaggattg cgcacaggat gccgcgcatc gcatccagcg catcgtccgc
    15721 actggtcgtc cgtcgccact cgagctgcga catccgaacc gggtgtacga gcgtttgtcc
    15781 catgatcacc tcgcaatgag gtcagatgac ccgagcttgc cgccagcggc aaatgcgcgc
    15841 aatgcgcaag ccgccggttc cgctgtagga cgatcgcggc ggggccttct tgtactgacc
    15901 ggcaaaccgg ggtaggcggc ctcctacccg acgtcccgcg cgttgccgat ggccccgggc
    15961 gccactgccg gctaggatca ttgaatggcg ccgcacggcg gcgcgtcgaa ccgaaggagc
    16021 gaacatgcgc aaggcgctcg ctgtgcttgc aatacttctg tgctcggcca cgccgtcgct
    16081 tgcgcaagtg agcctcggct tcaacagccc cggtgtgagc atcggcgtca atctgccggt
    16141 gtatcccgac cttgtgcgca tcccgggcta tcccgtctat tacgcgccgc aggtgaatgc
    16201 gaattacttc ttctacgacg gcctctactg ggtattcgac ggcgtcaact ggtacgcgag
    16261 cacctggtac aacgggccgt ggcagctggt gacgagcgac gccgtgccgc tgttcctgtg
    16321 gcgcgtgccg gtgcgctact accgcgagcg gcccgcctac tttcgtggct gggcgctgaa
    16381 cgcgccgccc cgctggggcg cgcattgggg caacacgtgg gcgcaacgcc atcgcggctg
    16441 ggatcgctgg aaccgcgcat cggtgccggc cgcggcgcct ttgccaacct atcaacggcg
    16501 ctatgcgggg aaccggtatc cacgcggcga tgagcagcga gcgctcgaga cccgcaacta
    16561 tcgttatcaa ccgcgcgagc gcgtcgcgca gcagcacttc cagcagcgtt cgacgcagag
    16621 cacgccggct gcgcggccgc agggaagaac gggcttcgcg gagaacggac gcgagcgcgg
    16681 acgcgagcgc aacgggatcg atcgcacgca gaatgcgaat cggaacgtgc aacgcgtcgc
    16741 accgcccgcg acggcgcaac gcggcggccc gccggttacg gcgcaacgcg ccgcgccgcg
    16801 cgagcgaggc gcgccgccca cggcaaggcg cgcgggtcct ccggacgttg agaatggcgc
    16861 gcctcgcgcc gcgccacctc gcggcgaggc acgcgctgcg caggggcgag gcccgcaggc
    16921 cagggagccc gcgcaggcgg cgaaccggcg cggcaacagc ggcgctgcac atgcacgggg
    16981 tgacgagcgc cgggacgagc acaacggata gccgcgggtt cggtccgctc gtccggtccg
    17041 cctccagggc cacgcgtttt cgcgtggcct tttcgcagtc cgccccggcg gcagggggtc
    17101 ggtgcgcccg aattcaaccg caaggcccgg agtgcgcggg ggcgatggga atcttaaggt
    17161 gggagcctcg ataatgcgaa agagcgaggg atccacgatg accacccagg caatcaacga
    17221 cacggccgcg gcacgcacgc aaagcgatcc gcgctgggcc gccgtcgtcg cacgcgacgc
    17281 gcgtgcagac ggcacgttct tctattcggt gcgaacgacc ggcgtctact gccgcccttc
    17341 gtgcgccgcg cgtgttgcga ggcccgagaa cgttgcgttc cacgccagta cgcacgacgc
    17401 ggagcgcgcc ggatttcgtc cgtgcaaacg ctgtcgtccc gatcggctgt cgccgtcgca
    17461 ggcgcaggcg gcgctcgttt cgacgctttg ccggttcatc gaagcggccg atcacgtgcc
    17521 gacgctcgcg gagcttgcga cgcgcgccgg gttgagcgcg catcatctgc atcgcacgtt
    17581 ccgtgcgatc accggcgtga cgccgcgagc ctatgcggct gcgcatcgcg cgcgccgggt
    17641 ccgggatgcg ctggcggcga gcacgacggt gaccgatgcg atctacgacg cggggtatgg
    17701 ctcgaatgga cgtttctacg acgaagcgaa cggggtgctc ggcatgacgc cgacgcgctt
    17761 tcgtgcgggt ggcgccggga cacgcattca ctttgcgatc ggcgaatgct cgctgggatc
    17821 gatcctggtc gcgcaaagcg agcgcggcgt gtgcgcgatc ttcctcggcg acgatcccga
    17881 ccggctggcg cgcgatcttc aggatcgctt cccgaatgcg cagctcgtcg gcggcgacgc
    17941 gacgttcgaa ggcgtggtcg ccaaggtcgt cgggttcgtc gagtcgcccg atgcccggct
    18001 cgacctgccg ctcgatgtgc gcggcaccgc gttccagcgg cgcgtatggc aggcgatgcg
    18061 cgagatcgcg ccgggcgaga cggcgagcta cagcgagctt gcacggcgca tcggtgcacc
    18121 gaagtcgacg cgcgcggtgg cggccgcgtg cgccgccaac gtgctcgcgg tcgcaatccc
    18181 ctgccaccgc gtggtgcgca gcgacggtgc gctgtccggc tatcgctggg gggtcgagcg
    18241 caagcgcgcg ctgatcgagc gcgaggcgcg caaatgatgg ccgcgtcgcc atgaatgcgc
    18301 gcgcgaacga tgaagcggtt cgcgccgcgg cgggcggcat cgagccgcgg atcgacgcgg
    18361 tggattgggc gcgcgtcgcg gtcgagctcg acgcgcaggg cagctcggtg atcgagcgcc
    18421 tgctcacacc cggcgagtgc gcttcgctcg tgtcgctcta tggaagcgac gcggcgtttc
    18481 gcggccgagt ggtcatggct cgccatggtt tcggtcgcgg cgagtacaag tacttcgcct
    18541 atccgctgcc ggctgttgtg gcgaatctgc gcagcgcgct ctactcgcgg ctcgcgccga
    18601 tcgccaaccg gtggtacgag gcgatgcgca tcgacgtgcg ctttcccgca cggcacgatg
    18661 agttcctcga gcgatgccac gcggccggcc agcgccggcc gacgccactg ctgctgcagt
    18721 acggtgcaga cgattacaac tgcctgcatc aggacctcta cggcgcgcag gtctttcctc
    18781 tgcagatcac gatcctgctg tcggaaccgg gtcgcgattt cgacggcggc gagttcgtga
    18841 tgaccgagca gcgcccgcgc atgcagtcgc gtccgcatgt ggtgccgctg cggcaggcag
    18901 acgcggtcgt gtttgccgtc catcagcgtc cggtgcgcgg tgcccgtggc ttttatcgcg
    18961 tcaatctgcg ccatggcgtc agccgcgtgc gcaccgggca tcgccatacg gccggtatca
    19021 tcttccacga cgcggcgtga ggacacgcga tctgttcgac actgcggcgc gcggcccgta
    19081 cgtggaaacg ctcgcgccgg gtgcggcgat cctgcgcggc ttcgcgcggc cgttcgagga
    19141 cgcgctgatc gacgcgctga acgacgtcgt cgcgtcggcg ccgcttcgcc acatggtgac
    19201 gcccggcggt ttccgcatgt cggtcgcgat gacgaactgc ggctcactcg gctgggtgtc
    19261 gagtgcgacg ggctaccggt acgacgcggt cgatcccgac aacggcaacc gctggccgcg
    19321 gatgccgcgc gcgtttctga ggctcgcaaa ggacgccgct gcacgcgccg gattcgccgg
    19381 cttcgtgccc gacgcctgcc tcgtcaaccg ctacgcgccc ggtgcgcggc taacgctgca
    19441 tcgggaccgt aacgagcggg atttcgaagc gcccatcgtg tcggtatcgc tcggcctgcc
    19501 ggccacgttt cttttcggcg gcctcgcgcg ctcggacagg ccgcttcgag tgcaactgca
    19561 tcacggcgat accgtcgtct ggggcggacc tgcccggctt cgctaccatg gtgtggccgc
    19621 gctcgaggcc ggcgagcacg accggatggg tgcgcagcga atcaacctga cgttgcgcaa
    19681 ggccggatag ccgcgcagcg gatcagagcg gcttgatttc cactttccgg aacttgacga
    19741 cgccggccgc ccactgcagt ccgatgttgc cgcgaagtgc gttcgccttg tcggtttcac
    19801 ccgtctgcac gccgttcatc acgacgacga tccgcggacc ccgcgcggtg atctcgtagg
    19861 tattccattt gccgcccgcc ttcggcatgt tcgtgatcgc cgaaagatgc gtgatcgcgc
    19921 cggtcccgta ggtcgggtcg ggccgctggt cgaagatatt cgcttccgtg cagttggtgt
    19981 ccttgatgtc gcgaagatcg ggacagcgca tgtagatgcc ggaattggca tcgtcgctga
    20041 cccagaattc cgcgcgcagc atgaaatcgc cgtacgcgtt cttcgtcatc aggaagctgc
    20101 tcttcccctt ttcaccggtg tccgcctgcg cgacgccatc gacgatgcgc cagttcgcgt
    20161 cgccgatgcg atcccagttt tccgtgaagc ctttcgggcc gtcgagcagc gtgacccatc
    20221 cggttgccgg cgactgcggc gacatatcgg cacagccggc gctgacgccg acgaggaggg
    20281 cgatgacgat gaggcaaacg cgtttcatgg gaagcctccc gggtcgagat acgcggcggc
    20341 aggatgcggt acggcgaccg tcgcgtcaag ggccgggaga tgggccggga agtggcctgg
    20401 tcggccggta tccgccggca tgttcgtcgg caggaccgcg cggtttgcta aggtgtcggc
    20461 cggattcgac tgtcggcctt tggacaacgt cactcacacc ctgatcggcg cgctcctcgg
    20521 cgaatccgcc gctcgcctcg cgccccccgc cggcaacggg cggcaggcga agctgcgccg
    20581 cgggctgttc ctggcggtcg cgatcgtcgg cagcaatttc cccgatgccg acctgctgtc
    20641 cacgctcggt gcggacggca agctcgcgta tctgctcgag catcgcgggc acacgcacac
    20701 ggtcgctggc gcgcttctga tctcgcttgc aatgctcggc gggtgcgcac tgtggcagcg
    20761 gtggcgtggc ctcgtgctgc ccgcgcgcga ccgccggtgg ctcgtggccc ttgcgctgct
    20821 cgcgccgctc tttcacatcg cgatggatgc gttgaacacc tacggcgtgc atccgtggtg
    20881 gccgttcgac aatcgctggt actacggtga cgccgtattc atcgtggaac cgctgttctg
    20941 ggccgccgca gcgccgctcg cctttctgct caggagctgc acggcacgcg cattcgtcgt
    21001 gctcgc
//
LOCUS       DASSVX010000090         5699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_919575, whole genome shotgun sequence.
ACCESSION   DASSVX010000090 DASSVX010000000
VERSION     DASSVX010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919575"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..877)
                     /locus_tag="VFO53_04145"
     CDS             complement(5..877)
                     /locus_tag="VFO53_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8875500.1"
                     /translation="MSPSITILAQHLLNGVLIGCAYALIAMGLTMIFGVMNIANFAHG
                     EFYMLGGFVAFYLVSLMGLNYFAALPLAVVAVVALAIVLQHSIFRRLREAPMMTTTLV
                     TIGLSIFLENFALLLWGPQPQSIPTPFPTKPLSFLGVYTTPLYVFCMAVTAALIVVGH
                     VLIRRTRMGKAMRATFQNKEAAALVGIRIDRIYAYAFAFGAALAAAAGAVLGGFIPVE
                     PATGGIVTLKAFIVVILGGMGSFVGAIVGGLVLGLAEALGAGYMPSGWKDAIGFFAVI
                     LVLLFRPEGLFKRA"
     gene            complement(906..2039)
                     /locus_tag="VFO53_04150"
     CDS             complement(906..2039)
                     /locus_tag="VFO53_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8875501.1"
                     /translation="MHTLRVALAVAVSLALAPLAVAQETVRIGVVQPTTGPVAYDGNI
                     YVNTVKMLVDDMNAKGGVLGKKIELVVEDGACNPAQSVNAAEKLVVRDKVVALLGAFC
                     STSTAAVMEVAKKHKVPLITGISTAAQLTEQGNPYFFRAVATTPMLGNAFGGVLPKAV
                     KAKRFAFLVLNDDWGRSMAASYPKTIENAGGQVVATEFFQQSDLQFLSQITKVKAANP
                     DAIVLAANTQQAVALSKQIRELGVSVPLIGEGAWTSDSYIKLAGPASEDVYGLVEYVY
                     TIKTPINEAFVKAFQAKLKENPSKFAGAAHNAIHIMVDAIGRAGAADPEKIRDALEKT
                     DYNGIVGNIRFDAKHQAYGQTVYLAHVKNGVPQVVETAQIAKP"
     gene            complement(2178..2921)
                     /locus_tag="VFO53_04155"
     CDS             complement(2178..2921)
                     /locus_tag="VFO53_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8875502.1"
                     /translation="MHAKNNRPHYAVGPSRKNAARQNGASGNTLTDRIRQAVEHDIVS
                     GRLAPGTKLDEDRLASRHGASRTPVREALQHLASRGLIELRPHAGAFVPERSVVELAE
                     MFEAMSFLEAACATLAARRHSAADRSLLAAAHDACASAAKRDDPVAFYAANAHFHGRI
                     YAAAHNEYLASQTIQLGKRLEAYRREATFHPGLMSQTMAEHERILKAVLAMDEAAAGA
                     QMRGHLDTLRDDVVSMATAMRGIGRENVR"
     gene            2995..3798
                     /locus_tag="VFO53_04160"
     CDS             2995..3798
                     /locus_tag="VFO53_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8875503.1"
                     /translation="MDLRLAGRWVLITGASKGIGLAAARAFAVEGCNLHLAARSGEAL
                     DAARIEIKNRCGVSVEVHVTDLSCTDNVVELGKRCAGVDILVNNAGEIPSGPIEALKE
                     EDWRRGYDLKLFGYITLTREIYAAMKARGSGVIINDIGNSGENWDANYIAGSTANAAL
                     MAFTRALGGQSLDFGVRVVGVNPGPVETERMIKINRRRALDWYGDESRWQELREKFPG
                     GRPASAEEVADLMVYLASPRAGYITGTIVTIDGGIAARGSIIKERSARG"
     gene            3803..4936
                     /locus_tag="VFO53_04165"
     CDS             3803..4936
                     /locus_tag="VFO53_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET8875504.1"
                     /translation="MKQFEIRNRHFDRLFNTPGLMWLGQNTNHFSPHPAVVDAMHDAI
                     GREEYHAYAPPAGFEELRALILDDLGLPEQSVLVTDGAVEALYNVCHTVCTPGSHFVT
                     TDPGWKWPIHFAQAAGATTTEIPIYKPENGYKLTVAQLEAAVTDRTRLIYLVDPNNPL
                     GTCHTRDEIEAFARIARDAGAYFMHDCTYAHFADDHTLAARFHPEATFTIYSFSKWLG
                     VAGLRIGAVVADPGLIERLAVAPPNNLGSNVISQRAAIAGLRTKSEWFPDINRRQRRN
                     QAQIVKAVDRVPGMKVAVYPSQANFLVVECIEAGIKPEALCDAYRSEDIMIRQGTYHT
                     PTFGHRFVKVSTTVPEAWADAFCERLPAMVEKARGITQMAPLF"
     gene            4950..>5699
                     /locus_tag="VFO53_04170"
     CDS             4950..>5699
                     /locus_tag="VFO53_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8875505.1"
                     /translation="MSYARSEDGTRLYYEEAGSGSPIVFVHEFGGTYRSWEPQMRALS
                     RRHRCITFAARGYPPSDVPENADRYSQALAVADIAAVMDAAGVDRAHIVGLSMGGFAA
                     LHFGLDHPGRALSLVVAGAGYGAEKQFETYFRDVSLEVARQFEAQGSEQFAKTYALGA
                     SRVQFQNKDPRGWAEFAALLAQHSAPGAANTMRGVQALRPSIYDLEDRLRTMAVPTLV
                     IVGDEDDHCIQPGVFMKRTIPACGLLILPKTG"
BASE COUNT          994 a         1832 c         1895 g          978 t
ORIGIN      
        1 cggttcaagc acgcttgaag aggccttcgg gccggaacag caggacgagg atcaccgcga
       61 aaaatccgat cgcgtccttc cacccgctgg gcatgtaacc cgcgccgagc gcctcggcga
      121 ggccgagcac gaggccgccg acgatggcgc cgacgaagct gcccatgccg ccaaggatca
      181 cgacgatgaa cgccttgagt gtcacgatgc cgccggtggc gggctcgacc ggaatgaatc
      241 cgccgagaac cgcaccggcg gccgcggcga gcgccgcacc gaacgcaaag gcgtaggcgt
      301 agatgcggtc gatgcggatg cccacgagcg ctgcggcctc cttgttctgg aacgtggcgc
      361 gcatcgcctt gcccatgcgc gtgcgccgga tcagcacgtg cccgacgacg atcagcgccg
      421 cggtgacggc catgcagaaa acgtacagcg gcgtcgtata aacgccgagg aagctcagcg
      481 gtttcgtcgg gaacggcgtc ggtatggact ggggctgcgg gccccacagc agcagcgcga
      541 aattctcgag gaagatggag agcccgatcg tgacgagcgt tgtcgtcatc atcggcgctt
      601 cccgcagccg gcggaaaatc gaatgctgga gcacgatcgc cagcgcgaca accgcgacaa
      661 cggcgagcgg aagggccgcg aagtaattga gccccatcag gctcacgagg tagaacgcga
      721 cgaatccccc gagcatgtag aactcgccat gcgcgaagtt cgcgatgttc atcacgccga
      781 agatcatcgt gagtcccatc gcgatcaacg cgtaggcgca accgatcaga accccgttca
      841 gcagatgctg ggcgaggatg gtgatcgacg gcgacatgcg gcgcgcggtc cgggcggccg
      901 cttcgctacg gcttggcgat ctgcgccgtc tccaccactt gcggcacgcc gttcttcacg
      961 tgcgcgaggt agaccgtctg gccatacgcc tggtgcttcg catcgaacct gatgttgccg
     1021 acgataccgt tgtagtcggt cttttcgagg gcgtcgcgga tcttttcggg atcggctgcg
     1081 ccggcgcggc cgatcgcgtc gaccatgatg tggatggcgt tgtgggccgc tcccgcgaat
     1141 ttcgacgggt tctccttgag ctttgcctgg aacgccttga cgaacgcttc gttgatcggc
     1201 gtcttgatcg tatagacgta ctcgacgagc ccgtacacgt cttccgacgc gggaccggca
     1261 agcttgatgt agctgtcgct cgtccacgcg ccttcgccga tcaatggaac actgacaccg
     1321 agctcgcgaa tctgcttgct gagcgcaact gcctgctgcg tattggcggc cagcacgatc
     1381 gcgtcgggat tggcagcctt gaccttcgtg atctgcgaca ggaactgcag gtcgctctgc
     1441 tggaagaatt ccgtcgcgac gacctggcca ccggcatttt cgatcgtctt cggataggag
     1501 gccgccatcg accggcccca gtcgtcgttc aggacgagga acgcgaaacg cttcgccttg
     1561 accgccttcg gcaggacgcc gccgaacgca ttgccgagca tgggcgtcgt ggccaccgcg
     1621 cggaaaaagt agggattgcc ctgttccgtc aattgcgcgg ccgtcgagat gccggtgatg
     1681 agcgggacct tgtgcttctt cgccacttcc atgacggcgg cggtcgacgt gctgcagaac
     1741 gcgccgagca aggcgacgac cttgtcgcgc acgacgagct tctccgcagc gttcaccgac
     1801 tgcgccggat tgcacgcgcc gtcctcgacg acgagctcga tcttcttgcc gagcacgccg
     1861 cccttcgcgt tcatgtcgtc gacgagcatc ttgacggtgt tcacgtagat gtttccgtcg
     1921 tacgcgaccg ggcccgtcgt cggctgcacg acgccgatgc ggacggtttc ctgcgcgacg
     1981 gccagcggcg cgagcgcgag cgagacggca acggccagtg cgaccctgag cgtgtgcatg
     2041 cggatttccc ctcgtggttg tcgggtgcgg acgataatat acgagaataa tcggatcgta
     2101 tatcaggcgc agccgtctgc cctgtctcgt cacgcgatcg tcggcggtgc gcgcgacgac
     2161 gtgaagcgca ataccgctta ccgtacgttt tcgcggccga tgccacgcat cgccgttgcc
     2221 atcgacacga cgtcgtcacg cagcgtatcg aggtggcctc gcatctgcgc cccggccgcc
     2281 gcctcgtcca tcgcgagcac ggctttcaga atgcgctcgt gttcggccat cgtctgcgac
     2341 atgaggccgg gatggaacgt ggcttcgcga cgataggctt cgagccgctt gccaagctgg
     2401 atcgtctgcg acgcgagata ctcgttgtgc gcggcggcgt agatgcggcc atggaaatgg
     2461 gcgttggcgg cataaaacgc caccggatcg tcgcgcttcg cggcgctggc gcatgcgtcg
     2521 tgagccgctg caagcagact gcgatcggcg gcgctgtggc gccgcgcagc caacgtggcg
     2581 cacgccgctt cgagaaacga catcgcctcg aacatctcgg cgagctcgac cacgctgcgc
     2641 tccggcacga acgcgccggc gtgcggccgc agctcgatca ggccgcgcga cgcgagatgc
     2701 tgcagcgcct cgcgcacggg cgttcgcgat gcgccgtgcc gcgacgcgag ccggtcctcg
     2761 tcgagcttcg ttcccggcgc aaggcgaccc gagacgatgt cgtgctccac cgcctggcga
     2821 atgcggtcgg tcagcgtgtt gcccgacgcg ccgttctgtc gcgcggcgtt cttccggctc
     2881 gggccgactg cgtagtgcgg ccggttgttt tttgcgtgca tccgttcggt ggtgagcggg
     2941 gcttgaccga cgattgtatg cgacactatc cttttcgtat acaagaggtg ccggatggat
     3001 ctgcgactcg cgggccgatg ggtgttgatc acgggggcgt cgaagggcat cggccttgcg
     3061 gcagcacgcg ccttcgcggt ggagggctgc aacctccacc ttgcggcgcg atcgggcgaa
     3121 gcgctcgatg cggcgcgcat cgaaatcaag aaccgatgcg gcgtcagcgt cgaggtccac
     3181 gtgacggacc tgtcgtgcac cgacaatgtc gtcgagctcg gcaagcgctg cgccggcgtg
     3241 gatatcctgg tcaacaacgc gggcgagatt ccgtcgggcc cgatcgaggc gctgaaggag
     3301 gaagactggc gccgcggcta cgacctcaag cttttcggct acatcacgct gacgcgcgaa
     3361 atctacgcag cgatgaaggc gcgcggatcg ggcgtgatca tcaacgacat cggcaactcc
     3421 ggcgagaact gggatgcgaa ttacatcgcg ggcagcacgg cgaatgcggc gctgatggcg
     3481 ttcacccgcg cgcttggcgg ccagagcctc gacttcggcg tgcgcgtcgt cggcgtcaac
     3541 ccggggcccg tcgagaccga gcggatgatc aagatcaaca gacgccgcgc gctcgactgg
     3601 tatggtgacg agagccgctg gcaggagctg cgcgaaaagt ttcccggtgg acgcccggca
     3661 agcgcagaag aggtggccga cctcatggtc tacctcgcgt cgccccgcgc cggttacatc
     3721 accggcacga tcgtcaccat cgacggcggc atcgccgcgc ggggctcgat catcaaggaa
     3781 cgcagcgcgc gaggatagtc caatgaagca gttcgaaatc cgcaaccggc acttcgaccg
     3841 cctcttcaat acgccagggc tcatgtggct cgggcagaac accaatcact tttccccgca
     3901 ccccgcggtc gtggatgcga tgcacgacgc gatcggccgc gaggagtacc acgcgtacgc
     3961 gccgcccgcc ggattcgagg agctgcgggc gctgatcctc gacgatctcg gcttgcccga
     4021 acagtcggtg ctcgtcaccg acggcgcggt cgaagcgcta tacaacgtct gtcacaccgt
     4081 ctgcacgcca ggcagtcatt tcgtgacgac cgatcccggc tggaagtggc cgatccattt
     4141 cgcgcaggcc gcaggtgcga cgaccaccga aattcccatc tacaagcccg agaacggcta
     4201 caagctgacg gtcgcgcagc tcgaggcggc ggtgaccgac aggacgcggc tcatctatct
     4261 cgtcgatccg aacaatccgc tcggcacgtg ccatacccgc gacgaaatcg aagcgttcgc
     4321 gcgcatcgcg cgcgacgccg gcgcgtactt catgcacgat tgcacgtatg cgcatttcgc
     4381 cgacgaccac acgctcgcgg cgcgcttcca tcccgaggcg acgttcacga tctacagttt
     4441 ctcgaagtgg cttggcgttg cggggctgcg catcggcgca gtcgtcgcgg atcccggcct
     4501 gatcgagcgc cttgccgtgg ccccgccgaa caatctcggc tccaacgtga tctcgcagcg
     4561 cgcggcgatc gcgggcctgc gaacgaagag cgagtggttt cccgacatca atcggcggca
     4621 gcggcgaaac caggcgcaaa tcgtgaaggc ggtcgacagg gtgcccggga tgaaggtcgc
     4681 ggtgtatcca tcgcaggcca acttcctcgt cgtggagtgc atcgaggcgg gcatcaagcc
     4741 cgaggcgctt tgcgacgcct atcgatcgga agacatcatg atccgccagg gcacgtatca
     4801 cacgccgacc ttcgggcatc gcttcgtcaa ggtgagcacc acggtgcccg aggcgtgggc
     4861 cgatgcgttc tgcgaacgcc tgccggcgat ggtcgagaag gcgcggggca tcacgcagat
     4921 ggcgccgttg ttctagcgag gggaacgaga tgagctacgc gcgcagcgaa gacggtacgc
     4981 gtctctacta cgaagaggcg ggttcgggct cgccgatcgt gttcgtgcac gaattcggcg
     5041 gcacctaccg cagctgggag ccgcagatgc gcgcgctttc gcgccggcat cgctgcatca
     5101 cgtttgcggc gcgcggctac ccgccgtccg acgtgccgga gaacgccgat cgctattcgc
     5161 aggcgcttgc cgtcgcggat atcgcggcgg tcatggatgc cgccggcgtc gaccgtgccc
     5221 acatcgtcgg actgtcgatg ggcggcttcg ccgcattgca cttcggtctc gatcatcccg
     5281 gccgggccct gtcgctggtc gttgcgggcg cgggatacgg cgccgagaag cagttcgaga
     5341 cgtacttccg tgacgtgtcg ctcgaagtcg cgcggcaatt cgaagcgcag gggagcgagc
     5401 agttcgcgaa gacgtatgca ctgggtgcgt cgcgcgtcca gttccagaac aaggatccgc
     5461 gcggttgggc cgaattcgcc gctctcctcg cgcagcattc ggcgcccggt gcggcgaaca
     5521 cgatgcgtgg cgtgcaggcg ctccggccgt cgatctacga tctcgaggac cggttgcgca
     5581 cgatggcggt cccgacgctc gtgatcgtcg gcgacgagga cgaccattgc atccagccgg
     5641 gcgtgttcat gaaacggacc attcccgcgt gcgggctgct gatcctcccg aagaccgga
//
LOCUS       DASSVX010000091         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1102864, whole genome shotgun sequence.
ACCESSION   DASSVX010000091 DASSVX010000000
VERSION     DASSVX010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102864"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VFO53_04175"
     CDS             complement(<1..1050)
                     /locus_tag="VFO53_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8875506.1"
                     /translation="MAQYVYTMNRVGKLVPPKRVILKDISLSFFPGAKIGVLGLNGAG
                     KSSLLRIMAGEDKEYEGDAVPMPDLRIGFLPQEPRLDPDATVRSVVEQGMGEVLQAKA
                     RLDEVYAAYAEPDADFDALAAEQARLEAIIAASGADAGVQMEIAADALRLPPWDATIG
                     TLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVEWLEQYLAKFPGTVIAVTHDR
                     YFLDNAAEWILELDRGYGIPYKGNYSLWLEQKEQRLETEAKQEAARIKAMKQELEWVR
                     QNAKGRQAKSKARIARFNDLASYEHQQRNETNEIFIPVAERLGDAVIEFENVRKAYGD
                     KLLIDDLSFRVPAGAI"
     gene            complement(1156..1884)
                     /locus_tag="VFO53_04180"
     CDS             complement(1156..1884)
                     /locus_tag="VFO53_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET8875507.1"
                     /translation="MNASAYRIDRKSAACHVGALALALLFAFVPGRAHAELYKWTDAR
                     GEVHYSDKLPVEAVNGATTQLNRQGITVRKTEAARPIAQQPVAHTENEEQKLREEQRA
                     KVLAVRRDRALIESYASEAEIDLAKSRAVATIDGQVRSANAYVAQMTKRRQELEDKKP
                     TYAPRPVPGSLVREIETIDAELGRQNEFIAAKEKEAASVAARYEADKQRFRELRAGAS
                     GSVATTDSRSAATSAVGITLTSAR"
     gene            complement(1919..2563)
                     /gene="pyrE"
                     /locus_tag="VFO53_04185"
     CDS             complement(1919..2563)
                     /gene="pyrE"
                     /locus_tag="VFO53_04185"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET8875508.1"
                     /translation="MSDFRREFLQFALAHDVLRFGEFMTKAGRKSPYFFNAGLFCDGA
                     SLGRLGEFYAQALLESGVDCDQLFGPAYKGIALASATAIALAARGHNLPYAFNRKEAK
                     DHGEGGSVVGAKLRGRVMIVDDVITAGTSVRESVALIRANGAEPAGVLIALDRMERGQ
                     GDVSAVQEVRDVHAIPVVAIATLDDVMAFIAKRPELAAHATAVTEYRARYGAAR"
BASE COUNT          407 a          913 c          831 g          494 t
ORIGIN      
        1 gatcgcgccc gccggaacac ggaacgacag atcgtcgatc aggagcttgt cgccgtacgc
       61 cttgcgcacg ttctcgaact cgatgacggc gtcgcccaat cgctcggcaa ccgggatgaa
      121 gatctcgttc gtctcgttgc gctgctggtg ctcgtacgac gcgaggtcgt tgaaccgggc
      181 gatgcgcgcc ttgctcttgg cctgccggcc cttggcgttc tggcgcaccc actcgagctc
      241 ctgcttcatc gccttgatgc gggcggcctc ctgcttggct tccgtctcga ggcgctgctc
      301 cttctgctcg agccacaggc tgtaattgcc cttgtacgga atgccgtagc cgcggtcgag
      361 ctcgaggatc cactcggccg cgttgtcgag gaagtagcgg tcgtgcgtca ccgcgatcac
      421 ggtacccgga aacttcgcga ggtactgctc gagccactcg acgctttccg catcgagatg
      481 gttcgtcggc tcgtcgagca gcagcatgtc gggcttcgac agcagcagcc ggcacagcgc
      541 gacgcgccgc ttctcgccgc ccgacagcgt gccgatcgtc gcgtcccacg gcggcaggcg
      601 cagcgcgtcg gcggcgatct ccatctgcac gccggcgtcg gcgcccgacg cggcgatgat
      661 cgcctcgagc cgcgcctgct cggcggcgag cgcgtcgaaa tcggcgtcgg gctccgcgta
      721 cgccgcgtac acctcgtcga ggcgcgcctt cgcctgcagc acttcgccca tcccttgctc
      781 gacgacgctg cgcaccgtcg cgtcgggatc gagcctcggc tcctgcggca ggaagccgat
      841 ccggagatcg ggcatcggca ccgcgtcgcc ctcgtactcc ttgtcctcgc cggccatgat
      901 ccgcagcagg ctcgacttgc cggcgccgtt caggccgagc acgccgatct tggcgccggg
      961 aaagaacgac agcgaaatgt ccttcaggat gacgcgcttg ggcggaacga gcttgcccac
     1021 acgattcatc gtgtaaacgt actgagccat cggatttgcg aagcgggttt gccgaaccgc
     1081 gattatcgca gcggcaccga tgcagggtcg gagacgactt tccgcgaaga aaaccaaagc
     1141 agggtcagag accctttatc gggcgctggt cagcgtgatg ccgaccgcgg atgtcgcggc
     1201 gctgcggctg tcggtggtcg cgacggagcc gctggcgccg gcgcgcagtt cccggaagcg
     1261 ttgcttgtca gcctcgtaac gcgctgcgac gctcgccgct tccttttcct tggcggcgat
     1321 gaactcgttc tggcgcccca actccgcgtc gatcgtctcg atctcgcgga cgagggagcc
     1381 ggggaccggg cgcggcgcat acgtcggctt cttgtcctcg agctcctgcc ggcgcttcgt
     1441 catctgcgcg acgtatgcat tggccgatcg cacctggccg tcgatcgtcg cgaccgcccg
     1501 cgacttggcg agatcgatct ccgcctcgct cgcgtacgac tcgatcagcg cgcgatcgcg
     1561 ccgcacggca aggaccttcg cgcgctgctc ttcgcggagc ttctgctctt cgttctcggt
     1621 gtgcgcgacc ggctgctgcg cgatcggccg cgccgcttcg gtcttgcgca cggtgatgcc
     1681 ctggcgattc aactgcgtgg tcgcgccgtt gaccgcctcg accggcagct tgtcgctgta
     1741 atggacttcg ccgcgggcgt ctgtccactt gtagagctcc gcgtgcgcgc ggcccggcac
     1801 gaaggcgaac agcagagcca gtgcgagcgc gccgacgtgg cacgccgccg acttccgatc
     1861 gatccgatac gccgatgcgt tcatgacgat ccttcgcttg gcgccgtcca ccggcgcctt
     1921 atcgcgccgc cccgtagcgg gcacgatact cggtcaccgc ggtcgcgtgt gcggcgagct
     1981 cgggacgctt cgcgatgaac gccatcacgt cgtcgagcgt cgcgatcgcg accaccggaa
     2041 tggcgtgcac gtcacgcact tcctgcaccg ccgacacatc tccctgaccg cgttccatgc
     2101 ggtcgagcgc gatcagcacg cccgccggtt ccgcgccgtt ggcgcgaatc agtgcgaccg
     2161 attcgcgcac cgacgttccg gctgtgatga catcgtcgac gatcatcacg cggccacgca
     2221 gcttcgcacc gacgacgctt ccgccttcac catggtcctt cgcttccttg cgattgaatg
     2281 catagggcag attgtgaccg cgtgcggcaa gcgcgatcgc cgtcgccgac gcaagggcaa
     2341 tgcccttgta tgccggtccg aagagctgat cgcaatcgac gccggattcg agcaacgcct
     2401 gcgcgtagaa ctcgccgagc cgaccgaggc tcgcaccgtc acagaacaaa cctgcgttga
     2461 aaaagtacgg gcttttgcgc cccgccttcg tcatgaactc gccaaagcga agcacgtcat
     2521 gtgccaatgc aaactgcagg aattcccgtc gaaaatcgct catttgtcgt gaacacgctg
     2581 aaacgttcgc tttgagtata gtccgcgcgt cgggcggacg ctcgggtaaa ctcggccgtt
     2641 tttca
//
LOCUS       DASSVX010000092         9376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1119471, whole genome shotgun sequence.
ACCESSION   DASSVX010000092 DASSVX010000000
VERSION     DASSVX010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9376
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119471"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..527)
                     /locus_tag="VFO53_04190"
     CDS             complement(105..527)
                     /locus_tag="VFO53_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HET8875509.1"
                     /translation="MFTPSRDEARRFLIDAWRKYRAGEPLSGLEQTAAQLIAMHPEYH
                     PVLETPDTHVDRDYRPEQGDINPFLHLSLHLAVAEQLAIDQPPGIVAHYARLRDARGD
                     AHEALHAIVECLGEVIWQAQRTGGSPDGAAYLACLERQ"
     gene            complement(532..1224)
                     /gene="nth"
                     /locus_tag="VFO53_04195"
     CDS             complement(532..1224)
                     /gene="nth"
                     /locus_tag="VFO53_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET8875510.1"
                     /translation="MNPQKRRQIFERLRAANPSPRSELDYRTPFELLVAVVLSAQATD
                     KGVNKATARLFPVANTPAAFVQLGVDGLIPYIGSIGLFNTKAKNVIALAQQLLDQHGG
                     EVPRHRDALEKLPGVGRKTANVVLNVAFGEPTIAVDTHIFRVANRTRLAVGKDPEAVE
                     RRLVKFTPEEFRPNAHHWLILHGRYVCVARVPKCPQCVIADLCEYPSKTRALPGTSAR
                     ALPLEPPAAAED"
     gene            complement(1240..1836)
                     /locus_tag="VFO53_04200"
     CDS             complement(1240..1836)
                     /locus_tag="VFO53_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HET8875511.1"
                     /translation="MDALSELAARIDGLLPQTQCRRCGYDGCGPYAAAIAAGDADVDR
                     CPPGGPDTRDALARLLGRPAPPLDTSRGVPGPLRVARIDEEACIGCTLCIAACPVDAI
                     IGAQKRMHAVLTSLCSGCELCVAPCPVDCIEMVPAGRDWTATDATAARERFDRRRERL
                     AATSTGARRDAEVRKRKAAIADAFERARARRRAALAQT"
     gene            1855..2823
                     /locus_tag="VFO53_04205"
     CDS             1855..2823
                     /locus_tag="VFO53_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET8875512.1"
                     /translation="MGATHSHHGSLHAHDGDAGDHHLPAGTGALRALAAALALTAGYA
                     VVEAVGGWLAGSLALLSDAGHMATDAAALGLALFAQWIARRPPSPRASYGYARAEVLA
                     AFVNALALLLLVGFIAFEAIRRIVTPAPVAGGTVLGVATVGLLVNAVCAWVLSRASSS
                     AGAHSALLHVLSDALGSIAAIVAGAVILATGWTPIDPLLSLLTAVLILRATWRLLAQT
                     TGVLMEGVPAHLDYREIGGALTAIAGVRGVHDLHVWLMSTRQAALSAHVTIDSGDAWP
                     PILDAARHMLAERFQIDHVTLQPSWPPESVYADRRVIPIVPADGSA"
     gene            complement(2843..3823)
                     /gene="corA"
                     /locus_tag="VFO53_04210"
     CDS             complement(2843..3823)
                     /gene="corA"
                     /locus_tag="VFO53_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET8875513.1"
                     /translation="MLVSCVAYEHGRKIADIPVADISDYVHRPNYLIWVALFEPTVEE
                     LDEMAQEFGLHELAVEDARKGHQRPKIEEYGNSLFAVLHPAEMVKGGDGGDELNIGEI
                     DIFVGPNYVLTVRHRTQIGFAAVRARTEREPELLSHGAGFVFYTLLDNVVDRYFPVLD
                     QLETRLESAEEQLFGSAATRRKIEELYGLKRELMALKHAVAPLMEAVGKLFGGRVPQM
                     CLGTQEYFRDVYDHLARINGTIESTREMLTTAIQVNLALIGISDNEVTKRLAAYGALI
                     TVPTLIAGIYGMNFKNMPELNWSVGYPLAIFCMVAIDAILFLRFRRAKWV"
     gene            3932..5176
                     /gene="pncB"
                     /locus_tag="VFO53_04215"
     CDS             3932..5176
                     /gene="pncB"
                     /locus_tag="VFO53_04215"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET8875514.1"
                     /translation="MAGFARRRASATRRQTKRAMIITSLLDTDLYKFTMMQVVLHHFP
                     AAQAEYRFKCRNGGVDLQPYIGEIRREIAALCRLRFTRRELDYLRSWRFLKSDFVDLL
                     GLFRLDERFIHVRALPDSPGEIDIAIKGPWLHTIMFEVPVLAIVSEVYNRNMHPQPDY
                     GEGERRLARKIEQLNSVPDPAFRIADYGTRRRFARDWQERVIVALRDGIREKFVGTSN
                     VHFALEHNLTPLGTMAHEYLQACQAVGPRLRDSQVFAFNTWAREYRGDLGIALSDVFG
                     MDAFLRDFDLFFCKLFDGVRHDSGDPFEWGEKLIAHYQRMRIDPRSKAMVFSDALDVP
                     LALRLFEYFRGRCQTAFGIGTNLTNDLGYEPLAIVIKMTRCNGQPVAKISDEPSKTMD
                     YDPSYVAYVREVFQVPAINPEK"
     gene            complement(5235..5606)
                     /locus_tag="VFO53_04220"
     CDS             complement(5235..5606)
                     /locus_tag="VFO53_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875515.1"
                     /translation="MKTTIGALALLAAGLLVTGPAISGSGDTRLADGGWNSRYGPPRD
                     ERSGSRGNWAVGTFHGQNGANGNDETVTIRRDGSVELRTRGEAPKYGTFEGETLTLGS
                     RISKVERARGGIVIDGAYYRR"
     gene            complement(5654..6187)
                     /locus_tag="VFO53_04225"
     CDS             complement(5654..6187)
                     /locus_tag="VFO53_04225"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HET8875516.1"
                     /translation="MPVVGGTTLEARDVDIATLDVGAIVNAANSSLLGGGGVDGAIHR
                     AAGPQLLAECRTLGGCPTGEARVTGGYRLPARYVIHTVGPVWQGGAHGEPALLAACYR
                     NSLALARDLRVASIAFPAISCGVYGYPLELATAIAIREVAQTVAAMPALEEVVFACFG
                     EHALAAYRIALTACEAA"
     gene            6343..8049
                     /locus_tag="VFO53_04230"
     CDS             6343..8049
                     /locus_tag="VFO53_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET8875517.1"
                     /translation="MVADGLIAQADATQIGRTRTQRFDHPLELIAEQKIRSQRPPGKL
                     LGLDYLAEWLAGKLGVPYQHIDPLKIDLGAVTATMSNAYAERYRILPVAIDRRTLTVA
                     TGEPFVRSWADELERILKLEIRLVLANPADVRRYIGEFFNLARSMKRAQENSRGDFAI
                     ARNFEQLVELGRHGHLDANDQHVVHIVDWLWQYAFDQRASDIHIEPRRDVGLVRFRID
                     GVLHQVYAIPTSVLNAVTSRIKLLARMEIVEKRRPQDGRIKTVAPDGQEIELRISTMP
                     TAFGEKIVMRIFSPEVLVRDFQDLGFTVDDRSRWEAMTGEPHGIILVTGPTGSGKTTT
                     LYSTLKGLATTEVNVCTIEDPIEMIEPAFNQMQVQSSIGVDFASGVRTLLRQDPDIIM
                     VGEVRDRDTGDMAVQAALTGHLVLSTLHTNDAPTAVTRMLDLGIPAYLINSTLLGVMA
                     QRLVRTLCPHCKAPADPPDEATWEAITSPWRAEKPATTMAARGCLECRMTGYLGRIGL
                     YEIMLMTPALRRLMTQDADDRAIREQAFRDGMKPLRVSGATKVAAGLTTADEVIKVAP
                     LA"
     gene            complement(8101..8391)
                     /locus_tag="VFO53_04235"
     CDS             complement(8101..8391)
                     /locus_tag="VFO53_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875518.1"
                     /translation="MELYWVPIGFAVVVLLLVLLLARLVGNAKRNEIRKRLGLPVNSR
                     LTPEQASAFQAFENTDMKLKKSFPTLSDTQRQAMARDVLRDKGMLPKRKKTR"
     gene            complement(8491..8577)
                     /locus_tag="VFO53_04240"
     tRNA            complement(8491..8577)
                     /locus_tag="VFO53_04240"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8541..8543),aa:Ser,seq:tga)
     gene            8733..>9376
                     /locus_tag="VFO53_04245"
     CDS             8733..>9376
                     /locus_tag="VFO53_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET8875519.1"
                     /translation="MEPRSQTVFNPGTAPAVSVAESQNRVLRNTYWLLALSMLPTVAG
                     AWVGMQLNFLSLFAASPIMAPLLMFGVMIGALFGVTALRNSAWGVPALFGFTFIAGLM
                     LAPILSVAIGFRNGGQLIGLAGGMTAAIFFAMAGIATVSKRDFSFMGKFLFIGLVLLI
                     VASLANIFLQIPALSVTVSAIAVLLFSAYLLHDVSRIVRGGETNYITATLSLFL"
BASE COUNT         1463 a         3137 c         3149 g         1627 t
ORIGIN      
        1 cagagacgca tttcccggta cagcgcagaa ttttggctga ccgcgtcggg aaatgcgtct
       61 ctgaccctat ttattggtac cgggaaatgc gtctctgacc ctatttattg ccgctcgagg
      121 caggcgagat aggccgcgcc gtccgggctg ccgcccgtgc gctgcgcctg ccagatcact
      181 tccccgaggc attcgacgat cgcgtgcaac gcctcgtgcg cgtcgccgcg cgcgtcgcgc
      241 aaccgcgcgt aatgcgcaac gatgccgggc ggctggtcga tcgccagttg ctcggcgacc
      301 gcgaggtgca gcgacaggtg caggaacgga ttgatgtcgc cctgctcggg gcggtaatcg
      361 cgatcgacgt gcgtgtcggg ggtttcgagc accgggtggt actcggggtg catcgcgatc
      421 aattgcgccg ccgtctgctc gagtcccgac agcggctcgc ccgcgcggta cttgcgccac
      481 gcgtcgatca gaaagcggcg cgcctcgtcg cggcttggcg tgaacacgcg gtcaatcctc
      541 cgccgccgcg ggcggctcga gcggcaacgc gcgcgcgctc gtgcccggta gcgcgcgcgt
      601 cttggacgga tactcgcaaa ggtcggcgat cacgcattgc gggcatttcg gcacgcgtgc
      661 gacacacacg tagcgcccgt gcaggatcag ccagtggtgc gcgttcggcc gaaactcctc
      721 cggggtgaat ttgacgagcc ggcgttcgac cgcctcggga tccttgccga cggcgagccg
      781 cgtgcggttc gccacgcgga aaatgtgcgt gtcgaccgcg atcgtcggct cgccgaacgc
      841 gacgttgagc accacattgg ccgtcttgcg cccgacgccg ggcagctttt ccagcgcgtc
      901 gcgatggcgc ggcacttcgc ccccgtgctg gtcgagaagc tgctgcgcga gcgcaatcac
      961 gttcttcgcc ttcgtgttga agagcccgat cgagccgatg tacgggatca gcccgtcgac
     1021 gccgagttgc acgaatgcgg ccggcgtgtt ggcgaccggg aaaagccgcg ccgtggcctt
     1081 gttgaccccc ttgtcggtag cctgcgccga cagcaccacg gccaccagca gctcgaacgg
     1141 cgtgcggtag tcgagctcgc tgcgcgggct cggattggcg gcgcgcaggc gctcgaagat
     1201 ctgccttcgc ttttgcggat tcatcgcttc tcgtgcgggc tacgtctgcg caagcgccgc
     1261 gcggcgccgg gcgcgggcgc gttcgaacgc gtcggcaatc gcggccttgc gtttgcgcac
     1321 ttcggcgtcg cgcctcgcgc cggtgcttgt cgccgccagg cgctcgcggc gccgatcgaa
     1381 acgctcgcgc gcggccgttg catcggtcgc cgtccagtcg cgtccggcag gaaccatttc
     1441 gatgcagtcc accgggcagg gcgcaacgca cagttcgcag ccgctgcaaa gcgatgtcag
     1501 cacggcgtgc atccgcttct gtgcgccgat gatcgcgtcg accgggcagg cggcgatgca
     1561 aagcgtgcag ccgatgcacg cttcctcgtc gatgcgcgcg acgcgcagcg gacccggtac
     1621 gccgcgcgag gtgtcgagcg gtggggccgg gcggccgagc aggcgtgcga gcgcgtcgcg
     1681 ggtgtcgggt ccgccgggcg ggcagcggtc gacgtcggca tcgcccgcgg caatcgcagc
     1741 ggcgtacgga ccgcatccgt cgtacccgca gcggcggcac tgcgtctgcg gcagcaggcc
     1801 gtcgatgcga gctgcaagtt cggacaacgc atccatggtg ctattttagc gacgatgggc
     1861 gcaacgcatt cgcatcacgg ctcgctgcac gcgcatgatg gcgatgcggg cgatcaccat
     1921 ctgcccgccg ggacgggggc gttgcgcgca cttgccgcgg cgctggcgct gaccgccggc
     1981 tacgcggtcg tcgaggccgt gggcggctgg ctcgccgggt cgctcgcgct tctgtccgac
     2041 gccgggcaca tggcgaccga cgccgccgcg ctcggtctcg cgctcttcgc gcagtggatt
     2101 gcgcgccggc cgccgtcgcc gcgcgcgtcg tacggctacg cacgcgccga ggtgctcgcc
     2161 gcgttcgtca atgcgctggc gctgctgctg ctcgtcggct tcatcgcgtt cgaggcgatc
     2221 cgacgcatcg tgacgcctgc gcccgttgcc ggcggcaccg tgctcggcgt cgccaccgtg
     2281 gggctgctcg tcaacgcagt gtgcgcctgg gtgctgtcgc gcgcgtcgtc gtcggccggt
     2341 gcgcacagcg cgttgctgca cgtgctgtcc gacgcgctcg gctcgatcgc cgcgatcgtc
     2401 gccggcgcgg tgatcctcgc gaccggctgg acgccgatcg atccgctgct gtcgctgctg
     2461 acggccgtac tgatcctgcg tgcgacgtgg cgcctgctcg cgcagacgac cggcgtgctg
     2521 atggagggcg tgccggcgca cctcgattac cgcgagatcg gcggcgcgct gactgccatc
     2581 gcgggggtgc ggggcgtgca cgacctgcat gtgtggttga tgagcacccg gcaggcggcg
     2641 ctgtcggccc acgtgacgat cgacagcggc gatgcgtggc caccgatact ggacgccgcg
     2701 cgccacatgc tcgccgagcg cttccagatc gaccacgtca cgctgcagcc gtcgtggccg
     2761 cccgaatcgg tttacgcgga ccggcgcgtg attccgatcg ttcccgcgga tggaagcgca
     2821 tgagcaacgc cgccggggtc aatcagaccc acttggcgcg ccggaagcgc aggaacagga
     2881 tcgcgtcgat cgcgaccatg cagaagatcg cgagcggata accgaccgac cagttgagct
     2941 cgggcatgtt cttgaaattc atgccgtaga tccccgcgat cagcgtcggc accgtgatca
     3001 gcgcgccgta cgcggcaagc cgcttcgtca cctcgttgtc cgagatcccg atcagcgcga
     3061 ggttgacctg gatcgccgtc gtcagcattt cgcgcgtgct ttcgatcgtc ccgttgatgc
     3121 gcgcgagatg gtcgtagacg tcgcggaaat attcctgcgt gccgagacac atttgcggga
     3181 cgcgcccgcc gaacagcttg ccgaccgcct ccatcagcgg cgcgaccgcg tgcttcagcg
     3241 ccatcagctc gcgcttgagc ccgtacagct cctcgatctt gcgccgcgtt gcggcacttc
     3301 cgaagagctg ctcctcggcg ctttcgaggc gcgtctcgag ctggtcgagc accgggaaat
     3361 agcggtcgac cacgttgtcg agcagcgtgt agaacacgaa gcccgcgccg tgactcagaa
     3421 gctcgggctc gcgctcggtg cgcgcgcgta ccgcggcaaa gccgatctgc gtgcggtggc
     3481 ggaccgtgag cacgtagttg gggccgacga agatatcgat ttcgccgatg ttgagctcgt
     3541 cgccgccgtc cccgcccttg accatttctg cggggtgcag cacggcaaag agcgagttgc
     3601 cgtactcctc gatcttgggt cgttgatgac ccttgcgcgc atcctcgacg gcgagctcgt
     3661 gcaggccgaa ttcctgcgcc atctcgtcga gctcctcgac cgtgggctcg aacagcgcca
     3721 cccagatcag gtaattcggc cgatgcacgt aatcgctgat gtcggcgacc ggaatgtcgg
     3781 cgatcttgcg gccgtgttcg taagcgacgc agctgacgag catgatgcgg ggagaggttg
     3841 actggccgcc atcatagcgg cggcggcggc ggcgggccag cgctcgcacg acgatgcggc
     3901 cgcgggcgcg tcctgcgcac cacggggtaa gatcgccggt ttcgcccgcc gtcgtgcatc
     3961 cgccacgcgg cggcaaacga agcgcgccat gatcatcacg tcgctgctcg acaccgatct
     4021 ctacaaattc acgatgatgc aggtcgtgct gcatcatttc ccggccgcgc aggccgagta
     4081 tcgtttcaaa tgccgcaacg gcggcgtcga cctgcagccg tacatcggcg agatccggcg
     4141 cgaaatcgcc gcactttgcc ggctgcgctt cacgcgtcga gagctcgatt acctgcgaag
     4201 ctggcgcttc ctcaagagcg atttcgtcga cctcctcggg ctcttccggc tcgacgagcg
     4261 cttcattcac gtccgcgcgt tgcccgacag cccgggcgag atcgatatcg cgatcaaggg
     4321 gccgtggctg cacacgatca tgttcgaggt gccggtgctc gcgatcgtgt ccgaggtgta
     4381 caaccgcaac atgcatccgc agccggatta cggcgagggc gaacggcgcc ttgcgcgcaa
     4441 gatcgagcag ctcaattcgg tgccggatcc ggcgtttcgc atcgccgact acggaacgcg
     4501 gcggcgcttc gcgcgcgatt ggcaggagcg cgtgatcgtc gcattgcgcg acggcatccg
     4561 cgagaagttc gtcggaacga gcaatgtcca tttcgcgctc gagcacaacc tgacgccgct
     4621 cggcacgatg gcgcacgaat acctgcaggc gtgccaggcg gtgggcccac ggctgcgcga
     4681 ttcgcaggtg ttcgccttca acacgtgggc gcgcgagtac cgcggcgatc tcgggatcgc
     4741 gctgtccgac gtgttcggca tggacgcgtt cctgcgcgac ttcgatctgt tcttctgcaa
     4801 gctgttcgac ggcgtgcgcc acgattccgg cgatccgttc gaatgggggg aaaagctgat
     4861 tgcgcactat cagcgcatgc gcatcgaccc gcgcagcaag gcgatggtgt tctcggatgc
     4921 cctcgacgtg ccgctcgcgc tgcggctgtt cgaatatttc cgcggccgct gccagaccgc
     4981 gttcggcatc ggcaccaacc tcaccaatga cctcggctac gagccgctcg cgatcgtcat
     5041 caagatgacg cgctgcaacg gccagccggt ggcgaagatc agcgacgagc cgtcgaagac
     5101 gatggactac gatccgtcct acgtggcgta cgtgcgcgag gtgtttcagg ttccggcgat
     5161 caatccggaa aagtagcgcg aaccgcttta aggccgcgct cgcaacatcg gcaccggtca
     5221 cacgttcgag gccgtcagcg gcggtagtac gcgccgtcga tcacgatgcc gccgcgcgca
     5281 cgctccacct tgctgatgcg cgaccccagc gtcagtgttt cgccttcgaa cgtcccgtac
     5341 ttcggcgctt ccccgcgcgt gcggagctcg acgctgccgt cgcggcggat cgtgaccgtc
     5401 tcgtcgttgc cgttcgcgcc gttctggccg tggaacgtgc cgacggccca gtttcccctc
     5461 gatccgctgc gctcgtcgcg gggcgggccg taccgcgagt tccatccgcc gtcggcgagc
     5521 cgcgtgtcgc cggaccccga gatcgccggt ccggtaacga gcaggcctgc ggcgagcagg
     5581 gcgagtgcac cgatggtcgt cttcatggcg atcctcctca tgggcgattg gagcaaggtg
     5641 cgcgcgcgtt ggttcaggcc gcttcgcacg cggtcagcgc gatccggtat gcggcgagcg
     5701 cgtgctcgcc gaagcaggcg aatacgactt cttcgagcgc cggcatcgcg gcgaccgttt
     5761 gcgcaacctc gcgtatcgcg atcgcggtcg cgagctcgag cgggtaaccg taaacgccgc
     5821 aactgatggc gggaaacgcg atcgatgcga cgcgaagatc gcgcgccagc gcgagcgaat
     5881 tgcgatagca cgcggcgagg agggcgggct cgccgtgcgc accgccttgc cacaccgggc
     5941 cgaccgtatg aatcacgtac ctcgccggca ggcgatagcc gccggtcacc cgcgcttcac
     6001 cggtcgggca gccgccgagc gtgcggcact cggccagcaa ctgcggtccc gccgcgcggt
     6061 ggatcgcgcc gtcgacgcca cccccgccaa ggagcgacga attcgcggca ttgacgatgg
     6121 cacccacgtc gagcgtcgct atatcgacgt cacgggcttc gagggtggtg ccgccgacga
     6181 ccggcatgga aaactccgca gaattgcgcg aacgacagtg taaagtcccg gtgcatggca
     6241 acgccaatct ccgaaaccaa gccgggcgaa gcgccgcccg tgccgggtgg agcgagggcg
     6301 gccgccgatc gccggcttcg gctcgacgac atcctgaagc tgatggtcgc cgacggcctg
     6361 atcgcacagg ccgacgccac gcagatcggg cgcacgcgca cgcagcgttt cgatcatccg
     6421 ctcgagctca tcgccgagca gaagatccgg tcgcagcgac cgccggggaa actgctgggc
     6481 ctcgactacc tcgccgagtg gctggccggg aaactcggcg tgccttatca gcacatcgac
     6541 ccgctgaaga tcgatctcgg cgcggtgacc gcgacgatgt cgaatgcgta cgccgagcgc
     6601 tatcggatcc tgccggttgc catcgaccgg cgcacgctga cggttgcgac gggcgagccg
     6661 ttcgtccgca gctgggccga cgagctcgaa cgcatcctga agctcgagat ccgtctcgtg
     6721 ctcgccaacc cggccgacgt gcgccgctac atcggcgagt tcttcaacct cgcgcgctcg
     6781 atgaagcgcg cacaggagaa ctcgcgcggc gacttcgcga tcgcgcgcaa cttcgagcag
     6841 ctcgtcgagc tcggccggca cggccatctc gacgccaacg accagcacgt cgtccatatc
     6901 gtcgactggc tgtggcaata cgcgttcgac cagcgcgcgt cggacatcca tatcgagccg
     6961 cggcgcgacg ttggcctcgt gcgctttcgc atcgacggcg tgctgcacca ggtctacgcg
     7021 attccgacct ccgtgctgaa tgccgtgaca tcgcgcatca agctgctcgc ccggatggag
     7081 atcgtcgaga agcggcggcc gcaggatgga cgcatcaaga cggtcgcacc cgacggccag
     7141 gagatcgagc tgcgcatctc gacgatgccg acggcattcg gcgagaagat cgtgatgcgc
     7201 attttcagcc cggaagtgct ggtgcgcgat ttccaggatc tcggcttcac cgtcgacgac
     7261 cgctcgcgct gggaagcgat gaccggcgag ccgcacggga tcatcctcgt caccggaccg
     7321 accggctcgg gaaagacgac gacgctctat tcgacgctga agggccttgc gacgaccgaa
     7381 gtgaacgtct gcacgatcga ggacccgatc gagatgatcg agccggcgtt caaccagatg
     7441 caggtgcagt cgtcgatcgg cgtcgatttc gcatccggcg tgcgcacgct gctgcgccag
     7501 gacccggaca tcatcatggt cggggaagtg cgcgaccgcg acaccggcga catggcggtg
     7561 caggccgcgc ttaccggaca cctcgtgctg tcgacgctgc ataccaacga cgcgccgacg
     7621 gcggtgacgc gcatgctcga cctcggcatc cccgcctatc tcatcaactc gacgctcctc
     7681 ggcgtgatgg cgcagcggct cgtgcgcaca ctgtgcccgc attgcaaggc gcccgccgat
     7741 cccccggacg aagcgacgtg ggaggcaatc acgtcgccgt ggcgggcgga gaagccggcg
     7801 acgacgatgg cggcacgcgg ttgcctcgaa tgccggatga cgggctatct cggccgcatc
     7861 ggcctctacg agatcatgct gatgacgccc gcactgcgcc ggctgatgac gcaggacgcg
     7921 gacgaccgcg cgattcgcga gcaggcgttt cgcgacggca tgaagccgtt gcgcgtatcc
     7981 ggggcgacga aggttgcggc gggtctgacg accgccgacg aggtgatcaa ggtggcgccg
     8041 ctcgcctgac cgcggcgccg tcgaaggcga ttggcgcgaa gctttccgcg ccggcggcgg
     8101 tcagcgcgtc ttcttgcgct tcggcagcat gcccttgtcg cgcagcacgt cgcgcgccat
     8161 cgcctgccgt tgcgtgtcgg atagtgtcgg aaacgacttc ttgagcttca tgtccgtgtt
     8221 ctcgaatgcc tggaacgccg acgcctgttc gggcgtgagc cgcgagttca ccggcaaccc
     8281 gagcctcttg cggatttcgt tccgcttcgc gttaccgacg agccgggcga gcaacagcac
     8341 gagcagaagc acgacgactg cgaagccgat cggcacccag tacagttcca cgattcgtct
     8401 cctggagcaa actcacgaat ggtacgtgag gcgcgaatgc aatcgtcatg ccactggact
     8461 ggcgcggcgc cggtagcgac cgccggggag cggatggggt gggattcgaa cccacgaatc
     8521 gcttgcacga ttgccggatt tcaagtccgg tgcattcaac cgctctgcca cccatccatg
     8581 ctcggcgctt gattccggcg gggggcgggc gcatcttagc actcgcggga acggctggca
     8641 taagggaaac ccggatcgga tcgcgcgccg tttctgctag tctagcaacg cttgacgtac
     8701 atcccgggcc gctggccctc tttggaggct tcatggaacc caggtcgcaa acggttttca
     8761 accccggtac cgcgccggca gtcagcgttg ccgagagcca gaaccgggtg cttcgcaaca
     8821 cctattggct gcttgcgctg tcgatgctgc ccacggtcgc cggtgcgtgg gtcggcatgc
     8881 agttgaattt tctgagcctg ttcgcggcgt cgcccatcat ggcgccactg ctgatgttcg
     8941 gcgtgatgat cggcgcgctt ttcggcgtca ccgcgcttcg caacagcgcg tggggcgtgc
     9001 ccgcgctctt cggcttcacg ttcatcgccg ggctcatgct ggcgccgatc ctgtcggttg
     9061 ccatcggctt tcgcaatggc ggccagctga tcgggctcgc cggcgggatg accgccgcga
     9121 tcttcttcgc gatggcgggc attgccaccg tgtcgaagcg cgatttctcg ttcatgggca
     9181 agttcctgtt catcgggctc gtgctgctga tcgtcgcgtc gctcgcgaac atcttcctgc
     9241 agatacctgc gttgtcggtc acggtctccg cgattgccgt gctgctgttc tccgcgtacc
     9301 tgctgcatga cgtcagccgg atcgtgcgcg gtggcgaaac gaactacatc accgccacgc
     9361 tgagcctgtt cctcga
//
LOCUS       DASSVX010000093        17407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1386361, whole genome shotgun sequence.
ACCESSION   DASSVX010000093 DASSVX010000000
VERSION     DASSVX010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 17407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17407
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386361"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VFO53_04250"
     CDS             <1..569
                     /locus_tag="VFO53_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET8875520.1"
                     /translation="ELGGSLRIEPVTIALRYNAIDARFRSTFDAASPDNSEADENGGI
                     VVRAGNRMPGIPVQTAKLRLDWDVTPGVAIGASLVAAAGQYANGDENNADRSGRVPGY
                     FVMHLDAQYVMTPRVTLFAKIDNLFDRRYANFGLLGSNVFTGPERSFGPAVGMAPVNE
                     QFRALGSPRGVFVGVRLTLDAPAHGVRD"
     gene            complement(501..1364)
                     /locus_tag="VFO53_04255"
     CDS             complement(501..1364)
                     /locus_tag="VFO53_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HET8875521.1"
                     /translation="MRGAVIGLILALGLAPVALAQDENPDDAAVWRKLHATVFADAPI
                     ERGDAVVELDTPKRAVDATIVPVAIRTRLTQSTGHFIETIWLIVDNNPSPVAAIFHFT
                     PQSGRADVETRIRIEQYTHVRAIARTNDGRLFMATSYVKASGGCTAPRGSDPASALAT
                     LGRMRMKVDGTPVPGRPILAQLMISHPNDSGLAMDPMARTYAAPHFVRTVEVTYAGKP
                     VMRADVDFSISENPNFRFYFTPGNGGQLAARIVDNQDQAFSTAISVESIADAMRRRIE
                     GQPDAHENAAR"
     gene            complement(1412..1750)
                     /locus_tag="VFO53_04260"
     CDS             complement(1412..1750)
                     /locus_tag="VFO53_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875522.1"
                     /translation="MRYLLLLAICGATLASANDFPTRARVDFVLTCMQESKATPQEAM
                     YKCSCAIDAIADKVPYREWVDLSTIANGITIAGERGGAMRDIKDGRKLIAAYRKLQDN
                     ARKQCFIRSE"
     gene            complement(1769..2674)
                     /locus_tag="VFO53_04265"
     CDS             complement(1769..2674)
                     /locus_tag="VFO53_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HET8875523.1"
                     /translation="MTEEHCRAMPVAEKRAVPTAAFRRLAAVLAALLVATASASALAD
                     PARTAFRPCIDPSNLPFANEKGEGFENRIADLFAQKLGIPVQSYAFPQRMNFIRNTLR
                     YRLPGEDFRCDIVMSVPASYDQAWPTAPYYRSTYALVYRKGHGLDQVQSGSDLLALPA
                     DVRGKLTIGIYDRSPAAYWLVKHGMEAQTKPFPMMSPDPDRYPGEIIDKDLAQGRIDA
                     AIVWGPIAGYFAKRVHDVELVVIPLQSEPGVKFDYAIAMGVRQGDAEWRATVDKLIAE
                     NKPAITAILREYDVPLVDERGESIK"
     gene            complement(2671..3087)
                     /locus_tag="VFO53_04270"
     CDS             complement(2671..3087)
                     /locus_tag="VFO53_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET8875524.1"
                     /translation="MRNMIGVLALLLFAAQGPVSAQDKAPAPSSDKPLYKVVDGYKVD
                     SATMNGFRAWRAAACDRCHGANQEGLVGPSLIESLKTMSKEDFIKTVRDGRLDKGMVS
                     WKDSKMVMENIDSLYAYLKGRSNGDITRAHVEELKQ"
     gene            complement(3281..5095)
                     /locus_tag="VFO53_04275"
     CDS             complement(3281..5095)
                     /locus_tag="VFO53_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HET8875525.1"
                     /translation="MKIVDKPWFQTVLAMAWLLAFPGSASANADLEKMIANPDNWAMQ
                     AGDMYNHRYSNLSQINAKNVGKMQVAWMFSTGVLRGHEGSPLVMNGTMYLHTPFPNKV
                     FAIDLDTQKIKWRYEPKQDAAVIPQMCCDTVYRGLAYAENKIFLQQADSMLVALDANT
                     GKVLWSVKNGDPKVGAVNTNAPHVFKDKVITGISGGEWGVRGYITAYDINSGKQVWRA
                     YSMGPDEEMLVNPEKTMTWTDGAMKPVGKDSSLKTWQGEQWKNGGGTTWGWYSYDKGL
                     NLLYYGSGNPSTWNPAQRPGDNKWSMAIWARDVDTGEAKWVYQMTPWDQWDYDGINEM
                     ILADINVKGKPTKALVHFDRNGFGYTLDRATGALLVAEKYDPAVNWATRVDMKTGRPE
                     VVKKYAPGENGTDVNNKGICPAALGSKDEQPAAFDPKTKRFYVPTNHVCMDYEPFKVE
                     YTAGQPYVGATLSMFPAPGGTNMGNFIAWDAGTGKIVQTKAEKFSVWSGPLATAGGIV
                     CYGTLEGYLKCVDANNINKELFSFKTPSGIIGNVFTYQHKGKQYMGVFSGIGGWAGIG
                     MAAGLEKDQEGLGAVGGYKELRDYTNLGGSLTVFALPM"
     gene            5317..6279
                     /locus_tag="VFO53_04280"
     CDS             5317..6279
                     /locus_tag="VFO53_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8875526.1"
                     /translation="MSSAAGALARFACLLLALACASAHASAPLAVAPGVFVLMGRDGE
                     ISPDNLGRIANIAFIVGPRGVVVVDSGVSFRQGEAIIAAVRRVTRRPIRLVVMTHASR
                     EVVFGAAAFQAHGIPVWMHAAAAALMASRCDACLTGLAETLGAEAMAGSRVVAPDRLV
                     AGTLLLDVIGRPLMVVAPAQGSAPGAIAVFDVATRTLIAGTLVSIDRVPDMHDASGPW
                     RGALAMLAATHCVHLIPAYGRVGSCADIDGIERYFAALEQRVRELLAADVGLAELPAR
                     CDLPEFSAWGRYGALHVGNASREYLRMERASFKDRDWKPTGVKD"
     gene            6317..7426
                     /locus_tag="VFO53_04285"
     CDS             6317..7426
                     /locus_tag="VFO53_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HET8875527.1"
                     /translation="MDVRAAVAYKAGQPLVIESVQLAGPKAGEVLVEIKATGICHTDE
                     FTRSGADPEGLFPAILGHEGAGIVVDVGPGVSSVAKGDHVIPLYTPECRQCKSCLSRK
                     TNLCTAIRATQGRGVMPDGTSRFSLRGEPIHHYMGCSTFANFTVLPEIAVAKIRQDAP
                     FEKVCYIGCGVTTGIGAVINTAKVEAGANVVVFGLGGIGLNVVQGARMVGANMIIGVD
                     LNPAREALARKFGMTHFVNPKDVKGDLVAHLVELTGGGADYSFECIGNVDVMRQALEC
                     CHRGWGVAVIIGVAGSGQEIRTRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMDGK
                     INIDDLITHVLPLDRINDGFDLMHAGKSIRTVVTY"
     gene            7458..8036
                     /gene="gfa"
                     /locus_tag="VFO53_04290"
     CDS             7458..8036
                     /gene="gfa"
                     /locus_tag="VFO53_04290"
                     /EC_number="4.4.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051907 - S-(hydroxymethyl)glutathione
                     synthase activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione synthase"
                     /protein_id="HET8875528.1"
                     /translation="MPTPSIHPAVDHGIKPAAANFSGGTLYCNCSEGKVAVSIKGQTA
                     HNHVCGCTKCWKPSGALFSQVAVVSRDKVSVSANGNKLQVVDPSATIQRHACKSCGVH
                     MYGRIENTGHPLYGLDFVHTELSPEQGWSPPEFAAFVSSIIESGANPANMGAVRSRLK
                     EIGLEPYDCLSPPIMDLIATHTAKAKGVLASA"
     gene            8123..8992
                     /gene="fghA"
                     /locus_tag="VFO53_04295"
     CDS             8123..8992
                     /gene="fghA"
                     /locus_tag="VFO53_04295"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HET8875529.1"
                     /translation="MSQAPRPLSQNRSFGGVQYVYAHAAETTRCDMRFGVFLPPQATS
                     RAVPVLYWLSGLTCTEENFIVKAGAQRIASELGLAIVVPDTSPRGLGIPGEAESYDFG
                     LGAGFYVDATEAPWSRGYRMYSYVTKELPALVEEHLPVDAERVGIFGHSMGGHGALTI
                     ALRDPDRYRTVSAFAPIASPMHSPWGEKAFTGYLGADRDRWRDYDATALLDARGWPGP
                     PLFVDQGTDDPFLERELKPGLLKGACERAGVELELRMQRGYDHSYHFVATFIEDHLRF
                     HASRLSSAPAGGS"
     gene            9001..9774
                     /locus_tag="VFO53_04300"
     CDS             9001..9774
                     /locus_tag="VFO53_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET8875530.1"
                     /translation="MRRAMVAAFVALAGASSPDAADFTADQVRQRLANASDGKSADFT
                     GKDLSDLDLGHIDFRRADLSGANLFASRLVSSNLAGAKLAGANLNGAWLMGASFAGAD
                     LSGSTMLGLVILGGDVKEKPNFAGANLSGVRMIADLPGADLHGANLRRARLGVDMRNQ
                     GMGQMRTDLSNANLSGADLRDADLSRSVMMFADLRGADLRGANLFGVKLAGANLTGAQ
                     IADADFTEADLDATVFTGVRDRDAAKGLDKAINGERAIR"
     gene            complement(9856..10296)
                     /locus_tag="VFO53_04305"
     CDS             complement(9856..10296)
                     /locus_tag="VFO53_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875531.1"
                     /translation="MNYSRLTAAAALAMGLTFPPLGSAQGQPAQRPDTGVKHPPTAQM
                     DAATPSGKTTTGEPDSAKHPPTSLMDRAVPDQKSPGAAEDEASPGSTQEETPPGTAQD
                     EAPQGSTQEETPPGTTQDENAPTTPPDEQSPQEGGQRPNPGIRV"
     gene            10571..10909
                     /locus_tag="VFO53_04310"
     CDS             10571..10909
                     /locus_tag="VFO53_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HET8875532.1"
                     /translation="MAAAKLIVIYPTPANLEAFEHAYSTGHIPMAAPIFEAAGATKVV
                     LTLVSAAASGPAPYHRIAEIHFPSMQALNACAASQGGRDALADAARISNGGAPIVLIA
                     TEDVVTFAGR"
     gene            complement(10944..12149)
                     /locus_tag="VFO53_04315"
     CDS             complement(10944..12149)
                     /locus_tag="VFO53_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8875533.1"
                     /translation="MTPLPRATSLLLNVAHAMDHMFLLIFATSVTAIAADFGFAHWED
                     LMPYGVGAFAMFGIGSLPAGRLGDLWGRRRMMLVFFFGMGACALLVAFTHSAWQLAAA
                     LALLGAFSAIYHPVGIPMLVQRAARPGLTIGFNGLAGNLGVAMSALVTGALLKYAGWQ
                     AAFVVPAVAAIGCGVLFARVAPAEGVAPARRAPSRAMLGKRALAHIFAVVTVASISGS
                     LLFNFTTNGNGELLRERLRGIVEDPAALGMLLAAVYVAGALAQVSVGLLLDRVPLKTL
                     YACIVVVQAPLFLLAANATGWVLYALMFAFMIVVFGAIPFTDAMIVRYVDDRMRSRVS
                     GMRLAVAFGVSSLAVWLLGPIVKARGFSALLLLMAAIAFATFCVVMLLPRTAMGGLGV
                     DDTAPQPGD"
     gene            complement(12270..13271)
                     /locus_tag="VFO53_04320"
     CDS             complement(12270..13271)
                     /locus_tag="VFO53_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HET8875534.1"
                     /translation="MPTLTVSELRDLAQNALAKAGASAAMASITARALADAEAQGLSS
                     HGMARVSQYATHLRNGRADGRAVAVIARERGGAILVDARCGLAFPACTLAVDEAIRRA
                     RDCGIAFAGVTNSHHFGVAAWHLEPAAAAGMVALAFANSPAAMPAAGGRRALFGTNPI
                     AAVFPRRDAPPLSIDLSLSEAARGKVMVAAKEGRPIPLGWALDRDGQPTTDAKAALAG
                     SMLAMGGTKGAMLALVVELLVTALTGAALGFEASSFFVDEGNRPRIGQAFLVIDPDAL
                     AGRDVFDERIETLIAAMQQDEGVRLPGARRAALAASASVHGVEISQALAEQLRALAQ"
     gene            complement(13291..14220)
                     /locus_tag="VFO53_04325"
     CDS             complement(13291..14220)
                     /locus_tag="VFO53_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HET8875535.1"
                     /translation="MRIVISEFMDSPAVAELASRFDVRYDKDLVDRRSELVADLHDAD
                     ALIVRNRTQVDAALVSAAPKLRVVGRLGVGLDNLDMDACATRGIAVIPATGANAQAVA
                     EYVIAAAMLLLRGAYFATTDVTAGRWPRARLSGGRELGGRTLGLIGFGGIGRLTGRLA
                     RALGMHTIAFDPQIPAAAPVWADDATRPCTLAELLGEADVVSLHVPLAAATRNLMNAQ
                     RIATMKPDAVLINTSRGGVVDEAALAGALREGRLGGAAIDVFEREPLPAGSPLAECPN
                     LLLTPHIAGVTRESNERVSSLIASKVAAALTSA"
     gene            complement(14257..14700)
                     /locus_tag="VFO53_04330"
     CDS             complement(14257..14700)
                     /locus_tag="VFO53_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8875536.1"
                     /translation="MANHLPTANYPRFHLAFPVTDLAQARRFYGALLGCAEGRSSDDW
                     VDFDFFGHQIVAHKVPAAAMTDATSLVDGRQVPVRHFGVVTDRATWDGLADRFRVAGV
                     RFVIEPYVRFAGEPGEQATMFLLDPFGNALEFKAFADMNQLFATS"
     gene            complement(14700..15569)
                     /locus_tag="VFO53_04335"
     CDS             complement(14700..15569)
                     /locus_tag="VFO53_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875537.1"
                     /translation="MAGEPPARPEILREPVSASAFGIVQKPLSTWERISNVGAVRKFA
                     LLVVIALVWEIYARELDNPLLFPTFSATVVAFFQGLFGGDLFTKALTSLRVLLMGYAA
                     GIVCAAVLTVVAITSRVGTDLLELLTSMFNPLPAIALLPLALIWFGLGAGSIVFVIIH
                     SVLWAVALNTHSGFLAVSNTLRMVGRNYGLRGLPYVARILIPAAFPSILTGLKIGWAF
                     AWRTLIAAELVFGVSSGSGGLGWFIYENKNQLEIPNVFAGLFTVIVIGLIVENVVFRV
                     IEAKTVRKWGLQS"
     gene            complement(15722..16525)
                     /locus_tag="VFO53_04340"
     CDS             complement(15722..16525)
                     /locus_tag="VFO53_04340"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875538.1"
                     /translation="MNTPAASAPLLSVRGVTLQYKTKDHLVTATYRVDFDVQRSDRFI
                     LLGPSGCGKSTLLKAVGGYMRPVEGEIRLKGDAVTRPGPDRMMVFQEFDQLLPWRTVK
                     ENVMFPLRSGGRLPRKAAEEKALEYIAKVNLTAFADSFPHTLSGGMKQRVAIARGMAM
                     EPAILLMDEPFAALDALTRRKMQDELLALWEGTRFTVLFVTHSIPEAIKIGSRILLLS
                     PHPGQVKAELDSLPRDASGAEVSALEQRIHAMLFADAIEDTTTAERVIA"
     gene            complement(16522..>17407)
                     /locus_tag="VFO53_04345"
     CDS             complement(16522..>17407)
                     /locus_tag="VFO53_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET8875539.1"
                     /translation="TPVMTCRAIDEEVGASLYFKCEHLQKVGAFKARGACNAVFSLDE
                     ASASRGVVTHSSGNHGAAIAYAAQARGIPAWVVMPRNAPAVKQANVRRFGANVRLCEP
                     TVAAREAACDEVARQSGATLVHPFDDARVIAGQATATLELLAAVDDLDVVIAPCGGGG
                     LLSGTAIAATSLAPGIRVFGAEPANADDAARSYASGAIEPLPSTTTIADGLRTTLSPR
                     TLLALCTHVSAFGICSEVAILRAMRMIFERMKQVVEPSSSVPLACLLERTLDVAGLRV
                     GIILTGGNVDLDQLEWTA"
BASE COUNT         2842 a         5857 c         5779 g         2929 t
ORIGIN      
        1 tcgagcttgg cgggagcctg cgcatcgagc ccgtgacgat cgcgctccgc tacaacgcga
       61 tcgatgcacg cttccgctcg acgttcgacg cggcaagccc cgacaattcc gaggcggatg
      121 aaaacggcgg catcgtcgtc cgcgccggca atcggatgcc cggcattccg gtgcagaccg
      181 cgaagctgcg cctcgactgg gacgtgaccc cgggcgttgc gatcggcgcg tcgctcgtcg
      241 cggcagcggg ccaatacgcc aacggcgacg agaacaacgc cgaccgcagc ggacgcgtcc
      301 ccgggtattt cgtgatgcat ctcgatgcgc agtacgtgat gacgccgcgc gtgacgctgt
      361 tcgcgaagat cgacaacctg ttcgaccggc gctacgcgaa tttcgggctg ctgggatcga
      421 acgtgttcac cggtccggaa cgcagcttcg gacccgccgt gggcatggcg ccggtgaacg
      481 agcagttccg cgcgctcggt tcaccgcgcg gcgttttcgt gggcgtccgg ctgaccctcg
      541 atgcgccggc gcatggcgtc cgcgattgat tcgacgctga tcgccgtcga gaacgcctgg
      601 tcctggttgt cgacgattct ggccgcgagc tggccgccgt tgcccggcgt gaaatagaag
      661 cggaaattgg ggttctcgct gatcgagaaa tcgacgtccg cgcgcatcac gggcttgccg
      721 gcgtacgtca cctccaccgt gcggacgaaa tgcggcgccg catacgtgcg ggccatcggg
      781 tccatcgcga gcccggaatc gttcgggtgg ctgatcatca gttgcgcgag gatcggccgg
      841 ccggggaccg gggtgccatc cactttcatc cgcattctgc cgagcgtcgc gagcgccgac
      901 gccggatcgc tgcccctcgg cgccgtgcaa ccgcccgacg ccttcacgta gctggtcgcc
      961 atgaagagcc tgccgtcgtt cgtccgcgcg attgcacgca catgcgtgta ctgctcgatc
     1021 cggatgcgcg tctcgacgtc ggcgcggccg ctttgcggcg tgaagtgaaa tatcgcggcg
     1081 accggcgacg ggttgttgtc gacgatcagc cagatcgtct cgatgaaatg cccggtcgac
     1141 tgcgtgaggc gcgtccggat cgcgacgggg acgatcgtcg cgtcgaccgc gcgcttcggg
     1201 gtgtcgagct cgacgacggc gtcgccgcgt tcgatcggtg catcggcgaa cacggtcgca
     1261 tgcagcttcc tccagacggc ggcgtcgtcg ggattttcgt cctgcgcgag cgcgaccggc
     1321 gcaagcccaa gcgccagtat cagcccgatc actgcgcctc gcatggggac tccttgcgtc
     1381 gggccgaccg cgccggcgcg agccgatcaa cctattcgct gcggatgaag cattgcttcc
     1441 tggcgttgtc ctgcagcttg cgatacgcgg cgatcagctt ccgcccatct ttgatgtcgc
     1501 gcatggcacc gccgcgctca cccgcgatgg tgatgccgtt ggcgatcgtc gaaagatcga
     1561 cccattcccg gtaaggaacc ttgtcggcga tagcgtcgat cgcgcacgag cacttgtaca
     1621 tcgcctcctg tggcgtcgcc ttgctctcct gcatgcacgt gagcacgaaa tcgacccggg
     1681 cgcgcgtggg gaagtcgttc gcgcttgcga gcgtcgcacc gcagatcgcg agcagcagca
     1741 ggtatcgcat gtcacgcagc cccgggacct acttgatcga ctcgccgcgc tcgtcgacga
     1801 gcgggacgtc gtactcgcgc aggatcgccg tgatggccgg cttgttctcg gcaatgagct
     1861 tgtcgacggt ggctctccat tcggcgtctc cctgccgcac gcccatcgcg atcgcatagt
     1921 cgaacttgac gcccggttcg gattgcagcg ggatcacgac cagctcgacg tcgtgcaccc
     1981 gcttggcgaa ataccccgcg atcggacccc agacgatggc cgcgtcgatc ctgccttgtg
     2041 ccaggtcctt gtcgatgatt tcaccgggat agcggtccgg atcgggcgac atcatcggaa
     2101 acggcttcgt ctgcgcctcc attccgtgct tgaccagcca atacgcggcg ggcgatcgat
     2161 cgtagatgcc gatcgtcagc ttgccccgga catcggcggg caacgcaagg aggtccgatc
     2221 cactctgcac ctgatcgagc ccgtgacctt tgcgatagac cagcgcgtac gtcgaccgat
     2281 agtacggtgc cgtcggccag gcctgatcgt acgacgccgg aacgctcatc acgatgtcgc
     2341 agcggaagtc ctcgccgggc aaccggtagc ggagcgtgtt gcgaatgaaa ttcatccgct
     2401 gcggaaacgc atagctttga accgggattc ccagcttctg cgcgaacaga tcggcgatgc
     2461 ggttctcgaa gccttcgcct ttctcgttcg cgaacgggag gttggaagga tcgatgcagg
     2521 gacgaaacgc ggtgcgtgcc gggtcggcga gagcgctggc gctcgccgtc gcgacgagca
     2581 gcgcggcgag caccgcggcc agccggcgga aagctgcggt cggcacggca cgcttctcgg
     2641 cgacgggcat ggcgcggcaa tgctcctcgg tcattgcttg agctcctcga cgtgcgcgcg
     2701 ggtgatgtcg ccgttggagc ggcctttcag atacgcgtac aggctgtcga tgttctccat
     2761 caccatcttg ctgtccttcc agctcaccat gcccttgtcg agccggccgt cgcgcaccgt
     2821 cttgatgaaa tcttccttgc tcatcgtctt caggctttcg atcagtgacg gtccgacgag
     2881 cccttcctgg ttggcgccat ggcagcggtc gcatgccgcc gcccgccatg cgcgaaaccc
     2941 gttcatcgtc gccgaatcga ccttgtagcc gtcgacgacc ttgtacagcg gcttgtcgga
     3001 cgacggcgcc ggagccttgt cctgcgcgct cacgggccct tgcgcagcga acagcagcag
     3061 cgccagcacg ccgatcatgt ttcgcatcga aactccatga cagtcactaa aagcgcgctg
     3121 cgtgatgcgg cgctgaatcg cgctgcgccg acgccgcggc caggcacgcg ttctgtggat
     3181 gagcatcggg attgccgcgc gagggttgca acgccgttcc agggaggaac gtcatcgccg
     3241 gcgcggcgtt cgagcgcacg ccggcgatga tcgaggccga ctacatcgga agcgcgaata
     3301 cggtcagcga gccacccagg ttggtgtagt cgcgaagctc cttgtagccg ccgacggcac
     3361 cgagcccctc ctgatccttc tcgaggcccg ccgccatgcc gattccggcc cagccgccga
     3421 tccccgagaa cacgcccatg tactgcttgc ctttgtgctg ataggtgaag acgttgccga
     3481 tgatccccga cggcgtcttg aagctgaaga gctccttgtt gatgttgttc gcatcgacgc
     3541 acttgagata gccctcgagc gtgccgtagc aaacgatccc tcccgcggtt gcgagcgggc
     3601 cgctccacac cgagaacttc tccgccttcg tctgcacgat cttgcccgtg ccggcgtccc
     3661 acgcgatgaa gttccccatg ttggtgccgc caggtgccgg gaacatcgaa agcgtcgccc
     3721 cgacgtacgg ctggcccgcc gtgtactcga ccttgaacgg ctcgtaatcc atgcagacgt
     3781 gattggtcgg cacgtagaac cgcttggtct tcggatcgaa tgccgccggt tgctcgtcct
     3841 tggagccgag cgccgccgga cagataccct tgttgttcac atcggtgccg ttttcgcccg
     3901 gtgcgtactt tttcacgacc tcgggccggc ccgtcttcat gtcgaccctg gttgcccagt
     3961 tcaccgccgg atcgtatttc tcggccacca gcagcgcgcc cgtcgcgcga tcgagcgtgt
     4021 aaccgaatcc gttgcggtcg aaatgcacca gcgccttcgt cggcttgccc ttcacgttga
     4081 tgtcggcgag gatcatctcg ttgatgccgt cgtagtccca ttggtcccac ggcgtcatct
     4141 gatagaccca cttcgcttca ccggtatcga cgtcgcgcgc ccagatcgcc atcgaccact
     4201 tgttgtcgcc gggacgctgc gccggattcc acgtcgacgg attccccgat ccgtaataga
     4261 gcaggttgag tcccttgtcg tagctgtacc agccccacgt ggtgccgccg ccgttcttcc
     4321 actgctcgcc ctgccacgtc ttcagcgacg aatccttgcc gacgggcttc atcgcaccgt
     4381 cggtccaggt catcgtcttt tccggattca cgagcatctc ttcatccgga cccatgctgt
     4441 acgcgcgcca cacctgcttg ccggagttga tgtcgtacgc ggtgatgtag ccgcgcacgc
     4501 cccactcgcc gccggagatg ccggtgatca ccttgtcctt gaatacgtgt ggcgcattgg
     4561 tgttcacggc gccgaccttg ggatcgccgt ttttcaccga ccagagcacc ttgccggtgt
     4621 tggcgtccag cgcgacgagc atgctgtcgg cctgctgcag gaagatcttg ttctccgcat
     4681 aggcgaggcc gcgatagacc gtgtcgcagc acatctgcgg aatgacggca gcgtcctgct
     4741 tcggctcgta acgccacttg atcttctgcg tatcgagatc gatcgcgaat accttgttgg
     4801 ggaacggcgt atgcaggtac atcgtgccgt tcatgacgag cggcgacccc tcgtgcccgc
     4861 gcagcacgcc ggtggaaaac atccacgcca cctgcatctt gccgacgttc ttcgcgttga
     4921 tctggctcag gttgctgtac cggtggttgt acatgtcccc ggcctgcatc gcccagttgt
     4981 ccggattggc gatcatcttc tcgagatcgg cgttcgccga tgcgctaccc ggaaacgcga
     5041 gcaaccatgc cattgccagc actgtctgga accacggctt gtcgacaatc ttcatagggc
     5101 cctccatggg tgatcgtcgt gtgtaccgcg ccggggaacg atgcgcggat cctgcttgac
     5161 cgggtagtgc gaatcgcgag ttggcgtgcg cagtgtctcg ccgcgttcgg gcaaagtcaa
     5221 tttgagcagc acaattcgtg tcgacctggg aaagacatgc gatgctgcgc tgcagcaagc
     5281 tgtcgcattc cctggggcgt gataatcgca ctcgtgatgt catccgctgc cggcgcgctg
     5341 gcgcgattcg catgccttct gctggcgctg gcgtgcgcat cggcgcatgc gtcggcgccg
     5401 ctggccgtgg cgcccggcgt gttcgtcctg atgggacggg acggcgagat ttcgccggat
     5461 aacctcggcc gcatcgcgaa tatcgcgttc atcgtcggtc cgcgcggcgt tgtcgtcgtc
     5521 gacagcggcg tgtcgtttcg tcagggtgaa gcgatcatcg ccgccgttcg gcgcgtgaca
     5581 cgccggccga tcaggctcgt cgtcatgacg cacgcgagcc gggaagtcgt gttcggcgcc
     5641 gcggcgttcc aggcgcatgg gatcccggtg tggatgcacg ccgccgctgc cgccctgatg
     5701 gcgtcgcgct gcgacgcatg cctgaccggg cttgccgaaa cgctcggcgc cgaggccatg
     5761 gcgggcagcc gcgtcgtcgc gccggatcgc ctcgtggcgg gcaccctgtt gctcgacgtg
     5821 atcggccggc cgctgatggt cgttgcgcca gcgcagggga gcgcgcccgg cgcaatcgcg
     5881 gtattcgacg tcgccacgcg aacgttgatc gccggcacgc tggtttcgat cgaccgcgta
     5941 cccgacatgc acgacgcttc cggcccctgg cgcggcgcgt tggcgatgct tgcggcgacg
     6001 cattgcgtgc atctcattcc cgcttacgga cgcgttggca gctgcgccga tatcgacggc
     6061 atcgagcgct atttcgcggc gctcgagcag cgtgtgcgcg agctgctcgc ggcggacgtc
     6121 ggccttgccg aattgccggc ccgatgcgac ctccccgaat tctcggcctg gggccggtac
     6181 ggcgcgctgc acgtcgggaa cgcgagccgg gaatacctca ggatggagcg cgcatcgttt
     6241 aaagatcgag actggaaacc caccggagta aaggactaag atcgcggtct cgccggacgc
     6301 gagaggagga tcgaccatgg atgtgcgcgc agcggtggct tacaaggccg ggcagcccct
     6361 ggtcatcgaa tcggtgcagc tcgccggccc gaaagcgggc gaggtgctgg tcgagatcaa
     6421 ggcgaccggt atctgccata ccgacgaatt cacgcgcagc ggcgcggacc cggagggcct
     6481 gtttccggcg atcctcggcc acgagggcgc gggtatcgtc gtcgatgtgg gacccggtgt
     6541 gagcagcgtc gccaaaggcg accacgtcat cccgctctat acgccggagt gccggcagtg
     6601 caagtcgtgc ctgtcgcgca agaccaacct gtgcacggcg atccgcgcga cgcaaggcag
     6661 gggcgtgatg cccgacggca cgagccggtt ctcgcttcgc ggcgagccga tccatcacta
     6721 catggggtgc tcgacgttcg cgaacttcac cgtccttccg gaaatcgccg tcgcgaagat
     6781 ccgccaggac gcgccgttcg agaaggtctg ctacatcggc tgcggtgtca cgacgggcat
     6841 cggtgcggtg atcaacacgg cgaaggtgga agccggcgcc aacgtcgtcg tgttcggcct
     6901 cggcggcatc ggcctcaacg tcgtgcaagg ggcgcgcatg gtcggcgcga acatgatcat
     6961 cggcgtcgat ttgaatcctg cgcgcgaggc gctcgcccgc aagttcggca tgacgcattt
     7021 cgtcaatccg aaggacgtca agggcgacct cgtggcgcac ctcgtcgagt tgaccggcgg
     7081 cggcgccgat tattcgttcg aatgcatcgg caacgtcgac gtgatgcgcc aggcgctcga
     7141 gtgctgccac cgcggctggg gtgtcgccgt gatcatcggc gttgccggct cgggccagga
     7201 gatccgcacg cgtccgttcc agctcgtgac cgggcgcgta tggaaaggga cggcgttcgg
     7261 cggcgcgcgc ggccgcaccg acgttccgaa gatcgtcgat tggtacatgg acggaaagat
     7321 caacatcgac gatctcatca cccacgtgct gccgctcgat cgcatcaacg acggcttcga
     7381 cctcatgcac gcgggcaagt cgattcgcac ggtggtcacc tattgaacgg aaactcttga
     7441 cgaggagctg acagaccatg cctacaccat ccattcatcc cgccgtcgat cacggcatca
     7501 agccggcggc cgcgaacttc tccggcggaa cgctgtattg caactgttcg gaaggaaagg
     7561 tcgccgtctc gatcaagggg caaacggcgc acaaccacgt atgcggctgc acgaaatgct
     7621 ggaaaccgag cggcgcgctg ttctcgcagg tcgccgtcgt gtcgcgcgac aaggtcagcg
     7681 tcagcgccaa cgggaacaag ctgcaggtcg tcgacccgag tgccaccatc cagcgccatg
     7741 catgcaagag ctgcggcgtg cacatgtacg ggcgcatcga gaacacgggc catccgctgt
     7801 acggcctcga tttcgtgcat accgagctgt cgcccgagca gggctggtcg ccgcccgagt
     7861 tcgccgcgtt cgtctcgtcg atcatcgagt cgggcgcgaa tcccgcgaac atgggcgcgg
     7921 tgcgcagccg cttgaaggaa atcgggctcg agccttacga ctgcctctcg ccgccgatca
     7981 tggatctcat cgcgacgcat acggcgaagg ccaagggcgt gctcgccagc gcgtagccac
     8041 gctccggcag cgacgcatcg cacgcgaggc tcggccgcgc cggccggggc ctcgcggccg
     8101 aatgccgtcg ccgaagaaaa tcgtgtcgca agcgccccgg cccctttccc agaatcgcag
     8161 cttcggcggc gtgcagtacg tctatgcgca cgccgccgag accacgcgtt gcgacatgcg
     8221 cttcggcgta ttcctcccgc cgcaggcaac gtctcgcgcc gtgccggtcc tctactggct
     8281 gtcgggcctc acgtgcaccg aggaaaactt catcgtcaag gccggcgcgc agcgcattgc
     8341 gtcggagctc ggattggcga ttgtcgttcc cgacacgagc ccgcgcggcc tcggcattcc
     8401 tggcgaggcg gagagctacg acttcgggct cggcgccggt ttctacgtcg atgccaccga
     8461 ggcgccgtgg tcgcgcgggt accgcatgta ttcctacgtg acgaaggaac tgccggcgct
     8521 cgtcgaagaa catctgcccg tcgacgccga gcgcgtcggc atcttcggcc attcgatggg
     8581 aggtcatggc gcattgacca tcgcgctgcg cgatcccgat cgttaccgaa cggtttcggc
     8641 gtttgcgccg atcgcctcgc cgatgcattc cccgtggggc gagaaggcat tcaccggcta
     8701 tcttggcgcc gatcgcgatc gctggcgcga ctacgacgcg accgcgctgc tcgatgcacg
     8761 cggctggccg ggaccgccgc ttttcgtcga tcagggaacg gacgacccgt tcctcgagcg
     8821 cgagctgaag cccgggctgc tgaagggggc gtgcgagcgt gccggcgtcg agctcgagct
     8881 gcgcatgcag cgcggctacg accacagcta ccatttcgtc gcgacgttca tcgaggatca
     8941 tctgcgcttt cacgcgagcc ggctttcgag cgcgcctgcg ggcggatcgt gaagcggcgc
     9001 atgcgacggg cgatggtcgc ggcattcgtc gcgctggccg gcgcgtcgtc gccggatgcg
     9061 gccgacttca cggcggatca ggtgcgccag cggcttgcga acgcaagcga cggcaagtcc
     9121 gccgacttca cgggcaagga tctgtccgat ctcgacctcg gtcacatcga ctttcgacgt
     9181 gccgatctgt ccggcgccaa cctgttcgcc tcgcgactcg tttcatccaa cctcgcgggc
     9241 gccaagctcg caggcgccaa cctgaacggc gcgtggctca tgggcgcttc gttcgccggt
     9301 gcggaccttt ccggcagcac gatgctgggg ctcgtgattc tcggcggcga cgtcaaggag
     9361 aagccgaatt tcgccggggc caacctgtcc ggcgtgcgca tgatcgccga ccttccgggc
     9421 gccgatcttc acggcgcgaa cctgcgccgc gcgcggctcg gcgtcgacat gcgcaaccag
     9481 ggcatggggc agatgcgcac cgacttgagc aacgcgaacc tgtcgggggc cgatttgcgc
     9541 gacgcggacc tcagccggtc ggtgatgatg ttcgccgacc tccgcggcgc cgacttgcgc
     9601 ggcgcgaacc tgttcggtgt caagcttgca ggagcaaacc tgaccggcgc gcagatcgct
     9661 gacgccgact tcaccgaagc ggatctcgac gcaaccgttt tcacgggcgt gcgggaccgc
     9721 gatgctgcga agggcctgga caaggccatc aacggcgagc gcgccatccg ctgaatcggc
     9781 ccgcccgccg cgtacggctc cgcgcgtcgc cggcggccaa tccggtcacg cgattttggc
     9841 cgtcacgcgc tttcgctaca cgcgtatgcc gggattcggg cgctggccac cttcctgcgg
     9901 cgactgctcg tccggcggcg tggtgggcgc gttctcatcc tgcgtcgttc ccggcggcgt
     9961 ttcctcctgc gtgcttcctt ggggtgcttc atcctgcgcc gttcccggcg gcgtttcctc
    10021 ctgcgtgctt cccggggacg cttcgtcctc ggccgctccc ggtgacttct ggtcggggac
    10081 cgcccggtcc atcaggctgg tcggcggatg cttggccgaa tcgggctcgc ccgtcgtcgt
    10141 tttgccggat ggcgtcgccg catccatctg cgcggtcggc gggtgcttca cgccggtgtc
    10201 cggccgctgc gccggctggc cctgcgcgct gccgaggggc gggaatgtca atcccattgc
    10261 aagggcggcg gctgccgtca gacgtgaata gttcatcgcg tgcctccgga ttcgcttgtc
    10321 tcgtaagctc acgatgcacc ggcgccgccc gccgcgccat ccgcgattga tgggattcgt
    10381 cgtaggcgcc caccggtgtt cgtgtcagac ggtgaccggc atggcgtcca tgagcggcat
    10441 caaccccagg gtgaatgcct ccgaaatctg gaataatcga tcgtcgccgc ggtacgcgtc
    10501 gcggcggaac gcgacgcggc ggcgtcgccg aaccggcgcg atcctcccat ccgaaaactg
    10561 gagtacgacc atggccgcag ccaagctgat cgtgatctat ccgacgcccg cgaatctcga
    10621 agccttcgag cacgcgtaca gcaccgggca catcccgatg gccgcaccga tcttcgaggc
    10681 cgccggcgcg acgaaggtcg tcctgacgct cgtttccgct gcggcaagcg gcccggcgcc
    10741 gtatcaccgg atcgccgaaa ttcacttccc ttcgatgcag gcgttgaacg cgtgcgcggc
    10801 atcgcagggt ggacgcgatg cgctggcgga cgccgctcgc atctcgaacg ggggcgcgcc
    10861 gatcgtgctg atcgccaccg aggacgtggt gacgttcgcg ggccgctgac gggcgtcagt
    10921 cgttcgcggg cggctgacgg gcgtcagtcg cccggctgcg gcgcggtatc gtcgacgccg
    10981 agcccgccca tcgcggtgcg cggcagcagc atcaccacgc agaacgtggc gaatgcaatg
    11041 gccgccatca acagcaggag cgcgctgaac cccctcgcct tgacgatcgg gccgaggagc
    11101 cacaccgcaa gcgagctcac gccgaatgcg accgccaggc gcatgcccga cacccgggag
    11161 cgcatccggt cgtcgacgta gcgcacgatc atcgcgtcgg taaacggaat cgcaccgaac
    11221 acgacgatca tgaatgcgaa catcagcgcg taaagcaccc acccggtggc attcgcggcc
    11281 agcaggaaga gcggcgcctg cacgacgacg atgcacgcgt aaagcgtctt gagcggcacg
    11341 cgatcgagca gcaatccgac actgacctgc gcgagggcac cggcaacata gaccgccgcg
    11401 agcagcatcc cgagcgccgc cggatcctcg acgatgccgc gcagccgttc gcgcaacaac
    11461 tcgccgttgc cgttggtggt gaagttgaac agcaggctgc cggaaatcga cgcgacggtg
    11521 acgacggcaa agatgtgcgc gagcgcacgc ttgccgagca tcgcgcgcga cggcgcgcgt
    11581 cgcgccggcg ccaccccttc ggcgggcgcg acgcgggcga acagcacgcc gcagccgatg
    11641 gcggcaaccg cgggcacgac gaacgccgcc tgccagccgg cgtatttgag gagcgcgccg
    11701 gtgacgagcg ccgacatggc gacgccgaga tttccggcaa ggccgttgaa gccgatcgtg
    11761 agtccaggcc gcgcggcgcg ctgcacgagc atcggaatgc ccaccgggtg gtagatcgcg
    11821 gagaaggcgc caagcagcgc cagcgccgcc gcgagctgcc atgccgagtg cgtgaatgcc
    11881 acgagcagcg cgcaggcgcc catgccgaag aagaacacga gcatcatgcg ccgtcttccc
    11941 cacagatcgc cgaggcgccc cgcgggaagc gagccgatcc cgaacatcgc gaaggcgccg
    12001 acgccgtacg gcatcaggtc ttcccagtgc gcgaagccga agtcggcggc aatggcggtc
    12061 accgaggtgg cgaagatcag caggaacatg tgatccatcg catgcgcgac attgagtagc
    12121 agcgaggtcg cgcgcggcag cggcgtcatg acgatagtct cccggccacg cgatcggcat
    12181 tccagcgcga cgtgctcgcc cacgcgtgga attcgcctac gggcagcggc ctgccgacat
    12241 ggtaaccctg cgcggcgtcg cggccctcgt cattgcgcaa gcgcgcgcaa ctgctcggcg
    12301 agcgcctggg aaatttccac gccgtgcacg gacgcgctgg ccgcgagtgc cgcgcgccgt
    12361 gcaccgggaa ggcgcacccc ctcgtcctgc tgcatcgccg cgatcagcgt ctcgatgcgc
    12421 tcgtcgaaca cgtcgcgtcc ggcaagcgca tccggatcga tcacgaggaa cgcctggccg
    12481 atgcgcggcc gattgccttc gtcgacgaag aacgagctcg cctcgaagcc gagtgcggca
    12541 cccgtcaatg cggtgacgag caactcgacg accagggcca gcatcgcgcc cttcgttccg
    12601 cccatcgcga gcatcgagcc tgcgagcgcg gccttcgcat cggtggtcgg ttgtccgtcg
    12661 cgatcgagcg cccagccgag cggaatgggc ctgccctcct tcgccgcgac catcaccttg
    12721 ccgcgcgccg cctcggacag cgacaggtcg atcgacagcg gcggcgcgtc gcgccgcgga
    12781 aagacggcgg cgatcgggtt ggtgccgaag agcgcgcgcc tgccgcccgc cgcaggcatc
    12841 gccgccggcg aattggcgaa cgcgagcgcg accatgccgg cggcggccgc cggctcgaga
    12901 tgccacgcgg cgacgccgaa atggtggctg ttcgtgacac ccgcgaacgc aatgccgcag
    12961 tcgcgcgcac gccgtatcgc ctcgtcgacc gcaagcgtgc acgcgggaaa tgcaaggccg
    13021 catcgcgcat cgacgagaat ggcgccgccg cgctcgcgcg caatgacggc gaccgcgcga
    13081 ccgtcggcgc ggccgttgcg cagatgcgtc gcgtattgcg aaacgcgcgc catgccgtgc
    13141 gacgacagtc cctgcgcctc ggcatcggcg agcgcgcgcg ccgtgatgct cgccatcgcc
    13201 gcggacgcgc ccgccttggc gagcgcattc tgcgcgagat cgcgcagctc ggatacggtc
    13261 agcgtcggca tcgggcgcgc ccttgttgac ttacgcggag gtgagcgccg cggcaacctt
    13321 cgatgcaatg agcgacgaca cacgctcgtt ggattcgcgc gtcaccccgg cgatgtgcgg
    13381 cgtgagcagc aggttcgggc actcggcgag cggggagccg gcgggcagcg gctcgcgctc
    13441 gaagacgtcg atcgccgcgc cgccgaggcg gccttcgcgc agcgcgccag caagcgcggc
    13501 ttcgtcgacg acgccgccgc gcgacgtgtt gatcagcacg gcatcgggtt tcatggtcgc
    13561 gatacgttgc gcgttcatca ggttgcgcgt cgccgccgcg agcggcacgt gcagcgaaac
    13621 gacgtcggct tctccgagca gttcggcgag cgtgcacggc cgcgtcgcgt cgtccgccca
    13681 tacgggcgcc gccgcaggaa tctgcggatc gaacgcgatt gtgtgcatcc cgagtgcgcg
    13741 cgcgagacgg cccgtcagcc ggccgatgcc gccgaagcca atgagcccga gcgtcctgcc
    13801 cccgagttcg cggccgccgg atagccgtgc gcgtggccag cgccccgccg taacgtcggt
    13861 ggttgcgaaa tacgcgccgc gcagcaacag catggcggcc gcgatcacgt actcggcaac
    13921 cgcctgggca ttggcacccg tggccgggat caccgcaatg ccacgcgtcg cgcaggcgtc
    13981 catgtcgagg ttgtccagcc cgacaccgag acgcccgacg acgcgcagct tcggcgcggc
    14041 cgatacgagc gcggcgtcga cctgggtgcg gttacgcacg atcagcgcgt cggcatcgtg
    14101 caggtcggcg acgagctcgc tgcgccggtc gaccagatcc ttgtcgtagc ggacgtcgaa
    14161 acgcgacgca agctccgcga ccgccgggct gtccatgaat tcgctgatga cgatgcgcac
    14221 gagcgtcgac agggggtctg acgagcgaac gccgcgtcag gatgtcgcga aaagctggtt
    14281 catgtcggcg aatgccttga actcgagcgc attgccgaag ggatcgagca ggaacatcgt
    14341 cgcctgctcg ccgggctcgc ccgcgaagcg gacgtagggc tcgatcacga agcgcacgcc
    14401 ggcgacgcgg aagcggtcgg cgaggccatc ccatgtcgcg cgatcggtga cgacgccgaa
    14461 atgcctgacc ggcacctgcc tgccgtcgac gaggctcgtt gcgtcggtca tcgccgccgc
    14521 gggcaccttg tgcgcgacga tctggtggcc gaagaaatcg aagtccaccc agtcgtcgct
    14581 gctgcggccc tcggcgcagc cgagcagcgc accatagaac cgccgcgcct gcgcgaggtc
    14641 ggtgaccgga aaggcgaggt ggaagcgcgg gtagttcgcg gtcggaagat ggttggccat
    14701 cagctctgca gtccccactt gcgcacggtc ttcgcctcga tgacgcggaa cacgacgttc
    14761 tcgacgatga gaccgatgac gatcacggtg aaaaggccgg cgaagacgtt gggaatctcg
    14821 agctggttct tgttctcgta gatgaaccag ccgaggccgc ccgaccccga cgacacgccg
    14881 aacacgagct cggcggcgat cagcgtgcgc catgcgaacg cccagccgat cttgagcccc
    14941 gtcaggatgc tcgggaaagc ggcggggatc aggatgcggg cgacgtaggg caatccgcgc
    15001 aggccgtaat tgcgcccgac catgcgcagc gtgttcgaca cggcgagaaa gcccgaatgc
    15061 gtgttgagcg cgaccgccca cagcaccgaa tggatgatca cgaacacgat gctgccggcg
    15121 ccgaggccga accagatcag cgcgagcggc aaaagcgcga tcgctggcag cggattgaac
    15181 atcgaggtca aaagctcgag cagatcggtg ccgacgcgcg acgtgatcgc gacgacggtc
    15241 aggacggcgg cgcacacgat cccggccgcg tagcccatca gcaggacgcg cagcgacgtc
    15301 aatgccttcg tgaagaggtc gccgccgaac agtccctgga agaacgccac gaccgttgcc
    15361 gagaacgtcg gaaacagcag cggattgtcg agctcgcgcg catagatctc ccagacgagc
    15421 gcgatcacga cgagcagcgc gaacttgcgc accgcgccga cattgctgat gcgctcccat
    15481 gtcgataacg gcttctggac gatgccgaat gcgctggccg agaccggttc gcgcaggatt
    15541 tccggccgcg cgggcggctc gcctgcgatc gcgcttgtat gatcccccca cgcttgcggc
    15601 cgattgaacg tccctcgtgc ttcgcactcg gctgtttgcc ctcggggcgg cccatcgggc
    15661 tcacgcgctg cgctgccccc ggcgggggtg caattcgatc cttggggcgg ccctgcggat
    15721 ctcatgcgat gaccctttcg gcagtggtcg tgtcctcgat cgcatcggcg aacagcatcg
    15781 cgtggatgcg ctgctcgagc gccgagacct cggcgccgct cgcgtcgcgc ggcaggctgt
    15841 cgagctcggc tttcacctgc cccgggtgcg gtgacagcag caggatgcgg ctgccgatct
    15901 tgatcgcctc gggaatcgaa tgcgtgacga agagcacggt gaagcgggtg ccttcccata
    15961 acgcgagcag ctcgtcctgc atcttgcgcc gcgtcagcgc gtcgagtgcc gcgaatggct
    16021 cgtccatcag caggatcgcc ggctccatcg ccatgccgcg cgcgatcgcg acgcgctgct
    16081 tcatgccgcc ggagagcgtg tgcgggaagc tgtcggcgaa cgccgtaaga ttcaccttgg
    16141 cgatgtactc gagcgccttc tcctccgcgg ccttgcgcgg caggcgcccg cccgagcgca
    16201 gcgggaacat cacgttttcc ttgacggtgc gccacggcag caactggtcg aattcctgga
    16261 acaccatcat ccggtccgga ccgggacgcg tcaccgcgtc gcccttgaga cggatctccc
    16321 cctcgaccgg cctcatgtag ccgccgaccg ccttcaggag cgtcgacttc ccgcaaccgg
    16381 agggtccgag caggatgaag cgatcggagc gctggacgtc gaagtcgacg cgatacgtgg
    16441 ccgtgacgag atgatccttc gtcttgtact gcagcgtcac gccgcgcacc gacaggagcg
    16501 gtgccgaggc tgcgggcgtg ttcatgcggt ccactcgagc tggtcgagat cgacgttgcc
    16561 accggtgagg atgataccga cgcgcaagcc ggcgacgtcg agcgtgcgct cgaggaggca
    16621 cgcgagtggc accgacgacg acggctcgac gacctgcttc atccgctcga agatcatccg
    16681 catcgcgcgg aggatagcaa cttcgctgca gatgccaaag gccgagacat gcgtgcacag
    16741 agcgagcagc gtgcgcggcg acagcgtggt gcgcaatccg tcggcgatcg tcgtcgtcga
    16801 gggcagcggc tcgatcgcgc cgctcgcgta gcttcttgcc gcatcgtcgg cgttggccgg
    16861 ctcggcgccg aacacgcgga ttcccggtgc gagggacgtg gccgcgatcg ccgtgccgga
    16921 caacagaccg ccgccgccgc atggcgcgat cacgacgtcg agatcgtcca ccgcagcaag
    16981 cagctcgagc gtcgccgtcg cctggcccgc gatcacgcgc gcatcgtcga acgggtggac
    17041 cagcgtggca ccgctttggc gagcgacctc gtcgcacgcc gcctcgcgcg cggccaccgt
    17101 gggctcgcac aaccggacgt tcgcgccgaa ccggcgcacg ttcgcctgct tgacggccgg
    17161 tgcgttgcgc ggcatcacga cccacgccgg aatgccgcgc gcctgggcag cgtacgcgat
    17221 cgccgcgccg tggttgcccg acgaatgcgt gacgacgccg cgcgaagcgc tcgcctcgtc
    17281 gagcgagaac acggcattgc atgcgccgcg cgccttgaat gcgccgacct tctgcagatg
    17341 ctcgcacttg aagtaaagcg acgcgccgac ctcctcgtcg atcgcgcggc atgtcatcac
    17401 cggcgtg
//
LOCUS       DASSVX010000094         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1252894, whole genome shotgun sequence.
ACCESSION   DASSVX010000094 DASSVX010000000
VERSION     DASSVX010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252894"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..522
                     /locus_tag="VFO53_04350"
     CDS             127..522
                     /locus_tag="VFO53_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HET8875540.1"
                     /translation="MPIYAYRCTSCGHELEALQKLSDARLTECPACHAAALIKKVSAA
                     GFQLKGSGWYATDFRNSGAKQPAKDAAAKTGDANAGDKANGAATKTDAKSGDGATTES
                     TTTTTKTSGVAGATESKSTTTSTADKTGT"
     gene            537..1205
                     /locus_tag="VFO53_04355"
     CDS             537..1205
                     /locus_tag="VFO53_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HET8875541.1"
                     /translation="MRRYLIAGLLVWVPLGITLWVLHFLLTSLDQVLLVLPESAQPRA
                     LFGFDIPGLGVVVAFLILFATGVIAANFFGQRLIRAWEAVLGRIPFVKSIYSSVKQVS
                     DTLLSDKGQAFRKALLVEFPRPGSWTIAFQTGVPAVSVAPLLPGEHISVYVPTTPNPT
                     GGYFLMLPRAQVRELDMTVDEALKYIISMGVVAPRPNARAVRGAGTDAAAHRAPSTSS
                     EIRN"
     gene            1213..>2793
                     /gene="aspS"
                     /locus_tag="VFO53_04360"
     CDS             1213..>2793
                     /gene="aspS"
                     /locus_tag="VFO53_04360"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET8875542.1"
                     /translation="MKRTDYCGRIDRRYLGNDVTVMGWVHRRRDHGGVIFIDLRDREG
                     IVQVVCDPDRAETFGIAEKLRSEYCVAVTGRVRPRPEGTVNANLPSGEVEILAQSIDI
                     LNASATPPFQLDDEHLSETVRLSHRVLDLRRPQMQHNLMLRYRAAMAARRYLDAHGFV
                     DIETPVLYKSTPEGAREFLVPSRMHHGQFYALPQSPQLFKQMLMIAGFDRYYQIVKCF
                     RDEDMRADRQPEFTQIDIETAFLDELEIRAMMEGLVRAMFRDAMALELPDPFPVMTYD
                     DAMRDYGSDKPDLRVPLKLTELTDVMRSVDFKVFRAAAELRHGRVAALRVPGGGSMTR
                     KEIDDYAVLAAIYGAKGLAYVKVNDASKPSEEGLQSPIVKFLASDVLRTILERTGAQS
                     GDVIFFCADRAKVVNDTLGALRARIGHERGLFEAGWKPLWIVDFPMFEQDDATGAWNA
                     RHHPFTAPKDAHAESFATDPEHAIAKAYDVVLNGWEIGGGSVRIHRPDMQARVFEALG
                     IGVDDQRRKFGFLLDALQY"
BASE COUNT          489 a          946 c          901 g          457 t
ORIGIN      
        1 cggtgccggg cgcaagcata agacatgcgc gcatatcgcg aaaggccggt ttccggtata
       61 atttcccgtt tgttatcaaa agcttttgcg cgcgttttcg caaccccccg cgcgcggtgg
      121 acaatcatgc cgatctacgc ataccgctgc acgtcctgcg gccatgagct cgaggcgctg
      181 caaaaactct ccgatgcacg cttgaccgaa tgcccggcct gccacgcggc ggcgctcatc
      241 aagaaagtgt ccgccgccgg ttttcagctc aagggctccg gctggtacgc caccgacttt
      301 cgcaacagcg gtgcgaagca gccggccaag gatgccgccg ccaagacggg cgacgccaac
      361 gcgggtgaca aggccaacgg cgccgcgacg aaaaccgacg cgaaatcggg cgacggagcc
      421 acgaccgagt cgacgacgac cacgacgaag acgagcgggg tcgccggcgc gaccgaatcg
      481 aagtcgacca ccaccagcac ggccgacaag accggcacct gatttacacc gcgacgatga
      541 ggcgctatct gatcgcgggc cttctggtct gggtgccgct cggcatcacg ctctgggtcc
      601 tgcacttttt gctgacgtcg ctcgaccagg tcctgctcgt gctgcccgaa agcgcgcagc
      661 cgcgtgcgct gttcggtttc gacattccgg ggctcggcgt cgtcgtcgcg ttcctgatcc
      721 tgtttgcaac cggcgtcatc gccgccaatt tcttcggcca gcggctgatc cgtgcgtggg
      781 aagcggtgct cggacgcatc ccgttcgtga agtcgatcta ttcgtcggtg aagcaggtca
      841 gcgacacgct gctgtccgac aaggggcagg cgtttcgcaa ggcgctgctc gtcgagtttc
      901 cgcgacccgg aagctggacg atcgcgttcc agaccggtgt cccggcggtc agcgtcgcgc
      961 ccttgctccc gggcgagcac atcagcgtct acgtgccgac aacgcccaac ccgaccggcg
     1021 gctatttcct gatgctgccg cgcgcgcagg tgcgcgagct cgacatgacc gtcgacgagg
     1081 cgctcaagta catcatttcg atgggcgtgg tcgcgccgcg accgaacgcg cgcgccgtgc
     1141 gtggcgcagg gaccgatgcc gccgcgcatc gtgcgccgag cacgtcctcg gaaatccgga
     1201 actgagaacg atttgaaacg caccgactac tgcggccgca tcgaccggcg ctacctgggc
     1261 aacgacgtga ccgtgatggg atgggtgcac cggcgccgcg accacggcgg cgtcatcttc
     1321 atcgacctcc gcgatcgcga aggcatcgtg caggtcgtgt gcgatcccga ccgcgccgaa
     1381 accttcggca tcgccgagaa gctgcgcagc gaatactgcg tcgcggtgac cgggcgcgtg
     1441 cgcccgcggc ccgaaggcac cgtcaatgcg aatctgccgt ccggcgaagt ggagatcctg
     1501 gcgcagtcga tcgacatcct gaacgcatcg gccacgcctc cgttccagct cgacgacgag
     1561 cacctgtccg agaccgtgcg gctttcgcat cgcgtgctcg acctgcgccg gccgcagatg
     1621 cagcacaacc tgatgctgcg ctatcgggcg gccatggcgg cgcggcgcta tctcgacgcg
     1681 cacggcttcg tcgacatcga gacgccggtg ctctacaagt cgacgccgga gggcgcgcgc
     1741 gaatttctcg tcccgtcgcg gatgcatcac gggcagttct acgcgctgcc gcagtcgccg
     1801 cagctgttca agcagatgct gatgatcgcc ggcttcgacc gctactacca gatcgtcaag
     1861 tgcttccgcg acgaggacat gcgcgccgac cgccagcccg aattcacgca gatcgacatc
     1921 gagacggcgt ttctcgacga gctcgagatc cgcgccatga tggaaggact cgtccgcgcg
     1981 atgttccgcg acgcgatggc gctcgagctg cccgatccgt tcccggtcat gacgtacgac
     2041 gacgcgatgc gcgactacgg ctcggacaag cccgacctgc gcgtgccgct gaagctcacc
     2101 gagctgaccg acgtcatgcg cagcgtcgac ttcaaggtgt tccgcgccgc ggccgagctg
     2161 cgccacgggc gcgtcgccgc attgcgcgtg cctggcggcg gctcgatgac acgcaaggaa
     2221 atcgacgact acgcggtgct cgccgcgatt tacggcgcga aggggctcgc ctacgtcaag
     2281 gtgaatgacg cctcgaagcc cagcgaggaa gggctgcagt cgccgatcgt caagttcctg
     2341 gcgtccgacg ttctgcgcac gatcctcgag cgcaccggcg cacagtcggg cgacgtcatt
     2401 ttcttctgcg ccgatcgcgc gaaggtggtc aacgacacgc ttggcgcatt gcgcgcgcgc
     2461 atcgggcacg agcgcggcct cttcgaagcc gggtggaagc cgctgtggat cgtcgacttt
     2521 ccgatgttcg agcaggacga cgccacgggt gcgtggaacg cccgccatca cccgttcacc
     2581 gcgccgaagg acgcgcacgc cgagagcttc gccaccgatc ccgagcacgc catcgcgaag
     2641 gcctacgacg tcgtgctgaa cggatgggag atcggcgggg gatcggtgcg catacatcga
     2701 cccgacatgc aggcgcgcgt gttcgaggcg ctcggcatcg gcgtcgacga ccagcggcgc
     2761 aagttcggct tcctgctcga cgccttgcaa tac
//
LOCUS       DASSVX010000095         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_436391, whole genome shotgun sequence.
ACCESSION   DASSVX010000095 DASSVX010000000
VERSION     DASSVX010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436391"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1014)
                     /locus_tag="VFO53_04365"
     CDS             complement(25..1014)
                     /locus_tag="VFO53_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8875543.1"
                     /translation="MTNAATADSTSPLDFVGVDMAQQAFDWALHGAPGTHRASNDDSG
                     FHALLGALQGRRLGLIVLEATGGFERALATFLLGHGMPVAVVNPRAAREFARSMGHLA
                     KTDAIDAIVLAHLAKTLAAKADQAGIAFVPPSAAVGALQTRVARRGQLLAMRLAERNR
                     LGGPMRVLQRSIETVIKTLDKEIAKLDRDIDAHLQRHFNEQLQRLNAIKGVGTLTCAT
                     LLAFLPELGTVNGARLAKLVGLAPLNNDSGKERGKRHIWGGRPVVRCALHMAMLSAIR
                     YNPVIKTFYKRLLAAGKPKKVALTACSHKMLRILNAMARTGLPWNEHLHAITP"
     gene            1230..3452
                     /locus_tag="VFO53_04370"
     CDS             1230..3452
                     /locus_tag="VFO53_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET8875544.1"
                     /translation="MSAPVTHAGRRAFLARSGALVVTFSLAPLQVAAQEKALAAKLPG
                     SLDKTPLLDAWIRVGADGSVTVFTGKAELGQGVKTALIALAAEELGIAPPAIVLVTAD
                     TSRTPDEGYTAGSNSMKDSGTAIRSAAADVRELLIERASAKLGVPPERLHAQNGNVIA
                     DDGRRIGFGDLAAQGYAEVRAKPNAKLKDPRSYTIVGRRLARVDIPAKVTGGIAYVQD
                     LRPPAMLHARVVCPPSHRARLREADVAAVARMPGVVKVVRDGGVLGVVAAHEFEAIRA
                     MRALAATARWDEEPSLPRGDLFAALQALPSQEIVNADRHAGASQGMRTLEATYTRAYQ
                     MHGSIGPSCALALYQDDAMTVWTHSQGVYPLRASIAEMLQMQAERVHCIHAEGSGCYG
                     HNAADDAAAYAALFARAVPAHPIRVQWMREQEHACEPYGPAMIAKLSGSVDASGSIAE
                     WNVEGWSNSHSTRPGGAGNLMPAWYLATPFAQPVPKPIPMPEGGGDRNSIPIYTVPSL
                     RVVHHFIPDMPVRVSALRSLGAYVNVFAIESFIDELAHAAGADPVAYRIRHLGDRRAR
                     DVVSAAATRFGWEGFTRKPGHGRGFAFARYKNLAAYCAVAVELTVDHETGRARVVRAT
                     AAIDSGQAVDPDGLANQIEGGIIQSLSWTLFEAVKYDATRITSVDWSTYPIARFADIP
                     DRIDVHVIDQPGEPFLGTGEAAQGPTAAAIANAIADARNVRLRDLPLTAEKIKVAVGV
                     "
     gene            3478..3768
                     /gene="dapD"
                     /locus_tag="VFO53_04375"
                     /pseudo
     CDS             3478..3768
                     /gene="dapD"
                     /locus_tag="VFO53_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055302.1"
                     /note="catalyzes the formation of
                     N-succinyl-2-amino-6-ketopimelate from succinyl-CoA and
                     tetrahydrodipicolinate in the lysine biosynthetic pathway;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
BASE COUNT          668 a         1297 c         1267 g          633 t
ORIGIN      
        1 agcgcggagc aactgtgttc gttgtcaagg ggtgatagcg tgaaggtgct cgttccaagg
       61 gagacccgtc cgcgccatcg cattaaggat gcgaagcatc ttgtgcgagc atgcagtgag
      121 cgcgactttc ttcggcttgc cggccgcgag caaccgttta tagaaggtct tgatcaccgg
      181 gttgtagcga atcgcgctga gcatcgccat atgcaaggcg cagcgcacca ctggacgacc
      241 gccccagatg tgacgtttgc cgcgctcctt ccccgaatcg ttgttcaagg gagccaagcc
      301 gaccagctta gcgaggcggg ctccgttgac ggtgcccaac tctggcaaga atgcgagcag
      361 cgttgcgcag gtaagcgtgc cgacaccctt gatggcgttc aagcgttgaa gctgctcatt
      421 gaagtggcgc tgcaggtgag cgtcgatgtc gcgatccagc ttggcgatct ctttgtccaa
      481 ggtcttgatc acggtttcga tgcttcgttg cagcacgcgc atgggtccgc ccagtcggtt
      541 tctttcggcc aagcgcattg ccagcaactg tccgcgccgc gccaccctgg tctgcagagc
      601 gcccacagcg gcacttggcg ggacgaaggc aatgcccgct tggtccgcct tggccgccaa
      661 cgtcttggcc aagtgggcaa gcacgatggc gtcgatagcg tcggtcttgg ccaagtgccc
      721 catgctgcgt gcgaactcgc gcgcagcgcg cggattgacg accgccaccg gcataccatg
      781 gcccaacaga aacgtggcca gtgcgcgttc gaaaccaccg gtggcctcga gcacaatcag
      841 tccaaggcga cgcccttgca atgcgccgag cagcgcgtgg aatccgctgt cgtcattgct
      901 ggcccgatgc gtgcccggtg ctccatgcag cgcccagtca aaggcttgct gcgccatgtc
      961 aacgcccacg aagtcgagcg gcgacgtcga atcagcggta gcagcgtttg tcatatccca
     1021 tccttgcaaa atgcgaagtg cattcgagta actgttcggg cttggcgaca aacgcaaatg
     1081 acccgcacgc ccctatgctc cgcgacgagc tgtgaactcc agggtagtac gggctgtgca
     1141 ggcatttgca aaaccgctga tcagactgcc aggcgcgatc ccgagtccgc atcgcagcga
     1201 acccggtatc agcatacaag ggtagttcaa tgagcgcgcc ggtaacgcac gccggccggc
     1261 gggcattcct tgcgcgctcg ggcgcgctgg tcgtcacgtt ttcgctcgcg ccgttgcaag
     1321 tcgcggcgca ggaaaaagca ctcgccgcaa agcttcccgg aagcctcgac aaaacgccgc
     1381 tcctcgacgc atggattcgc gtgggcgccg acggcagcgt caccgtgttc accggcaagg
     1441 ccgagctcgg ccagggtgtc aagaccgcgc tgatcgcgct cgccgccgaa gaactcggca
     1501 tcgccccacc agcgatcgtg ctcgtcaccg ccgatacgag ccgcacgccc gacgagggct
     1561 acacggccgg cagcaattcg atgaaggaca gcggaacggc gatccgcagc gccgccgccg
     1621 acgtgcgcga gctgctgatc gagcgtgcgt cggcaaagct cggcgtcccg cccgagcgat
     1681 tgcacgcgca gaatggcaac gtcatcgccg acgacggccg gcgcatcggc ttcggcgacc
     1741 tcgcggcgca aggctacgcc gaggtacgcg cgaagccgaa cgcgaaactc aaggacccgc
     1801 gcagttacac gatagtcggc aggcggctcg cgcgcgtcga catcccggcg aaggtcacgg
     1861 gcggcattgc ctatgtgcag gatctgcgcc cacccgcaat gctgcacgca cgtgtcgtct
     1921 gcccgccgag ccatcgtgcg cggctgcgcg aggccgacgt cgccgctgtc gcgcggatgc
     1981 ccggcgtggt gaaggtcgtg cgcgacggcg gcgtgctcgg cgtggttgcg gcccacgaat
     2041 tcgaggcgat ccgcgcgatg cgcgcgctcg ccgcaactgc ccgctgggac gaggagccgt
     2101 cgttgccgcg gggtgatctt tttgccgcgc tgcaggcgct gccgtcgcag gaaatcgtca
     2161 atgccgatcg acacgctggc gcatcgcaag gcatgcgcac gcttgaagcg acgtacacgc
     2221 gcgcctacca gatgcacggc tccatcggtc cgtcctgcgc gctggcgctt tatcaggacg
     2281 acgcgatgac cgtctggacg cacagccagg gcgtgtatcc gctgcgcgcg tcgatcgccg
     2341 aaatgctgca gatgcaggcc gagcgcgtgc actgcatcca tgccgagggc tccggctgct
     2401 acggtcataa cgcggccgac gacgcggcag cttacgccgc gctattcgct cgcgccgtcc
     2461 cggcgcaccc cattcgcgtg cagtggatgc gcgagcagga gcacgcctgc gagccgtacg
     2521 gaccggcgat gatcgcgaag ctttcgggca gcgtcgacgc gtcgggcagc atcgccgaat
     2581 ggaacgtcga aggctggagc aattcgcaca gcacccgccc gggcggggcg ggcaacctga
     2641 tgcccgcgtg gtatctcgcc acgccgttcg cgcagccggt gccgaaaccg atcccgatgc
     2701 ccgagggggg cggcgatcgc aattcgatcc cgatctatac ggtgccgagc ctgcgcgtcg
     2761 tgcatcattt cattcccgac atgccggtcc gcgtgtcggc gctgcgctcg ctcggcgcgt
     2821 acgtcaacgt gttcgcgatc gagagcttca tcgacgagct cgcgcacgcc gcgggggccg
     2881 atccggtcgc gtatcggatc cgccatctcg gcgatcgacg cgcgcgcgac gtcgtcagcg
     2941 ccgccgctac ccgcttcggc tgggagggtt tcacgcgcaa acccgggcat ggccgcggct
     3001 tcgcctttgc gcgctacaag aacctcgccg cctattgcgc ggtcgccgtc gagctcaccg
     3061 tcgatcacga aaccggccgc gcgcgcgtcg tgcgtgcgac ggcggcgatc gacagcggcc
     3121 aggcggtcga tcccgatggc ctcgcgaacc agatcgaagg tggaatcatc cagtcgctga
     3181 gctggacgct gttcgaggcc gtcaaatacg acgcgacgcg gatcacgagc gtcgactgga
     3241 gcacctaccc gattgcgcgc tttgccgaca ttcccgatcg catcgacgtc cacgtcatcg
     3301 accagcccgg cgaacccttc ctcggcaccg gcgaggcggc acagggcccc acggcagcag
     3361 cgatcgccaa cgcgatcgcc gatgcccgca atgtgcgcct gcgcgacctg ccgctcacgg
     3421 ccgagaaaat caaggtggcg gtcggcgtgt aagatttcgt cctttttgga cgtacgcatg
     3481 cacagtctgc aatcaacgat cgaggccgca tgggaagcgc gcagcgagct ctcgcctgcg
     3541 cgtgcaccgg ttggcgtccg cgacgccgtt gcgcaggtgc tcggcgagct cgacctcggg
     3601 cgccttcgcg ttgccgagaa gatcgacggc gactgggtca cgcatcagtg gatcaagaag
     3661 gcggtgctgc tgtcgttccg ccttgccgac aacgcgccga tcgggctcgc cgggccgcag
     3721 gcgccgttcc gcttctacga caaggtgccg acgaaattcg ccgcctacga cgcggagcgc
     3781 ttcgcgcagg ccgccgttcg cgtcgtcccg ccggcggtga ccatcggcgt caggtgatcg
     3841 agcagctcgg acgcgaggcg cccgg
//
LOCUS       DASSVX010000096         6188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1369817, whole genome shotgun sequence.
ACCESSION   DASSVX010000096 DASSVX010000000
VERSION     DASSVX010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369817"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(341..417)
                     /locus_tag="VFO53_04380"
     tRNA            complement(341..417)
                     /locus_tag="VFO53_04380"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Pro,seq:tgg)
     gene            624..1208
                     /locus_tag="VFO53_04385"
     CDS             624..1208
                     /locus_tag="VFO53_04385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET8875545.1"
                     /translation="MDHRWNVAVLVGSLRRESFNRKMATALARMAPPSLALEIVEIGN
                     LPLYNQDDEANPPPASAAFKSRIEQADAALFVTPEYNRSVPGVLKNAIDIASRPYGKS
                     AWNGKPAAVISVSPGAIGGFGANHALRQSLVFLNMPALQQPEAYIGGATELFDAQGNI
                     AKDSTREFLQKFLAAFAAWIDRNATARGATARTA"
     gene            complement(1245..2765)
                     /gene="lysS"
                     /locus_tag="VFO53_04390"
     CDS             complement(1245..2765)
                     /gene="lysS"
                     /locus_tag="VFO53_04390"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET8875546.1"
                     /translation="MNDRTNASPDNASTDENRIIAERREKLAALRAAGNAYPNDFRRT
                     ALAGDLHAAHDGKDSAALEADAIEVAVAGRMMLKRVMGKACFAALQDMSGRIQLYVTL
                     DGVGEGALDAFKRWDLGDIVGATGTLFKTKTGELSVKCDAVRLLSKALRPLPEKFHGM
                     TDQEQRYRQRHVDLITNLQARDVFVKRSRIVQAIREFFVARGFLEVETPMMHPIPGGA
                     AARPFATHHNALDMPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGISTRHNPEFT
                     MLEFYEAYADYRDLMDLTEVLLREVAQRVCGTTTIVYQGETIDLAQPFPRMTMAEAIR
                     RHGGIEYANVDLSDLATLRRALAPFEVEVFDSDGVGLLQLKLFEATTEEKLLQPTFIV
                     AHPTDVSPLARASDADPAVTDRFELFIARREIANGFSELNDPEDQAARFMAQAKAKEA
                     GDEEAMYYDADYVRALEYGLPPTAGEGIGIDRVVMLLTDAASIRDVILFPHMRPEA"
     gene            complement(2765..3869)
                     /gene="prfB"
                     /locus_tag="VFO53_04395"
     CDS             complement(join(2765..3787,3789..3869))
                     /gene="prfB"
                     /locus_tag="VFO53_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239085.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET8875547.1"
                     /translation="MEPEHVNAIGNALDGLKARTRELRRYLDFDAKATRLATLDRELE
                     DPKIWDNPARAQELGREKKLLDGVVHTLRDLQKEIDDSSELYALARTESDDDTLLAVE
                     ADAGKLERGVADLEFRRMFHNPLDPGNAFVDIQAGSGGTEAQDWAAMLLRMYLRYCER
                     KGYKADVLEESPGEIAGIKSASIKVDGDYAYGYLRTETGVHRLVRKSPFDANARRHTS
                     FASVFVYPEVDESIEVDINPAELRIDTYRASGAGGQHVNKTDSAVRITHLPTNIVVQC
                     QTDRSQHRNRAEAFAMLKSKLYDLELRKRQEQQAKLEDTKTDIGWGHQIRSYVLDQSR
                     IKDLRTSYEVGNTQAVLDGDLDDFISASLKQGV"
     gene            4046..4897
                     /gene="nudC"
                     /locus_tag="VFO53_04400"
     CDS             4046..4897
                     /gene="nudC"
                     /locus_tag="VFO53_04400"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HET8875548.1"
                     /translation="MMHTPEGFTPSHVAPSLVPAASMAFAFAGARLLVAGDEKNPAIP
                     LVGDLERAGLRGARHYLGTLDGVHCVAMLLPDAASPPGYALTGLRALFLRLSEPLLAI
                     AARAFQVIEWDRTHRYCGRCGAPMHDKEGERAKHCGACGYVAYPRISPAMMVLVTRER
                     EVLLARANRFPGAMYSALAGFVEAGESIEDCVHREVREEVGIDVTDLHYFASQSWSFP
                     HSLMIAYTAHYAGGEIVPDASEIADARWFAVDALPDLPSPVSIARRLIDTTVARLRSE
                     SGVTPVG"
     gene            4936..5514
                     /locus_tag="VFO53_04405"
     CDS             4936..5514
                     /locus_tag="VFO53_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8875549.1"
                     /translation="MPPPTIRRFAALFLLASLATAARAQSPPATQDPRQMDETQRRVY
                     TQSLHEAKSLLERKQYADATAALDRLIAERPREPQARFLKGIALTDEGKTDDAIAVFR
                     ALLADFPELPEPRNNLAVLYAQKGEYAQARDELELAVRTAPDYAVAHENLGDVYARLA
                     QVEYEKTVALDKRNRTAAAKLKLIRETPPAPK"
     gene            5529..6134
                     /locus_tag="VFO53_04410"
     CDS             5529..6134
                     /locus_tag="VFO53_04410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET8875550.1"
                     /translation="MVRRLLSVLALGLFAGSALGANPEVELDTTAGTIKLELYPEAAP
                     KTVDNFLHYVKDGHYNGTQFHRVIDGFMIQGGAYTADFSPRPTRPPIVNEAQTSSKAG
                     LHNVPGTIAMARTSNPDSATDQFFINVANNKPLDFRSPDPAGIGYTVFGKVVSGMDVV
                     DKIAKMSKGGRKVQGAAPVDPRSLTTEPVLITSATIVSPTK"
BASE COUNT         1021 a         2112 c         2006 g         1049 t
ORIGIN      
        1 tggcgcggcg cggacggcgc tacgcaggcg aactcggtgt atcactcaac gcgctgctcg
       61 cgatcgcgct gcgcgagtat ctggatgtac gggcgcccac agcgaccgtc tccccatcgt
      121 catcagcgct cgcccaagcc atagcacgtc gcgtgcaagc cggacccgtg cgacaaaagc
      181 ggtgagatcg agcaggtcca ctgacgtgac gacgtttcga ccagttcgtc gttatgagac
      241 aggtcggccc gaacaggcta acgccggttc gggccgaaag tgttacccat gtctccggta
      301 taaaccgtca cctatgtgac cggaatacac ccaggggaat tggtcggggt gagaggattc
      361 gaacctccga catcttgctc ccaaagcaag cgcgctaccg gactgcgcta caccccgaag
      421 cgccgattat gacattcatc ggcggaccgc cggtcgcgct cgttgcccga ttctgcggcg
      481 ttgcggcacg attcaacggc gtcgcggccg cactgcattg ccaatcggaa cttcggcgcc
      541 tgcgatgctc tcgtactcca cggggcgcgc ggcgcagtgg gaaagcgcgc tcgtcgacgc
      601 ccgcaacaaa cgaggaacag ccgatggatc atcgatggaa cgtcgcagtg ctcgttggaa
      661 gcctgcgcag ggaatcgttc aaccgcaaga tggcgacggc gttggccagg atggcgccgc
      721 cgtcgctcgc gctcgaaatc gtcgagatcg gcaatcttcc gctctacaac caggacgacg
      781 aggccaatcc gccgcccgct tcggcggcgt tcaagtcacg catcgagcag gcggacgccg
      841 cgctgttcgt cacgcccgag tacaaccgct cggtccccgg cgtgctgaag aatgcgatcg
      901 acatcgcctc gcgtccgtac ggcaaaagcg cctggaacgg caagccggcc gcggtgatca
      961 gcgtatcgcc gggtgcgatc ggcgggtttg gcgcgaacca cgcgctgcgc cagtcgctgg
     1021 tgttcctcaa catgcccgcg ctgcagcagc ccgaggcata catcggcggt gccaccgaac
     1081 tcttcgatgc gcaggggaac atcgccaagg actcgacgcg cgagttcctg cagaagttcc
     1141 tcgctgcctt cgctgcgtgg atcgatcgca acgcaacggc gcgcggcgct accgcccgga
     1201 ccgcctgacc agcgccagaa ccgcacggat gccgcgcgca cccgctaagc ctccggacgc
     1261 atgtgcggga acaggatcac gtcgcggatc gatgcggcgt cggtgagcag catgaccacc
     1321 cggtcgatgc cgataccctc gcccgcggtc ggcggcagcc cgtattcgag cgcgcgcacg
     1381 taatcggcgt cgtaatacat cgcctcctcg tcgccggctt ccttggcctt cgcctgcgcc
     1441 atgaagcgcg ccgcctgatc ctccggatcg ttgagctcgg agaaaccgtt cgcgatctcg
     1501 cgtcgcgcga tgaaaagctc gaagcgatcg gtgaccgcgg gatcggcgtc gctcgcgcgc
     1561 gcaagcggcg acacgtcggt cggatgcgcg acgatgaacg tcggctgcag gagcttttcc
     1621 tcggtcgtcg cctcgaagag cttgagctgc agcaagccga cgccgtcgct gtcgaacacc
     1681 tcgacctcga acggcgccag cgcgcgccgc aatgtcgcga ggtctgacag atcgacgttg
     1741 gcgtactcga tgccgccgtg gcgccggatc gcctcggcca tcgtcatgcg cgggaacggc
     1801 tgcgcgaggt cgatcgtttc accctggtac acgatcgtcg tcgttccgca cacgcgctgc
     1861 gccacctcgc gcagcagcac ctccgtcagg tccatgaggt cgcggtagtc ggcgtaggcc
     1921 tcgtagaact cgagcatcgt gaactcgggg ttgtgccgtg tcgagatgcc ctcgttgcga
     1981 aagctgcgat tgatctcgaa cacgcgctcg agtccgccga cgacgagacg cttgagatag
     2041 agctcgggcg cgatgcgcag gtacagcggc atgtcgagcg cgttgtgatg cgtcgcgaac
     2101 gggcgcgccg cggcgccgcc ggggatcgga tgcatcatcg gcgtctcgac ctcgaggaag
     2161 ccgcgggcga cgaagaactc gcggatcgct tgcacgatgc gcgagcgctt gacgaagacg
     2221 tcgcgcgcct gcaggttggt gatgagatcg acgtgccgct ggcggtagcg ctgctcctga
     2281 tcggtcatgc cgtgaaactt ctcgggcagc ggccgcagcg ccttcgagag cagccggacc
     2341 gcatcgcact tgacgctgag ctcgccggtc ttcgtcttga acagcgttcc cgtcgcgccg
     2401 acgatgtcgc ccaggtccca gcgcttgaac gcatcgagcg cgccttcgcc gacgccgtcg
     2461 agcgtgacat aaagctggat gcgcccggac atgtcctgca gcgccgcgaa gcacgccttg
     2521 cccatcacgc gcttcagcat catccgcccg gcaacggcga cttcgatcgc gtcggcttcg
     2581 agtgccgcgc tgtccttgcc atcgtgagcg gcgtgcaagt cgcctgccag cgcggtgcgc
     2641 cggaaatcgt tcggatacgc gttgcccgct gcacgcagcg cagcaagctt ctcgcggcgc
     2701 tcggcaatga tgcggttctc gtcggtgctt gcgttgtccg gcgaagcgtt cgtcctgtcg
     2761 ttcactacac cccctgcttg aggctcgcag aaatgaaatc gtcgaggtcg ccgtcgagca
     2821 cggcctgcgt attgcccacc tcgtagctgg tgcgcaggtc cttgatgcgc gactggtcga
     2881 gcacgtacga gcggatctgg tgtccccagc cgatgtcggt cttcgtatcc tcgagcttcg
     2941 cctgctgctc ctggcgcttg cgcaactcga ggtcgtagag cttcgacttc agcatcgcga
     3001 acgcctcggc gcggttgcgg tgctgcgagc ggtcggtttg gcactgcacg acgatgttcg
     3061 tcggcagatg ggtgatgcgc accgccgaat cggtcttgtt cacgtgctgc ccgcccgcgc
     3121 cggatgcgcg gtaggtatcg atgcgcagct cggcagggtt gatgtcgacc tcgatcgatt
     3181 cgtcgacctc gggatagacg aacacgctcg cgaacgacgt atgccgccgc gcgttggcat
     3241 cgaacgggct cttgcgcacc agccggtgca cgccggtttc ggtgcgcagg tagccgtagg
     3301 cgtagtcgcc gtcgaccttg atcgatgcgc tcttgatgcc agcgatttcg cccggcgact
     3361 cctcgagcac gtcggccttg tagcccttgc gctcgcagta gcggaggtac atccgcagca
     3421 gcatcgccgc ccagtcctgc gcctcggtgc caccgctgcc ggcctggatg tcgacgaacg
     3481 cgttgccggg gtcgagcgga ttgtggaaca tccggcggaa ctcgagatcg gcgacgccgc
     3541 gctcgagctt gccggcgtct gcctcgacgg cgagcagcgt atcgtcgtcg gattcggtgc
     3601 gcgcgagcgc atacagctcg ctgctgtcgt cgatttcctt ttgcaggtca cgaagcgtgt
     3661 gcaccacgcc gtcgagcagc ttcttctccc ggccgagctc ctgcgcgcgc gccgggttgt
     3721 cccagatctt cggatcctcg agctcgcggt ctagcgtggc aagccgtgtc gccttcgcat
     3781 cgaagtcaaa gatacctccg aagctcgcgc gtgcgcgcct tcaggccgtc gagggcgttg
     3841 ccgatcgcat tgacgtgttc gggttccatg ccgcttgcct tcctcgatgg tgaaccgtgc
     3901 attatagcgg cgggcgtgcg tgcgtagcgg cgggcgcgcg tacgggccgg cgcagcacgc
     3961 tgcgcttgtc gcacctcggg ctgcagcgcc cgccgcacgc actaccatga ccgccattcg
     4021 agaccgaaca cgccgcccgc caacgatgat gcatacgccc gaaggcttca cgccgtcgca
     4081 tgtcgcgccg tcgctcgtcc ccgccgcgtc gatggctttc gcgttcgccg gcgcaaggct
     4141 gctggtcgcc ggtgacgaga aaaaccccgc cattccgctg gtgggcgacc tcgagcgcgc
     4201 gggccttcgc ggcgcacgcc actacctcgg gacgctcgac ggcgtccact gcgtggcgat
     4261 gctgctgccc gacgccgctt cgccgcccgg atacgcgctc acggggctgc gcgcgctttt
     4321 cctgcggctg tccgagccgc tgctcgcgat cgcggcgcgc gcgttccagg tgatcgagtg
     4381 ggaccgcacg catcgctact gcggacgctg cggcgccccg atgcacgaca aggaaggcga
     4441 gcgcgcgaag cactgcggcg cctgcggtta cgtcgcctac ccgcgtatct cgccggcgat
     4501 gatggtgctc gtcacgcgcg aacgcgaggt gctgctcgcg cgtgcgaacc gcttcccggg
     4561 tgccatgtac agcgcgctgg ccggcttcgt cgaggcgggc gagtcgatcg aggactgcgt
     4621 ccaccgcgaa gtgcgcgagg aggtcggcat cgacgtcacc gacctgcact acttcgcgag
     4681 ccagtcgtgg tcgttcccgc actcgctgat gatcgcgtac accgcgcatt acgcgggtgg
     4741 cgagatcgtg cccgacgcgt cggaaatcgc cgacgcccgc tggttcgctg tcgatgcgct
     4801 gcccgacctg ccgagcccgg tatcgattgc gcgccggctg atcgacacca ccgtggcccg
     4861 gttgcgctcg gaatcgggcg tcactccggt gggttagaat tcacgcacgc gaaacggcac
     4921 tcgccgcccg ccgccatgcc cccgccaacc attcgccgat tcgcagcact gttcctgctc
     4981 gcatcgcttg cgacggccgc ccgcgcccag tcgccgccgg cgacgcagga cccgcgccag
     5041 atggacgaga cgcagcgcag ggtctacacg caatcgctgc acgaagcgaa gagcctgctc
     5101 gagcggaagc agtacgccga cgccacggcg gcgctcgacc ggctgatcgc cgagcggcca
     5161 cgcgagccgc aggcgcgctt cctcaagggg atcgcactca ccgacgaggg caagaccgac
     5221 gacgcgatcg cggtctttcg cgcgctcctc gccgattttc cggagctccc cgagccgcgc
     5281 aacaatctcg cggtgctgta tgcgcaaaag ggcgagtatg cgcaggcacg cgacgagctc
     5341 gagctcgcgg tgcgcacggc ccccgactac gcggtcgcgc acgagaacct cggcgacgtc
     5401 tatgcgcgcc ttgcgcaggt ggaatacgag aagacggttg cgctcgacaa gcgcaatcgc
     5461 accgcggccg ccaagctcaa gctgatccgc gagacacccc ccgcccccaa atgacaagga
     5521 gccgctcgat ggttcgcaga ttgttgtccg tcctcgcgct gggattgttc gcggggtccg
     5581 cgctcggcgc caatcccgag gtcgagctcg acacgaccgc cggcacgatc aagctcgagc
     5641 tctatcccga agctgcaccg aagacggtcg acaacttcct tcattacgtc aaggacggcc
     5701 attacaacgg cacgcagttc caccgcgtga tcgacggctt catgatccag ggcggtgcct
     5761 acacggccga tttcagtccg cggcccacgc gtccgccgat cgtcaacgag gcgcagacga
     5821 gcagcaaggc cgggctgcac aacgtgcccg ggacgatcgc gatggcgcgc acgtccaatc
     5881 ccgattcggc gaccgaccag ttcttcatca acgtcgcgaa caacaagccg ctcgatttcc
     5941 gctcgcccga tccggccggc atcggctata cggtattcgg caaggtcgtg tccggcatgg
     6001 acgtcgtcga caagatcgcg aagatgagca agggcgggcg caaggtgcaa ggtgcggcac
     6061 cggtcgatcc ccgctcgctc acgaccgagc ccgtgctcat cacgtcggct acgatcgtat
     6121 cccccacgaa gtaggaccgc ccgcgagggc ccggaggcaa ggtcatgagt gaagtggtcc
     6181 tgcatacg
//
LOCUS       DASSVX010000097         9931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_336679, whole genome shotgun sequence.
ACCESSION   DASSVX010000097 DASSVX010000000
VERSION     DASSVX010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336679"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFO53_04415"
     CDS             complement(<1..209)
                     /locus_tag="VFO53_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875551.1"
                     /translation="MFRHRDSFVSCVAARAALPGRPAAPRFVSFVSPAGPGARAGRIP
                     FMLSQTIRPSLAAMSPNTTILVVDD"
     gene            353..919
                     /locus_tag="VFO53_04420"
     CDS             353..919
                     /locus_tag="VFO53_04420"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HET8875552.1"
                     /translation="MYFGVDPIRSLTGEPSCTRKRSAVPVHDESRNGAASSTPATMRW
                     TTAAAGNDASCGPAPRTGGRFAPPRWEILFESGDGYVASPDEPMFGWSTSYEVFQAAR
                     MRRAAVMAALLHAAGRTVREWFRGVVERYQRRRQSRSIYHALRALDDRTLRDLGFHRC
                     EIGSVAAEAAGEAELTRMHVRRVPRAPS"
     gene            complement(948..1907)
                     /locus_tag="VFO53_04425"
     CDS             complement(948..1907)
                     /locus_tag="VFO53_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HET8875553.1"
                     /translation="MNRIRVVDSHTGGEPTRVVVEGGPDLGHGPLAERRERFRSAHDH
                     IRCGIVNEPRGSDAVVGALLCAPVDPACAAGVIFFNNAGCIGMCGHGTIGLAVTLAHL
                     GRIAPGVHRIETPVGAVRVELLDRHRVTLDNVPSYRHAANVEVPVPGFGTVKGDVAWG
                     GNWFFIVESPGLDIVASNVGRLTAFARALRAGLAAKRIRGAEGAEVDHVEVYAPLATA
                     ARGSRNFVLCPGNAYDRSPCGTGTSAKLACLHADGALRPGETWRQESVTGSVFDASFT
                     LDAAAPGRIVPRITGSAYVTGESALVFDEDDPLRWGMPRHDAS"
     gene            complement(1939..3864)
                     /locus_tag="VFO53_04430"
     CDS             complement(1939..3864)
                     /locus_tag="VFO53_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET8875554.1"
                     /translation="MRRSIATVSMSGTLRDKLEAIAAARFDGYELFESDLLYYHASPA
                     EVGALAADLGLACELYQPFRDFEGSDSAAFERSLERAERKFDVMHALGCRLMLVCSNT
                     RADMIADEALMADQLHALAERAARRNLRVGYEALAWGRVVNRFAQAWSIVRRANHPHL
                     GLILDSFHTLSLRDDPAGIASIPGDRIFFLQMADAPWLTLDVTNWARHHRNFPGQGQL
                     DVVRFFEQALLAGYSGPLSLEIFNDVFRQTPNRRTATDAMRSLLFLESEVRERLQADA
                     AGAGAPVAKRALERIDLFDAPAAARFSGVAFLEFAVDEAAAAELAAWFERLGFRRAGK
                     HRSKEVTLYRQGEVNVILNADPALPARDFFAARGPSVCALGLWTDDPVRAQNRATALQ
                     SARAEGRVGPSEVPIPAIVAPGRTIIYFVDEALAPDRLFEIDFDLDATGQNAPGPVRR
                     VDHVAFGLPLGELDTWVLFCRAVLGMEVGDSLELADPFGLVRSAGVATPDRRLRFVLN
                     VSLGEKTGNARTVNASGGSATVHHLGVAVDDALAAAESLRRSGMPLVAVSANYYDDLL
                     ARTDLDVATLDAMRHASVLYDSAGGGEYLHGYTPVFADRFFVEIVERRGGYDAYGALN
                     APARMVSQAQARSPRRA"
     gene            complement(3919..4599)
                     /locus_tag="VFO53_04435"
     CDS             complement(3919..4599)
                     /locus_tag="VFO53_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018897891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8875555.1"
                     /translation="MIESATGAATLSDRVYSRIRSDVILCRLAPGQRLTLDRMRDVYG
                     TSIGTLREVLNRLASEGFVAAEGARGFEVAPTSVENLREVAAVRQLLECHALRESFAA
                     GDIEWEGRIVAAHHKLAILERRMVEGDHSESEAWKRYDAAFHNALISACGSKLLLEMH
                     AAIYDKYLRYLVLADVFRGEVAASEHRRLLEYALARNSEAAQRMLVTHVRDCVAHIIG
                     KGLVHTSA"
     gene            4783..5775
                     /gene="dctP"
                     /locus_tag="VFO53_04440"
     CDS             4783..5775
                     /gene="dctP"
                     /locus_tag="VFO53_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET8875556.1"
                     /translation="MNPASPLDRRTFLKAGASLVAAGAVPAALAQGKTKLRFSSAFTE
                     QDLRAEAYKNFAAAIKDSFEFEPYWNNTLFKQGTELVALQRGNLEMANLAPQDISKQL
                     PAWSLMTSAYLFRDPDHLRKTFRSDIGREFMKMARDKLDIQIITPVYFGARHVNLKPD
                     KTIRTPQDLAGIKLRMPPGEFWQFLGESIGVNPTPVAFAEVYTALQTGAIDGQDNPLL
                     LSKLMKFYEVTTQFVLTSHVIGYDVMAVSTKTWDAMSPAVQAKFQAAAEKAIDDYTTK
                     FNGQEKDVVEFFKAQGKKVYTPDVNAFRTYAQKKYVDKYGQDWPKGALERINAL"
     gene            5783..6292
                     /locus_tag="VFO53_04445"
     CDS             5783..6292
                     /locus_tag="VFO53_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HET8875557.1"
                     /translation="MRLSPGRRLRKLADHVAVALMATMFVSFLLQIFFRYVLNRPLGW
                     TEEVTVLCWLWVVLWSASFILSDREEVRFDIVYGIVPRRLQRGFTVVSSIALIVLLAW
                     SLPATWKYVVFMKREHSAYLHMRFDALYSIYLLFAVACIVNHVRLVWRTLRATEPAES
                     TIEQAGDFR"
     gene            6289..7587
                     /locus_tag="VFO53_04450"
     CDS             6289..7587
                     /locus_tag="VFO53_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET8875558.1"
                     /translation="MTLSPFTLCIGVLVLLAVLGLPIGLAMIGASIFYLVSAGLDAGT
                     AAEQILNGLYSSYVLLAVPLFILAADLMNTGSLTDRLLRFCLVLVGRFRGGLGHVNVV
                     SNVIFAGMSGSAIADAVGIGRVIIGMMTREGRYPVSYAAAITACAAIIGPIIPPSIPM
                     VVYALVADTSIGYLFLGGVVPGLMLGVAFMVMNTVIARRRHYPVEPSVPLREIPKIAL
                     DAFPALLLPVILLFGIYGGVMTPTEGAAAAAAYALLAAAVIYRAMSLRQLYGVLLSSA
                     KATTSVGMLIAGALVFNYVVTIENVPASVAHFIAGFHLSQVAFLLLINAILLVLGCLL
                     EGTTILLVIVPIMIPTAKVLGVDLVHFGVVVVVNVMIGLLTPPFGLLLFIVANMTSQP
                     LARIVRECVPFIAAAIVVLAVITFVPETTLWLPRMFGYKG"
     gene            7636..8076
                     /locus_tag="VFO53_04455"
     CDS             7636..8076
                     /locus_tag="VFO53_04455"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET8875559.1"
                     /translation="MNDAGTKPLHILNGPNLNRLGQREPSIYGTASLAQIEAQCRDEA
                     GACPIVFRQTNSEAQLVDWIHEAIDEAGAIIINPAAYSFTSIAILDALKMFPGPIIEV
                     HISNIHRREPHYHRSYVSLAATAVIAGLGPAGYRLAVRALREMA"
     gene            8188..8370
                     /locus_tag="VFO53_04460"
     CDS             8188..8370
                     /locus_tag="VFO53_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HET8875560.1"
                     /translation="MADNLNERGAGDRTRINVSQPHELSYWSQKLGVTEEQLRTAVQK
                     AGPMADDVRRHLGKAA"
     gene            8372..8557
                     /locus_tag="VFO53_04465"
     CDS             8372..8557
                     /locus_tag="VFO53_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019585678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transport regulator"
                     /protein_id="HET8875561.1"
                     /translation="MPYKAIADLPEAQTDQYNEHQKRAFLEAFNNAYDEYHGDEHRAF
                     AVAHAAAKRAGERPGPG"
     gene            complement(8567..9439)
                     /locus_tag="VFO53_04470"
     CDS             complement(8567..9439)
                     /locus_tag="VFO53_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875562.1"
                     /translation="MRSVHELGVPADTAVTQPASSRPRRLRERIGKRVRELAAPDHVF
                     PVLAIILFFVVWEIVCRVFSVPVFILPKPSEFLGVLITKWPAILPNAVQTLYTTIVGF
                     ALGVLVGAAIGACIGLSRFVYRVTYPLLIGFYSVPKIAVVPILVIWFGIGTIPAILTA
                     AIMCLFPVVVNVATGLATTEPELEDVLRSLGASKLDILRRVGLPRSMPYFFASLKIAV
                     SLAFVGTVVAETVAANIGIGSMMILASSTFDVPLVFAGVFLLAVLGVAMYAVFALIER
                     RVTFWARRNADVAV"
     gene            complement(9451..>9931)
                     /locus_tag="VFO53_04475"
     CDS             complement(9451..>9931)
                     /locus_tag="VFO53_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8875563.1"
                     /translation="IVEPHRSQFRAKKDAYRAMAQSLLGEVGLGNFADKYPWELSGGM
                     LQRASLCRALIHDPRLLLLDEPFGALDAFTREDLWQVLQKLWMERNPTVILVTHDLRE
                     AVFLADTVHVMSARPGRVLTTHRVDIRRPRTLDDLFTDQASQLVHELRRHIAHDRPV"
BASE COUNT         1709 a         3330 c         3296 g         1596 t
ORIGIN      
        1 tcgtcgtcga ccaccaggat cgtcgtgttc gggctcattg ctgccaggga cggtcggatc
       61 gtttgcgaca gcataaaggg aattcgcccg gcgcgcgccc cggggccggc gggtgaaaca
      121 aacgaaacaa atcggggagc agccggcctt cccggcagcg cggctcgcgc cgctacacag
      181 gaaacaaaac tgtcgcggtg gcgaaacatg catgccgcat tcgctcacta gacttcatct
      241 cactctcgca cgcgggaggt ggcgcaagga agcggcagcc gatccccggc cggcacttcc
      301 gggcgaaaac gtgccattgc gtcaatcgat cttttcgaaa ggaacgaccg tgatgtactt
      361 cggagtcgac ccgatccgct cgctgactgg cgaaccctcg tgcacgcgca aacgcagcgc
      421 cgtcccggtg catgacgaaa gccgcaacgg cgccgcaagc agcacgccgg caacgatgcg
      481 atggacgacg gcggcggcgg gcaacgacgc gagctgcgga ccggcgccgc gcaccggcgg
      541 gcggttcgca ccgccgcgat gggaaattct gttcgaatcg ggcgacggtt acgtggcaag
      601 tcccgacgag cccatgttcg gatggtcgac gagctacgag gtgttccagg cggctcgcat
      661 gcgtcgagcc gccgtcatgg cggccctcct gcatgccgcg gggcgcacgg tccgtgaatg
      721 gttccgcggc gtagtggagc gctaccagcg ccggcggcaa tcgcggtcga tctaccacgc
      781 actgcgcgcg ctcgacgacc gcacgctgcg cgatctcggg ttccaccgct gcgaaatcgg
      841 atcggtcgcc gcagaagccg cgggagaggc cgagctcacc cggatgcacg tcaggcgagt
      901 gccgcgcgcg ccttcgtaga ttcggccgcg ctgcaaaacg gcgggactta cgacgcgtcg
      961 tggcgcggca tgccccagcg cagcggatcg tcctcgtcga agacgagcgc cgattcgccg
     1021 gtgacgtacg cgcttccggt gatccgcggc acgatgcggc cgggcgcggc ggcgtcgagc
     1081 gtgaacgacg cgtcgaacac gcttccggtc acgctttcct ggcgccatgt ctcgcccgga
     1141 cgcagcgcgc cgtccgcatg cagacaggcg agcttcgcgc tcgtccccgt cccgcagggc
     1201 gagcgatcgt aggcgttgcc cgggcacagc acgaagttgc ggctgccgcg cgccgccgtc
     1261 gcgagcggcg catacacctc gacgtggtcg acctcggcgc cctcggcccc gcggatacgc
     1321 ttcgccgcga ggcccgctcg gagtgcccgg gcgaacgccg tcaggcgccc gacgttcgac
     1381 gcaacgatgt cgagacccgg ggattcgacg atgaagaacc agttgccgcc ccaggcgacg
     1441 tcgcccttca ccgtgccgaa cccgggcacc ggcacttcga cgttcgccgc gtgccgatag
     1501 ctcggcacgt tatcgagcgt gacccggtgg cggtcgagca attcaacgcg caccgcgccg
     1561 accggcgttt cgatgcggtg cacgccgggc gcgatgcggc cgagatgcgc gagcgtcacc
     1621 gcaagcccga tcgtgccgtg gccgcacatc ccgatgcagc cggcattgtt gaagaagatc
     1681 acacccgccg cgcacgccgg atcgacgggc gcgcagagca gcgcgccgac gacggcatcc
     1741 gagccccgcg gctcgttgac gatgccgcag cggatgtggt cgtgcgcgct tcgaaatcgc
     1801 tcgcggcgct cggcgagcgg gccgtggccg agatccggcc cgccctcgac gaccacgcgc
     1861 gtcggctcgc cgccggtgtg cgagtcgacg acgcgtatgc ggttcatgcg gctctcgcag
     1921 gacgtggcac cgcgccggtc acgcgcgtcg cggcgaccgc gcctgggcct gcgaaaccat
     1981 gcgcgccggc gcgttcagcg ctccgtacgc gtcgtatccg ccgcgccgct ccacgatctc
     2041 gacgaagaaa cgatcggcga acaccggcgt gtaaccgtgc agatattcgc cgccgcccgc
     2101 gctgtcgtag agaaccgatg cgtgccgcat cgcgtcgagc gtagccacgt cgagatcggt
     2161 gcgcgcgaga agatcgtcgt agtagttcgc cgagaccgcg acgagcggca tgcccgatcg
     2221 gcgcagcgat tcggccgcgg ccagcgcgtc gtcgacggca acgcccagat gatgcacggt
     2281 ggcgcttccg ccggacgcgt tcacggtacg cgcgttgccg gtcttctcgc ccagcgacac
     2341 gttgagcacg aagcggagcc gccggtcggg cgttgcaacg cccgcgctgc ggaccaggcc
     2401 gaacggatcg gcaagctcga ggctgtcgcc aacctccatg cccagaacgg cgcgacagaa
     2461 cagcacccat gtgtcgagct cgcccagcgg caggccgaac gcgacatgat cgacgcggcg
     2521 cacgggtccg ggtgcattct gcccggttgc gtcgagatcg aagtcgattt cgaacaggcg
     2581 atcgggcgcg agcgcctcgt cgacgaaata gatgatggtg cggcccggcg cgacgatcgc
     2641 cggaatcggc acctcgctcg gccccacgcg gccctcggcg cgggcgcttt gcaatgcggt
     2701 ggcgcggttt tgtgcacgca ccgggtcgtc ggtccacagg ccgagcgcac acaccgacgg
     2761 gccgcgggcg gcgaagaagt cccgcgcagg caatgccggg tcggcattga ggatcacgtt
     2821 gacctcgcct tgccggtaaa gcgtcacctc cttcgaacga tgctttccgg cgcggcgaaa
     2881 cccgagccgt tcgaaccacg cggcgagctc ggccgcggcg gcttcgtcga cggcgaactc
     2941 gaggaacgca acccccgaga atcgagcggc tgccggtgca tcgaagaggt cgatgcgctc
     3001 gagcgcgcgt tttgccaccg gcgcgccggc gcccgcggca tcggcttgca ggcgctcgcg
     3061 cacctcgctt tcgaggaaca ggagcgagcg catcgcgtcg gtggccgtgc gccggttcgg
     3121 cgtctggcgg aacacgtcgt tgaagatctc gagcgacagc ggcccgctgt agccggcgag
     3181 cagcgcctgc tcgaagaagc gcacgacgtc gagctgcccc tgtcccggaa agttgcggtg
     3241 atgccgtgcc cagttcgtga cgtcgagtgt gagccacggc gcgtcggcca tctgcaggaa
     3301 aaagatccgg tcgccgggaa tgctcgcgat gccggccgga tcgtcgcgca gcgacagcgt
     3361 atgaaagctg tcgaggatca gtccgaggtg cggatggttc gcacgccgca cgatcgacca
     3421 cgcctgcgcg aagcggttga ccacgcggcc ccacgcgagc gcctcgtaac cgacgcggag
     3481 gttgcgccgc gccgcacgtt ccgccagcgc atggagttga tcggccatca gcgcctcgtc
     3541 ggcgatcatg tcggcgcgcg tgttcgaaca gacgagcatc agccggcatc cgagcgcatg
     3601 catcacgtcg aatttgcgct cggcgcgctc gagactgcgc tcgaacgcag cgctatccga
     3661 gccttcgaag tcgcggaagg gctggtagag ctcgcaggcg aggccgaggt cggcggcaag
     3721 cgcaccgact tcggccggcg acgcgtggta gtacaacaga tcgctctcga aaagctcgta
     3781 gccgtcgaag cgcgccgcgg cgatcgcctc gagcttgtcg cgcagcgtgc cgctcatcga
     3841 aacggtggcg atcgatcggc gcatggaaga atcccgggga gtcggaggcg gacgcgcgac
     3901 gcgtgcgtgg cgcggaagct aggcgctcgt gtgcacgagg cccttgccga tgatatgcgc
     3961 aacgcaatcc cgcacgtgcg tcaccagcat ccgctgcgcc gcttccgaat tgcgggcgag
     4021 cgcgtattcc agcagcctgc ggtgctcgct ggcggcgacc tcgccgcgga agacgtccgc
     4081 cagcaccagg tagcgaagat acttgtcgta gatcgccgcg tgcatttcga gcagcagctt
     4141 cgatccgcaa gccgagatca acgcgttgtg gaacgcggcg tcgtatcgct tccacgcctc
     4201 gctttcggaa tggtcgcctt cgaccatccg cctttcgagt atcgcgagct tgtggtgcgc
     4261 ggcgacgatc ctgccttccc attcgatgtc gcctgccgcg aacgactcgc gcagcgcgtg
     4321 gcattcgagg agctggcgaa ccgcggccac ctcgcgcagg ttctcgacgg atgtcggcgc
     4381 aacctcgaac ccgcgcgcgc cttcggcggc aacgaagccc tcggacgcga gccggttcag
     4441 cacctcgcgc agcgtgccga tgctcgtccc gtagacatcg cgcatccggt cgagcgtcag
     4501 gcgctgaccg ggtgcgaggc ggcacaggat cacgtcgctg cgaatgcggc tgtaaaccct
     4561 gtcgctgagc gtcgcggcgc cggttgccga ttcaatcatt gctttggtcg atgtaacgtg
     4621 gatgagtcga aagcgcgcga tgtagacgct cgcgcgccga tcataccggc cgaacgcgat
     4681 gcttggcagg ctcggacgat cttgctactc tccgatgcct cggccattat ccaataatcc
     4741 gatcgggccg gccagacgcc cgatcctcat tcaggaggtg tcatgaatcc ggcttcgccg
     4801 ctcgaccgcc gcacgttcct caaggccggt gcttccctcg tcgccgccgg cgccgtgccg
     4861 gcagcactcg cacaaggcaa gacgaagttg cgcttctcga gcgcgttcac cgagcaggac
     4921 ctgcgcgccg aggcatacaa gaattttgcc gccgcgatca aggacagctt cgagttcgag
     4981 ccgtactgga acaacacgct gttcaagcag ggcaccgagc tcgtcgcgct gcagcgcggc
     5041 aacctcgaga tggccaacct cgcgccgcag gacatttcga agcagctgcc ggcgtggtcg
     5101 ctgatgacgt cggcgtacct gttccgcgat cccgatcatc tcaggaagac gtttcgcagc
     5161 gatatcgggc gcgagttcat gaagatggcg cgcgacaagc tcgacatcca gatcatcacg
     5221 ccggtttact tcggcgcacg tcacgtgaac ctgaagcccg acaagaccat ccgcacgccg
     5281 caggatctcg ccggcatcaa gctgcgcatg ccgccgggtg agttctggca gttcctgggc
     5341 gaatcgatcg gcgtcaatcc cacgccggtc gcgttcgccg aggtgtacac cgcgctgcag
     5401 acgggcgcca tcgacggcca ggacaatccg ctgctgctct cgaagctgat gaagttctac
     5461 gaggtgacga cgcagttcgt gctgacgagc cacgtgatcg gatacgacgt gatggcggtg
     5521 tcgacgaaga cgtgggacgc gatgtcgccg gcggtgcagg cgaagttcca ggcggcagcc
     5581 gagaaggcga tcgacgacta cacgaccaag ttcaacggac aggagaagga cgtcgtcgag
     5641 ttcttcaagg cacagggcaa gaaggtgtac acgcccgacg tcaatgcctt ccgcacgtac
     5701 gcgcagaaga aatacgtcga caagtacggg caggactggc cgaagggcgc gctcgagcgc
     5761 atcaacgcgc tgtgaggcgt gaatgcgcct gagcccgggg cgcaggctgc gcaagctcgc
     5821 ggaccacgtc gcggtcgcat tgatggcgac gatgttcgtg agcttcctgc tgcagatctt
     5881 ctttcgctat gtcctgaacc gcccgctcgg ctggaccgag gaggtcacgg tcctgtgctg
     5941 gctgtgggtc gtgctgtgga gcgcctcgtt catcctgtcc gaccgggaag aggtgcgttt
     6001 cgacattgtc tacggcatcg ttccgcgccg cctccagcgc ggcttcaccg tcgtctccag
     6061 catcgcgctg atcgtgctgc tcgcctggtc gcttcccgca acgtggaagt acgtcgtctt
     6121 catgaagcgc gagcactcgg cgtacctgca catgcgcttc gacgcgctgt attcgatcta
     6181 cctgctgttc gccgttgcat gcatcgtcaa tcacgtgcgc ctcgtgtggc gcacgctgcg
     6241 cgcgaccgag cccgccgaat cgacgatcga acaggcgggt gatttccggt gacgctgtcg
     6301 ccgttcacgc tgtgcatcgg cgtgctggtc ctgcttgcgg tgctcggact gccgatcggc
     6361 ctcgcgatga tcggcgcgtc gatcttctac ctggtcagcg ccgggctcga cgccggtacc
     6421 gcagccgagc agatcctgaa cggcctctac agcagctacg tgctgctcgc ggtgccgctg
     6481 ttcatcctcg cggccgacct gatgaacacc ggcagcctca ccgaccggct gctcagattc
     6541 tgcctcgtcc tggtcggccg ctttcgcggc ggcctcggtc acgtcaatgt cgtttcgaac
     6601 gtgatcttcg ccggcatgtc gggctcggcg atcgccgacg ccgtggggat cggccgcgtg
     6661 atcatcggca tgatgacgcg cgagggccgc tatccggttt cgtatgccgc cgcgatcacc
     6721 gcgtgtgccg cgatcatcgg tccgatcatt ccgccgtcga ttccgatggt cgtgtatgcg
     6781 ctcgtcgccg acacgtcgat cggctacctg ttcctcggcg gtgtcgttcc cggcctcatg
     6841 ctgggcgtcg cgttcatggt gatgaacacg gtgattgcgc gccggcggca ctatccggtc
     6901 gagccttcgg tgccactgcg cgaaatcccg aagatcgcgc tggatgcatt tccggcgctg
     6961 ctgctgccgg tcatcctgct gttcggcatc tacggcggcg tgatgacgcc caccgagggc
     7021 gcggcagcag cggccgcgta tgcgctcctg gcggccgcgg tgatttatcg cgcgatgagc
     7081 ctgcgccagc tttacggcgt gctgctgtcg agcgcgaagg cgacgacgtc ggtcggcatg
     7141 ctgatcgcag gcgcgctggt cttcaactac gtcgtcacga tcgagaacgt gccggcgtcg
     7201 gtcgcgcact tcatcgccgg ctttcacctg tcgcaggtcg cgttcctgct gctgatcaac
     7261 gcgattctgc tggtcctggg ctgcctgctc gagggcacga cgatcctgct cgtgatcgtt
     7321 ccgatcatga tcccgacggc gaaggttctc ggcgtcgacc tcgtccattt cggcgtggtc
     7381 gtcgtcgtca acgtgatgat cggcctcctg acgccgccgt tcggcctgct gctgttcatc
     7441 gtcgcgaaca tgacctcgca gccgctcgcc agaatcgtac gcgaatgcgt gccgttcatc
     7501 gccgcggcga tcgtcgtgct ggcggtgatc acgttcgtgc ccgagaccac gctgtggctg
     7561 ccgcgcatgt tcggctacaa gggttgatcg tcgacattcg gcgagaggcc cgttccgcga
     7621 attgacatcg acaccatgaa cgacgccggc accaaaccgc tccatatcct caatggcccc
     7681 aaccttaacc gcctcggcca gcgcgagcca tcgatttacg gcaccgcgag cctcgcgcaa
     7741 atcgaagcgc agtgccgcga cgaagccggt gcgtgcccga tcgtgttccg gcagacgaac
     7801 agcgaagcgc agctcgtcga ctggatccac gaggcgatcg acgaggccgg cgcgatcatc
     7861 atcaatcccg ccgcctattc gttcacgtcg atcgcgattc tcgacgcgct gaaaatgttc
     7921 ccgggcccga tcatcgaggt gcacatctcc aacatccatc ggcgcgagcc gcactaccac
     7981 cgctcgtacg tgtcgctggc ggcgacggcc gtgatcgccg gcctcgggcc ggccggttat
     8041 cggctcgcgg tgcgcgcgct gcgcgagatg gcgtagccct cgccgcccgt aggcgccacg
     8101 ctcacgccgg tatcgccgcg cctccgacag aaacaggctg cagcgtgccc tatgctgggc
     8161 ctgtttccga ctttcgatgg aggcatcatg gcggacaacc tgaacgaacg cggagcgggc
     8221 gaccgtactc gcatcaacgt cagccagccg cacgagcttt cgtactggtc gcaaaagctg
     8281 ggcgtcaccg aggagcagct gcgaacggcg gtgcagaagg cagggccgat ggccgacgac
     8341 gtcaggcgcc acctcggcaa ggccgcgtga catgccgtac aaggcgattg ccgacctgcc
     8401 ggaggcgcag accgaccagt acaacgagca tcagaagcgt gcgttcctcg aggcgttcaa
     8461 caacgcgtac gacgaatacc acggcgacga gcatcgggcc tttgccgtcg cgcatgcagc
     8521 agccaagcgc gcgggcgagc ggccgggccc cggctgaacg cggagcctac acggcaacgt
     8581 cggcgttgcg ccgcgcccag aacgtgacgc gccgctcgat cagcgcgaag accgcataca
     8641 tcgcgacgcc gagcaccgcc agcaggaaca cgccggcgaa aaccagcggc acgtcgaacg
     8701 tcgagctcgc gaggatcatc atgctgccga tcccgatgtt ggccgccacc gtctcggcga
     8761 ccacggtgcc gacgaacgca agcgaaacgg cgatcttgag cgacgcgaag aaataaggca
     8821 tcgagcgggg caggccgacg cggcgcagga tgtcgagctt gcttgcgccg agcgagcgca
     8881 gcacgtcctc gagctcgggc tcggtcgtgg cgaggcccgt tgcgacattc acgacgaccg
     8941 gaaagaggca catgatggca gccgtcagga tcgcgggaat ggtgccgatg ccgaaccaga
     9001 tcacgaggat cggcacgacg gcgatcttcg gcacgctgta gaagccgatc agcagcggat
     9061 acgtcacgcg atagacgaag cgcgacagtc cgatgcatgc gccgatcgcc gcccccacca
     9121 gcacgccgag cgcgaagccg acgatcgtcg tgtacagcgt ttgcacggcg ttgggcagga
     9181 tggccggcca cttcgtgatc agcacgccga gaaactcgct tggcttcggg agaatgaaca
     9241 ccggcacgct gaacacccgg cagacgatct cccacaccac gaagaacaga atgatcgcca
     9301 gcaccgggaa gacgtggtcg ggagcggcaa gctcacgcac gcgcttgccg atgcgctccc
     9361 gcaggcgccg gggccgcgac gacgcgggtt gcgtgaccgc cgtatccgcg ggtacaccaa
     9421 gctcgtgaac ggatcgcatt cggagtttct ctagacggga cgatcgtgcg cgatgtggcg
     9481 gcgcagctcg tgcacgagtt gcgacgcctg gtcggtgaaa agatcgtcga gcgtgcgcgg
     9541 ccggcggatg tccacgcgat gcgtcgtcag cacgcggccc ggacgcgcgc tcatcacgtg
     9601 gacggtgtcg gcgagaaaca ccgcctcgcg cagatcgtgc gtgacgagga tcaccgtcgg
     9661 gttgcgctcc atccagagct tctgcagcac ctgccacaga tcctcgcgcg tgaacgcatc
     9721 gagcgccccg aacggctcgt cgagcagcag caggcgcgga tcgtggatca gcgcgcggca
     9781 cagcgacgcg cgctgcagca tcccgcccga cagctcccac ggatacttgt cggcgaaatt
     9841 gccgagcccc acttcgccaa gaagcgactg cgccatcgcg cgatacgcgt ccttcttcgc
     9901 gcggaactgg ctgcggtgcg gctcgacgat c
//
LOCUS       DASSVX010000098        12942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_519907, whole genome shotgun sequence.
ACCESSION   DASSVX010000098 DASSVX010000000
VERSION     DASSVX010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12942
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519907"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..575)
                     /gene="apaG"
                     /locus_tag="VFO53_04480"
     CDS             complement(192..575)
                     /gene="apaG"
                     /locus_tag="VFO53_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HET8875564.1"
                     /translation="MAEHKRYEITVAPKVTFLPDQSDPDKNHFVFAYTITVTNTGDVT
                     AQLVSRHWIITDAEHRVQEVKGQGVVGKQPVLRPGESFEYTSGASIPTAVGTMRGSYH
                     MVAEDGLAFDVPIAPFTLSIPRTLH"
     gene            615..1298
                     /gene="rpe"
                     /locus_tag="VFO53_04485"
     CDS             615..1298
                     /gene="rpe"
                     /locus_tag="VFO53_04485"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET8875565.1"
                     /translation="MPHTHRIAPSLLSADFARLGDEVRAVVEAGADMVHLDVMDNHYV
                     PNLTVGPLVCAAIMPHAPVPIDVHLMVKPVDRIVPDFADAGASYMSFHPEASEHVDRT
                     IDLIHDHGCKAGLVFNPATPLDWLMHTLDKLDLVLIMSVNPGFGGQSFIAHALDKLKA
                     ARKLVDAAMARTGREILLEVDGGVKVENIGAIAAAGADTFVAGSAIFGSDDYRATIAA
                     MRGELARVK"
     gene            1434..2915
                     /gene="trpE"
                     /locus_tag="VFO53_04490"
     CDS             1434..2915
                     /gene="trpE"
                     /locus_tag="VFO53_04490"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET8875566.1"
                     /translation="MITEAEFRALAAQGYNRIPLVLESFADLDTPLSLYLKLAQQRYT
                     FLLESVVGGERFGRYSFIGLPAKVRIRVKGTHVEVEDARGVIERHDGDPLAFLRAFLG
                     RFRAAPLPGLPRFCGGLAGYFGYDTVRHIEPTLGVRKPDVDDGLDDLPDMLLLLTDEL
                     AVIDNLGGKISLIVYADPAEADAYARAQERLQHLRRASRAPLAIPFQTATEVTRAESE
                     TGADAFQAAVRRAKDYIAAGDIMQVVLSQRLHLPFAASPLSLYRALRSLNPSPYMFYY
                     DFGDFHVVGASPEILVRKEASTITLRPIAGTRPRGDTRDADERLARELAADPKEIAEH
                     VMLLDLGRNDVGHVAATGSVRVTEQMAIERYSHVMHIVSNVEGNLPPGTTSFDVLAAS
                     FPAGTVSGAPKVRAMQIIDELEPSARGVYAGAVGYISFQDDMDLAIAIRTAVLRNGML
                     YVQVGAGIVHDSVPEAEWQETFNKARAVLRAAELVQLGLDAES"
     gene            3093..3545
                     /locus_tag="VFO53_04495"
     CDS             3093..3545
                     /locus_tag="VFO53_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875567.1"
                     /translation="MDKMQLRAAARAFIRAAQMPGIDEPPLRTTREQAKATIDDTPAR
                     TRGRTPPPLDKARARQACAPMGALHSIVVTVDVRKRRRTWYIVDPDPAVAPQYSAAGL
                     DLTPGCKVLVDAEAMRPVALVDFDPAVSASDSSPMPHGFNPYFDLPAG"
     gene            3565..4458
                     /locus_tag="VFO53_04500"
     CDS             3565..4458
                     /locus_tag="VFO53_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET8875568.1"
                     /translation="MRRPVESAGSLIRLDYTVNLHYDVFGDADFVFSVHAAHTGQQRV
                     LREALVVAPSVAWRVDTEPAFGNRLLRLRASAGPLDVRYDASVEVAHYLREPGQVIAG
                     ALADLPVEVLPFLRPSRYCQSDEVQQLAWREFGALAPGHEQVDAVCRWVRSRVEFRIG
                     TSTIATSALDTLEHGVGVCRDFVHATIALLRGLNYPARFVTGVDYGAPAALGPSDFHS
                     YVEAWIGGRWWIFDPTGITPLTGLIRIGTGRDAVDVAFATMFGNVRGGMPKVAFAALD
                     DPDAGVGLPQPTTLAVSTAAL"
     gene            4645..5244
                     /locus_tag="VFO53_04505"
     CDS             4645..5244
                     /locus_tag="VFO53_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875569.1"
                     /translation="MGTQQSRSGHGRQGSQQHSQQSERRPEQDRDLQRNKQSDRSQQQ
                     HPRSDGGHQQQQGSSSSGQQPAGSGGQPQMQPERQQSQLGGNRDFTHKMENEGREQNR
                     EQQSMRRQQSQQDVQGEGNYTASKQYNDATKDYARSGRVDAAARAAAPKSSADARAMD
                     AAEAEGKRHAKGEDPALNRRASSSSGESSETPRPGHEEQ"
     gene            5248..5442
                     /locus_tag="VFO53_04510"
     CDS             5248..5442
                     /locus_tag="VFO53_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875570.1"
                     /translation="MRPYLHDPVASQLNSIPLLDASSLPSNDDWALLESINAYAEAAT
                     DFEPDHDRYGRVRVSAFYDE"
     gene            5511..5678
                     /locus_tag="VFO53_04515"
     CDS             5511..5678
                     /locus_tag="VFO53_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HET8875571.1"
                     /translation="MLQYAAVFFVIALIAAVFGFTGIAVGAAEIAKVLFFIFLALFVI
                     SLLVGMFRRSP"
     gene            5851..6438
                     /locus_tag="VFO53_04520"
     CDS             5851..6438
                     /locus_tag="VFO53_04520"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET8875572.1"
                     /translation="MLLMLDNYDSFTWNLVQYLGELGAAVKVVRNDAASLDEIESMHP
                     EGIVISPGPCTPSEAGISVPLVRRFAGRVPILGVCLGHQAIGQAFGARVIRAQRVMHG
                     KLSSIVHDQRGVFAGVPSPFNATRYHSLALERASLPEVLDVTATADDGEIMAIRHREL
                     PVEGVQFHPEAILTEHGRKVLENFLEGVGTATAVA"
     gene            6485..7522
                     /gene="trpD"
                     /locus_tag="VFO53_04525"
     CDS             6485..7522
                     /gene="trpD"
                     /locus_tag="VFO53_04525"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_035822072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET8875573.1"
                     /translation="MAITPQDALQRTIEHREIFHDEMLSLMRRIMSGEVSPVMIAALT
                     VGLRVKKETIGEIAAAAQVMREFATRVDVEGAEGLLDIVGTGGDASHTFNISTASMFV
                     AAAAGARVAKHGNRSVSSRSGSADVLEALGANIALSPAQVARCIAEAGVGFMFAPAHH
                     GAMKHAAAVRKELGVRTIFNILGPLTNPAGAGHSMVGVFHPDLVGIHVRVLQRLGSRR
                     ALVVYGKDGMDEVSLGAATMVGELKDGEVTEYEIHPEDFGLHMKSNRGLKVTDARQSK
                     EMVLEALGDADGTPREIVVLNAGTALYAAGIADSIGDGIARAREVIASGAARRKLDAF
                     VAATQAIGKEA"
     gene            7519..8364
                     /gene="trpC"
                     /locus_tag="VFO53_04530"
     CDS             7519..8364
                     /gene="trpC"
                     /locus_tag="VFO53_04530"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET8875574.1"
                     /translation="MNSDLSANGRQAAAGPAADSDILAKILTTKAEEVIAAQVARPFA
                     IVDREARAAASPRGFADAIRARIGSGRAAVIAEIKRASPSKGVLRAEFDPASIAASYE
                     RAGATCLSVLTDRPFFQGAPEYLVEARAACALPALRKEFIVDEYQVAESRALGADAVL
                     LIVAALDDARLAGLEACARDYAMDVLVEVHDAAELDRALALETPLIGINNRNLRTFHV
                     SLQTTIDLLPRIPPERIVVTESGILARRDVAQMRRHGVDAFLVGEAFMRAPDPGAALT
                     ALFDA"
     gene            8406..9497
                     /locus_tag="VFO53_04535"
     CDS             8406..9497
                     /locus_tag="VFO53_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875575.1"
                     /translation="MSAIAADGVSKVWTTASGAVRAVDGVSFAFDAGTLNVLLGPSGC
                     GKSTTLRLVAGLEVCDGGRILIDHRDVTRLPPAARSIAMVFQSYALFPHLTVAENVLF
                     GLRVRRTPAAERSERLARVADLLGLTPLLERKPSQLSGGQQQRVALGRAIIAQAPVCL
                     MDEPLSNLDAQLRAEMRLEIRALQRKLGITIVYVTHDQVEAMSMADRVVLMKNGRIEQ
                     NGTPVQLYETPANTFAARFIGTPPMNLLRLEAGAGGAVIAGTEGPALVDARFAQGLLG
                     VRPEHVELAFERGIRGGVDSVEYLGGDSLVSCRVGGQPLAARVAGSVALARGDAVWLR
                     WAPGAQHYFDAGGTRAASEPQESPATLMA"
     gene            9530..10819
                     /locus_tag="VFO53_04540"
     CDS             9530..10819
                     /locus_tag="VFO53_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8875576.1"
                     /translation="MKLGRMLRAFAGAVLALVAAAAIAQSPVEVPFFYPVAVGGPITK
                     IIDAFAADFEKENPGIKLRPIYSGSYQESIAKALTAVKSGDPPVTSILLSTDMFTLID
                     EDAIVPFDDLIKTADDKAWLKSFYPAFMENSQTGGKTWGIPFQRSTVVLYYNKEAFKE
                     AGLDPNRPPATWTEMREYAQKLTKRDASGNVSQWGVQIPSSGFPYWLFQGLAIENGVN
                     LMNQAGTEVYYDKPEVVAALSYWLDLVNKYKVHPPGIVEWGTTPKDFFERKVAMMWTT
                     TGNLTNVKANAKFDFGVAMLPASKQRGSPTGGGNFYIFKKSTPAQREAAFKFIKWVTT
                     PERAAQWGIDTGYVAVRADAWETPAMKKYVAGFPAAAVARDQLPFAKAELSTHDNQRV
                     TKALNDGLQAALTGTKSAAQALKDAQREGDRLLRSYK"
     gene            10927..11802
                     /locus_tag="VFO53_04545"
     CDS             10927..11802
                     /locus_tag="VFO53_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8875577.1"
                     /translation="MTRTREWLYAWLLLLPAATLLALFTHYPAIANLWHSFYSTPKGA
                     RPARFIGLDNYRALVDDPIFWQALSNNLWYALGTIPLSISLALLMAVWVNGRLPGRGF
                     LRLAYFTPTVLPMIAVANIWLFFYTPGYGLLEQITGFFGWPSHNWLGSQSTALPALMV
                     VTIWKEAGFFMIFYLAALQSMSPHLAEAAAIEGASRSYFFRRITLPLLMPTTLFVLVN
                     AVINAFRLVDHIVVMTRGGPDNATALLLYYIFEIGFRFWDSSYAAALTMVLLVLLGAV
                     AIVQFAFLERRTFYQ"
     gene            11828..12628
                     /locus_tag="VFO53_04550"
     CDS             11828..12628
                     /locus_tag="VFO53_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET8875578.1"
                     /translation="MSHSLETLGAWVLGVLWVLPLVYAFWTAFHPPAYSTNFTLSAPL
                     TLGNFVKAWQAAPFARYFANTVILVTMVLAAQLVLVTLAAYAFARFRFPGRDVAFALV
                     LVQLMVMPDVLIVENYRTMTALGLKDTILSIGLPYMASAFGIFLLRQTFKTVPRELDE
                     AARVEGCSPLATLWKVYVPLARPTYIAYALVSVSYHWNNFLWPLIITNSVESRPVTVG
                     LQVFSATDQGIDWSIISAATLLTTGPLLIAFLLFQRQFVQSFMRAGIR"
     gene            12713..>12942
                     /locus_tag="VFO53_04555"
     CDS             12713..>12942
                     /locus_tag="VFO53_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HET8875579.1"
                     /translation="MKLITWNVQWCRGVDLVVDPARIVREAQRLDDFDVLCLQEIADN
                     YPDPRLPGSSGEDQFARLAQLLPQYTAIPGIAV"
BASE COUNT         2105 a         4323 c         4316 g         2198 t
ORIGIN      
        1 gcacgcagcg catcagtatc ggggttgcat gtcgatgggg gaggcgtggc aatggcgtgg
       61 cttgtattcg aatcgttcgt cgcattgctg gttctggtcg ggatcgtcgc gtggacgatg
      121 gcgccgagcc gcaagcgcga acggcggccg gacgacgagg attgagccgc gccggcacgg
      181 cgcacgcgga gtcaatgcag cgtgcgcggt atcgacagcg tgaacggcgc aatggggacg
      241 tcgaacgcga ggccgtcctc cgctaccatg tgatacgacc cgcgcatcgt gccgaccgcg
      301 gtgggaatgc ttgcgccgct cgtgtattcg aagctttcgc cgggccgcag caccggctgc
      361 ttgccgacca ccccctgtcc cttcacttcc tggacgcggt gctcggcatc ggtgatgatc
      421 cagtggcggc tgacgagttg cgcggtgacg tcgcccgtgt tggtgacggt gatcgtgtac
      481 gcgaacacga aatggttctt gtccggatcg gattgatccg gaagaaaggt cactttcggc
      541 gctacggtga tttcgtaacg tttgtgctcc gccatgtcgc tgctgcccga tcttttctga
      601 gattatagtc cccgatgccg cacactcacc gcattgcacc gtcgttgctg tctgccgatt
      661 tcgcacgtct cggcgacgaa gtgcgtgcgg tcgtcgaggc cggcgccgac atggttcacc
      721 tggatgtgat ggacaaccat tacgtgccca atctgacggt gggtccgctc gtatgcgccg
      781 ccatcatgcc gcacgcgccg gtgcccatcg acgtgcacct gatggtgaag ccggtcgacc
      841 gcatcgtgcc cgatttcgcc gatgcgggcg cctcgtacat gagctttcac cccgaagcct
      901 ccgagcatgt cgaccgcacg atcgacctga ttcacgacca tggctgcaag gcggggctcg
      961 tgttcaatcc cgccacgccg ctcgactggc tgatgcacac gctcgacaag ctggacctcg
     1021 tgctcatcat gtcggtcaat ccgggttttg gcggccagtc gttcattgcg catgcgctcg
     1081 acaagctgaa ggccgcgcgc aagctcgtcg acgccgcgat ggcgcgcacg ggacgcgaaa
     1141 tcctgctcga ggtggatgga ggcgtcaagg tggagaacat cggcgccatc gcagccgccg
     1201 gcgccgacac gttcgtcgcc ggcagcgcga tcttcggcag cgacgattac cgggcaacga
     1261 tcgcggcaat gcgcggcgag ctcgcccgag tgaaatgaca tgagcggcgc cgggccgttc
     1321 ccaagccgcg aattgcgccc cctttggggg cagcgcagcg gcgtgagccg caagcgtggg
     1381 gggaacatca gcggcgccgg gccgttccca agccgcgaat tgcgtggggg tccatgatta
     1441 cggaggcgga gtttcgcgcg ctcgctgcgc agggctacaa ccggatcccg ctggtgctcg
     1501 agtcgttcgc cgatctcgac acgccgctgt cgctgtacct gaagcttgcg cagcagcgct
     1561 ataccttcct gctcgaatcg gtcgtcggcg gcgagcgctt cgggcgctac tcgttcatcg
     1621 gcctgccggc gaaggtgcgc atccgcgtca agggcacgca cgtggaggtc gaggacgcgc
     1681 gcggcgtcat cgagcgccac gacggcgacc cgctcgcgtt cctgcgtgcg ttcctcggcc
     1741 gctttcgcgc ggcgccgcta cctggcctgc cgcgcttctg cggcggtctg gccggctatt
     1801 tcggctacga caccgtgcgt cacatcgagc cgacgctcgg cgtgcgcaaa cccgacgtcg
     1861 acgacgggct cgacgatctg cccgacatgc tgctgctgct caccgacgag ctcgccgtca
     1921 tcgacaacct cggcggcaag atttcgctga tcgtctacgc cgatcccgcc gaagccgacg
     1981 cgtatgcacg cgcgcaggaa cgcctgcaac acttgcgccg cgcctcgcgt gcgccactcg
     2041 ccattccgtt ccagacggcg acggaagtca cgcgcgccga aagtgaaacg ggcgccgacg
     2101 cgttccaggc ggcggtgcgg cgcgccaagg actacatcgc ggccggcgac atcatgcagg
     2161 tcgtgctgtc gcagcggttg catcttccgt tcgcggcatc accgttgtcg ctctaccgtg
     2221 cgctgcgctc gctcaatcca tcgccgtaca tgttctatta cgacttcggc gacttccacg
     2281 tcgtcggcgc ctcgcccgaa atcctcgtgc gcaaggaagc gtcgacgatc acgctgcggc
     2341 ccatcgccgg cacgcgtccg cgcggcgaca cgcgcgacgc cgacgagcgg ctcgcccgcg
     2401 agctcgccgc cgatccgaag gagatcgccg agcacgtcat gctgctcgac ctcggccgca
     2461 acgacgtcgg ccacgtcgcc gccaccggca gcgtgcgggt gaccgagcag atggcgatcg
     2521 agcgctattc gcacgtgatg cacatcgtgt cgaacgtcga aggcaatctg ccgccgggca
     2581 cgacgagctt cgacgtgctg gcggcgagct ttccggcggg gacggtgagc ggcgcgccca
     2641 aggtgcgggc gatgcagatc atcgacgagc tcgagccgtc cgcgcgcggc gtctacgcgg
     2701 gcgccgtcgg ctacatcagt ttccaggacg acatggatct cgccatcgcg attcgcacgg
     2761 cggtgctgcg aaacggcatg ctgtacgtgc aggtcggcgc cggtatcgtg cacgattcgg
     2821 tgcccgaagc agaatggcag gaaacgttca acaaggcgcg cgcggtgctg cgcgcggccg
     2881 agctcgtgca gttgggcctc gacgcggagt cgtagcggat ttaccaacgg cgtcgcgcgc
     2941 cgtcggcgca ggttttaccg gcgcaggtaa ttcctgccgg gaagcgcatc gtcgcagtct
     3001 cgacgtgccg ttgatttggc acgagtttcg accgcgaggg aactggcatg ccgggtgctg
     3061 gctcacaagg gtcacagatc gagagatcct ccatggacaa aatgcagttg cgtgccgccg
     3121 cgcgcgcgtt cattcgcgct gcgcaaatgc caggcatcga cgagcccccg ctgcgcacca
     3181 cgcgcgagca ggcgaaggca acgatcgacg acacgccggc gcgaacgcgc ggccgcacgc
     3241 cgccgccgct cgacaaggca cgcgcacgcc aggcgtgcgc gcccatgggc gcgctgcatt
     3301 cgatcgtggt caccgtcgac gtgcgcaaac gccggcgcac ctggtacatc gtcgatcccg
     3361 atccggccgt cgcaccgcag tattcggccg cggggctcga tctgacccct ggctgcaagg
     3421 tgctcgtcga cgccgaagcg atgcgtccgg tcgcgctcgt cgatttcgat cccgccgtga
     3481 gcgcgagcga ctcgtcgccg atgccgcatg gcttcaatcc ctatttcgat ctgcccgccg
     3541 gctgagcggc ggtggctatt gcacatgcgt cgcccggtcg aatcggccgg tagcctgatt
     3601 cgcctcgact acacggtcaa cctgcactac gacgtctttg gcgacgccga tttcgtcttc
     3661 agcgttcacg cggcacacac cgggcagcag cgcgtgctgc gcgaggcgct cgtcgtcgcg
     3721 ccctccgttg cctggcgcgt cgacaccgag ccggcgttcg gcaatcggct gttgcgcctg
     3781 cgcgcatccg ccggcccgct cgacgttcgc tacgacgcca gcgtcgaggt ggcgcattac
     3841 ctgcgcgagc cgggtcaggt aatcgcgggc gccctcgcgg accttccggt cgaagtactg
     3901 ccgttcctgc gcccgagccg ctattgccag tccgacgagg tgcagcagct cgcctggcgc
     3961 gagttcggcg cgctcgcgcc gggccacgag caggtcgacg ccgtttgccg ctgggtccgc
     4021 tcgcgcgtcg aattccgcat cggcacctcg accatcgcca cctcggcgct cgatacgctc
     4081 gagcacggcg tgggcgtgtg ccgcgatttc gtgcatgcga cgatcgcact gttgcgcggg
     4141 ctcaactatc cggcgcgatt cgtgaccggc gtggattacg gcgcacccgc cgcactcggg
     4201 ccatccgact tccattcgta tgtcgaagcg tggatcgggg gacgctggtg gattttcgac
     4261 cctacgggga tcacgccgct caccggattg atccgtatcg gcaccggacg cgatgccgtc
     4321 gacgtggcgt ttgccacgat gttcggcaac gtgcgcggcg gcatgccgaa agtcgcgttc
     4381 gcagcactcg acgatcccga cgccggcgtc gggctgccgc agccgacgac gctcgccgtc
     4441 tcaaccgcgg cactttgacg caagcaccgc gctcgcttgc gccgtccggc gtaggactga
     4501 cggggacatc cgcctgccgg cgcaccgact tcccgaatcg gcgctgctcc gactgcgcct
     4561 gcacgagcgc caccgcagaa tgcctccaca tcgacgccgc cgggtgaaac ggcccgggcg
     4621 cctgcgtcat aacgaggagg cttcatgggt acacagcaaa gccgatcggg gcatggccgc
     4681 caaggcagcc agcagcacag ccagcagtcg gagcgccggc cggaacagga tcgcgacctg
     4741 cagcgcaaca aacaaagcga tcgcagccag cagcagcatc cgcgcagcga tggcggtcac
     4801 cagcaacagc agggctcgag ttccagtggc cagcagccgg caggatcggg cgggcagccg
     4861 cagatgcagc cggagcgcca gcagagccag ctcggcggca atcgcgactt cacgcacaag
     4921 atggaaaacg aaggccgcga gcagaaccgc gaacagcaga gcatgcgccg ccagcaaagc
     4981 cagcaggacg tgcaaggcga gggcaattac accgcgtcga agcaatacaa cgatgcgacg
     5041 aaggattacg cgcggtcggg tcgcgtcgac gccgcggcac gcgccgccgc gccgaaatcg
     5101 agcgccgacg cgcgggcaat ggatgcggcc gaggccgagg gcaagcggca cgcgaagggc
     5161 gaggatcccg cgctgaaccg tcgcgcatcc tcatcgtccg gggagtcgtc cgaaacgccg
     5221 cgaccgggcc acgaggagca atgagcgatg cgcccctatc tgcatgatcc ggtggcgtcg
     5281 cagctcaatt cgattccgct gctcgatgcg agctcgctgc cgtcgaacga cgactgggca
     5341 ctgctcgaat cgatcaatgc gtacgccgaa gcggcgaccg acttcgagcc ggaccacgac
     5401 cgttacggcc gcgtccgggt gagcgccttt tacgacgagt aggaccgcga gggtccgcat
     5461 gcgcggatgg tcgcggaatt tgttggtcca atcagttcgg gaggtttgtc atgctgcaat
     5521 acgctgcggt gtttttcgtg atcgcgttga tcgcggcggt attcggattc accggcatcg
     5581 cggtcggtgc tgccgaaatc gccaaggtcc tcttcttcat cttcctcgcg ctgttcgtca
     5641 tttcgctgct cgtagggatg ttccggcgct cgccgtgatg cccggcgcca atcgcatcgc
     5701 gcgacattag aacgacgcag taccggcagg accggttacc tcgaagcccc tcgaacggcg
     5761 agttcggggg gtcttttttt ggtcgagcta gaatgcggtg agattgcatc gccgcgggtc
     5821 gcaccgcgcg gcgagcaaag gattgccgcc atgctcctga tgctcgacaa ctacgacagc
     5881 ttcacctgga acctcgtcca gtacctgggc gagctcggcg ccgcggtcaa ggtcgtgcgc
     5941 aacgacgccg cctcgctcga cgaaatcgag tcgatgcacc ccgaggggat cgtcatctcg
     6001 cccggaccgt gcacgccgag cgaggccggg atctcggtgc cgctcgtgcg gcgcttcgcg
     6061 ggcagggtgc cgatcctcgg cgtgtgcctc ggtcaccagg cgatcgggca ggcgttcggc
     6121 gcgcgcgtca tccgggcgca gcgcgtgatg cacggcaagc tgtcgtcgat cgtccacgat
     6181 cagcgcggcg tgttcgccgg cgtcccgtcg ccgttcaacg ccacgcgcta tcactcgctc
     6241 gcgctcgagc gcgccagcct gcccgaggtg ctcgacgtca cggcgacggc ggacgatggc
     6301 gaaatcatgg cgatacgtca ccgcgagctg ccggtcgaag gcgtgcagtt tcaccccgag
     6361 gcaatcctca ccgagcacgg caggaaggtg ctcgagaact tcctcgaggg cgtcggcact
     6421 gcgactgccg tggcatgaga gcgaccggga cgctttcgct gccgaacctg ctgtcctgac
     6481 tcccatggcc atcacgcccc aggatgcgtt gcagcgcacg atcgagcacc gcgagatctt
     6541 ccacgacgag atgctgtcgc tgatgcggcg gatcatgagc ggtgaagtct cgccggtgat
     6601 gatcgccgca ctcaccgtcg ggctccgcgt caagaaggaa accatcggcg aaatcgccgc
     6661 cgcggcgcag gtgatgcgcg agttcgcgac gcgtgtcgat gtcgaaggcg ccgagggcct
     6721 gctcgacatc gtcggcacgg gcggcgacgc gtcgcacacg ttcaacatct cgacggcgtc
     6781 gatgttcgtg gcggcggccg cgggcgcacg cgtggcgaag cacggcaacc ggtcggtgtc
     6841 gtccaggtcc ggcagcgccg acgttctcga ggcgctcggc gcgaacatcg cgctttcgcc
     6901 ggcgcaggtg gcgcgctgca tcgcggaggc cggcgtcggc ttcatgttcg cgcccgccca
     6961 ccacggcgcg atgaagcacg cggcggcggt gcgcaaggag ctcggcgtgc gcacgatctt
     7021 caacatcctc gggccgctga ccaaccccgc gggtgcgggc cacagcatgg tcggcgtctt
     7081 ccaccccgat ctcgtcggca ttcacgtccg cgtgctgcaa cggctcggca gccggcgtgc
     7141 cctggtggtt tacggcaagg acggcatgga cgaagtatcg cttggcgcgg cgacgatggt
     7201 cggcgagctc aaggacggcg aagtcaccga gtacgagatt catcccgagg acttcggcct
     7261 gcacatgaag tcgaaccgcg gcctcaaggt caccgacgcc cggcaatcga aggaaatggt
     7321 cctcgaggcg ctcggcgatg ccgacggcac gccgcgcgag atcgtcgtgc tgaacgccgg
     7381 gaccgcactt tatgcggcgg gcatcgccga ttcgatcggg gacggcattg ctcgtgcgcg
     7441 cgaggtgatc gccagtggcg cggcacgacg caagctcgac gcgttcgtcg ccgcaacgca
     7501 ggcgatcggc aaggaagcat gaattcagac ctgagcgcca acggtcggca agcagcggcg
     7561 ggtcccgcag ccgacagcga catcctcgcg aagattctga cgaccaaggc cgaggaggtg
     7621 atcgcggcgc aggtcgcacg tccgttcgcc atcgtcgatc gcgaggcgcg ggctgccgcg
     7681 tcgccgcgtg gctttgcgga cgcgatccgt gcgcggatcg gcagtggacg cgccgccgtc
     7741 atcgcggaaa tcaagcgcgc gagcccgagc aagggcgtgc tgcgcgccga gttcgatccc
     7801 gcgtcgatcg ccgcgtccta cgagcgcgcc ggtgcaacgt gcctctcggt gctgaccgac
     7861 cggccgttct tccagggcgc gcccgaatac ctggtcgagg cgcgcgcggc atgcgcgctt
     7921 cccgcgttgc gcaaggaatt catcgtcgac gaataccagg tcgccgagtc gcgcgcgctc
     7981 ggcgccgatg ccgtgctgct catcgtcgcg gcgctcgacg acgcgcgact cgccgggctc
     8041 gaggcgtgtg cgcgcgatta cgcaatggat gtgctcgtcg aagtgcacga tgcggccgag
     8101 ctcgatcgtg cgctggcgct cgaaacgccg ctgatcggca tcaacaaccg caacctgcgc
     8161 actttccacg tttcgctgca gacgacgatc gatctgctcc cgcgcatccc gccggaacgc
     8221 atcgtcgtca ccgaaagcgg catcctcgct cggcgcgacg tcgcgcagat gcgcaggcat
     8281 ggcgtcgacg ccttcctcgt cggcgaggca ttcatgcgcg cgcccgatcc aggcgccgcc
     8341 ttgacggcgc tgttcgacgc ctgatgcatt gcgcatgaac gcgcatcgtt ggccgttccc
     8401 gcaccatgtc ggcgattgcc gctgacggcg tcagcaaggt ctggacgacc gcgagtggcg
     8461 cggtgcgcgc cgtcgacggc gtctcgttcg cattcgacgc gggcacgctc aacgtgctgc
     8521 tcgggccgtc gggctgcggc aagtccacca cgctgcgcct cgtcgccggc ctcgaggtct
     8581 gcgacggcgg gcgcatcctg atcgatcacc gcgacgtgac gcggcttccg ccggccgcgc
     8641 gctcgatcgc gatggtgttc cagagctacg cgctgttccc gcaccttacg gtggcggaga
     8701 acgtgctgtt cggattgcgc gtgcgccgca cgccggcggc cgagcgaagc gagcgcctcg
     8761 cgcgcgttgc cgatctgctc gggctgacgc cgctcctcga gcgcaagccg tcgcagcttt
     8821 cgggcgggca gcagcagcgc gtggcgctcg gccgcgcgat catcgcgcag gcgcccgtgt
     8881 gcctgatgga cgagccgttg tcgaatctcg acgcgcagct tcgcgccgag atgcggctcg
     8941 aaatccgtgc gctgcaaaga aagctcggca tcacgatcgt ctacgtgacg cacgaccagg
     9001 tcgaggcgat gtcgatggcc gaccgcgtcg tgctgatgaa gaacggcagg atcgagcaga
     9061 acggaacgcc ggtgcagctc tacgagacgc ccgcaaacac gttcgctgcg cgcttcatcg
     9121 gcacgccgcc gatgaacctg ctgcgcctcg aggcgggggc gggcggcgcg gtgatcgcgg
     9181 gcaccgaagg tccggcgctc gtcgatgcgc gcttcgcgca gggattgctc ggcgtgcgtc
     9241 ccgagcacgt cgagctcgcg ttcgagcgcg gcattcgcgg cggcgtcgac agcgtcgaat
     9301 atctcggcgg cgattcgctc gtcagttgcc gcgtgggcgg gcagccgctt gcggcgcgcg
     9361 tcgccggcag cgtcgcgctt gcgcgcggcg atgccgtgtg gctgcgctgg gcgcccggcg
     9421 cgcagcacta tttcgacgcg ggcggtacgc gcgcggcgag cgagccgcag gaatcgccgg
     9481 ccacgttgat ggcctgatgc accatttcgt ccaacaaagg ggagaagcca tgaagcttgg
     9541 tcgcatgttg agggcattcg cgggcgcggt gctcgcgctg gtcgccgccg ccgcgatcgc
     9601 gcagtcgccg gtcgaggtgc cgttcttcta tccggtcgcg gtcggcggac cgatcacgaa
     9661 gatcatcgac gcattcgcgg ccgatttcga aaaggaaaat cccgggatca agctgcggcc
     9721 gatctattcg ggcagctacc aggaatcgat cgccaaggcg ctgaccgcgg tgaaaagcgg
     9781 cgatccgccg gtgacgtcga tcctgctgtc gaccgacatg ttcacgctga tcgacgagga
     9841 cgcgatcgtg ccgttcgacg atctcatcaa gacggccgac gacaaggcgt ggttgaaaag
     9901 cttctatccg gcgttcatgg agaacagcca gacgggcggc aagacgtggg gcatcccgtt
     9961 ccagcgctcg acggtcgtgc tctattacaa caaggaggcg ttcaaggagg cgggactcga
    10021 tccgaaccgg ccgccggcaa cgtggaccga gatgcgcgaa tacgcgcaga agctcacgaa
    10081 gcgagatgcg tcgggcaacg tctcgcagtg gggcgtgcag attccgtcgt cgggctttcc
    10141 gtactggctg ttccagggcc tcgcgatcga gaacggcgtc aacctgatga accaggcggg
    10201 caccgaggtc tattacgaca agccggaagt cgtcgccgcg ctttcgtact ggctcgacct
    10261 cgtcaacaag tacaaggtgc atccgccggg catcgtcgaa tggggcacga caccgaagga
    10321 cttcttcgag cgcaaggtcg cgatgatgtg gacgaccacc ggcaatctca cgaacgtgaa
    10381 ggcgaatgcc aagttcgact ttggcgtcgc gatgctgccg gcctccaagc agcgcggcag
    10441 cccgaccggc ggtggcaact tctatatctt caagaaatcg acgcccgcgc agcgcgaagc
    10501 ggcgttcaag ttcatcaagt gggtgacgac gcccgagcgc gccgcgcaat ggggcatcga
    10561 caccggttac gtcgccgtgc gtgccgacgc atgggagacg ccggcgatga aaaagtacgt
    10621 cgccggtttc cccgccgcgg cggtcgcgcg cgatcagctc ccgttcgcga aggccgagct
    10681 gtcgacgcac gacaatcagc gggtgacgaa ggcgttgaac gacggcctgc aggcggcgct
    10741 caccggcacg aagagtgccg cgcaggcgtt gaaggatgcg cagcgcgaag gggaccggct
    10801 gctgcgcagc tacaagtagc gcgtgacgca gaacgagctc gtgcatacga cgtccggtcg
    10861 attcgacaac ctcggcagtt ggctcagcct ccctctcccc gcaggcgggg agagggtgaa
    10921 tgcagcatga cgcgaacgcg cgagtggctt tacgcctggc tgctgctgct gccggccgcg
    10981 acgctgctcg cgctgttcac gcactacccg gccattgcca acctgtggca cagcttctac
    11041 tcgacaccga agggcgcgcg tccggcaagg ttcatcgggc tcgacaacta ccgcgcgctc
    11101 gtcgacgacc cgatcttctg gcaggcgctg tcgaacaacc tgtggtacgc gctcggcacg
    11161 attccgctgt cgatctcgct tgcgctgctg atggcggtgt gggtcaacgg gcgcctgccc
    11221 ggacgcggct tcctgcgcct cgcgtatttc acgcccacgg tattgccgat gatcgccgtc
    11281 gccaacatct ggcttttctt ctacacgccg ggctacggtc tgctcgagca gatcaccgga
    11341 tttttcggat ggccgagcca caactggctc ggcagccaga gcaccgcgtt gcccgcgctg
    11401 atggtcgtga cgatctggaa ggaggccggt ttcttcatga tcttctacct ggccgcgttg
    11461 cagtcgatgt cgccgcatct tgccgaggcg gccgcgatcg agggcgcgtc gcgcagctac
    11521 ttctttcgcc gcatcacgct gccgcttttg atgcccacga cgctgttcgt gctggtcaac
    11581 gccgtgatca acgcgttccg cctcgtcgac cacatcgtcg tcatgacgcg cggcggaccc
    11641 gacaatgcca cggcactgct gctgtattac atattcgaga tcggcttccg gttctgggat
    11701 tcgtcgtacg cggcggcgct gacgatggtg ctgctggtcc tgctcggcgc cgtggcgatc
    11761 gtccagttcg cattcctcga gcgccgcacg ttctaccagt gagatgagcg taaacgcccc
    11821 gcgcgcaatc tcgcactcgc tcgaaacgct cggcgcgtgg gtgctcggcg tgctgtgggt
    11881 cctgccgctc gtgtacgcgt tctggaccgc gttccacccg cccgcgtact cgacgaactt
    11941 cacgctgtcg gcccccttga cgctcggcaa tttcgtcaag gcctggcagg cggcaccgtt
    12001 tgcgcgctat ttcgcgaaca cggtgatcct cgtcacgatg gtgctcgccg cgcaactcgt
    12061 gctggtgacg ctcgccgcct acgcattcgc gcgctttcgc ttccccgggc gcgacgtcgc
    12121 gttcgcgctc gtgctcgtgc agctcatggt gatgcccgac gtgctgatcg tcgagaatta
    12181 ccgcacgatg accgcgctcg ggctgaagga cacgatcctg tcgatcggcc tgccgtacat
    12241 ggcgagcgcg ttcggcatct ttctgttgcg ccagacgttc aagaccgtgc cgcgcgagct
    12301 cgacgaggcg gcgcgcgtcg aaggctgctc gccgctcgcg acgctgtgga aggtctacgt
    12361 gccgctcgcg cggccgacgt acatcgcgta cgcgctcgtg tccgtctcgt accactggaa
    12421 caacttcctc tggccgctca tcatcaccaa ttcggtggaa tcgcggccgg tcaccgtcgg
    12481 cctgcaggta ttctcggcca ccgaccaggg catcgactgg tcgatcatca gcgcggcgac
    12541 gctgctcacg accgggccgc tgctgatcgc attcctgctg ttccagcggc aattcgtcca
    12601 gtcgttcatg cgagcgggca tccgctgacg cccgcatgcc gcgacattcc cattcgatcg
    12661 tgccgcttgc gttgccgttt gcgccaaatc gatcatccag aacccgacga ccatgaaact
    12721 gatcacgtgg aacgtgcaat ggtgccgcgg tgtcgacctc gtcgtcgacc cggcgcgcat
    12781 cgtgcgcgag gcgcagcgcc tcgatgattt cgacgtgctg tgcctgcagg agatcgccga
    12841 caactacccg gatccgcggc tgcccggcag cagcggcgag gaccagttcg ctcgtctcgc
    12901 gcaactgttg ccgcagtaca cggcgatccc ggggattgcc gt
//
LOCUS       DASSVX010000099         4464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1503315, whole genome shotgun sequence.
ACCESSION   DASSVX010000099 DASSVX010000000
VERSION     DASSVX010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503315"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFO53_04560"
     CDS             complement(<1..700)
                     /locus_tag="VFO53_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HET8875580.1"
                     /translation="MTEVAAANPSRPSAGACEPQDDATECWPALALADWKDTCDTLHM
                     WTQIVGKLRLATAPLVNHWWNVTLYLNARGLTTSAMPYRGRTLELQFDFVRHELVLET
                     SEGMVRTMPLAPRTVADFYHSFIEMLHGADIDVAIWPMPVEVPDPIAFAQDRTHASYD
                     RDAVQRFWRVLRSVERVFNVFRADFIGKCSPVHFFWGSLDLAVTRFSGRRAPERAGAD
                     AITREAYSHEVSRVG"
     gene            complement(697..1980)
                     /locus_tag="VFO53_04565"
     CDS             complement(697..1980)
                     /locus_tag="VFO53_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET8875581.1"
                     /translation="MSGPTTAPSAPATRLLRKLGVADAVVIGLGSMIGAGIFAALAPA
                     AAAAGPALLVGLMVAAFVAYCNATSSAALAALYPESGGTYVYGRRRLGAFWGFLAGWG
                     FVTGKLASCAAMALTFAAYAAPAFSRPLAILAVIALTAVNYFGISRTVLATRVIVAVV
                     LACLAVVVFAAAFGGNVDVTRAIPGSAPGIRGILQAAGFLFFAFAGYARIATLGEEVI
                     DPARTIPRAIPLALGLTLLVYAAVAISALVTVDARDLAGTRAPLVHVVESGRWSALAP
                     AVRIGAAIASLGVLLSLLAGVSRTAFAMAGNRDLPRFLASVHARHRVPDHAELAAGAI
                     VALIVLIADVRDAIGFSSFAVLVYYAIANASAVTLRRDERRWPRALAIAGLAGCALVA
                     ISLPWQSILEALVLLLAGAAVYAMRRRQRSSGAQR"
     gene            2025..2918
                     /locus_tag="VFO53_04570"
     CDS             2025..2918
                     /locus_tag="VFO53_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET8875582.1"
                     /translation="MIDLVIEARSRDLGGFTVGRVLPFAKRRSVGPFVFFDHFGPLDM
                     PAGIPRSVDVRPHPHIGLSTLSYLFEGEIMHRDSLGSEQAIRPGEVNWMTAGRGITHS
                     ERFERARAQGGPMHGIQAWVGLPVEHEEIEPSFAHYASDALPTFESGGLWARLVAGEA
                     FGARASVATYSPLFYVHWQLQPQARAQLPAEYSERAAFVASGVVEVDGQRASAGQMLV
                     FAPGAPITFTASTQSTVMLLGGEPIGERYLEWNFVSSSRARIEQAKADWRAGRMKLPV
                     HDDAEFIPLPNDPPPPPNPMS"
     gene            complement(2925..3950)
                     /locus_tag="VFO53_04575"
     CDS             complement(2925..3950)
                     /locus_tag="VFO53_04575"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET8875583.1"
                     /translation="MSNGNELRATIGAIVAAGKGILAADESTPTITKRFQAVGIESSA
                     ESRRAYRTLLATAPGAQDYLGGVILFEETLGQAADDGTPLPRLLADHAILPGIKVDKG
                     TTPLEGAPGDLITQGLDGLAERLAAYKVQGARFTKWREVYGITDRNPTPLGISANAEV
                     LARYAAICQAAGLVPIVEPEVLIDGDHTLERCYEVSEAVLHAVFDALHRHRVALEGIV
                     LKPNMVLPGKGNARKASPDEVAAATLDVLRRTVPAAVPTINFLSGGQAPEEATANLNA
                     INAMDPRAPWVVSFSFARALQDPVMHVWKGEARNVAAAQQAFVHRLRMNSLAREGKWS
                     PAMERQH"
     gene            3986..>4464
                     /locus_tag="VFO53_04580"
     CDS             3986..>4464
                     /locus_tag="VFO53_04580"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8875584.1"
                     /translation="MTCEVYPGRRGVGAIRPRIEPGCGHATLAFVAARLPSFVSRSFA
                     SSPLRNATFRNFYIGSIGAALGYTMQATISAWLMATLTTSALMVALVQSASTAPTLLF
                     GLFAGTLADIFDRRRVILVTQVLLFAASLLLGAAALAGVVGPVTLLFLTFLVGAGFT"
BASE COUNT          714 a         1551 c         1507 g          692 t
ORIGIN      
        1 accccacacg gctcacctcg tgcgaatacg cttcgcgcgt gatcgcgtcg gcaccggcgc
       61 gctcgggtgc gcgccgcccc gagaaacggg tgaccgcgag gtcgaggctg ccccagaaga
      121 agtgaacggg actgcacttg ccgatgaaat ccgcgcgaaa tacgttgaag acgcgctcga
      181 cgctacgcag cacgcgccag aatcgctgca cggcgtcgcg atcgtagctt gcgtgcgtgc
      241 ggtcctgcgc gaacgcgatc ggatcgggca cctcgaccgg catcggccag atcgcgacgt
      301 cgatgtcggc cccatgcagc atttcgatga acgagtgata aaaatcggcc acggtgcgcg
      361 gcgcgagcgg catcgtgcgc accatgccct cgctcgtctc gagaacgagc tcgtgccgga
      421 cgaaatcgaa ttgcagctcg agcgtgcgac cccggtacgg catcgccgac gtcgtgagcc
      481 cgcgcgcatt caggtagagc gtcacgttcc accaatggtt cacgagcggt gccgttgcga
      541 gacgcagctt gccgacgatc tgcgtccaca tgtgcagcgt gtcgcacgtg tccttccagt
      601 cggcgagcgc cagggcgggc cagcactcgg tcgcgtcgtc ctgcggctcg catgcacccg
      661 cggatggccg ggaagggttc gccgctgcaa cttcagtcat cgttgcgctc ccgaagatcg
      721 ttggcgcctg cgcatagcgt acaccgcggc gcctgccagc agcagcacca gagcctcgag
      781 gatcgattgc cagggcaggc tgatggcaac gagcgcacag ccggcaaggc cggcgatcgc
      841 gagcgcacgc ggccagcgcc gctcgtcgcg gcgcagcgtg acagcggatg cgttcgcgat
      901 cgcgtaatag acgagcacgg caaacgagct gaagccgatc gcatcgcgaa catccgcgat
      961 caggacgatc agcgcgacga tcgcaccggc cgcgagctcg gcgtggtccg gtacccggtg
     1021 tcgcgcgtgc accgacgcga gaaagcgcgg caggtcgcgg ttccccgcca tcgcaaacgc
     1081 cgtccggctg acgcccgcca gcagcgataa aaggaccccc agcgacgcga tggccgcacc
     1141 gatgcgcacc gccggcgcaa gcgccgacca gcgtccgctt tcgacaacgt gcaccagcgg
     1201 cgcacgcgtg ccggcgagat cgcgggcgtc gacggtgacg agcgcgctga tggcgacggc
     1261 ggcgtagacg agcagcgtga gaccgagggc cagcggaatc gcgcgcggaa tggtacgcgc
     1321 cgggtcgatc acctcttcac cgagcgtcgc gatgcgtgca taccctgcaa aggcgaagaa
     1381 gaggaaaccc gccgcctgca ggatcccgcg gatgccgggc gcactccccg gtatcgcgcg
     1441 tgtgacgtcg acgttgccgc caaacgcggc cgcgaatacg acgacggcga ggcaggccag
     1501 caccaccgcg acgatcacgc gcgtcgcaag gacggttcgc gagatgccga aatagttgac
     1561 cgcggtcagc gcgatcaccg ccaggatggc gagcggccgg gaaaacgcgg gcgcggcata
     1621 cgcagcgaat gtcagcgcca tcgcggcgca gctcgcgagc ttgccggtca cgaagcccca
     1681 gccggcgagg aagccccaga acgcacccaa tcgcctgcgg ccgtagacgt acgtgccgcc
     1741 cgattccgga tacagcgcag cgagtgccgc ggacgacgtc gcattgcagt aagcgacgaa
     1801 cgccgccacc atgagtccaa ccagcagcgc cggacccgcc gccgccgcgg ccggcgcgag
     1861 tgcggcgaag atgcccgcgc cgatcatcga tccgaggccg atgacgactg catcggcgac
     1921 gccgagcttg cgcagaaggc gcgtcgccgg tgcgctcggc gcggtcgtgg gtccgctcat
     1981 cgtcgctgcg tcatacttgc aagacccaca gcagggagcc cgacatgatc gatctcgtca
     2041 tcgaagcacg cagccgcgac ctcggcgggt tcaccgtcgg gcgcgttctt ccgttcgcga
     2101 agcgccgcag cgtcggcccg ttcgtgttct tcgaccattt cggcccgctc gacatgccgg
     2161 ccggcatccc gcgcagcgtc gacgtgcggc cgcatccgca tatcggcctg tcgacgcttt
     2221 cgtacctgtt cgaaggcgag atcatgcatc gcgacagcct tggctcggag caggcgatcc
     2281 ggcccggcga agtcaactgg atgacggccg gccgcggcat cacgcattcc gagcgcttcg
     2341 agcgcgcgcg tgcacagggc ggaccgatgc acggcatcca ggcgtgggtc ggtttgccgg
     2401 tcgagcacga ggaaatcgag ccgtcgttcg cgcattacgc gtccgacgcg ctgccgacgt
     2461 tcgaaagcgg cggcttgtgg gcgcggctcg tcgccggcga ggcgttcggc gcgcgcgcaa
     2521 gcgtcgccac ctattcgccg ctcttctacg tgcactggca actgcagccg caggcgcggg
     2581 cgcaattgcc ggccgaatat tccgagcgcg ccgcattcgt cgcgagtggc gtcgtggagg
     2641 tcgacgggca gcgcgcgagc gccggccaga tgctcgtctt cgcgccgggc gcgccgatca
     2701 cgttcaccgc gtcaacgcag tcgacggtaa tgctgctcgg cggcgagccg atcggcgagc
     2761 gctatctcga gtggaatttc gtgtcgtcat ccagggcgcg catcgagcag gcgaaggccg
     2821 actggcgcgc gggccgcatg aagctgccgg tccacgacga cgccgaattc attccgctgc
     2881 cgaacgatcc gccaccgccg cccaatccga tgtcgtgaac ggcgtcagtg ctggcgctcc
     2941 atcgccggcg accacttgcc ttcgcgggca aggctgttca tccgcagccg gtggacgaac
     3001 gcctgctgcg ccgccgcgac gttgcgcgcc tcgcctttcc agacgtgcat caccgggtcc
     3061 tgcagcgcac gcgcaaagga gaacgacacc acccacggtg cgcgcgggtc catcgcgttg
     3121 atcgcattca gattcgcggt ggcctcctcg ggcgcctgtc cgccggaaag gaagttgatc
     3181 gtcggcaccg cggccggtac cgtgcggcgc agcacatcga gcgtggcggc cgcaacctcg
     3241 tccggcgacg ccttgcgcgc gttgcccttg cccggcaaga ccatgttcgg cttcagcacg
     3301 atgccttcca gcgcgacgcg gtgccgatgc aacgcatcga acaccgcgtg cagcaccgct
     3361 tccgacacct cgtaacaacg ctcgagcgtg tgatcgccgt cgatcagcac ctcgggctcg
     3421 acgatcggca cgaggccggc tgcctggcag atcgctgcgt agcgcgcgag cacttcggca
     3481 ttcgcactga tgccaagcgg cgtcggattg cgatcggtga tgccgtagac ctcgcgccac
     3541 ttcgtgaacc gcgctccctg gaccttgtac gcggcgagcc gttcggcaag gccgtcgagg
     3601 ccctgcgtga tcaggtcgcc gggcgcacct tcgagcggcg tcgtgccctt gtcgaccttg
     3661 atgcccggca ggatggcgtg atcggcgagc agccgcggca atggcgtgcc gtcgtcggcg
     3721 gcctggccga gggtttcctc gaacaggatg acgccgccta gatagtcctg cgcgcccggt
     3781 gcggtggcga ggagcgtgcg gtacgcccgg cgcgattcgg ccgacgattc gatgcccacc
     3841 gcctggaagc gcttggtgat cgtgggcgtg ctctcgtcgg ccgcgagaat gcccttgccc
     3901 gccgcgacga tcgcaccgat cgtcgcgcgc aattcattgc cgttgctcat cgctttctcc
     3961 ttggctgcgg cgctgcgttc gccgcatgac gtgcgaagtt taccccggcc gtcgcggcgt
     4021 cggagcgatc cgtccgcgca tcgagcccgg gtgtggtcac gctacacttg ccttcgtggc
     4081 cgcgcgcctg ccttcgttcg tctcccgctc gttcgcatcg tcgccgctcc gcaacgcgac
     4141 gtttcgcaac ttctacatcg gctcgatcgg cgcggcgctc ggctacacga tgcaggcgac
     4201 gatttcggcg tggctcatgg ccacgctcac gacgtcggcg ctgatggtcg cgctggtgca
     4261 gagtgcgagc accgccccga cgctgctgtt cggcctcttc gccggaacgc tcgccgacat
     4321 cttcgaccgg cgccgcgtga tcctcgtgac gcaggtgctg ctgttcgccg cgagcctgct
     4381 gctcggcgcg gctgcgctcg cgggcgtcgt cgggccggtg acgctattgt tcctgacgtt
     4441 cctcgtcggc gcgggcttca cgtt
//
LOCUS       DASSVX010000100         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1586739, whole genome shotgun sequence.
ACCESSION   DASSVX010000100 DASSVX010000000
VERSION     DASSVX010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586739"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1509
                     /gene="leuC"
                     /locus_tag="VFO53_04585"
     CDS             100..1509
                     /gene="leuC"
                     /locus_tag="VFO53_04585"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET8875585.1"
                     /translation="MHARTLYDKLWDSHLVREEGDGTALMYIDRHLVHEVTSPQAYEG
                     LRLARRSPWRVESIVATADHNTPTTHWDEGIRDPISRTQVEALDENIRRYGAKAYFPF
                     RDRRQGIVHVIGPENGATLPGMTVVCGDSHTSTHGAFAALAFGIGTSEVEHVLATQCL
                     LTRKSKAMLVRADGRLSPGVTAKDLVLSVIGRIGTAGGTGHAVEFAGDAVRALSMEGR
                     MTMCNMSIEAGARAGMVGVDERTIDYLKGRTYAPTGELWTRAVAYWRTLATDAGAAFD
                     TTHVFDAASVKPQVTWGTSPEMVASIDDRVPDPERERDAERREGIARALDYMGLAPNV
                     RIADIRIDKVFIGSCTNSRIEDLREAANVVRGRHVAGSVRLAMVVPGSGLVKAQAEAE
                     GLDRTFRDAGFEWREPGCSMCLAMNDDRLEPGERCASTSNRNFEGRQGAGGRTHLVSP
                     AMAAAAALAGHFVDVRRLS"
     gene            1521..2156
                     /gene="leuD"
                     /locus_tag="VFO53_04590"
     CDS             1521..2156
                     /gene="leuD"
                     /locus_tag="VFO53_04590"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET8875586.1"
                     /translation="MQPFTVHRGIVAPLDRANVDTDAIIPKQFLKSIRRSGFGVNLFD
                     AWRYTDRGEPGMEQARRHPNPDFVLNAPRYKGASILLARRNFGCGSSREHAPWALADY
                     GFRALIAPSFADIFFNNCFKNGVLPIVLPDAKVDRLFADVAAFPGYELTIDLERQTVG
                     SVDGGLSFPFDVDPFRKRCLLNGWDDIGLTLQHADEIRAFETRHLAEQPWL"
     gene            2231..>3164
                     /gene="leuB"
                     /locus_tag="VFO53_04595"
     CDS             2231..>3164
                     /gene="leuB"
                     /locus_tag="VFO53_04595"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET8875587.1"
                     /translation="MKIAILPGDGIGPEIVREAERVLDVLASEGMPIERARGAIGGAG
                     YESAGHPLPPSTLDLAHAADAVLLGAVGGPKYDALPRELRPEQGILGLRKALALFANL
                     RPALLYPELAASSSLKADVIAGLDLVIVRELTGDIYFGEPRGRRSNAAGEREGFDTMH
                     YAEGEIARIARVGFETAMKRRRQLVSVDKANVLDTSILWREVVNEVARDYPDVALSHM
                     YVDNAAMQLVRRPKSFDVIVTGNLFGDILSDEASMLAGSIGMLPSASLDASGKGLYEP
                     IHGSAPDIAGQDKANPLATILSLAMMFRYTFASAE"
BASE COUNT          522 a         1038 c         1079 g          525 t
ORIGIN      
        1 gtggtggggc gtacaaagcg aataaagcaa cgtccgggcc atccgcttcg cacgcactag
       61 aatgacagct ttcccgatct tccggcatcg cgaaagccca tgcacgcccg caccctttac
      121 gacaagttgt gggacagtca cctcgtacgc gaagagggcg acggcaccgc gctgatgtac
      181 atcgaccgtc acctggtgca cgaggtgacg agcccccagg cgtacgaagg gttgcgcctc
      241 gccaggcgca gcccgtggcg cgtcgagtcg atcgtcgcca ccgccgatca caacacgccg
      301 acgacccatt gggacgaagg cattcgcgat cccatctcgc gcacgcaggt cgaagcgctc
      361 gacgagaaca tccgccggta cggggcgaag gcgtattttc cgtttcgcga tcggcgccag
      421 ggcatcgtgc atgtgatcgg gccggaaaac ggcgcgacgc tgcccggcat gaccgtcgta
      481 tgcggcgatt cgcatacgag cacccacggc gcattcgcgg cacttgcatt cggtatcggc
      541 acgtccgagg tcgagcacgt gctggccacg cagtgcctcc tcaccaggaa gtcgaaggcg
      601 atgctggttc gcgccgacgg ccgtctgtcg ccaggcgtca cggcgaagga tctcgtgctg
      661 tcggtcatcg gacgcatcgg caccgcgggc ggaaccggtc acgcggtcga gttcgcgggc
      721 gacgccgtgc gcgcgctgtc gatggaaggc cggatgacga tgtgcaacat gtcgatcgag
      781 gccggtgcgc gcgccggcat ggtcggcgtc gacgagcgca cgatcgacta cctgaaggga
      841 cgcacgtacg caccaacggg cgagctgtgg acgcgtgccg tcgcgtactg gcgcacgctc
      901 gcaaccgatg cgggtgccgc attcgacacg acgcacgtgt tcgacgcggc ctcggtcaag
      961 ccgcaggtca cgtgggggac gtcgcccgaa atggtcgcgt cgatcgacga tcgggtgccc
     1021 gaccccgagc gtgaacgcga cgccgagcgg cgcgaaggca tcgcgcgggc gctcgattac
     1081 atgggtctcg cgccgaacgt gcggatcgcc gacatccgca tcgacaaggt gttcatcggc
     1141 tcgtgcacga actcgcgcat cgaggacctg cgcgaggcgg cgaacgtcgt gcgcggtcgc
     1201 cacgtcgccg gcagcgtgcg ccttgcgatg gtggtgccgg ggtcgggcct cgttaaggcg
     1261 caagccgaag ccgaaggcct cgatcgcacg tttcgtgacg ccggcttcga gtggcgcgag
     1321 ccgggctgct cgatgtgcct cgcgatgaac gacgatcgtc tcgagccggg cgagcgctgc
     1381 gcgtcgacgt cgaaccgcaa tttcgaggga cgccagggcg cgggagggcg cacgcacctg
     1441 gtgagtccgg caatggcggc ggcagccgcg cttgccgggc atttcgtcga cgttcgtcgg
     1501 ctttcgtaga gcgattggcg atgcagccgt tcaccgtcca ccgggggatt gtcgcgccgc
     1561 tcgaccgcgc caacgtcgac accgatgcga tcatccccaa gcagttcctg aagtcgatcc
     1621 gccgttcggg attcggcgtc aacctgttcg atgcctggcg ctacaccgat cgcggcgagc
     1681 cgggaatgga gcaggcgcgg cggcatccga atcccgattt cgttctcaac gcgccgcgtt
     1741 acaagggtgc gtcgatcctg ctcgcgcgtc gcaacttcgg ctgcggcagc tcgcgcgagc
     1801 atgcgccgtg ggcgctggcc gattacggct ttcgcgcgct gatcgcaccg tcgttcgccg
     1861 atatcttctt caacaactgc ttcaagaacg gtgtgctgcc gatcgtgctc cccgacgcga
     1921 aggtcgaccg cctgttcgcc gatgtcgcgg cgttcccggg ctacgagctc acgatcgatc
     1981 tcgagcgtca gacggttggc agcgtggacg gcgggctttc gttcccgttc gacgtcgatc
     2041 cgttccgtaa gcgctgtctg ttgaacggct gggacgacat cggcctcacg ctgcagcacg
     2101 ccgacgagat ccgcgcgttc gagacgaggc atctcgccga gcagccgtgg ctgtaggggt
     2161 cagagacgac ttttgcgcat gggccggccg accatcgtga cacgaccttc gcaaagtcgt
     2221 ctctgacccc atgaagattg cgatcctgcc gggtgacggc atcggccccg agatcgtccg
     2281 cgaggccgag cgcgtcctcg acgtgctcgc gagcgagggg atgccgatcg aacgtgcacg
     2341 cggggcgatc ggcggcgcgg gctacgaaag cgccggccac ccgctgccgc cttcgacgct
     2401 cgatctggcg cacgccgccg acgccgtgct gctgggcgcg gtcggcggac cgaagtacga
     2461 cgcgctgccg cgcgagctgc gtcccgagca gggcatcctc ggactgcgca aggcgctcgc
     2521 gctgtttgcg aatctgcggc cggcgttgct ctatcccgag ctcgcggcct cctcgtcgct
     2581 caaggccgat gtcatcgccg gtctcgatct cgtgatcgtg cgcgagctga cgggtgacat
     2641 ctatttcggc gagccgcgcg gacgccggtc gaatgccgcc ggcgagcgcg aaggcttcga
     2701 cacgatgcac tatgcggaag gcgagatcgc ccgcatcgcg cgcgtcggct tcgagacggc
     2761 aatgaagcgc aggcggcagc tggtatcggt cgacaaggcg aacgtgctcg acacgtcgat
     2821 cctctggcgc gaggtggtga acgaggtcgc gcgcgactat cccgacgtcg cgctttcgca
     2881 catgtacgtc gacaatgccg cgatgcagct cgtgcgccgc ccgaagagct tcgacgtcat
     2941 cgtcaccggc aatctgttcg gggacatcct gtccgacgag gcgtcgatgc tcgccggatc
     3001 gatcggaatg ctgccgtcgg cgtcgctcga tgcgagcggc aagggcttgt acgagccgat
     3061 ccacggctcg gcgcccgata tcgccggaca ggacaaggcc aatccactgg cgacgatcct
     3121 gtcgctcgcg atgatgttcc gctacacgtt cgccagtgcc gaag
//
LOCUS       DASSVX010000101         5303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_720120, whole genome shotgun sequence.
ACCESSION   DASSVX010000101 DASSVX010000000
VERSION     DASSVX010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720120"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VFO53_04600"
     CDS             <1..1344
                     /locus_tag="VFO53_04600"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HET8875588.1"
                     /translation="ILFEIYYAEVAPVASSRDVHASIAALDKLRTVLNPARRMDVAYF
                     KFQESTVRMLEGRADEAVTAARDAVTIGRSAGLPTMQIPHFLVREALSNLQRGAIDEA
                     LARYDEALEIAVGVDADNFREQRGLVVAYAALRQNRVDDAVASLRERLRACRERRYTG
                     FLRQLPQMMAELLALALAHGIEPEFTRALIRERRLEAPSPATIDWPWPLALRTLGDFI
                     VIRDGEPLVSKGKAQKRPLEMLKALIALGGRHVDASMLTAKLWPDAEGDDAKTSFDSG
                     LYRLRKLLDVDGALTLAEGRLSLDDRLVWLDVWAFEQALDATPPDIDAALACYRGHFL
                     GLEAPAPWALPLRDRLQARLARAVLARGQALEADKKFAAARTLYERALELDNLAEAVY
                     RRLMVCQRELGDPAGALTTYRRCRELLSIVLGRKPAAETEAIRATLDRVATQGDG"
     gene            1341..2024
                     /locus_tag="VFO53_04605"
     CDS             1341..2024
                     /locus_tag="VFO53_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875589.1"
                     /translation="MTATARFLRHVDTARKTQLPIRIAEATPQTLALWDAHVQPHVRN
                     AREFRFDRKWQWPALLKFHQVQEAIIGLAGARPAADVRLLQAFAERTDGTAFPVGQVL
                     LACGFPFIRDWDAPSVFLWYLAGAPRQALHAAGLAPDLKLMRPLVDIAVQESFARGFE
                     GRIGLHAARSRWNLAANRDLYNRYRHIGLAPLPGGLRASVGRRNDRRYFYLDAPLALD
                     FARRLDYLR"
     gene            2029..2325
                     /locus_tag="VFO53_04610"
     CDS             2029..2325
                     /locus_tag="VFO53_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875590.1"
                     /translation="MAAITHVRNQHAVLTPESGDDLGERLAALAADLFSADAATLAVE
                     RAFLDRLLRILALTPHEVHDLAVAGRLTGMLPPDTPGAAEPIRFADLAGWRDPK"
     gene            2430..3332
                     /locus_tag="VFO53_04615"
     CDS             2430..3332
                     /locus_tag="VFO53_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875591.1"
                     /translation="MFVVPVSHSSPAVSTHRRPPAARREGLTSLRGFPFPVLSSELAT
                     AAATRIALRAQDASDLLRSVLGVTPRLSLRVLDRRNWAAHAEVAAYGVTHVATNGDLI
                     VGATPPDAWHGVSRYLARRLSAHELARLVDVHGVDSTHSRGPALDALAESLIIHDVAH
                     VLADQHGLEFPSRWLEEAFANYVLVAVLGTNDPQGLRLLGSLAEAARSLDDGLPTLAD
                     FERGTSRTDVASSVLAELAITRAVYAAYASRDTAPLVDLFESVRTSDADADFELGRMA
                     ALPLYPSLAAIPSLFPATHVGLAA"
     gene            3367..3780
                     /locus_tag="VFO53_04620"
     CDS             3367..3780
                     /locus_tag="VFO53_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013524822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase"
                     /protein_id="HET8875592.1"
                     /translation="MSARLIKVALLYLVVGVTMGIVMGITHQFVLAPVHAHINLLGFA
                     TLAIVGVIYHAYPAAAQTRLAAVHFWIHTIALPVFMVGLGFALTGHEAFVAVTSIGAT
                     GVAIGIIVLAINIWRTVVPAVRAKSQALPSARATA"
     gene            3975..>5303
                     /gene="ubiB"
                     /locus_tag="VFO53_04625"
     CDS             3975..>5303
                     /gene="ubiB"
                     /locus_tag="VFO53_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HET8875593.1"
                     /translation="MRLGRLFRIVVVALRHGLDDLALGHARVRAFRPIVRTVLFWRDV
                     SLPRGVRLRRALEDLGPIFIKFGQMLSTRRDLLPPDIADELALLQDRVPPFSSDLVEA
                     TIVRVFGRPLADIFRTFDLTPVASASVAQVHFAELPDGTPVAVKVLRPNVADVIEHDL
                     ALMRTAASLVERLWSEGKRLRPREVVAEFDKTIHDELDLTREAANCSQLRRNFLRSGL
                     MRVPEIYWDWTASEVLVMERMAGIPIGRIAELTAAGIDFKRLARAGVEIFFTQVFRDG
                     FFHADMHPGNIFVGVDAANHGKYIALDFGIMGTLSEQDKNYLAQNFLAFFRRDYHRVA
                     TTHIESGWVPAATRVDELESEIRVVCEPIFDRPLKEISLGKVLVQLFRASRRFNVEIQ
                     PQLVLLQKTLLNVEGLGRQLDPDLDLWVTAKPFLERWMEDQIGFRALERRL"
BASE COUNT          808 a         1821 c         1726 g          948 t
ORIGIN      
        1 attctgtttg agatttatta cgcggaggtg gcgccggtcg cttcttcgcg tgacgtccac
       61 gcgtcgattg ccgcactcga caagttgcgc accgtgctga acccggcgcg acggatggat
      121 gtcgcgtact tcaagttcca ggagtcgacg gtgcgcatgc tcgaagggcg tgccgatgag
      181 gccgtcaccg ccgcgcgcga tgcggtgacg atcggccgca gcgccggcct gccgacgatg
      241 cagattccgc atttcctcgt gcgcgaggcg ttgtcgaacc tgcagcgcgg cgcgatcgac
      301 gaggcgctcg cgcgatacga cgaggcgctc gaaatcgccg ttggcgtcga cgccgacaat
      361 tttcgcgagc agcgcggcct cgtcgtcgcg tatgcagcac tgcggcaaaa ccgcgtcgac
      421 gatgccgtcg cgtcactgcg cgagcgactg cgcgcgtgcc gcgagcgtcg ctataccgga
      481 ttcctgcgcc agctgcccca gatgatggcg gaattgctcg cgctggcgct cgcgcatggc
      541 atcgagcccg agttcacgcg cgcgctgatc cgcgagcggc ggctcgaggc gccgtcgcct
      601 gccaccatcg actggccgtg gccgcttgcg ttgcgaacgc tcggcgattt catcgtcatc
      661 cgcgacggcg agccgctcgt gtcgaaaggc aaggcgcaga agcggccgct tgaaatgctg
      721 aaggcgctga tcgcgctcgg cggccgccat gtcgacgcat cgatgctgac cgcgaagctc
      781 tggcccgacg ccgaaggcga cgacgcgaaa acgtcgttcg acagcgggct ctatcggttg
      841 cgcaagctgc tcgatgtcga cggcgcgctc acactcgccg aaggacgtct gtcgctcgac
      901 gatcggctcg tatggctcga cgtctgggcc ttcgagcagg cgctcgacgc aacgccgccc
      961 gacatcgatg cggcgctcgc gtgctatcgc ggccattttc tcggactcga agcgcccgcg
     1021 ccgtgggcgc tgccgctgcg cgatcggctg caggcgcgac tcgcacgggc ggtgctcgcc
     1081 cgcggtcagg cactggaggc ggacaaaaaa ttcgccgcgg cgcgcacgct gtacgaacgc
     1141 gcgctcgagc tcgacaacct ggccgaagcc gtctaccggc gcctgatggt gtgccagcgg
     1201 gagctcggcg atccggcggg cgcgctgacg acgtaccgcc gctgccgcga gctcctgtcg
     1261 atcgtgctcg gccgcaagcc ggcagcggaa accgaggcga tccgcgcgac gctcgatcgc
     1321 gtggccacac agggcgacgg gtgacggcaa cagcacgctt tctgcgccac gtcgacacgg
     1381 cgcggaagac gcagttaccg atcaggattg ccgaggccac cccgcagacg ctcgcgctgt
     1441 gggacgcgca cgtgcagccg cacgttcgca acgcgcgcga gtttcgcttc gaccgcaaat
     1501 ggcaatggcc ggcgttgctc aaattccatc aggtgcagga agcgatcatc ggcctggccg
     1561 gcgcacgccc agccgccgat gttcgcttgc tgcaggcgtt cgccgagcgc accgacggca
     1621 cggcgtttcc cgtcggtcag gtgttgctcg catgcggttt tccgttcatt cgcgattggg
     1681 atgcgccatc ggtgttcctc tggtacctgg cgggggcgcc gcggcaggcg ctgcacgcgg
     1741 cggggctcgc acccgatctc aagctgatgc gcccgctcgt cgacatcgcc gtccaggaaa
     1801 gcttcgcccg cggcttcgaa ggccgcatcg gccttcatgc ggcacggagc cggtggaacc
     1861 ttgcggcgaa tcgcgatctc tataaccgat accggcacat cgggttggca ccgctgcccg
     1921 gcggattgcg cgccagcgtc ggaaggcgca acgaccgtcg gtacttctat ctcgatgcgc
     1981 cgctcgcgct ggactttgcg cgacggctcg attaccttcg ttgacgccat ggccgccatc
     2041 acgcacgttc gcaaccagca cgccgtattg acgccggagt ccggtgacga cctcggcgaa
     2101 cggcttgcag cgctcgccgc cgatctcttt tcggcggacg ccgcgaccct cgctgtcgaa
     2161 cgagcgttcc tcgaccggtt gttgcgcatc cttgcgctga cgccgcacga ggtgcacgac
     2221 ctcgctgtcg cgggccgttt gaccggcatg ctgccaccgg acacgcccgg cgccgccgaa
     2281 ccgatccgtt tcgccgatct cgccggctgg cgcgacccga agtagcgtcc gccgccggct
     2341 ccggcggcct ccttatcgat ccgtcagtga tccgtgagcc tgcggaggcg acgatgcggg
     2401 cgtagcgaac caacgcccga ggagccgcaa tgttcgtcgt ccccgtatcg catagttccc
     2461 cagccgtttc cacgcatcgg cgtccgccgg cagcgcgccg cgaaggcctg acgtcgctgc
     2521 gcggctttcc attccccgtg ttgtcgagcg agctcgcgac cgcggcggcg acgcgcatcg
     2581 cactgcgcgc gcaggacgcg tccgatttac ttcgcagcgt gctcggtgtc accccccggc
     2641 tttcgctgcg cgtgctcgat cgacgcaact gggctgcgca cgccgaagtt gccgcgtacg
     2701 gcgtcacgca cgtggccacc aatggcgatc tcattgttgg cgcgacaccg cccgacgcat
     2761 ggcatggcgt cagccgctat ctcgcgcggc ggctgtccgc gcatgagctc gcgcgtctcg
     2821 tcgatgtgca tggcgtcgat tcgacacact cgcgcggtcc cgcactcgat gcattggcgg
     2881 aatcgctgat catccacgac gttgcgcacg tgctcgccga ccagcacggg ctcgagttcc
     2941 cgtcgcgctg gctcgaggaa gcattcgcga attacgtgct cgttgccgtg ctcggcacga
     3001 acgatccaca ggggctgcga ttgctgggct cgctcgcgga ggctgcgcgc tcgctcgacg
     3061 atgggctgcc aacgctcgcc gacttcgagc gcggcacgag tcgcaccgac gtcgcatcat
     3121 cggtgctcgc cgagctcgcc atcacgcgcg ccgtgtatgc ggcctacgcg tcgcgcgaca
     3181 cggcgccgct cgtcgatctg ttcgaaagcg tgcgtacgag cgacgccgac gcggatttcg
     3241 agcttggccg gatggccgcg ctgccgctgt atccgtcgct cgccgcgatc ccgtcactat
     3301 ttccggcaac gcacgtcggc ctcgccgcct gaattcaaca cgatcatctt cgaaggaacc
     3361 acgccaatga gcgcacgctt gatcaaggtt gcactgctgt atctcgtcgt cggtgtgacg
     3421 atgggaatcg tcatgggcat cacgcatcag ttcgtgctgg cgcccgtcca cgcccacatc
     3481 aatctgctgg ggttcgcgac gctcgcgatc gtcggcgtca tctatcacgc gtatcccgct
     3541 gcggcacaaa cgcgcctcgc tgccgtgcac ttctggattc acacgattgc actgccggtg
     3601 ttcatggtcg gtctcggctt tgcgctgacc ggtcacgagg cgttcgttgc ggtgacgagc
     3661 atcggtgcca ctggcgttgc gatcggcatc atcgttctcg cgatcaacat ctggcgtacg
     3721 gtcgtgccgg ccgtgcgcgc gaagtcgcag gccctcccga gtgcccgcgc aaccgcgtga
     3781 tgagtgccgt cgacaagttg cgccgaacgc ggcgcaagcc acccacgagc cccgcccagg
     3841 caccacgcgc cgcacaatcg tcgccagggg ctctccaaac gcctaccgct acgccgccaa
     3901 cgcgtaaacg ccgcccgcgc ttcgtcctgt agcgaaaccc ggcggaggca ggacgcgccg
     3961 gtacaatccg ccccatgcgg ctgggtcggc tcttccggat cgtcgtcgta gcgttgcggc
     4021 acggtctgga cgaccttgct cttggccatg cgcgcgtccg cgcgttccgg ccgatcgtgc
     4081 gcaccgtgct gttctggcgc gacgtttcgc tgccgcgcgg cgtccggctg cgacgcgcgc
     4141 tcgaggatct cgggccgatc ttcatcaagt tcggccagat gctgtcgacg cggcgcgacc
     4201 tcctcccacc ggacatcgcc gacgagctgg cgctcttgca ggatcgcgtg ccgccgttct
     4261 cgtcggatct cgtcgaagcg acgatcgtcc gtgtattcgg gcgcccgctc gccgacatat
     4321 tcaggacgtt cgacctgacg ccagtcgcca gcgcgtcggt cgcgcaggtt cacttcgcgg
     4381 agctgcccga cggtacgccg gtcgccgtca aggtgctgcg gcccaacgtc gccgacgtca
     4441 tcgagcacga tctcgcgctg atgcgaacgg cggcttcgct cgtcgagcgg ttgtggagcg
     4501 aaggcaagcg gttgcggcca cgcgaggtcg tcgccgagtt cgacaagaca attcacgacg
     4561 agctcgacct cacgcgtgaa gcagcgaact gctcacaact gcgacgcaat ttcctgcgct
     4621 cgggcctgat gcgcgtgccc gagatctact gggactggac ggcgagcgaa gtgctcgtca
     4681 tggagcggat ggccgggatt cccattggcc gcatcgcgga gttgaccgct gccggcatcg
     4741 acttcaagcg gctcgcgcgc gcgggtgtgg agatcttttt cacgcaggtt tttcgcgatg
     4801 ggttcttcca cgccgacatg catccgggca acatcttcgt cggtgtcgac gcggcaaacc
     4861 acggcaagta catcgcgctc gacttcggca tcatgggaac gctgtcggag caggacaaga
     4921 attacctggc gcagaatttc ctggcgttct tccgccgcga ctatcaccgc gtcgcgacga
     4981 cgcacatcga atccggctgg gtgccggcgg cgacccgcgt cgacgagctc gagagcgaga
     5041 ttcgcgtcgt ctgcgagccg atcttcgacc ggccgctcaa ggaaatctcg ctcggcaagg
     5101 tgctcgttca actgtttcgc gcgtcgcgtc gattcaacgt tgagatccag ccgcagctcg
     5161 tgctgctgca gaagacgctt ttgaatgtcg aggggctcgg ccgccagctc gaccccgatc
     5221 tcgacttgtg ggtaacggcg aagccctttc tcgagcgctg gatggaggat cagatcggct
     5281 ttcgcgcgct cgagcggcgg ctc
//
LOCUS       DASSVX010000102        10202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1653452, whole genome shotgun sequence.
ACCESSION   DASSVX010000102 DASSVX010000000
VERSION     DASSVX010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10202
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653452"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="VFO53_04630"
     CDS             <1..174
                     /locus_tag="VFO53_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET8875594.1"
                     /translation="ERIEIRGFGSFGLNYRPPRTGRNPKSGEKVQVPEKYVPHFKAGK
                     ELRERVDFNLVQD"
     gene            248..580
                     /locus_tag="VFO53_04635"
     CDS             248..580
                     /locus_tag="VFO53_04635"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HET8875595.1"
                     /translation="MSLLRWIFIAFVFIALLFISLQNADPVTLRLFSLYAWQAPLVFV
                     ILLAFATGVALGLLASAIKVVRLKRQLARLRREHHRAQSALPAARGPAPGTAAASGPG
                     FEPPSTGF"
     gene            588..1766
                     /gene="lapB"
                     /locus_tag="VFO53_04640"
     CDS             588..1766
                     /gene="lapB"
                     /locus_tag="VFO53_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HET8875596.1"
                     /translation="MDFELWWLLPIPALFFALGWIAARIDIKHLLHESRALPLSYFRG
                     LNFLLNEQPDKAIESFIEVVKVDPQTIDLHFALGSLFRRQGETERAIRMHQNLLDRPT
                     LPADKRTMATYELAQDFHRAGLLDRAENLFQRLDGTPFEHSALSHLIEIYETEKDWPK
                     AITAMQRMEVLAKQPYYKEIAEYHCELAQAALLKSDTPTARAEIERALAAHRGCARAN
                     LIAGDAEAQSGDFRAAVEAWQRIESQNPAFLALAADRFADAFRRLRDLPTGIRLLRNY
                     QAQYSSLDLLNALFTLTLEQEGADAAATLIKDELARNPTLIGLDRLLEAQLLTSPADR
                     RHDLELVKGLVGQHIKRLGLYKCDNCGFRARQYYWRCPGCQKWETYSPKRTEAPDGYT
                     "
     gene            1802..2524
                     /gene="pyrF"
                     /locus_tag="VFO53_04645"
     CDS             1802..2524
                     /gene="pyrF"
                     /locus_tag="VFO53_04645"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004861062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET8875597.1"
                     /translation="MTRANPRVLVALDFANPMRALALADRLDPAECGVKVGKELFVAA
                     GPEPVRWLVAHGFNVFLDLKFHDIPNTVAHACAAATRLGVWMVNVHAAGGSAMLRAAR
                     EAVAATAGETGIARPLLIAVTVLTSLDAAALGETGVDAEPGDQALRLAALAQACGLDG
                     VVCSAREAAAMRARFGPDFALVTPGIRPRGSPGDDQARIVTPEEAVRNGASYLVVGRP
                     ITGAADPPAALASINRALGIGA"
     gene            2521..3843
                     /locus_tag="VFO53_04650"
     CDS             2521..3843
                     /locus_tag="VFO53_04650"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET8875598.1"
                     /translation="MKITMIGTGYVGLVTGTCFAEVGNDVLCLDVDARKIDMLESGGV
                     PIHEPGLEPMIRRNVAAGRLRFTTDVDAAVGHASVQFIAVGTPPDEDGSADMQYVLQA
                     ARNIGRRMTQWTLVVDKSTVPVGTGDRVRAVIAEELAARGLDVPYAVASNPEFLKEGA
                     AVEDFMKPDRVVIGADNERAIAILRNIYAPFQRNRERLLVMDVRSAELTKYAANAMLA
                     TRISFMNELANLADRLGADIESVRQGIGSDPRIGWHFLYPGVGYGGSCFPKDVRALQH
                     TAARAGAPLKVLSAVEAANDAQKQVLVDKIVARFGNDLSQRRFAVWGLAFKPNTDDMR
                     EAPSRVVIAALTRRGATICAYDPVAMDEARRLLGDMPRVDFATSPLAACDGADALVVV
                     TEWKEFRSPDFDGLHRRMKQPLIFDGRNLYEPDVVRAAGFEHVAIGRR"
     gene            3980..4918
                     /gene="rfaE1"
                     /locus_tag="VFO53_04655"
     CDS             3980..4918
                     /gene="rfaE1"
                     /locus_tag="VFO53_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HET8875599.1"
                     /translation="MSAHFSAWRTRVAGAQVLIVGDVMLDRYWFGDVERISPEAPVPV
                     VKIARSEERPGGAANVARNIAALGARAMLLSATGRDEAADTLLRLLDSDRIDVSFLRD
                     EALKTTVKLRVIGRQQQLLRIDFETAPPHELLAAHLAEFDHLLGSADALVLSDYGKGG
                     LAHIATMIERGRAAGKPVLVDPKGDDWERYRGATLITPNRSEFRQVAGRARDEDDLVR
                     RAQTLRRDLDLDALLLTRSEEGMSLFSDAGALTIPAQAREVFDVSGAGDTVIATLGTL
                     LAAGAPMADAMAIANEAAGIVVGKLGTAVAEPDELR"
     gene            4961..6316
                     /gene="radA"
                     /locus_tag="VFO53_04660"
     CDS             4961..6316
                     /gene="radA"
                     /locus_tag="VFO53_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET8875600.1"
                     /translation="MAKPKTLYSCTECGGQAPKWQGQCPQCGAWNTLVETLAAPAAAR
                     FESVAGARSTVTQLSSVTPRATTRIATGLEEFDRVLGNGLVAGGVILLGGDPGIGKST
                     LLLQAGAALGALHRTLYVTGEESAEQIALRAQRLGLVNAPIELLAEVQLEAIVAAINA
                     TAPEIVVIDSIQTTYTETLASAPGSVAQVRECAAQLTRLAKQRGVVVIFVGHVTKEGA
                     IAGPRVLEHIVDTVLYFEGDPHSSFRLVRAIKNRFGAANELGVFAMTERGLRGVANPS
                     ALFLSQHAEGVAGSAVVATLEGSRPLLVEVQALVDPVQGGMPRRLAVGLDPQRLALLL
                     AVLHRHGGVETSGFDVFVNAVGGVRILEPAADLAVMLAVCSSLRNSALPKKSLIFGEV
                     GLAGEVRPVQRGQERIREAAKLGFETFVIPNLNKPKQRIDNVNIIGVDRVDEAIGWLR
                     R"
     gene            complement(6332..8431)
                     /locus_tag="VFO53_04665"
     CDS             complement(6332..8431)
                     /locus_tag="VFO53_04665"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8875601.1"
                     /translation="MSITSEFDRAYRLHQEGKLREAFMRYDAVLAADPKNAPALHYSG
                     VVLYQAGKIPEAAERIRASLQLEPASSEAWSNLALVLESAGRREAAVNALHEAAKIAP
                     RSPEILANLAAAELALGHIAEAEAAARNAVDVDARHAAGWHNLALVLEPQGRLLEAMD
                     ASSRASTLAPLEAAYAGFKAQLEATAGMRKKARATLDAALTRKPESPALRFQLAGLLE
                     RDGELERAMQAYEAVVRLDPAHGAALSQLLFLRQRIGDWHDLPVLRKQFREGVARNAP
                     LLSPFVLLSQPSTRSEQRRCAENWTAQVAPPVTVPGPMMLNSGRLRIGYLSADFHTHA
                     TAFLAAGLFEQHDRKRFEIVAYSTGHDDRSAMRARLAAGFDRFVDAAGWPALRLATAI
                     RNDAIDILVDLKGHTENAPPSVLAMRPAPIQVHYLGYPGTLGGNLADYLIADAIVTPT
                     QDAGDYAETLALLPGSYQVNDRARPIAEAPPRSELGLPRNGVVLCCFNSGYKFNPEVF
                     DAWAQILAQVPEAVLWLLARRGEERWVSNLRREMVRRGLDARRLIFATARPNPEYLAL
                     YRAADLFIDTWPYNAHTTASDALWAGCPLVTLRGETFASRVAASVLHAVGLPELVCDD
                     VKRYVAKIVMLARDADARGKLRAHLEGPGRASALFDTAATTRAIEAAYLAMADQYRRQ
                     VREPISVRAEDRAGATP"
     gene            complement(8510..9226)
                     /locus_tag="VFO53_04670"
     CDS             complement(8510..9226)
                     /locus_tag="VFO53_04670"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HET8875602.1"
                     /translation="MRYELYYWPGIQGRGEFVRLALEEAAAHYVDVAREHGGEDAMMT
                     LLDGERVARAPFAPPFLKAGRHLIGQTANILLFLGPRHGLSPGDEAGRLWTHQLQLTI
                     ADLVGEVHDTHHPIASSLYYEDQRREARKRAADLRTRRLPKFLGYFERILGRNARTAG
                     YLAGRRLTYADLSMFQVMAGLRYAFPNAMATLEPRCPALVRLHDRVRERPRIAAYLAS
                     PRRLPFNEEGIFRHYRELDG"
     gene            9396..9989
                     /locus_tag="VFO53_04675"
     CDS             9396..9989
                     /locus_tag="VFO53_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET8875603.1"
                     /translation="MSNDASTNALDLLKRDHQEVDKLFKQFDEIKEDAEASAKEELVT
                     QICDALTVHATIEEAIFYPAARQALPQQQGQDLLDEAAVEHQTLKDIIGRLEMAPTSD
                     PLYDAGVKVLSEYVKHHVREEETELFPKIRSSDMDLQAIGQQLEERKQQLEGQAARSS
                     RNGSRGRRSARSGTPMEGEQRARGQEGANEQRSRSTH"
BASE COUNT         1660 a         3430 c         3481 g         1631 t
ORIGIN      
        1 gaacgcatcg agatccgcgg gttcggcagc ttcggcctga actatcggcc gccgcgaacg
       61 gggcgcaacc ccaaatcggg cgagaaggtg caggtgcccg agaagtacgt gccccacttc
      121 aaggcgggga aggaactgcg ggaacgggtc gacttcaacc tcgtgcagga ttagcttcct
      181 tgatcaattt cctgcaggcg gccacgatcg attcgcgggc cgcctttctt ttggccggcc
      241 tgctgagatg agcctcttgc gctggatttt catcgcgttc gtgttcatcg cgctgctgtt
      301 catctcgctg cagaacgccg atccggtcac gttgcgcctg ttctcgctgt acgcgtggca
      361 ggcaccgctc gtgttcgtga tcctgctcgc gttcgcgacc ggtgtcgcgc tcggcctgct
      421 cgcgagcgcg atcaaggtcg tgcgtctcaa gcgccagctt gcgcggctgc gccgcgagca
      481 ccatcgcgcg cagtccgcgc tgcctgcggc acggggtccc gcgcccggca cggcggccgc
      541 ttccggcccc ggattcgagc cgccgtccac cgggttctga ccggcgcatg gatttcgagc
      601 tgtggtggct cctgccgatc ccggcgctgt tcttcgcgct cggctggatc gccgcgcgca
      661 tcgacatcaa gcatcttctg cacgagtcgc gtgcgctgcc gctgtcgtac ttccgcggcc
      721 tcaattttct gctgaacgag caacccgaca aggccatcga gtcgttcatc gaagtggtca
      781 aggtcgatcc gcagacgatc gacctgcact tcgcgctcgg gtcgctgttt cgccggcagg
      841 gcgagacgga gcgtgcgata cgcatgcatc agaatctgct cgaccggccc acgctgccgg
      901 cggacaagcg cacgatggcg acctacgagc tcgcgcagga cttccatcgc gccggactcc
      961 tcgatcgcgc cgagaatctt ttccagcgcc tcgacggcac gccgttcgag cattcggcgc
     1021 tgtcgcatct gatcgagatc tacgagacgg aaaaggactg gccgaaggcg atcaccgcga
     1081 tgcagcggat ggaggtgctc gcgaagcagc cgtactacaa ggagatcgcc gaataccatt
     1141 gcgagcttgc gcaggcggcg ctgctcaagt ccgatacgcc gacggcgagg gcggagatcg
     1201 agcgcgcgtt ggcggcgcac cgcggctgcg cacgcgcgaa cctgatcgcc ggcgacgccg
     1261 aggcgcaatc gggcgacttt cgcgcggcgg tcgaggcctg gcagcgcatc gagtcgcaga
     1321 atcccgcctt cctggccctc gccgccgatc gcttcgccga cgcgtttcgg cggctgcgcg
     1381 atctgccgac cggcatccgg ctgctgcgca actatcaggc gcagtattcg tcgctcgacc
     1441 tcctgaatgc gctcttcacg ctcacgctcg agcaggaagg cgccgatgcg gccgccacgc
     1501 tgatcaagga cgagctcgcg cgcaatccga cgctgatcgg cctcgatcgc ctgctggaag
     1561 cgcagcttct gacgtcgccc gccgatcggc ggcacgatct cgagctcgtc aagggactgg
     1621 tcggccagca catcaagcgg ctcggcctct acaagtgcga caactgcggc tttcgcgcgc
     1681 ggcaatacta ttggcgctgt cccggctgcc agaagtggga gacgtattcg ccgaagcgca
     1741 ccgaagcgcc ggacgggtac acgtaacgta aagcggcttc actgatgctc aggaccctca
     1801 catgactcgt gccaacccgc gcgtgctcgt tgcgctcgat ttcgccaacc ccatgcgcgc
     1861 gctcgcgctc gccgatcgcc tcgatcccgc cgaatgcggc gtcaaggtag gcaaggagct
     1921 tttcgtcgcc gcggggcccg agccggtgcg ctggctcgtc gcgcacggct tcaacgtctt
     1981 tctcgatctc aagttccacg atattccgaa cacggtcgcg cacgcgtgcg ccgcggcgac
     2041 gcgccttggc gtctggatgg tgaacgtgca cgcggcgggc ggcagcgcga tgctgcgcgc
     2101 ggcgcgtgaa gcggttgccg cgaccgcggg cgagacgggg attgcgcggc cgctgctgat
     2161 cgcggtcacc gtcctcacga gcctcgacgc ggcggcgctc ggcgaaaccg gcgtcgacgc
     2221 cgagccgggc gatcaggcgc tgcgcctcgc agcactcgcg caagcatgcg gcctcgacgg
     2281 cgtcgtgtgc tcggcccgcg aagcggctgc gatgcgcgcg agattcgggc cggacttcgc
     2341 gctggtgacg cccggcatac gtccgcgcgg cagccccggc gacgatcagg cgcgcatcgt
     2401 gacgcccgaa gaggcggtgc gcaacggcgc gagctacctc gtcgtcggcc ggccgatcac
     2461 cggtgccgcc gatccgccgg cagcgcttgc atccatcaac cgcgcgctgg gaatcggcgc
     2521 atgaagatca cgatgatcgg cacggggtac gtggggctcg tgaccggcac gtgcttcgcg
     2581 gaagtcggca acgacgtgct gtgcctcgac gtcgatgcgc gcaagatcga catgctggag
     2641 agcggcggcg tgccgatcca cgagccgggc ctcgagccga tgatccggcg caacgtcgcg
     2701 gccgggcgtc tgcgcttcac gaccgacgtg gatgctgccg tcggtcatgc gagcgtgcag
     2761 ttcatcgccg tcggcacgcc acccgacgaa gatggcagcg ccgacatgca atacgtgctg
     2821 caggcggcgc gcaacatcgg gcgacgcatg acgcaatgga cgctcgttgt cgacaagtcg
     2881 accgtgccgg ttggcaccgg cgatcgcgtc cgggcggtca tcgccgagga gctcgcagcg
     2941 cgcgggctcg atgttcccta cgcggtcgcc tcgaaccccg aatttttgaa ggagggcgcg
     3001 gcggtcgagg atttcatgaa gcccgatcgc gtcgtgatcg gtgccgacaa cgagcgcgcg
     3061 atcgccatcc tgcgcaacat ctacgcgccg ttccagcgca atcgcgaacg gctgctcgtg
     3121 atggacgtgc gctccgccga gctcaccaaa tacgccgcga acgcgatgct ggcaacgcgg
     3181 atctcgttca tgaacgagct cgccaatctc gccgaccggc tcggcgccga catcgaatcg
     3241 gtgcgccagg gcatcggctc ggacccgcgc atcggctggc atttcctcta tccgggtgtc
     3301 ggctatggcg gcagctgctt cccgaaggac gtgcgggcgc tgcagcacac ggcggcgcgc
     3361 gcgggcgcgc cgctcaaggt gctgtccgcc gtcgaggcgg cgaacgatgc gcagaagcag
     3421 gtgctcgtcg acaagatcgt cgcgcgcttc gggaacgacc tgtcgcagcg gcgcttcgcc
     3481 gtgtggggtc tcgcgttcaa gcccaacacc gacgacatgc gcgaggcgcc gtcgcgggtc
     3541 gtcatcgcag cgctgacgcg gcgcggcgcg acgatctgcg cgtacgatcc ggtggcgatg
     3601 gatgaagcac ggcgccttct cggcgacatg ccgcgcgtcg acttcgcgac cagtccgctc
     3661 gctgcgtgcg acggcgccga tgcgctcgtc gtcgtcaccg aatggaagga gtttcgcagt
     3721 cccgacttcg acgggttgca tcggcgcatg aagcagccgc tgatcttcga cggccgcaac
     3781 ctgtacgagc ccgacgtggt acgcgccgcc gggttcgagc acgtggcgat cgggcggcga
     3841 tgagcgcggc gggcgaggcc gccgagcgcg catcctccca ttggtcggac gcgccccgaa
     3901 gggcacggcg ccgcgcgagt cgcgcgagac cgacacgccg ctgtccgacc tgcgccggtg
     3961 gaacaaggag gtcgaggcga tgagcgcgca tttttccgcc tggcgcacgc gcgtggcggg
     4021 cgcgcaggtg ctgatcgtcg gcgacgtgat gctcgaccgc tactggttcg gcgacgtcga
     4081 gcgcatctcg cccgaggcac ccgtgccggt ggtgaagatc gcgcgctccg aagagcgccc
     4141 gggcggcgct gcaaacgtcg cgcgcaacat cgccgcactc ggcgcacgcg cgatgctgct
     4201 ctccgccacc ggtcgcgacg aggcggccga cacgcttctg cgccttctcg acagcgaccg
     4261 gatcgatgtg tcgttcctgc gcgacgaggc gctgaagacc acggtcaagc tgcgggtcat
     4321 cggccgccag cagcaattgc tgcgcatcga cttcgaaacg gcgccgccgc acgagttgct
     4381 cgcagcgcat ctggccgagt tcgaccatct gctcggcagc gccgatgcgc tcgtgctgtc
     4441 cgattacggc aagggcggcc tcgcgcacat tgccacgatg atcgagcgcg gccgcgcggc
     4501 cggcaagccg gtgctcgtcg atccgaaggg cgacgactgg gagcgctatc ggggcgcgac
     4561 gttgatcacg cccaaccgca gcgaattccg gcaggtggcc gggcgtgcac gcgacgagga
     4621 cgacctcgtg cgccgcgcgc agacgctgcg ccgcgatctc gatctcgacg cgttgctcct
     4681 cacgcgctcg gaggagggca tgtcgctctt ctccgacgcg ggcgcgctca cgatcccggc
     4741 gcaggcacgc gaagtcttcg atgtgtccgg tgcgggcgat accgtgatcg ccacgctcgg
     4801 cacgctgctc gccgccggcg cgccgatggc cgacgccatg gccatcgcca acgaagccgc
     4861 ggggatcgtc gtcggcaagc tcggcaccgc ggtcgccgag cccgacgagc tgcgttgatg
     4921 ccagatcggg gcacgaaatg acgtcgcccg atcgctgacc gtggccaagc cgaagacgct
     4981 ctacagttgc accgagtgcg gtggccaggc gccgaaatgg cagggacagt gcccgcagtg
     5041 cggtgcatgg aacacgctcg tcgagacgct tgcagcgccc gccgccgcgc ggttcgaaag
     5101 cgtcgccggc gcacgctcca ccgtaacgca actttcgtcg gtcacgccgc gcgcgacgac
     5161 gcgcatcgca accggtctcg aggagttcga tcgcgtgctc ggcaacggcc tcgtggccgg
     5221 cggcgtgatc ctgctcggcg gcgatcccgg gatcggcaag tcgacgctgc tgctgcaggc
     5281 gggtgcggcg ctcggtgcgc tgcatcgaac gctgtacgtg accggcgagg aatcggccga
     5341 gcagattgcg ctccgcgcac agcgcctcgg cctcgtcaac gcgccgatcg agctgctcgc
     5401 cgaggtgcag ctcgaggcga tcgtcgccgc gatcaacgcc acggcacccg agatcgtcgt
     5461 catcgactcg atccagacga cctataccga aacgctcgcg tctgcgccgg ggagcgtggc
     5521 gcaagtgcgc gagtgcgcgg cgcagttgac gcggctcgcc aagcagcgcg gcgtcgtggt
     5581 cattttcgtc ggccacgtga cgaaggaggg cgcaatcgcc ggtccgcgcg tgctcgagca
     5641 catcgtcgac acggtgctct acttcgaggg cgatccgcac tcgagcttcc gcctcgtgcg
     5701 cgcgatcaag aaccgcttcg gcgctgcaaa cgagctcggc gtgttcgcca tgaccgagcg
     5761 cggtctgcgc ggcgttgcga atccttcggc actgttcctt tcgcagcacg cggagggcgt
     5821 cgccggttcc gccgtggtcg cgacactcga aggctcgcgg ccgctgctcg tcgaagtgca
     5881 ggcgctggtc gaccccgtgc agggcggcat gccgcggcgc ctcgcggtcg ggctcgatcc
     5941 gcagcggctc gcgctgctgc tcgcggtcct gcaccgtcac ggcggcgtcg agacgagtgg
     6001 tttcgacgtg ttcgtcaacg cggtcggcgg ggtgcgcatc ctcgagccgg ccgccgatct
     6061 cgccgtgatg cttgcggttt gttcttcgtt gagaaacagc gcactgccga agaaatctct
     6121 tattttcggt gaagtcggcc tggcaggcga agtccggccg gtgcagcgcg gtcaggagcg
     6181 aattcgcgag gcagcgaagc tgggcttcga aacctttgtc atacctaacc tgaataaacc
     6241 taagcaacgc attgataatg ttaatatcat cggcgtcgat cgcgtcgacg aagcgattgg
     6301 ctggctgcga cggtgagacg caaaagccgg gtcacggcgt cgcgcccgca cggtcctccg
     6361 cgcgcacgct gatcggctcg cgcacctgcc tgcggtactg atcggccatt gcgaggtacg
     6421 ccgcctcgat ggctcgggtc gtggcggcgg tatcgaacaa tgcgctcgcg cgcccgggac
     6481 cttcgaggtg cgcgcgcagc ttgccgcgcg cgtcggcgtc gcgcgccagc atcacgatct
     6541 ttgcgacgta gcgcttgacg tcgtcgcaga cgagttccgg cagtcccacg gcatgcagca
     6601 cgcttgccgc gacgcgcgac gcgaacgttt caccgcgaag cgtcaccagc ggacagccgg
     6661 cccatagcgc gtcgcttgcc gtcgtgtgcg cgttgtacgg ccaggtatcg atgaacaggt
     6721 cggccgcgcg gtagagcgcc aaatactcgg gattgggacg cgccgtcgcg aatatcaggc
     6781 gccgcgcgtc gagcccgcgg cgcaccatct cccggcgcag gttgctcacc cagcgctcct
     6841 cgccccgtcg cgcgagcagc cacagtaccg cttcgggcac ttgcgcgaga atctgcgccc
     6901 aggcatcgaa cacttcggga ttgaacttgt agccgctgtt gaagcagcac agcaccacgc
     6961 cgtttcgcgg caatccgagc tcgctgcgcg gaggagcctc cgcaatcggg cgcgcacggt
     7021 cgttgacctg gtaactgccc gggaggagag cgagcgtttc cgcgtagtcg ccggcgtcct
     7081 gcgtcggcgt gacgatcgca tccgcgatga ggtaatcggc gaggtttccg ccgagcgtgc
     7141 cgggatagcc gaggtaatgc acctggatcg gcgcgggccg catggcgagc accgagggcg
     7201 gcgcgttttc cgtgtgtccc ttgaggtcga ccaggatgtc gatggcgtcg ttgcgaatcg
     7261 ccgttgcaag ccgcagcgcg ggccagcccg cagcgtcgac gaagcggtcg aacccggcgg
     7321 caagccgcgc gcgcatcgcg ctgcgatcgt cgtggccggt ggagtaagcg acgatctcga
     7381 agcgcttgcg atcgtgctgc tcgaagagtc ccgcggcgag aaaggcggtt gcatgcgtat
     7441 gaaaatcggc agacagatag ccgatccgca gccgcccgct gttcagcatc atcggaccgg
     7501 gaaccgtcac cggcggcgcc acctgcgccg tccagttctc tgcgcaacgg cgctgttcgc
     7561 tgcgcgtcga tggctgcgac agcaggacga acggcgacag cagcggcgcg ttgcgagcaa
     7621 cgccttcgcg gaactgcttt cgcaacacgg gcaggtcgtg ccagtcgccg atgcgctgcc
     7681 gcaggaaaag gagctgcgag agtgcggcac cgtgcgccgg gtcgagccga accaccgctt
     7741 cgtacgcctg catcgcgcgc tcgagctcgc cgtcgcgctc gagcaacccg gcgagctgga
     7801 agcgcagcgc cggcgactcc ggcttgcggg tgagggctgc atcgagcgtc gcgcgcgcct
     7861 tctttcgcat accggcggtg gcctcgagct gggccttgaa tcccgcgtac gcggcttcga
     7921 gcggcgcgag cgtgctcgcg cgcgacgatg cgtccatcgc ctcgagcagc cgcccctgcg
     7981 gctcgagcac gagcgccagg ttgtgccatc ccgcggcgtg ccgggcatcg acgtcgaccg
     8041 cgtttcgcgc cgcggcttcc gcctcggcga tgtggccgag cgccagctcg gcggcggcga
     8101 gattggcgag aatctcgggt gaacgcggcg cgatcttcgc cgcctcgtgc agtgcattca
     8161 ccgccgcctc ccgccgcccg gccgattcga gcacgagtgc caggttcgac caggcttcgg
     8221 acgacgccgg ctcgagctgc agcgatgcgc ggatgcgctc ggcggcttcc gggatcttgc
     8281 cggcctgata cagcaccacc cccgagtaat gcagcgccgg cgcgttcttc gggtcggcgg
     8341 ccaggaccgc gtcgtagcgc atgaacgctt cgcggagctt tccttcctga tgcaagcggt
     8401 aggcgcgatc gaattcggag gtgatgctca tcgtgtagcg gggcgtcgat gacgcgacgg
     8461 cgaaaatcgt ttgcgtacga gggcgagatt accatcgccc gaagcgcggt cagccatcga
     8521 gctcgcgata gtgacggaag atcccttcct cgttgaaggg cagccgtcgc ggcgatgcaa
     8581 ggtacgcggc gatgcgcggg cgctcgcgca cgcggtcgtg cagccgcacc agcgccgggc
     8641 agcgcggttc gagcgtcgcc atcgcgttcg gaaacgcgta gcgcaatcct gccatcacct
     8701 ggaacatgga cagatccgcg tacgtcaggc ggcgcccggc gaggtagccg gcagtgcgcg
     8761 cattgcggcc gagaatgcgc tcgaagtagc cgagaaactt cggcaggcgg cgcgtgcgca
     8821 aatcggccgc gcgcttccgc gcttcgcgcc gctggtcttc gtaatagagg ctgctggcga
     8881 tcgggtggtg cgtgtcgtgc acctcgccga cgagatccgc gatcgtcagt tgcagctgat
     8941 gcgtccacag ccgtcccgcc tcgtcgccgg gtgacagtcc gtgacgcggg ccgagaaaga
     9001 gcaggatgtt tgccgtctgg ccgatcaggt ggcggccggc cttcaggaat ggaggcgcaa
     9061 agggcgcgcg cgccacgcgc tcgccgtcga gcaacgtcat cattgcgtcc tcgccgccat
     9121 gctcgcgtgc gacgtcgacg tagtgcgcag ccgcctcctc gagcgcgagg cggacgaact
     9181 caccgcgacc ctggatcccc ggccagtagt acagctcgta gcgcatgtcg ttcctcccga
     9241 agcaagcagc gattctagcg gccagcccgc acgcgtgatg gccgccgtcg tccacgtcct
     9301 acacacgttg cagaagcggc cggacaggag acgcactcgg tacgccgtac attcgaatgc
     9361 gcctcaacgc ccccgggaga tctgcaatgt tcgcaatgag caacgacgcc agcaccaatg
     9421 cactggacct tctgaagcgc gaccaccagg aggtcgacaa gctgttcaag caattcgacg
     9481 aaatcaagga ggatgccgag gcctccgcga aggaggagct cgtcacgcag atctgcgacg
     9541 cgctgaccgt gcacgccacg atcgaggaag ccatcttcta cccggccgca cgccaggcgc
     9601 tgccgcagca gcagggacag gacctgctgg acgaggccgc cgtcgagcac cagacattga
     9661 aggacatcat cggccggctg gagatggcgc caaccagcga tccgctgtac gacgccggtg
     9721 tcaaggtgct gtccgagtac gtcaagcacc atgtgcgcga ggaagagacc gagctgttcc
     9781 cgaagatcag gtcgagcgac atggacctgc aggcgatcgg acaacagctc gaagagcgca
     9841 agcagcagct cgaaggacag gccgcgcgat cctcgcgcaa cggctcgcgc ggccgcaggt
     9901 ccgcgcggag cggaacgccg atggaaggcg agcaacgcgc gcgtggtcag gaaggcgcga
     9961 acgagcagcg gtcccgcagc acgcactgac gccgcgcgaa gcgcagtctc caggcagagc
    10021 cacgaaaccg ctgtggctct gttgcatctt gcccgggcgg cccgaactcg atgcatgccg
    10081 tacttcgtat aattgttatt atgtcaaatg tcacaacgga acaccgaagc gcacgcaacg
    10141 acccctttgt ctcgtgcgcc cgcccggttt acgtactccg gaatttggtg ataggtgttt
    10201 at
//
LOCUS       DASSVX010000103         5355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_536969, whole genome shotgun sequence.
ACCESSION   DASSVX010000103 DASSVX010000000
VERSION     DASSVX010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536969"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..749)
                     /locus_tag="VFO53_04680"
     CDS             complement(6..749)
                     /locus_tag="VFO53_04680"
                     /inference="COORDINATES: protein motif:HMM:NF039990.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HET8875604.1"
                     /translation="MYVPSLPETLDFSGPVGQIFDRQAQLRWTQAFDAGRTLGAGQWS
                     VALENPESVVTVPGGASFRADDDHVPDLTGQVVFDTSRGKFAVSAIVREIRADSRTPA
                     VVDRRTGGAVSVAGVMPALGRDDLRFVATAGNAIGRYSDGFFPDGVVDAAGRLALPRQ
                     WGWYAGYRHFWRQDLRSSLVLSGASETNPAGTPGSTNRNTHSLHANLIWSPIANADLG
                     VEYIHADRETQDGLNGRLNRFQASAKYVF"
     gene            complement(861..1175)
                     /locus_tag="VFO53_04685"
     CDS             complement(861..1175)
                     /locus_tag="VFO53_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875605.1"
                     /translation="MFRRHLHSPVRLVAVATLALGASSAAFADDNGMNVLTGDSYAYF
                     NNLDYRAGHFNVARASQAQPQDPVATMPKREPARAERPIMLAAPPKGITLHSPFRDDT
                     GA"
     gene            complement(1492..1860)
                     /locus_tag="VFO53_04690"
     CDS             complement(1492..1860)
                     /locus_tag="VFO53_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HET8875606.1"
                     /translation="MRFALPYAVIRPIRGVAVALAGTAIVAVFAIHASAAPPAKSTEV
                     AITKFAFAPKEITIVPGTQIVWTNHDDTPHSVAANDKSFSSKGLDTDDRFEHVFASEG
                     DFNYICTVHPFMTGVVHVRK"
     gene            complement(1899..2846)
                     /locus_tag="VFO53_04695"
     CDS             complement(1899..2846)
                     /locus_tag="VFO53_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8875607.1"
                     /translation="MNIPESRLTRRQALSCLATWTGAAVVWTVSGGVPRALGATGKDA
                     PAAAGGSDLTFVQISDTHIGFRKDANPDVVGSLRRAIADINGLPQTPAFVVHTGDVSH
                     LSKPEEFGKAREVLQEIRVDRVHTIPGEHDTIDDGATGYLRYFDHDGKGKSWYSFDHA
                     GVHFVGLNNVLNFKLGTMAALGDDQIGWLKGDLAGVSHSTPIVVLGHIPLWTIWEPWG
                     WGTADSAQAMALLRPYGSVTVLNGHIHQVLQKVEGSVALHTARSLAYPLPTPGQAGIG
                     EPGPVTVPAGELGKLLGIRQVNVVRGRHDLALIDTPLDR"
     gene            complement(2965..3843)
                     /locus_tag="VFO53_04700"
     CDS             complement(2965..3843)
                     /locus_tag="VFO53_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HET8875608.1"
                     /translation="MLPRLAGIILPVFSIIALGWLNAWRVKPDMEVTNRISMNVLAPA
                     LVFSALASRNFDVVANRLLIVGSVGVVLGSGLLAWPVARMMHANMRTFVPPMMFNNCG
                     NMGLPLAVLAYGAAGFSPMVALFTISNLLQFTLGVWIIDHHARFGNLVRNPMVIATVV
                     GFGFALAHPPLPEWLSVAIKLVGDALIPMMLLSLGVRLYEASFADWRIGVVGGLVCPL
                     TGIVMALLLAPALDLDATQRGLLILFGSLPPAVLNFMVAEQFHQEPGKVASIVLIGNL
                     MAIAFVPLGVALALRA"
     gene            complement(3855..5186)
                     /gene="hslU"
                     /locus_tag="VFO53_04705"
     CDS             complement(3855..5186)
                     /gene="hslU"
                     /locus_tag="VFO53_04705"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HET8875609.1"
                     /translation="MPLPMMPEEIVSELDKHIVGQDAAKRAVAIALRNRWRRQQVPDP
                     LRQEITPKNILLIGPTGVGKTEIARRLARLADAPFVKVEATKFTEVGYVGRDVDTIVR
                     DLVEVSIKQTREAETRRVRDRAADAAEERVLDVLLPPAREVNFEDMKPTDSATRQKFR
                     KMLREGQLDDKDIDIEISVLPPQMDIMAPPGMEDLTSQIQGMFQQIGGGRRKVKRTKV
                     PDALKQLTEEEAGKLVNDDDIKTRALAAAEQNGIVFIDEIDKIAARTDTHGADVSRQG
                     VQRDLLPLVEGTTVTTKYGMIKTDHILFIASGAFHLSKPSDLIPELQGRLPIRVELAP
                     LSIEDFERILTSTDACLTRQYEALLGTEQVHLAFAADGIRRIAEIAHSVNERQENIGA
                     RRLYTVMERLLEDVSFHAARRTGETIAIDAAFVDERLAGIAGDDNLAQYIL"
BASE COUNT          880 a         1714 c         1847 g          914 t
ORIGIN      
        1 gacgatcaga agacgtattt cgccgacgcc tggaaccggt tcagacgacc gttcaggcca
       61 tcctgggtct cgcggtcggc atgaatatat tcgacgccga gatcggcgtt tgcaatcggg
      121 ctccagatca ggttggcgtg cagcgaatgc gtgttcctgt tggtgctgcc cggggtgccg
      181 gcggggttgg tctcgctcgc gcccgacagc acgaggctgg atcgcagatc ctgcctccag
      241 aagtgccggt atccggcata ccaaccccat tgccgtggta gcgccagccg gcccgcggcg
      301 tcgacgacgc catccgggaa gaaaccgtcc gagtaacgtc cgattgcatt gccggccgtc
      361 gcgacgaacc ggaggtcgtc ccggccgagc gcaggcatca cgccggcgac gctgaccgcg
      421 ccacccgtgc gccgatcgac gactgcgggc gtgcgggaat cggcgcggat ctcgcgcacg
      481 atggcggaca cggcgaactt gccgcgcgac gtgtcgaaga cgacctgtcc ggtaaggtcg
      541 ggaacgtgat cgtcgtcggc gcgaaagctt gcaccgcccg gaacggtcac caccgattcc
      601 ggattctcga gcgccaccga ccattgaccg gcgccgagcg tgcggccggc gtcgaacgct
      661 tgcgtccaac gcagttgcgc ctggcgatcg aagatctggc cgacgggccc actgaagtcg
      721 agcgtttcgg ggagcgacgg aacgtacatg aagttggtcg acgaagagca cgtacttttc
      781 cgaagcattg cggcgccgca tggcggtttc ctcctcgatg ggacggccgc ggccgtccgc
      841 aggttgatcg aagccggtgc ttacgcaccc gtgtcgtcgc gaaacggcga atgcagcgtg
      901 attcccttcg gcggtgccgc cagcatgatc gggcgctcgg ctcgcgccgg ctcccgcttc
      961 ggcatcgtcg cgacggggtc ctgcggttgc gcctgcgaag cgcgtgcaac gttgaagtga
     1021 cctgcgcggt agtcgaggtt gttgaagtac gcgtacgagt cgccggtcag cacgttcatg
     1081 ccgttgtcgt cggcgaaggc ggcgctcgac gcgccaaggg cgagtgtcgc aaccgcgacg
     1141 agtcgtaccg gggaatgcaa atgcctgcga aacatgttgg gctccttcgt tgggtgtcga
     1201 tgacgcgtga tcgcgtaccg tgcaagactg cgcaggcgag cgcgttattc gagctggaat
     1261 aacgagggcg gcgatccggt cgcgccgctt gcttgccacc gtggaacggc gcagatgatg
     1321 ccggcgcgcg tgatggcgcg cggcgcgtcg aaccggccgt tggcgggtcg tgcctgccgg
     1381 cccgcgatgg gcgggccggt acaccgtgct tgaagggatt gcgtctggcg cgggacgcgc
     1441 caggcgtttg caccgagggc gggtgccgcg cggtgaccgc gctgcgccgt gctacttgcg
     1501 cacgtggacg acgccggtca tgaacggatg cacggtgcag atgtaattga agtcgccttc
     1561 gctcgcgaag acatgctcga acctgtcgtc ggtgtcgagc cctttcgagc tgaagctctt
     1621 gtcgttcgcg gcgacgctat gcggcgtgtc gtcgtgattg gtccacacga tctgcgtgcc
     1681 cggcacgatc gtgatttcct tcggcgcgaa ggcgaacttc gtgatggcca cctccgtgct
     1741 cttcgcgggc ggcgccgcgc tcgcatggat tgcgaacacc gcgacgattg cagtgccggc
     1801 cagggcgacg gccacgccac ggatcggacg gatgacggca taaggcagcg cgaatctcat
     1861 ggcagctccc ggaaggaagg agaatcggaa aatgcgcatc agcgatcgag cggcgtgtcg
     1921 atcagcgcga gatcatggcg cccgcgcacg acgttcacct gtcgtatgcc gagcagcttg
     1981 ccgagctcgc ccgcgggcac cgtgaccggg cccggctcgc cgatgcccgc ctgtccgggt
     2041 gtgggcagcg gataggcgag cgacctcgcc gtatggagcg cgacgctgcc ctcgaccttc
     2101 tgcagcacct ggtggatgtg tccgttgagc acagtgaccg aaccgtacgg gcgcagcagc
     2161 gccatcgcct gcgccgaatc ggcggtgccc cagccccacg gttcccagat cgtccacagc
     2221 gggatgtggc caaggacgac gatcggcgtc gaatgcgata cgccggcgag atcccctttc
     2281 aaccatccga tctggtcgtc gccgagtgcc gccatcgtcc cgagcttgaa gttgagcacg
     2341 ttgttcaagc cgacgaagtg cacgccggcg tgatcgaagc tgtaccagga cttgcccttg
     2401 ccgtcgtggt cgaaatagcg cagatagccg gtcgcgccgt cgtcgatcgt gtcgtgctcg
     2461 cccgggatcg tatgcacgcg gtcgacgcga atttcctgca gcacctcgcg cgccttgccg
     2521 aattcctcgg gcttcgacaa gtggctcacg tcaccggtgt gcacgacgaa ggcgggcgtt
     2581 tgcggcaggc cgttgatgtc ggcgatcgca cgccgaaggc tgccgacgac gtcgggattg
     2641 gcgtccttgc gaaagccgat gtgcgtgtcg ctgatctgca cgaatgtgag gtcgctgccg
     2701 ccggccgccg ccggcgcatc cttgccggtc gcaccgagtg cgcggggcac gccccctgaa
     2761 acggtccaga cgacggcggc tcccgtccac gtggcgagac agctcaaggc ctggcggcgg
     2821 gtcaagcgcg attccgggat gttcatcgac gctccttgtc gtgacggcgg gattgcctgt
     2881 actcgtcata tcgacaagcg cgacgattta ttcccggcac acgatgcgga cgccgcgcac
     2941 ggcggccacg cgagcggacg ccggctaggc gcggagcgcg agtgcgacgc cgagcggaac
     3001 gaacgcgatc gccatcaggt tgccgatgag cacgatcgag gcgaccttgc ccggctcctg
     3061 gtgaaactgc tcggccacca tgaaattcag gaccgcgggc ggcaggctgc cgaacaggat
     3121 cagcaggccg cgctgcgtgg cgtcgaggtc cagtgcggga gcgaggagca gcgccatgac
     3181 gatgcccgta agcgggcaga cgagaccgcc gacgacgccg atgcgccagt cggcgaagct
     3241 cgcttcgtaa aggcgcacgc cgagcgacag cagcatcatc gggatcagcg cgtcgccgac
     3301 gagcttgatg gcgaccgaca gccactcggg cagcggcgga tgcgcgagcg cgaacccgaa
     3361 gcccacgacg gtcgcgatca ccatcgggtt acgtacgagg ttgccgaagc gtgcgtggtg
     3421 atcgatgatc cacacgccca gcgtgaactg cagcaggttc gagatcgtga aaagcgcgac
     3481 catcggcgag aaaccggcgg cgccgtaggc aagcacggcg aggggcagtc ccatgttgcc
     3541 gcagttgttg aacatcatcg gcggcacgaa cgtgcgcatg ttcgcgtgca tcatccgtgc
     3601 gaccggccac gcgagcagtc ccgagccgag cacgacgccg acgctgccga cgatcagcag
     3661 cctgttcgcc acgacgtcga agttcctgct ggcaagcgcc gagaagacga gcgccggcgc
     3721 gagcacgttc atgctgatgc ggttcgtcac ctccatgtcg ggcttgacgc gccacgcatt
     3781 gagccagccg agcgcgatga tcgagaacac cggcaggatg atgccggcga gacgggggag
     3841 cacgcccctc ggccctacag gatgtactgg gccaggttgt cgtcgccggc gatgccggcg
     3901 aggcgctcgt cgacgaacgc cgcgtcgatc gcgatcgtct cgccggtgcg ccgcgcggca
     3961 tggaacgaca catcctcgag cagccgttcc atcaccgtgt agaggcggcg ggcgccgatg
     4021 ttctcctgcc gctcgttcac cgagtgcgcg atctcggcga tgcggcggat gccgtccgcc
     4081 gcgaaggcga gatgcacctg ctcggtgccg agcagcgcct cgtactgccg cgtgagacac
     4141 gcatcggtgg acgtcaggat gcgctcgaaa tcctcgatgg acagcggtgc gagctcgacg
     4201 cggatcggca ggcgtccctg cagctcggga atcaggtccg acggcttcga caggtggaat
     4261 gcgcccgatg caatgaacag gatgtgatcg gtcttgatca ttccgtactt ggtcgtgacg
     4321 gtggtgcctt cgacgagcgg cagcaggtcg cgctgaacgc cctggcgcga gacgtcggca
     4381 ccgtgcgtgt cggtgcgcgc agcgatcttg tcgatctcgt cgatgaacac gatgccgttc
     4441 tgctcggcgg cggcaagcgc gcgcgtcttg atgtcgtcgt cgttgacgag cttcccggcc
     4501 tcctcctcgg tgagctgctt cagcgcatcg ggcaccttcg tgcgcttcac cttgcgccgc
     4561 ccgccgccga tctgctggaa cataccctgg atctgcgacg tcaggtcctc catgccgggc
     4621 ggcgccatga tgtccatctg cggcggcagc accgaaatct cgatgtcgat gtccttgtcg
     4681 tcgagctggc cctcgcgcag catcttgcgg aatttctgcc gggtcgccga gtcggtcggc
     4741 ttcatgtcct cgaagttcac ttcgcgcgcg ggcggcagca gcacgtcgag cacgcgctcc
     4801 tcggccgcat cggcggcgcg gtcgcgcacg cggcgcgttt ctgcttcgcg cgtctgcttg
     4861 atcgacacct cgacgagatc gcgcacgatc gtgtcgacat cgcgtcccac gtagccgact
     4921 tcggtgaatt tcgtcgcctc gaccttgacg aacggcgcgt cggcgaggcg cgcgagccgg
     4981 cgcgcgattt ccgtcttgcc gacgccggtg ggaccgatga gaagaatgtt cttcggcgtg
     5041 atttcctggc gcagcgggtc gggcacctgc tgccggcgcc agcggttgcg aagcgcgatg
     5101 gcaacggcgc gcttcgccgc atcctgcccg acgatgtgct tgtcgagctc ggacacgatt
     5161 tcctcgggca tcatcggcaa cggcatcgct atcctcaaat gaaacggccg ccgaaacgtc
     5221 ggcggccgca tcattttacc gcgcgagctt gcgcgggatg gtgcgcgcgt cttacggggc
     5281 gagcaattgc gcgcagttcc ccggctccga tggattcggc gagatggccc cgcccttcgg
     5341 gatgcattgg gaggg
//
LOCUS       DASSVX010000104         5228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1336840, whole genome shotgun sequence.
ACCESSION   DASSVX010000104 DASSVX010000000
VERSION     DASSVX010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336840"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /gene="groEL"
                     /locus_tag="VFO53_04710"
     CDS             <1..796
                     /gene="groEL"
                     /locus_tag="VFO53_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET8875610.1"
                     /translation="DRRKAMLEDIAILTGGKVIAEEVGLTLDKVTLADLGQAKRVEVA
                     KENTTIIDGAGAASDIEARVKQVRVQIEEATSDYDKEKLQERVAKLAGGVAVIKVGAA
                     TEVEMKEKKARVEDALHATRAAVEEGIVPGGGVAYLRARANVKGLKGDNGDQDAGIKI
                     VLRAVEEPLRQIVGNAGEEPSVVLNKVVEGKGNFGMNAATGEYGDLVEMGVLDPTKVA
                     RTALQNASSVASLMLTTDAMVAELPKDDKPAMPGGGGMGGMGDMDM"
     gene            1044..1634
                     /locus_tag="VFO53_04715"
     CDS             1044..1634
                     /locus_tag="VFO53_04715"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HET8875611.1"
                     /translation="MTSGLVFGFAVITLAAVIVAGLMRRSREAPAGPRSSLDGPTAMP
                     QWTEGETTRLLSSAEVPGLYLLRPRFLSKAESVVFLLLKAAFPRHEVFARIRLADVLQ
                     VKIGPQGMERLRAFRKIANQHVGFVICDRDMTIVAIVDAKEPDQVIDPRDQKLETIKQ
                     RCLQAAQVKYVCIYPPQLPRYRELREQILGPAGELV"
     gene            complement(1687..1947)
                     /locus_tag="VFO53_04720"
     CDS             complement(1687..1947)
                     /locus_tag="VFO53_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET8875612.1"
                     /translation="MGLLHIIGMIVVGFIVGWLAQAFYPGAQAMGFWATAAIGIIGSL
                     VGGLIGGFVWRSPDGKFHPAGFVLSIVGALVVLWLYLTFIAK"
     gene            2120..2902
                     /locus_tag="VFO53_04725"
     CDS             2120..2902
                     /locus_tag="VFO53_04725"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HET8875613.1"
                     /translation="MRLLLQLVVLFAALTGVEAHAASACHASSGTLTRPLIELYTSEG
                     CDSCPPAERWLSSRFGAAGTASQAIALAFHVDYWDRLGWVDRFANADYTARQYAMMHA
                     NGGTFVYTPQLLLQGRDFAAWRDADTSPIDAAARTNAAATIVVDVVPGERELVVHADA
                     RIVHDSGAHDAELFVAYADSGLVSQIGAGENRGLRLTHDHVVRTLRLAGRDSGAGHVD
                     ATLTLTRPIERGARPMLVAFVERASTGDVLQALALPLDACTR"
     gene            complement(2921..3904)
                     /locus_tag="VFO53_04730"
     CDS             complement(2921..3904)
                     /locus_tag="VFO53_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET8875614.1"
                     /translation="MHLVCALAACAFAATLAPVHAGSARAARPFDLEGHRGARGLAPE
                     NTLAAFRKAIAIGVTTIETDIAVTKDRVPVISHNPSLVPELVRDGTGKWLTTPGPSIH
                     SLTLDELRRYDIGRLDPDTKYARQFPEQRPSDGERFPTLREVLQLVAAAGKPVRLNIE
                     TKLTPDNAGDTVDAATFVRVILDEVRAAELVDRVTIQSFDWRSLIEVKRVAPDVPTSC
                     LTIESTGMNTMAQAPDGKSPWHAGLKDSDYRSVAALVAAAGCSTWSMFWRNLTPALVA
                     DAHASGLEVLPWTVDDPADMQRLIDMRVDGIITDYPDRLREVMKARGMPPP"
     gene            complement(3917..4249)
                     /locus_tag="VFO53_04735"
     CDS             complement(3917..4249)
                     /locus_tag="VFO53_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8875615.1"
                     /translation="MAIPHASPNEVIDVRPLGRPITEDDSQLLIRTAHLQVFRYALPA
                     GKSIQEHTAAGVMIVQCIEGAVAFTAQGRTQTLAAGDMLYLADEAPHSLAALADSSLL
                     VTILLHRT"
     gene            complement(4284..4634)
                     /locus_tag="VFO53_04740"
     CDS             complement(4284..4634)
                     /locus_tag="VFO53_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET8875616.1"
                     /translation="MDHSMYPDTTRELHEKRRELAPEAYAAFRTFSQKVFADGALPSK
                     TKQLIAVAVAHVTQCPYCIRGHTEAARRQGATEQEVMEAIWVAAEMRAGAAYAHANLA
                     IDTMNRMAAKQAGD"
     gene            complement(4649..5113)
                     /locus_tag="VFO53_04745"
     CDS             complement(4649..5113)
                     /locus_tag="VFO53_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET8875617.1"
                     /translation="MPSRIDFPKAAPGAYMAMRGLEAYVRQSGLEPGLLELVKTRASQ
                     LNGCAYCIDMHTKDARAHGETEQRLYALSAWRETPFFSERERAALAWTEAVTLVADAP
                     IDEDDYDDVREHFDEKSLADLTLAIVAINGWNRLSIAFATVPGSYRRPTPEE"
     gene            complement(5122..>5228)
                     /locus_tag="VFO53_04750"
     CDS             complement(5122..>5228)
                     /locus_tag="VFO53_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HET8875618.1"
                     /translation="VQRGDLIVLTIGEPMGKSGGTNTMKIVRVGDHRA"
BASE COUNT          845 a         1736 c         1782 g          865 t
ORIGIN      
        1 cgaccgccgc aaggccatgc tggaagacat cgccatcctg acgggcggca aggtcatcgc
       61 cgaggaagtc ggcctgacgc tggacaaggt cacgctggcc gacctcggcc aggccaagcg
      121 cgtcgaagtc gccaaggaga acaccacgat catcgacggc gccggcgcgg ccagcgacat
      181 cgaggcgcgc gtcaagcagg tgcgcgtgca gatcgaggaa gcgacctccg actacgacaa
      241 ggaaaagctg caggagcgcg tggcgaagct cgctggcggc gtggccgtga tcaaggtcgg
      301 cgccgcgacg gaagtcgaga tgaaggaaaa gaaggcgcgc gtcgaagatg cgctgcacgc
      361 gacccgtgcg gccgtcgagg aaggcatcgt cccgggcggc ggcgtcgcgt accttcgtgc
      421 gcgtgcgaac gtcaagggcc tcaagggcga caacggcgac caggacgccg gcatcaagat
      481 cgtgcttcgc gcggtcgagg agccgctgcg ccagatcgtc ggcaacgccg gtgaagagcc
      541 gtcggtcgtg ttgaacaagg tcgtcgaggg caagggcaat ttcggcatga acgccgcgac
      601 cggtgagtac ggcgatctcg tcgagatggg cgtgctcgat ccgaccaagg tcgcgcgcac
      661 cgcgctgcaa aacgcctcgt cggtggcgag cctcatgctc accaccgatg cgatggtggc
      721 cgagctgccg aaggacgaca agccggcgat gcccggtggc ggcggcatgg gcggcatggg
      781 cgacatggac atgtagttgc ggtccacgcc gaaagcagcg tctgcagtac gaaagggccc
      841 gcgcaaaacg cgggcctttt caattccaag ttgcgttttt cgcaacgcgc tgcctgcgtt
      901 tgcgctgcgc gacaccggct gtcgccgcgt gctgaccgaa atgctttaga ctaggcgcgg
      961 ccgcgcaaat cggcacgatc tgcaatgttc acgcatcgat catccgccgt gctcgctcac
     1021 gtcatgccat tcatccggca tcgatgacct ccgggctcgt cttcggtttc gccgtcatca
     1081 cgctcgccgc ggtgatcgtc gcggggctga tgcgccgaag tcgtgaggca cccgcgggtc
     1141 cgcgatcgtc gctcgacggg ccgaccgcaa tgccgcagtg gaccgagggc gagaccacgc
     1201 ggctgctctc gtcggccgag gttccggggc tttacctgtt gcgcccccgc tttctttcca
     1261 aggccgagag cgtcgtattc ctgctgctga aggcggcctt tccccggcat gaggtgttcg
     1321 cgcgcattcg cctcgcagac gtcttgcagg tgaagatcgg gccgcagggc atggagcgcc
     1381 tgcgcgcgtt tcgcaagatc gccaaccagc acgtcggctt cgtcatctgc gatcgcgaca
     1441 tgacgatcgt cgccatcgtc gatgcgaagg agcccgatca ggtgatcgat ccgcgcgacc
     1501 agaagctcga gacgatcaag cagcgctgcc tgcaggcggc gcaggtcaaa tacgtctgca
     1561 tctacccgcc gcaactgccg cgctaccgcg agttgcgcga gcagattctc gggccggcgg
     1621 gcgagctcgt ctagccatcg caggtcccgc gcggcatcga agctgcccgc gcggacaccc
     1681 aacccgctac ttggcgatga acgtcaagta gagccagagg acgacgagcg cgccgacgat
     1741 cgacagcacg aagcctgccg ggtggaactt gccgtccggc gaccgccaga cgaagccgcc
     1801 gatcaggccg ccgaccagcg atccgatgat tccgatggcg gcggttgccc agaatcccat
     1861 cgcctgcgcg cccgggtaga acgcctgcgc gagccagccg acgatgaagc caacgacgat
     1921 catcccgatg atgtgcagca ggcccacgtg aacctcctcg ttgacggggc gcgccgaaac
     1981 cgcgcgccgg ggacggcagc gtagcatacc ggccgacggg tgcgcgtaag ccggcccgcg
     2041 ggttcgacga ctgtccaata tcgccgcgat ctgcgacacc cgcggcagtg gcggcggcgt
     2101 cgagccaggg agaatcgcga tgcgtttgct tttgcaactg gtcgtcttgt ttgcggcatt
     2161 gaccggcgtg gaggcccacg ccgcatcggc gtgccatgcc tcgagcggca cgctgacgcg
     2221 cccgctgatc gagctttata cgtccgaggg ctgcgatagc tgtccgcctg ccgagcgctg
     2281 gctatcgtcg cggttcggcg cggccggcac cgcctcgcag gcgatcgcgc tcgccttcca
     2341 tgtcgactac tgggaccgtc tcggctgggt cgaccgcttc gccaacgccg actacacggc
     2401 gcgccagtac gcgatgatgc atgccaacgg tggcacgttc gtctatacgc cgcagctgct
     2461 gctgcagggc cgcgattttg ccgcctggcg cgatgcggac acgtcgccga tcgacgcggc
     2521 cgcgcgcacc aacgccgccg cgacgatcgt cgtggacgtc gtccccggcg agcgcgagct
     2581 cgtcgtccat gcggacgcgc gcatcgtgca tgacagcggc gcgcacgatg ccgagctttt
     2641 cgtcgcctac gccgacagcg gactcgtctc gcagatcggt gcgggcgaga atcgcggcct
     2701 gcgcctgaca cacgaccacg tcgtgcgcac attgcgcctc gcgggacgcg acagcggcgc
     2761 gggccatgtc gacgcaacgc tgacgctgac gaggccgatc gagcgcggtg cgcgaccgat
     2821 gctcgttgcg ttcgtcgagc gtgcatcgac cggcgacgtg ttgcaggcgc tcgcgctgcc
     2881 gctcgacgcc tgcacccgct gaagctactt gtgctcgccc tcaaggcggc ggcatgccgc
     2941 gcgccttcat gacctcacgc agccggtcgg gatagtcggt gattatgccg tcgacgcgca
     3001 tgtcgatcag ccgctgcatg tccgcggggt cgtccaccgt ccatggcagc acctcgaggc
     3061 cgcttgcatg cgcgtcggcc acgagtgcgg gcgtcaggtt gcgccagaac atcgaccagg
     3121 tgctgcaacc ggccgcggcg accagtgccg ccaccgagcg gtaatcgctg tccttcaacc
     3181 cggcatgcca cggcgatttc ccgtccggcg cctgcgccat cgtattcatg cccgtcgact
     3241 cgatcgtcag gcacgacgtc ggcacgtccg gtgcaacgcg cttcacttcg atgagcgatc
     3301 gccagtcgaa cgactggatc gtcacccggt cgacgagctc cgccgcccgg acctcgtcga
     3361 ggatcacgcg cacgaacgtg gccgcatcga cggtatcgcc ggcgttgtcg ggcgtgagct
     3421 tggtttcgat gttgagccgt accggcttcc ccgcggccgc aacgagctgc agcacttcac
     3481 gcagcgttgg aaagcgctcg ccgtcgctcg gacgttgttc gggaaactgg cgggcgtact
     3541 tcgtgtccgg atcgagccgg ccgatgtcgt agcgccgcaa ttcgtcgagc gtcagcgaat
     3601 gaatcgacgg tcccggcgtc gtcagccact tgcctgtgcc gtcgcggacg agctccggga
     3661 cgagactcgg attgtggctg atgaccggaa cccgatcctt cgtgacggcg atgtcggttt
     3721 cgatggtcgt cacgccgatt gcgatcgcct tgcgaaaggc cgcgagcgtg ttctcgggtg
     3781 cgaggccgcg cgcgccgcga tgaccttcga ggtcgaaagg acgggccgcc ctcgcgctgc
     3841 cggcatgcac gggcgcgagg gtcgccgcga acgcgcatgc ggcgagcgcg cagacgaggt
     3901 gcaacgcgtg acgcacctac gtacggtgca acaggatcgt gacgagcagc gacgaatcgg
     3961 cgagggccgc gagcgaatgc ggcgcctcgt cggcaaggta cagcatgtcg ccggcggcga
     4021 gcgtctgcgt gcgtccctgc gccgtgaacg cgacggcgcc ttcgatgcat tgcacgatca
     4081 tcacgccggc ggccgtgtgc tcctggatcg acttgccggc gggcagcgca tagcggaaca
     4141 cctggagatg cgcggtccgg atcaacagct gcgagtcgtc ctcggtgatc ggccggccca
     4201 gcgggcgcac gtcgatcact tcgttcgggg atgcgtgcgg gatcgccatc gtcagtcctc
     4261 cggtaacgcc cgttgacagc cgctcagtcg cccgcttgct tcgctgccat ccggttcatc
     4321 gtgtcgatcg ccagattggc gtgcgcgtaa gcggcgccgg cgcgcatttc cgcggcgacc
     4381 cagatcgcct ccatcacttc ctgctcggtg gcgccctgcc tgcgcgccgc ctcggtgtgc
     4441 ccgcgaatgc agtacgggca ctgcgtgacg tgcgcgacgg cgacggcgat cagctgcttc
     4501 gtcttcgacg gcagcgcacc atcggcgaac accttctggc tgaacgtgcg aaatgccgcg
     4561 taagcctcgg gagcgagctc gcgccgcttc tcgtgcagct cgcgggtcgt gtcgggatac
     4621 atcgaatggt ccatgtgcac ctcccttgtc actcttccgg cgtcggccgc cggtagctgc
     4681 cgggcacggt ggcgaaggct atcgacaacc ggttccagcc gttgatggca acgatggcga
     4741 gcgtcaggtc ggcgagcgat ttctcgtcga agtgctcgcg cacgtcgtcg tagtcgtctt
     4801 cgtcgatcgg cgcatcggca acgagcgtga cggcttcggt ccatgcgagt gccgcgcgct
     4861 cgcgctcgga gaagaacggt gtctctcgcc aggccgacag cgcgtagagg cgctgctcgg
     4921 tttcgccatg cgcgcgcgcg tccttcgtgt gcatgtcgat gcagtatgcg cagccgttga
     4981 gctgcgaggc gcgggtcttc acgagctcga gaaggcccgg ctcgagtccg gactggcgca
     5041 cgtaggcctc gaggccgcgc atcgccatgt aggcgccggg tgcggctttc gggaagtcga
     5101 tgcgggaggg catgggtagc gtcaggcgcg gtggtcaccg acccgcacga ttttcatggt
     5161 gttcgtgccg cccgactttc ccatcggctc gccgatcgtc agcacgatga ggtcaccgcg
     5221 ctgcacgt
//
LOCUS       DASSVX010000105        13481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1403733, whole genome shotgun sequence.
ACCESSION   DASSVX010000105 DASSVX010000000
VERSION     DASSVX010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 13481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13481
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403733"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3555)
                     /locus_tag="VFO53_04755"
     CDS             complement(<1..3555)
                     /locus_tag="VFO53_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET8875619.1"
                     /translation="MEFDLGPLTWVQGEIDDALTRGTGALAAFRANPADTASLKHARS
                     HIHQAAGAIQMVGLDGVVAFTGEIERQLGRMEELAPDALPHAVAVVDHACNRLRVFLD
                     EVTNGAPPIPLALAVEYEAMRKARGEEAAAPTDLFYPDLNVRPPRGTPAEIVPAARLS
                     SHLLKERRQYQRGLLEWLRGNASGAAMMREAVAAIESVTAQSNLRAFWWTAGALLEAL
                     ETNALAPSFGAKQLVARIDMQIRRVTEGSAKVADRLRREVLYYIAIAAPAAPQIEAVQ
                     RAYQLAALLPTPEALDADIVRVKPLLREAREQLASAKDAWLKAASGRAESLPKLTQLL
                     ASAHAKAQEIGHPALAKLTAALAERLAAMPPQGASEPIAMEFATALLLAESAFENFGN
                     LSPDFASQVDAMLARLAAVHTGEAPSHGAPALDEMSRRAQERVLLGQVMREVQANLRH
                     MEQVLDAFFRDHTRRLELGGLTRDSQQIRGALRMLELDDADRLLALCQAQIESYADPA
                     TAVDEDGLELLAESLSGLGFYIDAVLHQRPERERIIAPLIAKREGVAPRAEVVEPQSL
                     EDSVAELRAALPRLLADVRDAPADASARAELRGKLVSLRDDADLIGDAELAAQVKAAI
                     REIDGGGAEAALAASVDLIVESGATPAPALSEETQRLLATDARALDAEILDIYLTEAG
                     EVLDAVDANLRVLEHSPADRDALVTVRRQFHTLKGSGRMVGLTELGELAWGVERIHNR
                     LLEEDRRVTPAFLALIGVAASSFRQWVRELRESGRFATDASPLLSAMRAVEAEWPGAS
                     SAVLSLPPVAPAKPSPVAAEHPIGPVSPPRIEFPADGVETFTPFAGETGGHIEFPAPI
                     DEAARLEFAAPGQTLDAEFPLAADEVPSDDTRSSHELPQLPDLELVTFPELGAPIEGG
                     AAPRVTVLEESMGETIDVGLPLDLRDAGKPVLRVVADNTSTLPADPRARTSSAHTPDL
                     TLLSDAPRAPAAAPESDELTVGDVRLSSSLWRILCDEADQHVALLQHEVSVLQFDPDH
                     WPIATMVRASHTLCGIHRTGGIALIATTAQALEQALLALGEHGAPFPGIAQPVLARAT
                     AGLAHFVGRVKARDGFTPGDEREAFEIATELDELRQEALANLPPAEPLIPAEEGSDRL
                     ELVADEHDVPVESIEADARGVEVAPETTVEAVAADAVAE"
     gene            complement(3611..5815)
                     /locus_tag="VFO53_04760"
     CDS             complement(3611..5815)
                     /locus_tag="VFO53_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET8875620.1"
                     /translation="MALKMTKLFGGEPHAAENSDFDVPTTQVRMGITSPVGFDPVTSI
                     TVAEQLRAASNNAPPPSKLPLIGGLPVVRQFQILGVLTTAFVALAVSMVYADNRAASQ
                     QALHSSAATEMQMLSQRLARGSALAAQGQAAAFAAIRDSRNRFKANLDALTGNGAFKG
                     VALDPTHDPAAAGTLGTIKGRFERIDAAAKRLTDNEASLTSLARGLAAINSGNSGLLE
                     LAQQAAQQIGQAGGSLREVDYANQLAVLSQRIAKNANTLASANEIDPEVAFLFGKDDA
                     AFRDVVNGLAKGSDALRLAPARSDDARATLAELARRFTDYESGVNAILSNMANLVVAK
                     QAARSINNEAEPLLDDTTTLAAQYENGAQAHALTLGLAIAFGLVALGCLALIAKVFLD
                     DARLRAQDSEHENKRNQEAILRLLNEMGNLADGDLTVQASVTEDVTGAIADSINFTIE
                     ELRALVRGINSATDQVTKATQETQAISNRLYEASQRQNREIQQASASVLTMADSINEV
                     ASNAAQSAKVAQTQLAAAEKGGSAVQNQISGMNDIRSQIQETSKRIKRLGESSLEIGE
                     IVELIADITEQTNVLALNAAIQAASAGEAGRGFTVVAEEVQRLAERSGEATKQIEAIV
                     KTIQADTQDAVAAMEKSTVGVVEGAKLSDAAGQALLEIQRVSRELAELIGRISAQTQR
                     QSASVTDVTRGMQGILRISEETTEGTKQTNVSIGQLTKLAAELRSSVAGFKV"
     gene            complement(5895..6416)
                     /locus_tag="VFO53_04765"
     CDS             complement(5895..6416)
                     /locus_tag="VFO53_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET8875621.1"
                     /translation="MSRAARLDLRSFQRELASRLASKTAAQVESSRLGIACGDKRWLI
                     RLGEAGEVVAVPPIVPVPLTKPWFLGLANIRGNLFSVVDFPAFLGGAPVSTAGISRLI
                     LLSTRSGGESVGIVVERVLGLRNLAQFTTDAVAEGAPAWQVAGWRDAEALAWQEIDLG
                     MLARDPAFLQVGA"
     gene            complement(6443..6808)
                     /locus_tag="VFO53_04770"
     CDS             complement(6443..6808)
                     /locus_tag="VFO53_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8875622.1"
                     /translation="MGIKRILVVDDSATERHMLKDLLTKAGYDVISSENGEDAIVKAR
                     TVKPDLILMDVVMPGLNGFQATRAISRDPETKSIPVIMCTSKSQETDKIWGLRQGAKD
                     YVVKPVDREVLLAKIASLG"
     gene            complement(6948..7358)
                     /locus_tag="VFO53_04775"
     CDS             complement(6948..7358)
                     /locus_tag="VFO53_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8875623.1"
                     /translation="MGNDTPGGSPLSGIKVMVIDDSNTIRRSAEIFLLQAGCTVILAE
                     DGFDALAKIADHQPDLVFVDIMMPRLDGYQTCALIKKNSRFHAMPVIMLSSKDGLFDR
                     ARGRMVGSDQYLTKPFTKESLLKAVAAHVRAVAA"
     gene            complement(7681..9195)
                     /gene="ilvA"
                     /locus_tag="VFO53_04780"
     CDS             complement(7681..9195)
                     /gene="ilvA"
                     /locus_tag="VFO53_04780"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HET8875624.1"
                     /translation="MPVDYLQKILTARVYDVAIETPLDVAPALSARLGNHVLLKREDQ
                     QPVFSFKLRGAYNKMAQMSAAERARGVIAASAGNHAQGVALAAQRLGCTATIVMPVTT
                     PRIKIAAVEARGACVILHGDSYSDAYDRAQALQQELGATFVHPYDDPDVIAGQGTIGM
                     EILRQCPGPLDAIFVAIGGGGLVSGIAAYVKRVRPDVAIIGVQPEDSDAMARSLAAGR
                     RVTLAHVGLFADGVAVKQVGKETFRLARKYVDDVVVVDTDAICAALKDVFEDTRAIVE
                     PAGALGVAGVKAWVQRTRVKGQTFAAIACGANMNFDRLRFVAERAELGEQREAILAVS
                     IPERPGSFREFCALLGRRSVTEFNYRYADPRVAHLFVGIEVANRRETEQLLAALKRAR
                     IEASDLSDNETAKLHVRHLVGGHAPAAEHEIVYRFEFPERPGALMRFLDSMSARWNIS
                     LFHYRNHGADYGRVLVGMQVPPRDSGAFRAFLDGLGYDYVEESANPAYRMFLGG"
     gene            complement(9358..9768)
                     /locus_tag="VFO53_04785"
     CDS             complement(9358..9768)
                     /locus_tag="VFO53_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET8875625.1"
                     /translation="MPDELRPLTPLGRKQAARMAHWLDERLPAGCRIIVSPALRAQQT
                     AQALDRKFESVPEIGTGASVAAVLATAGWPEARAPVLVVGHQPTLGRVASFLLAGDEI
                     DQAMHTGAVWWLSNRVHQEPAAVVLKVAIGPDFV"
     gene            complement(9842..10585)
                     /gene="dnaQ"
                     /locus_tag="VFO53_04790"
     CDS             complement(9842..10585)
                     /gene="dnaQ"
                     /locus_tag="VFO53_04790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HET8875626.1"
                     /translation="MRQLLLDTETTGLDPRQGHRIIEIAAVEVVNRRLSGRHLHFRLD
                     PDRDIDAAATEVHGLTRDDLRGKPRFADIAAEFVDFACGAEWIIHNAPFDVAFFDAEF
                     GLCGLPKSDAIYGRLIDTLALARDTFPGKRNNLDALCERFGVSNAQRTLHGALLDAQL
                     LAEVYLAMTRGQETLVIDMYAPPVPAGGLAIDGAAMRAGRYVVHRIVPDDAELALHRA
                     YLDALARECKSGCVWLALEAAASVAIAVA"
     gene            complement(10593..11507)
                     /locus_tag="VFO53_04795"
     CDS             complement(10593..11507)
                     /locus_tag="VFO53_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HET8875627.1"
                     /translation="MRFTIFQESRKGAREVNQDRIAYTYSRETLLLVIADGMGGHVGG
                     EIAAQIAVRLFIERFHQEAKPILRNPLKFMQETMLRAHAALGSYANQFSMLETPRTTC
                     VAVVVQGGHAYWTHVGDSRFYLLRQGRLIASTKDHSKVQYLVDQGLITPEEVLMHPDR
                     NKIFSCLGGMADPVIDLSRRTQLLDGDLLILCTDGLWSVYPHGEMATMLTSTPILATA
                     PQIMREAEKRGGDEGDNVSAIIVRWGPETLADEPTTTENLALGQFETQVDATVTLADR
                     AGGQRDLTDDEIERAIAEIQSTIQKYRK"
     gene            complement(11522..12460)
                     /locus_tag="VFO53_04800"
     CDS             complement(11522..12460)
                     /locus_tag="VFO53_04800"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET8875628.1"
                     /translation="MPVTNQALPGGYALLNYRIDRQISRGGFSIVYRAFDETGQPVAI
                     KEYLPSSLVLRTEGDIVRASSAENLTSFRYGMKCFFEEGRALAKINHPNVVRVLNFFR
                     ANETVYMVMRYERGRTLQQQIQMRQDQMSERFVRQVFMQLLNGLREVHTHKILHLDIK
                     PANIYLRTDGSPVLLDFGAARQTLTTNRPKLAPMYTPGFAAPEQYRDHDRLGPWTDVY
                     GTGASMFACLAAFAPQAADARVQEDHLVSAKKIWSGQYSDNLLEVIDWCLRLDPLERP
                     QSVFALQRAIRDIAPIKRKLTFFANLKRLLFSEIGV"
     gene            12634..>13481
                     /locus_tag="VFO53_04805"
     CDS             12634..>13481
                     /locus_tag="VFO53_04805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET8875629.1"
                     /translation="MILSMTGFAAASAELPGISLAVELRSVNHRYLDVTVKLPEELRT
                     LEPSLRERLASAQKRGKVECRVSLARTVGASGTMAINTERVQQLASAAAAVAGAIPGT
                     PPLTTADILRWPGVMVDATISPEALTEEVDALVQQALSELTSARAREGGKLRAVLEAR
                     CADIETQVARVAPRIPALHAAYLSKLAARLADAGLDANEDRLKQELALFATKIDVAEE
                     VARLVTHVAEVRRVLGQGGSVGKRLDFLAQELHREANTLGSKSVDAELSQVSLELKVL
                     IEQMRE"
BASE COUNT         2054 a         4566 c         4656 g         2205 t
ORIGIN      
        1 ctcggccaca gcgtcggctg cgaccgcctc gaccgtggtt tcgggcgcga cctcgacacc
       61 ccgcgcgtcg gcttcgatcg actcgaccgg cacgtcgtgc tcatcggcga cgagctccag
      121 ccgatcgctc ccttcttcgg cgggaatgag cggctcggcc ggcggcaggt tcgcgagcgc
      181 ctcctggcgc agctcgtcga gctcggtggc aatctcgaag gcttcgcgct cgtcgcccgg
      241 cgtgaagccg tcgcgcgcct tgacgcggcc gacgaagtgc gcgagccccg cggtggcgcg
      301 tgcgagcacc ggctgcgcaa tcccggggaa cggcgcgccg tgctccccga gtgcgagcag
      361 cgcctgctcg agcgcctgcg cggtggtggc gatcagcgcg atgccgccgg tgcgatggat
      421 gccgcacagc gtgtgactcg cgcgcaccat cgtcgcgatc ggccagtgat cgggatcgaa
      481 ctgcagcacc gagacttcgt gctgcagcag cgcaacgtgc tggtcggcct cgtcgcacag
      541 gattcgccag agcgacgacg acaaccgcac gtcgccaacc gtcagttcat cggattcggg
      601 cgccgcggcg ggcgcgcgcg gcgcatcgct cagcagcgtg aggtcgggcg tgtgcgccga
      661 tgacgtgcgg gcgcgtgggt cggcgggcag cgtcgacgtg ttatcggcaa cgacgcgcag
      721 caccggtttc cccgcgtcgc gcaggtcgag cggaaggccg acgtcgatgg tttcgcccat
      781 cgactcctcg agcacggtga cccgcggcgc ggcgccgcct tcgatcggcg cgccgagctc
      841 gggaaaggtg acgagctcga gatccggaag ctggggcagt tcgtgcgacg aacgcgtgtc
      901 gtcgctcgga acctcgtcgg ccgccaacgg gaattcggcg tcgagggtct ggccgggtgc
      961 tgcgaattcg agacgcgcgg cctcgtcgat cggcgcggga aactcgatgt ggccaccggt
     1021 ctcgcccgcg aacggcgtga acgtctcgac gccatcggcc gggaattcaa tgcgcggcgg
     1081 cgacaccggg ccgatcggat gctctgccgc gacgggggaa ggctttgccg gggcgaccgg
     1141 cggcagcgaa agcaccgccg acgacgcacc cggccattcg gcttccacgg cgcgcattgc
     1201 cgacaacagc ggcgatgcgt cggtcgcgaa gcgaccgctt tcccggagct cgcgcaccca
     1261 ctggcggaac gagctggccg cgacgccgat cagtgcgagg aacgccggcg tcacccgccg
     1321 atcctcttcg agcagccggt tatggatgcg ctcgacgccc cacgcgagct cgcccagctc
     1381 ggtcaggccg accatccggc cgctgccttt cagtgtatgg aactgccggc gcaccgtgac
     1441 gagcgcgtcg cggtcggcgg gcgaatgctc gagcacgcgc aggttggcgt cgacggcgtc
     1501 gagcacctcg ccggcctcgg tcaggtagat gtcgaggatc tcggcgtcga gtgcgcgcgc
     1561 atcggtggcg agcagccgct gcgtttcctc ggacagcgcg ggcgccggtg tcgcacccga
     1621 ctcgacgatg aggtcgacgc tcgccgcaag tgccgcctcc gcgccgccgc cgtcgatctc
     1681 gcggatcgcc gccttcacct gcgcggcgag ctcggcgtcg ccgatgaggt cggcatcgtc
     1741 gcgcaggctc acgagcttgc cgcgcaattc ggcgcgcgcc gatgcgtcgg cgggggcgtc
     1801 gcggacgtcg gccagaaggc gcggcagcgc ggcacgcagc tcggcgaccg aatcctcgag
     1861 cgactgcggc tcgacgacct cggcgcgcgg cgcaacgcct tcgcgcttcg cgatcagcgg
     1921 cgcgatgatc cgctcgcgct cgggccgctg atgcaggacc gcgtcgatgt agaagcccag
     1981 ccccgacagc gattcggcaa gaagctcgag cccgtcctcg tcgaccgcgg ttgccgggtc
     2041 ggcgtagctt tcgatctgcg cctggcaaag cgccagcaga cggtcggcgt cgtcgagctc
     2101 gagcatgcgc agcgcgccgc ggatctgctg gctgtcgcgc gtgaggccgc caagctcgag
     2161 gcggcgcgtg tggtcgcgga agaacgcgtc gagcacctgc tccatgtgcc tcaggttcgc
     2221 ctgcacttcg cgcatcacct ggccgagcag cacgcgctcc tgcgcgcggc gcgacatctc
     2281 gtcgagcgcg ggcgccccgt gcgacggtgc ctcacccgtg tgcacggcag cgaggcgcgc
     2341 aagcatcgcg tcgacctggc ttgcgaaatc gggcgacagg ttgccgaagt tctcgaatgc
     2401 gctttcggcg agcagcaacg ccgtcgcgaa ttccatggcg atcggctcgg atgcgccctg
     2461 cggcggcatc gccgccagac gttcggcgag tgcggcagtc agcttcgcaa gcgccggatg
     2521 cccgatctcc tgtgcctttg catgcgcgga ggccaggagc tgcgtgagct tcggcaggct
     2581 ttcggcgcgt cccgacgccg ccttcagcca cgcgtccttg gcgctcgcga gctgctcgcg
     2641 cgcctcacgc aacaatggct tcacgcgcac gatgtcggca tcgagcgctt ccggcgtggg
     2701 cagcagcgct gcaagctgat aggcgcgctg cacggcctcg atctgcggcg cggcaggggc
     2761 cgcgatcgcg atgtagtaga gcacctcgcg gcgcagccgg tcggcaacct tcgcgctgcc
     2821 ctcggtgacg cggcgaatct gcatgtcgat ccgcgcaacg agctgcttcg cgccgaacga
     2881 cggcgcaagc gcgttcgtct cgagggcctc gagcagcgcg ccggccgtcc accagaacgc
     2941 gcgcagattc gattgcgccg tgacgctctc gatggcagcg acggcttcgc gcatcatcgc
     3001 agcaccgctc gcgttgccgc gcagccattc gagaaggccg cgctgatact ggcgccgctc
     3061 cttcaacaga tgcgacgaaa gccgcgccgc cggcacgatc tcggcaggcg ttccgcgcgg
     3121 cgggcgcacg ttgaggtcgg gatagaacag gtcggtgggc gccgccgcct cctcgccgcg
     3181 cgccttgcgc atggcctcgt attcaacggc gagggcgagc gggatcggcg gtgcgccgtt
     3241 cgtcacttca tcgagaaaca cgcgcaggcg gttgcatgca tggtcgacga cggcgacggc
     3301 gtgcggcagc gcgtcgggcg cgagctcctc catgcgtccg agctggcgct cgatttcgcc
     3361 ggtgaaagcg acgacgccgt cgagtcccac catctggatc gcgccggcgg cctggtgaat
     3421 gtgcgagcgc gcatgcttga gcgacgccgt atcggcgggg ttggcgcgaa aggccgcgag
     3481 cgcgccggtg ccgcgcgtca acgcgtcgtc gatctcgccc tgcacccatg tcagcgggcc
     3541 aaggtcgaat tccattgcgg tttcacccaa cacgcgcgcg ctctccatca cggcccgatc
     3601 gacggaaggt tcagaccttg aagcccgcga ccgaactgcg cagttcggcg gcgagcttcg
     3661 tcaactgtcc gatcgacacg ttcgtctgct tcgtgccttc ggtcgtttcc tcggaaatgc
     3721 gcaggatgcc ctgcatgccg cgcgtgacgt cggtgaccga cgccgactgg cgctgcgtct
     3781 gcgcggagat gcggccgatc agctcggcga gctcgcgcga cacgcgctgg atctcgagca
     3841 gcgcctggcc cgcggcgtcg gacagcttcg cgccttcgac gacgccgacc gtggattttt
     3901 ccatcgccgc cacggcatcc tgcgtgtcgg cctgaatcgt cttcacgatc gcctcgatct
     3961 gcttcgtcgc ctcgccggag cgctcggcca ggcgctgcac ttcctcggcg acgacggtga
     4021 agccgcggcc cgcctcgccg gcggaagccg cctggatcgc cgcgttcagc gcgagcacgt
     4081 tcgtctgctc ggtgatgtcc gcgatgagct cgacgatctc gccgatctcg agcgacgatt
     4141 cgccgagccg cttgatccgc ttcgacgtct cctggatctg cgagcggatg tcgttcatcc
     4201 cggagatctg gttctgcacg gcgctgccgc ccttctctgc cgcggcgagc tgcgtctgcg
     4261 ccaccttcgc cgactgcgcg gcgttgctgg cgacctcgtt gatcgaatcg gccatcgtca
     4321 gcaccgacgc ggacgcctgc tggatctcgc ggttctggcg ctgcgacgcc tcgtagagcc
     4381 ggttcgagat cgcctgcgtt tcctgcgtgg ccttcgtcac ctggtcggtg gccgaattga
     4441 tgccgcgcac cagcgcgcgc aattcctcga tcgtgaagtt gatcgagtcg gcgatggcgc
     4501 cggtgacgtc ctcggtgacc gacgcctgca cggtcaggtc gccgtcggcc aggttgccca
     4561 tctcgttcag cagccgcagg atcgcttcct ggttgcgctt gttctcgtgc tcgctgtcct
     4621 gtgcgcgcaa acgcgcgtcg tcgaggaaca ccttcgcgat cagcgcgagg cagccgagcg
     4681 cgacgaggcc gaaggcgatc gcgagcccga gcgtcagcgc gtgcgcctgt gcgccgttct
     4741 cgtactgcgc ggccagcgtc gtggtgtcgt cgagcagcgg ctcggcctcg ttgttgatgc
     4801 tgcgcgccgc ctgcttcgcg acgacgagat tcgccatgtt ggaaaggatg gcattgacac
     4861 cgctctcgta gtcggtgaag cgcctggcga gctcggcgag cgttgcgcgg gcgtcgtcgg
     4921 agcgcgccgg cgcgaggcgc agcgcgtcgc tgcccttcgc gagaccgttg acgacgtcac
     4981 gaaacgccgc gtcgtccttg ccgaacagga acgcgacctc cggatcgatc tcgttggccg
     5041 acgcgagcgt attcgcgttc ttcgcgatgc gctgcgacag cacggcaagc tggttcgcgt
     5101 aatcgacctc gcgcaggctg ccgcccgcct ggccgatctg ctgcgccgcc tgctgcgcga
     5161 gctcgagcaa tccgctgttg ccactgttga tggcggcgag gccgcgcgcg agcgacgtga
     5221 ggctcgcttc gttgtcggtg agccgcttcg cggcggcgtc gatgcgctcg aagcgaccct
     5281 tgatcgtgcc aagcgtcccg gcggcggcgg gatcgtgggt cgggtcgagc gccacgccct
     5341 tgaatgcgcc gttgccggtc agtgcatcga gattggcctt gaagcggttg cggctgtcgc
     5401 ggatcgcggc gaacgcggcc gcttggccct gcgccgcaag cgcggaacca cgcgcgagcc
     5461 gctgcgacag catctgcatc tcggtggccg ccgacgaatg caacgcctgc tgcgacgcgg
     5521 cgcggttgtc ggcgtacacc atggagaccg cgagagcgac gaacgcggtc gtcagcaccc
     5581 cgagtatctg gaactggcgc acgaccggca agccgccgat cagcggcagc ttcgacggcg
     5641 gcggcgcgtt gttgctcgcc gcacgcaact gctccgccac cgtgatcgat gtcaccggat
     5701 cgaagccgac cggcgacgtg atgcccatgc gcacctgtgt ggtcggcacg tcgaagtcgc
     5761 tgttctccgc cgcatgcggc tcgccgccga acagcttcgt catcttgagt gccattgcga
     5821 tgcctcccgc gccacgaccg gggtcgttcc cgatgcgctc gattgaacta cgctccgcgc
     5881 ttcgcgcttc gcgctcatgc gccgacctgc aaaaacgccg ggtcccgggc aagcatcccg
     5941 aggtcgattt cctgccatgc gagcgcctcc gcatcgcgcc agcccgcgac ctgccacgcc
     6001 ggtgcgcctt cggcgacggc atcggtcgtg aattgcgcga ggttgcgcaa gccgaggacg
     6061 cgttccacga cgatgccgac gctctcgccg ccgctacgcg tcgacagcag gatgagccgc
     6121 gaaatgccgg cggtgctcac gggcgcaccg ccgaggaaag ccggaaagtc gacgacgctg
     6181 aacagattgc cgcgaatgtt cgcgagtccg agaaaccacg gtttcgtgag cggcaccgga
     6241 acgatcggcg gcaccgcgac cacctcgccg gcctcgccga ggcggatcag ccagcgcttg
     6301 tcgccgcacg cgatgccgag ccgcgacgat tcgacctgcg cggccgtctt gctcgcgagc
     6361 cgcgaggcaa gctccctctg gaacgagcgc agatcgaggc gggcggcgcg cgacatgtca
     6421 gttgcgggga agattcttca tttcagccga gcgaggcgat cttcgcgagc agcacctcgc
     6481 ggtcgaccgg cttgacgacg taatccttcg caccctggcg aaggccccag atcttgtcgg
     6541 tttcctggct cttcgacgtg cacatgatca cggggatgga cttggtctcg ggatcgcggc
     6601 tgattgcgcg cgtggcctgg aaaccgttga ggccgggcat cacgacgtcc atcaggatga
     6661 ggtcgggctt gaccgtgcgc gccttgacga tggcgtcctc gccgttctca ctcgagatga
     6721 cgtcgtagcc tgccttcgtc agcaggtcct tcagcatgtg gcgctcggtg gccgaatcgt
     6781 cgacgacgag gatgcgcttg atgcccatcg tggctccctt ccgcccggtt tgcgtcgaat
     6841 gggtttgtgc gaggaccgcg cgagcggtga tcggattgct catgcgtgca attcccggga
     6901 tgggcggttc gaaagacgga tcatggaacg gaggggtatc ggcgccgcta ggcagccacg
     6961 gcgcggacgt gcgcggcgac cgctttcagc aggctttcct tcgtgaacgg cttcgtcagg
     7021 tactggtcgg agccgaccat ccggccgcgc gcgcgatcga agagcccgtc cttcgacgac
     7081 agcatgatca ccggcatcgc atgaaaacgg ctgttcttct tgatgagcgc gcaggtctgg
     7141 taaccgtcga ggcgcggcat catgatgtcg acgaagacca ggtccggctg gtgatcggcg
     7201 atttttgcca gcgcgtcgaa gccgtcctcg gccaggatca cggtgcaacc ggcctgcagc
     7261 aggaagatct cggcactgcg gcggatggtg ttcgaatcgt cgatcaccat caccttgatg
     7321 cctgaaagcg gacttccgcc cggagtgtcg ttgcccatcg gcgccttgta cggtccccca
     7381 cgcttcgcgg ctactcgcca ctgcgcgccc aggaacgacg ctgcggcgct gcatgggatg
     7441 ctcgcggcat gcggtgccgc accggacccg aagcgagtct gccatggccc gggcggcttg
     7501 cctagagcat aaagcttgcc ggaatcggca ccgcgctccg attccgacga aaacgcaaca
     7561 acacatcata ttttcccgcc agcggcttgc cgttgcgcgg catgacgtgc cgcgtctcga
     7621 cgccggttcg cgccgcgggg gccacgcagg aaagcggcag cgtccggcgc gaaaaccgcg
     7681 tcaaccgccg agaaacatcc gatacgcggg gttcgccgat tcctcgacgt agtcgtagcc
     7741 gaggccgtcg aggaaggcgc gaaaggcgcc gctgtcgcgc ggcggcacct gcatgccgac
     7801 cagcacgcgg ccgtaatcgg cgccatgatt gcgatagtgg aacaggctga tgttccagcg
     7861 cgcgctcatg ctgtcgagga agcgcatcag cgcgcccggc cgctccggga actcgaagcg
     7921 gtagacgatc tcgtgctcgg ccgccggcgc gtggccgccg accagatggc gcacgtgcag
     7981 cttggccgtt tcgttgtcgg ataggtcgga cgcctcgata cgggcgcgct tcaacgcggc
     8041 cagcagctgc tcggtttcgc gccggttggc aacctcgatg ccgacgaaca ggtgcgcaac
     8101 gcgcggatcg gcgtagcgat agttgaattc ggtgaccgag cgcctgccga ggagcgcgca
     8161 gaactcgcga aagctgcccg gccgctccgg aatgctgacg gcgaggatgg cctcgcgttg
     8221 ctcgccgagc tcggcccgct cggcgacgaa gcgcagccgg tcgaagttca tgttggcgcc
     8281 gcaggcgatg gcggcgaacg tctgtccttt cacccgcgtt cgctgcaccc acgccttgac
     8341 gcccgcaacg cccagcgcac cggccggctc gacgatcgcg cgcgtgtcct cgaacacgtc
     8401 cttcagcgcg gcgcagatgg cgtcggtgtc gacgacgacg acgtcgtcca cgtatttgcg
     8461 cgcaagccgg aaggtctcct tgccgacctg cttcacggca acgccgtcgg cgaaaagccc
     8521 cacgtgcgcg agcgtgacgc gccggccggc ggccagcgag cgcgccatcg catcggaatc
     8581 ctcgggctgc acgccaatga tcgccacgtc gggacgcacc cgcttcacgt acgcggcgat
     8641 gccgctcacg agacccccgc cgccgatcgc gacgaagatc gcgtcgagcg gtcccgggca
     8701 ttggcgcagg atttccatgc cgatcgtccc ctgccccgcg atgacgtccg gatcgtcgta
     8761 cggatgcacg aacgtcgcgc cgagctcctg ctgcagcgcc tgcgccctgt cgtacgcgtc
     8821 ggaatacgaa tcgccgtgca ggatgacgca tgcgccgcgc gcctcgacgg ccgcgatctt
     8881 gatccgcggc gtggtcaccg gcatcacgat cgtcgccgtg cagccgagcc gctgtgcggc
     8941 gagggcgacg ccctgtgcat ggttgccggc cgaggccgcg atgacgccgc gcgcccgctc
     9001 ggcggcgctc atctgcgcca tcttgttgta ggcgccgcgc agcttgaacg aaaagaccgg
     9061 ttgctgatcc tcgcgcttca gcagcacgtg gttgccgagg cgcgcggaaa gcgcgggtgc
     9121 cacatcgagc ggcgtctcga tggcgacgtc gtagaccctg gcggtgagga tcttctgcag
     9181 gtagtcgacg ggcatgcgtc accgcggccg gcaagaccgc atgaggaaag ccgtcgaggt
     9241 tatcaggcgc tgccgggacg ttgcccggcg cggtgaatca ggtgccgtcg cggcggtgcg
     9301 acaggaccgt cacgccgagc gggttcgtac gaatcgcgct acgggtgctg ccagggccta
     9361 aacaaaatcg gggccgatcg cgaccttcag cacaacggct gccggttcct gatgcacgcg
     9421 gttggaaagc caccacacgg cgcccgtatg catggcctgg tcgatttcgt cgccggccag
     9481 aaggaacgac gccacccggc cgagcgttgg ctgatggccg acgacgagca ccggcgcacg
     9541 cgcttccggc cagcccgcgg tggccagcac ggctgcaacc gatgcaccgg tgccgatttc
     9601 cggcaccgac tcgaatttgc ggtcgagcgc ctgagcggtc tgttgcgcac gcagcgccgg
     9661 cgacacgatg atccggcagc cggcaggcag ccgctcgtcc agccagtgcg ccatcctcgc
     9721 cgcctgcttg cggccgagtg gcgtgagcgg gcgcagctcg tcgggcattc cggacgcggc
     9781 gtcgcaatga cgccagagga cgaggtccat cgcgcggttg cgccgcggtg cgcggttggc
     9841 gtcaggcgac cgcgatcgcg accgacgctg ccgcctcgag cgcgagccag acgcagcccg
     9901 acttgcactc gcgcgcgagg gcgtcgagat acgcgcggtg caaagcgagc tcggcgtcgt
     9961 cgggcacgat ccgatggacg acgtagcgcc cggcgcgcat cgccgcgccg tcgatcgcga
    10021 gcccgcccgc cggaaccggc ggcgcgtaca tgtcgatgac gagcgtttcc tgcccgcgcg
    10081 tcatcgcgag gtacacctcg gcgaggagct gcgcgtcgag cagcgcgccg tgcagcgtgc
    10141 gctgggcgtt cgacacgccg aagcgctcgc acagcgcgtc gagattgttg cgcttgccgg
    10201 gaaacgtgtc gcgcgcgagc gcaagcgtgt cgatcagcct gccatagatc gcgtcggact
    10261 tcggcaggcc gcacaggccg aactccgcgt cgaagaacgc gacgtcgaac ggcgcgttgt
    10321 gaatgatcca ttcggcgcca cacgcgaagt cgacgaactc ggcggcgata tcggcgaagc
    10381 gcggcttgcc gcgcagatcg tcgcgcgtca ggccgtgcac ctcggtggcc gcggcatcga
    10441 tgtcgcgatc tggatcgaga cggaaatgaa gatggcgtcc cgacagccgc cggttcacga
    10501 cctcgacggc ggcgatctcg atgatgcgat gcccctggcg tgggtcgagc ccggtggttt
    10561 cggtgtcgag gaggagctgg cgcatgcctt atttatttcc ggtacttctg tatcgtcgac
    10621 tggatttccg cgattgcgcg ctcgatttcg tcgtcggtca ggtcgcgctg gccgcccgca
    10681 cggtcggcga gcgtgaccgt tgcgtcgact tgcgtctcga actgtccgag cgctaaattt
    10741 tccgtggtcg tcggttcgtc ggcaagcgtc tcggggcccc agcgcacgat gatcgccgac
    10801 acgttgtcgc cttcgtcccc gccgcgcttc tcggcttcgc gcatgatctg cggcgcggtg
    10861 gcgaggatcg gcgtcgacgt cagcatcgtc gccatttcgc cgtgcggata gacgctccac
    10921 aggccatcgg tgcagaggat caggaggtca ccgtccagga gctgcgtgcg ccgtgacagg
    10981 tcgatcaccg ggtcggccat gccgccgagg cagctgaaga tcttgttgcg gtcggggtgc
    11041 atcagcacct cctccggcgt gatcagcccc tggtcgacga ggtactgcac cttcgagtga
    11101 tccttcgtcg atgcgatgag ccggccctgg cgcagcaggt agaagcgcga atcgccgacg
    11161 tgcgtccagt acgcgtgccc cccctgcacg acgacggcga cgcacgtggt gcgcggcgtc
    11221 tcgagcatcg agaactggtt cgcgtacgag ccgagtgccg catgcgcgcg cagcatcgtc
    11281 tcctgcatga acttcagcgg attgcgcagg atcggcttcg cttcctggtg gaagcgctcg
    11341 atgaaaagcc gcacggcgat ctgcgcggcg atctcgccgc cgacgtggcc gcccatgccg
    11401 tcggcgatca cgagcagcag cgtctcgcgg ctgtaggtgt aggcgatgcg gtcctgattg
    11461 acctcgcgcg cacccttgcg cgattcctgg aagatcgtga aacgcatggt gcgagacagc
    11521 gctagacgcc gatctccgag aatagcaggc gcttgaggtt cgcgaagaac gtgagcttgc
    11581 gcttgatcgg cgcgatgtcg cggatcgcgc gctgcagtgc gaacacgctt tgcggtcgct
    11641 cgagcgggtc gagccgcagg caccagtcga tcacctcgag caggttgtcc gagtactgcc
    11701 ccgaccagat cttcttcgcg gagacgaggt gatcctcctg cacgcgggca tcggccgcct
    11761 gcggcgcgaa cgccgcgagg cacgcgaaca tcgacgcacc ggtgccgtag acgtcggtcc
    11821 acgggccgag ccggtcgtgg tcgcgatact gctcgggcgc ggcaaagccc ggcgtgtaca
    11881 tcggcgcgag cttcggccgg ttcgtggtca gcgtctggcg cgcggcaccg aagtcgagca
    11941 gcaccggact gccgtcggtg cgcaggtaga tgttcgccgg cttgatgtcg aggtgcagga
    12001 tcttgtgcgt gtgcacctcg cgcagcccgt tcagcagctg catgaacacc tggcgcacga
    12061 agcgctcgga catctggtcc tggcgcatct ggatctgctg ctgcagcgtg cgcccgcgct
    12121 cgtagcgcat caccatgtag acggtttcgt tcgcgcgaaa gaaattgagc acgcgcacga
    12181 cgttcggatg gttgatcttc gcgagcgccc gcccctcctc gaagaagcac ttcatcccgt
    12241 agcggaacga cgtcaggttc tcggccgagc tcgcacgcac gatgtcgcct tcggtgcgca
    12301 gcaccagcga cgacggcagg tattccttga tggcgaccgg ctggccggtc tcgtcgaacg
    12361 cgcgatagac gatcgagaat ccgccgcggc tgatctggcg atcgatccgg taattgagga
    12421 gcgcgtagcc gccgggcagc gcttgattgg tgacgggcat cgtggatggg caagcggcgg
    12481 gcgcgcgcgg tgcactcaca ccgcaagtcg cgtgccaaga ctccccgttc gccttgcgcg
    12541 aggagcgcag aacgcataca ttgtcccgat gaccacgccg aaagtaaagc cgcaatcgca
    12601 ggcaacgccg gccgttgccg cgcgctctac ccgatgattc tcagcatgac cggcttcgcg
    12661 gcggcgagcg ccgagctgcc cggcatctcg cttgctgtcg agctccgctc ggtcaatcac
    12721 cgctacctcg acgtcaccgt caagctgccc gaggagctgc gcaccctcga gccgtcgctg
    12781 cgtgaacggc tcgcgtcggc gcagaagcgc ggcaaggtcg aatgccgcgt gtcgctggcg
    12841 cgcaccgtcg gcgcgtcggg cacgatggcg atcaacaccg agcgcgtgca gcagctcgca
    12901 agcgctgccg ccgctgtcgc aggtgccatt cccggcacgc cgccgcttac caccgccgac
    12961 atcctgcgct ggccgggcgt catggtcgac gcaacgattt cgcccgaggc gctgaccgag
    13021 gaggtcgacg cactggtcca gcaggcgctg tccgaactga cgtcggcgcg ggcgcgcgag
    13081 ggcggcaagc tgcgcgcggt gctcgaggcg cgctgcgccg acatcgagac gcaggtggca
    13141 cgcgtggcgc cgcgcattcc cgcgctgcac gcggcgtacc tctccaagct cgccgcgcgg
    13201 ctcgccgacg ccggactcga cgcgaacgag gatcgcctga aacaggagct tgcgctgttt
    13261 gcgacgaaaa tcgacgtcgc cgaggaggtg gcgcggctcg tgacgcacgt cgcggaagtg
    13321 cggcgcgtgc tgggacaggg cggcagcgtc gggaagcgac tcgactttct tgcgcaggag
    13381 ctgcaccgcg aggcgaacac gctcgggtcg aaatccgtcg atgccgagct ttcgcaggtg
    13441 tccctcgagc tcaaagtgct gatcgagcag atgcgcgagc a
//
LOCUS       DASSVX010000106         6084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_753823, whole genome shotgun sequence.
ACCESSION   DASSVX010000106 DASSVX010000000
VERSION     DASSVX010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753823"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFO53_04810"
     CDS             complement(<1..644)
                     /locus_tag="VFO53_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HET8875630.1"
                     /translation="MSRILIFAEAVTLAHVARPIALSRILRELGHEVCIAAAPAADRW
                     LAGENVARQRIGSIDSARFLRALARGVPAYGRETLKRYVEDDLRAIAAWPPDIVIGDF
                     RLSLYISARLAHKPYGAIANAYWSRRYWTGVDAPDIAALNWLPRSMANSVFRAVYPAA
                     FALHALPFHAVCRHFGVAPPGRDIRDIYTASDVTAFADVRSFYEPSPAPDAPLF"
     gene            700..1920
                     /locus_tag="VFO53_04815"
     CDS             700..1920
                     /locus_tag="VFO53_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET8875631.1"
                     /translation="MLTTELKLAVRNLARERKRTLISLAAIVVGVVGYLLAGGFIDWI
                     FWAIRESAIDNGLGHIQISAKGYRDAGQADPGAYMLPANGPQKDAIARLPGVVAVSPR
                     LMISGLVSHGEVTVPFAGEAVEPDVDARISKDIAVDGEPLDAASPKGVILGRGLARAL
                     NVKRGDVLAFITTGASGGMSGVEGIVRGVYSTGVKAFDDVAVRMPLALGQQLMRTKGV
                     HLWVVGIDDTAHTQAVASAISPILADSGKEMRTWLDLSDFYRKTVTLMSRQMLVVAVL
                     IAIILVLGIANLLTMSTLERTGEIGTMLALGTPRSSVLRMHFMEGLLLGLAGAIAGLA
                     IGAVLAAVISWIGIPMPPPPGRDAGYRAEILLSLPIILDAVALALASAAIAGAYPAWK
                     ASRMPIVDALRFNR"
     gene            1930..3144
                     /locus_tag="VFO53_04820"
     CDS             1930..3144
                     /locus_tag="VFO53_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET8875632.1"
                     /translation="MLLSIAVRNLTRQRTRALLTLASVGFGVASLMLAGGFIDDILLQ
                     LRDATIHSQLGHFEVFAPGYVDAGRREPLAHTLADSPGAIRAIRAIPGVATIAPRLSF
                     SGLLSNGRTDANVSIEGIDPAAEQAIGTAVTTIAGSSLKAVSGPAVVVGDGLAHTLNL
                     RVGDTVTVLASTREGALNTLDAPVAGIFRSPFRDYDERAVRISLRDAQDLVATDSVNS
                     LAVLLGPDASVDEAVAAARHALPPIRYDIRPWWSLADFYEATASLYRRQFLVLLTIVA
                     AMVLLGVANSVNMSLHERQSEFGTVRALGYGASAVFRQIIVESALLGVAAALAGIVLG
                     LLLAWAISAIGIDMPPPPNSDVGYRAAIRVSFSNVALAAAIGVMAAVVGAILPARRLA
                     RMPIVDALRHAI"
     gene            3154..3891
                     /locus_tag="VFO53_04825"
     CDS             3154..3891
                     /locus_tag="VFO53_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875633.1"
                     /translation="MLIRLESVTKTYPMGVQTINALRNVSLNIEDGAFIAIAGPSGSG
                     KSTLLNILGLIDRPTSGRLLIDDREVSGRSPDDLAALRARSIGFVFQTFNLLPVLTAE
                     ENVEYPLLQFRELKTKERRERVAHFLEVVKLQAHAKHRPNQLSGGQRQRVAIARALAT
                     QPRVILADEPTAHLDHRTGSRILRLMRRINRRSGTTFVFSTHDPRVLEIADRRVDLED
                     GAIARLGVRTRDTWTYAAERTPTVTGE"
     gene            complement(3878..4981)
                     /locus_tag="VFO53_04830"
     CDS             complement(3878..4981)
                     /locus_tag="VFO53_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein MoeY"
                     /protein_id="HET8875634.1"
                     /translation="MPASVTVAEDVLDVARFAPSGDNSQPWRFVLRGADAFDVYGYDT
                     SEHCVYDLDGWASHLAHGMLLETIGIAATRHGRKANVELPGDDSVRPLRYRVRLDADP
                     AAREDRLLDAIRARSVQRLPMSMRQLTTNERDALERAAAPFRVRWFETLKARIAIAAL
                     CMRNARIRLTIPEAYAVHRAVIDWHAITSEDRLPAASLGAGRMLLATMHHAMASWQRL
                     DRLNRWTGTLAPRIALDFLPGVRCSAQFALIADAASSRLADRLAAGRATQRMWLIATA
                     MGLQMQPQYTPLVFARYARADRRFSSAQRAHDEARAVAEGLAKMLGSDATRAVWLARI
                     GPARPAGGRSLRLPLSTLIAAEAPDALPALTPL"
     gene            complement(4995..5858)
                     /locus_tag="VFO53_04835"
     CDS             complement(4995..5858)
                     /locus_tag="VFO53_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET8875635.1"
                     /translation="MDAGFDYDVAFSRNLGWVTPDEQQALRGKRVAIAGLGGVGGLHL
                     LALARLGVGRFHLAEFDLFELANFNRQVGASIASLGLPKLDVMMKLARNINPELEIVP
                     FDAGMDANNVDAFLGGVDVFVDGLDFFAFAARELVFGACHARGIPAVTAAPLGMGAAL
                     LNFVPGGMSFADYFGLAHCAQSEKPIRFLLGLAPAMIHRRYLADLSFVDLESGKGPST
                     AMACHLCAGVAATETLKLLLHRGSIVKAPHGVQFDAYRNKLTHTWRPWGWRNPLQRLV
                     AAVARRELARR"
BASE COUNT          991 a         2033 c         2047 g         1013 t
ORIGIN      
        1 atgaagagcg gtgcatcagg ggcgggcgaa ggttcgtaaa acgagcggac gtccgcaaac
       61 gcggtcacgt ccgacgctgt atagatatcg cgaatatcgc gcccgggcgg cgctaccccg
      121 aaatggcggc acacggcatg aaacgggagc gcatgcaagg cgaaagcggc cggatagacc
      181 gcacgaaaga cggagttcgc catcgatcgc ggcagccagt tcaacgctgc aatgtcgggc
      241 gcgtcgacgc cggtccagta gcgccgcgac cagtaggcgt tcgcgatcgc gccatacggc
      301 ttgtgcgcca atcgcgcgct tatataaagc gacaaccgga aatcgccgat gacgatgtcg
      361 ggcggccagg ccgcgatggc ccgcaggtcg tcctcgacgt accgtttcag cgtttcgcgg
      421 ccataagccg gcacgcctcg ggcgagggca cgcaggaacc gcgccgagtc gatcgatccg
      481 atccgctgtc gcgcgacgtt ttcgccggca agccatcgat ccgccgccgg tgccgcggca
      541 atgcatactt cgtggccgag ctcccttagt attcgcgaca gcgcaatggg tcgcgccaca
      601 tgcgcgagcg tcacggcttc ggcgaaaatc aggatgcggc tcaacgatca cacctctctg
      661 cccttggcaa tgcaccgcac gcgagtatag gacgggccga tgcttacgac cgagctcaag
      721 ctcgccgtgc gaaaccttgc gcgcgagcgc aagcgcacgc tcatttcgct cgccgccatt
      781 gtcgtcggcg tcgtcggcta tctgctcgcg ggcggattca tcgactggat cttctgggcg
      841 atacgcgaat cggcgatcga caacggcctc ggccatatcc agatttcggc caaggggtac
      901 cgcgatgcgg gccaggccga tcccggcgcc tacatgctgc ccgcgaacgg cccgcagaag
      961 gatgcgatcg cacgccttcc cggcgtcgtt gcggtttcgc cgcggctcat gatcagcggg
     1021 ctcgtgagcc acggcgaagt cacggtgccg tttgccggcg aggcggtcga gcccgacgtc
     1081 gatgcgcgca tcagcaagga catcgccgtc gatggcgagc cgctcgatgc cgcgtcgccg
     1141 aagggcgtca ttctcggccg cggtctcgcg cgtgcgctga acgtcaagcg cggcgacgtc
     1201 ctggcgttca tcacgacggg cgcgagcggc ggcatgagcg gcgtcgaagg gatcgtgcgc
     1261 ggcgtctatt cgaccggcgt caaggcgttc gacgatgtcg ccgtccgcat gccgcttgcg
     1321 ctcgggcagc agctcatgcg cacgaagggc gtgcacctct gggtcgtcgg gatcgacgac
     1381 acggcgcaca cgcaggccgt agcaagcgcc atctcgccga tcctcgccga ttccggaaag
     1441 gagatgcgaa cctggctcga cctgtccgac ttctatcgca agaccgtcac gctgatgtcg
     1501 cggcagatgc tggtcgtcgc cgtgctgatc gcgatcatcc tcgtgctcgg cattgcgaac
     1561 ctgctcacga tgagcacgct cgagcgaacc ggcgagatcg gcacgatgct ggcactcggc
     1621 acgccgcgga gcagtgtatt gcgcatgcac ttcatggaag gcctgctgct cggtctcgcg
     1681 ggtgcgattg ccgggctcgc gatcggcgcg gtgcttgccg ccgtcatctc gtggatcggc
     1741 atcccgatgc cgccgccgcc cgggcgcgat gcgggctatc gtgccgaaat tctgctgtcg
     1801 ctgccgatca tcctcgatgc ggtcgcgctc gcgctggcga gcgccgcgat cgcgggcgcg
     1861 tatcccgcgt ggaaggcatc gcggatgccg atcgtcgacg ccttgcggtt caatcgctag
     1921 atttcggaca tgctgctttc gattgccgtc cgcaatctca cccggcagcg cacgcgcgcg
     1981 ctgctgacgc tcgcctcggt cggcttcggc gtggcgagcc tcatgctggc tggcgggttc
     2041 atcgacgaca tcctgttgca gttgcgcgat gcgacgatcc actcgcagct cggtcacttc
     2101 gaagtgttcg cgccgggtta cgtcgacgcc ggacggcgcg agccgctcgc gcataccctc
     2161 gccgattcgc ccggcgcgat cagggcgatc cgcgccattc ctggggtggc gacgattgcc
     2221 ccgcgacttt cgttttcggg gttgctgtcg aacgggcgca ccgacgccaa cgtcagcatc
     2281 gagggaatcg acccggcggc cgaacaggcg attggaaccg ccgtgaccac gatcgccggc
     2341 agctcgctca aagcggtgtc cggtccggcc gtcgtcgtcg gcgacggtct cgcgcacacg
     2401 ctcaacctgc gtgtcggcga cacggtgacg gtgctcgctt ccacgcgcga aggcgcgcta
     2461 aacacgctcg atgcgccggt tgcaggcatc ttccgcagcc cgttcaggga ctacgacgag
     2521 cgcgcggtgc gcatcagcct tcgcgacgca caggatctcg tggcaaccga ttccgtcaac
     2581 tcgctcgccg tgctgctggg cccggatgcg tccgtggacg aggcggtggc ggcggcgagg
     2641 cacgcgcttc ctcccatccg ctacgacatc cggccctggt ggagcctcgc cgatttctac
     2701 gaagccacgg cgtcgctgta tcggcggcag tttctggttc tgttgacgat cgtcgcggcc
     2761 atggtgctgc tcggcgtcgc gaacagcgtc aacatgtcgc tgcacgagcg gcagtccgag
     2821 ttcggaacgg tacgcgcgct gggctatggc gcgagcgccg tcttccgcca gatcatcgtc
     2881 gaatcggcgc tgctcggtgt cgcggcagcg ctcgccggca tcgtgctggg cctgttgctg
     2941 gcatgggcca tttcggcgat cggcatcgac atgccgccac cgccgaattc ggacgtcggc
     3001 taccgtgccg ccattcgcgt ctcgttctcg aatgtcgcac tggcggcggc gatcggtgtc
     3061 atggccgccg tcgtcggcgc cattcttccc gcgcggcgac tcgcgcgaat gcccatcgtc
     3121 gatgcgttgc gacatgccat ttgagcggcg gccatgctga tccgcctcga atccgtcacc
     3181 aagacttatc cgatgggcgt gcagacgatc aacgcactgc gcaacgtgag cctcaacatc
     3241 gaggacggcg cgttcattgc gatcgcgggg ccatcgggca gcggcaagtc gacgcttctc
     3301 aacatcctcg gactgatcga ccggcccacg tccgggcgcc tcctcatcga cgatcgcgaa
     3361 gtatcgggac gctcgcccga cgatctcgcc gcattgcgcg cacgatcgat cggcttcgtg
     3421 ttccagacgt tcaacctgct gccggtgttg accgccgagg aaaacgtcga atacccgctg
     3481 ctccagtttc gtgagctgaa gacgaaggag cgccgcgagc gcgttgcgca tttcctcgag
     3541 gtcgtcaagc tgcaggcgca tgccaagcac cggccgaacc agttgtcggg cgggcagcgg
     3601 cagcgggtgg cgatcgcgcg cgcactcgcc acccagccgc gcgtgatcct cgccgacgag
     3661 cctacggccc atctcgatca tcgcaccggc agtcgcatcc tgcggctgat gcgcaggatc
     3721 aatcgccgca gcggcaccac gttcgtcttc tcgacccacg atcctcgcgt gctggaaatt
     3781 gctgaccgtc gcgtcgacct cgaggacggc gcgattgcgc gcctcggcgt gcgcacgcgc
     3841 gacacgtgga cctatgccgc ggagcggacg ccgaccgtta caggggagtg agtgccggca
     3901 atgcgtctgg cgcctcggcc gcgatcagcg tcgacagcgg aagccggagc gagcgtccgc
     3961 ctgccggacg cgccggcccg atacgcgcga gccagacggc gcgcgtagcg tcgcttccga
     4021 gcattttcgc gagcccctcg gcaaccgcgc gtgcttcgtc gtgcgcgcgc tgtgccgagc
     4081 tgaaccggcg atcggcgcgc gcgtagcgcg cgaacacgag cggcgtgtat tgcggctgca
     4141 tttgcagccc catggcggtc gcgatcagcc acattcgctg cgttgcgcgt ccggcggcga
     4201 gccggtctgc gagtcgcgac gaagcggcat cggcgatcaa ggcaaactgc gcgctgcagc
     4261 gaacgcccgg caggaagtcg agcgcgatgc ggggagcgag cgtacctgtc caccgattga
     4321 gccgatcgag ccgctgccag ctcgccatcg catgatgcat cgtggcgagc aacatgcggc
     4381 ccgcgcccag cgacgcggcg ggcaggcgat cctcgctcgt gattgcatgc cagtcgatga
     4441 ccgcccggtg cacggcgtac gcctcgggaa tcgtgagcct gatgcgggcg ttgcgcatgc
     4501 atagcgcggc gatcgcgata cgcgccttca gcgtctcgaa ccagcgcacg cgaaatggcg
     4561 cggccgcccg ctcgagcgcg tcgcgctcgt tcgtcgtcag ctgccgcatc gacatgggca
     4621 gccgctgcac gctgcgggcg cgaatggcat cgagcagccg gtcttcgcgc gccgccggat
     4681 cggcgtcgag gcgcacgcgg tagcgcagcg ggcgcacgct gtcgtcgccc ggcagttcga
     4741 cgttcgcctt gcggccgtgg cgcgtggctg cgatgccgat cgtttccagc agcatgccgt
     4801 gcgcaagatg gcttgcccag ccatcgaggt cgtagacgca gtgctcgctc gtgtcgtatc
     4861 cgtagacgtc gaaagcgtcc gcgccgcgca gcacgaagcg ccagggttgc gagttgtcgc
     4921 cgctcggtgc gaagcgggcg acgtcgagca cgtcttcggc gaccgtcacg gatgcgggca
     4981 cggcggcatg cgggtcagcg acgggcgagt tcgcgccgcg cgacggcggc gacgagacgc
     5041 tgcagcggat tgcgccagcc ccatggccgc caggtgtgcg tgagcttgtt gcggtacgcg
     5101 tcgaattgca caccgtgtgg cgccttcacg atcgaaccgc ggtgcagcag cagcttgagc
     5161 gtctcggtcg ccgcgacgcc ggcgcaaagg tggcaagcca tcgccgtcga tggtcccttg
     5221 ccgctttcga gatcgacgaa cgaaaggtcg gccaggtagc gccggtggat catcgccggc
     5281 gcaagcccga gcaggaagcg gatcggcttt tcggattgcg cgcagtgcgc gagcccgaaa
     5341 taatcggcga agctcattcc tccgggcacg aagttgagga gcgccgcccc catgccgagc
     5401 ggcgccgccg tcaccgcggg aatgccgcgc gcgtgacacg cgccgaatac gagctcgcgt
     5461 gcggcgaaag cgaagaaatc gaggccgtcg acgaacacgt cgacgccgcc gagaaatgcg
     5521 tcgacgttgt tcgcgtccat gcccgcgtcg aagggcacga tctcgagctc gggattgatg
     5581 ttgcgcgcga gcttcatcat cacgtcgagc ttcgggagcc ccagcgacgc gatcgatgcg
     5641 ccgacctggc ggttgaagtt ggcgagctcg aagaggtcga attccgcgag gtgaaagcgc
     5701 ccgacgccaa gacgtgcaag cgcaagcaga tgcaggccgc caacaccgcc gaggccggcg
     5761 atcgcgaccc gcttgccgcg cagcgcctgc tgctcgtccg gcgtcaccca gccgagattg
     5821 cgcgagaacg cgacgtcgta atcgaatccg gcgtccatca gttgcgcgcg ggcaggggcc
     5881 gcgccgatcg cccgggcgtc atcggcatcg acgtcttatt gagcatcacg gaactccatg
     5941 atgatttgca gagctccgtc gttcccgcgg aagcgggaac ccatacatgg tcgccccctt
     6001 tttgccaagc atccgatttg cgatagcgag caggtagtga ttgcagccat acatccggcc
     6061 tttagatcga ggccgcaacg gcct
//
LOCUS       DASSVX010000107         3637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1520403, whole genome shotgun sequence.
ACCESSION   DASSVX010000107 DASSVX010000000
VERSION     DASSVX010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520403"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /gene="hemF"
                     /locus_tag="VFO53_04840"
     CDS             <1..792
                     /gene="hemF"
                     /locus_tag="VFO53_04840"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HET8875636.1"
                     /translation="GGGVSMSIEDGEVFERAGVLFSHVTGTALPASATAHRPQLAGRA
                     WEALGVSLVLHPRNPYAPTVHLNVRHFCAVSHDARDLAPVWWFGGGMDLTPTYGFVED
                     ARHFHSVCRDALAPHGAELYPRFKRACDAYFHLKHRGEARGIGGIFYDDFSEGGFGRA
                     FAMTKSVGDRFLDAYAPIVQRRRDMPYGERERDFQAYRRGRYVEFNLVWDRGTLFGLQ
                     SNGRTEAILLSMPPIARWRYEWAPLPGTPEAALYTDFLPVRDWLA"
     gene            789..1643
                     /locus_tag="VFO53_04845"
     CDS             789..1643
                     /locus_tag="VFO53_04845"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET8875637.1"
                     /translation="MSGDPRPAADRPDDPLAERRIGGEQVYGGTLLDVRRDRARMPDG
                     SDAVREYIVHPGAVLVVPVHDDGRMIVERQFRYPHNRSFLEFPAGKLDPGESPLESGV
                     RELVEEAGYRAQLWLRLGTIHPVISYSTEAIVLYAARGLVHVGARLDPGEFLEIVECT
                     ESALYEAIDAGRVTDAKTIAALALYSRWSAATTRSVRLSITGRVQGVGFRDWALRAAA
                     RAQLRGFVRNRGDGSVEAHLQGERRACDRFIDDCREGPRACRVERIEVERAPFDASLA
                     DFRVARSN"
     gene            complement(1665..2375)
                     /locus_tag="VFO53_04850"
     CDS             complement(1665..2375)
                     /locus_tag="VFO53_04850"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8875638.1"
                     /translation="MPLEHAVGSIGTDTPARTERRRGDDTRSRLLDAVEGLIAEYGFK
                     PLTHRTIASRAEAHVALLNYHFGSKEQLIEEALARRAGQLLAMQHDALAALRERGDFT
                     VEDVLWAYGRPFASLDRDGASPWRNYLCLVARLAGHDPSDSIMLRHFGSVYRECLNAL
                     RHALPGVDDASLAAGFRNCRLLLEHDALARCKASGNPPPFTPSPEELIAFLAGGMHAL
                     ATHAHARQAQGRTEETAH"
     gene            complement(2514..3176)
                     /locus_tag="VFO53_04855"
     CDS             complement(2514..3176)
                     /locus_tag="VFO53_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875639.1"
                     /translation="MPLQHALHWYRDAMRLWKRGPASFCAIALVVIVANIALTLVPIA
                     GIVLAQLLLPLIECGLLYASLAADRGDRPRLRHLVAIAAAPARAQAAIIAAALIVFAA
                     EALVANAVGGFNMLAPAGNAESISGMTLVATYAAGVVVSLPLTFVPFAVLFDGAGVRT
                     AFAESGAAFARNASPLLLYGALSFVLLMIGLATSGIGLLLALPWSAAASYAAWKDVFG
                     VA"
     gene            complement(3272..>3637)
                     /locus_tag="VFO53_04860"
     CDS             complement(3272..>3637)
                     /locus_tag="VFO53_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8875640.1"
                     /translation="RVFALFPRLKERRRQTAGTLSGGEQQMLAIARAMMSRPRLLLLD
                     EPSMGLAPLMVQKVFETILSVASEGVTILLVEQNAKLALDVSDRGYVMESGEVTLAGK
                     AADLLHDPKVRAAYLGEAA"
BASE COUNT          567 a         1224 c         1289 g          557 t
ORIGIN      
        1 ggcggcggcg tttcgatgtc gatcgaagac ggcgaggtat tcgagcgcgc cggcgtgctg
       61 ttctcgcatg tgacgggaac ggcgctgccc gcatcggcca ccgcgcaccg gccgcagctt
      121 gccgggcgcg cgtgggaggc gctcggcgtg tcgctcgtgc tgcatccgcg caatccgtat
      181 gcgccgacgg tacatctcaa cgtccggcat ttctgcgccg tttcgcacga cgcgcgcgac
      241 ctggcgccgg tctggtggtt cggcggcggc atggatctca cgccgacgta tggcttcgtc
      301 gaggatgcgc gtcactttca ctcggtgtgc cgcgacgcgc tcgccccgca cggcgccgag
      361 ctgtatccgc ggttcaagcg cgcgtgcgac gcgtacttcc atctgaagca ccgcggcgag
      421 gcgcgcggga tcggcggcat cttctacgac gatttcagcg agggcggctt cgggcgcgcg
      481 ttcgcaatga cgaagagcgt cggcgaccgc ttcctcgacg cgtacgctcc gatcgtgcaa
      541 cgccggcgcg acatgccgta cggcgagcgc gagcgcgatt tccaggcgta ccggcgcggc
      601 cgctacgtcg agttcaacct cgtctgggat cgtggcacgc tgttcgggct gcagtcgaac
      661 gggcgcaccg aggccatcct gctgtcgatg ccgccgattg cgcgctggcg ctatgaatgg
      721 gcgccgctgc ccgggacgcc cgaagcggcg ctttataccg attttcttcc ggtgcgcgac
      781 tggttggcat gagcggtgac ccgcgtcccg cggccgaccg gccggacgat ccgctcgccg
      841 agcggcgcat cggcggcgag caggtgtatg gcggcacgct gctcgacgtc cggcgcgatc
      901 gcgcaaggat gcccgacggc agcgacgcgg tgcgcgaata catcgtgcat ccgggcgccg
      961 tgctggtcgt gcccgtgcac gacgacgggc ggatgatcgt cgagcggcag ttccgctatc
     1021 cgcacaaccg cagcttcctc gagtttcccg cgggcaagct cgatccgggc gaatcgccgc
     1081 tcgaaagcgg cgtgcgcgag ctcgtcgagg aggccgggta tcgcgcgcag ctctggcttc
     1141 gtctgggcac gatccatccg gtgatttcgt attcgaccga ggcgatcgtc ctctatgcgg
     1201 cgcgcgggct cgtgcatgtc ggcgcacggc tcgacccggg tgaatttctc gagatcgtcg
     1261 agtgcaccga gtccgcactg tacgaggcga tcgacgccgg acgggtgacc gacgcgaaaa
     1321 ccatcgcggc gctggcgctt tattcgcggt ggagcgcggc gaccacgcga agcgtgcggc
     1381 tctccatcac cggccgcgtg cagggtgtcg gctttcgcga ttgggcgctg cgcgccgcag
     1441 cgcgggcgca attgcgcggc ttcgtgcgca atcgcggcga cggcagcgtc gaagcgcatc
     1501 tccagggcga acgccgcgcc tgcgaccggt tcatcgacga ttgccgggag ggtccgcgcg
     1561 catgccgcgt cgaacgcatc gaagtcgaac gtgcgccatt cgacgcatcg ctggccgact
     1621 tccgcgtcgc aagatcgaac tgaattcgcc gctggcgcgc gccgtcagtg cgccgtttct
     1681 tccgtccggc cctgtgcttg ccgggcatgc gcgtgcgtcg cgagcgcatg catgcccccg
     1741 gcgaggaacg cgatcagctc ttccggactc ggggtgaatg gcggcgggtt gccgctcgcc
     1801 ttgcagcgcg cgagcgcatc gtgctcgagc agcaggcggc aattgcgaaa gccggcggcg
     1861 agcgacgcat cgtcgacgcc cggcaatgca tggcgcagcg cgttcaggca ttcgcgatag
     1921 acgctgccga agtggcgcag catgatcgag tcggaagggt cgtgcccggc gaggcgagcg
     1981 acgaggcaaa ggtaattgcg ccacggcgac gcgccatccc ggtcgagcga cgcgaacggc
     2041 cggccgtacg cccacagcac gtcctcgacg gtgaaatcgc cgcgctcgcg cagcgcggca
     2101 agcgcgtcgt gctgcatggc gagcagttgc ccggcacgcc gcgcgagcgc ttcctcgatc
     2161 agctgctcct tgctgccgaa gtggtagttg agcagcgcga cgtgcgcctc ggcgcgcgaa
     2221 gcgatcgttc gatgcgtcag cggcttgaag ccgtactcgg cgatcagccc ttcaaccgcg
     2281 tcgagcagcc ggctgcgtgt atcgtccccg cggcggcgct ccgtgcgcgc gggtgtgtcc
     2341 gttccgatgg agccgactgc atgttccagg ggcatggcgg gatccccgca cgtgggtcgt
     2401 cgtggaaagt tcgcgaccat cgtagcgttg cgcaacgcga ggtcgcagca gacggaggat
     2461 cagattgatg taggacgccg ccgacacctc ggcgctcgac gccctgcaac gccttacgcg
     2521 acgccgaaca cgtccttcca cgcagcgtac gacgccgccg cggaccaggg cagcgcgagc
     2581 aggaggccaa tgccgctcgt cgcgagaccg atcatgagaa gcacgaacga cagcgcgccg
     2641 tagagcagca acggtgacgc gttgcgggcg aatgcggcgc cgctttccgc gaatgcggtg
     2701 cgaacgcctg cgccgtcgaa cagcaccgcg aacggcacga atgtcagcgg cagcgaaacg
     2761 acgacacccg ctgcatacgt cgcgacgagc gtcatgccgg aaatcgattc cgcgttgccc
     2821 gcgggcgcga gcatgttgaa accgcccacg gcattggcga cgagcgcttc ggcggcgaac
     2881 acgatgagcg ccgcggcgat gatcgccgcc tgcgcgcgcg ccggggccgc ggcgatcgca
     2941 accagatggc gcaggcgcgg gcggtcgccg cgatcggcgg cgaggctcgc atacagcagg
     3001 ccgcactcga tcagcggcag caggagctgc gcgagcacga tgccggcgat cggcacgagc
     3061 gtcagcgcga tgttcgcgac gatcacgacc agcgcgatcg cacagaagct ggccggcccg
     3121 cgcttccaca gccgcatcgc atcgcgatac cagtggagcg catgctgcaa aggcacggcg
     3181 cggatcgcgc gccgcggcgg ttcggacccg ccgctcggag cggggtaggc gttgccggga
     3241 atgcgtcgtg cccaggagtt ggaggatcgg gtcaggcggc ttcgccgagg tacgccgcac
     3301 gcaccttcgg gtcgtgcagc aaatcggcgg ccttgcctgc gagtgtaacc tcccccgatt
     3361 ccatcacgta gccgcggtcg ctcacgtcga gcgcgagctt cgcgttctgc tcgacgagca
     3421 ggattgtcac gccctcactg gccacggaaa ggatcgtctc gaagaccttc tgcaccatca
     3481 gcggcgcgag gcccatcgag ggctcgtcga gcaggagcag gcgcggccgc gacatcatgg
     3541 cccgcgcgat cgccagcatc tgctgctcgc cgccggacag cgtgccggcc gtttgccggc
     3601 gccgctcctt gagccgtgga aacaacgcga acacgcg
//
LOCUS       DASSVX010000108         9327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1470487, whole genome shotgun sequence.
ACCESSION   DASSVX010000108 DASSVX010000000
VERSION     DASSVX010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9327
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470487"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VFO53_04865"
     CDS             <1..803
                     /locus_tag="VFO53_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET8875641.1"
                     /translation="ESKLQNASLRFVEPPAANGWSRTTFARIERTNISGLVTQTAATG
                     LRMSSLDERNQWQYGAAFYVDQERPTGFAPANSRALYVDVERAWRRVDDLAAPTRGFI
                     VLAQAGVGIPGASTRGFERVILRSALWRPLGPRWSLTARAEAGAVIAGDRVDIPSELL
                     FRTGGDTSVRGYAFESLGLKQGDVVLPVRYYTVTSVEVTRWINDALGIATFVDAGNAF
                     DEASDFHLAVGYGIGARVKTPIGPFRLDVAYGEQSRQVRLHFSVGVAF"
     gene            811..4692
                     /locus_tag="VFO53_04870"
     CDS             811..4692
                     /locus_tag="VFO53_04870"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET8875642.1"
                     /translation="MTLPGNAATRPPAHRRGVWIALAIVALVVIAVVGVVGWGLSEHG
                     LPFIVDRIVARSGGRVSVEGASGSIAGTMRFRRVTWHGADATLVADDVVVDWNPGTLL
                     HKHLSIRGLGARHVDLAIKPSQGAPATPPADLRLPLAVDIDRLAIGEFDWHTGPRSGR
                     ISGLELGYSGDESAHHIRDLALVSEYGALRGSVDVGARAPLDVSGHATLDGDGAIKGA
                     HAEATLEGPLARLAVAMTGTYRDAALSLQALATPFSSTPFASATAELTGVDASTFDAS
                     LPRTRARLHLAFAPHGEGIAGTLDFVNEAPGPIDANAVPVAQLAAKFALDEAALRLDA
                     IDAMLGGGSAHGEARIALASAARSVHLALSIADLDLARIQSKLAATRLSGRVSADADR
                     ERQVVEGDIREKDMTLAFAAAIAGGRVDVSRFRASAGAGSLAGSAQVALNDSNDFTLR
                     ATMERLDPSRFAAVPRASIDGTINATGVVHPRWRASVDVGIAPSSRLEGVALSGTAKG
                     MVAPGVVSGLVADVALASVHLSASGAAGSPGDRLAFTLDAPQLGDIAALLPASVPHPV
                     AGSLHASGHLALAGSTAGGDIEWRAQALRAGDYRAATLSGRASLAPAAMTRATLDNRA
                     LAFDVDAGGLALAARAIDTLHASASGSLAHHHATLALGAADIATTLALDGSLRNADHP
                     ADATWSGTLASLDNHGAVPLHLDGTAALTLRSRYVRIQGARIGVADGRADIGDFIWNE
                     GRITTHGSFTGIPVTSAARLAGQKLPLDSTLVVGGDWSIAAAPRLSGSFSLAREHGDV
                     FADIPSGASTKREGIGVSELRIDGTFHDDALDARASFASARAGSASGSASIGSVSGAT
                     SGKIDRAAPLRLALRAELASLAVFQPWFGTVAAVDGRAELDVAASGTVGTPLWSGTLA
                     GSGLSVNAPQYGVNLADGRLQAHLAQDGIALDALRFRGGDGTFEASGLVALPGARARA
                     PTQVTWKAERFRVANRPDMRFVVDGNGTVALADKRLALRGSITVVEGHVEYEQSPTGK
                     LASDIVVEGETYVDRQSSPGNVPLALDVEVDLGSKLTFVGEGVDTRLAGRIRVTTDAN
                     GNVRANGTIRSVNGTYIAFGQKLTIDRGRLIFDGPPDNPALDVVALRKNLPVEAGVEL
                     SGTVKVPRVRITSNPPVPENEALAWLVTGQGLNGTGRVDYAALSAASAALLGRHGQPF
                     TAGVAHRLGLDDISLQSSGASTSGAQGTASQVVVFGKRISDRLSLGYEQGLSLASSAV
                     RLEYALSRQVTLRAEAGTTSGIAIVYRRNFR"
     gene            complement(4822..5619)
                     /locus_tag="VFO53_04875"
                     /pseudo
     CDS             complement(4822..5619)
                     /locus_tag="VFO53_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            6643..6939
                     /locus_tag="VFO53_04880"
     CDS             6643..6939
                     /locus_tag="VFO53_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr-rel-A system PqqD family peptide chaperone"
                     /protein_id="HET8875643.1"
                     /translation="MSRDVIWRALDAQAIDSRAWNGESVLYNDVTGSTHQLSVLGSAV
                     MATLLEHPSGIPLTALVRRIADTAGDVADDELRAAVEHTLAALAELRLAASVST"
     gene            6936..7847
                     /locus_tag="VFO53_04885"
     CDS             6936..7847
                     /locus_tag="VFO53_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HprK-related kinase A"
                     /protein_id="HET8875644.1"
                     /translation="MILGELPAGELRSRLAGSGLRLRIGPVVAEILSSFRTVESALAL
                     HYAGHEVVPATDFADFHVAVEPPPGVRRWVAPQAYFRFDDHSAFLPLPAAQAFPLLEW
                     GLNWCVSVHCHQYLIIHAAVVERGGRALVLPAPPGSGKSTLCAALVARGWRLLSDELA
                     LVELSGRMLMPMPRPISLKNRAIDVIRTFWPEARIGAVVPDTLKGSVAHVRPPIDSVR
                     RAHEPATPGWIVLPRFEAGAITQLRPVSKCEAFMRLVDSAFNYDIHGRAGFAALAEVV
                     DASTCRTFTYGGALDEAANVFDDLAKA"
     gene            7844..8923
                     /locus_tag="VFO53_04890"
     CDS             7844..8923
                     /locus_tag="VFO53_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET8875645.1"
                     /translation="MNAAPSGLIAGALRDPVRLCESGVRWEALIRSTREADLLGTLAF
                     RAQACAKLACIPPAPRAHLIAAGHRYRAQQAAVRREIAEITRAVQHLDVPIVLLKGAA
                     YLLAELPPARGRFFSDIDILVPKARLPEVESALMLAGFATTHLHPYDQRYYRRWMHEL
                     PPMRHVKRLSLLDVHHTLVPETAQVALDPEQLFRAAVPLAGMPGLFVLSPTDMVLHSA
                     THLFHNEELSHGLRDLVDLDALLDHFAREHSAFWPALVKRAAELGLQRPLYYALRFAP
                     RLLGTPVPGDVVASVARYAPPAAIGLLMDRLLAAALEPAYGHASTRWARRALYVRGHW
                     LKMPLPLLAWHLTVKALRREQTPPA"
     gene            complement(8940..>9327)
                     /locus_tag="VFO53_04895"
     CDS             complement(8940..>9327)
                     /locus_tag="VFO53_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET8875646.1"
                     /translation="EGQQVLITGYPLGAVLGAFPVTHRGMISAITPIAIPQARAAQLA
                     PAVIRRLSSGSFTVFQLDATAYPGNSGSPLYDPLTGEVFGIVNMVFVKSTKESALTQP
                     SGITYAIPASHLKALIDTRQRSATKR"
BASE COUNT         1401 a         3254 c         3160 g         1512 t
ORIGIN      
        1 tcgaatccaa gctgcagaat gcgtcgctgc gctttgtcga gccgccggcg gcaaacggct
       61 ggtcgcgcac gacgttcgcg cgcatcgagc gcaccaacat aagcggcctc gtcacgcaga
      121 cggcggcaac cggattgcgc atgagctcgc tcgacgagcg caatcaatgg caatacggcg
      181 cggcgttcta cgtcgaccag gaacggccaa cgggattcgc gcccgcgaat tcgcgcgcac
      241 tctacgtcga cgtcgagcgt gcgtggcggc gcgtcgacga tctcgccgcg ccgacccgcg
      301 gattcatcgt gctcgcgcaa gcgggcgtcg gcattcccgg cgcctcgacg cgtggattcg
      361 agcgtgtcat cttgcgctcg gcgctttggc ggccgctcgg tccccgctgg tcgctgaccg
      421 cccgcgccga agccggcgcg gtgatcgccg gcgatcgcgt ggacattccg tcggaactgc
      481 tgtttcgcac gggcggcgac accagcgtgc gcggctacgc gttcgaaagc ctcgggctca
      541 agcagggcga cgtcgttctt ccagtgcgct actacacggt cacgagcgtc gaagtcacac
      601 gctggatcaa cgatgcgttg ggcatcgcga cgttcgtcga cgccggcaat gcgttcgacg
      661 aggcgtccga tttccacctg gccgtcggct acggcatcgg cgcgcgcgtg aagacgccga
      721 tcggcccgtt ccgtctcgac gtggcgtacg gcgagcagtc gcggcaggtg cgattgcact
      781 tttcggtcgg tgtcgcgttc tgacgcaacg atgactctgc caggtaacgc cgcgacccgg
      841 ccgcccgcgc atcgccgcgg tgtctggatc gcgcttgcga ttgtcgcgct cgtcgtcatt
      901 gcggtggtcg gcgtcgtcgg ctggggcctg agcgagcacg gcctgccgtt catcgtcgat
      961 cgcatcgtcg cgcgctcggg cgggcgcgtc agcgtcgaag gcgcgagcgg atcgatcgcc
     1021 ggcacgatgc gctttcgccg cgtcacctgg catggcgccg acgcaacgct cgttgcagac
     1081 gacgtcgtcg tcgactggaa tcccggcacg ctgctgcaca agcacctgtc gattcgcggt
     1141 ctcggcgcgc gccacgtcga tcttgcgatc aagccgtcgc agggcgcgcc ggcaacgccg
     1201 cccgcggatc tgcgcttgcc gctggccgtc gacatcgacc ggctcgcgat cggtgaattc
     1261 gactggcaca ccggtccgcg atcgggccgc atttcggggc tcgagcttgg ctattcgggc
     1321 gacgaaagcg cgcatcacat tcgcgacctc gcgctcgttt ccgaatatgg cgcgctgcgc
     1381 gggagcgtcg acgtcggcgc gcgcgcaccg ctcgacgtgt cgggtcatgc aacgctcgac
     1441 ggcgacgggg ccatcaaggg cgcgcacgcc gaggccacgc tcgagggccc gctcgcacgt
     1501 cttgccgtcg cgatgacggg cacgtaccgc gacgcggcgc tttcgctgca ggcgctcgcg
     1561 acgccgtttt cctcgacgcc gttcgcctcg gccacggcgg aactgaccgg cgtcgacgcg
     1621 tcgacgttcg acgcgtcgct gccccgcacc cgtgcacgcc tgcatctcgc ctttgcgccg
     1681 cacggcgaag gcatcgccgg cacgctcgac ttcgtcaacg aagcgccggg ccccatcgac
     1741 gcgaacgcgg ttccggtcgc gcagctcgcg gcgaaattcg cgctcgacga agcggcgctg
     1801 cggctcgatg cgatcgatgc gatgctgggc ggcggcagcg cccatggcga ggcgcgcatc
     1861 gcgctcgcga gtgccgcgcg ctccgtccat ctcgcgctgt cgatcgccga cctcgacctt
     1921 gcgcgcattc aatcgaagct cgccgccacc cggctgtcgg gacgcgtcag cgccgacgcc
     1981 gaccgcgagc ggcaagtggt cgaaggcgac attcgcgaaa aggacatgac actcgcgttt
     2041 gccgccgcga tcgcaggcgg ccgcgtcgac gtctcgcggt ttcgtgcaag tgccggtgca
     2101 ggctcgcttg ccggctcggc gcaagtcgcg ttgaacgaca gcaacgactt cacgttgcgc
     2161 gcgacgatgg agcgcctcga tccgtcgcgt ttcgccgcgg ttccgcgcgc gtcgatcgat
     2221 ggaacgatca atgccacggg cgtggtgcat ccgcgctggc gcgcgtcggt cgacgtcggc
     2281 atcgcgcctt ccagccggct cgaaggtgtc gcgctttccg gtaccgcgaa gggcatggtc
     2341 gcgccgggcg tggtgtcggg actcgtcgcg gacgtggcgc tcgcgtcggt gcatctgagc
     2401 gccagcggcg cggccggctc gcccggcgac cgcctcgcgt tcacgctcga cgcgccgcaa
     2461 ctcggcgata tcgcggcact ccttccggcg agcgtgccgc atccggtcgc aggctcgctg
     2521 catgcaagcg ggcatctcgc actcgccggc agcaccgccg gcggcgacat cgagtggcgc
     2581 gcgcaagcgc tgcgtgcagg cgactaccgc gcggcgacgc tttccggacg cgcgtcgctc
     2641 gcccccgcag cgatgacccg cgcaacgctc gacaaccgcg cgcttgcctt cgacgtcgac
     2701 gccggcgggc tcgcgctggc ggcgcgagcc atcgacacgc tgcacgcgag tgccagcggc
     2761 agcctcgcgc accatcacgc caccctggca cttggcgctg ccgacatcgc cacgacgctc
     2821 gcgctcgacg gatcgctgcg caacgccgat catccggcgg acgccacgtg gagcggcacg
     2881 ctcgcctcgc tcgacaatca cggtgcggtt ccattgcacc tcgacggcac cgcggcgctg
     2941 acgttacgca gccgttacgt gcgcatccag ggcgcgcgca tcggcgtcgc cgatggccgc
     3001 gccgacatcg gcgatttcat ctggaacgaa gggcgcatca cgacgcatgg ctcgttcacc
     3061 ggcattccgg tcacgtcggc cgcgcgcctt gccggacaga agctgccgct cgattcgacg
     3121 ctggtcgtgg gtggcgactg gtcgatcgcc gctgcacccc gtctttcggg aagcttttcg
     3181 cttgcacgcg agcacggcga cgtgttcgcc gacatcccgt cgggcgcatc gacgaagcgc
     3241 gagggaatcg gcgtcagcga gctgcgcatc gacgggacgt ttcacgacga cgcgctcgac
     3301 gcgcgcgcat cgtttgcgtc cgcgagggcg ggcagcgcga gcggcagtgc gagcatcggc
     3361 agcgtgagcg gcgccacgtc gggcaagatc gatcgtgcgg cgccgctgcg gctcgcgttg
     3421 cgcgccgagc tcgcgtcgct cgcggtgttc cagccctggt tcgggacggt ggcggcagtc
     3481 gatggccgcg cggagctcga cgtcgccgcc agcggcacgg tcggcacgcc gctgtggtcg
     3541 ggcacgctcg cgggcagtgg cctgtcggtc aacgcgccgc agtatggcgt caatctcgcc
     3601 gacggccggc tgcaggcgca tcttgcgcag gacggcatcg cgctcgacgc actgcgcttc
     3661 cgcggcggcg acggcacgtt cgaagcaagc ggtctcgtcg cattgccggg cgcaagggcg
     3721 cgcgcgccga cacaggtgac gtggaaagcc gagcgcttcc gcgtcgcgaa ccgtcccgac
     3781 atgcgcttcg tcgtcgacgg caacggcacc gttgcgctgg cggacaagcg cctcgcgctg
     3841 cgcggcagca tcaccgtggt cgaaggccat gtcgaatacg agcagtcgcc gacgggcaag
     3901 ctcgcctcgg acatcgtcgt cgagggcgaa acgtacgtcg atcgccagtc gtcgccgggc
     3961 aacgtgccgc ttgcgctcga cgtcgaggtc gacctcgggt cgaagctcac gttcgtcggt
     4021 gaaggcgtgg atacgcggct cgccggccgc attcgcgtca ccaccgacgc gaacggcaac
     4081 gtgcgcgcga acggcacgat tcgctcggtg aacggcacgt acatcgcatt cgggcagaag
     4141 ctcacgatcg atcgcggccg cctcatcttc gacggcccgc ccgacaatcc ggcactcgac
     4201 gtcgtcgcgt tgcgcaagaa tctgccggtc gaagcgggcg tcgagctgag cggcacggtc
     4261 aaggtgccgc gggtgaggat cacgtcgaat ccgccggtgc cggagaacga ggcgctcgcg
     4321 tggctcgtca ccggacaggg cctgaacggc accggtcgcg tcgactacgc cgcgctgtcg
     4381 gcggcgtcgg cggcgctgct cggccgccat ggccagccgt tcaccgccgg cgttgcacat
     4441 cgccttggcc tcgacgacat ctcgctgcag agcagcggcg cgagcacgag cggcgcgcag
     4501 ggcacggcga gccaggtcgt cgtgttcggc aagcgcatca gcgaccggct ctcgctcggc
     4561 tacgagcagg gactgtcgct cgcgagcagc gcggtgcggc tcgaatacgc gctgtcgcgg
     4621 caggtgacgc tgcgcgcgga agcgggtacc acgagcggca tcgcgatcgt gtatcggcgc
     4681 aattttcgtt agggcgtgag tgccttcacc cggcgcgcgc gccatcgcgc tccgcgggct
     4741 cacgtcgtac agcacgttcg cgaggatttc ataaatcgcg tagtagctga gctcgaggcc
     4801 gcgcagaccc ggaaggtaat cgccgatatc ggtctcatcg agcggcggcg caatgccgat
     4861 cggcgcggcg atcacccgga tgcctgccgc ttcgaattcc ttgcgcgagc gcggaaaatc
     4921 gaaactgtgg ccgacgagga tgacgcgctg gacgccgctt gccgcaagga tcttcgccga
     4981 gtcgacggcg ttctcgtgcg tattgtgcga tcgcgtttcg acccagcgca ccggcacgcc
     5041 gagctcgttc acgagcacgt tgcgcatcag gacggcttcg ggaggcgcgc cgcgcacgcc
     5101 gccgcccgac acgagaaccg gaaggcccgt cacacgcgca atacgcgcgc cgtagcgaag
     5161 gcgctcgagc gtgatccggc tgaccgtggc gccaccgtat tccggcgcgt agcggcgcgt
     5221 gccgccgcca agaatcacga tggcctgcgc gctttcggcc gcgtggacga catcgagcgc
     5281 cggtgcctga tcgagactgc ggacgagaaa ttcgccgatg accggtgtcg cgagcagcca
     5341 cagcgcgagg acacccgtca gcgcgattcg tcgcccggcg cgcggatgcc tgccggcgag
     5401 cgcgaggccg gcgaatgcga gcagcagcgg agcgaccggg ggcagcgcga cgagcttgac
     5461 gacctgcttc agccagaaaa gtgggggcat cgcttcgatt ccgacgcgag ttcgcgcgat
     5521 cgtccgcgcc ggcacggccg gcagccgcgt cgcgccgatg agagcataat cgggggaaat
     5581 ccgccgcgtg cgaaacattc gccgcgcacg gcagtccatc cgagcggtac ccgcccggtg
     5641 gtcgcggcca tgagcatacc gtgcaacggc tgtcggcgtg ctttacaact acagcggggt
     5701 accctacccc ttcattcgca accttttgtt ttttgtcgcc ttggcgggca ctcactccaa
     5761 tggaacgacg attgcttcac tcagacgcaa atggtactcc cccctgaact cccacccggt
     5821 gcaccgggtg tcgagccgcg tccgtcgggc gcgcgccgcc gtcttttgca aaccgggctg
     5881 gcggtcggcc ccgtattgat gaccgtcgca agccgcccgg tgctcggatc gacggcatgc
     5941 acgacgacgt ccgcatggat gtcgatgacg ggaagcacgg cgggcaaggc cgaagtgtgc
     6001 agcgggcttt cgccagaagc ctggaagggc aacgccacgc agtggccgag cccttatgta
     6061 ggcgccgtac tcggcgcacc ggtcgccatg gatcccggag cggtgagcgc gcccgtgccg
     6121 ggagggtccg gcggcgggcc aatgctcggc gatcccatca gcgctcctgg cgtcgacggc
     6181 agcggcggcc tgaaatgggg cacgcgtctt tcattgggtg gcacgtcttc caccagcacg
     6241 accaccacat cggtttcgcc accggggggc accgaattcg aaaagccgac gcggtaccac
     6301 tgccccacga ccggattcgc cggcaacgta ttcgggacgc gcacgatgct cgaagtgctc
     6361 gatgtctcgc aggccggtct cgacggcgtc ggccgctaca tggtcgccgc cctcctgaat
     6421 gcgcgctcgg gccgcacgcc ggttctcgac gaaacgggcg tgcgcaccat gtggaacgac
     6481 ctcgtcaatc acggctatta cgagccgacc gccggcatca agtggaccgg cgccgagatc
     6541 atcgcctacc tgcagtcgac gatgggttag cgcccgcggc cacgccgcgc gcatgccacc
     6601 caccgtacgt ggcttcgttc tgaagcgctt gctcgccgct gcatgagtcg cgatgtcatc
     6661 tggcgggcgc tggatgcgca agcgatcgat tcgcgcgcat ggaacggcga atccgtcctc
     6721 tataatgacg tgaccggcag cacgcaccaa ttgagcgtgc tcggcagtgc ggtcatggcc
     6781 acgctcctcg aacatccttc gggtatcccg ctcacggcgc tggtacgccg catcgccgat
     6841 accgcaggcg acgtcgccga cgacgagctg cgcgcggccg tcgaacatac gctggcagcg
     6901 ctcgccgagc tccgtctggc ggcttccgtc tcgacttgat tctcggggag cttcccgccg
     6961 gcgaactgcg cagccggctg gccggatcgg ggctgcgttt gcgcatcggt cccgtcgtcg
     7021 ccgagatcct gtcatcgttt cgcacggtcg aatcggcact cgcgctgcac tacgcgggac
     7081 acgaggtcgt accggccacc gacttcgccg attttcacgt ggcggtcgag ccgccgccgg
     7141 gtgtccggcg atgggtcgcc ccgcaggcgt acttccggtt cgacgaccat tcggcatttc
     7201 tgccgcttcc ggccgcgcag gcgtttccgc tgctcgaatg gggattgaac tggtgcgtct
     7261 ccgtccattg tcaccagtac ctgatcatcc acgcggcggt cgtcgagcgc gggggccgcg
     7321 cgctcgtcct tcccgcacct ccgggctccg gcaaaagcac gctgtgtgcc gcgctcgttg
     7381 cgcgcggctg gcggctcctg tccgacgagc ttgcgctcgt cgagctttcc ggaagaatgc
     7441 tgatgccgat gccgcggccg atcagtctca agaaccgcgc gatcgacgtg atcagaacgt
     7501 tctggccgga agcccgtatc ggcgcagtcg tgcccgacac gctcaagggt tccgtcgcac
     7561 acgtgcgtcc gccgatcgac agcgtgcgcc gcgcgcacga gccagccacg cccggctgga
     7621 tcgttcttcc ccgtttcgaa gcgggagcca tcacccaact gcggccggtg tcgaagtgtg
     7681 aagcgttcat gcggctcgtc gacagcgcgt tcaattacga cattcacggt cgcgcgggat
     7741 ttgcggcact cgccgaggtg gtcgacgcca gcacgtgccg gacgtttact tacggcggcg
     7801 cgctcgatga agccgccaac gtcttcgacg atctcgcgaa ggcgtgaacg cggctccgag
     7861 cggtctcatt gccggcgcgc tgcgcgaccc ggtgcggctt tgcgaaagcg gcgtgcgctg
     7921 ggaagcgctg attcgatcca cccgggaagc cgacctcctc ggcacccttg ccttccgtgc
     7981 gcaagcctgc gcgaagctgg cctgcattcc gcccgcaccg cgtgcgcacc tgattgcggc
     8041 cggacaccga taccgcgcgc agcaggctgc ggtgcgccgc gaaattgcgg aaatcacccg
     8101 tgcggtgcag cacctcgatg taccgatcgt tctgctgaaa ggcgcggcct acctgctcgc
     8161 cgagcttccg cccgcccggg gacggttctt ttccgacatc gacattctcg ttccgaaagc
     8221 gcgcctgccg gaagtcgagt cggcgctgat gcttgccggt ttcgcgacga cgcacctgca
     8281 tccgtacgac cagcgctact accgccgctg gatgcacgag ctgccgccga tgcgccacgt
     8341 gaaacggttg tcgctcctcg acgtgcacca cacgctcgtc cccgagacgg cgcaggtcgc
     8401 gctcgacccc gaacagctct ttcgcgcagc ggtgccgctc gcgggcatgc cgggcctgtt
     8461 cgttctgtca ccgaccgaca tggtcctgca cagcgcgacg cacctcttcc ataacgagga
     8521 gctttcgcac ggcctgcgcg atctcgtcga cctcgacgcg ctgctcgatc atttcgcacg
     8581 cgagcacagc gcattctggc cggcgctcgt gaagcgcgca gccgagctcg gcctgcaacg
     8641 gccgctgtac tacgcgctgc ggttcgcgcc acggctgctc ggcacgccgg taccgggcga
     8701 cgtcgtcgcg agcgtcgcgc gttacgcgcc gccagcggcc atcgggcttc tgatggaccg
     8761 gctgctcgcc gcggcactcg agcccgcgta cgggcacgcc agcacgcgct gggcaaggcg
     8821 cgcactgtac gtgcgcgggc actggctgaa gatgccgctt ccactgctcg cgtggcacct
     8881 gacggtcaag gcgctgcgcc gcgaacagac gccgccggca tgacgcacgc aacacgcgcc
     8941 tatcgcttcg ttgccgatcg ttgcctggtg tcgatcagcg ccttcaggtg gctcgcaggt
     9001 atggcgtagg tgatgccgct cggctgcgtc agagccgatt ccttcgtgct cttgacgaaa
     9061 accatgttga cgatcccgaa cacttcgccg gtcagcggat cgtacagcgg acttccgctg
     9121 ttgccgggat acgcggtcgc atcgagctgg aacaccgtga agctgccgct gctgaggcgg
     9181 cggatcacgg cgggcgcgag ctgcgctgca cgcgcctgcg ggattgcgat gggcgtgatc
     9241 gccgaaatca tgccgcgatg cgtcacgggg aacgcgccga gcaccgcgcc cagcgggtag
     9301 cccgtgatga ggacctgctg accctcg
//
LOCUS       DASSVX010000109         5905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_970513, whole genome shotgun sequence.
ACCESSION   DASSVX010000109 DASSVX010000000
VERSION     DASSVX010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970513"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3709)
                     /locus_tag="VFO53_04900"
     CDS             complement(<1..3709)
                     /locus_tag="VFO53_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HET8875647.1"
                     /translation="MKPAFRHLLHAGPSRFEQDDQEPLWSELFSPERLEQHARSLAAA
                     QPVSKRPPRWRSFANRNAENADVLREAYAAISDAVKSKRLITPAADWLVNNFHVVEDQ
                     LRDIRTLLPRRFYARLPALDAGPLAGLPRVYGIAWAFVAHTDSRFDPDLLLRFVAAYQ
                     KDDPLELAELWALPTVLRIVMIENARRLAVRIVASMKGREQADRYANALTGLATDTVD
                     DVSLVSAGTASGWSRAFTVQLFQRLRHRDAAAAVALQELSQRLAAEGTSAEAVVQMEI
                     GRQAAADLSVRNLITSMRLLSAYEWPDFVEAASLVNHALSRHPLFARMDFITRDRYRS
                     AIEDLARRSQRTELDVADAVVAKTAAMSDERESRVAELGFWLIGDGRTVFEREIGYRR
                     PHWRRVLQRQANHALPIYAGTIIVVAAGILALLLTSAQVQGVGQALLWIYAALTLAPA
                     SEIALLLVNRLVADHVTPRHLPRLRLPEGLDDATRTFVVVPTMIGSEQSIIANVRMLE
                     AHYLSNGVNTGEIYFALLTDWLDASSERADGDDELLRTASDAIHALNTRYGAAPSGTQ
                     RFYLFHRQRKWNPAQGTWMGWERKRGKLHEFNRLLRGANDTSFMLDLPHGARPPAGVR
                     FVMTLDADTIMPFGVATELVGTLLHPLNRPQIDAASGTIVEGYAILQPRVTPTLPTRQ
                     SSSRFQEWFSGHCGVDPYTFHVSDVYQDLFGRGSFTGKGMYEVDAFEAALRGRMPPDA
                     ILSHDLLEGSYARCAFVSDVELFEEFPAHTEVAAARSHRWARGDWQLLPWIIGRRSRQ
                     LSPIAQWKMLDNLRRTLVAPSLFLLLAIAWLAEGAGAQRFALLALATFLLPALLALVD
                     QLVPRPNTDRGNWLRMLRDDALQGFGRALFQLAMLPRDCVLMIDAIVRTLGRLVVRQR
                     LLEWNSAAQVEASASMTLASFVRTQDAALAITIAVLFALLITSSDALTIAAPFLVLWL
                     AAPLFAWRASQPVIARDIEAPTAADAAQLRLIARQTWQYFADLAGPDENFLPPDNLQV
                     DPARVIAHRTSPTNIGMYLVATVAAHDFGWVGLPEMCERVEACLATLAKLPRYRGHFF
                     NWIETTTLRSLEPRYVSTVDSGNLAGCLITLSSAARMSLARPAIDRTAWRGVADDVAL
                     LRRAVERADRKRPSTSVNVVQLQDALAALDALLAKPQAGRVLRWRELERLASDVLDMA
                     RVIAAEGAHPSLTDVEISAGVLHDGIARRHAE"
     gene            4002..4943
                     /gene="hemC"
                     /locus_tag="VFO53_04905"
     CDS             4002..4943
                     /gene="hemC"
                     /locus_tag="VFO53_04905"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET8875648.1"
                     /translation="MTAPFAPRRLTIATRESALALWQAEHVRAQLTEQFRGTTVELLG
                     ITTQGDRIVDRPLATIGGKGLFIKELETALREGRADVAVHSLKDVPMDVPEGFVLAAI
                     SSREDARDAFVSNRFGALAELPDGAVVGTSSLRREAQLRERYPRLSIASLRGNVHTRL
                     RKLDEGGYDAIILAAAGLKRLGLGTRIRSLLDPDESLPAPGQGALAIECRADRTDLRT
                     ALGYIADRSTTLATAAERAFSRALGGSCHTPLAAYAEWEEDRLWLRGLIATRDGGDVM
                     RGERESAVVDIDGARALGGALADEFLAQGAHRFASSA"
     gene            4966..5787
                     /locus_tag="VFO53_04910"
     CDS             4966..5787
                     /locus_tag="VFO53_04910"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET8875649.1"
                     /translation="MTTGVHSLSPPQRERGPLDGIGIVVTRPLRQAAVLAARIAALGG
                     MPIIWPAIVILPPPDRAPLTRAHAQLAQYDIAIFVSANAVEFGAPPLGAWPATIVTYA
                     PGPGTAEALAAAGIAGALQPTTSFDSEGLLELPTLADVTGKRVVIFRGEGGREFLGST
                     LRSRGATVDHVPCYRRVAPQSGADGLVEAMREGRAHALTLTSSEGLDNLVAAVGEAGR
                     SLMAQLPVFAAHPRIAERAREHGLRAVETAGGDAGLLAGVLDWFAGPPRSAARSA"
BASE COUNT         1003 a         1976 c         1999 g          927 t
ORIGIN      
        1 attccgcatg acgccgcgca atgccgtcat gcagaacgcc ggcgcttatc tcgacatcag
       61 tgaggctcgg atgcgcgccc tctgcggcga tgacgcgcgc catgtcgagc acgtcgctcg
      121 caagtcgctc gagctcgcgc cagcgaagga cacgccccgc ctgcggcttt gcgagcagcg
      181 catcgagcgc ggcaagcgcg tcctgcaact gcacgacatt gacgctcgtc gatgggcgct
      241 tgcgatcggc gcgctcgacg gcgcgccgta gcaacgcgac atcgtcggca acgccgcgcc
      301 aggcggtacg gtcgatcgcg ggccgcgcaa gcgacatgcg tgcagccgat gacagcgtga
      361 tcaggcagcc ggcgagattg ccgctgtcga cggtggacac gtaccgaggc tcgagcgagc
      421 gcagcgtcgt cgtctcgatc cagttgaaaa aatgaccgcg ataacgcggc agcttcgcga
      481 gcgtcgcaag acacgcttcc acacgctcgc acatctcggg caggccgacc cagccgaaat
      541 cgtgcgcagc gaccgtagcc acgaggtaca tgccgatatt cgtcggcgac gttcgatggg
      601 cgatgacccg cgccggatcg acctgcaggt tgtcgggcgg caggaagttc tcgtcggggc
      661 ccgcgaggtc ggcgaagtat tgccacgtct gccgcgcgat cagccgcagc tgcgcagcgt
      721 cggcggctgt cggtgcctcg atgtcgcgtg cgatcaccgg ctggcttgcg cgccacgcga
      781 agagcggtgc ggcgagccac agcaccagga agggcgcggc aatcgtcagc gcgtccgaac
      841 tcgtgatgag caatgcgaac aacacggcaa tggtgatcgc cagggccgcg tcttgcgtgc
      901 ggacaaagct cgcgagggtc atcgatgcgc tcgcctcgac ctgcgcggcc gagttccatt
      961 cgagcagccg ctgccgtacg acgagccggc cgagcgtgcg gacgatcgcg tcgatcatca
     1021 gcacacagtc gcgcggcagc attgcgagct ggaacagcgc gcggccaaaa ccttgcagcg
     1081 cgtcgtcgcg cagcatccgc agccagttgc cccgatcggt gttgggacgc ggcacgagtt
     1141 gatcgacgag tgcaagcagc gccggcagga gaaacgtggc gagcgcgagc agcgcaaaac
     1201 gctgcgcacc cgcaccctcg gcgagccacg cgatcgcgag cagcaggaaa aggcttggcg
     1261 cgacgagcgt gcggcgcagg ttgtcgagca tcttccactg cgcgatcggc gacagttggc
     1321 ggctgcggcg gccgatgatc catggcaaca gttgccagtc gccgcgcgcc catcggtggc
     1381 tgcgcgccgc cgcaacttcg gtgtgcgccg ggaattcctc gaacaactcg acgtcgctga
     1441 cgaatgcgca gcgagcgtag cttccctcga gcaggtcgtg gctcaaaatc gcgtcgggcg
     1501 gcatcctgcc gcgcagcgct gcctcgaacg cgtcgacctc gtacattccc ttgccggtga
     1561 acgagccgcg gccgaacaga tcctggtaga cgtcggagac gtggaacgtg tacggatcga
     1621 caccgcagtg gccggagaac cattcctgga atcgactgct cgattgccgc gtgggcagcg
     1681 tcggtgtcac gcgcggctgc aggatcgcgt agccctcgac gatcgtcccg ctcgccgcat
     1741 cgatttgcgg gcggttgagc gggtgcagca gcgtaccgac gagctcggtc gcgacaccga
     1801 acggcatgat cgtgtcggcg tcgagggtca tgacgaatcg cacacctgcc ggcggccgcg
     1861 caccgtgcgg caggtcgagc atgaacgacg tgtcgtttgc gccgcgcagc agccggttga
     1921 attcgtgcag cttgccgcgc ttgcgctccc agcccatcca cgtgccttgc gccggattcc
     1981 acttgcgctg gcggtggaaa aggtagaagc gctgcgtgcc ggaaggcgca gcgccgtaac
     2041 gcgtgttcag cgcgtgaatc gcatcgctgg ccgttcgcaa tagctcgtca tcgccgtcgg
     2101 cacgctcgga cgatgcatcg agccagtcgg tcagcagcgc gaagtaaatc tccccggtgt
     2161 tgacgccatt cgacaggtaa tgcgcctcga gcatccgcac gttcgcgatg atcgactgct
     2221 cgctgccgat catcgtcggc acgacgacga acgtacgcgt cgcgtcatcc agtccttccg
     2281 gcaatcgcaa ccgcggcaga tggcgcgggg taacgtgatc ggcaacgagc cggttgacga
     2341 gcaggagcgc aatctccgac gccggcgcaa gcgtcaacgc cgcgtagatc cacagcaatg
     2401 cctgcccgac accttgaacc tgcgccgacg tcaaaagcaa cgcgaggatg ccggcggcaa
     2461 cgacgatgat cgtgcccgca taaatcggca gtgcatggtt agcctgtcgt tgcagcacgc
     2521 gccgccaatg gggccgccga taaccgatct cgcgctcgaa cacggtcctg ccatcgccga
     2581 tcagccagaa tccgagctcc gcgacgcgcg actcgcgctc gtcgctcatc gccgccgtct
     2641 tcgcaacaac agcgtccgca acgtcgagct ccgttcgctg cgatcgccgc gcgagatcct
     2701 cgatcgcgct tcgataacgg tcgcgggtga tgaaatccat tcgcgcgaac agcgggtggc
     2761 gcgacagcgc gtgattgacg aggcttgccg cctcgacgaa atcgggccac tcgtacgcgg
     2821 acagcaaccg catgctcgtg atcaggttgc gcactgacaa atcggcggcc gcctggcgac
     2881 cgatctccat ctgcacgacg gcctcggcgc tcgttccctc ggccgcaagc cgctgcgaca
     2941 gctcctgcag cgcgaccgct gcggcggcgt cgcggtggcg cagtcgctgg aacaactgga
     3001 cggtgaaggc gcgcgaccag ccggacgccg tgccggcgct gacgagcgag acgtcgtcga
     3061 cggtgtcggt cgcaagaccg gtcaacgcat tcgcgtatcg atcggcctgc tcgcggcctt
     3121 tcatcgacgc gacgatgcgc acggcgagcc gccgcgcgtt ctcgatcatg acgatccgca
     3181 gtacggtcgg cagcgcccac aattcggcga gctcgagcgg atcgtctttc tgatacgccg
     3241 cgacgaagcg cagcaggagg tcaggatcga atcggctgtc cgtatgcgcg acgaatgccc
     3301 acgcgatgcc atagacgcgc ggcaatccgg caagcggccc cgcatcgagg gcggggagcc
     3361 gcgcatagaa tcgtcgtggc agcagcgtgc ggatgtcccg gagctgatcc tcgacgacgt
     3421 ggaagttgtt gacgagccag tcggcggccg gtgtgatcaa tcgcttggac ttgacggcat
     3481 cgctgatcgc cgcgtaagcc tcgcgcagca cgtcggcgtt ctcggcattg cggttggcga
     3541 acgatcgcca gcggggcgga cgcttactga cgggctgcgc cgcggcgagc gatcgggcgt
     3601 gctgctcgag cctttcggga ctgaagagct ccgaccacag cggctcctgg tcgtcctgct
     3661 cgaagcggct cggaccagcg tgcagcagat gtcggaaggc gggcttcatt gcggatcggc
     3721 gttacgacga acgacgcggc accgatacag cgtccggtga cggcatgatg atcgggctcc
     3781 gcttgcgcgc cgcgcggctg cagcgtttga tgacaacgat cgatcgtgcg cggagacgct
     3841 cggtgggttc gattgtacga cgcaacgctg cgaccttgcc gcgaatgacg ccgccgccga
     3901 atccggcctg cggtgcccga cctgctaata tggccgcctc gccgcaacgc gcgttttccg
     3961 aatttgggtc ctttcccccg cgtcgccacg gcacggcggc aatgacggca ccctttgcgc
     4021 cgcgccggct gacgatcgcc acgcgcgaat ccgcgcttgc gctctggcag gccgagcacg
     4081 tccgcgcgca gttgaccgag cagttccgcg gcacgacggt cgagctcctc ggcatcacga
     4141 cgcaaggcga tcgcatcgtc gatcggccgc tcgcgacgat tggcggcaag ggacttttca
     4201 tcaaggagct cgagacggcg cttcgcgaag gacgcgccga cgtcgccgtt cattcgctga
     4261 aagacgtgcc gatggatgtc cccgagggct tcgtgctggc ggcgatctcg tcgcgcgaag
     4321 acgcacggga cgcattcgtc tcgaatcgat ttggcgcact cgcggagctt cccgatggcg
     4381 ccgtcgtggg aacgtcgagc ctgcgccgcg aggcgcaact gcgcgagcgc tatccgcgcc
     4441 tttcgatcgc atcgctgcgt ggcaacgtgc acacgcgctt gcggaagctc gatgaaggcg
     4501 gatacgacgc gatcatcctt gcggcagcgg gactcaagcg gctcggcctc ggcacgcgga
     4561 ttcgctcgct gctcgatccg gacgaaagcc tgccggcgcc agggcagggc gcgctcgcca
     4621 tcgaatgtcg cgccgatcgc accgacttgc gaacagcact tggctatatc gcggatcgat
     4681 cgacgacgct ggcaaccgca gccgagcgcg cgttctcgcg cgcgctgggc gggagctgcc
     4741 acacgccgct cgcggcgtac gccgaatggg aagaggaccg gttgtggctg cgcggcctca
     4801 tcgcgacgcg cgatggcggc gacgtgatgc gcggcgagcg cgaaagcgcg gtcgtcgaca
     4861 tcgacggcgc acgcgcactc ggaggcgcac tggctgacga gttcctcgcg caaggcgcgc
     4921 accggttcgc atcttccgca tgaacgagaa cgcgagcatc gtcgaatgac caccggcgtt
     4981 cattcgctgt cgccgccgca gcgcgagcgc ggtccgctcg atggcatcgg catcgtcgtc
     5041 acgcgtccgc tgcggcaggc agccgtgctc gccgcgcgaa tcgcagcgct cggcggcatg
     5101 ccaatcatct ggccggcgat cgtcatcctg cccccacctg atcgcgcgcc actgacgcga
     5161 gcgcatgcgc agcttgcgca atacgacatc gcgatcttcg tctcggccaa tgcggtcgag
     5221 ttcggcgcac cgccgctcgg cgcgtggcca gcgacgatcg tcacttatgc gcctggcccc
     5281 ggaactgcgg aggcgctggc ggcggcagga atcgcgggcg cgttgcagcc gacgacttcg
     5341 ttcgacagcg aaggtctgct cgagctgccg acactcgccg atgtcaccgg aaaacgcgtc
     5401 gtcatctttc gtggcgaagg aggacgcgag tttctcggca gcacccttcg ctcgcgcggt
     5461 gcgacggttg accacgtgcc gtgctatcgc cgcgtcgcgc cgcagtcggg tgccgacggc
     5521 ctcgtcgaag cgatgcgcga aggccgcgcg catgcgctga cgctgacgtc gagcgaaggt
     5581 ctcgacaacc ttgtcgctgc cgtcggcgag gcgggacgct cgctgatggc gcagttgccg
     5641 gtgttcgccg cgcatccgcg catcgctgag cgcgcgcgcg agcacggcct gcgcgccgtc
     5701 gagacggcag gcggcgatgc cggactgctt gccggtgtgc tcgactggtt tgcggggccg
     5761 ccgcgcagcg ccgcgcggag cgcgtaagct cgcgggcaac aacgccagga tcgcattcca
     5821 cgcaaaggcg ccgctccatg aacgacatcg acatcctcct gacggcgtcg ctgccgccgt
     5881 ttctcgacca gccgttgcgc gcgga
//
LOCUS       DASSVX010000110         8915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_287400, whole genome shotgun sequence.
ACCESSION   DASSVX010000110 DASSVX010000000
VERSION     DASSVX010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8915
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287400"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFO53_04915"
     CDS             complement(<1..281)
                     /locus_tag="VFO53_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET8875650.1"
                     /translation="MRPGLDNQNEWTKIAGAAALGVVAMYMLDPDKGRRRRALVGDKA
                     YSVVSDARQALGAATRDAAHRIEGLRARARRLVSDTPIPDDLQLIERVRA"
     gene            complement(435..1343)
                     /locus_tag="VFO53_04920"
     CDS             complement(435..1343)
                     /locus_tag="VFO53_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET8875651.1"
                     /translation="MAGESTTAVIAAIVGNLAIAVCKFAAAAFSGSAAMLSEAIHSFV
                     DTGNDVLMLYGIRRSGKPADNDHPFGYGHELYFWTLVVGILIFALGGGMSILTGILHV
                     VNRTPAEASLWNYAVLAIAAVFEAISWYFGLRAFRSEQAGRGIIETIKVSKDPTTFSV
                     LLEDSAALVGLAFAFAGIYLSSHLDMPWLDGASSIAIGALLCAIALVMVYESKGLLVG
                     EGVGRATAQGLRTLIASDAAVQSIGKLATMYLGPDDVMLAVEIRFHDDNSLDDTRHAI
                     GRITRQIRDKYPRIRHVFLDASSIGN"
     gene            complement(1375..2562)
                     /locus_tag="VFO53_04925"
     CDS             complement(1375..2562)
                     /locus_tag="VFO53_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8875652.1"
                     /translation="MTDPGRPSPPWLALLLLWLAGNGLRLTILAVPPVIATIRDDLRL
                     SATEVGLLSSIPPAMFAIAALAGSLLVARLGVRVAMVGGLALVAAGSALRGASPNYVA
                     LLLTTIVMSAGVALMQPIMPTAVRQWVPARIALGTAVYTNGLLVGEILAVLLTIPVVL
                     PLVDGSWRLSLVAWSIPMAMIAVIVHAFAPRPAAPHAAGSTPMPRKWLPDWHVGLVWR
                     LGALFCCVNSIYFSANAFMPIYLASKGRPDLIGDALLALNLGQLPASLLLLGVAGRLE
                     RRAWPYVLSGVVSLASIAGIVLMVGPGTVAWSALLGFSDAAALILGLTLPPLLCRPED
                     VARTSAGTFMLSYGGAVVLALLSGVAWDVTQLPALAFVPLAVCALAMAVIAGAMWRKH
                     ELR"
     gene            2702..4885
                     /locus_tag="VFO53_04930"
     CDS             2702..4885
                     /locus_tag="VFO53_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8875653.1"
                     /translation="MTIRSHLLLLAIGVVLPVLAFAIFVSVVLVEQDRSTVERAAMDR
                     ARAMMTAVDAELRGSITTVLAIGASPALQTNDLAAFHAEARRVLATQPTWLDVTLTRP
                     SGEKLLDALKPLDRPGERVLDPASAAHVVRTGRLVIGNVDQLDASSPPGIPIRVPVML
                     AGHLAYVLTAVVRPESFQDIVRQQRLPDGWISGIVDANGNFVARIPARPAGEPASNAF
                     RAAVLRAPEGWYRGLTVEGRDAFTAHERSASSGWSIGLAIPAQIVEAAAWRTAWLMGI
                     GVALSIMIALGIAMLLGRRIAKPVVALASAARSIGSPGVPATIDDARVREVSDVAAAL
                     RDADIAVREREELIEREKSALQEADRAKDEFIAALSHELRNPLAALTAATHILRVADP
                     AHRAAADARGVIDRQTRQMSRMIEDLLDVSRLIAGKVHLRIETFDLAVLAATTVGAWR
                     ATGRFSGQTVTVDGRDGWVTADRARVEQILSNLLDNAVKFTPPGRTIAIRVARDANET
                     LLSVADGGEGIAPGLVDRVFDVFVQGDQDAGRSKGGIGLGLTIVKRLAQLQGGSVGAA
                     SAGTGHGATFTVRLPAAAPAVTVAAGAPASHGVSPRNILLVEDNDDARDMLRQVLAMQ
                     GHAVFEAAFGEDAIALAGQTRPDLAIVDIGLPDIDGYEVARRLKAQSPRRIALIALTG
                     YGQPDDVRRAQAAGFDVHLVKPISVERLDSAIAALCGALAAQSEA"
     gene            5025..6962
                     /gene="htpG"
                     /locus_tag="VFO53_04935"
     CDS             5025..6962
                     /gene="htpG"
                     /locus_tag="VFO53_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HET8875654.1"
                     /translation="MNETDAARPVRETMGFQAEVKELLGLMIHSLYSNREIFLRELIS
                     NASDACDKLRFEALHNPALLEGGSDLRIRVDYDAAARTISVADNGIGMNRDEVIANLG
                     TIAKSGTREFFRQLTGDEQKDAHLIGQFGVGFYSSFIVADKVTVFTRRAGELPDQGVR
                     WESDGGGEFAIEMAGKPERGTEITLHLREGQDDLSSGTKLRAIIHRYSDHIVQPIVMP
                     KEEWKDGKSQKVDETETVNQASALWARPKSAIGDDEYKAFYKHVGHDFDDPLAWVHAR
                     VEGRHDYTLLLYIPAHAPFDLWDRDARHGVKLYVRRVFIMDDAEQLLPAYLRFVRGVV
                     DSTDLPLNVSREILQESKDIETLRGGTTRKVLDLLAGLADGEKDKYATFWAQFGRVLK
                     EGIGEDYANKEKVAGLLRYASSAADTADETVSLADYVARMKEGQDSIYYVTAESFNAA
                     RNSPHLEVFRRKGIEVLLMSERVDEWVVGHLSAFDGKPLVSVAKGGLDLGKLEDEAEK
                     KEGEAEASELAPLIERMRTSLGERVKDVRVTRRLTDSPACLVADEHDVSANLARMLKA
                     AGQPAPASKPILEINPHHAVVLRLRDEERHFDDWAKVVFDQALLAEGGQLDDPAAFVR
                     RINELMLAMQTPASAAAQPST"
     gene            complement(6988..7404)
                     /locus_tag="VFO53_04940"
     CDS             complement(6988..7404)
                     /locus_tag="VFO53_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8875655.1"
                     /translation="MQQIKDVMSRDVKMVAPETTIREAAQQMRDGDFGMLPVAENDRM
                     VGAISDRDIAVRAVADDKGPETPVRDVMSRGICWAYEDDSVDVAARVMSERQVRRLPI
                     VNRDKRLVGIVSIGDFAVEPEDREPAAEALSGISQH"
     gene            7591..8685
                     /locus_tag="VFO53_04945"
     CDS             7591..8685
                     /locus_tag="VFO53_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HET8875656.1"
                     /translation="MDRRRFLVRGTIAAGGAAAVRQAVAQTPGLPSIRWRCASSFPKS
                     LDTIYGGAEVIAKRVEQLTGGKFQIRVFAAGEIVPAFGVVDAIQQGTIECAHTASYYF
                     VGKNKAFAFDTTLPFGLNQRQQNAWIYYGGGLELVRAFFKDFGIISFPAGNTGVQMGG
                     WWRKEVKTVNDLKGVKMRIAGLGGEVMARLGAVPQQIAGGDIYPALERGAIDSAEWVG
                     PYDDEKLGFYKVAPNYYFPGWWESNSMYSLYVNMKEWDKLPKEYQAAIEAAAYEANTD
                     MMAKYDFKNLAALQRLVKNGVKLHAFSNEIMLAAQKASYELYEEEASKNPAWKKIYEP
                     WKAFRGEEFLWHRAAEFTYESFAYSHPFGK"
BASE COUNT         1525 a         2971 c         3067 g         1352 t
ORIGIN      
        1 gcgcgcacgc gctcgatcag ttgcaggtcg tcggggatgg gcgtgtcgct gacgaggcgc
       61 cgcgcgcgcg cacgcagtcc ctcgatgcga tgcgccgcat cgcgcgtcgc cgcgccaagc
      121 gcctgccggg cgtcgctcac gacgctgtac gccttgtcgc cgaccagcgc gcggcgccgg
      181 cgtcccttgt ccggatcgag catgtacatc gcgaccacac cgagcgccgc cgcgcccgcg
      241 attttcgtcc attcgttctg attgtcgagt cctggtctca tgagcgtgtc ctcgctgtac
      301 tgccgccgga gcggatgcca accctgtcgc actcgcgcgc cgcaggtcaa aagcgcgacg
      361 ccgcgccttc gtctgcgcgt ttcggatcga cagcatatcc gaggcacaat tccacggctc
      421 cgtgtcagtt gccgtcagtt gccgattgac gacgcgtcca ggaacacgtg gcgaatccgt
      481 ggatatttgt cgcggatctg ccgtgtgatc cgcccgatcg catggcgcgt gtcgtcgagc
      541 gagttgtcgt cgtgaaaacg gatctcgacc gccagcatca cgtcatccgg gccaagatac
      601 atcgtcgcga gcttgccgat cgattgcacc gccgcatcgc tcgcgatcag cgtgcgcagt
      661 ccctgcgcgg tcgcgcggcc gacgccttcg ccgacgagca ggcccttgct ctcgtagacc
      721 atgacgagcg caattgcgca aagcagtgcg ccgatggcga tcgacgaggc gccgtcgagc
      781 catggcatgt cgagatggct gctgagatag atccccgcga aagcgaatgc gagtccgacg
      841 agcgccgccg aatcctcgag gagcaccgag aacgtggtcg ggtccttgct caccttgatc
      901 gtctcgatga tgccgcgacc cgcctgctcg ctgcggaaag cgcgcaggcc gaagtaccac
      961 gagatcgctt cgaacaccgc cgcgatcgcg agcaccgcgt aattccagag actcgcttcc
     1021 gccggcgtgc ggttgaccac gtgcaggatg ccggtcagga tcgacatgcc gccgccgagc
     1081 gcgaaaatca ggatgccgac gacgagcgtc cagaagtaga gctcatggcc gtaaccgaat
     1141 ggatgatcgt tgtcggctgg cttgccgctt cggcggatgc catacagcat caggacgtcg
     1201 ttgccggtat cgacgaatga atggatcgcc tcggacagca tcgccgcgct gcccgaaaat
     1261 gcggcggcgg cgaatttgca cacggcgatc gcgaggttgc cgacgatggc ggcgatcacg
     1321 gccgtggtcg attctcccgc cattcgcgtg tcgtcccttg tcgtcccttg ccgctcagcg
     1381 cagttcgtgc ttgcgccaca tggcgccggc gatgaccgcc atcgcgagcg cgcacaccgc
     1441 gagcggcacg aacgcgagtg ccggcagctg cgtgacgtcc cacgcgacgc cgctcagcaa
     1501 tgcgagcacg accgcaccgc cgtagctcag catgaacgtg ccggccgacg tgcgtgcgac
     1561 gtcctcgggc cggcacagga gcggcggcag cgtgaggccg aggatcagcg cggcggcgtc
     1621 ggagaagccc aggagcgccg accacgcgac ggtgccgggg ccgaccatca gcacgatgcc
     1681 cgcgatgctc gcgagcgaca ccacgccgga gaggacgtac ggccacgcgc ggcgctcgag
     1741 ccggcccgcg acgccgagaa gcagcagcga tgcaggcaac tggccgagat tcagcgcgag
     1801 cagcgcgtcg ccgatcagat ccgggcgacc cttgctggcg agatagatcg gcatgaacgc
     1861 attcgcgctg aagtagatgg agttgacgca gcagaacagt gcaccgagcc gccacacgag
     1921 gccgacgtgc cagtcgggca gccatttgcg cggcatcggc gtgcttcctg ccgcgtgcgg
     1981 cgcggcgggc cgaggcgcaa acgcgtgcac gatcaccgcg atcatcgcca tgggaatcga
     2041 ccatgcgacg agcgacaggc gccagcttcc gtcaacgagc ggcaggacga ccgggatcgt
     2101 gagcagcacg gcgagaatct cgccgacgag aagcccgttc gtatagaccg ccgtgccgag
     2161 cgcgatgcgc gcgggcaccc attgccgcac ggcggtcggc atgatcggct gcatcagcgc
     2221 aacgccggcc gacatcacga tggtggtcag cagcagcgca acgtagttcg gactggcgcc
     2281 gcgcagcgcc gatccggcgg cgaccagcgc aaggccgccg accatcgcga ctcgtacgcc
     2341 gaggcgggcg accagcagcg agcctgccag tgccgcgatc gcgaacatcg ccggcgggat
     2401 gctcgacaag agacccacct cggtcgcgct gagccgcagg tcgtcgcgta tggtcgcgat
     2461 caccggcggc accgcaagta tcgtgaggcg cagcccattg cccgccagcc agagcagcag
     2521 cagagcgagc caaggtggac ttgggcggcc ggggtcggtc atggggaacg tggacggccg
     2581 cgcgtagcgg acgtttgagc atcatgataa ctttgcgccc tcgtccggcg ccgagggcgc
     2641 caccggcgaa cgctcccgcg acccgtttcg catcgccatt ctcgcgccgt caccgaccgg
     2701 catgaccatc cgctcgcacc tgctgctgct cgcgatcggc gtcgtgctgc ccgtccttgc
     2761 gttcgccatc ttcgtttccg tcgtcctcgt cgagcaggac cgcagcacgg tcgagcgcgc
     2821 ggcgatggat cgggcacgcg caatgatgac cgccgtcgac gccgaactgc gcggatcgat
     2881 caccacggtg ctcgcgatcg gcgcgtcgcc cgcgttgcag acgaacgacc tcgccgcgtt
     2941 ccacgccgag gccaggcgcg tgctggcaac gcagccgacc tggctcgatg tgacgctgac
     3001 gcgaccctcg ggcgagaagc tgctcgatgc gctgaagcca ctcgaccggc cgggcgagcg
     3061 cgtcctcgat ccggcgagtg ctgcgcacgt cgtgcggacc ggccggctcg tcatcggcaa
     3121 cgtcgaccag ctagacgcct cgtcgccgcc gggcattccg atacgtgttc cggtgatgct
     3181 ggccgggcac ctcgcctacg tgctgaccgc ggtcgtgcgt cccgaatcct ttcaggacat
     3241 cgttcgccag cagcgtctgc cggacggctg gatcagcggc atcgtcgacg ccaacggaaa
     3301 tttcgtcgcg cgcattcccg cccggcccgc gggcgagccg gcgtcgaatg cgttccgcgc
     3361 ggcggtgctg cgcgcacccg aaggctggta ccgcggactg acggtcgagg gcagggacgc
     3421 gttcaccgcg cacgagcgct ccgcgtccag cggatggagc atcggcctcg ccattcccgc
     3481 gcaaatcgtc gaggccgcgg cctggcggac ggcctggctg atgggtatcg gcgtggcgct
     3541 gtcgatcatg atcgcgctcg gcatcgcgat gctgctcggc cgccggatcg ccaagccggt
     3601 cgtcgcgctg gcgtccgcgg cacgttcgat cgggtcgcct ggcgtgcctg cgaccatcga
     3661 cgacgcccgc gtgcgcgagg tgagcgacgt ggccgcggcg ctgcgcgacg ccgacattgc
     3721 ggtgcgcgag cgcgaggagc tgatcgagcg cgagaaaagc gcgctgcagg aggccgaccg
     3781 cgccaaggac gaattcatcg ccgcgctgtc gcacgagctg cgcaatccgc tggcggcgct
     3841 gacggcggcg acgcatatcc ttcgcgtggc cgatcccgcg caccgcgcgg cggcggatgc
     3901 gcgcggcgtc atcgatcgcc agacgcgaca gatgtcgcgc atgatcgagg acctgctcga
     3961 cgtgagccgc ctcattgcgg gcaaggtgca cttgcgcatc gagacgttcg acctcgcggt
     4021 actcgcagca acgaccgtcg gcgcctggcg cgccacggga cgcttcagcg ggcagaccgt
     4081 caccgtcgac ggccgggacg gctgggtgac ggccgatcgg gcgcgcgtcg agcagatcct
     4141 gtcgaacctg ctcgacaacg cggtcaagtt cacgcccccc ggcaggacga tcgcgattcg
     4201 cgtcgcccgg gacgccaacg agacgctgtt gtcggttgcg gatggtggcg aagggattgc
     4261 gcccgggctc gtcgaccgcg tcttcgacgt tttcgtgcag ggcgaccagg atgcggggcg
     4321 ctcgaagggc ggcatcgggc tcggcctcac gatcgtgaag cgtctcgccc aattgcaggg
     4381 tggctcggtc ggcgccgcga gcgccggcac tggccacggt gcgacgttca cggtgcgcct
     4441 tcccgccgcc gcgccggccg tcaccgtcgc ggccggcgcg ccggcgtcgc atggcgtgtc
     4501 gccgcggaac atcctgctcg tcgaggacaa cgacgatgcg cgcgacatgc tccgccaggt
     4561 gctcgcgatg cagggtcatg cggtattcga agcggcgttc ggcgaagacg cgatcgcgct
     4621 cgccgggcag acacggccgg acctggccat cgtcgacatc gggctgcccg acatcgacgg
     4681 ctacgaggtt gcgcgacggc tcaaggccca atcgccacga cggatcgcgc tgatcgcact
     4741 gaccggttac gggcagcccg acgacgtgcg gcgcgcgcag gccgcaggct tcgacgtgca
     4801 cctcgtgaag ccgatcagcg tcgagcggct cgacagtgcg atcgcggcgc tgtgcggagc
     4861 gctggcggcg cagtccgagg cttgacgcgg cgggccgact tgcgacccgc gccgacacgc
     4921 gcggggcagc ggccgacgca cccgccaggc aagcgcgcgc atggccagcg cggcccgagc
     4981 gattacgata tgcgtctcac cgtgacaaac ccgcgatccc agccatgaac gaaacagacg
     5041 ctgcccgccc cgtgcgcgaa acgatgggct tccaggccga ggtgaaggaa ctgctcggcc
     5101 tcatgatcca ttcgctttat tcgaaccgcg agatcttcct gcgcgagctg atctccaacg
     5161 cgtcggatgc ctgcgacaag ctgcgcttcg aggcgctgca caatccggcg ctgctcgaag
     5221 gcggcagcga cttgcgcatc cgggtcgact acgacgcggc ggcgcgcacg atcagcgtcg
     5281 ccgacaacgg catcggcatg aatcgcgatg aagtcatcgc caacctcggc acgatcgcca
     5341 agtcgggcac gcgcgagttt ttccgccagc tcaccggcga cgagcagaag gacgcgcatc
     5401 tgatcggcca gttcggcgtg ggcttctact cgtcgttcat cgtcgccgac aaggtgacgg
     5461 tgttcacgcg ccgcgccggc gagcttcccg accagggcgt gcgctgggag tcggatggcg
     5521 ggggcgaatt cgcgatcgag atggccggca agccggagcg cggcaccgag atcacgctgc
     5581 acctgcgcga aggccaggac gacctttcgt ccggaacgaa gcttcgcgcg atcatccacc
     5641 gctactccga tcacatcgtg cagccgatcg tgatgccgaa ggaggagtgg aaggacggca
     5701 aatcgcagaa ggtcgacgaa accgaaaccg tgaaccaggc gtcggcgctg tgggcacggc
     5761 cgaaaagcgc gatcggcgac gacgagtaca aggcgttcta caagcacgtg ggtcacgact
     5821 tcgacgaccc cctcgcctgg gtgcatgccc gcgtcgaggg ccgccacgac tacacgctgc
     5881 tgctctacat cccggcgcac gcgccgttcg acctgtggga ccgcgacgcc cggcatggcg
     5941 tcaagctcta cgtgcgccgc gtcttcatca tggacgacgc cgagcagctg ctgccggcgt
     6001 acctgcgctt cgtgcgcggc gtcgtcgatt cgaccgacct gccgctcaac gtgtcgcgcg
     6061 agatcctgca ggaatcgaag gatatcgaga cgctgcgcgg cggcacgacg cggaaggtgc
     6121 tcgatctgct tgcgggcctt gccgacggcg agaaggacaa gtacgcgacg ttctgggcgc
     6181 aattcgggcg cgtgctgaag gaaggcatcg gcgaggacta cgccaacaag gagaaggtgg
     6241 cggggctgtt gcgttacgcg tcgagtgcgg ccgacaccgc ggacgagacc gtgtcgctcg
     6301 ccgattacgt cgcacggatg aaggagggcc aggacagcat ctattacgtc accgccgaat
     6361 cgttcaacgc cgcccggaac agtccgcatc tcgaggtgtt ccggcgcaag ggcatcgagg
     6421 tgctgctgat gtcggagcgc gtcgacgagt gggtggtcgg tcacctcagc gcgttcgacg
     6481 gcaagccact cgtgtcggtc gcgaagggcg ggctcgatct cggcaagctc gaggacgagg
     6541 ccgagaagaa ggaaggcgag gccgaggcga gcgagctcgc gccgctcatc gagcggatgc
     6601 gaacgagcct cggtgagcgc gtgaaggacg tgcgcgtgac gcgacggctg accgactcac
     6661 ccgcctgcct cgtcgccgac gagcacgacg tgagcgcgaa cctcgcgcgc atgctgaagg
     6721 cggcgggcca gccggcgccg gcgtcgaagc cgattctcga gatcaatccg caccacgcgg
     6781 tggtgctgcg cctcagggac gaggagcgtc atttcgacga ctgggcgaag gtggtgttcg
     6841 accaggcgct gctcgccgaa ggcggccagc tcgacgatcc cgcggcgttc gtcaggcgta
     6901 tcaacgagct gatgctggcg atgcagacgc cggcgtcggc tgccgcgcag ccgtccacgt
     6961 gagcacgctc gccgtgcgcg agcgcggcta gtgctgcgaa attcccgata gcgcctcggc
     7021 cgccggctcg cggtcctcgg gctcgacggc gaaatcgccg atcgacacga tgccgacgag
     7081 gcgcttgtcg cggttgacga tcggcagccg gcgcacctgc cgctcgctca tcacgcgcgc
     7141 cgcgacatcc accgaatcgt cctcgtaggc ccagcagatg ccgcgcgaca tcacgtcgcg
     7201 caccggcgtt tccggaccct tgtcgtcggc aacggcgcgc acggcgatgt cgcgatcgga
     7261 gatcgccccg accatgcgat cgttctcggc gacaggaagc atgccgaaat cgccgtcgcg
     7321 catctgctgc gcggcttcgc ggatcgtcgt ttccggcgcg accatcttca cgtcgcggct
     7381 catcacgtcc ttgatttgtt gcatggttac ctccgcagtc tccggtgccc ggtgtcgcgc
     7441 gtccgcgcgc gggccgaacc acgttcgaca cgccgcttcg agccgcgttc tcgtggtcat
     7501 ccggcggcgt tttgggtaga ctcgcctcgc ctcgccccgc cccgccccgg tgtggggacg
     7561 gccgggcacg acaaacatca ggaggagacg atggatcgac gcaggtttct cgtacgcggg
     7621 acgatcgccg ccggtggtgc ggccgcggtc aggcaggcgg ttgcgcaaac gccgggcctg
     7681 ccctcgatac gctggcggtg cgcatcgagc tttccgaaga gcctcgacac gatctacggc
     7741 ggcgccgagg tcattgccaa gcgcgtcgag cagttgaccg gaggcaagtt ccagattcgc
     7801 gtgttcgccg ccggcgaaat cgtgccggcg ttcggcgtcg tcgatgcgat ccagcaggga
     7861 acgatcgagt gcgcgcacac ggcgagctac tacttcgtcg gcaagaacaa ggcgttcgcg
     7921 ttcgacacga cgcttccgtt cgggctcaac cagcgccaac agaacgcctg gatctattac
     7981 ggcggcgggc tcgagctcgt gcgcgcgttc ttcaaggact tcggcatcat ttcgttcccg
     8041 gcgggcaaca ccggcgtgca gatgggcggc tggtggcgca aggaagtgaa gacggtgaac
     8101 gacctcaagg gcgtgaagat gcgcatcgcc gggctcggcg gcgaagtgat ggcgcgcctt
     8161 ggcgcggtgc cgcagcagat cgccggcggc gacatctatc cggcgctcga gcgcggcgcc
     8221 atcgactcgg ccgaatgggt gggcccgtac gacgacgaga agctcggctt ctacaaggtg
     8281 gcgccgaact attactttcc cggatggtgg gagagcaact cgatgtactc gctctacgtg
     8341 aacatgaagg aatgggacaa gctgccgaag gagtaccagg cggcgatcga ggcggccgcg
     8401 tacgaggcga acaccgacat gatggccaag tacgatttca agaacctggc cgcgctgcaa
     8461 cggctcgtca agaacggcgt caagctgcac gccttctcca acgagatcat gctcgcggca
     8521 cagaaggcgt cgtacgagct gtacgaggaa gaggcgtcga agaatccggc gtggaagaag
     8581 atctacgagc cgtggaaggc gtttcgcggc gaggagttcc tgtggcaccg cgccgcggag
     8641 ttcacctacg agagcttcgc gtacagccac ccgttcggga aataaccgcg tcccgacaaa
     8701 ggcaggggat ccagcgaggt tcgtcgtccc cgcgaaggcg gggacccagc ggcttattcg
     8761 agcgaaagac gcgtacccag cgagcttcgt cgtccccgcg aaggcgggga cccagcggct
     8821 tattcgagcg aaagacgctg ggttcccgct tccgcgggaa cgacgcggct ttgcgaatgg
     8881 tcgcgtagtt cgtaatactc gataggcggt ttact
//
LOCUS       DASSVX010000111         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_720642, whole genome shotgun sequence.
ACCESSION   DASSVX010000111 DASSVX010000000
VERSION     DASSVX010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720642"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..529)
                     /locus_tag="VFO53_04950"
     CDS             complement(98..529)
                     /locus_tag="VFO53_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET8875657.1"
                     /translation="MPTARTPPIYTSPQDVTQAFYQAFETRDIDAMMATWAEDEDIVC
                     IHPGGMRHVGYESVRHSFDQLFAGGTKLTFRLDQVVVLETVGLAMQSAIEHLQAGDEG
                     ARGAAVATNVLMRTPSGWRIVCHHASPAPAVTEAPASGPLH"
     gene            complement(541..1572)
                     /gene="waaF"
                     /locus_tag="VFO53_04955"
     CDS             complement(541..1572)
                     /gene="waaF"
                     /locus_tag="VFO53_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HET8875658.1"
                     /translation="MLVIAPSWVGDAILSEPLIASLRNVAGTSLEIDVVAPPWCGPVY
                     ARMRGVARIIDAPAAHGEFALRQRRRLGIALRERGYSRAYVLPNTWKSALVPWFARIP
                     TRIGYVGEARHGLLTQPRRLDTRAMPQLVTRYAALASPAGTSPREPPVPVLVPNAANL
                     AAAMSAMALDRSRKVAILCPGAEYGPAKRWPVEYFADLARRLSADGHAIWILGSPNDA
                     PVAAPIIAALGETALPPRDLTGRTDLGTAIDLMSVASIVVSNDSGLMHAAAAVRAPLV
                     ALFGSSSPAYTPPLSDTARIARIDIVCSPCFERECPLGHFRCMRDLAPELVYNLARAT
                     SDAAPPPPH"
     gene            complement(1632..1820)
                     /locus_tag="VFO53_04960"
     CDS             complement(1632..1820)
                     /locus_tag="VFO53_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HET8875659.1"
                     /translation="MTAAQGPVVVHANDLPIYCPNPAMPLWSSHPRVFLDVAGAGEAM
                     CPYCGTRYRLEGDAKAGH"
     gene            complement(1817..>2617)
                     /locus_tag="VFO53_04965"
     CDS             complement(1817..>2617)
                     /locus_tag="VFO53_04965"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET8875660.1"
                     /translation="RCYKTDRGPAIFRLRDHTDRLFRSARIFGMKIPFTAEVLIEAQR
                     ACVRDNKLDSCYLRPIAFYGSVAMGVAAKSNPVLVAIAAWPWGAYLGADGLEKGIRVK
                     TSSYTHHHPNITMCGAKAVANYTVSILANQEATHDGYEEAMLLDPQGFVCQGSGENVF
                     LVRDGKLHTPDLSGGALAGITRETIIQFAGELGIPVIERRITRDEFYVADEAFFTGTA
                     AEVTPIREYDHRPIGDGRRGPITTKLQTMFFDAVYGRDAAHGEWLTLV"
BASE COUNT          453 a          854 c          880 g          430 t
ORIGIN      
        1 atggcctcac cgtcggcggc acctcctgaa accgatttca gaacaggctg cgcgacccga
       61 tagctttggc gtgagcgaac gccgccgata ggctctctta atggagaggt cctgacgcgg
      121 gcgcttcggt aacagcgggc gccggcgacg cgtgatgaca cacgattcgc cagcctgacg
      181 gcgtgcgcat caacacgttg gtcgcgaccg ctgcgccgcg cgcaccttcg tcgcccgcct
      241 gcagatgctc gattgcactc tgcatcgcga gccccaccgt ttcgagcacg acgacctgat
      301 cgaggcggaa cgtgagctta gtgccgccgg cgaacagctg atcgaagctg tgccggaccg
      361 attcgtagcc gacgtgccgc atgccgcccg gatgaatgca gacgatgtcc tcgtcctcgg
      421 cccacgtcgc catcatcgca tcgatgtcgc gcgtctcgaa cgcctggtag aacgcctgcg
      481 tgacgtcctg cggcgacgtg tagattggag gcgttcgtgc cgtcggcatg gcggtgatga
      541 tcagtggggc ggcggtggcg ccgcatcgga tgtcgcgcgc gccagattgt agaccaactc
      601 cggcgcaaga tcgcgcatgc agcgaaaatg tccgagcgga cattcacgct cgaagcaggg
      661 gctgcagacg atgtcgatgc gcgcgatccg cgccgtatca gacaacggtg gcgtataagc
      721 cggcgacgac gagccgaaca gcgcaacgag cggcgcgcgc acggccgctg ccgcgtgcat
      781 cagccccgag tcgttgctga cgacgatcga tgcgacggac atcaagtcga tcgccgtgcc
      841 gaggtcggtg cggccggtga gatcgcgagg cggcagtgcg gtttcgccaa gtgctgcgat
      901 gatcggcgcc gccaccggcg catcgttggg cgagccgaga atccagatcg catgaccatc
      961 cgcgctcaag cgccgcgcaa gatcggcgaa gtattcgacg ggccagcgct tggccggccc
     1021 gtactcggcg cccggacaca ggatcgcgac cttccgcgat cgatcgagtg ccatcgcact
     1081 catcgcagcg gcgaggttgg ccgcattggg aacgaggacc ggcaccggcg gctcgcgagg
     1141 cgatgtgccg gcggggctcg cgagcgccgc gtagcgggtg acgagctgcg gcatcgcgcg
     1201 cgtgtcgagt ctgcgcggct gtgtcaacaa gccgtgccgc gcttcaccca cgtagccgat
     1261 tcgcgtggga attcgcgcga accatggaac cagcgcagat ttccacgtat tcggcagcac
     1321 gtacgcgcgg gagtagcctc gctcacgcag cgcgatgccg agtcgacgac gctgccgcag
     1381 cgcgaactcg ccgtgcgcag cgggcgcatc gatgatccgc gcgacaccgc gcatgcgcgc
     1441 atagaccggc ccgcaccacg gcggcgcgac gacgtcgatt tcgagcgacg tacctgcgac
     1501 attccgcagg ctggcgatca gcggttcgga caggatcgcg tcgcccaccc acgacggcgc
     1561 gataacgagc acgcgttcgc cgctgccgcg cgcggacgcg cgcgatgcgg cttcgacgtc
     1621 gtttgcggcg ctcagtgtcc cgctttcgcg tcgccttcga ggcggtaacg cgttccgcaa
     1681 tacgggcaca tggcttcgcc ggcgcccgcg acgtcgagaa agacgcgcgg atgcgagctc
     1741 cacaacggca tcgcagggtt cgggcagtag atcggcagat cgttcgcgtg cacgaccacc
     1801 ggcccttgcg ccgccgtcat acgagcgtca gccattcgcc atgcgcggcg tcgcgtccgt
     1861 agacagcgtc gaagaacatc gtctgcagct tcgtcgtgat cggcccgcgg cggccatcgc
     1921 cgatcggccg gtgatcatat tcgcgaatcg gggtcacctc ggcagcggtg ccggtgaaaa
     1981 acgcttcgtc ggcgacgtag aactcgtcgc gtgtgatccg ccgttcaatg acgggaatgc
     2041 cgagctcgcc tgcgaactgg atgatcgttt cccgcgtgat gcccgcgagc gcgccgccgg
     2101 aaagatcggg cgtgtgcagt ttgccgtcgc ggacgaggaa cacattctcg ccggagccct
     2161 ggcagacgaa accctgcggg tcgagcagca tcgcttcctc gtacccgtcg tgcgtcgctt
     2221 cctggttggc gaggatggac accgtgtaat tcgcgactgc cttcgccccg cacatcgtga
     2281 tgttcgggtg gtggtgcgtg tacgacgacg tcttcacgcg gatgcctttc tcgaggccgt
     2341 cggcacccag gtacgcgccc caaggccacg cggcgatcgc gaccagcacc gggttcgatt
     2401 ttgccgcgac gcccatcgcc accgagccgt aaaaagcgat cggccgcagg tagcacgagt
     2461 cgagcttgtt gtcgcgaacg cacgcgcgct gtgcctcgat caggacttca gcggtaaagg
     2521 gtattttcat gccgaagatg cgtgcggaac ggaacaatcg gtccgtgtga tcgcgcaatc
     2581 ggaagattgc cgggccgcga tccgtcttgt agcagcg
//
LOCUS       DASSVX010000112         6041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1470570, whole genome shotgun sequence.
ACCESSION   DASSVX010000112 DASSVX010000000
VERSION     DASSVX010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470570"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /gene="rho"
                     /locus_tag="VFO53_04970"
     CDS             <1..546
                     /gene="rho"
                     /locus_tag="VFO53_04970"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET8875661.1"
                     /translation="HVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVPASG
                     KVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIYEEFKGTGN
                     MEIHLDRRMAEKRIYPAINVNRSGTRREELLLKPEILQKVWVLRKLLYPMDELEAMEF
                     LLDKVKATKSNAEFFESMRRG"
     gene            663..857
                     /locus_tag="VFO53_04975"
     CDS             663..857
                     /locus_tag="VFO53_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HET8875662.1"
                     /translation="MARKYIDCRLFPSDTKCSIAISGDTEDEVVAAAAQHAVSVHKHE
                     DSPQLRQQIRASVKEGSPAS"
     gene            1063..1284
                     /gene="rpmE"
                     /locus_tag="VFO53_04980"
     CDS             1063..1284
                     /gene="rpmE"
                     /locus_tag="VFO53_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET8875663.1"
                     /translation="MKIDTHPKYTEINVTCSCGNTFTTRSTISKPLHVEVCSACHPFY
                     TGKQKIMDTAGRVEKFRQRYGTKTATPSA"
     gene            1372..3075
                     /locus_tag="VFO53_04985"
     CDS             1372..3075
                     /locus_tag="VFO53_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875664.1"
                     /translation="MRSLGRPALDTAPSAAPDAADPYRAWKQAGLLLLAVAWIALGLT
                     GHDPWKFDDATTFGVAWEMAQRHDYVVPHLAGEVYLRNPPLVPAIAAATQQLLSPPLE
                     PFDAARVAAGGALALILLFTALASGELAGPTYRWPPVLILIGSVGLWDRAHVLSGELG
                     VMVGLAIALYGQALALRRPVVGGAWTGAGAAVAFLCQGFAGPAWIAIAALLLVPLGPQ
                     FRSRRYVAALGIALVVAVALAAPWIVALYLRDSSLLYEWLATEALGQHVAFFGDPSLA
                     DPLYHARNLLWFAWPALPLIAWMVWTRARGFNGGLAEPRVLIPAILALVIYANLLVVP
                     EPRLIQALPLLVPCALLASLEIDSLARSFSAALDWFGILTFGLLAIVVWGIWVDARLN
                     GMSPAIAALFRDTEVGFQPTFHLGSVIAAMVLTLLWIALVRPARRSNRRALLNWAAGV
                     TLAWCLYSTIWLPYLDSRRSYRTMIENLDAHLPVAGCIASRNLGDPQRALLYYFAAIE
                     TVREEAQPNADDECPALLVQYGAIGGAAPALSGWHVEWEGGRRGDATERYVLYMKDDA
                     S"
     gene            3072..4193
                     /gene="obgE"
                     /locus_tag="VFO53_04990"
     CDS             3072..4193
                     /gene="obgE"
                     /locus_tag="VFO53_04990"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET8875665.1"
                     /translation="MKFVDEAYIEVIAGDGGNGAASFRREKYVPRGGPDGGDGGRGGS
                     IHAVADRNINTLVDYRFARIFRAKRGENGRGADKYGHGADDIVLRVPVGTVISDAGSG
                     DVIADLAQDGARALVAKGGKGGLGNLHFKSSTNRAPRQTTPGEKGEERRLHLELKVLA
                     DVGLLGLPNAGKSTLIRAISAARPKVADYPFTTLAPQLGVVRTSEAHSFVVADIPGLI
                     EGAAEGAGLGHQFLRHLARTRLLLHVVDMAPLDPDADPLAGARAIVRELGKYDAGLAA
                     KPRWLVLNKIDLIAEEERAARCAAFAKSLRYKGPLFAISAIDGTGCRELTFAIQSWLD
                     EHPPVTAETGTAPADAGAPVVLTPAPVAPRRRRRTDEAP"
     gene            4190..5308
                     /gene="proB"
                     /locus_tag="VFO53_04995"
     CDS             4190..5308
                     /gene="proB"
                     /locus_tag="VFO53_04995"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET8875666.1"
                     /translation="MTPFADASRIVVKVGSSLVTNEGRGIDHDAVARWAGEIARLAEA
                     GKAIVVVSSGAIAEGMQRLGWSTRPRAIHDLQAAAAVGQMGLVQAYERAFARHSLRTA
                     QVLLTHEDLSDRRRYLNARTTLTTLIALRVIPIINENDTVTTDEIRFGDNDTLGALVT
                     NLIEADLLVLLTDQQGLFSADPRKHPAATLVRSARAGDPALEAMAGGAGSDIGRGGML
                     TKVLAAKRAARSGASTIIACGREADVLTRLARGEPLGTTLAAERSSLAARKQWLADHV
                     RLAGRLTLDSGAVHALTRDGKSLLPIGVVAVTGNFGRGEVVGCIAPDGREVARGLVNY
                     GAQEAARIVRRPSSEIESVLGYVDEPELIHRDNLVLLA"
     gene            5305..5868
                     /locus_tag="VFO53_05000"
     CDS             5305..5868
                     /locus_tag="VFO53_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HET8875667.1"
                     /translation="MSGTGVVERARRILADPRDEWIKIAAEPATVQSLYTNWIMILAA
                     IGPIALLLSMHPLQVAVAQYVRSLITTFVLALVVDALAPSFGGSKDFIASLKLTAYSY
                     TAAWLAGVFNMLGALGDLVILIATLYALYTFLVGAPVLNRSSPDKAVPFTLVVVLCAV
                     VLFFLARFAFTGMLAPPMHAGMGLAIR"
BASE COUNT          937 a         2102 c         2007 g          995 t
ORIGIN      
        1 cacgtgcagg tcgccgagat ggtgatcgag aaggcgaagc gcctcgtcga gcacaagaag
       61 gacgtcgtga tcctgctcga ttcgatcacc cgccttgcgc gcgcctacaa cacggtcgtg
      121 ccggcgtcgg gcaaggtgct caccggtggc gtggacgcca atgcgctgca gcgcccgaag
      181 cgcttttttg gcgcggcgcg caacatcgag gaaggcggct cgctgacgat catcgccacc
      241 gcgctgatcg acaccggctc gcggatggat gacgtgatct acgaggagtt caagggcacc
      301 ggcaacatgg aaatccatct cgaccggcgc atggccgaga agcgcatcta cccggccatc
      361 aacgtgaacc gctcggggac gcggcgcgag gagttgctgc tgaagccgga gatcctgcag
      421 aaggtctggg tcctgcgcaa attgctctac ccgatggacg agctcgaggc gatggaattc
      481 ctgctcgaca aggtcaaggc aaccaagagc aacgccgagt tcttcgaaag catgcggcga
      541 ggctgatccg tttcgcagct gttgcgcccg cgattgcgtt ggcgcaccat cgcgacatcg
      601 cacgatcccg accagcccga cggcgggacc cgcgcatcgc ccgcccacca aggagagcgt
      661 ccatggcacg caaatacatc gactgccggc tgttccccag cgacaccaaa tgttcgatcg
      721 cgatctccgg cgataccgag gacgaggtcg tcgccgcggc ggcgcaacac gcggtcagcg
      781 tgcacaagca cgaggattcg ccgcagcttc gccagcagat ccgcgcgagc gtgaaggaag
      841 gcagcccggc gtcctgaaaa cccggcggcc ggccgcgcgc cggttttttg tcccttccaa
      901 tcaatgggtt gcggcgccgc ttgcaaagcg tcgccgacac cgcaacttgc gataaaccgc
      961 catctcgcgg ataatcgctg attcccttgg ctctcgccac ccgctcgctt cgcgggcccg
     1021 tgcgtcgagc cgcgcgccag gcaaacggaa ggcacccgga ccatgaaaat cgacacccat
     1081 cccaaataca ccgaaatcaa cgtcacgtgc agctgcggca acacgttcac cacgcgctcg
     1141 acgatcagca agccgctgca cgtcgaagtc tgctcggcat gccatccgtt ctacaccggc
     1201 aagcagaaga tcatggacac ggcaggtcgc gtcgagaaat tccgccagcg ctatggcacc
     1261 aagacggcga cgccatcggc ctgacgccat tgtcgttccg cgtcgcccaa agaggcagct
     1321 tcggctgcct ttttcgttgc agtcgcgaag cgtagccgcc attcttccgc catgcgcagc
     1381 ctcggccgac ccgccctcga cacagcaccg tccgcagcgc ccgatgccgc ggacccgtat
     1441 cgggcgtgga agcaggcggg cctgcttctg ctggccgtcg cctggatcgc gctcgggctg
     1501 acgggccacg atccgtggaa gttcgacgac gccaccacgt tcggcgtcgc atgggaaatg
     1561 gcgcagcgtc acgattacgt cgtgccgcat ctcgcgggcg aagtctacct gcgcaatccg
     1621 cccctcgtgc cggcgatcgc cgcggcgacg cagcaactgc tttcaccgcc gctcgagccg
     1681 ttcgacgccg cgcgcgttgc cgcgggcggc gcactggcgc tgattcttct gttcacggca
     1741 cttgcgagcg gcgagctcgc cggcccgacc taccgctggc cgccggtgct gatcctgatc
     1801 ggctcggtgg gtctttggga tcgcgcgcac gtgctgtcgg gcgagctcgg cgtcatggtc
     1861 ggtctcgcca tcgcgctcta tggacaggcg cttgcgctgc gccgcccggt tgtcggcggg
     1921 gcgtggacgg gtgccggcgc agccgtcgcg ttcctctgcc agggattcgc gggtccggcg
     1981 tggattgcga tcgcagcgct gctcctcgtg cccctcggcc cgcagtttcg cagccgccgt
     2041 tacgtcgcgg cgctcggcat cgcgctcgtc gttgccgtgg cgcttgcggc accgtggatc
     2101 gtcgcgctgt acctgcgcga ttcgtcgctg ctctacgagt ggctcgcgac cgaagcgctc
     2161 ggacagcatg tcgcgttctt cggcgatccg tcgctggccg atccgctgta tcacgcgcgc
     2221 aacctgctgt ggttcgcatg gcctgcgctg ccgctgatcg cctggatggt ctggacgcgc
     2281 gcccgcggct tcaacggcgg ccttgccgag ccgcgggtgc tgatcccggc gattctcgcg
     2341 ctcgtcatct acgcgaatct gctcgtggtt cccgagccga ggctgatcca ggcgctgccg
     2401 ctgctcgtgc cctgcgcgtt gctcgcgtcg ctcgagatcg attcgctggc gcgcagcttt
     2461 tcggcggcgc tcgactggtt cggcatcctg acgttcgggt tgctcgcgat cgtcgtctgg
     2521 ggcatctggg tcgatgcacg gctgaacggc atgtcgccgg ccatcgcggc gcttttccgc
     2581 gacaccgaag tcggcttcca gccaaccttc cacctggggt cggtgatcgc ggccatggtt
     2641 ctgacgctgt tgtggatcgc gctcgtgcgg ccggcgcggc gcagcaatcg ccgcgcgctc
     2701 ctcaactggg cggcgggggt cacgcttgcc tggtgcctct actcgacgat ctggctgccg
     2761 tatctcgatt cgcggcgctc gtaccgcacg atgatcgaga atctcgacgc gcatctgccc
     2821 gttgccggtt gcatcgcgag ccgcaatctc ggcgacccgc agcgcgcgtt gctgtactac
     2881 ttcgcggcga tcgaaacggt gcgcgaggaa gcgcagccca atgccgacga cgagtgcccc
     2941 gcacttctcg tgcaatatgg tgccatcggc ggtgccgcgc ccgcgctgag cggctggcac
     3001 gtcgaatggg aaggcggccg gcgcggcgac gccaccgagc gctacgtgct ttacatgaag
     3061 gatgatgcgt cgtgaaattc gtcgatgaag cgtacatcga ggtcatcgcc ggcgacggcg
     3121 gcaacggcgc cgcctcgttc cggcgcgaga agtacgtgcc gcgcggcggg cccgacggcg
     3181 gcgacggcgg ccggggcggc agcatccacg cggttgccga ccgcaacatc aacacgctcg
     3241 tcgactatcg cttcgcgcgc atcttccgcg cgaagcgcgg cgagaacggg cgcggcgcgg
     3301 acaagtacgg gcatggcgcc gacgacatcg tgctgcgggt gccggtgggc acggtgatca
     3361 gcgatgcggg cagcggcgat gtcattgccg atcttgcgca ggatggcgcc cgtgcgctcg
     3421 ttgcgaaggg cggcaagggc gggctcggca accttcattt caagtcgagc accaatcgcg
     3481 ccccgcgcca gacgacgccc ggcgaaaagg gcgaggagcg gcggctgcat ctcgagctca
     3541 aggtgctggc cgacgtcggc cttctcggct tgccgaatgc gggcaagtcg acgctgatcc
     3601 gcgccatttc cgcggcgcgt cccaaggtcg ccgactatcc attcacgacg ctcgcgcccc
     3661 aactcggcgt cgtgcgaacc tcggaagcgc atagctttgt cgtcgccgac atccccggat
     3721 tgatcgaagg cgccgcggaa ggcgcaggcc ttggccacca gttcctgcgc catctcgcgc
     3781 gcacgcgcct gctgctgcac gtcgtcgaca tggcaccgct cgacccggac gccgatccgc
     3841 tcgccggtgc gcgagcgatc gtcagggaac tcggaaaata cgacgccggg ctcgctgcca
     3901 agccgcgctg gctcgtgctg aacaagatcg acctcatcgc ggaagaggag cgcgccgcgc
     3961 ggtgcgcggc gttcgcgaag tcgctgcgct acaaggggcc gcttttcgcg atctcggcga
     4021 tcgacggcac cgggtgccgc gagttgacgt tcgccatcca gtcctggctc gacgagcatc
     4081 caccggtgac ggccgagacc ggcacggcgc ccgccgacgc gggcgcaccg gtcgtgctga
     4141 cgccggcacc cgtcgcaccg cgccggcgcc gcaggaccga cgaggcacca tgacgccgtt
     4201 cgcggatgcg tcgcggatcg tcgtcaaggt cggctcgagc ctcgtgacca acgagggacg
     4261 cggcatcgat catgacgcgg tcgcgcgctg ggccggcgaa atcgcacggc tcgccgaagc
     4321 gggaaaagcc attgtggtcg tttcgtccgg ggccattgcc gaggggatgc agcggctcgg
     4381 ctggtcgacc cgcccacgcg ccatccacga tctccaggcg gcggccgcgg tcgggcagat
     4441 gggtctcgtg caggcgtacg agcgcgcgtt cgcgcgccac tcgttgcgta cggcgcaggt
     4501 tctgctcacg cacgaggacc tttccgatcg gcggcgctat ctcaatgcgc gcacgacgct
     4561 caccacgctc atcgcgttgc gcgtgattcc gatcatcaac gagaacgaca ccgtcaccac
     4621 cgacgagata cgcttcggcg acaacgacac gctcggcgcg cttgtcacca acctgatcga
     4681 agccgatctt ctggtgctac tcaccgacca gcagggactg ttcagcgccg atccgcgcaa
     4741 gcatccggcc gccacgctcg tgcgcagcgc gcgcgccggc gatccggcac tcgaagcgat
     4801 ggcgggcggc gcgggcagcg acatcggacg cggcggcatg ctgacgaagg tgctggcggc
     4861 caaacgcgcg gcgcgctcgg gcgcatcgac gatcatcgcg tgcggacgcg aagccgacgt
     4921 gctgacgcga ctcgcccgcg gagagccgct cggcacgacg ctcgccgccg aacggagctc
     4981 gctcgccgcg cgcaagcaat ggcttgccga tcacgtgcgc ctcgccgggc ggctcacgct
     5041 cgacagcggc gccgtgcacg cgctgacacg cgacggcaag agcctgctgc cgatcggcgt
     5101 cgtcgcggtg acgggcaatt tcggccgcgg cgaggtcgtc ggctgcatcg cgccggacgg
     5161 acgcgaagtc gcgcgcggcc tcgtcaatta cggcgcgcag gaggcggcgc gcatcgtgcg
     5221 ccggccgtct tcggaaatcg aatcggtgct gggctacgtc gacgagcccg agctcatcca
     5281 ccgcgacaac ctcgtgttgc tcgcatgagc ggcaccggcg tcgtcgagcg cgccaggcgc
     5341 atcctcgccg acccgcgcga cgaatggatc aagatcgcgg cggagcctgc gacggtgcaa
     5401 tcgctctaca cgaactggat catgatcctc gccgcgatcg ggccgatcgc ccttctgctg
     5461 tcgatgcatc cgctgcaggt cgcggtcgcg cagtacgtgc gctcgctgat cacgacgttc
     5521 gtgctggccc tcgtcgtcga cgcgctcgcg ccgtcgttcg gcggcagcaa ggatttcatc
     5581 gcgtcgctca agctcaccgc ctacagctac accgcggcat ggctcgccgg cgtcttcaac
     5641 atgctcggcg cgctcggcga cctcgtcatc ctgatcgcca cgctctatgc gctgtacacg
     5701 ttcctcgtgg gcgcgccggt gctgaaccgg tcgtcgcccg acaaggcggt gccgttcacg
     5761 ctcgtcgtgg tgctgtgcgc ggtcgtgctg ttcttcctcg cgcgcttcgc cttcaccggc
     5821 atgctggcgc cgccgatgca cgccggcatg ggcctggcaa tccgctgaca cgcgagtgtc
     5881 gctgtcgcac tcaactgcgc gacgtcgccg gattcgcgta cgacgcgggc atttcggcat
     5941 gcgcccaccc gtacagacgg tcacgccgcg tctgcggccg cccatgcagg aatcgctcga
     6001 gctccatcag cgcccggcgg tagacctcgc gcttgaaatc g
//
LOCUS       DASSVX010000113         3290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_187457, whole genome shotgun sequence.
ACCESSION   DASSVX010000113 DASSVX010000000
VERSION     DASSVX010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187457"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..832
                     /locus_tag="VFO53_05005"
     CDS             128..832
                     /locus_tag="VFO53_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875668.1"
                     /translation="MDTISQSLALALSMVAHLDARLTQIVGLSIAVSLASTLCSAVIG
                     LPLGAAIAIARFPGRDPVIVTLNAAMGLPPVVVGLLVYLLLSRAGPLGAFGLLFTPSA
                     MVIAQTILIVPIVAALTRQVVEDAWAEYREQLRSLDASWWQSSATLLWDTRWSLLTVL
                     LAGFGRAASEVGAVIIVGGNIDGVTRVMTTAIALETSKGDLALALGLGIVLLTLVMAL
                     NVGAHLTRQWARARFG"
     gene            825..1547
                     /locus_tag="VFO53_05010"
     CDS             825..1547
                     /locus_tag="VFO53_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8875669.1"
                     /translation="MGDAEIAEGLPIVACGVAVIRRGTRVLDAIDVAIEPRARTFILG
                     ANGAGKSTLLRVLHGLLAPDAGRVTWGSGTSRPPGQAMVFQRPVLLRRSAAGNVRHAL
                     ALRGVRGSEAQRRVDDALSRVGLGAIGARPARVLSGGEQQRVALARAWALEPEVLFLD
                     EPTASLDPPATRAVETIVEAIHARGTTIVMTTHSLGQAKRLADTVIFLSAGRVTECTD
                     ALQFFASPRSPEGRIFVEGERL"
     gene            1586..2371
                     /locus_tag="VFO53_05015"
     CDS             1586..2371
                     /locus_tag="VFO53_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8875670.1"
                     /translation="MLAAVCAGAAQPFIVVASTTSTEQSGLFAHLLPAFEKDTGIAVH
                     VVAVGTGQALDIGRRGDADVVFVHDKAAELKWLAEGNGVRRNPVMYNDFVLIGPKADP
                     AHVAGGKDIAAALKKIAQAKPAFVSRADKSGTYSAELRLWKDAGVDIATAKGSWYRET
                     GSGMGPALNTAAGLDAYLLADRGTWLNFHNRGNLEVVVEGDKRLFNQYGVMLVNPAKH
                     PQVKKNLGQKFIDWLVSPRGQQVIASYTIDGEQLFFPNAGSDS"
     gene            2368..>3290
                     /locus_tag="VFO53_05020"
     CDS             2368..>3290
                     /locus_tag="VFO53_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET8875671.1"
                     /translation="MTPIRRRTGLALGGGVLALFALALIALGAGRYPIPVRELAHVLW
                     TRLAGGSDAATADAIVWQIRLPRAGVAMLVGAALAAAGTAYQHLFRNPLVAPDTLGVS
                     SGAALGAVLGIVAGASFVVIEAAALAGGLCAVGVVMLIAARLEAHDPLVTLILTGIVV
                     ASLLGAAIALVKYLADPYNELPAITFWLMGSLASASAAEVASLLPAVALALVVLFALA
                     WRVNLLALPEDEARALGVSTRRLRGVVIAAATLATAASVAVSGVIGWVGLVVPHMARL
                     VVGPEFSRLLPAAALFGAAFMLVIDTISRTVA"
BASE COUNT          458 a         1091 c         1156 g          585 t
ORIGIN      
        1 gtgctgatcg ccggctggct cggcacgcgc gagacggtgc gcgagccgcc gctcgccgtg
       61 ctgcgcgagc tcgcctgatg taagaattgc ataggaatgc agttccgcgg cacaatatgc
      121 agtccagatg gacacgattt cgcaaagcct ggcactcgcg ctgtcgatgg tcgcccacct
      181 cgatgcgcgg ctgacacaga tcgtcggact gtcgatcgcg gtcagcctcg cctcgacgct
      241 ctgctcggcg gtgatcgggc tgccgctggg cgcggcgatc gccatcgcac gctttcccgg
      301 acgcgatccc gtcatcgtga cgttgaatgc ggcgatgggc ctgccgccgg tcgtcgtcgg
      361 cctgctcgtc tatctgctgc tgtcgcgcgc gggtccgctc ggcgcgttcg gcctgctgtt
      421 cacgccgagc gccatggtga tcgcccagac gatcctgatc gttccgatcg tcgccgcact
      481 cacgcgccag gtcgtcgagg acgcgtgggc cgagtaccgc gagcagctgc gcagcctcga
      541 cgcgtcgtgg tggcagtcga gcgcgacgct tttgtgggac acgcgctggt cgcttctgac
      601 ggtgctgctc gcgggcttcg gacgggcggc gtcggaagtg ggcgccgtga tcatcgtcgg
      661 cggcaacatc gacggcgtga cgcgcgtcat gacgaccgcg atcgcgctcg aaacgagcaa
      721 gggcgatctc gcgctcgccc tcggcctcgg catcgtcctg ctgacgctgg tgatggcgct
      781 caacgtcggc gcgcatctca cgcggcaatg ggcgagggcg cgctttgggt gatgcggaga
      841 tcgccgaagg gttgccgatc gtcgcttgcg gcgtcgccgt catccgacgc ggcacgcgcg
      901 tgctcgatgc aatcgacgtc gcgatcgagc cacgcgcgcg cacgttcatc ctgggtgcca
      961 acggcgccgg caagagcacg ctgctgcgcg tcctgcacgg actgctcgcg cccgatgccg
     1021 gccgcgtgac gtggggaagc ggcacgtcgc ggccgccggg gcaggcgatg gtgttccagc
     1081 ggccggtgtt gctgcgccgc tcggcggcgg gcaacgtgcg tcatgcgctc gcgcttcgcg
     1141 gcgtgcgggg aagcgaggcg caacggcgtg tcgatgatgc gctatcgcgc gtgggtctcg
     1201 gtgcgatcgg cgcgcgcccc gcgcgcgtgc tgtccggcgg cgagcagcag cgcgtggcac
     1261 tcgcacgggc gtgggcactc gagcccgaag tcctgttcct cgacgagccg accgcgagcc
     1321 tcgatccgcc cgcgacgcgc gcggtcgaga ccatcgtcga agccattcac gcccggggaa
     1381 cgacgatcgt gatgacgacg cacagcctcg gacaggcgaa gcgtcttgcc gatacggtga
     1441 tcttcctgtc cgcgggccgg gtgaccgaat gcacggacgc gttgcaattt ttcgcgtcac
     1501 cacgttcgcc cgaaggaaga atctttgtcg aaggagagcg cttgtgatga aaacgtggag
     1561 caggcatttc gtgttggcgc tcggcatgct cgccgcggtt tgcgcgggtg ccgcgcaacc
     1621 gttcatcgtc gttgcgtcga cgacgtcgac cgagcagtcg gggctgtttg cgcatctttt
     1681 gccggcgttc gaaaaggaca ccggcatcgc cgttcacgtg gtcgcggtcg gcaccggaca
     1741 ggcgctcgac atcggccgcc gcggcgatgc cgacgtcgtc ttcgtgcacg acaaggccgc
     1801 cgagctcaaa tggcttgccg aaggcaacgg tgtcaggcgt aatcccgtca tgtacaacga
     1861 cttcgtgctg atcggaccca aagccgatcc ggcgcacgtc gccggcggca aggacatcgc
     1921 ggccgcattg aaaaagatcg cgcaggcgaa gccggcgttc gtgtcgcggg ccgacaagag
     1981 cggcacctat tcggccgagc tgcggctgtg gaaggatgcg ggcgtcgaca tcgctacggc
     2041 gaaggggtcg tggtaccgcg aaaccggatc gggcatgggt ccggcgctca atacggcggc
     2101 cggcctcgac gcgtatctcc ttgccgatcg cggcacgtgg ctcaacttcc acaaccgcgg
     2161 caatctcgaa gtcgtcgtcg agggcgacaa gcggctcttc aaccagtatg gcgtgatgct
     2221 ggtgaatccc gcgaagcatc cgcaagtcaa aaagaatctc ggccagaagt tcatcgactg
     2281 gctcgtgtcg ccgcgcggac agcaggtgat cgcgagctat acgatcgacg gcgaacaatt
     2341 gttctttccc aacgccggct cggattcgtg acgccgatac gccgccgcac cgggctcgcg
     2401 ctcggcggcg gcgtgcttgc gcttttcgcc cttgcgctga tcgcgctcgg cgcgggccgc
     2461 tatccgattc cggtgcgtga gctcgcgcac gtgctgtgga cgcggctcgc cggcggtagc
     2521 gacgcggcga ccgccgatgc gatcgtgtgg cagattcgcc tgcctcgcgc cggtgtggcc
     2581 atgctcgtcg gggcggcgct cgcggccgcg ggcaccgcgt accagcacct ctttcgcaat
     2641 ccgctcgttg cgcccgacac gctcggcgtt tcatccggtg cagcgctcgg cgccgtgctc
     2701 ggcatcgttg cgggcgcaag cttcgtcgtc atcgaggccg ccgcgctcgc tggagggctt
     2761 tgcgccgtcg gcgtcgtcat gctgatcgcc gcgcgtctcg aggcgcacga tccgctcgtg
     2821 acgctgatcc tcaccgggat cgtcgtggcg agcctgctcg gcgcggcgat tgcgctcgtc
     2881 aaatacctcg ccgatccgta caacgaattg cctgcgatca ccttctggct gatgggaagc
     2941 cttgcatccg cgtccgcggc agaggtcgcg agcctgctgc cggccgtagc gctcgcgctc
     3001 gtcgtgctgt tcgcgctcgc gtggcgggtc aacctgctcg cgttgccgga ggacgaggcg
     3061 cgggcgctcg gcgtgagcac gaggcgattg cgcggcgtcg tcatcgctgc ggcgacgctc
     3121 gcgaccgcgg caagcgtcgc cgtttccggc gtcatcggct gggtgggcct cgtggtgccg
     3181 cacatggcgc ggcttgtcgt cgggcccgag ttctcgcgcc tgctgccggc cgcggcgctg
     3241 ttcggcgccg ccttcatgct cgtcatcgac acgatttcac gtaccgtggc
//
LOCUS       DASSVX010000114        18608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_820709, whole genome shotgun sequence.
ACCESSION   DASSVX010000114 DASSVX010000000
VERSION     DASSVX010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 18608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18608
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820709"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFO53_05025"
     CDS             <1..207
                     /locus_tag="VFO53_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HET8875672.1"
                     /translation="PLATTLVAISAGAVFMGANTYIGNAPNFMVKSIAESRGVKMPTF
                     FGYLGWSLLVLVPVYALLTLLFFA"
     gene            275..973
                     /locus_tag="VFO53_05030"
     CDS             275..973
                     /locus_tag="VFO53_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET8875673.1"
                     /translation="MSALSKPLDAVDDPGLVRFVQRGDTQAFGVLMRRYNRRLYRTAR
                     AILRDDAAAEDAVQEGYVAAWRHIGEFRGDAQIATWLTRIVVNQALQALRRTRREQVV
                     VLFEEPPDERNPDDEIAESLSPRAPETMMLRAEMRRLIESKIDDLPEGYRTVFVLREV
                     EDMTVEETAAALGIPAATVRSRLFRAKARLRESLAEELDTAVQQVFGFDGERCDRIVR
                     AVLARISDPAHPVD"
     gene            1022..1543
                     /locus_tag="VFO53_05035"
     CDS             1022..1543
                     /locus_tag="VFO53_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET8875674.1"
                     /translation="MKRIPTMVAAALLSATFVAYAQGGPNDAQIAAIVVTANQVDIDA
                     GKLAESKAKNKDVKAFGKMMVTDHTGVNKAAKDLVTKLHVKPEDNPTAESLKKGGQDN
                     VAHLKTLKGDAFDKAYIEHEIVYHQTVIDAMDKTLIPSASNAELKALLVKVRPAFVAH
                     LDHAKQVQAEIGK"
     gene            1545..1877
                     /locus_tag="VFO53_05040"
     CDS             1545..1877
                     /locus_tag="VFO53_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HET8875675.1"
                     /translation="MARHGARALAALCAVALALVATGSEAAGTVHKVTIDGFAFHPPS
                     LTVKQGDVVEWHNTDPVPHTATAKDAGLDSGDIAANATYRFTAKKKGRFAYLCTLHTI
                     MKGELIVE"
     gene            1942..2604
                     /locus_tag="VFO53_05045"
     CDS             1942..2604
                     /locus_tag="VFO53_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8875676.1"
                     /translation="MPPEPSDTRTRLVDTAMRLFMEKGYGSTSVADVLKEAGANSGSL
                     YHFFPGKQDLLLAVLDRYREGIGPQLLEPAWAGIDDPIERVFALLARYRQGIVMTDCT
                     YGCPIGSLALELHEPDPAVRAALAANFDAWTAAVLGCLDAAGSMLPADLDRHALAQFV
                     LTTMEGAVMQARTHRDVAYFDASIGELRRYFDCLRNGRRRAVPKKRRLRSAQRLRKES
                     HP"
     gene            2613..3104
                     /locus_tag="VFO53_05050"
     CDS             2613..3104
                     /locus_tag="VFO53_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875677.1"
                     /translation="MRYLLAALLLLPAAVLAEPIAPLQPMSFLAGHCWKGQFPDGKQT
                     DEHCFTWLYDGQALRDTHTVRAAGRPDYIGETTYYWDAEQKHVAFLYIENLGGVSRGT
                     MEPVANGLVFPPARYVAGNESMTYRVRWTRQGDDAYEALSEMQAKDGWTTMFKLLLKR
                     MPS"
     gene            complement(3159..4316)
                     /locus_tag="VFO53_05055"
     CDS             complement(3159..4316)
                     /locus_tag="VFO53_05055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET8875678.1"
                     /translation="MKASLPLPGADARAHSARVLAQVRDEIDRAGGFIAFSRYMELVL
                     YAPALGYYVAGARKFGEAGDFVTGPELTPLYGAAIARQLAPILEESRARDIVEFGAGS
                     GALAASLLDALALHGASPSRYRILEVSPQLRAAQRSTIATCAPAALTRVEWIDRLPDA
                     IDGVVVMNEVLDAIAPRLVARRDGAWFERGVTLVDDALAWDERPLDDARLEAVARARF
                     PADGDYTSELNPAAEALVTTIGRRIASGALLIVDYGFPRHEYYHPQRNTGTLVGHYRH
                     RVHADPFVWPGLSDLTSHVDFTAMAEAAERAGLQVAGFATQASFLLGCGILDLLRDVG
                     APETSAYLRAASAVQKLLSPAEMGELFKVLLLARGDAAWRSLALADMTHRL"
     gene            4379..5116
                     /locus_tag="VFO53_05060"
     CDS             4379..5116
                     /locus_tag="VFO53_05060"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HET8875679.1"
                     /translation="MEGRIVLITGGAKRVGASICRRLHRAGASIMLHFRASAGEARLL
                     QAELNHVRPHSVALIQADLLDVAKLPSLVEQTVQTFGRLDALVNNASTFYQTTIGDIG
                     VDDWNNLIGTNLQAPLFLAQAAAPALKKAQGAIINITDIHAERPLKSYVVYSVAKAGL
                     VGLTRSLARELAPEVRVNAVAPGPILWPDEEVFDELARQRIISHTPLRREGSPDDVAK
                     AVHYLLAEAPYVTGETINVDGGRHIAL"
     gene            complement(5159..5848)
                     /locus_tag="VFO53_05065"
     CDS             complement(5159..5848)
                     /locus_tag="VFO53_05065"
                     /inference="COORDINATES: protein motif:HMM:NF016627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HET8875680.1"
                     /translation="MTLSRRDLEEGRMREVYVEALAEGHALTDEQLSESLTATLHAKP
                     RGAGWWVFAYGSLLWNPIFPFAEARRASVHGLHRRFCLWSLASRGRPSAPGLVLGLDH
                     GGSCRGVVYRLPSPCAIDELHLLWRREMVTGAYAPRWLHADCEGRPIMALGFVVKREH
                     AQYAGHLTIEAQADVIAHACGAFGTSADYLERTRVSLATHGVIDLYLERLAGRVAAKS
                     RVRDDFPDLKR"
     gene            complement(5877..7115)
                     /gene="hemW"
                     /locus_tag="VFO53_05070"
     CDS             complement(5877..7115)
                     /gene="hemW"
                     /locus_tag="VFO53_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HET8875681.1"
                     /translation="MEVDAATIGTVDADSLRGPRFAALPPLALYVHMPWCVRKCPYCD
                     FNSHEWRSRNGEAAPAATAPDALPEDAYVAALIADVESALPSVWGRKIGSLFIGGGTP
                     SLFSAASIERLLSALRARLPLLPDAEITLEANPGTFEREKFAGYFAAGVNRLSLGVQS
                     FDDRFLRALGRIHGADEARRAAESALMIFGNVNLDLMFALPNQTPDDARRDIAAALSF
                     APPHLSFYHLTLEPNTLFHRHPPPLPDDDAAADIEEGVLDMLAHAGYAHYETSAHARP
                     GFACRHNVNYWRFGDYLGVGAGAHSKLSFPDRVVRQMRWKQPKQYLEAVARGTPIQET
                     HDVSRSDLPFEFMLNALRLTDGVPTSMFAERTGLSLSAAAQPIAEATRRGLLEPDPAV
                     LKPTPRGRRFLNDLQQLFLP"
     gene            complement(7105..7707)
                     /gene="rdgB"
                     /locus_tag="VFO53_05075"
     CDS             complement(7105..7707)
                     /gene="rdgB"
                     /locus_tag="VFO53_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HET8875682.1"
                     /translation="MERLVLASGNEGKLREFARLLAPLGLEVIPQAELGIAPAGEPHV
                     TFIENALAKARHASAQSGLPALADDSGVCVDALGGAPGVRSARYAGEPVSDARNNAAL
                     ITALRDVADRRAHYYCVLVLVRCAEDPQPVIAEGIWHGTIVDTPRGSGGFGYDPHFQD
                     AITGRTGAELPLPRKNALSHRGKAMRRLMARLEILQRDGG"
     gene            complement(7707..8456)
                     /gene="rph"
                     /locus_tag="VFO53_05080"
     CDS             complement(7707..8456)
                     /gene="rph"
                     /locus_tag="VFO53_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET8875683.1"
                     /translation="MATIEPSRQKQARAGGRRADELRTVRIARHFTKHAEGSVLIEAG
                     QTRVLCTASVEEGVPLFLKGKGQGWLTAEYGMLPRATNTRTRREAAEGRQSGRTQEIQ
                     RLIGRSLRAVIDLAALGERTIRIDCDVLQADGGTRCASITGAYVALADAIAWCRARRT
                     LAGEPLRDRVAAVSVGVVAGTPLLDLDYAEDSGCDTDMNVVMTGAGGFVEVQGTAERE
                     PFSRDQFDALLALAQQGIARLHDAQREALSA"
     gene            complement(8479..9465)
                     /locus_tag="VFO53_05085"
     CDS             complement(8479..9465)
                     /locus_tag="VFO53_05085"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET8875684.1"
                     /translation="MVLSAAPIVVTGAGGFIGAACCRRMQARGQPVRRFVHAHAAPDA
                     SGDAVAVDLARVHADPLTQALRGAFAVVHLAGRAHVMNEFAPDAEAAYRLANVDATER
                     LALAAVAAGVHRFVFASTVKVNGESTLPGRPFEPGDPAAPHDAYARSKLAGELALARA
                     ASGTAMVATTLRLPLVYGRGARGNFRRLVDAVAARRFLPLGAIDNRRSLVALDNLLDA
                     IDAVLDAPSPVAGTHFVADATAISTPDLVRAIAAALGVAPRLVPVPVTVLRVAGALSG
                     RSDAVTRLAGSLEVDTASLSHATRWRPRPVSIDAASVGAAAAGDMSEHASPL"
     gene            complement(9456..10298)
                     /locus_tag="VFO53_05090"
     CDS             complement(9456..10298)
                     /locus_tag="VFO53_05090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8875685.1"
                     /translation="MLPSSLQISIVTYLPDLPLLTRTLDTLAIAIDVAQQAQALRTVH
                     LALIDNSSDRGIAERLIELGRARFADARVHVVFLHGHANIGFGAAHNLVLHGTGADYH
                     LVLNPDAELAADALVNAIRWLDAHPGVGALAPTVTDGNGNRQYLCKRYPAVFDLLLRG
                     FAPGFVQRLFARRLARYEMRDIVDAVPPREAIGVPVMSGAFLLARREAIDRTGGFDPR
                     FFLYFEDYDWTMRLRDVAQTAFVPSVNVVHHGGRAARKGLRHVRWFLASGVRFYARHG
                     WKWF"
     gene            10372..11517
                     /locus_tag="VFO53_05095"
     CDS             10372..11517
                     /locus_tag="VFO53_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HET8875686.1"
                     /translation="MSTPMPPPAADTATHAAAAVETRYPAERLQAFAVALLAAAGVRD
                     DVAHDVASVLLDGDLMGHTTHGLQLLPGYLDALAGGGMRKTGEPVTTSSYPASRTWDG
                     QRLPGPWLTLRALDDARRMASTHGTGTVVIRRSHHIGCLASYAKRAAERGVMTIVCCI
                     DPATHSVAPFGAITSVFTPNPLAAGIPTSGEPILLDISASYTTNGMTMRLHRNGQELP
                     HPWVQDARGNPTRDPAVLFNEPKGTLLPLGGLDAGHKGFALALLIEAMTAGLAGHGRA
                     DPAEGWGATVFVQVLDPAAFGGLDAFTRQMDWLVRACHEATPRPDGPPVRLPGERALR
                     LYREQLANGVALYPEIMPALAPWAVKLGVPPPTATPSPPPPRPPSSD"
     gene            complement(11468..13276)
                     /locus_tag="VFO53_05100"
     CDS             complement(11468..13276)
                     /locus_tag="VFO53_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET8875687.1"
                     /translation="MSLVLLVAASAIAQPKPRIEKAADLPRFSYPIEGRVEDVIGDDA
                     KFKRFASAVRRDAESVLSGYEIDDRATLRQLEGELAQLDYLDGNDDGALRRAAHVREL
                     QDKPADKLMSGLQLRAMIAAQRKSGDRTSDAYRQETGRLIAADLGQMPYDVVQNEVRE
                     AKASAEIMSEALTLGYVRNVIQPTVDKAGALSSDLAPVVISAKYRLVASLPLKATLVA
                     TYSSYLAAHHVDKPDIWAARDVVLPPGKRYAPVNVGVWDSGVDTSLFGKRVVDDAGKP
                     AVIAFDRYTKPATGTLQPIPAVLKDQIPQMKSRLKGFSDLRSNIDSPEASEVKQYLST
                     LKPDEYKAAIESLDLAGNWMHGTHVAGITLAGNPYARLVVGRIEFDWHLLPDPCPTKA
                     LALQDAKNQQAYVDFFRKHRVRVVNMSWGGSIKAEEEQLELCNIGKTPDERKKIARDY
                     FDIQKDALTKAFASAPDILFVTAAGNSNEDASFTEDIPAGISAPNLITVGAVDKAGDE
                     ASFTSYGPTVVVDANGYQVDSVIPGGDKLAESGTSMASPQVANLAAKILAVNPKLAPA
                     QVIALIRDTADKTPDGRRTLINPKKAVAAAEAKASP"
     gene            complement(13684..14604)
                     /gene="prmC"
                     /locus_tag="VFO53_05105"
     CDS             complement(13684..14604)
                     /gene="prmC"
                     /locus_tag="VFO53_05105"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET8875688.1"
                     /translation="MSVARPPRTPACNRASEDHSTVRQALVQAGLAPIDAQVLLAHVL
                     GTDRAWLVAHATDPLARRDAEAFFTLARRRRDGEPVAYLTGEREFWGLSLAVDRSVLI
                     PRPETETLVECALRSLPSDRPVRVLDIGTGSGAIAVAIARERPQAQVLATDVSAAALV
                     VATRNAARMKLGNVRLLQSNWYERLSGGESVFDLIVSNPPYVADADPHLAEGDVRFEP
                     VTALRGGRDGLAALHVVIAGAPAWLAPGGGLIVEHGYDQADAVRALMRAAVLADAGVF
                     RDLAGIARVSAATKAVAPRRIPLTPLQSES"
     gene            complement(14601..15215)
                     /locus_tag="VFO53_05110"
     CDS             complement(14601..15215)
                     /locus_tag="VFO53_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8875689.1"
                     /translation="MNGEGLPPATSRVSPGTSAAANPRRLAIPWCVTKRLALSEITAD
                     DGNELHELDTDPRVMQYIGSGRLSTREQIDDALRRIPRAYLLYPGLGTWRATRRDNGD
                     FVGWFALKYIPGTAEVEVGYRLRFGAWGRGLATEGARALVRYGFDELGLYRILGVTHP
                     DNLASQRVLMKAGMVDAGWGRYYNRPVRVFERVRDAAWRPQWRV"
     gene            complement(15212..16294)
                     /gene="prfA"
                     /locus_tag="VFO53_05115"
     CDS             complement(15212..16294)
                     /gene="prfA"
                     /locus_tag="VFO53_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET8875690.1"
                     /translation="MKLSLRTRLDQLSARLMELDALLAAEDAARDLDRFRGLARERAE
                     IDPVVARFGDFLRAEGDLAAAVDLAHDPQMRDYADEERRAAEARLVVLEGELQAMLLP
                     KDPSDERNVFIEIRAGTGGDESALFAADLFRMYARYAERQRWRVEIVSESASDLGGYR
                     EIIGRVVGAGAYARLKFESGGHRVQRVPETEAQGRIHTSACTVAVLPEADPVADVTIN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHVPTGIVVECQDDRSQHRNRAQAMSVLAS
                     RLVDKERRERQQKEAAHRKSLIGSGDRSERIRTYNFPQGRVTDHRINLTLYKIDAIMQ
                     GDLDELIGALAQEFQAEQLADLATHA"
     gene            complement(16410..17672)
                     /gene="hemA"
                     /locus_tag="VFO53_05120"
     CDS             complement(16410..17672)
                     /gene="hemA"
                     /locus_tag="VFO53_05120"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET8875691.1"
                     /translation="MSLYTLGLNHTTAPLDLRERVAFAPDALSGALRELTSGRVVKEA
                     AILSTCNRTEVYFHGGEPAPVARWLEQAHALAPSSLSPYVYTLNGDRAVSHAFRVASG
                     LDSMVLGEPQILGQMKQAVRQAEAAGSLGLVLNRLFQRTFAVAKDVRTQTDIGSASIS
                     MAAAAVKLAERIFPSLADQCLLLVGAGEMIELAATHFAARKPRSITVANRTLERGHQL
                     AHRFGAEAITLTELNERLAQFDVIITCTASSLPIIGKGTLERVVKRRKHAPVLIVDLG
                     VPRDVEAEAATLDDVFLYSVDDLAEIVKDNLSIRREAVVQAEQMIAEQTAHFLRWLQG
                     RSVVPTIAALASHHDALRKHELDRAQRMLAAGAPANEVLEALARSLTSKFLHPPLAAL
                     NAAGDAERAELIALFSRVYGCAEPPPEG"
     gene            17827..>18608
                     /locus_tag="VFO53_05125"
     CDS             17827..>18608
                     /locus_tag="VFO53_05125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET8875692.1"
                     /translation="MNIETTHDEKIGPDRAPASFARRCWPLVALGLVGIASLPLVMLP
                     TLRLLIRDGHAPGMSLPTLAALTLIQPTLLLIAAAAIGAALAPRLALSSHVAKVNVRS
                     VVAAEVRLALVCGVVTGIAVVALDIALFHAHSAATSASARTIIDGLIGGALYGGITEE
                     IMLRWGLMSLVVWAGARLFARGAARPAPGVYRVAIAVVALLFAAGHLPAAMAIAPLTT
                     ALVFRILLLNSLAGLVFGWLFWRRSLECAMLAHASVHVVFAIG"
BASE COUNT         2997 a         6394 c         6356 g         2861 t
ORIGIN      
        1 ccgctcgcga cgacgcttgt cgccatctcc gccggcgccg tattcatggg agcgaacacc
       61 tacatcggca acgccccgaa tttcatggtc aaatccatcg ccgagtcgcg cggcgtgaaa
      121 atgccgacct ttttcggcta tctcggctgg tcgcttctcg tcctcgtgcc cgtctacgca
      181 ctgctgacgc tgctgttctt tgcctgacga cgtaaggcgg gaacgccgcc ggtgccgttt
      241 gcatccaatg ggcaccttac cgaagatgcc cgtcatgtct gcgctctcga aaccgctcga
      301 cgccgtcgac gacccggggc tcgtccgctt cgtccagcgt ggcgacaccc aggcgttcgg
      361 cgtgctgatg cgccgctaca accggcgcct ctaccgcacc gcgcgggcga tcctccgcga
      421 cgacgccgcg gccgaagacg cggtgcagga aggctatgtc gccgcgtggc gccacatcgg
      481 cgagttccgc ggcgacgcgc agatcgcgac ctggctcacc cggatcgtcg tcaaccaggc
      541 gctgcaggcg ctacgcagga cgcgccgcga gcaggtcgtg gtgctgttcg aggagccacc
      601 cgacgagcgc aaccccgacg acgagatcgc cgagtccctg tcgccacgcg cgccggagac
      661 catgatgctg cgtgccgaga tgcgccgact gatcgaaagc aagatcgacg acctgcccga
      721 gggctatcgc acggtgttcg tgctgcgcga agtcgaggac atgacggtcg aggaaacggc
      781 cgccgcgcta ggcattccgg ccgccacggt gcgaagccgc ctgttccggg cgaaggcgcg
      841 cctgcgcgag tcgctggccg aggagctcga cacggcggtg cagcaagtgt tcggcttcga
      901 cggcgagcgc tgcgaccgca ttgtccgggc cgtgctggcg cggatttcgg accctgcgca
      961 tccagtggac tgattcgtcc gtaacttacc ctgcattctc cccattgaag aaggagcaac
     1021 gatgaaacgc attcccacca tggtagcggc ggccctgctt tccgccacgt tcgtagccta
     1081 cgcacagggc ggcccgaacg acgcgcagat cgccgcgatc gtcgtcaccg ccaaccaggt
     1141 cgacatcgac gccggcaagc tcgccgaatc gaaggcgaag aacaaggacg tcaaggcgtt
     1201 cggaaagatg atggtcaccg accataccgg cgtcaacaag gcggcaaagg acctcgtgac
     1261 gaagctgcac gtgaagcccg aggacaaccc gaccgccgaa agcctcaaga agggcggcca
     1321 ggacaacgtc gcgcacctca agacgctgaa gggcgacgcg ttcgacaagg cgtacatcga
     1381 gcacgagatc gtctatcacc agacggtcat cgacgcgatg gacaagacgc tgatcccgag
     1441 tgcttcgaat gccgagctca aggcactgct cgtcaaggtg cgccccgcgt tcgtcgccca
     1501 cctcgaccac gcgaagcagg tgcaggccga gatcgggaag taacatggct cgtcacggcg
     1561 cccgcgcgct ggcggcgctc tgcgccgtcg cgctggcgct cgtcgcgacg ggaagcgaag
     1621 ccgccggcac cgtgcacaag gtgaccatcg acggcttcgc gtttcacccg ccgtcgctca
     1681 ccgtcaagca gggcgacgtc gtcgagtggc acaacaccga cccggtgccg cacacggcca
     1741 ccgcgaagga cgccgggctc gattccggcg acatcgccgc caacgccacc taccgcttca
     1801 ccgcgaagaa gaaggggcgc ttcgcgtacc tctgcacgct gcacacaatc atgaagggcg
     1861 agctgatcgt cgagtagcgc cggctcgttg catgttcggc atggccgtgc tagcctcttg
     1921 gttgatcact caaccaagcc gatgcctccc gaaccgtccg atacacggac gcgcctcgtc
     1981 gacaccgcca tgcgcctctt catggagaag ggttacggca gcacgagcgt cgccgacgtc
     2041 ctgaaggagg ccggtgcgaa cagcggcagc ctttatcact tcttcccggg aaagcaggac
     2101 ctgctgctcg ccgtgctcga tcgctaccgc gaaggcatcg gtccgcaact cctcgagccc
     2161 gcatgggccg gcatcgacga tccgatcgaa cgcgtgttcg cgctgctcgc gcgctaccgg
     2221 caggggatcg tcatgaccga ttgcacgtac ggctgcccga tcggcagcct cgccctcgag
     2281 ttgcacgagc ccgatcccgc cgtgcgcgcg gcgctagccg cgaacttcga cgcgtggacc
     2341 gccgcggttc tcggctgcct cgacgccgcg ggcagcatgc ttccggccga cctcgaccgg
     2401 cacgcgctcg cgcaattcgt gctgacgacg atggaaggcg cggtgatgca ggcgcggacg
     2461 catcgcgacg tcgcctactt cgacgcgtcg atcggcgagc ttcgccgcta tttcgattgc
     2521 cttcggaacg gccgccggcg cgccgtgccg aaaaagcgtc ggttgcgttc cgcccaacgg
     2581 ctccgcaagg agtcccaccc gtgaggtcga ccatgcgcta cctgcttgcc gcattgctgt
     2641 tgcttcccgc tgccgtcctt gcggagccga ttgcaccgct gcagccgatg tcgtttctcg
     2701 ctggccactg ctggaagggg cagttcccgg acggcaagca gaccgacgag cactgcttca
     2761 cctggctcta cgacggccag gcactgcgcg acacgcacac ggtgcgcgcc gcgggccgcc
     2821 ccgactacat cggcgagacg acgtactact gggacgccga acaaaagcat gtcgcgtttc
     2881 tctatatcga gaacctcggc ggcgtcagcc gcggcacgat ggaacccgtc gcgaacggcc
     2941 tcgtgtttcc gcccgcgcga tacgtcgccg gcaacgagtc gatgacctac cgcgtgcgct
     3001 ggacgcgcca gggcgacgac gcgtacgagg cgttgagcga aatgcaggcg aaggatggct
     3061 ggacgacgat gttcaagctg ctgctgaagc gcatgccgtc gtgacgccat gccgcttcgg
     3121 ggcgcggcga acgccggctg tcgcagcggc cggcggcgtc acagccgatg cgtcatgtcg
     3181 gcgagcgcga gcgagcgcca ggcggcgtca ccgcgcgcca gcagcagcac cttgaagagc
     3241 tcgcccattt cggcggggga caggagcttc tggaccgcgc ttgccgcacg caggtaagcg
     3301 gacgtttcgg gcgcgccgac gtcgcgcagc aggtcgagga tgccgcagcc gagcaggaac
     3361 gacgcctgcg tcgcgaatcc cgcgacctgc aaccctgcac gctccgccgc ctcggccatt
     3421 gcggtgaaat cgacgtggct cgtgagatcg gacagccccg gccacacgaa cggatcggcg
     3481 tgcacgcgat gccggtaatg cccgacgagc gttccggtgt ttcgctgcgg gtggtagtac
     3541 tcgtggcgcg gaaaaccata gtcgacgatc agaagtgcgc ccgatgcgat gcgccggccg
     3601 atcgtcgtca ccagcgcctc ggccgcggga ttcagttcgc tcgtgtagtc gccgtccgcc
     3661 ggaaagcgcg cacgcgcgac ggcttcgagc cgcgcgtcgt cgagcgggcg ctcgtcccac
     3721 gcgagcgcat cgtcgactag cgtgacgccg cgctcgaacc acgcaccatc gcggcgcgcg
     3781 acgagccgcg gcgcaatcgc gtcgagcacc tcgttcatca cgacgacgcc gtcgatcgca
     3841 tcgggaagcc ggtcgatcca ttcgacgcgc gtaagcgcgg ccggcgcgca cgtcgcaatc
     3901 gtcgatcgct gcgcggcgcg aagctgcgga ctcacctcga gaatgcggta gcgcgaaggc
     3961 gatgcgccgt gcaacgcgag cgcatccagt aacgacgccg cgagtgcacc gctgccggcg
     4021 ccgaactcga cgatgtcgcg cgccctcgac tcctcgagga tcggcgccag ctggcgcgcg
     4081 atcgcggcgc cgtaaagcgg cgtcagctcg ggaccggtga cgaaatcgcc cgcctcgccg
     4141 aacttgcgcg cgccggcgac gtaatagccg agcgccggcg catacagcac gagctccatg
     4201 tagcgcgaga acgcgatgaa tccgccggca cggtcgatct cgtcgcgaac ctgggcgagg
     4261 acgcgcgcac tgtgcgcgcg tgcgtcggcg ccgggaagcg gcagcgaggc tttcaacggc
     4321 gtggatccgt tgaaaactgt gctagattgc cccgtcccat cgacgcgcaa ggctttgcgt
     4381 ggaaggcagg atcgtactga tcaccggcgg cgccaagcgg gtcggtgcca gcatctgccg
     4441 gcggctgcac cgcgcgggcg cgtcgatcat gctgcatttt cgcgcgtcgg cgggcgaggc
     4501 gcgcctgctg caggccgagc tcaaccacgt tcggccgcat tcggtcgcgc tgatccaggc
     4561 cgaccttctc gacgtcgcga agctgccgtc gctggtcgag cagactgtgc agacgttcgg
     4621 ccggctcgac gcgctcgtca acaacgcctc gacgttctac cagaccacga tcggcgacat
     4681 cggtgtcgac gactggaaca acctgatcgg caccaatctg caggcgccgc tgtttctcgc
     4741 gcaggccgca gcacctgcgc tcaagaaggc acaaggcgcc atcatcaaca tcaccgacat
     4801 ccacgccgag cgcccgctca agagctacgt cgtctattcg gtggcgaagg cgggactcgt
     4861 cgggctcacg cgctcgctgg cacgcgagct tgcgcccgag gtgcgcgtga atgccgtcgc
     4921 ccccggaccc atcctgtggc ccgacgagga agtgttcgac gagctcgcgc gccagcgcat
     4981 catctcgcat acgccgctgc ggcgcgaagg ctcgcccgac gatgtcgcga aggccgtgca
     5041 ctacctgctc gccgaggcgc cgtacgtcac cggcgagacg atcaacgtcg acggcggaag
     5101 gcacatcgcg ctctaaaagt agggtcagag acgactttcc ggtcggcctc aacgagcttc
     5161 atcgcttcaa atccggaaag tcgtctctga ccctactttt agcggccacg cgtcccgcga
     5221 gacgctccag gtacagatcg atgacgccgt gggttgcgag cgacacgcgc gtgcgctcga
     5281 gataatcggc cgacgttccg aacgcgccgc acgcatgcgc gatcacgtcc gcctgcgcct
     5341 cgatcgtcag atggcccgcg tactgcgcat gctcgcgctt gacgacgaag ccgagggcca
     5401 tgatcggccg cccctcgcaa tcggcgtgca gccagcgcgg cgcataggca ccggtgacca
     5461 tctcgcggcg ccatagaaga tgcagctcgt cgatcgcgca cggcgaaggc agccgataca
     5521 ccacgccgcg gcacgagccg ccatgatcga gccccagcac gaggcccggc gcgctgggtc
     5581 tcccgcgcga cgcgagcgac cacaggcaga agcggcgatg cagcccgtgc accgaggcac
     5641 ggcgcgcttc ggcgaacggg aagatcggat tccacaagag cgacccgtac gcgaacaccc
     5701 accagccggc acccctgggc ttcgcatgca atgtcgcggt gagcgattcc gacagttgct
     5761 cgtcggtaag cgcgtgacct tcggcgagcg cctcgacgta cacctcgcgc atgcggcctt
     5821 cctcgaggtc gcggcgggac agcgtcatcg ggccgcccga gcgtgctgcg cgcgcttcac
     5881 ggcagaaaca actgctgcag gtcgttcagg aagcgccgcc cccgcggcgt cggcttcagc
     5941 acggcggggt cgggctcgag caatccgcgc cgcgtcgcct cggcgatcgg ttgcgcggcg
     6001 gcggacagcg acagccccgt gcgctcggcg aacatcgacg tcggtacgcc gtcggtgagc
     6061 cgcagcgcgt tcagcatgaa ttcgaacggc aggtcgctcc gggaaacgtc gtgcgtttcc
     6121 tggatcggcg tgccgcgcgc aaccgcctcg agatactgct tcggctgctt ccagcgcatt
     6181 tgccgcacga cgcgatcggg aaacgaaagc ttcgaatgcg ccccggcacc gacgccgaga
     6241 taatcgccga agcgccagta gttgacgttg tgccggcacg cgaatcccgg cctcgcatgc
     6301 gccgacgtct cgtagtgcgc gtagcccgca tgggcaagca tgtcgagcac gccttcctcg
     6361 atatcggccg ccgcgtcgtc gtcgggcagc ggcggggggt gccgatggaa aagcgtgttc
     6421 ggctcgagcg tcaggtgata gaacgacagg tgcggcggcg cgaacgacag cgcggcggcg
     6481 atatcgcggc gcgcatcgtc cggcgtctgg ttcggcagcg cgaacatcag gtcgagattg
     6541 acgttgccga agatcatcag cgccgattcc gcggcgcggc gcgcttcgtc ggcgccgtgg
     6601 atgcgtccga gtgcgcgcag gaagcgatcg tcgaagctct gcacgccgag cgacaaccgg
     6661 ttgacgcccg ccgcgaaata gccggcgaat ttctcgcgct cgaaggtgcc gggattggct
     6721 tcgagcgtga tctccgcatc gggcagcaac ggcagccgcg cgcgcagtgc ggacagcagc
     6781 cgctcgatcg acgctgcaga aaacaggctc ggcgtgccgc cgccgatgaa taggctgccg
     6841 atcttgcgtc cccacaccga agggagcgcc gattcgacgt cggcgatcaa cgcggcgaca
     6901 tacgcgtcct cgggcagtgc gtcgggggcg gtcgctgccg gcgccgcctc gccgttgcgc
     6961 gagcgccact cgtgcgaatt gaaatcgcag tacgggcact tgcgcacgca ccacggcatg
     7021 tgcacgtaga gcgcaagcgg cggcagcgcc gcgaagcgtg gaccacgcag cgaatccgcg
     7081 tcgacggttc cgatggtcgc tgcgtcaacc tccatcgcgt tgcaggatct cgagccgtgc
     7141 catcaggcgc cgcatcgcct tgccgcggtg cgacagcgcg ttcttgcgcg ggagcggcaa
     7201 ctcggcgccc gtcctgcccg tgatcgcgtc ctggaaatgc gggtcgtagc cgaatccgcc
     7261 gctgccgcgc ggcgtatcga cgatggtgcc atgccatatt ccctcggcga tgaccggctg
     7321 cgggtcctcg gcgcagcgga cgagcaccag cacgcagtag taatgcgcgc ggcgatcggc
     7381 gacgtcgcgc agcgccgtaa tcagcgccgc gttgttgcgc gcatcggata cgggctcgcc
     7441 cgcatatcgc gcgctgcgca cgccgggtgc gccgccgagc gcatcgacac acacgcccga
     7501 atcatcggcc agtgcgggaa ggccgctttg cgcgctcgcg tggcgagcct tcgcgagcgc
     7561 attctcgatg aacgtcacat gcggttcgcc agcgggcgcg atgccgagtt ccgcctgcgg
     7621 gatcacctcg aggccgagcg gcgcgagcag gcgcgcgaac tcgcgcaact tgccttcgtt
     7681 gcccgatgca agcacgagac gctccatcat gcggacagcg cctcgcgctg cgcgtcgtgc
     7741 agacgtgcga tgccctgctg cgccagcgcc agcaacgcgt cgaactggtc gcgcgagaac
     7801 ggctcgcgct cggcggtgcc ttgcacctcg acgaagccgc cggcgccggt catcacgacg
     7861 ttcatgtcgg tgtcgcagcc cgaatcctcg gcgtaatcga gatcgagcag cggcgtgccg
     7921 gcgacgacgc cgacggatac cgccgcgacg cggtcgcgca gcggttcgcc ggcaagtgtg
     7981 cggcgcgcgc ggcaccaggc gattgcatcg gccagcgcaa catacgcgcc ggtgatcgac
     8041 gcgcagcgcg taccgccgtc ggcctgcagc acgtcgcagt cgatgcgtat cgtgcgctcg
     8101 ccgagcgccg cgaggtcgat gacggcgcga agcgaccgcc cgatcaggcg ctggatttcc
     8161 tgcgtccgcc ccgactgccg gccctcggcg gcctcgcgcc gcgtgcgcgt gttcgtcgcg
     8221 cgcggaagca tgccgtattc cgcggtgagc cagccctgcc ccttgccctt gagaaacagc
     8281 gggacgcctt cctcgacgct cgccgtgcac aacacgcgcg tttgcccggc ctcgatcagc
     8341 acggaccctt cggcgtgctt cgtgaaatgc cgcgcgatgc gaacggtgcg cagttcgtcg
     8401 gcgcgccggc cgcctgcgcg cgcctgcttc tgtcgggagg gttcgatggt cgccacgtcc
     8461 ggttgcaatg aatgccgatt atagagggct tgcgtgttcg ctcatgtcgc ccgccgcggc
     8521 cgcaccgacg ctcgccgcgt cgatcgacac gggccgcggc cgccaccggg ttgcatgcga
     8581 cagcgacgcg gtgtcgactt cgagcgatcc ggcgagacgc gtgaccgcgt cgctgcggcc
     8641 ggacagcgca cccgcgacgc gcagcacggt caccggcacc ggaacgagcc gcggcgcgac
     8701 gccgagcgca gcggcgatcg cgcgcacgag atccggcgtc gagatggcgg tcgcgtcggc
     8761 gacgaaatgc gtgccggcca ccggcgacgg cgcgtcgagc accgcgtcga tcgcgtccag
     8821 caggttgtcg agtgcgacga gcgaacgccg gttgtcgatc gcgccgagcg gcaggaagcg
     8881 ccgcgccgcc accgcgtcga cgagccggcg aaagttgcca cgcgcgccgc gcccgtacac
     8941 gagcggcagg cgcagcgtcg tcgcgaccat cgccgtgccg cttgcggcgc gcgcgagcgc
     9001 aagctcaccc gcgagcttgc ttcgcgcata agcgtcgtgc ggcgccgcag gatcgcccgg
     9061 ctcgaatggc cgtccgggca gcgtcgactc gccgttcacc ttcacggtac ttgcaaacac
     9121 gaaccggtgc acgccggcgg ccacggcggc cagcgcgagc cgttcggtgg cgtcgacgtt
     9181 cgcgagcctg tacgcggcct cggcatcggg cgcgaactcg ttcatgacgt gcgcgcgccc
     9241 ggcgagatga acgacggcga acgcgccgcg aagcgcctgc gtaagcggat cggcgtggac
     9301 gcgcgcgagg tcgaccgcaa cggcgtcacc gcttgcatcc ggcgcggcgt gcgcgtgtac
     9361 gaagcgccgt accggctgtc cacgtgcctg catgcgccgg caacaggccg cgccgatgaa
     9421 tccgcctgcg cccgtcacca cgatcggcgc cgcgctcaga accatttcca gccatgccgc
     9481 gcgtagaagc gcacgccgct cgcgaggaac cagcgcacat gccgcagccc cttcctcgcg
     9541 gcccggccgc cgtggtggac gacgttgacg ctcggcacga acgccgtctg cgccacgtcg
     9601 cgcaggcgca tcgtccagtc gtaatcctcg aagtacagga agaagcgcgg atcgaagcca
     9661 cccgtgcgat cgatcgcttc gcggcgcgcg agcaggaacg cgcccgacat cacgggcaca
     9721 ccgatggcct cgcgcggcgg caccgcgtcg acgatgtcgc gcatttcata acgggcgagg
     9781 cggcgcgcga acaatcgctg cacgaacccg ggcgcgaacc cgcgcagcag caggtcgaac
     9841 accgcggggt agcgcttgca gaggtactgg cgattgccgt tgccgtcggt caccgtgggc
     9901 gccagcgcgc ctacgcccgg atgtgcatcg agccagcgaa tcgcgttgac gagcgcgtcc
     9961 gcggcgagct ccgcgtcggg attgagcacg agatggtagt cggcaccggt gccgtgcagc
    10021 acgaggttgt gcgcggcgcc gaagccgatg ttcgcgtggc cgtgcaggaa caccacgtgc
    10081 acgcgcgcgt cggcaaaacg cgcgcgtccg agctcgatca ggcgctcggc gataccgcga
    10141 tcgctgctgt tgtcgatcag cgcgagatga acggtgcgca gcgcctgcgc ctgctgggca
    10201 acgtcgatcg cgatggcgag cgtatcgagc gtgcgcgtca aaagcggcag gtcgggaagg
    10261 tacgtgacga tgctgatctg cagcgacgac ggcagcacgg cgtgcgcgag gtggattttg
    10321 cgaccggcac agcatacatt gcacgccatc gatcctgcgc tggagcgccc gatgtccacc
    10381 ccgatgcccc cgccggccgc cgacacggcg acacacgccg ctgcggctgt cgaaacccgc
    10441 tacccggccg aacggctgca ggcattcgcg gtggcgctgc ttgccgccgc gggcgttcgc
    10501 gacgatgtcg cgcacgacgt cgcgtcggtg ctgctggatg gcgacctgat gggccatacg
    10561 acgcacgggc tgcagctcct gccgggctat ctcgacgcac ttgccggcgg gggaatgcga
    10621 aagacaggtg aaccggtcac gacgagttcg taccccgcga gcaggacgtg ggacggacag
    10681 cggctgccgg gaccgtggct caccttgcgc gcgctcgacg acgcgcggcg gatggcgtcg
    10741 acgcacggga ccggcacggt ggtgatccgg cgctcgcatc atatcggctg ccttgcttcg
    10801 tacgcgaagc gcgcggccga gcgcggcgtg atgacgatcg tctgctgcat cgatccggcg
    10861 acgcacagcg tcgcgccgtt cggtgcgatc acgtcggtgt tcacgcccaa tccgctcgcc
    10921 gcgggcatcc cgacgtcggg cgaacccatc ctgctcgaca tctcggcgag ctacacgacc
    10981 aacggcatga cgatgcggct gcacaggaac ggacaggaac tgccgcatcc gtgggtgcag
    11041 gacgcgcgcg gcaatccgac gcgcgatccg gccgtgctgt tcaacgagcc gaagggcacg
    11101 ctgctgccgc tcggcggtct cgacgccggc cacaagggat tcgcgcttgc gctgctgatc
    11161 gaggcgatga ccgcgggcct cgccgggcac gggcgggcgg acccggccga gggctggggt
    11221 gcgacggtgt tcgtgcaggt gctcgacccg gccgcgttcg gtggcctcga cgcgttcacc
    11281 cggcaaatgg actggctcgt gcgggcgtgc cacgaggcga cgccgcggcc ggacggaccc
    11341 ccggtgcgcc tgccgggcga gcgcgcgctg cgcctttacc gcgagcagct cgcgaacggc
    11401 gtggcgcttt atccggagat catgccggcg ctggcgcctt gggccgtgaa gctcggcgtg
    11461 ccgccgccta cggcgacgcc ttcgcctccg ccgccgcgac cgccttcttc ggattgatca
    11521 atgtgcgccg gccgtcaggg gtcttgtcgg cggtgtcgcg tatcagcgca atcacctgcg
    11581 ccggcgcgag cttcggattg acggcgagga tcttcgccgc aagattggcg acctgcggcg
    11641 aggccatcga cgttcccgat tcggcgagct tgtcgccgcc gggaatcacg ctgtcgacct
    11701 ggtagccatt cgcgtcgacg acgaccgtcg gcccgtaact cgtgaacgac gcctcgtcgc
    11761 cggccttgtc gacggcgccc acggtgatca ggttcggggc ggagatgccg gccggaatgt
    11821 cctcggtgaa cgacgcgtcc tcgttgctgt ttcccgcggc cgtcacgaac aggatatccg
    11881 gcgcgctcgc gaatgccttc gtcagcgcgt ccttctggat gtcgaaatag tcgcgcgcga
    11941 tctttttgcg ctcgtcgggc gtcttgccga tgttgcaaag ctcgagctgc tcctcctcgg
    12001 ccttgatcga gccgccccag ctcatgttga ccacgcgcac gcggtgcttc ctgaagaagt
    12061 cgacgtacgc ctgctggttt ttcgcatcct gcagcgcaag cgccttcgtc ggacacggat
    12121 cgggcagcag atgccagtcg aactcgatgc ggcccacgac gaggcgcgca tacggattac
    12181 cagcgagcgt gatgccggcc acgtgcgtgc cgtgcatcca gttgccggcg aggtcgagcg
    12241 attcgatcgc cgccttgtat tcgtccggct tcagcgtcga caggtattgc ttcacttcgc
    12301 ttgcctcggg gctgtcgatg ttcgagcgca ggtcggagaa gcccttcagg cgcgacttca
    12361 tctgcgggat ctgatccttg agcacggcgg ggatcggctg cagcgtgccg gttgcgggct
    12421 tcgtgtagcg atcgaatgca atgacggcgg gcttgccggc gtcgtcgacg acgcgtttgc
    12481 cgaacagcga cgtgtcgacg ccgctgtccc acacgccgac gttcaccggc gcatagcgct
    12541 tgcccggagg aagcacgaca tcgcgcgccg cccaaatgtc gggcttgtcg acatgatgcg
    12601 cagcaaggta gctgctgtag gtggcaacga gcgtcgcctt cagcggcagc gacgccacca
    12661 gccggtattt cgcgctgatc accaccggcg cgagatcgga gctcagcgcg ccggccttgt
    12721 cgaccgtcgg ctggatcacg ttgcgcacgt agccgagcgt cagcgcctcg ctcatgatct
    12781 cggccgacgc cttggcttcg cgcacttcgt tctgcacgac gtcgtacggc atttgcccga
    12841 ggtcggcggc gatcagccgg ccggtctcct gccgataggc gtccgatgtg cgatcgccac
    12901 tcttgcgctg tgcggcgatc atcgcgcgaa gctgcaatcc cgacatcagc ttgtcggccg
    12961 gcttgtcctg cagctcgcgc acgtgcgccg cgcgcctcaa cgcaccgtcg tcgttgccgt
    13021 cgagatagtc gagttgcgca agctcgcctt cgagctggcg cagcgtcgcc cgatcgtcga
    13081 tctcgtagcc gctcaacacc gactcggcgt cgcgacgcac ggcgctcgca aagcgcttga
    13141 acttcgcgtc gtcgccgatc acgtcctcga ctctgccctc gatcgggtac gagaagcgcg
    13201 gcaggtccgc cgccttctcg atgcgcggct tcggttgcgc gatggcactc gccgcgacga
    13261 gaaggacgag cgacacgagc agcgacacat gagagccgcg gggccgcccc aaggctcgaa
    13321 ttccgccccc gtggggggca gcgaagcggc gcagccgcga gcgtgggggg atcatgcgaa
    13381 cacgggtcgg cacgaggaag tctccgtggg gatgcgcgcg gtgcgccgcg ccgggatttg
    13441 aagtgtagcg aaagagcgcg cgacgggtcg gccttgcgcg accggacgcc ggcgggaaag
    13501 acgaaacgcc cggcgggccg acagccggtg cgaacgatca gccgcccgca agcaccgccc
    13561 gagcgggcaa gatcgcaagg agcgcgacga gcagccacgc ccagaccgcg gggtcccgga
    13621 ggagttcgta tgatcccccc acaggacgca ttgatcggcc gggctcgttg aacgttggga
    13681 atttcatgat tcggattgga gaggcgttag cggaattcgg cgcggggcga cggccttcgt
    13741 cgcagccgaa acgcgcgcga tcccggcaag atcgcgaaag acaccggcgt cggcgaggac
    13801 ggccgcacgc atcagcgcac gcacggcatc ggcctgatcg tacccgtgct cgacgatgag
    13861 gccgccaccc ggcgcgagcc acgccggcgc gcccgcgatg acgacgtgca gcgcagccag
    13921 accatcgcgg ccgccccgta gcgcggtcac cggctcgaag cgcacgtcgc cttccgcgag
    13981 gtgcggatcg gcgtcggcga cgtacggcgg gttgcttacg atcaggtcga agacgctttc
    14041 gccgccggaa agtcgttcgt accagttcga ttggagcaag cggacgttgc caagtttcat
    14101 ccgcgcggca ttgcgcgtcg ccacgacaag ggctgcggcc gaaacgtcgg tcgcgagcac
    14161 ctgcgcctgt gggcgttcac gcgcaattgc aacggcaatc gcgccggaac ccgtgccgat
    14221 gtcgagcacg cgcaccggac gatcggaagg cagcgagcgc agcgcgcact cgacgagcgt
    14281 ctcggtctcg ggacggggaa tcagcaccga tcgatcgacg gcgagcgaca ggccccagaa
    14341 ctcgcgctcg ccggtcagat aggcgacagg ctcaccgtcg cggcggcgtc gcgcgagcgt
    14401 gaagaacgcc tcggcgtcgc ggcgagcgag cggatcggtc gcgtgcgcca cgagccaggc
    14461 gcggtcggta ccgagcacgt gcgcgagcag cacctgcgcg tcgatcggcg caagacccgc
    14521 ctgcacgagc gcctgccgaa ccgttgaatg atcctccgac gcccgattgc acgccggcgt
    14581 tcggggcggc cttgccacgc tcatactcgc cattgcggcc gccacgccgc atcacggacg
    14641 cgttcgaaca cccgcaccgg ccggttgtag taacgtcccc agccggcgtc gaccatcccc
    14701 gccttcatca ggacccgctg cgatgcgagg ttgtcgggat gcgtgacgcc gaggatgcga
    14761 tagaggccga gctcgtcgaa tccgtaacgc acgagcgcgc gcgcaccttc cgttgcgagg
    14821 cccctgcccc acgcgccaaa gcgcaaccgg taaccgacct cgacttccgc cgtgccgggg
    14881 atgtacttga gcgcgaacca gccgacgaag tcgccgttgt cgcgacgcgt cgcgcgccag
    14941 gtgccgaggc cgggatagag gagatacgcg cggggaatgc gccgcagcgc gtcgtcgatc
    15001 tgctcgcgcg tcgaaaggcg gccgctgccg atgtactgca tcacgcgcgg gtcggtgtcg
    15061 agctcgtgca gctcgttgcc gtcgtcagcg gtgatctcgg aaagcgcgag gcgcttcgtg
    15121 acgcaccatg gtattgcaag gcgtcgcgga ttcgccgcgg cgctcgtccc cggggaaacg
    15181 cgagacgtgg ccggcggcag cccttctccg ttcatgcgtg cgtcgcgaga tcggcaagct
    15241 gctcggcctg gaattcctgc gccagcgcgc cgatcagctc gtcgaggtca ccctgcataa
    15301 tggcatcgat cttgtagagc gtcaggttga tgcggtgatc ggtgacgcgg ccctgcggaa
    15361 aattgtaggt gcggatgcgt tcgctgcgat cgcccgaccc gatcaggctc ttgcgatgcg
    15421 ccgcctcctt ctgctggcgc tcgcgccgct ccttgtcgac gagccgcgac gcgagcaccg
    15481 acatcgcctg cgcgcggttg cgatgctgcg accgatcgtc ctggcattcg acgacgatgc
    15541 cggtcggcac gtgcgtgatg cgcaccgccg aatcggtctt gttgatgtgc tgtccgccgg
    15601 cgcccgaagc acggaacgtg tcgatgcgca gatcggccgg gttgatcgtc acgtcggcca
    15661 ccgggtcggc ctcggggagc acggccaccg tgcaggctga tgtatggatg cggccctgcg
    15721 cctcggtttc gggaacgcgc tgcacgcgat ggccgcccga ttcgaacttg agccgcgcat
    15781 acgcgccggc gccgacgacg cgtccgatga tctcgcgata gccgccgaga tcggacgccg
    15841 attccgacac gatctcgacg cgccaccgct gccgctcggc gtagcgcgcg tacatgcgaa
    15901 acaggtcggc cgcgaacagc gccgactcgt cgccgcccgt gcccgcgcgg atttcgatga
    15961 acacgttgcg ctcgtcgctc gggtccttcg gcagcagcat cgcctgcagc tcgccttcga
    16021 gtacgacgag ccgcgcctcg gccgcgcggc gctcctcgtc cgcatagtcg cgcatctgcg
    16081 gatcgtgcgc gaggtcgacg gccgcagcga gatcgccttc cgcacgcagg aagtcgccga
    16141 agcgcgcgac gacgggatcg atttccgcgc gttcacgcgc gaggccgcga aagcggtcga
    16201 ggtcgcgtgc cgcatcctcg gccgcgagca gcgcgtcgag ctccatgagg cgcgcgctca
    16261 actggtcgag tcgggtacgt aacgagagct tcatcgtgat gcaggacgcg gtgtcggaca
    16321 gcaggaatca ggttgatgcg aggagcgcgc aaggatatcg cgcgtcgatc ggcgccgatc
    16381 accggcgccc ggcgaatacg gaggcgcagc tacccttcgg gcggcggttc cgcgcaaccg
    16441 tacacgcgcg aaaagagcgc gatcagctcg gcgcgctcgg cgtcgcccgc ggcgttgagt
    16501 gcggcgagag gcggatgcaa aaatttgctc gtcaggctcc gcgcaagcgc ctcgagaacc
    16561 tcgttcgccg gcgcgccggc cgccagcatg cgctgcgcgc ggtcgagctc gtgcttgcgc
    16621 agcgcatcgt gatgcgacgc gagcgccgcg atcgtcggca ccacgcttcg cccctgcagc
    16681 cagcgcagga aatgcgcggt ctgctcggcg atcatctgct ctgcctgcac gaccgcctcg
    16741 cggcggatcg acaggttgtc cttcacgatc tcggcgaggt cgtcgacgct gtacaggaac
    16801 acgtcgtcga gcgtcgccgc ttcggcttcc acgtcgcgcg gcacgccgag gtcgacgatc
    16861 agcaccggcg catgcttgcg ccttttcacc acgcgctcga gcgtgccctt gccgatgatc
    16921 ggcagcgagc tcgccgtgca ggtgatgatg acgtcgaact gcgcgaggcg ctcgttgagc
    16981 tcggtcagcg tgatcgcctc ggcgccgaag cgatgcgcga gctggtggcc gcgctcgagg
    17041 gtgcggttgg cgaccgtgat cgagcggggc ttccgcgcgg cgaagtgcgt ggcggcaagc
    17101 tcgatcatct cgccggcgcc gacgagcagc aggcactgat cggcgagcga gggaaagatg
    17161 cgctcggcga gcttcaccgc cgcggcggcc atcgagatcg acgcgctgcc gatgtcggtt
    17221 tgcgtgcgca cgtccttggc aactgcgaac gtgcgctgga agagccggtt cagcacgagg
    17281 ccgagcgagc ctgccgcttc ggcctggcgt accgcctgtt tcatctggcc gaggatctgc
    17341 ggctcgccga gcaccatcga atcgagaccg ctcgcgacgc gaaatgcatg cgatacggcg
    17401 cggtcgccgt tcagcgtgta aacgtacggc gacagcgacg atggtgcgag cgcgtgcgcc
    17461 tgctcgagcc agcgtgccac cggcgccggc tcgccgccat ggaagtagac ttcggtgcgg
    17521 ttgcaggtcg acaggatggc cgcttccttg acgacgcgcc ccgacgtgag ctcgcgcagg
    17581 gcgcccgaca gcgcgtcggg cgcgaacgcg acccgctcgc gcaggtcgag cggtgccgtc
    17641 gtatggttga ggccgagtgt atagagcgac atcgtgagga tggaccctcg aaggcgctcg
    17701 cgagttgcgc gcggcacgtc gccccggggt tcgagcgtat tttaccgcag cggccttgtc
    17761 gggcccgcgc caactcgttg atttgcgtca aggaaccgaa atggggccag agagcgcttg
    17821 ccacggatga acatcgaaac gacgcacgac gagaaaatcg gcccggaccg cgcgccggcg
    17881 tcgttcgcgc gccgctgctg gccgctggtg gcgctcgggc tcgtcggcat tgcgtccctg
    17941 ccgctcgtca tgctgccgac gctgcggctg ctgattcgcg acggtcatgc gcccggcatg
    18001 tcgctgccga cccttgctgc gcttacgctc atccagccga cgctattgct gatcgcggcg
    18061 gccgcgatcg gtgccgccct ggcgccgcgc ctcgcgcttt cgtctcacgt cgcgaaggtc
    18121 aacgtgcgca gcgtggtcgc ggcggaggtg cgccttgcgc tcgtgtgcgg cgtcgtgacg
    18181 ggcatcgcgg tggtcgcgct cgacatcgcg ctgtttcacg cccattcggc ggccacatcg
    18241 gcatcggcgc gcacgatcat cgacggcctg atcggcggtg cgctgtacgg cggcatcacc
    18301 gaggaaatca tgctgcgctg gggactgatg tcgctcgtcg tctgggccgg cgcacgcctc
    18361 ttcgcgcgcg gcgccgcgcg gcctgccccc ggcgtttatc gcgtggccat tgccgtcgtc
    18421 gcgctgctgt tcgcggcggg acacctgccg gcggcaatgg cgatcgcacc gctcacgacg
    18481 gcactcgtgt tccgcatcct gctgctgaat tcgcttgccg gcctcgtctt cgggtggctg
    18541 ttctggcgca ggagcctcga gtgcgcgatg ctcgcgcatg cgagcgtcca cgtcgtgttc
    18601 gcgatcgg
//
LOCUS       DASSVX010000115         2883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_220760, whole genome shotgun sequence.
ACCESSION   DASSVX010000115 DASSVX010000000
VERSION     DASSVX010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220760"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1044)
                     /gene="puuE"
                     /locus_tag="VFO53_05130"
     CDS             complement(85..1044)
                     /gene="puuE"
                     /locus_tag="VFO53_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HET8875693.1"
                     /translation="MTRIATDSAYPRDLAGYGATPPHPRWPGGARIALQFVLNYEEGA
                     ENSVLHGDAASETFLSEIIGASSFADRHLSMESMYEYGSRAGVWRVLRAFRDRAMPLT
                     IFGVAMALERNPAVAAAFVEAGHEIASHGYRWISYQNVDEAVEREHLAHAVDIISRLT
                     GAPPAGWYTGRDSPRTRRLVVEHGGFVYDADSYADDLPYWTGVDTRDGHKAHLVVPYT
                     LDTNDMRFATAQGFNSGDQFFAYLRDAFDTLYDEGDPRGLDAPKMLSIGLHCRIVGRP
                     ARTAALGRFLDYVQQHDDVWVARRIDIARHWIAHHPCGETRSA"
     gene            1131..2318
                     /locus_tag="VFO53_05135"
     CDS             1131..2318
                     /locus_tag="VFO53_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HET8875694.1"
                     /translation="MEAYAGEWLQLLIRWVHVITGIAWIGASFYFIWLDNSLRAPRGA
                     RDAPEAVGGELWAIHGGGFYHVQKYRVAPSELPAHLHWFKWEAYWTWLSGFALLVVLY
                     WWHADIYMIDRNVAAITAAQAVAISGALLVAGWVVYDQLCKRLGFAHEPVLGALLVVF
                     IAIVAWALSHVFGGRALYLQIGAMLGTMMAGNVLFVIIPAQRALVEAKTQGRAPDPVH
                     GLRGKQRSVHNNYLTLPVLLTMISNHYPMTFAHPHAWLVLMCLLLLAAYVRHFFNLRH
                     RGRTAWAIPASAALAALALAIVIAPPRPAGAIGVKFADAQSIVHERCTACHAVHPRQE
                     GIAVAPKGIVLETAAQIVANAAAIRAQAVASHAMPLGNLTGMTDDERARLGAWIDAGA
                     KTD"
     gene            complement(2337..>2883)
                     /locus_tag="VFO53_05140"
     CDS             complement(2337..>2883)
                     /locus_tag="VFO53_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET8875695.1"
                     /translation="AATKGAFRHALARHRCLVPVNGFYEWRKTASGKAPLHIGMPDAR
                     PFGLAGLYERWLSPEGEVLDTCTIVTTAACESLRDVHERMPVIVPDADYARWLDCSNP
                     DAGDLLATWSGERLRAYPVSTRVNAVRNDDAALCEPVAEGEPASISGSTKPDAPDVPG
                     EADETAADEAGEDVPDQLSLL"
BASE COUNT          433 a          957 c         1000 g          493 t
ORIGIN      
        1 ttgggacggc cccgcacgct catgggacta cctccgcgct ttgcgctccg gggccgattc
       61 gcgcttgaga ccggccccgc gcgctcatgc cgacctcgtc tctccgcatg ggtggtgcgc
      121 gatccagtgg cgcgcgatgt cgatgcgccg tgcgacccac acgtcgtcgt gctgctgcac
      181 gtagtcgagg aagcgtccga gcgcggcggt gcgcgcgggc cggccgacga tccggcaatg
      241 cagcccgatc gacagcatct tcggcgcatc gaggccgcgc gggtcgcctt cgtcgtacag
      301 cgtgtcgaat gcgtcgcgca ggtacgcgaa gaactggtcg ccgctgttga atccctgcgc
      361 cgtcgcgaag cgcatgtcgt tggtgtccag cgtgtacggg acgaccagat gcgccttgtg
      421 tccatcgcgc gtgtcgacgc cggtccagta cggcaggtcg tcggcgtaac tgtccgcgtc
      481 gtagacgaag ccgccgtgct cgacgacgag gcggcgcgtg cgcggactgt cgcgcccggt
      541 gtaccagccg gcgggcggcg cgccggtgag gcgcgagatg atgtcgacgg cgtgcgcgag
      601 atgctcgcgc tcgaccgcct cgtcgacgtt ctggtaactg atccagcgat agccgtggct
      661 cgcgatttcg tgaccggcct cgacgaacgc cgcggcgacc gcaggattgc gctcgagcgc
      721 catggcgacg ccgaagatcg tcagcggcat cgcacggtcg cgaaaggcgc gcagcacgcg
      781 ccacacgccg gcgcgcgagc cgtactcgta catcgattcc atcgacaggt gccggtcggc
      841 aaacgagctt gcaccgatga tttccgagag gaacgtttcc gacgcggcgt cgccgtgcag
      901 cacgctgttc tcggcacctt cctcgtaatt cagcacgaac tggagcgcga tgcgcgcgcc
      961 acccggccag cgcggatgcg gaggggttgc gccgtagcct gcgaggtcgc gcggataagc
     1021 gctgtcggtt gcgatgcggg tcatgcgtgc cgccgggagt atcgagtcgt cgcgagcata
     1081 caatgcgccg tccgcgccag ccaatcgatc gatcccgacg aacaagcgca atggaagcct
     1141 acgcaggcga gtggctgcag ctgctgatcc gctgggtgca tgtcatcacc ggcatcgcgt
     1201 ggatcggcgc gtcgttctac ttcatctggc tcgacaacag cctgcgtgcg ccccgcggtg
     1261 cgcgcgatgc cccggaagcg gtcggtggcg agctgtgggc gatccacggc ggcggcttct
     1321 atcacgtgca gaagtatcgt gtcgcgccga gcgagttgcc ggcgcacctg cactggttca
     1381 agtgggaagc gtattggacg tggctctcgg gctttgcgct gctcgtcgtc ctgtactggt
     1441 ggcacgccga catctacatg atcgaccgca atgtggcggc gatcaccgcg gcacaggcgg
     1501 tggcgatcag cggcgcattg ctcgtcgcgg ggtgggtcgt ctacgaccag ctatgcaagc
     1561 gtctcggctt tgcgcacgaa ccggtcctcg gagcgctgct ggtggtgttc attgcgatcg
     1621 tcgcctgggc gctgtcgcac gtgttcggcg ggcgcgcgct gtacctgcag atcggcgcga
     1681 tgctcggcac gatgatggcc ggcaacgtgc tgttcgtgat catccccgca cagcgcgcgc
     1741 tcgtcgaggc gaagacgcag ggccgcgcgc cggatccggt gcacgggctg cgcggcaagc
     1801 agcgctcggt gcacaacaat tacctgacgc tgcccgtgct gctgacgatg atcagcaatc
     1861 actacccgat gaccttcgcg catccgcatg cgtggctcgt gctgatgtgc ctgctgctgc
     1921 tggccgctta tgtgcggcat ttcttcaacc ttcgccatcg cggccgaacc gcgtgggcaa
     1981 tcccggcgtc ggcggcgctg gccgcgctcg cgctcgcaat cgtcatcgca ccaccgaggc
     2041 cggccggtgc gatcggcgtg aaattcgctg acgcgcagtc gatcgttcac gaacgttgca
     2101 cggcgtgtca tgccgtgcac ccgcgacagg agggcattgc ggttgcgccg aagggcatcg
     2161 tgctcgaaac agcggcgcag atcgtcgcca atgctgcggc aattcgcgcg caggcggtgg
     2221 cgtcgcacgc gatgcccctc ggcaacctga ccggaatgac cgacgacgag cgggcgcggc
     2281 tcggcgcgtg gatcgacgcc ggcgcaaaaa ccgactagcc cgcggtgcgc tgcgcgtcag
     2341 agcaacgaca gctggtcggg cacgtcttcg ccggcttcgt ccgcagccgt ctcgtccgct
     2401 tcgcccggca cgtccggcgc gtcgggtttc gtcgaaccgg atatcgatgc cggctcgcct
     2461 tcggcgaccg gctcgcacaa cgccgcatcg tcgttgcgca cggcattcac gcgcgtcgat
     2521 accgggtacg cgcgtaaccg ctcgccggac catgtcgcga ggagatcgcc tgcatcgggg
     2581 ttcgagcaat cgagccagcg cgcgtagtcg gcatcgggga cgatcaccgg catgcgctcg
     2641 tgcacgtcgc gcagcgattc gcatgcggcg gtcgtcacga tcgtgcaggt gtcgagcact
     2701 tcgccttccg gcgacagcca ccgctcgtaa agcccggcca gtccgaacgg acgtgcgtcg
     2761 ggcatgccga tgtgcagcgg cgccttgccc gatgccgtct tgcgccattc gtaaaacccg
     2821 ttgacgggga cgaggcagcg gtggcgcgcg agcgcgtggc gaaacgcgcc cttcgtcgcc
     2881 gcg
//
LOCUS       DASSVX010000116        11803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1653974, whole genome shotgun sequence.
ACCESSION   DASSVX010000116 DASSVX010000000
VERSION     DASSVX010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 11803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11803
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653974"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFO53_05145"
     CDS             complement(<1..225)
                     /locus_tag="VFO53_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET8875696.1"
                     /translation="MKAEADPGDQTSAADTGRYPGTAVARVEADLAIRARTKAEAAAS
                     SLMEAVVERGNLWLAYQRVVENKGAAGVDAL"
     gene            complement(681..1060)
                     /gene="ssrA"
                     /locus_tag="VFO53_05150"
     tmRNA           complement(681..1060)
                     /gene="ssrA"
                     /locus_tag="VFO53_05150"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1183..1743)
                     /locus_tag="VFO53_05155"
     CDS             complement(1183..1743)
                     /locus_tag="VFO53_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET8875697.1"
                     /translation="MQNEKRADNTMPFLTDVATLRKRARQHMDSGAVTANYGADPKVV
                     VEVLNAALATEVVCVLRYKRHYFMAQGIASESVKNEFAEHATEEQEHADRIAERIVQL
                     GGEPDLNPDTLTKRSHSEYKPGTSLVDMLREDLVAERIAVESYREIVEWLHGKDPTSK
                     RMMEDILAVEEEHAEDIQSLLAGMKD"
     gene            complement(1755..2054)
                     /locus_tag="VFO53_05160"
     CDS             complement(1755..2054)
                     /locus_tag="VFO53_05160"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875698.1"
                     /translation="MSPDVEQLTRDFRTFIADCETLFHNATTLSGSGASVARMQLSEG
                     MAAAKIKFDAMRMTAADRAARTRAATEDYVRREPMKAIAYAAGAGAVLGLLMSRR"
     gene            complement(2136..2297)
                     /locus_tag="VFO53_05165"
     CDS             complement(2136..2297)
                     /locus_tag="VFO53_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HET8875699.1"
                     /translation="MLYYAVVFFIIALIAALFGFGGIAAGAAGIAKILFVVFLIVALV
                     TFLLSLARR"
     gene            2632..3216
                     /locus_tag="VFO53_05170"
     CDS             2632..3216
                     /locus_tag="VFO53_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875700.1"
                     /translation="MKPSNAFVLAGVAVALAACNNPDPNRTTSVPSGVKNATAIVAPI
                     TTASAAPAPAERTPVPANANAVAPGTNAALAFADPNEQFAGSALPSKGGAPKNQSQAV
                     GAQQEAAQAPDTAAADAAKRGAMSGDDGARTPGTAKDSAANSPRHDALTAHEESTQMP
                     KPGQANNYSDPALEKSSGRPSDGSAAAKSQGPSN"
     gene            complement(3315..3518)
                     /locus_tag="VFO53_05175"
     CDS             complement(3315..3518)
                     /locus_tag="VFO53_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HET8875701.1"
                     /translation="MLKWSLVFFLLAVASGVLGVVDNNLTTAVMAQVFFFGNVVLFVV
                     FMLLGSIAVQAIDSPIERSRHDR"
     gene            3739..5163
                     /locus_tag="VFO53_05180"
     CDS             3739..5163
                     /locus_tag="VFO53_05180"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HET8875702.1"
                     /translation="MRRLLPLRVLLPLALGVIVSLGVLGFAEIGYRRLEFANKAMSAA
                     LEMETEVNETLALIGEAESGQRGFLLTGDPSYLKPYKAALPKIEQTFARLRELINANG
                     TAVMIEHAGQLNVLIGKKLNELESTLALNERSGREAAFQLIDTGLGQRLMEEIRTQAQ
                     SILDELRESSRRGGTRWAQDIEFGRVGMLTMTAFTIALLFVVWALARRELSAREAKRR
                     MLVEEQRRLEQEVAARTEELSELSNYLQTVREEEKSRLARDIHDELGGILVGAKMDVA
                     WAAQRCKSVLPEAAEKLNRALLILDEGVEMKRRIIEELRPTLLDNLGISSALDWQVRQ
                     ACERAGLACELNVADLELPPDVAIALYRIVQEALTNVVKYASARNVDVELLGDAEGVS
                     LIVHDDGAGLPAGVESSRLSHGIAGMRQRVRALNGTFKISSRAGSGTTIEVFMPLPQQ
                     GTEVPALEAADSPRLPAPTASAAS"
     gene            complement(5231..5818)
                     /locus_tag="VFO53_05185"
     CDS             complement(5231..5818)
                     /locus_tag="VFO53_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875703.1"
                     /translation="MSRLMISLLAAAGIAFTGAAAAADNASMSRDQYKADKDRIEAQY
                     KSDKDQCSSMKGNAEDICKAEAKGKEKVAKAELEANYKNTAKARSDARIAHADADYDV
                     AKEKCDDMSGNQKDVCVKQAKAAHTKAKADAKVARVDRDATRTAENKRADARQNARED
                     TREAQYKVAAEKCDAMSGAAKDQCVKDAKVHYGKM"
     gene            complement(5837..6076)
                     /locus_tag="VFO53_05190"
     CDS             complement(5837..6076)
                     /locus_tag="VFO53_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET8875704.1"
                     /translation="MNWDRIQGNWKQLTGKVKEQWGKLTDDEIDRIGGKRDQLAGKLQ
                     ESYGITKDEAEKQIDSFENRYGDDGAASRDANVRH"
     gene            complement(6248..7405)
                     /locus_tag="VFO53_05195"
     CDS             complement(6248..7405)
                     /locus_tag="VFO53_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET8875705.1"
                     /translation="MSKPPEPVLPRRQRATVTALIVLAAFAVCAAAKLAEGFLIPVVV
                     GMLASYSLKPMVTSLERMHIHRALGSAVVLLVLTALVAGGVFLLRDDAVSALAELPNA
                     ARKIRLAAHENARQAEGPMGHVRAAAAELNRAAAEATGAGGAAAAPPPAPPNALQRWL
                     SEQSGKALDVVVEIAVAGLLAYFLLAAGDSFRRKLVHVAGPTLAARRITVEILDEIDQ
                     QVQRYLMTMLVINTLIGIATWAILLAFGVNHAVLWGVFAAIVHIIPYAGSAVTIVATG
                     IATFVQFEELPRALAAAGLVGVAATLIGMALSAWMLGRAFRMNAVAVFVALLFFGWLW
                     GGWGLLVGVPLLAVVKTAADRIPSLERLATLLADDQRVAAPAPTGTAEAKS"
     gene            complement(7470..7625)
                     /locus_tag="VFO53_05200"
     CDS             complement(7470..7625)
                     /locus_tag="VFO53_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HET8875706.1"
                     /translation="MKRFIALALAATFALPSFALLSGCNTMEGAGKDVQAGGAKIKEE
                     ARENKKY"
     gene            complement(7874..8275)
                     /locus_tag="VFO53_05205"
     CDS             complement(7874..8275)
                     /locus_tag="VFO53_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8875707.1"
                     /translation="MDNESALRVLLVEDSPLIRERLAESIADPGRIEIIGQADSEQAA
                     IGMLSAAPWDAMVLDLQLRHGTGLGVLRALISRRPPGTKVIVLTNYAFPQYRAKSMAL
                     GADYFFDKAREYHRVREVLDDLAADRGFDVH"
     gene            complement(8406..9038)
                     /locus_tag="VFO53_05210"
     CDS             complement(8406..9038)
                     /locus_tag="VFO53_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8875708.1"
                     /translation="MIRILIADDHAIVRAGLRQFIADQVDMEVTAEAASGSETVAAVR
                     AAEFDVVLLDISMPDKNGIDTLRLLRHVKPELPVLMLSAYGEDQYAVNLLRAGASGYL
                     NKEAASTQLVGAIRTVVQGRKYVSPSLAQILADGVSGDGEKPLHAELSQREFQIFCKL
                     ASGAAVSKIADELNLSVKTVSTYRTRILEKMAMKSNADLTYYAIKNGLIE"
     gene            complement(9076..10059)
                     /locus_tag="VFO53_05215"
     CDS             complement(9076..10059)
                     /locus_tag="VFO53_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET8875709.1"
                     /translation="MAAARAASAPALGRFGRLYGSSTVMQDVYRMIEKVAPTEATVFI
                     TGESGCGKELVARTIHERSARAHGAFVAINCGAIPQNLIEAELFGHERGAFTGANRQH
                     RGCFERAEGGTLFLDEITEMAPEMQVRLLRVLEMGRFMRVGGDGEIRTNVRVLTATNR
                     DALDAVRDGRLREDLMYRLAVFPITLPPLREREGDTELLAEHFLQNLNVEGSTSKRFS
                     RAALTTIRAYHWPGNVRELKNAVHRAFIMAEDIVELDLSGLACPAVEGECLRVPVGTS
                     LAEMERQAIFATLDHCRGNKRRAAEMLGVSLKTLYNRLTAYQAEGARLASM"
     gene            10294..11733
                     /locus_tag="VFO53_05220"
     CDS             10294..11733
                     /locus_tag="VFO53_05220"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HET8875710.1"
                     /translation="MILSGGAGTRLWPLSREAAPKPFMPLPDGETLLGKTASRAIALH
                     GVDTLITITNRDYYFHTKDTYASVPAAACIYLLEPFGRNTAPAVALGALCAKARGMGD
                     RVLLVMPADHLIRDEQAFAHAVERAAQLARNGMLVTFGIAPTHPETGFGYIECGAAIE
                     PAEGALPAAHRVRRFVEKPALPAARDYVAGGHYVWNSGMFAFTADAIIDAFARHAPGV
                     LAAVRPVAQALRGLATSSMLEIDPALFAAVPDISIDYAIMEPAAAAGEVAVVRGMFDW
                     SDVGSWQAVAALTAADDDGNRGQGERVAIATRGTYVHAADRVVATVGVENLVIIDTPD
                     AVLVAHRDHLQRVKDVVGELKARGHEAYKLHRTVSRPWGTYTLLEEAPGFKIKRIEVR
                     PGAALSLQLHHRRSEHWVVVRGVARVTNGEREFDVLPNESTYIPLDTRHRLENRSADP
                     LVIIEVQCGDYLGEDDIVRFEDRYGRTPA"
BASE COUNT         2024 a         3914 c         3760 g         2105 t
ORIGIN      
        1 gagcgcatcc acgcccgctg cgcccttgtt ctcgaccacc cgttgatacg cgagccacag
       61 attgccgcgt tccactacgg cctccatcag gctcgatgca gcagcttccg ctttcgtccg
      121 cgcacggatc gccaagtccg cctcgacgcg agcaacagcc gttcccggat accgtccggt
      181 atctgctgcg ctcgtctgat caccaggatc agcttctgcc ttcatcggct tccctgagac
      241 tcccgccgcc tactcgcggc atttcatgtt cggcccttcg gctcgtgctg acgcctacta
      301 tggcctcggc tgacttctgc acctgcatcc caacacctct cgatgtcggt agcacaaggc
      361 acatgtgcag atctccccgg gtattgcgca cgtaccttca cgcttatgcc tgtcggatct
      421 acgtcgcagc gttccgtgca agtatcgggc tttgcagata ttggcctgct caccccgcta
      481 cgccgcctca tccgcttcct gtacgtcagg ccagcgcttc gccttcggct tcctccggat
      541 tcgcagtcgc ccgcgacacc cttgccgttt agctaacact tctccttgcc gagtgtgtag
      601 aggactttca cctccaagta cgtgcgccct gccgggcgca ccaagaaaaa agccgcgcga
      661 ggcggctttc aagggcggaa tggtggaggt ggcgagaatc gaactcgcgt ccggaagccc
      721 tctacagtcg ggactacatg cttagtctcg tcgtttggct ctcattcccc cgccgccggc
      781 ggacaggctg cggggaaacc agtcacctag ctttagcgct ccgtccaagt gacccggatg
      841 ggcgcgatcg ctcgaaatgt ccccgctgtg actttgcggt gtttagccgc tcatcacccg
      901 ggctgagcgc cgcccggtgc gaggtccggc gggattaagc cgccagagcg tagttttcgt
      961 cgttcgcagt taagtttgcg attgctcgag ttttacgagg acaagcgcct cggcatgccc
     1021 cgcgctgctt cgataccccc gtcgaaacca gtacaccccc ggtaacgatg tgggcgcgcc
     1081 ggaaaccccg gcgagcgctg gaaccctcaa tgatacgccg gacggtccgc tgtgtaccgg
     1141 cccgtcccga cttcaaccga gatggcgtcg agcgcgccga attcaatcct tcatgccggc
     1201 cagcagcgac tggatatcct cggcgtgctc ttcctccact gcaaggatgt cttccatcat
     1261 ccgcttcgac gtcgggtcct tgccgtgcaa ccattcgacg atctcgcggt agctctccac
     1321 ggcgatccgc tcggcaacca gatcctcgcg caacatgtcg acgagcgagg tacccggctt
     1381 gtactccgag tggctgcgtt tcgtcagcgt gtcggggttg agatcgggct cgccgccgag
     1441 ttgcactatg cgctcggcga tgcggtctgc gtgctcctgc tcctcggtgg cgtgttccgc
     1501 gaattcgttt ttcaccgact cggacgcgat gccctgcgcc atgaagtaat gccgcttgta
     1561 gcgcagcacg caaaccacct ccgttgcgag cgccgcattg agcacttcga cgaccacctt
     1621 gggatcggcg ccgtagtttg ccgtcaccgc gcctgaatcc atgtgttgcc gggctcgctt
     1681 gcgcagcgtt gccacgtcgg tcaggaacgg cattgtgttg tccgcacgct tttcgttctg
     1741 catctttgtc ttcctcaacg ccgcgacatc aggagcccga gaaccgcgcc tgccccggcg
     1801 gcataggcaa tggccttcat tggttcgcgg cgtacgtaat cctccgtcgc ggcgcgcgta
     1861 cgggcggcgc gatcggcggc ggtcatgcgc atggcgtcga acttgatctt cgcagcggcc
     1921 attccctcgg acaactgcat ccgcgcaacc gatgcgccgg agcccgaaag cgtcgtcgcg
     1981 ttgtgaaaca gcgtttcgca atcggcgatg aacgttcgga aatcacgcgt cagctgctcg
     2041 acgtcgggcg acatcggctt ggggatctgc gaaatatttc catttccgta agacgacagg
     2101 tcttgcgcac tgttttccat ggttcgacct ccggttcagc gccgcgcgag cgacaacagg
     2161 aacgtgacga gcgcgacgat caggaaaacg acgaacagaa tcttcgcgat tcccgccgcg
     2221 ccggccgcga tgccgccaaa gccgaacagt gccgcgatca acgcgatgat gaagaacacg
     2281 actgcgtaat aaagcatttt agacctcctt gggcgggttg ggcggagcgt gcgtcggcaa
     2341 cgccccaggt agcgtgacgc gcaccattcg tgccatcgcg cggaactcga aatagttccg
     2401 ggaaccaaac gcttagatcg cgagcgtcgg ccgctgcgac gaccgctttg cgcgcgagtg
     2461 ctgcaattgc tgccgcggcc gtcgggacgc tcctacatca aaatccggta cgcgccgcta
     2521 ggtggcgcgg gacgtgcgca ctatggtgga tgcacgcggc ccggacgaat tcgcccgggc
     2581 atttcgcgat gcagcgcttt tgcatcgcat tcattggtcc caggagttcg catgaaaccc
     2641 tcgaatgcat tcgtgctcgc cggcgtggcg gtggcacttg cggcttgcaa caaccccgat
     2701 ccgaaccgca cgacctcggt gccgtccggc gtcaagaatg ccaccgccat cgtcgcgccg
     2761 atcacgacgg cgagcgctgc tccggcgccc gcggagcgca cgccggtacc cgccaacgcc
     2821 aatgcggtcg cacccggtac gaacgccgcg ctcgcatttg ccgatccgaa cgagcaattc
     2881 gcggggtccg cgttaccgag caagggtggc gcaccgaaga accagtcaca ggcggtgggt
     2941 gcacaacagg aggcagcgca ggctccggac accgccgcgg ccgacgcggc gaaacgcggc
     3001 gcgatgtcgg gggatgacgg cgcgcgcacg cccggaaccg cgaaggacag cgccgcgaac
     3061 agcccgcgtc acgacgcgct caccgcgcac gaggaatcga cgcagatgcc gaagccgggc
     3121 caggccaaca actactccga tcccgcgctg gagaagagca gcgggcgtcc ctcggatggc
     3181 agcgccgccg cgaaatcgca gggaccttcg aactgaccga tgacgaacga gcagtagata
     3241 aaaaagacgc cgctatgtgg gagcggcgtc gagggcccct cgtttgggcg cgagggggag
     3301 ggagacgaac cggatcagcg gtcgtgtcgc gaccgctcga tcggcgagtc gatcgcttgc
     3361 accgcgatcg aaccaagcag catgaacacg acgaacagca cgacgttgcc gaaaaagaaa
     3421 acctgcgcca tcaccgccgt cgtgagattg ttgtcgacga cgcccagcac accgctggcg
     3481 acggcaagca ggaagaacac gagcgaccac ttcaacatga cgtcctcgcc gacaagcgac
     3541 gcgcgactgc agatgaagcg aactttaatc aagcggcatg ttcgctccta tcggagcatc
     3601 cccgatatgc gtgtaagaca aacgcttacg ccatgcgcgc aaggctttca cgtccccgct
     3661 gcgccttgag atggctgccc agccaacgta tagtgggtct tctcgttcgt ccctcgggaa
     3721 aggctccgga agtcttcgat gagacggctc ctccccctgc gtgtgctgct gcccctggcg
     3781 cttggcgtga tcgtttcgct cggcgtgctg ggctttgccg aaatcggcta ccggcgcctc
     3841 gaattcgcaa acaaggcaat gtcggcggcg ctcgaaatgg agacggaagt caacgaaacg
     3901 ctcgcgctga tcggcgaggc agagtcgggg cagcgcgggt tcctcctcac cggtgatccg
     3961 tcgtatctga agccatacaa ggcggcgctg ccgaaaatcg agcagacgtt cgcgcggctg
     4021 cgcgagctca tcaatgcgaa cggcaccgcg gtaatgatcg agcacgccgg ccagctcaac
     4081 gtgctgatcg gcaagaagct gaacgagctc gaatcgacgc tcgccctcaa cgagcgcagc
     4141 gggcgcgaag cggcgttcca gctgatcgac accggcctcg ggcagcggct gatggaggag
     4201 atccgcacgc aggcgcaaag catcctcgac gagttgcgcg aatcgtcgcg ccgtggcggc
     4261 acgcgctggg cgcaggacat cgaattcggc cgcgtcggca tgcttacgat gacggccttt
     4321 acgattgcgc tgctgttcgt ggtgtgggcg cttgcccggc gcgagctcag cgcgcgcgag
     4381 gcgaagcggc gcatgctcgt cgaggagcag cggcggctcg agcaggaggt ggcggcgcgc
     4441 accgaagagc tgtcggagct ctccaattac ctgcagaccg tgcgcgagga ggagaaatcg
     4501 cgcctcgcgc gcgacatcca cgacgagctc ggcggcattc tcgtcggcgc gaaaatggac
     4561 gtcgcatggg ccgcgcagcg ctgcaagagc gtgctgcccg aagccgccga gaagctcaat
     4621 cgcgcgctgc tgattctcga cgagggcgtc gagatgaagc ggcgcatcat cgaggaactg
     4681 cgtccgacgc tgctcgacaa tctcggcatc tcgtcggcgc tcgactggca ggtgcgccag
     4741 gcgtgcgagc gcgcgggcct tgcgtgcgag ctcaacgttg ccgacctcga gcttccgcct
     4801 gacgtggcga ttgcccttta tcgcatcgtg caggaagcgc tcaccaacgt cgtgaaatac
     4861 gcgagcgcgc gcaacgtcga cgtcgagctc ctcggcgacg cggaaggcgt ttcgctgatc
     4921 gtgcacgacg acggtgccgg cttgcccgca ggcgtcgagt cgagccggct gtcgcatggg
     4981 atcgccggca tgcgccagcg cgtgcgcgcg ctgaacggga cgttcaagat cagcagccgg
     5041 gcgggcagcg gcaccacgat cgaggtattc atgccgctgc cgcagcaagg gaccgaggtc
     5101 ccggcgctgg aggcggccga ttcgccgcgc ctgccggcgc cgaccgcatc ggctgcatca
     5161 tgaaaaacgg ccggacgtcg tccggccgct cgcctcggca ctcacgggca cccggcccgt
     5221 cgccgcgcgc ttacatcttg ccgtagtgca ccttcgcgtc cttgacgcat tgatccttcg
     5281 cggcaccgct catcgcgtcg cacttctccg ctgccacctt gtactgcgct tcgcgcgtgt
     5341 cttcgcgcgc gttttgtcgc gcgtcggcac gcttgttctc ggcggtgcgc gtcgcgtcgc
     5401 gatcaacacg cgcgaccttc gcgtcggcct tcgccttcgt gtgcgcggcc ttcgcctgct
     5461 tgacgcaaac atccttctga ttgcccgaca tgtcgtcgca cttttccttc gcgacgtcgt
     5521 agtcggcgtc tgcatgcgcg atacgcgcgt cggagcgggc ctttgccgtg ttcttgtaat
     5581 tcgcctcgag ctcggccttc gcaacctttt ccttgccctt ggcctcggcc ttgcagatgt
     5641 cctcggcgtt gcccttcatc gagctgcatt ggtccttgtc ggacttgtac tgcgcctcga
     5701 tgcggtcctt gtcggccttg tactggtcgc gcgacattga agcgttgtcc gcggccgccg
     5761 cggcgccggt gaacgcgatg ccggccgcgg ccagcaagct gatcatcaat cgtgacatgg
     5821 taggtatccc cttcggtcag tgacggacgt tggcgtcgcg cgaggcggcg ccgtcatcgc
     5881 cgtagcgatt ttcgaacgaa tcgatctgtt tctcggcttc gtccttcgtg atgccatacg
     5941 actcctgcag cttcccggcg agctggtcac gcttgccgcc gatgcgatcg atctcgtcgt
     6001 cggtgagctt tccccattgc tccttcacct tcccggtgag ttgcttccag ttgccttgaa
     6061 tgcgatccca gttcatgcgt tgctccttcg gcgtgcggac caatgcgggc ccgatcgtgc
     6121 cttggatgct gcaacgggcg tgccagcaga attcccttcg ctgtcaagca cttggcaatc
     6181 agcggccggg cagggttgaa aatcgcgttg ccgccggatg gaacttcggc gcgcactgta
     6241 ggacatctca ggatttggct tcggcggtgc ctgtcggcgc gggggctgcg acgcgctgat
     6301 cgtcggcgag caatgtggcg aggcgttcga gcgaagggat ccgatcggct gccgttttca
     6361 ccacggccag aagcggcacg ccaaccagca ggccccagcc gccccagagc cagccgaaga
     6421 acaacagcgc gacgaatacc gcaaccgcgt tcatgcggaa tgcgcgaccg agcatccacg
     6481 cgctcaacgc catgccgatc agcgtcgccg caacgccgac gagcccggca gcggccagcg
     6541 cacgcggcag ctcttcgaac tgcacgaacg tggcgatgcc ggtcgcgaca atggtcacgg
     6601 cggaaccggc atacgggatg atgtgcacga tcgcggcgaa cacgccccac agcaccgcat
     6661 ggttgacgcc gaacgcgagc aggatcgccc acgtggcgat gccgatcagc gtgttgatga
     6721 cgagcatcgt catcaggtag cgctgcacct gctggtcgat ctcgtcgagg atctcgaccg
     6781 tgatgcgccg cgcagcgagc gtgggacccg cgacgtggac gagcttgcgg cgaaaggaat
     6841 cgccggccgc gagcaggaaa taggcaagaa gccccgcgac ggcgatttcg acgacgacgt
     6901 cgagcgcctt acccgactgc tccgaaagcc agcgctgcaa agcgttgggc ggcgctgggg
     6961 gcggggccgc ggcggcaccg cccgcaccgg tcgcttccgc cgcggcgcga ttgagctccg
     7021 ccgcggccgc gcgcacatga cccatcggcc cctcggcctg gcgtgcgttt tcatgcgcgg
     7081 cgaggcgaat cttgcgtgcg gcgttcggaa gctcggcaag tgcggacacc gcgtcgtcgc
     7141 gcagcaggaa gaccccgccc gcgacgagcg ccgtcagcac gagcaacacg accgccgacc
     7201 cgagcgcgcg gtgaatgtgc atgcgctcga gcgacgtaac catcggcttc agcgaatagc
     7261 tcgccagcat gccgacgacg acgggaatca ggaacccttc agcgagcttg gcggccgcgc
     7321 acacagcgaa cgcagccagc acgatgagtg cggtgacggt cgcccgctgc cgccgcggca
     7381 ggaccggttc cggaggcttg ctcatatgca aacggcgcct ggagccgggt cgtcgactcc
     7441 agacgccgtc ggttcaggcg cttcccggat cagtacttct tgttctcgcg cgcctcttcc
     7501 ttgatctttg cgcctcccgc ctggacgtcc ttgccggcac cttccatggt gttgcagccg
     7561 gaaagcaatg caaacgacgg aagcgcgaat gtcgccgcta gcgcgagtgc gatgaagcgt
     7621 ttcatgttgt cccctttcat ggagtggtga tgatgcacga gccgccgttg cggtcgagcc
     7681 gcgctttggc tatgcagccg ccgtgccacg gcgaaaagcg ttatcgttca tgaggtttcg
     7741 caggccccgc ctaccggcgg ttgcaacgtc gcattcgatc accggcaatt cttacacccg
     7801 tcgtcagcgc gctccgacgg catgccggga gtacgcgcgc cggagcagcg cggcgcgacg
     7861 ccgaaggcgt gaatcagtgc acgtcgaaac cgcggtcggc ggcgagatcg tccagcacct
     7921 cgcgcacgcg gtgatattcg cgcgccttgt cgaaaaaata gtcggcgccg agcgccatgc
     7981 tcttcgcgcg atattgcggg aacgcatagt tcgtgagcac gattaccttc gtgcccggcg
     8041 gacgtcgcga gatcagcgcg cgcagcacgc cgagccccgt tccgtgccgc aactgaaggt
     8101 cgagcaccat cgcgtcccat ggcgccgcgc tcagcatgcc gatcgcggcc tgctcgctgt
     8161 cggcctggcc gatgatctcg atgcgaccgg gatcggcgat cgactccgca aggcgctcgc
     8221 ggattagcgg cgaatcctcg acgagcagca cacgaagagc cgattcgtta tccactcccg
     8281 tccgatcgag cgataccccg ggcagcgccg gatcttcatc agtggaaggt acccctgtaa
     8341 gtcgcatttg cctcgcgtcc accggtcaca cgctcagtgt cgcaagcgcc agcacccgtg
     8401 gttcctcatt caatgagtcc gttcttgatg gcgtagtacg tgaggtcggc gttcgacttc
     8461 atggccatct tttcgagaat gcgcgtccgg tacgtgctca cggtcttcac cgagagattg
     8521 agctcgtcgg cgatcttcga gaccgccgcg ccggacgcga gcttgcagaa gatctggaac
     8581 tcgcgctgcg acaattcggc gtgcagcggc ttttcgccgt cgcccgagac gccgtcggcg
     8641 agaatttgcg caagcgacgg acttacgtac ttgcgtccct gcacgacggt gcgtatggcg
     8701 ccgacgagct gcgtcgacgc cgcttccttg ttgagatagc ccgacgcgcc ggcgcgcagc
     8761 agattgaccg cgtactggtc ctcgccgtac gccgacagca tgagcaccgg cagctcgggc
     8821 ttcacgtgcc gcaggagccg caaggtgtcg atgccgttct tgtcgggcat cgagatgtcg
     8881 agcagcacga cgtcgaactc ggccgcgcgc acggcggcca cggtctcgga gccgctggcg
     8941 gcttcggccg tgacctccat gtcgacctgg tcggcgatga actgtcgcag cccggcccgg
     9001 acgatggcgt gatcgtccgc gatcaagatt cggatcatcg agtgcccgta atcacgcgaa
     9061 tcgcgcgatt ctagcctaca tggacgcgag tcgagcacct tctgcctggt acgcagtgag
     9121 ccggttgtag agcgttttca ggctgacgcc cagcatctcg gcggcgcgcc gcttgtttcc
     9181 ccggcagtgg tcgagcgtgg cgaagatcgc ctggcgttcc atctcggcga gcgacgtgcc
     9241 gacaggcacg cgcaggcact cgccttcgac cgctgggcac gcgagtccgg acagatcgag
     9301 ctcgacgata tcctccgcca tgatgaatgc acgatgcacg gcgttcttga gctcgcgcac
     9361 gttgccgggc cagtgatacg cacggatcgt cgtcagcgcc gcgcgcgaga agcgcttgga
     9421 tgtgctgccc tcgacattga gattctgcag gaagtgctcg gcgagaagct cggtgtcgcc
     9481 ctcgcgctcg cgcagcggtg gcagcgtgat cgggaacacc gcgaggcggt acatgagatc
     9541 ttcgcgcagg cgcccgtcgc gcacggcatc gagcgcatcg cggttggtgg ccgtcagaac
     9601 gcgcacgttc gtgcggatct cgccgtcgcc gccgacgcgc atgaagcgac ccatctcgag
     9661 gacgcgcagc aggcggacct gcatttcagg cgccatctcg gtgatctcgt cgaggaacag
     9721 cgtgccaccc tcggcgcgct cgaaacagcc gcggtgctgc cggttggcgc cggtgaacgc
     9781 gccgcgctcg tgcccgaaca gctcggcttc gatcaggttc tgcggaatgg cgccgcagtt
     9841 gatcgcgacg aacgcgccat gcgcacgcgc gctgcgctcg tggatcgtgc gtgcaaccag
     9901 ctccttgccg cagcccgact cgcccgtgat gaagacggtg gcctcggtgg gcgcgacctt
     9961 ctcgatcatc cggtatacat cctgcatcac ggtcgaactg ccgtacaggc gtccgaaacg
    10021 tccaagcgcc ggtgcggacg cggcacgcgc agcggccatg aacggcagcg cgacgacgat
    10081 gtcgttggtc ggttcagcgg tgagcgtttc atgaaggggc atcgaattcc tctaggtgat
    10141 ttgtcgcaac agacgcatcc tagggaaaac tttgcatcga ccgtatcggc gcctcccgga
    10201 acctattgtc caagtttgtc ctacagccgt tcatgcgcta gagtgttgcg tcgacttccg
    10261 gtaaaaatca tgcgcagcac atcgattctt ccgatgattc tttccggcgg cgccgggacg
    10321 cgattgtggc ccctgtcgcg cgaagcggca ccgaagccgt tcatgccgct gcccgacggc
    10381 gaaacgctgc tcggcaagac tgccagccgc gcgatcgcgc tgcacggcgt cgacacgctc
    10441 atcaccatca ccaatcgcga ttactacttc cataccaagg acacgtacgc ctcggtgccg
    10501 gccgcggcct gcatttacct gctcgagccg ttcggccgca acacggcgcc cgccgtcgca
    10561 ctcggcgcgc tgtgcgcgaa ggcgagaggc atgggcgatc gcgtgctgct tgtcatgccg
    10621 gccgaccacc tgatccgtga cgagcaggcg ttcgcgcatg ccgttgaacg cgcggcgcag
    10681 ctcgcccgga acggaatgct cgtcacgttc ggcattgcac cgacgcaccc cgagaccggc
    10741 ttcggctaca tcgagtgcgg tgcggcgatc gagcccgccg aaggcgcatt gccggccgcg
    10801 catcgggtgc gccgtttcgt cgagaagccc gcgctgcccg cggcgcgcga ttatgttgca
    10861 ggcggccact acgtatggaa ttccggcatg ttcgcgttca cggccgacgc gatcatcgac
    10921 gcgttcgcgc gccacgcgcc cggcgtgctc gccgccgtgc ggccggttgc acaagcgctg
    10981 cgcggtctcg ccacgtcgtc gatgctcgag atcgatcctg cgctgttcgc ggcggtcccc
    11041 gacatttcga tcgactacgc gatcatggaa ccggcggctg cagccggcga agtggcggtc
    11101 gtgcgcggaa tgttcgactg gagcgacgtc gggtcctggc aggcggttgc ggcactcacc
    11161 gccgcggacg acgacggcaa ccgcggccag ggcgagcgcg ttgcaatcgc cacgcgcggc
    11221 acgtacgtgc acgccgccga ccgcgtcgtg gccaccgtcg gcgtcgagaa tctcgtgatc
    11281 atcgatacgc ccgacgccgt gctcgtcgcg catcgcgatc acctgcagcg ggtcaaggat
    11341 gtcgtcggcg aattgaaggc gcgcgggcac gaggcgtaca agctgcaccg caccgtcagc
    11401 cggccatggg gcacgtacac gctgctcgag gaagcgccgg gcttcaagat caagcgcatc
    11461 gaggtgcgcc ccggcgcagc gctctcgctg cagcttcatc accggcgcag cgagcactgg
    11521 gtggtcgtgc gcggtgtcgc gcgcgtcacg aacggcgaac gcgagttcga cgtgctgccg
    11581 aacgagtcca cgtacatccc gctcgacacg cggcatcgcc tcgaaaaccg cagcgccgat
    11641 ccgctggtca tcatcgaggt gcagtgcggc gactatctcg gcgaagacga catcgtgcgc
    11701 ttcgaggacc ggtacggccg cacgccggcg tgagccgtcg gcgtaattgg cggcgtaatt
    11761 ggggtcacgt aattggggtc agagacgcat ttccggacgc gat
//
LOCUS       DASSVX010000117         7331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1504026, whole genome shotgun sequence.
ACCESSION   DASSVX010000117 DASSVX010000000
VERSION     DASSVX010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504026"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..1003
                     /locus_tag="VFO53_05225"
     CDS             8..1003
                     /locus_tag="VFO53_05225"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HET8875711.1"
                     /translation="MPTIADAPPYAGLTPDCVLDALDSVGMRGDGRLLALNSYENRVY
                     QAWRDDAPPVVVKFYRPGRWSDAQIHEEHVLVAELAQADVPAVAPMVLGETTLHAFGG
                     YRFAVFARHGGRAPELDRRDTLVQLGRYIARIHTVGAARRFEQRPALTIEDFGDRSRA
                     YLLDHQFLPPDLLAAWQGIVELALAGVRGAYSRVGVASLRLHGDCHGGNVLWTDDGPH
                     FVDFDDARTGPAVQDLWMLLSGDRPAMEAQLGALLEGYESFRDFDPRELALVEPLRTL
                     RLLHYSAWIARRWDDPAFPAAFPWFNTQRYWQDRILELREQVALMDEPPLRAPRR"
     gene            1138..3777
                     /gene="ligD"
                     /locus_tag="VFO53_05230"
     CDS             1138..3777
                     /gene="ligD"
                     /locus_tag="VFO53_05230"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HET8875712.1"
                     /translation="MGLELYRRKRNFKTTPEPAGRVRRARAREPVFVIQKHAASHLHY
                     DFRLELNGVLLSWAVPKGPSLDPHDKRLAMHVEDHPIEYGEFEGVIPPKQYGSGTVLL
                     WDRGTWLPQGDAEAAYRQGKLKFELHGEKLRGGWMLVKSHGGKYGGDKSWLLIKENDD
                     YARSGADAHVVDTEPDSVASGRSIEAIAADPDRVWHSNKSVAENVRTGAVRRRKPALS
                     PAKVEGARKVAQPESMDAELATLVDRAPPGDEWVHEIKFDGYRMLSRIADGRCRIVSR
                     NGKDWTTSFPAIVDALERLPVKSAWIDGEIVVMDAKGRTSFQALQNVLSEGTAAKLVY
                     CVFDLPYLDGYDLRDATLDARKAMLGKLAGEGSVIRYSDHVRGNGASFFAEACKLGLE
                     GIVSKRADSRYRGSRGRDWQKVKCGLRQEFVIGGYTEPQGARTGFGALLLGVHERGEL
                     RYCGKVGTGFNDATLTTLAKRLSKLATDKPPFANPPTGAEGRRAHWVKPELVGEVSFT
                     EWTRDGTLRHPSFQGVREDKPARDIVRETPAGNAADSGAQGDTAEPKPAATRPARKPS
                     ARRRAPGEIADAADAIEGVAISNASKLLYPEAGITKLDVARYYAAVGEKLVAQLRDRP
                     LTLVRCPNGWDKPCFYQKHANESVSQYIDRIDIRDSGGVKPYMMANSVSAVVALLQMG
                     VLELHPWGSRAPKLGYPDRLIFDFDPDEALGFDKLVDAVTVLRKLLDTLGLEAFLKTT
                     GGKGLHVVVPLEPTRPWDEAKEFCRGIAELLVRTFPDRFIAKATKSRRSGLIFVDYLR
                     NAEGATAVAAYSVRAKANAPVAMPIDWAELDKDVRFDYFNARNVPARLRKRKRDPWDR
                     LPRVRQALTDAMLAQVRSGATRR"
     gene            3779..4732
                     /locus_tag="VFO53_05235"
     CDS             3779..4732
                     /locus_tag="VFO53_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET8875713.1"
                     /translation="MARAIWKGTISFGLVNVPVELYPASQSHTLSFSMLDKRDFSPVG
                     YKRYNKSTGAEVPWNDIVKGYEYEKDQYVVMSDEDFRRANVQQAKSVEIATFVDAKSI
                     PPQYFETPYYLVPGERGQKVYALLRETMRATGKVGVGQVVIRTSHHLAAVVPVGEALM
                     MLTLRYANELRKEKEFDFPPMSLKTAGVTAKEMELAQKLIADMSGEWKPEEFEDTYNE
                     DLLQRIEEKVKHGETKVLTKPEAEAGEGRSAQVIDLMELLKRSIDKTGARAAPDRGAK
                     RAAANADEEAAAPRAATRARKTSAAHKATAERKPAARRKRA"
     gene            4760..5530
                     /locus_tag="VFO53_05240"
     CDS             4760..5530
                     /locus_tag="VFO53_05240"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET8875714.1"
                     /translation="MAEQKWPQVLWIVRHGQSAGNVAREAAEAAAHPLIDITTRDVDT
                     PLSVLGERQSLALGRWFGAMPVHRRPTVLLSSPYLRAVETSRLVLEAAGIDATALATQ
                     RDERLREKEFGVLDRLTKVGIRHKYPELSEQRAHVGKFYFRPPGGESWCDVLLRLRSV
                     LDTMTREHRRQRVLIVAHQVIVNCLRYLLERMSEEQILAIDTAADVPNCSVTSYEFEP
                     TAGRAGKLLPRLINFVAPLEEAGAPITAAPDVPGAPKS"
     gene            5667..6449
                     /locus_tag="VFO53_05245"
     CDS             5667..6449
                     /locus_tag="VFO53_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875715.1"
                     /translation="MRTLRLLLVALALGSFAWTARASSGTNFSDQWWNPDESGWGASI
                     LQQADVLFVDLFVYGADGRPTWFTAAAYFQPHSGRVLFSGDLYVTSGPWFGAFFNPNS
                     VFSRKVGTLQFDASSTDFATLTYSVDGVVVNKQIERQLWAYEDFTGSYFGGLVYDQSG
                     CAVASDNGHVEEFGAFQIDHPATNTFTLSLQSNFGNCTITGNYSQLGHMGTVDANYAC
                     SYGINGTLTLYELERTSSGMTGSFIGQNNACQVSGTLGGVAR"
     gene            7119..>7331
                     /locus_tag="VFO53_05250"
     CDS             7119..>7331
                     /locus_tag="VFO53_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875716.1"
                     /translation="MCGLPSDVVGEIHQAVEGSAGRRFRSRANKVGDAAIPAGQGSPL
                     LGHADIRITSRHDAHLRCRTLANAVNA"
BASE COUNT         1321 a         2396 c         2448 g         1166 t
ORIGIN      
        1 gacctgaatg ccaacaatag cagacgcgcc gccttatgcc gggctgacac ccgattgcgt
       61 gctcgacgcg ctcgacagcg tcggcatgcg cggtgacgga cgcctgcttg cgctcaacag
      121 ctacgagaat cgcgtctacc aggcgtggcg cgacgatgcg ccgcccgttg tcgtcaagtt
      181 ctaccggccc ggccgctggt ccgatgcgca gattcacgaa gagcatgtcc tcgtcgccga
      241 gcttgcgcag gccgacgtcc ccgcggtcgc gccgatggtg ctcggcgaaa ccacgctgca
      301 tgcgttcggc ggttaccggt tcgccgtgtt cgcgcgccac ggcggtcgcg ccccggagct
      361 cgaccgccgc gacacgctcg tgcaactcgg gcgctacatc gcgcgcatcc acacggtggg
      421 tgccgcccgc cggttcgagc agcgccccgc gctgacgatc gaagacttcg gcgaccgatc
      481 gcgtgcctac ctcctcgacc accagttcct gccgccagac ctgctcgcgg cgtggcaggg
      541 catcgtcgag ctcgcgctcg ccggcgtgcg cggcgcgtat tcgcgcgtcg gcgtggcgtc
      601 gctgcggctg catggcgact gtcacggcgg caacgtgctg tggaccgacg acggaccgca
      661 tttcgtcgac ttcgacgatg cgcgcaccgg accggcggtg caggacctgt ggatgctgct
      721 ctccggcgat cgccccgcga tggaggcgca gctcggcgcg ctcctcgaag gctacgaatc
      781 gtttcgcgac ttcgacccgc gcgagctggc gctcgtcgag ccgctgcgca cgctgcggtt
      841 gctgcattac agcgcgtgga tcgcgaggcg ctgggacgac ccggcgtttc ctgccgcgtt
      901 tccatggttc aacacgcagc gctattggca ggaccgcatc ctcgagctgc gcgagcaggt
      961 cgcgttgatg gatgaaccgc cgctccgcgc accgaggcgc tgactacgcg cgaccggcgc
     1021 acgcccgtat ccgaaacgcg tgtcggagtc cgcctacgcc gttttcagcg gccacgtgct
     1081 gtgcgctcgg cgcgccggcg gcagaatgtg aagcctgcca cgatcgcccg gacgacgatg
     1141 gggctcgaac tctatcgcag gaagcgcaac ttcaagacga cgcccgagcc cgccggccgc
     1201 gtgcgtcgag cccgcgcacg cgagccggtg ttcgtcatcc agaagcatgc ggcaagccat
     1261 ctgcattacg acttccgcct cgagctcaat ggcgttctgc tgagctgggc cgttcccaag
     1321 gggccgagcc tcgacccgca cgacaagcgc ctcgcgatgc acgtcgagga ccacccgatc
     1381 gagtacggcg agttcgaagg cgtcattccg ccgaagcagt acgggtccgg caccgtgctg
     1441 ctgtgggacc gcggtacctg gctgccgcaa ggcgacgccg aggccgccta tcgccagggc
     1501 aagctcaagt tcgagctgca tggcgaaaag ctgcggggcg gctggatgct cgtcaaaagc
     1561 cacggcggca agtacggcgg cgacaagagc tggctgctca tcaaggagaa cgacgactac
     1621 gcgcgatcgg gtgccgatgc gcatgtcgtc gacaccgagc ccgacagcgt cgcgtcgggg
     1681 cgtagcatcg aagcgatcgc ggccgatccc gatcgcgtat ggcactcgaa caagtcggtg
     1741 gccgagaacg tgcgcaccgg cgccgtgcgg cggcgcaagc cggcattgtc gccggcgaag
     1801 gtcgagggcg cgcgcaaggt ggcgcaaccc gaatcgatgg atgccgagct cgccacgctg
     1861 gtcgatcgcg cgccaccggg cgatgaatgg gtgcacgaga tcaagttcga cgggtaccgg
     1921 atgctgtcgc gcatcgccga cggacgatgc cggatcgtct cgcgcaacgg caaggactgg
     1981 acaacgtcgt tcccggcgat cgtcgacgcg ctcgaacggc tgcccgtgaa aagcgcgtgg
     2041 atcgacggcg agatcgtcgt gatggacgcc aagggccgca ccagtttcca ggcgctgcag
     2101 aacgtgcttt cggaaggtac ggctgcaaag ctcgtctact gcgtcttcga cctgccctac
     2161 ctcgacggct acgaccttcg cgacgcaacg ctcgatgcgc gcaaggcgat gctcgggaag
     2221 ctcgccggtg aaggatcggt gattcgctac agcgaccacg tccggggcaa cggcgcctcc
     2281 tttttcgccg aagcgtgcaa gctcgggctc gaaggcatcg tgtcgaagcg cgccgattcg
     2341 cgctaccggg gaagccgcgg gcgcgactgg cagaaggtga aatgcggcct gcgccaggaa
     2401 ttcgtcatcg gcggctacac cgagccgcag ggcgcgcgca cggggttcgg cgcattgctc
     2461 ctcggcgtgc acgaacgcgg cgagctgcgc tattgcggca aggtcggcac aggcttcaac
     2521 gacgcaacgc tcacaacgct cgcgaagcgt ctgtcaaagc tcgcgaccga caagccgccg
     2581 ttcgccaatc cgccgaccgg cgccgaagga cgccgcgcgc attgggtcaa gcccgagctc
     2641 gtcggcgaag tttcgttcac cgaatggacg cgcgacggca cgcttcgcca cccgtcattt
     2701 cagggcgtgc gcgaggacaa gccggcgcgc gacatcgtgc gcgaaacgcc tgccggcaac
     2761 gcggcggaca gtggagcgca aggcgatacc gcagagccga agcctgccgc gacgcgaccc
     2821 gcgcgaaagc cgagcgccag gcggcgcgcg ccgggcgaga tcgcggacgc cgcggacgcg
     2881 atcgaaggtg tggcgatcag caacgcgagc aagcttctgt atccggaggc ggggatcacg
     2941 aagctcgacg tcgcccgcta ctacgccgcg gtcggtgaaa agctcgtggc gcagctgcgc
     3001 gatcgcccgc tcacgctcgt gcgctgcccc aacggctggg acaagccctg cttctaccag
     3061 aagcatgcaa acgaatcggt gtcgcaatac atcgaccgca tcgacattcg cgacagcggc
     3121 ggcgtgaagc cgtacatgat ggcgaacagc gtcagtgccg tcgtcgcgct gttgcagatg
     3181 ggcgtgctcg agctgcatcc gtgggggtcg cgcgcgccga agctcggtta tcccgaccgc
     3241 ctgatcttcg acttcgaccc cgatgaagcg ctcggcttcg acaagctcgt cgacgcggtc
     3301 accgtgctgc gcaagctcct cgacacgctc gggctcgaag cgttcctgaa gacgaccggc
     3361 ggcaagggcc tgcacgtcgt cgttccgctc gagccgacac ggccctggga cgaggcgaag
     3421 gagttctgcc gcggcattgc cgaattactg gtgcgcacgt ttcccgaccg cttcatcgcg
     3481 aaagccacga agtcgcgacg cagcgggctc atcttcgtcg actacctgcg caacgccgaa
     3541 ggcgccaccg cggttgccgc ctattcggtg cgcgcgaagg cgaacgcgcc ggtggcgatg
     3601 ccgatcgact gggcggagct cgacaaggat gtgcgcttcg attacttcaa cgcacgaaac
     3661 gtccccgcgc ggctgcgcaa gcgcaagcgc gatccctggg accggctgcc gcgcgtgcgg
     3721 caagcgctga ccgacgcgat gcttgcgcaa gtgcgctccg gcgcgacgag gagatgacat
     3781 ggcgagggca atctggaaag gcaccatcag cttcgggctc gtcaacgttc cggtggagct
     3841 ctacccggca tcgcaatcgc acaccctttc gttctcgatg ctcgacaagc gcgacttcag
     3901 cccggtcggc tacaagcgct acaacaagag caccggggcg gaggtgccgt ggaacgacat
     3961 cgtcaagggc tacgaatacg aaaaggacca gtacgtcgtg atgagcgacg aggattttcg
     4021 ccgcgcgaac gtgcagcagg cgaaatccgt cgagattgcc acgttcgtcg acgcgaagag
     4081 cattccgccc cagtatttcg agacgccgta ctacctcgta cccggcgaac gcgggcagaa
     4141 ggtctatgcg ctgctgcgtg aaacgatgcg cgcgacaggc aaggtcggcg tcgggcaggt
     4201 cgtgatccgc acgtcgcacc atcttgccgc cgtcgtcccc gtcggtgagg ctctgatgat
     4261 gctcacgctt cggtatgcga acgagctgcg caaggagaag gagttcgatt tcccgcccat
     4321 gtcgctcaag accgcgggtg tgacggcaaa ggaaatggag ctcgcgcaga agctgatcgc
     4381 ggacatgtcg ggcgagtgga aaccggaaga gttcgaggac acctacaacg aagacctgct
     4441 gcagcgcatc gaggagaagg tgaagcacgg cgagacgaag gtgctgacga agcccgaggc
     4501 cgaagccggc gagggacgct cggcgcaggt gatcgacctg atggagcttc tcaagcgcag
     4561 catcgacaag accggcgcac gtgccgcccc cgatcgtggc gccaaacggg ccgccgcgaa
     4621 cgccgacgag gaggccgcgg cgccgcgggc cgccacgcgt gcccgcaaaa cgtccgccgc
     4681 acacaaggcg acggcggaga ggaagccggc ggcgcgccgc aagcgtgcct gaagcttggc
     4741 gtgggccgca tcaccgacga tggccgagca gaagtggccg caggtcctgt ggatcgtgcg
     4801 gcacggacaa agtgccggaa acgtcgcgcg cgaagccgcc gaggccgcag cgcacccgct
     4861 gatcgacatc acgacgcgtg acgtcgatac tccgctgtcg gtgctcggcg agcggcaatc
     4921 gctggcgctc ggacgctggt tcggcgcaat gccggtgcat cgcaggccaa ccgttctgct
     4981 gtcctcaccg tatctgcgcg cggtggagac ctcgcgactc gtgctcgagg cggcgggcat
     5041 cgatgcgacc gcgctcgcga cgcaacgcga cgagcgattg cgcgaaaagg aattcggcgt
     5101 tctcgaccgc ttgaccaagg tcggaatccg ccacaaatat cccgagctgt ccgagcagcg
     5161 cgcgcacgtc ggcaaattct atttccgccc gcccggcggc gagagctggt gcgacgtgct
     5221 gctgcgcctg cgcagcgtgc tcgacacgat gacgcgcgag caccgccgcc agcgcgtgct
     5281 gatcgtcgcc caccaggtga tcgtcaactg cctgcgctac ctgctcgagc ggatgagcga
     5341 agagcagatc ctcgcgatcg atactgcggc cgacgtgccg aattgctcgg tcacgtcata
     5401 cgagttcgag ccgaccgcgg gtcgcgccgg caaactgctg ccgcgcctca tcaattttgt
     5461 cgcaccgctc gaggaggccg gggcaccgat aaccgcagcg cccgatgtcc ccggcgcacc
     5521 gaagtcctag cttgcggatg caccgtctcg agggagtagg acgaacaccg acgacgatgc
     5581 ccgcaaattg ctgatggcca ccgggcgcgg tgcgccgcaa agatggcttc cgttcgaccc
     5641 tgatcgcgtt ccctgccgga gacccgatgc gcacccttcg cctgcttctc gttgccctcg
     5701 cccttggttc gtttgcatgg accgcgcgag catcctccgg caccaacttc agcgaccaat
     5761 ggtggaaccc cgatgaatcg ggctggggag cgtcgatcct gcagcaggcc gacgttctgt
     5821 tcgtcgacct cttcgtctac ggggccgacg ggcggccgac atggttcacc gccgccgcat
     5881 atttccagcc gcactcgggc cgcgtgctct tttccggcga tctctacgtg acgagcggtc
     5941 cgtggttcgg tgcattcttc aatccgaact cggtgttctc gcgcaaggtg ggcacgttgc
     6001 agttcgatgc gagcagcacc gatttcgcga cgctcaccta tagcgtcgat ggtgtggtcg
     6061 tcaacaagca gatcgagcgg cagctatggg cctacgagga cttcaccggc agctacttcg
     6121 gtggcctcgt ctacgatcaa agcggatgcg cggtggcaag cgacaatggc cacgtcgagg
     6181 aattcggcgc gttccagatc gatcatccgg cgaccaacac gttcacgctg tcgctgcagt
     6241 cgaacttcgg caactgcacg atcaccggca actacagcca gctcgggcac atggggacgg
     6301 tcgacgcgaa ctacgcctgc tcgtacggga tcaacggcac gctgacgctc tacgagctcg
     6361 aacggacgtc gtccggcatg accggcagct tcatcggcca gaacaacgcg tgccaggtgt
     6421 cgggaacgct cggaggcgtc gcgcgctgac gatgcgtgca gtgcgtccgt gcgcacgtgc
     6481 cgatgtcact ctgcccgttt tatcgccgcg accgggcctt tgtcgaacgt cgcaagcaat
     6541 ggcgcccgag ccagacgcca ccgccggaag tgttggctgc gtggtgcgcg catctttatt
     6601 cgcccgtgag caagttcgtc gcgacgagga tcaccggtgg cgcggccaag cttcaatgcg
     6661 aaggtgatct cgcgaagtgc caggtgcgcg aggcggtgcg tcccaaaatc tgagttgggc
     6721 cggcctaatc aaccgagtac ttgtcgacgc agatgtagcc gatgccacaa agaccatcgc
     6781 gtgtcctgac ataggtgaat tggggcgttt gccagaactg gcaccggggg acggcgggac
     6841 cgtagaaggt atcgccccag ctcccgcgag gtcccgagag gatgctcagt tctccagtag
     6901 acttgagttc ggccttcaag tcgttgcagg acaggctctg cgggttaacc ggcgcaggag
     6961 tttgttgttc gttcccggga accggaccac ccgcgggtgg ggcttgcgca ccctgaccgt
     7021 agcttgcaga cgttgacagg aaggcagccg cagccgcgca cgttgcaaca aacctgcgtg
     7081 gggcaggcaa tgcggatgat tttctggtcg gtggttgcat gtgcggtctg ccgagtgacg
     7141 tcgtagggga aattcatcag gcggtggaag gctccgccgg caggcggttt cgttctcgtg
     7201 ccaacaaggt cggcgacgct gctattccag ctgggcaggg aagtccgctg ctcggccacg
     7261 ccgatatccg catcacgtca cggcacgacg cacacctgcg ctgtcgcacg ctggcgaacg
     7321 cggtgaacgc g
//
LOCUS       DASSVX010000118         7805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_287647, whole genome shotgun sequence.
ACCESSION   DASSVX010000118 DASSVX010000000
VERSION     DASSVX010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287647"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFO53_05255"
     CDS             <1..306
                     /locus_tag="VFO53_05255"
                     /inference="COORDINATES: protein motif:HMM:NF028457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HET8875717.1"
                     /translation="AQQPSQPTTLPDPRSLFIGYHYTFAKLPDEPMHPRPADERVGYF
                     TTEVLDFTSDVPRVPVERYANHWRLEKKDPSAALSEPKTPIVFWLDRNIPVKYREPI"
     gene            373..1971
                     /locus_tag="VFO53_05260"
     CDS             373..1971
                     /locus_tag="VFO53_05260"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HET8875718.1"
                     /translation="MQPDNADFDTSDIRHASVRWQTVAKTSYGAIGPSVVDPRTGEIL
                     DADIAIDANNVRVVRNLRHEYLPPRKDAFAAFAEATSGNAHAQSDAESCNYDDAATEE
                     SAFGMSLLEARGELDPNSPDIDKFVDLFLKNVTMHEVGHTLGLRHNFRASTVYNEAEL
                     SDAAFTAAHGISGSVMEYNPWNLAVKGERQGEYTMSTLGPYDYWAIEYAYKPIDPANE
                     TAELAKIAARSSEPWLAYSTDEDVAYFAIDPAVNQLDLGSDPLAYAKKRLALVHELWQ
                     RTESMTLAPGDSYSILRRNFTRGLNEAGQGALYASKYIGGLTTLRDHAGSGRQPLTPV
                     DATKQRAALALLANEVLSAGSFDFSPSFLRKMTISTFDIDDAQELGRPAPPLDLSIDQ
                     QVLGLQRGVLDTLMSAPIAQRLLNNAAKVDDPKSALKLPELYATLHNAVWSELTTGKD
                     ITLFRRNLQREYATRVADALLRPSGSMPADARALLRVEATRLRADLRHAPTRRMSEEA
                     KAHILEMGAMLDEAQKAPIVRQAV"
     gene            2145..3140
                     /locus_tag="VFO53_05265"
     CDS             2145..3140
                     /locus_tag="VFO53_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET8875719.1"
                     /translation="MTLAPVDNRALANLCGPLDANLRQIEAALDVTIARRGASFSVTG
                     PRRQATHGARALQRFYGDAEKPLSVDDIQLGLVEIAALDPDADAAAAHVRARDADAAG
                     DVSVALPGEAQPLLRTRRADLHGRTPNQIAYLDVIRTHDISFGIGPAGTGKTYLAVAC
                     AVDALERDTVKRIVLVRPAVEAGERLGFLPGDLAQKVDPYLRPLFDALYDLMGFDKAA
                     RLLERQTIELAPLAYMRGRTLNHSFIILDEAQNTTPEQMKMFLTRIGFGTRAVVTGDV
                     TQIDLARGQKSGLIEAERVLKDVRGIGFVRFTSADVVRHPLVQKIIDAYDRDTST"
     gene            3112..3642
                     /gene="ybeY"
                     /locus_tag="VFO53_05270"
     CDS             3112..3642
                     /gene="ybeY"
                     /locus_tag="VFO53_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET8875720.1"
                     /translation="MPTTATRARNAPSSRTPARSRRASAPTAHEPHAVPRLSLAIQST
                     VESADIPKRAALARIAGAALEHDADVTFRFVDGREGRALNRRYRGRDYATNVLTFVYD
                     DGVSLTGDIVLCAPVIAREAREQRKTLRAHYAHLVIHGMLHLQGYDHERDGDAARMEA
                     REIALLRDLGFANPYA"
     gene            3716..4576
                     /locus_tag="VFO53_05275"
     CDS             3716..4576
                     /locus_tag="VFO53_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HET8875721.1"
                     /translation="MATPFDSPDDKPTLLERLSALLTREPEDREELIALLRGAFERRL
                     LDADALSMIEGVLSVSETTVRDIMIPRAQMDCVSIDDSPVEFMPLIIETKHSRFPVIG
                     ESKDDVVGILLAKELLNYYADPDAFSLRETLRPAVFVPESKRLNVLLREFRANRNHIA
                     IVVDEYGGVSGLVTIEDVLEQIVGDIEDEYDFDESEDNVIPEANGRFRVKAQTEIADF
                     NAQFGTAFEDEDFDTVGGLVLKAFGRLPKRGEVTSVGPFRFRVLRADSRRLYTLQVER
                     IAPPSGEDNP"
     gene            4621..6123
                     /gene="lnt"
                     /locus_tag="VFO53_05280"
     CDS             4621..6123
                     /gene="lnt"
                     /locus_tag="VFO53_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET8875722.1"
                     /translation="MPLALAASAGAVTVFAFAPFGVSGVVFVTLALLFGVWLRADSPR
                     HAAQEGFAFGAGLFAAGSSWLTIALVNFGGMAQWLALLAIAVLTAYLALWPALAGYIA
                     VRFTRSGSWQRVLVGAAAFTATEWIRSYLFTGFPWLALGYSQVPDGLARGYAPVGGVY
                     LVTLVVALVGGLIALFADALAQNAARRAWASATVAAGLLAVGVALASVEWTQAAGTPL
                     PVSLVQGNVVQELKFDPKFRTATFDRYLNLVSQSRGRLIVLPESAFPMFSDEIPDAIL
                     LSLIRTAIARDGDVLAGLFTALPPEKGGDEPRYYNTVVALGTSPLQFYRKNHLVPFGE
                     TIPLKPVIGWFIRSVLAIPLADQARGGATQPALSVAGTRVAVDICYEDAFGSELRHGA
                     RDAQLLVNVTNDAWYGRSIAAEQHNQIAAMRALELGRPMLRATNTGITSAIGEDGREI
                     ARLPWFTTGVLEVTVTGRSGETPYLRAGDVAVLVLCAVLIVAPGVRYRRR"
     gene            6161..7090
                     /gene="glyQ"
                     /locus_tag="VFO53_05285"
     CDS             6161..7090
                     /gene="glyQ"
                     /locus_tag="VFO53_05285"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET8875723.1"
                     /translation="MQTFQEIIQTLQHYWADRGCALLQPYDIEVGAGTSHTATFLRAL
                     GPEPWRAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPAPADILELYLGSLAALGF
                     DLTRNDVRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLDCNPITGEITY
                     GLERLAMYLQGKENVFDLAWTTTRDNGDERTLTYGDVYHQNEVEQSTYNFEQSNAPRL
                     TELFAFHEAEAKRLLDARLPLPGYEMILKAAHTFNLLDARGAISVTERAAYIGRIRAL
                     SRQVAQAYVDSREALGFPMLPANLRRATEAAPA"
     gene            7087..>7805
                     /locus_tag="VFO53_05290"
     CDS             7087..>7805
                     /locus_tag="VFO53_05290"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET8875724.1"
                     /translation="MSDDAARHEAQRPATATLAVEIVTEELPPKALKALQAAFADTLA
                     AELSRRHLLADASVATPYATPRRLAVAISQVRRVAPDAEAVDKLMPAKVARDAQGAAT
                     DALRKKLRALGRPHLADALDADRGPDRLYTASDGKGDYVYLRRTLPGMALDRALQEAL
                     DVVLAHLPIPKVMRYAAANSYFTDVAFVRPAHRLVALHGDDVVRVRALGLDAGRVSSG
                     HRFLARADLAIATADAYAPTL"
BASE COUNT         1284 a         2708 c         2597 g         1216 t
ORIGIN      
        1 gcgcagcagc cgtcgcagcc gacgacgctc cccgacccgc gcagcctctt catcggctat
       61 cactacacgt tcgcgaaact gcctgacgag ccgatgcacc cgcgtcccgc cgacgagcgt
      121 gtcggttact tcacgaccga agtgctcgac ttcacgtcgg acgtcccgcg cgtgcctgtc
      181 gagcgctatg ccaatcactg gcggctcgag aagaaggatc cgtcggccgc gctgtccgag
      241 ccgaagaccc cgatcgtgtt ctggctcgac cgcaacatcc cggtgaagta ccgcgagccg
      301 atctaggaag gcatcctcga atggaacaag gcgttcgagc gcatcggctt caaggatgcg
      361 atccacgtcg aggtgcagcc tgacaacgcc gatttcgaca cctccgacat ccgccacgct
      421 tcggtgcgct ggcagacggt ggccaagacg tcgtacggtg ccatcggccc gtcggtggtc
      481 gatccgcgca ccggagaaat cctcgacgcc gacattgcga tcgacgcgaa caacgtgcgc
      541 gtcgtgcgca acctgcgcca cgaatacctg ccgccgcgca aggatgcctt cgctgcgttc
      601 gccgaagcga cgagcggcaa tgcgcatgcg cagagcgacg ccgaaagctg caactacgac
      661 gatgcggcta ccgaggaatc tgcgttcggc atgtcgctgc tcgaggcgcg cggcgagctc
      721 gatcccaaca gccccgacat cgacaagttc gtcgacctgt tcctcaagaa cgtcacgatg
      781 cacgaggtcg gtcacacgct cggcctccgg cacaacttcc gcgcgtctac cgtgtacaac
      841 gaagccgagc tgtccgacgc cgcgttcacc gctgcgcacg ggatctcggg ctcggtgatg
      901 gaatacaacc cgtggaatct cgccgtgaag ggcgagcgcc agggcgagta cacgatgtcg
      961 acgctcggcc cgtacgacta ctgggcgatc gaatacgcgt acaagccgat cgatcccgcg
     1021 aacgagacgg ccgagctcgc gaagatcgcc gcgcgctcga gcgagccgtg gctcgcctac
     1081 tccaccgacg aggacgtcgc ctatttcgcg atcgatccgg cggtgaacca gctcgacctc
     1141 ggcagcgatc cgcttgccta tgcgaaaaag cgtctcgcgc tggtgcacga gctgtggcag
     1201 cgcaccgaga gcatgacgct cgcgcccggc gacagctatt cgatcctgcg ccgcaacttc
     1261 acgcgcgggc tcaacgaagc ggggcagggc gcgctctacg cgtcgaaata catcggcggc
     1321 ctcacgacgc tgcgcgatca cgcgggcagc ggcaggcagc ctctcacgcc ggtcgacgcg
     1381 acgaagcagc gcgcggcgct cgcgctgctg gcgaacgaag tgctgtcggc cggcagcttc
     1441 gacttttcgc cttcgttcct gcgcaagatg acgatctcga cgttcgacat cgacgacgcg
     1501 caggagctgg gtcggccggc gcccccgctc gacctgtcga tcgaccagca ggtcctcggg
     1561 ctgcagcgcg gcgtgctcga cacgctgatg agcgcgccga tcgcgcagcg gctcctgaac
     1621 aacgcggcga aggtcgatga tccgaagagc gcgctcaagc tcccggagct ctacgcgacg
     1681 ctgcacaacg cggtgtggag cgagcttacg accggcaagg acatcacgct gttccggcgc
     1741 aacctgcagc gcgagtatgc gacgcgtgtc gccgacgcgc tgctgcgccc gtcgggatcg
     1801 atgcccgccg acgcgcgcgc gcttttgcgc gtggaggcaa cgcgtcttcg cgccgatctt
     1861 cgccacgcac cgacgcgccg catgtcggag gaggcgaagg cgcatatcct cgagatggga
     1921 gccatgctcg acgaagcgca aaaggcgccg atcgtccgcc aggcggtttg agcccgcccg
     1981 cgcatcgcac caaagccgcc gggcgcgctg cgtctagtgg tggacgcgag ccgcgcgcga
     2041 gcggcgaagg gccgccccga gccgcgagtc acgccccccc ggggggcagc gcggcggcga
     2101 agcggcaagc gtggggggat cacacccggc gcgttaccga aacgctgacg ctcgcgccgg
     2161 tcgacaaccg cgcgctcgcc aatctctgcg ggccgctcga cgccaacctg cgccagatcg
     2221 aagcggcgct cgacgtcacg atcgcgcgtc gcggcgcgtc gttcagcgtc accggtccga
     2281 ggcgccaggc gacgcatggc gcgcgcgcgt tgcagcgctt ctacggcgac gcggaaaagc
     2341 cgctgtccgt cgacgacatc cagctcggcc tcgtggaaat cgccgcactc gatcccgacg
     2401 ccgatgccgc tgcggcccac gtgcgagccc gcgatgccga tgcagccggc gacgtttccg
     2461 tcgcgctgcc gggcgaggct caaccgctgc tgcgcacgcg ccgcgccgac ctgcatggcc
     2521 gtacgccgaa ccagatcgcg tatctcgacg tgatccgaac acacgacatc agcttcggca
     2581 tcggtcccgc aggtaccggc aagacgtacc tcgccgtcgc gtgcgcggtc gacgcgctcg
     2641 agcgcgacac ggtgaagcgc atcgtgctcg tgcgcccggc cgtcgaggcg ggcgagcggc
     2701 tcggcttcct gcccggcgat ctcgcgcaaa aggtcgaccc gtacctgcgg ccgctcttcg
     2761 atgcactcta cgacctgatg ggcttcgaca aggccgcgag gctgctcgag cgccagacga
     2821 tcgagctcgc gccgctcgcg tacatgcgcg ggcgcacgct caaccattcg ttcatcatcc
     2881 tcgacgaggc gcagaacacc acgcccgagc aaatgaagat gttcctgacg cgtatcggct
     2941 tcggcacgcg cgcggtggtc accggcgacg tcacgcagat cgacctcgcg cgcggacaga
     3001 agagcgggtt gatcgaagcc gagcgggtgt tgaaggacgt gcgcggcatc ggtttcgtcc
     3061 gcttcacgag cgccgacgtc gtgcgccatc cgctcgtgca gaaaatcatc gatgcctacg
     3121 accgcgacac gagcacgtaa cgccccctcg tcccgcacgc cagcccgctc gcggcgagca
     3181 agtgcaccga cagcgcacga gccgcacgcc gttccgcggc tttcgctcgc catccagtcg
     3241 acggtcgaaa gcgccgatat cccgaagcgg gcggcacttg ctcgcatcgc aggcgcggca
     3301 ctcgagcacg atgccgatgt cacgttccgg ttcgtcgatg gccgcgaggg ccgtgcgctc
     3361 aatcggcggt accgcggccg cgactacgcc accaacgtcc tgacattcgt gtacgatgac
     3421 ggcgtgtcgc tgacgggcga catcgtcttg tgtgcgccgg tcatcgcaag ggaggcgcgg
     3481 gagcagcgca agacacttcg agcgcattac gcgcacctgg tgatccacgg catgctgcat
     3541 ctgcagggat acgaccacga gcgcgacggc gacgccgcgc ggatggaagc gcgcgaaatc
     3601 gcgttgctgc gcgacctggg attcgcgaac ccctacgcat gaggtccgcg gctcgacctc
     3661 gcatcgtcat gcgcgttggc gctcgtcccg cgcccagcgc cccgcgggcc tcgtgatggc
     3721 gacgccgttc gattcccccg acgacaaacc cacgctcctc gaacggctgt cggcgctgct
     3781 cacgcgcgag ccggaagatc gcgaggagct gatcgcgctt ttgcgcggcg ccttcgagcg
     3841 gcggctgctc gacgccgacg cgctgtcgat gatcgagggc gtgctgtcgg tgtcggagac
     3901 gacggtgcgc gacatcatga ttccgcgcgc gcagatggac tgcgtgtcga tcgacgattc
     3961 accggtcgag ttcatgccgc tcatcatcga gacgaagcat tcgcgcttcc cggtgatcgg
     4021 cgaatcgaag gacgacgtcg tcggcatcct gctcgcgaag gaattgctga actattacgc
     4081 cgatcccgat gcgttcagcc tgcgcgagac gctgcgcccg gcggtgttcg ttcccgaatc
     4141 caagcggctc aacgtgctgc tgcgcgagtt ccgcgccaac cgcaaccaca tcgccatcgt
     4201 cgtcgacgag tacggcggcg tgtcggggct cgtgacgatc gaggatgtgc tcgagcagat
     4261 cgtcggtgat atcgaggacg agtacgactt cgacgagagc gaggacaacg tcattcccga
     4321 ggcgaacggc cgcttccggg tgaaggcgca gacggagatc gccgacttca acgcacagtt
     4381 cggcaccgcg ttcgaagacg aggatttcga taccgtcggc ggcctcgtgc tgaaggcgtt
     4441 cggccgcctg ccgaagcgcg gcgaagtgac gagcgtcggt ccgttccgct tccgggtgct
     4501 tcgcgccgac agccggcgcc tctacacgct tcaggtcgaa cgcatcgcgc cgccgtccgg
     4561 cgaagacaat ccgtgattcg cgcggaatga gcggggggac gcattagcgc cgcgcgcgcc
     4621 ttgccgctcg cgctcgccgc gtcggcgggg gcagtcacgg tgttcgcgtt cgcgccgttc
     4681 ggcgtgtcgg gcgtcgtctt cgtcacgctg gcactgctgt tcggcgtgtg gctgcgcgcc
     4741 gattccccgc ggcatgccgc gcaggaaggc ttcgcgttcg gcgccggctt gttcgccgcc
     4801 gggtcgtcgt ggctcacgat cgcgctcgtc aacttcggcg gcatggcgca gtggcttgcg
     4861 ctgctcgcga tcgcggtcct caccgcctac ctcgcgctgt ggccggcgct ggccggctac
     4921 atcgccgtgc gcttcacgcg aagcggctcg tggcagcgcg tgctcgtcgg agccgccgcg
     4981 ttcaccgcga ccgaatggat tcgcagctac ctgttcaccg gctttccgtg gctcgcactc
     5041 gggtactcgc aggtgcccga cggactcgcg cgcggctatg cgccggtagg tggcgtgtat
     5101 ctcgtcacgc tggtcgtcgc actcgttggc gggctgatcg cactcttcgc cgatgcgctg
     5161 gcgcagaatg ccgcgcgccg cgcatgggct tcggcgacgg tagcggcggg gctgctggcg
     5221 gtcggcgtcg cgctggcgag cgtcgaatgg acgcaggcgg cggggacgcc gcttccggtg
     5281 tcgctggtgc agggcaatgt cgtgcaggag ctgaaattcg atcccaagtt ccgcacggcg
     5341 acgttcgatc gctacctgaa cctcgtgagc cagagccgcg gccggctcat cgtgttgccc
     5401 gagagcgcgt ttccgatgtt ctcggacgaa attcccgatg cgatcctgtt gtcgctgatc
     5461 cgcacggcga tcgcgcgcga cggcgacgtg ctcgccgggc tcttcaccgc gctgccgccc
     5521 gagaagggcg gcgacgagcc gcgctactac aacacggtcg tggcactcgg cacgagcccg
     5581 ctgcagtttt accggaagaa tcatctcgtg ccgttcggag aaacgatccc gctgaagccg
     5641 gtgatcggct ggttcatccg ctcggtgctc gccattccgc tcgccgacca ggcgcgcggc
     5701 ggcgcaacgc aacccgcgct gtcggtggcc ggcacacgag tcgccgtgga catctgctac
     5761 gaggacgcgt tcggcagcga gctgcgccac ggcgcccgcg atgcgcagct gctcgtcaac
     5821 gtgaccaacg atgcttggta cggacgctcg atcgccgccg agcagcacaa ccagatcgcc
     5881 gcgatgcgcg cactcgagct cgggcggccg atgcttcgcg cgacgaacac cggtatcacg
     5941 tcggcgatcg gcgaggacgg ccgcgaaatc gcgcggctgc cgtggttcac gaccggcgtg
     6001 ctcgaagtca ccgtcaccgg ccgcagcggt gaaactccgt atcttcgcgc cggcgacgtt
     6061 gcggtgctcg tgctgtgcgc ggtgctgatc gtcgcgcccg gcgtgcgtta ccggcgaagg
     6121 tagaatcgtg tcaggtcgaa gcacgcaagg cgcggtcacg atgcagacgt tccaggagat
     6181 catccagacg ctccagcact actgggccga tcgcggctgc gcactgctgc aaccgtacga
     6241 catcgaagtg ggcgccggca cgtcgcacac ggcgacgttc ctgcgcgcac tcggccccga
     6301 gccgtggcgc gcggcctacg tgcagccgtc gcgccgcccg aaggacggcc gctacggcga
     6361 gaaccccaat cggctgcagc attactacca gtaccaggtc gtgctgaagc cggcacccgc
     6421 agacatcctc gagctctatc tcggctcgct cgcggcgctc ggcttcgatc tcacgcgaaa
     6481 cgacgtgcgc ttcgtcgagg acgattggga gaaccccacg ctcggcgcct gggggctcgg
     6541 ctgggaagtg tggctgaacg gcatggaagt gacgcagttc acctacttcc agcaggtggg
     6601 cggactcgat tgcaatccga tcaccggcga gatcacctac ggactcgagc ggctcgcgat
     6661 gtacctgcag ggcaaggaga acgtgttcga cctcgcgtgg acgacgacgc gcgacaacgg
     6721 cgacgagcgc acgttgacgt acggcgacgt ctatcaccag aacgaggtcg agcaatcgac
     6781 gtacaacttc gagcagtcga acgcgccgcg gctgaccgag ctcttcgcgt tccacgaggc
     6841 cgaggcgaag cgcctgctcg atgcgcgcct gccgcttccc ggctatgaaa tgattctcaa
     6901 ggcggcgcac acgttcaacc tgctcgacgc gcgcggcgct atctcggtca ccgagcgggc
     6961 ggcgtacatc ggacgcatcc gcgcattgtc ccggcaggtc gcgcaggcgt acgtcgattc
     7021 gcgcgaggcg ctcggcttcc cgatgctgcc cgccaacctc cggcgtgcaa ccgaagccgc
     7081 gccggcatga gcgacgacgc ggcacgacac gaagcgcagc ggccggccac ggcaacgctt
     7141 gccgtcgaga tcgtcaccga ggagttgccg ccgaaggcgc tgaaggcgct ccaagcggcc
     7201 ttcgccgata cgctcgccgc cgaattgtcg cgacgccatc tgctggccga cgcgagcgtc
     7261 gcgacgccgt atgcgacacc gcgccggctc gccgtggcga tctcgcaggt gcggcgtgta
     7321 gcgcccgatg cggaagcggt cgacaagctg atgccggcga aggtcgcgcg cgatgcacag
     7381 ggcgcggcca ccgacgcgct gcgaaagaag ctccgcgcgc tcggacgccc gcatctcgcc
     7441 gacgccctcg atgccgatcg cggccccgat cggctctaca cggcgtccga cgggaagggc
     7501 gactacgtct atctgcgccg caccctgccg ggcatggcgc tcgatcgcgc gttgcaggaa
     7561 gcgctcgacg tggtgctcgc gcacctcccg atccccaagg tcatgcgcta cgcggcagcg
     7621 aacagctact tcaccgacgt cgccttcgtg cgtccggcgc accggctcgt ggcgctgcac
     7681 ggtgacgatg tcgtccgcgt gcgtgcgctc gggctcgatg cgggccgcgt gagctcgggt
     7741 caccgctttc tcgcccgtgc ggatcttgcg atcgccaccg ccgacgccta tgcgccgacg
     7801 ctcga
//
LOCUS       DASSVX010000119         7280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_220930, whole genome shotgun sequence.
ACCESSION   DASSVX010000119 DASSVX010000000
VERSION     DASSVX010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220930"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFO53_05295"
     CDS             <1..531
                     /locus_tag="VFO53_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8875725.1"
                     /translation="VEKLLERTRAWTYGDPSDPKVDMGTVIDEAAARNFEAKVDAAVA
                     QGAWLLHGNRREAALYSPTVLDHVSPDMDVVRTETFGPVSPVIRFRDIDEAIRISNST
                     AYGLSSSVCTNRLDYITRFVRELQVGTVNVREVPGYRLELTPFGGIKDSGLGYKEGVQ
                     EAMKSFTNVKTYSLPW"
     gene            545..2266
                     /locus_tag="VFO53_05300"
     CDS             545..2266
                     /locus_tag="VFO53_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET8875726.1"
                     /translation="MQTLLNLLAGVSLLVWGTHIVRTGILRMYGGDLRRVLRRSVSKR
                     VSAFFAGLGVTGLIQSSTATALIVAAFAGQGLIGTAPALAVMLGADVGTALVTVLLSF
                     DLSWLAPLLIFSGIVLYLSSQASNAGRFGRILIGLGLIMFALQWISVAAHPIVQAAGV
                     KVIFASLTGDVMLDMLVAALFTVLCYSSLAVVLLVATLASLHVIALPVALGLVLGANL
                     GSGVLGMLSTLRAAPEARRVTLGNFLFKLIGCVLAVPLAGHLDEWIGGIGLDAAREVV
                     LFHLAFNVVLALLLLYWTEHIAKIAEQLLPARPLSEDPAKPRHLDPSALDTPGLAIAC
                     AAREAMRIGDVVEQMLAGMLTVLTTNDRTLAEHLRKTDDIVDELYTAVKLYLAQIPRE
                     ALEQREGRRWADIVSFAINMEQVGDIIERILTDLEDKKIDKGRNFSEAGMAEICDLHA
                     RLIANLRLGLSVFLNGDLKHAQELLAQKVLFRDLERAYANSHLGRLAGNTVESIETSS
                     LHLDLISDLRRINSHICSIAYPILEEAGVLARTRLKTDESVAAPADRAAPLGDGAAWQ
                     KRKPKTA"
     gene            2274..3134
                     /locus_tag="VFO53_05305"
     CDS             2274..3134
                     /locus_tag="VFO53_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET8875727.1"
                     /translation="MVMPAQELSLGITLAVLGAGLLHAGWNALLKGAPGGDPMLDTAT
                     VVAGSCLCALVVLPFVPLPDPSAWKFIAGSTVIHFAYYVTLAQAYRTGDLSFAYPLMR
                     GSAPLIVAVLGVVFLHELPTTHAMLGIVLICTGIVSIAFVRRERHPPSAARWAFANAA
                     IIAAYTLVDGSGARASGNALAYVTWLIFIEGLPFIAWILRRRGAAGAAYLQRGWRRGL
                     VGGAASLAAYGIVLFAMTRAPVAAVAALRETSVLFAALIGALWLKEGFGAMRLAGAAS
                     VAVGIAALKL"
     gene            3143..5572
                     /locus_tag="VFO53_05310"
     CDS             3143..5572
                     /locus_tag="VFO53_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8875728.1"
                     /translation="MNVPTHARAVVVGGGIAGASAAYHLTKLGLTDVVLLEQGRLTCG
                     TTWHAAGLVGQMRATRNATRMSRYGIELYASLERETGLATGWKQCGSVNVAKTRERMQ
                     LMRRQMARARSFGVEFAWLAPQEIAAQLPLLRTDDLEGGVWIPGDGKANPTDLTQSLA
                     KGARARGARIVEGAKVTGVGAHNRHVSGVQWTAGDARGEIVCEFVVNCAGQWAREFGA
                     LAGVAVPLASAEHFYLVTEPIDGVTTDMPVIRDPDGYIYFKEEVGGVVMGGFEPVAKP
                     WNVERIPERFEFQLLPEDWDQFEVLMQTALHRMPCLETAPVKLLLNGPESFTLDGNFL
                     LGEAPELRGYFVCAGFNSSGIANAGGAGRLVAEWLVGGEPPFDAWDVDIRRFAPLHVN
                     RRHLADRTVETLGLHYAMRWPREELSSVRPLRRSPLHDRLRDKRAVFGTKNNWERANY
                     FLPERASEPPPTLDTPGWLAWMLDEQRACREEVVVFDQASFSKFVLKGRDALAVLERL
                     CANRIDVPVGRIVYTALLNARGGFESDLTITRVAANEFFILTGTAQTTRDFGWIESHI
                     GDDEHASLVDVTSAWSILSLMGPRAEALLSSLSRDDVSKAGLPSGTTRIVDVGYARAR
                     AARISYVGGPGYELCVPTDQCVTLYDALGSEGMRFGLRDAGYYTIDALRIEAGRRAWG
                     AELSPDETPLEAGLAHAVAFDKPQFIGRDALRRQRAHGVDKRLVQFTLDDPAAFAWGG
                     EPILIDAVPAGEITSAGYSRKLGRSVAFGYVKSRAAEVPLSDAQILGARYAIDIAGTE
                     YAATAHLQAPR"
     gene            5599..6435
                     /locus_tag="VFO53_05315"
     CDS             5599..6435
                     /locus_tag="VFO53_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HET8875729.1"
                     /translation="MTTVHRLTPPPRGRTPSRPGSATAQVQSLTRGLSILECLARSEG
                     GLTLTDIAHRVELPASTTHRLLGTLEKTGFVYQMGDLGRWYVGLSAFTVGSSFLANRD
                     FATHSHAYMRRLMEQCGETANLAILDGTEAVFIDQVQCRETMRTIVKLGSRVPLHASG
                     VGKAIFASLPDDQIDALIKVRGLPRITENTITSPETMWASIRVIRQRGFSFDDEEHLP
                     GTRCVAAPIYDEHAEPLGAISLAGPSSRLPDPRIKQLGPLVSRIAEEITGRLGGRWPH
                     PR"
     gene            6485..>7280
                     /locus_tag="VFO53_05320"
     CDS             6485..>7280
                     /locus_tag="VFO53_05320"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET8875730.1"
                     /translation="MNPAPRLHGALAPVVTPFTADLRPDAGRLARHCRWLLAQETGLA
                     VFGTNSEGNSLTVPEKLGLLDALVAAGIPPQRMMPGTGCCALPDTVELTRHAMRHGCG
                     GVLMLPPFYYKGVSDDGLFASYSDVIERVGDARLRIYLYHIPQVSQVPIGLPLIERLL
                     ARYPGTIAGIKDSSGDFDNTRAMLERFEAQGFDVFSGSESFLLATLRAGGAGCISAIA
                     NVNPGPIARLVREWQNRDADAQQAALDGVRAVYQSFSMLPALKATIA"
BASE COUNT         1101 a         2529 c         2437 g         1213 t
ORIGIN      
        1 gtcgaaaagc tgctcgagcg cacgcgggcc tggacgtacg gcgatccttc cgaccccaag
       61 gtcgacatgg gaaccgtgat cgacgaggcg gccgcgcgca atttcgaggc gaaggtcgac
      121 gccgccgtcg cgcagggcgc atggcttttg catggcaacc ggcgcgaagc cgcgctctac
      181 tcgcccacgg tgctcgacca cgtcagcccg gacatggacg tggtgcgcac cgaaacgttc
      241 gggccggtgt cgccggtgat ccgctttcgc gacatcgacg aggcaatccg gatctcgaat
      301 tcgaccgcgt acgggctctc gtcgtcggta tgcaccaatc ggctggacta catcacgcgg
      361 ttcgtccgcg aactgcaggt cggaacggtg aacgtgcgcg aggtccccgg gtatcggctc
      421 gagctgacgc cgttcggcgg catcaaggac tcggggctcg gctacaagga aggagttcag
      481 gaggcgatga agagcttcac caacgtcaag acgtactcac taccctggtg agtgacgcgt
      541 agtcgtgcag acattgctca acctgctggc cggcgtgtcg ctgctcgtat ggggcacgca
      601 catcgtgcgc accggcatcc tgcggatgta cggcggcgac ctgcgccgcg tgctccggcg
      661 cagcgtgagc aagcgcgtgt cggcgttctt cgccgggctc ggcgtcaccg gtctcatcca
      721 gagcagcacc gccacggcgc tgatcgtggc cgcgtttgcg ggacaggggc tgatcggcac
      781 cgcacccgca ctcgccgtca tgctcggcgc cgacgtgggc accgcgctcg tcacggtgct
      841 gctgtcgttc gacctgtcgt ggctcgcgcc gcttttgatc ttcagcggca tcgtcctcta
      901 cctgtcgagc caggccagca acgcgggacg cttcggccgc atcctgatcg gcctcgggct
      961 catcatgttc gcgctgcagt ggatttcggt cgcggcgcat ccgatcgtgc aggcggccgg
     1021 cgtcaaggtg atcttcgcgt cgctcaccgg cgacgtgatg ctcgacatgc tggtggccgc
     1081 gcttttcacc gtgctgtgct attcgagcct cgcggtggtg ctgctcgtcg cgacactcgc
     1141 atcgctgcac gtcatcgcgc tgccggtagc actcggtctc gtgctcggcg cgaatctggg
     1201 cagcggcgtg ctcggcatgc tgtcgacgct gcgcgccgca cccgaagcgc ggcgcgtcac
     1261 gctcggcaac ttcctgttca agctgatcgg ctgcgtgctc gccgtcccgc tcgccggcca
     1321 tctcgacgag tggatcggcg gcatcggcct cgacgcggcg cgtgaggtcg tgctgttcca
     1381 cctcgcgttc aacgtcgtgc tggcactgct gctgctgtac tggaccgagc acatcgcgaa
     1441 gatcgccgag caactgctgc cggcgcgtcc gctgtccgag gacccggcga agccgcgcca
     1501 tctcgacccg tcggcgctcg acacgccggg cctcgccatc gcctgtgccg cgcgcgaggc
     1561 gatgcgcatc ggcgacgtgg tcgagcagat gctcgccggg atgctgaccg tcctgacgac
     1621 gaacgatcgc acgctcgccg agcacctgcg caagaccgac gacatcgtcg acgagctcta
     1681 tacggcggtg aagctctacc tcgcgcagat tccgcgcgag gcgctcgagc agcgcgaagg
     1741 ccgccgctgg gccgacatcg tgtcgttcgc gatcaacatg gaacaggtcg gcgacatcat
     1801 cgagcgcatc ctgaccgacc tcgaggacaa gaagatcgac aagggccgca atttctccga
     1861 ggccggcatg gccgagatct gcgacctgca tgcgcggctg atcgcgaacc tgcgcctcgg
     1921 gctgtcggtg ttcctgaacg gcgacctgaa gcatgcgcag gagctgctcg cgcagaaggt
     1981 gctgttccgc gacctcgagc gcgcgtatgc gaactcgcac ctcggtcgtc tcgccggtaa
     2041 cacggtcgag agcatcgaga cgtcgtcgct gcatctcgac ctcatttccg atctgcggcg
     2101 gatcaattcg cacatctgct cgatcgccta tccgatcctc gaggaagcgg gcgtgcttgc
     2161 ccgcacgcgg ctcaagaccg acgaaagcgt cgccgcaccg gccgatcgcg ctgcgccgct
     2221 cggcgacggc gccgcatggc agaagcgcaa gccgaagacc gcatgattcc cggatggtga
     2281 tgccggcgca ggagctgtcg ctcggcatca cgctcgccgt cctcggcgcc ggcctgctgc
     2341 acgcaggctg gaacgcgctg ctgaagggcg cgcccggcgg cgatccgatg ctcgacacgg
     2401 cgaccgtcgt cgccggctcg tgcctgtgcg cgctcgtcgt ccttccgttc gtgccgctgc
     2461 ccgacccttc cgcgtggaaa ttcatcgcgg gttccacggt gatccacttc gcgtattacg
     2521 tgacgctcgc acaggcgtac cgcaccggcg acctgtcgtt cgcctatccg ctgatgcgcg
     2581 gcagcgcgcc gctgatcgtc gcggtgctcg gcgtcgtgtt cctgcacgag cttccgacca
     2641 cgcatgcgat gctcggcatc gtgctgatct gcaccggcat cgtcagcatc gccttcgtgc
     2701 gccgcgagcg tcatccgccg tcggccgccc ggtgggcgtt cgccaatgcc gcgatcatcg
     2761 cagcctatac gctcgtcgac ggcagcggcg cgcgcgcgtc gggcaacgcg ctcgcctacg
     2821 tcacctggct gatcttcatc gaaggcctgc cgttcatcgc atggatcctc cggcggcgcg
     2881 gcgccgccgg ggccgcctac ctgcagcgcg gctggcgtcg cggactcgtc gggggcgcgg
     2941 cgagcctcgc cgcgtacggc atcgtgctgt ttgcgatgac gcgcgcgccg gtcgccgccg
     3001 tcgccgcgct gcgcgaaacg tcggtgctgt tcgccgcgct gatcggcgcg ctgtggctga
     3061 aggagggttt tggcgcgatg cgcctggcgg gtgccgccag cgtggcggtg ggcatcgccg
     3121 cactgaaact gtaaggcgcg gcgtgaacgt ccccacccat gctcgcgcgg tggtcgtcgg
     3181 cggcggcatt gccggtgcgt cggcggcgta tcacctgacg aagctcgggc tcaccgacgt
     3241 cgtgctcctc gagcagggac gcctcacgtg cggcacgacc tggcacgcgg ccggcctcgt
     3301 cggccagatg cgcgccacgc gcaacgcgac gcggatgagc cgctacggca tcgagctcta
     3361 tgcgtcgctc gagcgcgaaa ccggcctcgc caccggatgg aagcaatgcg gtagcgtgaa
     3421 tgtcgcgaaa acgcgcgagc gcatgcagct gatgcgccgg cagatggcgc gcgcacgcag
     3481 cttcggcgtc gaattcgcat ggctcgcgcc gcaggagatc gccgcgcagc tgccgctgct
     3541 tcgcaccgac gatctcgaag gcggtgtgtg gattccgggc gacggcaagg cgaatccgac
     3601 cgatctcacg caatcgcttg cgaagggcgc acgggcgcgc ggcgcacgca tcgtcgaagg
     3661 cgcgaaggtg acgggcgtcg gcgcgcacaa ccggcatgtc agcggcgtgc agtggacggc
     3721 gggtgacgcg cgcggcgaga tcgtctgcga gttcgtggtc aattgcgccg gccagtgggc
     3781 gcgcgaattc ggcgcgctcg ccggcgtcgc ggtgccgctt gcatcggccg agcatttcta
     3841 tctcgtcacc gagccgatcg acggtgtcac gaccgacatg ccggtgattc gcgatcccga
     3901 cggctacatc tacttcaagg aggaagtcgg cggcgtcgtg atgggcggct tcgagccggt
     3961 cgccaagcca tggaacgtcg agcgcattcc cgagcggttc gagttccagc tgctgcccga
     4021 ggactgggac cagttcgagg tgctgatgca aaccgcgctg caccggatgc cctgtctcga
     4081 aacggcgccg gtcaagctgc tgctgaacgg gccggaaagc ttcacgctcg acggcaactt
     4141 cctgctcggc gaggcacccg agttgcgcgg ctatttcgtt tgcgccggct tcaattcgtc
     4201 gggcattgcc aacgcgggcg gtgcaggacg gctcgtcgcc gaatggctgg tcggcggcga
     4261 gccgccgttc gacgcatggg acgtcgacat tcgccgcttc gcaccgctgc acgtcaaccg
     4321 gcgccatctc gccgatcgca cggtcgaaac gctcggcctg cactatgcga tgcgctggcc
     4381 gcgcgaggag ctttcgtcgg tgcggccgct gcggcgctcg ccgctccatg accggctgcg
     4441 cgacaaacgc gccgtgttcg gcacgaagaa caactgggaa cgcgccaact acttccttcc
     4501 cgagcgcgcg agcgagccgc cgcccacgct cgatacgccg ggctggctcg catggatgct
     4561 cgacgagcag cgcgcctgtc gcgaagaggt cgtcgtcttc gaccaggcgt cgttttccaa
     4621 attcgtgctg aagggccgcg atgcgcttgc ggttctcgag cggctctgcg caaaccgcat
     4681 cgacgtcccg gtcgggcgca tcgtctatac cgcgctcctc aacgcccgcg gcggattcga
     4741 gagcgatctc acgatcacgc gcgtggccgc gaacgaattc ttcatcctca ccggcaccgc
     4801 gcagacgacg cgcgatttcg gatggatcga aagccacatc ggcgacgacg agcatgcgtc
     4861 gctcgtcgac gtgacgagcg catggtcgat cctgtcgctg atgggaccgc gcgccgaagc
     4921 gctgctgtcg tcgctgtcgc gtgatgacgt gtccaaggct gggctgccgt ccggcacgac
     4981 acgcatcgtc gacgtcggtt acgcacgcgc ccgtgccgca cgcatcagct acgtcggcgg
     5041 tcccggttac gagctctgcg tgcccaccga ccagtgcgtg acgctctacg acgcactagg
     5101 cagcgaaggc atgcgtttcg ggctgcgcga cgccggctat tacacgatcg acgcgttgcg
     5161 catcgaggcg gggcggcgtg cctggggcgc ggagctgtcg cccgacgaga cgccgctcga
     5221 ggcaggtctc gcgcacgcgg ttgcgttcga caagccgcaa ttcatcggcc gcgacgcgtt
     5281 gcgccggcag cgcgcgcacg gggtggacaa gcggctcgtc cagttcacgc tcgacgaccc
     5341 ggccgcgttc gcgtggggcg gcgagccgat cctgatcgac gccgtgccgg ccggcgaaat
     5401 cacgtcggcg ggctacagcc gcaagctcgg ccgcagcgtc gcattcggct acgtgaaatc
     5461 gcgcgcggcg gaggtcccgc tttccgacgc gcagatcctc ggtgcgcgtt acgcgatcga
     5521 catcgcgggc accgaatacg cagcgacggc ccatctgcag gcgccgcgct agactagcgc
     5581 tgcccgttcc ccatcgccgt gacgaccgtc catcgcctga ccccgccacc gcgcggccgc
     5641 acgccgtcgc gccccggcag cgcaaccgcg caggtgcaaa gcctcacgcg cgggctgtcg
     5701 atcctcgaat gtctcgcacg cagcgaaggc ggattgacgc tcaccgacat cgcgcatcgg
     5761 gtcgagttgc ccgcgtcgac gacgcatcgg ctgctcggca cgctggagaa gaccgggttc
     5821 gtttaccaga tgggcgacct cggacgctgg tacgtgggcc tttccgcgtt caccgtcggc
     5881 tcgagcttcc ttgccaaccg cgacttcgcg acgcacagtc atgcgtacat gcgccggctg
     5941 atggagcaat gcggcgaaac ggcgaacctc gcgatcctcg acggtaccga agcggtgttc
     6001 atcgaccagg tgcagtgccg cgagacgatg cgcacgatcg tgaagctcgg cagccgcgtg
     6061 ccgctgcacg cgtcgggcgt cggcaaggcg atcttcgcgt cgctgcccga cgaccagatc
     6121 gacgcgctga tcaaggtcag gggcctgccg cgcatcaccg agaacacgat cacgtcgccc
     6181 gagacgatgt gggccagcat tcgcgtgatc cgccagcgcg gcttttcgtt cgacgacgag
     6241 gagcacctgc ccggcacgcg ctgcgtcgca gcccccatct acgacgagca tgccgagccg
     6301 ctcggcgcga tttcgctcgc cggtccgtcg tcgcgactgc ccgacccccg catcaagcag
     6361 ctcggcccgc tcgtgtcgcg catcgccgag gaaatcaccg gcaggctcgg cggccgctgg
     6421 ccgcacccgc gctgacgcgt cggcgccacg tcgcatttat ccgcgcatgc gaaaggagag
     6481 cgccttgaac ccggccccac gtctgcacgg cgcgcttgcg ccggtcgtca cgccgttcac
     6541 cgccgatctg cgtcccgatg cgggccgtct cgcccgccac tgccgctggc ttctcgcaca
     6601 ggagacgggc ctcgcagtat tcggcaccaa ttccgaaggc aactcgctga cggtgcccga
     6661 aaagctcggt cttctcgacg cactcgtcgc cgcgggcatt ccaccgcagc gcatgatgcc
     6721 gggcaccggc tgttgcgcgc tgcccgacac tgtcgagctg acgcgccacg caatgcgcca
     6781 cggctgtggc ggcgtgctga tgctgccgcc gttctattac aagggcgtgt ccgacgacgg
     6841 gctcttcgcg agctattccg acgtgatcga gcgcgtcggc gacgcgcggc ttcgcatcta
     6901 cctctatcac atcccgcagg tgtcgcaggt gccgatcggc ctcccgctga tcgagcgcct
     6961 gctggcgcgc tacccgggca cgatcgcggg catcaaggac agctcgggcg atttcgacaa
     7021 tacgcgcgcg atgctcgagc gctttgaggc gcagggcttc gacgtctttt cgggcagcga
     7081 aagcttcctg ctcgcgacgt tgcgcgcggg tggcgccggg tgcatcagcg ccatcgccaa
     7141 cgtcaatcca ggacccatcg cccgcctcgt ccgcgaatgg cagaaccgcg atgccgacgc
     7201 gcagcaggcg gcgctcgacg gcgtgcgcgc cgtctaccaa tcgttttcca tgctgccggc
     7261 gttgaaggcg acgatcgccc
//
LOCUS       DASSVX010000120         5531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1020995, whole genome shotgun sequence.
ACCESSION   DASSVX010000120 DASSVX010000000
VERSION     DASSVX010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020995"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="VFO53_05325"
     CDS             complement(<1..1108)
                     /locus_tag="VFO53_05325"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8875731.1"
                     /translation="MSSDLAYRSAAELIALYRAKHASPVEVTRACLARIEALNPALNA
                     FCIVDAEGALRAAGESEARWMRGAPQGLLDGVPVSIKDLLLTTGWPTLRGSRTVDANQ
                     AWNQDAPAVARLREHHAIMLGKTTTPEFGWKAVTDSPLTGITRNPWNPARTPGGSSGG
                     AAVAVATGMGALAVGTDGGGSIRIPASFTGIFGLKPSFGRVPAWPLSLFGTVSHVGPM
                     TRSVEDAALMLDVISEPDIRDWFALPYDARDYRDGLNDGIAGLRMAFAPTLGGAQVDA
                     EVGDIVERAVAAFAELGADVVVAAPELGDVANVGRTHWWAGATRVVHSMAPERRALAD
                     PGLLRIAEKGSGFTLADYLEAVEARGAIGAALRQF"
     gene            complement(1126..2778)
                     /locus_tag="VFO53_05330"
     CDS             complement(1126..2778)
                     /locus_tag="VFO53_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875732.1"
                     /translation="MSETILDIERLTVDLPSWADRPQAVTGVSLALRRNEILCVVGES
                     GSGKSVMARAIMGLLPAPHLRASAGRILLDGEDLLRADAARMRAIRCRRISMIFQEPM
                     TALNPVKTLGAQIDEVLAVHTALPRRARTERIAAMLESVRLSDAQHLMRAYPHEISGG
                     QRQRAMIAMALILEPSVLIADEPTTALDVTTQAGILALIRDVQRSRHMGVVFITHDFG
                     VVAEIADRVAVMQQGRVVEFGDAPQVLNAPAHPYTQALIAAIPGLTPMTRPANETAPL
                     ALTVRALDKTYRASGSFGGRDRVVRALKSVELAIRHGETLGVVGESGSGKSTLARCIV
                     RLVHADNGSVVVRDVDWMQLSRRRLRAHRRYVQIVFQDPFGSLDPRCSVGRLIAEGPI
                     VCGVSEADALARAYALLELVGLDRRAADRYPHEFSGGQRQRIGIARALALDPAILVAD
                     EPVSALDVSVQAQVLRLLADIRDRFNLAMLFITHDLRVAAQVCDRIAVMRDGEIVEQG
                     ETARVYADPQHAYTRALLAAVPGRHWQASAVLAAERRSASRI"
     gene            complement(2782..3630)
                     /locus_tag="VFO53_05335"
     CDS             complement(2782..3630)
                     /locus_tag="VFO53_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875733.1"
                     /translation="MSAKADVLAFCQRYARNRAALVALGVLVAIVVVAIAGPHLLRHA
                     GPFELAGPPFQPPFGRFALGTDMLGRDELVGIVFGARTSLVIGLVATLFATLLGVLLG
                     GIAGYYGGVADEALMRLTEIFQTIPSFLFSILVVAILSPSIESVIIAIAVVSWPPMAR
                     LVRGEFRSLAGREFVQACVSMGMSDLRVIALHILPNCLSSIIVTGSLLVATAILTESA
                     LSFLGLSDPNVMSWGFMVGAARDFLQRAWWLCAIPGVAILVTVLAINLVGDGLNDALN
                     PRLRHL"
     gene            complement(3713..4699)
                     /locus_tag="VFO53_05340"
     CDS             complement(3713..4699)
                     /locus_tag="VFO53_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875734.1"
                     /translation="MEVARGRARYIGRRLVQAVPTILVIVTLNFLLVHLAPGNAADVL
                     AGESGSATPQYMAYLRHQFGLDQPLYVQYLDYLKNVVTLELGYSFREGMAVRDLILQR
                     LGPTLLLMGTTIVLSIGVGILLGLVAAGRPNSLRDNAISIFALVAYATPLFWVGLMFI
                     LLFSIQLGWLPTSGMTNIAAFYTGWRKAGDVARHLVLPALTLSLFYMALYVRLMRSSM
                     LEQSGMDYVVTARSKGLTERTITWRHVLRNACLPVVTMAGVQVGNLLGGSVVVETVFG
                     WPGIGLLAFRALFARDLNLLLGIFFVAACLVIAVNIIVDVIYSVLDPRIRLR"
     gene            complement(4732..>5531)
                     /locus_tag="VFO53_05345"
     CDS             complement(4732..>5531)
                     /locus_tag="VFO53_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8875735.1"
                     /translation="TTKGYEMQSPIVHLDFNTRVKPFDDVRVRQAISFAIDRKFVIDN
                     VWFGFGKPATGPISSNFAPSGIYTPDVKNYNVPNGADVANKMLDEAGYKRKTDGTRFE
                     IVHDITPYGDEWQRYGEYVKQALAKIGVKATLRYEDVPTWLRRIYTDYDFQLTSNWVQ
                     NLADPVIGIDRLYSSWSIKKGTVFVNDSGWSTPETDRLMKEAAVETDPKKRGALYHQF
                     QKLTVEAAPIVWVMELNFVSVYDSKFKDLTVSPLGTYAAFNKASLAA"
BASE COUNT         1000 a         1863 c         1844 g          824 t
ORIGIN      
        1 ggaactggcg caatgctgcg ccgatcgcgc cacgcgcctc gaccgcttcg agatagtcgg
       61 cgagcgtgaa cccgctgcct ttctcggcga tgcgcaacag cccggggtcg gccaatgcgc
      121 gtcgttccgg ggccatcgag tgcaccacgc gcgtcgcgcc ggcccaccag tgcgtgcgtc
      181 ctacgtttgc gacgtcgcca agctccggcg cggccacgac gacgtcggcg ccaagctcgg
      241 cgaatgccgc caccgcgcgc tcgacgatgt cgccgacctc cgcatcgact tgcgcaccgc
      301 caagcgtcgg cgcgaaggcc atgcgcaggc cggcgatgcc gtcgttcaat ccgtcgcggt
      361 aatcccgagc atcgtaggga agcgcgaacc agtcgcgaat gtcgggctcg ctgatcacgt
      421 cgagcatcag cgccgcatcc tcgacgctgc gcgtcatcgg ccctacgtgc gagacggtgc
      481 cgaacaggct gagcggccag gccggaacgc gcccgaagct cggcttcaat ccgaagatgc
      541 cggtgaatga cgccgggatc cggatcgatc caccgccgtc ggtaccgacg gcgagtgcgc
      601 ccatgccggt cgcgaccgcg actgccgcgc ccccgctgct gccgccgggt gtcctggcgg
      661 gattccaggg attgcgagtg atcccggtga gcgggctgtc cgtcaccgct ttccagccga
      721 actcgggcgt cgtagtcttg ccaagcatga tcgcatggtg ctcgcgcaag cgcgccacgg
      781 ccggcgcgtc ctgattccac gcctgattcg cgtcgacggt gcgcgagccg cgcaacgtcg
      841 gccatccggt cgtcagcagc aaatccttga tcgagaccgg cacgccgtcc agcaaccctt
      901 gcggcgcgcc acgcatccag cgcgcctcgg actcgcccgc cgcgcgcagc gcgccttcgg
      961 cgtcgacgat gcaaaacgca ttgagtgccg gattcaatgc ctcgatgcgc gcaagacatg
     1021 cacgcgtcac ttcgacgggc gatgcgtgct tcgcgcgata aagcgcgatc agctcggctg
     1081 cagagcggta ggcgagatcg gaggacatgg caatggatga tagtgctaaa tgcgcgatgc
     1141 cgaacggcgt tcggccgcga gaacggccga tgcctgccag tgccgccccg gcacggccgc
     1201 cagcagcgcc cgcgtgtacg cgtgctgcgg atcggcgtag acgcgcgccg tctcgccctg
     1261 ctcgacgatt tcgccgtcgc gcatgacggc gatgcggtcg catacctgcg cggcgacgcg
     1321 caggtcatgc gtgatgaaaa gcatcgcgag gttgaagcga tcgcggatat cggcgagcag
     1381 gcgcaacacc tgcgcctgca ccgacacgtc gagtgccgac accggctcgt cggccacgag
     1441 gatcgccggg tcgagcgcga gcgcgcgcgc aatgccgatc cgctgccgct gcccgcccga
     1501 gaattcgtgc ggatagcggt ccgcggcacg ccggtcgagg ccgaccagtt ccagcaacgc
     1561 gtacgcacgg gccagtgcat cggcctccga cacgccgcac acgatcgggc cctcggcaat
     1621 cagcctgccc acgctgcagc gcgggtcgag cgagccgaag gggtcctgaa acacgatctg
     1681 cacgtagcgg cgatgtgcgc gcaatcgccg tcgcgaaagc tgcatccagt cgacatcgcg
     1741 cacgacgacg gacccgttgt cggcgtggac gaggcgcacg atgcagcgcg cgagcgtcga
     1801 cttgcccgat ccggattccc cgacgacgcc cagcgtttcg ccgtgccgga tcgcaagctc
     1861 gaccgacttc aaggcgcgca ccacgcgatc gcggccgccg aagctgccgc ttgcgcgata
     1921 cgtcttgtcg agggcgcgca cggtgagcgc gagcggcgcc gtttcgttcg ccgggcgcgt
     1981 catcggcgtc agcccgggaa tcgccgcgat cagcgcctgc gtatagggat gcgcgggtgc
     2041 gttcagcacc tgcggcgcat cgccgaactc gaccacccgg ccctgctgca tcacggcgac
     2101 gcggtcggcg atctcggcga ccacgccgaa atcgtgcgtg atgaacacca cgcccatgtg
     2161 ccgcgaccgc tgcacgtcgc ggatcagcgc caggatcccc gcctgcgtcg tcacgtcgag
     2221 ggcggtcgtc ggctcgtcgg cgatcagcac cgaaggctcg aggatcagcg ccatcgcgat
     2281 catcgcgcgc tggcgctgac cgccggagat ttcgtgcgga taggcgcgca tcaggtgctg
     2341 cgcatcggag aggcgcaccg attcgagcat cgccgcgatc cgctcggtgc gtgcgcgccg
     2401 tggaagcgcg gtgtgcaccg ccagcacctc gtcgatctgc gcaccgagcg tcttcaccgg
     2461 attcagcgcc gtcatcggct cctggaagat catcgagatg cggcggcagc ggatcgcacg
     2521 catgcgcgcc gcgtcggccc tcagcagatc ctcgccgtcg agcaggatgc ggccggcgct
     2581 tgcgcgcaaa tgcggcgccg gaagcaatcc catgatggcc cgtgccatca ccgattttcc
     2641 cgatcccgat tcgccgacga cgcacaggat ctcgttacgc cgcaacgcaa gactcacgcc
     2701 ggtcacggct tgcggacggt cggcccacga aggcaggtcc accgtcaggc gctcgatgtc
     2761 gaggatcgtc tcggacatgg gctacaggtg gcgaagccgc gggttcagcg catcgttgag
     2821 cccatcgccg acgaggttga tcgcgagcac ggtgacgaga atcgcgacgc cgggaatcgc
     2881 gcacagccac cacgcgcgct gcaggaagtc gcgggcggcg cccaccatga atccccagct
     2941 catcacgttg ggatccgaca ggccgagaaa cgacaacgcc gattcggtca ggatcgcggt
     3001 ggccacgagc agcgatccgg tgacgatgat cgacgacagg cagttgggca ggatatgcag
     3061 cgcgatcacg cgcaggtcgc tcatgcccat cgagacgcac gcctgcacga attcgcggcc
     3121 cgcgagcgag cggaactcgc cgcgcacgag ccgtgccatc ggcggccagg aaacgacggc
     3181 gatcgcgatg atcacgcttt cgatcgacgg gctcaggatc gcgacgacca ggatcgagaa
     3241 caggaacgac ggaatcgtct ggaagatctc ggtcagccgc atcagcgcct cgtcggcgac
     3301 gccgccgtaa tacccggcga tgcctcccag cagcacgccg agcagcgtcg cgaacaacgt
     3361 cgcgacgagc ccgatcacga gcgacgtgcg cgcaccgaag acgatcccga cgagctcgtc
     3421 gcggccgagc atgtcggtgc cgagcgcaaa acggccgaat ggcggctgga acggcggacc
     3481 ggccagctcg aacggaccag cgtgccgcaa cagatgcgga cccgcgatcg caacgacgac
     3541 gatggcgacg agaacgccga gcgcgaccag cgccgcgcga ttgcgcgcgt agcgttggca
     3601 gaaggcaaga acgtcggcct tcgcgctcat tgatcccccc acgcttgctg ctgcgcagct
     3661 gcgctgcccc ccgcgggggt ggaattcgcg ctttggggcg gcccggcagc gctcatcgca
     3721 ggcggatgcg cggatcgagc acggaataga tcacgtcgac gatgatgttg acggcgatca
     3781 cgaggcaggc ggcgacgaaa aagatgccga gcagcagatt gaggtcgcgc gcgaacagcg
     3841 cgcggaacgc gaggagcccg atgcccggcc agccgaacac cgtctcgacg acgaccgacc
     3901 cgccgagcag gttgccgacc tgcacgcccg ccatcgtcac gaccggcaga cacgcgttgc
     3961 gcagcacgtg ccgccaggta atcgtgcgct cggtcaagcc cttcgaccgc gcggtgacga
     4021 catagtccat gccgctctgc tcgagcatcg acgagcgcat cagccggacg tagagcgcca
     4081 tgtagaacag cgacagcgtg agcgcaggca gcacgagatg gcgcgcgacg tcacccgcct
     4141 tgcgccagcc ggtgtaaaac gccgcgatgt tggtcatgcc gctggtgggc agccacccca
     4201 gctggatcga gaacagcagg atgaacatga gcccgaccca gaacagcggc gtcgcgtacg
     4261 cgacgagggc gaaaatcgag atcgcgttat cgcgcagcga attgggacgc ccggcggcga
     4321 cgaggccgag caggatgccg acgccgatcg acagcacgat cgtcgtgccc atcagcagca
     4381 aggtcggacc cagtcgctgc agaatcaggt cgcgcaccgc cattccctcg cgaaacgagt
     4441 agccgagctc gagcgtgacc acgttcttca ggtaatcgag gtactgcacg tagagcggct
     4501 gatcgagccc gaactggtgc cggagatacg ccatgtattg cggcgtcgcc gacccggatt
     4561 cgccggccag gacgtcggcg gcgttgccgg gcgcaagatg caccagcagg aaattgagcg
     4621 tgacgatgac gaggatcgtc ggtaccgcct gcacgagccg gcgaccgatg tagcgcgcgc
     4681 gccctcgggc aacttccatg gaaggcgagc gcgcgatgtg ccggcgagcg actacgctgc
     4741 gagcgaagcc ttgttgaacg cggcgtacgt gccgagcggg ctgacggtca gatccttgaa
     4801 cttgctgtcg tacacgctca cgaagttgag ctccatcacc cacacgatcg gtgccgcttc
     4861 gacggtcagc ttctggaact ggtggtagag cgcgccgcgc ttcttcgggt cggtctcgac
     4921 ggcggcctcc ttcatcagcc gatcggtctc gggcgtgctc cagccggaat cgttgacgaa
     4981 cacggtgccc ttcttgatcg accacgacga ataaagacga tcgatgccga tcaccggatc
     5041 ggcgaggttc tggacccagt tcgacgtgag ctggaagtcg taatcggtgt agatgcgtcg
     5101 cagccacgtc gggacatcct cgtagcgcag cgtcgccttg accccgatct tggccagcgc
     5161 ctgcttgacg tattcgccgt agcgctgcca ttcgtcgccg tacggcgtga tgtcgtgcac
     5221 gatctcgaag cgggtgccgt cggtcttgcg cttgtaaccg gcctcgtcga gcatcttgtt
     5281 cgcgacgtcg gcgccattgg gcacgttgta gttcttgacg tccggcgtgt agatgccgct
     5341 cggtgcgaaa ttcgagctga tcggaccggt cgccggcttg ccgaagccga accagacgtt
     5401 gtcgatgacg aacttgcggt cgattgcaaa cgagatcgcc tggcgcaccc gcacgtcgtc
     5461 gaacggcttc acccgtgtat tgaaatcgag atggacgatc ggcgactgca tctcgtagcc
     5521 cttggtcgtc g
//
LOCUS       DASSVX010000121         2819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1154371, whole genome shotgun sequence.
ACCESSION   DASSVX010000121 DASSVX010000000
VERSION     DASSVX010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154371"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFO53_05350"
     CDS             complement(<1..249)
                     /locus_tag="VFO53_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875736.1"
                     /translation="MPSCLPFPLKLPLRYPLVLVAVVLVMAGCATHRPVPVEDRAVSR
                     NEPPRAGAPARVPPGTAPGEAAPVATYTVKRGDTLHQIA"
     gene            complement(269..961)
                     /locus_tag="VFO53_05355"
     CDS             complement(269..961)
                     /locus_tag="VFO53_05355"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET8875737.1"
                     /translation="MAAPGAGGRGTSGSGMTSDRTRARMVERLRTEGIRDEVVLSAMN
                     AIPRHIFVDEALAIRAYDDSPLPIGSGQTISQPWVVARMTELARNGRELDGVLEIGTG
                     CGYQTAVLARLAKTVYTVERIVALVNKARRHLQALKIRNVRLAHGDGSADLGEALAVD
                     AIIVAAGATHVPTALLRYLKPGGRMVLPLAKNDDGERGVQRLTVIEAAPAGIREQVFD
                     AVRFVPLLPGLA"
     gene            complement(984..1724)
                     /gene="surE"
                     /locus_tag="VFO53_05360"
     CDS             complement(984..1724)
                     /gene="surE"
                     /locus_tag="VFO53_05360"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET8875738.1"
                     /translation="MRILLSNDDGYFAPGLEQLAAALAPHAELTVVAPERDRSGASNS
                     LTLDRPLSVRRAPNGFLFVNGTPTDCVHLAVTGLLTELPDMVMSGINLGANMGDDTIY
                     SGTVAAATEGFLLGVPSVALSLASKTAAHFETAAAVALELLQRHKRRPAGPWLLNVNI
                     PDIERGEVQGYRITRLGRRHKAEDVVRMQSPRGETVYWVGAAGAAADAGEGTDFHAVG
                     NRYISVTPLQIDLTNREQMAGVGAWLGR"
     gene            complement(1811..>2819)
                     /locus_tag="VFO53_05365"
     CDS             complement(1811..>2819)
                     /locus_tag="VFO53_05365"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007467147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET8875739.1"
                     /translation="YLTSSWAIYRAERALVDATAAHDVRLRLFHGRGGTVGRGGGPSY
                     DAILAQPAGSVTGGLRVTEQGEIIASKYSDPELGRRNLEALVAATLEASLHDAERLGA
                     SAERYFAALDALSAHAYRAYRALVYETPEFVPYFRASTPIAEIAELNIGSRPASRSAS
                     SRIEDLRAIPWVFSWGQCRLMLPGWYGFGSAVEAWLADAPERLALLTEMHERWPFFRA
                     TLSNLGMVLAKTDLAIASRYAELVPDASVRDAIFARIRDEHALALRHFLAITRQSALL
                     EDNPTLARSIRNRFPYLDPLNHLQIELLRRYRAGATDVRTRRAIHLTINGLAAGLRNS
                     G"
BASE COUNT          431 a          950 c         1003 g          435 t
ORIGIN      
        1 cgcgatctgg tgcagcgtgt cgccgcgttt caccgtgtac gtcgcgacag gcgccgcttc
       61 accgggcgcg gtacccggcg gcacgcgcgc gggcgccccg gcgcgcggcg gctcgttgcg
      121 ggagaccgcg cgatcctcga cgggcaccgg ccgatgcgtg gcgcagccgg ccatgacgag
      181 cacgactgcg acgaggacga gcgggtatcg caatggcagc ttcagcggga acggcagaca
      241 ggatggcatc ggggcgaacg cagaactttc atgcaagccc cggcagcaga ggcacgaatc
      301 ttaccgcatc gaacacctgc tcacggatgc cggcgggcgc ggcttcgatg accgtgagcc
      361 gctgcacgcc gcgctcgccg tcgtcgttct ttgcaagcgg cagcaccatg cgcccgccgg
      421 gcttgaggta gcgcagcaac gccgtgggca cgtgcgtggc gccggcggcg acgatgatcg
      481 cgtcgacggc gagcgcctcg ccgagatcgg cgctgccgtc gccgtgcgcg aggcgcacgt
      541 tgcggatctt gagcgcctgc agatgccggc gcgccttgtt gacgagcgcg acgatgcgct
      601 cgaccgtgta gacggttttg gcgagccgcg cgagcacggc ggtctggtag ccgcaaccgg
      661 tgccgatctc gagcacgccg tcgagctcgc ggccgttacg cgcaagctcg gtcattcgcg
      721 cgacgaccca cggctgcgag atcgtctggc cgctgccgat cggcaacggc gaatcgtcgt
      781 aggcgcggat ggcgagcgct tcgtcgacga agatgtgccg cggaatggca ttcatcgccg
      841 aaagcaccac ttcgtcgcga atgccctcgg tgcgcagccg ctcgaccatg cgcgcacgcg
      901 tgcggtcgga cgtcatgccg ctgccgctcg ttccccgtcc ccctgcgccg ggcgccgcca
      961 tcaccgctcg tcctgttcgt tcttcaacgg ccgagccacg cgcccacgcc ggccatctgt
     1021 tcgcgattgg tgagatcgat ctgcagcggt gtcacggaga tgtagcggtt cccgacggcg
     1081 tggaaatcgg tgccttcacc ggcatcggcc gccgcgcccg ccgcgccaac ccagtacacg
     1141 gtttcgccgc gcgggctttg catgcgcacg acgtcctcgg ccttgtgccg ccggccgagg
     1201 cgcgtgatgc ggtatccctg cacttcaccg cgctcgatgt cggggatgtt cacgttcaga
     1261 agccagggac cggccggccg tcgcttgtgc cgctgcagca attcgagtgc cacggcggcg
     1321 gcggtctcga aatgcgcggc ggtcttcgac gcgagcgaca gcgcgaccga cggcacgccg
     1381 agcaggaagc cctcggtcgc ggcggcaacg gtgcccgagt agatcgtgtc gtcccccatg
     1441 ttggcaccga gattgatgcc ggacatcacc atgtcgggca attcggtcag caatccggtc
     1501 accgcgaggt gaacgcaatc ggtgggcgtc ccgttgacga acaggaagcc gttcggtgcc
     1561 cggcgcaccg acagcgggcg gtcgagcgtg agcgaattgg atgcgccgct gcggtcgcgc
     1621 tcgggtgcca cgacggtgag ctcggcgtgc ggcgcgagcg cggcggcaag ctgttcgagc
     1681 cccggcgcga aatagccgtc gtcgttggaa agtaggatcc gcatcggggc ggtacgggct
     1741 tgatgtttca tgcgacgagc gtcgtttgcc gcgcaccgcg atgggcgcga attataccgc
     1801 gcgctcgcgc ttacccgctg ttgcgcaatc ccgccgcaag cccgttgatc gtcaggtgga
     1861 tcgcacgccg ggtgcgcaca tcggtggcac cggcgcgata gcggcgcaac agctcgatct
     1921 gcaggtgatt gagcgggtcc aggtagggaa agcggttgcg gatgctgcgc gcgagcgtcg
     1981 ggttgtcttc cagcagcgcc gattgacgcg tgatcgcgag gaaatggcgc agcgccagcg
     2041 cgtgctcgtc gcgaatgcgc gcgaatatcg cgtcgcgcac gctcgcgtcg gggacgagct
     2101 ccgcgtagcg cgacgcaatc gcgagatccg tcttcgcgag caccatgccg aggttcgaca
     2161 gcgtcgcgcg gaaaaacggc cagcgctcgt gcatctcggt gagtagcgcg agccgctccg
     2221 gtgcgtcggc gagccaggct tcgaccgccg agccgaagcc gtaccacccg ggcagcatca
     2281 ggcggcactg gccccacgag aagacccacg gaatcgcgcg cagatcctcg attcgcgacg
     2341 atgcgctgcg cgacgccgga cggctgccga tattgagctc ggcgatctcc gcgatcggcg
     2401 tcgacgcgcg aaagtacggg acgaattccg gcgtttcgta gacgagcgct cgatacgcgc
     2461 ggtaggcgtg cgccgacaat gcgtcgagtg ccgcgaaata gcgctcggcc gatgcgccga
     2521 gccgctcggc atcgtgcagg ctcgcttcga gcgtcgccgc gacgagcgct tcgagattgc
     2581 gccggccgag ctcgggatcc gaatacttgc tcgcgatgat ctcgccctgc tcggtgactc
     2641 ggagcccgcc cgtgacgctg ccggccggct gcgccaggat cgcgtcgtag ctcggtccgc
     2701 cgccgcgccc gacggtgccg ccgcggccgt ggaagagccg cagccgcacg tcgtgcgccg
     2761 cggtggcatc gacgagcgcg cgttccgccc ggtagatcgc ccagctcgac gtcaggtag
//
LOCUS       DASSVX010000122         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1337682, whole genome shotgun sequence.
ACCESSION   DASSVX010000122 DASSVX010000000
VERSION     DASSVX010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337682"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..75
                     /gene="rplT"
                     /locus_tag="VFO53_05370"
     CDS             <1..75
                     /gene="rplT"
                     /locus_tag="VFO53_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379852.1"
                     /note="binds directly to 23S ribosomal RNA prior to in
                     vitro assembly of the 50S ribosomal subunit; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET8875740.1"
                     /translation="LADLAVHDKAAFTKIAEQAKASHA"
     gene            137..1123
                     /gene="pheS"
                     /locus_tag="VFO53_05375"
     CDS             137..1123
                     /gene="pheS"
                     /locus_tag="VFO53_05375"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET8875741.1"
                     /translation="MDDIDRTVESALADFQACADPAALENVKAKYLGKAGALTARLKS
                     LGALPPGERAAAGARINAAKATLEEALVARRQSIADLKLATQLSADALDVSLPGRGAS
                     IGGLHPLTRTLERIETLFRSLGFDVADGPEIEDDFHNFTALNTPENHPARSMHDTFYV
                     EGGLVLRTHTSPIQVRYMETHAPPIRIIAPGRVYRVDSDATHSPMFHQVEGLWIGDDV
                     SFADLKAVLSAFLRNFFERDDLTLRFRPSFFPFTEPSAEIDMRFGDGGWLEIAGAGQV
                     HPNVLRAVSIDPERWQGFAFGIGPDRLAMLRYGVDDLRLFYENDLRFLAQFR"
     gene            1224..>2830
                     /gene="pheT"
                     /locus_tag="VFO53_05380"
     CDS             1224..>2830
                     /gene="pheT"
                     /locus_tag="VFO53_05380"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET8875742.1"
                     /translation="MKFSERWLRTLVDPPIDTAALCDKLTMAGFEVEDVARAAPPFTN
                     VVVGTITHVALHPGADRLRVCTVDTGRDRALQVVCGAPNAAVGLRVACALDGATLPGE
                     QSITRTTMRGVESQGMLCSARELGIADDASGLLVLPSDAKPGEDLRTALALDDALITI
                     KLTPNRPDCLSIVGIAREVSAITRAPLTFPGESRAPVSAGATRGVRIEDAAACPRFAA
                     RIIDGIDAKAPTPEWMKERIERSGLRSISAVVDITNYVMLELGQPLHAYDNRLLDGDI
                     VVRFAREGETLTLLNGQTLPLEPDLLLVCDGVKPLGLAGIMGGEHSGIADDTTSVYLE
                     GAFWNPAVIQGKMRRLGFVSDAGYRFERGVDFELGPRAVERATQLVLDICGGRAGPLT
                     DARGALPSRPPVRLRTERAARILGVTLSSDAIADVFTRLRLAFVREGGDFVVTPPSYR
                     FDLAIEEDLIEEVARIHGYDAIPDAPRAHVQAMLPSPESHRGIASIKRRLVLRDWQEV
                     VTFSFVSSDDERALDPDVHPLAVLNPIA"
BASE COUNT          441 a          982 c          925 g          482 t
ORIGIN      
        1 ctcgccgacc tcgccgtgca cgacaaggcc gcgtttacga agatcgcgga acaggcgaaa
       61 gcttcccacg cctgagccga gcgatcgaac tccgggggag gcgcgaaacg cctccctttt
      121 ttgtttgatg agcgacatgg atgacatcga ccgcaccgtc gaaagcgcac tggccgattt
      181 tcaggcctgc gccgatccgg ccgcgctcga gaacgtcaag gcgaagtatc tcggcaaggc
      241 cggcgcactc accgcacggt tgaagtcgct cggcgcgctg cctcccggcg agcgcgcggc
      301 ggccggcgcg cgcatcaacg cggccaaggc aacgctcgag gaagcgctgg tcgcgcggcg
      361 tcaatcgatt gccgacctga agctcgcgac acagctttcg gccgacgcgc tcgacgtctc
      421 gctgccggga cgcggcgctt cgatcggcgg actgcacccg ctcacgcgca cgctcgagcg
      481 catcgagacg ctctttcgct cgctcgggtt cgacgtcgcc gacggacccg agatcgagga
      541 cgacttccac aacttcaccg cgctcaatac gccggagaac catcccgcgc gctcgatgca
      601 cgacacgttc tatgtcgagg gcggcctcgt tttgcgcacg catacgtcgc cgatccaggt
      661 gcgctacatg gagacgcatg cgccgccgat ccggatcatc gcgcccggac gcgtctatcg
      721 cgtcgacagt gacgcgacac actcgccgat gttccatcag gtggagggcc tgtggatcgg
      781 tgacgacgtg tcgttcgcgg atctgaaggc ggtgctcagc gcgttcctgc gcaacttctt
      841 cgagcgcgac gacctgacgc tgcgcttccg gccgtcgttc tttccgttca ccgagccgtc
      901 ggccgaaatc gacatgcgct tcggcgacgg cggctggctc gagatcgcgg gcgccggcca
      961 ggtgcatccg aacgtgctgc gcgcggtgag catcgatccc gagcgctggc agggattcgc
     1021 gttcggcatc gggccagatc gccttgcgat gctgcgctac ggcgtcgacg acctgcgcct
     1081 gttctacgag aacgacctgc gcttcctcgc gcaattcaga tgacgctcgc tgccgcctgg
     1141 gatgaaacat ccccccacgc tcgctccgct cgctgccccc caaggggctg gtcagtcgct
     1201 cggggcggcc cttcccgact gacatgaaat tctccgaacg ctggctgcgc acgctcgtcg
     1261 atccgccgat cgacacggcg gcgttgtgcg acaagctgac gatggcgggc ttcgaggtcg
     1321 aggatgttgc gcgcgcggct cccccgttca cgaacgtcgt cgtcggcacg atcacgcatg
     1381 tcgccctgca tcccggcgcc gaccggctgc gcgtgtgtac cgtggacacc ggacgcgatc
     1441 gcgcgttgca ggtcgtctgc ggtgcgccga acgccgcggt cggcttgcgc gtggcgtgcg
     1501 cgctcgacgg cgcgacgctg cccggcgagc aatcgatcac gcgcacgacg atgcgcggcg
     1561 tggaatcgca gggcatgctg tgctcggcac gcgagctcgg catcgccgac gatgcgtcgg
     1621 gactgctcgt gcttccatcc gatgcaaagc ccggcgagga tctgcgcacg gcactcgcgc
     1681 tcgacgacgc gctgataacg atcaagctga cgccgaaccg gcccgactgc ctgtcgatcg
     1741 tcggcatcgc gcgcgaggtt tcggcgatca cgcgcgcgcc gttgacgttc cccggcgaat
     1801 cgcgcgcacc ggtgtcggca ggtgcgacgc gtggggtgcg catcgaggat gccgccgcct
     1861 gtccgcgctt cgcagcgcgc atcatcgacg gcatcgacgc gaaggcgccg acgcccgaat
     1921 ggatgaagga gcgcatcgag cgctcgggat tgcgctcgat ctctgcggtg gtcgacatca
     1981 cgaactacgt gatgctcgag ctcgggcagc ccctgcacgc ttatgacaat cgactcctcg
     2041 acggcgacat cgtcgtgcgc ttcgcacgcg aaggtgaaac gctgacactg ctgaacggcc
     2101 agacgctgcc gctcgagccc gacctcctgc tcgtctgcga cggcgtgaag ccgctcggtc
     2161 ttgccggcat catggggggc gagcattccg gcatcgccga tgacacgacg agcgtatatc
     2221 tcgaaggcgc gttctggaac ccggcagtga tccagggcaa gatgcgccgc ctcggcttcg
     2281 tgagcgatgc cggctatcgc ttcgaacgcg gcgtcgattt cgagctcggg ccgcgcgccg
     2341 ttgagcgcgc cacgcaactg gtcctcgaca tctgcggtgg ccgtgccggc ccgctcaccg
     2401 acgcaagagg cgcccttccc agccgcccgc cggttcggct tcgcaccgaa cgtgcagcgc
     2461 gcattctcgg tgtgacgctg tcgtccgatg cgatcgccga cgtcttcacg cgcctgcgcc
     2521 tcgcgttcgt tcgcgaaggc ggcgatttcg tcgtcacgcc gccgtcgtat cgcttcgacc
     2581 tcgcgatcga ggaggacctg atcgaggaag tcgcgcgcat tcacggctac gacgcaatcc
     2641 ccgacgcgcc gcgcgcgcat gtgcaggcga tgctgccgtc gcccgaatcc catcgcggca
     2701 ttgcatcgat caagcggcgc ctcgtgctgc gcgactggca ggaggtggtc acgttcagct
     2761 tcgtttcgtc ggatgacgag cgcgccctcg atcccgatgt gcatccgctg gccgtgctga
     2821 atccgatcgc
//
LOCUS       DASSVX010000123         3857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_887844, whole genome shotgun sequence.
ACCESSION   DASSVX010000123 DASSVX010000000
VERSION     DASSVX010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887844"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1030
                     /locus_tag="VFO53_05385"
     CDS             17..1030
                     /locus_tag="VFO53_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HET8875743.1"
                     /translation="MIHGIDMQVADGEFIVIVGPSGCGKSTLLRMVAGLEAITSGEIA
                     IGDRVVNDLEPKDRDIAMVFQNYALYPHMSVFNNMAYGLRIRGLSKADIGERVQRAAD
                     ILELGPLLARTPRQLSGGQRQRVAMGRAIVREPAVFLFDEPLSNLDAKLRVQMRFEIQ
                     KLHRRLSTTSLYVTHDQVEAMTLAQRMIVMNAGRAEQIGSPMQVYENPQTMFVAGFIG
                     SPSMNFMRGKANGDGRIALEHGGEVRSRHVPAESGRALTVGIRPEHLIPSTEAGAALS
                     GPVEMVEQLGADALVHISRGGSTIIARVPHGTSPEVGATFHVTADPARVFAFDTDTGA
                     RLQ"
     gene            1032..1766
                     /gene="ugpQ"
                     /locus_tag="VFO53_05390"
     CDS             1032..1766
                     /gene="ugpQ"
                     /locus_tag="VFO53_05390"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET8875744.1"
                     /translation="MNPWPYPRLCAHRGAGKLAPENTLAAFRLGYAHGYRMAEFDVKL
                     SADGVPFLLHDDTLDRTTNAQGRADAHTWRELSQLDAGSWHSSAYAGEPLCTLASVAR
                     WSLAHDVTVNIEIKPTRGRERETGAAVAFDAATLWADARVPPLLSSFSDVALDAARDA
                     MPALPRAWLADRLPDDWRARAARADCIAVDLEHTLLNRALAEEIHAADMRVLCWTVND
                     PILVERLFDLGADTVITDAVDILAAR"
     gene            1901..3805
                     /locus_tag="VFO53_05395"
     CDS             1901..3805
                     /locus_tag="VFO53_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8875745.1"
                     /translation="MSLVRLDVSQRNIASLLQIFDRAQLPSGASFVLVLHADDRDAAD
                     LRARIDGLARVPVIHVEQTTHLEGDRVYVVSASKPFTLSGDTIELAEHDEAATRLRRS
                     EQRYRSIMDSVRDYAILTTDLRGIIDGWNTGAEKLFGYSRAEILGRSVDILFTPEDRA
                     AGAPRIEMATARAKGRAEDERFHLRKDGTRFYCSGVLSPMIDGEIYGYVKIARDLTKK
                     QAEATEHERQFLHVKSEHLELQNVSQLKDRFLATLSHELRNPLNLILMQSEILSRSLD
                     ARAQPMLARAADIIAQAVKVQARLIDDLLDVSRINTGKLALEQQLLPLPAIVSESITA
                     LKNEMERKEITLDLAVSAAPLIVHGDPMRVRQIAWNLVSNAIKFTPAGGRIAVRVKRE
                     GDEARLDVEDNGQGIAREFMPHVFELFSQKDSGIARRYSGMGIGLALVRQLVDLHNGR
                     VEAYSAGEGQGARFSVWLPLDNAPDVAPHSLAADAMPGNPGPLPGIGNAREREAARRL
                     DGLRVLVVDDDAASAEALRELLHDEGARVRAANGAPGALALAGQSDFDVVISDVAMPD
                     MDGHAFLRKLREDPRYANVPAIACTGYGSDADVSRAEVAGYVAYLVKPLDIEGVIGAI
                     RTAIAGRKGR"
BASE COUNT          648 a         1289 c         1301 g          619 t
ORIGIN      
        1 ctacggcacg ctcgaggtga tccacggcat cgacatgcag gtcgccgacg gcgagttcat
       61 cgtcatcgtc gggccgtcgg gctgcggcaa gtcgacgctg ctgcgcatgg tggcgggact
      121 cgaagcgatc acgagcggcg aaatcgccat cggcgaccgc gtggtcaacg acctcgagcc
      181 gaaggaccgc gacatcgcga tggtgttcca gaactacgca ctctatccgc acatgagcgt
      241 gttcaacaac atggcgtacg gactgcgcat ccgcggcttg tcgaaggccg acatcggcga
      301 gcgcgtgcag cgcgctgcgg acatcctcga gctcgggccg ctgctcgcgc gcacgccgcg
      361 ccagctctcc ggcggccagc ggcagcgcgt cgcgatgggc cgcgccatcg tgcgcgagcc
      421 cgcggtgttc ctgttcgacg agccgttgtc gaacctcgat gcgaagcttc gcgtgcagat
      481 gcgcttcgag atccagaagc tgcaccggcg attgtcgacg acgagccttt acgtcacgca
      541 cgaccaggtc gaggcgatga cgctcgcgca gcggatgatc gtgatgaacg ccgggcgcgc
      601 cgaacagatc ggctcgccga tgcaggtgta cgagaacccg cagacgatgt tcgtcgccgg
      661 cttcatcggc tcgccgtcga tgaacttcat gcgcggcaag gcgaacggcg atggccgtat
      721 cgcgctcgag catggcggcg aagtgcgcag ccgccacgtg ccggccgaga gcggacgggc
      781 gctcaccgtc ggcatccggc ccgagcatct catcccttcc accgaggcgg gcgccgccct
      841 gtcggggccg gtggagatgg tcgagcagct cggcgccgac gcgctcgttc acatcagccg
      901 tggcggcagc accatcattg cgcgcgttcc gcacggcacg tcgccggagg tcggtgcaac
      961 ctttcacgtg acggccgacc cggcgcgggt gtttgcgttc gacaccgaca cgggcgcgcg
     1021 gctgcagtga gatgaacccc tggccctacc cgcgtctatg cgcgcaccgc ggcgcgggca
     1081 agctcgcgcc ggagaacacg ctcgccgcgt tccggctcgg ttacgcgcac ggctaccgga
     1141 tggccgagtt cgacgtcaag ctctcggccg acggcgttcc gttcctgctg cacgacgaca
     1201 cgctcgatcg cacgaccaac gcgcaaggcc gcgccgatgc gcacacctgg cgcgagctgt
     1261 cgcaactcga cgccggaagc tggcattcat cggcgtacgc cggcgagccg ctgtgcacgc
     1321 tcgcgagcgt cgcgcgctgg agtctcgcgc acgacgtcac cgtgaacatc gagatcaagc
     1381 ccactcgcgg ccgcgagcgc gaaacgggcg ccgcggttgc cttcgatgcc gcgacgctgt
     1441 gggcagacgc gcgagtgccg ccgctgcttt catcgttctc cgacgtggcg ctcgacgcgg
     1501 cgcgcgatgc catgccggcg cttccgcggg cgtggttggc cgaccggttg cccgacgact
     1561 ggcgtgcgcg cgcggcgcgc gccgactgta tcgccgtcga tctcgagcac acgctgctga
     1621 accgcgcgct ggccgaggaa atccacgcgg ccgacatgcg cgtgctctgc tggaccgtga
     1681 acgacccgat tctggttgaa cgtttgttcg atctcggcgc cgacaccgtg atcacagacg
     1741 cggtcgacat cctcgccgcg cgataacgct tggggcaggc tgcatttgtg tggtgtggca
     1801 atctggcgca cactgcaacg acttctcgtg cgcctcccgc gctgtttcga tgagccaatc
     1861 ttttcaatct aaagaggggc gtccgggcgc gcaatccggc atgtcccttg tccggctgga
     1921 tgtctcccag cgcaatatcg cttcgcttct gcaaatcttc gaccgggcgc agttgccgag
     1981 cggcgcttcg ttcgttctcg tcctgcacgc cgacgatcga gacgcggcag atctgcgtgc
     2041 gcggatcgac gggcttgcgc gcgttccggt tatccatgtc gaacaaacga cgcacctcga
     2101 aggcgatcgc gtgtacgtcg tgtcggcgtc gaagccgttc acgctttcgg gggatacgat
     2161 cgagctcgca gagcacgacg aagctgcgac gcgcctgcgg cgcagcgagc agcgctaccg
     2221 gtccatcatg gacagcgtgc gcgattacgc gatcctgacg accgatcttc gcggcatcat
     2281 cgacggctgg aacaccggcg ccgagaagct cttcggctac agccgggcgg aaattctcgg
     2341 ccggtcagtc gatatcctgt tcacgcccga ggaccgcgcg gccggcgcgc cccggatcga
     2401 aatggcgacc gcacgcgcga agggccgtgc ggaggacgag cgcttccatc tgcgcaagga
     2461 cggcacgcgc ttctattgca gcggcgtgct ctcgccgatg atcgacggcg agatctatgg
     2521 ttatgtgaag atcgcgcgcg atctgacaaa gaagcaggcg gaggcaaccg aacacgaacg
     2581 gcaatttctg catgtcaagt ccgagcacct cgagctgcag aacgtgagtc agctcaagga
     2641 ccgcttcctt gccacgctat cgcacgagct gcgcaacccg ctcaacctga ttctcatgca
     2701 gtcggagatc ctgagccgat cgctggacgc gagggcgcag cccatgctgg cccgcgcggc
     2761 cgacatcatc gcgcaggcgg tgaaggtgca ggcgcgtcta atcgacgatc tgctcgacgt
     2821 gtcgcgcatc aacaccggga agcttgcgct cgaacaacag ctgcttccac tgccggcgat
     2881 cgtctccgaa tcgatcaccg cgctgaagaa cgagatggag cgcaaggaga tcacgctcga
     2941 cctcgcggtc agcgccgcgc cgctgatcgt ccatggggat ccgatgcgcg ttcggcagat
     3001 cgcctggaac ctcgtgagca atgcgatcaa gttcacgcct gccggtgggc gcatcgccgt
     3061 gcgggtcaag cgcgaaggcg atgaggcgcg cctcgacgtc gaagacaacg gacagggcat
     3121 tgcgcgggag ttcatgccgc acgtcttcga gctgttcagc cagaaggatt cgggcatcgc
     3181 gcgccgttac agcggcatgg ggatcggcct cgccctggtg cgtcagctcg tggatctcca
     3241 caacgggcgc gtcgaagcgt attccgccgg cgagggccaa ggcgcccgct tcagcgtctg
     3301 gcttccgctg gacaacgcgc cggatgtcgc gccgcattcg cttgcagcgg acgcgatgcc
     3361 gggcaacccg ggaccgctgc ccggcatcgg taatgcgcgc gagagagagg cggcacggcg
     3421 gctcgatggc ttgcgggtgc tcgtggtcga cgacgacgcg gccagcgccg aagcgctacg
     3481 cgagttgctc cacgacgaag gggcccgtgt aagagcggct aacggcgcgc ccggcgcgct
     3541 cgcgctcgcc gggcagagcg atttcgatgt cgtgatttcg gacgtcgcga tgcccgacat
     3601 ggacgggcac gcgttcttgc gcaaactgcg cgaagacccc cggtacgcga acgtgcccgc
     3661 gatcgcctgc accggttacg gcagcgacgc cgacgtgagc cgggcggaag tcgccgggta
     3721 cgttgcctac ctcgtgaagc cgctcgacat cgagggcgtc atcggcgcca ttcggacggc
     3781 cattgcgggc cgcaagggcc gctgactgac gctcgtcgcg cagcactgcg cgctgcacgg
     3841 cagctcaggc gggcgcc
//
LOCUS       DASSVX010000124        12673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_71074, whole genome shotgun sequence.
ACCESSION   DASSVX010000124 DASSVX010000000
VERSION     DASSVX010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12673
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71074"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFO53_05400"
     CDS             complement(<1..518)
                     /locus_tag="VFO53_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET8875746.1"
                     /translation="MLPLPAFAFVDLETTGTRPGADRITEIGIVRVDDDGAGLRVTEW
                     SSLVDPEVPIPPVIQALTGITDAMVASAPSFSRIANDVRDLLAGCVFVAHNARFDYGF
                     LKHAFARLERAFSARVLCTVRLSRRLFPEADGHGLDALIARHAIAVTERHRALGDARA
                     IWSFVEDLYRRQS"
     gene            590..1165
                     /locus_tag="VFO53_05405"
     CDS             590..1165
                     /locus_tag="VFO53_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HET8875747.1"
                     /translation="MSNDRSYLGTLQDYYARHRALPSYASIGSLLGLRSKSSVAALVA
                     RMKLAGYLESTPDRRLAPTKRFFARPLATGFVRAGLPEPIADDEADAVTLDDYLIERP
                     SQTVLVRVRGDSMIDAGIFEGDLVVVEKRAAAQKGDIVVAIVDNQFTLKRLDLENGRF
                     VLKPENKGYAVIRPEGELEIFGVMIGLVRKL"
     gene            complement(1171..3264)
                     /locus_tag="VFO53_05410"
     CDS             complement(1171..3264)
                     /locus_tag="VFO53_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HET8875748.1"
                     /translation="MRGMPLDSSGPANRRIVRAACPHDCPDTCAMLVTVEDGRAVDIR
                     GASDHPPTGGTLCTKVARYLERTYSPDRLRYPMRRVGAKGEGRFARISWDEALDEITT
                     RFKAIAASADGPQAILPYSYAGTMGLLQGSSMDRRFFHRLGASLLDRTICASAGKAGW
                     AAVIGASMGMDLEEYAGSRLILIWGSNPIASNLHFWTRAQEAKRHGAKLIAIDPYRSA
                     TAEKCHEHIALLPGTDAALAFGIMHVLIDEALVDRDYVDRYTVGYDALAARVREWPPA
                     RVAATCGIRAEQVVSLAHDYGSIKPAAIRLNYGMQRVHGGGNAVRAVACLPALVGAWR
                     DPAGGALLSSSGTFPVDKSALERPDLIQGAPRTINMSTIGDALTRVDDPPIRAIYVYN
                     SNPVAVAPESSRVVAGFSRADLFCVVHEIFRTDTADYADILLPATTQLEQTDIHSSYG
                     HLYAVANNPAIAPIAEALPNTEVFRRLAARMGFTEPCFRDSDDEMAREAYRAQDLHGI
                     GIDWEALKAAGFKRLNVPRPYAPFAEGRFPTPSGKCEFWSQTLAGQGDDPLPSYLPPR
                     ESAASNPELAARYPLGFISPPARNFLNSSFANLPSFLAEEKTPHLDIHPDDASSRGIA
                     SGDRVRVFNDRGSLLVTARVTDRARAGVVVAPSIWWRRLAPGGENANAVTSQALTDLG
                     RAPTFYDCLVEVRPA"
     gene            3497..3835
                     /locus_tag="VFO53_05415"
     CDS             3497..3835
                     /locus_tag="VFO53_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET8875749.1"
                     /translation="MVNYTHYDYLELAPGASAQRVEAAYCALLERFQYGHTDAGQDLS
                     GLVRMIHAAYAVLSDPAQRRAYDERLTIEAQAADAELQSQLDAAAITGFTRVQQVPDA
                     LLATIPELAA"
     gene            4166..4522
                     /locus_tag="VFO53_05420"
     CDS             4166..4522
                     /locus_tag="VFO53_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875750.1"
                     /translation="MEKCVGCQFYDRNDDHVEDKGVRWGKCRRGVPIVHPVSAKAYMV
                     EGIWPHVRDDDWCGEWSAIKRKPDTSMPDARNLMAQPSTPSPIRTAGLPGGSLMTPVA
                     PVGEGAAALNGRFGSD"
     gene            4685..7360
                     /locus_tag="VFO53_05425"
     CDS             4685..7360
                     /locus_tag="VFO53_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET8875751.1"
                     /translation="MSNLLEALAAGATVVTPNNRLARDVAARFDTAQRASGRHAWSAA
                     NALPFTLWLDRLWRSALAAGALRAPPVLLDASATRALWHAIVAQHRHDWLNAHGAARH
                     AADAWTTFHRYRAPGESIADIASNALHDDPIVFGRWAQRYQAQLRALNAIDDAQLPDV
                     LTAHETASSLAGCPPVNLHGFIALTPQQRRLVAALRAAGATIDESPATGSAATRCQRV
                     GLATPRDELVHAFGHARERLLRDPDARVAIVVADLDERRSEVLALAEETLCAEHLLAI
                     TPDAVRPYGVSLGEPLAGVPLVVAALDLIALAVGRLDATTATSLVRTPFLPDAATQWT
                     RRAQAEQHWLELGLREVAWPDVLAALRTVDPILHRRFAVIPPPTHALRAPRDWARAWS
                     DWLAALGWPGSATLTSGEWQAREAWSAALARFASIGSVTGTLSPVAALDALRALLAET
                     LFQPEIAPAPVQILGVLEAAGLTFDCAWLAGFDAQRWPGTAVSNPLLPLRWQQARRVP
                     RAHPEAVIEQACAITSALQRIAPEIVVSHAMTIDDAPAALSPLFAHWPPMADALLPAA
                     SRITGLVPAAPMESVAEPSAPPLVDGETIRGGAGLFDSQSACAFQAFARYRLDARSWP
                     SCPDGLSPAERGEMLHATLKAFWDATGDQSALAALDAHALALRIADAVKAGKARIGSP
                     RWRTLAPAVAIAEASRLAATLHAWIDEGERTRPPFRVRGHEQRVRCSIEGIALSIRVD
                     RIDELPGGGLAVIDYKSGNVVSPARWFMERPEGIQLAIYAAAVEDATREPVRALAYAQ
                     VKAGNIEVAGLAEDNAQWPALASVDSTRLALADWADARAELAARVHALASDIRNGIAD
                     VAPRTLAICGYCGLQPLCRVQRLDDGASAADTRDE"
     gene            7353..10754
                     /locus_tag="VFO53_05430"
     CDS             7353..10754
                     /locus_tag="VFO53_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET8875752.1"
                     /translation="MNDVAGLAVRDDAARAQALDITRSWLVQAPAGSGKTSLLIQRFL
                     ALLARVERPERIVAMTFTRKAAAEMRARIVDALRNAALAPDEARIGAHETLTRALARR
                     ALDQDRVMGWRLLEQPARLRIVTIDALAAALARQVPLAAGLGALPGFVDDARALHVDA
                     ARAALAEAAPLDRHWQAFLQWQDNDAGAATRLIAQMLAARERWPLPMLDGDPAVLRAE
                     VEAVLELEARAAVAIVHERLPASLAAALPRLAQMALDGLHELGIDCAHAGALAALCRD
                     GRLPDPQTRETWCGLAQWLLTQEGTFFRVVTKRHGFPRGAPQTQERARRKAEFVRWLE
                     KAAQVPGLADAWHRLRALPPARFSDDAWAFVAAAMRVLPRAVAALDGVFRARGRADFA
                     EATLRALTALGTPDDPSDLLLAIDYRLSHLLIDEFQDTSSAQLALIGKLTSGWDAGDG
                     RTLFAVGDPMQSIYRFRQADVALFLDAQAQARIGDVPVGVVELSRNFRSQPAIVDFVN
                     DVFRAVLPALSDPAQAEAAYRPAYADPERGVDIAPTLDLVGSRDAEAGVVVQRIRDAQ
                     AAGVADIAILVRARSHAQALLPALRRAGIDYSAIDLEGLHDRLATRDLLSLARALAQP
                     ADRLAWLSVLRAPWCGLTLADLHVVANAGASTIAAALEAPDAIDRLSSDGRARVARFR
                     QCVAPALASRGQASFTARVKSAWVALGGPACAASPLDRDGADRVFALLAEHERGADLP
                     DFETLRDTAQRLFADAGKAASAAVQVMTLHKAKGLQFGAVILPGLDLRAGRADSPLLR
                     WKVRRHHGQRTLLIAPMRARMGARSEPDPVYAWLGEIDAAEEAAELGRLLYVGATRAK
                     RRLHLVAVASVDSKLEACEETRSFKRPARGSALERLWDALAAYVPRVAPSPAPVRGEQ
                     SPPDHGVSLIRLPLGWQLPALPPPLPVAHQRATMPDAPTFDWADATAAAIGTVAHRLL
                     AQVAAEGVAAWGARRLVLEQPRILAELGSEGVEPDLAVDAATRVTTIVARTLRDPRGR
                     WLFDPAHVDAHSEWALAGDDDGRIVHVVLDRSFVAAGRRYVIDFKTGAHLGGDPATFL
                     AREFERYRPQLQRYARIVRAIDARPVRVALYHPLVEGGWQEHELD"
     gene            10822..11742
                     /locus_tag="VFO53_05435"
     CDS             10822..11742
                     /locus_tag="VFO53_05435"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET8875753.1"
                     /translation="MRVKRDLTSAPDGPVALTIGNFDGVHRGHQAMLARLIEAAEDLR
                     LPPAVLTFDPHPREFFSPSNAPPRLSTLRSKLALFRAYGVATTFVARFDARLASRTAD
                     EFVRGILESQLHTRWLLVGEDFRFGRGRTGDLAFLRSAAREFSVEAMDTIGVDGQRVS
                     STAVRNALAAGDLVLAAALLGRNYTLTGHVAHGDKIGRSLGFPTMNLPLRHPPAMTGI
                     FAVRVHGIGNMPRTGVASVGVRPTLRAGGRPIAEVFLFDFDQATYGRRITVEFLHKLR
                     DEARFPDLDALRQQMMRDVADARDHFAAKR"
     gene            11786..>12673
                     /locus_tag="VFO53_05440"
     CDS             11786..>12673
                     /locus_tag="VFO53_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET8875754.1"
                     /translation="MPDDARTDYKPTLNLPDTPFPMRGDLARREPAWVAAWRRNDVYA
                     AIRAASRGRPRFILHDGPPYANGDIHIGHAVNKILKDVIVKSRTLAGFDAPYVPGWDC
                     HGMPIEVQIEKTYGKELSTAETQRLCRAYAAEQIERQKKDFERLGVLADWDHPYTTMS
                     YANEADEIRALGKVLEKGYLYRGLKPVNWCFDCHSALAEAEVEYEDRVDTAIDVGFAL
                     DDPAERAKLAQAFGLSHAPDGPIYAMIWTTTPWTIPANQALSVHPDLEYALVATPRGH
                     LVLAKDLAGACLARYGLDGS"
BASE COUNT         1930 a         4408 c         4358 g         1977 t
ORIGIN      
        1 gattgccggc ggtacaggtc ctcgacgaac gaccaaatcg cgcgcgcatc gccgagcgcg
       61 cgatgacgct cggtcaccgc gattgcgtgg cgggcgatca gcgcgtcgag cccgtgtcca
      121 tccgcttcgg ggaatagccg ccgcgacaag cgcaccgtac aaagcacgcg tgccgagaaa
      181 gcgcgctcga gccgcgcgaa cgcatgcttg agaaagccat agtcgaagcg cgcgttgtgc
      241 gcgacgaaca cgcagcccgc cagcaggtcg cgcacgtcat tcgcaatcct cgaaaacgat
      301 ggggcgcttg ccaccatcgc atcggtgatg ccggtcagcg cctggatgac cggcgggatc
      361 ggcacttcgg ggtcgacgag cgagctccat tcggtgacgc ggagaccggc gccatcgtcg
      421 tcgacgcgca cgatgccgat ctcggtgatc cggtcggccc cggggcgcgt gccggtggtc
      481 tcgagatcga cgaacgcaaa ggcaggcaag ggcagcatcg ggggcacgga acgcgcgagg
      541 cgaacgaacg gccttgacac gccgaacgtt cgttcgctat tctagccgaa tgtcgaacga
      601 tcgttcctat ctcggcacgc tgcaggacta ctacgcgcgg catcgcgcgc tgccatcgta
      661 tgcttcgatc ggcagcctac ttgggttgcg gtcgaaatcg tcggtggcgg cactcgtcgc
      721 gcgaatgaag ctcgccggct atctcgaatc gacgcccgat cggcgtctcg cccccacgaa
      781 gcgctttttc gcgcgtcccc tcgccacggg cttcgtgcgc gccggcctgc ccgagcctat
      841 cgccgacgac gaagccgatg ccgtcacgct cgacgactac ctgatcgagc ggccctcgca
      901 aaccgtgctc gtgcgcgtac gcggcgattc gatgatcgac gccggcatct tcgaaggcga
      961 cctcgtcgtc gtcgaaaagc gcgcggccgc gcagaagggc gacatcgtcg tcgcgatcgt
     1021 cgacaaccag ttcacgctga agcggctcga cctcgaaaac ggccgtttcg tcctgaagcc
     1081 cgagaacaag ggttacgccg tcatccgtcc ggaaggcgag ctcgagatct tcggcgtgat
     1141 gatcggcctc gtgcgaaaac tctaggcggc ctacgcgggt cgaacttcga cgaggcaatc
     1201 gtagaacgtg ggcgcgcgac cgagatcggt cagcgcctgg ctcgtcaccg cattggcgtt
     1261 ttcgccgccc ggcgccagcc tgcgccacca gatcgacggt gccaccacca cgccggcacg
     1321 cgcccgatcg gtcacgcgcg cggtgaccag caggctgccg cggtcgttga atacgcgcac
     1381 ccgatcaccg cttgcaatgc cgcgcgacga cgcatcgtcg ggatggatgt cgagatgcgg
     1441 cgtcttctcc tcggcaagga acgacggcag gttcgcgaac gacgaattga ggaagttgcg
     1501 cgccggtggt gaaatgaagc ccagcggata gcgcgccgcg agctccgggt tcgacgcagc
     1561 cgattcgcgc ggcggcaggt acgagggtaa cgggtcgtcg ccttgcccgg ccagggtttg
     1621 cgaccagaat tcgcatttgc ccgacggcgt cggaaaccgg ccctcggcga acggcgcgta
     1681 cggacgcggc acgttcaacc gcttgaaacc cgccgccttc agcgcctccc agtcgatccc
     1741 gatgccgtgc agatcctgcg cacggtacgc ctcgcgcgcc atttcatcgt cgctgtcgcg
     1801 aaagcacggc tcggtgaatc ccatgcgcgc ggcgagacgg cgaaacacct cggtattggg
     1861 caacgcctcg gcgatcggcg cgatcgccgg attgttcgcg accgcataca ggtgtccgta
     1921 ggagctgtga atgtcggtct gctcgagctg cgtcgtggcc ggcagcagaa tgtcggcgta
     1981 atcggccgtg tcggtgcgga agatctcgtg cacgacacag aacaggtcgg cgcgtgaaaa
     2041 gccggcgacg acacgcgacg attcgggcgc aacggcgacc ggattcgagt tgtagacgta
     2101 gatcgcgcga atcggcggat cgtcgacgcg cgtcaatgca tcgccgatcg tcgacatgtt
     2161 gatcgtccgc ggcgcgccct ggatcagatc gggacgctcg agcgccgatt tgtcgacggg
     2221 aaacgtgccc gacgacgaca gcagcgcgcc gcccgcgggg tcgcgccatg ctccgacgag
     2281 cgctggcagg cacgcgaccg cacgcacggc gttgcccccg ccgtgcacgc gctgcattcc
     2341 atagttgagg cgtatcgcgg ccggcttgat cgagccgtag tcgtgcgcga ggctcacgac
     2401 ctgttcggcg cggattccgc aggtggccgc cacgcgggcg ggcggccact cgcgcacgcg
     2461 cgccgccagc gcgtcgtagc cgacggtgta gcggtcgacg taatcgcggt cgacgagcgc
     2521 ttcgtcgatc agcacatgca tgatgccgaa cgcgagcgcg gcatcggtgc ccggcaaaag
     2581 cgcaatgtgc tcgtggcatt tctcggcggt ggcgctgcgg tacgggtcga tcgcgatgag
     2641 ctttgcgccg tgccgtttcg cttcctgcgc gcgcgtccag aaatgcaggt tcgacgcgat
     2701 cggattgctg ccccagatga ggatcaggcg gctccccgcg tattcctcga ggtccatccc
     2761 catcgacgcg ccgatcaccg cggcccagcc ggccttgccc gccgacgcgc agatcgtgcg
     2821 atcgagcagc gatgcgccga gccggtggaa gaagcgccgg tccatcgacg agccctgcag
     2881 cagtcccatc gtcccggcgt agctgtacgg caggatcgcc tgcgggccgt cggccgacgc
     2941 ggcgatcgcc ttgaagcgcg tggtgatttc gtcgagcgct tcgtcccacg aaatgcgcgc
     3001 gaagcggccc tcgcccttcg cgccgacgcg gcgcatcgga taacgcaggc ggtcgggcga
     3061 atacgtgcgc tcgaggtagc gtgcgacctt cgtgcatagc gtgccgccgg tcggcggatg
     3121 gtcggatgcg ccgcggatgt cgacggcgcg gccgtcctcg accgtgacga gcatcgcgca
     3181 ggtgtcggga cagtcgtgcg gacacgctgc gcggacgatt cggcgattcg cgggcccgga
     3241 gctgtcgagc ggcataccac gcattctacg tgagcgatgc acttgccgcg attcggccgc
     3301 aggcacgcgc cgggcaacgg cgccgcgagc ctgcttcgta caccacgaac tgtgcggagc
     3361 acgctgcgcg catgacgcgt cgcgtgttgc gtcaacgact tatcgtcgtc cgatcagcgg
     3421 gaatggtcaa tggcacgctt gctgctcaaa gggtcgcagc cggcgccatc gagacgccga
     3481 acccggacag gagaagatgg tgaattacac gcactacgat tatctggagc tcgcacctgg
     3541 cgcaagcgcg cagcgcgtcg aagccgctta ctgcgcgttg ctcgaacgct ttcaatacgg
     3601 ccacaccgat gcaggccagg acttgtcggg cctcgtgcgc atgatccacg ccgcgtacgc
     3661 cgtgctgtcc gacccggcgc agcgccgcgc ctacgacgag cgtctcacga tcgaagcaca
     3721 ggccgccgac gccgagttgc agtcgcagct cgatgccgca gcgatcaccg gtttcacccg
     3781 cgtgcagcag gtgcccgacg cgctgcttgc gaccattcca gagctcgcgg cctgacgtcg
     3841 gcccgatcgc tttaggtcgc cgggaacatt ttcaatactg caaacagcga tgcggtcggc
     3901 ggcccttctt ccatcgaaac tgcgcgccat cgcgaagcgt ccagggggtc gcaccgcgaa
     3961 gctgcgccgg cccgatgcag cgcgcagacg tccggcgcgc acgccgacac gccgcgcgag
     4021 cgtcgccgcc ggcaatcgac gccggcatcg atcggtgtcc ccgcgccaac ggccgatcgc
     4081 acctgcatcg cgggtgggct ttgcaggcgt cgcgctgcac tgaggcacga gccatcacga
     4141 acaacaaagg gcgagggaga atatcttgga aaagtgtgtc ggctgccagt tctacgatcg
     4201 caacgacgac cacgtcgaag acaagggcgt gcgctggggt aaatgccggc gtggcgtgcc
     4261 gatcgtgcac ccggtttccg ccaaggcgta catggtcgag ggcatctggc cgcacgtgcg
     4321 cgacgacgac tggtgcggcg aatggtcggc gatcaagcgc aagcccgaca cgtcgatgcc
     4381 cgatgcgcga aacctcatgg cgcaaccgtc gacaccgtcg ccgatccgca ccgcgggcct
     4441 gcccggcgga tcgctgatga cacccgtcgc gccggtcggc gaaggcgccg ctgcattgaa
     4501 cggacgcttc ggctccgact gaccctgtca taccgaagtc gccgcggcgg cgaggggccg
     4561 ccgctgcggc aatccgcgtc gcacggcgtt ttctgatccg cgcatcgcgg cctccgcgcc
     4621 acaccgccgc ggtctgctaa agtctcggcc tcgccgcgcc cgtcatgcgt cgcgtcaatt
     4681 cccgatgtcg aatctcctcg aggcgctcgc cgccggcgcg acggtcgtca cgccgaacaa
     4741 tcgtcttgcg cgcgacgtcg cggcccggtt cgacaccgcg cagcgtgcca gcggcaggca
     4801 cgcgtggagc gcggcaaatg cgctgccgtt cacgctgtgg ctcgatcgcc tgtggcgttc
     4861 cgcgctcgcg gcgggggcgc tgcgcgcgcc gcccgtgctg ctcgatgcaa gcgctacgcg
     4921 ggcactgtgg catgcgatcg tcgcgcaaca ccggcacgac tggctgaacg cacacggcgc
     4981 ggcacgtcac gcggccgatg cgtggacgac gtttcatcgt tatcgcgcgc ccggcgagag
     5041 catcgccgac atcgcgtcca atgcgttgca tgacgacccg atcgtgttcg gccggtgggc
     5101 gcagcgctac caggcgcagc ttcgtgcgct caatgcaatc gacgacgcgc aactgcccga
     5161 tgtgctgacg gcgcacgaaa cggcgtcgtc gcttgccgga tgcccaccgg tcaacctgca
     5221 tggattcatc gcgctcacgc cgcagcagcg acggctcgtt gcggcgctgc gcgctgcggg
     5281 cgcgacgatc gacgagtcgc ctgcaaccgg gagcgcggct acgcgatgtc agcgggtggg
     5341 gctggcaacg ccgcgcgacg agctcgtgca cgccttcggc catgcacgcg agcgcctgct
     5401 gcgcgatccg gacgcgcgcg ttgcgattgt cgtcgccgat ctcgacgagc gccgcagcga
     5461 ggtgctggca ctcgccgagg aaacgctttg cgccgagcac ctgctggcaa tcacgccgga
     5521 cgcggtgcgt ccttacggcg tctcgctcgg ggaaccactc gccggggtgc cgctcgtggt
     5581 cgcggctctc gacctgatcg cgctcgcggt gggacgcctg gacgcaacga ccgcgacgag
     5641 cctcgtgcgg acgccctttc ttcccgacgc cgcgacgcag tggacgcggc gcgcgcaagc
     5701 cgagcagcat tggctcgagc tcggcctgcg cgaggtggcg tggccggacg tccttgcggc
     5761 gttgcgcacg gtcgatccga tcctgcatcg ccgcttcgca gtgattccgc cgccaaccca
     5821 tgcgctgcgt gcgccgcgcg actgggcacg cgcatggtcc gactggctcg cagcactcgg
     5881 ctggccgggg agcgcaacgc tgacgagcgg ggagtggcag gcgcgcgaag cgtggtcggc
     5941 ggcactcgca cggttcgcgt cgatcggcag cgtcaccggc acgttgtcgc cggtcgcggc
     6001 cctcgatgcg ttgcgcgcgc ttctcgccga gacgctcttt cagcccgaga tcgcccccgc
     6061 ccctgtccag atcctcggcg tcctcgaggc cgccgggctc acgttcgatt gcgcatggct
     6121 cgcaggattc gatgcgcagc gctggcccgg tacggccgtg tccaatccgc tgctgccgct
     6181 gcgctggcaa caggcgcgcc gcgtgccgcg cgcgcatccc gaagcggtga tcgagcaggc
     6241 ctgcgcaatc acgtcggcgc tgcagaggat cgccccggaa atcgtcgtca gccatgcgat
     6301 gacgatcgac gatgcaccgg cggcgctgtc cccgctcttt gcccattggc cgccgatggc
     6361 cgacgcgctg ctgcctgcgg cgtcgcgcat caccggtctc gtgcctgccg caccgatgga
     6421 aagcgtggcg gagccttcgg cgccaccgct ggtcgacggc gaaacgatcc gcggcggtgc
     6481 gggcctgttc gacagccaaa gcgcgtgtgc cttccaggct ttcgcacgtt accggcttga
     6541 cgcgaggtca tggccgtcct gtcccgacgg gctgtcgccg gccgagcgcg gcgagatgct
     6601 gcatgcgacg ctcaaggcgt tctgggacgc gaccggtgac caatcggcgc tcgcagcact
     6661 cgacgcgcat gcgcttgccc tgcgcatcgc agatgcggtg aaagcgggga aggcgcgcat
     6721 cggttcgccg cgctggcgca cgcttgcgcc tgccgtcgca atcgcggagg cgagccgcct
     6781 cgcggccacg ctgcacgcat ggatcgacga aggcgagcgc acgcgtccgc cgtttcgcgt
     6841 tcgcggccac gagcagcgcg tccgttgcag catcgaaggc atcgcgctgt cgatacgcgt
     6901 cgaccgcatc gacgaattgc ctggcggcgg gctcgcggtc atcgactaca agtcgggcaa
     6961 cgtcgtgtcg ccggcgcgct ggttcatgga acgcccggag ggcatccagc tcgcgatcta
     7021 cgcggcggcc gtcgaggacg caacgcgcga gccggtccgc gcgctcgcgt atgcgcaggt
     7081 gaaggcgggc aacatcgagg ttgccggcct cgccgaggac aacgcgcaat ggcccgcgct
     7141 tgcgtccgtc gattcgactc gtctcgcgct cgccgattgg gccgacgcac gtgcggagct
     7201 cgcggcgcgg gtgcatgcgc ttgcaagcga catccgcaac ggcatcgccg acgtggcacc
     7261 ccgcacgctg gcgatctgcg gttactgcgg actgcagccg ctgtgccgcg tgcagcggct
     7321 cgacgacggc gcgagtgccg ccgacacgcg cgatgaatga cgtcgccgga ctcgcggtgc
     7381 gcgacgacgc ggcgcgcgcc caggcactcg acatcacgcg ctcgtggctc gttcaggcgc
     7441 ccgcgggctc ggggaaaaca tcgctgctga tccagcgctt tctcgcgctg cttgcaaggg
     7501 tcgagcggcc cgagcgcatc gtcgcgatga ccttcacgcg caaggcggcg gccgagatgc
     7561 gcgcgcgcat cgtcgacgca ttgcgcaacg ccgcactggc tccggatgag gcgcggatcg
     7621 gcgcccacga gacgctgacg cgcgcgcttg cgcgccgtgc actcgaccag gaccgggtga
     7681 tgggttggcg cctgctcgag caaccggccc ggctgcgcat cgtgacgatc gacgcgctcg
     7741 ccgcggcact tgcacgccag gtgccgctcg cggcgggcct cggcgcgctg ccgggcttcg
     7801 tcgacgacgc gcgtgcgctg cacgtcgatg ccgcgcgcgc ggcacttgcc gaggccgcac
     7861 cgctcgatcg ccactggcag gccttcctgc agtggcagga caacgacgcg ggtgctgcaa
     7921 cgcgactcat cgcacagatg ctcgctgcgc gcgagcgctg gccgctgccg atgctcgacg
     7981 gcgatcccgc ggtgcttcgt gccgaggtcg aagccgttct cgagctcgag gcgcgggcgg
     8041 cggtggccat cgtgcatgag cgcctgcccg cgtcgctcgc cgcggcgctt ccgcggctcg
     8101 cgcagatggc gctcgacggc ttacacgaac tcggtatcga ttgcgcgcat gccggcgcgc
     8161 tcgccgcgct ttgtcgcgat ggccggttgc ccgacccgca aacgcgcgaa acgtggtgcg
     8221 gacttgcgca gtggctgctg acgcaggaag gaacgttttt ccgcgtggtg accaagcggc
     8281 acggattccc gcgtggcgcc ccgcagacgc aggagcgtgc gcgccgcaag gccgaattcg
     8341 tgcgctggct ggagaaggcg gcgcaggtgc cgggacttgc cgacgcatgg catcgcctgc
     8401 gtgcgctgcc gcccgcgcgg ttcagcgatg acgcctgggc tttcgtcgcg gcggcgatgc
     8461 gggtgctgcc gcgtgccgtc gcggcgctcg acggcgtgtt ccgggcgcgg ggccgggccg
     8521 acttcgccga ggcgacactg cgcgcgctca cggcactcgg cacgccggac gatccgagcg
     8581 atctgctgct cgcgatcgac taccggctgt cgcatcttct gatcgacgaa ttccaggaca
     8641 cgtccagtgc acagctcgca ctgatcggca agctgacctc gggctgggat gccggtgacg
     8701 gacgcacgct tttcgccgtc ggcgacccga tgcagtcgat ctatcgtttt cggcaggccg
     8761 acgtcgcgct gttcctcgac gcgcaggcgc aggcccggat cggcgacgtt ccggtcggcg
     8821 tcgtcgagct cagccgcaat tttcgctcgc agcccgcgat cgtcgacttc gtgaacgacg
     8881 tgttccgggc ggtgctgccg gcgctgtccg atccagcaca ggccgaggcg gcgtatcgcc
     8941 cggcgtatgc cgacccggaa cgcggcgtcg acatcgcccc gacgctcgat ctcgtcggct
     9001 cgcgcgacgc cgaggccggc gtcgtcgtgc agcgcatccg cgatgcgcaa gccgcgggtg
     9061 tcgcagacat cgcgatcctc gtgcgtgcgc gctcgcatgc gcaggcgctg ctgccggcgt
     9121 tgcgccgcgc cggcatcgac tattcggcga tcgacctcga aggattgcac gatcggcttg
     9181 caacacgcga tctcctttcg ctcgcacgcg cgctcgcaca accggccgat cgcctcgcct
     9241 ggctgtcggt gctgcgtgca ccatggtgcg gtctgacgct tgccgacctc catgtcgtcg
     9301 cgaacgccgg cgcatcgacg atcgccgctg cgctcgaggc gcccgacgcg atcgatcggc
     9361 tgtcatccga cggccgcgca cgcgttgcgc ggttccggca atgcgtagca cctgcacttg
     9421 cctcgcgcgg ccaggcgagc ttcaccgcgc gcgtgaaatc ggcgtgggtg gcactcggcg
     9481 ggccggcatg cgcggcatcg ccgctcgatc gcgacggtgc cgatcgcgtg ttcgcgctgc
     9541 ttgccgagca cgagcgcggc gctgacctgc ccgacttcga aacactgcgc gacaccgcgc
     9601 agcggctgtt cgccgacgcc ggcaaggccg cgtctgcggc cgtgcaggtc atgaccttgc
     9661 acaaggcaaa gggattgcag ttcggcgcgg tgatcctgcc cgggctcgat cttcgcgcgg
     9721 gccgcgccga ttcgccgctg ctgcgctgga aggtgcgccg ccaccacgga cagcgcacgc
     9781 tgctgatcgc gccgatgcgg gcgcgcatgg gcgcccgcag cgagccggat cccgtctatg
     9841 cgtggctcgg ggagatcgat gcggccgagg aggccgcgga gctcggccgc ctgctgtacg
     9901 tcggcgcgac gcgcgcgaag cggcgcctgc accttgtcgc cgtcgcttcg gtcgactcga
     9961 agctcgaggc ttgcgaggaa acccgttcgt tcaagcgtcc ggcgcggggc agcgccctcg
    10021 agcgcctctg ggatgcgctc gcggcgtacg tcccgcgcgt tgcaccatcg cccgcgccgg
    10081 ttcgcggcga gcaatcgccg cccgaccatg gcgtgtcgtt gatccgtctg ccgctcggct
    10141 ggcaactgcc cgcgctaccc ccaccgctgc cggtcgcgca tcagcgcgca acgatgcccg
    10201 atgcgccgac gttcgactgg gccgatgcca cggcggcggc aatcggaacg gtggcgcatc
    10261 gcctgctcgc gcaagtcgcc gcggagggcg tcgctgcgtg gggcgcgcgg cggctcgtgc
    10321 tcgagcagcc gcgcattctc gccgagctcg gcagtgaagg ggtcgagccg gatctcgccg
    10381 tggatgcggc aacacgggtc acgacgatcg ttgcgcgcac gctccgcgat ccgcgtggcc
    10441 gctggctctt cgatcccgcg catgtcgacg cgcacagcga atgggcgctt gccggcgacg
    10501 acgacggacg catcgtgcac gtcgtgctcg atcgcagctt cgtcgccgcc ggccggcgct
    10561 acgtcatcga cttcaagacc ggtgcgcatc tcggcggcga tccggcgaca ttcctcgcgc
    10621 gcgaattcga gcgctaccgg ccccagttgc aacgctacgc gcgcatcgtt cgcgcgatcg
    10681 acgcgcgtcc ggtgcgcgtc gcgctctacc atccgctcgt cgaaggcgga tggcaggaac
    10741 acgagttgga ttaaaatagc gggttttcca atcgaggatg ccgcagcggg aaacggctgt
    10801 ttcgccgctg cgggcgaagc gttgcgcgta aagcgggacc tcacgagcgc gccggacggt
    10861 ccggtcgcgc tgacgatcgg caacttcgat ggcgtgcatc gcggccacca ggcgatgctc
    10921 gcccgcctga tcgaagcggc cgaggacctg cggctgccgc ccgccgtgct gaccttcgat
    10981 ccgcatccgc gcgagttctt ctcgccgtcg aacgcaccgc cgcggctgtc gacgctgcgc
    11041 tcgaagctcg cgctctttcg cgcgtacggc gttgcaacga cgttcgtcgc ccgcttcgat
    11101 gcgcggctcg cgtcgcgcac cgccgatgag ttcgtgcgcg gcatcctcga gagccagctc
    11161 cacacgcgct ggctgctggt cggcgaggat tttcgcttcg gacgcggacg cacgggcgat
    11221 ctcgcgtttc tgcgcagcgc ggcgcgcgag ttcagcgtcg aggcgatgga cacgatcggc
    11281 gtcgatgggc agcgcgtgtc gagcaccgcg gtgcgaaacg cgctcgccgc cggcgatctc
    11341 gtgcttgcgg cggcgctcct cggccgcaac tacacgctta cggggcacgt cgcgcacggc
    11401 gacaagatcg gccgctcgct cggctttccg acgatgaacc tgccgctgcg ccatccgccc
    11461 gcgatgacgg gcattttcgc cgtgcgcgtg cacggcatcg gcaacatgcc gcgcacgggt
    11521 gtcgcgagcg tgggcgtgcg tccgaccctc cgcgccggcg gcaggccgat cgcggaggtc
    11581 ttcctgttcg acttcgacca ggcaacctac ggccggcgga tcaccgtgga attcctgcac
    11641 aagctccgcg acgaggcgcg cttccccgac ctcgacgcgc tcaggcagca gatgatgcgc
    11701 gacgtggcgg acgcgcgtga ccatttcgcc gcgaagcgat gatgcccgcc cttcgcgcca
    11761 ttcacgaacc tcacgaagcc tcgacatgcc cgacgacgcc cgcaccgatt acaagccgac
    11821 gctgaacctc cccgacacgc cgtttccgat gcgcggggac ttggctcgcc gcgagccggc
    11881 gtgggtggcc gcgtggcggc gcaacgacgt ctatgcggcg atccgcgcgg cgtcgcgcgg
    11941 gcggccgcgc ttcatcctcc acgacggacc gccgtacgcg aacggcgaca tccacatcgg
    12001 ccacgccgtc aacaagatcc tgaaggacgt gatcgtcaag agccgcacgc ttgcaggctt
    12061 cgatgcgccg tacgttcccg gctgggactg tcacggcatg ccgatcgagg tgcagatcga
    12121 gaagacatac ggcaaggaac tgtccaccgc cgaaacgcag cggctgtgcc gcgcgtacgc
    12181 ggccgagcag atcgagcggc agaaaaagga tttcgagcgg ctcggcgtgc tcgccgactg
    12241 ggaccacccc tacacgacga tgagctacgc caacgaggcc gacgaaatcc gcgcgctcgg
    12301 caaggtgctg gaaaagggct atctgtaccg cgggctgaag cccgtcaact ggtgcttcga
    12361 ttgtcacagc gcgctcgccg aggcggaagt ggagtacgag gaccgcgtcg acaccgcgat
    12421 cgacgtcggt ttcgcactcg acgatccagc cgagcgggcg aagctcgcgc aggcgtttgg
    12481 cctttcccac gcgcccgacg gaccgatcta cgcgatgatc tggacgacga cgccgtggac
    12541 aattccggcg aaccaggcgc tcagtgtgca tcccgacctc gagtacgcgc tggtcgccac
    12601 accgcgcggt cacctggtgc tcgcgaagga tctcgccggc gcctgtcttg cgcgctacgg
    12661 gctcgacggt tcg
//
LOCUS       DASSVX010000125        13096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1221296, whole genome shotgun sequence.
ACCESSION   DASSVX010000125 DASSVX010000000
VERSION     DASSVX010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 13096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13096
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221296"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1226)
                     /locus_tag="VFO53_05445"
     CDS             complement(12..1226)
                     /locus_tag="VFO53_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET8875755.1"
                     /translation="MATAPDLTKLRIDRELAPIRRRRRRRWIWLALAVVLVAAAAAWR
                     ILRPHPQTVQTTPIVTAYPSQQYVVLNATGYVVAQRKAAISSKATGRLEWLGVAEGSR
                     VKTGDLIARIDNRDVVAQAQSAAAAVRAAQANVRQSEVERENAQVEYGRNEELVGKGF
                     ISRSALDTAKARLDRAQAGVASAQANLNVALANARNAQVAVDYTEIRAPFDGVILSKS
                     ANVGDLVTPFSSATDSKGAVVSMADMGTLEVEADVSESSLAKVHVGQPAEIVLDALPD
                     TRFRGHISRMVPTVDRAKATVMTKVKFDAIDPRVLPEMSAKVSFLSQEVTPEQQKPLV
                     SVSKDAVVERDGHAVVFIVRDGHAVAVPVKRGAAIGDTTAIEGNVKTGEKAVEKPAPS
                     IANGALVTTAAK"
     gene            complement(1325..2200)
                     /locus_tag="VFO53_05450"
     CDS             complement(1325..2200)
                     /locus_tag="VFO53_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HET8875756.1"
                     /translation="MGNALFIVWRESAEAMLVVGILYAWLRQRPDAAIGMRYLWGGVA
                     AGAALALILALIMLGIASSLSGNALDYFQLAIMLVASGLIVQMVFWMRKHGRTFKKDL
                     ESSMARNASAANWWGLLIVVALAVGRETAETVVFLYGLGAEKGGIANLPIVLVLGLAA
                     AFATFWLLQQGSRVLSWRMFFRVSEILLLLLAGALLVSGVEKLIALDVLPALVDPVWD
                     ASRLLDDSGRIGGLIASFTGYRSRPALLPLLVLVVYWVVVWWSLGHAGRSAATRAARA
                     PVAAPRDPMPRAERR"
     gene            complement(2222..2566)
                     /locus_tag="VFO53_05455"
     CDS             complement(2222..2566)
                     /locus_tag="VFO53_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET8875757.1"
                     /translation="MNRLLAMGCGSLIALAVFAAAPALAADEAPIKLVARDGVFTPAL
                     IEVPAGKRVRLEVSNEGKTAMEFESRDLKQEKVIPPGGKATLTINAMKPGEYRFFDEF
                     HEKTGQGRFVVK"
     gene            complement(2769..3701)
                     /locus_tag="VFO53_05460"
     CDS             complement(2769..3701)
                     /locus_tag="VFO53_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8875758.1"
                     /translation="MRVSVHSRRAAYAALGVLTLMWGLNWIAMKGALLHADPLTFNVH
                     RTWLAMVVLLAVLIGRRRHFWPHSWRAVVITAFFQTTINFGATTMAVAGGGAGRTSVL
                     VFTMPFWTLLIAWPVLHERVRGMQLAAIAFAFAGLLLVVEPWAWQGHLAPKLWAVLSG
                     FGWAAGTVAMKHFQRERHFDMLSFITWQMVLGVIPLSFLPWMFDAPPTQWSVTQVALL
                     FYVGVIATAGGFLIWMAVLRWLPAGTASLNMFAVPVIALLSSMAVFGERLAANEWAGI
                     ALIGVGLGLITAHALAGGRPRDPLPATPTPVDGG"
     gene            complement(3704..4618)
                     /locus_tag="VFO53_05465"
     CDS             complement(3704..4618)
                     /locus_tag="VFO53_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8875759.1"
                     /translation="MPTQVAIKPATSHFLDVRGERHHVQTWGEPGWPRLVLLHGWMDV
                     GASFQFLVDALARDWYVIAPDLRGFGRSAWQPQGYWFYDYFADLEALLAHYVADAPAR
                     LVGHSLGGNIAMGYAGVRPDRVQRVVSLEGFGIPAEAPATASRKLAKWLDAIADPPSF
                     RTYASIDAVADRLQKKNRRLPRDKADFLAQRWAASFPDGRAELVSDPRHKLPFPHVYR
                     MDEIVAMWRDISAAVLWVAAEDSEIPRWLASHPEGEAGADGLDEVRRRIAHVPGAALA
                     TVADAGHMLHHDQPARVAAVIEPFLAGD"
     gene            complement(4603..4986)
                     /locus_tag="VFO53_05470"
     CDS             complement(4603..4986)
                     /locus_tag="VFO53_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875760.1"
                     /translation="MLEFYSANIADPVLTFVSAAHLPQPIARRLRVRGMLCAIGVALV
                     AAGCATAPFGPSEPPAAAPPLPRNAEPPPPSVNLSGFPLPYRQGYADGCASVSGAERK
                     DAARFGGDPNYRMGWQDGLALCRRK"
     gene            5005..8469
                     /gene="dnaE"
                     /locus_tag="VFO53_05475"
     CDS             5005..8469
                     /gene="dnaE"
                     /locus_tag="VFO53_05475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET8875761.1"
                     /translation="MSSPGFVHLRLHSEYSVADGAVRIDDAIDAALADGMPALALTDL
                     ANQFGLVKFYRAARARGVKPIAGCDAWISNAREPEQPSRVLLLAASRDGYLKLCTWLS
                     RAYLTHQHRGRAELDRAWFDEGTGGLIALSGARDGDVGQALLQGDLDAAKRLAREWAG
                     WFPQRYALEVQRAGHADCEALVQATVALGDSVGIPIVATHPIQFLTADDFRAHEARVC
                     IAEGHTLSDSRRPRRFTAEQYFLTQSQMAEKFADLPEALANSVAIATRCNLTIPLGKN
                     HLPQFPTPAGMSLDDHLRAEAAGGLERRLEQLYPDAAERERRRPEYVARLGFEAKTIV
                     QMGFSGYFLIVADFINWAKSNGVPVGPGRGSGAGSLVAYSLGITDLDPLRYALLFERF
                     LNPDRISMPDFDIDFCQDGRDRVIDYVKHKYGAESVSQIATFGTLAAKAAVRDVGRVL
                     DLPYSFVDGIAKLIPFQPGKTVTLRRRSGESESNVIYAREVEPLLDQREADEEEVREL
                     LSLACELEGLPRNVGMHAGGVLIAPGKLTDFCPLYVQPGAQAPVSQFDKDDVEAIGLV
                     KFDFLGLTTLTILDWTLRYIRKLDPGATIALDTLPLDDASTFDVFRKANTAAVFQFES
                     RGMRDLVKQAQPTRFEDIIALVALYRPGPMELIPDYNARKSGRERVEYLDPRLAPILE
                     PTYGVMVYQEQVMQIAQVIGGYTLGNADLLRRAMGKKKPEEMAQHRDIFVAGAEKNGL
                     ARAKAAQLFDLMEKFAGYGFNKSHSAAYALVAYQTAYFKAHHPAAFMAANLSLVMDDT
                     DKLKTLYDDAIAQGLEVLPPDVNASGYRFEPVDARRIRYGLGGIKGTGSAAIEAIVAA
                     RATDGAFTDLFEFCRRVDKRLVNRRVVEALVRAGAFDAIDTHRAALFASVGLALDAGE
                     RAAATASQVSLFGEDVAPPLQAGPPMRDWTETERLVHEKASLGFYLSGHPFNAHAAEL
                     ATIVRTALSSLQPKNERVLVAGIVTALRVQTSRRGKMAFVTLDDGKGRAEVMVYNETF
                     DTARALLREDQLVVMEIRVMQRMTDDGEAQGLRIVAENIYDLGGVRKRWAKGLRIACN
                     GNASADRLEEILRPFRSGTLPVTVRYANARVGGEVDLPESWRVTPDDALIERLREWLA
                     PENVQVVY"
     gene            8598..9755
                     /locus_tag="VFO53_05480"
     CDS             8598..9755
                     /locus_tag="VFO53_05480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8875762.1"
                     /translation="MSRLLAMAVLARMPLGTVTLAMLLHVRALTGSFAAAGAAVGAYL
                     GASAITAPLIGRFIDRHGPRVALLVTGIVCPAALLVLWLAKPLALPTPTLWIVAGVAG
                     AFSPPISVLTRTMWRHRFDDESARRVAFALDGVLIEFSFTAGPALIALLLAIGSPAVA
                     FGAAFVFMSIAVPTFVASPALRYWHHDPHAQRRLLGPLTEPRLIAVYATTFLLTLGLG
                     LLEVAYPGFAVKVHRPPLAGLLIAANSIGSAAGGLVYGGLHIALARERQLRLAVALLV
                     LPLAVQGLIASAGLLAVVAFIAGLCIAPTLTIVTLLISSYAPARYATEAFTWSATCIV
                     SGIGAGNALGGLLLERYDPGIVFGASGAAALLAATCAWTLRPARAQRVHAQ"
     gene            9760..11298
                     /locus_tag="VFO53_05485"
     CDS             9760..11298
                     /locus_tag="VFO53_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018857657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET8875763.1"
                     /translation="MSPRSVRAPTGVSPRRRVVLACVMLALFMAAVEVTIVATALPQI
                     VGKLGGFSLYTWVFSGFLLTQASTILVFGKLADLYGRRPVLIGGIVVFLIGSTLCGLA
                     WSMPALIAFRLLQGLGAGSIQPVAATIVGDLYTPQERPRVQGWLSSVWGFAAIVGPLA
                     GGLIVEVLSWHWIFWINVPVGLLTIVGLSLFLREDVGHRPHAIDYVGVALFCVAICAL
                     LVAVMEGGVDWPWRSLPTLLLVALFAVALGLYVARERRAPEPMIDFALWRARLTASVN
                     VSSLFAGAAIIGVTSFVPIYVQGVLGRSAIVAGFALTAMSVGWPLASTLSGRFLRALE
                     ARGTARLGGGVLFAGALMLATMPSGVGPIRVGAGAFIVGFGMGLLNTTFIILIQASVP
                     WAQRGSATASNIFSRMLGSTFGAAALGAVLNASLSSHFNGPGAGLASLDTVRRMFDSA
                     DAVALPPAQRAALQAALGAGLTHVFAALAVFGTLTMLATWFVPRRTVFAPADAQAAPR
                     AARR"
     gene            complement(11355..12863)
                     /locus_tag="VFO53_05490"
     CDS             complement(11355..12863)
                     /locus_tag="VFO53_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018494354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8875764.1"
                     /translation="MTMDRARRFFGMYVDGGYRPAQSGNVLAVENPKDRSVVAEVAEG
                     AAVDIDAAVAAARNALPGWSSLSGAQRGDLMHRAADALARRLAELVDVEIDQTGRPRC
                     EMSAQLARLPEWFRYFGAVARTHEDTVPPFGGDFLNYTKRVPLGVVGHITPWNHPLLI
                     LTKKVAPSMAAGNTMVVKPSELAPITALLLGEVLKEAGIPDGVYNVVPGFGPSAGLAL
                     AAHPHIRKIDLTGGTPTGKALAAIAGANLTRISAELGGKAAVIVFPDIAIDRAVAAAL
                     FAAFIASGQTCVQGARLLVHRSIHDAVVRELVQRAQAIRLGDPRDARTQMGPLISARQ
                     RDVVEQYVRIGVDEGATLAAGGRRPRGLPFEAGYYYLPTVFTNVTGAMRIAQEEIFGP
                     LVCVIAFDTEEEAVALANGTAYGLAASVWTRDLARAHRVAGALECGVTWINDHHRIDP
                     ASPWGGFRMSGLGRENGLVAYEDYTQIKNVIVNLCDAPFDWYAGDGAPARYS"
BASE COUNT         2039 a         4530 c         4377 g         2150 t
ORIGIN      
        1 cgtttccggc gtcacttcgc cgccgtcgtg acgagtgcgc cgttcgcaat cgatggcgcg
       61 ggcttttcca cggccttttc accggtcttg acgttgcctt cgatcgcggt cgtgtcgcca
      121 atcgccgctc cccgcttcac ggggacggcc accgcatggc cgtcgcgaac gatgaacacg
      181 accgcgtgcc cgtcgcgctc gaccacggcg tccttgctta ccgagacgag cggcttctgc
      241 tgctcgggcg tcacttcctg cgacaggaag ctcaccttcg cgctcatttc cggaagaacg
      301 cgtggatcga tcgcgtcgaa cttcaccttc gtcatcaccg tcgccttggc gcgatcgacg
      361 gtcggcacca tccggctgat gtggccgcga aagcgcgtat cgggcagcgc gtcgagcacg
      421 atctcggcag gctgtccgac atgcaccttg gcgaggctcg attcggacac gtcggcctcg
      481 acttcgagcg tgcccatgtc ggccatcgaa accaccgcgc ccttcgaatc ggtcgcgctg
      541 gagaacggcg tcaccaggtc gccgacattc gcgctcttcg acaggatgac gccgtcgaac
      601 ggtgcgcgta tctcggtgta gtcgaccgcc acttgcgcgt tgcgcgcatt ggcgagcgcg
      661 acgttgagat tcgcctgcgc gcttgcgaca cccgcctgcg cgcgatcgag ccgggccttc
      721 gcggtatcga gcgccgagcg cgaaatgaag cccttgccga cgagctcctc gttgcgcccg
      781 tactcgacct gcgcgttctc gcgctcgact tcgctctgcc gcacgttggc ctgcgcggcg
      841 cgcaccgcag ccgccgcgct ttgcgcctgc gcgacgacgt cgcggttgtc gatgcgcgcg
      901 atgagatcgc cggtcttcac gcgcgagccc tcggcgacgc cgagccactc gagccggccg
      961 gtggccttcg acgagatggc cgccttgcgc tgcgccacga cgtaccccgt cgcgttcagc
     1021 acgacgtatt gctgcgacgg atacgccgtg acgatcggcg tcgtctgcac cgtttgtgga
     1081 tgcggccgca ggatgcgcca ggcggcagcg gccgcgacga gcaccacggc gagtgcgagc
     1141 catatccatc tgcggcggcg gcgccggcgg atcggcgcga gctcgcgatc gatgcgcagc
     1201 ttggttagat cgggtgcggt ggccattaaa cgaagtgccg gccatgacgg ccggcacgtt
     1261 tcattttacc gcgatacggg cgatgcccgc gccaggcacc ggccgtgcag agcggcagcc
     1321 gaggtcagcg gcgctcggcg cgcggcatcg gatcgcgcgg cgccgcaacg ggcgcgcgcg
     1381 ctgcgcgtgt tgctgccgat ctccccgcat gccccagcga ccaccagacg acaacccagt
     1441 agacgacgag cacgagcagc ggcaacagcg ccggacgcga gcggtaaccg gtgaacgacg
     1501 cgatcaagcc gccgatgcgc cccgagtcgt cgagcagccg cgacgcatcc cataccggat
     1561 cgacgagcgc gggcaacacg tcgagcgcga tcagcttctc gacaccggag acgagcagcg
     1621 caccggccag aagcagcagc aggatttcgc tgacgcggaa gaacatccgc cacgacagca
     1681 cgcgcgagcc ctgctgcagc agccagaacg tcgcgaatgc cgcggcaagc ccgagcacca
     1741 gcacgatcgg cagattggcg atgccgccct tttcggcgcc gagaccatag aggaagacca
     1801 cggtttccgc ggtctcgcgg ccgacggcaa gcgcaacgac gatcagcagc ccccaccagt
     1861 tcgcggcact ggcgttgcgc gccatgctcg actcgagatc cttcttgaac gtgcggccgt
     1921 gcttgcgcat ccagaacacc atctgcacga tcaggcccga cgcgaccagc atgatcgcga
     1981 gctggaagta atcgagtgca ttgcccgaca gcgacgacgc gatgccgagc atgatcagcg
     2041 caaggatgag cgccagcgcc gcacctgcgg caacgccgcc ccacaggtag cgcatgccga
     2101 tggccgcgtc gggccgctgc cgcagccacg cgtacaggat gccgacgacc agcatggctt
     2161 cggccgattc gcgccagacg atgaaaagtg cgttgcccat ggccgtcgat gcttgctcga
     2221 gctatttcac gacgaaccgg ccctgcccgg tcttttcgtg gaattcatcg aagaaccggt
     2281 attcgccggg cttcatcgcg ttgatcgtga gcgtcgcctt gccacccggc ggaatcacct
     2341 tttcctgctt gagatcgcgg ctttcgaatt ccatcgcggt cttgccttcg ttgcttacct
     2401 cgagacgcac gcgcttgccg gcaggcacct cgatcaacgc cggcgtgaac acgccatcgc
     2461 gcgcgacgag cttgatcggc gcctcgtccg cggcgagcgc gggcgcggca gcgaagaccg
     2521 ctagtgcgat cagggacccg caccccatgg cgagcagccg gttcatacga aaaagctcct
     2581 tatcgagtgg accgccggaa gaggcagtcc gtgcggcgag catagcaaag gaatcgttga
     2641 atgtaaataa taaccattat cattggcggc gcactcggag cggcgcactc ggagcggcgc
     2701 actcggagcg gcgcactcgc gctcaatatc gctatcgcgc gtggtccgcg cggacggcgt
     2761 ggcaaccgct acccgccatc gacgggcgtc ggcgtggcgg gcagcgggtc gcggggccgg
     2821 ccgccggcga gcgcgtgcgc ggtgatgagt ccgagcccca cgccgatcag cgcgatgccg
     2881 gcccattcgt tcgcggccag ccgctcgccg aagaccgcca tcgacgacag gagcgcaatg
     2941 accggcaccg cgaacatgtt gagcgaggcc gtgcccgcgg gaagccagcg aagcaccgcc
     3001 atccagatca ggaaaccgcc ggccgttgca atcacgccca cgtagaagag cagcgcgacc
     3061 tgcgtcacgc tccactgcgt cggcggcgcg tcgaacatcc acggcaggaa gctgagcggg
     3121 atcacgccga gcaccatctg ccaggtgatg aagctcagca tgtcgaaatg gcgctcgcgc
     3181 tggaagtgct tcatcgcgac ggtgcccgcg gcccagccga agccggacag caccgcccac
     3241 agcttggggg cgagatggcc ttgccacgcc cacggctcga ccacgagcag cagcccggcg
     3301 aaagcgaacg cgatcgccgc gagctgcatc cctcgcacgc gctcgtgcag caccggccac
     3361 gcgatcagca gcgtccagaa cggcatcgtg aacacgagca ccgacgtgcg tcccgcaccg
     3421 ccgccggcaa ccgccatcgt cgttgcgccg aaattgatcg tcgtctggaa gaacgccgtg
     3481 atgacgacgg cgcgccagct atgcggccag aagtggcgcc tgcgcccgat gagaacggcg
     3541 agcagcacga ccatggcgag ccacgtccga tgcacattga acgtgagcgg gtccgcgtgc
     3601 agaagcgcgc ccttcatcgc gatccagttg aggccccaca tgagcgtgag cacgccgagc
     3661 gccgcatacg ccgcgcggcg cgaatgcacg ctgacgcgca ttttcaatcg ccggcgagaa
     3721 agggctcgat caccgccgcc acgcgggccg gctgatcgtg gtgcagcatg tggcctgcat
     3781 cggcaaccgt cgcaagcgcg gcgcccggca catgcgcaat gcgccggcgc acctcgtcga
     3841 gaccatcggc gcccgcctcg ccctcgggat ggctcgcgag ccagcgcggg atttccgaat
     3901 cctcggcggc gacccacagc accgccgcgc tgatgtcgcg ccacatcgcg acgatctcgt
     3961 ccattcgata gacgtgcggg aacggcagct tgtggcgcgg atcggagacg agctcggcgc
     4021 ggccgtcggg aaacgacgcc gcccaccgtt gcgcaagaaa gtcggccttg tcgcgcggca
     4081 ggcgccgatt cttcttctgc agcctgtcgg caaccgcgtc gatgctggca tacgtgcgaa
     4141 acgacggcgg atcggcgatc gcgtcgagcc acttcgccag cttgcgcgat gccgtggcgg
     4201 gcgcttcggc cggaatgccg aagccttcga gcgaaacaac gcgctgcacg cggtcgggcc
     4261 gcacgcccgc ataacccatc gcgatgttgc cgcccaggct gtggccgacg aggcgcgcgg
     4321 gcgcatcggc gacgtagtgc gcgagcaagg cctcgagatc ggcgaaatag tcgtagaacc
     4381 agtagccctg cggctgccat gccgagcggc cgaatccgcg caggtcgggc gcgatcacat
     4441 accagtcgcg cgcaagcgcg tcgaccagga actggaacga cgcgccgacg tccatccagc
     4501 cgtgcagaag cacgagacgc ggccacccgg gctcgcccca cgtctgcaca tgatggcgct
     4561 cgccgcgaac gtcgaggaaa tgcgacgtcg ccggcttgat cgctacttgc gtcggcaaag
     4621 cgcgagcccg tcctgccagc ccatccggta gttaggatcg ccgccgaagc gcgcggcatc
     4681 cttgcgctcg gcgccgctca cgcttgcgca accgtcggcg tagccctgcc ggtagggcaa
     4741 gggaaatccc gacagattga ccgaaggcgg cggcggctcg gcgttgcgtg gcaacggggg
     4801 cgctgccgcg ggcggctccg atggcccgaa gggtgcggtt gcgcaaccgg cggcaacgag
     4861 cgcgacgccg attgcgcaca gcatgccgcg cacgcgcaat cgtcgtgcaa tcggctgcgg
     4921 caaatgcgct gcgctgacga acgtcaagac cggatcggcg atgttggcag agtagaattc
     4981 tagcatcgct cgctgctgca tctcatgtca tcacccggct tcgtccacct gcgcctgcat
     5041 agcgagtact cggtcgccga cggcgcggtg cgcatcgacg atgcgatcga cgcggcgttg
     5101 gccgacggca tgcccgcgct ggcgctcacc gatcttgcga accagttcgg gctcgtcaag
     5161 ttctatcgcg cggcacgcgc ccgcggcgtc aagccgatcg ccggctgcga tgcgtggatc
     5221 tccaatgcgc gcgagcccga gcagccgtcg cgcgtcctgc tgcttgcggc gagccgcgac
     5281 ggctatctca agctttgcac ctggctttcg cgtgcgtacc tcacgcatca gcatcgcgga
     5341 cgcgccgagc tcgatcgcgc ctggttcgac gagggcaccg gcgggctgat cgcgttgtcg
     5401 ggcgcgcgcg acggcgacgt cgggcaggcg ctgctgcagg gcgacctcga cgcggcgaag
     5461 cgtcttgcac gcgaatgggc cggctggttc ccgcagcgct acgcgctcga ggtgcagcgc
     5521 gcggggcacg ccgactgcga agcgctggtt caggcgacgg tcgcgctcgg cgacagcgtc
     5581 ggcataccga tcgtcgcgac gcatccgatc cagttcctga cggccgacga ttttcgcgcg
     5641 cacgaggcgc gcgtgtgcat cgccgaagga cacacgcttt ccgacagccg ccgcccgcgc
     5701 cgcttcacgg cagagcagta tttcctgacg cagtcgcaga tggccgaaaa attcgccgac
     5761 ctgcccgaag cgctggcgaa cagcgttgcg atcgccaccc gctgcaacct gacgattccg
     5821 ctcggcaaga atcatctgcc gcagtttccg acgcccgccg gcatgtcgct cgacgatcat
     5881 ctgcgcgccg aagcggcggg cggcctcgag cgccggctcg aacagctcta tcccgacgcc
     5941 gccgagcggg agcggcggcg tcccgaatac gtcgcgcgcc tcgggttcga agcgaagacg
     6001 atcgtgcaga tgggcttctc aggctacttc ctgatcgtcg ccgatttcat caactgggcc
     6061 aagtccaacg gcgtgccggt gggtccgggg cgcggatcgg gtgcaggatc gctggtcgcg
     6121 tattcgctcg gcatcaccga tctcgatcca ctgcgctacg cgctgctgtt cgagcggttc
     6181 ctgaatcccg atcgcatctc gatgcccgac ttcgacatcg acttctgcca ggacggccgc
     6241 gatcgcgtca tcgattatgt gaagcacaag tacggcgccg agtcggtgtc gcagatcgcg
     6301 acgttcggca cgctggcggc gaaggcggcc gtgcgcgatg tcgggcgcgt gctcgacctg
     6361 ccgtattcgt tcgtcgacgg catcgccaag ctgatcccgt tccagcccgg caagacggtc
     6421 acgctgcgcc ggcgcagcgg cgaatccgaa tccaacgtga tctatgcgcg cgaggtcgag
     6481 ccgcttctcg accagcgcga ggccgacgag gaagaggtgc gcgagctgct gtcgctcgcc
     6541 tgcgagctcg aaggcctgcc gcgcaacgtc ggcatgcacg cgggcggcgt gctgatcgcg
     6601 cccgggaagc tgaccgattt ctgtccgctg tacgtgcagc cgggcgcgca ggcgccggtg
     6661 tcgcagttcg acaaggacga cgtcgaggcg atcggtctcg tcaagttcga cttcctgggc
     6721 ctgaccacgc tgacgattct cgactggacg ctgcgctaca ttcgcaagct cgatcccggt
     6781 gcgacgatcg cgctcgacac gctgccgctc gacgacgcgt cgaccttcga cgtcttccgc
     6841 aaggcgaaca cggcggcggt gttccagttc gaatcgcgcg gcatgcgcga tctcgtcaag
     6901 caggcgcagc cgacgcgctt cgaggacatc atcgcgctgg tcgcgctgta tcggccggga
     6961 ccgatggagc tcatccccga ctacaacgcg cgcaagagcg gccgcgagcg cgtcgaatat
     7021 ctcgatccgc ggctcgcgcc gatcctcgag ccgacgtacg gcgtgatggt ctaccaggaa
     7081 caggtgatgc agatcgcgca ggtgatcggc ggctacacgc tcggcaacgc cgacctgctt
     7141 cgtcgcgcga tgggcaagaa gaagcccgag gagatggcgc agcaccgcga catcttcgtc
     7201 gccggcgccg agaagaacgg gctcgcgcgc gccaaggccg cgcagctctt cgacctgatg
     7261 gaaaagttcg ccggctacgg cttcaacaag tcgcattcgg ccgcgtacgc gctcgtcgcc
     7321 taccagaccg cgtacttcaa ggcgcatcac ccggcggcgt tcatggcggc gaacctgtcg
     7381 ctcgtgatgg acgacaccga caagctgaag acgctctacg acgacgcgat cgcgcaggga
     7441 ctcgaggtcc tgccgcccga tgtcaacgca tccggttacc gtttcgagcc ggtcgacgcg
     7501 cgccgcatcc gctacggact gggcgggatc aagggcacgg gcagcgcggc catcgaggcg
     7561 atcgtcgccg cacgcgcgac ggacggcgcg ttcaccgatc tgttcgagtt ctgccgccgc
     7621 gtcgacaagc gcctcgtcaa ccggcgcgtc gtcgaggcgc tggtgcgcgc cggcgcattc
     7681 gacgcaatcg acacgcaccg tgcagcgctt ttcgcttcgg tcggtctcgc tctcgatgcc
     7741 ggtgagcgtg cggcggcgac ggcgtcgcag gtgtcgctgt tcggcgaaga cgtcgcgccg
     7801 cctctgcaag ccggaccgcc gatgcgcgac tggaccgaaa ccgagcggct cgtgcatgag
     7861 aaggcgtcgc tcggcttcta tctttccggg catccgttca acgcgcacgc cgccgagctt
     7921 gcaacgatcg tgcgcaccgc gctttcgagc ctgcagccga agaacgagcg cgtgctcgtc
     7981 gccgggatcg tcacggcgct gcgcgtgcag acgagccggc gcggcaagat ggcgttcgtc
     8041 acgctcgacg acggcaaggg ccgcgcggaa gtgatggtct acaacgagac gttcgacacc
     8101 gcccgcgcgc tgctgcgcga ggaccagctc gtggtcatgg aaatacgcgt gatgcagcgc
     8161 atgaccgacg acggcgaggc gcagggcctg cgcatcgttg ccgagaacat ctacgacctc
     8221 ggcggcgtgc gcaagcgctg ggcgaagggt ctgcgcatcg cgtgcaacgg caatgcgtcg
     8281 gcagatcgcc tcgaggagat tctgcgtccg ttccgttcgg gcacgctgcc ggtcaccgtc
     8341 cgctacgcca acgcgcgcgt cggcggcgag gtcgacctgc ccgaatcgtg gcgcgtgacg
     8401 cccgacgacg cgctgatcga gcgactgcgc gaatggctcg cgcccgagaa cgtccaggtc
     8461 gtctactgaa aagtggggtc agagacgact ttttttcgcg atgccggaat ccgggacact
     8521 tgcggcgaaa gtcgtctctg accccgattt tccgcgcggc cgccgcgcaa tacgcgtggt
     8581 tcttccggct gcccgatgtg tcgcgcctcc tcgcgatggc cgtgctcgcg cggatgccgc
     8641 tcggcacggt gacgctcgcg atgctgctgc acgtgcgggc gctgacgggc tcgttcgcag
     8701 cagcaggcgc cgcagtcggc gcctatctcg gcgcatcggc cataacggcc ccgctcatcg
     8761 gccgcttcat cgaccgccac ggaccccgcg tggcgctgct cgtcacgggc atcgtgtgtc
     8821 ccgcggcgct gctcgtgctg tggctcgcga agccgcttgc gcttcctacg ccgacgctgt
     8881 ggatcgtcgc cggcgtcgcg ggcgccttct cgccgccgat cagcgtgctg acgcgcacga
     8941 tgtggcgtca ccgcttcgac gacgagtcgg cgcgccgcgt cgcgtttgcg ctcgacggcg
     9001 tgctcatcga gttttcgttc accgcgggcc cggcgctgat cgcgctgctg ctggcgatcg
     9061 gctcgccggc cgttgccttt ggcgcggcat tcgttttcat gtcgatcgcg gtaccgacgt
     9121 tcgtcgcatc gcccgcgctt cgctactggc atcacgaccc gcacgcgcaa cggcggctgc
     9181 tcgggccgct gaccgagccg cggctcatcg ccgtctatgc gacgacgttc ctgctgacac
     9241 tcgggctcgg cctgctggag gtcgcctacc ccggcttcgc cgtgaaggtg caccggccgc
     9301 cgcttgcggg cctgttgatt gcggcgaact ccatcggcag tgcggcgggc ggactcgtgt
     9361 acggcggcct gcacatcgcc ctcgcacgcg agcgccagct tcggctggcg gttgcgctgc
     9421 tcgtgctgcc gctcgcagtg cagggcctca tcgcgtcggc cgggctgctc gcagtcgtcg
     9481 cgttcatcgc cggactgtgc atcgcgccga cgctgacgat cgtcacgctg ctgatctcgt
     9541 cgtatgcacc ggcgcgctat gcgaccgagg cgttcacctg gtcggcgacg tgcatcgtca
     9601 gcggcatcgg tgccggcaac gcgctcggcg ggctgctgct cgagcgctac gatccgggga
     9661 tcgtgttcgg cgcgtcgggc gcggccgcgc tgctggccgc gacctgtgcg tggacgctgc
     9721 gaccggcacg tgcgcagcgc gttcatgcac aatagatcgg tgtctccccg ttccgtgcgc
     9781 gcgccgaccg gcgtgtcacc ccgccgtcgc gtcgttctcg cgtgcgtcat gctcgcgctt
     9841 ttcatggcgg cggtcgaagt cacgatcgtc gccaccgcac tgccgcagat cgtgggcaag
     9901 ctgggcggct tctcgctcta cacatgggtg ttctcgggct tcctgctgac gcaggcatcg
     9961 acgatcctcg tgttcggcaa gctcgccgac ctgtacggtc gccgtccggt gctgatcggc
    10021 ggcatcgtcg tctttctcat cgggtcgacg ctgtgcggcc tcgcgtggtc gatgccggcg
    10081 ctgatcgcct tccggctgtt gcagggactc ggtgcgggat cgatccagcc ggtggcggcg
    10141 acgatcgtcg gcgatttgta cacaccgcag gagcgtccgc gcgtgcaggg ctggctgtcg
    10201 agcgtctggg gattcgccgc catcgtcggc ccgcttgccg gcggactcat cgtcgaggtg
    10261 ctgtcgtggc actggatctt ctggatcaac gtgcccgtcg gactgctcac gatcgtcggt
    10321 ctgtcgctct tcctgcgcga agacgtcggc caccggccgc atgcgatcga ctacgtcggc
    10381 gtcgcgctct tctgcgtcgc gatctgcgca ctgctcgtgg ccgtcatgga aggcggcgtc
    10441 gactggccgt ggcggtcgct gccgacgctg ctgctcgtcg cgctgttcgc cgttgcgctc
    10501 gggctctacg tcgcgcgcga acggcgcgca cccgagccga tgatcgattt cgcgttgtgg
    10561 cgcgcgcgac tgaccgcgag cgtcaacgtg agctcgcttt tcgccggcgc cgcgatcatc
    10621 ggcgtgacgt cgttcgtgcc gatctacgtg cagggcgtcc tcggccgctc ggccatcgtc
    10681 gcgggattcg cgctgaccgc catgtccgtc ggctggccgc tcgcgtcgac attgtcggga
    10741 cggttcctta gagcgctcga ggcgcgcggc acggcgcgtc ttggcggcgg cgtgcttttc
    10801 gcgggcgcgc tgatgctcgc gaccatgccc tcgggtgtgg ggccgatccg cgtgggtgcc
    10861 ggtgcgttca tcgtcggttt cggcatgggg ctgctcaata cgacgttcat cattctcatc
    10921 caggcgagcg tgccgtgggc gcagcgcggc agcgcaaccg cgtccaacat tttctcgcgc
    10981 atgctgggca gcacattcgg cgcggcagca ctcggcgccg tcctcaatgc gagcctcagc
    11041 agccacttca acggacccgg cgcgggcctc gcctcgctcg acacggtgcg ccgcatgttc
    11101 gatagcgccg acgccgtcgc gctcccgccg gcgcaacgcg cagcgctgca ggccgcgctc
    11161 ggcgcgggcc tcacgcacgt cttcgcagca cttgccgtgt tcggcacgct gacgatgctt
    11221 gcgacatggt tcgtgccgcg gcggaccgtc ttcgcgcctg ccgatgcgca ggccgcgccg
    11281 cgtgccgcac ggcgatagcg gcccggccgc ttgccacgtt cgctacgcgt cgcacgaggc
    11341 cgccgcctcc cctttcagct atagcgcgcg ggcgcgccgt cgccggcgta ccagtcgaag
    11401 ggcgcatcgc acagattcac gatcacgttc ttgatctgcg tgtagtcctc gtacgcgacg
    11461 agcccgtttt cacgaccgag cccgctcatc ctgaacccgc cccatggcga agccggatcg
    11521 atgcggtgat gatcgttgat ccaggtgacg ccgcattcga gggcgccggc gacgcggtgg
    11581 gcacgcgcga gatcgcgcgt ccacacgctg gccgccaatc cgtacgcggt gccattcgcg
    11641 agcgcgacgg cctcctcttc ggtgtcgaac gcaatcacgc agaccagcgg tccgaagatt
    11701 tcctcctggg cgatgcgcat ggcgccggtc acgttcgtga atacggtcgg cagatagtag
    11761 tagcccgcct cgaacggcaa ccctcgaggt cgccggcccc ccgcggccag cgtcgcgcct
    11821 tcgtcgacgc cgatgcggac gtactgctcg acgacgtcgc gctgccgtgc cgatatcagc
    11881 ggtcccatct gcgtgcgggc atcgcgcggg tcgccgaggc gaatcgcctg cgcgcgctgc
    11941 acgagctcgc gcaccacggc gtcgtgtatc gatcggtgca cgagcaggcg cgcgccctgg
    12001 acgcacgtct gtccgctggc gatgaacgcg gcgaagagcg ctgccgccac cgcgcggtcg
    12061 atcgcgatat cgggaaagac gatcaccgca gccttgccgc cgagctccgc cgagatgcgt
    12121 gtgaggttcg ccccggcgat agcggcgagc gccttgcccg tcggtgtccc gcccgtcagg
    12181 tcgatcttgc gaatgtgcgg atgcgcggcg agtgccagac ccgcgctggg accgaagccg
    12241 ggcaccacgt tgtagacacc atcgggaatg ccggcctcct tcagcacctc gccgaggagc
    12301 agcgcggtga tcggtgcgag ctcgctgggc ttcacgacca tcgtgttgcc cgccgccatc
    12361 gacggcgcga ccttcttggt caggatcagc agcggatgat tccacggcgt gatatgaccg
    12421 accacgccga gcggcacgcg cttcgtgtag ttcaggaagt cgccgccgaa cggaggcacc
    12481 gtgtcctcat gcgtgcgtgc cacggcgccg aaataccgga accattccgg caggcgcgcg
    12541 agttgcgccg acatctcgca acgcggtcgg ccggtctggt cgatctcgac gtccacgagt
    12601 tccgccaggc gacgcgcaag cgcatcggct gcccggtgca tcaggtcacc gcgctgcgcg
    12661 cccgatagcg aagaccagcc cggtaatgcg ttgcgtgccg cagcgaccgc cgcatcgatg
    12721 tcgaccgcgg cgccttccgc gacctcggcg acgacgctgc gatccttcgg gttctcgaca
    12781 gcgagcacgt tgcccgactg cgccggccgg tagccgccat cgacgtacat tccaaagaac
    12841 cgccgcgctc gatccatcgt catgggttcc tcaccgggtt cctcaccgaa ttcctcaccg
    12901 ggtgtcaagg caaagtagaa atgtccggtt ccgggcaaag tagaaatgtc cggtacgggt
    12961 tcatgttgca atcaggatcg aaccacgaat tcagggctta atgctccgat cggcgttgtt
    13021 ctccacgggt ggtttgcagc gggtcgcacg gggcgcggct tggccaagac ttccttgcgg
    13081 gtctgcgcgg gcggtg
//
LOCUS       DASSVX010000126         2800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_521339, whole genome shotgun sequence.
ACCESSION   DASSVX010000126 DASSVX010000000
VERSION     DASSVX010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521339"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VFO53_05495"
     CDS             <1..855
                     /locus_tag="VFO53_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875765.1"
                     /translation="RKRGKGPAGDVPTLTDSLDAGESSLAPTVILANTQAPADPIMLE
                     RPGDADRAAEAAETAETAATDPMPLDTLGDLPVLTDAVLDWPPGEPPIEARDIGAELG
                     DAFPASAAEAEVAAAELPPPAVLEREADVDLSAPDTGEAPVQQIGAVPGEPRVPFLDD
                     DFILEIPPPAEPARATAAPSAPIPANFGASTPAAGGHEWDALAEEIRMQVLQRLDLFT
                     DTGLREQLGARLSPIVERASAQLIETINRELGELVRGYVAEAIEREIESWRNQNPSTP
                     RSKSGSEP"
     gene            complement(900..1292)
                     /locus_tag="VFO53_05500"
     CDS             complement(900..1292)
                     /locus_tag="VFO53_05500"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET8875766.1"
                     /translation="MSALVFHMIGGAPDPVAPFSHAVEADGWVFLTGQMPFTTSSNTS
                     AYPDTIEAQTHQVMQNLRAVLQGCALGLEHVVSVRIFLRHFDEHYERMNATYASYFPA
                     DRRPARTCVGVTGLARGALVEIDMIARR"
     gene            1606..2664
                     /gene="aroF"
                     /locus_tag="VFO53_05505"
     CDS             1606..2664
                     /gene="aroF"
                     /locus_tag="VFO53_05505"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET8875767.1"
                     /translation="MIIVMQPDAGDAEIAAVEAKIRAHGLDVHISRGTERTLIGAIGD
                     ERKLDPEMFDPLPGVERSLHILKPYKLVAREWHKASTIVDVCGARIGGNEVQVIAGPC
                     SVETVPQMQAAAAGVASAGARLMRGGAFKPRTSPYTFQGKGVEGLTMLKDAAARHDLP
                     IVTELMDVRMLDTFLEHGVDVIQIGTRNMQNFDLLKEVGKVNVPVILKRGMSATISEW
                     LMAAEYVAAGGNHRIMLCERGIRTFETAYRNVLDVTAVAVLKKETHLPVIIDPSHAGG
                     KAWMVPALSCAAIAAGADGLLIEVHPCPAEAWCDADQALSIDEFATLMKRLGAVAEAM
                     GRSLAPAREVTRTELATA"
BASE COUNT          501 a          905 c          943 g          451 t
ORIGIN      
        1 cgaaaacgtg gcaagggtcc cgcgggcgac gtccccacat tgaccgattc gctcgacgcg
       61 ggcgaatcgt cgctcgcccc gacggtgatt ctcgcgaaca cacaagcgcc cgccgatccc
      121 atcatgctcg agcggccggg cgacgccgat cgcgccgcgg aagccgccga aaccgccgaa
      181 accgccgcga ccgatccgat gccgctcgat acgctcggcg acctgccggt gctgaccgat
      241 gccgtgctcg attggccccc cggcgagccg cccatcgagg cgcgagacat cggcgccgag
      301 ttgggcgacg cgttcccggc gtcggcggcc gaagccgagg ttgccgcggc cgagttgccg
      361 cccccggccg tgctcgagcg cgaggccgat gtcgatttga gcgcacccga caccggcgaa
      421 gcgccggtgc agcaaatcgg tgccgtgccc ggcgaaccgc gggtaccgtt cctcgacgac
      481 gatttcatcc tcgagattcc acctcccgcc gagcctgcac gagctacggc ggcgccatcg
      541 gcgccgatcc ccgcgaactt cggtgcatcg acgcctgccg ccggcggtca cgagtgggat
      601 gcgcttgccg aggaaatccg gatgcaggtg ctgcagcggc tcgatctgtt caccgacacc
      661 ggactgcgcg agcaactcgg cgcgcggctg tcgccgatcg tcgagcgtgc cagcgcacaa
      721 ctgatcgaga cgatcaaccg tgagctcggc gagctcgtgc gcggctacgt cgccgaggcg
      781 atcgaacgcg agatcgagag ctggcgcaac cagaatccat cgacgccgcg atcgaaatcg
      841 gggtcagagc cgtaatagtt ccggcgcagt agttaattac ggctctgacc ccgatttcgt
      901 cagcgacgcg cgatcatgtc gatctcgacg agtgcgccgc gtgcaagccc cgtcacgccg
      961 acgcaggtcc gcgccggccg cctgtcggct ggaaagtagc tcgcgtacgt cgcgttcatt
     1021 cgctcgtagt gctcgtcgaa atgacgcagg aagatacgca ccgacacgac gtgttcgagc
     1081 ccgagcgcac agccctgcag cacggcgcgg aggttctgca tcacctggtg cgtctgtgcc
     1141 tcgatcgtgt cggggtacgc agatgtgttc gacgacgtcg tgaacggcat ctgcccggtc
     1201 aggaacaccc agccgtcggc ttccaccgcg tgactgaaag gcgcgacggg atcgggtgcg
     1261 ccgccgatca tgtggaacac gagtgcggac atcgaaagtg tctccgcgaa atggtgtcgc
     1321 tattctggtc ccgtcgccgc gccacggcga gtctcccgac cgatggccct tgccaggggc
     1381 cgcgcgccgc gtgctagact gcgccgcatg aggtctgaat tggttctcct cgcgtcgcaa
     1441 ccgccgctga ccggtgcctg gtcggcctac gtctattttt acttcggaca cgccgagggc
     1501 cgcacgcgac cgtagcaagc agacacacag gaatacatcg atcgggcggc tccaaaaggg
     1561 ccgcccgatt tgtttttgca agccccatca ctgaaggagt agacgatgat catcgtgatg
     1621 caacccgacg ccggcgatgc ggagatcgcc gccgtcgaag ccaagattcg cgcgcatgga
     1681 ctcgacgtcc atatttcccg cggcaccgag cgcacgctca taggcgcgat cggcgacgag
     1741 cgcaagctcg atcccgagat gttcgatccg ctcccgggcg tcgaacggtc gctgcacata
     1801 ctgaagccct acaagctcgt tgcgcgcgaa tggcacaagg cgagcacgat cgtcgacgta
     1861 tgcggtgcgc gcatcggcgg caacgaagtg caggtgatcg ccggaccatg ctcggtggag
     1921 acggtgccgc agatgcaggc ggcggcggcg ggcgtcgcgt cggccggtgc ccgactgatg
     1981 cgcggcgggg cgttcaagcc gcgcaccagc ccttacacct tccaaggcaa gggcgtcgag
     2041 gggttgacca tgctgaagga cgccgccgcg cgtcacgacc tgccgatcgt caccgagctg
     2101 atggacgtgc gcatgctcga cacgttcctc gagcacggcg tcgacgtgat ccagatcggc
     2161 acgcgcaaca tgcagaactt cgacttgctg aaggaagtcg gcaaggtgaa cgtgccggtg
     2221 atcctgaagc gtggcatgag tgccacgatc agcgaatggc tgatggctgc cgaatatgtc
     2281 gccgccggcg gcaatcaccg gatcatgctg tgcgagcgcg gcatccgcac gttcgagaca
     2341 gcgtatcgca acgtgctcga cgtcacggcg gtcgcggtat tgaagaagga gacccacctg
     2401 ccggtgatca tcgatccgtc gcatgcgggc ggcaaggcgt ggatggtgcc ggcgctgtcg
     2461 tgcgcggcca ttgccgcagg cgccgatggt ttgctgatcg aagtgcatcc ctgcccggcg
     2521 gaggcatggt gcgacgccga tcaggcgctg tcgatcgatg agttcgcgac gctgatgaag
     2581 cgtttgggcg cggtggcgga ggcgatggga aggtcgcttg cgccggcgcg cgaggttacg
     2641 cggaccgagc tcgcgacggc gtgatgttca gcgtgtgcga gccagcagca actgcggctg
     2701 gcgactatca gaccgagagc tcggtgatgg gaatgggcat tagggcctgt tcacactatg
     2761 tccacgcacg cgttgcagtg ccgaaatggc catgcgcgag
//
LOCUS       DASSVX010000127         8781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_488193, whole genome shotgun sequence.
ACCESSION   DASSVX010000127 DASSVX010000000
VERSION     DASSVX010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488193"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFO53_05510"
     CDS             <1..260
                     /locus_tag="VFO53_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875768.1"
                     /translation="GWVGLVLILLALVIVALSARTALKAYGLFDDSDKPRHAAAPGAN
                     ETEQAATITPRNAIERAKGVQDMVKQGAIEQEKRIDDAIPK"
     gene            325..996
                     /locus_tag="VFO53_05515"
     CDS             325..996
                     /locus_tag="VFO53_05515"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HET8875769.1"
                     /translation="MPVTGYPHPIIAREGWPFVAGAFVIALVLSLASWWALAALAWVA
                     FLFILQFFRDPPRIVPGAPNAVLSPADGRVVRIGNARDPYLDRDALLISVFMNVFNVH
                     SNRSPVDADVAQTWYHPGTFVNAALEKASSDNERNALRLVTPAGVEVTCVQIAGLIAR
                     RILCYVKAGDRLMRGQRYGFIRFGSRVDLYLPPDAIPRATVGDKVYATQSIVAELRPP
                     RALNP"
     gene            993..1772
                     /gene="pssA"
                     /locus_tag="VFO53_05520"
     CDS             993..1772
                     /gene="pssA"
                     /locus_tag="VFO53_05520"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HET8875770.1"
                     /translation="MIDDDDLQSERRLLKNRVRRRGIYLLPNLFTTAALFAGFYAIVQ
                     AMNVNFDQAAIAIFVAMVLDGLDGRVARMTRTQSAFGAEFDSLADMVSFGAAPALVMY
                     EWVLRDLGKLGWIAAFVYVAGAALRLARFNTLLEVADKRYFTGLPSPSAAALVAGFVW
                     VIDDLGYDPDALRWLAWAVTLFAGLTMVSNLKYYSFKTINLKRSVPFVAIFLIVLLVA
                     LLSWQPPLVLFAAFVAYGLSGYAVSAWMLARARRVRTPGPP"
     gene            1776..2156
                     /locus_tag="VFO53_05525"
     CDS             1776..2156
                     /locus_tag="VFO53_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET8875771.1"
                     /translation="MPGEPVPLRWFMLTLGCVALLAVGQVLFKVAALAWRVDGWSWAT
                     LRSFLSLPMLAALAIYAIATVAWVFVLRFVPLVAAYSIFSLAFVITPLFAHFALGEPL
                     ALRTLMGGALIVVGVVVASTASSG"
     gene            2153..3124
                     /locus_tag="VFO53_05530"
     CDS             2153..3124
                     /locus_tag="VFO53_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET8875772.1"
                     /translation="MIAVVIPCYRTRRQILDVLSRIDANVSRIYVVDDGCPEGTGDLV
                     EKSLVDDRVRVLRHERNLGVGAAVVTGYRAALADGARIVVKIDGDGQMDPAQMSRIVA
                     PVAMGAADYAKGNRFYDFALLQDMPRVRLLGNALLSLVNKISSGYWNVMDPTNGYTAI
                     HRIALEALPLAKIDSGYFFESDMLFRLYTVRAVVRDVPLRARYADESSNLRIGRVLRD
                     FPVKYVAATVKRIFYSYFLRDFNAGTLQLASGLLLLSLGGAYGVTKWINSSVSGFPTT
                     SGAVMLAALPVLVGVQLLLGALQFDIQNVPRTPLCAEPALAGDPQAG"
     gene            complement(3121..5259)
                     /locus_tag="VFO53_05535"
     CDS             complement(3121..5259)
                     /locus_tag="VFO53_05535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET8875773.1"
                     /translation="MRLAGFHDPTATASRWLMGALAVVLLVHAGVLAEWLSGLLQRPD
                     ATVLPGDEFRPVMLRASAVALLALLIFRRSPRRAASIVASAAACALVGVGPVATVALM
                     TLSAHVIGTALLDWSGSGAARATANAPASAAIRTLTGSCIWLGLIGATISLPIHDGAL
                     YVICAGAPLAIFPARTRMSVANALKWLAVDDVWSPSERLCLALAVVIAIMHLIVVAKP
                     EVGYDAIAMHLQFAELVGHAHRWDFDVTRYAWAVMPMGADYAYLAAYLVGGESAARLL
                     NLVFGLLLCALIYDIVRMQASRLIALVSVCLLASTPLWLLESSTLFVEFAWTAFLVAA
                     LASALRFYERREAACFTAAALCCAGAMACKVIGVFWVVPLAVALLYRLRLADRRAPAR
                     ARLVVVSLLAVVIASWPYVNAWVRTGNPVFPFMNTLFASPLFPAGEAFNNPVYNSPLS
                     LVTPYSLVMDSGRYLEGYDGAAGIQWLLLFPLIAFGLFTRSRRRRSALFCLAVGFAVP
                     VYVQQSYLRYLFPAFVLLAILAGWALGDWIRSPRSAAGLAVATGIVAVANLWLMPSAN
                     WANATLCIRCAQDADARRAYVARYAPLRIVGDYLNRNLPDARVGFFVLNAAGASGFVG
                     YSRAANWHDNPVFRDITAATTSDDVAAIVRRFRLTHAVFVDRSDNPFDRVLIEYRDRE
                     TTPVARIGNYVITAIRANDARAASAAAPPR"
     gene            5531..7249
                     /gene="leuA"
                     /locus_tag="VFO53_05540"
     CDS             5531..7249
                     /gene="leuA"
                     /locus_tag="VFO53_05540"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET8875774.1"
                     /translation="MHAAPSHKYRPFQPIDLPDRAWPSRTIVAAPIWLSTDLRDGNQA
                     LIEPMDAGRKLRLFRQLVDIGFKEIEVAFPSASQTDFDFVRQLIDENLIPDDVTIQVL
                     TQSRDELIRRTFESLRGAARAIVHLYNPTAPLFRRVVYGKDRAGIVEIATQGARLFAE
                     CAAQQPQTDWRFEYSPETFTMTELDFALEICERVVDVLEPVTRHPVILNLPATVEVAT
                     PNVFADQIEWMHRHLSRRDRVLISVHPHNDRGTGVAAAELAVMAGADRVEGCLFGNGE
                     RTGNVCVVTLAMNLYSQGIDPKLDFSDIGAVIRTAEYCTALPVHPRHPYAGELVFTAF
                     SGSHQDAIKKGFAAQRAAEAAGSTIWEVPYLPIDPADVGRTYEAVIRVNSQSGKGGIA
                     YLLERDYGLALPRLLSIEFSLVVQAIADATGKELTAADLHAAFQREYVSPQSPVAYVA
                     HRAAHSGVQGALEQLTARLAVDGVETLVAGAGNGPVDAFVHGLREGLGIDVHVLAYHE
                     HATGSGEDATAVAYVQLRAGTKSVYGVGLDPNIVTATLKAVTAAVNRACAQGALSLGT
                     PVGAAA"
     gene            7414..>8781
                     /locus_tag="VFO53_05545"
     CDS             7414..>8781
                     /locus_tag="VFO53_05545"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET8875775.1"
                     /translation="MSDHLIIFDTTLRDGEQSPGASMTKEEKTRIARQLERLRVDVIE
                     AGFPASSNGDFDAVHDIAKAIRESTVCALARANENDVRRAGEALRPAKSMRIHTFIAS
                     SPIHMQMKLRMTPEQVLEQAVKAVGWARRFTDDVEFSPEDAGRSEPDFLCRLLEAVIA
                     AGATTVNIPDTVGYTMPWQFGALIGSLRERVPNSDRAVWSVHCHNDLGVAVANSLAAV
                     MHGARQVECTVNGLGERAGNASLEEIVMAVRTRKDVFSCTTRIDATQIVPASKLVSGI
                     TGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVGWHANKLVLGKLSGR
                     NALRTRLVELGIDLPGDEAFNVAFARFKELADKKGEVFDEDLHALVSDEGILPAHEHY
                     RLAALKVTSETGKVPHARVVMAVGEAELVTEVDGDGPVDAVFRAIERMAGSHANLLLY
                     SVNAIT"
BASE COUNT         1444 a         2989 c         2940 g         1408 t
ORIGIN      
        1 gcggctgggt cggcctcgtg ctgatcctgc tggcgctcgt catcgtcgcc ctgtcggcgc
       61 ggacggcgct caaggcgtac ggcctgttcg acgattccga caagccgcgg catgcagccg
      121 cgccgggcgc gaacgagacc gagcaggcag cgacgatcac gccgcgcaat gcgatcgagc
      181 gcgcgaaagg cgtgcaggac atggtgaagc agggcgcgat cgagcaggaa aagcgcatcg
      241 acgacgcgat cccgaagtga cggcatgggc cgcggcatcc tgccaaccgg gcccgcgcag
      301 cgcgtgcgcc gcgcgtgatt gccgctgcca gtgaccggct atccgcatcc gatcatcgcg
      361 cgtgaaggct ggccgttcgt cgccggcgcc ttcgtcattg cgctcgtgct gtcgctcgcg
      421 tcgtggtggg cgctcgccgc gcttgcctgg gtcgcgttcc tgttcatcct gcagttcttc
      481 cgcgacccgc cgcgaatcgt gcctggcgcg ccgaatgccg tgctgtcgcc tgccgacgga
      541 cgcgtcgtgc gcatcggtaa cgcgcgcgat ccctacctcg atcgcgacgc gctgttgatc
      601 agcgtgttca tgaacgtgtt caacgtgcat tcgaaccgca gccccgtcga tgccgacgtc
      661 gcgcagacgt ggtatcaccc gggcacgttc gtgaacgccg cgctggagaa ggcgtcgagc
      721 gacaacgagc gcaacgcgtt gcggctcgtc acgccggcag gcgtcgaggt gacctgcgtg
      781 cagattgcgg gactgatcgc gcggcgcatc ctctgttacg tgaaagccgg cgaccggctg
      841 atgcgcggac agcgttacgg gttcatccgc ttcggctccc gcgtcgacct gtatctcccg
      901 cccgatgcga tcccgcgcgc caccgtcggc gacaaggtct atgcgacgca gtcgatcgtt
      961 gccgagttga ggcctccgcg agcccttaat ccatgatcga tgacgacgac ctgcaaagcg
     1021 agcggcggct gctgaagaac cgcgtgcgca ggcgcggcat ctacctgctg ccgaacctgt
     1081 tcacgacggc cgcgctgttc gccggcttct acgcgatcgt gcaggcgatg aacgtgaact
     1141 tcgaccaggc agccatcgcg atcttcgtcg cgatggtgct ggacggcctc gacggccggg
     1201 ttgcgcggat gacgcgcacg cagagcgcat tcggcgccga attcgacagc ctcgccgaca
     1261 tggtgagctt cggcgcggca ccggcgctgg tcatgtacga gtgggtgctg cgcgacctcg
     1321 gcaagctcgg ctggattgcc gcgttcgtct acgttgccgg cgccgcgctg cgtcttgcgc
     1381 gcttcaacac gctgctcgag gtcgccgaca agcgctattt cacgggcctg ccgagcccgt
     1441 cggctgcggc gctcgtcgcc ggtttcgtct gggtgatcga cgatctcggc tacgatccgg
     1501 acgcgctgcg ctggcttgcg tgggcggtga cgctgttcgc cggcctgacg atggtcagca
     1561 acctcaagta ctacagcttc aagacgatca acctcaagcg gagcgtgccg ttcgtggcga
     1621 tcttcctgat cgtcctgctc gtggcgctcc tgtcctggca gccgccactc gtgctgttcg
     1681 ccgcattcgt cgcctacgga ctgtccggct acgccgtgtc ggcgtggatg ctcgcgcgtg
     1741 cacggcgcgt gcgcactccc gggccgccat gaccgatgcc cggtgaacca gtgccgctgc
     1801 gctggttcat gctcacgctc ggctgcgttg cgctgcttgc ggtcggacag gtgctgttca
     1861 aggtggccgc gctcgcgtgg cgcgtcgacg gctggtcgtg ggcgacgctg cgaagcttcc
     1921 tgtcgctgcc gatgctggcg gcgctcgcca tctacgcgat cgcgacggtt gcctgggtct
     1981 tcgtgcttcg cttcgtcccg ctcgttgccg cctactcgat cttttcgctt gcgttcgtca
     2041 tcacgccgct cttcgcgcat ttcgcgctcg gcgagccgct cgcattgcgc acgctgatgg
     2101 gcggcgccct catcgtcgtc ggcgtcgtgg ttgcttccac cgcgtcgtcc ggatgatcgc
     2161 cgtcgtcatt ccgtgttatc gaacacgccg gcagatcctc gacgtcctgt cgcgaatcga
     2221 cgccaacgtc tcgcgaatct acgtcgtcga cgacggatgt ccggaaggca ccggcgatct
     2281 cgtcgaaaaa tcgctcgtgg atgaccgcgt gcgggtgctg cggcacgagc gcaatctcgg
     2341 tgtgggcgcg gctgtcgtga ccggctaccg ggccgcgctc gccgacggcg cacgaatcgt
     2401 cgtcaagatc gacggcgacg ggcagatgga cccggcacag atgagccgca tcgtcgcgcc
     2461 ggtcgccatg ggcgcggccg attacgcgaa gggcaatcgt ttctacgact tcgcgctgct
     2521 gcaggacatg ccgcgggtgc gcctgctcgg caacgccctc ctgtcgctcg tcaacaagat
     2581 ctcgagcggc tactggaacg tcatggaccc gaccaacggg tacacggcca ttcaccgcat
     2641 cgcgctcgaa gcgctgccgc ttgcgaaaat cgacagcggc tacttcttcg agtcggacat
     2701 gctctttcgc ctgtataccg tgcgcgccgt cgttcgcgac gtgccgctgc gcgcccgcta
     2761 cgccgacgag tcgagcaact tgcgcatcgg ccgcgtgctg cgcgatttcc cggtcaagta
     2821 cgtggcggca acggtcaagc gcatcttcta ttcgtacttc ctgcgcgatt tcaacgcggg
     2881 aacgctgcag ctcgcgtcgg gtctgctgct gctgtcgctc ggcggcgcat acggcgtgac
     2941 gaagtggatc aattcgtcgg tgtccggatt cccgacgacg tcgggtgccg tcatgctcgc
     3001 cgcactgccc gtgcttgtcg gcgtgcagct cctgctcggt gcgctccagt tcgacatcca
     3061 gaacgtgccg cgcacgccgc tttgcgccga accggcgctg gccggcgacc cgcaggcggg
     3121 ctaacgcggc ggcgctgccg ccgacgccgc gcgcgcatcg ttcgcgcgta tggccgtgat
     3181 cacgtagttc ccgatgcgcg ccaccggtgt cgtctcccgg tcgcggtact cgatcagcac
     3241 gcggtcgaac gggttatcgg agcgatcgac gaataccgcg tgggtgagcc ggaagcggcg
     3301 cacgatcgcc gccacgtcat cgctcgtggt cgccgcggtg atgtcgcgga acaccggatt
     3361 gtcgtgccag ttcgcggcgc gcgaatagcc gacgaagccg ctcgcgcccg ccgcattgag
     3421 aacgaagaat cccacccggg cgtccgggag attgcggttg agatagtcgc cgacgattcg
     3481 cagcggcgcg tagcgggcga catacgctcg ccgcgcgtcg gcgtcctgcg cgcaacggat
     3541 gcaaagcgtc gcgttcgccc agttcgccga tggcatcagc cacaggttcg cgaccgccac
     3601 gatgcccgtc gcaaccgcga gcccggccgc cgaccgcggc gagcgaatcc agtcgccaag
     3661 cgcccatccg gcgaggatcg caagcagcac gaacgccggg aacaggtagc gcaggtacga
     3721 ctgctgcacg tacaccggca cggcgaatcc caccgccagg caaaagagcg ccgaccggcg
     3781 ccggcgcgag cgggtgaaaa gaccgaaggc gatcagcgga aacagaagca gccattgaat
     3841 tccggccgcg ccatcgtagc cttcgagata gcggccgctg tccatgacca gcgaatacgg
     3901 cgtgacaagc gacagcggcg agttgtagac ggggttgttg aaagcctcgc cggccgggaa
     3961 cagcggcgac gcgaacagcg tgttcatgaa cgggaacacg ggattgcccg tgcgcaccca
     4021 cgcattcacg tacggccatg aagcgatgac gacagcgagc agcgacacga caaccagccg
     4081 ggcgcgcgcc ggcgcacgcc ggtcagcgag tcggagtcga taaagcagcg caaccgcaag
     4141 cggcacgacc cagaacacgc cgatcacctt gcacgccatc gcgcccgcgc agcacagcgc
     4201 tgcagccgtg aagcacgccg cttcgcgccg ctcgtagaag cgcaaggccg acgcgagcgc
     4261 cgcgacgagg aacgccgtcc atgcgaactc gacgaacagc gtcgacgact cgagcagcca
     4321 gagcggcgtc gacgcaagca ggcaaacgct caccagcgcg atcaggcggc tcgcctgcat
     4381 gcgcacgatg tcgtagatca gcgcgcagag cagcaggccg aagaccaggt tgagcagtcg
     4441 cgcggccgat tcgccgccga ccaggtaggc ggccaggtac gcgtagtcgg cgcccatcgg
     4501 catcaccgcc catgcgtacc gggtcacgtc gaaatcccag cgatgggcgt ggccgacgag
     4561 ctcggcgaac tgcaggtgca ttgcgattgc gtcgtagccg acctccggct tcgcgacgac
     4621 gatcagatgc atgatcgcga tgacgacggc gagggcgagg cacagtcgct cggagggtga
     4681 ccagacgtcg tccacggcca gccacttcag tgcgttcgcg accgacatcc gtgtcctggc
     4741 gggaaatatc gccagcggcg cgccggcgca gatcacgtac agcgcgccgt cgtgaatcgg
     4801 cagcgaaatg gtggcgccga tcaaccccag ccagatgcaa ctgcccgtca gcgtgcgaat
     4861 ggccgcggac gcgggcgcat tcgctgttgc gcgggccgcg ccgctgccac tccagtcgag
     4921 cagcgccgtc ccgatcacgt gcgcggacag cgtcatcaac gcgaccgttg cgaccggtcc
     4981 gacacccacc agcgcgcacg ccgccgcact ggccacgatg ctcgccgcac gcctcggtga
     5041 ccggcggaag atcagcagtg cgagcagcgc gaccgccgac gcccgcagca tcaccggccg
     5101 aaactcgtcg ccgggcagga cggtggcatc cggccgctga agcaatccgg aaagccactc
     5161 ggcgagcacg ccggcatgca ccagcagcac gaccgccagc gcgcccatca gccagcgcga
     5221 ggcggtcgca gtcgggtcgt ggaatccggc gaggcgcatg gaccagcggc aaaaacggac
     5281 gcaagtgtcg cacggccggg gcactcaggc ggggcaacgg ttgcatgcaa cggccatttc
     5341 ggctaacgta gcacgatgcg cagcctctcc cccgccgtcc gcgccctcgc gccagccctc
     5401 cttccgggcg gcctgctgct gcttctgctg cgccccgcgc gcagataaaa acccacccct
     5461 cgctcgaaag ccgtgcccgc accatccgaa aggatgggcg atcttcattc ttcgttcccg
     5521 ggattccgcc atgcacgccg cgccctcgca caagtaccgt ccgtttcagc cgatcgatct
     5581 tccggatcgc gcctggccct cgcgcacgat cgtagccgcg ccgatctggc tgtcgaccga
     5641 tctgcgcgac ggcaaccagg cgctgatcga gccgatggac gcgggtcgca agctgcgcct
     5701 gttccgccag ctcgtcgaca tcggcttcaa ggagatcgag gtcgcgtttc cgtccgcgtc
     5761 gcaaaccgac ttcgatttcg tccgccagtt gatcgacgaa aatctcatcc ccgacgacgt
     5821 cacgatccag gtgctgacgc aatcgcgcga cgagctgatt cggcgcacgt tcgagtcgct
     5881 gcgcggcgct gcgcgcgcga tcgtgcatct ctacaatccg acggcgccgc tgttccggcg
     5941 cgtcgtgtat ggaaaggatc gtgcggggat cgtcgaaatc gccacccaag gcgcgcgcct
     6001 tttcgccgag tgcgcagcgc agcagccgca aaccgactgg cgcttcgaat actcgccgga
     6061 aacgttcacg atgaccgagc tcgattttgc gctcgagatc tgcgagcgcg tcgtcgacgt
     6121 ccttgaaccg gtgacgcgcc acccggtgat cctcaacctg cctgcgaccg tcgaggtcgc
     6181 gacgcccaat gtgttcgccg accagatcga gtggatgcac cggcaccttt cgcgccgtga
     6241 ccgcgtgctg atctcggttc acccgcacaa tgatcgcggc accggcgtcg cggccgccga
     6301 gctcgcggtc atggcgggcg ccgaccgcgt cgagggctgc ctcttcggca acggcgagcg
     6361 caccggcaac gtctgcgtcg tcacgctcgc gatgaacctg tattcgcagg ggatcgaccc
     6421 gaagctcgac ttcagcgaca tcggcgctgt gatccgcacg gcggaatact gcacggcgct
     6481 gccggtccat ccgcgccatc cgtacgcggg cgagctcgtg ttcaccgcgt tttccggctc
     6541 gcatcaggac gccatcaaga agggcttcgc cgcgcagcgc gccgccgaag cggcgggaag
     6601 cacgatctgg gaggtgccgt acctgccgat cgatccggcc gacgtcgggc gcacgtacga
     6661 ggcggtgatc cgcgtcaaca gccagtcggg caagggtggc atcgcgtacc tgctcgagcg
     6721 cgactacggg ctcgcactgc cgcggcttct ttcgatcgag ttcagcctgg tggtgcaggc
     6781 gatcgccgac gcgaccggca aggagctgac ggcggccgat ctccacgcgg cgtttcagcg
     6841 cgaatacgtg tcgccgcaat cgccggtcgc gtatgtcgct catcgtgccg cgcattcggg
     6901 cgtccagggc gcgctcgagc agctcacggc ccgcctggcc gtcgacggcg tcgaaacgct
     6961 ggtggcgggt gcgggcaacg gcccggtcga tgcgttcgtg cacggcctgc gcgaaggcct
     7021 cggcatcgac gtgcacgtgc tcgcctatca cgagcatgcg acgggcagcg gcgaggacgc
     7081 caccgccgtc gcgtatgtgc agcttcgtgc gggaacgaag agcgtctacg gcgtcgggct
     7141 cgatccgaac atcgtcaccg cgacgctgaa ggccgtcacc gcggcagtca atcgcgcctg
     7201 cgcacagggt gcgctgtcgc tcggcacgcc ggtgggcgcc gccgcatgag cgaccgacgg
     7261 agcatcatga gcggcgaagg gccgtcccga gccgcgaatt ccgccccctg gggggcagcg
     7321 tagcgcgcga gcccgacggg ccgccccgag ggcgagcttc cgagcgcaaa gcgcgaaggc
     7381 cgttcgctcg gccgcaagcg tggggggatg tttatgtccg atcatctgat catcttcgat
     7441 acgacgttgc gcgacggcga gcaaagcccg ggcgcctcga tgacgaagga ggaaaagacg
     7501 cgcattgcgc gccagctcga gcggcttcgc gtcgacgtga tcgaggccgg cttccccgcg
     7561 tcgtccaacg gcgatttcga cgcggtgcac gacatcgcga aggcgatccg cgaaagcacg
     7621 gtatgcgcgc tcgcacgcgc gaacgaaaac gacgtgcggc gcgccggcga ggcgcttcgg
     7681 ccggcgaaat cgatgcgcat ccatacgttc atcgcgagct cgccgatcca catgcagatg
     7741 aagctgcgga tgacgcctga gcaggtgctc gagcaggcgg tcaaggcggt gggctgggcg
     7801 cgccggttca ccgacgacgt cgagttctcg cccgaggacg cgggccggtc ggagcccgac
     7861 ttcctgtgcc ggctgctgga agcggtgatc gcagcgggcg ccaccacggt caacatcccc
     7921 gacaccgtcg gctacacgat gccatggcaa ttcggcgcgc tgatcgggtc gttgcgcgag
     7981 cgcgtgccga attcggaccg cgcggtgtgg tcggtgcact gccataacga cctcggcgtc
     8041 gccgtcgcga actcgctcgc cgcggtgatg cacggtgcgc ggcaggtcga atgcacggtg
     8101 aacggcctcg gcgagcgcgc cggcaacgca tcgctcgagg agatcgtgat ggcggtgcgg
     8161 acgcgcaagg acgtgttttc gtgcacgacg cgtatcgacg ccacgcagat cgttccggcg
     8221 tcgaagctcg tatccggcat cacgggcttt ccggtgcaac cgaacaaggc gatcgtcggc
     8281 gccaacgcat tcgcgcacga gtcgggcatc catcaggacg gcgtgctgaa gcatcgcgag
     8341 acgtacgaga tcatgcgcgc cgaggatgtc ggctggcatg cgaacaagct cgtcctcggc
     8401 aagctttccg gtcgcaacgc gttgcgcacc cgtctcgtcg agctcggcat cgatctgccc
     8461 ggcgacgagg cgttcaacgt cgcgttcgcg cgtttcaagg agctcgccga caagaagggc
     8521 gaagtgttcg acgaggacct gcacgcgctc gtctccgacg aaggcatcct cccggcgcac
     8581 gagcattacc ggctggctgc gctcaaggtc acgtcggaga ccggcaaggt gccgcatgcg
     8641 cgcgtcgtga tggcggtcgg cgaggcggag ctcgtgaccg aggtcgatgg cgacggcccc
     8701 gtcgacgcgg tgttccgcgc gatcgagcgc atggcgggat cgcatgcgaa cctgctgctc
     8761 tattcggtga acgcgatcac c
//
LOCUS       DASSVX010000128         9545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_154817, whole genome shotgun sequence.
ACCESSION   DASSVX010000128 DASSVX010000000
VERSION     DASSVX010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9545
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154817"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFO53_05550"
     CDS             <1..447
                     /locus_tag="VFO53_05550"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HET8875776.1"
                     /translation="PHWEHAGDAASIAIFHGPHAYVSPSWYAEPSKAVPTWNYAAVHV
                     HGTLEVIRDPLETRRVLDALVQRFEATRDAPWTFAMPERQRDALVSAIVAFRVRVRSL
                     EAKFKLSQNRPRHDQVRVAAALDGEGYADATAVAAWMRAYGGTAQE"
     gene            444..857
                     /locus_tag="VFO53_05555"
     CDS             444..857
                     /locus_tag="VFO53_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET8875777.1"
                     /translation="MTRGIGGAPEAMREGRVRIDKWLWAARFYRTRSLAAEAVEAGHV
                     RVDGERVKPAHPLRPASRVTIRKRELQWDVDVLGLSERRGSAPEAALLYRETAESAGA
                     RERLLAERAKARAASTTGRPTKRDRRKLEDFLDEP"
     gene            complement(859..1035)
                     /locus_tag="VFO53_05560"
     CDS             complement(859..1035)
                     /locus_tag="VFO53_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875778.1"
                     /translation="MDSRDASLPPGPVTPTGGARRLARHALAMLLAAALAWLVFAAYG
                     QPGFILDFAGMRLC"
     gene            complement(1046..2071)
                     /locus_tag="VFO53_05565"
     CDS             complement(1046..2071)
                     /locus_tag="VFO53_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET8875779.1"
                     /translation="MGILIEAPSRRQDPSGAPANAPAVAMGEPAGALHIREVVRITGL
                     RREQLYMWQRRYGFPSPLRDAFGDRVYPAEQVARLKLIKQLLSEGWRAGAVVPLADTA
                     LQSMVGLAVAEPAPLPADIAHALRLLGEHRIGELTNHLSKLLVAQGLRKFLEHTLIPL
                     NEAVHERVVRGEMQTYQELRFCDLALRLLRDVTRLARPTRDARQLLLATPPNDPNQLG
                     LAILELLLFTEGVNCLTLGTGVPGQEIAGAAEAYHASIVVLLFDRGISGKIAGQEIRS
                     LRRSLAAAVPLIVSGRAVNLLAKPIENVQVAADFNSVMAALRARGALPAAPVTRVPDL
                     PDTSQTA"
     gene            2407..4164
                     /locus_tag="VFO53_05570"
     CDS             2407..4164
                     /locus_tag="VFO53_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875780.1"
                     /translation="MFGFGRSTKDPLSDVRSATRWLATLPGADSLAVHTEVVKELDRV
                     ADVPAALTLNRMRAVFHVDAQTGAQRRALVAQYVEHASRSSRIENQLWTALFRLTQSF
                     LAIYQALAREIAERPQSARWQGLMPELLCRQIVHLALDARVRLFRYEQWIPAKWAELH
                     GLMTLACSRQCERQIVTLADAATTTIEHEYLRALVLQLMSAGSLTARQVEFIWGELDD
                     WCAPLRLTLEPSAANSFFVDLAGREGLRRRKIGPLESRVLFLDTRPLHAVMMQHVVTL
                     EQKIRAEPLSNRTPRRSEQLTLFAKLAAQVDPEFKPFARRGERVATSGNVDAIVGFQK
                     ITSYFREDARQPIDLADSTGESFDSTMDIAVFGRIRDEPTRRREIARRRIGAHAAPGG
                     PWEAKDVSQTGFKLVAPIQVANAVTLGTLVAIHPHGQPRWTLGIIRRMKRTSADRAEI
                     GLAIVADTICAVDVNEQRKRTAEEYSIDGEGTTINGRRFGGLLLSLRPRDGEPMVQSL
                     IVPAVEYQAAKRYQVATEKAEYRIRFGRLIEQQPDWVWTAIEPLDGSARASAPATGGP
                     TTVTGMTTVTGTSVSQSRG"
     gene            4239..5906
                     /locus_tag="VFO53_05575"
     CDS             4239..5906
                     /locus_tag="VFO53_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET8875781.1"
                     /translation="MATLDRYAEIHAGYRWEVPADFNIAHACCTRWAAERSRFALYWE
                     DEDGTRAAYTFFDLAQQANRLANALAARGIARGDKVAIVLPQRPETAVAHIAVHQLGA
                     VSVPLSFLFGPDALEYRLLHSGAKAVLVDPRSLPNLLPVRERCAALAHVIGVAGATAP
                     GIVAYETLLDESSRHFTPVATRADDPAILIYTSGTTGPPKGALMPHRCLIGNLPGFTH
                     SHDGYPQPGDLFWSPADWAWTGGLMDALLPAFYFGQPIVGYRGRFDPERAFRLIERYQ
                     IRNTFLFPTALKLMMKAYPRPRETFDVNLRSIMSAGEAVGTAVFDWARDALGVTINEM
                     FGQTEMNYIVGNSHALWPAKPGSMGRPYPGHRIAIIDDEGNEVSRGEPGDVAIHRHAP
                     DGTRDPVFFLEYFGNPEGTRNKYTGDWCRTGDIAVCDDDGYLWYQGRADDMFKAAGYR
                     IGPSEIENCLVRHPAVGNAAVVPSPDDVRGNIVKAFIVLAPGYVASAALEADIARHVR
                     EHLAPYEYPKEIEFIDALPMTTTGKVQRKVLRERELARKRAVNLPTQ"
     gene            complement(5931..6530)
                     /locus_tag="VFO53_05580"
     CDS             complement(5931..6530)
                     /locus_tag="VFO53_05580"
                     /inference="COORDINATES: protein motif:HMM:NF041730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H-associated membrane protein"
                     /protein_id="HET8875782.1"
                     /translation="MHAEVAEAQKRAKSSVARFALRTLAFLPLAFALWYFAAPLLLWP
                     ALQLVRAVGYAAFGDIVRGVEQSGALATFVTALHAPGAATTGIITVDVNLLLYSFGLP
                     LFVALTLAARERRWKRHLVVGYAALQPIVAWGVLADFLKNVAITAGPAVASQTGFVAW
                     QREAIAFAFQLGSLILPAVVPAVLWVALHGRFIPALRRP"
     gene            complement(6569..7078)
                     /gene="xrtH"
                     /locus_tag="VFO53_05585"
     CDS             complement(6569..7078)
                     /gene="xrtH"
                     /locus_tag="VFO53_05585"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HET8875783.1"
                     /translation="MRRFLAVFVVLLVLSFAIELTPVAQAWFVAPWTDAVASASAALM
                     RVFDTTIATSGNVISSSASPFAVSIEAGCNGVEATLVLVAAMLAFAAPWPHRLRGIVV
                     GIVAVQALNVLRVISLFYLGRWNRDVFEWAHLYVWQALIMLDVLIVWVIWVRLVPASP
                     RAALPVGTA"
     gene            complement(7134..8426)
                     /locus_tag="VFO53_05590"
     CDS             complement(7134..8426)
                     /locus_tag="VFO53_05590"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET8875784.1"
                     /translation="MPERARRRPGALRLVALERRRALRWLVLPAAQWLMPSAWADASR
                     IASARVWPAQEYTRVIFESSSPIEHQLVVLRDPNRVVLDLARIVPSRELDELASHLQR
                     TDPYIEAIRIGRPSDGFLRVVLDLKTEVRPQVFSLKPVAEYGHRLVLDLYPLTPVDPL
                     MALLQRDGFDARADTAPPRANASTRPDARKEPHDNLASRRITIALDPGHGGEDPGAIG
                     RRGTYEKNVVLAIGRRLQHVLDADTNMRVMLTRNDDFYVPLAERVRKARRVQADLFVS
                     IHADAFRESSARGSSVFALSEHGATSAAARWLAQKENAADLIGGVNLDVKDPVLARTL
                     LDLSQTAQISDSLKVGQEVLEGIGTHNALHKGSVEQAGFAVLKAPDIPSILIETAFIS
                     NPDEELKLRNPREQQRFAESIAEGIHRYFAQSPPLARA"
     gene            complement(8390..8890)
                     /gene="tsaE"
                     /locus_tag="VFO53_05595"
     CDS             complement(8390..8890)
                     /gene="tsaE"
                     /locus_tag="VFO53_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET8875785.1"
                     /translation="MRRNRDPVSTLTLHLPDAAATERAGANIAPILRGGMVVTLQGDL
                     GTGKTTLARGLLRARGVAGPIKSPTYALVEHYPVSSLYFYHFDFYRFMNPDEWDYAGM
                     SDYFRDDAVCIVEWPERVASRLPSPDLALSLSHPVHADGRALVVQAFSRAGEQCLSAL
                     AAGPAR"
     gene            8860..9420
                     /gene="queG"
                     /locus_tag="VFO53_05600"
                     /pseudo
     CDS             8860..9420
                     /gene="queG"
                     /locus_tag="VFO53_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253113.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
BASE COUNT         1611 a         3273 c         3251 g         1410 t
ORIGIN      
        1 ccgcactggg agcacgctgg ggatgcggcg tcgatcgcga tcttccacgg gccgcacgcc
       61 tatgtgtcgc cgtcgtggta cgccgagccc tcgaaagcgg tcccgacgtg gaactacgcg
      121 gccgtccacg tgcacgggac gctcgaggtc atccgcgatc cgctcgaaac gcgcagggtg
      181 ctcgacgcgc tcgtgcagcg cttcgaagcg acgcgcgatg ccccgtggac gtttgcgatg
      241 ccggagcggc agcgcgacgc gctcgtcagc gcgatcgtcg ccttccgcgt gcgcgttcgc
      301 agcctcgagg cgaagttcaa gctgtcgcag aatcgcccgc gccacgatca ggtccgcgtc
      361 gccgccgcgc tcgacggcga aggttacgcc gacgcgaccg cggtggcggc gtggatgcgc
      421 gcgtacggag gcacagccca ggaatgacgc gcggcatcgg cggcgcaccc gaagcgatgc
      481 gcgaaggccg tgtgcgcatc gacaaatggc tgtgggccgc gcggttctac cgtacacgca
      541 gcctcgccgc cgaagcggtc gaggccggtc acgtgcgcgt cgacggcgag cgcgtgaagc
      601 ctgcgcatcc actgcgcccg gcatcgcgcg tcacgattcg caagcgcgag ctccagtggg
      661 acgtcgatgt gctgggcctc tccgagcggc ggggttctgc accggaggcc gcgctgctgt
      721 accgcgaaac ggccgaaagc gccggggcac gcgagcgact gctcgccgaa cgcgcgaagg
      781 cgcgcgcggc cagcacgacg ggccgtccca ccaagcgcga ccggcgcaag ctcgaggact
      841 ttctcgacga accctagctc agcagagccg catccccgcg aaatcgagga tgaagccggg
      901 ttggccgtac gcggcgaaca cgagccaggc aagtgctgcg gccagcagca tggcgagcgc
      961 atgtcgcgcg aggcggcgcg cgccgccggt tggcgtgacc ggtccgggcg gcagggaggc
     1021 gtcgcgtgaa tccacgcgac cgaatttacg ccgtttgcga agtatcgggc aagtcgggga
     1081 cgcgcgtcac cggtgccgcg ggcagcgcgc cgcgtgcgcg cagcgccgcc atcaccgagt
     1141 tgaagtcggc agcgacctgc acgttctcga tcggcttcgc gagcagattc accgcccggc
     1201 cgctcacgat cagcggcacc gccgcggcaa gcgagcggcg aagcgaccgg atttcctggc
     1261 cggcgatctt gcccgaaatg ccgcgatcga acagcagcac gacaatcgac gcgtgatacg
     1321 cttcggcggc acccgcgatc tcctgccccg ggacgcccgt tccgagcgtc aggcaattca
     1381 cgccttcggt gaacagcagc aactcgagga ttgcgaggcc gagctggttc ggatcgttgg
     1441 gcggggtcgc gagcaacaat tggcgcgcgt cgcgcgtcgg ccgtgcgagg cgcgtgacgt
     1501 cgcgcagcag ccgcaacgcg agatcgcaga agcgcaattc ctggtaggtc tgcatttcgc
     1561 cgcggacgac gcgctcgtgc acggcttcat tgagcggaat cagcgtgtgc tcgagaaact
     1621 tgcgcaggcc ctgcgcgacc aggagcttcg acagatgatt cgtgagctcg ccgattcgat
     1681 gttcgccaag caatcgcagc gcgtgcgcga tgtcggcggg caacggcgcg ggttcggcga
     1741 cggccagccc caccatcgac tgcagcgccg tgtcggcaag cggaacgacc gcgcctgcgc
     1801 gccagccttc cgacagcagt tgcttgatga gcttgagccg cgcaacctgc tcggcgggat
     1861 aaacgcgatc gccgaaggca tcgcgcaacg gcgacgggaa gccgtaacgc cgctgccaca
     1921 tgtacagctg ctcgcggcgc agaccggtga tgcgaaccac ctcgcgaatg tgcagcgcgc
     1981 cggcgggctc gcccattgca accgccggcg cattcgccgg tgccccgctc ggatcctgtc
     2041 gcctcgaagg cgcctcgatg aggattccca tgtcgtgctt ccctggctgt tcgccccgct
     2101 ctcccgcatg ccacgcaaag cgcatgcacg gcggagctcc gcaacacgcc acacgcagcg
     2161 aacgtcatcg tcgacgtgct ggctcgtgct gcacggacag cggctatgca ggatgtgtgc
     2221 cacgtgccgc cggggacgcc gttgaatctt tcgcgtcctc gggccaattc ggtccgaaac
     2281 ttgcaggttg tgccgttcaa aaaagccggg gaccgtcgaa cggcccgcgt tcactggcaa
     2341 gagactcatt gaccgaggcg acgcgagtgc cctttcactg ctggacctga cgctccacga
     2401 cgcaagatgt tcggatttgg gagatcgacc aaggacccgc tgtccgacgt gaggtcggcg
     2461 acacggtggc tggccacgct accgggcgca gactcgctcg ccgttcacac ggaggtggtc
     2521 aaggagctcg accgcgttgc ggacgttcct gccgcgctca cgctcaatcg gatgcgggcg
     2581 gtgtttcacg tcgatgcaca gaccggtgca cagcggcgcg cgctggtcgc ccaatacgtc
     2641 gagcatgcca gccgcagctc gcgcatcgaa aaccagctct ggacggcgct cttccggctg
     2701 acgcaaagct tcctcgccat ctatcaggcg ctcgcgcgcg aaatcgccga gcggccgcaa
     2761 agcgcgcgct ggcaggggct gatgcccgag ctcctctgcc gccagatcgt gcacctggca
     2821 ctcgacgcgc gcgtgcggct ctttcgctac gagcagtgga tacctgcgaa gtgggccgag
     2881 ctgcatgggc tcatgacgct cgcgtgctcg cgacagtgcg agcggcagat cgtcacgctc
     2941 gccgatgccg cgacgacgac gatcgagcac gaatacctgc gggcgctcgt gctgcagctc
     3001 atgagtgccg gaagcctgac ggcacgccag gtcgaattca tctggggcga gctcgacgac
     3061 tggtgcgcgc cgctgcggct gacactcgag ccgtcggcgg cgaacagctt cttcgtcgat
     3121 ctcgccgggc gcgaaggatt gcgccgccgc aagatcggcc cgctggagag ccgcgtgctg
     3181 ttcctcgaca cgcgtccgct gcacgcggtg atgatgcagc acgtcgtcac gctcgagcag
     3241 aagatccgtg ccgagccgct gtccaaccgc acgccgcgac gcagcgagca gctgacgctg
     3301 ttcgcgaagc tcgccgcgca ggtcgatccc gagttcaagc cgttcgcccg ccgcggcgag
     3361 cgggtcgcaa cgagcggcaa cgtcgacgcg atcgtcggct tccagaagat cacgagctat
     3421 ttcagggaag acgcgcgcca gccgatcgac ctcgccgatt cgaccggcga gagcttcgac
     3481 agcacgatgg acatcgccgt gttcgggcgc attcgcgacg agccgacgcg ccggcgcgag
     3541 attgcgcgcc ggcgaatcgg tgcgcatgcc gcgcccggcg ggccgtggga agcgaaggac
     3601 gtatcgcaaa ccggcttcaa gctcgtggcg ccgatccagg ttgccaacgc cgtcacgctc
     3661 ggcacgctcg tcgccattca tccgcacggg caaccgcgct ggacgctcgg catcatccgc
     3721 aggatgaagc gcacgtcggc cgatcgcgcc gagatcggcc tcgccatcgt tgccgacacc
     3781 atttgcgccg tcgacgtgaa cgagcagcgc aagcgcaccg ccgaggaata ctcgatcgac
     3841 ggcgaaggca cgacgatcaa cggccggcgc ttcggtggct tgctgctgtc gctgcgcccg
     3901 cgtgacggcg agccgatggt gcagtcgctg atcgtgccgg cagtcgagta ccaggccgcg
     3961 aagcgctatc aggtcgcaac cgagaaggcc gagtaccgga tccgtttcgg ccgcctgatc
     4021 gagcagcagc ccgactgggt gtggacggcg atcgagccgc tcgacggtag cgcgcgcgcg
     4081 tcggcgccgg ccacgggcgg gccgacgacg gtgaccggca tgacgacggt gaccggcacc
     4141 agcgtgagcc aatcgcgcgg ctgacatgat ccggcggctt cgccgctgcg ctgcccggcg
     4201 ggactgcacg cacgacccac gctggttata ctcgcgcgat ggcgaccctc gatcgctacg
     4261 ccgagatcca cgccgggtac cggtgggagg tcccagccga cttcaacatc gcgcacgcgt
     4321 gctgcacgcg ctgggcggct gagcgttccc gcttcgcgct gtactgggaa gacgaagacg
     4381 gcacgcgcgc ggcgtacacg ttcttcgacc tcgcacagca ggcgaaccgg ctcgcgaatg
     4441 cgcttgccgc ccgcggcatc gcgcgcggcg acaaggtcgc catcgtcctg ccgcagcgcc
     4501 ccgagacggc cgtcgcgcac atcgccgtgc atcagcttgg cgcggtgtcc gtgccgctgt
     4561 cgttcctgtt cggacccgac gcgctcgaat accggcttct tcacagcggc gcgaaggcgg
     4621 tgctcgtcga tccgcggtcg ctgccgaacc tgcttcccgt gcgcgagcgc tgtgcagcac
     4681 tcgcgcacgt gatcggcgtc gcgggcgcca cggcgccggg catcgtcgcg tacgaaacgc
     4741 tgctcgacga atcgtcgcgc cacttcacgc cggttgcgac gcgcgccgac gatccggcga
     4801 tcctgatcta cacgagcggc acgacgggac cgccgaaggg cgcgttgatg ccgcaccgct
     4861 gcctgatcgg caacctgccg ggcttcacgc actcgcacga cggctatccg cagccgggtg
     4921 accttttctg gtctccggcg gactgggcgt ggacgggcgg tctcatggat gcgcttctgc
     4981 ccgcattcta tttcggccag ccgatcgtcg gttaccgtgg gcgtttcgat ccggagcgcg
     5041 ccttccggct gatcgagcgc taccagatcc gcaacacctt cctgtttccc accgcgctga
     5101 agctcatgat gaaggcgtat ccacgtccgc gcgagacttt cgacgtcaac ctgcgcagca
     5161 tcatgagcgc gggcgaggcg gtcggcacgg cggtcttcga ctgggcgcgc gacgcgctcg
     5221 gcgtgacgat caacgaaatg ttcggccaga cggaaatgaa ctacatcgtc ggcaactcgc
     5281 atgcgctgtg gcccgcgaag ccggggtcga tgggccggcc ctatccggga catcggatcg
     5341 cgatcatcga cgacgaaggc aacgaagtgt cacgcggcga gccgggtgac gtcgccatcc
     5401 atcgccatgc acccgacgga acgcgcgatc cggtgttctt cctcgagtac ttcggcaatc
     5461 ctgaaggcac gcgaaacaag tacacgggcg actggtgccg caccggcgac atcgccgtct
     5521 gcgatgacga cggctatctg tggtaccagg gccgcgccga cgacatgttc aaggcggccg
     5581 gctaccgcat cggcccctcc gagatcgaga actgcctcgt tcgccatccg gccgtcggca
     5641 atgcggccgt cgtgccgtcg cccgacgacg tgcgcggcaa catcgtcaag gcgttcatcg
     5701 tgctggcgcc ggggtacgtg gcatcggccg cgctcgaagc ggacatcgcg cggcacgtgc
     5761 gcgagcacct tgcgccgtat gagtatccga aggaaatcga attcatcgac gcgttgccga
     5821 tgacgaccac cggcaaggtg cagcgaaagg tgctgcgcga gcgggagctg gcgcgcaagc
     5881 gcgcggtcaa cctgccgacg caatgacgcg gcgccgcgcg ccgccccgcg tcatggccgg
     5941 cgcaacgccg ggatgaagcg gccgtgcagc gcgacccaca gcaccgcggg caccacggcg
     6001 ggcaggatca gcgagcccag ctggaacgcg aatgcgatcg cctcgcgttg ccatgcgaca
     6061 aaaccggtct gcgacgcgac cgccggcccg gcggtgatcg cgacgttctt caggaaatcg
     6121 gcgagcacgc cccaggcgac gatcggctgc agagcggcgt agccgacgac gagatggcgc
     6181 ttccacctgc gctcccgcgc ggcgagtgtc agcgcgacga acagcggaag gccgaacgaa
     6241 tagagcagca gattgacgtc gacggtgatg atgcccgtcg tcgccgcacc cggcgcatgc
     6301 agcgcggtca cgaacgtcgc gagcgcgccc gactgctcga cgccacgcac gatgtcgccg
     6361 aaggcggcgt atccgaccgc gcgcaccagc tgcaacgcgg gccacagcag cagcggcgcg
     6421 gcgaagtacc acagcgcgaa cgcgagcggc aggaaggcga gcgttcggag cgcgaagcgc
     6481 gcgacactcg atttcgcgcg cttctgcgcc tcggcgactt ctgcgtgcat cggtttgcgg
     6541 cggccgccgg cgatcgatcc gaatcggatc aggccgttcc gaccggcaac gccgctcgcg
     6601 gcgatgccgg cacgaggcga acccagatca cccacacgat cagcacgtcg agcatgatca
     6661 gtgcctgcca gacgtacagg tgcgcccact cgaacacgtc gcgattccac cgcccgaggt
     6721 agaagaggct gatcacgcgc agcacgttga gcgcctgcac cgcgacgatg ccgacgacga
     6781 tcccgcgcaa ccgatgcggc cacggcgcgg cgaacgcgag catcgcggca acgagcacca
     6841 gcgtggcctc gacgccgttg catccggcct cgatcgacac ggcgaacggg ctcgcggagg
     6901 aactgatcac gttgccgctc gtcgcgatgg tcgtgtcgaa cacgcgcatc aatgccgccg
     6961 acgccgacgc gaccgcgtcg gtccacggcg cgacgaacca cgcttgcgcg acgggcgtca
     7021 actcgatggc gaacgagagg acgagcagca cgacgaacac ggccaggaac cttcgcatcg
     7081 cccgcattct agatcgggtg tcgaaatggc ccttcgcggg ccgatgaacg ccctcatgcc
     7141 cgcgccagcg gcgggctctg cgcaaaatag cggtggatgc cttcggcgat cgattccgcg
     7201 aagcgctgct gctcgcgcgg gttgcgcagc ttcagctcct cgtcggggtt cgagatgaac
     7261 gccgtctcga tcaggatcga cggaatgtcg ggtgccttca gcacggcgaa tccggcctgc
     7321 tcgacgctgc ccttgtgcag cgcgttgtgc gtcccgatgc cttcgaggac ttcctgcccc
     7381 accttcaggc tgtcgctgat ctgcgcggtc tgcgacaggt cgagcagcgt gcgtgcgagc
     7441 accggatcct tgacgtcgag gttgacgcca ccgatcaggt cggcggcgtt ctccttttgc
     7501 gcgagccagc gcgcggccgc gctcgtcgcg ccatgctcgg acagcgcgaa aaccgacgag
     7561 ccgcgcgccg acgactcgcg aaacgcgtcc gcgtggatcg acacgaacag atcggcctgc
     7621 acgcgtcgcg ccttgcgcac gcgctcggcc agcggaacgt agaagtcgtc gttgcgcgtc
     7681 agcatcacgc gcatgttggt gtcggcgtcg agcacatgct gcaacctgcg cccgatcgcg
     7741 agcacgacgt tcttctcgta cgtgccacgg cgtccgatgg cgcccgggtc ctcgcctccg
     7801 tgtcccgggt cgagcgcgat cgtgatgcgg cgcgacgcga gattgtcgtg cggctccttg
     7861 cgtgcgtcgg gccgcgtcga tgcgttggcc cgtggtggcg cggtgtccgc gcgcgcgtcg
     7921 aagccgtcgc gctgcagcaa cgccatcagc ggatcgaccg gcgtcagcgg atagaggtcg
     7981 agcacgagac ggtgaccgta ttccgcgacc ggcttcagcg agaacacctg cggccgcacc
     8041 tcggtcttga gatcgagcac cacgcgcagg aatccgtccg acgggcgccc gatgcgaatg
     8101 gcttcgatat acggatcggt gcgctgcaaa tgcgacgcga gctcgtcgag ctcgcgcgat
     8161 ggaacgatcc gcgcgagatc gagcacgacg cgattcggat cgcgcagcac gacgagctga
     8221 tgctcgatcg gcgacgacga ttcgaagatg acgcgcgtgt actcctgcgc cggccatacg
     8281 cgcgccgacg cgatgcgcga cgcatcggcc cacgccgatg gcatcagcca ttgcgccgcg
     8341 ggcagcacga gccagcgcaa tgcgcggcgg cgctcgagcg cgacgagcct cagcgcgccg
     8401 ggccggcggc gagcgcgctc aggcattgct cgcccgcgcg cgagaacgcc tgcacgacca
     8461 gcgcgcgccc gtcggcgtgc accggatgcg acagcgataa tgcgaggtcc ggtgagggca
     8521 agcgcgacgc cacgcgctcc ggccactcga cgatgcacac ggcatcgtcg cggaagtagt
     8581 cggacatccc ggcataatcc cattcatccg gattcatgaa gcgataaaaa tcaaagtgat
     8641 aaaagtataa gctcgaaaca ggataatgct caaccaacgc atacgtcgga ctcttgattg
     8701 gccccgcaac gccgcgcgca cgcaacagtc cccgcgccag cgtcgtcttg ccggtgccaa
     8761 gatcgccttg cagggtgacc accatgccgc cgcggaggat cggcgcgatg ttcgcaccgg
     8821 cacgctcggt cgcggcggcg tccggaagat ggagcgtcag tgttgacacc ggatcgcgat
     8881 ttcgccgcac tcgcctcgcg gatcaccgcg tgggggcgcg agctcggctt cgatgccgtc
     8941 ggcgtgtccg acgccgatct cgcggccgag gaagtgcagc tcctgaactg gctcgcgctc
     9001 ggacgccatg gcgagatgga ttatatggcg cgccatgggg cgcggcgcgc gcggcccgcg
     9061 gcgctcgtgc ccggcaccgt tcgggtggtc acggtgcggc tcgattactg gccggggcac
     9121 gcgcgcccgg ccggcgatgt gctcgccgat tcgcatcggg gctatgtcgc gcgctacgcg
     9181 ctcggccgcg actaccacaa ggtgctgcgc aaacgcctcg cgacgctcgc gcggcgcatc
     9241 gaggacgacg tgggcccgtt cggctaccgc gtgttcaccg acagcgcgcc ggtgctcgag
     9301 gtggcgctcg ccgcgaaatc gggcctcggc tggcgcggca agcacacgct gctcttgagc
     9361 cgcgacaccg ggtcctattt cttcctcggc gagatctaca cgaacctgcc gctgccggtc
     9421 acgccaccgg tgacggtgag cgtgtcggcg gcgtaagtga ctgtgacgtc ggtgtcggtc
     9481 ccgagcgcga ccggcgcgct gacgaccggg ctggcggcga aggtgttcac ttccgcacga
     9541 cggcc
//
LOCUS       DASSVX010000129         6301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_171738, whole genome shotgun sequence.
ACCESSION   DASSVX010000129 DASSVX010000000
VERSION     DASSVX010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171738"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="VFO53_05605"
     CDS             complement(<1..1231)
                     /locus_tag="VFO53_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8875786.1"
                     /translation="MLRISGLTLARGARRLLVDANLTVHDRHKVGLVGANGSGKSSLF
                     AAIRGELIPDAGEIDVPAAWTLAHVAQETAATPVPAIDYVQDGDAELRALERALANSA
                     ALDEQTLAEVHHRFEAIGGYAARARAATLLAGLGVDESRQSHPVVRFSGGWRMRINLA
                     QALMCRSDLLLLDEPTNHLDLDAVLWLEDWLRRYEGTLLLITHDRDFLDGVVDTIVHV
                     DNEKLVAYSGNYAQFERERAARLALTQSACAKQQRQVAHLRSYIDRFRAKATKARQAQ
                     SRIKALERMELIAPAHVDSPFGFSFAPVDTGARQLVRLEHATLGYPGARPILTGVDWS
                     MLSGARIGLLGANGAGKSTLLKALAGMLPPLAGERSVAQQLRLGYFAQHQVEQLRADE
                     TPLWHFTRREPQAREQEL"
     gene            complement(1247..2020)
                     /locus_tag="VFO53_05610"
     CDS             complement(1247..2020)
                     /locus_tag="VFO53_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET8875787.1"
                     /translation="MRVITLNVNGLRAADRKGFARWLARIEPWDVVCLQEIKCAACDV
                     PRTLAAPRKSHATFHPAARKGYSGVALYAKHPPRVGTGFGHAEFDAEGRYLQADFSEL
                     SIVSVYLPSGSSGPHRQASKFRFLEAFLPHLESLVRSGREVVLCGDWNIAHQPIDLTN
                     WRSNQKNSGFLPEERAWLSRVFEIGFVDVFRRVDPRPEQYTWWSNRGQAWQKNVGWRI
                     DYQIATPGIAATARGASIYTNRRFSDHAPLIIDYDYALH"
     gene            2296..3030
                     /locus_tag="VFO53_05615"
     CDS             2296..3030
                     /locus_tag="VFO53_05615"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET8875788.1"
                     /translation="MSDRRYPFQRGPRAIRVDARRIAALLLCALVATAAGRAHAALYK
                     WIDEHGIVHYSDKLPIEAVNRANSELSRQAITLHKTEQARPAVQRVAKSGDNDEQKLR
                     EAERERLLAMRRDRALLESYANEAEIDLAKSRAMATIDGQLQSAHAFVAQMSKRRREL
                     DDKKPTFAPRPVPGAIVREIETIDAEVARQNELIAAKQKESAAVAARYDADKQRFREL
                     RAGPGGGVVTSSDGRLAENLELTSAR"
     gene            3150..4952
                     /gene="ettA"
                     /locus_tag="VFO53_05620"
     CDS             3150..4952
                     /gene="ettA"
                     /locus_tag="VFO53_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HET8875789.1"
                     /translation="MAQYVFTMNRVGKLVPPKRVILKDISLSFFPGAKIGVLGLNGSG
                     KSSLLRIMAGEDKEYEGDAVPMPDLRIGFLPQEPKLDPEATVRSVVEEGMGEVLQAKS
                     RLDEVYAAYAEPDADFDALAAEQARLEAIIAAAGAGAEVQMEVAADALRLPSWDAKIG
                     QLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVEWLEQFLAKFPGTVIAVTHDR
                     YFLDNAAEWILELDRGYGIPWKGNYTSWLTQKEERLGRESRTEAARVKAMQKELEWVR
                     TNPKGRQAKSKARIARFEELSSYEHQKRNETQEIFIPVAERLGNEVIEFRHVSKGYGD
                     RLLIDDLSFKVPPGAIVGVIGPNGAGKSTLFRMITGGEKPTKGYGERVLIDDLSFKVP
                     PGAIVGVIGPNGAGKTTLFRLITGREKPDSGEVVFGHTAQLAVVDQTRESLPNDKTAW
                     EAISGGLDILTVGKFEMPSRAYLGRFNFKGGDQQKNVGQLSGGERGRLHLAATLLKGG
                     NVLLLDEPSNDLDVETLRALEDALSEFAGSALVISHDRWFLDRIATHILAAEDDSKWV
                     FFEGNYHEYEADKKKRLGEEGARPHRPRYKPLMH"
     gene            5032..5205
                     /locus_tag="VFO53_05625"
     CDS             5032..5205
                     /locus_tag="VFO53_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HET8875790.1"
                     /translation="MIKWAIIFFVISVVAGFFGFTGVASGTRTIAKVLFFIALVIFLI
                     VLIFGVLLGTLVF"
     gene            complement(5207..5665)
                     /locus_tag="VFO53_05630"
     CDS             complement(5207..5665)
                     /locus_tag="VFO53_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET8875791.1"
                     /translation="MRLAGPLILLTTLCLGAHIADAQSPTTGTALHATSAGARARAVA
                     RKAVQIGKVSWYGPRFAGRTTASGERFDPRALTMAHRSLPLGAVVDVTNNANGRHVRV
                     RVNDRGPYHGNRVADLSRAASERLGFVDSGVTDATIRVVSLPSDADARSS"
     gene            complement(5755..>6301)
                     /gene="gatB"
                     /locus_tag="VFO53_05635"
     CDS             complement(5755..>6301)
                     /gene="gatB"
                     /locus_tag="VFO53_05635"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018084.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HET8875792.1"
                     /translation="MRARLVAQHGITDYDAAQLTASRDLVDYYNAVCAALPAADAASH
                     KLVANFVLGEFAAARNRDDIAVAAAPVAPGALAHLLRRIADRTLSAKMGRDVFDAMWA
                     GEGDADAIIEQRGLRQISDAAAIETLVEQVIAANAAIVAEFRAGREKAFNSLVGQVMK
                     ASKGKANPAQVNAILRQKLSS"
BASE COUNT         1083 a         2076 c         2126 g         1016 t
ORIGIN      
        1 gaagctcctg ctcgcgtgct tgtggctcgc gcctggtgaa atgccacagc ggcgtctcgt
       61 ccgcgcgcaa ttgctcgacc tggtgctgcg cgaaatagcc gagccttagc tgctgcgcga
      121 ccgaacgctc gccggcgagc ggcggcagca tgccggccag ggccttcagc agcgtcgact
      181 tgcctgcgcc gttcgcgccg aggagaccga tgcgcgcgcc ggagagcatg ctccagtcga
      241 cgcccgtcag gatcggcctg gcaccgggat agccgagcgt cgcgtgctcg agccgcacga
      301 gttggcgcgc tcccgtgtcg accggcgcaa atgaaaaacc gaacggactg tcgacgtgcg
      361 ccggcgcgat cagctccatg cgctcgagcg ccttgatgcg gctttgtgcc tgccgggctt
      421 tcgtcgcctt cgcgcggaag cgatcgatgt acgagcgcag gtgcgcgacc tgccgctgct
      481 gcttcgcgca ggccgattgc gtgagcgcca gcctggccgc gcgctcgcgc tcgaactgtg
      541 cgtagttccc gctgtacgcc acgagctttt cgttgtcgac gtgcacgatc gtgtcgacga
      601 cgccgtcgag gaaatcgcga tcgtgcgtga tcagcagcag cgttccctcg tagcgccgca
      661 gccagtcctc gagccaaagc accgcgtcga gatcgaggtg attcgtcggc tcgtcgagca
      721 gcagcaggtc ggagcggcac atgagcgcct gcgcgagatt gatgcgcatg cgccagccac
      781 cggaaaagcg cacgaccggg tgcgactgcc gtgattcgtc gacgccgagc ccggcgagca
      841 gcgtcgcggc gcgtgcgcgc gcggcatagc cgccgatcgc ctcgaagcgg tggtggactt
      901 ccgcgagcgt ttgctcgtcg agtgccgcac tgttcgccag cgcccgctcg agcgcccgca
      961 actcggcgtc gccgtcctgc acgtagtcga ttgccggaac cggggtggcc gctgtttcct
     1021 gcgcgacgtg cgcgagcgtc cacgcggcgg gcacatcgat ctcgccggcg tccggaatca
     1081 gctcgccacg gatggccgcg aagaggctcg acttgcccga gccgttcgca ccgacgaggc
     1141 cgaccttgtg ccggtcgtga accgtcaggt tcgcgtcgac gaggagacgc cgcgcgccgc
     1201 gcgcgagcgt gaggccggaa atcctgagca cgaaaggggc gccgcctcag tgcagcgcgt
     1261 agtcgtagtc gatgatcagc ggtgcgtgat cggagaagcg gcggttcgtg tagatcgatg
     1321 cgccgcgcgc ggtcgcagcg atcccgggcg tggcgatctg gtaatcgatg cgccagccca
     1381 cgttcttctg ccacgcctgt ccgcggttcg accaccacgt gtattgctcg ggacgcggat
     1441 cgacgcggcg gaacacatcg acgaagccga tctcgaagac gcgcgaaagc caggcgcgct
     1501 cctcgggcag gaagccggaa ttcttctggt tgctgcgcca gttcgtgagg tcgatcggct
     1561 gatgggcaat gttccagtcg ccgcacagca cgacctcgcg cccgcttcgc accagcgact
     1621 cgaggtgcgg caggaacgcc tcgaggaagc gaaatttcga ggcctgccga tgcggtccgc
     1681 tcgagcccga cggcagataa acgctgacga tcgacagctc ggaaaaatcg gcctgcaggt
     1741 agcgtccctc ggcatcgaac tcggcgtgcc cgaacccggt tccgacccgc ggcgggtgtt
     1801 tcgcgtagag cgcaacgccg ctgtagccct tcctcgcggc cgggtggaac gtcgcgtgcg
     1861 acttgcgcgg tgcggcgagc gtgcgcggaa cgtcgcacgc cgcgcatttg atctcctgca
     1921 ggcacacgac gtcccacggc tcgatgcgcg ccagccagcg tgcgaacccc ttgcggtcgg
     1981 ccgcacgaag accgttcacg ttcagcgtga tgacgcgcat gcgtggaggc cgaaaatcgg
     2041 cgagtttacc ggagagcatc catcgatcgc gatccccgcc agcgacagtg gaggcacggc
     2101 ggacgtggcc gccggtcgtt ccggtattct ctttggcatg tgaagtgctt gacaaccgcg
     2161 cattccgtga cgaaggcgcg gctcgcgagt gtgcgtttcg cgcttgccgt gcggggtcac
     2221 aatctgcccc gtcgtttcat tgggaagcgg cgcggtgacc gcgcggagaa ccctgcggac
     2281 aaggtgaaga acccgatgag cgacagacgg tatccattcc agcgcggacc gcgcgcgatc
     2341 cgcgtcgacg cacgccgcat tgcggcgctg ctgctttgcg cactcgtcgc cacggccgcg
     2401 gggcgcgccc acgctgcact gtacaagtgg atcgacgagc acggcatcgt gcactacagc
     2461 gacaagcttc cgatcgaagc ggtcaaccgc gccaattcag aactgagtcg ccaggccatc
     2521 acgctgcaca agaccgagca ggcgcgcccc gcggtacagc gcgtggcgaa gagcggtgac
     2581 aacgacgagc agaagctgcg cgaggccgaa cgcgagcgct tgcttgccat gcgccgcgac
     2641 cgcgcactgc tcgaatccta tgcgaacgaa gccgagatcg acctcgcgaa atcgcgcgcg
     2701 atggcgacga tcgacgggca gctgcagtcg gcgcacgcgt tcgtggcgca gatgtcgaag
     2761 cggcgccggg agctcgatga caagaagccg acgttcgcgc cgaggccggt gccgggcgcg
     2821 atcgtgcgcg agatcgagac gatcgatgcc gaagtcgcgc gccagaacga gttgatcgcg
     2881 gcgaagcaga aggaatcggc ggccgtcgct gcccgctacg atgccgacaa gcagcgcttt
     2941 cgcgagcttc gcgcggggcc gggcggtggc gtggtgacgt cgagcgacgg gcgtcttgcc
     3001 gagaacctgg agcttacgag cgcccgctga ctgcgtcaca ccgaagtggg gtcagagacg
     3061 acttttccgg agctacgaaa aagtcgtctc tgaccctttc gcttcgcctg cgataatccc
     3121 ggtttaggca aaccggtttc gcaactccca tggctcaata cgttttcacg atgaatcgtg
     3181 tgggcaagct cgtgccgccc aaacgcgtca tcctgaagga catctcgctg tcgttcttcc
     3241 ccggcgccaa gatcggcgtg ctgggcctga acggttccgg caaatccagc ctgcttcgca
     3301 tcatggccgg cgaggacaag gaatacgagg gcgacgcggt gccgatgccc gacctgcgca
     3361 tcggcttcct gccgcaggag ccgaagctcg atcccgaggc aacggtcagg agcgtcgtcg
     3421 aggaaggcat gggcgaagtg ctgcaggcga agtcgcgcct cgacgaggtc tacgcggcct
     3481 acgccgagcc cgatgccgat ttcgacgcac tggcggccga acaggcccgg ctcgaggcga
     3541 tcatcgccgc cgccggtgcg ggcgccgagg tccagatgga ggttgcggcc gacgcgctgc
     3601 ggctgccgtc gtgggacgcg aagatcggcc agctctcggg cggcgagaag cgccgcgtcg
     3661 cgctttgccg gctcctgctg tcgaagcccg acatgctgct tctcgacgag ccgacgaacc
     3721 acctcgacgc cgaaagcgtc gagtggctcg agcaattcct cgccaagttt cccgggaccg
     3781 tgatcgcggt gacccacgac cgttacttcc tcgacaacgc cgccgaatgg attctcgagc
     3841 tcgatcgcgg ctacggcatc ccctggaagg gcaactacac gtcctggctc acgcagaagg
     3901 aagagcggct cggccgcgag tcgcgcaccg aggcggcgcg cgtcaaggcg atgcagaagg
     3961 aactcgaatg ggtgcgcacg aatcccaagg gccgccaggc gaagagcaag gcgcgcatcg
     4021 cccgcttcga ggaactgtcg tcgtacgagc accagaagcg caacgagacg caggagatct
     4081 tcatcccggt cgccgagcgt ctcggcaacg aggtgatcga attccgccac gtgagcaagg
     4141 gctacggcga tcgtctgctg atcgacgacc tgtcgttcaa ggtgccgccg ggggcgatcg
     4201 tcggcgtgat cggcccgaac ggcgccggca agtcgacgct gttccgcatg atcaccggcg
     4261 gcgaaaaacc caccaagggc tacggcgagc gcgtcctcat cgacgatctg tcgttcaagg
     4321 tgccgcccgg cgcgatcgtc ggcgtgatcg gccccaatgg cgccggcaag acgacgctgt
     4381 tccgactcat caccggccgc gaaaagcccg attcgggcga agtcgtcttc ggtcacacgg
     4441 cacagctcgc cgtcgtcgac cagacgcgcg aatcgctgcc gaacgacaag accgcatggg
     4501 aagccatttc cggcggcctc gacatcctca ccgtcggcaa gttcgagatg ccctcgcggg
     4561 cctatctcgg ccgcttcaac ttcaagggcg gcgatcaaca gaagaatgtc gggcagcttt
     4621 ccggcggcga gcggggacgg ctgcacctgg ccgcgacgct tctgaagggg ggcaacgtgc
     4681 tgctgctcga cgagccctcg aacgacctcg acgtcgagac gctgcgtgcg ctcgaggatg
     4741 cgctatccga gttcgccggc agcgcgctcg tgatctcgca cgaccgctgg ttcctcgacc
     4801 gcatcgccac gcacatcctc gccgccgagg acgactcgaa gtgggtgttc ttcgaaggca
     4861 actatcacga atacgaggcc gacaagaaga agcgcctcgg cgaggaaggc gcgcgcccgc
     4921 atcggccgcg ctacaagccg ctgatgcatt gacggcatgc aggacaacgg aacccggaac
     4981 ctttagcggg gtccgtgaag ggcgggcaac aacaactgca aggaggccgg catgatcaag
     5041 tgggccatca tcttcttcgt gatctcggtc gtcgccggct tcttcggctt caccggcgtt
     5101 gcgtcgggca cgagaacgat cgccaaggtg ctgttcttca tcgccctcgt gatcttcctc
     5161 atcgtgctga tcttcggggt gctgctcggc acgctggttt tctaggctag ctggatctcg
     5221 cgtccgcgtc gctcggcagc gatacgacgc ggatcgtcgc gtcggtcacg cccgagtcga
     5281 caaatccgag acgctcggac gccgcgcgcg acagatcggc aacccggttg ccgtgatacg
     5341 gcccgcgatc gttgacgcgc acacgcacgt gcctgccgtt ggcgttgttc gtgacgtcga
     5401 ccactgcgcc aagcggcaac gagcgatgcg ccatcgtgag ggcgcgcgga tcgaatcgct
     5461 cgccgcttgc ggtcgttcgc cccgcgaacc gcggcccgta ccacgacacc ttgccgattt
     5521 gcaccgcttt gcgcgccacg gcgcgcgctc tagcgccggc cgacgtcgca tgcaatgcgg
     5581 tgccggttgt cggcgattgc gcatcggcga tgtgggcgcc gaggcaaagc gttgtcagaa
     5641 gaatcagcgg tcctgcgagg cgcatccgtc tcggtcggga agggggtccc tttatgaaac
     5701 gcccggaatc gcaaggttcc ccggtgcgga tggcgcgaga gcgcgccgcc ggcgtcagga
     5761 ggacagtttt tgccgcagga tcgcgttcac ctgcgcgggg ttcgccttgc ctttcgacgc
     5821 cttcatcacc tggccgacga gcgagttgaa cgccttttcc ctgcccgcgc gaaactcggc
     5881 gacgatcgcg gcgttggccg caatcacctg ctcgaccaac gtttcgatgg ccgcggcatc
     5941 cgaaatctgg cgcagcccgc gttgctcgat gatggcgtcg gcgtcgcctt caccggccca
     6001 catcgcatcg aacacgtcgc gccccatctt cgccgacaac gtgcggtcgg cgatgcggcg
     6061 cagcaggtgt gcgagtgccc cgggtgcgac cggcgcggcg gcaacggcaa tgtcgtcgcg
     6121 attgcgcgcg gctgcgaatt cgccgagcac gaagttcgcg acgagcttgt gcgaagcggc
     6181 gtcggccgcg ggcagcgcgg cgcacaccgc gttgtagtag tcgacgagat cgcgtgacgc
     6241 cgtcagctgc gccgcgtcgt aatcggtgat gccgtgctgc gcgacgagcc gcgcgcgcat
     6301 c
//
LOCUS       DASSVX010000130        11523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_738315, whole genome shotgun sequence.
ACCESSION   DASSVX010000130 DASSVX010000000
VERSION     DASSVX010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11523
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738315"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..111
                     /locus_tag="VFO53_05640"
     CDS             <1..111
                     /locus_tag="VFO53_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008049604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET8875793.1"
                     /translation="EDVIIPTSVSDEEARKKFPNGFKTHKPYLRTTAHPK"
     gene            308..1690
                     /locus_tag="VFO53_05645"
     CDS             308..1690
                     /locus_tag="VFO53_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET8875794.1"
                     /translation="MSSPIPMHHPLATLDEDAWDDLLNFIEERRVIPIIGPELLQVDT
                     ESGPRQLYEWIAEKLAVKLGVDTSALPQPFTLNDVVCWFLASRGRREEAYTRLRSIFR
                     DANFAPPLALRQLAQITDFDLFVTTTFDPLLEQAVNLERFGGAQSVDVIAYAPNRVAD
                     LPAERERLTRPVIYHLLGRLSASPTYVISDEDTLEFICALQSEHLTPERLFHELEHNH
                     LLFIGSSFTNWLARLFLRMAKRHRLSDPRDVGEVLADSHSIADARLMNFLQQVSVRTR
                     VYSGAGKFVDELHRRWTTRRRPADVIELPAAQRFLPPERDMPENAVFISYAREDLPAV
                     QRLKAGLDAAGIRTWFDLDRLEGGDDYDRKIQRNIARCSYFIPIVSGTTERRLEGYFR
                     REWSYAIDRSRNIAEGALFIMPVCIDDTNAASAHVPDKFKALHIMRLPGGDVTPAFVQ
                     HLQDFLTARH"
     gene            1696..4941
                     /locus_tag="VFO53_05650"
     CDS             1696..4941
                     /locus_tag="VFO53_05650"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875795.1"
                     /translation="MAPLDDAVAQQPSVDADNPWLGLASFTEQTRAFFYGREEEVAEL
                     SRRAQRKLLTLLFGKSGLGKTSILGAGIVPRLRGLGFCPVYVRIDYSRDAPPPAEQIK
                     QAIFRESEASGEWTHVGVAVPGESLWEFLHHRGDVLRDSAGRTLTPLLIFDQFEEIFT
                     LAQGDDFGRRRAAQFVEELADLVENRPPKAVEARIENDESIADAFDFARSDYRVLIAM
                     REDYLAHLETFKSAMPSITQNRMRLAPMTGEQALDAVVKPGGRLVSEEVAEAIVRFIA
                     GGAELRNAEVEPSLLSLICRELNNTRVAQGRAEISADLLAGSHDTILVEFYERALADQ
                     PTGVRHFIEDQLLTESGFRESLAEERVKKAFAAAAAAPDALATLVDRRLLRIEERLDV
                     RRVELTHDVLCSVVRASRDLRREREARDDAERKLAEQQARERATRKALVRARQIAAVC
                     AVLAIGAVASAGFGYVSMKRAQRAEAQAQQTRALAESARGEAEKLVVYLLDDFHLELE
                     PIGRLDIVASLAKRAVDYYAALPAELRTPETDRNRALALVRYGAALRNQSRLDESGKA
                     LAEAVGTLTKLRSQGDVAEATTVGLGLGLTTQARVASSLNNRAEAIDIANRAVAMLQP
                     LMDAATPSTPARRAYGLATTILGFSQSSENQNEAAIITLEKSRDAFAGIDGLKLDDLP
                     AADGYAEASAWQMAALQNLGRFDDVRKVGDGAHKVTRQVLERRPGDMSALRAEGLILG
                     TLGNVEWIELHMRKALAMAREAAGDWQAMVRLDPTNQIAWNNLADQNVQVSAGLWSSG
                     EIDEARKQMQAALAVEHQVKEVAFIGSVLAIASGYLTQLDADAGDRKGAEAMLAANKR
                     FVALAIRDLPVDAFGRAYAPEFLGYYGYPGNGLGYGALAIPRADGDYVTVRALARASA
                     ARLEQIRNPTASQAELRDSALELAYRTIADASYRLKDYAAADSAIQHALALRKTIPTR
                     TLGDRRDAGTQAVLAGMVAARLGRIDEARRLIDPVLDLQRKLHERTDNEDQLQRVEFA
                     QALYASALAGSPHKAAELKEAAALLDALPPQLRALISVRRVRADIAEAQAS"
     gene            4967..6823
                     /locus_tag="VFO53_05655"
     CDS             4967..6823
                     /locus_tag="VFO53_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HET8875796.1"
                     /translation="MRPEARTAAGSQTPADIDDIRAHLDRGAPWEACDAFREAIAERP
                     HDGELLYWGALAHARAGATQIAHALLDRAEAENAPVALASEIASLRGRLLKDAFHRAP
                     ERDGARQLAVRAREQYLKAYAIAHDPHPGINAAALAMLLGDREAARALAREIRAGLAG
                     RSASSSWDVATEAEARLLLGEYDAAARSYAAACMLAGDNAGMIATMRRQLRLLSRVID
                     EAAQMLTLLRVPDVVAFSGHMIDAEARDVPRFPVALVPSVAAALDRHLGSLHQPIVFA
                     SAACGADLLFIEAALAHRAEVNVVLPFERADFVRTSVAAGGDEWVRRFDDALARATRI
                     IMATEENHLGDDMLFEHAAELVEGLSRLRAAQLETSPGMLCVMDPGSEGGVGGTRAAF
                     ERWSGTIGSPEVIDLRVLRGGGDPAASAVSNQARDASAGVAGGRAVERPHRSWKAMMF
                     ADFAGFSRVQDAFAPQFHERFLEIAAAQIADAGVKPLDAKTWGDGLYVVFDSARDGAE
                     FALGFLERTFDVDWTVAGLASTSRIRVALHAGPVFRGFDPVMGREHFFGSSVTRAARI
                     EPVTQPGTVYASEAFAAALAAAGQAGFTLEYIGRLPLAKAYGESTIYRLDRA"
     gene            6837..8102
                     /locus_tag="VFO53_05660"
     CDS             6837..8102
                     /locus_tag="VFO53_05660"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8875797.1"
                     /translation="MKASLPRTFRALAHRNFRLFFTGQGLSLIGTWLQQVAMGWLTYR
                     LTNSAWLLGVVAFCANVGILLFSNVAGVLADRIDRRRGLLVTQSLMLAQAIVLAVLVA
                     FGWIETWHLIVLALWLGTCSAFDTPLRQSMYVHFIADRGDLANAIALNSMLVNTARVV
                     GPALAGLLLALTGEAACFALNALSFVAVIIAIAKMDWPADIRPAVASGWWASWVEGVS
                     YVRTLEPVRALLALVAALAWTIAPYSSLMPVYAKDIYGGGPHTLGYLLSAAGVGALAS
                     TLHLAHRATVRGLGRLIMFAVATSGAALAAFAYIRIYPLALLLLVLVGGGTILAAASA
                     NTILQTIVDDRLRGRIAAFYTLAFLGIAPLGNLAAGAIAGAFGVPFTFALNGVLAVLA
                     ALWYRRKLPMVRTVLRPIYEELGVPPRTR"
     gene            complement(8187..9005)
                     /locus_tag="VFO53_05665"
     CDS             complement(8187..9005)
                     /locus_tag="VFO53_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HET8875798.1"
                     /translation="MKILISVDIEGIAGVVHPDQTRPGNTEFERARRLMTEEANAAIR
                     GAFAGGATAVIVNDSHGGFRNLLANELDPRAELLLGKPRELGMMAGVEQDCDAVFLVG
                     WHSRAGAAGVLAHTISGFAFARVWMNGAETGEAGLYGALAAEFAVPVALYSGDDAFVT
                     ETAPHFPGAVGVVVKRAHGSRVATSLSPSGACTAIETGAAQAAARAGDLAVRPIASPL
                     TMRVDATSVALADLFAMLPIVRRSGATTVEFASPTMRHAIRVLNSLSAMSFMLR"
     gene            complement(9002..10045)
                     /locus_tag="VFO53_05670"
     CDS             complement(9002..10045)
                     /locus_tag="VFO53_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET8875799.1"
                     /translation="MFDAPRIGTLDSGSRNSIADVEGVSVGHATLADGAVQTGVTVVR
                     PHPGDVYREPLPAASVVLNGFGKTMGLVQLDELGLLESPIALTNTFAVGTVATAMIRR
                     AIAANPSIGRTSPTLNPVVAECNDGWLNDLQAFAIRERHYAMAFDAATADFAQGSVGA
                     GRGMSCFELKGGVGSASRATSDGANVVGALVLANFGKLSQLTLRGVPLGCTLTENARA
                     SSVEQGSLIVVLATDAPLEHRQLRRLALRAGAGIARTGSNFGHGSGDIVIAFSTAERL
                     ARGVRAAKARRTTPDEMLDPLFDAAAEATEQAIVNALFAAETVEGYAGHRRRAITERV
                     PDWQVRLGNAEDQ"
     gene            complement(10052..10945)
                     /gene="gsiD"
                     /locus_tag="VFO53_05675"
     CDS             complement(10052..10945)
                     /gene="gsiD"
                     /locus_tag="VFO53_05675"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HET8875800.1"
                     /translation="MATLPASVQTLTDARVRTPWSEFWRKFKRQKVALVAGGFVVLLV
                     VVAIAAPWIVPYDAENYFDYDALNARPSLAHWFGVDALGRDIFSRILMGARISLAAGF
                     LSVAVGAIIGTLLGLLAGYREGWWDRAIMRLCDVLFAFPGILLAIGIVAILGGGMANV
                     IVAVAVFSIPTFARLVRGNTLALKHLTYIEAARSLGASGRTIVFRHIFPGTVSAVVVY
                     FSLRIGTSIITAASLSFLGMGAQPPTPEWGAMLDAARADMLTAPHEAIFPALAIFLTV
                     LAFNLLGDGLRDALDPKIERR"
     gene            complement(10945..>11523)
                     /locus_tag="VFO53_05680"
     CDS             complement(10945..>11523)
                     /locus_tag="VFO53_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8875801.1"
                     /translation="AIGIVSAVWRNRWPDRVGMTLAVSGISFPAFALGMVLMQVFAVE
                     LNWLPTVGNDTWRHYILPSITLGAAVAAIMARFTRSSFVDILGEDFIRTARAKGLDER
                     RVVVKHGLRNALIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDAVDMRDYPVI
                     QALVLLFSLEFILINLIVDVLYGLINPTIRYR"
BASE COUNT         1884 a         3877 c         3957 g         1805 t
ORIGIN      
        1 gaggacgtca tcatcccgac gtcggtgtcc gacgaagagg cgcgcaagaa gtttccgaac
       61 ggcttcaaga cgcacaagcc ttaccttcgc accaccgcac atccgaagta agcgccgcgg
      121 acacgcctgc ggggcgccgc ctggcgcccc gcaggcgcgc cgacgcgcgc gtttgaccct
      181 ggcacgggaa tcgtggacac ttggccgccg ataaatgggg ccaggctcga atttccaggc
      241 ggcctcgcgt ctcgccgccc gatcgaacgg gtaattcgag cctggccctg cttgtcgccc
      301 caagcgcatg agtagcccga tcccgatgca ccacccgctc gccacgctcg acgaagacgc
      361 gtgggacgac ctgctcaatt tcatcgagga gcgccgggtc atcccgatca tcgggcccga
      421 gctcctgcag gtcgacaccg aaagcgggcc gcggcagctc tacgaatgga tcgccgagaa
      481 gctcgcggtc aagctcggcg tcgacacgtc ggcgctgccg cagccgttca cgctgaacga
      541 cgtcgtgtgc tggttcctcg cgtcgcgcgg acggcgcgag gaagcgtaca cgcggctgcg
      601 cagcatcttc cgcgacgcga acttcgcgcc gccgctggca ctgcgccagc tcgcgcagat
      661 caccgatttc gatctgttcg tcacgacgac gttcgatcca ctgctcgagc aggcggtgaa
      721 cctcgagcgc ttcggcggtg cgcaatcggt ggacgtcatc gcctacgcgc ccaatcgcgt
      781 cgcggacctg cccgccgagc gcgaaaggct gacgcgcccg gtgatctacc atctgctcgg
      841 ccggttgtcg gcgtcgccga cctatgtcat ctccgacgag gacacgctgg aattcatctg
      901 cgcgctgcaa agcgagcacc tgacgcccga gcggctcttc cacgagctcg agcacaacca
      961 cctgctgttc atcggcagca gcttcaccaa ctggctcgca cggctgttcc tgcggatggc
     1021 gaagcggcac cgcctgtcgg atccacgcga cgtcggcgaa gtgctcgccg acagccattc
     1081 gatcgccgat gcgcgcctga tgaacttcct gcagcaggtg agcgtgcgca cgcgcgtcta
     1141 cagcggcgcc ggcaagttcg tcgacgagtt gcaccggcgc tggacgacgc ggcgcaggcc
     1201 tgcggacgtg atcgagctgc cggcggcgca gcgtttcctg ccgcccgagc gcgacatgcc
     1261 ggagaacgcc gtcttcatca gctatgcgcg cgaggacctg cccgcggtcc agcggctgaa
     1321 ggcggggctc gacgcggccg gcatccgcac gtggttcgac ctcgaccggc tcgagggcgg
     1381 tgacgattac gaccgcaaga tccagcgcaa catcgcgcgc tgctcgtatt tcatcccgat
     1441 cgtgtccggc accaccgaac ggcgcctcga aggctacttc cggcgcgaat ggagttacgc
     1501 gatcgaccgt tcgcgcaaca ttgccgaagg cgcgctcttc atcatgcccg tgtgcatcga
     1561 cgacacgaac gccgcctcgg cgcacgtgcc cgacaaattc aaggcgctgc acatcatgcg
     1621 attgccgggt ggcgatgtaa ctccggcgtt cgtgcagcac ctgcaggatt tcctgaccgc
     1681 gcgtcattga agtcgatggc gcctctcgat gacgccgtag cgcagcagcc gtcggtcgac
     1741 gccgacaatc catggctcgg cctcgcgtcg ttcaccgagc agacgcgagc gttcttctac
     1801 ggacgcgaag aggaggtcgc cgagctttcg cggcgcgcgc agcgcaagct cctgacgctg
     1861 ctcttcggca agtcggggct tggcaagacg tcgatcctcg gcgctggcat cgtcccccgc
     1921 ttgcgcgggc tcggcttctg tcccgtttac gtgcgcatcg actattcgcg cgacgcgccg
     1981 ccgccggccg agcagatcaa gcaggccatc ttccgcgaaa gcgaggcgtc gggcgagtgg
     2041 acgcacgtcg gcgtcgccgt gcccggcgaa tcgttgtggg aattccttca tcatcgcggc
     2101 gacgtgttgc gcgactcggc cggacgcacg ctcaccccgc ttctgatctt cgaccagttc
     2161 gaggagatat tcacgctcgc gcaaggcgac gatttcggac gccggcgcgc ggcgcaattc
     2221 gtcgaggagt tggccgacct cgtcgagaat cggccaccga aggcggtcga ggcgcggatc
     2281 gagaacgacg aatcgatcgc cgacgcgttc gacttcgcgc gaagcgacta ccgcgtgctg
     2341 atcgcgatgc gcgaggatta cctcgcgcat ctcgaaacgt tcaagagcgc gatgccgtcg
     2401 atcacgcaga accggatgcg gcttgcgccg atgaccggag agcaggcgct cgacgcggtc
     2461 gtcaagcccg gcggccggct cgtgtcggag gaggtggccg aggcgatcgt gcgcttcatc
     2521 gccggcggcg ccgaactgcg caatgcggaa gtcgagccgt cgctcctgtc actgatctgc
     2581 cgcgagctca acaacacgcg cgtggcccag ggccgcgccg agatctcggc cgaccttctc
     2641 gccgggtcgc acgacacgat cctcgtcgag ttctacgagc gagcgctcgc cgatcagccg
     2701 accggcgtgc gccacttcat cgaagaccag ctcctgaccg agtcgggttt tcgcgaaagc
     2761 ctcgccgagg agcgcgtcaa gaaggcgttc gcggccgcgg ctgctgcgcc tgatgcgctc
     2821 gcaacgctcg tcgatcggcg gctgctgcgc atcgaggagc ggctggacgt gcggcgcgtc
     2881 gagctcacgc acgacgtgct gtgcagcgtc gtgcgcgcaa gccgtgacct tcgacgcgag
     2941 cgcgaggcgc gcgacgacgc cgagcgcaag ctcgcggaac agcaggcgcg cgagcgtgcc
     3001 acccgcaagg cgctcgtccg tgcgcgccag atcgcggcgg tgtgcgcggt gctcgcgatc
     3061 ggcgccgtcg cgagcgcggg gttcggctac gtgagcatga agcgggcgca gcgcgccgag
     3121 gcacaggcgc agcagacgcg cgcgcttgcc gaaagcgcga gaggcgaagc ggaaaagctc
     3181 gtcgtgtacc tgctcgacga cttccatctc gagctcgagc cgatcggacg gctcgatatc
     3241 gtcgcgtcgc tcgccaagcg cgccgtcgac tattacgcgg ccttgcccgc cgagttgcgc
     3301 acgcccgaga ccgatcgcaa ccgcgcgctc gcgctcgtac gctacggcgc cgcgctgcgc
     3361 aaccagtcga ggctcgacga aagcggcaag gcgctcgccg aggcggtcgg cacgctgacg
     3421 aagctgcgca gccagggcga tgttgcggag gcgacgacgg tcggtctcgg cctcggcctc
     3481 acgacgcagg cgcgcgtcgc gtcgagcctc aacaatcgtg ccgaggccat cgatatcgcg
     3541 aaccgcgcgg tggcgatgct gcagccgctg atggatgcgg ctacgccgtc gacaccggcg
     3601 cggcgggcgt acggcctcgc gacgacgatc ctcggctttt cgcagtcgag cgagaaccag
     3661 aacgaggccg cgatcatcac gctggagaag tcgcgcgacg cgtttgccgg catcgatgga
     3721 ttgaagctcg acgacctgcc tgcggcggat gggtatgccg aggcctcggc gtggcagatg
     3781 gcggcattgc agaacctcgg ccgtttcgac gacgtgcgca aggtcggcga cggcgcgcac
     3841 aaggtgacgc ggcaggtact cgagcggcgt cccggcgaca tgtcggcgct gcgcgccgaa
     3901 ggcctgatac tgggcacgct cgggaacgtc gaatggatcg agctccatat gcgaaaggcg
     3961 ctcgcgatgg cacgcgaggc cgcgggcgac tggcaagcga tggtgcgtct cgatccgacc
     4021 aatcagatcg cgtggaacaa cctggcggac cagaatgtgc aggtatctgc cggcctctgg
     4081 tcctcgggcg aaatcgacga agcgcgcaag cagatgcagg ctgcgctcgc cgtcgagcat
     4141 caggtgaagg aagtggcgtt catcggcagc gtcctggcga tcgcgtccgg ctacctgacg
     4201 cagctcgacg cggacgccgg cgacaggaag ggcgccgaag cgatgctcgc ggccaacaag
     4261 cggttcgtcg cgctggcgat ccgcgatttg ccggtcgacg cattcggccg cgcgtacgcg
     4321 ccggaattcc tcggctatta cgggtacccc gggaatggcc tcggctatgg ggcgctggca
     4381 attccgaggg ccgacggtga ttacgtaacg gtacgcgcgc tcgcgcgcgc ctcggccgcg
     4441 cggctcgagc aaatccgcaa cccgaccgct tcgcaggccg aattacgtga cagcgccctc
     4501 gagctcgcat accgaacgat cgcggacgcg tcgtatcgtc ttaaggacta tgcggcggcc
     4561 gacagcgcga tccagcacgc actggcactg cgcaagacga ttccgacgcg cacgctcggc
     4621 gatcggcgcg atgccggcac gcaggccgtc ctcgccggca tggtcgcggc gcggctcggc
     4681 cgaatcgacg aggcacgccg gctgatcgac ccggtgctcg acctgcagcg caagcttcac
     4741 gagcgtacgg acaatgaaga tcaattgcag cgcgtcgaat tcgcacaggc gctctatgcg
     4801 tcggcacttg ccggctcgcc gcacaaggcg gcggagctca aggaagctgc ggcgctgctc
     4861 gatgcgttgc cgccgcaact gcgcgccctg atcagcgtgc gccgcgtgcg tgccgacatc
     4921 gcggaggcgc aggcgtcctg agcgctaacg cccgccacga gcgcgaatga ggcccgaagc
     4981 gcggaccgcg gcgggaagtc aaacgcccgc ggacatcgac gacatccgcg cgcatctcga
     5041 ccgcggcgcg ccgtgggagg cgtgcgacgc gtttcgcgaa gccattgccg agcgccccca
     5101 cgacggcgag ctgctttact ggggagcgct cgcgcatgcg cgggccggcg caacgcagat
     5161 cgcgcacgcg cttctcgacc gcgccgaggc cgagaatgcg cccgtcgcgc tcgcttccga
     5221 gattgcgagc ctgcgcgggc ggctgctcaa ggacgcgttc caccgtgccc ccgaacgcga
     5281 tggcgcgcgg caactcgccg tgcgtgcacg cgagcaatac ctgaaggcgt atgcaatcgc
     5341 gcacgatccg catcccggca tcaacgccgc ggcgctcgcg atgctgctcg gcgaccgcga
     5401 agcggcgcgc gcgctggcgc gcgagatccg ggccggcctt gcaggacgtt cggcaagctc
     5461 gtcctgggat gtcgccaccg aggcggaagc gcgattgctg ctcggagaat acgatgccgc
     5521 ggcgcgaagc tatgcggccg cgtgcatgct ggcgggcgac aatgcgggca tgatcgcgac
     5581 gatgcgacgg cagcttcggc tgctttcgcg cgtgatcgac gaggccgcgc aaatgctgac
     5641 ccttttgcgt gtgccggacg tcgttgcgtt ctcgggccac atgatcgacg cggaagcacg
     5701 cgacgttccg cgctttccgg tcgcgctggt tccgtcggtc gccgccgcgc tcgaccgcca
     5761 cctcggcagc ctgcatcagc cgatcgtgtt cgcgtccgcg gcgtgcggcg ccgacctgct
     5821 gttcatcgag gcggcacttg cgcaccgcgc ggaagtgaac gtcgtgctgc cgttcgagcg
     5881 ggccgacttc gtgcgcacga gcgtcgcggc cggtggcgac gaatgggtgc gccgcttcga
     5941 cgatgcgctt gcccgcgcca cgcggatcat catggcgacg gaggaaaatc accttggcga
     6001 cgacatgctg ttcgagcacg cggcggagct cgtcgaaggc ttgtcgcggc tgcgtgcggc
     6061 acaactcgaa acctcccctg ggatgctctg cgtgatggac ccggggtcgg agggcggcgt
     6121 cggcggcacg cgcgcggcgt tcgagcgctg gagcggcacg atcggctcgc ccgaggtcat
     6181 cgatcttcgc gttcttcgcg gcggcggcga ccctgctgcg tccgcggtga gcaaccaggc
     6241 gcgcgatgct tcggcaggtg ttgctggcgg tcgcgccgtc gagcgcccgc atcgatcgtg
     6301 gaaggcgatg atgttcgccg atttcgccgg cttcagccgc gtgcaggacg cgttcgcgcc
     6361 gcagtttcac gagcgttttc tggaaattgc cgcggcgcaa atcgcggacg ccggggtgaa
     6421 gccgctcgat gccaaaacct ggggcgacgg gctctacgtc gtgttcgact ccgcgcggga
     6481 tggcgccgaa ttcgcgctcg gcttcctcga gcgcacgttc gatgtcgact ggacggtcgc
     6541 aggtcttgca tcgacgagtc gcattcgcgt cgcgctgcat gcgggaccgg tctttcgcgg
     6601 tttcgacccg gtgatgggcc gcgagcattt cttcgggtcg agcgtcacgc gggccgcgcg
     6661 catcgagccg gtcacgcaac ccggcaccgt ctacgcgagc gaagcctttg cggcggcgct
     6721 cgcggcggcc gggcaggccg gattcacgct cgaatacatc gggcgcctgc cgctcgcgaa
     6781 ggcgtacggc gagtcgacga tctaccggct cgaccgcgca tgatcgagcg ggcggcgtga
     6841 aagcttcgct gccgcgcact ttccgcgcac tcgcccaccg caacttccgc ctgttcttca
     6901 ccgggcaagg gctgtcgctg atcgggacct ggctccagca ggtggcgatg ggctggctca
     6961 cgtatcggct gacgaactcg gcatggctgc tcggggttgt cgcgttttgc gcgaacgtcg
     7021 gcatcctgct cttcagcaat gtcgcgggcg tgctcgccga tcgcatcgac cggcgtcgcg
     7081 gccttctcgt gacgcagtcg ctgatgctcg cgcaggcgat cgtgctcgcg gtgctggtgg
     7141 cgttcggatg gatcgaaacg tggcacctca tcgtgctcgc gctgtggctt ggcacgtgct
     7201 cggcgttcga cacgccgttg cggcagtcga tgtacgtgca cttcattgcc gaccgcggcg
     7261 accttgccaa tgcgatcgca ctgaattcga tgctcgtcaa cacggcgcgc gtcgtgggcc
     7321 cggcgctcgc ggggctgctg ctggctctca cgggcgaggc cgcgtgcttc gcgctcaacg
     7381 cgctttcgtt cgtcgcggtc atcatcgcca tcgcgaaaat ggactggccg gcggacatcc
     7441 ggccggcggt ggcgtccggc tggtgggcga gctgggtgga aggcgtcagc tatgtgcgca
     7501 cattggaacc ggtacgcgcg ctgctggcgc tcgtcgccgc actcgcatgg acgatcgcgc
     7561 cctattcgtc gctgatgccg gtgtacgcga aggacatcta cggcggtggc ccgcacacac
     7621 tgggctacct gctgtccgcg gccggtgttg gcgcgctcgc gtcgacgctg cacctcgcgc
     7681 accgggcaac ggtgcgcggc ctcggacggc tcatcatgtt cgccgtcgcg acgagcggtg
     7741 cggcgcttgc ggcgttcgcg tatatccgca tctatccgct cgcgctgctt ctcctggtgc
     7801 tggtcggcgg agggacgatt ctcgccgccg cctcggcgaa cacgatcctg cagacgatcg
     7861 tcgacgatcg cctgcgcgga cgcatcgccg cgttctacac gctagccttc ctcggtattg
     7921 cgccgctcgg caacctcgcc gccggcgcaa ttgcgggcgc attcggcgtg ccgttcacgt
     7981 ttgcgctgaa cggcgttctg gccgtgctcg ccgcactctg gtatcgccgc aagcttccga
     8041 tggtgcgtac cgtattgcgg ccgatctacg aggagctcgg cgtaccgccg cgcacgcgct
     8101 gaacgccggc atcgccggtc taccgcagcc ggttgcgcgc gttcgcggct actcggcgtg
     8161 aggctcgcca cgtccgacga cgcgcgtcaa cgcagcatga acgacatcgc cgacaggctg
     8221 ttcagaaccc ggatcgcgtg gcgcatcgtc ggcgaggcaa actccaccgt tgtggcgccg
     8281 ctgcggcgaa cgatcggcag catcgcgaaa agatcggcca gcgcgacgct cgtggcgtcg
     8341 acgcgcatcg tgagcggcga tgcgatcggc cgcaccgcga ggtctccggc gcgcgcggcg
     8401 gcttgcgcgg caccggtttc gatggcggtg cacgcgcccg aaggcgacag gctcgtcgcg
     8461 acccggctgc cgtgcgcgcg cttgacgacg acgccgaccg cgcccggaaa gtgcggcgcc
     8521 gtttccgtca cgaacgcatc gtcgccgcta tagagcgcaa cgggcaccgc gaattccgcg
     8581 gcgagcgcgc cgtacaaacc cgcctcgccg gtctcggcac cattcatcca gacgcgcgca
     8641 aaggcaaagc cgctgatcgt gtgcgcgagg acgccggcgg cgcctgcgcg cgaatgccag
     8701 ccgacgagga atacggcgtc gcagtcctgc tcgacgccgg ccatcatgcc aagctcgcgc
     8761 ggcttgccga gcagcagttc ggcgcgtgga tcgagctcgt tcgcaagcag gttgcgaaag
     8821 ccgccgtgcg aatcgttgac gatgaccgcg gtggcgccgc cggcgaacgc gccgcgaatg
     8881 gccgcgttcg cttcctcggt catcagccgg cgcgcacgct cgaattcggt attgccgggg
     8941 cgcgtctggt cgggatgaac gacgcccgca atgccttcga tgtcgaccga gatcaggatt
     9001 ttcattggtc ttccgcgttt ccgaggcgca cttgccagtc gggaacgcgc tcggtgatgg
     9061 cgcggcgccg gtgcccggcg tagccctcga ccgtttcggc ggcgaagagc gcgttgacga
     9121 tcgcctgctc ggttgcttct gcggccgcat cgaagagcgg gtcgagcatt tcgtcgggcg
     9181 ttgtgcgccg tgctttcgcc gcgcgaacgc cgcgagcgag ccgctcggcg gtcgagaacg
     9241 cgatcacgat atcgccgctg ccgtgtccga agttcgatcc ggtgcgtgcg attccggcgc
     9301 cggcacgcag cgcgagccgg cgcagttggc gatgctcgag cggcgcatcc gtcgccagca
     9361 cgacgatgag cgatccctgc tcgacgctgg aagcgcgtgc gttttcggtc agcgtgcacc
     9421 caagcgggac gccgcgcagg gtcaactgcg acagcttgcc gaagttcgcg agcacgagcg
     9481 cgccgacgac gtttgctccg tccgacgtcg cgcgcgatgc cgatccgacg ccacccttga
     9541 gctcgaagca cgacatgccg cggccggcac cgacgctgcc ctgcgcgaaa tccgccgtcg
     9601 cggcgtcgaa cgccatcgcg taatggcgct cgcggatggc gaaggcctgc aggtcgttga
     9661 gccagccgtc gttgcattcg gcaacgacgg gattcaacgt cggggacgtg cggccgatcg
     9721 acggattggc cgcaatcgca cggcgaatca ttgccgtcgc cacggttcct acggcgaacg
     9781 tattcgtcaa ggcgatcggc gattcgagca agccgagctc gtcgagctga acgaggccca
     9841 tggttttgcc gaagccgttc agcacgaccg acgccgcggg cagcggctcg cggtagacgt
     9901 cgccgggatg tggacgcacc acggtcacgc cggtttgcac ggcgccgtcg gcgagcgtcg
     9961 cgtgacccac actcacgccc tcgacgtcgg cgatcgaatt ccgggaaccg gaatcaagcg
    10021 tgccgatgcg cggagcgtcg aacattggca cttaccggcg ctcgatcttc ggatcgagcg
    10081 catcgcgcag cccgtcgccg agcaggttga acgcaagcac cgtgaggaag atcgcgagcg
    10141 ccggaaagat cgcttcatgc ggcgccgtca gcatgtccgc gcgggccgca tcgagcatcg
    10201 cgccccattc gggcgtgggg ggctgcgcgc ccatgccgag gaacgaaagg ctcgccgcag
    10261 tgatgatcga cgtgccgatg cgcagcgaga aatagacgac cacggccgag accgtgccgg
    10321 gaaagatgtg ccggaacacg atcgtgcgac ccgacgcacc gaggcttcgc gccgcttcga
    10381 tgtaggtgag gtgcttcagc gcaagcgtgt tgccgcgcac gagccgggcg aaggtcggaa
    10441 tgctgaatac ggccacggcg acgatcacgt tcgccatgcc gccgccgagg atggcaacga
    10501 tgccgatcgc gagcaggatg ccgggaaagg cgaacagcac atcgcaaagg cgcatgatcg
    10561 cgcgatccca ccagccttcc cggtagccgg cgagcaggcc gagcagcgtg ccgatgatcg
    10621 cgccgacggc gaccgacagg aatcccgcgg cgagcgaaat gcgcgcgccc atcaggatcc
    10681 ggctgaagat gtcgcgcccc agcgcatcga cgccgaacca gtgcgcaagc gacggtcgtg
    10741 cgttcagcgc gtcgtagtcg aagtagttct cggcgtcgta cggcacgatc cagggtgcgg
    10801 cgatcgcgac gacgacgagc agcacgacga acccgcccgc gacgagcgcc accttctggc
    10861 gcttgaactt gcgccagaac tccgaccacg gcgtgcgcac gcgcgcgtcg gtgagcgtct
    10921 ggacggaggc aggcagcgtc gccatcagcg atagcggatg gtgggattga tcaacccgta
    10981 cagcacgtcg acgatcaggt tgatcaggat gaactcgagc gagaacaaga gcaccagcgc
    11041 ctggatcacc ggatagtcgc gcatgtcgac ggcgtcgacg agaagccggc cgagaccggg
    11101 ccagttgaac accttctcga cgacgatcga gccgccgagc aggaagccga actgcagtcc
    11161 catcatcgtg acgaccggga tcagcgcgtt gcgcaggccg tgcttgacca cgacgcggcg
    11221 ctcgtcgaga cctttcgcgc gtgcagtgcg gatgaagtcc tcgccgagaa tgtcgacgaa
    11281 cgacgagcgc gtgaaccgcg ccatgatcgc ggcaacggcg gcgccgagcg tgatcgacgg
    11341 caggatgtag tggcgccacg tgtcgttgcc gaccgtcggc agccagttga gctcgaccgc
    11401 gaaaacctgc atcagcacca tgccgagcgc aaaggccgga aacgagatgc ccgataccgc
    11461 gagcgtcatg ccgacgcgat cgggccagcg attgcgccat acggccgaaa cgatgccgat
    11521 cgc
//
LOCUS       DASSVX010000131        17128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_604949, whole genome shotgun sequence.
ACCESSION   DASSVX010000131 DASSVX010000000
VERSION     DASSVX010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 17128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17128
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604949"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2348)
                     /gene="purL"
                     /locus_tag="VFO53_05685"
                     /gene_synonym="purI"
     CDS             complement(<1..2348)
                     /gene="purL"
                     /locus_tag="VFO53_05685"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET8875802.1"
                     /translation="MPEVLALRGRSALSPFRVAKLLAAFAALRPGHPVTGLCAHYWHF
                     IEAARALAPGERTTLERLLAYGPADAADGGAHRPGLLVLPRFGTISPWSSKATDIAHN
                     CGLDAITRIERGVAFDVAAQPGAGLDADDRAALLPLIHDRMTQTVVANVDAAQKLFTH
                     VAPRPLATIPLHARGRAALEEADAALGLALSGDEIDYLVDAFTRLRRDPSDAELTMFA
                     QANSEHCRHKIFNATWRIDGAPQPDTLFAMIRATHAAHPQGTLVAYADNSSVIEGGPA
                     ERFHPDREGRYRADRALVHVLMKVETHNHPTAIAPFPGAATGAGGEIRDEGATGTGAK
                     PKAGLVGYTTSHLRIPALPQPWESAPYGKPDRIASALSIMQEGPIGAASFNNEFGRPN
                     LTGYFRTFEMRIGERMRGYHKPIMIAGGIGNIDVAHVHKKRLAGGALLIQLGGPGMLI
                     GMGGGAASSMATGVNTADLDFDSVQRGNAEMERRAQEVIDRCTQLGAANPILSIHDVG
                     AGGLSNALPELVHGAGVGATIDLRAIPSEESGMAPREIWCNEAQERYVLAIAPGDLAR
                     FRAVCERERCPFAVVGHATGSGQLVVDDPRFGNVPVDVALDVILGKPPRMHRDVARRA
                     PALLPLDLAGVSVRDAAYRVLRFPAVADKTFLVAIGDRTVGGLCARDPMVGPWQVPVA
                     DVGVTLRDFSGYEGEAMALGERTPLALIDAPAAGRMAVGEAITNIAASAVRSLSDVKL
                     SANWMAAAGEPGEDAALYVTVRAVALDFCVPLGLSIPVGKDSL"
     gene            complement(2382..3110)
                     /locus_tag="VFO53_05690"
     CDS             complement(2382..3110)
                     /locus_tag="VFO53_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET8875803.1"
                     /translation="MIEIKDVSKWYGTFQVLTDCTTNVEKGEVIVVCGPSGSGKSTLI
                     KAVNALEPIQKGDIFVDGIRVNDPKTNLPKLRARVGMVFQHFELFPHLTVRQNLSLAQ
                     VKVLGRSQSEADARGLKYLDRVGLAAHKSKFPGQLSGGQQQRVAIARALSMDPICMLF
                     DEPTSALDPEMINEVLDVMVQLAKDGMTMMVVTHEMGFANKVANRVIFMDSGRIVEDA
                     SKADFFGKPRSERAQQFLAKILHH"
     gene            complement(3175..3867)
                     /locus_tag="VFO53_05695"
     CDS             complement(3175..3867)
                     /locus_tag="VFO53_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453382.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8875804.1"
                     /translation="MFGHFDYQEILRNLPYLMKVGMTFTLTLTGLVVVGGVIFGTLLA
                     MMRLSSIKPLSVLATTYINLMRSVPLVLVIFWFYFLVPYIGAWITGADQPVKVGAFLS
                     SVITFTMFEAAYYAEIMRAGIQSVARGQMWAGYALGLNYWQTMGKIVLPQAFRNMVPI
                     LLTQTIVMFQDTSLVYVLSITDFLGAASKVAERDGRLVEMYLFVALVYFVMCIAGSYV
                     VRRLQRRIAIIR"
     gene            complement(3877..4632)
                     /locus_tag="VFO53_05700"
     CDS             complement(3877..4632)
                     /locus_tag="VFO53_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET8875805.1"
                     /translation="MHYHWNWTIFWDMTPEGTGTYMHTLLVGLVWTLATGLAAWVIAL
                     ILGSILGVVRTAPNRVAAGIANAYVEFFRNIPLLVQMFLWYFVIPELLPRNLGDAIKQ
                     MAPPWASFVPAVLCLGFFTAARVAEQVRAGIMALPSGQRMAGTALGLTLPQTYRYVLL
                     PVVFRIILPPLTSEFLNIIKNSSVALTIGVVELTAAARAMQEFSFQVFEAFTAATLIY
                     IVVNICVVFAMRWVEHRVAVPGFIGRAVVAAGH"
     gene            complement(4760..5665)
                     /locus_tag="VFO53_05705"
     CDS             complement(4760..5665)
                     /locus_tag="VFO53_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET8875806.1"
                     /translation="MRTSKLSLALALLAFATAPVLAQDSGTLKKIKDSGTITIGYRDA
                     SIPFSYLNDQQQPIGYAMDICGKIVDAVKTKLNMPNLKVALNPVTSSTRIPLMANGTI
                     DLECGSTTNNAERKKQVDFGMTYFVIKYRYVAKKSANMNTIADLKGQTVVSTAGTTDI
                     KALNELNSAKNMGMNILSANDHPAAFLMVETGRAKAFLMDDILLAGLVANSKNPSEFH
                     ISSESLGVEPYSLMLRRDDPEFKKVVDDTMAKLFKSGEIEKIYDKWFMSPIPPKNINL
                     NVPISDSLKKVFANPTDDADPNVYK"
     gene            5820..7325
                     /locus_tag="VFO53_05710"
     CDS             5820..7325
                     /locus_tag="VFO53_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET8875807.1"
                     /translation="MAIRHDLDATPLLPVLSIATLREIERRHAQEPLMERAGAAAADI
                     AAAMLAGRAGRIVALAGPGNNGGDAFVCARRLRERGFDVVVVARTDPAGGDAAKARSA
                     LIAAGVPMIAAPPSERPALIVDGLFGVGLSRAPGAPWSAWIAWANASGAPIVALDVPS
                     GLDAATGVANDPTIVAAATATFIALTPGLLTGDGPDHCGEVSVHALGLDADVPDAGVR
                     LDWRAVQSRLPPVLARRVRKSHKGTFGRACIVGGAEGLVGAALLAGRAAIRLGAGRVV
                     VALAARNPPLVDWLAPELMLREAEAPGTDHDAWVVGPGLGAGERAQALLEKVASVDQP
                     IVIDADALNAIAGKPALVDAVARRRAATLATPHPAEAARLLQCATQDVQADRVAAATR
                     LASMLDAHVVLKGAGSVVARPDGTFDINASGNPALSTAGSGDVLAGILGALLAQRIDA
                     AAAMRIGVCLHGAAADMLVARGIGPLGVTASEVIDAARALMNEATREPSRR"
     gene            complement(7420..8394)
                     /locus_tag="VFO53_05715"
     CDS             complement(7420..8394)
                     /locus_tag="VFO53_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HET8875808.1"
                     /translation="MAQAIRFHATGGPDVLTWESVDVAAPGPGEVRVRNTAIGVNFID
                     TYQRSGLYPVTLPSGLGTEAAGVVEAVGPGVQGLAPGDRVASCTGPLGAYSTERLVPA
                     DKLVKLPDGIDERTAAAMMLKGLTVQYLFRQTFPLKAGDTIVFHAAAGGVGLIACQWA
                     RALGVTMIGTVSSDAKAALAREYGCTHTIVYTRENFVERVREITGGKGVPVVYDSIGK
                     DTFPASLDCLAPRGMFVTFGSSSGPVPPFNLIMLSQKGSLYATRPTLFTYASSHAALD
                     AMASELFEHVKNGRIKPDARQTFALKDAAEAHRSLESRKTTGATLLLP"
     gene            complement(8474..8944)
                     /locus_tag="VFO53_05720"
     CDS             complement(8474..8944)
                     /locus_tag="VFO53_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase P"
                     /protein_id="HET8875809.1"
                     /translation="MIEIAIPGRPALRIASALIDFNGTLAVDGRLIEGVAERLRDLSE
                     RIDVQVVTGDTTGTASQALRALPVGVQLLPADGQRAGKGRAIDAMHASETVVIGNGCN
                     DCAALEKAALSIVVVGTEGCAIDALMRAHVACNSICDALDLLLMPRRLVATLRR"
     gene            complement(8945..9268)
                     /locus_tag="VFO53_05725"
     CDS             complement(8945..9268)
                     /locus_tag="VFO53_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HET8875810.1"
                     /translation="MKGTLLRFYVHENRRHRHLLLYEWLLEQAKQMGIHGGSAFRAIA
                     GYGRHGVLHEERFFELAGDLTVEIDFAVSDDDAQRLIERIRAESISIFYVRVPMEYGT
                     IDGNT"
     gene            complement(9265..9666)
                     /gene="crcB"
                     /locus_tag="VFO53_05730"
     CDS             complement(9265..9666)
                     /gene="crcB"
                     /locus_tag="VFO53_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET8875811.1"
                     /translation="MLAWGSLLAVGGGAAIGAWIRWGLGTALNPLFPTLPLGTLVANL
                     AGGLIMGFAMELITRHNVMPPELRLLVTTGFLGGLTTFSTFSAEIATLLLRREWLWTF
                     VGIAAHLIGSLLLTMLGIVVIRALYAWGGPS"
     gene            complement(9699..10190)
                     /locus_tag="VFO53_05735"
     CDS             complement(9699..10190)
                     /locus_tag="VFO53_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HET8875812.1"
                     /translation="MSLTQIGAPGIRPEIAVRAIPDDERMWVPQAPDVWFRPLLLNTV
                     TGGWCNLLRVRRSGVLSRHRHPMLVVGYVVKGRWKYLEHDWTAEEGSFVFEPPGETHT
                     LTVPADCAEMITFFNIAGAMIYVDAQGRQTGYEDVFTKIGMCRAHYRDVGLGDDYVDA
                     FIR"
     gene            complement(10195..10440)
                     /locus_tag="VFO53_05740"
     CDS             complement(10195..10440)
                     /locus_tag="VFO53_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875813.1"
                     /translation="MALSTAIRHLRRGDWQKAHVLVQNDSSDMGFWAHGIVHLLEGDV
                     GNARYWFRRAGRPFPRERDASAEIDALVAAIKQHSAE"
     gene            complement(10506..11459)
                     /locus_tag="VFO53_05745"
     CDS             complement(10506..11459)
                     /locus_tag="VFO53_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET8875814.1"
                     /translation="MSGAAPRQAWTNHGPLDVVALGEAMIEFNEARAGDSRSWLQGFG
                     GDTSNMAIAAARLGARSGYVTRVGDDAFGRRLMALWAAEGVVTDGVSVDPDASTGVYF
                     VAHGANGHEFSYLRQGSAASRMTPASLPLALLRSTRVLHLSAISQAISNSACDACFAA
                     IDIARAAGARIAYDTNLRLKLWRLPRARAVILETLRRVDWALPGLDDASLLFGELDPA
                     QIVEACHAHGSAVVVLKLGRDGCIVSDRNRNERIEGVRVASVDATGAGDCFDGAFVAR
                     MVAGDDAFAAARYANKAAALATVGYGAVAPLPRAADVEALV"
     gene            complement(11478..12113)
                     /locus_tag="VFO53_05750"
     CDS             complement(11478..12113)
                     /locus_tag="VFO53_05750"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HET8875815.1"
                     /translation="MNLCDYLEPLPLVAVLRGITPEETPAVAEALCAEGFRILEVPLN
                     SPRPFESIRLLAAHCGDRCLVGAGTVLDPADVVRVRDAGGALIVMPHADVAVVREAKR
                     LAMVCLPGVATATEAFAALAAGADGLKLFPAEAMVPAVLRAWHAVLPKSTLVFAVGGI
                     RADNMGPWWAEGAVGFGTGSNLYKPGAAPDDVRAAAAAYAGAFRALPKRDR"
     gene            complement(12120..13031)
                     /locus_tag="VFO53_05755"
     CDS             complement(12120..13031)
                     /locus_tag="VFO53_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HET8875816.1"
                     /translation="MAATALVAIDWGTSMARAWCLDASGAIVARRDVALGVKHVRDGN
                     FEAALAKLLDPLPIGLAPRIACGMIGSRQGWVEAPYVDCPASLTALSDHLVEVPLGSL
                     SIVPGVATRDTAGLPDVMRGEETQLLGAVAANESGVLAVLPGTHSKWARVEHGRIVDF
                     TTFMTGELYAVLIDHSMLGRIAGHEPGRLSREAFSQGVARGLDEGELSHDLFGARTLA
                     LRGELASDDVADWLSGLLIGREIRGARAWALRAGIDPSIVRVIGSDALVERYDLALGD
                     AGALARHASHDAAALGLWRIAQRAKLV"
     gene            complement(13039..13485)
                     /gene="moaE"
                     /locus_tag="VFO53_05760"
     CDS             complement(13039..13485)
                     /gene="moaE"
                     /locus_tag="VFO53_05760"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HET8875817.1"
                     /translation="MAVRVQSEDFDAGAAIAGLRSADRGAGAIAAFIGTVRDINDEAT
                     ITGLTLEHYPGMTEAALEDIVDEAKARFDVRDVLVVHRVGALLPGDQIVLVVVSSAHR
                     RPAFDACAFVMDLLKTRAPFWKKERLPDAERWVEARASDDEAAARW"
     gene            complement(13490..13753)
                     /locus_tag="VFO53_05765"
     CDS             complement(13490..13753)
                     /locus_tag="VFO53_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET8875818.1"
                     /translation="MVRLRLVYLARLREAFSSSGELLDLGAHDAPTLASVVATLRARG
                     GAFATELAPGRAVRFAVNHRLARSDHPVVDGDEVAIFPPVTGG"
     gene            complement(13858..14535)
                     /locus_tag="VFO53_05770"
     CDS             complement(13858..14535)
                     /locus_tag="VFO53_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET8875819.1"
                     /translation="MLSKQLVVAFFVGAVAFAPAAFADVPAAAGERNGGFYGGVSLRH
                     AGTGADGLDLGHVASTWGRFASPVTDDNARRALVFGGYRFANDLAVEGSFSSAESYAL
                     RPFDAGMHRGIGLSLTASPELATHSWNADVYTSWSFRKSLSLYGRLGYAQSDSLPAYT
                     LAALPAGDPRRLRDGVNYGVGVRYDITPALGLRLEYARFPRFAGESVTGALPDSDQVQ
                     FGMQLRF"
     gene            complement(14630..15142)
                     /gene="mobB"
                     /locus_tag="VFO53_05775"
     CDS             complement(14630..15142)
                     /gene="mobB"
                     /locus_tag="VFO53_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HET8875820.1"
                     /translation="MRVFGFAGWSGSGKTTLIETLVPRLTGRGLRVSLVKHAHHDFDV
                     DQPGKDSHRHRMAGCDEVLVTSGVRWALMHELRGAPELALADALARLSPCDLVLVEGW
                     KRAAIPKLEVWRERLGKPLLHPGDPRIVGIATDAPDELASSLPAFALRDADAIATFVV
                     DNAVHWAVAN"
     gene            complement(15154..16302)
                     /locus_tag="VFO53_05780"
     CDS             complement(15154..16302)
                     /locus_tag="VFO53_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET8875821.1"
                     /translation="MRIGVPRETKDGERRVALVPDGARALVAAGHDVVVEAGTGAGSG
                     FGDDAYRAAGAAVTSDAGEIWRCPLIVKVKEIQRAEHRLLVPGTTILGFAQLNRDPAL
                     LDAVLRSGVRVIACETVRDANAGLPLLAPMSRIAGRLAPLAGAHALATSAGGNGTLIT
                     GVDAVPAARVVVVGAGNAGGEAARIAARLGCSVRVFSRGAARLAALGLALAQSGTPIA
                     TTRLDAPGAEFDDAIANADLVIGAVLEPGTLSPKLVRRDVVRAMRAGSAIVDVGIDQG
                     GIAATSRMTTLSHPTYVEEGVVHYAVANMPAQVARTATLALAGAALPYVQSLASRGIA
                     AALGADDGLASGVMVWDGVIAHPGLARDAGLPHGERPWCATRARAHAA"
     gene            16423..17031
                     /locus_tag="VFO53_05785"
     CDS             16423..17031
                     /locus_tag="VFO53_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875822.1"
                     /translation="MKHPLVPRSPRPEDAGHGPIIATLERVLADIPAPANGRAELPDS
                     AAKAIARRAAKRAALLSGSLALPPGPLGFVTLLPDLYLIWHTQRQMVADIFGVYGRSA
                     ELTRTHMLYCLFRHAASQVVRDVAVRSAERLVVRQLSGNALRSALGTIGVSVSKRVAG
                     NATGRWIPLAGAAAVGAYAYWDTMQVAGTATRLLGETATAEA"
BASE COUNT         2897 a         5918 c         5677 g         2636 t
ORIGIN      
        1 agcgaatcct tgcccacggg aatcgacagt cccagcggga cgcagaagtc gagcgcaact
       61 gcgcgcaccg tgacgtagag cgcagcatcc tcgcccggct cgccggccgc cgccatccag
      121 ttggcagaca gcttgacgtc ggacagcgag cgcacagcgg acgcggcgat gttcgtgatc
      181 gcctcgccga ccgccatacg gcctgccgcc ggcgcgtcga tcagcgcgag cggcgtacgc
      241 tcgccgagcg ccattgcctc gccttcgtat ccgctgaagt cgcgcagcgt cacgcccacg
      301 tcggccaccg gcacctgcca cggacccacc atgggatcgc gtgcacagag cccgccgacg
      361 gtgcgatcgc cgatggcgac gagaaacgtc ttgtcggcca ccgcgggaaa gcgcagcacc
      421 cgatacgcgg cgtcgcgcac cgacacgccc gcgagatcga gtggcagcag cgccggtgca
      481 cggcgcgcga catcgcgatg cattcgcggc ggcttgccga ggatgacgtc gagcgccacg
      541 tcgaccggca cgttaccgaa gcgcggatcg tcgacgacga gctgcccgga gccggtcgca
      601 tggccgacga cggcgaacgg gcagcgctcg cgctcgcaga ctgcgcgaaa gcgcgcgagg
      661 tcgccgggcg cgatcgcgag aacgtagcgc tcctgcgcct cgttgcacca gatctcgcgc
      721 ggcgccatgc ccgattcctc ggacggaatc gcgcgcaggt cgatggtagc gcccacgccg
      781 gcgccatgga cgagctcggg cagcgcgttg gaaagcccgc ccgcaccgac gtcatgaatc
      841 gacaggatcg gattggctgc tccgagttgc gtgcagcgat cgatcacctc ctgggcgcgt
      901 cgctccatct ccgcgttgcc gcgctgcacg gaatcgaagt cgaggtcggc cgtattgacg
      961 ccggtcgcca tcgaggaagc cgcgccaccg cccatgccga tcagcatgcc cgggccgccg
     1021 agctggatca gcagcgcgcc gccggcaagc cgcttcttgt gcacgtgcgc cacgtcgatg
     1081 ttgccaatgc cgccggcaat catgatcggc ttgtggtagc cgcgcatccg ctcgccgatg
     1141 cgcatttcga acgtgcggaa atagccggtg agattcggac gtccgaactc gttgttgaat
     1201 gacgcggcgc cgatcggacc ctcctgcatg atcgacaacg cggacgcgat gcggtcgggc
     1261 ttgccgtacg gcgccgattc ccagggctgc ggcagcgccg gaatgcgcag atgcgatgtc
     1321 gtgtagccga cgagaccggc cttcggcttg gcgccggtcc ccgtcgcgcc ttcgtcgcga
     1381 atctcgccgc ccgcacccgt cgcggcaccg ggaaacggcg cgatcgcggt cgggtgattg
     1441 tgcgtctcca ctttcatcag cacgtgcacc agcgcgcgat ccgcgcgata gcggccttcg
     1501 cggtcgggat ggaagcgttc ggcggggcca ccttcgatca cggacgaatt gtcggcgtag
     1561 gcgacgagcg ttccctgcgg atgcgccgcg tgcgttgccc ggatcatcgc gaacagcgtg
     1621 tcgggttgcg gcgcgccatc gattcgccac gtcgcattga agatcttgtg ccggcagtgc
     1681 tcggaattcg cctgcgcgaa catcgtgagc tcggcgtcgc ttggatcgcg gcgaagacgc
     1741 gtgaacgcgt cgacgagata gtcgatctcg tcgccggaca gcgcgagccc gagcgctgcg
     1801 tcggcttcct cgagtgcggc gcggcctcgc gcgtgcagcg ggatcgttgc cagcggtcgc
     1861 ggcgcgacgt gcgtgaacag cttttgcgcg gcgtcgacgt tcgcgacgac ggtttgcgtc
     1921 atgcggtcgt ggataagcgg cagcagcgcc gcgcgatcgt cggcgtcgag accggcgccc
     1981 ggttgcgcgg caacgtcgaa cgccacgccc cgctcgatgc gcgttatcgc atcgagaccg
     2041 cagttgtgcg cgatatcggt cgccttggac gaccacggcg agatcgtgcc gaaacgcggg
     2101 agaacgagga gcccggggcg gtgcgcgccg ccgtcagcgg cgtccgccgg accataggcc
     2161 agcaggcgtt cgagcgtcgt ccgctcgccg ggcgccagcg cgcgggcggc ctcgatgaaa
     2221 tgccagtaat gcgcgcagag cccggtgacc ggatgcccgg gacgcagggc ggcgaaggca
     2281 gcaagaagtt tcgcgacgcg gaacggcgac agcgcggagc gaccgcgcag ggcgagaact
     2341 tcgggcacgc gaaattatag cgtccccacc cggcgcgacc cttagtgatg caggatcttg
     2401 gcgaggaact gctgcgcccg ctcggagcgc ggcttgccga agaaatcggc cttcgacgcg
     2461 tcctcgacga tcctcccgga atccatgaag atcacgcggt tcgcaacctt gttcgcgaag
     2521 cccatctcgt gcgtgacgac catcatcgtc atgccgtcct tcgcgagctg caccatcacg
     2581 tcgagcacct cgttgatcat ctccggatcg agcgcggacg tgggctcgtc gaacagcatg
     2641 cagatcgggt ccatcgacaa tgcgcgggca atggccacgc gctgctgctg cccgcccgag
     2701 agctgccccg gaaacttgct cttgtgtgcg gcaaggccga cgcgatcgag atacttgagc
     2761 ccgcgcgcgt ccgcctcgga ctgcgagcgt ccgagcacct tcacctgcgc gagcgacagg
     2821 ttctggcgca cggtgagatg cgggaaaagc tcgaagtgct ggaacaccat gccgacccgc
     2881 gcgcgcagct tcggcagatt ggtcttcggg tcgttgacgc ggatgccgtc gacgaagatg
     2941 tcgcccttct ggatcggctc gagcgcattg acggccttga tcagcgtcga cttgccggag
     3001 cccgagggtc cgcagacgac gatcacctcg cccttctcga cgttggtcgt gcagtcggtg
     3061 agcacctgga aggtgccgta ccacttcgac acgtccttga tttcgatcat cggcatcgcg
     3121 cgttccgatc agcagggaaa ttgacgtgga gccgaggcgc cggaaacacg ggcctcagcg
     3181 aatgatcgcg atcctgcgct gcagccgccg cacgacgtac gacccggcga tgcacatcac
     3241 gaagtacacg agtgcgacga acaggtacat ctcgaccagg cggccgtcgc gctccgccac
     3301 cttggaggcc gcgccgagga aatcggtgat cgacagcacg tagacgagcg acgtgtcctg
     3361 gaacatcacg atcgtctgcg tcagcaggat cggaaccatg ttgcgaaacg cctgcggcag
     3421 cacgatcttg cccatcgtct gccagtagtt gagcccgagc gcatagccgg cccacatctg
     3481 cccgcgcgcc accgactgga tgcccgcgcg catgatctcg gcgtaatacg cggcttcgaa
     3541 catcgtgaac gtgatcaccg acgacaggaa cgcgcccacc ttgacgggct ggtccgcgcc
     3601 ggtgatccag gcaccgatgt acggcacgag gaaatagaac cagaagatca cgagcaccag
     3661 cggcaccgac cgcatcaggt tgatgtacgt ggtggcgagc accgacagcg gcttgatgct
     3721 cgacagccgc atcatcgcga gcagcgtgcc gaaaatgacg ccacccacca cgacgaggcc
     3781 ggtcagcgtc agcgtgaacg tcatccccac cttcatcagg taaggcaggt tgcgcaggat
     3841 ctcctgatag tcgaaatggc cgaacatgcg cgcctttcag tggccggccg ccaccacggc
     3901 acgcccgatg aatcccggca ccgccacgcg gtgctcgacc cagcgcattg cgaacacgac
     3961 gcagatgttg acgacgatgt agatcagcgt cgcagcggtg aacgcctcga acacctggaa
     4021 gctgaattcc tgcatcgcgc gcgcggccgc cgtcaattcg acgacgccga tggtgagcgc
     4081 caccgatgag ttcttgatga tgttcaggaa ctccgacgtg agcggcggca ggatgatgcg
     4141 gaagaccacc ggcaggagca cgtagcgata cgtctgcggc agcgtgaggc cgagcgccgt
     4201 gccggccatc cgctgcccgc tcggcaatgc catgatgccg gcgcgcacct gctcggcgac
     4261 ccgcgcggca gtaaagaatc cgaggcacag cacggccggc acgaacgacg cccacggcgg
     4321 cgccatctgc ttgatcgcat cgccaaggtt gcgcggcaac agctccggaa tcacgaagta
     4381 ccacaggaac atctgcacga gcagcggaat gttgcggaag aactccacgt atgcattggc
     4441 gattcccgca gcgacgcggt tcggcgcggt gcggacgacg ccgaggatcg agccgaggat
     4501 cagcgcaatc acccacgccg caaggcccgt cgcgagcgtc cacaccaacc cgacgagcag
     4561 cgtgtgcatg tacgtgcccg tgccctcggg cgtcatgtcc cagaagatcg tccagttcca
     4621 gtggtaatgc acgctagtgc ctcacgatcg ccatacgtcc gccatcgacg cgcctatggt
     4681 caacgggggg ccgcagcccc ccgtcgttgc aacgacatgc acgcctgcgg ctcgcgccgc
     4741 cgcactccac cgtccagtcc tacttgtaga cgttgggatc cgcgtcgtcg gtcggattgg
     4801 cgaacacctt cttcaacgag tcgctgatcg gcacgttgag gttgatgttc ttcggcggga
     4861 tcgggctcat gaaccacttg tcgtagatct tctcgatctc gccgctcttg aagagcttcg
     4921 ccatcgtgtc gtcgacgacc ttcttgaact cggggtcgtc gcggcgcagc atcaggctgt
     4981 acggctcgac gccgaggctc tccgacgaaa tgtggaattc cgacggattc ttcgagttcg
     5041 caaccaggcc ggcgagcagg atgtcgtcca tcaggaaggc cttcgcgcgc ccggtctcga
     5101 ccatcaggaa cgcggccgga tggtcgttcg ccgacaggat gttcatcccc atgttcttcg
     5161 cgctgttgag ctcgttcagc gccttgatgt cggtggtgcc cgccgtcgac acgaccgtct
     5221 gtcccttgag gtcggcgatc gtgttcatgt tcgccgactt cttcgcgacg tagcgatact
     5281 tgatcacgaa gtaagtcatg ccgaagtcga cctgcttctt gcgttcggca ttgttcgtcg
     5341 tcgagccgca ttcgagatcg atcgtgccgt tcgccatcag cggaatccgc gtcgacgacg
     5401 tcaccggatt cagcgcgacc ttgaggttcg gcatgttgag cttcgtcttc accgcgtcga
     5461 cgatcttgcc gcagatgtcc atcgcgtagc cgatcggctg ctgctggtcg ttgaggtacg
     5521 aaaatggaat cgacgcatcg cgatagccga tcgtgatcgt gccgctgtcc ttgatcttct
     5581 tcagcgttcc agaatcctga gccaggaccg gggcggtggc gaacgcaagc agcgcgagcg
     5641 cgagcgatag cttggaagta cgcatgcaat tcactcctgg aatgaatggg ggcctctggc
     5701 ggcacccggc ggcaggattc tacgggcgca ggcggggaaa gtccaagcct cggcctgaat
     5761 tacacttcca tcgtcccgtt cccgcgcaat ccgcgtcgtc tatcgaacag cgttccgcga
     5821 tggcaatccg acacgatctc gatgcaacgc cgctcctgcc ggtgctctcg atcgccaccc
     5881 tgcgcgagat cgagcggcgg cacgcgcagg agccgctgat ggaacgcgcc ggtgcggcgg
     5941 ctgccgacat cgcggcggcc atgctcgccg gacgcgccgg gcgcatcgtc gcgcttgcag
     6001 ggccgggcaa caacggcggc gacgccttcg tgtgcgcaag gcgcctgcgc gagcggggat
     6061 tcgacgtggt ggttgtcgcg cgcaccgatc cggccggtgg ggatgccgcg aaagcgcgct
     6121 cggcgctcat cgccgccggc gtgccgatga tcgcggcgcc accgtccgag cggccggcgc
     6181 tgatcgtcga cggtcttttc ggcgtcggcc tgtcgcgcgc gcccggcgcg ccgtggtccg
     6241 cgtggatcgc ctgggcgaac gcgagcggcg cgccgatcgt cgctctcgac gtgccgagcg
     6301 gactcgatgc ggcaaccggc gtcgcgaacg atcccaccat cgtcgcggcg gcaaccgcga
     6361 cgttcattgc acttacgccc ggcctcctca ccggcgacgg ccccgatcat tgcggcgagg
     6421 tatcggtgca cgcgctcggg ctcgacgccg acgtgccgga tgcgggcgtg cgtctcgact
     6481 ggcgcgccgt gcagtcgcgc ttgccgccgg tgctggcccg gcgcgtacgc aaatcgcaca
     6541 aggggacgtt cgggcgtgca tgcatcgtcg gcggcgccga aggtctcgtc ggcgcggcgc
     6601 tgcttgccgg acgcgccgcg ataaggctgg gcgcgggccg cgtcgtcgtc gctcttgccg
     6661 cacgcaatcc accgctcgtc gactggctcg cgcccgaact catgctgcgc gaggccgagg
     6721 cccccggcac ggatcacgat gcctgggtgg tcggcccggg gctcggtgcg ggcgagcgcg
     6781 cgcaggcgct gctcgagaag gttgcttccg tcgatcagcc gattgtcatc gacgcggacg
     6841 cattgaacgc gatcgccggc aaacccgcgc tcgtcgatgc ggttgccagg cgccgtgcgg
     6901 cgacgctcgc gacaccgcat ccggccgagg ccgcgcggct gctgcaatgc gcgacgcagg
     6961 atgttcaggc cgaccgcgtc gccgcggcga cgcgccttgc gtcgatgctc gatgcccatg
     7021 tcgtcctgaa aggggccggc agcgttgtcg cgcgtcccga cggcacattc gacatcaacg
     7081 cgagcggcaa tccggctctg tcgacggccg ggtcgggcga cgtgctggca ggcatcctcg
     7141 gcgcgctgct tgcccaacgc atcgatgccg ccgcggcgat gcgcatcggc gtatgcctgc
     7201 acggcgcggc ggccgacatg ctggttgcgc gcggcatcgg tccgctgggg gtcaccgcgt
     7261 ccgaagtgat cgacgcggcg cgggcgttga tgaacgaggc gacgcgcgag ccgtcgcggc
     7321 ggtaatcggg gtcagagacg aatttcgccg gccacactcg tgcatgacgt gaagcagcgc
     7381 cggcgaaatt cgtctctgac cccgaaccgg ttgtcgcgtc taaggcaaca gcagcgtggc
     7441 gccggtcgtc tttcgcgatt cgagcgaacg gtgcgcctcg gctgcatcct tcagcgcgaa
     7501 cgtctgccgt gcatcgggct tgatcctgcc attcttgacg tgctcgaaga gctccgacgc
     7561 catcgcatcg agcgcagcgt gactcgaagc ataggtgaag agcgtcgggc gcgtcgcgta
     7621 cagcgagccc ttctgcgaca gcatgatgag gttgaacggg ggcaccgggc ccgacgagct
     7681 tccgaaggtc acgaacatgc cgcgcggcgc gaggcaatcg agcgacgccg gaaacgtgtc
     7741 cttgccgatc gagtcgtaaa ccacgggaac gcccttgccg ccggtgattt ccctgacccg
     7801 ctcgacgaaa ttctcgcggg tgtagacgat cgtgtgcgtg cagccgtatt cgcgcgcgag
     7861 tgccgccttg gcgtccgagc tcacggtgcc gatcatcgtc acgccgagcg cgcgcgccca
     7921 ctggcacgcg atgaggccga cgccgcccgc cgcagcatgg aagacgatcg tgtcgccggc
     7981 cttcagcgga aacgtctggc ggaacagata ctggaccgtt aggcccttca gcatcatcgc
     8041 ggcagcggtg cgctcgtcga tgccatcggg cagcttgacg agcttgtcgg caggcacgag
     8101 tcgctcggtg ctgtacgcgc ccagcggacc cgtgcagctc gcgacgcgat cgccgggcgc
     8161 aagcccctgc acgcccgggc ccacggcttc gacgacgccg gcggcttcgg tgccgagacc
     8221 cgacggcagc gtcaccggat aaaggccgct gcgctgatag gtgtcgatga agttgactcc
     8281 gatcgcggtg ttgcgcacgc gcacttcgcc gggtcccggc gcggcgacgt cgacgctttc
     8341 ccacgtgagc acgtccggtc cgccggttgc atgaaaacga attgcctgtg ccatttcgtc
     8401 actccatcat ggttgtgatc ggccgcgttc ccgcggcgtc cggttcgtgt gctggctcgc
     8461 caccgcgccg atcctaacgc cggagcgtcg caaccagccg gcgcggcatc aacagcaggt
     8521 cgagcgcgtc gcagatagaa ttgcacgcga cgtgcgcgcg catcagcgca tcgatcgcgc
     8581 agccttcggt tcccacgacg acgatcgaaa gcgccgcctt ctcgagtgcc gcgcaatcat
     8641 tgcacccgtt gccgatcacg accgtttcgc tggcatgcat cgcatcgatc gcacgcccct
     8701 tgcccgctcg ctgtccgtcg gccggcaaca gttgcacgcc taccggaagc gcgcggagcg
     8761 cctgtgacgc ggttcccgtc gtatcgcccg tgacgacctg cacgtcgatt ctttccgaca
     8821 ggtcgcgcaa ccgctcggcc acaccctcga tcagccgtcc gtcgactgcc agtgtaccgt
     8881 tgaaatcaat gagggcgctc gcgatccgca atgccggccg gccgggaatg gcgatttcaa
     8941 tcattcaggt gttgccgtcg atcgtgccgt attccatcgg cacgcgtacg tagaagatgc
     9001 tgatgctctc ggcgcggatg cgctcgatga gccgctgcgc atcgtcgtcg cttaccgcga
     9061 aatcgatctc cacggtgaga tcgcccgcga gctcgaagaa tcgttcctcg tgcagcacgc
     9121 catggcgacc atatcctgcg attgcgcgga acgccgaccc gccgtgaatg cccatttgct
     9181 tcgcctgctc gagcagccac tcgtacagga ggagatgtcg atgccggcga ttctcatgca
     9241 cataaaagcg cagcagcgtg cctttcatga cgggcctccc cacgcgtaga gcgcgcggat
     9301 gacgacgatg ccgagcatcg tcagcagcag ggatccgatg aggtgcgccg cgatgccgac
     9361 gaaggtccat agccattcgc gacgcaagag cagcgttgca atctccgcgg aaaacgtcga
     9421 gaacgtcgtc agcccgccga gaaatccggt cgtcacgagc agcctcagct cgggcggcat
     9481 cacgttgtgg cgcgtgatga gctccattgc aaatcccatg atgagcccac cggcgaggtt
     9541 tgcgaccagg gtgccgagcg gcaacgtcgg gaacagcggg ttgagggccg ttcccagtcc
     9601 ccaccgaatc cacgcgccga tcgccgcgcc gccaccgacg gcaagaagcg agccccacgc
     9661 aagcattcct gtcctcctgc cgcgccgcgg cttcgcttct accggatgaa cgcgtcgacg
     9721 taatcgtcgc ccagtcccac gtcgcgataa tgcgcgcggc acatcccgat cttcgtgaac
     9781 acgtcctcgt agccggtctg gcgcccctgc gcatcgacgt agatcattgc gccggcgatg
     9841 ttgaagaatg tgatcatctc ggcacagtcg gccggcaccg tcagcgtgtg cgtttcgccg
     9901 ggcggctcga acacgaagct gccctcctcc gccgtccagt cgtgctcgag gtatttccag
     9961 cgtcccttga ccacgtagcc gacgacgagc atcgggtggc ggtgccgcga cagcacgccc
    10021 gagcgccgaa cgcgcaacag gttgcaccag ccgccggtga ccgtgttgag cagcagcggc
    10081 cggaaccaca catcgggcgc ctgcggaacc cacatgcgct cgtcatcggg aatggcccga
    10141 accgcgatct cgggacgtat tccgggtgcg ccgatctgcg tgagcgacat cttcctactc
    10201 cgccgaatgc tgcttgatcg ccgccacgag cgcatcgatc tcggcgctcg catcgcgttc
    10261 gcgcgggaat ggacgtcccg cacgccggaa ccagtagcgc gcattgccca cgtcgccttc
    10321 gagcaaatgc acgatgccgt gggcccagaa gcccatgtcg ctgctgtcgt tctgcacgag
    10381 cacgtgcgcc ttttgccaat cgccacgccg cagatgccgg atcgcggtac tgagtgccat
    10441 cgcgtgcctc ctggtgctat gcgatcaggt agcctccgtc gaccgggatg atcgcaccag
    10501 tcacgctaga cgagcgcctc gacatcggca gcgcgcggca gcggcgcgac cgcgccgtag
    10561 ccaaccgtgg cgagcgcagc ggccttgttc gcgtaccgtg ccgcggcgaa cgcgtcgtcg
    10621 ccggcgacca tgcgcgcaac gaatgcgcca tcgaagcaat cgccggcccc ggtggcatcc
    10681 acgctcgcga cgcgaacgcc ttcgatgcgc tcgttgcgat tgcgatcgga aacgatgcag
    10741 ccgtcgcgcc cgagcttcag cacgacgacg gcagagccgt gcgcatgaca cgcctcgacg
    10801 atctgcgcag gatcgagctc gccgaacagc agcgatgcgt cgtcgagtcc aggaagcgcc
    10861 cagtcgacgc ggcgcagcgt ctcgagaatc accgcccgcg cgcgcggcaa ccgccacagc
    10921 ttcaaccgca gattcgtgtc gtaggcgatg cgcgcacccg ctgcgcgggc gatgtcgatc
    10981 gcggcgaagc acgcatcgca cgccgagttc gagatcgcct ggctgatggc cgataggtgc
    11041 aacacgcgcg tcgagcgcag cagcgcgagc ggcagcgatg cgggcgtcat tcgcgacgcc
    11101 gccgagccct ggcgcaggta cgagaactcg tgcccattag caccatgcgc gacgaagtag
    11161 acgccggtgg acgcgtcggg gtcgacgctc acgccatcgg ttaccacacc ttcggcggcc
    11221 catagcgcca tgagccgtcg gccgaatgca tcgtcgccca cgcgcgtgac atagccgctg
    11281 cgcgcgccaa ggcgtgcagc ggcgatcgcc atgttcgacg tgtcgccgcc gaatccctgc
    11341 agccagctgc gcgaatcgcc ggcgcgcgcc tcgttgaact cgatcatcgc ctcgccgagc
    11401 gcgacaacgt cgagcgggcc atgattcgtc cacgcttgac gcggcgctgc cccgctcatc
    11461 gtcattgctc gcgaagctca gcgatcgcgt ttgggcagcg cgcgaaacgc gcccgcatac
    11521 gccgccgccg cggcgcgcac gtcgtccggc gcagcacccg gcttgtacag gttcgagccg
    11581 gtgccgaaac ccacggcacc ctcggcccac cacggaccca tgttgtcggc gcgaatgccg
    11641 ccgaccgcaa atacgagcgt cgacttcggc agcactgcgt gccaggcgcg aagcacggcg
    11701 ggaaccatcg cctcggcagg gaaaagcttg agcccgtcgg cacccgcggc gagtgcggcg
    11761 aacgcctccg tcgccgttgc cacgccgggg aggcacacca tggcgagacg cttcgcttcg
    11821 cgcaccactg ccacgtcggc atgcggcatg acgatcagtg cgccgcccgc atcgcgaacg
    11881 cgaacgacat cggccgggtc gagcacagtg cctgcaccga cgagacagcg atcgccgcag
    11941 tgcgcggcca gcagccggat cgactcgaac gggcgcggcg agttgagcgg cacttcgagg
    12001 atgcgaaagc cctcggcgca aagcgcctcg gcaacggccg gcgtctcctc gggtgtgatc
    12061 ccccgcagca cggcgaccag tggcaggggt tcgaggtagt cgcagagatt catgtcgggt
    12121 tacaccagtt tcgcgcgctg cgcgatccgc cacaggccga gcgccgccgc atcgtgactc
    12181 gcgtgccggg caagcgcgcc cgcgtcgcca agcgcgagat catagcgctc gacgagcgcg
    12241 tcgctgccga tgacccgcac gatcgaagga tcgatccctg cgcggagcgc ccatgcgcgc
    12301 gcgccacgga tctcgcgtcc gatcagaagg cccgacagcc agtcggccac gtcgtcggac
    12361 gcgagctcgc cacgcaacgc cagcgtacgt gcgccgaaca gatcgtgcga gagctcgccc
    12421 tcgtcgagcc cgcgcgccac gccctgagaa aacgcctcgc gtgacaggcg ccccggctcg
    12481 tggcctgcaa tgcgcccgag catgctgtga tcgatgagca ccgcgtagag ctcgccggtc
    12541 atgaacgtcg taaagtcgac gatgcgccca tgctcgacgc gcgcccattt gctgtgcgtg
    12601 cccggaagca ccgcgagcac cccgctttca ttcgcggcga cggcgccgag cagttgcgtc
    12661 tcctcgccgc gcatcacgtc cggaaggccc gcggtatcgc gcgtggcgac accgggaacg
    12721 atcgagagcg aaccgagcgg cacttcgacg agatggtcgg acagcgcggt caacgacgcc
    12781 ggacaatcga cgtacggcgc ttcgacccag ccctggcggc tgccgatcat cccgcacgcg
    12841 atgcgcggtg cgagtccgat cggcaacggg tcgagcaact ttgcgagcgc cgcctcgaag
    12901 ttgccgtcac gcacgtgctt gaccccgagt gcgacgtcgc gccgggccac gatcgcgccg
    12961 ctcgcgtcga ggcaccatgc gcgggccatc gacgtgcccc aatcgatcgc gacgagcgcg
    13021 gtggccgcca acgttgctct accagcgcgc cgcggcttcg tcgtcgcttg cgcgggcctc
    13081 gacccagcgc tcggcgtcgg ggagacgctc cttcttccag aacggcgcgc gcgttttcag
    13141 cagatccatc acgaacgcgc aggcgtcgaa cgccgggcga cggtgcgcgc tcgacacgac
    13201 gacgagcacg atctgatcgc ccggcaggag cgcgccgacg cggtggacga cgagcacgtc
    13261 gcggacgtcg aagcgcgcct tggcctcgtc gacgatgtct tccagcgcgg cttcggtcat
    13321 gcccggataa tgctcgagcg taagcccggt gatcgtcgcc tcgtcgttga tgtcgcgcac
    13381 cgtaccgatg aacgccgcga tcgcgcctgc gccgcggtcg gcggaacgca agccggcgat
    13441 cgccgcgcct gcatcgaagt cttcggattg aacgcgtact gccatcgaat cagccccccg
    13501 tcaccggcgg gaagatcgcc acttcatcgc cgtcgacgac cgggtgatcg ctgcgcgcaa
    13561 gccgatggtt gacggcgaaa cgcaccgcgc ggcccggcgc gagctcggtc gcgaacgcac
    13621 cgccgcgggc gcgcagcgtg gcgacgacgc ttgcgagcgt cggcgcatcg tgggcgccga
    13681 gatcgagcag ttcaccgctc gacgaaaacg cctcccgcag ccgtgcgaga tagacgagac
    13741 gcagccgaac catcgtggaa acggagtcgc gcatgggcat gcatcaagga cagagcgccg
    13801 agcctcggaa agagggccgg catatcgatt gtacgacgta aggtgagcgc ggccgggtca
    13861 gaaccgcaac tgcatgccga attgcacctg gtcggaatcg ggcagcgcac cggtgaccga
    13921 ttcaccggcg aaacgcggaa agcgcgcgta ttcgagccga agtcccagcg ccggcgtgat
    13981 gtcgtaacgc acgccgacgc cgtaattgac cccatcgcgc agccggcgcg gatcgccggc
    14041 cggcagcgcc gcgagcgtat aggccggcag cgagtcgctt tgggcgtatc cgagccgccc
    14101 gtaaagcgag aggctcttgc ggaacgacca gctcgtgtag acatcggcgt tccatgaatg
    14161 cgtcgccagc tcgggcgacg ccgtaagcga caacccgatg ccgcggtgca tgccagcatc
    14221 gaacgggcgc agcgcatagc tctcggcgct cgaaaacgaa ccctcgaccg cgagatcgtt
    14281 cgcaaagcgg tagccgccga acacgagcgc gcggcgggca ttgtcgtcgg tgacgggcga
    14341 tgcgaagcga ccccaggtcg atgcgacatg gccgagatcg agaccgtcgg ccccggtgcc
    14401 cgcgtggcgg agcgagacgc cgccatagaa cccgccgtta cgctcacccg cggctgccgg
    14461 cacgtcggcg aacgcggccg gcgcaaacgc gacggcgccg acgaaaaacg caacaactaa
    14521 ctgtttcgaa agcattttta gccgtacgtt acggagactg cgggcacgga tcgcggcttt
    14581 ggctgcacgc tgacgggcag gatcaacgta cgcccaccgc gggaattcgt caattggcga
    14641 cagcccagtg gacggcattg tcgacgacga atgtggcgat cgcgtcggca tcccgcaagg
    14701 cgaaggccgg gagcgacgag gcaagctcgt cgggcgcgtc ggtggctatg ccgacgatcc
    14761 gggggtcgcc gggatgcaac agcggctttc cgagccgctc gcgccatacc tcgagcttcg
    14821 ggatcgctgc ccgtttccac ccttcgacca ggaccaggtc gcagggcgaa agccgggcaa
    14881 gcgcgtcggc gagtgccagc tcgggggcac cgcgcaactc gtgcatgagc gcccaacgaa
    14941 cgcctgacgt cacgagcact tcgtcgcaac ctgccatccg gtggcggtgc gaatccttgc
    15001 ccggctggtc gacgtcgaag tcatggtgcg cgtgcttgac gagcgaaacg cgcaacccgc
    15061 ggccggtgag cctcggcacg agcgtttcga tcagcgtggt cttgccgctg cccgaccagc
    15121 cggcgaatcc gaacacgcgc atcgcgatcc ctgtcaggcc gcgtgcgcac gggcacgcgt
    15181 cgcgcaccac ggccgctcgc cgtgcggcag gcccgcgtcc cgcgccagcc cgggatgcgc
    15241 gatgacgccg tcccaaacca tcacgccgga cgcgagtccg tcgtcggccc cgagcgccgc
    15301 ggcgatgccg cggctcgcga ggctttgcac ataaggcagc gccgccccgg cgagcgcgag
    15361 cgtcgccgtc cgtgcaacct gcgccggcat gttcgcgacc gcgtagtgca cgacaccctc
    15421 ctcgacgtac gtcggatgcg agagggtggt catgcgcgac gttgcggcaa tgccgccctg
    15481 gtcgatgccg acgtcgacga tcgccgatcc tgcacgcatc gcgcgcacga cgtcgcggcg
    15541 aacgagcttc ggcgacagcg tgcccggctc gagcaccgcg ccgatgacga ggtcggcgtt
    15601 cgcaatcgcg tcgtcgaatt cggcgcccgg cgcatcgagg cgcgtggtgg cgatcggggt
    15661 gccgctttgc gcaagtgcga gcccgagtgc ggcgagccgc gccgcacccc gcgagaacac
    15721 gcgcacgctg caacccaggc gcgcggcgat gcgcgccgcc tcgccgccgg cgttgcccgc
    15781 gccgaccacg acgacccgcg ccgcgggaac cgcgtcgacg ccggtgatca gcgtgccgtt
    15841 gccgcccgcg ctcgtcgcca gcgcgtgtgc gccggcgagt ggcgcgaggc ggccggcgat
    15901 gcgcgacatc ggcgccagca gcggcaagcc ggcgtttgca tcgcgcacgg tctcgcaagc
    15961 aatgacgcgc acgccggaac gaagcaccgc gtcgagcaac gccggatcgc ggttgagctg
    16021 cgcgaagccg aggatcgtcg tgcccggcac cagcaaccgg tgctccgcgc gctggatttc
    16081 cttcaccttg acgatcagcg ggcaccgcca gatttcgccg gcatccgagg tgacggccgc
    16141 gccggccgcc cggtaggcgt cgtccccgaa tccgctccca gcgccggtcc cggcctcgac
    16201 gacgacgtca tggcccgccg caacgagcgc gcgggcaccg tcggggacca gcgccacgcg
    16261 gcgctcgccg tccttggttt cgcgcggtac gccgatgcgc atcgatgtcg ctcgatcagg
    16321 cagtcgctgg gggaatgcca aattgtatcc ggcacccggc cggccttcgc gcgacgacgc
    16381 atcccccgat gtagactgcg ccatcgcccc catatacggc ccatgaagca tccgctcgta
    16441 ccgcgctcgc cccgaccgga agacgcgggc cacggaccga tcatcgcgac gctcgaacgc
    16501 gtgctcgccg atattccggc acccgcgaac ggacgtgccg agctgccgga cagcgccgcc
    16561 aaggcgattg cgcgccgtgc cgccaagcgg gccgcgctgc tgtcgggctc gcttgccctg
    16621 ccgccgggcc cgctcggctt cgtgacgctg ctgcccgatc tctacctcat ctggcacacg
    16681 cagcgccaga tggttgcgga catcttcggc gtgtacggcc gcagcgccga gctcacgcgc
    16741 acgcacatgc tgtactgcct gttccgtcac gccgcgagcc aggtcgtgcg cgacgtcgcc
    16801 gtgcgcagcg ccgagcgcct cgtcgtccgc cagctctcgg gcaatgcatt gcgaagcgcg
    16861 ctcggaacga tcggcgtgtc ggtgtcgaag cgcgtcgccg gcaatgcgac cggccgctgg
    16921 attccgctcg ccggtgccgc cgccgttggc gcctacgcgt actgggacac gatgcaggtc
    16981 gcaggcaccg ccacgcggct tctcggcgaa acggcgaccg ccgaggcgta gccaagcgat
    17041 cgtccatgtg cggccgctac gaactgtcga gccatcccgc ggcgatcgcg ctcgcgttcg
    17101 ggctgccgtt tccgcccgag atacatcc
//
LOCUS       DASSVX010000132         6754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1588192, whole genome shotgun sequence.
ACCESSION   DASSVX010000132 DASSVX010000000
VERSION     DASSVX010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588192"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VFO53_05790"
     CDS             complement(<1..1106)
                     /locus_tag="VFO53_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET8875823.1"
                     /translation="MEQFAKETLPVSLEAEMRHSYLDYAMSVIVGRALPDARDGLKPV
                     HRRVLFAMHESNIVYNRPYVKCARVVGDVMGKYHPHGDMAIYDALVRMAQDFSLRYPL
                     IDGQGNFGSVDGDNPAAMRYTECRLERIANELLADIDKETVDFVPNYDGKEQEPAVLP
                     SRLPNLLINGSSGIAVGMATNIPPHNLTETINGCLALLASPGLTIDELMEIIPAPDFP
                     TAGIIYGLDGVRDGYRTGRGRVIMRARTHFEDLDKGSRQAIIVDEIPYQVNKANLLVR
                     IGELVREKKLDGISDLRDESDKSGMRVVIELKRGEVPEIVQNNLYKLTQLQDSFGMNM
                     VALVDGQPRLLNLKQFLEHFITHRREVVVRRTRF"
     gene            1468..2238
                     /locus_tag="VFO53_05795"
     CDS             1468..2238
                     /locus_tag="VFO53_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET8875824.1"
                     /translation="MTRRLVSGVAAGLLAAAFAGSVAAQGMKADPRGYVAGPAGCDPK
                     NCAYVVDSWNGPTTNIVRSGSYNGALGLAGNLCWRQGYWTPQMAVAQCEPDLVPKPAP
                     PVAAPPPPAPAPAPAPAPAAPPPPQVQRITLASKALFDFDKAVLKPEGKAAIDSEIIS
                     KLANVQRLELVLVTGHTDRIGTQRYNQRLSERRADAVRDYLVSRGVPRDKIETLGMGK
                     TQPVPGVVCNQKALKALIACLAPNRRVEIEVKGEGVAR"
     gene            2315..3637
                     /locus_tag="VFO53_05800"
     CDS             2315..3637
                     /locus_tag="VFO53_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HET8875825.1"
                     /translation="MTSREPRAVDLRIDARFVIPVEPAGTLTDHALVVDAGRIVALVP
                     FATADREYAPRERVVLPDHVLIPGLVNAHCHAAMTLLRGVADDVPLKAWLEEHIWPRE
                     ARFVAPDFVHDGTLLATAEMLRAGITCCNDMYFYPDAAAQAYEEAGMRAMIGMPILDL
                     PTPYAADADGYLQRGFTARDAFKHVPRLAFSLAPHAPYTVSDDTWRSVVVYARQLDLI
                     VQTHLAETRREVDEARATSRESPLARLDRLGATGPSFVAIHAVHVDARDIELLAGQGC
                     HVVHCAGSNMKLASGIAPVAALLAHGVNVALGTDGAASNNRLDMLDEMRLASLAAKVQ
                     TGDAAALPASTLLRMATLAGAAALGLDARIGSLLPGKDADVTAVRLADVETLPLYDPV
                     SQLVNAAGREHVTDVWVAGERLLDERRLPRIDTQALVSRARMWQARLA"
     gene            3655..4413
                     /gene="ubiG"
                     /locus_tag="VFO53_05805"
     CDS             3655..4413
                     /gene="ubiG"
                     /locus_tag="VFO53_05805"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HET8875826.1"
                     /translation="MQPSDAAIATDRPIGPGNADPAELAKFSALAHRWWDPESEFKPL
                     HDINPLRVGWVERIADGLRGKRVVDVGCGGGIFAEALAALGANVTGIDLSEKGIGVAR
                     LHQYESGTNVDYRLVAAETLALEMPAAFDVVTCLELLEHVPEPASTVAACAALARSGG
                     VVVFSTLNRNPKSYVQAILGAEYVLKLLPRGTHDWSRFLRPSELAAFGRRAQLQLAAM
                     TGMSYNPLTRTYRLSRDTSVNYLAAFTRNERNDR"
     gene            4403..5119
                     /locus_tag="VFO53_05810"
     CDS             4403..5119
                     /locus_tag="VFO53_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET8875827.1"
                     /translation="MTADRVPRPAARRLPVTAVLFDLDGTLADSAGDLAFAVNRMRAE
                     RGLSPVGVDALRPHASAGARGLLGAGMGVSPDDADYPVLRDTFLAYYAAALDRTTRLF
                     EGVAELLDALDERALAFGIVTNKATRFTQPVAAALGLDRRAGAIVSGDTTAHPKPHPA
                     PLLHAAALLRVAPEQCVYVGDDLRDVTAGNAAGMATIVADYGYLGEQGCADGWPATGW
                     IDTPLALLDWLPASRIDAAR"
     gene            complement(5160..5993)
                     /locus_tag="VFO53_05815"
     CDS             complement(5160..5993)
                     /locus_tag="VFO53_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875828.1"
                     /translation="MKAARFASALATFVAGCALAFVVAYWSWQIAGPAALHIAAPPPG
                     DPAATIIAADLFNAAGAHAPAGTPAASVLAGDARLVGIVAQPRQRGYALFRFPAGAKL
                     VAQGEQISPGVTLTAIEPFAITIRDGAGERRFVLRESAAAPAAASAVPARSTSPASSA
                     SRTASCAPPVGFAGNVVRINAELLGGLAGEAGPWRTLLAPATGGLVVREEGGFGAMLG
                     LKSGDRIAQANGIALRVPDDVASAVIRPLIANQGVRIVGSRGGTRHELWLANIACAGN
                     G"
     gene            6060..>6754
                     /locus_tag="VFO53_05820"
     CDS             6060..>6754
                     /locus_tag="VFO53_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET8875829.1"
                     /translation="MNPLLHRLQPYPFERLRAMMAGVTPNAAKRAINLAIGEPKHPTP
                     PLIIEALARAAVDGLANYPMTAGSPALREAIARWLCRRHALDVLDADGEVLPVLGSRE
                     ALFAFGQAVIDGSRVGATVVVPNPFYQIYEGAALLAGARIHCVNADASAGFAHRFDRV
                     PQTVWARTQLLYVCSPDNPTGRVMPLDEWRKLFALSDRYGFVIAADECYSEIHFDEAK
                     PPLGALGAARALGR"
BASE COUNT         1062 a         2335 c         2238 g         1119 t
ORIGIN      
        1 tcgaagcgcg tgcggcgcac gaccacttcg cgccggtgcg tgatgaagtg ctcgaggaac
       61 tgcttgaggt tcagcagccg cggctggccg tcgaccagcg cgaccatgtt catgccgaag
      121 ctgtcctgga gctgcgtcag cttgtacaga ttgttctgga cgatctccgg cacttcgccg
      181 cgcttcaact cgatcaccac gcgcatgccg gatttgtcgg attcgtcgcg caggtcggaa
      241 atgccgtcga gcttcttctc gcgcacgagc tcgccgatgc gcacgagcag gttcgccttg
      301 ttcacctggt acgggatctc gtcgacgatg atcgcctggc ggctgccctt gtcgagatcc
      361 tcgaaatggg tgcgggcgcg catgatgacg cggccgcggc ccgtgcgata cccgtcccgc
      421 acgccgtcga gcccgtagat gattcccgcg gtgggaaaat cgggcgccgg gatgatttcc
      481 atcaactcgt cgatggtgag cccggggctc gcaaggagcg cgagacagcc gttgatcgtt
      541 tcggtcaggt tgtgcggcgg aatgttcgtt gccatgccga ccgcgatgcc cgacgagccg
      601 ttgatcagca ggttcggcag ccgcgacggc agcaccgccg gctcctgttc cttgccatcg
      661 tagttcggca cgaaatcgac ggtttccttg tcgatatcgg ccaggagctc gttggcaatg
      721 cgctcgagcc ggcactcggt gtagcgcatc gccgccgggt tgtcgccgtc gaccgagccg
      781 aaattgccct gcccgtcgat cagcgggtag cgcagcgaaa aatcctgcgc catgcgcacc
      841 agggcgtcgt agatcgccat gtcgccgtgc gggtggtatt tgcccatcac gtcgccgaca
      901 acccgcgcgc atttgacgta cgggcggttg tagacgatgt tcgactcgtg catggcgaag
      961 agcacccgcc gatgcacggg cttcaggcca tcgcgcgcgt cgggcagcgc acggccgacg
     1021 atcacgctca tcgcgtagtc gagatacgag tggcgcatct cggcttcgag gcttaccggc
     1081 agggtttcct tagcgaactg ctccatggac gaaggtgatt ccttgcaata ggggtcgaaa
     1141 caattgaaaa aatgacaaaa acgcggggct ttcgatcagt ttgccggctt gcctgaaccc
     1201 gccgcgggcg cgcggggcgt ttgacgtgcc gcggcagccg cttccgaggt ccttgaatac
     1261 acgcaatttt accacggcgt tgcatcgccg cgacagtcgt tcgccgcttg taatcccggc
     1321 gggcattttc attgctgcgg caaaaatgcg cgtgctatat tgaaccggag cattaattgg
     1381 atcggcgcgc cagggtcgtc aacgccttcg cgaaagtcgc ttcgttcgaa taaaacctgt
     1441 tgaaaaccaa gtcaaagggg tagaacaatg actcgacgtc tcgtgtccgg tgtcgccgcc
     1501 ggcttgctcg cggcagcatt tgctggcagc gttgcggcgc aagggatgaa ggccgacccg
     1561 cgtggctatg tggccggtcc cgccggttgc gatcccaaga actgtgcgta cgtggtcgac
     1621 agctggaacg gccccaccac caacatcgta cgcagcggca gctacaacgg cgcgctcggc
     1681 ctcgccggca atctttgctg gcgccagggg tactggacgc cgcagatggc cgtcgcgcaa
     1741 tgcgaacccg atctcgtgcc gaagccggct ccgcccgtgg ccgcgccgcc gccgcccgcc
     1801 ccggcgcccg ctccggcgcc cgccccggcc gcgcccccgc cgccgcaggt ccagcggatc
     1861 acgcttgcat cgaaggcgct gttcgacttc gacaaggcgg tcctcaagcc ggaaggcaag
     1921 gcggcaatcg acagcgagat catttccaag ctcgccaacg tgcagcgcct cgagctggtg
     1981 ctcgtcaccg ggcataccga tcgtatcggc acccagcgct acaaccagcg gctgtcggag
     2041 cgtcgcgccg atgcggtgcg tgattacctg gtcagccgcg gcgtgccgcg cgacaagatc
     2101 gaaacgctcg gcatgggcaa gacgcaaccg gttccgggtg tcgtctgcaa ccagaaggcg
     2161 ctcaaggcat tgattgcctg cctcgcgccg aaccggcgtg tcgaaatcga ggtgaagggc
     2221 gaaggcgtcg cgcgctgatc gcctctcgcc tcccaaaaag ccccgcgaag tttgcggggc
     2281 tttttgtcga cgcagcggct gctaaactgg ctcgatgacg tcacgggagc cgcgcgcggt
     2341 cgacctgcgc atcgatgcgc gcttcgtcat cccggtcgag ccggccggca cgctcaccga
     2401 tcatgcgctc gtcgtcgatg ccggccgcat cgtcgcgctg gtgcccttcg caaccgccga
     2461 tcgtgaatac gcgccccgcg agcgcgtcgt ccttcccgac cacgtactga ttcccgggct
     2521 ggtcaacgcg cactgccacg ccgccatgac gctgcttcgc ggcgtggccg acgatgtccc
     2581 gctgaaggcc tggctcgagg agcacatctg gccgcgcgaa gcgcgcttcg tcgcacccga
     2641 tttcgtccac gacggcacgc tgctcgccac ggcagagatg ctgcgtgccg gcatcacgtg
     2701 ctgcaacgac atgtattttt atccggacgc cgcggcgcag gcgtacgagg aagccggcat
     2761 gcgggcgatg atcggcatgc cgatcctcga ccttccgacg ccgtacgccg ccgacgccga
     2821 cggctacctg cagcgcgggt tcacggcacg cgatgcgttc aagcacgtgc cgcggctcgc
     2881 gttctcgctg gcgccgcacg ctccctatac ggtgagcgac gacacgtggc gctcggtggt
     2941 cgtctatgcg cgccagctcg atctcatcgt ccagacgcat ctcgcggaaa cgcggcggga
     3001 agtcgacgag gcgcgcgcga cttcgcgcga atcgccgctt gcgcggctcg accggctcgg
     3061 cgcgaccggt ccctcgttcg tcgcgatcca tgcggtgcac gtcgatgcgc gcgacatcga
     3121 actgctcgcc gggcaaggct gtcacgtcgt ccactgcgcc ggctcgaaca tgaagctcgc
     3181 gagcgggatc gcgccggtcg cagcactgct cgcccacggc gtgaacgttg ccctcggcac
     3241 cgacggcgcc gcgtcgaaca atcgcctgga catgctcgac gaaatgcgcc tcgccagtct
     3301 tgcggccaaa gtgcaaaccg gcgacgccgc ggcgctcccg gcatcgacgc tgctgcgaat
     3361 ggcgacgctt gcaggggccg cggcgttggg gctcgacgcg cgcatcggat cgctgctccc
     3421 cggcaaggac gccgatgtga cggcggtgcg gctcgccgac gttgaaacgc taccgttgta
     3481 cgatccggtt tcgcagctcg tcaacgccgc cgggcgcgag cacgtcaccg acgtctgggt
     3541 cgcgggggaa cggctgctgg acgagcggcg tctgccgcgc atcgacacac aggcgctggt
     3601 gagccgcgcg cgaatgtggc aggcgcgcct cgcatgaatc cgacgatccc ctcgatgcaa
     3661 ccatccgacg ccgccatcgc gaccgatcgg ccgatcggcc ccggcaacgc cgaccccgcc
     3721 gagctcgcaa aattctcggc actcgcgcac cgctggtggg accccgaaag cgagttcaag
     3781 ccgctgcacg acatcaaccc gctgcgtgtc ggctgggtgg agcggatcgc ggatggattg
     3841 cgtggcaagc gcgtcgtcga cgtcggttgc ggcggcggca tctttgccga ggcgctcgca
     3901 gcgctcggcg ccaatgtgac gggcatcgat ctttccgaga aaggcatcgg cgtcgcacgc
     3961 ctgcatcaat acgaatcggg cacgaatgtc gactaccggc tcgtcgccgc ggagacgctc
     4021 gcgctcgaaa tgccggcggc gttcgacgtc gtcacctgtc tcgagttgct cgagcacgtg
     4081 ccggagcccg catcgacggt ggccgcatgt gcagcgctcg cacggtcggg cggcgtcgtc
     4141 gtcttctcga cgctcaatcg caatccgaaa tcctacgtgc aggccatcct cggcgccgaa
     4201 tacgtgctga agctcctgcc gcgcggcacg cacgactgga gccgcttcct gcggccctcg
     4261 gagctcgccg cattcggccg tcgcgcgcaa ctgcagctcg ctgcgatgac cggcatgagc
     4321 tacaacccgc tgacgcgcac gtatcgcctc agccgcgaca catcggtcaa ttaccttgcg
     4381 gcgttcacgc gaaacgagcg caatgaccgc tgatcgcgtg ccgcggcccg cagcgaggcg
     4441 cttgcccgtc accgccgtgc tgttcgacct cgacggcacg ctggccgatt cggcgggcga
     4501 cctcgctttc gcggtcaacc ggatgcgtgc cgagcgcggc ctgtctccgg tcggcgtcga
     4561 tgcgctgcgt ccgcacgcct cggccggcgc ccgcggactg ctcggggcgg gcatgggcgt
     4621 gtcgcccgac gacgccgatt atccggtgct gcgcgacacg tttctcgcct attacgcggc
     4681 cgcgctcgac cgcacgacgc gcctcttcga aggtgtcgcc gaattgctcg acgcgctcga
     4741 cgagcgggcg ctcgcgttcg gcatcgtgac caacaaggcc acgcgcttca cgcaacccgt
     4801 cgccgcggcg ctcggcctcg atcgtcgcgc cggcgcgatc gtctccggtg acacgacggc
     4861 gcacccgaag ccgcatccgg cgccactgct gcacgcggcg gccctgcttc gcgtcgcgcc
     4921 ggagcaatgc gtctacgtcg gtgacgatct gcgcgacgtg accgcaggca atgcggcggg
     4981 catggcgacg atcgtcgccg attacggcta tctcggcgag cagggatgcg ccgacggttg
     5041 gcccgcaacc ggatggatcg acacgccgct cgcgctgctc gactggctgc ccgcttcgcg
     5101 catcgacgcg gcgcgctgac ctgcggcatc ggcgcgcatg cgcgcagccg gcaagccgct
     5161 catccgttgc ccgcacaggc gatgttcgca agccacagct cgtgcctcgt gccgccgcgg
     5221 gatccgacga tgcgcacgcc ctgattcgcg atcagcggac ggatgaccgc cgacgcgacg
     5281 tcgtcgggaa cgcgaagtgc gattccattc gcctgcgcga tgcggtcgcc ggatttgaga
     5341 ccgagcatcg cgccgaagcc gccctcctcg cgcacgacga gaccgcccgt cgcgggcgcg
     5401 agcagcgtgc gccagggccc ggcttcgcct gcgagcccgc caagcagctc ggcgttgatg
     5461 cgcacgacgt tgcccgcgaa tccaacgggc ggtgcgcagc ttgccgtgcg tgatgccgat
     5521 gacgcaggcg aggtgctgcg ggccggcacc gcggatgccg ccgccggcgc cgccgcgctc
     5581 tcgcgcagca cgaagcggcg ctcgcccgcg ccgtcacgaa tggtgattgc aaacggctcg
     5641 attgcggtga gcgtgacgcc gggcgagatc tgctcgccct gcgccacgag cttcgcgccc
     5701 gccgggaaac ggaacaacgc ataaccacgc tgccgcggct gcgcgacgat gccgacgagc
     5761 cgtgcgtcgc cggcgagcac cgacgcggcc ggcgtgccgg caggcgcatg cgccccggcg
     5821 gcattgaaga gatcggcggc gatgatcgtc gcggccgggt cgccgggcgg tggcgctgcg
     5881 atgtgcaacg ccgcgggccc ggcgatctgc cagctccaat atgcgacgac gaatgcaagc
     5941 gcacagcccg cgacgaaggt ggcgagtgcc gatgcgaatc gcgccgcctt catcgcgaat
     6001 cgccggacgc ggtgccgccg ctataatcgc gctcgcttgc gtccaaacgt tgctcccgca
     6061 tgaatcccct gctgcaccgg ttgcagccct atccgttcga gcggctccgc gcgatgatgg
     6121 ccggcgtgac gccgaatgcg gcgaagcgtg cgatcaatct cgcgatcggc gaaccgaagc
     6181 atccaacgcc gccgttgatc atcgaagcgc tcgctcgcgc ggccgtcgac ggtctcgcga
     6241 attatcccat gaccgcgggc tcgccggcgt tgcgcgaggc gatcgcgcgc tggctttgtc
     6301 gccggcatgc gctcgatgtg ctcgatgccg atggcgaagt gctgccggtg ctcggcagcc
     6361 gcgaggcgct cttcgcattc gggcaggccg tcatcgacgg cagccgggtc ggcgcaaccg
     6421 tcgtcgtgcc caatccgttc tatcagatct acgaaggcgc tgcgctgctg gccggcgcac
     6481 ggatccattg cgtcaatgcc gatgcgagtg ccggctttgc gcatcgcttc gaccgcgttc
     6541 cgcaaaccgt ctgggcgcgc acgcagcttc tctatgtctg ctcgcccgac aatcccacgg
     6601 gccgcgtgat gccgctcgac gagtggcgca agctctttgc gctgagcgac cggtacggat
     6661 tcgtcatcgc ggcggacgag tgttattcgg agatccattt cgacgaagcg aagccgccgc
     6721 tcggcgcgct cggcgccgcg cgggcgctcg gccg
//
LOCUS       DASSVX010000133         5612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_688231, whole genome shotgun sequence.
ACCESSION   DASSVX010000133 DASSVX010000000
VERSION     DASSVX010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688231"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VFO53_05825"
     CDS             <1..973
                     /locus_tag="VFO53_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8875830.1"
                     /translation="ARAQSKSLVVATFPGTWNEAHRNLLAPYFRKRTGASVTQSIQLA
                     TEQIAKLEAAKGGRPPFDVAILDEPQVFDAAKQGLIDEYPAARSPNFKDLFPVYQDKW
                     GPKISLQVIGIGYNPNKIKTPPKSYDDLLNPAYKGRVGLTALNSQLGMAFLAELNRIK
                     GGTETNFDPAFAALRALLPNVGAVAANLGAYATLWQQEQVDIAPYNFNFVQTLKAKGV
                     PVEFVVPDTGLVGWTTSLHLVKNAVEPELAVQYIDSHLAADMQSEMEKPPYDIIPTNS
                     KVPLAGAITQTVAKTPADLAKIRGFDWTKINPQRTALIERFNREIKL"
     gene            1072..1911
                     /locus_tag="VFO53_05830"
     CDS             1072..1911
                     /locus_tag="VFO53_05830"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875831.1"
                     /translation="MSRDEWPLALPLAALFIVCFIAPFFVLLALSLHADAQSSALSVA
                     QYAKFFGDTFNLSILWSTLWLAVKATLVCLLFGYPIAWVCSRAPARWQSVLVFVVVLP
                     ILTSVVVRTFAWIVILGREGVLNHMLRALHVIDQPVPLLFTEAGVIAVLAQVQMPLMV
                     LPLFATLSRIDRNLGDASAALGAGAWRTFFRITLPLSAPGLVAGCILVFSACATAFVT
                     QTLIGGARLVYMPLHIYQQAIGANNWPFAAAISVVFTIAVMIVIYALNRVGRTSKAYG
                     DAR"
     gene            1895..2755
                     /locus_tag="VFO53_05835"
     CDS             1895..2755
                     /locus_tag="VFO53_05835"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875832.1"
                     /translation="MATLAEPSLREVAAVPRMQSATWDVRSQRVVFGTLTAIAIVFLI
                     APTIIVLLTSFTATESLRFPPQGYSLRWYKALVDADQMQAAAWNSLVVAVWTTLTCVV
                     LGTAGALGIARSASRAARVADVLFMSPLILPALAFGFAALVYIHLLGFHPSIPLLIVG
                     HVIVCVPFVLRTTIAALAQVDPALFDASASLGAGRAMTFRRVTLPLIGRGVGAGAFLA
                     FMASFDNVPVSLFLADERTEVLPIHLWQQIDTNLDVRTAAISGVIIVVTIVLMVLAER
                     FAGLSRQLAR"
     gene            2768..3118
                     /locus_tag="VFO53_05840"
     CDS             2768..3118
                     /locus_tag="VFO53_05840"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HET8875833.1"
                     /translation="MPGISIHVVDVTRGLVAAGMRVEVFALTPSRVRLADATVASSGV
                     VDDPALSARFAPGSYEAVLHIAAFYRRAGVALPAVAFLDVATYRFGIDDPAQHYHLPF
                     KLTPWGYSCFRGGA"
     gene            3130..4920
                     /gene="uraD"
                     /locus_tag="VFO53_05845"
     CDS             3130..4920
                     /gene="uraD"
                     /locus_tag="VFO53_05845"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HET8875834.1"
                     /translation="MTPTLNLLNHASAAQFAMLLDGIYEHSPWIVERACAARPFATLA
                     ALKRAMVDVVRDGGADAQLALIRAHPPLAGKAMQTAELTRESTDEQGRAGLTRCTHEE
                     LARIARLNGDYETRFGFPFVLAVRGPRGLGLARAEIFATFERRLGHPQDFEHAEALRN
                     IHRIAEIRLDERVGFVPELGNRVWDWAEALAVHSDAGYAERGELTVTYLTDAHRAVAA
                     QLLAWMRDDCGFDDVSIDGVGNVVGVYHGVDRSAARLLTGSHYDTVRNAGRYDGRLGI
                     LVPMAAVRELARAGRRMPFGIEVVGFAEEEGQRYRATFLGSSALTGRFDPRWLEQTDA
                     DGITMREAMTHAQLPVDDIAKIRRDRERYLAFVEVHIEQGPVLTDADMPLGVVTSING
                     SLRYAGETLGMASHAGTTPMTGRRDAAAATAEALLYVEKRASATPGVVGTVGMLDVPN
                     GSINVVPGRCRFSLDIRAATDTVRDACAADVLAEMNRICDRRGVRLALQETMRASAAP
                     CADAWRAHWERAVARLGLPVHRLPSGAGHDAMKLHEMMPQAMLFVRGQNAGISHNPLE
                     SITNDDADLAVHAFTGLVEQVAMEPGSTRG"
     gene            5023..>5612
                     /locus_tag="VFO53_05850"
     CDS             5023..>5612
                     /locus_tag="VFO53_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET8875835.1"
                     /translation="MAALAISDTTDGRRVLALSGRLDASSIRRVWAQGRDAIASAPGK
                     RVVVDASNVEYCDGAGIALLVDLIAQRKAGDVQVANLAPAFETLLQQFDVLEVERDQD
                     PPPPRRGSIAEVGVIAAGMAQDIREQVVFVGEVTAALGYAMLHPASIRWKDVWRICER
                     VGADALPIVALISFLLGVILAFQSAIPMRRFGAEIFV"
BASE COUNT          849 a         1929 c         1871 g          963 t
ORIGIN      
        1 tgccagggcg cagtcgaagt cgctggtggt tgcgacgttc cccggcacgt ggaacgaggc
       61 gcatcgcaac ctgctcgcgc cgtatttcag gaagcgcacg ggcgcgagcg tcacgcagag
      121 catccagctc gccaccgagc agatcgcgaa gctcgaggcg gccaagggag gacggccgcc
      181 gttcgacgtc gcgatcctcg acgagccgca ggtgttcgac gcggcaaagc agggcctgat
      241 cgacgagtac ccggccgcca gatcgcccaa tttcaaggat ctctttcccg tctaccagga
      301 caagtggggc ccgaagatct cgctgcaggt gatcggcatc ggctacaacc cgaacaagat
      361 caagacgccg ccgaagtcct acgacgacct gttgaatccg gcgtacaagg gccgtgtcgg
      421 cctgacggcg ctcaacagcc agctcggcat ggcgttcctc gccgagctca atcggatcaa
      481 gggcggcacc gagaccaact tcgatcccgc cttcgcggcg ctgcgggcgc tgctgcccaa
      541 cgtcggcgcg gtggcggcca acctcggcgc gtacgcgacg ctatggcagc aggagcaggt
      601 cgacattgcg ccgtacaact tcaacttcgt gcagacgctg aaggcgaagg gcgtgccggt
      661 cgagttcgtc gtgccggaca ccggcctcgt cggctggacg accagcctgc acctcgtgaa
      721 gaacgcggtc gagcccgagc tcgcggtgca gtacatcgat tcgcatctcg ccgccgacat
      781 gcagtcggag atggaaaagc cgccgtacga catcattccg accaattcga aggtgccgct
      841 ggccggcgcg atcacgcaga cggtcgcgaa gacgcccgcc gacctcgcga agattcgcgg
      901 cttcgactgg acgaagatca acccgcagcg cacggcgctg atcgagcggt tcaaccgcga
      961 gatcaagctg taacggccga aatcggggtt cgaaaccggg gtcggagcaa tgagtcctga
     1021 acgatccgcg caacatcccg agccttgacc ggaatcattg ctccgacccc gatgtcgcgc
     1081 gacgagtggc cgctcgcgct gccgctcgcg gcgctgttca tcgtctgctt catcgcgccg
     1141 ttcttcgtgc tgctcgcgct gagcctgcat gccgatgcgc agtcgagcgc gctctcggtc
     1201 gcgcaatatg caaagttctt cggcgacacg ttcaacctga gcatcctgtg gtccacgctg
     1261 tggcttgcgg tcaaggcaac gctcgtctgc ctgctcttcg gctatccgat cgcgtgggtt
     1321 tgctcgcgcg cgccggcgcg ctggcagagc gtgctcgtgt tcgtcgtcgt gctgccgatc
     1381 ctgacgagcg tcgtcgtgcg cacgttcgcg tggatcgtga tcctcggccg tgaaggcgtg
     1441 ctgaaccaca tgctgcgcgc gctgcacgtc atcgaccagc cggtgccgct gctctttacc
     1501 gaggcaggcg tcatcgccgt gctcgcgcag gtgcagatgc cgctgatggt gcttccgctg
     1561 ttcgcgacgc tgtcgcgcat cgaccgcaac ctcggcgacg cctcggcggc gctcggcgcg
     1621 ggcgcgtggc gcacgttctt ccgaatcacg ctgccgctgt cggcgccggg cctcgtcgcc
     1681 ggatgcatcc tcgtcttctc cgcctgcgcg accgcgttcg tcacgcaaac gctgatcggc
     1741 ggcgcgcggc tcgtctacat gccgctgcac atctatcagc aggcgatcgg cgcgaacaac
     1801 tggccgttcg cggcggcgat ctcggtagtg ttcacgatcg cggtgatgat cgtcatctac
     1861 gcgctcaatc gcgtcggccg cacgagcaag gcgtatggcg acgctcgctg aaccatcgtt
     1921 gcgcgaagtc gccgccgtac cgcgcatgca gagcgcgacc tgggacgtgc gctcgcaacg
     1981 cgtcgtcttc ggcacgctga cggccatcgc catcgtgttc ctgatcgcgc cgacgatcat
     2041 cgtgctgctg acctcgttca cggcgaccga atcgttgcgc tttccgccgc agggctattc
     2101 gctgcgctgg tacaaggcgc tggtcgacgc cgaccagatg caggccgcgg cgtggaacag
     2161 cctggtcgtc gccgtgtgga cgacgttgac ctgcgtcgtt ctcggcaccg ccggcgcgct
     2221 cggcatcgca cgtagcgcat cgcgcgcggc acgcgtcgcc gacgtgctgt tcatgtcgcc
     2281 gctgatcctg cctgcgctgg cgttcggatt cgccgcactc gtctatatcc atctgctcgg
     2341 attccacccg tcgattccgc tgctgatcgt cggccacgtc atcgtctgcg tcccgttcgt
     2401 gctgcgcacg acgatcgcgg cactcgcgca ggtcgatccc gcgctgttcg acgcgtcggc
     2461 gagcctcggc gccggccgcg cgatgacctt tcgccgcgtg acgctgccgc tgatcgggcg
     2521 cggcgtcggc gcgggcgcat ttctcgcctt catggcgtcg ttcgacaacg tgccggtgtc
     2581 gctgtttctc gccgacgagc gcaccgaggt gctgccgatc catctgtggc agcagatcga
     2641 cacgaacctc gacgttcgca cggccgccat ttccggcgtc atcatcgtcg tcacgatcgt
     2701 gctgatggtg ctggccgagc gcttcgccgg cctgtcgcgc caactcgccc ggtagctgga
     2761 agacgcggtg ccgggcatct cgattcatgt cgtcgacgtc acgcgcgggc tcgtcgcggc
     2821 cggcatgcgc gtcgaagtgt tcgcgctcac cccgtcgcgc gttcggctcg ccgacgcgac
     2881 ggtggcgtcg agcggcgtcg tcgacgatcc cgcgctgtcc gcgcgcttcg cgcccgggtc
     2941 gtacgaagcg gtgcttcaca tcgccgcgtt ctaccggcgt gccggcgtcg cgttacccgc
     3001 ggtcgcgttc ctcgacgtcg cgacgtaccg cttcggcatc gacgacccgg cacagcacta
     3061 ccacctcccg ttcaagctga cgccgtgggg ctattcgtgc ttccgcggcg gcgcatagcc
     3121 ggagcgaaga tgacacccac gctgaacctg ctgaatcacg cttcggcggc gcagttcgcc
     3181 atgctgctcg acggcatcta cgagcactcg ccgtggatcg tcgagcgcgc ctgtgcagcg
     3241 cgcccgttcg cgacgctcgc cgcgctgaag cgcgcgatgg tcgacgtggt gcgcgatggc
     3301 ggcgccgatg cgcaactcgc gctgatccgc gcgcatcctc cgcttgccgg caaggcgatg
     3361 cagaccgccg agctgacgcg cgaatcgacc gacgagcagg gaagggcggg actgacccgg
     3421 tgcacgcacg aggagcttgc gcgaatcgcc cggctcaacg gcgactacga gacgcgattc
     3481 ggctttccct tcgtcctcgc cgtacgcgga ccgcgcgggc tcggccttgc gcgggccgag
     3541 atcttcgcga ctttcgagcg ccgccttggc catccgcagg atttcgagca cgccgaggcg
     3601 ctgcgcaaca ttcaccgcat cgccgagatc cgtctcgacg aaagggtcgg cttcgtgccc
     3661 gagcttggca accgcgtctg ggattgggca gaagcgcttg ccgtgcacag cgatgcgggg
     3721 tacgccgagc gcggcgagct caccgtgacc tatctgaccg acgcccatcg cgccgttgcc
     3781 gcgcaattgc tcgcctggat gcgcgacgac tgcggtttcg acgacgtgtc gatcgacggc
     3841 gtcggcaacg tcgtcggcgt ctatcacggc gtcgatcgga gcgcggcgcg gcttttgacc
     3901 ggcagccact acgacacggt gcgaaacgcc ggcaggtacg acgggcggct tggcatcctc
     3961 gtgccgatgg ccgcggtgcg cgagcttgca cgtgccggcc ggcgcatgcc gtttggcatc
     4021 gaggtcgtcg gcttcgccga ggaggaaggg cagcgctacc gcgcgacatt tctcggctcg
     4081 agcgcgctca ccggccggtt cgacccgcgc tggctcgagc aaacggatgc cgatggcatc
     4141 acgatgcgcg aagcgatgac gcatgcgcag ctgcccgtgg acgacatcgc gaaaatccgc
     4201 cgcgatcgcg agcgctatct cgcgttcgtc gaagtgcaca tcgagcaggg accggtgctg
     4261 accgacgccg acatgccgct tggtgtcgtc acgtcgatca acggcagcct gcgctacgcc
     4321 ggcgagaccc tcggcatggc gagccatgcg ggcacgacgc cgatgactgg ccgacgcgat
     4381 gcggcggccg ccaccgcgga ggcgctgctt tacgtcgaaa agcgcgcatc ggccaccccc
     4441 ggcgtcgtgg gcaccgtcgg catgctggac gtgccgaacg gctcgatcaa cgtcgtgccg
     4501 ggccgttgtc gcttcagcct cgatatccgc gccgcgaccg acacggtccg cgacgcctgc
     4561 gccgccgatg tgctggccga gatgaaccgg atctgcgatc ggcgcggcgt gcggctcgca
     4621 ttgcaggaga cgatgcgcgc aagtgcggca ccgtgcgccg acgcatggcg cgcgcactgg
     4681 gagcgcgcgg tggcccgatt gggcctgccc gttcaccgct tgccgagcgg tgccggccac
     4741 gacgcgatga agctgcacga gatgatgccg caggcgatgt tgttcgtgcg cggccagaac
     4801 gcgggcatca gtcacaaccc gctggaatcg atcaccaacg acgacgccga ccttgccgtg
     4861 cacgcgttca cgggtctcgt cgaacaggtt gcgatggaac ccggatcgac gcgcggatga
     4921 ccgccgcccg tggcgtggcc gggtgaacgc gcgttcgcag atgcggccgg atgctaaact
     4981 gcccgtcgcc atggagcgcg cgttccccaa cgcaattcgg tgatggcggc actcgctatc
     5041 tccgacacga ccgacggccg gcgcgtgctg gcactttccg ggcggctcga tgcgagctcg
     5101 atacgccgcg tgtgggcgca agggcgcgat gcaatcgcca gcgcacccgg caagcgcgtc
     5161 gtcgtcgatg cgtcgaacgt cgaatactgc gacggcgccg gcatagcgct tctcgtcgac
     5221 ctgatcgccc agcgcaaggc gggcgacgtc caggtcgcca atctcgcccc cgcgttcgaa
     5281 acgctgctcc agcaattcga cgttctcgag gtggagcgcg atcaggaccc gccgccgccg
     5341 cgtcgcggaa gcatcgccga agtgggcgtc attgcggccg gcatggcgca ggacatccgc
     5401 gagcaggtcg tgttcgtcgg cgaggtcacc gcggcgctcg gctacgcgat gctgcacccg
     5461 gcgtcgatcc gctggaagga tgtctggcgc atctgcgagc gggtgggtgc cgacgcgctg
     5521 ccgatcgtcg cgctgatctc gttcctgctc ggcgtgatcc tcgcgttcca gtcggccatt
     5581 ccgatgcgcc gcttcggtgc cgagattttc gt
//
LOCUS       DASSVX010000134         8633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_971716, whole genome shotgun sequence.
ACCESSION   DASSVX010000134 DASSVX010000000
VERSION     DASSVX010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971716"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /gene="ccmI"
                     /locus_tag="VFO53_05855"
     CDS             complement(<1..445)
                     /gene="ccmI"
                     /locus_tag="VFO53_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HET8875836.1"
                     /translation="MPPAPLFWLVALLLAVGTVTALVWPLLRARPNAPPLDEGAATDV
                     YRDQKRQLDNELAAGAITRAERDAQLDEIAARLAAELAAPLRAAAQASPRASYVAALI
                     LVAAIPATALVLYATFGSPGALTSATEANAHPAMSPEQVVAMVDKL"
     gene            647..2950
                     /locus_tag="VFO53_05860"
     CDS             647..2950
                     /locus_tag="VFO53_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HET8875837.1"
                     /translation="MSDQDKNIRDDSLAYHRRWPPGKISLLPTKQLTNQRDLALAYTP
                     GVAAACDEIVRDPHEAFNLTARGNLVGVVTNGTAVLGLGNIGPLAGKPVMEGKAVLFK
                     KFAGLDCFDIELAERDPDKLVDMICALEPTFGGVNLEDIKAPECFYIERKCRERMNIP
                     VFHDDQHGTAIIVGAAVLNGLRVVGKRFEDVKLVCSGAGAAALACLDLLVTLGVRREN
                     IFVADIKGVVYEGRTEEMDDNKARYAQPTPARALRDILPGADVFLGLSAGRVLKPEWL
                     ATMAPRPLILALANPEPEIMPDVAKTARPDAIIATGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATCINEAMKLACVRALADLATIEQSDIVATAYNMHENLRFGPEYLIPRPFDPRLI
                     VMIAPAVARAAMDSGVATRPIDDFDAYRMRLTSFVYHSGLLMKPIFSAAKQAPKRIVY
                     AEGEDERVLRAVQVVVDEGLAKPILVGRPAVLERRIERFGLRLKPVVDFEVINPEYDD
                     RYRDYWMNYYELTQRKGVSQTYAQIEMRRRLTLIGAMMIHRGDADGMICGTFGTHSLH
                     LHYVDQVIGRRPGACAYAAMNAVMLPNRTVFIADTYVNADPTPEQVGEIAILCAEEIR
                     RFGITPKAALLSHSSFGTSNYPSARKMREALAIIRERAPDLEIDGEMHGDAALSEPVR
                     NAAFPNSRLTGDANLLIMPTLDAANISFNLLKAASGSGVTLGPILLGVAKPVNILTPS
                     ATVRRIVNMTALTVVDCAIERQAKLAY"
     gene            complement(2953..3165)
                     /locus_tag="VFO53_05865"
     CDS             complement(2953..3165)
                     /locus_tag="VFO53_05865"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HET8875838.1"
                     /translation="MSALRGVAPNAGSYVSESDYFADGWQRAYWGENHARLRSIKARY
                     DPHGLFVVHHGVGSEDWSADGFARHA"
     gene            3599..4861
                     /locus_tag="VFO53_05870"
     CDS             3599..4861
                     /locus_tag="VFO53_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET8875839.1"
                     /translation="MIKRVQKFFERLGPGLITGAADDDPSGIATYSQAGAQFGLGMLW
                     SVFLTTPLMVGIQVVSARIGRVTGHGIARNIRDHYPRSLLWFIVSILLLANTINIAAD
                     LGAMGEALQLVVGGPQHGHALVFGVLAVLMQIFIPYRYLAPILKWLTLTLFAYVVVLL
                     TVDIPWAKLIAATLIPPLELTSEYLMLLVGVLGTTISPYLFFWQASQEVEEQRATHGD
                     EPLRDSAKGARAHLQRIRIDTWFGMILSNGIAFSIMLTTAVTLNAAGVTDIQTSAQAA
                     LALKPIAGNFAFTLFAAGIIGTGLLAIPVLAGSAAYAVAEAMKWPIGLGLEPLEAKGF
                     YGIITLATLLGAGIDFTSIDPIRALVWSAVLNGVVAVPIMAVMMRMAVRPDIMGPFVI
                     KRALHWLGWLATGVMAIAVVAMLGSLMV"
     gene            5001..5402
                     /locus_tag="VFO53_05875"
     CDS             5001..5402
                     /locus_tag="VFO53_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875840.1"
                     /translation="MKPLVKSALLAAASVALAGCASYDYGYGYADSQPYYGYDSGYSY
                     GYDAGPYSYDYGPYYSGPSYYGAPSVGLGFTYRDRDRDREYHDRNYDEHRNSDTRHDN
                     NYHRNDADPWRAGRDDPWGGTNKPNDRTSQQ"
     gene            5497..6348
                     /locus_tag="VFO53_05880"
     CDS             5497..6348
                     /locus_tag="VFO53_05880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8875841.1"
                     /translation="MPADNSEQIAQWNGALGERWAAMQREIDRIVVPFGDAALRAAAP
                     QPGERVVDVGCGCGDTSIEIARSVGETGAVLGVDVSQPMLEVARSRAALTNRAHLSFQ
                     NADASEAPLPRETDLLFSRFGVMFFSEPSKAFRHLRKSLRTGGRCVFVCWRAPRDNPW
                     AMTPLSAARMAMRVTPAPPDPYAPGPFAFADQERLHAILSEAGFDAIRVQRFDVPLSL
                     GTTAHSAAQFAVQIGPVSRFVREVGVPHLPTILDAVERALAPLAAADGHVSLNGSTWL
                     VSATNPG"
     gene            complement(6443..8491)
                     /locus_tag="VFO53_05885"
     CDS             complement(6443..8491)
                     /locus_tag="VFO53_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875842.1"
                     /translation="MRAWTVDADDIRVADDFDESLLHRTPEIDAFLSSDRDDKFVVIG
                     TKGFGKTLLLKAKRILYQREGRAACLPMGALLDKPIGDKIFSRELLAFFSVSPLPWSK
                     LWLAAISLATLKHLGAVDELKVGAALNRLLGDEHLHSVIDHFVRLLDFTPSELQRCGA
                     DTDGHLVPRLRAVSSPVAIFIDGVDEYFNKHVEGLASHPSVTGELSPNVWHFAQLGLV
                     EVAYQLRRINHHIKVFAAVRKEAYARLAKTTVMFQQYRGSAVDIAYSPQSLREIFINN
                     VRLMKDSMMVRADRLRTEPLDAFLGRSVVVDAYTREEEEVFEYVCRHTLLRPRDLMSI
                     GERLFALRPEERRSEVRFKEVVHQAATEIAQEYLAEIAPFIGDLELEQLFRRLPGNVL
                     ARDHVERLFLEHSIAIGGDEKHVFCALYRVGLLGYVHHDRVRAQWAQRFLRPGEATLE
                     PVGTLPSSTHYLLHPVLTDVIGRVNPAYLQCVDRVNIVGYARPWREAATVANATSVRA
                     LCVLKGDVHGFGGMMHAGAEAPVRQALEEAVKHWTRRAVIAETGAGDSLLIVHEDPVF
                     LAQAARHILDDVYQATGQPRLRMALHYGDVQMQPGVGDQAPTIVGGNAILCASRVEPH
                     VLPGQIWATEEFRRMLVERPSLWRTTALTTPDGGDRFNVKKQGRPEPDLWVRLYRLEF
                     "
BASE COUNT         1445 a         2866 c         2838 g         1484 t
ORIGIN      
        1 cgagcttgtc gaccatcgcg acgacctgct ccggcgacat cgccgggtgc gcattcgcct
       61 ccgtcgcgct cgtcagggca ccggggctgc cgaaggtcgc gtacagcacg agcgcggtcg
      121 cgggaatcgc ggcgacgagg atcagcgccg cgacgtacga tgcgcgcggc gatgcctgcg
      181 ccgcagcgcg cagcggcgcg gcaagctcgg ccgcgagccg cgcagcaatc tcgtcgagtt
      241 gcgcgtcgcg ttcggcacgc gtgatcgcac ctgccgcgag ctcgttgtcg agctgccgct
      301 tctgatcgcg atagacatcg gtcgcagcac cttcgtcgag cggcggcgcg ttcggacgcg
      361 cgcgcagcag cggccacacg agtgcggtca ccgttcccac ggcgagcagc agcgcgacga
      421 gccagaagag gggagcggga ggcatggaag acgttgcgtg cgggccgatc gggacggcgc
      481 cggcgggcga agaaaagggc tcggccgggc ccgcgcatta tagcgatgca cccgttcgcg
      541 gccggggtgc ggcaccagtg cgagatgcgg ccggcggtat gatgactgcc gcgcgttggc
      601 gttgtcggcg caaccatctc ttcgcgactc tcggcggaac gcaatcatgt cggatcagga
      661 caagaacatt cgggacgatt cgcttgccta tcaccggcgc tggccgccgg ggaaaatctc
      721 gctgctgccg acgaagcagc tcaccaacca gcgcgacctg gcactcgcct acacaccggg
      781 cgtcgcggcc gcatgcgacg aaatcgtgcg cgatccgcac gaggcgttca atctcaccgc
      841 gcgcggcaat ctggtcggcg tggtcaccaa cggcaccgcc gtgctcggcc tcggcaacat
      901 cggtccgctg gccggcaagc cggtgatgga aggcaaggcg gtgctgttca agaagttcgc
      961 cggcctcgac tgcttcgaca tcgagctcgc cgagcgcgat cccgacaagc tcgtcgacat
     1021 gatttgcgcc ctggagccga cgttcggtgg cgtcaacctc gaggacatca aggcgcccga
     1081 gtgcttctac atcgagcgca agtgtcgcga gcggatgaac attccggtgt tccacgacga
     1141 ccagcacggc acggccatca tcgtcggcgc cgccgtgctg aacggactgc gcgtcgtcgg
     1201 caagaggttc gaggacgtga agctcgtctg ttcgggcgcc ggtgccgccg cgctggcctg
     1261 cctcgacctg ctcgtcacgc tcggcgtgcg gcgcgagaac atcttcgtgg ccgacatcaa
     1321 gggcgtcgtc tacgaggggc ggaccgagga gatggacgac aacaaggcgc gctacgcgca
     1381 gccgacgccg gcacgcgcgc tgcgcgacat ccttcctggc gccgacgtct tcctcggctt
     1441 gtccgccggg cgcgtgctga agcccgagtg gctcgcgacg atggcgccga ggccgctgat
     1501 cctcgcactt gccaatcccg agccggagat catgcccgac gtggcgaaga ccgcgcggcc
     1561 cgacgcgatc atcgccaccg gccgctccga cttcccgaac caggtcaaca acgtcctctg
     1621 cttcccgttc atctttcgcg gcgcactcga cgtcggcgcg acgtgcatca acgaggcgat
     1681 gaagctcgcg tgcgtgcgcg cgctggccga cctcgcgacg atcgagcaat cggacatcgt
     1741 cgccaccgcc tacaacatgc acgagaacct gcgcttcggt cccgagtacc tgatcccgcg
     1801 gccgttcgat ccgcggctca tcgtgatgat cgcgccggcc gtcgccaggg cggccatgga
     1861 ttccggcgtc gcaacgcgac ccatcgacga tttcgacgcc tatcgcatgc ggctcacgag
     1921 cttcgtctat cactcgggcc tgttgatgaa gccgatcttc tccgcggcga agcaggcgcc
     1981 gaagcgcatc gtgtacgccg agggcgagga cgagcgggtg ctgcgcgccg tgcaggtcgt
     2041 cgtcgacgaa ggactcgcga agccgatcct cgtcggccgc ccggcggtgc tcgagcggcg
     2101 catcgagcgc ttcggcctgc gcctcaagcc ggtggtcgat ttcgaggtga tcaatcccga
     2161 gtacgacgat cgctatcgcg attactggat gaactattac gagctgacgc aacgcaaggg
     2221 cgtgtcgcag acgtacgcgc agatcgagat gcgccgccgc ctcacgctga tcggcgcgat
     2281 gatgatccat cgcggcgacg ccgacggcat gatctgcggc acgttcggca cgcactcgct
     2341 gcatctgcac tacgtcgatc aggtgatcgg ccggcgcccc ggcgcatgcg cctacgcggc
     2401 gatgaacgcg gtgatgctgc cgaaccgcac ggtgttcatc gccgatacct acgtcaacgc
     2461 cgaccccacg ccggagcagg tgggcgagat cgcgatcctg tgcgccgagg agatacgccg
     2521 cttcggcatc acgccgaagg ccgcgctact gtcgcactcg tcgttcggca ccagcaacta
     2581 tccgtcggca cgcaagatgc gcgaagcgct cgccatcatt cgcgagcgtg cgcccgatct
     2641 cgagatcgac ggcgaaatgc acggcgacgc cgcgctgtcc gagccggtgc gcaacgccgc
     2701 atttcccaat tcacggctga ccggggatgc gaacctgctg atcatgccga cgctcgacgc
     2761 cgcgaacatc tcgttcaacc tgctgaaggc cgcatcaggg tcgggggtga cgctcgggcc
     2821 gatcctgctc ggcgtggcga agccggtgaa catcctcacg ccgtcggcca ccgtgcgccg
     2881 gatcgtcaac atgacggcgc tgaccgtcgt cgattgcgcg atcgagcggc aagcgaagct
     2941 cgcgtactga gcttacgcgt gtcttgcgaa gccgtcggcg ctccagtctt cgctccccac
     3001 gccgtggtgc accacgaaca gcccgtgcgg gtcgtaacgc gccttgatcg accgaagccg
     3061 cgcatggttt tcgccccagt acgcgcgctg ccagccgtcc gcgaagtagt cgctctctga
     3121 aacgtacgat cccgcgttcg gggcgacgcc gcgcaacgcg ctcatcgcgg caacatcgac
     3181 gcgccggcgc tccacgcggc cccgcgccac gtccggggtg tccgcggacc gatggatgtc
     3241 cgggcgcgcc ttccgcgccg ctgttcgcaa gcgcgaacgc gcggagcacc tgcggattca
     3301 tcgcggttcg cccggcctcg gcaatcgccg tctcgggcgt cgatccatcc gctggtttgc
     3361 gtcaggccta cccggtcgtg gatgtagtag gggttctgca agtgcgcgaa gaggtcgcgg
     3421 caggcggtgc cggccggttg cgcaagcagt cggcaagcgg cgaatgcacc tcgacgaggc
     3481 ggccgtcgac gcggcgatcg agctcggccc agcgtgccgc gctcggccac gatggatcgc
     3541 cctggccgct gctatactcc ggcgcccgcg agcggcagtc gatcgtgcgg cgtccctcat
     3601 gatcaagcgc gtccaaaaat tcttcgaacg cctcggtccg ggcctgatca cgggcgcggc
     3661 cgacgacgac ccgagcggca tcgcgaccta ctcgcaggcc ggcgcgcagt tcggcctcgg
     3721 catgctgtgg agcgtcttcc tcacgacgcc gctgatggtc ggcatccagg tggtgagcgc
     3781 gcggatcggc cgcgtcacgg gacatggcat cgcgcgcaac atccgcgacc actatccgcg
     3841 ctcgctgctg tggttcatcg tgtcgatcct gctcctggcg aacacgatca atatcgctgc
     3901 ggacctgggc gcgatgggcg aggcgctgca gctcgtggtc ggcgggccac agcacggtca
     3961 tgcgctcgtc ttcggcgtgc tggccgtcct gatgcagatc ttcatcccgt accgctatct
     4021 cgcgccgatc ctcaaatggc tgacactgac gctctttgcc tatgttgtcg tgctgctgac
     4081 cgtcgacatt ccctgggcga agctcatcgc ggcgacgctg atcccgccgc tcgagctcac
     4141 gagcgagtac ctgatgctgc tggtcggcgt gctcggcacg acaatcagcc cgtacctctt
     4201 tttctggcaa gcgtcgcagg aagtcgagga gcagcgcgcc acgcacggcg acgagccgct
     4261 ccgcgattcg gcgaaaggcg cgcgcgcgca tctgcagcgc atccggatcg acacgtggtt
     4321 cgggatgatc ctgtcgaacg ggatcgcgtt ctcgatcatg ctgaccacgg cggtgacgct
     4381 gaatgcggcc ggcgtcaccg acatccagac gtcggcgcaa gcggcgctcg cattgaagcc
     4441 catcgcgggt aatttcgcgt tcacgctgtt cgcggccggg atcatcggca ccggcctgct
     4501 cgcgattccc gtactcgcgg gatcggccgc gtacgcggtg gccgaagcga tgaagtggcc
     4561 gatcgggctt ggcctagagc cgctggaggc caaaggcttc tacggcatca tcacgctcgc
     4621 cacgctgctc ggtgcgggca tcgatttcac gtcgatcgat ccgatcaggg cgctcgtgtg
     4681 gtccgcggtg ctgaacggcg tcgtcgcggt gccgatcatg gccgtcatga tgcgtatggc
     4741 ggtgcggccg gacatcatgg gaccgttcgt catcaagcgc gcgttgcatt ggcttggatg
     4801 gcttgcgacg ggggtaatgg caatcgcggt cgtggcaatg ctcggatcct tgatggtata
     4861 ggcgtcggcc ggcgatccgt cggtcgtcgg ccgacaggcg tatcggcgcg gtgaacgtgg
     4921 tcgcaatggg ctttgtcgtc tatatattga tagacgcata acgcgctgca tcttccaatg
     4981 acgcggtcaa ggagaagctc atgaaaccac tcgtcaaatc cgcactcttg gccgccgcca
     5041 gcgtcgcgct cgcgggctgc gccagctacg actacgggta cggctacgcg gactcgcagc
     5101 cgtactacgg gtacgactcc ggctacagct acggatacga cgccggtccg tattcgtacg
     5161 attacggccc gtattactcc gggccttcgt actacggcgc cccgtccgtc gggctagggt
     5221 tcacgtatcg cgaccgtgat cgcgatcgcg aataccacga ccgcaattac gacgagcatc
     5281 gcaacagcga cacccggcac gacaacaact atcatcgcaa cgacgccgat ccgtggcgcg
     5341 ccggccgcga cgacccgtgg ggcgggacga acaagcccaa cgatcgcact tcacagcagt
     5401 gaaggtcggg tcccgcgacg gacgcggtac gtacggcagc ggcgaggcgc gccacgcgcc
     5461 tagccgccct ggggtcacca ccgtcgggat gcgtcgatgc cggctgacaa ctcggaacag
     5521 atcgcgcagt ggaatggcgc cctgggcgag cggtgggctg ccatgcagcg agagatcgac
     5581 cgcatcgttg tgccgttcgg cgacgcggcg ctgagggcgg cagcaccgca acctggagag
     5641 cgagtggtcg acgtcggctg cggatgcggc gacacctcga tcgaaattgc gcggagcgtc
     5701 ggtgagacgg gggcggtcct cggcgtcgac gtatcgcagc cgatgctcga ggttgctcgc
     5761 tcgcgtgctg cgttgactaa ccgcgcccac ctctcgttcc agaacgccga tgcctccgaa
     5821 gcgccgctgc cgcgcgaaac cgacctgttg ttctcgcggt ttggcgtgat gtttttcagc
     5881 gaaccctcca aggccttccg gcatttgcgc aaatcgctgc gaacgggcgg ccgctgcgta
     5941 ttcgtctgct ggcgagcacc gcgcgacaac ccctgggcga tgacgcccct ttccgctgcg
     6001 cgcatggcga tgcgggtcac gcccgcgcct cccgatcctt acgcgcctgg acctttcgcg
     6061 ttcgcggatc aggaaagatt gcacgccatc ctgtcggagg cgggcttcga tgccatccgt
     6121 gtgcagcgct tcgatgtgcc gctctcgctt ggcacgacgg cgcattccgc tgcgcagttc
     6181 gcggtgcaga tcggaccggt ttcgcgcttt gtgcgcgagg tgggcgttcc gcacctgccg
     6241 acgattctcg atgccgtcga gcgcgctctc gccccgcttg cggcagcgga cggccacgtc
     6301 agcctgaacg gttcgacctg gctcgtctcg gcgacgaatc ccggatgaga tccgcactag
     6361 cggcgaccgg gcgcgcaagc gacacgccgc gagcatcgcc gctctgtcag acagcgcgct
     6421 tcgcgaggcc actaaagttg gactaaaact ccagtcggta cagtcgcacc cacagatccg
     6481 gctccggcct cccctgcttc tttacgttga agcggtcgcc gccgtccggt gtcgtgagtg
     6541 ccgtcgtgcg ccagagagac ggccgttcca caagcatccg gcggaactct tccgtggccc
     6601 agatctgtcc gggcaggacg tggggctcga ctctgcttgc gcacaggatt gcgtttcctc
     6661 ccacgatcgt cggcgcctga tcacccacgc caggttgcat ttgcacgtcg ccatagtgca
     6721 atgccatccg caggcggggc tggcccgtcg cctgatagac gtcgtccagg atgtgccgcg
     6781 cggcctgtgc gagaaacacc ggatcttcgt ggacgatcag gagcgaatca ccggcgccgg
     6841 tttcggcgat gactgcgcgc cgggtccagt gcttgacggc ttcttcgagc gcttgccgaa
     6901 cgggtgcttc ggctccggcg tgcatcattc cgccaaaccc gtggacgtca cccttcagca
     6961 cgcacaatgc acgcacgctc gtcgcgttcg cgacggtcgc ggcctcgcgc caggggcgcg
     7021 cgtagccgac gatgttcacg cggtcgacgc attgcagata cgcgggattc acgcgtccga
     7081 tcacatccgt caggacggga tggagcaggt agtgcgtcga tgatgggagc gttccgacgg
     7141 gctcgagtgt cgcttcaccc ggccgcagga accgctgtgc ccattgtgcg cgaacgcgat
     7201 cgtggtggac atagccaagc agcccgaccc ggtagagtgc gcagaacaca tgcttctcgt
     7261 cgcccccgat cgcgatgctg tgctcgagaa acagtctttc gacgtggtcg cgggcaagca
     7321 cgttgcccgg aagtcgccgg aagagctgct cgagctccag gtcgccgatg aacggggcaa
     7381 tctcggcgag gtactcctgt gcgatctcgg tggcggcctg gtgtaccact tccttgaacc
     7441 gcacctcgct gcgccgctcc tccggccgga gcgcgaacag acgctcgccg atggacatca
     7501 aatcgcgtgg ccggagcaac gtgtgccggc agacgtactc gaagacctct tcctcctcgc
     7561 gcgtatacgc atccacgacc acggatcgtc cgagaaaggc gtcgagcggt tcggtgcgca
     7621 accgatccgc tcgcaccatc atgctgtcct tcatcaaccg cacgttgttg atgaagatct
     7681 cgcgcaggct ctggggcgag taagcaatat ccacggcgct gccgcgatac tgctggaaca
     7741 tcacggtggt tttcgcgagc cgggcatagg cttccttgcg cacggcggcg aataccttga
     7801 tgtgatggtt gatccggcgc agttgataag cgacctccac cagcccgagc tgcgcgaagt
     7861 gccacacgtt gggcgacagc tcgccggtca cgctcggatg gctcgcgagc ccttcgacat
     7921 gcttgttgaa gtactcgtcg acgccatcga tgaatatggc caccggagag ctgaccgcac
     7981 gcaggcgcgg aaccagatgg ccgtcggtgt cggcaccgca gcgctgaagc tcgcttggcg
     8041 tgaaatcgag gagtcggaca aagtgatcga tgacgctgtg aagatgctcg tcgccgagaa
     8101 gcctgttgag ggcggccccg accttgagct cgtccacggc gccaaggtgc ttgagcgtcg
     8161 caagcgaaat cgccgcgagc cagagcttcg accacggaag gggagacacc gagaaaaagg
     8221 cgaggagctc ccggctgaaa atcttgtctc cgatcggttt gtcgaggagg gcgcccatcg
     8281 gcaggcacgc cgcgcgtccc tcgcgctggt agaggattcg cttggccttg aggagaagcg
     8341 tcttgccaaa tcctttggtg ccgatgacga cgaacttgtc gtcgcggtcc gagctcagga
     8401 aggcgtcgat ctcgggcgtg cgatgaagga gggattcgtc gaaatcgtcc gccacccgga
     8461 tatcgtcggc gtcaactgtc catgcgcgca tcggccctcc gcaaagcact cagctagtct
     8521 tcgcaccgga tcgtgaatgc ggatcgcgca acagctgcgt cagcgcttcc ggattcacgg
     8581 gtttgacgag atgcacgtcg aaaccggcat ccgcggattt gcgccggtcc tcg
//
LOCUS       DASSVX010000135         3557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1621728, whole genome shotgun sequence.
ACCESSION   DASSVX010000135 DASSVX010000000
VERSION     DASSVX010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621728"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VFO53_05890"
     CDS             <1..138
                     /locus_tag="VFO53_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747959.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HET8875843.1"
                     /translation="HPLGASGGRLVTTALRQLQRTRGRYALCTMCIGVGQGIALVIER
                     V"
     gene            164..916
                     /gene="pcaH"
                     /locus_tag="VFO53_05895"
     CDS             164..916
                     /gene="pcaH"
                     /locus_tag="VFO53_05895"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HET8875844.1"
                     /translation="MSAHSNPQRKTILAGYRPADPGTQPDYLYPPYSSTVKRSPSQPL
                     VRLPWSLSEVTGPLFGFDRIMANDFDLTKQHAGDPIGERIVVSGRVLDANARPVANTL
                     VEIWQANAAGRYPHPRDNHDAPADPNFTGCGRTLTDANGRYRFVTIKPGEYPWRNHYN
                     AWRPAHIHFSLFGPALATRLVTQMYFPGDPLLEYDPMYTSIPDERARRRLVSAFDWET
                     TIPEQALGYRFDIVLRGRDETPMENDRPEALT"
     gene            913..1548
                     /gene="pcaG"
                     /locus_tag="VFO53_05900"
     CDS             913..1548
                     /gene="pcaG"
                     /locus_tag="VFO53_05900"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003189308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HET8875845.1"
                     /translation="MSASGTPEGARRAQSRASEAPADRTYLETTSSQTVGPYLHIGLT
                     WLVTDDLVGPGTSGEAIVIEGRITDADGKPVDDALIEIWQANAHGRYDHPDDARDLPL
                     EPGFKGFGRVPTDAGGAFRFRTIKPGAVPAADGSMQAPHVNVTIFMRGMLKHLATRIY
                     FPGEPANATDPLLGGVPVERRDTLIAKRAGDAVLHWNVVLHGAGETVFLEY"
     gene            1594..2934
                     /locus_tag="VFO53_05905"
     CDS             1594..2934
                     /locus_tag="VFO53_05905"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HET8875846.1"
                     /translation="MPDTLFTTDAMRAVFTARARLSRMLEFEAALARAEARVGVIPQA
                     AADAIARQCDVTRFDTKAIDAAARDAGNLAIPLVAALTRNVAAADAGAQGFVHWGATS
                     QDAIDTGLVLQLRDALELIDADLARLAGALAMQARHHAATVLPGRTWLQQALPITLGV
                     KLAAIVSALDRHRDRIASLRGRALVLQFGGAAGTLASLYDRGLAVTDELGKELGLEVP
                     DMPWHTERDRLCEVAATLGMLTATLGKLARDLSLLAQTEVGEADEPAAPGRGGSSTMP
                     QKRNPVGASIALAAAVRVPGLVATMLHAAVQEHERGLGNWPAEWETLPQIVMLTAGAL
                     DAMAEVAERLDIHADRMRANLDLTHGLVFAEAVQMALAPAIGRDAAHSLVAGACRQAA
                     TRRVHLRDILENDPRVNGVLNRDAIGRLFDPSAYLGESRAYVARALVRHPAPGV"
     gene            2948..>3557
                     /locus_tag="VFO53_05910"
     CDS             2948..>3557
                     /locus_tag="VFO53_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8875847.1"
                     /translation="MPFAQLPDVRLYYRIDGDAGSPPLLLSNSLGTSLELWEPQIGVL
                     GTRFRVIRYDSRGHGRSEVTRGPYSIEMLGQDALGLLDALAIRRAHVCGLSMGGVVAM
                     WLGVNAPQHVDRLVLANTAAKIGTAELWNARIDAVQKGGMASIAPAVLARFFSPQLLE
                     QPTPMIAQVRATLEATSKEGYAASCAAMRDADLRHALHRVRAP"
BASE COUNT          550 a         1257 c         1171 g          579 t
ORIGIN      
        1 catccgcttg gcgccagcgg cggccggctc gtcaccaccg cgctgcgcca gttgcaacgt
       61 acgcgcggcc gctacgcctt gtgcacgatg tgcatcggcg tggggcaggg catcgcgctc
      121 gtcatcgagc gcgtttgatc acacatctcg aggcagccaa cgcatgagcg cgcattccaa
      181 ccctcagcgc aaaacgatcc tcgccggtta ccggcccgcc gatcccggca cgcagcccga
      241 ctatctctat ccgccgtatt cgtcgaccgt caagcgctca ccgtcgcagc cgctggtgcg
      301 gctgccgtgg tcgctgtccg aagtcaccgg cccgcttttc ggcttcgacc ggatcatggc
      361 caacgatttc gatcttacga agcagcatgc gggcgatccg atcggcgagc ggatcgtcgt
      421 gagcggtcgg gtgctcgacg ccaacgcccg gcccgttgcg aacacgctgg tcgagatttg
      481 gcaggccaat gctgccgggc gctacccgca tccgcgcgac aatcacgacg cgcccgccga
      541 tccgaacttc accggctgcg gacgcacgct gaccgacgcc aacggacgct accgcttcgt
      601 gaccatcaag cccggcgaat atccctggcg caatcactac aacgcgtggc gcccggcgca
      661 cattcacttc tcgctgttcg ggcctgcact cgccactcgc ctcgtcacgc agatgtattt
      721 ccccggcgat ccgctgctcg aatacgatcc gatgtacacg agcattcccg acgagcgggc
      781 gcgccgccgg ctcgtctcgg cgttcgactg ggagacgacg atccccgagc aggcattggg
      841 ctaccgcttc gacatcgtgc tgcgcgggcg cgacgagacg ccgatggaaa atgatcggcc
      901 ggaggcgctc acatgagtgc cagcgggacg cccgaaggcg cgcgccgcgc gcaatcccgg
      961 gccagcgaag cgccggcaga ccgtacgtac ctcgagacga cttcttcgca aaccgtcggg
     1021 ccgtatctgc atatcggcct cacttggctc gtgaccgacg acctcgtggg gcccggcacg
     1081 tcgggcgagg cgatcgttat cgagggacgc atcaccgatg ccgacggcaa gccggtcgac
     1141 gatgcgctga tcgagatctg gcaggcgaat gcgcacggcc gttacgatca tcccgacgac
     1201 gcacgcgatc tgccgctcga gcccggattc aagggcttcg gccgcgttcc caccgatgcg
     1261 ggcggcgcct tccgtttccg cacgatcaag ccgggcgccg ttcccgcggc cgacggctcg
     1321 atgcaggcgc cgcatgtcaa cgtcacgatc ttcatgcgcg gcatgctgaa gcacctcgcg
     1381 acgcgaatct actttcccgg cgagccggcc aacgcgaccg atccgctgct gggcggcgtg
     1441 ccggtcgagc gtcgcgacac cctgatcgcg aaacgcgcgg gtgacgccgt tctgcactgg
     1501 aacgtcgtgc tgcacggtgc cggcgaaacc gtgttcctcg agtactgacg cttgcaacga
     1561 tgatcctcgc ggcctgctga gcggcgcccg cccgtgcccg acacgctctt caccaccgat
     1621 gcgatgcgcg cggtctttac cgcgcgtgcg cggctttcgc gcatgctcga attcgaagcg
     1681 gcgctcgcgc gtgccgaagc gcgcgtcggc gtcatcccgc aggccgctgc cgatgcgatc
     1741 gcgcggcagt gcgacgtgac gcgcttcgat acgaaggcga tcgacgcggc cgcgcgcgac
     1801 gcgggaaatc tcgcgatccc gctcgtcgct gccttgacgc gcaatgtcgc ggcggccgac
     1861 gccggcgcgc agggtttcgt gcattggggt gcgacgagcc aggacgcgat cgacaccggc
     1921 ctcgtgctgc aacttcgcga cgcactcgag ctcatcgacg ccgacctcgc acgtctcgcc
     1981 ggtgcgctcg cgatgcaagc ccggcaccac gcggcgaccg ttcttcccgg ccgcacctgg
     2041 ctgcagcagg cgttgccgat cacgctcggc gtcaagctcg ccgcgatcgt gagcgcgctc
     2101 gaccgccatc gcgatcgcat cgcatcgttg cgcggccgcg cgctcgttct gcagttcggc
     2161 ggcgccgccg gcacgctcgc atcgttatac gaccgcggcc ttgccgtgac ggacgagctc
     2221 ggcaaggagc tcggcctcga ggtgcccgac atgccatggc ataccgagcg cgatcggctc
     2281 tgcgaggtcg ccgcaacgct tggcatgctg acggccacgc tcggcaagct cgcccgcgat
     2341 ctttcgcttc tcgcgcaaac cgaagtcggc gaggccgacg agcccgcggc accgggacgc
     2401 ggcggttcgt cgacgatgcc gcagaaacgc aatcccgtcg gcgcatcgat cgcgcttgcc
     2461 gcggccgtcc gcgtccccgg cctcgtcgcg acgatgttgc acgccgcggt acaggaacac
     2521 gagcgcggac tcggcaactg gcccgcggaa tgggaaacgc tgccgcagat cgtcatgctg
     2581 accgcaggcg cgctcgacgc catggcggaa gttgccgaaa ggctcgacat ccacgccgac
     2641 cgcatgcggg ccaaccttga tctcacgcac gggctcgtct tcgccgaggc ggtgcagatg
     2701 gcgctcgcgc cggcgatcgg ccgcgatgcc gcgcattcgc tggttgctgg cgcctgtcgg
     2761 caggccgcga cacggcgcgt tcatttgcgc gacattctcg agaacgaccc gcgcgtgaac
     2821 ggcgtgctga accgcgacgc gatcggcagg ctcttcgatc cgagcgctta tctcggtgag
     2881 agtcgcgcgt atgtcgctcg ggcactcgtt cgtcatcccg cgcccggggt ttgagggaac
     2941 atacgcgatg ccattcgcac agcttccgga tgtacgtctc tactaccgca tagacggcga
     3001 cgccggctcg ccgccgctgc tgctgtccaa ttcgctcgga acgtcgctcg agttgtggga
     3061 gccgcagatc ggtgtgctcg ggacgcgttt tcgcgtgatc cgctacgaca gccgcggtca
     3121 tgggcgctcg gaggtgacgc gcggcccgta ctcgatcgag atgctcgggc aggacgcgct
     3181 cgggctgctc gatgccctcg cgatccggcg tgcacacgtg tgcggtctgt cgatgggcgg
     3241 agtcgtcgcg atgtggctcg gcgtgaatgc gccgcagcat gtcgatcgcc tcgtgctcgc
     3301 caatacggca gcgaagatcg ggaccgcgga actatggaat gcgcgtatcg acgcggtgca
     3361 gaagggcggc atggcgtcga tcgcgcccgc ggtgctcgcg cgctttttct ccccgcagtt
     3421 gctcgagcag ccgacaccga tgatcgcgca ggtgcgtgcg acgctggagg cgacgtcgaa
     3481 ggagggctac gccgcttcgt gcgcggccat gcgcgacgcc gacctgcgcc acgcgctgca
     3541 ccgcgtgcgc gcgccga
//
LOCUS       DASSVX010000136         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_888558, whole genome shotgun sequence.
ACCESSION   DASSVX010000136 DASSVX010000000
VERSION     DASSVX010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888558"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VFO53_05915"
     CDS             <1..693
                     /locus_tag="VFO53_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET8875848.1"
                     /translation="TIEDPIEYLFDHRQSMVNQREIGIDTHNYHMALQSALREAPDLI
                     MIGEIRDKETMQAALLHTLTGHLCLSTIHANNSYHALSRIINLFPHEARSAVLSDLSI
                     GLRAIVSQRLVRNVEGGQQPAVEILLNTSLIAELIKSGEFTQIKEAMEQSLYPGSQTF
                     EQALCKLYLDQRISYDEAMVASDSATNLAWLINQNSPSSRIDSSRDEPTLNVRPAADF
                     ASMTINPELLER"
     gene            779..1933
                     /gene="dapE"
                     /locus_tag="VFO53_05920"
     CDS             779..1933
                     /gene="dapE"
                     /locus_tag="VFO53_05920"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HET8875849.1"
                     /translation="MPPQSDPAFRTGAVLDLTRALIARRSLTPDDAGCQALLAERLAP
                     LGFRCDTLSVNGVTNLWARRGERAPLVCLAGHTDVVPTGPLADWTSDPFTPTVRDGYL
                     YGRGAADMKSSLAAFVVAVERLLARHDPAGSIALLITSDEEGPSVDGTAQVVERLARA
                     GTAIDYCIVGEPSSVERLGDMIKNGRRGTLSGTLIVKGVQGHVAYPHLARNPIHAIAP
                     ALAELARMHWDDGNEYFPPTSFQCSNVHAGTGATNVIPGALELLFNFRYSTQSPRESL
                     AARFEEVLQRHNVDYALVWTAHGAPFLTPQGRLVDIATQAIRDVAGVVPALSCEGGTS
                     DGRYIARICPEVVEIGPVNATIHKLNERVAVADLDRLAEIYTRMLASLLG"
     gene            1930..2826
                     /gene="prmB"
                     /locus_tag="VFO53_05925"
     CDS             1930..2826
                     /gene="prmB"
                     /locus_tag="VFO53_05925"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET8875850.1"
                     /translation="MNAPPELETLRDWLRFAVSRFAHAGLSFGHGFATACDEALYLLL
                     HTLHLPLDHAEPFLDAKLTHAERSELAALLTRRIEEHVPAAYLTREAWLGDFRFHVDE
                     RVLIPRSFIAELLPDGLAPYVADTASVKSALDLCTGSGCLAVLLAHAFEAADIDAADI
                     SSDALAVAQRNVSDYGLAGRINLIRSDLFDNLPGKSYDLIISNPPYVTTVAMEALPRE
                     YRHEPALALAGGDDGMDAVRTILREAPRFLQPGGLLVVEVGHNREAAESAFPRMPFVW
                     LSTASSADSVFLLKRDDIVAGR"
BASE COUNT          464 a         1046 c          918 g          491 t
ORIGIN      
        1 acgatcgagg acccgatcga atacctgttc gaccaccgcc agagcatggt caaccagcgc
       61 gagatcggca tcgatacgca caactaccac atggcgctgc aaagcgcgtt gcgcgaagcg
      121 cccgacctca tcatgatcgg cgagatccgc gacaaggaaa cgatgcaggc ggcgctgctg
      181 catacgctga ccgggcacct ctgcctgtcg acgatccacg cgaacaacag ctatcacgcg
      241 ctgtcgcgga tcatcaacct gtttccgcac gaggcgcgct cggccgtgct gtccgacctg
      301 tcgatcgggc tgcgtgccat cgtgtcgcag cggctggtgc gcaacgtcga aggcggccag
      361 cagccggcgg tggagatcct gctcaacacg tcgctcatcg ccgagctcat caagagcggc
      421 gagttcacgc agatcaagga agcgatggag cagtcgctgt accccggatc gcagacgttc
      481 gagcaggcgc tctgcaagct ctatctcgac cagcgcatct cgtacgacga ggcgatggtc
      541 gcgtccgact cggccacgaa cctcgcatgg ctcatcaacc agaactcccc gtcgtcgcgg
      601 atcgactcct cgcgcgacga accgacgttg aacgtgcgcc ccgcggccga tttcgcgagc
      661 atgacgatca atcccgaact gctcgaacgc tgatccatgc ggcacctggt gctcgcgccg
      721 cgggttttgc gaaagtgacg ctcccgctcg cggcgaaacc gggagtatcg tgtcgggcgt
      781 gccgccgcaa tccgatcccg cttttcgcac aggcgccgtc ctcgacctca cgcgcgcgct
      841 gatcgcgcgc cgctcgctca cgcccgatga tgccgggtgc caggcgctgc ttgccgagcg
      901 cctcgcgccg ctcggcttcc gttgcgacac gctgagcgtc aacggcgtca ccaatctctg
      961 ggcgcgtcgc ggcgagcgcg cgccgctcgt ctgccttgcc gggcacaccg atgtcgtgcc
     1021 gaccggtccg ctcgccgact ggaccagcga tccgttcaca ccgaccgtgc gcgacggcta
     1081 tctctacgga cgcggcgcgg ccgacatgaa gtcctcgctc gccgcattcg tggttgccgt
     1141 cgagcgcctg ctcgcgcggc acgatcccgc cggctcgatc gcgcttttga tcacgtccga
     1201 cgaggaaggt ccgtcggtcg acggcaccgc gcaggtggtc gagcggcttg cacgcgccgg
     1261 cacggcgatc gactactgca tcgtcggcga gccatcgtcg gtggagcggc tcggcgacat
     1321 gatcaagaac ggacgccgcg gcacgctctc ggggacgctg atcgtcaagg gcgtgcaggg
     1381 ccacgtcgcc tatccgcatc tggcacgcaa cccgatccac gcgatcgctc ccgcccttgc
     1441 ggagcttgcg cgcatgcatt gggacgacgg caacgaatac tttccgccga cgtcgttcca
     1501 gtgctcgaat gtccatgccg gcaccggcgc gacgaacgtc attcccggcg cgctcgagct
     1561 attgttcaat tttcgctact cgacgcagag tccgcgcgag tcgctcgccg ctcgcttcga
     1621 ggaggtgctg caaaggcaca acgtcgatta cgcgctcgtc tggaccgcgc acggtgcgcc
     1681 gttcctgacg ccgcagggcc ggctcgtcga cattgcgacg caggccatcc gggacgtggc
     1741 cggtgtcgtg ccggcgctct cgtgcgaggg cggcacgtcg gatggccgct acatcgcacg
     1801 catctgcccg gaagtcgtcg agatcgggcc cgtcaacgcg accatccaca agctgaacga
     1861 gcgcgtcgcc gtcgccgacc tcgaccgcct cgccgagatc tacacgcgca tgctcgcgag
     1921 cctgctggga tgaacgcgcc gcccgagctc gagacgctgc gcgactggct gcgctttgcc
     1981 gtgtcgcgct tcgcgcacgc aggcctttcg ttcgggcatg gcttcgccac cgcatgcgac
     2041 gaagcgctgt acctgctgct gcacacgttg cacctgccgc tcgatcatgc ggagcctttt
     2101 ctcgacgcga agctcacgca cgccgagcgc agcgagctcg cggcgctgct gacccggcgc
     2161 atcgaggagc acgttcccgc cgcgtacctg acgcgcgagg catggctcgg tgatttccgc
     2221 tttcatgtcg acgagcgggt gctcattccg cgctcgttca tcgccgagct gttgccggac
     2281 ggcctcgccc cgtacgtcgc cgatacggcg tcggtcaaat cggcactcga tctttgcacc
     2341 ggctcgggct gcctcgccgt gctgcttgcg catgcgttcg aggcggcgga catcgacgcc
     2401 gccgacatct catcggacgc gcttgcggtc gcacagcgca acgtcagcga ttacggactc
     2461 gcgggccgca tcaacctgat ccgctccgac ctcttcgaca acctgcccgg caagtcgtac
     2521 gacctcatca tcagcaatcc gccgtacgtg acgacggtgg cgatggaggc gctgccgcgc
     2581 gagtatcgcc acgagccggc gctggcactc gccggcggcg acgacggcat ggacgcggtg
     2641 cgcacgatcc tgcgcgaggc gccacgcttc ctgcagcccg gcggcctcct cgtcgtcgag
     2701 gttggacaca atcgcgaggc cgctgaatcg gcgttcccgc gcatgccgtt cgtgtggctt
     2761 tcgaccgcgt cgtccgccga cagcgtattc ctgctgaagc gcgacgacat cgtcgccggg
     2821 cgttagtggc ctgtcccggg tcacgcacgt tcacaaccga ttcccccgtc acaggccacc
     2881 agccggccgc gccggcctgc gcgatcattg cgcaaggcc
//
LOCUS       DASSVX010000137        23011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1621827, whole genome shotgun sequence.
ACCESSION   DASSVX010000137 DASSVX010000000
VERSION     DASSVX010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 23011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23011
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621827"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFO53_05930"
     CDS             complement(<1..433)
                     /locus_tag="VFO53_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HET8875851.1"
                     /translation="MAAKDVRFGENVRNKMVNGVNVLANAVKITLGPKGRNVVLERSY
                     GAPTITKDGVSVAKEIEFKDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQSIIAE
                     GMKYVAAGMNPMDLKRGIDKAVIAIVEELKKVSKPCSTSKEI"
     gene            complement(472..759)
                     /locus_tag="VFO53_05935"
     CDS             complement(472..759)
                     /locus_tag="VFO53_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET8875852.1"
                     /translation="MKLRPLHDRIIVKRLEEERKTTGGIVIPDTAAEKPSMGEVIYAG
                     PGKTDDNGKLIPMGVKAGARVLFGKYSGQEFKLEGQDLLHMREDDVIGIVD"
     gene            complement(942..1235)
                     /locus_tag="VFO53_05940"
     CDS             complement(942..1235)
                     /locus_tag="VFO53_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875853.1"
                     /translation="MNDKDRVTLDVTREFQRRMAAIIDAVQSGVWEAGVHDLLGHATD
                     YAFGQQRGLQTLVLKTTNRSAYVRLTWDTVMGTSDGDRQLVDAAVASAIRELS"
     gene            1310..2578
                     /locus_tag="VFO53_05945"
     CDS             1310..2578
                     /locus_tag="VFO53_05945"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8875854.1"
                     /translation="MRPDSHAQTGPLELAGLASRLVDRGLKGLAVAEQVDAFCRDLFD
                     AGFRASRFNMSIGTLHPRHGAQSYVWRPDGIATELHPRRRNAEEDEAYQRSPIHHLRT
                     SGDAALRRRLDASHADDFPILVDLREEGMTDYAATIVRFSEASNGDARAERVRAAFDR
                     PDPLQGIFFSCATDDPGGFNDEHLRQVTELLPYLALGVKSRSTFDVAQTLLETYLGPD
                     AGSRVLTGEIDRNSVRNIQAVIWLCDLRGFSRTASRVTQDELIELLDVYLEVMARPVL
                     NNRGQILKFMGDGFLATFDLAKRSREAVCADAVNAAQQLLGTLPALNSERRASSKTTL
                     DLGVAIHIGDVMYGNIGTRERLDFTVVGSAVNEVSRMEGLCRPLGRNVLVSRTVAEAA
                     AASRGKLLSLGVHALRGIREPQELFTIGDA"
     gene            2878..6765
                     /locus_tag="VFO53_05950"
     CDS             2878..6765
                     /locus_tag="VFO53_05950"
                     /inference="COORDINATES: protein motif:HMM:NF021665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HET8875855.1"
                     /translation="MPQHGPLRTEDAADTRAVLPLQQDGADLTVTTPCIVKAGTYKFR
                     DVHILDQGRLIFTDARIDFWAANILVEDGGSLVAGSPQAPIGTDGGVVTIHLWGADQT
                     GVDPKKQGIGIACIGDSVGRCGVPDEIWQSNVDAMGMPVPVSKARKIASLAAARQYPG
                     VTDDYFYAYQPLTFDGGSDATHPVGYFGSKVIGVSYGGTLQLFGRKGATYATPECGDA
                     PTTSGTSWARLDRPAPKGTSLLTVDRPMSLQAGDRIVVTTTDYLPGHSEQLTVATDVA
                     CGTAIAVREALAYAHYGARFPLTVVPEDVGLDPQLRAAGAETRAAVGVLTRSIRIVSA
                     GDTMDDDFPPEPPAGSTTPGYYFGGHLIVRQGFKTFQLQGVELHQMGQGGRIGHYPVH
                     FHHARRTPANTFVRDSSIDDSMTRWIVLHGTQDVKLERNVGYRSIGHGFYLEDGTEIN
                     NVLTANLGIFARAAVDNVQNPRKVPGILAAPDLHATTGEAVPYRSDYDHPTIFWIMNG
                     WNDFRDNMAVGAGTCGVCYWLLPAINSTMSRDMKWSSYASMQSTLDRAGMAPLKSFVG
                     NGCSAAMTSFQTITATEACQGVGPGDAASPHPLNLLPVKNPLATPSKPKEAAADYYPS
                     LGDGGHFPTKCAQEDCSSRPVRCSAGNAADCMVTILDHYTTSFNWAAFNFAAIWLRPQ
                     WYLVTDSVITDSQQAGLTIVTGGGYSDSDVIPGHWALVRKSVFIGNTQDDNPFASNGG
                     PFNPGALRCAMDANGSRPGNFCLSIDEGVSHQVSNFGMYQRLFSVYDGPAFQDSNAYL
                     HIKTRRIDDCQPFVDTVNKAGRCDPVDLSPGKPRQSAWLAGFVAGLPKAVGAGGPFCY
                     MPNAAIGWKQPNGFYYPPAFHSANLFFNDVDIRHFVVSPLFVEGTYDTDIARVADKYC
                     IWNSQLFTGFAGNDRQTVLNDDDGTLTGYRKTTIINQDAFFAAPVDATQCDSDDTSRT
                     SPYEYVTTVLYPACVLNGTCARPPDVAPKPPDPHWNDGDWNRSCTSEKCYGIPLWRQD
                     LMPVADKGAAKSIRMMGQETAQRSSLTVNHGTYYFDTSVSKETQLMQGCTTTDPANPC
                     VINVFKPNQTYYLFLIFAKATTEQTYRFYVGDDTDFDPASIELVQAQIGRNPVKYETV
                     GPLPAGRAHWLDNDRAKAHGVVEVDLMLSDLPDVATLFTAAKKNKCQPSTYCKWNDAT
                     EQCVDADGSDAVCKWAIADQDCPAGGCFGIRFTLPSGFATRAANDARPDPRPVALCVP
                     KDAPWDVSLDALKVSDGVCPKEPDRQPLDYCAQ"
     gene            complement(6832..8526)
                     /locus_tag="VFO53_05955"
     CDS             complement(6832..8526)
                     /locus_tag="VFO53_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8875856.1"
                     /translation="MQAPNSEWLIPGGPSAGIASRLAQMFPALTDAEIARMRRFGALR
                     RFPAGARLYTAGETIPGMLIVLKGTVTISQRDGLGHVAPIVRQGPGQFLAELAQLSGG
                     AAVVDAVADEEVEALLISTERLRALIVADAELGERITRALILRRVGLIEQEAGPALIG
                     TPQLPDLLRLENFLRRNGQPHHAIDPSQEPAAAALFAQYGGASGDVLVVCPNGGVLLT
                     PSEDALGRCLGMLDSAVHDERFDVTVVGAGPGGLATAVYAASEGLHVIVVECRAFGGQ
                     AGASARIENYLGFPTGISGQALAGRAFVQAQKFGAEILIPAQAMSLDCHDAAHDGEFR
                     IRLGDERTLRSRTVVIASGARYRRPAVPGLAEFEGRGVWYWASALEAKMCAGEEVAIV
                     GGGNSAGQAAVYLARHAARVHLLVRRESLAATMSRYLIDRIAATPAIDVHYNAELSHL
                     HADAGARLAAITCRDNRSGAEQRHPVRNVFLFIGADPETEWLRSCGVALDEHGFVLTG
                     EAASRASGRRAPASLESSVPGVFAVGDVRAGSVKRVGGAIGEGAAAVGLIHAHLSG"
     gene            8676..10184
                     /locus_tag="VFO53_05960"
     CDS             8676..10184
                     /locus_tag="VFO53_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HET8875857.1"
                     /translation="MTSAAAFRFDNSFARELEGFYVARRPAVVPAPRLLYLNEPLADE
                     LGLGVSALDGEQSAAIFAGNTLPDGAQPLAQAYAGHQFGGFSPQLGDGRALLLGEVID
                     RHGKRRDIAFKGSGRTPFARGGDGKAAVGPMLREVLVSEAMHALGIPTTRALAVVATG
                     EPVYREQVLPGAVLTRVAASHIRVGTFEFHAARGDMESVRKLADYTIARHDPGLAASP
                     QRFLALLQHVAERQAALIAQWMNVGFIHGVMNTDNMTISGETIDYGPCAFIEAYDPAA
                     VFSSIDEQGRYAFGNQPDIACWNLARLAETLLPLLSEDQQEAIAMANEVIARFPAQYR
                     RHLLCGQRAKLGLRRAEPADDTADTALADDWLSLLHAGRADFTLAWRRLADAAGGNEA
                     PLRALFADAPRLDAWLARWGARCASEAGGETPGTTLERADAMRRVNPIVIARNHRVEE
                     ALAAASDRGDLAPFRRLLDAVSHPYVETVEFAAYAEPAPAAVTACYRTFCGT"
     gene            complement(10190..10669)
                     /locus_tag="VFO53_05965"
     CDS             complement(10190..10669)
                     /locus_tag="VFO53_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875858.1"
                     /translation="MQARDRTRHFITMRKGNAMLSFHMLRPPSAPLAARCGVRTQCLA
                     IIALAAAFPIAAYAAHTEGATQAPEDSLLIKMLNAQIHALDGYERCLEGEGLPAPCEP
                     PRPLQLPARELTADRGAHRDALIGSAERSLKEAELHAIRTHARCMKTRQSGTCGPAP"
     gene            complement(10718..11008)
                     /locus_tag="VFO53_05970"
     CDS             complement(10718..11008)
                     /locus_tag="VFO53_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875859.1"
                     /translation="MDRRHDRLVWAVTVLAFAILVSAAASHLHVVADLDEGCAICAAV
                     GIGKLEGPAPNLVAPVPLAVNGFRLADPGPPSLAQSAGVVVLPPSCGPPGIA"
     gene            11091..13298
                     /locus_tag="VFO53_05975"
     CDS             11091..13298
                     /locus_tag="VFO53_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET8875860.1"
                     /translation="MNSIGKTRCVRFVFIACVASLMGAAAAADVHRAPAPPAKAPVRD
                     VTDTHFGVTVDDPYRYLEDLKDPDVAAWIKSQAHYTREVLDRVPHRQALYEEIAKYGD
                     SASARVTHVQLSGSYVYYEKRRAADNLPKLYVRRGLRGKERLLVDPERLKAPDGAHYA
                     IDYYAPSPDNRYVAYGISLGGSEQSVLHVLEVATGRETGDVIDRANYASPSWLPDGRL
                     LYSRLQKLAPGAPVTDKYQNQRVYLHRLGSDPEQDVAVFGAGVAAQIDVRPVELVFAG
                     VAPGSDYVVALAVNGTQREVRIWTARLSTLDGAKTRWTGVADTGDQVTDASVAGNTLF
                     MVSHKDAPRFKVLRLPLSAPDIAKADVVVPPAESIVTGIAAASDALYVRRMNGGISEL
                     LRLGFDAAGTAKAIKLPYDGDIDALAADPRMPGLLFTMGGWTRFGSVFAYAPKSGRIA
                     DTGLQPQGPYDNPRDLVSEEVKATAADGTEIPLSIVYRKGLQHDGGNPTIVWGYGAYG
                     ISQTPVYAPTLLPWYERGGIFAVCHVRGGGEYGEDWYKAGFQATKPNTWRDAIACAQW
                     LVDHRYSSPAKLSIRGGSAGGILVGRAITERPDLFAAAIDDVPVSDTLRMEFSANGVP
                     NIPEFGSVKTEAGFRALLEMSPYAHVKDGVAYPAVLLTTGFNDPRVDAWEAAKMAARL
                     QAATSSGKPILLRVDYDAGHGFGTSKKSAYQLAADRFAFLLWQLGVAGYQPPS"
     gene            13483..13938
                     /locus_tag="VFO53_05980"
     CDS             13483..13938
                     /locus_tag="VFO53_05980"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET8875861.1"
                     /translation="MDEEIRRKIQSLLDQHRAMTIATMRPDGWPQATTVGYANEGLTL
                     YFLCGPESQKAANLARDDRVSLTIDDDTPQVMEISGLSMAAHAQVVDDPAEAEKALRL
                     LMLRYPEQKSMPFPMPTSADVRIFRLVPMVVSVLDYSKGFGHTDLVSCQ"
     gene            14237..16933
                     /locus_tag="VFO53_05985"
     CDS             14237..16933
                     /locus_tag="VFO53_05985"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET8875862.1"
                     /translation="MRGRSRVATIVAPGSGCALWFMVLDKSVGVDTEKRTVAQARAAH
                     ADGRRGVARAGAMNRPPGLDSEAARARLARQGPNELPRSAPRSFIANTAHALAEPMFG
                     LLVVAASIYFALGDRVEALFLAASVVVMIAITLYQEQKTARVLRALRDLASPRALVIR
                     DGVGRRVPGREVVVDDVLVVREGDRVAADGRLIEADDLHVDESLLTGESAPVAKKASS
                     SSPPASLESNACNVYGSTLVVRGQGIARVTATGPESEIGRIGRSLEPLATEATVLQVE
                     MRRLVLVFSVVALLVCAVLVGVYVLTRGGWLNGVLAGLTLAMAILPEEFPVVLTLFLA
                     LGAWRMAQVQVLTRRIAAIEALGAATVLCVDKTGTLTQNRMAVQRIYSAGQRIDVASA
                     SAGALPPLFAEAVHVSVLASEPHPFDPMERALLDVDPVTDAPALELVRRYPLTTGRLA
                     VTHVWRDRDATHVMVAAKGAPECIVELCGLTADHAAGVLIEAAAMAASGLRVLGVAKA
                     EHDATSLPEDPKDLHLDFLALIGLADPLRPAVPQAMRECRAAGVRVIMITGDFPVTAV
                     AIAREAGLAEQPAVMTGRELDTMSDDALCARLSTVDVFARVVPSQKLRIVNSLKMRGD
                     IVAMTGDGVNDAPALKAAHIGIAMGRRGTDVAREAAALVLLDDDFTSIVRAIRQGRRI
                     FDNLRKAMSYLVSVHVPIAGLGLVPVLLGGPLVLFPAHIVFLEFVIDPVCSIAFEAEP
                     AEPDVMRRPPRSARRRLLGGLPFALAVLEGGVALAFTVAVYAISLASEGGETRTRLLS
                     FTAVIIANLSLIFFARAGGRRIRRHVAAANRSLWLIVAGTLAAYGVVVAVAPFREVFR
                     MAAPTAADARLLVATTLLLWALLATLNLAYGTLAKRSGQHAA"
     gene            17032..17595
                     /locus_tag="VFO53_05990"
     CDS             17032..17595
                     /locus_tag="VFO53_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET8875863.1"
                     /translation="MFKHILVALDGSAASNAGFKSALQLASDQHASVTGVSVIDDAAV
                     RANFDGAYLAASYVDTLYDSLCENGRAILAKAESAARSAGVAFEPLLVESRGQTVAHA
                     ILQQARKAKVDVIVLGTHGRRGLSRMLMGSDAEAVVRESRVPVLLVRSGERATRRRST
                     AAMPAAASSRKRGTTAQPKPRPSRTQA"
     gene            complement(17663..19282)
                     /locus_tag="VFO53_05995"
     CDS             complement(17663..19282)
                     /locus_tag="VFO53_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET8875864.1"
                     /translation="MDADVIVIGAGISGALVADRLAATGVKVLILEAGPRIQREQAVA
                     QFFNAAIKVPECAYPNTAYAPHPRSDDLDFYYVQTGRDKFKSTYLRQVGGTTWHWLGT
                     CLRLVPDDFRLATRFGRGVDWPIDYSALEPWYGRAEEAIGVAGDSSADLGSPRSSPYP
                     MPRIAPSYLDAAITKAFEESGLNVRSTPQGRNSVVRDDRPPCCGNSSCIPVCPIQAKY
                     DATVHVARAEKGGARLLARCVVHRIEIGGDGNVAAVRYKRPDGSDHRATAKVYVLAAH
                     AVEIPKLLLMSRGPNAPNGVANSSDQVGRNLMDHPIQLSWALSESPVYPYRGPLSTSG
                     IENTRAGDWRAMRPAFRIEIGNDGWSWPTGAPASDARALAGKGLRGAALQKALYERTA
                     RQFRFATLTEQLPDPQNRIALDPIEHDALGLPRPRIAYRIDDYTKAGLAEGRRIHEQV
                     FKRLNATEIAHNADQDFQGAGHVMGTARMGTDPKRAVVDGNLRAFDHPNLFIEGSAVF
                     PTGGAANPTLTIAALALRSVDAIRAALTATR"
     gene            complement(19285..19737)
                     /locus_tag="VFO53_06000"
     CDS             complement(19285..19737)
                     /locus_tag="VFO53_06000"
                     /inference="COORDINATES: protein motif:HMM:NF023737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar dehydrogenase complex small subunit"
                     /protein_id="HET8875865.1"
                     /translation="MDVDRRRVLQSIVGATLAALGAGTPAAAPLSVANFGVVSAALTG
                     YPAPSAADATKLWAAFGTPARRAALTRLARVVDAAGSADLDAALRVQGLDDIADELVA
                     AWYSGVVTQGATPRVVLYAGAYMWSAMSFTKPMGQCGGATNYWADPPT"
     gene            complement(19783..20715)
                     /locus_tag="VFO53_06005"
     CDS             complement(19783..20715)
                     /locus_tag="VFO53_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8875866.1"
                     /translation="MNAVFVDATPALASLADELFRVDGIAFSVNRDPEVRPESLPGLL
                     AGADVAIIDHTPLPTAIAKQCVGLRDVIFLGTGARSYMSVEELAALGIQVHTIKGYGD
                     TAVAECTIALMWAAARGVARMDREMRGGTWLRTEGIELTGRTLGLVGFGSIAAEVARI
                     ALGGGMRVIAWNRSPKAHAGVTFVPLERLLAESDVVSMHLLLNDETRGFLSRARIAAM
                     RPGAILVNTARGALVDEAAMIDALRDGRLRHAGLDVYDVEPLPAGHPLTTVPNVTLSA
                     HSAFRTPEASRNVVAAAREHCRRIVGARVAEGLR"
     gene            complement(20801..22498)
                     /locus_tag="VFO53_06010"
     CDS             complement(20801..22498)
                     /locus_tag="VFO53_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET8875867.1"
                     /translation="MRYLALASDYDNTLAADGRVSDATRAALGRLRASGRRAILVTGR
                     RLDDLLRVCDCAELFDYVVAENGALVHDPRSRDTTLLADPPPEQFIAALRRRGVQPLE
                     VGKVIVATHAPQQTQVMETIRELGLELQVIFNREAVMVLPAGINKASGTKVALRNLGM
                     SPHELVAIGDAENDHSFMHLAECPVAVANAVDSIKRLAAFVTAGAAGQGVVELIDELI
                     ENDLGPIDARLTRRHVALGTRPDGRTVWLPPYGNNVLIAGPSGGGKSTLASGLLERFA
                     ERFYQVCVIDPEGDYVSLQMLVTIGDQQRAPGIAEIMAILRDPEVHANVNLLGVPLAD
                     RPARFAELLPHLRAMRMRTGRPHWLLIDEAHHLLPRSWGHPTERFNESILVTVHPERL
                     APSILAWVDVVIAVGASPDRTLREFAAASNRAADAVHALHVDNDQVACWFVHAAEGPF
                     AMSAIPGRAERIRHHRKYAEGDVRYQSFYFRGPTGKHNLKAQNLSVFCHIAEGIGEET
                     WLFHLRRNDYSRWMRDVIKNRDLAAAVEDIERRIDLPPAQTRALVCAAIETRYTLPQ"
     gene            complement(22572..>23011)
                     /locus_tag="VFO53_06015"
     CDS             complement(22572..>23011)
                     /locus_tag="VFO53_06015"
                     /inference="COORDINATES: protein motif:HMM:NF044721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HET8875868.1"
                     /translation="DWNCLERHRDIYRFTRGMIAFRRAHPVLCEERFYTDADMRWFDP
                     DLGLPAWADPRERRLACLIHEAEGSALYLMFNAGADPAPFRVPPASDGARWQLAVETG
                     AESPRDLFEPGEEPPVDAARPCRVAPRSAVVLVQRNASWFAER"
BASE COUNT         3891 a         7650 c         7555 g         3915 t
ORIGIN      
        1 cgatttcctt gctggtcgag cacggcttgc tcaccttctt cagctcctcg acgatggcga
       61 tgaccgcctt gtcgatgccg cgcttcaggt ccatcgggtt catgccggcg gcaacgtact
      121 tcatgccttc ggcgatgatc gattgcgcca gcacggtggc cgtcgtcgtg ccgtcaccag
      181 cgacatccga cgtcttcgac gcgacttcct tcaccatctg cgcgcccatg ttctcgaact
      241 tgtccttgaa ttcgatttcc ttcgcgaccg acacgccgtc cttggtgatc gtcggtgcgc
      301 cgtaactgcg ctcgaggacg acgttgcggc ccttcgggcc gagcgtgatc ttgacggcgt
      361 tggcgaggac gttcacgccg ttcaccatct tgttgcgtac gttctcgccg aagcgaacgt
      421 ctttggctgc catgagtcgt ttctcctgat gcttctgttc ggggtgggcg gtcagtcgac
      481 gatgccgatg acgtcgtctt cgcgcatgtg cagcagatcc tggccttcga gcttgaattc
      541 ctggccggaa tacttgccga agaggacgcg ggcgccggcc ttgaccccca tcggaatcag
      601 cttgccgttg tcgtcggtct tgccgggtcc ggcgtagatc acttcaccca tcgacggctt
      661 ttcggccgcc gtatcgggga tgacgatgcc gcctgtcgtc ttgcgctcct cttcgagccg
      721 cttgacgatg atccggtcgt gcaggggacg cagtttcata tctttactca cttccgattg
      781 gggttggcag cgaatcgtgt cgctgcgggg cggattgccc ctggcacggc cgccggatgg
      841 cttcagcggc ccgggggaat acgatggaag atgctgcctg tggtgccagc accacggacc
      901 gccaatatat gggtccgcaa ttgcaacaat gcaaccccgc gctagctcaa ttccctgatg
      961 gcgctcgcca ccgccgcgtc cacgagctga cgatccccgt cgctcgttcc catgaccgtg
     1021 tcccaggtca gtcgtacgta cgcggatcgg ttggtggttt tcaacaccag tgtctgcaga
     1081 ccccgttgct ggccgaaagc gtagtcggtg gcatggccca gcagatcgtg cacgccggcc
     1141 tcccacacgc cgctttggac cgcatcgatg atcgcggcca tgcgacgctg gaattcccgc
     1201 gtcacgtcga gcgtgacccg gtccttgtcg ttcatcgggg aggcacccat ggttccatta
     1261 tgatagacga gacgtcgcaa cgcatgtgca ctcgcacacg caggaaacca tgagaccgga
     1321 tagccacgcg cagacgggcc cgctggagct tgccggcttg gcatcgcgtc tggttgaccg
     1381 cgggttgaag ggactcgcgg ttgccgagca ggtcgatgcc ttttgccggg acctcttcga
     1441 cgccggattt cgcgcgagtc gattcaacat gtccatcggc acgctccatc cgcggcacgg
     1501 cgcgcagagt tacgtgtggc ggcccgacgg gatcgcaacc gagctgcatc cgcggcgccg
     1561 caacgccgag gaggacgagg cgtatcagcg cagtcccatc catcacctgc gcacatccgg
     1621 agatgccgcg ctgcgccggc gtctcgacgc cagccatgcc gacgactttc cgatcctcgt
     1681 cgatctgcgc gaggaaggca tgacagatta cgccgcgacg atcgtgcgtt tcagcgaggc
     1741 gtcgaatggc gacgcgcgtg ccgaaagggt gcgagctgcg ttcgatcgtc ccgacccatt
     1801 gcaggggata ttcttttcct gcgccaccga cgacccgggg ggcttcaacg acgagcatct
     1861 gcgccaggtg accgagcttt tgccctatct cgccctcggc gtgaaatcgc gctcgacgtt
     1921 cgatgtcgca caaacgctgc ttgaaaccta cctcggcccc gacgcggggt cgcgcgtgct
     1981 gacgggtgaa atcgaccgca actcggtgcg caacatccag gccgtcatct ggctctgcga
     2041 tctgcgcggg ttctcgcgaa ccgcaagccg cgtgacgcag gacgagctca tcgagctgct
     2101 cgatgtctat ctcgaagtga tggcgcggcc ggtgctgaac aaccgcggcc agatcctcaa
     2161 gttcatgggc gacgggttcc tcgccacgtt cgatcttgca aaacgcagcc gggaagccgt
     2221 gtgcgccgat gcggtgaacg cggcacagca gctgctcggc acgttgcccg cgctgaattc
     2281 ggagcgtcgc gcgagcagca agacgacgct cgatcttggc gttgcaattc acatcggcga
     2341 cgtgatgtac ggcaacatcg gaaccaggga gcggctcgac ttcaccgtcg tcggctccgc
     2401 ggtgaacgag gtgagccgca tggagggact gtgccggccg ctcggacgca acgtgctcgt
     2461 gtcgcgtacg gttgccgagg ccgcggcggc gtcgcgggga aagttgctct cacttggcgt
     2521 ccatgcgctg cgcggcatcc gcgagccgca ggagttgttc acgatcggcg acgcgtgatc
     2581 cgggggttgg gccaccgcgc aaccggcgga tcggcttcgc cgaatcgcca cgcggcgtac
     2641 gtgtgcaccg gggtcgcacg ccggggatgg gttcgacgtc gccgaaacac gacgattcct
     2701 tgacacgcgc gcgcaccagc tggtattcgc gtacgggcag gttcacaggg agacgtcgaa
     2761 ttgacaagaa caaatgcaac cgggacggcg ttcgcgcttt ttttgctcgc cctgttcctc
     2821 gccataccgg gtagttccgt tcgcgcgcaa actgcgcggg gctgccttgg tgacgtcgtg
     2881 ccgcagcacg ggccgcttcg caccgaagac gcggccgaca cgcgcgcggt gctgcctttg
     2941 cagcaggacg gcgccgatct caccgtgacg actccgtgca tcgtgaaggc cgggacgtac
     3001 aagttccggg acgttcacat cctcgatcag gggcgcttga tattcaccga tgcgcggatc
     3061 gacttctggg cggccaacat tctcgtcgag gacggcggca gcctcgttgc aggctcgccc
     3121 caggcgccga tcggcaccga cggcggcgtc gtgacgatcc acctgtgggg tgccgaccag
     3181 accggcgtcg atccgaaaaa gcagggtatc ggcatcgcgt gcatcggcga tagcgtcggg
     3241 cggtgcggcg tgcccgacga gatctggcaa tccaacgtag acgcaatggg catgccggta
     3301 ccggtaagca aggcgcgcaa gatcgcgtcc cttgccgcgg caaggcagta cccgggcgtc
     3361 accgacgatt acttctacgc gtaccagccg ctcactttcg acggcgggtc ggacgcgacg
     3421 cacccggtcg gctatttcgg ctccaaggtg atcggcgttt cctacggcgg cacgttgcag
     3481 ctcttcggca ggaagggcgc aacgtacgca acgcccgagt gcggcgacgc acccacgacc
     3541 agcgggacga gttgggcgcg gctcgatcgc cctgccccga agggcacgag cctgctgacc
     3601 gtggaccgcc cgatgtcgct gcaagcgggc gaccggatcg tcgtcaccac caccgactat
     3661 cttcccgggc actccgagca attgacggtc gccaccgacg tggcctgcgg aacggccatc
     3721 gcggtcaggg aagccctcgc ctacgcacat tacggcgcgc gctttccgct gaccgtcgta
     3781 ccggaggacg tcggtctcga tccgcagctg cgtgccgcgg gcgcggaaac gcgtgcagcc
     3841 gtcggcgtgc tgacgcgaag catccgcatc gtctcggcgg gcgacacgat ggacgacgac
     3901 ttcccgcccg agccacccgc cgggtcgacg acaccgggct actacttcgg cggccacctg
     3961 atcgtgcgcc aggggttcaa gacgtttcag ctgcagggag tcgagctcca ccagatgggc
     4021 cagggtggca ggatcggcca ctatcccgtg cacttccatc atgcgcggcg cacgcccgcg
     4081 aacacgttcg tgcgcgactc gtcgatcgac gactcgatga cgcgctggat cgtgctgcat
     4141 ggcacgcagg acgtgaagct cgagcgcaac gtcggttaca ggtcgatcgg gcacggcttc
     4201 tacctcgaag acggcaccga gatcaacaac gtgcttaccg cgaatctcgg catcttcgcg
     4261 cgcgccgcgg tcgacaacgt gcagaacccg cgcaaggtgc cgggcatcct cgccgcaccc
     4321 gatctgcacg ccaccaccgg cgaggccgtt ccttaccggt cggattacga ccatccgacg
     4381 atcttctgga tcatgaacgg ctggaacgac ttccgcgaca acatggcggt cggcgcgggc
     4441 acctgcggtg tttgctattg gcttctgcct gccatcaaca gcacgatgtc gcgcgacatg
     4501 aaatggtcgt cgtacgcgtc gatgcaaagc acgctcgatc gcgccggcat ggcgccgctg
     4561 aagtcgttcg tcggcaacgg ctgctcggcg gcgatgacat cgttccagac gatcaccgcg
     4621 acggaggcat gccagggcgt gggcccgggt gacgccgcct cgccgcatcc gctgaacctg
     4681 ttgccggtca aaaatccgct cgcgacgccg agcaagccga aggaggcggc cgccgactac
     4741 tatccgagcc tcggtgacgg cgggcacttc cccacgaagt gcgcgcagga agattgcagc
     4801 agtcgcccgg tgcgctgcag cgcaggcaac gcggccgact gcatggtcac gatcctcgat
     4861 cactacacga cgtcgttcaa ttgggcggcg ttcaacttcg cggcgatctg gctgcgtccg
     4921 cagtggtatc tcgtcaccga cagcgtcatc accgattcgc agcaggcggg gctcacgatc
     4981 gtcaccggcg gcggctacag cgattcggac gtcattccgg gccattgggc gctggtgcgc
     5041 aagagcgtgt tcatcggcaa tacgcaggac gacaatccgt ttgcgtcgaa cggcggcccg
     5101 ttcaatcccg gcgcgctgcg atgcgcgatg gacgcgaacg gctcccggcc gggcaatttc
     5161 tgcctgtcca tcgacgaggg cgtatcgcac caggtcagca acttcggcat gtaccagcgc
     5221 ctgttcagcg tgtacgacgg tcccgcgttc caggactcga atgcgtacct gcacatcaag
     5281 acgcgcagga tcgacgactg ccagccattc gtcgacaccg tgaacaaggc cggtcgctgc
     5341 gatcccgtcg atttgagccc gggaaagccg cggcaaagcg cctggctcgc cggattcgtc
     5401 gccgggctgc cgaaggccgt gggcgccggc ggcccgttct gctacatgcc caacgccgcc
     5461 atcggctgga agcagcccaa cgggttctac tacccgcctg cattccattc ggccaatctg
     5521 tttttcaacg acgtcgacat ccggcatttc gtcgtgtcgc cgctgttcgt cgagggcacg
     5581 tacgacaccg acatcgccag ggttgccgac aagtactgca tctggaacag ccagctcttc
     5641 accggcttcg ccggcaacga ccgccagacg gtgctcaatg acgacgacgg cacgctgacc
     5701 ggctaccgga agacgacgat catcaaccag gacgcgttct tcgccgcccc cgtcgacgcc
     5761 acgcagtgcg attccgatga cacgtcgcgc acgagtccct atgaatacgt gacgaccgtc
     5821 ctctatccgg cgtgcgtgct gaacggaacg tgcgctcggc cgcccgacgt cgcgccgaag
     5881 ccgccggatc cgcattggaa cgacggcgac tggaaccgct cgtgcacgag cgagaaatgc
     5941 tacggcattc cattgtggcg gcaggacctg atgcccgtcg cggacaaggg tgccgccaag
     6001 agcatccgca tgatgggaca ggagacggcg cagcgcagct cgctcaccgt caaccacggc
     6061 acctactact tcgatacttc cgtttcgaag gagacgcagc tcatgcaggg ctgcacgacc
     6121 accgacccgg cgaatccctg cgtgatcaac gtcttcaaac ccaaccagac ctactacctg
     6181 tttctcatct tcgccaaggc gaccaccgag cagacgtatc gcttctacgt gggagacgac
     6241 accgacttcg acccggcgag catcgagctc gtgcaggcgc agatcggccg caatccggtg
     6301 aagtacgaga cggtcggtcc gctgccggcc gggcgggcac actggctcga caacgaccgc
     6361 gcgaaagccc acggcgtcgt ggaggtcgat ctcatgcttt ccgatcttcc cgatgtggca
     6421 acgttgttca cggcggcgaa gaagaacaag tgccagccgt cgacctactg caagtggaac
     6481 gatgcgaccg aacaatgcgt cgacgcggac ggcagcgacg ccgtttgcaa atgggcgatt
     6541 gcagaccagg actgcccggc gggcggctgc ttcggcattc gattcacgct gccgagcggc
     6601 ttcgcaacgc gcgcggcgaa cgacgcgcgg cccgatccac ggccggttgc gctgtgcgtt
     6661 ccgaaggatg caccgtggga cgtgtcgctc gacgcgctga aggtatccga tggcgtgtgt
     6721 ccgaaggagc cggacaggca gccgctggat tactgcgcgc agtgagtttc gctcgcgcga
     6781 tggcgcaagc ctttgcatgc ggcccgcaaa ccgttcatct cgacgcgagt gtcacccgga
     6841 caagtgtgcg tgaatcagtc cgacggctgc ggcgccttcc ccgatcgcgc cgcccacccg
     6901 cttgaccgac cccgcgcgca cgtcgcccac ggcgaacacg ccgggcacgc tggactcgag
     6961 cgaagcgggt gcgcgccggc cgctcgcgcg tgacgcggct tcaccggtca gcacgaaacc
     7021 gtgctcgtcg agcgcgacgc cgcagcttcg cagccattcg gtctccggat cggcaccgat
     7081 gaacaggaac acgtttcgca ccggatgtcg ttgctctgcg ccgctgcggt tgtcgcggca
     7141 ggtaatggcg gcgagcctcg cgcccgcatc ggcgtggagg tgcgaaagtt ccgcgttgta
     7201 atgcacgtcg atcgcggggg tggcagcgat ccgatcgatc agatagcgcg acatggtcgc
     7261 cgcaaggctt tccctgcgca cgagcagatg gacccgggcg gcgtgcctgg cgaggtatac
     7321 ggccgcctgc ccggccgaat tgccgccgcc gacaatggcc acttcttcac cggcgcacat
     7381 tttcgcttcg agcgccgagg cccagtacca cacgccgcgg ccttcgaact cggcgagccc
     7441 gggcaccgcg ggacgtcgat agcgggcacc gctcgcaatc accacggtgc gcgaacgcag
     7501 cgtgcgctcg tcgccgaggc ggatccggaa ttcgccgtcg tgcgcggcat cgtggcaatc
     7561 gagcgacatc gcctgcgccg gaatgagaat ctcggcgccg aacttttgcg cctgaacgaa
     7621 ggcacggcca gcgagcgcct gtccggaaat gccggtcgga aagcccagat agttttcgat
     7681 gcgcgcgctc gcgccggcct gacctccgaa cgcgcggcac tccacgacga tcacgtgcag
     7741 tccttcggac gcggcataca cggcagtggc gagcccgccc ggtcccgccc cgacaacggt
     7801 gacgtcgaag cgctcgtcgt gcacggcgga gtcgagcatg ccgaggcagc ggccaagcgc
     7861 atcctccgag ggcgtgagca gcacgccgcc gttcgggcac accaccagca cgtcgccgct
     7921 cgcgccaccg tactgcgcga aaagcgccgc ggcggccggt tcctgcgacg gatcgatggc
     7981 gtggtgcggt tgaccgttgc gacgcaggaa attctccagt cgcagcaggt ctggcaactg
     8041 cggcgtaccg atcaatgccg gaccggcctc ctgttcgatc aatccgacgc gccgcaagat
     8101 cagtgcgcgt gtgatgcgct cgcccagctc cgcatcggcg acgatcaagg cgcgaaggcg
     8161 ctcagtcgag atcagcagtg cttcgacctc ttcgtcggca acggcatcga cgaccgcggc
     8221 gccgccggag agctgtgcga gctccgccag aaactgtccc ggcccctgcc gcacgatcgg
     8281 cgcgacatgg ccgagtccat cgcgctggct gatcgtcacc gtgcctttca gcacgatgag
     8341 catgcccggt atggtttcgc ccgccgtata gaggcgtgct ccggccggaa agcgccggag
     8401 cgcaccgaat cgacgcatgc gtgcgatttc ggcgtcggtg agcgcgggaa acatctgcgc
     8461 aagccggctc gcgatgcccg cggatggccc gccgggaatc agccattcgg agtttggcgc
     8521 ttgcatgcgg gtgtcagcgt tcgatcggga gcacgccggt cgaggcacgt aatgcgctca
     8581 ccggagagcg cattctggaa ggcttgatcc actttcgcaa cgagtgcggt acgctggcgg
     8641 ttgcaaacgt gacgatggcc acaacatcca ggacaatgac gtccgctgcc gcgttccgct
     8701 tcgacaattc cttcgcgcgc gagctcgaag ggttctatgt tgcccggcga cccgccgtcg
     8761 tgccggcgcc aaggttgctt tatctgaatg agccgctggc ggatgagctc ggcctcggcg
     8821 tcagcgcgct cgacggcgag caaagcgcag cgatattcgc cgggaacacg ctgccggacg
     8881 gtgcgcaacc gctggcccag gcttacgccg gccaccagtt cggcggcttt tcaccccaac
     8941 tcggcgacgg tcgcgcgctg cttctcggcg aggtcatcga ccggcacgga aagcgtcgcg
     9001 acatcgcgtt caaagggtcc ggccgcacgc cgttcgcacg cggcggcgac ggcaaggcgg
     9061 cggtcgggcc gatgcttcgc gaagtgctgg tcagcgaggc catgcacgcg ctcggtattc
     9121 cgacgacgcg cgcgcttgcc gtcgtcgcga ccggcgagcc ggtgtaccgc gagcaggttc
     9181 tgcccggcgc ggttctcacc cgcgtcgccg caagtcacat ccgtgtcgga acgtttgagt
     9241 tccatgccgc gcgcggcgac atggagagcg tgcgcaagct cgccgactac acgatcgcgc
     9301 gacacgatcc ggggctcgct gcgtcgccgc aacgcttcct tgcattgtta cagcacgtgg
     9361 cggagcgcca ggcggcgctg atcgcgcagt ggatgaacgt gggcttcatt catggcgtga
     9421 tgaacaccga caacatgacg atctcgggtg agacgatcga ttacggcccg tgcgcattca
     9481 tcgaggccta cgatccggcc gccgtgttca gctccatcga cgagcaagga cgctacgcct
     9541 tcggcaacca gcccgacatc gcgtgctgga acctcgcacg cctggccgag acgctgttgc
     9601 ccctcctctc cgaagatcaa caggaagcca tcgcgatggc caacgaggtc atcgcccggt
     9661 tcccggcaca gtatcgccgg cacctgcttt gtggacagcg cgcgaagctc ggcttgcgac
     9721 gagccgaacc ggccgatgac acggccgaca cggcgctggc cgacgactgg ttgagcctgc
     9781 ttcacgccgg gcgcgcggat ttcacgctcg catggcggcg cctcgccgac gccgccggcg
     9841 gcaacgaagc gccgctgcgt gcactgttcg ccgacgcgcc tcgcctcgat gcatggctcg
     9901 cccgctgggg ggcacgctgc gcgagcgaag ccggtggcga aacgccgggc acaacactcg
     9961 agcgcgccga tgcgatgcgc cgggtcaacc cgatcgtcat tgctcgcaat catcgcgtcg
    10021 aggaagcgct cgctgccgcg tccgaccgcg gcgatctcgc gccgttccgg cggttgctcg
    10081 atgccgtcag ccacccgtac gtcgagaccg tcgagttcgc ggcctacgcc gagccggcgc
    10141 cggccgcagt gaccgcgtgc taccggactt tctgcggcac ctgatcggct cacggcgccg
    10201 ggccgcacgt cccggattgc cgggtcttca tgcaacgggc atgcgttctt atcgcgtgca
    10261 gttctgcctc cttgagactg cgttcggccg agccgatcag cgcgtcgcga tgcgcgccgc
    10321 gatctgccgt caattcccgc gccggtaact gaagcggccg cggcggctcg cacggagcgg
    10381 gaagaccttc gccttccagg catcgctcat atccgtcgag cgcatgaatc tgtgcgttca
    10441 gcatcttgat gagaagcgaa tcctccgggg cctgagttgc gccttcggta tgcgctgcat
    10501 acgcggcgat gggaaaggcc gcggccagcg caatgatcgc caggcattgc gtccggacgc
    10561 cgcagcgcgc ggcaagcggg gcggacggcg gccgcaacat gtggaacgag agcatcgcat
    10621 ttcctttacg catggtgatg aaatgccgcg tgcggtcgcg cgcttgcatt gcattcggga
    10681 atttcgcgcg gacaaccgca acgcgcggct gcggaggcta ggcgatgccg ggagggccgc
    10741 aactcggcgg cagcacgacg acaccggcgc tctgcgcgag tgacggtggg ccaggatccg
    10801 cgagccggaa cccgttaacg gcgagcggga ccggagcgac gaggttcggc gccggcccct
    10861 cgagcttgcc gatgccgacc gcagcgcaga tcgcgcagcc ctcatccagg tcggcgacaa
    10921 cgtgcaggtg actcgcggcg gccgatacga gaatggcgaa tgcgagaacc gtaacggccc
    10981 agaccagccg gtcgtgacga cgatccatgt acgataatga taattcattc gcatcatgca
    11041 caacgcttga tcccgaccct gatggtgaaa gtccggaagg aacacacgca atgaattcga
    11101 tcggcaagac gagatgcgtt cgcttcgtat tcatcgcctg tgtcgcatcg ctcatgggcg
    11161 cggccgcggc cgcggatgtt catcgcgcgc cggcgccacc ggccaaggcg ccggtgcgcg
    11221 acgtcaccga cacccatttc ggtgtcaccg tcgacgatcc gtatcgctat ctcgaggacc
    11281 tcaaggatcc cgacgttgcg gcgtggatca agtcccaggc gcattacacg cgtgaggtgc
    11341 tggaccgcgt gccgcaccgg caggcgctct atgaggagat cgcgaagtac ggcgattccg
    11401 catcggcgcg cgtgacgcac gttcagttga gtggaagcta cgtctattac gagaagcgcc
    11461 gagccgccga caatctgccc aagctctatg tccggcgggg cttgcgcggc aaggagcggt
    11521 tgctcgtcga tccggagcgc ctgaaggcac ccgacggcgc gcattacgcg atcgattact
    11581 acgcgccctc gccggacaac cgatacgtcg cgtacggcat ctcgctcggc ggatcggagc
    11641 aaagcgtgct gcacgtgctc gaggtcgcga caggccgcga aacgggcgac gtgatcgacc
    11701 gggccaacta cgcatcgccg tcctggctgc cggatggccg gttgctgtac agccggttgc
    11761 agaagcttgc gccgggtgcg cctgtcaccg acaagtatca gaaccagcgc gtctatctgc
    11821 atcgtctcgg aagtgacccg gagcaggatg ttgccgtgtt cggtgcgggc gttgccgcgc
    11881 agatcgacgt gcggccggtg gagctcgtat tcgcgggcgt cgcgccaggc tccgattacg
    11941 tcgtggcact cgcggtgaac ggcacgcaac gcgaggtgcg catctggacg gcgcgccttt
    12001 cgacgctcga tggcgcgaaa acccgctgga cgggcgtcgc cgacacgggc gaccaggtca
    12061 cagacgcgag cgttgcagga aacacgctgt tcatggtgtc gcacaaggac gcgccgcgct
    12121 tcaaggtgct gcgcttgccc ttgtccgcgc ccgatatcgc gaaggccgat gtcgtcgtcc
    12181 cgcctgccga gtcgatcgta acgggcatcg cggccgcgtc cgatgcgctt tacgtgcggc
    12241 ggatgaatgg cggcatcagc gagttgttgc ggttgggttt cgacgcggcg ggaaccgcga
    12301 aagcgatcaa gcttccttac gacggcgata tcgatgcgct ggccgcggat ccccgcatgc
    12361 cggggctgct gttcaccatg ggcggatgga cgcgcttcgg cagcgtgttc gcgtacgctc
    12421 ccaagtcagg tcggatcgcc gataccggcc tgcagccgca gggtccgtac gacaatccgc
    12481 gcgacctcgt ctcggaggag gtcaaggcga cggccgccga cggaaccgaa attccgttgt
    12541 ccatcgtcta caggaagggg ctccagcacg acggcggcaa tccgacgatc gtatggggat
    12601 acggggccta cggcatttca cagacgccgg tgtatgcgcc cacgctcctt ccatggtacg
    12661 agcgcggcgg catattcgcg gtctgccatg tgcgcggtgg cggcgagtac ggcgaagact
    12721 ggtacaaggc cggcttccag gcgacgaagc cgaacacctg gcgcgacgcg atcgcctgcg
    12781 cgcagtggct cgtcgaccat cgctattcgt cccctgccaa attgagcatc cgcggcggaa
    12841 gcgccggcgg catcctggtc ggccgagcca tcaccgagcg tcccgatctg ttcgccgcgg
    12901 cgatcgacga cgtaccggta tccgacacgc tgcgcatgga gttctcggcc aacggcgttc
    12961 ccaacattcc cgagttcgga tcggtgaaga ccgaggcagg attcagggcg ctgctcgaaa
    13021 tgagcccgta cgcgcatgtc aaggatggcg tcgcttaccc ggccgtgttg ctcacgacgg
    13081 gtttcaacga cccgcgggtc gacgcgtggg aggcggcgaa gatggcggcg cggctgcagg
    13141 cggccacgag cagcggcaaa ccgatcctgc tgcgcgtcga ctacgacgcc ggtcacggct
    13201 tcggcacgtc gaagaaatct gcttaccagc ttgccgccga tcgctttgca ttcctgctct
    13261 ggcaactcgg cgtcgcgggt tatcaaccgc cctcgtgatg cgacgagacg tcgacgacct
    13321 tgcatcgaac gcggcggagt cctgccagcg gatcggacgt tgacgtgcat caatgcagcg
    13381 cggcgtgatt cggcgacaat cggcctgcgt tcgacccgga agcgtgcgca acacaccgat
    13441 acatcgcgtg ccgcggcgcg acccggacgg tggagacgaa ggatggacga agagatcaga
    13501 cggaaaatcc agtcgcttct ggatcagcac cgggcgatga cgatcgcaac gatgaggccg
    13561 gacggctggc cgcaggcgac cacggtgggc tatgccaacg agggcctgac gctttacttc
    13621 ctttgcggcc cggaaagcca aaaggcggcg aacctcgcac gcgatgatcg cgtgtcgctc
    13681 accatcgatg acgatactcc gcaagtgatg gagatcagcg ggctttcgat ggctgcacat
    13741 gcgcaagtcg tcgacgatcc ggccgaagcg gagaaagcgt tgcgtttgct gatgctgagg
    13801 tatccggagc agaaatccat gccgtttccg atgccgacgt ccgcggatgt gcgcatcttc
    13861 cggctcgtcc cgatggtcgt ctccgtcctc gattattcca aggggttcgg acataccgac
    13921 ctcgtcagct gccagtaggc gcggtacgca tgacgacgac acgatgacga ccgaaccgcg
    13981 cgtccgattt tcgaatatcc agacggggct caccggtgac gcgccgttgc accgcggaaa
    14041 gtgcgcgagc gtgctcgcga gcttcgcgcg cgatggcacg ctacatcgtg tcgcttcagg
    14101 tacagacgaa ggcgtcgagg caaccctgat cgacatgcga tgcgcaagcg gtacacggcg
    14161 ccgcccgaga tgaactgaaa gcgacacccg ctgcagatga gccgcgttcg cctgctgctc
    14221 gatgctgttc gcgcccatgc gcgggcgatc ccgggtcgcg acgatcgtcg cgccgggaag
    14281 cggctgcgcg ctctggttca tggtgctcga caagtccgtc ggcgtcgata cggagaagcg
    14341 caccgttgcg caagctcgcg cggcgcacgc tgatggacgc cgcggcgtgg cgcgcgcagg
    14401 cgcgatgaat cgccctcccg ggctcgacag cgaagctgcg cgagcccgcc tcgcgcggca
    14461 agggccgaac gagttgccgc gaagcgcccc acgctcgttc atcgcaaata cggcgcatgc
    14521 gctggcggag ccgatgttcg gtttgctcgt ggtcgccgcg tcgatctact ttgcgctcgg
    14581 cgatcgcgtc gaggcgctgt tcctggcggc gtccgtggtc gtcatgatcg cgatcacgct
    14641 ctatcaggag caaaagaccg cgcgcgtgct gcgcgcgctg cgcgacctcg cgagccctcg
    14701 cgcgctcgtg atccgcgacg gcgtcgggcg tcgcgtcccc ggacgcgaag tcgtcgtcga
    14761 cgacgtgctc gtcgtacgcg aaggcgatcg cgtcgcggcg gacggtcgcc ttatcgaagc
    14821 cgacgatctg cacgtcgacg aatcgctgct gaccggcgag tcggcacccg tcgccaagaa
    14881 agcgtcttca tcgtcgccac cggcctcgct cgagtcgaat gcctgcaacg tctatggcag
    14941 cacgcttgtg gtccgtggac agggcatcgc gcgtgtgacc gcgacgggtc cggaaagcga
    15001 aatcggccgc atcggccgat cgctggaacc gttggcgacg gaagcgaccg tgcttcaggt
    15061 cgagatgcgt cggctggtgc tcgtcttttc cgtcgtcgcg cttctggtgt gcgccgtgct
    15121 cgtcggcgtc tacgtcctga cccgaggtgg ctggctaaac ggcgtgctag cgggcttgac
    15181 cctcgcgatg gcgatactgc ccgaggagtt tcccgtcgtg ctgacgttat tcctcgcgct
    15241 cggcgcatgg cgcatggcgc aggtgcaggt tctcacccgg cgcatcgcgg ccatcgaggc
    15301 actgggcgcc gccacggtgc tgtgcgtgga caagaccggc acgctgacgc aaaaccgcat
    15361 ggcggtgcag cgcatctaca gcgcgggcca acgcatcgac gtcgcgtccg ccagcgcagg
    15421 tgcgcttccg ccattgtttg ccgaggcggt tcatgtgagc gttctcgcga gcgaaccgca
    15481 tcctttcgac ccgatggagc gcgcgcttct cgacgtcgat ccggtcacgg acgcaccggc
    15541 gctggaactt gtgcgccgct atccgttaac taccgggcgc ctcgcggtga cgcacgtttg
    15601 gcgcgatcgc gatgcaaccc acgtgatggt ggcggcgaag ggtgcgccgg aatgcatcgt
    15661 cgaactgtgc gggctgacgg cagaccacgc cgccggggta ctgatcgaag cggcggcgat
    15721 ggcggcgtcg ggattgcgcg tcctcggcgt tgcgaaggcg gagcacgatg cgaccagcct
    15781 cccggaggac ccgaaagacc tgcacctcga cttcctcgcc ctgatcggtc tcgccgaccc
    15841 gctccgtcct gccgtgccgc aagccatgcg cgaatgtcgc gccgccggcg ttcgcgtgat
    15901 catgatcacg ggtgactttc cagtcacggc cgtcgcgatc gcacgcgaag cgggactcgc
    15961 cgagcagccg gcagtgatga ccggccgcga gctcgacacc atgagcgacg acgcattgtg
    16021 cgcgcgcctg tccacggtcg acgtcttcgc gcgcgtcgtt ccctcgcaga agcttcgtat
    16081 cgtcaattca ctgaagatgc gcggggacat cgtcgcgatg accggcgacg gcgtgaacga
    16141 tgctccggcg ctgaaggcgg cgcatatcgg catcgcgatg ggacgccgcg ggacggacgt
    16201 ggcgcgcgaa gcggcggcgc tcgtgctgct cgacgacgat ttcacttcga tcgttcgtgc
    16261 catccggcag ggacgtcgca tcttcgacaa tcttcgcaag gccatgtcgt acctcgtctc
    16321 ggtccatgtg ccgatcgccg gtctcgggct cgtgccggtc cttctcggcg gtcccctcgt
    16381 cctgttcccc gcgcatatcg tctttctgga attcgtgatc gaccccgtgt gctcgatcgc
    16441 attcgaagcg gagccggcgg aaccggacgt gatgcgtcga ccgccgcgta gcgcccgtcg
    16501 ccgcttgctt ggcggcctgc ccttcgcgct cgccgtgctc gaaggcggcg tcgcgcttgc
    16561 gttcaccgtc gctgtctacg cgatatcgtt ggcgagcgaa ggcggcgaga caaggaccag
    16621 gcttctctcc ttcacggcgg tcatcatcgc gaatctttcg ctgatcttct ttgcgcgggc
    16681 cggaggccgg cgaatccgcc ggcacgtcgc cgcggcgaac cgctcgcttt ggctgatcgt
    16741 ggccggcaca ttggccgcct acggcgtggt cgttgcggtt gcaccgtttc gcgaagtctt
    16801 tcgaatggct gcgccgactg cagccgatgc gcgccttctc gtcgcgacca cgttgctgct
    16861 gtgggcgctg ctcgcgacgc tgaacctcgc ttacgggacg ttggcgaagc gttcggggca
    16921 gcacgcggca tgatccgttg acgttggtca atacgcgaca ggcgcatggc gggttcaatg
    16981 gatggcgcgg gaacgcgata ccccgcgcca cccataccag cggaggtctc catgttcaaa
    17041 cacattctcg tggccctcga cggcagcgcc gcgtcgaatg ccggcttcaa atcggcactg
    17101 cagcttgcaa gcgatcagca tgcgagcgta accggggtga gcgtcatcga tgatgcagcg
    17161 gtcagggcga acttcgacgg cgcctacctc gccgcgagct acgtcgatac gctgtacgac
    17221 tcgctatgcg agaacgggcg cgcgatcctc gccaaggcgg aatcggccgc acgcagcgcc
    17281 ggcgtcgcat tcgagccgct tctcgtcgaa tcgcgcggac agacggtggc gcacgccata
    17341 ctgcagcagg cgcgaaaggc gaaggtcgac gtgatcgtgc tcggcacgca cggccgccgt
    17401 ggcttgtcgc gcatgctgat ggggagcgat gccgaagctg tcgtacgcga gtcgcgggtt
    17461 cccgtgctcc tcgtgcgaag tggggaacgt gccacccgcc ggcgcagcac agcggcgatg
    17521 cctgcggcgg cgtcatcgcg caagcgtggc accaccgcgc agcccaagcc acgcccgtcg
    17581 cgcacgcagg cataggatgc cggcgcgttc gccgcgccgc cttctctcgc tcctgatccg
    17641 ccacgcatgg caccgagaca acttaccttg tcgctgtcag cgcggcgcgg attgcgtcga
    17701 cggagcgaag cgccaacgcg gcgatcgtga gcgtcggatt ggcggctccg cctgtcggaa
    17761 acacggcgct tccttcgatg aacaggttcg ggtgatcgaa ggcgcgcagg ttgccgtcga
    17821 cgaccgcacg ctttggatcg gtccccatgc gtgccgtccc catgacatgc cctgccccct
    17881 ggaaatcctg atcggcgttg tgcgcgatct cggtcgcatt caagcgcttg aatacctgct
    17941 cgtggatccg acggccctcg gcgagacccg ccttcgtgta gtcgtcgatc cgataggcga
    18001 tgcgcgggcg cggcagcccg agcgcgtcgt gctcgatcgg atccaaagct atgcgattct
    18061 gcggatcggg tagctgctcg gtcagcgtcg cgaaacggaa ttgccgcgcg gtgcgctcat
    18121 acagcgcttt ctgcagtgcc gcgccgcgca gccccttccc ggcaagcgca cgcgcgtcgc
    18181 tcgccggcgc cccggtcggc cacgaccagc cgtcgttccc gatctcgatg cgaaacgcgg
    18241 gacgcattgc gcgccagtcg ccggcccgcg tattttcgat gcccgacgtc gacaacggtc
    18301 cgcgatacgg atagacgggt gattcgctca gcgcccagga caactggatc gggtggtcca
    18361 tcaggttccg gcccacttgg tcgctcgaat tggcaacccc gttcggggca ttcggcccgc
    18421 gcgacatcaa aagcagcttg ggaatctcga ccgcgtgtgc cgcaaggacg tacaccttcg
    18481 ccgtcgcgcg atggtcgctc ccgtcgggcc gcttgtatcg gaccgcggcg acgttgccgt
    18541 cgccgccgat ctcgatgcga tgcacgacgc accgtgcgag aagccgcgcc ccgcccttct
    18601 ccgcccgcgc tacgtgcacc gtggcgtcgt acttcgcctg gatcggacaa accgggatgc
    18661 agctgctgtt gccgcagcac ggcggacggt cgtcgcgcac gacagaattg cggccttgcg
    18721 gcgtcgagcg cacgttgaga cccgattcct cgaacgcctt ggtgatcgcc gcatccaggt
    18781 agctcggtgc gatgcgcggc atcggatagg gcgagcttcg cggcgatccc agatccgcgc
    18841 tcgaatcgcc ggcgacgccg attgcctcct ctgcgcggcc ataccacggc tcgagcgccg
    18901 aatagtcgat cggccagtcg accccccgac cgaagcgcgt cgcaagccgg aaatcgtcgg
    18961 gcaccaggcg caggcacgtg ccgagccaat gccatgtggt gccaccaacc tggcgcaggt
    19021 aagtgctttt gaacttgtcg cggcccgtct gcacgtaata gaaatcgaga tcgtcggaac
    19081 gtggatgcgg cgcatacgca gtgttcggat aggcgcactc gggcactttg atcgcggcgt
    19141 tgaagaactg tgcgaccgcc tgctcgcgct ggatgcgcgg ccccgcttcc aggatcagca
    19201 ccttgacgcc cgtcgccgca aggcgatcgg cgaccagcgc gccggatatc ccggcgccga
    19261 tgacgatgac gtcggcgtcc atcgtcaggt cggcggatcg gcccagtagt tcgtcgcgcc
    19321 gccgcactgg cccatcggct tggtgaacga cattgcggac cacatgtacg cgccggcgta
    19381 gagaacgacc cgtggcgtcg cgccttgcgt gacaacaccc gaataccacg ctgcgacgag
    19441 ctcgtcggca atgtcgtcga gaccctgcac gcgcagcgcg gcgtcgagat ccgcggagcc
    19501 cgctgcgtcg acgacgcgag cgaggcgcgt cagcgccgcg cgccgcgcgg gtgtgccgaa
    19561 cgccgcccac agtttcgtcg cgtccgccgc cgaaggcgcc gggtagccgg tcagcgcggc
    19621 ggagacgacg ccaaaattgg cgacggacaa cggagcggcg gccggtgtcc cggcccccaa
    19681 cgccgccagc gtcgcgccga cgatcgactg gagcactcga cgacgatcga cgtccatccc
    19741 gtcctcccgg ttagaagtgg ggaccgtaag cgatccctcg cgtcagcgca acccttccgc
    19801 gacccgcgcc ccgacgatgc gccgacaatg ctcgcgcgcg gcggcgacca cgttgcggct
    19861 cgcttcgggc gtgcggaatg cggaatgtgc cgagagcgtg acgttcggca ccgtcgtcag
    19921 cggatgaccc gccggtagcg gctcgacgtc gtacacgtcg aggcctgcat gcctcaaacg
    19981 accgtcgcgc aatgcatcga tcatcgccgc ctcgtcgacg agcgcgccgc gcgccgtatt
    20041 gacgaggatc gccccgggcc gcatcgcggc gatccgtgca cgggagagaa atccgcgcgt
    20101 ctcgtcgttc agcagaagat gcatcgatac gacatcgctt tcggcgagca ggcgttcgag
    20161 cggaacgaac gtgacgccgg catgcgcctt cggactgcgg ttccacgcga taacgcgcat
    20221 gccgcctccg agcgcgattc gcgcgacctc tgctgcgatc gagccgaagc ccacgagccc
    20281 aagcgttcgg ccggtgagct cgatgccctc ggtgcgaagc cacgttcccc cgcgcatctc
    20341 gcgatccatt cgggcgacgc cacgtgccgc tgcccacatc agcgcgatcg tgcactccgc
    20401 caccgccgta tcgccgtaac ccttgatcgt gtgtacctgg atgccgagcg cggcgagctc
    20461 ctcgacgctc atgtagctgc gcgcgccggt gccgagaaag atgacgtcgc gcaagccgac
    20521 gcattgcttc gcgatcgcgg taggcagagg cgtgtgatcg atgatggcga cgtcggcacc
    20581 cgcgagcagc ccgggcaacg attccgggcg cacttcggga tcacgattga cgctgaatgc
    20641 gatgccgtcg acgcgaaaca gctcgtcggc gagggacgcg agcgccggcg tggcatcgac
    20701 gaagacggca ttcatattgc gacgcaaggg cactttccaa ccttcgctcc cgccacgtgc
    20761 ttcgcgacgg atatgcttac ccaatcacgg tacgcaatcg tcattgcgga agcgtgtaac
    20821 gggtctcgat cgctgcgcag acgagcgccc gtgtctgcgc agggggcaga tcgatgcgtc
    20881 gctcgatgtc ctcgaccgct gcggcgagat cgcggttctt gatcacgtcg cgcatccagc
    20941 gcgagtagtc gttgcggcgc aggtggaaca gccacgtctc ctcgccgatg ccttcggcga
    21001 tatggcagaa cacgctcagg ttctgcgcct tgaggttgtg cttgccggtg gggccgcgaa
    21061 aatagaagct ctggtagcgg acgtcgccct cggcgtactt ccgatggtga cggatgcgct
    21121 ccgcccgccc cggtatcgcc gacatcgcaa aggggccctc ggcggcatgc acgaaccagc
    21181 aggcgacctg atcgttgtcg acatgcaaag cgtgcaccgc gtccgcggcg cggttgctcg
    21241 ctgccgcaaa ctcgcgcaac gtcctgtcgg gcgatgcccc caccgcaatc acgacgtcga
    21301 cccacgcgag gatcgacgga gcgagacgct ccggatgtac cgtcaccagg atgctttcgt
    21361 tgaagcgttc cgtcggatgg ccccacgacc tgggcagcag gtggtgagcc tcatcgatca
    21421 gcagccaatg cggccggccg gttcgcattc gcatcgcgcg caggtgcggc agaagctccg
    21481 cgaaacgggc gggccgatcc gccagcggaa caccaaggag gttcacgttc gcgtgcacct
    21541 ccgggtcgcg cagaatggcc atgatttcgg cgatgccggg cgcgcgctgc tggtcgccga
    21601 tcgtgaccag catctgcagg ctcacatagt cgccttccgg atcgatcacg cacacctggt
    21661 agaagcgctc ggcgaagcgc tcgagaaggc cgcttgccag cgtcgacttg ccgccgcccg
    21721 aaggccccgc gatcagcacg ttgttgccat agggcggcag ccagaccgtg cggccgtcag
    21781 ggcgcgtgcc gagcgcaaca tggcggcgcg tgagccgcgc atcgatcgga ccgaggtcgt
    21841 tctcgatcag ctcgtcgatc agctcgacca caccctgccc ggccgcgcct gccgtcacga
    21901 atgccgccag acgcttgatc gaatcgaccg cgttcgcgac cgctaccgga cattcggcga
    21961 ggtgcatgaa ggaatgatcg ttctccgcgt caccgatcgc gaccagctca tggggcgaca
    22021 tgccgaggtt gcgcagcgcg accttcgttc cggaggcttt gttgataccg gcgggcagca
    22081 ccatgacggc ttcgcgattg aagatcactt gcagctcgag tccgagctcg cggatcgtct
    22141 ccatgacctg cgtttgctgt ggcgcatgcg tcgccacgat gaccttgccg acctcgaggg
    22201 gttgaacacc ccgccggcgc agtgccgcga tgaactgctc gggcggcgga tcggcgagca
    22261 gcgtggtgtc gcggcttcgc gggtcgtgaa cgagcgcacc gttctcggcg acgacgtaat
    22321 cgaagagctc ggcgcagtcg caaacacgca aaaggtcgtc cagcctgcgt ccggtcacga
    22381 ggatcgcgcg ccggccggat gcgcgcagcc tgccgagtgc tgcgcgggtc gcgtcgctga
    22441 ccctgccatc cgctgccagc gtgttgtcgt agtcgcttgc cagcgcaagg tatcgcattg
    22501 ccggcctcga tcagaattcg gttgacgtct gttcgtgcaa cgcttcgcgc caactgcgtg
    22561 agcgcccgcg ttcatcgttc tgcaaaccag gatgcgttgc gctgcacgag gacgacggct
    22621 gaacgcggcg ccacccggca agggcgggca gcgtcgacgg gcggttcctc gcccggctcg
    22681 aacaggtcgc ggggtgattc ggcacccgtt tcgacggcca gttgccatcg cgcaccatcg
    22741 gacgcaggcg gcacccggaa cggcgccgga tccgcgccgg cattgaacat gaggtaaagc
    22801 gcgctgccct cggcttcgtg gatcaggcag gccagccgcc tttctcgcgg atcggcccaa
    22861 gcgggcaatc ccaggtccgg atcgaaccag cgcatgtcgg cgtcggtgta gaagcgttct
    22921 tcgcacagaa ccgggtgcgc gcggcgaaag gcgatcattc cccgggtgaa ccggtaaatg
    22981 tcccgatgcc gctcgaggca gttccagtca t
//
LOCUS       DASSVX010000138        18931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_388739, whole genome shotgun sequence.
ACCESSION   DASSVX010000138 DASSVX010000000
VERSION     DASSVX010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 18931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18931
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388739"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VFO53_06020"
     CDS             <1..700
                     /locus_tag="VFO53_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8875869.1"
                     /translation="EHVSFDVRRGEIFVILGGSGSGKSTILKHMIGLNEPLAGSVIID
                     GADIVTASGDARRAVLRKIGVMYQSGALFGSMTLMENVRLPLEEYTDLAPGEIDLIAR
                     MKLSLVGLARFTGHLPAEISGGMQKRAAIARAMALDPMILFLDEPSAGLDPITSAELD
                     ALIKRLSRNLGVTFVIVTHELPSIFAIADRVIMIDRRVKGIVAEGDPRKLRDEAHDPW
                     VRQFFNREAERVPA"
     gene            870..1934
                     /locus_tag="VFO53_06025"
     CDS             870..1934
                     /locus_tag="VFO53_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET8875870.1"
                     /translation="MSRDANYFKLGLFVIGAVIAGIMVLTIIGTGRWLKPRTTIETYF
                     NESVQGLDLGSKMKYRGVVIGEVTRISFTYVVYEQDKPMNQRKRYVLVEAQIEPRLVG
                     GKAANDITSPESSKLEVERGLRIHLAPQGITGTNYLEVDYVDVPTASPVLPIDWVPDH
                     IYIPSTPSTVSTFVTAASDILDRLRRLDIESTIANLNKLLMTTNERVASLDTAGISQH
                     ADRTMSRIEATLDGLQSKKLSSEASGLLAELRQTNGELKTLLANPALAKLPGDTSATL
                     ASVRELVGNPGLPQSIAHLERTLSRLDRIFGAGEADLGTTFQNLRQITDNLRDLTEDA
                     KRYPSNLLFGAPPRQLEPAK"
     gene            1931..2536
                     /locus_tag="VFO53_06030"
     CDS             1931..2536
                     /locus_tag="VFO53_06030"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HET8875871.1"
                     /translation="MKLIPLFVVALLAGCSALTRQAPVRETFLLDPPAPAVIEKPQAG
                     TLRVGTINVAAPFRGKSFVYRVGELRFETDYYVEFLVPPAAMLAEQTARALDHAKAFA
                     RVAGPGTSAEASWVLDAFVSALYADRRDAGKPAAELDIEYFLTPEAENVQTPLWHHAY
                     RQRVAMRDASAAAYADALNKAFGDVLAELARDLAGATLPKP"
     gene            complement(2551..3915)
                     /gene="rimO"
                     /locus_tag="VFO53_06035"
     CDS             complement(2551..3915)
                     /gene="rimO"
                     /locus_tag="VFO53_06035"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET8875872.1"
                     /translation="MAPRLRTPEAPRVGFVSLGCPKALVDSEQVLTQLRAEGYAIAPS
                     YDGADLVVVNTCGFIDAAVAESLDAIGEALAENGKVIVTGCLGARDGGDFVRDAHPKV
                     LAVTGPHATHEVMSAVHAHLPKPHDPFVDLVPPQGIKLTPRHYAYLKISEGCNHRCTF
                     CIIPSMRGDLVSRPIGDVLAEAERLFRAGVRELLVVSQDTSAYGADVRYRTGFWQGRP
                     MKTRMLELARELDALANAHRAWVRLHYVYPYPHVDDVVPLMRSSPGVGGLVPYLDIPF
                     QHASPRILRLMKRPAAPEKVLDRVRAWRSTRPGIALRSTFIVGFPGETDAEFEELLAF
                     LREAQLDRVGCFAYSPVAGAAANALPNPVAEDVREARRARFMQVQAHISAARLRARIG
                     SDIDVLVDAVAGGIATGRSSADAPEIDGVVRITDGAALATGEFARVRVTSADEHDLVA
                     SRVA"
     gene            complement(4057..4632)
                     /gene="phaR"
                     /locus_tag="VFO53_06040"
     CDS             complement(4057..4632)
                     /gene="phaR"
                     /locus_tag="VFO53_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HET8875873.1"
                     /translation="MVSDIRTIKKYPNRRLYDTANSGYITLADVKQMVLDAIDFRVVD
                     AKSGDDLTRQILLQIILDEEAGGMPMFSSDMLSQMIRFYGTAQQTIIGQYIEQNVQAF
                     LAIQKKLQDQAKQVYGDKMMLTPELWKQFMQMQAPAMQGMLGNYLEQSAKLFIDMQQR
                     MQDQTRGMFSAFPFPTFGAQPPGTPDDDSKR"
     gene            4913..5464
                     /locus_tag="VFO53_06045"
     CDS             4913..5464
                     /locus_tag="VFO53_06045"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HET8875874.1"
                     /translation="MYNTSEQFAELNQAHVSNAIKLASLSIENAEKLVSLNINAAKLA
                     IAQAIEGAQAAASVKDVQELIALRAKLAEVGVQSATGYSRTLYELSSEAQATFSALAE
                     ESWKAYTKGMASWVERASQSAPAGSDVAVNALKSTFAASSAAFDQFQKATRQVVDLAD
                     ASVRAATDSAAKVAASTKVKRAN"
     gene            complement(5634..6611)
                     /locus_tag="VFO53_06050"
     CDS             complement(5634..6611)
                     /locus_tag="VFO53_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET8875875.1"
                     /translation="MQPAVVVRFALAVAFLFSSALAAAQESYPAHPITLVVPFPPGGV
                     ADIVGRPFADALSRELKTPVVIENKPGAGGGIGMGVVAKAKPDGYTLLLALSSISILP
                     EADKVLGRAPLYRLDQFAPIARLTADPTVLAVRADSRWNTLQEFIDDARKRPGAITYG
                     SSGNYGTMHMPMEMFAHVADIKLLHVPFTGGGPAVVALLGGQVDALSTGPSTVLQHVK
                     AGKVRVLASWGDHRLASLPAVKTLSESGYDAIFFQWSGLFAPVGTPAPIIARLREAAR
                     TAAADSRFVSTLATVETPVAYLDAPELRRFWDADAKKLADAVQRVGKVE"
     gene            complement(6717..8675)
                     /gene="copD"
                     /locus_tag="VFO53_06055"
     CDS             complement(6717..8675)
                     /gene="copD"
                     /locus_tag="VFO53_06055"
                     /inference="COORDINATES: protein motif:HMM:NF033808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HET8875876.1"
                     /translation="MNVVFVACRAVHFGAAMLVFGELAFAVFVAPAEWRSAIAGELDR
                     RRSLDRHVRAFTAWALAASAVSGIAWVALEASAMSGGGLMQVLADGTLAVVLRQTEFG
                     HVFAVRAVLLVGVAATLAWLWRASTRTQSRLRAVIALGLAGLYLATLAGAGHAAAATE
                     GVVRVVHLGADACHLLAAGGWIGALPALVHCFAHASSNAALAQLARRFSVLGIVCVAV
                     LMASGVVNTLFLVGSFAALFGTAYGNLLVVKLALFAAMLVLAAINRSRLTPRLADGDA
                     SAGRALRRNAMLEITIGVVIVAIVGALGTMVPGAHQSPVWPFAFAFDFSPATRSPEAS
                     GALWASLLLALAAIALIIAGVYRKSFRLWIPGCVALVVCAAASTSILAVPAFPTTYAS
                     SPVPYAVDAVARGAARYASDCSSCHGAGGRGDGPAAAGLSARPANLAEHALHHPPGNL
                     FWWIAHGIPGTPMPAFSPKLSDTQIWELVQFLVARASARSAQPLGPLVNGKSMSRAPD
                     FTYELPGEEQRTLNGQRTPALIVLYSLPQSKPRLTQLASDHRIMHANLRVLAIPLPGS
                     HPTDDANVPVDTIRDADVTAVYAMFAGTAGTSAAAHVELLVDATGIVRARWTGVPGTG
                     TDRDAQITAAAQRLPEPSRMPASMHHGR"
     gene            complement(8707..9081)
                     /locus_tag="VFO53_06060"
     CDS             complement(8707..9081)
                     /locus_tag="VFO53_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HET8875877.1"
                     /translation="MIPLLRRCVLLSIAAVACVAAAPAAAHAFLDHAFPAVGSTVHEA
                     PRSVRLWFTEQIEPAFSRVSVLDASGKEVDAGDSHVDSSDAAILAASLPVLAPGTYRV
                     KWRVVSVDTHVTEGDFTFDIRP"
     gene            complement(9078..10019)
                     /locus_tag="VFO53_06065"
     CDS             complement(9078..10019)
                     /locus_tag="VFO53_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875878.1"
                     /translation="MKLTPIRGAALVALCIAAPGIAEAHGFAGKRFFPATLATEDPFV
                     ADELSLPTIFHQNSPASGDEPASRETDLSVDMSKRITENFGIGLGTTYKIIHPDGGDT
                     QRGFDNLEASVKYKLYQNDMHEAIMSAGIDWDIGGTGARRVGAESFSTITPGVFFGKG
                     FGDLPAGMELLRPLAITGQVGVGIPTRSGTATTGDDGDIEIERNPHTLEWGFSIQYSL
                     PYLQSFVRDVGLEAPFNRMIPVIEFAMSTALDRGASGTTGTVNPGIIWAGQDIQLAVE
                     AVIPVNDRSGNSVGVLAQLHFFLDDLFPQTLGRPLIR"
     gene            complement(10126..10509)
                     /locus_tag="VFO53_06070"
     CDS             complement(10126..10509)
                     /locus_tag="VFO53_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875879.1"
                     /translation="MHSRAWHACIAWCALVAVAWPSLGPLPFLVHDFVPHVHRDGGHA
                     NAGDGDHFDASSIPGSPTHPVDHDCPECEVLKHLSRCVLPMLAVAIVAPVVVSVVTQR
                     VAVARPPAQIVAHLPPARAPPRSLA"
     gene            complement(10676..11071)
                     /locus_tag="VFO53_06075"
     CDS             complement(10676..11071)
                     /locus_tag="VFO53_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875880.1"
                     /translation="MKQSAIAICFAVAAAIGTPVLAHECRVRDGYIRGSFEGGCDEKA
                     GVANGYGEASSTDSYVGMFVDGHPDGKGVYTWHNGARLEGTFKAGKANGPGAYVSAKG
                     VRYDGPFENGKLTGAKRDDCPVTHGPLEC"
     gene            complement(11191..12855)
                     /locus_tag="VFO53_06080"
     CDS             complement(11191..12855)
                     /locus_tag="VFO53_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875881.1"
                     /translation="MHSYMKSKLRVSAVSAAVAAALGLVAGNAVAISQSGEAKNMIRV
                     GHTDLQGRPTYQPNVIKYPDGRTILFVGQHSGNPRPVAGYRFLPNPLNHNADENNGTM
                     IIDVTDPRNPVEKALIPAPVAGNSVGQSQMTRMCLGSQLPGGTPGKVYLLRNSQSGNL
                     AISGYQIWDVTDVEHPVKTSEQMGLRSTHKVWWECSTGIIYAPGSLSTPPLWKESQAM
                     LVYDWHDVNNPPHFIRMFGLPGGEPGGTVTTSLHGAISAHDHPMAAQKLARADDVIGN
                     RIYAAWGVGDDGVITIIDRKKLLPKEYGGTWVPATADSATQPLETELIGPNSPTVGYF
                     TMSPDQGGHTSMPVFGIKPPSYGKYNEFQTRDVVVVTSEATADGKDGRCNEAPHWAFT
                     LDVTVENSMSVPPGTKVERDPYQGPMVLGTMSVDPRSGEQSKRGNFCERGARFGVHSV
                     EENFNNPYYGKLTAIAYFNGGIRIWDIREPQAPREVAFYVPEADALTDPDGYMTNNVE
                     IDNRGYIYVVDRNGAGMDILELNGCAKQIADNGQTCPDLGNNNAAR"
     gene            complement(12914..13420)
                     /locus_tag="VFO53_06085"
     CDS             complement(12914..13420)
                     /locus_tag="VFO53_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875882.1"
                     /translation="MNVASSPLPPHPSIARIPSGRRRPKRLAIARISISIVVALSAAQ
                     ASGEIYKCTARKSIPKYQNFPCEFDTLGSVPAASAPETDSRTTGATAAARTATPTRPD
                     RNVMHRGAGASVPRIGMTTDEVRAVWGEPVETNKEEFVKGTFETWTYADSRAVQFDRK
                     GRVTSIKR"
     gene            complement(13602..14546)
                     /locus_tag="VFO53_06090"
     CDS             complement(13602..14546)
                     /locus_tag="VFO53_06090"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET8875883.1"
                     /translation="MRDCDVAAELSARGTTPVLQPARPRPRAATSVLSGTIPDPLLSS
                     RARGWDGLTVELQSFHDLDVVVQSSDHVIAVQLAGSVNVHQSRAGRTRSRTISVGDVT
                     VTPVGPPTRWRQAGQSLVVLLRLSPAYVRGVAGDECALDPDRFEIQGRFATRDARIDD
                     VARRLLAGLELEGGDSHLYVDALTCELAIHLLREYTVGSVAPAWPMARLSPHKLRRAI
                     EYIDDNLKGELTLAAIADTVALSPGHFAHAFRQAIGVAPHRYVVERRVERAKALLRGS
                     DMPITEIADRVGCASHSHFSVLFHRVTGLTPRQFRALE"
     gene            complement(14560..15306)
                     /gene="phbB"
                     /locus_tag="VFO53_06095"
     CDS             complement(14560..15306)
                     /gene="phbB"
                     /locus_tag="VFO53_06095"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HET8875884.1"
                     /translation="MAAQRIALVTGGMGGLGEAICTKLSALGNTVVTTHSPGNTKSAD
                     WLEAMKRQGYDFRAYACDVAHFDSASACVRQVTNELGPIDVLVNNAGITRDMTFRKMD
                     KANWDAVIGTNLDSCFNMTKQVCDGMTERGWGRIINISSVNGQKGAFGQTNYSAAKAG
                     MHGFTKALALEVARKGVTVNTISPGYIGTKMVMAIPKDVLDSKIIPQIPMGRLGKPEE
                     VAGLVAYLASDEAAFLTGANIAINGGQHMF"
     gene            15513..16766
                     /locus_tag="VFO53_06100"
     CDS             15513..16766
                     /locus_tag="VFO53_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011337705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET8875885.1"
                     /translation="MKFSMKAVAVLCALAICPIAGQADELETLSGMHQTNTPVDWPMI
                     PQTGAKADQIKQNLTKIKLPPGFHIGLYAIVPDARHIAVGPQGIVTFVGTRKTKVWAV
                     TDRSRGGVADEVKEFAPTLPKRIPNGPCFSKDGFLYVVEQNRVVEYPAAEFFYESPDV
                     VPNAIVPEGKLIPKEEESYNHTARPCRIGPDNKLYIQLGQPYNVPPKEKLDLYHKTGM
                     MGIIRMDRDGKNREVYATGLRNAVGMDFNPKDKTLWSNDNQVDGMGDDQPPGELNRIA
                     KQGLDFGFPWYGGGHTRTTEYKNDTPPAGVVFPEVEQIAHAADLGLLFYTGTMFPAKY
                     RGAIFSTQHGSWNRTVPAGARVLVTFVKEDGHVAGKSEPFAEGWNDNGYYLGRPVDVA
                     ELHDGSLLVSDDLVGALYRIWYDGK"
     gene            16763..17104
                     /locus_tag="VFO53_06105"
     CDS             16763..17104
                     /locus_tag="VFO53_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET8875886.1"
                     /translation="MMRASAALVGAAALLCFAAPEARAAGDAKAGRMKAQMCQACHGT
                     DGLSQVPDAPNIAGQVEGYLVAQLQAFKSGARKNEAMTLVAAGLSSQDIDNLAAWYSS
                     IEIKLGKLPGG"
     gene            complement(17113..18828)
                     /gene="phaC"
                     /locus_tag="VFO53_06110"
     CDS             complement(17113..18828)
                     /gene="phaC"
                     /locus_tag="VFO53_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HET8875887.1"
                     /translation="MAVPEPHTIESWLALAAQWQQALQSWTAWWAGAGRTVPGAEASA
                     VATTIRAPFDAEGVAALHAAFAPRVQALWAAPAQARAPADDRRFASAAWRERPYFAWL
                     AQSYRLYGEFLTALASIAQLPPDEKKRLEFSVRQFVDAIAPTNFPATNPDVLARAIET
                     EGASVVDGLRNLAADVAKGRITMSDESAFEVGRNLAVTPGSVVLRNDLIELIQYDATT
                     PRVARRPLLIVPPCINKYYILDLKPANSFVRHAVEQGHTVFMISWRNVPAELGTLSWD
                     DYLAQGVLAALTAVKAISRSRDVNTLGFCVGGTLLACALAVLAARNDTSASSVTFLTT
                     MLDFADPGEIGVYVSRQMLAAREAQLAAGQRVHGSELAGAFASLRPNDLVWNYVVDNY
                     LKGRKPPAFDLLYWNGDSANLPGPMYAYYIRQLYLENRLRERGALTMLGEKIDLSRVA
                     LPAYVYASRDDHIVPWRSAFRTLDLVGGETTFVLGASGHIAGVVNAPEAHKRNYWTHD
                     GRERSTDAWVANATSHTGSWWPHWYRWLAQHRGGERAAPKRTGNATHRPLARAPGEYV
                     VERVS"
BASE COUNT         3262 a         6194 c         6323 g         3152 t
ORIGIN      
        1 cgagcacgtg agcttcgacg tgcggcgcgg tgaaattttc gtcatcctcg gcggatcggg
       61 cagcggcaag agcaccatcc tcaagcacat gatcggcctc aacgagccgc tggccgggag
      121 cgtgatcatc gacggcgccg atatcgtgac ggcgagcggc gacgcgcggc gcgcggtcct
      181 gcgcaagatc ggtgtcatgt accagtcggg cgcactgttc gggtcgatga cgctgatgga
      241 gaacgtgcgg ctgccgctcg aggaatatac cgacctcgcg ccgggcgaga tcgatctcat
      301 cgcacgcatg aagctttcgc tggtgggcct tgcgcgcttc accggtcacc tgccggccga
      361 aatctcgggc ggcatgcaga aacgtgcggc gatcgcgcgc gccatggcgc tcgatccgat
      421 gatcctgttc ctcgacgagc cttcggccgg gctcgatccg atcacgtccg ccgagctcga
      481 cgcgctgatc aagcggctgt cgcgcaacct cggcgtgacg ttcgtgatcg tcacccatga
      541 gctgccgagc atcttcgcga tcgccgatcg ggtgatcatg atcgaccggc gcgtcaaggg
      601 catcgtcgcc gagggtgatc cgcggaagtt gcgtgacgag gcgcacgatc cgtgggtgcg
      661 ccagttcttc aaccgcgagg cggagcgggt gccggcatga acgcttccat cgagcatcac
      721 gcaactccat gaccattgca aagcaccgtc gttcccgcgg aagcgggaac cccgtgtctt
      781 tcgctcgaac gcgccactgg gtccccgcgt tcgcggggac gacggagctt cgggagcccg
      841 gcgtcacgcg atcaggtaag gctcgataaa tgagccgcga cgcgaactat ttcaagctgg
      901 gcttgttcgt gatcggcgcg gtgatcgccg gaatcatggt gctcacgatc atcggcaccg
      961 gacgctggct gaagccccgc acgacgatcg agacgtattt caacgagtcg gtgcagggcc
     1021 tcgatctcgg ctcgaagatg aaatatcgcg gcgtggtgat cggcgaggtc acgcggatca
     1081 gcttcactta tgtggtttac gaacaggaca agccgatgaa ccagcgcaag cgttacgtgc
     1141 tggtcgaggc gcagatcgag ccgcgcctgg tcggcggcaa ggccgcgaac gacatcacga
     1201 gccccgaatc gagcaagctc gaggtggagc gcggcttgcg cattcatctt gcgccgcagg
     1261 gcatcacggg caccaactat ctcgaggtcg attacgtgga cgtgccgacg gcgtcgcccg
     1321 tgctgccaat cgactgggtg cccgaccaca tctacatccc gagcacgccg tcgacggtgt
     1381 cgacgttcgt caccgccgct tcggacatcc tggatcggct gcggcggctc gacatcgagt
     1441 cgacgatcgc caatctcaac aagcttctga tgacgacgaa cgaacgcgtg gcgagcctcg
     1501 acacggccgg catttcgcag cacgccgatc gcacgatgtc gaggatcgag gctacgctcg
     1561 acggcctgca gtcgaaaaag ctgtcgagcg aagcgagcgg cctgcttgcc gagttgcggc
     1621 agacgaacgg cgagctcaag acgctgcttg cgaatccggc gctcgcgaaa ttgcccggcg
     1681 atacgtcggc cacgctcgcc agcgtgcgcg agctcgtcgg caatcccggc ttgccgcagt
     1741 cgatcgcgca tctcgagcgc acgctttcgc ggctcgaccg catcttcggc gccggcgaag
     1801 ccgatctcgg cacgaccttc cagaacctgc gccagatcac cgacaacctg cgcgatctca
     1861 ccgaagacgc gaagcgctac ccgtcgaacc tgctgttcgg cgcgccgccg cgtcagctgg
     1921 agcctgcgaa atgaagctga ttccgctgtt cgtcgtggcg ctgctcgccg ggtgctcggc
     1981 gctcacgcga caggcgccgg tgcgcgagac gttcctgctc gatccacccg cgccagcggt
     2041 gatcgagaag ccgcaggccg gcacgttgcg cgtcggcacg atcaacgtcg cggcgccgtt
     2101 tcgcggcaag agcttcgttt accgcgtggg cgagctgcgc ttcgagaccg actactacgt
     2161 ggagttcctc gtcccaccgg cagcgatgct ggccgagcag acggcgcgcg cgctcgacca
     2221 cgccaaggcg ttcgcgcgtg ttgcgggccc ggggacgagc gcggaagcgt cgtgggtgct
     2281 ggacgcgttc gtctcggcgc tctacgccga caggcgcgat gccggcaagc ccgccgccga
     2341 gctcgacatc gagtatttcc tgacacccga agcggagaac gtgcagacgc cgctgtggca
     2401 ccacgcatac cgccagcgcg tcgcgatgcg cgacgcaagc gccgccgcct atgccgatgc
     2461 gctgaacaag gcgttcggcg acgtgcttgc ggaactcgcg cgcgatctcg ccggggccac
     2521 cctgccgaag ccctgagcgc agccgtgggg tcaggcgacc cgcgaggcga cgagatcgtg
     2581 ttcgtcggcg gacgtgaccc gcacgcgtgc gaattcgccg gtcgcaagtg ccgcgccgtc
     2641 ggtgatgcga acgacgccgt cgatctccgg cgcatcggca ctcgagcgtc cggtggcgat
     2701 gccgccggca accgcatcga cgaggacatc gatatcgctt ccgatcctcg cacgcaaccg
     2761 cgccgcactg atatgcgcct gcacctgcat gaaccgcgcg cgccgcgctt ccctcacgtc
     2821 ttcggccacc ggattcggta gcgcattcgc cgccgcgccg gcgaccggcg aatacgcgaa
     2881 gcagccgacg cggtcgagct gcgcttcgcg caggaacgca agcagttcct cgaactcggc
     2941 atccgtttcg ccgggaaagc cgacgatgaa cgtgctgcgc agtgcgatgc cgggccgcgt
     3001 cgaacgccag gcgcgcacgc gatcgaggac cttctccggc gccgccggcc gcttcatcag
     3061 ccgcaggatg cgcgggctcg cgtgctggaa cgggatgtcc agatagggca cgaggccgcc
     3121 aacgcccggc gacgagcgca tcagcggaac gacgtcgtcg acatgcggat acggatagac
     3181 gtaatgcagc cgcacccatg cgcgatgcgc attcgcgagc gcgtcgagct cgcgggcgag
     3241 ctccagcatg cgcgtcttca tcggccggcc ctgccagaag ccggtgcggt agcgcacgtc
     3301 ggccccgtaa gcgctcgtat cctgcgagac gacgagcaat tccctgaccc ccgcgcgaaa
     3361 caggcgctcg gcttcggcga gcacgtcgcc gatcgggcgc gagacgagat cgccgcgcat
     3421 cgacggaatg atgcagaacg tgcagcggtg gttgcagccc tcgctgatct tcaggtacgc
     3481 gtaatggcgc ggcgtgagct tgatcccctg cgggggcacg agatcgacga acggatcgtg
     3541 cggcttcggc agatgcgcat gcaccgccga catgacttcg tgcgtcgcgt gcggacccgt
     3601 aacggcaagc accttcggat gcgcgtcgcg cacgaagtcg ccgccatccc tggcccccag
     3661 gcagccggtc acgatgacct tgccgttctc ggcgagcgct tcgccgatcg cgtcgagcga
     3721 ttcggcaacc gccgcgtcga tgaagccgca cgtgttgacg acgacgagat ccgcaccgtc
     3781 gtacgatggc gcgatcgcgt acccttcggc gcgcagttgc gtcagcacct gctcggaatc
     3841 gacgagcgcc ttcgggcagc cgagggaaac gaagcccacg cgcggcgctt cgggagtgcg
     3901 gagacggggt gccatggcga gggtcgatcg ttggatgcgg ggtggtcaga agcgcgcttc
     3961 gcagcgcagc aagcgaagtg tgcaacgcgc catgttgcgc cgcggcgcga gaaactcaac
     4021 gtccggtatg cgtcgggcgc cggcggctcg cgccgctcat cgcttcgagt cgtcgtcggg
     4081 cgtgcccggc ggctgcgcgc cgaacgtggg gaacggaaag gccgaaaaca tgccgcgcgt
     4141 ctggtcctgc atgcgctgct gcatgtcgat gaagagcttc gcgctctgct cgagatagtt
     4201 gccgagcatg ccctgcatcg ccggcgcctg catctgcatg aactgcttcc acagctcggg
     4261 cgtcagcatc atcttgtcgc cgtagacctg cttcgcctgg tcctgcagct tcttctggat
     4321 cgcaaggaac gcctgcacgt tttgctcgat gtactggccg atgatcgtct gttgcgcagt
     4381 gccgtagaag cggatcatct gcgacagcat gtccgagctg aacatcggca tgccgccggc
     4441 ttcctcgtcc aggatgatct gcagcagtat ctggcgcgtg agatcgtcgc cgctcttggc
     4501 gtcgacgacg cggaagtcga tggcgtcgag caccatctgc ttcacgtcgg cgagcgtgat
     4561 gtagccgctg ttggccgtgt cgtacagccg gcggttgggg tacttcttga tcgtgcggat
     4621 gtccgacacc atcgatgcgc tgccttccac ggagcacgca ttataacccg ctgatttgtc
     4681 ggctctttgg aatcgtttcc ggcggggccg cggtcgtatg ctgcggtgca caaaaaaggc
     4741 ttgacttcgg agacttccga ccctacaatg agttttgtgc ggtgcagcat aatcgggtgt
     4801 cgcggagaac tttccacccc ggtagccgac accggattcg ggcgggaaaa gcgtttacac
     4861 ggttcctgcc aacggcaaat cgggtcgtcg acccgccatt gaaagggtaa ccatgtacaa
     4921 cacatcggag caattcgcgg aactgaatca ggcgcacgtc tcgaacgcga tcaaactcgc
     4981 gtcgctgtcg atcgagaacg ccgagaaact cgtcagcctc aacatcaacg cagccaagct
     5041 cgcgatcgcg caggcgatcg aaggcgcgca ggccgccgca tcggtcaagg acgtgcagga
     5101 actgatcgcg ctgcgcgcca agctcgccga agtcggcgtg caaagcgcaa ccggatactc
     5161 gcgcacgctg tacgagctgt cgtccgaagc gcaagccacg ttctcggcgc tcgccgagga
     5221 aagctggaag gcttacacga agggcatggc gtcgtgggtc gagcgcgcga gccagtcggc
     5281 gcccgccggc tcggatgtcg cggtcaacgc actgaagtcg acgtttgcag cttcgtcggc
     5341 cgccttcgac cagttccaga aggccacgcg tcaagtcgtc gacctggccg atgcgagcgt
     5401 tcgcgccgcg acggattcgg cggcgaaggt ggccgccagc acgaaggtca agcgcgcgaa
     5461 ctaacgcggc gacgtttcgg cattgcgggg cggcggcgct tgaacgatgg cggcgccatc
     5521 cccagatcaa caacaggatg gtgatgcctc ctctgtatca attctctcct cctgattgat
     5581 acagcattca accccgcagg cttccagccc gcggggtttt ttttacgcgt ttctcattcc
     5641 accttgccga cgcgctgcac ggcgtcggcg agcttcttcg catccgcgtc ccagaagcga
     5701 cgcagctcgg gtgcatcgag ataggcgacc ggcgtctcga cggtcgccaa tgtgctcacg
     5761 aaacgcgagt cggcggccgc agtgcgcgcg gcctcgcgca gccgcgcgat gattggcgca
     5821 ggcgtgccga ccggcgcgaa cagtcccgac cactggaaga agatcgcgtc gtagccggat
     5881 tcggacagcg tcttcaccgc gggcagcgac gcaagccgat gatcacccca tgacgcgagg
     5941 acacgcacct tgcctgcctt gacgtgctgc aggacggtgg acggccccgt cgacagcgcg
     6001 tcgacctgcc cgccgaggag tgcgaccacg gcaggtccgc cgcccgtgaa cggaacgtgc
     6061 agcagcttga tgtcggcgac atgcgcgaac atctccatcg gcatgtgcat cgtgccgtag
     6121 ttgcccgacg atccgtaagt gatggcgccg ggacgcttgc gcgcgtcgtc gatgaactcc
     6181 tgcagcgtgt tccaacggct gtccgcgcgc acggcgagta cggtcgggtc ggcggtaagc
     6241 ctcgcaatcg gcgcaaactg gtcgagccgg tagagcggtg cgcggccgag gaccttgtcc
     6301 gcctccggca ggatcgagat cgacgacaac gcgagcagca gcgtgtagcc gtcgggcttc
     6361 gccttcgcaa ccacgcccat gccgatgccg ccacccgcgc ccggcttgtt ctcgatgacg
     6421 accggcgtct tcaattcgcg cgacagcgcg tcggcgaacg gccgcccgac gatgtcggcg
     6481 accccgcccg gcggaaacgg caccacgagc gtgatcggat gggcggggta gctctcctgc
     6541 gccgccgcca gcgcggacga gaacaggaac gcgacagcga gcgcgaagcg cacgacgacg
     6601 gcaggttgca tgctgaaacc tcggcaggga gcggttggag actcgtttgc gaagcgcacg
     6661 atagaacgcg gcacggcgac gcgtcaatcg ctcgacaacg tcgagcgact tctgcgtcag
     6721 cgcccgtgat gcatcgacgc cggcatccgc gatggctcgg gcagccgttg tgccgccgca
     6781 gtgatctgcg cgtcgcgatc ggtgccggtg cccgggacgc cggtccatcg cgcgcgcacg
     6841 atgcccgtgg catcgaccag cagctcgaca tgcgcggcgg cggaagtccc tgcggtgccc
     6901 gcgaacatcg catacaccgc ggtaacgtcc gcatcgcgga tcgtgtcgac gggcacattc
     6961 gcatcgtcgg tgggatgcga gcccggcagc gggatcgcga gcacgcgcag gttcgcatgc
     7021 atgatccggt ggtcggaagc gagctgcgtc aaccgcggct tcgattgcgg cagcgaatac
     7081 agcacgatca gcgcgggcgt tcgttgcccg ttcagcgtcc gctgttcctc gcccggcaac
     7141 tcataggtga aatcgggcgc gcggctcatc gatttgccgt tcaccagcgg tcccagcggc
     7201 tgcgccgacc gagcactcgc gcgggcgacg aggaactgca cgagctccca gatctgcgtg
     7261 tcggatagct ttggcgagaa cgccggcatc ggcgttcccg gaatgccgtg cgcaatccac
     7321 cagaaaaggt tgccgggcgg atgatgcagc gcgtgctcgg caaggttggc cggccgcgcc
     7381 gaaagcccgg cagccgccgg cccatcgcca cgcccgcccg cgccatggca actactgcag
     7441 tcgctcgcgt agcgtgcggc gccgcgcgca accgcgtcga ccgcgtacgg caccggcgaa
     7501 ctggcgtagg tcgtggggaa cgccggcacg gcaagaatcg acgtcgacgc cgcggcgcaa
     7561 acgaccagcg cgacgcagcc gggaatccac agccggaaag actttcgata cacgccggcg
     7621 atgataagcg cgatcgcggc aagcgcaagc agaaggctcg cccacagcgc gccgctcgcc
     7681 tccggcgacc gcgtcgccgg cgagaaatcg aaggcgaacg cgaacggcca taccggggat
     7741 tgatgggcgc cggggaccat cgtcccgagc gcgccgacga tcgcgacgat gacgacgccg
     7801 atcgtgatct cgagcatcgc gtttcggcgc aacgcgcgtc ctgccgatgc gtcgccgtcc
     7861 gcgagacgcg gcgtcaatcg cgaccggttg atggcggcga gcacgagcat cgccgcgaaa
     7921 agcgcgagct tgacgacgag cagattcccg tacgcggtgc cgaagagtgc ggcgaacgag
     7981 ccgacgagga acagcgtgtt gacgacgccg ctcgccatca acacggcgac gcagacgatg
     8041 cccagcacgg aaaagcgtcg tgcgagctgc gcaagcgccg cattcgacga cgcgtgcgcg
     8101 aaacaatgga cgagtgccgg cagcgcgccg atccagccgc ccgcggccag caaatggcat
     8161 gcatcggcac cgaggtgcac gacacgaacg acaccttcgg tcgctgcggc cgcgtgtccg
     8221 gcacccgcaa gggtcgcgag atagaggccc gccaacccca gtgcgatcac ggcacgaagc
     8281 cgtgattgcg tgcgggtcga tgcgcgccac agccaggcga gggtcgccgc gacgccgacg
     8341 agcagcaccg cccgcaccgc gaagacatgg ccgaattccg tctgccgcag cacgacggcc
     8401 agcgtgccat cggccaggac ttgcatgagg ccgccgccgg acatcgcgga cgcttcgagc
     8461 gcgacccacg cgatgcccga caccgcgctc gccgcaagcg cccacgcggt gaacgcgcgc
     8521 acgtgccgat cgagactgcg tcgtcgatcg agctcgccgg cgatggccga ccgccattcg
     8581 gccggcgcaa cgaagacggc gaacgcgagc tcgccgaaca cgagcatcgc ggcgccgaaa
     8641 tgcacggcgc ggcaggcgac gaagacgacg ttcatcgcgt gcccggctgg cggtcaacgg
     8701 agagcatcag ggtcttatgt cgaacgtgaa gtctccttcc gtcacgtgcg tatcgacgga
     8761 gacgacgcgc catttgacgc ggtacgtgcc cggcgcaagc acgggcaggc tcgcggcgag
     8821 gattgcggca tcggatgaat cgacgtgcga atcaccggcg tcgacctcct tgccggatgc
     8881 gtcgagcacg ctgacgcgac tgaacgccgg ctcgatctgc tcggtgaacc acaggcgcac
     8941 gctgcgcggc gcttcgtgca cggtgctccc gacagcgggg aacgcgtgat cgagaaacgc
     9001 gtgcgccgcc gccggcgccg cggcaacgca tgcgacggcg gcaatcgaca aaagcacgca
     9061 acggcggagc aatgggatca tcggatgagc gggcggccga gcgtctgcgg gaacaggtcg
     9121 tcgaggaaga aatgcagctg cgcgagcacg cccacgctgt tcccgctgcg atcgttgacc
     9181 ggaatcaccg cttcgactgc caattggatg tcctgcccgg cccagatgat cccgggattg
     9241 acggttcccg tcgttccgct cgcgccgcgg tcgagcgcgg tcgacattgc gaactcgatc
     9301 accgggatca tccggttgaa cggcgcctcg agcccgacgt cccgcacgaa cgattgcagg
     9361 tagggcaggc tgtactggat cgagaagccc cactcgagcg tatgcggatt gcgctcgatc
     9421 tcgatgtcgc cgtcgtcacc ggtcgtcgcc gtgccgcttc gcgtcggaat gccgacgccc
     9481 acctgtccgg tgatcgcaag cgggcgcagc agctccatcc cggccggaag atcgccgaag
     9541 cccttgccga agaacacgcc cggcgtgatc gtgctgaacg actcggcgcc gacgcgccgc
     9601 gcgccggtgc ctccgatgtc ccagtcgatg ccggccgaca tgatcgcctc gtgcatgtcg
     9661 ttctgataga gcttgtactt gacgctcgcc tcgaggttgt cgaaaccccg ctgcgtgtca
     9721 ccgccgtcgg gatggatgat cttgtacgtc gtgccgaggc cgatgccgaa gttctcggta
     9781 atccgcttcg acatgtcgac cgagagatcg gtctcgcgcg atgccggctc gtcgccgctc
     9841 gccggcgagt tttggtgaaa tatcgtcggc agcgacagct cgtccgcgac gaacgggtcc
     9901 tcggttgcga gcgtcgcggg gaaaaaccgc ttgcccgcga atccatgcgc ctcggcgatg
     9961 cccggcgccg cgatgcacag tgcgacgagc gccgcaccgc gaatgggtgt caacttcact
    10021 ttgcttcctc cagtttgcga acgaatgcgg cggccgtacg cgccgcgcgc gaaaccgctc
    10081 gaaaggcggg tcgcgcgggc ggccggcggc cggttcagcg gcgaatcacg cgagcgaacg
    10141 cggaggagcg cgtgccggag gaaggtgcgc aacgatctgc gcgggcggcc gggcaacggc
    10201 gacgcgttgc gtgacgacgc tcacgacgac cggcgcgacg atcgcgacgg cgagcatggg
    10261 cagcacgcat cgcgaaaggt gcttcaggac ttcgcattcg ggacaatcgt ggtcgaccgg
    10321 atgcgtcggg ctgccgggga ttgacgaggc gtcgaagtga tcgccgtcgc ctgcgttcgc
    10381 gtggccgccg tcccgatgga cgtgcgggac gaaatcgtga acgaggaacg gcagcgggcc
    10441 gagcgagggc cacgcaacgg cgacgagcgc gcaccaggcg atgcacgcgt gccacgcgcg
    10501 ggagtgcacc ggtcgtcgcc tggttcgggc gcgcatcgtg ttcggcagtg gctgcgcttg
    10561 cggcagcgat gacgcgagga tacgcggttg ttcggttgcc tcgtgtggcc tgactaaacc
    10621 ggattgcgga gcggaggaat gcacctcgcc cccgtggggc tgcgtagcgc cgcgctcaac
    10681 actcgagcgg gccgtgcgtc accggacaat cgtccctttt tgcacccgtc agcttcccgt
    10741 tctcgaacgg cccgtcgtaa cgcacgcctt tcgccgagac atacgctccc ggcccgttgg
    10801 ccttcccggc cttgaacgtc ccttcgagac gcgccccgtt gtgccacgtg tagacgccct
    10861 tcccatcggg gtgtccatcg acgaacatgc cgacgtacga gtcggtggag ctcgcttcgc
    10921 cgtaaccgtt ggcgaccccc gccttttcgt cgcagccgcc ctcgaagctt ccccggatgt
    10981 aaccgtcccg caccctgcac tcgtgcgcga ggacgggcgt gccgatggct gcagccacgg
    11041 cgaaacagat tgcgattgcc gattgcttca tcgatccctc ggggcaaaaa acggctcgac
    11101 gcgttgcgcc gagccgttgc tggaacaaag acaggggtgc gcgattgatg caaggccccg
    11161 cggcgcgaac gccgcggggg gagcgttcct ttacctggcg gcgttgttgt tgccgagatc
    11221 ggggcaggtc tgaccgttgt cggcgatctg cttcgcacac ccgttgagct cgaggatatc
    11281 catgccggcg ccgttgcggt cgacgacgta gatgtagcct cgattgtcga tctccacgtt
    11341 gttggtcatg tagccgtcgg ggtccgtaag cgcatccgct tccggcacgt agaacgccac
    11401 ttccctcggc gcctgcggct cgcggatatc ccagatgcgg atgccgccat tgaaatacgc
    11461 gatcgccgtc agcttgccgt agtacgggtt gttgaagttc tcctcgaccg agtgaacgcc
    11521 gaagcgtgcg ccgcgttcgc agaaattccc gcgcttgctc tgctcgcccg accgcggatc
    11581 gaccgacatc gtgccgagca ccatcgggcc ctggtacggg tcgcgctcga ccttggtgcc
    11641 cggcggcacg ctcatcgaat tctcgacggt gacgtcgagc gtgaacgccc agtggggcgc
    11701 ctcgttgcag cggccgtcct tgccatcggc cgtcgcttcc gaggtcacga cgacgacatc
    11761 gcgcgtctgg aactcgttgt acttgccgta gctcggcggc ttgatgccga agacgggcat
    11821 cgacgtgtgg cccccctggt cgggcgacat cgtgaagtag ccgacggtcg gcgagttcgg
    11881 gccgatcagc tctgtttcga gcggctgggt tgcgctgtcg gcggtcgccg gcacccacgt
    11941 cccgccgtac tccttcggca gcagtttctt gcggtcgatg atcgtgatca cgccatcgtc
    12001 gccgacgccc cacgccgcat agatgcggtt gccgatgacg tcgtcggcgc gcgccagttt
    12061 ctgcgcggcc atcggatggt catgcgcgga aatcgcaccg tgcagcgacg ttgtcaccgt
    12121 gccgcccggc tcgccgccgg gcaggccgaa catgcggatg aaatggggcg ggttgttgac
    12181 gtcgtgccag tcgtagacga gcatcgcctg cgattccttc cacagcggcg gcgtgctcag
    12241 gctacccggc gcatagatga tgccggtgct gcattcccac cagaccttgt gcgtcgagcg
    12301 gagccccatc tgctccgacg tcttgaccgg gtgctcgacg tcggtgacgt cccagatctg
    12361 gtagccggag atcgcaaggt tgccggattg ggagttgcgc agcaggtaga ccttgcccgg
    12421 cgttccgccc ggcaactgcg atccgaggca catgcgcgtc atctgcgact ggcccacgga
    12481 attccctgcg acgggcgccg gtatcagcgc tttctcgacc gggtttctcg gatcggtgac
    12541 gtcgatgatc atcgttccat tgttctcgtc cgcgttgtgg ttgagcggat tcggaaggaa
    12601 cctgtagccc gccacgggcc gggggttgcc gctgtgctgc ccgacgaaca ggatggtccg
    12661 tccgtcgggg tatttgatca cgttcggctg atacgtcgga cgtccctgca ggtcggtatg
    12721 gcccacgcgg atcatgttct tcgcttcccc gctctggctg atcgccaccg cattgccggc
    12781 gacgaggccg agcgctgccg cgacggcggc ggatacggcg cttacacgca gcttgctctt
    12841 catgtacgaa tgcattgctc ctccttgggg ttaaccgccg gctcgccctc gcgacgatgc
    12901 cttcggccga caactaccgt ttgatgctcg ttactcgacc cttgcggtcg aattgaaccg
    12961 ctcgcgaatc ggcgtacgtc cacgtctcga aagtgccttt gacgaattcc tccttgttcg
    13021 tttcgacggg ttcgccccag acggctctca cctcgtcggt tgtcatgccg atgcggggca
    13081 ctgacgcgcc ggcgccgcga tgcatgacgt tgcggtccgg ccgggtcggc gtcgcggtgc
    13141 gggccgcggc tgtcgcgcct gtcgtgcggg aatcggtctc gggcgcggag gcggccggca
    13201 ccgacccgag cgtgtcgaat tcacagggaa aattctgata cttggggatc gacttccttg
    13261 ccgtgcactt gtagatctcg cccgaggcct gcgcggcgga aagcgcaacg acgattgaaa
    13321 tcgaaatccg ggcaatcgcg agccgcttcg gccgtcgccg gcccgacggg attcgcgcga
    13381 tgctcgggtg cggcggcaaa ggcgacgatg cgacgttcac gagtggatcg acaaatgaat
    13441 ccgggaaacg aatctagccg cgcgcagccg gtgtgcgctt tcacgttctt gcggaaactt
    13501 ttcgaatcgt gcgattcgtt tgttgcaggc agcgcaaagg cgcgcgatgc aatatttgca
    13561 atgcgctggc tgttgcgagc gcgcccacgc ccgcgcgacc gttactccag cgcgcgaaac
    13621 tgccgcggcg tgagcccggt gacgcgatgg aagagaaccg agaagtgact gtggctcgcg
    13681 cagccgacgc gatcggcgat ctcggtgatc ggcatgtccg agccgcgcag cagcgccttt
    13741 gcgcgttcga cgcgccgctc gaccacgtag cggtgcggtg cgacgccgat tgcctggcgg
    13801 aacgcatgcg cgaaatgacc cggactcaat gcaaccgtgt cggcgatcgc ggccagcgtc
    13861 agttcgcctt tcaggttgtc gtcgatgtac tcgatcgcgc ggcgcagctt gtgcggcgaa
    13921 aggcgcgcca tcggccaggc gggtgcgacg ctccccaccg tgtattcgcg cagcaggtgt
    13981 atcgcgagct cgcacgtgag cgcatcgacg tagagatgac tgtcgccgcc ttcgagttcg
    14041 agtcctgcga ggaggcgccg ggcaacatcg tcgatgcgcg cgtcacgcgt cgcgaagcgc
    14101 ccctgtattt cgaagcggtc gggatcgagt gcgcattcgt caccggccac gccgcgcacg
    14161 tacgcgggcg acaatcgcag caatacgacg aggctctgtc ctgcctggcg ccagcgggtc
    14221 ggaggtccca ccggcgtgac ggtgacgtcg cccacgctga tcgtccgcga tcgcgtccgc
    14281 ccggcgcgac tctgatgcac gttcacgctg cccgccagtt gcacggcaat cacgtgatcg
    14341 gacgactgca cgaccacgtc gagatcatgg aagctctgca actcgaccgt cagcccgtcc
    14401 cacccgcgag cgcggctcga cagcagcgga tcgggaatgg tgccggacag caccgacgtc
    14461 gccgcgcgtg gtcgcgggcg ggcgggctgc aggacgggag tcgttccgcg ggcggagagc
    14521 tccgccgcaa cgtcgcaatc gcgcattgcc gcctaccctt cagaacatgt gctggccgcc
    14581 gttgatcgcg atgttcgcgc cggtgaggaa ggcggcttcg tcgcttgcaa gatacgcgac
    14641 gagtcctgcg acctcttcgg gcttgccgag gcgccccatc gggatctgcg gaatgatctt
    14701 gctgtccaga acgtccttcg ggattgccat caccatcttc gtgccgatgt acccgggcga
    14761 aatcgtgttc accgtgacgc ccttgcgcgc gacttcgagc gcgagcgcct tcgtgaagcc
    14821 gtgcatgccg gccttcgccg ccgagtagtt cgtctgcccg aacgcgccct tctgcccgtt
    14881 gaccgacgag atgttgatga tgcgccccca gccgcgctcc gtcatgccgt cgcatacctg
    14941 cttcgtcatg ttgaagcacg aatcgagatt cgtcccaatc accgcatccc agttcgcctt
    15001 gtccatcttg cgaaacgtca tgtcgcgcgt gatgccggcg ttgttcacga gaacgtcgat
    15061 ggggccgagt tcgttcgtca cctgcctgac gcatgcggag gccgaatcga agtgcgcgac
    15121 gtcacatgca taggcgcgaa aatcgtaacc ctggcgcttc atcgcctcga gccagtcggc
    15181 cgacttcgtg ttgcccggcg aatgcgtggt gacgaccgta ttgcccagcg ccgagagctt
    15241 ggtgcagatt gcttcgccga gaccgcccat gccaccggtg acgagcgcaa tgcgctgtgc
    15301 tgccatgatc gtttcctcct gcgacgccga tgccggcatg gtagcgcaac ggcgggcagc
    15361 gtgacgaacg cgctctgttg cagtgcgaca cgacgcgcag tgcggccgct gttgccgttt
    15421 gtgttcggcc ggactagagt gccgcttgcg ataggcagcg ggagccggtc ctgcatcgtg
    15481 cgatgcgggc ttgtcatcaa cgggaggggt cgatgaaatt ttcgatgaaa gctgttgccg
    15541 tgttgtgcgc gcttgcaatc tgcccgatcg cggggcaggc cgacgagctc gaaacgctga
    15601 gcgggatgca ccagacgaac acgccggtcg actggccgat gattccgcag accggcgcga
    15661 aggccgacca gatcaagcag aacctcacca agatcaagct gccgcccggc ttccatatcg
    15721 ggctctacgc gatcgttccc gacgcccggc atatcgcggt gggtccgcag ggaatcgtca
    15781 cgttcgtcgg cacgcgcaag accaaggtgt gggcggtgac cgatcgctcg cgcggcggtg
    15841 tcgccgacga ggtgaaggaa ttcgcgccga cgctaccgaa gaggattccg aacggtccgt
    15901 gcttctcgaa ggacggattc ctctacgtcg tcgagcagaa tcgcgtcgtc gaatatccgg
    15961 cggccgaatt cttctacgaa agccccgatg tcgtgcccaa cgcgatcgtt cccgaaggga
    16021 agctgattcc caaggaggag gaaagctaca accatacggc gcgtccctgc cgcatcggcc
    16081 ccgacaacaa gctctatatc cagctcggcc agccgtacaa cgtgccgccg aaggagaagc
    16141 tcgacctgta tcacaagacc gggatgatgg gcatcatccg gatggatcgc gatggcaaga
    16201 accgcgaggt ttatgccacc gggttgcgca acgcggtcgg gatggatttc aacccgaagg
    16261 acaagacgct gtggagcaac gacaaccagg tcgacggcat gggcgacgat cagccgcccg
    16321 gtgagttgaa ccgaatcgcg aagcaggggc tcgacttcgg cttcccctgg tacggcggcg
    16381 gacacacgcg cacgaccgaa tacaagaacg acacgccgcc cgccggggtc gtgttccccg
    16441 aggtggagca gatagcgcac gcggcggacc tcggtctcct gttctacacc ggcacgatgt
    16501 ttcccgcgaa gtaccgtgga gcgattttct cgacgcagca cggctcgtgg aaccgcacgg
    16561 tgcccgccgg cgcacgcgtg ctcgtgacgt tcgtcaagga agacggccat gtcgccggca
    16621 agtcggagcc gttcgccgaa ggatggaacg acaacggcta ttacctgggg cgtccggtcg
    16681 acgtcgcgga actgcacgac ggctcgctcc tcgtgtccga cgatctcgtc ggcgcgctgt
    16741 atcgcatctg gtacgacggc aaatgatgcg ggcatcggcg gcgctcgtgg gcgccgccgc
    16801 ccttctttgc ttcgcggccc cggaggcgcg cgccgccggt gacgcgaagg ccggacgcat
    16861 gaaggcgcaa atgtgccagg catgccacgg caccgacggg ttgtcgcagg ttcccgacgc
    16921 gccgaacatc gcgggccagg tcgaaggcta cctcgtggcc cagttgcagg cgttcaaatc
    16981 gggcgcgcgg aagaacgagg ccatgacgct cgtcgcggca gggctgtcga gccaggacat
    17041 cgacaatctc gccgcgtggt attcgtcgat cgagatcaag ctcggcaagc tgccgggcgg
    17101 gtgacgcgcg cttcagctca cgcgctcgac gacgtattcg ccgggcgctc gggcgagcgg
    17161 ccgatgggtt gcgttcccgg tgcgcttcgg ggctgcgcgc tcgccgcccc tgtgctgcgc
    17221 aagccagcga taccagtgcg gccaccagct tccggtatgg ctcgtcgcat tcgccaccca
    17281 tgcgtccgtc gagcgctcgc gtccatcgtg cgtccagtaa ttgcgcttgt gcgcttcggg
    17341 cgcattgacg acgcccgcga tgtgccccga ggcgccgagc acgaaggtgg tttcgccgcc
    17401 gacgaggtcg agcgtgcgaa acgccgagcg ccagggaacg atatggtcgt cgcgcgacgc
    17461 atagacgtac gcgggcaatg cgacgcgcga tagatcgatc ttctcgccca gcatcgtcaa
    17521 tgcgccgcgt tcgcgcagcc ggttctcgag atacagctgg cggatgtaat acgcgtacat
    17581 cggcccggga aggttcgcgg aatcgccgtt ccagtacagg aggtcgaacg ccggcggctt
    17641 tctgcccttc agatagttgt cgacgacgta gttccaaacg agatcgttcg ggcgcaggct
    17701 ggcgaatgcg ccggcgagct cgcttccatg cacgcgctgg ccggcggcga gctgcgcttc
    17761 gcgcgccgcc agcatctgcc ggctgacgta gacgccgatt tcgccggggt cggcgaagtc
    17821 gagcatcgtc gtcaggaacg tgacgctcga tgcactcgtg tcgttgcgcg ccgcgagcac
    17881 ggcgagtgcg cacgcgagga gcgtgccgcc gacgcagaag ccgagcgtat tcacgtcgcg
    17941 cgatcggctg atcgccttga ccgccgtcag tgccgccaga acgccttgcg ccaggtagtc
    18001 gtcccagctc agcgtgccga gctcggcagg cacgttgcgc cacgagatca tgaacacggt
    18061 atggccctgc tcgacggcat ggcgaacgaa cgaattcgcc ggcttcagat cgaggatgta
    18121 gtacttgttg atgcaaggcg gcacgatcag cagcggccgg cgcgcgacgc gtggcgtcgt
    18181 tgcgtcgtac tggatcagtt cgatcagatc gttgcggagc acgacgctgc ccggcgtcac
    18241 cgcgaggttg cggccgacct cgaaggccga ctcgtcgctc atcgtgatgc ggcccttcgc
    18301 gacatcggcg gccaggttgc gcaagccatc gacgacgctc gcaccttcgg tctcgatggc
    18361 gcgcgcgagc acgtcggggt tggtcgcggg gaaattcgtc ggtgcgattg cgtcgacgaa
    18421 ctggcgcacc gagaactcga gccgcttctt ttcgtcgggc ggcagctgtg caatcgatgc
    18481 gagtgcggtc aggaactcgc cgtaaagccg gtacgactgc gcaagccacg cgaaataggg
    18541 ccgctcgcgc cacgcggccg aggcaaagcg gcgatcgtcg gcgggtgcac gcgcctgcgc
    18601 cggcgccgcc cacagcgcct gcacgcgcgg cgcgaacgcc gcgtgcaatg cggcaacgcc
    18661 ttcggcgtcg aacggcgcac gaatcgtggt tgcgaccgcg gacgcttcgg cacccggcac
    18721 ggttcgcccg gcgccggccc accacgccgt ccacgattgc agcgcctgct gccattgcgc
    18781 ggcgagcgca agccacgatt cgatcgtatg tggttcagga acagccatgg gtgcaaaggg
    18841 cggaccgttg accgatgtta tcccggctcg agcagcagat gcgcaacggc gatgattgca
    18901 ataccgaggc cgatccagaa catctgcttc g
//
LOCUS       DASSVX010000139        10072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_988625, whole genome shotgun sequence.
ACCESSION   DASSVX010000139 DASSVX010000000
VERSION     DASSVX010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 10072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10072
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988625"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="VFO53_06115"
     CDS             complement(<1..1129)
                     /locus_tag="VFO53_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET8875888.1"
                     /translation="MQFPLNDAQRDAVRYDSAPLLVLAGAGSGKTRVITAKIAHLVGR
                     GLEPSRIVAITFTNKAAREMRERAEQALRGAGVASRLSISTFHSLGLRIVREEAAALG
                     LKRGFSIFDPGDIEAIVAELAATADRARTRAIQWKISQWKNALVDPPAALNAASDDAE
                     LAAARAYAGYRDALLAYQAVDFDDLIALPVTLLDNDGAARARWQSRCAHLLIDEYQDT
                     NAAQYRLFRHLVGAETPFTAVGDDDQAIYGWRGATLDNLAALPRDFPALRVIKLEQNY
                     RSTVRILRCANALIGNNPKLFDKRLWTELAHGEALRVAPAADEEAEADLVIRRILAHK
                     FEHRTKLADYAILYRGNHQARPFETALRAQSIPYEISGGQSM"
     gene            1230..2057
                     /locus_tag="VFO53_06120"
     CDS             1230..2057
                     /locus_tag="VFO53_06120"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HET8875889.1"
                     /translation="MAHYAIGDIQGCHAELCELLALIGFSPATDRLWLTGDLVNRGPD
                     SLGVLREVRALGDAAQTVLGNHDLHLLTVAAGHRLSHRDDTIASILAAADRDELLEWL
                     VRQPLAIAEGNLLMVHAGVLPQWSTARVLALAGEVEAVLASAGREPFLRVLYGNEPHA
                     WRDDLEGFDRLRAIVNALTRLRFCTSDGRMDFSEKRGAVHAPPGFAPWFLHEQRRSAS
                     SLVLCGHWSTLGLTLAPNVLMLDSGCLWGGPLTAIRLSDRRIYQVPSHAQVTPVPRE"
     gene            complement(1978..2763)
                     /locus_tag="VFO53_06125"
     CDS             complement(1978..2763)
                     /locus_tag="VFO53_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET8875890.1"
                     /translation="MTAALRRLARIPRVMLHFVIGLATVLFAFPSLDAKARRVRIRRW
                     SRRLLHLMSVEIRINGALAHPNVMVVANHVSWLDIFALHAVGPVRFIAKSEIARWPLI
                     GRLVRGVGTLFIERARRHDTHRVNREVAAALAEGDIVAVFPEGTVTDGTRLLPFKGSL
                     LQPIIESGGCVQPVAIRYRTRTGDLSRAPAYDDVTFLHSFWRICGERAIVADIVATPA
                     LPATARHRRALAADAEAVIRAVLASPAHDSAPGRCADRTGGSP"
     gene            complement(2780..3715)
                     /locus_tag="VFO53_06130"
     CDS             complement(2780..3715)
                     /locus_tag="VFO53_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET8875891.1"
                     /translation="MRTLICGSLAFDTIMVFPDRFARHILPEQTHMLSVAFQISDMRR
                     EWGGCAGNIAYNLRGIGGDPVVMGTLGSDGTAYRGRMQDLGIATDGVRIIDDAYTAQA
                     FIITDLDDNQITAFHPGAMNLSHRNSVGDVPDIGIGIVAPDGKEGMQAHAAQFAARGV
                     PFLFDPGQGLPLFSGEELARLIEAANYLAVNDYEAKLLSERTGWSLGELAERVDALVV
                     TLGAEGSIIHADGATYAIAAAPASAVVDPTGCGDAYRAGLLYGIAAGWDWQRTGQLAS
                     AMGSIKIACRGGQNHRVDRELVTQCYFDAFGERPW"
     gene            complement(3892..4674)
                     /locus_tag="VFO53_06135"
     CDS             complement(3892..4674)
                     /locus_tag="VFO53_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HET8875892.1"
                     /translation="MPEAPLRILLVDDEAPARHRLRELLDDCAAVLPISIVGEAQHGR
                     EALDILHSASADLVLTDIHMPAMDGIELARHLLKLSHPPVVIFTTAFHQHALAAFEVH
                     AVDYLVKPVRVQRLLAALQKVPRLKPMTVEKLTQLPAAARRFLSVTERSRVVLVPIDE
                     VVYLKAELKYITIRTAQREYLLEESLTRLEQEFGARFVRVHRNCLVARDFIRGFERRA
                     NDDGDAHWEVLLHGIAETLPVSRRQQYIVREIGREVMNGAAA"
     gene            complement(4667..5779)
                     /locus_tag="VFO53_06140"
     CDS             complement(4667..5779)
                     /locus_tag="VFO53_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET8875893.1"
                     /translation="MPDLRNLGTVLRILLGVNGAALLIALAREPRLPAVATEWATLTA
                     FVEPQLFAELAVLWLVQPWLARLDGRAAIGVVTVITCALALGVDALFPRMAGEARGSL
                     VRGLVFALTAQFVLIAYFRLRARALSPAITEARLQALQARIRPHFLFNSITAVLSLMR
                     SNPRRAEGALEDLADLFRVLMRDNRELAPLADEVELCRQYLELEQLRLGERLVIDWNV
                     KSMPGDALVPPLVLQPLLENAVYHGIEPSTVPGVVSINIFLSRDEVHALLRNPYTANG
                     ARHHAGNRMALDNIRERLALHFDAEASLESRVNRDTYEVHIRMPYRTTKTAAAGSGTQ
                     ATARREGSAGQDTAKRREPYLRQRVPALAQPRIDHA"
     gene            5982..7436
                     /gene="argH"
                     /locus_tag="VFO53_06145"
     CDS             5982..7436
                     /gene="argH"
                     /locus_tag="VFO53_06145"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET8875894.1"
                     /translation="MSAPEPSSDPDSAKPPNAATPVPPSGTWSGRFSEPMSERMQRFN
                     ASVDFDRRLARVDIAGSRAHAQMLAERGIITHDDAQAIERGLTQIEGEIARGEFAWER
                     ALEDVHFNIERRLTALTGDAGKRLHTARSRNDQVATDIRLWLREAIDALVSQLVALRR
                     ALHDLATRHADTVMPGFTHLQVAQPVTFGHHLMAYDAMLARDVERFADCRKRVNRLPL
                     GSAALAGTSYPIDRERVARALGFDSVTTNSLDAVSDRDFAIEFEAAAALVMVHLSRFA
                     EELVLWSSPRFGFVTLADRFCTGSSIMPQKKNPDVPELVRGKSARVIGHLTALLVLMK
                     AQPLAYNKDNQEDKEPLFDTVDTLADVLAIVTDLVATGLTANVARMREAAREGHATAT
                     DLADYLVRKGVAFRDAHEAVARAVREAEARQCDLAALPLDALQRFSTLIDADVFGVLT
                     LDGSVASRDHIGGTAPSRVRAAIDAARREIGSST"
     gene            7379..8299
                     /locus_tag="VFO53_06150"
     CDS             7379..8299
                     /locus_tag="VFO53_06150"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8875895.1"
                     /translation="MTGSRGNRRRATRNRELDMNKLAFLGLGVMGYPMAGHLAKAGHD
                     VTVYNRTPAKAAQWVAQYGGRSMPSPADAARDAQIVLMCVGNDNDVRAVAKEALAGMS
                     SEAMLVDHTTASAEVAREIAAAAKARNVAFLDAPVSGGQAGAENGKLTIMVGGDPAVF
                     AKAQPVLALYGRAVTLMGATGAGQLTKMVNQICIAGLVQALSEGISFAQRAGLDPNLV
                     LDVISKGAAQSWQMENRGRTMVDDKFDFGFAVDWMRKDLGICLAAARGNGAALPVTAL
                     VDQFYARVQARGGGRWDTSSLIRLLRDERS"
     gene            complement(8304..8750)
                     /locus_tag="VFO53_06155"
     CDS             complement(8304..8750)
                     /locus_tag="VFO53_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET8875896.1"
                     /translation="MKARVKWVEQVSFVAEAGSGHAVVVDGAPEAGGRNLGMRPMELV
                     LAGAAACTAFDVVWILKRARQPVADCVVDAEAERAAQEPKVFTHIHLKYAIAGRGLDA
                     RQVERAVKLSKEKYCSATIMLAHSASISYEVRIVEGDDVGEAGPDP"
     gene            9159..9587
                     /gene="rplM"
                     /locus_tag="VFO53_06160"
     CDS             9159..9587
                     /gene="rplM"
                     /locus_tag="VFO53_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET8875897.1"
                     /translation="MKTYSARPRDVTRAWFVVDASDKVLGRLATQIAARLRGKHKAIY
                     TPHIDTGDFIVVTNVDKIRVTGTKADSKLYHRHSGYPGGITTTTFGQMQARFPGRALE
                     KAVKGMLPKGPLGYAMIKKLKCYAGATHPHSAQQPTALDI"
     gene            9591..9983
                     /gene="rpsI"
                     /locus_tag="VFO53_06165"
     CDS             9591..9983
                     /gene="rpsI"
                     /locus_tag="VFO53_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET8875898.1"
                     /translation="MINNYHYGTGRRKTSVARVFMKRGDGKIVVNDKPVDEFFTRETG
                     RMVVRQPLVLTNHQGTFDIMVNVIGGGESGQAGAVRHGITRALIDYDAELKPALSKAG
                     LVTRDAREVERKKVGLHKARRRKQFSKR"
BASE COUNT         1685 a         3399 c         3393 g         1595 t
ORIGIN      
        1 acatcgattg cccgccggag atttcgtacg ggatcgactg cgcgcgcagc gccgtttcga
       61 acggacgcgc ctggtgattg ccgcgataga ggatcgcgta atcggcaagc ttcgtgcggt
      121 gctcgaactt gtgcgcgagg atgcggcgga tgacgagatc ggcctccgcc tcttcgtccg
      181 cggcgggtgc cacgcgcagc gcctcgccgt gcgcgagctc ggtccacagc cgcttgtcga
      241 aaagcttcgg attgttgccg atcagcgcgt tcgcgcaacg gaggatgcgg acggtcgagc
      301 ggtagttctg ctcgagcttg atgacgcgaa gcgcaggaaa atcgcgcggc agcgctgcga
      361 gattgtcgag cgtggcgccg cgccagccgt agatcgcctg gtcgtcgtcg ccgacggccg
      421 tgaacggcgt ttcggctccc accagatggc ggaacagccg gtattgcgcg gcattcgtgt
      481 cctggtattc gtcgatgagc agatgcgcgc agcgcgactg ccagcgcgcg cgcgccgcgc
      541 catcgttgtc gagcagcgtg accggcagcg cgatcagatc gtcgaagtcc accgcctgat
      601 acgcgagcaa tgcgtcgcgg taaccggcat aggcgcgtgc ggccgcaagc tcggcgtcgt
      661 cacttgccgc gttcaacgcc gccgggggat cgacgagtgc atttttccac tggctgatct
      721 tccactggat ggcgcgggtg cgcgcccgat cggccgtcgc ggcgagctcg gcgacgatcg
      781 cttcgatgtc gccgggatcg aagatcgaaa agccgcgctt caatcccagc gccgcggctt
      841 cctcgcgcac gatgcgcagg ccgaggctgt gaaacgtcga gatcgacagc ctggacgcga
      901 cgccggcgcc gcgaagcgcc tgctcggcac gctcgcgcat ttcgcgcgcc gccttgttcg
      961 tgaacgtgat cgcgacgatg cgcgacggct cgaggccgcg accgacgaga tgcgcgatct
     1021 tcgcggtgat gacgcgcgtc ttgccactgc ccgcgccggc gagcacgagc agcggcgcgc
     1081 tgtcgtaacg caccgcgtcg cgctgcgcgt cgttcagggg gaactgcaag ggagcgaatg
     1141 cgatgcgttg ctacaatggc ccgattctac cgacgcgatg cgaagagcgg cagtcttgac
     1201 gcgcgcgcga caacccactc tcgaattcca tggcgcacta cgcgatcggt gatatccagg
     1261 ggtgccatgc ggaattgtgc gaactgctcg cgctgatcgg cttttcgccg gcaaccgatc
     1321 ggctctggct cacgggcgat ctcgtcaatc gcgggcccga ttccctcggc gtgctgcgcg
     1381 aggtgcgcgc gctcggcgat gcggcgcaaa cggtgctcgg caatcacgac ctgcatttgc
     1441 tcaccgtcgc cgccggtcac cggctgtcgc atcgcgacga cacgatcgca tcgattcttg
     1501 ccgcggccga tcgcgacgaa ttgctcgaat ggctcgtgcg ccagccgctc gccatcgcgg
     1561 aaggcaacct gctgatggtg cacgcgggcg tgctgccgca atggagtacg gcgcgggtgc
     1621 tcgcgctggc gggggaggtc gaggccgtcc tcgcgagcgc cgggcgcgag cccttcctgc
     1681 gtgtgttgta cggcaacgag ccgcacgcgt ggcgcgatga cctcgaaggt ttcgaccggt
     1741 tgcgtgcgat cgtcaacgcg ctcacgcgct tgcgcttttg cacgagcgat ggacgcatgg
     1801 actttagcga aaagcgcggc gcggtccacg caccgcccgg cttcgcaccg tggtttctcc
     1861 acgagcagcg gcgcagcgcg tcgtcgctcg tgctctgcgg tcactggtcc acgctcgggc
     1921 tgacactcgc cccgaacgtg ctgatgctcg attccgggtg cctgtggggc ggcccgctca
     1981 cggcgatccg cctgtccgat cggcgcatct accaggtgcc gagtcatgcg caggtgacgc
     2041 cagtaccgcg cgaatgaccg cctcggcgtc ggccgccagc gcgcggcgat gcctggccgt
     2101 ggcgggcagc gccggcgtcg ccacgatatc ggcgacgatc gcgcgctcgc cgcagatccg
     2161 ccagaacgag tgcaggaacg tcacgtcgtc gtacgccggt gcgcgcgaga ggtcgccggt
     2221 gcgcgtgcga tagcggatcg caaccggctg cacgcacccg cccgattcga tgatcggctg
     2281 cagcagcgat cccttgaatg gcaggagcct ggtcccgtcg gtgacggtcc cctcgggaaa
     2341 gaccgcgacg atgtcgcctt cggcgagcgc cgcggcgacc tcgcggttga cccgatgcgt
     2401 gtcgtgccgg cgtgcgcgtt cgatgaacag cgtgccaacg ccgcgcacga gccgtccgat
     2461 cagcggccag cgcgcgatct ccgacttggc gatgaagcgg accggaccga cagcgtgcag
     2521 cgcgaagatg tcgagccacg acacgtgatt ggcgaccacc atcacgttcg ggtgcgcgag
     2581 cgccccgttg atgcggatct cgacgctcat caggtgcagc agccgccgcg accagcggcg
     2641 gatgcgcacg cggcgcgcct tcgcatcgag cgacggaaaa gcgaacagca ccgtggcgag
     2701 tccgatcacg aaatgcagca tgacgcgcgg aatgcgcgcc agcctgcgca gcgccgcggt
     2761 caagggcgca ggccgccgtt caccacggcc gttcgccgaa cgcgtcgaaa tagcactgcg
     2821 tcacgagctc gcggtcgacc cgatgattct ggccgccgcg gcaggcgatc ttgatcgatc
     2881 ccatcgccga cgcaagctga ccggtgcgct gccagtccca cccggcggca atcccgtaga
     2941 gcagccccgc acggtacgcg tcgccgcatc cggtgggatc gacgacggca gacgccgggg
     3001 cggcggcaat cgcgtacgtc gcgccatcgg catggatgat cgacccttcg gcgccaagcg
     3061 tcacgaccag cgcatcgacg cgctcggcga gctcgccgag cgaccagcct gtacgctccg
     3121 aaagcagctt cgcctcgtaa tcgttgacgg ccagatagtt cgccgcttcg atgagtcgcg
     3181 cgagctcctc gcccgagaaa agcggcaacc cctggccggg gtcgaacagg aacggcacgc
     3241 cgcgcgcggc gaactgcgcg gcgtgggcct gcatgccctc cttgccgtcc ggcgcgacga
     3301 tgccgatgcc gatgtccggt acgtcgccga cactgttgcg atgcgacagg ttcatcgcgc
     3361 cgggatgaaa cgccgtgatc tggttgtcgt cgaggtcggt gatgatgaat gcctgcgccg
     3421 tgtacgcgtc gtcgatgata cgaacgccat ccgtcgcgat gccgaggtcc tgcatgcggc
     3481 cgcgatacgc ggtaccgtcg ctgccgagcg tgcccatgac gaccggatcg ccgccgatgc
     3541 cacgcaggtt gtacgcaatg ttgcccgcgc agccgcccca ttcacggcgc atgtcgctga
     3601 tctggaaggc caccgagagc atgtgcgtct gctcgggcag gatgtggcgc gcgaagcgat
     3661 cgggaaacac catgatcgtg tcgaacgcga gcgagccgca gatcagtgtg cgcatgggca
     3721 ggccgagcgg gagcgaaggg gagacccgcc ggggcgggcc gcgccgaagt gtagcgcaac
     3781 agggtcagag acgattttcc tgcgcgccgg cgatcggcgt aactgccatg ctcaccgaaa
     3841 atcgtctctg accctgtttc ggtgttggcg gccggaaaat cgtctctgcc cctacgccgc
     3901 cgcgccgttc atcacctcgc ggccgatctc gcgcacgatg tattgctggc ggcgcgacac
     3961 gggcagcgtt tcggcgatgc cgtgcagcag cacttcccag tgcgcgtcac cgtcgtcgtt
     4021 ggcgcggcgc tcgaagccgc gaatgaaatc gcgcgcgacg aggcagttgc gatgcacgcg
     4081 cacgaagcgc gcgccgaatt cctgctcgag ccgagtcagc gattcctcga gcagatactc
     4141 gcgttgcgcg gtgcggatcg tgatgtactt gagctcggcc ttgaggtaga cgacctcgtc
     4201 gatcggcacg agcaccacgc gcgagcgctc ggtcaccgac aggaagcgcc gcgcggccgc
     4261 cggcagctgc gtgagcttct cgaccgtcat cggcttgagg cgcggcacct tttgcagcgc
     4321 ggccagcaac cgctgcacgc gtaccggctt gacgaggtaa tcgaccgcgt gcacttcgaa
     4381 tgccgccagc gcgtgctggt ggaatgccgt cgtgaagatg accaccggcg gatgcgaaag
     4441 cttgagcagg tgccgcgcca gctcgatgcc atccatcgcc ggcatgtgaa tgtcggtcag
     4501 cacgaggtcg gccgaggccg aatgcagaat gtcgagcgcc tcgcgcccat gctgcgcctc
     4561 gccgacgatc gagatcggaa gcacggccgc gcaatcgtcg aggagctcgc gcaagcgatg
     4621 tcgcgcgggc gcctcgtcgt cgacgagcag gatgcggagc ggtgcttcag gcatggtcga
     4681 tcctcggttg cgccagcgcc ggcacgcgtt gtcgcaggta gggctcgcgg cgcttcgcgg
     4741 tgtcctggcc ggcgctcccc tcgcggcgcg ccgttgcctg cgtgccgctg cccgcggcgg
     4801 cggtcttcgt ggtgcgatag ggcatcctga tgtgcacctc gtacgtgtcg cggttgaccc
     4861 gcgactcgag gctcgcctcg gcgtcgaaat gcagcgcgag ccgctcgcgg atgttgtcga
     4921 gcgccatccg gttaccggca tgatgccgcg cgccgttggc ggtgtacggg ttgcgcagca
     4981 gcgcgtgcac ttcgtcgcgc gacaggaaga tgttgatcga caccacgccg ggaacggtcg
     5041 acggctcgat gccatggtag accgcattct cgagcagcgg ctgcagcacg agtggcggca
     5101 ccagcgcgtc gcccggcatg ctcttcacgt tccagtcgat gacgagccgt tcgccgagac
     5161 gcagctgctc gagctcgaga tactgccggc aaagctcgac ctcgtcggcg agcggggcaa
     5221 gctcgcggtt gtcgcgcatc agcacgcgaa acagatcggc gagatcctcg agcgcaccct
     5281 cggcgcgcct cgggttgctg cgcatcagcg acagcaccgc ggtgatgctg ttgaacagga
     5341 aatgcggccg gatgcgcgcc tgcagcgcct gcaggcgcgc ttcggtgatt gccggcgaca
     5401 gtgcgcgggc gcgcaggcgg aagtaggcga tcaacacgaa ttgcgcggtc aacgcgaaca
     5461 cgaggccgcg cacgagcgat ccgcgcgcct cgccggccat ccgcggaaag agcgcgtcga
     5521 cgccgagcgc gagcgcacac gtgatcacgg tgacgacacc gatcgccgcg cggccgtcga
     5581 gtcgcgcgag ccagggctgc acgagccaga gaacggccag ctcggcaaaa agttgcggct
     5641 cgacgaacgc ggtcagcgtg gcccattcgg ttgcaaccgc gggcaggcgc ggctcacgcg
     5701 cgagcgcgat caggagcgcc gcgccattga cgccgagcag gatgcgcagg accgtcccga
     5761 gattgcgcag atcgggcaat gcgggtacgg ccggcggttg ttttataatc ccctgcataa
     5821 cggacgcgat atgcaagcat catgccggat gccgccattc gggcccggcg cggatagcat
     5881 aacggctcgc cgaaacgcgt cgctcccgcg cgggtcatgt gggtcgcggg ccacgcctcg
     5941 ccattgtcga agcgcgaagc gcggagtgta gttcaacatc catgtccgcc cccgaaccgt
     6001 cgtccgatcc cgacagcgcc aagccgccga acgccgccac tccggtgccg ccgagcggca
     6061 cctggtcggg ccgcttcagc gagccgatgt ccgaacggat gcagcggttc aatgcgtccg
     6121 tcgacttcga ccggcgcctg gcgcgcgtcg acatcgcggg ttcgcgtgcc cacgcgcaga
     6181 tgctcgccga acgcggcatc atcacccatg acgacgcgca ggcgatcgag cgcggactca
     6241 cgcagatcga gggcgaaatc gcgcgcggtg aattcgcgtg ggagcgcgcg ctcgaggacg
     6301 tgcatttcaa catcgagcgg cggctcaccg cgctgacagg cgacgccggc aagcggctgc
     6361 acacggcgcg ctcgcgcaac gaccaggtcg caaccgacat ccgcctgtgg ctgcgcgaag
     6421 cgatcgacgc gctcgtttcg caactcgtcg cgctgcgccg cgcgctgcat gacctcgcca
     6481 cgcgccacgc agataccgtc atgccggggt tcacgcatct gcaggtcgcg cagccggtga
     6541 cgttcggcca tcacctgatg gcgtacgacg cgatgctcgc gcgcgacgtc gagcgcttcg
     6601 ccgactgcag gaagcgcgtc aaccgcctgc cgctcggcag cgcggcgctt gccggaacct
     6661 cttatccgat cgaccgcgag cgcgttgcgc gcgcgctcgg cttcgactcg gtgacgacga
     6721 actcgctcga tgcggtttcc gatcgcgatt tcgcgatcga attcgaagcg gcggccgcgc
     6781 tcgtgatggt gcatctgtcg cgcttcgccg aggagctcgt gctgtggtca agcccgcgct
     6841 tcggcttcgt cacgctcgcc gaccgcttct gcacgggcag ctcgatcatg ccgcagaaga
     6901 aaaacccgga cgttcccgag ctcgtgcgcg gcaagtcggc acgcgtgatc ggtcatctga
     6961 cggcgctgct cgtgctgatg aaggcgcagc cgctcgccta caacaaggat aaccaggaag
     7021 acaaggagcc gctgttcgac accgtcgaca cgctcgcgga cgtgctcgcg atcgtcaccg
     7081 acctcgtcgc aaccgggctc acggcgaacg tcgcgcggat gcgcgaggcg gcgcgcgaag
     7141 gtcatgcgac tgcaacggat ctcgccgatt acctggtgcg aaagggcgtc gcctttcgcg
     7201 atgcccacga agcggttgcg cgtgcggtgc gcgaggccga agcgcgacag tgcgatctcg
     7261 ccgcactgcc gctcgacgcg ctgcaacgct tctcgaccct tatcgacgcc gacgtgttcg
     7321 gcgtgctcac gctcgacggc tcggtcgcga gccgcgatca catcggcggc acggcaccat
     7381 cacgggttcg cgcggcaatc gacgccgcgc gacgcgaaat cgggagctcg acatgaacaa
     7441 gcttgcattc ctcggcctcg gcgtcatggg ctatccgatg gccggccatc tcgcgaaggc
     7501 gggacacgac gtcaccgtgt acaaccgcac gccggcgaag gccgcgcaat gggtcgcgca
     7561 gtacgggggc agatcgatgc cgtcgccggc cgatgcggca cgcgatgcgc agatcgtgct
     7621 gatgtgcgtg ggcaacgaca acgacgtgcg cgccgtcgcc aaggaggcgc tcgccggcat
     7681 gagcagcgaa gccatgctcg tcgatcacac cacggcgtcg gccgaagtcg cgcgcgagat
     7741 tgcggccgcc gcgaaggcgc gcaacgtggc cttcctcgat gcgccggtat cgggtgggca
     7801 ggccggcgcc gagaacggca agctcacgat catggtcggc ggcgatcccg cggtgttcgc
     7861 gaaggcgcag ccggtgctcg cgctttacgg acgcgccgtg acgctgatgg gtgcaaccgg
     7921 cgcgggccag ctcaccaaga tggtcaacca gatctgcatc gcaggtctcg tgcaggcgct
     7981 gtccgaaggc atcagcttcg cgcagcgcgc gggactcgac ccgaacctcg ttctcgacgt
     8041 gatcagcaag ggcgccgcac agtcgtggca aatggagaat cgcggccgga cgatggtcga
     8101 cgacaagttc gacttcggtt tcgccgtcga ctggatgcgc aaggacctcg gcatctgcct
     8161 cgcggcggca cgcggcaacg gtgcggcgct gccggtcacg gccctcgtcg accagttcta
     8221 cgcgcgggtg caggcgcgcg gcggcggccg gtgggacacg tcgagcctga ttcgcctgct
     8281 gagggacgag cgatcgtgac ggactagggg tccgggccgg cttccccgac atcgtccccc
     8341 tcgacgatgc gcacctcgta gctgatgctg gccgagtgcg ccagcatgat cgtcgccgag
     8401 cagtatttct ccttcgacag cttcaccgca cgctcgacct gcctcgcgtc aaggccgcgg
     8461 ccggcgatcg catatttgag gtgaatatgc gtgaagacct tcggttcctg cgccgcgcgc
     8521 tcggcctcgg cgtcgacgac gcaatcggcc accggttggc gcgcgcgctt caggatccac
     8581 acgacgtcga acgccgtgca cgccgcggcg cccgcaagga cgagctccat cggccgcatg
     8641 ccgaggttgc gcccgccggc ctcgggcgcg ccgtcgacga cgaccgcgtg gccgctgccc
     8701 gcctcggcga cgaacgacac ctgctccacc catttgaccc gcgccttcat ctgtcgcgcc
     8761 cccgcgtccg gctgttgcgc cggtgcctgc cgccgatcgt acaaaccgtg cccttcgtct
     8821 tatgaccgct tatgcatctt gcattgtgca gcgcaatatg gaatgcttac gcctgtcgcg
     8881 ggttgccgtc tcctccttcg gcgcccgcgg cgcattcctc cattctcctc ctatggtgga
     8941 atactcggcg cggtcggtac gaccgcgcct tttttttcta ccgcggcgca ccatcgacgg
     9001 gccgatcccc actcgccgcc gctgccgaag cgcgtctcga gcctcgcgaa acatcgcgtt
     9061 gacccgcgcg acgaaagcat agtacaatca ccgactttgc tgaattgcca ccacaatctc
     9121 gcaatgcagc gcgtggtgga atcgtcggga aatttccaat gaagacctac tcggcccgtc
     9181 cgcgcgatgt cacgcgcgcc tggttcgtcg tcgacgcgtc agacaaggtg ctcggacggc
     9241 ttgccaccca gatcgccgcg cgccttcgcg gcaagcacaa ggccatctat acgccgcata
     9301 tcgacacggg cgacttcatc gtcgtcacca acgtcgacaa gatccgcgtc accggcacca
     9361 aggccgacag caagctctat caccggcata gcggctatcc gggcggcatc acgaccacca
     9421 cgttcggcca gatgcaggcc cgctttcccg gccgcgcact ggaaaaggcg gtcaagggca
     9481 tgctgccgaa ggggccgctc ggctacgcga tgatcaagaa gctcaagtgc tatgccggcg
     9541 ccacgcatcc gcattcggcg caacaaccga cagccctcga tatttagccc atgatcaaca
     9601 attatcatta cggcacggga cggcggaaga cctcggtcgc acgcgtgttc atgaagcgcg
     9661 gcgacggcaa gatcgtcgtc aacgacaagc ccgtcgacga attcttcacg cgcgagaccg
     9721 gacgcatggt cgtgcgccag ccgctggtgc tgaccaacca ccagggcacg ttcgacatca
     9781 tggtcaacgt gatcggcggc ggcgagtcgg gccaggcggg cgcggtgcgc cacggcatta
     9841 cccgcgcgct gatcgactac gacgcggagc tcaagccggc actgtcgaaa gcgggcctcg
     9901 tgacgcgcga cgcgcgcgaa gtcgagcgca agaaggtggg cctgcacaag gcgcggcgcc
     9961 gcaagcagtt ctcgaagcgc taaacgcttc cgaaaagtcc gcaaaaggcc gcctcttcag
    10021 gcggcctttt gccatattgg ggaatgagcg ggcggcgcga cgtcatatgc ca
//
LOCUS       DASSVX010000140         3968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_921912, whole genome shotgun sequence.
ACCESSION   DASSVX010000140 DASSVX010000000
VERSION     DASSVX010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921912"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VFO53_06170"
     CDS             complement(<1..1112)
                     /locus_tag="VFO53_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875899.1"
                     /translation="MPSSCLAGRSRRARLSSFVLVGCLFASALAPAQPIDRAADALLA
                     VDQQRPSVVERIVTTWGPALAKSRAPVSVDELRARLMSLRADRLFAASIAGTEEGLRE
                     VTGFPATTAATKPVAYAKALGDAAIDVVYTPVTPCRLVETRGTFAAVYQGNGSASHTP
                     VPFAPNEIRSYTVQGGNGVCLTQLPGGLSPSAVQLQVFGIPTTAASGDIEILPQGATF
                     GSTATMVYVASLNFNTVSTAAKINTSNKQIGVQVRGGGAHLAIDVVGYFAAPSGNGGR
                     FFRQGGNAFGTAAALGTIDNQPLALLANGQPAMTYLPNATSPNIIGGHASNSVDSSLS
                     GQTIAGGGFAGDNCFDSATNTSTRSCLNMTTGNDAS"
     gene            complement(1243..1773)
                     /locus_tag="VFO53_06175"
     CDS             complement(1243..1773)
                     /locus_tag="VFO53_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET8875900.1"
                     /translation="MIRDVLRMGDPRLFERSREVDRAGTPELLALLDDMRETMHAQNG
                     AGLAAPQIGVGLRVVIFGVERNERYPDAEPVPYTELVNPVLTPLSDELEDGWEGCLSV
                     PGLRGVVPRFRRLRYRGLDPSGAIIERDVEGFHARVVQHECDHLDGILYPMRVRDFTR
                     FGFTDVLFPGIAAAEE"
     gene            complement(1763..2569)
                     /locus_tag="VFO53_06180"
     CDS             complement(1763..2569)
                     /locus_tag="VFO53_06180"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HET8875901.1"
                     /translation="MLAIIGGTGLTRLSTLAVTHREVIRTPYGEPSAALIFGEVAGNP
                     AIFLARHGHGHTIPPHRVNYRANLWALKEQGASAILAIASVGAIRGCSPGELVLPHQL
                     IDYTSGRANTFYDGGDQQVVHTDFTHPYSQPLRTVCLAGAKDGRIALRDGGVYGAVSG
                     PRLETAAEIDRMDRDGATLVGMTGMPEAGLARELSVEYASVCVVVNHAAGRGDSVASV
                     SMESIANVLETGMDRVRALIEHVVPRVPLAARDVEVRREAVILGANADDS"
     gene            2742..3902
                     /locus_tag="VFO53_06185"
     CDS             2742..3902
                     /locus_tag="VFO53_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET8875902.1"
                     /translation="MYLDRLFKLMADKQASDLYISCGAPINIKVNGVVHPVSSQSMDV
                     ETVRRIAYDLMTKEQAREFENEREMNLSHLDRSVGNFRINIFRQRGSIALVIRYVRNN
                     VPPFEALNLPPVLLDLVMAKRGLVLVAGATGSGKSTSLAAMIDHRNNNRSGHILTIED
                     PIEYLFDHRQSMVNQREIGIDTHNYNMALQSALREAPDLIMIGEIRDKETMQAALLHT
                     LTGHLCLSTIHANNSYHALSRIINLFPHEARSAVLSDLSIGLRAIVSQRLVRNVEGGQ
                     QPAVEILLNTSLIAELIKSGEFTQIKEAMEQSLYPGSQTFEQALCKLYLDGRISYDEA
                     MVASDSATNLAWLINQNSPSSRIDSAQGEPELNVRPAADFASMTINPELLER"
BASE COUNT          709 a         1357 c         1279 g          623 t
ORIGIN      
        1 atcgatgcat cattgcccgt tgtcatattg agacacgacc gcgtgctcgt gttcgtggcc
       61 gagtcgaagc aattgtcgcc ggcgaagccg ccgcccgcga tcgtctggcc cgataacgat
      121 gaatcgacgc tgttgctcgc atgcccgccg atgatgttcg ggctcgtcgc attcggcaga
      181 taagtcatcg caggctgacc gttcgcgagc aacgccaatg gctggttgtc gatcgtcccc
      241 agcgccgcgg ccgtcccgaa cgcgttgccg ccctggcgaa agaaccgtcc accgttgcca
      301 ctcggcgcgg caaagtaacc gacgacgtcg atcgccaggt gcgcgccgcc gccgcgtacc
      361 tgcacgccga tctgtttgtt cgacgtgttg atcttcgccg ccgtcgacac cgtgttgaag
      421 ttgagcgacg cgacgtacac catcgtcgcg gtgctgccga acgtcgcacc ctgcggcagg
      481 atctcgatgt cgcccgatgc agccgtcgtc ggaatgccga acacctgcaa ctgcacggcg
      541 ctcgggctca agccgcccgg cagttgcgtc aggcacacac cgttgccgcc ttgcaccgtg
      601 tagctgcgga tctcgttcgg cgcgaacggc accggcgtgt gcgacgcgct gccattgccc
      661 tgatagacgg cggcgaacgt gccgcgcgtc tcgacgagac ggcacggcgt caccggcgtg
      721 tagacgacgt cgatggcggc atcgccgagc gctttcgcgt aggcaacggg tttcgtcgcc
      781 gctgtcgttg ccgggaaacc cgtaacctcg cgcaagccct cctcggtgcc ggcaatgctt
      841 gccgcaaaga gccggtcggc gcgaaggctc atgagtcgcg cacgcaactc gtcgaccgaa
      901 acgggtgcgc gcgatttcgc gagcgccggt ccccaggtcg tcacgatgcg ctcgacgacg
      961 ctcgggcgct gctgatcgac ggcgagcagt gcgtcggctg cgcgatcgat cggctgtgca
     1021 ggcgcgagcg ccgaagcgaa caggcagcca acgaggacaa acgacgacag ccgcgcacgg
     1081 cgtgagcggc cggcaagaca cgatgacggc atggaagcct cccccggctt tccagcgcgg
     1141 catgcggccg cgcgtcacgt agcgcaaatg acgctacgcc gccagccaag ggcggtcaag
     1201 gcaatgcgga cagtcggtgc gccacggttt ccggcggctg gcttactcct ccgccgccgc
     1261 gattccggga aacagcacgt cggtgaaacc gaagcgcgtg aagtcccgca cccgcatcgg
     1321 atagaggatg ccgtccaggt gatcgcattc gtgctgcacg acgcgcgcgt gaaagccctc
     1381 gacgtcgcgc tcgatgatcg cgcccgaggg gtcgagaccg cgatagcgca gcctgcgaaa
     1441 gcgcggtacg acgccgcgca gccccggaac cgacaggcag ccctcccacc cgtcctcgag
     1501 ctcgtcggac aacggcgtca gcaccggatt gacgagctcc gtgtacggca ccggctcggc
     1561 gtcggggtag cgctcgttgc gctcgacacc gaaaatcacg acgcgcaggc cgaccccgat
     1621 ctggggtgct gcgagcccgg cgccattttg cgcatgcatc gtttcgcgca tgtcgtcgag
     1681 cagcgcgagc agttcgggcg tgccggcgcg atcgacttca cgcgagcgct cgaacaggcg
     1741 cgggtcaccc atgcgcagca catcacgaat catcggcgtt tgctcccagg atcaccgcct
     1801 cgcggcgcac ctcgacgtcg cgtgcggcga gcggcacccg cggcaccacg tgctcgatca
     1861 atgcgcgcac gcgatccatg cccgtttcga gcacgtttgc gatcgactcc atcgacacgc
     1921 tcgcgacgct gtcgccgcga cccgctgcgt gattgacgac gacgcacacc gacgcgtact
     1981 cgacgctgag ctcgcgcgcg agaccggcct ccggcatccc ggtcatgccg acgagcgtcg
     2041 cgccgtcgcg gtccatgcgg tcgatttccg ccgcggtttc gaggcgcggt cccgacacgg
     2101 cgccgtacac gccgccgtcg cgcagcgcga tgcggccatc tttcgcaccg gcgagacaca
     2161 ccgtgcgcag cggctgcgaa tacgggtgcg tgaaatcggt gtggaccacc tgctggtcgc
     2221 cgccgtcgta gaacgtgttc gcgcgtcccg acgtgtagtc gatcaactgg tgcggcagaa
     2281 cgagctcgcc ggggctgcag ccgcggatcg caccgaccga ggcgatcgcc aggatcgcgc
     2341 tcgcgccctg ctccttcaac gcccacaggt tcgcccggta gttgacgcgg tgcggcggaa
     2401 tcgtatgccc atgaccgtgg cgcgcgagaa agatcgccgg gttcccggcc acttcgccaa
     2461 agatgagcgc cgccgacggc tcaccgtagg gcgtgcgaat cacctcgcga tgcgtgacgg
     2521 cgagcgtgga aaggcgcgtg aggcctgtac cgccgatgat ggcgagcatg gaacttgctt
     2581 tgttgccggg tcgagttgtg gtgcgaagtc gagtggacgc ggtcggcagg cgacgcggaa
     2641 cgcccgcgcg ctggcggagc ccggcgactc atgacatcat catagccgca cgccccgggc
     2701 aggcaaggga ccgtgctgtc attctagccg gaaaggggac catgtatctc gaccgactct
     2761 tcaagctgat ggccgacaag caggcgtcgg atctctacat ttcctgcggc gcgccgatca
     2821 acatcaaggt caacggcgtc gtgcatccgg tgtcgagcca gtcgatggac gtcgagacgg
     2881 tgcgccgcat cgcgtacgac ctgatgacga aggagcaggc gcgggagttc gagaacgagc
     2941 gcgagatgaa cctgtcgcat ctcgaccgca gcgtcggcaa cttccggatc aacatcttcc
     3001 gccagcgcgg ctcgatcgcg ctcgtgatcc gctacgtgcg caacaacgtg ccgccgttcg
     3061 aggcgctgaa cctgccgccc gtgctcctcg acctcgtcat ggcgaagcgc ggcctcgtcc
     3121 tcgtcgccgg cgccaccggc tcgggcaagt cgacgtcgct ggccgcgatg atcgaccatc
     3181 ggaacaacaa ccgcagcggt cacatcctga cgatcgagga cccgatcgaa tacctgttcg
     3241 accaccgcca gagcatggtc aaccagcgcg agatcggcat cgatacgcac aactacaaca
     3301 tggcgctgca aagtgcgctg cgcgaagcgc ccgacctcat catgatcggc gagatccgcg
     3361 acaaggaaac gatgcaggcg gcgctgctgc atacgctgac cggccacctc tgcctgtcga
     3421 cgatccacgc gaacaacagc taccacgcac tgtcgcggat catcaacctg tttccgcacg
     3481 aggcacgctc cgcggtgctg tccgacctgt cgatcgggct gcgtgcgatt gtgtcgcagc
     3541 ggctggtgcg caacgtcgaa ggcggccagc agccggcggt cgagatcctt ctcaacacgt
     3601 cgctcatcgc cgagctcatc aagagcggcg agttcacgca gatcaaggaa gcgatggagc
     3661 agtcgctcta ccccggatcg cagacgttcg agcaggcgct atgcaagctc tatctggatg
     3721 ggcgcatctc ctacgacgag gcgatggtcg cgtccgactc ggcgaccaac ctcgcctggc
     3781 tcatcaacca gaactcgccg tcgtcgcgca tcgactccgc acagggcgag ccggagctga
     3841 acgtgcgccc ggcggcggat ttcgccagca tgacgatcaa ccccgaactg ctcgagcgct
     3901 gatcgatccg gcacccgcca cacgccgcac gccgcttgcg aaaatgacgc tcgcggttgc
     3961 ggcgaaac
//
LOCUS       DASSVX010000141         4123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1338553, whole genome shotgun sequence.
ACCESSION   DASSVX010000141 DASSVX010000000
VERSION     DASSVX010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338553"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFO53_06190"
     CDS             complement(<1..467)
                     /locus_tag="VFO53_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8875903.1"
                     /translation="MNVVRTDAELELPRVDRRLREYGATLVVLPETTSTVELAGAVRD
                     ADLLLMCYASIPARVIDAATRLKGIVKYGVGIDAIDIEAAMRKGVPVVNVPEYAEETV
                     AEGAIALMLALAKKFKPIEREMALRGWAWPTAPWLATDLAGKTLGLVGAGRIGT"
     gene            complement(464..1834)
                     /locus_tag="VFO53_06195"
     CDS             complement(464..1834)
                     /locus_tag="VFO53_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET8875904.1"
                     /translation="MNAKPAQPAPGDLSAEGDINLSPRRKRWEAERLAPATRALLAED
                     AKYFLHQSLSTPCFNALASASGIWLEDVEGRRYMDFHGNSVHQVGHGHPRVVQAVKDA
                     LDTLPFSPRRFTNEAAVTLARRLVDLAPGNLAKVLFAPGGTSAIGMALKLARVATGRH
                     KTVSMWDAFHGASLDAISVGGEALFRRGMGPLLSGTEHAQPCDPRACHFGCGGTCNAR
                     CAEYVDYVLGKEEDVAAVVVETIRSTDVQIPPRAYYETLRRACDRHGALLILDEIPIG
                     LGRTGAMFAFEHYGIVPDMVVLGKGLGGGVFPMAALIARDGLDVAAERALGHYTHEKS
                     SLGCAAALATLNVIDDERLLERANRLGAHALSRLGEMQRRLPIVASVRGIGLLLGVEL
                     VDPSSGAPARDAAERVLYDCLAQGLSFKVGQGNVLVLSPPLVIADQDLDRALDIVEAA
                     IGAASR"
     gene            2018..3178
                     /locus_tag="VFO53_06200"
     CDS             2018..3178
                     /locus_tag="VFO53_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET8875905.1"
                     /translation="MLAIAVLLVAFAGCDKREGATSARAAPAAAAPLAVTVKRAQLQR
                     VPLVLEAVGQAEGSREVEIRPRVAGILEKRLYTEGAKVDAGQALFRIDRAPFVLAVAQ
                     SRAALSQERARQELAQREADRLKALADTKAISRREYDTAVSAVKESAAAIEGAQAKLA
                     EAELNLSYTNLKAPIGGVTSRSVRSEGSLVAANTDLLTTITQVDPLWVRFSIAEGDFE
                     RIRDSAESTQVNIVDSKGAVAAGNGRLNFTASSVDPKIGTVQLRAEFANRDLRWLPGQ
                     FVKVRILAGAQDAFLVPQAALAQTERERTVWIAGADGKAVQRPVQTTTWLGSDWIVTG
                     GLAPGDLVIVDNLMKLRPGTAVEPRAAGAASQQAAASPVPPAEAPARPAKSR"
     gene            3191..>4123
                     /locus_tag="VFO53_06205"
     CDS             3191..>4123
                     /locus_tag="VFO53_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8875906.1"
                     /translation="MGNFSRFFIERPIFASVIAIIIALAGIIASQLLPVAQYPDIAPP
                     IVTITASYPGASAETLAKTVAAPIEEQLSGVENMIYFNSSAAADGSVGITATFEVDTN
                     IDNAVFQLNNRVQIALPRLPEEVRRNGVIVQKRSNDILLVVGLVSRSGAQKTTFMADY
                     ATVNMIDELKRVPGVGDVTLFGSGYAMRIWLQPDRMARLGVTPTDVAAAIRSQNQQYA
                     AGKIGAEPAPAGQSLVYTVTARGRLVTPEQFGDIVVRAGGPAGVLRIRDIARVELGAQ
                     SYDISTTVDGQPAVGMAVFLQTGANALDVGAAVKA"
BASE COUNT          667 a         1419 c         1409 g          628 t
ORIGIN      
        1 gtgccgatgc ggccggcccc gacgaggccg agcgtcttgc ccgcgagatc ggtcgcgagc
       61 cacggcgccg tcggccacgc ccatccgcgc aacgccattt cgcgctcgat cggcttgaac
      121 ttcttcgcaa gcgcaagcat cagcgcgatg gcgccttcgg caacggtctc ctcggcgtat
      181 tcgggcacgt tgacgacggg aacgcccttg cgcatcgccg cttcgatgtc gatcgcgtcg
      241 atgcccacgc cgtacttgac gatgcccttg agccgcgtcg ccgcgtcgat gacgcgcgcc
      301 gggatcgatg cgtagcacat gaggagcagg tcggcgtcgc gcacggcgcc ggcgagttcg
      361 acggtgctcg tcgtctcggg gagcacaacg agcgttgcgc cgtactcgcg cagccgtcga
      421 tcgacgcgcg gcagctcgag ctcggcgtcg gtgcgcacga cgttcatcgg ctcgcggcgc
      481 cgatggccgc ctcgacgatg tcgagcgcgc gatcgagatc ctggtcggcg atgacgagcg
      541 gcggcgaaag cacgagcacg ttcccctgcc ccaccttgaa cgacagcccc tgtgcgaggc
      601 agtcgtagag cacgcgctcc gcggcgtcac gcgccggcgc gccgctcgac gggtccacga
      661 gctcgacgcc gagcagcaga ccgatgccgc gcacgctggc gacgatcggc agccgccgtt
      721 gcatctcgcc aagccgggac agcgcatgcg cgccgaggcg gttcgcacgc tcgagcagcc
      781 gctcgtcgtc gatcacgttg agcgttgcga gcgccgccgc gcacccgagc gagcttttct
      841 cgtgcgtgta gtgaccgagc gcacgctcgg cggcgacatc gagcccgtca cgcgcgatca
      901 gcgcggccat cggaaacacc ccgccgccga gccccttgcc gagcaccacc atgtcgggaa
      961 cgatgccgta gtgctcgaat gcgaacatcg cgccggtgcg gccgagaccg atcggaatct
     1021 cgtcgaggat cagcagcgcg ccgtggcggt cgcatgcgcg gcgcagcgtc tcgtaatagg
     1081 cccgcggcgg aatctgcacg tcggtcgagc ggatcgtctc gacgacgacg gcggcgacgt
     1141 cctcttcctt gccgagcaca tagtcgacgt actcggcaca gcgcgcgttg caggtcccgc
     1201 cgcagccgaa atgacacgcg cgcgggtcgc agggttgcgc atgctcggtc ccggagagga
     1261 gcggtcccat gccgcggcgg aacagcgcct cgccgccgac ggaaatcgcg tcgagcgacg
     1321 ctccgtggaa tgcatcccac atcgagacgg tcttgtgccg gcccgtggcg acgcgcgcaa
     1381 gcttcagcgc catgccgatt gcgctggtgc ccccgggcgc aaagagcacc ttcgccaggt
     1441 tgccaggtgc aagatcgacg agccggcgcg cgagcgtcac cgccgcctcg ttggtgaagc
     1501 ggcgcggcga gaacggcagc gtgtcgagcg catccttgac ggcctgcacg acgcgcggat
     1561 ggccgtgtcc gacctggtgg acgctgttgc cgtggaagtc catgtagcgc cggccctcga
     1621 cgtcctcgag ccagatgccc gatgccgacg caagcgcgtt gaagcaaggc gtcgacagcg
     1681 actggtgcag gaagtacttc gcgtcttcgg cgagcagcgc acgcgtggcc ggcgccagcc
     1741 gttccgcctc ccagcgcttg cgccgcggcg aaaggttgat gtcgccttcg gcggacaaat
     1801 cgcccggtgc aggctgcgcc ggtttggcgt tcatgctgac agggcctttc gtgcaacgat
     1861 cgagcggcgc attctgtcac gcttcgcgcc agcgtccgta tgtgtcatat atcgtatgca
     1921 acgtatatat aatgccgcct gccgatcgtt cccgtcccgc tcgatgaatt cacgcctcgc
     1981 tgcaaatgcc ggtcgctgcg ccagacgcac ggcacccgtg ctcgccatcg ccgtcttgct
     2041 cgtcgcgttc gccggctgcg acaagcgcga aggtgccacc agcgcacgag cggcgccggc
     2101 ggcggcggcc ccgcttgccg tcaccgtcaa gcgtgcgcag ttgcagcgcg tcccgctcgt
     2161 gctcgaagcc gtcggccagg ccgaaggctc gcgcgaggtc gagatccgtc cgcgcgtcgc
     2221 gggcattctc gagaagcgcc tgtacaccga gggtgcgaag gtcgacgcag gacaggcgct
     2281 gttccggatc gatcgtgcgc cgttcgtgct tgcggttgcg cagtcccggg cggcgttgtc
     2341 gcaggagcgc gcgcggcagg agctcgcgca gcgcgaggcc gatcggctga aggcgctggc
     2401 cgataccaag gcaatcagcc gccgcgaata cgacaccgcg gtgtcggccg tcaaggagtc
     2461 ggccgcggcg atcgagggcg cgcaggcgaa gcttgccgag gccgagctca atctttcgta
     2521 cacgaacctg aaggcgccga tcggcggcgt gacgagccgc tccgttcgct ccgaaggcag
     2581 cctcgtcgcc gcgaacaccg acctgctgac gacgatcacg caggtcgatc cgctgtgggt
     2641 acgcttttcg atcgccgaag gcgatttcga gcgcattcgc gacagcgccg aatcgacgca
     2701 ggtgaacatc gtcgacagca aaggggcggt tgcggccggt aacggccggc tcaacttcac
     2761 ggcgtcgagc gtcgatccga aaatcggcac cgtgcagctg cgtgccgaat tcgcgaaccg
     2821 cgacctgcgc tggctgccgg ggcaattcgt caaggtgcgc attctcgcgg gcgcgcagga
     2881 tgcgttcctc gtcccgcaag cggcgctggc gcagaccgaa cgcgagcgca ccgtttggat
     2941 cgcaggtgcc gacggcaagg cggtccagcg tccggtgcaa acgacgacct ggctcggcag
     3001 cgactggatc gtgacgggcg gcctcgcccc cggcgatctc gtgatcgtcg acaacctgat
     3061 gaagctgcgc ccggggaccg ccgtcgaacc acgcgcggca ggtgccgcgt cgcagcaggc
     3121 ggccgcatcc ccggtgccgc ccgcggaagc gcccgcccgg cccgcgaagt cgcgctgacg
     3181 ggcaacgtcg atgggcaatt tctcgcgctt cttcatcgag cggccgatct tcgcgagcgt
     3241 catcgcgatc atcatcgcgc tcgccggcat catcgcatcg cagctcctgc cggtggcaca
     3301 gtacccggac atcgcgccgc cgatcgtcac gatcaccgcg agctatccgg gtgccagcgc
     3361 cgaaacgctc gcaaaaaccg tcgcggcgcc gatcgaggag cagctgtcgg gcgtcgagaa
     3421 catgatttat ttcaactcga gcgctgccgc cgacggctcg gtcggcatca cggcaacgtt
     3481 cgaggtcgac acgaacatcg acaacgcggt gttccagttg aacaaccgcg tgcagatcgc
     3541 gctgccgcga ttgcccgagg aagtgcgccg caacggcgtc atcgtgcaga agcgatccaa
     3601 cgacatcctg ctcgtcgtcg ggctcgtgtc gcgttcgggt gcgcagaaga cgacattcat
     3661 ggccgactac gcgacggtca acatgatcga cgagctgaag cgcgtgcccg gtgtcggcga
     3721 tgtgacgctg ttcggatcgg ggtacgcaat gcgcatctgg ctgcagcccg accgcatggc
     3781 gcgcctcggt gtgacaccga ccgacgttgc cgcggcgatc cgctcgcaga atcagcaata
     3841 cgcggcgggc aagatcggcg ccgagcctgc gccggcggga cagtcgctcg tctacacggt
     3901 gaccgcacga gggcgcctcg tgacgccgga gcagttcggc gacatcgtcg ttcgcgccgg
     3961 cggtcccgcc ggcgtcctgc gcattcgcga catcgcgcgg gtcgagctcg gcgcgcaaag
     4021 ctacgacatc agcaccaccg tcgatggcca gcccgcggtc gggatggcgg tgttcctgca
     4081 gacgggcgcc aatgcgctcg acgtcggcgc cgccgtgaag gcg
//
LOCUS       DASSVX010000142         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_238758, whole genome shotgun sequence.
ACCESSION   DASSVX010000142 DASSVX010000000
VERSION     DASSVX010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238758"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            522..620
                     /gene="ffs"
                     /locus_tag="VFO53_06210"
     ncRNA           522..620
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFO53_06210"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            967..2706
                     /gene="dnaX"
                     /locus_tag="VFO53_06215"
     CDS             967..2706
                     /gene="dnaX"
                     /locus_tag="VFO53_06215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET8875907.1"
                     /translation="MSYQVLARKWRPRAFVELVGQSHVVTALTNALSQGRLHHAYLLT
                     GTRGVGKTTLARILAKSLNCERGVSATPCGECAACRDIDAGRFVDLLELDAASNTGVD
                     NMREILDNARYAPTVGRYKVYLIDEVHMLSRGAFNSMLKTLEEPPEHVKFVLATTDPQ
                     KIPPTVLSRCLQFSLTPLPRTAIGERLAHILGEENVPFDPLALPQLAAAAQGSLRDAL
                     SLLDQAIAFGGGEVREQAVRAMLGVVDRGYLYRIIDALIASDGAALLAEADALTSSGQ
                     AFGAALDELATIFYRIAVVLAVPNAPPSDEPERIAAYAARFTAEEAQLGYQICAQGRA
                     DLALAPDEATGFSMTLLRLLAFEPASAPTARSRSGADVPSPAAKTAAQSTAVPATDAA
                     VRQSESNIAFTTPSRSVAASNASAPAERRVESRAAASAPATATPALQLPDTTAAWPAF
                     VASLRLTGMAAQLAAQSELKSVRGNALSLAIPASHKHLADKAYSDKLKAALDGATGRK
                     VLLAFEIGESSDGSLAALERRERAEAQAKGEAAFRNEPFVRDVVERFNATVRADSVTP
                     APRQTPLSKQEPT"
     gene            2703..3029
                     /locus_tag="VFO53_06220"
     CDS             2703..3029
                     /locus_tag="VFO53_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET8875908.1"
                     /translation="MMKNQLAGLMKQAQQMQENMQRAQEELARTEVEGQSGAGLVKIV
                     MTCRHDVKRVAIDPSLLGEDKDMLEDLVAAAFNDAVRRAEATTQDKMGNLTAGMPLPP
                     GFKLPI"
     gene            3029..>3327
                     /locus_tag="VFO53_06225"
     CDS             3029..>3327
                     /locus_tag="VFO53_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875909.1"
                     /translation="MAAAGAHDTARRSVRHEALSSLDELAGALRCLPGVGPKAAQRMA
                     LHLLQHDRDGAERLARALIDATRAIRHCERCNTFTEGELCTLCQSAKRDATQLCVV"
BASE COUNT          584 a         1140 c         1074 g          529 t
ORIGIN      
        1 ggacgcgtgc tctcgcagcc gctgaacgcc agcagcgaga gcgcggcgag gccaatcagt
       61 gaccggcgac accaactgct caaacgtcgt acaaacactc gatcgcgcat ggtcaatcgc
      121 cccgttgccg ggtgcgtcgt cgaaggtttg cggcgatctt gatgccgccg tacacgggcg
      181 cagccttgcc acgggctgcg aagcactcca gggtggtcgc ggacgcggca ccgcgtcctt
      241 cagcgacggt gtgattataa ccttgcgggt tgaagggcca atccgccgtc ccgccgagcg
      301 gcaaaaacgg cggcagggcg gcgatccccg gccaacgggc cgaatacgaa cacttgattg
      361 acggctgtta cgtgcgacag cttcgtggtc gcaggccgca ggctcgacgc gcggggcaac
      421 ccccggggcc ggcgacatcg tcccatcggg cttcggatca cccgctggcc gctgcgttga
      481 ccggttatac tggcggttcg cggaagttgc gcccgatcaa tggcgggtcg ccccgcattg
      541 caacgtcgtg aacctggtca ggtccggaag gaagcagcca cagcgattca ctgcaagtgc
      601 cgggggaacg gctcgcctcc cttgctcttt gtcgtccaga tcgacctgat actttgttgc
      661 tctccggctc caacgctcac gtacgtccat gtacgctgcg cgttgtcgcc ggctcgcgcc
      721 gcgtctcagg ccgatctggt gacaaatagc ccgcgctctg accgcgcgct cgtctcagcg
      781 cgatctgacg gcaaatggac ctcgactccg accgaacgcc caacgacgcg atctggtgac
      841 aaatagccgg cgcttcgacc gagcgcttga cggccgcggg atctggcggc acatagaccc
      901 cttactcgaa agccccgccg cgcatcggtg gggcgagcgc catccaggcg tagcgcccat
      961 tcgttcatga gctatcaagt cctcgcccgc aaatggcgac cgcgcgcatt cgtggagctg
     1021 gtcggccaga gccacgtcgt cacggcgctg acgaatgccc tcagccaggg caggctgcac
     1081 cacgcttacc tgctgaccgg tacacgcggc gtcggcaaga cgacgcttgc gcggatcctc
     1141 gccaagagcc tcaactgcga gcgaggtgtc tccgctacgc cgtgcggcga atgcgcagcg
     1201 tgccgcgaca tcgacgccgg ccgcttcgtc gacctcctgg aactggacgc ggcgtcgaac
     1261 accggcgtcg acaacatgcg cgagatcctc gacaacgcgc gctacgcgcc gacggtcggc
     1321 cgctacaagg tgtatctgat cgacgaagtg cacatgctgt cccgcggtgc tttcaactct
     1381 atgctgaaaa cgctcgagga gccgcctgag cacgtcaaat tcgtgcttgc gacgacagat
     1441 ccgcagaaga ttccgccgac ggtgctgtcc cgctgcctgc aattcagcct gaccccgctg
     1501 ccgcgcacgg cgatcggtga gcgcctcgcg cacatcctgg gtgaggaaaa cgttccgttc
     1561 gatccactgg cgttgccgca gctcgcggca gcggcgcaag gatcgctccg cgatgcgctg
     1621 tcgctcctcg accaggcgat tgcgtttggc ggtggcgagg tgcgcgagca ggccgttcgc
     1681 gcgatgcttg gcgtcgttga tcgcggctat ctatatcgga tcatcgacgc actcatcgca
     1741 tccgatggtg cggcgctcct cgccgaagcc gacgcgctca cgagttccgg gcaggcgttt
     1801 ggcgcagccc tcgatgagct cgcgacgatc ttctaccgga tcgccgtcgt tctcgcggtc
     1861 ccaaacgcgc cgccgtccga cgaacccgag cgcattgctg cctatgcggc acgcttcacc
     1921 gccgaagagg cgcagctcgg gtatcaaata tgcgcgcagg gccgcgccga tttggcactc
     1981 gcgcccgacg aggcgacggg tttctcgatg acgctgctgc gattgctcgc gttcgagccg
     2041 gcgtccgcgc ccaccgctag atcgcgcagc ggagcggacg taccgtcgcc ggccgccaag
     2101 accgcggcgc aatcgaccgc ggtgcccgca accgacgccg ccgtccgaca aagcgaatcg
     2161 aacatcgcgt tcaccacgcc gtcgcgcagt gtcgcggcgt ccaacgcaag tgcgccggct
     2221 gagcgtcgtg tcgagtcgcg cgccgctgca tcggcgccgg cgaccgcaac tcctgcgctg
     2281 cagttgcccg atacgacggc cgcctggccg gcgttcgtcg cgagcctgcg cctgaccggc
     2341 atggcggcgc agctcgccgc gcaatcggag ctgaaatcgg tcaggggcaa tgcgctgtcg
     2401 ctggcgattc ccgcttcgca caagcatctc gccgacaaag cgtattcgga caagctcaaa
     2461 gccgcgctcg atggcgcaac cggccgcaag gtgctgctcg cgttcgaaat cggcgaaagc
     2521 agcgacggct cgctcgctgc gctggaacgg cgcgagcgcg ccgaggccca agcgaaaggc
     2581 gaggcggcgt ttcgcaacga gcctttcgtg cgtgacgtcg tcgagcgctt caatgccacc
     2641 gttcgcgccg actccgtaac gccggcccca cgtcaaaccc cattgtccaa gcaggaaccg
     2701 acatgatgaa aaaccagctc gccggcctga tgaaacaggc gcaacagatg caggagaaca
     2761 tgcagcgcgc gcaggaggag ctcgcgcgca ccgaagtcga aggtcagtcg ggcgccggcc
     2821 tcgtcaagat cgtcatgacg tgccgccacg acgttaagcg cgtcgcgatc gatccgtcgc
     2881 tgctcggcga ggacaaggac atgctcgagg atctcgtcgc cgctgcattc aacgacgccg
     2941 tgcgtcgcgc cgaagcgacg acacaggaca agatggggaa cctgaccgct ggcatgccgt
     3001 tgccgccggg gttcaagctg ccgatctgat ggcggcagcg ggcgcgcacg ataccgcgcg
     3061 acgcagcgtt cgtcacgagg cgctgtcgag cctcgacgag ctggccggtg cgctgcgctg
     3121 ccttcccggc gtgggaccaa aagcggcgca gcggatggcg cttcacctgc ttcagcacga
     3181 ccgcgacggt gccgagcggc tcgcgcgcgc gctgatcgac gcgacgcgcg caatccgtca
     3241 ctgcgagcgc tgcaacacgt tcaccgaagg cgagctctgc acgctgtgtc aatcggcaaa
     3301 gcgcgacgcg acgcagcttt gcgtcgt
//
LOCUS       DASSVX010000143         5731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1238827, whole genome shotgun sequence.
ACCESSION   DASSVX010000143 DASSVX010000000
VERSION     DASSVX010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238827"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFO53_06230"
     CDS             <1..199
                     /locus_tag="VFO53_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8875910.1"
                     /translation="VVGAFVLSTISEVLATKVTSFASMFFGIVIVIAVVFMPRGIAHL
                     VENFRRTGWRYFAENVRTHRL"
     gene            206..967
                     /locus_tag="VFO53_06235"
     CDS             206..967
                     /locus_tag="VFO53_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875911.1"
                     /translation="MATPATNGGSENILDVRGVTKRFHGLVAVNDVTFHLRRGEILGL
                     IGPNGAGKTTLVGMVNGTLQPTTGDILFEGQSVRALPAFRRAHLGIGRTFQVMKPFPG
                     MSVLENASVGALFGAAGGEHNLRRAREQARESLEFVGLGKRIAQRADALGGPDRKRLE
                     LAKALAMQPRLLLLDEVMAGLNHVEIEEVIEDIRKVRDRGISILVIEHVMKAITSLSD
                     RVFVLHHGEKIVEGPTDEVLRDPRVVEAYLGKKRG"
     gene            980..1705
                     /locus_tag="VFO53_06240"
     CDS             980..1705
                     /locus_tag="VFO53_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875912.1"
                     /translation="MTPALLEIARLDAGYGEMQVLRGVSLVVRPAEIVAMVGGNGAGK
                     TTLLRAISRLIPSRGDIAFDGHPLAGTTADDVFAMGLVQVPEGRQLFDRMSVEENLRM
                     GAYRRRDASAIAGDLEKMYAIFPRLADRRRQLAGSMSGGEQQMCAMARALMARPRLMM
                     VDEMSLGLAPVIVDLLLETMTRIRAEGVTVLLVEQDVHAALSVADRGYVMETGEIVRE
                     GTARELASDPEVQRAYLGDVTGA"
     gene            complement(1789..3066)
                     /locus_tag="VFO53_06245"
     CDS             complement(1789..3066)
                     /locus_tag="VFO53_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET8875913.1"
                     /translation="MTIAFLFIILFVFMAIGMPIAVSLGLSSLLTILFFGHESMASQA
                     LKLYETASQFTLLAIPFFILSGAFMTTGGVARRMIRFANACIGHLRAGLGLASVLACM
                     LFAAVSGSSPATVVAVGSIVIGGMVRAGYSKEFAAGVICNAGTLGILIPPSIVMVVYA
                     AATSTSVGKLFIAGIVPGIVLGLMLMAAIYVRARIIDLPREQRATMKEVLTAGRESVW
                     GLLLLVIILGGIYGGIFTPTEAAAVAAVYAFVIAVFVYRDIGMRHVPKVLVDAGRVTV
                     MLMFIIANALLFAHVLTTERIPQVITEQIVAMGMTQWQFLIVVNVLLLIAGNFMEPTG
                     IILILAPILFPIATELGIDPVHLGVIMTVNMEIGMVTPPVGLNLFVTSGITGMSVVQV
                     VKAALPWLSVLFVFLIIVTYVPAITMWLPNLLY"
     gene            complement(3063..3608)
                     /locus_tag="VFO53_06250"
     CDS             complement(3063..3608)
                     /locus_tag="VFO53_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HET8875914.1"
                     /translation="MLRWYDRLEEGAIAFLLAVMTLITFGQVIARYVFDYSFVWALEL
                     VTFLFAWLIFLGMSYGVRVGSHIGVDALVKSLGARAARVVGAVATLCCIAYALICLWG
                     SWRYVATMHMIGTLAQDLPIPVWIPQIVLPLGFALLALRFAEVLYRILRGSDAHLLGD
                     EAAAILDQIDVPDANIDRRAP"
     gene            complement(3638..4624)
                     /locus_tag="VFO53_06255"
     CDS             complement(3638..4624)
                     /locus_tag="VFO53_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HET8875915.1"
                     /translation="MKLIRFAAIVAALAMVSAAQAQQPIVIKFSHVVAEKTPKGQAAL
                     KFKELAEKYLPGKVQVQVFPNSQLFGDDRELDALLLGDVQLLATSLSKFDRYTKKLQV
                     YDLPFMFENLAAVDRFQHSAAGKGLLDSMTGKGIKGLAYWHNGMKQMSSDKPQLKRPE
                     DVKGLKFRIQQSDVLEAQFRALGANPQKMAFSEVYQALQTGVVDGQENTWSNIYTQKF
                     YEVQKTIAETNHGVIDYMVITNAKWWDGLPADVKTGLQKAMDEATVYGNKLADDINQR
                     DKKLIIDAGKAKVQELSKDDLAAWQKAMAPVWKKFEGDIGKDLIDAAQKSNT"
     gene            4779..5696
                     /locus_tag="VFO53_06260"
     CDS             4779..5696
                     /locus_tag="VFO53_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HET8875916.1"
                     /translation="MDILEKARERIRGRGYRVVFPEGADERIVGAAKRLAADHLAEPI
                     VLDGSHAANVDRYARLYRDGRPDASEGVARRLAAKPLFHAGLMVKAGDADAMIAGVAN
                     PTGRVIEAGMMTIGLAPGIATPSSFFLIALPGRSLIYADCAVNADPSADALADIAIAS
                     AESGRRLLNDEPRVAMLSFSTKGSARHVRVDKVLRALAIVRERAPALAIDGELQADAA
                     LVPAVAAKKLKEAGPVAGRANVLIFPDLDSGNIAYKLTQYLAGAQAIGPILQGFARPI
                     SDLSRGASVDDIVATATLVLALTRSGSEL"
BASE COUNT         1008 a         1864 c         1933 g          926 t
ORIGIN      
        1 tgtcgtcggc gccttcgtgc tgtcgacgat ctccgaggtt ctcgccacca aggtcacgag
       61 cttcgcgagc atgttcttcg gcatcgtgat cgtgatcgcc gtcgtgttca tgccgcgcgg
      121 catcgcgcat ctcgtcgaga acttccggcg caccgggtgg cgctacttcg ccgagaacgt
      181 caggacacac cgattgtaga tcgcgatggc gactccggcg acgaacggcg gctccgaaaa
      241 cattctcgac gtgcgcggcg tcacgaagcg ctttcatggg ctcgtggccg tgaacgacgt
      301 gacgttccat ttgcgccgtg gcgagattct cgggctgatc ggcccgaacg gcgcgggcaa
      361 gacgacgctc gtgggcatgg tcaacgggac gctgcagccg acgaccggcg acatcctgtt
      421 cgaggggcag tcggtgcgcg cgctcccggc attccgccgt gcgcatctcg gcatcgggcg
      481 cacgttccag gtgatgaagc cgttccccgg catgagcgtg ctcgagaatg cgtcggtggg
      541 ggcgctgttc ggtgccgcgg gtggcgagca caacctgcgc cgcgcccgcg aacaggcgcg
      601 cgaatcgctc gagttcgtcg ggctcggcaa gcgcatcgcg cagcgcgccg atgcgctggg
      661 cggccccgac cgcaagcggc tcgagctcgc gaaggcgctc gcgatgcagc cgcgcctgct
      721 gctgctcgac gaggtgatgg cggggctcaa ccacgtcgag atcgaggagg tgatcgagga
      781 catccgcaag gtgcgcgatc gcggcatcag cattctcgtg atcgagcacg tgatgaaggc
      841 gatcacgagc ctgtccgacc gcgtgttcgt gctgcaccac ggcgagaaga tcgtcgaggg
      901 ccccaccgac gaggtgctgc gcgacccgcg cgtcgtcgag gcgtatctgg gaaaaaagcg
      961 tggctagagg acgcgtgcca tgaccccggc attgctcgag attgcgcgcc tcgacgcggg
     1021 ctacggcgag atgcaggtgc tgcgcggcgt cagcctcgtc gtgcgcccgg cggaaatcgt
     1081 cgcgatggtc ggcggcaacg gcgcgggcaa gacgacgctg ctgcgcgcca tttcacggct
     1141 gatcccgtcg cgcggcgaca tcgcgttcga cggacatcct ctcgcgggga ccacggcgga
     1201 cgacgtcttc gccatgggcc tcgtgcaggt gcccgagggc cggcagctct tcgaccggat
     1261 gagcgtcgag gaaaacctgc gcatgggcgc ataccggcgg cgcgatgcgt ccgcaatcgc
     1321 gggcgacctc gagaagatgt acgccatctt tccacggctc gccgatcgcc gccgccagct
     1381 cgccggcagc atgtcgggtg gcgagcagca gatgtgcgcg atggcgcgcg cgctgatggc
     1441 gaggccgcgg ctgatgatgg tcgacgaaat gagcctgggg cttgcgccgg tgatcgtcga
     1501 tctgctgctc gagacgatga cgcgcatccg cgccgaaggc gtcacggtgc tgctcgtcga
     1561 gcaggacgtg cacgccgcgc tgtcggtggc cgatcgcggc tacgtgatgg agacaggtga
     1621 aatcgtgcgg gaaggcaccg cgcgggagct cgcatccgat cccgaggtgc agcgcgccta
     1681 tctcggcgac gtcacgggtg cctgacttcg gggtcagagc tgtaagtcgc gctcgtgcag
     1741 cgacctttgc ccgactgcgt gccgcgactt gcagctctga ccccgaagtc agtacagcag
     1801 gttcggcagc cacatcgtga tcgccggcac gtaggtgacg atgatcagga acacgaacag
     1861 caccgacagc cacggcagcg cggccttcac gacctgcacg accgacatgc cggtgatgcc
     1921 cgacgtcacg aacaggttga ggcccaccgg cggtgtcacc atcccgatct ccatgttgac
     1981 cgtcatgatg acgccgagat ggacgggatc gatgccgagc tcggtcgcga tcggaaacag
     2041 gatcggcgcg aggatcagga tgatgccggt cggctccatg aagttgccgg cgatcagcag
     2101 cagcacgttg acgacgatca ggaactgcca ctgcgtcatc cccatcgcga cgatctgctc
     2161 ggtgatcacc tgcggaatgc gctcggtcgt cagcacgtga gcgaacagca gcgcgttggc
     2221 gatgatgaac atcagcatga cggtgacacg tcccgcgtcg acgaggacct tcggcacgtg
     2281 acgcatcccg atgtcccggt acacgaatac ggcgatgacg aaggcgtaga cggccgcgac
     2341 ggcggcagcc tcggtcggcg tgaagatgcc gccgtagatg ccgccgagga tgatcacgag
     2401 cagcagcaac ccccacaccg actcgcggcc ggccgtcagc acttccttca tcgtggcgcg
     2461 ctgctcgcgc ggcaggtcga tgatgcgcgc gcggacgtag atcgcggcca tcagcatcag
     2521 cccgagcacg atgccgggaa cgatgccggc gatgaatagc ttgcccaccg acgtcgacgt
     2581 ggccgccgca tagaccacca tgacgatcga cggcggaatc aggatgccaa gcgtgcccgc
     2641 gttgcagatg acgcccgcgg cgaactcctt cgagtagccg gcacgcacca tgcccccgat
     2701 gacgatcgag ccgacggcga cgaccgtcgc gggcgacgaa cccgacaccg cggcgaacag
     2761 catgcaggcc agcaccgagg cgaggccgag ccccgcacgc agatgcccga tgcacgcatt
     2821 cgcgaaacga atcattcgcc gggcgacgcc gcccgtcgtc atgaaggcgc cggagaggat
     2881 gaagaacgga atcgcgagca gcgtgaactg cgacgcggtc tcgtacagct tcagcgcctg
     2941 cgacgccatc gactcgtggc cgaagaacag gatcgtcagg agcgacgaca ggccgagcga
     3001 cacggcgatc ggcatgccga tcgccatgaa cacgaacagg atgatgaaca ggaacgcgat
     3061 cgtcacggcg cccgccggtc gatgttggca tccgggacgt cgatctggtc gaggatcgcc
     3121 gccgcctcgt caccgagcag atgggcgtcg ctcccgcgca gaatccggta gagcacctcg
     3181 gcgaagcgca gcgcgagcag cgcgaagccg agcggcagca cgatctgcgg aatccacacc
     3241 ggaatcggca ggtcctgcgc gagcgtgccg atcatgtgca tcgtcgcgac gtagcgccag
     3301 ctcccccaca ggcagatcag cgcgtaggcg atgcagcaga gcgtcgcgac cgcgccgacg
     3361 acgcgtgctg cacgtgcgcc gagcgacttg acgagcgcgt cgacgccgat gtgcgagccg
     3421 acgcgaacgc cgtacgacat tccgaggaag atcagccacg cgaacaggaa cgtgacgagc
     3481 tcgagcgccc acacgaagct gtagtcgaag acgtagcgcg cgatcacctg accgaacgtg
     3541 atcagcgtca tgacggcaag gaggaaggcg atcgcgcctt cctccagccg gtcgtaccag
     3601 cgcagcacgt gcgtgccggt ccgtggggcg ggccgggcta cgtgttcgac ttctgtgccg
     3661 cgtcgatcag gtccttgccg atgtcgccct cgaacttctt ccagaccggc gccatcgcct
     3721 tctgccacgc ggcgaggtcg tccttcgaca attcctgcac cttggccttg ccggcgtcga
     3781 tgatcagctt cttgtcgcgc tggttgatgt cgtcggcaag cttgttgcca tagacggtgg
     3841 cctcgtccat cgccttctgc aacccggtct tgacgtcggc gggcaggcca tcccaccact
     3901 tggcgttcgt gatcaccatg tagtcgatca cgccgtggtt ggtctcggcg atcgtcttct
     3961 gcacttcata gaacttctgc gtgtagatgt tcgaccacgt gttctcctgg ccatcgacga
     4021 caccggtctg cagcgcctgg tagacctccg agaacgccat cttctgcggg ttcgcgccga
     4081 gcgcgcggaa ctgcgcctcg agcacatccg attgctggat gcggaacttg agacccttca
     4141 cgtcctccgg gcgcttgagc tgcggcttgt cgctggacat ctgcttcatg ccgttgtgcc
     4201 agtaagcgag gcccttgatg cccttgccgg tcatcgaatc gagcaggccc ttgccggcgg
     4261 cgctgtgctg gaagcggtcg accgccgcga ggttctcgaa catgaacggc aggtcgtaca
     4321 cctgcagctt cttcgtgtaa cggtcgaatt tcgacagcga cgtcgcgagc agctgcacgt
     4381 cgccgagcag cagcgcgtcg agctcgcggt cgtcgccgaa aagctgcgaa ttcgggaaca
     4441 cctgcacctg caccttgccg ggcaggtatt tctcggcgag ctccttgaat ttcagcgccg
     4501 cctggccctt cggcgtcttc tcggcgacga catggctgaa cttgatgacg atcggctgct
     4561 gcgcctgcgc cgccgatacc atcgcaaggg ccgcaacgat ggcggcgaat cgaatcagtt
     4621 tcatgctggc tcctccgaaa gatggcaagc ccgcggcctt gttcgaatcg gcggccggga
     4681 tcctgtcggc gcatgttcgc gccgccgcaa gagtagcatg cgtagcctgc aaaaccgatc
     4741 atggccgcgg ctcgaaacgg catttgcaag gcgaagcgat ggacattctg gagaaggcgc
     4801 gggagcgcat ccgcggacgg ggttaccggg tggtgtttcc cgaaggcgcc gacgagcgca
     4861 tcgtcggcgc cgcaaagcgg ctcgccgccg accacctcgc cgagccgatc gttctcgatg
     4921 gctcgcacgc ggcgaacgtc gatcgctacg ccaggctcta tcgcgacgga cgcccggacg
     4981 cgagcgaagg ggtggcgcgg cgcctcgcgg ccaagccgct cttccatgcg ggactgatgg
     5041 tgaaggcggg cgacgccgac gccatgatcg ccggcgtggc caatccgacc gggcgcgtga
     5101 tcgaggccgg gatgatgacg atcggccttg cacccggcat cgcgacgccg tcgagcttct
     5161 tcctgatcgc gctgcctgga cgctcgctga tctacgccga ctgcgccgtc aacgccgacc
     5221 cttccgcgga tgcgctggcc gacatcgcaa tcgcatcggc ggaaagcggc cggcggctgt
     5281 tgaacgacga gccgcgcgtc gcgatgctgt cgttctcgac gaagggcagc gcgcgccacg
     5341 tgcgtgtcga caaggtgctg cgggcactcg ccatcgtgcg cgagcgtgcg ccggcgcttg
     5401 cgatcgacgg cgaattgcag gccgacgcgg cgctcgtgcc cgccgtcgcg gcgaagaagc
     5461 tcaaggaggc agggccggtc gccggtcgcg ccaacgtgct gatctttccc gatctcgaca
     5521 gtggcaacat cgcgtacaag ctgacgcagt acctggccgg cgcgcaggcc atcggtccga
     5581 tcctgcaggg cttcgcgcgg ccgatcagcg acctgtcgcg cggcgcgagc gtcgacgaca
     5641 tcgtcgcgac ggcgacgctc gtgctggcgt tgacgagatc ggggtcagag ctgtaattat
     5701 cagcgaacta tccgtcgata gcccccggtg t
//
LOCUS       DASSVX010000144         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_138903, whole genome shotgun sequence.
ACCESSION   DASSVX010000144 DASSVX010000000
VERSION     DASSVX010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138903"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="VFO53_06265"
     CDS             <1..112
                     /locus_tag="VFO53_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET8875917.1"
                     /translation="PANDAWIAALALQHRLPVLSRDEHFDVVPHLARTSW"
     gene            208..1269
                     /gene="speB"
                     /locus_tag="VFO53_06270"
     CDS             208..1269
                     /gene="speB"
                     /locus_tag="VFO53_06270"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HET8875918.1"
                     /translation="MNSDKLAALRRKYTDAKGGDIFDPEFAAVAAQVFTNPERRKWPF
                     ADVATLLAAPYRPDAPSLPDFGGLDVALVGVPMDLGVTNRAGARLGPRAVRDVERIGP
                     YDHALRIAPSVHLSVADVGDVPMQSRFSLDQCHADIEAYFTRVCAAGVVPLAVGGDHS
                     ITHSILKAVGRDRPVGMVHIDAHCDTAGEYEGAKFHHGGPFRHAVLAGVLDPERSIQI
                     GIRGGAEYLWEFSYESGMTVLHIEDVMTMGIPAVVARAREVVGSGPIYVTVDVDAIDP
                     GFAPGTGTPEVGGLTPREVLAILRGLAGLNVVGGDVVEVAPQYDATTNTAQVGAQCLF
                     DLLCLVAIDKRQHVRANGR"
     gene            1247..2182
                     /locus_tag="VFO53_06275"
     CDS             1247..2182
                     /locus_tag="VFO53_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HET8875919.1"
                     /translation="MSEPTVDSGAMPVEFVKSGTWLGVASGMPSTHCRAAVLGIPFDC
                     GTHPHRIGARLGPSAIREQSTNLRRYDPRTNVDIVERLGLVDCGDVPVTPGLIDPSFA
                     AIEAGVAAVIAKNVVAVTMGGDGAVTLPQVRALSHHTPGLCTVHIDAHTDAYPIDGYN
                     TATSFRHAAQEGLVDVARSFHIGMRGPTMVPGVYEFCKELGYNMITMQGLRESGFERV
                     AAGVIETIGDRPVYLCFDMDFFDPSVAPGVCTPTWGGCTAHEGLAFLEMLHRLDTRVL
                     DVNTVSPPHDCAGMTAFLAATVMWTFLSGLANERA"
     gene            complement(2222..3064)
                     /locus_tag="VFO53_06280"
     CDS             complement(2222..3064)
                     /locus_tag="VFO53_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET8875920.1"
                     /translation="MTFDLETDPRRVPLFEERTWPDIAAAVAADAPVIVPMGATEQHG
                     GHLPLGADTIQGIDMCRRTAAVLAREGVPLLVGPAIPFGPLPFLSESPKGFPGTIVVS
                     NDTLRMLTRDVCVSLIAHGFRRIYLLLANVESEYAMHIVAKELTETTPANIVTLNWLI
                     GARPGYKGILKSDRPQGHGGEGETARLLATAPHLVRMDEARPYHPNLPAQPDVHGDAL
                     PYLGGAVGRYRFDGNEFDGFVGGITGDPQLATAETGEKSYALIASWIASVVRFDLRPK
                     LQST"
     gene            complement(3073..>3458)
                     /locus_tag="VFO53_06285"
     CDS             complement(3073..>3458)
                     /locus_tag="VFO53_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013512744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HET8875921.1"
                     /translation="SCFKDDVHPYRHTLRDGSPGPIELPEHPSLDDWSYGTSHLRFPK
                     PIFGADHVLGIWQNEFAMIREWGGVFVLVMHPQVTGRPMRIAILRRFIEYTRQFDDVW
                     YATGREIADAYAAQEADASRRLARS"
BASE COUNT          549 a         1149 c         1155 g          605 t
ORIGIN      
        1 cccggccaac gatgcctgga tcgcggcgct tgcgttgcag caccggctcc ccgtacttag
       61 ccgcgacgag catttcgatg tcgtaccgca ccttgcgcgg accagctggt agccccggca
      121 ccggcggggc ggcgcggaac gattgcattc gaccataaaa gcgcgtcgta cgtcgcgaca
      181 gcccacattg acatataggt gcagcacttg aactccgaca aactcgccgc gctgcgccgc
      241 aagtacaccg acgccaaggg cggcgacatc ttcgatcctg aattcgccgc ggtggctgcg
      301 caggtcttca ccaaccccga gcgcaggaag tggccgttcg ccgacgtcgc gacgctgctc
      361 gccgcgccgt accgtcccga tgcgccgtcg cttcccgatt tcggcggcct cgacgtcgcg
      421 ctggtcggtg tgccgatgga cctcggcgtc accaatcgcg ccggtgcccg gcttgggccg
      481 cgtgcggtgc gcgatgtcga gcggatcggt ccgtacgatc atgcattgcg catcgcgccg
      541 agcgttcacc tttccgtcgc cgacgtcggc gacgtgccga tgcagagccg gttcagcctc
      601 gatcagtgcc acgccgacat cgaggcgtac ttcaccaggg tctgtgccgc gggcgtcgtg
      661 ccgctggccg tcggtggcga tcattcgatc acgcattcga tcctgaaggc ggtcggccgc
      721 gaccggccgg tcggcatggt gcacatcgac gcgcactgcg atacggcagg cgaatacgag
      781 ggcgccaaat ttcatcatgg cgggccattc cggcatgcgg tgctcgcggg cgttctcgat
      841 cccgaacgtt cgatccagat tggcatccgc ggcggcgccg aatatctgtg ggagttctcg
      901 tacgaaagcg gcatgacggt gctccacatc gaggacgtaa tgacgatggg cattcccgcc
      961 gtcgtggccc gcgcacgcga agtcgtcggt tccgggccga tctacgtgac ggtcgacgtc
     1021 gacgcgatcg atcccggatt cgcgccgggc accggcacgc cggaggtcgg cgggctcaca
     1081 ccgcgggagg tgctcgccat cctgcgcggc ctggcgggac tcaacgtcgt cggtggtgac
     1141 gtcgtcgagg tggcgccgca gtacgacgcg acgaccaata ctgcgcaggt cggcgcgcag
     1201 tgcctgttcg atttgctgtg cctcgtggcc atcgacaagc gccaacatgt ccgagccaac
     1261 ggtcgatagc ggcgcaatgc cggtcgaatt cgtcaagtcc gggacctggc tcggcgtggc
     1321 gtcgggcatg ccttccaccc attgccgcgc ggccgtgctc ggcatcccgt tcgactgcgg
     1381 cacgcacccg catcgtatcg gcgcgcgtct cggtccgtcg gcgatacgcg agcaatcgac
     1441 caacctgcgg cgctacgatc cgcgcaccaa cgtcgatatc gtcgagcgtc taggactcgt
     1501 cgattgcggc gacgtccccg tcacaccggg cctcatcgat ccctcgttcg ccgcaattga
     1561 agcgggtgtc gccgccgtca tcgcgaagaa cgtcgtcgcc gtgaccatgg gtggcgacgg
     1621 ggcggtcacg ctgccgcagg tgcgtgcgct gtcgcaccac acgccggggt tgtgcacggt
     1681 gcacatcgat gcgcataccg atgcgtatcc gatcgacggc tacaacaccg cgaccagctt
     1741 ccggcatgcg gcgcaggagg gactggtcga tgtcgcgcgc tcgtttcaca tcggcatgcg
     1801 cggcccgacg atggtccccg gcgtttacga gttctgcaag gagctcggct acaacatgat
     1861 cacgatgcag ggactgcggg aaagcggttt cgagcgtgtt gcggcaggcg tgatcgagac
     1921 catcggcgat cggcccgtct atctgtgctt cgacatggac ttcttcgatc cgtccgtcgc
     1981 gccgggggtg tgcacgccca cgtggggcgg ctgcaccgcg cacgagggat tggcgttcct
     2041 cgagatgctg catcggctcg acacgcgcgt gctcgacgtg aacacggtca gcccgccgca
     2101 cgattgcgcc ggcatgacgg catttctcgc ggcaacggtg atgtggacgt tcctgtccgg
     2161 tctcgcgaac gagcgcgcct gaagcgcacg cgcatctcga gcgcctgcgc accgcaaccg
     2221 ctcaagtcga ttgcagcttc ggccgcagat cgaagcgcac gacgctcgcg atccagctgg
     2281 cgatgagggc gtagctcttc tcgccggttt ccgcggtcgc gagttgcgga tcgccggtga
     2341 tcccgccgac gaagccgtcg aattcgttgc cgtcgaatcg ataacgtcct acggcgccgc
     2401 caagatacgg tagtgcatcg ccgtgcacgt cgggctgcgc cggcaggttc ggatggtatg
     2461 gccgcgcctc gtccatgcgc accagatgcg gagcggtcgc aagcagccgc gccgtctcgc
     2521 cttcaccgcc atgcccctgc gggcggtccg acttgaggat gcccttgtag ccgggccgtg
     2581 cgccgatcag ccagttgagc gtgacgatgt tcgcgggggt ggtctccgtc agctccttgg
     2641 cgacgatgtg catcgcatac tcgctttcga cattggcgag cagcaggtag atgcgccgga
     2701 agccgtgcgc gatcagcgac acgcacacgt cgcgcgtgag catgcgcagc gtgtcgttcg
     2761 acacaacgat cgtgccggga aagcccttcg gtgattcgct caggaagggc agggggccga
     2821 acgggatcgc gggaccgacc agcagcggca cgccttcgcg tgcgagaacc gcggcggtgc
     2881 ggcggcacat gtcgatgccc tgtatcgtgt cggcgccgag tggcaggtgc ccgccgtgct
     2941 gctcggtggc gcccatcggc acgatgaccg gcgcatcggc cgccacggcc gccgcgatat
     3001 cgggccacgt gcgttcttcg aacaacggca cgcggcgagg atcggtctcg agatcgaagg
     3061 tcatcggcga ttctacgatc gcgccagtct gcgactggcg tccgcttcct gtgccgcgta
     3121 cgcgtcggcg atctcgcgcc ctgtcgcata ccagacgtcg tcgaactggc gcgtgtactc
     3181 gatgaacctg cgcaggatgg cgatgcgcat ggggcgcccc gtcacctgcg ggtgcatcac
     3241 cagcacgaac acgccgcccc actcgcggat catcgcgaac tcgttctgcc agatgccgag
     3301 cacgtgatcg gcgccgaaga tcggcttcgg aaaccgcagg tgactcgtgc catagctcca
     3361 gtcgtcgagc gacggatgct cgggcagctc gatcggaccg ggtgagccgt cgcgcagcgt
     3421 gtgccgatat ggatgcacgt catccttgaa gcagctgt
//
LOCUS       DASSVX010000145        16466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_438694, whole genome shotgun sequence.
ACCESSION   DASSVX010000145 DASSVX010000000
VERSION     DASSVX010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 16466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16466
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438694"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..175
                     /locus_tag="VFO53_06290"
                     /pseudo
     CDS             35..175
                     /locus_tag="VFO53_06290"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            311..745
                     /locus_tag="VFO53_06295"
     CDS             311..745
                     /locus_tag="VFO53_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET8875922.1"
                     /translation="MLPPPTEGQDIVADYRRLGLTLRRHPLALLRRQLRARRLATADD
                     VCAAAHGRLVRTAGIVIGRQRPDTASGVVFVTLEDETGASNVIVRREIGERQRRELLG
                     ARLLGVYGTVEREGEVVHILAGRLVDLTPMLGALPTRSRDFH"
     gene            888..2324
                     /locus_tag="VFO53_06300"
     CDS             888..2324
                     /locus_tag="VFO53_06300"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8875923.1"
                     /translation="MKSEFDLAVIGGGAGGLVVAAGAAKLGAKVALIEKDRLGGDCLW
                     HGSVPSKTLRKSARVAYTMRHADRFALAPADPRPDLARVMEHVAEVVAGIAMQDSPEH
                     FTSLGIDVIHGGGRFVSPTAFEVGGRIITARHFVLATGSRPAIPPIAGLAYVTYLTNQ
                     TVFDLREPVPRLVIVGAGPNGSEMAQAFRRLGSDVIVVDMANKILPREDADVAAVVQR
                     QLEAEGVRYRLGITVGGVMPGDPHAGRIRMTLRTANGAVEQLAATHLMLAAGRMPNIE
                     GLGLDAAGVQLDNGRLVVDDVLATTNPRIRAVGDVAGTFPYTHVAEHQADIVLRQTLH
                     RMSWAKPSPVVPWCTYTDPELARVGLSETQARRHGIDYRIYRFPFAQVDRARAEGETE
                     GFAKLVTDRAGRLLGAAIVGADAGELIAECVLAIGRRMKVDDLSAAIHAYPTRSEVVF
                     RAADERRRQAAGGSSRVRMRRLFGLGAT"
     gene            complement(2338..3612)
                     /gene="fahA"
                     /locus_tag="VFO53_06305"
     CDS             complement(2338..3612)
                     /gene="fahA"
                     /locus_tag="VFO53_06305"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HET8875924.1"
                     /translation="MTPGDAKQKSFVTVAKDSPFPIQNLPYGVFTTPANYSPRVGVAI
                     GDQVLDLSVLEQAGLLDLVPGERLFNRPTLNPFIERGPDTWRRARIAISDLLRAENGR
                     LRDDARLRARAFASQAAAKMLLPITVGAYTDFYSSKEHATNVGSMFRDPAHALLPNWL
                     CMPIAYNGRASSIVVSGTPVLRPNGQSKRPDAQLPLFGPSQRLDFELEVAFVVGVGNA
                     LGEPIPVGSALSNVFGMVLMNDWSARDLQQWEYIPLGPFNSKTFATSISPWIVTMEAL
                     EPFRVDGPVQDPPPLPYLATSGPQAYDIHLEAALAAAGKAPATISRTNFRTMYWSIAQ
                     QLAHHTVSGCNTRVGDLMGSGTISGAAPDTCGSLLEITKNGARPLHLPDGSERTFLED
                     DDELILTGWAQAKDYRIGFGEVRGRIVAARSS"
     gene            complement(3609..4904)
                     /gene="hmgA"
                     /locus_tag="VFO53_06310"
     CDS             complement(3609..4904)
                     /gene="hmgA"
                     /locus_tag="VFO53_06310"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HET8875925.1"
                     /translation="MNPRYQSGFGSEFATEALAGALPIGRNSPQRPPYGLYAEQLSGT
                     AFTAPRHANRRTWLYRIRPAVLHGAFTPFAQPRFATAPLDDTHTSPNQLRWDPFPLPD
                     APADFVDGIATIAGNGNAAAQIGIAIHVFACNRSMRGRYVYDADGELLIVPQQGALRI
                     ATELGTLDVAPGEIALVPRGVRFAVELADERSPARGYVCENYGAQFRLPELGPIGANG
                     LANPRDFLAPVASYSESEGDFELVAKFAGRLWRAPIDHSPLDVVAWHGNYTPYKYDLA
                     RFNAINTVSFDHPDPSLFTVLTSPSDTAGTANVDFVTFPPRWLVAEDTFRPPWFHRNA
                     MSEYMGLIHGTYDAKSEGFLPGGGSLHNAMSGHGPDAETFERASHAELTPQRLGDTLA
                     FMFESRYVIHPTPYALSSPQLQRDYPACWRGLARHFDAP"
     gene            complement(4901..5938)
                     /locus_tag="VFO53_06315"
     CDS             complement(4901..5938)
                     /locus_tag="VFO53_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET8875926.1"
                     /translation="MDSVDLEVLRNSAAWVEDGRRVLLVTVVKTWGSSPRPEGAMLAV
                     REDGLVVGSVSGGCIEDDIVDRSRREGQRATRCEVVTYGVSADEARRFGLPCGGTIEL
                     VLEPLTRESGMRALLREVETGHLVARRLDLASGFATLHPAQPSDGLAFDGKVLRTIHG
                     PRYRMLVIGASQLSKYLAQIAVGLGYDVTICDPREEYSETWDVPGVRLVRTMPDDTVQ
                     AMRLDERSAVIALTHDPKLDDLALMEALKSPAFYVGAIGSRANNEKRRERLREFDLSD
                     DEIARLHGPVGLYIGSRTPPEIAISILAEVTAIKNGVDAGHIRSVAAAKAALAQTPAC
                     SVLDEAADSTR"
     gene            complement(5955..6575)
                     /locus_tag="VFO53_06320"
     CDS             complement(5955..6575)
                     /locus_tag="VFO53_06320"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HET8875927.1"
                     /translation="MELSNTRIVPAPPVAVWAALNDPAVLKACVPGCESLERTGEDAY
                     ELVMSARIGPVSARFTGRMTMTDIDAPRAYTLNFEGQGGAAGFARGDARVTLAPEGER
                     QTALTYVAKAQIGGKLAQVGSRLVNGVAAKMSDDFFARFVARFAPPDEPAPVGASATV
                     AAQETMAETAAPPSLAPPGGSQWIRYAAIVAIIVVLVVLYARGYRP"
     gene            complement(6589..7728)
                     /locus_tag="VFO53_06325"
     CDS             complement(6589..7728)
                     /locus_tag="VFO53_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET8875928.1"
                     /translation="MLRRAGLPVGPAKVIDALAAIDAVGVDNRGDFREALAAVLVSRR
                     EHLPIFEQAFDMFWRNPRLLEKMIAALLPKVQGRVSDAAGDLPARLAQAMLPPPATAT
                     DDAQETELDAALSFSAREVLQRKDFATMTVDELAEVKALLRRMRLPLPARRVRRTIAA
                     PRGHAIDLRATLRGSVGAGGALTPLRFRTRRRRPPPLCVLCDVSGSMDRYARMLLHFL
                     HAVTNDRHRVQVLTFGTRLTNITRHLRHRDVDVALARVSSAVEDWAGGTRIGTCLMEF
                     NRKWSRRLLGQNAVVLLISDGLDADAGEGLAFEAERLAKSCARLVWLNPLLRYAGFEA
                     RPAGIRALLPHVDDFLPVHNMETLTDLAHALAHPRHRGTGTACAA"
     gene            complement(7913..8812)
                     /locus_tag="VFO53_06330"
     CDS             complement(7913..8812)
                     /locus_tag="VFO53_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET8875929.1"
                     /translation="MQAPFNVDATLALLEQHDYVAERRLATAVFLALKLQRPLFLEGE
                     AGVGKTEIAKVLAAGLARPLVRLQCYEGLDTSAAVYEWNYPRQMIEIRLAEAAGGVER
                     GELSHDIFTTRFLLRRPLLQALSPEDGAPPVLLIDELDRADEPFEAYLLEVLSDYQVT
                     IPELGTIRAQTPPVVVITSNRTREIHDAIKRRCLYHWVDYPDAARELAIVARKCPAAS
                     ASLAREVVAFTQRLRTLDLFKAPGIAETLDWAEALVALDRITLDPQTVADTLGALLKY
                     QDDVAAITPDVAAKLVREAQAAA"
     gene            complement(8950..9210)
                     /locus_tag="VFO53_06335"
     CDS             complement(8950..9210)
                     /locus_tag="VFO53_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HET8875930.1"
                     /translation="MSRPSRIPQFESEAAERAYWESHDSSRVLDWSQARRVNLPNLKP
                     TTQSISLRLPNHMLERIKAAANARDVPYQSLIKIWLEEKLSV"
     gene            complement(9194..9493)
                     /locus_tag="VFO53_06340"
     CDS             complement(9194..9493)
                     /locus_tag="VFO53_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HET8875931.1"
                     /translation="MIDFDAIAGFQWDDGNARKNERHGVSQGEAEQAFFDTRLLIMEG
                     TQHSLREPRYHALGMTREGRHLHITFTLRDGATLIRVIAARDMHRKERAIHEQAV"
     gene            complement(9679..10473)
                     /locus_tag="VFO53_06345"
     CDS             complement(9679..10473)
                     /locus_tag="VFO53_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET8875932.1"
                     /translation="MYAIDYQQPKTLADAVSVLASTGGRPLAGGQSLVAAMKLRLSQP
                     GTLVDLAGIADLRGIRRDGERLVIGAMTRHAEIASSDLVRTAMPALCDLAEGIGDRQV
                     RNMGTLGGSLANNDPAADWPAAVLATGATIRTHQRAIAADDFFKGMFETALGPDEIIT
                     AVDFPRVERAAYVKFPNPASRFALVGVFVARSSNGDVRVAVTGAAPSVFRATGIESAL
                     ARSFTPVAAKGVAIDASGLNNDLHASAEYRAHLVSVMASRAVAAIA"
     gene            complement(10483..12867)
                     /locus_tag="VFO53_06350"
     CDS             complement(10483..12867)
                     /locus_tag="VFO53_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET8875933.1"
                     /translation="MGTNDTTTLASVIGQPVLRKEDARFLTGTGQYTGDVSMPRQTFA
                     CFLRSPHAHAVIRSIDVVKAKTLPGVVEIYTGADLTGVNGLPCGWLITGTDGKPMNEP
                     PHPVLAQGKVRYVGDAVALVIAESIAEAKDAAEQIVVDYDVLPAVVNPVAALAPGAPQ
                     IHEQAPGNKCYTWAIGDKAAVDAAFAGAAHVTKIDIVNNRLIPNAIEPRAAVASYDRA
                     DDAYTLYVASQNPHVERLLMTAFVLGLPEHKVRVIAPDVGGGFGSKIYLYPEETAMVF
                     ASKRINRPIKWAAERSESFLSDAHGRDHVTHAELALDKDGRFLALRVQTTANMGAYLS
                     TFASCIPTILYATLLAGQYTTPAIHCEVTAAFTNTAPVDAYRGAGRPEATYVVERIVH
                     QAAAEMGIAQDEIRRRNFIRDFPHQTPVALNYDTGNYDQTLDAAIDMADVAGFAARRA
                     EAQKRGKLRGLGYAAYIEACGLAPSNIAGSLGARAGLFEAGEVRVHPTGSVTVFTGSH
                     SHGQGHETTFAQVVAAHLGVPVDNVSVVHGDTGRVPFGMGTYGSRSLAVGGSAIVKAL
                     DKIVAKGKKIAAHLLEAAESDIEFRDGKFTVAGTDRSKTFGEIALTAYVPHNYPLATL
                     EPGLNETAFYDPTNFTYPAGTHICEVEIDPDTGVVDVVAFTACDDFGNIINPMIVEGQ
                     VHGGVAQGLGQALLEHCVYDPDSGQLLTGSMMDYALPRADDLPSFNVGTKVTPCTHNP
                     LGVKGCGEAGAIGAPAAMMNAVMDALAPLGVAHLDMPASPHRVWGAIRQARGHA"
     gene            complement(12877..13350)
                     /locus_tag="VFO53_06355"
     CDS             complement(12877..13350)
                     /locus_tag="VFO53_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET8875934.1"
                     /translation="MSVPLEMSVNGKPVRADVDARTLLVDFLRNNLALTGTHVGCDTA
                     QCGACTVHLDGQAVKSCNVLAVQAQGASVVTIEGLIAEDGTMHPMQSAFRDCHGLQCG
                     FCTPGMVMSAIDLVNRHPGADEATIRAGLEGNLCRCTGYHNIVKAVRQAAVVMGK"
     gene            13629..14015
                     /locus_tag="VFO53_06360"
     CDS             13629..14015
                     /locus_tag="VFO53_06360"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HET8875935.1"
                     /translation="MLHERFKRTIGIAGAASVAILAAGCVTVATPSPTGRLAVANAAI
                     ADAISADASQYDAADLDKARRKLARAHDEAAQGDYGVARDLAEEAEVDARLAATRARS
                     TKAAHAAAAVQESIRALDDEIARTPH"
     gene            14028..15044
                     /locus_tag="VFO53_06365"
     CDS             14028..15044
                     /locus_tag="VFO53_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET8875936.1"
                     /translation="MKAWKARMGIVVPAFALGACAVLPAHDAEVDDARAAVLAAQNDP
                     QVVSLAPVELNKAVDAMRQADAAWSNRGSIEEVHHLAYLAWTHAAIATETARLKTAEA
                     SVASANAERDRVRLAARTRDAERAQRQAARAQQDAARAQAQADANRANALAAQQQAAD
                     AARQAQAARDQAEASRAQAADAQARAQSLQSSLAELQARPTDRGMVVTMGDLLFDTGS
                     ARLNPGGLRVVDHLVEFMHAYPQRRVSIEGFTDSVGNPVSNQDLSERRASAVRLALID
                     RGVDPARLVARGYGEDYPVASNTNPAGRQLNRRVEIVISDANGMIGSRGSPPVANIGR
                     GPRG"
     gene            complement(15191..15775)
                     /locus_tag="VFO53_06370"
     CDS             complement(15191..15775)
                     /locus_tag="VFO53_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET8875937.1"
                     /translation="MKILLLCGAVVVTIAGSNVLAQTATDAKPPAKVDICAACHGADG
                     NSTNPQYPNLAQQTARYIYLQLKDFKEGRRKDPLMSPVAATLEQADMLALADWFSKQK
                     ENPTGFKADPAKVDAGRKKADEVLCSMCHGGDFVGQNEIPREAGQHYEYVKKQLLDFK
                     ARRRTNDAGNMTSVARTLSDEDIDNLAQFVANLH"
     gene            complement(15873..>16466)
                     /locus_tag="VFO53_06375"
     CDS             complement(15873..>16466)
                     /locus_tag="VFO53_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET8875938.1"
                     /translation="VHDRASGKLIRHSEPMIPHEGMYTLPTAQGARMLPGANGGVEWS
                     PMAVNPKLHMAYAANLHQPMTYHVEEAPYPGGSKLWLGGAFKTIPTEEQWGQLVGVNL
                     DNGKVMWKHKTPQPLIGGVLATAGDIVFNGEANGWYKAYDARTGKELWKFNCGAGVNS
                     PGVAYMVNGKQYIAVAAGGNNQIDAKRGNSVFVFALP"
BASE COUNT         2643 a         5586 c         5543 g         2694 t
ORIGIN      
        1 cgaaaagtcg tctctgaccc cacttttgcc acttttggac cccgaactcc gactcggact
       61 gcgcctcgtc cgcgggctca ccgaggcggg tgcgacacgc atcgttgatg cgcgtcgcga
      121 gcggccattc agcagcgtcg ccgacctcgc gcaccgcgcg cgtcttgacc ggcgcgacct
      181 gactgccttg gcggacgggg atgcgctcgc gtcgcttgcc ggtcaccgcc acgatgccgt
      241 gtggaacgtc gccggggtgg aacgcatgcc gccgcttctg gccggcagtc ccttcgatga
      301 agcgcaaccg gtgctgccgc cgcccaccga gggccaggac atcgtcgccg actatcgccg
      361 cctcggcctg acactgcgcc gccatccgct cgcgctgctg cgccgccagt tgcgcgcgcg
      421 ccggctcgcc accgcggacg acgtttgtgc cgcggcgcac ggccggctcg tgcgcaccgc
      481 aggcatcgtc atcggccgcc agcgccccga caccgcgagc ggcgtcgtct tcgtgacact
      541 cgaggacgaa accggcgcgt cgaacgtcat cgtgcggcgc gagatcggcg agcggcagcg
      601 gcgcgagctt ctcggcgcgc gactgctcgg cgtttatggc acggtggagc gcgaaggcga
      661 ggtcgtgcat atcctcgccg gccggctcgt cgacctgacg ccgatgctcg gcgcgttgcc
      721 cacgcgctcg cgcgacttcc attgacgcgg cagcaagcgc cggttgcacc attgtgcatc
      781 gcggctttga acggatcgac caaacgacca gtctgattct catcagaacc cgcaggatgc
      841 gccgcgtcgg cgtcaccggc tcgtcgcacc cgcgtcgcag gaacatcgtg aagagcgaat
      901 tcgatcttgc cgtcatcggg ggcggcgccg gcggtctcgt tgtcgccgcc ggcgccgcca
      961 agctcggcgc gaaagtcgcg ctgatcgaaa aggaccgcct cggcggcgac tgcctgtggc
     1021 acggctccgt cccgtcgaag acgctgcgca agtccgcccg cgtcgcgtac acgatgcgcc
     1081 acgccgaccg cttcgcgctc gcgccggccg atccgcggcc cgacctcgcg cgggtgatgg
     1141 agcacgttgc ggaggtcgtc gccggcatcg cgatgcagga cagccccgag catttcacga
     1201 gccttggcat cgacgtgatt catggcggcg gccggttcgt ctcgccgacc gcattcgagg
     1261 tcggcgggcg aatcatcacg gcccggcatt tcgtgctcgc gactggctcg cgccccgcga
     1321 ttccaccgat cgccgggctc gcgtacgtga cctacctcac gaatcagacg gtgttcgatc
     1381 tgcgcgagcc ggtgccgcgc ctggtcatcg tcggcgccgg accgaacggc agcgaaatgg
     1441 cgcaggcgtt ccgccgcctg ggcagcgacg tgatcgtcgt cgacatggcg aacaagatcc
     1501 tgccgcgcga ggacgccgat gtcgccgcgg tcgtacagcg ccagctggag gcggaaggcg
     1561 tccgctaccg cctcggcatt accgtcggcg gcgtgatgcc cggggatccg catgccggtc
     1621 gcatccggat gacgctgcgc acggcaaacg gcgccgtcga gcagctcgcc gccacgcacc
     1681 tgatgctggc cgcaggacgc atgcccaaca tcgagggtct tggtctcgat gcggcgggcg
     1741 tgcagctcga caacggccgc ctcgtcgtcg acgacgtgct cgcgacgacg aatccgcgca
     1801 tccgcgccgt cggcgacgtc gccggaacct ttccgtatac gcatgtcgcc gagcatcagg
     1861 cggacatcgt gctgcgccag acccttcaca ggatgagctg ggcgaagccg tcaccggtcg
     1921 ttccgtggtg cacgtatacc gacccggagc tcgcgcgcgt cggtctttcc gaaacgcagg
     1981 cgcgccgaca tggcatcgac tatcgcatct accgcttccc gttcgcgcag gtcgatcgcg
     2041 cgcgcgccga gggcgagacc gagggcttcg cgaagctcgt caccgaccgc gcgggccgcc
     2101 tgctcggtgc ggcgatcgtc ggcgccgacg ccggcgaact catcgccgag tgcgtgctcg
     2161 cgatcggcag gcggatgaag gtcgacgacc tatcggcggc gatccatgcc tatccgacgc
     2221 gctcggaagt cgtgttccgc gccgcggatg agcggcgtcg tcaggcggcc ggaggctcgt
     2281 cgcgggtccg gatgcgccgg ttgttcggcc tcggcgcgac gtgacgaacg cgcggcgcta
     2341 cgacgaacgc gcggcaacga tcctgccgcg cacctcgccg aagccgatcc ggtaatcctt
     2401 cgcctgcgcc cacccggtca ggatcaactc gtcgtcgtct tcgagaaacg tgcgctcgct
     2461 gccgtcgggc aggtgcagcg gccgcgcgcc gtttttggtg atctcgagca gcgagccgca
     2521 ggtgtcaggg gccgcgccgc tgatcgttcc cgagcccatc agatcgccga cccgcgtgtt
     2581 gcacccggaa acggtgtgat gcgcgagttg ctgcgcgatg ctccagtaca tcgtgcggaa
     2641 gttcgtgcgc gaaatcgtcg ccggcgcctt gccggccgcc gcgagcgccg cttcgagatg
     2701 gatgtcgtac gcctgcggcc ccgacgtcgc gaggtagggc aacggtggcg ggtcctgcac
     2761 cggcccatcg acgcggaacg gctccagcgc ttccatcgtc acgatccacg gcgagatcga
     2821 cgtggcgaac gtcttgctgt tgaacggacc gagcgggatg tactcccact gctgcagatc
     2881 gcgcgcgctc cagtcgttca tcagcaccat gccgaacacg ttcgacagtg ccgagcccac
     2941 cgggatcggc tcgccgagcg cgttaccgac gccaacgacg aatgccactt cgagctcgaa
     3001 atcgagccgc tgcgacggcc cgaacagcgg cagctgcgca tcgggacgtt tcgactggcc
     3061 gttcggccgc agcaccggcg tgccggacac gacgatcgag cttgcgcgcc cgttgtacgc
     3121 gatcggcatg cagagccagt tcggcagcaa cgcgtgcgcc ggatcgcgaa acatcgagcc
     3181 gacgttcgtc gcgtgctcct tcgacgagta gaaatcggtg tacgcgccga cggtgatcgg
     3241 cagcagcatc ttcgccgccg cctgcgaggc gaacgcgcgg gcgcgaagcc gtgcgtcatc
     3301 gcgcaggcgc ccgttttccg cgcgcaaaag atcgctgatc gcgatgcgcg cgcgccgcca
     3361 ggtgtcgggg ccacgctcga tgaacggatt cagcgtcggg cgattgaaca gccgctcgcc
     3421 cgggacgaga tccagcaacc ctgcctgctc gaggaccgac aggtcgagca cctgatcgcc
     3481 gatcgcaacg ccgacgcgcg gcgaatagtt cgcgggggtc gtgaagacgc cgtacggcag
     3541 gttctggatc gggaacggcg aatccttcgc gacggtgacg aacgacttct gcttcgcgtc
     3601 gccgggagtc atggtgcgtc gaagtggcgc gcaagaccgc gccagcacgc cgggtaatcg
     3661 cgttgcagct gaggtgacga gagtgcatac ggcgtggggt gaatcacgta gcggctctcg
     3721 aacatgaagg cgagcgtatc accgagccgc tgcggcgtga gctcggcatg cgaggcgcgc
     3781 tcgaaagttt cagcgtcggg cccgtgcccc gacatcgcgt tgtgcaggct gcccccgccc
     3841 ggcaggaatc cttccgactt ggcatcgtag gtgccgtgga tcagtcccat gtactcgctc
     3901 attgcattcc ggtgaaacca gggcggacga aacgtgtcct cggcgacgag ccagcgcggc
     3961 ggaaaggtca cgaaatcgac gttcgcggtg cccgccgtgt ccgacggcga cgtgagcacg
     4021 gtgaaaagcg acggatcggg gtgatcgaag ctcacggtat tgatcgcgtt gaagcgcgcg
     4081 aggtcgtatt tgtacggcgt gtaattgccg tgccacgcaa cgacgtcgag cggcgaatgg
     4141 tcgatcgggg cacgccacag ccggccggcg aatttcgcca cgagctcgaa atcgccttcg
     4201 ctttcactgt atgacgccac cggcgcgaga aagtcgcgcg gattcgcaag accgttggcg
     4261 ccgatggggc cgagctcggg cagccggaat tgcgcgccgt agttttcgca tacgtagccg
     4321 cgcgccggcg aacgctcgtc cgcgagctcg acggcgaagc gaaccccgcg cggcacgagc
     4381 gcgatctcgc cgggcgcgac gtcgagggtg ccgagctcgg ttgcaatacg caacgcgcct
     4441 tgctggggaa cgatcaacag ctcgccgtcg gcgtcgtaga catagcgccc gcgcatcgag
     4501 cggttgcacg caaagacgtg aatggcaatg ccgatctgcg cggccgcgtt gccgttgccc
     4561 gcgatcgtcg cgatgccgtc cacgaaatcg gccggcgcgt cgggcagcgg aaacggatcc
     4621 cagcgcagct gattcggcga tgtgtgcgta tcgtcgagcg gcgcggtggc gaagcgcggt
     4681 tgcgcgaacg gcgtgaaggc accgtgcagt acggccggac ggatgcggta gagccaggtg
     4741 cgccggttcg cgtgccgcgg cgccgtgaac gcggtgccgg acagttgctc ggcatagagc
     4801 ccgtagggtg gccgctgtgg cgaattgcgc ccgatcggca gcgcgcctgc gagcgcctcg
     4861 gtcgcgaatt cgctgccgaa ccccgattgg taccgtggat tcaccgcgtg ctgtcggctg
     4921 cttcgtcgag cacgctgcag gcgggcgtct gcgcgagcgc ggccttcgct gcggcgaccg
     4981 accggatatg gcccgcgtcg acgccgttct tgatcgccgt cacttcggcg aggatcgata
     5041 tcgcgatctc gggcggcgtg cggctgccga tgtagaggcc caccggtcca tgcaagcgcg
     5101 cgatctcgtc atcggacaga tcgaactcgc gcagccgctc gcggcgcttt tcgttgttcg
     5161 cgcgcgaacc gatggcgccg acatagaacg ccggcgactt cagcgcttcc atcagcgcga
     5221 ggtcgtcgag cttcggatcg tgcgtcagcg caatcaccgc cgagcgctcg tcgaggcgca
     5281 tcgcctgcac ggtgtcgtcg ggcatcgtgc gcacgaggcg cacgcccggc acgtcccagg
     5341 tctccgaata ttcctcgcgc ggatcgcaga tcgtcacgtc gtagccgagg cccaccgcga
     5401 tctgcgcaag atacttcgac aactgcgacg cgccgatcac gagcatccgg tagcgcgggc
     5461 catgaatcgt gcggagcacc tttccgtcga aagcgagacc gtcggaaggc tgtgccggat
     5521 gcagcgtcgc gaatccggac gcgagatcga gccggcgcgc cacgaggtga cccgtctcga
     5581 cttcgcgcaa cagcgcgcgc atgcccgatt cgcgcgtcag cggctcgagc acgagctcga
     5641 tggtgccgcc gcaaggcagg ccgaagcggc gcgcctcgtc ggccgacacg ccgtacgtga
     5701 cgacttcgca ccgcgtcgcg cgctggcctt cacgccgcga gcgatcgacg atgtcgtcct
     5761 cgatgcaacc accggaaacg gaaccgacga caagcccgtc ctcgcgcacg gcaagcatcg
     5821 cgccctccgg ccgcggcgag ctgccccagg ttttcaccac cgtcacgagc agcacgcggc
     5881 ggccgtcttc cacccacgcg gcgctgttgc gcagcacttc gagatcgacg ctatccatcg
     5941 tcactccacg acagtcacgg gcgatagccg cgcgcataca gcacgacgag cacgacgata
     6001 atcgcaacga tcgccgcata gcgaatccac tgcgagccgc cgggtggcgc aagcgatggc
     6061 ggcgccgcgg tctcggccat ggtctcctgc gccgctaccg tggcgcttgc gccgaccggc
     6121 gccggctcgt ccggcggcgc aaagcgcgcg acgaagcggg cgaaaaaatc gtcgctcatc
     6181 ttcgcggcaa cgccgttcac gagccgcgac ccgacctgcg caagcttgcc gccgatctgc
     6241 gctttcgcaa cataagtgag tgccgtctgc cgctcgcctt ccggcgcgag cgtgacacgc
     6301 gcgtcacccc gcgcgaaccc ggcggcgccg ccctgccctt cgaaattgag cgtgtaagcg
     6361 cgcggcgcat cgatgtcggt catcgtcatg cgtcctgtaa agcgcgcgga caccggacct
     6421 atgcgcgcgg acatcacgag ctcgtacgca tcctcgcccg tacgctcgag cgattcgcag
     6481 ccgggtacgc aggccttcag gacggcggga tcgttcagcg ccgcccagac cgcgaccggt
     6541 ggagccggaa cgatacgggt gttggacagt tccattcgcg aaagtgcgtc aggccgcgca
     6601 agcggtaccg gtcccgcggt gacgcggatg cgccagcgca tgcgcgaggt cggtcagcgt
     6661 ttccatattg tgcaccggaa ggaagtcgtc gacatgcggc agcaacgcgc gaatgccggc
     6721 cggccgcgcc tcgaagcccg catagcgcag aagcggattg agccacacga gccgcgcgca
     6781 cgacttggca agccgctccg cctcgaacgc gaggccctcg ccggcgtcgg cgtcgaggcc
     6841 gtcgctgatc agcagcacca cggcgttctg tccgagcagt cgccgcgacc atttacggtt
     6901 gaattccatg aggcacgtcc cgatgcgcgt gccgcccgcc cagtcctcga ccgcgctgga
     6961 aacgcgcgca agcgccacgt cgacgtcgcg atggcgcagg tgccgcgtga tgttcgtcag
     7021 ccgcgtgccg aaggtgagaa cctgcacgcg atggcgatcg ttggtgacgg catgcaggaa
     7081 gtgcaggagc atgcgcgcat agcgatccat cgagcccgac acatcgcaca ggacgcaaag
     7141 cggcggcggc cgccggcgcc gagtgcgaaa gcgcagcggc gtcaatgcgc cgcccgcacc
     7201 cacgctgcca cgcaacgtcg cacgcaggtc gatcgcatgc ccgcgcggcg cggcgatcgt
     7261 ccgtcgtacc cgccgtgcgg gcagcggcag gcgcatcctg cgcaacagcg ccttgacctc
     7321 ggcgagctcg tcgaccgtca tggtcgcgaa atccttgcgc tgcaacactt cgcgcgccga
     7381 aaacgacagc gccgcgtcga gctcggtttc ctgcgcatcg tcggtcgccg ttgccggcgg
     7441 gggcagcatc gcctgtgcaa gccgcgcggg aagatcgccg gcggcgtcgc tcacacgacc
     7501 ctggactttc ggcaacagcg ccgcgatcat cttctcgagc agccgcggat tgcgccagaa
     7561 catgtcgaat gcctgctcga agatcggcag gtgctcgcgc cgcgagacca gcaccgccgc
     7621 gagcgcttcg cggaagtcgc cgcggttgtc gacgccgacg gcgtcgatcg cagcgagcgc
     7681 gtcgatcacc ttcgccggtc ccacgggcag ccccgctctg cgtagcacgc gaacgaagtg
     7741 cagcacgttg tcggcgagat ggtggccccc ccacgcttgc ggcgggtcgg acgatcttcg
     7801 cgctccgcac tcagctgttc gctctcgggg cggcccgtcg cgctcacgcg ctgcgctgcc
     7861 cccggagggg gcgcaatgag cgcctcgggg cggcctttcg gcgctcatgt ccctaggccg
     7921 ccgcctgagc ttcacgcacg agcttcgcgg caacgtcggg cgtgatcgcg gcgacgtcgt
     7981 cctggtactt gagcaaggcg ccgagcgtgt cggcaaccgt ctgcggatcg agggtgatcc
     8041 ggtcgagtgc gacgagcgct tccgcccagt cgagcgtctc ggcgatgccc ggcgccttga
     8101 acagatcaag cgtgcgcagc cgctgcgtga acgcgacgac ctcgcgcgcc agcgatgcgg
     8161 acgccgccgg acatttgcgc gcgacgatcg caagctcgcg cgccgcgtcg ggatagtcga
     8221 cccagtggta aaggcagcgc cgcttgatcg cgtcgtggat ctcgcgcgtg cgattggacg
     8281 tgatcacgac gacgggcggg gtttgcgcgc gaatggtgcc gagctcggga atggtcacct
     8341 ggtaatcgga gagcacttcc agcaggtacg cctcgaacgg ctcgtccgcg cgatcgagct
     8401 cgtcgatcag cagcacgggt ggcgcgccgt cttccggcga gagtgcctgc aggagcggac
     8461 ggcgcagcag aaaacgcgtg gtgaagatgt cgtgcgaaag ctcgccccgc tcgacgccac
     8521 ccgcggcttc cgcaagccgg atctcgatca tctgccgcgg atagttccac tcgtatacgg
     8581 ccgccgacgt gtcgaggcct tcgtagcact gcagccgcac cagcggtcgc gcgagacccg
     8641 cggcgagcac cttggcgatt tcggtcttgc ccacacccgc ctcgccctcg aggaagagcg
     8701 gccgctgcag cttcaatgcg aggaagaccg cggtcgcaag ccggcgctcg gcgacatagt
     8761 cgtgctgctc gagcagcgcg agcgttgcgt cgacgttgaa cggcgcttgc aattgcgttt
     8821 gcgttcgttg ctgattcacg atcatgccga tcgaagcgtc aggttcgatt ctacggcaga
     8881 gttatttgcg tgcaaggtag agcgtccgtc gggcgatcgt ccatctgcgg gccgccacgc
     8941 cgccgccatt cagacggaga gtttctcctc cagccagatc tttatcaacg actgataggg
     9001 aacgtcacgc gcattcgcgg ccgccttgat tcgctcgagc atgtggttcg gaaggcgcag
     9061 cgagatgctt tgcgtcgtcg gcttgagatt gggaagattg acgcggcgag cctgcgacca
     9121 atcgagcacc cgggaactgt cgtggctttc ccaataggcg cgctccgcgg cctctgattc
     9181 gaattgcgga attctagacg gcctgctcat ggatcgccct ttcctttcga tgcatgtcgc
     9241 gtgccgcgat cacgcggatc agcgtcgcgc catcgcgaag cgtgaatgta atatgcaaat
     9301 gacggccctc gcgcgtcatg cccaatgcgt gatagcgtgg ctcccgcagc gaatgttgtg
     9361 tgccttccat gatcagcagt cgcgtgtcga aaaacgcctg ctccgcctcg ccttgcgaaa
     9421 cgccgtgccg ctcgttcttg cgtgcgttgc cgtcatccca ttggaagccg gcgatcgcgt
     9481 cgaagtcgat cacattcgtg tattaccata atatacgctg ccctagaggg aatcgacgtc
     9541 aggcgtcgaa cgggctctgc cattgcaatc tcgcctagcg gctgcggctg ccgctgcggc
     9601 agtaggaccg gatttaccga atggtgcgtg aaaacgggcg gcatcgggcc gcccgttcac
     9661 gcgcaagcgg tggccgcgtt acgcgatggc cgccacggcc cgcgacgcca tcaccgaaac
     9721 cagatgcgcg cgatattcgg ccgacgcgtg caggtcgttg ttgaggccgc tcgcatcgat
     9781 cgcgacgccc ttcgccgcaa ccggcgtgaa actccgcgcg agcgcactct cgatgcccgt
     9841 tgcccggaac accgacggcg ccgcgcccgt gacggcgacg cgaacgtcac cgttcgacga
     9901 ccgcgcgacg aacacgccca ccagcgcgaa gcgcgacgcg ggattcggga acttgacgta
     9961 cgccgcacgc tcgacgcgcg gaaaatcgac cgcggtgatg atctcgtcgg gcccgagcgc
    10021 cgtttcgaac atccccttga agaagtcgtc ggccgcgatg gcgcgctggt gtgtccggat
    10081 cgtggcgccg gtcgcgagca cggcggccgg ccagtcggcg gcgggatcgt tgtttgcaag
    10141 cgagcctccg agcgtgccca tgttgcgcac ctgccgatcg ccgatgccct cggcaagatc
    10201 gcacagtgcc ggcatggccg tcctcaccag gtcggatgac gcgatttcgg cgtgcctcgt
    10261 catcgcgccg atcacgaggc gctcgccgtc cctgcggatg ccgcgaagat cggcgatgcc
    10321 ggcgagatcg acgagcgtgc cgggctgcga cagccgcaat ttcatcgcgg cgacgaggct
    10381 ctggccgccg gcgagcgggc gcccgccggt cgacgccagg accgacaccg cgtcggcgag
    10441 cgtttttggt tgctgatagt cgattgcgta catgagcgtt ccttatgcgt gtccgcgtgc
    10501 ctgccggatt gcgccccaca cccgatgcgg cgaggccggc atgtcgagat gcgcaacgcc
    10561 gagcggcgcg agcgcatcca tgacggcatt catcatcgcg gccggtgcgc cgatggcgcc
    10621 cgcttcgccg caacccttga cgcccagcgg attgtgcgtg cacggcgtca ccttcgtgcc
    10681 gacgttgaac gacggcagat cgtcggcgcg cggcagcgcg tagtccatca tgctgccggt
    10741 cagcagttgc ccgctgtcgg gatcgtagac gcagtgctcg agcagcgcct ggccgaggcc
    10801 ctgcgcaaca ccgccgtgga cctggccttc gacgatcatc ggattgatga tgttgccgaa
    10861 gtcgtcgcac gcggtgaacg cgacgacgtc gacgacgccg gtatcgggat cgatctcgac
    10921 ctcgcagatg tgcgtgcccg ccggatacgt gaagttcgtc gggtcgtaga acgccgtctc
    10981 gttcaacccg ggctcgaggg tcgcgagcgg atagttgtgc ggcacgtagg cggtcagcgc
    11041 gatctcgccg aacgtcttgc tgcgatcggt gcccgcgacc gtgaacttgc cgtccctgaa
    11101 ctcgatgtcg gattcggcgg cttcgagcag atgcgcggcg atcttctttc ccttcgcgac
    11161 gatcttgtcg agcgccttca cgatggcgct gccgccgacg gcgagcgagc gcgacccgta
    11221 ggtgcccatg ccgaacggca cgcgccccgt gtcgccgtgc acgacgctca cgttgtcgac
    11281 cggcacgcca agatgcgcgg cgacgacctg cgcgaacgtc gtctcgtgtc cctggccgtg
    11341 gctgtgcgag ccggtgaaca ccgtgacgct tcccgtggga tgcacgcgca cctcgcccgc
    11401 ctcgaacagg ccggcgcgcg cgccgagcga tcccgcgatg ttggacggcg caagcccgca
    11461 ggcttcgatg tacgcggcgt aaccgagtcc gcgcagcttc ccccgcttct gcgcctcggc
    11521 acgccgtgcg gcaaagcccg cgacgtcggc catgtcgatc gcagcgtcga gcgtctggtc
    11581 gtaattgccg gtgtcgtagt tgagcgcaac gggcgtctga tggggaaagt cgcgaatgaa
    11641 attgcgccgg cggatctcgt cctgcgcgat gcccatctcg gccgccgcct gatggacgat
    11701 gcgctcgacg acgtacgtcg cctcgggccg tcccgcaccg cggtaggcgt cgaccggcgc
    11761 cgtattcgta aacgcggcgg tcacctcgca atggatcgcc ggcgtcgtgt actggccggc
    11821 aagcagcgtc gcatagagga tcgtcgggat gcaggacgcg aacgtcgaca gatacgcgcc
    11881 catgttcgcc gtggtttgca cgcgcagcgc gaggaacctg ccatccttgt cgagcgccag
    11941 ctcggcgtgc gtcacgtgat cgcggccgtg ggcgtcggac aggaacgatt cgctgcgctc
    12001 ggccgcccac ttgatcggcc ggttgatgcg cttcgacgcg aacaccatcg ccgtctcttc
    12061 gggatacagg tagattttcg agccgaatcc gccgccgacg tcgggcgcga tcacgcgcac
    12121 cttgtgctcg ggcaggccga gcacgaacgc ggtcatcagc agccgctcga cgtgcggatt
    12181 ctggctcgcg acataaagcg tgtacgcatc gtcggcgcgg tcgtacgacg caactgcggc
    12241 gcgcggctcg atcgcgttcg ggataaggcg attgttgacg atgtcgatct tcgtcacgtg
    12301 tgccgcgccg gcgaatgcgg cgtcgaccgc cgccttgtcg ccgatcgccc acgtgtagca
    12361 cttgttgccg ggcgcctgct cgtggatctg cggcgcgcca ggcgcaagtg ccgcgaccgg
    12421 attgacgacg gccggcagca cgtcgtagtc gacgacgatc tgctcggccg cgtccttcgc
    12481 ctcggcgatc gattcggcaa tcaccagcgc gacggcatcg ccgacatacc ggaccttccc
    12541 ctgggccagc accggatgcg gcggctcgtt catcggctta ccgtcggtgc cggtgatgag
    12601 ccagccgcag ggcagcccgt tcactccggt caggtcggcg ccggtgtaga tctcgacgac
    12661 gccgggcaac gtcttcgcct tcacgacgtc gatcgagcga atgaccgcgt gcgcatgcgg
    12721 agagcgcagg aaacaggcga aggtctggcg cggcatcgac acgtcgccgg tgtattggcc
    12781 ggtgccggtg agaaagcgcg catcttcctt gcgcagcacc ggctgcccga tgacgctcgc
    12841 gagggtcgtg gtgtcgttcg tacccatcgt cgtctcctat ttgcccatga ccacagccgc
    12901 ctgccgcacg gctttcacga tgttgtgata accggtgcag cggcacaggt tgccttcgag
    12961 gccggcgcgt atcgtcgctt cgtcggcgcc gggatgccgg ttcacgagat cgatggcgct
    13021 catcaccatg cccggcgtgc agaagccgca ctgcaggccg tggcagtcgc gaaacgccga
    13081 ctgcatcgga tgcatcgtgc cgtcctcggc aatgaggccc tcgatcgtga cgaccgacgc
    13141 accttgcgcc tgcacggcaa gcacgttgca cgacttcacc gcctggccat cgagatgcac
    13201 ggtgcacgcg ccgcattgcg cggtgtcgca accgacgtgg gtgccggtga gcgcgagatt
    13261 gttgcgcagg aagtcgacga gcagcgtgcg cgcgtcgacg tcggcgcgca ccggcttgcc
    13321 gttgactgac atttcaagcg gaacggacac gttgtcctcc ttcggcgaat gacgggcgcg
    13381 ggatgcgttt ttcgttgtcg cgcggggcaa caagcatact cccgcgcacg ctcgccatgg
    13441 aagaggcgcg tgtcgcgcaa aagtgcaatg ccgcgcgccc gcgtcggcgt gcatcttgct
    13501 cgaattcgac cgcaccccgc gcagcgggcc gcgtttgtaa ttcctgcctt gcggccgttg
    13561 cagcatcggt aacgatgtcg gaacgcgacc ggacattggc gggttccgcg tccgacgagg
    13621 agatcaacat gcttcatgaa agattcaaac gcacgatcgg catcgcgggc gcagcatcgg
    13681 tcgcgatatt ggcggccggc tgcgtcaccg tcgcaacgcc gtcgccgacc ggaaggctcg
    13741 ccgtcgcgaa tgcggcgatc gccgacgcga tcagcgccga cgcgtcgcaa tacgacgctg
    13801 ccgatctcga caaggcgcgc agaaagctcg cccgcgcgca cgacgaggct gcgcagggcg
    13861 actatggcgt ggcacgggat ctcgccgagg aggccgaggt cgatgcccgc ctcgccgcaa
    13921 cgcgcgcgcg ctcgacgaag gccgcccacg ccgccgcggc cgtgcaggaa agcatccgtg
    13981 cgctcgacga cgagatcgcg cgcacaccgc attgaaggag tgacaccatg aaagcctgga
    14041 aagccagaat gggaatcgtc gtgccggcat tcgccctcgg cgcctgcgcg gtgctgcctg
    14101 cccacgacgc ggaagtcgac gatgcacgcg ccgccgtgct cgctgcgcag aacgacccgc
    14161 aggtcgtctc gctcgccccg gtcgaattga acaaggcggt cgatgcgatg cgtcaagccg
    14221 atgccgcatg gtcgaaccgc ggcagcatcg aggaggtgca ccacctcgcg tacctcgcat
    14281 ggacgcacgc ggcgatcgcg accgaaactg cgcgactcaa gacggcggag gcatcggtcg
    14341 cgtccgccaa cgcggagcgc gaccgcgtgc gtctcgcggc gcgcacgcgc gatgccgagc
    14401 gcgcacagcg ccaggcggcg cgcgcgcagc aggacgcagc ccgcgcgcag gcgcaagccg
    14461 acgcaaaccg cgcgaacgcg cttgccgcgc agcagcaagc cgccgatgcc gcacgtcagg
    14521 cgcaggcggc gcgcgatcag gccgaggcgt cgcgcgcgca ggcggccgac gcgcaggcgc
    14581 gcgcacagtc gctgcaatcg tcgcttgccg agttgcaggc gcgcccgacg gaccgcggca
    14641 tggtcgtgac catgggcgac ctcttgttcg acaccggcag cgcccgcctc aatccgggtg
    14701 gccttcgcgt ggttgaccat ctggtcgagt tcatgcatgc gtatccgcaa cggcgcgtga
    14761 gcatcgaggg gttcaccgac agcgtcggca atccggtgtc gaatcaggac ctgtcggagc
    14821 ggcgcgcatc ggccgtgcgg ctcgcgctga tcgatcgcgg cgtggacccg gcgcggctgg
    14881 tggcgcgcgg ttacggtgag gactacccgg tcgcgagcaa taccaatccg gcgggacggc
    14941 agctcaaccg tcgcgtcgag atcgtgatct ccgacgcgaa cggcatgatc ggatcgcgcg
    15001 gatcgccgcc tgtcgcgaac atcggacgcg gaccgcgcgg ctgagcttcg gggtcagagc
    15061 ttcggggtca gagctgtaat tatcgcggcg cgactgagct tcggggtcag agctgtaatt
    15121 attgcggcgt cggccgcggc gtccgcgcgt aaccgttcgc gccgcaataa ttacagctgt
    15181 gaccccgaag tcaatgcagg ttcgcgacga actgcgccag attgtcgatg tcctcgtccg
    15241 acagcgtgcg cgccacgctg gtcatgttcc cggcgtcgtt ggtgcgccgc ctcgccttga
    15301 agtcgaggag ttgcttcttc acgtattcgt agtgctgccc cgcctcgcgc ggaatttcgt
    15361 tctgcccgac gaaatcgccg ccatggcaca tcgagcagag cacctcgtcg gccttcttgc
    15421 gtcccgcgtc gaccttcgcc ggatcggcct tgaacccggt gggattctcc ttctgcttcg
    15481 agaaccagtc ggcgagcgcc agcatgtcgg cctgctcgag cgtcgcggcc accgggctca
    15541 tcagcgggtc cttgcgccgg ccttccttga agtccttgag ctgcaggtag atgtagcgcg
    15601 ccgtttgctg cgcgaggttc gggtactgcg gattcgtcga attgccgtcg gcgccatggc
    15661 aggcggcaca gatgtcgacc ttcgccggcg gcttcgcatc ggtcgccgtt tgcgccagca
    15721 cgttcgatcc ggcgatcgtc acgacgacgg cgccgcaaag cagcaggatt ttcatcgcaa
    15781 tgagggagcg ccggcgccag atgccggaat cgaggtggac atgaaaacgg ccgcaccggt
    15841 tccggcgcgg ccgttcgcga attgctttcc gattacggta gcgcgaacac gaacacgctg
    15901 ttgccgcgct tcgcatcgat ctggttgttc ccgccggccg cgacggcgat gtactgcttg
    15961 ccgttcacca tgtacgcaac gccgggcgag ttgacaccgg cgccgcagtt gaacttccac
    16021 agctccttgc cggtgcgcgc gtcgtacgcc ttgtaccagc cgttcgcctc gccgttgaac
    16081 acgatgtcgc cggccgttgc gagcacgccg ccgatcagcg gctgcggcgt cttgtgcttc
    16141 cacatgacct tgccgttgtc gagattgacg ccgaccaatt gtccccactg ctcttcggtc
    16201 gggatcgtct tgaacgcgcc gccgagccac agcttgctgc cgcccggata cggtgcttcc
    16261 tcgacgtgat aggtcatcgg ctggtgcagg ttcgccgcgt acgccatgtg cagcttcgga
    16321 ttgaccgcca tcggcgacca ctcgacgccg ccgttcgcgc ccggcagcat gcgcgcgccc
    16381 tgcgccgtgg gcagcgtgta catgccctcg tgcgggatca tcggctcgga gtggcggatc
    16441 agcttgccgc tcgcgcgatc gtgcac
//
LOCUS       DASSVX010000146        10857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_655441, whole genome shotgun sequence.
ACCESSION   DASSVX010000146 DASSVX010000000
VERSION     DASSVX010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10857
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655441"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFO53_06380"
     CDS             <1..470
                     /locus_tag="VFO53_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET8875939.1"
                     /translation="AITFWLMGSLASASAAEVASLLPAVALALVVLFALAWRVNLLTL
                     PEDEARALGVSTRRLRGVVIAAATLATAASVAVSGVIGWVGLVVPHMARLVVGPEFSR
                     LLPAAALFGAAFMLVIDTISRTVAPIEIPPGILTAVIGTPVFIALLARAQRIF"
     gene            475..1278
                     /locus_tag="VFO53_06385"
     CDS             475..1278
                     /locus_tag="VFO53_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8875940.1"
                     /translation="MSRMANATQARPPILDVRDLAFGFGTRRVGEGVTFALAAGETLA
                     VLGGNGAGKTTLLRTLLGLLPARQGSVTLEGDPLAQLAPSQRARRVAYVPQHSAPAFA
                     FTVLEAVVMGRAAHLGTFGRPRHDDYASAQRALATLGIDALAQRRVTEISGGERQLVM
                     IARSLAQHARVLVLDEPTASLDFGNRVRVLGEIDRLRADGMTILFSTHEPDQALAHAD
                     RALLLAGGRVIALDTVERALTTENIERLYGTPVRLVSFDGHRYACVPRD"
     gene            1539..1745
                     /locus_tag="VFO53_06390"
     CDS             1539..1745
                     /locus_tag="VFO53_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875941.1"
                     /translation="MGTSLATHTESTFKPSGVASKALEEFKARRAAEKLGLLPPAKSK
                     VVERYEIDTDTATVTKLPLRMKTR"
     gene            complement(1750..2670)
                     /locus_tag="VFO53_06395"
     CDS             complement(1750..2670)
                     /locus_tag="VFO53_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8875942.1"
                     /translation="MAKAQSPAPQGVSGPAEMMDTREVAAYLRLKERRIYDLVRQRAI
                     PHVRATGKLLFPRAQIDAWIAGKREAPAAMPRARAPIIAGSHDPLLEWAARESRCGLA
                     ILAGGSRAGVEALARGEATAAATHWLDEASGEYNVPLVRELFSGADVVVLEWARRTQG
                     LLLREGNPHRIRKLADLARKHVRIASRQPGAGSHRLLLHLLAQAGIGADALDWSPRIA
                     HAETELAAIVRDGHADVGLGIEAGARANGLAFVPLAVERFDLVTFRRDAFEPPLQALL
                     ACTRTPEFAATAASLRGYDVTLTGRVVFNA"
     gene            complement(2682..3938)
                     /locus_tag="VFO53_06400"
     CDS             complement(2682..3938)
                     /locus_tag="VFO53_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875943.1"
                     /translation="MKAGSETTFQRGSAARAAGAGKSSLTLLSCARTLAILRKELLDT
                     FRDRRTGTVTLLSSILAGPIFLMLIFNLMASQAERARELTLPVQGGEYAPAVVAYLQR
                     QQVKVTKAPDDYDAQIRRGDLDVVLIIDDAFANDVAQGKPATVRLVFDRSRDRARASI
                     DQADALLRNYNRLWGEERMMLRGLTSSVANPLRIESDDVATPQQSGALVLFLVAYYGL
                     FASVMGGMAVALDTTAGERERQSLEPLLMTPARPSEIVVGKWLAVCLFDALVVTLTLT
                     GFYLTLAFAPLPPVGVPFLFGAREFGRFLLVLVPMILMLPAILLYVGSRARAYKEAQA
                     NVSVLLFVVSLIPLVQLFMQRKEPSWIMLVPVSAQYSLLNTSLRGEAPALAQLALSWL
                     APLALVAIALVAVSRRLSRETILSGK"
     gene            complement(3935..4678)
                     /locus_tag="VFO53_06405"
     CDS             complement(3935..4678)
                     /locus_tag="VFO53_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8875944.1"
                     /translation="MIAVRDIAKAFGKRRDIRAVRDVTFDAPDGAITGLLGPNGAGKT
                     TLLRMIATLVIPDEGHANVDGLDVVADRYAVRARIGVLSDARGLYPRLTARENVRYFG
                     ALHGLSGASLDARIDTLFATLGLGAIAERRTAGFSQGEKMKVAIARALVHDPHTILLD
                     EPTNGLDIMSVRTLRDELRALRSAGKCLLFSSHVMQEVAALCDRIVVLSQGRVVAAGT
                     ADELIARAGTAGLEDAFVALLGTDEGLAA"
     gene            complement(4675..6150)
                     /locus_tag="VFO53_06410"
     CDS             complement(4675..6150)
                     /locus_tag="VFO53_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8875945.1"
                     /translation="MTRTLRLCGVVGLLGCLLLVAGCSDRAPATRRLTLAECRLPKVA
                     QAVQCGTLEVPENRRQPNGRRLSIFVALLPANTLSPKPDPLVLLAGGPGQAASTLGPF
                     ALSLTAIRRTRDIVLIDQRGTGRSSPLACPAFAPDEHAEFDTDPVPKALLCAWQLAGK
                     GVDASQYTTSAWVADLDEMREALGYDKLNLWGGSYGTRVAQEYLRRHPSHVRSVVLDG
                     VAPPSLRISFDVWRTRDDALDGVIAACRASKPCAKAHPDPAATLEDIRRALEGGRTVS
                     VRDPRTGVPREIRAEFDTIIGALQPLTYSPEAASLIPELLALAHDGQYAPLLAASLTV
                     VGDLPYEFNPALFYSVTCSEDAPRVTRDERANGVADVRVRSLARRALAVCDQWPKGGY
                     PVDFTMPVQSALPVLLLSGGLDPVTPPVYGDEVAKGLPNSRHVIAAGFGHIVSPHACA
                     PRLIAAFVDAAGFDTLSQDCIDFFAHSKRPPSWPDRLAPQP"
     gene            6241..7092
                     /locus_tag="VFO53_06415"
     CDS             6241..7092
                     /locus_tag="VFO53_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019453009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HET8875946.1"
                     /translation="MAELEPGIVTDLRDRQSYGGYLRLDRLLDAQQPVSGGADETPRH
                     DEMLFIIQHQVSELWMKLMIHELRAAIAHVRADALEPCFKILARVKLIQKQLFEQWAV
                     LETLTPSEYEAFRPALGTASGFQSAQYRAIEFLLGNKHAAILDVFRHEPATFAELDAL
                     LHAPSLYDEFLRLLARRGLPMPNASLARDVTKPYVRNPELVPVLARIYADPEQWWDAY
                     DMCEKLVDVEEAFQLWRFRHMKTVERIIGYKPGTGGSSGVAFLRRALEHSFFPELIDV
                     RTVIGAR"
     gene            7089..8309
                     /locus_tag="VFO53_06420"
     CDS             7089..8309
                     /locus_tag="VFO53_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET8875947.1"
                     /translation="MSSGPIEAAVAALGNAPLTEAALAAHIAPLFSRMREAAPDRVYL
                     ANHSLGRPLDAMEHDVREGLAAWYARMGGAWDAWMDEMQAYRMRLARLMHAPRSDCVV
                     PKTSAGQGLRAILNTYDRAARPRVVATRGEFDSLDVILREYARRGRIELALVEPRAGG
                     SFDAADIADAIGNRVDLVVVSEVIFNTGQRIGRIDAIVERTHAAGGRVLLDVYHSLGV
                     VPVDVTAQDVDFAVGGSYKYLRGGPGACFLYLHPRHLDGALLRTLDIGWFAKREPFAY
                     ARPDPPELAPGGDAFLESTPPALTWYQARAGQRLALALDVDRVRAYSLQQQQTLVTLL
                     RARGIGAQGGSPDHGAFVVVTDPRAQSWCATLATRGIVADARGERLRLCPDVLTTEEQ
                     LVRAAEALGAIARR"
     gene            complement(8352..8897)
                     /locus_tag="VFO53_06425"
     CDS             complement(8352..8897)
                     /locus_tag="VFO53_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HET8875948.1"
                     /translation="MQRMRSIAAAGAAAAGLALMVLPAAGAAADEFSQSQIQRGFEIN
                     PVPLDLRGKNRALVGLGSYIVNSGGCSECHTNPSFAPGGNPFFGEPEQVNTAGYLAGG
                     QHFGPFTSANITPDALGRPAGLTLEQFISTLRTGHNPLDPPGTLLQVMPWPSFGKKTD
                     RDLTAIYEYLRSIPSIENDNN"
     gene            complement(9017..9271)
                     /locus_tag="VFO53_06430"
     CDS             complement(9017..9271)
                     /locus_tag="VFO53_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HET8875949.1"
                     /translation="MTEPPRTPPPAQDPATTPPPPRQATLRQIVGAVFWSFFGVRTGD
                     AMRRDIVTIKPHQVIIVGILIAAGLVVALLILVRIITRNA"
     gene            9429..>10857
                     /locus_tag="VFO53_06435"
     CDS             9429..>10857
                     /locus_tag="VFO53_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET8875950.1"
                     /translation="MRFDKLTTKFQQALADAQSLALGHDNGFIEPQHLLAALLAQEDG
                     GTASLLARAGVNVPRVQAGLRTEINNLPKVSGTGGEITISRDLNNLLNLTDKEATRRG
                     DQFIASELFLLALTDDRGPTGRLLKDNGLTRASLEAAIDAVRGGGNVDSQEAEGQREA
                     LAKYTLDLTERAREGKLDPVIGRDDEIRRTIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPETLKNKRVLSLDMAQLLAGAKYRGEFEERLKAVLKDIAADAGRTIVF
                     IDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQ
                     KVLVDEPSVESTIAILRGLQERYEIHHGVDITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAAARIKMEIDSKPEAMDRLDRRMIQLKIEREAVKKEKDEASQRRLAALEDEIGR
                     LEREYADLEEVWKAEKAQVQGSQHVK"
BASE COUNT         1767 a         3693 c         3759 g         1638 t
ORIGIN      
        1 ccgcgatcac cttctggctg atgggaagcc ttgcatccgc gtccgcggca gaggtcgcga
       61 gcctgctgcc ggccgtagcg ctcgcgctcg tcgtgctgtt cgcgctcgcg tggcgggtca
      121 acctgctcac gttgccggag gacgaggcgc gggcgctcgg cgtgagcacg aggcgattgc
      181 gcggcgtcgt catcgcggcg gcgacgctcg cgaccgcggc aagcgtcgcc gtttccggcg
      241 tcatcggctg ggtgggtctc gtggtgccgc acatggcgcg tcttgtcgtc gggcccgagt
      301 tctcgcgcct gctgccggcc gcggcgctgt tcggcgccgc cttcatgctc gtcatcgaca
      361 cgatttcacg taccgtggcc cccatcgaga tcccccccgg gatcctgacg gccgtgatcg
      421 gcacgccggt gttcattgcg ctgcttgcac gcgcgcagcg gattttctag gccgatgtcg
      481 cgaatggcga acgcgacgca agcacgcccg ccgatcctcg acgtgcgcga cctcgccttc
      541 ggcttcggca cgcgccgtgt cggcgagggc gtgacgttcg cgctcgcggc cggcgagacg
      601 ctggcggtgc tcggcggaaa cggcgccggc aagacgacgc tgttgcgcac gctgctcggc
      661 ctgctcccgg cccggcaggg cagcgtgacg ctggaaggcg acccgttggc gcaacttgcg
      721 ccgtcacagc gcgcgcgacg cgtcgcctac gtgccgcagc atagcgcccc cgcattcgcc
      781 ttcaccgtgc tcgaggccgt ggtgatgggc cgcgcggcgc acctcggaac gttcggccgc
      841 ccaaggcacg acgattacgc gagcgcgcag cgcgcgctgg caacgcttgg catcgacgca
      901 ctggcacagc gccgggtcac cgagatctcg ggcggcgagc gccagctcgt catgattgcg
      961 cgctcgcttg cgcagcacgc gcgcgtgctg gtgctcgacg agcccacggc aagcctcgac
     1021 ttcggcaatc gcgtgcgcgt cctcggcgaa atcgatcgcc tgcgcgcaga tggcatgacg
     1081 atactgtttt cgacgcacga gccggatcag gcgctcgccc acgccgatcg cgcgctgttg
     1141 ctcgcgggcg ggcgggtgat cgcgctcgat acggtcgagc gcgcgctcac gaccgaaaac
     1201 atcgagcggc tctatggcac gccggtgcgt ctcgtcagct tcgacgggca ccggtatgcc
     1261 tgcgtgccgc gcgactgaac aacgtgtacg ccacattgcg gacgagtgcg cgatgtgcgc
     1321 gaaagcgaca cgctcgcgcc accgcgggcc tacgctcgtc gcccgccgcc ccatcgccgt
     1381 ttgcagcgtg catacttatg acgccggcat acacagcgtc gaacgccggt gaatccgtca
     1441 gggcgcgaaa cgcgtcaacg cacactccgg tatcgaccat ggcctcgggc aaatcgagca
     1501 gcatccacaa acgcaagctg gcagcggcgc tccgcaaaat gggcacgtcg ctcgcgacgc
     1561 acaccgaatc cacgttcaag ccctcgggcg tggcctcgaa ggcgctcgag gaattcaagg
     1621 cacgccgcgc ggcggagaag ctcggtctgc tgccgccggc gaagtcgaag gtcgtcgagc
     1681 gctacgagat cgacaccgac acggcgaccg tgaccaagtt gccgctgcgc atgaagacgc
     1741 gctgacgaat cacgcgttga agacgacgcg gccggtgagc gtcacgtcgt agccgcgaag
     1801 cgatgcggcg gtcgcggcga actccggtgt gcgcgtgcag gcgagcagcg cctgcagcgg
     1861 cggctcgaac gcatcgcggc ggaacgtgac gagatcgaag cgctcgaccg cgagtggaac
     1921 gaaggcgagg ccgttggcgc gcgcgcccgc ttcgatgccg aggccgacgt cggcgtggcc
     1981 gtcgcgcacg atcgctgcga gctcggtctc cgcgtgcgca atgcgcggcg accagtcgag
     2041 cgcatccgcc ccgatgccgg cctgtgccag cagatgcagc agcagccggt ggctgcccgc
     2101 gcccggttgc cgcgacgcga tgcgcacatg ctttcgcgcg aggtccgcga gcttgcggat
     2161 gcggtgcggg ttgccttccc gcagcagcag gccctgcgtg cggcgtgccc attcgagcac
     2221 aaccacgtcg gcaccggaga aaagctcgcg caccagcggc acgttgtatt cgccgctcgc
     2281 ctcgtcgagc cagtgggtgg ccgccgcggt tgcttcgccg cgtgcgagcg cctcgacgcc
     2341 ggcgcggctg ccgcccgcga ggatggcgag gccgcagcgc gattcgcgcg ccgcccattc
     2401 gagcagcggg tcgtgactgc ccgcgatgat gggggcgcgt gcgcgcggca tcgcggccgg
     2461 cgcttcacgc ttgcccgcaa tccacgcgtc gatctgggcg cgcggaaaca gcagctttcc
     2521 ggtggcgcgc acgtgcggga tggcgcgctg gcgcacgaga tcgtagatgc gccgctcctt
     2581 gagccgcagg taggcggcga cctcgcgcgt gtccatcatt tcggcgggcc cggaaacccc
     2641 ttgcggtgca ggcgattggg ccttggccac gaccgattct ctcacttgcc gctcaggatc
     2701 gtttcgcgcg acagccgccg cgaaaccgcc acgagcgcga tcgcgaccag agcgagcggg
     2761 gcgagccacg acagcgcgag ctgcgcgagc gccggcgcct cgccgcgcag cgacgtgttg
     2821 agcagcgagt attgcgccga caccggcacc agcatgatcc acgacggctc cttgcgctgc
     2881 atgaagagct ggaccagcgg gatcagcgac acgacgaaca gcagcaccga cacgttcgcc
     2941 tgcgcctcct tgtacgcacg ggcgcggctg ccgacataga gcaggatcgc gggcagcatc
     3001 aggatcatcg gcacgaggac cagcaggaag cggccgaatt cgcgtgcgcc gaacaggaac
     3061 ggcacgccga ccggcggcag cggcgcgaac gcgagcgtca ggtagaagcc ggtcagcgtc
     3121 agcgtcacca cgagcgcatc gaacaggcac actgcgagcc acttgccgac gacgatttcc
     3181 gacggccgcg ccggcgtcat cagcagcggc tcgagcgact gccgctcgcg ctcgcccgcc
     3241 gtcgtgtcga gcgcgacggc catcccgccc atcaccgacg cgaacaggcc gtagtacgcg
     3301 acgaggaaca gcacgagcgc gcccgactgc tgcggcgtcg cgacgtcgtc gctttcgatg
     3361 cgcagcgggt tggcgacgct cgaggtcagg ccgcgcagca tcatccgctc ttcgccccat
     3421 agccggttgt agttgcgcag cagcgcatcg gcctggtcga tcgacgcgcg tgcgcggtca
     3481 cgtgagcggt cgaacacgag gcgcacggtg gcaggcttgc cctgtgccac gtcgttggcg
     3541 aacgcatcgt cgatgatcag cacgacgtcg agatcgccac gccggatctg cgcgtcgtag
     3601 tcgtccggcg ccttggtcac cttgacctgc tgccgctgga ggtaggcgac gacggccggt
     3661 gcgtactcgc caccctgcac cggcaacgtg agctcgcgcg cacgttcggc ctggctcgcc
     3721 atcaggttga agatcagcat caggaagatc gggccggcca ggatcgacga cagcagcgtc
     3781 accgtgccgg tgcgccggtc gcgaaacgta tcgagcaatt ccttgcgcag gatggcgagc
     3841 gtccgcgcgc acgaaagcag ggtcagagac gactttcccg ctccggccgc gcgggcggcc
     3901 gagccgcgct ggaaagtcgt ctctgaccct gctttcatgc ggcgagccct tcgtccgtcc
     3961 cgagcagcgc gacgaatgcg tcctcgagac ccgcggtgcc tgcccgcgcg atgagctcgt
     4021 cggcggtccc ggccgcgacg acgcgcccct gcgacagcac gacgatgcga tcgcaaagcg
     4081 ccgccacttc ctgcatcacg tgcgacgaga acagcaggca ctttcccgcg ctgcgaagcg
     4141 cgcgcagctc gtcgcggagc gtgcgcacgc tcatgatgtc gaggccgttc gtgggttcgt
     4201 cgagcaggat cgtgtgcggg tcatgcacga gcgcacgcgc aatggcgacc ttcatcttct
     4261 cgccctgcga gaagccggcg gtgcgccgct cggcaatcgc gccgaggccc agcgtggcga
     4321 aaagcgtgtc gatccgcgca tcgaggctcg cgcccgacag gccgtgcagt gcgccgaaat
     4381 agcggacgtt ctcgcgcgcg gtcagccggg gatacaaccc gcgggcgtcg gatagcacgc
     4441 cgatccgcgc gcgcaccgcg tagcggtcgg cgacgacgtc gaggccgtcg acattcgcgt
     4501 gaccttcgtc cgggatcacg agcgtcgcga tcatccgcag cagcgtcgtc ttgcccgcgc
     4561 cgttggggcc gaggagcccg gtgatcgcgc cgtcgggcgc atcgaacgtg acgtcgcgaa
     4621 cggcccggat gtcgcgccgc ttgccgaatg ccttcgcgat gtcgcgaacc gcgatcaagg
     4681 ctgcggcgcc agccgatcgg gccacgaggg cggccgtttc gaatgcgcga aaaagtcgat
     4741 gcagtcctgc gacagcgtgt cgaagcccgc ggcgtcgacg aatgctgcga tgaggcgcgg
     4801 ggcgcacgca tgcggcgaca cgatatggcc gaagcccgcg gcgatcacgt ggcggctgtt
     4861 cggcaatccc ttcgccacct cgtcgccata gacgggcggc gtcacgggat cgaggccgcc
     4921 ggacagcagc agcaccggca gcgcactttg caccggcatc gtgaaatcga ccggatagcc
     4981 gcccttcggc cactggtcgc acacggcgag cgcgcgccgc gcgagcgagc gcacgcgcac
     5041 gtcggcgacg ccgttcgcgc gctcgtcgcg agtcacgcgc ggcgcgtcct cgctgcatgt
     5101 caccgagtag aacagcgccg ggttgaattc atacggaaga tcgccgacga cggtcagcga
     5161 tgccgcaagg agcggcgcgt actggccgtc gtgcgcgagc gcgagcagct ccgggatgag
     5221 gctcgccgct tccggcgaat acgtgagcgg ttgcagtgcg ccgatgatcg tgtcgaactc
     5281 ggcgcggatc tcgcgcggca cgcccgtacg cggatcgcgc acgctcaccg ttcgtccgcc
     5341 ctcgagcgcg cggcggatgt cttcgagcgt tgccgccgga tcgggatgcg ccttggcgca
     5401 cggcttcgac gcgcggcagg cggcgatcac gccgtcgagc gcatcgtcgc gcgtgcgcca
     5461 cacgtcgaac gagatccgca gcgacggcgg cgcgacaccg tcgagcacga cgctgcgcac
     5521 gtgcgaggga tggcggcgca ggtattcctg cgctacgcgc gtgccgtagc tgccgcccca
     5581 caggttcagc ttgtcgtagc cgagtgcctc gcgcatttcg tcgaggtccg cgacccacgc
     5641 gctcgtcgtg tactgcgacg cgtcgacgcc cttgccggca agctgccatg cgcaaagcag
     5701 cgccttgggc accggatcgg tgtcgaattc tgcatgctcg tccggcgcga acgcgggaca
     5761 cgcgagcggc gacgagcgcc cggtgccgcg ctggtcgatc agcacgatgt cgcgcgtgcg
     5821 ccgaatcgcg gtgagcgaaa gcgcgaacgg gccgagcgtc gacgcggcct ggcccggtcc
     5881 gccggcgaga agcaccagcg gatcgggttt gggcgacaac gtattcgcgg gcagcagcgc
     5941 aacgaagatc gacagccggc gtccgttcgg ctgccggcga ttttcgggaa cctccagcgt
     6001 gccgcattgc acggcctgcg caaccttcgg cagacggcat tcggcgagtg tgagccggcg
     6061 cgttgcgggc gcacgatcgc tgcaaccggc gacgagcagc aggcagccga gcaggccgac
     6121 aacgccgcaa aggcgcaatg tccgcgtcat cgggcggagc atcggtcgtg cgcgtcgcga
     6181 agcgtaagat gcatgccggt cgaagtcgac gccgcgaagg cggcgtgcgc tggaaggatc
     6241 atggcggaac tcgagcccgg tattgtcacc gatttgcgcg accgtcaaag ctacggcggt
     6301 tatctgcgtc tcgaccggct gctcgatgcg cagcagccgg tgtcgggcgg cgcggacgag
     6361 acgccgcggc acgacgagat gctgttcatc atccagcatc aggtttccga gctgtggatg
     6421 aagctcatga tccacgaact ccgcgcggcg atcgcgcacg tgcgcgccga tgcactcgag
     6481 ccgtgcttca agattctggc gcgcgtcaag ctcatccaga agcagctctt cgagcaatgg
     6541 gcggtgctgg aaacgcttac cccgtccgaa tacgaggcgt tccggccggc gctcgggacg
     6601 gcgtcggggt tccagtcggc gcagtaccgt gccatcgagt tcctcctcgg caacaagcat
     6661 gcggcgatcc tcgacgtctt ccgccacgag cccgcgacgt tcgccgagct cgacgcgctg
     6721 ctgcacgcgc cgtcgctcta cgacgaattc ctgcgcctgc tggcacggcg agggcttccg
     6781 atgccgaacg catcgcttgc gcgcgacgtc accaagccgt acgtgcgcaa ccccgagctc
     6841 gtcccggtgc tcgctcgcat ctacgcggat cccgagcaat ggtgggatgc gtacgacatg
     6901 tgcgaaaagc tcgtcgacgt cgaggaggca ttccagctct ggcgctttcg ccacatgaag
     6961 acggtcgagc ggatcatcgg ctacaagccg ggaaccggcg gctcgtcggg tgttgcattc
     7021 ctgcgccgcg cgctcgagca ttcgttcttc cccgagctca tcgacgtgcg cacggtgatc
     7081 ggcgcgcgat gagcagcggc ccgatcgagg ccgcggtcgc ggcgctgggc aacgctccgc
     7141 tgaccgaggc ggcgctcgcc gcgcacatcg caccgctttt ttcacgcatg cgcgaagcgg
     7201 cgcccgatcg cgtctacctc gcgaaccatt cactcggccg tccgctcgac gcgatggagc
     7261 acgatgtgcg cgaaggactt gccgcgtggt acgcgcggat ggggggtgcc tgggacgcat
     7321 ggatggacga gatgcaggcg tatcggatgc gccttgcgcg tctcatgcat gcgccgcgca
     7381 gcgactgcgt cgttccgaag acgagtgccg ggcaggggct gcgcgcgatt ctcaatacgt
     7441 acgaccgtgc cgcgcgtccg cgcgtcgtcg caacgcgtgg cgaattcgac tcgctcgacg
     7501 tgatcctgcg cgaatacgcg cgccgcggcc gcatcgagct cgcgctcgtc gagccgcgcg
     7561 ctggcggcag cttcgacgcc gccgacatcg ccgatgcgat cggcaaccgc gtcgatctcg
     7621 tcgtcgtctc cgaggtcatc ttcaacaccg gccagcgcat cggtcgcatc gatgccatcg
     7681 tcgagcgcac gcatgcggcc ggtggccgtg tgcttctgga tgtctaccac tcgctcggcg
     7741 tggttcccgt cgacgtcacg gcgcaggacg tcgactttgc cgtcggcggc agctacaagt
     7801 acctgcgcgg cgggccgggc gcctgcttcc tgtatctcca tccgcgccat ctcgacggcg
     7861 cgctgctgcg tacgctcgac atcggctggt tcgcgaagcg cgagccgttc gcgtatgcgc
     7921 ggccggatcc gcccgagctc gcacccggcg gcgatgcctt tctggaatcg acgccgcccg
     7981 cgctgacctg gtatcaggcg cgcgcgggcc agcggctggc gctggcactc gacgtcgacc
     8041 gcgttcgcgc gtattcgctg cagcagcagc aaacgctcgt cacactgctg cgcgcacgcg
     8101 gcatcggcgc acaaggcggc tccccggacc atggcgcatt cgtcgtcgtc accgatccgc
     8161 gcgcgcaatc gtggtgcgcg acgctcgcga cgcgcggcat cgtcgccgat gcacggggcg
     8221 agaggctgcg gctttgcccg gacgtcctga cgaccgagga acagctcgtg cgtgccgccg
     8281 aggcgctcgg cgcgatcgcc cgtcgctgac ggcaaggcga gcccgggcag ggccgcctcg
     8341 cgctccaaag gctaattgtt gtcgttttcg atcgaaggga tcgagcgcag atactcgtag
     8401 atcgccgtca ggtcgcggtc ggtctttttg ccgaacgacg gccacggcat cacctgcagc
     8461 agcgtgcccg gcggatcgag cggattgtgt cccgtgcgca gcgtcgagat gaattgctcg
     8521 agtgtcaatc cggcgggacg gcccagcgcg tcgggcgtga tgttcgccga cgtgaagggc
     8581 ccgaagtgct gcccgcccgc caggtacccg gcggtattga cttgttcggg ctcgccaaag
     8641 aacggattgc cgccaggagc gaacgacgga ttcgtgtggc actcgctgca gccgccgctg
     8701 ttgacgatgt agctgccgag gccgacgagc gcgcgattct tgcccctgag atcgagcggc
     8761 accggattga tttcgaagcc gcgctgaatc tggctctggc tgaactcgtc ggccgcggcg
     8821 ccggcagcgg gtagaaccat cagcgcaagg cccgccgcgg cagcgcccgc cgcggcgatc
     8881 gatcgcatac gttgcatcgt ttgctccttc acgaattcgg cgttgccaag gagggcaacc
     8941 ggccgaggcg caagtctagg cgggcagcat gccggctgca aggcggattg tcggctcggc
     9001 agcacgcgcc gaccgatcac gcgttgcgcg tgatgatgcg cacgaggatc agcagcgcga
     9061 cgacgagccc ggcggcgatc aggatgccga cgatgatcac ctggtgcggc ttgatcgtga
     9121 cgatatcgcg ccgcatcgcg tcgcccgtgc gcacgccgaa gaagctccag aaaacggcac
     9181 cgacgatctg gcggagtgtc gcctgccgcg gcggaggagg ggtcgtggct ggatcctgcg
     9241 cgggaggagg cgttcgcggc ggttcggtca tcgtggctgt cgtggcaagg cgccgtttcg
     9301 atggtagcac cgtcgcgtcg gcgaaacggg tgcctgtttt ctcgcgataa aggccttccg
     9361 gcgcttgaaa cacgtgccgg attgcctcat ttctttgcca ttcgtccaat tccgtccctc
     9421 tttcgaccat gcgcttcgac aagctcacca ccaagttcca gcaggccctg gccgacgccc
     9481 agagcctcgc cctcggccac gacaacggct tcatcgagcc gcaacacctg ctcgccgcat
     9541 tgctcgctca ggaggatgga ggcaccgcat cgctgctcgc ccgcgccggc gtcaacgtgc
     9601 cgcgcgtgca agccggtctt cgcaccgaga tcaacaacct gccgaaggtc tcgggcaccg
     9661 gcggcgagat cacgatctcg cgcgatctca acaacctgct caatctcacc gacaaggaag
     9721 cgacccggcg cggcgaccag ttcatcgcga gcgagctctt cctgttggca ctcaccgacg
     9781 acaggggacc cacgggacgg ctgctgaagg acaatgggct cacgcgggcg tcgctcgaag
     9841 cggcgatcga cgcggtgcgc ggcggcggca acgtcgactc gcaggaggcc gaaggtcagc
     9901 gcgaggcgct cgccaagtac acgctcgacc tcaccgagcg cgcccgcgaa ggcaagctcg
     9961 acccagtgat cggccgcgac gacgaaatcc gccgcacgat ccagatcctg cagcggcgca
    10021 cgaagaacaa cccggtgctg atcggcgagc ccggcgtcgg caagacggcg attgtcgagg
    10081 gtcttgcgca gcgcatcgtg aacggcgagg tgccggagac gctcaagaac aagcgtgtcc
    10141 tgtcgctcga catggcgcag cttcttgcgg gcgccaagta ccgcggcgag ttcgaggaac
    10201 ggctgaaggc cgtgctgaag gacatcgcgg ccgatgccgg ccgcacgatc gtcttcatcg
    10261 acgaattgca cacgatggtg ggcgccggca aggccgaggg cgcgatcgac gccggcaaca
    10321 tgctgaagcc cgcgctcgcg cgcggcgagt tgcactgcgt gggcgcgacg acgctcgacg
    10381 aatatcgcaa gtacatcgag aaggacgcgg cactcgagcg acgcttccag aaggtgctgg
    10441 tcgacgagcc gtccgtcgag tcgacgatcg cgattctccg cggcctgcag gagcgctacg
    10501 agatccacca cggcgtcgac atcaccgacc cggcgatcgt cgccgcggcc gagctttcgc
    10561 atcgctacat caccgaccgc tttctacccg acaaggcgat cgacctgatc gacgaagcgg
    10621 ccgcgcggat caagatggag atcgactcga agcccgaggc gatggaccgg ctcgaccggc
    10681 ggatgatcca gctcaagatc gagcgcgagg cggtgaagaa ggagaaggac gaagcgtcgc
    10741 agcggcgtct cgccgcgctc gaggacgaga tcgggcgcct ggagcgcgag tacgccgacc
    10801 tggaggaggt gtggaaggcg gagaaggcgc aggtccaggg atcgcagcac gtgaagg
//
LOCUS       DASSVX010000147        26559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_755152, whole genome shotgun sequence.
ACCESSION   DASSVX010000147 DASSVX010000000
VERSION     DASSVX010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 26559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26559
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755152"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFO53_06440"
     CDS             complement(<1..591)
                     /locus_tag="VFO53_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875951.1"
                     /translation="MAAVAGTPSIVAPAVHSAARAHPLRRLLARPAAAAGVIVIGVFV
                     VAAVLAPLIAPYDPIATNFAAVRKAPSAAHWFGTDEIGRDVLSRVIFGARASLEAGVL
                     SVLISLAIGVPAGLLAGYAGGKVDMLISRVTDALLACPFLILAIALAAFLGPSLANAM
                     IAIGISATPVFVRLTRGTTLAAKAEDYVLAARAIGNS"
     gene            complement(598..1542)
                     /locus_tag="VFO53_06445"
     CDS             complement(598..1542)
                     /locus_tag="VFO53_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8875952.1"
                     /translation="MIAYLVRRVATIVPTLVFASILIFALQQLLPGDPAMVLAGEERD
                     PAVIAHLRETLHLDQPFPVRYLYWVRGVVHGDLGESLRMQQPVRTLIAEKLPVTLELA
                     CIAIVIALAIGIPAGVVSAVRRGSAWDYGANVVALWGLSTPNFWLGIMLILLFSVTLG
                     WLPASGYVSPFEDWRANLAAMIMPAFVLGNAIAAVLMRHTRSAMLQVLSSDYVRTARA
                     KGVRERDVILKHALRNALIPIITLGALEFGTLLSGAVLTEQVFTIPGFGKLIVDAVFN
                     RDFAVVQGVVLFTATAYIVLNLVADILYAVANPRMRVG"
     gene            complement(1646..3160)
                     /locus_tag="VFO53_06450"
     CDS             complement(1646..3160)
                     /locus_tag="VFO53_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8875953.1"
                     /translation="MTQPLRRLLLALGCTLALAAGASAQTLRIGLAEDPDVLDPTLAR
                     SFVGRIVFSALCDKLFDIDEKLAIVPQLAASYQWSPDNKALTIKLRDGVVFHDGEKMD
                     AAAVKFSLERHKTMPGSNRRGELAPLSSVDVVDPLTVRLNLSAPFSPLLAQLADRAGM
                     IVSPKAAQAEGDKFGAKPVCAGPFRFVERVAQDRIVLERFPNYWDKANVHFDRIVYLP
                     IPDSTVRLANLRSGQLDFIERLAPSDVAQVKGDARFKLTKITEIGYQGITINVGKSEL
                     AQKNPLGRDPRVREALELALDRDGIVQVVMDGEADPGNQWVADTNTFYAKNVPMPKRD
                     VERAKALLKEAGVANPSFTLMTPTTADAQRIAQVVQAMAREAGFDVKIQSTEFATSLD
                     LADKGQFDAYVLAWSGRADPDGNIFSFDACKQPLNYAGYCKPDVDDLLKRSRTTLDTN
                     ERRKVYAELAAIVLKDRPIVYLYHRHWLWAYASKLDGLRPIPDGLVRVTGLRFK"
     gene            3355..4896
                     /locus_tag="VFO53_06455"
     CDS             3355..4896
                     /locus_tag="VFO53_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875954.1"
                     /translation="MIRCAGKWTIALSMLLCVSARAATLEAIEYYNATLDHYFVTALA
                     DEIAPLDAGAFAGWQRTGQHFTVFDPATPMAGALPVCRFYGIPAAGLDSHFYSASAAE
                     CAAVLQRFPGVWVEETDNAFGIFLPDAATGQCPANSVPVYRAWNGRVDSNHRFTTDPA
                     TLQAMVAKGYVAEGYGPPPMPVAMCSPGGGTAAGVPACIVSASSTSAHIGDTVTLTAS
                     CTNAPVAFTWTNCMSTGAQCSATSATAGSVTYSVIARNGTGASAPASVGVLWQPLPSA
                     PKCTIARTSQTDPPTVNGFAALEVNCDASIGNYSWNGCSSTTRTCIAHESTPGVHTYS
                     VIARNAGGSSAPVTLSLNWVTSAAPPGLCGSFPSYLYSDVGSQSVRVESVLMRAPAFA
                     WNGAWTVHFVVPATMSAASVGSMSSVEFSGPTVVREVTISRTPCDFRATDPSGANGPV
                     ARQSGNSNTIQFTAAPRPGYPLLNAGATYYYSVRNFQPATGTISCPSSPGRCDALVES
                     VLPTR"
     gene            5014..5160
                     /locus_tag="VFO53_06460"
     CDS             5014..5160
                     /locus_tag="VFO53_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875955.1"
                     /translation="MAKPNFEFQKRQKELEKKRKKEEKKQKKLDRAAAQTPGDAEPPP
                     AETS"
     gene            complement(5168..5791)
                     /locus_tag="VFO53_06465"
     CDS             complement(5168..5791)
                     /locus_tag="VFO53_06465"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8875956.1"
                     /translation="MAEPDHRAIASGTLAHYEAQAQAFRDATRDHDVRQNVDALLSRI
                     DAPPPFTILDLGCGPGRDLCTFTSLGHVAVGLDGTASFVTMAREASGCDVWQQDFLQL
                     ALPDSRFDGVFANASLFHVPVAALPRVLAEIRGTLKRGGVLFASNPHGHNEEGWNRGR
                     YGAYLDFTTWSAHVTGAGFTLLDHYYRPSGRPRDQQPWLATVWRRAG"
     gene            complement(5795..8902)
                     /locus_tag="VFO53_06470"
     CDS             complement(5795..8902)
                     /locus_tag="VFO53_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET8875957.1"
                     /translation="MKSPDVQPLVFHPRGDDAAWQRLRRAIRGTLLTDVASRGRYATD
                     ASIYQVEPIAVLVPEDDDDVRAAMEHCAEMRLPILPRGAGSSQCGQTVGAALVIDHSK
                     HLDRVVAFDAEAGTVTVEPGIVLDSLNAWLRRHALWYPVDVSTSAQATLGGMAGNNSC
                     GSRSLAYGNMVHNVVAIDARLSDGTEAWFGPEHAMREASPRVHELLGSLRDIGLRERD
                     EIERVVPKVLRRVGGYNVDVFNPQSVRPYTADGAVNFAQLLVGSEGTLAWTRTLTLQC
                     APLPRHRALGVVNFATLHDAMDSARHLAALGPSAIELVDRTMIDLARGNPAFAPVIER
                     ALIGAPQAILLVEFIADDETAVLRKLDDLDTLLGDIGLAKRLVRMPAADAQKALWDVR
                     KAGLNIMMSLRGDGKPVSFIEDCAVPLEHLAAYVDRLTGIFARHGTRGTWYAHASVGT
                     LHVRPILDMRRDGAEKMRAIAEETAAIVREYKGAFSGEHGDGLVRSEWVQWQFGPRLT
                     QAFERIKDLFDPAGLMNPGKIVRASRMDDPTLFRFAPTYRTVALDTGLDWSAWNVQSD
                     AMSGAITPPGSGGDAAQGFAKAAEMCNNNGHCRKFDAGTMCPSYRVTRDEIHLTRGRA
                     NTLRLALSGQLGDTFASDAVREALDLCVSCKGCRRECPTGVDMARMKIEFQYQWRRSH
                     RMPLGDRLIAHLPRWAPYASSVAPLLNLRDAVPGLARLLERPTGIAAQRSLPRFRYNT
                     FFRDYRDGAAHDLAPVDVVLFVDSFTNAFEPENAHAALRVLRAAGYRAEVATPSANDA
                     QRARPLCCGRTFLAYGLVDEARHEARRVIDALAPCVAAGATIVGLEPSCLLSLRDEFL
                     ALRLGDAAQRIAERALLIEEFLARERAGGKARIALGALPEKRALVHGHCHQKAFDAAG
                     ATVDVLRWIPELAVEPIESSCCGMAGSFGYAARHYDVSMRMGELSLLPAVRSAGADTL
                     VVADGTSCRHQIADGTRETGERQAVHAIRVLARSVQRASGPERRRRRTDRLRDALGVP
                     VRDRG"
     gene            9061..10071
                     /locus_tag="VFO53_06475"
     CDS             9061..10071
                     /locus_tag="VFO53_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HET8875958.1"
                     /translation="MKHFVVDALRATRRAALVIVAAGCAALVAPAALAQTEIKLGHVG
                     EPGSLFQKSADEFAKRANAKLGGKAKVIVYGSSQLGGDKEMIQKLKLGTLDMALPSTV
                     MSSEVDIAGIFEMPYIVKDRAHMGRIEKDVFWPMIEPAIEKKGLTVLAVWENGVRHIT
                     NNKHPIKSPADLTGIKLRVPEGKWRVKMFQAYGANPSPMKFSELFTALQTGVMDGEEN
                     PLTQIYSAKLQEVQKYLSLSGHVYTPAYLTVGTKRWTALPADVRKVLADTAKETQAFV
                     YETAARDEEDLLGKLKQAGMQVNDVDKDAFVAASKPIYDEFAKEVPGAAAAIERANAL
                     RK"
     gene            10068..10580
                     /locus_tag="VFO53_06480"
     CDS             10068..10580
                     /locus_tag="VFO53_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HET8875959.1"
                     /translation="MSGFRNGYGRFLEWVVSALMIILFAEVTLGVVFRAIGASLIWYD
                     EIASVLLAWLTFYGAALASVKRAHIGCPELVDALPWAARRIVNIVAQILVIAFFLLLG
                     GVGIAIMPVLATDALVSVPEIPMSVVQSVIPISSALIVIAELLHLADLVRRRGPVEPA
                     GGLSLSEGLH"
     gene            10620..11903
                     /locus_tag="VFO53_06485"
     CDS             10620..11903
                     /locus_tag="VFO53_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET8875960.1"
                     /translation="MTMLLLFVAVLALVLIGVPIAVSLGIVALVAMVSTHGVDILPNL
                     AVVTYNGATSFPLLAIPLFILAGSIMNASGISRRLIAFASSVFGWIRGGLAHVSIGAS
                     LFFAEISGSAVADVAALGSILIPGMKARGYPAPLAAAVMSSAATLAVIIPPSIPMILY
                     AVMAETSVVQLFVAGIVPGIIGGLGLMAMAYVFARRYDLPREQAFSWANVGKTAREAL
                     WAFLLPIIILGGIFGGVVTATEGAALAVVASLFVGLVIYRELDVAELRRAIIEGGVQT
                     AVVMLLVATSALLGTYLSEVQAPQHLAQAVSDFTQNKYLVLALLNVLFLLLGMFLHSA
                     AAIILVVPVVMPLVRAVGIDPVHFGLIVTVNLGIGQQTPPVASVLMVASAIAKASIWS
                     VTRVNVWFIAVLVAVLLLVTYVPVTGLGLVQLFYR"
     gene            11900..12691
                     /locus_tag="VFO53_06490"
     CDS             11900..12691
                     /locus_tag="VFO53_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET8875961.1"
                     /translation="MTPPSGDEILVTRDGATATVTLNRPQKLNALTRLMWRDLGAAFA
                     ALSGDRDLRCIILRGAGERAFSPGNDIGEFAHERSNKRQARDYGAFMHATARALAACP
                     IPMVAQIHGICVGGGLEIAALCDLRICGASSRFGAPIKNLGLVMAYAEMAPLVRLVGP
                     GVALEILLEGRIFDAAEAKQKGLVTRVVPDADVAAEAAATAARIAEGAPLAARWHKKF
                     ARRLADPAPISQAEADECFDCFDTEDFRTGYAAFLAKQKPAFKGR"
     gene            12691..13953
                     /locus_tag="VFO53_06495"
     CDS             12691..13953
                     /locus_tag="VFO53_06495"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8875962.1"
                     /translation="MENASRPGATAKPAVASPHPGPLRGMRVLELAQIMAGPTCGMLL
                     ADMGADVVKVEKLPGGDDARGYREPRVNGVSAPFMMLNRNKRGIALDLKLPAGRDVLL
                     RMARDADVLTENFRRGTLEKLGLGYDVLSAINPGLIYCAISGYGRNGPAADKGGFDLI
                     AQGFAGLMSITGEPGGPPVKTGNSVADINAGILAATGILAAYVHRLRTGLGQVVDTSL
                     MEAALQQTYWHAAIYFATGESPGPAGSAHLLTAPYQAFHTRDGWINIGGANQANWERI
                     ALVLGHPEWREDPRFATNSARMANLPALVDAMNAMLATRTNAQWIAAFDAAGVPVGPV
                     HTIGEALEHPQTLAREAVVALAHPQAGSTKALGCPLHFSRTPTSITRPAPMLGEHTRE
                     LLHEYGYDDGEIDVLAAQGVVATTASVD"
     gene            complement(14023..14784)
                     /locus_tag="VFO53_06500"
     CDS             complement(14023..14784)
                     /locus_tag="VFO53_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET8875963.1"
                     /translation="MMLAAFTAIPLGFAATASASMLVFDLDVDSCSGGCGPAGTLFGT
                     VTLSTVDADTVHVDVDLSQALVSYFIQTGAGYALTWNGPSGETVSNMSPATGFDFLGY
                     SDPGAKKGGYSTGGNFGIFSYAIECDVAPKDAVDPLAYCASSPGGGGAQYSDLSFDVT
                     LSPALTLANFMATNPQGFYFSVDLLGPSGKTGVIGANSFQECSPTTPGCVAQECLPTT
                     PGCTPSVPEPGTLALIGVSLLSGLAFKRRRTYHIV"
     gene            complement(15102..15884)
                     /locus_tag="VFO53_06505"
     CDS             complement(15102..15884)
                     /locus_tag="VFO53_06505"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8875964.1"
                     /translation="MAPMHNTPAKAPIPDWEARYRRKRAHRGRGLADLEERVGDVCVE
                     IDQRLAGRPRVRVLELGCGYAIALLDLVERYGARVEVCGINLREDAGQAEILRREAKA
                     RGIDVDALPNRRPTLVHGDVAHGLPFADDSFDVVVSQVAWRYFRNKIGVLRDVVRVLR
                     ADGVGLIDADEFDARLPPEYGRLVEIWDERVLVPFADYVAHSGMSFVPVARGRALRLV
                     KAAPFGEELVPRLEIDTQAIDPRWDGIKCVYAKGRSGQAPDL"
     gene            15942..17156
                     /locus_tag="VFO53_06510"
     CDS             15942..17156
                     /locus_tag="VFO53_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8875965.1"
                     /translation="MERLDNARILIYSHDSFGLGHLRRCRAIAHAIVDRYKGVSVLIL
                     SGSPIIGSFDFLPRVDFVRIPGVVKLTNGEYQSLGLHIDLDQTMAMRAAIIQQTAESF
                     RPNLFLVDKEPLGLNGEVVRTLRMLKARGTTLVLGLRDIMDEPALLLREWKRKKVLPA
                     LEHLYDEIWVYGLAGFADPLQGVACPAAVRAKMVYTGYLRRSVPQRKTNEPPIHRDPY
                     VLVTVGGGDDGMAVLGWVLAAYEQDRDLPLRALIVTGPFMAPEQQRYFRERAERLGRI
                     EVLTFDTHLEILMERAVGVVAMAGYNTFCEILSLDKRAILVPRVRPRREQVMRAMRAA
                     AIGLVHTLDPEGPHEPALMTHALRNLGEQPAPSERGALQMLDGLEVITDLVADRVGRR
                     TSRRERAVAQIG"
     gene            17212..18372
                     /locus_tag="VFO53_06515"
     CDS             17212..18372
                     /locus_tag="VFO53_06515"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8875966.1"
                     /translation="MIYVQHLLGIGHLMRARLLAEALAMKGFDVHLVTGGMPIGGRLP
                     RDVHTVQLPPIRVSDASFTPLRDAQFEPIDDAYRERRRALVLDAYERARPSAVLFETF
                     PFGRRSLRFELFPLLERIEATRPRPLVVASIREILQVQAKREREREMLDWAARWFDAI
                     LVHGDPRFARFEDTFSFADDLTPPVHYTGFVLGDAVQTTSDQAVRNEIVVSAGGGGVG
                     IELLATALAAQQHSRFGHLTWRVLAGPNIPEAGMERLRRAGSANAIVERSRVDFAALL
                     RRAFVSISQAGYNTTMDVVTSGARPVIVPFTGNGETEQRARGARLAQFGHAVVVDDRT
                     LTPPALAAAVDVAGTRDNFARWDFASDGAARTAVLVQELLETGSLRSAKTAA"
     gene            18369..19157
                     /locus_tag="VFO53_06520"
     CDS             18369..19157
                     /locus_tag="VFO53_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET8875967.1"
                     /translation="MTRFDALARELDRWEALGRRATLWLRDDDACSDSPALRRLFAIA
                     GEHAVPVTIAAIAATADSTLVDALGVCDEATVVQHGYAHRNHAPPGERSAELGDGRDL
                     RARLDELHRGRARLAQAFGERFAPILVPPWNRAGDALLPHLAAAGFAGISRFGPRATR
                     DAAPGLAQVNTHVDPIAWRRDRAFIGADAALERMTAHLAARRSHACDPDEPTGLLTHH
                     LVFADAAFDFVDDLLRHACLHRAVVWLGAKQAFALQPAAVISCQ"
     gene            complement(19141..19794)
                     /locus_tag="VFO53_06525"
     CDS             complement(19141..19794)
                     /locus_tag="VFO53_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8875968.1"
                     /translation="MTTFDDEFYRDILAGMAVYADSRFLRDVLATVTRHQEPGIGVAL
                     NRNQMASKRWLADTLHASAGGRYGTVMILGGWIGALAAVLLHDRRFAIERVVSVDVDP
                     HCAPVAESLNATHVDAGRFEARTADMRELDYASEAADLLINTSCEHVDGFGRWYARVP
                     DGQRLVLQSNDYVAVAEHINCVPDLAAFKAQAPMRDVHFAGQRAMRRYSRFMLIGRK"
     gene            19888..20385
                     /locus_tag="VFO53_06530"
     CDS             19888..20385
                     /locus_tag="VFO53_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET8875969.1"
                     /translation="MDVSQEAEMLRNVPLFSGLSAPELKLLAFTSELHCFAPGEILMR
                     QGDPADCAYVILEGEAEVLARTARGEFVVAVLGRNSVPGEIGVLTDAPRSATVRASKP
                     VRALRVSPEVFLRLASGRPDRALHVMRQLSAHIANDLRAMASLKEELQKAQAAATPGE
                     PGAAR"
     gene            complement(20321..20974)
                     /locus_tag="VFO53_06535"
     CDS             complement(20321..20974)
                     /locus_tag="VFO53_06535"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET8875970.1"
                     /translation="MLIACIRHASTEWNATGRMQGRRDIALSTAGRDEVMHWRLPADL
                     HPSVDWIASPLARAVDTATRLRGRPPRVEPALIEMDWGAWEGCRLAELRASHGDAFAR
                     NEQRGLDFRPPGGESPRDVAARLTCWLATLDAAGEPIVAVTHNGVLRAMLAIATGWDM
                     MDKPPVKLRPATLHRFALGDRERLALVECNVPLATAFSEPRPALPASRPPAPSATLP"
     gene            complement(20968..22107)
                     /locus_tag="VFO53_06540"
     CDS             complement(20968..22107)
                     /locus_tag="VFO53_06540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8875971.1"
                     /translation="MKVAFYAPLKAPDHPVASGDREIARALMQALSLAGHHVTLASRL
                     RSFDARGDAVRQARLAQIGERVGRRLVAKYAGANRPDIWFTYHLHHKAPDFPGPVVSR
                     ALGIPYVVAEASIAPRRRDGAWAAGYACACAAIRGADTVIFVNPADIGEVRKARGQDA
                     PFAMLAPFIDVSRFASRRPARATSADAPTRLITVAMMREGVKLASYRVLAGALALVRD
                     LPFALTIVGDGTARASVESAFARFADRVTFAGARSKDDVAALLQRHDVFVWPAIGEAI
                     GIAFLEAQACALPVIAGATPGVAAVIAAGRGGLVVPAGDVAAFADALRKLIVDAPLRE
                     RMRTEAFAHVRAHHDLPAAAHRLDAILHEVVARHRRAHAGAALSC"
     gene            complement(22104..23372)
                     /locus_tag="VFO53_06545"
     CDS             complement(22104..23372)
                     /locus_tag="VFO53_06545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8875972.1"
                     /translation="MPLGKVAPAIAGGSIGVVLKGYPRLSETFVAQELRALEQRGLSL
                     ALFSLRRSTDAAAHPLHDEIRAPVTYLPEYLHHGPLRVFRAWRKARRLPGYRSALSAW
                     RKDLWRDRTRNRARRFGQALVLASEMPNDIVHLHAHFLHTPASVARYAAMLRGVGWSV
                     SAHAKDIWTIPEWEKREKLATCTWATTCTACNATHLRELAPPARPVDLVYHGIDTLRF
                     PAPRSVRSTADGRGPRSPVTLLAVGRAVDKKGFDDLLRALATLPGEQHWQLLHIGDGP
                     RRPMLERMARELRLADRIRWLGPQVHAVVLDAYRTADIFVLPSRIAPDGDRDGLPNVL
                     LEAQSQRVACVSTRVSGIPELIVDGVTGLLVPPRAPHALGAALSRLIADPSLRCALGD
                     AGYTRTTTLFSLDGGADRLAQRFASCLAPA"
     gene            23506..26142
                     /locus_tag="VFO53_06550"
     CDS             23506..26142
                     /locus_tag="VFO53_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET8875973.1"
                     /translation="MDRSLFRYILRHTWRDQIFLLLLTSISFPLIYVNLELPKRIVNN
                     AIQGKKIPETFLGLHVTQITYLMALSFVLLLLITINGGLKYWINVYSGVIGERTLRRM
                     RHDLYWQVMRFPLPQFKTMSAGEIIPMIVAETEPVGGFIGDSFALPIFQGGLLVTYVL
                     FIFMQDPWLGIAAIALWPPQMVLIPRLQRRINMLSKERVQTARQLSDRIGESVAGFAE
                     IRGNHTTELERADISERLGKIYGIRFDIYKRKFFVKFLNNFLAQITPFFFYSVGGYLV
                     IRGSLSLGALVAVLAAYKDILSPWKELLNWYATKEDVRIKHEQIVSQFEPPGLVDRKL
                     LEDPPEAIPPLAGEIAASGLMYSENGVTNRIDRLSLHIGAGEHVALVGNGNSGREDLA
                     QLVARLNLPSGGRLEVAGVNFADVHQAIPGRRIAYAAQNAHIFTGTLAHNLYYGLKHR
                     PVHAAAYDEAGEKRAAQRVRDAVASGNSPYDIRADWIDYETAGVADATELTAAAVRML
                     KLVEMEREVIGFGFASTANPGRHPELAQKALAARARVRERVSTEHLEACVEFFDRDAY
                     FDNMSIAENILFGTPRRADFQPGALASNPAIVALLQDAGVLQDLYAAGAKIAALMVEL
                     FADVAPDSNLFEQYSFISPEDLPEFRTLVTKLGRRGLAAVSERDKGRLLALTLRLVKA
                     RHRLGVMDDTMPDRILGARAAFRQRYQDRADICEFFEPERFSAALSIQDNILFGRVAF
                     EQASAQGRISALVRDIGFEEGMCDEVVRIGLEFDVGNAGSRLSYSERQRLAIARALIK
                     NPDILVFNEPTSGLDPGTEVRVLRAVLEWAKGRTVLWSLSRPELAREFDRVLVFADGQ
                     LVEEGRFDELHREGKALAQLVA"
     gene            26299..>26559
                     /locus_tag="VFO53_06555"
     CDS             26299..>26559
                     /locus_tag="VFO53_06555"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET8875974.1"
                     /translation="MSSSPQAVFLSYASQDAEAARHICDALRAMNVEVWFDQSALRGG
                     DAWDASIRRHIRECALFLPIISANTQAREEGYFRREWNLAVNR"
BASE COUNT         4320 a         9004 c         9019 g         4216 t
ORIGIN      
        1 cgaattcccg atcgcgcgtg ccgcgagcac gtaatcctcg gctttcgcgg caagcgtcgt
       61 gcctcgcgtc agtcgcacga acaccggcgt tgccgagatg ccgatcgcga tcatcgcgtt
      121 ggcgaggctc ggcccgagaa aggcggccag cgcgatggcg agaatgagga acgggcaggc
      181 gagcaacgcg tcggtcacgc gcgagatcag catgtcgacc ttgccgcccg cgtagccggc
      241 gagaagaccg gcagggacgc cgatcgcgag cgagatcagc accgacagca cgcccgcctc
      301 gagcgatgcg cgcgcgccga agatcacgcg cgacagcacg tcgcggccga tctcgtcggt
      361 gccgaaccag tgcgcagcgc tcggcgcctt gcgcacggcg gcgaagttgg tggcgatggg
      421 atcgtacggc gcgatcagcg gcgcaagcac ggccgcaacg acgaacacgc cgatcacgat
      481 gacgcccgcc gcggcggcgg ggcgcgcgag cagccttcgc agcggatgcg cgcgcgccgc
      541 cgaatgcact gcgggcgcga cgatcgacgg cgtcccggcg acggcggcca tgcgttgcta
      601 cccgacgcgc atccgcgggt tcgcgacggc gtacaggatg tcggcgacga ggttcagcac
      661 gatgtacgcg gtggcggtga acaatacgac gccctgcacg acggcgaagt cgcggttgaa
      721 cacggcgtcg acgatcagct tgccgaatcc gggaatggtg aagacctgct cggtcagcac
      781 ggcgcccgac agcagcgtgc cgaattcgag cgcgccgagc gtgatgatcg gaatgagcgc
      841 attgcgcagt gcgtgcttca ggatcacgtc gcgctcgcgc acgcccttcg cgcgcgccgt
      901 gcgcacgtaa tcggacgaca gcacctgcag catcgcactg cgcgtatggc gcatcagcac
      961 cgcggcgatg gcattgccga gcacgaacgc cggcatgatc atcgccgcca gattggcgcg
     1021 ccagtcctcg aaagggctca cgtatccgga ggcaggcagc cagccgagcg tcaccgaaaa
     1081 gagcaggatc agcatgatgc cgagccagaa gttgggtgtc gacaatcccc acagcgcaac
     1141 cacgttggcg ccgtaatccc aggcgctgcc ccggcgcacg gcggatacga cgccggccgg
     1201 aatgccgatc gcgagcgcga tgacgattgc gatgcacgcg agctcgaggg tcacgggcag
     1261 cttctcggca atcaacgtgc gcaccggctg ctgcatgcgc agcgattcgc cgagatcgcc
     1321 gtgcacgacg ccgcgcaccc agtagagata gcggaccggg aacggctggt cgagatgaag
     1381 cgtttcgcgc agatgcgcga tcaccgccgg gtcgcgctcc tcgcccgcga gcaccatcgc
     1441 aggatcgcct ggaagcagtt gctgcagcgc gaagatgagg atcgacgcga agacgagcgt
     1501 tggcacgatc gtcgcgaccc ggcggacgag atacgcgatc atcgagagcg gtcagccacg
     1561 cgtttcgcgc tccgggcgcc ggcatcccgc tgccgcagcg agaaatgcgt gcctgatccc
     1621 gggactcatt cgtaccgaag tccagtcact tgaaccgcag ccccgtcacg cgaacgaggc
     1681 cgtcgggaat cgggcgcagg ccgtcgagct tgctcgcgta cgcccacagc cagtggcggt
     1741 gatacaggta gacgatcggc cggtccttga gcacgatcgc ggcaagctcg gcgtagacct
     1801 tgcgccgctc gttggtgtcg agggtcgtgc gcgagcgctt cagcagatcg tcgacgtcgg
     1861 gcttgcagta gcccgcgtag ttgagcggct gcttgcaggc gtcgaagctg aagatgttgc
     1921 cgtcgggatc ggcgcgcccg ctccatgcga gcacgtacgc gtcgaactgc cccttgtccg
     1981 cgagatcgag cgacgtggcg aactcggtcg actggatctt cacgtcgaag cccgcttcgc
     2041 gcgccatcgc ctgcacgacc tgcgcaatgc gctgcgcatc ggcggtggtc ggcgtcatca
     2101 gcgtgaacga cggattcgcc acgcccgctt ccttcagcag cgccttcgcc cgctcgacat
     2161 cgcgcttcgg catcggaacg ttcttcgcat agaacgtgtt cgtgtcggcc acccactggt
     2221 tccccggatc ggcttcgccg tccatcacca cctgcacgat gccgtcgcgg tcgagggcca
     2281 actcgagcgc ttcgcgcacg cgggggtcgc gtccgagcgg attcttctgc gcgagttcgc
     2341 ttttcccgac gttgatcgtg atgccctggt agccgatctc ggtgatcttc gtcagcttga
     2401 agcgcgcgtc gcccttcacc tgggcaacgt cggagggtgc aagccgttcg atgaagtcga
     2461 gctggcccga gcgcaggttc gcgaggcgca ccgtcgaatc gggaatcggc aggtagacga
     2521 tccgatcgaa gtgcacgttc gccttgtccc agtaattcgg aaagcgctcg agcacgatgc
     2581 gatcctgcgc gacgcgctcg acgaagcgga acgggcctgc gcacaccggc ttggcgccga
     2641 acttgtcgcc ttcggcctgc gcggccttcg gcgatacgat catgccggcg cgatcggcga
     2701 gctgcgcgag cagcggcgag aacggcgccg aaaggttgag ccgcacggtc agcggatcca
     2761 cgacgtcgac gctcgaaagc ggcgcgagct cgccgcgccg gttcgagccc ggcatcgtct
     2821 tgtgccgctc gaggctgaac ttcaccgcgg cagcgtccat tttctcgccg tcgtggaata
     2881 cgacgccgtc gcgcagcttg atcgtgagcg ccttgttgtc gggcgaccac tggtagctcg
     2941 cggcaagctg cgggacgata gcgagctttt cgtcgatgtc gaacagcttg tcgcacagtg
     3001 cggagaacac gatgcgcccg acgaagcttc gcgcgagcgt cgggtcgagg acgtcgggat
     3061 cctcggcgag cccgattcgc agcgtctgtg cggacgcacc ggcggcaagc gcgagcgtgc
     3121 atccaagagc gagcagcaag cgacgaagtg gctgtgtcat cgatcctccc tgtgcatccg
     3181 atccgcggta gtcgggatgc gaggcgcgcg cttcgcgcat cggccaaatc agcatagcat
     3241 gcgcaaatcg agcgtcgggc ccggtgcggg acggggaact gacggtgcct ccgatcggtc
     3301 cgttgactac cgaacgaaca accggcgccg cgcgccgcct cgaggttctc cccgatgatt
     3361 cgctgcgcag gaaaatggac gatcgcattg tcgatgctgc tgtgcgtgtc ggcgcgggcg
     3421 gcgacgctcg aggcgatcga gtactacaac gccacgctcg accactactt cgtcacggca
     3481 ctcgccgacg agatcgcgcc gctcgacgcc ggcgcctttg ccggctggca gcgtaccggc
     3541 cagcacttca cggtgttcga tccggcgacg ccgatggccg gcgcgttgcc cgtgtgccgt
     3601 ttctacggca ttcccgcagc gggcctcgat tcgcatttct attcggcatc ggccgccgaa
     3661 tgcgcggcgg tcctgcaacg ctttcccggc gtgtgggtcg aggaaaccga caacgccttc
     3721 ggcatcttcc tgcccgatgc cgccaccggg cagtgccctg ccaacagcgt gccggtgtac
     3781 cgggcatgga acggtcgcgt cgattcgaac catcgcttca ccaccgaccc cgccacgttg
     3841 caggcaatgg tcgccaaggg ctatgtggcc gaaggctacg gtccgccacc gatgccggtc
     3901 gcgatgtgct cgccgggcgg gggcaccgcg gcgggcgtgc ccgcctgcat cgtttcggcg
     3961 tcgagtacgt cggcacacat cggcgacacg gtcacgctga cggcgtcatg cacgaacgcg
     4021 cccgttgcgt tcacctggac gaactgcatg agcaccgggg cgcagtgcag cgcgacgtcg
     4081 gccaccgccg gatcggtcac gtactcggtc atcgcgcgaa acggtaccgg tgcgagcgcg
     4141 ccggcgagcg tcggcgtgct atggcagccg ctgccttccg cgccgaaatg cacgatcgcg
     4201 cgcacgtcgc aaacggatcc gcccaccgtg aacggctttg ccgcgctcga ggtcaactgc
     4261 gacgccagca tcggcaatta cagctggaac ggctgctcga gcaccacgcg cacgtgcatc
     4321 gcgcacgagt cgacccccgg ggtccacacc tactcggtaa tcgcccgcaa cgccggcggc
     4381 agcagcgcgc cggtaacgtt gtcgctcaac tgggtgacaa gcgccgcgcc gccgggactg
     4441 tgcgggtcgt tcccgagcta tctctacagc gacgtgggct cgcaatccgt gcgcgtcgag
     4501 agcgtgctga tgcgggcgcc cgcgttcgct tggaacggcg cttggaccgt gcacttcgtc
     4561 gtgcccgcca cgatgtccgc ggcgagcgtg ggctcgatgt cttcggtcga attctcgggt
     4621 ccaaccgtcg tacgcgaggt aacgatctcc cgcacgccgt gcgactttcg cgcgaccgac
     4681 ccatcgggcg cgaacggtcc ggttgcgcgt cagtcgggca actcgaacac gatccagttc
     4741 accgccgccc cgcgtcccgg atacccgttg ttgaacgccg gtgcgacgta ctactacagc
     4801 gtgcgcaact tccagccggc caccggcacg atctcgtgcc cgtcgtcgcc cggccgctgc
     4861 gacgcgctcg tcgaatcggt gctgcctacg cgctgatgcg ctcgatgcgg gcggacgaaa
     4921 tcgccggacg cgcgcacgag gtggcgctgc ggtcgcattt gcggtaaaac cggagcgtgg
     4981 acaatctcgt ccacatgcac gaggacgcaa aacttggcca agcccaattt cgagttccag
     5041 aaacgccaga aagagcttga gaaaaaacgc aaaaaggaag agaaaaagca gaagaagctc
     5101 gaccgcgccg ctgcgcagac gccaggcgac gccgaaccgc cgccggccga aaccagctag
     5161 cccggattca gccggccctg cgccacaccg tcgcaagcca cggttgctgg tcgcgcggtc
     5221 gaccgctcgg gcggtagtag tgatcgagca gcgtgaagcc cgcgccggtc acgtgcgctg
     5281 accacgtcgt gaaatcgaga taggcaccgt agcggccacg gttccagcct tcctcgttat
     5341 ggccgtgcgg attggacgcg aacaggacgc cgccccgctt cagcgtgccg cgaatctcgg
     5401 ccagcacgcg cggcagcgcc gcgaccggga cgtgaaaaag cgacgcgttg gcaaacacgc
     5461 cgtcgaagcg tgaatcgggc agtgcgagct gcaggaaatc ctgctgccag acatcgcagc
     5521 cgctcgcttc gcgcgccatg gtgacgaagc tcgccgtgcc gtcgagaccg acggcaacgt
     5581 ggccaagcga ggtgaatgtg caaaggtcgc gtccgggtcc gcagccgagg tcgaggatcg
     5641 tgaacggcgg aggagcatcg atgcgcgaca gcagcgcatc gacgttctgc ctgacgtcgt
     5701 ggtcgcgcgt cgcatcgcgg aacgcctgcg cctgcgcttc gtaatgcgcg agcgtgccgc
     5761 tcgcgatcgc acggtgatcg ggctcggcca ctgtctagcc cctgtcgcgc accggcacgc
     5821 caagcgcgtc gcgcaggcga tcggtgcgtc ttcgtcgtcg ttccggtccg cttgcgcgtt
     5881 ggacagatcg cgcgagcacg cgaatcgcgt gcacggcctg gcgctccccc gtttcgcgcg
     5941 ttccgtcggc gatctggtga cgacagctcg tcccgtcggc gaccaccagc gtgtccgcac
     6001 cggcacttcg caccgcgggg aggagcgaaa gctcgcccat gcgcatcgac acgtcgtagt
     6061 gacgggccgc atagccgaac gatcccgcca tcccgcagca gctcgactcg atcggctcga
     6121 ccgcaagctc cggtatccag cgcagcacgt cgacggtggc cccggcggcg tcgaacgcct
     6181 tctggtggca gtgtccgtgc acgagcgcgc gcttttcggg cagggcgcca agcgcaatgc
     6241 gcgccttgcc acccgcgcgt tcccgcgcca ggaattcctc gatcaggagc gcgcgctcgg
     6301 cgatgcgctg cgccgcgtcg ccgaggcgca atgcgaggaa ttcgtcgcgc agcgacagca
     6361 ggcaggaagg ctcgagtccc acgatcgtcg ctcccgccgc cacgcagggt gcaagcgcgt
     6421 cgatgacgcg ccgcgcctcg tgcctggctt catcgaccaa accgtacgcg agaaaggtgc
     6481 gcccgcaaca cagcgggcgc gcacgctggg cgtcgtttgc cgacggcgtt gcgacttcgg
     6541 cgcgataacc ggcggcgcgg agcacgcgca gcgcggcatg tgcattctcg ggctcgaagg
     6601 cattggtgaa gctgtcgacg aacagcacga cgtcgacggg cgcgaggtca tgtgctgcgc
     6661 catcacgata gtcgcggaag aacgtgttgt agcgaaagcg cggcaacgat cgctgcgcag
     6721 cgatgcccgt cgggcgctcg agcaggcgcg caaggcccgg gacggcgtcg cgcaggttga
     6781 gaagcggcgc aacggacgac gcgtagggcg cccagcgcgg cagatgcgcg atcagccgat
     6841 cgccaagcgg catgcggtga ctgcgccgcc actggtactg gaactcgatc ttcatgcgcg
     6901 ccatgtcgac gccggtcgga cattcgcggc ggcagccctt gcagctcacg caaaggtcga
     6961 gcgcttcgcg caccgcgtcg gaagcgaacg tatcgccgag ttgccccgac agcgcgaggc
     7021 gcagcgtgtt ggcgcgcccg cgggtcaggt gaatctcgtc gcgcgtcacg cgatagctcg
     7081 gacacatcgt gcccgcatcg aatttgcggc agtgaccgtt gttgttgcac atctcggccg
     7141 ccttggcaaa gccctgcgcg gcgtcgccgc cgctgcccgg cggcgtgatc gcgccgctca
     7201 tcgcgtcgct ttgcacgttc cacgccgacc agtcgaggcc ggtgtcgagt gcgaccgtcc
     7261 ggtacgtcgg agcaaagcgg aagagcgtcg ggtcgtccat gcgcgacgca cggacgatct
     7321 tgcccggatt catgaggccc gccggatcga acaggtcctt gatgcgctcg aacgcctgcg
     7381 tgaggcgcgg cccgaattgc cactgcaccc attcgctgcg caccagcccg tcgccgtgct
     7441 ctccggaaaa tgcgcccttg tattcgcgca cgatcgcggc ggtttcctcg gcaatggcac
     7501 gcattttttc cgcgccgtcg cggcgcatgt cgaggatcgg ccgcacgtgc agcgtgccga
     7561 ccgacgcgtg cgcataccag gtgccacgcg tgccgtggcg cgcgaaaatc ccggtcaacc
     7621 ggtcgacgta ggccgcaagg tgctcgagcg ggacggcgca atcctcgatg aacgacaccg
     7681 gcttgccgtc gccgcgcagg ctcatcatga tgttgagacc cgccttgcgc acgtcccaca
     7741 gcgctttctg cgcatccgca gccggcatcc tgacgagacg cttcgccaat ccgatgtcgc
     7801 caagcagcgt gtcgagatcg tcgagcttgc gcagcacggc cgtttcgtcg tcagcgatga
     7861 attcgacgag gaggatcgcc tgcggcgcgc cgatcagcgc gcgctcgatc accggggcaa
     7921 aggcgggatt accgcgcgcg aggtcgatca tcgtgcgatc gacgagctcg atggcggacg
     7981 gcccgagtgc ggcgaggtga cgcgccgaat ccattgcgtc gtgcagcgtg gcgaagttga
     8041 cgacgccgag cgcgcggtgg cgcggaagcg gcgcgcactg cagcgtcagc gtgcgcgtcc
     8101 aggcgagcgt cccctcgctg ccgacgagga gctgcgcgaa attcaccgcg ccgtcggcgg
     8161 tgtacggccg cacgctctgc ggattgaaga cgtcgacgtt gtacccgccg acgcgccgca
     8221 gcaccttcgg caccacgcgc tcgatctcgt cgcgctcgcg caacccgata tcgcgcaggc
     8281 ttccgagcag ctcgtgcacc cgcggcgacg cctcgcgcat cgcatgctcc ggaccgaacc
     8341 atgcctcggt gccatccgac aggcgcgcat cgatggcgac gacgttgtgc accatgttgc
     8401 cgtacgcgag cgagcgcgag ccgcacgagt tgttgcctgc catgccgccg agcgtcgcct
     8461 gcgccgacgt gctcacgtcc accgggtacc acagcgcgtg ccggcgcagc cacgcgttga
     8521 gtgaatcgag cacgattccc ggctcgaccg taaccgtgcc cgcttcggcg tcgaacgcga
     8581 cgacgcgatc gagatgcttg ctgtggtcga tcacgagtgc cgcacccacc gtctggccgc
     8641 actgggagct gccggcgccg cgcggcagga tcggcaggcg catttcggcg cagtgctcca
     8701 ttgcagcgcg cacgtcgtcg tcgtcttccg gcacgagcac cgcgattggc tcgacctggt
     8761 agatcgacgc gtcggtggca tagcgaccgc gcgacgcgac gtcggtgagg agcgtaccgc
     8821 ggatggcgcg acgcaatcgt tgccacgccg cgtcgtcgcc gcgcggatga aacacgagtg
     8881 gttgtacgtc gggcgatttc acgccgccgg cctcgctgct gtcgcctggt ggaacgggat
     8941 gcgccaatgg caaacgcacg cgcacgctgc gccgattata gaaggcgcga cgccgggctg
     9001 ccttgtgacc gatcccggac attgtgtagt atccggacga cagccgtcca aggaggaacg
     9061 atgaaacatt ttgttgtcga cgcgcttcgc gcaacccggc gcgccgcgct cgtgatcgtg
     9121 gcggccggtt gtgcggcgct tgttgcaccg gccgcactcg cgcagaccga aatcaagctc
     9181 ggtcacgtcg gcgagcccgg ctcgctcttc cagaagagtg ccgacgaatt tgccaaacgt
     9241 gccaacgcaa agctcggtgg caaggcgaag gtcatcgtct acggctcgag ccagctcggc
     9301 ggcgacaagg agatgatcca gaagctcaag ctcggcacgc tcgacatggc gctgccgtcg
     9361 accgtgatga gctccgaggt cgacatcgcc ggcatcttcg agatgccgta tatcgtcaag
     9421 gaccgcgccc atatgggccg catcgagaag gacgtcttct ggccgatgat cgagcctgca
     9481 atcgagaaga agggcctcac ggtgctcgcc gtgtgggaga acggcgtgcg ccacatcacg
     9541 aacaacaagc atccgatcaa gtcaccggcg gatctcacgg gcatcaagct gcgcgtgccg
     9601 gaaggcaagt ggcgcgtcaa gatgttccag gcgtatggcg cgaatcccag cccgatgaaa
     9661 ttctccgagc tcttcaccgc gctgcagacc ggcgtgatgg atggcgagga gaacccgctc
     9721 acgcagatct attcggcgaa gctgcaggaa gtgcagaaat acctgtcgct gtcggggcac
     9781 gtgtacacgc cggcgtacct caccgtgggc accaagcgct ggacggcgct gccggccgac
     9841 gtgcgcaagg tcctcgccga cacggcgaag gaaacgcagg ccttcgtgta cgaaacggcc
     9901 gcgcgtgacg aggaggatct tctcggcaag ctgaagcagg ccggcatgca ggtgaacgac
     9961 gtcgacaagg acgcgttcgt cgccgcgagc aagccgattt acgatgagtt cgcgaaggag
    10021 gttcccgggg ctgcggccgc gatcgagcgc gcgaacgccc tgcggaagtg agcgggttcc
    10081 gcaacggtta cggccgcttc ctcgagtggg tggtctcggc gctgatgatc attctcttcg
    10141 ccgaggtgac gctcggcgtc gtgttccgcg cgatcggcgc gtcgctgatc tggtacgacg
    10201 aaatcgcctc ggtgctgctc gcctggctca cgttctacgg cgcggcgctc gcgtcggtga
    10261 agcgcgcgca catcggctgt cccgagctcg tcgatgcgct gccgtgggcc gcgcgccgga
    10321 tcgtgaacat cgtcgcgcag attctcgtga tcgcattctt cctgctgctc ggcggcgtcg
    10381 gcatcgcgat catgccggtg ctcgcaaccg acgcactcgt cagcgttccc gaaatcccga
    10441 tgagcgtcgt gcagtcggtg atcccgatct cgtccgcgct gatcgtcatc gccgagctgc
    10501 tgcacctggc cgatctcgtt cgccggcgcg ggccggtcga acccgcgggc gggctgtcgc
    10561 tgtccgaagg cctgcactga ggcggggcgt cccgtagtga cgagcgaaag cggtttgtca
    10621 tgacgatgct gctgctgttc gtggccgtgc tggcgctcgt gctgatcggc gtgccgatcg
    10681 ccgtgtcgct cggcatcgtc gcgctggtcg cgatggtgtc cacgcacggc gtcgacatcc
    10741 tgcccaatct ggccgtcgtg acctacaacg gcgccacgag cttcccgctg ctcgccatcc
    10801 cgctgttcat cctcgccggc tcgatcatga acgcgtcggg tatttcgcgg cggctgatcg
    10861 cgttcgcgtc gtcggtcttc ggctggatcc gcggcgggct cgcgcacgtg tcgatcggcg
    10921 catcgctgtt cttcgccgag atctcgggct cggcggtggc cgacgtcgcc gcccttggat
    10981 cgatcctgat ccccggcatg aaggcgcgcg gctatccggc gccactcgcg gccgccgtga
    11041 tgtcgtcggc tgcgacgctc gccgtgatca tcccgccgtc gatcccgatg atcctctacg
    11101 cggtgatggc ggaaacgtcg gtcgtgcagc tcttcgtcgc cggcatcgtg ccgggcatca
    11161 tcggcgggct cgggctgatg gcgatggcgt acgtcttcgc gcgtcgctac gacctgccgc
    11221 gcgagcaggc gttctcgtgg gcgaacgtcg gcaaaaccgc acgcgaggcg ctgtgggcgt
    11281 tcctgctgcc gatcatcatc ctcggcggca tcttcggcgg cgtcgtcacc gcgaccgaag
    11341 gtgcggcgct ggccgtcgtc gcgtcgctct tcgtcgggct cgtgatctat cgcgagctgg
    11401 acgtcgccga actgcggcgc gcgatcatcg agggcggcgt gcagacggct gtcgtcatgc
    11461 tgctcgtcgc gacgagcgca ctgctcggca cctacctgtc ggaagtgcag gcgccgcagc
    11521 atctggcgca ggcggtgtcg gatttcacgc agaacaagta cctcgtcctg gcgctgctca
    11581 acgtgctgtt cctgctgctc ggcatgttcc tgcattccgc agcagcgatc atcctcgtcg
    11641 tgccggtggt gatgccgctc gtgcgcgcgg tgggcatcga tcccgtgcat ttcggactga
    11701 tcgtcaccgt caacctcggc atcggccagc agacgccgcc ggtcgccagc gtgctgatgg
    11761 tggcgagcgc gatcgccaag gcgtcgatct ggagcgtgac gcgcgtcaac gtgtggttca
    11821 tcgccgtgct cgtcgcggtg ctcctgctcg tcacctacgt gcccgtcacg ggactcgggc
    11881 tcgtccagct cttctaccga tgacaccacc ctcgggcgac gagatcctcg tcacgcgcga
    11941 cggcgcgacc gcgaccgtca ccctcaaccg tccgcagaag ctcaacgcgc tgacgaggct
    12001 gatgtggcgc gatctgggtg cggccttcgc agcgctctcc ggcgatcgcg accttcgctg
    12061 catcatcctg cgtggtgcgg gggagcgcgc attctcgccg ggcaacgaca tcggcgagtt
    12121 cgcccacgag cggtcgaaca agcggcaggc acgcgactat ggcgcgttca tgcacgcgac
    12181 ggcacgcgcg ctcgcggcgt gtccgatccc gatggtcgcg cagatccacg gcatctgcgt
    12241 gggcggcggt ctcgagatcg cggcgctgtg cgacctgcgc atttgcggcg catcgagccg
    12301 cttcggcgcg ccgattaaga atctcggtct cgtgatggcg tatgcggaga tggcgccgct
    12361 cgtgcgtctc gtcgggcccg gcgtggcctt ggagatcctg ctcgaggggc gcatcttcga
    12421 cgcggcggaa gcgaagcaga agggactcgt cacgcgcgtc gtgcccgacg ccgatgtcgc
    12481 ggccgaagcc gccgccaccg ccgcgcgcat cgccgaaggt gcgccgctgg ccgcgcggtg
    12541 gcacaagaag ttcgcccggc ggcttgccga tccggcgccg atttcgcaag cggaagccga
    12601 cgaatgcttc gactgcttcg acaccgagga cttccgcacc ggctacgcgg cgttcctcgc
    12661 gaagcagaag ccggcgttca aggggcgctg atggagaacg cttcgcgccc cggggccacg
    12721 gcgaagccag ccgtcgcatc gccgcatccg ggcccgctgc gcggcatgcg cgtgctcgag
    12781 ctcgcgcaga tcatggcggg acccacgtgc gggatgctgc tcgccgacat gggcgccgac
    12841 gtcgtcaagg tcgagaagct tcccggcggc gacgacgcgc gcggctatcg cgagccgcgc
    12901 gtcaacggcg tgtcggcgcc gttcatgatg ctgaaccgca acaagcgcgg catcgcgctc
    12961 gatctcaagc ttccggcggg acgcgacgtg ctgctgcgaa tggcgcgcga tgccgacgtg
    13021 ctgaccgaga attttcgccg cgggacgctc gagaagctcg ggctcggcta cgacgtgctg
    13081 tcggcgatca atccggggct catctattgc gcgatctccg gctatgggcg caacggcccg
    13141 gccgccgaca agggcggctt cgacctgatc gcgcagggat tcgcgggcct gatgtcgatc
    13201 accggcgagc cgggcggccc gccggtgaag accggcaatt ccgtcgcgga catcaacgcc
    13261 ggcattcttg cggccacggg catcctcgcc gcctacgtgc acaggctgcg caccggcctc
    13321 ggccaggtcg tcgacacatc gctgatggag gccgcgctgc agcagacgta ctggcacgcg
    13381 gcgatctact tcgccaccgg agaatcgccg ggtcctgcgg gttccgcgca tctgctgacc
    13441 gcgccgtatc aggcgttcca tacgcgcgac ggctggatca atatcggcgg cgcgaaccag
    13501 gcgaactggg agcgcatcgc cttggtgctc ggacatcccg agtggcgcga ggatccgcgc
    13561 ttcgccacca attcggcgcg catggcgaac ctgcccgcgc tcgtcgacgc gatgaacgcg
    13621 atgctcgcga cgcgaacgaa cgcgcaatgg atcgcggctt tcgatgccgc cggcgtgccg
    13681 gtgggacccg tgcacacgat cggcgaagcg ctcgagcatc cgcaaacgct cgcgcgcgag
    13741 gcggtcgtcg cacttgcgca cccgcaagcc ggcagcacga aggcgctcgg ctgcccgctg
    13801 catttttcgc gcacgccgac gtcgatcacg cgccctgcac cgatgctcgg cgagcacacg
    13861 cgcgagctgc tgcacgaata cggctacgac gacggcgaaa tcgatgttct cgcagcgcag
    13921 ggcgtcgtcg ccacgaccgc aagcgtggat tgaagcaggt tcggggaagc gaaaacgccg
    13981 ccggaaaggc ggcgggtttc gcccgggtgg tacccggctg aatcagacga tgtggtacgt
    14041 gcgcctgcgc ttgaacgcca agccggacag gagcgagacg ccaatcaagg cgagcgttcc
    14101 tggctcgggc accgacggag tgcatccggg cgtggtcggc aggcattcct gcgccacgca
    14161 acccggcgtc gtgggactgc actcctggaa ggagttggcg ccgatgacgc cggtcttgcc
    14221 gctcggtccg agaagatcga cggagaagta gaacccctgc ggattggtgg ccatgaaatt
    14281 ggcgagcgtc aaggcaggcg aaagcgtcac gtcgaacgac agatcgctgt actgcgcgcc
    14341 gccaccaccc gggctcgaag cgcagtaagc caatgggtcg acagcgtcct tcggcgcgac
    14401 gtcgcattcg atcgcgtaac tgaaaatgcc gaagttgccg ccggtcgagt acccgccctt
    14461 cttggcgccc gggtccgagt aaccgaggaa gtcgaacccg gtcgcgggtg acatgttcga
    14521 gacggtctcc ccgctgggcc cgttccatgt aagcgcatag ccggcaccgg tctggatgaa
    14581 atacgagacg agcgcctggc tcaagtcgac gtcgacatga accgtatcgg cgtcgaccgt
    14641 cgacagcgtt acggttccga agagcgtacc agcgggcccg caaccgccgc tgcacgagtc
    14701 gacgtcgaga tcgaagacca gcatggaagc gctggccgtc gcggcgaagc cgagcggaat
    14761 cgcggtgaaa gccgccagca tcatcgttgt tgcaatccgg gcgcagggct tgttcataaa
    14821 ctgtttttcg gggaaggtgg tggtggcgtc cccacgaagc acacatcatg cccgactaat
    14881 aatgatgttt attcaatagg ttgcgctgtc acatacaacg cattgtaaaa atcggcgatg
    14941 atcaggaaaa cattgtgacg cacatgctgt aagctgctgt taaacttgct gaattgtgtt
    15001 gcagcttcac ggaaatcggc ttcgtgccgc gctgacgggc acaacgcgcg cttcccgcac
    15061 gctacgccgc gcgagacagg ttgccgctgc gcaaaaccag gttaaaggtc cggggcctgc
    15121 ccggatctcc ctttcgcgta gacgcatttg atgccatccc agcggggatc gattgcctgc
    15181 gtgtcgatct ccagccgagg aacgagttcc tcgccaaacg gtgccgcctt cacgaggcgc
    15241 agcgcgcgtc cgcgcgcaac gggcacgaac gacatgccgg aatgcgcgac gtaatcggcg
    15301 aacggcacga gcacgcgctc gtcccagatc tcgacgagcc gtccgtattc gggcggcagc
    15361 ctggcatcga actcgtcggc gtcgatcaat ccgacgccgt ccgcccgcaa cactcggacg
    15421 acgtcgcgca gaacgccgat cttgttgcgg aagtaccgcc acgccacctg gctcacgacg
    15481 acgtcgaatg aatcgtcggc gaagggcagg ccgtgcgcga catccccatg cacgagggtc
    15541 gggcgccgat tggggagggc gtcgacgtcg attccacgcg ccttcgcctc gcggcgcagg
    15601 atctcggctt gtccagcgtc ctcgcgcagg ttgatcccgc acacctcgac ccgcgcgcca
    15661 taacgttcga cgaggtcgag cagcgcgatg gcatacccgc atcccagttc gagcacgcgc
    15721 acccggggcc ggcccgcgag ccgctggtcg atctcgacac atacgtcgcc gacgcgctcc
    15781 tcgaggtcgg ccaggccgcg gccgcgatgc gcgcgcttgc gccggtaacg cgcctcccaa
    15841 tcggggatgg gtgccttcgc gggcgtgtta tgcatcggtg ccattgtccg ctatcctagc
    15901 cggccgcggc gcaacacggc tcgccgacgg gggacatcgg catggaacga ctcgacaacg
    15961 cgcgcatcct gatctacagc cacgacagct tcggcctcgg gcatctgcgc cgctgccgcg
    16021 cgatcgcgca cgcgatcgtc gatcgctaca agggcgtgtc ggtcctgatc ctttcgggct
    16081 cgccgatcat cggcagcttc gacttcctgc cgcgcgtcga tttcgtgcgc atcccggggg
    16141 tggtcaagct gaccaacggc gaataccagt cgctcgggct gcacatcgac ctcgaccaga
    16201 cgatggcgat gcgcgccgcg atcatccagc agaccgccga gagcttccgg ccgaacctgt
    16261 tcctcgtcga caaggaaccg ctcggcctca acggcgaggt ggtgcgcacg ctgcgcatgc
    16321 tgaaggcgcg cgggacgacg ctcgtgctgg gcctgcgcga catcatggac gagccggcgc
    16381 tcctgctgcg cgaatggaag cgcaagaagg tgctgcccgc gctcgagcat ctctacgacg
    16441 agatctgggt ctacggcctc gcaggcttcg ccgacccgct gcagggtgtc gcgtgtcccg
    16501 ccgccgtgcg cgcgaaaatg gtctacaccg gctacctccg ccgctcggtt ccgcagcgca
    16561 agacgaacga gccgccgatc caccgcgatc cctacgtgct cgtcaccgtc ggcggcggtg
    16621 acgacggcat ggccgtgctc ggctgggtgc tcgcggccta cgagcaggat cgggacctgc
    16681 cgctgcgcgc gctgatcgtc accggcccgt tcatggcgcc cgagcagcag cggtattttc
    16741 gcgagcgcgc ggagcgcctc ggacgcatcg aggtgcttac gttcgacacc cacctcgaga
    16801 tactgatgga acgcgccgtc ggcgtcgtcg cgatggccgg ctacaacacg ttctgcgaga
    16861 tcctgtcgct cgacaagcgc gcgatcctcg tgccgcgcgt tcgtccgcgg cgcgagcagg
    16921 tgatgcgcgc gatgcgcgcc gcagcgatcg ggctcgtgca tacgctcgat cccgagggac
    16981 cgcacgagcc ggccctgatg acgcacgctc ttcgcaatct cggcgagcag ccggcaccgt
    17041 ccgagcgcgg cgcattgcag atgctcgatg gcctcgaggt gatcaccgat ctcgtcgccg
    17101 atcgcgtcgg ccggcgcact tcgcggcgcg agcgggcggt cgcgcagatt ggctgattcg
    17161 tccagcacgc cgcggctgac gcgtagcgca gcgccgcaga tgcgttcggt catgatctac
    17221 gtccagcatc tgctgggcat cgggcaccta atgcgcgcac gcctgctcgc cgaggcgctg
    17281 gcgatgaagg gcttcgacgt tcatctcgtc accggcggca tgccgatcgg cgggcggctc
    17341 ccccgggacg tgcatacggt ccagctgccg ccgatccgcg tctccgatgc gagcttcacg
    17401 ccgctgcgcg atgcgcaatt cgagccgatc gacgacgcct atcgcgaacg ccggcgcgcg
    17461 cttgtgctcg acgcgtacga gcgtgcgcga ccctcggcgg tgctgttcga gacgtttccg
    17521 ttcggccgcc ggtcgctgcg cttcgagctc tttcccttgc tcgagcgcat cgaggccacg
    17581 cgcccgcggc cgctcgtcgt cgcatcgatc cgcgagatcc tgcaagtgca ggcgaaacgc
    17641 gagcgcgagc gcgaaatgct cgattgggcg gcgcgctggt tcgacgcgat cctcgtgcac
    17701 ggcgatccgc gcttcgcgcg gttcgaggac accttttcgt tcgccgacga cctgaccccg
    17761 cccgtgcact acacgggatt cgttctcggc gacgcggtgc aaacgaccag cgaccaggcg
    17821 gtgcgcaacg agatcgtcgt ttcggccggt ggcggcggcg tgggcatcga gctgcttgca
    17881 acggcgctcg ccgcgcagca gcactcgcgc ttcggccatc tcacgtggcg cgtgctcgcg
    17941 ggccccaaca ttcccgaggc gggcatggag cggttgcgtc gtgcaggcag cgccaacgcc
    18001 attgtcgagc gctcgcgcgt cgacttcgcc gccctgctgc gacgggcatt cgtgtcgatc
    18061 tcgcaggcgg gatacaacac gacgatggac gtcgtgacga gcggcgcgcg gccggtgatc
    18121 gttccgttca ccggcaacgg cgagaccgag cagcgcgcgc ggggggcgcg gcttgcgcaa
    18181 ttcgggcatg cggtcgtcgt cgacgaccgc acgctcacgc cgccggcgct tgcggccgcc
    18241 gtcgacgtcg cgggcacgcg cgacaacttc gcccgctggg acttcgcgag cgacggggcc
    18301 gcacgaaccg cggtgctcgt gcaggaactg ctcgaaacgg gatcgctgcg ctcggcgaag
    18361 acggccgcat gacgcgcttc gacgcgctcg ctcgcgagct cgaccgctgg gaggcgctcg
    18421 gccgccgcgc aacgctgtgg ctccgcgacg acgacgcgtg cagcgattcg ccggcgctgc
    18481 ggcgtctgtt cgcgatcgca ggcgagcacg cggtgccggt cacgatcgct gcgattgcgg
    18541 caaccgccga ttcgacgctg gtcgacgcgc tcggcgtttg cgacgaggcg accgtcgtgc
    18601 agcacggcta cgcgcaccgc aaccatgcgc cgccggggga gcgcagcgcc gagcttggcg
    18661 acggacgcga cctgcgcgcc cggctggacg agcttcaccg cggccgcgca cgactcgcgc
    18721 aggcattcgg cgagcgtttt gcgccgatcc tcgtgccgcc gtggaaccgc gcgggcgatg
    18781 cgctgctgcc gcacctggcg gcggccggct tcgccgggat ctcgcgcttc ggaccgcgcg
    18841 cgacacgcga tgcagcgccg ggccttgcgc aggtcaacac gcacgtcgac ccgattgcct
    18901 ggcggcgcga tcgcgcgttc atcggcgccg acgcggcgct cgagcggatg accgcgcatc
    18961 ttgccgcacg gcgctcgcat gcgtgcgacc cggacgagcc gacggggctc ctcacgcatc
    19021 acctcgtgtt tgccgacgcc gcgttcgact tcgtcgacga cctgctgcgc catgcgtgcc
    19081 tgcatcgcgc cgtcgtgtgg ctcggggcga agcaagcatt cgcgcttcaa cccgccgccg
    19141 tcatttcctg ccaatgagca tgaagcgcga gtagcggcgc atcgcacgct gcccggcgaa
    19201 gtgcacgtcg cgcatgggcg cctgtgcctt gaacgctgcc aggtcgggca cgcagttgat
    19261 gtgctctgca acagcgacgt agtcgttcga ctgcagcacg agccgctgcc cgtcgggcac
    19321 gcgggcgtac cagcggccga atccgtcgac gtgctcgcag ctcgtgttga tcaggagatc
    19381 ggccgcttcg ctcgcgtagt cgagctcgcg catgtcggcc gtccgcgcct cgaagcgtcc
    19441 cgcgtcgacg tgcgttgcat tgagcgattc ggcgaccggc gcgcaatgcg gatcgacatc
    19501 gacgctgacg acgcgctcga tcgcgaaacg ccgatcgtgc agcagcaccg cggcgagcgc
    19561 gccgatccac ccgcccagga tcatcacggt gccgtagcgg ccgccggcgc tcgcatgcag
    19621 cgtgtcggcg agccagcgct tgctcgccat ctggttcctg ttgagcgcca cgccgatccc
    19681 gggctcctgg tggcgggtga cggtggcgag cacatcgcgc aggaagcggc tgtcggcgta
    19741 gaccgccatc cccgcgagga tgtcgcgata gaactcgtcg tcgaacgtcg tcatcgggac
    19801 ttccccttgc cggcggcgat gccgtattct gtgggcgcgg ccatgcgccg gccgcgcgga
    19861 agccgcccag gacgacggag acccgacgtg gatgtgtcgc aggaagccga gatgctgcgc
    19921 aacgtgccgc tgttcagcgg cctctccgcg ccggagctca agctcctcgc gttcacgagc
    19981 gagctgcatt gcttcgcgcc gggcgagatt ctgatgcgcc agggcgatcc cgccgattgc
    20041 gcatacgtca tcctcgaagg cgaggccgaa gtgctcgcgc gaacggctcg cggggagttc
    20101 gtcgtggccg tgctcggccg caattcggtg cccggcgaaa tcggcgtgct gaccgacgcg
    20161 ccgcgcagcg caaccgtgcg tgcgagcaag cccgtgcgcg cgctgcgcgt ctcgccggag
    20221 gttttcctcc ggctcgcgtc gggccggccc gatcgcgcgt tgcacgtcat gcgccagctt
    20281 tcggcgcata tcgcgaacga cctgcgcgcg atggcctcgc tcaaggaaga gttgcagaag
    20341 gcgcaggcgg ccgcgacgcc gggagagccg ggcgcggctc gctgaatgca gttgcaagcg
    20401 ggacgttgca ttcgacgagg gccagcctct cgcggtcccc gagcgcgaaa cggtgcagcg
    20461 tcgccgggcg gagcttgacg ggcggcttgt ccatcatgtc ccagccggtc gcgatcgcga
    20521 gcatcgcgcg cagcacccca ttgtgcgtga cggcgacgat cggctcgcct gccgcgtcga
    20581 gcgtcgcaag ccagcacgtg aggcgcgcgg ccacgtcgcg tggactttcg ccgcccggcg
    20641 ggcggaaatc gaggccgcgc tgctcgttgc gggcgaacgc gtcgccgtgg ctggcgcgaa
    20701 gctccgcgag gcggcagcct tcccacgcgc cccagtccat ctcgatcagc gccggctcga
    20761 cacgcggtgg ccggccgcgc agcctcgtcg cggtgtcgac ggcgcgcgca agcgggctcg
    20821 cgatccagtc gaccgatggg tgcaggtcgg caggcaggcg ccagtgcatg acttcatcgc
    20881 gacccgccgt gctcagtgca atgtcgcgcc gaccctgcat ccgtcccgtc gcgttccatt
    20941 cggtgctcgc gtggcggatg cacgcgatca gcatgacaac gccgcgccgg catgcgcgcg
    21001 tcgatgccgc gcgacgacct cgtgcaggat cgcgtcgagc cggtgcgcgg ccgccggcag
    21061 atcgtggtgc gcgcgcacgt gcgcgaacgc ttccgtgcgc atgcgctcgc gcaacggcgc
    21121 gtcgacgatc agtttgcgta gcgcgtctgc gaatgcggcg acgtcgccgg ccgggacgac
    21181 caatccaccg cggccggccg caatgaccgc ggccacgccc ggcgtcgcgc ccgcaatcac
    21241 cggcagcgca caggcctgcg cctcgaggaa cgcgatgccg atcgcctcgc cgatcgccgg
    21301 ccagacgaag acatcgtgac gctggagcag cgccgccacg tcgtccttcg agcgcgcgcc
    21361 tgcgaacgtc acccgatcgg cgaacctcgc gaatgccgat tcgacgctcg ctcgcgcggt
    21421 tccgtcgccg acgatcgtga gcgcgaaggg caggtcgcgc accagggcga gagcgccggc
    21481 gagaacgcgg tacgacgcga gcttcacgcc ttcgcgcatc atcgccacgg taatgaggcg
    21541 cgtcggcgca tcggcgcttg ttgcgcgtgc ggggcgtcga gatgcaaagc gcgaaacgtc
    21601 gatgaacggt gcgagcatcg cgaacggcgc atcctgcccg cgcgccttgc gcacttcgcc
    21661 gatgtcggcg ggattcacga agatcaccgt gtctgcgccg cgaatcgccg cgcaggcgca
    21721 tgcgtagccg gcggcccatg ccccatcgcg ccggcgcggc gcgatcgacg cttccgccac
    21781 gacataggga atgccgagcg cgcggctcac gacggggccc ggaaaatcgg gcgccttgtg
    21841 atgaagatga taggtaaacc agatgtcggg acgattcgcg cccgcgtatt tcgccaccag
    21901 ccgccggccg acgcgctcac cgatctgcgc aagtcgcgcc tggcgcacgg catccccccg
    21961 cgcatcgaag ctccggagcc gcgaggcgag cgtgacgtgg tgtccggcca gcgacagcgc
    22021 ctgcatcagc gcacgggcga tctcgcggtc gcccgacgcc accgggtgat cgggggcctt
    22081 gagcggcgcg tagaacgcaa ctttcacgca ggcgccagac aggacgcgaa gcgctgcgcg
    22141 agccgatcgg cgccgccgtc gagcgagaac agcgtcgtcg tgcgcgtata gccggcgtcg
    22201 cccagcgcgc atcgcaacga cggatcggcg atgaggcgcg acagcgccgc gccaagcgcg
    22261 tgcggcgcac gcggcggcac gaggagcccg gtcacgccgt cgacgatgag ctcgggaatt
    22321 ccggacacgc gggtcgacac gcacgccacg cgctggcttt gtgcctcgag cagcacgttc
    22381 ggcagtccgt cgcggtcacc atccggcgcg atgcgcgacg gcagcacgaa gatgtcggcc
    22441 gttcggtacg cgtcgagcac gacggcatgc acctgcgggc cgagccagcg aatgcgatcg
    22501 gcgagacgca gctcgcgcgc catgcgctcg agcatcgggc gtcgcggccc gtcgccgatg
    22561 tgcagcaact gccagtgctg ttctcccggg agcgttgcaa gcgcccgcaa caggtcgtcg
    22621 aagcccttct tgtcgacggc gcggccgacg gcaaggaggg tgaccggcga tcgcggaccg
    22681 cggccgtccg ccgtcgagcg cacgctgcgc ggtgcgggaa agcgcagggt gtcgatgccg
    22741 tggtagacga ggtcgaccgg ccgcgccggc ggcgcaagct cgcgcaaatg cgtcgcgttg
    22801 cacgcggtgc acgtcgttgc ccacgtgcac gtcgcgagct tttcgcgctt ctcccattcg
    22861 ggaatcgtcc agatgtcctt cgcgtgcgcc gaaacgctcc agccgacccc gcgcagcatg
    22921 gcagcgtacc gtgcgaccga cgcgggcgtg tgcaggaagt gcgcgtgcag atgcacgatg
    22981 tcgttcggca tttcgctcgc gagcaccagt gcctgcccga agcggcgcgc gcggttgcgg
    23041 gtgcgatcgc gccacaggtc cttgcgccag gcggaaaggg cgctgcgata gccgggcagc
    23101 cggcgcgcct tgcgccacgc gcgaaacacg cgcagcggcc catggtgcag atattcgggc
    23161 aggtacgtca ccggcgcccg gatctcgtcg tgcagcggat gcgccgccgc gtcggtcgag
    23221 cggcgcagcg aaaagagggc aagcgaaagc ccgcgctgct cgagcgcgcg caactcctgc
    23281 gcgacgaacg tctccgaaag ccggggatag cccttgagca cgacgccaat cgatccaccg
    23341 gcgatcgcgg gggccacctt gccaagcggc atagggggta ggctttgcac gcagtggcgc
    23401 gatgaggatt cccggagccg cagatgctaa catcatcggc aagcgacgcg ctgcggcgac
    23461 aagggccacc cgccagccgc gtggcgcgtt cgcttccgcg aaagcatgga ccgctccctc
    23521 tttcgttaca tcctccgcca cacctggcgc gatcagatat tcctgctcct gctgacgtcg
    23581 atcagctttc cgctgatcta cgtgaatctc gagctgccga agcggatcgt caacaacgcc
    23641 attcagggca agaagatccc cgagacgttc ctcggccttc acgtgaccca gatcacgtac
    23701 ctgatggcgc tgtccttcgt cctgctgctg ctgatcacga tcaacggcgg gctcaagtac
    23761 tggatcaacg tttactcggg cgtgatcggc gagcgcacgc tgcggcgcat gcgccacgat
    23821 ctctactggc aggtgatgcg cttcccgctg ccgcagttca agacgatgtc ggcgggcgag
    23881 atcatcccga tgatcgtggc cgagaccgaa cccgtgggcg gcttcatcgg cgactcgttc
    23941 gcgctgccga tcttccaggg cgggcttctc gtcacctacg tcctgttcat cttcatgcag
    24001 gacccgtggc tcggcatcgc cgcgatcgcg ctgtggccgc cgcagatggt gctgatcccg
    24061 cgcctgcagc gcaggatcaa catgctgtcg aaggagcgcg tgcagaccgc acgccagctc
    24121 tccgaccgga tcggcgaatc ggtcgctggc ttcgccgaaa tacgcggcaa ccatacgacc
    24181 gagctcgagc gcgccgatat cagcgagcgc ctcggcaaga tctacggcat ccgcttcgac
    24241 atctacaagc gcaagttctt cgtcaagttc ctgaacaact tcctcgcgca gatcacgccg
    24301 ttcttcttct attcggtcgg tggctacctc gtgatccgcg gcagcctctc gctcggcgcg
    24361 ctcgtcgccg tgctggccgc gtacaaggac atcctctctc cgtggaagga gctgctcaac
    24421 tggtatgcga cgaaggagga cgtgcgcatc aagcacgagc agatcgtctc gcagttcgag
    24481 ccgccggggc tggtcgatcg caagctgctc gaggatccgc ccgaagcgat tccgccgctg
    24541 gccggcgaaa tcgcggcgtc ggggctcatg tacagcgaga acggcgtcac caaccgcatc
    24601 gaccggctgt cgctgcacat cggcgccggc gagcacgtgg cactcgtcgg caacggcaac
    24661 agcggccgcg aggaccttgc gcaactcgtc gcgcggctca acctgccgag cggcggacgg
    24721 ctcgaggtcg ccggcgtcaa cttcgccgac gttcaccagg cgattcccgg acgccgcatc
    24781 gcgtatgcgg cgcagaacgc gcacatcttc acggggacgc ttgcacacaa cctgtattac
    24841 ggcttgaagc atcgcccggt gcacgcggcc gcctacgacg aggccggcga gaagcgcgcg
    24901 gcgcagcgcg tgcgcgacgc ggtggcctcc ggcaacagcc cgtatgacat ccgcgccgac
    24961 tggatcgact acgagacggc gggcgtcgcc gacgcgacgg agctgaccgc ggccgcggtg
    25021 cgcatgctga agctcgtcga gatggagcgc gaggtgatcg gcttcggctt tgccagcacg
    25081 gccaatcccg ggcgccatcc cgagctcgcg cagaaggcgc tggccgcccg ggcgcgcgtg
    25141 cgtgagcgcg tttcaaccga gcatctcgaa gcgtgcgtcg aattcttcga ccgcgacgcg
    25201 tacttcgaca acatgagcat tgccgagaac attctgttcg gcacgccacg gcgcgccgat
    25261 ttccagccgg gggcgctcgc gtcgaatccc gcgatcgtgg cgctgctgca ggacgcaggc
    25321 gtgctgcagg acctgtatgc agccggcgcg aagatcgcgg cactgatggt cgagctgttc
    25381 gccgatgtcg ccccggacag caacctgttc gagcaataca gcttcatcag tcccgaggat
    25441 ctgcccgagt tccgcacgct cgtcacgaag ttgggacgcc gcggcctcgc cgccgtttcc
    25501 gagcgcgaca agggacggct cctcgcgctg acactgcgcc tggtgaaagc gcggcatcgt
    25561 ctcggcgtga tggacgacac gatgcccgat cgcatcctgg gggcgcgcgc cgccttccgg
    25621 cagcgctacc aggaccgcgc cgacatctgc gaattcttcg agcccgaacg cttcagcgcg
    25681 gcgctgtcga tccaggacaa catcctgttc ggccgcgtgg cgttcgagca ggcgagcgcg
    25741 cagggacgca tcagcgcgct cgtgcgcgac atcgggttcg aggaagggat gtgcgacgaa
    25801 gtggtgcgca tcggcctcga gttcgacgtg ggcaacgcgg gatcgcgtct atcgtattcc
    25861 gagcggcaac gtctcgcgat cgcgcgcgcg ctcatcaaga atccggacat cctcgtgttc
    25921 aacgagccaa cgtcgggact cgatccgggc accgaggtcc gcgtgctgcg cgcggtgctc
    25981 gaatgggcga agggccgcac ggtgctgtgg tcgctgtcgc ggcccgagct cgcgcgcgag
    26041 ttcgatcgcg tgctcgtgtt cgccgacggg cagctcgtcg aggagggccg gttcgacgag
    26101 ctgcatcgcg aagggaaggc gctggcgcag ctggtggcgt gaggaggagg tgtgggccgc
    26161 acgagcgcgc ggccgcttgc gaagagtcct ttgtaaacgc gattcaattg agtgacaatc
    26221 agcgccggcc ctgctcgggg gccacggcaa ccctggcccc cctgggtcgc agcatgcgtc
    26281 ggtcccgact atccgtacgt gagcagttcc ccgcaagccg tctttctgag ctacgcctcg
    26341 caggacgcgg aggccgcgcg gcacatctgc gacgcgctgc gggcaatgaa cgtcgaggtg
    26401 tggttcgacc agagcgcact gcgcgggggt gacgcgtggg atgcgtcgat ccgccgacac
    26461 atcagggagt gcgcgctgtt cttgccgatc atttcggcca acacccaggc tcgcgaagaa
    26521 ggttacttcc ggcgcgaatg gaacctggcg gtgaaccgc
//
LOCUS       DASSVX010000148         7052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_605638, whole genome shotgun sequence.
ACCESSION   DASSVX010000148 DASSVX010000000
VERSION     DASSVX010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605638"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFO53_06560"
     CDS             complement(<1..208)
                     /locus_tag="VFO53_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875975.1"
                     /translation="MRAIPPLGASPAAPSNARPRRRLLIVGAVAAAALALGAGSAYFV
                     HDANDASRDGSAVLGLVLPDLQGQQ"
     gene            complement(205..672)
                     /locus_tag="VFO53_06565"
     CDS             complement(205..672)
                     /locus_tag="VFO53_06565"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD N-terminal
                     domain-containing protein"
                     /protein_id="HET8875976.1"
                     /translation="MPLLAIAFATAHAEGIASLAPKLLPPERAFHLSARALDASTIEA
                     RFDVADGYYLYRDKMQFTTEPVPAVKPDLPPGKPKHDAFFGDVETYRGEVVVRVPLAQ
                     AAAGKTITLHANSQGCADVGVCYPPNPQQLTLAIPLGGKPGAFVEAGSKGWFK"
     gene            complement(692..1120)
                     /gene="cutA"
                     /locus_tag="VFO53_06570"
     CDS             complement(692..1120)
                     /gene="cutA"
                     /locus_tag="VFO53_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HET8875977.1"
                     /translation="MGTPVQRANLAVTTRSTSGNISTMASIRKRITTPSRDAAILVVT
                     HAPDHAVATELANALLNDRLAACVNIGAAVDSIYHWRGGIETATEVPLAIKTRSALYY
                     NVEDVIRKTHPYDTPEIIAIPIVGGEARYLDWIAAETAPS"
     gene            1041..1436
                     /locus_tag="VFO53_06575"
     CDS             1041..1436
                     /locus_tag="VFO53_06575"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HET8875978.1"
                     /translation="MLAIVLIFPLVDLVVTARFARWTGVPMWMWLAGSAVAGLWVLAN
                     ERVQFRSRALAAFRRDALLRGLLDSGRRVLAGVLLIAPGLLSDVLALLLLLLPINQRG
                     AFGGPQPVAAGRAPYRSDRTLDGDYRRID"
     gene            complement(1476..2294)
                     /gene="lgt"
                     /locus_tag="VFO53_06580"
     CDS             complement(1476..2294)
                     /gene="lgt"
                     /locus_tag="VFO53_06580"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET8875979.1"
                     /translation="MLVHPDFDPVAFHVGPLAVRWYGLMYLAGFILFIVLGRRRARAE
                     MLTGWRMSDVDDMLFFGVFGVILGGRLGYVLFYKPLYYFAHPLEIFEVWHGGMSFHGG
                     FLGVLIALWFFSRRRNKSLLDVTDFVAPLCPLGLAAGRLGNFINGELVGRPTDVPWAM
                     VFPQVDQVPRHPSQLYEFGLEGVLLFIVLWIFASRRRPRGAVSGLFLVGYGTFRFIAE
                     FAREPDNFLGFLAGGLTMGQWLSMPMIVIGALMMLFAYRRARKDQPTLLDRMKG"
     gene            2372..4054
                     /gene="ilvD"
                     /locus_tag="VFO53_06585"
     CDS             2372..4054
                     /gene="ilvD"
                     /locus_tag="VFO53_06585"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET8875980.1"
                     /translation="MPQNRRSRLITEGIARSPNRAMLRAVGFGDADFDKAIVGVANGH
                     STMNPCNAGIQPLVDRAMKALHEAGAMPQVFGVPTVTDGIGMGTEGMKYSLVSREVIA
                     DAIETAVNGQCMDGVLIVGGCDKNMPAGMMAMARMNVPGIYVYAGTIKPGHWKGQTLT
                     IVSPFEAVGAFTAGKLAKEDYDGIERNACPTVGACGGMYTANTMSSSFEALGMSLLGS
                     SQLASPDREKADSSAESAQVLVRAIAQDLKPRDIITRRAIENAISLVMATGGSTNAVL
                     HYLAIAAAAEVEWTIDDFERIRKRVPVLCDLKPSGRYVAVDFNAAGGVPQVLRMLLDA
                     GLLHGDCMTITGRAIGDELADVSSSPRPDQDVIRPLDKPLYAHGHLAILRGNLAPEGC
                     VAKITGLKNPAITGPARVFDSEPACMEAILARKIHPGDVIVIRYEGPKGGPGMQEMLA
                     PTSALVGQGLGESVGLVTDGRFSGGTWGMVVGHVAPEAYVGGTIALVEEGDPVTIDAH
                     RLLIELGVDDETLAARRARWQPPPPRYTRGLLAKYMRLVSTASLGAITDSPA"
     gene            4176..4898
                     /locus_tag="VFO53_06590"
     CDS             4176..4898
                     /locus_tag="VFO53_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875981.1"
                     /translation="MSPQATVRIAALMLAALVAGCAGLDQHGDLAGRVAAAPTLVIGQ
                     SWTYRGREGFRAPLVWEETHEVTAIRPDGIAVRVTASGDRVSGERTELLASPGIVRVG
                     ALMDIETRRFATPLDRFRFPLAPGETWNQWVDNFNETTNKAGQINRYVRVGGWERVST
                     PAGVFDAIRMRVMMRLDDEEFWRYPTTCNYLIWYAPAVGAPVREEKEAEYYEKGQEFD
                     GFGAIRSQHTLIELVAYAPGRG"
     gene            complement(4903..6402)
                     /locus_tag="VFO53_06595"
     CDS             complement(4903..6402)
                     /locus_tag="VFO53_06595"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET8875982.1"
                     /translation="MRRAIGALIVALTALACCSSESVFAAALAYIPNAEQAGVVTVLD
                     TDTNVVAAVIPAGNVPVGIAIAPDGTRIYVSNLYQERGYGVGTVSVISAQPAALLATV
                     PVGNQSTGIAVTPNGRFVYVANSSDNTVSVIEAATLTVMATIAVGSQPEGVVASPDGG
                     RVYVAAYRNVYVISTGTNVVAKKIPTSDFANGVALSRDGRRLYVANINTDVLDGRGSV
                     SVISTETDTVMATIAVGVHPNGVAISPDAARVYVANSSDSTVSVIDTAVNAVSATVRV
                     GNFPFGVSVTPDGSRVYVANNGESTVSVVDTRSNQVVETIHIANGVIAFGNFIGPELS
                     PQTSPVVEFHNAALDHYFITQAADEIAGLDGSVHPGWARTGQTFLAWLPGRSSGNAKP
                     VCRYYGLPSAGLDSHFYSASVAECVAVAERFGSAWTKEADDVFEIGLPDATSGACSDG
                     ATPVYRLWNGRADSNHRYTTDAASRQHMIDLGWVPEGYGPLGVVMCAPH"
     gene            complement(6409..>7052)
                     /locus_tag="VFO53_06600"
     CDS             complement(6409..>7052)
                     /locus_tag="VFO53_06600"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET8875983.1"
                     /translation="APHVSCIGSTRDGIRQVLARYREHGIRHLVALRGDLPSGSQDVG
                     DFRYANELVRFIREETGDWFHIDVAAYPEVHPQARNPEDDLASFQRKIDAGADSAITQ
                     YFFNTDAYWHFVDACSRRGVNVPIVPGIMPIGSFSKLARFSDACGAEIPRWIRRRLEG
                     LGDDSASIRAFGLDVVTTMCASLIERGAPGLHFYTLNQPTLTTAIWRALGLGK"
BASE COUNT         1260 a         2291 c         2341 g         1160 t
ORIGIN      
        1 gctgctggcc ctgcagatcg gggagcacga gaccgagcac cgccgagccg tcgcggctgg
       61 cgtcgttggc gtcgtggacg aaatacgccg agccggcgcc cagagcgagc gccgccgctg
      121 caaccgcacc gacgatcagc agccgccggc gtggccgtgc attcgatggc gcagccggag
      181 atgcgccaag cggcgggatg gctctcactt gaaccatccc ttgcttcccg cctcgacgaa
      241 ggcgcccggc ttgccgccca gcgggatcgc aagcgtgagc tgctgtggat tggggggata
      301 gcagacgccg acatcggcgc agccttgcga attggcgtgc aacgtgatcg tcttgcctgc
      361 ggccgcttgc gcgagcggca cccggacgac cacctcgccg cggtacgtct cgacatcgcc
      421 aaaaaacgca tcgtgcttgg gcttgccggg cggcaggtcg ggcttcacgg caggaacagg
      481 ctcggtggtg aactgcattt tgtcgcgata caggtaatag ccgtcggcga cgtcgaagcg
      541 cgcctcgatc gtgctcgcgt cgagcgcgcg cgctgacagg tgaaatgcgc gctccggcgg
      601 gaggagcttc ggcgcaagcg acgcaatgcc ctcggcgtgc gccgttgcga acgcgattgc
      661 cagcagcggc acgagcgagc gcgcccggcg gtcagctcgg ggccgtctcg gcggcgatcc
      721 agtcgagata tcgcgcctcg ccgccgacga tgggaattgc gatgatctcg ggcgtgtcat
      781 agggatgggt tttgcggatg acgtcctcca cgttgtaata caacgcggaa cgcgtcttga
      841 tggcgagtgg aacctcggtt gccgtttcga ttcctccgcg ccagtgataa attgagtcca
      901 ctgcagcgcc gatgtttacg cacgccgcga gccgatcgtt caaaagcgcg ttcgccagtt
      961 ccgtcgcgac ggcatggtcg ggcgcatgcg tgacgacgag gatcgccgcg tcgcgagaag
     1021 gagtcgtaat gcgtttcctt atgctggcca ttgtactgat attcccgctg gtggacctcg
     1081 tggtcaccgc aaggttcgcc cgctggacgg gcgtacccat gtggatgtgg cttgcgggct
     1141 cggcggttgc aggtttgtgg gtactcgcta acgagcgggt gcagttccgc tcgcgcgccc
     1201 tcgccgcgtt tcgccgcgac gcgctgctgc gggggctcct cgacagcggc cggcgcgtcc
     1261 ttgccggggt gttgctgatc gcgcctggcc tgctgagcga cgtgctcgca ttgctgctgc
     1321 tcctgctgcc gatcaaccag cgtggcgcct tcggcggtcc ccagcccgtg gcggccggcc
     1381 gtgcgcccta ccggagcgac cgcacgctcg acggcgacta tcggcgcatc gactagccgt
     1441 ccagacgtcg gtccgagcgg gctcgacctc gcgcttcatc ccttcatccg gtcgagcagc
     1501 gtcggctggt ccttccgcgc gcgccggtag gcgaacagca tcatcagcgc gccgatcacg
     1561 atcatcggca tcgacagcca ctgacccatc gtcaggccgc cggcgagaaa gcccaggaaa
     1621 ttgtcgggct cgcgcgcgaa ttcggcgatg aagcggaacg tgccgtagcc gacgaggaac
     1681 aggccggaca ccgcgccgcg cggccgccgg cgtgaggcga agatccatag cacgatgaag
     1741 agcagcacgc cttcgagccc gaactcgtaa agctgcgacg ggtgtcgcgg cacctggtcg
     1801 acctgcggga agaccatggc ccacggaacg tcggtcggcc ggccgacgag ctcgccgttg
     1861 atgaagttgc ctagccgccc ggcggccagg cccagcgggc acagcggcgc gacgaaatcg
     1921 gtgacgtcga ggagcgactt gttccgccgc cgcgaaaaga accacagcgc gatcagcacg
     1981 ccgaggaagc cgccgtgaaa gctcatgccg ccgtgccaca cctcgaagat ctcgagcgga
     2041 tgcgcgaagt agtagagcgg cttgtagaac aacacgtaac cgagccggcc gccgaggatc
     2101 acgccgaaca cgccgaagaa cagcatgtcg tcgacatcgg acattcgcca tccggtgagc
     2161 atttcggcgc gtgcacgccg gcgcccgagc acgatgaaca ggatgaatcc ggcgaggtac
     2221 atgagcccgt accagcgaac cgcaagcggg ccgacgtgaa acgcgacggg atcgaaatcg
     2281 ggatggacga gcatcggggt cgatatatca caaaccgggc gaccggtatc attgacggct
     2341 tcccgcttgc cgttcgcatg gagattcccg cgtgccgcag aaccgccgct cccgcctgat
     2401 taccgagggc atcgcccgct cgcccaaccg cgcgatgctg cgtgccgtcg gcttcggcga
     2461 tgccgatttc gacaaggcga tcgtcggcgt cgccaatggc cacagcacga tgaatccgtg
     2521 caacgcaggc atccagccgc tggtcgatcg cgccatgaag gcgctgcacg aggccggcgc
     2581 gatgccgcag gtgttcggcg tgccgacggt caccgacggc atcggcatgg gcaccgaagg
     2641 catgaagtat tcgctcgtgt cgcgcgaggt gatcgccgat gcgatcgaaa ccgccgtcaa
     2701 cggccaatgc atggacggtg tgctcatcgt cggcggctgc gacaagaaca tgccggcagg
     2761 catgatggcg atggcacgga tgaacgttcc gggcatctac gtctacgcgg gaacgatcaa
     2821 gccgggacac tggaagggac agacgctgac gatcgtctcg ccgttcgaag cggtcggcgc
     2881 gttcaccgcc ggcaagcttg cgaaggagga ttacgacggc atcgagcgca acgcgtgccc
     2941 gaccgtgggc gcatgcggcg gcatgtacac ggcgaatacg atgtcgtcgt cgttcgaggc
     3001 actcggcatg agcctgctcg ggagctcgca gctcgcgtca cccgatcgcg agaaggccga
     3061 ttcgtcggcc gagtcggcgc aggtgctcgt gcgcgcgatc gcgcaggact tgaagccgcg
     3121 cgacatcatc acgcgccgcg cgatcgaaaa tgcgatctcg ctcgtcatgg ccaccggcgg
     3181 gtcgaccaac gcggtgctcc attacctggc gatcgccgcg gccgccgagg tcgagtggac
     3241 gatcgacgat ttcgagcgca tcagaaaacg cgtgcccgtg ctgtgcgacc tgaagccttc
     3301 gggccgctac gtcgcggtcg acttcaacgc cgcgggcggc gtgccgcagg tgctgcggat
     3361 gctcctcgac gcggggctcc tccacggcga ttgcatgacg atcaccgggc gagcgatcgg
     3421 cgacgagctt gccgacgttt cgtcttcgcc acgtcccgat caggatgtga tccggccgct
     3481 cgacaagccg ctttacgcgc acgggcatct cgcgatcctg cgcggcaatc tcgcgccgga
     3541 aggctgcgtc gcgaagatca ccgggctcaa gaaccccgcg atcaccggtc ctgcacgcgt
     3601 gttcgattcc gagcccgcct gcatggaggc catcctcgcg cgcaaaatcc atcccggcga
     3661 tgtaatcgtg atccgctacg aaggtccgaa gggcggcccg gggatgcagg agatgctggc
     3721 gcccacttcc gcgctggtcg gacagggcct cggcgaatcg gtcgggcttg tcaccgacgg
     3781 ccgcttctcg ggcggcacgt ggggcatggt cgtggggcac gtggcacccg aggcgtacgt
     3841 cggcggcacc atcgccctcg tggaggaagg cgatcccgtc acgatcgacg cgcatcgcct
     3901 gctgatcgag ctcggcgtcg atgacgagac gctcgccgca cggcgggcgc gctggcagcc
     3961 gccgccgccg cggtatacgc gcggactgct cgcgaagtac atgcggctcg tgtcgacggc
     4021 gagcctcggc gcgatcaccg attcgcccgc ctgacgctcg gcggcagcga cggaaccccg
     4081 tcgccgcttc gttgcccatg ccgcaacaca ccgcggtgga agatgcgcac ctgcgttcct
     4141 gttcgccgtt gatctgaccg gagtccgaga atcccatgtc gccacaggcc accgtccgca
     4201 tcgcagcgct catgctcgcc gcgctggtcg ccggctgtgc cggactcgat cagcacggcg
     4261 atctcgccgg tcgcgtcgcc gcggcgccga cgctggtcat cgggcaaagc tggacttacc
     4321 gcggtcgcga aggtttccgc gcgccgctcg tatgggagga aacgcacgag gtgaccgcga
     4381 tccggccgga tggcattgcc gtgcgcgtga cagccagcgg cgatcgagtc agcggcgagc
     4441 gcaccgagtt gcttgcatcg cccggcatcg tgcgcgttgg cgcactcatg gacatcgaga
     4501 cgcgacgctt cgcaacgccg ctcgatcgct tccgcttccc gctcgcgccg ggcgagacct
     4561 ggaaccagtg ggtcgacaac ttcaacgaga cgacgaacaa ggccgggcag atcaaccgct
     4621 acgtccgcgt cggcggctgg gaaagggtat cgacgccggc cggcgtgttc gacgcgatcc
     4681 gcatgcgcgt gatgatgcgg ctcgacgacg aggagttctg gcgctacccg acgacgtgca
     4741 actacctgat ctggtacgcg cccgcggtgg gtgcgccggt gcgcgaggaa aaggaagcgg
     4801 agtactacga gaagggccag gagttcgacg gtttcggcgc catccgctcg cagcacacgc
     4861 tgatcgagct cgttgcgtat gcgccgggac gcggctgacg actcagtgcg gcgcgcacat
     4921 cactacgcca agcggcccat acccttccgg gacccacccg agatcgatca tgtgctgacg
     4981 gctggccgcg tccgtcgtgt agcggtgatt tgaatcggcc cgcccgttcc acaggcgata
     5041 taccggagtc gcgccatccg aacaggcacc gctcgtcgca tccggcaagc cgatctcgaa
     5101 cacgtcgtcc gcttccttcg tccaggcgct gccgaagcgc tcggccaccg cgacgcactc
     5161 tgcgacgctg gccgagtaaa agtgcgaatc gagcccggcg gaaggcaaac catagtaacg
     5221 gcataccggt ttggcgttgc cgctcgatcg gccgggcaac cacgcaagaa atgtttgtcc
     5281 ggtgcgcgcc cagcccggat gcaccgatcc atcgaggcca gcgatctcgt cggcagcttg
     5341 cgtgatgaaa tagtgatcga gcgcggcgtt atggaactcg acgaccggcg acgtttgcgg
     5401 agacagttct gggccgatga aattgccgaa cgcaataacg ccattcgcga tgtgaatcgt
     5461 ttccacgacc tgattgctcc tggtatcgac gacggaaacg gttgattcgc cgttgttagc
     5521 gacatagacc cgtgaaccat cgggtgtcac ggaaacgcca aacggaaaat taccgacacg
     5581 caccgtggcg ctgaccgcgt tcacagccgt gtcgatgacg gataccgtgc tatcggacga
     5641 gttcgccacg tagacgcgtg ccgcatcggg agagatcgca actccgttcg gatgcacacc
     5701 aaccgcaatg gtggccatga ccgtgtccgt ttcggtagag atgaccgaaa cgcttcctcg
     5761 cccatcgagg acatcggtat tgatattggc cacgtacagc cgtcgtccat cgcgactgag
     5821 cgctacgccg ttggcgaagt cgctggtcgg tattttcttt gccacgacat tcgtgccggt
     5881 cgaaatgacg tagacgttcc tgtacgctgc gacgtagacg cggccaccgt cgggcgaagc
     5941 taccactcct tccggctgcg agcccaccgc gatcgtcgcc ataacggtca gcgtggcggc
     6001 ctcgatcacc gatacggtgt tgtccgacga gttcgcgaca tagacgaatc ggccgtttgg
     6061 cgtcactgca atgcccgtcg attgattacc gacaggaacg gtcgcgagaa gcgccgccgg
     6121 ttgcgcggag atgacggaaa ccgttccgac tccgtagccg cgctcctgat agagatttga
     6181 cacgtatatt cgcgttccgt cgggcgctat cgcgatgcca accggcacgt ttccggcagg
     6241 aataacggcg gcgaccacgt tcgtgtccgt atcgagcacg gtcacgactc ccgcctgctc
     6301 ggcattggga atgtacgcga gagcggccgc aaacacgctc tcgctgctgc agcacgcaag
     6361 cgcggtcagg gccacgatca atgcgccgat cgcccgacgc atcgctcgct actttcccaa
     6421 tcctagtgcg cgccagatcg ccgtcgtcag cgtcggctgg ttcagcgtat agaagtgcag
     6481 cccgggtgcg ccgcgctcga tcagcgacgc gcacatcgtc gtgacgacgt cgagcccgaa
     6541 cgcacggatc gatgcgctat cgtcgccgag cccttcgaga cgccgacgaa tccagcgcgg
     6601 tatctcggcg ccgcacgcat cggagaagcg cgcgagtttc gagaagctgc cgatgggcat
     6661 gatgcccggc acgatgggca cgttcactcc gcgacgcgag caggcatcga cgaagtgcca
     6721 gtaggcatcg gtgttgaaga agtactgcgt gatcgccgaa tcggcgcctg catcgatctt
     6781 gcgctggaag ctcgcgagat cgtcctcggg gttgcgcgcc tgcggatgca cctcgggata
     6841 cgcggccacg tcgatgtgga accagtcgcc ggtttcttcg cgaatgaagc gcacgagctc
     6901 gttcgcatag cggaaatcgc ccacgtcctg gctgccggaa ggcagatcgc cgcgcaacgc
     6961 gacgaggtgg cggatgccgt gctcgcgata tcgggccagc acctgacgga ttccgtcgcg
     7021 cgtcgagccg atgcacgaga cgtggggcgc ac
//
LOCUS       DASSVX010000149         3401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_205672, whole genome shotgun sequence.
ACCESSION   DASSVX010000149 DASSVX010000000
VERSION     DASSVX010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205672"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VFO53_06605"
     rRNA            complement(<1..178)
                     /locus_tag="VFO53_06605"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(667..1581)
                     /locus_tag="VFO53_06610"
     CDS             complement(667..1581)
                     /locus_tag="VFO53_06610"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875984.1"
                     /translation="MRHALKFLTRLPLPVLYGLGGFAYFVAFHVLRWHRELAARNLAN
                     AFPEKPPAERAALLRQSFVNLGQTLAETFWGYGATAEELAKRVAIENPELVARFVRES
                     RPVVVLTAHVCNWEWLLPATSSQFGIPIDAVYKPLRVASLDAFIRETRSRFGGKPIPI
                     QSFLFELMRRAGDARAYAMVADQTPPRHMDKHWTRFLNQDSAFFTGADKIARFLDAPV
                     LYVAMRRVSKGHYSVRLHVLVEPPYDGDDDLQVVERYARRLETEIRNNPADWLWVHNK
                     WKYPRPASTPEDGGTRARRRTQQRTSAP"
     gene            1782..2357
                     /locus_tag="VFO53_06615"
     CDS             1782..2357
                     /locus_tag="VFO53_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HET8875985.1"
                     /translation="MSAAEHARAARFGSPLLRDRYVVGRAALRTVLAEVLGVSPGSVA
                     IVRGPRGRPQLDDGAPLDFNVSHTGDVALIGVTRQGRVGVDVERADRSINVPGIARKF
                     LTHTERHRLATLDSDAARRDVLRLWTCKEAMSKATGDALSAPFAAIDIDLRDGGLALR
                     DGPGKYRPAGWALHAVEMPGDYLATVALWRG"
     gene            complement(2378..3193)
                     /locus_tag="VFO53_06620"
     CDS             complement(2378..3193)
                     /locus_tag="VFO53_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET8875986.1"
                     /translation="MLRSYLFVPGNRPDRYAKACATRAGAVIVDLEDAVAPAEKAAAR
                     DALAAWLSAQRRVLVRVNAPGSPWFADDLRACASDGVSAIVLPKAERAEDVEQVAAIC
                     GSRPVLPLVETARGVWNVLAVAQAPHVRSLLFGSLDFQSDLGTSDDDLLYARTRLVLA
                     SRVAGIGAPVDGITQAIDDPELLRRDCERARQLGFGGKLCIHPKQVDIVNRCFSPSAD
                     DLAWARRVIDAFAQSSGNAALLDGRMIDRPVLVRAQALLGDAGEGAGRESSGR"
BASE COUNT          571 a         1178 c         1122 g          530 t
ORIGIN      
        1 tagagcgtat gcggtattag cgccgctttc gcggcgttat cccccactac gggacacgtt
       61 ccgatgcatt actcacccgt tcgccactga agtggagttg cccccacccc cgttcgactt
      121 gcatgtgtaa agcatgccgc cagcgttcaa tctgagccag gatcaaactc ttcagttcga
      181 tcttcaatgc aaagttccaa ctcacttgac tctgctgccc acccccttga cgagaacgga
      241 cagcgcacgg ccagccgccg cgcactcgcg tacgcaaacc gccaaccgca ctagcgtcgt
      301 gtgagcactc gagcttggga attcccccca gctacccgca cccggcgttc gcacaccagg
      361 caccggcacc cgaggacccc gcctcagtac ccacacctat cgattgctga ttgttaaaga
      421 gcgacttcgc tacatggggc cgctttggat tgcttcaagc gacccgctcc cgttgtcatg
      481 caattgcgct gtgggagttt tgcagcggag gacagaaatt atacgacttc ggcctacaca
      541 atgcaagctc gatgtgcact gttcgcgtgt cgcaacgcaa cgccggattt gcttttacga
      601 accgccgcgc ggttcgccga accgcgcatt attgctcgga gcaaatggtg ccggaggttg
      661 cccgcgtcaa ggcgccgacg tccgttgctg cgtgcgtcgc cgcgcacgcg tgccgccgtc
      721 ctccggcgtg ctcgcggggc gcggatattt ccacttgttg tgcacccaga gccaatcggc
      781 cggattgttc cggatctcgg tctcgagccg gcgcgcgtac cgctccacga cctgcagatc
      841 gtcgtcgccg tcgtacggcg gctcgacgag cacgtgcagg cgcacgctgt agtggccctt
      901 gctcacgcgg cgcatcgcga cgtagagcac gggcgcgtcg agaaagcgcg cgatcttgtc
      961 cgcaccggtg aagaatgcgc tgtcctggtt gaggaagcgc gtccagtgct tgtccatgtg
     1021 tcgcggcggt gtctggtccg cgaccatcgc gtacgcgcgc gcatcgccgg cgcggcgcat
     1081 cagctcgaac aggaacgact gaatcgggat cggcttgccg ccgaagcggc tcctcgtctc
     1141 gcggatgaac gcgtcgaggc tcgcgacgcg cagcggcttg tacaccgcgt cgatcggaat
     1201 cccgaattgc gagctcgtcg ccggaagcag ccactcccag ttgcacacgt gcgcagtcag
     1261 cacgacgacc ggccggcttt cgcggacgaa gcgcgcgacc agctcggggt tctcgatcgc
     1321 gacgcgcttc gcgagctcct ccgccgtcgc gccatagccc cagaacgttt ccgcgagcgt
     1381 ctgcccgagg ttgacgaagg attgacgcag caacgccgcg cgctccgccg gcggcttttc
     1441 cggaaatgcg ttggcgaggt ttcgcgcggc gagctcacgg tgccagcgca acacgtggaa
     1501 cgcgacgaaa taggcgaagc cgccgaggcc gtagagcacc ggcagcggta agcgcgtgag
     1561 aaacttgagg gcatgacgca tacgaacgcg cccgcgtgga agcgggcgca aggctcgaac
     1621 ggggtgtctc tttgagctta gacgagaacc gtggcgcggg caacgccggc gtcgcgcaag
     1681 tcccgaacgt ggccgcgctg gcgtcgcccg caccgggagt gcgcctctgg cggtgcgcgc
     1741 ttcacgccac gtcgtcgcag ctgcgatcgt gtgcgagcgc gttgtccgcc gccgagcacg
     1801 cgcgcgcggc gcggttcggc agtccgctgt tgcgcgaccg gtacgtggtc gggcgtgccg
     1861 ccttgcgcac cgtgcttgcc gaggtgctcg gcgtcagccc cgggagcgtc gcgatcgtgc
     1921 gcggcccgcg cggacgcccg cagctcgacg acggtgcgcc gctcgacttc aacgtctcgc
     1981 acaccggcga tgtcgcgctg atcggcgtaa cgcgccaggg tcgcgtcggt gtcgacgtcg
     2041 agcgtgccga tcgcagcatc aacgtgccgg gcatcgcgcg caagttcctc acgcataccg
     2101 agcgccatcg gctcgcaacg ctcgattccg atgccgcgcg ccgcgacgtg ctgaggcttt
     2161 ggacgtgcaa ggaagccatg agcaaggcga ccggggacgc gctgtccgcg ccgtttgccg
     2221 cgatcgacat cgacttgcgc gacggcggct tggcgctgcg cgacggaccg ggcaaatacc
     2281 ggccggccgg ctgggccctg cacgcagtgg aaatgccagg cgattacctc gccacggttg
     2341 ccttgtggcg cggttgaagc ggtcgccgtc gggcgcttta ccggcccgac gactcccggc
     2401 ccgcgccttc gcccgcgtca ccgagcagcg cctgcgcccg cacgagcacc ggccggtcga
     2461 tcatcctgcc atcgagcaac gcggcattgc cgctcgactg cgcaaaggcg tcgatgacac
     2521 gccgcgccca ggcgaggtcg tccgccgacg gcgagaagca ccggttcacg atgtcgacct
     2581 gcttcggatg aatgcagagc ttgccgccga agccaagctg gcgcgcccgc tcgcaatcgc
     2641 gacgcagcag ctcgggatcg tcgattgcct gcgtgatgcc atcgaccggc gctccgatcc
     2701 cggcaacccg tgacgcaagc acgagccggg tgcgtgcata cagcaggtcg tcgtcgctcg
     2761 tgccgaggtc ggactggaaa tcgagtgagc cgaagagcag cgagcgcacg tgcggcgcct
     2821 gtgccacggc cagcacgttc cacacgccgc gcgccgtctc gacgagcggc agcacgggcc
     2881 gcgaaccgca aatggcagcc acctgttcga cgtcctccgc gcgttcggcc ttgggcaaca
     2941 cgatcgcgct gacgccgtcg ctggcgcacg ctcgcagatc gtcggcaaac cacgggctgc
     3001 ccggcgcgtt cacacgaacc agcacgcgcc gctgcgctga aagccatgcg gccagcgcgt
     3061 cccgggccgc ggccttctct gccggcgcga cggcgtcctc gagatcgacg atcaccgcgc
     3121 cggcgcgcgt cgcgcaggcc ttcgcgtagc gatcgggccg attgccgggc acgaacaggt
     3181 aggaacgaag catcgcaaga ggcacccgcg aacgccgccg tgcgggcgat ataccgaaag
     3241 gtaaagcggc acacgatcgg ggaaatcggc catgcggtca agcacccggg gcgctgggcg
     3301 agaagcacgt cggagcaagg cggcacgccc gaccacgtac cggtcgcgca acagcggact
     3361 gccgaaccgc gccgcgcgcg cgtgctcggc ggcggacaac g
//
LOCUS       DASSVX010000150         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_688980, whole genome shotgun sequence.
ACCESSION   DASSVX010000150 DASSVX010000000
VERSION     DASSVX010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688980"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VFO53_06625"
     CDS             <1..1025
                     /locus_tag="VFO53_06625"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylornithine/succinyldiaminopimelate
                     transaminase"
                     /protein_id="HET8875987.1"
                     /translation="AQARTLWHVSNWFTNEPALRLARRLVEATFAERVFFCNSGAEAN
                     EAALKLARRYAHDRSGPQRIRVVAAQNAFHGRTLFTVTAGGQPKYSSGFGPNPAGITH
                     VRYNDIAALEAEFAAHGNEICAVLLEPMQGEGGMTPGTPEYLQAARRLCTAHGALLIL
                     DEIQSGMGRTGALFSYMQKGIVPDIVTSAKGLGGGFPIGAMLTTTDIASAFSVGVHGT
                     TYGGNPLACAVAGAVFDVVNTTEVLDGVKARHALFTEGLRAINGRRQVFRDVRGEGVW
                     IGCELAETWRGKAFDVMTAAGEAGLLVLMAGPDVVRIAPSLVISLDEILEGLARLETA
                     LNRALV"
     gene            1032..2084
                     /gene="astA"
                     /locus_tag="VFO53_06630"
     CDS             1032..2084
                     /gene="astA"
                     /locus_tag="VFO53_06630"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HET8875988.1"
                     /translation="MFFIRPIARDDLPVVLALSERTGTGLTTLPADQDRLLQRIDRSL
                     ASFAGTADKADACYMFVLVETGNATPPRVVGISAIEAAVGLREPWYNYHVGTLVHASR
                     ALDVYTVSPTLFLSNDHTGHTELCSLFLDQRYRMGKNGPLLAKSRLMFMAEFAELFAP
                     KVIAELRGRLEADGSSPFWEGLGRHFFAMEYSTADYLTGLGQKSFIAELMPRHPVYVN
                     LLPPSAREAIGAVHADTAPARAMLEQEGFRYEGYVDIFDAGPTVECARENIDLVRRSA
                     VMTCTLGEEDPVPDSLTDDILWLVCNRRFDTFRALVAAAPARVDRFPLLPYAAEALGV
                     TEGDSVRAVPLAPADR"
     gene            2098..>2853
                     /locus_tag="VFO53_06635"
     CDS             2098..>2853
                     /locus_tag="VFO53_06635"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010626670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HET8875989.1"
                     /translation="MNFDGLPGPTHNYSGLAHGNLAAERNALQVANPREAALQGLAKM
                     RALATRGFAQGVLPPHERPSVDALRAIGFSGRDHEVIAAAARDAPELLAACSSAAAMW
                     VANAATVSAAADCADGRVHFTPANLATNFHRSLEPATTTRMLRAIFADDTRFEVHDAL
                     PATPQFGDEGAANHTRFAAGIGEPGVELFVYGRRGFGGGTGPSRYPARQTREASEAVA
                     RRHGLDPVRTLFAQQRPDSIDAGVFHNDVIAVGE"
BASE COUNT          467 a          948 c          941 g          497 t
ORIGIN      
        1 atgcgcaggc gcgcacgctg tggcatgtgt cgaactggtt cacgaacgag cctgcgctgc
       61 ggctcgcgcg acggctcgtc gaggcgacgt ttgccgagcg cgtgttcttc tgcaattccg
      121 gtgcggaggc gaatgaagcg gcgttgaagc ttgcgcgccg ctatgcgcac gaccgttcgg
      181 gcccgcagcg cattcgcgtc gtggcagcac agaacgcatt ccatggccgc acgctgttca
      241 cggtaacggc cggcggtcag ccgaagtatt cgtccggttt cggtcccaac cctgcaggca
      301 tcacgcacgt tcgctacaac gacatcgcgg cactcgaagc ggaatttgcc gcgcatggca
      361 acgagatctg cgcggtgctg ctcgagccaa tgcagggcga aggcgggatg acgccgggca
      421 cgcccgaata tctgcaagcg gcacgacgac tgtgcacggc gcatggcgcg cttctgatcc
      481 tcgacgagat acagagcggc atggggcgca ccggcgcgct gttctcgtac atgcagaagg
      541 gcatcgtccc cgacatcgtc acgagcgcga agggattggg gggtggattc ccgatcggtg
      601 cgatgctgac gacgaccgac attgcgagcg cattctcggt tggtgttcac ggaacgacgt
      661 acggcggcaa tccgcttgcg tgcgccgtcg ccggcgccgt gttcgacgtc gtcaatacga
      721 ccgaggtgct cgatggcgtc aaggcgcgcc acgcgctgtt cactgaagga ctgcgcgcga
      781 tcaacggtcg gcgacaggtg tttcgcgacg tgcgcggcga aggcgtatgg attggttgcg
      841 agcttgcgga gacctggcgc ggcaaagcgt tcgacgtgat gaccgccgca ggcgaggctg
      901 ggctgctcgt gctcatggcg ggcccggacg tcgtccgcat cgcgccgtcg ctcgtcatct
      961 cgctcgacga aatcctcgaa gggctggcgc gcctcgaaac cgcgttgaac cgcgcgctgg
     1021 tgtaagcggc gatgtttttc atccggccga tcgcgcgcga cgatctgccg gtcgttctcg
     1081 cgctctccga gcgcaccggc acggggctca ctacacttcc cgccgaccag gatcgattgc
     1141 tgcagcggat cgatcgctcc ctcgcatctt ttgccggcac cgccgacaag gccgacgcct
     1201 gctacatgtt cgtgctcgtc gagaccggca atgcgactcc gccgcgcgtc gtcggcatca
     1261 gtgcgatcga ggccgccgtg ggactgcgcg agccctggta caactatcac gtcggcacgc
     1321 tcgtccatgc atcgcgagca ctcgatgtct acacggtctc cccgacgctg ttcctgtcga
     1381 acgaccacac cggtcacacc gagctctgct cgctgttcct cgatcagcgc tatcggatgg
     1441 gcaagaacgg cccgctgctc gcgaaatcac ggctgatgtt catggcagag ttcgcggagc
     1501 tcttcgcccc caaggtgatc gccgagcttc gcggccgcct cgaggccgat gggtcgagcc
     1561 ccttctggga aggtcttggg cggcacttct tcgcgatgga atactcgacc gccgattacc
     1621 tgacgggcct cggtcaaaag tcattcattg ccgagctgat gccgcgccat cccgtctacg
     1681 tgaacctgtt gccgccttcc gcgcgcgaag cgatcggcgc cgtgcatgcc gatacggcgc
     1741 ccgcgcgcgc aatgctcgag caggaaggat ttcgctacga gggctacgtc gacatcttcg
     1801 acgccggacc gacggtcgaa tgcgcgcgcg agaacatcga tctcgtgcgc cgctcggcgg
     1861 tgatgacgtg cacgctcggc gaggaagatc cggtacccga cagcctgacg gacgacatcc
     1921 tgtggctcgt ctgtaatcgt cgcttcgaca cgtttcgcgc gctcgttgcc gcggcgcccg
     1981 cacgcgtcga ccgctttccg ttgctgccgt acgcggcgga ggcactgggc gtcaccgaag
     2041 gcgacagcgt gcgcgcggtt ccattggcgc ccgcggatcg ctgaagatat tcacgtgatg
     2101 aatttcgacg gactgcccgg gccaacgcac aactattcgg gactcgcgca cggcaacctc
     2161 gccgccgagc gcaacgcact gcaggtcgcg aatccgcgcg aggccgcgct gcagggactc
     2221 gcgaagatgc gcgcgctcgc gacgcgcggc ttcgcgcagg gtgtcttgcc accgcacgag
     2281 cggccgagcg tcgacgcgct tcgcgcgatc ggcttttccg gtcgcgatca tgaagtcatt
     2341 gccgcagcgg cgcgtgacgc gcccgagctg ctggcagcat gctcgtcggc agccgcgatg
     2401 tgggttgcga acgcggcgac cgtcagcgct gcagccgatt gcgcggacgg gcgagtgcat
     2461 ttcacgccag cgaacctcgc gacgaacttt catcgctcgc tcgagccggc aacgacgacg
     2521 cgaatgctgc gcgctatctt tgccgacgat acgcgcttcg aagtgcacga tgcgcttccc
     2581 gccacgccgc agttcggcga cgaaggtgca gcgaatcaca cgcgtttcgc ggcggggatt
     2641 ggtgagccag gcgtcgagct gttcgtttac ggtcgacgcg gctttggcgg cggcacaggg
     2701 ccctcccgct accccgcgcg gcaaacgcgc gaggcgagcg aagccgtcgc gcgccgccac
     2761 ggcctcgatc ccgtccgcac gctcttcgcg cagcaaaggc cggacagcat cgacgccggc
     2821 gtcttccaca acgacgtcat tgccgtcggc gaa
//
LOCUS       DASSVX010000151         4620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_688987, whole genome shotgun sequence.
ACCESSION   DASSVX010000151 DASSVX010000000
VERSION     DASSVX010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688987"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..687
                     /gene="greB"
                     /locus_tag="VFO53_06640"
     CDS             109..687
                     /gene="greB"
                     /locus_tag="VFO53_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HET8875990.1"
                     /translation="MSKAFTNEEAAESDDGEIVEPSPLPAGARNYMTPGGFARMRREL
                     DTLVAKERPELVATVAWAAGNGDRSENGDYIYGKKRLREIDRRIRFLVRRLDAAEVVD
                     PAMPRDDDAASRVYFGATVTIADASGGERTVSIVGIDEIDTARGYISWISPMARALLR
                     ASEGDTVSVHTPGGIEELDVVGVRYVALATDP"
     gene            737..931
                     /locus_tag="VFO53_06645"
     CDS             737..931
                     /locus_tag="VFO53_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875991.1"
                     /translation="MDADLAALEERLDTLIAYARELRAANELLRSELLASQERNRDLT
                     ARVTQATERLDALIARMPVE"
     gene            951..1310
                     /locus_tag="VFO53_06650"
     CDS             951..1310
                     /locus_tag="VFO53_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET8875992.1"
                     /translation="MTQNAARTVTLDVTILGRDFKVACKEDERDELLEAVALLDRRMR
                     EIRDAGKVSGTDRIAVMAGLNIAHELLRERRASAIAVDARPATGGESFDAEAARRRIQ
                     AMQATIDETLAGQENLF"
     gene            1359..1537
                     /gene="ssrS"
                     /locus_tag="VFO53_06655"
     ncRNA           1359..1537
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFO53_06655"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1630..2091
                     /locus_tag="VFO53_06660"
     CDS             1630..2091
                     /locus_tag="VFO53_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HET8875993.1"
                     /translation="MRRYWLMKSEPDEFSIDDLVAAPSQTTPWVGVRNYQARNFMRDG
                     MRVGDRAFFYHSSCPEPGVAGVVEIARRAYPDQTQFDPKSAYYDPKSPRDAPRWVHVD
                     VKLVRKTRLVTLPMLRATPGLEDMVVLRRGNRLSITPVTPAEWKIVEKLAK"
     gene            2088..2897
                     /locus_tag="VFO53_06665"
     CDS             2088..2897
                     /locus_tag="VFO53_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET8875994.1"
                     /translation="MTDWMLLPVLLAFGGLVGFLAGLLGIGGGMTLVPLLTILFTARD
                     FPADHIIHMAVATATATMVFTAMSSMRAHQRKGAVLWPVVVAMGPGIILGSLAGPQIA
                     SALPTRVFAALFGAFTWFSAARMLVAREPKAGRELPGKTRLFGVGAGIGIVSSLVGAG
                     GGFLSIPFLGRHNVKIHRAVATSAALGLPIAAAGTLGFIIAGLRQSGLPRWSIGYVYL
                     PAMSVIVVASMLVAPVGAALAHRWPAVKLRRAFAALLVTLGAYMFWKSIRG"
     gene            complement(2952..3617)
                     /locus_tag="VFO53_06670"
     CDS             complement(2952..3617)
                     /locus_tag="VFO53_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HET8875995.1"
                     /translation="MRNLRKISALALFAAFAGAACAQTAVIQATVADRQGRPVADAVV
                     VAVPADASVRLPAKPHEGVVDQIDKQFVPKVQVVDVGTAVMFPNNDHVRHQVYSFSPA
                     KRFELPLYAGVPAKPILFEKPGVVVLGCNIHDWMIGYIYVSESPYFAKTRADGTAALT
                     NLPPRAYIVRVWHPQMKVAEETTRKTIEVMPGTRATVAWTLNLEPETGIRRAPAPGQG
                     RGY"
     gene            complement(3630..4535)
                     /locus_tag="VFO53_06675"
     CDS             complement(3630..4535)
                     /locus_tag="VFO53_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8875996.1"
                     /translation="MGSLKRLAHCAPARNAFSAVALFAALGTPARAEWLFDVAAGATY
                     DSNLTRAANAPDVRPGVAATFDVGAGEFIAMTGNDGVTLTANVRGESYARYHGLDVAG
                     IGATAAYRHKFGLGWGAPWAALTVTGAYDQYRSELRDGAHYDVRAEVGRRFTKRADVV
                     LGIACDRRYAPDADSEVPGIPGNPFALAGRRAYVQAGYALGEQWLFSVDGAIRRGDVE
                     STSQQGAAVFAASSAIAEDPAFRDPRLYAYRLRGTTATLGASLNFAWNEHSSLDLRYR
                     YAATRSPQSLDYRSSNVELLFAYRL"
BASE COUNT          749 a         1527 c         1550 g          794 t
ORIGIN      
        1 gctgcaccgg ccgggaccgc gatttgccga cggcatagaa gcgctgtgcc gcacgctcgc
       61 gaacgcgagg cgcgcgtacg cgtcgaacgc gtggagggag ctttcgaaat gagcaaggca
      121 ttcacgaacg aagaagcggc cgaaagcgac gacggcgaaa tcgtcgagcc gtcgccgctg
      181 cctgcgggcg cacgcaatta catgacgccg ggcggattcg cgcgcatgcg ccgcgagctc
      241 gatacgctgg tggcgaagga gcgacccgag ctggtcgcga cggtggcgtg ggccgccggc
      301 aacggcgatc gatccgaaaa cggcgattac atctacggca agaagcggct tcgagaaatc
      361 gaccggcgca tccgcttcct cgtgcgccgg ctcgacgccg cggaggtggt cgatcccgcg
      421 atgccgcgcg acgacgacgc ggcgtcgcgc gtctatttcg gcgcgaccgt gacgatagcg
      481 gacgccagcg gtggcgagcg cacggtgagc atcgtcggca tcgacgagat cgacactgcg
      541 cgcggctaca tcagctggat atcgccgatg gcgcgcgcgc tgctcagggc gagcgaaggc
      601 gatacggtca gcgtgcatac gccgggtggc atcgaagagc tcgacgtcgt cggcgttcgc
      661 tacgtggcac ttgccaccga tccctgatgc gcggtggttg actcgccgat cgtgcgcctt
      721 ctatagtgcc gcaaccgtgg acgccgatct cgccgcactc gaagaacgtc tcgacacgct
      781 gatcgcgtat gcgcgcgagc tgcgcgcggc caacgagctg ttgcgcagcg agctgcttgc
      841 atcgcaggag cgcaaccgcg acctgaccgc acgcgtgacg caggccaccg agcgcctcga
      901 tgcactgatc gcgcggatgc cggtggaatg atgcgcaagg aggccggcga atgacgcaga
      961 acgcagcacg caccgtgacg ctcgacgtaa cgatcctcgg gcgcgatttc aaggtcgcgt
     1021 gcaaggaaga cgagcgcgac gaactgctgg aggcggtcgc gctgctcgat cggcgcatgc
     1081 gcgagattcg tgacgccggc aaggtaagcg gcaccgatcg catcgcggtc atggccgggc
     1141 tcaacatcgc gcatgagctg ttgcgcgagc gccgcgcgtc ggcgatcgcc gtcgacgctc
     1201 ggcccgcaac gggcggcgaa tcctttgatg ccgaggccgc gcggcgtaga atccaggcaa
     1261 tgcaggcgac gatcgacgag acactcgccg gacaggaaaa tctgttctaa tcggttgaac
     1321 gcggttcgcg gcacgcgaac tgcaacatcc actccatcaa tcccctgcgg tgttcgatcc
     1381 ggccttacgt tctttgaacc aatgctctcg tagcgaggtc gccgagccag cggaccggtg
     1441 tgcgttccca cgcggaagcg cctgatggtc ccggaaggcg accgatcttg aaccccggtt
     1501 caggatgacc ggtcggaacg gcacaggcgg ggggcctatt cttccgggat caccgcgctg
     1561 atcccgtcct tgcccgatcg gcctcgcgcc ggcatgcgaa actgcgtttc cctgcgctga
     1621 agagacgccg tgcgacgcta ctggctgatg aaatccgagc ccgacgagtt ctcgatcgac
     1681 gacctcgtcg cggcgccgtc gcagaccacg ccgtgggtcg gcgtgcgcaa ctaccaggcg
     1741 cgcaacttca tgcgcgacgg catgcgtgtc ggcgatcgcg cgttcttcta tcactcgagt
     1801 tgtccggaac ccggcgtcgc gggcgtcgtc gagatcgcgc ggcgtgccta tcccgaccag
     1861 acgcagttcg atccgaaaag cgcgtactac gatccgaagt ctccacgcga cgcgccgcgc
     1921 tgggtgcacg tcgacgtgaa gctcgtgcgc aagacgcggc tcgtcacgct gccgatgctg
     1981 cgcgcgacgc ccggactcga ggacatggtc gtcctgcgcc gcggcaatcg gctgtcgatc
     2041 acgccggtga cgccggccga gtggaagatc gtcgagaagc tggcgaagtg accgactgga
     2101 tgctgctgcc ggtgttgctc gcgttcggcg ggctggtcgg ctttctcgcc ggattgctcg
     2161 gtatcggcgg cggcatgacg ctggtcccgc tgctgacgat cctcttcacc gcgcgcgact
     2221 ttcctgccga tcacatcatc cacatggcgg tcgcgaccgc gacggccacg atggtgttta
     2281 ccgcgatgtc atcgatgcgt gcgcatcagc gcaaaggcgc cgtgctgtgg cccgtcgtgg
     2341 tcgcgatggg tcccggcatc atcctgggat cgcttgccgg cccgcagatc gcgagcgcgc
     2401 tgcccacgcg cgtcttcgcc gcgctgtttg gcgcgttcac ctggttttcg gcggccagaa
     2461 tgctggtcgc gcgcgaaccc aaggccgggc gcgagctgcc gggcaaaacg cggctcttcg
     2521 gcgtgggtgc gggcatcggc atcgtttcga gcctggtcgg ggcaggcggc ggctttctgt
     2581 ccattccctt cctcggccgg cacaacgtga agattcaccg cgccgtcgcc acgtcggccg
     2641 cactgggcct tcccatcgcg gcggcgggca cgctcggctt catcattgcg ggtcttcgcc
     2701 agtccgggct cccgcgatgg tccatcgggt acgtctatct gccggcgatg tcggtgatcg
     2761 tcgttgcgag catgctcgtg gcacccgtcg gtgcggcgct tgcgcaccgg tggccggccg
     2821 tcaagctgcg ccgtgcgttc gcggcgctgc tcgtgaccct cggtgcgtac atgttctgga
     2881 agtcgattcg cgggtgatcg cggcgatcgg cggaccatcg tcggccgcga cccatgccgt
     2941 tgcatcgccc ttcagtatcc cctgccttgt cccggagcgg gcgcgcgccg gatccccgtt
     3001 tcgggctcga gatttaacgt ccatgcgacg gtggcgcgcg ttccgggcat gacctcgatc
     3061 gtcttgcgcg tcgtctcctc cgcgaccttc atctgcggat gccagacgcg cacgatgtag
     3121 gcgcgcggcg gcagattcgt gagcgcggcc gtcccgtcgg cgcgtgtctt cgcgaagtac
     3181 ggtgattccg acacgtagat gtagccgatc atccagtcgt gaatgttgca gccgagcacg
     3241 acgacgcctg gcttttcgaa caggatgggc tttgccggca cgccggcgta cagcggcaat
     3301 tcgaagcgct tggccggcga gaacgaatac acctggtggc gcacatggtc gttgttcgga
     3361 aacatgaccg ccgtgcccac gtccacgacc tgcaccttcg ggacgaattg cttgtcgatc
     3421 tggtcgacga caccctcgtg cggcttcgcc gggaggcgca cgctcgcatc cgcgggcacg
     3481 gcgacgacaa cggcgtcggc gaccggcctt ccctgccggt cggcgaccgt cgcctggatc
     3541 acagccgtct gcgcacacgc agcgccggcg aacgcggcga agagcgcgag tgcagaaatt
     3601 ttccggaggt ttcgcatcac gttcttccgt cacagccgat acgcaaacag gagttcgaca
     3661 ttgctgctcc gatagtcgag agactgcggt gagcgcgtcg ccgcgtagcg gtagcgcagg
     3721 tcgagcgacg agtgctcgtt ccatgcgaaa ttcaagcttg cgcccagcgt tgccgtcgtt
     3781 ccgcgcaggc gatacgcata caggcgcggg tcgcggaaag ccggatcctc tgcgatcgcg
     3841 ctcgatgcgg cgaaaacagc ggctccctgc tgcgacgtcg actcgacgtc accgcgacgg
     3901 attgcgccgt cgacgctgaa cagccactgc tcgccgagcg cgtagcccgc ctggacatag
     3961 gcgcgtcgcc cggcaagtgc aaacgggttg cccggaatgc cgggcacctc cgaatccgcg
     4021 tcgggcgcgt aacgccggtc gcaggcgatc ccgagcacca cgtccgcgcg cttcgtgaat
     4081 cgtcgtccga cctctgcgcg aacatcgtag tgcgcgccgt cgcgaagctc gcttctgtat
     4141 tggtcgtacg cacccgtcac cgtcaacgct gcccatggcg cgccccagcc gagtccgaac
     4201 ttgtgccggt acgcggcggt ggcaccgatg ccggcaacat cgagtccgtg gtaacgggca
     4261 tagctttctc cgcgcacatt cgcggtcagc gtcacgccgt cgttgcccgt catcgcaatg
     4321 aattcaccgg cgccgacatc gaaggtcgcc gcgacgcctg gccgcacatc gggcgcgttt
     4381 gcagcgcggg tgagattgct gtcgtacgtt gcgccggctg cgacgtcgaa cagccactcc
     4441 gctcgcgccg gcgtgccgag tgccgcgaac agggcgaccg ctgaaaaggc gttccgtgcc
     4501 ggtgcgcaat gcgcaagtcg cttcagcgag cccatggtct tgcggacgcg acatcccggg
     4561 ggcaggaatc ttgctttgtc atgtcgcgtg catcgcaaga tgcgtaccca cagcctgatc
//
LOCUS       DASSVX010000152         3097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1339000, whole genome shotgun sequence.
ACCESSION   DASSVX010000152 DASSVX010000000
VERSION     DASSVX010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339000"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..528)
                     /locus_tag="VFO53_06680"
     CDS             complement(223..528)
                     /locus_tag="VFO53_06680"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HET8875997.1"
                     /translation="MIRIYVARQRFDAYLVADRLNQAGIRAHVFNEHASSIVGDVPPD
                     VAQPQVWLADDADAPRAQALLRTMERDAARTPLFCRKCGEQSPGSFELCWNCGTAIA"
     gene            complement(525..1313)
                     /gene="hutG"
                     /locus_tag="VFO53_06685"
     CDS             complement(525..1313)
                     /gene="hutG"
                     /locus_tag="VFO53_06685"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HET8875998.1"
                     /translation="MLLWQLTQGHSPLIVNVPHAGRHVPDALALRMDPRALALHDTDW
                     HVDALYRFAPATGATFVVATHSRYVVDLNRDPSGAPLYPGADNTELCPTRTFANEPVY
                     RDGEAPAQSEIAAREVTYFAPYHALLAQQIERVRSRHGYALLLDGHSIAAEVPRFFAG
                     RLPDLNLGTVDGRSCGASVQTLATRVLAQANGFTHVVNGRFKGGYITRHFGAPGRGVH
                     ALQLEMAQACYMDESQPAALDAARAAPLVALLERLVIALSEWRA"
     gene            complement(1347..2720)
                     /locus_tag="VFO53_06690"
     CDS             complement(1347..2720)
                     /locus_tag="VFO53_06690"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HET8875999.1"
                     /translation="MKTLIAPAALLDDGWKSDVAIGVGDDGRITTIEPGARTVADERL
                     KGPVIPAMPNVHSHAFQRAIAGRTGTGSGERNDSFWTWRQAMYAAVERLDADAFEAIA
                     AQAYVEMAKSGYASVAEFHYVHHDPHGKPYADPAELAWRIVGAAETAEIALTLLPVFY
                     AHGDFGGAASTPAQRRFVHSVYTFERLCDALARGATTRHYVLGVAPHSLRAVTPQELS
                     KIVRFAAPDAPIHIHAAEQTREVDDCCKWSGLRPVEWLLANASVDARWCIVHATHMTD
                     REVAGLAASGAVAGLAPGTEADLGDGIFPGEAYLHAGGRFGIGSDSNTMIDPFAELRQ
                     LEWSQRLRLRRRNVMAGSPDRPVGESLWRTAAQGGAHALGLGAGRLAPGMRADLIVLD
                     ADDPALVEQPVANVLDAAIFGPCRRPVRDVMSGGRWIVRDGHHAREQDVMRRFRAALA
                     RIRPGGA"
     gene            2872..>3097
                     /locus_tag="VFO53_06695"
     CDS             2872..>3097
                     /locus_tag="VFO53_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5329 family protein"
                     /protein_id="HET8876000.1"
                     /translation="MTVHRIRRPLAATVATLAFAITHAAAAATGAPATDEIDHLLRYV
                     AASSCTFVRNGTEYPADKASEHLASKYRFVG"
BASE COUNT          481 a         1067 c         1077 g          472 t
ORIGIN      
        1 gttcatgacg agctccctct tcggttgtcg aatttttggc gcggcacctg aaggcacgca
       61 gcgtcgttcg tcgcatcgga cgacgcacga acgctggatc aacgtctcac tggatcgatg
      121 cgatgaccac ggcacaccgg caggtgtaac ggattatgac agcggcatgg ccccgcggga
      181 accgccgccg gtcgccgcga ttgccggcgc ctttgatccg tcctacgcga tggcggtgcc
      241 gcagttccag cagagctcga agctgcccgg cgactgctcg ccgcacttgc ggcagaaaag
      301 cggcgtgcgc gcggcgtcgc gctccatcgt gcgcagcaac gcctgcgcgc gcggtgcgtc
      361 ggcgtcgtcg gccagccaca cctgcggctg agcaacgtcg ggcggaacgt caccgacgat
      421 gctcgacgcg tgctcgttga agacgtgcgc gcgtattcca gcctggttca gccgatcggc
      481 gacgagatac gcgtcgaagc gctggcgcgc aacgtagatg cgaatcacgc gcgccactcc
      541 gacagcgcga tcacgagccg ctcgagcagc gcaacgagcg gcgccgcgcg cgccgcgtcg
      601 agcgcggcgg gttgcgattc gtccatatag caagcttgcg ccatttcgag ctggagcgca
      661 tgcacgccgc ggccgggcgc gccgaagtgg cgcgtgatgt agccgccctt gaagcggccg
      721 ttcacgacgt gcgtgaagcc gtttgcctgc gcgagcacgc gagtcgcgag tgtctgaacc
      781 gatgcgccgc agctgcgtcc atcgaccgtt ccgagattga gatcgggcaa gcgtccggcg
      841 aaaaagcgcg gcacttccgc ggcgatcgaa tggccgtcga gcagcagcgc gtagccgtgg
      901 cggctgcgca cccgctcgat ctgctgcgcg agcagcgcgt ggtacggcgc gaagtacgtt
      961 acctcgcgcg cggcgatctc cgattgcgcc ggcgcctcgc catcgcgata gaccggctcg
     1021 ttcgcgaacg tgcgcgtggg acagagctcg gtgttgtcgg cgccggggta cagcggcgcg
     1081 ccggagggat cgcgattgag atcgacgacg tagcgcgaat gcgtcgcgac gacgaaggtg
     1141 gcgccggtgg caggcgcgaa ccggtacagc gcgtccacgt gccagtcggt atcgtgcagc
     1201 gcgagcgcgc gcgggtccat gcgcaatgcg agtgcatcgg ggacgtggcg gccggcgtgc
     1261 ggcacgttca cgatcagagg tgaatgtccc tgagtcagtt gccacagcag catgcgtcga
     1321 tcccgtgcgg gcgagggcgt cgcgcgtcag gccccgcccg gccggatgcg tgcgagcgcc
     1381 gcgcgaaagc gccgcatcac gtcctgctcg cgcgcgtgat gaccgtcgcg cacgatccag
     1441 cgcccgccgc tcatcacgtc gcgcacgggg cggcggcacg gtccgaagat cgccgcgtcg
     1501 agcacgttgg cgacaggctg ctcgacgagc gctggatcgt cggcgtcgag cacgatcagg
     1561 tcggcacgca tgccgggggc gaggcgccct gcaccaagcc cgagcgcgtg cgcgccgccc
     1621 tgcgccgccg tgcgccacag cgattcgccg accggcctgt ccggggagcc ggccatcacg
     1681 ttgcgccggc gcagacgcag ccgctgcgac cattcgagct gccgcagttc ggcgaacgga
     1741 tcgatcatcg tattcgaatc gctgccgatg ccgaagcgtc cgcccgcgtg caggtacgct
     1801 tcgccgggga agatgccgtc gccaagatcg gcctcggtgc ccggtgcgag gcccgcgact
     1861 gcgccgctgg cggcgaggcc cgcgacttcg cggtcggtca tatgcgtcgc atgcacgatg
     1921 caccagcgcg cgtcgacgct cgcattcgcg agcagccact ccaccggccg caggccgctc
     1981 catttgcagc aatcatcgac ttcgcgcgtc tgctcggcgg cgtggatgtg gatcggggca
     2041 tcgggtgctg cgaagcgcac gatcttcgaa agctcctgcg gcgtcacggc gcgcaggctg
     2101 tgcggcgcga cgccgagcac gtaatggcgc gtcgttgccc cgcgcgcgag cgcgtcgcaa
     2161 agccgctcga acgtgtacac gctgtgcacg aagcgccgct gcgcgggcgt gctcgctgcg
     2221 ccgccgaaat cgccgtgcgc atagaacacg ggcagcagcg tcaatgcgat ttccgcggtt
     2281 tccgccgcgc ccacgatgcg ccatgcgagc tcggcaggat cggcgtacgg cttgccgtgc
     2341 ggatcgtgat gcacgtagtg aaactcggcc accgacgcgt agccggactt cgccatctcg
     2401 acgtaggctt gcgccgcaat ggcctcgaag gcatcggcat cgagccgctc gaccgccgcg
     2461 tacatcgcct ggcgccaggt ccagaacgaa tcgttgcgct cgcccgagcc cgtgccggtg
     2521 cgcccggcga tggcgcgctg gaacgcgtgc gagtgcacgt tgggcatcgc ggggatgacg
     2581 ggccccttca gccgctcgtc cgcaaccgtg cgggcacccg gctcgatcgt cgtgatgcga
     2641 ccgtcgtcgc cgacgccgat cgcgacgtcg ctcttccagc cgtcgtcgag cagtgcggcg
     2701 ggtgcgatca gcgtcttcat ccgagccgtg ggtcgttcat cgttgatcag gcgctaaatc
     2761 ggggcgcgac ggcatgcgcc gattgtaacc gcgcgaagcg acgccacatg ggaaccgcgc
     2821 ggcgacgatc ctgtcggaca cgcgcctacc ctttttgccc gagacacacc gatgaccgtg
     2881 catcgcatcc gccgtcctct cgctgcgacc gtcgccacgc tcgccttcgc gattacacac
     2941 gccgccgcgg cagccaccgg cgcaccggcc acggacgaga ttgaccacct gctgcgctat
     3001 gtggccgcgt cgtcctgcac gttcgtgcgc aacggcaccg agtatcccgc ggacaaggcg
     3061 agcgagcatc tcgcgagcaa gtaccgtttc gtcggcg
//
LOCUS       DASSVX010000153         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_39225, whole genome shotgun sequence.
ACCESSION   DASSVX010000153 DASSVX010000000
VERSION     DASSVX010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39225"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFO53_06700"
     CDS             complement(<1..417)
                     /locus_tag="VFO53_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5118 domain-containing protein"
                     /protein_id="HET8876001.1"
                     /translation="MSHPSLSRVGVALGILAPLVMSGCASLARPDAVRPGETAAVAPS
                     ALRDDAPGNAGHADAPGAPRPGGPATPYARPFVEVVKDAKETAGLFNIWQKDDKVFLE
                     LSPDQFDKPFFFKSAINQGIGEARIFGGSMNYPFGVA"
     gene            complement(486..1811)
                     /gene="miaB"
                     /locus_tag="VFO53_06705"
     CDS             complement(486..1811)
                     /gene="miaB"
                     /locus_tag="VFO53_06705"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET8876002.1"
                     /translation="MARKLFIRTFGCQMNEYDSDRMADVLAREGLALTDRPDDADVIL
                     FNTCSVREKAQERVFHDLGRVRDLKAHRPDLLIGVGGCVASQEGGAIVRRAPYVDLVF
                     GPQTLHRLPAMMRARRESGEPQIDVSFPEIEKFDHLPPPRVDGASAFVSIMEGCSKYC
                     SFCVVPYTRGEEVSRPFEDVLTEIADLALQGVKEVTLLGQNVNAYRSNTDTGEPCDLA
                     RLLEYAADMPGIERLRYTTSHPKEMTQRLIDAYASNGKLVSHLHLPVQSGSDRVLAAM
                     KRGYTALEYKSIVRKLRARRPDLSLTSDFIVGFPGETDDDFDRTLRLVDDLAFDGAFS
                     FAYSARPGTPAADLPDQVPDDVKQARLARLQTLLDSQYKANSERMIGTRQRVLVTGTA
                     ARNPRELAARTDNNRVVNFAGDQAAIDHYVDVAITAAMPHSLRGELTLA"
     regulatory      1850..2015
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          361 a          709 c          733 g          359 t
ORIGIN      
        1 cgcgacgccg aacggatagt tcatgctgcc gccgaaaatg cgcgcttcac cgatgccctg
       61 gttgatcgcc gacttgaaga agaacggctt gtcgaactgg tctggcgaaa gctcgaggaa
      121 gaccttgtcg tccttctgcc agatgttgaa gaggcctgcc gtttccttcg catccttgac
      181 cacttcgacg aacggtcgcg cgtacggggt cgccggaccg ccgggacgcg gagcgccggg
      241 ggcatcggcg tgccccgcat tgcccggcgc atcgtcgcgc agcgccgacg gcgcgacggc
      301 cgccgtttca ccgggcctca cggcgtcggg gcgcgccagg ctcgcgcaac cggacatcac
      361 gagcggagcg aggatgccga gcgcgacgcc tacgcgggaa agggaaggat gcgacatgaa
      421 cgaggtatct cctgaatcga cgagggcctt gcgcgcgccg ccaatgcggc cgcgacgaag
      481 accgttcacg cgagcgtaag ttcgccgcgc agcgaatgcg gcatcgccgc ggtaatggcg
      541 acgtcgacgt aatggtcgat cgccgcctgg tcgcccgcga aattgacgac tcggttgttg
      601 tcggtgcgcg cggccagttc gcgcggattc ctggcggccg tcccggtgac gagcacgcgc
      661 tgccgcgtgc cgatcatccg ttcgctattg gccttgtact ggctgtcgag cagcgtttgc
      721 agccgggcga gccgcgcctg cttgacgtcg tccggcacct gatcgggcag gtcggcagca
      781 ggggtgcccg gacgcgcgct gtacgcgaag ctgaacgcgc cgtcgaacgc gaggtcgtcg
      841 acgaggcgaa gcgttctgtc gaagtcgtcg tccgtctcgc cggggaagcc gacgatgaag
      901 tcggatgtga gcgacaggtc cgggcgccgc gcgcgaagct tgcgcacgat cgatttgtat
      961 tcgagtgccg tgtagccgcg cttcatcgcg gcgagcacac gatcggaacc cgactgcacg
     1021 ggcaggtgca gatgcgacac gagcttgccg ttcgatgcgt acgcgtcgat cagccgctgc
     1081 gtcatctcct tcggatgcga cgtcgtgtaa cggagccgct cgatgccggg catgtcggcc
     1141 gcgtattcga gcagccgtgc aagatcgcag ggctcaccgg tgtccgtgtt gctccggtac
     1201 gcattgacgt tctgtccgag cagcgtcact tccttcacgc cctgcagcgc aagatccgcg
     1261 atctcggtca gcacgtcctc gaagggccgc gagacctcct cgccgcgcgt ataaggcacg
     1321 acgcagaacg agcagtactt gctgcagcct tccatgatcg agacgaacgc cgacgcaccg
     1381 tcgacacgcg gtggcggcag gtgatcgaac ttctcgatct ccggaaacga gacgtcgatt
     1441 tgcggctcgc cgctctcgcg gcgcgcgcgc atcatcgccg gcaaccggtg cagcgtctgc
     1501 gggccgaaca cgagatcgac gtacggcgcg cggcgcacga tggcgccgcc ttcctgcgac
     1561 gcgacgcaac cgccgacgcc gatcagcaga tcgggacggt gcgctttgag gtcacgcacg
     1621 cgcccgagat cgtggaacac gcgctcctgc gccttctcgc gcacggagca cgtgttgaac
     1681 aggatcacgt ccgcgtcgtc gggacggtcg gtcagcgcaa gtccttcgcg tgcgagcacg
     1741 tcggccatgc ggtccgagtc gtactcgttc atctgacagc cgaacgtgcg aatgaaaagc
     1801 ttgcgcgcca cggtcattgc gggaaagggt cgcgattata gcaaaccgtc gtacaatcct
     1861 cgcagcaggt gtccgccggg cggcgtggtc gagtcgaccg gcgggtgaaa cgggaatgcg
     1921 gtgcgcgcgc gatttcgcgc gcaatgccgc agctgccccc gcaacggtga gccgaaaggc
     1981 gagcccggag accggcctgc tgacccctta tcccaaggta gggccgtgcg gggtactcac
     2041 ggcatgacgc ggatggctcc gaattcccgt tctcctctgc aacgtcgcgg gcgcgcgctc
     2101 ggatggctcg cgcccatcgt cctcgttccc gccattgccg gtgcacagtc cccgccgacg
     2161 gc
//
LOCUS       DASSVX010000154        11662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_689088, whole genome shotgun sequence.
ACCESSION   DASSVX010000154 DASSVX010000000
VERSION     DASSVX010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 11662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11662
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689088"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFO53_06710"
     CDS             complement(<1..303)
                     /locus_tag="VFO53_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876003.1"
                     /translation="MPLGYGALHQETMPRHGSPLVEPILIDLEERTFRFFWDTANPKN
                     GLIPDRYPTPSFASIAAVGFGLTSYPIGVERGYITRNEAVKRVLTTLRFLYNAPQGT"
     gene            complement(494..1411)
                     /locus_tag="VFO53_06715"
     CDS             complement(494..1411)
                     /locus_tag="VFO53_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876004.1"
                     /translation="MTRLGLVALALMLAAGARAQAPAALGNIDVESDTDGFHASRVRI
                     GALYPYGSYVDHLGAAVQSTHYSQGNWSRDASGIVGLWRDQDARTLTGVNAEAGVVRV
                     AGHTRAVGDATWGLRPAANTGVELIAAGDLVATRKAIERGIAYGFVAASVEQTFAERF
                     TAIALAGYQPFTDGNDRVHWRGRLIWQAVPDYGVNVQLRYRGYESGRSDVGGAYFNPD
                     RYRQWQGALGVRRRVGSWVWTASAGAGRETINSNDTHPVRVAELRGEGAVTDRVRLAL
                     YALYSRSTGYVDAPDYAYRQVGVTLIYPF"
     gene            complement(1408..2898)
                     /locus_tag="VFO53_06720"
     CDS             complement(1408..2898)
                     /locus_tag="VFO53_06720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8876005.1"
                     /translation="MRNDAIHPASADNSPLGREILDPQPAPGSAGEIALARAIIRPLA
                     DTAYDNARTVASTERATSNAPFYVGVRGKFALALLVSGGWFALTVLIALPWIAELSLR
                     IGAPLALLAVGGIALVPGFMNAFLIASLMLDRRPPRSKPSRYPPVSILIAAYNEEASI
                     ADTLRSIANQDYPGAFEVFVIDDGSHDRTAAIVDACDNDWLHLLRQPHNAGKSAALNR
                     GLAEARFDLVVTLDADSFLYRDALRNLVERYLSDPPNTRAVAGTMLVRNSRRNWVTKA
                     QEWDYFHGIAAIKRVQSLYQGTLVAQGAYSLYERAALREIGGWVDCVGEDIVLTWAML
                     RKGWRVGHAEDACCFTNAPESLHQFVRQRQRWARGMMEAFRQFPDILTKPRLSTLFVW
                     WNLLFPWLDLAYTLFFIPGVLLALFGIYWIAGPLTLALLPMALGINYAMYRVGSQMFA
                     SNGLRVRRNLSGFLTYAFVYSLILQPACVAGYFSEVLGLRKTWGTK"
     gene            3020..3619
                     /locus_tag="VFO53_06725"
     CDS             3020..3619
                     /locus_tag="VFO53_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET8876006.1"
                     /translation="MTTPPAPPLDADPFAIELDTHRRYLLRVARLQLRDGDLAEDVVQ
                     ETFLAAVAGRASFSGRSSVRTWLTGILKHKIIDAIRQKQRHPTLAAAFDETLDVDALD
                     GMFTEEGAWKTPPADWGNPETALAQSEFMDVLARCLDKLPPATARVFTMREILELESD
                     EIRKELSITANNLWVILHRARVSLRRCLEQNWFASPARR"
     gene            3633..3824
                     /locus_tag="VFO53_06730"
     CDS             3633..3824
                     /locus_tag="VFO53_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876007.1"
                     /translation="MLHRLTHVVSCREASRLLSRREERPLATVERVKLALHLRLCTAC
                     TRFARQLAFIRRALARYRS"
     gene            3827..4027
                     /gene="thiS"
                     /locus_tag="VFO53_06735"
     CDS             3827..4027
                     /gene="thiS"
                     /locus_tag="VFO53_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HET8876008.1"
                     /translation="MLTLTLNGVATGVECATLAELVASLGLEGKRIAVERNGEIVPRS
                     RYADTPLVRGDRLEIVGAVGGG"
     gene            4051..4860
                     /locus_tag="VFO53_06740"
     CDS             4051..4860
                     /locus_tag="VFO53_06740"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET8876009.1"
                     /translation="MNAPDNPAALADPLRIAGRAYRSRLLVGTGKYRDFTETRDALVA
                     SGAEIVTVAIRRTNIGQTPGEASLADALPPSEFTYLPNSAGCYTADDAVRTLRLGREL
                     LDGHALCKLEVLGDASSLYPNVRETLVAAERLVADGFQVMVYTSDDPIVARELEAIGC
                     VAIMPLASLIGSGMGIVNPWNLRLILDEARVPVIVDAGVGTASDATIAMELGCAGVLM
                     NTAIAKAREPVRMAHAMKLAVESGREAFLAGRMPRKHYAGTPSSPTTGLIA"
     gene            4875..5525
                     /locus_tag="VFO53_06745"
     CDS             4875..5525
                     /locus_tag="VFO53_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876010.1"
                     /translation="MRRHVRWAFGALLLPAFAHAAPIPRGELMTLCAEADDQAHCGRL
                     VEARQLRSLSRIAERNGDELRVALAPFGLSVFHDTVNVKGATTYAVWDYLERVDTLVL
                     FTTRGERTGFMLVQRQGGGEYRVPAEPVLAPDDRRFATADFCANECDNEVAIWHIGSD
                     GIRKEAVWTPPAAWSDVSVAWRNANSIALDYALDDGQRRTIERRLDDPSWKKARAR"
     gene            5684..6322
                     /gene="trmB"
                     /locus_tag="VFO53_06750"
     CDS             5684..6322
                     /gene="trmB"
                     /locus_tag="VFO53_06750"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET8876011.1"
                     /translation="MSPAQQRACDELYPRYGVNPSAPLDLAAIFGRRAPVVLEIGFGM
                     GETTAAIAATQPGVDFLGVEMHSPGVGALLRRIESMQLANVRVLRHDAVDVVARMIPA
                     ASLAGIHVYFPDPWPKKRHHKRRLLKPPFVHALAAALAPGGYLHVATDWAAYADEILA
                     ALAAEPLLANTAAGFAPRPAWRPLTKFEQRGLARGHAVFDVLFTSVRAQAAR"
     gene            complement(6291..8843)
                     /locus_tag="VFO53_06755"
     CDS             complement(6291..8843)
                     /locus_tag="VFO53_06755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8876012.1"
                     /translation="MDRERYVERYMAPLASSKRAVTGEVGEFDPRHFEPPDVGREAAP
                     LTLSQLFVAGALCGHAEARAELGRRSDAADRDLATLDEARRRRDAAGDGIALGLIEAE
                     RQLAALGQRTRALEESAQASRRRVGEIESSTTWRMTAPLRSGVHRVKIVVAALQTQWR
                     TARQLPRHAGTALTILRNDGAAALARRVVRKLRARPRFVPPIRPLARLEPRIAPLALR
                     TSKAPRTSIVIPAYGEPLLTFSCLASIARHTSGEFEVIVVDDASEQPLAECLAQVSGV
                     RFERNAANLGFVGTCNRGAAIARGDTLVFLNNDTLVTAGWLDALMRVLDEDPDTGLVG
                     ARLVYADGRLQEAGGIAWRDGSAWNFGRGDDPDRPEYNYLREVDYCSGACLAIPRALF
                     AQLNGFDTRYAPAYYEDVDLAFAVRAAGRKVVYQPAATIVHFEGQTSGTDVTQGVKRH
                     QAVNQSAFAAKWASALGAHRPNGMHVELERDRFATKRMLVIDARMLTPDQDAGSLRMQ
                     AMLEIATALRCKVTFVADNLEHGAPYVDALTQRGVEVLFHPYVRSISELLMRRAQEFD
                     VIMLSRHYIAARHIDTVRKAAPRALIVFDTVDLHFLREERLAKLEGGRLAAHSARSRR
                     ADELALIAKADVTLVVSDAEQAVLKELAPASRVMLLSTIHEPVARVPGWAARRGIVFV
                     GGFEHPPNVDAMRWYAKEVMPHVRRLLPGVSTYVIGSRVTASIEALAADDFVVLGYVP
                     DIAPYLEACRVSISPLRYGAGVKGKINTAMSHGLPVVATTVSVEGMHLADGVDVLVAD
                     EPEAFAAAIARVHEDGALWERLSAGGRDNVARHFSRDIAREALTALLALAPK"
     gene            complement(8843..10711)
                     /locus_tag="VFO53_06760"
     CDS             complement(8843..10711)
                     /locus_tag="VFO53_06760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8876013.1"
                     /translation="MSGAATRPARGATVVDIVLPAEGDIDALRRSVESILASANVTPF
                     HVVVVADAGASADAAAALRDIAANARVTLLPAERDASGTQTLERVLELHRDRDVVLMA
                     PGAEVHGDWLDRLASHVRGDAIGAVGAFSNVANAAAYPRPATANALPDGETAASLDAL
                     FARVNAGRSVGVDAVEGPCRYITRAAITATGGAPIGMGGDDAGVMRDWTGRAASAGFR
                     ALIAGDVFVAQNPSTLSDTLPVASSVQTVPLARRIDIARLAASPRPAIVFVSHAWGGG
                     IRRYMDDLAALVRERADVLYLEPAGGDTVRLHWPRDGEAFAAWFRLPDQMTALAGMLR
                     DIGAARLHFHHVHGMPQSILDLPRAAGIAYDCTLHDYFAICPQYHLADEHGRYCGEPD
                     RAGCASCLVHRRAQWPLDIDAWRARFAEFFSSAARVIAPSHDVKARMHRYFPALPIVV
                     WPHPEPEATPLPRIVRVVTLGGLSPEKGLHIVAECAKEAQARGLPLAFRVLGATAEPI
                     AQSPDVPLTLHGSYVEARLPALLAAEHADVLFFPAQVPETYSYTLSVALATDTPIVAS
                     AIGAFPERLAGRALVRMLPFDAPPSVWNDALIGVAREAVGYPAGASVAATPLHAES"
     gene            complement(10734..11591)
                     /locus_tag="VFO53_06765"
     CDS             complement(10734..11591)
                     /locus_tag="VFO53_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET8876014.1"
                     /translation="MKLITWNVQWCRGVDGAVDPARIVAEARRLADFDVLCLQEIADN
                     YPDPRLAGSDGEDQFARLAQLLPGFTAVPGIAVDQPGDDGRRRRFGNMILSRLPVRQV
                     YRHLLPYPCDPGVPGMPRIAVEAVLGEDGHDVRVITTHLEYYSAKQRMAQVEALRAIY
                     AEGHAYARGAQVTTSDGGPFQTFIRPRETVITGDCNFEPDSPEHRRMLAPFADGTPAL
                     VDAWSALQPGRAHPPTFKIYEKKRPDEPELHCDFIFIGEELRARMRDVRVDVDTQAAD
                     HQPVILTLA"
BASE COUNT         1894 a         4048 c         3976 g         1744 t
ORIGIN      
        1 cgtgccctgc ggcgcgttgt agaggaagcg caacgtcgtg agcacgcgct tcaccgcctc
       61 gttgcgggtg atgtagccgc gctcgacgcc gattgggtac gacgtgaggc cgaagccgac
      121 cgcggcgatg ctcgcgaacg acggcgtcgg ataccgatcg ggaatgagcc cgttcttcgg
      181 attggcggtg tcccagaaga agcggaacgt gcgctcctcg aggtctatca ggatcggctc
      241 gacgagcggg ctgccgtgac gcggcatcgt ctcctggtgc aacgcgccgt aaccgagcgg
      301 catgttgctg ccataggcga cggcgcgatc gtcgacagat gcggcggcca gcatgccgag
      361 cgccgcgatg aacagtgcga cgacgatgaa acgccgcctg gtcgaatggc tagtcatgtc
      421 aatgtcattt ccgaaccggc gaagaatacc gtaaatgtcg ccggcgggcg acacccaaga
      481 attactgcac cgttcaaaac ggatagatca gcgtcacccc cacctgccgg tatgcataat
      541 ccggcgcgtc gacataaccg gtcgagcgac tgtagagcgc gtaaagcgcg agccgcaccc
      601 gatcggtcac ggccccttcg ccgcggagct cggccacgcg caccggatgc gtgtcgttgc
      661 tgttgatcgt ttcgcgtccc gcgcctgcgg aagcggtcca gacccagctt ccgacgcgcc
      721 ggcggacgcc gagcgcaccc tgccactgac ggtagcgatc ggggttgaag tacgcgccgc
      781 cgacgtcgct cctgccgctt tcgtacccgc gatagcgcaa ttggacattc acgccgtaat
      841 cgggcaccgc ttgccagatg aggcgcccgc gccagtgcac gcggtcgtta ccgtcggtga
      901 acggctggta accggcgagc gcgatcgccg tgaagcgctc ggcaaacgtc tgctcgacgc
      961 tcgccgcaac gaagccgtac gcgatgccac gctcgatcgc cttccgcgtc gccaccaggt
     1021 cgccggcggc gatcaactcg acgccggtgt tcgcggccgg ccgcaggccc catgtcgcat
     1081 cgcccacggc gcgcgtatgt ccggcaaccc gcacgacacc ggcttcggca ttcacgccgg
     1141 tcagcgtcct cgcgtcctgg tcgcgccaca atccgacgat gccgctcgca tcgcggctcc
     1201 agttgccttg cgaataatgc gtgctctgca cggcggcgcc caggtgatcg acgtacgagc
     1261 cgtacgggta cagcgccccg atgcgcacgc gcgacgcgtg gaagccgtcg gtgtcgcttt
     1321 cgacgtcgat gttgccgagg gccgccggcg cttgcgcgcg cgcgcccgcc gccagcatga
     1381 gcgccagcgc gaccaggccc aaacgcgtca cttggtgccc caggtcttgc gcagtcccag
     1441 cacctccgag aagtagccgg cgacgcacgc gggctgcagg atcaggctgt agacgaatgc
     1501 ataggtgagg aagccggaca ggttgcggcg cacgcgcaaa ccgttcgacg cgaacatctg
     1561 cgagccgacg cgatacatcg cgtagttgat gccgagcgcc atgggcagca gcgcgagcgt
     1621 cagcggcccc gcgatccagt aaatgccgaa cagcgcgagg agcacgccgg gaatgaaaaa
     1681 cagcgtgtac gcgagatcga gccacggaaa gagcaggttc caccagacga acagcgtcga
     1741 cagtcgcggc ttcgtcagga tgtcggggaa ctggcggaac gcttccatca ttccgcgcgc
     1801 ccagcgctga cgctggcgca cgaactgatg cagcgactcg ggcgcgttgg tgaagcagca
     1861 cgcatcctcg gcgtggccga cgcgccagcc cttgcgcagc atcgcccacg tgaggacgat
     1921 gtcctcgccg acgcaatcca cccagccgcc gatctcgcgc aacgccgcgc gctcgtacag
     1981 cgagtacgcg ccctgggcaa cgagcgttcc ctggtagagc gattgcacgc gcttgatcgc
     2041 ggcgatgccg tggaagtagt cccattcctg cgccttcgtc acccagttgc gccgcgaatt
     2101 gcgcaccagc atcgtgccgg caacggcacg cgtgttcggc gggtccgaaa ggtagcgttc
     2161 gaccagattg cgcagcgcgt cgcggtacag gaacgaatcg gcgtcgagcg tgacgacgag
     2221 atcgaaacgc gcctcggcaa ggccgcggtt caatgcggcc gacttgccgg cgttgtgcgg
     2281 ctggcgcagc agatgcagcc agtcgttgtc gcaggcgtcg acgatcgccg cggtgcgatc
     2341 gtgcgagccg tcgtcgatga cgaacacctc gaatgcgccg gggtagtcct gattcgcgat
     2401 cgagcgcagc gtgtcggcga tcgacgcttc ctcgttgtag gcggcgatca ggatcgacac
     2461 gggcggatag cggctcggct tgctgcgcgg cggccggcgg tcgagcatca ggctcgcgat
     2521 caggaacgcg ttcatgaacc ccggaacgag cgcaatgccg ccgacggcga gcagcgcgag
     2581 cggcgcgccg atgcgcaacg acagctccgc gatccacggc agcgcgatca gcacggtgag
     2641 cgcgaaccag ccgccgctca ccagcagcgc aagggcaaac ttgccgcgaa cgccgacata
     2701 aaacggcgcg ttcgatgtcg cgcgctcggt ggacgccacc gttcgcgcgt tgtcgtacgc
     2761 cgtatcggca agcgggcgga tgattgcgcg cgccagcgca atttcgccgg cgctgcccgg
     2821 cgcgggctgg ggatcgagaa tttcgcggcc gagcggcgag ttgtccgcgg aggccgggtg
     2881 tatcgcatcg tttcgcatga tgcgcgacca acggcaagtc gcgtgccatg ctcgactccg
     2941 tcgcgcgcga tggacgccac aaacgtcctc acatgttgca cgctacaacg cgcgcaggcc
     3001 tggttggcct aagatgacga tgacgacacc gcccgcgccg cccttggacg ccgacccttt
     3061 cgcgatcgag ctcgacacgc atcggcgcta cctgctgcgt gttgcacggc tgcagcttcg
     3121 cgacggcgat ctcgccgagg atgtcgtgca ggaaaccttc ctggccgcgg tcgcgggacg
     3181 cgcgagcttt tccggccgct cgagcgtcag gacctggctc accgggatcc tgaagcacaa
     3241 gatcatcgac gcgatccggc agaaacagcg ccacccgacc ctcgccgccg cgttcgacga
     3301 gacgctggac gtcgacgcgc tcgacggcat gttcaccgag gaaggcgcct ggaaaacgcc
     3361 accggccgac tggggcaacc ccgagactgc gctggcgcag agcgagttca tggacgtcct
     3421 cgcgcgctgt ctcgacaagc tcccgccggc caccgcgcgg gttttcacga tgcgcgagat
     3481 cctggagctc gaaagtgacg aaatccgtaa ggaattgtcg atcacggcga ataacctgtg
     3541 ggtgatcctg caccgcgcgc gcgtctcgct tcgccggtgc ctcgagcaga actggttcgc
     3601 ctcccctgca cgccgctgag ggccatccga tcatgctgca ccggctgacg cacgtcgttt
     3661 cctgccgcga ggcgagccgc ctcctgtcgc ggcgtgagga gcggcccctc gcgaccgtcg
     3721 agcgagtcaa gctcgcgctg catctgcgcc tgtgcaccgc ctgcacgcgc ttcgcgcgcc
     3781 agctcgcgtt catccgccgc gccctggccc gctaccgcag ctgatcatgc tcacgcttac
     3841 gctcaacggt gtggcaaccg gcgtcgagtg cgcgaccctc gccgagctcg tggcctcgct
     3901 cggactcgag ggcaagcgga ttgccgtcga gcgaaacggc gagatcgtcc cgcgcagccg
     3961 ctacgccgat accccgctcg tgcgcggcga ccggctcgag atcgtcggcg ccgtcggcgg
     4021 cggctgagag cgcaggcgta aaattcgtgc atgaacgccc ccgataatcc ggcagcgctc
     4081 gccgatccgc tgcgcattgc cggccgcgcc tatcggtcgc ggctcctcgt cggcaccgga
     4141 aagtaccgcg acttcaccga gacgcgcgat gcgctcgtcg cgtccggcgc cgagatcgtc
     4201 accgtcgcca tccggcgcac caacatcggc cagacgcccg gcgaagcgtc gcttgccgat
     4261 gcattgccgc cgtccgaatt cacgtacctg ccgaacagcg ccggctgcta cacggccgac
     4321 gacgcggtgc gcacgctgcg cctcggccgc gaattgctcg acggccacgc gctttgcaag
     4381 ctcgaggtgc tcggcgatgc gagctcgctt taccccaacg tgcgcgagac gctggtggcc
     4441 gccgagcggc tcgttgccga cggcttccag gtgatggtct acacgtccga cgatccgatc
     4501 gtcgcgcgcg agctcgaagc gatcggatgc gtcgcgatca tgccgcttgc gtcgctgatc
     4561 ggctccggca tgggaatcgt caatccgtgg aacctgcgcc tcatcctcga cgaggcgcgc
     4621 gtgccggtga tcgtcgatgc cggcgtgggt accgcatcgg atgcgacgat cgccatggag
     4681 ctcggctgtg ccggcgtgct gatgaacacg gccatcgcca aggcgcgcga gccggtgcgc
     4741 atggcgcacg cgatgaagct tgccgtcgaa tcgggacgcg aggcgtttct cgccggccgc
     4801 atgccgcgca agcactacgc gggcacgccg agctcgccca cgaccggcct catcgcttga
     4861 gcgtcgccct tcgcatgcgc cggcacgtcc gatgggcgtt cggcgcgctc ctgctgcccg
     4921 ctttcgcgca cgccgcgccg attccgcgcg gcgagctcat gacgctgtgc gccgaagccg
     4981 acgatcaggc gcattgcggc cgcctcgtcg aggcgcgtca attgcgctcc ctttcacgca
     5041 tcgccgagcg caacggcgac gagcttcggg tcgcgcttgc gccgttcggc ttgagcgtgt
     5101 ttcacgacac ggtcaacgtc aagggtgcga cgacgtatgc cgtctgggat tacctcgagc
     5161 gcgtcgacac gctcgtgctg ttcaccacgc gcggcgagcg cacgggcttc atgctcgtgc
     5221 agcgccaggg cggcggcgag taccgcgtcc cggcggagcc ggtgctcgca cccgatgatc
     5281 gccgcttcgc aaccgccgat ttctgcgcga acgagtgcga caacgaagtg gcgatctggc
     5341 acatcggcag cgatggcata cgcaaggaag cggtgtggac gccgcccgcc gcctggtccg
     5401 acgtgagcgt cgcctggcgc aacgccaatt cgattgcgct cgactatgcg ctggacgacg
     5461 gtcaacggcg cacgatcgag cggcgcctcg acgatccgtc gtggaagaag gcgcgcgcga
     5521 gatgaccggt agcgccgacc tggaacggag cgacatgagc gcgaagggcc gccccgagcg
     5581 cgaattgcgc cccctggggg gcagcgcagc ggcgaagccg caagcgcggg gggatcacct
     5641 cccccaccat cggatcagga gcttcgtgct gcgccagggc cgcatgtcgc cggcgcagca
     5701 gcgcgcctgc gacgagctgt acccgcgcta cggcgtgaac ccatccgcgc cgctcgacct
     5761 tgcggcgatc ttcggtcgtc gcgccccggt cgtgctcgag atcggcttcg gcatgggcga
     5821 gacaacggcg gcgatcgcgg cgacgcagcc cggtgtcgac ttcctcggcg tcgaaatgca
     5881 ctcgcccggc gtgggtgcgc tgctgcgccg gatcgaatcg atgcagcttg cgaacgtgcg
     5941 cgtgctgcgc cacgacgccg tcgacgtcgt cgcgcggatg attcccgctg catcgctcgc
     6001 cgggatccac gtctacttcc ccgatccgtg gccgaagaag cgccatcaca agcggcggct
     6061 cctgaagcct ccgttcgtgc acgcactcgc cgcagcactc gcgcccggcg gctacctgca
     6121 tgtcgccacc gactgggcgg cttacgccga cgagatcctc gccgcgctgg cggccgagcc
     6181 gctgctcgcg aacacggcgg ccggctttgc gccccgcccc gcgtggcggc cgctcacgaa
     6241 gttcgagcag cgcggccttg cgcgcggcca cgccgtgttc gacgtgctgt ttacttcggt
     6301 gcgagcgcaa gcagcgcggt aagcgcctcg cgcgcgatgt cgcgcgagaa gtggcgcgct
     6361 acattatcgc gcccgccggc cgacagccgc tcccacagcg cgccatcctc gtgcacgcgc
     6421 gcgatcgccg cggcgaatgc ttcgggctcg tcggcgacga gcacgtcgac accgtcggcg
     6481 aggtgcatcc cttcgacgga aaccgtcgtt gcgaccaccg gcaacccatg gctcatcgcc
     6541 gtgttgatct tgcccttgac gccggcgccg tagcgcagcg gcgagatcga cacgcggcac
     6601 gcctcgaggt acggcgcgat gtcgggcacg tagccgagca cgacgaagtc gtcggcggcg
     6661 agtgcttcga tcgacgcggt cacgcggctc ccgatcacgt aagtgctcac acctggcagg
     6721 agcctgcgca catgcggcat cacctccttc gcgtaccagc gcatcgcgtc cacgttgggc
     6781 ggatgctcga aaccgcccac gaacacgatc ccgcgtcgcg ccgcccagcc gggcacccgc
     6841 gccaccggct cgtggatcgt cgacagcagc atcacgcgcg aggccggcgc gagctccttc
     6901 agcaccgcct gctcggcgtc cgaaaccacc agcgtcacat cggccttcgc aatcaacgcg
     6961 agctcgtcgg cccgccgcga gcgcgcggaa tgcgccgcaa gacggccgcc ttcgagcttc
     7021 gcaagacgct cttcgcgcag gaagtggagg tcgaccgtat cgaaaacgat gagcgcgcgc
     7081 ggcgccgcct tgcgcacggt gtcgatgtgt cgcgcggcga tgtaatgccg cgacagcatg
     7141 atcacgtcga attcctgcgc gcgccgcatg agcagctctg aaatcgagcg cacgtaggga
     7201 tggaacagca cttccacgcc gcgctgcgtc agcgcatcga cgtacggcgc cccatgctcg
     7261 aggttgtcgg cgacgaacgt caccttgcag cgcagcgccg tggcgatctc gagcatcgcc
     7321 tgcatgcgca gcgagcccgc gtcctgatcg ggtgtcagca tgcgcgcgtc gatgacgagc
     7381 atgcgcttcg ttgcaaaccg gtcgcgctcg agctcgacat gcatgccgtt cgggcgatgc
     7441 gcgccgagcg cgctcgccca tttcgcggcg aacgccgact ggttgaccgc ctggtggcgc
     7501 tttacgccct gcgtaacgtc ggtgcccgac gtctgtccct cgaaatgcac gatcgtcgcg
     7561 gcgggttgat agacgacctt gcgtccggcg gcgcgaacgg caaaggccag atcgacgtcc
     7621 tcgtagtagg ccggtgcgta acgcgtgtcg aatccgttca attgcgcgaa cagcgcgcgc
     7681 gggatcgcga ggcacgcgcc cgagcagtag tcgacttcgc gcagatagtt gtattcgggg
     7741 cgatcgggat cgtcgccgcg gccgaagttc cacgcgctgc cgtcgcgcca cgcgatgccc
     7801 cccgcctcct gcagccgtcc gtccgcgtag acgagtcgcg cgccgacgag ccccgtatcg
     7861 gggtcctcgt ccagcacacg catcagcgcg tcgagccagc cggccgtaac gagcgtgtcg
     7921 ttgttcagga acacgagcgt gtcgccacgt gcgatcgcgg caccgcgatt gcacgtgccg
     7981 acgaagccga gattggctgc attgcgctcg aagcgcacgc cggagacctg cgccaggcat
     8041 tcggcgagcg gttgttccga tgcgtcgtcg acgacgatca cctcgaattc gccgctcgtg
     8101 tgccgcgcga tgctcgcaag gcagctgaac gtcagcagcg gctcgccata cgcgggaatc
     8161 acgatcgacg tccgcggcgc cttcgatgtc cgcagcgcaa gcggtgcaat ccgcggctcg
     8221 agccgcgcga gcgggcggat tggcggcacg aaccgcgggc gcgcgcggag cttccgcacg
     8281 acacgccgcg cgagcgccgc ggcaccatcg ttgcgcagga tcgtgagcgc cgtgcctgca
     8341 tgccgcggaa gctgccgcgc cgtacgccat tgcgtctgca atgcggcgac gacgatcttc
     8401 acgcgatgca cgccggagcg cagcggcgcg gtcatgcgcc acgtcgtcga cgactcgatc
     8461 tcgccgacgc gcctgcgcga cgcctgcgcg ctttcctcga gtgcgcgcgt gcgctggccg
     8521 agcgccgcca attggcgctc ggcctcgatg agtccaagtg cgatgccatc ccctgccgcg
     8581 tcgcggcgcc ggcgcgcctc gtcgagcgtt gctagatcgc gatcggccgc gtcgctgcgc
     8641 cggccgagct cggcacgcgc ctccgcgtgg ccgcacaacg cgccggcgac gaagagctgc
     8701 gacagcgtca gcggggcggc ttcgcgcccg acgtccggcg gctcgaaatg ccgcggatcg
     8761 aattcgccga cctcgccagt cacggcacgc ttcgacgacg cgagcggtgc catgtaacgc
     8821 tcgacgtagc gctcgcgatc catcacgatt cggcgtgaag gggtgtggcc gcgacgctcg
     8881 cgcccgcagg gtagcccacg gcttcgcgcg caacgccgat cagtgcatcg ttccagacgc
     8941 tcggcggcgc atcgaacggc agcatgcgta ccagcgcacg tccggcgaga cgctcgggaa
     9001 atgcgccgat cgccgaagcg acgatcggcg tgtcggtcgc aagcgcaacc gagagcgtgt
     9061 acgaatacgt ttccggaacc tgcgcgggaa agaacagcac gtcggcgtgc tcggcggcga
     9121 gcagcgccgg aaggcgtgct tcgacgtacg agccatgcag cgtaagcggc acgtcgggtg
     9181 attgcgcgat cggctctgcc gttgcgccga gcacgcgaaa cgcgagcggg aggccgcgag
     9241 cctgcgcctc cttcgcgcat tcggcgacga tgtgcagccc cttctccggc gaaagcccgc
     9301 cgagcgtaac gacgcgaacg atccgcggca ggggcgtggc ttcgggctcc gggtgcggcc
     9361 agacgacgat cggcagcgcc ggaaagtagc ggtgcatgcg cgctttcacg tcgtgcgacg
     9421 gcgcgatcac gcgcgcggca ctcgagaaga attcggcgaa gcgcgcgcgc cacgcatcga
     9481 tgtcgagcgg ccactgcgcg cgccggtgca cgaggcacga tgcgcaaccc gcgcgatcgg
     9541 gctcgccgca gtaacgaccg tgctcgtcgg caaggtggta ttgcgggcag atcgcgaagt
     9601 agtcgtgcag cgtgcaatcg taggcgatgc cagccgcgcg cggcagatcg aggatcgact
     9661 gaggcatccc gtgcacatgg tggaagtgga gacgcgcagc gccgatgtcg cgcagcatgc
     9721 cggccagcgc cgtcatctga tcgggcaggc ggaaccatgc ggcgaatgcc tcgccgtcac
     9781 gtggccagtg cagcctgacc gtgtcgccac ccgcgggctc gaggtacagc acgtcggccc
     9841 gctcgcgcac gagcgcggca agatcgtcca tgtagcggcg gatgccgccg ccccacgcgt
     9901 gagaaacgaa cacgattgcg ggccgcggcg aggcggcgag gcgggcaatg tcgatgcgcc
     9961 gcgcgagcgg caccgtctgg accgaggacg cgacaggcaa cgtatcgctc agcgtcgacg
    10021 ggttctgcgc aacgaacacg tcgccggcga tcagcgcacg aaagccggcg ctcgccgcgc
    10081 ggcccgtcca gtcgcgcatg acgcctgcgt cgtcgccccc catgccgatc ggcgcgccac
    10141 cggttgccgt gatcgcggcg cgcgtgatgt agcggcaggg cccctccacg gcatcgacgc
    10201 cgaccgaacg cccggcgtta acgcgcgcga acagcgcgtc gagcgaggcg gcggtctcac
    10261 cgtccggaag cgcattcgcg gtcgccggac gcggatacgc cgcggcattg gcgacgttgg
    10321 agaatgctcc gaccgcgccg atcgcgtcgc cccgcacatg cgacgcgagc cggtcgagcc
    10381 agtcgccgtg gacctcggcg ccgggcgcca tgagcacgac gtcgcggtcg cgatgcagct
    10441 cgagtacgcg ctcgagcgtc tgcgtgccgc tcgcgtcgcg ctcggccggc aacagcgtta
    10501 cgcgcgcgtt cgccgcgatg tcacgcagcg cagcggcggc gtcggcgctc gcgccggcat
    10561 ccgcgacgac aacgacatga aacggcgtca cgttcgccga cgcgagaatg gattcgacag
    10621 accggcgcag cgcgtcgatg tcgccttcgg cgggcagcac gatgtcgacg acggtggcgc
    10681 cgcgcgccgg ccgcgtggcc gcgccgctca tcgggcgggg cgcggtgacg gcgtcacgcg
    10741 agcgtcagga tgaccggctg gtggtcggcc gcctgcgtgt cgacgtcgac gcggacgtcg
    10801 cgcatgcgcg cacgcagctc ttcgccgatg aagatgaagt cgcaatggag ctcgggctcg
    10861 tcggggcgct ttttctcgta gatcttgaac gtcggcggat gcgccctgcc gggctgaagc
    10921 gcggaccacg cgtcgacgag cgcgggcgtg ccgtcggcga atggcgcgag catgcgccgg
    10981 tgctcgggcg aatcgggctc gaaattgcag tcgccggtga tcacggtttc ccgcggccgg
    11041 atgaacgtct ggaacgggcc gccatcgctc gtcgtcacct gcgcgccgcg tgcgtacgca
    11101 tggccctcgg cgtagatcgc gcgcagcgcc tcgacctgcg ccatgcgctg cttcgccgaa
    11161 taatattcga ggtgcgtcgt aatcactcgc acatcatggc cgtcttcgcc gaggaccgcc
    11221 tcgacggcga tgcgcggcat gccgggcacg cccgggtcgc acggatacgg cagcaggtgc
    11281 cgatacacct ggcgcaccgg caatcgcgaa aggatcatgt tgccgaagcg ccgccggcgt
    11341 ccgtcgtcgc ccggctggtc gactgcaatg ccgggcacgg cggtgaaccc gggaagcagt
    11401 tgcgcaagtc gcgcgaactg gtcctcgccg tcgctcccgg cgagccgcgg gtcgggatag
    11461 ttgtcggcga tctcctgcag gcacaggacg tcgaaatcgg cgagccgccg cgcttccgcg
    11521 acgatgcgcg ccggatcgac ggcaccgtcg acgccgcggc accactgcac gttccaggtg
    11581 atcagtttca tggtcgtacg gttccgtggg gtccagataa cgcaaaagcc gaggggatcg
    11641 gcgctgccag gtttgcccgg at
//
LOCUS       DASSVX010000155         8592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_822641, whole genome shotgun sequence.
ACCESSION   DASSVX010000155 DASSVX010000000
VERSION     DASSVX010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822641"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..104)
                     /locus_tag="VFO53_06770"
     tRNA            complement(30..104)
                     /locus_tag="VFO53_06770"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70..72),aa:Val,seq:cac)
     gene            346..1773
                     /locus_tag="VFO53_06775"
     CDS             346..1773
                     /locus_tag="VFO53_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET8876015.1"
                     /translation="MSSPAYDEMRTGDGAIRSHYRAFADWLHRTPPDRIAQKREEAER
                     LFNRVGITFAVYGEQAGTERLIPFDVVPRIIPAHEWQRLEAGLVQRVQALNLFLQDIY
                     HEQAILRAGIIPSQCVLANSQYRREMMGVDVPGGIYAHVAGIDVVRDEHGEYCVLEDN
                     LRVPSGVSYMLENRKMTMRLFPELFASQSIRPIQHYPDVLLESLHAVAPAGRERPTVA
                     VMTPGAFNSAHFEHAFLAQQMGVELVEGQDLFVQDDTVYMRTTRGPQRVHVLYRRVDD
                     DFLDPLAFRPDSMLGVPGLFAAYRAGRVTLANAIGTGVADDKSIYPYVPDMIRFYLTE
                     EPLLANVPTLVLARADDRAQALAHLSDLVVKEVHGAGGYGMLIGPAASAAERDAFRRR
                     IEAAPERYVAQPTIALSTCPTFAASGLAARHIDLRPYVLCGKGIGLVPGGLTRVALPE
                     GSLVVNSSQGGGTKDTWVLAPEEPC"
     gene            1793..2746
                     /locus_tag="VFO53_06780"
     CDS             1793..2746
                     /locus_tag="VFO53_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HET8876016.1"
                     /translation="MLSRHANELYWMARHIERAENTARLLDVTYRTSLLPYQVGEPGL
                     AWAAPWSVPLITLGVASTYYERYPVLTAENALRFMILDPSNTSSIYACLGAARESART
                     VRGAITSEMYEDLNSAWIEMRTCDYERIQARGVSDFLDWVKMRSHLFRGVTFGTMLRD
                     EAYHFIRLGTHMERADNTARILDTKYHILLPSVADVGGAVDYYQWASLLQSLSGFEAY
                     RKIYSDVISPRRIAELLILRDDMPRSLHSCMNFIHETLQLLCDAQSREIERQAGEMHA
                     RIHYGRTDDIMDFGLHEYLTEFLEHISRLGAEVSRTFLVPV"
     gene            2839..3552
                     /locus_tag="VFO53_06785"
     CDS             2839..3552
                     /locus_tag="VFO53_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HET8876017.1"
                     /translation="MSLDAGMIFASDSRTNAGVDHIARFSKMRVFARDADRVVVMLSS
                     GNLSVTQSAINILEQQARGGDAEQHVWTATSMFEVARLLGDALREVRARDGPYMAQNN
                     IDASANFIVGGQVHGEAPRLFEVYSEGNFIEATPDTCYFQIGESKYGKPVIDRVVHRG
                     TTLLDATKCTIVSFDSTMRSNISVGLPIDLLVYETGSLKVTLQRRIQEFDPYFQLIHH
                     QWGEGLRRVFAQLPDPDWL"
     gene            3573..6650
                     /locus_tag="VFO53_06790"
     CDS             3573..6650
                     /locus_tag="VFO53_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET8876018.1"
                     /translation="MPIRVELTHCTRYRYDRPVTLSAHEIRLRPAPHCRTPIGAYSLA
                     IEPAEHFVNWQQDPYGNFVARVVFPAMTDRLVVSVDLHAELTVINPFDFFVEPYAEAF
                     PFAYAPALEKELAPFLEAPREGERFDRWVARFRETMRADISTVNLLVEVNQRVKDAIA
                     YVVRMEPGVQSPDATLAGASGSCRDSAWLLVQVLRRLGIAARFASGYLIQLAPDVKPL
                     DGPAGVERDFTDLHAWAEAYVPGAGWVGLDPTSGLLAGEGHIPLACTASPGSAAPITG
                     LADVADVQFDFTMGVTRIAEHPRVTRPYTEAQWQAIDALGERVDAKLAAADVRLTQGG
                     EPTFVAIDDPDAPEWNITALSPAKRERAETLVRRLAARFAPGGLLHHGQGKWYPGEPL
                     PRWALGIYFRADGVPLWRDAALLADVRTRGACVLDDARRFVDTLVHELRLPSGNVLPL
                     YETPGGSITVSDGPVGFVLPLAAGDESEGEERAWRTCAWPLPQGRLHAVAGDSPLGLR
                     LPLASLPATLPVRTALAVELRNGHVAVFLPPVARVDDSVQLIAAVEYSARAVGKPLLV
                     EGYPPPRDPRIRALLVTPDPGVIEVNIHPASSWRELVVSTQALYDEARATRLATEKFM
                     LDGRHAGTGGGNHITLGGATAADSPLLRRPDLLQSLVTYWQNHPALSYLFSGSFIGPT
                     SQAPRVDEARDDRLYELAIAFQQLERLQAPAKAAKPEFVDKLLRHLLTDLTGNTHRAE
                     FSIDKLFSPDTPTGRLGLLELRAFEMPPHARMAAVQMLLLRALVVRFWRVPYRAALVP
                     WGTALHDRWLLPHFVMADLADVVADLARHDLDLAPEWFAPFAEFRFPRFGSVAYDGIT
                     LELRQAIEPWHVLGEEVHRTGTARYVDSSVERLQIKVAGMVAGRHGVTCNGRALPMTP
                     TGIPGEFVAGVRFKAWNPPSSLHPAIGVQAPLRFELVDRWAQRAIGGCTYHVAHPGGR
                     NYDTFPVNANEAESRRLARFWPHGHTPGPLTVHDEPPNPSLPTTLDLRWNPSS"
     gene            6632..8056
                     /gene="gltX"
                     /locus_tag="VFO53_06795"
     CDS             6632..8056
                     /gene="gltX"
                     /locus_tag="VFO53_06795"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET8876019.1"
                     /translation="MEPLIVTVRTRFAPSPTGFLHLGNIRSALFPWAFARHHRGTFIL
                     RIEDTDQERSTPEATQSIVETMHWLGLDYDEGPYYQMQRMERYRAVLDEMLAHGLAYR
                     CYTTPAELEQLRAEQMARGEKPRYDGRWRPENAHGKTPPDGIAPVFRFRNPDDGVVAW
                     DDAVKGSIEIANGELDDLVIARADGTPTYNFCVVVDDVDMRITHVIRGDDHVNNTPRQ
                     INIMRALGATPPVYAHLPTVLTPAGERLSKRHGARGVLQYRDEGYVPEAIVNYLARLG
                     WAHGDAEVFGVDEFVAWFNLQGLTPSPARFDPGKLEWLNHEHLKRLDDAELGRRLAPF
                     LAHVGVATEGGPGAERVAALLRDRSATLSAMAEAARYFYEAPDVDAAMLAAQIDDANR
                     GALVELHDALASVPWERAALGAAIKSVASRHGLKPALVMMPLRALVAGTLKTPAIDAV
                     LELVGRDATRERMARGLRLAASSL"
     gene            8159..>8592
                     /gene="pyrG"
                     /locus_tag="VFO53_06800"
     CDS             8159..>8592
                     /gene="pyrG"
                     /locus_tag="VFO53_06800"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953152.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HET8876020.1"
                     /translation="MTKFVFVTGGVVSSLGKGIAAASLAAILASRGVRVTNLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTDVRMGKRNNFTTGQIYESVIRKE
                     RRGDYLGGTVQVIPHITDEIKGWIHSGAGDAEVAVVEVGGTVG"
BASE COUNT         1350 a         2983 c         2786 g         1473 t
ORIGIN      
        1 tacactcgcg cgtaacccct tggttcatgt ggtgggcgat actgggttcg aaccagtgac
       61 ccctgccgtg tgaaggcagt gctctaccgc tgagctaacc gccccgcgaa cccagacagg
      121 agccgcgatt taaccatagg cgggggggta gttcaaaccg gttttgacaa tttgtcgaac
      181 cgtgtcctgg acgttaacgc actaaagcgg tgcagctcgt cctcgagcca cgggcggcat
      241 cgtgtcctga ccgtcgtcct gcgaccgggc cacattgttt cggaacgctt ttagctctcc
      301 ggtccgcttc ttgcttgtag tgaacgactc tcctgtacac ccgtgatgtc gtccccggcc
      361 tacgacgaaa tgcggaccgg cgatggcgcc atccggtcgc attaccgtgc gtttgccgac
      421 tggctgcacc gcacgccccc cgaccgcatt gcgcagaagc gcgaagaggc cgagcgcctg
      481 ttcaaccgcg tcggcatcac gttcgccgtg tacggcgagc aggccggcac cgagcggctg
      541 attcctttcg acgtcgtgcc ccgcatcatt cctgcgcacg aatggcagcg gctcgaagcg
      601 ggcctcgtgc agcgtgtgca ggcgctgaac ctttttctgc aggacatcta ccatgagcag
      661 gcgattcttc gcgccgggat catcccgtcg caatgcgtgc tcgccaattc gcaataccgg
      721 cgggagatga tgggcgtcga cgtgcccggc ggcatctacg cccacgtcgc gggcatcgac
      781 gtcgtccgcg acgagcacgg cgagtattgc gtgctcgagg acaatctgcg cgtgccctcg
      841 ggcgtgtcgt acatgctcga gaaccgcaag atgacgatgc ggctcttccc cgagcttttc
      901 gcatcgcagt cgatccggcc gatccagcac taccccgacg tcctgctcga gagcctgcac
      961 gcggtggcgc ccgccggtcg cgagcggccg accgttgcgg tcatgacgcc cggcgcgttc
     1021 aactcggccc atttcgagca cgcgttcctt gcgcagcaga tgggcgtgga actcgtcgaa
     1081 ggccaggacc ttttcgtgca ggacgacacc gtctacatgc gcacgacacg cggcccgcaa
     1141 cgcgtccacg tgctgtaccg gcgcgtggac gacgacttcc tcgacccgct cgcgtttcgg
     1201 ccagattcga tgctcggcgt gcccggcctc ttcgccgcct atcgcgccgg ccgggtgacg
     1261 ctcgccaatg cgatcggcac cggcgttgcc gacgacaagt cgatctatcc gtacgtgccc
     1321 gacatgatcc ggttctatct gaccgaggag ccgctgctcg ccaacgtccc gacgctggtg
     1381 cttgcgcgcg ccgacgatcg cgcgcaagcg ctggcgcatc tttccgatct cgtggtcaag
     1441 gaggtgcacg gcgcgggcgg ctatggaatg ctgatcggtc ccgccgccag cgcggcggag
     1501 cgcgacgcct ttcgccggcg catcgaggcc gcgcccgaac gttacgtggc acagccgacg
     1561 atcgcgcttt cgacctgccc gacgttcgcg gcgagcggac tcgcggcgcg ccacatcgat
     1621 ctgcggccgt acgttctgtg cggcaagggc atcgggctcg tccccggcgg gctcacccgc
     1681 gtcgcactgc ccgaaggctc gctcgtcgtc aattcgtcgc agggcggtgg caccaaggac
     1741 acctgggtgc tcgcgccgga ggagccgtgc tgaggccgcg gcgcaccgca tcatgctgag
     1801 ccggcacgca aacgagctgt actggatggc gcgccacatc gagcgcgccg agaacacggc
     1861 gcgcctgctc gacgtcacgt accgcacgtc gctcttgccc taccaggtcg gcgagcccgg
     1921 gctcgcctgg gcggcgccgt ggtcggtgcc gctgatcacg ctcggtgtcg cctcgaccta
     1981 ttacgagcgg tatccggtgc tgacggcgga gaacgcgctg cgcttcatga tcctcgatcc
     2041 gtcgaacacg tcgtcgatct acgcgtgcct cggtgccgcg cgcgagtcgg cacgcaccgt
     2101 gcgcggcgcc atcacctccg agatgtacga ggatctcaac tcggcgtgga tcgagatgcg
     2161 tacctgcgac tacgagcgca tccaggcgcg cggcgtgtcc gactttctcg actgggtcaa
     2221 gatgcgctcg cacctgtttc gcggcgtcac gttcggcacg atgctgcgcg acgaggcata
     2281 ccacttcatt cgtctcggca cgcatatgga acgcgccgac aataccgcgc gcatcctcga
     2341 cacgaaatac cacatcctgc tgccgtccgt ggccgacgtc ggcggcgccg tcgattacta
     2401 tcaatgggcg tcgctgctgc agtcgctgtc ggggttcgag gcgtaccgca agatctacag
     2461 cgacgtcatc tcgcctcggc gcatcgccga gcttctgatc cttcgcgacg acatgccgcg
     2521 ctcgctccat agctgcatga acttcatcca cgaaacgctg caactgcttt gcgacgcgca
     2581 aagccgcgaa atcgagcggc aggcgggcga gatgcatgcg cgcatacact acggccgtac
     2641 cgacgacatc atggacttcg gcctgcacga atacctgacc gagttcctgg aacatatctc
     2701 gcgcctcggc gccgaagtga gcaggacgtt cctcgtgccc gtgtaatctc ccgctcggtg
     2761 cttgccctcc ataaacccct tggctcatga ggttgcaatg aacattgcat ccgccttctc
     2821 atgacttatt gcgtcgccat gtcgctggac gccgggatga tcttcgcgtc cgattcgcgc
     2881 acgaatgccg gcgtcgacca tatcgcgcgc ttttccaaga tgcgcgtatt cgcgcgcgac
     2941 gccgatcgcg tcgtcgtcat gctgtcgtcc ggaaacctct cggtcacgca gagcgcgatc
     3001 aacatcctcg agcagcaggc gcgcggcggc gacgccgagc agcatgtctg gaccgcgacg
     3061 tcgatgttcg aggttgcgcg tcttctgggc gacgcgctgc gcgaggtgcg cgcgcgcgac
     3121 gggccgtaca tggcgcagaa caacatcgac gcgtcggcga atttcatcgt cggcggccag
     3181 gtgcacggcg aggcgccgcg gctgttcgag gtctacagtg agggcaattt catcgaggcg
     3241 acgcccgaca cctgctactt ccagatcggc gaatcgaagt atggcaagcc ggtgatcgac
     3301 cgcgtcgtcc accgcggcac gacgctgctc gacgcaacca aatgcacgat cgtgtcgttc
     3361 gattcgacga tgcgctcgaa catttccgtc ggcctgccga tcgacctgct cgtctacgag
     3421 acgggcagcc tcaaggtgac gctgcaacgg cgcatccagg aattcgatcc ttacttccag
     3481 ctgatccacc accagtgggg cgagggcctg cgccgggtgt tcgcgcagct tcccgatccc
     3541 gactggctct gatcgaagcg ggcgcgcacg ccatgccgat ccgggtcgag ctcacgcatt
     3601 gcacgcgtta tcgctacgac cggccggtga cgctttcggc gcacgagatc cggctgcggc
     3661 cggcgccgca ttgccgcacg ccgatcggtg cgtattcgct tgcgatcgag cccgccgagc
     3721 atttcgtcaa ctggcagcag gacccgtacg gcaacttcgt ggcgcgcgtg gtgtttcccg
     3781 cgatgacgga tcggctcgta gtcagcgtgg acctgcacgc cgaattgacg gtgatcaacc
     3841 cgttcgactt cttcgtcgag ccgtacgccg aagcgttccc gttcgcgtat gcgccggcgc
     3901 tcgagaagga gctcgcgccg tttctcgaag cgcctcgcga gggcgagcgc ttcgaccgct
     3961 gggttgcgcg cttccgcgaa acgatgcgcg cggacatctc gaccgtgaac ctgctcgtcg
     4021 aggtgaacca gcgcgtgaag gacgccatcg cctacgtcgt gcgcatggag ccgggcgtgc
     4081 agtcgcccga cgcaacgctc gccggggcca gcgggtcgtg tcgcgattcg gcgtggcttc
     4141 tcgtgcaggt gctgcgccgg ctcggcattg ccgcgcgttt tgcctccggc tacctgatcc
     4201 agctcgcgcc cgacgtcaag ccgctcgacg ggccggcggg tgtcgagcgc gatttcaccg
     4261 acctgcacgc gtgggccgag gcctacgtgc cgggcgcagg ctgggtgggg ctcgacccga
     4321 cgtccggctt gctcgccggc gaaggccaca tcccgctcgc ctgtacggcc agccccggca
     4381 gcgcggcgcc gatcacgggt ctcgccgacg ttgcggacgt gcagttcgac ttcacgatgg
     4441 gcgtgacgcg catcgccgag catccgcgcg tgacgcgtcc gtacaccgag gcgcaatggc
     4501 aggcgatcga tgcgctcggc gagcgcgtcg acgcgaagct cgccgcggcc gacgtgcgcc
     4561 tgacgcaggg cggcgagccc accttcgtgg cgatcgacga tcccgacgcg cccgaatgga
     4621 acatcaccgc attgtcgccg gcgaagcgcg agcgggccga aacactggtg cggcggttgg
     4681 cggcgcgctt tgcgccgggc ggactcttgc atcacggaca gggcaagtgg tacccgggcg
     4741 agccgttgcc gcgctgggcg ctcggcatct actttcgtgc cgatggcgtg ccgctgtggc
     4801 gcgatgcggc gttgctggcc gatgtgcgaa cgcgcggtgc atgcgtgctc gacgatgcgc
     4861 gccgtttcgt cgatacgctg gtgcacgagc tgcgcctgcc gagcggcaac gtgttgccgc
     4921 tgtacgagac ccccggcggg tcgatcacgg tgtccgacgg gcccgtcgga ttcgtgctgc
     4981 ccctcgccgc gggtgacgaa tccgaaggcg aagaacgcgc gtggcggacc tgcgcatggc
     5041 cgctccccca aggtcgcctg catgcggtgg ccggcgattc gccgctcggg ttgcgcctgc
     5101 ctcttgcgtc gctgcccgcg acgctcccgg tgcgcacggc actcgccgtg gagcttcgca
     5161 acggacacgt cgccgtcttc ctgccgcccg ttgcacgcgt cgacgactcg gtgcaactga
     5221 tcgccgccgt cgagtacagc gcgcgagcgg tgggcaagcc gctgctcgtc gaaggctatc
     5281 cgccgccacg cgacccgcgc atacgggcct tgctcgttac gcccgatccc ggcgtcatcg
     5341 aggtgaacat tcatccggcg tcgagctggc gcgagctcgt cgtctcgacg caagcgctgt
     5401 acgacgaggc gcgcgccacg cgcctcgcca ccgaaaagtt catgctcgac ggccgccacg
     5461 cgggcaccgg cggcggcaat cacatcacgc tcggcggtgc gactgccgcc gacagtccgc
     5521 tgctgcgccg tcccgatctg ctgcaaagcc tcgtcacgta ttggcagaac catccggcgc
     5581 tgtcgtatct tttctccggc agcttcatcg gacccaccag ccaggcgccg cgcgtcgacg
     5641 aagcacgcga cgatcgtctc tacgagctcg caatcgcgtt ccagcaactc gagcgcctgc
     5701 aggcacccgc aaaggcagcg aagccggaat tcgtcgacaa gctgttgcgg catctgctga
     5761 ccgatctcac cggcaacacg caccgcgccg agttctcgat cgacaagctc ttttctcccg
     5821 acacgccgac cggccgcctc ggcctcctgg aattgcgcgc cttcgaaatg ccgccgcacg
     5881 cacggatggc ggccgtgcag atgctgctgc tgcgcgcact ggtcgtgcgt ttctggcgcg
     5941 tgccgtaccg tgccgcgctc gtgccctggg gcacggcgct gcacgaccgg tggctgctgc
     6001 cgcatttcgt gatggcggac ctcgccgatg tcgtcgccga tctcgcacgc cacgacctcg
     6061 accttgcgcc ggaatggttc gctccgttcg ccgaattccg ctttccgcgc ttcggcagcg
     6121 tcgcctacga cggcatcacg ctcgagctgc ggcaggcgat cgagccctgg cacgtgctcg
     6181 gcgaagaggt ccaccgaacc ggcaccgcac gctacgtcga ctcgtcggtg gagcggctgc
     6241 agatcaaggt ggcgggaatg gtggcgggtc gccatggcgt cacgtgcaac ggacgcgcgc
     6301 tgccgatgac gccgaccggc attcccggcg aattcgtcgc cggcgtgcgc ttcaaggcat
     6361 ggaatccgcc gtcgtcgctg catccggcca tcggcgtgca ggcgccgtta cgcttcgagc
     6421 tcgtcgatcg ctgggcgcag cgcgccatcg gcgggtgcac ctatcatgtt gcgcatccgg
     6481 gtggccgcaa ctacgacacg tttccggtga acgcgaacga ggccgagtcg cgccggctcg
     6541 cgcgcttctg gccgcacggt catacgcccg gaccgctcac cgtgcacgac gagccgccga
     6601 atccgtcgct gccgaccaca ctcgacttgc gctggaaccc ctcatcgtga ctgtccgcac
     6661 ccgcttcgca ccgagtccca cggggtttct gcacctcggc aacatccgct cggcactttt
     6721 cccgtgggcg ttcgcgcgtc accaccgcgg cacgttcatc ctgcgcatcg aggacaccga
     6781 ccaggagcgc tcgacgcccg aagcgacgca gtcgatcgtc gaaacgatgc actggctcgg
     6841 gctcgattac gacgagggac cttattacca gatgcagcgg atggagcgtt accgcgcggt
     6901 gctcgacgag atgctcgcgc acgggctcgc gtatcgctgc tacacgacgc ccgccgagct
     6961 cgagcaactg cgcgccgagc agatggcgcg cggcgaaaag ccacgctacg acgggcgctg
     7021 gcgtccggag aacgcgcacg gaaagacgcc gcccgacggc attgcgccgg tgtttcgctt
     7081 ccgcaatccg gacgacggtg tcgtcgcctg ggacgacgcg gtcaagggtt cgatcgagat
     7141 cgccaacggc gagctcgacg acctcgtcat cgcgcgtgcc gacggcacgc cgacctacaa
     7201 cttctgcgtg gtcgtcgacg acgtcgacat gcgcatcacg cacgtgattc gcggcgacga
     7261 ccacgtgaac aacaccccgc ggcagatcaa catcatgcgc gcgctcggcg cgacgccgcc
     7321 cgtctacgcg catttgccca ccgtcctcac acccgccggc gagcggcttt ccaagcgaca
     7381 cggcgcgcgc ggcgtgttgc agtatcggga cgagggctac gtcccggaag cgatcgtcaa
     7441 ctatctcgcc cggctcggct gggcgcatgg cgatgccgaa gtcttcggcg tcgatgaatt
     7501 cgtcgcctgg ttcaacctgc aggggctgac gccgtcaccg gcacgcttcg accccggcaa
     7561 gctcgaatgg ctcaatcacg agcatctcaa gcgcctcgat gacgccgagc ttgggcgccg
     7621 cctcgcgccg ttcctcgcgc acgtgggcgt cgcgaccgaa ggcggtccgg gcgcggaacg
     7681 cgtggcggcg ctgttgcgcg atcggtcggc gacgttgagc gcgatggcgg aggctgcgcg
     7741 ctacttctac gaggcgcccg acgtcgacgc agcgatgctc gcagcgcaga tcgacgatgc
     7801 gaatcgcggg gcgctcgtcg aactgcacga cgcactcgcg tcggtgccgt gggagcgtgc
     7861 cgcattgggc gctgcgatca agtcggtcgc gtcccgccat gggctgaagc ccgcgctggt
     7921 gatgatgccg ctgcgcgccc tcgttgccgg taccctcaag acgccggcga tcgacgccgt
     7981 gctggaactc gtcgggcgcg atgcgacgcg agaacgcatg gcgcgcggcc tgcggctcgc
     8041 cgcctcctcg ttgtagcgcg cgcagcacgc ggctcgcgtt cgcagacaat tcgtcgcgca
     8101 cgctttggcg ccgcggcggc gttccggtaa aataccaatt tccggaactg gccagccaat
     8161 gaccaagttc gtgttcgtga cgggcggcgt cgtttcctcg ttggggaagg gcatcgccgc
     8221 cgcgtcgctc gccgccatcc tcgcctcgcg cggcgtccgc gtcaccaatc tcaagctcga
     8281 tccgtacatc aacgtcgacc ccggcacgat gtcgccgttc cagcacggcg aggtgttcgt
     8341 gaccgaggac ggcgccgaga ccgacctgga cctcggccac tacgagcgct tcaccgacgt
     8401 gcggatgggg aagcgcaaca acttcaccac cggccagatc tacgagagcg tcatccgcaa
     8461 ggagcgccgc ggcgactacc tcggcggcac cgtgcaggtg atcccgcaca tcaccgacga
     8521 aatcaagggc tggatccaca gcggtgccgg cgatgccgag gtcgccgtgg tcgaagtcgg
     8581 cggcacggtc gg
//
LOCUS       DASSVX010000156         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_956077, whole genome shotgun sequence.
ACCESSION   DASSVX010000156 DASSVX010000000
VERSION     DASSVX010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956077"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VFO53_06805"
     CDS             complement(<1..1055)
                     /locus_tag="VFO53_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8876021.1"
                     /translation="MKSGPDTPHPNPPGASEAGDGSDRANILIVDDRPDKLLVYLTLL
                     EELQQNLYTASSGDDALRQVLDREFALILLDVNMPGLDGLETAALIRSRRKSAHTPII
                     LVTADYGDELRMTKAYALGAVDYIASPVVPEILRAKVRVFVDLYLLAEQAKRQAQERI
                     ALIEEKAAREAAERASRRLAFLAEASATLASSLDLDATMREVSQLVVPRFADVAIVSL
                     VSSEGQTERHEMAWATGDTENPLLTASLAALDDSMLDDAVARVRDSGKPESIERDPAG
                     PPASRIALPRGLAVHTLMLVPLLVRGRKLGVLTLGLDGAARRFDADALAMVTDLATRG
                     ATALDNALLFRKIQDED"
     gene            complement(1052..>2536)
                     /locus_tag="VFO53_06810"
     CDS             complement(1052..>2536)
                     /locus_tag="VFO53_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8876022.1"
                     /translation="LLGGEIRLSSVPRSGSTFTLFLPLTYTPPRSLRKVAGERAYGDA
                     PGRHAASVNTPPVARIVEVPPVPIGDVHSRRAPAVPPPALDSPGEDADDIGDDRHSIG
                     SSDRVLLIVENDVSFARLLLEAARAKGFKGIVTTSGVSALALAAEFVPAAVTLDIFLP
                     DIDGWRVLDRLKNDDQTRHIPVSVISTDESRHRALKSGAFQFAPKPMQSAEDIDVLLD
                     RLSGYLQRTRRHLLAVSADAGKRAWFVEHLGAPDVKVTSVAGYAEAATVLRAEPVDCL
                     VLDAALDAPSDAMFMQEGNGSVAPDAPPVLLYGDRHDVGEFDHWGAFAKRADVREVRS
                     AERLLEQASHCMHRQEAAMPEMHRATLKSLQDANEVLQGRKVMIVDDDMRNIFALTSL
                     LEDRGMVIVSHDNGRDAVSALHEQPGVEAILMDIMMPEMDGIDTIREIRQIPACRDTP
                     IIAVTAKAMKGDREKCMEAGAWDYLSKPVDTELILGCLRGWLSR"
BASE COUNT          399 a          869 c          846 g          422 t
ORIGIN      
        1 tggtcctcgt cctggatctt gcggaagagc agcgcgttgt cgagcgcggt tgcgccgcgc
       61 gtggcgaggt cggtcaccat ggcgagcgcg tcggcgtcga agcggcgggc cgcgccgtcg
      121 agcccgagcg tgagcacgcc gagcttgcgt ccacgtacaa gcagcggcac gagcatcagc
      181 gtgtgcacgg caagaccacg cggcagcgcg atgcgcgaag cgggcggacc ggccgggtca
      241 cgctcgatcg attcgggctt gccggagtcg cgcactcgcg ccaccgcgtc atcgagcatc
      301 gaatcgtcga gcgctgcaag cgacgccgtc agcagcggat tttcggtgtc gcccgtcgcc
      361 cacgccatct cgtggcgctc ggtctgcccc tcgctcgaca cgagcgagac gatggcaacg
      421 tcggcgaagc gcggcacgac cagttgcgac acctcgcgca tcgtcgcgtc gagatcgagc
      481 gagctcgcga gcgttgcact cgcctcggcc aggaacgcga gccgccgcga agcgcgctcg
      541 gccgcctcgc gtgccgcctt ctcctcgatc agcgcaatac gctcctgcgc ctgacgcttc
      601 gcctgctcgg cgagcaggta caggtcgacg aacaccctca ccttcgcccg aaggatctcc
      661 ggcaccaccg gcgaggcgat gtagtcgacg gcgccaagcg catacgcctt cgtcattcgc
      721 agctcgtcgc catagtcggc ggtgacgagg atgatcggcg tgtgcgccga tttcctgcga
      781 ctgcggatca gcgcggcggt ctcgagtcca tcgaggccgg gcatgttcac gtcgagcagg
      841 atcagcgcga attcgcggtc gagcacctgt cgcagtgcat catcgccgga cgacgccgta
      901 tagagattct gctgcaactc ctcgagcagc gtcaggtaga cgagcagctt gtccggccga
      961 tcgtcgacga tcaggatgtt cgcgcgatcg gatccatcac ccgcctcgct cgcgcccggc
     1021 gggttggggt gaggggtgtc cggcccgctt ttcatctcga cagccacccc cgcagacaac
     1081 cgaggatcag ctcggtatcc accggcttcg acaggtaatc ccaggcgccc gcctccatgc
     1141 acttttcgcg gtcgcccttc atcgccttcg cggtcacggc gatgatcggc gtatcgcggc
     1201 acgcaggtat ctggcgaatc tcgcggatcg tatcgatgcc gtccatctcg ggcatcatga
     1261 tgtccatcag aatggcctcg acccccggct gctcgtgcag tgcgctgacc gcatcgcgcc
     1321 cgttgtcgtg tgaaacgatc accatgccgc gatcctcgag cagcgacgtc agcgcgaaga
     1381 tgttgcgcat gtcgtcgtcg acgatcatca ccttgcgccc ctgcagcacc tcgttcgcat
     1441 cctgcagcga cttcagcgtt gcgcggtgca tttcgggcat cgccgcctcc tggcgatgca
     1501 tgcagtggct cgcctgctcg agcagccgct cggccgagcg cacctcgcgc acgtcggcgc
     1561 gcttggcgaa cgcgccccag tggtcgaatt cgccgacgtc gtgccggtcg ccgtatagaa
     1621 ggacgggcgg cgcgtcgggc gcgacgctgc cattgccctc ctgcatgaac atcgcgtcgg
     1681 acggcgcatc gagcgccgcg tcgagcacga ggcagtcgac cggctcggcg cgcagcacgg
     1741 ttgccgcctc ggcgtagccc gcaacgctgg tgaccttgac gtcgggggca cccagatgct
     1801 cgacgaacca cgcacgcttg cccgcgtcgg ccgatacggc cagcagatgg cgtcgcgtgc
     1861 gctgcagata gcccgacaac cggtcgagca gcacgtcgat gtcctcggcg ctttgcatcg
     1921 gcttcggcgc gaactggaac gcgcccgact tgagtgcccg gtggcgcgac tcgtcggtgg
     1981 aaatcaccga cacgggtatg tggcgcgtct ggtcgtcgtt tttgagtcga tcgaggacgc
     2041 gccagccgtc gatgtcgggc aggaagatgt cgagcgtgac ggcagccggc acgaattcgg
     2101 cggccagcgc gagtgccgat acgccggacg ttgtcacgat gcccttgaac cctttcgcgc
     2161 gtgccgcttc cagcaatagc cgcgcgaagc tgacgtcgtt ctcgacgatc agcagcacgc
     2221 ggtcggagga cccgatcgaa tgacgatcgt cgccgatgtc gtccgcatcc tcgcccggcg
     2281 agtcgagcgc tggcggcggc acggcaggtg cacgccgcga gtgtacgtcg ccgatcggca
     2341 ccggcggcac ctcgacgatc cgcgccaccg gtggcgtgtt gacgcttgct gcatgacggc
     2401 cgggcgcgtc gccatacgcc cgctcaccgg cgaccttgcg cagcgatcgt ggcggcgtgt
     2461 aggtgagcgg caagaacagc gtgaaggtgc tgccgctgcg cggtacgctc gacaggcgaa
     2521 tctcgccgcc gagcag
//
LOCUS       DASSVX010000157         4553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_339524, whole genome shotgun sequence.
ACCESSION   DASSVX010000157 DASSVX010000000
VERSION     DASSVX010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339524"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFO53_06815"
     CDS             complement(<1..264)
                     /locus_tag="VFO53_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HET8876023.1"
                     /translation="MTPRYNSGFGSEFATEALAGALPVGRNSPQRPAFGLYAEQLSGT
                     AFTVPRHANRRSWLYRIRPALLHGTFEPLPQPRLATAPFDATQT"
     gene            complement(261..1298)
                     /locus_tag="VFO53_06820"
     CDS             complement(261..1298)
                     /locus_tag="VFO53_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET8876024.1"
                     /translation="MDSVDLDVLKKCDEWLEAGHRALLVTVVKTWGSSPRPDGAMLAV
                     RDDGLAAGSVSGGCIEDDLIERMRHDGFQQTRCDVVTYGVSADEARRFGLPCGGTIRL
                     VLEPLTRESGIRALLREIEAGHLTARRLDLATGRATLHPARASDGQSFDGAVLTTIHG
                     PRYRMLVIGASQLSKYLAQIAIGLDYQVTVCDPRDEYMETWDVPGVTLVRTMPDDTVQ
                     ALALDERCAVVALTHDPKLDDLALMEALKSPAFYVGAIGSRANNEKRRERLREFDLSD
                     AEIARLHGPVGLYIGSRTPPEIAISILAEVTAIKNGVDASRIVSVAAGKDTRAQTPAC
                     SVVDEAQRADG"
     gene            complement(1323..1928)
                     /locus_tag="VFO53_06825"
     CDS             complement(1323..1928)
                     /locus_tag="VFO53_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HET8876025.1"
                     /translation="MELSNTRTVPLPREVVWNALNDPAVLKDCVPGCESMEPDGEHAW
                     RVVMAARVGPVSARFNGRMAMTDINEPESYTLQFDGQGGAAGFARGDARVTLKPLDER
                     QTEMSYVAKAQIGGKLAQIGSRLVDGAAAKLADDFFARFVARAAPAATSAATSAASSE
                     VAAVDDTSTPLAPAGGSPWIRYAAIVAIVVVLVVLYVRGYR"
     gene            complement(1952..3160)
                     /locus_tag="VFO53_06830"
     CDS             complement(1952..3160)
                     /locus_tag="VFO53_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET8876026.1"
                     /translation="MSPPLARAAGHLADNVVYFVRMLRVAGLPVGPAKVIDALAAVEA
                     VGIGNRDDFREALAAVVVSKREHLVLFEQAFDLFWRNPRLLEKMLAALLPQVQGRGGD
                     ADELPARLAQALLPPPPPRHESREDESNLDAAFSFSAREVLQKKDFATMSVAELAEVK
                     ALLRRMKLPLPQIAVRRTIASPRGHRIDLRATLRRSVGAGGALTPLQFRARRMRTPPL
                     CVLCDISGSMDRYARMLLHFLHAITNDRHRVHVLTFGTRLTNITRHLRHRDVDVALAH
                     VAGAIEDWAGGTRIGQCLGDFNRLWSRRLLGQNAVVLLISDGLDADAGDGLAFEAERL
                     SKSCARLVWLNPLLRYAGFEARPAGIRALLPHVDDFLPVHNLETLTDLTHALAKRPRR
                     ERGVPLPAAA"
     gene            complement(3241..4167)
                     /locus_tag="VFO53_06835"
     CDS             complement(3241..4167)
                     /locus_tag="VFO53_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET8876027.1"
                     /translation="MTETTERAANLPATVDATLELLEQQGYVAERRLATAAFLALKLK
                     RPLFLEGEAGVGKTEIAKVLAAGLTRPLVRLQCYEGLDTAAAVYEWNYPRQMIEIRLA
                     EAAGGVERSMLAHDIFTPRFLLRRPLLQALDPEEGGPPVLLIDELDRADEPFEAYLLE
                     VLSDYQVTIPELGTLRAATPPIVVITSNRTREIHDAIKRRCLYHWVDFPDAARELAIV
                     RRKCPAVSAALAREIVAFTQRLRTLDLFKSPGIAETLDWAEALVALDRITLDPQTVAD
                     TLGTLLKYQDDIGAITPDVAAKLVREAQSAPA"
     gene            complement(4207..>4553)
                     /locus_tag="VFO53_06840"
     CDS             complement(4207..>4553)
                     /locus_tag="VFO53_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HET8876028.1"
                     /translation="LGPDEIITAIEFPSQKRAGYEKFPNPASRFALVGVFVAQRDDGD
                     VRVAVTGAAPSVFRAAAIETALEKSFTADAAKATRVDSSGLNTDLHGSAEYRAHLVSI
                     MASRAVAACAAT"
BASE COUNT          791 a         1551 c         1476 g          735 t
ORIGIN      
        1 cgtctgcgtg gcgtcgaacg gcgccgtggc gagccgcggc tgcggcagcg gctcgaacgt
       61 cccgtgcagc agcgcgggac gaatccgata cagccacgag cgccggttcg cgtggcgcgg
      121 cacggtgaat gcggtgcccg aaagctgctc cgcataaagg ccgaacgccg gacgctgcgg
      181 cgaattgcgg ccgaccggca acgcgccggc gagcgcctcc gtcgcgaact cgctgccgaa
      241 gcccgagtta taccgtggcg tcatccgtcc gcgcgttgcg cttcgtcgac cacgctgcac
      301 gcgggcgtct gcgcgcgcgt gtccttgccc gcggccaccg atacgatccg cgacgcatcg
      361 accccgttct tgatcgccgt cacttcggcg aggatcgaga tggcgatctc cggcggcgtg
      421 cggctgccga tgtagaggcc gacgggcccg tgcagccgcg cgatctcggc gtccgacaag
      481 tcgaactcgc gcagccgctc gcggcgcttc tcgttgttcg cgcgcgagcc gatcgcgccg
      541 acgtagaacg ccggcgactt gagcgcttcc atcaatgcga gatcgtcgag cttcggatcg
      601 tgcgtcagcg ccacgacggc gcagcgctcg tcgagtgcga gtgcctgcac ggtgtcgtcg
      661 ggcatcgtcc gcacgagcgt gacgccgggg acgtcccacg tctccatgta ctcgtcgcgc
      721 ggatcgcaaa cggtcacctg gtaatcgagg ccgatcgcaa tctgcgcgag atacttcgac
      781 aactgcgacg cgccgatgac gagcatccga tagcgcgggc cgtggatcgt cgtcagcacg
      841 gcgccgtcga acgactggcc gtcgctcgcc cgcgcaggat gcagcgtcgc acgaccggtc
      901 gcaagatcga ggcgacgcgc cgtcaaatgt ccggcctcga tctcgcgcaa cagcgcccgg
      961 attccagact cacgcgtcag cggctcgagc acgagccgaa tcgttccgcc gcacggcaat
     1021 ccgaaccgcc gcgcctcgtc ggcgctgacg ccgtacgtga cgacatcaca gcgcgtctgc
     1081 tggaatccat cgtgacgcat gcgctcgatc aggtcgtcct cgatgcagcc gccggacacc
     1141 gatcctgccg caagcccgtc atcgcgaacg gcgagcatcg cgccatcggg acgcggcgag
     1201 ctgccccacg tcttcacgac ggtgacgagc agcgcacgat ggcctgcctc gagccattcg
     1261 tcgcattttt tcaacacatc gaggtcgacg ctgtccattg ccgtttctcc ggcgcgaaag
     1321 cttcagcgat agccgcgcac gtaaaggacg accagcacga cgacgatggc aacgatcgcc
     1381 gcgtagcgta tccagggaga cccacccgcc ggtgcgagcg gcgtcgacgt atcgtccact
     1441 gctgcgactt cgctcgacgc agcacttgtc gccgcggacg tcgctgcagg cgctgcgcgt
     1501 gcgacaaacc gcgcaaaaaa atcatcggcg agcttcgcgg ccgcgccatc gacgagccgc
     1561 gatccgatct gcgcgagctt gccaccgatc tgcgccttcg cgacgtacga catttccgtc
     1621 tgacgctcgt cgagcggctt cagtgtgacg cgcgcgtcgc cgcgcgcaaa acccgctgca
     1681 ccgccctggc catcgaattg cagcgtgtag ctttcgggtt cgttgatgtc ggtcatcgcc
     1741 atccggccat tgaagcgcgc ggagaccggt ccgacgcgtg cggccatcac gacgcgccac
     1801 gcgtgctcgc cgtcgggctc catcgattcg caaccgggta cgcaatcctt cagcacggcc
     1861 gggtcgttga gcgcattcca gacgacttcg cgcggcagcg ggacggtgcg ggtgttcgac
     1921 aattccatga cggactcgtg taagagcgtt gtcaggccgc ggcgggaagc ggcactcccc
     1981 gctcacgtcg aggccgtttc gccagcgcgt gcgtgagatc ggtcagcgtt tcgagattgt
     2041 gcaccggcag gaaatcgtcg acgtgcggca acagcgcgcg aatccccgcc ggccgcgctt
     2101 cgaaccccgc gtatcgcaac agcggattga gccacacgag ccgcgcgcac gatttcgaca
     2161 atcgttccgc ttcaaacgcg agaccatcgc cggcgtcggc gtcgagacca tcactgatga
     2221 gcagcacgac ggcattctgc ccgagcagcc tgcgcgacca taggcggttg aagtcaccga
     2281 ggcactggcc gatccgcgtg ccgccggccc agtcctcgat cgcccccgcg acatgcgcaa
     2341 gcgcgacgtc gacgtcgcgg tgacgcaggt gtcgcgtgat gttcgtgagc ctggtgccga
     2401 acgtcagcac gtgcacgcgg tgccgatcgt tcgtgatcgc atgcaggaaa tgcaagagca
     2461 tccgtgcgta acggtccatc gagcccgaga tgtcgcacag cacacaaagt ggcggcgtcc
     2521 gcattcgccg cgcgcgaaac tgcaacggcg tcagcgcacc accggcgcca acactgcgtc
     2581 gcagcgttgc tcgcaagtcg atccggtgtc cgcgtggcga cgcgatcgtc cgccgcacgg
     2641 caatttgggg caacggcagc ttcatcctgc gcaacagcgc cttcacctcg gccagctcgg
     2701 cgaccgacat cgtcgcgaaa tcctttttct gcagcacttc gcgcgccgag aacgagaatg
     2761 cggcatcgag attggactcg tcttcgcgcg attcgtggcg tggcggtggc ggcggcagca
     2821 gcgcttgcgc aaggcgcgcc ggcagttcgt cggcgtcgcc acctcgcccc tgcacctgcg
     2881 gcaacagcgc ggcgagcatt ttctcgagca gccggggatt gcgccagaag aggtcgaacg
     2941 cctgctcgaa cagcacgaga tgctcgcgct tcgacacgac gacggccgcg agtgcttcgc
     3001 ggaaatcgtc gcggttgccg atgccgaccg cctcgacggc cgcgagcgca tcgatcacct
     3061 tcgcaggtcc gaccggcagg ccggcgacgc gcaacattcg cacgaagtac acgacgttgt
     3121 cggcgaggtg gccagcagcc cttgcgagtg gcggagacaa cgtcgtaaag ccattggcgc
     3181 gcgcatcgct gccgctcaat ggacgttcgc tcggcgcggg catccgcgtg tcactcaccg
     3241 tcaggccggc gccgactgcg cctcgcgaac gagctttgcc gcaacatcgg gcgtgatcgc
     3301 gccgatgtcg tcctgatact tgaggagagt accgagcgtg tcggcgaccg tttgcggatc
     3361 gagcgtgatc cggtcgagcg caaccagcgc ctcggcccag tcgagcgtct cggcgatacc
     3421 cggcgacttg aaaagatcga gcgtgcgcag gcgttgcgtg aacgctacga tctcgcgcgc
     3481 cagcgcggcg gacaccgccg ggcatttgcg cctgacgatc gcgagctccc gcgcggcatc
     3541 cggaaagtcg acccagtgat agaggcagcg gcgcttgatc gcatcgtgga tctcgcgcgt
     3601 gcgattcgac gtgatgacga caatcggcgg cgtcgccgcg cgcagcgtgc cgagctccgg
     3661 gatcgtcacc tggtaatcgg acaaaacttc gagcaggtaa gcttcgaacg gctcgtcggc
     3721 gcggtcgagc tcgtcgatca gcaacaccgg cggcccgcct tcttccgggt cgagcgcctg
     3781 caacaagggc cgccgaagca ggaaacgcgg cgtgaaaata tcgtgcgcga gcatcgagcg
     3841 ctcgacgccg cccgcggcct cggcgagacg gatctcgatc atctgccgcg ggtagttcca
     3901 ttcgtagacg gcggcggccg tatcgaggcc ttcgtagcac tgcagccgga cgagcgggcg
     3961 tgtgaggccc gcggccagca ctttcgcgat ctcggtcttt cccacaccgg cctcgccctc
     4021 gaggaacagc gggcgcttga gcttcagtgc gaggaaggcc gccgtcgcga gcctgcgctc
     4081 ggccacatag ccctgctgct cgagaagctc gagcgtcgcg tcgacggttg caggaagatt
     4141 tgctgcgcgc tcggtagtct cagtcaaaaa aactcactcg gtctttcgaa cttcgatttt
     4201 gcgcactcag gttgcggcgc aggcagcgac cgcgcgagat gccatgatcg aaacgagatg
     4261 cgcgcggtat tcggcggacc catgcagatc ggtgttcaat ccgctcgaat cgacgcgcgt
     4321 tgccttggcc gcatccgcgg tgaagctctt ctcgagcgcc gtctcgatcg ccgcggcgcg
     4381 aaacaccgaa ggtgctgcgc ccgtcacggc cacgcgaaca tcaccatcat cccgctgcgc
     4441 gacgaacacg cccacgagcg caaaacgtga cgcaggattc ggaaacttct catagcccgc
     4501 gcgcttttgc gatgggaact cgatggccgt gatgatctcg tcgggtccca acg
//
LOCUS       DASSVX010000158         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1222875, whole genome shotgun sequence.
ACCESSION   DASSVX010000158 DASSVX010000000
VERSION     DASSVX010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222875"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VFO53_06845"
     CDS             <1..905
                     /locus_tag="VFO53_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876029.1"
                     /translation="YLDTTALAEQLDMTLAALRHSEETDQGPVGPINQQRALILDKAR
                     ASIAPNLNTDMRRDPRTPCVVAARVRIGLPRILHELAKPPGDTARPDSVVGTNEQIEV
                     YAVAGPVRATRPATEESDTLSASLASFSDPVWQVKDRSVAGLRIYAGSGVGQSLTLGA
                     LVAVRQSDVSGWVLGVVRRLNKLSTDEIEAGVSLIAERVVPVTLNAKREVKEDMGIVV
                     NGFDASLLGPKIEGLYLPPPSRPDKPLVVKTLIIPTHEYAEGRKIILTTGRSIYIVAM
                     RQLVEQRADWSWTAISIVEKLARC"
     gene            complement(940..1899)
                     /gene="tolA"
                     /locus_tag="VFO53_06850"
     CDS             complement(940..1899)
                     /gene="tolA"
                     /locus_tag="VFO53_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HET8876030.1"
                     /translation="MSTLAFTSDRPVNAPRARGRWTALMLALFVNLLFIAVLVFSVSW
                     QNRKPDAVTAELYAPPPVATPRPQPKVEPVPVPKVEPPPEPKPVPKPPAPVAEKPDTH
                     EADIALKARREAERRQQEQAERARIEQERERREAEAKAEAKKAEEKRQADARERQQQQ
                     QLEAMRAQADREARLRTQAERESQMRAQAQRESQMRAQAERESQLRAQADAEARGRAA
                     QAAAGAARNKSQADWIDKIRSRIRGNINLPPDIVGNPEAIFDVVQLPTGEIIDVKLRK
                     SSGVRAYDDAVQRGILKSSPLPKPVPADLFQRNLELRFRPNDQ"
     gene            complement(1906..2046)
                     /locus_tag="VFO53_06855"
     CDS             complement(1906..2046)
                     /locus_tag="VFO53_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET8876031.1"
                     /translation="MQAGRDQPVVIVADAGLRYEDVIGVLDRLQQGGVKRVGLLARPT
                     PS"
BASE COUNT          302 a          693 c          677 g          377 t
ORIGIN      
        1 gctatctcga tacgacggcg ctcgccgagc aactcgacat gacgctcgcg gcgttgcggc
       61 attccgaaga gaccgaccag ggtccggtcg gacccatcaa tcagcagcgc gcgctgattc
      121 tcgacaaggc gcgcgcatcg atcgcgccca atctcaatac cgacatgcgc cgcgatccgc
      181 gcacgccgtg cgtcgttgcg gcgcgcgtgc gaatcggatt gccgcgaatc ctccacgagt
      241 tggcgaagcc gccaggggac accgcgcgac cggattcggt cgtcggcacc aacgagcaga
      301 tcgaggtgta cgcggtcgcc ggtcccgtcc gcgcgacgcg tcctgccacc gaagagtcgg
      361 atacgctgtc cgcgagtctc gcgtcttttt ccgatcccgt gtggcaggtc aaggaccgca
      421 gcgttgcggg gctgcgcatt tacgcgggca gcggcgttgg ccagtcattg acgctcggcg
      481 cactcgtcgc ggttcgccag tccgatgttt cgggctgggt gctcggcgtc gttcgccggc
      541 tgaacaagct ttccactgat gaaatcgagg cgggcgtctc attgattgcc gagcgcgtcg
      601 ttccggtgac gctgaatgca aagcgcgagg tcaaggaaga catgggcatc gtcgtcaacg
      661 gcttcgatgc gtcgctgctc gggccgaaga tcgagggcct gtacctgccg ccgccatcgc
      721 gcccggacaa gccactcgtc gtcaagacgc tgatcattcc gacgcacgaa tacgcggagg
      781 gccggaagat catcctgacg accggccgct cgatctacat tgtcgcgatg cgtcagctcg
      841 tcgagcagcg cgccgattgg agctggaccg ccatctcgat cgtcgaaaaa ctcgcacgct
      901 gctagaacgc tggagcagcg ttgccgttcg caagccttgc tactgatcgt tcggccggaa
      961 gcgcaactcg agattgcgct ggaacaggtc ggccggcacg ggcttgggta gcggcgacga
     1021 tttcagaatt ccacgttgca cggcatcgtc gtaggcgcga acgccactcg acttgcgcaa
     1081 cttgacgtcg atgatctcgc ccgtcggcag ctgaacgacg tcgaagatcg cttcggggtt
     1141 gccgacgatg tccggtggca ggttgatgtt gccgcggatc cggctgcgaa tcttgtcgat
     1201 ccaatccgcc tgagacttgt tgcgcgcagc gcctgcagcc gcttgcgccg cgcgcccccg
     1261 cgcctcggca tcggcctgcg ctcgtagctg cgattcgcgc tccgcctgcg cgcgcatctg
     1321 cgactcccgt tgtgcttgtg cgcgcatctg ggactcgcgc tcggcctgcg tgcgcaagcg
     1381 cgcctcgcgg tccgcttgcg cgcgcatcgc ttcgagctgc tgctgttgct ggcgctcacg
     1441 cgcgtccgcc tggcgttttt cctccgcctt cttcgcttcg gccttcgctt cggcctcgcg
     1501 acgctcgcgc tcctgctcga tacgcgcccg ttccgcctgc tcctgctgcc ggcgctccgc
     1561 ttcgcgtctc gctttgagcg cgatgtcggc ttcgtgcgtg tccggtttct ccgcgacggg
     1621 cgccggtggt ttgggcacgg gcttgggctc gggcggcggc tcgaccttcg gtacgggaac
     1681 gggctcgacc ttcggctgcg gccgcggcgt ggcgaccggc ggcggggcat acagctcggc
     1741 ggtgacggcg tcgggcttgc ggttctgcca cgacaccgag aacacgagca cggcgatgaa
     1801 cagcaggttg acgaacagcg ccagcatcag cgccgtccac ctgccgcgcg cgcgcggcgc
     1861 gttgaccggc cgatcggacg tgaacgcgag cgtgctcatc aaattctacg aaggcgtggg
     1921 ccgcgcgagc aggccgacgc gcttgacgcc cccctgctgc agccgatcga ggacgccgat
     1981 gacgtcctcg taacgcaagc cggcgtcagc cacgatgacg accggctgat cgcgcccggc
     2041 ctgtatcgc
//
LOCUS       DASSVX010000159         3408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_806290, whole genome shotgun sequence.
ACCESSION   DASSVX010000159 DASSVX010000000
VERSION     DASSVX010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806290"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFO53_06860"
     CDS             complement(<1..501)
                     /locus_tag="VFO53_06860"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET8876032.1"
                     /translation="MSLPTFTFYAFAVILVFSALRVVTTRNPVHAALWLVLSFFTAAG
                     IWLLLQAEFLAIALVLVYVGAVMVLFLFVVMMLDVNFEALRQHFRSYLPIAAAVGALV
                     LFEMALVIVDSAIGRTTTAPPAPSGSNTRALGQLLYVDYVYPFEIAAVVLLVAIIAAI
                     ALTHRDR"
     gene            complement(626..1117)
                     /gene="nuoI"
                     /locus_tag="VFO53_06865"
     CDS             complement(626..1117)
                     /gene="nuoI"
                     /locus_tag="VFO53_06865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET8876033.1"
                     /translation="MINRIREFANTWFLLELFKGLALTGRYLFSRKVTVQFPEEKTPQ
                     SPRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIESEQREDGTRRTTRYDIDLTK
                     CIFCGFCEESCPVDSIVETRILEYHGERRGDLYYTKEMLLAVGDRYEPQIAADRAEDA
                     KYR"
     gene            complement(1123..2175)
                     /gene="nuoH"
                     /locus_tag="VFO53_06870"
     CDS             complement(1123..2175)
                     /gene="nuoH"
                     /locus_tag="VFO53_06870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET8876034.1"
                     /translation="MLAPLSNALGPWWLPVWTLVKVVVIAVPLIIGVAYLTYAERKVI
                     GWMQVRIGPNRVGPLGLLQPFADVLKMLFKEIIVPSGASRALFFIAPMLSLAPALAAW
                     AVIPFTPTLVLSDINAGLLYILAMSSIGVYGVILAGWASNSKYAFLGCLRSAAQIVSY
                     EIAMGFALVGVLMAANSLNLGDIVAGQEGGAAYWYVWPLLPLFVVYLVSGIAETNRHP
                     FDVAEGESEIVAGFHVEYSGISFALFFLAEYMNMILIATLTSIMFLGGWLSPFPILND
                     VGVFGAHPFGDGFPWLALKVAFVLLIFLWIRATFPRYRYDQIMRLGWKVFIPVTLVWI
                     VFLGAMMQTSYADMFH"
     gene            complement(2195..>3408)
                     /locus_tag="VFO53_06875"
     CDS             complement(2195..>3408)
                     /locus_tag="VFO53_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET8876035.1"
                     /translation="IGSFLRKDQPMLALRLRQAAKKGAQVSSLHSVDDNWLMPIAHKA
                     IVPPSQLPMMLAQIVVAAAQGAGKDAPPALRGIEPVAAADVIAASLATGERRAILLGN
                     YAMQHPDASQIVALAQALASITGATLGVCGEAANSVGGHLARALPQDDGLNAQAMLGG
                     DAGESRHAYVVLHAEPEFDCASPVTARAALEKADLVVVLSAFRTETRYADVLLPVAPF
                     TETSGTFVSCEGRVQSFHGVAQPPGDARPAWKVLRVLGTMLKLPGFALDTSEEVRDAV
                     LADAGDIGARLSNASRVEIALPARANGGIERVADVPIYFADPLVRRAPSLQASADAKP
                     PRARVNKTLIDQFGIDEGGQIKVRQGRGEAVLATQVDPGVPPGVVRIAAAHPSTCGLD
                     GLSGPVSVERA"
BASE COUNT          610 a         1147 c         1158 g          493 t
ORIGIN      
        1 gcgatcgcga tgcgtcagcg cgatcgccgc gatgatcgcc acgagcagga caacggcggc
       61 gatctcgaac ggatatacgt agtcgacgta cagcagttgc ccgagggcgc gcgtattgct
      121 gccgctcggc gccggcggcg ccgtcgtcgt gcggccgatc gcgctgtcga cgatcacgag
      181 cgccatctcg aagagcacga gcgccccgac ggcggcggcg atcggcaggt agctgcggaa
      241 gtgctggcgc agcgcctcga agttgacgtc gagcatcatc acgacgaaca ggaacagcac
      301 catcacggcc ccgacgtaga cgaggacgag cgcgatcgcg aggaactcgg cctgcagcag
      361 cagccagatg cccgccgcgg tgaagaacga cagcacgagc cacagcgcgg cgtgcaccgg
      421 gttgcgcgtg gtgaccacgc gcagcgcgga aaagaccagg atcaccgcga aggcgtagaa
      481 ggtgaacgtg ggcaggctca tcggatgcct agcggtcggt ctccgaatac caatggcgag
      541 acccgcagcg tgcgcgtcgg cctccccgac tcgcacgccg acgggccctt cgggccttgg
      601 ctcggcttcg cctctcccgt cgtgctcatc ggtacttcgc gtcctcggcg cggtcggcgg
      661 cgatctgcgg ctcgtaacga tcgccgacgg cgagcagcat ttccttcgtg tagtagagat
      721 cgccgcggcg ctcgccgtga tactcgagga tgcgcgtctc gacgatcgaa tcgaccgggc
      781 agctctcctc gcagaagccg cagaagatgc acttggtgag gtcgatgtcg tagcgcgtgg
      841 tgcgccgcgt gccgtcctcg cgctgctccg attcgatggt gatcgcgagc gccggacaca
      901 cggcttcgca cagcttgcat gcgatgcagc gctcctcgcc gttcgggtag cggcgcagcg
      961 cgtgcaggcc gcggaagcgc gggctctgcg gcgtcttctc ctcggggaac tgcacggtga
     1021 ccttgcgcga gaacaggtag cgtccggtca gcgcgagccc cttgaagagc tccagcagga
     1081 accacgtgtt ggcgaactcg cggatgcggt tgatcacgtt catcagtgga acatgtccgc
     1141 gtaggacgtc tgcatcatcg cgccgagaaa cacgatccag acgagcgtca ccggaatgaa
     1201 caccttccag ccgagccgca tgatctggtc gtagcgataa cggggaaacg tcgcgcggat
     1261 ccacaggaag atcagcagca cgaacgccac cttgagcgcg agccacggga agccgtcgcc
     1321 gaacggatgc gcgccgaaca cgccgacgtc gttcaggatc gggaacggcg acagccagcc
     1381 tccgaggaac atgatcgacg tcagcgtcgc gatcaggatc atgttcatgt attcggcgag
     1441 gaagaacagc gcgaacgaga tgcccgagta ttcgacgtgg aacccggcga cgatctccga
     1501 ttcgccttcg gcgacatcga acgggtgccg gttcgtttcg gcgatgcccg acacgaggta
     1561 gacgacgaac agcggcagga gcggccagac ataccagtat gcggcgccgc cctcctgtcc
     1621 ggcgacgatg tcgccgaggt tgaggctgtt ggccgccatc agcacgccga cgagtgcgaa
     1681 gcccatcgcg atctcgtagg agacgatttg cgcggccgag cgcaggcagc cgaggaacgc
     1741 atacttcgaa ttcgacgccc agcccgcgag gatgacgccg tagacgccga tcgagctcat
     1801 cgcgaggatg tacagcaggc ccgcgttgat gtcggacagc acgagcgtcg gcgtgaacgg
     1861 aatgaccgcc caggccgcga gcgcgggcgc gagcgagagc atcggcgcga tgaagaacag
     1921 cgcccggctc gcgcccgagg gcacgatgat ctccttgaac agcatcttca gcacgtcggc
     1981 gaacggctgc agcagcccga gcgggccgac gcggttgggg ccgatgcgca cctgcatcca
     2041 cccgatcacc ttgcgctcgg cgtacgtgag gtacgcgacg ccgatgatca gcggcaccgc
     2101 gatcacgacg accttgacga gcgtccacac cggcagccac cacgggccga gtgcgttcga
     2161 aagcggcgcg agcatgtcca tcagcgtctg cccgtcatgc ccgctcgacg ctgacgggtc
     2221 cggacagacc gtcgaggccg cacgtggacg gatgcgccgc ggcgatacgc acgacgcccg
     2281 gcggcacgcc gggatcgacc tgggtggcga gcaccgcttc gccgcggccc tgtcgcacct
     2341 tgatctgccc gccctcgtcg atgccgaact ggtcgatgag cgtcttgttg acccgcgccc
     2401 gcggcggctt cgcatccgcg ctcgcctgca gcgatggtgc gcggcgcacg agcggatcgg
     2461 caaagtagat cggcacgtcg gccacccgct cgatgccccc attggcgcgc gccggcagcg
     2521 caatctcgac gcgtgaggcg ttcgaaagac gcgcgccgat gtcgcccgcg tcggccagca
     2581 ccgcgtcgcg cacttcctcg gacgtgtcga gcgcaaagcc gggaagcttc agcatcgtgc
     2641 cgagcacccg caacaccttc cacgccgggc gcgcatcgcc cggcggctga gcgacgccgt
     2701 ggaacgactg cacgcggcct tcgcagctga cgaacgtgcc ggacgtttcg gtgaatgggg
     2761 ccaccggcag cagcacgtcg gcataccgcg tttcggtgcg aaacgcggac agcacgacca
     2821 cgaggtcggc cttttcgagt gcagcacgcg cggtgaccgg actcgcgcag tcgaactcgg
     2881 gctcggcgtg gagcacgacg tacgcgtgcc gcgactcacc ggcgtcgccg ccgagcatcg
     2941 cctgcgcatt caatccgtcg tcctgtggca gcgcgcgggc gaggtggccg cccacgctgt
     3001 tggccgcttc accgcatacg ccgagcgtcg cgccggtgat cgacgcaagc gcctgtgcga
     3061 gtgcgacgat ctgcgacgcg tcggggtgct gcatcgcgta attgccgagc aggatcgcgc
     3121 gccgctcgcc ggtggcaaga cttgcggcaa tgacatcggc ggccgccacc ggctcgatgc
     3181 cgcgcagcgc cggcggcgcg tccttgccgg cgccctgcgc agcggcgacg acgatctgtg
     3241 ccagcatcat cggcaactgc gacggcggca cgatcgcctt gtgcgcaatc ggcatcagcc
     3301 agttgtcgtc gaccgaatgc agcgacgaaa cctgcgcgcc cttcttcgcc gcctgccgca
     3361 gccgcagcgc gagcatcggc tggtccttgc gcaggaagct gccgatca
//
LOCUS       DASSVX010000160         3662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1039551, whole genome shotgun sequence.
ACCESSION   DASSVX010000160 DASSVX010000000
VERSION     DASSVX010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039551"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /gene="rpsI"
                     /locus_tag="VFO53_06880"
     CDS             <1..182
                     /gene="rpsI"
                     /locus_tag="VFO53_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET8876036.1"
                     /translation="ESGQAGAVRHGITRALIDYDAELKSALSKAGLVTRDAREVERKK
                     VGLHKARRRKQFSKR"
     gene            337..1368
                     /gene="argC"
                     /locus_tag="VFO53_06885"
     CDS             337..1368
                     /gene="argC"
                     /locus_tag="VFO53_06885"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET8876037.1"
                     /translation="MIRIGIVGGTGYTGVELLRLLAAHPQADVRAITSRKEAGTRVAQ
                     MFPSLRGHYDLAFCEPQDAKLADCNVVFFATPHGVAMGQARELLAAGVRIIDLAADFR
                     LSDPAVFERWYRMPHACPDLLAEAVYGLPEVNREAIRAARIVANPGCYPTSVQLGFLP
                     LLEAGLVDTRYLIADCKSGVSGAGRKAELSLAFAEASDNFAAYGVAGHRHLPEIVQGM
                     NRASREPVRLTFTPHLTPIIRGILATLYAPLRDADADVQGIYEKRYAGEPFVDVMPPG
                     SLPDTRSVRASNTLRIAVHRPPDSDLAVVIAVLDNLVKGAAGQAIQNMNLMFGLPETS
                     GLEAPPVMP"
     gene            1515..1865
                     /gene="erpA"
                     /locus_tag="VFO53_06890"
     CDS             1515..1865
                     /gene="erpA"
                     /locus_tag="VFO53_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET8876038.1"
                     /translation="MNAMNEMPSPLVFTDAAAAKVKNLIDEEGNPDLKLRVFVSGGGC
                     SGFQYGFTFDEVVNEDDTAMNKGGVTLLIDPMSYQYLVGAEIDYQEGLEGAQFVIKNP
                     NAQSTCGCGSSFSA"
     gene            complement(1938..3047)
                     /locus_tag="VFO53_06895"
     CDS             complement(1938..3047)
                     /locus_tag="VFO53_06895"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HET8876039.1"
                     /translation="MARELFVGVMSGTSLDGVDAVVADFAVPGKACATLGAAHIGFPS
                     LLRDELLALQQPGSNELSRAGAAANMLAELYSDAIARALTAGKVDRGDVVAAGVHGQT
                     LRHRPDRGFTVQLNNPARVAEKAGVRVVADFRSRDVAAGGQGAPLVPAFHAALFARPD
                     RHRVIVNIGGIANITDLAPDGTVHGFDTGPGNVLSDLWCARNRGMTFDAQGAWAGAGK
                     PNPALLDVLLADPFFSAAPPKSTHRDKFNLQWFEERLAAAHIDDEAENIQATLVALTA
                     RTIADAIRAYAPTAEEILACGGGANNATLMRYLAAEVAPRRLSTTADEGVPVEQVEAL
                     AFAWLAREALAGRPANLPEVTGARGKRILGAIYPR"
     gene            complement(3104..>3662)
                     /locus_tag="VFO53_06900"
     CDS             complement(3104..>3662)
                     /locus_tag="VFO53_06900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET8876040.1"
                     /translation="TEGYYNEDGRNSRNAFLRSPMEFSRVTSGFTLARLHPVLHTMRA
                     HKGTDFAAPIGTPVRATADGVVTFVGQQTGYGNVIMLKHDGRYSTVYAHLSRFAPETR
                     DGARVHQGETIGYVGQTGWATGPHLHYELRVDGEPRDPLTVALPMATPVTDQQRPAFA
                     AAIAPLAGELAVVRELPVSRLVAAN"
BASE COUNT          597 a         1220 c         1268 g          577 t
ORIGIN      
        1 gcgagtcggg acaggcgggc gcggtccgcc acggcatcac ccgcgcgctg atcgactacg
       61 atgccgagct caagtcggcg ctgtcgaagg cgggtctcgt gacgcgcgac gcgcgcgagg
      121 tcgagcgcaa gaaggtcggc ctgcacaagg cgcgcaggcg caagcagttc tcgaagcgct
      181 gatcgggctt cccgcgcgcc gcacgcacgt cgcgctgcac cggcaagggc cgtctcgcag
      241 gagacggcct ttttcgtcga ccgaggcggc ggaccgaagt gcggagtgcc gggcgacgcg
      301 aatgctaaga tcacggcgta accaaggcta cgcgacatga tcaggatcgg catcgtcgga
      361 ggtaccggct atacgggcgt cgaattgctg cggctcctcg ccgcacaccc gcaagccgac
      421 gtgcgcgcga tcacgtcgcg caaggaagcc ggtacgcgcg tcgcgcagat gttcccgagc
      481 ctgcgcggcc attacgatct cgcgttctgc gagccgcagg atgcgaagct cgccgactgc
      541 aacgtggtgt tcttcgcgac gccgcacggc gtggcgatgg gacaggcgcg cgaacttctt
      601 gccgccgggg tgcgcatcat cgatcttgcc gccgatttcc ggctgtcgga tcccgcggtg
      661 ttcgagcgct ggtacaggat gccgcatgcg tgccccgatc ttctggccga ggccgtctac
      721 ggcctgccgg aagtcaaccg cgaagcgatc cgtgccgcgc gcatcgtcgc caatcccggc
      781 tgctatccca cgagcgtgca actcggcttc ctgccgctgc tcgaagccgg cctcgtcgac
      841 acgcgctacc tgatcgccga ctgcaagtcg ggggtgtcgg gcgccggccg gaaggcggag
      901 ctttcgctcg cgttcgccga ggcgtcggat aatttcgccg cgtatggcgt ggccgggcat
      961 cggcacctgc ccgaaatcgt gcagggcatg aaccgcgcga gccgcgagcc ggtacggctc
     1021 acgttcacgc cgcacctcac tccgatcatc cgtggcatcc tcgcgacgct ttacgcgccg
     1081 ttgcgcgacg ccgatgccga cgtgcaggga atctacgaaa agcgttacgc cggcgagccg
     1141 ttcgtcgacg tcatgccgcc gggctcgctg cccgacacgc gcagcgtgcg tgcgtcgaac
     1201 acgctgcgca tcgcggtgca ccgcccgccc gattcggacc tcgccgtcgt catcgcggtg
     1261 ctcgacaatc tcgtcaaggg cgccgcaggg caggcgatcc agaacatgaa tctcatgttc
     1321 gggctgcccg aaaccagcgg gctcgaggcg cccccggtca tgccgtgacc gcaccccgat
     1381 gcgcggggct ttgattaatg cggtgcacgc caccatattc ggctgatcgc cgcaccgacg
     1441 cgcctggcac gcttttgacg acccgccccg cgcggtcgat aatgtcggaa tagaggaaac
     1501 acgggagcac gtaaatgaat gcgatgaacg agatgccgag cccgctggtg ttcaccgacg
     1561 ccgcggccgc caaggtgaag aacctgatcg acgaggaagg caatcccgac ctcaagctgc
     1621 gcgtgttcgt ttccggcggc ggctgctcgg gcttccagta cggcttcacg ttcgacgagg
     1681 tggtgaacga ggacgacacc gcgatgaaca agggcggcgt cacgctcctg atcgacccga
     1741 tgagctacca gtacctggtc ggcgccgaga tcgactacca ggaggggctg gagggcgcgc
     1801 agttcgtgat caagaatccg aacgcgcagt cgacgtgcgg ttgcgggtcg agcttttcgg
     1861 cctgacgcgt cgcatcatgc atcggaaagg ggcccgcggg ccccttttgc gtttctggcg
     1921 cagggacgcg accttcgtca gcgcggatag attgcgccca ggatgcgctt gcccctggcg
     1981 ccggtgactt ccggaagatt ggccggccgc ccggccagcg cttcgcgtgc gagccatgcg
     2041 aaggcgagcg cctccacctg ctccaccgga acgccctcgt ccgccgtcgt cgaaagcctg
     2101 cgcggcgcga cctccgccgc aaggtagcgc atcagcgtcg cgttgttggc gccgccgccg
     2161 caggcgagaa tttcctccgc cgtgggcgca tacgcgcgga tggcatcggc gatcgtgcgc
     2221 gcggtgagcg cgaccagcgt tgcctggatg ttctcggcct cgtcgtcgat atgcgcggcc
     2281 gcgaggcgct cctcgaacca ctgcaggttg aatttgtcgc gatgcgtgct cttcgggggt
     2341 gcggcgctga agaacggatc ggcgagcagc acgtcgagca gtgccggatt cggctttccg
     2401 gcccccgccc acgcgccctg cgcgtcgaac gtcatgccgc ggttgcgtgc gcaccacaga
     2461 tcggaaagca cgttgccggg gccggtgtcg aagccgtgca ccgttccatc gggcgcgaga
     2521 tcggtgatgt tggcgatgcc gccgatgttg acgatcacgc ggtgccggtc gggccgggcg
     2581 aagagcgccg cgtggaacgc ggggacgagc ggcgcgccct ggccgcccgc cgccacgtcg
     2641 cggctgcgaa aatcggcaac gacgcgcacg ccagccttct ccgccacgcg cgccggattg
     2701 ttgagttgca cggtaaagcc gcgatcgggg cgatggcgca gtgtttgtcc gtgaaccccc
     2761 gcggcaacga cgtcgccgcg atcgaccttg cccgccgtca gcgcgcgcgc gatcgcatcg
     2821 gaatagagct ccgccagcat gttcgccgcg gcgccggcgc gcgaaagctc gttactgccc
     2881 ggctgctgca gcgcaagcag ctcgtcgcgc aacagcgacg gaaagccgat atgcgccgcg
     2941 ccgagcgttg cgcacgcctt tccgggcacc gcgaagtcgg cgacgacggc atcgacaccg
     3001 tcgaggctcg ttccggacat cacgccgacg aagagttcgc gagccacgga atcggcaaag
     3061 gccagggaga ccgcgctggg tgccccgacg cagcttagtg aaactagttt gcggcgacga
     3121 gccggctgac gggcaactcg cgcacgacgg cgagctcacc ggcgagcggc gcaatggccg
     3181 ccgcgaatgc cggacgctgc tgatcggtaa cgggggtcgc catcggcaat gcgaccgtga
     3241 gcgggtcgcg aggttcgccg tcgacgcgca attcgtagtg caggtgcgga ccggtcgccc
     3301 agccggtctg tccgacgtaa ccgatcgtct cgccttggtg gacgcgcgcg ccgtcgcgcg
     3361 tttcgggagc gaagcgggac aggtgcgcgt agacggtcga gtagcgcccg tcgtgcttca
     3421 gcatgatcac gttgccatag ccggtctgct gtccgacgaa cgtgacgaca ccgtcggcgg
     3481 ttgcacgcac cggcgtgccg atcggggcgg cgaagtcggt gcccttgtgc gcgcgcatcg
     3541 tatgcaggac cggatgcagc cgagcgagcg tgaagcccga cgtgacgcgc gagaattcca
     3601 tcggcgagcg caggaatgcg ttgcgcgaat tgcggccatc ctcgttgtaa tagccttccg
     3661 tg
//
LOCUS       DASSVX010000161         5681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1322927, whole genome shotgun sequence.
ACCESSION   DASSVX010000161 DASSVX010000000
VERSION     DASSVX010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322927"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..805)
                     /locus_tag="VFO53_06905"
     CDS             complement(62..805)
                     /locus_tag="VFO53_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HET8876041.1"
                     /translation="MPLQLVEPKRLYRQIADQLAQLIARGEYPAGTRLPAERELAASF
                     GVSRASVREAIISLEMTGLVEVRVGTGIFVTPQPETVVRPRGDAGPGPFELLAARKLV
                     EGEIAAQAARNATPAHAEALRRSVDRLEACVNDFVAREAEDRNFHLLLAKATGNGSLE
                     LVVEGLWNQRAELWGRLQRHFHTNDLALRTIRDHAAIAAAVDAGNADAARSAMHRHLS
                     RVTREFQRGVDNKAASKSTGTSPFTGGRK"
     gene            968..2014
                     /locus_tag="VFO53_06910"
     CDS             968..2014
                     /locus_tag="VFO53_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8876042.1"
                     /translation="MNRASRHRIAIVGLGMAAAPHARSLRDLAGRVDVVAAFSPTKAR
                     RDAFAAQFDFPVTGDIDAIVGDASIDCALILTPPRAHLPLVERFAAAGKHILLEKPLE
                     ADTWRAEAVVDVCARNGVTLGVVLQHRFRPGARALAEYITGGGLGEIATASVAVPWWR
                     PQSYYDEPGRGTLARDGGGVLLTQAIHTLDLFLSLVPPLAEVGAYACTTPLHRMETED
                     LACAALRFANGALGTLNATTASRPGFPERIDIVGTRGTATLVAGSLEVHFLDGHVESA
                     GEAAAFGGGADPMAFAHDAHRALLAEFLDAVDAGRAPPNDGHAALAVHDLIDAVLASA
                     RTHAFVPVLARPRP"
     gene            2011..3219
                     /gene="manD"
                     /locus_tag="VFO53_06915"
     CDS             2011..3219
                     /gene="manD"
                     /locus_tag="VFO53_06915"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HET8876043.1"
                     /translation="MKITAARVIVTCPGRNFVTLKIETDEGVHGVGDATLNGRELAVA
                     AYLTEHVIPVLIGRDPARIEDAWQYLYRGAYWRRGPVTMSAIAAVDTALWDIKGKVAG
                     LPLYELLGGASRDAVMVYGHANGKDIAETADEVARYIDLGYKAIRAQCGIPGLPSTYG
                     VAKDKLYYEPADAAIPTENVWSTTKYLDHVPKLFDTLRARFGFEPHLLHDVHHRLTPI
                     EAGRLGRALEPYRLFWMEDPTPAENQEAFRLIRQHTVTPIAVGEVFNSIFDCQALIGG
                     QLIDYIRTTVVHAGGITHLRRIAHLAELYQVRTGSHGATDLSPVCMGAALHFDTWVPN
                     FGIQEYMRHTPETDAVFPHAYRFDNGYLHPGGAPGHGVDIDETLAARYPYRPSALPVN
                     RLEDGTMWNW"
     gene            complement(3236..3430)
                     /locus_tag="VFO53_06920"
     CDS             complement(3236..3430)
                     /locus_tag="VFO53_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876044.1"
                     /translation="MSALAWAGRLLGIVGIVVCAVAGMARVTGHYVLGGFPAITLFQG
                     GTTALVAACFCLLWALAARR"
     gene            complement(3444..3998)
                     /gene="moaC"
                     /locus_tag="VFO53_06925"
     CDS             complement(3444..3998)
                     /gene="moaC"
                     /locus_tag="VFO53_06925"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET8876045.1"
                     /translation="MPRSRNKTSDNTPGADRTATGDVRTSGAHFTHFDASGQAQMVDV
                     GDKDVTKRVARAAGRIAMNASTLAMVRAGTVKKGDVLGVARIAAIQAAKRTSDLIPLA
                     HPLMLTRVSVVFDYEATGVAIEVTVETHERTGVEMEALTAVSAGLLTIYDMCKAADRA
                     MRIGDVHLLEKSGGRSGHYRVGEG"
     gene            4146..>5681
                     /locus_tag="VFO53_06930"
     CDS             4146..>5681
                     /locus_tag="VFO53_06930"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET8876046.1"
                     /translation="MRGTAAPPDASGALPEYGAAAPPPAAPIALPQAYPQRLPDLGDV
                     SQTDFSPAQERKLGETIVRELRANGGYINDPEVNDYLNELGHRLTAASTDVNQDFEFF
                     GVPDSQINAFALPGGYIGVNTGLILLTQTESELASVLAHEISHVTQHHMARALSAQKD
                     SMLMSLAGLALAILAARSGSGSSGDAAAGAIAATQALTLQHQINFTRENEYEADRLGF
                     QRLDAAGFDVTAMATFMQRLQTAMRFVEGSVPTYLRDHPVTYERIAEAQARAYGHPYR
                     QVVDSLDFHLVRALIQSYQGEPREAVKWFDDAIAEHKYNNAVAAQYGLVASLLRTTDF
                     ARAKRELAKLEKMTPEHPMIDAIAGHVLLESGDVDGAISRFGAAVARYPNKMQLVYDY
                     PDALLKAGRNADAAKFVEQQLTRFPSDGPLQLLAARAYAAQNKQMLAHRHQGEYYAWQ
                     GNLKGAVDQLEIAAKAKDGDFYQASVVDTRLKALRREVADQSKAAFGRQGFDVQGATD
                     ARLP"
BASE COUNT          943 a         2018 c         1832 g          888 t
ORIGIN      
        1 accgcgaccg ccgtgattgc ccgcgccgcc cggcgccttc cctgcgaatg cacgctgcgt
       61 ttcatttacg tcctcctgtg aatgggctcg tgcccgtcga tttcgacgcc gccttgttat
      121 cgacgcctcg ctgaaactcg cgcgtcaccc gcgacaggtg ccgatgcatc gccgaacgtg
      181 cggcgtcggc attccccgcg tcgaccgccg cggcgattgc cgcatgatcc cggatcgtgc
      241 gcaacgcaag atcgttcgta tgaaagtgtc gctgcaagcg cccccagagc tcggcgcgct
      301 gattccacag cccttcgacg acgagctcga gcgagccgtt gccggtggcc ttggcgagca
      361 gcagatgaaa gttgcggtcc tcggcctcgc gcgccacgaa atcgttcacg cacgcctcga
      421 gccgatcgac gcttcggcgc agcgcctcgg catgcgccgg cgtcgcattg cgcgcggcct
      481 gcgcggcgat ctcgccctcg acgagcttgc gcgccgcgag caattcgaac ggacccggcc
      541 ccgcatcacc gcggggccgc acaacggttt ccggctgcgg cgtgacgaaa atgccggtac
      601 cgacgcgcac ctcgaccagg ccggtcattt cgagcgagat gatcgcctcg cgcaccgatg
      661 cgcggctcac gccgaacgac gcggcgagct cgcgttcggc cggaaggcgc gtgccggccg
      721 ggtactcgcc acgggcgata agctgcgcga gctggtcggc aatctggcga tacaggcgct
      781 tcggctcgac gagctgcagt ggcaacggat tgcgctccgg aaaggggtga gccaggcgtg
      841 acaaaaggtc cggccatgcc ctgcactggc atgcagggat gctctcgacg catggtagcg
      901 tcgccgttat actgtggtca agtggctgag ccaccggacc atcgtttgga catcgacgcc
      961 gcacgccgtg aaccgcgcct cccgccaccg cattgccatc gtcgggctcg gcatggccgc
     1021 agcgccccat gcgcgaagct tgcgcgatct cgccggccgt gtcgacgtcg tcgccgcctt
     1081 cagcccgacg aaggcgaggc gcgacgcctt cgcggcgcaa ttcgactttc cggtcaccgg
     1141 cgacatcgac gcaatcgtcg gcgatgcgtc gatcgattgc gcactgatcc tgacgccgcc
     1201 gcgcgcgcat ctgccgctcg tcgagcgttt cgcggccgcc ggcaagcaca tcctgctcga
     1261 aaagccgctc gaagccgata cctggcgtgc cgaggccgtc gtcgacgtct gcgcgcgcaa
     1321 cggcgtcacc ctcggtgtcg tgctgcagca tcgcttccgg ccgggcgcgc gcgcgctggc
     1381 cgagtacatc accggcggag gtctcgggga aatcgcgacc gcgtcggtcg cggtgccgtg
     1441 gtggcgtccg cagtcgtact acgatgagcc gggacgcgga acgctcgcgc gcgacggtgg
     1501 cggggttctg ctcacgcagg ccatccacac gctcgacctg tttctgtcgc tcgttccacc
     1561 gctcgccgaa gtcggcgcgt atgcgtgcac caccccgctg caccgcatgg aaacggaaga
     1621 cctcgcgtgc gcggcactcc gcttcgccaa cggcgccctc gggacgctca acgcgacgac
     1681 ggcgagccgg ccgggcttcc ccgagcgcat tgacatcgtc ggcacgcgcg gcacggcaac
     1741 gcttgtcgcg ggaagcctgg aagtgcattt cctcgatggc catgtggaga gcgcaggcga
     1801 ggccgccgcg ttcggtggcg gtgccgatcc gatggcgttc gcccacgatg cgcaccgcgc
     1861 actcctcgcc gaattcctcg acgccgtcga cgccggccgc gcaccaccca acgacggcca
     1921 cgccgcgctc gccgttcacg atctgatcga cgccgtgctc gcttcggcgc gcacgcacgc
     1981 cttcgttccc gttctcgcac gcccacgacc atgaaaatca ccgccgcgcg cgtcatcgtc
     2041 acctgtcccg gacgcaattt cgtcacgctc aagatcgaga ccgacgaggg cgttcacggc
     2101 gtcggcgacg ccacgctgaa cggccgcgag ctcgccgtcg ccgcgtatct gaccgagcac
     2161 gtgatcccgg tgctgatcgg ccgcgacccg gcgcggatcg aggacgcgtg gcaatacctc
     2221 tatcgcggcg cgtactggcg gcgcggaccg gtgacgatga gcgccatcgc ggccgtcgac
     2281 accgcgctgt gggacatcaa gggcaaggtt gccggactgc cgctgtacga gcttctcggc
     2341 ggcgcgagcc gcgacgccgt gatggtgtac gggcacgcga acggcaaaga catcgccgaa
     2401 accgccgacg aggtcgcgcg ctacatcgat ctcggctaca aggccattcg cgcgcaatgc
     2461 ggcattcccg gactgccgtc gacgtatggc gtcgcgaagg acaagctgta ctacgagccg
     2521 gccgacgccg cgatccccac cgagaacgtc tggtcgacga cgaaatatct cgatcacgtg
     2581 ccgaagcttt tcgacacgct gcgcgcacgc ttcggcttcg agccgcacct gctgcacgac
     2641 gtgcatcacc ggctgacgcc gatcgaagcc ggacggctcg gacgcgcgct cgagccgtat
     2701 cggctgttct ggatggagga cccgacgccg gccgagaatc aggaggcgtt tcgcctgatt
     2761 cggcagcata cggtgacgcc gatcgcggtc ggcgaggtgt tcaacagcat tttcgactgc
     2821 caggcgctga tcggcgggca gttgatcgac tacatccgca cgaccgtcgt gcacgcgggc
     2881 ggcatcacgc atctgcgccg catcgcgcat ctcgccgagc tctaccaggt gcgcaccggg
     2941 tcgcacggcg caaccgacct gtcaccggtc tgcatgggtg ccgcgctgca tttcgacacg
     3001 tgggtcccga acttcggcat ccaggaatac atgcgccata cgcccgaaac cgatgcggtg
     3061 ttcccgcatg cataccgctt cgacaacggc tacctgcatc ccggcggcgc acccggtcat
     3121 ggcgtcgaca tcgacgagac gcttgccgcg cgctacccgt atcggccgag cgccctcccg
     3181 gtcaaccgcc tggaagacgg gacgatgtgg aactggtaag cggctgagct tgcggtcagc
     3241 ggcgcgcggc cagcgcccac aacaggcaga agcaggctgc caccaatgcc gtcgttccgc
     3301 cctggaacag cgtgatcgcc ggaaagccgc caagcacgta atgcccggtc acgcgcgcca
     3361 ttcctgcgac cgcgcacacg acgatgccaa cgattcccag cagccgtccg gcccatgcca
     3421 gcgcgctcat cgttccctcc gctttatccc tcacccacgc ggtaatggcc cgagcgtccg
     3481 ccgctcttct cgagcaggtg cacgtcgccg atgcgcatcg cgcgatcggc cgccttgcac
     3541 atgtcgtaga tcgtcagcag gcccgccgac accgcggtga gcgcttccat ttcgacaccg
     3601 gtgcgctcgt gcgtctcgac ggtgacttcg atcgcgacgc ccgttgcttc gtagtcgaac
     3661 acgacgctga cgcgggtcag catcagcgga tgcgcgagcg ggatgaggtc ggacgtccgc
     3721 ttcgcggcct ggatggcggc gatgcgcgcc acgccgagca cgtcgccctt cttcacggtt
     3781 cccgcgcgca ccatggcaag cgtcgacgcg ttcatcgcga tgcggccggc cgcgcgcgca
     3841 acacgcttcg taacatcctt gtcgccgacg tcgaccatct gcgcctgtcc cgacgcgtcg
     3901 aagtgggtaa aatgcgcacc tgaggtccga acgtcccccg tcgccgtgcg atcggcgcca
     3961 ggcgtgttgt cgctggtctt gttgcgtgag cgcggcatcg gtgggacggc agtcgcgtga
     4021 actccctcgc gagggcggca tcttagcacc acgcaaattc gtttcgaccc gtgcccgacc
     4081 cccgatcatt gcgtaatcga cgcgtcctcg ccgccatcag tgcccttgcg ctcgcatgcg
     4141 cgccgatgcg cggaacggct gcgccgccgg acgcatccgg cgccctgccg gagtacggcg
     4201 cggccgcgcc gccaccagca gcgcccattg cgctcccgca ggcttatccg cagcgcctgc
     4261 ccgatctcgg cgacgtctcg caaaccgact tctcgccggc ccaggagcgc aagctgggcg
     4321 aaacgatcgt gcgcgagctt cgcgcgaacg gcggctacat caacgacccg gaggtcaacg
     4381 actacctgaa cgagctcggg caccggctga cggcggcatc gaccgacgtc aaccaggact
     4441 tcgagttctt cggcgtgccc gattcgcaga tcaacgcctt cgcgttgccg ggcggctaca
     4501 tcggtgtcaa caccggactg atcctgctca cgcagaccga gtccgagctc gcgagcgtgc
     4561 tcgcgcatga aatctcgcac gtcacgcagc accacatggc gcgcgcgctc tcggcgcaga
     4621 aggattcgat gctgatgtcg ctggcggggc tggcactcgc catccttgcg gcgcgcagcg
     4681 gcagcgggtc gagcggcgat gccgcggcgg gcgcgatcgc ggccacgcag gcgctgacgc
     4741 tgcagcacca gatcaacttc acgcgcgaga acgagtacga ggccgaccgg ctcggtttcc
     4801 agcgcctcga tgccgccggc ttcgacgtga cggcgatggc gacgttcatg cagcggctgc
     4861 aaaccgcgat gcgcttcgtc gagggcagcg tgccgacgta cctgcgcgac cacccggtca
     4921 cgtacgaacg gatcgccgag gcacaggcgc gcgcgtacgg gcacccgtac cggcaggtcg
     4981 tcgactcgct cgattttcac ctcgtgcgcg cgctcatcca gagctaccag ggcgagccgc
     5041 gcgaggccgt caaatggttc gacgatgcca tcgccgagca caagtacaac aacgcggtcg
     5101 ccgcgcaata cggcctcgtg gcatcgctgt tgcgcaccac cgatttcgcc cgcgcgaaaa
     5161 gggagctcgc caagctcgag aagatgaccc ccgagcatcc gatgatcgac gccattgccg
     5221 ggcacgtgct gctcgagtcg ggcgatgtcg acggtgcgat cagccgcttc ggcgcagcag
     5281 ttgcgcgcta cccgaacaag atgcagctcg tctacgacta tcccgacgcg ctgctcaagg
     5341 ccgggcgcaa cgccgacgca gcgaagttcg tcgagcagca gttgacgcgc tttcccagcg
     5401 acgggccatt gcagcttctc gccgcgcgcg cgtacgccgc gcagaacaag cagatgctcg
     5461 cgcaccggca tcagggcgag tactacgcgt ggcaaggcaa cctgaaaggc gcggtcgacc
     5521 agctcgagat cgcggcgaag gccaaggatg gcgatttcta ccaggcgtcg gtcgtcgaca
     5581 cgcggctgaa ggcattgcgc cgcgaagtgg ccgatcaaag caaagcggca ttcggacgcc
     5641 aggggttcga cgtgcagggt gcaaccgatg cgcgcctgcc g
//
LOCUS       DASSVX010000162         3032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_756085, whole genome shotgun sequence.
ACCESSION   DASSVX010000162 DASSVX010000000
VERSION     DASSVX010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756085"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="VFO53_06935"
     CDS             <1..1382
                     /locus_tag="VFO53_06935"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET8876047.1"
                     /translation="NVDGHDVDAVSAAIDAGKAQTSRPTLICCKTIIGKGAPTKAGTA
                     DTHGAALGEREVAATRAALGWPSAAFVVPQAIYDAWNAMPRGERAEREWSASFSDYAA
                     EHPALAREFGRRIAGRLPDDFAGTAAKLVETQAARAESIATRKASQQAIEALAKVLPE
                     MLGGSADLTGSVFTNWSGSVAVTAQHPGNYVNYGVREFAMAAIANGLALHGGFIPYVG
                     TFLTFSDYMRNAVRMAALMKAKSIFVFTHDSIGLGEDGPTHQSIEHAASLRLIPQLDV
                     WRPCDTVESAVAWSSALARRGPSALLFTRQSVAFVARDASVQANIARGGYVLADFGDP
                     DVRAVIIATGSEVTLALGARERLAREGIGVRVVSMPCTGQFDRQDETYRRSVLPPGVP
                     RLAIEAGATDGWRKYVGAADDLHAAVIGIDRFGESAPANALFPFFGFTVDNVVEAVRR
                     ISGRQRPAP"
     gene            1379..1945
                     /locus_tag="VFO53_06940"
     CDS             1379..1945
                     /locus_tag="VFO53_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8876048.1"
                     /translation="MTLTIRAASVDDAQPIARIRIDCWRSTYRGLIPDAYLDAMEIDA
                     SAALWTRVLATSTDKASVFVADDDGEIVGFAAGNMLEEPRHGLDAELSAAYVRRDHQR
                     AGIGRRLVDAVARAQRAQGARGLIVWVIAGNKGARAFYEGLGATLLIEQPFEWDGMPL
                     IEAGYGYSDIDALIRACETGGAHGSTVH"
     gene            2040..>3032
                     /gene="gap"
                     /locus_tag="VFO53_06945"
     CDS             2040..>3032
                     /gene="gap"
                     /locus_tag="VFO53_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET8876049.1"
                     /translation="MTIKVAINGYGRIGRNILRAHYEDGKKHDLAIVAINDLGSPETN
                     AHLTRHDTAHGHFPGRIDVEGDAMVVNGDRIRVFAERNPAQLPWGSLGVDVVLECTGL
                     FTTKEKASAHLKAGARKVIISAPGGKDVDATIVYGVNHQKLRASDTVISNASCTTNCL
                     APLVKPLHERIGIVNGLMTTVHAYTNDQVLTDVYHEDLRRARSATMSMIPTKTGAASA
                     LGLVLPEMAGRLDGYSIRVPTINVSLVDLSFVAARDTSVEEVNRVMKEASESGPLSGG
                     ILRYNIEPLVSVDFNHDPASSTFDATLTKVSGRLVKACSWYDNEWGFSNRMLDVT"
BASE COUNT          525 a         1024 c         1026 g          457 t
ORIGIN      
        1 cgaacgtcga cggccacgac gtcgacgcgg tcagcgcggc aatcgacgcc ggcaaggcgc
       61 agacatcgcg gcccacgctg atctgctgca agacgatcat cggcaagggt gcgccgacga
      121 aggcgggcac cgccgacacg cacggcgcgg cactcggcga gcgcgaagtc gcggcaacgc
      181 gtgcagcact cggctggccc tccgcggcgt tcgtcgtgcc gcaggccatc tacgacgcgt
      241 ggaatgcgat gccgcgcggc gaacgcgccg aacgcgagtg gagcgcatcc ttttcggact
      301 atgctgccga gcaccctgcg cttgcgcgcg agttcgggcg gcgcattgcg ggcaggctgc
      361 ccgacgactt tgcaggcacc gcggcgaagc tcgtcgaaac gcaggcggcc cgggccgaat
      421 cgatcgcgac gcgcaaggcg tcgcagcagg cgatcgaggc cctcgcgaag gtgctgcccg
      481 aaatgctcgg cggctcggcc gatctgaccg gctccgtctt cacgaactgg tcgggcagcg
      541 tcgccgtcac tgcgcagcat ccgggcaatt acgtgaacta cggcgtgcgc gagttcgcca
      601 tggcggcgat cgcgaacggg ctcgcactcc atggcggatt catcccgtac gtcggcacgt
      661 tcctgacgtt ctccgactac atgcgcaacg cggtgcgcat ggccgcgctg atgaaggcga
      721 agtcgatctt cgtgttcacg cacgattcga tcggcctcgg cgaggacggg cccacgcacc
      781 agtcgatcga gcacgcggcc agcctgcgcc tcattccgca gctcgacgtc tggcggccgt
      841 gcgacacggt ggaaagcgcc gttgcgtggt cgtcggcgct cgcgcgtcgc ggtccgagcg
      901 cactgctctt cacgcggcag agcgtggcgt tcgtcgcgcg cgacgcgagt gtccaggcga
      961 acatcgcgcg cggcggctac gtgctcgccg acttcggtga tcccgacgtg cgcgcggtga
     1021 tcatcgccac cggctccgaa gtgacgctcg cgctcggcgc acgcgagcgc ctcgcacgcg
     1081 agggcatcgg cgtgcgtgtg gtgtcgatgc cgtgcaccgg gcagttcgac cgccaggacg
     1141 aaacctatcg ccgcagcgtg ctgccgcccg gcgtgccgcg ccttgcgatc gaggccggcg
     1201 cgaccgatgg ctggcgcaaa tacgtcggcg cggccgacga tttgcacgcc gctgtcatcg
     1261 gcatcgaccg cttcggcgaa tcggcgcccg ccaatgcgct gtttccgttc ttcggcttca
     1321 ccgtcgacaa cgtcgtcgag gcggtgcgcc gcatcagcgg ccggcaacga ccggcgccat
     1381 gaccctcacg atccgcgcag cgagcgtcga cgacgcgcag ccgatcgccc gcattcgcat
     1441 cgactgctgg cgcagcacgt accgcgggct gattcccgac gcgtatctcg atgccatgga
     1501 gatcgacgcc agcgccgcgt tgtggacacg cgtgctcgca acttcaacgg acaaagcgag
     1561 cgtgttcgtc gccgacgacg atggcgagat cgtcggcttc gccgccggca acatgctcga
     1621 ggaaccccgg cacggactcg atgccgaact gtccgcggct tacgtgcggc gcgaccatca
     1681 gcgcgcgggc atcgggcggc gcctcgtcga cgcggtggcg cgcgcgcaac gagcgcaggg
     1741 tgcccgcggc ctgatcgtct gggtgatcgc cgggaacaag ggcgcccggg cgttctacga
     1801 agggctcggg gcgacgcttc tgatcgagca gccgttcgaa tgggacggca tgccgctcat
     1861 cgaggcgggc tacggatatt ccgacatcga cgcgctgata cgggcgtgcg aaaccggcgg
     1921 agcacacgga tcgaccgtgc attgagacgg agcacacgga tcgaccgtgc attgatctac
     1981 aacgccccgc gtgcggatgc gagcaggcgc accccgggac acgacgaaga ggagttcgca
     2041 tgacgatcaa ggttgccatc aacggttacg ggcgcatcgg gcgcaacatc cttcgcgccc
     2101 actacgagga tggcaagaag cacgatcttg cgatcgtcgc catcaacgat ctcggcagtc
     2161 ccgagacgaa cgcgcatctg acgcgccacg acaccgcaca cggccacttc cccggccgga
     2221 tcgacgtcga gggcgatgcg atggtggtca acggcgatcg catccgcgtc ttcgccgagc
     2281 gcaatccggc gcaactgccg tggggatcgc tcggcgtcga cgtcgtgctc gagtgcaccg
     2341 gattgttcac gacgaaggaa aaggcgtcgg cgcatttgaa ggccggtgcg cgcaaggtga
     2401 tcatctccgc gcccggcggc aaggacgtcg acgcaacgat cgtctacggc gtcaaccacc
     2461 agaagctccg ggcgtcggac acggtgatct cgaacgcgtc gtgcacgacg aactgcctcg
     2521 ctccgctcgt caagccgctg cacgagcgga tcggcatcgt caacggcctg atgacgaccg
     2581 tccatgccta cacgaacgac caggtgctga ccgacgtcta tcacgaggac ctgcgccgcg
     2641 cgcgctcggc gacgatgagc atgatcccga cgaagaccgg cgcagcgtcg gcgctcgggc
     2701 tcgtgctccc ggagatggcc ggcaggctcg acggctactc gatccgcgtg ccgacgatca
     2761 acgtgtcgct cgtcgacctt tcgttcgtcg ccgcgcgcga cacgagcgtc gaggaagtga
     2821 accgcgtgat gaaggaggcg tcggaatcgg gaccactgtc cggcggcatc ctgcgctaca
     2881 acatcgagcc gctcgtgtcg gtcgacttca accacgatcc cgcatcgtcc acgttcgatg
     2941 cgacgctcac gaaggtgtcg ggccggctcg tgaaggcctg cagctggtat gacaatgaat
     3001 ggggattcag caatcgaatg ctggacgtca cc
//
LOCUS       DASSVX010000163         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_389800, whole genome shotgun sequence.
ACCESSION   DASSVX010000163 DASSVX010000000
VERSION     DASSVX010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389800"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFO53_06950"
     CDS             <1..690
                     /locus_tag="VFO53_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8876050.1"
                     /translation="QAEQSKYRTVGVYAGALGYSYNTEQLKKKNIAPPKCWSDLIKPE
                     FKDEIQVANPNSSGTSYTMLATLVQLMGEDKAFDYLKALHKNVNQYTKSGAAPARAAA
                     TGESLVGITFQHDAVVQAVGGAQVTIVSPCEGTGYEIGSMSIIKGAKNMENAKKWYDW
                     ALTPEAQAIGAQAKVSYQVPSNKSVTPPPQAPKFSEIKLINYDFAKYGSSAERTRLLT
                     KWDKEVYALPK"
     gene            692..>2413
                     /locus_tag="VFO53_06955"
     CDS             692..>2413
                     /locus_tag="VFO53_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET8876051.1"
                     /translation="MATPAAALPGTGPTRGSALDPVAALIAVTGIAALAVIPWSLDGG
                     SSAFALVASGAKWPRYAASLALGPLFVAAVAMLVAGFLRSPRIAAMAAGFGLVWGFAQ
                     GLIAAGNGPPFGVGAALTLSAFTVGFARALARLGWFSANATIATIVVVIATLLIVFIF
                     YPVGSALVAAVLDARGKFAPGLVGERLLTADIWGFGCFGGGTRCGVAINSALLASIVG
                     VLSTLLGLVLALVVQRGGKRYAGLLKVMSILPIITPPFVIALALVVLFGRTGLVTSWL
                     DAAFGIGRSRWIYGLPGVALAQLLTFTPVAFMLLFGALGAISPALEEAAQTLRASRGR
                     VFRTVTWPLLRPALANAFLLGFVESLADFGNPIVLAGNFDVLSTKIFFAVAGAQYDAG
                     RAAVLAMVLLGLTLLAFFLQQRWVGRLSYVTVSGKGDGGAPADLPRKLWLACFGIAAV
                     WIVFTLVCYAVILTGGFVRDIGRGDMTPTITHLGAGFAAEWTSRGLLFSGSAWDSLFN
                     TIEVAAIAAPLTALVGLLSAYVITRHRFAGRRAFEFLTLVSFAVPGTVIGVSYIVAFN
                     VAPLELTG"
BASE COUNT          355 a          790 c          802 g          466 t
ORIGIN      
        1 caggcggagc aatcgaagta tcgaaccgtc ggcgtttatg cgggcgcgct cggctacagc
       61 tacaacaccg aacagctcaa gaaaaagaac atcgcgccgc cgaagtgctg gtcggatctg
      121 atcaagcccg aattcaagga cgagatccag gtcgcgaacc cgaattcatc cggcacgtcg
      181 tacacgatgc tcgcgacact cgttcaattg atgggcgagg acaaggcgtt cgattacctg
      241 aaggcgctgc acaagaacgt caaccagtac acgaagtcgg gtgcagcgcc cgcgcgcgca
      301 gccgcgaccg gtgagagcct cgtaggcatc acgttccagc acgatgccgt cgtgcaagca
      361 gtgggcggcg cccaggtgac gatcgtgtcg ccctgcgaag gcaccggcta cgagatcggc
      421 agcatgagca tcatcaaggg cgcgaagaac atggagaacg cgaagaaatg gtacgactgg
      481 gcgctgacgc cggaggcgca ggccatcggc gcgcaggcga aggtctcgta ccaggtgccg
      541 tcgaacaaga gcgtgacgcc gccaccgcag gcgccgaagt tctccgagat caagctcatc
      601 aattacgact tcgcaaaata tggctcatcg gcggagcgca cgcgtcttct gacgaagtgg
      661 gacaaggagg tctacgcgct gccgaagtaa cgtggccacg cccgcggcag cgctgcccgg
      721 gaccggtccc acccgcggca gcgcgctcga ccctgtcgcg gcgctgattg ccgttacggg
      781 gatcgccgcg ctcgcggtga tcccctggtc gctcgacggc ggatcctcag cgttcgcgct
      841 cgtcgcctcg ggcgccaaat ggccgcgcta cgccgcgagc ttggcgctcg gaccgttgtt
      901 cgtcgctgcc gtcgccatgc tcgtggcggg ctttctgcgc agcccgcgca tcgccgcaat
      961 ggccgccggc ttcggcctcg tctggggctt cgcgcaaggc ttgatcgccg ccggcaacgg
     1021 cccgccgttc ggcgtcggtg cagcgctgac gctctccgca ttcaccgtcg ggttcgcgcg
     1081 cgcgctcgcg cgactcggct ggttcagcgc gaatgcgacg atcgcgacga tcgtcgtcgt
     1141 catcgcgacg cttctgatcg tcttcatctt ctacccggtc ggcagtgcac tcgttgccgc
     1201 cgtgctcgat gcgcgcggca agttcgcgcc gggcctcgtc ggcgagcggc tgttgacggc
     1261 cgacatctgg ggctttggtt gcttcggtgg cggaacgcgt tgcggcgtcg cgatcaactc
     1321 ggcgctgctc gcgtcgatcg tcggcgtttt gtcgacgctg ctcgggctcg tgctcgcgct
     1381 cgtcgtccag cgcggcggca agcgttacgc gggccttttg aaagtgatgt cgattctccc
     1441 gatcatcacg ccgcctttcg tcatcgcgtt ggcgctcgtc gtgttgttcg gccgcacggg
     1501 attggtgacg agctggctcg acgccgcgtt cggcatcggc cgctcgcgct ggatctacgg
     1561 cctcccgggt gtcgcactcg cgcagttgct gaccttcacg ccggtcgcat tcatgctgct
     1621 gttcggcgcg ctcggtgcga tcagtcctgc gctcgaggag gcggcacaga cattgcgcgc
     1681 gtcacgcggc cgcgtgtttc gcaccgtcac ttggccgctc ctgcgcccgg cactcgcgaa
     1741 tgcgtttctg ctcggatttg tcgaaagcct cgccgacttc ggcaatccga tcgtgctcgc
     1801 gggcaatttc gacgtgctgt ccacgaagat tttctttgca gtcgccggcg cgcaatacga
     1861 cgcgggtcgt gcggcggtgc tcgcgatggt gctgctcgga ttgacgctgc tcgcattctt
     1921 cctgcagcag cgctgggtcg ggcggctgtc gtacgtcacc gtcagtggga agggcgacgg
     1981 cggcgcgccg gccgatctgc cgcgcaagct gtggctcgca tgtttcggca tcgccgccgt
     2041 atggatcgtg ttcacgctcg tctgttatgc ggtgatcctt accggcgggt tcgtgcgcga
     2101 catcggccgc ggtgacatga cgccgacgat cacgcatctt ggcgcgggct tcgcggccga
     2161 atggacgtcg cgcggattgc tgttttcggg ctcggcgtgg gacagcctgt tcaatacgat
     2221 cgaggtcgcg gcgatcgccg ccccgttgac ggcgctcgtc gggctcctgt cagcgtacgt
     2281 gatcacgcgc catcgcttcg ccggccgccg cgcgttcgag tttctgacgc tggtcagctt
     2341 cgccgtgccg ggcaccgtca tcggcgtctc gtacatcgtc gcgttcaacg tcgcaccgct
     2401 cgagctgacc ggt
//
LOCUS       DASSVX010000164         8039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1056436, whole genome shotgun sequence.
ACCESSION   DASSVX010000164 DASSVX010000000
VERSION     DASSVX010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056436"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..470
                     /locus_tag="VFO53_06960"
     CDS             51..470
                     /locus_tag="VFO53_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HET8876052.1"
                     /translation="MATAKKAAKKTAAKKPAARKSAAKKPAAKKSAAKKPAAKKSAAK
                     KPAAKKSAPAAKRKPNAAFMKAMQPSSQLAAVVGNAPMPRTEVTKKIWDYIKKNKLQD
                     GVNKRLINADDKLRLVFGGKNKVSMFEMTKLVSNHLK"
     gene            complement(610..840)
                     /locus_tag="VFO53_06965"
     CDS             complement(610..840)
                     /locus_tag="VFO53_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876053.1"
                     /translation="MRYKIEVQDDTGLWADVRGDDGALLVFDDEQAARAALAERFPIL
                     VQMEKYAGGKRTRVIRILEDEDDWPARRRDPE"
     gene            complement(843..1718)
                     /gene="htpX"
                     /locus_tag="VFO53_06970"
     CDS             complement(843..1718)
                     /gene="htpX"
                     /locus_tag="VFO53_06970"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HET8876054.1"
                     /translation="MKRIILFVATNLAVVLVLSIVLRIFGLDRALAAQGFQYGQLLVY
                     SVVVGFTGAIISLLMSKSIAKWTTGAHVIETPANEAEAWLVETVRKLATSVGIAMPEV
                     GIYEGAANAFATGAFKNSAFVAVSTGLLQAMSHEEVEAVLGHEISHVANGDMVTLTLI
                     QGVVNTFVVFLSRIVGSIVDRTVFRTERGSGPGYFITVIVCQIVFGILASVIVAWFSR
                     WREFRADAGSARLLGAQPMIHALQRLGGLDAGGLPQAMNAFGIAGSGGFMALFASHPP
                     IEERIAALQSAPAGR"
     gene            complement(1870..3744)
                     /locus_tag="VFO53_06975"
     CDS             complement(1870..3744)
                     /locus_tag="VFO53_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004535600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HET8876055.1"
                     /translation="MNKTVHAAPRGDSVEAAPVSSTLGDAAPVRDDARGVGANFLRTI
                     VAEDNASGVYGGRVVTRFPPEPNGYLHFGHAKSIVLNFGLAAENGGTCHLRYDDTNPL
                     KEDVEYEDSIAASVRWLGYDWGTHRYHASDYYDTLYAFGEWFVSQGLAYVDSQSAEAL
                     RRTRGTLTEPGVPGPWRERSIAENLDLLRRMRAGEFADGTHVLRLRIDMASPNLNMRD
                     PVIYRIRHARHHRTGDKWCIYPLYDYTHCISDALERITHSLCTLEFQDHRPLYDWIIE
                     RLAEGGKLARPLPHQYEFARLNLTYVVLSKRKLLRLVEEGRVDGWDDPRMPTLVGARR
                     RGYTPSGLRAFNERIGVSKSDSWIDMGVLEDAMRDDLNASAPRRMAVLDPVRLVIDNY
                     PAGESEECEAPNHPQHPELGRRTLPFSGELWIERDDFTEVPPKGYFRLSPGAEVRLRY
                     AYIVRCTGVEKDDAGRVTVVHCTYDAATRSGTAGADARKVKGNIHWLSIAHAVCAEVR
                     LYDRLFGVPFPGAREARAGGGEAVSAALPAHPIGVAGDDDDIGDERDFLDDLNPESKR
                     IIAAYVEPMLAVAAAEERFQFERHGYFVADLAEHASGRPVFNRTVTLRDSWAKPGARR
                     "
     gene            4184..5578
                     /gene="xseA"
                     /locus_tag="VFO53_06980"
     CDS             4184..5578
                     /gene="xseA"
                     /locus_tag="VFO53_06980"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET8876056.1"
                     /translation="MNDDAMRETDPGEVTGTALPRPGAPVMPVSLFVSSARLLLERHL
                     GLMWIGGEISGCTRAASGHIYFTLKDAKAQIRCVFFRHKAQGLSFTLREGLGVEVRAV
                     ASIYEPRGEFQLNVETVRLAGLGALYERFVARKRALEAAGLFDSARKRALPAFPRRIG
                     IVTSRAAAALRDVLTTLARRSPGVAVILYPASVQGAGAAAEIAAAIRLANAHAKVDAL
                     IVCRGGGSLEDLWAFNEEVVARAIFESRLPIVSGVGHETDFTICDFVADVRAATPTAA
                     ASLAVPERAALHAAIGALSRRLARAHAHRAGNCAQRLDAASRRLVHPAARLAQQGERA
                     RALGRRLERAFGHDRAAAAQRLHALRARLLRELRTPLAQAARVDRVAHALARSAQGRL
                     AGDARKAERLGEALALLNPEAVLERGYAIVTAHDGRVIADACEMRVGDNIALTLARGR
                     ALASVTATDVERDR"
     gene            complement(5547..6290)
                     /locus_tag="VFO53_06985"
     CDS             complement(5547..6290)
                     /locus_tag="VFO53_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8876057.1"
                     /translation="MTDTHQLVDALKKLLKAQGLTYSVVATRLGLSHASVKRLFAAET
                     FTLARLEQLCRLLEVDFFDLARLARGRGESTREMTERQEAALARDAKLLGVFYLLLSD
                     WTADEIVAGYQIGRPELTRLLVQLDRLQLIELMPRDRVRLRVPKLLQLRRGGPIHRAH
                     GKRVVDEFIAAEFDRVGGQFGFEYRELSKASYALMQRRLERLAAEFLELAELDAAMPP
                     ARRETIGLVVAMRPWALSLVTGLAPRRSP"
     gene            6412..6846
                     /locus_tag="VFO53_06990"
     CDS             6412..6846
                     /locus_tag="VFO53_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876058.1"
                     /translation="MNVKRTLVALAAAGCIASFAATPAAATDSTAASAEASQAAIAGS
                     GMIVDGSAQLILAGGALTIAGISAAGDASVIVLRDVATGSEASMRVGADVAQAGSLAV
                     GQTVSVVAEATGMSLFAGGRLVAFVPSEIGRALVYAARSTQG"
     gene            6857..7720
                     /locus_tag="VFO53_06995"
     CDS             6857..7720
                     /locus_tag="VFO53_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2145 domain-containing protein"
                     /protein_id="HET8876059.1"
                     /translation="MLRAILAFTFAFVSFTVHAGQACTEKRLSPAEVRAALTLALDVT
                     RALDGAGAHVAIVARAGRDLSRYGLRYSHVAFAWRDHPRGAWTMVEELNRCGSADSAL
                     FDDGPGDFFLDDMFAYEAAIVVPSPDGQRRIGALLAAHAGGRLHEPRYNLVAYPWSTR
                     YQNSNQWVLETIAAAWSDRPVPDRAQAQAWLRAEGFRPATIHLTPIERFGSELTRANV
                     AFDDHPLDRRMAGRIDVVSAESVLDFVRRIDPGASALVVSVDTPIAQAPRRAYDTTPR
                     EPGRYEQRRTR"
     gene            7717..>8039
                     /locus_tag="VFO53_07000"
     CDS             7717..>8039
                     /locus_tag="VFO53_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876060.1"
                     /translation="MKVTLDLDALLGAGRIDQREYDKLRAFSKEGTSALAFNILVAFG
                     VVAVAGATLALLPTALTAVALGAAICAAGLALVLKRDVQWHVLAVVCVVAGALLFGGG
                     IVKLG"
BASE COUNT         1322 a         2762 c         2701 g         1254 t
ORIGIN      
        1 ccgaggacgc tgcagcgtac cgcccgcaaa aactgacagg aaggagaaac atggctactg
       61 ccaagaaagc tgcgaaaaag accgcggcca agaagccggc cgccaggaag agtgctgcaa
      121 agaagccggc cgcgaagaag agcgcagcga aaaaacccgc cgcgaaaaaa agcgcagcga
      181 agaagcccgc ggcgaagaag tcggcgccgg cggccaagcg caagccgaac gcggccttca
      241 tgaaggcgat gcagccgtcc tcgcaactcg ccgccgttgt cggcaatgcg ccgatgccgc
      301 gcaccgaagt tacgaagaag atctgggact acatcaagaa gaacaagctg caggacggcg
      361 tgaacaagcg gctcatcaac gccgacgaca agctgcgcct ggtgttcggt ggcaagaaca
      421 aggtgtcgat gttcgagatg acgaagctcg tttcgaatca cctcaagtaa cgcgagagca
      481 ttcacgcgcg cggccttcgc gcgcactgga cggccggcgc actcgcttac gggtggccgg
      541 ccgtttcgct tgcgccgcgc gggccccgct cgcgcgtctg tctccacgct tgcggctttg
      601 ccgctccgct cattcggggt cgcgccggcg tgccggccag tcgtcctcgt cctcgaggat
      661 gcggatcacg cgcgtgcgct tgccaccggc gtacttctcc atctgcacga ggatgggaaa
      721 gcgctcggcg agcgccgcgc gcgccgcctg ctcgtcgtcg aacaccagca gcgcgccgtc
      781 gtcgccgcga acgtcggccc agaggccggt gtcgtcctgc acctcgatct tgtagcgcat
      841 cgtcagcgtc ctgcaggcgc cgattgcagc gccgcgatgc gctcctcgat cggcggatgc
      901 gacgcgaaaa gcgccatgaa accgccgctg ccggcaatgc cgaacgcgtt catcgcctgc
      961 ggaaggcctc ccgcatcgag gccgccgagg cgttgcagcg catggatcat cggctgcgcg
     1021 ccgagcaggc gcgcactgcc ggcgtcggcg cggaactcgc gccagcgcga gaaccacgcg
     1081 acgatcacgc tcgcgaggat gccgaacacg atctggcaca cgatcacggt gatgaagtag
     1141 cccgggccgc tgccgcgctc ggtacggaac accgtgcggt cgacgatcga gccgacgatg
     1201 cgcgacagga acacgacgaa cgtgttgacc acgccctgaa tcagcgtcag cgtgaccatg
     1261 tccccgttcg ccacgtgcga gatctcgtgg ccgagcaccg cctcgacctc ctcgtggctc
     1321 atcgcctgca gcagtccggt ggacacggcg acgaacgccg aattcttgaa cgcacccgtt
     1381 gcgaacgcgt tcgcggcgcc ctcgtagatg ccgacctcgg gcatggcgat gccgaccgac
     1441 gtggcgagct tgcgcaccgt ttcgacgagc cacgcctcgg cctcgttcgc cggcgtttcg
     1501 atcacgtgcg cgccggtcgt ccatttagcg atcgacttcg acatcagcag cgaaatgatg
     1561 gcgccggtga agccgacgac gaccgaatag acgaggagct gcccgtactg gaagccctgc
     1621 gcggcaagcg cgcgatcgag cccgaagatg cgcagcacga tcgacagcac gagaacgacg
     1681 gcgagattcg tggcgacgaa caggatgatc cgcttcatgg ggtcctcgac ggttgccgtc
     1741 ggcgtcgcgc gcggccgatg acgacccgcg tgcggccgaa ggagtgaact tcgcgccatc
     1801 gcatgacgaa tgcgcggtcc gaatattagc gcggcaaccg cgcttcacaa ggcccggtgc
     1861 cgtgcgcgcc tagcggcggg cgccgggctt cgcccacgaa tcgcgcagcg tcaccgtgcg
     1921 gttgaacacc gggcggccgc tcgcgtgctc ggcgagatcc gcaacgaaat agccgtgccg
     1981 ctcgaactgg aagcgttcct cggcggccgc aacggcgagc atcggctcga catacgcggc
     2041 gatgatgcgt ttcgactcgg ggttgagatc gtcgagaaag tcacgctcgt cgccgatgtc
     2101 gtcatcgtcg cctgcaacgc cgatcgggtg cgcgggcaac gcggcgctca cggcctcgcc
     2161 gccgcccgct cgcgcctcgc gcgcgccggg aaacggaacg ccgaacagcc gatcgtaaag
     2221 acgcacctcg gcgcacaccg cgtgggcgat cgacagccag tggatgttcc ccttgacctt
     2281 gcgcgcgtcg gcaccggcgg tgccgcttcg cgtcgcggca tcgtaggtgc agtgcacgac
     2341 cgtcacgcgc ccggcgtcgt ccttctcgac gccggtgcag cggacgatgt acgcgtagcg
     2401 caagcgcacc tcggcacccg gcgacaggcg gaagtagccc ttcggcggca cctcggtgaa
     2461 atcgtcgcgc tcgatccaca gctcgccgct gaacggcagc gtgcgccgcc ccagctcggg
     2521 atgctgcgga tggttcggcg cctcgcattc ctcgctttcg ccagcgggat aattatcgat
     2581 gacgagccgg acgggatcga ggacggccat ccggcgcggc gcgctcgcgt tgaggtcgtc
     2641 gcgcattgcg tcctcgagca cgcccatgtc gatccacgaa tcggacttcg acacgccgat
     2701 gcgctcgttg aacgcgcgca atcccgacgg cgtatagccg cgccgccggg cgccgacgag
     2761 cgtcggcatc cgcggatcgt cccagccgtc gacgcgaccc tcttcgacga gccgcagcag
     2821 cttgcgcttc gacagcacga catacgtgag gttgagacgc gcgaattcgt attggtgcgg
     2881 cagcggccgc gcgagcttgc cgccctcggc gaggcgctcg atgatccagt cgtacagcgg
     2941 ccgatgatcc tggaactcga gcgtgcacaa cgaatgggtg atccgctcga gtgcgtcgga
     3001 gatgcaatgc gtgtagtcgt agagcgggta gatgcaccat ttgtcgccgg tgcggtggtg
     3061 gcgcgcatgc cggatgcggt agatcaccgg atcgcgcatg ttgaggttcg ggctcgccat
     3121 gtcgatgcgc aggcgcagca cgtgcgtgcc gtcggcgaat tcgccggcgc gcatgcggcg
     3181 cagcagatcg aggttttcgg cgatgctgcg ctcgcgccac gggccgggca cgccgggttc
     3241 ggtcagcgta ccgcgcgtgc ggcgcagcgc ttccgccgac tggctgtcga cgtacgcaag
     3301 gccctggctc acgaaccatt cgccgaacgc gtacagcgtg tcgtagtaat cggacgcgtg
     3361 ataccggtgc gtgccccagt cgtagccgag ccagcgcacc gaggccgcga tcgaatcctc
     3421 gtactcgacg tcttccttca gcggattggt gtcgtcgtaa cgaaggtggc acgtgccgcc
     3481 gttctcggcg gcgaggccga aattcaagac gatcgacttc gcatgaccga aatgcaggta
     3541 gccgttcggc tcgggcggaa agcgcgtgac gacacgcccg ccgtagacgc cgctcgcgtt
     3601 gtcttcggcg acgatcgtgc gcaggaagtt ggcgcccaca ccgcgggcgt cgtctcgtac
     3661 cggagcggca tcgccgagcg tcgacgacac cggagcggct tcgacgctgt cgcctcgcgg
     3721 tgcagcatgg acagttttgt tcatgaacaa gtacctgatc cgggaattct aaccggcggc
     3781 cgatgtcacg tcaccggccc ggttcacagg gcacccgcgt cgcaccccgg aaacccgcgc
     3841 cgggccacgc tttgcgcgga ggtgtcccgt cgcgcgcggc aacgttgtcc acaaatcctg
     3901 tggatagcaa tgtgcatatc ttgtaaattc gcttgctaag tgctgtgccg gtcagggaaa
     3961 tcggccccat gacgaaaatc gtgtcgtgaa aaagagacgc caattcaagg acctgcgagg
     4021 cgatctccgc gggtgtttgc gcgtcgatcg acgcgcagca ttgcgccggc caagcccctg
     4081 atccggccga tttgcacgac attgcagcaa ctatgtccag gacatcccca agtttttgtc
     4141 caccacagtc ggcgcgacgc tgcgaacggc gtcacgatga gcgatgaatg atgatgcaat
     4201 gcgggagacc gaccccggcg aggtgaccgg gaccgcgttg ccgcgcccgg gcgcgccggt
     4261 gatgccggtg tcgctgttcg tctcctccgc caggctgctt ctcgagcgac atctgggact
     4321 gatgtggatc ggcggcgaga tatcgggctg tacgcgcgcc gcgtcgggcc acatctattt
     4381 cacgctgaag gacgcgaagg cgcagattcg ctgcgttttc ttccgccaca aggcgcaagg
     4441 cctctccttc acgctgcgcg aggggctcgg cgtcgaagtg cgtgccgtcg cgtcgatcta
     4501 cgagccgcgt ggggaattcc agctcaacgt ggaaacggtg cgtcttgcgg gccttggcgc
     4561 gttgtacgag cgcttcgtcg cgcgaaagcg tgcgctcgaa gccgccggcc ttttcgacag
     4621 cgcgcgcaag cgcgcgttgc ccgcgttccc gcggcgcatc ggcatcgtga cgtcgcgcgc
     4681 ggccgccgca ttgcgcgacg tgctgacgac gctcgcacgg cgctctcccg gcgtggcggt
     4741 gatcctgtat ccggcgtcgg tgcaaggcgc gggcgccgcg gcggaaatcg ccgccgcgat
     4801 ccggcttgcc aacgcgcacg ccaaggtcga cgcgctgatc gtgtgccgcg gcggcggctc
     4861 gctcgaggat ctgtgggcgt tcaacgagga agtggttgcc cgcgccatat tcgaatcgcg
     4921 gttgccgatc gtctccggcg tcggacacga gaccgatttc acgatctgcg acttcgttgc
     4981 cgacgtgcgc gcggcgaccc ctaccgccgc cgcctcgctg gccgttcccg agcgcgccgc
     5041 gctgcatgcc gccatcggtg cgctgtcccg gcgcctcgcc cgcgcccatg cgcatcgcgc
     5101 cggaaactgc gcacagcgcc tcgatgccgc gtcgcgccgt ctcgtgcacc cggccgctcg
     5161 cctcgcccag caaggcgagc gtgcacgcgc cctcggccgg cggctcgagc gcgcgttcgg
     5221 gcacgaccgc gccgccgccg cacaacgcct gcacgcattg cgtgcccgcc tgctgcgcga
     5281 gctccgcacg ccgcttgcgc aggccgcacg cgtcgaccgg gttgcccatg cgctcgcgcg
     5341 aagcgcgcag gggcgtctcg cgggtgacgc gcgcaaagcg gagcgcctcg gcgaggcact
     5401 cgcgttgctc aatcccgaag cggtgctcga gcgcggctac gcgatcgtca ccgcgcacga
     5461 tggccgcgta atcgccgacg catgcgagat gcgtgtcggc gacaacatcg cgttgacgct
     5521 tgcgcgcgga cgcgcgctcg cgagcgtcac ggcgaccgac gtggagcgag accggtgacg
     5581 agcgacagcg cccacgggcg catcgcgacg acgagcccga tcgtttcgcg acgcgccggc
     5641 ggcatggcag catcgagctc ggcgagctcg aggaactcgg cggcgagccg ctccagccgt
     5701 cgctgcatca gcgcataaga ggccttcgaa agctcccggt actcgaaacc gaactgaccg
     5761 ccgacgcggt cgaactcggc ggcgatgaac tcgtcgacga cgcgcttgcc gtgcgcgcgg
     5821 tggatcggac cgccgcgccg aagctgcaac agtttcggca cgcgcaagcg cacgcggtcg
     5881 cgcggcatga gctcgatcag ctgcaaacgg tcgagctgca cgagcagccg cgtgagctcg
     5941 ggccggccga tctggtaacc ggcaacgatc tcgtcggccg tccagtcgga aagcagcagg
     6001 tagaagacgc caaggagctt cgcgtcgcgc gccagcgccg cctcctgccg ctcggtcatc
     6061 tcgcgcgtcg attcgccacg cccgcgcgcg agccgcgcaa ggtcgaagaa gtcgacctcg
     6121 agcagccggc acagctgctc gagacgcgca agcgtgaacg tttcggccgc gaacagccgt
     6181 ttcacgctcg cgtgcgaaag cccgagtcgc gtcgcgacga ccgaataagt gaggccctgc
     6241 gccttcagca gcttcttcaa tgcgtcgacg agctggtggg tatcggtcac cgggtgattc
     6301 cgcgggtatc gtttttcgat actctacatg aagcgtattg accggtgcta ccaacggcgg
     6361 acactccggc tcgcgggcag accccgcgtt cacccacaac cggagaaatc catgaacgtg
     6421 aagcgcaccc ttgttgccct cgccgccgcc ggctgcatcg catcgttcgc ggcgacgccc
     6481 gctgcggcca ccgatagcac cgccgcctcg gccgaagcgt cgcaggccgc aatcgccgga
     6541 tcgggaatga tcgtggacgg cagcgcgcaa ttgatcctcg ccggcggcgc gttgacgatc
     6601 gccggcatca gcgcagccgg cgacgccagc gtgatcgtgc tgcgcgacgt tgcaaccggc
     6661 tcggaagcgt ccatgcgcgt cggcgccgat gtcgcgcaag cgggctcgct tgcggtcggc
     6721 cagacggtca gcgtcgtcgc cgaggcgacc ggcatgtcgc tcttcgccgg cggacggctc
     6781 gtcgccttcg ttcccagcga gatcggccgc gcactcgtgt acgccgcgcg ttcgacgcag
     6841 gggtaagggg accgtcatgc tgcgcgccat cctcgcattt acgtttgcgt tcgtctcctt
     6901 caccgtgcac gccgggcagg cgtgcaccga gaagcggctg tcgcccgcgg aggtgcgcgc
     6961 ggcgctcacg ctcgcgctcg acgtcacgcg ggccctcgat ggggcgggcg cacacgtcgc
     7021 aatcgttgcc cgcgccgggc gcgacctgtc gcgctacggc ctgcgctact cgcacgtcgc
     7081 cttcgcgtgg cgcgatcatc cgcgcggcgc atggacgatg gtcgaggagc tcaaccggtg
     7141 cggcagcgcc gattcggcgc tgttcgacga tggtcccggc gatttcttcc tcgacgacat
     7201 gttcgcctac gaggcggcga tcgtcgttcc gtcgcccgac gggcagcgca ggatcggcgc
     7261 gctccttgca gcccacgccg gcggccggct gcacgagccg cgctacaacc tcgttgccta
     7321 tccgtggagc acgcgctacc agaactcgaa ccagtgggtg ctcgaaacga tcgccgcggc
     7381 gtggtccgat cgtccggtgc cggaccgcgc gcaggcgcag gcctggctgc gggccgaagg
     7441 cttccgtccg gccacgattc atctcacgcc aatcgagcgt ttcggcagcg agctcacgcg
     7501 ggcgaacgtc gccttcgacg accatccgct cgatcggcgg atggcgggac ggatcgacgt
     7561 cgtcagcgcc gagtcggtgc tcgatttcgt ccgccggatc gacccgggcg cgtcggcgct
     7621 cgttgtgtcg gtcgacacgc ccatcgcgca agcgccccgc cgcgcgtacg atacgacgcc
     7681 gcgcgaaccg ggccgatacg aacaacggag gacccgatga aagtcacgct cgacctcgac
     7741 gcgctgctcg gtgccggccg catcgaccag cgcgaatacg acaagctgcg cgcgttctcg
     7801 aaggaaggca cgtcggcgct cgcgttcaac atcctcgttg cattcggcgt cgtcgcggtt
     7861 gccggcgcaa cgctcgccct gctacccacc gcgctcaccg ccgtcgcgct cggtgcggcc
     7921 atctgcgccg cgggactcgc gctcgtgctg aagcgtgacg tccagtggca cgtccttgcc
     7981 gtcgtatgcg tcgtcgccgg ggcgctgttg ttcggcggcg gcatcgtcaa gctcggcga
//
LOCUS       DASSVX010000165         9726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_189765, whole genome shotgun sequence.
ACCESSION   DASSVX010000165 DASSVX010000000
VERSION     DASSVX010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189765"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..1629
                     /locus_tag="VFO53_07005"
     CDS             337..1629
                     /locus_tag="VFO53_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015879402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HET8876061.1"
                     /translation="MPLCVWAQGGAPEPPAIGTIPIDFILFALTLVGVALFHHHTLKV
                     ALIGLGVITFFKIAFSPFPEGAGVGGFAAHLGHEWVLLTNLLGLLLGFALLSKHFEAS
                     QVPAWLPKLLPDDWKGAFVLLVMIFVLSSFLDNIAAALIGGTIAGVVFRRKVHIGYLA
                     AIVAASNAGGSGSVVGDTTTTMMWIDGVSPLAVLEAYIAASLALVLFGIPAAMQQQRY
                     SPITRDSGANVRIDLVRVFIVATILITAIVVNVVVNVRFNAWSDRFPFIGAAVWVALF
                     ACVPLRKPHWEELPGALKGSIFLLSLVLAASMMPVHKLPDASWHVALGLGFVSAVFDN
                     IPLTALALKQGGYDWGYVAFAVGFGGSMIWFGSSAGVALTNLFPEAKSVFAWLRGGWH
                     VAIAYVLGFALMLAVHGWAPDAPHRAPPNAPVAAAPGA"
     gene            1736..2209
                     /locus_tag="VFO53_07010"
     CDS             1736..2209
                     /locus_tag="VFO53_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HET8876062.1"
                     /translation="MKHTALALAMLLVSATAASAEGPRQPLAANEGARGSTVVQNDFW
                     VSTQDRERRRDERRRGIGAILYSNPDLRGERYIIEGEYMRNLGNTGFNDRAQSLQVER
                     GYWIFCSDAEFQGTCRTFGPGEYRRLPRGLDNRISSGRRISENYPYQGRPNWERR"
     gene            2214..2687
                     /locus_tag="VFO53_07015"
     CDS             2214..2687
                     /locus_tag="VFO53_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET8876063.1"
                     /translation="MPIVDDIAGLRRILARSRTLAVVGLSAQWYRPSYFAAKYMQDHG
                     YRIIPVNPRYDEVLGQKCFPDLRSIAQPVDLVDCFRKPQDIPPIAEDAIAIGAKVLWM
                     QLGIENRDAAQRAGDAGLEVVMNRCVKIEHARILGGLNWAGVNTGVISSRRPSAS"
     gene            2804..4090
                     /locus_tag="VFO53_07020"
     CDS             2804..4090
                     /locus_tag="VFO53_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HET8876064.1"
                     /translation="MPDHAYGFDTLCLHAGQIPDAQTGARAMPIYQTTSFVFDDADHA
                     ASLFNLQTFGNVYSRISNPTVAALEERVAALEGGRAALAAATGMAAQMLTFLTLARAG
                     DHIVAARTLYGGSYSQLAVTFAQLGIDSTFVDAGDPENFRHALKPSTKCLYAETIGNP
                     QLNVPDIAALAEIAHGAGVPLVIDNTLASPYLCRPFEHGADIVVHSLTKYLGGHGTTM
                     GGIVVESGKFPWDNGNFPQMTEPSRGYHGVIFHETFGDFGFTMKARMETMRTLGPTLS
                     PLSAFLLLQGVETLHLRMPRHCESALAVAQHLERHPAVEWVNYPLLPGNPGHALAQRY
                     LPRGAGGILTFGVKGGAAAGVRCIEAVQFLSHLANVGDAKTLIIHPASTTHRQLDEGE
                     QRAAGVSPEMIRLSVGLESLDDILWDLDQALEQSQR"
     gene            4148..4801
                     /locus_tag="VFO53_07025"
     CDS             4148..4801
                     /locus_tag="VFO53_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HET8876065.1"
                     /translation="MNRYASIPWPYAALFLCAALIAQEFLLRSFGAQPAMSESAYRVF
                     VMTLGVASLALIVLPPRRTAYLLGFIVCAALMAWALWLQYGEGLEPCPLCMFQRVCVS
                     AVGIVFLIAAIQNPGRRSAAFYALLTLIIAGAGAAFAARQIWLQALPKDQVPACGMGL
                     SYMMDTLPFTDVVRKVLEGSGECAEKGWVLLGLSIAGWTFVFFVSMIAAAIALIRRD"
     gene            complement(5003..5935)
                     /locus_tag="VFO53_07030"
     CDS             complement(5003..5935)
                     /locus_tag="VFO53_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876066.1"
                     /translation="MKIYKDRASRCLGLAFLLVGLVVAAPVIAQTRGTIAAFRVQYDI
                     MMANYEQLFATSGNVDGQLRAQRGRAAMQAITDEQLAKVFAKTRIPDFSTVASVAKLL
                     VERKPTQQQKSLPFPGEPAIVNGCVGTDYTSETRYAETIANEVTQSILAAAAWACNED
                     ILGENGSLACVPLAIAADITQGLIDVANFCAGEVTANQVTANLERLAHIHDDLTADVA
                     AITTNINQSTAAINTNINQSTASIISNANTNTTAIINNDNANKDTIVSALSALGCEII
                     RLLNTPEGQRASSILACSAQPGFPYKWNKPGAAR"
     gene            complement(6475..8217)
                     /locus_tag="VFO53_07035"
     CDS             complement(6475..8217)
                     /locus_tag="VFO53_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET8876067.1"
                     /translation="MQHIDLLGSVGIAISVAAILAFIGNRLRQPLLLAYLLAGVLIGP
                     QIGLGLVSDEASIETVSEIGLILLLFIIGLELDLKKLLSAGKPVLVAGVLQFPLCLAL
                     GLAFFLPFGFRMQGGDFSLLYVAVCLALSSTMIVVKLLYDKFELDTLPGRITLGVLIF
                     QDVWAIVVLGIQPNLRNPELGKVLLSLGSGVLLVGATLLISKTVLPRLFRSVAKLPEL
                     VLIGSLAWCFIVCAAASGVGLSREMGALIAGISISTFPYNLDVIAKVVSIRDFFVTLF
                     FVALGMKIPMPTLEIIALALVASLFLVASRFVVVFPILRGLRQGHRTSLLPAINLAQM
                     SEFSMVIAAIGLSYGHIGTKTASLLIFVFAFTSVASTYLIGYSHPLQRTLTRWLRNAG
                     VKDLDDAEPAPDDAHASTTKDVVLLGFFTEASALVHEYEMGETAHAKAMLERLLVIDF
                     NPEVHAELTRRGIACRYGDVANMDTLHHAGVHDARLVVSTIPDAILKGTDNLRLLKLA
                     RRLWPQAKVIVTANRAASALMLYDAGADFVFVSRLHSAAQMAAILEQALDEGFEQMRD
                     EHIGQIRRRREVLA"
     gene            complement(8285..9052)
                     /locus_tag="VFO53_07040"
     CDS             complement(8285..9052)
                     /locus_tag="VFO53_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8876068.1"
                     /translation="MQLRGSTFLVTGGASGLGAATVRMAVDGGGHAVIADLDETRGKA
                     LAGDLGDATRFVRTDVTDEASAEAAIAAALKAFGAIHALVNCAGIVHGEKVVGKEGAH
                     SLATFARAVNINLVGTFNLIRLTAQAMAKNAPNDTGERGVIINTASVAAFDGQIGQAA
                     YSASKAGVVGMTLPIARELARSGIRVMTIAPGLFETPMMASLTPEVQEALGKMVPFPP
                     RLGKPAEFASLVREIVANPMLNGEVIRLDGAIRMAAK"
     gene            complement(9070..9726)
                     /locus_tag="VFO53_07045"
                     /pseudo
     CDS             complement(9070..9726)
                     /locus_tag="VFO53_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394518.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
BASE COUNT         1577 a         3225 c         3136 g         1788 t
ORIGIN      
        1 aacggcgccg gccggaggac ttcgtccggt ccggcgccct cccatccccg ggtctcgccg
       61 ccctgcgtgg cagcaaaaag caaggatcgg gcccggtttg tgacgccgcc ggaccgtccg
      121 gctcccgacg accggccgcg aggcggttac aatccgcgtc accaaaaaac gccgggcggc
      181 agaaccggcc cgcggctacc tcgtgcgtcc gagcagcacc acgacggcag gccactgtcc
      241 acgccaccgg tccgaccgct ctttcacgag gtgccgatgt cgtcatcgcc gaagggacgc
      301 cgtgcggcag cgctcgcgct gccgatcgcg ctgctgatgc cgctttgcgt atgggcgcag
      361 ggcggagcgc ccgagccgcc ggcgatcgga acgattccga tcgacttcat ccttttcgcg
      421 ctgacgctcg tcggcgtcgc gctgttccac caccacacgc tgaaggtcgc gctgatcggc
      481 ctcggcgtaa tcacgttctt caagatcgcg ttctcgccgt ttcccgaagg ggccggcgtc
      541 ggcggattcg ccgcgcatct tggccatgaa tgggtgctgc tcacgaacct gctcggattg
      601 ctgctcggct tcgcgctgct gtcgaagcac ttcgaggcga gccaggtgcc ggcgtggctg
      661 ccgaagctcc ttcccgacga ctggaaaggt gcgttcgtcc tcctcgtgat gatcttcgtg
      721 ctgtcgtcgt tcctcgacaa catcgcggcg gcgctgatcg gcggcaccat tgccggtgtc
      781 gtgttccgcc gcaaggtgca catcggctat ctcgcagcga tcgtcgccgc gtcgaacgca
      841 ggcggctcgg gaagcgtcgt cggcgacacg acgacgacga tgatgtggat cgacggcgtg
      901 tcgccgctcg cggtactcga ggcgtacatc gccgcctcgc tcgcgctcgt gctgttcggc
      961 attccggcgg cgatgcagca gcagcgctat tcgccgatca cccgcgactc gggggccaac
     1021 gtccgcatcg atctcgtgcg cgtcttcatc gtggccacga tcctgatcac ggcgatcgtc
     1081 gtcaacgtcg tcgtcaacgt gaggttcaac gcatggtccg accgctttcc cttcatcggt
     1141 gcggcggtat gggtggcgct ctttgcgtgc gtgccgttgc gcaagccgca ctgggaggag
     1201 ctgcccggtg cgttaaaggg ctcgatcttc ctgctgtcgc tcgtgctcgc agcgtcgatg
     1261 atgccggtgc acaagctgcc cgacgcatcg tggcatgtgg cgctcggcct cggcttcgtt
     1321 tccgcggtgt tcgacaacat cccgctgacg gccctcgcgt tgaagcaggg cggctacgac
     1381 tggggctacg tcgcgttcgc cgtcgggttc ggcggctcga tgatctggtt cggatcgtcc
     1441 gccggcgtcg cgttgacgaa cctgtttccg gaagcgaagt cggtgttcgc atggctgcgc
     1501 ggcggctggc acgtggcgat cgcctacgtg ctgggattcg cattgatgct cgcggtccac
     1561 ggctgggcgc ccgacgcgcc gcatcgcgcg ccgccgaacg cgcccgtcgc cgcagctccg
     1621 ggcgcgtagg agcacgccga cgcggcgatc ggacgccaac cggcttcttg atgcgccgcg
     1681 cgacgattag gatcgtggag gcgacgcata ggcgtcgaaa tccgtggagg ttcgtatgaa
     1741 gcacactgcg ctcgcgcttg ccatgctcct cgtctcggcg acggccgcgt ccgccgaggg
     1801 ccctcgtcaa ccgctcgctg ccaatgaggg cgcgcgagga tcgacggtcg tgcaaaacga
     1861 tttctgggtc agcacgcagg accgggagcg gcgccgggac gagcgccgcc gcgggatcgg
     1921 tgcaatcctg tattccaatc cggatttgcg cggcgagcgc tacatcatcg aaggcgagta
     1981 catgcgcaac ctcgggaaca ccggcttcaa cgaccgcgca caatctctgc aggtcgagcg
     2041 cggctactgg atcttctgca gcgacgccga gttccagggc acctgccgca cgttcgggcc
     2101 cggggaatac cggcggctgc cgcgcggcct cgacaatcga atctcctcgg gtcgccggat
     2161 ctcggaaaac tatccgtacc agggccgtcc gaactgggag cgccgatagc gtcatgccga
     2221 tcgtcgacga catcgccggc ctgcgtcgca tcctcgcgcg ctcgcgcacg ctggcggtcg
     2281 tcgggctttc ggcgcagtgg tatcggccga gctacttcgc cgcgaagtac atgcaggacc
     2341 acggttaccg catcatcccg gtgaacccgc gttacgacga ggtgctcggg caaaaatgct
     2401 ttcccgacct tcgctcgatt gcgcaaccgg tggacctcgt cgactgcttt cgcaagccgc
     2461 aagacatccc gccgatcgcc gaggacgcga tcgcgatcgg cgcgaaagtg ttgtggatgc
     2521 agctcggcat cgagaatcgg gacgccgcgc agcgcgccgg cgacgccggg ctcgaggtgg
     2581 tgatgaatcg ctgcgtgaag atcgagcacg cgcgcatcct cggcggcctc aactgggcag
     2641 gcgtcaatac cggcgtgatc tcgtcgcggc ggccgtcggc ttcgtagccg gcaccatgcc
     2701 tcgccggcgc gccggcgagg cttcgcctgg ggtccgtttc gcgtctcgcg cataatcgcg
     2761 aaccggcagt ggttcgccgc tcgcctaccc gggacctgcg cgcatgcccg atcacgccta
     2821 cggcttcgac acgttgtgcc tgcatgctgg gcagattccc gacgcgcaaa cgggtgcccg
     2881 cgcgatgccg atctaccaga cgacgtcgtt cgtcttcgac gatgccgacc acgcggcatc
     2941 gctcttcaac ctgcagacgt tcggcaacgt ctattcgcgc atctcgaatc cgacggtggc
     3001 agcgctcgag gaacgcgtcg cggcgctcga aggcggccgc gcggcgctcg ccgccgcgac
     3061 cggcatggcc gcgcagatgc tcacctttct cacgctggca cgcgccggcg accatatcgt
     3121 ggctgcgcgc acgctctacg gcgggtcgta ttcgcagctc gccgtgacgt tcgcgcagct
     3181 cggcatcgac tcgacgttcg tcgacgccgg cgatcctgag aactttcgcc atgcgctgaa
     3241 accgtcgacg aagtgtctct acgccgagac gatcggcaac ccgcagctca acgtgccgga
     3301 catcgccgcg ctggccgaga tcgcgcacgg cgccggcgtg ccactcgtca tcgacaacac
     3361 gctcgcatcg ccgtaccttt gccgcccgtt cgagcatggc gccgacatcg tcgtgcattc
     3421 gctcaccaag tacctcggcg gccacggcac gacgatgggc gggatcgtcg tcgaatcggg
     3481 caagtttccg tgggacaacg gcaacttccc gcagatgacc gagccgtcgc gcggctatca
     3541 cggggtgatc ttccacgaga cgttcggcga cttcggcttc acgatgaagg cgcgcatgga
     3601 gaccatgcgc acgctcgggc caacgctgtc gccactgtca gcgttcctgc tgctgcaggg
     3661 cgtcgagacg ctgcatctgc gcatgccgcg gcattgcgaa tcggcgctcg ccgtcgcgca
     3721 acatctcgag cgccacccgg ccgtcgaatg ggtcaattac ccgctccttc cgggaaatcc
     3781 gggccatgcg ctcgcgcaac gctacctgcc gcgcggcgcg ggcggcatcc tgaccttcgg
     3841 ggtcaagggc ggtgcggccg ccggcgtgcg ctgcatcgag gcggtgcaat tcctttcgca
     3901 tctcgccaat gtgggtgatg cgaaaacact tatcattcac cccgcatcga ccacccatcg
     3961 ccagttggac gaaggcgagc aacgggcggc cggcgtgtcg ccggagatga tccggctgtc
     4021 ggtcgggctc gaatcgctcg acgacatcct gtgggatctc gaccaggcgc tcgagcaatc
     4081 gcaacgctga gccaattcgc gacatcaccc cccaaaactc tcgcgccccc gagcgcgcct
     4141 gcccccgatg aatcgctacg cctccatacc gtggccgtac gcagcgctgt tcctttgcgc
     4201 cgccttgatc gcccaggaat tcttgctgcg ctcgttcggt gcgcagcccg ccatgtccga
     4261 atcggcgtat cgcgttttcg tgatgacgct cggcgtggcg tcgctcgcgc tgatcgtcct
     4321 gccgccgcgt cgcaccgcgt acctgctcgg cttcatcgtg tgcgccgcgc tgatggcgtg
     4381 ggcgctgtgg ctgcagtacg gcgagggcct cgagccatgc ccgctttgca tgttccagcg
     4441 cgtgtgcgtc tccgcggtcg gcatcgtgtt cctgatcgcc gcaatccaga atccggggag
     4501 gaggagcgct gcgttctacg cgctgctgac gctgatcatc gccggtgccg gcgccgcgtt
     4561 cgccgcgcgc cagatctggc tgcaggcgct gccgaaagac caggtgccgg cgtgcgggat
     4621 ggggttgtcg tacatgatgg acacgctgcc gttcaccgac gtggtgcgca aggtgctgga
     4681 gggcagcggc gaatgcgcgg agaaaggctg ggtgctcctc ggcctttcga tcgccggctg
     4741 gacgttcgtg ttcttcgtga gcatgatcgc cgcggccatc gcgctgatca ggcgcgatta
     4801 gtcctgtccg atgcattagt cgcgccgctg cggcggttcg ctcctcgtac cgtctggtcg
     4861 aggaggggtt gacggtctga aaacaaatcg gcccgcagtt gcgcgggccg aaacgtacgt
     4921 tgcgtcggtc atcgtgactt ccgtcgtcgg gcgaatccga tccccgacga gccgaggccg
     4981 agtggcgctc aatgcgccgt agtcagcgcg ctgcccccgg cttattccat ttatagggaa
     5041 agccaggttg tgccgagcag gccaatatcg acgatgccct ttgcccctct ggcgtattga
     5101 gcaggcgaat gatttcgcac cccagagcgc tgagcgcgga cacgatggtg tccttgttgg
     5161 cattatcgtt attgatgatc gccgtcgtgt tcgtgttggc gttgctgatg atcgaggctg
     5221 tgctctggtt gatattcgtg ttgatcgccg ccgtgctctg gttgatgttg gttgtgatcg
     5281 ctgcaacatc ggccgtgagg tcgtcatgaa tgtgagcaag ccgttccaag ttggccgtga
     5341 cctgattggc cgtcacctcg cccgcgcaaa agttggctac gtcgatcaac ccctgcgtga
     5401 tatctgccgc aattgccaga ggcacgcacg cgagcgagcc gttctcgccc aggatgtcct
     5461 cgttgcaggc ccacgccgcc gcagccagaa tcgactgtgt aacctcgttc gcaatcgtct
     5521 cggcgtaccg cgtttcgctc gtatagtcgg ttccgacaca tccgttgaca atggccggtt
     5581 cgccagggaa gggcagcgat ttttgttgct gggtcggctt gcgctccacg aggagcttgg
     5641 ccacgctggc tactgtcgaa aagtcaggaa tgcgggtctt ggcaaacacc ttggcgagct
     5701 gttcatccgt aattgcctgc attgctgcac gccccctttg cgcacgtaac tggccatcga
     5761 cattgccgct ggtcgcaaag agttgctcgt agttggccat catgatgtcg tattgcactc
     5821 ggaacgcggc gatggtcccc cgagtttggg cgataacagg cgccgcaacc actagaccga
     5881 ccaataggaa agccaaaccc aggcatctgg aagcccgatc cttatagatc ttcatctcgt
     5941 ttccccggtt tttttcttga ctcgaatgtc gcgtcttgcg gcgcggcgac cgcgctgcac
     6001 gtcggggtcc cggacacgag gacgatgtgc tgcgctagat gcttctacgc gctatccccc
     6061 aaccgtacaa gcgaattcag cgtccaccat cgttgggaaa tatgctcggc gcataaggta
     6121 tgcccattgc cataggcggg cctgctgatt tgtcgccggg gcggtcggca gagcaacccg
     6181 gacgcaacgg cgctgccgcc atgccgcaat cggagaacgt cttgagcgtc cgcctccacg
     6241 gcgcggaccg ggcgttgaac ccctaagctt ccggacgagg agaacggtct tgcttctcct
     6301 cgcgccgttt cagtcttcgg cgaatgatga ttcggctcgc aatgaatgcg tagcaaagac
     6361 caatcaacga tgcgccgaga attagcagta tcagcagccc agtcgacatg gattcaggct
     6421 aacacgaggt tacttggctt acctggcgag aatggccgag cgtgcggcgg tggcctaagc
     6481 cagcacctcc cggcgccggc ggatctgccc gatgtgctcg tcacgcatct gctcgaagcc
     6541 ctcgtcgagc gcctgctcga gtatggcagc catctgcgcc gccgaatgca gccgcgagac
     6601 gaaaacgaaa tcggcgcccg cgtcgtagag catcagcgcc gatgcggcac ggttggccgt
     6661 gacgatcacc ttcgcctgcg gccacagccg gcgcgcgagc ttcagcaggc gcaggttgtc
     6721 ggtacccttg aggatcgcat cgggaatggt cgacacgacg agcctcgcgt cgtgcacgcc
     6781 ggcgtgatgc agcgtatcca tgttcgcgac gtcgccgtac ctgcatgcga tgccgcgccg
     6841 cgtcagctcc gcgtgcacct cgggattgaa atcgatgacc agcaaccgct cgagcatcgc
     6901 cttcgcatgc gcggtctcgc ccatctcgta ctcgtgcacg agcgcgctcg cctcggtgaa
     6961 gaagccgagg agcacgacat ccttggttgt gctggcgtgc gcatcgtccg gcgctggctc
     7021 ggcgtcgtcg agatccttca cgccggcgtt gcgcagccac cgcgtcagcg ttcgctgcag
     7081 cggatggctg tagccgatca ggtacgtcga cgcgaccgac gtgaacgcga acacgaaaat
     7141 cagcaggctg gcggttttcg tgccgatatg gccgtagctc agtccgatcg ccgcgatcac
     7201 catcgaaaac tcgctcatct gcgcgagatt gatcgccggc agcaggctcg tccggtgtcc
     7261 ttgccgcaag ccgcgcagga tcgggaacac gacaacgaaa cggcttgcca cgaggaagag
     7321 cgacgcgacg agtgccagcg caatgatttc caaggtcggc atcgggatct tcatgcccag
     7381 cgccacgaag aacagggtca cgaagaaatc gcggatgctg acaacctttg cgatcacgtc
     7441 gaggttgtac ggaaacgtcg agatgctgat gcccgcgatc agcgcaccca tctcccgcga
     7501 aagaccgacg ccgctcgccg ccgcgcagac gatgaagcac catgcgagcg atccgatcag
     7561 cacgagctcg ggcagcttgg ccaccgaacg gaacagcctg ggcaataccg tcttgctgat
     7621 gagcagcgtc gcacccacca gcagcacacc gctgccgagc gacagcagca ccttgccgag
     7681 ctcggggttg cgcaggttcg gctggatgcc cagcactacg attgcccata cgtcctggaa
     7741 gatcagcacg ccgagggtga tgcgcccggg cagcgtatcg agctcgaact tgtcgtagag
     7801 gagcttgacg acgatcatcg tgctgctcaa tgcaaggcac acggcgacgt acagcagcga
     7861 gaagtcgccg ccctgcatcc tgaagccgaa cggcaggaag aacgcgaggc cgagcgcgag
     7921 gcacagcggg aactgcagga cgccggcgac gagcaccggc ttgccggccg agagcagttt
     7981 cttcaggtcg agctcgaggc cgatgatgaa gagcagcagg atgaggccga tctcggagac
     8041 ggtctcgatg ctcgcttcgt cgctgacgag accgaggccg atctgcggtc cgatcagcac
     8101 cccggcgagc agataggcga gcagcagcgg ctggcgcagc cggttgccga tgaacgcgag
     8161 gatcgccgcg accgagatcg cgatgccaac gctccccaga agatcgatgt gctgcatgct
     8221 ttcctcgata gtgcgacggc gtgacgaaag gtgccggtcc cgggcgccgc gctgccgctc
     8281 gcggttactt cgctgccatc ctgatcgcgc cgtcgaggcg gatcacttcg ccgttcagca
     8341 tcgggttggc gacgatttcg cgcacgagcg aagcgaactc ggccggcttg ccgagacgcg
     8401 gcgggaacgg caccattttg ccgagcgcct cctgcacttc gggcgtcagg ctcgccatca
     8461 tcggtgtctc gaacaggccc ggcgcgatcg tcatcacgcg aatgccggag cgcgcgagct
     8521 cgcgcgcaat gggcagcgtc atgccgacca cacccgcttt cgacgcgctg taagccgcct
     8581 gcccgatctg gccgtcgaat gcggcgacgg aagcggtgtt gatgatcacg ccgcgctcgc
     8641 cggtgtcgtt cggcgcgttc ttcgccatcg cctgcgcggt gagccggatc aggttgaacg
     8701 tgcccacgag gttgatgttc accgcgcgcg caaaggtggc gagcgaatgc gcgccctcct
     8761 tgccgacgac cttctcgccg tgcacgatgc cggcgcagtt gacgagcgcg tggatcgcgc
     8821 cgaatgcctt cagggcagcg gcgatggccg cctcggcgct cgcttcgtcg gtgacgtcgg
     8881 tgcggacgaa ccgtgtggca tcgcccaggt cgccggccag cgccttgcct cgcgtttcgt
     8941 cgagatcggc gatgacggcg tgaccgccac cgtcgaccgc catccgaacc gtggccgcgc
     9001 cgaggcccga cgcaccgccg gtgacgagga acgtgctgcc gcgcaattgc atgctgatga
     9061 cgctccttgc taagcggttg cgctcgggac ggtcttcacc accgacagcg cggtggcgat
     9121 gagccctgcg acgtcggcga cattcgccgg caggatcacc gtgttcgtcg ccttcgcaag
     9181 attgccgaac tgcgcgatgt attcctcggc gaccttgagg ttcaccgcgc tcgcgccgcc
     9241 cggcgccgcg atcgcatcgg ccacctggcg gatcgcggtc gcgttggctt cggcgagcgc
     9301 gagaatcgcc gccgcctggc cctgcgcgtt gttgatctcg gcctgcttct cgccttccga
     9361 cttcgcgatc gccgcctcgc gcgcacccta cgcgatgttg atctgctcct gccgcttgcc
     9421 ttccgacgtg gcgatcaccg cgcgcttctc gcgctcggcg gtgatctgcg cctgcatcgc
     9481 gcgcaggatc tccgccggcg gcgtcaggtc cttgatttcg tagcgcagca ccttcacgcc
     9541 ccagttcggc gcggccgcgt cgagcgcgct gacgactgcc gcgttgatct ggtcgcgctc
     9601 ctcgaacgtg cggtccagct ccatgcggcc gatcacgctg cgcagcgtgg tctgcgcgag
     9661 ttgcgtgatc gcgagcacgt aattggtcga gccatacgac gcgagccgtg catcggtcac
     9721 ctggaa
//
LOCUS       DASSVX010000166         6906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_156516, whole genome shotgun sequence.
ACCESSION   DASSVX010000166 DASSVX010000000
VERSION     DASSVX010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156516"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..156)
                     /locus_tag="VFO53_07050"
                     /pseudo
     CDS             complement(1..156)
                     /locus_tag="VFO53_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            243..476
                     /locus_tag="VFO53_07055"
     CDS             243..476
                     /locus_tag="VFO53_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876069.1"
                     /translation="MRVWLVSGDPAPPAALLAEHPDLTAVRVAAPPALPRGAARIYLV
                     DPLGNFVLAWPSKPDIKAMARDLGRLLRASSIG"
     gene            500..1417
                     /gene="cyoE"
                     /locus_tag="VFO53_07060"
     CDS             500..1417
                     /gene="cyoE"
                     /locus_tag="VFO53_07060"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET8876070.1"
                     /translation="MADFEGSPPLTTLAFAESRARQLFALTKPRVVSLIVFTAVIGMF
                     LAVPGVPPAAAVFFGTIGIALVAGAAAAINCLVEQNIDKLMRRTSWRPLPRGQLTSRD
                     TLLFALGVGSIGLWILYRFVNPLTMWLTLATFVGYAVIYTVLLKPATPQNIVIGGASG
                     AMPPVLGWAAVTGSVPPEALLLFLIIFVWTPPHFWALALYRRDDYANAGLPMLPVTHG
                     IRYTQVMIVLYTVALTAVTLLPYALKMSGLPYLVAAGVLGGVFTGYAVRLYRRYSDRL
                     ARAMFRFSIVYLAALFSALLVDHYWLRIA"
     gene            1449..2057
                     /locus_tag="VFO53_07065"
     CDS             1449..2057
                     /locus_tag="VFO53_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET8876071.1"
                     /translation="MRAPSRHASGRRQCLIVLASLALAACGPSGPKFESSDITGSSFG
                     HDFSLDDPDGKPRTLADFRGKAVVVFFGYTQCPDVCPATLATLADAMKQLGADADRVQ
                     VLFITIDPERDTPGLLKQYVPAFDRRFLGLYGDAAATARTAKEFKVIYQKVAGPTPGS
                     YTMDHSAGSYVFDPKGRLRLFVSNGQGAGVFAHDLRELLDGA"
     gene            complement(2213..2596)
                     /gene="gloA"
                     /locus_tag="VFO53_07070"
     CDS             complement(2213..2596)
                     /gene="gloA"
                     /locus_tag="VFO53_07070"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012367981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HET8876072.1"
                     /translation="MRILHTMLRVGDLERSIDFYTNVLGMRVLRRTDRPDQRYTLAFV
                     GYGDERSNAVLELTFNYGVSSYDVGTGYGHVAIAVPDAAAACAAVREKGGNVTREAGP
                     VKGGTTVIAFVQDPDGYKIELIEHP"
     gene            complement(2627..2791)
                     /locus_tag="VFO53_07075"
     CDS             complement(2627..2791)
                     /locus_tag="VFO53_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HET8876073.1"
                     /translation="MKKYMCLICGWIYDEAAGSPEDGIAPGTRWEDVPPNWTCPECGA
                     RKEDFEMVEF"
     gene            2929..3834
                     /gene="thiD"
                     /locus_tag="VFO53_07080"
     CDS             2929..3834
                     /gene="thiD"
                     /locus_tag="VFO53_07080"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET8876074.1"
                     /translation="MPHDADPAFPPLVLTFSASDPSGGAGMQADLLTLSSMGCHPLSV
                     ITALTVQDTLGIEGVLAIDADWVADQARALLEDMPVDAFKIGVLGSVENIAAIAEIVS
                     DYPDVPLVLDPVLASGRGDELATDEMAQAMRELLLPQTTILTPNSMEARRLADIDDED
                     EPSLFTCATRLVEAGSEYVLVTGTHEATAQVVNTLYGKSGVVRTDTWPRLPHKYHGSG
                     CTLASAIAAMLANGLELPEAVREAQDYTWHALEKAYRPGMGQHLPDRLFWAREERDAH
                     ADESAEAVANEASETHRHADARGTH"
     gene            3870..4481
                     /gene="thiE"
                     /locus_tag="VFO53_07085"
     CDS             3870..4481
                     /gene="thiE"
                     /locus_tag="VFO53_07085"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET8876075.1"
                     /translation="MTPGEADTDLLLAKVVAAIGGGAAIVQYRDKALAPGVRRAQARL
                     LACAPAVRRALFIVNDDPDLARDVDADGVHLGEDDAPIAAARARVGPERLVGVSCYDD
                     FERARAAVDAGADYVAFGSFFASPVKPDARRASVALLTRARALGVPVVAIGGITAANA
                     ATLVDAGASAVAVISDVFAHADPADVTRAAMAIAHCFRARQSR"
     gene            4478..5932
                     /gene="hemL"
                     /locus_tag="VFO53_07090"
     CDS             4478..5932
                     /gene="hemL"
                     /locus_tag="VFO53_07090"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET8876076.1"
                     /translation="MSTAEPPQGANSAPAGGSAAAKPQAWGEQTSAAGPSQGANSAPA
                     GGSAAASAASVGGCASRNEELFARAQRSIPAGVNSPVRAFRSVGGIPRFIVRGQGAYV
                     FDADGRRYIDYVGSWGPAIAGHAHPAVVSAVQAAAANGLSFGAPTEVEVELAEALIAR
                     LPSLDLVRLVSSGTEATMSALRLARGYTGRSKIVKFEGCYHGHGDSLLVKAGSGALTF
                     GQPSSAGVPASIANETIVLPYNDVAAVNETFAADGDAIAAIIVEPVAGNMNLVLPTPG
                     FLEGLRTVCDRFHAVLIFDEVMTGFRVHSRGAQGRYGITPDLTTLGKVIGGGMPVGAF
                     GGRRAIMEQIAPLGPVYQAGTLSGNPVAVAAGLATLALTGAPGFYDALEQKTRSLADG
                     LVRAAKRHDVAFAAQALGGMFGIYFAAAVPASYSDVMACDTARFNRFFHAMLDAGIYL
                     APSAYEAAFVSAAHGNAEIDETLAAADRAFAAMR"
     gene            6068..>6906
                     /locus_tag="VFO53_07095"
     CDS             6068..>6906
                     /locus_tag="VFO53_07095"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET8876077.1"
                     /translation="MDAPARKRIGELLIERGKLDQANLERALRLQSETGEKLGALLVT
                     LGLCAQRDVAEALALQLALPLVDANGYPEFPILEERVSARFLREARVLPVREDEAELG
                     LAMADPTDAYTISAFEMVTGRAVRPMVAIPTDLEAALERLYGAGRSAVGQLVGDVETR
                     TDELSFDADVQQLKDLASEAPVIRLVSLLITNALEMRASDIHIEPFENRLIVRYRIDG
                     VLHEVESPPRRLSAAVISRIKIMANLDIAERRLPQDGRIELNVGGNPVDLRVSVLPTM
                     FGE"
BASE COUNT          995 a         2403 c         2357 g         1151 t
ORIGIN      
        1 atcgcggcgc gccttgggat cgacgcccgc agtcggctcg tcgagcagca gcagctgcgg
       61 ctcgtgcagc atgcaggcgg cgagtgcgag ccgctgcttc catccgccgg acagcgtgcc
      121 ggcgagctgg cgccgccgcg cagagaggcc gaggcgtgcg agcgtgtcgt cgatcgtgcg
      181 ccgacgcact gtatgcgtcg cgccaggcgc gcacgatcca gaatgccgag cgcgagcgcg
      241 tggtgcgcgt gtggctcgtc agcggcgacc ccgcgccgcc tgcagcgctc cttgccgaac
      301 accccgatct caccgcggtg cgcgtcgcgg cacctccggc gctgccgcgc ggcgccgcgc
      361 gcatctatct tgtcgatccg ctcggcaact tcgtgcttgc ctggccgtcg aagcctgaca
      421 tcaaggcgat ggcccgcgat ctcggccgcc ttctgcgcgc gtcgagcatt ggctaggggc
      481 cacggatcca cgctgtaaaa tggcggactt tgaaggctct cctcccttga ccaccctcgc
      541 gttcgccgaa tcccgcgccc ggcagctttt cgcgctcacg aaaccgcgcg tcgtctcgct
      601 gatcgtgttc acggcggtca tcggcatgtt cctcgcggtc cccggcgttc cgcccgcggc
      661 agcggtgttc ttcggcacga tcggcatcgc gctcgtcgcg ggcgccgcgg cggcgatcaa
      721 ctgcctcgtc gagcagaaca tcgacaagct gatgcgccgc accagctggc ggccgctgcc
      781 gcgcgggcag ctcacctcgc gcgacacgct gctgttcgcg ctcggcgtcg ggtcgatcgg
      841 cctgtggatc ctctaccgct tcgtcaaccc gctgacgatg tggctcacgc tggccacgtt
      901 cgtcggctac gccgtcatct acaccgtgct gctgaagccc gccacgccgc agaacatcgt
      961 gatcggcggc gcatcgggtg cgatgccgcc cgtcctcggc tgggccgcgg tgaccggcag
     1021 cgtgccgccc gaggcgctgc tgctgttcct gatcatcttc gtctggacgc cgccgcattt
     1081 ctgggcgctg gcgctctatc ggcgcgacga ctacgcgaat gcagggcttc ccatgctgcc
     1141 ggtgacgcac ggcatccgct acacgcaggt gatgatcgtg ctctacaccg ttgcgctcac
     1201 ggcggtcacg ctgctgcctt atgcgctgaa gatgagcggg ctgccgtatc tcgtggcggc
     1261 gggcgttctc ggcggcgtgt tcaccggcta cgcggtgcgc ctctatcgcc ggtacagcga
     1321 tcggctggcg cgcgccatgt tccgcttctc gatcgtctac ctcgccgcgc tcttttcggc
     1381 gctcctcgtc gatcactact ggctgcgcat tgcgtgacat ggctgcgtcg attttcgcgc
     1441 tgccggtgat gcgagcgccg tctcgtcacg cgagcggccg ccggcaatgc ctgatcgtac
     1501 tggcgtcgct cgcgctcgct gcatgcgggc cgagcgggcc gaagttcgag tcgtcggata
     1561 tcaccggctc gtcgttcggt cacgacttct cgctcgacga tcccgacggc aagccgcgca
     1621 cgctcgccga tttccgtggc aaggccgtcg tggtcttctt cggttacacg cagtgccccg
     1681 acgtgtgccc ggcgacgctg gcgacgctcg ccgacgcgat gaaacagctc ggcgccgacg
     1741 ccgatcgcgt gcaggtgctg ttcatcacca tcgatcccga gcgcgacacg cccgggcttc
     1801 tgaagcaata cgtgccggcg ttcgaccggc gcttcctcgg cctgtacggt gacgccgctg
     1861 caacggcgcg cacggcgaag gaattcaagg tcatctacca gaaggtggcg ggcccgaccc
     1921 ccggcagcta cacgatggat cactcggccg gcagctacgt tttcgatccc aaggggcggc
     1981 tgcgactgtt cgtgtcgaac gggcaggggg ccggagtgtt cgcgcacgac ctgcgcgagc
     2041 tcctcgacgg agcgtaggtg gtccccccac gcttgcggcc gattcctcgg ctctcgtgct
     2101 ttgcactcga ctgttcgccc tcggggcggc ccatcgggct cacgcgctgc gctgcccccg
     2161 gagggggcgc aatgcgcgcc ttgaggcggc tcggcgtcgc gcatgggccg cgtcacggat
     2221 gttcgatcag ctcgatcttg tagccgtcgg gatcctgcac gaacgcgatc accgtcgtgc
     2281 cgcccttcac gggccccgcc tcgcgcgtga cgttgccgcc cttctcgcgt accgctgcgc
     2341 aggccgcggc cgcatcggga acggcgatcg cgacgtggcc gtatccggtg ccgacgtcgt
     2401 agctcgaaac gccgtagttg aaggtgagct cgagcacggc attcgaccgc tcgtcgccgt
     2461 aaccgacgaa cgcgagcgtg tagcgctggt cgggccggtc ggttctgcgc agcacgcgca
     2521 tgccgaggac gttcgtatag aaatcgatcg accgctcgag gtcgccaacg cgaagcatcg
     2581 tgtgcaggat gcgcatgccg tgttttcctg gtgattggag agtcgcctag aactcgacca
     2641 tctcgaaatc ttccttgcgt gcgccgcact cggggcaggt ccagttcggc ggcacgtctt
     2701 cccagcgcgt gccgggtgca atcccatcct cgggcgatcc ggcggcctcg tcgtagatcc
     2761 agccgcagat caggcacatg tattttttca ttgcttgaag cgggcctcct gtgcgcgtca
     2821 aagcgagcgc tggaccgcgc cccggcgagt gatgtgcgtg gggtagaatg cgaagtgtag
     2881 caaccccgcc caacgccgcg tcgcgctctg caaagccgac gcgccgccat gccccacgac
     2941 gccgatcccg cttttccgcc gctcgtcctc acgttttccg cctccgatcc gtcgggcggc
     3001 gccggcatgc aggccgatct gctcacgctt tccagcatgg gctgccatcc gttgtcggtc
     3061 atcaccgcgc tgaccgtgca ggacacgctc ggcatcgaag gcgtgctggc gatcgacgcc
     3121 gactgggtgg ccgaccaggc gcgcgcgctg ctcgaggaca tgcccgtcga cgcgttcaag
     3181 atcggcgtgc tcggcagcgt cgagaacatc gcggcgatcg cggagatcgt gtccgattat
     3241 cccgacgtgc cgctcgtgct cgaccccgtg ctcgcttccg ggcgcggcga cgagctcgcc
     3301 accgacgaga tggcacaggc gatgcgcgag ctcctgttgc cgcagacgac gatcctcacg
     3361 ccgaacagca tggaagcgcg ccggctcgcc gacatcgacg acgaggacga accgtcgctt
     3421 ttcacctgcg cgacgaggct cgtcgaggcg ggcagcgagt acgtgctcgt caccggtacg
     3481 cacgaggcga cggcgcaggt cgtgaacacg ctctacggca agagcggcgt cgtgcgcacc
     3541 gacacctggc cgcgattgcc gcacaagtat cacggctccg gctgcacgct ggcctcggcc
     3601 atcgccgcga tgctcgcgaa cgggctcgag ctgcccgaag cggtgcgcga ggcgcaggac
     3661 tacacgtggc atgcgctcga gaaggcatac cggcccggca tgggccagca tctgccggat
     3721 cggctcttct gggcgcgcga ggagcgcgac gcgcacgccg acgaatccgc cgaggcagtc
     3781 gcgaacgaag cgagcgagac gcaccggcat gccgatgcgc gcggaacgca ttagcggcgc
     3841 gcgcgtcgcg ggttgccggc ctttatgcgg tgacgccggg ggaagccgac accgacctgc
     3901 tgctcgcgaa ggtcgtcgcc gcgatcggcg gcggtgcggc aatcgtccag tatcgcgaca
     3961 aggcgcttgc tcccggcgtg cggcgggcgc aggcgcgatt gctcgcgtgc gcaccggccg
     4021 tgcgtcgggc gctgttcatc gtcaacgacg atcccgacct cgcgcgcgac gtcgacgccg
     4081 atggcgtgca cctcggcgag gacgacgcgc cgatcgccgc cgcccgcgca cgcgtcggcc
     4141 ccgagcggct ggtcggggtg tcgtgctacg acgatttcga acgcgcgcgc gccgcggtcg
     4201 atgcaggcgc cgactacgtc gcattcggca gtttcttcgc gtcgccggtc aagcccgacg
     4261 cacgccgtgc gagcgttgcg ttgctcacgc gggcgcgtgc actcggcgtt ccggtcgtcg
     4321 cgatcggcgg catcaccgcc gcgaacgcgg ccacgctggt cgatgccggc gcaagcgcgg
     4381 tcgccgtgat cagcgacgtg ttcgcgcacg ccgacccggc cgacgtgacg cgcgcggcga
     4441 tggccatcgc gcattgcttt cgtgcacgcc agagccgatg agcaccgcag agccgcctca
     4501 aggtgcgaat tccgcccccg cggggggcag cgcagcggcg aagccgcaag cgtgggggga
     4561 acaaacaagc gccgcagggc cgtcccaagg cgcgaattcc gcccccgcgg ggggcagcgc
     4621 cgcggcgtca gccgcaagcg tggggggatg tgccagtcga aacgaagagc tctttgcgcg
     4681 cgcgcagcgg tccataccgg ccggcgtcaa ttcgcccgtg cgtgcgtttc gctcggtcgg
     4741 cggcatcccg cgcttcatcg ttcgcggcca gggcgcgtac gtcttcgatg ccgacggcag
     4801 gcgctacatc gactacgtcg gctcttgggg tcccgcaatc gccggccacg cgcacccggc
     4861 ggtggtgtcg gccgtgcaag cggcggccgc caacggactg tcgttcggcg cgccgaccga
     4921 ggtcgaggtc gagcttgccg aggcactgat cgcgcgtctg ccgagcctcg atctcgtgcg
     4981 gctcgtgtcg tcgggaaccg aggccacgat gtcggcgctg cgtcttgcgc gcggctacac
     5041 gggccgctcg aagatcgtca agttcgaagg ctgctatcac ggacacggcg acagcctgct
     5101 cgtcaaggcc ggctccggtg cgctcacctt cgggcagccg tcgtcggcgg gcgtgcccgc
     5161 atcgatcgcg aacgagacga tcgtgctgcc gtacaacgac gtcgccgcgg tgaacgaaac
     5221 gttcgcggcc gatggcgacg cgatcgccgc catcatcgtc gagcccgttg ccgggaacat
     5281 gaacctcgtg ttgcccacgc cgggatttct cgagggcctg cgcaccgtct gcgaccgctt
     5341 tcacgcggtg ctgatcttcg acgaagtgat gaccggcttt cgcgtgcatt cgcgcggtgc
     5401 gcagggacgc tacggcatta cgcccgacct gacgacgctc ggcaaggtga tcggaggcgg
     5461 catgccggtc ggcgcattcg gcggcaggcg ggcgatcatg gagcagatcg caccgctcgg
     5521 ccccgtgtat caggcgggga cgctgtcggg caatccggtg gcggtcgccg cggggctcgc
     5581 gacgctcgcg ctgaccgggg cgccggggtt ctacgacgcg ctcgagcaga agacgcgctc
     5641 gctcgccgat ggcctcgtgc gcgcggcgaa gcgccacgac gtggcgttcg cagcgcaggc
     5701 gctcggcggc atgttcggca tctacttcgc cgcggcggtg cccgcgtcgt acagcgatgt
     5761 catggcgtgc gacacggcgc gcttcaaccg gtttttccat gcgatgctcg atgcaggcat
     5821 ctacctcgca ccttccgcgt acgaagcggc attcgtatcg gcggcgcacg gcaatgccga
     5881 gatcgacgaa acgcttgcag cggccgatcg cgcgttcgcc gcgatgcgtt gacccgaagc
     5941 gtcgccgcgc ggcgacgtct tcgcgtgcaa ctccttgccg ggcaaggctt ttttgtcgat
     6001 ctctccgcat ggctacgtgc tatgatgcgc ggcgcaatcg acagatctcc aacctctgat
     6061 tcctgaagtg gacgcacccg cccgaaaacg aatcggcgag ctcctgatcg agcggggcaa
     6121 gctcgaccag gcgaacctcg agcgcgcgct gcggctgcag tcggaaacgg gtgaaaagct
     6181 cggcgcgctc ctcgtcacgc tcgggctttg cgcgcaacgc gatgtcgccg aggcgctcgc
     6241 attgcaactc gcgctgccgc tcgtcgacgc gaacggctat cccgaattcc cgatcctcga
     6301 ggagcgcgtg tcggcgcggt tcctccgcga ggcacgcgtg cttccggtgc gcgaggacga
     6361 ggccgagctt gggctcgcga tggccgatcc caccgacgca tacacgatca gcgcgttcga
     6421 aatggtgacg ggccgtgcgg tgcggccgat ggtcgcgatc ccgaccgatc tcgaggccgc
     6481 gctcgagcgc ctgtacggcg cgggccgctc ggccgtcgga cagctcgtcg gcgacgtcga
     6541 gacgcgcacc gacgagctct cgttcgacgc cgacgtgcag cagctgaagg acctcgcttc
     6601 cgaggcgccg gtgatcaggc tcgtgtcgct gctcatcacc aatgccctcg agatgcgcgc
     6661 gtcggacatc cacatcgagc cgttcgagaa ccggctgatc gtgcgctacc gcatcgacgg
     6721 cgtgctgcac gaagtggaat cgccgccgcg gcggctgtcg gccgccgtca tctcgcgcat
     6781 caagatcatg gcgaacctcg acatcgccga gcgccggctg ccgcaggacg gccgcatcga
     6841 gctgaacgtc ggcggcaacc cggtggacct gcgggtgagc gtgctgccga cgatgttcgg
     6901 cgagag
//
LOCUS       DASSVX010000167         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_456401, whole genome shotgun sequence.
ACCESSION   DASSVX010000167 DASSVX010000000
VERSION     DASSVX010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456401"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="VFO53_07100"
     CDS             complement(<1..1375)
                     /locus_tag="VFO53_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876078.1"
                     /translation="MSETSGAVAEAASASGPWNPGIQSEIPDAIRHLSTFLRPENVYT
                     SEAGARELRDLTGLDLADVVAFRPQRLALHELLIRVTADLCVPDGVRIEDLGINFRRI
                     TRTVLVNHIVPRMASVSAEYARVRRDLSARIEREIEALFGASAVAPARKRLGFAFLAR
                     RTTRDDADAPGTRESRVAAQWDAKAHACADPLEAAACRALARVLFALLVRHGRAWGSR
                     EMIASLALDIACNECASDAIGLLIEPWLANAVHAEDYRRLPRQERPVVMNTKGASASG
                     KSTLRPLQRRLASEIGVDWREFALISPDIWRKQLLDYSSLGAHYKYAGAFTGEELKIV
                     DQKLDRYMARKGARGEMPHLLIDRFRFDSFAPDSDEAGSNLLTRFGQILYLFFMITPP
                     ASLVERAWNRGLEVGRYKAVDDTLAHAVEAYSGMPDLLFTWIDRRDKDVHFEFLDNSV
                     PNGERPRT"
     gene            1412..>2985
                     /gene="lepA"
                     /locus_tag="VFO53_07105"
     CDS             1412..>2985
                     /gene="lepA"
                     /locus_tag="VFO53_07105"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET8876079.1"
                     /translation="MQNIRNFSIIAHIDHGKSTLADRFIQRCGGLADREMEAQVLDTM
                     DLERERGITIKAQTAALAYRARNGETYQLNLIDTPGHVDFAYEVSRSLAACEGALLVV
                     DASQGVEAQTVANCYTAIEQGVEVVPVLNKIDLPSAEPERVIDEIEDIIGIEADDALR
                     ASAKTGEGIDDILEAVIARIPPPAGDASAPLQALLIDAWFDNYVGVVMLVRVMQGTLK
                     PRERILLMASGATFTCEQVGVFTPRSVAREALAAGEVGFVIAGIKELKAAQVGDTITS
                     VGRPARSALPGFKEVKPQVFAGLYPVESNQYEALRNALDKLSLNDASLRYEPEVSQAL
                     GFGFRCGFLGLLHMDIVQERLEREYDMDLITTAPTVVYQVLLRDGTLFEIENPSKLPE
                     LSKVEEIREPVITATILMPQDYVGPVLTLCTEKRGVQKNMQYVGRQVLLTYELPLAEV
                     VMDFFDKLKSVSRGYASLDYEFKEFRAADVVKLDILINGERVDALSVMVHRSVATYRG
                     REVAAKMRSLIPRQMF"
BASE COUNT          505 a          980 c         1015 g          485 t
ORIGIN      
        1 ccgtgcgcgg acgctcgccg ttcggcacgc tgttgtcgag gaactcgaag tggacgtcct
       61 tgtcgcgccg gtcgatccag gtgaataaca ggtcgggcat tccggagtag gcctcgaccg
      121 cgtgggcgag cgtgtcgtcg accgccttgt agcggccgac ctcgaggccg cggttccacg
      181 cgcgctcgac cagcgacgcc ggcggcgtga tcatgaagaa caggtagagg atctggccga
      241 agcgcgtgag caggttgctg ccggcttcgt cggaatcggg cgcgaagctg tcgaagcgga
      301 agcggtcgat caaaagatgc ggcatctccc cgcgcgcgcc cttgcgtgcc atgtagcgat
      361 cgagcttctg gtcgacgatt ttcagttcct cgccggtgaa tgcgccagcg tacttgtagt
      421 gcgcgccgag cgacgagtaa tcgagcaact gcttgcgcca gatgtcgggg ctgatcagcg
      481 cgaactcgcg ccagtcgacg ccgatctcgc tggcaagacg ccgctgcagc gggcgcagcg
      541 tgctcttgcc cgacgccgac gcgcccttgg tgttcatcac gacggggcgt tcctgccgtg
      601 gcaggcgccg atagtcctcg gcatgcacgg cattcgcgag ccacggctcg atcaggaggc
      661 cgatcgcgtc gctggcgcat tcgttgcacg cgatgtccag cgcgaggctg gcgatcatct
      721 cgcggctgcc ccatgcgcgg ccgtggcgca cgagcaacgc gaacaggacg cgtgcaagcg
      781 cacggcaggc cgccgcctca agcggatcgg cgcacgcgtg cgccttcgcg tcccattgcg
      841 ccgcaacgcg cgattcgcgg gtgccgggcg catccgcatc gtcgcgtgtc gtgcgtcgcg
      901 cgaggaacgc gaagccgagg cgctttcgcg ccggggcaac cgccgacgcg ccgaacagcg
      961 cctcgatctc gcgctcgatc cgtgccgaaa ggtcgcggcg cacgcgcgcg tactcggcac
     1021 tgacggacgc cattcgcggc acgatgtgat tcacgagcac cgttcgcgtg atgcgccgga
     1081 aattgatgcc gaggtcctcg atgcgcacgc cgtcggggac gcacaggtcg gcggttacac
     1141 ggatcaaaag ctcgtgcagg gcaagccgct gcggacgaaa cgcgacgacg tccgcgagat
     1201 cgaggccggt cagatcgcga agctcgcgcg cgccggcctc gctcgtgtat acgttctcgg
     1261 gccgcaggaa cgtcgacagg tgcctgatcg catcgggaat ctccgactgg atgcccgggt
     1321 tccatggccc cgatgccgac gcggcctcgg caacggcgcc gctcgtttcg ctcatcccgg
     1381 tatcgttgtc ctctttccgc taaaattcaa catgcagaac atccgcaact tctcgatcat
     1441 cgcgcacatc gaccacggca agtcgacgct tgccgaccgg ttcatccagc gctgcggcgg
     1501 tctcgccgat cgggaaatgg aggcgcaggt cctcgacacg atggacctcg agcgcgagcg
     1561 cggcatcacg atcaaggcgc agaccgcagc gctcgcgtac cgcgcccgca acggcgaaac
     1621 ctaccagctc aacctgatcg acaccccggg ccacgtcgac ttcgcctacg aggtgtcgcg
     1681 ctcgcttgcc gcatgcgaag gcgcgctgct cgtcgtcgac gcatcgcagg gcgtcgaggc
     1741 gcagacggtc gccaactgct atacggccat cgagcagggc gtcgaggtcg tgccggtgct
     1801 gaacaagatc gacctgccgt cggccgagcc cgagcgggtc atcgacgaga tcgaggacat
     1861 catcggcatc gaggccgacg acgcgctgcg cgccagtgca aagaccggcg agggcatcga
     1921 cgacatcctg gaagcggtga tcgcgcgcat tccgccgccg gcgggagacg cgtcggcgcc
     1981 gctgcaggcg ctgctgatcg acgcgtggtt cgacaactac gtgggcgtcg tgatgctcgt
     2041 gcgcgtgatg caggggacgc tgaagccgcg cgagcgcatc ctgctcatgg cctccggcgc
     2101 gacgttcacc tgcgagcagg tcggcgtgtt cacgccacgc tcggttgcgc gtgaagcgct
     2161 ggcggcgggc gaggtgggct tcgtcatcgc cggcatcaag gagctcaagg ccgcgcaggt
     2221 cggcgatacg atcacgagcg tcgggcgtcc ggcaagatcc gcgctgccgg gcttcaagga
     2281 agtgaagccg caggtgttcg ctggcctcta cccggtcgag tcgaaccagt acgaggcgct
     2341 gcgcaacgcg ctcgacaagc tgtcgctcaa cgatgcgtcg ctgcgctacg agcccgaggt
     2401 gtcgcaggcg ctcggcttcg gcttccgctg cggctttctc ggcctgctgc acatggacat
     2461 cgtgcaggag cggctcgagc gcgaatacga catggacctg atcacgacgg cgcccaccgt
     2521 cgtctaccag gtgctgctgc gcgacggcac gctgttcgaa atcgagaatc cgtcgaagct
     2581 ccccgagcta tccaaggtgg aggagatccg cgagccggtc atcacggcga cgatcctcat
     2641 gccgcaggac tacgtcggtc cggtgctgac gctttgcacc gaaaagcgcg gggtgcagaa
     2701 gaacatgcag tacgtcggac gccaggtgct gctcacctac gagctgccgc tcgccgaagt
     2761 ggtgatggac ttcttcgaca agctgaagtc ggtctcgcgt ggctacgcgt cgctcgacta
     2821 cgagttcaag gagttccgcg cggccgacgt cgtcaagctc gacatcctga tcaacggcga
     2881 gcgcgtcgac gcgctgtcgg tgatggtgca ccggtcggtc gcgacctacc gcggccgcga
     2941 ggttgccgcg aagatgcgct cgctgattcc gcggcagatg ttcga
//
LOCUS       DASSVX010000168        10522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_156614, whole genome shotgun sequence.
ACCESSION   DASSVX010000168 DASSVX010000000
VERSION     DASSVX010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 10522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10522
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156614"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /gene="glyS"
                     /locus_tag="VFO53_07110"
     CDS             <1..1749
                     /gene="glyS"
                     /locus_tag="VFO53_07110"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET8876080.1"
                     /translation="KADYVYLRRTVPGMALERALQEALDVVLARLPIPKVMRYAAATS
                     YYTDVAFVRPAHRLVALHGSDVVPVRALGLDAGRMSSGHRFLARADLSIATADAYAPT
                     LEAEGKVLPSFATRRAAIARALEACAGAARVIMPDALLDEVTALVEWPAVHEGAFDAA
                     FLDVPQECLILTMQQNQKYFALADARGALVPRFLLVSNLDTADASAIVRGNERVLRAR
                     LADAKFFYDQDRKVALAARVERLAAIVYHNKLGTLRDRVERLRFLARDIGPHVGGDLA
                     LCDRATLLAKADLVTDMVGEFPELQGTMGRYYAQHDGEPAPVADAIAQHYWPRYAGDA
                     LPQSPVAQAVALADKLEALAGLFGIGAVPTGDKDPFGLRRAAIGVLRILIEKRIAVPL
                     AHLVDIAFAAFDAVPAVKPVPRELADFLYERLRSHLREQGYTANQVEAVLAQRPQRID
                     LVPDRLAAVKAFESLPESSALSAANKRIVNILRKSGTDAAPSVAVERLTDGAERDLWN
                     VFERLSPDVERHCESGDYASALKSLAAAKPAVDRFFDDVMVMVDDAGVRANRLALLDR
                     VARTMNRVADISRLAA"
     gene            1779..2360
                     /gene="gmhB"
                     /locus_tag="VFO53_07115"
     CDS             1779..2360
                     /gene="gmhB"
                     /locus_tag="VFO53_07115"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HET8876081.1"
                     /translation="MAPLASEGIVQSRVTRLIVLDRDGVINHDSDQFIKSPDEWRPIP
                     GSLEAIARLNHAGYRVVVATNQSGVGRGLLDFSTLSAIHDKMHRALAHVGGRVDAIFY
                     CPHTADSRCECRKPKAGMLREIGVRFNVDMTGVACVGDGLRDLQAAQTVGAQPMLVLT
                     GKGEKTLREGGFPGNTVIFPDLAFVASALLVAD"
     gene            2365..3096
                     /locus_tag="VFO53_07120"
     CDS             2365..3096
                     /locus_tag="VFO53_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET8876082.1"
                     /translation="METAPRATSALRSVAFALFQMLVTPPYALAVVALFWLPPVPRYR
                     FITHWCALNLWGARVLCGIRHRVIGIENVGDAPKIVACKHSSTWETLFLSRLLPPLAY
                     VAKKELLSVPFFGWGFRLASPITIDRDAGQDAMTQIATQGRERFAQGFWIILYPEGTR
                     IPAGKRVRYKTGAARLAIEMRTPILPIAHNAGWLWPKGVMGKRPGTITLSIGKPIDPG
                     TMDPARLMQTVERWIEAEVARLGEA"
     gene            3093..3845
                     /locus_tag="VFO53_07125"
     CDS             3093..3845
                     /locus_tag="VFO53_07125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HET8876083.1"
                     /translation="MKLEPLVADDPRIANGTLRRVVLAGQAVDYRLIRARRRSIGMEV
                     HFEGLTVRAPRWVTLRDIEAALVERAAWILRALAEWRARRRDVMPRTWVSGATIAFRG
                     AALTLALYPARQPAVDADLFHVTVRHPNAHDEHQVADCVRAWLRDQATTLVAARVAHY
                     VQRVGTHMPSVRLSNARSEWGSCNAKGEIRLNWRLVQLPPPLAEYVVAHEVAHIVELN
                     HSRRFWALVEALLPGHSALREQLDDWTALLAA"
     gene            complement(3855..4196)
                     /gene="cyaY"
                     /locus_tag="VFO53_07130"
     CDS             complement(3855..4196)
                     /gene="cyaY"
                     /locus_tag="VFO53_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HET8876084.1"
                     /translation="MDESAFLAAADATLERIGLALDAALETSDAELDWSLNDGVLTIE
                     CDDESRIIVNRHLPNREIWVAAKSGGYHFRAQDGRWRDVRGSDELAAALVRLLKAQAR
                     IAVRMPELPAS"
     gene            4221..4436
                     /locus_tag="VFO53_07135"
     CDS             4221..4436
                     /locus_tag="VFO53_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HET8876085.1"
                     /translation="MPRQSIHARALVAATLAVLVAIVSACGIKGPLRPAPKTTPATAT
                     PPATANPATPPATTSPALPPPSSPPPK"
     gene            4433..5698
                     /gene="lysA"
                     /locus_tag="VFO53_07140"
     CDS             4433..5698
                     /gene="lysA"
                     /locus_tag="VFO53_07140"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET8876086.1"
                     /translation="MTAAHGAAGFDYAGGELCAERVPLSALAQRYGTPCYVYSRALLA
                     RAFHEFDAAFGGVPHLVCYAMKANPSLAIVDLFASLGSGFDIVSGGELARVQAAGGDA
                     GKVVFSGVAKSDAEMEAALAAGILCFNVESAAELDHLAAVAARRGVRAPVSFRVNPDV
                     GPRTHRYIATGLKESKFGVAFDEAHALYRRAAAMAPIRVRGVDMHIGSQITELAPYRE
                     ALARVLGLVDALAADGIALEHIDVGGGLGVRYRDETPVDVGDYAAMVGELMRGRRELL
                     LLEPGRRLVADAGVLLTRVRVLKAGGARNFAIVDAAMNDLLRPSLYGAWHGVDAVRPR
                     QGDAVHYDIVGPICESGDFLAHDRLLLLAEGDLLAIRSAGAYGMAMSSNYNARPRACE
                     VLVDGDVAHLIRRRETFDDLIAHERRLPA"
     gene            5967..6308
                     /locus_tag="VFO53_07145"
     CDS             5967..6308
                     /locus_tag="VFO53_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876087.1"
                     /translation="MMADMMSMMTPAASAEAPKKKARKRKAAAPKKAAGRKTTRKAAP
                     KKRAAKKATKKRAAPKKRAAKKTTKRKATKKRAAPKKRATKRGASKKKAAPRRKTGAR
                     KTAKKRATKRK"
     gene            complement(6451..7986)
                     /locus_tag="VFO53_07150"
     CDS             complement(6451..7986)
                     /locus_tag="VFO53_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET8876088.1"
                     /translation="MLYRRSLILPLVLALAACATTPAPEPPSPPLPAESTPLPVIDFA
                     PAPPSIVLADPFDGGVADLEPLPPSPGDLWDRIVRGYAMPDMDGPLVDKWEHWYAERP
                     DYVARMVERSRRYLYHIVGEVEARGMPSEIALLPIVESAFNPNALSVSRASGIWQFMP
                     QTGKTYGLTQNWWFDSRRDVIAATSSALDYLQRLNAEFNDWQLSLAAYNWGEGNVRRA
                     VARNRARGLPVDFASLQNVPVETRNYVPKLQAVKNIIADPQKYALALADVPNAPYFTV
                     VRTTVRLDVKRAAELAELPVDEFLALNPQHNRPVISGADEYAILLPIDKAEVFAAKLN
                     LVAQPLVSWQAHRMKRGETLTQVATRYGMSIETMRSVNGIGPRETVPTGYALLVPMQR
                     PSIEAAASLEDAVFTTVPANRTFYYTVRRGDTLTEIAGRYGVTIPDLKRWNHLARDSV
                     RTGQKLRVTSDVPPARSADRVRPVKASHAKARRHGSGAVHSASGGGGKRKASPMTTPA
                     AKP"
     gene            complement(8083..8859)
                     /gene="gloB"
                     /locus_tag="VFO53_07155"
     CDS             complement(8083..8859)
                     /gene="gloB"
                     /locus_tag="VFO53_07155"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HET8876089.1"
                     /translation="MISIIPIPAFRDNYIWALRRGALAAVVDPGDAAPVVAWLDANGV
                     ALTAILATHHHADHVGGIPALCARYGVPVFGPAHEAIPARTHPLGEGDRIGVPGIDLL
                     LSVFDIPGHTAGHIAYYTMPPDPLVLCGDTLFAAGCGRLFEGTPEQMWSSLSKLAALD
                     ESTRVYCGHEYTLANLRFAAAVEPASKDIEVRIVHERDKRERGLPTLPSTIGAERATN
                     PFLRAALPGVMACAAARAGRPVRDAVDAFATLREWKNAFA"
     gene            8858..9619
                     /locus_tag="VFO53_07160"
     CDS             8858..9619
                     /locus_tag="VFO53_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8876090.1"
                     /translation="MERSSDAVDSPSMTFATWLATPLGEYLKEREQAYFDLTVADIFG
                     FHALQIGLPECRLLAQSRIVSRWTLDYDPPADIIADPHGLPFAENTLDLIVMPHALEF
                     TDDPHLMLREAWRVIRPEGQIVIAGFNPFSLFGTKRYFGRGATPPWNGNFIALYRLKD
                     WLSLLGFDIIGGRFDCYVPPFAQDKWLRRFSFFERSGDRWWPITGGVYYLRATKKVLG
                     MRLLTPAWQRRERRKKALAPAAGARESLTTQRRRR"
     gene            9805..10248
                     /gene="rnhA"
                     /locus_tag="VFO53_07165"
     CDS             9805..10248
                     /gene="rnhA"
                     /locus_tag="VFO53_07165"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HET8876091.1"
                     /translation="MSGVIIYTDGACKGNPGPGGWGALLQFDGREREIFGGEPQTTNN
                     RMELTAVIRALEALTRTCDIELYLDSQYVKQGIESWIHTWKKNGWRTSDRKPVKNAEL
                     WRELDALRARHRIRWHWVRGHADTPGNHRVDELANRGVAQVNGSG"
     gene            complement(10240..>10522)
                     /locus_tag="VFO53_07170"
     CDS             complement(10240..>10522)
                     /locus_tag="VFO53_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET8876092.1"
                     /translation="KDSALLRYSIGNEHLKASEWDAAIDALEHAVALDATYTAAWKLY
                     ASALAHAGRHADALDAYRRGIVVARQKGDRQAEKEMTVFARRLERGSSA"
BASE COUNT         1687 a         3604 c         3570 g         1661 t
ORIGIN      
        1 aaggccgact acgtctatct gcgccgcacc gtacccggca tggcgctcga gcgcgcgctg
       61 caggaagcgc tcgacgtggt gctcgcgcgc cttccgattc ccaaggtcat gcgctacgcg
      121 gcagcgacca gctactacac cgacgtcgca ttcgtgcgtc cggcgcaccg gctcgtcgcg
      181 ctgcacggca gcgacgtcgt cccggtccgc gcgctcgggc tcgatgcggg gcgcatgagc
      241 tcgggtcacc gctttctcgc ccgcgcggat ctttcgatcg ccaccgccga cgcctatgcg
      301 ccgacgctcg aggcggaggg caaggtgctg ccgtcgttcg cgacgcgccg cgcggccatc
      361 gcgcgcgccc tcgaggcctg cgccggtgcc gcgcgggtca tcatgcccga tgcgctgctc
      421 gacgaggtga cggcgctcgt cgagtggccc gcggtgcatg aaggcgcgtt cgacgccgcg
      481 tttctcgacg tgccgcagga gtgcctgatc ctgacgatgc agcagaacca gaagtatttc
      541 gcgcttgccg acgcgcgcgg cgcgctcgtc ccgcgtttcc tcctcgtcag caacctcgac
      601 accgccgacg cctccgcgat cgtgcgcggc aacgagcgcg tgctgcgcgc ccggctcgcc
      661 gacgcgaagt tcttctacga ccaggaccgt aaggttgcgc tcgccgcgcg cgtcgagcgc
      721 ctcgcggcga tcgtctatca caacaagctc ggcacgctgc gcgatcgcgt cgagcgattg
      781 cgctttctcg cgcgtgacat cggcccgcac gtcggtggcg acctcgcgct gtgcgatcgc
      841 gccacgctcc tcgcgaaggc cgatctcgtc accgacatgg tgggggagtt tccggagctg
      901 caggggacga tgggacgcta ctacgcgcaa cacgacggcg agccggcgcc ggtcgcggac
      961 gcgatcgcgc agcactactg gccgcgctat gcgggcgatg cgttgccgca atcgccggtg
     1021 gcgcaggcgg tcgcgctcgc cgacaagctc gaagcgctcg cgggcctgtt cggcatcggc
     1081 gccgtgccga cgggagacaa ggacccgttc ggcctgcggc gcgccgcgat cggcgtgctg
     1141 cgcatcctga tcgaaaagcg catcgcagtc ccgctcgcgc atctggtcga tatcgccttc
     1201 gcggcgttcg acgcggtgcc cgccgtgaag ccggtgcccc gcgagctcgc cgattttctc
     1261 tacgagcggc tgcgcagcca cctgcgcgag caggggtata cggcgaatca ggtcgaagcg
     1321 gtgctcgcgc agcggccgca gcgcatcgac ctcgtgcccg accgcctcgc ggccgtgaag
     1381 gcgttcgaat cgctccccga atcgagcgca ctgtcggcgg ccaacaagcg catcgtcaac
     1441 atcctgcgca agagcggcac cgatgcggct ccgagcgtcg ccgtcgagcg gctgaccgat
     1501 ggcgccgagc gcgacctgtg gaacgtgttc gagcggctgt cgcccgacgt cgagcggcac
     1561 tgcgaaagcg gcgactatgc gagcgcgctg aagtcgctcg ccgccgcgaa gcccgccgtc
     1621 gaccgattct tcgacgacgt gatggtgatg gtcgacgatg ccggcgtgcg cgccaaccgg
     1681 ctcgcgctgc tcgaccgcgt cgcgcgcacg atgaaccgcg tggccgacat ctcgaggctg
     1741 gccgcctgat ccgcgtcgtc gcggattccc tctatccgat ggcgccgctc gcatccgaag
     1801 gcatcgtgca aagccgcgtc acgcggctca tcgttctcga ccgcgacggc gtcatcaatc
     1861 acgacagcga ccagttcatc aagtcgccgg acgaatggcg gccgattccc ggcagcctcg
     1921 aggcgattgc acggctaaat cacgccggct atcgcgtcgt cgtcgccacc aaccagtcgg
     1981 gcgtcgggcg tggattgctc gatttctcga cgctgtcggc catccacgac aagatgcacc
     2041 gcgcgctcgc gcacgtcggc ggacgcgtgg atgccatctt ctactgtccc catacggccg
     2101 attcgcgctg cgaatgccgc aagccgaagg ccggaatgct gcgcgagatc ggcgtgcgct
     2161 tcaatgtcga catgaccggt gtcgcgtgcg tcggcgacgg tttgcgtgac ctgcaggcgg
     2221 cgcagaccgt cggtgcgcaa ccgatgctcg tgctgaccgg caagggcgag aagacgctgc
     2281 gcgaaggcgg attccccggg aacaccgtga tctttcccga cctcgcattc gtcgcgtcgg
     2341 cgctgctcgt tgccgactga acgcatggag acggcgccgc gtgcaacctc ggcactgcgc
     2401 tcggttgcct tcgcgctgtt ccagatgctc gtcaccccgc cgtacgcgct cgccgtcgtc
     2461 gcgctcttct ggctgccgcc cgtgccgcgt tatcgcttca tcacgcattg gtgcgcgttg
     2521 aacctgtggg gtgcgcgtgt cctgtgcggt atccgccatc gcgtgatcgg aatcgagaat
     2581 gtcggcgatg cgccgaagat cgtcgcgtgc aagcacagct cgacgtggga gacgctgttc
     2641 ctgtcgcggc tgctgccgcc gctcgcctac gtcgcgaaga aggaactgct gtcggtgccg
     2701 ttcttcggct ggggcttccg gctggcgtcg ccgatcacga tcgatcgtga cgccgggcag
     2761 gatgcgatga cccagatcgc gacgcagggt cgcgagcgct tcgcgcaggg cttctggatc
     2821 atcctgtatc ccgagggaac gcgcatcccc gccggcaagc gtgtgcgcta caaaaccggc
     2881 gcggcgcggc tcgcaatcga gatgcgcacg ccgatccttc ccatcgcgca caacgccggt
     2941 tggctgtggc cgaaaggggt gatgggcaag cgtccgggca cgatcacgct gtcgatcggc
     3001 aagccgatcg acccgggaac gatggatccc gcgcggctga tgcagaccgt cgagcggtgg
     3061 atcgaggccg aagttgcgcg cctcggcgaa gcatgaagct cgagccgctc gtcgccgacg
     3121 atccgcgcat tgcaaacggc acgctgcgcc gcgtggtgct cgccggccag gccgtcgatt
     3181 accggctgat ccgcgcgcgc cggcgctcga tcggcatgga agtgcatttc gaagggctga
     3241 cggtgcgcgc gccgcgctgg gtgacgctgc gcgacatcga agccgccctc gtcgaacgcg
     3301 ccgcatggat cctcagggca ctcgccgaat ggcgtgcacg acggcgtgac gtcatgcccc
     3361 gcacctgggt gtcgggcgca accatcgcgt ttcgcggtgc agcactgacg ctcgcgctgt
     3421 atccggcgcg ccagcccgcc gtcgatgcgg atctcttcca tgtaaccgtg cgccacccga
     3481 atgcgcacga cgagcaccag gtcgccgact gcgtgcgcgc ctggctgcgc gatcaggcca
     3541 ccacgctcgt tgccgcgcgt gtcgcccact acgtgcagcg ggtcggcacg cacatgccgt
     3601 cggtccggct gtcgaacgca cgcagcgagt ggggaagctg caacgcgaag ggcgagattc
     3661 gcctcaactg gcggctcgtg caattgcccc cgccgctcgc cgaatacgtg gtcgcgcacg
     3721 aggtcgcgca catcgtcgag ctcaaccatt cgcgccggtt ctgggcgctc gtcgaagcgc
     3781 tgctgcccgg ccattcggca ttgcgcgagc agctcgacga ctggacggcg ctgctcgcgg
     3841 cgtgacgcgc ggcgtcagct cgccggcagt tcgggcatgc gcaccgcgat ccgcgcctgc
     3901 gccttgagga ggcgcaccag cgctgccgcg agctcgtcgc tgccgcggac atcgcgccag
     3961 cggccgtcct gcgcacgaaa gtgatagccg ccgcttttcg ccgccaccca gatctcgcgg
     4021 ttcggcaggt gccggttgac gatgatgcgg ctttcgtcgt cgcattcgat cgtcagcacg
     4081 ccatcgttca acgaccagtc gagctcggcg tcgctcgttt ccagggccgc gtcgagcgcg
     4141 aggccgatgc gctcgagcgt cgcgtcggcg gcggcgagga aggcggattc gtccatcacg
     4201 ccatgctagg attcacggcg atgccacgtc aatccattca tgcgcgtgcg ctcgttgccg
     4261 cgacgctcgc agtgctcgtc gcgatcgtgt ccgcctgcgg catcaagggg ccgctgcgtc
     4321 ccgcgccgaa gacgacgccc gcgacggcga caccgccggc caccgcgaat ccggccacac
     4381 cgccggccac gacatcgccc gcgctgccac cgccgagctc gccgccaccg aagtgaccgc
     4441 cgcccacggc gcagccgggt tcgattacgc gggcggcgag ctctgcgccg agcgcgtgcc
     4501 gctgtcggcg ctcgcgcagc gctacggtac gccgtgctac gtctattcac gcgcgctgct
     4561 ggcgcgcgcc ttccacgaat tcgacgcggc gttcggcggc gtgccgcatc tcgtctgcta
     4621 cgcgatgaag gcgaacccgt cgctcgcgat cgtcgacctc ttcgcctcgc tcggcagcgg
     4681 cttcgacatc gtttccggcg gcgagcttgc gcgggtgcag gcggcgggcg gcgacgccgg
     4741 caaggtcgtg ttctccggcg tcgccaagag cgacgcggaa atggaggcgg cgctcgctgc
     4801 gggcatcctg tgcttcaacg tggaatcggc ggccgagctc gaccatctcg ccgcagttgc
     4861 cgcacggagg ggcgttcgcg cgccggtcag ctttcgcgtg aatcccgacg tcggtccgcg
     4921 cacgcaccgc tatatcgcca ccggcttgaa ggagagcaag ttcggtgtcg cgttcgacga
     4981 ggcgcacgcg ctgtaccggc gtgctgccgc catggcgccg atccgcgttc gcggggtcga
     5041 catgcacatc ggctcgcaga tcaccgagct tgcgccgtac cgggaagcgc ttgcgagagt
     5101 gctcgggctg gtcgacgcgc tcgccgccga cgggatcgca ctcgagcaca tcgacgtggg
     5161 cggtggactc ggcgttcgct atcgggacga aacgccggtg gacgtcggcg attacgcggc
     5221 gatggtcggc gagctcatgc gcgggcgccg cgagctgctg ctgctcgagc cggggcggcg
     5281 gctcgtcgcc gacgcgggcg tgctgctcac ccgcgtgcgc gttctcaagg ctggcggcgc
     5341 gcgcaacttc gcgatcgtcg acgctgcgat gaacgacctg ctgcgtccct cgctctatgg
     5401 cgcgtggcac ggcgtcgacg ccgtgcgccc gcgccagggc gacgcggtgc actacgacat
     5461 cgtcgggccg atctgcgaaa gcggcgactt tctcgcgcat gaccggttgc tgctgctcgc
     5521 cgagggcgac cttctcgcca tacgaagtgc cggtgcatac ggcatggcga tgagctcgaa
     5581 ctacaacgca cgcccgcgag cgtgcgaagt gctcgtcgac ggcgacgtcg cgcacctcat
     5641 ccgccgccgc gaaacgttcg acgacctgat cgcgcacgag cgtcgactgc cggcgtgaaa
     5701 acgcgcgcgg tgttccgcgt gagagcgcaa tttcgcgtgc aatcggcgtg tgcgaattcg
     5761 caacaacgcg cgatcgggcg tcgccgcacc tgttcgaaat cttgcaaatg cgcgaaacgt
     5821 ggctacaatg caggcagcag gtgccaatgg atttgtcaag ggggtaggct cgaatttgat
     5881 ccatcaagcg cttttccccg tgcgaccggc gacacctgcc acgaaggacc ataacagtca
     5941 catgaactca gacaggagaa gcaaagatga tggctgacat gatgtcgatg atgacgcctg
     6001 cggcttcggc cgaagcgccg aagaagaagg ccaggaaacg caaggccgcc gcgcccaaga
     6061 aggcggcggg tcgcaagacc acccgcaagg ccgccccgaa gaagcgggct gccaagaagg
     6121 cgacgaagaa acgcgccgcc ccgaagaagc gggctgccaa gaagacgacg aagcgcaagg
     6181 cgacgaaaaa gcgggccgcc ccgaaaaagc gggcgaccaa gcgaggggcc agcaagaaga
     6241 aggccgctcc gcgccgcaag accggcgcgc ggaagaccgc caagaagaga gccacgaaga
     6301 ggaagtaacg cttcttcgat ccgattccgt cgcagtcgtg tgacggacat gaaacgggcg
     6361 gcgcctaggc gtcgcccgtt tcgcatcggg gccggagacg attgatttgc gggcccgcca
     6421 gatagatcgt cgctgaccct gcggcggttt ctacggtttc gccgcgggcg tggtcatcgg
     6481 cgacgccttc cgcttgccgc cgccgcccga tgcgctgtgc acggcgccgc tgccgtggcg
     6541 gcgcgccttc gcgtggctcg ccttcaccgg gcgcacgcgg tccgcgcttc gcgcgggagg
     6601 aacatcgctc gtcacgcgca gcttctggcc ggtgcgcacc gagtcgcgcg cgaggtggtt
     6661 ccagcgcttc aaatcgggga tcgtcacgcc atagcggccg gcgatctcgg tcagcgtatc
     6721 gccgcgacgc accgtgtaat agaacgtccg gttcgcgggc accgtggtga acacggcgtc
     6781 ctcgagcgac gcggccgcct cgatggacgg acgctgcatc ggcaccagca acgcataccc
     6841 ggtgggcacg gtttcgcgcg gaccgatgcc gttgacggaa cgcatcgttt cgatcgacat
     6901 gccgtagcgc gttgccacct gcgtcagcgt ttcgccccgc ttcatccggt gcgcctgcca
     6961 tgagacgagc ggctgcgcca cgaggttgag cttcgcggcg aagacttccg ccttgtcgat
     7021 cggcagcaga atcgcgtact cgtccgcgcc ggaaatcacc ggccggttgt gctggggatt
     7081 gagcgccagg aattcgtcga ccggcaattc ggcgagctcg gccgcgcgct tcacgtccag
     7141 gcgcacggtg gtgcgcacga cggtgaagta cggcgcgttc gggacgtcgg ccagtgccag
     7201 cgcgtacttc tgcggatcgg cgatgatgtt cttcaccgcc tgcagcttcg gcacgtagtt
     7261 gcgcgtttcg acgggcacgt tctgcaggct cgcgaagtcc accggcagtc cccttgcacg
     7321 gttgcgcgcc accgcacggc gcacgttacc ctcgccccag ttgtacgcgg cgagcgacag
     7381 ttgccagtcg ttgaactcgg cgttcagtcg ctgcaggtag tcgagcgcgg aactggttgc
     7441 ggcaatcacg tcacgccgcg aatcgaacca ccagttctgc gtcaatccgt aggtcttgcc
     7501 cgtttgcggc atgaactgcc agatgcccga cgcacggctc accgagagag cgttgggatt
     7561 gaatgcgctc tcgacgatgg gcagaagcgc gatctccgac ggcatgccgc gcgcctcgac
     7621 ctcgccgacg atgtgataca ggtaacggcg gctgcgctcg accatgcgcg ccacgtagtc
     7681 ggggcgctcc gcataccagt gctcccattt gtcgacgagc gggccgtcca tgtcgggcat
     7741 ggcatagccg cgcacgatgc gatcccacag atcgcccggc gacggcggca gcggctcgag
     7801 atccgctacg ccgccgtcga acggatcggc gagaacgatg ctcggcggcg caggtgcaaa
     7861 gtcgatcacc ggcaatggcg tcgactcggc gggcaacgga gggctcggcg gctcgggtgc
     7921 gggcgtcgtc gcgcacgcgg cgagcgcgag caccaacggc aggatcaggc ttcgtcgata
     7981 gagcactaag gaatcgcggt ttctaatgca cgccgtaacg cacaaaaatt ccggcgatgg
     8041 taggccgcat ccaaccgagc gtcaaccgcc acatcgcgcc ggtcacgcaa atgcgttctt
     8101 ccattcgcga agcgtcgcga acgcgtcgac cgcgtcgcgc acggggcgcc cggcgcgtgc
     8161 cgcggcgcac gccatgacgc cgggcagcgc ggcgcgcagg aacggattgg tcgcgcgctc
     8221 ggcgccgatc gtcgacggaa gtgtcggcaa accgcgctcg cgcttgtcgc gctcgtgcac
     8281 gatgcggacc tcgatgtcct tgctcgcggg ctcgacggcc gccgcgaagc gcaggttcgc
     8341 gagcgtgtat tcgtgcccgc aatagacgcg cgtgctctcg tcgagtgctg cgagcttcga
     8401 cagcgacgac cacatttgtt cgggcgtgcc ttcgaacagc cgcccgcagc cggccgcgaa
     8461 cagcgtgtcg ccgcagagca cgagcggatc gggcggcatc gtgtaatagg caatgtgccc
     8521 ggcggtgtgg cctggaatgt cgaacacgga cagcagcaga tcgatgccgg gcacgccgat
     8581 gcggtcgcct tcgccgagcg ggtgggtgcg cgcgggtatc gcctcgtgcg cgggaccgaa
     8641 cacgggcacg ccgtagcgcg cgcaaagcgc cggtatgccg ccgacgtgat cggcgtgatg
     8701 atgcgtggcg agaatggcgg tcaacgcgac gccgttcgcg tcgagccatg caacgacggg
     8761 cgcagcatcg ccgggatcga cgacggctgc cagagcgccg cgccgcagcg cccagatgta
     8821 gttgtccctg aatgcgggga tggggataat cgagatcatg gagcgctcct cggatgccgt
     8881 ggacagcccg tcgatgacgt tcgccacctg gctcgcaaca ccgctcggag agtatctcaa
     8941 ggaacgggaa caggcgtatt tcgacctgac cgtcgcggac atcttcggct tccatgcgct
     9001 gcagatcggc ctgcccgaat gccgcctgct cgcgcaaagc cgcatcgtct cgcgctggac
     9061 gctcgactac gacccgcccg ccgacatcat cgccgatccg catggcctgc cgttcgccga
     9121 gaacacgctc gacctcatcg tgatgccgca cgcgctcgag ttcaccgacg atccgcacct
     9181 gatgctgcgc gaagcgtggc gcgtgatacg ccccgagggc cagatcgtca tcgcgggctt
     9241 caacccgttc tcgctgttcg gcacgaagcg ctacttcggc cgcggcgcga cgcccccctg
     9301 gaacggcaac ttcatcgcgc tctatcggct gaaggactgg ctgtcgctcc tcggcttcga
     9361 catcatcggc ggacgcttcg attgctacgt cccgccgttt gcgcaggaca agtggctgcg
     9421 gcgcttttcg ttcttcgagc gcagcggcga ccgctggtgg ccgatcaccg gcggcgtgta
     9481 ctacctgcgc gcaacgaaga aggtgctcgg catgcgcctg ctgaccccgg catggcagcg
     9541 tcgagagcga cgcaagaagg cgctcgcgcc ggccgcgggc gctcgcgaga gcctcaccac
     9601 gcagcggcgg cgccgatgag cgcgccgggc cgccccaagc gcgaatcgag cccgcagcgc
     9661 gaagtgctgc gggaaatcca ttgagcgcgt cgggccgccc caagcgcgaa tcgagcccgc
     9721 agcgcgaagt gctgcgggaa atccattgag cgcgccgggc cgccccaagc gcgaatcgac
     9781 cgcgcagcgc aaagcgccga tccaatgagc ggtgtgatca tctacaccga cggcgcatgc
     9841 aagggcaatc ccggccccgg cggctggggc gcgcttctgc agttcgacgg gcgcgagcgg
     9901 gagatcttcg gcggcgagcc gcagacgacg aacaatcgga tggaactgac ggcggtcatc
     9961 cgtgcgctcg aggccctcac gcgcacctgt gacatcgagc tctatctcga ttcgcaatac
    10021 gtgaagcagg ggatcgaaag ctggatccac acgtggaaga agaacggctg gcgcacgagc
    10081 gaccgaaagc ccgtgaagaa cgccgagctg tggcgcgagc tcgacgcatt gcgtgcccgg
    10141 caccgcatcc gctggcactg ggtgcggggt cacgcggaca cgcctggaaa tcaccgcgtc
    10201 gacgagctcg cgaaccgcgg cgtggcgcag gtaaacgggt caggctgacg acccgcgttc
    10261 gagccgccgc gcgaacaccg tcatctcctt ctccgcctgc cggtctccct tctgtcgtgc
    10321 gacgacgatg ccccgccggt acgcgtcgag cgcatcggca tggcgccccg cgtgcgcaag
    10381 tgcgctcgca tacagcttcc acgctgcggt gtacgtcgcg tcgagcgcaa cggcgtgctc
    10441 gagcgcgtcg atcgcggcgt cccactcgct cgccttcagg tgctcgttgc cgatcgaata
    10501 gcgcagcagc gcactgtcct tg
//
LOCUS       DASSVX010000169         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_406700, whole genome shotgun sequence.
ACCESSION   DASSVX010000169 DASSVX010000000
VERSION     DASSVX010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406700"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1787
                     /locus_tag="VFO53_07175"
     CDS             <1..1787
                     /locus_tag="VFO53_07175"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET8876093.1"
                     /translation="NPQAGSDAAAIPDHGIVCYGEHDGARVLGLRVTWDKRYITLGPV
                     ATLLGLAFRAYDPDRLAGEREDLGITCALIPATHPGVEIGRRHMPLNAVFQNGPNSGR
                     DVFIPIDWVIGGAPMLGKGWRMLMESLAAGRGISLPSSNTGMGKLAVRTTGAYARVRT
                     QFKTPIGRFEGIEEALTRMGGNLYVMDAARMLTATLVDRGEKPSVLSGIAKLHITERG
                     REIINDAMDIAGGKGICMGPSNFLGAAYMQMPVSITVEGANILTRSLIVFGQGAVRCH
                     PFVVREMAATQERNRRKALRDFDAALFGHMRFAVRNFLRTLVLGITGSHFVRVPRDVA
                     PDARRYYQQATRISSSLALIADVTMATLGGALKRKEKISARLGDVLSQLYLCSATLWR
                     YEQDGRPAADAPLMHWAIRDAMFKAQTAIDGVLENFPSPMLGALMRRVVFPLGRPYVV
                     PSDALGHDVARCLIEPGATRDRLTAGMFIGAADEPVGLLERALAVTVMAEPVERRLAD
                     AMRSGTLAARGTPGEGASELAARAVSAGVISQAEAVLLVSQRELAAAVVRVDDFDQDL
                     GTSLLRTAPGGGAGVHRSQSTAAQRAVA"
     gene            1791..>2089
                     /locus_tag="VFO53_07180"
     CDS             1791..>2089
                     /locus_tag="VFO53_07180"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743948.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET8876094.1"
                     /translation="MEATIGNSDEAAGRAGRSPIYIVDGARTPFLKSRNRPGPFAASD
                     LAADAGRALLLRQPFLPSELDEVILGCAAPSVDEVNIGRVAALRMGCGNKVPGWTV"
BASE COUNT          307 a          713 c          732 g          337 t
ORIGIN      
        1 cgaacccgca ggcgggctcc gacgccgcgg ccattcccga ccatggcatc gtctgctacg
       61 gcgagcacga cggggcgcgc gtgctcggtt tgcgcgtcac ctgggacaag cgctacatca
      121 cgctcggccc cgtcgcgaca cttctcggcc tcgcgttccg cgcctacgat cccgatcgcc
      181 tcgccggcga gcgcgaggat ctcggcatca cgtgcgcgct cattcccgcg acacacccgg
      241 gggtcgagat cgggcggcgg cacatgccgc tcaacgccgt gttccagaac gggcccaatt
      301 ccggccgcga cgtcttcatt ccgatcgact gggtgatcgg cggtgcgccg atgctcggca
      361 aggggtggcg gatgctgatg gaatcgctcg cggcgggacg cggcatatcg ctgccctcgt
      421 cgaacacggg catgggcaag ctcgccgtgc gcaccacggg cgcctatgcc cgcgtgcgca
      481 cgcagttcaa gacgccgatc ggccgcttcg agggcatcga ggaggcgctc acgcgcatgg
      541 gcggcaacct ctacgtgatg gatgccgcgc gcatgctcac cgcgacgctc gtcgatcgcg
      601 gcgaaaagcc ctccgtgctg tccggcattg cgaagctgca tatcaccgaa cgcgggcgcg
      661 agatcatcaa cgacgcaatg gatatcgccg gcggcaaggg catctgcatg gggccgtcga
      721 atttcctcgg cgccgcgtac atgcagatgc cggtgtcgat cacggtcgag ggcgcgaaca
      781 tcctgacgcg cagcctcatc gtgttcgggc agggtgcggt ccgctgccac ccgttcgtgg
      841 tgcgtgaaat ggcggcgacg caggagcgca accggcgcaa ggcattgcgc gatttcgacg
      901 ccgcactctt cggccacatg cgcttcgccg tgcgcaactt cctgcgcacg ctggtgctcg
      961 ggatcaccgg ctcgcatttc gttcgcgtgc cgcgcgatgt cgcgcccgat gcccggcgct
     1021 actaccagca ggcgacgcgc atttcgtcgt cgctcgcgct gatcgccgat gtgacgatgg
     1081 cgacgctcgg cggcgcattg aagcgcaagg agaagatttc ggcgcggctc ggcgacgtgc
     1141 tgtcgcagct ctacctctgc tcggccacgc tgtggcgcta cgagcaggac gggcgcccgg
     1201 cggccgacgc gccgctcatg cactgggcga tccgcgatgc gatgttcaag gcgcagaccg
     1261 cgatcgacgg cgtgctggaa aatttcccga gcccaatgct cggcgcgtta atgcgccgcg
     1321 tcgtgtttcc gctcggccgt ccgtatgtcg taccctccga tgcgctggga cacgacgtgg
     1381 cgagatgcct gatcgagccg ggcgcaacgc gcgaccggct gaccgccggc atgttcattg
     1441 gcgccgccga cgagccggtc ggcttgctcg agcgcgcact cgcggtgact gtcatggccg
     1501 agccggtcga gcgcaggctg gccgacgcca tgcgcagcgg aacactcgca gcacgcggca
     1561 cgccgggcga aggtgcaagc gagctcgccg cgcgtgcggt ctcggcaggc gtgatttcgc
     1621 aggccgaggc ggtcctgctc gtgtcgcagc gcgagctggc cgcggctgtc gtgcgtgtcg
     1681 acgatttcga ccaggacctc ggcacgtcgc tgctgcgcac tgcgccgggc ggcggcgcgg
     1741 gcgtgcatcg atcgcaatcg acggctgcgc agcgcgcggt tgcgtgacgg atggaagcga
     1801 cgatcggcaa cagcgacgaa gcggcgggcc gtgccgggcg ctctccgatc tatatcgtcg
     1861 acggtgcgcg cacgccgttt ctcaagtcgc gcaaccgccc gggaccgttt gcggcgtcgg
     1921 atctcgctgc cgacgccggc cgcgcgctgc tgctgcgcca gccgttcctg ccgagtgagc
     1981 tcgacgaggt gatcctcggc tgcgcggcac cctccgtcga cgaggtgaac atcggacgcg
     2041 ttgccgcgct gcgcatgggt tgcggcaaca aggtgcccgg atggacggt
//
LOCUS       DASSVX010000170        25928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_590133, whole genome shotgun sequence.
ACCESSION   DASSVX010000170 DASSVX010000000
VERSION     DASSVX010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 25928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25928
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590133"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VFO53_07185"
     CDS             <1..929
                     /locus_tag="VFO53_07185"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET8876095.1"
                     /translation="TVCLFGGAPYPPQYRELERGVDVLVATPGRLIDHIERGRVDLSG
                     LSMLVLDEADRMLDMGFQEDVERICSRAPAARQTVLFSATFDGAAGRLAAELSRDAVR
                     IDVRTTRTAPVAIEQRVHFADDRAHKYRILDHLLADVGTGQSIVFIATRRDAEMLAGK
                     LQDEGHTAAALHGDMNQRERDRTLTRMRRGGVRTLVATDVAARGLDVPGVTHVINFDL
                     PRQAENYVHRIGRTGRAGASGVAVSLANATERGTLRQIERYTGAAIPAHVIPGLEPRV
                     RTQNGVPKRPYGGGMMKGRGADRRNPRGRPGR"
     gene            948..1979
                     /gene="paaA"
                     /locus_tag="VFO53_07190"
     CDS             948..1979
                     /gene="paaA"
                     /locus_tag="VFO53_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HET8876096.1"
                     /translation="MYAQMVETGLKKVQDAADMTPEDVAFQARIDADVKIEPKDAMPE
                     AYRRTLIRQIQQHAHSEIVGMLPEGNWITRAPSLQRKAILMAKVQDEAGHGLYLYCAA
                     ETLGVSRDRMIDDLHAGKVKYSSIFNYPTLTWADIGTIGWLVDGAAIMNQIPLCRCSY
                     GPYARAMIRVCKEESFHQRQGFDIMMALCRGTPEQKAMAQDALDRWWWPSLMMFGPPD
                     AASVHSAQSARWKIKLLSNDELRQRFVDQTVPQADYLGLRIPDDALVFNEERGHYDFG
                     AIDWNEFHEVLKGHGPCNRDRMRARVSAWEDGAWVREAALAHADKRAARERAATQPAP
                     GLQRQMEAG"
     gene            1976..2260
                     /gene="paaB"
                     /locus_tag="VFO53_07195"
     CDS             1976..2260
                     /gene="paaB"
                     /locus_tag="VFO53_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HET8876097.1"
                     /translation="MSSEWPLWEVFIRSQHGLAHKHVGSLHAVDAEMAIRNARDVYTR
                     RNEGVSIWVARSSDIHASSPGDRAALFEPSNDKVYRHPTFFPMPDGVKHL"
     gene            2253..3011
                     /gene="paaC"
                     /locus_tag="VFO53_07200"
     CDS             2253..3011
                     /gene="paaC"
                     /locus_tag="VFO53_07200"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HET8876098.1"
                     /translation="MFSPLCDYALRLADNDLVLAQRLGEWVGKGPVLEEDIASTNVGL
                     DLLGQARLWLAYASEIDDGSEDRFAYFRDSTEFRNLLLVEQPNGNYADTLVRQYLFDQ
                     WHVLLLAQLSTSRDARIASIATKALTEVHYHVERSAGWMIRLGDGSDLSHARMQAALD
                     ALWMYTGEMFAVDATERALIDAGIAADVAALHAPWLAALQPVLAEATLTMPDDAWMQG
                     SSGRGGKQGVHTEHLSRMLAEMQVLPRTYPDAHW"
     gene            3068..3538
                     /gene="paaD"
                     /locus_tag="VFO53_07205"
     CDS             3068..3538
                     /gene="paaD"
                     /locus_tag="VFO53_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HET8876099.1"
                     /translation="MWRALGTVADPELPAVSIVELGIVREVACTADECIVTLTPTYSG
                     CPATRMIEADAKAAVASVTDARVRLVTALTPAWCSDWIAPEARRKLAASGIAPPRHGG
                     AQPAIPIAVDRGAIRCPNCGSRRTEEIARFGSTACKAQHRCRDCLEPFDYFKQH"
     gene            3557..4636
                     /gene="paaE"
                     /locus_tag="VFO53_07210"
     CDS             3557..4636
                     /gene="paaE"
                     /locus_tag="VFO53_07210"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HET8876100.1"
                     /translation="MSRFHRLPIARIDRETRDAITITFDVPASLREQFRFDAGQHLTL
                     RAEIDGEPVRRSYSICAAEGDTLRIAVKRAPGGRFSAWANESLRAGTHIDVTQPMGRF
                     NVVRDPRESRHYLGVAAGSGITPLLSMLATSLEAEPRSRFTLIYANRSSASVMFRDEL
                     AALKDRHLSRFNLVHVLSREPQDIELLHGRVDRAKADALFERWVPIGDVDYAFVCGPE
                     GMMDAVRASLVAHGLAEQRIRIERFAASIPKHTHVARPLPAAARTECEVTVVIDGSQR
                     TFLLEKGRENIIDAGLRNGIELPFSCKGGVCSTCRCRLVAGEVDMDVNFALEDYEVAR
                     GFVLACQSYPVTDRITVDFDQSGTG"
     gene            4734..6419
                     /gene="paaN"
                     /locus_tag="VFO53_07215"
     CDS             4734..6419
                     /gene="paaN"
                     /locus_tag="VFO53_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HET8876101.1"
                     /translation="MTHPFFERHRATLERAVEAIATRGFWTPFPESPSPKVYGEGTAE
                     AGRAAFDKLANQRFALTQPATIGQVGGERSPYGIRLGITYPKPDVDGLMSAVEAAEAS
                     WRKAGPQAWVGVSLEILDRINKASFEIAHAVMHTTGQAFVMAFQAGGPHAQDRGLEAV
                     AYAWDQMRRVPAQAAWEKPQGKGEPVRMEKRFRIVPRGVALVIGCSTFPTWNGYPALF
                     ASLATGNAVIVKPHPNAILPLAITVRIARDVLRDAGFDPNVVTLVAHEAGDDIAQSLA
                     LRPEIKIVDFTGSTANGRWLEEHARQAQVYTEKAGVNQVIVDSAADFKGLVRNLAFSL
                     SLYAGQMCTAPQNIYVPKAGIDTAEGHLSFDDVAAALAQGVERLLADDTRAVEVLGAV
                     ANDGVLRRLEAARSLGDIALDTRTITHAQFPDAVIRTPLVVKLTRADRATYLNEWFGP
                     IAFVVATNSTDDSLAIARDAVVGHGALTMSVYSTSDDVVARAIEVAEDVGVALSINLT
                     GGIFVNQSAAFSDFHGTGANPAANAALTDAAYVASRFRVVQHRMHAPQAQEAS"
     gene            6416..7207
                     /gene="paaG"
                     /locus_tag="VFO53_07220"
     CDS             6416..7207
                     /gene="paaG"
                     /locus_tag="VFO53_07220"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HET8876102.1"
                     /translation="MTYEQIRFECGDGIARITLDRPDRLNSFTTQMHEELRDALRRVA
                     TDPSARVLLLTGAGRGFCAGQDLADRAVAPGDAPVDLGASIENHYRPLVCALRDLPLP
                     VVCAVNGVAAGAGANVALACDLVIATRSASFIQAFCRIGLIPDAGGTFFLPRLVGSAR
                     AMGLALLGDKLTAEQAEQWGLIWKCVDDDAFTSSVDALLAQLAQAPTGGLAAIKRTLH
                     ASAGNTLDAQLTLERDTQRVLGQSADYREGVTAFLAKRPAHFTGR"
     gene            7210..8754
                     /locus_tag="VFO53_07225"
     CDS             7210..8754
                     /locus_tag="VFO53_07225"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011746546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HET8876103.1"
                     /translation="MTGAPIARDATVAVVGAGTMGCGIAQVAAQAGHRVRLFDTRLGV
                     AEKARASIGQTLNALAAKGKLDAGAVEAAAARIAVVHALGDCVSARLVIEAIVEDLAE
                     KRSLLRELEKVVTASAILASNTSALSITALASGLRFPGRVVGMHFFNPVPLMQLVEIV
                     SGLATDDAVADTVAATAAAWGKQPVRATSTPGFIVNRCARPFYGEALRLLAERAADVP
                     TLDALMRDAGGFRMGAFELMDLIGNDVNFAVTRGVWAACYHDPRYAPSMLQEERIAAG
                     YLGRKSGRGFYDYAEGSSRPAPASVSAQPAPKRLVVRGELGAVSPLVRRFDLAGIAVE
                     QAPARDAFADGAIEIGDAVLALSDGRTATARAAATGAKNLVLIDLALDYARCNRIGLA
                     RADTCPIAAFETAAGALQAAGVAASPLDDVAGLVVLRTVAALANEAADAVTHGVASAH
                     DVDAAMQKGVNYPRGPLRWADEIGLRRVRNVLVNLAAHYGEDRYRLSPLIARRYAAGA
                     PLADGG"
     gene            8765..9220
                     /gene="paaI"
                     /locus_tag="VFO53_07230"
     CDS             8765..9220
                     /gene="paaI"
                     /locus_tag="VFO53_07230"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HET8876104.1"
                     /translation="MNDDANPGREIAQSLAERVAARMFDADRASRALGMHIECVAPGR
                     AEMTMTVRDDMLNGHAICHGGYIFLLADSAFAFACNSYNRNTVAQGCTIDYLAPARAG
                     DVLRATGVERSRTGRTGVYDIDVTNQQGTTIALFRGKSYRVDGNIVDER"
     gene            9243..10544
                     /gene="paaK"
                     /locus_tag="VFO53_07235"
     CDS             9243..10544
                     /gene="paaK"
                     /locus_tag="VFO53_07235"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HET8876105.1"
                     /translation="MPATSPREADLEPIETASRDELAALQVQRLRWSLRHAYDNVAHY
                     RAAFERAGVHPGDLETLDDLARFPFTTKADLRDNYPFGMFAVPRTDVVRVHASSGTTG
                     NPTVVGYTRNDIETWAAVMARSIRAAGGRAGDIVHIAYGYGLFTGGLGAHYGAERLGC
                     TVVPMSGGQTAKQVQLINDFRPDIIMVTPSYMLAIAEEMQRQGLDPRESSLSIGIFGA
                     EPWTPTMRTAIETMLDIDAIDIYGLSEVIGPGVAQECIETKDGLTVWEDHFYPEIVDP
                     VTGAVLPEGEKGELVFTSLTKEAMPVVRYRTRDLTRLLPGTARTMRRIEKITGRCDDM
                     IIIRGVNVFPTQIEELILRDPMLAPHYQVEIARPRNLDEMTVLVERAPAAGGTDAEAA
                     GERLARRIKDLVGVSCAVRVTQSGGIERSLGKAKRVVDKRR"
     gene            complement(10567..12657)
                     /locus_tag="VFO53_07240"
     CDS             complement(10567..12657)
                     /locus_tag="VFO53_07240"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HET8876106.1"
                     /translation="MYRSPVVAYCLALLVALFAAPALAATFTVSNTNDSGAGSFRQAL
                     QDANGTVGGDTIVFSIPGAGVHTITLTSLLPLITSPVVIDGYTQPGSVANTNPMNAGI
                     NAVLQIELTGTQVSLFFAPGSDGSKVRGLVINGSSNDKIDGQSSNLTVTGNFLGTNAT
                     GTATASGSFGGFGVRIEGSAANATIGGPNPADRNLIAGNAQGGIILPFPSTSGHLIQG
                     NYIGTDVTGTVALGSGEGLDLMANASVLGNLVSGNPGGGINLLDNAVLRGNLIGTARD
                     GTTPLPNGNFGGISVDGNGSTIGGSGAGEGNVIAFNVNSGIDSFINLFGNRITQNSIH
                     SNTALGITLLHSGTPLANDAGDADVVPGNHGQNYPVIASVAIAAGTATIAGTINSNAG
                     APLHLEFFANATCDASGNGEGQSFIGAIDVTTDGSGNASFGPLAFAVPLGQGVITSTA
                     TSAGGDTSEFSACAAAVSGASSTSVASSLNPSTVGQSVTFTATVTGNSPTGSVQFKNG
                     VVNLGGPVALNGSGVAALATSALTQGSHAITAVYGGDANNATSTSAVLTQVVNAMPAG
                     ATTTALASSLNPSTLGQAVTFTATVSGASPTGSVQFFNGASSLGTIALGGGGTAALTT
                     SALTQGSHTITAVYSGDASNAASTSPAVQQVVNAGGVPPPPGTVQPIPTMSELALLLL
                     GALVAVSGIAGRRR"
     gene            12928..13188
                     /locus_tag="VFO53_07245"
     CDS             12928..13188
                     /locus_tag="VFO53_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876107.1"
                     /translation="MNDEKIPVRVTSSGQTLEVVVFDKRLDVIQVVLGEGVHSVRCDL
                     TPTRNGLAYVGNARGREIVYERTREQVKADLARAAGFRDFKR"
     gene            complement(13192..14268)
                     /locus_tag="VFO53_07250"
     CDS             complement(13192..14268)
                     /locus_tag="VFO53_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET8876108.1"
                     /translation="MIVAHEPIQRPPDAKLLAIDAAGRIAHWPRRAFPALLSRGDLVI
                     ANDAATLPASLCGVHVASGENIEVRLARRGSLDPEDVREFAAVIFGAGDHRTPTEDRR
                     PPPELAPGDVLTLGPLRATVRGLLGHPRFAALRFDGTAQAIWRGLASHGRAIQYAYMR
                     PALELWDVWTAFAGTPAAFEAPSAGFALDWALLAEMRRRGIEFATLTHAAGISSTGDE
                     ALDALLPLDEPYRIPRSTADAVARTRERKRRVIAVGTTVVRALEAAATVIGLVRPGAG
                     VATTRIVPGTRLRVVDAVLSGTHEPGTSHYALLGAFVSAATLRDADRELEQHGYRTHE
                     FGDSVLVERAAPSVVPGSAQGRAP"
     gene            complement(14348..15085)
                     /locus_tag="VFO53_07255"
     CDS             complement(14348..15085)
                     /locus_tag="VFO53_07255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8876109.1"
                     /translation="MNRTDDHRNDLAGLRIAITGGTSGLGLALVREWSARGAGVAFVA
                     RTPGSVERIAREIPGAAGIVGDVSTKAGIHPIALQILGRLGGLDVLVNNASSLGPVPL
                     ALLADTECEDFETALATNLLGPFRLTRTLLGALRASAAEGRGAVVLNISSDAALTPYS
                     HWGAYGASKAALRHMSAIWRNELQDEGIGVLALDPGDMDTPLHALAIPDADPSTLKAP
                     QSSARELTCAIVAELRELAQRKRSREE"
     gene            15177..15746
                     /locus_tag="VFO53_07260"
     CDS             15177..15746
                     /locus_tag="VFO53_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8876110.1"
                     /translation="MDKPPLVSAHLARNLAALRQVRSLTQEALAKSAGVPRSTIANLE
                     SGEGNPSLAVLVKVAEALSTSIDELVASPRAKVRRWAANDVASRRRGRGVTTRALVPE
                     PVPEEVMELMQFAPDAVMAGTPHLPGTREFFSCLEGHVTIFVAGERYELDAGDVLGFP
                     GNVPHSYRNMDRAASALGVSIVVLAKAGV"
     gene            complement(15747..16565)
                     /locus_tag="VFO53_07265"
     CDS             complement(15747..16565)
                     /locus_tag="VFO53_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8876111.1"
                     /translation="MNTPGTDTTFAGSIPHLYERHLVPLIFAPYASDLAARVASRSPS
                     RVLEIAAGTGVVTRALASILPPSAVIVATDLNQAMLDQAKAIGTSRPVEWRQADAMRL
                     PFPDASFDVVVCQFGAMFFPDKPAAFAQARRVLRNRGAYLFNVWDAIEENEFADSVTQ
                     ALATCFPDDPPRFLARVPHGYHDASRIAHDVALGGFERPPLIDIVTERSRADSPQAPA
                     VAYCQGTPLRSEIEARDPSRLTEATDAAASAIEQRFGSGVVDGKIQALVVVAER"
     gene            complement(16570..17247)
                     /locus_tag="VFO53_07270"
     CDS             complement(16570..17247)
                     /locus_tag="VFO53_07270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8876112.1"
                     /translation="MKHPFDNIVWNALAGPHADHATGTGEARRYAPGFSPIVGFADPE
                     RPNVDALAPFCEPGEAFYCDGWSGAPPTGWRIDAEEIMLKMIFDAPMPANDDAPDAIR
                     LGPEHVAQVLELVALTHPGPFGPRTIELGEYFGCFENGRLVAMAGERMRAGSLREISG
                     VCTLPDFQGRGLACRLMNKLIRREMERGETPCLHVMRDNRKAARLYEHMGFRLHRESI
                     VRVISKG"
     gene            17457..18206
                     /locus_tag="VFO53_07275"
     CDS             17457..18206
                     /locus_tag="VFO53_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET8876113.1"
                     /translation="MPAKQRPDPRRRRLLAAGGGLVLGTSLGARGALAQANAGTKTPI
                     GVIGSGHIGSTVGGLWVKAGHPVLFSSRHPDELQDMVEKLGTLAKAGTVAEAIAFGDA
                     LLIAVPYAALPRIGEDNRAALKGKVVLDACNAVASRDGDIAAEVERNGIGVTSQKYLP
                     GTHVVRAFNTLSYMILAREANRADPRLAIPVAGDDAAAVRMAEGLVRDAGFDPVAVGK
                     LADASRFQRGGPGYGQNVSAAELKRTLSLAP"
     gene            18203..19486
                     /locus_tag="VFO53_07280"
     CDS             18203..19486
                     /locus_tag="VFO53_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8876114.1"
                     /translation="MTFLHWRSFALPVTPGERAAALWSFAYFFTLLAGYYVLRPLRDQ
                     MGIAGGVKALPWLFTATFVALLVAQPLYGALVARLPRTRFIPIVYHFFALNLAIFWLL
                     LTLGISTTLVARVFFVWVSVFNLFAVAVFWSFMADLFTSEQGKRLFGFIGAGGTAGAL
                     LGPVITIGLSVPLGPANLLVVAIVFLELAVLCAHRLERTTASHPASPVRERRLGGSAF
                     AALPALFRSPYLLGVGAWVSLLSFGATILYFEQANIVSATVHGAGAQTRIFASIDLAV
                     GLLTLATQVFATGRVLERFGVGAAAAALPAVYVAGFAVLAVVPTLAVVLVFQVVQRWM
                     NFAIANPARQVFFTVVDREDKYKAKNLIDVVIYRGSDALYGWVFDSLQMIGLKLAGIA
                     LWSLPVVAGWLVLSVALGRTQERRAAQDIAVAQGD"
     gene            19489..20385
                     /locus_tag="VFO53_07285"
     CDS             19489..20385
                     /locus_tag="VFO53_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8876115.1"
                     /translation="MIGLTRREFVALVASSVFTSRVVGETSNSPMIARPIPHSGERLP
                     AVGLGTASVFDRDADDTRRSAAQVVRALIDGGARLIDTASTYGDAELVLGKVITAGSV
                     RDKLFIATKLEEPDESELERSLTRLRTRKVDLLQLHNVRDPRQSLARFRAWKAQGICR
                     YVGITSTYHSDFDAVFAVLGREKPDFVQIDYSIDNRVVEARILPLAADVGAAVLTALP
                     FGRGRLFRAVRDKPIPDWARPFAGSWAVFFLKYLLADERVTAVIPGTTSAAHMADNLG
                     AMHGALPDSAQRKRMVSFIAGL"
     gene            complement(20513..21148)
                     /locus_tag="VFO53_07290"
     CDS             complement(20513..21148)
                     /locus_tag="VFO53_07290"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET8876116.1"
                     /translation="MDTLLHHLCTELVLNFGAHTILLYGSRADGTENEFSDYDIAAFA
                     DVPVSKRDTRVVDGKFLDVFVYPEAVLRKPTKEHLTLRGSRILSQRNVEATHFLAALD
                     VIFRTGPEPLPADEIEARRSWARKMGLRMRRSDIEGNFRRVWLLTALLEDYFALRGMW
                     YQGPKKSFQWLQASDFETYAAFEEALKPASPFDAIDRVVERVVAVTEHSPA"
     gene            complement(21222..21617)
                     /locus_tag="VFO53_07295"
     CDS             complement(21222..21617)
                     /locus_tag="VFO53_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET8876117.1"
                     /translation="MIARTASCSCGQLTAVAAEDPIRVSICHCLACQRRTGSVFGAQA
                     RFPRRSVTTSGKSKAFVRIGDSGKEATFHFCPDCGSTVYYMLEGQEDVYAIPVGAFAD
                     PQFPSPTRSVYEARIHSWVGVPRDIEHLE"
     gene            complement(21636..21956)
                     /gene="sugE"
                     /locus_tag="VFO53_07300"
     CDS             complement(21636..21956)
                     /gene="sugE"
                     /locus_tag="VFO53_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HET8876118.1"
                     /translation="MAWLLLVLAGLFEVGWAIGLKYTDGFTRLWPTIGTVLAMIISLG
                     LLGIAMKSLPVGTSYAVWVGVGAVGTAVLGIVLLGEVANAGRIVSLGLIIAGIVGLKI
                     ATPG"
     gene            complement(22183..22590)
                     /locus_tag="VFO53_07305"
     CDS             complement(22183..22590)
                     /locus_tag="VFO53_07305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET8876119.1"
                     /translation="MANVTRCIFCEIVARHVPATIVFEDNDHLAFFPLEHINPGHVVL
                     IPKSHIDYLFDMEPPRYQALWAAAAQLAPGLRSVTSAKRIAIAVEGFAVPHVHIHLVP
                     VYAGDELNPSRAKPLPAAEAEHLAGLLREVFGA"
     gene            complement(22950..23594)
                     /locus_tag="VFO53_07310"
     CDS             complement(22950..23594)
                     /locus_tag="VFO53_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015158066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HET8876120.1"
                     /translation="MLRAASYVFDLDGTISDPAVGIGRSLTYALQYFGYPLISERAVS
                     RFIGPPLDETFRSIVDDATPQRIAALVSKYRERYAAIGFSENVIYPGILEALRALVRT
                     GAVLGLCTSKRADFADRILRLFGIREHFRFISGGDIGISKREQLGALLATESIDGSFI
                     MVGDRAVDILAARANGLAAAGVLWGHGSLEELRAAAPEFLVEFPHELVEGVCAA"
     gene            complement(23604..24059)
                     /locus_tag="VFO53_07315"
     CDS             complement(23604..24059)
                     /locus_tag="VFO53_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8876121.1"
                     /translation="MENEVLVRTARPSERNALEALQLRASLNNPGDREALLANPDAIA
                     LSSEQIARGRVFVVEVARSTKGFAAILRRDDGNLELDALFVEPTEWRRGFGSRLLDRC
                     AQEARTGGAKAIHVVGNPHAEGFYMARGFEILGKRMTRLGVGILMRKVL"
     gene            complement(24183..25229)
                     /locus_tag="VFO53_07320"
     CDS             complement(24183..25229)
                     /locus_tag="VFO53_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HET8876122.1"
                     /translation="MPLETAGSKTAVETTEMKKWIFGAIALLAVAVVGVMAFQVATGL
                     RPSRPVGFQVVHVPDRSGPPLSVAVWYPTEAGARPALLGLTVQFVANDAPVAGTSLRL
                     VIISHGSGGGPGSHADTALALASEGFVVAAPMHTGDNYADQSAVGTPDWLVARTRHVP
                     VVIDFLLTTWPDHARIDPSKIGMFGFSAGAFTALTTIGGTPDLALIAGHCATTPEFVC
                     RLWKPVGDDVPMAQSFSHDPRVKAAVIAAPGYGFTFVPNGLAKVTVPVQIWSGAMDNN
                     VPTASNAAPVQAALGARADLRVVPGAGHFSFLVPCGLIGPPLLCRDAKGFDRKLFHAS
                     FNQEVVRFLQAKLN"
     gene            complement(25297..25599)
                     /locus_tag="VFO53_07325"
     CDS             complement(25297..25599)
                     /locus_tag="VFO53_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876123.1"
                     /translation="MSRNLDLSAEARLHRLEQMRVGIADGQRLLIQRRYSLGAPAFSS
                     DCSRPSRFAGTLSALAFRERLETLYGGDAKLNLRRRGDDTIVAVLDLPLEARQSDD"
     gene            complement(25653..>25928)
                     /locus_tag="VFO53_07330"
     CDS             complement(25653..>25928)
                     /locus_tag="VFO53_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase"
                     /protein_id="HET8876124.1"
                     /translation="FAMASYLLIRHKVRDFAQWKRGYDAHLPKRAAAGLTEKHLLRGA
                     SDANEVVLLFQAEDLRRAKAFAESADLRQTMQNVGVVDKPDIYFLNG"
BASE COUNT         4443 a         8446 c         8713 g         4326 t
ORIGIN      
        1 gcaccgtatg tctgttcggc ggcgcgccgt atccgccgca gtatcgagag ctcgagcgcg
       61 gcgtcgacgt gctcgtcgca accccgggcc ggctcatcga tcacatcgag cgcgggcgcg
      121 tcgatctgtc cggactgtcg atgctcgtgc tcgacgaggc ggaccgcatg ctcgacatgg
      181 gcttccagga agacgtcgag cgcatctgct cgcgcgcgcc ggccgcgcgc cagacggtgc
      241 tgttttcggc aacgttcgac ggggccgccg gccggctcgc ggcggagctt tcgcgcgacg
      301 cggtgcgcat cgacgttcgc accacccgca ccgcaccggt cgcgatcgag cagcgcgtgc
      361 atttcgccga cgaccgcgcg cacaagtacc gcatcctcga ccatctgctt gccgacgtcg
      421 gcaccgggca gagcatcgtg ttcatcgcga ccaggcgcga tgccgagatg ctggccggca
      481 agctgcagga cgagggacac acggccgcgg cgctgcatgg cgacatgaat cagcgcgagc
      541 gcgaccgcac gctgacgcgc atgcgccgcg gcggggtccg cacgctggtc gctaccgacg
      601 tcgcggcgcg cgggctcgac gtgccgggcg tcacgcacgt catcaacttc gacctgccgc
      661 ggcaggccga gaactacgtg caccggatcg gacggaccgg gcgcgcgggc gcgtcgggcg
      721 tcgccgtttc gctggcgaac gccaccgagc gcggaacgct gcgccagatc gagcgttaca
      781 cgggtgcggc gatccccgcg cacgtgattc cggggctcga gccacgcgtg cgcacgcaaa
      841 atggcgtgcc gaagcggccg tacggcggcg gtatgatgaa gggcaggggc gccgatcgtc
      901 gcaatcctcg cgggcggccc ggtcgctgag gcgaaaggat ccgccggatg tacgcacaga
      961 tggtcgaaac gggcttgaag aaggtccagg acgccgctga catgacgccc gaggacgtcg
     1021 cgttccaggc ccggatcgac gccgacgtca agatcgagcc gaaggacgcg atgccggagg
     1081 cgtaccggcg cacgctgatc cgccagatcc agcagcatgc gcattcggag attgtcggca
     1141 tgctgccgga gggcaactgg atcacgcggg cgccttcgtt gcagcgcaag gcgatcctga
     1201 tggccaaggt gcaggacgag gccggacacg gcctctacct gtactgcgcc gccgagacgc
     1261 tcggcgtttc gcgcgaccgg atgatcgacg atctgcacgc cggcaaggtc aaatactcgt
     1321 cgatcttcaa ctacccgacg ctcacgtggg cggacatcgg cacgatcggc tggctcgtcg
     1381 acggcgcagc catcatgaac cagataccgc tgtgccgctg cagctacggg ccgtacgcgc
     1441 gcgcgatgat ccgcgtgtgc aaggaggaat cgtttcacca gcggcagggc ttcgacatca
     1501 tgatggcact gtgccgcggc actccggaac agaaggcgat ggcgcaggac gcactcgatc
     1561 gctggtggtg gccgtcgctg atgatgttcg ggccgccgga cgcggcgagc gtgcacagcg
     1621 cgcaatcggc gcgctggaag atcaagctgc tgtcgaacga tgaattgcgc cagcgcttcg
     1681 tcgaccagac ggtgccgcag gcggactatc tcggcttgcg cataccggac gacgcgctcg
     1741 tcttcaacga ggagcgcggg cactacgact tcggcgcgat cgactggaac gagttccacg
     1801 aagtgctgaa gggccacgga ccgtgcaacc gtgaccggat gcgggcgcgt gtcagcgcgt
     1861 gggaagacgg cgcctgggtg cgcgaagcgg cgctggcgca cgcggacaag cgcgcggcgc
     1921 gcgagcgagc ggcaacgcag ccggcccccg gactgcaaag gcagatggag gccggatgag
     1981 cagcgaatgg ccgttgtggg aagtgttcat ccgctcgcag cacgggctcg cgcacaagca
     2041 cgtcggcagc ctgcacgcgg tagacgcgga aatggcgatc aggaatgcgc gcgacgtcta
     2101 tacgcggcgc aacgaaggcg tgtcgatctg ggtggcgcgc tcgtccgaca ttcacgcatc
     2161 ttcgcccggc gatcgcgcgg cgctgttcga gccgtcgaac gacaaggtgt accggcaccc
     2221 gacgttcttt ccgatgcccg atggagtgaa gcatctttag cccgctttgc gactacgcgc
     2281 tgcgccttgc cgacaacgac ctcgtcctcg cacagcggct cggcgaatgg gtcggcaagg
     2341 gcccggtgct cgaggaagac atcgcatcga cgaacgtcgg gctcgatctg cttgggcagg
     2401 cgcggctgtg gcttgcttac gccagtgaaa tcgacgacgg ctccgaggat cgattcgcgt
     2461 actttcgcga cagcaccgaa ttccgcaacc tgctcctggt cgagcagccg aacggtaact
     2521 atgccgacac gcttgtccgc caatacctgt tcgaccagtg gcacgtgctg ttgcttgcgc
     2581 aactgtcgac gtcgcgcgac gcccgcatcg cgagcatcgc gacgaaggcg ctgacggaag
     2641 tgcactatca cgtcgaacgc tctgccggct ggatgatccg cctcggcgac ggcagcgatc
     2701 tgtcgcacgc gaggatgcag gcggcgctcg acgcgctgtg gatgtacacc ggcgagatgt
     2761 tcgccgtcga cgccaccgag cgcgcgctca tcgacgccgg catcgctgcc gatgtcgcag
     2821 cattgcacgc gccgtggctt gccgcgctgc agccggtgct cgccgaggca acgctcacga
     2881 tgccggacga cgcgtggatg cagggcagca gcggtcgcgg cggcaagcag ggcgtgcata
     2941 ccgagcatct gtcacggatg ctcgccgaga tgcaggtgct gccgcgcacc tatccggacg
     3001 cgcactggtg acgtcgatgc tcgaagacgg gccgcatccg atgcaaggtt ccccggatcc
     3061 tcgcagcgtg tggcgcgccc tcggcaccgt ggccgatccg gagcttccgg cggtgtccat
     3121 cgtcgagctc ggcatcgtgc gcgaggttgc atgcaccgca gacgaatgca tcgtcacgct
     3181 gacgccgacg tattcgggct gccccgcgac ccgcatgatc gaagccgatg cgaaggccgc
     3241 cgtggcgagc gtgaccgatg cgcgcgtgcg cctcgttacg gcattgacgc cggcctggtg
     3301 cagcgactgg atcgcgcccg aggcgcggcg caagctggcc gcgtccggaa ttgcgccacc
     3361 gcgccacggc ggagcgcagc cggcgatccc gatcgcggtg gatcgcggcg cgatccgctg
     3421 tccgaactgc ggctcgcgac gcaccgagga gattgcccgc ttcgggtcga cggcctgcaa
     3481 ggcgcagcat cgctgccgcg actgcctcga gccgttcgac tacttcaagc agcactgacc
     3541 gtccgatgtg caggccgtga gcaggttcca tcggctgccg atcgcgcgca tcgaccgcga
     3601 aacgcgcgat gcgatcacca tcacgttcga cgtgcctgcg tcattgcggg agcagttccg
     3661 cttcgacgcg gggcagcacc tgaccctccg ggcagagatc gatggcgagc ccgtgcgccg
     3721 ttcctactcg atctgtgcgg ccgaaggcga cacgctgcgc atcgccgtga agcgcgcgcc
     3781 gggcggccgc ttttccgcgt gggcgaacga gtcactgcgc gcgggcacgc acatcgacgt
     3841 gacgcaaccg atgggccgct tcaacgtcgt gcgcgatccg cgcgaatcgc gccactatct
     3901 cggcgtggcc gcgggcagcg ggatcacgcc gctcctgtcg atgctcgcga cgtcgctcga
     3961 ggccgagccg cgatcgcgct tcacgctgat ctatgccaac cgctcgtctg ccagcgtcat
     4021 gttccgcgac gagctcgccg cgctcaagga tcgccacctg tcgcgcttca acctcgtgca
     4081 cgtgttgtcg cgcgagccgc aggacatcga gcttttgcat ggccgcgtcg atcgcgcgaa
     4141 ggccgacgcg ctgttcgagc gctgggtgcc gatcggcgac gtcgattacg cgttcgtctg
     4201 cgggcccgaa ggcatgatgg atgcggtccg cgcatcgctc gtggcgcacg gattggccga
     4261 gcagcgcatc cgcatcgagc gcttcgcggc gagcattccg aagcacacgc acgtggcgcg
     4321 gccgctgccg gcggcggcac gcaccgagtg cgaagtcacc gtcgtcatcg acgggtcgca
     4381 gcgcactttt cttcttgaaa agggccgcga gaacatcatc gacgcgggac ttcgcaacgg
     4441 catcgagctg ccattctcct gcaagggcgg cgtgtgctcg acgtgccgct gccggctcgt
     4501 cgccggcgag gtggacatgg acgtcaactt cgcgctcgag gattacgaag tggcgcgcgg
     4561 gttcgttctc gcatgccaga gctatccggt caccgacagg atcaccgtcg atttcgacca
     4621 gtcgggcacc ggataacggc gtaaagctgg tgcatggttg catcccggtg ccccgcacgc
     4681 gctgttgttg cgtgctagag taaacgcatc acggaatcga agagagccgt ctcgtgacgc
     4741 atccgttttt cgagcgacac cgcgcaaccc tcgagcgcgc tgtcgaagcc attgcgacgc
     4801 gtggcttctg gacgccgttt cccgagtcgc cgagtcccaa ggtctacggc gaaggcacgg
     4861 cggaagcagg cagggccgcg ttcgacaagc tcgcgaacca gcgctttgca ttgacgcaac
     4921 cggcgacgat cggccaggtc ggcggcgagc gctcgccgta cggcatccgt ctcggcatta
     4981 cctatccgaa gccggatgtc gacggtctca tgtcggccgt cgaggcggcc gaggcgagct
     5041 ggcgcaaggc cgggccgcaa gcgtgggtcg gcgtgagcct cgagattctc gatcgcatca
     5101 acaaggcgag cttcgagatc gcgcacgccg tcatgcacac caccggtcag gcattcgtta
     5161 tggcattcca ggcgggcggt ccgcatgcgc aggatcgtgg cctcgaggcg gtcgcttatg
     5221 catgggacca gatgcgccgc gtgccggcgc aggccgcgtg ggaaaagccg cagggcaagg
     5281 gcgaacccgt gcggatggaa aagcgctttc gcatcgtgcc gcgcggcgtg gcgctagtca
     5341 tcggctgcag cacgttcccg acgtggaacg gctatccggc gctgttcgcg agtctcgcca
     5401 ccggcaacgc cgtcatcgtg aaaccgcatc cgaacgcaat cctgccgctc gccattaccg
     5461 tgcgcatcgc gcgcgacgtt ctgcgcgacg ccggtttcga tcccaatgtc gtcacgctgg
     5521 ttgcacacga ggcgggcgac gacatcgcgc agtcgctcgc actgcgcccc gaaatcaaga
     5581 tcgtcgattt caccggcagc acggcgaacg gccgctggct cgaggagcat gcgcgccagg
     5641 cgcaggtgta cacggagaaa gccggcgtga accaggtgat tgtcgattcg gccgccgact
     5701 tcaagggcct cgtgcgcaac ctcgcgttct cgctctcgct ctacgccgga cagatgtgca
     5761 cggcgccgca gaatatctac gtgccgaaag ccggcatcga caccgccgaa ggtcatcttt
     5821 cgttcgatga cgtcgcggcg gcgctcgcgc agggcgtcga gcgcctgctc gccgacgaca
     5881 cgcgcgcggt cgaggtgctc ggcgcggttg ccaacgacgg tgtgctgcgc cggctcgagg
     5941 ccgcgcgctc gctcggcgac atcgcgctcg acacgcgcac gatcacgcat gcgcagtttc
     6001 ccgatgcagt cattcgcacg ccgctcgtcg tcaagctcac acgggccgac cgcgcgacct
     6061 atctcaacga atggttcggt ccgatcgcat tcgtcgttgc aaccaacagc accgacgaca
     6121 gtctcgccat cgcgcgcgat gcggtggtcg ggcacggtgc gctcacgatg tcggtgtact
     6181 cgacgagcga cgacgttgtc gcccgcgcga tcgaggtcgc cgaggatgtc ggcgtcgccc
     6241 tgtcgatcaa cctgacgggc ggcatcttcg tcaaccagtc tgcggcgttc tccgatttcc
     6301 acggcacggg cgccaatccc gccgccaacg cggcgctgac cgatgccgcg tatgtggcga
     6361 gccgctttcg cgtggtgcag catcgcatgc atgcaccgca ggcgcaggag gcctcttgac
     6421 ttacgaacag atacgattcg aatgcggcga tggcatcgcg cgcatcacgc tcgaccgtcc
     6481 cgatcggctc aacagcttca cgacgcagat gcacgaggag ttgcgcgacg cgctgcggcg
     6541 ggtcgcgacc gatccctccg cgcgcgtgct gctgctgact ggcgccggcc gcggcttctg
     6601 cgccggccag gatctggccg accgcgccgt cgcccccggc gatgcacctg tcgacctcgg
     6661 cgcatcgatc gagaaccatt accggccgct cgtctgcgcg ctgcgtgacc ttccgctgcc
     6721 cgtggtgtgc gcggtgaacg gcgtcgcggc gggcgctggc gcgaacgtgg cactcgcgtg
     6781 cgatctcgtc atcgcgacgc gctcggcgag cttcatccag gcgttctgcc gcatcggcct
     6841 cattcccgat gcgggcggca cgttcttcct gccgcgactc gtcggcagcg cgcgcgccat
     6901 gggcctggcg cttcttggcg acaagcttac tgccgagcag gccgagcagt ggggactcat
     6961 ctggaaatgc gtcgacgatg atgcattcac gtccagcgtc gatgcattgc tcgctcagct
     7021 tgcgcaggcg ccgaccggcg gcctcgccgc gatcaagcgg acgctccatg cgtcggccgg
     7081 caacacgctc gacgcgcagt tgacgctcga gcgcgacacg cagcgcgtgc tcgggcaaag
     7141 cgccgattat cgcgagggcg tgaccgcatt cctcgcgaag cggccggcac atttcaccgg
     7201 ccgctgacca tgaccggagc gcccatcgcg cgcgatgcga cggtggccgt cgtcggcgcc
     7261 ggaacgatgg gctgcggcat agcgcaggtc gccgcgcagg ccggacaccg cgtgcggctg
     7321 ttcgacacgc gactcggcgt cgccgagaag gcgcgtgcaa gtatcgggca gacgctgaac
     7381 gcgcttgccg cgaagggcaa gctcgacgcg ggtgctgtag aggccgcggc cgcgcggatc
     7441 gcggtggtgc acgcgctcgg cgattgcgtg agcgcacggc tcgtcatcga ggcgatcgtc
     7501 gaggatctcg ccgaaaagcg ttcgctgctg cgcgagctcg agaaggtcgt cacggcgtcg
     7561 gccatcctcg cttcgaacac gtcggcgctg tcgatcacgg cactcgcgag cgggctgcgc
     7621 tttcccgggc gcgtcgtcgg catgcacttt ttcaacccgg tgccactcat gcaactggtc
     7681 gagatcgtga gcgggcttgc cacggacgac gcggtggccg atacggtcgc ggcaaccgcc
     7741 gctgcctggg gcaagcagcc ggtgcgcgcg acgtcgacgc ccgggttcat cgtcaaccgg
     7801 tgtgcgcgtc cgttctacgg cgaggcgttg cgcctgcttg ccgagcgcgc cgcggatgtg
     7861 ccgacgctcg atgcgctgat gcgcgacgca ggcggcttcc gcatgggcgc attcgaattg
     7921 atggacctga tcggcaacga cgtcaacttc gcggtcacgc gcggcgtatg ggctgcgtgc
     7981 taccacgatc cgcgctatgc gccgtcgatg ctgcaggagg agcgcattgc ggcgggatac
     8041 ctgggccgca agagcggacg cgggttctac gattacgcgg aagggtcgag ccggcccgcg
     8101 ccggcgagcg tttcggcgca gccggcaccc aagcggctgg tggtgcgcgg cgagcttggc
     8161 gccgtttcac cgctcgtacg ccggttcgac ctggccggca ttgcggtcga gcaggcgccg
     8221 gcgcgcgacg cgttcgccga cggtgcaatc gaaatcggcg atgcggtgct cgcgttatcc
     8281 gatggccgca cggcgactgc gcgtgcggct gccaccgggg cgaagaacct cgtgctgatc
     8341 gatctcgcgc tcgactacgc acgctgcaac cgcatcgggc tcgcgcgcgc cgacacctgc
     8401 ccgatcgctg cgttcgagac ggccgcgggt gcgttgcagg ccgccggggt tgccgcgtcg
     8461 ccgctcgacg atgtcgcggg gctggtcgtg ctgcgaacgg tcgcagcgct tgcgaacgag
     8521 gcggccgacg cggtgacgca cggcgttgcg tcggcgcatg acgtcgacgc ggcgatgcag
     8581 aagggcgtca actatccacg cggcccgctg cgctgggccg atgaaatcgg cttgcggcgc
     8641 gtgcgcaacg tgctcgtgaa cctggcggcg cactatggcg aggatcgtta tcggctgtcg
     8701 ccgttgattg cgcgtcgcta tgccgccggc gcaccgcttg ccgacggcgg atgaggcagg
     8761 cgcgatgaac gatgacgcga acccgggtcg cgagattgcg caatccctcg cggaacgcgt
     8821 cgccgcgcgc atgttcgacg ccgatcgcgc gtcgcgcgca ctcggcatgc acatcgaatg
     8881 cgtcgcaccc ggacgcgccg agatgacgat gaccgtgcgc gacgacatgc tgaacggcca
     8941 cgcgatctgc cacggcggct acatcttcct gcttgcagac agcgcgttcg cattcgcatg
     9001 caacagctac aaccgcaaca ccgtggccca gggctgcacg atcgactatc tcgcaccggc
     9061 gcgcgccggt gacgtgctgc gcgcgaccgg cgtcgagcgc agccggaccg gacgtaccgg
     9121 cgtctacgat atcgacgtca cgaaccagca gggcacgacg atcgcgctct tccgcggaaa
     9181 gagctatcgc gtcgacggca acatcgtcga cgagcgctga acgtcggccg gaaaggacgg
     9241 caatgccggc gacgtctccg cgcgaggctg atctcgagcc gatcgagacc gccagccgcg
     9301 acgagctcgc ggcgcttcag gtgcaacggt tgcgctggtc gctgcgccac gcgtacgaca
     9361 acgtggcgca ttaccgcgct gcattcgagc gggcgggcgt tcatcccggc gacctcgaaa
     9421 cgctcgacga tctcgcccgc tttcccttca cgaccaaggc cgatcttcgc gacaactatc
     9481 cgttcggcat gtttgccgtg ccgcgcaccg acgtcgttcg cgtgcacgcg tcgtccggaa
     9541 cgacgggcaa cccgacggtc gtcgggtaca cgcgcaacga catcgagacc tgggccgcgg
     9601 tgatggcgcg ctccatccgc gccgccggcg gccgggcggg cgacatcgtg cacatcgcct
     9661 acggctacgg acttttcacc ggcggcctcg gcgcacacta cggcgcggag aggctcggct
     9721 gcacggtcgt gccgatgtcg ggcggacaga ccgcgaagca ggtgcagctc atcaacgact
     9781 tccggcccga catcatcatg gtgacgccgt cgtacatgct cgccatcgcc gaggagatgc
     9841 agcggcaggg cctcgatcca cgcgaatcct cgctgtccat cggcattttc ggtgcggagc
     9901 cgtggacgcc gaccatgcgc acggcgatcg aaaccatgct cgacatcgat gcgatcgaca
     9961 tctacgggct gtcggaagtg atcgggccgg gtgtcgcaca ggaatgcatc gagacgaagg
    10021 acgggctcac cgtctgggag gaccacttct accccgagat cgtggatccg gtaacgggcg
    10081 ccgtgctgcc cgaaggcgag aagggtgagc tcgtgttcac gtcgctcacc aaggaagcga
    10141 tgccggtcgt ccgctatcgg acgcgcgacc tgacacggtt gttgcccggc accgcacgca
    10201 cgatgcggcg catcgagaag atcaccggtc gctgcgacga catgatcatc attcgcggcg
    10261 tcaacgtgtt tccgacgcag atcgaggagc tgatcctgcg cgacccgatg ctggcgcccc
    10321 actaccaggt cgagatcgcg cgcccgcgca acctcgacga aatgaccgtg ctcgtcgagc
    10381 gcgcgccagc cgccggcggc accgatgccg aggccgcggg cgagcggctc gcacggcgga
    10441 tcaaggatct cgtcggcgtg agctgcgccg tgcgcgtgac gcaaagcggc ggcatcgaac
    10501 ggtcgctggg gaaggcgaag cgggtcgtcg acaagcgtcg ttgacgcccc gcgtcgatca
    10561 acgccgctag cggcgcctcc cggcgatgcc gcttaccgcg acaagcgcgc ccagcagcag
    10621 cagtgcgagc tcgctcatcg tcgggatcgg ctgcaccgtt cccggcggcg gcgggacgcc
    10681 ccccgcattg acgacctgct gcaccgcggg cgaagtgctt gccgcattgc tggcgtcgcc
    10741 cgagtaaacg gcggtgatcg tgtggctgcc ttgcgtcagt gccgaggtcg tgagcgccgc
    10801 tgtcccaccg ccgccgagtg cgatggtccc gaggctgctc gctccgttga agaactgcac
    10861 gctgccggtg gggctcgcgc cgctcaccgt cgccgtgaac gtgaccgcct ggccgagcgt
    10921 cgacggattc agcgacgagg cgagggcggt ggtcgtggcg ccggcaggca tcgcattgac
    10981 gacctgcgtg agcacggccg acgtgctcgt cgcgttgttc gcatcgcctc catacacggc
    11041 ggtgatcgcg tgcgagccct gggtcaatgc gctcgtcgcg agcgcggcaa cgccgcttcc
    11101 gttcaacgcg acggggccgc cgaggttcac gacgccgttc ttgaactgga cgctaccggt
    11161 gggcgaattc ccggtgacgg ttgcggtgaa cgtgaccgat tgaccgaccg tcgacggatt
    11221 cagcgacgac gcgacgcttg tcgacgacgc gccggagaca gctgctgcgc acgccgagaa
    11281 ttccgacgta tcgccacccg cgctcgtggc ggtggacgtg atcacgccct ggccgagcgg
    11341 cacagcgaat gcaagcggtc cgaagctggc gttgccgctg ccgtcggtcg ttacatcgat
    11401 ggcgccgatg aagctctggc cctcgccgtt ccccgacgca tcgcatgttg cattggcgaa
    11461 gaactcgaga tgcaacggcg cgcccgcatt gctgttgatc gtgccggcga tcgtcgccgt
    11521 tcccgccgcg atggccacgg aagcgatcac cggataattc tggccatgat tgcccggcac
    11581 gacgtcggcg tcgccggcat cgttggcgag cggcgtgccg gagtgcagca gcgtgatgcc
    11641 cagggccgtg ttcgagtgaa tcgagttctg cgtgatgcgg ttgccgaaca ggttgatgaa
    11701 cgaatcgatg ccgctgttga cgttgaacgc gatgacgttg ccttcgcccg cgcccgatcc
    11761 gccgatcgtc gatccattgc cgtcgacgct gatgccgccg aagttgccgt tcgggagcgg
    11821 agtcgtgcca tcgcgcgccg tgccgatcag attgccgcga aggacggcgt tgtcgagaag
    11881 gttgatgccg ccgcccggat tccccgacac gagattgcca agaacgctcg cattggccat
    11941 gaggtcaagc ccttcgcccg agccgagagc gacggtcccg gtgacatcgg tcccgatata
    12001 gttgccctgg atcagatggc cgctcgtgct cggaaacggc aggatgatgc cgccttgcgc
    12061 attgccggcg atcaggttgc ggtcggcagg attcggtccg ccgatcgttg cgttcgccgc
    12121 ggatccctcg atgcgcacgc cgaacccgcc aaagctgccg gacgcggtcg ccgtaccggt
    12181 cgcgttcgtg ccgaggaagt tgccagtcac cgtgagattg ctcgactgac cgtcgatctt
    12241 gtcgttggac gaaccattga tgacgaggcc gcgaaccttc gatccgtcgc tgcccggtgc
    12301 gaagaacagc gacacctgcg tgccggtgag ctcgatctga agcacggcgt tgatgccggc
    12361 attcatcgga ttcgtattgg ccaccgagcc gggctgcgtg tagccgtcga tgaccacggg
    12421 gctcgtgatg agcggcaaca gcgacgtcag cgtgatcgta tggacgccgg cgcccgggat
    12481 gctgaacacg atcgtgtcgc cgccgacggt gccgttggcg tcctgcagcg cctggcggaa
    12541 gctgccggca cccgagtcgt tcgtattgct gaccgtgaac gttgcagcga gggcgggagc
    12601 cgcaaacagc gcgacgagca acgcgagaca atacgcgacg acgggtgacc ggtacatgcg
    12661 cagcactcct ggttacccgg gctggggtgg cgcattgtgt cgtgttgtac aagcttgtca
    12721 agcgattttc gcgccggtgc tgcaagtgat cagaccacgc ccgcgacccg caatccagcg
    12781 aatggcggcg cgccggtcgt tgcgcgccac ggtttcgcct gaaaagcgcg catcatgggg
    12841 aacttcccgg acccgtacaa cgcgcggctc acgccagctt gacgcagggt cgctcaggtg
    12901 ctgggtgtcg accggcaggg ggattgcatg aacgacgaaa agattccggt acgcgtaacg
    12961 agcagcggac agacgctcga ggtggtcgtc ttcgacaagc ggctcgacgt gattcaggtc
    13021 gtgctcggag aaggcgtgca cagcgtgcga tgcgacctga ctcccacccg caacggccta
    13081 gcctacgtcg gcaacgcgag gggacgcgag atcgtgtacg agcgcacgcg cgaacaggtg
    13141 aaggcggacc tcgctcgtgc ggccggcttt cgcgacttca agcgatagcg atcaaggcgc
    13201 gcggccctgc gcagagcccg gcacgacgga aggagcagct cgctccacca gcaccgaatc
    13261 cccgaactcg tgcgtccggt agccgtgctg ttccagctcg cggtcggcgt cacgcagcgt
    13321 cgcggcggac acgaacgcgc ccagcagcgc gtaatggctc gtccccggct cgtgcgtccc
    13381 cgaaagcacc gcgtcgacga cgcgtagccg cgtcccgggg acgatgcgcg tcgtggcgac
    13441 gccggcgccc ggacgaacga ggccgatcac cgtggcggcc gcctccagcg cacggacgac
    13501 ggtcgttccg accgcgatca cgcgtcgctt gcgttcgcgt gtgcgcgcaa cggcgtccgc
    13561 agtcgagcga ggaatgcgat aaggctcgtc gagcggaagc agcgcatcga gcgcttcgtc
    13621 gccggtcgac gaaatgccgg ctgcgtgcgt gagcgtcgcg aattcgatgc ctcgccggcg
    13681 catctcggca agcagcgccc agtcgagcgc gaagcccgcc gacggggcct cgaacgcggc
    13741 gggcgtgccg gcgaacgccg tccatacgtc ccacagctcg agtgcgggac gcatgtaggc
    13801 gtactggatg gcccgcccat gcgacgcgag accgcgccat atcgcttgcg ccgtgccgtc
    13861 gaagcgcagc gccgcgaagc gcggatgccc gagcagaccg cgcaccgtcg cgcgaagcgg
    13921 ccccagcgtg agcacatcgc cgggcgcgag ctcgggcggc gggcggcgat cctcggtcgg
    13981 cgtgcggtgg tcgcctgcgc cgaagatcac cgcggcaaat tcgcggacgt cctcgggatc
    14041 gagcgagccg cgtcgtgcga ggcgcacttc gatgttctcg ccgctcgcga cgtgcacgcc
    14101 gcaaaggctt gcgggcagcg tcgcggcatc gttggcaatg acgagatcgc cacgcgaaag
    14161 cagcgccggg aacgcgcgcc gcggccaatg cgcgatcctg ccggccgcgt cgatggcgag
    14221 gagtttcgcg tccggcggcc gctggatggg ttcgtgcgcg acgatcatgt cagtcgggaa
    14281 gggccgcccc cgaccgactg acccgtccct tggggggcag cgagctcagc gagcggggag
    14341 ggacgtttca ttcctcgcgg ctgcgcttgc gctgcgcgag ctcgcgcagc tcggcaacga
    14401 ttgcgcacgt gagctcccgt gccgatgact gcggcgcctt gagcgtcgac ggatcggcgt
    14461 cggggatcgc gagcgcgtgc agcggcgtat ccatgtcgcc gggatcgagt gcaagaacgc
    14521 caatgccttc gtcctgcaac tcgttgcgcc agattgcgct catgtgccgc agcgccgcct
    14581 tcgaggcgcc gtacgcgccc cagtgcgaat acggcgtcag cgcagcatcg ctcgaaatgt
    14641 tgagcacgac cgcgccgcgg ccctccgcag ccgaagcgcg tagcgcgccg agcagcgtcc
    14701 tggtcaggcg gaacggacca agcaggttgg tcgcgagcgc cgtttcgaag tcttcgcatt
    14761 cggtgtcggc gagcaatgcg agcggcaccg ggccgagact cgaggcgttg ttgacgagca
    14821 cgtcgaggcc gccgagccgc ccgagaatct gcagcgcgat cggatgaatg ccggccttcg
    14881 tcgatacatc gccgacgatg ccggccgctc ccgggatttc gcgtgcgatc cgctccacgc
    14941 tgcccggcgt gcgcgcgacg aacgcgacgc cggcaccgcg cgcgctccac tcgcgaacga
    15001 gcgcgaggcc gagtcccgac gttccgccgg tgatcgcgat gcgcagtccg gcaaggtcgt
    15061 tgcgatgatc gtccgttcga ttcatggtcg tttctccggt tgcatgtctg tgacaatacc
    15121 ggcgcggccg tgctttccaa ggcggccgtc cacgcgcttg gcgaaaggtc atcctgatgg
    15181 acaaaccgcc gctcgtatcg gcacatctcg cgcgaaacct cgcggcgttg cgccaggtcc
    15241 gctcactcac gcaggaggcg ctcgcgaaat cggccggcgt gccgcgctcg acgatcgcca
    15301 acctcgagtc gggtgagggc aatccatcgc tggccgtgct cgtgaaagtg gccgaggcgc
    15361 tttcgacgtc gatcgacgag ctagtcgcgt ctccgcgcgc gaaggttcgg cgctgggcgg
    15421 caaacgacgt tgcatcgcgc cggcggggac gcggcgttac cacgcgcgcg ctcgttccgg
    15481 aaccggtccc cgaggaagtg atggaattga tgcaattcgc gccggatgcc gtgatggccg
    15541 ggacgccaca cctgcccggc acgcgcgaat tcttttcgtg cctcgagggg cacgtcacga
    15601 tcttcgttgc cggcgagcgt tacgagctcg atgccgggga cgtgctcggc ttccctggca
    15661 atgtcccgca ttcgtatcgc aacatggacc gcgcggcgag cgcgctcggc gtttcgatcg
    15721 tcgtgctggc gaaggcgggc gtgtagctac cgctcggcca cgacgaccag agcctggatc
    15781 ttgccgtcga cgacgcccga tccgaagcgc tgctcgatcg ccgaggcggc tgcgtcggtt
    15841 gcttcggtga gacgcgatgg atcgcgcgcc tcgatctcgc tgcgcagcgg ggtcccctgg
    15901 caatacgcaa cggcgggagc ctgcggcgag tccgcccgac tgcgttccgt gacgatgtcg
    15961 attagcggcg gccgctcgaa gccgcccaac gcaacgtcgt gcgcgatgcg ggaagcatcg
    16021 tggtatccat gcggcacgcg cgcaaggaat cgcggcgggt cgtcggggaa gcacgtcgcg
    16081 agcgcctgcg tcacgctatc ggcaaactcg ttctcctcga tcgcatccca cacgttgaag
    16141 aggtacgcgc cccggttgcg cagcacgcgc ctcgcctgcg cgaaggccgc gggcttgtcg
    16201 ggaaagaaca tcgcgccgaa ctggcagacg accacgtcga aggatgcgtc gggaaacggc
    16261 aaccgcatcg catccgcttg ccgccattcg acgggacggc tcgtgccgat cgctttcgcc
    16321 tggtcgagca tcgcctggtt gagatcggtc gcgacgatga cggcactcgg tggaagaatc
    16381 gacgcgagcg cgcgcgtcac gacgccggtc ccggcggcaa tctcgaggac acgcgaaggc
    16441 gaacgcgacg cgacgcgcgc ggcgagatcg gacgcgtagg gcgcgaagat cagcgggacc
    16501 agatggcgtt catagagatg cgggatggag cccgcgaatg tggtgtcggt tcccggtgtg
    16561 ttcacgaagt caccctttcg aaatgacgcg aacgatcgat tcgcgatgca ggcgaaagcc
    16621 catgtgctcg tagagccgcg ccgccttcct gttgtcgcgc atcacgtgca ggcacggcgt
    16681 ttcacctcgc tccatctcac gccggataag cttgttcatc agccggcatg ccagaccgcg
    16741 accctggaaa tccggtagcg tgcatacacc gctgatctcg cgcaacgagc cggcacgcat
    16801 gcgctcgccg gccatcgcga cgagccggcc gttttcgaag cagccgaaat actcgccgag
    16861 ctcgatcgtg cgcgggccga acggacccgg gtgtgtcagc gcgacgagct ccagcacctg
    16921 cgcaacgtgc tcggggccga gccggatggc gtcgggcgcg tcgtcgttcg cgggcatcgg
    16981 cgcgtcgaag atcatcttca gcatgatctc ctccgcgtcg atgcgccagc ccgtcggtgg
    17041 cgcgcccgac cagccgtcgc agtagaacgc ttcgccgggc tcgcaaaacg gtgccagcgc
    17101 atcgacgttc gggcgctcgg gatcggcgaa gccgacgatc ggcgaaaagc ccggcgcgta
    17161 ccggcgcgcc tcgccggtgc cggttgcatg gtcagcatgc gggcccgcga gtgcattcca
    17221 cacgatgttg tcgaacggat gcttcatcga gcaccgattg gtccagggct ccgccgcttc
    17281 ccgtaaacgc gttctgccgc gccgcgacgt cgatcttcac cagctttacg ataccggatg
    17341 ataatcgacg gacgaagaaa cggtccgatg aaatagcagt ccaatggcca ttgttcaccg
    17401 atcgttccag gatgtcggtc agcgcatccc tatacgcagc ggcgaggagg ttttcgatgc
    17461 cagctaagca acgccctgat ccgcggcggc gccgcctgct cgctgccggc ggagggctcg
    17521 ttctcggtac atcgctgggt gcgcgcggtg cccttgcgca ggcgaacgcg ggaacgaaga
    17581 cgccgatcgg ggtcatcggc tcgggtcaca tcggcagcac cgttggcggc ttgtgggtca
    17641 aggccggcca tcccgtcctg ttttcgtccc ggcatcccga cgaattgcag gacatggtcg
    17701 aaaagctcgg cacgctggcg aaggccggca ccgtcgccga ggccatcgcc ttcggcgacg
    17761 cgctgctcat cgccgtgccg tacgccgcgc tgccgaggat cggcgaagac aatcgtgcgg
    17821 cgctgaaggg caaggtcgtg ctcgatgcgt gcaacgcggt cgcgtcgcgc gacggcgaca
    17881 tcgccgccga ggtcgagcgc aacggcatcg gggtcacgtc gcagaagtac ctgcccggca
    17941 cgcacgtcgt ccgcgccttc aacacgctgt cgtacatgat cctcgcgcgc gaggccaatc
    18001 gcgccgaccc gcggcttgcc attcccgttg ccggtgacga tgctgccgcc gtgcgcatgg
    18061 cagaaggcct cgtgcgcgac gccgggttcg acccggtggc agtcggcaag ctggccgacg
    18121 cgtcgcgatt ccagcgcggc ggtccgggtt acgggcagaa cgtgagcgca gcggaactca
    18181 agcgaacgct gtcgctcgcg ccgtgacgtt cctgcactgg cgaagcttcg ccttgccggt
    18241 cacgccggga gagcgcgcgg cggcgctgtg gtcgttcgcg tatttcttca cgctgctcgc
    18301 cggctattac gtgctgcggc cgctgcgcga ccagatgggc atcgcgggcg gcgtgaaggc
    18361 gctgccgtgg ctcttcacgg cgaccttcgt cgcgctgctc gtcgcgcaac cgctgtacgg
    18421 tgcgctggtc gcccgcctgc cgcgcacgcg attcattccg atcgtctatc acttcttcgc
    18481 gctgaacctg gcgattttct ggctgttgct gacactcggc atctccacca cgctggtcgc
    18541 gcgggtgttc ttcgtctggg tgagcgtgtt caaccttttc gccgtcgcgg tgttctggtc
    18601 gttcatggcc gaccttttca cgagcgagca ggggaaacgg ctgttcggct tcatcggcgc
    18661 cggcggtacg gcgggtgcac tgctcggccc ggtgatcacg atcggactct cggtcccgct
    18721 cggcccggcg aacctcctcg tcgtggcgat cgtgttcctc gagctcgcgg tgctctgcgc
    18781 gcatcgtctc gagcgcacga ccgcgtcgca tccagcgtcg cccgttcgcg agcggcggct
    18841 tggcggcagc gcattcgccg cgctgccggc gctgttccgc tcaccctatc tgcttggcgt
    18901 aggcgcgtgg gtgagcctgc tctcgtttgg cgcaaccatc ctgtatttcg agcaggccaa
    18961 catcgtctcg gcgacggtgc acggggccgg tgcgcagacg cggatcttcg cgtcgatcga
    19021 tctcgccgtc ggcttgctca cgctcgcgac gcaggtgttc gcgacgggtc gcgtgctcga
    19081 gcgcttcggc gtgggcgcgg ccgcggccgc gctgcccgcc gtgtacgtcg ccggcttcgc
    19141 cgtcctggcg gtggtgccca cccttgccgt cgtgctcgtt ttccaggtcg tccagcgctg
    19201 gatgaacttc gccatcgcca atccggcacg gcaggtgttc ttcaccgtgg tggaccgaga
    19261 ggacaagtac aaggcgaaga atctcatcga cgtcgtgatc taccgcggct ccgacgcgct
    19321 gtacggctgg gtgttcgaca gcctgcagat gatcggactc aagctcgcgg gcattgccct
    19381 ctggtcgctg ccggtcgtgg ccggatggct cgtgctgtcg gttgcgctgg ggcgaacgca
    19441 ggaacggcgc gccgcgcagg acatcgccgt tgcgcagggg gattgacgat gatcggtctc
    19501 acccgccgcg agttcgttgc gctcgtcgca tcatcggtgt tcacatcgcg cgtcgtcggt
    19561 gagacgagca attcgccgat gattgcgcgt cccattcccc attcgggtga acgactgccg
    19621 gcggtgggac tcggcaccgc aagcgtgttc gaccgcgatg ccgatgacac gcgccgttcg
    19681 gccgcacaag tcgtgcgtgc gctgatcgat ggcggcgcgc gtctcatcga taccgcatcg
    19741 acttatggcg atgccgagct cgtgctcggc aaggtcatta cggccggcag cgtgcgcgac
    19801 aagctcttta tcgcgacgaa actcgaggag cccgacgaat ccgagctcga gcgctcgctc
    19861 acgcgcctgc ggacacggaa ggtcgacctc ctgcaattgc acaacgtgcg cgacccccgc
    19921 cagtcgttgg cgcgattccg ggcatggaag gcgcagggca tctgccgcta cgtcggcatc
    19981 acgtcgacgt atcacagcga tttcgacgcc gtgttcgcgg tgctcggccg tgagaagccc
    20041 gatttcgtgc agatcgacta ttcgatcgac aaccgtgtgg tggaagcgcg aatcctgccg
    20101 ctcgccgcgg acgtcggtgc ggcggtgctc actgcgctgc cgttcggacg tggccggctg
    20161 ttccgcgcgg tgcgcgacaa gccgatcccc gactgggcgc ggccgttcgc cgggagctgg
    20221 gccgtgttct tcctcaaata cctgctcgcc gacgagcggg tgacggcggt gattccaggc
    20281 accaccagcg ccgcgcacat ggccgacaat ctcggcgcca tgcacggcgc gctgcccgac
    20341 tcggcgcagc gcaagcggat ggtgtcgttc atcgcgggtc tgtagcgcga gtcagataag
    20401 tttcgccgcc atcggccgca ttcgcgttac cggcgcttcg ggattgcggg agacgaaggc
    20461 gatttgccgc cgctcgaaaa gccgcaggag ttcacagcag cggtcgtcgg ctttatgcgg
    20521 gcgaatgctc ggtgacggcg accacgcgct cgacgacgcg gtcaatcgcg tcgaatggcg
    20581 acgcgggttt aagtgcctct tcgaacgcgg cgtacgtttc gaagtcggac gcctgcaacc
    20641 actgaaacga cttcttcggg ccctgatacc acattccccg cagggcgaag taatcctcca
    20701 gcagggcggt gagcagccag acccggcgaa aattaccttc gatgtcggaa cgccgcatgc
    20761 gtaatcccat ctttcgcgcc caggagcggc gtgcttcgat ttcgtctgcg ggaaggggtt
    20821 cgggaccggt acggaaaatg acgtcgagag cagccagaaa gtgggtcgcc tcgacattac
    20881 gctggctgag gattctgctt ccgcggaggg taaggtgctc cttcgtgggc ttgcgaagta
    20941 ccgcttccgg atacacgaac acgtccagga atttcccgtc gaccacacga gtgtctcgct
    21001 tcgacacggg aacgtcagcg aacgcggcga tatcgtagtc gctgaactcg ttctcggtac
    21061 cgtcggcgcg cgagccatac agcaggatgg tgtgagcacc gaagttgagg acgagctcgg
    21121 tgcagaggtg gtgcagcaac gtatccacga tgctccgtta tccgcgtgcc cgttgatact
    21181 ggccggtcgt gggcgcttga ttctcggaca agtgcacgct gctactccag atgctcgatg
    21241 tcccggggca cgccaaccca ggaatggatg cgcgcttcgt agaccgagcg ggtcggcgac
    21301 gggaattgtg gatcggcaaa ggcgccgaca ggaatcgcgt agacgtcctc ctgaccttcg
    21361 agcatgtagt acacggtgga cccgcaatca gggcagaagt gaaacgtcgc ttccttgccg
    21421 gaatctccga tccggacgaa cgccttcgat ttgccgcttg tcgttacgct tcgccgcggg
    21481 aagcgagcct gtgcaccgaa gacgctgccg gtgcgccgct ggcaggcaag gcaatgacag
    21541 atggagacgc ggatcgggtc ttcagcagcc accgcggtga gctgcccgca gctgcatgat
    21601 gcggttcgcg cgatcatcgc gtcactgaca cgaagctagc ccggcgtcgc aatcttgagc
    21661 ccgacgatgc ccgcgatgat gagaccgaga ctgacgattc ggccggcatt cgcaacttcg
    21721 ccgagcagca cgatacccag gacggcagtc cccaccgcac cgacgccgac ccagaccgcg
    21781 taggaggttc ccacggggag ggacttcatg gcgatgccga gcaatccgag gctgatgatc
    21841 atcgccagaa cggttccgat cgtgggccac agccgcgtga agccgtctgt gtatttcaag
    21901 ccaatcgccc agcccacttc gaacaggcca gcgagcacga ggagcaacca ggccataggg
    21961 tctcaccctc cctatacgat ggacccgacc aagggtagtg gcgccctgac ttggcgccga
    22021 tagacggcgt acggttgtcc gaaggagcga acgagcatct cgtcttcaac ggcgattcga
    22081 tagctgtacg ccgctgatgt cgctgcgagc gaagccgagc acgggcaccg cagcagacag
    22141 cgagacggcc aacgtgctgt tgcgatccga ggggcatagg gatcaggcac cgaacacctc
    22201 acgcagaaga ccagcgagat gctcggcctc ggccgcagga agcggcttgg cgcgggacgg
    22261 attgagctcg tcaccggcat aaacagggac gaggtgaata tgaacgtgcg ggacggcgaa
    22321 cccttcaacg gcgatcgcga ttcgcttggc cgatgtgacc gatcgtagac caggcgccaa
    22381 ttgggccgct gcagcccaca gtgcttgata tcgcggcggc tccatgtcga atagatagtc
    22441 aatatgcgat ttcggaatca gcaccacatg gccagggttg atgtgttcaa gcggaaagaa
    22501 agcaaggtga tcgttatcct caaaaacgat cgttgccgga acatggcgag caacgatttc
    22561 acagaagatg cagcgagtga cgtttgccat ggtgcggtgc ctaaagtaac aaagttagcc
    22621 ggccggcaaa cgacacgcgg accgaagagc aaagcgtgtg gctgcggatc cggctaaccg
    22681 caaggtcagg ccgcagcaat gcaacgtagc gctcacgaaa gctgcatacg aacaattttt
    22741 atggaaagtg gcgaccttcc gcggagtatc tcgctttcgc cgaccttcgt gaagccgtac
    22801 ctctcatgga acgcttgtgc gttcaatagg gcgagagccg tgatggcgca cggcgcgagc
    22861 gcgcgtgctt tggccggcga tccagtgaac gggagccggg caaagtcggt cgatagatcg
    22921 agcagggcgc gtgcccgtcg acggtaaggt caggccgcac atacgccctc taccaactcg
    22981 tggggaaatt caaccaggaa ttccggtgca gcagcgcgca attcctcaag ggatccatgg
    23041 ccccagagca cgccggcagc ggcgagtccg ttcgccctcg cggcaaggat atcaacggcg
    23101 cgatcaccga ccatgatgaa agacccgtcg atcgattcgg ttgcaaggag ggcgccgagt
    23161 tgttctctct tcgagattcc gatgtcgcca ccgctgatga agcggaagtg ttcccggatg
    23221 ccgaagagcc ttaggatacg atcggcgaag tcggcgcgtt ttgaggtgca aagtccaagc
    23281 acggcgcccg ttcggaccaa agcgcgcagc gcttcgagaa tgccagggta aataacattc
    23341 tcggaaaacc cgatcgcagc gtagcgctcg cggtacttgg aaaccagcgc ggcaatgcgc
    23401 tgaggtgtcg catcatcgac gatcgaccgg aatgtttcat caagcggtgg accaatgaat
    23461 cgtgacaccg cacgctccga gatgagcgga tagccaaaat actgaagcgc gtaggtgagt
    23521 gaacgaccga taccgacggc gggatcgctg atggtcccgt caagatcgaa gacgtacgaa
    23581 gcggctcgga gcatgcggcc taactacagc accttgcgca tgagaatacc aacaccaagc
    23641 cgcgtcattc gctttcccag tatctcaaat ccacgggcca tgtagaaccc ttcagcatgc
    23701 gggttaccga ccacatgaat tgcttttgca ccgccggtac gcgcttcctg ggcgcaacga
    23761 tcgagcaacc tgctgccgaa gcctcgacgc cactcggttg gttcaacgaa tagtgcatcc
    23821 agctcgaggt tgccatcatc gcgacgaaga atggcggcaa atcccttggt cgacctcgcg
    23881 acctcaacaa caaacaccct gccgcgggcg atctgctcgc ttgaaagcgc aatggcatct
    23941 ggatttgcga gaagagcctc gcggtctccg ggattgttca acgaagcgcg caactgcagc
    24001 gcttcgagcg cgttcctctc ggatgggcgc gctgttctga caagaacctc gttctccatt
    24061 cgtgccttcg catgtgccgg ggccagaaat ggcccccggt tggacattat tcttggtttc
    24121 ttgagtggat cgctcggcgg gttaagccgc ttgtcgctgc ggctggctca caagcgcgca
    24181 gttcagttga gcttggcctg aaggaatctg acgacttcct ggttgaacga cgcatggaac
    24241 agcttgcgat cgaagccttt ggcatcgcgg cacagcaacg gcgggccgat cagaccacac
    24301 gggacgagaa atgaaaaatg gccggcgccg ggaaccacgc gcagatccgc cctggcgccc
    24361 aatgcggcct gtacgggtgc tgcgttgctg gcggtcggaa cgttgttatc catggctccg
    24421 ctccagatct gaaccgggac cgtgaccttc gcaagtccgt tcggaacgaa cgtgaatccg
    24481 taacccggcg cggcgatcac tgccgccttc actcggggat catgtgaaaa ggactgcgcc
    24541 atgggaacgt cgtcgcctac cggtttccac aatcgacaca cgaattctgg cgttgttgcg
    24601 caatggccgg caatcagcgc caggtccgga gtacccccga ttgtcgtcaa ggcggtgaag
    24661 gcgccggcag agaacccgaa catgccgatc ttcgacgggt cgatgcgcgc gtggtcgggc
    24721 catgtcgtca ggagaaaatc gatgacgacg ggcacgtgcc gcgtccgtgc gacgagccaa
    24781 tccggcgtgc cgacggcgct ctggtcggca tagttgtcac ccgtgtgcat cggcgcggcg
    24841 acaacgaagc cttccgatgc gagcgccagc gcggtatccg cgtgactgcc cgggccgccg
    24901 ccgctgccat gcgaaataat gacgaggcgc agggaggttc cggcgaccgg tgcatcgttg
    24961 gcgacaaatt gcacggtgag tcccagaagc gccggccgtg ctcccgcttc cgtcggatac
    25021 cagacggcca cgctcaaggg cggcccgctc ctgtcgggga catgcacgac ctggaaaccc
    25081 acgggacggg acgggcgcaa acccgtcgcc acctggaacg ccataactcc gacgacggcg
    25141 actgcaagca gcgcgatcgc gccgaatatc catttcttca tctcggtggt ctcgacagcg
    25201 gttttcgatc cggcagtttc aagtggcatt gaaccattga cgtactgtga aatgcggtat
    25261 agccggatac tcgagacagc ctcgaatctc gccttttcag tcgtccgatt ggcgcgcctc
    25321 gagcggaaga tcgagcacgg ccactatcgt atcgtcgccg cggcggcgca agttcagttt
    25381 cgcgtcgccg ccgtaaagcg tctccaggcg ctcgcgaaag gccaaggccg acagcgtgcc
    25441 tgcaaacctc gagggcctgc tgcaatcgct gctgaaagcg ggcgcaccca gggagtacct
    25501 gcgctggatc aggagacgct ggccgtcagc gatcccgacg cgcatttgtt caagtcgatg
    25561 tagccgcgct tccgccgaca ggtcgagatt cctggacacg ccgttgcaag tgacaactag
    25621 ccgacctgcc cgtggagcgg agacgcgacg ggctacccgt tgagaaaata gatgtccggc
    25681 ttgtcaacga cgcctacgtt ctgcatcgtt tgtcgcaaat ccgcagactc ggcaaatgcc
    25741 tttgcccgcc tgagatcctc tgcctgaaaa aggagaacga cctcattggc atcgctcgcg
    25801 ccccggagaa gatgcttctc ggtgaggccc gccgcggcgc gctttggcaa atgcgcgtcg
    25861 tacccgcgct tccattgcgc aaagtctcta accttgtgtc gtatgagtaa ataactggcc
    25921 attgcaaa
//
LOCUS       DASSVX010000171         3691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_340181, whole genome shotgun sequence.
ACCESSION   DASSVX010000171 DASSVX010000000
VERSION     DASSVX010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340181"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VFO53_07335"
     CDS             <1..787
                     /locus_tag="VFO53_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET8876125.1"
                     /translation="IVENKPGAGGNIGADYVAKSRADGYTVVMGALSTHAVNPSLYPK
                     MPYDAKKDFAPITLVAVTPNVLVVNPELPVHSVQELIGYAKAHPGKLSFGSGSVGSAG
                     HLAGELFKVDAGVDMVHVPYKGAAPAMQALLAGDTQLMFDNLASAMSQVKAGKLRALA
                     VTTAQRSNLAPGLPTMAEAGLSGFDISTWFGLLAPAGTPPAIVDKWNADVTKILSAPE
                     MRGRLASQGAEAAPGTPAEFARFIDSELVKYARIVKASGAKVD"
     gene            complement(918..1298)
                     /locus_tag="VFO53_07340"
     CDS             complement(918..1298)
                     /locus_tag="VFO53_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HET8876126.1"
                     /translation="MSARLAIVAASILMAVAVALGAFGAHALKARVAPEMLAVWQTGV
                     TYHAWHAIGLLLLGILMLHAPGSAAFRVAAWLFIAGIALFAGSLYLLALGAPRGVGAI
                     TPFGGVAFIAAWVAVAVGVWSWRG"
     gene            1344..1709
                     /locus_tag="VFO53_07345"
     CDS             1344..1709
                     /locus_tag="VFO53_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET8876127.1"
                     /translation="MTIERRNVGKRLSDLVIYAPPPSGRLVYLAGQVAEEPKADITLQ
                     TRSVLAQIDRLLDEAGTDKTKILSATIYLADMSDYQAMNAVWDHWVPPGETPARATVE
                     AKLAHPDYRVEMQIVAAAG"
     gene            1811..3022
                     /locus_tag="VFO53_07350"
     CDS             1811..3022
                     /locus_tag="VFO53_07350"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012315598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinyldiaminopimelate
                     transaminase"
                     /protein_id="HET8876128.1"
                     /translation="MPVTRALFDDVMVPCFAPAPFVPVRGEGSRVWDQDGRMYIDFAA
                     GVAVTSLGHCHPAMIRALDAQARTLWHISNWFTNEPALRLAKRLTDATFAERVFFCNS
                     GAEANEAALKLARRYAHDRSGPARTRVVSTLNAFHGRTLFTVTAGGQAKYASGFGPNP
                     SGFTHLRYNDIAALEAEFGAHGNEICAVILEPMQGEGGMTPGTPDYLQAARRLCSAHG
                     ALLILDEIQSGMGRTGALFSYMQKGIVPDILTSAKGLGGGFPIGAMLTTADIASAFSV
                     GVHGTTYGGNPLACAVAGAVFDVINTTEVLDGVKARHALFMEGLRAINARRPVFRDLR
                     GEGVWIGCELGDAWRGKAADVVKAAGGAGLLVLMAGPDVVRIAPSLVISLDEILEGLA
                     RLETAVNRALV"
     gene            3033..>3691
                     /locus_tag="VFO53_07355"
     CDS             3033..>3691
                     /locus_tag="VFO53_07355"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020329790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HET8876129.1"
                     /translation="MFVIRPIARDDLPAVLALSERTGSGLTTLPANRERLLERIERSL
                     ASFDKVAARQDACYMFVLVEANATGQRVVGISAIEAAVGLSEPWYNYHVGTLVHASRT
                     LGVYTVTPTLFLSNDHTGHSELCSLFLDERYRVGRNGPLLAKSRLLFIAEFSELFATK
                     IFAELRGRLEADGTSPFWEGLGRHFFAMEYSAADYLTGLGQKSFIAELMPKHPVYVNL
                     LP"
BASE COUNT          625 a         1257 c         1238 g          571 t
ORIGIN      
        1 gatcgtcgag aacaagccgg gcgcgggcgg caacatcggc gccgactacg tcgccaagtc
       61 gcgcgccgac ggctacacgg tggtgatggg tgcgctgtcg acgcacgcgg tcaacccgag
      121 cctctatccg aagatgcctt acgacgcgaa gaaggatttc gcgccgatca cgctcgtcgc
      181 ggtcacgccg aacgtgctgg tcgtcaaccc cgaactgccg gtgcactcgg tgcaggaatt
      241 gatcggctac gcgaaggcgc atccgggcaa gctgtcgttc gggtcgggca gcgtcggcag
      301 cgccggtcat ctcgccggcg agctcttcaa ggtcgacgcc ggcgtcgaca tggtgcacgt
      361 gccgtacaag ggcgccgcgc cggcgatgca ggcgctgctc gccggcgaca cgcagctcat
      421 gttcgacaac ctcgcgagcg cgatgagcca ggtgaaggcg ggcaagctgc gcgcgctggc
      481 ggtcaccacc gcacaacgct cgaacctcgc gcccggtctg ccgacgatgg ccgaagcagg
      541 cctttccggc ttcgacatct cgacgtggtt cggcctgctt gcgccggccg ggacgccgcc
      601 ggcgatcgtc gataaatgga atgccgacgt gacgaagatt ctgtcggcgc ccgagatgcg
      661 cgggcgcctc gcgtcgcagg gcgcggaagc ggcgcccggt acgccggccg agttcgcgcg
      721 gttcatcgac agcgagctcg tcaagtacgc gcggatcgtg aaggcgtcgg gcgcgaaagt
      781 ggattagccg cgataaacgc gagccacgca ccgcttcgcg gagataggag atcggggtca
      841 gagctgtaat tattcgaatt cgcgaataat tacagctctg accccgatct tcgaattcgc
      901 gcggtggatt cgctgcgtca gcctcgccag ctccatacgc cgaccgcaac cgcaacccac
      961 gccgcgatga acgcgacgcc gccgaacggc gtgatcgcac ccacgccgcg cggcgcgccg
     1021 agtgccagca gatagaggct tcccgcgaaa agcgcgatgc cggcgatgaa cagccaggcg
     1081 gccacgcgaa aggccgcgct cccgggcgca tgcagcatca ggatgccgag cagaagcagc
     1141 ccgatcgcat gccatgcgtg atacgtgacg ccggtctgcc acaccgcgag catctcgggt
     1201 gccacgcgcg ccttcagcgc atgcgcgccg aacgccccga gcgcgacggc gacggccatc
     1261 aggatcgatg ccgcgacgat cgcaagtcga gcgctcattt tcgactaaac ttacggggcg
     1321 aagccacgtc ctacgataac accatgacca tcgaacgccg caatgttggc aagcgccttt
     1381 ccgacctcgt gatctacgcg ccaccgccga gcggccggct cgtctacctc gccgggcagg
     1441 tggccgagga gccgaaggcc gacatcacgc tgcagacgcg ctcggtgctc gcgcagatcg
     1501 accgcctgct tgacgaggcc ggcaccgaca agacgaaaat cctttcggcg acgatctacc
     1561 tcgccgacat gagcgactat caggcgatga acgccgtgtg ggaccactgg gtgccgccgg
     1621 gcgaaacgcc cgcgcgcgcc acggtcgaag cgaagctcgc gcatcccgac tatcgcgtcg
     1681 agatgcagat cgtcgctgcg gccggctgac gcgaacggac atcgccatgc ctctgtcccc
     1741 cggcgcgcga gcgctcgtcg ccgagaccgc gcgccgcgac gcgaccaccc cgcatcccga
     1801 agcggcgacg atgcccgtca cgcgcgcgct gttcgacgac gtgatggtgc cgtgcttcgc
     1861 gcccgcgccg ttcgttccgg tgcgcggcga aggctcgcgc gtgtgggacc aggacgggcg
     1921 gatgtacatc gatttcgccg cgggcgtcgc ggtgacgtcg ctcggccatt gccatccggc
     1981 gatgatccgc gcgctcgatg cgcaggcgcg cacgctgtgg cacatctcga actggtttac
     2041 caacgagccg gcgttgcggc ttgcgaagcg cctcaccgat gcgacgttcg ccgagcgcgt
     2101 gttcttctgc aattcgggcg ccgaggcgaa cgaggccgcg ctgaagctcg cacggcgcta
     2161 tgcgcacgat cgctccggtc ccgcgcgcac ccgcgtcgtg tccacgctga acgcgttcca
     2221 cggccgcacg ctgttcacgg tcacggccgg cggacaggcg aaatacgcga gcggcttcgg
     2281 gccgaacccc agcggcttca cccacctgcg ctacaacgac atcgccgcgc tcgaggccga
     2341 gtttggcgcg cacggcaacg agatctgcgc ggtgatcctc gagccgatgc agggcgaagg
     2401 cgggatgaca cccggcacgc ccgactacct gcaggcggcg cggcggctgt gcagcgcgca
     2461 cggtgcactg ctgatcctcg acgagatcca gagcggcatg ggccgcaccg gggcgctgtt
     2521 ctcgtacatg cagaagggca tcgttcccga catcctgacc agcgcgaagg gtctcggcgg
     2581 cggctttccg atcggcgcca tgctcaccac ggccgacatc gcgagtgcgt tttcggtcgg
     2641 cgtgcacggc acgacgtatg gcggcaatcc gctcgcctgc gcggtggctg gcgccgtgtt
     2701 cgacgtcatc aacaccaccg aagtgctcga cggcgtcaag gcgcgtcatg cgctgttcat
     2761 ggaagggttg cgcgcgatca acgcgcgtcg ccccgtgttc cgcgatctgc gcggcgaagg
     2821 cgtgtggatc ggctgcgagc tcggcgacgc gtggcgcggc aaggctgccg acgtcgtgaa
     2881 ggccgcgggc ggcgcgggat tgctggtgtt gatggcgggc cccgacgtcg tgcgcatcgc
     2941 gccgtcgctc gtcatttcac tcgacgagat cctcgagggg ctcgcacggc tcgagaccgc
     3001 ggtgaaccgc gcgctggtct aactggggca cgatgttcgt catccggccg atcgcgcgcg
     3061 atgatctgcc ggcggtgctc gcgttgtccg agcgcaccgg cagcggcctg accaccctcc
     3121 ccgcgaaccg cgagcgtctg ctcgagcgca tcgagcgctc gctcgcatcg ttcgacaagg
     3181 tggccgcgag gcaggacgcg tgctacatgt tcgtgctcgt ggaagcgaac gcgaccggac
     3241 agcgcgtcgt gggcatcagc gcgatcgaag ccgcggtcgg gttgagcgag ccctggtaca
     3301 actaccacgt cggcacgctc gtccatgcgt cgcgcacgct cggcgtctac acggtgacgc
     3361 cgacgctgtt cctgtcgaac gaccataccg ggcatagcga gctctgctcg ctgttcctcg
     3421 acgagcgcta tcgcgtcggc aggaacggtc cgttgctcgc gaaaagccgc ctgcttttca
     3481 tcgccgaatt cagcgagctt ttcgccacga agatcttcgc cgaactgcgc gggcgcctcg
     3541 aggccgacgg cacgagcccg ttctgggaag gactgggacg ccactttttc gcgatggaat
     3601 attcggcggc ggactacctg accggtctcg gacagaaatc gttcattgcc gaactgatgc
     3661 cgaaacaccc ggtctacgtg aacctgctgc c
//
LOCUS       DASSVX010000172        12575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_856770, whole genome shotgun sequence.
ACCESSION   DASSVX010000172 DASSVX010000000
VERSION     DASSVX010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856770"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /gene="mfd"
                     /locus_tag="VFO53_07360"
     CDS             <1..1838
                     /gene="mfd"
                     /locus_tag="VFO53_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET8876130.1"
                     /translation="YSGASPDAAPLHELGSGQWEKAKRKAAQQAHDTAAELLNLYAQR
                     AARNGHAFSFKPHDLEAFSEGFGFEETPDQQAAIDAVVADLAAGRPMDRLVCGDVGFG
                     KTEVALRAAFIALTDGKQVALLVPTTLLAEQHFQVFSDRFADWPVKLAELSRFRSTKE
                     VKAVLEGLASGRVDLVIGTHKLIQPDVKFRNLGLVIIDEEHRFGVRQKERLKRLRAEV
                     DVLTLTATPIPRTLAMSLEGIRDFSVIATAPQRRLAIKTFVSPFSMGIVREAALRELK
                     RGGQIYFLHNDIDTIRTTAERLAQLLPEARIAVAHGQMGERELEHVMREFYAQRVNLL
                     VCSTIIETGIDVPTANTIIIDRADRFGLAQLHQLRGRVGRSHHQAYAYLLTPPHEALS
                     AQAKKRLEAIQMMEDLGSGFFLAMHDLEIRGAGEVLGDEQSGEMQQVGFQLYADMLKA
                     AVSALKAGREPDLSQPLGVTTEINLHAPARLPELYCNDIHERLVLYKRLASCESIDEL
                     ELLREELVDRFGPAPEPAQALIACHRLRLLARPLGVTKIDAGPERSTLSFVKSPPFDA
                     GKLILLVQKDGRIRFAGPDRLRIERAAPALSDRVTLVREFLAKLQ"
     gene            1903..2829
                     /locus_tag="VFO53_07365"
     CDS             1903..2829
                     /locus_tag="VFO53_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8876131.1"
                     /translation="MKAVADAMPPQMHVFVRDWLSANQVVLRGPDGGVVIDSGYGKHA
                     PLTLALVASRMGLDGAPLAKLVNTHCHSDHMGGNAALLRAYGCRIAVPAGEAPLIASW
                     DQRALLLDYAGQRAERFEVDEVLQAGSTHVWGELEWRALAAPGHDMGALVFFNDEHAI
                     LVSGDALWQSGYGFVMPPDIDAEALPATRATLGMIAGLGVRWVIPGHGEPFGDIDAAL
                     ERAFRRTEAFEADPLRLARHAVKVVLVFALLDRERMLLADMPDYVERVEMFRDFNQRY
                     FKVPPAELAAQLVDELERVGAVRREDGYLLPR"
     gene            complement(2872..3456)
                     /locus_tag="VFO53_07370"
     CDS             complement(2872..3456)
                     /locus_tag="VFO53_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET8876132.1"
                     /translation="MNSRFAVAAVALCTALPVTAAETYTLDPVHSAPSFETGHLGFSH
                     QVGAFMKSSGKVTIDRAAKTGTVDVTIDSASVRTHDSTRLDAIVKGEKYFNAEKYPTI
                     TFKSSSVRFDGDRVVGVDGELTMLGVTKPVALTVVDFRCGAHPFNKKPMCGGEATAMI
                     KRSDWGMTAGLPFAPADEVKLRIPIEAYQDQPQS"
     gene            complement(3572..4135)
                     /locus_tag="VFO53_07375"
     CDS             complement(3572..4135)
                     /locus_tag="VFO53_07375"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET8876133.1"
                     /translation="MIVRRIAAGASLALVLALPVAYGQGVVAAKSEIAFTVKQMGVKF
                     DGRFTKWTADVVFQPKTLATSKAVLDVDLGSIDLASGESEAEAKGPLWFDTAKFPVAH
                     FVSTAIRDVGADRYEVAGKLTLKGVTRDCAVPITVATDASGNRVAQGAFSIRRLDYRI
                     GEGEWADTGIVDNDIVVRVRMMLAPAT"
     gene            complement(4132..4683)
                     /locus_tag="VFO53_07380"
     CDS             complement(4132..4683)
                     /locus_tag="VFO53_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HET8876134.1"
                     /translation="MSETYNRGAIALHWITAALIVANLLLGLSMVALPISPRKLHWYL
                     FHKSIGVTVFLLTSLRLAWRALRPHPAPVPMPSWQRRSAAVSHALLYLLLFVIPLSGW
                     VYSSATGVQVVYAGLVPLPDLVPRDRALADVLRLVHVSLNALFFVVICVHVAAAVKHH
                     LIDRDAALARMLPLVKPNAAVRS"
     gene            complement(4782..5393)
                     /locus_tag="VFO53_07385"
     CDS             complement(4782..5393)
                     /locus_tag="VFO53_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HET8876135.1"
                     /translation="MPIAEIFSAGVLLLLVIDPFGNVPIVVSALANVAPARRVRVVLR
                     ECFAAYLILVAFMFGGQAFLRWLQLSEVSLAIAGGIILFLIALRMVFRHPEGVFGDPP
                     GDEPFLVPLAIPSIAGPSALATVMLMVSRDPTHRLAWLIALTAAMIVATIVLLAAHWL
                     QRVLGERGMIAVERLMGLVLTALAVQMLLDGVRNFVATLAQGG"
     gene            complement(5409..7151)
                     /gene="ggt"
                     /locus_tag="VFO53_07390"
     CDS             complement(5409..7151)
                     /gene="ggt"
                     /locus_tag="VFO53_07390"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET8876136.1"
                     /translation="MSFARAVACIGGACTALAFAQPLPPEQPSGWIDKRPVTSQRFMV
                     AAAHPLATRAGYDVLRAGGNAVDAAIAVQLVLGLVEPQSSGLGGGAFLLYHDARRRRL
                     VAYDGRETAPAAARPERFLDADGRPLAFLDAVASGLSVGVPGTVRLLETVHRRHGKLP
                     WPRLFQRAIALAETGFAVSPRLHGEIAAQARWPQRRARDYFLTPEGAPPAVGSVLRNP
                     AYARTLRTLASQGAKAFYDGPIADDIVRTVDRAPRHPGDLTRADLAHYRARVRTPVCD
                     GYRGYRVCGMPLPSSGGITVLQTLKMLEPYDIASMGAASFWSVHFASEAERLAFADRD
                     VYLADPDFVPPPAGLLDDGYLHARSLLISTRGSLGVAAPGDPAHHLAVASATAWSTGV
                     TADFPSTSQISIVDADGNAVSMTTTIEYGFGSRLMTEGGFLLNNELTDFSFVPVDNGK
                     PVANRVEPGKRPRSSMAPTIVYDKAGAVAIVTGSPGSSAIIDYVVKTLLAIIDWGLDP
                     QAAAALPNFGSRNGPTELEKDTPVAALAPKLAALGHDVRVIEETSGVQTIVRARGGLV
                     GGADPRREGTVLGR"
     gene            complement(7154..7429)
                     /locus_tag="VFO53_07395"
     CDS             complement(7154..7429)
                     /locus_tag="VFO53_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876137.1"
                     /translation="MARCECCGNDYDKSFTVTMAGVAHTFDSFECAIYTLAPACEHCG
                     CRIIGHGMEVSGRYFCCAHCAHEEGAMSLKDRAQIGDRDPVPAPPDR"
     gene            complement(7449..9593)
                     /locus_tag="VFO53_07400"
     CDS             complement(7449..9593)
                     /locus_tag="VFO53_07400"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET8876138.1"
                     /translation="MSNSLIFALVCAIAAIVYGAVSIRWVLAKPAGNPRMQEIAAAIQ
                     AGAKAYLNRQYTTIGIVGVILFILIGIFLSWRTGGGFAVGAILSGLAGYIGMNVSVRS
                     NVRTAEAARTGLGEALAVAFRGGAITGMLVVGLGLLGVAGFYWFLVASAHYAPNFENE
                     GLHAAIEPLVGLAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAV
                     IADNVGDNVGDCAGMAADLFETYAVTIVATMLLGALLLPTMATAAVTYPLVLGGFSIL
                     ASIVGCYFVKARSGGKIMNALYRGLIVAGVISAIGFYFITQRMMGDIGSANAALSPLR
                     IWGASLVGLVLTAAMVVITEYYTGTEFKPVKHVAAASTTGHATNIIAGLGVSMKSTAW
                     PVIAVCIAILASFLCAGLYGIAIAATAMLSMAGIIVALDAYGPITDNAGGIAEMADLP
                     ASVRAITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHALDTAGLSTSFDLSNH
                     MVIIGLFLGGLIPYLFGAMAMEAVGRAAGAVVVEVRRQFRDIKGIMEGTAKPEYGVAV
                     DMLTRAAIREMIVPSLLPVLVPIVVGLVLGPQALGGLLMGTIVTGLFVAISMTTGGGA
                     WDNAKKYIEDGNYGGKGSDAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIVALMIVP
                     LLALHGPGHVVGTTAAVTAPVSAPAPAAAPAK"
     gene            complement(9844..11109)
                     /locus_tag="VFO53_07405"
     CDS             complement(9844..11109)
                     /locus_tag="VFO53_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET8876139.1"
                     /translation="MSSRRNAFYAQSGGVTAVINASAAGVIETARKHRARIGRVYAGR
                     NGIIGALTEDLIDTSREPARAIAALRHTPGGAFGSCRYKLRSLDENRAQYERLIDVFR
                     AHDIGYFFYNGGNDSADTCLKVSQIARALDYPLVAVHVPKTVDNDLAVTDNCPGFGSV
                     AKYVATSMREAAFDVASMAKTSTKVFVLEVMGRHAGWITAAVGLADDEATPIPLVLLF
                     PEIAFDEAAFLAAVDARVKTFGYCCVGVSEGLRNAQGELMGEAGTKDAFGHAQLGGVG
                     PQIAALVKDRLRYKYHWAVADYLQRAARHLASKTDLDQSYAVGKAAVELALRGQNAVM
                     PAIVRVSDRPYRWKLGVAPLADVANREKMLPRNFITRDGYGITERARAYLAPLIRGEA
                     PPPYRDGLPHYVRLKNVAVPKKLAAGFAI"
     gene            complement(11093..12100)
                     /locus_tag="VFO53_07410"
     CDS             complement(11093..12100)
                     /locus_tag="VFO53_07410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8876140.1"
                     /translation="MLPLVSIVVRSMARASLQEALASIGMQDYERIEAVIVAACGAAH
                     PVPPGTIGNHRVRFVPGESARSRPQAANAGLDAATGDWMTFLDDDDVLLPAHVSGLVE
                     ALRNAGTCKVVYTLARARFADGTTQPVGQPFAPIELFERNFIHLSMSLFSRELVSRGC
                     RFDESLDILQDWDFFIQCAQHTHFHFEPRQTFEWRAEAGDSGAGAGVNQDDARFAAFR
                     DRIYAKWAARHDALVERVGGGLQRAADLAQARDLAGAEAVCREVLDASRNDPFALTLL
                     AMVQRAAGRVDDARATQTLACAVRPHDASLARNLALLERTAGAAIADSKPGGSPHVQS
                     T"
     gene            complement(12120..>12575)
                     /locus_tag="VFO53_07415"
     CDS             complement(12120..>12575)
                     /locus_tag="VFO53_07415"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HET8876141.1"
                     /translation="VKERLQQDDCAQGYLLDGFPRTIPQADALKDAGIVLDYVIEIDV
                     PDAAIVERMSGRRIHVASGRSYHVKFNPPKITGRDDVTGEPLIQRADDSEQTVRKRLA
                     IYHTQTEPLVTYYAQWEASGDPKSPRYRKVNGLGTVEGIRDACMAALRS"
BASE COUNT         2105 a         4261 c         4286 g         1923 t
ORIGIN      
        1 gctacagcgg cgcatcgccc gacgccgcac cgctgcacga gctcggcagc gggcagtggg
       61 aaaaagcgaa gcgcaaagcc gcgcagcagg cgcacgacac cgcggccgag ctcttgaacc
      121 tgtatgcgca gcgtgcggcg cgcaacggcc atgcgttctc gttcaagcca cacgatctcg
      181 aggcgttctc cgaaggcttc ggcttcgagg agacgcccga ccagcaggcg gcgatcgacg
      241 ccgtcgtcgc cgacctcgcc gcgggccggc cgatggatcg tctcgtgtgc ggcgacgtcg
      301 gcttcggcaa gaccgaagtg gcgctgcgcg cggcgttcat cgcgctcacc gacggcaagc
      361 aggtggcact gctggtgccg acgacgctgc tcgcggagca gcatttccag gtcttctccg
      421 atcgcttcgc cgattggccg gtgaagctcg cggagctgtc gcgcttccgc tcgacgaagg
      481 aagtgaaggc ggtgctcgaa gggcttgcca gcggccgcgt cgacctcgtc atcggcacgc
      541 acaagctgat ccagcccgac gtcaagttca ggaacctggg gctcgtgatc atcgacgagg
      601 agcatcgatt cggcgtgcgc cagaaggagc ggctgaagcg cctgcgcgcg gaagtcgacg
      661 tgctgacgct caccgcaacg ccgattccac gcacgctcgc gatgtcgctc gagggcatcc
      721 gtgacttctc ggtgatcgcg accgcgccgc agcgacggct cgcgatcaag acgttcgtgt
      781 cgccgttttc gatgggcatc gtgcgcgagg cggcgctgcg cgagctgaag cgcggcggcc
      841 agatctattt cctgcacaac gacatcgaca cgatccgcac gacggcggag cggctcgcgc
      901 agctcctgcc cgaggcgcgc attgccgtgg cgcacggcca gatgggcgag cgggagctcg
      961 agcacgtgat gcgcgagttc tacgcgcagc gcgtgaacct gctcgtatgc tcgacgatca
     1021 tcgagacggg catcgacgtg ccgacggcga acacgatcat catcgatcgc gccgaccgct
     1081 tcggcctggc gcagctgcat cagctgcgcg gccgcgtcgg ccgctcgcat caccaggcgt
     1141 acgcgtacct gctgacgccg ccgcacgagg cactgtcggc gcaggcgaag aagcggctcg
     1201 aggcgatcca gatgatggag gatctcggct cgggcttctt cctcgcgatg cacgatctcg
     1261 agatccgcgg cgccggcgag gtgctcggcg acgagcagtc aggcgaaatg cagcaggtcg
     1321 gcttccagct ctacgccgac atgctgaaag ccgccgtgtc ggcgctgaag gccggccgcg
     1381 agcccgacct gtcgcagccg ctcggcgtga cgaccgaaat caacctgcac gcgccggcgc
     1441 gcctgccgga actctattgc aacgacattc acgagcggct ggtgctctac aagcgcctcg
     1501 cgagctgcga atcgatcgac gagctcgagc tcctgcgcga ggagctcgtc gaccgcttcg
     1561 gtcccgcgcc cgagcccgcg caggcgctga tcgcctgcca tcgcctgcgc ctcctcgccc
     1621 gtccgctcgg cgtgacgaag atcgacgcgg gtcccgagcg ctcgacgctt tcgttcgtga
     1681 agtcgccgcc tttcgacgca ggcaagctga tcctgctcgt gcagaaggac ggccgcatcc
     1741 gcttcgccgg acccgaccgg ctgcgcatcg agcgcgcggc gccggcgctg tcggatcgcg
     1801 tgacgctggt gcgcgagttt ctcgcgaagc tgcagtgaag accggaatgg gggccggact
     1861 ccgcttctcc gggtttgtcc gtgcctggcg cgtgagcaac gcgtgaaagc ggtagccgac
     1921 gccatgccgc cgcagatgca cgtgttcgtg cgcgactggc tgtcggcgaa ccaggtcgtg
     1981 ttgcgcggcc ccgacggtgg cgtcgtcatc gactcggggt atggcaagca tgcgccgttg
     2041 acacttgcac tcgtcgcgtc gcggatgggg ctcgacggtg cgccgctcgc gaagctcgtc
     2101 aacacgcact gccattcgga ccacatgggc ggcaacgcgg cgctgctgcg cgcgtacggc
     2161 tgccgcatcg ccgtgccggc cggcgaagcg ccgctgatcg cgtcctggga ccagcgcgcg
     2221 ctgctcctcg attatgcggg gcaacgggcc gagcgcttcg aggtcgacga agtgctgcag
     2281 gccggctcga cgcatgtgtg gggcgagctc gaatggcgcg cgctcgcggc gcccgggcac
     2341 gacatgggcg cgctcgtgtt cttcaacgac gagcacgcca tcctggtctc gggcgatgcg
     2401 ctgtggcaaa gcggctacgg tttcgtgatg ccgcccgaca tcgacgccga ggcgctgccc
     2461 gcgacgcgcg cgacgctcgg gatgattgcc ggtctcggtg tgcgctgggt gattcccggg
     2521 cacggcgaac cattcggcga catcgacgca gcgctcgagc gcgcctttcg gcgcaccgag
     2581 gcgttcgaag ccgatccgct gcggctcgcg cggcacgcgg tgaaagtggt gctcgtgttc
     2641 gccctgctcg accgcgagcg gatgctgctc gccgacatgc ccgattacgt cgagcgggtc
     2701 gagatgttcc gcgacttcaa ccagcgctac ttcaaggtgc cgccggccga gctcgccgcg
     2761 cagctcgtcg acgagctcga gcgggtgggc gccgtgcggc gcgaggacgg ttacctgctg
     2821 ccgcgctgac tcggggtccg ccgatccggg ccccgacgcc ggcaattccg gtcagctttg
     2881 cggctggtcc tggtacgcct cgatcgggat gcgcagcttc acctcgtcgg ccggcgcgaa
     2941 cggcaggccc gcggtcatgc cccagtcgga gcgcttgatc atcgccgtgg cctcgcctcc
     3001 gcacatcggc ttcttgttga acggatgcgc gccgcagcga aagtcgacga cggtgagcgc
     3061 gaccggcttc gtgacgccga gcatcgtgag ctcgccatcc acgcccacca cacggtcgcc
     3121 gtcgaacctg acgctgctcg acttgaacgt gatcgtcgga tacttctcgg cgttgaagta
     3181 cttctcgccc ttgacgatcg cgtcgagccg cgtcgaatcg tgcgttcgca ccgaggccga
     3241 atcgatcgtc acgtcgacgg tgccggtctt cgcggcgcga tcgatcgtca ccttgcccga
     3301 cgatttcatg aatgcgccaa cctggtgact gaaaccgagg tgaccggtct cgaacgacgg
     3361 cgccgaatgc accgggtcga gcgtgtacgt ttccgcggcc gtgaccggca acgcggtgca
     3421 aagggcaacg gcggcaacgg caaagcggga gttcatggca aggctcctcg tcgatcggtt
     3481 gatgatgcgg gtggaatcgg tcgcgccgcg cgaacgggcg tgaccgcgcg gctattcctc
     3541 gtcctcgcga gggtccccgg cttggcagcg ctcacgtcgc cggcgccagc atcatgcgca
     3601 cgcgcacgac gatgtcgttg tcgacgatac cggtgtcggc ccactcgccc tcgccgatcc
     3661 ggtaatcgag gcggcgaatg gaaaacgcgc cctgcgcgac gcgattgccg cttgcatcgg
     3721 tcgcgacggt gatcggcacc gcgcaatcgc gcgtgacgcc tttcagcgtc agcttgccgg
     3781 ccacctcgta gcggtcggcg ccgacgtcgc ggatggcggt ggacacgaag tgcgcgacgg
     3841 gaaattttgc ggtgtcgaac cagagcggac ccttcgcctc ggcctccgat tcgccgctcg
     3901 cgaggtcgat gctgccgagg tcgacgtcga gcaccgcctt cgacgtggcg agcgtcttcg
     3961 gctggaagac cacgtccgcg gtccacttcg tgaaacggcc gtcgaatttg acgcccatct
     4021 gttttaccgt gaaggcgatc tcgcttttcg ccgcaacgac gccttgaccg tacgcaaccg
     4081 ggagcgcaag cacgagcgcg agcgaagcac ccgcggcgat tcgccgcacg atcatgaccg
     4141 aacggccgcg ttcggcttga cgagcggcag catgcgcgcg agtgctgcgt cgcggtcgat
     4201 cagatggtgc ttgacggccg ccgcgacgtg cacgcagatc acgacgaaaa acagtgcgtt
     4261 gagcgacacg tgaacgaggc gcagcacgtc ggcgagcgcg cgatccctgg gaacgagatc
     4321 cggcagcgga acgagaccgg catagacgac ctgcacgccg gtggccgagc tgtagaccca
     4381 gcccgacagc ggaatgacga agagcagcag gtacagcagc gcgtgcgaaa cagccgccga
     4441 gcgccgttgc catgacggca tcggtaccgg tgccgggtgc ggacgcagcg cgcgccacgc
     4501 aaggcgcagc gaggtgagca ggaacaccgt gacgccgatc gacttgtgga aaagatacca
     4561 atgcagcttc cgcggcgaga tcggcagcgc caccatcgac aggccgagca gcaggttggc
     4621 gacgatcagt gccgcggtga tccagtgcag cgcgatggcg ccgcggttgt acgtttcgct
     4681 caattcgctt ccgcgtcgat tcgaagtcac ggcggcaccg cacgccgccc ttgcgtaccg
     4741 gtaacaggcg ccgcgggcag cgtattccaa tgcgcgatgc gtcagccgcc ctgcgcgagt
     4801 gtcgcaacga aattgcgcac gccgtcgagc agcatctgca cggcgagcgc cgtcagcacg
     4861 aggcccatca ggcgctccac ggcgatcatg ccgcgctcgc caagcacgcg ttgcagccaa
     4921 tgcgccgcca gaagcacgat ggtggcgaca atcatcgcag ccgtcaacgc gatcagccac
     4981 gcgagccgat gcgtcggatc gcgcgagacc atcagcatga ccgtcgccag cgccgagggt
     5041 cccgcgatcg aggggatcgc gagcggcacg agaaacggct cgtcacccgg cgggtcgccg
     5101 aatacgccct ccggatggcg gaacaccatg cgcagcgcga tcaggaacag gatgatgccg
     5161 ccggcgatcg caagcgagac ttcggaaagc tgcagccagc gcaggaatgc ctggccgccg
     5221 aacatgaacg cgacgaggat caggtacgcg gcgaagcatt cgcgcagcac cacgcgaacg
     5281 cgccgcgcgg gcgccacgtt cgcgagcgcg gacacgacga tcggcacgtt gccgaacggg
     5341 tcgatgacga ggagcagcag cacgccggcg gaaaagattt cggcgatcgg catcgcgtcg
     5401 cggccggcct agcggccgag caccgtacct tcgcgccgcg gatcggcgcc gccgaccagg
     5461 ccgccccgcg cgcgcacgat cgtctgcacg ccgctcgtct cctcgatgac gcgcacgtcg
     5521 tgtccgagcg ccgcaagctt cggcgccagc gccgcaaccg gcgtgtcctt ctcgagctcc
     5581 gtcggcccgt tgcggctgcc gaaattcgga agcgccgcgg cggcctgcgg atcgagcccc
     5641 cagtcgatga tcgcgagcaa ggtcttcacg acgtagtcga tgattgcgct gctgcccggc
     5701 gaaccggtga cgatcgcaac cgcgccggcc ttgtcgtaga cgatcgtcgg cgccatcgaa
     5761 gaacgcgggc gcttgcccgg ctccacccgg ttcgccaccg gcttgccatt gtcgaccgga
     5821 acgaacgaga agtcggtgag ttcgttgttg agcaggaatc cgccctcggt catcagccgg
     5881 ctgccgaagc cgtattcgat cgtcgtcgtc atcgacaccg cgttgccgtc ggcatcgacg
     5941 atcgagatct gcgacgtcga cggaaaatcg gccgtgacgc cggtgctcca cgccgttgcg
     6001 gaagcgaccg cgagatggtg cgcgggatcg cccggcgcgg cgacgccgag actgccgcgc
     6061 gtcgagatca gcagcgagcg cgcgtgaaga tacccgtcgt cgagcagccc cgccggcggc
     6121 gggacgaaat cggggtcggc caggtagacg tcgcgatcgg cgaacgcgag acgctcggcc
     6181 tcgcttgcga agtgcacgct ccagaacgac gcggcgccca tcgacgcgat gtcgtacggc
     6241 tcgagcatct tgagcgtctg cagcacggtg attccgcccg acgacggcag cggcatgccg
     6301 cagacgcgat agccgcgata accgtcgcag accggcgtgc gtacccgcgc ccggtagtgc
     6361 gcgagatcgg cgcgcgtcag atcacccggg tgacgaggcg cgcgatcgac ggtgcgcacg
     6421 atgtcgtcgg cgatcggccc gtcgtagaac gccttcgccc cctggctcgc cagcgtgcgc
     6481 agcgtacgcg cgtacgcggg gttgcgaagc accgagccca ccgcaggcgg cgcgccttcc
     6541 ggcgtcagga aatagtcgcg cgcgcgccgt tgcggccagc gcgcttgcgc cgcgatttcg
     6601 ccgtgcagcc ggggcgaaac cgcgaagccg gtttcggcga gcgcgatggc gcgctggaac
     6661 agtcgcggcc atggcagttt cccgtggcgc cgatgcaccg tttcgagcag tcgaacggtt
     6721 ccggggacgc cgaccgacaa cccgcttgcc acggcatcga gaaaggccag cgggcgaccg
     6781 tcggcatcga gaaagcgttc gggccgcgcc gccgccggcg cggtttcacg gccgtcgtag
     6841 gcgacgagcc ggcgccgcct tgcatcgtgg tagagcagga acgcgccgcc gccgaggccc
     6901 gacgactgcg gctcgacgag gccgagcacg agttgcacgg cgatcgcagc gtcgaccgca
     6961 ttgccgccgg cacgcagcac atcgtagccg gcgcgcgtgg cgagcggatg cgcggcggcc
     7021 accatgaacc gctggctcgt gacgggccgc ttgtcgatcc agcccgacgg ctgctcgggt
     7081 ggaagcggct gcgcaaacgc gagcgccgtg cacgcgccgc caatgcacgc gacagcacgc
     7141 gcgaacgaca atgctagcga tccggcggcg cgggcacggg gtcgcgatcg ccgatctgcg
     7201 cgcgatcctt gagcgacatc gcgccttcct cgtgcgcgca atgcgcacag cagaagtaac
     7261 gcccgctcac ttccatgcca tggccgatga tcctgcagcc gcagtgctcg cacgccggtg
     7321 cgagcgtgta gatcgcgcat tcgaacgaat cgaacgtgtg cgcgacgcct gccatcgtta
     7381 ccgtgaagga cttgtcgtag tcgttgccgc agcattcgca tcgcgccatc ggaacctccg
     7441 cttgcaatct acttcgccgg cgccgcggca ggcgcgggcg ccgacacggg cgccgtcact
     7501 gccgcagtgg tgcccaccac gtgaccggga ccgtgcagcg ccagcagcgg cacgatcatc
     7561 agcgcgacga tgttgatgat cttgatcagt ggattgacgg ccggtcccgc ggtgtccttg
     7621 tacgggtcgc cgaccgtgtc gccggtgacg gcggccttgt gcgcatcgga acccttgccg
     7681 ccgtagttgc cgtcctcgat gtacttcttc gcgttgtccc aggcgccgcc gcccgtcgtc
     7741 atcgagatgg ccacgaagag acccgtcacg atcgtaccca tcaagaggcc acccagcgcc
     7801 tgcgggccga gcacgagccc gacgacgatc ggaacgagca ccggcaaaag cgacggcacg
     7861 atcatttcgc gaatggcagc gcgcgtcagc atgtcgacgg cgacgccgta ttcgggtttc
     7921 gccgtccctt ccatgatgcc cttgatgtcg cggaactggc gccgcacttc gacgaccacg
     7981 gcaccggcgg cccggcccac cgcttccatc gccattgcgc cgaacaggta cggaatgagc
     8041 ccgccgagga aaagcccgat gatcaccatg tggttggaaa ggtcgaacga cgtcgaaagg
     8101 cccgccgtgt cgagcgcatg cgtgtagtcg gcaaagagca ccagcgctgc aagccccgcc
     8161 gagccgatcg catagccctt cgtcaccgcc ttcgtcgtgt tgcccaccgc atcgagcgga
     8221 tcggtgatcg cgcgcaccga ggccggcagg tcggccattt cggcgatgcc gcccgcgttg
     8281 tcggtgatcg gcccgtaggc gtcgagcgcg acgatgatgc cggccatcga cagcatcgcc
     8341 gtcgcggcga ttgcgatgcc gtacaggccc gcgcacagga acgaggcgag gatcgcgatg
     8401 cagacggcga tcaccggcca cgcggtggac ttcatcgata cgccgagccc cgcgatgatg
     8461 ttcgtcgcgt gccccgtcgt ggacgccgcc gcgacatgct tgaccggctt gaactcggtg
     8521 ccggtgtagt actcggtgat gaccaccatc gcagccgtca gcacgagccc gaccagcgac
     8581 gcgccccata tgcgcagcgg cgacagcgcc gcgttcgcgc tgcctatgtc gcccatcatc
     8641 cgctgcgtga tgaagtaaaa gccgattgcg gagatcacgc cggcgacgat gagcccgcga
     8701 tagagcgcgt tcatgatctt gccgccgctg cgcgccttca cgaagtagca gccgacgatc
     8761 gacgcgagaa tcgagaatcc gccgagcacc agcggataag tcacggcggc tgtcgccatc
     8821 gtcggcagca gcagcgcgcc gagcagcatc gtcgcgacga tcgtgaccgc atacgtttcg
     8881 aagaggtcgg cggccatgcc ggcgcaatcg ccgacgttgt cgccgacgtt gtcggcgatc
     8941 accgccgggt tgcgcggatc gtcctccgga atgccggctt cgaccttgcc gacgagatcg
     9001 gcgccgacgt cggcgccctt ggtgaagatg ccgccgccga ggcgcgcgaa gatcgagatc
     9061 agcgagccgc cgaacgcgag gccgacgagc ggctcgatcg ccgcatggag gccttcgttc
     9121 tcgaagttcg gcgcgtagtg ggccgacgcc acgagaaacc agtagaagcc ggcgacgccg
     9181 agcaagccca gcccgaccac cagcatgccg gtgatcgccc ccccgcgaaa cgcgaccgca
     9241 agcgcttcgc caaggccggt gcgcgcggcc tcggccgtgc gcacgttcga gcgcaccgaa
     9301 acgttcatgc cgatgtagcc ggcgagcccc gagaggatcg cgccgaccgc gaagccgccg
     9361 ccggtgcgcc aggagagaaa gatgccgatg agaatgaaca ggatcacgcc gacgatgccg
     9421 atcgtcgtgt attgccggtt gaggtacgcc ttggcgccgg cctggatcgc cgccgcgatt
     9481 tcctgcatgc gggggttgcc cgccggtttg gcgaggaccc agcgaatgga caccgcgccg
     9541 tagacgatcg ccgcaatcgc gcagacgagc gcgaagatga gcgaattgga cattggagac
     9601 tccctttgcg aaacgtgatc ttctggaaca gcgatcggag cgtcgtctgg cggacgcgtg
     9661 ccgatgatgc cggtgcgagc cctccagggg tgcgcatcgg gccgttcgac tgcgcggcgg
     9721 cgccccctgg agggccccgg ccgcaaaaca cgctattttg ccagtttgcg ggcggggaag
     9781 tcgagcctga gcgaagggcg ttcaggtcgg ccgcggtcga cgcgcgacgt caacgaagca
     9841 gcgtcagatc gcgaatcccg ccgcgagctt cttcggcacg gcgacgttct tcagccgaac
     9901 gtagtgcggc aagccgtcac ggtacggcgg cggcgcctcg ccgcgaatga gcggcgcgag
     9961 ataggcgcgc gccctttcgg tgatgccata accgtcgcgc gtgatgaaat tgcgcggcag
    10021 catcttctcg cggttggcga catcggcgag cggtgccacg ccgagcttcc accggtaagg
    10081 acggtcggac acgcgcacga tcgccggcat caccgcattc tgtccgcgca gcgccagctc
    10141 gacggcggcc ttgccgacgg catacgactg gtcgaggtcg gtcttcgacg cgagatggcg
    10201 cgccgcgcgc tgcaggtaat cggcgactgc ccagtgatac ttgtagcgca gccggtcctt
    10261 gacgagcgcc gcgatctgcg ggccgacgcc gcccagctgt gcgtggccga acgcgtcctt
    10321 cgtccctgcc tcgcccatca actcgccctg cgcgttgcgc aggccctcgg atacgccgac
    10381 gcagcaatag ccgaacgtct tgacccgcgc atcgacggcc gccaggaacg ccgcctcgtc
    10441 gaacgcgatc tcgggaaaca gcagcacgag cgggatcggc gtcgcttcgt cgtcggcgag
    10501 cccgacggcc gccgtgatcc agccggcgtg gcggcccatc acctcgagca cgaacacctt
    10561 cgtcgacgtt ttcgccatcg acgcgacgtc gaacgcggcc tcgcgcatcg acgtcgccac
    10621 gtacttcgcg accgagccga agccgggaca gttgtcggtg acggcgaggt cgttgtcgac
    10681 cgtcttcggc acgtgcacgg cgaccagcgg atagtcgagc gcccgagcga tctgcgacac
    10741 cttgaggcac gtatcggcgg aatcgttgcc gccgttgtag aagaagtagc cgatgtcgtg
    10801 cgcgcgaaac acgtcgatca gccgctcgta ttgcgcgcgg ttctcgtcga gcgacctgag
    10861 cttgtagcgg caggagccga acgcgccgcc cggcgtgtgc ctgagtgcgg cgatcgcgcg
    10921 tgcaggctcg cggctcgtgt cgatcaggtc ttcggtcaat gcgccgatga tcccgttgcg
    10981 gccggcatac acgcgaccga tcctcgcgcg gtgcttgcgc gccgtctcga tcacgccggc
    11041 ggcgctcgcg ttgatgacgg ccgtcacgcc acccgactgg gcatagaagg cgttacgtcg
    11101 actggacatg gggtgatcct cctggcttcg agtcggctat ggcagcgccg gcggtccgtt
    11161 ccagcagcgc gagattgcgg gcgagcgacg cgtcgtgcgg acgcacggcg cacgcgaggg
    11221 tctgcgtcgc gcgcgcgtcg tcgacgcgtc ccgccgcgcg ttgcaccatc gcgagcaggg
    11281 tgagcgcaaa cgggtcgttc cggcttgcat cgagcacttc ccggcagacc gcctcggcgc
    11341 ccgcgagatc gcgcgcctgc gcaagatcgg cagcgcgttg caggccgccg ccaacgcgct
    11401 cgacgagcgc atcgtgccgc gccgcccatt ttgcatagat gcggtcgcgg aacgccgcaa
    11461 agcgcgcgtc gtcctggttg acgcccgcgc ccgcgcccga atcgccggcc tcggcacgcc
    11521 actcgaacgt ttgccgcggc tcgaaatgaa aatgcgtgtg ctgcgcgcac tgaatgaaga
    11581 agtcccagtc ctggagaatg tcgagcgact cgtcgaagcg gcatccgcgc gaaacgagct
    11641 cgcgcgagaa cagcgacatc gaaaggtgga tgaagttgcg ctcgaaaagc tcgatcggcg
    11701 cgaacggctg cccgaccggc tgcgtcgtgc catcggcgaa gcgtgcgcgt gcgagcgtgt
    11761 acacgacttt gcaggtcccg gcgttgcgca acgcctcgac gagaccggac acgtgtgccg
    11821 ggagcagcac gtcgtcgtcg tcgagaaagg tcatccagtc gccggtcgcc gcatcgagcc
    11881 cggcgttcgc ggcctgtggc cgggagcgag ccgattcccc gggaacgaag cgcacgcgat
    11941 gattgcctat cgttccgggt ggcaccgggt gtgcggcgcc gcatgccgcg acgatcaccg
    12001 cctcgatgcg ctcgtaatcc tgcatgccga tcgacgccag cgcctcctgc aacgacgccc
    12061 gcgccatgct gcgcacgacg atgcttacga gcggcagcat cggatcgccg cgtttcgcgt
    12121 cagcttcgca acgccgccat gcatgcgtcg cgaatgcctt cgacggtgcc gaggccgttc
    12181 accttgcgat aacgcggcga cttcggatcg ccgctcgctt cccattgcgc gtaataggtg
    12241 acgagcggct cggtttgcgt gtgatagatc gcgagccgct tgcgcacggt ctgttcggag
    12301 tcgtcggcgc gctggatcag cggctcgccg gtgacgtcgt cgcggccggt gatcttcggc
    12361 ggattgaact tgacgtgata ggagcgcccc gacgcaacgt ggatgcgccg cccgctcatg
    12421 cgctcgacga tcgccgcgtc gggtacgtcg atctcgatca cgtagtcgag gacgatgccg
    12481 gcatccttca gcgcatcggc ctgcgggatc gtgcgcggaa agccgtcgag cagatagccc
    12541 tgcgcgcaat cgtcctgttg cagacgctcc ttgac
//
LOCUS       DASSVX010000173        21270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_706803, whole genome shotgun sequence.
ACCESSION   DASSVX010000173 DASSVX010000000
VERSION     DASSVX010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 21270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21270
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706803"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1567)
                     /locus_tag="VFO53_07420"
     CDS             complement(65..1567)
                     /locus_tag="VFO53_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HET8876142.1"
                     /translation="MEIFHHLAIGFGTALTAVNLLYALIGALLGTLIGVLPGIGPVAT
                     IAMLLPTTYALEPVSALIMLAGIYYGAQYGGSTTAILVNMPGESSSVVTCLDGYQMAR
                     KGQAGAALAIAALGSFFAGSCATLLVAGVSLPLSALALKFGPAEYFSLMVLGLIGAVV
                     LAHGSLVKAIAMIILGLLLGIVGTDVNSGVERFAFGIPELSDGIGVVVVAMGLFGFAE
                     IILNLESTEKREVIKTRLKGMWLTKAQLREAAPAVLRGTALGSMLGVLPGGGAMLSSF
                     ASYSIEKKLAKDPSQFGKGAIQGVAGPESANNAGAQTSFIPLLTLGIPENAVMAMMVG
                     AMTIHNIQPGPQVMTSNPQLFWGLIASMWVGNLMLVVLNLPLIGIWIKLLTVPYRMLY
                     PAILLFCAIGVYTVNNTSFDVMQTAFFGFLGVLFAKLECEPAPLLLGFVLGPMMEENL
                     RRALLLSRGDASVFATRPISAGLLAAAILLVLVIAVPNIRRQREEAFQEA"
     gene            complement(1617..2069)
                     /locus_tag="VFO53_07425"
     CDS             complement(1617..2069)
                     /locus_tag="VFO53_07425"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HET8876143.1"
                     /translation="MRVIRNPRDFHAGALFIAIGVATILLSSRYTLGTAARMGPGYFP
                     RILGILLIVLGALLAFRATRVPGARIPAFRWRPTLIVLGSVVLFGAIVRPVGVALSTV
                     ILIVTASAASHEFRPREALVAGVLLAALAVGVFVVGLQLQLPIWPGQD"
     gene            complement(2214..3062)
                     /locus_tag="VFO53_07430"
     CDS             complement(2214..3062)
                     /locus_tag="VFO53_07430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET8876144.1"
                     /translation="MKRTLVAFAVMTALCTGGAFAQAPAAPAAPAKPAAKSAAPAKAA
                     PQQSAAAPEKVLYTQGQYDLLLKERLAQGGQDTPEIRNAVKEELNTRELLSREAKKAG
                     LDKSTDVKTQMDLAAQTVLVRAYVTDWVKKNPIPDADLHKEYDAIKTQIGDKEYKVRH
                     ILVKTEDQAKEIIGELQKGAKFDELAKARSEDPGSKEKGGDLDWNAPANFVKPFGDAM
                     KATPKGKFTPQPVQTQFGWHVIEVDDIRDAKVPSFEEVKPQLQQRLQAQWLDRYFKEL
                     RAKNGV"
     gene            complement(3059..3373)
                     /locus_tag="VFO53_07435"
     CDS             complement(3059..3373)
                     /locus_tag="VFO53_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HET8876145.1"
                     /translation="MSGARPMRAGDAASALEQRLAPLAPARLEIRDDSDKHVGHAGAS
                     SGAGHFSMTIVSEHFLGLTRLARHRAILERVGDLIPYPVHALSIEAYTPDEIRNHERT
                     KQ"
     gene            complement(3370..3900)
                     /locus_tag="VFO53_07440"
     CDS             complement(3370..3900)
                     /locus_tag="VFO53_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HET8876146.1"
                     /translation="MRFLADYFPLLVFFVAFKWQGIWVATAAAIAASVIQIAWFHWRG
                     KVSAVHWLSLVIIVVFGGATLWLRDETFIKWKPTVLYLAFGGVLAAGKLLWHRDLLAV
                     VMKEIALPAPIWTRLTWSWVIFFAAMALANWYIAFHFPTETWVNFKVWGGIGLFLAFA
                     LAQGLFLARHVTEPSR"
     gene            complement(3958..4551)
                     /gene="ampD"
                     /locus_tag="VFO53_07445"
     CDS             complement(3958..4551)
                     /gene="ampD"
                     /locus_tag="VFO53_07445"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HET8876147.1"
                     /translation="MDAAGRTSGAMQVASPNCDDRPEDTPISMIVVHGISLPPGQFGG
                     DAIERLFTNRLDPAAHPAFAAIANVRVSAHYLVRRDGTLVQFVPCTRRAWHAGESAWR
                     GCVRCNDFSVGVELEGADDIAYEPMQYATLGRLARALRRRYPIVDIVGHSDIAPGRKT
                     DPGAAFDWTRLARDLGLRLPGRLGERRRGGSRTPARP"
     gene            4750..7014
                     /locus_tag="VFO53_07450"
     CDS             4750..7014
                     /locus_tag="VFO53_07450"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET8876148.1"
                     /translation="MDSRTAFRRVGLALRFIAPLTLVLALLALALVPLVDALTLRWWV
                     SDLETRSKLVANTMQEQLVDLVEQGNGARVRQLFRRAVEDERLFAVAFCNRDGQIVYR
                     TDTYPVDLGCNEIRGSGPDAHVLRQFPPAPIHVTWFAIPSTGTRIGTLVLVHDMSLVD
                     RRSADTRRYLIGFFILLGVLVSLITVFVAHLSWRGWMAGMRALLRGEGIIQPFRESAN
                     PDLQPLVGEVRALLREVDQSRRNTERGATWNPEMLRSVLRNDLAGDEILLVSNREPYI
                     HMQQGDQVVIRRPASGLVTAMEPIMRACSGTWIAHGSGTADKAMVDRHDHIEVPPDNP
                     SYTLRRVWLTEEEERGYYYGFANEGLWPLCHIAHVRPIFRTSDWERYVEVNQRFAATV
                     CSEARTEDPVVLVQDYHFALLPRFIHEQLPKATIITFWHIPWPNPESFGICPWRARVL
                     DGLLGSTILGFHTQYHCRNFIDTVDRYMEARIEYEASRVSYGGHRTSIEPYPISIQWP
                     SPWAEVQPSVAECRRRVLEREGFPADVRIGIGVDRMDYTKGIIERFHAVEKLLELHRE
                     YVGRFVFVQIAAPSRSSLDDYRMFEEEVHALAARINARFADAAVPPIRLKAEHHEPEQ
                     INEYFRAADICVVTSLHDGMNLVAKEFVAARDDDQGVLLLSQFTGAARELHDALPVNP
                     YHVDQCAEVLDRALRMPPDEQRVRMRSMRRLVQEYNVYRWAGAMLLDASRVRQRQRIV
                     DRIDQHRTRTVEVG"
     gene            7011..7757
                     /gene="otsB"
                     /locus_tag="VFO53_07455"
     CDS             7011..7757
                     /gene="otsB"
                     /locus_tag="VFO53_07455"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET8876149.1"
                     /translation="MIYAFSPEGVACLDAVLATRPLLAFDIDGTLAPIVNRPEDARLP
                     DDVQRCLAVLARHNDVAIITGRSVSDARPMFAFEPRYLIGNHGAEGMPAWRERSAAFA
                     QTVRGWRDALDACASLADSGVTIEDKRYSLSLHYRRAPDRNAARRAIGACIDTLVPPA
                     RIIDGKAVVNVLPPDAPDKGEALRALIGEARCSNVLYVGDDDTDEAVFGLHLPSVLTV
                     RVEAASDSDATLFLRNQAEVLTLLQHIVRH"
     gene            complement(7760..9097)
                     /locus_tag="VFO53_07460"
     CDS             complement(7760..9097)
                     /locus_tag="VFO53_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET8876150.1"
                     /translation="MSRRTRGQPKVLIVDDEPDLLELLEMTLSRMGLDTSRAESVADA
                     QRQLDAQSFDLCLTDWNLPDGEGLRVVEHITQKALDVPVAVITAFGSAENAVAALKAG
                     AFDYLAKPVALEQLRALVKQALKVPEKPQPAASYQLLGESPAMQQVRGMIERLAKSQA
                     PVFINGESGSGKELAARMIHLKGPRADLPFIAVNCGAIPENLMESEFFGYKKGAFTGA
                     EADRDGFFQAATGGTLFLDEVADLPLAMQVKLLRAIQEKKVRKVGSTTEDAVDTRLIS
                     ATHKNLSALVENGEFRQDLYYRLHVIELSMPSLREMREDIPLIATAILTRLARGNAAG
                     LEPEAVIALERYPFPGNVRELENILERGLSLAADPQRITAEDLHLTPVNDESDAPIPA
                     GEKWPLQDYLDRVERAAINEALEKTRYNRTAAAKLLGITFRAMRYRMERLGIK"
     gene            complement(9141..10790)
                     /locus_tag="VFO53_07465"
     CDS             complement(9141..10790)
                     /locus_tag="VFO53_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8876151.1"
                     /translation="MDPSPAVPPYSPPPLPASIVGSSRRILWIVGLFRAVCGALLLGL
                     ALLLDLRSLNVGTPNSFITAASLYFIFGLASFGWIQQERLVLPIAQTLLGLLSGDIFF
                     IALVMIAGGSTGAPLPILLFPQLAASGWLLRTQTAFFHAALAAIVLLGLETWRLAGGD
                     IAGTQLFQTALICLGYFAMVGTAVVIGRYTKASEDLAAQRGIDVANLEHVNRLIIQDM
                     QDGVLVVDLTGVVRGHNAQVTRLLGGFGRMRGGMRLAEFSTTLHDYWRRWNEDFSDPL
                     PPFKIDATQRLLRVRLVRIGSGLNGGTLIYLEDLGRAQNEAQQMKLAAMGRLTASIAH
                     EVRNPLSAINQAAQLLEEDGAVVPEGQRLLSMIRSNAKRIDRIVGEVLQLNRRDRQQP
                     EVIPLGEFVRALTEEIVQAEGIPADGVDIDIADDLLVMFDRGHLNQIAWNLVRNAWQH
                     CRKASGSIRIAARPGYMGDAVICELADDGPGVPTQFRAQIFEPFFTTRPGGTGLGLYI
                     ARELADANGAALELVAKSPGAIFRVTFKRALTPAAAAQAID"
     gene            complement(11023..11274)
                     /locus_tag="VFO53_07470"
     CDS             complement(11023..11274)
                     /locus_tag="VFO53_07470"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HET8876152.1"
                     /translation="MGKIIFWLIVFFVVLLVLRLINAANSRAANGKTRGSSAKTKDTA
                     MTRCVNCGVYLPRTEAVEGPRGPVCGDPQCLPRDARKRG"
     gene            complement(11283..12695)
                     /locus_tag="VFO53_07475"
     CDS             complement(11283..12695)
                     /locus_tag="VFO53_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8876153.1"
                     /translation="MTNTGATTIERLTGIVGAHHVLTSTADVAPYVVDWRGRYHGAAR
                     AVVQPASTAEVAAVVRACADTGVAIVPQGGNTGMCGGATPDAGGGEVVLSLARMRAIR
                     AIDRANATITVDAGVVLADVQRAAHAEGLLFPLSLASEGSCTIGGNLATNAGGTAVLR
                     YGNARELVLGVEAVLADGNVWNGLRGLRKDNTGYDVKQLLIGSEGTLGIITAAVLKLF
                     PSPRSRVTAFAAIAGIAQAVELLRSMQQALGDRLTGFELISGFALHLSRKHHAGSPDP
                     LPGHPWYALLQIDDSADVTLADAVETALASAAERNIVTDATIAQSQEQAARLWALREN
                     ISEAQRREGQNIKHDISLPISAIAQFAAATQTAITSAFPGARVVVFGHLGDGNLHYNV
                     AAPEGCDAREFLANTPQVNRIVHDRVVAAGGSISAEHGIGQLKRDELARYKSPIELAL
                     MRRIKAALDPQGLLNPGKLL"
     gene            12770..13198
                     /locus_tag="VFO53_07480"
     CDS             12770..13198
                     /locus_tag="VFO53_07480"
                     /inference="COORDINATES: protein motif:HMM:NF043076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HET8876154.1"
                     /translation="MVDESDKHRSSAPPLSAQDALAFMQKMWNPFAMPMPGSASGAVT
                     PTEVPSGAPAQPANDASAADAVPGMLAFPNPAAMFAALDPVQIERKIAELKIIEGWLA
                     MSLNLMQMSIKTLELQHASLEAMHGGAARKGKGRAKPPKS"
     gene            complement(13272..15284)
                     /locus_tag="VFO53_07485"
     CDS             complement(13272..15284)
                     /locus_tag="VFO53_07485"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HET8876155.1"
                     /translation="MSLTGRRGLRYGAIAAVVLAALALLVVFFPWNALRGPLASYLGH
                     RLHRGVTINGDLRVRLGFPTLVEVNDLSIANAQWSDLQPMAHATRIALTFDVPSLLHF
                     TPDTIRLSEPRVVLEKNAAGEANWHFGGKGSGAPAFGDISIDGGTLRYRDPTLRGDIT
                     IAVQTATGAANTPDELRFDGQGTLRGDPFTITGHGHGLAALRRVDDPYQLAFDLRAGA
                     TGISFDGTIVPSAPEDLRGALHLRGPDLSELYPLVPSPLPWTPPYNLAGDLTHENDRW
                     NFQRIKGTVGQSDLSGEFALDMSHPRVATIADLTSRRFDYKDLGGFIGLPPGEPGQKA
                     KTPAQRNEVRKRAASSRVLPDKPFDLGKLRQHDVDLRFRGASVKWGRFPLDNLVAHMV
                     LKDGVLHFEPLDFGVADGHVVSNFTLDLTQRQPAAKAQVEIRRVELKRLFPQLASPSG
                     SAGRFGGRAQFSATGNSPADLLASMQGEAAIAMRGGEMSTLQLVLTNLDLARAAALLM
                     RGGDEKAEIHCAIAAMHATRGQLVPDLMVVDSSDELIHGAGSIDFRDEKYDLSLKADS
                     KKPSLVALRGPIMITGTFATPVVRPAVGEAVARIGAAVGLGVLAPPLALLPLIDLGNA
                     PDADCRALYQEAQLASGAKAKSPGTRDNARRTERKSKSPENLARSR"
     gene            complement(15403..17193)
                     /locus_tag="VFO53_07490"
     CDS             complement(15403..17193)
                     /locus_tag="VFO53_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET8876156.1"
                     /translation="MSIISYRITPSNPNAHLFEVRCMLDDPDPAGQRFRLPAWIPGSY
                     LIREFARQFIDVRAEADGVAVNIRKEAKDVWRADPAHGRLTVIASVYAFDLSVRTAYL
                     DATRAYFNGPSVFLCPEGREASACLLDIAPPVGDVYAHWRVAATLDTDGASPHGFGRY
                     RASTYDELIDHPVEMGDFALAAFSAGGVRHEIAVTGRHDCDLERLARDLARMCQCHID
                     LFGAPTPFARYLFQITALGDGHGGLEHRSSTSLLCRRDELPARGDPAINDEYLHFLGL
                     ASHEYFHSWNVKRIKPEAFSPYDLSRENYTRQLWAFEGITSYYDDLALVRCGLIDVTR
                     YLELLGRTVSAVLRTPGRLRQSVADSSFDAWIKFYRSDENSPNAVVSYYTKGSLVALA
                     LDLALRQRKQGSLDSLMRTLWERYGQAGIGVPEDAIERIACEMGGDEMRAFFARYVHG
                     TEDPPLDTLLDAFGIEWHLRPSTGANDGGGKAATGTLPKASLGAKIGNDLKLLHVYSG
                     GAAERAGLAANDVLVAIDGIRASADAVNALMRRRSPGERIPVHAFRRDELVSVDVELL
                     AAPLDTCHLALRTDAPERAVALRNAWLQEA"
     gene            17280..17732
                     /locus_tag="VFO53_07495"
     CDS             17280..17732
                     /locus_tag="VFO53_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET8876157.1"
                     /translation="MRLSDAFVNVWSGRALSVLRIVTGFMFMSHGMQKILGFPVPPPP
                     SFQLLSLTGLAGVLELIGGALVLLGLFTRPVAFILSGEMAFAYFLAHEPHGFWPLVNR
                     GELAVLYCFVFLYLAVAGGGMWSIDHWWRSRRIAPGEALRGRLDARGA"
     gene            17805..18053
                     /locus_tag="VFO53_07500"
                     /pseudo
     CDS             17805..18053
                     /locus_tag="VFO53_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            18196..18471
                     /locus_tag="VFO53_07505"
     CDS             18196..18471
                     /locus_tag="VFO53_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HET8876158.1"
                     /translation="MCRNIKTLFNFDPPATDEEVHAASLQFVRKLSGFNHPSRTNEAA
                     FARAVDDVAAVARTLIKSLVTSAEPRYRDVEAERVRARTLARFGSRG"
     gene            18726..18986
                     /locus_tag="VFO53_07510"
     CDS             18726..18986
                     /locus_tag="VFO53_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876159.1"
                     /translation="MADEVDVTAPRPTIITWKLIGRPQTRFHHTKGIVFEDVRIICKV
                     DEQDDHIYSCTDNAPPGEYKYSIQAIGPGAPPNPKDPWIVNR"
     gene            complement(19410..19748)
                     /locus_tag="VFO53_07515"
     CDS             complement(19410..19748)
                     /locus_tag="VFO53_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876160.1"
                     /translation="MYIAPAAVIDEWKKTPAEKRKEPEEKMMRDWRNWMAGHAKLFAD
                     KGAGVGKTKRITAQGVADARNDIMLYAIVEAESHDAATRAFEGHPHFAIPQASIEVMQ
                     LNPLPGTEGK"
     gene            20079..21244
                     /locus_tag="VFO53_07520"
     CDS             join(20079..20349,20349..21244)
                     /locus_tag="VFO53_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611384.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET8876161.1"
                     /translation="MAAGTRRKFTAEFKREAVKLAAQPGRSVSAVAREVGVERSVLKH
                     WVDNFAAGKYEKDRALPLKAVQQQENERLRRELAKVRMERDILKKAPGLLRQGTDVKF
                     AFVARYRPVWPTRMMCRLLNVSTSGFYEWLGRPPSARSVANASLLVRIRESFALSGQT
                     YGSPRVWKDMVVAGERCSENRIARLMRGALLSGQAKPRRKPTDAGMRPENSIAPNVLE
                     RDFTASAPNRKWAADFTYIWTGEGWLFLAVVIDLFSRRVVGWSMQATMTAQLVLDALV
                     MALWRRGKPTELLHHSDQGSQYASEDFQRLLSAEGIACSMSRRGDCWDNSAIESFFAS
                     LKKERVYRTIYRTRDEGRADIFDYIEAFYNSRRRHSTLGQVSPTEFEQSYAGLT"
BASE COUNT         3669 a         6864 c         7086 g         3651 t
ORIGIN      
        1 gtggcgacgg cgttcgccgc gcaaatcggc tggcgtcgcg acggataggg gagggaccag
       61 tccatcacgc ctcctggaac gcttcctcgc gctggcggcg gatgttcggc acggcaatga
      121 ccagcacgag caggatcgcc gcggcgagca gtccggccga gatcgggcgt gtcgcgaaca
      181 ccgacgcatc cccgcgcgac agcagcagcg cgcggcgcag gttctcctcc atcatcggac
      241 cgagcacgaa gcccagcaac agcggcgccg gctcgcattc gagcttcgcg aacagcacgc
      301 cgagaaagcc gaaaaacgcg gtttgcatca cgtcgaagct cgtattgttg accgtgtaga
      361 cgccgatggc gcagaacagc aggatcgccg ggtagagcat ccggtacggc accgtcagca
      421 gcttgatcca gatgccgatc agcgggaggt tcagcacgac gagcatcagg ttgccgaccc
      481 acatcgacgc gatgagtccc cagaagagct gcggattcga cgtcatcacc tgcgggcccg
      541 gctggatgtt atggatggtc attgcgccga ccatcatcgc catcaccgcg ttctccggga
      601 tgccgagcgt cagcagcgga atgaacgatg tctgcgcgcc cgcgttgttc gccgactcgg
      661 gtccggcgac gccctggatc gcgcccttgc cgaactgcga cggatccttc gcgagttttt
      721 tctcgatgct gtacgacgcg aacgacgaca gcatcgcgcc gccacccggc agcacgccga
      781 gcatcgagcc gagtgccgtg ccccgcagca ccgccggagc cgcctcgcgc aactgcgcct
      841 tcgtgagcca catccccttg agccgggtct tgatcacctc gcgcttctcg gtcgattcga
      901 gattgaggat gatctcggcg aacccgaaca gtcccatcgc gacgacgacg acgccgatgc
      961 cgtccgacag ttcggggatg ccgaaggcga aacgctcgac accggaattg acgtcggtgc
     1021 cgacgatgcc gagcagcagc cccaggatga tcatcgcgat ggccttcacg agcgagccgt
     1081 gcgcgagtac cacggcgccg atcaggccga gaaccatcag cgaaaagtat tccgccggac
     1141 cgaacttgag cgcgagtgcc gacagtggca gcgatacgcc ggcgacgagc agcgtcgcgc
     1201 acgaacccgc gaagaacgag ccgagcgccg cgatcgcgag cgcggctcct gcctggccct
     1261 tgcgtgccat ctggtagccg tcgaggcacg tgaccaccga cgaggactcg ccgggcatgt
     1321 tgacgaggat ggccgtggtc gagccgccgt actgcgcgcc gtaatagatg ccggccagca
     1381 tgatcagcgc cgacaccggc tccagcgcat aggtcgtggg cagcagcatg gcgatcgtcg
     1441 cgacgggacc gatgccgggc agcacgccga tcagcgtgcc gagcagcgcg ccgatcaacg
     1501 cgtagagcag gttgactgcg gtgagcgcgg tgccgaagcc gatcgcgaga tggtgaaaga
     1561 tttccatgcg ctcgaacgcg agggggtcgc tgggcaggtg gtgcgaacga agagagctag
     1621 tcctggcccg gccagatcgg aagctgcaac tgcaggccga cgacgaagac gccgaccgcg
     1681 agcgctgcga gcagcacgcc cgcgacgagc gcttcacgcg ggcggaactc gtggcttgcg
     1741 gcgctcgcgg tcacgatcag gatgacggtg gaaagcgcga ccccgacggg tcgcacgatc
     1801 gcgccgaaca gcaccacgct gccgaggacg atcagtgtcg gccgccagcg aaaggcggga
     1861 atgcgcgcgc cgggaacgcg cgtggcgcga aacgcaagca gggcaccgag aacgatgagc
     1921 aggatgccga ggatgcgcgg gaaataaccc gggcccatgc gtgccgccgt acccagcgtg
     1981 tagcggctgc tgagaaggat cgtcgcgacg ccgatggcga tgaagagcgc gccagcatgg
     2041 aaatccctgg gattacgaat cacccgcatc acacttccgt atcgtgcagc cgccgttcac
     2101 ccatcgtggc cgaggcggct cttttagcat agcaccgggg ccggtgcacg aaaggccaca
     2161 aaaaagcagg ctcgttgcca agcctgcctt tgccgtgccg gaccgcgttg cggtcacacg
     2221 ccgttcttcg cccggagctc cttgaagtac ctgtcgagcc attgcgcctg caggcgttgc
     2281 tggagctgcg gcttcacttc ctcgaagctc ggcaccttcg cgtcgcgaat gtcgtcgact
     2341 tcgatcacat gccagccgaa ttgcgtctgc accggctgcg gcgtgaactt gcccttcggc
     2401 gtcgccttca tcgcgtcgcc gaacggcttc acgaagttgg cgggcgcatt ccagtcgagg
     2461 tcgccgccct tttccttgga gccgggatcc tccgaccgtg ccttggcaag ctcgtcgaac
     2521 ttcgcgccct tctgcagctc gccgatgatt tccttggcct ggtcttcggt cttcacgagg
     2581 atgtggcgca ccttgtattc cttgtcgccg atctgcgtct tgatcgcgtc gtattccttg
     2641 tgcagatcgg cgtcggggat cggattcttc ttgacccagt cggtgacgta cgcgcgcacg
     2701 agcaccgttt gcgccgcgag gtccatctgc gtcttcacat ccgtgctctt gtcgaggccc
     2761 gccttcttgg cttcacgcga gagcagctcg cgcgtattga gctcttcctt caccgcgttg
     2821 cggatctcgg gagtgtcctg tccgccctgc gcgaggcgtt ccttcagcag cagatcgtac
     2881 tgcccctgcg tatagagaac cttctcgggc gctgcggcgg actgctgcgg cgccgccttc
     2941 gccggcgcgg cggacttggc cgcgggcttg gcgggtgcag cgggtgccgc gggagcctgc
     3001 gcgaacgcgc cacccgtgca aagcgcggtc atgaccgcaa aggcaaccaa cgtacgtttc
     3061 attgcttcgt cctttcgtga ttacggattt cgtccggggt gtacgcctcg atcgagagcg
     3121 cgtgaaccgg ataggggata aggtcgccga cgcgctcgag gatggcccgg tggcgggcca
     3181 ggcgggtcag cccgaggaag tgctccgaca cgatcgtcat cgaaaagtgc cccgcacccg
     3241 aagatgcgcc ggcgtgaccc acgtgcttgt cgctgtcgtc gcgaatttcg agccgcgccg
     3301 gcgcaagcgg cgcaagccgc tgctcgagcg cgctcgcggc atcgccggcg cgcatcggcc
     3361 gcgccccgct catcgtgacg gctcggtcac gtgacgggcg aggaacaggc cctgcgcgag
     3421 cgcgaacgcg aggaacaggc cgataccgcc ccagaccttg aagttgaccc atgtctcggt
     3481 cgggaaatga aacgcgatgt accagtttgc aagcgccatt gccgcgaaaa agattaccca
     3541 cgaccacgtg agccgcgtcc agatcggcgc gggcaacgcg atctccttca tcacgacggc
     3601 gagcagatcg cggtgccaca ggagcttgcc cgccgcgaga acgccgccga acgcaaggta
     3661 gagcaccgtc ggcttccact tgatgaacgt ctcgtcgcga agccagagcg tggcgccgcc
     3721 gaagacgacg atgatcacga gcgagagcca gtgcacggcc gagaccttgc cgcgccaatg
     3781 gaaccacgcg atctggataa cggacgcggc gatggccgct gccgtggcaa cccagatccc
     3841 ctgccacttg aatgcgacga agaaaacgag cagcggaaag tagtccgcga gaaagcgcat
     3901 aaacagtcga ttataccttg tcttttgggc gtgccggccg caccggcatt gccggattca
     3961 cgggcgcgcc ggcgttcggc tgccgccgcg ccgccgctcg cccaggcggc ccggcaagcg
     4021 caagccgagg tcgcgtgcaa ggcgcgtcca gtcgaatgca gcgccggggt ccgtcttccg
     4081 gccgggggcg atgtcgctgt gcccgacgat gtcgacgatc ggatagcgcc gccgcaacgc
     4141 gcgcgcgagc cggccgagtg ttgcgtattg catcggctcg tacgcgatgt cgtcggcgcc
     4201 ttcgagctcc acgccgaccg agaaatcgtt gcagcgcacg cagccccgcc acgccgattc
     4261 gcccgcgtgc cacgcgcgcc gcgtgcaggg aacgaactgc acgagggtgc cgtcgcggcg
     4321 cacgagatag tgcgccgaga cgcgcacatt ggcgatcgct gcgaacgcgg gatgcgcggc
     4381 cgggtcgagg cggttcgtga agagccgctc gatcgcatcg ccgccgaact gaccgggcgg
     4441 cagtgaaatg ccgtgcacga cgatcatcga gatcggcgta tcttcgggtc tgtcgtcgca
     4501 gttcggcgat gcgacctgca tggcgccgct cgtgcgccct gcggcgtcga tggcgagccg
     4561 ggtcgcacgc cggcgtaccg gtgcgccggc cgggcggtcc aggcgggcag gttgtcgtct
     4621 cttcacgatg ggcgcgggat tttgccataa ccccggcgcg gaaactctcc ggcctgtccg
     4681 gctgcccaac catcccggcc cgatccatcc cgaccagcca cgcgaggtcc cgaaagccgc
     4741 cttcgaacga tggattccag gacagccttc cgccgggtcg gactcgcact gcgcttcatc
     4801 gcgccgctca cgctggtgct ggcgctcctg gcgctcgcgc tggtgccgct cgtcgatgcg
     4861 ctgacgctgc gctggtgggt ctccgacctg gaaacccgat cgaagctcgt ggccaacacg
     4921 atgcaggagc agctcgtcga cctggtcgag cagggcaacg gcgcacgcgt gcggcagctt
     4981 tttcgccgcg cggtcgagga cgagcgattg ttcgcggtcg cgttctgcaa ccgtgacggg
     5041 cagatcgtct accgcaccga cacgtatccg gtcgatctcg gctgcaacga aatcaggggc
     5101 agcgggccgg acgcgcacgt gctgcggcag ttcccgccgg cgccaatcca cgtgacgtgg
     5161 tttgcgatcc cgtcgacggg aacgcgaatc ggcacgctcg tgctcgtgca cgacatgagc
     5221 ctcgtcgacc gccgcagcgc cgacacgcgc aggtacctca tcggcttctt catcctgctc
     5281 ggcgtgctcg tgtcgctgat caccgtgttc gtcgcgcacc tttcgtggcg cggctggatg
     5341 gccggcatgc gcgcgctcct gcgcggcgaa gggatcatcc agccatttcg cgaatcggcc
     5401 aatcccgacc tgcagccact ggtcggcgag gtgcgggcgc tgctgcgcga agtcgaccag
     5461 agccggcgca ataccgagcg cggcgccacg tggaatccgg agatgctgcg ctcggtgcta
     5521 cgcaacgatc tcgccggcga cgagatcctg ctcgtgtcca accgcgagcc gtacattcac
     5581 atgcaacagg gagatcaggt cgtgatccgg cgtccggcaa gcggcctcgt cactgccatg
     5641 gagccgatca tgcgcgcctg ctcgggcacc tggattgcgc acggcagcgg caccgccgac
     5701 aaggcgatgg tcgaccgcca cgatcatatc gaggtgccac ccgacaatcc gagctacacg
     5761 ctgcgccgcg tctggctgac cgaggaagag gagcgcgggt actactacgg tttcgccaac
     5821 gaaggattgt ggccgctgtg ccacatcgcg cacgtgcggc cgatctttcg caccagcgac
     5881 tgggagcgct acgtcgaggt gaaccagcgc ttcgccgcga ccgtgtgctc ggaggcgcgc
     5941 accgaggatc ccgtggtcct cgtgcaggac taccacttcg cgctgctgcc gcgcttcatc
     6001 cacgagcagc tgccgaaggc gacgatcatc actttctggc acatcccctg gcccaatccc
     6061 gagtcgttcg gcatctgccc gtggcgcgcg cgcgtgctcg atggcctcct cggcagcacg
     6121 atcctcggct tccatacgca ataccattgc cgcaatttca tcgacaccgt cgaccgctac
     6181 atggaggcgc gcatcgaata cgaggcatcg cgcgtttcgt acggaggaca tcgaacgagc
     6241 atcgagccgt acccgatctc gatccaatgg ccgtcgccat gggccgaggt gcagccgagc
     6301 gtggccgagt gccgccggcg cgtgctcgag cgcgaaggct ttccggccga tgtgcgcatc
     6361 ggcatcggcg tcgatcgcat ggattacaca aaagggatca tcgagcgctt ccacgcggtg
     6421 gaaaagctgc tcgagctgca ccgcgagtac gttggccggt tcgtgttcgt gcaaatcgcg
     6481 gcgccttcgc gctcgtcgct cgacgactac cggatgttcg aggaggaggt gcatgcgctc
     6541 gccgcgcgga tcaacgcacg cttcgccgac gccgccgtgc cgccgatacg cctcaaggcc
     6601 gagcatcacg agccggagca gatcaacgag tacttccgcg cggcggacat ctgcgtggtc
     6661 acgagcctgc acgacgggat gaacctcgtc gccaaggaat tcgtcgctgc gcgcgacgac
     6721 gaccagggcg tgctgctgct ctcgcagttc accggcgccg cacgcgaatt gcacgacgca
     6781 ctgcccgtga acccgtatca cgtcgatcag tgcgccgagg tcctcgaccg tgcgctgcgg
     6841 atgccgcccg acgaacagcg ggtgcgcatg cgcagcatgc ggcggctcgt tcaggagtac
     6901 aacgtgtatc gctgggcggg cgcgatgctg ctcgatgcga gccgtgtgcg ccagcggcag
     6961 cggatcgtcg atcgcatcga ccagcaccgc acgcggacgg tggaagtcgg ttgatctacg
     7021 ctttttcacc cgaaggcgtc gcctgtcttg acgccgtcct cgcgacgagg ccgctgctcg
     7081 cattcgacat cgacggcacg ctcgcgccga tcgtcaatcg tccggaagac gcgcggctgc
     7141 ccgacgacgt gcaacgctgt cttgcagtac ttgcgcggca caacgacgtt gcgatcatca
     7201 ccgggcgctc ggtgagcgat gcacgcccca tgttcgcgtt cgagccgcgc tatctgatcg
     7261 gcaaccatgg cgccgaaggc atgccggcgt ggcgcgagcg ctcggccgca ttcgcgcaga
     7321 cggtgcgcgg ctggcgcgac gcgctcgacg catgcgcgtc acttgcagac agcggcgtaa
     7381 ccatcgagga caagcggtat tcgctctcgc tgcattacag acgggcgccg gacaggaacg
     7441 cggcccgccg cgcgatcggc gcatgcatcg acacgctcgt cccgccggcc aggatcatcg
     7501 acggcaaggc ggtggtgaac gtgctcccgc ccgatgcgcc ggacaagggc gaggcgctgc
     7561 gcgccttgat cggcgaggcc cgctgctcca acgtgctcta cgtgggcgac gacgacaccg
     7621 acgaggcggt gttcggcttg cacctgccga gcgttctgac ggtgcgcgtc gaggctgcgt
     7681 ccgacagcga tgcgacgctg ttcctgcgca atcaggccga ggtgctgacg ctgctgcagc
     7741 acatcgtgcg gcactgagcc tacttgatcc ccagccgttc catccggtag cgcatcgctc
     7801 tgaacgtgat cccgagcagc ttcgcggccg ccgtgcggtt gtaacgcgtc ttttcgaggg
     7861 cctcgttgat cgccgcgcgc tcgacgcggt cgagatagtc ctgcagcggc cacttctcgc
     7921 cggcgggaat cggcgcatcc gattcatcgt tgacgggcgt gaggtgcaga tcctcggcgg
     7981 tgattcgctg cggatcggcg gcaagcgaca ggccgcgctc gaggatgttc tcgagctcgc
     8041 gcacgttgcc cgggaagggg tagcgctcga gtgcgatgac cgcctcgggc tcgagtcccg
     8101 cggcgttgcc gcgcgcgagc cgcgtgagga tggcggttgc gatcagcgga atgtcctcgc
     8161 gcatttcgcg caacgaaggc atcgacagct cgatcacgtg cagccggtag tacagatcct
     8221 ggcgaaactc gccattctcg acgagcgccg acaggttctt gtgcgtggcg ctgatgagcc
     8281 gcgtgtcgac ggcgtcttcg gtggtcgagc cgaccttgcg caccttcttt tcctggatcg
     8341 cgcgcagcag cttgacctgc atcgcgagcg gcaggtcggc cacttcgtcg aggaacagcg
     8401 tgccaccggt cgcggcctgg aagaacccgt cgcgatcggc ttcggcgccg gtgaacgcgc
     8461 ccttcttgta gccgaagaac tcgctctcca tcaggttctc gggaatggcg ccgcagttga
     8521 ccgcgatgaa cgggaggtcg gcgcgcggac ccttgagatg aatcatgcgc gccgcgagct
     8581 ccttgccgct gcctgactcg ccgttgatga acaccggcgc ctggcttttc gcgagccgct
     8641 cgatcatccc gcgcacctgt tgcatcgccg gcgattcgcc gaggagctgg tagctcgccg
     8701 ccggttgcgg cttttcgggc accttgagcg cctgcttgac cagtgcgcgc agctgttcga
     8761 gtgcgaccgg cttcgcgagg tagtcgaagg cgccggcctt gagcgccgcg acagcgttct
     8821 ccgcactgcc gaatgcggtg atcacggcca ccggcacgtc cagcgccttc tgcgtgatgt
     8881 gctcgacgac gcgcagtccc tcgccgtcgg gcaggttcca gtcggtgagg cacaggtcga
     8941 aggactgcgc gtcgagctga cgctgggcgt cggctaccga ctcggcgcgc gacgtgtcga
     9001 ggcccatccg cgacagcgtc atttcgagca gttcgagaag atcgggctcg tcgtcgacga
     9061 tcaggacctt cggttgacca cgggttctgc gggacatggg gataggcgat cgtgaggaaa
     9121 gtggcgggca gcggcggatt ctagtcgatc gcctgcgcgg cggccgccgg tgtcaacgcg
     9181 cgcttgaacg tcacgcggaa aatggcgccc ggcgatttgg caacgagctc gagcgccgcg
     9241 ccgtttgcat cggcaagctc gcgtgcaata tacagaccaa gcccggttcc gccgggtcgc
     9301 gtcgtgaaga acggctcgaa gatctgcgcg cggaattgcg tcggcacgcc cggcccgtcg
     9361 tcggcgagct cgcagatcac cgcgtcgccc atgtaacccg gacgcgccgc gatgcggatg
     9421 ctgccgctgg ccttgcggca gtgctgccac gcgttgcgca cgagattcca tgcgatctgg
     9481 ttcaggtggc cgcgatcgaa catcacgagc agatcgtcag cgatgtcgat atcgacccca
     9541 tccgcgggaa tgccttccgc ctggacgatt tcctcggtga gcgcgcgcac gaactcgccg
     9601 agcgggatca cttcgggctg ctggcggtcg cgccggttga gctgcagcac ctcgccgacg
     9661 atgcggtcga tgcgctttgc attgctgcgg atcatgctga gcagccgctg cccctcgggc
     9721 accactgcgc cgtcctcctc gagcaactgc gccgcctggt tgattgccga aagcggattg
     9781 cgcacctcgt gggcgatcga cgcggtgagc cggcccatgg cagcgagctt catctgctgc
     9841 gcctcgttct gcgcgcggcc gagatcctcg agatagatga gcgtgccgcc gttcagcccc
     9901 gagccgatgc gcacgagccg cacgcgcagc agccgctgcg tcgcgtcgat cttgaacggt
     9961 ggcagcggat cggaaaaatc ctcgttccag cgccgccagt agtcgtgcag cgtcgtcgag
    10021 aactcggcga ggcgcatgcc gccgcgcatg cggccaaacc cgccgagcag ccgcgtgacc
    10081 tgcgcgttgt gcccgcgcac cacgccggtg agatcgacga cgagcacgcc gtcctgcatg
    10141 tcctggatga tcagccggtt gacgtgctcg aggttggcga cgtcgatgcc gcgctgtgcc
    10201 gcgaggtcct cggacgcctt ggtgtagcgc ccgatgacga ccgcggtgcc gaccatcgcg
    10261 aagtagccga ggcagatcag cgccgtctgg aacagttgcg tgccggcaat gtcgccgccc
    10321 gcgaggcgcc aggtttcgag gccgagcagg acgatggcgg cgagcgctgc gtgaaagaac
    10381 gccgtttgcg tgcgcagcag ccagccgctc gccgccagct ggggaaagag caggatgggc
    10441 agcggtgcgc cggtgctgcc gccggcgatc atcacgagcg cgatgaagaa gatgtcgccc
    10501 gagagcagcc cgagcagcgt ttgcgcgatc ggcagcacca gccgttcctg ctggatccag
    10561 ccgaacgaag cgagcccgaa gatgaaatag agcgacgccg cggtgatgaa cgaattgggc
    10621 gtgccgacgt tgagcgagcg aagatcgagc agcagcgcaa ggccgagcag cagcgcaccg
    10681 cacaccgcac gaaaaagtcc gacaatccac aggatccgcc gcgacgagcc gacgatggat
    10741 gcaggcagcg ggggcggact gtacggcgga acggcgggcg agggatccac ggtcggttgc
    10801 gcgcgggcgg cacccgcgat gttcagccag cgccggcggt tgcccgtgcg gcggctcgcg
    10861 cattgcgcgg cgcgctgcac ggcgcgcggc ctgcggctcg cgcgaattgt gccagtaacg
    10921 gagcggcggc gggagaactt tgggcgccgc ggtgcgcgat cgacgcgccg gcgtggatcg
    10981 gacgcaacac ttgccggccc gggaatgcac cggcgcggct gttcaaccgc gcttgcgcgc
    11041 gtccctcggc aggcactgcg ggtcgccgca gacggggccg cgcggccctt ccacggcctc
    11101 cgtgcgcggc aggtacacgc cacagttcac gcagcgcgtc atcgccgtgt ccttcgtctt
    11161 cgccgaactt ccccgcgtct tgccattcgc tgcgcggctg ttggcggcgt tgatgagccg
    11221 cagcaccagc agcacgacga agaagacgat cagccagaag atgatcttgc ccatgtctcg
    11281 cgctacagca gcttgccggg attgagaagc ccctgcgggt cgagcgccgc cttgatgcgc
    11341 cgcatcaacg cgagctcgat cgggctcttg taacgcgcga gctcgtcgcg tttcaactgt
    11401 ccgatgccgt gctcggcgct gatgctgccg ccggccgcga cgacgcgatc atggacgatg
    11461 cggttgacct gcggcgtgtt cgcgaggaat tcgcgcgcat cgcaaccctc gggtgccgcc
    11521 acgttgtaat gcaggttgcc gtcgccgaga tgaccgaaca cgacgacgcg cgcgccgggg
    11581 aacgcgctcg tgatcgcggt ttgcgtggcg gcggcgaatt gcgcaattgc cgagatcggc
    11641 agcgaaatgt cgtgcttgat gttctgcccc tcgcgccgtt gcgcctcgct gatgttctcg
    11701 cgcagcgccc acagccgcgc ggcctgctcc tgcgactgtg cgatagtcgc atccgtgacg
    11761 atgttgcgct ccgcggcgct ggcgagcgcg gtttccaccg catcggcaag cgttacgtcg
    11821 gcgctgtcgt cgatttgaag cagcgcgtac cacgggtggc cgggtagcgg gtcgggcgac
    11881 ccggcatggt gcttgcgcga caggtgcaat gcaaagccgc tgatgagctc gaacccggtg
    11941 agccgatcgc ccagcgcctg ctgcatcgac ctcaacaatt cgacggcctg cgcgatgccg
    12001 gcgatggcgg cgaacgcggt cacgcgcgaa cgcggcgacg ggaacagctt cagcacggcc
    12061 gccgtgatga tgccgagcgt accttcgctg ccgatgagca actgcttgac gtcgtacccg
    12121 gtgttgtcct tgcgcaggcc ccgcaggccg ttccagacgt tgccgtcggc aagcacggcc
    12181 tccacgccga gcacgagctc acgcgcgttg ccgtaacgca gcacggccgt gccacccgcg
    12241 ttcgtcgcca ggttgccgcc gatggtgcag cttccttccg aggcgagcga tagcggaaac
    12301 agcaatccct cggcatgtgc cgcccgctgc acatccgcga gaacgacgcc cgcgtccaca
    12361 gtgatcgtgg cgttggcgcg atcgatggca cggatcgccc gcatccgcgc aagcgacagc
    12421 acgacttctc cgccgcccgc atccggcgtc gcgccgccgc acatgccggt attgcctcct
    12481 tgcggcacga tcgcgacgcc ggtgtcggcg cacgcgcgca ccaccgcggc cacttcggcc
    12541 gtgctcgccg gttgcaccac cgcgcgcgca gcgccgtgat agcggccgcg ccagtcgacg
    12601 acgtacggcg caacgtccgc ggtgctcgtc aatacgtgat gcgcgcccac gatgccagtc
    12661 agtcgctcga tcgtggttgc gccggtgttc gtcatggcgc acgatattag cgcaccgtgc
    12721 gcgccgcttc gcaaacggtg gttatcgggt gccgcgcagg agcgacgaca tggtcgacga
    12781 atcggataag catcgcagtt cggcgccgcc gttgtcggcg caggatgcgc tggctttcat
    12841 gcagaagatg tggaatccgt tcgcgatgcc gatgccgggc tcggcgagcg gtgccgtgac
    12901 gcccaccgag gtgccatcgg gagccccggc ccagccggcg aacgatgcct cggccgccga
    12961 tgccgtgccg gggatgctcg cgttccccaa tcctgcggcg atgttcgcgg cgctcgatcc
    13021 ggtgcagatc gagcgcaaga tcgccgagct caaaatcatc gaggggtggc ttgcgatgag
    13081 cctcaacctg atgcagatga gcatcaagac gctggagctg cagcatgcat cgctcgaggc
    13141 gatgcacggc ggtgccgcgc gcaagggcaa aggccgcgcg aagccgccca aatcctgacg
    13201 cgccgcgctg accggcgcga ttacgaaggg tgcgcgctcg tacaccggca cgcgtcgcgc
    13261 cggtcgagcc gctagcgcga gcgggcaagg ttctccgggg atttcgactt gcgttcggtg
    13321 cgtcgggcgt tgtctcgcgt gccgggcgac ttcgctttcg cgcccgaggc gagctgcgcc
    13381 tcctgatata gcgcccggca gtccgcgtcg ggggcattgc caaggtcgat gagcggcagc
    13441 agcgcgaggg gcggcgccag aacgccgaga ccaaccgctg caccgatccg cgcaaccgcc
    13501 tcgccgaccg ccggacgcac caccggcgtt gcaaaggtgc cggtgatcat gatcggcccg
    13561 cgcagcgcga caaggctcgg cttcttcgaa tcggccttga gcgacaggtc gtatttttcg
    13621 tctcgaaagt cgatgctgcc cgcgccatgg atcaattcat cggaactgtc gacgaccatg
    13681 agatcgggga cgagctggcc gcgcgtcgcg tgcatcgccg cgatcgcgca atggatctcg
    13741 gctttctcgt cgccaccgcg catgagcagc gccgcggcac gcgcgagatc caggttcgtg
    13801 agaacgagct gcagcgtgct catctcgccg ccacgcattg cgatcgccgc ctcgccctgc
    13861 atcgacgcca gcagatcggc ggggctgttg ccggttgcgc tgaactgcgc tcgcccgccg
    13921 aagcggccgg ccgatccgct gggcgaggcg agctggggga aaaggcgctt cagctcgaca
    13981 cgccgtattt cgacctgcgc cttcgccgcc ggctgacgtt gggtgaggtc gagcgtgaag
    14041 ttgcttacga cgtgcccgtc ggccacgccg aagtcgagcg gctcgaagtg cagcacgccg
    14101 tccttgagaa ccatgtgcgc gacgagattg tcgagcggga agcggcccca cttgacgctt
    14161 gcgccgcgaa acctgaggtc gacgtcgtgc tggcgcagct tgccgaggtc gaacggcttg
    14221 tcgggcagca cccgcgacga cgcggcccgc ttccgcacct cgttgcgctg cgccggcgtc
    14281 ttcgccttct ggcccggttc acccggcggc aagccgatga atccgccgag atccttgtag
    14341 tcgaacctgc gcgaggtcaa gtcggcgatc gtcgccaccc gcgggtgcga catgtcgagc
    14401 gcgaactcgc cactgaggtc gctttggcca accgttccct tgatgcgctg gaagttccag
    14461 cgatcgttct cgtgcgtgag gtcgcccgcg aggttgtacg ggggcgtcca cggaagcggt
    14521 gaaggcacga gcggatagag ttccgacaga tcggggccgc gcaggtgcaa cgcgccgcga
    14581 agatcttcgg gagcggaggg aacgatcgtg ccgtcgaagg aaatgccggt tgcgccggca
    14641 cgcaggtcga acgcgagctg atatggatcg tcgacccggc gcagcgctgc aaggccatgg
    14701 ccgtggcccg tgatcgtgaa ggggtcgccg cgcagcgtgc cttggccatc gaagcgcaat
    14761 tcgtcgggcg tattcgccgc gccggtggcc gtctgcacgg cgatggtgat gtcgccgcgc
    14821 agcgtcgggt cgcgatagcg cagcgtccca ccgtcgatgc tgatatcgcc gaacgccggc
    14881 gcacccgatc ccttgccgcc gaaatgccag ttcgcttcgc cggccgcatt cttctcgagc
    14941 accacccgtg gttcgctcag gcggatcgtg tcgggcgtga agtgaagcag ggacggaacg
    15001 tcgaacgtga gtgcgatgcg cgtggcgtgt gccatcggct gcagatcgga ccactgcgca
    15061 ttggcaatcg aaagatcgtt gacttcgacg agcgtgggga acccgaggcg gacgcgcagg
    15121 tcgccattga tggtcacgcc tcgatgcagg cggtgaccga ggtacgaggc gagtggcccc
    15181 cgcagtgcgt tccacgggaa gaatacgaca agcagcgcaa gcgccgcgag aacgacggcc
    15241 gcgattgcac cgtatcgaag gccgcgccgg ccggtaagtg acatgaaaga tcgcgtcgac
    15301 gagcgcgcgc gaacgcacga acgttaccga gaaagtcgaa gcgtacgggt gccccggggc
    15361 aacggcaatc cgccgcttgc gcagcgttgc gtaagaatgg tcttacgctt cctggagcca
    15421 ggcgtttcgc agcgcgactg cgcgctcggg ggcgtccgtt cgcaatgcaa gatggcaggt
    15481 gtcgagcggt gcggccagca gctcgacgtc gacgctcacc agctcatcgc ggcgaaacgc
    15541 gtgcacaggg atacgttccc cgggcgagcg gcgccgcatc agcgcattga ccgcgtcggc
    15601 ggaggcgcga atgccgtcga tggcgaccag cacgtcgttc gctgcgaggc cggcacgctc
    15661 ggccgcaccg ccgctgtata catgcagaag cttcaggtcg ttgccgattt tcgcgccgag
    15721 cgaggctttc ggcagcgtcc cggttgcggc cttgccgccg ccatcgtttg caccggtcga
    15781 gggacgcaga tgccattcga ttccgaaggc gtcgagcaac gtatcgagcg gcgggtcctc
    15841 ggtgccgtgc acgtaccgcg cgaaaaacgc gcgcatctcg tcgccgccca tctcgcaggc
    15901 gatgcgctcg atcgcgtcct cgggaacgcc gatgcctgcc tgcccgtaac gctcccacag
    15961 cgttcgcatc agcgagtcga gcgagccctg cttacgttga cgcagtgcca agtccagcgc
    16021 aagtgcgacg agcgagccct tcgtgtagta gctgacgacc gcattcggag agttttcgtc
    16081 ggaacgatag aacttgatcc acgcgtcgaa gctcgaatcg gcgacgctct gccgcaagcg
    16141 gccaggtgtg cgcaacaccg cgctgaccgt gcgccccagc agctccagat agcgcgttac
    16201 gtcgatcagg ccacagcgga cgagtgcaag gtcgtcgtag tacgaggtga tgccctcgaa
    16261 ggcccagagc tgccgcgtgt agttttcgcg cgaaaggtcg tacggggaga acgcctccgg
    16321 cttgatccgt ttcacgttcc atgaatggaa atattcgtgg ctcgcgagac cgaggaagtg
    16381 cagatattcg tcgttgatcg ccgggtcacc gcgcgccggc agctcgtcgc ggcggcagag
    16441 gaggctcgtg ctcgaccggt gctcgagacc gccgtggcca tcgccaagcg cggtaatctg
    16501 gaacaggtaa cgcgcgaagg gtgtgggagc gccgaacaga tcgatgtggc attggcacat
    16561 gcgcgcgaga tcgcgtgcga gccgctcgag gtcgcaatcg tggcggccgg tgaccgcgat
    16621 ttcatggcgc acgccgcccg cgctgaaggc cgcgagcgcg aagtcaccca tctcgaccgg
    16681 atgatcgatg agctcgtcgt acgtcgacgc gcgatagcgg ccgaacccat gaggggatgc
    16741 gccgtccgtg tcgagcgtgg cggcgacgcg ccaatgcgca taaacgtcgc ccacgggcgg
    16801 cgcgatatcc agcaaacagg ccgaagcctc gcgtccttcc gggcacagga agacgctggg
    16861 tccgttgaaa taggcgcgcg tggcgtcgag ataggcggta cgcacggaaa ggtcgaacgc
    16921 gtaaacgctg gcgatcaccg tgagcctgcc gtgggctggg tctgcgcgcc acacgtcctt
    16981 ggcttccttg cggatattta ccgccactcc atccgcttcc gcgcgcacat cgatgaactg
    17041 acgggcgaat tcgcgtatca aatagcttcc gggaatccac gcgggcaggc ggaagcgttg
    17101 tcccgcgggg tcgggatcgt cgagcatgca tcgcacctcg aatagatgcg cattcggatt
    17161 gctcggcgtg atgcggtagg aaatgatcga catgtcgagg gctctacgag gcggcgcagc
    17221 caacaacacc tgcacgattg cggcgcagtg gcacaaccat tttcaggagg taacgggcaa
    17281 tgagactttc cgatgcattc gtcaacgtct ggtccggccg ggcgctcagc gtgcttcgca
    17341 ttgtcaccgg tttcatgttc atgtcgcacg gcatgcagaa gatccttggt ttccccgtgc
    17401 cgccgccccc gtcgttccag ctcttgtcac tcaccggcct cgctggcgtg ctcgaattga
    17461 tcggcggtgc cctggtgctt cttgggctct tcacgcgccc ggtcgcattc atcctgtccg
    17521 gggaaatggc gttcgcctat tttctcgcac atgagccgca tggtttctgg ccactcgtca
    17581 atcgcggaga gctcgcagtc ctctattgct tcgtttttct ctacctcgcg gtcgccggag
    17641 gtggaatgtg gagcatcgac cattggtggc gcagtcgaag gatcgctccg ggtgaagcct
    17701 tgcgcgggcg ccttgacgca cgcggtgctt gacgccgacg tccgagatcg gccgccagcg
    17761 acgttggtac gcgagtagat caagcagttt gcctcgtcgt agcgacgtgt ccaacagcag
    17821 gctcaggttc ctccaatctg cgcaagcacg gcgtgtcggt aatgcgcgga ccgggccgtc
    17881 acacccctac gggcagcgaa ttcctttatt tcctcgaccc ggttggcttg accatcgagt
    17941 gtagtcacga tcgaaaccgc gcgcccgctt gcctggacga acgcatgtcc gcttcgtggt
    18001 gcatcacgag atcgctgcga aagatcgcgt tatggcagcc tttggccaag tgaggcttgg
    18061 gctcgggaac agggggcgac cgcgtcgcgg cgacggcgtt caaacatggc aggtcgccgg
    18121 cgctatcatg tgcggactgg agggaggaac agccggtggc tgtcacgcac ctcgggtcac
    18181 gagcgtggag tcgtcatgtg caggaacatc aagactttgt ttaacttcga tccccccgcg
    18241 acggacgagg aagttcatgc ggcgtcgctg caattcgtgc gcaagctgag cggattcaat
    18301 catccatcca ggacgaacga agcggcgttc gcccgcgcgg tcgatgacgt cgccgcggtt
    18361 gcgcggacgc tgatcaagtc cctcgtaacg tccgcggagc cgcgttaccg cgacgtcgaa
    18421 gccgagcgcg ttcgtgcaag aacgcttgcg cgcttcggtt cgcgcgggtg atacgcgggg
    18481 tgcgatgctt gagatcctct ggcgatcctc gtcgatcaac gcacggaagc tcgacttcga
    18541 aaccgcggaa agaagaaatg aaaccaattg ctgccgtatt cgctctgccg atcgtactcg
    18601 cgattacggg atgcgcactg attccgactt catcctcgag tccgcggacg acgcgttcgt
    18661 gcgtagtctc gggttgtggt gtcccagtcg agcaccatgg gtggtggccg ttctatggac
    18721 tcgaggtggc cgacgaagtc gatgtcaccg ctccgcgccc cacgatcatc acatggaaac
    18781 tgatcgggcg gccgcagacc cgctttcatc atacgaaagg gatcgtcttc gaggatgtcc
    18841 gcatcatctg caaggtcgac gaacaggacg atcacatcta cagctgcacg gacaatgcac
    18901 caccagggga atacaaatac tcgatccagg cgataggtcc tggcgcaccg ccaaacccta
    18961 aggacccctg gatcgtgaac cgctaggcag cggggaagcg ggccatagcg cagcgaggac
    19021 gtgccgcgga taggcgcatc tttttgcttg ctcgccacga cgcagcacca ttcactccgc
    19081 gaagcggccc acgacctttc tcttgagcga gatctgtcgc ccgcggatac tgcgccgaca
    19141 agcgctgcgt cgcagtcagt gccggcgcat ccttgaccgg ttggcgtagt cggtaaccca
    19201 ccgtcgaacg cggaacgcca agtggcccac aagctcgccg gtgcgacaga ccccgatctc
    19261 gaacgaagtg gatctgctcg cgacacgcct gtggctcact attttttcgg ttcgtctcgc
    19321 gcatgatgtc gacctcgggg tcgcattccg cgagcatgcc tcactccatg tggtttggcg
    19381 cagcgtgcgc acggaccgtg gcgtcccggt tacttgcctt ccgttcccgg caacggattg
    19441 agctgcatga cttcgatgga tgcctgcggg attgcgaagt gggggtgacc ctcgaaggct
    19501 cttgtcgccg catcgtgcga ttcggcttcg acgatggcat aaagcatgat gtcgtttctc
    19561 gcgtcggcga cgccttgtgc ggttatccgc ttggtcttgc ccacaccggc gcccttgtcg
    19621 gcgaacagct tggcgtgacc cgccatccag tttcgccaat cccgcatcat cttctcctcc
    19681 ggctccttgc gcttttcggc gggggtcttc ttccattcgt cgatgaccgc ggcgggcgca
    19741 atgtacaaga caaggaatct gctcatctcc tggctccttt cttcgctggg gcgatggccc
    19801 gcgctcactt ttcgcccggg cctgcaaagc gttcggcatg gtcatcgagg ttcgagttcc
    19861 tgggtcaaat ggcaggactc gcctcggtcg ttgcttcacc cgctgcgcat tcacgaatcg
    19921 catcgagcga ggcggtggct tgcgatccgt ggcagactga tttaccggtg cgccagtccg
    19981 gcaaccgcct actcgacaac gcgcgccaac gttcattgga attcccccag tttcccggac
    20041 acatccatta atgccaatat ggcaactgga ggtgtgcgat ggcagcaggg acacgacgga
    20101 agtttacggc ggaattcaag cgtgaggcag tgaaacttgc ggcgcagccg ggtcgttcgg
    20161 tgagtgcggt ggcgcgcgag gtgggagtcg aacgttcagt gctgaagcat tgggtcgaca
    20221 actttgcggc cgggaagtac gagaaggatc gcgcattgcc gctgaaggcc gtgcagcagc
    20281 aagaaaatga gcggttgcgc cgagagctgg cgaaggttcg aatggagcgc gacattctaa
    20341 aaaaagcgct gggctacttc gccaaggaac cgacgtgaag tttgcgtttg tagcccggta
    20401 tcgtccggta tggccgacgc gaatgatgtg tcggctgctc aacgtatcga ccagtggttt
    20461 ctacgagtgg cttggacggc caccgagcgc gaggtcagtg gcgaacgcga gcctgttggt
    20521 gcgcattcga gagagcttcg ccttgagtgg tcagacctac ggttcaccgc gagtatggaa
    20581 ggatatggtt gtggcgggtg aacgctgcag cgagaatcgc attgcgcggt tgatgcgagg
    20641 agcgctgctc agcggccagg cgaagccacg acgtaagccg accgatgcag ggatgcgtcc
    20701 cgaaaatagc atcgcgccca atgtgctgga acgcgatttc acggcctccg cacccaatcg
    20761 aaagtgggcg gcggacttca cgtatatctg gaccggcgaa ggctggttgt ttctggcagt
    20821 ggtcatcgat ttgttcagcc gtcgtgtcgt ggggtggtcg atgcaggcga cgatgaccgc
    20881 gcagctcgtg ctcgacgcac tggtgatggc cctgtggcga cgcggcaagc caacggaact
    20941 gctacatcac tccgaccaag gcagccaata cgctagcgaa gacttccagc gtctgctttc
    21001 tgctgaaggt attgcctgca gtatgagccg gcgcggtgac tgctgggaca actccgccat
    21061 cgagagcttc ttcgcttcgc tgaagaagga gcgcgtctac cgcacgatct accgaacacg
    21121 ggacgaaggc cgtgccgaca tttttgacta catcgaagcg ttctacaatt cccggcgcag
    21181 acattcgacg ctcgggcagg tcagccccac agagttcgaa cagagctatg cggggttaac
    21241 gtagtgtccg gagaaccagg ggaattccac
//
LOCUS       DASSVX010000174         6851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1456963, whole genome shotgun sequence.
ACCESSION   DASSVX010000174 DASSVX010000000
VERSION     DASSVX010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456963"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFO53_07525"
     CDS             complement(<1..326)
                     /locus_tag="VFO53_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012314739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET8876162.1"
                     /translation="MPPPDADRDEAFAAFALQAARDAGAAILPHFRAAIAIDDKGGAR
                     GYDPVTDADRAAEAAIRAAILARYPEHGVEGEEHGRHAGTSRYTWVIDPIDGTRSFIL
                     GHLHWAT"
     gene            complement(313..1245)
                     /locus_tag="VFO53_07530"
     CDS             complement(313..1245)
                     /locus_tag="VFO53_07530"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8876163.1"
                     /translation="MSKPRIGFVGVGLMGHGIAKNLLAKGFPLTLRVHRNRAAAQDLL
                     AAGAAEAASNAELARNADIVILCVTGAPQVEEVVLGPEGIASAAREGLIVVDTSTSEP
                     ATTSRMRNVLADQQVRFVDAPLARTPVEAEQGRLNIMIGADDATFAELQPTFAAFCEN
                     IVHAGPPGHGIVLKLINNFIAQAICTATAEACAAAAKSGLSIRKLHQVISAGAVNSGL
                     FQMLVGKMLESGDLSGLKFTLSNAAKDLRYYTHFTESMMIPSIVGEAVHQSLVTANAL
                     GFGDKYVPSLIEAQEKLAGVSIVPRDGRRDDAAA"
     gene            complement(1262..2146)
                     /locus_tag="VFO53_07535"
     CDS             complement(1262..2146)
                     /locus_tag="VFO53_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8876164.1"
                     /translation="MPTAAIVLISSSVLCFTILDAITKFMTQRYSVPLLVWARYGVQF
                     VAMLVWLGPSMRLGLVRTQRLRVQAFRGVLLLASSLFFVHALRSLPLAEATALNYSTP
                     IIVVLMGRLLLQERLTQARLAFVAAGVAGMLMIVQPGSDVFHGAALFGLGAAIFYACY
                     QITTRMLAGEDPRVLLFYPALMGTVIMSALAPGFDWPLSMPWTHVLLIVCGGLFGTLG
                     HFLFILAFRLAPASALTPFTYTQLVWAMLLGWFLYGDFPNGYRVAGMAVIAGSGLLMM
                     LHERRRARLVAIDPVTVQ"
     gene            2345..2797
                     /locus_tag="VFO53_07540"
     CDS             2345..2797
                     /locus_tag="VFO53_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET8876165.1"
                     /translation="MPVVSLARLLFVATLAMLAGGASLAAPDALAQAASTRQNRVVMQ
                     VSDNDPAKWNLALNNARNLQTDLGAANVDIEIVAYGPGIGMLKSESVVGNRITEALGQ
                     GVKVSACENTMRGQKLAKADMLDGIGYVPAGVVEIMQKQQQGWAYLRP"
     gene            complement(2825..2898)
                     /locus_tag="VFO53_07545"
     tRNA            complement(2825..2898)
                     /locus_tag="VFO53_07545"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2864..2866),aa:Gly,seq:ccc)
     gene            3101..4237
                     /locus_tag="VFO53_07550"
     CDS             3101..4237
                     /locus_tag="VFO53_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET8876166.1"
                     /translation="MSAPRRTLIAVVCLLAIAAVAAVAFRFAPGSRAALPKSAPAVPV
                     SVAPVVTKSVPVRLRAIGNVEPYTTVAVKARIDGQIVSVHFKEGQEVHKGETLFEIDR
                     RPFEAQLAQAQANLGKDRAVLDHANEQQKRYKELLEQKFVSPDAYAQIRTNAATAAAQ
                     LRADEAAIDSARLQIGYCTIRSPITGYAGKIQIQEGNLVKANDTNSLVVLNQVVPINV
                     SFSVPEQSLAAVRKYQADGELQVSAQVPSGGLAPVGGKLSFIDNTTDASTGTIKLKAE
                     FANTDKALWPGQFVDVVLTLTHQDNAIVVPATAVQNGPNGQYVFVVKPDRSVDLRDIK
                     VARNEGDIAVMASGLKPGETVVTVGQLRLAPGTKIAIADAKASE"
     gene            4268..>6851
                     /locus_tag="VFO53_07555"
     CDS             4268..>6851
                     /locus_tag="VFO53_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8876167.1"
                     /translation="MSGPQHTGGNIAALFVGRPVMTSLVMLGILIFGIVSYRLLPVNA
                     LPNVDFPTIQVSASLPGASPDTMASAVATPLEKQFSTIAGIDSMTSQSTQGSTSITIQ
                     FSLDRNIDAAAQDVQSAIAAAARSLPSAMPNPPTQRKVNPAESSILLLALTSKTQPLS
                     VVDDYAENNLAQRISTINGVAQVQVYGSQKYAVRIQLDPNALATRGIALTDVEQAVGN
                     ANVNIPTGTLYGAERATSVMATGQLNDAKEYAPMIVAYRNGAAVRLNQLGRVFDSVQN
                     DKVAAWYNGTRGVVLAISRQPGTNTIEVVDAIRAILPSFQAQLPPSVELSILYDRSES
                     IRESVHDVQYTLLLALGLVIGVIFVFLRSVTATIIPSLALPMSIIGTFSLMYLFGYSL
                     DNLSLMALTLCVGFVVDDAIVVLENVTRHMEMGKSRLQATLDGTKEIGFTIVSMTLSL
                     VAVFIPVLLMGGILGRLLHEFAVTIIVAVLLSGFVSLTLTPLLCSRMLKVERKEDHGR
                     IFAANERAFQAVLSAYRSSLQWVLHHRRLTMLVFTAIFVGTAFLFSWMPKGFLPSDDV
                     GQLFVITEAAQDISFDAMKNLQQQVAAIIQRNPHVENVMSFAGAGGPSSSLNNGRLFV
                     TLKPRNQRPTADEIVQELRPTLTRIPGINAYVQNVPTIRVGGRLSKSQYQYTVQAPDT
                     DELYKWAPQLETKIRTLPGLANVTSDLQITQPQITVDIDRNKASALGVSAQTIENTLY
                     DAYGARQVSTIFAPTDQYWVVMELEPRYQTDPSVLSLLYVRSSSGALVPLNAVATLRP
                     TVGPLAINHQGQLPAVTISFDTKPGVALSQAIEEINAATAQMQLPASVIGTFQGTAQA
                     FQSSL"
BASE COUNT         1226 a         2286 c         2280 g         1059 t
ORIGIN      
        1 gtcgcccaat gcagatggcc gaggatgaaa ctgcgcgtgc cgtcgatcgg gtcgatcacc
       61 cacgtgtagc gtgaggttcc cgcatggcgg ccgtgctcct cgccctcgac gccatgctcg
      121 ggatagcggg cgaggatcgc ggcgcgaatc gcggcttccg ccgcgcgatc ggcgtcggtc
      181 accggatcgt agccgcgcgc gccgcccttg tcgtcgatcg cgatcgctgc gcggaaatgc
      241 ggcaggatcg cagcgccggc atcgcgcgcg gcctgcagcg cgaacgccgc gaacgcttcg
      301 tcgcgatcgg cgtcaggcgg cggcatcgtc gcggcggccg tcgcgcggga cgatcgacac
      361 gccggcgagc ttctcctgcg cctcgatcag cgacggcacg tatttgtcgc cgaagccgag
      421 cgcgttcgcc gtgacgaggc tctgatgcac cgcctcgccg acgatcgacg gaatcatcat
      481 cgattcggtg aaatgcgtgt agtagcgcag atccttcgcg gcgttcgaca gcgtgaactt
      541 gagccccgac agatcgccgc tttcgagcat cttcccgacg agcatctgga agaggcccga
      601 attgaccgcg cccgccgaga tcacctggtg cagcttgcgg atcgacagtc ccgacttcgc
      661 ggcggcagcg caggcttccg cggtggcggt gcagatcgcc tgcgcgatga agttgttgat
      721 gagcttcagc acgatgccgt gcccgggagg tccggcatgg acgatgtttt cgcagaacgc
      781 cgcgaacgtc ggctgcaact cggcgaacgt cgcgtcgtcg gcgccgatca tgatgttgag
      841 ccgtccctgc tccgcctcga ccggggtgcg tgcgagcggc gcgtcgacga agcgcacctg
      901 ctgatcggcg aggacgttgc gcatccgcga cgtcgtcgcc ggctcgctcg tcgatgtgtc
      961 gacgacgatc agcccttcgc gcgccgcact cgcgatgccc tcgggaccga gcaccacttc
     1021 ctcgacctgc ggcgcgccgg tcacgcagag gatcacgatg tcggcgttgc gcgcaagctc
     1081 ggcgttgctt gccgcttctg ccgcgccggc ggcgagaagg tcctgcgcgg ccgcgcgatt
     1141 gcggtgcacc cgcagcgtca gcgggaagcc ttttgccagc aggttctttg caatgccatg
     1201 gcccatcagg ccgacaccga cgaagccgat ccgcggtttg ctcatccgga actcctccat
     1261 ttcattgaac ggtgacaggg tcgatcgcga cgagccgtgc gcgacgccgc tcgtgcaaca
     1321 tcatcagaag gccgctgccc gcgatcaccg ccattccggc gacacggtag ccgttcggaa
     1381 aatcgccgta gagaaaccag cccagcagca tcgcccagac gagctgcgta tacgtgaacg
     1441 gcgtcagcgc cgacgcgggt gcgaggcgga aggcgaggat gaacaggaaa tggccgagcg
     1501 tgccgaacag gccgccgcag acgatcagca gcacgtgcgt ccacggcatc gacagcggcc
     1561 agtcgaagcc aggtgccaac gccgacatga tgaccgtgcc catcagcgcc ggatagaaca
     1621 gcaggacgcg cgggtcctcg ccggcgagca tgcgcgtggt gatctggtag caggcgtaga
     1681 agatcgcggc gccgagtccg aacagcgcgg cgccgtggaa cacgtcgctg cccggctgga
     1741 cgatcatcag catgccggcc acgccggcgg cgacgaatgc aaggcgcgcc tgtgtcagcc
     1801 gctcctgaag gagcaggcgg cccatcagca cgacgatgat cggcgtcgag tagttgagcg
     1861 cggtcgcttc ggcgagcgga agcgagcgca gcgcatggac gaagaaaagc gacgatgcga
     1921 gcagcagcac gccgcgaaac gcctggacgc gcaagcgctg cgtgcgcacg agaccgagac
     1981 gcatgctggg cccgagccac acgagcatcg caacgaactg gacgccgtag cgggcccaga
     2041 cgagcagcgg caccgaatag cgctgcgtca tgaacttggt gatcgcgtcg agaatggtga
     2101 agcacagcac cgagctcgag atgaggacga tggcggcggt cggcacgtgg ccaccgaagg
     2161 gccgccccga cgtgcgaaca gcgccccccg ggggacagcg tagcggcgca aggggcgacc
     2221 gtggagaaac cttcatgcca ttgcgcatcg cctggattat cgccgatctc aatcgtgcag
     2281 ccgatccggc ataatcgtcg cttcggcggg cgccgcccgc ccgtccaccg cgccgaggtc
     2341 acccatgccg gtcgtttcgc ttgctcgact gctgtttgtc gcgacgctcg ccatgctggc
     2401 cggcggcgcc tcgcttgccg cgcccgatgc gctcgcgcaa gccgcgagca cccggcagaa
     2461 ccgcgtcgtg atgcaggtca gcgacaacga ccccgcgaaa tggaacctcg ccctcaacaa
     2521 tgcccggaac ctgcaaaccg acctcggcgc ggcgaacgtc gacatcgaga tcgtcgcgta
     2581 cggccccggc atcggcatgc tcaagtcgga atcggtcgtc gggaaccgca tcaccgaggc
     2641 gctcggtcag ggtgtgaagg tcagcgcttg cgaaaacacg atgcgcggtc agaagctcgc
     2701 gaaagccgac atgctggacg gcatcggcta cgttcccgcc ggggtcgtcg agatcatgca
     2761 gaagcagcag cagggctggg cgtatctgcg gccgtaaggc cacggcgcgc gcgtgcgagg
     2821 cggatggagc gggtgaaggg aatcgaaccc tcgtatgcag cttgggaagc tgccgttctg
     2881 ccattgaact acacccgcgc cgggttgcgc ataagcggtt cgacgtcgcg taaaacgatc
     2941 ttcgcggtcg gctgcgattg tagcgccgcg cgggcgtcgc gccacatgtt tccgaatgtg
     3001 tcgggcgcgt gggcgggtgg cggacggctt aaactcgaga gtttgaatgc gtcgcccgat
     3061 gccgcgggaa tcaacgctcc gcctccatgg tcctagcacg atgtccgcac cgcgccgcac
     3121 actgatcgcc gtcgtctgcc tgctggcgat cgcagccgtc gcggcggtcg cattccggtt
     3181 tgcaccgggt tcgcgcgccg cactgccgaa gtcggcgccg gcggttccgg tgagcgttgc
     3241 gcccgtcgtc acgaagagcg ttcccgtgcg actgcgcgcc atcggcaacg tcgagccgta
     3301 caccaccgtc gcagtgaagg cgcgcatcga cggccagatc gtatcggtgc atttcaagga
     3361 agggcaggaa gtgcacaagg gcgagacgct gttcgagatc gaccggcggc cgttcgaagc
     3421 gcagctcgca caggcgcagg cgaacctcgg caaggatcgc gcggttctcg accacgcgaa
     3481 cgagcagcag aagcgctaca aggagctgct cgagcagaag tttgtgtcgc ccgacgcgta
     3541 tgcacagatt cgcacgaacg cggccaccgc cgccgcgcag ctacgcgccg acgaagcggc
     3601 gatcgacagc gcacggctgc agatcggcta ctgcacgatc cgctcgccga tcaccggtta
     3661 cgccggcaag atccagatcc aggaaggcaa tctcgtcaag gcgaacgata cgaactcgct
     3721 cgtcgtgctg aaccaggtcg tgccgatcaa cgtgtcgttc tccgtgcccg agcagagcct
     3781 tgccgcggtg cgcaagtacc aggccgacgg cgagcttcag gtgagcgcgc aggtgccgag
     3841 cggtggcctt gcgccggtgg gcggcaagct ttcgttcatc gacaacacga ccgacgcgtc
     3901 gaccggaacc atcaagctca aggccgaatt cgcgaacacc gacaaggcgc tgtggccggg
     3961 gcagttcgtc gatgtcgtgc tcacgctcac gcaccaggac aacgcaatcg tcgttccggc
     4021 caccgccgtg cagaacggtc cgaacggcca gtacgtgttc gtcgtcaagc ccgaccgcag
     4081 cgtcgacctg cgcgacatca aggtggcgcg aaacgagggg gatatcgccg tgatggcctc
     4141 ggggctcaag ccgggcgaga ccgtcgtcac cgtcggtcag ctgcgcctcg cgccgggaac
     4201 gaagatcgcg atcgccgacg cgaaggccag cgagtgaccg gcagcacgca gcggcggcgc
     4261 tgacccgatg agcggaccgc agcacaccgg cggcaacata gcggcgctgt tcgtcggccg
     4321 gccggtcatg acgtcgctcg tcatgctcgg catcctgatc ttcggcatcg tcagttaccg
     4381 gctgctgccg gtgaatgcgc tgccgaacgt cgactttccg acgatccagg tatcggcaag
     4441 cctgccgggg gcgagtcccg acacgatggc gtccgccgtc gccacgccgc tcgaaaagca
     4501 gttctcgacg atcgccggca tcgactcgat gacgtcgcag tcgacgcagg gcagcacgtc
     4561 gatcaccatc cagttctcgc tcgatcgcaa catcgacgcc gccgcccagg acgtgcagtc
     4621 ggccatcgcc gccgcggcgc gctcgctgcc gtcggcgatg cccaacccgc cgacgcagcg
     4681 caaggtcaac ccggccgaat cgtcgatcct gctgctggcg ctcacgtcga agacgcagcc
     4741 gctgtcggtg gtcgacgact acgcggagaa caacctcgca cagcgcatct cgacgatcaa
     4801 cggcgtcgcg caggtgcagg tctacgggtc gcagaagtac gcggtacgca tccagctcga
     4861 tccgaacgcg ctcgccacgc gcggcatcgc gctgaccgac gtcgagcagg cggtgggcaa
     4921 cgcgaacgtc aacatcccga ccggcacgct gtacggcgcc gagcgcgcga catcggtgat
     4981 ggcgacgggg cagctcaacg acgcaaagga atacgcgccg atgatcgtcg cctaccgcaa
     5041 cggcgccgcc gtgcggctga accagctcgg gcgcgtgttc gacagcgtgc agaacgacaa
     5101 ggtggcggcc tggtacaacg gcacgcgcgg cgtcgtgctc gccatcagcc gccagccggg
     5161 caccaacacg atcgaggtcg tggacgccat ccgcgcgatc ctgccgtcgt tccaggcgca
     5221 gcttccgccg tcggtcgaac tgtcgattct ctacgatcgc tccgaatcga tccgcgagtc
     5281 ggtgcacgac gtgcagtaca cgctgctgct cgcgctcggt ctcgtcatcg gcgtgatctt
     5341 cgtgttcctg cgcagcgtca ccgcgacgat catcccgagc ctcgcactgc cgatgtcgat
     5401 catcggcacg ttctcgctga tgtacctgtt cggctacagc ctcgacaacc tgtcgctgat
     5461 ggcgctgacg ctctgcgtcg gcttcgtcgt cgacgacgcg atcgtcgtgc tcgagaacgt
     5521 cacgcggcac atggagatgg ggaagtcgcg ccttcaggcg acgctcgacg gcacgaagga
     5581 gatcggcttc acgatcgtgt cgatgacgct gtcgctcgtg gcggtgttca ttccggtgct
     5641 gctgatgggc ggcatcctcg ggcgtctgtt gcacgaattc gccgtgacga tcattgtcgc
     5701 cgtgctgctg tcgggcttcg tgtcgctgac gctgacgccg cttctgtgca gccgcatgct
     5761 gaaggtcgag cgcaaggagg accacgggcg catcttcgcg gcgaacgagc gtgcgttcca
     5821 ggcggtgttg agcgcgtacc ggagcagcct gcaatgggtg ctgcatcacc ggcgcctgac
     5881 gatgctcgtg ttcaccgcga tcttcgtcgg cacggcgttc ctgttctcgt ggatgccgaa
     5941 gggcttcctg ccgagcgacg acgtgggcca gctcttcgtc atcacggagg cggcgcagga
     6001 catctcgttc gacgcgatga agaacctgca gcagcaggtg gccgccatca tccagcgcaa
     6061 tccgcacgtc gagaacgtga tgtcgtttgc cggcgccggc ggcccgagct cgtcgctgaa
     6121 caacgggcgc ctgttcgtga cgctgaagcc gcgcaaccag cggcccaccg ccgatgaaat
     6181 cgtgcaggag ttgcggccga cgctgacacg catccccggc atcaacgcat atgtccagaa
     6241 cgtgccgacc attcgcgtcg gcggacggct gtccaagagc cagtaccagt acaccgtgca
     6301 ggcgccggac accgacgagc tgtacaagtg ggcgccgcag ctcgagacga agatccgcac
     6361 gctgcccggg ctcgccaacg tgacgagcga cctgcagatc acgcaaccgc agatcaccgt
     6421 cgacatcgac cgcaacaagg cgtcggcgct cggcgtgtcg gcgcaaacga tcgagaacac
     6481 gctgtacgac gcctacggcg cgcggcaggt gtcgacgatc tttgcaccca ccgaccagta
     6541 ctgggtcgtg atggagctcg agccacgcta ccagaccgat ccgtccgtgc tgtcgctgct
     6601 gtacgtgcgc tcgagcagcg gggccctcgt tccgctcaac gccgtcgcca cactgcggcc
     6661 gacggtcggc ccgctcgcga tcaaccacca gggccagctg ccagccgtga cgatctcgtt
     6721 cgacacgaag ccgggcgtgg cgctgtcgca ggcgatcgag gagatcaatg cggcgaccgc
     6781 gcagatgcag ctgccggcct cggtgatcgg cacgttccag ggaacggcgc aggcgttcca
     6841 atcgtcgctg c
//
LOCUS       DASSVX010000175         4239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_573758, whole genome shotgun sequence.
ACCESSION   DASSVX010000175 DASSVX010000000
VERSION     DASSVX010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573758"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VFO53_07560"
     CDS             <1..931
                     /locus_tag="VFO53_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET8876168.1"
                     /translation="PYQYRQFRIIEFPRYAAFAQSFPNTVPYSEGIGFIARVRDADPE
                     DIDYPYYVTAHELAHQWWGHQVPGANVQGDTMLVETLAQYSALMVMKKKYGHAKMQRF
                     LRYELDRYLIGRSTEQKKELPLAQVENQDYIHYRKGSLVMYALADYIGEENLNRAIRA
                     YRDKWAFKGPPYSTTKDLLAEIRAVTPPALAYVIDDLFDTITLYDNRALSATAKALPD
                     GRYEVTLKVFARKLKADELGKETEVPLADFIDIGVEDEHSEPLFLEKRRIAAEESTIS
                     VIVARKPARAGIDPYNKLIDRRPKDNTLPVTVE"
     gene            complement(957..1442)
                     /locus_tag="VFO53_07565"
     CDS             complement(957..1442)
                     /locus_tag="VFO53_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876169.1"
                     /translation="MSNAISVVIGLVLAVASATVLAQPAENADQAAHKLNPYVYMRNA
                     AGDKVFLETQFWRNDPNYDARALRRVSDVMAGLEKLGFKGNPDAANGWDKPEKVVRCR
                     INMEVVNKRWRGKTGAIVACENTGISDAEVVPSEDPKHVSLVLQAFDKQYKLAKEKQG
                     K"
     gene            complement(1530..2111)
                     /locus_tag="VFO53_07570"
     CDS             complement(1530..2111)
                     /locus_tag="VFO53_07570"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HET8876170.1"
                     /translation="MATAQPASSANPHGHRRGWSIAWGILLIVIGILALLMPEVAALA
                     TVLTLAWLLIFAGVVELVHAFQTRRRSGFGWKLAAAIITLILGLCILLFPVAGIATLA
                     LWIGAFIFAGGIVRLILAFRVRPAKGWGWVLFDGLLAIVIGGLIAWGWPASSIGFIGL
                     LTGFWLLFSGVWRIMLDGRAPQPGEGGVLSHAA"
     gene            complement(2131..3078)
                     /locus_tag="VFO53_07575"
     CDS             complement(2131..3078)
                     /locus_tag="VFO53_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HET8876171.1"
                     /translation="MTSGFARPLPQAATARAVCDRLPRAALIGNPDRPIRAIGALSTH
                     ESDVLAFCDAARAAEHLAHTPAAVVIVPNVLATMPRAEQTFVAVDDVRSAFIDIVEAL
                     LPHSGRPVDPLPGIDAQAAIGEGAAISPLACVGPRVRIGAGTRVGPGAVLYEDSEIGR
                     DCVIGPGAIIGWLGLAYHDRADGRRSFFPHLAGVRIGDRVDVGAHACICRGMLSHTTI
                     ASDAKLGSLVYVSHGVDIAARAWLSAGTAIAGHATIAANALLGIGSVVVDNVDVGADV
                     MVGGGSVVIKDAGAGSRLHGVPAQPVAAMRRFGPTPRER"
     gene            complement(3075..3905)
                     /locus_tag="VFO53_07580"
     CDS             complement(3075..3905)
                     /locus_tag="VFO53_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876172.1"
                     /translation="MGTDLQTPFDASVVMVTVVRDTFAQALRSVFAQAFAGRIQVLVG
                     VDRWIGDRAAVEDLARQSPSHIGVTILDLGYSTSARNGGLYPSRYGGALKTILSYAAN
                     SRYVTYLDDDNWYAPEHIASMVAAVTGKAWAFSLRHFVEARTSTLLCRDTWESIGPGR
                     GVYAQAQGGFVDTNCFFIDKLACHDVFPEWAMTRFAGGTGGDRQVLERLRGRPFGTND
                     AHTVFYRTPLAGLHPYLLWRFKCAGVDLARYLPHDGLPGEAVWRQCAEHDARTRVELA
                     "
BASE COUNT          761 a         1401 c         1432 g          645 t
ORIGIN      
        1 cccgtatcag tatcggcagt tccgcatcat cgagttcccg cgctacgcgg cgttcgcgca
       61 atcgtttccg aacacggtgc cctactcgga gggcatcggc ttcatcgccc gcgttcgcga
      121 cgccgatccg gaggacatcg attacccgta ttacgtcacg gcgcacgagc tcgcccacca
      181 atggtggggc caccaggtgc cgggtgcgaa cgtgcagggc gacacgatgc tcgtcgagac
      241 gctcgcgcag tattcggcgc tgatggtcat gaagaagaag tacggccacg cgaagatgca
      301 gcggttcctg cgctacgagc tcgaccggta cctgatcggc cgcagcaccg agcagaagaa
      361 ggagctgccg ctcgcgcagg tggagaacca ggattacatc cactatcgca agggcagcct
      421 cgtcatgtat gcgctcgccg actacatcgg cgaggagaat ctgaatcgcg ccatacgcgc
      481 gtatcgcgac aagtgggcgt tcaaggggcc gccgtactcg acgacgaagg acctgctcgc
      541 ggagatccgc gcggtgacgc cgcccgcgct cgcgtacgtc atcgacgatc tattcgacac
      601 gatcacgctc tacgacaatc gcgcgttgtc ggcgacggcg aaggcgcttc ccgacgggcg
      661 ctacgaggtg acgctcaagg tgtttgcgcg caagctcaag gccgacgagc tcggcaagga
      721 aaccgaggtg ccgctcgccg atttcatcga catcggcgtc gaggacgagc acagcgagcc
      781 gctcttcctc gaaaagcggc ggatcgcggc agaggagtcg acgatcagcg tgatcgtcgc
      841 gcggaagccg gcgcgggccg gcatcgatcc ctacaacaag ctgatcgatc ggcggccgaa
      901 ggacaacacg ctgccggtca cggtcgaata gcgaaagcct ccgccccggc gatcgcctac
      961 ttgccctgct tttccttggc gagcttgtac tgcttgtcga acgcctgcag cacgaggctc
     1021 acgtgcttcg gatcttccga cggcaccacc tcggcgtcgg agatgccggt gttctcgcag
     1081 gcgacgatgg cgccggtctt gccgcgccag cgcttgttga cgacttccat gttgatgcgg
     1141 cagcgaacca ctttctcggg cttgtcccag ccgttggcgg cgtcgggatt gcccttgaag
     1201 ccgagcttct cgaggccggc catcacgtcc gacacgcgtc gcagcgcgcg cgcatcgtaa
     1261 ttgggatcgt tgcgccagaa ctgggtctcg agaaagacct tgtcgccggc ggcattgcgc
     1321 atgtagacgt acgggttgag cttgtgcgcg gcctggtcgg cattttcggc cggctgcgca
     1381 aggacggtcg cgctggccac tgccagcacg agaccgatca caacggagat cgcgttcgac
     1441 attcgggtca ggcctcgctt gaatgaacgg tcatttctag catatccgcg tttcgaatgc
     1501 gcaaaatgcc ctctccaggc aggcgcaggt cacgctgcgt gcgacagcac gccgccttct
     1561 ccgggctgcg gggcacgtcc gtcgagcatg atccgccaga caccggagaa cagcagccag
     1621 aagccggtta gcagtccgat gaagccgatc gagcttgccg gccaccccca ggcgatcaac
     1681 ccgccgatca cgatcgcgag caggccgtcg aagaggaccc acccccagcc cttcgccggc
     1741 cgcacgcgga acgcgaggat caggcgcacg atgccgccgg cgaagatgaa cgcgccgatc
     1801 cagagcgcga gcgtcgcgat gccggccacc gggaagagga ggatgcagag tccgaggatc
     1861 agcgtgatga tggctgccgc gagcttccag ccgaaaccgc tgcggcgccg ggtctggaag
     1921 gcgtggacga gctcgacgac gccggcgaag atcagcaacc acgcgagcgt caggacggtc
     1981 gcgagcgctg cgacttccgg catcagcagc gcgagtatgc cgatgacgat cagcaggatt
     2041 ccccaagcga tcgaccagcc gcggcggtga ccgtgcgggt tcgcactcga agccggttgc
     2101 gccgttgcca tcgttgcctc ctgttgcggt tcaccgctcg cgcggcgtgg gcccgaagcg
     2161 gcgcatggca gccaccggtt gcgccggtac gccgtgcagc ctcgagcctg cgcccgcatc
     2221 cttgatcacg acgctgccgc cgccgaccat cacgtcggcg ccgacatcga cattgtcgac
     2281 gacgacgctt ccgatgccga gcagcgcatt cgcggcgatc gttgcatgtc ctgcaattgc
     2341 cgttccggcc gagagccagg cccgcgcagc gatgtcgacg ccgtggctca cgtagacgag
     2401 gctgccgagc tttgcgtcgg aggcgatcgt ggtgtgcgag agcatgccgc gacagatgca
     2461 ggcgtgggcg cccacgtcga cgcggtcgcc gatacgcacg ccggcaagat gcggaaagaa
     2521 gctgcgccgg ccatcggcgc ggtcgtggta ggcaagcccc agccagccga tgatcgcgcc
     2581 cggaccgatc acgcagtcgc ggccgatctc gctgtcctcg tagagcacgg cacccgggcc
     2641 gacgcgcgtg cccgcgccga tacgaacccg gggtccgacg cacgcgagcg gcgagatcgc
     2701 cgcgccttcg ccgatggccg cttgggcatc gatgccgggg agggggtcga cggggcgccc
     2761 ggagtgcggg agaagcgctt cgacgatgtc gatgaacgca ctgcggacgt cgtcgaccgc
     2821 gacgaacgtc tgctcggccc gcggcattgt cgcgaggacg ttcggcacga tgacgaccgc
     2881 ggccggcgtg tgcgcaagat gctcggcggc acgcgccgcg tcgcagaacg cgagcacgtc
     2941 cgattcgtgt gtcgacaatg caccgattgc acgaatcggc cggtcaggat tgccgatcag
     3001 cgctgcccgc ggcaggcggt cgcacaccgc gcgcgccgtc gcggcctgcg gaagcggacg
     3061 ggcaaacccg gaggtcatgc caactcgacc cgcgtacgcg catcgtgctc ggcgcattga
     3121 cgccataccg cctcgccggg gagcccatcg tgcggcaggt aacgggcgag gtccacgccc
     3181 gcacacttga agcgccacag caggtacgga tgcagtccgg cgagcggcgt gcgatagaac
     3241 accgtgtgcg cgtcgttggt gccgaacggg cgcccgcgca gcctttccag cacttggcga
     3301 tcgccgccgg tgcccccggc gaaccgcgtc atcgcccatt cggggaatac gtcgtggcag
     3361 gcgagcttgt cgatgaaaaa gcagttcgtg tcgacgaacc cgccctgcgc ctgcgcgtag
     3421 acgccccgtc cgggcccgat cgactcccac gtatcgcggc agagcagcgt gctggtccgg
     3481 gcctcgacga agtggcgaag cgaaaacgcc cacgccttgc cggtgactgc cgccaccatc
     3541 gaggcgatgt gttcgggcgc gtaccagttg tcgtcgtcga gatacgtgac gtagcggctg
     3601 ttcgcggcat agctcaggat cgtcttcagc gcgccgccgt agcggctcgg gtacaggcca
     3661 ccgttgcgcg ccgatgtcga gtagccgaga tcgaggatcg tgacgccgat gtgcgacgga
     3721 ctctggcgcg cgagatcctc gacggccgca cggtcgccga tccagcggtc gacgccgacg
     3781 agcacctgga tgcggccggc gaacgcctgc gcgaagacgg agcgcagcgc ctgcgcaaag
     3841 gtgtcgcgga cgacggtgac catgacgacg ctcgcgtcga agggagtctg gaggtcggtg
     3901 cccatcgccc ggggtgtcca ttgaaccgca gcgaatcata cccaaagcac gggaaccgcc
     3961 cggcgcggcc gcctgcccaa gcacacttgg ccgccgcacg cgacgagccg cgaatcggcc
     4021 cgctgtctcc gtgggcaaat gcgtggcatg gaagaggctg atggttgacg agaaggacgc
     4081 gatgacccta taatcgcggt cttttcgcct cccgaacaag gccagcaaac gctgccttcc
     4141 gggggccaag gcgtctcggc actgcggcat cggtgcaaag ctgcggcgca aaaagtggcc
     4201 taagccggcg cttcgcgact gtaatcgcgg agcgggatg
//
LOCUS       DASSVX010000176         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_540242, whole genome shotgun sequence.
ACCESSION   DASSVX010000176 DASSVX010000000
VERSION     DASSVX010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540242"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFO53_07585"
     CDS             <1..430
                     /locus_tag="VFO53_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET8876173.1"
                     /translation="SALLGTYLSEVQAPQRLAQVVADITRNKWLVLALLNVLFLLLGM
                     FLHSAAAIILVVPVVMPLVRAVGIDPVHFGLIVTVNLGIGQQTPPVASVLMVASAIAR
                     ESIWAVTRINIYFIAVLVAVLLLVTYVPVTGLGLVELFYR"
     gene            427..1221
                     /locus_tag="VFO53_07590"
     CDS             427..1221
                     /locus_tag="VFO53_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET8876174.1"
                     /translation="MSESARDATVITSRDGAIATLTLNRPHKLNALTLSMWKALGDAI
                     TELSADDQLRCVVLRGAGERAFSPGTDIGEFAHERANSTQARTYGAVMHRTLRALTDC
                     PIPLVAQIHGICVGGGLEIAATCDLRICGASSRFGAPVKNLGLVMAHAEMAALVRLVG
                     TAVGLEILLEGRIFDAAEAQAKGLVTRVVADADVVSEAAATAARIAEGAPLVARWHKR
                     FARRLADSTPLSAAEADEGFACFDTDDFRIGYAAFLAKRKPEFTGK"
     gene            1291..>2103
                     /locus_tag="VFO53_07595"
     CDS             1291..>2103
                     /locus_tag="VFO53_07595"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8876175.1"
                     /translation="MRVLELAQIMAGPTCGMLLADMGADVVKVEKLPGGDDSRSYSEP
                     RINGESAPFMMLNRNKRGIAIDLKKPAGRDVLLRMVRDADVLIENYRRGTLEKLELGY
                     DVLSKTNPGLIYCAISGYGRDGPFGDKGGFDLIAQGFAGLMAITGEPGGPPVKTGPPV
                     ADINAGILAAVGILAAYAHKQRTGQGQVVDTSLMEAALQQTYWHAAIYFATGASPGPT
                     GSAHLLTAPYQAFAASDGWLNIGGANQANWERIAAMLGHPEWCDDPRFISNAA"
BASE COUNT          337 a          670 c          715 g          381 t
ORIGIN      
        1 gagcgcgctg ctcggcacct atttgagcga agtgcaggcg ccgcagcggc tcgcgcaggt
       61 tgtcgcggac atcacgcgca acaagtggct cgtgctggcg ctgctcaacg tgctgttcct
      121 gttgctcggc atgttcctgc actcggccgc ggcgatcatt ctggtcgtgc ctgttgtgat
      181 gcccctcgtt cgcgccgtgg gcatcgaccc cgtgcatttc ggcctgatcg tcaccgtcaa
      241 tctcggcatc ggtcagcaga cgccgccggt tgcgagcgtg ctgatggtcg cgagcgcgat
      301 cgcccgcgag tcgatctggg cggtgacgcg gatcaacatc tatttcatcg ccgtgctcgt
      361 tgccgtgctg ttgctcgtta cgtacgtgcc ggtgacgggg ctcggtctcg tcgagctttt
      421 ctaccgatga gcgaatccgc gcgcgacgct acggttatca cgtcccgcga cggcgccatc
      481 gcgacgctga cgctcaaccg gccgcacaag ctcaacgcgc tgacgctgtc gatgtggaag
      541 gcgctcggcg atgcgattac cgagctgtcc gccgacgatc aactgcgctg cgtcgtcctt
      601 cgcggcgccg gcgagcgtgc attctcgccc ggcaccgaca tcggcgagtt cgcgcacgag
      661 cgcgcgaatt cgacgcaggc gcgcacttat ggcgctgtga tgcacaggac actgcgtgcg
      721 cttaccgatt gtccaatccc gctcgtcgct caaatccacg gtatctgtgt tggcggtggt
      781 ctcgagatcg ctgcgacgtg cgacctgcgg atctgcggcg cgtcgagtcg cttcggcgca
      841 ccggtgaaga atctcgggct cgtcatggcg cacgccgaga tggccgcact ggtgcggctc
      901 gtgggtaccg ccgtcgggct cgagatcctg ctcgagggac gcatcttcga cgcggctgaa
      961 gcccaggcga agggactcgt aacgcgtgtt gttgccgacg cggatgtcgt tagcgaagcg
     1021 gcggcgacgg cggcgcggat tgccgaaggc gcgccgctcg tcgcgcgctg gcacaagcgg
     1081 ttcgcgcgtc ggctcgcgga ttcgacgccg ttatctgccg cggaagccga cgagggcttt
     1141 gcgtgcttcg acaccgacga ctttcgcatc ggctacgcgg cgtttctcgc gaagcgcaag
     1201 cccgagttca cgggtaaatg acggtggtgc agcaacgtgg ctgaggcggc gaagaacgcg
     1261 atcgcaacgc atccggggcc gctgcgcggc atgcgcgtgc tcgagcttgc gcagatcatg
     1321 gccggcccga cctgcggcat gctgctcgcc gacatgggcg cggacgtcgt caaggtcgag
     1381 aaacttcccg gcggcgacga ttcacgcagc tacagcgaac cacgcatcaa cggcgagtcg
     1441 gctcccttca tgatgctgaa tcgcaacaag cgcggcatcg cgatcgatct caagaagccg
     1501 gccggccgcg acgtgctgct gcggatggtg cgggacgccg atgtgctgat cgagaactat
     1561 cggcgcggaa cgctcgagaa gctcgagctt ggctacgacg tgctttcgaa gaccaacccg
     1621 ggtctcatct attgcgcgat ctcgggctat ggccgtgatg gcccgttcgg cgacaagggc
     1681 ggcttcgacc tgatcgcgca agggtttgcc gggctgatgg cgatcaccgg cgagcccggg
     1741 ggaccgccgg tcaagaccgg tccgccagtt gcggacatca acgcgggcat cctcgccgcg
     1801 gtcggcattc tcgccgctta cgcgcacaag caacgcaccg ggcagggcca ggtcgtcgac
     1861 acgtcactga tggaagcggc cttgcagcaa acctactggc acgcggcgat ctatttcgcg
     1921 acgggcgcgt cgccgggacc gacaggatcg gcgcatctgt tgacggcgcc ctaccaggcg
     1981 ttcgccgcca gtgatggatg gctgaacatt ggcggcgcga accaggcgaa ctgggagcgg
     2041 atcgccgcga tgctgggcca tccggaatgg tgtgacgacc cgcggttcat ttccaatgcg
     2101 gca
//
LOCUS       DASSVX010000177         5929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1289642, whole genome shotgun sequence.
ACCESSION   DASSVX010000177 DASSVX010000000
VERSION     DASSVX010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289642"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFO53_07600"
     CDS             complement(<1..381)
                     /locus_tag="VFO53_07600"
                     /inference="COORDINATES: protein motif:HMM:NF022457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HET8876176.1"
                     /translation="MTPIVDIDVARWRPEVSPADTERHARELESGGVLALPHLAFELS
                     PAEQRFLDIRWSDGRAKNISLDGDAIKGAQGGAGDLDALAAMIARFAADASALIAALF
                     PRYAPYLARARTSYRPHGAAGRAVS"
     gene            complement(505..1941)
                     /locus_tag="VFO53_07605"
     CDS             complement(505..1941)
                     /locus_tag="VFO53_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HET8876177.1"
                     /translation="MGKITGFLEWQRIAEVSDPVRSRVRHFREFIHALDDAAASTQGG
                     RCMDCGIPFCQTGCPVNNIIPDWNDLVYRHQWKAALDVLHSTNNFPEFTGRICPAPCE
                     AACTLNINDDPVGIKSIEHFIIDKGFDEGWVVPLPPVQKTGRRVAVVGSGPAGLACAQ
                     QLARAGHDVVLFEKEDRIGGLLRYGIPDFKMEKHLIDRRMAQMSIEGVEFHPNSHVGG
                     NVSAQALLGEFDAIALSGGAEKPRDLPVPGRALRGVHFAMEFLPQQNRLVAGDAVTAQ
                     ISAAGKRVIVVGGGDTGSDCVGTSTRQGALSVTQFELLTQPPITENKPLVWPYWPIKL
                     RTSSSQEEGCERDWSVATKRFEGTNGQVEKLVAARVEWQRDGSGAMRMVEIPGSEFEM
                     KADLVLLAMGFTGPGETGLVEQFGVERDARSNVKATTDDYRTSVPKVFAAGDMRRGQS
                     LVVWAIREGRQCAQAIDAFLMGSSSLPR"
     gene            complement(1954..>5929)
                     /locus_tag="VFO53_07610"
     CDS             complement(1954..>5929)
                     /locus_tag="VFO53_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HET8876178.1"
                     /translation="NFNWIRAREGAIASKVLGDDLQKLWPLIYDGQSDSASYDNCLEL
                     LVMGGYSPAHAMMLMIPEAWAGNPLMDEDRRAFYEYHAALMEPWDGPAAMAFTDGRQI
                     GATLDRNGLRPARYIVTDDDFIVMSSESGVLDIPESKVVRKWRLQPGKMLLVDLDAGR
                     IVDDDELKRTLATAKPYRDWIARYRLRLEEMPEPAPTTPTSVPLLDRQQAFGYTQEDQ
                     KIILAPMAANAEEPVGSMGNDAALPVLSDRPKVFYNYFKQLFAQVTNPPIDPIREELV
                     MSLVSFIGPRPNLLALDPRDDDAEIANGVHGHGTTPDGEPPMRLEAQQPVLTPENMEK
                     LRHVELFSGGAFRSIELDICYPSAWGGNGMEAALASLAAHAEDAIHQGYNILILSDRM
                     IGADMLPIPALLATAAVHEHLVKKGLRTSTGLVVETGSAREVHHFALLAGYGAEAIHP
                     YLALETLAELSADAGKEAQKRYIKAICKGLCKVMSKMGISTYQSYCGAQIFEAVGLQK
                     AFVDKYFTGTASTIEGIGLFEVAAEAERLHALAFGNDPLLAGALDAGGDYMYRIRGEE
                     HMWTPDTIAKLQHAARANSHSTYKEYATLINEQGRRLKTLRGLFEFKTAGRTPVPIDE
                     VEPASVLVKRFATGAMSLGSISTEAHTTLAIAMNRIGGKSNTGEGGEDPMRYRAEMRA
                     GKSVVEDGDTLATIIGKGPVEHDIALKAGDSLRSRIKQVASARFGVTAEYLASADQLQ
                     IKMAQGAKPGEGGQLPGHKVSEYIAQLRFSVPGVGLISPPPHHDIYSIEDLAQLIHDL
                     KNANPRASISVKLVSEVGVGTVAAGVAKAKADHVTIAGHDGGTGASPESSIKHAGTPW
                     ELGLAETQQTLVLNRLRGRIAVQVDGQMKTGSDVAIGALLGADEFGFATAPLVVEGCI
                     MMRKCHLNTCPVGVATQDPVLRRRFTGQPEHVVNYFFFVAEELRAIMAGLGFRTLSEM
                     IGRSDLLDTKKGIEHWKAKGLDFSRVFHQPPMPPEVARHHCETQDHGLARALDHQLIE
                     AAAPAIDKGQPVRLASRIRNANRSVGAMLSGVVARRHGHDGLAEDTLHVAFEGIAGQS
                     FGAFLARGITFELQGGTNDYVGKGLSGGRIVVYPDPACPAKAEENIVIGNTVMYGAIA
                     GEAYFRGIAGERFCVRNSGASAVVEGTGDHGCEYMTGGTVVVLGRTGRNFAAGMSGGV
                     AYVFDADGSFASRCNPAMVALGPVLSESEQQRVEKELVSAGKGRLLHEGVADEAILRR
                     LIERHLRFTGSTLALSMLDRWDATRAKFVKVFPNEYVRALSEMHVKAQHAKPAGVPAQ
                     RAVA"
BASE COUNT          940 a         2018 c         1926 g         1045 t
ORIGIN      
        1 cgacacggcg cggcccgcgg cgccatgcgg ccgatagctc gtgcgcgccc gcgcgagata
       61 gggtgcgtag cgcgggaaga gcgctgcgat cagcgcactc gcatcggcgg cgaagcgcgc
      121 gatcatcgcc gcgagcgcgt cgagatcgcc cgcgccgccc tgcgcgccct tgatcgcatc
      181 accgtcgaga ctgatgttct tcgccctgcc atccgaccag cggatgtcga ggaagcgctg
      241 ctcggcgggc gacaattcga acgcgagatg cggcagcgcg agcacgccgc cgctctcgag
      301 ctcgcgcgca tggcgctcgg tgtccgcggg cgaaacttcg ggccgccatc gcgcgacgtc
      361 gatgtcgaca atcggggtca tggaaattgg ggccaggctc gattttccgg atgcactcag
      421 cgatcctgtg gtggcgcacc acggaaaatc gagcctggcc ccaatttccg tgcaccatgg
      481 aaaagcgacc ctggccccaa gcgcttacct cggcagcgac gacgatccca tcaggaacgc
      541 atcgattgcc tgtgcgcatt ggcggccttc gcgaatcgcc cacacgacaa gcgactggcc
      601 gcggcgcatg tcgccggcgg cgaagacctt cggcacgctg gtccggtaat cgtcggtcgt
      661 cgccttcacg ttgctgcgcg catcgcgctc gacgccgaac tgctcgacga gcccggtttc
      721 gcccgggccg gtgaagccca tcgcgagcag cacgagatcg gccttcatct cgaattcgct
      781 gcccggaatt tccaccatcc gcatcgcgcc gctgccgtcg cgctgccatt cgacgcgcgc
      841 ggcaaccagc ttctcgacct ggccattggt gccttcgaag cgcttcgtcg ccaccgacca
      901 gtcgcgctcg caaccttctt cctgcgacga cgacgtgcgg agcttgatcg gccagtacgg
      961 ccacaccagc ggcttgttct cggtgattgg cggctgggtg agcagctcga actgcgtgac
     1021 cgacagcgcg ccctggcgcg tcgacgtgcc gacgcaatcg gacccggtat cgccgccgcc
     1081 cacgacgatc acgcgcttgc ccgcggcact gatctgcgcg gtcaccgcgt cgcctgcaac
     1141 cagacgattc tgctgtggca ggaactccat tgcgaaatgc acgccgcgca gcgcgcgccc
     1201 cggcaccggc aggtcgcgcg gcttctcggc gccgcccgag agcgcaatcg cgtcgaactc
     1261 gccgagcagc gcctgtgccg agacgttgcc gccgacgtgt gaattcggat ggaactcgac
     1321 gccttcgatc gacatctgcg ccatgcgccg gtcgatcagg tgcttctcca tcttgaagtc
     1381 gggaatgccg tagcgcagca ggccgccgat gcggtcctct ttttcgaaca ggacgacgtc
     1441 atgtccggca cgcgccagtt gctgcgcaca ggcaagtccg gcagggcccg aaccgacgac
     1501 ggccacgcgc cggccggtct tctgcaccgg tggcagcggc acgacccagc cctcgtcgaa
     1561 gcccttgtcg atgatgaagt gctcgatcga cttgatgccg acgggatcgt cgttgatgtt
     1621 gagcgtgcac gccgcctcgc acggcgccgg acagatgcgt ccggtgaatt cggggaagtt
     1681 gttcgtcgag tgcagcacgt cgagcgccgc cttccactgg tgccggtaga cgaggtcgtt
     1741 ccagtcggga atgatgttgt tgaccggaca gccggtctgg cagaacggga tgccgcaatc
     1801 catgcagcgt ccgccctgcg tcgacgcggc cgcgtcgtcg agcgcgtgga tgaactcgcg
     1861 gaagtgacgc acgcgcgagc gtacggggtc ggacacttcg gcgatccgct gccattcgag
     1921 aaagccggtg atcttgccca tcgtcgccag cccctacgcg accgcgcgct gtgcgggaac
     1981 gcccgcaggc ttcgcgtgct gcgccttcac gtgcatttcc gatagcgcgc gcacgtactc
     2041 gtttgggaac accttcacga atttcgcgcg cgtcgcgtcc cagcggtcga gcatcgacag
     2101 cgcgagcgtc gatcccgtga agcgcagatg gcgctcgatc aggcggcgca ggatcgcttc
     2161 gtcggccacg ccttcgtgca gcaggcgtcc cttgcccgcg ctcacgagct ccttttccac
     2221 gcgctgctgc tcgctttcgg aaagcaccgg cccgagtgcc accatcgccg ggttgcagcg
     2281 cgatgcgaaa ctgccgtcgg catcgaacac gtaggcgaca cccccgctca ttccggccgc
     2341 gaaattgcga ccggtgcggc cgagcacgac gacggtcccg cccgtcatgt attcgcagcc
     2401 atgatcgccg gtgccttcga cgaccgcgct tgcgcccgaa ttgcgcacgc agaagcgctc
     2461 gccggcgatg ccgcggaaat acgcctcgcc ggcaatggcg ccgtacatca ccgtgttgcc
     2521 gatgacgatg ttctcctcgg ccttcgccgg gcacgcggga tcgggataga cgacgatgcg
     2581 cccgccggac agtcccttgc cgacgtaatc gttggtgccg ccctgcaatt cgaacgtgat
     2641 tccgcgggcg aggaatgcac cgaagctctg tccggcgatg ccctcgaacg cgacgtgcag
     2701 cgtgtcttcg gcgagcccgt cgtggccgtg cctgcgcgca accaccccgg agagcatcgc
     2761 gccgacggaa cggttcgcat tgcggattcg cgaggcgagc cgcaccggtt gtcccttgtc
     2821 gattgccggt gccgccgctt cgatcagttg atgatcgagc gcgcgcgcaa ggccatggtc
     2881 ctgcgtctcg cagtgatgcc gcgcgacctc gggcggcatc ggcggctggt ggaacacgcg
     2941 gctgaaatcg agtcccttcg ccttccagtg ctcgatgccc tttttcgtat cgagcaggtc
     3001 actgcgcccg atcatctcgg acagcgtgcg gaagccgagc ccggccatga tggcgcgcaa
     3061 ctcctccgcc acgaagaaga agtaattgac gacgtgctcc ggctggccgg tgaagcgccg
     3121 cctgagcacg gggtcctgcg tcgcgacgcc gaccggacac gtgttgaggt ggcacttgcg
     3181 catcatgatg cagccttcga cgaccagcgg cgccgtcgcg aagccgaact cgtccgcacc
     3241 gagcaacgcg ccgatcgcga cgtcgctgcc ggtcttcatc tggccgtcga cctgcacggc
     3301 gatgcgcccg cgcaagcgat tgagcacgag cgtctgctgc gtctccgcaa gtccgagctc
     3361 ccacggcgtg cccgcgtgct tgatcgacga ttccggcgag gcaccggtgc cgccgtcgtg
     3421 gcccgcgatc gtgacgtgat ccgccttggc tttcgcgacg cctgcggcca ccgtgccgac
     3481 accaacctcg gagacgagct tcaccgagat cgacgcccgc ggattcgcgt tcttgaggtc
     3541 gtggatgagc tgcgcgagat cctcgatcga atagatgtcg tgatgcggcg gcggggagat
     3601 gagacccacg ccgggcaccg aaaagcgcaa ctgcgcgatg tattcggaca ccttgtggcc
     3661 aggaagttgg ccgccctcgc ccggcttcgc accctgcgcc atcttgatct gcaactgatc
     3721 ggccgacgcg aggtattcgg cggtgacgcc gaatcgggcc gaggcgacct gcttgatgcg
     3781 cgagcgcagg ctgtcgccgg ccttgagcgc aatgtcgtgc tcgaccggac ccttgccgat
     3841 gatcgtcgcg agcgtgtcgc cgtcctcgac gacgcttttt cccgcgcgca tctcggcgcg
     3901 atagcgcatc gggtcctcgc ccccttcgcc ggtgttcgac ttgccgccga tgcgattcat
     3961 cgcgatggcg agcgtcgtgt gcgcttcggt cgagatcgag ccgaggctca tcgcgcccgt
     4021 cgcgaagcgc ttgacgagca ccgatgccgg ctccacctcg tcgatcggca ccggcgtgcg
     4081 cccggccgtt ttgaactcga acagcccgcg caacgtcttc agcctgcggc cctgctcgtt
     4141 gatgagcgtc gcgtattcct tgtaggtcga gtgcgaattc gcgcgcgccg cgtgctggag
     4201 cttcgcgatc gtgtcgggcg tccacatgtg ctcttcaccg cggatgcggt acatgtaatc
     4261 gccgccggca tcgagtgcgc ctgcgagcag tggatcgttg ccgaacgcga gcgcgtgcag
     4321 ccgctcggct tcggccgcga cctcgaagag accaatcccc tcgatcgtgc tcgcggtgcc
     4381 cgtgaagtac ttgtcgacga acgccttctg caggcccacc gcctcgaaga tctgcgcgcc
     4441 gcagtacgac tggtatgtcg agatgcccat cttcgacatc accttgcaga ggcccttgca
     4501 gatcgccttg atgtagcgct tctgtgcctc ctttccggcg tcggccgaga gctcggcgag
     4561 ggtctcgagc gcgagatacg ggtggatggc ttccgcgccg tagcctgcga gcagcgcgaa
     4621 atgatggacc tcgcgcgcgg accccgtttc gacgacgagg cccgtcgacg tgcgcagtcc
     4681 cttcttcacc agatgctcgt gcaccgccgc ggtcgcgagg agcgccggga tcggcagcat
     4741 gtcggcgccg atcatgcggt cggacaggat caggatgttg tagccctgat ggatcgcatc
     4801 ctcggcgtgc gcggcaaggc ttgcgagcgc cgcttccatg ccgttgccgc cccacgcgga
     4861 cgggtagcag atgtcgagct cgatcgagcg aaaggcgccg cccgagaaaa gctccacgtg
     4921 gcgaagcttc tccatgttct ccggtgtcag caccggctgc tgcgcctcga gccgcatcgg
     4981 cggctcgcca tcgggcgtcg tgccgtgccc gtgcacgccg ttcgcgatct cggcgtcgtc
     5041 gtcgcgcgga tcgagcgcca ggagattggg acgcggtccg atgaacgaga cgagcgacat
     5101 gacgagctcc tcgcggatcg gatcgatcgg cgggttcgtc acctgtgcga agagctgctt
     5161 gaaatagttg tagaagacct tcggccgatc ggagagcacc ggcagcgcgg cgtcgttgcc
     5221 catcgagccg accggttcct cggcattcgc cgccatcggc gcgaggatga tcttctggtc
     5281 ttcctgcgtg tagccgaacg cctgctgccg atcgagcagc ggcaccgacg tcggcgtggt
     5341 gggcgccggc tcgggcatct cctcgagccg cagccggtaa cgcgcgatcc agtcgcgata
     5401 cggcttggcg gtggcgagcg ttcgcttcaa ctcgtcgtcg tcgacgatgc gccccgcgtc
     5461 gaggtcgacg agcagcatct tccccggctg caggcgccat ttgcgcacga ccttcgattc
     5521 tggaatgtcg aggacgcccg actcggagga catcacgatg aagtcgtcgt cggtcacgat
     5581 gtagcgcgcg ggcctgaggc cattgcgatc gagcgtcgcg ccgatctggc ggccgtccgt
     5641 gaacgccatc gctgcggggc cgtcccatgg ctccatcagc gccgcgtgat attcatagaa
     5701 ggcgcgccga tcttcgtcca tcagcggatt gccggcccat gcctcgggaa tcatcagcat
     5761 catcgcgtgc gccggcgagt agccgcccat caccagaagc tcgaggcaat tgtcgtacga
     5821 cgccgagtct gactggccgt cgtagatcag cggccacagc ttctgcaagt cgtcgcccag
     5881 aaccttggac gcaattgcac cttcacgtgc gcgaatccag ttgaagttg
//
LOCUS       DASSVX010000178         7344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_773695, whole genome shotgun sequence.
ACCESSION   DASSVX010000178 DASSVX010000000
VERSION     DASSVX010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773695"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VFO53_07615"
     CDS             <1..538
                     /locus_tag="VFO53_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HET8876179.1"
                     /translation="TVARVLDDRRALAVAVLGGGKHDVMLFSSGGKAVRFDENDVRPM
                     GRNAHGVRGMMLDKGQKVIAMLVAEDEEQSVLTATENGYGKRTPIVEYTRHGRGTKGM
                     IAIQQSSRNGQVIGAALVRPDDEIVLISTGGVLIRMGVKSIREMSRSTQGVTLIHLDK
                     GEKLAGLERVVEKDDEEV"
     gene            622..1707
                     /gene="serC"
                     /locus_tag="VFO53_07620"
     CDS             622..1707
                     /gene="serC"
                     /locus_tag="VFO53_07620"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HET8876180.1"
                     /translation="MTLRVWNFSAGPAAMPEPVLQRAQQELLDWHGRGMSIMEMSHRS
                     ADFGAVMTKTESDLRALLAIPANYRVLFLQGGALGQNAAVPLNLLPADGVADYVDTGH
                     WSQKSIAEARKYGRVNVAASGADAQYTLVPAQSGWRLSRDASYVHVTTNETIGGVEYS
                     WVPDTGNVPLVADASSHFLSRPLDVAKFGVIYAGAQKNAGPAGLTLVVVRDDLTGRAR
                     RECPSVFDYAVQAGAASMFNTPSTFAIYLAGLTFEWLAAQGGLAAIERANIAKASALY
                     AQIDASGFYRNPVRHADRSRMNVPFFLHDDRLNEPFEAGAAARGLSQLKGHRAVGGMR
                     ASIYNAMPIEGVHALIDWMREFEREHG"
     gene            1700..2806
                     /gene="pheA"
                     /locus_tag="VFO53_07625"
     CDS             1700..2806
                     /gene="pheA"
                     /locus_tag="VFO53_07625"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET8876181.1"
                     /translation="MADLPSTDDDAREAPLRVHRDAIDALDREILARLSERAMHAQAI
                     GALKADSGSPAWRPEREADVLARLCEQNAGPLSDEAVIGVFRQVMSACLALEQRLRVA
                     YLGPAGTFSHAAVARRFGTFVDAVPCATIDEIFRAGEAGRTDYAIVPVENSTEGTVGR
                     TLDLMVTTDLSICGEIKLRVQQNLLSNAPGLDRVIKVYSHAQSLAQCAQWLARHLPGV
                     ERVPVSSNAEAARLAALETGAAAIAGDIAAPIYGIAVLAPHIEDEPNNTTRFWVLGRQ
                     APGVSGRDETSLVMSAANQPGAMHALLEPFARHGVSMSRIESRPARTGLWEYLFFVDL
                     VGHRDDAPVNAALTELARKAQFLKLLGSYPADQA"
     gene            2803..3945
                     /gene="hisC"
                     /locus_tag="VFO53_07630"
     CDS             2803..3945
                     /gene="hisC"
                     /locus_tag="VFO53_07630"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET8876182.1"
                     /translation="MKHSPEHSAVTKAPEYIRAIAPYVGGKPIDEVARELELDPATII
                     KLASNENPRGASPKVQAAIAAALADLTRYPDGNGFALKTALASRLHLAPEQIVLGNGS
                     NDVLELVTQAFLRPGDDAVYSQYAFIVYPLATQARGARGIEVPAKDHGHDLDAMLAAI
                     TRDTRVVFIANPNNPTGTWLAGDVVERFVAAVPRDVIVVLDEAYNEFLDPAERADSSA
                     WISRYPNLVVSHTFSKAHGLAALRVGYGVMDAAIADLLNRVRQPFNVNSLAQAAALAA
                     LDDTQYVEDSRVLNREGRRQLEAGLQALGLRYLPSRGNFVLFEVGDAMAVYQALLRQG
                     VIVRPVANYGLPAWLRVTVGTPRENERFLASLARSTAASSDTARAH"
     gene            3935..4837
                     /locus_tag="VFO53_07635"
     CDS             3935..4837
                     /locus_tag="VFO53_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET8876183.1"
                     /translation="MRIEKLVVIGVGLIGASCALAVRRACAVGEIVGVGRSRSNLDRA
                     LARGVIDRAAMLGDDWTREVQGADFVLIAIPVAQYRETLAALAPALGRDAIVTDAGST
                     KQDVVLAARELLNERLPQFVPGHPIAGSERSGADAGDASLYVGRQVILTPLPETSSTA
                     LKRVEEFWTTCGARVERLDPARHDRILAAVSHLPHLLAFALVAELASRKDSAEYFSNA
                     GSGFRDFTRIAGSSPEMWRDVALANRDALLTELGAYRSAIEALMLSIEHGDRASLDTM
                     LGRAAEARRHWGETATGVGPSGRA"
     gene            4824..6152
                     /gene="aroA"
                     /locus_tag="VFO53_07640"
     CDS             4824..6152
                     /gene="aroA"
                     /locus_tag="VFO53_07640"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009921655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET8876184.1"
                     /translation="MDALSTSSTGVPDVARLEVGPIRHAEGVVALPGSKSISNRTLLL
                     AAVAEGTTTLTGLLRADDTSRMLDALHTLGVRVDARDAQCTVEGIANAFPQRAATLFL
                     GNAGTAVRPLTAVLAMLGGDYRIAGVPRMHERPIGDLVDALRHQGCDVTYLGNAGYPP
                     LRLGPSGGRAGGRVPIRGDVSSQFLSALLMALPLAQGADASATDVALTTALISRPYVE
                     ITLRLMRRFGVDVASPDASTFVVPAGARYRSPGCMHVEGDASAASYFLAAGVIGGGPV
                     RVEGVGRDSIQGDVAFADVLARQGADIRYGNGWIEARGARPLQGGTIDCIAIPDAAMT
                     LAVVALFARAPIRLVNIGSWRVKETDRIAAMATELAKLGATLAAGNDWLEVSPLATFR
                     HATIDTYDDHRMAMCFALAALGGSGVTINDPGCVRKTFPGYFGSLAAIAH"
     gene            6161..6823
                     /gene="cmk"
                     /locus_tag="VFO53_07645"
     CDS             6161..6823
                     /gene="cmk"
                     /locus_tag="VFO53_07645"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET8876185.1"
                     /translation="MAPVVAIDGPAASGKGTIAYNVARTLGFHYLDSGSLYRLVALRA
                     IEQDVPLDDAVALARLTGGSVISFSAGRAWLDGRDVADALRAENVSAAASRIAVHPDV
                     RGALLARQRAFRMPPGLVAEGRDMGSVVFPDARPKVFLTASAEARAERRHKQLIAKGI
                     SVNIDGLLRDIRERDIRDSTRAAAPLVAAPDAVSLDTTDLSIAEVTAAVLALYRGQAA
                     TH"
     gene            7024..>7344
                     /locus_tag="VFO53_07650"
     CDS             7024..>7344
                     /locus_tag="VFO53_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET8876186.1"
                     /translation="MATATQVATPSAGAENFAALFEESLARQEMRQGELITAEVIRVD
                     YNVVVVNAGLKSESFIPIEEFRDDKGEVEVKPGDFVTVAIESLENGYGETRLSRDKAK
                     RLKAW"
BASE COUNT         1161 a         2460 c         2520 g         1203 t
ORIGIN      
        1 gaccgtggcg cgtgtactcg acgatcggcg tgcgcttgcc gtagccgttc tcggtggcgg
       61 caagcacgac gtcatgctgt tcagctcagg cggcaaggcg gtgcgcttcg acgagaacga
      121 cgtgcgcccg atgggacgca acgcgcacgg cgtccgcggc atgatgctcg acaagggcca
      181 gaaggtcatc gcgatgctgg tcgccgagga cgaggagcag tcggtgctga cggccaccga
      241 gaatggctac ggcaaacgta cgccgatcgt cgaatacacg cgccacggtc gcggcacgaa
      301 ggggatgatc gcgatccagc aatcgagccg caacggtcag gtgatcggcg cggcactcgt
      361 gcgccccgac gacgagatcg tgctcatttc caccggcggg gtgctgatcc gcatgggcgt
      421 gaagtcgatc cgcgaaatga gccgctcgac gcagggtgtg acgctgatcc acctcgacaa
      481 gggcgagaag ctcgccgggc tcgagcgcgt cgtcgaaaaa gacgacgagg aagtctgagt
      541 gcggggattg ccctcacccc aacccttttc cgcgagcggg agagggtgcg gcgaagccgc
      601 gggtgacggg gaacatcgcc catgaccctg cgcgtctgga acttcagcgc gggcccggcg
      661 gcgatgcccg agccggtgct gcagcgtgcg cagcaggagc tgctcgactg gcacggccgc
      721 ggcatgtcga tcatggagat gagccatcgc agcgccgatt tcggcgcggt gatgacgaag
      781 accgaatccg atcttcgcgc gctgctcgcg attccggcga attatcgcgt gctgttcctg
      841 cagggcggcg cgctcgggca gaacgcggcg gtgccgctca atctcctgcc ggccgacggc
      901 gtggccgact acgtcgatac cggccactgg tcgcagaagt cgatcgccga agcgcgcaaa
      961 tacggccgcg tcaacgtcgc cgcgtcgggc gccgatgcac agtacacgtt ggttcccgcg
     1021 cagtccggct ggcgcctgtc gcgcgacgcg agctacgtgc acgtcacgac gaacgagacg
     1081 atcggcggcg tcgagtattc gtgggtgccc gacaccggca acgtgccgct cgtggccgac
     1141 gcgtcgtcgc acttcctttc gcgaccgctc gacgtcgcga agttcggcgt gatctatgcc
     1201 ggcgcgcaga agaacgccgg tcctgcgggc ctcacgctcg tcgtcgtgcg cgacgatctc
     1261 accggccgcg cgcggcgcga gtgcccgagc gtgttcgact acgcggtcca ggcgggtgcg
     1321 gcgtcgatgt tcaacacccc gtcgaccttc gcaatctacc tggccgggct caccttcgag
     1381 tggctcgcgg cgcagggcgg gctcgcggca atcgagcggg cgaacatcgc gaaggcgagc
     1441 gcgctgtatg cgcagatcga cgccagcggc ttctaccgca atccggtccg gcacgccgat
     1501 cgttcgcgga tgaacgtgcc ctttttcctg catgacgatc gcctgaacga gccgttcgag
     1561 gccggtgcgg ccgcccgcgg cctgtctcag ctcaagggcc accgcgcggt cggcggcatg
     1621 cgcgcgtcga tctacaacgc gatgccgatc gaaggcgtgc acgcgctgat cgactggatg
     1681 cgcgagttcg agcgggagca tggctgacct tccgtccacc gacgacgatg cgcgcgaggc
     1741 gccgctgcgc gtgcaccgcg acgcaatcga cgcgctcgac cgggaaatcc tcgcgcggtt
     1801 gtccgagcgt gcaatgcatg cgcaggcgat cggcgcgctg aaggccgaca gcgggtcgcc
     1861 cgcgtggcgg cccgagcgcg aggcggacgt gcttgcccgg ctgtgcgagc agaacgcggg
     1921 accgctttcc gacgaagcgg tgatcggcgt cttccgccag gtgatgtcgg cgtgcctcgc
     1981 actcgagcag cggcttcgcg tcgcctatct cggtcccgcg ggcacgttca gccatgccgc
     2041 cgtggcgagg cggttcggca cgttcgtcga cgccgtgccg tgcgcaacga tcgacgagat
     2101 ctttcgcgcg ggcgaggcgg gccgcaccga ttacgcgatc gtccccgtcg agaactcgac
     2161 cgagggcacc gtcggacgca cgctcgacct catggtcacg accgaccttt cgatctgcgg
     2221 cgagatcaag ctgcgcgtgc agcagaacct gttgtcgaat gcgcccgggc tcgatcgcgt
     2281 catcaaggtc tattcgcatg cgcaatcgct cgcgcagtgc gcacagtggc tcgcgcgtca
     2341 tctgcccggt gtcgagcgcg tgcctgtgtc gagcaatgcc gaagcggcgc ggctcgcagc
     2401 actcgaaacg ggggccgccg ccattgccgg cgatatcgcc gcgccgatct acggcattgc
     2461 ggtgctcgcg ccgcacatcg aggacgagcc gaacaacacg acgcgcttct gggttcttgg
     2521 ccggcaggcg ccgggcgtgt cgggtcgcga tgagacgtcg ctcgtgatgt cggccgcgaa
     2581 ccagccgggc gcaatgcacg cccttctcga accgttcgcg cgtcatggcg tgtcgatgtc
     2641 gcgcatcgaa tcgcggcccg cacgcaccgg cttgtgggaa tatctgttct tcgtcgatct
     2701 cgtcggccat cgtgacgacg caccggtgaa cgccgcgctg accgagttgg cgcgcaaggc
     2761 gcagtttctc aagcttctcg gctcgtatcc cgcagatcaa gcatgaagca ttccccagag
     2821 cactcggcag tgacgaaggc gccggaatac atccgtgcga ttgcaccgta cgtgggcggc
     2881 aagccgatcg acgaggtggc gcgcgagctc gagctcgatc cggccacgat catcaagctt
     2941 gcgtcgaacg agaatccgcg cggcgcgagc ccgaaggtgc aggccgcgat cgccgccgcg
     3001 ctcgccgatc tcacgcgtta ccccgacggg aacggcttcg cgctgaagac ggcgctcgcg
     3061 tcgcgcctgc atctcgcacc ggaacagatc gtgctcggca acggcagcaa cgacgtgctc
     3121 gagctcgtca cgcaggcgtt cctgcgcccg ggcgatgatg ccgtctattc gcaatatgca
     3181 ttcatcgttt atccgcttgc gacgcaggcg cgcggcgcgc ggggcatcga agtgccggcg
     3241 aaggaccatg gtcacgatct ggatgcgatg ctcgcggcaa tcacgcggga cacccgggtc
     3301 gtcttcatcg ccaatccgaa caatccgacg ggaacctggc tcgcgggtga cgttgtcgaa
     3361 cgcttcgttg ccgcggttcc gcgcgacgtg atcgtcgttc tcgatgaggc ctacaacgaa
     3421 ttcctcgatc ccgccgagcg cgccgattcc tcggcatgga tttcgcggta cccgaacctc
     3481 gtcgtgtcgc acaccttctc gaaggcgcac ggactcgcgg cacttcgcgt gggctacggc
     3541 gtgatggacg cggcgatcgc cgatctgctc aatcgcgtgc ggcagccgtt caacgtgaat
     3601 tcgctcgcgc aggcggcggc ccttgcggcg ctcgacgata cgcaatatgt cgaggacagc
     3661 cgcgttctca atcgcgaggg aagacggcag ctggaagcgg gactgcaggc gcttggtctg
     3721 cgttatcttc cgtcgcgcgg caacttcgtg ctgttcgaag tgggcgacgc gatggcggtc
     3781 taccaggcgc tgctgcgcca gggcgtgatc gttcgtccgg tggccaatta cggcctgccg
     3841 gcgtggctgc gcgtcaccgt gggtacgccg cgggaaaacg agcgcttcct cgcgtcgctc
     3901 gcccgctcca ccgcggcgtc gagcgacacg gcccgtgcgc attgagaagc tcgtcgtcat
     3961 cggcgtcgga ttgatcggcg catcgtgcgc gcttgcagtg cgccgcgcat gcgccgtcgg
     4021 cgagatcgtc ggcgtcggcc gttcgcggtc gaatctcgac cgtgcgctcg caagaggcgt
     4081 gatcgaccgt gcggcgatgc tcggcgacga ctggacgcgc gaagtgcagg gggccgactt
     4141 cgtgctgatc gcgatcccgg tcgcgcagta ccgcgaaacg ctcgcagccc tggcacccgc
     4201 gctcgggcgc gatgcgatcg tcaccgacgc gggcagcacg aagcaggatg tcgtgctcgc
     4261 cgcgcgcgag ctcttgaacg agcgcctgcc gcagttcgtg ccggggcatc ccatcgccgg
     4321 cagcgagcgc tcgggcgccg atgcgggcga cgcgtcgctc tacgtcggac gacaggtgat
     4381 cctgacgccg ttgcccgaga catcgagcac tgcgttgaag cgcgtcgagg agttctggac
     4441 cacgtgcggc gcgcgcgtcg agcggctcga ccccgcccgg cacgaccgca tcctcgccgc
     4501 cgtctcgcat ctgccgcatc ttctcgcctt cgcgctcgtc gccgagctcg cgtcgcgcaa
     4561 ggacagcgcc gaatatttca gcaacgcagg aagcggcttt cgcgatttca cgcgcatcgc
     4621 cggcagctcg cccgaaatgt ggcgcgacgt ggcactggcg aaccgcgatg cgctgttgac
     4681 cgagctcggc gcctatcgca gcgccatcga agcgctgatg ctgtcgatcg agcatggcga
     4741 tcgtgcgtcg ctcgacacga tgctcggtcg cgctgccgag gcgcgccggc actggggtga
     4801 aacggcaacc ggcgtcggcc cgagtggacg cgcttagcac gagctccacc ggcgtgcccg
     4861 atgtggcgcg actggaggtc ggtcccattc gccacgcgga aggcgtggtg gcgctgcccg
     4921 gctcgaagag catatcgaat cgcacgctgc tgctcgccgc cgttgccgaa ggcacgacga
     4981 cgctaaccgg gctgctgcgg gcagacgata cgtcgcggat gctcgacgcg ctccacacgc
     5041 tcggcgttcg cgtcgatgcc cgcgatgcgc aatgcacggt cgaaggtatc gccaatgcgt
     5101 ttccgcaacg cgccgcgacg ctgttcctcg gcaacgcggg aaccgccgtg cgtccgctga
     5161 ccgcggtgct tgcgatgctg ggcggcgact accgcattgc gggcgtgccg cgcatgcacg
     5221 agcggccgat cggcgatctc gtcgatgcgc tgcgccatca gggctgcgac gtgacctatc
     5281 tcggcaatgc cggctatccg ccgctccggt tggggccgtc gggcggccgc gcgggcggcc
     5341 gcgttccgat tcgcggcgac gtctcgagcc agttcctgtc ggcgctgctg atggcgctgc
     5401 cgctcgcgca aggcgccgat gcgtcggcca ccgacgttgc gctcaccacg gcgctgatct
     5461 cgcgtccgta tgtcgagatc acgctgcgtc tgatgcgccg cttcggcgtc gacgttgcgt
     5521 cgcccgatgc gtcgacgttc gtcgtcccgg ccggtgcgcg ttaccgcagc ccgggctgca
     5581 tgcacgtcga aggcgacgcc tccgcggcgt cctatttcct tgccgcgggc gtgatcggcg
     5641 gcggaccggt ccgcgtcgaa ggcgtcggtc gcgactcgat ccagggtgac gttgcatttg
     5701 cggacgtact ggcgcgccag ggcgcggata tccgctacgg aaacggctgg atcgaggcgc
     5761 gcggcgcgcg cccgctgcag ggcggcacga tcgattgcat tgcgatcccc gatgccgcga
     5821 tgacgcttgc ggtggtcgcc ctcttcgcgc gtgcgccgat ccggctcgtc aatatcggca
     5881 gctggcgggt caaggaaacg gatcggatcg cggcgatggc caccgagctc gccaagctcg
     5941 gtgcaacgct tgcggccggg aacgactggc tcgaagtgtc gccgctcgcg acgtttcgcc
     6001 acgcgacgat cgacacgtac gacgatcacc ggatggcgat gtgcttcgcg ctcgcagcgc
     6061 tcggcggttc cggcgtgacg atcaacgatc cgggctgcgt gcgcaagacc tttcccggct
     6121 acttcgggtc cctcgcggcc atcgcgcatt gacgggcgca atggccccgg tcgtcgccat
     6181 cgacggaccc gccgcatcgg ggaagggcac gatcgcgtac aacgtcgccc gcacgctggg
     6241 ctttcattat ctcgacagcg gctcgctcta tcgtctcgtc gcattgcgtg cgatcgagca
     6301 ggacgtgccg ctcgacgacg ccgtggcgct cgccaggctg acgggcggca gcgtcatctc
     6361 gttcagcgcg ggccgggcgt ggctcgacgg tcgcgacgtc gccgatgcgc tgcgtgccga
     6421 gaacgtgtcg gccgccgcct cgcgaatcgc cgtgcatccc gacgtgcgcg gcgcgctcct
     6481 cgcacgccag cgggcatttc gaatgccgcc cggactcgtt gcggaaggcc gcgacatggg
     6541 cagcgtcgtc ttccccgacg cgcggcccaa ggtcttcctg accgccagcg ccgaggcgcg
     6601 tgcggagcgg aggcataagc agttgatcgc aaaaggaatc tccgttaaca ttgacggtct
     6661 tttgcgcgat atccgggagc gcgacatccg ggattcgaca cgagcggcgg ccccgctcgt
     6721 ggcggccccc gatgcggtga gcctggatac gaccgatctg tcgatcgcgg aagtgactgc
     6781 ggccgtgctc gccctctatc gcgggcaggc cgcaacgcac tgacgtcgtc gacatggctt
     6841 gcccgtgaac gcgcgggcgc cgcaaggcgt cttcgggttt gacgaacagg attcacgcaa
     6901 agggccgcgg gtgcgcgcta atttagccgg cgcgcacgct acgcggattt caccaacctg
     6961 cccgcctcgg gcgagatgac gggttctcgt tcaaggcggc aaatgcaaaa ggaaccaacc
     7021 cccatggcta ccgccacgca agtcgcaaca cccagtgcag gcgccgagaa tttcgcggcg
     7081 ctgttcgaag aatcgctcgc gcgccaggaa atgcgccagg gcgagctcat caccgcggaa
     7141 gtgatccgcg tcgattacaa cgtcgtcgtc gtcaacgccg gcctcaagtc cgaaagcttc
     7201 attccgatcg aggaatttcg cgacgacaag ggcgaagtcg aggtcaagcc aggcgatttc
     7261 gtcacggtcg ccatcgaaag cctcgagaac ggctacggcg aaacgcgcct gtcgcgcgac
     7321 aaggcgaagc gcctcaaggc ctgg
//
LOCUS       DASSVX010000179         7132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_307064, whole genome shotgun sequence.
ACCESSION   DASSVX010000179 DASSVX010000000
VERSION     DASSVX010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7132
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307064"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..813
                     /locus_tag="VFO53_07655"
     CDS             331..813
                     /locus_tag="VFO53_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876187.1"
                     /translation="MRTSMKGALAAIALTGVLAGCASYDYGYGYAYDRPYYGYGYDYG
                     PYYYGYGPYGYAPGYYYGAPSVGFNLGFRDFDRHDRYDRRDYRRSDRSYRRGHDRGSV
                     RAQQHTYTRPARSNQDVHRQRRTDTTARQRVPAAPPAQRSQAAGNRRAAQAAMRAEQQ
                     "
     gene            814..945
                     /locus_tag="VFO53_07660"
     CDS             814..945
                     /locus_tag="VFO53_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876188.1"
                     /translation="MRLLPAAARAYTIGADAPGAGGDSASFAEALSVDAHMNTAASR"
     gene            1013..1855
                     /locus_tag="VFO53_07665"
     CDS             1013..1855
                     /locus_tag="VFO53_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017288525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET8876189.1"
                     /translation="MSFDVAQATAGLHALIANFSQRLPYMAVALIVFGVFYITGIVVR
                     RTAARFTLRTRRHHSVGIVLGRLGQGMLVLLGLLIALVIAVPGFTPGQLVSVLGLSSV
                     AIGFAFRDILQNFLAGILLLLSEPFRIGDQIKSGDFEGTVENIETRATSIVTYDGRRI
                     VMPNSTLFVNPVTVNTANEKRRLEYDIKLPKDCDIQKMRHLIVRTVAPLPQVLDDPAP
                     DVLVVGFTDSDVTLRVRWWIRPPRHFELQRGLDTVLVHVRDALQAARARSAAPPQSVS
                     KESR"
     gene            1913..3475
                     /locus_tag="VFO53_07670"
     CDS             1913..3475
                     /locus_tag="VFO53_07670"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET8876190.1"
                     /translation="MTLFADVVAASRRVAGTASRNAKITILAQTLRKLAPVEVGVAVA
                     WLSGETRQGRSGVGYALLRDVARGAAANTASLSIGDVDAALDVLAGVSGAGSRAKRTQ
                     LLGDLLDRATDTERDFLFRLLTGELRQGALESVMVDAVAEASALPVALVREAAMVAHG
                     IASVAHAAMTQGAAGLRRYALATMQPIAPMLAQPASDVADALRAFGTAAFEWKLDGAR
                     VQVHKAGDDVRVFTRNRNDVTASAPEIVDAVRASDAAELILDGEAIALKGNGAPEPFQ
                     VTMRRFGRTLDVHAMRESLPLSVFFFDCLRLDADALTTLPASDRFDALSRALPGELLM
                     PRIVTGDIAAAEKFYDDALSRGHEGIMAKSLTAPYEAGRRAPSWLKVKRLHTLDLVVL
                     AAEWGHGRRSGFLSNLHLAARDAESGAFVMLGKTFKGMTDAVLAAQTQALLAREMRRD
                     RWTVYVRPELVVEVAFNDLQASPRYPGGLALRFARIKRYRSDKTAADADTMETVRAIY
                     EGQRARTNDAAG"
     gene            complement(3488..3808)
                     /locus_tag="VFO53_07675"
     CDS             complement(3488..3808)
                     /locus_tag="VFO53_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET8876191.1"
                     /translation="MSDWIDVAPASAFPPGTWRSADVDGTDVAVFNLDGAYYAIENVC
                     THDGGILTGGAVDGDVIVCPRHGARFCIRTGEVLSPPAYEDVTTFPVRVEAGRVQVRD
                     ARWD"
     gene            complement(3853..4224)
                     /locus_tag="VFO53_07680"
     CDS             complement(3853..4224)
                     /locus_tag="VFO53_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HET8876192.1"
                     /translation="MSESTDTERTETTPATDGPQVETARLETNVIAALRTIYDPEIPV
                     NIYDLGLVYGLDVDAAGKVDIRMTLTAPACPVAETFPGTVEQRLYEVPGVSEVRVELV
                     WEPPWNPDRLSEDAKLSLGLL"
     gene            complement(4232..4681)
                     /locus_tag="VFO53_07685"
     CDS             complement(4232..4681)
                     /locus_tag="VFO53_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET8876193.1"
                     /translation="MSGDLRDLYQEVIFDHYKRPRNQGALADPSHHAEGHNPLCGDRV
                     TIYLKVDDGVVQDVTFEGAGCAIATASASLMTEALKGRTLDEVDALFRSFRDMVTDAP
                     AEASPELGKLEVLAGVREFPARVKCATLAWHTLNAALAKRRQPVSTE"
     gene            complement(4678..5931)
                     /locus_tag="VFO53_07690"
     CDS             complement(4678..5931)
                     /locus_tag="VFO53_07690"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET8876194.1"
                     /translation="MEREVFAAPVDRAFDVMRRRADFPILAESVNGHPLVYLDNAATT
                     QKPRSVIDALSRYYAHDNANVHRGVHTLSQRATDDYEAARTNIARFINAGAAEEVVFT
                     RGTTDAINLVAQSFGRPMLRPGDEIVISALEHHSNIVPWQLLCAQTGAVLTVVPVDDA
                     GEFDLDAYASMLGPRTKLVAITHVSNALGTITPIRRIIALARAAGVPVLVDGAQAIAH
                     VAVDVRALDCDFYAFSGHKIFGPTGIGVLYAKAARLEAMPPYQGGGDMIRSVTFEKTE
                     YNDIPYKFEAGTPNIAGAIGLGAALDYVRVVGIDAIEAHERDLLAYATARVSEIPGLR
                     IIGTAKEKAGIVSFTLEGVHAHDVGTILDHGGVAIRAGHHCAMPIMTRFGVPGTARAS
                     FALYNTREEVDALAAGIERVRRMFA"
     gene            complement(5934..>7132)
                     /gene="sufD"
                     /locus_tag="VFO53_07695"
     CDS             complement(5934..>7132)
                     /gene="sufD"
                     /locus_tag="VFO53_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET8876195.1"
                     /translation="RGLPGPRDEDWKYTSVAAIAKRPFAIPVSAGVVDVDTAAFALAG
                     VASHRLVFVDGRYAPSLSQIGRLPAGVTLESLAQTLERAPDALEPVLGDDRYQTVFGA
                     LNAAFMADGVYLHLPRGTVVSEPIQALFVCTAPDAAIHPRNIVVAGEGARATLIEHYV
                     GVDAAAALTNAITQLFVAADASVAHYKVQQEPARALHVGGMHAAQSRASRLESHSIAL
                     GAALARNDITTSFDAEGCEAELNGLFLVRGRQHVDHHTRIDHAKPRGTSREYYKGIVD
                     DAARGVFNGKVIVHPGAQKTNAQLSNHNLLLSKGAEVDTKPELSIDADDVKCTHGATV
                     GQLDDNAMFYLRSRGVDEAMARALLTYAFAHDVIERVRIAPLRAMLERVLVERLPQGG
                     RIRELG"
BASE COUNT         1085 a         2411 c         2471 g         1165 t
ORIGIN      
        1 aggtgcaacg cggcgcgggc aacctcgaaa aggcgtccga caaggccgac gacgacgcac
       61 tcgagtagac ggcagccgcg gtccgtgacg gcgcccgttg ccggcgcccg cggccccctg
      121 cggggtgcgc ccgcggcccc tcgcggggtg ggtccgcggc ccccggcggg tatggcaggc
      181 ccgtgatccg cgcccgttgt cgccatgcgc aacaattgat tggctacgcg cggcggcacg
      241 cgccaccggc gcgctctcca gccacgctcc ctcgcggcgg aacctcccgc gtacgcgcgg
      301 gcctcactcg atatgccgaa ggagaactca atgaggacgt cgatgaaggg agcgctggcc
      361 gcaatcgcgc tgacgggcgt actcgcgggc tgcgcaagct acgattacgg ttacggttac
      421 gcgtacgacc ggccgtacta tggctacggg tacgactatg gcccgtatta ctatggctac
      481 ggcccctacg gctacgctcc gggctattac tacggtgcgc catccgtcgg gttcaatctc
      541 ggtttccgcg acttcgaccg gcacgatcgc tacgatcggc gtgattaccg ccggagtgac
      601 cgctcgtatc ggcgcggcca cgatcgcggc agcgtccgcg cgcagcagca cacgtacacg
      661 cggcccgcgc gttccaacca ggacgtgcat cggcagcggc gtaccgatac cacggcgcgc
      721 cagcgcgtgc cggccgcgcc tcccgcacag cgatcacagg ccgcaggcaa tcggcgcgcc
      781 gcacaggcgg cgatgcgcgc cgagcagcag tgaatgcgcc ttctgccggc ggcggcgcgc
      841 gcgtacacga tcggcgctga tgcgcccggt gcgggcggcg actccgcaag cttcgccgaa
      901 gcgttgtccg tcgacgcgca catgaacacc gcggcaagtc gatgaccgtg gcgctgtcgc
      961 cgcggccggt cgcggccgcg ccggtgtcga tgccgcgcgc ggagcatccc cgatgagctt
     1021 cgacgttgca caggccacgg ccgggctgca tgcgctgatc gccaacttca gccagcggct
     1081 gccgtacatg gcggtcgcgc tgatcgtgtt cggcgtgttc tacatcacgg gcatcgtcgt
     1141 gcgccggacc gcggcgcggt tcacgctgcg tacccgtcgc catcacagcg tcggcatcgt
     1201 tctggggcgg cttggccagg gcatgctggt cctgctcgga ttgctgattg cgctcgtgat
     1261 cgcggtgccc ggattcacgc cggggcaact cgtcagcgtg ctcggcctgt cgagcgtcgc
     1321 catcggcttt gcgttccgcg acatcctgca gaatttcctc gcgggtatcc tgctgctgct
     1381 gtccgagcca tttcgcatcg gcgaccagat caagtcgggc gacttcgagg gaaccgtgga
     1441 gaacatcgag acgcgtgcga cgtcgatcgt cacctacgac gggcgccgta tcgtgatgcc
     1501 gaattcgaca cttttcgtca atccggtgac ggtgaacacc gccaacgaga agcggcgtct
     1561 cgaatacgac atcaagctgc cgaaggattg cgacatccag aagatgcggc acctgatcgt
     1621 gcgcaccgtc gcgccgctgc cgcaggtgct cgacgatccc gcgcccgacg tcctggtcgt
     1681 cggctttacc gacagcgacg tgacgttgcg tgtgcgctgg tggattcgcc cgccgcgcca
     1741 cttcgagctg cagcgcggcc tcgacacggt gctcgttcat gtccgcgatg cgctgcaggc
     1801 ggcgcgtgcg cgttccgcgg cgccaccgca atcggtgtcg aaggagagcc gctgacgacg
     1861 ccgctggcgg gctgcagcga accgcgggcc tcctggcagc gatgatccgc cgatgacgct
     1921 cttcgccgac gtagtggcgg cgtcgcgccg cgtggccggg acagcgagcc gcaacgcgaa
     1981 gatcacgata ctcgcgcaga cgctgcgaaa gctcgcgccg gtcgaggtcg gcgtcgccgt
     2041 tgcctggttg tccggtgaaa cgcggcaggg gcgcagcggc gtcggttatg cgcttcttcg
     2101 cgatgtcgcg cgcggtgccg cagcgaacac cgcgtcgctg tcgatcggcg acgtcgacgc
     2161 ggcgctcgac gtgcttgccg gcgtgtcggg ggccggctcg agggcgaagc ggacgcaact
     2221 gcttggcgac ttgctcgacc gtgccaccga caccgagcgc gacttcctct ttcggctgtt
     2281 gacgggcgaa ttgcgccagg gcgcgctcga atcggtgatg gtcgacgcgg ttgccgaagc
     2341 gtcggcgctg cccgtggcac tggtgcgcga ggcggccatg gtcgcgcacg gcattgcaag
     2401 cgttgcgcac gccgcgatga cgcaaggcgc tgcgggcctt cgccgctatg cgcttgcgac
     2461 gatgcagccg atcgcgccga tgctcgcgca gccggcgagc gacgtggccg acgcgctgcg
     2521 cgcattcggc accgcggcat tcgagtggaa gctcgacggc gcgcgcgtgc aagtgcacaa
     2581 ggcgggcgac gacgtgcgcg tgttcacccg caaccgcaac gacgtgaccg catcggcgcc
     2641 ggaaatcgtc gatgcggtgc gcgcgtcgga cgcggcggag ctgatcctcg acggggaggc
     2701 gatcgccctc aagggcaacg gagcgcccga gccgttccag gtcacgatgc gccgcttcgg
     2761 acgcacgctc gacgtgcacg cgatgcgcga gtcgctgccg ctgtccgtct tcttcttcga
     2821 ctgcctgcgc ctcgacgccg atgcgctcac gacgctgccc gcctccgatc gattcgacgc
     2881 gctgtcgcgc gcacttccgg gcgagctctt gatgccacgc atcgtcaccg gcgatatcgc
     2941 cgcggccgag aagttctacg acgacgcact ttcgcgcggc cacgaaggca tcatggcgaa
     3001 gtcgctgact gcgccgtacg aggcgggcag gcgcgcgccg tcgtggctca aggtgaagcg
     3061 cctgcacacg ctcgacctcg tcgtgctcgc cgccgagtgg ggacatggac ggcgcagcgg
     3121 cttcctgtcg aacctgcatc tcgcggcacg cgacgccgaa tccggcgcct tcgtgatgct
     3181 gggcaagacg ttcaagggga tgaccgacgc ggtgctcgcc gcgcagacgc aggcgctgct
     3241 cgcccgcgag atgcggcgcg accggtggac cgtgtacgtg cgtcccgagc tcgtcgtcga
     3301 ggtcgcgttc aacgatctgc aggcgagccc gcgctatccg ggcgggcttg cactgcgctt
     3361 cgcgcgcatc aagcgctacc gtagcgacaa gacggcggcg gacgccgata cgatggaaac
     3421 ggtgcgcgca atttacgaag gccagcgcgc gcggaccaac gacgcggcag gctgacgcgc
     3481 cgagccgtca atcccagcgg gcgtcgcgca cctgcacgcg gccggcctcg acgcggaccg
     3541 gaaacgtcgt gacgtcctcg tacgcggggg gcgacagcac ctcgcccgtc ctgatgcaaa
     3601 agcgtgcgcc gtgacgcggg cacacgatca cgtcgccgtc gacggcaccg cccgtcagga
     3661 tgccgccatc gtgcgtgcat acgttctcga tcgcgtaata cgcgccgtcg agattgaata
     3721 cggcgacgtc ggtcccgtcg acatcggccg agcgccaggt gccgggcggg aacgcgcttg
     3781 cgggcgccac atcgatccag tcggacatcg gaccccggta gcgtgacgag cgcgcgaacg
     3841 tcccgtacgc tttcagagca acccgagcga aagcttcgcg tcctcggaaa gccgatcggg
     3901 gttccatggc ggctcccaca cgagctcgac gcgaacttcg ctgacgccgg gcacctcgta
     3961 caggcgctgc tcgacggtgc cgggaaacgt ttcggcgacc gggcaggcgg gtgccgtgag
     4021 cgtcatgcgg atgtcgacct tgcccgcggc gtcgacgtcg agcccgtaga cgagcccgag
     4081 atcgtagatg ttgaccggga tctccgggtc gtagatcgtg cgcagcgccg cgatgacgtt
     4141 cgtttccagt cgtgcggttt ccacttgcgg gccgtccgtg gcgggtgtgg tttccgtgcg
     4201 ctccgtgtct gtcgattcgc tcatcgcatt gtcactccgt ggataccggc tgccgccgct
     4261 tcgccagcgc cgcattcagc gtgtgccatg cgagcgtcgc gcatttgaca cgcgccggaa
     4321 attcgcgcac gccggccagc acctcgagct tgccgagctc gggcgacgct tccgccggcg
     4381 catcggtgac catgtcgcgg aaacttcgga acagcgcgtc cacttcgtcg agcgtgcggc
     4441 ccttcagcgc ctcggtcatc aacgacgcgg acgccgtggc gatcgcacag ccggcgcctt
     4501 cgaacgtcac gtcctgcacc acgccgtcgt cgaccttgag atagatcgtg acgcgatcgc
     4561 cgcacagcgg gttgtgaccc tcggcgtggt ggctgggatc ggccagcgcg ccctggtttc
     4621 gcggccgctt gtagtggtcg aaaatgacct cctggtagag gtcgcggagg tcaccgctca
     4681 tgcgaacatc cttcgcacgc gctcgatgcc ggccgcgagc gcgtcgacct cttcgcgcgt
     4741 gttgtacagc gcgaacgacg cgcgcgcggt gccgggcacg ccgaagcgcg tcatgatggg
     4801 catcgcgcaa tggtgtccgg cccggatcgc cacgccgccg tgatcgagga tcgtgccgac
     4861 gtcgtgcgcg tgcacgcctt ccagcgtgaa cgacacgatg ccggcctttt ccttggcggt
     4921 gccgatgatc cgcagaccgg ggatctccga cacgcgtgcc gttgcgtagg cgagaagatc
     4981 gcgctcgtgc gcctcgatcg cgtcgatgcc cacgacgcgg acgtaatcga gcgcggcgcc
     5041 gagaccgatg gcgccggcga tgttcggcgt tcccgcctcg aacttgtacg ggatgtcgtt
     5101 gtactcggtc ttctcgaagg tgaccgagcg gatcatgtcg ccgccaccct ggtacggcgg
     5161 catcgcctcg agacgcgcgg ccttggcgta gagcacgccg atgccggtcg ggccgaagat
     5221 cttgtggccg gagaaggcgt agaaatcgca gtcgagcgcc cgcacgtcga cggcgacgtg
     5281 cgcgatcgcc tgcgcaccgt cgaccagcac ggggacgccg gcggcgcggg cgagcgcgat
     5341 gatcctgcgg atcggcgtga tcgtgccgag cgcattggat acgtgcgtga tcgcgacgag
     5401 cttcgtgcgc gggccgagca tcgacgcgta ggcatcgaga tcgaattcgc cggcatcgtc
     5461 gaccggtacg accgtaagga cggcgccggt ctgggcgcac aggagttgcc acgggacgat
     5521 gttcgagtgg tgctccagcg cgctgatgac gatttcgtcg cccgggcgaa gcatcggcct
     5581 tccgaagctc tgtgcgacga ggttgatcgc atcggtggtg ccgcgcgtga acacgacttc
     5641 ctcggccgcg ccggcgttga tgaagcgcgc aatgttcgtt cgtgcggcct cgtagtcgtc
     5701 ggttgcgcgc tgggaaagcg tatgcacgcc gcgatggacg ttcgcgttgt cgtgcgcgta
     5761 gtaccgggac agcgcatcga ttaccgaccg cggcttctgg gtcgtcgccg cattgtcgag
     5821 atagacgagc ggatgaccgt tcacgctttc ggccaggatc ggaaaatcgg cgcgccggcg
     5881 catcacgtcg aacgcgcggt cgacgggtgc tgcgaagact tcgcgctcca ttttcatccg
     5941 agctcccgga tgcgcccgcc ctgagggagc cgttcgacca gcacgcgctc cagcatggcg
     6001 cgcaacggtg cgatgcgcac gcgctcgatc acgtcgtgcg cgaacgcgta ggtgagcagc
     6061 gcccgcgcca tcgcctcgtc gacgccgcgc gagcgcaggt agaacatcgc gttgtcgtcg
     6121 agctgcccga cggtggcgcc gtgcgtgcat ttgacatcgt cggcgtcgat cgaaagctcc
     6181 ggcttcgtgt cgacttcggc gcctttcgaa agcagcaggt tgtgattcga aagctgcgca
     6241 ttcgtcttct gcgcccccgg atgcacgatc accttgccgt tgaatacgcc gcgcgccgca
     6301 tcgtcgacga tgcccttgta gtactcgcgg ctcgtgccgc gcggcttggc atggtcgatg
     6361 cgcgtatggt gatcgacgtg ctggcgtccg cgcacgagaa agagcccatt gagctcggcc
     6421 tcgcagccct ccgcatcgaa cgacgtcgtg atgtcgtttc gcgccagcgc ggcgccgagt
     6481 gcgatcgagt gcgactcgag gcggctcgcc cgcgactgcg ccgcatgcat gccgccgaca
     6541 tgcagcgcgc gcgccggctc ctgttgcacc ttgtagtgcg cgaccgatgc gtcggccgcg
     6601 acgaagagct gcgtgatcgc gttggtcagc gccgcggcgg cgtcgacgcc gacgtaatgc
     6661 tcgatcagcg tcgcgcgtgc gccttcgccc gcgacgacga tgttgcgcgg atggatcgcc
     6721 gcgtcgggcg ccgtgcagac gaaaagcgcc tggatcggct cggagacgac cgtgccgcgc
     6781 gggaggtgaa gatagacgcc gtcggccatg aacgcagcat tgagcgcccc gaacacggtc
     6841 tggtagcggt cgtcgccaag caccggctcg agcgcatcgg gcgcgcgctc gagcgtctgc
     6901 gcgagacttt ccagcgtcac gcccgccggc aggcggccga tctgcgacag gctcggcgca
     6961 tatcggccat cgacgaacac cagccgatgc gaggccacgc cagccagcgc gaaagcggcc
     7021 gtgtccacgt cgacgacgcc cgcgctcacg gggatggcga acgggcgctt cgcgattgcc
     7081 gcgacgctcg tgtatttcca gtcctcgtcg cgcgggcctg gcaggccgcg ct
//
LOCUS       DASSVX010000180         5064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_174136, whole genome shotgun sequence.
ACCESSION   DASSVX010000180 DASSVX010000000
VERSION     DASSVX010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174136"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1419
                     /locus_tag="VFO53_07700"
     CDS             169..1419
                     /locus_tag="VFO53_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HET8876196.1"
                     /translation="MVSPYELAVGLRYTRARKGSGRNTFISFIALTSMAGIALGVAAL
                     IVVLSVMNGFQQELRNRILSVASHVEIRGYPDLRRPDTIAAEARKNPRVVATAPYVNG
                     QAMLSAGDVNRGALLRGIVPADEEKVADIGNHMRRGTLDSLTPGAFNIVLGAELARAL
                     GVQVGESVVVITPQGTITPAGALPRLKSFNVSGIFEVGMFEFDSGLALINLDDAEKLY
                     RLDDPTGVRLKLDDMFAAPAVAAELARSLPEDVEVRDWTRNHANFFRAVAIEKRVMFI
                     ILTLIVAVAAFNIVSAQVMAVTDKRADIAILRTLGAAPSSVMAIFIIQGALIGIIGTL
                     IGVVGGVLLALNVETVVPAIEHTLHIQFLDKSVYYISDLPSDLQIPDVVTIAIIALVL
                     ALLATLYPSFRAARVNPAEALRYE"
     gene            1580..2266
                     /gene="lolD"
                     /locus_tag="VFO53_07705"
     CDS             1580..2266
                     /gene="lolD"
                     /locus_tag="VFO53_07705"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HET8876197.1"
                     /translation="MAWGGLHPVVACRDLRKTYRTGPLDVPVLLGIDLDVAKGERLAI
                     VGASGSGKSTLLHLLGGLDAPSGGEVRVEDVALATLSEGARGALRNRALGFIYQFHHL
                     LPEFTALENVAMPLRIRRVDERKAREEASEMLARVGLENRVRHLPGELSGGERQRVAL
                     ARALVTRPRCVLADEPTGNLDRRNAGAVFELMLELNRVVGTALIIVTHDAELAARTDR
                     TLHLVDGKLQ"
     gene            complement(2380..3018)
                     /locus_tag="VFO53_07710"
     CDS             complement(2380..3018)
                     /locus_tag="VFO53_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET8876198.1"
                     /translation="MTKPPYWDEAARELASRDPVLARLIAASPGLHLTRRGDPFTTLA
                     RAIVGQQISVKAAQSIWERLVNACGATTPPPTLDPVRVQRKRIATLRACGLSERKAEY
                     IRDLARHFASARLDPRIWPALDDEALIEALVDVKGIGRWTAEMFLMFHEMRADVLPVD
                     DLGLQKAIAVHYFDGERPTLAALREFGEKWRPWRSVATWYLWRSMDPVPVEY"
     gene            3176..4960
                     /locus_tag="VFO53_07715"
     CDS             3176..4960
                     /locus_tag="VFO53_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876199.1"
                     /translation="MRSRRQLASGLVALLIAVSAWAADPGKVLRISFPVAETGFDPVR
                     VSDLYSNIVNEAIFERLLTYDYLARPAKLVPMTAETMPEVSDGGRTYVLHLKRGIYFA
                     PDPVFKGKQRELTAEDYVYSFKRFADPANRAPYGFMIQGRIEGLDEAIEEAKKSGKFD
                     YDATIPGIVALDKYTLRFKLTKVDYLFPYTLAHVPFGAVAREVVDAYGADVQAHPVGT
                     GPYMLKEWRRAARIVLEANPNYRKVTWDFAPGDDAWDKDVVAAMKGKTIPQIARVEIS
                     IIEESQSRWLAFQRKELDYLAVPATFIDQALDAANNLKPEWTQQGVTLYRAIDPSVGY
                     TFFNFRDPLIGGFSKEKIALRRAIIMGYNLDQEIRVIARNQEVQAQMPIPYGVVGFDP
                     NYRNVNQYDPILANKLLDYFGYRKGPDGYRTLPDGKPLVLRQATGTAAIDREYSELWK
                     RSMDAIGVRIVFEPGKFSDTLKAAKACHVMMWQASWTADYPDGDNFMQLLYGPNTGES
                     NNGCYESKAYDALYEKSLTLPTDSEERNHLFLDMTRQMEVDGAWSLQSSPIRNQLIRP
                     WVKGYKKHPILQAEFVYLDIDLKGSGNQ"
BASE COUNT          863 a         1693 c         1707 g          801 t
ORIGIN      
        1 gacagcgagc gcatcgccgc caggtcgtgc gatgccagcg ccagcattcc gggaacgacg
       61 aggacgagcg tatccgccat gcgataatgg gccgcgatcc tgcctgcgac gagcgtagcg
      121 taacgcccgc gcatcgcgtc cctggtttcc cagcgtcttt cctgatcgat cgtgtcgccg
      181 tacgaacttg ccgtcggcct gcgttataca cgtgcccgca agggctcggg gcgcaacacg
      241 ttcatctcgt tcatcgcgct cacgtcgatg gccggcatcg cgctcggcgt cgcggcgctg
      301 atcgtcgtgc tgtcggtgat gaacggcttc cagcaggagc tgcgcaaccg catcctgtcc
      361 gtcgcgtcgc atgtcgagat tcgcggctat cccgatcttc ggcgtcccga caccatcgcc
      421 gccgaggcgc gcaagaatcc gcgcgtcgtg gcaaccgcgc cgtacgtcaa cggccaggcg
      481 atgctgtcgg ccggcgacgt caaccgcggc gcgctgctgc gcggcatcgt ccccgcggac
      541 gaggagaagg tcgcggacat cggaaaccac atgcgccgcg gcacgctcga ttcgctgacg
      601 ccgggcgcct tcaacatcgt gctcggcgcc gagctcgccc gcgcgctcgg cgtgcaggtc
      661 ggcgaaagcg tcgtcgtgat cacgccgcag ggcaccatca cgccggcggg cgcgcttccg
      721 cgcctcaaga gcttcaacgt gagcgggatt ttcgaggtcg gcatgttcga gttcgacagc
      781 ggcctcgcac tcatcaatct cgacgatgcc gagaagctct accgcctcga cgacccgacc
      841 ggcgtgcgcc tcaagctcga cgacatgttc gcagcgcccg ccgttgcggc cgagctcgca
      901 cgaagcttgc cggaggacgt cgaggtgcgc gactggacgc gcaaccatgc gaacttcttt
      961 cgcgcggtgg cgatcgagaa gcgcgtgatg ttcatcatcc tgacgctgat cgtcgcggtg
     1021 gcggcgttca acatcgtctc cgcgcaggtg atggcggtga ccgacaagcg ggcggacatc
     1081 gcgatcctgc gcacgctggg cgccgcaccg tcgtcggtga tggcgatctt catcatccag
     1141 ggcgcgctga tcggcatcat cggcacgctg atcggcgtgg tgggcggcgt gctgctcgcg
     1201 ctcaacgtcg agacggtcgt gcccgccatc gagcacacgc tgcacatcca gtttctcgac
     1261 aagagcgtgt actacatcag cgacctcccc tccgatctgc agatcccgga tgtcgtgacg
     1321 atcgcgatca tcgcgctcgt gctcgcgctg ctggcgacgc tgtacccgag cttccgcgcg
     1381 gcgcgcgtca atccggccga ggcgctgcgc tatgagtgac ggcggggcta cgagcggcga
     1441 agggccgccc cgggacgtcc tcgcgagcgg ggcgaagccc aggcccgaag ggggcgaccg
     1501 cgagcgtgga cgcgaggccg acgacacggc actgcgcgcg acgaaacggt tcaaggaggc
     1561 cgaggcgagt tccgccccct tggcgtgggg gggcctccac cccgttgtcg cctgccgcga
     1621 tctgcgcaag acctaccgca ccgggccgct cgacgttccg gtgctgctcg gcatcgacct
     1681 cgacgtcgcg aagggcgagc ggctcgcgat cgtcggcgcg tcgggatccg ggaagagcac
     1741 gctgctgcat ctgctgggcg ggctcgatgc gccgagcggc ggcgaagtgc gcgtcgagga
     1801 cgtggcgctc gccacactgt ccgaaggtgc gcgcggcgcg ttgcgcaatc gggcgctggg
     1861 cttcatttac cagttccatc atctgctgcc cgagttcacc gcactcgaga acgtcgcgat
     1921 gccgctgcgc atccgccgtg tcgacgagcg gaaggcgcgc gaggaggcga gcgagatgct
     1981 cgcccgcgtg ggactcgaaa atcgcgtacg ccacctgccc ggcgagctgt cgggcggcga
     2041 gcggcagcgc gtagcactcg cccgggcgct cgtgacccgt ccgcggtgcg tgcttgccga
     2101 cgagccgacg ggcaacctcg accggcgtaa cgccggcgcc gtgttcgagc tgatgctcga
     2161 gctcaatcgc gtcgtcggca cggcgctgat catcgtcacg cacgatgccg agctcgccgc
     2221 gcggacggac cggacgctgc acctcgtcga cgggaagctg cagtagcggc gcaacaacgg
     2281 acgggcacgg ccagccgctc gcgccacgcc tggtcgatcg ccccgtctcg acgacgcgca
     2341 gggcgaagac gacgcgcgac acgacgccgg ctgcctggat cagtactcca ccgggacggg
     2401 atccatcgac cgccacagat accacgtcgc gacgctgcgc cacggccgcc acttctcgcc
     2461 gaactcgcgc agtgccgcga gcgtcgggcg ctcgccgtcg aaatagtgca ccgcgatcgc
     2521 cttctgcagc ccgagatcgt ccaccggcag cacgtcggcg cgcatctcgt ggaacatgag
     2581 gaacatctcg gccgtccagc ggccgatgcc cttcacgtcg acgagcgcct cgatcagcgc
     2641 ttcgtcgtcg agcgcgggcc agatccgcgg gtcgagccgt gccgacgcga aatgccgcgc
     2701 gaggtcacgg atgtactcgg ccttgcgctc ggacagccca catgcgcgca gcgttgcgat
     2761 gcgcttccgc tgcacacgga ccgggtcgag cgtcggcggc ggcgtggttg cgccacaggc
     2821 gttgacgaga cgttcccaga tcgactgcgc ggccttgacc gatatctgct ggccgacgat
     2881 cgcgcgggcc agcgtggtga acgggtcgcc acggcgggtg agatggagcc cgggactggc
     2941 ggcgatcagc cgcgcgagca ccgggtcgcg cgaggcgagc tcgcgcgcgg cttcgtccca
     3001 gtaaggtggc ttggtcatcg ggaatcggcg cgcggcacct cgccgcagcg gccttgtcgc
     3061 acggcgcgca acgaaatcgc gggtaccgcg gtcatacgcg cgccgcgcaa agcgctcgcg
     3121 cagacggcgg aaatggtaca ttacgccgcc catttttaga gactccggag catggatgcg
     3181 tagtcgtcgg caattggcaa gcggcctcgt cgcgctgctg attgccgttt cggcatgggc
     3241 ggccgacccg gggaaggtgc tgcgcatctc gttcccggtc gccgagacgg ggttcgaccc
     3301 ggtccgcgtt tccgacctct actcgaacat cgtcaacgag gcgatcttcg agcggctgct
     3361 cacctacgac tatctggcgc ggccggcgaa gctcgttccg atgaccgccg agacgatgcc
     3421 cgaagtgtcg gacggcggcc gcacgtacgt gctgcatctc aagcgcggca tctacttcgc
     3481 gcccgacccg gtgttcaagg gcaagcagcg ggagctcacc gccgaggact acgtgtactc
     3541 gttcaagcgc ttcgccgatc cggcgaaccg tgcgccgtac ggattcatga tccagggcag
     3601 gatcgagggt ctcgacgagg cgatcgagga agcgaagaaa tcgggaaagt tcgattacga
     3661 cgcaaccatt cccggcatcg tcgcgctcga caagtacacg ctgcgcttca agctgacgaa
     3721 ggtcgattac ctctttccgt acacgctcgc gcacgtgccg ttcggcgcgg tcgcacgcga
     3781 ggtcgtcgat gcctacggcg ccgacgtgca ggcgcatccg gtgggcaccg ggccgtacat
     3841 gctgaaggaa tggcggcggg ccgcgaggat cgtgctcgag gccaatccga actaccgcaa
     3901 ggtcacctgg gacttcgcgc ccggtgacga cgcgtgggac aaggacgtgg tcgcggcgat
     3961 gaagggcaag acgattccgc agatcgcccg cgtcgagatc tcgatcatcg aggagagcca
     4021 gtcgcgctgg ctcgcgttcc agcgcaagga gctcgactac ctggcggtgc cggcgacgtt
     4081 catcgaccag gcgctcgacg cggcgaacaa cctgaagccc gagtggacgc agcagggcgt
     4141 gacgctgtac cgggcgatcg atccgagcgt tggctacacc ttcttcaatt tccgcgatcc
     4201 gcttatcggc ggcttctcga aggagaagat cgcgctgcgc cgcgcgatca taatgggata
     4261 caacctcgac caggagatcc gggtcatcgc caggaaccag gaggtccagg cgcagatgcc
     4321 gatcccctat ggggtggtcg gattcgatcc gaactatcgc aatgtcaacc agtacgaccc
     4381 gattctggcc aacaagcttc tcgactactt cggttatcgc aagggaccgg atggctatcg
     4441 gacgctgccc gacggcaagc cgctcgtcct gcgccaggcg accggcaccg ccgccatcga
     4501 ccgcgaatac agcgagctgt ggaagcgctc gatggatgcg atcggcgtga gaatcgtgtt
     4561 cgagcccggc aagttctccg acacgctgaa ggccgccaag gcgtgtcacg tgatgatgtg
     4621 gcaggcgtcg tggacggccg actacccgga cggcgacaac ttcatgcaac ttctctacgg
     4681 gcccaacacg ggcgagagca acaacggctg ctacgaatcg aaggcgtacg acgcgctgta
     4741 cgagaagtcg ctcacgctgc cgaccgattc cgaggagcgc aatcacctgt tcctcgacat
     4801 gacgcggcag atggaggtcg acggcgcctg gagcctgcag tcgtcgccga tccgtaacca
     4861 gctcatccgt ccctgggtga agggctacaa gaagcatccg atcctgcagg ccgagttcgt
     4921 gtacctcgac atcgatctca agggttcggg caatcagtga ggacgcgatg acgaaagcga
     4981 tgtctgcgcg caccgatcga tgcccgtccg agtctggcgc gcggcgcgcc ggcgcgcgca
     5041 ggttcggcgt cgcactcgca tcgg
//
LOCUS       DASSVX010000181         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1190612, whole genome shotgun sequence.
ACCESSION   DASSVX010000181 DASSVX010000000
VERSION     DASSVX010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190612"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VFO53_07720"
     CDS             complement(<1..481)
                     /locus_tag="VFO53_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876200.1"
                     /translation="MPPADAARTGTSARNKRILLLLVAIAIAPVVLAYVAYYAWPRDA
                     RVNYGELLSGTLPTITGARLDAMPFRSDALRGRWAVLYAAGGDCGGDCPAALYASRQA
                     RTIQNAERDRVVRVWLVTSSAVPAARLLAEHPDLAVVRIDAPPALPRGTRRIYLVDPL
                     "
     gene            complement(468..1208)
                     /locus_tag="VFO53_07725"
     CDS             complement(468..1208)
                     /locus_tag="VFO53_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HET8876201.1"
                     /translation="MREPPRVRRPKWLPTVAAFVVAALCIVAGNWQHRRMLEKEALQA
                     SITRAAAKAPVALPAGVDDWNAWRFHRVTATGEYDAAHQILIDNKVHAGVVGFDVVTP
                     LRMDDGRTVLVDRGFVPVGRSRATLPRPAPPTGVVTVDGRVDVPPARYFELGDGSAPS
                     GPLWEHLDPARYAKATGMVVLPIVVDATQAGGGEGLVQDFPLPDTGIDRHLSYMVQWY
                     TFAAMAIGLWAWFALVRPRLARRTHAAG"
     gene            1246..1443
                     /locus_tag="VFO53_07730"
     CDS             1246..1443
                     /locus_tag="VFO53_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HET8876202.1"
                     /translation="MRAIVIVALIAVVTALFTALVFLYRDRGRGNRVVIALAVRVVLS
                     MALIAFLVISWYMGWIGPGGL"
     gene            complement(1454..2323)
                     /locus_tag="VFO53_07735"
     CDS             complement(1454..2323)
                     /locus_tag="VFO53_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET8876203.1"
                     /translation="MSAAAAPGAKPYYFIPQPSHWPIMVACALLLMGSGAAFWFNGYA
                     AGPWMVLAGFCVLIFTFFGWFGKVIGESEHRLYNKRVDTSFRWSMSWFIFSEVMFFAA
                     FFGALFYVRNISVPDLGSELTGRVLWPNYAPQWPTVGPYLKQAFTPMEAIGIPLLNTI
                     ILVTSGGTLTLAHHALKAGHRGALKAWLAVTIALGFSFLGLQAYEYTHAYSSLNLKLS
                     TGVYGSTFFMLTGFHGFHVTIGAIMLSVMLVRAFRGHFDAEHHFAFEAAAWYWHFVDV
                     VWLLLFVLVYWLA"
     gene            complement(2393..>2858)
                     /locus_tag="VFO53_07740"
     CDS             complement(2393..>2858)
                     /locus_tag="VFO53_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET8876204.1"
                     /translation="GFGYLLVPFYEKICEVTGLRNIDAADVVTNTQVDATRSVHIEFD
                     TNLRNLPWQFRALQLATDVHPGQVTQAMFEVVNLTDHPITGQAIPSYGPQQAALYFKK
                     IDCFCFAKQTLAPHEKREMPVVFVVDPKLPKDVPTITLSYTFFEVEGNDARS"
BASE COUNT          506 a          955 c          949 g          448 t
ORIGIN      
        1 cgagcggatc gaccaggtag atccttcgcg tcccgcgcgg aagcgccggc ggggcgtcga
       61 tgcggacgac cgcgagatcg ggatgctcgg cgagcagcct tgcagccggt acggcactgc
      121 tcgtcacgag ccagacgcga acgacgcgat cgcgctcggc gttctggatc gtgcgtgcct
      181 gcctcgacgc gtacagcgcg gcgggacagt cgccgccgca gtcgccgccg gccgcgtaga
      241 gcaccgccca gcgcccgcgc aacgcgtcgc tgcgaaacgg catcgcgtcg aggcgcgcgc
      301 cggtgatcgt cggcagcgtt cccgacaaca gctcgccgta gttcacacgc gcatcgcgcg
      361 gccacgcgta gtaggcgacg tacgcgagga cgacgggggc gatggcgatt gccaccagca
      421 gcaggaggat gcgcttgttg cgcgcgctcg tcccggtccg cgccgcgtca gccggcggca
      481 tgcgttcgcc tcgcaaggcg tggacgaacg agcgcgaacc acgcccagag gccgatggcc
      541 atcgccgcga acgtgtacca ctgcaccatg tagctcaaat gccggtcgat gcccgtgtcg
      601 ggcaacggaa aatcctgaac gagaccctcg ccgccgcccg cttgcgtcgc gtcgacgacg
      661 atcggcagca cgaccatgcc cgtcgccttc gcatagcgcg ccggatcgag gtgctcccac
      721 agcggcccgc tcggcgcgct gccatcgccg agctcgaagt aacgcgccgg cgggacgtcg
      781 acgcggccgt cgacggtgac gacacccgtc ggcggcgccg gccgcggcag cgttgcgcgc
      841 gagcgaccga cgggaacgaa gccgcgatcg acgagcaccg tgcgtccgtc gtccattcgc
      901 agcggcgtca cgacgtcgaa tccgaccact ccggcatgca ccttgttgtc gatcaggatc
      961 tggtgcgcgg catcgtattc gccggtcgcc gtgacgcggt ggaaacgcca cgcgttccag
     1021 tcgtcgacgc cggcaggcaa tgcaacgggc gctttagcgg cggcacgcgt gatcgatgcc
     1081 tgcagcgctt ccttctcgag catccgccgg tgctgccaat tgccggcgac gatgcacagc
     1141 gcggcgacga cgaacgcggc aacggtcggc agccacttcg gccggcgcac gcgcggcggc
     1201 tcgcgcattt tcaagtgcta cactggccca agactcgcga tcgtcatgag agccatcgtc
     1261 atcgtcgcgc tgatcgcggt cgtcaccgcg cttttcaccg cgctcgtctt tctctatcgc
     1321 gaccgcggac gcggcaaccg cgtcgtcatc gcgctcgcgg tgcgtgtcgt gctgtcgatg
     1381 gcgctgatcg cgttccttgt catctcgtgg tacatgggct ggatcggccc cggcggcctt
     1441 tagctcgcga gaactacgcc aaccagtaga cgagcacgaa cagcagcagc cacacgacgt
     1501 cgacgaagtg ccaataccac gcggcggcct cgaacgcgaa gtggtgctcg gcgtcaaagt
     1561 gcccgcggaa cgcgcgcacc agcatcaccg acagcatgat cgcgccgatc gtcacgtgga
     1621 agccgtggaa gccggtcagc atgaagaacg tcgacccgta gacgccggtc gacagcttca
     1681 ggttgagcga cgagtacgcg tgcgtgtact cgtacgcctg caatcccagg aacgagaagc
     1741 ccagcgcgat cgtgacggcg agccaggcct tcagcgcgcc gcggtggccg gccttcagcg
     1801 catggtgcgc gagcgtcagc gtgccgccgg aggtcacgag gatgatcgtg ttgagcagcg
     1861 ggatgccgat cgcctccatc ggcgtgaatg cctgcttcag gtacggaccg acggtcggcc
     1921 attgcggcgc gtaattcggc cacaggaccc ggccggtcag ctcgctgccg agatccggca
     1981 ccgagatgtt gcgcacgtag aacagcgcgc cgaagaacgc ggcgaagaac atcacctcgc
     2041 tgaagatgaa ccagctcatg ctccagcgga acgacgtgtc gacgcgcttg ttgtacagcc
     2101 gatgctcgga ttcgccgatg acttttccga accatccgaa aaacgtgaag atcaggacgc
     2161 aaaagccggc gagcaccatc cagggcccgg ccgcatagcc gttgaaccag aaggcggcgc
     2221 cgcttcccat caggagcagc gcgcacgcga ccatgatcgg ccagtgcgaa ggctgcggga
     2281 tgaagtaata cggcttagcg cccggtgcgg cggcagcgct catcggtggc tcccaggggt
     2341 cagagacgag tttctcgctt gcgagcaagt cgtctctgac cccacttcgg ttctaggatc
     2401 tcgcatcatt cccctcgacc tcgaagaacg tgtacgaaag cgtgatcgtc gggacgtctt
     2461 tcggcagctt cggatcgacg acgaacacga ccggcatctc gcgcttctcg tgcggcgcga
     2521 gcgtctgctt cgcgaagcag aagcagtcga tcttcttgaa gtagagcgcc gcctgctgcg
     2581 gcccgtagct cggaatcgcc tggccggtga tcgggtggtc ggtcaggttg acgacctcga
     2641 acatcgcctg cgtgacctgc cccggatgca cgtcggtcgc gagctgcagc gcacggaact
     2701 gccacggcag gttccggagg ttcgtgtcga actcgatgtg cacgctgcgc gtcgcgtcga
     2761 cctgcgtgtt cgtgacgacg tcggcggcgt cgatgttgcg caagcccgtc acctcgcaga
     2821 tcttctcgta gaacgggacg agcaggtagc cgaagccg
//
LOCUS       DASSVX010000182         4722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1357186, whole genome shotgun sequence.
ACCESSION   DASSVX010000182 DASSVX010000000
VERSION     DASSVX010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357186"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFO53_07745"
     CDS             <1..475
                     /locus_tag="VFO53_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HET8876205.1"
                     /translation="FMTREETSKYTDLLPDGKAVQFSLAMEAKSVITSPSGEMVLDGP
                     GFHEITGLAWSGRGSIRRVDVSADGGRTWRAASLQAPVLPICHTRFRFAWTWDGSEAI
                     LQSRCVDDTGYVQPTLGQLIAVRGLNGPLGSIYHLNAIQSWRVANDGKVSNVHHY"
     gene            459..1067
                     /locus_tag="VFO53_07750"
     CDS             459..1067
                     /locus_tag="VFO53_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET8876206.1"
                     /translation="MSITTEVVFALALAVLGAQAHAADAEKQAYAFGTRPSEAELGHF
                     VSPLPDGRGLPQGSGSVLEGKSVYQQQCASCHGEKLQGGLGDRLIGGRGTLVNDSPAK
                     APVKTVESYWPWPTTLFDYIKRAMPMAAPGSLSDDQVYALTAYILSQANIVSEDAIMS
                     ADTLPKVVMPNRDGFIPDHRPEHFPPVARTTHQTLAIAPAAK"
     gene            1064..2371
                     /locus_tag="VFO53_07755"
     CDS             1064..2371
                     /locus_tag="VFO53_07755"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HET8876207.1"
                     /translation="MTTRPKTHRFDTLSLHAGSRPDPATGARATPIYQTSSFVFPDTD
                     HAAALFNMERPGHVYSRLSNPTCAVLEERIAALEGGIGAIATASGQAALHLAIVTLMS
                     AGSHIVASRSLYGGSHNLLDYTLPRFGIETTFVDPRDLDAWAGAIRPTTRLLFGETLG
                     NPGLEVLDIPRVAAIAHAQALPLLVDSTFTTPWLMRPFEHGADLVVHSATKFLSGHGT
                     VIGGLLVDSGAFDWNGDGVRGKFPTLTEPYAGFHDMVFAEESSTGAFLLRARREGVRD
                     FGACMAPMTAFHILQGIETLPVRMARHVENTRAIVAFLREHPFVSGVGYPELTTHPDN
                     ALAARLLPRGAGAVFSFNLRGTREQGRRFIEALRLFSHLANVGDAKSLVIHPASTTHF
                     RMSDEDLRRAGITEGTIRLSIGLEDRADLIDDLSRALNAASKA"
     gene            2371..3177
                     /locus_tag="VFO53_07760"
     CDS             2371..3177
                     /locus_tag="VFO53_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8876208.1"
                     /translation="MRVDVAGASVYAYSGTRAFVRGRPSVVFVHGAANDHGVFAYQSR
                     YFAWHGMNCVAVDLPGHGRSAGDALASVEALADWLRDVFDALGVSDVDLVGHSLGSLA
                     ALECASRHGERVRRLALLGPSVPMPVSDDLLAAASRNDHVAYELINGWSFAAGSQLGG
                     NPVPGMWMLGNAMRLMERTRDGVLSTDLIACNRYANGLEAAARVRCPTLVVLGARDIM
                     APTRNAKALIATLADATTVTLPETGHSMMAERPDQVLDALRRFLPVNPAT"
     gene            complement(3161..3700)
                     /locus_tag="VFO53_07765"
     CDS             complement(3161..3700)
                     /locus_tag="VFO53_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET8876209.1"
                     /translation="MTTPVALGWPAAYFDLPGIDGRRHTPDTARGPKGLLVMFLANHC
                     PYVRAIAAKIARDADALAPLGIGSIAIMSNDAATYPEDSFDNMNVFAAEHRFSFPYVV
                     DESQDVARAYDAVCTPEFFGFDADLTLAYRGRLDASGRSSNPAARRELYEAMAQVART
                     GRAPVEQTPAIGCSITWRG"
     gene            3814..4551
                     /locus_tag="VFO53_07770"
     CDS             3814..4551
                     /locus_tag="VFO53_07770"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET8876210.1"
                     /translation="MTPRIFVGDDVAALAPGVDYVLPQAAARHVAQALRMRTGQPLTL
                     FTGSGGEYATTVAHIDRRQVVTHVERHEAVERESPHAVTLVQSLIAAEMMDFVVRKAV
                     ELGVAAIVPLLAARSQAIAADRAERRVAHWRQVAIAACEQCGRNRVPGVADIVPFGEW
                     AEAIEPAAALAILDFDASRSLAALASTAPPRSVAVGPEGGFAADELRDAGARGAIGVH
                     LGRRMLRAETAAIAALATLNSIAGDAQ"
BASE COUNT          727 a         1615 c         1607 g          773 t
ORIGIN      
        1 gttcatgacg cgcgaggaga cgtcgaaata caccgatctc ctgcccgacg gcaaggcggt
       61 gcagttctcg cttgcgatgg aggcgaaatc ggtgatcacg tcgccgtccg gcgagatggt
      121 gctcgacggc cccggcttcc acgagatcac cggcctcgcg tggtcgggcc gcggcagcat
      181 ccgtcgcgtc gacgtctcgg ccgacggagg gcgcacctgg cgcgcggcgt cgctgcaggc
      241 tcccgtgctg ccgatctgtc acactcgctt ccgctttgcg tggacgtggg atggcagcga
      301 ggcgatcctg caaagccgct gcgtcgacga caccggctac gtgcaaccta cgctcggaca
      361 gctcatcgcc gtgcgcggcc tgaatggtcc gctcggctcg atctatcacc tgaatgcgat
      421 ccagagctgg cgcgtcgcca acgacggcaa ggtgtcgaat gtccatcact actgaagtag
      481 ttttcgcgct ggcgctcgcc gtactcggcg cccaggcgca cgcggccgac gccgagaagc
      541 aggcgtacgc attcggcacg cgaccgtcgg aggccgagct cggtcatttc gtgtcgccgc
      601 tgcccgatgg acgcggattg ccgcaaggct ccggatcggt gctcgagggc aagtcggtct
      661 accagcagca atgcgcgtcg tgccatggag aaaagctgca gggcgggctc ggcgatcggc
      721 tgatcggcgg ccgcggaacg ctcgtcaacg acagtcccgc gaaggcaccg gtcaagaccg
      781 tcgaaagcta ctggccgtgg ccgacgacgc tgttcgatta catcaagcgc gcgatgccga
      841 tggcggcacc cggcagcctc agcgacgacc aggtgtacgc gctgacggcc tacattcttt
      901 cgcaggcgaa catcgtgtcg gaagacgcga tcatgagcgc cgacacgctg ccgaaggtcg
      961 tgatgcccaa tcgcgacggc ttcattcccg accatcgccc cgagcatttc ccccccgtgg
     1021 cacgcacgac gcaccagacg ctcgccattg caccggccgc gaagtgacca cgcgaccgaa
     1081 gacgcaccgt ttcgacacgc tgtcgctgca cgcgggatcg cgtcccgatc cggcgaccgg
     1141 cgcacgcgcg acgccgatct accagacgtc gtcgttcgtc ttccccgaca ccgatcatgc
     1201 ggcggcgctc ttcaacatgg agcgcccggg acacgtgtac tcgcgcctgt cgaatccgac
     1261 ctgcgcggtg ctcgaagagc gcatcgcggc gctcgaaggc ggcatcggcg caatcgccac
     1321 cgcgagcggc caggcggcgc tgcatctcgc gatcgttacg ctgatgtcgg cgggatcgca
     1381 catcgtcgcg tcgcgctcgc tctacggcgg ctcgcacaac ctgctcgact acacgctgcc
     1441 gcgcttcggt atcgagacga cgttcgtcga tccgcgcgac ctcgacgcgt gggccggcgc
     1501 catccggcca accacgcggc tcctgttcgg cgaaaccctc ggcaaccccg ggctcgaggt
     1561 gctggacatc ccgcgcgttg cagcaattgc gcacgcgcag gcgctgccgc tgctcgtcga
     1621 ttcgacgttc accacgccgt ggctgatgcg cccgttcgag catggcgccg atctcgtcgt
     1681 gcattcggca accaaattcc tttccggaca cggcacggtg atcggcggac tgctcgtcga
     1741 ttcgggcgcg ttcgactgga atggcgacgg cgtgcgcggg aagtttccga cgctgaccga
     1801 gccgtacgcg ggattccacg acatggtgtt cgccgaggaa tcgtcgaccg gcgcattcct
     1861 gctgcgcgcc cgccgcgagg gcgtgcgcga cttcggcgcg tgcatggcgc cgatgacggc
     1921 gttccacatc ctgcagggca tcgagacgct gccggtgcgc atggcgcgcc acgtcgagaa
     1981 cacccgcgcg atcgtggcct tcctgcgcga gcatccgttc gtctccggcg ttggctaccc
     2041 cgagttgacc acgcatcccg acaatgcgct ggcggcgcgg ctgctgccgc gcggcgcggg
     2101 cgccgtgttc agcttcaacc tgcgcggcac gcgcgagcag ggacgccggt tcatcgaggc
     2161 gctgcggctc ttctcgcatc ttgccaacgt cggcgacgcg aagtcgctcg tgatccaccc
     2221 ggcgtcgacg acgcatttcc ggatgtcgga cgaagacctg cgccgcgccg gcatcaccga
     2281 ggggacgatc cgcctttcga tcgggctcga ggatcgcgcc gacctgatcg acgatctgtc
     2341 gcgggcgctc aacgcggcga gcaaggcctg atgcgcgtcg acgttgcggg agcgtcggtc
     2401 tacgcgtaca gcggcacgcg cgcattcgtt cgcgggcggc cgagcgtcgt gttcgtgcac
     2461 ggtgcggcga acgaccacgg cgtcttcgcg taccagtcgc gttacttcgc gtggcatggc
     2521 atgaattgcg tcgccgtcga tcttcccggt cacggacgct cggcgggcga cgcgctcgcg
     2581 agcgtcgagg cactcgccga ctggctgcgc gacgtgttcg acgcgctagg cgtatccgac
     2641 gtcgacctcg tcggccattc gctcggctcg ctcgcggcac tcgaatgcgc gtcgcgccat
     2701 ggcgagcgcg tgcgccggct cgcgttgctc ggtccgtcgg tgccgatgcc ggtcagcgac
     2761 gatctgctgg ccgctgcctc acggaacgac cacgtcgcct acgagctcat caacggctgg
     2821 tcgtttgcgg cgggcagcca gctgggcggc aacccggtgc ctggcatgtg gatgctcggc
     2881 aatgcgatgc ggctcatgga gcgcacgcgc gatggcgtgc tgtcgaccga tctcattgca
     2941 tgcaaccggt acgcgaacgg cctcgaggca gcggcgcgcg ttcgctgccc gacgctcgtc
     3001 gtgctcggtg cgcgcgacat catggcgccg acgcgcaatg cgaaggcgct gatcgcgacg
     3061 ctagccgacg ccacgacggt gacgctgccc gagaccggtc attcgatgat ggccgagcgg
     3121 ccggatcagg tgctcgacgc attgcgccgg tttctgccgg tcaaccccgc cacgtgatcg
     3181 agcatccgat cgccggtgtc tgctccaccg gcgcgcgccc ggtgcgcgcg acctgcgcca
     3241 tcgcttcgta gagctcgcgt cgcgcagcgg gattcgacga gcggcccgat gcgtcgaggc
     3301 gtccgcggta ggcgagcgtc aggtcggcgt cgaagccgaa gaactcgggc gtgcatacgg
     3361 cgtcgtaggc gcgcgcgaca tcctgcgatt cgtcgacgac gtacggaaag ctgaatcggt
     3421 gctcggccgc gaatacgttc atgttgtcga acgagtcctc gggatacgtc gcagcatcgt
     3481 tgctcatgat cgcgatgctg ccgatgccga gcggcgcgag cgcgtcggcg tcgcgcgcga
     3541 tcttggccgc aatcgcccgg acgtacgggc aatggttcgc aaggaacatc acgagcaggc
     3601 ccttcggtcc acgtgccgta tcgggcgtgt ggcgccgccc gtcgatgccg ggcaggtcga
     3661 aatatgcggc tggccagccg agcgcgaccg gcgtcgtcat cgccgtgcct gtgcgattgc
     3721 aacccggctc atcggacttc gcatggcgct tcctccttca tcggtggccg aaccatggga
     3781 gaatcgtagc accggtcgac caagcgctcg cgagtgacgc cacgcatctt tgtcggtgac
     3841 gatgtcgcag cgctcgcgcc aggggtcgac tacgtgctgc cgcaggcggc ggcacgccac
     3901 gtcgcgcagg cgctgcgcat gcgcaccgga cagccgctga cgctcttcac cggcagcggc
     3961 ggcgaatacg cgacgaccgt tgcgcacatc gaccgccgcc aggtggtcac gcacgtcgag
     4021 cgacatgagg cggtcgagcg cgagagcccg catgcggtga cgctcgtgca gtcgctgatc
     4081 gcggccgaaa tgatggattt cgtcgtgcgc aaggcggtcg agctcggcgt ggcggcgatc
     4141 gtgccgctgc ttgccgcacg aagccaggcg atagccgcgg atcgcgccga gcgccgcgtt
     4201 gcgcactggc ggcaggtggc aatcgccgcg tgcgagcagt gcggccgcaa ccgcgtcccc
     4261 ggcgtcgcgg acatcgtgcc gttcggcgaa tgggccgagg cgatcgagcc ggcggcggcg
     4321 ctggcgattc tcgacttcga cgcgtcgcga tcgcttgctg cactggcgtc gacggcgccg
     4381 ccgcgcagcg ttgcggtcgg ccccgaaggc ggatttgcgg cagacgagtt gcgcgatgca
     4441 ggcgcgcgcg gagccattgg cgttcacctc ggccgtcgca tgctgcgtgc ggaaacggcg
     4501 gcgatcgccg cgctcgcgac gctgaattca atcgcaggcg acgcgcagtg accaaatcgg
     4561 ggtcagagcc gtaattattg cgaccggcac cctggcgcct ggcaacgcag ctgcgcgccg
     4621 caataattac ggctctgacc ccgatttcca gtgccggccg caataattac agctctgacc
     4681 ccaattggat tacgcctccg acgccggccg gtcgcccgcg ac
//
LOCUS       DASSVX010000183         8103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_440575, whole genome shotgun sequence.
ACCESSION   DASSVX010000183 DASSVX010000000
VERSION     DASSVX010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440575"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFO53_07775"
     CDS             <1..358
                     /locus_tag="VFO53_07775"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET8876211.1"
                     /translation="RGFIVGGPLAYRLGAGFVPIRKKGKLPAENFGEDYVLEYGRDRL
                     EVHCDALAHGERVLLVDDLIATGGTAEAATRLVRTAGALLIGCAFVIDLPDLGGRRRL
                     EALGYRVRALCQFSGH"
     gene            complement(370..798)
                     /locus_tag="VFO53_07780"
     CDS             complement(370..798)
                     /locus_tag="VFO53_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HET8876212.1"
                     /translation="MTSQAQGLILYVHGARDPRWAEPFERLRERIAAQAPHCIVTLAF
                     LEHMAPDLASAAVQLAAQGADAVRIVPMFFGRGGHLRDDFPRQLAAARIAVPAVTFVV
                     TEAAGESEQVEDALAEFALRAPAVAVKKVEADSENSSTNR"
     gene            973..1497
                     /locus_tag="VFO53_07785"
     CDS             973..1497
                     /locus_tag="VFO53_07785"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HET8876213.1"
                     /translation="MSRLIRNRTIVDDRYTLLREAASLADVPDGVPVIVPLALWKERR
                     AALIARGEAGVWLAPGDDPAALTDDVRRLPLIAIDFPHATDSRSHSHARVLRGRYAFA
                     NELRAIGDVRRDQFEDLSQSGFDTVAIAASPRAERALDDQPNFRCEDESSAAAPRLTR
                     QDRESPGAARFPCA"
     gene            1502..2266
                     /gene="cobA"
                     /locus_tag="VFO53_07790"
     CDS             1502..2266
                     /gene="cobA"
                     /locus_tag="VFO53_07790"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HET8876214.1"
                     /translation="MTGVVYLVGAGPGAADLLTLRAARLLARADIVFHDALVHPDTVA
                     LAAIAETVAVGKRCGRHSTAQRFINKRLIDAAAKYAVVVRLKGGDPMLFGRAQEEIAA
                     LEAAGVRYEIVPGVTAALAAAATIGTSLTQRGSVRSFALATPRVGDGQNGSDWITGFR
                     HADAGAIYMGRKEAGAIAASLIAAGMAPSTPVAIVVDASLSTAETHYSTLDRLPRLAA
                     REIHGAALLLIGPQFCSRHAHVGATALHEPPLAARA"
     gene            2316..2954
                     /locus_tag="VFO53_07795"
     CDS             2316..2954
                     /locus_tag="VFO53_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HET8876215.1"
                     /translation="MPAALRLPLFDLAPDSQRAWLVERLARPAPPAARGLSDGFRLPG
                     REGLSVPAAVLVPLVNRPQGLQLLLTQRSADLPDHPGQISFPGGRVEPGDADPAAAAL
                     RETGEEIGLAPGNVQVLGRLPSYDTVSGYCVQPVVGWIDPPFELAPDPVEVADVFEVP
                     LEFILDRSNHQRHFRMLGEMRRDYWAIPWLSRYIWGATAAMLLILDRTLRDE"
     gene            2951..3817
                     /locus_tag="VFO53_07800"
     CDS             2951..3817
                     /locus_tag="VFO53_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8876216.1"
                     /translation="MMLTKTAPRTCHFRGLSPHGFHHVVYYEWGARDNANVVVCVHGI
                     GRNGRDFDVLGESLAPTHRVVAIDMPGRGASEWLRDPMDYVTPVYLATLTALIAASGA
                     DEIAWVGTSMGALLGMIAASQPGTPVTRLVVNDAGPALEPAALARIGQYFGADPRFAT
                     YAELDAYVRHISAPFGPLTDVQWEHVVGTNARQHADGSWGLGYDPGIAVPFRALSAPP
                     DLWPQWDAIGCPTLVLRGAQSDLLSHATAMAMAARGPRARVAEIPGVGHAPMLLAADQ
                     VATVVEFLRSPV"
     gene            3870..5411
                     /locus_tag="VFO53_07805"
     CDS             3870..5411
                     /locus_tag="VFO53_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876217.1"
                     /translation="MSLALRLPPFAERPRDAPEIRPSRLASWLTETSTREPGFAARII
                     GEALAATNRVSMSHSRRLDLTEQYWKTAALLWPRLEHRFIRASHPLQGDDLEAAKAAL
                     MLANELSTSYKRLLAGEASRRLRFGGPRRLVALVRRAFQATSRVLSNSYLTYAPVPAH
                     TWYDAHEIYMFTRERKIHRHAVTIDQPDITLERLYTQSLLLALANPYGFLPSQLAVVL
                     HYLEENAQLAKLTPVPPVHRMAKAVAIVPVGHDFPPFSANKGGNAEGTKLFLLTFDLA
                     FELQEQIRAIESGAALPREAGSDADARIRHVALLKRLLRQWAIPPARQFNRLPSRAHV
                     AMCAGLSGVWHYTRGNEHRPPNGVPPPPPMTQCQVVNHTSGGYALRLIDPAAAPLRVG
                     DLIALRVEGREGLQVAMVRWFRNTLSGAVLEFGCELLSDAPEAAAAAAEHAPESLLMP
                     VLVLPEEQHDAEAPPPQLAAPAGAFALEQAVSLRRRGGSGFAVLTKLVEQGPGFDLYE
                     FVTVT"
     gene            5458..5580
                     /locus_tag="VFO53_07810"
     CDS             5458..5580
                     /locus_tag="VFO53_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876218.1"
                     /translation="MRPPANVAAQPHAWAIIPRVAVAATLALLLAWFRRAAARR"
     gene            complement(5577..6101)
                     /gene="lspA"
                     /locus_tag="VFO53_07815"
     CDS             complement(5577..6101)
                     /gene="lspA"
                     /locus_tag="VFO53_07815"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET8876219.1"
                     /translation="MTRATAAVTQRAPASANLRWLWLAVLVIVLDQLTKHLILASFRP
                     GEELPLTPFASLVLAFNRGAAFSFLADGGGWQRWFFALIAVAACAAMLWFLRRGGRAL
                     FCAGLALIIGGALGNLFDRLTLGHVVDFLLLHWRGWYYPAFNVADSAITIGAVALIID
                     SFRERRPREPMPRG"
     gene            complement(6098..>8103)
                     /locus_tag="VFO53_07820"
     CDS             complement(6098..>8103)
                     /locus_tag="VFO53_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET8876220.1"
                     /translation="DLAGACLARYGLDGSVVATAHGTSLERIAFRHPFYDRASPVYLG
                     DFVTLEQGTGIVHSSPAYGVDDFLSCRAYGMSDDDMLNPVQGNGRYAASLPYFGGLSI
                     WEANPRIVDKLRDVHALFHAEKFAHSYMHCWRHKTPIIYRATTQWFAGMDDVPGYRGV
                     KPARTLRETALRGIENTEFFPAWGKARLFGMIANRPDWTLSRQRQWGVPLPFFVDRET
                     EQLHPDTPALLELAATKVEADGIESWFEATSEDFGVDETRYRKLTDTLDVWFDSGTTH
                     QTVMGGPDGRQRRTGSHPADTGFPADLYLEGSDQHRGWFHSSLLTSCMLNGVPPYKAL
                     LTHGFVVDGQGRKMSKSKGNVVAPQKVAGTLGAEILRLWVGATDYSGELSISDEILKR
                     VVESYRRIRNTLRFLMANTSDFDAARDAVPQAELFEIDRFALAKARALMDAVAGDYGR
                     YEFHLVVQRLQTYCSEDLGGFYLDVLKDRLYTAGANSRARRSAQTALALIRDALLALM
                     APVLSFTAEEAWQLLHPGDPTIFVRTWAGMLPEAHDEALVAKWERILAVRALVQKELE
                     AVRQGGAIGSSLQADVDIVAGGDDYDDLASLGDDLRFVLITSAASVRRGEPFAIAVTP
                     SRYRKCERCWHWRADAGGDPSHPGLCARCIANLFGAGEPRQYA"
BASE COUNT         1245 a         2809 c         2751 g         1298 t
ORIGIN      
        1 gcgcggattc atcgtcggcg gtccgctcgc gtaccgcctc ggcgccgggt ttgttccgat
       61 tcgcaagaag ggcaagctgc cggccgaaaa cttcggcgag gactatgtgc tggagtacgg
      121 tcgcgaccgc cttgaagtgc attgcgacgc gctcgcgcac ggcgagcgcg tgctgcttgt
      181 cgacgatctc attgcgaccg gcggtaccgc ggaggcagca acgcggctcg tccgcacggc
      241 gggtgcgttg ctgatcggtt gcgcgttcgt catcgacctg ccggatctcg gcggccgccg
      301 caggctggag gcgctggggt accgggtgcg tgcactatgt cagttctcag gacattgagt
      361 ttatttatat cagcggtttg tggagctatt ctcagagtct gcttcaactt tctttacggc
      421 aacagccggc gcccgcaacg caaactcggc gagtgcgtcc tcgacctgct cgctctcgcc
      481 ggcggcctcc gtcacgacga atgtgacggc agggactgcg atgcgggccg ccgcgagctg
      541 tcgcgggaaa tcgtcacgca ggtgcccgcc gcggccgaag aacatcggca cgatgcgcac
      601 ggcgtcggcg ccctgcgccg cgagctgtac ggcagcactc gcaagatcgg gcgccatgtg
      661 ctcgagaaat gcgagtgtga cgatgcaatg cggcgcctgc gcggcgatgc gttcgcgtag
      721 ccgctcgaac ggctcggccc agcgtggatc gcgcgcgccg tgcacgtaca ggatcaatcc
      781 ctgcgcctgg ctcgtcatcc ggcgattata gggatgcggc cgcaacgatc gggcgggccc
      841 ggcccggtgg ttcatgcggc cgggcaataa gtgcatagca tctgattcct tctgttcgca
      901 cgcgatggcg ggtactgtcg tggggttgtc cgccgtgctt cggccggacg tccactctgc
      961 gaggacagcg ccatgtcacg tctcatccgg aatcgcacga tcgtcgacga ccggtacacg
     1021 ttgttgcgcg aagcggcgtc gctggccgat gtgcccgatg gcgttcccgt catcgtgccg
     1081 ctcgcgctct ggaaggagcg gcgcgccgcg ctcatcgcgc gcggcgaagc gggcgtctgg
     1141 ctcgcacccg gcgacgatcc cgccgcgttg accgacgacg tccgtcgcct gccgctcatc
     1201 gccatcgact tcccgcacgc caccgacagc cgcagccact cgcatgcgcg ggtgctgcgc
     1261 ggccggtatg cattcgcgaa cgagcttcgc gcgatcggcg acgtgcgccg cgaccagttc
     1321 gaggatctgt cgcaatcggg tttcgacacc gtcgcgatcg cggcttcgcc gcgcgccgaa
     1381 agagcgctcg acgatcagcc gaattttcgc tgcgaggatg agtcgagcgc cgcggctccc
     1441 cggctcaccc gccaggaccg cgaatcacca ggcgccgcgc ggttcccgtg cgcatgaagg
     1501 catgaccggc gtcgtctatc tggtcggcgc aggacccggc gcagcggatc tgctcacgct
     1561 ccgcgcggcg cggcttctcg cgcgcgccga catcgtgttc cacgatgcgc tggttcatcc
     1621 cgacacggtt gcgctcgcgg cgatcgccga aacggtcgcc gtgggcaagc gctgcgggcg
     1681 ccactccacg gcgcagcgct tcatcaacaa gcggctgatc gacgctgccg cgaaatacgc
     1741 ggtcgtggtc cggttgaagg gcggcgaccc gatgctgttc ggacgcgcgc aggaagagat
     1801 tgctgcgctc gaagcggcgg gcgtccgata cgagatcgtg cccggcgtca cagcggcgct
     1861 cgcggcagcg gcaacgatcg gaacctcgct cacgcagcgc ggcagcgttc gttcgttcgc
     1921 gctcgcgaca ccgcgggtgg gcgacggcca gaacggcagc gactggatca ccggcttccg
     1981 ccatgcggac gcgggcgcga tctacatggg gcgcaaggaa gcgggcgcga tcgctgcgtc
     2041 gctgattgct gcggggatgg caccgtcgac gccggtcgcg atcgtcgtcg acgcgtcgct
     2101 ttcgaccgca gagacgcatt actcgacgct tgatcgcctg cctcggttgg ccgcgcgcga
     2161 aatccacggc gcggcgctgc tgttgatcgg cccgcagttt tgttcgcgac acgcgcacgt
     2221 cggcgccacg gcattgcacg agcccccgct cgctgcacgc gcatgacgcg gtatcattcg
     2281 cgcacggccg caggtcccgg acggcccctc gcgcaatgcc cgcggctctc cgcctcccgc
     2341 ttttcgatct tgctcccgat tcgcagcgcg cctggctcgt cgagcggctg gcgcgacctg
     2401 cgccgcccgc cgcccgcggg ttgagcgacg gctttcgcct ccccggccgg gagggcttga
     2461 gcgtgccggc cgcggtgctc gttccgctcg tcaaccggcc gcagggcctg cagctgctcc
     2521 ttacgcagcg aagcgcggat ttgcccgatc atcccgggca gatcagcttc cccggcggcc
     2581 gcgtggagcc gggcgatgcc gatcctgccg cggcggcact gcgcgaaacc ggcgaggaga
     2641 tcggcctggc gccgggcaac gtgcaagtgc tcggtcgcct gccttcgtac gacaccgtca
     2701 gcgggtattg cgtgcagccg gtcgtcggct ggatcgatcc gccgttcgag ctcgcgcccg
     2761 atcccgtcga agtcgccgac gtgttcgagg tgccgctcga attcattctc gaccggtcga
     2821 atcatcagcg gcacttccgg atgctgggcg agatgcgccg cgactattgg gcaatcccgt
     2881 ggctttcgcg ttatatctgg ggcgcgacgg cggcgatgct gctgatcctc gatcgaacct
     2941 tgcgcgacga atgatgctga cgaaaacggc gccgcgcact tgccatttcc gcgggctctc
     3001 tccgcacgga ttccatcacg tcgtctacta cgagtgggga gcgcgcgaca acgcgaacgt
     3061 cgtcgtctgc gtgcacggca tcggacgcaa cggccgcgac ttcgacgtgc tcggcgaatc
     3121 gcttgcgccg acgcatcgcg tggtcgcgat cgacatgccg ggtcgcggcg ccagcgaatg
     3181 gctgcgcgat ccgatggact acgtcacccc cgtctatctc gccacgctga ccgcactgat
     3241 cgcggcgagc ggcgcggacg agatcgcctg ggtcggcacg tcgatgggtg cgctgctcgg
     3301 aatgattgcg gcctcgcaac ccggcacgcc ggtgacgcgc ctcgtcgtca acgacgcggg
     3361 tcctgcgctc gagccggcgg cgctcgcccg catcgggcag tacttcggcg ccgatccacg
     3421 cttcgccacg tatgccgagc tcgacgcgta cgtgcgtcac atctcggcgc cgttcggtcc
     3481 gctcaccgat gtgcaatggg agcatgtcgt gggcacgaac gcgcgccagc acgcggacgg
     3541 gagctggggt ctcggctacg acccggggat cgcggtgccg tttcgcgcgt tgtcggcgcc
     3601 gcccgatctg tggccgcaat gggacgcgat cggctgcccg acactcgtgc tccgcggcgc
     3661 gcaatccgat ctgctgtcgc acgcgaccgc aatggcaatg gccgcccgcg gcccgcgcgc
     3721 gcgtgttgcg gaaattcccg gtgtggggca tgcgccgatg ctgctcgcgg ccgaccaggt
     3781 cgcgaccgtc gtcgaattcc tccgttcgcc cgtataatcg tcgcgccgcc tccgcccccg
     3841 actgcattcc ggcgcaaccg tcagtctcca tgtcgctcgc tctccgactg ccgccgttcg
     3901 ccgaacgccc gcgcgacgct cccgaaatcc ggccgtcgcg cctcgcaagc tggctgaccg
     3961 aaacgtcgac gcgcgaaccg ggcttcgccg cgcgcatcat cggcgaggcg ctcgccgcga
     4021 ccaatcgcgt ttcgatgagt cattcgcggc gcctcgatct caccgagcag tactggaaga
     4081 ccgccgcgct cctgtggccg cgtctcgagc accgcttcat ccgggcctcg cacccgctgc
     4141 agggcgacga tctcgaggcc gcaaaggcgg cgttgatgct cgcgaacgag ctgtcgacgt
     4201 cgtacaagcg cctgctggcc ggggaggcgt cgcgaaggct gcgtttcggc ggaccgcgcc
     4261 gcctcgtagc actcgtgcgc cgggccttcc aggcgacgtc acgcgtgctt tcgaacagct
     4321 acctcacgta cgcgccggtg cccgcgcaca cgtggtacga cgcgcacgaa atctacatgt
     4381 tcacgcgcga gcgcaagatt catcggcacg cggtgacgat cgaccaaccc gacatcacgc
     4441 tcgagcggct gtatacgcag tcgctgctgc tcgcccttgc caatccgtac ggattcctgc
     4501 cgagccagct tgccgtggtg ctgcattacc tcgaggagaa cgcgcaactc gcgaagctga
     4561 cgccggtgcc gccggtgcac cggatggcga aggcggtggc gatcgtcccg gtcggccacg
     4621 actttccgcc gttctcggcg aacaagggcg gcaacgccga gggcacgaag ctgttcctgc
     4681 tcacgttcga ccttgccttc gagctgcagg aacagatccg tgcgatcgaa tcgggcgccg
     4741 ccctgccccg cgaagcgggc agcgacgccg atgcgcgcat acggcacgtc gcgctgttga
     4801 agcggctgct gcgccagtgg gcgattccgc cggcgcgcca gttcaaccgg ctgccgtcgc
     4861 gtgcgcacgt cgcgatgtgt gcggggctgt ccggcgtatg gcactacacc cggggcaacg
     4921 agcatcggcc gccgaacggc gttccgccgc cgcccccgat gacgcagtgc caggtcgtca
     4981 accatacgtc gggcggttat gcgctgcgcc tcatcgaccc ggcagcagcg ccgctgcgcg
     5041 ttggcgatct gatcgcattg cgcgtcgagg ggcgcgaggg actgcaggtc gcgatggtgc
     5101 gctggtttcg caacacgctg agcggcgccg ttctcgaatt cggctgcgag cttttgtccg
     5161 atgcacccga agcggcggcc gccgccgccg agcacgcgcc cgaaagcctg ctgatgccgg
     5221 tgctcgtgct tcccgaggag cagcacgatg cggaagcgcc tccgccgcaa cttgcagcac
     5281 ctgcgggcgc cttcgcgctc gagcaggcgg tgagcctgcg ccgccgcggc ggctcgggct
     5341 tcgccgtgct gacgaagctc gtcgagcaag ggccgggatt cgacctgtac gagttcgtga
     5401 ccgtcacgtg agcggacgcg tagactcgag cgccgaagcg ccgccccgag gcgcgtaatg
     5461 cgcccgccgg ccaacgtcgc ggcgcagccg catgcgtggg cgatcattcc acgcgtcgcc
     5521 gtcgccgcga cgctcgcgct gctacttgcc tggtttcgcc gggcggccgc tcgacgctag
     5581 cccctcggca tcggctcgcg cggacgccgc tcgcgaaagc tgtcgatgat cagcgcgacg
     5641 gcgccgatcg tgatcgcaga gtccgcaacg ttgaacgccg ggtagtacca gccgcgccag
     5701 tgcagcagca ggaaatcgac gacgtggccg agcgtcagcc gatcgaacag gttgccgagc
     5761 gcgccgccga tgatcagcgc gagacccgcg cagaacagcg cgcggccgcc gcggcgcagg
     5821 aaccacagca tcgccgcgca cgcggccacc gcgatcagcg caaagaacca gcgttgccag
     5881 ccgccgccat cggccaggaa gctgaacgcc gcgccgcgat tgaatgcgag gaccaggctt
     5941 gcgaacggcg tcagcggcaa ttcctcgccg gggcgaaacg acgcgaggat caggtgcttg
     6001 gtgagctggt cgagcacgat gaccagcacg gcaagccaga gccagcgcaa gttcgcggaa
     6061 gccggcgcgc gctgcgtcac ggcggcagtc gcccgcgtca cgcgtattgc cgcggctcgc
     6121 ccgcgccgaa gaggttcgcg atgcagcgtg cgcacaagcc gggatgcgaa gggtcgccgc
     6181 cggcatccgc gcgccagtgc cagcagcgct cgcatttgcg gtagcggctc ggcgtcaccg
     6241 caatggcgaa cggctcgccg cgacgcacgc tcgccgccga cgtgatgagc acgaagcgca
     6301 ggtcgtcgcc gagcgacgcc agatcgtcgt agtcgtcacc gccggcgacg atgtcgacgt
     6361 ccgcctgcag cgacgaaccg atcgccccgc cctgccgcac cgcctcgagc tccttttgca
     6421 cgagtgcgcg cacggcgagg atgcgctccc acttcgcgac gagcgcttcg tcgtgcgcct
     6481 cgggcagcat cccggcccac gtgcgcacga agatcgtcgg atcgccggga tgcagcaact
     6541 gccacgcttc ctcggcggtg aacgaaagca ccggcgccat cagcgcgagc agcgcgtcgc
     6601 ggatcagcgc gagcgccgtc tgcgcggagc ggcgcgcacg gctgtttgcg cccgccgtat
     6661 aaagccggtc cttcagcacg tcgaggtaga agccgccgag gtcctcggag cagtacgtct
     6721 gcaggcgctg cacgaccaga tggaactcgt agcggccgta gtcgccggca accgcgtcca
     6781 tcaacgcgcg cgccttcgcg agcgcgaagc ggtcgatctc gaagagctcg gcctgcggca
     6841 cggcatcgcg cgcggcgtcg aaatcggacg tattcgccat caggaagcgc agcgtgttgc
     6901 ggatgcgccg gtagctttcg acgacgcgct tcaggatctc gtcggagatc gacagctcgc
     6961 cggagtagtc ggtggcgccg acccacaggc gcaggatttc cgcccccagc gtgcccgcga
     7021 ccttttgcgg cgcgacgacg ttgcccttcg acttcgacat cttgcggcct tgaccatcga
     7081 cgacgaagcc gtgcgtgagc agcgccttgt acggcggcac gccgttcagc atgcacgatg
     7141 tcagcagcga cgaatgaaac cagccgcggt gctggtccga gccttcgaga tacaggtccg
     7201 cgggaaaacc ggtgtccgcc ggatgcgagc cggtgcgacg ctggcgacca tcgggcccgc
     7261 ccatcaccgt ctggtgcgtc gtgcccgaat cgaaccagac gtcgagcgtg tcggtgagct
     7321 tgcgatagcg cgtctcgtcg acgccgaaat cctcgctcgt cgcctcgaac cagctctcga
     7381 tgccgtcggc ttcgaccttc gtcgcggcga gctcgagcaa cgcgggcgtg tcgggatgca
     7441 actgctcggt ttcgcgatcg acgaagaacg gcagcggaac gccccattgc cgctgccgcg
     7501 acagcgtcca gtcgggccgg ttcgcgatca tgccgaagag gcgcgccttg ccccacgcgg
     7561 gaaagaactc ggtgttctcg atgccccgca gcgcggtttc acgcagtgtc cgcgcgggct
     7621 tcaccccgcg gtaccccgga acgtcatcca tcccggcgaa ccactgcgtc gtcgcgcgat
     7681 agatgatcgg cgtcttgtgg cgccagcagt gcatgtagct gtgcgcaaac ttttcggcgt
     7741 ggaacagcgc gtgcacgtcg cgcagcttgt cgacgatgcg cgggttggct tcccagatcg
     7801 acaagccgcc gaagtatggc aggctcgccg cgtagcggcc gttgccctgt accggattga
     7861 gcatgtcgtc gtcggacatc ccgtaggcgc ggcacgagag gaaatcgtcg acgccgtaag
     7921 ccggggagct gtggacgatg ccggtgccct gctcgagcgt gacgaagtcg ccgagataaa
     7981 ccggcgacgc gcgatcgtaa aacgggtgac gaaacgcgat gcgctcgagc gaggtgccgt
     8041 gtgccgtcgc gacgaccgaa ccgtcgagcc cgtagcgcgc aagacaggcg ccggcgagat
     8101 cct
//
LOCUS       DASSVX010000184         4351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_407452, whole genome shotgun sequence.
ACCESSION   DASSVX010000184 DASSVX010000000
VERSION     DASSVX010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407452"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VFO53_07825"
     CDS             complement(<1..1364)
                     /locus_tag="VFO53_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HET8876221.1"
                     /translation="MKREVIRTPGAPAPMAHYCQGVVAGNTIFAAGQIASDYKTGVAP
                     EARQDPAFPYYGSNIQKQARYILQNLRGVFEAAGCGLQDVVKSQVFMTDLNEFFHFDQ
                     VWKEFFPSPPPRTTVQMSGLLVPGCRVEIDLTAIQSSVERRVIRGASTPTPMAHYCQG
                     VLVDDTLYAAGQIASDYRTGVPPEARRDPAFPYYASDIERQTRYVLSNIRSVYEAAGC
                     DFSDTVKAQVFLTDLDDFVHFDRVWREFFPSPPPRTTVQVGALLVPGCRIEVDLTAVH
                     PRVPRKVIATKDAPAPMAHDCQGVLVGDMLYAAGQMASDQRTGVAHEARQDPAFPYYG
                     SDIQKQARYVLGNLRSVFEAAGCSLDDVVKSQVFLMDLDDFYYFDQVWKEFFPSPPPR
                     TTVQVSGLLVPGCRVEIDLTGVKPGVGRRVIRGASTPTPMAHYCQGVLVDDTLYAAGQ
                     IASD"
     gene            complement(1376..2077)
                     /locus_tag="VFO53_07830"
     CDS             complement(1376..2077)
                     /locus_tag="VFO53_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876222.1"
                     /translation="MLALDNVTLHRGHTRVLDSVSLTVDEGELVALIGANGAGKTTVL
                     RTISGILKPTSGVIMFRGKPIHTADSDAIVRLGIAHCPEERKIWPQMSVHEHLELGAF
                     IRSDSDVGADMQRIFDIFPVLKERREQLAGTLSGGQQQMLAIGRALMSRPSLVLFDEP
                     SLGLAPIMVETIGTMIAGIRDRGSTVLLVEQNASLALRLADRAYVLETGRVTLSGPAK
                     AMIENPEVHRAYLGG"
     gene            complement(2061..2795)
                     /locus_tag="VFO53_07835"
     CDS             complement(2061..2795)
                     /locus_tag="VFO53_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876223.1"
                     /translation="MPALLEGRGLSVRFGGLLALKGIDLAVDRGEVLSVIGPNGAGKT
                     TLFNVITGRVRPTTGSVVFRDRAIERLAPFRIAQLGVVRTFQKTEVFGELALIDGVAA
                     GALARNDPDPEARAREALAIVGLGSKAGLSAEQLSYGDQRLLEIAQALAADPAVLLLD
                     EPASGMNHEEAAHIMRLVVELRARGLAVVLVEHNMHVVMSISDRVVVINYGEKIAEGA
                     PADVRNDPRVIEAYLGVPQPADARAR"
     gene            complement(2779..3762)
                     /locus_tag="VFO53_07840"
     CDS             complement(2779..3762)
                     /locus_tag="VFO53_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8876224.1"
                     /translation="MPEPASYRVATTVVLVALCAVPLVVHDEYYLHLFIVSGVFVVAV
                     LGLDLIVGYVGQLSLAHAAFFAMGAYTSALLFVRLKWSMWLGLPAAALVAGLVALALG
                     SVILRTRGHRFIIITVVFAELMRLVATNWVDVTRGFMGLPGLTIPALEIPGVGTIDIA
                     AKARFYYVVLIAAIVAFVLCRLIVRSSIGRKFVLIRENEPLAESLGISAFRYSMIAFA
                     VGAALAGAAGSLYAHYVGFVSPDLFNFSYVTIMLIMVILGGKGTLAGPALGAVIFTFL
                     PELLREASHWRMIVFATILIVATLFMPKGIVFPLVTHLLPHQRRLRNARAP"
     gene            complement(3755..>4351)
                     /locus_tag="VFO53_07845"
     CDS             complement(3755..>4351)
                     /locus_tag="VFO53_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8876225.1"
                     /translation="EAPMMTTTLVTIGLSIFLENFALLLWGPQPQSIPTPFATKPLSF
                     LGVYTTLLYVFCMGVTVALIIVGHVLIRRTRMGKAMRATFQNKEAAALVGIRIDRIYA
                     YAFAFGAALAAAAGAVLGGFIPVEPATGGIVTLKAFIVVILGGMGSFVGAIVGGLVLG
                     LAEALGAGYMPSGWKDAIGFFAVILVLLFRPEGLFKRA"
BASE COUNT          812 a         1401 c         1478 g          660 t
ORIGIN      
        1 tagtcggacg cgatctgtcc cgccgcatag agcgtgtcat cgacgagcac gccctggcag
       61 taatgcgcca tcggtgtcgg cgtcgatgca ccgcggatca cgcgccgtcc gacgccgggt
      121 ttcacgccgg tgaggtcgat ctcgacgcgg cagccgggaa cgagcagtcc gctcacctgc
      181 accgtggtgc gcggcggcgg tgacgggaag aactccttcc acacctggtc gaagtagtag
      241 aaatcgtcga gatccatcag gaacacctgc gatttcacca cgtcgtcgag cgagcatccg
      301 gcagcctcga acacgctgcg caggttgccg agaacgtagc gcgcctgctt ctggatgtcc
      361 gagccgtaat acggaaacgc cgggtcctgg cgagcttcgt gcgccacgcc ggtgcgctga
      421 tccgatgcca tctggcccgc cgcgtagagc atgtcgccga cgagcacgcc ctggcagtcg
      481 tgcgccatcg gcgccggcgc gtccttcgtc gcaatcacct tgcgcggcac gcgcgggtgc
      541 accgccgtga ggtccacttc gatccggcat ccgggcacca ggagcgcacc gacctgcacc
      601 gtcgtgcgcg gcggcggcga cggaaagaat tcgcgccata cgcgatcgaa gtgcacgaag
      661 tcgtcgagat cggtgaggaa gacctgcgcc ttgacggtgt cggagaaatc gcagccggcg
      721 gcttcgtaca ccgagcggat gttcgaaagc acgtagcgcg tttgccgctc gatgtccgat
      781 gcgtaatacg ggaacgccgg gtcgcggcgt gcctcgggcg gaacgccggt gcggtagtcg
      841 gatgcgatct gtccggccgc atagagcgtg tcgtcgacga gcacgccctg gcagtaatgc
      901 gccatcggcg tcggcgtcga cgcaccgcgg atcacccggc gttcgacgct ggattggatg
      961 gccgtgaggt cgatctcgac ccggcagccg ggaacgagca gtccgctcat ctgcaccgtg
     1021 gtgcgcggcg gcggtgatgg aaaaaactcc ttccatacct ggtcgaaatg gaagaattcg
     1081 ttcaggtccg tcatgaacac ctgcgacttc acgacgtcct gcaagccgca acccgcggcc
     1141 tcgaacacgc cgcgcaggtt ctgcaggatg tagcgcgcct gcttctggat gttcgagccg
     1201 taatacggga atgccggatc ctgtcgtgcc tcgggggcga caccggtctt gtagtcggag
     1261 gcaatctgcc ccgcggcgaa gatcgtgttg ccggcgacga cgccctggca gtaatgcgcc
     1321 atcggtgccg gcgcgcccgg tgtcctgatc acttcacgct tcatcctggt tcctcctatc
     1381 ctccgagata cgcccggtga acctcggggt tctcgatcat ggccttcgca ggtccggaaa
     1441 gtgtcacgcg gccggtttcg agcacgtacg cccgatcggc gaggcgcaat gcgagcgacg
     1501 cattctgctc gacgagcagg acggtgctgc cgcggtcgcg aatgcccgcg atcatcgtgc
     1561 cgatcgtctc gaccatgatc ggcgcgagtc cgagcgacgg ctcgtcgaag agaacgagcg
     1621 acggccgcga catcagcgct cggccgatcg cgagcatctg ctgctgacct ccggagagcg
     1681 tgccggcgag ctgctcgcgg cgctccttca gcaccgggaa tatgtcgaag atgcgctgca
     1741 tgtcggcgcc gacatcgctg tcgctgcgaa tgaacgcgcc aagctcgagg tgctcgtgca
     1801 cgctcatctg cggccagatc ttgcgttcct cggggcagtg cgcgatgccg aggcgcacga
     1861 tcgcgtcgga gtcggcggtg tggatcggct tgccgcggaa catgatgacg ccgctcgtgg
     1921 gcttcaggat gcccgagatc gtccgcagca cggtcgtctt gcctgcgccg ttcgccccga
     1981 tcagcgccac gagctcgcct tcgtccacgg tgagcgagac gctgtcgagc acgcgcgtgt
     2041 gtccgcggtg cagcgtgaca ttatcgagcg cgagcatcgg cgggttgcgg cacaccgagg
     2101 taggcctcga tgacgcgcgg atcgtttcgc acgtcggccg gggcgccttc ggcgatcttc
     2161 tcgccgtaat tgatcacgac cacgcgatcg gagatcgaca tgaccacgtg catgttgtgc
     2221 tcgaccagga caaccgcgag accgcgtgca cggagctcga cgacgagccg catgatatgc
     2281 gcagcctcct cgtgattcat cccggacgcc ggctcgtcga gcagcagcac ggccgggtcg
     2341 gccgcgagcg cctgcgcgat ttcgagcagg cgctgatcgc cgtaggaaag ctgctcggcg
     2401 gacaaaccgg ccttgctgcc gagaccgacg attgcaagag cctcgcgggc acgggcttcc
     2461 ggatcggggt cgtttcgcgc gagggcgccc gccgccacgc cgtcgatcaa tgcgagctcg
     2521 ccgaagacct cggtcttctg gaacgtacgc acgacgccga gttgcgcgat gcgaaagggc
     2581 gcgaggcgtt cgatggcgcg atcgcggaac acgacgcttc cggtggtcgg ccgcacgcga
     2641 ccggtgatga cgttgaagag ggtggtcttg cccgcgccgt tggggccgat cacgctcaac
     2701 acttcgccgc ggtccacggc gagatcgatg cccttcagcg cgagcaagcc gccgaagcgc
     2761 acgctgagcc cgcggccctc aaggagcgcg ggcattgcgc aggcgccgtt ggtgcggcag
     2821 aagatgggtg accagcggaa agacgatgcc cttcggcatg aacagcgtcg cgacgatcag
     2881 gatcgtggcg aacacgatca ttcgccagtg cgaagcctcg cgcagaagtt cgggcaggaa
     2941 cgtgaagatg accgcgccga gcgcgggccc ggcgagcgtg cccttgccgc cgagaatgac
     3001 catgatcaac atgatcgtca cgtaggaaaa attgaagagg tccgggctca cgaatcccac
     3061 gtaatgcgcg tacaggctgc ccgccgcgcc ggcgagcgcc gcgccgacgg cgaacgcgat
     3121 catcgaatag cgaaacgccg agatgccgag cgattcggcc agcggctcgt tttcgcggat
     3181 cagcacgaac ttgcggccga tcgacgagcg cacgatcagg cggcacagca cgaacgcgac
     3241 gatcgcggcg atcagcacga cgtaatagaa gcgcgccttc gccgcgatgt cgatcgtgcc
     3301 gacgccgggg atttcgaggg cagggatcgt gagccccggc agacccatga agccgcgcgt
     3361 cacgtcgacc cagttcgtgg cgacgagcct catcaactcg gcaaagacca cggtgatgat
     3421 gatgaagcgg tgtccgcgcg tcctgaggat caccgagccg agcgcgagcg cgacgaggcc
     3481 cgcgacgagc gcggcggcgg gcaatccgag ccacatcgac cacttgaggc gaacgaagag
     3541 cagcgccgac gtgtaagcac ccatcgcgaa gaacgccgcg tgggcgagcg aaagctgccc
     3601 gacgtagccg acgatgagat cgaggccgag caccgcgacg acgaagacgc ccgacacgat
     3661 gaagaggtgc aggtaatact cgtcgtgcac gacgagcggc accgcgcaaa gcgcgacgag
     3721 gacgacggtc gtcgccacgc ggtacgacgc gggttcaggc acgcttgaag aggccttcgg
     3781 gccggaacag caggacgagg atcaccgcga aaaagccgat cgcgtccttc cacccgctcg
     3841 gcatgtaacc cgcgccgagc gcctcggcga ggccgagcac gaggccgccg acgatcgcgc
     3901 cgacgaagct gcccatgccg ccgaggatca cgacgatgaa cgccttgagg gtcacgatgc
     3961 caccggtggc gggctcgacc ggaatgaagc cgccgagaac cgcaccggcg gccgcggcga
     4021 gcgccgcacc gaacgcgaag gcgtaggcgt agatgcggtc gatgcgaatg ccgacgagtg
     4081 ccgcggcctc cttgttctgg aacgtcgcgc gcatcgcctt gcccatgcgc gtgcgccgga
     4141 tcagcacgtg cccgacgatg atcagcgcga cggtgacgcc catgcagaaa acgtacagca
     4201 gcgtcgtgta aacaccgagg aagctcagcg gtttcgtcgc aaagggcgtc ggtatggact
     4261 ggggctgcgg cccccaaagc agcagcgcga aattctcgag gaagatcgag agcccgatcg
     4321 tgacgagcgt cgtcgtcatc atcggcgctt c
//
LOCUS       DASSVX010000185         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1557540, whole genome shotgun sequence.
ACCESSION   DASSVX010000185 DASSVX010000000
VERSION     DASSVX010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557540"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..417)
                     /locus_tag="VFO53_07850"
     CDS             complement(49..417)
                     /locus_tag="VFO53_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HET8876226.1"
                     /translation="MKFHLQAPTGNIVTGSGPGWVRVGGDEYRDNIVLRADGVEPGFA
                     PAGFDNLTAEDFAALLRSTPEIVLLGTGATQRFPRPAVTAPLHDARVGLEVMDTRAAC
                     RTYNILVAEGRSVTAALIVA"
     gene            514..1713
                     /gene="alaC"
                     /locus_tag="VFO53_07855"
     CDS             514..1713
                     /gene="alaC"
                     /locus_tag="VFO53_07855"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003161371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HET8876227.1"
                     /translation="MSSNPNAQFARIERLPPYVFSITSELKMEARRRGEDIVDFGMGN
                     PDGPTPAHIVSKLVETVQRGDTHGYSVSKGIPRLRRAICHWYDRRWGVSFDPETEAIV
                     TIGSKEGIAHLALATLGRGDTVLVPNPSYPIHIYGPVIAGADIRQVRLTPDVDFFAEL
                     EQTIKLSVPKPKMLIINFPANPTAQCVELPFFEKIIALAKEYGIWVVHDLAYADICFD
                     GWRAPSIMQVPGARDVAVEFFTMSKSYNMAGWRIGFMVGNRDLVHALSRIKGYHDYGT
                     FTPLQVAAIAALEGPQDCVAEITQRYERRRDVLVKGLHEAGWEVDVPKASMYVWARIP
                     EAYASLGSLEFAKHVLAKAKVSVSPGIGFGEYGDAHVRFAMIENEARTRQAIRGIKQM
                     FREDGLA"
     gene            1790..2884
                     /locus_tag="VFO53_07860"
     CDS             1790..2884
                     /locus_tag="VFO53_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876228.1"
                     /translation="MGFLLRPHALLRLVLLLLVPAVARADFESLPFALRLFLSVDRQS
                     NYSSTLARQASIAAFDGGSANPGAVAWRVPLEPATVVTGSFVDAPSTGGRTVIAAPVS
                     LRWQVLSAGTIALAYAFTQTLHAGGDNGLTQSLRSDEWIGGYGRRLDEHSALGFTARL
                     TSGTIVNDASTPALGGALSRNSTRFLAPDVALGYARDIGKALTLGLVGSYGVARGHTT
                     VTNVAPLLVPLPALGTTLALPPDTVLALVDDTISTVTLGAGFGLHPDDATALYFDAKA
                     LRLSTHHSGSQNLGRFAWGAEHTMHNGLIVRGGVGLDTIGKVNVSAGLGYRPTPSFEA
                     QFAVQTNAAPELNPELGRTRLLSASLAWIF"
BASE COUNT          484 a          957 c          983 g          530 t
ORIGIN      
        1 agctgtaatt attcgcatcc gcgaataatt acagctctga ccccgatctc acgcgacgat
       61 cagcgccgcc gtgaccgagc ggccctcggc gacgagaatg ttgtaggtcc ggcacgcggc
      121 gcgcgtgtcc atgacctcga gcccgacgcg cgcatcgtgc agcggcgcag tcacggcggg
      181 gcgtggaaag cgttgcgtcg cgcccgtgcc gagcagcacg atttccggcg tcgagcgcag
      241 caatgccgcg aagtcctcgg ccgtgagatt gtcgaaccct gccggggcaa agcccggctc
      301 gacgccgtca gcgcgcagga cgatattgtc gcggtactcg tcgccgccca cgcgcaccca
      361 tccgggtccg ctgccggtga cgatgttccc ggtgggcgcc tgcagatgaa acttcatcga
      421 ttgtgcattg cggtaagcgc tcgatttcgc ttacgattgt agtctttccc gatctttcgt
      481 cgctcggacg gctccgggcg cttacgacaa gacatgtcct cgaatcccaa cgcacagttt
      541 gcccgcatcg agcggcttcc gccgtacgtc ttcagcatca cgtccgagct caagatggag
      601 gcccgccgcc gcggcgagga catcgtcgac ttcggcatgg gcaacccgga cgggccgacg
      661 ccggcgcaca tcgtgagcaa gctcgtggaa acggtgcagc gcggcgacac gcacggctat
      721 tccgtgtcga aaggcatccc gcggctgcgc cgcgccatct gccactggta cgaccggcgc
      781 tggggcgtct cgttcgaccc ggagaccgaa gcgatcgtca cgatcgggtc gaaggagggc
      841 atcgcgcatc tggcactcgc gacgctcggc cgcggcgaca ccgtgctcgt gcccaatccg
      901 agctacccga tccacatcta cggcccggtg atcgcaggcg ccgacatccg gcaggtgcgg
      961 ctcacgccgg atgtcgattt cttcgccgag ctcgagcaga cgatcaagct gtcggtgccg
     1021 aagccgaaga tgctgatcat caatttcccg gcgaatccga cggcgcaatg cgtcgagctg
     1081 ccgtttttcg agaagatcat cgcgctcgcg aaggaatacg gcatctgggt cgtgcacgac
     1141 ttggcctatg ccgacatctg cttcgacggc tggcgggcgc cgtcgatcat gcaggtgcct
     1201 ggcgcgcgcg acgtggcggt cgagttcttc acgatgtcga agagctacaa catggcgggg
     1261 tggcgcatcg gcttcatggt cggcaaccgc gacctcgtcc atgcgctgtc gcggatcaag
     1321 ggctaccacg attacggcac gttcacgccg cttcaggtgg cggccattgc cgcgctcgaa
     1381 ggtccgcagg attgcgtcgc ggagatcacg cagcgttacg agcggcgtcg cgacgtgctc
     1441 gtgaagggcc tgcacgaagc gggctgggag gtcgacgtgc cgaaggcgtc gatgtacgtg
     1501 tgggccagga ttcccgaagc gtacgcgtcg ctcggctcgc tggaattcgc gaagcacgtg
     1561 ctggcgaagg cgaaggtgtc ggtgtcaccg ggcatcggct tcggcgaata cggcgatgcg
     1621 cacgtgcgct tcgcgatgat cgagaacgaa gcgcgcacgc gacaggcgat tcgcggcatc
     1681 aagcagatgt tcagggaaga tggcctggcg tgatgacgtg gcagggcgag ggcgcgcgcg
     1741 agcttgacgc acgtttcgtc gacgcaacga agaaggggag taccgcgaca tgggctttct
     1801 gcttcgtccg catgcactcc tgcgcctcgt gttgctgctg ctcgttcccg cggtagcgcg
     1861 cgccgacttc gaatcgctgc cgttcgcgct gcgcctgttc ctttcggtcg accgtcaaag
     1921 caattactcg tcgacgctcg cgcgacaggc gtcgatcgcc gctttcgacg gtgggtcggc
     1981 caaccccggc gccgtggcgt ggagagtgcc gctggaacct gctaccgtgg tgaccggcag
     2041 tttcgtcgat gcgccgtcga ccggcggacg cacggtgatc gcggcgcccg tttcgctgcg
     2101 ctggcaggtg ctaagcgcag gaacgatcgc tctcgcgtat gcatttaccc agacgctgca
     2161 cgccggggga gacaatgggc tcacgcagtc gctgcgctcg gacgaatgga tcggcggcta
     2221 cggacgccgg ctggacgaac attccgcgct tggtttcacc gcccggctga caagcggcac
     2281 gatagtgaac gacgcgtcga ccccggcgct cggcggcgcg ctctcccgca actcgacgcg
     2341 cttcctggcg cccgacgttg cgctgggcta tgcgcgcgac atcgggaagg ccttaacgtt
     2401 gggccttgtc ggaagttatg gcgtggcgcg cggccatacg acggtgacga acgtcgcgcc
     2461 gctgctcgtt ccgcttccgg ccctcggcac cacgctggcg cttccgcccg ataccgttct
     2521 cgcgcttgtc gacgacacga tttccacggt cacgctcggc gcgggtttcg gactgcatcc
     2581 ggacgacgcc acggcgcttt acttcgacgc gaaggcgttg cgcttgtcga cacaccactc
     2641 gggttcgcaa aacctcggtc gattcgcatg gggtgccgag cacacgatgc acaacggcct
     2701 gatcgtgcgc ggcggcgtcg ggctcgacac gatcggcaag gtgaacgtga gcgcgggcct
     2761 tggttatcgg ccgacacctt cattcgaagc ccaattcgcc gtgcagacga acgccgcgcc
     2821 ggagctcaac ccggaactgg gccgcactcg attgctgtcc gcctcgctgg cgtggatctt
     2881 ctgagcgcac cggcgatggt tacccgcgac gcggtgttgg cgtgctttcg gccggtcgtt
     2941 cacggacgtc cgaa
//
LOCUS       DASSVX010000186         7298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1123971, whole genome shotgun sequence.
ACCESSION   DASSVX010000186 DASSVX010000000
VERSION     DASSVX010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123971"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFO53_07865"
     CDS             complement(<1..450)
                     /locus_tag="VFO53_07865"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HET8876229.1"
                     /translation="MRLILIGPPGAGKGTQAAFIRAAYGIPQIATGDMLRAAVNAGTP
                     LGLAAKQVIDRGVLVSDDIIIGLVKDRLRAPDCARGYLFDGFPRTIPQADALKHAGIP
                     LDYVIEIDVPDAAIVERMSGRRVLVPSGRSNHVKFNPPKIRGHDDVTG"
     gene            complement(441..1238)
                     /gene="kdsB"
                     /locus_tag="VFO53_07870"
     CDS             complement(441..1238)
                     /gene="kdsB"
                     /locus_tag="VFO53_07870"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HET8876230.1"
                     /translation="MRFTVIVPARYASSRLPGKPLADLGGKPLVVRVAERARASGAER
                     VVVATDDVRIEEAARAWGVDAIMTRAGHPSGTDRLAEAAVALGLGDDEIVVNVQGDEP
                     LLDPALMRRVANTLAARPDAAIATACHPITQIDDVLNSNVVKVVLDARGNALYFSRAP
                     IPWARDAFSAEPPVLPAGLPIHRHYGLYAYRVAFLRTFPALAPAPIETHEALEQLRAL
                     WHGLRIVVDIAHGAPSPGIDTPEDLARVRGLPEFAAPATQPECSRCD"
     gene            complement(1265..1456)
                     /locus_tag="VFO53_07875"
     CDS             complement(1265..1456)
                     /locus_tag="VFO53_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET8876231.1"
                     /translation="MDRKLLEMLVCPVTKGPLVHDAERGELISRSARLAYPIRDGIPI
                     MLEEEARKLTPEEWEAGKE"
     gene            complement(1395..2459)
                     /gene="lpxK"
                     /locus_tag="VFO53_07880"
     CDS             complement(1395..2459)
                     /gene="lpxK"
                     /locus_tag="VFO53_07880"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HET8876232.1"
                     /translation="MTRAEAALARAWYSRRPTFVATLLRPLSWIFGAVAALRRAAYRA
                     KLLPSTRVPVPVVVVGNITVGGAGKTPLVAALAEALAARGRHPGVLSRGYGRRTRDVR
                     EAGAGDDPRDVGDEPLLLAAAGFPLVVGSDRAAAAQSLLRAHPEVDVLLSDDGLQHYA
                     LARDVEIAVVDAARGLGNGLLLPAGPLRETASRLHSVDALVWRTAPGQRAPMRGHPHE
                     FVAPYEPRPWVNVLDSTLPFDEGLLADPASVAIAGIADPASFFDALRARGFRGETRAF
                     ADHHAYARDDVAFVRAPAILMTQKDAVKCRAFADARMWMLPIRARVEPALVDFVVEKI
                     DGPKAPRNAGLPGHQGSPRS"
     gene            complement(2456..2890)
                     /locus_tag="VFO53_07885"
     CDS             complement(2456..2890)
                     /locus_tag="VFO53_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET8876233.1"
                     /translation="MNLRGNREREDPEINFIPLIDVLLVILIFLMVTTTYQRIAELSI
                     TLPEANADPAKDRPREINVGLDAQGRYKIGREPAFTFTTVDALAATLKRAAGDAKEPI
                     VVINADAQATHQSVIHVMEAARNAGLTHITFATQASPAAASR"
     gene            complement(2925..3530)
                     /locus_tag="VFO53_07890"
     CDS             complement(2925..3530)
                     /locus_tag="VFO53_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET8876234.1"
                     /translation="MWAMIQAAGWPIWPLIIASVVALALIFERLWSLRQSIVAPAGMV
                     DHVLVEFRHTGPTHDLLARTAEQGPLGRILAAGLANVKSPRPVMKEAIEEVGRVVTHD
                     LERFLTTLGTIASMAPLLGLFGTVIGMIEIFGSQSAAGSNPVQLAHGISVALYNTAMG
                     LIVAIPSMMFYRYFRSRVDALVVEMEQQAIKLVDFAHNERK"
     gene            complement(3617..4450)
                     /locus_tag="VFO53_07895"
     CDS             complement(3617..4450)
                     /locus_tag="VFO53_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HET8876235.1"
                     /translation="MNPFRFLFRVASRLRDIDLARAASSLSFTTLLAIVPLVTVAFAF
                     VARFPIFEDWLNVLEHFLLRHLLPFAGAAQVRAHIIGFAEQAGRLRGVSIAFIAVSAV
                     LAVTTVERELNAIWGIRRGRSLPKRMLVYAIGLTAGPVLLGASISITTWLVVHSLAVV
                     PFRRTFGAQVVEALPFVFSTAGLTLLYKWLPARHVPLVPALVAAAIGAAVFEAAKHAF
                     AWYLTRISSYEAVYGALAALPIFLLWVYFCWLIILAGAAVSATIAEPGGRRGRRGATS
                     G"
     gene            4575..5129
                     /gene="msrA"
                     /locus_tag="VFO53_07900"
     CDS             4575..5129
                     /gene="msrA"
                     /locus_tag="VFO53_07900"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET8876236.1"
                     /translation="MPNDPPVETALLGGGCFWCLDAVFRELAGVTSIESGYAGGHAPH
                     PSYEAVCSGDTGHAEVVRVSFDPAVLSYRDLLRVFFTIHDPTTRNRQGNDVGTQYRSV
                     IFALTDAQRADAKAVIGELDAQHVWPDPIVTDVAGPATFYPAEAYHQDYFERNGGQPY
                     CAYVVAPKVAKFRKAYAERLRKRA"
     gene            complement(5149..6303)
                     /locus_tag="VFO53_07905"
     CDS             complement(5149..6303)
                     /locus_tag="VFO53_07905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8876237.1"
                     /translation="MTQRFRVSPFGVLIALGVANHLVLTGSRVAVSLNALALGENAAT
                     VGGLMALFALFPMFLAIPAGRLADRIGVRGPMLAGSIGTAAGVLVVTLLPGLPALFAT
                     AMLTGVSFMLFQVAAQYATGEMGGVAARSRNFGLLALGYSTSSIAGPMIAGLMIDHAG
                     YRATFALLTLLPLLPIAVLATNRIALPGAHPARAARIDGSALDFVSHREMRRVFIING
                     LVSMAWELHTLFVPIYGHTIGLSASRIGLILAAFAAATFTVRLAMPAIASRISEHAVL
                     TSALYIGALVYLALPFSRTAGTLMGLSFCLGLGLGASQPMVMSLLHSHAPPGRMGEAA
                     GVRLSLVNSISVAVPLVFGAVGGVVGLSPVLWSVGVFLATGGWLTRAPRR"
     gene            complement(6300..6947)
                     /gene="pdxH"
                     /locus_tag="VFO53_07910"
     CDS             complement(6300..6947)
                     /gene="pdxH"
                     /locus_tag="VFO53_07910"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET8876238.1"
                     /translation="MPTPATDLAQLRVDYKRAALSERDAAADPYRQFARWFDEALAAA
                     VPEPNAMTLATVDPAGQPAARIVLLKAVDARGFVFHTNYDSRKGRDVAANARVALLFF
                     WPELERQVRVEGRAAQVDANESDAYFAQRPHGSQLAAWASPQSAPVADRQALEARFSA
                     VEGRFAAHVPRPANWGGMRVAADRFEFWQGRPSRLHDRLVWSRTDGGWSIGRLAP"
BASE COUNT         1260 a         2485 c         2551 g         1002 t
ORIGIN      
        1 gcccgtgacg tcgtcgtggc cgcggatctt cggcggattg aacttgacgt ggttcgagcg
       61 ccccgacggc acgagcacgc gccgtccgct catccgctcg acgatcgccg cgtccgggac
      121 gtcgatctcg atcacgtaat cgagcgggat gcccgcgtgc ttcagcgcat cggcctgcgg
      181 gatcgtgcgc ggaaagccgt cgaacagata gccccgcgca caatcgggcg cgcgtagccg
      241 gtccttgacg aggccgatga tgatgtcgtc ggagacgagc acgccgcgat cgatcacctg
      301 cttcgccgcg agcccgagcg gcgtgccggc attgacggca gcgcgcagca tgtcgccggt
      361 cgcaatctgc gggatgccgt aggccgcgcg tatgaacgcc gcctgcgtac ccttgcctgc
      421 gcccggaggc ccgatcagga tcagtcgcat cggctgcact ccggttgcgt cgcgggtgct
      481 gcgaattccg gaagcccgcg cacccgcgcg agatcctccg gcgtgtcgat gcccggtgac
      541 ggcgctccgt gcgcgatgtc gacgacgatg cgcaggccgt gccacagcgc gcgcaattgc
      601 tcgagtgctt cgtgcgtctc gatcggcgcg ggtgccagcg ccgggaacgt gcgcaggaac
      661 gcgacgcggt acgcgtacag cccgtaatgg cggtggatcg gcaatccggc cgggagcacc
      721 ggaggctcgg ccgagaacgc gtcgcgcgcc caggggatcg gcgcgcggct gaaatagagc
      781 gcattgccgc gcgcatcgag cacgaccttc accacgttcg aattgagcac gtcgtcgatc
      841 tgcgtgatcg gatggcacgc ggtggcgatc gccgcatcgg gacgcgcggc aagcgtgttc
      901 gccacacggc gcatcagcgc ggggtcgagc agcggctcgt cgccctgcac gttgacgacg
      961 atctcgtcgt cgccaagccc gagcgccact gcggcttcgg cgagccggtc ggtgccgctc
     1021 gggtgaccgg cacgggtcat gatcgcgtcg acgccccacg cacgcgcggc ctcctcgata
     1081 cgcacgtcgt cggtcgcgac gacgacccgc tcggcaccgc ttgcgcgcgc acgctcggcg
     1141 acgcgcacga cgagcggctt gccgccgaga tcggcgagcg gcttccccgg caggcgcgac
     1201 gatgcatagc gtgcggggac gatgaccgtg aagcgcacgc cggccgcgtc gtgcgccggc
     1261 cgcgtcactc cttccccgct tcccattcct cgggagtgag cttgcgcgcc tcctcctcga
     1321 gcatgatcgg aatgccgtcg cggatcggat aggcgaggcg cgccgagcgc gagatgagct
     1381 cgccgcgctc ggcatcatga acgaggggac ccttggtgac cgggcagacc agcatttcga
     1441 ggagctttcg gtccatcgat cttctccacg acgaaatcga ccagcgccgg ttcgacgcgc
     1501 gcgcgaatcg gcagcatcca catccgcgcg tcggcgaatg cccggcattt taccgcgtcc
     1561 ttttgcgtca tcaggatcgc cggtgcgcgc acgaaggcca cgtcgtcgcg cgcataggcg
     1621 tgatgatcgg cgaacgctcg cgtctcgccg cggaagcccc gcgcgcgcag cgcgtcgaag
     1681 aacgatgccg gatcggcgat ccctgcgatg gcgacgctgg cgggatccgc gagcagcccc
     1741 tcgtcgaacg gcagcgtgga atcgagcacg ttgacccagg gccggggctc gtacggcgca
     1801 acgaattcat gcggatgtcc gcgcatcggg gctcgctgtc cgggcgcggt tcgccacacg
     1861 agtgcatcga ccgagtgaag ccgcgacgcc gtctcgcgca acggacccgc cggcagcagc
     1921 agcccgttgc cgagcccgcg ggcggcatcg acgacggcga tctcgacgtc gcgtgcgagt
     1981 gcatagtgct gcagcccgtc gtcgctgaga agcacgtcga cctcgggatg cgcgcggagc
     2041 agcgactgcg ccgccgcggc gcggtcactc ccgacgacca gcggaaaccc ggcggcggcg
     2101 aggagcaacg gctcgtcgcc gacgtcgcgc ggatcgtcgc cggcgcctgc ttcgcgcacg
     2161 tcgcgcgtgc gccgcccgta gccgcggctc aacaccccgg gatggcgccc gcgcgcggca
     2221 agtgcttcag cgagtgcggc aacgagcggc gtcttgcccg cgccgccgac ggtgatgttg
     2281 ccgaccacga ccaccggcac cggaacgcgc gtggaaggca agagctttgc gcggtaggcg
     2341 gcacggcgca gggccgcgac agcgccgaat atccaggaca gcggccgcaa cagcgtcgcc
     2401 acgaacgtcg gccggcgcga ataccaggcg cgcgcgagcg ccgcctcggc gcgcgtcacc
     2461 gggaggccgc ggccggggat gcctgtgtcg cgaacgtgat gtgcgtcaag cccgcgttgc
     2521 gcgcggcttc catcacgtgg atcaccgatt gatgcgtcgc ctgcgcatcc gcgttgatga
     2581 cgacgatcgg ctccttcgcg tcgcccgcgg cgcgcttcag cgtcgcggcc agcgcatcga
     2641 cggtcgtgaa cgtgaacgcg ggctcgcggc cgatcttgta gcggccctgc gcatcgaggc
     2701 cgacgttgat ctcgcgcgga cgatccttcg ccggatcggc gttggcctcg ggaagcgtga
     2761 tcgaaagctc ggcgatgcgc tggtaggtgg tcgtgaccat caggaagatg aggatcacga
     2821 gcagcacgtc gatcagcggg atgaaattga tctccgggtc ctcgcgctcc cgattgccgc
     2881 gcagattcat cgcactgccc cgcgcgccag cttcgccctc gaggtcattt gcgctcgttg
     2941 tgcgcgaaat cgacgagctt gatcgcctgc tgctccatct cgacgaccag cgcatcgaca
     3001 cgcgagcgga agtaacggta gaacatcatg ctgggaatgg ccacgatgag gcccatcgcc
     3061 gtgttgtaga gcgcgaccga gatgccgtgc gcaagctgga cgggattgga ccccgccgcg
     3121 ctttgcgagc cgaagatctc gatcatgccg atgacggtgc cgaacaggcc gagcagcggc
     3181 gccatcgacg caatcgtgcc gagcgtggtc aggaagcgct cgaggtcgtg cgtcaccacg
     3241 cggccgacct cctcgatcgc ctccttcatg accggacgcg ggcttttcac gttggcgagg
     3301 ccggcggcga ggatgcgtcc gagcgggccc tgttccgccg tgcgcgcaag caggtcgtgc
     3361 gtcggacccg tgtggcggaa ttcgaccagc acgtgatcga ccatgccggc gggtgccacg
     3421 atgctctggc gcagcgacca cagccgctcg aagatcagcg ccagcgccac gaccgacgcg
     3481 atgatgagag gccagatcgg ccagccggcc gcttgaatca tcgcccacat gggtttcgtt
     3541 gctccttcgt tgaagacagc ggcgaaatgt agcgtggcgc ccgcgcggcg gcaaccgcaa
     3601 cgtcaccgcc gtcgcgtcag ccgctcgtcg cgccgcggcg tccgcgccgc ccgcccggct
     3661 cggcaatcgt cgccgagacc gccgcgcccg ccaggatgat cagccagcag aaatagaccc
     3721 acaaaaggaa gatcggcagc gcggcgagcg cgccgtacac cgcctcgtag ctcgaaatgc
     3781 gcgtcaggta ccacgcgaac gcgtgcttgg ccgcttcgaa cacggccgcg ccgatcgcgg
     3841 ctgcgacgag cgcgggcacg agcggcacgt gccgcgcggg caaccatttg tagagcagcg
     3901 tgagtcccgc ggtcgagaac acgaacggca gcgcctcgac gacctgtgcg ccgaatgtcc
     3961 tgcggaacgg aacgaccgcc agcgaatgca cgacgagcca cgtcgtgatc gagatgctcg
     4021 cgccgagcag caccgggccc gcggtgagtc cgatcgcata gacgagcatg cgcttgggga
     4081 gcgaccggcc gcggcggatg ccccagatcg cgttgagctc gcgctcgacg gtggtcacgg
     4141 cgagcacggc gctcaccgcg atgaaggcga tcgagacgcc acgaagccgc ccggcctgct
     4201 cggcgaagcc gatgatgtgg gcgcgcacct gcgcggctcc cgcgaacggc agcaggtggc
     4261 gcagcaggaa gtgctcgagc acgttgagcc agtcctcgaa gatcgggaag cgggcgacga
     4321 acgcgaaggc gacggtgacg agcgggacga tcgcgagcag cgtcgtgaac gacaggctgc
     4381 tcgccgcgcg cgcgagatcg atgtcgcgta gccgcgaagc gacgcggaac aggaagcgga
     4441 acggattcac cgtcgaggcc cgggcgcttt cgccgcaatg agaggcggcc gattatcgca
     4501 gccggtgcgt gatgcagcgc cgtacggcgt aagcttttgc gatattcggg cgaccaacct
     4561 tcgaggagcc tgccatgcca aacgatcccc ctgtcgaaac cgcccttctc ggaggcggct
     4621 gcttctggtg tctcgatgcc gttttccgcg agctcgcggg agtgacgtcg atcgagtcgg
     4681 gatacgccgg cggacacgcg ccgcatccgt cgtacgaggc ggtgtgcagc ggcgacaccg
     4741 gtcacgccga ggtcgtccgc gtgagcttcg accccgcggt gctctcctat cgcgacctgt
     4801 tgcgcgtgtt cttcacgatc catgatccga cgacgcgcaa tcggcagggc aacgacgtgg
     4861 gcacgcagta ccgctcggtg atcttcgcgc tcaccgacgc ccagcgcgcg gatgcgaagg
     4921 cggtgatcgg cgagctcgac gcgcagcacg tctggcccga cccgatcgtc accgacgtgg
     4981 cgggtccggc gacgttctac ccggccgagg cgtatcacca ggactacttc gagcgcaacg
     5041 gcgggcagcc gtactgcgcg tatgtcgtcg cgcccaaggt cgcgaaattc cgcaaggcgt
     5101 acgcggagcg gctcaggaaa cgcgcgtgat gtccgcgttg ccggcacgct agcgacgcgg
     5161 cgcgcgcgtg agccacccgc cggtcgccag gaagacgccg accgaccaca gcaccggcga
     5221 gaggccgacc acgccgccca ccgcgccgaa cacgagcggc acggccaccg agatcgaatt
     5281 gacgagcgac agccgcacgc cggcggcctc gcccatgcgt ccgggcggcg cgtggctgtg
     5341 caacagcgac attaccatcg gctggctggc gccaagtcca agaccgaggc agaacgagag
     5401 ccccatcagc gtgccggccg tgcgcgagaa cggcagcgcg agatagacga gcgcgcctat
     5461 gtaaagcgcc gaggtcagca ccgcatgctc cgatatgcgg cttgcgatcg ccggcatcgc
     5521 gaggcgcacc gtgaacgtcg ccgcggcgaa cgccgcgagg atcaacccga tgcgtgacgc
     5581 ggaaagcccg atcgtgtggc cgtagatcgg cacgaacagc gtgtgcagct cccaggccat
     5641 cgagacgagg ccgttgatga tgaacacgcg ccgcatttcg cgatgcgaga cgaagtcgag
     5701 cgcgctgccg tcgatgcgcg cggcacgcgc gggatgcgcg cctggaagcg cgatccggtt
     5761 ggtcgccagc accgcgatcg gcagcagcgg cagcagtgtc agcaacgcga atgtcgcccg
     5821 gtaccccgca tggtcgatca tgaggcccgc gatcatcggt ccggcgatcg acgacgtcga
     5881 ataaccgagt gcaagcaatc cgaagttgcg cgagcgcgcg gcgaccccgc ccatttctcc
     5941 ggtcgcgtac tgcgcagcca cctggaacag catgaacgac acgccggtca gcatcgccgt
     6001 cgcgaagagc gcgggaagac ccggcagcag cgtcacgacg agcacgcccg cggccgtgcc
     6061 gatcgaaccg gcgagcatcg gcccgcgcac gccgatccga tcggcgagac ggccggcagg
     6121 aatcgcgagg aacatcggga acagcgcgaa gagcgccatc aggccgccga cggtggccgc
     6181 gttctcgccg agtgccagcg cgttcagcga taccgcaacg cgcgatccgg tcagcacgag
     6241 gtgattggcg acgccgagcg cgatcaggac gccgaacggc gatacgcgaa accgctgcgt
     6301 cacggcgcga gccgtccgat cgaccatccg ccgtccgtac gggaccatac gagccgatcg
     6361 tgcagccgtg acggccgccc ctgccagaat tcgaagcggt cggcggcgac gcgcatgccg
     6421 ccccagttcg cgggccgcgg aacatgcgcg gcaaagcgcc cttccacggc ggagaaccgg
     6481 gcctcgagcg cttggcgatc agccaccggg gcgctctgcg gcgacgccca ggcggcaagc
     6541 tgcgagccgt gcgggcgttg cgcgaaatag gcgtccgatt cgttcgcatc cacctgcgcg
     6601 gcgcgcccct cgacgcgcac ctgccgctcg agctcgggcc agaagaagag cagcgcgacg
     6661 cgcgcgttcg ccgcgacgtc gcgtcccttg cggctgtcgt agttcgtatg gaagacgaag
     6721 ccgcgcgcgt cgaccgcctt cagcaggacg atgcgcgcgg ccggttgtcc ggccgggtcg
     6781 acggtggcga gggtcatcgc gttcggctcg ggaaccgcgg cggcgagggc ctcgtcgaac
     6841 cagcgcgcga actgccggta agggtcggct gccgcgtcgc gctcgctcaa tgcggcgcgc
     6901 ttgtagtcga cgcgaagctg tgcaagatcg gttgcgggag tgggcatcgg gcgcgaatgg
     6961 cgatcgattc tactccgccg ccaccgctcg ccgagcggaa cgcaggggga aagcacgcgt
     7021 ggcgcacacg cgttcgcgcc gttgcaacac gctccgctcc agagtggaat ttccctcgta
     7081 cggcgcttcc tccctgcgca aaatgcggta tagtcgccgc cgatggagcg tcgtgacgat
     7141 ggatagtgaa ggcgcgcccg aggacgctgc agcgtaccgc ccgcaaaaac tgacaggaag
     7201 gagaaacatg gctactgcca agaaagctgc gaaaaagacc gcggccaaga agccggccgc
     7261 caggaagagt gctgcaaaga agccggccgc gaagaaga
//
LOCUS       DASSVX010000187         9405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_74160, whole genome shotgun sequence.
ACCESSION   DASSVX010000187 DASSVX010000000
VERSION     DASSVX010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74160"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..852)
                     /locus_tag="VFO53_07915"
     CDS             complement(46..852)
                     /locus_tag="VFO53_07915"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876239.1"
                     /translation="MSAKIEARGLCKTYVSARTGEEVEALRDISFRIMPNEFVSIVGP
                     SGCGKSTLLSLVAGFTAPTTGQVLVDATAVAGPDPRRGVMFQEYALFPWRTVLGNVEF
                     GPMARGARADERRALAQRYIEMVGLAGFEGRYPHELSGGMRQRCALARLLANEPEVWL
                     MDEPLAAVDLQTRNILQDELLRLWGDGGDPAARRSVMFVTHGIDEAVYLSDRVIVLGR
                     RPGQIKEVVAIDLPRPRRAVRNSAEAGRYNDHIWSLIRDEAAQAIVEETS"
     gene            complement(855..1631)
                     /locus_tag="VFO53_07920"
     CDS             complement(855..1631)
                     /locus_tag="VFO53_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876240.1"
                     /translation="MTDAQRRWLSLPVFGTILVFWELAVRQSGQLKLYPYPSGVIEAA
                     VRTIGDGSLIAATSGSLYRVVVGFAIGGVLGIPLGLLMGSLQPVNRAMSPIIDSLRSI
                     APIAWIPMALLWLGIRGNAALFIVAYAAFFPFVVNTIQAVRLIDRNLVNAARALGASR
                     ALVVRSVIMPGSLPVILTGARISLAFAWGAIIAAELAMGIKVTAGGSGAVGLGQLMVS
                     TLYIRRDVNGLVLYMIVVGIISLLIDLLMRRLQRNLTPWR"
     gene            complement(1628..2452)
                     /locus_tag="VFO53_07925"
     CDS             complement(1628..2452)
                     /locus_tag="VFO53_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876241.1"
                     /translation="MSGALRSAAPLPVTMRAGVAWKRHLRHGEFVLALVAVGGLWHLV
                     SVIVNNRTLVPPPLRVLRAWVSLWGEQLPTDIIASLVHLGLGYGLGIATGMVLALLAA
                     RFLFVEVIIDPIVEVLRPISAIAWIPIAILMFGIGTGVPIFLIFYAAIFPVFINMLAG
                     IKQVDPRLVQAAQMLGASRRMIVTHVVMPAALPLILAGARLSLGVAWMAMVAAELTGA
                     DSGLGWRIFWYQEFFAMDKVMAVILTIGVLGYIFDSLLRALQARITRWSPDGSAQQ"
     gene            complement(2449..3606)
                     /locus_tag="VFO53_07930"
     CDS             complement(2449..3606)
                     /locus_tag="VFO53_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET8876242.1"
                     /translation="MTAVDIAALSARVSDQTVWTFVRLETADGRVGWGEATLHNCAAA
                     VHAHVRRLAPAIVGRKSSFPTDVVDVVGISGRDAAEAAAISAIDQALWDVTAQARGEA
                     LANALGDPLRTTIDLYANINRGTLDRSAAGFAARAIEAVASGFDAIKIAPFDGVQPDD
                     VDKTDAINKLGLGLRRIAAVRAAIGPDVRLLVDCHWRLTEILAHDVLREVESLQLYWL
                     ECPLPEDIGHFPALRRLRSRANAMGVRLAGCEMMIGRRGFRPFLDAGVYDVIMPDVKY
                     AGGVRELVRIAQAAAVQGVACSPHNPSGPIAHAHSLHVSAHVPLLPFLEIQYGESPLF
                     FDFVDGALPHPRCGRSDLPRGNGLGVGLARAALRERLIDIEDAATLREVAR"
     gene            complement(3613..4281)
                     /locus_tag="VFO53_07935"
     CDS             complement(3613..4281)
                     /locus_tag="VFO53_07935"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8876243.1"
                     /translation="MEKTASIEICDRIRDDIVSGALPFGSRLTLHRLATRYATGHMPV
                     REALRQLQGEGLVVQAPNCGARVRAVDVDFVRNIFDLRIVIESMLARRAAERIKPKQV
                     AALDVVQAGLETCIAARDFAGALAANRAFHRIINEVAGNPEASALLDRHWGLIAALWN
                     LYGYGEERAAGVISDHHQMLDAFATHDTDVAACLAMAHAAKAKQELIARMLRQPVAVA
                     EAAA"
     gene            4395..5192
                     /locus_tag="VFO53_07940"
     CDS             4395..5192
                     /locus_tag="VFO53_07940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8876244.1"
                     /translation="MSAWFSMSGRRALITGASLSIGRSIALGFADHGAAIAVHYSAAA
                     DKAFGRPDAAQETRMALQSRGVDACVVEADLAEDDAGRRVVAAATAALGGVDILVICA
                     SIQSRAPFLEVTGDEIQRQFRINFRATVELLQAALPAMKANRWGRVLTIGSINQVRPE
                     PDLAIYAALKSAQQNLCVNLAKQYAPFNVMINNLSPGLVATERNRWRRQDAEVWKNIQ
                     ATAARPIGRAADPDEMVGAAVLLCSDAASYIAGTDLMVTGGGHLPLV"
     gene            5291..5812
                     /locus_tag="VFO53_07945"
     CDS             5291..5812
                     /locus_tag="VFO53_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET8876245.1"
                     /translation="MSSSAIRIRNARGELVEPARISSSDAKNGFGRILERVTKEGGVA
                     ITLRNEPKFVVISVETYQRMARADSRSLNTLTDEFDALLERMQEPGVRAAMERAFSMT
                     PEQLGHAARRQGGVVSSGHVAPTVASHVRKGKTLPSGVSGTVVRVRKPATAADVALTK
                     RSAGKKTKHRTRG"
     gene            5805..6494
                     /locus_tag="VFO53_07950"
     CDS             5805..6494
                     /locus_tag="VFO53_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HET8876246.1"
                     /translation="MAKAATPPQRHGSIFVLAGCNGAGKSSIGGAALLASGVRFYDPD
                     EAARKIAAANAHLNPPPTMGEVNAAAWSQGRTLLERAIDQHLDFAFETTLGGQTMTDL
                     LERAAGEGLAVHVWFAGLKNVELHVERVRQRVARGGHDIPREKIVERYDGGRKNLVRL
                     LPQLASVRVYDNSAEADPGRGLTPALRLLLHCVERKIIAPTKLRSLLADTPQWAKPIV
                     AAALDLHLKRR"
     gene            complement(6551..6626)
                     /locus_tag="VFO53_07955"
     tRNA            complement(6551..6626)
                     /locus_tag="VFO53_07955"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6591..6593),aa:Asn,seq:gtt)
     gene            complement(6745..7410)
                     /gene="pgsA"
                     /locus_tag="VFO53_07960"
     CDS             complement(6745..7410)
                     /gene="pgsA"
                     /locus_tag="VFO53_07960"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET8876247.1"
                     /translation="MIPNIPTALTWLRIVLIPVFVGIYYIPQEWLTPVMANWIGMSVF
                     ALAAVTDWLDGYLARRLGATSAFGAFLDPVADKLMVAAALILLVELGRAEAYLAIIII
                     GREIAISALREWMAQLGRSRKTAVAFIGKVKTVAQMTALIALLLWEDVIPGISTPLLG
                     TFALWIAAILTLWSMFHYLRRAAPHFRAEALPRAAEPVPRKPEPRSRKSKPGVRAARP
                     RAH"
     gene            complement(7488..9347)
                     /gene="uvrC"
                     /locus_tag="VFO53_07965"
     CDS             complement(7488..9347)
                     /gene="uvrC"
                     /locus_tag="VFO53_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET8876248.1"
                     /translation="MTKVSRLNRRVVDAEAGATEFDAHELIASLPHLPGVYRMFDAAG
                     GALYVGKARDLKKRVASYFQKGGHEPRIAAMIGQVARVETTVARSEGEALLLENNLIK
                     SLEPRYNILYRDDKSYPYICLSGDPYPQLRFHRGALDRRHRYFGPFPGAGAVREGIAL
                     LQKVFQLRTCENTVFANRSRPCMLHQIERCTAPCVALISEADYQQDVAAAQLFLQGKT
                     DEVLAQLKRQMDAAAAALEFERAARTRDKIARLQQLQSRQFVESATASDIDVVAAVHE
                     QNVFAVNAVMIRGGRHVGDRTFFPRHADPGDDVSDVVSAFLEQHYVDRPVPRTIVAPQ
                     ASDIQALAEVLTEQAVHKVEIVTNPGGERRVWVTMATQNATLAIRQKLAQKATQEDRL
                     AALQEALQLPPSVQRIECFDVSHTMGEAPVASCVIFDKLAMQTSEYRRFNVTPAHAGD
                     DYAAMREALTRRCARIVSGEYPAPDLLVIDGGRGQVNVAADVLAEQGLHQTALIGIAK
                     GPERKPGLEDIVFPGREAVLKLPSDHPGLHLLQQIRDEAHRFAIQGHRARRAKARNTS
                     TLQEISGIGAKRRQALLAHFGGLKGMQSASVEDLARVPGISHALAERIYGELH"
BASE COUNT         1710 a         3147 c         3058 g         1490 t
ORIGIN      
        1 gtcgttcatg tttcctccga agctttctgt tagaacgcga acgtgtcagc tcgtctcctc
       61 gacgatcgcc tgcgcagctt cgtcgcgaat caagctccag atgtgatcgt tgtagcgtcc
      121 ggcctccgcg ctgttgcgca ccgcgcgccg cgggcgcggc agatcgatcg cgacgacctc
      181 cttgatctgg ccgggccgcc ggccgagcac gatcacgcga tccgacagat aaaccgcctc
      241 gtcgatgccg tgcgtcacga acatcacgga gcgccgcgcc gccggatcgc cgccgtcacc
      301 ccacaggcgc agcaattcat cctgaaggat gttgcgcgtc tgcagatcga ccgccgcgag
      361 cggctcatcc atcagccaca cttcgggctc gttggcgagc agccgcgcga gcgcgcagcg
      421 ctggcgcatg ccgccggaca actcgtgcgg atagcgcccc tcgaaaccgg cgagcccgac
      481 catctcgatg tagcgctgcg ccaaggcgcg acgctcgtcg gcgcgtgcgc cacgcgccat
      541 gggtccgaac tcgacgttgc cgagcacggt gcgccaggga aaaagcgcgt actcctggaa
      601 catcacgccg cgtcgcgggt cgggaccggc caccgccgtt gcgtcgacca ggacctgccc
      661 cgtcgtcggt gcagtgaacc cggcgacgag cgacagcagc gtcgattttc cgcagcccga
      721 cgggccgacg atgctgacga actcgttcgg catgatgcga aacgagatgt cgcgaagcgc
      781 ctccacttct tcgccggtac gcgccgacac gtacgtcttg cagaggccgc gcgcctcgat
      841 tttcgcgctc atcgttaccg ccatggcgtg aggttgcgtt gcagtcgccg catcaggagg
      901 tcgatcagca ggctgatgat gccgacgacg atcatgtaga ggacgaggcc gttcacgtcg
      961 cgccggatgt agagcgtgct gaccatgagc tgaccaagcc cgacggcgcc cgatccaccg
     1021 gccgtgacct tgatccccat ggcgagctcg gcggcgatga tcgcgcccca ggcaaaagcc
     1081 agcgagatac gcgccccggt caggatgacc ggcagcgagc ccggcatgat gacggaccgc
     1141 acgacgagag cgcggcttgc accgagcgca cgcgccgcgt tgacgaggtt gcggtcgatc
     1201 agtcgcaccg cctggatcgt gttcacgacg aacggaaaga atgccgcgta cgcgacgatg
     1261 aacagcgccg cgttgccgcg aatgccgagc catagcagcg ccatcggaat ccaggcaatg
     1321 ggcgcaatgg agcgcagcga gtcgatgatc gggctcatcg cacggttcac cggctgcagc
     1381 gagcccatca gcaggccgag cgggatgccc agcacgccgc cgatcgcaaa accgaccacg
     1441 acgcggtaga ggcttcccga tgtcgcggcg atcagacttc cgtcgccgat cgtgcgcacg
     1501 gcggcctcga tcacgccgct cggatacgga tagagcttga gctgtcccga ttgacggacc
     1561 gcgagctccc agaagaccag gatcgtgccg aacaccggca gagacagcca gcggcgctgc
     1621 gcgtcggtca ttgctgcgcc gacccgtccg ggctccagcg ggtgatgcgc gcctgcagag
     1681 cgcgcagcaa cgaatcgaag atgtagccca ggacgccgat ggtcaggatc accgccatca
     1741 ccttgtccat ggcgaagaat tcctgatacc agaagatccg ccatcccagc ccggaatcgg
     1801 cacccgtcaa ctccgcggcg accatcgcca tccacgcaac gccgagactc aaccgtgcgc
     1861 cggcgagaat cagcggcagg gccgccggca tcacgacgtg cgtgacgatc atccggcgcg
     1921 acgcgccgag catctgggcg gcctggacca gccggggatc gacttgcttg atgcctgcca
     1981 gcatgttgat gaacaccgga aagatcgcgg cgtagaagat gagaaagatc ggaacgcccg
     2041 tgccgatgcc gaacatcagg atggcgatgg ggatccaggc gatcgcactg atcggacgca
     2101 gcacctcgac gatcggatcg atgatcacct cgacgaacag aaagcgggct gcaagcaggg
     2161 cgagcaccat gcctgtggcg atcccgagcc cgtagccaag tccgagatga acgaggctcg
     2221 cgatgatgtc ggttggcagc tgctcgcccc acaggcttac ccacgcgcga agcacgcgca
     2281 gcggcggcgg aacgagcgtc ctgttgttca cgatgacgct gacgagatgc cacaggccgc
     2341 cgacagcgac gagggccagc acgaactcgc cgtggcgcaa atggcgcttc cacgcgacgc
     2401 cggcgcgcat tgtcaccggc agcggtgcgg ccgatcgcaa tgcaccgctc atcgcgcaac
     2461 ctcacgtaac gtcgcggcgt cttcgatgtc gatcaggcgt tcgcgcaatg ctgcgcgcgc
     2521 gagccctact cccaggccgt tgccccgcgg cagatcgctg cggccgcatc gcggatgcgg
     2581 caaggcgccg tcgacgaaat cgaaaaacaa cgggctctcg ccgtactgga tttccagaaa
     2641 cgggagcagc ggcacgtgcg cgctgacatg caggctatgc gcgtgtgcga tcggaccact
     2701 cggattgtgc ggcgagcagg caacgccttg aacggcggcc gcctgtgcaa tccgtacgag
     2761 ctcgcgaacg ccgccggcat atttcacgtc cggcatgatg acgtcgtaga cgccggcgtc
     2821 gaggaacggc cggaaacctc ggcggccgat catcatctcg catccggcga gccgtacgcc
     2881 catggcattg gcgcggcttc gcaaacgccg aagcgcggga aaatggccga tgtcttcggg
     2941 cagcggacat tcgagccaat agagctgcag cgattcaacc tcgcgcagca cgtcgtgcgc
     3001 gaggatttcg gtcagccgcc aatggcaatc gacgagcagc cgcacgtccg gtccgatcgc
     3061 cgcgcgcacg gcagcgattc ggcgaaggcc gagccccagc ttgttgatgg cgtccgtctt
     3121 gtcgacgtcg tccggctgca cgccgtcgaa aggagcgatc ttgatcgcat cgaacccgct
     3181 tgcgaccgcc tcgatcgccc gcgcggcaaa cccggcagcg ctgcgatcga gggtcccgcg
     3241 attgatgttc gcataaaggt cgatcgtggt tcgaagcgga tcgcccagcg cgttggccag
     3301 cgcttcgccg cgcgcttgcg ccgtaacgtc ccacagcgcc tggtcgatcg cgctgatcgc
     3361 cgccgcctct gcggcatcgc gtccggagat gcccacgacg tcgacgacgt cggtcgggaa
     3421 cgacgatttt cgtccgacga tcgccggcgc gagccgccgc acatgagcat gaaccgcagc
     3481 ggcgcaattg tggagcgtcg cctcgcccca accgacacgg ccgtccgctg tctcgagacg
     3541 aacgaatgtc cagaccgtct ggtcggaaac gcgcgccgac agcgcggcaa tatcgacggc
     3601 cgtcaccacg actcaggctg ctgcctcggc aacagcgacg ggctggcgaa gcatccgcgc
     3661 gatcaactcc tgcttggctt tcgcggcgtg cgccatggca agacaggcgg ccacgtcggt
     3721 atcgtgcgtg gcaaaggcgt cgagcatctg gtgatggtcg gagatcaccc cggcggcccg
     3781 ctcctcgcca tagccgtaca gattccaaag ggcggcgatc aggccccagt ggcggtccag
     3841 cagcgccgac gcttcgggat tgccggcgac ctcgttgatg attcgatgaa acgcccggtt
     3901 cgcggccaac gcgccggcga aatcccgcgc cgcgatacac gtctcgagac ccgcttgcac
     3961 gacgtcgagc gcggcaacct gcttcggctt gatccgctcg gcggcgcgtc gcgcgagcat
     4021 cgattcaatg acgatccgca aatcgaagat attgcgcacg aaatccacgt cgaccgcgcg
     4081 cacccgggcg ccgcagttgg gcgcctgcac caccagacct tcgccctgga gctggcgcaa
     4141 cgcctcgcgc accggcatat ggccggtggc gtaacgggtc gcgagccgat gcagcgtcag
     4201 ccggctgccg aacggcaagg cacccgacac gatgtcatcg cgaatccgat cgcaaatctc
     4261 gatcgatgcg gtcttctcca tggcaccaaa agtaccacgc ggcggaaatg tgatcaaggt
     4321 cgacgaaaca tcaggatctc tcatggggta gagtgaggcg cgtaaccgtg atttgatcaa
     4381 gcttgggagt caccatgtca gcatggtttt cgatgtccgg gcgtcgtgcc ctgatcacgg
     4441 gtgcgagcct cagtatcggc cgcagcatcg ccctgggatt cgccgaccac ggcgccgcca
     4501 ttgccgtcca ctactcggcc gcggcggaca aggcgttcgg ccggcccgat gccgcgcagg
     4561 agacgcgtat ggcgctacag tcgcgcggcg ttgatgcctg tgtggtcgaa gccgatctcg
     4621 cggaggacga cgccgggcgc cgggtagtcg ccgccgcgac cgctgcgctg ggcggcgtgg
     4681 acatcctcgt catctgcgcc tcgattcaat cgcgcgcacc gttcctcgaa gtgaccggcg
     4741 atgaaataca gcgccagttt cggatcaact tccgcgcaac ggttgaactg ctgcaggcgg
     4801 ccctaccggc gatgaaggcg aatcgctggg gacgcgtgct gaccattggc agcatcaatc
     4861 aggtgcgacc ggagccggat ctcgccatct acgccgcgct caagagcgca caacaaaatt
     4921 tgtgcgtcaa cctggcgaag cagtacgcgc cgttcaacgt catgatcaac aatttgtcgc
     4981 ccggcctcgt tgcgaccgag cgcaaccgct ggcgtcggca ggatgccgag gtgtggaaga
     5041 acattcaggc gaccgctgca cgacccatcg ggcgcgccgc agatcccgac gagatggtcg
     5101 gggccgccgt tctcctctgc tccgatgctg ccagctacat cgcgggaacc gatctgatgg
     5161 tcacgggcgg cggacatcta ccgctggttt gatccaatgg ctgaccaagg aagaagcgcg
     5221 gccccatttg gctaagttgg ctgtgttggc caactcgcgt agaatgactc ttctcgccaa
     5281 aggagtggcc atgtcgtcgt ccgccatcag gatccgcaac gcccgcgggg aactcgtcga
     5341 gcccgccagg atttcgtcga gcgacgccaa gaacggcttc ggccggatac tcgagcgcgt
     5401 gacgaaggag ggcggggtcg cgatcaccct gcgcaacgag cccaagttcg tggtgatctc
     5461 ggtcgagacg taccagcgca tggcgcgcgc ggattcgcgc tcgctcaata cgctgaccga
     5521 cgaattcgac gccttgctcg agcgcatgca ggaaccaggc gtccgggccg cgatggagcg
     5581 cgcgttcagc atgacccccg aacaactggg gcatgcggcg cggcggcagg gtggcgtggt
     5641 gtcgagcggg catgtggcgc cgacggtcgc gagccatgtg cgcaagggca agacgttacc
     5701 ctccggggtg agcgggacgg tagtacgcgt gcgaaagccg gccacagccg cggatgtggc
     5761 gctgaccaag cgtagtgccg gcaagaaaac caagcaccgc acgcgtggct aaggcggcaa
     5821 cgccgccgca gcgtcacggc agcatcttcg tgctcgccgg ctgcaacgga gccggcaaga
     5881 gcagcatcgg gggtgcggcc ctgcttgcgt cgggggtgcg cttctacgac ccagacgagg
     5941 ccgcgcgcaa gatcgcggcc gccaatgcgc acctgaatcc accaccgacg atgggcgagg
     6001 tcaacgcagc cgcttggagc cagggtcgga cgctcctcga gcgcgcgatc gaccagcatc
     6061 tcgacttcgc cttcgagacc accttgggcg gccagaccat gactgacctt ctcgagcgtg
     6121 cggcaggcga gggcctcgcc gtgcacgtct ggtttgccgg tctcaagaat gtcgagctcc
     6181 acgttgaacg cgtgcgccag cgcgtcgcgc gcggcggcca cgacatcccg cgcgagaaga
     6241 ttgtggagcg ctacgacggt gggcgcaaaa atctcgtccg cttgctgcca cagcttgcgt
     6301 cggtgcgggt ctacgacaac tccgcggaag cggacccagg gcgaggtctg acgcccgcac
     6361 tgcggctcct gctacattgt gtcgagcgga agatcatcgc gccgacgaag ctgcgctcgc
     6421 tgctggcgga cacgccccag tgggccaagc cgattgtggc ggccgccctc gaccttcatt
     6481 tgaagcgccg ctgacgaagg ccgcgcacgg cggctaatct cgagccttgg gacagactat
     6541 ttgaatgaat tggctccccg accagggctc gaacctggga cctgcggatt aacagtccgt
     6601 cgctctacca actgagctat cggggatcgg gagaagcgcc gcagccgctt tgaccgctac
     6661 gtcgcggcgc gaattttaac atgccggcca cgcgcgcatc gcggcgcgcc acaccgcgat
     6721 attcagccag cgcgcgcctg ccctctaatg cgctcgcggc cgcgcggcac gcacgcccgg
     6781 cttggacttg cgcgagcgcg gttctggttt ccgcgggacc ggctccgctg cgcgcggcaa
     6841 cgcctcggcg cggaagtgcg gcgcggcgcg tcgcaggtag tggaacatcg accacagtgt
     6901 caggatcgcc gcgatccaca atgcgaacgt gccgagtagt ggtgtggaaa tcccgggaat
     6961 cacgtcttcc cacagcagta gcgcaatcaa tgcggtcatc tgcgcaaccg tcttgacctt
     7021 gccgatgaag gcaaccgcgg tcttgcgcga gcggccaagc tgcgccatcc actcgcgcaa
     7081 tgcggaaatc gcgatctcgc ggccgatgat gatgatggca aggtacgcct cggcacgtcc
     7141 gagctcgacg agcaggatca acgcggcggc aaccatcagc ttgtccgcaa cgggatcgag
     7201 gaacgcgccg aacgcactcg tcgcgccgag gcggcgggcg aggtagccgt cgagccagtc
     7261 ggtgaccgcg gcgagcgcga atacgctcat gccgatccag ttggccatca ccggcgtgag
     7321 ccattcctgc ggaatgtaat agatgccgac gaacaccggg atcagcacga tccgcagcca
     7381 cgtcagggcg gtgggaatgt tcggaatcat ttgcgctgcg gcggacgaca acccgatgat
     7441 tctacgctgc gaaccggatc cggccgccgc gcgacgatcg acggtcttta gtgcaactcg
     7501 ccgtaaatac gctcggccag cgcatgcgat ataccgggta cccgcgcgag atcctcgacg
     7561 ctcgccgact gcattccttt cagcccgccg aaatgcgcaa gcaacgcctg gcggcgcttg
     7621 gcgccgatgc cgctgatctc ctgcagtgtc gacgtgttgc gcgccttcgc gcgccgcgcg
     7681 cgatgcccct ggatcgcgaa gcgatgcgcc tcgtcgcgaa tctgctgcag caggtgcagg
     7741 cccgggtgat cggacggcag tttgagcact gcttcgcggc cggggaacac gatgtcctcg
     7801 aggcccggct tccgctcggg gcccttcgcg atcccgatca gcgccgtctg atgcaacccc
     7861 tgctcggcca gcacgtcggc ggcgacattc acctgcccgc ggccgccgtc gatcacgagc
     7921 agatccggcg ccggatattc ccccgacacg atgcgcgcgc agcgccgcgt cagcgcctcg
     7981 cgcatcgccg catagtcgtc gccggcgtgc gccggagtca cgttgaagcg gcggtactcc
     8041 gacgtctgca tcgctagctt gtcgaagatc acgcacgatg cgaccggagc ctcgcccatc
     8101 gtatgcgaca cgtcgaagca ctcgatgcgc tgcacgctcg gtggaagctg caacgcctcc
     8161 tgcagcgccg cgaggcgatc ctcctgcgtc gccttctgcg cgagcttctg gcggatcgca
     8221 agcgtcgcgt tctgcgtcgc catcgtcacc catacgcgcc gctcgccgcc cgggttcgtc
     8281 acgatctcga ccttgtgcac cgcctgctcg gtcagcacct cggcgagcgc ctgaatgtcg
     8341 ctggcttgcg gcgcgacgat ggtccgcggc accgggcggt cgacgtagtg ttgttccagg
     8401 aacgcgctga ccacgtcgct tacgtcgtcg cccggatccg catgccgtgg aaagaacgtg
     8461 cggtcgccaa cgtgacggcc gccacggatc atcaccgcat tcacggcaaa cacgttctgc
     8521 tcatgcacgg cggcaacgac gtcgatgtcg cttgccgtcg cgctctcgac gaactgccgc
     8581 gactgcagtt gctgcaggcg ggcgatcttg tcgcgcgtgc gcgccgcgcg ctcgaactca
     8641 agcgccgccg cggccgcatc catctgccgc ttgagctgcg cgagcacctc gtcggtcttg
     8701 ccctgcagga agagctgtgc ggcggcgacg tcctgctggt aatcggcctc gctgatcagc
     8761 gccacgcacg gcgcggtgca gcgctcgatc tggtgcagca tgcagggccg cgaacgattg
     8821 gcgaacacgg tgttttcaca ggtgcgcagc tggaacactt tctgcaatag cgcaatgccc
     8881 tcgcgcaccg cacctgcgcc gggaaacggt ccgaagtagc ggtgccggcg atcgagcgcg
     8941 ccgcggtgga agcgaagttg cggatacgga tcacccgaaa ggcagatgta cggatagctc
     9001 ttgtcgtccc gatacaggat gttgtagcgc ggctcgagcg atttgatcag gttgttctcg
     9061 agcagcagcg cctcgccctc cgagcgggca accgtcgtct cgacgcgcgc cacctggccg
     9121 atcatcgcgg caatccgcgg ctcgtggccg cccttctgga aatagcttgc gacgcgtttc
     9181 ttgagatcgc gcgccttccc gacatagagc gccccacctg ccgcgtcgaa catccggtag
     9241 acgccaggca ggtgcgggag cgatgcgatg agctcgtgcg cgtcgaattc ggtggctccc
     9301 gcttcggcat cgacgacgcg gcggttgagg cgcgatacct tggtcatcgc gcgggccgtc
     9361 cgaggtgatc ggcgatcgcg tcgaacaccg aatgaaacat cgccg
//
LOCUS       DASSVX010000188        14040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_807710, whole genome shotgun sequence.
ACCESSION   DASSVX010000188 DASSVX010000000
VERSION     DASSVX010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 14040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14040
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807710"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..462)
                     /locus_tag="VFO53_07970"
     CDS             complement(91..462)
                     /locus_tag="VFO53_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876249.1"
                     /translation="MTGARPGTPGATRACPHCRETILESSGVCPACRHYLRFDAGGDA
                     APAPAITPLRIEGGIRHPADADPWEYTVVVTIRNGRGEEIARKLIGVGALDASDQCTF
                     ALSVEAVTAKGKRPGKGGTRH"
     gene            complement(505..930)
                     /locus_tag="VFO53_07975"
                     /pseudo
     CDS             complement(505..930)
                     /locus_tag="VFO53_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006449865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
     gene            complement(1195..2019)
                     /locus_tag="VFO53_07980"
     CDS             complement(1195..2019)
                     /locus_tag="VFO53_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HET8876250.1"
                     /translation="MPMYMDIHEVHGATAEDVARAHVADVQTQEKYGVVYHKYWFNES
                     RGKIFCLCSAPSAEAATRVHREAHGLVASKIIEVEPEIADGFLGGSEVNPAGAAVVPG
                     GTANDRDPGIRTIMFTDIVDSTALTQRLGDDAAMAFLDVHDTIVRDALAALGGREVKH
                     TGDGIMACFLSTVSAVRCATRIQRALAQHAQTVTEVQVRVRIGAAAGEPVENHTDLFG
                     STVQLAARLCSHAQPQQSLVSNAVAELCLGKGLSFEDLGEVSLKGFERPVRVHAVQ"
     gene            complement(2138..2575)
                     /locus_tag="VFO53_07985"
     CDS             complement(2138..2575)
                     /locus_tag="VFO53_07985"
                     /inference="COORDINATES: protein motif:HMM:NF015709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HET8876251.1"
                     /translation="MTDEIAVFDIDGTLADVSERLHHIRTKPKNWDAFFAAMAQDKAI
                     ASVVRLCNLLHAAGVHVVLCSGRPERYRAETVTWLAREGVRYHELRLRRDGDRRSDTI
                     AKREMLAGIERTRVLFIVEDRSSVVEMWRAQGFVCLQCAPGEF"
     gene            complement(3080..3394)
                     /locus_tag="VFO53_07990"
     CDS             complement(3080..3394)
                     /locus_tag="VFO53_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876252.1"
                     /translation="MLRMLRPAPVVFVERRLDETFDVIGFGSGDLSDLLQTLLGVAAD
                     LLDLIAHLLGEADRLLLGEAAHLPLHFDDSLARLSRRIEHLFGAGREEASRAIVANVE
                     QR"
     gene            complement(3640..5088)
                     /locus_tag="VFO53_07995"
     CDS             complement(3640..5088)
                     /locus_tag="VFO53_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8876253.1"
                     /translation="MAPSGGRITDLGWICALYELGQKAANGAHPQQVRQEILEHIVRG
                     FDAESGSIALIVDGTVDQLELAAGTDLPIGLIGKPLPRGTGVFGHVVATGQPVLVNGD
                     AAQTGLPLRASAERQRSTHSAMCWPLRVAEHTIGAVAVNRAETSPRYTVDDLDQGQAL
                     TSLLALVMANHRMHVERDNRILELSTLNATMQRINGMLEDAQNQVIQSEKLASIGQMA
                     AGVAHEINNPLAFALSNLGSLESYLSRLFDLLGAYIDADAGLGEDNEALAAARRLRSE
                     IDFAFLRRDTADLVAESRDGLLRVKRIVLDLRDFSRGGGEEVWQAIDVHAALDRTLNI
                     VRNELKYKAHIVRDYGALPEIECMPSRLNQVFLNLLVNAGQSIGANGTITLSTGTERD
                     EAWIAVADTGCGIAPENLNRIFDPFFTTKPVGEGTGLGLSVSHSIVRKHGGRIDVESE
                     IGRGTRFTVRLPLAQRATSGAQAPTGVETSAA"
     gene            complement(5139..5681)
                     /locus_tag="VFO53_08000"
     CDS             complement(5139..5681)
                     /locus_tag="VFO53_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8876254.1"
                     /translation="MSSLLIVDDEPNVLSAIRRLCQNGAIAPAIPDLSIATFTSPHDA
                     LRYLGDHAVDIVISDYRMPQMDGAAFLALVRERHPDAARIIMSACSDIDGIVRAVNHA
                     GIFRFVSKPWSDPDLKATIIEVLAHRGLLLENRVLADEVRAQRNVILRQKVELERLER
                     ESPGITKVRWTEDGGVLLEE"
     gene            5945..7648
                     /locus_tag="VFO53_08005"
     CDS             5945..7648
                     /locus_tag="VFO53_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876255.1"
                     /translation="MPTARMRRIMPSIHPHLCLVAAALCAALAAAPAMAESGRQTVCT
                     ITVNSSDEKRAFERYLPPGKFEFVELVERGRPDWLESARRKGIRCDILVISGHYDGGD
                     YAGGNEFFSEHVDSREYLPVDEMERVACSNPGDGLFSHLKAVYLFGCNTLNPVALRSA
                     PEEIARTLVQSGHAPAEAERLARAMSARFADSSRDRMRHIFRNVPAIYGFSSVAPLGP
                     LAASYLDRYFQAGGANELAAGRPGAKLLAYFPGHSLTVTRGSTDADPDAGFRRDTCQF
                     LDDRLSPLRRAQFVHALLGRDMAEVRLFLDRLERYAASLAHVRAAAPESIDELQAIAT
                     DRPARERYLAFMRATDEAPLRLRMIDLARDLGWLSPDARIAELEQMIAARYARPLLTA
                     ADVDFVCRLNANRALDGARATLPGATDAGHAAIVACLGDKDQRERLLQALTSPQDADV
                     QIAQSYLQYRPISDVSELRNVADAIARMSDVSAQVRALDTLADHRVTDPQALGELARL
                     FPVARTIDVQRAIAGVLIRSDYEAMDVPELARTLREHRFKSRDGRDLIDVLIRRLEAA
                     A"
     gene            7755..9422
                     /locus_tag="VFO53_08010"
     CDS             7755..9422
                     /locus_tag="VFO53_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876256.1"
                     /translation="MLLPRRIFLAAALLAAFSLQDALAARQTVCTITVNSADEREAFR
                     RYLPADQYDFVELVERGRPDWLASACRQHVRCDVLLISGHFDGGDEFYSDRVDARESL
                     PVDEMERVSCSDSCPGLFSQLKEVYLFGCNTLNAEKVSSTSADVTRALLRSGHSAADA
                     ERVAQLLDARHADSNRDKMRMIFRDVPVIYGFSSKAPLGAKAGPLLERYFRSGTASIG
                     SGRPSEKLLGLFAASSMTVTSGSTDADPQASYRRDMCQFADDRLSAAQRVAFVHSLLD
                     RDVAEVRTFLDSLEKYAASLTPADRADAAVSKGLEAIATDAHAKERWLAFARDADEPA
                     MRARMIALAARLGWLSESSTQAEIARLVRDLYARNELGNTDVELVCTLNRDHALDSAL
                     AGISATPRDRDIAHAALLACAGSASARAELIDALTSGRDRDVGLAQVYLKHHPIADVG
                     DLRAVALRIARMSDVDAQIRALDTLAEHRVSDRESLESLTRLFPVAKTVNVQRAIAGI
                     LIRSDYEAIAKPELVRALREKRLKSPDGQDLIDVLIRRMQASLSRAA"
     gene            9482..10156
                     /locus_tag="VFO53_08015"
     CDS             9482..10156
                     /locus_tag="VFO53_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET8876257.1"
                     /translation="MKLYYHPASTTCRPIMLFAAESGVRLDFQLVDIFTGEQYKEPFT
                     AVNPNHMVPVLEDGDFRLTESSAILKYLADSIDSPAYPKPLRARARVNERMDWINTQV
                     SRDFTYGFVYPQLLPVFKRADAAVQAATLAWGRERARGWLKVLDQHVLGPESRYLCGD
                     AITIADYFAAPFITLGEAIRCDYSAYPNVCRWLSAMKALPSWRKVNEAIDGFAASLKD
                     AQFETL"
     gene            10167..10604
                     /locus_tag="VFO53_08020"
     CDS             10167..10604
                     /locus_tag="VFO53_08020"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET8876258.1"
                     /translation="MARAEPVLAHHDVLAALDIVRREVEAKDRGAAIAIADAHGELIA
                     FVRCDGALIASGTLAINKAFTAARLNRPTRGLGDTLRAKGTDIAFYGDPRYVGFPGGL
                     PVRIGGIVAGAVAVSGLSDDEDETLAGLAIAYLEGRAPVSISA"
     gene            complement(10634..11476)
                     /locus_tag="VFO53_08025"
     CDS             complement(10634..11476)
                     /locus_tag="VFO53_08025"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET8876259.1"
                     /translation="MQSPHAFGIAGLLGWPVAHSRSPVIHNHWIAEHRLPGRYVLFGV
                     APDDLRAAVKGLSALRLRGCNVTTPHKQAVMPLLDDVDPLAQRIGAVNTIVVQEDGSL
                     RGFNNDGNGFVQSIRDARPGWRPDVGPIAVVGAGGAARAVIASLAAQGAREIRVVNRT
                     WERAKSLQDSFGPPLVAVRWDDRDATLGDIALLVNATNQGMIGQPPLDLDLAPLSSTA
                     IVGDLIYVPPETPLLRAARLRGNATVNGLGMLLNQARPAFHAWFGVLPEITPALIRAV
                     EATF"
     gene            complement(11499..12641)
                     /locus_tag="VFO53_08030"
     CDS             complement(11499..12641)
                     /locus_tag="VFO53_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876260.1"
                     /translation="MKAISWLLALALGMTAPLVSAQQGPVPIFGLVELSGTGTTSGTM
                     FDNGVKLAVKEINASGGILGRKIEYTSLDTQSQPQVAKALAQKAIDQGAYVVMGPVFS
                     GSILVSMAETRRAEVPNFTGGEAAAITQQGNPYIFRTSFTQTTAMPKVARYLKDTLKA
                     KNVAIIWVNNDFGKGGLDTMKKALDAQGIKVAAEISTDPGQLDFSGAVLKAKQANADA
                     LFVYTNEEEAARALRELRKQGYDKPIVGETVLTSQKVIELAGDAANGATAHVGLTADA
                     PQPAIHAFDQKFQKEYHTKSDHNGLKGYSAMYVVKAVTEKIGKFDQKAFAKAMHGLHL
                     SAKEHPGVLLDVSFDQNGDLDRESFMTKVENGKQVVTATLPAASAK"
     gene            complement(12684..13913)
                     /gene="pobA"
                     /locus_tag="VFO53_08035"
     CDS             complement(12684..13913)
                     /gene="pobA"
                     /locus_tag="VFO53_08035"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HET8876261.1"
                     /translation="MAPLRTQVAIIGAGPSGLLLGQLLHNAGIDNVVLERRSAAYVLG
                     RVRAGVLEQTSVDLLEAAGVGARMRNEGLPHDGIELSFGGARHRIDFRSLTGKQVTIY
                     GQTEVTHDLMDARRAFGGTTIYAADRVTPHDFDAASPGVTWRSDGTEHRLACDFIAGC
                     DGYHGVSRASVPESSLATYERVYPFGWLGILADTPPVSHELIYSNHARGFALCSMRSP
                     TRTRYYIQCALDDSVDAWSDDAFWDELRRRLDREAAETMVTGPSVEKSIAPLRSFVAE
                     PMRFGRLFLAGDAAHIVPPTGAKGLNLAIADVRFLSRALIEALTERSSAGIDHYSDIC
                     LRRIWKAERFSWWFTSLTHRFPDTGAIGRKLQQAELEYLVASKAASTAMAENYVGLPF
                     EDVPRDPGSLAHSAPRD"
BASE COUNT         2208 a         4748 c         4721 g         2363 t
ORIGIN      
        1 cgagcgcgac gatcaccagc gtatggaagc gcgaccagcg cagcgcgtcg aggcgcgccg
       61 ggaccgcgct ttcgacgatg cgctgatcgg tcagtgccgc gtaccgccct tgccgggccg
      121 ctttcccttg gctgtcaccg cttcgaccga caacgcgaac gtgcactgat cgctggcgtc
      181 gagcgcgccg acgccgatca gcttgcgcgc gatttcttca ccgcgcccgt tgcggatcgt
      241 cacgacgacc gtgtattccc agggatcggc atccgccggg tggcgaatgc cgccttcgat
      301 acgcagcggc gtgatcgcgg gcgccggcgc ggcgtcgccc ccggcgtcga aacgaaggta
      361 gtgccggcag gccggacata cgcccgagct ctcgaggatc gtctcccggc agtgcggaca
      421 ggcgcgcgtc gcgccgggcg tgcccgggcg tgcgcccgtc atcgaggcgg ccgagccgcg
      481 ccaacggcga gcgcgcggcc gcgatcaccc atgcttgccg accttcgcac cgcccttgcc
      541 gtcgtcccag ccgaactcgg cctggccgcg cagccgcgcg ccggcggcga ccgtaagcga
      601 gcccgccttg acgtcgccga tgatcacgcc gctgtgctgc agatcgacca gcgtggcctg
      661 ctcgacgttg ccttccagtt cgccggcgat cgtcaccttg tcggcgcgca cgccaccggt
      721 gagcttcgcg ccggcgtcga tggtgaggtc gccctgcacg ttgacatcgc ccttgaactt
      781 gccggcgatt cgcacgctgc cgccgccctc gatcttgcct tcgatcgtga tgtcggcggc
      841 gatcagcgat tccttgcgcg gtgcggcgtg ctccgcgcgc gccatcggtg ccgcaggagc
      901 cgccggcgcc gggttggcgg ccggctcggg tcgcggcggc ggctcgtagc ggctgggttg
      961 ttccggggcg ggcgtggggt tcacccgggc ggcggtgttg tctttccaga tggccatcgt
     1021 agggacctat cgcgggtcgt tgggggattg caggctagcg ctaaatgggg gcgtaaggca
     1081 accggttttt ggctttttga tgctatcgtc atggccgtcg cggtgcggtt ttgccgaatg
     1141 gcaacacggg ccggccgagg tcccgccccg cccgcggcgt tcgagagcct tcgtctactg
     1201 cacggcgtgc accctgaccg ggcgctcgaa gcccttcagt gaaacttctc ccaggtcctc
     1261 gaagctgagc cccttgccga ggcaaagctc ggccacggcg ttcgacacga ggctttgctg
     1321 cggctgcgcg tgcgagcaga gacgggccgc gagctgcacc gtcgacccga agaggtcggt
     1381 atgattttcg accggttcgc ccgctgcggc gccgattcgt acacggacct gtacttccgt
     1441 caccgtttgc gcgtgctgcg ccagcgcgcg ctggatgcgc gtcgcgcagc gcaccgcgga
     1501 cacggtcgac aggaagcacg ccatgatccc gtcaccggtg tgcttgacct cgcggccccc
     1561 gagagcggcg agcgcatcgc gcacgatcgt gtcgtgaacg tcgagaaacg ccatcgcggc
     1621 gtcgtcgccg aggcgttgcg tcaacgccgt cgaatcgacg atgtcggtga acatgatcgt
     1681 ccggatgccg ggatcgcgat cgttggccgt accgcccggg acgacggccg cgcccgccgg
     1741 attcacttcg ctgccgccga ggaaaccgtc ggcgatctcg ggctcgacct cgatgatctt
     1801 cgatgccacg agcccgtgcg cctcgcggtg cacgcgcgtg gcggcctcgg cgctgggcgc
     1861 gctgcacagg cagaagatct tgccgcggct ctcgttgaac cagtacttgt ggtatacgac
     1921 gccgtacttt tcctgcgtct gcacgtcggc gacgtgcgcc cgggcgacgt cttccgcggt
     1981 ggctccgtga acctcgtgga tgtccatgta catcggcatg gcgacctcgc tcggcgctgg
     2041 atgaacggat gccgcgcaac gctacacgtt caccgtgccg agcgcaacgc gaatcgccgg
     2101 ggccatggaa aacgcggcgc gagcgttcat ccgatgttca aaattcgccc ggcgcgcatt
     2161 gcaggcagac gaatccctgc gcgcgccaca tctccacgac gctgctgcgg tcctcgacga
     2221 tgaagagcac gcgcgtgcgt tcgatgcccg ccagcatttc gcgcttggcg atcgtatcgc
     2281 tgcgccggtc gccgtcacgg cgcagccgaa gttcgtggta gcgcacgcct tcgcgcgcga
     2341 gccacgtcac cgtttcggcc cgatagcgct cgggacggcc ggaacacagc acgacgtgca
     2401 cgcctgcggc gtgaagcaga ttgcaaagcc gcacgacgga agcgatcgcc ttgtcctgcg
     2461 ccattgcggc gaaaaacgca tcccagttct tcggcttcgt gcggatgtga tgaagccgct
     2521 cggaaacgtc ggcgagcgtt ccgtcgatgt cgaagaccgc gatttcatcg gtcatgcgcg
     2581 tgcttgcgca gcgatggtgc attgcggaac ttcgcgacga cgactcgggt cgcatctgtc
     2641 gcgcaggcgc cgaccagcat cgttcaaccg ccgcgtgcgc attctcgata tcacggtcgg
     2701 cgaacggtcg ccggcgcaac gcacgcgctg gtgcgaaagg cacgtcacgc cgcggcatcg
     2761 gacggcaatg caacacgtca cgaaagagga atgggcaatg gatcgcaacc agcaacggca
     2821 aggcggtggg caaccgaatt atggcgccga tcgatcgcaa tcgcgcgctt ccctgcaaca
     2881 aggcggacaa tcgcacggcg gcgcacagca gtcgcagcaa ggcgctttgc ggcaggcgtt
     2941 cggcgggcaa ccattccatg gcatggcgga ggtcgcgttg cgcggaaccg cgctgctctg
     3001 ggatctgcag atggaaaccg cgcgcaacat gtggcgaacg caggcgcgca cggccgcgat
     3061 gctcggcata cccgactgtt cagcgctgtt cgacgttggc gacgatcgcg cgcgacgcct
     3121 cttcacggcc agcgccgaac agatgctcaa ttcggcgcga caggcgcgcg agactgtcgt
     3181 cgaaatgcag cggcagatgg gccgcctcgc cgagcagcag acgatcggcc tcgccgagga
     3241 ggtgcgcgat cagatcgagc agatcggccg ccacaccgag cagggtctgc agcagatcag
     3301 acagatcgcc gcttccgaag ccgatcacgt cgaaggtttc gtccagtcga cgctcgacga
     3361 aaacgacggg cgcaggtcgc agcatgcgga gcaacgcgac gaatcggcgc ccggggacaa
     3421 cgcatccgcg gccaacatgg ccgacgcgca ggcacgaggc ggcgcggggg caaccagcaa
     3481 gaacggcgtg cagaatccga catgggattc cgacaacgcc gagactgatg cgtcaagcga
     3541 agggcgtccg aatcgcgaaa cgcgccgcaa cgaagaacgt tcccgcgcgc gtcgttgagc
     3601 agcgcgcggc acgaacgggg ctcgcggtgg ccgcgagccc tacgctgcac tggtttcgac
     3661 gccagtcggt gcctgtgcgc cggaggttgc gcgctgcgcg agcggcaggc gcacggtgaa
     3721 gcgcgtgccg cggccgatct cgctttccac gtcgatgcgc ccgccgtgct tgcgcacgat
     3781 cgaatgcgaa acggacaacc cgaggcccgt tccttcgccg accggcttcg tcgtgaagaa
     3841 cgggtcgaaa atgcggttca ggttctcggg tgcaatgccg cagcccgtgt ccgcgacggc
     3901 gatccacgcc tcgtcgcgtt cggtgccggt cgacagagtg atcgtcccgt tcgcgccgat
     3961 cgattggccg gcgttgacga gcaggttcag gaatacctgg ttgaggcgcg acggcatgca
     4021 ttcgatttcg ggcagcgcgc cgtagtcgcg cacgatgtgc gccttgtact tgagctcgtt
     4081 gcgcacgatg ttgagcgtcc ggtcgagcgc cgcatgaacg tcgatcgcct gccacacttc
     4141 ctcgccgccg ccgcgcgaga aatcgcgcag gtcgagaacg atgcgcttga cgcgaaggag
     4201 cccatcgcgc gattcggcga cgaggtcggc ggtgtcgcga cgcaggaatg cgaagtcgat
     4261 ttcgctgcgg agccggcgcg ccgccgcgag cgcttcgttg tcctcgccga gccccgcatc
     4321 ggcgtcgatg tacgcgccga ggaggtcgaa cagccgtgac aggtacgatt cgagcgatcc
     4381 gaggttcgac aacgcgaacg ccagcggatt gttgatctcg tgcgcgacgc cggccgccat
     4441 ctggccgatc gacgcgagct tttccgactg gatcacctgg ttttgcgcat cctcgagcat
     4501 gccgttgatg cgctgcatcg tcgcattgag cgtggagagc tcgagaatgc ggttgtcgcg
     4561 ctcgacgtgc atccggtggt tcgccatcac gagcgcaagc aggctcgtca gcgcctgtcc
     4621 ctggtcgagg tcgtccaccg tatagcgcgg gctcgtctcg gcgcgattga ccgccacggc
     4681 gccgatcgtg tgttccgcga cgcgcagcgg ccagcacatc gccgagtgag tcgagcgctg
     4741 ccgctcggcg gaggcgcgga gtggcaggcc ggtctgcgcg gcatcgccgt tcacgagcac
     4801 cggctgtccg gtggccacga catgcccgaa cacgccggtt ccccgaggca acggcttgcc
     4861 gatgaggccg atcggcaggt cggttcccgc ggcaagctcg agctgatcga cggtgccatc
     4921 gacgatcagc gcgatcgacc cgctctccgc gtcgaagccg cgcacgatgt gctcgaggat
     4981 ctcctggcgc acctgctgcg ggtgcgcgcc gttcgccgct ttctgaccca gttcgtacag
     5041 cgcgcagatc cagccgagat cggtgatccg gccgccggaa ggtgccatct cgttgtccag
     5101 ggtcaacggg cgctgcgcat catcggccgc ggcgtttgtc actcttcgag cagcacgccg
     5161 ccgtcttcgg tccagcgcac cttggtgatg ccgggcgact cgcgctcgag ccgttcgagc
     5221 tcgaccttct ggcgcaggat cacgttgcgc tgcgcgcgca cctcgtcggc gagcacgcga
     5281 ttctcgagca ggagtccgcg gtgggcgaga acctcgatga tcgttgcctt gaggtccggg
     5341 tcggaccacg gcttgctcac gaacctgaag atgcccgcat ggttcactgc acgcacgatg
     5401 ccgtcgatgt ccgagcacgc gctcatgatg atgcgcgccg cgtcgggatg ccgctccctc
     5461 accagcgcaa gaaacgccgc cccgtccatc tgcggcatcc ggtaatcgga gatcacgatg
     5521 tcgacggcat ggtcgccgag atagcgcagc gcgtcgtgcg gcgaggtgaa cgtggcgatc
     5581 gacagatcgg ggatcgccgg cgcaatcgcc ccgttctggc ataagcgccg gatcgcgctc
     5641 agcacgttcg gttcgtcgtc gacgatcagc agggaagaca tcgatgttcc ttcggttgct
     5701 tgtcggtgcg cgcgactatg caagggccat gccatgcgat ccgccatcgc tcgtgaagcg
     5761 gtcgactaca acttcggcag cacgatgcgt ggacgtcagc gcatctctcg ccaagtacat
     5821 gctgtcagcg cattcttacg ccggttgcgg atgcgaccga tagcacctcg gtccctggtc
     5881 cgatgtacat gcgggcgcgc agtcgttgaa attgcgctgt cgcccgcggt gcacctcagc
     5941 gggcatgcca accgcccgca tgcgccgcat catgccgtcg attcatccgc acctgtgtct
     6001 cgtcgcggcc gcgttgtgcg cggcactcgc ggcggcaccg gcaatggccg aaagcggcag
     6061 gcagaccgtg tgcacgatca cggtcaattc gtcggatgaa aagcgcgcgt tcgagcgcta
     6121 tctgccgccc gggaaattcg agttcgtcga gctcgtcgag cgcggccgcc ccgactggct
     6181 cgaatccgcg cggcgcaagg gcatacgctg cgacatactc gtcatctccg gccattacga
     6241 cggcggtgat tacgcgggcg gcaacgaatt cttctcggag cacgtcgatt cgcgcgagta
     6301 cctgccggtc gacgaaatgg agcgcgtggc ctgcagcaac ccgggcgacg ggctgttctc
     6361 gcatctcaag gccgtctacc tgttcggctg taacacgctg aatcccgtgg cgctgcgaag
     6421 cgcgcccgag gaaattgcac gcacgctcgt gcagtcgggc cacgcgccgg ccgaggccga
     6481 gcggctggca cgcgcgatga gcgcgcgctt cgcggacagc agccgcgatc gcatgcgcca
     6541 catcttccgc aatgtgccgg ccatctacgg cttttcgtcg gtcgcgccgc tcggtccgct
     6601 cgccgcgagc tacctcgatc gctacttcca ggcaggcggt gcaaacgaac tcgcggcggg
     6661 ccggcccggc gcgaagctgc tcgcgtactt tcccggccac tcgctcaccg tgacccgcgg
     6721 gtcgaccgac gccgatcccg atgccggatt ccgccgcgac acctgccagt tcctcgacga
     6781 ccggctttcg ccgctgcgtc gagcgcagtt cgtgcacgcg ctgctcggcc gcgacatggc
     6841 cgaggtgcgg ctcttcctcg accggctcga gcggtatgcg gcgtcgcttg cgcacgtccg
     6901 cgcagcggcg cccgagtcga tcgacgagtt gcaggcgatc gccacggatc ggccggcgcg
     6961 cgagcgctac ctcgcgttca tgcgcgcaac cgacgaggcg ccgctgcggc tgcggatgat
     7021 cgatctcgcc cgcgacctcg gctggctgtc gcccgacgca cggatcgccg agctcgagca
     7081 gatgatcgcc gcccgctatg cgcgtcctct gctcacggcg gccgacgtcg acttcgtctg
     7141 ccggctgaat gccaatcgtg cgctcgacgg cgcgcgcgcc acgctgccgg gcgcaaccga
     7201 tgccggccac gccgccatcg tcgcctgtct tggcgacaag gatcagcgcg agcggctgct
     7261 gcaggcactg acgagcccgc aggatgccga cgtgcaaatc gcgcagtcct atctgcagta
     7321 tcgcccgatt tccgacgtga gcgagttgcg caacgtggcg gacgcgatcg cgcggatgag
     7381 cgatgtcagc gcgcaggtac gcgcgctcga cacgctcgcg gatcaccggg tgaccgatcc
     7441 gcaggcgctc ggcgagcttg cgcgcctgtt cccggtcgcg cggacgatcg acgtccagcg
     7501 cgccattgcc ggcgtgctga tccgctcgga ttacgaggcg atggacgtcc cggaactcgc
     7561 ccggacgctg cgcgagcacc ggttcaagtc gcgcgacggt cgcgacctga tcgacgtgct
     7621 gatccgccgc ctcgaggccg ccgcctgacg gccatacgcg gttcacgacg cttccggccc
     7681 ttccgatcgg cgggccaccc gtgcaatact tcggcgctgc gcacggcagc gattgcctcg
     7741 ggtattgcac gttcatgctc cttccccgcc ggattttcct cgcggccgcg ctgctcgcgg
     7801 cgttctcgct gcaggacgcg ctcgccgccc gccagacggt gtgcaccatc acggtcaact
     7861 cggccgacga gcgggaggcg tttcgccgct atctgccggc ggaccagtac gacttcgtcg
     7921 agctcgtcga acgtgggcgc cccgactggc tggcctccgc gtgccgccag cacgttcgct
     7981 gtgacgtgct gctgatctcc ggacatttcg acggcggcga cgagttctat tccgatcgcg
     8041 tggacgcacg cgaatcgctg cccgtcgacg agatggagcg cgtctcgtgc agcgactcgt
     8101 gcccgggtct cttttcgcag ctgaaggagg tgtacctgtt cggctgcaac acgctcaatg
     8161 cggagaaggt ttcgagcacg tcggcggacg tgacccgcgc attgctgcgc tcgggtcatt
     8221 cagcggccga cgccgagcgg gtcgcgcagc tcctcgacgc acgccacgcg gacagcaacc
     8281 gcgacaagat gcgcatgatc ttcagggacg tgcccgtcat ctacggcttc tcgtcgaaag
     8341 cgccgctcgg cgcgaaggcg ggtccgctgc tcgagcgcta tttcaggtcg ggcacggcgt
     8401 cgatcggcag cgggcgcccg agcgagaagc tgctcggcct gttcgccgcg agctcgatga
     8461 cggtgacgag cggcagcacg gacgccgatc cgcaggcgag ctaccggcgc gacatgtgcc
     8521 agttcgccga cgatcgcctt tcggcagcgc aacgcgtcgc gttcgtgcat tcgctcctcg
     8581 accgcgatgt cgccgaggtg aggacgtttc tcgattcgct cgagaagtat gcggcgtcgc
     8641 tcaccccggc cgatcgggcg gacgcggccg tgtcgaaggg gctggaagcg atcgcgacgg
     8701 acgcgcacgc caaggagcgt tggctcgcat ttgcgcgcga cgccgacgaa ccggcgatgc
     8761 gcgcgcggat gatcgcgctt gccgcgcgtc tcggctggct ctcggagtcg tcgacgcagg
     8821 ccgagattgc gcgactcgtg cgcgacctgt acgcgcggaa cgagctcggc aacacggacg
     8881 tcgagctcgt gtgcacgctc aaccgtgatc acgcgctcga ttcggcgctc gccggcatca
     8941 gtgcgacgcc ccgggaccgc gacatcgcgc acgcggcgct cctcgcctgt gcgggcagcg
     9001 cctccgcgcg cgcggagctc atcgacgcat tgacgagcgg tcgcgaccgc gatgtcgggc
     9061 tcgcgcaggt ttatctcaag catcatccga tcgccgacgt cggtgacctc cgtgcggtcg
     9121 cgctgcgcat cgcgcggatg agcgacgtcg acgcgcagat ccgcgcgctc gacacgctgg
     9181 ccgagcaccg cgtctccgac cgcgaaagcc tcgaatcgct gacgcggctc tttccggtgg
     9241 cgaaaaccgt caacgtgcag cgcgccatcg ccgggatact catccggtcg gactacgagg
     9301 ccatcgcaaa gccggagctc gtgcgtgcgc tgcgggagaa gcggctcaaa tcgccggacg
     9361 ggcaggacct gatcgacgtg ctgattcgcc gcatgcaggc gtcgctgtcg cgcgcggcgt
     9421 aacgcttcac cccggtgcaa cgaggaagcc cgaacccaaa ccgacgcagg aggagagccg
     9481 catgaaactc tattaccacc ccgcatcgac cacgtgccgc ccgatcatgt tgttcgccgc
     9541 cgaaagcggt gtcaggctcg acttccagct ggtcgacatc ttcaccggcg agcaatacaa
     9601 ggagccgttc accgccgtca atccgaatca catggtgccg gtgctcgagg acggcgattt
     9661 ccgtctcacc gaaagctccg cgatcctcaa atacctcgcc gacagcatcg actcgccggc
     9721 gtatccgaag ccgctccggg cgcgcgcccg cgtcaacgaa cggatggact ggatcaacac
     9781 ccaggtgagc cgcgacttca cctatggctt cgtctacccg cagcttctgc cggtgttcaa
     9841 gcgcgccgac gccgcggtgc aggccgcgac gctcgcgtgg ggccgcgaac gcgcacgggg
     9901 ctggttgaag gtgctggacc agcacgtgct cggcccggag agccggtatc tgtgcggcga
     9961 tgcgatcacc atcgccgatt acttcgccgc accgttcatc acgctcggcg aggcgattcg
    10021 ttgcgactac tcggcctacc cgaacgtgtg ccgctggctt tcggcgatga aggcgctgcc
    10081 gagctggcgg aaggtgaacg aggccatcga cggcttcgcg gcgtcgctca aggacgcgca
    10141 gttcgaaacg ctctagcggg gctgacatgg cccgcgctga gcccgtgctt gcgcatcacg
    10201 acgtgctggc agcgctcgac atcgtcaggc gcgaggtgga agcgaaggac aggggggcgg
    10261 ccatcgccat cgccgacgca cacggcgagc tgatcgcctt cgtgcgctgc gatggcgcat
    10321 tgatcgcttc cggcacgctg gcgatcaaca aggcgttcac cgccgcacgg ctcaaccggc
    10381 cgacgcgcgg gctgggcgat acgctccgcg cgaagggaac cgatatcgcg ttctacggcg
    10441 atccgcgcta cgtcggcttc cccggcgggc tgccggtgcg catcggcggc atcgtcgcgg
    10501 gggccgtcgc ggtaagcggg ctgtccgacg acgaggacga gacgctcgcg gggcttgcca
    10561 tcgcttatct cgagggccgc gcgccggtgt cgatcagcgc ctagaacgac gccactgacg
    10621 tgtcgatgag cgcctagaac gtcgcttcga ccgcgcggat cagcgccggc gtgatctcgg
    10681 gcagcacgcc gaaccacgcg tggaacgcgg gccgcgcctg gttcagcagc atgccgagtc
    10741 cattgaccgt cgcattgccg cgcaggcgtg ccgcgcgcag cagcggcgtc tcgggcggga
    10801 cgtagatcag gtcgccgacg atcgccgtcg acgaaagcgg cgcgaggtcg aggtcgagcg
    10861 gcggctgtcc gatcatgccc tggttggttg cgttcacgag cagtgcgatg tcgccgagcg
    10921 tcgcgtcgcg atcgtcccag cgcaccgcga cgagcggcgg cccgaagcta tcctgcagtg
    10981 acttcgcacg ctcccaggtt cggttgacga cacggatttc gcgcgcgcct tgcgccgcaa
    11041 ggctcgcgat caccgcgcgc gccgcgccgc cggcgcccac gacggcaatc ggcccgacat
    11101 cggggcgcca gcccgggcgc gcatcgcgaa tgctctgcac gaagccgttg ccgtcgttgt
    11161 tgaatccgcg cagcgagcca tcttcctgca cgacgatcgt gttgaccgcg ccgatccttt
    11221 gcgcgagcgg atcgacgtca tcgagcagcg gcatcaccgc ctgcttgtgc ggcgtggtga
    11281 cattgcagcc acgaagccgc agcgccgaaa ggcccttcac cgcggcacgc agatcgtcgg
    11341 gcgcgacgcc gaacagcacg tagcgcccgg gcaggcgatg ctccgcgatc cagtgattgt
    11401 gaatcaccgg cgagcgcgag tgcgcgaccg gccacccgag gaggccggcg atgccgaatg
    11461 cgtgcggtga ttgcatcgcg cgcgccgcgc cgccgaggct atttcgccga tgcggcgggc
    11521 aacgtcgccg tgaccacctg cttgccgttc tcgaccttcg tcatgaagct ttcgcggtcg
    11581 agatcgccgt tctggtcgaa cgacacatcg agcaggacac cggggtgctc ctttgccgac
    11641 aggtgcaggc catgcatcgc cttggcgaag gccttctggt cgaacttgcc gatcttctcg
    11701 gtgacggcct tcacgacgta catcgcgctg taacccttga ggccgttatg gtccgacttg
    11761 gtgtggtatt ccttctggaa tttctgatcg aatgcgtgga tcgccggttg cggcgcatcg
    11821 gccgtcaatc ccacgtgcgc cgtggcgccg ttcgccgcgt cgcccgcgag ctcgatcacc
    11881 ttctggctcg tcagtaccgt ttccccgacg atcggcttgt cgtagccctg cttgcgcagc
    11941 tcacgcagcg cgcgcgccgc ttcctcttcg ttcgtgtaga cgaacagcgc atcggcattc
    12001 gcctgcttcg ccttcagcac ggcgcccgag aagtcgagct gtcccgggtc ggtggagatt
    12061 tccgccgcga ccttgatgcc ctgcgcgtcg agcgccttct tcatcgtgtc gagcccgccc
    12121 ttgccgaagt cgttgttgac ccagatgatc gcgacgttct tcgccttcag cgtgtctttc
    12181 aggtagcgcg cgaccttcgg catcgccgtc gtttgcgtga acgacgtgcg aaagatgtag
    12241 ggattgccct gctgcgtaat ggccgcggct tcgccgccgg tgaagttcgg tacctcggcg
    12301 cggcgcgtct cggccatgct gacgaggatg gagccggaaa acaccggtcc catcacgacg
    12361 taggcgccct ggtcgatcgc cttctgcgcg agcgccttcg ccacctgcgg ctgcgactgc
    12421 gtgtcgagcg acgtgtattc gatcttgcgg ccgaggatgc cgccgctcgc gttgatctcc
    12481 ttgaccgcga gcttgacgcc gttgtcgaac atcgtgcccg acgtggttcc cgtgccggac
    12541 aattcgacca ggccgaagat cggcaccgga ccctgctgcg ccgaaacgag cggcgccgtc
    12601 atgccaagcg ccagcgcgag cagccatgaa atggccttca tcaatgtcct cctcaggcgg
    12661 tggaagttac cgcgaccgcg aatctagtcg cgcggggccg aatgcgcaag tgatccgggg
    12721 tcgcggggca cgtcctcgaa cggcaggccg acgtagtttt cggccatcgc ggtcgacgcg
    12781 gccttcgaag cgaccaggta ctcgagctcg gcctgctgca gcttccgccc gatggcgccc
    12841 gtgtcgggga atcggtgcgt gagtgatgtg aaccaccacg aaaagcgctc ggccttccag
    12901 atcctgcgca ggcagatgtc agaatagtga tcgatgccgg cgctgcttcg ttcggtcaac
    12961 gcctcgatca acgcgcgcga caggaagcga acgtccgcaa tggccaggtt gagccccttc
    13021 gcaccggtcg gcgggacgat gtgcgcggcg tcgcccgcaa gaaacaggcg cccgaagcgc
    13081 atcggctcgg cgacgaagct tcgcaatggc gcgatgctct tttcgaccga ggggccggtg
    13141 accatcgtct cggctgcctc gcgatcgagg cgccggcgca gctcgtccca gaatgcgtcg
    13201 tcgctccatg catcgacgct gtcgtcgagc gcgcactgga tgtagtagcg cgtacgcgtc
    13261 ggggagcgca tcgagcacag cgcgaagccc cgcgcgtgat tcgagtagat gagctcgtgc
    13321 gataccggtg gcgtgtcggc gagaatgccg agccagccga acggatacac gcgctcgtaa
    13381 gtcgcaaggc tcgattcggg cacgctcgcg cggctgacgc cgtggtagcc gtcgcaaccg
    13441 gcgatgaagt cgcacgcgag ccgatgctcg gttccgtcgc tgcgccatgt gacaccgggc
    13501 gacgcagcgt cgaaatcgtg cggcgtgacg cggtcggctg cgtagatcgt cgtcccgccg
    13561 aatgcccggc gcgcatccat caggtcgtgc gtgacctcgg tttggccgta gatcgtcacc
    13621 tgtttcccag tgagcgaccg gaagtcgatg cgatggcgtg cgccgccgaa cgagagctcg
    13681 atgccgtcgt gcggcaggcc ttcgttgcgc atgcgcgcac cgactcctgc tgcttcgagg
    13741 aggtcgacgc tcgtctgctc gaggacgccc gcgcgaacgc ggccgagcac gtacgcagcg
    13801 ctgcgccgct cgagcacgac attgtcgatg ccggcgttgt ggagcagctg tccgagcagg
    13861 aggcccgaag gccctgcgcc gatgatcgcg acctgcgtgc ggaggggcgc cattcggcgt
    13921 gatgatgacg cgggacggcg gtgctcggca agcgcgatgc accgcatttg cgccgacgtg
    13981 cttctcgccc aatgccccgg gtgcttgacc gcatggccga tttccccgat cgtgtgccgc
//
LOCUS       DASSVX010000189         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_474393, whole genome shotgun sequence.
ACCESSION   DASSVX010000189 DASSVX010000000
VERSION     DASSVX010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474393"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1915
                     /locus_tag="VFO53_08040"
     CDS             <1..1915
                     /locus_tag="VFO53_08040"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8876262.1"
                     /translation="RIRASLAIEPKSPDAWSNLALVLNSAGRREAAANALREAATLAP
                     SSPEILANLAAAELALGRADAAEALARRAIAADASHAPAWYNLALALEPQGRLLEALD
                     ASSRATAQAPAEPAYAGFKAQLEATAGMRRKARATLDSALLRKPDSAALQFQLAGLLE
                     RDNDTSGAMQAYANVVRLDPRHGAALSQLLFLRQRVGDWHDLGALRSQFHDGVAAGTP
                     LLSPFVLLSQPSSRHEQLRCANAWTAALAPAGEPAPLRELSGHRLRIGYLSGDLHTHA
                     TAYLTAGLFEAHDRSRFEIVAYSTGPDDRSAMRQRLMRGFDRFVDAAGWPALRLAQAI
                     RNDGIDILVDLKGHTEGAAPAVLALRPAPIQVHYLGYPGTLGGNLVDYLIGDAVVTPA
                     AHAGEYSETLVRLPHSYQINDRARPIADAIPTRRELGFATSTIVLCCFNRGYKLNPEV
                     FDAWMRILAEVPDAVLWLLSRPGEETLERNLRREMVRRGLDARRLQFTAARSNPDYLA
                     LYRVADLFLDTWPYNAHTTASDALWAGCPVLTLRGETFASRVAASLLTAAGLTELITT
                     DVDAYVNKAVALASDANERQRLRAFLDGPGRGSPLFDTMATTRALEAAYDAMASQYRN
                     RTRAPIEIDALQP"
     gene            complement(1959..>3116)
                     /gene="radA"
                     /locus_tag="VFO53_08045"
     CDS             complement(1959..>3116)
                     /gene="radA"
                     /locus_tag="VFO53_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET8876263.1"
                     /translation="SRIATGLDEFDRVLGGGLVPGGVILLGGDPGIGKSTLLLQAGGA
                     LGALHRTLYVTGEESAEQIALRAQRLGLVNAPIELLAEVQLEAIVAAINATAPEIVVI
                     DSVQTVYTEALASAPGSVSQVRECAAQLTRLAKQRGVIVLFVGHVTKEGAIAGPRVLE
                     HIVDTVLYFEGDPHSSFRLVRAIKNRFGAANELGVFAMTERGLKGVANPSALFLSQHA
                     EGVAGSAVVATLEGSRPLLVEVQALVDPVQGGMPRRLAVGLDPQRLALLLAVLHRHGG
                     VDTGGFDVFVNAVGGVRILEPAADLAVLVSVCSSLKNKPVPQKTLVFGEVGLAGEIRP
                     VQRGQERIREAAKLGFKTFVIPYLNKPKQLIDDTRIVAVHRVDEALALLRE"
BASE COUNT          530 a         1060 c         1012 g          514 t
ORIGIN      
        1 gcgcattcgt gcatcgctcg cgatcgagcc gaagtcgccc gatgcgtggt cgaatctcgc
       61 actcgttctg aactcggcag ggcggcgcga ggcggccgcg aatgcgttgc gcgaggcagc
      121 aacgctcgcg ccgtcgtcgc ccgagatcct ggcgaatctt gcggcggccg aattggcact
      181 ggggcgcgcg gatgctgcgg aagcgctcgc gcgccgcgcg atcgcggccg atgcgtcgca
      241 cgcgccggcc tggtacaacc ttgcattggc actcgagccg cagggccgat tgctggaagc
      301 gctcgacgca tcgtcgcgtg caaccgcgca ggcaccggcg gagcccgcgt atgcgggttt
      361 caaggcacag ctcgaggcca ccgccggcat gcgccgcaaa gcgcgcgcaa cgctcgactc
      421 ggcgctgctg cgcaagcccg attcggcggc gctgcaattc cagcttgccg gattgctcga
      481 gcgcgacaac gatacgagcg gcgcgatgca ggcctacgcg aacgtcgtgc ggctcgaccc
      541 gcgtcacggc gcggcgctct cgcagcttct gttcctgcgt cagcgcgtcg gcgactggca
      601 cgatcttggc gcgctgcgct cgcaattcca cgacggcgtc gcggcgggaa caccgctctt
      661 gtcgccattc gtgctgctct cgcagccgtc gtcgcgacac gagcagcttc gctgtgcgaa
      721 cgcatggacc gcggcgctcg caccggcggg tgagccggca ccgctgcgcg agctttcagg
      781 ccataggctg cgcatcggct acctgtccgg cgacctgcac acgcacgcga cggcgtatct
      841 gaccgccgga ctgttcgaag cgcatgatcg ctcgcgcttc gagatcgtcg cgtattcgac
      901 cggacccgat gatcgcagcg cgatgcgcca gcgactaatg cgcggcttcg atcgcttcgt
      961 cgatgccgcc ggctggccgg cgctgcggct cgcgcaggcg atccgcaacg atggcatcga
     1021 catcctcgtc gacctcaagg gacacaccga aggcgcggca cccgcggtgc ttgcgctgcg
     1081 gccggccccg attcaggttc actacctcgg ctatccggga acgctcggcg gcaacctcgt
     1141 cgactacctg atcggcgatg cggtcgtcac gccggcggcg cacgccggcg agtattcgga
     1201 aacactcgtt cggctgccgc acagctacca gatcaatgac cgtgcgcgtc cgatcgccga
     1261 tgcgattccg acgcggcgcg agctcggctt tgcgacgagc acgatcgtgc tctgttgctt
     1321 caaccgcggc tacaagctga acccggaagt cttcgacgcg tggatgagaa tcctggccga
     1381 agtccccgat gcggtgttgt ggctgctctc gcgtccgggc gaggaaacgc tcgagcgcaa
     1441 cctgcggcgc gagatggttc gtcgcggact cgatgcgcga cggttgcagt tcaccgccgc
     1501 gcgcagcaat cccgactatc ttgcgctcta tcgcgtcgcc gacctcttcc tcgacacgtg
     1561 gccttacaac gcgcacacga cggcgagcga cgcattgtgg gccggctgcc ccgtgctgac
     1621 gttgcgcggt gaaacgttcg cctcgcgcgt tgcggcaagc ctgttgaccg cggcaggcct
     1681 gacggaattg atcaccaccg acgtggacgc ctacgtgaac aaggcagtgg cgctcgcaag
     1741 cgacgcgaac gagcggcagc gcttgcgggc cttcctcgac ggcccgggtc gcggcagtcc
     1801 gttgttcgat acgatggcca cgacgcgcgc gctcgaggcc gcgtatgacg cgatggcgtc
     1861 gcaataccgt aaccgcacgc gggcgccgat cgaaatcgac gcgttgcagc cgtagccgac
     1921 atcattcggc cgcggccgta ggtgcgcgaa atcttgcgct actcgcggag cagcgccagt
     1981 gcctcgtcca cgcggtggac ggcaactatg cgcgtgtcgt caatgagttg cttcggttta
     2041 ttaaggtagg gtatcacgaa cgtcttgaag ccaagcttgg cggcttcgcg gatccgctcc
     2101 tggccgcgct gaaccggacg aatttcaccg gcaagtccga cttcaccgaa cacgagagtt
     2161 ttctgcggta caggtttgtt tttcaaagat gaacacaccg atacgagcac ggcgagatcg
     2221 gctgccggct ccagtatcct cacgccgccg acggcattga caaagacatc gaagccgccg
     2281 gtatccacac cgccgtgacg atgcagtacg gcgagcaaca gcgcgagccg ctgcgggtcg
     2341 agcccgacgg cgagccgacg cggcatgccg ccctggacgg gatcaacgag cgcctgcact
     2401 tcgacgagca gcgggcgcga cccttcgagc gttgcgacga ccgccgaccc ggcaacgcct
     2461 tcggcgtgct gcgacaggaa caacgccgat ggattggcga cgcctttgag gccacgctcg
     2521 gtcatcgcga acacgccaag ctcgttggca gcgccaaaac gattcttgat cgcgcgcacg
     2581 agccgaaagc tcgaatgcgg atcgccttcg aagtacagca ccgtatcgac gatgtgctcg
     2641 agcacgcgcg ggccggcgat cgcgccctct ttcgtcacgt gaccgacgaa gagcacgatg
     2701 acgccgcgct gtttcgcaag gcgcgtcaat tgcgccgcac actcgcgcac ctgcgagaca
     2761 ctgccgggtg ccgacgcgag cgcctcggtg taaacggtct gcaccgagtc gatgacgaca
     2821 atctcgggcg cggttgcatt gatcgccgca acgatcgcct cgagctgcac ttccgcgaga
     2881 agctcgatcg gtgcgttgac gagcccgagc cgttgcgcgc gaagcgcgat ctgctcggcc
     2941 gattcctcgc cggtcacgta gagtgtccga tgcagcgctc cgagtgctcc acctgcctgc
     3001 agcagcagcg tggacttgcc aattcccgga tcgccaccga gcaggatcac gccccctggc
     3061 accaggcctc cgcctagcac gcggtcgaac tcgtcgagcc cggttgcgat acgtga
//
LOCUS       DASSVX010000190         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_707689, whole genome shotgun sequence.
ACCESSION   DASSVX010000190 DASSVX010000000
VERSION     DASSVX010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707689"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFO53_08050"
     CDS             complement(<1..205)
                     /locus_tag="VFO53_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HET8876264.1"
                     /translation="MSRNSRNPAFRKAVLPALVAAAVAACWAAPAAASSHREAPFIAT
                     QPQVDATDFYMFRSYEPGREGYVT"
     gene            416..1075
                     /locus_tag="VFO53_08055"
     CDS             416..1075
                     /locus_tag="VFO53_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8876265.1"
                     /translation="MRILLVEDDPMIGKTLQQALQQDGYAVDWVTDGESGRTAIATSQ
                     DAYVLVLLDLGLPRKGGLALLRELRREGNRIRVLVVTARDAVADRVEGLDAGADDYLT
                     KPFSLDELTARIRALLRRDVAREDNVLRHGDLTLDTASHAVTKAGKAVDVSAREFALL
                     AALLERPGTALSKAQLEERIYRWGDEVESNAVEVHIHNLRKKLGSDAIRTLRGVGYTL
                     A"
     gene            1072..2439
                     /locus_tag="VFO53_08060"
     CDS             1072..2439
                     /locus_tag="VFO53_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8876266.1"
                     /translation="MNSIRRTLLAWLLAGLAAIALTASGATYLAARREVGELLDLQLK
                     QLAYSTRIDDLLRGRRPGIEPRTAPSSTGITELVTQIWDRDGVLVYWTQPGMGLPVPA
                     TEGYSTANHDGRRWRVYTLVQGTHALQVAEAESEREAIATQTALRTLVPFIALVPLFG
                     ALIWFGVGRGLAPLDAISRAVAKRRADAMAPLNERGVPEELQPLVVSLNGLLARLSEA
                     LAAQRRFTADAAHELRTPLAALKLQLDLARRGGTASGASAFDDLDAGVARASHVVEQL
                     LTLARVEPEALAQQRSDCDLVALARDAIVARAALAADKAIDLGLARAEAAIVSGDPAS
                     LAILLSNLIDNALRYTPRGGRIDVAVDRDESGATLTVTDNGPGIPTEDRARVFDRFFR
                     GNDQHEQGSGLGLSIVKRIADAHHASIALDDAPGGGLAVRVHFPHVAEAGEPVAKPGE
                     QADFS"
BASE COUNT          435 a          878 c          900 g          432 t
ORIGIN      
        1 tcgtcacgta accttcgcgg ccgggctcgt agctgcggaa catgtagaag tcggtcgcgt
       61 cgacctgagg ctgggtcgcg atgaacggcg cctcgcggtg gctcgatgca gccgcggggg
      121 cggcccagca cgcggcaacg gcggcggcga cgagcgccgg caggacagct ttgcggaatg
      181 cgggattgcg gctgttgcgt gacattacgg tctcctcgga cgtttgaact cgaaacgggg
      241 cgcaatcgca ccgtttcccg cacgaccggc aacctacccg gtcggtcggc ggttacgaat
      301 tacctccgca caggaggcca tacggcggat cggcggattt ggattcacgc tcagcagcac
      361 ttaaggttcg gcccgtagaa tcggccggtt ctcctggaag gatccgagcg ccgcgatgcg
      421 catcctgctc gtcgaagacg acccgatgat cggcaagacg ctgcagcagg cgctgcagca
      481 ggacggttac gccgtcgact gggtcaccga cggcgagtcg gggcgcacgg cgatcgcaac
      541 ttcgcaggac gcctacgtgc tggtgctgct cgacctcggc ctgccgcgca agggcggcct
      601 cgcgctattg cgggagctgc ggcgggaagg caaccggatt cgcgtgctcg tcgtcaccgc
      661 gcgcgatgcg gtcgccgatc gtgtcgaagg gctcgatgcc ggcgccgatg attatctgac
      721 caagccgttc agcctggacg agctgacggc gaggatccgc gcgctgctgc gccgcgacgt
      781 cgcgcgcgag gacaacgtgc tgcgccacgg cgatctcacg ctcgacacgg ccagccacgc
      841 cgtcaccaaa gccggcaagg cagtcgacgt gtcggcgcgc gaattcgcgt tgctcgctgc
      901 attgctggag cgacccggca ccgcattgtc gaaggcgcag ctggaggagc gcatctatcg
      961 gtggggcgat gaggtcgaga gcaatgccgt cgaagtgcac attcacaact tgcgcaagaa
     1021 gctcggcagc gacgcgatcc gcacgctgcg tggcgtcggc tatacgctcg catgaactcg
     1081 atccgccgca ccctgctcgc gtggctgctg gcgggcctcg cggcgatcgc gctgaccgcg
     1141 agtggggcaa cctatctcgc agcgcggcgt gaagtcggcg agctgctcga cctgcagctg
     1201 aagcagctcg cgtattcgac gcggatcgac gatctcttgc gcggacggcg tccgggcatc
     1261 gagccgcgca cggcgccttc gagcaccggt atcactgagc tcgtcacgca gatctgggat
     1321 cgcgatggcg tgctcgtcta ctggacgcag cccggcatgg gattgccggt gcccgcgacc
     1381 gaaggttatt cgacggcgaa tcacgatggt cggcgctggc gcgtctatac gctcgtgcag
     1441 ggcacgcatg cgctgcaggt ggccgaggcc gaaagcgagc gtgaggcgat cgcaacgcaa
     1501 acggcgctgc gcacgctcgt gcccttcatc gccctcgtgc cgctgttcgg tgcgctgatc
     1561 tggtttggtg tcgggcgcgg tctcgcgccg ctcgacgcga tctcccgcgc tgtcgccaag
     1621 cgtcgcgccg acgcgatggc gccgctcaac gagcgcggcg ttcccgagga actgcagccg
     1681 ctcgtcgtca gcttgaatgg attgcttgcg cggctgtcgg aggcgctcgc agcccagcgg
     1741 cggttcaccg ccgacgcggc acacgagctg cgcacgccgc tcgcggcgct caaattgcag
     1801 ctcgatctcg cgcggcgcgg cggtaccgcc agcggtgcat cggcattcga cgatctcgat
     1861 gcgggcgtcg cacgcgcgtc acacgtcgtc gagcaactgc tgacgcttgc gcgcgtcgaa
     1921 ccagaagcgc tggcgcaaca gcgcagcgac tgcgatctcg tcgcactcgc gcgcgatgcg
     1981 atcgtcgcgc gtgcagcgct cgccgcagac aaggcaatcg atctcgggct cgcgcgggcg
     2041 gaagccgcca ttgtcagcgg tgatccggcg agccttgcga tcctgctatc gaacctgatc
     2101 gacaatgcgc tgcgttacac gccgcgcggc ggacgcatcg acgttgcagt cgatcgcgac
     2161 gaaagcggcg cgacacttac ggtgaccgat aacggtccgg ggattccgac cgaggaccgt
     2221 gcccgcgtgt tcgaccgctt tttccgcggc aacgatcagc acgagcaagg cagcggtctc
     2281 ggcttgtcga tcgtcaagcg catcgccgat gcacatcacg cgtcgatcgc gctcgacgat
     2341 gcgccgggcg gcggcctcgc cgtccgcgtg catttcccgc acgtggccga agcgggagaa
     2401 cccgtcgcaa aaccaggcga gcaggccgac ttctcttaag gtgcggttaa ggaggagatc
     2461 ctatattgca tcgtgccggc cggactttcg agcggccccg acgtcccacc caggagacac
     2521 gaatgcaacc gacccttcgt ttcaaacaga tcaccgtcgc agtcgccgct gccggcttcg
     2581 gccttgccgt cggcgccggc tacaaccgcc ttgacacgca cgccgttgcg ctggcgaatg
     2641 ccgct
//
LOCUS       DASSVX010000191         3684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1041047, whole genome shotgun sequence.
ACCESSION   DASSVX010000191 DASSVX010000000
VERSION     DASSVX010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041047"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1235)
                     /locus_tag="VFO53_08065"
     CDS             complement(174..1235)
                     /locus_tag="VFO53_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009394257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8876267.1"
                     /translation="MTRILRVAVVGAGRIGGRHIEEIRALPGAALSAIVDVSAAAAAL
                     AQQAGVPLFASIDELLARDRPDGIVLATPNALHAAQALACIDARVPALVEKPIAHTLA
                     DGARIRDAAERANVPILVGHHRRHSPILRTGCEIVASGVLGRLVGVIGSAVFCKPDAY
                     FAEAAWRREPGGGPILINMIHEVDNLRAMLGEIDAVQAFSSNAARGFAVEDTVSINLR
                     FANGALGTFLLSDTGASAKSWEQTARENAAYAAYPDEDCYTIIGTQGSLGVPTMQVRC
                     HEAGAERSWYQPLATHTAAFERKDPLAVQIAHFVAVIRGEAKPLVTARDGVANLRVVE
                     AIVEAARTGRVVDTMERGR"
     gene            complement(1232..2026)
                     /gene="aroD"
                     /locus_tag="VFO53_08070"
     CDS             complement(1232..2026)
                     /gene="aroD"
                     /locus_tag="VFO53_08070"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET8876268.1"
                     /translation="MKPLVVNGSPVADGRLPAICVPLVGHTTEAIDAELAAVMQKSPD
                     VLEWRVDHFADVANAGAVIDVAHRIRAAADGTPLIFTRRSEREGGAQTALGDEQAVAL
                     YEAVCAARCAQFVDWELSGGPASLARVRAAAHANGVAVIASFHDFRATPATSVLLGKF
                     ERAAREGADVAKVAVMPNEPRDVLTLLDATWQASRSLDVALISMSMGPLGVLSRIAGF
                     LYGSALTFGVGAGSSAPGQLAIDELRTSLETMRRASATVAAGRSKP"
     gene            complement(2106..>3684)
                     /locus_tag="VFO53_08075"
     CDS             complement(2106..>3684)
                     /locus_tag="VFO53_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876269.1"
                     /translation="GGLGGPQVVRPHSVVELTIQPAAGGAAKAVRLEATRTTRSWGDR
                     LVLLADTIVGIVVILAACRLVWLRPGAMTWGFFLYVMWFNPGQTFAYYAVLQPWPVAI
                     FVQEIAEALAHGAGYAGLLIFALCFPFGSPRPAMRRYERIALGIGAAITLTWLASFSN
                     AFGAHTETLTSLAFLLGYALDAIVVFILVRRRHALPPPDRQRMIWVICGCAIGLPAFI
                     FAEIAQSTSLLQNALGLSLSNVVVGLLYLVNGVLVYFVSVAVLRRRVVSVSIPLRYGT
                     ILTVLSLAVGIPIVNLHELLSHYQGSFRIPEWIWLLVIAPITLVLLQRLHELGVKLVD
                     RLLNRRFHAARRRLEVAGEAMLTAQRFEEIDRLLVATVVGALQLSSGAAFREDNGAFR
                     RVFAVGWDASQASTLAPERDAEALQSIVRRVPVRLQRTEWDGPDVVAKLRDPCIAVPV
                     VSDVLGAVGIALFGAHENGNDIDADECEMLVEFARRAAAGYERAAFVMLREEVADFRS
                     RLGAPQKRPPETAPRPA"
BASE COUNT          645 a         1279 c         1227 g          533 t
ORIGIN      
        1 cctctcgtgt cagtggatat gtccacattt ccactgtggc actctgatgc cgcatcggga
       61 agggggcgac catcccatta gcgtctttcg ctcgaacgcg ccactgggtc cccgccttcg
      121 cggggacgac gaagcttcgg gagcccgacg tcacgctatg taaggctcca tagtcagcgg
      181 ccgcgttcca tggtatcgac gacacggccg gtccgtgccg cttcgacgat cgcctcgacg
      241 acgcgcaggt ttgcaacgcc gtcgcgcgcc gtgacgagcg gcttggcctc gccgcgaatc
      301 accgcgacga aatgtgcgat ctgcacggcc agcgggtcct tccgctcgaa cgccgccgta
      361 tgcgtagcga gcggctggta ccacgagcgc tcggcccccg cttcgtggca tcgcacctgc
      421 atcgtcggca cgccgagcga accctgcgtg ccgatgatcg tgtagcaatc ctcgtccgga
      481 taggcggcgt acgcggcatt ctcgcgcgcc gtctgttccc agcttttcgc agacgccccg
      541 gtatccgaca gcaggaacgt gccgagcgcg ccgttggcga agcgcaggtt gatcgacacc
      601 gtgtcctcga cggcgaaacc gcgcgccgca ttcgacgaga acgcctgtac ggcgtcgatc
      661 tcgccgagca tcgcccgcag attgtcgact tcgtgaatca tgttgatcag gatcggtccg
      721 ccgcccggct cgcgacgcca ggccgcctcg gcgaaatagg catcgggctt gcagaagacc
      781 gcgctgccga tcacgccgac cagccgcccg agcacgcccg acgcgacgat ctcgcagccc
      841 gtacgcagaa tcggactgtg cctgcgatga tggccgacga ggatcggcac gttcgcgcgc
      901 tcggcggcgt cgcggatgcg cgcaccgtcg gcaagcgtat gtgcaatggg cttttcgacg
      961 agtgcgggta cgcgcgcgtc gatgcacgcg agcgcctgcg cggcatgcaa cgcgttgggc
     1021 gtcgccaaca cgataccgtc gggccggtcg cgcgcaagca attcgtcgat tgaagcgaaa
     1081 agcggcacgc ccgcttgctg cgcaagcgcg gccgcagccg cgctgacatc gacgatggcg
     1141 ctgagtgctg cgcccggcag cgcgcggatt tcctcgatgt ggcgcccgcc gatgcgcccg
     1201 gcgcccacga cggcgacacg caggatgcgc gtcacggctt cgatcggccg gcagccaccg
     1261 tcgcgctcgc gcggcgcatc gtttcgagcg aggtgcgcaa ctcgtcgatc gcgagctgcc
     1321 cgggcgccga gctgcccgcg ccgacgccga atgtgagcgc cgatccgtac aaaaaacccg
     1381 cgatgcgcga cagcacgccg agcgggccca tcgacatgct gatcagcgcg acgtcgagcg
     1441 agcggctcgc ctgccatgtc gcgtcgagca gggtcagcac atcgcgcggt tcattgggca
     1501 tcacggcgac ctttgcgacg tcggcgcctt cgcgtgccgc ccgctcgaat tttccgagca
     1561 gaacgctcgt tgccggcgtc gcacggaaat cgtggaacga cgcaatcacg gcgacgccgt
     1621 tcgcatgcgc cgcggcgcgc acgcgcgcaa ggctcgcggg tccgcccgaa agctcccaat
     1681 cgacgaactg cgcacagcgc gccgcgcaca ccgcctcgta caatgcgacc gcctgctcgt
     1741 cgccgagcgc cgtttgcgcg ccgccttcgc gctcggaacg gcgcgtgaag atcagcggtg
     1801 tgccgtccgc ggcggcccgg atgcgatgcg ccacgtcgat gacggcgccg gcgttcgcga
     1861 catccgcgaa atggtcgacg cgccactcga gcacgtcggg cgacttctgc atgaccgcgg
     1921 caagctcggc gtcgatcgcc tcggtcgtgt ggcccacgag cggcacgcaa atcgccggca
     1981 gccggccgtc cgcgaccggc gacccgttca cgacgagtgg cttcacgagg tgcaaacgag
     2041 ccccgcgctt cgtgcgatca aggaggtccg gcggtacatt ggctgcaacg ataaacgaat
     2101 cgaggtcacg ccgggcgtgg cgcggtttcg ggtggccgtt tctgcggggc accaaggcgg
     2161 cttcgaaaat cggccacctc ctcgcgcagc atgacgaacg ccgcgcgctc gtatcccgca
     2221 gcggcgcgcc gcgcgaactc gacgagcatc tcgcactcat cggcgtcgat gtcattgccg
     2281 ttctcatgcg caccgaaaag cgcgatgccg actgcgccga gcacgtcgct gacgacgggc
     2341 accgcgatgc atggatcgcg cagctttgct acgacatccg gaccatccca ctcggtgcgc
     2401 tgcaggcgaa ccggtacccg ccgcacgatg ctttgcagtg cctcggcatc gcgttcgggt
     2461 gcaagcgtgc tcgcttgcga cgcgtcccac ccgaccgcga acacgcggcg gaacgccccg
     2521 ttgtcctcgc ggaacgccgc gccggacgac agttgcagcg cgccgacgac ggtggccacg
     2581 agcaggcggt cgatctcctc gaagcgctgc gccgtgagca tcgcctcgcc cgcaacctcg
     2641 aggcggcgac gcgccgcgtg gaagcggcgg ttgagcaggc ggtcgacgag cttgacgccg
     2701 agctcgtgca gacgctgcag cagcacgagc gtgatcggcg cgatcaccag cagccagatc
     2761 cattccggga tgcgaaagct tccctggtag tgggacagaa gctcgtgcag gttgacgatc
     2821 ggtatcccta cggcgagcga cagcacggtc agtatcgtgc cgtagcgcaa cggaatcgaa
     2881 acgctgacca cgcgccggcg gagcacggcc accgacacga agtagacgag cacgccgttc
     2941 acgaggtaga gcagccccac gacgacgttc gacaaggaca agccaagcgc attttgcaga
     3001 agcgacgtcg attgcgcgat ctccgcgaag atgaacgccg gcagcccgat cgcacagccg
     3061 cagatcaccc agatcatccg ctgccgatcg ggcggcggca gcgcatggcg gcgacgcacg
     3121 aggatgaaca cgacgatcgc gtcgagcgcg tagccgagca ggaacgcgag cgacgtcagc
     3181 gtttcggtgt gcgcgccgaa cgcgttgctg aagcttgcga gccacgtcag ggtgatggcg
     3241 gcgccgatgc cgagcgcaat gcgttcgtag cgacgcatcg cgggacgcgg ggaaccgaat
     3301 ggaaagcaca gcgcgaagat cagcaggccc gcatagccgg cgccgtgcgc gagcgcctcg
     3361 gcgatttcct gcacgaaaat cgcgaccggc cagggctgca gcacggcgta gtacgcaaac
     3421 gtctgcccgg ggttgaacca catcacatag aggaagaatc cccacgtcat tgcgccgggg
     3481 cgaagccaga cgagcctgca tgcggcgagg atcacgacga tgcccacgat cgtgtcggcc
     3541 agcagcacga gcctgtcacc ccagctgcgc gtggtgcgcg tcgcctcgag ccgcaccgct
     3601 ttcgccgcac cgccagcggc gggctggatc gtgagctcga cgacgctgtg cggccgcacg
     3661 acctgcggcc cgcccaggcc gccg
//
LOCUS       DASSVX010000192         3903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1607799, whole genome shotgun sequence.
ACCESSION   DASSVX010000192 DASSVX010000000
VERSION     DASSVX010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607799"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFO53_08080"
     CDS             <1..909
                     /locus_tag="VFO53_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HET8876270.1"
                     /translation="RGALVVADALALVAAWRAAIALSFEGFRANLSPLDPRAVAARPA
                     PGQVDVAAQLRALLDGSALLAPGAARRVQDPMSFRVVAQVHGSLQTALERAREQVELE
                     LDSAAESPLVLAHDAIMLSNGNFHLPAFTLAFDALAIAFAQGASLAAGRVIRFMSPAF
                     TGLPLQLTRHGPAHSGFATLQKTLTVLWSGIRMQANPASLDFLPISDDVEDHATMALS
                     TVERVGEMVDRLRYIVAIEMLVAAQAIDLRELDARQLGVGPRTLHARIREHVPMLAHD
                     RPLGPDVDTIAALIGSPDFSATAPGP"
     gene            906..1529
                     /locus_tag="VFO53_08085"
     CDS             906..1529
                     /locus_tag="VFO53_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876271.1"
                     /translation="MSWFLRNADQVAFALGQHVVIALTALAIAFAIALPVGIVAARRE
                     RVHAIAIAVAGFLYTIPTLAFLALLIPLVGLGPANAIIAMVAFSLVVLIRNIATGIRG
                     VAPDVVDAARGMGMSAPQLLLRVELPLALPVIVAGLRVAAVTVISVTVVAAYVNAGGL
                     GTLIFNGIATDHAPKIWSGALTACALAIAVDGALALLERRLVRTRSG"
     gene            1534..2175
                     /locus_tag="VFO53_08090"
     CDS             1534..2175
                     /locus_tag="VFO53_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876272.1"
                     /translation="MLVDAWHYLVGHPAQFGVALGVHVALSVAALSIAAGIAIPAGIY
                     AAHRRGVAFAAVNVANIGRTLPSLAVLALVLPVLGTGFAPSLFALSLLALPPILLNTI
                     AGMRGVDPDVVDAARGMGMTARGILRRVEWPLAVPVIFAGLRTAAIQVISGAVLAAFI
                     GGGGLGDFITAGIAMMAVPQMLVGAIPVTLLALAADFGFGALQRRLTPRGLCA"
     gene            2172..3116
                     /locus_tag="VFO53_08095"
     CDS             2172..3116
                     /locus_tag="VFO53_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876273.1"
                     /translation="MIELQHLTKRFPGSAVAAVDDLTLLIGEGEICVLIGPSGCGKTT
                     TMRMINRLIEPDGGTIRIGGRDVTGSDPVQLRRSVGYVIQQVGLFPHMTIAENVATVP
                     HLLGWDAPRIARRVDELLARVDMEPARYRDRYPRELSGGQKQRVGVARALAADPPVML
                     MDEPFGALDPITRASLQDELLRLLRELTKTIVFVTHDIDEALKMGTRIAIMRAGRLVQ
                     FDRPEAILAHPADAFVDAFVGSDRALKRLGLMAAADYATHAITPAPGAPRLSHGASLR
                     DALSTLLAANQDAAVTHGANGTAGVITLGTIRAALHAR"
     gene            3431..3826
                     /locus_tag="VFO53_08100"
     CDS             3431..3826
                     /locus_tag="VFO53_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876274.1"
                     /translation="MLSTKLAAIFIRRFSPYSRFTILFGLRVQSPEEWQSKVLAVSIL
                     NIAGCHQALHFFNVIRAFRQEPAPAYILELGPRSFKKISGQRRLLFFQRHLTPAAGTL
                     GAFSNRQSDPSRMRRFSRIAAASVNAGIP"
BASE COUNT          563 a         1347 c         1249 g          744 t
ORIGIN      
        1 cgcggcgcgc ttgtcgtcgc cgacgcgctc gcgctcgtgg ccgcctggcg tgcggccatc
       61 gcgctgtcgt tcgaggggtt tcgcgccaac ctgtcaccgc tcgacccgcg cgcggttgcc
      121 gcgcggccgg cgcccgggca ggtggacgtt gccgcacagt tgcgcgcgct gctcgacggc
      181 agtgcgctcc tggcgcccgg cgccgcgcgg cgagtgcagg acccgatgag ctttcgcgtc
      241 gtggcacagg tgcacggttc gctgcagacg gcactcgaac gcgctcgcga gcaggtcgag
      301 ctcgagctcg acagcgccgc cgagagcccg ctcgtgcttg cgcacgacgc aatcatgctg
      361 tcgaacggca actttcacct gcccgcgttc acgctggcgt tcgatgcgtt ggccatcgcg
      421 tttgcgcagg gcgcgagcct tgccgccggg cgcgtgatcc gcttcatgtc gccggcattc
      481 accgggttgc cgttgcagct tacccgccac gggccggcgc attccgggtt cgcgacattg
      541 cagaagacgc tgacggtgct ctggtccggg atccggatgc aggcgaatcc ggcctcgctg
      601 gatttcctcc cgatatccga cgatgtcgag gatcacgcca ccatggcgct ttcgaccgtc
      661 gagcgagtgg gcgagatggt cgatcgcctg cgctacatcg tcgccatcga aatgctcgtt
      721 gcggcgcagg cgatcgactt gcgcgagctc gatgcacgcc aactcggcgt cggcccgcgc
      781 acgctgcatg cgcgcatccg cgagcacgtg cccatgcttg cacacgaccg cccgctcggc
      841 cccgacgtcg acacgatcgc cgccctgatc ggatcgcctg acttcagcgc gactgctccg
      901 gggccatgag ctggttcctg cgcaacgccg accaggtggc gttcgcgctt ggccagcacg
      961 tcgtcattgc gctgaccgcg cttgccatcg cattcgccat tgcgctgccg gtcggcatcg
     1021 ttgccgcgcg gcgcgagcga gtccatgcca tcgcgatcgc ggtcgccggt ttcctctaca
     1081 cgatcccgac gctcgccttc ctcgcgctgc tgatcccgct cgtcggcctc ggacccgcga
     1141 atgcgatcat cgcgatggtg gccttttcgc tcgtcgtgct gattcgcaat atcgccaccg
     1201 gcatccgcgg tgttgcgccc gatgtcgtcg acgccgcgcg cggcatgggc atgagcgcgc
     1261 cgcaactgct gctgcgcgtc gaattgccgc tcgcgttgcc ggtgatcgtc gccgggttgc
     1321 gcgtcgccgc ggtgacggtg atcagcgtca ccgtcgtggc cgcgtacgtg aacgccggcg
     1381 gactcgggac gctgatcttc aatggcatcg ccaccgatca cgccccgaag atctggtccg
     1441 gcgcgctcac cgcgtgtgcg ctggccatcg ccgtcgacgg cgcgctcgcg ctgctcgagc
     1501 ggcgcctcgt gcgtacgcgc agcggctagg gcgatgctcg tcgacgcctg gcactatctg
     1561 gtcggccacc cggcgcagtt cggcgtcgcg ctcggcgtcc atgtcgcgct gtcggtcgcc
     1621 gcactttcga tcgccgccgg catcgcgatt ccggccggca tctacgcggc ccaccgccgg
     1681 ggtgtcgcgt tcgccgcggt caatgtcgcc aacatcggtc gcacgctccc ctcgctcgcg
     1741 gtgctcgcgc tggtactgcc ggtgctcggc accggcttcg cgccgagcct gttcgcgttg
     1801 tcgctgctcg cgctgccgcc catcctgctc aacacgatcg ccggcatgcg cggcgtcgac
     1861 cccgacgtcg tcgacgcagc acgcgggatg ggcatgaccg cgcgcggcat cctgcgccgc
     1921 gtcgaatggc cgctcgccgt cccggtgatc ttcgcggggc tgcgtacggc ggcgatccag
     1981 gtgatctcgg gcgccgtgct cgccgcgttc atcggcggcg gcggcctcgg cgatttcatc
     2041 accgcgggga tcgcgatgat ggcggtgccg caaatgctcg tcggcgcgat cccggtcacg
     2101 ctgctcgcgc tcgccgccga tttcggcttc ggcgcactgc agcggcggct gacgccgcgc
     2161 gggctttgcg catgatcgag ctgcagcatc tgacgaagcg ctttcccggc agtgcggtcg
     2221 cggcggtcga cgatctcacg ctcctcatcg gcgagggcga gatctgcgtc cttattgggc
     2281 cgtcgggctg cggcaagacg acgacgatgc gaatgatcaa tcgtttgatc gagcccgacg
     2341 gcggcacgat ccgcatcggc ggacgcgacg tgaccggcag cgatcccgtg cagctccgcc
     2401 ggtcggtcgg ctacgtcatc cagcaagtgg gacttttccc gcacatgacg atcgcggaga
     2461 acgtcgcgac ggttccgcat ctgcttggct gggatgcacc gcgcatcgcc cggcgcgtcg
     2521 acgagttgct cgcacgcgtc gacatggagc cggcccgcta tcgcgatcgc tatccgcgcg
     2581 agctctcggg tggccagaaa cagcgcgttg gcgtcgcgcg cgcactcgcg gccgacccgc
     2641 ccgtgatgct gatggacgag ccgttcggcg cgctcgatcc aatcacccgc gcgtcgttgc
     2701 aggacgagtt gctgcgcctt ctgcgcgagc tcacgaagac gatcgtgttc gtgacgcatg
     2761 acatcgacga ggcgctcaag atgggaacgc gcatcgcgat catgcgtgcc ggccggctcg
     2821 tgcagttcga ccgtcccgaa gcgatcctcg cgcatccggc ggacgcattc gtcgatgcct
     2881 tcgtcggcag cgatcgtgca ttgaagcgcc tcgggctgat ggcggccgcc gattacgcca
     2941 cgcacgcgat cacgccggcg cccggcgcgc cgcggctctc ccacggcgcg agcctgcgcg
     3001 atgcgctgtc gacgctgctc gccgcgaacc aggacgccgc cgtcacgcac ggcgcaaacg
     3061 gcacggccgg cgtgatcacg ctcggtacca tccgcgccgc gctgcacgcg cgataatccg
     3121 cgcgagccgg aaagcgacga tggacgccga gaagcacacg ccgattgtgt aactagagat
     3181 acgcgtcaat caggcgaaat gcgcgttttc ccttgtttta tagtggcttg gagcacttac
     3241 gccggctctg cgccggagcg cggttttggc gtggattggc atggtttggc gatgaaatta
     3301 ccccaaaatt accccaacaa atttgctcgg aacttgaggc atatcccagc gtgtgtcggc
     3361 gtacttcgct gacacttgtc tgagcactcc gcaacacgct tagacacgca catcgcgttg
     3421 gcttccggaa ttgctgagca ccaaactggc ggccattttc atccgcagat tttcacctta
     3481 ctctcgcttc acgattctat tcggcctgcg agttcaatca cccgaggagt ggcagtctaa
     3541 ggtgcttgcc gtctcgatac tgaatatcgc tggctgccat caggcgctcc acttcttcaa
     3601 tgtcattcga gcgtttcgcc aagaacccgc gcctgcttat atcttggaac tcggcccgcg
     3661 tagctttaaa aagatctctg ggcaacgtcg tcttcttttt tttcaacgtc atttgacgcc
     3721 cgctgccggc acgctgggcg cattctctaa tcgacaatct gacccgagca gaatgcgcag
     3781 attttctcgt atagccgcag caagtgttaa tgctggcatc ccgtgaagtc gtccgagcgc
     3841 ttcaaccacc aaagtcacat ctgatggttt cgatgggcga ttggttgtcc gcgtaaacgg
     3901 gcc
//
LOCUS       DASSVX010000193         2642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_741073, whole genome shotgun sequence.
ACCESSION   DASSVX010000193 DASSVX010000000
VERSION     DASSVX010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741073"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFO53_08105"
     CDS             complement(<1..596)
                     /locus_tag="VFO53_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8876275.1"
                     /translation="MNQRDFHGRRAVVTGGAAGIGLAVAQRLAAGGARVALWDRDERA
                     LASANEKLDRAAITQRVDVTDAAAVERAAKSASSELGGLDVLVCSAGITGPNTTTWEY
                     PVDAWRQVFDVNVHGLFHCNRAAVPIMLDGGYGRIVNIASVAGKEGNPNAPAYSASKA
                     AVIGLTKSLGKELAKTGIRVNCVTPAAVKTGMFEQMTQ"
     gene            815..973
                     /locus_tag="VFO53_08110"
     CDS             815..973
                     /locus_tag="VFO53_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HET8876276.1"
                     /translation="MSVSLIALIIVILLLVGALPSWGYSSGWGYWPSGGLGLVLLILI
                     ILMVMGRI"
     gene            1147..2469
                     /gene="hisD"
                     /locus_tag="VFO53_08115"
     CDS             1147..2469
                     /gene="hisD"
                     /locus_tag="VFO53_08115"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET8876277.1"
                     /translation="MAKEVLKQASRTPESGATDTRERVERMLQDIEQRREAAVREYAA
                     KLDGWTGDFVVAPSAIAAAEASLSDEVKDDLRFAHRQVDAFARKQRESLQEFETELYP
                     GLVTGQRLIPDNVAGCYVPSGRYAHVASAIMSITTAKAAGVPHVIACSPSYRGRGIHP
                     VVLYAMALAGADIIMTLGGVQAIATLALGLFSDRPADVIAGPGNKYVAEAKRILFGRV
                     GIDVFAGPSEICVIADDSADAAIVASDLVGQAEHGVESPAWLITTSRRVASEVAERIP
                     AVIAALPSEAAAAAGAAWRDYGEIVLTDTREEAAQASDAYAAEHLEVHAQDLDWWLAR
                     LTNYGSLFLGEETTVAYGDKTSGPNHILPTRQAARYSGGLSVGKFIKTVTYQRMTRDA
                     TRRIAGVTARISRLEGMEAHARTADDRLRKYFPGESFVTMPSVNSERD"
     gene            2477..>2642
                     /locus_tag="VFO53_08120"
     CDS             2477..>2642
                     /locus_tag="VFO53_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET8876278.1"
                     /translation="MKALVYTGPNTMRYRDEPEPAAAGGEVVVEVEAVGICGSDLHAF
                     HGRDPRRVPPL"
BASE COUNT          437 a          876 c          887 g          442 t
ORIGIN      
        1 tgctgcgtca tctgctcgaa catgccggtc ttcacggccg cgggtgtcac gcagttcacg
       61 cggatgccgg tcttcgcaag ctccttgccg agcgacttcg tcaggccgat gaccgcggcc
      121 ttcgatgcgc tgtacgcagg cgcattcggg ttgccttcct tgcccgcgac cgatgcgatg
      181 ttgacgatgc gtccatagcc gccgtcgagc atgatcggca ctgctgcacg gttgcagtga
      241 aaaaggccgt gcacgttgac gtcgaacacc tgtcgccacg catcgaccgg atactcccac
      301 gtggtcgtgt tcgggccggt gatgcccgcc gagcacacca gaacgtcgag gccgccgagc
      361 tcgctcgacg cggacttcgc ggcgcgctcg accgcggccg cgtcggtgac gtcgacccgc
      421 tgcgtaatgg cggcgcgatc gagcttctcg ttggcgctcg cgagcgctcg ctcgtcgcga
      481 tcccacaacg ccacccgcgc gccgccggcg gcaagccgtt gcgcgaccgc gagtccgatg
      541 cccgcggcgc cgcccgtgac gacggcacga cggccgtgaa agtcccgttg attcattgca
      601 gtctccaatc gcgatcgcgc cgcaatctcc ggcacatgcg ttgcttcacg tcacggtgcg
      661 aagtcgacgc gagcagggat tcaagaagcg tcgtgcgacg tcgagtcacc gccgccatcg
      721 actcgaacgt aattcttaca tgtcccggca ggcaagagcg gcgccgatgt cagtcgaacg
      781 cttgcgcccg ccaccgaatg taatggaggt cgtcatgtcg gtttcgctca tcgcactcat
      841 catcgtcatc ctgcttctgg tcggcgcgct ccccagttgg ggctacagct cgggatgggg
      901 ctactggcca agtgggggac ttgggctcgt gctgctgatt ttgatcatcc tgatggtcat
      961 gggccgcatc tagcggcgtc ggggcgccgc tcgcggcgcc gccatcacct tcttgcgatt
     1021 ggcttcggtc accgggccga tggacgcgcg tgcgcgagcg ggttccgcgc gttggtaatt
     1081 ccgtttgaca ctcaaaatgg ggcagcgtag gttccggcat ccgattgcaa cagggcgggt
     1141 gcgggaatgg cgaaggaggt gttgaagcaa gcgtcgagga cgccagaatc gggcgcaacc
     1201 gacacgcgag agcgcgtgga acgcatgctg caggatatcg agcagcgacg cgaggccgcg
     1261 gtgcgcgagt acgcggcaaa gctcgatggc tggaccggtg acttcgtcgt tgcgccgtcg
     1321 gcgatcgccg ccgcggaagc gagtctctcc gatgaggtca aggatgacct ccgcttcgct
     1381 caccggcaag tcgatgcctt cgcgcgcaag cagcgcgaga gcctgcagga attcgagacg
     1441 gagctctacc ccggactcgt caccggccag cggctgattc cggacaatgt cgccgggtgc
     1501 tatgtcccga gcggacgcta tgcgcacgtg gcttcggcca tcatgagcat cacgacggcg
     1561 aaggcggcgg gcgtaccgca cgtgatcgcc tgctcgccgt cgtaccgcgg acgcggcatt
     1621 catccggtcg ttctttacgc gatggcgctg gcaggcgccg acatcatcat gacgctcggc
     1681 ggcgtgcagg ccatcgcgac gcttgcgctg ggcctcttca gcgatcgccc ggcggatgtg
     1741 attgccgggc cggggaacaa gtacgtggcc gaagccaagc gcatcctgtt cgggcgcgtg
     1801 ggcatcgatg tgttcgccgg gccgagcgaa atctgcgtca tcgccgacga cagcgccgac
     1861 gctgcaatcg tggcgtccga tctcgtgggc caggccgagc acggcgtcga atcgccggca
     1921 tggctcatca cgacgtcgcg gcgcgtcgca tcggaggtgg cggagcgcat tcccgccgtc
     1981 atcgccgccc tgccgtccga agccgcggcc gcggcgggcg ccgcctggcg cgactacggc
     2041 gagatcgtcc tgacggacac gcgcgaggag gcggcgcagg cgagcgacgc ctacgccgca
     2101 gagcacctcg aagtgcacgc gcaggatctc gactggtggc tcgcacgcct taccaattac
     2161 ggctcgctgt tcctcggcga ggagaccacg gtggcctacg gcgacaagac ctcgggaccg
     2221 aaccatatcc tgcccacgcg gcaggcagcc cgctactcgg ggggcttgag cgtcggcaag
     2281 ttcatcaaga cggtgactta ccagcgcatg acgcgtgatg cgacacgccg catcgcgggc
     2341 gtcaccgcgc gcatttcgcg cctcgaaggc atggaagcgc atgcgcgtac cgccgacgac
     2401 cggctgcgaa aatacttccc cggcgaatcc ttcgttacca tgccgtcggt gaacagcgag
     2461 cgcgactgac gacagcatga aagcactggt ctacaccgga ccgaacacga tgcgctaccg
     2521 cgacgagccc gagcccgcgg ccgcgggcgg cgaggtcgtc gtcgaagtgg aggccgtcgg
     2581 catctgcggc tcggacctgc acgcgttcca tggccgcgat ccacgccgcg ttccgccgct
     2641 ga
//
LOCUS       DASSVX010000194         4892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_90717, whole genome shotgun sequence.
ACCESSION   DASSVX010000194 DASSVX010000000
VERSION     DASSVX010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90717"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..1724
                     /gene="pilB"
                     /locus_tag="VFO53_08125"
     CDS             6..1724
                     /gene="pilB"
                     /locus_tag="VFO53_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HET8876279.1"
                     /translation="MATHAATRNISGLARALAQHGVMSEHEAEALQMQSQSSGVSFVE
                     QVLSGKRLSAHQLAVFASRAFGVPLFDLSGFDFDQLNREYFDTRIAQTRRVLPLHKRG
                     NRLYVATSDPANLQALDEVRFKTNLVVEPVVVEDDKLAQAIAKIVESMGTTLKEMAAL
                     EDIEITLEDGSVQAAAADEDSEVEDAPVVRYIQKVLIDAITAGASDIHFEPYERFYRI
                     RYRLDGVLMEVAQPPLAIKDKVASRIKVISKLDIAEKRVPQDGRMKLVLSKTRAIDFR
                     VSTLPTLFGEKIVMRILDSSGVKLGIEALGYEPDQQQALLFAVGRPYGMILVTGPTGS
                     GKTVSLYTCLSILNQPGVNIATAEDPAEIQLPGVNQVNVNEKAGLTFATALRAFLRQD
                     PDVIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPSTLVRMLNMGVAPFNIASSV
                     NIITAQRLARKLCTHCRRPEDIPPEALLRAGFLDEDLDGSWEPFGPVGCDHCKGTGYK
                     GRIGVYEVMPITDEMKQLIMRNGNSLDIAEQAQREGVRNLRQSGLLKVKSGMTSLEEI
                     EAVTNE"
     gene            1753..2979
                     /locus_tag="VFO53_08130"
     CDS             1753..2979
                     /locus_tag="VFO53_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET8876280.1"
                     /translation="MATAVASRDLKVKEQTFLWEGVDRNNKPMRGELRAVSETVASTN
                     LRRQGIRVLKIKRQSYSGGRSISDKDLTFFTRQLATMLKAGVPLLQSFDIIARGHSNA
                     RFSRLMLDLKNKVETGSSLSQAFRDNPKYFDPLYCNLVQAGETSGMLDAILDRLAMYQ
                     EKILAIKSKIKSALFYPITVIVVAFVVVVVIMTFVIPSFKSVFASFGANLPWLTLQVI
                     GISDFFVAYWWAMLIIVVCTFVGLRIAIRRSEHLRYMIHRMQLKLPVIGSILEKATIA
                     RWTRTLQTMFAAGVPLVESLDAVGGASGNLVYVAATKRIQTEVSTGTSLTNSMAATNL
                     FPTMVLQMCQIGEESGSLDNMLGKVADFYEREVDDAVAALSSLLEPVIIVFLGIVVGG
                     LVVAMYLPIFKLGAVI"
     gene            2976..3818
                     /locus_tag="VFO53_08135"
     CDS             2976..3818
                     /locus_tag="VFO53_08135"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET8876281.1"
                     /translation="MTPEPAVAAAIALIVGLCVGSFLNVVVHRLPKMMEREWRAQCAE
                     LRGEAVPDENPPRYNLAVPRSACPACGHRIGALENIPIVSWLALRGACSACRKPISAR
                     YPLVEALGGLLAAYAIWHFGLTWKGVSACVLLWTLIALTFIDFDTQLLPDNLTLPLLW
                     GGLVANLFGLFVSLQDGVIGAITGYLALWLVYWLFRLIRGKEGMGYGDFKLLGALGAW
                     LGWQMLPLIVLLSSVVGAAIGITLMVFKGRDHNVPLAFGPYLAIAGGIALFFGSAIIK
                     GWYL"
     gene            3806..4441
                     /gene="coaE"
                     /locus_tag="VFO53_08140"
     CDS             3806..4441
                     /gene="coaE"
                     /locus_tag="VFO53_08140"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057720.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET8876282.1"
                     /translation="MVSLSTHPYLVGLTGGIGSGKSEVAAAFAARGVDVLDADEIAHA
                     ISKRGEAGHLAVVESLGREFLRADGELDRAALRRRAFDDATFRRDLEHLLHPLIAARL
                     EDAIGDFRGPYGLLVVPLLLERGGLRKRVSRVLVVDCPEQEQIRRVQLRSGLPADEVR
                     RIMATQLPRGERLAEADDVLDNSGPRSAIEPQVARLDRHYRALSAKARETR"
     gene            4510..>4892
                     /gene="zapD"
                     /locus_tag="VFO53_08145"
     CDS             4510..>4892
                     /gene="zapD"
                     /locus_tag="VFO53_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HET8876283.1"
                     /translation="MIRYEHPLNERVRTLMRLEDLFARAIFFATREDAPDHHAALLSL
                     FEITDVAARADLKADLVQELERQKQVLGPLRSNPQIDQQALAALLTEIEQVNASLLAQ
                     AGKVGMHLRENEWLMAIKQRAAIPGG"
BASE COUNT          823 a         1645 c         1593 g          831 t
ORIGIN      
        1 ctgacatggc aacccacgcc gccacccgaa acatcagcgg ccttgcgcgc gcgcttgcgc
       61 agcacggggt catgtccgag cacgaggccg aggcgctgca gatgcagtcg caatcgtccg
      121 gcgtgtcgtt cgtcgagcag gtgctgtcgg gcaagcggct gtcggcgcac cagctcgcgg
      181 tgttcgcgtc gcgcgcgttc ggcgtgccgc tgttcgacct ttccggcttc gatttcgacc
      241 agctgaaccg cgagtacttc gacacccgca ttgcccagac gcgtcgggtt ctgccgctgc
      301 acaagcgcgg caaccgcctc tacgtcgcca cgtccgaccc cgccaacctg caggcgctcg
      361 acgaagtccg gttcaagacg aacctcgtcg tcgagcccgt cgtcgtcgag gacgacaagc
      421 tcgcgcaggc gatcgccaag atcgtcgagt cgatgggcac gacgctgaag gagatggcgg
      481 cgctcgagga catcgagatc acgctcgagg acggcagcgt gcaggccgcc gcggccgacg
      541 aggacagcga ggtcgaggac gcaccggtcg tccgctacat ccagaaggtg ctcatcgacg
      601 cgatcacggc gggcgcctcg gacatccact tcgagccgta cgagcgcttc taccgcatcc
      661 gctatcgtct cgacggcgtg ctgatggaag tggcgcagcc gccgcttgcg atcaaggaca
      721 aggtcgcgtc gcggatcaag gtgatcagca agctcgacat cgccgaaaag cgcgtgccgc
      781 aggacgggcg catgaagctc gtgctgtcga agacccgcgc gatcgacttt cgcgtctcga
      841 cgctgccgac gctcttcggc gagaagatcg tgatgcgtat cctcgattcg tccggcgtga
      901 agctcggcat cgaggcgctc ggctacgagc ccgaccagca gcaggcgctg ctgttcgccg
      961 ttggccgtcc gtacggcatg atcctcgtca ccggaccgac cggcagcggc aagaccgtgt
     1021 cgctctatac gtgcctgtcg atcctgaacc agccgggcgt gaacatcgcc accgccgagg
     1081 atccggccga aatccagctg cccggcgtca accaggtcaa cgtcaacgaa aaggcgggcc
     1141 tcacgttcgc aaccgcgttg cgcgcgttcc tgcgccagga tcccgacgtg atcatggtcg
     1201 gcgaaatccg cgatctcgag acggccgaca tctcgatcaa ggcggcgcag acgggccacc
     1261 tcgtgctgtc gacgctgcac acgaacgacg cgccgtcgac gctggtgcgg atgctgaaca
     1321 tgggcgtcgc gccattcaac atcgcgtcgt ccgtcaacat catcaccgcg cagcgccttg
     1381 cgcggaagct ctgcacgcac tgccggcgtc ccgaggacat cccgcccgag gcgctgcttc
     1441 gcgccggatt cctcgacgaa gacctcgacg gaagctggga accgttcggc ccggtcggct
     1501 gcgaccattg caagggtacc ggctacaagg ggcgcatcgg cgtgtacgaa gtgatgccga
     1561 tcaccgacga gatgaaacag ctcatcatgc gcaacggcaa ctcgctcgac atcgccgagc
     1621 aggcgcagcg cgagggtgtg cgcaacctgc gccaatcggg actgctcaag gtcaagagcg
     1681 gcatgacgtc gctcgaggaa atcgaggccg tcaccaacga gtaatcgcgg gcacgcgaac
     1741 gaaggaaacg tcatggccac cgcagtagcg agcagggatc tcaaggtcaa ggaacagacg
     1801 ttcctgtggg aaggcgtcga tcgcaacaac aagccgatgc gcggcgaact gcgcgccgtg
     1861 tccgagacgg tcgcgtcgac gaacctgcgc cgccagggca tccgcgtgct caagatcaag
     1921 cgccagagct acagcggcgg ccgctcgatc agcgacaagg acctcacgtt cttcacccgc
     1981 cagctcgcga cgatgctgaa ggccggcgtg ccgctgctgc agtcgttcga catcattgcc
     2041 cgcgggcaca gcaacgcgcg cttctcgcgg ctgatgctcg acctcaagaa caaggtcgaa
     2101 acgggctcgt cgctgtcgca ggcgtttcgc gacaacccga aatacttcga tccgctctac
     2161 tgcaatctcg tgcaggccgg cgaaacctcg gggatgctcg atgccattct cgaccggctc
     2221 gcgatgtacc aggaaaagat cctcgcgatc aaaagcaaga tcaagtcggc gctcttctac
     2281 ccgatcaccg tcatcgtcgt cgcgttcgtc gtcgtcgtcg tgatcatgac cttcgtgatc
     2341 ccctcgttca aaagcgtgtt cgcgagcttc ggcgcgaacc tgccgtggct cacgctgcag
     2401 gtgatcggca tctccgactt cttcgtcgcc tactggtggg cgatgctgat catcgtcgtc
     2461 tgcacgttcg tcgggctgcg cattgcaatc aggcgctcgg aacaccttcg ctacatgatt
     2521 caccgcatgc agctcaagct tcccgtcatc ggcagcatcc tcgaaaaggc gacgatcgcg
     2581 cgctggacgc gcacgctgca gacgatgttc gcggccggcg tgccgctggt cgaatcactc
     2641 gacgcggtcg gcggcgcatc gggcaatctc gtctatgtcg ctgcgacgaa gcgcatccag
     2701 accgaagtga gcaccggcac gagcctcacg aactcgatgg cggcgacgaa cctgttcccg
     2761 acgatggtgc tgcagatgtg ccagatcggc gaggagtcgg gctcgctcga caacatgctc
     2821 ggcaaggtcg ccgacttcta cgagcgcgag gtcgacgacg ccgttgcggc gctgtcgagc
     2881 ctgctcgagc cggtgatcat cgtgttcctc ggcatcgtcg tcggcggcct cgtcgtcgcg
     2941 atgtacctgc cgatcttcaa gctcggcgcc gtgatttgac gccggagccc gcagtcgccg
     3001 cggcgatcgc gctgatcgtc gggctttgcg tcggcagctt cctcaacgtc gtcgtccatc
     3061 gcctgccgaa gatgatggag cgtgaatggc gcgcgcagtg cgcggagctg cgcggcgagg
     3121 ccgttcccga cgaaaatccg ccgcgctaca acctcgccgt gccgcgctcc gcgtgtcccg
     3181 cgtgcggcca ccggatcggc gcgctcgaga acatcccgat cgtgtcgtgg ctcgccttgc
     3241 gcggcgcctg ctcggcgtgc cgcaagccga tctccgcgcg ctatccgctc gtcgaggcgc
     3301 tcggcggatt gctcgcggcg tacgcgatct ggcacttcgg actgacgtgg aagggcgttt
     3361 cggcgtgcgt gctgctgtgg acgctgatcg cgctgacgtt catcgacttc gacacgcagc
     3421 tgcttcccga caatctgacg ctgccgcttt tgtggggcgg tctcgtcgcc aacctgttcg
     3481 ggctgtttgt ctcgctgcag gatggcgtga tcggtgcgat caccggctat ctcgccctgt
     3541 ggctcgtcta ctggctgttc cggctcatcc gcggcaagga agggatgggc tacggcgact
     3601 tcaagctgct cggcgcgctc ggtgcgtggc tcggctggca gatgctgccg ctgatcgtgc
     3661 tgctgtcgtc ggtggtcggt gccgcaatcg gcatcacgct gatggtgttc aagggacgcg
     3721 atcacaacgt gccgctcgca ttcggcccgt acctcgccat cgcgggcggc atcgcgctct
     3781 ttttcggatc ggcgatcatc aaggggtggt atctctgagc acccacccct acctcgtcgg
     3841 cctgaccggc ggcatcggca gcggcaagtc ggaagtggcg gcggcattcg ccgcgcgcgg
     3901 cgtcgacgtg ctcgacgccg acgagatcgc gcatgcgatc agcaagcgcg gtgaggcggg
     3961 gcacctggcc gtcgtcgaat cgctcggccg cgagttcctg cgcgccgacg gtgagctcga
     4021 ccgcgccgcg ctgcgccgcc gcgcgttcga cgatgcgacg ttccgccgcg acctcgagca
     4081 cctgctgcat ccgctgatcg ccgcgcgcct cgaggatgcg atcggcgatt ttcgcggccc
     4141 ttacggcctt ctcgtcgtgc cgctgctgct cgagcgcggc ggcctgcgca agcgcgtttc
     4201 gcgcgtgctc gtcgtcgatt gtcccgagca ggagcagatc cgccgcgtgc agcttaggag
     4261 cggcctgcct gccgacgagg tgcgcaggat catggcgacg caactgccgc gcggcgagcg
     4321 gctcgccgag gccgacgacg tgctggacaa ttccggcccg cgcagcgcca tcgagccgca
     4381 ggtcgcccgc ctcgatcggc actaccgcgc cctctccgcc aaggcgcgcg agacgcgctg
     4441 aatcgatctt tcgcgcggcg gcgcgccgcg tttggggcag aatgacgccc tgatgcgaaa
     4501 gccgcgctcg tgattcgcta cgaacaccct ttgaacgaac gggtccgcac gctgatgcgg
     4561 ctcgaagatc ttttcgcgcg ggcgattttc ttcgcgacgc gcgaggacgc accggaccac
     4621 cacgcggcgc tgctatcgct gttcgagatc accgacgtgg cggcgcgcgc cgacctcaag
     4681 gccgacctcg tgcaggagct cgagcgccag aagcaggtgc tgggaccgct gcgcagcaac
     4741 ccgcagatcg accagcaggc gctcgcggcg ctgctgaccg agatcgagca ggtgaacgca
     4801 agcctgctcg cccaggcggg caaggtgggc atgcatctgc gcgagaacga gtggctgatg
     4861 gcgatcaagc agcgcgccgc cattcccggc gg
//
LOCUS       DASSVX010000195        22136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_857837, whole genome shotgun sequence.
ACCESSION   DASSVX010000195 DASSVX010000000
VERSION     DASSVX010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 22136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22136
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857837"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..572
                     /gene="pcaC"
                     /locus_tag="VFO53_08150"
     CDS             183..572
                     /gene="pcaC"
                     /locus_tag="VFO53_08150"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HET8876284.1"
                     /translation="MDEGERYDNGLTVRRAVLGDAHVDRTLARRNDFNEEFQALITRY
                     AWGEIWTRPGLPRHTRSLLTLGLMVALNRPDEFRLHLRAAFNNGVTREEVREVLMHCA
                     IYAGVPAANSAFHWAEEVFAQMDAPAA"
     gene            complement(611..1870)
                     /locus_tag="VFO53_08155"
     CDS             complement(611..1870)
                     /locus_tag="VFO53_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HET8876285.1"
                     /translation="MQASKATGTMINAERLWTSLMDLARVGGTPKGGVRRITLTDADR
                     QGRDLFVRWARDTGLTVRTDEVGNIFARRPGSDPAALPVVIGSHLDTQPSGGKFDGAY
                     GVMAGLEVTRTLNDHGIATRAPIEVVSWTNEEGSRFVPVMMGSGVFAGVFRAEQVLAA
                     KDSDGISAGDALKGIGYAGDGPAHAIDAYFEAHIEQGPVLEDTRTTIGVVQGALGQRW
                     FDVRFSGQDAHAGPTPMDLRHDALIAASHLVLEVNRIARTYPDYARGTVGHMQVKPNS
                     RNVVPGEVQMSVDLRNADDATLRAMADDLTTTARRLAADCRVEAETREVVYFPPCRFA
                     RDLVDSVRESAQALGLGHRDIVSGAAHDAVYLARVAPTAMIFVPCEGGISHNELESAR
                     PEDLAAGCNVLLRAILERAGAREPDAR"
     gene            complement(2103..3599)
                     /locus_tag="VFO53_08160"
     CDS             complement(2103..3599)
                     /locus_tag="VFO53_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HET8876286.1"
                     /translation="MPDPLPIAKHGDLELDLLPALSNRHGLITGATGTGKTVTLQVIA
                     ERMSAIGVPVFMADVKGDLAGMSQPGTMSPKLKARLDTIGAPAPSFAAMPVVFWDVFG
                     EQGHPVRATVSDLGPLLLSRILGLNETQQGVLTLVFKLADDNGLLLLDMKDLRALLQY
                     AGDNAASLTTSYGNVSAASIGAIQRGLLALEQQGGAKFVGEPMLNVDDLLQTDAERGI
                     VNILAAEKLMQSPKLYATMLLWLLSELYERLPEVGDRDKPKLVFFFDEAHLLFTDAPK
                     ALLDKIEQVVRLVRSKGVGVFFVTQNPLDVPDIVLGQLGNRVQHALRAFTPRDEKAVK
                     SAASTMRANPAIDTEKAITELGVGEALISFLDEKGRPNMVERAFVLPPASRIGAITPD
                     ERKAVMDRSPVKGVYEKTVDRESAYEKLATRAAASAQAPTIGTAGTGGGIVDSITGSL
                     GGLMKGSGRKDSLVEAMAKSAMRSVGSSIGRAIVRGVMGSLTGGGRSR"
     gene            3805..4611
                     /locus_tag="VFO53_08165"
     CDS             3805..4611
                     /locus_tag="VFO53_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8876287.1"
                     /translation="MPKIRAGAITMNYDRQGSGEPLILIPHLAADHACYAFQVADYAK
                     HFTCISVDLRGSGESDKPAGRYSTEMFADDVASFMQAAGIDSAHVSGLSLGAATGMWL
                     AAKHPGRVRSLSLHSGWPASDDYIRTVVSGWQVMARALNSATEMVIEAIFPWCLTPEL
                     YAQRPAYVRSLADFLRGRPAQPVDAFVQQSDAVIAHDVEAQLPRIAAPTQITFGRYDA
                     ITSTRFADRLRNGIGSAELHVFPDCSHAPIYENAAAFNEVTLRFLRSCAG"
     gene            4764..5210
                     /gene="rimP"
                     /locus_tag="VFO53_08170"
     CDS             4764..5210
                     /gene="rimP"
                     /locus_tag="VFO53_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET8876288.1"
                     /translation="MSVGELLGQTVPALGYELVDWDMSARTRQIRVFIDKPDGVDVED
                     CARVSNHLTRLFTVENVDFERLEVSSPGLDRPLKRLADYDRFAGQEAQLTLSAPVDGA
                     RRIKGILRGTEGDDVLVETATGVKPFPFGTITQARLVPAIDWRKGK"
     gene            5207..6715
                     /gene="nusA"
                     /locus_tag="VFO53_08175"
     CDS             5207..6715
                     /gene="nusA"
                     /locus_tag="VFO53_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET8876289.1"
                     /translation="MNRELLLLVDALSREKNVPKEIVFTALEAALASATKKKYADNID
                     ARVAIDRETGDYETFRRWTVVPDEEHEEPAHQIAITDAAQRDPALALGDVVEEPLEPV
                     EFGRIGAQAAKQVILQKIRDAEREQILNDFLERDDNLLSGTVKRIERGNVIVESGRIE
                     GLIPRDQLIPKEMLRVGDRMRAYVMKIDRTAKGPQLILSRVAPEFIMRLFELEVPEIE
                     EGLIEIRAAARDPGIRAKIAVKSNDPRLDPQGTCIGMRGSRVTAVTNELAGERVDIVL
                     WSDDPAKLVINALAPAEVQSIVVDEERHAMDVVVDEENLAIAIGRSGQNVRLASEMTG
                     WELNLMSVEESQQKQEAETARLRALFIDKLDVDEEVADILIGEGFTSLEEVAYVPITE
                     MLEIEAFDEDTVNELRSRARNALLTDAIAREESVENVEEALLSLEGMDIDIASKLAAN
                     GITTRDALADLAGDELVELTGMDPSKAQELIMKARAHWFDTPAEASQTSATA"
     gene            6750..9347
                     /gene="infB"
                     /locus_tag="VFO53_08180"
     CDS             6750..9347
                     /gene="infB"
                     /locus_tag="VFO53_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET8876290.1"
                     /translation="MAQTTIEQFATDLKMPAVALLEQLSAAGVAAKKQGDMLSEQDKT
                     RLLDYLRKQHGAAGDGKKRITLTRKSTSEIKAADSQGKARTIQVEVRKKRVLVRREEE
                     AAAAAAAPVVEEAPAVTAAPMLSADEIASREAEERKAQELIARQQEDLQKKQQEAVRR
                     KTKKEREAEEAAARAAAEAKAAEEAKAAAAAVAAVAKPAAKPEGTLHRPAAKPGEKRE
                     KTAKKAPGQVFQEEAARRRALRLRGDTSGGAATGGWRGPKGGRHGREDETGDAAGASA
                     SAGPLVRDIFVPETITVADLAHKMSVKAAEVIKSLMKLGSMVTINQVIDQETAMIVVE
                     EMGHRAKAAKLDDPDALLEETEQAHEAEALPRPPVVTVMGHVDHGKTSLLDTIRRTRV
                     ASGEAGGITQHIGAYHVETRKGTITFLDTPGHEAFTAMRARGAKVTDIVVLVVAADDG
                     VMPQTKEAIAHAKAGNVPIVVAMNKIDKPDANPDRVKQELVAESVLPEEYGGDVQFMP
                     VSAKTGQGIDELLDAVLLQAEVLELKAPKDAPAKGIVIESRLDKGRGPVATVLVHSGT
                     LKRGDVVLAGAVFGRVRAMTDENGKPVNEAGPAIPVEIQGLSDVPLAGEEVLALGDER
                     KAREIALFRQGKFRDVKLAKQHAAKLENMFEQMGESGVKTLTLIVKADVQGSYEALQQ
                     ALTKLSTAEVKVNIVHAAVGGITESDINLALASKAVVIGFNVRADGQARKLAESSGVQ
                     IRYYNIIYEAVDDVKAALSGMLTPEQKENRLGLVEVREVYRISKVGTVAGCYVLDGVV
                     RRGAKVRIVRDSVVVHDGELDSLKRFKDDVREVKAGFECGLSIRNFNDIQQKDQLEVY
                     EIVEVSRTL"
     gene            9352..9723
                     /gene="rbfA"
                     /locus_tag="VFO53_08185"
     CDS             9352..9723
                     /gene="rbfA"
                     /locus_tag="VFO53_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET8876291.1"
                     /translation="MKRPSQRAQRVGDQIQRELADLLKNEVKDPRVGPVTITGVDVSG
                     DLSHATVRFTHLAGKETAKPAATALARTAGFLRSELARRLNLYSVPQLAFVYDDSIES
                     GIRMSQLIDDAIAADKLHPQD"
     gene            9720..10649
                     /gene="truB"
                     /locus_tag="VFO53_08190"
     CDS             9720..10649
                     /gene="truB"
                     /locus_tag="VFO53_08190"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET8876292.1"
                     /translation="MTAMSAPRRTPRRHVDGVLLLDKPRGITSNAALLRVKRLYRAEK
                     AGHTGTLDPLASGLLPVCFGAATRFAGFLLDAPKRYVATIRFGVTTTTLDAEGGVVAS
                     REVAFGQDALAAALTRFTGPQMQTPPAHSALKFEGRPYYDYARQGVDIARAPRPVVIH
                     ALRLLQWQAPDAVVEVECSKGTYVRVLAADVGEALGCGAHLAALRRTASGGFALRDAV
                     TFDALDAMGEAALDACLKPVSVLVGHLPALIVAGDAARRFRHGGAVPAGECDDGLVAA
                     YEADSLLGIAFVSHGIANPRRTIASPGAATEPA"
     gene            10724..10993
                     /gene="rpsO"
                     /locus_tag="VFO53_08195"
     CDS             10724..10993
                     /gene="rpsO"
                     /locus_tag="VFO53_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET8876293.1"
                     /translation="MAVTVTEKAKVIAENQRANGDTGSPEVQVALLTARINGLNDHFK
                     ANVKDHHSRRGLLRMVSRRRKLLDYLKSRNTDSYRSLIEKLGLRK"
     gene            11170..13302
                     /gene="pnp"
                     /locus_tag="VFO53_08200"
     CDS             11170..13302
                     /gene="pnp"
                     /locus_tag="VFO53_08200"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET8876294.1"
                     /translation="MPNPIKKSIAYGSHTLTLETGEIARQANGAVVVSLGDTVVLVTC
                     VAAKQPKPGQDFFPLTVDYQEKTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPLRPL
                     FPEGLYNDVQIVATVLSVDPEIDPDIPAMIGASAAVSLSGIPFDGPIGAARVGYANGQ
                     YMLNPTKTDLATSQLNLVVAGTEAAVLMVESEANELPEEVMLGAVVFGHQQQQAVIEL
                     IDQLVEEGGKPLWDWQPPTKDDALIARVAASGESELRDAYRLRSKQARSQRIREIASK
                     VEADCVDADASPDARRHVHNILFDLEARIVREQILSGEARIDGRDTRTVRPISIRTGV
                     LPRTHGSALFTRGETQAIVVATLGTARDEQIVDALMGEYRDRFMFHYNMPPFATGETG
                     RVGSPKRREIGHGRLAKRAILAVLPTAEEFAYSLRVVSEITESNGSSSMASVCGASLA
                     LMDAGVPTKAHVAGIAMGLIKEGNRFAVLTDILGDEDHLGDMDFKVAGTDRGITALQM
                     DIKIQGITKEIMAVALAQAREGRLHILGLMHEAVPHARTEVSEHAPRMMTFKINPEKI
                     RDVIGKGGSVIRALTEETGTTIDIQDDGSITIASVNAEACNVARKRIEAITAEVEVGK
                     IYEGPVLRLLDFGAIVQLLPGKDGLLHISQIAKERVNQVSDYLKEGQIVRVKVLEADE
                     KGRVRLSMKAAAEDDAKQHQPAESTPAA"
     gene            complement(13378..13821)
                     /locus_tag="VFO53_08205"
     CDS             complement(13378..13821)
                     /locus_tag="VFO53_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET8876295.1"
                     /translation="MQLTRFTDYALRVLLFVGREQGRRCTIAEIAHYYRISPEHLRKV
                     VHRLAKIGYLASSRGKGGGLVLGRDPLHIRIGDVITAMEEQLCVVDCHALDCVLLPHC
                     SLKVALDRASGAFVAAMNEVTLANLLGERRMMRQMTSVDTIARAT"
     gene            complement(13894..14529)
                     /locus_tag="VFO53_08210"
     CDS             complement(13894..14529)
                     /locus_tag="VFO53_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876296.1"
                     /translation="MDHGAALSVAGNHERSPRRRWSAAILLAILLVPFVRDVMEASML
                     SQMLVQIPLLAVAGWLARSCLTIAARARIAAWNASGITGFIVASATSACWMLPRALDG
                     AVADPLIDAAKYLSVPLLLGLPLSLSWPQAGFVARGVVLAEAIASLFRLGWIYRVSPV
                     RLCSAYPLADQQQLGLCMLAIGGMLVAAIAWRLLFGDPRHAGSRTYRGMIT"
     gene            complement(14536..15174)
                     /locus_tag="VFO53_08215"
     CDS             complement(14536..15174)
                     /locus_tag="VFO53_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET8876297.1"
                     /translation="MSSLFRTLLASAFAVALGTVALASATDGFRAFTSETARRAAVAR
                     SPVAVPQAVLETDRGTRLTLADLRGTWLVVDFVYTRCATYCTAQGASFAQVQRLLARE
                     IGDARVQLLSISFDPAHDPPSQLAAYLRRSGDRGSGWIAARPVAADALERLLHGFGVT
                     VIADGLGGYVHNEGVHIVDPQGRIVAIVDGGDPVAIADALSSRRVARARPPA"
     gene            complement(15143..16585)
                     /locus_tag="VFO53_08220"
     CDS             complement(15143..16585)
                     /locus_tag="VFO53_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET8876298.1"
                     /translation="MRSDERFVLGLYLVTVLAVFILMMLLGLAMRMAQGTWLDVAPDL
                     FYRIMTAHGAGMIGTTTLAASAVMWFFLRKYVPLRLPFFVANYVLFLLGAVAILSSVF
                     VGGYAGAWTFLYPLPVHSMGLWSVNAAVLFMLGYLLIGVGFLLFYLDAMRALIATYGS
                     LGRALGLQWLFGGRIDTSHPVAVVASTMVAITNTLGILAGAVVLVLCIVNAYLPSIPL
                     DALLVKNLIYFFGHTFINAAIYMAVIAVYELLPRYTGRPWPLSRATLWGWATSSVMVL
                     IVYPHHLMMDFVMPHWMLTMGQIVSYSAGLPVFVVTAYGALTNVYRSGIRWTMPAALV
                     VLSMFGWAAGIVPAIVDGTIRVNQVMHNTQWVPGHFHFYLLAGVLPMVFALMFHLAGE
                     RSLRSRLDGIGFFAYTAGTLVFVMAFLAAGQASVARRFAVHLPEWTPYDKAGSIAAIV
                     IVSAMLVFAVRIVSALMRRPVVVVVPYAAR"
     gene            complement(16684..17232)
                     /locus_tag="VFO53_08225"
     CDS             complement(16684..17232)
                     /locus_tag="VFO53_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase"
                     /protein_id="HET8876299.1"
                     /translation="MSVQGEAWLVTLVGLALVVLAFVHVVVQASKPPPPPPADGRHLA
                     YRLRGWLFAALVIFGAGVTYATLRTFPIPVQSADAAGAQIVQVTAQQWRWDIKPDSVT
                     AGTPVEFRVASADVNHGFAIYGPDERIVTQTQAMPGFTNRLVYTFATPGTYRILCLEY
                     CGLAHHGMQAEVHVTAAPGARS"
     gene            complement(17341..18345)
                     /gene="cydB"
                     /locus_tag="VFO53_08230"
     CDS             complement(17341..18345)
                     /gene="cydB"
                     /locus_tag="VFO53_08230"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003185655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET8876300.1"
                     /translation="MHLDLVPIWTLILGFGVLMYVLLDGFDLGIGILAPMAHDDDTRT
                     LMMNTVAPIWDGNETWLILGGMGLLAAFPLAFAIIIPALYFPILLMLLGLIFRGVAFE
                     FRLKKTSHRHWWDASFFGGSAVATFFQGVVLGMYVEGFTVTGRVFSGTSFDWITPFPL
                     ATGVGLLFGYTLLGATWLVMKTEGKLQQDARRWALVALYGVLAFIAMVSIWTPLLHQR
                     IAERWFSVPNLYYFALVPIVTALIAFATWRALRRDADYVPFIGAMGLFAMCYLGLGIS
                     LFPYIVPYALTLWDAAAVPSTQAFLLIGTLFLLPIIFMYTGWSYYVFRGKVTADVGYH
                     "
     gene            complement(18357..19742)
                     /locus_tag="VFO53_08235"
     CDS             complement(18357..19742)
                     /locus_tag="VFO53_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET8876301.1"
                     /translation="MELDPVFLSRLQFAFVIAFHILLPAFTVGLASFIVLLEGLHLVK
                     RDPLYLRLSVFWTRIFAVSFGMGVVSGIVMPFQFGTNWSRYTDATGNILGPLFAYEGL
                     IAFFLESGFLGILLFGRKRVPPVAHFFAAVMVAAGTLFSSFWILAANSWMQTPVGYDV
                     VDGRYVPADWLAVIFNPSFPYRLVHTVTGFFLTTAFAVLGVAAWHLKHDRHVTEARTV
                     LGMTVIFVAIFVPIQIFMGDLHGINTLHHQPVKLAAMEGLWDTQSHAPAVLFAIPDEA
                     AERNRDEISIPALASYYLGHSADAVIKGLKEAPPADRPPVAPVFFAFRVMVGIGVLML
                     AIAYWGVWLRWRGRLYTSRAFHNACLAMLPAGFVAVVAGWTVTEVGRQPWVVYGLMRT
                     RDAVSPSLTGTDVLTSIALYILVYLVVFGAGIFYMVRLARAGPPEHVDVREPNLDERP
                     ARPLSGAHADT"
     gene            complement(19761..20567)
                     /locus_tag="VFO53_08240"
     CDS             complement(19761..20567)
                     /locus_tag="VFO53_08240"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HET8876302.1"
                     /translation="MTNEWIRAVVAGVALYPIAFCAGAQQPAPAPQPGYVAPPAYSTP
                     PNLGTQTTLIQVPSPQPVITITASATNEVANDRMLAMLRAETDNADAARAAGEVNARM
                     ARALSQAKSVSGIDARTTGYSSYQISEPNRPLRWRVSQTLALESGDFVALSALVSKLQ
                     GSDGLLLSGLSFTVSPSARHAAEDALTTQAIKSWQQRAQNAARAFGAGDFRTGRVTIQ
                     TNDFGRPQPMFKAGGVSATSVAPVSVEGGTSEVTVTVSGEAILDTVRVPR"
     gene            complement(20575..21042)
                     /gene="nudB"
                     /locus_tag="VFO53_08245"
     CDS             complement(20575..21042)
                     /gene="nudB"
                     /locus_tag="VFO53_08245"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HET8876303.1"
                     /translation="MGSKQPVSVLVIVHTANLHVLLLERTDFPGHWQSVTGSRDQDED
                     LPATARRELLEETGIDADRYAGPVDWHLARQYEIFPQWRHRYAPGTTMNTEHVFSLDV
                     GTPVPVLLSAREHLRHVWLPWREAAERCFSWTNRDAILALPDRVRAAGPRHEG"
     gene            complement(21072..21461)
                     /locus_tag="VFO53_08250"
     CDS             complement(21072..21461)
                     /locus_tag="VFO53_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HET8876304.1"
                     /translation="MKLPDLSNYDEARVLDYHGHADAVEAAVSHAKIRYLDVDLSHAD
                     DKATLFAALAAGLKLPEYFGKNFDALADVLEDRDWLGKRGCAIRLAHATHYRKTHPND
                     WGTLEEILSEASTFWRDRHLPFWVLVS"
     gene            complement(21458..21856)
                     /locus_tag="VFO53_08255"
     CDS             complement(21458..21856)
                     /locus_tag="VFO53_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HET8876305.1"
                     /translation="MTRLTGPRAPVRATLLTIVCVFLAVLTAPAHAREPGPRGDIEAA
                     ALPREARDVLALIHAGGPFRYERDGVTFGNRERSLPPRRRGYYHEYTVATPGVRTRGA
                     RRIVCGGARATPEVCYYSDDHYATFRRIRE"
     gene            complement(21905..>22136)
                     /locus_tag="VFO53_08260"
     CDS             complement(21905..>22136)
                     /locus_tag="VFO53_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET8876306.1"
                     /translation="LKALQAGAPPHGGIAFGLDRIVAMMCKAESIRDVIAFPKTQRGQ
                     DLLVDAPAPATEQQLRDLHLRVRPSDVAKATG"
BASE COUNT         4047 a         7264 c         7448 g         3377 t
ORIGIN      
        1 gggcgacctg tcgacgccgc cgcaggacgg gcgctacatc ggtgatcaga tcgagggtgc
       61 gcgctgcgtc gagctgccag ccacccacct gtcgaatatc caggcggcgc ccgcgttcac
      121 gcaggcactc gtgcaattcc tcaccggccg cggttagcga cctcaccgaa cgggatcgaa
      181 ccatggatga aggcgaacgc tatgacaacg gactgacggt gcgccgagcg gtcctgggcg
      241 atgcgcacgt cgatcgcacg cttgccaggc gcaacgattt caacgaggaa tttcaggcgc
      301 tcatcacgcg ctatgcgtgg ggcgagatct ggacgcggcc gggtttgccg cgccatacgc
      361 gcagcctgct gacgctcggc ctcatggtcg ccctgaaccg tcccgacgag tttcggctgc
      421 accttcgcgc cgccttcaac aacggcgtga cccgcgagga agtgcgcgag gtgctgatgc
      481 attgcgcgat ctacgccggc gtcccggccg ccaattccgc attccactgg gcggaggagg
      541 tgttcgcgca gatggatgcg ccagctgcgt aggttgcccc gttgccgcgg cgcacgcgcc
      601 gtccggcgca ttaccgggca tccggctcgc gcgcgcctgc gcgctccaga atcgctcgca
      661 gcagcacgtt gcaccccgcg gccaggtctt cgggccgcgc gctttcgagc tcgttgtggc
      721 tgatgccgcc ctcgcagggg acgaatatca tcgcagttgg cgcaacgcgg gcgaggtaca
      781 ccgcgtcgtg cgccgcgccg cttacgatgt cgcgatggcc aagaccaagc gcctgtgccg
      841 attcgcgaac cgaatcgacg aggtcgcgcg cgaagcggca cggtggaaag tacaccacct
      901 cgcgagtttc ggcttcgacg cggcagtcgg cagcaagcct gcgcgcggtc gttgtcaggt
      961 cgtccgccat cgcgcgcagc gtggcgtcgt ccgcattgcg aaggtcgacg ctcatctgca
     1021 cttcgccggg caccacgttg cgcgaattgg gcttgacctg catgtggccg acggtgcctc
     1081 gcgcgtagtc gggatacgtt cgcgcgatgc ggttgacttc gagcaccagg tggctcgccg
     1141 cgatcagcgc gtcgtgacgc aggtccatcg gcgtcggccc tgcgtgcgca tcctgtccgc
     1201 tgaagcggac gtcgaaccag cgctggccca acgcgccctg caccacgccg atcgtggttc
     1261 gcgtgtcctc gagcaccggt ccctgctcga tgtgcgcctc gaaatacgcg tcgattgcgt
     1321 gcgctggccc atcgccggcg tagccgatgc ctttcaatgc gtcgccggcg ctgatgccgt
     1381 cactatcctt cgctgcgagc acctgttcgg cgcggaacac gccggcgaac acgcccgacc
     1441 ccatcatgac cggcacgaac cgcgagcctt cctcgttcgt ccaggaaacg acttcgatag
     1501 gcgcgcgcgt tgcaatcccg tgatcgttca acgtgcgcgt cacctcgagg cccgccatca
     1561 cgccatacgc accgtcgaat ttgccgcccg acggttgcgt atcgagatgg ctgccgatga
     1621 ccaccggcag agcggccggg tcgcttcccg gacgccgggc gaaaatgttg cccacctcgt
     1681 ccgtgcgcac cgtgagcccc gtgtcgcgcg cccagcgcac gaagaggtcg cgaccctggc
     1741 gatcggcgtc tgtcagcgta atgcgccgca cgccgccctt gggcgtgccg ccgacgcgcg
     1801 caagatccat cagcgacgtc cagagccgct cggcgttgat catcgttccc gtcgctttcg
     1861 atgcttgcat tcggcgtcct tttcttgcgt cgacacaagg cgtctgctgc gatgcggcgc
     1921 gtaccggcct agtgacctgt aacgggggaa tcggcagtgc ctggcttgct ctccggcgcg
     1981 atcgctgcgt tggcgctgct tgcaaggggg cctacccttg ctgcgcgtcg ccgccttgcg
     2041 ctcgcgccgg atagcggcgc catgtcactc gcgttcacta ccgattcccc cgttacaggc
     2101 cactagcggc tgcgtccgcc gccggtcagc gagcccatca cgccgcgcac gatcgcccgt
     2161 ccgatcgacg agccgacgct gcgcatcgcg ctcttcgcca tcgcctcgac caggctgtcc
     2221 ttgcggccgc tgcctttcat gagcccgccg agcgagcccg tgatcgagtc gacgatgccg
     2281 ccgccggtcc cggccgtgcc gatcgtcggc gcctgggccg acgccgcggc gcgcgtggcg
     2341 agcttctcgt acgccgactc gcggtcgacc gtcttctcgt acacgccttt caccggcgag
     2401 cggtccatca ccgccttgcg ctcgtcgggt gtgatcgcgc cgatgcgcga tgcgggcggc
     2461 agcacgaacg cgcgctccac catgttcggc cggcccttct cgtcgagaaa cgagatcagc
     2521 gcctccccga caccgagctc ggtgatcgcc ttctcggtgt cgatggccgg attggcgcgc
     2581 atcgtcgacg ccgccgactt cacggccttc tcgtcgcgcg gcgtgaatgc gcgaagcgcg
     2641 tgctgcacgc ggttgcctag ctgcccgagc acgatgtcgg gcacgtcgag cggattctgc
     2701 gtcacgaaga agacgccgac gcccttcgag cgaacgagcc gcacgacctg ctcgatcttg
     2761 tcgagcagcg ccttcggcgc gtcggtgaag agcagatgcg cctcgtcgaa gaagaacacg
     2821 agcttcggct tgtcgcgatc gccgacttcc ggcagccgct cgtagagctc ggagagcagc
     2881 cacaaaagca tcgtcgcgta cagcttcggc gactgcatca gcttctccgc ggcgaggata
     2941 ttgacgatcc cgcgctcggc gtcggtctgc agcagatcgt cgacgttcaa catgggttcg
     3001 ccaacgaatt tggcgccgcc ctgctgctcg agggcgagca agccccgctg gatcgcgccg
     3061 atcgacgccg ccgagacgtt gccgtacgag gtggtaaggc ttgccgcgtt gtctccggcg
     3121 tattgcagca gcgcgcgcag gtccttcatg tcgagcagca gcaggccgtt gtcgtcggcg
     3181 agcttgaaga cgagcgtcag gacgccttgc tgcgtctcgt tcagccccaa tatccgactg
     3241 agcagaagcg gtccgagatc gctcacggtg gcgcgcaccg ggtgtccctg ctcgccgaag
     3301 acgtcccaga acaccacggg catcgccgcg aacgacggtg ccggcgcgcc gatcgtatcg
     3361 agccgcgcct tgagcttggg ggacatcgtg cccggctggc tcatgccggc gagatcgccc
     3421 ttgacgtcgg ccatgaacac cggcacgccg atggcgctca tgcgctccgc gatcacctgc
     3481 agcgtcacgg tcttgccggt gccggttgca ccggtgatca gtccgtgccg gttggacagt
     3541 gcgggcagca ggtcgagttc gagatcgccg tgcttggcga tgggcagggg atcgggcatg
     3601 gcgggagcct gttcagacga tgtccgctta cgcggacggg tggcatgggg cgccgatcga
     3661 ggcgccggcg tttacaacgt cgaaagcgca gctcgagttt agcgcgccgg cgaaggcggc
     3721 ggcgtatgct gacgcctttg tcgcttcagg tcaccggagg tccgggcgtt gcccccccgc
     3781 cggcgacgtt ccccgggagc cgccatgccg aaaatccgcg ccggcgcgat cacgatgaac
     3841 tacgatcggc aggggagcgg cgagccgctg atcctcattc cgcatctcgc cgccgaccac
     3901 gcctgttacg cgttccaggt tgccgattat gcaaagcact tcacctgcat ctcggtcgac
     3961 ttgcgtggaa gtggcgaatc cgacaagcca gccggccgat actcgacgga aatgttcgcc
     4021 gacgatgttg cctcgttcat gcaggcggca ggcatcgaca gcgcgcacgt cagcggactt
     4081 tcgctcgggg cggcgaccgg catgtggctc gcggcgaagc atcccgggcg cgtccgctcg
     4141 ctgtccctgc acagcggctg gccggcaagc gacgactaca tccggaccgt cgtcagcggc
     4201 tggcaggtca tggcacgcgc gttgaacagt gcgacggaga tggtgatcga ggcaatcttc
     4261 ccgtggtgcc tgacgcccga gctctatgcg caaaggccgg cgtacgtccg gtcgctggcc
     4321 gatttcctgc gcggtcgtcc tgcgcagccc gtagacgcgt tcgtccagca gtccgacgcg
     4381 gtgatcgcgc acgacgtcga ggcgcaattg ccgaggatcg ctgcgccgac gcagatcacg
     4441 ttcgggcgct acgatgcgat cacgtcaacg cgcttcgccg atcggctcag gaatggcatc
     4501 ggaagcgccg agcttcacgt ctttccggat tgctcgcacg cccccatata cgagaatgcg
     4561 gcggcgttca acgaggtgac gctccggttc ctgaggagct gcgccggatg aagcgctccc
     4621 cacggcgcgg gcattctgcc tggacgcgtg gcgtctgcca cccccggatg tcaatcgcgc
     4681 cgattttctg ctagaatacg gcccgataag taatcagaaa tgggctgggc gcagcccatt
     4741 tttttttggt tttcgaaggt tgaatgagcg ttggtgaact gctcgggcaa accgtaccgg
     4801 cgttggggta cgaactcgtc gactgggaca tgtcggcgcg gacgcgccag atccgcgtct
     4861 tcatcgacaa gccggacggc gtcgatgtcg aggactgtgc ccgcgtatcg aaccacctga
     4921 cgcggctctt caccgtcgaa aacgttgatt tcgagcggct cgaagtgtcg tcgccgggcc
     4981 tcgaccgacc gctcaagcgg cttgccgact acgaccggtt cgcgggccag gaagcccagt
     5041 tgacattgag cgcgccggtc gatggcgcgc gacgcatcaa aggaatcctg cgcggcacgg
     5101 aaggggacga cgtcctcgtc gaaaccgcga ccggcgtgaa gccgttcccg ttcggaacga
     5161 tcacgcaggc gcggctcgtg cccgccatcg attggagaaa aggaaaatga accgcgaact
     5221 actgttgctc gtggacgcgc tctcgcgcga gaagaacgtg ccgaaggaaa tcgtgttcac
     5281 ggcgctcgaa gccgcgctcg cgtcggccac caagaagaag tatgccgaca acatcgacgc
     5341 gcgcgtcgcc atcgaccgcg agaccggcga ttacgagacg ttccgccgct ggacggtcgt
     5401 gcccgacgag gagcacgagg agcccgcgca ccagatcgcg atcaccgacg ccgcgcagcg
     5461 cgacccggcg ctcgcgcttg gtgacgtcgt cgaggagccg ctcgagccgg tcgaattcgg
     5521 tcgcatcggc gcgcaggcgg caaagcaggt catcctgcag aaaatccgcg acgccgagcg
     5581 cgagcagatc ctgaacgact tcctcgagcg cgacgacaac ctgctctcgg gaacggtgaa
     5641 gcgcatcgag cgcggcaacg tcatcgtcga atcgggccgc atcgagggcc tgatcccgcg
     5701 cgaccagctc attccgaagg aaatgctgcg cgtcggcgac cgcatgcgcg cctacgtgat
     5761 gaagatcgac cgcacggcga aagggccgca gctgatcctg tcgcgcgtcg cgcccgagtt
     5821 catcatgcgg ctcttcgagc tcgaggttcc ggagatcgag gaagggctga tcgagattcg
     5881 cgccgccgcg cgcgaccctg gcatccgcgc gaaaatcgcc gtcaagtcca acgatccgcg
     5941 gctcgacccg caaggcacgt gcatcggcat gcgcggctcg cgcgtcaccg cggtgaccaa
     6001 cgagctcgcc ggcgagcgcg tcgatatcgt gctgtggtcc gacgatccgg cgaagctcgt
     6061 gatcaacgcg ctcgcgccgg ctgaagtgca aagcatcgtc gtcgacgagg agcggcatgc
     6121 gatggacgtc gtcgtcgacg aggagaacct cgccatcgcc atcggccgca gcggccagaa
     6181 cgtgcgtctc gcctccgaga tgacgggttg ggagctgaac ctgatgagcg tcgaggaatc
     6241 gcagcagaag caggaagccg agaccgcgcg ccttcgcgcg ctcttcatcg acaagctcga
     6301 cgtcgacgag gaggtggcgg acatcctgat cggcgagggc ttcacgtcgc tcgaggaagt
     6361 cgcctacgtg ccgatcaccg aaatgctcga gatcgaggcg ttcgacgagg acacggtgaa
     6421 cgagcttcgc tcgcgtgcgc gcaatgcgct gctcaccgac gccatcgcac gcgaggaaag
     6481 cgtcgagaac gtcgaggagg cgctgctctc gctcgaaggc atggacatcg acatcgcgag
     6541 caagctcgcg gccaacggca tcaccacgcg tgacgcgctg gccgatctcg cgggcgacga
     6601 actggtcgaa ttgaccggca tggacccgtc gaaggcgcag gaactcatca tgaaggcgcg
     6661 cgcgcactgg ttcgacacac cggcggaagc gtcgcaaacg agtgcgacgg cgtaagggca
     6721 aggcagctta ggcggtacgg gagaagcgca tggcgcagac gacaatcgaa caattcgcga
     6781 cggacctcaa gatgccggcc gtcgcgttgc tggaacagct ttccgcggct ggcgtcgcgg
     6841 ccaagaagca gggcgacatg ctctccgagc aggacaagac gcgcctgctc gattacctgc
     6901 gcaagcagca cggtgcggcc ggcgacggca agaagcgcat cacgctcacc cgcaagtcga
     6961 cgtccgaaat caaggcggcc gactcgcagg gcaaggcgcg cacgattcag gtcgaggtgc
     7021 gcaagaagcg cgtgctcgtc cgccgcgagg aagaggcggc ggcggccgcg gcagcgcctg
     7081 tcgtcgagga agcgcctgcc gtcaccgcag cgcccatgct gagcgccgat gaaatcgcat
     7141 cgcgcgaggc ggaagaacgc aaggcgcagg agctcattgc ccgccagcag gaagacctgc
     7201 agaagaagca gcaggaagcg gtgcggcgca agacgaagaa ggaacgcgag gccgaggaag
     7261 cggcagcgcg cgccgctgcc gaagcgaagg ccgccgagga ggcaaaggcg gcagccgccg
     7321 ctgtcgccgc cgtggcgaag cccgcggcca agcccgaagg cacgctgcat cgcccggccg
     7381 cgaagcccgg cgagaagcgc gaaaagacgg cgaagaaggc gcccggacag gtgttccagg
     7441 aagaggccgc gcgtcgccgt gcgctgcgcc tgcgcggcga tacgagtggc ggcgccgcca
     7501 cgggtggctg gcggggtccc aagggcggcc ggcatggccg cgaggacgaa accggcgacg
     7561 cggcgggcgc atcggcgtcg gcgggcccgc tcgtgcgcga catcttcgtg cccgagacga
     7621 tcaccgtggc cgatctcgca cacaagatgt cggtgaaggc tgcggaagtc atcaagtcgc
     7681 tgatgaagct cggatcgatg gtgacgatca accaggtgat cgaccaggaa acggcgatga
     7741 tcgtggtcga ggaaatgggc caccgcgcga aggcggcgaa gctcgacgat cccgatgcgc
     7801 tcctcgagga gaccgagcag gcgcacgagg ccgaggcgct gccgcgtccg ccggtcgtca
     7861 ccgtgatggg gcacgtcgac cacggcaaga cgtcgctgct cgacacgatc cgccgcacgc
     7921 gcgtcgcaag cggcgaggcg ggcggaatca cgcagcatat cggcgcctat cacgtcgaaa
     7981 cgcggaaggg caccatcacg ttcctcgaca caccgggcca cgaggccttt acggccatgc
     8041 gcgcgcgggg tgcgaaggtc accgacatcg tcgtgctggt ggttgcggca gacgacggcg
     8101 tgatgccgca gacgaaggaa gcgatcgcgc acgcgaaggc cggcaacgtt ccgatcgtcg
     8161 tcgcgatgaa caagatcgac aagcccgacg ccaatcccga ccgcgtcaag caggagctcg
     8221 ttgccgaaag cgtgctgccc gaggagtacg gcggcgacgt gcagttcatg ccggtgtcgg
     8281 cgaagaccgg gcagggtatc gacgagttgc tcgacgccgt cctgctgcag gccgaggtgc
     8341 tggagttgaa agcgccgaag gatgcgccgg cgaagggcat cgtcatcgaa tcgcggctcg
     8401 acaagggccg cggaccggtc gctaccgtgc tcgtgcattc cggcacgttg aagcgcggcg
     8461 acgtcgtgct cgccggcgcg gtgttcggac gcgtgcgcgc gatgaccgac gagaacggca
     8521 agccggtcaa cgaggccggt cccgcgatcc cggtggaaat acagggcctg tccgacgtgc
     8581 cgcttgccgg cgaggaagtg ctggcgctcg gcgacgagcg caaggcgcgc gagatcgcgc
     8641 tgttccgcca gggcaagttc cgcgacgtca agctcgcgaa gcagcatgcg gcgaagctgg
     8701 agaacatgtt cgagcaaatg ggcgagtcgg gcgtcaagac gctgacgctg atcgtcaagg
     8761 ccgacgtgca gggcagctac gaggcgctgc agcaggcgct cacgaagctg tccaccgccg
     8821 aggtgaaggt caacatcgtg catgcggcgg tgggcggcat caccgaatcg gacatcaacc
     8881 tggcgctcgc gtcgaaggcg gtggtgatcg gcttcaacgt gcgcgccgac gggcaggcgc
     8941 gcaagctcgc ggagtcgagc ggcgtgcaga tccgctacta caacatcatc tacgaagcgg
     9001 tcgacgacgt gaaggctgcg ctgtccggca tgctgacgcc cgagcagaag gagaaccggc
     9061 tcggactcgt cgaggtgcgc gaggtctatc gcatctcgaa ggtcggaacc gtggccggat
     9121 gctacgtgct cgacggcgtc gtgcgacgcg gcgcgaaggt ccggatcgtg cgcgacagcg
     9181 tcgtcgtcca cgatggcgag ctcgattcgc tgaagcgctt caaggacgat gtgcgcgaag
     9241 tgaaggccgg cttcgaatgc gggttgtcga tcaggaactt caacgacatc cagcagaagg
     9301 accagctcga ggtctacgag atcgtggagg tctcgcgcac gctgtgaacc gatgaaacgt
     9361 ccctcgcagc gcgcacagcg cgtcggcgac cagatccagc gcgagctcgc cgacctcctg
     9421 aagaacgagg tcaaggatcc gcgcgtcggc ccggtgacca tcaccggcgt cgacgtgtcg
     9481 ggcgatcttt cgcacgcgac cgtgcgcttc acgcatcttg ccggcaagga aactgccaag
     9541 cccgcggcta cggcgctcgc ccgcaccgcg ggattccttc gcagcgagct tgcacggcgc
     9601 ctgaacctgt attcggtacc gcagctggcg ttcgtctacg acgattcgat cgaatccggc
     9661 atccggatgt cgcaattgat cgacgacgcg atcgcggccg acaagctgca tccgcaggac
     9721 tgaccgcgat gtccgcacct cgtcgtacac cccgccggca cgtcgacggc gtgctgctgc
     9781 tcgacaagcc gcgaggcatc acgtcgaatg cagcgctgtt gcgtgtcaag cggctgtatc
     9841 gtgccgagaa ggcgggtcac accggcacgc tcgatccgct tgcgtcgggc ctgctgccgg
     9901 tctgcttcgg cgccgccacc cgcttcgcgg gattcctgct cgacgcgccg aagcgctatg
     9961 tcgccaccat ccgcttcggg gtgacgacga ctacgctgga tgcggaaggc ggcgttgtcg
    10021 cgtcgcgcga ggtggcgttc gggcaggatg cgctagctgc tgcgctcacg cgtttcaccg
    10081 gcccgcagat gcagacgccg cccgcgcatt cggcgctcaa gttcgagggc cggccgtatt
    10141 acgactacgc gcgccagggt gtcgacatcg cgcgcgcgcc gcgtccggtc gtcattcatg
    10201 cgttgcggct cctgcaatgg caggcacccg acgccgtcgt cgaggtcgaa tgcagcaagg
    10261 gcacgtacgt gcgcgttctc gctgccgatg tcggcgaggc gttgggctgc ggcgcgcatc
    10321 ttgcagcact tcggcgcacg gcgagcggcg gcttcgcgct tcgcgacgcg gtgacgttcg
    10381 acgcactgga cgcgatgggc gaggcagccc tcgatgcgtg cctgaagccc gtcagcgtgc
    10441 tcgtcgggca tctgccggcg ctgatcgttg caggcgacgc cgcgcggcgg tttcgtcacg
    10501 gtggtgcggt gcccgcaggc gagtgcgacg acggactggt tgccgcgtac gaggccgatt
    10561 cgctgctcgg catcgccttc gtgtcgcacg ggatcgccaa tccgcgccgg acgatcgcat
    10621 cgcccggcgc ggccactgaa cctgcttgaa aaacaagcat tttccgcgct ataatcgccc
    10681 tcttccccac gagctgaaaa acgagagaac gaaggacatt gacatggccg ttacggtaac
    10741 cgaaaaggcg aaggtcatcg ccgaaaacca gcgcgcgaac ggggacaccg gttcgcccga
    10801 agtccaggtc gcgctcctca ccgcccgcat caacgggctc aacgaccact tcaaggcaaa
    10861 cgtcaaggat catcattcgc gccgcggcct gttgcggatg gtttcgcgcc ggcgcaagct
    10921 tctcgactac ctgaagtcgc gcaacaccga cagctatcga tcgctgatcg agaagctcgg
    10981 actgcgcaaa tagcgcaatc gcgttgtcgc acgcgccgct ccattcttcc ccgagcacca
    11041 cgacgcattc gatcgtcatc cgtttgcgtc gcggacatcc cggacagcgc gcgctctccg
    11101 gctgaaaccc atcgcaatgg ccgcgtcgat gcacgcggcc attgtcattc ttgaattgcg
    11161 aggaacattg tgccgaaccc catcaagaag tccatcgcct acggcagtca cacactgacg
    11221 ctcgaaaccg gcgaaatcgc ccgccaggcg aacggcgcgg tcgtcgtcag tctcggcgac
    11281 accgtcgtgc tcgtcacctg cgtcgcggca aagcagccga agcccggaca ggatttcttt
    11341 ccgctgaccg tcgactatca ggaaaagacg tacgccgcgg gcaagatccc gggtggcttc
    11401 ttcaagcgcg aagggcgccc gtcggaaaag gaaacgctca cgtcgcggct catcgaccgg
    11461 ccgctgcgcc cgctgttccc cgagggcctc tacaacgacg tgcagatcgt cgccaccgtg
    11521 ctctccgtcg atcccgaaat cgatcccgac attcccgcga tgatcggcgc atcggcggcc
    11581 gtgtcgctgt cgggcattcc gtttgacggt ccgatcggcg cggcgcgcgt cggttatgcg
    11641 aacgggcagt acatgctcaa tccaaccaaa accgatctcg cgacatcgca gttgaacctg
    11701 gtcgttgccg gcaccgaagc cgccgtgctg atggtcgaat ccgaagcgaa cgagctgccc
    11761 gaggaagtga tgctcggcgc ggtcgtgttc ggtcaccagc aacagcaggc ggtgatcgag
    11821 ctcatcgatc agctcgtcga ggagggcggc aagccgctgt gggactggca gccgccgaca
    11881 aaggacgacg cgctgatcgc acgcgtcgcc gcgtcgggcg aatcggaact gcgcgatgcg
    11941 tatcgacttc gttcgaagca ggcgcgctcg cagcgcattc gtgagatcgc ctcgaaggtc
    12001 gaggccgact gtgtcgacgc cgacgcgtcg cccgacgcgc gccgtcacgt gcacaacatc
    12061 ctgttcgatc tcgaagcacg catcgtgcgc gagcagatcc tctcgggcga ggcgcgcatc
    12121 gacgggcgcg acacgcgcac cgtgcgtccg atctccattc gcaccggcgt gctgccgcgc
    12181 acgcacggct cggcgctgtt cacccgcggc gagacgcagg cgatcgtcgt cgcgacgctc
    12241 ggcaccgcgc gcgacgagca gatcgtcgac gcgctgatgg gcgaatatcg cgaccgcttc
    12301 atgttccact acaacatgcc gccgttcgcc accggcgaga ccggccgcgt cggttcgccg
    12361 aagcggcgcg agatcggcca tggccgcctt gcgaagcgcg cgatcctcgc cgtgttgccg
    12421 accgccgagg agttcgccta ttcgctgcgc gtcgtgtccg agatcaccga gtcgaacggc
    12481 agctcgtcga tggcgtcggt gtgcggcgcc agcctcgcgc tgatggacgc aggcgtgccc
    12541 accaaggcgc acgtcgcggg catcgcgatg ggcctcatca aggaaggcaa tcgctttgcc
    12601 gtgctgaccg acatcctcgg cgacgaggat catctgggcg acatggactt caaggtcgcg
    12661 ggcaccgatc gcggcatcac cgcgctgcag atggacatca agatccaggg catcacgaag
    12721 gaaatcatgg cggtggcgct cgcgcaggca cgcgaagggc ggctgcatat cctgggcctg
    12781 atgcacgagg cggtgccgca tgcgcgcacc gaagtctcgg agcacgcgcc gcgcatgatg
    12841 acgttcaaga tcaacccgga aaagatccgc gacgtgatcg gcaagggcgg ctcggtaatc
    12901 cgcgcgctga ctgaggagac gggaacgacg attgacatcc aggacgacgg ctcgatcacc
    12961 atcgcatcgg tcaacgccga agcgtgcaac gtcgcgcgca agcgcatcga ggcgatcacg
    13021 gcggaggtcg aagtcggcaa gatctacgaa ggtccggtgt tgcgcctgct cgacttcggg
    13081 gcgatcgtgc agcttctgcc gggcaaggac gggctgctcc atatctcgca aatcgcgaag
    13141 gagcgggtca accaggtttc cgattacctg aaggaagggc agatcgtgcg cgtcaaggtg
    13201 ctcgaggccg acgagaaggg ccgcgtgcgc ctgtcgatga aggccgccgc cgaagacgac
    13261 gcgaagcagc accagccggc ggagtcgacg ccggcggctt gagtttcgtc gaagagaagc
    13321 acacgagggc gcccgcgggc gccctcttcg catccggcgc ccgcgccacc gacgccatca
    13381 cgttgcgcgg gcgatcgtgt cgacgctcgt catttgtctc atcattcgcc gctcgccgag
    13441 gaggttagcg agcgtcacct cgttcatcgc ggcgacgaag gcgccgctcg cgcggtcgag
    13501 cgcgaccttg agtgagcaat gcggcagcag cacgcagtcg agcgcatggc aatcgaccac
    13561 gcacaactgc tcctccatcg cggtaatcac gtcgccgatg cgaatgtgca aagggtcgcg
    13621 tcccagcacg agcccaccgc ccttgccgcg tgacgatgcg aggtagccga tcttcgccaa
    13681 ccggtgcacg accttgcgca ggtgctcggg cgagatgcgg tagtagtgcg caatctcggc
    13741 gatggtgcag cggcggccct gctccctgcc gacgaacagg agcacgcgca gcgcatagtc
    13801 ggtaaagcgg gtgagctgca tgatcgcggg tattcgcgtg cgcccgcggc cgtagcggca
    13861 ggcattggcg acgaatctct gacgtggtgg gattcacgtg atcataccgc ggtatgtgcg
    13921 tgatccagcg tggcgcggat cgccgaagag cagcctccac gcaatggccg cgacgagcat
    13981 gccgccgatt gcgagcatgc aaagtcccag ttgctgctgg tcggcaagcg ggtaggcgct
    14041 gcacagccga accggcgaca cccgatagat ccagccgagg cgaaacaggc tcgcgatcgc
    14101 ctcggcgaga accacgccgc gcgcgacgaa gcccgcttgc ggccagctga gcgagagcgg
    14161 caggccgagg aggagcggca ccgaaaggta ttttgccgcg tcgatcaagg gatcggcgac
    14221 ggcgccgtcg agcgcgcgcg gaagcatcca gcatgcggac gtcgccgatg ccacgatgaa
    14281 gccggtgatc ccgcttgcgt tccacgccgc gatccgcgcg cgtgcagcga tggtcagaca
    14341 ggagcgagcg agccagcccg caacggccag gagcgggatc tgcacgagca tctgcgagag
    14401 catgctcgcc tccatgacat cgcggacgaa cggcacgagc aggatcgcaa gcaggatcgc
    14461 cgcgctccag cgccgccgcg gcgaccgctc gtggttgccc gcaaccgaca gggcagcgcc
    14521 gtgatccatc gggcgtcacg ccggtgggcg cgcccgcgcc acgcgtcgcg acgacagcgc
    14581 atccgcgatg gcgacgggat ccccgccgtc gacgatcgcg acgatccgtc cctgcggatc
    14641 gacgatatgc acgccctcgt tgtgcacgta gccgccgagt ccatcggcga tgacggtgac
    14701 gccgaacccg tgaagcagtc gctcgagcgc gtcggccgcc acgggccgtg cagcgatcca
    14761 gccgctgccg cgatcgcccg agcgacgcag gtatgccgca agctgcgacg gcgggtcgtg
    14821 cgcggggtcg aagctgatgc tgagcagttg cacgcgggcg tcgccgatct cgcgtgcgag
    14881 caggcgctgc acctgcgcga agctcgcgcc ctgggcggtg cagtacgtcg cgcagcgcgt
    14941 gtagacgaag tcgacgacga gccatgttcc gcgcaggtcg gcgagcgtta agcgcgtgcc
    15001 acggtcggtc tcgagcaccg cctgcggcac cgctaccggg gatcgtgcaa ctgcggcgcg
    15061 gcgcgcggtt tcgctggtga acgcgcgaaa gccgtcggtc gcgctggcga gcgcgacggt
    15121 gccgagcgcg accgcgaacg cgctagcgag cagcgtacgg aacaacgacg acaacgggcc
    15181 gccgcatgag tgcggagacg atgcgcacgg caaagacgag cattgccgac acgatgacga
    15241 tcgcggcgat cgaccccgcc ttgtcgtacg gcgtccattc gggcagatgc acggcgaagc
    15301 gtcgcgcgac gctcgcctgt ccagcggcga ggaacgccat cacgaaaacc agcgtaccgg
    15361 cggtataggc gaagaagccg atgccatcga gccgcgatcg cagcgagcgc tcgccggcga
    15421 gatggaacat cagcgcgaat accatcggca gcacgccggc cagcaggtag aagtggaagt
    15481 gcccgggaac ccattgcgtg ttgtgcatca cctgattcac gcgaatggtg ccgtcgacga
    15541 tcgcgggcac gatacccgcg gcccagccga acatggacag cacgacgagt gccgcgggca
    15601 tcgtccagcg gatgcccgag cggtagacgt tggtcagcgc gccgtacgcc gtcacgacga
    15661 acaccggcag ccccgccgaa tacgacacga tctggcccat cgtcagcatc cagtgcggca
    15721 tgacgaagtc catcatcagg tgatgcggat agacgatcag caccatgacg ctgctcgttg
    15781 cccagcccca cagcgtcgcg cgcgacagcg gccagggccg gccggtatag cggggaagca
    15841 gttcgtacac ggcgatcacc gccatgtaga tcgccgcgtt gatgaatgtg tggccgaaga
    15901 agtagatcag attcttgacg agcagagcat cgagcgggat cgacgggagg tatgcgttga
    15961 cgatgcacag gacgaggacg accgcgccgg cgagaatgcc gagcgtattg gtgatcgcaa
    16021 ccatcgtcga tgcaacgact gcaaccggat gcgacgtgtc gatcctgccg ccgaacagcc
    16081 actgcagtcc gagcgcgcgg ccgaggctgc cgtaggtcgc tatcagcgca cgcatcgcgt
    16141 cgagatagaa gagcaggaac ccgacaccga tcagcaggta gccgagcatg aacagcacgg
    16201 ccgcattgac gctccacagt cccatcgaat gcacgggcag cggatagagg aacgtccatg
    16261 caccggcgta tccgccgacg aacacggacg acaggattgc caccgcgccg aggaggaaca
    16321 gcacgtagtt cgcgacgaag aacggcagcc ggagcggcac gtacttgcgc aggaagaacc
    16381 acatcaccgc cgacgcggcg agcgtcgtcg tgccgatcat gcccgcgccg tgggccgtca
    16441 tgatgcggta gaagaggtcg ggtgcgacgt cgagccatgt cccctgggcc atgcgcatcg
    16501 caaggcccag cagcatcatc aggatgaaca ccgccagcac cgtcacgagg tagagcccga
    16561 gcacgaagcg ctcgtcggac ctcagcgctt cgctcgccgg aaagcgctcg cttgcgtggt
    16621 cccgcagcac tcgcggcttc gccgcggcgc tggaattcgc ggcttgggac gacccggccg
    16681 cgctcatgat cgtgctcccg gcgcagccgt cacatgcacc tcggcctgca tgccgtgatg
    16741 cgccagcccg cagtattcga ggcagagaat ccgatacgtg cccggcgtcg cgaacgtgta
    16801 gacgaggcgg ttcgtgaatc ccggcatggc ctgcgtttgc gtgacgatgc gctcgtcggg
    16861 accatagatc gcgaagccgt gattgacgtc ggcgctcgcc acgcgaaact cgacgggcgt
    16921 acctgccgtg acgctatccg gcttgatgtc ccagcgccat tgttgcgcgg tcacctgcac
    16981 gatctgcgcg cccgcggcgt cggccgactg gacaggaatg gggaaggtcc gcagggtcgc
    17041 gtaggtgacg ccggcgccga agatcacgag cgccgcgaaa agccagccgc gcagtctata
    17101 ggcgagatgc ctgccgtccg ccggtggcgg cggcggcggc ttcgacgcct ggacgacgac
    17161 atgaacgaat gccaggacca cgagagcaag cccgaccagc gtgacgagcc atgcttcgcc
    17221 ttgaacgctc atggcgacct ccgggggagg ggcgcacaaa gatgtgcgcg aaaaacatta
    17281 tatgacccga aggccggtcg ccgtcaatcc ccccgccttg ttgccgctcg ggtgccccga
    17341 tcagtgatat ccgacgtccg ccgtgacctt cccgcggaac acgtaatacg accagccggt
    17401 gtacatgaag atgatcggca gcaggaacag cgtgccgatc agcaggaacg cctgcgtcga
    17461 cgggaccgcg gccgcgtccc agagcgtgag ggcgtacggc acgatgtacg ggaagaggct
    17521 gatgccgagg ccgagatagc acatcgcgaa gagtcccatc gcgccgatga acgggacgta
    17581 atcggcgtcg cgccgcaacg cccgccaggt ggcgaaggca atcagcgcgg tcacgatcgg
    17641 cacgagggcg aaataataga ggttcggcac gctgaaccag cgctcggcga tccgttggtg
    17701 cagcagcggc gtccatatgc tcaccatggc gatgaacgcg agcacgccgt acagcgcgac
    17761 gagcgcccag cgccgcgcat cctgctgcag cttgccttcg gtcttcatca cgagccatgt
    17821 cgcgccgagc agcgtgtagc cgaacaacag cccgacacct gtcgccagcg ggaacggcgt
    17881 gatccagtcg aagctcgtgc ccgagaacac acggcccgtg accgtgaacc cttcgacgta
    17941 catgccgagc accacgccct ggaagaacgt cgcgaccgcc gacccgccga agaacgacgc
    18001 gtcccaccag tggcgatggc tcgtcttctt caacctgaac tcgaacgcga cgccgcggaa
    18061 gatcagtccg agcagcatca gcaggatcgg aaaatagagc gccggaatga tgatcgcgaa
    18121 cgcgagcggg aatgcggcga ggagtcccat gccgccgagg atcagccacg tctcgttgcc
    18181 gtcccagatc ggcgccaccg tgttcatcat cagcgtccgc gtgtcgtcgt cgtgcgccat
    18241 cggcgcgagg atgccgatgc cgaggtcgaa cccgtcgagc agcacgtaca tcaagacgcc
    18301 gaagccaaga atgagcgtcc atatcggtac gaggtcgaga tgcatcgcgg ctcctgtcac
    18361 gtgtcggcgt gggcgccgga gagcggccgt gcggggcgct cgtcgaggtt gggttcgcgc
    18421 acgtcgacat gctcgggcgg gcccgcgcga gcgaggcgca ccatgtagaa aattccggcg
    18481 ccgaacacga caagatagac gaggatgtat agcgcgatcg acgtgagcac gtcagtaccg
    18541 gtcagcgatg gcgatacggc gtcgcgcgtg cgcatcaagc catacacgac ccacggctgg
    18601 cgtcccacct cggttaccgt ccaaccggcg acgacggcaa cgaaacctgc gggaagcatc
    18661 gcgagacacg cattgtgaaa cgcgcgcgac gtgtagagcc gcccgcgcca gcgcagccac
    18721 acaccccagt acgcgatggc cagcatcagc acgccgatgc ccaccatgac gcgaaacgca
    18781 aagaacaccg gcgcgaccgg tggacggtcc gccggtggtg cttccttcag ccccttgatg
    18841 accgcatcag ccgaatggcc gaggtaatag ctcgcaagcg ccggaatgga gatctcgtca
    18901 cgattgcgct ccgccgcttc atccggaatg gcgaacagca cggcgggcgc atgcgactgc
    18961 gtatcccaca ggccttccat ggctgcgagc ttcaccggct gatgatgcag cgtgttgatg
    19021 ccatggaggt cgcccatgaa gatctggatc ggcacgaaga tcgcgacgaa gatcaccgtc
    19081 atgcccaaaa cggtacgggc ttccgtcaca tgccggtcgt gcttcagatg ccatgcggcc
    19141 acaccgagca cggcgaacgc cgtcgtcagg aagaaaccgg tgacggtatg cacgagacga
    19201 tacggaaacg acggattgaa gatcacggcc agccagtctg ccggcacgta gcgtccgtcg
    19261 acgacgtcgt agccgacggg cgtttgcatc cagctattgg cggcgagaat ccagaacgac
    19321 gagaacagcg tgccggcggc gaccatgacc gcggcaaaga aatgcgccac tggcggcacg
    19381 cgcttgcggc cgaacagcag gatgccgagg aaaccggact cgaggaagaa ggcgatgaga
    19441 ccttcgtacg caaacagcgg gccgagaata ttgccggtgg cgtcggtgta gcggctccag
    19501 ttggtgccga actggaacgg catcacgatg ccggacacga cacccatgcc gaacgacacg
    19561 gcgaagatgc gcgtccagaa caccgacagc cgcagataca acggatcgcg cttgacgagg
    19621 tgcaggccct cgagcaatac gatgaacgag gcgagcccga cggtgaatgc cggcagcagg
    19681 atgtgaaacg cgatcacgaa cgcgaactgc aggcgcgaca ggaagaccgg gtcgagttcc
    19741 attgttcttg ccctccggcc ctacctgggc acgcgcactg tatcgagaat cgcttctccg
    19801 ctcaccgtga cggtgacttc gctcgtgccg ccttcgacgc tcaccggtgc gacgctggtg
    19861 gcggatacgc cgcccgcctt gaacatcggc tgcggacggc cgaaatcgtt cgtctggatc
    19921 gtgacacgtc cggtgcgaaa gtcgcccgcg ccgaatgcgc gcgcggcgtt ctgcgcccgc
    19981 tgctgccagc ttttgatcgc ctgcgtcgtc agcgcgtcct cggcggcatg gcgcgcggac
    20041 gggctcacgg tgaagctgag ccccgacagc agcaatccgt cgctgccctg cagcttcgaa
    20101 acgagcgcgg acagcgccac gaagtcgccg ctttccagtg ccagcgtctg cgagacgcgc
    20161 cagcgcagcg gccggttcgg ctcggagatc tggtaggagc tgtagcccgt cgtgcgcgca
    20221 tcgatgccgc tgacggactt cgcctgcgac agcgcgcggg ccatgcgcgc attcacctcg
    20281 cccgcggcgc gcgcggcgtc ggcattgtcg gtttcggcgc gcagcatggc gagcatccgg
    20341 tcgttggcga cttcgttcgt tgccgacgcg gtgatcgtga tgacgggctg cggcgaaggc
    20401 acctggatca gcgtcgtttg cgtgccgaga ttcggcggcg tggaatacgc cggtggcgcg
    20461 acgtagcccg gttgcggcgc gggcgccggc tgttgcgcgc cggcgcaaaa cgcaatcgga
    20521 tagagcgcga cgcccgccac gaccgcacga atccattcat tcgtcattgc gtcatcatcc
    20581 ctcgtgtcgc gggccggcgg cgcgcacgcg atcgggcaac gcgagaatcg cgtcgcgatt
    20641 ggtccacgaa aagcagcgct cggccgcctc gcgccagggc aaccatacat ggcgcagatg
    20701 ctcacgtgca gacagcagca ccgggacggg cgttccgacg tcgagcgaga acacgtgctc
    20761 ggtattcatc gtcgtgcccg gcgcgtaccg gtgccgccat tgcggaaaga tttcgtactg
    20821 ccgggcgaga tgccaatcga ccggtcccgc gtagcgatct gcatcgatgc cggtctcctc
    20881 gagcaattcg cgccgcgcgg ttgccggaag gtcctcgtcc tggtcgcgac tgccggtgac
    20941 ggattgccag tgcccgggaa agtcggtgcg ctcgagcaac agcacgtgca gattcgccgt
    21001 gtgcacgatc accagaaccg ataccggctg cttggagccg atctccgcgt ccgctggcat
    21061 cgtcgccgat ctcagctcac cagcacccag aacggcaggt gccggtcgcg ccagaacgtc
    21121 gacgcttcgg acaggatttc ctcgagcgtg ccccagtcgt tcgggtgcgt cttccggtag
    21181 tgcgtcgcgt gcgccaggcg gatcgcgcag ccgcgcttgc cgagccagtc gcggtcctcg
    21241 agcacgtcgg ccagcgcatc gaagttcttg ccgaagtatt ccggcagctt gagtcccgcc
    21301 gcgagcgcgg cgaaaagcgt cgccttgtcg tcggcgtgcg agaggtcgac gtcgagatat
    21361 cggatcttcg cgtggctcac cgcggcctcg accgcgtcgg cgtgcccgtg gtagtcgagc
    21421 acgcgcgcct cgtcgtaatt ggacagatcc ggcagcttca ttcgcggatc cttcggaacg
    21481 tcgcgtagtg gtcgtcgctg taatagcaca cttcgggcgt cgcgcgtgcg ccgccgcaaa
    21541 cgatccggcg cgcgccccgc gtgcgcacgc cgggtgtcgc aacggtgtac tcgtgatagt
    21601 agccgcgcct gcgcggcggg agcgaacgct cacgattgcc gaacgtcacg ccgtcacgct
    21661 cgtatcggaa ggggccgccg gcatggatca gcgcgagcac gtcgcgcgct tcgcgcggca
    21721 gcgcggcggc ttcgatgtcg ccgcgcggac ctggctcgcg cgcatgcgcc ggcgcggtca
    21781 ggacggcgag gaacacgcag acgatcgtga ggagcgtcgc gcgaaccggc gcgcgcgggc
    21841 ccgtgagccg agtcatgcga cgcagtttag cgcgtccgtc gccgacgcgc gcaggtgccg
    21901 ccgatcagcc ggtggccttc gccacgtcgg aaggccggac ccggaggtgc aggtcgcgca
    21961 attgctgctc ggtggcgggc gcaggcgcgt cgacgagcag atcctggcca cgttgcgtct
    22021 tcgggaacgc aatcacgtcg cggatcgact ccgccttgca catcatggcg acgatgcggt
    22081 cgaggccgaa tgcgatgccg ccatgcggtg gcgcgcccgc ctgcagtgcc ttcagc
//
LOCUS       DASSVX010000196         6739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1557955, whole genome shotgun sequence.
ACCESSION   DASSVX010000196 DASSVX010000000
VERSION     DASSVX010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557955"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1127)
                     /locus_tag="VFO53_08265"
     CDS             complement(165..1127)
                     /locus_tag="VFO53_08265"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET8876307.1"
                     /translation="MIVTRLTQAFGLRHPIVLAPMGGAAGGRLAAAVSNAGGLGLVGG
                     GYGEREWMTRELALAGAGTQAQWGVGLITWSIGPGILELALAHRPGAVMLSFGDPRPH
                     ARAIKAAGSALICQVQGVDEARLAHEAGADFIVAQGTEAGGHGGSRATLPLVPAIVDL
                     VAPIPVIAAGGIVDGRGVAAALMLGAQGALMGTRFCASTEALGRESSKQRLVAARGGE
                     TARTRVFDIVRDYGWPAPYTGRALRNDFMTRWNGREDALAATLDVERVAYRRAAADDD
                     YATALVWAGEGVDLITSVEPAAALVARIGADAEARLRAGAGLLR"
     gene            1364..2047
                     /locus_tag="VFO53_08270"
     CDS             1364..2047
                     /locus_tag="VFO53_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004935171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET8876308.1"
                     /translation="MTRQIADRFEREVALPARGASLTGDLTVPAGAKGVVLFAHGSGS
                     SRRSPRNVWVAARLQRAGLATLLFDLLTGAEEQRDVYTREHRFDIPLLAKRLVLATDW
                     IDTQPELQTLSVGYFGASTGAAAALVAAHERKDRVAAVVSRGGRPDLAGADVLASVAA
                     ATLLIVGGNDDAVLDLNRGALASMTCEKRLDIVPGATHLFEERGALEQVATLASQWLV
                     EFLVVQAAR"
     gene            2060..2716
                     /locus_tag="VFO53_08275"
     CDS             2060..2716
                     /locus_tag="VFO53_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HET8876309.1"
                     /translation="MRFANRMQAGDVLAQKLLRYRDDPSVVVLALPRGGVPVAFQVAH
                     ALHAPLDVFIVRKLGLPGHEEFAMGAIASGGVMVMNPDLANFHIPETAIEKVVARERT
                     ELDRRERLYRDDRAPLAIEDRTVILVDDGLATGSTMLAAATAVRKRQPRSIVVAVPVA
                     ATETCQAFRHVVDEVICAVTPEPFFAVGAWYDDFGQTSDAEVHALLDAAWRGAERGAA
                     "
     gene            2806..3537
                     /locus_tag="VFO53_08280"
     CDS             2806..3537
                     /locus_tag="VFO53_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET8876310.1"
                     /translation="MNTARRSLLGLFAAAIVSIGGLAHAQETLRVATDATFPPFEYTE
                     NGKRVGFDVELIEAIAKVLNRKVEWTDIDFKGLIPGLLAKRFDVAASAIYITDERRKV
                     VDFTDPYYPGGLVIMTKKGDQTIKGPDDLKGKKVSVQVGTKSVNFLKETFPATERVEV
                     EKNQEMFQLVEVGRADAAVTGKPAAKLYARARGTLQVVDKQLTVEEYGYALRKDEPQL
                     LQQFNDALKKLKADGTYDKLVAKYL"
     gene            3560..4228
                     /locus_tag="VFO53_08285"
     CDS             3560..4228
                     /locus_tag="VFO53_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET8876311.1"
                     /translation="MDFDFSPVWAGWRELLHGTLITIEVTAGAIVLGCLIGLLIGLGR
                     LDARRRVVYAVCTAYVSFIRGTPLLVQLFIWFFGLPHFGINLPAFVCGVLGLGIYSGA
                     YVSEVVRGAIRSIDRGQMEAARSLGMSYPMAMRQIVIPQAIVRMIPPLGNEFVALIKN
                     SSLVSLLTITDVMHEGQKIISVSYRSLEVYLAIALVYFVLTNLTGLALRTVERRLGVG
                     GLVQ"
     gene            4225..5073
                     /locus_tag="VFO53_08290"
     CDS             4225..5073
                     /locus_tag="VFO53_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET8876312.1"
                     /translation="MRPEASPQPIVSARGITKSFGPLAVLKGVDCDVMPSQVSVVIGP
                     SGSGKSTLLRCCNGLEVADGGTIDICGQRLVDHGTMLRDPALNALRMQVGMVFQSFNL
                     FPHLSVLGNLTIGPRKLRGLSIAAAQAEARALLDKVGLRDKIDAYPSTLSGGQKQRVA
                     IARALAMRPQVMLFDEPTSALDPELVGEVLQVMKNLAREGMTMLVVTHEMGFAREVAD
                     VVSVMDHGVVLESGPPAQVFDDPAHPRTRAFLQAVITRGGPRRSVAPAATSDASIVDS
                     LIVDDH"
     gene            complement(5102..5878)
                     /locus_tag="VFO53_08295"
     CDS             complement(5102..5878)
                     /locus_tag="VFO53_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HET8876313.1"
                     /translation="MDELGGGARRRFLGRLAASLGALGVAGCDRLSQSDWFPKLLGAA
                     EPLNYAAAKLVTGRKAMAQEFTPADLSPAFRSNGTAEPDNAQYTALAANHFADYALDV
                     GGLVAKPRRFTLAELRALPSRTQITRHDCVEGWSAIGKWQGAKLSAVLAAVEPSERAR
                     YVVFHCADPMEQDGTSPYYESIDMDDAYHVQTLLAYALNDAPLPVKNGAPIRLRVERQ
                     LGYKHAKYVMRLELVESFAGIGEGKGGYWEDQGYQWYAGV"
     gene            complement(5871..6641)
                     /locus_tag="VFO53_08300"
     CDS             complement(5871..6641)
                     /locus_tag="VFO53_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET8876314.1"
                     /translation="MHWINAIALALMLMSGLQIFNAHPALYVGDSSYSGRPAWLEIGA
                     RDDAQGKPVGYTRLFGHEFITTGVLGLSTDDARMYAQGFPSWLTIPSHQWLAMARSWH
                     FFFAWVFLINGLCFIAYTIASRHLARDLLPTRGDIRGIGTSIVDHLHFRHPAGEAAKR
                     YNVLQKFAYLTVIFVLLPLIVLMGLGMSPRLDTLFGGWVGWFGGRQTVRSIHFIVAWL
                     LVAFVLIHVFEVIVTGFWNNLRSMITGRYRIPQEKQHG"
BASE COUNT         1114 a         2279 c         2249 g         1097 t
ORIGIN      
        1 ccggtacgcc gccgacgacg gcgaatggtt catgcacgga ctgtttgcat gagcggcgac
       61 gggccgcccc gagccgcgaa ttcgccccct gcgggggcag cgcagcggcg aagccgcaag
      121 cgtgggggtt cctgagcggc atcccgaccg catgcgaaac tgcctcagcg caacagaccc
      181 gcgcccgcgc gaagccgcgc ctcggcgtcg gcgccaatgc gcgcgacgag cgctgcggca
      241 ggctcgacgc tcgtgatgag atcgacgccc tcacccgccc atacgagcgc ggtcgcgtag
      301 tcgtcgtcgg cggcggccct gcggtaggcg acgcgctcga cgtcgagcgt ggcggcgagc
      361 gcgtcttcgc ggccgttcca gcgcgtcatg aagtcgttgc gaagcgcgcg cccggtgtac
      421 ggtgccggcc atccgtagtc gcgcacgatg tcgaacacgc gggtgcgcgc cgtttcgccg
      481 ccgcgcgccg cgaccaggcg ttgcttggaa ctctcgcgcc ccagcgcttc cgtgctcgcg
      541 caaaatcgcg tgcccatcag cgcaccctgc gcgccgagca tcagcgccgc ggccacgccg
      601 cgcccgtcga caattccacc ggcggcgatc accggtatcg gcgcgacgag atcgacaatg
      661 gcgggaacga gcggaagcgt cgcccggctt ccgccgtggc cgccggcctc ggtgccctgt
      721 gcgacgatga aatcggcacc cgcctcgtgc gcgagtcgcg cctcgtccac gccctgcacc
      781 tggcagatga gcgcgctgcc cgctgccttg atcgcacgcg catgcggtcg cgggtcgccg
      841 aacgacaaca tgaccgcgcc gggccgatgc gcgagtgcca actcgaggat gccgggaccg
      901 atcgaccacg taatgaggcc gacaccccac tgcgcctgcg tcccggcgcc ggcgagcgca
      961 agttcacgcg tcatccattc gcgctcgcca tagccgcctc cgacgagacc gagtccgccg
     1021 gcgttcgaca cggccgcagc aaggcggcca cccgctgcgc cacccatcgg cgccagcacg
     1081 atcggatgcc gcaacccgaa cgcctgcgtc aatcgcgtaa cgatcattcg aagccctcac
     1141 gtgcgcaaaa ggcgccgctc ttctcacgcg gcagtctagc aatcgggcag tcgagggcca
     1201 acagggcccg cgcgttgtaa ctattacatc gatacgcgca cgacctgccc gctgcgcaca
     1261 ttgcaatcca cgcctcgcgc atgcgttgcc gtgcgcgcgc caacccgcga cgaccggtac
     1321 gtttattgcg cacacccggt gacatcctgc aaggagttgc cagatgacgc ggcagattgc
     1381 cgatcgcttc gagcgcgaag tggcgcttcc cgcgcgcggc gcatcgctca ccggggatct
     1441 caccgtgccc gccggcgcaa aaggcgtcgt attgttcgcg cacggcagcg gcagcagccg
     1501 caggagtcca cgcaacgtct gggtcgcggc acgactgcag cgcgccgggc tggcaacgct
     1561 cctcttcgat cttctgaccg gcgccgaaga gcaacgcgac gtgtacacgc gcgagcaccg
     1621 cttcgacatt ccgctgctcg cgaagcgtct cgtgctcgcc accgactgga tcgatacgca
     1681 gcccgaactg caaacgctgt ccgtcggcta tttcggcgcg agcaccggcg cggctgcagc
     1741 actggttgcg gcgcacgaac gcaaggatcg cgtcgctgcc gtcgtttcgc ggggcgggcg
     1801 gccggatctg gccggcgccg acgtgctcgc cagcgtggcg gcggcgacgc tcctgatcgt
     1861 cggcggcaac gacgatgccg tgctcgatct caatcgcggt gcgctcgcgt cgatgacctg
     1921 cgagaagcgg ctcgacattg tgcccggtgc gacacatctg ttcgaggaac gcggtgcgct
     1981 cgagcaggtg gcaacactcg cgtcgcagtg gctcgtcgag ttcctcgtcg tgcaggcggc
     2041 acgctgaagg aacgaggcca tgcgcttcgc gaatcggatg caggcgggcg acgtgcttgc
     2101 gcagaagctg ctgcgctatc gcgatgaccc gagcgtggtc gtgctcgcgc taccgcgcgg
     2161 cggtgttccc gtggccttcc aggtcgcgca cgcgctgcat gcgccgctcg acgtattcat
     2221 cgtgcgcaag ctcgggctgc cggggcacga ggaattcgca atgggcgcga tcgcgagcgg
     2281 cggcgtcatg gtgatgaatc ccgatcttgc gaactttcac atccccgaga cggcgatcga
     2341 gaaggtcgtc gcgcgcgagc gcaccgagct cgaccggcgc gagcggcttt atcgcgacga
     2401 tcgcgcaccg ttggcgatcg aggaccggac ggtgatcctc gtcgacgacg gtcttgcaac
     2461 gggctcgacg atgctcgccg cggcgaccgc ggtgcgcaag cgccagccgc gatccatcgt
     2521 cgtggcggtg ccggtggccg cgaccgaaac ctgccaggcg tttcgccacg tcgtcgacga
     2581 ggtgatttgc gccgtcacgc cggaaccgtt tttcgcggtc ggcgcgtggt acgacgattt
     2641 cggccagacc agcgacgccg aagtgcatgc actgctcgac gccgcatggc gcggcgcaga
     2701 acgcggcgcg gcctgaagcg gcgcttccgc ggcgctcgcg ggttcgcgag taccatgtgg
     2761 ccctgccggc gcatgccggt ttttggtcca ctccagggga atcagatgaa taccgctcgc
     2821 cgcagcctgc tcgggctgtt cgccgccgcc atagtgtcga tcggcggtct cgcccatgcg
     2881 caggaaacgc tgcgcgtcgc gaccgacgcg acgtttccgc cattcgagta tacggagaac
     2941 ggcaagcgcg tcggcttcga cgtcgaattg atcgaagcga tcgcgaaggt gctgaaccgc
     3001 aaggtcgaat ggaccgacat cgacttcaag ggactcatcc cgggactgct cgccaagcgc
     3061 ttcgacgttg cggcgtcggc gatctacatc accgacgagc gacgcaaggt cgtcgacttc
     3121 accgatccgt attacccggg gggtctcgtc atcatgacga aaaagggcga ccagacgatc
     3181 aagggaccgg acgatctcaa ggggaagaag gtttcggtgc aggtcggcac gaagtcggtc
     3241 aatttcctga aggagacttt tcccgccacc gagcgcgtcg aggtcgagaa gaatcaggag
     3301 atgttccagc tcgtcgaagt gggccgcgcc gacgctgccg tcaccggcaa gcctgcggcg
     3361 aagctgtacg cccgcgcacg cggaaccctg caggtcgtcg acaagcagct caccgtcgaa
     3421 gagtacgggt acgcgttgcg caaggacgag ccgcagcttc tgcagcagtt caacgatgcg
     3481 ctgaagaagc tcaaggccga cggcacctac gacaagctgg tcgcgaagta cctctgatcg
     3541 ctttctgcgc gtccgcgcga tggatttcga cttctcgccg gtatgggccg gctggcgcga
     3601 gctcctgcat ggcacgttga tcacgatcga ggtgacggcc ggggcgatcg tgctcggctg
     3661 cctgatcggt ctcctgatcg gactcggccg gctggatgcg cgccggcgcg tcgtctatgc
     3721 cgtgtgcacg gcgtacgtgt cgttcattcg cggcacgccg ctgctcgtgc agctcttcat
     3781 ctggtttttc ggcctgccgc acttcggcat caacctgccc gcgttcgtgt gcggcgtgct
     3841 cggcctcggc atctacagcg gcgcgtacgt gtccgaggtc gtgcgcggcg cgatccgctc
     3901 gatcgaccgc gggcagatgg aagcggcgcg ctcgctcggc atgtcctatc cgatggcaat
     3961 gcgccagatc gtcattccgc aggcgatcgt gcggatgatc ccgccgctcg gcaacgagtt
     4021 cgtcgcactc atcaagaatt cgtcgctggt ctcgcttttg acgatcaccg acgtgatgca
     4081 cgaggggcag aagatcatca gcgtctcgta ccgctcgctc gaggtgtatc tcgccatcgc
     4141 actcgtctat ttcgtgctga ccaacctcac cgggctcgcg ctgcgcaccg tggagcgccg
     4201 gcttggcgtg ggtgggctcg tgcaatgagg cccgaggcgt cgccgcagcc catcgtgagc
     4261 gcgcgcggca tcacgaagtc gttcggtccg ctcgcggttc tgaagggtgt cgactgcgac
     4321 gtgatgccgt cgcaggtcag cgtggtgatc ggcccgagcg gttccggcaa gagcacgctc
     4381 ctgcgctgct gcaacgggct cgaagtggcc gacggcggca cgatcgatat ctgcggccag
     4441 cggctcgtcg accacggaac gatgcttcgc gatcccgcgc tcaatgcatt gcgcatgcag
     4501 gtcggcatgg tgttccagtc gttcaacctg tttccgcatc tgtcggtcct cgggaacctg
     4561 acgatcggcc cgcgcaagct gcgcggcctt tcgatcgcgg cggcgcaggc cgaagcgcgc
     4621 gcgctgctcg acaaggtggg gctgcgagac aagatcgacg cctatccgtc gacgctgtcg
     4681 ggcggacaga agcagcgcgt cgccatcgca cgcgcactgg cgatgcggcc gcaggtgatg
     4741 ctgttcgacg aaccgacgtc ggcgctcgac cccgagctcg tcggcgaagt gctgcaggtg
     4801 atgaagaacc tcgcgcgcga gggcatgacg atgctcgtgg taacgcatga aatgggattc
     4861 gcgcgcgaag tcgccgacgt cgtgtcggtc atggatcacg gcgtcgtgct cgaatcgggg
     4921 ccgccggcgc aggtcttcga cgaccctgcg catccgcgca cacgcgcctt cctgcaggcg
     4981 gtgatcacgc gcggcggacc gcggcgctcc gtcgcaccgg cggcgacgtc cgatgcctcg
     5041 atcgtcgact cattgatcgt cgacgatcac tagcgcgctg gtcggcggcc gtgttcccga
     5101 atcagactcc cgcgtaccac tggtagccct ggtcctccca gtagccaccc ttcccctcgc
     5161 cgatgcccgc gaagctctcg acgagctcga ggcgcatcac gtacttcgca tgcttgtacc
     5221 ccaactggcg ctcgacgcgg agccggatcg gcgcgccgtt cttcacgggc agcggcgcgt
     5281 cgttcaacgc atacgcgagc agcgtctgca catggtaggc gtcgtccatg tcgatgctct
     5341 cgtaatacgg cgacgtgccg tcctgctcca tgggatcggc gcagtggaac acgacgtagc
     5401 gcgcgcgttc cgatggctcg acggcagcga gcacggcgga cagtttcgcc ccctgccatt
     5461 tgccgatcgc gctccagcct tcgacgcaat cgtggcgcgt gatctgggtg cggctcggca
     5521 gtgcgcgcag ctcggcgagc gtgaagcggc gcggcttcgc gacgaggccg cccacgtcga
     5581 gcgcgtagtc ggcgaagtga ttggcggcta gtgccgtgta ctgcgcattg tcgggttcgg
     5641 ccgtcccgtt gctgcgaaac gccggcgaca ggtcggcagg cgtgaattcc tgcgccatcg
     5701 ccttgcgtcc ggtgacgagc ttcgccgcgg cgtaattgag cggctcggcc gcgccgagca
     5761 gcttcgggaa ccagtcgctc tgcgacagcc ggtcgcagcc ggcaacaccg agcgcgccga
     5821 gcgaggccgc gagccgccca aggaaacggc gacgcgcgcc gccaccgagc tcatccatgc
     5881 tgcttctcct gcgggatgcg atagcgcccg gtgatcatcg agcgcaggtt gttccagaaa
     5941 cccgtcacga tcacctcgaa cacgtggatc agcacgaacg ccacgagcag ccacgcaacg
     6001 atgaaatgga tcgagcgcac cgtctgccgg ccgccgaacc agccgaccca gccgccgaac
     6061 agcgtgtcga gccgcggtga catgccgagg cccatcagca cgatcagcgg cagcagcacg
     6121 aagatcaccg tcaggtaagc gaatttctgc agcacgttgt agcgtttcgc ggcctcgccc
     6181 gccggatgcc ggaaatgcag atgatcgacg atcgacgtcc cgatgccgcg gatgtcgccg
     6241 cgcgtgggca gcaggtcgcg cgcgagatgg cggctcgcaa tcgtgtacgc gatgaagcac
     6301 aggccgttga tcagaaacac ccacgcgaag aagaagtgcc acgagcgggc catcgcgagc
     6361 cactgatggc tcggtatcgt gagccacgac gggaagccct gcgcgtacat ccttgcatcg
     6421 tcggtcgaaa gtcccagcac gccggtcgtg atgaactcgt ggccgaagag gcgcgtgtag
     6481 ccaaccggct tgccttgcgc atcgtcgcgc gcgccgattt cgagccaggc gggccgtccg
     6541 ctgtacgacg aatccccgac atacagcgcg ggatgcgcgt tgaagatctg cagcccgctc
     6601 atcagcatca gcgccagcgc gatcgcgttg atccagtgca tgacgcgcac cgcgagcgca
     6661 tggcggtaat agagcgcccg cgatggcgca gccgccacgc tcggtgcagg ctcgaggccc
     6721 ggtacggcgg tattcgctt
//
LOCUS       DASSVX010000197         4854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1458101, whole genome shotgun sequence.
ACCESSION   DASSVX010000197 DASSVX010000000
VERSION     DASSVX010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458101"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFO53_08305"
     CDS             <1..338
                     /locus_tag="VFO53_08305"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET8876315.1"
                     /translation="TEDRDVIAPAIEAARAAGALVTGPIPADTVFVPGIAARFDAVVA
                     MYHDQGLPVLKAASFGHGVNVTLGLPFIRTSVDHGTALDLAVDAARARAADVGSLVGA
                     VDLAIALAG"
     gene            338..1129
                     /gene="rsmA"
                     /locus_tag="VFO53_08310"
     CDS             338..1129
                     /gene="rsmA"
                     /locus_tag="VFO53_08310"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET8876316.1"
                     /translation="MQGHQPRKRFGQNFLADPHYVSRIVEAIDPQPGDKVVEIGPGLA
                     ALTGALIERAGRIAAVEIDRDLAARLRERFTADRLALHEADALAFDFSTLGDRLRVVG
                     NLPYNISSPLLFHLASFEAGLRDIHVMLQREVVARMTAAPGTPDYGRLTVMLQTRFRV
                     SRLFTVPAGAFRPAPKVESALARLVPLGEERPRIVDEALFTRIVAAAFGQRRKTLRNA
                     LSMLADEVSLQGAGIDPSARGETLSVDDFVRLTNGIAPLHGMPPA"
     gene            complement(1086..1592)
                     /locus_tag="VFO53_08315"
     CDS             complement(1086..1592)
                     /locus_tag="VFO53_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8876317.1"
                     /translation="MATPTETRSPWIVAARTRPSLGQSTYIRCLSTRLSLFDKRAGGN
                     TFEGGIAMHEDDTDVPTGDEDFLELDEPARPLMIGKRVVNDRTLYCISRELAIARFRS
                     LLLQQQVARRMGTTASAISRLENAVGPRPSLTTLERYADAVDCYLDIRLIPLFTLEAF
                     HAMARYRS"
     gene            complement(1802..2380)
                     /locus_tag="VFO53_08320"
     CDS             complement(1802..2380)
                     /locus_tag="VFO53_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET8876318.1"
                     /translation="MLNNALAYLIDVFFGLVTYTLLLRLVMQLLRAPFRNPLGQAVMA
                     LTDWIVKPLRKVLPGYRGVDWATVVSAIVFQFAWLLALQFTFGRAFALFGPGIAFLLL
                     ATVIALVKALLWLVIIVVFAQAILSWVAPDGPLAGVLNALTFPVLRPVRRVVPPIGGM
                     LDLSPLIVIVLAQLLLITIVPWLESGATSLFI"
     gene            complement(2384..3202)
                     /gene="proC"
                     /locus_tag="VFO53_08325"
     CDS             complement(2384..3202)
                     /gene="proC"
                     /locus_tag="VFO53_08325"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET8876319.1"
                     /translation="MKATFIGGGNMASAILGGLVARGADPAEICVVEPQDAQRKRLVE
                     RFTGLRAYPEVTADSIAGVDLVVFAVKPQQMREAARAVAPHVAQVPAVLTIAAGVRCA
                     DLSRWLGGYPRIVRAMPNTPALVGAGISVLYAMPNAIDAAMLAAPVLEACGEILWCEH
                     ERDLDAVTAVSGSGPAYVFFFLEALEQAAIELGLTAPEARRLAYATFEGSMRLARGSG
                     EAPATLRANVTSKGGTTAAALDVLDAGAVRAGFIAAVKAAAARATALGDELAKD"
     gene            complement(3199..3897)
                     /locus_tag="VFO53_08330"
     CDS             complement(3199..3897)
                     /locus_tag="VFO53_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET8876320.1"
                     /translation="MGQHQKAWQATRQRIADAARAAGRNPDAVRLVAVSKTFSAAAVR
                     DVHGLGQCAFGENYVQEALDKRAALADLPGVIWHLIGPLQANKARVAAEAFDWVETID
                     RARIAGRLSSARSSFVPPLNVLVQVNVSGEATKHGVAPEAAVELARYVATLPNLRLRG
                     IMGIPEPTDDLTRLRSQFRALRGCYDACRDEGMALDTLSMGMSDDLEVAIAEGSSEVR
                     IGRAIFGERARSET"
     gene            4144..>4854
                     /locus_tag="VFO53_08335"
     CDS             4144..>4854
                     /locus_tag="VFO53_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET8876321.1"
                     /translation="MDITELLAFAVKNKASDLHLSSGLPPLIRVHGDIRRINLPPMEH
                     EDVHAMVYDIMSDAQRKMYEEFLECDFSFAVPNLARFRVNAYNQQRGAAAAFRTIPSK
                     VLSLEDLNAPKVFADLTDRPRGLILVTGPTGSGKSTTLAAMVNHVNENEFGHILTIED
                     PIEFLHESKKCLINQRELGPHTLSFPNALRAALREDPDYILVGEMRDLETIRLAMTAA
                     ETGHLVFGTLHTSSAAKTV"
BASE COUNT          846 a         1666 c         1583 g          759 t
ORIGIN      
        1 gcaccgagga tcgcgacgtg attgcacccg cgatcgaagc ggcccgcgcg gcaggcgctc
       61 tcgtgacggg gccgatcccg gccgatacgg tgttcgtgcc cggcatcgcg gcgcgcttcg
      121 atgcggtcgt cgccatgtac cacgaccagg gattgccggt gctgaaagcg gcgagcttcg
      181 ggcacggcgt caacgtgacg ctcggattgc cgttcatccg cacatcggtc gaccacggca
      241 cggcgctcga tctcgcggtc gatgcggcgc gcgccagggc ggccgacgtt ggcagcctcg
      301 tgggcgccgt cgatctcgcg atcgcgctcg cgggttgatg cagggacacc agccgcgcaa
      361 gcgcttcggc cagaacttcc tcgccgatcc gcattacgtc tcgcgcatcg tcgaggcgat
      421 cgacccgcag ccgggcgaca aggtggtcga gatcggtccg ggactcgcgg cgctgaccgg
      481 cgcgctgatc gaacgcgcgg gacgcatcgc ggcggtcgag atcgatcgcg atctcgcggc
      541 gcggctgcgc gaacggttca cggccgatcg ccttgcgctc cacgaggccg atgcgctcgc
      601 attcgacttc tcgaccctcg gcgaccggct gcgcgtcgtc ggcaacctgc cgtacaacat
      661 cagctcgcca ctgctgttcc acctcgcatc gttcgaggca ggattgcgcg acatccacgt
      721 gatgctgcag cgcgaagtcg tggcgcggat gaccgcggca cccggcacgc cggactacgg
      781 acggctgacg gtgatgctgc agacccgctt tcgcgtgtcg cgcctgttca cggttccggc
      841 gggcgcgttt cgtccggcac cgaaggtgga atcggcgctg gcgaggctgg tgccgctcgg
      901 cgaagagcgc ccgcgcatcg tcgatgaggc gctttttacg cgaatcgttg ccgcggcgtt
      961 cggccagcgg cggaagacgt tgcgcaacgc attgtcgatg ctcgccgatg aggtgtcgct
     1021 gcagggtgcc ggcatcgacc cctcggcgcg cggcgagacg ctctccgtcg acgatttcgt
     1081 gcgactcacg aacggtatcg cgccattgca tggaatgcct ccagcgtgaa cagtggaatg
     1141 aggcggatgt cgaggtagca atcgacggcg tcggcgtagc gctcgagcgt ggtcaggctg
     1201 gggcgcgggc cgacagcgtt ttccaaccgg gagatggcac tcgccgtcgt tcccatccgc
     1261 ctggcgacct gctgttgcag cagaagcgag cgaaagcgcg cgatcgcgag ctcgcggcta
     1321 atgcaataga gggtcctgtc attcaccacc cgcttgccga tcatcaatgg tcgcgccggc
     1381 tcgtcgagtt cgaggaaatc ttcgtcgccg gtcggcacgt cggtgtcgtc ctcgtgcatc
     1441 gcgatgcccc cttcgaatgt gttcccgcct gcgcgcttat caaataggct aagccgcgtg
     1501 gacaagcacc tgatataggt cgattggccg agcgaaggac gtgttcgcgc tgcgacgatc
     1561 cacggtgagc gggtctcggt cggggtagcc atacgcgttt cacttagcag aaatgctaag
     1621 tgaaacgagg gaagcacacc cccaccggcc gccgtcaatc gcgcgctgct tgggaatcgg
     1681 ccagtccgcc gttttgccgg ctcgctcgac tacgaggaat gcgcgaagca cttgctgcag
     1741 atcgtcgaga cgaagaagac ggagctccag ttcctgccgg catccgccga agccgcctga
     1801 ttcagatgaa aagactcgtc gccccgctct cgagccacgg cacgatcgtg atcagcagca
     1861 gctgcgcgag cacgatcacg atcagcggcg acagatcgag catccccccg atcggcggca
     1921 cgacgcggcg caccggccgc agcaccggaa acgtcagcgc gttcagcacg ccggcgagcg
     1981 gtccgtcggg cgcgacccac gacaggatcg cctgtgcgaa cacgacgatg atgacgagcc
     2041 acagcagcgc cttgacgagc gcgatcaccg tggcgagcag caggaacgcg atgccgggcc
     2101 cgaacagcgc gaaggcgcgg ccgaacgtga actgcaacgc aagaagccac gcgaactgga
     2161 agacgatcgc cgacacgacc gtcgcccagt cgacgccgcg ataaccggga agcaccttgc
     2221 gcagcggctt cacgatccag tcggtcagcg ccatgaccgc ctggcccagt ggattgcgaa
     2281 acggcgcgcg caggagctgc atgacgagcc gcagcagcag cgtgtacgtc acgagcccga
     2341 aaaagacgtc gatcaggtac gcgagcgcgt tgttgagcat cgatcaatcc ttcgcgagtt
     2401 cgtcgccgag cgcggtcgcg cgtgccgcgg ccgccttcac tgcggcgatg aagccggcgc
     2461 gcacggcgcc ggcatcgagg acgtcgagcg cggccgccgt ggtcccgccc ttcgacgtca
     2521 cgttcgcgcg aagcgttgcc ggcgcttcgc cgctgccgcg cgcaaggcgc atgctgccct
     2581 cgaacgtcgc gtacgcgagg cgccgcgctt ccggcgcggt caagccgagc tcgattgccg
     2641 cctgctcgag cgcctcgaga aaaaagaaga cgtacgccgg cccgctgccc gataccgcgg
     2701 tgaccgcatc gagatcccgc tcgtgctcgc accacaggat ctcgccgcag gcttcgagca
     2761 cgggcgccgc cagcatcgcc gcatcgatcg cattcggcat cgcgtacagc acgctgatgc
     2821 cggcaccgac gagcgccggc gtattcggca tcgcgcgcac gatgcgcgga tagccgccga
     2881 gccagcgcga cagatcggcg caacgtacgc ccgcggcgat cgtcagcacc gcgggaacct
     2941 gcgcgacatg cggtgcgacg gctcgcgcgg cctcgcgcat ctgctgcggc ttcaccgcga
     3001 acacaacgag atcgacgccg gcgatcgagt cggcggtgac ctcgggatac gcgcgcaggc
     3061 cggtgaagcg ctcgacgagc cgcttccgct gcgcgtcctg cggctcgaca acgcaaattt
     3121 ccgcgggatc ggcgccgcgt gcgacgagac cgccgaggat cgcggaggcc atgttgcccc
     3181 cgccgatgaa cgtcgctttc acgtttcgct ccttgcccgc tcgccgaaga tcgcacgccc
     3241 gatgcgcacc tcgctggaac cttccgcgat ggcgacctcg aggtcgtcgg acatccccat
     3301 cgacaatgtg tcgagcgcca tgccttcgtc gcggcacgcg tcgtaacaac cccgaagcgc
     3361 ccggaactgc gaccgcaacc gcgtgaggtc gtccgtcggc tcgggaatgc ccatgatgcc
     3421 gcgcaggcgc aaattcggca gtgttgcgac gtagcgcgcg agctcgacgg ccgcctccgg
     3481 cgcgacaccg tgcttcgtcg cttcgccgct cacgttcacc tgcacgagga cattgagcgg
     3541 agggacgaag ctcgaacgtg ccgacgaaag ccgcccggcg atgcgcgccc ggtcgatcgt
     3601 ctcgacccag tcgaacgcct cggccgccac ccgcgccttg ttcgcctgca gcgggccgat
     3661 cagatgccat atcacgccgg gaaggtcggc gagcgccgcc cgcttgtcga gcgcctcctg
     3721 cacgtagttc tcgccgaagg cgcactggcc cagaccgtgc acgtcgcgca cggccgcggc
     3781 ggaaaacgtc ttcgacaccg cgacaagccg caccgcatcc gggttgcggc cggcggcccg
     3841 ggcggcgtct gcgattcgct ggcgcgtcgc ttgccacgct ttctggtgct gtcccataat
     3901 cgagcgcaat tagaccatcg aaccatggca cgtgtattgc atactacagt ccgtttgagc
     3961 atcgatcgct cgtcgcgcga ggtcccggga ccgatggaaa ctaccgaaaa cacgtgcaag
     4021 caagctgcta cggcgctttc gcacgaaaac atcgcatccc ccgtgggcag cccattcgcg
     4081 gcgacgcccc tcaaaaccgt cggtcgcgac gcgattcgct gcgtcacgat ccggggctga
     4141 tccatggata tcaccgagct cctcgcattc gcggtcaaga acaaggcttc cgacctgcac
     4201 ctgtcgtcgg gactgccgcc gctgatccgc gtccatggcg acatccggcg catcaacctg
     4261 ccgccgatgg agcacgagga cgtgcatgcg atggtgtacg acatcatgag cgacgcgcag
     4321 cgcaagatgt acgaggagtt cctcgaatgc gacttcagct tcgcggtgcc gaacctcgcg
     4381 cgctttcgcg tcaacgcgta caaccagcag cgcggtgccg ccgcggcgtt ccgcacgatt
     4441 ccgtcgaagg tgctgtcgct cgaggacctc aacgcgccga aggtcttcgc cgatctcacc
     4501 gaccgtccgc gcggcctgat cctcgtcacc gggcccacgg gctcgggcaa gtcgacgacc
     4561 ctggcggcga tggtcaatca cgtcaacgag aacgagttcg gccacatcct cacgatcgag
     4621 gacccgatcg agtttttgca cgagtcgaag aagtgcctga tcaaccagcg cgagctcggt
     4681 ccgcatacgc tgtcgtttcc gaatgcgctg cgggcggcgc tgcgcgagga tcccgactac
     4741 atcctggtcg gcgaaatgcg cgacctcgaa acgatccgcc tcgcgatgac ggccgccgag
     4801 acgggccacc tcgtgttcgg cacgctgcat acgagctcgg cggcgaaaac ggtc
//
LOCUS       DASSVX010000198         7397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_674598, whole genome shotgun sequence.
ACCESSION   DASSVX010000198 DASSVX010000000
VERSION     DASSVX010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674598"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1785)
                     /locus_tag="VFO53_08340"
     CDS             complement(<1..1785)
                     /locus_tag="VFO53_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876322.1"
                     /translation="MKRRAPSWEGQNRRLVLATLGVVALAPHFARAQSRSNGGAVEKT
                     LRIAFPVAETGFDPQATSDLYSDHVQRAIFETLYGFDYLARPYKRVPRTAAALPKIED
                     GGRTWTIEVRRGIHFADDAAFGGRKRELTAADYVYAWKRLLDPRMRSPFSWYLQGKLV
                     GADAVLDAAKKAGKLDYDAPIEGLAALDRYTIRIRLAEPDYILFGYLCSSPMAAVARE
                     VVERYGDTNGWTMDHPVGTGAFMLKSWRRGQQIVLEANPGFRDERFPEPPDAGDRERF
                     ASLIGKRLPLVDRVEIAIMEESNPRLLAFDSNALDYVNLPPELTDHALDAANRLKAPY
                     VARGVTLQRLTQPAMQYAYFNMQDPVVGGYDNAKIALRRAIAMAFDTPELIKVVYEGQ
                     ALAATQPIPPNVPGHDDALNVAAAYNPAEAKALLDKFGYVDRNGDGLRELPDGKPLSL
                     TMASTPTTRDREIDEIWQRNLKAVGIRVEFMKQKWPDLLKMGKAGQLQFWRIGWINAY
                     AEGDAFMQLLYGGNIGQTNYSRFDLPAYDALYRKSRTLPDGAERNAVYRKMSELVGAY
                     APWVLHVYTIEDTLVQPWVRGYRKNAYWE"
     gene            complement(1782..3644)
                     /locus_tag="VFO53_08345"
     CDS             complement(1782..3644)
                     /locus_tag="VFO53_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876323.1"
                     /translation="MNALRIARSAGVALFASLVAAAAPVASAQGPSTKTLHVAFPVAE
                     TGFDPQAAGDIYSNYVNRAIFDPLYRYDYLARPYKLVPNTAAGMPQITDDGKTWTIEV
                     RKGIHFADDPVFKGKRRELTAADYVYAIKRILDPKMRSNSLQMVDGRFVGADAVVANA
                     KATGKFDYDAPIEGLQAIDRYTLRFKLVFADYELLSNLTTTALSAVAREVIEAHADGN
                     GWAMANPVGTGPYRLKDWRRGQRIVLEANPGYRDERYPTPSSAADQALARGLAGRRLP
                     LVANVDISIIEEAQPRLLAFQQKQLDFVAVPVNIVTNVLDADNRLKPEFANAGVRLER
                     AIQPAIVYMYFNMDDPVVGGYTPPQIALRRAIAIGYDVNEEIRVLRQNQGLPATQVVP
                     PNMTGHDPHYDGRLKYDPAAARALLDRFGYVDRNGDGFRELPDGKPLMLRVGSGTTEQ
                     DRQFEELWKRCLDALGIRAEFVKQKWPDMLKMARLGQLQMWQVGNINTTPDGFGFFGL
                     LYGRNAGFSNLSRFALPEFDRLYEKARAMPDSPERLAVEGKMSDLVNAYAPWVLTAFR
                     YENVVVQPWLRGFKYNPTFQYPFAYLDIDRSGGAAGSGRDAASARTSRDAKDAS"
     gene            complement(3644..4123)
                     /locus_tag="VFO53_08350"
     CDS             complement(3644..4123)
                     /locus_tag="VFO53_08350"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET8876324.1"
                     /translation="MATLHDFTVDDIEGHPVSLDRYKGKVLLVVNTASKCGFTPQYKG
                     LESLYRKYRDRGLEVLGFPCNQFGAQEPGSEQEIAAFCETSYDVTFPMFAKVDVNGDA
                     AAPLYRYLKREKPGLLGTEAIKWNFTKFLVDRDGSVVARYAPNDTPESLEADVAKAL"
     gene            4199..4663
                     /locus_tag="VFO53_08355"
     CDS             4199..4663
                     /locus_tag="VFO53_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET8876325.1"
                     /translation="MASRQSAGIVMYRRRGSAIEVLLVHPGGPFWAKKDAGAWSIPKG
                     EFGAGEAPAIAARREFIEEIGHDPGAALVPLGIVTQSRAKVVHAFAVEGDVDPVTIRS
                     NAFEIEWPPRSGHMQAFPEVDRAAWFTLDEARAKIVAGQRAILDALAVISRR"
     gene            complement(4677..5387)
                     /locus_tag="VFO53_08360"
     CDS             complement(4677..5387)
                     /locus_tag="VFO53_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HET8876326.1"
                     /translation="MTTECLFRDDAYLTSATARVIAVHERGVELDRTLFYPQGGGQMG
                     DTGALTRDNGERLGVVDTRKGDAPDTVLHVLADGSPLPEPGEPLTLDIDWARRYALMR
                     LHTALHVLSCVVVAPVTGGNIAPDKARLDFDIDMSLLDAAKIERETNALIARAIATET
                     VWISDEELDARPELVKTMSVAPPRDSGRVRLLRIPGIDLQPCGGTHVANIGEIGAIRV
                     LKIKSEGRRNRRVEIALR"
     gene            5466..6920
                     /locus_tag="VFO53_08365"
     CDS             5466..6920
                     /locus_tag="VFO53_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HET8876327.1"
                     /translation="MNIPNASPALYNEDLAPATTRNWGAFSIFNVWTSDVHSLWGYYL
                     AASLFLLCGSFTNFIIAIGLGSLVIFFLMNLVGYAGVKTGVPFPVLARASFGVWGANV
                     PALVRAIVACFWYGAQTSLASGAIVALLTRTDAFMSFHTNVHFLGHSALEVICFVIVW
                     ALQLLIIQKGMETVRRFQDWAGPAVWVMMLILAIGLVIKAGGFSFDHGIPQDVLLAKT
                     KDAGVPGTPGSTAALMAVAATWITYFAALYLNFCDFSRYAPDKATLRKGNLWGLPINL
                     VLFSLVAGITTLAAYTVYGEVLLHPEHISAKFDSWFLALLAALTFAVATLGINVVANF
                     VSPAFDFSNVFPKAIDFKKGGYIAAAIALVLYPFAPWEGSAAVFVGGIGATMGPLFGV
                     IMVDYYLIAKSEVNVPELYRENGEYRYQGGFNIAAFVAAGIGALFSSILPNFTTLLPS
                     WWGVYGWFFGVAIAGVAYYALSMLMPRPVAQAAA"
     gene            complement(6993..>7397)
                     /locus_tag="VFO53_08370"
     CDS             complement(6993..>7397)
                     /locus_tag="VFO53_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET8876328.1"
                     /translation="SGGAGAGADFDGASVVQTHMTNSRLTDPEVLEWRFPVRLDAFSI
                     RRGSGGAGAHRGGDGAVRRVRFLEPMTAVMLANHRRVAPFGVAGGAPGAIGRNWIERA
                     GGAREDYGATFKVEMNAGDVFVVETPGGGGYG"
BASE COUNT         1143 a         2439 c         2470 g         1345 t
ORIGIN      
        1 ctcccagtac gcgttcttcc ggtagccgcg cacccacggc tgcacgagcg tgtcctcgat
       61 cgtgtagacg tgcagcaccc acggcgcgta agcgccaacg agctccgaca tcttccggta
      121 gaccgcgttg cgctcggcgc cgtcggggag cgttcgcgat ttcctgtaaa gcgcgtcgta
      181 ggcgggcagg tcgaagcgcg aatagttggt ctggccgatg ttgccgccat agagcaactg
      241 catgaacgcg tcgccctcgg cgtatgcgtt gatccagccg atgcgccaga actgcagctg
      301 ccccgccttg cccatcttga ggaggtcggg ccacttctgc ttcatgaact cgacgcggat
      361 gcccaccgcc ttcaggttgc gctgccagat ctcgtcgatc tcgcgatcgc gcgtggtcgg
      421 cgtcgatgcc atcgtcagcg acagcggctt cccgtcgggc agctcgcgca agccgtcgcc
      481 gttgcgatcg acgtaaccga acttgtcgag cagcgccttc gcctcggccg ggttgtaggc
      541 agcggcgacg ttcagcgcat cgtcgtgccc gggaacgttc ggcggaatgg gttgcgtcgc
      601 cgcgagcgcc tgcccttcgt agacgacctt gatcagctcc ggcgtgtcga acgccatcgc
      661 gatcgcacgc cgcagcgcga tcttcgcgtt gtcgtaaccg ccgacgacgg gatcctgcat
      721 gttgaagtac gcgtactgca tcgcgggctg cgtcagccgc tgcagcgtca cgccgcgggc
      781 gacgtacggc gccttcagcc ggttcgccgc gtcgagcgca tggtcggtga gctcgggcgg
      841 caggttcacg taatcgagcg cattgctgtc gaaggcgaga agacgcgggt tcgactcttc
      901 catgatcgcg atctcgacgc gatcgacgag cggcaggcgc ttgccgatca gcgacgcgaa
      961 gcgctcacgg tcgcccgcat cgggcggctc gggaaagcgc tcgtcgcgaa agcccgggtt
     1021 cgcctcgagc acgatctgct ggccgcgccg ccacgacttc agcatgaacg cacccgtgcc
     1081 gaccgggtga tccatcgtcc agccgttggt gtcgccatag cgttcgacga cctcgcgcgc
     1141 caccgccgcc atcgggctcg agcacaggta gccgaacagg atgtaatcgg gctcggcgag
     1201 ccggatacgg atcgtgtagc ggtcgagcgc ggcaagtcct tcgatcggcg cgtcgtagtc
     1261 gagcttgccg gccttcttcg ccgcatcgag cacggcgtcg gcgccgacga gcttgccctg
     1321 caggtaccag gaaaacggcg agcgcatgcg cgggtcgagc aggcgcttcc atgcgtagac
     1381 gtaatcggcg gcggtgagct cgcgcttgcg gccgccgaac gccgcgtcgt cggcgaaatg
     1441 aatcccgcgc ctgacctcga tcgtccacgt gcgtccgccg tcttcgatct tcggcagcgc
     1501 ggcggcggtg cgcggcacgc gcttgtacgg ccgcgccagg tagtcgaagc cgtagagcgt
     1561 ttcgaaaatg gcgcgctgca cgtggtcgga atagaggtcg ctcgtcgcct gcggatcgaa
     1621 gcccgtctcg gccaccggaa aggcgatgcg cagcgtcttc tccacggcgc cgccgttcga
     1681 gcgcgattgc gcccgcgcga aatgcggcgc gagcgccacg acgccgagcg tggcgagaac
     1741 gagacggcga ttctggcctt cccacgatgg cgcgcggcgc ttcacgacgc gtccttggcg
     1801 tcccgcgacg ttctggcgga cgccgcatcg cgtcccgagc cggctgcgcc gccggaacgg
     1861 tcgatgtcga gatacgcgaa cggatactgg aacgtggggt tgtacttgaa tccgcgcagc
     1921 cacggctgca cgacgacgtt ctcgtagcgg aacgcggtca gcacccatgg cgcgtacgcg
     1981 ttgacgaggt ccgacatctt cccttcgacc gcgagccgct cgggactgtc gggcatcgcg
     2041 cgcgccttct cgtagagccg gtcgaattcg ggcagcgcga aacgcgacag gttcgagaag
     2101 ccggcgttcc ggccatagag caggccgaag aagccgaagc cgtccggcgt ggtgttgatg
     2161 ttgccgacct gccacatctg caactggcca agacgcgcca tcttcagcat gtccggccat
     2221 ttctgcttga cgaactcggc acggatgccg agtgcgtcga ggcagcgttt ccacaattcc
     2281 tcgaactggc ggtcctgctc ggtcgtgccc gaccccacgc gcagcatcag cggcttgccg
     2341 tcgggcagct cgcgaaaacc gtcgccgttg cgatcgacgt agccgaagcg gtcgagcagc
     2401 gccctggccg ccgcgggatc gtacttgagg cggccgtcgt agtgcgggtc gtgaccggtc
     2461 atgttgggcg gcacgacctg ggttgccggc agtccctggt tctggcgcag cacgcggatt
     2521 tcttcgttga cgtcgtagcc gatcgcgatc gcgcggcgca acgcgatctg cggaggggtg
     2581 tagccgccga cgacgggatc gtccatgttg aagtacatgt agacgatcgc cggctggatc
     2641 gcgcgttcga gccgcacacc cgcgttcgcg aactcgggct tcaggcggtt gtcggcatcg
     2701 aggacgttcg tgacgatatt gacgggcacc gcgacgaagt cgagctgctt ctgctggaat
     2761 gcgaggagcc gcggctgcgc ttcctcgatg atgctgatgt cgacgttcgc caccagcggc
     2821 agccggcggc cggcgagccc cctggcgagc gcctggtcgg cggcgctgct cggtgtggga
     2881 tagcgctcgt cgcgatagcc gggattcgcc tcgagcacga tgcgctggcc gcggcgccag
     2941 tccttgagcc ggtagggacc ggtgccgacc ggattggcca tcgcccagcc gttgccgtcg
     3001 gcatgcgcct cgatcacttc gcgcgcgacg gccgacagcg cggtcgtcgt gagattcgac
     3061 aatagctcgt aatcggcaaa cacgagcttg aatcgcagcg tgtaacggtc gatcgcctgc
     3121 agcccttcga tcggcgcgtc gtagtcgaac ttgccggtcg ccttcgcgtt cgcgacgacg
     3181 gcgtcggcac cgacgaagcg cccgtcgacc atctgcagcg agttcgaacg catcttcgga
     3241 tcgaggatgc gcttgatcgc gtagacgtaa tcggcagccg tcagctcccg ccgcttgccc
     3301 ttgaataccg gatcgtcggc gaaatggatg cccttgcgga cctcgatcgt ccacgtcttg
     3361 ccgtcgtcgg tgatctgcgg catcccggcg gcggtgttgg gcacgagctt gtacgggcgc
     3421 gcgagatagt cgtagcgata cagcggatcg aagatcgcgc gattgacgta gttcgaatag
     3481 atgtcgcccg ccgcctgcgg atcgaatccg gtttcggcga ccgggaaggc gacgtgcagc
     3541 gttttcgtcg acggcccctg cgcgctcgcg acgggtgcgg ccgcggcaac gagcgacgcg
     3601 aagagcgcga cgccggcgga tcgcgcgatc cgtagcgcgt tcatcacaac gccttcgcga
     3661 cgtcggcttc gagcgactcg ggcgtatcgt tcggcgcgta gcgcgcaacg acgctgccgt
     3721 cgcgatcgac gaggaacttg gtgaagttcc acttgatcgc ctcggtgccg agcagccccg
     3781 gcttttcgcg cttcaggtag cggtagagag gcgccgcggc gtcgccgttc acatcgacct
     3841 tggcgaacat cggaaacgtg acgtcgtagc tcgtctcgca gaaggccgcg atctcctgct
     3901 cgctgccggg ctcctgggcg ccgaactggt tgcacggaaa gccgagcact tcgaggccgc
     3961 ggtcgcgata cttgcggtac agcgattcga ggcccttgta ctgcggcgtg aagccgcact
     4021 tgctcgccgt gttgacgacg agcagcacct tgcccttgta gcggtcgaga ctgaccggat
     4081 gaccctcgat gtcgtcgacg gtgaagtcgt ggagcgtcgc catggtccct cctcgggagg
     4141 tgcgggaaag gtcattatcg cgcgagcgcg cttccgcgcc cagcgtagca tcgtcgacat
     4201 ggcttcgcgg cagagcgccg gcatcgtgat gtaccgtcga cgcgggtcgg cgatcgaggt
     4261 gctgctggtc catcccggcg gcccgttctg ggcaaagaag gatgccggcg cgtggtcgat
     4321 tccgaagggt gaattcggtg ccggcgaggc gcccgccatt gcggcgcgac gcgagttcat
     4381 cgaggagatc ggccacgatc ccggtgcggc actcgtcccg cttggaatcg tcacgcagtc
     4441 gcgagcgaag gtcgtccatg cgttcgcggt cgagggcgat gtcgacccgg taacgattcg
     4501 cagcaacgca ttcgagatcg aatggccgcc gcgctctggc cacatgcagg cgtttcccga
     4561 ggtggatcgc gcggcgtggt tcacgctcga cgaagcgcgg gcgaaaatcg tcgccggcca
     4621 acgagcgatt ctcgacgcac tggcagtgat ctcccggcgc tgaccgcggc cccctgttat
     4681 cgcagtgcga tctccacccg ccggttgcgc cgtccctcgc tcttgatctt cagcacgcgg
     4741 atggcgccga tttctccgat gttggcgacg tgcgtcccgc cgcacggctg aaggtcgatg
     4801 cccgggatgc gcagcagccg cacccgcccg ctgtcgcgcg gcggcgcaac gctcatcgtc
     4861 ttgacgagct cgggccgcgc gtcgagctcc tcgtcgctga tccagaccgt ttccgttgcg
     4921 atggcgcgcg cgatcagcgc gttcgtctcg cgctcgatct tcgccgcgtc gagcaggctc
     4981 atgtcgatgt cgaaatcgag ccgcgccttg tcgggcgcga tgttgccgcc cgtgacgggt
     5041 gcgacgacga cgcacgacag cacgtgcagc gcggtgtgca gccgcatcaa tgcgtagcgg
     5101 cgcgcccagt cgatgtcgag cgtcagcggc tcgccgggtt cgggcagcgg cgagccgtcg
     5161 gccagaacgt gcagcacggt atcgggcgcg tcacccttgc gcgtgtcgac cacgccgagg
     5221 cgctcgccgt tgtcccgcgt caaggcaccg gtgtcgccca tctggccgcc accctgcggg
     5281 tagaagagcg tgcggtcgag ctcgacgccg cgctcgtgca cggcaatcac gcgtgcggtc
     5341 gccgaggtca gatacgcatc gtccctgaac aggcattcgg tcgtcatcgc attacatttg
     5401 cgttcgcccg cattacactt ccgtacgaaa cggccgatcg taccctacgt tgacggagga
     5461 acgagatgaa cattccgaac gcatcgccgg cgctgtacaa cgaagacctc gcgcccgcca
     5521 ccacgcgcaa ctggggcgcc ttcagcatct tcaacgtctg gacctcggac gtgcacagcc
     5581 tctggggcta ctacctcgcg gccagcctgt tcctgctgtg cggcagcttc accaatttca
     5641 tcattgcgat cgggctcggc tcgctcgtga tcttcttcct gatgaacctc gtcggctatg
     5701 cgggtgtgaa gaccggcgtg ccgtttccgg tgctcgcgcg cgcatcgttc ggcgtgtggg
     5761 gcgccaacgt gccggcgctc gtgcgtgcga tcgtggcctg cttctggtac ggcgcgcaga
     5821 cgtcgctcgc ctccggcgcc atcgtcgcgc tgctgacgcg caccgatgcg ttcatgtcct
     5881 ttcacaccaa cgtccatttc ctcggccatt cggcgctcga ggtgatctgc ttcgtgatcg
     5941 tctgggcgct gcagctgctg atcatccaga agggcatgga gacggtgcgc cgcttccagg
     6001 actgggccgg gccggccgtg tgggtgatga tgctgatcct cgcgatcggc ctcgtcatca
     6061 aggccggcgg cttctcgttc gatcacggca ttccgcagga cgtgctgctc gcgaagacca
     6121 aggatgccgg cgtccccggc acgccgggat cgaccgcggc gctgatggcc gtcgccgcca
     6181 cgtggatcac ctatttcgcc gcgctgtacc taaatttctg cgacttctcg cgctacgcgc
     6241 ccgacaaggc cacgctgcgc aagggcaacc tgtggggatt gccgatcaac ctcgtgctgt
     6301 tctcgctcgt cgcgggcatc acgacgctgg ccgcctacac ggtgtatggc gaggtgctgc
     6361 tgcaccccga gcatatttcc gcaaagttcg acagctggtt cctggcactg ctcgcggcat
     6421 tgacattcgc ggtggccacg ctcggcatca acgtcgtcgc gaacttcgtt tcgcccgcgt
     6481 tcgatttctc gaacgtgttc ccgaaggcga tcgatttcaa gaaaggcggc tacatcgcgg
     6541 ccgcgatcgc gctggtgctc tatccgttcg caccgtggga aggcagcgcg gcggtgttcg
     6601 tcggcggcat cggcgcgacg atgggaccgc tgttcggcgt gatcatggtc gactactatt
     6661 tgattgcgaa aagcgaggtg aacgtgccgg aactctatcg cgagaacggc gaatatcgtt
     6721 accagggcgg gttcaacatc gccgcgttcg tcgccgccgg catcggtgcg ctgttctcga
     6781 gcatcctgcc caacttcacg acgctgctgc cgtcgtggtg gggcgtgtac gggtggttct
     6841 tcggcgtggc catcgccggt gtcgcctact acgcgctgtc gatgctgatg ccgaggccgg
     6901 tcgcgcaggc ggccgcgtag acgacgagat cggggtcaga gctgtaatta ttcggatttg
     6961 cgaataatta cagctctgac cccgatctcc ggtcagccgt aaccgccgcc acctggtgtc
     7021 tcgacaacga acacgtcgcc ggcgttcatc tcgaccttga acgtcgcgcc gtaatcctcg
     7081 cgcgcgccgc cggcgcgctc gatccagttg cgcccgatgg cgcccggcgc gccgcccgcg
     7141 acgccgaacg gtgcgacgcg ccggtgattg gcaagcatca ccgcggtcat cggctcgaga
     7201 aagcgcacgc gcctgaccgc gccgtcaccg ccgcgatgcg caccggcgcc gccgctgccg
     7261 cggcggatcg agaacgcgtc gaggcgcacc ggaaagcgcc actcgagcac ttcgggatcg
     7321 gtgagccgtg agttggtcat gtgcgtttgc accacactgg cgccgtcgaa atcggcgccc
     7381 gcgcccgcgc cgcccga
//
LOCUS       DASSVX010000199         9045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_724659, whole genome shotgun sequence.
ACCESSION   DASSVX010000199 DASSVX010000000
VERSION     DASSVX010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9045
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724659"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VFO53_08375"
     CDS             <1..997
                     /locus_tag="VFO53_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HET8876329.1"
                     /translation="EIVTLITALGTGFGKDEYNVDKLRYHRIIIMTDADVDGSHIRTL
                     LLTFFYRQMPELVERGHIYIAQPPLYKAKQGREDVYLKDDHELKQHLLRVALKGAELV
                     PGEGRPPLSAESLGDVAREYLLAEAVIERLARVVDPAALRAMLSGVRLDLASESTARR
                     SASALQAAIDDTEVAAEARYDATNEVHRIIIVRTHHGTPHATAIDADFLASGDADQIR
                     RAADVLQDLIYPGAVVKRGDKQQAVKSFREALDWLLGEARGSVAIQRYKGLGEMNPGQ
                     LWETTMDPAVRRLLKVQIEDAIGSEEIFSTLMGEQVEPRRAFIESNALGVRNLDV"
     gene            1027..3048
                     /locus_tag="VFO53_08380"
     CDS             1027..3048
                     /locus_tag="VFO53_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET8876330.1"
                     /translation="MRAWFGSPLRGFAALLVTLIAAGVAQAAPPSVEAFFRKPAYGGA
                     TLSPNGRYLAVVAPVGERRGLIVLDLEAKAATRMQSPGDGDVLGAVWQNDKRLVVRIG
                     ELQRASGEPPREAGLVAIDRDGTDSRVVAGIQPRTMAGSSIVEKRFERPWFVRLLRVV
                     EGTNDVLLTARERNAETLDVYRYDTVSGEKTLLTFDSPGAVQHWVVDFDGVPRAALTA
                     DIDHDVSAWYVRKSAGDPWIEVEAAKLGALNSVPLEFDPNGKLLYVSARRNGDDRASI
                     YEYAIDAHAWRKPIVQHPLRDVDGGNASFRSDYRAREILGLAYTDDKPSVAWFDAGYA
                     RVQKSVDATLPDTVNLLQRRGDRFLVVAFSDRDPGEAYLLDTKSMRLEKLFSYEPWID
                     VSAMAAQRWVRYPARDGLTIPALLTVPPNARVKPFPLVVVIHGGPNVEATRWGYDAET
                     QFLVSRGYAVLQPQFRGTLGFGWKLRSAGFRKWGDEMQDDLEDGVKWAIAQGIADPGR
                     VCFYGASYGGYAAAWGAIKNAKLIKCAVSYVGVMSIDYLFDNAQTDLSRLADRSSLMV
                     REIGDPKIERARFRRVNPIDNADKVGVPILLAYGASDRRVPLVHGSDFRNALDRAHKP
                     YEWVVYADEGHGFVKDGNLFDFYGRVDRFLAKYLDASAAPAATAATVGR"
     gene            3087..5255
                     /locus_tag="VFO53_08385"
     CDS             3087..5255
                     /locus_tag="VFO53_08385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET8876331.1"
                     /translation="MPASPVRTLAVAAMLTLATLAHAGGPTRLIVKLKNAAAKAAPTP
                     QARIEKLSAASGLRLAHVRAMALGADVVIAPDADTRSALAAIAADRDVEFVEVDARVH
                     AMQAAPPVNDTFAPQQRYLADTPTAISAYDAWNVTHGSPNIVVAVVDSGYRPHAGIAG
                     RFLPGYDMISEADVANDGDGRDADASDPGDWVLASEASGDCHAHDSVWHGTSVAGIIG
                     ANTNDGSWTAGIDWNARILPVRVLGKCGGFDSDVVDGIAWAAGLSVPGVPPNPTPAHV
                     INLSLGSSRACSAVYTDVAAAAYAHGVTRAIVAAAGNDSQEVSNDNPASCPGYYAIAS
                     TTGVSGKLASFSNFGPGITLSAPGGAANFRTPADSALVLSNSGTTTPLADIVVSEGGT
                     SFSAPMVSATVSLMLAVAPFLTPGQVHDIIVGTAKAFPSTSDCTTDRCGAGIVDVGAA
                     VRAAASASPPPAAVNYQGLWWATPPGSESGWGINFAHQGDVIFATWFTYDATGKAWWL
                     SMTASRSGQNVYSGDLLQTHGPAFSATPFDPNAVTHSTVGHATLTFTDANTAQFVYTV
                     NGIAQTKTLTRELFGTAPACNFGAQPNLALATNYQDLWWAAPAGVESGWGINLTEQSS
                     MIFGTWFTYDVDGSPLWLSVLATNTAPGVFTGDLVRTRGPPFSATPFDPAMVTHTGVG
                     TATFTFSDGSHATFAYTVGGVTQSKPITREVFVNPGTFCQ"
     gene            5353..6831
                     /locus_tag="VFO53_08390"
     CDS             5353..6831
                     /locus_tag="VFO53_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8876332.1"
                     /translation="MHRPRVPVADGSRLVSFNPANEQALGAIATTSAAGFDVLLSSAV
                     DASLAWRDTPAPVRGNAVRRYAQLLREHKDALGTLVSLETGKIKAEGDGEVQEMIDIA
                     DFAVGQSRMLYGKTLHSERPSHRMYEQWHPLGVVGVITAFNFPVAVWAWNAMLACVCG
                     NAVVWKPSPKTPLAALAAAHLADRVVDEQHLPQIFSLALGDDALGRALVADPRVALVS
                     FTGSSRVGQAVAGAVSARFGKVLLECGGNNAVIVADDADADLVIPAVVFGAVGTAGQR
                     CTTTRRLIVHRSRVDDVVTRLANAYAQVRIGDPLQPSTLMGPLIDRGAVDGYLRAIDE
                     ARAAGATIVCGGRIVPGDGFFVEPTIIGGLDSAHPLVQRETFAPILYVLAFDTIDDAI
                     ARNNGVRQGLSSALFTQSLRTAEAFLAASGSDCGIANINIGTSGAEIGGAFGGEKETG
                     GGRESGSDAWRAYMRRQTNTINWSRELPLAQGIRFEIDRPAP"
     gene            complement(6970..7170)
                     /locus_tag="VFO53_08395"
     CDS             complement(6970..7170)
                     /locus_tag="VFO53_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HET8876333.1"
                     /translation="MQRLLIVLGVVMLLVGVAWPWVSKLGLGRLPGDINIERDGWSFH
                     FPLTTGVLVSLVLTLVVWLLRK"
     gene            complement(7205..>9045)
                     /locus_tag="VFO53_08400"
     CDS             complement(7205..>9045)
                     /locus_tag="VFO53_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HET8876334.1"
                     /translation="GEPFKSEGKVLVQPGWLAVYGKEAAGDEPTLTPVKVREIVDAKA
                     APDARATAVRRYEGTEKVDTRSVDVVALTTRPPPRFNEATLLSAMEGAGKSIEDDELR
                     EAMREKGLGTPATRAQIIEGLIAERYVLREGKELIATPKASSLITLLHGLGVPELFSP
                     ELTAEWEFKLAQMEHGALARSEFMHEIVGMTKHIVAQAKNYESDTVPGDFATLSARCP
                     KCGGEVHEQYKKFACVNCDFGFWKIMGGRQIEPSEADTLITERSAGPLDGFRSRIGRP
                     FSAKLKLNDANEVEFDFGPRPGDEEGGSYDFSSLAPLGPCPKCGNRVFETPNAYVCER
                     SVGEDRSCDFRSGRTILQKTIEPVQMTKLLETGKTDLLQFVSARTRRPFSAYLVKQAD
                     GKVGFEFEAKAPGRRGARPMRSAPLRVLGAHPRDRQPVELHAGRYGPYVKHGATNATL
                     PDRDAVDSLSLEQAIALVDEKAGREPRAATRTGAKTRAPRKPRAATPAEPVKAVRRIA
                     GARGGRSEDAVAPAAKRASTRTAPADKRAPAAKPVGAPAAKAAASRASVVKKRAAVKR
                     IAPDAKTGGGTTVTRKGTATSRTTRAKAPSARKTGTSATARKSKRR"
BASE COUNT         1415 a         3082 c         3041 g         1507 t
ORIGIN      
        1 ggagatcgtc acgctgatca ccgcgctcgg cacgggtttc ggcaaggacg agtacaacgt
       61 cgacaagctg cgctaccacc ggatcatcat catgacggat gcggacgtcg acggttcgca
      121 catccgtaca cttctgctga cgttcttcta ccggcagatg ccggaacttg tcgagcgcgg
      181 gcacatctat atcgcccagc cgccgctgta caaggcgaag caaggtcgcg aggacgtcta
      241 tttgaaggat gatcacgaac tgaagcagca tctgctgcgc gttgcgctga agggcgcgga
      301 gctcgttccg ggcgaaggcc gcccgccgct ttcggccgag tcgctcggcg acgtcgcccg
      361 cgaatacctg ctcgccgaag cggtcatcga gcgccttgcg cgcgtcgtcg atccggcggc
      421 gttgcgcgcc atgctgagcg gcgtgcgcct cgatctcgcg agcgagtcga ccgcgcggcg
      481 cagcgcgtcg gcgctgcagg ccgcgatcga cgacaccgaa gtcgccgccg aagcgcgtta
      541 cgacgccacc aacgaggtgc atcgcatcat catcgtgcgc acgcatcacg gcacgccgca
      601 cgcgacggcg atcgacgccg atttcctcgc cagcggcgac gccgaccaga tccgtcgcgc
      661 tgccgacgtg ctccaggacc tcatctatcc cggcgcggtg gtcaagcgcg gcgacaagca
      721 gcaggcggtg aaatcattca gggaagcgct cgactggctc ctcggcgagg cacgcggcag
      781 cgtcgcgatc cagcgctaca agggcctcgg cgaaatgaac ccggggcagt tgtgggagac
      841 cacgatggac cccgcggtgc ggcgcctgct caaggtgcag atcgaggacg cgatcggctc
      901 cgaggagatc ttctcgacgc tgatgggcga gcaggtggaa ccgcgccgcg cgttcatcga
      961 gagcaacgcg ctcggcgtgc gcaacctgga cgtctgaaaa ggcgcagcat gatgtaggga
     1021 ggtcggatgc gcgcgtggtt cggttcaccg cttcgcggct tcgccgcgct gctcgtcacg
     1081 ctgatcgcgg ccggcgtcgc acaggcggcg ccgccttcgg tcgaagcatt cttccgcaag
     1141 cccgcgtacg gcggtgcgac gctgtcgccg aacgggcgct atctcgccgt cgtcgcgccc
     1201 gtcggcgagc ggcgcgggct gatcgtgctc gacctcgagg cgaaagccgc gacccgcatg
     1261 caatcgcccg gcgacggcga tgtgctcggc gccgtctggc agaacgacaa gcggctggtc
     1321 gtgcgcatcg gcgagttgca gcgcgcaagt ggcgagccgc cgcgcgaagc gggcctcgtc
     1381 gcgatcgacc gcgacggcac cgactcgcgg gtggtcgccg gcatccagcc acgcaccatg
     1441 gccggctcga gcatcgtcga gaaacgcttc gagcggccgt ggttcgtgcg gctgttgcgc
     1501 gtcgtcgagg gcacgaacga cgtgctgctg acggcgcgcg agcgcaatgc cgagacgctc
     1561 gacgtctacc gctacgacac ggtgagcggc gagaagacgc tgctcacgtt cgattcgccg
     1621 ggtgccgtgc agcattgggt cgtcgatttc gacggcgttc cgcgcgccgc gctcacggcc
     1681 gatatcgacc atgacgtttc ggcgtggtac gtccgcaagt cggcgggcga tccgtggatc
     1741 gaggtcgagg cggcgaagct cggcgcgctg aactccgtcc cgctcgaatt cgatcccaac
     1801 ggcaagctgc tctacgtgtc ggcgcgcagg aacggcgacg atcgcgcgtc gatctacgaa
     1861 tatgcgatcg acgcgcatgc atggcgcaag ccgatcgtgc agcatccgct gcgcgacgtc
     1921 gatggcggca acgcatcgtt tcgaagcgat tacagggcgc gcgagattct cgggctcgca
     1981 tacaccgacg acaagccgtc ggtggcgtgg ttcgacgccg ggtatgcgcg cgtgcagaaa
     2041 agcgtcgacg ccacgctgcc cgacaccgtc aacctgctgc agcggcgagg cgatcgtttt
     2101 ctcgtcgtcg cattctccga tcgcgatcct ggcgaagcgt atctgctcga cacgaagtcg
     2161 atgcggctcg aaaagctctt ttcctacgag ccgtggatcg atgtgagcgc gatggctgcg
     2221 cagcgctggg ttcgctatcc cgcacgcgac ggcctgacga tccccgcgct tttgaccgtc
     2281 cccccgaatg cccgcgtgaa gccgttcccg ctcgtcgtcg tgatccatgg cggaccgaac
     2341 gtcgaggcga cgcgctgggg ttacgacgcc gagacccagt ttctcgtttc gcgcggctac
     2401 gccgtgctgc aaccgcagtt ccgcggcacc ctcggcttcg gctggaagct tcgctcggcg
     2461 ggctttcgca aatggggcga cgagatgcag gacgatctcg aggacggcgt caaatgggcg
     2521 atcgcgcagg gcatcgccga tcccgggcgc gtctgcttct acggcgcgag ctacggcggc
     2581 tacgccgcgg cgtggggtgc gatcaagaat gccaagctca tcaagtgcgc ggtgtcgtat
     2641 gtcggcgtga tgtcgatcga ttacctgttc gacaacgcgc agaccgacct ttcgcgtctc
     2701 gccgaccgat cgtcgctgat ggtgcgcgag atcggcgacc cgaaaatcga gcgcgcgcgc
     2761 tttcgccgcg tcaatccgat cgacaacgcc gacaaggtcg gcgtaccgat cctgctcgcc
     2821 tacggcgcct cggaccggcg cgtgccgctc gtccacggca gcgactttcg caacgccctc
     2881 gaccgcgcgc acaagccgta cgagtgggtc gtctatgccg acgaaggaca cggtttcgtc
     2941 aaggacggga atctgttcga cttctatggc cgcgtcgacc gcttcctcgc gaagtatctc
     3001 gacgcatccg ctgcgcctgc ggcaactgca gcgaccgtcg gcaggtgatc gacgtgcacc
     3061 gcggagctca cccggatacg aatcgaatgc ccgcctcccc tgtgcgcacg ctcgccgtcg
     3121 cggcgatgct gactctcgcc acgctggctc acgccggcgg acccacgcgg ctcatcgtca
     3181 agctgaagaa cgccgccgcc aaggcggccc ccacgccgca ggcgcgcatc gagaagcttt
     3241 cggccgccag cggcctgcgg ctcgcccatg tacgggcgat ggcgctcggc gccgacgtcg
     3301 tcatcgcgcc cgatgccgac acgcgcagtg cgctcgcagc gattgccgcc gatcgtgacg
     3361 tcgaattcgt cgaggtcgac gcgcgtgtgc acgcgatgca ggcggcgccg ccggtcaacg
     3421 acactttcgc gccccagcag cgctatcttg ccgacacgcc gacagcgatc agcgcctacg
     3481 acgcgtggaa cgtgacgcac ggctcgccga acatcgtcgt tgccgtcgtc gattcgggat
     3541 atcgtccgca cgccggcatc gccgggcgct ttcttcccgg gtacgacatg atttcggaag
     3601 ccgacgtcgc caacgacggc gacggccgcg atgccgatgc atcggatccc ggcgactggg
     3661 tgctcgcaag cgaggcgagc ggcgactgcc acgcgcacga cagcgtctgg catggcacca
     3721 gcgtcgccgg catcatcggt gcgaacacca acgacggctc gtggaccgcc ggcatcgact
     3781 ggaacgcccg cattctgccg gtccgcgtgc tgggcaagtg cgggggcttc gattcggacg
     3841 tcgtcgacgg catcgcgtgg gccgccggcc tttcggttcc cggcgtaccg ccgaatccga
     3901 cgcccgcgca tgtaatcaac ctgagcctcg gctcgtcgcg cgcatgctcg gcggtgtaca
     3961 ccgatgtcgc ggccgccgcc tatgcgcacg gcgtgacgcg cgcgatcgtc gcggccgccg
     4021 gaaacgactc gcaggaggtc tccaacgaca accccgcgag ctgccccggc tattacgcca
     4081 tcgcgtcgac gacgggcgtc agtggcaagc tcgcgtcgtt cagcaacttc ggccccggaa
     4141 tcacgttgtc ggcgccgggt ggcgcggcga atttccgcac ccctgccgac tcggcgctcg
     4201 tcctttcgaa ttccggcacg acgacgccgt tggccgacat cgtcgtcagc gaagggggca
     4261 ccagcttctc ggcgccgatg gtttcggcga cggtgtcgtt gatgctcgcc gtggcgccgt
     4321 tcctcacgcc cgggcaggtg cacgacatca tcgttggcac cgcaaaggcg tttccgtcga
     4381 catccgactg cacgaccgac cgctgcggag cgggaatcgt cgacgtcggc gccgccgtgc
     4441 gcgcggccgc gagtgcatcg ccgccgccgg cggcggtcaa ctaccagggg ctgtggtggg
     4501 cgacgccacc gggatccgaa tcgggttggg gcatcaactt cgcgcatcag ggcgacgtca
     4561 tcttcgcgac gtggttcaca tacgacgcca ccggcaaggc gtggtggctg tcgatgaccg
     4621 caagcaggag cgggcagaac gtttattcgg gcgacctgct gcaaacgcac ggccccgcgt
     4681 tctcggcgac gccgttcgat ccgaacgccg tgacgcacag cacggtgggc catgcgacgc
     4741 tcacgttcac cgatgcgaac accgcgcaat tcgtctacac ggtgaacggc atcgcacaga
     4801 cgaagacgct cacgcgcgag ctcttcggta cggcgcccgc ctgcaacttc ggggcacagc
     4861 cgaacctggc acttgccacc aactaccagg acctgtggtg ggctgcgccg gcgggcgtcg
     4921 aatcgggctg ggggatcaac ctgaccgagc agagcagcat gatcttcggc acgtggttca
     4981 cctacgacgt cgacggcagc ccgctgtggc tttcggtgct cgcgacgaac accgcccccg
     5041 gcgtgttcac cggcgacctg gtgcgcaccc gcgggccgcc gttcagcgca acgccgttcg
     5101 atcccgcgat ggtgacgcac accggcgtcg gcaccgcgac gttcacgttc agcgacggga
     5161 gccacgcgac gttcgcgtac accgtgggcg gcgtgacgca gtcgaagccg atcacgcgcg
     5221 aagtgttcgt gaaccccggg accttttgcc aatgactcgc caatcatgac cgggcagcgt
     5281 ccccgaggtc gcgatcctcg cgtcacggcg gtgctcgaac gtcttggcct gaccgtcgac
     5341 gacgacgata ccgtgcatcg gccgcgggtg cctgtcgccg acggctcccg gctcgtatcg
     5401 ttcaacccgg cgaacgagca ggcgcttggc gcgattgcga caacgagtgc ggccggcttc
     5461 gacgtgctgc tttcgagcgc cgtcgacgcg tcgctcgcct ggcgcgatac cccggcaccg
     5521 gtgcgcggca atgcggtgcg ccgttacgcg cagttgctgc gcgagcacaa ggatgcgctc
     5581 ggaacgctcg tttcactcga aacgggcaag atcaaggccg agggcgatgg cgaagtccag
     5641 gagatgatcg acatcgccga cttcgcggtc ggccagtcgc ggatgctgta tggcaagacg
     5701 ctgcattccg agcgcccgtc gcaccggatg tacgagcagt ggcatccgct gggcgtggtg
     5761 ggcgtgatca ccgcgttcaa ttttcccgtt gccgtatggg cgtggaacgc gatgcttgcg
     5821 tgcgtttgcg gcaatgcggt cgtgtggaag ccgtcgccga aaacgccgct cgcggcgctt
     5881 gccgcggcgc atctcgccga ccgtgtcgtc gacgagcagc atctgccgca gatcttctcg
     5941 ctcgctctcg gggacgacgc gctcggccgc gcgctcgttg ccgacccgcg ggttgcgctc
     6001 gtgtcgttca ccggctcgtc acgtgtggga caggcagtcg ccggtgcagt gagcgcccgc
     6061 ttcggcaaag tgctgctcga atgcggcggc aacaatgcgg tgatcgtcgc cgacgatgcc
     6121 gatgccgatc tcgtcattcc ggccgtcgtg ttcggcgccg tgggtacggc gggccagcgc
     6181 tgcacgacga cgcggcgatt gatcgtccat cgctcgcggg tagacgatgt cgtgactcgg
     6241 cttgccaacg cgtatgcgca ggtgcggatc ggcgatccgc tgcaaccgtc gacgctgatg
     6301 ggcccgctga tcgaccgcgg cgccgtcgac ggttaccttc gcgcgatcga cgaggcgcgt
     6361 gcagccggcg caacgatcgt gtgcgggggg cgcatcgtgc cgggcgacgg gtttttcgtc
     6421 gaaccgacga tcatcggcgg actcgacagc gcgcatcccc tggtgcagcg cgagacgttc
     6481 gcgcccatcc tgtacgtgct ggcgttcgat acgatcgacg atgcgatcgc gcgcaacaat
     6541 ggcgtgcgcc aggggctctc atcggcgctg ttcacgcaga gcctgcgcac cgccgaagcg
     6601 ttcctggcgg cgtcgggcag cgactgcggc attgcgaaca tcaacatcgg cacgtcgggc
     6661 gccgagatcg gcggcgcgtt cggcggcgaa aaggaaaccg gcggcggacg cgaatcggga
     6721 tcggacgcgt ggcgcgccta catgcgccgc cagacgaaca cgatcaactg gagccgcgag
     6781 cttccactcg cacagggaat ccgcttcgag atcgatcgtc cggcgccgta gatcggcgcc
     6841 gtagatcggg gtcagagctg taattcgcgc accataggtg gcattcaacc gcagcatgcg
     6901 ccgcgaatta cagctctgac cccgatctcg cagcatgcgc cgcgaattac agctctgacc
     6961 ccgatctcgt tacttccgca acagccagac aacaagcgtg agcaccagcg acacgagcac
     7021 gccggtcgtc agcggaaaat ggaacgacca gccgtcgcgc tcgatgttga tgtcgcccgg
     7081 caagcggccg agcccgagct tcgataccca cggccatgcg acgccgacga gcagcatcac
     7141 gacgcccagc acgatcagca gacgttgcat cgggaggcgc gatgacgggt agccggagcg
     7201 gcgactagcg tcgcttgctc ttccttgccg tcgcgctggt gcctgtcttg cgtgcgctcg
     7261 gcgccttcgc gcgcgtggtt ctcgacgtgg ccgtgccctt gcgcgtgacc gtggtaccgc
     7321 cgccggtttt tgcgtccggt gcgatgcgct tgaccgcagc gcgcttcttc accacgctcg
     7381 cgcgtgaagc ggctgccttc gctgcgggtg cgccaacggg cttcgccgcg ggcgcacgct
     7441 tgtctgccgg cgcggttcgc gtcgacgcgc gcttggcagc cggcgccacc gcgtcctcgg
     7501 aacggccgcc gcgcgcgcct gcgatgcgcc gcaccgcttt gaccggctcg gccggcgtgg
     7561 ctgcgcgggg cttgcgcggc gcgcgtgtct tcgcaccggt tcgcgtggcg gctcgcggct
     7621 cgcgtccggc cttttcgtcg accagcgcga tggcctgctc gagcgacagc gaatcgacgg
     7681 cgtcgcggtc gggcagcgtc gcgttcgtgg cgccatgctt gacgtacgga ccgtaccgcc
     7741 ccgcgtgcag ctcgacgggc tggcgatcgc gcgggtgcgc gccgagcacg cgcaacgggg
     7801 cgctgcgcat cggtcgcgcg ccgcgccgcc ccggcgcttt cgcctcgaac tcgaagccga
     7861 ccttgccatc ggcctgcttg acgagatacg ccgagaacgg acgccgcgta cgcgcggaca
     7921 cgaattgcag aagatcggtc ttgccggttt cgagcagctt cgtcatctgc acgggctcga
     7981 tcgtcttctg gaggatcgtg cggcccgagc ggaaatcgca actgcggtct tccccgacgg
     8041 agcgttcgca cacgtacgcg ttcggcgtct cgaacacgcg attgccgcac ttcgggcagg
     8101 ggccgagcgg agccagcgac gaaaaatcgt agcttccccc ttcctcgtcg cccggacgcg
     8161 gtccgaaatc gaactccacc tcgttggcgt cgttgagctt gagcttcgcc gagaacggac
     8221 gcccgatgcg gctgcgaaag ccgtcgagtg gaccggcgct gcgctcggtg atcagcgtgt
     8281 cggcctcgga cggctcgatc tggcggccgc ccatgatctt ccagaagccg aaatcgcaat
     8341 tcacgcaggc gaacttcttg tactgctcgt gcacttcgcc gccgcatttc gggcagcgcg
     8401 cggaaagcgt cgcgaagtcg ccggggaccg tgtcgctttc gtagtttttc gcctgcgcga
     8461 cgatgtgctt cgtcatgccg acgatctcgt gcatgaactc gcttcgcgcg agcgcgccgt
     8521 gctccatttg cgcgagcttg aattcccatt cggccgtgag ctcgggcgag aagagctcgg
     8581 gcacgccgag gccgtgcagc agcgtgatca gcgaagacgc cttcggcgtg gcgatcagct
     8641 ccttgccctc gcgcagcacg tagcgctcgg cgatcagccc ctcgatgatc tgcgcgcgcg
     8701 tcgccggcgt gccgaggccc ttctcgcgca tcgcttcgcg cagctcgtcg tcctcgatcg
     8761 acttgcccgc gccttccatg gccgacaaca gcgtcgcttc gttgaagcgc ggcggcgggc
     8821 gggtggtcag tgcgacgacg tcgacgcttc gcgtgtcgac tttctcggtg ccctcgtagc
     8881 gccgcacggc ggttgcgcgc gcatcgggcg ccgccttcgc gtcgacgatc tcgcgaacct
     8941 tcacgggcgt gagcgtcggc tcgtcgccgg ccgcttcctt gccgtacacc gccagccagc
     9001 cgggctggac cagcaccttg ccttcggact tgaacggctc gccgg
//
LOCUS       DASSVX010000200        16504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1641264, whole genome shotgun sequence.
ACCESSION   DASSVX010000200 DASSVX010000000
VERSION     DASSVX010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 16504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16504
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641264"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1266)
                     /locus_tag="VFO53_08405"
     CDS             complement(211..1266)
                     /locus_tag="VFO53_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET8876335.1"
                     /translation="MPETTAAPAHVPLAEVLRGRAVESVHFGSVAVVDGSGNVLRAAG
                     DPFTLTFTRSALKPLQALAFVAGGGAARFGFSDAQVALMCASHSGEPRHVDAVADMLA
                     RCGCSSGDLQCGTHAPGFYDARGEVPPPPPYSTLSHNCSGKHSGMLAYCVQHGQPIGD
                     YLAFDHPLQQAIRRAVSKFTAVAEERLVAGIDGCSAPNYAVPLASLATAFARLAAADV
                     DPDYGRAPRTLANAMIAHPEMVSGELRSDLALMQAGRGDWVTKIGAEGVQAIGIRSQG
                     LGIAIKVADGQKRGLYPAIVAVLDQLGVVDAVARAALERWRIGTIRNYRGTVTGEIRP
                     AVELAAFRPQLSAFTDA"
     gene            complement(1345..3279)
                     /locus_tag="VFO53_08410"
     CDS             complement(1345..3279)
                     /locus_tag="VFO53_08410"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HET8876336.1"
                     /translation="MSRSEDCAMTSYADFHRRSLDDPDAFWRDEARLIDWHTPFAQVL
                     DQSRPPFAKWFVGGRTNLCHNAVDRHVAARGEQPALIYISTETGHSQTYSYAQLFDEV
                     NACAAMLKAQGVGRADRVLIYMPMIAEAVFAMLACTRIGAIHSVVFGGFAATSLATRI
                     DDARPALLVTADAGMRAGKAIAYKPLVDEALRIARHPPRRVIVVDRGIDRGFARTPGR
                     DLDYAQLRSEHDGARVPCEWLESSEPSYILFTSGTTGQPKGVQRDTGGYAVALASSMR
                     RIFCLEPGETMFTTSDIGWVVGHSYIVYGPLINGSPTIMYEGLPIQPDPGIWWKIVED
                     NRVKTMFSSPTAARVLKKHDPAYIRKHDLGSLNYLFLAGEPLDEPTARWASEALGVAI
                     VDNYWQTESGWPILAAQLGVEETPRKFGSPSFPVYGYDVKLVDGVTGADVGTGQKGVL
                     AIAPPLPPGCMTTVWGNDERFVSTYFSTVPGRDLYSTFDWATRDEDGYYFVLGRTDDV
                     INVAGHRIGTREIEEAVQAHAGIAEVAVVGVHDEVKGQVPIAFAVVKDAARIASAAAT
                     ESMAAEVMQTVDRELGAIARPARVHFVTLLPKTRSGKLLRRSIQALAESRDPGDLTTI
                     EDPAALEQIRAALAAARPAR"
     gene            complement(3326..4330)
                     /locus_tag="VFO53_08415"
     CDS             complement(3326..4330)
                     /locus_tag="VFO53_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HET8876337.1"
                     /translation="MQTFKAYRTFEQDKVVSSRFVTMTQDELDRGEVLVRTKYSTINY
                     KDALSYNGSGKIMRKFPTVAGIDLAGTVESSSDPRWKRGDKVIVTGYDLGVAHDGGYA
                     EYARVPADWVVRRPESMTAFDAMTLGTAGFTAALAIHLMQHNGLVPDAGPVAVTGATG
                     GVGSVAIEILSKLGYRTVAITGKGRETDYLRDIGASDVMLRSSLDFSKIRPLEKATWA
                     GAVDNLGGDMLSWLLATSKIGGTVAAVGLAADMKLDTTVAPFILRGVHLLGTDSANTP
                     MSLRQTLWNRLAVEWRPDRVHDKVSTIDFDELPMHFDAYLKGMARGRTVVRVAADSHP
                     "
     gene            complement(4369..5514)
                     /gene="zapE"
                     /locus_tag="VFO53_08420"
     CDS             complement(4369..5514)
                     /gene="zapE"
                     /locus_tag="VFO53_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HET8876338.1"
                     /translation="MIGAEDPGQISRRHGGLIDFLEPLLDQRRSTLDHAQIGALDRLQ
                     QLADDLAAFSAARKSALKRLFGGPGVPRGVYLWGGVGRGKSLLMDSFYATVGIRRKAR
                     VHFHAFMRDVHAALALLSDEADPLARVAAQIANRYRLICLDEFHVDDIADAMILGRLV
                     EGLFANGVVLVVTSNYAPDDLWPNGLLRERFLPAIERIKASLDVVEVDAGVDYRLRAL
                     AHVETFHVPAGAAADAAMTAAFEAMRSGPDESTHLTIDGRTLAARRRAGSIVWFDFGS
                     LCDGPRSQRDYLDVARGFSVLFLSDIPRMGADAADRARRFTWLIDILYDHRVKLVASA
                     AVSPGALYAEGPNVRDFSRTVSRIAEMRTRDYMALPHITDGAGARAA"
     gene            complement(5540..6973)
                     /gene="lpdA"
                     /locus_tag="VFO53_08425"
     CDS             complement(5540..6973)
                     /gene="lpdA"
                     /locus_tag="VFO53_08425"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET8876339.1"
                     /translation="MADAQQFDVVVIGGGPGGYVAAIRAAQLGFATACIDDWTRDGGK
                     PALGGTCTNVGCIPSKALLQSSENYAQAGHAFAEHGIEIGALSMHVGTMLARKDKVVG
                     QNNDGIAYLFRKNKVAFFHGRGAFAGSDAGRWTITVDGPGAATLAARNVIVATGSAPR
                     ALPGVAFDNARVLDNAGALAIPDVPKRLGVVGAGVIGLEMGSVWRRLGADVTILEALP
                     VFLGAADEAVAREAQKQFAKQGIAIRLGVTIGAVRTGSGEVTVDYTDASGKAQSATFD
                     RLIVSIGRVPFTSGLGADKVGLELDARGFVTVDAECRTNLANVFAIGDVVRGPMLAHK
                     AEEEGVAVAERIAGQHGHVDFNAIPWVIYTSPEIAWVGKTEQQLRSDGVAYRAGSFPF
                     SANGRARALGDTVGFVKIVADAKTDRVLGVHIIGPWASELIAEGVMAMEFGASSEDIA
                     RICHAHPTLSEATREAALAVDKRTLNL"
     gene            complement(6973..8271)
                     /gene="odhB"
                     /locus_tag="VFO53_08430"
     CDS             complement(6973..8271)
                     /gene="odhB"
                     /locus_tag="VFO53_08430"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HET8876340.1"
                     /translation="MRIEVKVPQLPESVAEATLVNWHKKPGEAVRRDENLIDIETDKV
                     VLELPAPNDGVLAEIRQPDGATVTSNDVIAVIDTDTKADTGAAQHAASASTPAATQPA
                     ASAKPPSAASTAPETAATVTALPSARKMMAEQGVDPSSVAGTGRGGRITKGDVADALG
                     TRASAPATPPTPTAKAPPAAPAAPAAKAPPSAPPAVTQGARPEQRVPMSRLRARIAER
                     LVESQSTAAILTTFNEVNMQAVIELRNRYKERFEKEHGVKLGFMGFFVKAAVHALKKF
                     PLVNASIDGADIVYHGYYDIGIAVGGPRGLVVPILRDADQMSIADIEKKIGDFGRRAQ
                     EAKLTVEELTGGTYTISNGGVFGSMLSTPIINPPQSAILGVHATRERPVVENGQVVVR
                     PVNYLAQSYDHRIIDGREAVLSLVAIKEALEDPARMLLDL"
     gene            complement(8293..11136)
                     /locus_tag="VFO53_08435"
     CDS             complement(8293..11136)
                     /locus_tag="VFO53_08435"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET8876341.1"
                     /translation="MMGSMQELEASSTLFGSNAPFIEELYESYLADPANVSPEWRSYF
                     DAIKGDAADVPHASVVESFRELARSRRVAGAMVDETTMHKQVLVLRLISKYRTLGMFH
                     ADLDPLSRQEPAYIPDLDLRTYGFTDADLDTPFDVGSFKAGPARMRLRDIIAALEETY
                     TRTFGAEYMYISDTPTKRFVQERLEPIRSKPKFSPEERRHILERLTAAETLERYLHTK
                     YVGQKRFSGEGGDSMIPMLDLLIQRAGAAGVGEIVLGMAHRGRLNVLVNTLGKMPSDL
                     FSEFEGKVAQDLPAGDVKYHQGFSSDVSTPGGPVHVTLAFNPSHLEIVNPVVEGSVRA
                     RQHRRKDARGDQVLPVLLHGDAAIAGQGVVQECLNMAHTRGYYTGGTIHIVINNQIGF
                     TTSDPRDVRGTLYCTDVAKMVEAPIFHVNGDDPEACLLATELALDYRQKFHKDVFVDL
                     VCFRRLGHNEADEPMITQPLMYKKINQHPGTRKLYAERLIAEGVIGADDADTMIATYR
                     AAMDKGYHTNTTVLSNYKPPFIVDWSPYKNRRWTDPADTSVPLARLTELGIKATTVPS
                     NFKLHPRVEKIMVDRRAMVEGKLPVDWGMGEILSYATLLDQNFGVRMSGEDIGRGTFS
                     HRHAVLHDQNREKWDLGTWIPLQHVKEGQPDFEIIDSVLTENAVLAFEYGYTTSEPTQ
                     LVIWEAQFGDFANGAQVVIDQFISAGEVKWGRICDLVLFLPHGYEGQGPEHSSARPER
                     YLQLCAQHNMQVVVPTTPAQIFHLLRRQMIRQFRKPLIVITPKSLLRHKEAVSSFDEL
                     ASGAFRNVIGESDAIDPAKVRRVIVCSGKVYFELRAYRREQKIGDVAILRLEQQYPFP
                     HDEYKREVGRYKAAKEVVWCQEEPQNQGAWYRLNSYLRADIDANKVLAYAGRPVSASP
                     AVGYAAKHNAEQKQLIEDAFAATLRSGGMLLAR"
     gene            complement(11214..12521)
                     /locus_tag="VFO53_08440"
     CDS             complement(11214..12521)
                     /locus_tag="VFO53_08440"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET8876342.1"
                     /translation="MTDSTATLQFSDGSPSVTFPILTGTIGPQVIDIRTLYAKTGKFT
                     YDPGFMSTAACSSAITYIDGDNGELLYRGYPIEELAVRTDYLGTCYLLLYGELPNRAQ
                     KDDFVSRVTNHTMVNEQMQFFMRGFRRDAHPMAVLTGLVGAMSAFYPDSINLNDAHQR
                     DISAIRLIAKMPTLVAMAYKYSCGQPYMYPRNDLPYAGNFMRMMFATPCEDYVVNDVL
                     VRALDRIFILHADHEQNASTSTVRLCASSGTNPFAAIAAGVACLWGPAHGGANEAALN
                     MLYDIQAQGGVAKIGEFIAQVKDKHSNVRLMGFGHRVYKHYDPRAKLMRETCYEVLGD
                     LGLGDDPLFKLAMALEKIALEDDYFVQRKLYPNVDFYSGIVQKAIGIPVQLFTAIFAL
                     ARTVGWIAQLNEMVSDPEYKIGRPRQLFTGAARRTVKPISERG"
     gene            complement(12550..12795)
                     /locus_tag="VFO53_08445"
     CDS             complement(12550..12795)
                     /locus_tag="VFO53_08445"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HET8876343.1"
                     /translation="MVIDNARLRRIRWRCRRGMLENDLILSRFLDRCADTLTEDDIAN
                     LDALLHMSDNALWDLLSGRAEADDPRLASFAARLAQG"
     gene            complement(12889..13584)
                     /locus_tag="VFO53_08450"
     CDS             complement(12889..13584)
                     /locus_tag="VFO53_08450"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HET8876344.1"
                     /translation="MKFRIYRYDPEKDAKPRLQEYDIALDPHDRMLLDALIRLKATDD
                     SLAFRRSCREGVCGSDAMNINGKNGLACVTNLKNLKEPVELRPLPGLPVIRDLIVDMS
                     QFFKQYHSVRPYLVNDTPPPERERLQSPAAREQLDGLYECILCACCSTACPSFWWNPD
                     KFVGPAGLLQAYRFIADSRDHDVNQRLDNLEDPYRLFRCHTIMNCVDVCPKNLNPTLA
                     IEKIKDLMVKRTV"
     gene            complement(13601..15364)
                     /gene="sdhA"
                     /locus_tag="VFO53_08455"
     CDS             complement(13601..15364)
                     /gene="sdhA"
                     /locus_tag="VFO53_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HET8876345.1"
                     /translation="MKLPTRRFDALIVGAGGAGMRASLQLAAAGLTVAVLSKVFPTRS
                     HTVAAQGGIGASLGNMSEDSWLWHMYDTVKGSDWLGDQDAIEYMCRMAPQVVYELEHF
                     GMPFDRNPDGTIYQRPFGGHSMNFGEKSVRRACASADRTGHSMLHTLYQQNVRAHTQF
                     FVEWMALDLIRDSDGDVVGVTALDMETGDVVALEAKVTVLATGGAGRIFAASTNAFIN
                     TGDGLGMAARAGVPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNCNGERFMERY
                     APNLKDLASRDIVSRSMDQEIKEGRGCGPHKDYVLLKLDHLGPDVIMKRLPSIREIAI
                     KFANADPIKEPIPVVPTIHYMMGGIPTNIHGQVVAPRHGDPGVVVEGLYAVGECACVS
                     VHGANRLGTNSLLDLLVFGRAAGDFIVSRNLAALAHKALPPDAGDASLARLARLDAAT
                     SGERVADVGNELRKTMQSHCGVFRNEALLAEGVAKVMELENRVARSAIDDKSRTFNTA
                     RVEALELDNLIEVAKATIVSALARHESRGAQARSDYPNRDDVHWLKHTLWYREGNRLD
                     YKPVHLTPLSVESFPPKARTY"
     gene            complement(15390..15749)
                     /gene="sdhD"
                     /locus_tag="VFO53_08460"
     CDS             complement(15390..15749)
                     /gene="sdhD"
                     /locus_tag="VFO53_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HET8876346.1"
                     /translation="MVAGTDKLPVGAHYGLRDWLSQRVTAIVITLYTALVLGIVLWHG
                     GLDYPTARALFAGNAFRVATFLFMASLFWHAWVGVRNILMDYAKPYGVRLMLEIVVVC
                     LLVAYGGWTIGVLWGNP"
     gene            complement(15743..16141)
                     /gene="sdhC"
                     /locus_tag="VFO53_08465"
     CDS             complement(15743..16141)
                     /gene="sdhC"
                     /locus_tag="VFO53_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HET8876347.1"
                     /translation="MAAADRPVDKPRPVYLNLFAIRQPLPAIASILHRISGALLFIAG
                     IPLLLWDVQRSLASPEGFAAATAPLGTPFGKLVFLVLAWCFLFHLLAGLRHLALDLHI
                     GIGLAPARGSAALVLVLSILLALIVAVRLW"
     gene            complement(16357..>16504)
                     /locus_tag="VFO53_08470"
     CDS             complement(16357..>16504)
                     /locus_tag="VFO53_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HET8876348.1"
                     /translation="ATAALLRVAAGAPLLAVERVAYTYGDRPVEVRRGLCTTRHEHYW
                     NALT"
BASE COUNT         2635 a         5523 c         5586 g         2760 t
ORIGIN      
        1 cacctcgttg cgcgcgcagc acgagctcgt gatcgacttc gcgatcaccc atccgccggc
       61 agtccctgat tcgcgtcgtt gttatgcccg ccgaacggcg ggtgcggctg cctgcgatgc
      121 cgagcggctg tcccggggtg ggaggcgcga aatggcacgt ggcgcacgct tggagcaaca
      181 gaccgttgcg cgcagcccaa gttcagccga tcacgcatcg gtaaacgcgg ataattgcgg
      241 acggaaggcg gcaagctcaa ccgcggggcg aatttccccg gtcaccgtgc cccggtagtt
      301 gcggatcgtg ccgatgcgcc accgctcgag ggccgccctt gcaacggcat cgaccacgcc
      361 cagctggtcc agcacggcga cgattgccgg gtagaggccc cgcttctgcc cgtcggcgac
      421 cttgatcgcg atgccgagac cctggcttcg gatgccgatc gcctgcacgc cttcggcgcc
      481 gatcttcgtc acccagtcac cccggccggc ctgcatcagc gcgagatcgc tgcgcaattc
      541 gccggacacc atttcggggt gcgcgatcat ggcgttggcc agcgtgcgtg gcgcccggcc
      601 gtaatcggga tcgacgtcgg cagcggcaag gcgcgcaaat gccgtagcga gcgaggcgag
      661 cggtaccgcg taattgggcg cggagcagcc gtcgatgccg gccaccagcc gctcctcggc
      721 gacagcggtg aatttcgaca ccgcgcggcg gatcgcctgc tgcagcggat ggtcgaacgc
      781 aaggtagtcg ccgatcggct gtccgtgctg cacgcagtac gcgagcatcc cgctgtgctt
      841 gccggaacag ttgtgcgaaa gcgtcgagta cgggggcggc ggcggaactt cgcccctggc
      901 atcgtagaac cccggcgcgt gcgtgccgca ttggaggtcg cccgacgaac agccgcaacg
      961 cgccagcatg tcggccaccg cgtcgacgtg ccgcggctcg cccgagtggc tcgcgcacat
     1021 cagggcgacc tgcgcgtcac tgaagccgaa gcgcgccgcg ccgccgcctg caacgaacgc
     1081 aagcgcctgc aacggcttca gcgcgcttcg cgtgaatgtc agcgtgaagg ggtcgcccgc
     1141 cgcccgcagc acgttccccg aaccgtcgac gacggccacc gagccgaaat ggacgctctc
     1201 caccgcgcga ccgcgcagca cctccgcgag cggaacgtgg gcaggggcgg cggtggtttc
     1261 gggcattcgg gcgatcgcgg gcaaaccgcc attatcgcct atcgacggcg gccgccggcg
     1321 cgccgcgata cgcgaggccc gcgatcagcg cgcaggccgc gccgctgcga gcgcggcgcg
     1381 gatctgctcg agcgccgcgg gatcctcgat cgtcgtgaga tccccggggt cgcgcgactc
     1441 ggccagcgcc tggatcgagc ggcgcaggag cttccccgac cgcgtcttcg gcagcagcgt
     1501 cacgaagtgc acgcgcgcgg gccgcgcgat ggcgccgagc tcccggtcga ccgtctgcat
     1561 cacctcggcc gccatgcttt cggtggccgc ggccgaggcg attcgcgccg catccttgac
     1621 cacggcaaag gcgatcggca cctggccctt gacctcgtcg tgcacgccga cgaccgccac
     1681 ttcggcgatg ccggcatgcg cctgtaccgc ctcctcgatc tcccgcgtgc cgatccggtg
     1741 cccggcgaca ttgatcacgt cgtcggtgcg cccgagaacg aaatagtagc cgtcctcgtc
     1801 gcgggtcgcc cagtcgaacg tcgagtagag gtcgcgtccc ggcacggtgg aaaaatacgt
     1861 cgacacgaag cgctcgtcgt tgccccacac ggtggtcatg cagccggggg gcagcggcgg
     1921 cgcaatcgcg agcacgccct tctgtcccgt tccgacatcg gcgccggtga cgccatcgac
     1981 gagcttcaca tcgtagccgt acaccggaaa cgacggcgag ccgaacttgc gcggcgtctc
     2041 ctcgacgccg agttgcgcgg cgaggatggg ccaccccgac tcggtctgcc agtagttgtc
     2101 gacgatcgcg acgccgagcg cctcgctcgc ccaccgcgcc gtcggctcgt cgagcggctc
     2161 gccggcgagg aacaggtagt tgaggctgcc gagatcgtgc ttgcgaatgt acgcgggatc
     2221 gtgcttcttc agcacgcgcg cggcggtggg cgagctgaac atcgtcttga cccgattgtc
     2281 ctcgacgatc ttccaccaga tgcccggatc gggctggatc ggcagcccct cgtacatgat
     2341 cgtcggtgag ccgttgatga gcggcccata gacgatgtac gagtgcccga cgacccagcc
     2401 gatgtcgctc gtcgtgaaca tcgtctcgcc gggctcgagg cagaagatcc tgcgcatcga
     2461 cgacgcgagc gcgaccgcgt agccgccggt gtcgcgctgc acgcccttcg gctggcccgt
     2521 ggtgcccgag gtgaacagga tgtacgacgg ctcggacgat tcgagccact cgcacggcac
     2581 gcgtgcgccg tcgtgctcgg accgcaattg cgcgtaatcg agatcgcgac cgggcgtgcg
     2641 ggcgaatccg cggtcgatcc cgcggtcgac gacgatcacc ctgcgcggcg gatggcgtgc
     2701 gatgcgcagc gcttcgtcga cgagcggctt gtaggcgatg gccttgccgg cgcgcatgcc
     2761 cgcgtcggca gtgacgagca gcgcgggccg cgcgtcgtcg atgcgcgtgg cgaggctggt
     2821 cgccgcgaag ccgccgaaga cgaccgaatg gatcgcgccg atgcgcgtgc aggccagcat
     2881 tgcgaacacc gcttcggcaa tcatcggcat gtagatcagc acgcgatcgg cgcgcccgac
     2941 gccctgcgcc ttcagcatcg cggcgcacgc gttgacctcg tcgaagagtt gcgcgtacga
     3001 gtacgtctgc gaatgcccgg tctccgtcga gatgtagatc agcgccggct gctcgccgcg
     3061 tgccgcaacg tgccggtcga cggcgttgtg gcacagattg gtccgcccgc cgacgaacca
     3121 cttcgcgaac ggcggccgcg actgatccag gacctgcgca aacggcgtgt gccagtcgat
     3181 gagccgcgcc tcgtcgcgcc agaaggcgtc ggggtcgtcg agggagcggc ggtggaagtc
     3241 ggcgtagctc gtcatcgcgc aatcctcgct tcggctgata cgcgttgcct gatacctgat
     3301 accgatccct gattcctgat ccccgtcagg gatgactgtc cgccgccacc cgcacgacgg
     3361 tgcgcccgcg cgccatgccc ttcaggtagg cgtcgaagtg catcggcagc tcgtcgaagt
     3421 cgatcgtcga caccttgtcg tggacgcggt cgggccgcca ttcgacggcg aggcggttcc
     3481 acagcgtctg gcgcagcgac atcggggtat tggcgctgtc ggtgccgagc aggtgcaccc
     3541 cgcgcaggat gaacggggcg accgtcgtgt cgagcttcat gtctgcggcg agccccacgg
     3601 cggccacggt gcccccgatc ttcgacgtgg cgagcagcca cgacagcatg tcgccgccga
     3661 ggttgtcgac cgcgcccgcc cacgtcgcct tttcgagcgg ccggatcttc gaaaaatcga
     3721 gcgacgagcg cagcatcacg tcgctcgcgc cgatgtcgcg caggtaatcg gtctcgcgcc
     3781 ccttgccggt gatcgcgaca gtccggtagc cgagcttcga caggatctcg atggccaccg
     3841 agccgacgcc gccggtcgcg ccggtgaccg caacggggcc cgcgtcgggg acgaggccgt
     3901 tgtgctgcat caggtgaatg gcgagcgccg cggtgaagcc cgcggtgccg agcgtcatcg
     3961 cgtcgaatgc cgtcatgctt tcgggacggc gcaccaccca gtcggcgggc acgcgcgcgt
     4021 attccgcata accgccgtcg tgcgcgacgc cgaggtcgta gccggtgaca atgaccttgt
     4081 cgccgcgctt ccagcgcggg tcgctgctcg attcgaccgt gccggcgagg tcgatgccgg
     4141 ccaccgtcgg gaacttgcgc atgatcttgc cgctgccgtt gtaggacaac gcatccttgt
     4201 agttgatcgt cgagtacttg gtccggacaa gcacctctcc ccggtcgagc tcgtcctgcg
     4261 tcatggtgac gaaacggctc gacacgacct tgtcctgctc gaacgtgcga tacgccttga
     4321 atgtctgcac cggattctcc tgtcggggcg aaaggaatgc gatgggcgct acgcggctcg
     4381 cgcgcctgcg ccgtcggtga tatgagggag tgccatgtag tcgcgcgtgc gcatttcggc
     4441 aatgcggctg accgtgcgcg aaaagtcgcg gacattcggt ccctcggcgt acagcgcgcc
     4501 gggggaaacg gccgcggagg caacgagctt cacgcgatga tcgtacagga tgtcgatcag
     4561 ccacgtgaag cgccgcgccc gatcggccgc gtcggcgccc atccggggta tgtcggacag
     4621 gaagagcacc gagaagccgc gcgcgacatc gaggtaatcg cgctgcgagc gcggtccgtc
     4681 gcaaagcgag ccgaagtcga accagacgat gctccccgcg cgccggcgcg cggcgagcgt
     4741 gcgtccgtcg atcgtcaggt gcgtcgactc gtcggggccg ctgcgcatcg cctcgaacgc
     4801 cgccgtcatc gccgcgtcgg ccgccgcacc ggccgggacg tggaacgttt cgacatgcgc
     4861 aagggcgcgc aggcgataat cgacgcccgc gtcgacctcg acgacgtcga gcgacgcctt
     4921 gatgcgctcg atcgcgggca ggaagcgctc gcgcagcagg ccgttcggcc acaggtcgtc
     4981 gggcgcgtag ttcgacgtga cgacgagcac gacgccgttt gcgaaaaggc cttccaccag
     5041 acggccgaga atcatcgcgt cggcgatgtc gtcgacgtgg aactcgtcga gacagatcag
     5101 ccgataccgg ttggcgatct gcgcagcgac gcgagccagc ggatcggcct cgtcgctgag
     5161 cagcgcgagc gccgcatgca cgtcgcgcat gaacgcatgg aaatgcacgc gcgccttgcg
     5221 ccggatgccg acggtcgcgt agaagctgtc catcaacagg cttttcccgc gtccgacgcc
     5281 gccccacaga tacacgccgc gcggtacgcc ggggccgccg aacaggcgct tcagtgccga
     5341 ttttcgtgcc gcgctgaacg cggcgagatc gtcggcaagc tgctgcagcc ggtcgagtgc
     5401 gccgatctgc gcatgatcga gcgtcgagcg ccgctggtcg agcagcggtt cgaggaagtc
     5461 gatgagcccg ccgtggcggc gggaaatctg gcccgggtcc tcggccccga tcatgccgac
     5521 gcttcgtctt ccacggccgc tacagattga gcgtgcgctt gtcgaccgcg agtgccgcct
     5581 cgcgcgtggc ttccgacagc gtcggatgcg cgtgacagat gcgcgcgatg tcctcgctcg
     5641 acgcgccgaa ctccatcgcc atcacgccct cggcgatcag ttcgctcgcc cagggaccga
     5701 tgatgtggac gccgaggacg cgatcggtct tggcatcggc gacgattttc acgaagccga
     5761 cggtgtcgcc gagcgcccgc gcacggccgt tcgccgagaa cggaaagctg ccggcgcgat
     5821 aggcgacgcc gtcgctgcgc agctgctgct cggtcttccc gacccaggcg atttccggcg
     5881 acgtgtagat cacccaggga atcgcgttga agtcgacgtg gccgtgctga ccggcgatgc
     5941 gttcggcgac ggcaacgcct tcctcctcgg ccttgtgcgc gagcatgggc ccacgcacga
     6001 cgtcgccgat ggcaaagacg ttcgccagat tggtgcggca ttcggcatcg acggtgacga
     6061 agccgcgcgc gtcgagctca agaccgacct tgtcggcgcc gaggccggac gtgaacggta
     6121 cgcgtccgat cgacacgatc agccggtcga acgtcgcgct ttgcgccttc ccgcttgcgt
     6181 cggtgtagtc gaccgtgact tcgccgctgc cggttcgcac cgcgccgatc gtcacgccga
     6241 gccggattgc gatgccctgc ttcgcgaact gcttttgcgc ttccctggcg acggcctcgt
     6301 cggcggcgcc cagaaacacc ggcaacgcct cgaggatcgt cacgtcggca ccaagacgcc
     6361 gccacacgct gcccatctcg aggccgatga cgccggcgcc gaccacgccg aggcgcttcg
     6421 gcacgtcggg gatggcaagc gcgcccgcat tgtcgagcac tcgcgcattg tcgaacgcga
     6481 caccgggcag cgcgcgcggc gccgatccgg tggcgacgat cacgttgcgc gccgcgagcg
     6541 ttgccgcacc cggtccgtcg accgtgatcg tccagcgccc ggcgtcgctg cccgcgaacg
     6601 cgccgcggcc gtgaaagaac gccaccttgt tcttgcggaa caggtaggcg atgccgtcgt
     6661 tgttctggcc gacgaccttg tccttccgcg caagcatcgt tccaacgtgc atcgacagcg
     6721 cgccgatctc gatgccgtgc tcggcgaatg cgtgtcccgc ctgcgcatag ttttcggacg
     6781 attgcaaaag cgccttcgac gggatgcagc cgacgttcgt gcacgtgccg ccgagcgccg
     6841 gcttgccgcc gtcgcgcgtc cagtcgtcga tgcacgccgt cgcaaagcca agttgcgccg
     6901 cacggatggc ggcgacgtag ccgccggggc cgccgccgat cacgacgacg tcgaattgct
     6961 gcgcgtcggc cattacaggt ccagcaacat ccgagcagga tcctcgagcg cttccttgat
     7021 cgcgaccagc gacagcacgg cctcgcggcc gtcgatgatc cggtggtcgt acgattgcgc
     7081 cagataattg acgggccgca cgacgacctg gccgttctcg accaccggcc gctcgcgcgt
     7141 cgcatgcacg ccgaggatcg ccgattgcgg cggattgatg atcggcgtcg acagcatcga
     7201 tccgaacacc ccgccgttgc tgatcgtgta ggtgccgccg gtcaactcct cgacggtcag
     7261 cttcgcttcc tgcgcgcgcc tgccgaagtc gccgatcttt ttctcgatgt cggcgatcga
     7321 catctggtcg gcgtcgcgca ggatcggcac gacgagaccg cgcgggccgc cgaccgcgat
     7381 gccgatgtcg tagtagccgt ggtagacgat gtccgcgccg tcgatcgacg cgttgacgag
     7441 cgggaacttc ttcagcgcgt ggacggcggc cttcacgaag aaccccatga agccgagctt
     7501 gacgccgtgc tccttctcga agcgctcctt gtagcggttg cgcagctcga tcaccgcctg
     7561 catgttcact tcgttgaacg tcgtcaggat cgccgccgtc gattgcgact cgaccagacg
     7621 ctcggcaatg cgcgcgcgaa gccgcgacat cggcacgcgc tgctcgggac gcgcgccctg
     7681 cgtcacggct ggcggcgctg acggcggcgc ctttgcggcg ggtgctgcgg gagccgccgg
     7741 tggcgccttg gccgtcggcg ttggtggcgt tgcgggtgcg ctggcgcgcg tgccgagcgc
     7801 atcggcgacg tcgcccttcg tgatccggcc accgcgtccg gtgccggcaa cgctcgacgg
     7861 atccacgccc tgctcggcca tcatcttgcg ggccgacggg agcgcggtga cggtggcggc
     7921 cgtctccggc gccgtcgatg cagcggacgg cggctttgcc gatgccgctg gctgcgtggc
     7981 cgccggcgtg gatgcggagg cggcgtgctg cgcggccccc gtgtccgctt tcgtgtccgt
     8041 gtcgatcacg gcgatcacgt cgttcgacgt gacggtcgcg ccgtcgggct gcctgatttc
     8101 ggcgagcacc ccgtcgttcg gcgccggcag ttcgagcacg accttgtcgg tctcgatgtc
     8161 gatcaggttt tcgtcgcgcc tgacggcttc gccgggtttc ttgtgccagt tgacgagcgt
     8221 cgcctcggcg accgactcgg gcaactgcgg gaccttcact tcgattctca tcggccagga
     8281 ctcgtcgttg ggtcagcggg caaggagcat tccaccggaa cgcaacgtcg cggcgaatgc
     8341 atcctcgata agctgcttct gctcggcgtt gtgcttcgcc gcatagccca ccgccggcga
     8401 cgcggacacc ggccgcccgg cataggcgag gaccttgttc gcgtcgatgt cggcacgaag
     8461 atacgaattc agccggtacc acgcgccctg attctgcggc tcctcctggc accacacgac
     8521 ttccttcgcc gccttgtacc ggccgacctc gcgcttgtat tcgtcgtgcg gaaacgggta
     8581 ctgctgctcg agccgcagga tcgcgacgtc gccgatcttc tgctcgcggc gatacgcgcg
     8641 aagctcgaag tagaccttgc cggagcacac gatcacccgc cgcaccttcg caggatcgat
     8701 cgcgtcggat tcaccgatca cgttgcggaa ggcgccgctg gccagctcgt cgaacgacga
     8761 cacggcctcc ttgtgccgca gcaggctttt cggcgtgatg acgatcagcg gcttcctgaa
     8821 ctggcggatc atctgccggc gcaacagatg gaagatctgg gccggcgtcg tcggcacgac
     8881 gacctgcatg ttgtgctgcg cgcaaagctg caggtaacgc tcgggacgcg ccgacgaatg
     8941 ctcgggcccc tggccctcgt agccatgcgg caggaacagc acgaggtcgc agatgcgccc
     9001 ccacttgact tcgcccgcgc tgatgaactg gtcgatgacc acctgcgcgc cgttcgcgaa
     9061 gtcgccgaac tgcgcctccc agatcacgag ctgcgtcggc tcggacgtcg tgtagccgta
     9121 ctcgaatgcg agcaccgcgt tctcggtcag caccgagtcg atgatctcga aatcgggctg
     9181 gccctccttc acgtgctgca gcgggatcca ggtgccgagg tcccacttct cgcggttctg
     9241 gtcgtgcagc acggcgtggc gatgcgagaa cgtcccgcgg ccgatgtcct cgcccgacat
     9301 gcgcacgccg aaattctggt cgaggagcgt tgcgtacgac aggatttcgc ccatgcccca
     9361 gtcgaccggc agcttgcctt ccaccatcgc gcgccggtcg accatgattt tctcgacgcg
     9421 cggatgcagc ttgaaattcg acggaacggt ggtcgccttg atgccgagct cggtcaggcg
     9481 ggcgagcggc acgctggtgt cggcgggatc cgtccagcgc cggttcttgt aaggcgacca
     9541 gtcgacgatg aacggcggct tgtagttcga cagcaccgtg gtgttcgtgt ggtagccctt
     9601 gtccatcgcc gcgcggtagg tggcgatcat cgtgtcggcg tcgtcggcgc cgatcacgcc
     9661 ttcggcgatc agccgctcgg cgtagagctt gcgcgtgccc ggatgctggt tgatcttctt
     9721 gtacatcaag ggctgcgtga tcatcggctc gtcggcctcg ttgtggccga gccggcggaa
     9781 gcagacgaga tcgacgaaca cgtccttgtg aaacttctgc cggtagtcga gcgccagctc
     9841 ggttgcgagc aggcacgctt ccgggtcgtc gccattgacg tggaagatcg gcgcctcgac
     9901 catcttcgcg acgtcggtgc agtacagcgt gccgcgcacg tcgcgcgggt cggacgtggt
     9961 gaagccgatc tggttgttga tgacgatgtg gatcgtgccg cccgtgtagt agccgcgggt
    10021 gtgcgccatg ttgaggcact cctgcaccac gccctgcccg gcgatggcgg cgtcgccgtg
    10081 cagcagcacc ggcagcacct ggtcgccgcg cgcgtccttg cgccggtgct ggcgcgcacg
    10141 cacgctgccc tcgacgaccg gattgacgat ctcgagatgc gacgggttga acgcgagcgt
    10201 cacgtgcacc gggccgcccg gcgtcgagac gtcggacgag aagccctggt gatacttgac
    10261 gtcgccggcg gggaggtcct gcgccacctt gccctcgaat tcggagaaga ggtcgctcgg
    10321 catcttgccg agcgtattga cgagcacgtt gagccgcccg cgatgcgcca tgccgagcac
    10381 gatctcgccc accccggcag cccccgcgcg ctggatcaaa aggtcgagca tcgggatcat
    10441 cgaatcgccg ccctcgccgg agaagcgctt ctgcccgacg tacttcgtat gcaggtaccg
    10501 ctcgagcgtt tcggcggcgg tgagccgctc gaggatgtgg cgccgctcct cggggctgaa
    10561 cttcggcttc gagcggatcg gctcgaggcg ttcctgcacg aagcgcttcg tcggcgtgtc
    10621 ggagatgtac atgtattcgg cgccgaacgt gcgcgtgtac gtctcctcga gtgcggcgat
    10681 gatgtcgcga agacgcatcc gcgccgggcc cgccttgaac gagccgacgt cgaacggcgt
    10741 atcgagatcc gcgtcggtga agccgtaggt gcgaaggtcg aggtcgggaa tgtacgcagg
    10801 ctcctggcgc gacagcgggt cgaggtcggc gtggaacatg ccgagcgtgc ggtacttgct
    10861 gatgagccgc agcacgagca cctgcttgtg catcgtcgtc tcgtcgacca tcgcgcccgc
    10921 gacgcggcgg ctccgcgcga gctcgcggaa cgactcgact accgacgcgt gcggcacgtc
    10981 ggccgcatcg cccttgatcg cgtcgaagta gctgcgccac tcgggcgaga cgttcgcggg
    11041 atcggcgaga tacgactcgt acagctcctc gatgaacggc gcgttgctgc cgaagagcgt
    11101 gctgctcgct tcgagctctt gcatgcttcc catgattcac ctagcccaga ttcggggcgg
    11161 cggcgccgcc ccgtgtccat ccgtactcgc ggcctcgccg ccccgtgtcc actctatccg
    11221 cgttccgaga tgggcttcac cgtgcgccgt gcggccccgg tgaagagctg ccgcggccgt
    11281 ccgatcttgt actcgggatc ggacaccatc tcgttcaact gcgcgatcca gcccaccgtg
    11341 cgcgcgagcg cgaagatcgc ggtgaagagc tgcaccggaa tgccgatcgc cttctgcacg
    11401 atgcccgagt agaaatcgac gttcgggtac agcttgcgct ggacgaagta gtcgtcctcg
    11461 agcgcaatct tctcgagcgc catcgcgagc ttgaacagcg ggtcgtcgcc gagcccgagg
    11521 tcgcccagca cctcgtagca tgtctcgcgc atcagcttcg cgcgcggatc gtagtgcttg
    11581 tagacgcggt gtccgaagcc catcaggcgc acgttcgagt gcttgtcctt gacctgcgcg
    11641 atgaactcgc cgatcttcgc gacgccgccc tgcgcctgga tgtcgtagag catgttgagc
    11701 gccgcctcgt tggcaccgcc atgcgccggg ccccacaggc aggcgacgcc ggcggcgatc
    11761 gccgcgaacg ggttcgtgcc cgacgatgcg cacaggcgca ccgtcgacgt cgacgcgttc
    11821 tgctcgtggt cggcgtgcag gatgaagatg cggtcgagcg cacgcaccag cacgtcgttc
    11881 accacgtagt cctcgcacgg cgtcgcgaac atcatgcgca tgaagttccc ggcgtacggc
    11941 aggtcgttgc gcggatacat gtacggctgg ccgcacgagt acttgtaggc catggcgacg
    12001 agcgtcggca tcttcgcgat gagccggatc gcggagatgt cgcgctggtg cgcgtcgttg
    12061 aggttgatcg aatcggggta gaacgcgctc attgcgccca ccagtcccgt gagcacggcc
    12121 atcgggtgcg cgtcgcgccg gaagccgcgc atgaagaact gcatctgctc gttgaccatc
    12181 gtgtgattcg tgacgcggct cacgaagtcg tccttctgcg cccggttcgg cagctcgccg
    12241 tacaaaagca ggtagcaggt gccgaggtag tcggtgcgca ccgcgagctc ctcgatcggg
    12301 tagccgcgat agaggagctc gccgttgtcg ccgtcgatgt acgtgatcgc cgagctgcac
    12361 gcggccgtgg acatgaagcc cgggtcgtag gtgaatttgc ccgtcttggc gtacagcgtg
    12421 cggatgtcga tcacctgcgg gccgatcgtc ccggtgagga tcggaaacgt gaccgacggc
    12481 gagccgtcag aaaactggag ggtggcagtg gagtcggtca tcggacgtcc ttcggttcct
    12541 ggttcgggtt catccctgtg caaggcgcgc ggcgaacgac gcgaggcgcg gatcgtcggc
    12601 ttcggcccgg ccgctcagca aatcccacag tgcgttgtca gacatatgga gaagcgcgtc
    12661 cagatttgca atgtcgtctt cggtcagcgt atcggcgcag cggtcgagga agcgcgacag
    12721 gatgagatcg ttctccagca tgccgcgccg gcagcgccag cgaatgcgcc tcagtctcgc
    12781 attgtcgatc accattgcgc gcggaagacc ataatggggt cagagacgac attcggcgac
    12841 aaagccccgc gcattgcctc accgtgcatc gaatgtcgtc tctgacccct acacggtccg
    12901 cttcaccatg agatccttga tcttctcgat cgcaagcgtc ggattcaggt tcttgggaca
    12961 gacgtcgacg caattcatga tcgtgtggca gcggaacagc cggtagggat cctcgaggtt
    13021 gtcgagccgc tggttgacgt cgtggtcgcg gctgtcggcg atgaagcggt aagcctgcaa
    13081 caggccggcg ggaccgacga acttgtccgg gttccaccag aacgacgggc acgccgtcga
    13141 gcagcacgca cacaggatgc actcgtagag accatcgagc tgctcgcgcg cagccggcga
    13201 ctgcagccgc tcgcgctcgg gcggcggcgt gtcgttgacg agatacgggc gcaccgagtg
    13261 gtactgcttg aagaactggc tcatgtcgac gatcaggtcg cggatcaccg gcaatccggg
    13321 gagcggccgc agctcgacgg gctctttcag attcttgagg ttggtgacgc atgcgaggcc
    13381 gttcttgccg ttgatgttca tcgcatcgga gccgcatacc ccctcgcggc aactgcgccg
    13441 gaacgcgagc gaatcgtcgg ttgccttcag ccggatcagc gcgtcgagga gcatccggtc
    13501 gtgcggatcg agcgcgatgt cgtactcctg gagccgcggc ttcgcgtcct tctccggatc
    13561 gtagcggtag atgcggaatt tcatgcggac ctttggacgg tcagtaggtg cgcgccttcg
    13621 gcgggaacga ttcgacggac aacggggtga ggtgcaccgg cttgtaatcg agccggttgc
    13681 cctcgcgata ccacagcgtg tgcttcagcc agtgcacgtc gtcgcggttg ggataatcgc
    13741 ttcgcgcctg ggcgccgcgc gattcgtggc gcgcgagcgc ggacacgatc gtcgccttgg
    13801 ccacctcgat caggttgtcg agctcgagcg cttcgacccg cgccgtgttg aacgtgcggc
    13861 tcttgtcgtc gatcgcgctc cgcgcgacgc ggttctcgag ctccatcacc ttcgcgacac
    13921 cttcggccag cagtgcttcg ttgcgaaaca cgccgcaatg cgactgcatc gtcttgcgca
    13981 gctcgttgcc gacatccgca acccgctcgc ccgacgttgc cgcgtcgagc cgcgcaagcc
    14041 gcgcgagcga cgcatcgccg gcgtccggcg gcagcgcctt gtgcgcaagc gccgccaggt
    14101 tgcggctcac gatgaaatcg ccggcggcgc ggccgaacac gagaaggtcg agcagcgaat
    14161 tcgtgccgag ccggtttgcg ccgtgcacgg aaacgcacgc gcattcgccg accgcgtaca
    14221 ggccctcgac gacgacgccg ggatcaccgt gcctgggtgc gacgacctgc ccgtggatgt
    14281 tcgtcgggat gccgcccatc atgtagtgga tcgtcggcac gaccgggatc ggctccttga
    14341 tcggatcggc gttcgcgaac ttgatcgcga tctcgcggat cgacggcagc cgcttcatga
    14401 tgacgtcggg gccgagatgg tcgagcttca ggagcacgta gtccttgtgc ggaccgcagc
    14461 cgcgcccctc cttgatctcc tggtccatcg agcggctgac gatgtcgcgc gacgcgaggt
    14521 ccttcaggtt cggcgcgtag cgctccatga agcgctcgcc attgcagttg agcaggatgc
    14581 cgccctcgcc gcgcaccccc tcggtgatca gcacgccggc gccggcgacg ccggtcgggt
    14641 ggaactgcca gaactccatg tcctcgagcg gcacgcccgc gcgcgccgcc atgccgaggc
    14701 cgtcgccggt gttgatgaac gcgttggtcg acgcggcgaa gatgcgtccg gcgccgccgg
    14761 tggcgagaac cgtcaccttc gcctcgagcg caaccacgtc gccggtctcc atgtcgagcg
    14821 ccgtcacgcc gacgacgtcg ccgtcggaat cgcggatcag gtcgagcgcc atccactcga
    14881 cgaagaattg cgtatgcgcg cgcacgttct gctggtagag cgtgtgcagc atcgagtggc
    14941 cggtgcggtc ggccgacgcg cacgcgcggc gcaccgactt ctcgccgaag ttcatcgagt
    15001 gcccgccgaa cgggcgctgg tagatcgtgc cgtcggggtt gcggtcgaac ggcatgccga
    15061 agtgctcgag ctcgtagacc acctgcggcg ccatccggca catgtattcg attgcgtcct
    15121 ggtcgccgag ccagtcggag cccttgaccg tgtcgtacat gtgccacagc cagctgtcct
    15181 cggacatgtt gccgagcgat gcgccgatgc caccctgcgc cgcgaccgta tgcgagcgcg
    15241 tcgggaacac cttcgacagc accgcgacgg tgagcccggc agcggccagc tgcagcgacg
    15301 cgcgcatgcc tgcgccgccg gcaccgacga tcagcgcgtc gaagcgtcgc gtgggcagct
    15361 tcatcgaagg ggcggcaccg cggcgcgcat cacggattgc cccacaggac gccgatcgtc
    15421 cacccgccat acgcgacgag caggcagacg acgacgatct cgagcatcag gcgcacgccg
    15481 tacggcttcg cgtaatccat caggatgttg cgcacgccca cccacgcatg ccagaagagc
    15541 gacgccatga acaggaacgt cgcgacgcga aatgcgttgc cggcgaaaag cgcccgcgcc
    15601 gtcgggtaat cgagaccgcc gtgccacagc acgatgccga ggacgagcgc ggtgtacagc
    15661 gtgatgacga tcgccgtcac gcgctgcgac agccagtcgc gcaggccgta gtgggcgccg
    15721 accggcagct tgtcggtgcc ggctaccaca gccgcaccgc gacgatcagc gcaagcagga
    15781 tcgaaagcac cagcacgagc gccgccgatc cccgtgcggg cgcgaggccg atgccgatgt
    15841 ggaggtcgag ggcgagatgg cgcagcccgg cgagcaggtg aaacaggaag caccacgcaa
    15901 gcaccaggaa cacgagcttg ccgaacgggg tgccgagcgg cgcggtggcg gcggcgaaac
    15961 cttcgggtga tgcgagcgat cgctgcacgt cccacagcag cagcggaatg ccggcgatga
    16021 acagcagcgc cccgcttatt cgatgcagga tcgacgcgat cgccggcaac ggctggcgga
    16081 tcgcgaacag gttgaggtag accgggcgcg gcttgtcgac agggcgatcg gcggcggcca
    16141 tgcctcagcc tcggtgagga acgcgtcgca aaagcacggg aaatccgctg caccgtgccg
    16201 cgacgctgca ttcgaatggt ttttgcacag catcaaatta tagccctgcc aacgggcagg
    16261 ccgccgcggc aaccgaacag ggctcgaaga cgacgttcgg gatgccggtc ccgtaggcgg
    16321 tcatgggaac cgcaaaaggc gtccggccct ccactttcac gtgagcgcgt tccagtagtg
    16381 ctcgtggcgc gtcgtgcaga ggccacggcg cacttcgacg gggcgatcgc cgtaggtgta
    16441 ggcgacacgc tcgaccgcga gcagcggcgc acccgctgca acgcgcagca gcgcagcggt
    16501 tgcc
//
LOCUS       DASSVX010000201        36910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_391703, whole genome shotgun sequence.
ACCESSION   DASSVX010000201 DASSVX010000000
VERSION     DASSVX010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 36910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36910
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391703"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFO53_08475"
     CDS             complement(<1..194)
                     /locus_tag="VFO53_08475"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478497.1"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET8876349.1"
                     /translation="MHRTLALLGATGSIGDSTLDVVARHPERFSVAALAANRNHAKLL
                     GLCRRFRPRVAALADGDAAHA"
     gene            complement(194..1036)
                     /locus_tag="VFO53_08480"
     CDS             complement(194..1036)
                     /locus_tag="VFO53_08480"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET8876350.1"
                     /translation="MASALATRIATGIVLIAAILAALLLLPTLVFGGVVLLIVLVAAS
                     EWAKLIGLADGARTGFVIAVLAAGCALLRLGALRGFDPVVALACGAATLFWLVVGVPS
                     VLRNWQPASVLVRAVSAFVMLEGAFVAIVALQDRSPWLALAAMAIVWLADTAAYFAGR
                     RFGRRKLAPQISPGKTWEGVHGALLAVAVYALLLLPLARRAGYEGGFDARAIVAWVAF
                     AIALAALSVVGDLHESLLKRRAGVKDSGAILPGHGGVLDRTDALLAAMPPVALAATVF
                     LGAA"
     gene            complement(1089..1898)
                     /gene="uppS"
                     /locus_tag="VFO53_08485"
     CDS             complement(1089..1898)
                     /gene="uppS"
                     /locus_tag="VFO53_08485"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET8876351.1"
                     /translation="MFTSSTRIIPESRDVPRHVAIIMDGNGRWASRRLLPRVAGHRKG
                     VEAVRAAVRASIEHGVEYLTLFAFSSENWRRPAEEVSILMELFTRALEAEVAKLHKNG
                     IRFKVVGDTSRFDPRIRDLVAAGESLTANNTRLTLTVAANYGGRWDIAQAARRYFAAN
                     ADAPASARAFVPEVLEPYLAMAYAPEPDLFIRTGGEKRISNFLLWQLAYTELYFTDLL
                     WPDFDASALAAAIASYAGRERRFGRTSEQVQAQNDAQIEARLPQRVQAQDA"
     gene            complement(1933..2490)
                     /gene="frr"
                     /locus_tag="VFO53_08490"
     CDS             complement(1933..2490)
                     /gene="frr"
                     /locus_tag="VFO53_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET8876352.1"
                     /translation="MISDVRNSAEQKMARSIETLKADLAKVRTGRAHTGLLDHIHVDY
                     YGSSMPLSQVANVTLADPRTIGVTPWEKKMIPTVEKAIRDSDLGLNPATSGEMIRVPM
                     PALTEERRKELIKVVHKEAEAAKIAVRNVRRDANEQLKKLLKDKQCSEDDERRAHDEL
                     QKLTDRFVADVDRLLQAKEADLMAI"
     gene            complement(2590..3366)
                     /gene="pyrH"
                     /locus_tag="VFO53_08495"
     CDS             complement(2590..3366)
                     /gene="pyrH"
                     /locus_tag="VFO53_08495"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET8876353.1"
                     /translation="MSPPDSAVTAAIAPLPGIAAAPAPAYRRILLKLSGEALMGSVSY
                     GIERETIDRIVAEVAEVVRLGVQVAIVIGGGNIFRGVAPGAAGMDRATADYMGMLATL
                     MNALALQDALRRAGVESRVQSALRIDQVAEPYIRGRALRHLEEHKVVIFAAGTGNPFF
                     TTDTAAALRGREMGVDIVLKATKVDGVYTSDPLKDETARRYSTLTFDEAIVRNLKVMD
                     ATALALCRDQNMLLKVFSIFVPGALKRVVMGGDEGTLVHC"
     gene            complement(3399..4277)
                     /gene="tsf"
                     /locus_tag="VFO53_08500"
     CDS             complement(3399..4277)
                     /gene="tsf"
                     /locus_tag="VFO53_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET8876354.1"
                     /translation="MPEISASRVMELRQRTGLGMMECKKALTEAAGDLGKAEELLRIR
                     SGAKASKAASRVAAEGVVGIAVAADARTAAMVEVNCETDFVARNDEFRAFANEVARIV
                     VREQPADLDALAGRTLASGDTVEARRIAMVQKIGENIALRRFVRLAAEGRVASYVHGT
                     RIGVLVDYTGGNEALGKDLAMHIAATKPIAVSREQVPGAVVEKERSIAAARAAESGKP
                     ANIVEKMVEGAVAKFLAEVTLLPQPFVKNDKETVGRLVQAQGARVNGFILYVVGEGIE
                     KRNDDFAAEVAAMTRS"
     gene            complement(4398..5132)
                     /gene="rpsB"
                     /locus_tag="VFO53_08505"
     CDS             complement(4398..5132)
                     /gene="rpsB"
                     /locus_tag="VFO53_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET8876355.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRYWNPKMADYIFGQRNKIHIVNLEK
                     TLAMYNDAMKYVRQLAQNRGTILFVGTKRQAREIIAEEAQRCAMPYVDHRWLGGMLSN
                     FKTVKQSIKRLKDLETMKQDGTFDRMSKREALSHQRELDKLEKSLGGIKDMNALPDAV
                     FVIDIGYHKIAVTEAKKLGIPLVAVVDTNHNPDPIDYVIPGNDDSSRAIRLYARGVAD
                     SVLEGKTSTIQEIVATGDEFVDEATA"
     gene            5478..6467
                     /locus_tag="VFO53_08510"
     CDS             5478..6467
                     /locus_tag="VFO53_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HET8876356.1"
                     /translation="MTPPAPAACNHTGWSVLRVSGDDALAFLHAQLSSDVQALAPGEG
                     QYWCYNSPKGRMLANGVLWRAPADVPDAAITLLLAGDVAGAIRRRLAMFVLRAKVRLD
                     DASATSMLIGIAGDGSADASRQAFGVSPPPGRAVAFAGGASAFTLPDRRIAIVAPQAE
                     RPVLHAALARHASVVTNDVWRWFGVAAGVPWITAATSDLFVPQMANWDLLGGVSFRKG
                     CYPGQEIVARMQYLGRLKERLFAFHADALDAVPGTRLYSAAFGAEQACGTVVNAAPDL
                     VAGGVALLAVVQLAAVDANDVALGEPGGARLVRRGLPYDVPAGAPGAAPRPSG"
     gene            6471..7313
                     /locus_tag="VFO53_08515"
     CDS             6471..7313
                     /locus_tag="VFO53_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HET8876357.1"
                     /translation="MCLAVIALDAHPRYAVVIAANRDEFHARPSAPAHWWTGAHGNAL
                     LAGRDLEHGGTWLGVARNGRFAFVTNVREPGRHDPGAPSRGVLVPALLADPRPIGVAL
                     QELIADARGYNGFNLVAADTAGAAFGSNRGAHTVPLGRGLHGVSNAGLDTPWPKLVRA
                     KAGLAAWIAADDDTPDPLWQVLADPTPAADDELPDTGLPRERERLLSSPFIMSETYGT
                     RCSTLVTIARDGQVEFSERSFDAAGTTRGEARFHFRLDQSARSITQCAMPASSNTSPR
                     ATNP"
     gene            complement(7238..7663)
                     /locus_tag="VFO53_08520"
     CDS             complement(7238..7663)
                     /locus_tag="VFO53_08520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8876358.1"
                     /translation="MKRFTVGICGWTDAADALRKVRHDVFVVEQRVPESLEWDAADAT
                     SLHALATDTSGEPIGCARLLPDGHIGRVAVLAPWRGCGVGSALLQRLVDAARARGAPR
                     VMLNAQVAAMPFYARYGFVARGDVFDEAGIAHCVMERAL"
     gene            complement(7660..8556)
                     /locus_tag="VFO53_08525"
     CDS             complement(7660..8556)
                     /locus_tag="VFO53_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET8876359.1"
                     /translation="MSTSLDITIVAPAGFATDPAAVDRAEALLARLGHRVQSDATARG
                     RWQRFSAPDDERLAAVERVAADPRTDVAVMLRGGYGFSRLIDRIDFAALARAGKRWMG
                     HSDFTAFQLAALARSGIVTYAGPMAASDFGAVTPSAFTLRNCFGVLGNDEWSIDCALD
                     GPSHSCAGTLWGGNLAMLAHLVGTPYFPRIDGGILFLEDVGEHPYRIERMFHQLLHAG
                     VLARQRAVLLGAFTEYQLHANDAGYDLDAAIAQLRSRVSVPVHAGLPFGHVPDKLTLP
                     VGGHCALDVDAGRARLVLSRYR"
     gene            complement(8553..9020)
                     /gene="tadA"
                     /locus_tag="VFO53_08530"
     CDS             complement(8553..9020)
                     /gene="tadA"
                     /locus_tag="VFO53_08530"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HET8876360.1"
                     /translation="MPATDDDWMAHALTLAAEAEARGEVPVGAVVVCDGAIVGRGGNA
                     PIAGNDPTAHAEIAALREAAAALGNYRLPGCTLYATLEPCAMCAGAIMHARVARLVFG
                     ARDPKTGACGSVVDLFAEPRLNHHTRVTDGVAADECARLLTRFFTARRQRATG"
     gene            complement(9007..9408)
                     /locus_tag="VFO53_08535"
     CDS             complement(9007..9408)
                     /locus_tag="VFO53_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876361.1"
                     /translation="MTARGRTARNAQGQVAPPPARPLAGALASRTTFLSRIRRAATYA
                     LGLVVITLAVGMIGYRALEHLSWLDAFHQSAMLLSGMGPVVDMKSDAGKIFDGVYALF
                     CGVILLASTGLMFAPVIHRLLHRFHIEDARD"
     gene            complement(9461..11083)
                     /gene="guaA"
                     /locus_tag="VFO53_08540"
     CDS             complement(9461..11083)
                     /gene="guaA"
                     /locus_tag="VFO53_08540"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET8876362.1"
                     /translation="MHRQGSKVVSGPTFPHCRILILDFGAQYTQLIARRLRETDVYCE
                     IHPWTVGDDFVRAFDARGIVLSGGPASVTTGETPRAPAAVWTLGVPVLGICYGMQTMA
                     EQLGGKVEAGRVREFGRAEVRARGHTTLLRDIEDRRNADGHGLLDVWMSHGDKVVGLP
                     PGFTLAGSSDACTIAAMADESRRFYGVQFHPEVTHTPQGASLLARFAHDICGCGHDWT
                     MHGYAAEAVDAIRAKVGGDEVLLGLSGGVDSSVAAALLHRAIGDRLVCVFVDHGLLRQ
                     NEAEQVLDTFAMHLGVRVVHVDASNQFLAALAGVDEPEAKRKIIGRLFVDVFQREAAK
                     LTDAKWLAQGTIYPDVIESASAKGSSAHTIKSHHNVGGLPETLHLKLLEPLRELFKDE
                     VRKLGLELGLPRDMVFRHPFPGPGLGVRIPGEVTRERADILRAADAIFIDELRAARDD
                     AGQSWYDAVSQAFAVFLPVRSVGVMGDGRTYEHAIALRAVQTTDFMTADWAPLPHALL
                     ARISNRITNEVRGINRVVYDVSSKPPATIEWE"
     gene            complement(11127..12587)
                     /gene="guaB"
                     /locus_tag="VFO53_08545"
     CDS             complement(11127..12587)
                     /gene="guaB"
                     /locus_tag="VFO53_08545"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET8876363.1"
                     /translation="MRVVRKALTFDDVLLVPAHSSVVPRDVSLVTRLTRTISLNIPLL
                     SAAMDTVTDARLAIAIAQEGGLGIVHKNMAPARQAAEVAKVKRFESGVVKDPITIPPS
                     MSVRQVLALTHQYRISGLPVVDGRKVVGIVTNRDLRFETNLDQAVSQIMTRAEKLVTV
                     PEGTDLETAKALMHRHRLERVLVVNREHELRGLITVKDILKSTEHPIACKDDLGRLRV
                     GAAVGTGEDTDARVEALVAAGVDVIVVDTSHGHSQGVLDRVQSVKKRYPQVQVIGGNI
                     ATASGAKALVEHGADGVKVGIGPGSICTTRIVAGVGVPQVSAIQDALSGAGDVPVIAD
                     GGIRYSGDIAKAIAAGASVVMLGSLFAGTEESPGDIELYQGRSYKSYRGMGSIGAMQL
                     GSSDRYFQDAEGEGAAEKLVPEGIEGRVPYKGSLANVILQLMGGLRAAMGYCGCPTIR
                     DLSTRAEFVEITSAGIRESHVHDVQIVKEAPNYRVE"
     gene            12759..13217
                     /locus_tag="VFO53_08550"
     CDS             12759..13217
                     /locus_tag="VFO53_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET8876364.1"
                     /translation="MSRASLYAFAVLLLAAGHSASGAAALYKWTDAQGRIVYSDQPPQ
                     ANVKTEQLRGAPPPANPNAAKELAQREADFRKRQADAEKAAAKAGKESASSADHAESC
                     AQAKGQLKQLAESQLAIYRYNEKGEREVMDEDARGRERAKLNAWIRDNKC"
     gene            complement(13222..15126)
                     /locus_tag="VFO53_08555"
     CDS             complement(13222..15126)
                     /locus_tag="VFO53_08555"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET8876365.1"
                     /translation="MFDLLHKHPRVTQAVLALITLPFAFFGVDYYFRGGDSGQTVASV
                     GKNKITQAEFDDALRDQQQRMRQALGSNFDPAMLDSPEMRYALLDQLVNQRLLENEAR
                     AGRFRVTDAQLAQFIAALPPFQEDGKFSADRYREVLASQNMSPLMFEQRVRGDLVLSP
                     LQDPIIGGSIAAKSSVQRYLTLLDQKRDVAIAQVAAEPFEKSVKVSDDDVKAFYDKNP
                     KAFATPEVAKVDYILLTQDAMAAQVKVDPAEVKQAYEANAKQYTTSEERQASHILIAV
                     KKDASDADKAKAKQKAAELAEKARAKPDSFAELAKANSQDPGSAAQGGDLGSFARGAM
                     VKPFEDAVFAAKVGDIVGPVQTDFGYHVIKVTGITPPHVQSFDEVKGRIEAELKRQKA
                     AQKFASAADQFQNLVYEQADSLAGAAKTLDLKVETTPLVTREKMQSIAMGSAKFVQAL
                     FSPESVQSKRNTEAIEVGPNALMAGRIVEYKPAAPKPFAEVRDEIRQQLIHQRASELA
                     VKTGREKLALLEAGKDAGVAFDKPVTLTRNQAQPGVTADGLKKIFDAGERKLPEYAGA
                     SNERGGFSIYRIDKVIDPPAPDAAKVDAASARVGSELGRELMNAYLASLKADTDVKIN
                     QAALEKKQNQ"
     gene            complement(15201..15277)
                     /locus_tag="VFO53_08560"
     tRNA            complement(15201..15277)
                     /locus_tag="VFO53_08560"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15241..15243),aa:Asp,seq:gtc)
     gene            complement(15428..15503)
                     /locus_tag="VFO53_08565"
     tRNA            complement(15428..15503)
                     /locus_tag="VFO53_08565"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15468..15470),aa:Val,seq:tac)
     gene            complement(15527..15799)
                     /locus_tag="VFO53_08570"
     CDS             complement(15527..15799)
                     /locus_tag="VFO53_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET8876366.1"
                     /translation="MNKSEMIDAIARHADISKAAAGRALDATVGAIKTSLRKGDIVTL
                     VGFGSFYVGKRSPRAGRNPRTGDAISIKGGKVPKFRAGKALKDALN"
     gene            complement(16002..18446)
                     /gene="lon"
                     /locus_tag="VFO53_08575"
     CDS             complement(16002..18446)
                     /gene="lon"
                     /locus_tag="VFO53_08575"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET8876367.1"
                     /translation="MPNDTSLPTGVTTLPLLPLRDVVVFPHMVIPLFVGRPKSIKALD
                     VAMDAGKHILLVAQKQAAKDDPAADDLYGIGSVATVLQMLKLPDGTVKVLVEGTQRAH
                     IRSIEEHGEYLAAHTQPIPGDPGTAHEVEAMRRALLTQFDSYVKLNKKIPPEILTSMS
                     GIDDGGRLADAIAAHLPLKLEQKQQILEMSDVGKRLEHLLGVLEGEVDILQVEKRIRG
                     RVKRQMEKSQREYYLNEQVKAIQKELGEGDENADLEEMEKKIRAAKMSREAENKAMTE
                     LKKLRMMSPMSAEATVVRNFIDTLLGLPWRKKSKINKDLAHAVKVLDADHYGLEKVKE
                     RIVEYLAVQQRVDRLKAPILCLVGPPGVGKTSLGQSVAKATGRKFVRMSLGGVRDEAE
                     IRGHRRTYIGSMPGKILQNMTKVAVRNPLFLLDEVDKMGQDFRGDPSSALLEVLDPEQ
                     NNTFVDHYVEVEYDLSDVMFVATANTMNIPAPLLDRMEVIRLSGYTEDEKIAIAKQYL
                     LPKQIKHNGLKPEELHVAESALRDIVRYYTRESGVRNLERDISKICRKVVKALLLKQS
                     EGKARKARGPMEVSSKNLDKYLGVRRYTYGIAERRNQVGQVTGLAWTEVGGDLLTIES
                     VALPGKGKTTSTGKLGDVMNESIAAALSVVRHRSKELGIAPEFYTKTDLHIHLPEGAT
                     PKDGPSAGTAIATSMVSTLTGIPVRCDVAMTGEITLRGEVLPIGGLKEKLLAAGRGGI
                     KTVLIPEENVKDLVEIPDEIKNRLDIHPVRWIEQVLERALERQPTPITDDADKAQAAV
                     PPATPAADDKSAVIKH"
     gene            complement(18810..20081)
                     /gene="clpX"
                     /locus_tag="VFO53_08580"
     CDS             complement(18810..20081)
                     /gene="clpX"
                     /locus_tag="VFO53_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET8876368.1"
                     /translation="MAEKSSSGDKLLYCSFCGKSQHEVRKLIAGPSVFICDECIELCN
                     DIIREEGTGSETARADRSKLPTPHEIRQILDQYVIGQEPAKKILSVAVYNHYKRLESG
                     AKDDEVELAKSNILLIGPTGSGKTLLAQTLARLLDVPFVIADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDIEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     IASVPPQGGRKHPNQEFVQVDTTNILFVCGGAFDGLEKIIRQRTAKTGIGFGAEVASP
                     DDRRSMTILLRGTEPEDLIKFGLIPEFVGRLPVVATLDELDEAALIQILVEPKNALIK
                     QYHKMFRMEGVELEIRELALRAIARKALARKTGARGLRSILEQVLLDVMYDLPSMSNL
                     ARVVVDESTIANDGKPLLMFAETAKVAASAK"
     gene            complement(20112..20723)
                     /gene="clpP"
                     /locus_tag="VFO53_08585"
     CDS             complement(20112..20723)
                     /gene="clpP"
                     /locus_tag="VFO53_08585"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HET8876369.1"
                     /translation="MEEPRGLGLIPMVIEQSGRGERAYDIYSRLLKERVVFLVGPVNE
                     STANLVVAQMLFLESENPDKDIHFYINSPGGSIAAGFAVYDTMQFIKPDVSTLCVGMA
                     ASMGAFLLAAGAKGKRFALPNSDVMIHQPLGGFQGQASDVEIHAKYILSLRQRLNVLM
                     AMHTGKPVEQIARDTERDNFLTAEQARDYGIVDKVLQKRLLPE"
     gene            complement(20856..22175)
                     /gene="tig"
                     /locus_tag="VFO53_08590"
     CDS             complement(20856..22175)
                     /gene="tig"
                     /locus_tag="VFO53_08590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET8876370.1"
                     /translation="MQTTLETLGALERRLNVAVPLADIEGEVKKRLSRLARTAKVAGF
                     RPGKVPLRMLDQQYGPQVRSDVITERVQSTFNDAVRAQNLRVAGVPRIEPRSGDAPAP
                     DALQFSAVFEVYPEVKVADVATIEVSRPRAEVTPADVERTLGVLRKQRTTYTPVERAA
                     QGGDRVQVDFTGTIDGVEFPGGQAKNFPIVLGEARMLPEFEAAVTGMSAGETVEFDLT
                     FPADYHGREVAGKTAHFSLRVAGVEEPHLPPLDADFAGAFGIASGETDDLRREVEANL
                     KLELKRKIESTIKAQALQGLRKQASFAIPKALVESEAQLLRERALANLREQQNVKAEG
                     LDVSVDLFRPQAEERVALGLIIGELVRSENLHAKPEQVRALVTETAQTYEQPDAVVRW
                     HYEKPERLRDFEAMVLEQNVVDWVLARAKVTDAPATFEQVMAPQTGA"
     gene            complement(22194..22278)
                     /locus_tag="VFO53_08595"
     tRNA            complement(22194..22278)
                     /locus_tag="VFO53_08595"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22242..22244),aa:Leu,seq:tag)
     gene            22392..23225
                     /gene="hpnC"
                     /locus_tag="VFO53_08600"
     CDS             22392..23225
                     /gene="hpnC"
                     /locus_tag="VFO53_08600"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HET8876371.1"
                     /translation="MSVGHYENFPVASVLVPARYRDAVIAIYRFARTADDIADEGGAA
                     PADRHRALDAFSRRLDAIERGETPAEPPFADLALAVQRHQLPIARLRDLLSAFAQDVD
                     VTRYPTYDDVLDYCRRSANPVGRLLLALYGIDDAGALAESDAICTALQLANFWQDVAI
                     DWRKDTRLYLPLEDLACFGVPLSHVANGVCDARFSRLMAFETARTRALFLSGRPLIRR
                     LPWRLGLELTAVIAGGMRVLERIDAVRGDVFTQRPVLGKRDWALLALRMLAPSAAVRF
                     A"
     gene            23222..24055
                     /gene="hpnD"
                     /locus_tag="VFO53_08605"
     CDS             23222..24055
                     /gene="hpnD"
                     /locus_tag="VFO53_08605"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HET8876372.1"
                     /translation="MTPNEYCAQKVAQSGSSFYYSFRLLPPPRRAAISALYAYCREVD
                     DVVDEVSDPSVARVKLAWWRTEVDAIFTGTPQHPVALALADAAERYGLAQSNLQTVID
                     GMQMDLERARYVDFAELETYCDRVAGVVGVMSAAIFGYEDPSTLVYARRLGVAFQLTN
                     IIRDVGEDARRGRIYLPQDELARFGVAPSDLLRARSSPAFVGLMRFQTERASAWYDTA
                     LAALPERDRRAQRAGLAMAAIYRTLLGEIARDGYRVLDRRIALTPLRKLWIATRAAWR
                     A"
     gene            24075..25466
                     /gene="hpnE"
                     /locus_tag="VFO53_08610"
     CDS             24075..25466
                     /gene="hpnE"
                     /locus_tag="VFO53_08610"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HET8876373.1"
                     /translation="MSARDRRSVVVIGGGWAGCAAAVELARCGHSVELHEAAIALGGR
                     ARAVVRDGLPLDNGQHLLLGAYTDTLAFAAAIRDRTSSAWTIEPLALRPFARAQRNTL
                     SLSTRHAPAPLALLAGLVRARGLPIGARIATIRWFVRQRRNGWQCAPDLTVAGLLADA
                     PAVVRDDLWHPLCLAALNTPPARASAQVFLNILRETFGAGAHATSLVLPREGLAEAVP
                     EAAARWLAANGHPVRRSTRTRIRRIDADGVLLEASGEHARADAVIVAVGPHQLASAFA
                     PGIERDHPSIVQALASVARFDYEPITTVYLGYAAARALPRGLLRLDDAPGQWIFDRSD
                     ILERAAASATRAPMRSLVSVVISARGPHSALDHAALVGATDGQLRRLAPDLAPLVWSR
                     VIEEKRATYACVPGLARPACGRLVERVGLAGDYAYAAFPATLEAAVRSGRIAAHAIIG
                     APSARSLDPHACG"
     gene            complement(25526..25966)
                     /gene="smpB"
                     /locus_tag="VFO53_08615"
     CDS             complement(25526..25966)
                     /gene="smpB"
                     /locus_tag="VFO53_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET8876374.1"
                     /translation="MSIVENRKAFHDYFIEERFEAGIALEGWEVKAIRGGRANLKEAY
                     VVVKDGGLMLIGAHIAPLTTASTHVTPDPTRTRRLLMHREEINRLIGKVERAGYTLTP
                     LDLHYAKGRVKLAVGLAKGKKQHDKRAAIKEREWNREQQRLLRG"
     gene            26066..26494
                     /locus_tag="VFO53_08620"
     CDS             26066..26494
                     /locus_tag="VFO53_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HET8876375.1"
                     /translation="MRAVKKSVLVPYSAAQMFELVDRVELYPQFLPWCGGTQVLEQGE
                     NRKTARIDIDYHGVRAHFTTGNVNDPPRSIVVTLKDGPFRHLHGEWRFIALSEHGCKV
                     EFDLAYEFATSLLDRVIGPVFSHIANTFIDAFVKRAESVG"
     gene            26569..26838
                     /locus_tag="VFO53_08625"
     CDS             26569..26838
                     /locus_tag="VFO53_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HET8876376.1"
                     /translation="MRVQVAFVADAQQTLATLDLPQGSRVSDAVAQSGLGLVDDVNCG
                     RLVCAIFGRRADHDTPLREGDRVEITRPLVCDPKTARHRRAAQGR"
     gene            complement(26847..27497)
                     /locus_tag="VFO53_08630"
     CDS             complement(26847..27497)
                     /locus_tag="VFO53_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET8876377.1"
                     /translation="MTVDRTRRRVLATAVLVALPLAAAGEPLLDELALAVENDRVDEV
                     KKLLARGLDADSVAASGEPMLCIAARNGYAQTAQTLLAFKAHPNTPNAYGDTPLMLAA
                     LRGHLDTVRVLQAGGADLNRRGWTPLIYAATGGHDDIVTFLLLHGADIDAQSPNGTTA
                     LMMAIHEHHPDTARLLIAHGADVSLRNQDGATALAWAMRGNESDTVKELRRVGARE"
     gene            complement(27487..28263)
                     /locus_tag="VFO53_08635"
     CDS             complement(27487..28263)
                     /locus_tag="VFO53_08635"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET8876378.1"
                     /translation="MFVDSHCHLDFPELRDDLPQVLDAMRESRVTHALCIGVEMGAWP
                     AVHALALEHANLYASVGVHPDYEDSDEPGVDLLAARAHERKVVAIGETGLDYFRRSGD
                     LEWQRARFRTHIRAAREAGRPLVIHTRAAADDTIAIMREERAGDAGGVMHCFTETWDV
                     AQRAIELGFYISFSGIVTFRNATALKDVAKRVPLSRMLIETDSPYLAPVPHRGKRNQP
                     AWVRHVAEEIAALREVPVEAIAQATSDNFFRLFGIGRDDG"
     gene            complement(28368..28742)
                     /locus_tag="VFO53_08640"
     CDS             complement(28368..28742)
                     /locus_tag="VFO53_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET8876379.1"
                     /translation="MADKIPGPTPALARPGVLSLNIREKAALYAAYMPFLKGGGIFIP
                     TSRPYTLGEEVFMLLSLMDDPNRIAVQGKVVWITPDGGQGNRTQGIGVQFTLDDTGAA
                     AKATIERILGEHLASTRATHTM"
     gene            complement(28745..29782)
                     /gene="holB"
                     /locus_tag="VFO53_08645"
     CDS             complement(28745..29782)
                     /gene="holB"
                     /locus_tag="VFO53_08645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET8876380.1"
                     /translation="MTRDGEGRPAEPARAPLPWQRDVVASLLSRRAQWPHALLVTGPV
                     GIGKRMLAQALAGALLCESPLAGGAACAECAGCRYVAAGQHPDLRTVEPVELDDDGDA
                     KIVEWIPVDRIRALTRWAAITSHRGGAKVALIVPAERMNEAAANALLKTLEEPPDGTC
                     LLLVSHLPGRLPATIASRCQRIVAPRPTRAQARAWLVQQGTDDPDAALAQAHGAPLRA
                     LALADRAYQGERSAWIRAFAAPATLEVAGMGARIDAGPRDQRKDRLAGVIDWLLGWCA
                     DLACVHAGGQPLENVAFEAALRELACAVAPPALFRYHRQLLRQRALLAHPLSPRLVAE
                     ALMIDYRALFG"
     gene            complement(29779..30411)
                     /gene="tmk"
                     /locus_tag="VFO53_08650"
     CDS             complement(29779..30411)
                     /gene="tmk"
                     /locus_tag="VFO53_08650"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET8876381.1"
                     /translation="MPDQRPRGRFITLEGIDGAGKSTHARFLAETLVARGKRVVATRE
                     PGGTSAGEAIREWLLHRALSHDAEALLMFAARREHVEQVIEPALARGDIVLCDRFTDA
                     TYAYQGAGHGVSLAMIDALAAWVHGQCNPELTLLFDVPSEVSRERLARMHAKGKAADK
                     FERETASFFGRVRDGYLARAAAEPARFRIIDSTRPVAEVRAELCSIVDTL"
     gene            complement(30456..31460)
                     /gene="mltG"
                     /locus_tag="VFO53_08655"
     CDS             complement(30456..31460)
                     /gene="mltG"
                     /locus_tag="VFO53_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET8876382.1"
                     /translation="MRKSRVATVVVLLLVASLVVAAAIGVALYRKPLALPHTPFDFEV
                     RSGASLASVARSLHAAGVVPHAIALTALARVMHVDRTIKAGSYEIEQGITLPRLLAKL
                     TQGDVTQTAITIVEGTTFGQLKRALDDNPDVAHQTDGLSDAELLARLGATEPAPEGLF
                     FPDTYYFAAGTSDIAVLKRAYRALHARLDAAWSHRAPDLPLASPYDALILASLVEKET
                     GHAQDRPLIASVFVNRLARGMRLQTDPAVIYGIGAHFDGNLRKRDLEADSPYNTYVRT
                     GLPPTPIALPSQASLDVVVNPPATPYLYFVSRGDGTSQFSANLIEHNRAVSKYQRGGR
                     "
     gene            31522..32034
                     /gene="gspG"
                     /locus_tag="VFO53_08660"
     CDS             31522..32034
                     /gene="gspG"
                     /locus_tag="VFO53_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HET8876383.1"
                     /translation="MADYNGPMIALAPHRSLRDRVGLHRCRAPGRERARGFTLIEIMV
                     VIVILGVLAALVVPSVLSRTDDARIVAAKTDLGAIRQALKLYRLDNQRYPTTEQGLTA
                     LATKPTEPPVPPNWKPGGYLEKVPLDPWGRSYQYLNPGLKSDVDVFTYGADGAPGGTG
                     ADADIGSWDL"
     gene            32101..32607
                     /locus_tag="VFO53_08665"
     CDS             32101..32607
                     /locus_tag="VFO53_08665"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HET8876384.1"
                     /translation="MTPVSPTSRTGGFTLVEILVVIVILGIVAGISIALVEPGERDAS
                     AREARSFAGALEYAAARAQWRNEMLGVSADGRVLRYWRRDAANDRWVVLGDDDVLRAH
                     ALAAPVEGMALAYAGRSVASNAIVPLRASGRNEPFAFALATPRFRTVIALDPLNRASI
                     AGPDPNGS"
     gene            32607..32978
                     /gene="gspI"
                     /locus_tag="VFO53_08670"
     CDS             32607..32978
                     /gene="gspI"
                     /locus_tag="VFO53_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HET8876385.1"
                     /translation="MRRARAFTLIEVLVALAILAVALSAGMRAVAQSADGATSLKMRT
                     LALWVAQNRLAQAQLADPWPARGVASGDETQAGTRLAWRETVSDTPNPAFRRIEIVVS
                     EPGVPDYALARLVGYLGNAHR"
     gene            32965..33564
                     /gene="gspJ"
                     /locus_tag="VFO53_08675"
     CDS             32965..33564
                     /gene="gspJ"
                     /locus_tag="VFO53_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HET8876386.1"
                     /translation="MRIAERRARGFTLVEALVAVAILAIVATIAWRATAAMTDSEARL
                     ASESARWRRLDALMTRMEADMRKAIPRSARHGGIVEPAWLAGPADAAGNTALVFTRAG
                     PDAIDEPGSGGQRVGYRLRDGRIELLYWPRLDNVATRDPAAYALVDGVTRFHLLQLGA
                     DERWTDRWPPSGVDGVPRGVRVDIELADGSVVERWLALQ"
     gene            33561..34496
                     /gene="gspK"
                     /locus_tag="VFO53_08680"
     CDS             33561..34496
                     /gene="gspK"
                     /locus_tag="VFO53_08680"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HET8876387.1"
                     /translation="MKPASLSRPLASSVRQQGLALIIAMLIAALAAAVAVSVATAQSQ
                     WLAQVDHRRDQVEAQSIALAGVQWARAIVDADARGIDHLGEPWALPLPATPVENGEVE
                     GRIVDAQAMLNVNNLAGAQHGALERRRFTRLFATLGIPDTTLASIVDWVDADDVPQQG
                     GAEDAWYAGEADPSLAANGPATRIEELALVRTMTAQALARIVPFVTSLPPDTPVNVNT
                     AAPEVLAALLDGIDGGALAALVASRHDHPFASQEDFRGRLPTGVTVIDPDLIAVSTGY
                     FFVTVRARQGETIAQARALIHRDNASSAIVWQTIE"
     gene            34550..35749
                     /gene="gspL"
                     /locus_tag="VFO53_08685"
     CDS             34550..35749
                     /gene="gspL"
                     /locus_tag="VFO53_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HET8876388.1"
                     /translation="MLRVFLPAPPHIDRADQWVRYSADGRAIGRGRDVPSHWPADPVI
                     EAVLAACHVRLIALALPPMPRERLGGAARYALEDQIATSIDESSIALAEPRNGTCVAA
                     VASEALIRAVATQTRRIARIVPESALAPQGDGWVLWCASAGAGGFVRRADGSAFAVDA
                     SSDGALPGALAAALRAKGGPRTVHVAFSADAPQLAEWSRTSGVPFVGAPVWHWEQAPA
                     AAFASAPDFLRGTTPPADGASRRSILRSFRPALILAGAALAIHLAGLLVTWSWLNVEN
                     WRLSRAIVDEAAAAQLPDATTPQAAFDAIARRNAQRRHRALQAAPGDALPLLARAAPA
                     LGALPQQTLRSARYASGAWTLDLGKVDPALLSRVTGRLTAAGVEALAVPAAGGTRMRL
                     SLAAVAR"
     gene            35755..36237
                     /gene="gspM"
                     /locus_tag="VFO53_08690"
     CDS             35755..36237
                     /gene="gspM"
                     /locus_tag="VFO53_08690"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HET8876389.1"
                     /translation="MRWPRSITQAVQRADFSRRPAFAGLAIVAALVVAAVVALRLHDA
                     IARERENVTRNGLVLGAARARAAENATLARTNAVAKNVDLRASIEQVFARHALAYRVL
                     DAQESEGPLRIVIEAAPFDALVRALDALSREDGVRATDATLTARVDPGTVRAELELAR
                     "
     gene            36246..>36910
                     /gene="gspN"
                     /locus_tag="VFO53_08695"
     CDS             36246..>36910
                     /gene="gspN"
                     /locus_tag="VFO53_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HET8876390.1"
                     /translation="MRILLRLVPGLVVLAVALVVLAPAALLDAPLAARTGNRLHLVDT
                     RGVWWRGEGALATSDGAAHVPFAWKVALAPLLTGRLVAALRASDGAAGPTGTIAFADG
                     TIGVRDLHAVVPAALVPALAPSLKAVALRGDIELFAPSFTWRGTTGSGTVDATWAHAR
                     VIAGPWAVDFGRVIAKGVPAGDGIAGTVQASGGDVTIEGTLDTRAGATSASLELAPAP
                     GAS"
BASE COUNT         5566 a        12662 c        12431 g         6251 t
ORIGIN      
        1 gcatgcgctg cgtcgccatc ggcgagtgcg gccacgcgcg gccgaaagcg ccggcacagc
       61 ccgagaagct tcgcatgatt gcgattcgcg gcgagtgccg cgacgctgaa gcgctcgggg
      121 tgccgggcga cgacgtcgag cgtcgaatcg ccgatcgagc cggtagcgcc gagaagcgcg
      181 agcgtacggt gcatcacgcc gctccgagaa agaccgttgc cgcgagcgcg acgggcggca
      241 tcgcggcaag gagcgcatcg gtgcgatcga ggacgccacc gtgtccggga aggatcgcgc
      301 cgctgtcctt gacccccgcg cggcgcttca gaagcgattc gtgaagatcg ccgacgaccg
      361 acagtgcggc gagcgcgatg gcgaacgcga cccacgccac gattgcacgc gcatcgaagc
      421 caccctcgta tccggcgcgc cgcgcgagcg gcagcaacag cagcgcgtac acggcaacgg
      481 cgaggagcgc gccgtggacg ccctcccacg tcttgccggg actgatctgc ggagcaagct
      541 tgcgccgtcc gaagcggcga ccggcgaaat aggcggcggt atctgcaagc cagacgatcg
      601 ccatcgcagc aagtgcgagc cacggcgagc ggtcctgcag cgcgacgatg gcgacgaacg
      661 cgccctcgag catcacgaat gcggacaccg cgcgcacgag caccgaggca ggctgccagt
      721 tgcgcagcac ggagggcacg ccgacgacga gccagaacag tgtcgcggca ccgcaggcga
      781 gcgctacgac cgggtcgaag ccgcgcaatg cgcccaaccg cagcagtgcg caaccggcgg
      841 cgagaaccgc gatcacgaaa ccggtgcgtg cgccatcggc aaggccgatc agcttcgccc
      901 attcgctcgc cgcgacgagc acgatcaaaa ggacgacccc gccgaacacg agcgtgggca
      961 gcagcagcag cgctgccagg atggcggcga tgaggacgat gccggtggcg atacgcgtgg
     1021 ccagagcgga agccaaggcg gtccttgcaa atggctcgat ggcaacgggc gccacgcgtg
     1081 agcggccgct aggcgtcctg cgcctgcacg cgctggggca gccgtgcctc gatctgcgca
     1141 tcgttttgcg cctgcacctg ctcgctcgtg cgcccgaagc ggcgttcacg cccggcgtac
     1201 gacgcgatgg cggctgcgag cgcgctcgcg tcgaaatcgg gccacagcag atcggtgaaa
     1261 tagagctccg tataggcgag ctgccacagc aggaagttgg agatccgctt ctcgccgccc
     1321 gtgcggatga agaggtcggg ctcgggtgcg tacgccatcg cgagataagg ctcaagcact
     1381 tcgggcacga aggcgcgcgc gctcgccggc gcgtcggcat tcgcggcgaa atagcgccgc
     1441 gccgcctgcg cgatgtccca gcggccgccg taattcgccg caacggtcag cgtgagccgc
     1501 gtgttgttcg cggtgaggct ttcgccggcc gcgaccaggt cgcgaatgcg cgggtcgaag
     1561 cgcgacgtgt cgccgaccac cttgaagcgg atgccgttct tgtgcagctt cgcgacttcg
     1621 gcctcgagcg cgcgcgtgaa cagctccatc aggatcgaga cttcctcggc ggggcggcgc
     1681 cagttctcgc tcgagaacgc gaacagcgtc aggtattcga caccgtgctc gatcgaggcg
     1741 cgcacggcag cgcggacggc ctcgacgccc ttgcggtggc cggccacgcg cggcagcagg
     1801 cgccggcttg cccagcggcc gttgccgtcc atgatgatgg cgacgtgccg cggcacgtcg
     1861 cgcgactcgg gaatgatgcg ggtggagctc gtgaacatcg ttagcgatcg aaggttgggc
     1921 gaaatccgtg catcagatgg ccatcaggtc ggcttccttg gcctgcagca accggtcgac
     1981 gtcggcgacg aagcgatcgg tcagcttctg cagctcgtcg tgcgcgcgcc gctcgtcgtc
     2041 ctcggagcac tgcttgtcct tgaggagctt cttcagctgc tcgttcgcgt cgcgacgcac
     2101 gttgcgcacg gcgatcttcg cggcctcggc ctccttgtgg acgaccttga tcaattcctt
     2161 gcgccgctcc tcggtgagtg ccggcatcgg cacgcggatc atctcccccg acgtcgccgg
     2221 attgagaccg agatcggaat cccggatcgc cttttcgacg gtcgggatca tcttcttttc
     2281 ccatggcgtg acgccgatgg tgcgcgggtc ggcgagcgtg acgttggcga cctgcgaaag
     2341 cggcatcgac gagccgtagt aatcgacgtg gatgtggtcg agcaggccgg tatgcgcgcg
     2401 gcccgtgcgc accttggcga gatcggcctt caacgtctcg atcgaccgcg ccatcttctg
     2461 ttcggcggag ttcctgacgt cgctgatcat gaaacctcca tcatggggtc agataccaat
     2521 ttcccgaacg ccaccggcgt gcgccgtcgc agcgcaccag gggaaattgg tgtctgacct
     2581 cctccaactc taacaatgca ccagcgtccc ctcgtccccg cccatcacca cgcgcttgag
     2641 cgcaccgggc acgaagatcg agaagacctt cagcagcatg ttctggtcgc ggcacagcgc
     2701 aagcgcggtc gcgtccatca ccttgagatt cctgacgatc gcctcgtcga acgtgagcgt
     2761 cgagtagcgc cgcgcggtct cgtccttcag cggatcggac gtgtagacgc cgtcgacctt
     2821 cgtcgccttc agcacgatgt cgacgcccat ctcgcgcccg cgcagcgccg ccgccgtgtc
     2881 ggtggtgaag aacgggttgc cggtgccggc ggcgaagatg acgaccttgt gctcttcgag
     2941 atgccgcagc gcgcgcccgc ggatataagg ctcggcgacc tggtcgatgc gaagcgccga
     3001 ctgcacgcgt gactcgacgc cggcgcgccg cagcgcgtcc tgcaacgcga gcgcgttcat
     3061 cagcgtggcg agcatgccca tgtagtcggc cgttgcgcgg tccatgccgg cggcaccggg
     3121 tgcgacgccg cgaaagatgt tgccgccgcc gatcacgatg gcgacctgca cgccgagacg
     3181 aacgacctcc gcgacctcgg cgacgatccg atcgatcgtc tcgcgctcga tgccgtagct
     3241 gacgctgccc atcagcgctt cgccggacag cttgagcaga atgcgccggt aggccggcgc
     3301 gggcgccgcg gcgattccgg gaagcggcgc gatggccgcc gtgaccgcac tgtcgggtgg
     3361 gctcatcgag ggctccgcgg gtgccgtggt gacgcgggtc agctgcgggt catcgcggcg
     3421 acttcggcgg caaagtcgtc gttgcgcttc tcgatgcctt cgcccacgac gtagagaatg
     3481 aagccgttga cgcgcgcccc ttgcgcctgc acgagcctgc ccacggtctc cttgtcgttt
     3541 ttcacgaacg gctggggcag caacgtgacc tccgcgagga acttggcgac tgcaccttct
     3601 accatttttt cgacgatgtt ggcaggcttc cccgactcgg cggcgcgcgc tgccgcgatc
     3661 gagcgttcct tctcgacgac ggcgcccggc acctgctcgc gggaaacggc gatcggcttg
     3721 gtcgcggcaa tatgcatcgc gaggtccttg ccgagcgcct cgttgccgcc ggtgtagtcg
     3781 acaagcacgc cgatcctggt gccgtgcacg tacgacgcga cgcggccctc ggcggcaagc
     3841 cggacgaagc ggcgcaacgc gatgttctcg ccgatcttct gcaccatcgc gatgcggcgc
     3901 gcctcgaccg tgtcgcccga ggcgagcgtg cgcccggcga gcgcgtcgag atcggccggc
     3961 tgctcgcgca cgacgatccg cgcgacctcg ttcgcgaacg cgcgaaactc gtcgttcctg
     4021 gcgacgaaat cggtctcgca gttgacttcg accatcgccg ccgttcttgc gtcggccgca
     4081 accgcgatgc cgacgacgcc ctctgcggcg acccgcgatg ccgccttgct tgccttcgcg
     4141 ccgctgcgaa tgcgcaacag ctcctcggcc ttgccgaggt caccggcggc ttcggtcagc
     4201 gccttcttgc attccatcat gcccagcccg gtgcgctggc gcagttccat caccctgctt
     4261 gcggaaattt ccggcattct catgctccat cgttcattgc gctcgaaacg gcgtgcgttt
     4321 cgacggtgcc gcatgcgctc gtgacgcagc gggtcggccg gcgaggcgcg tgcagcacct
     4381 cgtcccgatg cagacgctca ggccgttgcc tcgtcgacga actcgtcgcc ggtggcgacg
     4441 atctcctgga tcgtgctggt cttgccttcg agcaccgagt cggcgacgcc gcgcgcgtag
     4501 aggcggatcg cccggctgga atcgtcgttg cccgggatca cgtagtcgat cggatcggga
     4561 ttgtggttgg tgtcgacgac cgcgaccagc gggatgccga gcttcttcgc ctcggtgaca
     4621 gcgatcttgt gatagccgat gtcgatcacg aacacggcgt cgggcagcgc attcatgtcc
     4681 ttgatgccgc cgaggctctt ctcgagcttg tcgagctcgc gctggtgcga aagcgcctcg
     4741 cgcttcgaca tgcggtcgaa tgtgccgtcc tgcttcatcg tctcgagatc cttgagccgc
     4801 ttgatcgact gcttgaccgt cttgaagttc gacagcatgc cgccgagcca gcgatggtcg
     4861 acatagggca tcgcgcagcg ctgcgcctcc tcggcgatga tctcgcgcgc ctggcgcttg
     4921 gtgcccacga agaggatcgt gccgcgattc tgtgcgagct ggcgcacgta tttcatcgcg
     4981 tcgttgtaca tcgccagcgt cttttcgagg ttgacgatgt ggatcttgtt gcgctggccg
     5041 aagatgtagt cggccatctt cgggttccag tagcgggtct ggtggccgaa gtgaacgccg
     5101 gcctccagca tttgacgcat ggttacggac atgctgtact cctctgtcag ggttgacctc
     5161 caccggcgga ttgcgctcgc gccgcgtgca tttcgcggct gacgacaccc tgaaacgccg
     5221 gtgtgcgatg tggaagacgc ggccgcttcg cctgcgtctg cccgggattg cgccgtttcg
     5281 ccgcctcccg attcgcggaa cgattcggga gtgcgtgcgt gagggcgcat gtgaccggcg
     5341 tcgcgcgtca ctttcaatcg acgtaaacca cggcgccggc aactcgttat tctagcatgc
     5401 ggaacgtact cgccggcacc ccggcacggc gccgcgccgg gcccgccgca acgcttgctc
     5461 ccaccccacc tgacgccatg acaccgcccg cacctgccgc atgcaaccat accggttgga
     5521 gcgtgctgcg cgtatccggc gacgatgcgc tcgcgttcct gcatgcgcag ttgtcgagcg
     5581 acgtgcaggc gctcgcgccg ggcgaaggcc agtactggtg ctacaactcg ccgaagggcc
     5641 gcatgcttgc gaacggcgtg ctgtggcgag cgccggccga cgtgccggac gcggcgatca
     5701 ctctcctcct cgccggcgat gtcgccggcg cgatccggcg gcgcctcgcg atgttcgtgc
     5761 tgcgcgcgaa ggtgcgcctc gatgacgcga gcgcaacgtc gatgctgatc ggcatcgcgg
     5821 gtgacggaag cgccgacgcg tcgaggcagg cgttcggcgt gtcgcctccg cccggccgcg
     5881 cggttgcgtt cgcgggcggc gcgagcgcgt tcacgctgcc cgatcgacgc atcgccatcg
     5941 tggcgccgca ggcggaacgc cccgtgctgc acgccgcgct cgcgcggcat gcgagtgtgg
     6001 tcaccaacga cgtgtggcgc tggttcggcg tcgcggcggg cgtcccgtgg atcacggcgg
     6061 cgacatccga tcttttcgtg ccgcagatgg cgaactggga cctgctcggc ggcgtcagct
     6121 tccgcaaggg ctgctatccg ggacaggaaa tcgtcgcgcg aatgcaatat ctcggcaggc
     6181 tcaaggaacg tcttttcgcc ttccacgccg atgcgctcga cgcggtgccg ggaacgcggc
     6241 tctactcggc cgcattcggg gcggaacagg cgtgcggaac ggtcgtcaac gcggcgcccg
     6301 atctcgtcgc cggtggcgtc gcgctgctcg cggtcgtgca actcgccgcc gtcgatgcaa
     6361 atgacgtcgc actgggcgaa ccgggcggcg cccgcctcgt gcggcgaggc ctgccgtacg
     6421 acgtgcccgc cggcgcgccc ggcgccgcac cgcgcccgag cggctgaatc atgtgcctcg
     6481 ccgtcatcgc gctcgatgcg catccgcgat acgccgtcgt catcgcggcc aatcgcgacg
     6541 agttccacgc gcgtccttcg gcgcccgcgc actggtggac cggcgctcat ggcaacgcgc
     6601 ttctcgccgg gcgcgacctc gagcatgggg gaacatggct cggtgtcgca cgcaacggcc
     6661 gcttcgcgtt cgtcaccaac gtgcgcgagc cgggccgcca cgatccgggc gctccctcgc
     6721 gtggcgtgct cgtgcccgcg ctccttgccg atccgcgccc gatcggcgtc gcgttgcagg
     6781 agctgatcgc cgacgcgcgc ggctataacg gcttcaacct cgtcgcggcc gacactgcgg
     6841 gcgcggcctt cggctccaac cgaggcgcgc acaccgtccc gctcggacgc ggactgcatg
     6901 gcgtctccaa cgccggactc gacacgccgt ggccgaagct cgtccgtgcg aaggccggcc
     6961 ttgccgcatg gattgcggcg gacgacgaca cgcccgatcc actatggcaa gtgctggccg
     7021 acccgacacc cgccgcggac gacgaactgc cggacacggg gttgccgcgc gagcgggagc
     7081 gccttttgtc gtcgccgttc atcatgagcg aaacctacgg cacccgctgc tcgaccctcg
     7141 tgaccatagc gcgcgacggg caggtggagt tcagcgagcg cagcttcgat gccgcgggca
     7201 cgacgcgcgg cgaggctcgc ttccactttc gcctcgatca gagtgcgcgc tccatcacgc
     7261 aatgcgcgat gccggcttcg tcgaacacgt cgccgcgggc gacgaacccg taacgcgcgt
     7321 aaaacggcat tgcggccacc tgcgcgttca gcatcacgcg cggcgcgccg cgtgcacgcg
     7381 ccgcgtcgac gagccgttgc aacagcgcgc tcccgacacc gcagccgcgc cacggcgcga
     7441 gcacggccac ccgcccgata tgcccgtcgg gcaggagccg cgcgcagccg atcggctcgc
     7501 ccgacgtgtc cgtcgcgagc gcatgcagcg acgtggcgtc ggcagcgtcc cattcgagcg
     7561 actcgggcac ccgctgctcg acgacgaaca catcgtggcg caccttgcgc aacgcgtccg
     7621 ccgcgtcggt ccagccgcag atgccaacgg tgaatcgctt catcggtacc gcgaaagaac
     7681 gagccgcgcg cgtccggcat cgacgtcgag cgcgcaatga ccgcccacgg gcagcgtcag
     7741 cttgtcgggc acgtgcccga acggcagccc cgcatgcacc ggcacgctga cgcgcgagcg
     7801 aagctgcgcg attgccgcgt cgagatcgta gcccgcgtcg ttcgcgtgca actggtattc
     7861 ggtgaatgcg ccgagcagca cggcgcgctg ccgcgcgagc acgcccgcat ggagcagttg
     7921 atgaaacatc cgctcgatcc ggtacggatg ctcgccgacg tcctcgagga acaggatgcc
     7981 gccgtcgatc cggggaaagt aaggcgtgcc gacgaggtgc gcgagcatcg cgaggttgcc
     8041 gccccacagc gtgcctgcgc acgaatgcga cgggccgtcg agcgcgcagt cgatcgacca
     8101 ttcgtcgttc ccgagcacgc cgaagcagtt gcgcagcgtg aacgccgacg gcgtcacggc
     8161 gccgaaatcg ctggcggcca tcgggccggc atacgtgacg atgcccgatc gcgcgagtgc
     8221 ggcaagctga aacgccgtga agtcggagtg tcccatccag cgcttcccgg cgcgcgcgag
     8281 cgccgcgaag tcgatgcggt cgatcaggcg gctgaagccg tacccgccgc gcagcatgac
     8341 ggcaacgtcg gtccgcggat cggccgcgac gcgctcgacc gcggcgagac gctcgtcgtc
     8401 cggcgccgag aatcgctgcc atcgtccgcg cgccgtcgca tcgctttgca cgcgatggcc
     8461 gaggcgtgca agcagcgctt ccgcgcggtc gacggcggca gggtcggtgg cgaaacccgc
     8521 cggcgccacg atcgtgatgt cgaggcttgt gctcatccgg tggcgcgttg ccggcgcgcg
     8581 gtgaagaacc gtgtgagaag tcgcgcgcac tcgtcggcgg cgacaccgtc ggtgacccgc
     8641 gtgtggtgat tgaggcgcgg ctcggcgaac aggtcgacca ccgagccgca ggcaccggtc
     8701 ttcggatcgc gcgcgccgaa aacgagccgc gcaacgcgcg catgcatgat cgcgccggca
     8761 cacatcgcgc agggttcgag cgtcgcgtag agcgtgcagc cggggagccg gtaattgccg
     8821 agcgccgcgg cggcttcgcg cagtgcagcg atctcggcgt gcgccgtcgg gtcgttcccg
     8881 gcgatcggcg cattgccgcc gcgcccgaca atggcgccgt cgcaaacgac cacggcgccg
     8941 accggcacct cgccgcgtgc ctccgcttcg gcggcgagcg tcagcgcatg cgccatccag
     9001 tcgtcgtcag tcgcgggcat cctcgatatg gaaacgatgc agcagccggt ggatgaccgg
     9061 agcgaacatg aggccggtgg acgcgagcag gatcacgccg cagaagagcg cgtagacgcc
     9121 gtcgaagatc tttcctgcgt cggatttcat gtcgacgacg ggtcccatgc cggacagcag
     9181 catcgccgac tgatgaaacg catcgagcca gctcaggtgc tcgagagcgc gatagccgat
     9241 catgccgacg gcgagcgtga tcacgacgag cccgagcgcg taggttgccg cacgcctgat
     9301 ccgggacagg aaggtcgtgc gcgatgcgag agcgccggcc aagggccgtg caggcggggg
     9361 cgcgacctgg ccctgcgcgt tgcgcgccgt cctgccgcgc gcggtcactg gaccactctc
     9421 cgcacttcgt gctgcggggc cgattgcaaa aaggccggct tcattcccat tcgatcgtcg
     9481 ccggcggctt gctcgacacg tcgtagacga cgcggttgat cccgcgcacc tcgttggtga
     9541 ttcgattgct gatgcgagcg agcagcgcgt gcggcagcgg cgcccagtcg gccgtcatga
     9601 aatcggtcgt ctgcacggcg cgcaatgcaa tcgcgtgctc gtacgtgcgc ccgtcgccca
     9661 tgacgccgac ggagcgcacc ggcaggaata cggcaaaggc ctgcgacacg gcgtcgtacc
     9721 acgactgccc ggcgtcgtcg cgcgcggcac gcagttcgtc gatgaagatc gcgtcggccg
     9781 cgcgcaggat gtcggcgcgc tcgcgcgtca cctcgccggg aatgcgcacg ccgagcccgg
     9841 gcccggggaa gggatggcgg aacaccatgt cgcgcggcag gccgagctcg aggccaagct
     9901 tgcgcacctc gtccttgaat agctcgcgca gcggctcgag cagtttcaga tgcagcgttt
     9961 cgggcaggcc gccgacgttg tgatgcgact tgatcgtgtg cgcgctgctg cccttcgcgg
    10021 acgccgattc gatcacgtcc ggatagatcg tgccctgcgc gagccatttc gcatcggtga
    10081 gcttcgccgc ttcgcgctgg aacacgtcga cgaagagccg cccgatgatc ttgcgcttgg
    10141 cctcgggctc gtcgacgccg gcgagagccg cgaggaattg gttgctcgca tcgacgtgaa
    10201 ccacgcgcac gccgaggtgc atcgcgaacg tgtccagcac ctgctcggcc tcgttctggc
    10261 gcaagaggcc gtggtcgacg aacacgcaga cgaggcgatc gccgatcgcg cggtggagca
    10321 gcgccgccgc aaccgacgaa tcgacgccgc cggaaagccc gagcagcacc tcatcgccgc
    10381 ccaccttcgc ccggatggcg tcgaccgcct ccgccgcata tccgtgcatt gtccagtcgt
    10441 ggccgcagcc gcagatgtcg tgcgcgaaac gcgcaagcag cgacgcgccc tgcggcgtat
    10501 gcgtgacttc gggatggaac tgcacgccat agaagcgccg cgactcgtcg gccatcgcgg
    10561 cgatcgtgca cgcatccgac gagccggcga gcgtgaatcc cggcggcagg ccgacgacct
    10621 tgtcgccatg gctcatccac acgtcgagca gaccgtggcc atcggcattg cggcggtcct
    10681 cgatgtcgcg caacagcgtg gtgtgcccgc gcgcgcgcac ctcggcacgt ccgaattcgc
    10741 gcacgcgccc cgcttcgacc ttgccgccga gctgctcggc catcgtctgc atgccatagc
    10801 agatgccgag cacgggcacg ccgagcgtcc acaccgcggc cggtgcgcgg ggcgtctcac
    10861 cggtggtgac gctcgccggc ccccccgaca gcacgatgcc gcgcgcatcg aaagcacgca
    10921 cgaagtcatc accgacggtc cacggatgga tttcgcagta aacgtcggtt tcccggaggc
    10981 gccgcgcgat gagctgcgtg tattgcgcgc cgaaatcgag gatcaggatc cggcagtgcg
    11041 gaaaagtggg gccagagacg actttcgaac cctgacgatg caattcggcg gacggcgatg
    11101 cggaagtcgt ctctgacccc acttctttat tcgacgcggt aattcggcgc ctccttcacg
    11161 atctgcacgt cgtgcacgtg cgattcgcgt atgcctgccg acgtgatctc gacgaactcg
    11221 gcgcgcgtcg acaggtcgcg gatcgtgggg caaccgcagt agcccatcgc cgcgcgcagt
    11281 ccgcccatta gctggaggat cacgttggcg agcgatccct tgtacggaac gcggccctcg
    11341 atgccttcgg gcacgagctt ctccgccgcg ccctcgcctt cggcatcctg gaaatagcga
    11401 tcggaactgc cgagctgcat ggcgccgatc gagcccatgc cgcgatagct cttgtacgag
    11461 cggccctgat agagctcgat gtcgccgggc gattcctcgg tgccggcgaa aaggcttccg
    11521 agcatcacga ccgacgcacc ggccgcgatc gccttcgcga tgtcgcccga atagcgaatg
    11581 ccgccgtcgg cgatcaccgg cacgtcgccc gcgcccgaaa gtgcatcctg gatggccgac
    11641 acctgcggca cgcccacgcc ggcgacgata cgcgtcgtgc agatcgagcc ggggccgatg
    11701 ccgaccttca cgccatccgc gccatgctcg acgagcgcct tcgcgccgct tgcggtggcg
    11761 atgttgccgc cgatcacctg cacctgcgga taacgcttct tcaccgactg cacgcgatcg
    11821 agcacgccct gcgaatggcc atgcgacgtg tcgacgacga tcacgtcgac gcccgcagcg
    11881 acgagtgcct cgacgcgcgc gtcggtatcc tcgccggtgc cgaccgccgc accgacacgc
    11941 aggcgtccga gatcgtcctt gcaggcgatc ggatgctcgg tcgatttcaa gatgtccttg
    12001 acggtgatga gcccgcgcag ctcatgctcg cggttgacga cgagcacgcg ctcgaggcgg
    12061 tggcgatgca tcagcgcctt cgccgtctcg agatcggtac cctcgggaac cgtcacgagc
    12121 ttctcggcgc gcgtcatgat ctgcgacacc gcctgatcca ggttcgtctc gaagcggagg
    12181 tcgcggttcg tgacgatgcc gacgaccttc cttccgtcga cgaccggcag tcccgagatc
    12241 cggtactgat gcgtcagcgc cagcacctgg cgcaccgaca tcgacggcgg aatggtgatc
    12301 gggtccttca cgacgccgct ttcgaaccgc ttgaccttcg cgacctcggc cgcctggcgc
    12361 gcgggcgcca tgttcttgtg cacgatgccg agaccgcctt cctgcgcgat ggcaatcgcg
    12421 agccgcgcat cggtcaccgt gtccatcgcc gccgagagca gcgggatgtt gagcgaaatc
    12481 gtgcgcgtga ggcgggttac gagcgaaacg tcgcggggaa cgacgctgga atgggcggga
    12541 acgagcagga cgtcgtcgaa agtgagtgcc ttgcgcacga cacgcatcga actctccttg
    12601 tcgcaaagag cgattataac cgcgctcgcg tccgcccccg tgaccgtacc gatcccgcga
    12661 catgttcatg cctgcgcgag ccggtccacc gttggtttga ttgacggcga agtgccacgc
    12721 ggggtaactt gccaatgtca taaatgccgg ggtcgcccat gtcgcgcgca tcgctttacg
    12781 cgttcgccgt tctgttgctc gccgccggtc attcggcgag cggtgcggcc gcgctctaca
    12841 aatggaccga tgcgcaggga cgcatcgtct attccgacca gccgccgcag gcgaacgtca
    12901 agaccgagca gctccgaggc gcgccgccgc ccgccaaccc gaacgcggcg aaggagcttg
    12961 cgcagcgcga ggccgatttc aggaagcgcc aggccgatgc ggagaaggcc gcggccaagg
    13021 cgggcaagga gagcgcgagc agcgccgacc atgccgagag ctgcgcgcag gcgaaaggtc
    13081 agctgaagca gctcgcggag agccagcttg cgatctatcg ctacaacgag aagggcgaac
    13141 gcgaggtcat ggacgaggat gcgcgcggcc gcgagcgcgc gaagctcaac gcatggattc
    13201 gcgacaacaa gtgctgaggt cttactggtt ctgcttcttc tcgagcgccg cctggttgat
    13261 cttcacgtcg gtgtcggcct tgaggctcgc gagataggcg ttcatcagct cgcgtccgag
    13321 ctcgctgccg acgcgcgctg acgcggcgtc gaccttcgcc gcatcgggcg ccggtggatc
    13381 gatcaccttg tcgatccggt agatcgagaa tccgccgcgc tcgttggatg ctcccgcata
    13441 ctcgggcagc ttgcgctcgc cggcatcgaa gatcttcttc aggccatcgg ccgtgacgcc
    13501 gggctgcgcc tggttgcgcg tgagcgtcac cggcttgtcg aatgcgacgc ccgcgtcctt
    13561 gcccgcctcg agcagcgcga gcttctcgcg tccggtcttc actgcaagct cgcttgcccg
    13621 ctggtggatc agctgctggc ggatctcgtc cctgacttcg gcgaacggct tcggcgccgc
    13681 cggcttgtac tcgacgatgc gacccgccat caacgcgttg ggcccgacct cgatcgcttc
    13741 ggtgttgcgc ttgctctgga cggactcggg cgaaaagagc gcctgcacga atttcgcgct
    13801 gcccatcgcg atcgattgca tcttttcgcg cgtgaccagc ggcgtcgttt caaccttgag
    13861 gtcgagcgtc ttcgcggcac cggcaagcga atcggcctgc tcgtagacga gattctggaa
    13921 ttgatcggcg gcggaagcga atttctgcgc cgccttctgg cgcttcaact ccgcctcgat
    13981 gcgtcccttc acttcgtcga agctttgcac gtgcggcggc gtgatgccgg tcaccttgat
    14041 cacgtggtaa ccgaagtcgg tctgcaccgg cccgacgatg tcgccgacct tggctgcgaa
    14101 caccgcatcc tcgaacggct tcaccatcgc tccccgcgca aacgagccga gatcgccacc
    14161 ctgcgcggcg gagcccggat cctgggagtt ggccttcgcg agctcggcga acgaatcggg
    14221 cttcgcgcgc gccttctcgg cgagctcggc cgccttctgt ttcgccttgg ccttgtccgc
    14281 gtcgctcgca tccttcttta cggcgatcag gatgtgcgac gcctgccgct cctcgctcgt
    14341 ggtgtactgc ttcgcgttcg cctcgtacgc ctgcttgacc tcggccgggt cgaccttgac
    14401 ctgcgcggcc atggcatcct gcgtcagcag gatatagtcg accttcgcga cctcgggcgt
    14461 cgcgaacgcc ttcggattct tgtcgtagaa cgccttcacg tcgtcgtcgc tgaccttgac
    14521 cgacttctcg aacggctcgg cggcaacctg cgcgatggcg acgtcgcgct tctggtcgag
    14581 cagcgtcagg tagcgctgca ccgacgactt cgcggcgatg ctgccgccga tgatcgggtc
    14641 ctgcagcggt gacaggacga gatcgccgcg cactcgctgc tcgaacatca gcggcgacat
    14701 gttctggctc gcgagcacct cgcgataccg gtcggccgag aacttgccat cctcctggaa
    14761 cggcggcaag gccgcgatga actgcgcaag ctgcgcgtcg gtgacgcgaa agcggcccgc
    14821 gcgcgcctcg ttctccagca gccgctggtt cacgagctgg tcgaggagcg cgtagcgcat
    14881 ttcgggcgag tcgagcatcg ccgggtcgaa gttgctgccg agcgcctggc gcatccgctg
    14941 ctgctggtcg cgcagcgcat cgtcgaattc ggcctgggtg atcttgttct tgccgacgct
    15001 cgcgaccgtc tgcccgctgt cgccgccgcg gaagtaatag tcgacgccga agaacgcgaa
    15061 cggcagcgtg atgagcgcga gcacggcttg cgtgacacgc gggtgcttgt gcaggagatc
    15121 gaacatttgg gaattacctt tgcgatcgga cgttgcccga atgcggacaa gggcgaactc
    15181 gcgttcgccc ttgtcggtgt tggcggagtg gacgggactc gaacccgcga cccccggcgt
    15241 gacaggccgg tattctaacc aactgaacta ccactcccgg ataagtaggg tcagagacga
    15301 ctttcccgca ccgtctccgc gaaccgcagg ccgaggtcgg gaaagtcgtc tctgacccta
    15361 cttatccgca ccgtctccgc gaaccgcagg ccgaggtcgg gaaagtcgtc tctgacccta
    15421 cttatcgtgg tgggtgctga cgggatcgaa ccgccgacat tcgccttgta agggcgacgc
    15481 tctaccagct gagctaagca cccctgatct accctagcga agcacgctag tttagcgcat
    15541 ccttcagcgc ctttccagcg cgaaacttcg gcaccttgcc gcccttgatg ctgattgcat
    15601 cgccggtgcg cgggttgcgg cccgcccgcg ggctgcgctt gccgacatag aacgagccga
    15661 atccgaccag cgtgacgatg tcgcccttgc gcagcgatgt cttgatcgcg ccgaccgtcg
    15721 catcgagcgc gcgtcccgcg gccgccttgg agatgtcggc atgacgcgca atcgcgtcga
    15781 tcatttcgga tttgttcaca aagcgtcctt tcgataggga atgcgacgcg cgaggtgagc
    15841 acttcggaag tcttgaagct gccggcgcat gacgatcgct gcgcggaacg atctttatag
    15901 cgctcgcgtt ttccaggtgt caaggctcgc gcgacatacg tgatcgaata aaaaaggcgg
    15961 acgatcggat cgtccgcctt cgtgttgcac ctgcggcgcg atcaatgctt gatgaccgcg
    16021 ctcttgtcgt ctgccgccgg cgtggcggga gggacggccg cctgcgcctt gtcggcgtcg
    16081 tcggtgatcg gcgtcggctg ccgttcgagc gcacgctcca gcacctgctc gatccagcgc
    16141 accgggtgga tgtcgagacg attcttgatt tcgtcgggaa tctcgacgag atccttcacg
    16201 ttctcttccg ggatcagcac ggtcttgatg ccgccccggc ccgccgccag cagcttctcc
    16261 ttcaagcccc cgatcggcag cacttcgccg cgcagcgtga tttcgccggt catcgcgacg
    16321 tcgcagcgca ccggaatgcc ggtcagcgtc gacaccatcg atgtcgcgat cgccgtgcct
    16381 gccgacggcc cgtcctttgg cgtggcgccc tcgggcaggt ggatgtgcag gtccgtcttc
    16441 gtgtagaact ccggcgcgat gccgagctcc ttcgaccggt gccgcaccac cgacaaggcc
    16501 gcggcgatcg actcgttcat cacgtcgccg agcttgccgg tggatgtcgt cttgcccttg
    16561 ccgggaagcg cgaccgattc gatcgtgagc agatcgccgc cgacttcggt ccacgcgagg
    16621 ccggtgacct ggcccacctg gttgcgccgc tcggcgatac cgtacgtgta gcggcgcacg
    16681 ccgaggtact tgtcgaggtt cttcgagctc acctccatcg gcccgcgcgc cttgcgcgcc
    16741 ttgccttcgc tttgcttgag cagcagcgcc ttcacgacct tgcggcagat cttgctgatg
    16801 tcgcgctcga ggttgcgcac gcccgattcg cgcgtgtagt agcgcacgat gtcgcgcagc
    16861 gcgctttcgg cgacgtgcag ctcctcgggc ttcagtccgt tgtgcttgat ctgcttcggc
    16921 agcaggtact gcttggcgat ggcgatcttc tcgtcctcgg tgtagccgga gaggcgaatc
    16981 acctccatcc ggtcgagcag cggcgccggg atgttcatcg tgttggcggt ggcgacgaac
    17041 atcacgtcgg acaggtcgta ctcgacctcg acgtagtggt cgacgaacgt gttgttctgc
    17101 tcgggatcga gcacctcgag cagcgccgat gacggatcgc cgcggaagtc ctgtcccatc
    17161 ttgtccactt cgtcgagcag gaacagcgga ttcctcaccg cgaccttcgt catgttctgc
    17221 agaatcttgc cgggcatcga gccgatgtac gtgcggcggt ggccgcggat ctcggcctcg
    17281 tcgcgcacgc cgcccaagct catgcgcacg aacttgcgtc cggtcgcctt ggcgaccgac
    17341 tggccgagcg aggtcttgcc cacgccgggc ggcccgacga ggcacaggat cggcgccttc
    17401 agccgatcga cgcgctgctg caccgcgagg tactcgacga tgcgttcctt gaccttctcg
    17461 aggccgtagt gatcggcgtc gagcaccttg accgcgtgcg cgaggtcctt gttgatcttg
    17521 ctcttcttgc gccacggcag gccgagcagc gtgtcgatga aattgcgcac gacggtcgct
    17581 tcggccgaca tcggcgacat catccgcagc ttcttgagct cggtcatcgc cttgttctcg
    17641 gcctcgcgcg acatcttcgc cgcacggatc ttcttttcca tctcctcgag gtcggcgttc
    17701 tcgtcgccct cgccgagttc cttctgaatg gccttgacct gctcgttcag gtagtactcg
    17761 cgctggctct tctccatctg gcgcttgacg cggccgcgga tgcgcttctc gacctgcagg
    17821 atgtcgacct cgccttcgag cacgccaagc aggtgctcga ggcgcttgcc gacatcgctc
    17881 atctcgagga tctgctgctt ctgctcgagc ttgagcggca ggtgcgcggc gatggcgtcg
    17941 gcgagacggc cgccgtcgtc gatgccggac atcgacgtca ggatctccgg cggaatcttc
    18001 ttgttgagct tgacgtacga atcgaactgc gtaaggagcg cacggcgcat cgcctcgacc
    18061 tcatgcgcgg tgcccgggtc gcccgggatc ggctgcgtgt gcgcggcaag gtactcgcca
    18121 tgctcctcga tgctgcggat atgtgcgcgc tgcgtgccct cgacgaggac cttcacggtg
    18181 ccgtcgggga gcttcagcat ctgcagtacc gtcgcgacgc tgccgattcc gtacagatcg
    18241 tccgccgcgg gatcgtcctt ggccgcctgc ttctgcgcga caagcaggat gtgcttgccg
    18301 gcgtccatcg ccacgtcgag cgccttgatc gacttcgggc ggccgacgaa cagcgggatc
    18361 accatatgcg ggaagacgac gacgtcgcgc agcggaagta gcggcagcgt ggtgacgccg
    18421 gtcgggaggg atgtatcgtt gggcattcgt gcctcgggtg ggagagtatc ggtcaacatg
    18481 gggccgtcgg gggccccttc aaggaacagg gtacccgctg cggcccacgg gtcgcccatg
    18541 gggttgacat tcctacaaac ggcacgctgc gcgccatcgg ccccggggct taggcaccgt
    18601 gggctcggat gaattcctgc cacgcctcgc cggcgggcgg accgcggcga gacgcgctcg
    18661 atcgatcgct aaccgtttga tcctgcgaag ggttccggtc gcgcgaattg aacgtcgacg
    18721 cagtcgacgg tcgaatgagc gcagttattc cggcgcgccg acggtcagct caccgaaggc
    18781 acgctcgcga ccggcgcgac ggaataacgc tacttggccg atgccgccac tttggccgtc
    18841 tcggcgaaca tcaggagcgg cttgccgtcg ttggcgatcg tcgactcgtc gacgaccacg
    18901 cgcgcgaggt tggacatcga cggcaggtcg tacatgacgt cgagcagcac ctgctcgagg
    18961 atcgagcgca gcccccgcgc gccggtcttg cgcgccagcg ccttgcgggc gatggcacgc
    19021 agtgccagct cgcggatttc gagctcgacg ccttccatcc ggaacatctt gtggtactgc
    19081 ttgatcagcg cgttcttcgg ctcgacgagg atctggatca gcgccgcctc gtcgagctcg
    19141 tcgagcgtgg ccacgaccgg caggcggccg acgaattcgg gaatcagccc gaacttgatc
    19201 aggtcctcgg gctcggtgcc gcgcagcagg atggtcatgc tgcgccgatc gtcgggcgag
    19261 gccacctcgg cgccgaagcc gatgccggtt ttcgcggtgc gctggcggat gatcttctcg
    19321 aggccgtcga acgcgccgcc gcagacgaac aggatgttgg tcgtgtcgac ctgcacgaac
    19381 tcctggttcg gatgcttgcg cccgccctgc ggtggcacgc tggcgatcgt gccctcgatc
    19441 agcttcaaca gcgcctgctg cacgccctca cccgagacgt cgcgcgtgat cgacgggttg
    19501 tcgctcttgc gcgagatctt gtcgatttcg tcgatataga cgatgccgcg ctgcgccttc
    19561 tcgatgtcgt aatcgcactt ctgcaacagc ttctggatga tgttctcgac gtcctcgccg
    19621 acatagccgg cctcggtgag ggtcgtggcg tcggcgatga cgaacggcac gtcgagcagc
    19681 cgcgcgagcg tctgcgccag cagcgtcttg ccggagccgg tgggaccgat cagcaggatg
    19741 ttcgacttgg caagctcgac ctcgtcgtcc ttggcgccgg attcgagtcg cttgtaatgg
    19801 ttatagacgg cgaccgagag gattttcttc gccggctcct ggccgatcac gtactggtcg
    19861 aggatctggc ggatttcatg cggggtaggc agcttgctgc gatcggcgcg cgcggtttcg
    19921 ctgccggtgc cctcctcgcg gatgatgtcg ttgcaaagct cgatgcactc gtcgcagatg
    19981 aacaccgacg ggcccgcgat cagcttgcgg acctcgtgct ggctcttgcc gcagaacgag
    20041 cagtacagga gcttgtcgcc ggatgaggat ttttcggcca ttaacgcagc acctcgtgaa
    20101 ttcgattgac tttactcggg taagagtcgc ttttgcaaca ctttatcgac gattccatag
    20161 tcgcgcgcct gctcggcggt gaggaagttg tcgcgctcgg tgtcacgcgc gatctgctcg
    20221 accggcttgc ccgtatgcat ggccatcagc acgttcaggc gctggcgcag gctgaggatg
    20281 tacttggcat ggatttcgac gtccgaggcc tggccctgga agccgccgag cggctggtga
    20341 atcatcacgt ccgaattggg cagcgcgaaa cgcttcccct tcgcccctgc cgcaagcaag
    20401 aacgcgccca tcgacgcggc catcccgacg cacaacgtgg acacgtcggg cttgatgaac
    20461 tgcatggtgt cgtatacagc gaacccggct gcgatcgagc cgccgggcga gttgatgtag
    20521 aagtgaatgt ccttgtcggg gttctcggat tcgagaaaca gcatctgcgc gacgacgagg
    20581 ttggccgtcg actcgttgac gggtccgacc aggaacacga cgcgttcctt caacaggcgc
    20641 gagtagatgt cgtacgcgcg ctcgccgcgt ccgctctgct cgatcaccat cgggatcagg
    20701 cccaggcctc ttggttcttc catgtggatt cgatgctttc gttttgagtg tcaaccggcg
    20761 gagccgcgcg gccattccgc ggaaccgtac cgtggaacga cgaagatggg tcttcgacgc
    20821 ctgatcgtca agcgccgccc ggtgaacgga accgctcagg cgccagtctg cggcgccatc
    20881 acctgctcga aggtcgccgg cgcgtcggtg actttcgcgc gtgcgagcac ccagtcgacg
    20941 acgttctgct cgagcaccat tgcctcgaaa tcgcgcagcc gctcgggctt ttcgtaatgc
    21001 caacggacca cggcgtcggg ctgctcgtaa gtctgcgccg tttcggtcac cagcgcgcgc
    21061 acctgctcgg gcttcgcgtg caggttctcg ctacgcacga gctcgccgat gatgaggccg
    21121 agcgcgaccc gctcctcggc ctgcgggcgg aacaggtcga ccgacacgtc gaggccttcc
    21181 gctttgacgt tctgctgctc gcggaggttc gcgagcgcgc gctcgcgcag cagttgcgct
    21241 tcgctttcga cgagcgcctt cgggatggcg aagctcgcct gcttgcgcaa gccttgcaat
    21301 gcctgtgcct tgatcgtcga ttcgatcttg cgcttcaact cgagcttgag attggcctcg
    21361 acctcacgcc gcaggtcgtc ggtctcgccg ctcgcgatgc cgaacgcgcc ggcgaaatcg
    21421 gcgtcgagcg gcggcaggtg cggctcctcg acccccgcga cgcggagcga gaagtgcgcc
    21481 gtcttgcccg ccacttcgcg gccgtgatag tcggccggaa acgtgaggtc gaactccacc
    21541 gtttcgcccg cgctcatccc ggttaccgcg gcctcgaact cgggcagcat gcgcgcttcg
    21601 ccgagaacga tcggaaaatt cttcgcctgc ccgcccggga actcgacgcc gtcgatcgtg
    21661 ccggtgaaat cgacctgcac gcggtcgccg ccttgcgcgg cgcgctcgac cggcgtgtag
    21721 gtcgtgcgct gcttgcgcag cacgccgagc gtgcgctcga catccgccgg cgtcacctct
    21781 gcgcgtggcc gcgacacctc gatcgtcgcg acgtcggcga cctttacttc cggataaacc
    21841 tcgaagaccg cggagaactg gagcgcgtcg ggggccggcg cgtcgccgct acgcggctcg
    21901 atgcgcggca cacccgcgac gcgcaggttc tgtgcgcgca ccgcgtcgtt gaacgtggac
    21961 tggacgcgct cggtgatgac gtcggagcgc acctgcggcc cgtactgctg gtcgagcatg
    22021 cgcagcggca ccttgcccgg gcggaagccg gcgaccttcg ccgtgcgcgc gagccgcgac
    22081 agccgcttct tcacctcgcc ttcgatatcg gcgaggggta cggcaacatt caagcggcgt
    22141 tcgagcgcgc cgagcgtttc gagtgtggtt tgcatgcgag aaggaatccg atctggtgcg
    22201 aaagggggga ctcgaacccc cacgggctgc cccgccagct cctaaggctg gtgcgtctac
    22261 caattccgcc actttcgcga cttcggacgg ccgcgagaag agaatcggct agtatagccg
    22321 accgggctcg cgctcgccag acagcttacg ctgcttcgcg cccgcctcgt tcgcgtttac
    22381 cggatcgtcg cttgtcggtc ggccattacg aaaacttccc cgttgcgtcc gtgctcgtgc
    22441 cggcgcgtta ccgtgatgcg gtcatcgcga tctatcgctt cgcgcgcacg gccgacgaca
    22501 tcgccgacga aggcggcgcg gcgcccgccg accggcaccg cgcactcgac gcgttttcgc
    22561 gtcggctcga cgccatcgag cgtggcgaaa cacccgccga gccgccgttc gccgatctcg
    22621 cgctcgccgt gcagcggcat cagctgccga tcgcccgctt gcgcgacctc ctgtcggcgt
    22681 tcgcccagga cgtcgacgtc acgcggtatc cgacctacga cgatgtgctg gactactgcc
    22741 gccgctcggc caatcccgtc gggcggctgc tgctcgcgct gtacgggatc gacgatgcgg
    22801 gagcgctcgc cgagagcgac gcgatctgca cggcgctgca actcgcgaac ttctggcagg
    22861 acgtcgccat cgactggcgc aaggacacgc gactctacct gccgctcgag gatctcgcgt
    22921 gcttcggcgt gccgctttcg cacgttgcga acggcgtttg cgatgcgcga ttttcgcggc
    22981 tgatggcgtt cgagaccgcc cgcacgcggg cgctgttcct gtccggccgg ccattgatcc
    23041 ggcgcctgcc gtggcgcctc ggactcgagc tcaccgcggt catcgccggc ggcatgcgcg
    23101 tcctcgagcg catcgacgcc gtgcgcggcg acgttttcac gcagcgcccc gtcctcggca
    23161 agcgcgactg ggcgctcctc gcgctgcgca tgctcgcgcc gtcggcggca gtgcgctttg
    23221 catgacgccc aacgaatact gcgcgcagaa ggtcgcgcaa agcggctcga gcttctacta
    23281 cagcttccgg ctgctgccgc cgccgcggcg cgccgccatc agcgcgctct atgcctattg
    23341 ccgcgaggtc gacgacgtcg tcgacgaggt gtcggatccg agcgtcgcgc gcgtgaagct
    23401 cgcctggtgg cgcaccgaag tcgacgcgat cttcacgggc acgccgcagc atccggtcgc
    23461 gctcgcgctt gcggacgcgg ccgagcgtta cggcctcgcc cagtcgaacc tgcaaacggt
    23521 gatcgacggc atgcagatgg acctcgagcg ggcccgctac gtcgacttcg ccgagctcga
    23581 gacgtattgc gatcgggtcg cgggcgtcgt gggtgtcatg tccgccgcga ttttcggcta
    23641 cgaagatcca tcgacgctcg tctacgcgcg aaggctcggc gtcgcgttcc agttgacgaa
    23701 catcatccgc gacgtcggcg aagacgcgcg ccgcggccgc atctacctgc cgcaggacga
    23761 gctcgcgcgc ttcggcgtcg cgccgtcgga tctgctccgc gcgcgctcgt cgccggcgtt
    23821 cgtcggcctg atgcgctttc agaccgaacg ggcgagcgcc tggtacgaca cggcgctcgc
    23881 agcgctgccc gagcgcgacc gccgtgcaca acgcgcaggc ctcgcgatgg cggccatcta
    23941 ccggacgctc ctcggcgaga tagcgcgcga cggctatcgg gtgctcgacc ggcgcatcgc
    24001 gctgacgccg ctgcgcaagc tgtggatcgc gacgcgggcc gcgtggcggg catgagcgcg
    24061 ctgaagtaag tccgatgagc gcgcgcgatc gtcgatcggt cgtcgtcatc ggcggaggct
    24121 gggcgggctg cgctgccgcc gtcgagctcg cacggtgcgg acattcggtg gaattgcacg
    24181 aagcggccat cgcgcttggt ggccgcgcgc gcgccgtcgt ccgcgacgga ttgccgctcg
    24241 acaacggcca gcatctgctg ctcggggcgt acaccgacac gctcgcgttc gcggccgcga
    24301 ttcgggatcg gacgtcgtcg gcatggacga tcgagccgct cgcgctgcgg ccgttcgctc
    24361 gcgcacagcg caacacgctg tcgctgtcga cccgccatgc gccggcgccg ctcgcgctgc
    24421 tggcgggact cgtccgcgcg cgcggcctgc cgatcggggc acgcatcgcc accattcgct
    24481 ggttcgttcg acaacggcgc aacggctggc aatgcgcgcc ggacctcacg gtcgccggat
    24541 tgctcgcgga tgccccggcc gtcgtgcgcg acgatctgtg gcatccgctc tgcctcgcgg
    24601 cgctcaacac gccgcccgcg cgcgcgtcgg cgcaggtatt cctcaacatc ctgcgcgaaa
    24661 cgttcggcgc cggcgcgcat gcaacgtcgc tcgtcctgcc gcgcgaaggc ctcgccgagg
    24721 cggtgcccga agcggcggcg cgctggctcg cggcgaacgg ccatccggtg cgccgctcga
    24781 cgcgaacgcg aatccggcgc atcgacgcgg atggcgtgct gctcgaagcg tcgggcgagc
    24841 atgcgcgagc cgatgcggtc atcgttgccg tcggtccgca tcaactcgcc tcggcgttcg
    24901 cgccgggcat cgagcgcgac cacccctcga tcgtgcaggc gctcgcttcc gttgcgcgct
    24961 tcgattacga gccgatcacg accgtgtatc tcggttacgc ggcggcccgt gcattgccgc
    25021 gcgggctgct gcgtctcgac gacgcgcccg gacagtggat cttcgatcgt tccgatattc
    25081 tcgaacgcgc ggcggcatcg gcgacgcgcg cgccgatgcg ctcgctcgtt tcggtcgtga
    25141 tcagtgcgcg cgggccgcac agcgcgctcg atcatgcggc gctggtcggc gccaccgatg
    25201 gacagcttcg ccgtcttgca ccggacctgg caccgctcgt gtggtcgcgg gtgatcgagg
    25261 agaagcgcgc gacgtatgcg tgcgtgcccg gtcttgcgcg acccgcgtgc ggccgtctcg
    25321 tcgaacgggt cggcctcgcg ggcgactatg cgtacgctgc gtttcccgcg acactcgagg
    25381 ccgccgtacg cagcggacgc atcgccgccc acgcgatcat tggtgccccg tcggcgcgat
    25441 cattggaccc ccacgcttgc ggctaaagcc gctgcgctgc ccccggcggg ggcgcagttc
    25501 gcgccttggg gcggcccggc ggcgctcacc cccggagcag ccgctgctgc tcgcggttcc
    25561 attcgcgctc cttgatcgcc gcgcgcttgt catgctgctt cttgcccttg gcgagcccga
    25621 cggcgagctt gacgcggccc ttggcgtaat gaaggtcgag cggcgtcagc gtgtaaccgg
    25681 cgcgctcgac cttgccgatc agccggttga tttcctcgcg gtgcatcagc aggcgccgcg
    25741 tgcgcgtggg atcgggcgtg acgtgcgtcg acgcggtcgt caacggcgcg atgtgcgcgc
    25801 cgatcagcat gagcccgccg tccttgacga cgacatacgc ctccttgaga ttcgcgcgac
    25861 cgccgcggat cgccttcact tcccagcctt cgagcgcaat cccggcctcg aagcgctcct
    25921 cgataaaata gtcgtgaaag gccttccggt tctcgacaat gctcattgca atgcccactg
    25981 acgcgccgcg ccgttacccg ctgtccgcgc attctacgtg caacgcagct gcacggccgc
    26041 gccgcacgcc tccgcgggac ccgtcatgcg cgcggtgaaa aagagcgtcc tcgtgcccta
    26101 ctctgccgcg cagatgttcg agctcgtcga ccgcgtcgag ctctatccgc agttcctgcc
    26161 ctggtgcggc ggcacgcagg tgctcgagca gggcgaaaac cgcaagaccg cccgcatcga
    26221 catcgactac cacggcgtgc gtgcgcactt caccaccggc aacgtgaacg atccgccgcg
    26281 ctcgatcgtg gtgacgctga aggatggccc gttccgtcat ttgcatggcg agtggcgctt
    26341 catcgcgctg tcggagcacg gatgcaaggt cgaattcgat ctcgcgtacg agttcgcgac
    26401 gagcctgctc gaccgcgtga tcggccccgt gttcagccac atcgcaaaca cgttcatcga
    26461 cgcgttcgtc aagcgcgccg aaagcgttgg ctaggatgct acgtctgccc acgcacgctg
    26521 cgccccgggg ggctcatcag tcgttcgggg cggccctcca cgactgacat gcgcgtgcag
    26581 gttgccttcg tcgccgatgc gcagcagacg ctcgcgacgc tcgatctgcc gcaaggctca
    26641 cgcgtcagcg acgcggttgc gcagtcgggt ctcgggctcg tcgacgacgt gaattgcgga
    26701 cgtcttgttt gcgcgatctt tggccgccgt gccgatcacg acacgccgct tcgcgaaggt
    26761 gatcgcgtcg agatcacgcg cccgctcgtg tgcgacccga agacggcccg gcaccggcgt
    26821 gccgcgcaag gccgctgacg cgctgcctac tcgcgtgcgc cgacgcggcg cagctccttc
    26881 accgtatcgc tctcgttgcc gcgcatcgcc cacgcaagtg cggtggcgcc gtcctggttg
    26941 cgcagtgaaa cgtcggcgcc gtgcgcgatg agcagccgcg ccgtgtcggg atgatgctcg
    27001 tggatggcca tcatcagcgc cgtggtgccg ttcggcgact gcgcgtcgat gtcggcaccg
    27061 tgcagcagca ggaaggtgac gatgtcgtcg tggccgccgg ttgccgcgta gatgagcggc
    27121 gtccagccgc gccgattcag gtcggcaccc cccgcctgca gcacccgcac ggtatcgaga
    27181 tgaccgcgca gggccgcgag catcagcggc gtatcgccgt atgcgttcgg cgtgttcgga
    27241 tgggccttga acgcgagcag cgtctgcgcc gtctgcgcgt agccgttgcg cgccgcgatg
    27301 cagagcatcg gctcgccgct cgcggcgacc gagtcggcgt ccaggccacg ggcgagaagc
    27361 ttcttcactt cgtcgacgcg gtcgttctcg accgcgagcg cgagctcgtc gagcagcggc
    27421 tcgcccgctg cggcgagcgg cagcgcaacg agcactgccg tcgccagcac gcggcgccgg
    27481 gttcggtcaa ccgtcatcgc gcccgatgcc gaagaggcgg aagaaattgt cggaggtggc
    27541 ctgtgcgatc gcttcgaccg gcacctcgcg cagcgccgcg atctcttcgg caacgtgacg
    27601 cacccacgcc ggctggttgc gcttgccgcg atgcggcacc ggggcgagat acgggctgtc
    27661 ggtctcgatc agcattcgcg acagcggcac ccgtttcgcc acgtccttca gcgcggttgc
    27721 attgcgaaag gtcacgatcc cggagaacga gatgtaaaag ccgagctcga tcgcgcgctg
    27781 cgcgacgtcc caggtttccg tgaagcagtg catcacgccg cctgcgtcgc ccgcgcgctc
    27841 ctcgcgcatg atcgcgatcg tgtcgtcggc ggccgcgcgg gtgtggatga ccagcggccg
    27901 gccggcctcg cgtgccgccc ggatatgggt gcgaaagcgc gcgcgctgcc attcgaggtc
    27961 accgctgcgg cggaaataat cgaggcccgt ctcgccgatg gcgacgacct tgcgctcgtg
    28021 cgcgcgcgca gccagcaggt cgacacccgg ttcatccgaa tcctcgtagt ccggatgcac
    28081 gccgacgctg gcatagaggt tcgcgtgctc gagcgcaagc gcgtgcactg cgggccatgc
    28141 gcccatctcc accccgatgc aaagcgcgtg cgtgacgcgg ctctcgcgca tcgcgtcgag
    28201 cacctgcggc aggtcgtcgc gcagttccgg gaaatcgagg tgacagtgcg aatcgacgaa
    28261 catgtcagga gccagaagtc ggaagtcaga gaacggcgcg cccgtggcgg cgctcggctc
    28321 ggcgcgcctt ggctgacttc tgtcctctga cttctgactg ctgatgccta catcgtatgc
    28381 gttgcgcgcg tcgaggcgag gtgctcgccg aggatgcgct cgatcgttgc cttcgccgcc
    28441 gcccccgtat cgtcgagcgt gaactgcacg ccgatgcctt gcgtgcggtt gccctgaccg
    28501 ccgtcgggcg tgatccagac gaccttgccc tgcacggcga tgcggttcgg gtcgtccatc
    28561 agcgacagca gcatgaatac ttcctcgccc agcgtgtacg gccgcgacgt gggaatgaag
    28621 atgccgccac ccttgaggaa cggcatgtac gcggcgtaga gcgccgcctt ttcgcgaatg
    28681 ttgagcgaca gcacgcccgg gcgggcaagt gccggtgtcg gtccaggaat cttgtcggcc
    28741 atggtcaccc aaacaatgcg cggtaatcga tcatgagcgc ctcggccacg agacgcggtg
    28801 acagcggatg cgcaagcagc gcccgctggc ggagcagttg ccgatgatat cgaaagagcg
    28861 cgggcggtgc caccgcacac gcgagctcgc gcagcgcggc ttcgaacgcc acgttctcca
    28921 acggctggcc cccggcatgc acgcacgcaa gatcggcgca ccacccgagc agccagtcga
    28981 tcacccctgc gagccggtcc ttccgctggt cgcgcgggcc ggcgtcgatt ctcgctccca
    29041 tcccggcgac ttcgagcgta gccggtgcag cgaacgcgcg tatccacgcg cttcgctcgc
    29101 cctgataggc gcgatcggcc agcgcgagcg cacgaagcgg cgcaccgtgc gcctgcgcga
    29161 gcgccgcgtc gggatcgtcc gtgccctgct gcaccagcca cgcacgggcc tgcgcacgcg
    29221 tcgggcgggg tgcgacgatg cgctggcagc ggctcgcgat cgtcgccggc agccgtccgg
    29281 gtaaatgcga aacgagcagc aggcaggtgc cgtcgggcgg ctcctcgagc gtcttcagca
    29341 gcgcgttggc cgcggcttca ttcatccgct cggccggcac gatgagcgcc accttcgcgc
    29401 caccgcggtg actcgtgatg gcggcccagc gcgtgagcgc gcgaatgcgg tccaccggga
    29461 tccattcgac gattttcgcg tcgccgtcgt cgtcgagctc gacgggctcg accgtccgca
    29521 gatcgggatg ctgccccgcc gccacgtagc ggcagcctgc gcactccgcg cacgccgcac
    29581 cgccggcgag cggcgattcg cagagcagcg ctccagcgag cgcttgcgcg agcatccgct
    29641 tgccgatgcc caccggaccg gtcacgagca gcgcatgcgg ccactgcgcg cgacgcgaca
    29701 gcagcgaggc gacgacgtcg cgctgccacg gcagtggtgc ccgcgcaggc tcggccggcc
    29761 ggccttcgcc gtcgcgcgtc atagcgtgtc gacgatgctg caaagctcgg cgcgcacttc
    29821 ggccaccggg cgggtcgagt cgatgatgcg aaagcgcgca ggctcggcag cggcgcgcgc
    29881 gagatagccg tctcgcaccc gcccgaagaa gctcgcggtt tcgcgctcga acttgtccgc
    29941 ggccttgccc ttcgcatgca tgcgcgccag ccgctcgcgc gagacctcgc tcggaacgtc
    30001 gaagagcagc gtcaactcgg gattgcactg gccatgcacc cacgcggcga gtgcgtcgat
    30061 catcgcgagc gacacgccgt gaccggcgcc ctggtatgca tacgtggcat cggtgaagcg
    30121 atcgcacagc acgatatcgc cgcgcgcgag cgcgggctcg atgacctgtt cgacgtgctc
    30181 ccggcgcgcc gcgaacatca gcaacgcctc ggcgtcgtgt gacagcgccc ggtggagcaa
    30241 ccactcgcgt atcgcttcac cggccgaggt gccgccgggc tcgcgcgtgg cgacgacgcg
    30301 tttgccgcgg gcgacgagcg tctcggcgag aaagcgcgcg tgcgtgcttt tgcccgcgcc
    30361 gtcgatgcct tcgagcgtga tgaagcggcc gcggggacgt tgatcgggca agagtctgtt
    30421 cggcgaaggg accggcctgc ggtgacgatc tgcggtcagc ggccgccgcg ctggtatttt
    30481 gacacggcgc gattgtgctc gatcaggttg gccgagaact gcgatgtgcc gtcgccgcgc
    30541 gaaacgaagt acagatacgg cgtcgccggc ggattgacga cgacgtcgag cgacgcctgc
    30601 gacgggagcg cgatcggcgt gggcggcagc cccgtgcgca cgtacgtgtt gtacggcgaa
    30661 tcggcttcga ggtcgcgctt gcgcagattg ccgtcgaagt gcgccccgat gccatagatg
    30721 accgcgggat ccgtctgcag gcgcatgccg cgcgcgagcc gattgacgaa caccgaggcg
    30781 atcagcggcc gatcctgcgc atggccggtc tccttttcca cgagggacgc caggatcagt
    30841 gcgtcgtagg gcgaagcgag cggcaggtcg ggcgcgcggt gcgaccatgc ggcgtcgagc
    30901 cttgcgtgca gcgcgcgata ggcgcgcttc agcaccgcga tgtcgctcgt gccggcggcg
    30961 aagtaatacg tgtcgggaaa gaacaggcct tcgggcgcag gctcggtcgc gccgaggcgg
    31021 gcgagcagtt ccgcatcgga gagcccgtcg gtctggtgcg cgacatccgg gttgtcgtcc
    31081 agtgcgcgct tcagctgtcc gaacgtcgtg ccctcgacga tcgtgatcgc ggtctgcgtc
    31141 acgtcgccct gcgtgagctt cgcgagcaac cgcgggagcg tgatcccctg ctcgatctcg
    31201 tagctgcccg ccttgatcgt gcggtcgacg tgcatcaccc gtgcaagcgc agtcagcgcg
    31261 atcgcatgcg gcacgacgcc cgccgcgtgc agcgatcgcg cgacgctcgc aagcgatgcg
    31321 ccgctgcgaa cctcgaagtc gaacggcgtg tgcggcagcg cgagcggctt gcggtacaac
    31381 gcgacgccga tcgccgccgc caccaccagc gaagcgacga gcagcaggac gacgacggtt
    31441 gcgacacggg acttgcgcat gccgatcgaa tggcgcgtgg atggcgcgaa gcgcccacgg
    31501 gcggcgcatc gcgcgtttga catggccgat tataatgggc cgatgattgc actcgcgccg
    31561 caccgctcgc tccgcgaccg cgtcggcctg caccgctgcc gcgcgcccgg ccgcgagcgc
    31621 gcacggggct tcacgctgat cgagatcatg gtggtgatcg tgatcctcgg cgtgctcgcc
    31681 gctctcgtcg tgccgagcgt cctgtcgcgc accgacgatg cccggatcgt cgcggcgaag
    31741 accgatctcg gcgcgatccg gcaggcactg aagctctacc ggctcgacaa tcagcgctac
    31801 cccacgaccg agcagggtct cacggcactg gcgacaaaac caaccgagcc gccggtgccg
    31861 ccgaactgga aaccgggcgg ctatctcgaa aaggttccgc tcgatccctg gggccgcagc
    31921 taccaatacc tgaatcccgg gctcaagagc gacgtcgacg tgttcacgta cggcgccgac
    31981 ggcgcacccg gcggcacggg tgccgacgcc gacatcggtt cgtgggacct ctaagacggt
    32041 tgggatgcct ttcccgaagc gattcgtcgg gcctggatcg tcgcgtgccg gaatgcatcc
    32101 gtgacgcccg tttcgccgac ctcgcgaacc ggcggcttca cgctcgtcga gatcctcgtc
    32161 gtcattgtca tcctcgggat cgtcgcgggg atttcgatcg cgctcgtcga acccggcgag
    32221 cgcgacgctt cggcgcgcga ggcgcgcagc ttcgccggcg cgcttgaata tgcggcggcg
    32281 cgcgcgcaat ggcgaaacga gatgctcggc gtatcggcgg acggtcgcgt ccttcgctac
    32341 tggcgccgcg atgccgcgaa cgaccgctgg gtcgtgctcg gcgacgacga cgtccttcgt
    32401 gcgcacgcgc tggctgcgcc ggtcgagggc atggcactcg cctacgccgg gcgcagcgtc
    32461 gcgtcgaacg ccatcgtgcc gttgcgcgca tccggacgca acgagccgtt cgccttcgcg
    32521 ctcgcgacgc cgcgctttcg cacggtcatc gcactcgatc cgttgaaccg cgcgtcgatc
    32581 gccggccccg acccgaacgg ctcgtgatgc gccgcgctcg cgcgttcacg ctgatcgaag
    32641 tgctggtcgc gctcgccatc cttgccgtcg cgctgagcgc cggcatgcgc gcggtggcgc
    32701 aaagcgcgga cggtgcaacg tcgctcaaga tgcgcacgct cgcgctttgg gtcgcgcaga
    32761 accggctcgc acaggcgcag cttgccgatc cgtggcccgc gcgcggggtc gccagcggcg
    32821 acgagacgca ggcgggcacc cgtctcgcgt ggcgcgagac ggtgagcgat acgcccaatc
    32881 ccgctttccg caggatcgag atcgtcgttt ccgagcctgg cgtgcccgac tacgcactcg
    32941 cccggctggt gggctacctc ggcaatgcgc atcgctgagc ggcgcgcgcg cggcttcacg
    33001 ctggtcgagg cgctcgtcgc tgtcgcgatc cttgcgatcg tcgcgacgat cgcgtggcgc
    33061 gcgacggcgg ccatgaccga cagcgaagcg cgccttgcga gcgaaagcgc gcgctggcgc
    33121 cggctcgacg cgctcatgac gcggatggaa gcggacatgc gcaaggcgat tccgcgcagc
    33181 gcccgccacg gtggcatcgt ggagccggca tggttggcgg gcccagccga cgccgcgggc
    33241 aacacggcgc tcgtcttcac gcgcgccggt cccgatgcga tcgacgagcc gggcagcggc
    33301 gggcagcgcg tcggttatcg gctgcgcgac ggccgcatcg agctcctgta ctggccacga
    33361 ctcgacaacg tcgcgacgcg cgacccggcc gcgtatgcgc tcgtcgacgg tgtcacgcgc
    33421 tttcacttgc tgcaactcgg cgcggacgag cgctggaccg accgctggcc gccttcgggc
    33481 gtcgacggcg tcccgcgtgg cgttcgcgtc gacatcgagc tcgccgacgg cagcgtcgtc
    33541 gagcgctggc tagcgctgca atgaaacccg cgtcgctttc gcggcccctc gcgtcgtccg
    33601 tgcgacagca gggtctcgcc ctgatcatcg cgatgctgat tgcagcactc gccgccgccg
    33661 ttgcggtatc cgtcgcaacg gcgcagtcgc agtggctcgc acaggtcgac catcggcgcg
    33721 atcaggtcga ggcgcagtcc atcgccctcg ccggcgttca gtgggctcgc gcgatcgtcg
    33781 acgccgatgc gcgcggcatc gatcaccttg gcgagccgtg ggcgctgccg ctgcccgcga
    33841 cacccgtcga gaacggcgag gtcgaaggcc gaatcgtcga tgcgcaggcg atgctcaacg
    33901 tgaacaatct cgcgggtgca cagcatggag cgctcgagcg tcggcgcttc acccgcctct
    33961 tcgccacgct cgggataccg gacacgacgc ttgcgtcgat cgtcgattgg gtcgacgcgg
    34021 acgacgtccc gcaacaaggt ggcgccgagg atgcgtggta cgcgggcgaa gcggatccgt
    34081 cgctcgcggc gaatgggccc gcgacccgga tcgaggaact ggcgctcgtg cgcacaatga
    34141 ccgcgcaagc ccttgcgcgc atcgtgccgt tcgtgacgag cctgccgccc gacacgccgg
    34201 tcaacgtcaa caccgcggcg cccgaggtgc tcgcggcgct gctggatggc atcgacggcg
    34261 gcgcacttgc ggcactcgtg gcgagccgtc acgaccatcc gttcgcgtcg caggaagact
    34321 ttcgggggcg gcttcccacc ggcgtcacgg tgatcgaccc ggacctgatc gcagtttcca
    34381 ccggctattt tttcgtgacc gttcgcgcgc ggcagggaga aacgatcgcg caggcacgtg
    34441 cgctgatcca ccgcgacaac gcgtcttcgg caatcgtctg gcaaacgatc gagtagcaag
    34501 cgtcacggcg ttcgtccgtt gcgccgcacg ttcccgtaaa ctcccggcga tgctgcgcgt
    34561 cttcctcccc gcaccgccgc acatcgatcg cgccgatcaa tgggtgcgtt acagcgccga
    34621 tggccgcgcg atcggccgcg gtcgggacgt gccgtcgcac tggccggccg atcctgtcat
    34681 cgaagccgtg ctcgcggcgt gccatgtaag gctcatagca cttgcgttgc cgccgatgcc
    34741 gcgcgagcgc ctgggtggcg cggcgcgcta cgcgctcgaa gatcagatcg cgacgagcat
    34801 cgacgaatcg tcgatcgccc tcgccgagcc gcgcaatggc acctgcgtcg ccgccgtcgc
    34861 atcggaggca ttgatccggg cagttgccac gcagacgcgt cgcatcgcac gcatcgttcc
    34921 ggaatcggcg ctcgcaccgc agggggacgg ctgggtatta tggtgcgcat cggccggcgc
    34981 cggcggcttc gtgcgccgcg cggacggcag cgcattcgcc gtggacgcat ccagcgacgg
    35041 cgcgctgccg ggcgcgctgg cggcggcact gcgcgcgaaa ggcggtccgc gaacggtgca
    35101 cgtggcgttt tccgccgatg cgccgcagct cgccgagtgg tcgcgcacga gcggtgtgcc
    35161 gttcgttggc gcaccggtct ggcattggga gcaggcaccc gccgccgcgt tcgccagcgc
    35221 tcccgacttc ctgcgcggaa cgacgccgcc tgcagacggt gcaagccgtc gctccatcct
    35281 gcgcagcttt cggcccgcgc tgattctcgc gggcgcagcg ctcgccattc atctcgccgg
    35341 cctgctcgtt acctggtcgt ggctcaacgt cgagaactgg cgcttgtcgc gcgcgatcgt
    35401 cgacgaagcc gcggcagcgc aattgcccga tgcgacgacg ccgcaggcgg ccttcgatgc
    35461 catcgcgcgt cgcaacgcgc agcggcgcca tcgcgcgctg caggccgcgc cgggcgatgc
    35521 gctacccctg ctcgcgcgcg ccgcgcccgc gctcggcgcg ttgccgcaac agacgctgcg
    35581 ctccgcgcga tatgcgagcg gcgcgtggac gctcgatctc ggcaaggtcg atcccgcgct
    35641 gttgtcgcgt gtcaccggtc ggctgacggc ggcgggcgtc gaggcgctgg cggtgccggc
    35701 ggcgggcgga acccggatgc ggctgtcgct cgccgctgtc gcgcgttgat gcccgtgcgc
    35761 tggccacgat cgatcacgca ggcggtgcag cgcgccgatt tttcccggcg ccccgctttt
    35821 gcgggactcg cgatcgtcgc ggcgctggtc gtggcagccg ttgttgcgct gcggctgcac
    35881 gacgcgatcg cgcgtgaacg cgagaacgtc acgcgcaacg gcctggtgct cggcgcagct
    35941 cgcgcacgcg ccgccgagaa cgcgacgctc gcccgaacta acgccgtcgc gaagaatgtg
    36001 gatctgcgcg cttcgatcga acaggttttc gcgcgccacg cgctggccta ccgcgttctc
    36061 gacgcgcagg aaagcgaagg gccgctgaga atcgtgatcg aggcggcgcc gttcgacgcg
    36121 ctggtgcgcg cgctcgacgc actctcgcgc gaggacggtg tgcgcgcgac cgacgcgacg
    36181 ctcaccgcgc gcgtcgaccc tggtacggtg cgcgccgagc tcgaactcgc gcgctgaagc
    36241 gacacgtgcg catcctgctg cgcctcgtgc cgggtctcgt cgtgcttgcc gtcgcactcg
    36301 tcgtgctcgc gcccgcggcc ctgctcgatg ctccgcttgc cgcacgcacc ggcaaccgct
    36361 tgcatcttgt cgacacccgc ggcgtctggt ggcgcggcga gggcgcgctc gcgacgagcg
    36421 acggcgccgc gcacgtgccg ttcgcgtgga aggttgcgct tgcgcccctt ttgaccggcc
    36481 gtctcgttgc cgcgttgcgc gcgtcggatg gcgcggccgg tccgacggga acgattgcat
    36541 tcgcggatgg gacgatcggc gtgcgcgatc tgcacgcggt cgtgcccgcc gcgctcgtcc
    36601 ccgcactcgc tccgtcactg aaggccgtgg cgctgcgcgg cgacatcgag ctctttgcgc
    36661 cgtcgttcac ctggcgcggc acgacgggaa gcggaacggt cgacgccacc tgggcgcatg
    36721 cacgcgtgat tgcaggcccg tgggccgtcg atttcggccg cgtcattgca aagggcgtac
    36781 cggccggcga cggcatcgcg ggcaccgtgc aggcttcggg cggcgatgtc acgatcgaag
    36841 gcacgctcga cacgcgcgcg ggcgccacga gcgcatcgct cgaactcgct ccggcgccgg
    36901 gcgcgtcgga
//
LOCUS       DASSVX010000202        12319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_941565, whole genome shotgun sequence.
ACCESSION   DASSVX010000202 DASSVX010000000
VERSION     DASSVX010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12319
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941565"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            484..1881
                     /gene="ltrA"
                     /locus_tag="VFO53_08700"
     CDS             484..1881
                     /gene="ltrA"
                     /locus_tag="VFO53_08700"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET8876391.1"
                     /translation="MKAEADPGDQASAADTGRYPGTAAARAEAELTTRARTKAEAAVS
                     GLMEAVVDRGNLWLAYQRVVANKGAPGVDALTVAEFKSYLQQHWPTIKGRLLAGTYRP
                     QPVRRVDIPKSQGGVRTLGIPTVVDRLIQQALHQVLQPIFEPTFSDASYGFRPGRTAH
                     QALRRAQQYVASGKRWVVDLDLEKFFDRVNHDLLMGKLAAKIDDARVLTLIRRYLEAG
                     MMADGLMQPRTEGTPQGGPLSPLLSNILLTALDRELERRGHAFCRYADDCNIYVASER
                     AGMRLMTRLTQFLEQRLKLKVNASKSAVARPWQRKFLGYSMTWHPQPKLCIAVPSLQK
                     LTEKVKTVLRGARGRSLHTTITLLNPLLRGWAAYFKLAATKQALETCDGWIRRKLRCL
                     LWRQWKRRAASRARVLMQRGLSAERAWRSARNGRGPWWNAGASHMNAAFPKSWFDHLG
                     LVSLLDTVQRFQRVL"
     gene            complement(1876..2460)
                     /locus_tag="VFO53_08705"
     CDS             complement(1876..2460)
                     /locus_tag="VFO53_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876392.1"
                     /translation="MHGAVPPPKRSSMSSGSACAIDHLPDSQQRVYHNQCNPRMCVRK
                     HSKRFIGKRLSNALDSGEVEYDRLETIDARKHPPGLRARHELVVAVPGKSDRDDCFGE
                     SVIIGLGARVPVDYFALRSDSIVLDPHVYGRSGVWRTQGVDPVFRLADLVAEIYGVRY
                     DPEFVYKERDRVGGGVTSAALPHHRTCGSAYGGS"
     gene            2483..2959
                     /locus_tag="VFO53_08710"
     CDS             2483..2959
                     /locus_tag="VFO53_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876393.1"
                     /translation="MNAYHPLWEIGIGTALVLLVVIVHGFAMFAIQNAFDRWFPQLVL
                     RPHRVLSKVLIGGIILSLLAVHYVEILMWAATIYGVKALPDMRSAFYYAGGAYTTYGS
                     GSLYLPENWRLLGFMIATSGLFTFAWTTGILISVMNRFLHARGGDAGTRLPDNRAR"
     gene            3108..4484
                     /locus_tag="VFO53_08715"
     CDS             3108..4484
                     /locus_tag="VFO53_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET8876394.1"
                     /translation="MTRQPNLLVILIDDLRFDEFEAGGHPYMKTPHIDRIANEGALFE
                     RAFHTTPICSPNRASILTGQYASRHGIIDNVARDAASHRLPNYHLALQELGYETAHIG
                     KWHMGNDGKPRPGYDHWVAYDGHGRLNDPKLNENGTYVQHHGYVTDIMNRMAVDFVTK
                     KHDKPWSLFFAHKAVHPDAEQAADGTFRMDGYRPAERHKDMYRGCVFPRKPNMLPPAD
                     VLKSKPAWAEAFALRKSDAARTMLDAIHAGEQEEIRLRAAMMAAVDEGVGMVFDALAS
                     TGQLDDTFILFLGDNGYFFGEHGLGPERRFAYEEGIRSPFIARFPRAIEAGTRIDRLV
                     LCQDIAPTLIELAGGRPGAQIQGASLLPLLQGGQRNWRKSFLVEYWAENAYPWLVGMT
                     YKAVRTDELKYVHWVNRGVCGELDEMYDLGRDPYELANVISHPAYADAREMLRAELRG
                     LVTSALGL"
     gene            complement(4588..5190)
                     /locus_tag="VFO53_08720"
     CDS             complement(4588..5190)
                     /locus_tag="VFO53_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876395.1"
                     /translation="MKRAGNVGNARQHLHLIRVIAGADDRTNGDGCERCTRRTSPFRH
                     SGGIAGRNASRADRASGIDPRVVGTRQFFRRRSRMDALVPRRLGRRGARVHALRQHDG
                     NPVGQHIRRCLTRRRGFNAAFDGAGRHSGSVSAARRRLRKFEIFSHCERRAALIAPID
                     GDVIHRLAFRTGLEVERRAALIAELRSCGIAVDTEVTACG"
     gene            complement(5187..5597)
                     /locus_tag="VFO53_08725"
     CDS             complement(5187..5597)
                     /locus_tag="VFO53_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876396.1"
                     /translation="MAAASLSIAGCAALSGVDGGSAPRGTFRRREVVDAGVLVAGGRV
                     LALGAPRRTDAGAEAEGSAEVDAALVVSPVAEAPGVAAEVAGAIAPGAAAADPGPRSP
                     RTVAAGAAGTVSGDDGEFAEDDAGAASDVAPLLK"
     gene            5789..6145
                     /locus_tag="VFO53_08730"
     CDS             5789..6145
                     /locus_tag="VFO53_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876397.1"
                     /translation="MADIVDFVALLAAMDMRSLQAIIATIDQMPGIMPALQAWIEHAA
                     RWEHDRRNGFHYPLQGPMAAIPPEEVPAALGASSMISHCFRHERRRDVTPVLAFFEGL
                     TRALAAEHERAGSAMH"
     gene            6142..6756
                     /locus_tag="VFO53_08735"
     CDS             6142..6756
                     /locus_tag="VFO53_08735"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET8876398.1"
                     /translation="MTPPRRCWWAPSGDALYADYHDREWGFPVADDRRLFEKLCLEGF
                     QSGLSWRTILARRDAFRTAFANFDFERVARFTASDIERLVADASIIRHRGKITSVRNN
                     ALRACDVASEHGSLAAYFWRFEPSKLHRPRRLARDTLAALPLPDEAIALSKDLKRRGF
                     TFVGPTTAYAFMQAMGLVNDHVDDCDVRQAAEKARRTFERPRAA"
     gene            complement(6761..7315)
                     /gene="efp"
                     /locus_tag="VFO53_08740"
     CDS             complement(6761..7315)
                     /gene="efp"
                     /locus_tag="VFO53_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET8876399.1"
                     /translation="MKIAQEVRAGNVIMIGTNPMVVQKAEFSKSGRNASVVKMKLRNL
                     LSGGGTETIYRADEKFDVIQLERKEVTYSYFADPMYVFMDQEFNQYDVEKENMEEALP
                     YLEDGLACELTFYEGKTISVELPTSVVREVTYTEPAVKGDTSGKVTKPAKLGSGFEVQ
                     VPLFVNTGDRIEIDTRTGEYRRRV"
     gene            complement(7385..8494)
                     /gene="earP"
                     /locus_tag="VFO53_08745"
     CDS             complement(7385..8494)
                     /gene="earP"
                     /locus_tag="VFO53_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HET8876400.1"
                     /translation="MNGGRWDIFCRVIDNYGDAGVCWRLARQLAAEHGLDVTLWIDRV
                     ATLSHLVPAIGDACDAQTAAGVVVRRLDDGATDASLPDMVVEGFGCGLPACYVDAMAA
                     AATPPPWINLEYLSAEPWVDTAHGLASPHPRLPLTRHFWFPGFTRCTGGLIREAGLLA
                     ARDAWRAHRARDADALRIVLFCYPNRALPALFDAWAEGDERIACIVPEGVATAALDAW
                     LRGDVPHAGQRALRGRLALEVVPFMTQDAFDRLLWGAGINFVRGEDSFVRAQWAARAF
                     VWQPYPQENDAHRLKLDAFVARYAHLLRAPARQALGAFWEAFNAEDGGAAARAWPAYR
                     AAVDAYEAHGRGWADTLASFPDLASGLVDFWRRRL"
     gene            complement(8504..9301)
                     /locus_tag="VFO53_08750"
     CDS             complement(8504..9301)
                     /locus_tag="VFO53_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876401.1"
                     /translation="MTQSTRDALLRVAAPFAVAVIVLAAWQVLVTVNRVPPYLVPSPL
                     VVAQTLVADRALLVHSLGVTLGIALTALALATLLGVATALLFVQSRWLEMSLFPYAIL
                     LQVTPIVSIAPLIIIWVRDTRVALVLCAVVVAIFPIISNTTLGLRSVDRGLSDLFRLA
                     RATRWQTLWRLRVPSALPYFFGGLRIASGLSLIGAVVAEFVAGTGGQGAGLAYQILLA
                     GIQLNIPRLFAALVLIALAGVALFAATVGLSRLALSRWHESALDGES"
     gene            complement(9298..10122)
                     /locus_tag="VFO53_08755"
     CDS             complement(9298..10122)
                     /locus_tag="VFO53_08755"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876402.1"
                     /translation="MTAPAIVALRGVAKTYDTGTQALDRVDLDIRRGEFLTLVGPSGC
                     GKSTLLHLVAGLARPSAGGITWWGGTFDATGGEGRRIGFVFQSPTLMPWASVAANVRL
                     PLDLAGASRAGAHAAVTRALARVGLGEASTHLPRQLSGGMQMRASIARALVTEPDLLL
                     MDEPFGALDEFTRQRLDSELLELWHAQRFTVVFVTHSIQEAVFLSTRVAVMAAHPGRI
                     LDTCDIGEPYPRDESFRLSPGFAVIAQQLSACVARASAGTLPDSAARRTKARPRLR"
     gene            complement(10151..11137)
                     /locus_tag="VFO53_08760"
     CDS             complement(10151..11137)
                     /locus_tag="VFO53_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876403.1"
                     /translation="MIRCLSKLAIAVVAVLALAVNAAHAQSLSKIVFSTDWLAQAEHG
                     GFYQAVAEGTYRKYGLDVDIRMGGPQVNGLQLLAAGRLDIAMADALQVMSAVEQRVPI
                     VAIAATFQKNPTVIIAHPGVARLEDLKGKPIAIGAASNTTFWPWLRGKYGFTDDQKRP
                     YGFSVQPFLADPALSQQGFVTSEPFTIEKGGVRPAVFLLADYGYPPYSEVLAVTRKTL
                     AGRRDALERFVRASAEGWKSYLANPAPGNALIKRANPQMSDELLAYGVQKMREYMIVT
                     GGDARTSGLLTMTAARWESTAGFLRAAGLSKPGVDYSRAYSLELVKAVHVLP"
     gene            11136..>12319
                     /locus_tag="VFO53_08765"
     CDS             11136..>12319
                     /locus_tag="VFO53_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8876404.1"
                     /translation="MLLFPEMTSRGWRARIVRAALCDSLNVPPTVSAWPRHARRPFGH
                     ALSRGNAAYFEGPLECTTMNAPVDMAIAECMQSLARILQLQRAAFDADRYPDFGTRRE
                     RLERLLQMLAHHEREWVGAIDRDFGHRATHETRLAEIGVVAGEARHALRNLARWMKPR
                     RVRMPPHLLPAAARILAQPLGVVGVIGAWNQPVQLALAPAVAALAAGNRVMVKPSERA
                     PATAALLAELVAAYFREDELTVVEGNASVASMFSALPFDHLCFTGSADVGRRVAAAAA
                     ENLTPVTLELGGKSPAIVDRSADLNEAAERIGYAKLLNAGQTCIAPDYAYVPEESVQA
                     FAEKVRTHMRRMFGTEPTNKDYTSVISDRHYARLEALVADAAAKGARILQWAKADDPN
                     WKA"
BASE COUNT         2198 a         4044 c         4093 g         1984 t
ORIGIN      
        1 cgcgcacgca atgacgcgca agcgctgttg cgcgcgaaga gcggccgatg cggccgctct
       61 ttttttggtg cgcccggcag ggcgcacgta cttggaggtg aaagtcctct gcacacccgg
      121 caaggggaag tgctagttga cggcaagggt gtcgcgggtg actgcgaatc cgaaggaagc
      181 cgaaggcaaa gcgctggcct gacgaacagg aagcggatag aggcggtgtc gcggggtaag
      241 gcggccaaaa aggtcaaagc ccaatacttg cacggaacgc gacgacgtag atccgacggg
      301 cataagcgtg aaggtgcgtg cgcaataccc ggggagatct gcacgtgtgc cttgtgctac
      361 cgacatcgag aggtgtgggg atgcatgtgc agaagtcagc cgaggccata gtaggcgtcc
      421 gtacgagccg aagggccgaa catgaaaggc cgcgagtagg cggcggaagt ctcagggaag
      481 tcgatgaagg cagaagctga tcctggtgat caggcgagtg cagcagatac cggacggtat
      541 ccggggacgg ctgctgctcg cgccgaggcg gaactgacga cccgcgcgcg gacgaaagcg
      601 gaagctgctg tatcgggcct gatggaagcc gtagtggatc gcggcaacct gtggcttgcg
      661 tatcaacggg tggtggcgaa caagggtgcg ccgggcgtgg atgcgctcac cgtggccgag
      721 ttcaaaagct acctgcagca acactggccc acgatcaagg gccgcctgct ggccgggacg
      781 tatcgaccgc agccggtgcg ccgggtggac atacccaagt cgcaaggcgg ggtgcgcaca
      841 ctcgggatac cgacggtggt ggatcggctc attcaacagg cgctgcatca ggtgctgcaa
      901 ccgatcttcg agccgacgtt ttcggacgca agctacggct ttcgaccggg gcgtacggcc
      961 catcaggcgc tgcgtcgcgc gcagcagtat gtggcgagcg gcaagcgctg ggtggtggac
     1021 ctcgatctgg agaagttctt cgatcgggtc aaccacgacc tgctgatggg caagctggcg
     1081 gcgaagatcg acgacgcacg cgtgttgaca ctgatccggc ggtatctcga agccggcatg
     1141 atggcggacg gtttgatgca gccgcgcacg gagggcacgc cgcaaggcgg accgctatcg
     1201 ccgctgctgt ccaacatcct gctgaccgca ctggaccggg agctggaacg acgcggccat
     1261 gcgttctgcc gctacgccga cgactgcaac atctacgtcg cgagcgagcg ggcagggatg
     1321 cgactcatga cacgtctgac gcagtttctg gaacagcggc tcaagctcaa ggtgaatgcg
     1381 agcaagagtg cggtggcgcg gccatggcag cggaagtttc tgggctatag catgacgtgg
     1441 cacccgcagc cgaagctgtg cattgctgtg ccgagcctgc agaagctgac ggagaaggtg
     1501 aagaccgttt tgcgaggcgc gcggggtcgc agtctgcaca ccaccatcac gcttctcaat
     1561 ccgctcctgc gtgggtgggc agcgtatttc aagcttgccg ccaccaagca agcgttggag
     1621 acgtgtgatg gctggatccg ccgcaaactg cgctgcctgc tatggcggca atggaagcgg
     1681 cgagctgcct cgcgagcacg cgttctgatg cagcggggct tgtcggcaga gcgggcgtgg
     1741 cgctcggctc gcaatgggcg tggcccatgg tggaacgcgg gtgcgagtca catgaacgcg
     1801 gccttcccca agtcctggtt cgatcatctg gggctggtgt cgctgctcga taccgtgcag
     1861 cgcttccagc gcgttttatg aaccgccgta tgcggaaccg cacgtacggt ggtgtgggag
     1921 ggcggcggag gtaactccgc cccctacccg atcccgctcc ttgtagacga attctggatc
     1981 gtatcggact ccgtatattt cagccacgag atcggccagc cggaagactg gatcgacgcc
     2041 ctgggtcctc cacacaccgg aacgtccgta aacatgcgga tcaagaacaa tactatccga
     2101 acgtaacgcg aaatagtcga caggcacgcg agccccgaga ccgatgatta cggattcacc
     2161 aaaacagtcg tccctgtcag acttgcccgg tacggcgacg accagctcgt gtctcgctcg
     2221 caagccaggc ggatgctttc gagcgtcgat cgtttcaaga cggtcatact cgacttcacc
     2281 ggagtcgagc gcattggaca ggcgtttgcc gatgaagcgt ttcgagtgtt tgcgaacgca
     2341 catccggggg ttgcattggt tgtgatacac gcgttgctgg ctgtccggca gatgatcgat
     2401 cgcgcaagcg ctgcctgatg acatggaaga ccgcttgggc ggcggcacgg cgccgtgcac
     2461 ggcgcactct ccgaatccag tcatgaatgc gtatcacccc ttgtgggaaa tcggcatcgg
     2521 cacggcgctc gtgctgctcg tcgtgatcgt gcatggcttt gcgatgtttg cgatccagaa
     2581 cgcgttcgac cggtggtttc cgcagctcgt gttgcgcccg catcgcgtcc tgagcaaggt
     2641 gttgatcggt ggcatcatcc tgagcctgct ggccgttcac tacgtcgaga tcctgatgtg
     2701 ggcggccacg atatacgggg tgaaggcatt gccggacatg cgaagcgcgt tctactacgc
     2761 ggggggcgcc tacaccacat atggctcggg gagcctgtac ctgcccgaaa actggcgcct
     2821 gctcgggttc atgatcgcga cgtccgggct cttcacattc gcgtggacga cgggcatcct
     2881 gatcagcgtg atgaaccgct ttcttcatgc ccgtggcggc gatgcaggca cgcgactgcc
     2941 cgacaaccga gcccgatgat gcagctgcac ggcccggccg agccgctcgg gcaacccgag
     3001 tcatccgtct tcgtgccggt gttcgccggt gcctacaatg tcgatctgct gcgcaagccc
     3061 gcatcagcgt agtcatccgc cttgcaaccc ttcgctatcc gccacgcatg acaagacaac
     3121 caaacctgct cgtgatcctg atcgacgacc tccgcttcga cgaattcgag gccggcggcc
     3181 atccgtacat gaagacgccg cacatcgacc gcatcgccaa cgaaggcgcg ctgttcgagc
     3241 gcgcgttcca tacgacgccg atctgctctc ccaatcgcgc atcgatcctc acgggacagt
     3301 acgcgagccg gcacggcatc atcgacaacg tcgcgcgcga cgcagcgagc caccgcctgc
     3361 ccaactacca cctcgcgctg caggagctcg gctacgagac ggcacacatc ggcaagtggc
     3421 acatgggcaa cgacggcaag ccgcggcccg gctacgacca ttgggtcgcc tacgacggtc
     3481 atggccgcct caacgatccg aagctcaacg agaacggcac ttacgtgcag catcacggct
     3541 atgtgaccga catcatgaat cgcatggcgg tggacttcgt cacgaagaag catgacaagc
     3601 cgtggtcgct gttcttcgcc cacaaggcgg tccatcccga tgcggagcag gcggccgacg
     3661 gcacgttcag gatggatggc taccgccctg cagagcggca caaggatatg tatcgcggtt
     3721 gcgtctttcc gcggaagccg aacatgcttc cgccggccga tgtgctgaag agcaagccgg
     3781 cctgggcgga agcgttcgca ctcaggaagt cggacgctgc acgcacgatg ctcgatgcga
     3841 tccatgcggg cgagcaggaa gagatccgcc ttcgtgccgc gatgatggct gcggtggacg
     3901 agggcgtcgg catggtgttc gacgcgctcg catcgaccgg gcagctcgac gacacgttca
     3961 tcctgttcct cggcgacaac ggctatttct tcggcgagca cgggctcggc cccgagcgcc
     4021 gcttcgccta cgaggaaggc atccgcagtc cgttcattgc acgcttcccg cgcgcgatcg
     4081 aggccggcac gaggatcgac cggctggtgc tgtgccagga catcgctccg acgttgatcg
     4141 agcttgccgg tggtcgaccg ggtgcgcaga tccagggcgc gtcgctgctg ccgctgctgc
     4201 agggcgggca acgcaactgg cgcaagtcgt tcctcgtcga gtactgggcc gagaacgctt
     4261 acccgtggct cgtcggcatg acctacaagg cggtgcgcac ggacgagctc aagtacgtgc
     4321 actgggtgaa ccgcggcgtg tgcggcgagc tcgacgagat gtacgacctc ggccgcgatc
     4381 cgtatgagct tgcgaacgtg atctcgcacc cagcgtacgc cgacgctcgc gaaatgctca
     4441 gggccgaact gcgcggactc gtcacctcgg cgctcggtct ctagcggcgc gcgggagaat
     4501 gtcgagcagc ccgcgcaaaa cctttcgagg aaccccggcc ccgacgctct ctctaatccc
     4561 tgccgtcgcg caacctacgt gcccatgtca tccgcaagct gtcacttctg tatccaccgc
     4621 aatcccgcag gatcgaagtt ctgcaatcaa tgcggctcgc cgctcgacct caagccctgt
     4681 gcgaaatgcg aggcgatgaa tcacgtcgcc gtcgatcggt gctatcaatg cggcgcgccg
     4741 ttcgcaatgg ctgaaaatct cgaatttgcg gaggcggcgg cgagcggcgg aaacgcttcc
     4801 gctgtggcgt ccggcgccgt cgaacgcggc attgaatccg cgtcgcctcg tcaggcatcg
     4861 gcgaatgtgt tgcccgaccg gattcccgtc gtgctgtcgc agcgcatgga cccgggctcc
     4921 ccgcctgccg aggcgacgcg gcacgagcgc gtccatccgc gagcggcggc gaaagaattg
     4981 ccgggtgccg acgactcgtg gatcgatccc cgaagctcga tcggcgcggc tcgcattacg
     5041 gcccgcaata ccgcccgaat ggcggaacgg cgaggtgcga cgcgtgcagc gttcgcaacc
     5101 gtcgccgttt gtgcgatcgt cggcgccggc tattacgcgt atcaggtgca aatgctgccg
     5161 cgcgttgccg acgttgcccg cgcgcttcat ttgagcaacg gcgcaacgtc ggatgcggcg
     5221 ccggcgtcgt cctcggcaaa ctcgccgtcg tcgccggata cggtacccgc ggcgcctgcg
     5281 gcgacagtgc gcggggagcg cgggccaggg tcggccgcag cggccccggg cgctatcgcg
     5341 ccggcaactt ctgctgcaac cccgggcgct tccgcaaccg gcgaaaccac caacgcagcg
     5401 tcgacttcgg cgctaccttc ggcttcggct cccgcatcgg tccgccttgg cgcgccgagc
     5461 gcaagcacgc ggccgccagc aacgagcacg ccggcgtcga cgacttcacg ccggcgaaac
     5521 gttccgcgcg gcgcgcttcc accgtcgacg cccgacagcg ctgcgcaacc ggcgatcgac
     5581 aaggacgcgg cggccacgca gcggttgatc gagcgcgacc tcgggcgctt ccttccgccc
     5641 gaacgcacgc agcaatctgc tccgcgctga gcctcgcgcg gccgtattcc cggaaagcag
     5701 ccgggcgcta ctgcgaggaa cgacaggggt gctcgcggcc gcaatctggc aaactcctgc
     5761 gaccttcctt acgtgccggg acaacttcat ggccgatatc gtggactttg tcgcattgct
     5821 cgcggcaatg gatatgcgat cgctgcaggc gatcattgcg acgatcgacc agatgccggg
     5881 catcatgccc gcgctgcagg cgtggatcga gcatgccgcg cgctgggaac atgaccggcg
     5941 caacggcttc cactatccgc tgcagggccc gatggccgca attccgcccg aggaggtgcc
     6001 ggctgccctc ggcgcgagct cgatgatcag tcattgcttc cgccacgagc ggcgccgcga
     6061 tgtcacgccg gtgctggcgt tcttcgaggg actcacgagg gcgctggccg ccgagcacga
     6121 gcgcgccggg tcggcgatgc attgaccccg ccgcgacgtt gctggtgggc gccgagcggt
     6181 gatgcgctct acgccgacta tcacgaccgg gagtggggct ttccggtcgc cgacgatcgg
     6241 cggctgttcg agaagctgtg tctcgaaggc ttccagtcgg gcttgagctg gcgcacgatc
     6301 ctcgccaggc gcgacgcatt tcgcacggcg ttcgcgaatt tcgatttcga gcgcgttgca
     6361 cgcttcacgg cgagcgacat cgagcgcctc gtcgccgatg caagcatcat ccgccatcgc
     6421 ggcaagatca cgtcggtgcg caacaacgcg ctgcgtgcct gcgacgtagc gagcgagcac
     6481 ggctcgctcg ccgcctattt ctggcgcttc gagccgtcga agctgcatcg cccccggcgg
     6541 cttgcgcgcg acacgctcgc cgcgttgccg cttccggacg aggcgatcgc gttatcgaag
     6601 gacctgaagc ggcgcggctt cacgttcgtc ggaccgacga cggcttacgc gttcatgcag
     6661 gcgatgggcc tcgtcaacga ccatgtcgac gactgcgacg tccggcaggc ggccgagaag
     6721 gcgcgccgca ccttcgagcg cccgcgcgcg gcgtaacgcg tcagacgcgg cgtcgatact
     6781 cgccggtacg cgtgtcgatc tcgatcctgt cgccggtgtt gacgaagagc ggcacctgca
     6841 cctcgaaccc gctgccgagc ttcgcgggct tcgtgacctt gcccgacgtg tcgcccttga
     6901 ccgccggctc ggtatacgtg acctcgcgta ccaccgacgt gggcagctcg accgagatcg
     6961 tcttgccctc gtagaacgtg agctcgcacg cgaggccgtc ctcgagatag gggagcgcct
     7021 cctccatgtt ttccttctcg acgtcgtact ggttgaactc ctggtccatg aacacgtaca
     7081 tcgggtcggc gaagtacgag tacgtgacct ccttgcgctc gagctggatc acgtcgaact
     7141 tctcgtcggc acgatagatc gtttcggttc cgccacccga cagcaggttg cgaagcttca
     7201 tcttgacgac cgacgcattg cggcccgatt tgctgaattc ggccttctgc acgaccatcg
     7261 gattggtgcc gatcatgatg acgttgccgg cgcggacttc ctgggcgatt ttcatgggat
     7321 tcctgaaggc ggttgccgtc gagctttggc ggcggccgca tagcgatttt ggggaaaccg
     7381 gtaattataa tcgacggcgc cagaaatcga ccaacccgct ggcgaggtcg gggaatgacg
     7441 cgagcgtgtc cgcccagccg cgcccgtgcg cctcgtaggc atcgactgcg gcgcgatagg
     7501 cgggccacgc ccgcgccgcc gcgccgccgt cctcggcgtt gaacgcttcc cagaatgcgc
     7561 cgagcgcctg ccgggccggc gcgcgcaaca ggtgcgcata gcgcgcaacg aaggcgtcga
     7621 gtttcaatcg atgcgcgtcg ttctcctgcg gatacggctg ccacacgaac gcgcgcgccg
     7681 cccattgcgc acgcacgaac gaatcctcgc cgcggacgaa gttgatgccg gccccccaaa
     7741 gcagccggtc gaaggcatcc tgagtcatga acggcacgac ttcgagggcg agccggccgc
     7801 ggagcgcgcg ctggcccgca tgcggcacgt caccgcgcag ccacgcatcg agcgccgcgg
     7861 tcgccacacc ttcgggcacg atgcaggcga tgcgctcgtc accttcggcc cacgcatcga
     7921 agagcgccgg caatgcgcgg ttcggatagc agaagagcac gatgcgcaag gcgtccgcat
     7981 cacgcgcccg atgcgcgcgc cacgcgtcgc gggcagcgag caggcccgct tcgcgaatga
     8041 ggccgccagt gcagcgcgtg aagccgggaa accagaaatg acgcgtcagc gggagccgcg
     8101 ggtgcggcga tgcgagaccg tgcgcggtat ccacccaggg ctcggccgac aggtattcga
     8161 ggttgatcca tggcggcggc gttgccgccg ctgccattgc atcgacgtag cacgcgggca
     8221 gcccgcagcc gaagccctcg acgaccatgt ccggcagcga agcgtccgtt gcaccgtcat
     8281 ccagtcgacg cacgacgacg cccgccgcgg tctgcgcgtc gcaggcgtcc ccgattgcgg
     8341 ggacgaggtg cgaaagtgtg gcgacacgat cgatccacag cgtgacatcg aggccatgct
     8401 cggcggcgag ttgacgcgca agccgccagc acacgcccgc gtcgccgtag ttgtcgatga
     8461 cccggcagaa gatgtcccac cggccgccgt tcatcccggc agtttacgac tccccgtcga
     8521 gcgcgctttc gtgccagcgc gacagcgcga gccgcgacaa gcctacggtg gccgcgaaca
     8581 gcgcgacgcc ggcgagcgcg atcagcacca gcgccgcgaa cagccgcgga atgttgagct
     8641 ggatgcccgc gagcaggatc tggtaggcga ggcccgcgcc ctggccgccg gtgccggcaa
     8701 cgaattcggc gacgaccgcg ccgatcagcg acagcccgct cgcaatgcgc aggcccccga
     8761 aaaaatacgg cagcgcactc ggcacgcgca gtcgccacag cgtctgccag cgcgtggcgc
     8821 gtgcaaggcg gaaaaggtcg gaaagtccac ggtcgacgct gcgcaagccg agcgtcgtgt
     8881 tcgagatgat cgggaagatc gcgacgacga ccgcgcacag cacgagcgca acccgcgtgt
     8941 cgcgcaccca gatgatgatc agtggggcga tcgacacgat cggcgtcacc tgcagcagga
     9001 tcgcgtatgg aaacaggctc atctcgagcc agcggctttg cacgaacaag agcgcggtcg
     9061 ccacgccgag aagcgtcgcg agcgccaacg ccgtcagcgc gatgccgagc gtgacaccga
     9121 gcgagtgcac gagcagcgcg cgatcggcaa caagcgtttg ggcgacgacg agcggcgagg
     9181 ggacgagata cggcggaacg cgattgaccg tgacgagcac ctgccacgcg gcaagcacga
     9241 ttacggccac cgcgaacggc gccgcgacac gaagcagcgc gtcacgcgtg ctttgcgtca
     9301 tcgcagtcgc gggcgcgcct tcgtgcgcct cgccgcgctg tcgggcagcg tgccggcgct
     9361 cgccctggca acgcatgcgg acagttgctg cgcgatcacg gcaaacccgg gtgacaggcg
     9421 aaacgactcg tcgcgcggat acggctcgcc gatgtcgcac gtgtccagaa tgcgccccgg
     9481 atgcgccgcc atcacggcga cgcgcgtcga caggaacacg gcttcctgga tgctatgggt
     9541 cacgaacacg acggtgaagc gctgcgcgtg ccacagctcg agcagctcgc tgtcgaggcg
     9601 ctgtcgcgtg aattcgtcga gcgcgccgaa cggctcgtcc atcagcagca ggtcgggctc
     9661 ggtcacgagc gcccgggcaa tcgacgcgcg catctgcatg ccgcccgaaa gctgacgtgg
     9721 aagatgcgta ctcgcctctc cgaggccaac gcgcgccagc gcgcgcgtca ccgcggcgtg
     9781 cgcgccggcg cgcgaggcgc cggcgagatc gagcggcagg cgcacgtttg cggcgaccga
     9841 cgcccacggc atcagcgtcg gcgactgaaa cacgaacccg attcgccggc cctcgccgcc
     9901 ggtcgcgtcg aacgtgccgc cccaccacgt gatgccaccc gccgatggac gtgcaagccc
     9961 ggcgacaaga tgcagcagcg tcgacttgcc gcagcccgag gggccgacca gcgtcaggaa
    10021 ctcgccgcgc cggatgtcga gatcgacgcg gtcgagggcc tgcgtgccgg tatcgtacgt
    10081 cttcgcgacg ccgcgcagcg cgacgattgc cggcgccgtc acatcatcgc ttgcgccggc
    10141 ccgcgccatc ctaaggaagg acgtgcacgg ccttgacgag ctcgagcgag tacgcgcggg
    10201 aataatcgac gcccggtttg gacagtcccg ccgcgcgcag gaagccggcc gtcgattccc
    10261 agcgcgcggc cgtcatcgtc agcagtcccg atgtgcgcgc atcgcccccg gtgacgatca
    10321 tgtattcgcg catcttctgc acaccgtagg cgaggagctc atcgctcatc tgcggattcg
    10381 cgcgcttgat cagcgcattg cccggggcgg gattggcgag gtagctcttc catccttccg
    10441 cggacgcgcg aacgaatcgc tcgagcgcgt cgcgccgccc ggcaagcgtc ttgcgcgtga
    10501 cggcgagcac ctccgaatag ggcgggtagc cgtagtcggc gagcaggaac accgccggcc
    10561 gcacgccgcc cttttcgatc gtgaatggct cggaggtgac gaagccctgc tgcgacaacg
    10621 caggatcggc aagaaacggc tgtaccgaaa agccgtacgg acgtttctga tcgtcggtga
    10681 atccgtattt cccacgcagc catggccaga acgtcgtgtt gctcgccgcg ccgatggcga
    10741 tcggcttgcc cttgagatcc tcgaggcgtg caacgccggg atgggcgatg attaccgtcg
    10801 gattcttctg gaacgtcgcg gcgatcgcca cgatcggaac gcgctgctcg acggccgaca
    10861 tcacctgcag cgcgtcggcc atcgcgatgt cgagccggcc ggcggcgagc agctgcaggc
    10921 cgttgacctg cgggccgccc atgcggatat cgacgtcgag gccgtatttg cggtaggtgc
    10981 cttccgcgac cgcctggtag aagccgccgt gctccgcctg agccagccag tcggtcgaaa
    11041 acacgatctt cgaaagcgac tgcgcgtgcg ccgcattcac ggcgagcgcg agcaccgcca
    11101 cgacggcgat cgcaagcttg ctgaggcatc gaatcatgtt gttgttcccg gaaatgacgt
    11161 cgcgaggatg gcgggcgcgc atcgtgcgag cggcattatg cgacagcctg aatgttcccc
    11221 caaccgtgtc ggcgtggccc cgacacgcac gtcgtccctt tggtcacgca ctttcgcgag
    11281 gcaatgctgc ctattttgaa ggccccctgg aatgcacaac gatgaacgcg ccggtcgaca
    11341 tggcgatcgc cgaatgcatg caatcgctcg cgcgcatcct gcaactgcag cgcgcggcgt
    11401 tcgacgccga ccgctatcct gatttcggca cgcgccgcga gcggcttgaa cggctgctcc
    11461 agatgctggc gcaccacgag cgcgaatggg tcggcgcaat cgatcgcgac ttcggtcacc
    11521 gcgcaacaca cgagacgcgg ttagccgaga tcggtgtcgt ggcgggcgag gcgcgacacg
    11581 cgctacgcaa tctcgcgcgc tggatgaagc cgcggcgcgt gcgcatgccg ccgcacctgc
    11641 tgcccgccgc ggcgcgcatc ctggcgcaac cgctcggcgt cgtcggcgtg atcggcgcct
    11701 ggaatcagcc ggtgcaactc gcgctcgcgc ccgccgttgc ggcgctcgcg gcgggcaacc
    11761 gcgtcatggt taaaccgtca gaacgcgcgc cggcgacagc ggcgctgctc gccgagctcg
    11821 ttgccgccta tttccgcgaa gacgagctca ccgtcgtcga aggcaatgcc tccgtggcca
    11881 gcatgttttc ggcgctgccg ttcgatcacc tgtgcttcac cggctcggcc gacgtggggc
    11941 gccgcgttgc cgcagcggcc gcggagaatc tcaccccggt gacgctcgag ctcggcggca
    12001 agtcgccggc gatcgtcgac cgctccgctg acttgaacga ggccgccgag cgcatcggct
    12061 acgccaagct gctcaatgcc gggcagacct gcatcgcgcc ggactatgcc tatgtgccgg
    12121 aagaatccgt gcaggcgttc gccgagaagg tgcgcacgca tatgcgccgc atgttcggca
    12181 ccgagccgac aaacaaggac tacacctcgg ttatctccga tcgtcattat gcgcggctgg
    12241 aagcgctggt ggcggacgcc gcggccaagg gcgccaggat cctgcaatgg gccaaggcgg
    12301 acgatccgaa ctggaaggc
//
LOCUS       DASSVX010000203         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_475067, whole genome shotgun sequence.
ACCESSION   DASSVX010000203 DASSVX010000000
VERSION     DASSVX010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475067"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..786
                     /locus_tag="VFO53_08770"
     CDS             166..786
                     /locus_tag="VFO53_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876405.1"
                     /translation="MLALERYAAPKIALCAAVGACLLTTAVFAVTSREAARRVDAYAA
                     QHLHDWTIVDRVLTPLPMNPFCWELILIESNQRDYALRRAIVSLAPSRMASLQCPMRT
                     MMGAISAPLRRVAAPDTAVVHWHGEIVLSRDALRDLARTSCEAAALLRFARAPWYAEN
                     GDRRVIGDLRYDREPALGFAEIDLAHAARCPDYVPPWVPPRSDLLR"
     gene            complement(798..1691)
                     /locus_tag="VFO53_08775"
     CDS             complement(798..1691)
                     /locus_tag="VFO53_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET8876406.1"
                     /translation="MTTGEIGASIRARIPYEAITRRPALRLPNDARVAVWTIVNVESW
                     NPGAPMPRAVLPPPMGQPLLPDVPNWAWHEYGMRVGFWRFLETLSSRGLKATFAVNGS
                     ACAVYREACVAACDAGWEFMGHGFVQKPMHKVDDQREAIGATMRSIAELTGKPPRGWE
                     SPGLTETDDTIDLLAEAGIEYVADWVLDEQPVPIGTRSGAIVSIPYTVEINDVVMSAV
                     QQQPSDEILRRGRDQFDRLYLDGAKSARVMAISIHPYLTGVPHRIRYLEALYDYILGH
                     EGVVMWTGAEILDWYRSQRVT"
     gene            complement(1678..2730)
                     /locus_tag="VFO53_08780"
     CDS             complement(1678..2730)
                     /locus_tag="VFO53_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8876407.1"
                     /translation="MRIDVHSHVIPARMIDAIARSPGEFAARVEGDAHARRIVHEQGY
                     VYPVFAEFHDTEAKLESMDRKGIDISVISPAPPMFYYWADVGLAAKVARLVNDGLADM
                     VGAKPSRLRGMATIPLQHADAAIAEMERVVRDYGFKAIEIGTSVEGLQLAEPRFRPVL
                     RRAQELGVFLFAHPYYVGAKSGLECYYLTNLIGNPLDTTTMLANLMFSGALDELDELK
                     LCLAHGGGFTPYQIGRLVHGHRVRAEARARSLSPPDALLRRLYFDSLVFDPLALRYLL
                     DLVGADHVCIGTDSPFDMGDETPVETINAVPRLTAGEREDLCCRTALHLLGEHVSDPV
                     AAGARHAPKGGTHDDR"
BASE COUNT          456 a          958 c          960 g          508 t
ORIGIN      
        1 gaaccgctgt actgggccgc ggccgcaccg ctcgtcttcc tgttcacgag ctacccggca
       61 cgcgcattcg tcgtgctcgc cttggtcgcg ggactctatc ttgccttcgt caccggtatg
      121 gtcgcggccg gctcgatcgc attctagtgc ctgcttgtcg tggcgatgct cgcgctggaa
      181 cgctacgcgg cgccgaagat cgcactctgc gccgccgtcg gcgcgtgcct tctgacgacg
      241 gcggtgttcg ccgtgacgag ccgggaagcg gcgcgccgcg tcgacgcgta tgcggcgcag
      301 catctgcacg attggacgat cgtggatcgc gtgctcacgc cgctgccgat gaaccccttc
      361 tgctgggaac ttatcctgat cgaaagcaac cagcgcgatt acgcgttgcg ccgcgcgatc
      421 gtgtcgctcg cgccgtcacg gatggcgtcg ctgcaatgcc cgatgcgtac catgatgggc
      481 gcgattagcg cgccgctgcg gcgcgtcgcc gcgccggaca cggccgttgt ccactggcac
      541 ggggagatcg tcctgtcgcg cgatgcgctg cgcgatctcg cccggacctc gtgcgaagcc
      601 gcggcgctgc tgcgctttgc acgcgcgccg tggtacgccg agaacggcga tcggcgcgtg
      661 atcggcgatc tgcgctacga tcgggagcct gcgctcgggt ttgcggagat cgacctcgcg
      721 catgcggcgc gctgcccgga ctacgtgccg ccgtgggtgc cgccgcgaag cgatctattg
      781 cgctgatgcc atggcggtta cgtcacgcgc tgcgaccgat accaatcgag gatttcggca
      841 cccgtccaca taaccacgcc ctcgtggccg aggatgtaat cgtagagcgc ttcgagatag
      901 cggatgcgat gcgggacccc ggtgagatac ggatggatcg agatcgccat gacgcgcgcc
      961 gattttgcac cgtccaggta cagccggtcg aactgatcgc ggcctcgacg caggatctcg
     1021 tcggacggct gctgctgtac ggcgctcatc acgacgtcgt tgatctcgac cgtgtaggga
     1081 atcgagacga ttgcgcctga gcgcgtgccg atcggcaccg gctgttcgtc gagcacccag
     1141 tcagcgacgt attcgatgcc cgcctccgca agcaggtcga tcgtgtcgtc ggtctcggtc
     1201 aatcccggac tttcccagcc gcgcggcggc ttgcccgtca attcggcgat cgagcgcatc
     1261 gtggcgccga tcgcttcgcg ctggtcgtcg accttgtgca tcggcttctg cacgaagccg
     1321 tgccccatga attcccagcc cgcgtcgcat gccgcaacgc atgcttcacg atagaccgcg
     1381 cacgcgctgc cattcaccgc aaacgtcgcc ttcaacccgc ggctcgagag cgtctcgagg
     1441 aagcgccaga agccgacgcg catgccgtat tcgtgccacg cccagttcgg cacgtccgga
     1501 agaagcggct ggcccatggg cggcggaaga accgcccgcg gcatcggtgc gcctggattc
     1561 cagctttcca cgttgacgat cgtccacact gccacgcgcg cgtcattcgg caggcgcaat
     1621 gcgggccgcc gcgtgatggc ctcgtacgga atgcgtgcgc ggatgctcgc gccgatttca
     1681 ccggtcgtca tgggttcctc ctttcggggc gtgtcgtgca cctgcggcca cgggatctga
     1741 cacgtgctct ccgagcaagt gcaacgcggt gcggcagcaa agatcctcgc gttcgcccgc
     1801 ggtgagtcga ggcacggcgt tgatcgtttc gaccggcgtt tcgtcgccca tgtcgaacgg
     1861 cgagtcggtg ccgatgcaaa catggtcggc gcccacgaga tcgagcaggt agcgcagtgc
     1921 gaggggatcg aacacgaggc tgtcgaagta caggcggcgc agcaatgcat cgggcggcga
     1981 cagcgatcgc gcacgagcct ccgcccggac ccgatgtccg tggaccagac ggccgatctg
     2041 atacggcgtg aagccgccgc cgtgcgcgag gcacagcttc aattcgtcga gctcgtcgag
     2101 cgccccgctg aacatgaggt tggcgagcat ggtcgtcgtg tcgagcgggt tgccgatcag
     2161 gttcgtcagg tagtagcact cgagtccgct cttcgccccg acgtagtagg ggtgcgcaaa
     2221 caggaagacg ccgagctcct gcgcgcggcg cagcacgggg cggaatcgcg gctccgccag
     2281 ttgcaagcct tccaccgacg tcccgatctc gatcgccttg aagccgtagt cgcgaacgac
     2341 gcgctccatc tcggcgatcg ccgcgtcggc gtgctgaagg ggaatcgtcg ccatgccgcg
     2401 cagtcggctc ggcttcgcgc ccaccatgtc ggcaaggccg tcgttgacga ggcgcgcgac
     2461 ctttgccgcg agacccacgt ccgcccagta gtagaacatc ggcggcgcgg gcgagatcac
     2521 cgagatgtcg attcccttgc ggtccatcga ctcgagtttc gcttccgtgt cgtggaactc
     2581 ggcaaacacc ggatagacgt acccctgctc gtggacgatg cgccgcgcgt gcgcgtcgcc
     2641 ctcgacgcgc gcagcgaact cgccggggga gcgcgcgatc gcgtcgatca tccgtgcagg
     2701 aatcacgtga ctgtggacgt cgattctcat gccgtggcga gtgcaaccgg atcgatgatc
     2761 agcgccggca gccgacgagg tcgacttcga cgcggcagcc gggcacgagc agtccgctca
     2821 cctgcagcgt cgtgcgcggc ggcggggacg ggaagaattc cttccacacc tggtcgaagt
     2881 ag
//
LOCUS       DASSVX010000204         5222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1158290, whole genome shotgun sequence.
ACCESSION   DASSVX010000204 DASSVX010000000
VERSION     DASSVX010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158290"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFO53_08785"
     CDS             complement(<1..483)
                     /locus_tag="VFO53_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8876408.1"
                     /translation="MKALIVDDHPLIRDAVANVLRSLDPAVDISVAGDCDSGFEIAAR
                     GAELDLLLLDLNLPGLSGIPALKRWRSQYPSVPVVVLSASDDQPTVLAAISGGAAGYV
                     PKSSSNEVMREALRLVLAGGKYLPPELLMPPPAATRSSKSQRQAIEHAGSLGLTERQI
                     A"
     gene            802..1284
                     /locus_tag="VFO53_08790"
     CDS             802..1284
                     /locus_tag="VFO53_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876409.1"
                     /translation="MKSVTFRRSIMAATGVALMLGTSLAAAQAAQPTLEGTWNVTRHG
                     VDCVTGEVRSSFPAIMVFHEGGTYSGFGASPGSTPADGSPEFGSWKRVPGAQSYKFRI
                     TGIAYDGGAPAGSVDINGAAQLSPQGERLDYTATIAFLDLNGDTLFTLCGRATGVRFQ
                     "
     gene            1414..3636
                     /locus_tag="VFO53_08795"
     CDS             1414..3636
                     /locus_tag="VFO53_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8876410.1"
                     /translation="MPTLTRHSLFWKYAAYFAGVVSALLVISGAVGGYFAYRESVTAL
                     EDVQQAKAQFAAADIESFMRGVQDALHAIVGKFNANGPVALEDLQLELVALLRHHPEV
                     SGVRWIDAGGDERIALSRFDRNVVDAARNWSAEPGFRTARKTSSYVGPIHFRKETEPY
                     VSVAAARDPGGSVLVAEVDLKYVWDVVSQVQLTHDGVAYVVDRAGELISHPDIGLVLR
                     KTDLSTLPQVRRALDRTSQGTLVIAEARDLAGVPVVSTAAPIDSLGWVVFAEEPLDEA
                     LRPVYASLERSVALVVLGLIAAIAASILLARRMVRPIREIETRARQIGEGRFDRRIAL
                     STGDELEALAHQFNLMAGRVQDTYAMQETRIAERTRELALANEAKTRFLAAASHDLRQ
                     PMHALALFVGQLRGVELPPDATTLLEKIERSVDALNELLEALLDLSKLDVGAISAKRG
                     PLALNDLLSRLASEFAPSAEAKGLALTLAPTSLWADSDPVLLQRILLNVIANALRYTR
                     DGRILIGCRRRGDEAEVIVADTGIGIDAVHLPQVFEEFYTAVPTQAGGNSGLGLGLAI
                     VKRLAALLDHRVSIESTLGKGTLVRIRVPRAKPVEAARPLELPIAEPLRGVRVLIVDD
                     ESAARDAMQGLLARWGCDVAAAESGDAAIEHAREQPPDVVLCDLRLRDGETGIDVVDR
                     LKRECRSAAYALVTGESSPEYLAQARTTGDPIAFKPTTPGKLRALLEHLVGAKQAITG
                     "
     gene            complement(3626..4600)
                     /locus_tag="VFO53_08800"
     CDS             complement(3626..4600)
                     /locus_tag="VFO53_08800"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876411.1"
                     /translation="MKRRSFLGIVCVAPGAPVRAAVSRATIHRVGYLHPTDAHDVAYV
                     AFRTALERAGYVIGANTILEERFAEGDMPRLPALAAELVAKRVDVIVAVSPTAIRAAR
                     AATRTIPIVMAFSGDDPVTSGFAATLARPGGNTTGLTTVALDMAPKWIELLADLRPGL
                     EAIAVLRAPRRPDHDAQIAVMRAAAQSQGIRLDAAEVSSVEQYARAFDGLVDAGCEGI
                     VVLAGPEFTHNRVRLVELVNAHRLPSVYQFSDFVLVGGLASYGPGIADLSARAVAYVD
                     KILNGANPAELPIEQPRRLFLVLNRKTANALGIAIPPRILLQADEIIQ"
     gene            complement(4872..>5222)
                     /locus_tag="VFO53_08805"
     CDS             complement(4872..>5222)
                     /locus_tag="VFO53_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET8876412.1"
                     /translation="DRTIALLKPGVTTDTIAKSLPIAGDIGFASEMDAFGLNFCHGLG
                     LGLHERPLISRLNSLKEPIELKAGMVFAVETYCPASDGVSAARIEEEVILTPGGPQVI
                     SLYPAQELPIANPY"
BASE COUNT          865 a         1767 c         1759 g          831 t
ORIGIN      
        1 ggcgatctgg cgctcggtga gcccgagcga cccggcatgc tcgatcgcct gccgttgcga
       61 tttcgacgat cgcgttgcgg ctggcggcgg catgagcaac tccggcggaa ggtacttccc
      121 gccggcgagc acgagacgca gcgcttcgcg catgacctcg ttggaagacg acttgggaac
      181 gtagccggcg gccccgccgc tgattgcggc gagcacggtc ggctgatcgt cggaggccga
      241 caaaaccacg acgggcaccg acgggtattg cgatcgccaa cgcttcaacg ccgggatgcc
      301 cgacagtccg ggcagattca aatcgagcag cagcagatcg agctccgctc cgcgcgctgc
      361 gatctcgaat ccgctgtcgc aatcaccggc aaccgaaatg tcgacggcgg ggtcgagact
      421 gcgcagcacg ttcgccaccg cgtcccggat cagcggatgg tcgtcgacga tgagcgcttt
      481 cacgtcagac gggaagggcc gccccaggca actggctcgc cccttcgggg gcagcggacg
      541 gagtgagcgt ggggagatgt tgcatccggc actttcgggc tggcgccatt gggccacgaa
      601 atccgagcca aagcgagcat ttcaccgtcg attcaagccg gtagccaatc cagcgccgcc
      661 gtgtcgcgcc gattccccgc gcgggttcgt actgcaccgg cccatcggtg caattcacgg
      721 catagcgacg cgcccgtaac gtcgctcgca cggtcgtgca gtccgcacgt ccggccgagt
      781 gatcttccag ggaggcttct catgaaatcg gtgacgtttc gcaggtcgat tatggcagca
      841 acgggtgtgg cgctgatgct cggtacgtcc ctcgcggcgg cgcaggcggc gcaaccgacg
      901 ctcgaaggca catggaacgt aacgaggcac ggcgtcgatt gcgtcaccgg cgaggtgcgc
      961 agctcgttcc cggccatcat ggtgttccac gagggcggaa cctacagcgg cttcggggcc
     1021 tcgccgggct cgacgcccgc cgacggaagt ccggaattcg gaagctggaa gcgcgtgccg
     1081 ggtgcgcaga gctacaagtt ccggattacc ggcatcgcgt acgatggcgg cgccccggca
     1141 ggcagcgtcg acatcaacgg cgccgcacag ctgtcgccac agggtgaaag gctcgactac
     1201 accgcgacca ttgccttcct cgatctgaac ggcgacacgc tcttcacgct gtgcggaagg
     1261 gcgacgggcg tccggttcca gtaggactcg cgatccgcgg cgccgaggac gccccggcgc
     1321 gtcctcgcgc cgcgcgttcg ccgtcgggta tgctccgcga tcgattcgac ggtttcgatc
     1381 cgatcaccga tacgcatcgc cggaatagtc ccaatgccga ccctcacccg gcactcgctg
     1441 ttctggaagt acgccgccta tttcgcaggc gtcgtcagcg cgctcctcgt catcagcggt
     1501 gccgtcggcg gctatttcgc gtatcgcgag tcggtcacgg cgctcgagga cgtgcagcaa
     1561 gccaaggcgc agttcgccgc ggccgacatc gagagcttca tgcgcggcgt gcaggacgcg
     1621 ctgcacgcga tcgtcggcaa gttcaacgcg aacggtccgg tcgcgctcga ggatctgcag
     1681 ctcgagctcg tcgcgctgct gcgccaccat cccgaggtca gcggagtccg ctggatcgac
     1741 gccggcggcg acgagcggat cgcgctgtcc cgattcgacc gcaacgtggt cgacgccgcg
     1801 cgcaactggt ccgccgagcc aggatttcgc accgcgcgca agacgtcgag ctacgtcggc
     1861 ccgatccatt tccgcaagga gacggagccg tacgtgtccg tcgccgcagc gcgcgatccg
     1921 ggcggcagcg tgctcgtcgc cgaggtcgat ctcaagtacg tgtgggacgt cgtgtcccag
     1981 gtgcagctca cgcatgacgg cgtcgcctac gtcgtcgatc gcgccggaga gctgatctcg
     2041 caccccgaca tcgggctcgt gctgcggaag accgatctgt cgacgctgcc gcaggttcgt
     2101 cgcgcgctcg atcggacgtc gcaggggacg ctcgtcatcg ccgaagctcg ggacctggcc
     2161 ggcgtgcccg tcgtgtccac ggcagcgccg atcgacagcc tcggctgggt cgtgtttgcc
     2221 gaggagccgc tcgacgaagc gttgcgcccc gtgtacgcat cgctcgaacg ctccgtcgcg
     2281 ctcgtcgtgc tcggcctgat tgccgccatc gcggcgtcga tcctgctcgc gcgacggatg
     2341 gtgcggccaa tccgcgaaat cgaaacgcgt gcgaggcaga tcggcgaagg ccggttcgac
     2401 cgccgcatcg cgctttcgac cggcgacgag ctcgaagcgc tcgcgcacca gttcaatctc
     2461 atggcgggac gggtgcagga cacgtacgcg atgcaggaaa cccgcatcgc ggagcgcacg
     2521 cgcgagcttg cgctcgccaa cgaagcgaag acgcgctttc tcgccgctgc cagccacgat
     2581 ttgcgccagc cgatgcacgc gcttgcgctg ttcgtcggac agcttcgcgg cgtcgagctt
     2641 ccgcccgatg cgacaacgct gctcgaaaag atcgagcggt cggtcgatgc gctgaacgag
     2701 ctcctcgagg cgctgctcga cctttcgaaa ctcgacgtcg gcgcgatcag cgcgaagcga
     2761 ggaccgctcg cgttgaacga tcttctgtcc cgtctcgctt ccgagttcgc accgagcgcc
     2821 gaggcgaaag gcctcgccct cacgttggcg cccacgtcgc tatgggccga ctccgacccc
     2881 gtgttgctgc aacgcattct gctcaacgtc atcgccaacg cgctgcgcta tacgcgcgac
     2941 ggccgcatcc tgatcgggtg ccgccgccga ggcgacgagg ccgaagtgat cgtcgcggac
     3001 accggaatcg gcatcgatgc ggtgcacctg ccgcaggtat tcgaggagtt ctacaccgca
     3061 gtgccgacgc aggccggtgg caacagcggg ctcggactcg ggcttgccat cgtcaagcgg
     3121 ctcgctgctt tgctggatca ccgcgtcagc atcgaatcca cgctcggcaa aggcacgctg
     3181 gtgcgcattc gcgtcccccg cgcgaagccg gtggaagcgg cacgcccgct cgaactgccg
     3241 atcgccgagc cgctgcgcgg cgttcgcgtg ctgatcgtcg acgacgaatc ggccgcgcgc
     3301 gatgcgatgc agggattgct tgcccgatgg ggatgcgacg tcgcggcggc cgagagcggc
     3361 gatgcagcca tcgagcacgc tcgcgagcag ccgcccgatg tcgttctttg cgacttgcgg
     3421 ctgcgcgacg gcgagacggg catcgacgtc gtcgacaggc tgaagcgcga gtgccgctcg
     3481 gcagcgtatg cgttagtcac cggtgaatcg tcgcccgaat atctcgcgca ggcgcggacg
     3541 accggggatc cgatcgcgtt caagccgacg acgccgggca agctccgcgc actgctcgag
     3601 cacctcgtgg gcgcaaagca ggcgatcact ggatgatctc gtcggcctgc agcaggatcc
     3661 gcggcgggat cgcgataccg agcgcattgg cggtcttgcg gttcagcacg agaaagagcc
     3721 ttcgcggctg ctcgatcgga agctcggccg ggttcgcgcc gttcaggatc ttgtccacgt
     3781 aagcgacggc gcgcgccgac agatcggcga tgcccgggcc gtacgacgcg agtccgccga
     3841 caagcacgaa atccgagaac tggtacaccg agggcagccg gtgcgcgttc acgagttcga
     3901 ccaggcgcac gcgattgtgc gtgaactcgg ggccggcgag cacgacgatt ccctcgcatc
     3961 ccgcgtcgac gagaccgtcg aacgcgcgcg cgtactgctc gacgctgctc acttcggcag
     4021 cgtccaggcg aatgccctgg ctttgtgcgg ctgcgcgcat gaccgcgatc tgcgcgtcat
     4081 ggtccggccg gcgcggtgcc cgcaggacgg cgatcgcctc gaggccgggt cgaagatccg
     4141 ccagcaactc gatccatttc ggcgccatgt cgagcgccac cgtggtcaat ccggtggtgt
     4201 tgccgccggg acgggcgagc gtggcggcga agccgctcgt gaccggatcg tcgccgctga
     4261 acgccatcac gatcggaatc gtgcgcgttg ccgcacgtgc cgcgcgtatc gcggtcggcg
     4321 aaacggcaac gatgacgtcg acacgcttcg ccaccagctc cgccgcgagc gcgggcaagc
     4381 gcggcatgtc gccctcggcg aagcgctcct cgagaatcgt gttggcgccg atcacgtaac
     4441 cggcgcgctc gagcgccgtc cggaatgcca cgtaggccac gtcatgcgcg tcggtggggt
     4501 gcaggtagcc gacacgatga atcgttgctc gcgaaacggc agcacgtaca ggcgcgcctg
     4561 gcgcgacgca gacgatgccg aggaaggaac gccgcttcat gtcacagtca ttcgctttcg
     4621 ccaacacgta cgcgcggata gcggccacct gtgctcgcgg gccgaggccg cgaactatgt
     4681 caccgccggt ccgcgatgtc gaccgggctt acacccgtcg ctcgcgccca gcaatccggt
     4741 cgataccgac gtcgcgtacg gcggcagtcc ttccgcctac ggcaccgcgc acgatccgat
     4801 ggtcggacgg cgcatcggcg gcgtcaacgt gttcggcggc ggcctcggcc tgtacaacgc
     4861 gaatcagaac gtcagtacgg attggcgatc ggcagctcct gcgccggata aagcgagatg
     4921 acctgcggcc cgccgggcgt gagaatcacc tcttcctcga tgcgcgccgc cgacacgccg
     4981 tcgctggccg ggcagtaggt ctcgacggcg aacaccatgc ccgccttcag ctcgatcggc
     5041 tccttcagcg agttgagccg cgagatcagc ggccgctcgt gcaggccgag gccgaggccg
     5101 tggcagaaat tgaggccgaa cgcgtccatt tcgctcgcga agccgatgtc gcccgcgatg
     5161 ggcagcgact tcgcgatcgt gtccgtcgtg acacccggct tcagcaacgc gatcgtgcgg
     5221 tc
//
LOCUS       DASSVX010000205         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1308743, whole genome shotgun sequence.
ACCESSION   DASSVX010000205 DASSVX010000000
VERSION     DASSVX010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308743"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="VFO53_08810"
     CDS             <1..1377
                     /locus_tag="VFO53_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET8876413.1"
                     /translation="GDVLITNNPWKGSGHLNDVQLVKPVFHGNRLVAFAACCAHVPDI
                     GGRIRSVEPREVFEEGFHIPPMKLLREGVPDESLLTLMRTNVRTPDQTVGDLFAQVGA
                     LEVMEQRLRTLMDDYGLHSLDEFADELYRRSDAAMREAVRALPDGTYRYEMVTDGLDE
                     PFTFRIALTIEGDGIDVDYAGTSPQQSRGINVVYAYTFAMTAYALKCALLPDLPNNEG
                     MFRALTVRAPEGSLLNARFPASVGGRICSGDYLPSLVFGALHQVIPDRVSAAPGSPLW
                     SMVISGVREDGKPYANVLFYNGGMGATSRKDGVSCYSWPNNIASTPVEVTERDTPFFV
                     RYKRLRENSGGEGRHCGGLGQDILLESRSPTPMAAVFLAERTRMPAPGLAGGGAGGLG
                     DVQINGRSIDVRRLHLLNEGDELLVRTPGAGGYGDVRERSAESIAHDRLEGWRHPLPE
                     DGVDGDAR"
     gene            1405..>2149
                     /locus_tag="VFO53_08815"
     CDS             1405..>2149
                     /locus_tag="VFO53_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876414.1"
                     /translation="MDDSPTMEANGQQSMRNRVLLAIAAHRTPGYHFPGHLLDARWPE
                     VGPEHARLVLPLAPHLTDSDGRLDLVALAVFADMALGTAARVRDAHHERQATLELQLQ
                     LTGARATSDVSALVRPRDGCTQSDGYSLTEATFEAAGKPIAYALGKFARLRAPEDVVL
                     APLPWQREPVASQVALDEQQLDPDEREALERCAAALPREGASESLVCRLWGGERVADT
                     SEPARRLTIGMHATNRVGHVQGGVLVALAV"
BASE COUNT          345 a          699 c          743 g          362 t
ORIGIN      
        1 ggcgacgttc tgatcacgaa caatccgtgg aagggttcgg gacacctgaa cgacgtgcag
       61 ctcgtcaagc cggtgttcca cgggaatcgg cttgtcgcgt tcgcggcgtg ctgcgcgcac
      121 gttcccgaca tcggtggccg catccgctcg gtcgagccgc gcgaggtgtt cgaggaaggc
      181 ttccacatcc cgccgatgaa gctgctgcgc gagggcgtac ccgatgagtc gctgctgacg
      241 ctgatgcgca cgaacgtgcg cacgcccgac cagacagtcg gcgacctctt cgcgcaggtg
      301 ggcgcgctcg aggtgatgga gcagcgcttg cgcacgctga tggacgatta cgggctgcat
      361 tcgctggatg agttcgccga tgagctgtat cgccgatccg acgcggcgat gcgcgaggcc
      421 gtgcgcgcgc tgccggacgg cacgtaccgc tacgagatgg tcaccgacgg tctcgacgag
      481 ccgttcacgt tccgaatcgc gctgacgatc gagggcgacg gaatcgacgt cgactatgcc
      541 ggcacgtcgc cgcagcagtc gcgcggcatc aatgtcgtct atgcgtacac gttcgcgatg
      601 accgcgtacg cgctcaagtg cgcactgctg cccgatcttc caaacaacga gggcatgttc
      661 cgggcgttga ccgtacgtgc acccgagggt tcgctgctta atgcacgctt cccggcatcg
      721 gtcggcggac gcatctgcag cggcgattac ctgccgtcgc tcgtgttcgg cgcgctgcat
      781 caagttattc ccgatcgcgt ttccgccgcg cccggatcgc cgttgtggag catggtaatc
      841 agcggcgtgc gagaggatgg caagccgtat gcgaatgtgc tcttctacaa cggcgggatg
      901 ggcgcgacgt cgcgcaagga cggcgtcagc tgttactcgt ggcccaacaa catcgcgtcg
      961 acgccggtcg aggtcaccga acgcgatacc ccgttcttcg tccgctacaa gcggctgcgc
     1021 gagaactcgg gcggcgaggg acgccattgc ggcggcctgg gccaggacat cctgctcgaa
     1081 agccgttcgc cgacaccgat ggcggccgta ttccttgccg agcgtacgcg aatgcctgcg
     1141 cccggcctcg ccggcggcgg tgccggcggc cttggcgacg tgcagatcaa cggccgctcg
     1201 atcgacgtgc gccgtctgca tcttttgaac gagggcgacg agctgctcgt ccgcacgccg
     1261 ggagccggcg gttacggcga cgtgcgcgag cgcagcgccg aatcgatcgc gcacgaccgg
     1321 ctcgaaggct ggcggcatcc gcttccggag gatggcgtgg acggcgatgc acgctaggct
     1381 ctgcccagcc aggcggccat cgtgatggac gattcgccga cgatggaggc caacgggcag
     1441 caatcgatgc gcaatcgggt gttgctcgcg atcgccgcgc atcgcacgcc gggttaccac
     1501 ttccccggtc acctgctcga tgcgcggtgg cccgaggtcg gtcccgagca cgcgcgctta
     1561 gtgcttccgc ttgcgccgca cctgaccgat agcgacggcc gcctcgacct cgtcgcgctt
     1621 gccgtgttcg ccgacatggc gctcggaacc gcagcgcgcg tgcgcgatgc acatcacgag
     1681 cggcaggcga cgctcgagtt gcagctgcag ctgaccggcg ctagggcaac cagtgacgtg
     1741 tcggcactgg tgcggccgcg cgatggatgc acccagtccg atggctattc gctgaccgag
     1801 gcgacattcg aagccgccgg caaaccgatc gcctatgcgc tcggaaagtt tgcgcgattg
     1861 cgcgcgcccg aggacgtagt tctcgcgccg ttgccctggc agcgcgagcc cgtcgcttcg
     1921 caggtggctc tcgacgagca gcaactggat cccgacgagc gcgaagcatt ggaacgatgc
     1981 gctgcggcgc tcccgcggga gggcgcatcg gaatcactgg tgtgccgcct gtggggcggc
     2041 gagcgcgttg ccgacacttc ggagccggcg cgtcgcctca cgatcggaat gcacgctacg
     2101 aatcgggtgg gacacgtgca aggcggagtc ctggtggcgc tcgcagtga
//
LOCUS       DASSVX010000206         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_58557, whole genome shotgun sequence.
ACCESSION   DASSVX010000206 DASSVX010000000
VERSION     DASSVX010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58557"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFO53_08820"
     CDS             <1..279
                     /locus_tag="VFO53_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET8876415.1"
                     /translation="ATRSLEEAAKNPEAAVDAMLKAKPKSGVRDTALVGMKETARLYK
                     GPDNPNDRPLRVGAKNMSETLALLTQYGGVDKSEAGKPEDYYTNAYLP"
     gene            303..1130
                     /locus_tag="VFO53_08825"
     CDS             303..1130
                     /locus_tag="VFO53_08825"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876416.1"
                     /translation="MRLADESTAQTPDAQAAARRPLIEVDRVAKTYVTADGPVESLRP
                     LTFAIDDGEFLAIVGPSGCGKSTLLKMVAGLLPASSGDIRVGGTRVDGPPDNVGIVFQ
                     SAVLLAWRTVLDNVMLQIEMRHLDKTKYLPKAKALLHLVGLDGFERKLPWQLSGGMQQ
                     RASICRALVHDPAVLLMDEPFGALDAMTREKMNVELQRIWFETKKTVLFITHSIPEAV
                     FLADRVLVMSERPGTIAAIYDVNLTRPRSLRVMSQPQFGELTQTIRRHFYAQGTLDA"
     gene            1159..>2613
                     /locus_tag="VFO53_08830"
     CDS             1159..>2613
                     /locus_tag="VFO53_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004675879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase"
                     /protein_id="HET8876417.1"
                     /translation="MSAHAPVFRIAEIDCFERPVRYRLPFRFGAATVAEGVQAFVRAR
                     IVGEDGRSAEGASAELMVPKWFDKRPALSNDDNIDELRMSLALARAAYLSDRAPLTAF
                     GHSAAHIAHCQVEGNLRGLPPLAAAFGAAEIDKAILDACCNALGISFFAAVQSNAIGF
                     APAGVAADVNDADADAFVRTLHPRPSIAVRHTVGLADVLRGHPATVNDSLPESLEEVI
                     ERHGVHWFKIKLAGDPPADAARVGDIARVLDRLPRYGVTLDGNEQYESVDALRELFDR
                     LKAMRALGRLCASLAFVEQPLPRRRTFQEDVDARAPAALLIDEADATLGAFPRARACG
                     YRGASSKSCKGLYKSLVNALRCAVWNRTGDGRYFLSAEDLTTPAGLALQQDLALASVL
                     GLEHIERNGHHYIDGMASAPEHEQTSFLDAHPGLYERSHGAVRLAIRDGTLALNSLSQ
                     PGFATGATPDWDTLAPMQAPVSTRSFAAPPGEPAA"
BASE COUNT          423 a          902 c          874 g          414 t
ORIGIN      
        1 gcgacgcgct cgctcgagga agccgcgaag aatcccgagg cggcggtcga cgcgatgctg
       61 aaggcgaagc ccaaatcggg cgtgcgcgac accgcgctcg tcggcatgaa ggagacggcg
      121 cgactttaca agggtcccga caatccgaac gaccggccgc tgcgcgtcgg ggcgaagaac
      181 atgtccgaaa cgctcgccct tctgacgcag tacggcggcg tcgacaaatc ggaggccgga
      241 aagcccgagg attactacac gaacgcgtac ctgccctgat cggcgcacgg acgatcgcgc
      301 cgatgcggct tgccgacgaa tccactgcgc agacgcctga cgcacaggcc gccgcccggc
      361 gtccgctgat cgaagtcgat cgcgtcgcga agacctacgt caccgccgac ggtccggtgg
      421 agtcgctgcg gccgctcacg ttcgcgatcg acgacggtga attcctcgcc atcgtcggcc
      481 cgtcgggctg cggcaagtcg acgctgctca agatggtggc ggggctgctg cccgcatcat
      541 cgggtgacat ccgcgtcggc ggcacgcgcg tcgacggtcc gccggacaac gtcggcatcg
      601 tcttccagag cgccgtgctc ctcgcatggc gcaccgtgct cgacaacgtg atgctgcaga
      661 tcgaaatgcg ccacctcgac aagacgaaat acctgccgaa ggcgaaggcg ctgctgcatc
      721 tggtcggcct cgacggattc gagcgcaagc ttccgtggca gctttcgggc ggcatgcagc
      781 aacgcgcgtc gatctgccgc gcgctcgtgc acgatcctgc cgtgctgctg atggacgagc
      841 cgttcggcgc gctcgacgcg atgacgcgcg agaagatgaa cgtcgagctg cagcggatct
      901 ggttcgaaac gaagaaaacg gtgctgttca tcacgcacag cattcccgaa gccgtgtttc
      961 ttgccgatcg cgtgctcgtc atgtccgagc ggccgggcac catcgccgcc atctacgacg
     1021 tgaacctgac gcggccgcgg tcgctgcggg tgatgagcca gccgcagttc ggcgagctca
     1081 cgcagacgat ccgccgccac ttctacgcgc agggcacgct cgacgcctga cgcccagcac
     1141 tgcaccatcg cgcgctgcat gtccgcccac gctcccgtct tccgcatcgc cgagatcgac
     1201 tgcttcgagc ggccggtgcg ctaccggctg ccctttcgct tcggcgcggc aaccgtggcg
     1261 gaaggcgtgc aggcgttcgt gcgcgcacgg attgtcggcg aagacggccg ttcggcggaa
     1321 ggcgcgagcg cggagctgat ggtgccgaag tggttcgaca agcggccggc gctgtccaac
     1381 gacgacaaca tcgacgagct gcgcatgtcg ctcgcgctcg cccgtgcggc gtatctgtcc
     1441 gatcgtgcgc ccctgaccgc attcggacat tcggccgcgc acatcgcgca ctgtcaggtc
     1501 gagggtaatt tgcgcggtct gcccccgctc gccgcggcgt tcggcgcagc ggaaatcgac
     1561 aaggcgatac tcgacgcgtg ctgcaacgcg ctcggcattt ctttcttcgc cgccgtgcaa
     1621 agcaacgcga tcggctttgc gccggccggc gtcgcggccg acgtgaacga cgccgacgcc
     1681 gatgcgttcg tgcgcacgct gcatccgcgc ccatcgatcg ccgtgcggca taccgtcggc
     1741 ctcgccgacg tgctgcgcgg tcatcctgcg acggtcaacg acagcctgcc cgaatcgctg
     1801 gaagaggtca tcgagcgcca tggcgtgcac tggttcaaga tcaagctcgc gggtgacccg
     1861 ccggccgatg cggcgcgcgt cggcgacatc gcgcgcgtgc tcgaccgcct gccgcgctac
     1921 ggtgtcacgc tcgacggcaa cgagcagtac gaaagcgtcg atgcgctgcg cgagctcttc
     1981 gaccggctga aggcgatgcg cgcccttggt cgcctctgcg catcgctcgc cttcgtcgag
     2041 cagccgctgc cacggcggcg cacgttccag gaggacgtcg atgcacgcgc gccggcggcg
     2101 ctgttgatcg acgaggccga tgcgacgctc ggtgcgtttc cgcgtgcccg cgcatgcggc
     2161 taccgcggcg cgtcgagcaa gagctgcaag ggtctctaca agtcgctcgt caatgcgttg
     2221 cgctgtgcgg tgtggaaccg caccggcgat ggacgttact tcctgtcggc ggaggatctc
     2281 accacacccg ccgggctcgc gctccagcag gatcttgcac ttgccagcgt gctcggcctc
     2341 gagcacatcg agcgcaacgg ccatcactac atcgacggca tggcgagcgc gcccgagcat
     2401 gagcagacgt cgtttctcga cgcgcatccc ggcctgtacg agcgctcgca cggtgcggtg
     2461 cggctcgcga ttcgagacgg cacgctcgcg ctgaactcgc tgtcgcagcc gggtttcgca
     2521 accggcgcga cgcccgattg ggacacgctg gcgccaatgc aggcgccggt atcgacccgg
     2581 tcgttcgcgg cgccaccggg tgagccggcg gcc
//
LOCUS       DASSVX010000207        19236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_25351, whole genome shotgun sequence.
ACCESSION   DASSVX010000207 DASSVX010000000
VERSION     DASSVX010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 19236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19236
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25351"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="VFO53_08835"
     CDS             <1..1310
                     /locus_tag="VFO53_08835"
                     /inference="COORDINATES: protein motif:HMM:NF022783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HET8876418.1"
                     /translation="NKLAQGEARLGPGRYFDLKAIKQPIIVFASMGDNITPPQQAFNW
                     ISDLYSSTEEIKANGQTIVGLIHEDVGHLGIFVSGKVAKKEHTQIVSVLKYIQALPPG
                     LYGMEIAERIGADGNVEYDVTLHERRLEDLKRLQKYDRVDEKPFEAVAALSELNERAY
                     ELLVRPLVREAMPEWLGKVLREWHPLRVQRWWFSDRNPLFAGLPAMAAAARAHRRPRS
                     ADNLGRRTEQLASGYVTAALDLYRDLRDAASEAAFYEMYGNLLSLSMADQRAMIRRQT
                     PFDARALPAVRQVLDEIDQGGLPEAIIRTGILIVKAGGGKRRLAQMEHTRALLQPTGV
                     LRDIDEDHFRRLLHEETLVVEFEPKLAKASLPKLMHNASDRQKLNAIFDWLETEAGLD
                     ARQQTLLGDLRKLVPRPAAALPRARQTPKGKASGRRAAIATAE"
     gene            1320..2732
                     /gene="fumC"
                     /locus_tag="VFO53_08840"
     CDS             1320..2732
                     /gene="fumC"
                     /locus_tag="VFO53_08840"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET8876419.1"
                     /translation="MANASESGVRIEKDALGEVRVPAEHLWGAQTQRSIENFPIGVER
                     FRWGRPVIRAFGVLKKAAALANGELGELAPDKVALIARAAQEVVNGTLDGEFPLVVFQ
                     TGSGTQSNMNANEVIANRAIQLAGGAVGSKKPIHPNDDVNRGQSSNDTFPAVMHVATV
                     FELDRRLLPALERLRTTLDAKAHAFAEVVMIGRTHLQDATPVTLGQIVSGWVAQLDDA
                     RTLIEFTRSGLMPLALGGTAVGTGLNAHPRFGATVAAVIARETGVPFVSAPNKFAALS
                     AHDAIINVSAALRTLAAALMKIANDVRWHASGPRAGLGEITIPDNEPGSSIMPGKINP
                     TQCEAVTMVAMRVYGNDTAVAFAGSQGNFQLNVYKPVMLQGTLESVELLADAIDSFDQ
                     RCAQGIEPAMERIRANLDRSLMLVTALNPHIGYENAAKIALAAHREHSTLREAALKLG
                     LVSAEDFDRWVDPAAMTRPG"
     gene            2732..3982
                     /locus_tag="VFO53_08845"
     CDS             2732..3982
                     /locus_tag="VFO53_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8876420.1"
                     /translation="MRIVALTFGTEGDTRPLAALCRGLLDAGHDVLLLGERSGHAYAD
                     ALHVPFVALAGDMTAELRAAPGTLHRGDEFRHVARALAAIATRHTALWMRETLARAKN
                     ADAIVCAGLAIYVGLSCAECLGIPAIGAGLQPMMPTRAFASPFLPPRRLPGFVNWVSH
                     RLVLAMMWRAFRKAINDARRDVAHQPSRRREWNGYPILFAISPTLVPRPRDWPERVSV
                     TGYWWARSDPHFVPDAALEAFLAAGEAPVYVGFGSMAGFDRDRLLADVLGALDGRRAL
                     LHAGWSGFGEGRLPGHVHRIGHVPHDWLFPRVGIVVHHGGSGTTHAAARAGVPSVVVP
                     FAADQFFWADRLHRVGIAPAGLAYRDVTPLRLRERLAKASGNGMRERANEIGRCVAAE
                     RGVANAVARIDALLSPRRAAQQAA"
     gene            complement(3986..5161)
                     /locus_tag="VFO53_08850"
     CDS             complement(3986..5161)
                     /locus_tag="VFO53_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HET8876421.1"
                     /translation="MELIPEITALDAEMQAWRHHLHAHPETAFEEEATSAFVAEKLRA
                     FGLEVYTGLAKTGVVGVLRAGRAQAAIGLRADLDALPIDERSGVAHASQNPGRMHACG
                     HDGHTTMLLGAAKALAARRNFDGTLHFIFQPAEENEGGGRVMVEEGLFDRFPMRAVYG
                     MHNWPRLPAGTFAMRAGPLMGAYDIFEIVATGKGAHAAMAYQGKDPMLFAAHAIHALQ
                     TIVSRNLHPQDAGVVSVTQVHAGDTWNVIPETIVLRGTVRTFEPGVQDLIERRMRTLV
                     DGIAAMFEMRASLRYERRYPATVNSEDETARARDAAAAVVGIDNVDTNPTPEMGSEDF
                     AFMLQQKPGCYVWLGAGRGPDTPNIHNPHYDFNDAVLPIGASYWVTLAERELPKKRA"
     gene            5261..6754
                     /gene="glpD"
                     /locus_tag="VFO53_08855"
     CDS             5261..6754
                     /gene="glpD"
                     /locus_tag="VFO53_08855"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HET8876422.1"
                     /translation="METRPFDLLVIGGGINGAGIARDAAGRGLSVVLVEKDDLAAHTS
                     SASTKLIHGGLRYLEHYEFRLVAEALAEREVLLRAAPHLIEPLPFVLPHEPHLRPSWM
                     IRAGLLMYDRLGGRMTLPKSFSVRLDGTRYGAGLKPRFRKGFVYADARVDDARLVVCN
                     ALDAHAHGADIRVRTKLAAARRDNGLWHATLVDAAGASSGITARALVNAAGPWVRAVL
                     GELTDVPVKAGVRHVKGSHIVVPRIHDAPHAYILQNADNRIVFVIPYQDDYSLIGTTD
                     VPVEHYAHPVISDEETYYLLTLANTYLERPLAPSDIVWSYSGVRPLYDDGTSDPSAIT
                     RDYVLQLDTARDAANAPVLSIFGGKITTYRKLAEAALAELRPFFPAMGSAWTHAAPLP
                     GGDIPDGDRDGWLAELRRRYPALPADLLRALARRHGTRALAVLADARSPADLGEDFGS
                     QLTEREIRYLQRDEWAATAEDVLWRRTKCGLAMTREACARVGEYLGQ"
     gene            6763..7563
                     /locus_tag="VFO53_08860"
     CDS             6763..7563
                     /locus_tag="VFO53_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HET8876423.1"
                     /translation="MPPRPLAQMPLAARRAIRFVLTDIDDTLTTDGKLTVEAYAALAR
                     LSASGRIVVPVTGRPAGWCDHIARMWPVDAVVGENGAFYMRFDEARGKLVCRFVAGDA
                     DRERTRARIGAIGARIVAAVPGSALASDQHYRETDLAIDYCEDVAPLSALDVDRIVAL
                     MQAEGMTAKVSSIHVNGWFGDYDKLAMTRILMREAFALDLDAARSTCVFAGDSPNDAP
                     MFAYFPNAVGVANLRRFLDRIVTPPAWITTREAGAGFAELADLLLASD"
     gene            complement(7591..8355)
                     /locus_tag="VFO53_08865"
     CDS             complement(7591..8355)
                     /locus_tag="VFO53_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HET8876424.1"
                     /translation="MYCATCGQAVPEGATICGNCGTSTAAKAAETASAASPPGVGAVP
                     PPPAYGGGPSIDAGAGTTLVNRIVERAKAIVLTPRTEWPVIADEATTAQDIYVRYVAP
                     LVGVGVLASLIGGVLIGIPVPLLGTVRLGIGAALGGAILHFVLTFVAIFVVTMIVDAL
                     APTFGGQKDALRALKVTAYSFTPAWVAAILTIVPALGFIAALLGLYGLYLMYLGLPVL
                     MRAPADKALGYTVVVVLCAIVLNVVVAVLTGLVTGG"
     gene            8725..8958
                     /locus_tag="VFO53_08870"
     CDS             8725..8958
                     /locus_tag="VFO53_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876425.1"
                     /translation="MIRYGFTIRTRMGQRIDNVSIIAGSQRDAERRLRQMYVQCEIVE
                     CRERSIPRRFEAANFGNMIDLMSISPPTIQHHG"
     gene            9101..10471
                     /gene="glmU"
                     /locus_tag="VFO53_08875"
     CDS             9101..10471
                     /gene="glmU"
                     /locus_tag="VFO53_08875"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET8876426.1"
                     /translation="MASPLDVVILAAGQGKRMHSALPKVLHTLAGKPLAAYVLTAARA
                     LSPRAIAVVVGHDADSVERALAAPDVSFVRQDPPRGTGDAARLALDALPLDGVTLVGL
                     GDVPLVPSEALASVAEIAQASRVGLLTARVRNPFGLGRIVRGPAGDVRAIVEERDATP
                     EERAVNEINTGFIAAPTALLKAWLSRLSPHNAQGEFYLTDIVAMAVAEGVGIGAHVVN
                     DEVSVLGVNDRAQLAKLERILQRRKATTLMLAGTTIADPDRIDIRGDLECGRDVTIDV
                     GCVFEGSVRLGERAHVGPYCVLRDCAVGDDTRVDAFCHVDSATIGRHCRIGPYARLRP
                     GTELAEEVHIGNFVEVKASTLGAGSKANHLAYVGDAVVGSRVNYGAGSITANYDGANK
                     HRSIIGDDVHVGSNCVLVAPITIGAGATIGGGSTVVKEAPAGQLTLARAAQVSNPRWK
                     RPRKKG"
     gene            10479..12317
                     /gene="glmS"
                     /locus_tag="VFO53_08880"
     CDS             10479..12317
                     /gene="glmS"
                     /locus_tag="VFO53_08880"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET8876427.1"
                     /translation="MCGIVGATSDRNIVPILIEGIRRLEYRGYDSTGLAVVNGGASPI
                     LERLVSTARVADLAAQADALHLKATTGISHTRWATHGAPTSDNAHPHLSGGEIAVVHN
                     GIIENYEALRESLKSRGYVFRTQTDTEVIAHLVHAHWHAEGGGDLLAAVRQAIAEFKG
                     AYAIAVISTRERGRLIGARQGSPLLVGIGEQDHFLASDASALMQVTRRVAYLEEGDVA
                     DVRRESYAIFDVQGRRVTRAVVEVQGSGDAVELGPYRHYMQKEIFEQPRAVADTLDGI
                     TSIGPALFGTKADAILRDVDSVLILACGTSYYSSLVAKQWIETLAKMPCTVEIASEYR
                     YRDSVPHDKSLVVVVSQSGETADTLAALGHAKALGHRHTLAICNVATSSMVRQAELVF
                     LTRAGAEIGVASTKAFTTQLAAQFLLALTLAKLRGLLGGADEAKYLDELRHIPAAIRG
                     ALALEPHLIGWAQHFANKQHALFLGRGLHYPIALEGALKLKEISYIHAEAYPAGELKH
                     GPLALVDAQMPVVAIAPNDALLEKLKSNLQEVRARGGELYVVADLDSHLTVSDGVHLI
                     RVAEHRGLLSPIVHVVPLQLLAYHTAVMRGTDVDKPRNLAKSVTVE"
     gene            12353..12532
                     /locus_tag="VFO53_08885"
     CDS             12353..12532
                     /locus_tag="VFO53_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876428.1"
                     /translation="MTDSSNGTQAVTIADSAARVALDLALRITQDSRLRPEQRDEKYW
                     LALYLKCHRAAHGDG"
     gene            complement(12549..13385)
                     /locus_tag="VFO53_08890"
     CDS             complement(12549..13385)
                     /locus_tag="VFO53_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HET8876429.1"
                     /translation="MTSRPASIADKRRTFHQLHASGCFVIPNPWDLGSARYLQGLGFK
                     ALATTSSGFAWSHAHADNRVTRDMVLAHLSEIVGATDVPINADFESGFARDAAGVTQS
                     VRLAVETGVAGLSIEDSTGDQAAPMYAIDVAVERIRAARRAIDATGGDTLLVGRAEGF
                     IAGRPDLAETIARLKAYASAGADCLYAPGIRTQEQIRAVVAAVAPKPVNLLIGWASDM
                     SVDDVAALGVRRISVGGALARSAWGGLMRAAKTIAEKGRFDGFADAASGTELDKLFGE
                     GG"
     gene            complement(13551..14099)
                     /locus_tag="VFO53_08895"
     CDS             complement(13551..14099)
                     /locus_tag="VFO53_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876430.1"
                     /translation="MATTQSGTSGSTSGTRASTAGGTQQGMTGQTPDTTYNLISVMYH
                     TLQGCQTYERYAHDAEQAGEQEIAQFFRDTGREFERCAQRGQQLLAQCLQQGQTGQAG
                     RAGQLNSQMNKPSGQQSQSSGSSGQQGQSVTSRGQQGSSHGSRSGSLASGGSSGSSGS
                     NMGSSGSSNMGSSGSNRGGSSR"
     gene            14377..15003
                     /locus_tag="VFO53_08900"
     CDS             14377..15003
                     /locus_tag="VFO53_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET8876431.1"
                     /translation="MNHLMNRFSPGATTQIRMDHTHALMTFHKYHVDTSPGRKRAIVE
                     TLSLALEIHATLEEEIFYPAMRSIDPDLVDKSIPEHGEMKRLIQELHQLRPEDRSYDT
                     TVMELMRDVIRHVADEETKLLPDAERVLGEQRLSELGAQMTRRRMQLSAPHAGAIAVN
                     TVRSYPGATLAFTGVLALGGILAARAFTRPAALRTLARPARWRALTAS"
     gene            complement(15106..15876)
                     /locus_tag="VFO53_08905"
     CDS             complement(15106..15876)
                     /locus_tag="VFO53_08905"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015621551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET8876432.1"
                     /translation="MRAARNLFSLDGRVALVTGASSGIGRAIATTLAGAGARVVLVAR
                     REAELRATRNAIVASGGEADLLPCDLGDRAALAACADAAPRAFGSPDIVVNCAGINIR
                     KPMLELTADDWDRTMRLNLDAPFFLSQRLVPAMIERGFGRIINIASLQSVRAFANSGA
                     YGASKGGLMQLTRAQAEAWSGRGVTANAIAPGFFATPLTAAVASDPARWQANAARTFI
                     GRNGELSDLAGTTLYLASRASDYVTGQTIFVDGGFSAG"
     gene            complement(15930..17234)
                     /locus_tag="VFO53_08910"
     CDS             complement(15930..17234)
                     /locus_tag="VFO53_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8876433.1"
                     /translation="MSEQRVAVIGAGVVGLACASYLQMQGRRVTLIDPRAPGEYCSFG
                     NAGCLSRGSCVPLGLPGTWRQVPGWLLDRNGPLHIPLRYVPHVAPWLWRFQRSTSIGR
                     VNAIADALHALLTVTLDKWRPLAARAGVPELIRQDGYAFAYESESGFAADALGRDIRR
                     RHGVAIDVLTGAAIREYDPVLSTGITHLVRLPEQGHCPNPLRLSRALAQRLRDGGARF
                     IEASATAFDLVDGKVARVLTDADTVEVDAVVVAAGAHSNALSAQLDSGVPLETERGYH
                     LMLGSPNVVPRAPIASGEGKYFVTPMEDGMRIAGTVELAGLDAPPNYARADALLEQAR
                     RLLPGLDGTSVSRWMGHRPSLPDSLPVIGRAVRASNAWLAFGHGHVGLTAATPTGEII
                     ADLIAGRVPFLDLRPFSADRFDAVAESVHRPAHATAHDGSVP"
     gene            complement(17257..19059)
                     /locus_tag="VFO53_08915"
     CDS             complement(17257..19059)
                     /locus_tag="VFO53_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876434.1"
                     /translation="MRNIPPFLALVWHTSPPMMLGEAGLRVVRALLPVVTLYVGKLII
                     DEVVLLAKTPHPGNDLGTWLDSGALDRIVSLLALEFGLAVLSDVLGRTASLLDSLLSE
                     KFSNATSLRLMEHAATLDLEDFEDSELQDSLERARRQAAGRMSLLGQLFGQAQDIVTI
                     VSFGAGLFIYAPWLIALMAIALVPAFVGEAHFNAQSYSLNYARTPERRELDYVRRTGA
                     SVETAKEVKIFGLNPFLIERYRTLATSFFEANRALALRRASLGSVLTAFGTIAYYVAY
                     AYIVWRTLRGQFTIGDLTFLAGSFRRLRNLLEDLLLGFSQVAGQALYLDDLFSFFEIR
                     PEIASPANPVAFPDPIREGFSFEDVGFRYPGATRWAVRHLDFTLHAREVLALVGENGA
                     GKTTLVKLLARLYDPDEGRILLDGRDLREYDLAQLRSNIGVIFQDFVRYHLSARDNIA
                     VGRIEARNDRERIVDAAHRSLADDVIAKLPEGYEQIIGKLFRGGVDLSGGEWQKIAIA
                     RAYMRDAQVLILDEPTAALDARAEFEVFQRFKELSRDKTAVLISHRFSSVRMADRIVV
                     LAEGTVEAIGTHDELLQQQGRYAELFELQAAGYR"
BASE COUNT         3093 a         6570 c         6529 g         3044 t
ORIGIN      
        1 gcaacaagct cgcgcagggc gaggcgcgcc tcggccccgg acgctatttc gacctcaagg
       61 cgatcaagca accgatcatc gtcttcgcgt cgatgggcga caacatcacg ccgccgcagc
      121 aggcgttcaa ctggatcagc gatctctact cgagcaccga ggagatcaag gccaacgggc
      181 agacgatcgt cgggctcatc catgaggacg tcgggcatct cggcattttc gtctcgggca
      241 aggtcgcgaa gaaggagcac acgcagatcg tgtcggtgct caagtacatc caggcgctgc
      301 cgcccggact gtacggcatg gagatcgctg aacggatcgg cgcggacggc aacgtcgaat
      361 acgacgtcac gctgcatgaa cgcaggctcg aggacctgaa gcgcctgcag aaatacgatc
      421 gcgtcgatga aaagccgttc gaggcggttg cggcactctc cgagctgaac gagcgcgcgt
      481 acgaattgct cgtgcgtccg ctcgtgcgcg aggcgatgcc cgaatggctc ggcaaggtgc
      541 tgcgcgaatg gcatccattg cgcgtgcagc gctggtggtt ttccgatcgc aacccgctct
      601 ttgccggctt gcccgcgatg gccgcggccg cgcgcgccca tcggcgcccc cgctcggcgg
      661 acaacctcgg acggcgcacc gagcagcttg cgtccgggta cgtgaccgcg gcactcgatc
      721 tctaccgcga tctgcgtgat gccgcaagcg aggccgcgtt ctacgagatg tacggcaacc
      781 tgctgtcgct ctcgatggcc gatcagcgcg cgatgattcg ccgccagacg ccgttcgacg
      841 cgcgcgcgtt gccggccgtg cgccaggtgc tcgacgaaat cgaccagggc ggactgcccg
      901 aggcgatcat ccgcacgggc atcctgatcg tgaaggcggg tggcggcaag cggcggctcg
      961 cgcaaatgga gcacacgcgc gcgctgttgc agcccacggg cgtgctgcgc gacatcgacg
     1021 aggatcattt ccgccgcctg ctgcacgagg agacgctggt cgtcgagttc gagccgaagc
     1081 ttgcgaaggc gtcgctgccg aagctgatgc acaacgcgtc cgatcgccag aagctcaacg
     1141 cgatcttcga ctggctcgaa accgaggcgg gcctcgatgc acgccagcaa acgctccttg
     1201 gcgacctgcg caagctcgtc ccgcgccccg cggcggcact gccgcgcgcg cggcaaacgc
     1261 cgaagggcaa ggcgagcggc cggcgcgccg cgatcgcaac ggcggagtag gatcgcctca
     1321 tggccaacgc atcggaatcc ggtgtgcgca tcgagaagga cgcgctcggc gaagtgcgcg
     1381 ttccggccga gcacctgtgg ggcgcgcaga cgcagcgctc gatcgagaac ttcccgatcg
     1441 gcgtcgagcg tttccgctgg gggcgaccgg tcatccgggc gttcggcgta ctcaagaaag
     1501 cggccgcgct cgcgaacggc gagctcggcg agctggcgcc cgacaaggtc gcgctgatcg
     1561 cacgcgccgc gcaggaagtg gtgaacggca cgctcgacgg cgagttcccg ctggtcgtct
     1621 tccagaccgg gtccggcacg cagtcgaaca tgaacgcgaa cgaggtgatc gccaaccgcg
     1681 cgatccagct cgcgggcggc gccgtcggct cgaagaagcc gatccatccg aacgacgacg
     1741 tcaaccgcgg gcagtcgtcg aacgacacgt tcccggcggt gatgcacgtc gcgacggtct
     1801 tcgagctcga ccgccgcctg ctcccggcgc tcgagcgcct gcgcacgacg ctcgacgcca
     1861 aggcgcatgc gttcgcggag gtcgtgatga tcgggcgtac gcacctgcag gacgcgacgc
     1921 cggtgacgct cggacagatc gtttccggct gggtggcgca gctcgacgat gcgcgcacgc
     1981 tcatcgagtt cacgcgcagc ggcctgatgc cgcttgcgct cggcgggacg gccgtcggca
     2041 ctggcctcaa cgcacatccc cggttcggcg cgacggtggc ggcggtaatc gcacgcgaaa
     2101 ccggcgtgcc gttcgtgtcc gcgccgaaca agttcgccgc gctttcggcg cacgacgcga
     2161 tcatcaacgt cagcgccgcg ctgcgtacgc tggcggcggc gctgatgaag atcgcgaacg
     2221 acgtccgctg gcatgcgagc gggccgcgtg caggccttgg cgaaatcacg attcccgaca
     2281 acgaaccggg gtcgtcgatc atgccgggca agatcaaccc aacgcaatgc gaggcggtta
     2341 cgatggtcgc catgcgcgtt tacgggaacg acacggcagt cgcgttcgcg ggcagccagg
     2401 gcaacttcca gctcaacgtt tacaagccgg tgatgctgca gggcacgctc gaatcggtcg
     2461 agctcctcgc cgatgcgatc gactcgttcg accagcgctg cgcgcagggt atcgagcccg
     2521 cgatggagcg cattcgcgcc aatctcgatc ggtcgctgat gctcgtcacg gcgctcaatc
     2581 cgcacatcgg ctacgagaat gcggcgaaga tcgcgctcgc cgcgcatcgc gagcattcga
     2641 cgctgcgcga ggcggcgttg aagctcggtc tcgtcagcgc cgaggatttc gaccgctggg
     2701 tcgacccggc ggcgatgacg cgcccgggtt gatgcgcatc gttgcgctga ccttcggcac
     2761 cgaaggcgac acgcgaccgc tggcggcact ttgccgagga ctgctcgacg cgggccacga
     2821 cgtcctgctg ctcggcgagc gatcgggcca cgcgtacgcc gacgcgctgc acgttccgtt
     2881 cgtggcactc gcaggcgaca tgaccgccga gctgcgagcg gcgcccggca cgctgcatcg
     2941 cggcgacgag ttcaggcacg tggcgcgcgc gcttgcggcg atcgccacgc gccacaccgc
     3001 gttgtggatg cgcgaaacgc tcgcgcgggc gaagaacgcc gatgcgatcg tctgtgcggg
     3061 actcgcgatc tatgtcggtc tttcctgcgc cgaatgcctg ggcatcccgg cgatcggcgc
     3121 cggcctgcaa ccgatgatgc cgacgcgcgc cttcgcgtcg ccgttcctgc cgccgcggcg
     3181 gctgcccggt ttcgtcaatt gggtcagcca tcgcctcgtt ctcgcgatga tgtggcgggc
     3241 gtttcgcaag gccatcaacg acgcgcggcg cgacgtcgcg caccagccgt cccggcgccg
     3301 ggagtggaac ggctatccga tcctgttcgc catctcgccg acgctggtcc cacgtccgcg
     3361 cgattggccc gaacgcgtct cggtcaccgg ctactggtgg gcgcggtccg atccgcattt
     3421 cgtgcccgac gccgccctcg aggcgtttct cgcggccggc gaagcgccgg tgtatgtcgg
     3481 cttcggcagc atggccggct tcgatcgcga ccggcttctc gccgatgtgc tgggcgcact
     3541 cgacgggcgg cgcgcgctgc ttcatgcggg ctggagcggc ttcggcgaag gacggctgcc
     3601 gggccatgtg caccggatcg gacacgtgcc gcatgactgg ctctttccgc gcgtcggcat
     3661 cgtcgtccac catggcggct cgggaacgac gcacgcggcg gcgcgcgcgg gtgtgccttc
     3721 cgtcgtcgtg cccttcgccg cggaccagtt cttctgggcc gaccgcctgc accgcgtcgg
     3781 catcgcccct gccggtctcg cctatcgcga cgtgacgccg ttgcgcctgc gcgagcggct
     3841 cgccaaggcg agcgggaacg gcatgcgcga acgcgcgaac gagatcgggc gttgcgtcgc
     3901 ggccgaacgc ggcgtcgcga acgccgtcgc gcgcatcgac gcactgctgt cgccccgccg
     3961 cgccgcgcag caggccgcgt gacggctacg cgcgcttctt cggcagttcc cgttccgcga
     4021 gcgtcaccca atagctcgcc ccgatcggca gcaccgcgtc gttgaagtcg tagtgcgggt
     4081 tgtggatgtt cggcgtgtcg gggccgcggc ccgcgccgag ccagacgtag cacccgggct
     4141 tttgctgcag catgaacgcg aaatcctcgc tgcccatctc cggcgtcgga ttggtatcga
     4201 cgttgtcgat gccgacgacg gccgccgcgg catcgcgcgc acgcgccgtc tcgtcttcgc
     4261 tgttgacggt cgccggatag cggcgctcgt agcggaggct tgcgcgcatc tcgaacattg
     4321 ccgcaatgcc gtcgacgagc gtgcgcatcc gccgctcgat cagatcctgc acgccgggct
     4381 cgaacgtgcg cacggtgccg cgcagcacga tcgtttcggg gatcacgttc cacgtgtcgc
     4441 ctgcgtgcac ctgggtgacg ctgacgacgc cggcatcctg cgggtggagg ttgcgcgaca
     4501 cgatcgtctg cagcgcgtgg atcgcatgcg cggcgaacag catcgggtcc ttgccctggt
     4561 aagccatcgc cgcgtgcgcg cccttgccgg tcgcgacgat ttcgaatatg tcgtacgcgc
     4621 ccatcagcgg ccctgcacgc atcgcgaacg taccggcggg tagtcgcggc cagttgtgca
     4681 tcccgtacac ggcgcgcatc gggaagcgat cgaacaggcc ctcctccacc atcacgcggc
     4741 cgccgccctc gttttcctcc gccggctgga agatgaaatg cagcgtgccg tcgaagttgc
     4801 gccgcgcggc gagcgccttc gcggccccga gcagcatcgt cgtatgcccg tcgtgaccgc
     4861 acgcatgcat gcggcccggg ttttgcgaag cgtgcgccac gcccgatcgc tcgtcgatgg
     4921 gcagcgcatc gagatcggcg cgaaggccga tggccgcctg cgcgcgcccg gcgcgaagca
     4981 cgccgacgac ccccgtcttc gcaagcccgg tgtacacttc gaggccgaat gcgcgcagct
     5041 tttccgcgac gaaggcgctc gtcgcctcct cctcgaacgc cgtttccggg tgcgcgtgca
     5101 ggtgatggcg ccacgcctgc atctcggcgt cgagcgcggt gatctcgggg atgagctcca
     5161 tgggcggcag cgtgaaacgg ggaagccgat cctacccgat cgtgcgcgag gtggcgcacg
     5221 gccgcgcagc gtcgatccgg tagaatcgcg cctcccgcca atggagacgc gtcccttcga
     5281 cctgctcgtg atcggcggcg gcatcaacgg cgccggcatc gcgcgtgacg ccgccgggcg
     5341 cggcctctcg gtcgtgctcg tcgagaagga cgatctcgcg gcgcacacgt cgtcggccag
     5401 cacgaagctg atccacggcg gcctgcgcta tctcgagcat tacgagttcc gcctcgtcgc
     5461 cgaggcgctc gccgagcgcg aggtgctgtt gcgcgccgca ccgcacctca tcgagccgct
     5521 gccattcgtg ctgccgcacg agccgcacct gcgtccgtcc tggatgatcc gcgcgggcct
     5581 tttgatgtac gaccggctcg gtggacggat gacgctgccg aagtcgttca gcgtgcggct
     5641 cgacggcacg cgctacggcg ccggcctcaa gccacgcttt cgcaagggct tcgtctatgc
     5701 cgatgcgcgc gtcgacgacg cgcggctcgt cgtgtgcaat gcgctcgatg cgcatgcgca
     5761 cggcgccgac atccgcgtgc gcacgaagct cgccgcggcg cggcgcgaca acggtctgtg
     5821 gcatgcgacg ctcgtcgatg ccgccggtgc gtcgtccggc atcaccgcgc gagcgctcgt
     5881 caacgccgcg ggaccgtggg tgcgcgcggt gctcggcgag ttgaccgacg ttccggtcaa
     5941 ggcgggagtt cgccacgtga agggcagcca catcgtcgtg ccgcgcattc acgatgcgcc
     6001 gcacgcctac atcctgcaga atgccgacaa ccgcatcgtc ttcgtgattc cctaccagga
     6061 tgactattcg ctgatcggaa cgacggatgt gccggtcgag cactacgcgc atccggtgat
     6121 aagcgacgag gagacgtact acctgctgac gctcgccaac acgtatctcg agcggccgct
     6181 cgcgccgagc gatatcgtct ggtcgtacag cggcgtgcgt ccgctctacg acgacggcac
     6241 cagcgacccg tcggcgatca cgcgcgatta cgtgctgcag ctcgacaccg cccgcgacgc
     6301 cgcgaacgcg ccggtgctgt cgattttcgg cggcaagatc acgacatatc gcaagctcgc
     6361 cgaagcggcg ctcgccgagt tgcgcccgtt ctttccggcg atgggctcag cgtggacgca
     6421 tgcggcgccg ctgcccggcg gcgacattcc ggacggcgat cgggacggat ggctcgcgga
     6481 gctgcgccgg cgctaccccg ccctgccggc cgacctgttg cgtgcgctcg cccgtcggca
     6541 cgggacccgc gcgctggccg ttctcgccga cgcgcgctcg cccgccgacc ttggcgaaga
     6601 cttcggttcg caacttaccg agcgcgaaat acgctacctg cagcgcgacg aatgggccgc
     6661 gaccgccgag gacgtgctgt ggcggcgcac gaaatgcggg ctagcgatga cgcgcgaagc
     6721 gtgtgcacgc gtcggcgagt acctcggcca atgagcaaag cgatgccgcc gcgtccgcta
     6781 gcgcagatgc cgctcgcggc tcggcgcgcg atccgtttcg tgctgaccga catcgacgac
     6841 acgctcacga ccgacggcaa gctgacggtc gaggcgtacg cggcgctggc gcggctttct
     6901 gcgagcggcc gcattgtcgt tcccgtgacc ggccggccgg cgggctggtg cgatcacatc
     6961 gcgcgaatgt ggccggtgga tgcggtggtc ggcgagaacg gcgcgttcta catgcgcttc
     7021 gacgaagcgc gcggaaagct cgtgtgccgg ttcgtcgcgg gcgacgccga ccgcgaacgg
     7081 acgcgtgcac ggatcggcgc aatcggtgcg cggatcgtcg cggcggtgcc gggcagcgca
     7141 ttggcgtcgg atcagcatta tcgcgagacc gatcttgcga tcgactattg cgaggacgtg
     7201 gcgccgcttt ccgcgctcga cgtcgaccgg atcgtggcgc tgatgcaggc cgagggcatg
     7261 acggcgaagg tgagctcgat ccacgtcaac ggctggttcg gcgattacga caagctcgcg
     7321 atgacgagga tactgatgcg cgaagcgttc gcgctcgatc tcgatgccgc gcgcagcacc
     7381 tgcgtgttcg ccggcgattc gccgaacgac gcgccgatgt tcgcatactt cccgaatgcc
     7441 gtcggcgtcg ccaacctgcg gcgatttctc gaccgcatcg tcacgccgcc cgcatggatc
     7501 accacgcgcg aagcgggtgc cgggttcgcg gagcttgccg acctgttgct ggccagcgac
     7561 tgaggccgat gcacgtcacg cgtgcgcgcc ctacccgccg gtaacgagac ccgtcagcac
     7621 ggcgacgacg acgttgagca cgatcgcgca cagcacgacg acgacggtgt agccgagcgc
     7681 cttgtccgcg ggtgcgcgca tcagcaccgg caatccgagg tacatgaggt aaagcccgta
     7741 gagaccgagc agcgcggcga tgaagccgag cgcgggcacg atcgtcagga tcgccgccac
     7801 ccacgcgggc gtgaagctgt acgcggtgac cttcaatgcg cgaagcgcat ccttctggcc
     7861 gccgaaggtc ggcgcgagtg catcgacgat catcgtgacg acgaagatcg caacgaacgt
     7921 cagcacgaag tgcaggatcg ccccgccgag cgccgcaccg atgccgagac gcaccgtgcc
     7981 gagcagcggc acgggaatgc cgatcagcac gccgccgatc aacgacgcca gcacgccgac
     8041 gcccaccagc ggtgcaacgt agcggacata gatgtcctgc gcggtcgtcg cctcgtcggc
     8101 gatcaccggc cactcggtgc gcggcgtcag cacgatggcc ttggcgcgct cgacgatgcg
     8161 gttgacgagc gtcgttcccg cgcccgcatc gatgctcggg ccgccgccat aggccggtgg
     8221 cgggggcacc gcgccgacgc ccggaggcga cgccgcggat gcggtctcgg ccgccttcgc
     8281 cgcggtcgac gtcccgcaat tgccgcagat cgtcgcgcct tccggaactg cctgcccgca
     8341 tgtcgcgcaa tacatcgcgt tcctcccgtg tcggctgccg tctggcgctc ggattgtgca
     8401 atacctgccc cgccgcgaca acgcatacgg tcgctttgag tcacgcggcg tccatgcaga
     8461 agcgacacga agcgttcgcc ggacgggcaa gggcgccgcg ccggggtgaa tcgacggaag
     8521 gttcgaagtg ggcgctcgct cgcttcgacg gccgcttaaa cgctacaggg tgcctgaacc
     8581 agacgcgcgc cgatgcagcc tctatccagt ccaccgcagc gttcgcgcat cgtggggaac
     8641 ggtatttgcc ttatgcgact cccggacgtc gtaatccact gattcgcata ggacgccggc
     8701 cacgaaacag gaggcagagc aaccatgatc cgctacggct tcacgatccg cacccggatg
     8761 ggacagcgga tcgacaacgt ttcgatcatc gccggatcgc agcgcgacgc cgagcgtcgc
     8821 ctgcgccaga tgtacgtcca gtgcgagatc gtcgagtgcc gcgagcgctc gattccccgg
     8881 cgcttcgagg cggcgaactt cggcaacatg atcgacctga tgtcgatcag tcctccgacg
     8941 atccagcacc acggctgact gccgcggtgc ccgcgccggc gctacgctgc cggtgctgca
     9001 ccggatggcc gcgtcgcgac cgccggtcgg ccgccgcgcg gaaaagtcga catggtgcac
     9061 tggcgtatca tcgccgtcgt ccccgcgttt cttccgttcg atggcttccc cgctcgatgt
     9121 cgtcatcctc gccgccggcc aaggcaagcg aatgcattcg gcgttgccga aggtgctgca
     9181 caccctggct ggaaagccgc tcgctgcgta cgtcctgacg gctgcgcggg cgctgtcacc
     9241 gcgcgcgatc gcggtcgtcg tcggccatga cgcggacagc gtcgagcgcg cgcttgccgc
     9301 gcccgatgtc agcttcgtgc gccaggatcc gccgcgcggc acgggcgatg ccgcacgcct
     9361 cgcgctcgac gcgctgccgc tcgacggcgt gacgctcgtc gggctcggcg acgtgccgct
     9421 cgtcccgtcc gaagcccttg caagcgtcgc cgaaatcgcg caggcaagcc gcgttggcct
     9481 cctcacggcg cgcgtgcgca acccgttcgg actggggcgc atcgtgcgcg ggcccgccgg
     9541 cgatgtgcgg gcgatcgtcg aggagcgcga tgcaacgccc gaggagcgcg cggtcaacga
     9601 gatcaacacc ggattcatcg cggcgccgac cgcgcttttg aaggcctggc tgtcgaggct
     9661 gtcgccgcac aatgcgcagg gcgagttcta cctcaccgat atcgtggcga tggcggttgc
     9721 cgagggcgtc ggcatcggcg cgcacgtcgt caacgacgaa gtgtcggtcc tcggcgtcaa
     9781 cgatcgtgcc caactcgcga agctcgagcg catcctgcag cggcgcaagg ccacgacact
     9841 gatgcttgcc gggacgacca tcgccgaccc cgaccgcatc gacatccgcg gcgatctcga
     9901 atgcgggcgc gacgtgacga tcgacgtcgg ttgcgtcttc gagggcagcg tgcggctcgg
     9961 cgagcgtgcg cacgtcggtc cgtattgcgt gctgcgcgac tgcgcggtgg gcgacgatac
    10021 gcgtgtcgat gcgttctgcc acgtcgattc ggcgacgata ggccggcatt gccgcatcgg
    10081 tccgtatgcg cgactgcgcc ccggcacgga gcttgctgaa gaggtgcaca tcggaaactt
    10141 cgtcgaagtg aaggcgagca cgctcggcgc cggctccaag gcgaatcatc tggcgtacgt
    10201 cggcgacgcg gtcgtcggca gccgcgtcaa ttacggcgcc ggctcgatca ccgcgaacta
    10261 cgacggcgcg aacaagcatc ggtcgatcat cggcgacgac gtgcacgtcg gctccaactg
    10321 cgtgctcgtc gcgccgatca cgatcggcgc cggggcgacg atcggcggcg gcagcaccgt
    10381 cgtgaaggaa gcgccggccg gacaactgac gctggcacgc gccgcgcagg tttcgaatcc
    10441 gcgctggaag cggccgcgga agaaggggtg agctgatcat gtgcgggatt gtcggcgcca
    10501 cgtccgatcg caacatcgtg ccgatcctca tcgaaggcat ccggcgcctc gagtaccgcg
    10561 gctacgattc gaccgggctt gcggtcgtga acggcggcgc aagcccgatt ctcgagcggc
    10621 tcgtttcgac ggcacgggtt gccgacctcg cggcacaggc cgacgcgttg catctcaagg
    10681 ccacgacggg catctcgcac acgcgctggg ccacgcacgg ggcgccgacg tccgacaatg
    10741 cgcacccgca cctgtccggc ggcgagatcg ccgtcgtcca caacggcatc atcgagaatt
    10801 acgaagcgct gcgcgagagc ctgaaatcgc gcggctacgt gttccgcacg caaaccgaca
    10861 ccgaggtgat tgcgcacctc gtgcatgcgc attggcacgc cgaaggcggt ggtgatctgc
    10921 tcgcggccgt ccgccaggcg atcgccgaat tcaagggcgc gtacgcgatc gccgtgatct
    10981 cgacgcgcga gcgcggccgt ctgatcggcg cgcggcaggg cagcccgctc ctggtgggaa
    11041 tcggcgagca ggatcacttc ctcgcatccg acgcatcggc gctgatgcag gtgacacgcc
    11101 gcgtcgcgta tctcgaggag ggcgacgtcg ccgacgtgcg gcgcgaatcg tacgcgatct
    11161 tcgatgtgca aggacgccgc gtgacgcgtg ccgtcgtcga ggttcaggga agcggcgacg
    11221 cggtcgagct cgggccgtac cggcactaca tgcagaagga gatcttcgag cagccgcgcg
    11281 cggttgccga tacgctcgac ggcatcacgt cgatcggtcc tgcactcttc ggcacgaagg
    11341 ccgacgcgat ccttcgcgac gtcgattcgg tgctgatcct cgcctgcggc accagctact
    11401 actcgagcct cgtcgcaaag cagtggatcg agacgctcgc gaagatgcca tgcacggtgg
    11461 aaatcgccag cgaatatcgc tatcgcgaca gcgttccgca cgacaagtcg ctcgtcgtcg
    11521 tcgtgtcgca atcgggcgag acggccgata cgctcgcggc cctcggacac gccaaagcgc
    11581 tcggccaccg ccacacgctt gccatctgca acgtcgcgac gagctcgatg gtgcggcagg
    11641 ccgagctcgt gttcctgacg cgcgccggcg ccgagatcgg cgtcgcgtcg acgaaggctt
    11701 tcaccacgca gctcgccgcg cagttcctgc tcgcgctgac gctcgcgaag ctgcgcgggc
    11761 tcctcggtgg cgccgacgaa gcgaagtatc tcgacgagct gcggcatatt ccggcggcca
    11821 tccgcggcgc gctcgcgctc gagccgcacc tgatcggctg ggcgcagcat ttcgcgaaca
    11881 agcagcacgc gctcttcctt ggacgcgggc tgcactaccc gatcgcgctg gaaggcgcgc
    11941 tcaagctcaa ggaaatctcg tacatccacg ccgaagccta tccggcaggc gagctgaagc
    12001 acggcccgct cgcgctggtc gacgcgcaga tgccggtcgt cgcgattgcg ccgaacgacg
    12061 cgctgctcga gaagctcaag tcgaacctgc aggaggtgcg cgcgcgcggc ggcgagcttt
    12121 acgtcgtcgc cgatctcgac agccacctca cggtgagcga cggcgttcat ctgatccgcg
    12181 tcgccgagca ccgtgggctg ttatcgccga tcgtgcacgt cgtgccgctg cagcttctcg
    12241 cgtatcacac ggcggtgatg cggggcaccg acgtcgacaa gccgcgcaat ctcgcgaaat
    12301 cggtgacagt cgagtagccg ccgctcgccg tccgcgatca ccaggaaaat tcatgacaga
    12361 cagcagcaac ggaacacagg ccgtcaccat tgccgacagc gccgcacgcg tggcgctcga
    12421 tctggcgttg cgaatcacgc aggactcgcg cttgcgcccg gagcagcgcg acgaaaagta
    12481 ctggcttgcg ctgtacctga agtgccatcg cgcagcgcac ggcgacggct gaagctcgcg
    12541 tctatcgctt acccgccttc gccgaacagc ttgtcgagct cggtgcccga ggcggcgtcg
    12601 gcgaagccat cgaagcggcc cttctcggca atcgttttgg ccgcgcgcat caggccgccc
    12661 cacgcgctcc gcgcgagcgc accgccgacg ctgatgcggc gaacgccgag tgccgcgacg
    12721 tcgtcgacgc tcatgtcgct tgcccagccg atcagcagat tcacgggctt cggcgcaacg
    12781 gctgcgacca ccgcacggat ctgctcctgc gtgcggatgc ccggcgcata caggcagtcg
    12841 gcgccggcgc tcgcgtacgc cttgaggcgt gcgatggttt cggcgaggtc gggacggccg
    12901 gcgatgaagc cttcggcgcg tcctaccagc agcgtatcgc cacccgtggc gtcgatcgca
    12961 cggcgtgctg cgcggattcg ctcgaccgcc acatcgatcg cgtacatcgg tgccgcctgg
    13021 tcgccggtcg aatcctcgat cgacaatccg gcgacgccgg tctccacggc aaggcgcacg
    13081 ctttgggtca cgccggcagc gtcgcgggca aagccgcttt cgaaatcggc gttgatcggc
    13141 acgtcggtcg cgccgacgat ttcgctcaag tgcgcgagca ccatgtcgcg cgtgacgcga
    13201 ttgtcggcgt gagcgtgcga ccaggcgaag ccggagctcg tcgtcgcaag cgccttgaag
    13261 ccgaggcctt gcaggtagcg cgcgctgccg agatcccagg gattggggat gacgaaacat
    13321 ccgcttgcgt gcaactgatg gaacgtgcgc cgcttgtcgg cgatgctggc aggacgagag
    13381 gtcatcggcg attttccgaa gcgtcggatg aaagcgacgc cgctcgattc taccgctgcc
    13441 gattgcgatc cgccgattcg ggaatgccgg ttcgcgcgag cgattgctta tgtgccaaag
    13501 ggaaggggcg gaaatttctc cgccccttgc atcgccatcg gcgtcgccgc ttaccggctg
    13561 ctgccgcccc tgttgctgcc gctgctgccc atgttcgagc tgccgctgct gcccatgttc
    13621 gagccgctac tgccgctgct tccaccgctg gcgagactgc cgctgcggct tccgtggctg
    13681 ctgccctgct gtccgcgcga cgtgaccgac tggccctgct ggcccgacga gccgctcgac
    13741 tggctctgct gccccgaggg cttgttcatc tggctgttga gctggcccgc gcggcccgct
    13801 tgccccgttt gtccctgctg caggcactgc gcgagcaact gctggccgcg ctgcgcgcag
    13861 cgttcgaact cgcggccggt atcgcggaag aactgtgcga tctcctgctc ccccgcctgc
    13921 tcggcatcgt gcgcgtatcg ctcgtacgtc tggcacccct gcagcgtgtg atacatgacg
    13981 ctgatcaggt tgtatgtcgt gtcgggcgtc tgtcccgtca tgccctgctg cgtgccgccc
    14041 gccgtcgagg cgcgcgttcc gctcgtggag cccgacgttc ccgattgtgt cgttgccatg
    14101 tgcgttactc cttccgtgat gcccgctgat gggtgatcga agaccgcgct tgtatgaacg
    14161 agcgccgccc tgccgtccgg tcggaccgtc ggcggacggt ggagtttcgc cgtgcgcgtt
    14221 ccgctttgcg cccgtgcctg ccgcagccgg attgttgcgc gcttcaacac gttcgcggtg
    14281 ccgatgcgct cgccgagcgg ccagcgaact tgcgcgcgac atcaaggtcc gcatcatgtc
    14341 taaggtcaat acgaccggcc acgcatggag acgcgcatga accacctgat gaatcgattc
    14401 tcgcctggcg ccaccaccca gatccgcatg gatcacacgc atgcgctgat gacattccac
    14461 aagtaccacg tcgacacgtc gccgggcagg aaacgcgcga tcgtcgaaac gctgtcgctc
    14521 gccctcgaaa tacatgcgac gctggaagag gaaatcttct acccggcaat gcgcagcatc
    14581 gaccccgacc tcgtcgacaa gagcattccc gagcacggcg agatgaagcg gctgatccag
    14641 gagctgcatc aattgcggcc cgaagatcgc agctacgaca cgacggtgat ggaactcatg
    14701 cgcgatgtca tccggcacgt tgcggacgag gaaaccaagc ttcttcccga tgccgagcgg
    14761 gtgctgggcg agcagcggct ctccgagctc ggggcgcaga tgactcgtcg gcgcatgcag
    14821 ctctccgcgc cgcacgccgg cgcgatcgcc gtcaacaccg tgcgctcgta cccgggtgca
    14881 acactcgcgt tcaccggggt gctcgcgctg ggtggcattc tcgcggcacg cgcgttcacc
    14941 cgcccggccg ccttgcgcac gctcgcgcgg ccggcgcgct ggcgcgcatt gaccgcgtcg
    15001 tagcggaatc catccgatcg cgacatcgtc cgccgcgcag cggcggcggg ccgcgcaagc
    15061 actaccggtc gatcgtcggc cggagtgcgc gccggccgca cgcggtcaac cggcggaaaa
    15121 cccgccgtcg acgaagatcg tctgtcccgt gacgtaatcc gatgcgcggc ttgcgaggta
    15181 gagcgttgtt ccggcaaggt cggacaattc gccgttgcgc ccgatgaacg tgcgcgctgc
    15241 gttcgcctgc cacctggcgg gatcggatgc aacggcggcg gtgagtggcg tcgcgaaaaa
    15301 tccgggcgcg atcgcgttgg cggtaacgcc ccggccagac cacgcttccg cctgcgcgcg
    15361 cgtcaattgc atcagcccgc ccttcgacgc gccgtacgcg ccgctgttcg cgaacgcgcg
    15421 caccgactgc agcgaagcga tgttgatgat ccggccgaag ccgcgctcga tcatcgccgg
    15481 cacgagccgc tgcgaaagga agaagggcgc gtcgaggttg agccgcatcg tgcgatccca
    15541 gtcgtcggcg gtgagctcga gcatgggctt gcggatgttg atgccggcgc agttcacgac
    15601 gatgtccggg gaaccaaagg cgcggggcgc cgcatcggcg catgccgcga gcgcggcccg
    15661 atcgcccagg tcgcagggca gcaggtcggc ctcaccgccg ctcgcgacga tggcattgcg
    15721 cgtcgcgcgt agctccgctt cgcgccgcgc gacgagcacg acgcgggcgc ccgcaccggc
    15781 gagcgtggtc gcgatcgcgc ggccgatgcc ggagcttgcg ccggtgacga gcgcgacgcg
    15841 tccatccagc gagaacagat ttcgggctgc ccgcaagctt tccgcggctg ctgcgccgtt
    15901 ttcgctgccc ggcgcttgct tgcaattcgt catggaacac tcccgtcgtg cgcggtggcg
    15961 tgcgccggtc gatgcacgct ttctgccacg gcgtcgaagc gatcggccga aaacggccgc
    16021 aggtcgagaa acggtacgcg cccggcgatg aggtccgcaa tgatctcgcc ggtaggtgtt
    16081 gccgcagtca atccgacgtg gccgtgtccg aacgcgagcc acgcattcga cgcgcgcacg
    16141 gcacggccga tcacgggcag cgaatcgggc agcgaaggcc gatggcccat ccagcgcgat
    16201 acgctcgtgc cgtccagccc gggcagcagc cggcgcgcct gctcgagcag cgcatcggcg
    16261 cgcgcgtagt ttggcggcgc atcgaggccc gcgagctcga cggtgcctgc gatgcgcatc
    16321 ccgtcctcca tcggcgtcac gaaatatttg ccctcgcccg atgcaatcgg cgcgcgtgga
    16381 acgacgtttg gcgagccgag catcaggtgg tagccgcgct cggtctcgag cggaacgccg
    16441 ctgtcgagct gcgccgataa cgcgttcgaa tgtgcgccgg cggcaacgac gaccgcgtcg
    16501 acctccacgg tgtccgcatc ggtgagcacg cgcgccacct tgccgtcgac gagatcgaag
    16561 gcggtcgcgg acgcctcgat gaacctggcg ccgccatcgc gcagccgctg cgcgagcgcg
    16621 cgcgacaagc gcagcggatt ggggcaatgg ccctgctcgg gcaggcgcac gagatgggta
    16681 atgccggtgg acagcaccgg gtcgtattcg cggatcgcag cgccggtcag cacgtcgatc
    16741 gcgacgccgt ggcgacgccg gatgtcgcga cccagcgcgt cggccgcaaa gccggattcg
    16801 ctttcgtagg cgaacgcgta gccatcctgc cggatcagct cggggacgcc ggcccgtgcc
    16861 gcgagcggcc gccatttgtc gagcgtgacg gtgagaagcg cgtgcagcgc gtcggcaatc
    16921 gcgttgacgc ggccgatcga cgtcgagcgc tgaaagcgcc agagccaggg tgctacgtgc
    16981 gggacatagc gcaaaggaat gtgcagcggg ccgttgcgat cgagcagcca gcccggcacc
    17041 tgcctccacg taccgggcaa gccgagcggg acgcaggagc cacggctcaa gcacccggcg
    17101 ttgccgaacg agcagtactc gcccggcgcg cgcgggtcga tcagcgttac gcgccgcccc
    17161 tgcatctgca gatagcttgc gcacgcgagc ccgacgacgc ctgcaccgat cacggcaacg
    17221 cgttgttcgc tcaccgaagg ctccaggtcg cacgggtcat cggtacccgg ccgcctgcaa
    17281 ctcgaacaat tcggcgtagc gaccctgctg ctgcagcagc tcgtcgtgcg tgccgatcgc
    17341 ttcgacggtg ccttccgcga gcacgacgat gcgatccgcc atgcggacac tcgaaaagcg
    17401 gtgcgagatc agcacggccg tcttgtcacg tgacagttcc ttaaagcgct ggaacacctc
    17461 gaactcggcg cgcgcgtcga gcgccgcggt gggctcgtcg agaatcagca cctgcgcgtc
    17521 gcgcatgtag gcgcgggcaa tggcaatctt ctgccattcg ccgcccgaca gatcgacacc
    17581 gccgcgaaag agcttgccga tgatctgctc gtacccttcc ggcagcttcg caatgacgtc
    17641 gtcggcgagg ctgcgatgcg ccgcgtcgac gatgcgctcg cgatcgtttc gtgcctcgat
    17701 gcggcccacg gcgatgttgt cgcgcgccga caggtgatag cggacgaagt cctggaagat
    17761 cacgccgata ttcgagcgca gttgcgcgag atcgtattcc cggaggtcgc gtccgtcgag
    17821 caggatgcgc ccttcgtccg ggtcgtagag gcgcgcgagg agcttcacga gcgtcgtctt
    17881 gcccgcgccg ttctcgccga cgagcgccag cacctcgcgc gcatgcagcg tgaaatcgag
    17941 gtggcgcacg gcccagcgtg tagcacccgg gtagcgaaag ccgacgtctt cgaacgaaaa
    18001 gccttcgcgg atcggatcgg gaaacgcaac cggattggcg ggcgaagcga tctcgggccg
    18061 gatctcgaag aacgaaaaca ggtcgtcgag gtagagcgcc tggcccgcca cctgcgagaa
    18121 tccgagcagc aggtcctcga gcaggttgcg gaggcgccgg aacgatccgg cgaggaaggt
    18181 caggtcgccg atcgtgaact ggccgcgcag cgtgcgccac acgatgtacg catacgcgac
    18241 gtagtacgcg atcgtgccga acgccgtcag cacgctgccg aggctcgcgc ggcgcagtgc
    18301 gagcgcgcga ttggcctcga agaagctcgt cgcgagcgtg cggtagcgct cgatcaggaa
    18361 cggattgagg ccgaaaatct tgacctcctt ggccgtctcg acgcttgcgc cggtgcgccg
    18421 gacgtaatcg agctcgcgcc gctccggcgt gcgcgcatag ttgagcgaat agctctgcgc
    18481 attgaagtgc gcctcgccga cgaacgcggg gacgagcgcg atcgccatca gcgcgatgag
    18541 ccacggcgca tagatgaaga gcccggcgcc gaagctcacg atcgtgacga tgtcctgcgc
    18601 ctggccgaag agctggccga gcagcgacat gcgccccgcg gcctggcgcc gcgcgcgctc
    18661 gaggctgtcc tgcagctcgc tgtcctcgaa gtcctcgagg tcgagcgttg ccgcgtgctc
    18721 catcagccgc aaactggtcg cgttgctgaa tttctcggac agcagcgaat ccagcagcga
    18781 cgcggtgcgt cccaggacgt ccgaaagcac cgccagcccg aattccagcg cgagaagcga
    18841 aacgatgcga tcgagcgcgc cgctgtcgag ccaggtgccg aggtcgtttc ccggatgtgg
    18901 cgtcttcgcg agcagcacga cctcgtcgat gatgagcttg ccgacgtaga gcgtgacgac
    18961 cggcagcagc gcgcggacga cgcgcagccc ggcctcgccg agcatcatcg gcgggctcgt
    19021 gtgccagacg agggcgagga acggcgggat gttgcgcatg ccgctcatcc gctcgcgcag
    19081 cgagcgccgc tcggcatgaa tcacgcgctc ggccgcgctc gccttggggg tgctcgatcc
    19141 gatcgaactg ttgcggggca cggcgtctat tgtgccgcca gcggactttt cgctccggcg
    19201 gcggtgtcca aagtgctcga aacccgcgag cgagaa
//
LOCUS       DASSVX010000208         5355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1375390, whole genome shotgun sequence.
ACCESSION   DASSVX010000208 DASSVX010000000
VERSION     DASSVX010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375390"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1507
                     /gene="ntrC"
                     /locus_tag="VFO53_08920"
     CDS             95..1507
                     /gene="ntrC"
                     /locus_tag="VFO53_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HET8876435.1"
                     /translation="MWVVDDDRSIRWVLEKALAREAIAHRMFASAYEVRQALAISQPQ
                     VLICDIRMPGESGLALLAEVKERHPQIPIIIMTAYSDLDSAVAAFQGGAFEYLPKPFD
                     VDHALALIRRAAAEARHAPAVTPLAAGAAEILGQAPAMQEVFRAIGRLSQSTATVLIT
                     GESGTGKELVARALHRHSPRAAGPFVAINTAAIPKDLLESELFGHERGAFTGAQGTRR
                     GRFEQAEGGTLFLDEIGDMPLDLQVRLLRVLADGEYYRVGGHAVQRATVRIIAATHRD
                     LDERARHGTFREDLLHRLNVVRLRLPALRERPGDIAPLARHFLRKSASELAVEPKVLS
                     DAAMDALATFPFPGNVRQLENVCHWLTVMAPGQRIEPADLPIEIRNGARAAAEHDSGW
                     IDALDAELAHALAHAEAGVGARLQRDFERALIRRSLVFTGGHRMEAAARLGWGRNTLT
                     RKIQELGLEAELRKGGPRKR"
     gene            complement(1515..2705)
                     /locus_tag="VFO53_08925"
     CDS             complement(1515..2705)
                     /locus_tag="VFO53_08925"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876436.1"
                     /translation="MAVAADSLALPRVPPPASPQHRRRDIAAYALFVVAITAMGIGAF
                     RPQPRATLDAENRTLAAWPAWSMSRAFIAAFDRAFADRFGGREVLLRLHNRVLVRVFG
                     VSPSPDVLIGNDGWLFFKGEDGRAFDRYYRGTQPVADAELQRVVDELRRRERFLAAHG
                     IGYVVTIAPDKATIYPEHLPRWATRATQHSPLERLTGMIRAAGTLHFVDLREPLIAAK
                     LHSRVYYATDSHWNSIGARVAYDELMRSAGDFLAARLPAAADVTMPPYVAGVDVYHGD
                     LARMTGDINRFPEPDYAPLWKTLAAADTRCGKRIDSGKDPGFEWYACNRPGLPRAVIY
                     RDSMAIPLIPMLSENFSRVAYVSSHRLDPVFVLRERPDVVIEEMVERAMLAPLATPMP
                     DPGR"
     gene            complement(2705..4135)
                     /locus_tag="VFO53_08930"
     CDS             complement(2705..4135)
                     /locus_tag="VFO53_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HET8876437.1"
                     /translation="MVFASPLFLFLFLPATIVAYFALPRRFRNAVLLVASVAFYTWGE
                     ARYVPLVLGSVAFNWWIGLRIAAASEPPARKRRLAAAVAANLAALAVFKYANFAVANV
                     DAIAPIFGLSPVALRAIPLPLGISFFTFHAISYVVDVYKRTAGAQRSIREFALYILLF
                     PQLIAGPIIRYRDIAAQLVARDANVSEFAYGVRRFVLGLGKKLLIANTLGATADAIFA
                     LPGSELAAPLAWLGIACYTLQIYFDFSGYSDMAIGLMRMFGFRILENFNYPYIAGSIR
                     EFWRRWHISLSNWFRDYVYVPLGGNRAGTRRAYANLIIVFLLCGLWHGASWPFVLWGA
                     WHGLFLIVERAGLARHLERHRVVAHAYTLAVVVGGWVLFRCDSLAHALDYYRALGGLS
                     SAAPTLHPLGEYVNRLLLVTLAAAVAFATPVATRIAALRERAMHWPRAEPLVVTADGA
                     WIAAVFVAASAMLAAGTYNPFIYFRF"
     gene            complement(4136..4738)
                     /locus_tag="VFO53_08935"
     CDS             complement(4136..4738)
                     /locus_tag="VFO53_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET8876438.1"
                     /translation="MARSPIATLSVVVIAALASGALAQPLYRYVDPNGRVVYSDQPPP
                     ATAKDAQMKQLPQNVIETDPVPLQAREAAEHFPVTLYTFDCDICRDAQALLVKRGIPF
                     ETVVVSEEKGAARLKALTGKQTAPVLAIGDKQVLQGYNETLWQARLDDAGYPRTAPAA
                     RRAPATAGAAAAPRTAPDDAAAPAAAGEPARPAPGQGYPK"
     gene            complement(4756..>5355)
                     /locus_tag="VFO53_08940"
     CDS             complement(4756..>5355)
                     /locus_tag="VFO53_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET8876439.1"
                     /translation="GVSGLQGVEWDAVELPSQFMENYCWQWDVLSHMTAHAKTGAPLP
                     RELFGRMLAARNYGSGMAMLRQVELAMFDMLLHTAYVPGAGGGYPSAEAVLDAVRREV
                     AVVPKPPYDRVMHAFGHVFAGGYAAGYYSYKWAEVLSADAFSLFEEAGVLSPQVGARF
                     RDEVLARGGSRTALESFVAFRGRPPSLDALLRHNGMVAS"
BASE COUNT          920 a         1804 c         1897 g          734 t
ORIGIN      
        1 ggaaactgcg agcatcgagg gacgatcgag cgccgagggc gcaccgcaag ccgcgaccct
       61 gcatcgggcg acggcgcagg agcatgcggg caccgtgtgg gtcgtcgacg acgaccggtc
      121 gattcgctgg gtgctcgaaa aggcgctcgc gcgcgaagcg atcgcgcacc gaatgtttgc
      181 ctcggcctac gaagtgcggc aggcgcttgc gatcagccag ccgcaggtgc tgatctgcga
      241 catccgcatg ccgggggaat ccggcctggc gctgctcgcc gaagtgaagg agcggcatcc
      301 gcagattccg atcatcatca tgaccgccta ttcggatctc gatagcgcgg tggcggcgtt
      361 ccagggcggc gcattcgaat acctgccgaa gccgttcgac gtagaccacg cccttgcgct
      421 catccggcgc gcggccgccg aggctcgcca cgcgccggcg gtcacgccgc tcgccgccgg
      481 cgcggcggag attctcggcc aggcgccggc gatgcaggaa gtgtttcgcg cgatcgggcg
      541 cctgtcgcag tcgacggcga ccgtgctcat caccggcgag tcgggcaccg gcaaggagct
      601 cgtcgcgcgt gcgctgcatc ggcacagccc gcgcgccgcc gggccattcg tcgccatcaa
      661 cacggccgcg attccgaagg acctcctcga gtcggagctg ttcggccacg agcgcggcgc
      721 gttcaccggc gcgcagggca cgcggcgcgg ccgcttcgag caggccgaag gcggtacgct
      781 gttcctcgac gagatcggcg acatgccgct cgacctgcag gtgcggctgc tgcgcgtgct
      841 cgccgacggc gagtactacc gcgtgggcgg gcacgcggtg cagcgcgcga ccgtgcgcat
      901 catcgcggcg acgcatcgcg atctcgacga gcgcgcgcgg catggcacgt ttcgcgagga
      961 cctgctgcac cggctcaacg tcgtgcggct gcgtttgcct gcattgcgcg agcgtccggg
     1021 cgacatcgcg ccgctcgcac gccatttcct gcgcaagagc gcaagcgagc tcgcggtcga
     1081 gccgaaggtg ctgtccgacg cggccatgga cgcgctggca actttcccgt tcccgggcaa
     1141 cgtgcgccag ctcgagaacg tgtgccactg gctgaccgtg atggcaccgg ggcagcgcat
     1201 cgagcccgcc gacctgccga tcgaaatacg gaacggcgcg cgtgccgccg cggagcacga
     1261 cagcggctgg atcgacgcgc tcgacgccga gctcgcgcac gcgcttgcac acgccgaggc
     1321 cggggttggc gctcgcctgc agcgcgactt cgagcgcgcg ctgatccgcc gttcgctcgt
     1381 gttcaccggc ggccaccgga tggaggcggc cgcgcggctt ggctggggcc gcaacacgct
     1441 gacgcgcaag atccaggagc tcgggctcga ggcggagctg cgcaagggtg gcccgcgcaa
     1501 gcgctgaccg cgccctaccg cccggggtcg ggcatcggcg tggcgagcgg agcgagcatg
     1561 gcgcgctcga ccatttcctc gatcacgaca tcgggccgtt cacgcaggac gaacacggga
     1621 tcgagccgat ggctcgacac gtacgcgacg cggctgaaat tctcggaaag catcgggatc
     1681 agcgggatcg ccatcgagtc gcgatagatc acggcgcgcg gcagccccgg ccggttgcac
     1741 gcgtaccact cgaagcccgg gtccttgccg gaatcgatgc gcttgccgca gcgggtatcg
     1801 gccgccgcga gggtcttcca cagcggcgcg tagtcgggct cgggaaagcg attgatgtcg
     1861 cccgtcatgc gcgcaagatc gccgtgatag acatcgacgc ccgcgacgta cggcggcatc
     1921 gtcacatcgg cggctgcggg taatcgcgcc gcgaggaaat cgccggcgct gcgcatcagc
     1981 tcgtcgtagg cgacgcgcgc gccgatcgag ttccaatgcg agtcggtcgc gtagtagacg
     2041 cgcgaatgca gcttggcggc gatcagcggc tcgcggaggt cgacgaaatg caacgtgccg
     2101 gccgctcgaa tcatgccggt cagccgctcg agcggcgagt gctgcgtggc acgcgttgcc
     2161 catcgcggaa gatgctcggg atagatcgtc gccttgtcgg gggcgatcgt cacgacgtag
     2221 ccgatgccat gcgccgcgag aaagcgctcg cgtcgccgca actcgtcgac gacacgttgc
     2281 aactcggcgt cggcgacggg ctgcgtgccc cgatagtagc gatcgaacgc gcggccgtcc
     2341 tcgcccttga agaagagcca gccgtcgttg ccgatcagca cgtcaggtga aggcgagacg
     2401 ccgaatacgc gcacgagcac gcgattgtgg aggcgcagca gcacttcgcg gccgccgaag
     2461 cggtcggcga acgcgcggtc gaacgccgcg atgaacgcgc gactcatcga ccatgcgggc
     2521 catgcggcga gcgtgcgatt ttccgcgtcg agcgtcgccc gcggctgcgg acggaaggcg
     2581 ccgatcccca tcgccgtgat cgcgaccacg aacagcgcgt acgcagcgat gtcgcggcgc
     2641 cggtgctgcg gggacgccgg cggcggaacc cgcggaagcg ccagcgaatc ggccgcaacc
     2701 gccatcagaa acgaaagtag atgaacgggt tgtacgtgcc cgcggccagc atggcggacg
     2761 cggcgacgaa caccgccgcg atccaggcgc catcggcggt gacgacgagc ggttcggcgc
     2821 gcggccagtg catcgcgcgc tcccgcaagg cggcgatgcg cgtcgccacc ggtgtcgcga
     2881 aagcgacggc ggcggcaagc gtcaccagca gcaagcggtt gacgtactcg ccgagcggat
     2941 gcagcgtcgg agcggcgctc gacagtccgc cgagcgcgcg atagtagtcg agcgcgtgcg
     3001 cgaggctgtc gcagcggaac agcacccagc cgcccacgac cacggcgagc gtgtaggcgt
     3061 gcgcgaccac gcggtgccgc tcgaggtggc gcgcgagtcc ggcgcgctcg acgatcagga
     3121 acaggccatg ccatgcgccc cacagcacga acggccagct cgcgccatgc cacaggccgc
     3181 acagcaggaa cacgatgatc aggttcgcat acgcgcggcg cgtgccggcg cggttgccgc
     3241 cgagcggcac gtagacgtag tcacggaacc agttcgacag cgaaatgtgc cagcgccgcc
     3301 agaactcgcg gatgctgccc gcgatgtacg ggtagttgaa gttctcgaga atgcgaaagc
     3361 cgaacatgcg catgagcccg atggccatgt ccgagtagcc ggagaagtcg aagtagatct
     3421 gcagcgtgta gcacgcgatg ccgagccacg cgagcggtgc tgcgagctcg gagcccggca
     3481 gcgcgaagat cgcgtcggcc gtcgcgccga gcgtattggc gatcagcagc ttcttcccga
     3541 gcccgagcac gaagcggcgc acgccgtacg cgaattcgga cacgttcgcg tcgcgcgcga
     3601 caagctgcgc ggcgatgtcg cggtaacgga tgatcgggcc ggcgatcagc tgcggaaaga
     3661 gcaggatgta gagcgcgaat tcgcggatgc tgcgctgcgc gcccgcggtg cgcttgtaaa
     3721 cgtcgaccac gtacgagatc gcgtggaacg tgaagaagga aatgccgagc ggcagcggta
     3781 tcgcgcgcag cgcaaccggc gacagtccga agatcggcgc gattgcgtcg acgttggcga
     3841 ccgcgaagtt ggcatacttg aagaccgcga gcgcggcgag atttgccgcc acggcggccg
     3901 cgagtcgccg cttgcgcgcc ggcggctcgc tcgccgccgc aatgcgcagt ccgatccacc
     3961 agttgaacgc gaccgatcca agaacgagcg gaacgtagcg agcttcgccc caggtgtaga
     4021 acgcgacgct tgcgacgagc aggaccgcgt tgcgaaacct gcgcggaagg gcgaaatacg
     4081 cgacgatcgt cgccggcagg aacaggaaca ggaacagcgg cgacgcgaag accaattact
     4141 tcggataacc ctgacccggt gccggccgtg caggttcgcc ggctgcggcc ggcgccgcgg
     4201 catcgtccgg agccgtgcga ggtgccgcag cagcccctgc cgtcgccggc gcgcgacgtg
     4261 cggcgggtgc cgtgcgcggg tacccggcat cgtcgagcct cgcctgccac agcgtctcgt
     4321 tgtagccctg cagcacctgc ttgtcgccga tcgcgagcac cggcgcggtc tgcttgccgg
     4381 tcagcgcctt caatcgcgcg gcgccctttt cctcgctgac gaccaccgtc tcgaacggaa
     4441 tgccgcgctt gaccagcagt gcctgcgcat cgcggcagat atcgcagtcg aacgtgtaga
     4501 gcgtgaccgg aaagtgctcg gcggcctcgc gcgcctgcaa cggaacggga tcggtttcga
     4561 tcacgttctg cggcagctgc ttcatctgcg cgtccttcgc cgtggccggc ggcggctggt
     4621 cggagtacac gacgcgcccg ttcgggtcga cgtaccggta cagcggttgc gcaagcgcac
     4681 cgctcgcgag ggcggcgatg acaacgacgg acagcgtggc gatcgggctt cgagccatgg
     4741 tgggacgggg agcgctcagg acgcgaccat tccattatgg cgcagaagcg cgtcgagcga
     4801 aggggggcgg ccgcggaagg cgacgaacga ctcgagcgcc gtgcggctgc cgccacgcgc
     4861 gagcacttcg tcccgaaagc gcgcgccgac ctgcggcgac agaacgccgg cctcctcgaa
     4921 caggctgaat gcgtcggccg acagcacctc ggcccattta tagctgtagt agcccgccgc
     4981 gtacccgccc gcgaacacgt gcccgaacgc gtgcatgacg cggtcgtacg gtggtttcgg
     5041 gacgaccgca acttcgcggc gcaccgcgtc gagtaccgcc tcggcactgg ggtagccgcc
     5101 cccggcaccc ggcacatacg cggtgtgaag cagcatgtcg aacatcgcga gctccacctg
     5161 acgcagcatc gccatgccgc tgccgtagtt gcgcgccgcg agcatgcgcc cgaagagctc
     5221 gcggggcagc ggcgcgccgg tcttcgcatg tgccgtcatg tgcgaaagca cgtcccactg
     5281 ccagcaatag ttctccatga actggctcgg cagctcgacc gcgtcccatt cgacgccctg
     5341 caatcccgaa acgcc
//
LOCUS       DASSVX010000209         9681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1358638, whole genome shotgun sequence.
ACCESSION   DASSVX010000209 DASSVX010000000
VERSION     DASSVX010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358638"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFO53_08945"
     CDS             <1..372
                     /locus_tag="VFO53_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8876440.1"
                     /translation="ACFAPLAMSARAGVRSVSQEQIHAAYSMGATKQQVIWHVVVPGA
                     MPEILTAMRIAIGFGWTTLVAAEMVAATVGLGQMVLNASNFLRTDVVIMGIIVIGVVA
                     YLFDLLMRYVEHLVVPWKGKL"
     gene            401..2200
                     /locus_tag="VFO53_08950"
     CDS             401..2200
                     /locus_tag="VFO53_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET8876441.1"
                     /translation="MPPIALKLFPFLAWRNRVDRETLRADLLAGLVGAVMVLPQGVAY
                     ATLAGMPPEYGLYGSMLPAIVGALWGSSWHLMSGPTNATSLMVFATVSAIAVPFSPSY
                     VAIVLTLNLMVGIIKLGLGVARLGSLVNFISTSVVIGFTAGAGLLIIGAQLRNFFGID
                     VPQQASFIAGLRDFATHLGATNPWVLAVGAFTLVASIVGRKAMPRVPYMLTGIVAGAL
                     FAWGLSLAGLAHVPTIGALPSAVPPLSTPETSLRTWQTLAPIALALTVIGLSEAISSA
                     RAVALRSGQRIDGNQEFIGQGLANIVGAFTSSYPTSGSFNRTGANYEAGARTPLACVF
                     SAVLLLVILLLVRPLAGYLPVASMAAVLFIVAMGLIDVQAMRRVWRTSRADALTLAIT
                     FVATLTIRLEVAILVGVLASLLVYLNWATHPLVLRVAPDPSQQRRFRRTGADATLCPQ
                     LDMLRIDGALFFGSVEHIRDEIEAARAQRSAARHVLLIGTGVNLIDTAGAELLANLAT
                     QLREAGVQLYLCKMRPEVLTLLERGGYLDTIGRDRVFATKDKALAAIYGRLDAAKCAA
                     CNARIFNECQVTLPDGSLRDQPRPELTLMPRGG"
     gene            complement(2243..3310)
                     /locus_tag="VFO53_08955"
     CDS             complement(2243..3310)
                     /locus_tag="VFO53_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET8876442.1"
                     /translation="MKKKRSTIYDLADLAGVSASTVSAVLSGNWQARRIGAATAKKVQ
                     RLAAANRYTVNRQASGLRKSRSGLIGMIIPMHDNRFFSSLSQVFERLARERHWYPLVV
                     STLRDPALELETVRTLISYRIEYLVVAGATDPDSVSRICKGHDVAHVNVDLPGTKATS
                     VISDNYWGAQQLTLALIDRSTPQRARNRNKAYFVGGIATDHATRRRMEGFADVIRTRF
                     GRVDPEQLHACGYEGERAEAAVAALHRRLGGLPRALFINSTIALEGVVRYLKTLPAEE
                     LRRCAFGCYDWDPFASVLSFPLLMVRQNVEGLLGEAFRIIDARSFTGNRIIEIKPDLL
                     FAQPSGGASASSRSIACVVAR"
     gene            3521..5017
                     /locus_tag="VFO53_08960"
     CDS             3521..5017
                     /locus_tag="VFO53_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET8876443.1"
                     /translation="MASRQPERPNILFILSDDQGAWALGCAGNPEIRTPNLDRLAQRG
                     VRFDNFFCTSPVCSPARASLLTGDIPSRHGVHDWIRVGSVGANRVDYLAGQTLLTDVL
                     AGEGYRCGLVGKWHLGASDVARPNFVKWFAHQSGMGPYYNAPMIDHDRPVIVEGYLSD
                     VLSDVARAFVHDEATRAEPFWLSLCFTAPHYPWIDSHPKPFTDLYADCAFTSCPDEAP
                     HPWFTGGKPAIAKGRRERRESLIGYFAAVSAMDAAIGSVLDELDAQGLAESTLVVFMS
                     DNGMNLGHHGIWGKGNGTRPQNMYDTSVKVPCILSQPGRIPAGRVSDALVSGYDVFPT
                     ILDYVDARWASPRPRPGRSFRRLLEGQPASPEMPVVVYDEYGPVRMVRTSGWKYVHRY
                     PDGPHELFDLRADPGERSNLIDDTEAAPTLASLRERLDAWFRTYVDPRRDGSDRGVTG
                     CGQLGLVENATPDHPVFADSHLVGADWDLWVAGGNGKAAQDIGLDNAE"
     gene            5026..6111
                     /locus_tag="VFO53_08965"
     CDS             5026..6111
                     /locus_tag="VFO53_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET8876444.1"
                     /translation="MSIADDARMTAIVCHAPEDYRVETIARPRAGPREMVIRIGACGI
                     CASDCKCWSGAKMFWGGNGTAPWVKAPVIPGHEFFGYVEALGDGAAEHFGVKLGDRVI
                     AEQIVPCERCRYCRSGKYWMCEVHNIFGFQRDVADGGMAQYMRIPPTAIVHAIPPELP
                     PDDAAIIEPLACAIHAVNRGDIQLDDVVVIAGAGPIGLLMVQVAKLKTPRKLIVVDMV
                     AERLALARRYGADVTINPNDDDALAIVHGLTGGYGCDVYIEATGSPSGAVQGLELIRK
                     LGRFVEFSVFGKDTTADWSIIGDRKELDVRGAHLGPYCYPIAIDLLARGLVTSKGIVT
                     HAYALEQWNEAIGVANSLDSIKVLMKP"
     gene            6161..7171
                     /locus_tag="VFO53_08970"
     CDS             6161..7171
                     /locus_tag="VFO53_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876445.1"
                     /translation="MNRTRRTFVAVAASLAMLAAAQQGALAADANAPGKGITINIGYF
                     PVVSPVPIMRTQHLLEDKGYTVNWVPISQGLPGAASAIAAGKLDIAWGNSISAVVIFS
                     QSPDAAQFIGQSFINANVTVVAEKSPIKSAKDIVGKKVVVSGMKTASTLFFQIGLKKA
                     GVDPNANEYFVSGTGPGMVGVLDSGGAEVVAGYVPYVADMELKKIGRVLFTGSDAIGR
                     PAPGDGFIASTRFIHDHPDAVKDVLRAQFAATDFIKTHPDDAYKSMAEFAKVDEAAVR
                     YSFEKKLIGVAASYVPDVEGIVETEKVAQELGFAPKGVDLPEFTKKFENTALAKEVLA
                     KR"
     gene            7200..8027
                     /locus_tag="VFO53_08975"
     CDS             7200..8027
                     /locus_tag="VFO53_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876446.1"
                     /translation="MATAPAIIATTSPRRLSPRALAWVLGAAGGVVAIAAWTLLSHLL
                     AHGGGVINRLPGPLSVVRELVQYAGAGLAKDLAFSLRVFAIGWLIGAATAALTGLLLG
                     RARWLGNVFLPVVEAIRPVSSIAWVPLSIVWFGFGLTSKVFLVGLAVFLVVIVYAVDG
                     SRRVPADLERTATMLGMNGWQRFRSLVLPGTLAEVLIGSRVALMAGWGTVIVAELVAA
                     DSGLGAHLISVEQSYDVPAVIATMICFGVTGLLMNGAFSFAERRLMPWRHDGETLDR"
     gene            8024..8788
                     /locus_tag="VFO53_08980"
     CDS             8024..8788
                     /locus_tag="VFO53_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876447.1"
                     /translation="MSPGAHLLTRRVLLPLVGVAGVLAAWEATLFVYRLPAIVLPSPW
                     SVATTLGELAGTGAFWRDVGVSLYEFIAGYVIGVVLGVAVGMLIGEWRHFRMAATPVI
                     ELLRFIVPFAWIPLTILWFGTSYTGKILLVAYAVFFVMVVSTARAVTHVEPTLSRVGT
                     MLGMGPWRLAWTVHLRAAAPLLASAARAAAAIGWIAVVAAEYIGASAGLGLMITNAAT
                     SLATPVVIAGMIVIGVVGAGVSAAIGALSRAWLDYR"
     gene            8770..>9681
                     /locus_tag="VFO53_08985"
     CDS             8770..>9681
                     /locus_tag="VFO53_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8876448.1"
                     /translation="MARLPMSAAGPSQGAHSAPSGGSAAAKPQAWGDHTSDAQTLASG
                     AGVRTGELVVDRVFHRYVVGGRPTEVLRDVSFRVAPGTVACVVGPSGCGKSTLLGLAA
                     GLLRPTSGTVSWDGAPVRIGRNPHLGMAFQQPGLFPWMSVRQNVAIGLRTRGHPRRDA
                     RRIADEFLETVGLAEFKDAYPSHLSGGMAQRVGIARALALRPRLLLLDEPFAAVDAFT
                     RLKLQQEFKTLLKLNRPTVLFVTHDVPEAIALGDVIVVMSQRPAAVQRIIPVAREERD
                     RASPAYARKLAETLECLGVTSDIGDQET"
BASE COUNT         1436 a         3335 c         3259 g         1651 t
ORIGIN      
        1 gcgtgcttcg cgccgcttgc gatgagcgcg cgcgccggcg tccgttcggt gtcgcaggag
       61 cagatccatg ccgcgtactc gatgggggca accaagcagc aggtgatctg gcacgtggtc
      121 gtgcccggtg cgatgccgga aatcctgacg gcgatgcgca tcgcgatcgg cttcggctgg
      181 acgacgctgg tcgccgccga aatggttgcg gcaacggtcg gcctcggcca gatggtgctg
      241 aacgcgtcga acttcctgcg caccgacgtc gtgatcatgg gcatcatcgt gatcggcgtc
      301 gtcgcgtatc tgttcgacct cctgatgcgc tacgtcgagc atctcgtggt tccgtggaag
      361 ggcaagctct gactcccggt tgccgggtcc tgcgcgcatc gtgcctccga tcgcgctgaa
      421 gctcttcccg tttctcgcct ggcgcaatcg cgtcgatcgc gagacgctgc gcgccgatct
      481 gcttgcaggc ctcgtcggtg cagtgatggt gctgccacag ggcgtcgcgt acgcaacgct
      541 cgccgggatg ccgcccgaat acggcctcta cggctcgatg ctcccggcga tcgtcggcgc
      601 gctgtggggc tcgtcgtggc acctgatgtc gggcccgacg aacgcgacgt cgctgatggt
      661 gttcgccacg gtcagcgcca tcgccgtgcc gttctcgccg agctacgtcg cgatcgtgct
      721 cacgctgaat ttgatggtcg gcatcatcaa gctcggcctc ggcgtcgcac ggttgggttc
      781 cctcgtcaac ttcatctcga cgtcggtcgt catcggcttc accgccggcg ccggcctgtt
      841 gatcatcggc gcgcaactgc gcaacttctt cggcatcgac gtcccgcagc aggcgagctt
      901 catcgccggg ctgcgcgatt tcgcgacgca cctcggcgcg acgaatccgt gggtgcttgc
      961 cgtcggcgca ttcacgctcg tcgcgtcgat cgttggccgc aaagccatgc cgcgcgtgcc
     1021 ctacatgctg accggcatcg tcgccggcgc gctcttcgca tggggacttt cactcgccgg
     1081 ccttgcgcac gtgccgacga tcggcgcgtt gccttccgcg gtgccgccgt tgtcgacgcc
     1141 agaaacgtcg ctgcgcacgt ggcagacgct tgcgccgatc gcgctcgcgc tgaccgtcat
     1201 aggactgtcg gaagcgatct cgagcgcacg cgccgttgcg ctgcggtcgg gccagcgcat
     1261 cgacggcaac caggaattca tcggccaggg cctcgcgaac atcgtcggcg cgttcacgtc
     1321 gagctatccg acgtccggct cgttcaaccg caccggcgcc aactacgagg ccggcgctcg
     1381 cacgccgctc gcatgcgtgt tctcggcggt cctgctgctc gtgatcctgc tgctcgtgcg
     1441 gccgctcgcc ggctatctgc cggtcgcgtc gatggccgcc gtgctgttca tcgtcgcgat
     1501 gggcctgatc gacgtgcagg cgatgcgccg cgtgtggcgc acgagccgcg ccgacgcgct
     1561 cacgctcgcc atcacgttcg tcgcgacgct cacgatccgg ctcgaggtcg cgatcctcgt
     1621 cggcgtgctt gcgtcgctgc tcgtgtacct caactgggcg acgcatccgc tggtgctgcg
     1681 cgtcgcaccc gacccgtcgc agcagcggcg cttccggcgc accggtgccg acgcgacgct
     1741 gtgtccgcag ctcgacatgc tgcgcatcga cggcgcgctg ttcttcggct cggtggagca
     1801 catccgcgac gagatcgagg ccgcgcgcgc gcaacgctcc gccgcgcgcc acgtgctgct
     1861 gatcggcacc ggcgtcaacc tgatcgacac cgccggcgcc gagcttctcg ccaacctcgc
     1921 gacacagctg cgcgaagcgg gcgtgcagct gtacctgtgc aagatgcgcc ccgaggtgct
     1981 gacgctgctc gagcgcggcg gctaccttga caccatcggc cgcgatcgcg tgttcgcgac
     2041 caaggacaag gcgctggcgg cgatctacgg caggctcgac gcggcgaaat gcgccgcatg
     2101 caacgcgcgc atcttcaacg agtgccaggt gacgctgccc gacgggagcc ttcgcgacca
     2161 gccacggccg gagttgacgc tgatgccgcg tggcggctga gcgcgcgata gccattcccg
     2221 ggcggcgccc gctcgccgca cgtcaacgtg caacgacgca agcgatggag cgcgacgatg
     2281 cgctcgcgcc gccggacggc tgcgcgaaca gcagatccgg cttgatctcg atgatccggt
     2341 tcccggtgaa cgagcgcgcg tcgatgatcc ggaacgcttc gccgagcagg ccctcgacgt
     2401 tctggcgcac catcagcagc ggaaagctca gcacgcttgc gaacgggtcc cagtcgtagc
     2461 agccgaacgc gcagcggcga agctcctcgg cgggcagcgt cttgaggtag cggacgacgc
     2521 cttcgagcgc gatcgtcgag ttgatgaaaa gcgcgcgtgg aagtccgccg agccgccggt
     2581 gcagcgccgc cacggcggcc tcggcgcgct cgccctcgta gccgcacgcg tgcagctgtt
     2641 cgggatcgac gcgcccgaac cgcgtgcgga tcacgtcggc gaagccctcc atccttcgtc
     2701 gtgtcgcgtg gtcggtcgcg atgccgccga cgaagtacgc cttgttccga ttgcgcgccc
     2761 gctgcggcgt cgagcggtcg atcagcgcca gcgtcaactg ctgcgcaccc cagtagttgt
     2821 cgctgatcac cgaggtcgcc ttcgtgccgg gcaggtcgac attgacgtgt gccacgtcgt
     2881 gacccttgca gatgcggctc accgagtcgg gatcggttgc gccggcaacg accaggtatt
     2941 cgatgcggta cgaaatcagc gtgcgcaccg tctcgagctc gagcgccgga tcgcgcagcg
     3001 tgctgacgac cagcgggtac cagtggcgct cgcgcgccag ccgctcgaac acctgcgaca
     3061 ggctgctgaa gaagcggttg tcgtgcatcg ggatgatcat tccgatcagc cccgagcggc
     3121 tcttgcgcag cccgcttgcc tggcggttga ccgtatagcg gttggcggcg gcaagccgct
     3181 gcaccttctt cgccgtggcg gcaccgatac gccgcgcctg ccagttgccg ctcagcacgg
     3241 cactgacggt cgatgcagac acgcccgcga gatccgcgag atcgtagatc gtcgatcttt
     3301 tctttttcat cattgtcgcg gcggcggaca gggtggctgg atcggcggca tcggggcgcc
     3361 gatgccgccg ttgcgcacgg ccgtgccggc tggcacaatc gattgtgcag cataagcgcg
     3421 cgcggctgcc gccgtcaaac atggcacaat ccgcacaatc gattgtgcag cacgggcgcg
     3481 agccgtgccc cggccgggaa gcaatgcgag gagagcgagc gtggcaagca ggcagccgga
     3541 acgtccgaac atcctgttca tcctgagcga cgaccagggc gcgtgggcgc tcggctgcgc
     3601 cggcaatccc gaaatccgca cgccgaacct cgaccgcctc gcgcagcgcg gcgtgcgctt
     3661 cgacaacttc ttctgcacgt cgcccgtctg ttcgccggcg cgcgcctcgc ttctgaccgg
     3721 cgacatcccg tcgcgccacg gcgtgcacga ctggatccgc gtcggcagcg tcggcgccaa
     3781 ccgcgtcgac tatctcgccg ggcagacgct tctcaccgac gtgctcgcgg gcgagggtta
     3841 tcgctgcggg ctcgtcggca aatggcatct cggcgcaagc gacgtggcgc gtcccaattt
     3901 cgtcaagtgg ttcgcgcatc agtcgggcat gggtccctac tacaacgcgc cgatgatcga
     3961 ccacgatcgt ccggtgatcg tcgagggcta cctgtccgac gtgctgtccg acgttgcccg
     4021 cgcgttcgtg cacgacgagg cgacgcgtgc cgaaccgttc tggctttcgt tgtgcttcac
     4081 cgccccgcac tatccgtgga tcgacagcca tccgaagccg ttcaccgatc tctacgcgga
     4141 ctgcgcgttc acctcgtgtc ccgacgaagc gccgcatccc tggttcaccg ggggcaagcc
     4201 ggccatcgcg aagggccgcc gcgagcgccg cgaaagcctg atcggctact tcgccgcggt
     4261 cagcgcgatg gatgccgcga tcggcagcgt gctcgacgag ctcgatgcgc agggcctggc
     4321 cgaaagcacg ctcgtcgtgt tcatgagcga caacgggatg aacctcggcc accacggcat
     4381 ctggggcaag ggcaacggca cgcgcccgca gaacatgtac gacacgtccg tgaaggtgcc
     4441 gtgcatcctg tcgcaacccg gccgcattcc cgcgggacgc gtcagcgacg cgctcgtgag
     4501 cgggtacgac gtctttccga cgatcctcga ttacgtcgat gcgcgctggg catcgccgcg
     4561 gccgcggccc ggccgcagct tccgccgcct cctcgaggga cagccggcga gcccggagat
     4621 gccggtggtc gtctacgacg aatacggccc ggtgcgcatg gtgcgcacct ccggctggaa
     4681 gtacgtgcac cgctatcccg atggcccgca cgagctcttc gacctgcgcg ccgatccggg
     4741 cgagcgctcg aacctgatcg acgacaccga ggccgcgcca acgcttgcgt cgctgcggga
     4801 gcgcctcgac gcgtggtttc gtacctacgt cgacccgcgg cgcgacggca gcgacagagg
     4861 cgtcaccggc tgcggccagc tcggactcgt cgagaacgcg acgccggacc atccggtgtt
     4921 cgccgattcg catctcgtcg gcgccgactg ggacctctgg gtcgcgggcg gcaacggcaa
     4981 ggccgcccag gacatcggcc tcgacaacgc ggagtgacga aagacatgag catcgccgac
     5041 gacgcgcgca tgaccgccat cgtgtgccac gcacccgagg attaccgggt cgagaccatc
     5101 gcacgtccgc gtgccggtcc gcgcgaaatg gtgatccgca tcggcgcctg cggcatctgc
     5161 gcaagcgact gcaaatgctg gtcgggtgcg aagatgttct ggggcggcaa cggcaccgca
     5221 ccctgggtca aggcgccggt gatccccggt cacgaattct tcggctacgt cgaggcgctc
     5281 ggcgacggcg cggccgagca cttcggcgtg aagctcggcg atcgcgtcat cgccgagcag
     5341 atcgtgccgt gcgagcgctg ccgttattgc cgctccggca agtactggat gtgcgaggtg
     5401 cataacatct tcggcttcca gcgcgacgtg gccgacgggg gcatggcgca gtacatgcga
     5461 attccgccca cggcgatcgt gcacgcgatt ccgcccgagc tgccgcccga cgacgcggcg
     5521 atcatcgagc cgctcgcctg cgccatccac gcggtcaatc gcggcgacat ccagctcgac
     5581 gacgtcgtcg tcatcgccgg cgcgggcccg atcggtctcc tgatggtgca ggtggcgaag
     5641 ctcaagacgc cgcgcaagct gatcgtcgtc gacatggtcg ccgagcgcct cgcgctcgca
     5701 cgccgctacg gcgccgacgt gacgatcaat ccgaacgacg acgacgcgct cgccatcgtg
     5761 cacggactca ccggcggcta tggctgcgat gtctacatcg aagccaccgg cagccccagc
     5821 ggcgccgtgc aggggctcga gctcatccgc aagctcggcc gcttcgtcga attctccgtg
     5881 ttcggcaagg acaccaccgc cgactggtcg atcatcggcg accgcaagga gctcgatgtt
     5941 cgcggcgcgc atcttggtcc gtattgctac cccattgcca tcgacctgct cgcgcgcggc
     6001 ctcgtgacgt cgaagggcat cgtcacgcac gcctacgcgc tcgagcaatg gaacgaggcg
     6061 atcggcgtcg cgaattcgct cgactcgatc aaggtgctga tgaagccctg agctcgctcg
     6121 aaaccccgta tcggtccgga acaatcacaa ggaggagacc atgaatcgca ctcgtcgcac
     6181 gttcgtcgcc gtcgccgctt cgctcgccat gcttgccgcc gcgcagcaag gcgcgctcgc
     6241 ggctgacgcc aatgcccccg gcaagggcat cacgatcaac atcggctatt ttccggtcgt
     6301 ctcgccggtg ccgatcatgc gcacgcagca cctgctcgag gacaagggct acacggtcaa
     6361 ctgggtgccg atctcgcagg gcctgccggg tgcggcgagc gcgatcgccg cgggcaagct
     6421 cgacatcgca tggggcaaca gcatctcggc ggtggtcatc ttctcgcagt cgcccgacgc
     6481 cgcgcagttc atcggccagt cgttcatcaa cgcgaacgtc accgtggtgg cggagaaaag
     6541 cccgatcaag agcgccaagg acatcgtcgg gaagaaggtc gtcgtgagcg ggatgaagac
     6601 cgcgtcgacg ctcttcttcc agatcggtct caagaaggcg ggcgtcgatc cgaacgcgaa
     6661 cgagtatttc gtctcgggca ccggtcccgg catggtgggc gtgctcgatt cgggcggcgc
     6721 cgaagtcgtc gccggctacg ttccgtacgt tgccgacatg gaattgaaga agatcggccg
     6781 tgttctgttc accggctcgg acgccatcgg ccggccggcg cccggcgatg gcttcatcgc
     6841 gagcaccagg ttcatccacg atcatcccga tgcggtgaag gacgtgctgc gcgcgcagtt
     6901 cgcggcaacc gacttcatca agacgcatcc cgacgacgcg tacaagtcga tggccgagtt
     6961 cgcgaaggtc gacgaagccg ccgttcgtta ttcgttcgag aagaagctga tcggcgtggc
     7021 cgcgtcgtac gttcccgacg tcgagggcat cgtcgagacg gaaaaggtcg cgcaggagct
     7081 cggcttcgcg ccgaagggcg tcgatctgcc cgagttcacg aagaagttcg agaatacggc
     7141 gctcgcgaag gaagtgctgg cgaaacgcta ggctcggccg cacggacctc ggcgaacgca
     7201 tggcgaccgc gcctgcgatc atcgcgacga catcgccgcg gcggctttcg ccgcgtgcgc
     7261 tggcgtgggt gctgggcgcg gccggcggcg tcgtcgcgat cgccgcatgg acgctcctgt
     7321 cgcatctgct cgcgcacggc ggcggcgtca tcaatcgcct gccgggaccg ctttccgtcg
     7381 tgcgcgagct cgtgcagtac gccggcgcgg gtctcgccaa ggatctcgcg ttcagcctgc
     7441 gcgtgttcgc gatcggctgg ctgatcggtg cggcgactgc cgcgctgacc ggtctgctgc
     7501 tcggccgcgc gcgatggctc ggcaacgtct tcctgcccgt cgtcgaggcg atccgtccgg
     7561 tgtcgagcat cgcgtgggtg ccgctttcga tcgtgtggtt cggcttcggc ctcacgagca
     7621 aggtgttcct cgtcggcctc gccgtattcc tcgtcgtgat cgtctacgcg gtcgacggat
     7681 cgcgtcgcgt gccagccgac ctcgagcgaa cggcgacgat gctcggaatg aacggatggc
     7741 agcgctttcg cagcctcgtg ctgccgggca cgcttgccga agtgctgatc ggctcgcgcg
     7801 ttgcgctgat ggcgggatgg ggcacggtga tcgttgccga gctggtcgcc gccgactcgg
     7861 gactcggcgc acacctcatt tcggtcgagc agagctacga cgtgccggcc gtcatcgcga
     7921 cgatgatctg cttcggcgtc accggcttgc tgatgaacgg ggcattctcg ttcgccgagc
     7981 gccgcctgat gccgtggcgc cacgacggcg aaacgctcga ccgatgagcc caggcgctca
     8041 tctgctcacc cgccgcgtct tgctgccgct cgtcggcgtc gcgggcgtgc tcgccgcgtg
     8101 ggaggcaacg cttttcgtct atcgcctgcc cgcgatcgtg ctgccgtcgc cgtggtcggt
     8161 cgcgacgacg ctcggcgagc tcgccggcac gggcgcgttc tggcgcgacg tcggcgtcag
     8221 cctgtacgag ttcatcgccg gctacgtgat cggcgtcgtg ctcggggtcg ccgtcggcat
     8281 gctgatcggc gaatggcgcc acttccgcat ggccgcgaca ccggtcatcg agctgcttcg
     8341 cttcatcgtg ccgttcgcat ggatcccgct gacgatcctc tggttcggga cgagctacac
     8401 gggcaagatc ctgctcgtcg cgtacgcggt gtttttcgtg atggtcgtgt cgacggcgcg
     8461 cgccgtgacg cacgtcgagc cgacgctgtc gcgcgtcggc acgatgctcg gcatgggccc
     8521 ctggcgtctt gcctggacag tgcacctgcg cgcggcggcg ccgctgctcg ccagcgcggc
     8581 gcgcgcggcc gccgccatcg gctggatcgc cgtcgttgcc gccgaataca tcggcgcgag
     8641 cgcgggcctc ggattgatga tcacgaatgc ggcgacgtcg ctcgcgacgc cggtcgtgat
     8701 cgccggcatg atcgtgatcg gcgtcgtcgg tgcgggcgtc agcgccgcca tcggcgcgct
     8761 ctcgcgcgca tggctcgatt accgatgagc gccgccgggc cgtcccaagg cgctcattcc
     8821 gccccctcgg ggggcagcgc agcggcgaag ccgcaagcgt ggggggatca tacaagcgat
     8881 gcgcagacgc tcgcatccgg cgccggcgtg cgcaccggcg agctcgtcgt cgatcgcgtg
     8941 tttcatcgct acgtcgtcgg cggccggccc accgaggtgc tgcgcgatgt ttcgtttcgc
     9001 gtggcgcccg gcaccgttgc ctgcgtcgtc gggccgtcgg gatgcggcaa gagcacgctg
     9061 ctcggtcttg ccgccgggct cttgcgtccg acgagcggca cggtgagctg ggacggtgcg
     9121 ccggtgcgca tcggccgcaa tccgcatctc ggcatggcgt tccagcagcc gggcctcttc
     9181 ccgtggatgt ccgtgcgcca gaacgtcgcg atcggattgc gcacgcgcgg acacccccgc
     9241 cgcgatgcgc gacgcatcgc cgacgaattc ctcgagaccg tgggcctggc cgagttcaag
     9301 gacgcctatc cgagccacct gtccggcggc atggcgcagc gcgtgggcat cgcgcgcgca
     9361 ctcgcgcttc gaccgcgcct gctcctgctc gacgagccgt tcgccgcggt cgatgcgttc
     9421 acgcggctca agctgcagca ggagttcaag accctgctga agctcaaccg gccgacggtg
     9481 ctgttcgtga cgcacgacgt gcccgaagcg atcgccctcg gcgacgtgat cgtcgtcatg
     9541 tcgcagcgtc cggccgccgt gcagcggatc attccggtgg cccgcgaaga gcgcgaccgc
     9601 gcgagccccg cctatgcgcg aaagctcgcc gagacgctcg aatgccttgg cgtgacgagc
     9661 gacatcggcg accaggaaac g
//
LOCUS       DASSVX010000210        18365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_658626, whole genome shotgun sequence.
ACCESSION   DASSVX010000210 DASSVX010000000
VERSION     DASSVX010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 18365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18365
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658626"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VFO53_08990"
     CDS             complement(<1..1185)
                     /locus_tag="VFO53_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017297227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET8876449.1"
                     /translation="MNESSEAGGDFREHLIRYGGDAYPEIVEKAQGSWVVDATGRRIL
                     DFTSGQMCATVGHNHPNIVAAIKRSCDNALHLFSGMIPRSVVQLADALAKILPAPLKK
                     SLFLNTGSESNEAAIKMAKLYTGGFEVVGLGGSWHGVTGNAGGVSFASDRKGYGPGMP
                     GTYVIPEPNAYRCPVRHCRDKCDRTCMKVGFELYDMQSSGARAAVIAEPVISAGGVIV
                     PPEGYFDALQQEACKRNMLLIFDEAQTAFGRLGHWFAASQLGVTPDLMTVSKTLGGGV
                     PLAAVITNDEIEATCHRRGFAFYTSHVSDPLPSTVGLAVLETVAQENLLARSREMGDY
                     LAARLTELEARRRAEQVRRDELKQAISASGGDRLQRLRADVAAKTVDKEARAAKADQY
                     DSF"
     gene            1283..1852
                     /locus_tag="VFO53_08995"
     CDS             1283..1852
                     /locus_tag="VFO53_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876450.1"
                     /translation="MAGSDTRLLEEWKANLDLLKFYETLKQQRFAHFLTIQTAFLAVF
                     GLVAKDAATVFSLVTIATLAVVPIPPLFISFYFMRLDRRGRAFVDTANTRLLLIEREW
                     QQRFPDDHFATYQQQYGVLSRRDQTLIAQYVRTRNVAGDPYPMLVNAPSAHASETAII
                     RLFWWLWVILFSGFALHLAWHLANPGWRP"
     gene            complement(1849..2343)
                     /locus_tag="VFO53_09000"
     CDS             complement(1849..2343)
                     /locus_tag="VFO53_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HET8876451.1"
                     /translation="MDLAAFLAHHGIDAKRFEHSPVMTVEESKRLVPDLPGAKTKNLF
                     LRDKKGARHILLTVAHDVAVDLNSLGLLLGTGRLGFASPERLRKYLGVAPGAVSLLAL
                     ANDTAHAVEFVLDRRLWDADAVQAHPLVNDATMVLPHTQLERFLAATGHAAQIVDVPG
                     RRES"
     gene            complement(2455..2694)
                     /locus_tag="VFO53_09005"
     CDS             complement(2455..2694)
                     /locus_tag="VFO53_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876452.1"
                     /translation="MKRIPLGSLLAITLCALLSACASTTTRVTTDSDAYKTMARSQQW
                     WCSQFGCGCSIDGQQATCALVSTCINTGSCKTATQ"
     gene            complement(2917..4143)
                     /locus_tag="VFO53_09010"
     CDS             complement(2917..4143)
                     /locus_tag="VFO53_09010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET8876453.1"
                     /translation="MSAAAKPQAWGDRASAAAVPRRLRRVLSLDDFEAAGRRHLPRPV
                     FGYVAGAVETDWSLADNRAAFAEFGFLPRMLVDVSQRSAQTTLLGHAYSAPFGIAPLG
                     ISALYAYRGDLVLASAAERANVPMIMSGSSLIRLEEVVSACPSAWFQAYLPGDVPAID
                     ALVERVGQAGFRTLVVTVDSQVAGNRENNIRTGFSTPLRPTLRLAWDGLTHPRWLFGT
                     FLRTLGRHGMPHFENNFATRGAPILSASVLRDYSDRGNLHWEHWRRVRRMWNGRLVIK
                     GVLDKADATLARDAGADGIIVSNHGGRQLDGAVAALRVLPGIVAACPDVPVMLDGGVR
                     RGTDVLKALALGAKFVFAGRPFGYAAAIGGEAGVAHAIALLSAEIERDMAMLGINELA
                     ELGPERLLRVGGRALD"
     gene            4332..4793
                     /locus_tag="VFO53_09015"
     CDS             4332..4793
                     /locus_tag="VFO53_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET8876454.1"
                     /translation="MTPAIDTAAVSDLPDTKPRHGILRLVNRIRVELVDALDRELAPF
                     DISAPQLFVLSSVANREADSAAGLCKSISYDPGAMTRMIDRLQQKGLVRRVPHPDDRR
                     AMNLELTVAGKALFPQLVSAKDRVQAQFLRGFSKDDAHALESLLHRMLANR"
     gene            4875..6341
                     /locus_tag="VFO53_09020"
     CDS             4875..6341
                     /locus_tag="VFO53_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET8876455.1"
                     /translation="MTLPITRAALACACACALLAGCASMSGIAPQAQSRNANELASQH
                     ALSAVSTSSAAWPKADWWKAYGDPQLDALIDEALAHSPTLDVAAARTRKALAVADAGR
                     SALSPRIDASASSTREHFSEHGLVPPPFGGTTLTLNQMQLALSWDPDVWGKNRSAYEA
                     ALGSARAAQIDAHAARLQLSTGVAQAYVQLQRAYLQRDVAQATLAAREKVVALTRDRN
                     AAGLDSRLEVKQAEAALPATREEIAALDERIGLARNQIAALLGAGPDRGLAIARPAAT
                     ALAAIALPSRLPAELLGRRPDIVAQRWRIEAASKNVESQKAEFYPDVNLLAFVGLQSL
                     GGASLLTAASRMLGAGPAVTLPVFDAGRLRAQLAAKDADHDVAVDQYNQTLADAMREV
                     VDQIVSFRSLDEQRRQQRDAAATTREAYDLALIRYREGIGNYLQVLSAEQPMLAQQSL
                     DADLSARALELSINLVRALGGGYEPEPAQVSMTTGNPR"
     gene            6338..7597
                     /locus_tag="VFO53_09025"
     CDS             6338..7597
                     /locus_tag="VFO53_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET8876456.1"
                     /translation="MSDAKPTSRSAANGARKRWLAIVAGAFALIGIAYGAYWALVLRY
                     EQSTDDAYVSGNVVQITPQIAGTVVRIAADDTQFVKAGSTLVELDPADAKIALEQADA
                     KLAKTVRDVRGLFATTAQLKANVDMRNAELARASEDLNRRERLARSGAVSGEELQHAR
                     DALTGAKASLSAAQQELAANQARVDRTSVENHPDVLAAAAQVHDAYLDVARTALPAPV
                     SGYVAKRAVQLGQRVAPGAPLMAIVPLDQVWVDANFKEPQLATMRIGQPATLTADLYG
                     GKVRYHGTVVGFGAGTGSAFALLPAQNATGNWIKIVQRVPVRVALDAQELTAHPLQIG
                     LSMQVEVDTHQRGGDRLPQIARATTGHSTDMYDSVDAAAAKRVHAIIAANERDSASER
                     AASVATAHVARAGAAASAARQLAVSRP"
     gene            7616..9226
                     /locus_tag="VFO53_09030"
     CDS             7616..9226
                     /locus_tag="VFO53_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET8876457.1"
                     /translation="MSRALSTTDPQSRRTPAPAAPASHEPAPLSGATLALGTVALSLA
                     TFMNVLDTSIANVSIPAIAGDLGVSPDQGTWVITSFGVANAISLPLTGWLTQRYGQVR
                     LFVGSVLLFVLASFLCALAPTLGLLIAFRVVQGAVAGPMIPLSQSLLLSSYPKAKAGT
                     ALALWGITTLVAPVVGPVLGGFLTDNIAWPWIFYINIPVGLAAVAVTWMIYRPRETPT
                     AKLPIDAIGLGLLVLWVGALQIMLDKGKDLDWFGSTEITILGIAALVGFALFVVWELT
                     EEHPVVDLRLFGRRNFWTSSLAMSLAYGAFFGNVVLLPLWLQQYMGYTATEAGLVLAP
                     VGLLAILLTPFVGRTVNRVDPRLFATGAFLIFALVLYMRSKFNTSADLWTLMVPTIIQ
                     GAAVAIFFIPLVSLSLSGLPPNRIPSASGLFNFARITAGSFGTSIATTFWDHRATLHH
                     AQIVERLTEANPATTQALAAMHANGLTPEQGYAALNRLVDQQAFMLSANDVFYVSALL
                     FVALIGVVWLARPAKGAAGAGEAAAGAH"
     gene            9231..9512
                     /locus_tag="VFO53_09035"
     CDS             9231..9512
                     /locus_tag="VFO53_09035"
                     /inference="COORDINATES: protein motif:HMM:NF022947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Atu4866 domain-containing protein"
                     /protein_id="HET8876458.1"
                     /translation="MTGAASDVVGVWLSSDGRMRVDLKADGTFDEARAGSARTFHGVW
                     RSDGSRVHFRDPATGYEASGELCDGVLHADGVEFRKAAGISAGAATDAT"
     gene            complement(9481..10212)
                     /locus_tag="VFO53_09040"
     CDS             complement(9481..10212)
                     /locus_tag="VFO53_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876459.1"
                     /translation="MTALVAFLLAWQFLVPLVNIPDYVLPLPSAIASRFAETFMLQVA
                     SLAVTAMTTLAGLAIALVVGVLLALLVVYVEGVKSVIMPFLAAFNSIPKIAIAPLFVI
                     WFGLGVESKILLAFLLALFPIFVNSMTGLGEIEPDILDLARLAGGSRYRVFTMVRLMH
                     ATPYITDALKVAFPLALVGSIVGEFIGGNRGIGQLVLSAQFNLDTALVFAALLSITLF
                     TTAGIGLITLFERRFLKWRPSQHRR"
     gene            complement(10279..11106)
                     /locus_tag="VFO53_09045"
     CDS             complement(10279..11106)
                     /locus_tag="VFO53_09045"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876460.1"
                     /translation="MSVALELVEVRKSFGTGVAVERAAFAVEGNEFVSIVGPSGCGKS
                     TLLRMVAGLVAPSGGRITVAGKAVSGPIENVGMVFQSPVLLPWRTTLANILFVAEVAG
                     RKAPAHRERALELIALAGLGGFENALPHELSGGMQQRAAICRALLMNPPLILMDEPFG
                     ALDVMTRERMAFELQKIWAANRNTVLFVTHSITEAVLLSDTIVVMTPRPGRIQDIVKV
                     DLPRPRDVKVLEHPRFIALSARVRNGIGQGPETTFMGSETTFIEPSSYDEQHAESRL"
     gene            complement(11110..12108)
                     /locus_tag="VFO53_09050"
     CDS             complement(11110..12108)
                     /locus_tag="VFO53_09050"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876461.1"
                     /translation="MKVRMVVSAIVALCAVVCASQAPAQAQAPLKKITFLTNYTFHGR
                     HSPFFVGLDKGYYRDAGFDIEIAPATGSGFVITAVDTGKADYGMAEAGPVVQAVAKGA
                     KVRSFMVFTDISTSGLASLKPYNTLESLKGQRIAASQTDSARVILPILLDRAKIDPAS
                     IQWLAADPGVYSSLLLSGQTDLFTASIDGDIPALEKIAEPRGKKVYFAPFADWGYDVF
                     GYFLIAKDTTLASDPGAAKRFAEATRKAVDYSVAHPEETAKIMVKHNPALNYDTTLAQ
                     WRQTIKSIETPYVKKNGYGVATDERMQRTIDLVKTAMKLDTKLDARDLYTPVLSGH"
     gene            complement(12178..13506)
                     /locus_tag="VFO53_09055"
     CDS             complement(12178..13506)
                     /locus_tag="VFO53_09055"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HET8876462.1"
                     /translation="MREYMEALRARGELLEVTREVHPKHELAAVTSAAHRRWGKPILF
                     HRVGGTALPVLTNIYGSRGRLAEILGIAPDEFCRKWNDLASVAGTRDVALRPALRPTG
                     NLVDCKVSDLPLITYSERDAAPYFTSAMFLAHEPETGVRNLSFHRSMFVSDDELRCRL
                     APRHHLTMYHEKAEKMGRPLEAAMLIGPPPTAFLTAASPLPYDADELEVAAKLAGESI
                     EMRPCRHIDMMVPASTEIVIEGRFLPNVRRDEGPFGEFMGYYTPVGQNAVFEVLGVTR
                     RADAIFHSILCGSSEEVLTLELSVAANIFQRINAVLPGIVDVTCHPFVTNVVVKIRPQ
                     YEGHARQVVLAVFGAEPTWAKMCTVVDEDVDIYDVEDVMWAVLTRSRPDKDTIVIPDT
                     PSFYRDAYKDHWGRMGIDATAPFDRRKDFERKRIPGADSVDLSAYFGSAG"
     gene            complement(13554..14696)
                     /gene="hutI"
                     /locus_tag="VFO53_09060"
     CDS             complement(13554..14696)
                     /gene="hutI"
                     /locus_tag="VFO53_09060"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HET8876463.1"
                     /translation="MAVVVRDGGIAWIGAAAALPREFDAMPRHEGGDALVTPGLVDCH
                     THLVYGGDRAHEFGLRLAGASYAAIAARGGGIASTVRATREASEDALFEAASARLAAL
                     LADGVCAIEIKSGYGLDLANERKQLRVARRLADAWGVTVKATFLGAHAVPPEFTGRSD
                     AYIDCVCNDILPALAAEGLVDAVDAFCETIAFSPAQVERVFDAAVSLGLPVKLHAEQL
                     SDSGGAALAARYRALSADHLEHLSQAGVDAMRDAGSVAVLLPGAFYMLRETQLPPIDA
                     LREAGVPIAIATDHNPGTSPLLSLLLAMHMACTLFRLTVPEMIASVTRHAARALGLSS
                     THGTLGVGRPANFVLWDIRTTDELAYWLGRRPVAAIVRQGRIASRA"
     gene            complement(14846..16423)
                     /gene="hutH"
                     /locus_tag="VFO53_09065"
     CDS             complement(14846..16423)
                     /gene="hutH"
                     /locus_tag="VFO53_09065"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HET8876464.1"
                     /translation="MTIANAAAPRAGSRPTLTLGASPVTLGDLRRIYRDEVDVAIDDA
                     VVIPALAASQAIVQRIADGADAVYGINTGFGKLAQTRIAHERLAELQRNLVLSHSAGV
                     GAALAPGVVRLVLVTKAIGLARGFSGVRPEIVRALLALFNARVLPRIPCKGSVGASGD
                     LAPLAHLARILIGEGEVGAGDEAVTAAEGLRRAGVAPLVLGPKEGLALLNGTQVSTAL
                     ALAALFDAENVFAAALVASAMSLEAIKGSVRPLDARIHAARGQPGQIDVAAALRALLD
                     GSAIVPSHANCGRVQDPYSIRCVPQVMGACLDNMRHAARVLEIEANGASDNPLVFADT
                     AEVVSGGNFHAEPVAFAADILALAIAEIGAISERRVALLIDATLSGLPPFLVREGGIN
                     SGFMMPQVTAAALASENKSLAHPASVDSLPTSANQEDHVSMATFAARRLRDMLENTAV
                     IVGIEAMAAAQGIDFLRPLRSSDEIEREFAHIRSHVAFLAHDRHFAPDIDAMRGWALR
                     DEWPLAVTAVLPSRVRR"
     gene            complement(16420..17013)
                     /locus_tag="VFO53_09070"
     CDS             complement(16420..17013)
                     /locus_tag="VFO53_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HET8876465.1"
                     /translation="MIVGISGASGTVYGIRLLEALRELRFETHLVMTRSARLAMSYET
                     ALKPADVEALAAVNHRNEDVAASISSGSFRTLGMVVAPCSIRSLSEIATGVTSSLLTR
                     AADVVLKERRRLVLMVRETPLHLGHLRSMAQVTEIGAIVMPPVPALYNRPQSVDDIID
                     HSVGRVLDLFGIDNTLVRRWGEADGEPRDPAVTGRKA"
     gene            complement(17192..18205)
                     /locus_tag="VFO53_09075"
     CDS             complement(17192..18205)
                     /locus_tag="VFO53_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876466.1"
                     /translation="MARSIAAVAHTLAVAAFAAVCSGAVFIADAAAKDLVPVSVRLAF
                     NYNGHRSPYLLGVDKGFYADEGLDVKVLEGKGVTSSMQLVASKDDTFAIVDPPSLMLG
                     VAQGMPLEQVVQLYQVSPNALASWTSANIRKPADLAGKTVATLQGDTTTTMLYALLAK
                     NGVDAKQVKIVASDGGTRNQTFLSKRADAITGFTNDSYLSLRATADAPMSVFAYSDFG
                     IDTMGDGVAAHIDTIRSRPDVVRGFVKATLRAYRYALEHPDEAIASLAKRSPSIKRDV
                     EIEKLKATAPLLESADTRAHGIGYSSKDKWVTTQQLMLEFGGLKKAVDDVATYYTNDF
                     LPK"
BASE COUNT         2988 a         6272 c         6142 g         2963 t
ORIGIN      
        1 gaagctgtcg tactggtccg ccttcgccgc gcgcgcctcc ttgtccaccg tcttcgctgc
       61 gacgtcggcc cggagccgct ggaggcggtc accgccactc gcggagatgg cctgcttgag
      121 ctcgtcacgt cgtacttgct ccgcgcggcg gcgcgcctcg agctccgtga ggcgcgcggc
      181 gaggtagtcg cccatctcgc ggcttcgcgc gagcaggttc tcctgcgcga ccgtctcgag
      241 caccgcgagg ccgaccgtcg atggcagcgg atcggatacg tgcgacgtat agaacgcgaa
      301 gccgcgacga tggcacgtgg cttcgatctc gtcgttggtg atcaccgcag ccagcggcac
      361 gccgccaccg agcgtcttcg acacggtcat caggtcgggc gtgacgccga gctgcgacgc
      421 cgcgaaccag tgaccgaggc gtccgaacgc cgtctgcgcc tcgtcgaaga tcagcagcat
      481 gttgcgcttg cacgcttcct gctgcagcgc gtcgaaatag ccttcgggcg gcacgatcac
      541 accgccggca ctgatcaccg gttcggcgat gaccgccgcg cgtgcgccgc tcgactgcat
      601 gtcgtacagc tcgaagccga ccttcatgca ggtgcggtcg cacttgtcgc ggcagtgcct
      661 caccggacag cgatacgcgt tcggctcggg aatcacgtac gtgccgggca tccccggtcc
      721 gtagcccttg cgatcgctgg cgaaggaaac gccgcccgca ttgccggtga cgccgtgcca
      781 ggagccgccg aggccgacga cctcgaagcc cccggtgtag agcttcgcca tcttgatcgc
      841 cgcctcgttg ctctcgctcc cggtgttcag gaacagcgac ttcttgagcg gcgcgggcag
      901 gatcttcgcg agggcgtcgg cgagttgcac gacgcttctc ggaatcatgc ccgaaaagag
      961 gtgcaatgca ttgtcgcagc tgcgcttgat cgccgcgacg atgttcggat gattgtggcc
     1021 gaccgtggcg cacatctggc cggatgtgaa atcgaggatc ctgcggcccg tggcgtcgac
     1081 gacccaggag ccctgcgcct tctcgacgat ctcgggatag gcgtcgccgc cgtagcggat
     1141 caggtgctcg cggaaatcac cgcccgcttc actcgattca ttcatcactc gttccttact
     1201 cgcatgaggt ttgccgcaga aagtacccgg gcgctggcgt cgatcggcgc tatgatcatg
     1261 cgccacgaaa gggagggcgc gcatggcagg gagcgatacg cgattgctgg aggaatggaa
     1321 agccaacctc gatctgctca agttctacga gacgctgaag cagcagcggt ttgcacattt
     1381 cctcacgatc cagacggcgt ttctggcggt gttcgggctg gtggcgaagg acgccgctac
     1441 cgtgttctcg ctcgtcacga tcgcgaccct cgccgtggtg ccgattccgc cgctgttcat
     1501 ttcattctat ttcatgcggc tcgaccggcg cggccgcgcg ttcgtcgata cggccaatac
     1561 gcggctgctg ctgatcgagc gcgagtggca gcagcgcttc cccgacgacc acttcgcgac
     1621 gtaccagcag caatacggcg tgctgtcgcg gcgcgaccaa accttgatcg cgcaatacgt
     1681 ccgcacccgg aatgttgccg gcgaccctta cccgatgctc gtcaatgcac cctcggcgca
     1741 tgcgtccgag acggcgatca tccggctgtt ctggtggctg tgggtgatcc tgttcagcgg
     1801 cttcgcgctg catctcgcct ggcacctcgc caatcccgga tggcgaccct aggattcacg
     1861 ccgtccgggc acgtcgacga tctgagcagc gtgtccggtt gccgcgagga aacgctcgag
     1921 ttgcgtgtgc ggaagcacca tcgtggcgtc gttcaccagc ggatgcgcct gcacggcgtc
     1981 ggcatcccac agccgccggt cgaggacgaa ttcgacggcg tgcgcggtat cgttcgcgag
     2041 cgcgagcagg ctgactgcgc ccggcgctac accgaggtat ttgcgcaggc gctcgggcga
     2101 agcaaacccg aggcggccgg taccgagcag gagtccgagc gaattgagat cgacggcgac
     2161 gtcatgggca acggtcagca ggatgtgccg cgcacccttc ttgtcgcgca ggaacaggtt
     2221 tttcgtcttg gcgcctggaa ggtccggcac cagccgcttc gattcctcga ccgtcatcac
     2281 cggcgaatgc tcgaagcgct tcgcgtcgat gccgtgatgg gcgagaaagg cggcgaggtc
     2341 catcggtccg atttgaccac accccgccgc ggtccgccga ccgacgcgaa acacgcgcgg
     2401 caagcatcca cgagccgccg tgactcgtgg gcttgccgcg tggtgtgccg ggtactactg
     2461 cgtcgccgtc ttgcagcttc cggtgttgat gcaggtcgag acgagtgcgc acgtcgcctg
     2521 ctggccgtcg atcgagcatc cgcagccgaa ctgcgagcac caccactgct ggctccgcgc
     2581 catcgtcttg taggcatcgg aatcggtggt gacgcgggtg gtcgtcgagg cacaggcgga
     2641 caacagtgcg cacaacgtga tggccaacag gctgccgagg ggaatgcgct tcatgattcg
     2701 tcacctttcc ttatcgatgg cttcggatgc gcccggactt ccgcgagagg aatcgcgttc
     2761 attagagatc gaggcgtcga tcgcagttcg catgcaacgc gagattttta ttgatgcgga
     2821 caactgcgcc gccggagcgc cggctgcatt cgcaattgtc gggtcatgca aatgcgaccg
     2881 cgcgacgccg cgtggcgtag ggatggcaac gcggacctaa tcgagcgcgc ggccgccaac
     2941 gcgcagcagc cgttcgggac cgagctcggc aagttcgttg atgccgagca ttgccatgtc
     3001 gcgctcgatc tccgcggaca gaagcgcgat ggcatgcgca acgcccgcct cgccgccgat
     3061 cgccgccgca taaccgaacg gccggccggc gaacacgaat ttcgcgccga gcgcgagtgc
     3121 cttcaggacg tcggtgccgc gacgcacgcc cccgtcgagc atcaccggaa cgtcgggaca
     3181 tgcagcgacg atgcccggca acacgcgcag cgcggccacg gcgccgtcca gttgccgccc
     3241 gccatgattg gaaacgatga tgccgtcggc gcctgcgtcg cgcgcgagcg tggcgtcggc
     3301 cttgtcgagg acgcccttga tcaccagcct gccgttccac atccggcgga ccctgcgcca
     3361 atgctcccaa tgcaggtttc cgcggtcgga atagtcgcgc aacaccgacg ccgacaggat
     3421 cggcgcgccg cgtgtcgcga agttgttctc gaaatgcggc atcccgtgcc ggccgagcgt
     3481 gcgcaggaac gtgccaaaga gccagcgcgg atgcgtgagc ccgtcccacg cgagacgcaa
     3541 cgtcggccgc agcggcgtcg aaaagccggt ccggatattg ttctcccggt tgccggccac
     3601 ctgcgaatcg acggtgacga cgagcgtgcg gaacccggcc tggccgacgc gctcgaccag
     3661 cgcgtcgatc gccggcacgt cgcccggcag gtaggcctgg aaccacgccg acggacaggc
     3721 cgaaaccacc tcttcgagac ggatcagcga cgatccgctc atgatcatcg gaacgttcgc
     3781 ccgctcggcc gcgctggcga gcacgaggtc gccgcgatat gcgtagagcg cgctgatgcc
     3841 gagaggcgcg attccgaacg gcgcggaata cgcatgtccg agcagcgtcg tctgcgccga
     3901 ccgctgcgac acgtcgacga gcatgcgcgg caaaaagccg aattcggcga acgccgcccg
     3961 attgtcggcg agcgaccagt cggtttcgac ggcgccggcg acgtaaccga atacgggtcg
     4021 cggcagatga cgccggcccg ccgcctcgaa gtcatcgagc gacagcaccc ggcgaaggcg
     4081 ccgcggaacg gcggcggcgc ttgcacgatc cccccacgct tgcggcttcg ccgcggcgct
     4141 catcgcgagc gcgtccgccg ccgacgctcg agtctttgca ccattgcgac cccgtgagcc
     4201 cctcgaaacg gggcacagtc tagcagcgcc ccggggcagc cgcgggcggc ccggggtagc
     4261 gagcgcagcg cgcggaatgc cgcccgaata gcagccttga caatgactgc ttaggcatct
     4321 atattcatgc catgacacct gcaatcgaca ccgccgctgt ctccgacctg cccgacacga
     4381 aaccgcggca cggcatcctg cgccttgtca accgcatccg cgtcgagctg gtggatgcgc
     4441 tggatcgaga gctcgccccc ttcgacattt cggcgccgca gctcttcgtg ctctcgtccg
     4501 tcgccaaccg cgaagccgac tccgccgccg gcctgtgcaa gtcgatctcg tacgatccgg
     4561 gcgcgatgac ccggatgatc gaccggctgc agcagaaggg cctcgttcgc cgggttccgc
     4621 atcccgatga ccggcgcgcg atgaatctcg agctcaccgt cgccggcaag gcgctgttcc
     4681 cgcaactcgt ttccgccaag gaccgcgtgc aggcgcagtt tctgcgcggc ttcagcaagg
     4741 acgatgcgca cgccctcgaa tcgctgctgc atcgaatgct cgcgaatcgc tgattttttt
     4801 tcggccgatg gttgcctggg catcaaccgc ccaagcaaat agttccaccg gtttcgaacc
     4861 tcccgaacaa aaccatgacc ctcccgatca cgcgcgccgc ccttgcctgc gcctgcgctt
     4921 gcgccttgct cgccggctgc gcaagcatga gcggtatcgc gccgcaggcc caatcacgca
     4981 acgcgaacga gctcgcgtcg cagcacgcgc tctctgccgt ctcgaccagc agtgccgcat
     5041 ggccgaaagc cgactggtgg aaggcgtatg gcgatccgca gctcgacgcg ctgatcgacg
     5101 aagcgcttgc acacagtccg acgctcgacg tggcggccgc gcgcacccgc aaggcgctcg
     5161 ccgtcgccga tgcgggccga agcgcgcttt caccgcggat cgacgcaagt gcatcgagca
     5221 cccgcgagca cttttccgag cacgggctcg tcccgccgcc gttcggcggc acgacgctga
     5281 cgctgaacca gatgcagctc gcgctgtcat gggatcccga cgtgtggggc aagaaccgct
     5341 ccgcgtacga ggcggcgctc ggcagcgcgc gcgccgcgca gatcgacgcg cacgcggcac
     5401 ggctgcaatt gtcgaccggt gtggcgcagg cgtatgtgca actgcagcgc gcgtacctgc
     5461 agcgtgacgt cgcgcaggca acgctcgccg cgcgcgaaaa ggtcgtcgcg ctcacccgcg
     5521 accgcaatgc cgccggcctc gactcgcgcc tggaggtcaa gcaggccgaa gcggcgctgc
     5581 ccgcgacgcg cgaagaaatc gctgcactcg atgagcgcat cggccttgcg cgcaatcaga
     5641 tcgccgcgct gctgggcgcg ggccccgatc ggggccttgc catcgcgcgg cccgccgcca
     5701 ccgcgcttgc cgccatcgcg ttgccgtcgc gcctccctgc ggaactgctc ggccgccggc
     5761 ccgacatcgt tgcgcagcgc tggcggatcg aggcggcgag caagaatgtc gaatcgcaga
     5821 aggccgagtt ctaccccgac gtgaatctgc tggccttcgt cggattgcag agcctcggcg
     5881 gcgcgagcct gctgaccgcc gcgagccgca tgctgggtgc gggtccggcg gtgacgctcc
     5941 cggtgttcga cgcgggccgg ctgcgtgccc aactcgcggc gaaggacgcc gaccacgacg
     6001 tcgccgtcga ccaatacaac cagaccctcg ccgacgcgat gcgcgaggtc gtcgaccaga
     6061 tcgtgtcgtt tagaagtctc gacgagcagc gcaggcagca gcgcgacgcc gcggcgacga
     6121 cgcgcgaagc ctacgacctt gcgctgatcc gctatcggga aggcatcggg aactacctgc
     6181 aggtgctgtc cgccgaacaa ccgatgcttg cccagcaaag cctcgacgcc gacctttccg
     6241 cccgcgcgct cgagctttcg atcaacctcg tgcgtgcgct cggcggcggc tacgagcccg
     6301 aaccggcgca ggtctcgatg acgacaggga atccaagatg agcgacgcca aacccacttc
     6361 ccgatccgcc gcgaacggcg cacgcaagcg atggctcgcg attgtcgcgg gtgctttcgc
     6421 gctgatcggc atcgcctatg gagcgtactg ggcgctcgtg ctgcgctacg agcagtcgac
     6481 cgatgatgcg tacgtcagcg gcaacgtcgt gcagatcacg ccgcagatcg ccggcaccgt
     6541 cgtccggatc gccgccgacg acacgcagtt cgtcaaggcc ggctcgacgc tcgtcgagct
     6601 cgacccggcc gatgcgaaga tcgcgctcga acaggccgat gcaaagctcg cgaagaccgt
     6661 tcgcgacgtg cgcggtctct ttgcgacgac ggcgcagctc aaggcgaacg tcgacatgcg
     6721 caacgccgag ctcgcgcgcg ccagcgaaga cctgaaccgc cgcgagcggc ttgcgcgctc
     6781 gggcgcggtg tcgggcgaag agctgcagca cgcgcgcgac gcgttgaccg gcgcgaaggc
     6841 gtcgctcagc gccgcacagc aggagctcgc cgcgaaccag gcgcgcgtcg atcgcacgag
     6901 cgtcgagaat catcccgacg tgctcgctgc cgccgcacag gtccacgacg cctatctcga
     6961 cgtcgcgcgc accgcgctgc cggccccggt atcgggctat gtcgccaaac gcgcggtgca
     7021 gctcggacag cgcgtggccc cgggggcgcc attgatggcg atcgtgccgc tcgaccaggt
     7081 gtgggtcgac gcgaatttca aggagccgca gctcgcgacg atgcgcatcg gccagccagc
     7141 aacgctgacc gccgatctct acggcggcaa ggtgcgctat cacggcaccg tcgtgggatt
     7201 cggtgcgggc accggctcgg cctttgcact gctgccggcc cagaacgcca ccggcaactg
     7261 gatcaagatc gtgcagcgcg taccggtgcg cgtcgcgctc gacgcgcagg agctgaccgc
     7321 gcatccgctg cagatcggat tgtcgatgca ggtcgaggtc gacacgcatc agcgcggcgg
     7381 cgatcggctg ccgcagattg cccgggcgac gaccggccat tccaccgaca tgtacgacag
     7441 cgtcgatgcc gccgccgcga agcgcgtgca cgcgatcatc gccgccaacg agcgtgattc
     7501 ggcgagcgag cgcgcagcgt cggtcgccac ggcgcacgtt gcgcgcgccg gcgcagcggc
     7561 ctccgctgcg cgccagctgg cggtgtcgag gccgtaatcg accgcgcgac cgcgtatgag
     7621 ccgcgccctt tccaccacgg atccgcaatc gcggcgcacg ccggcgccag ccgcgcccgc
     7681 ctcccacgag ccggcaccgc tgtcgggcgc aacgctcgcg ctcggcaccg tcgcgctgtc
     7741 gctcgcgacg ttcatgaacg tgctcgacac gtcgatcgcc aacgtgtcga tcccggccat
     7801 cgccggcgat ctcggcgtgt cgcccgacca gggaacgtgg gtgatcacgt cgttcggcgt
     7861 cgccaacgcg atctcgctgc cgctcaccgg gtggcttacg caacgctacg ggcaggttcg
     7921 gcttttcgtc ggctcggtgc tgctgttcgt gcttgcgtcg ttcctgtgcg cgctcgcacc
     7981 gacgctcggc ctgctgattg cgtttcgcgt ggtgcagggc gcggtggccg ggccgatgat
     8041 tccgctgtcg caatcgctgc tgctgtcgag ctatccgaag gcgaaggcgg gaaccgcgct
     8101 ggcgctgtgg ggcatcacga cgctcgtcgc gcccgtcgtg gggcccgtgc tgggcggctt
     8161 cctcaccgac aacatcgcgt ggccgtggat cttctacatc aacattcccg tcggcctcgc
     8221 cgccgtggcg gtcacgtgga tgatctatcg tccgcgcgag actccgaccg cgaagctgcc
     8281 gatcgacgcg atcggcctcg gtctcctcgt gctgtgggtg ggcgcgctgc agatcatgct
     8341 cgacaaaggg aaggatctcg actggttcgg gtcgaccgag atcacgatcc tcggcatcgc
     8401 ggcgctcgtc ggcttcgcac tgttcgtcgt atgggagctg accgaggagc atccggtcgt
     8461 ggatctgcgc cttttcggcc ggcgcaattt ctggacgagc tcgctcgcga tgtcgcttgc
     8521 gtatggcgcg ttcttcggca acgtcgtgct gctgccgttg tggctgcagc aatacatggg
     8581 ctacacggca acggaggccg gcctcgtgct ggcgccggtc gggttgcttg cgatcctgct
     8641 gacgccgttc gtgggcagga cggtgaaccg ggtcgatccg cgtctttttg ccaccggcgc
     8701 gttcctgatc ttcgcgctcg ttctctacat gcgctcgaag ttcaacacgt ccgccgatct
     8761 gtggacgctg atggtgccga cgatcatcca gggcgcggcg gtggcgatct tcttcatccc
     8821 cctcgtgtcg ctcagcctgt cggggctccc gcccaaccgg attccgtccg cctctggtct
     8881 cttcaacttc gcacgcatca cggcgggctc gttcggcacg tcgatcgcca cgacgttctg
     8941 ggaccatcgc gcgacgctgc atcacgcgca gatcgtcgag cggctcacgg aagcgaatcc
     9001 ggcgacgacg caggcgctcg cggcgatgca tgccaacggg ctcacgcccg agcagggcta
     9061 cgcggcgctc aaccgcctcg tcgaccagca ggcgttcatg ctgtccgcca acgacgtgtt
     9121 ctacgtttcg gcgctgctgt tcgtggcgct gatcggcgtc gtgtggcttg cgcgccccgc
     9181 caagggtgcg gcgggggccg gtgaagcggc ggcgggcgcg cattgatgcc atgaccggcg
     9241 cggcgagcga cgtcgtcggt gtctggctgt cgagcgatgg ccggatgcgc gtggatctga
     9301 aagcggacgg cacgttcgac gaggctcgtg cgggtagcgc acgcacgttt cacggcgtat
     9361 ggcgaagcga cggatcgcgc gtccactttc gcgatccggc caccggctac gaggcgagcg
     9421 gcgagctttg tgacggcgtg ctgcacgccg acggcgtcga attccgcaag gccgccggga
     9481 tcagcgccgg tgctgcgacg gacgccactt gaggaagcga cgctcgaaca gcgtgatgag
     9541 gccgatgccg gccgtggtga acagcgtgat cgacagcagc gcggcgaaca cgagcgcggt
     9601 gtcgagattg aactgtgccg aaagcacgag ctggccgatg ccgcggttgc cgccgatgaa
     9661 ctcgccgacg atcgacccga cgagtgcgag cggaaacgcg accttcagcg cgtcggtgat
     9721 gtacggcgtt gcgtgcatca gccgcaccat cgtgaacacg cggtagcgcg atcccccggc
     9781 gagccgggcg agatcgagga tatcgggctc gatttcaccg agccccgtca tcgagttgac
     9841 gaaaatcgga aacagcgcga gcaggaacgc gagcaggatc ttcgattcga cgcccagtcc
     9901 gaaccagatg acgaaaagcg gtgcgatcgc gatcttcggg atgctgttga acgcggcgag
     9961 aaagggcatg atcacgctct tcacgccttc gacgtagacg acgagcagcg cgagcaacac
    10021 gccgacgacc agcgcgatcg caagccccgc gagcgtggtc atcgccgtca cggcgaggct
    10081 cgcgacctgc agcatgaacg tctcggcgaa gcggctcgcg atcgccgacg gcagcggcag
    10141 cacgtaatcg gggatgttca cgagcggcac caggaactgc cacgcgagca ggaacgcgac
    10201 gagcgccgtc acggaccgca gcggcgcgac aaccgaagtg ggatcagaga cgactttccg
    10261 catgctgtca atcgggggtc agagacgact ttccgcatgc tgctcatcgt agctcgacgg
    10321 ttcgatgaaa gtcgtctctg accccatgaa agtcgtctcc ggcccctgtc caatgccgtt
    10381 gcgaacccgt gccgacaacg cgatgaatcg cgggtgctcg agcactttca cgtcgcgcgg
    10441 ccgcggcagg tccaccttca cgatatcctg gatgcggccg ggtcgcggcg tcatcacgac
    10501 gatcgtgtcg gacagcagca ccgcctcggt gatgctgtgc gtgacgaaca gcaccgtgtt
    10561 gcgattcgcg gcccagatct tctgcagctc gaacgccatc cgctcgcgcg tcatgacgtc
    10621 gagcgcgccg aacggctcgt ccatcaggat cagcggcggg ttcatcagca gcgcacggca
    10681 gatcgccgcg cgctgctgca tgccgcccga gagctcgtgc ggcagcgcat tctcgaagcc
    10741 gccgaggccg gcgagcgcaa tcagctcgag cgcgcgttcg cggtgcgccg gcgccttgcg
    10801 gcctgccacc tcggcgacga acaggatgtt cgcaagcgtc gtccgccagg gcagaagcac
    10861 cggcgactgg aagaccatgc cgacgttctc gatcggcccg gacacggcct tgccggccac
    10921 cgtgatgcgc ccgccgcttg gcgcaacgag ccccgccacc atccgcagca gcgtcgactt
    10981 gccgcatccg ctggggccca cgatcgacac gaactcgttg ccttcgacgg cgaaggccgc
    11041 acgctcgacc gcaaccccgg tgccgaacga cttgcggacc tcgacgagct ccagcgcgac
    11101 cgacaccgtt cagtgccccg acagcaccgg cgtgtagaga tccctcgcgt cgagcttcgt
    11161 gtcgagcttc atcgcggtct tgacgaggtc gatcgttcgc tgcatgcgct cgtcggttgc
    11221 aacaccatag ccgttcttct tcacgtaggg cgtttcgatc gacttgatcg tctggcgcca
    11281 ttgcgcgagc gtcgtgtcgt agttgagcgc gggattgtgc ttgaccatga tcttcgccgt
    11341 ctcctcggga tgcgcgaccg agtagtccac cgccttgcgc gtcgcctcgg cgaatcgctt
    11401 cgcagcgccc ggatcgctcg cgagcgtcgt gtccttcgcg atcaggaagt agccgaacac
    11461 gtcgtaaccc cagtcggcga acggcgcgaa gtagaccttc ttgccgcgcg gctcggcgat
    11521 cttctcgagc gccgggatgt cgccgtcgat cgacgcggtg aaaagatcgg tctgccccga
    11581 cagcagcagc gacgagtaga cgcccgggtc ggcggcgagc cactggatcg atgcgggatc
    11641 gattttcgcg cgatcgagca ggatcggcag gatcacgcgt gcgctgtcgg tctgcgacgc
    11701 cgcgattcgc tggcccttca gcgattcgag cgtgttgtac ggcttgagcg atgcaagccc
    11761 cgacgtgctg atgtcggtga acaccatgaa gctcctcacc ttcgcgccct tggccaccgc
    11821 ctgcaccacg gggcccgcct ccgccatgcc gtagtcggcc ttgccggtgt cgacggcggt
    11881 gatgacgaag ccgctccccg tcgccggcgc gatctcgatg tcgaagcctg cgtcgcggta
    11941 ataccccttg tcgaggccga cgaagaacgg cgaatgccgg ccgtggaacg tgtaattcgt
    12001 gaggaacgtg atcttcttga gcggcgcctg cgcctgcgcg ggtgcctggc ttgcgcatac
    12061 gacggcgcaa agcgcgacga tggcggaaac gaccatgcgg accttcatgc gggttctccg
    12121 ttgggtgatc gagcgatgac gagggaacgg tctacgacgt gcgacgccga gaacgaatca
    12181 gcctgcgctg ccgaagtatg ccgaaagatc gacgctatcg gcgccgggga tgcgcttgcg
    12241 ctcgaaatcc ttgcgccggt cgaacggcgc ggtcgcgtcg atccccatcc ggccccagtg
    12301 gtccttgtac gcgtcgcggt agaacgacgg tgtgtcggga atcacgatcg tatccttgtc
    12361 gggacgcgaa cgcgtgagca ccgcccacat gacgtcctcc acgtcgtaga tgtcaacgtc
    12421 ctcgtcgacg accgtgcaca tcttggccca cgtgggctcg gcgccgaaga ccgcgagcac
    12481 gacctggcgc gcgtggccct cgtattgcgg gcgtatcttg acgacgacgt tggtgacgaa
    12541 cggatggcag gtgacatcga cgatcccggg cagcaccgcg ttgatccgct ggaagatgtt
    12601 cgcggcgacc gacagctcga gcgtcagcac ttcctccgac gagccgcaca ggatgctgtg
    12661 gaagatcgcg tcggcgcgcc gcgtgacgcc aagcacctcg aacaccgcgt tctggcccac
    12721 cggcgtgtag taacccatga attcgccgaa gggcccctcg tcgcggcgca cgttcggcag
    12781 gaaccggccc tcgatcacga tctcggtcga cgcgggcacc atcatgtcga tatgcctgca
    12841 cggccgcatt tcgatcgatt cgccggcgag cttcgcggcg acctcgagct cgtcggcatc
    12901 gtaggggagc ggcgacgcgg ccgtcaggaa cgccgtcggc ggcggcccga tcagcatcgc
    12961 cgcctcgagc gggcggccca tcttctccgc cttctcgtgg tacatcgtca gatggtggcg
    13021 cggggcgagc cggcagcgca actcgtcgtc gctgacgaac atcgagcgat ggaacgacag
    13081 gttgcgcacg ccggtttccg gctcgtgcgc gaggaacatc gccgacgtga aatacggcgc
    13141 cgcatcgcgc tccgaatacg tgatcagcgg caggtccgag accttgcagt cgacgagatt
    13201 gccggtcgga cgcaaggcgg gccgcagcgc gacgtcgcgc gtgccggcga cgctcgcaag
    13261 gtcgttccac ttgcggcaga actcgtcggg cgcgatgccc agaatttccg ccaggcgccc
    13321 gcgcgagccg tagatgttcg tcagcaccgg caacgccgtg ccaccgacgc ggtgaaagag
    13381 gatcggcttg ccccaccgcc gatgcgctgc cgacgtgacg gccgcaagct cgtgcttcgg
    13441 atgcacctcg cgcgtcacct cgagcagttc gccgcgcgcc cgaagcgctt ccatgtactc
    13501 ccgcattgac gcttccatcg cgttcctcga atggttgact gaagaggtgt cggtcaggcg
    13561 cgcgatgcga tgcggccctg gcgcacgatc gcggcgaccg gccggcgccc cagccagtag
    13621 gcgagctcgt cggtcgtccg aatgtcccac agcacgaagt tggcggggcg gccgacgccg
    13681 agcgtgccgt gcgtcgacga aaggccgagc gcgcgcgccg catgccgcgt gacgctcgcg
    13741 atcatttccg gcaccgtcag gcggaacagc gtgcacgcca tgtgcatggc gagcagcagc
    13801 gaaaggagcg gcgaggtgcc gggattgtgg tccgtcgcga tcgcgatggg cacgccggcc
    13861 tcgcgcaatg cgtcgatggg cggcaattgc gtttcgcgca gcatgtagaa cgcgccggga
    13921 agcagcacgg cgacgctgcc cgcgtcgcgc atcgcgtcga cgcctgcctg cgacaggtgc
    13981 tcgagatgat cggcggaaag cgcgcggtag cgcgcggcga gcgctgcacc gcccgagtcg
    14041 gaaagctgct cggcgtgcag cttcaccggc agcccgagac tcaccgcggc gtcgaacacg
    14101 cgctcgacct gcgccggcga aaacgcgatc gtctcgcaga acgcatcgac ggcgtcgacg
    14161 agcccctcgg cggcgagcgc cggaaggatg tcgttgcaca cgcagtcgat gtaggcgtcg
    14221 cttcgtccgg tgaactcggg cggcaccgca tgcgcgccga gaaacgtcgc cttcaccgtg
    14281 acgccccagg cgtcggcgag ccggcgcgcc acgcgcaatt gcttgcgctc gttggcgagg
    14341 tcgagcccgt agccggactt gatctcgatc gcgcaaacgc cgtccgcgag aagtgcggcg
    14401 agccgcgccg acgcggcttc gaacaacgcg tcctcgctcg cctcgcgcgt cgcgcgcacg
    14461 gtcgacgcga tgccgccgcc gcgcgccgcg atcgcggcgt agctcgcgcc cgccagacgc
    14521 aggccgaatt cgtgcgcacg atcgccgccg tagacgagat gcgtgtggca gtcgacgagt
    14581 cccggcgtca cgagcgcgtc gccaccctcg tggcgcggca tcgcgtcgaa ctcgcgcggc
    14641 agcgccgccg cggcgccgat ccatgcgatg ccgccgtcgc gcaccacgac cgccatgttc
    14701 gtcgatgatg cgatcgcccg gtcgtcgagc gtgctgtcga tgacgcgcaa tcgatgccag
    14761 acgccctcgg cgcttctccc ctcaccctcc ccctgctcgc tgggagaggg agccgagcgc
    14821 gagcgagacg ggtcaggggt cgagttcatc ggcgtacgcg gctcggcagc acggcggtga
    14881 ccgcaagcgg ccattcgtcg cgcaacgccc agccccgcat cgcgtcgatg tccggcgcga
    14941 aatgccggtc gtgcgcgagg aacgcaacat gcgagcggat gtgcgcaaat tcacgctcga
    15001 tctcgtccga gcttcgcagc ggccgcagga aatcgatgcc ctgcgccgcc gccatcgcct
    15061 cgatgccgac gatgaccgcg gtgttttcga gcatgtcgcg aagccgccgc gcggcaaacg
    15121 tcgccatcga gacgtgatct tcctgattcg ccgaggtggg caggctgtcg acgcttgccg
    15181 gatgcgcaag cgacttgttt tccgaggcga gtgccgcggc cgtcacctgc ggcatcatga
    15241 agcccgaatt gatgccgccc tcgcgcacga ggaacggcgg cagcccggac agcgtcgcat
    15301 cgataagcag tgcgacgcgc cgctcggaga tcgcgccgat ttcggcgatc gcgagcgcga
    15361 ggatatccgc cgcgaatgcc acgggctcgg cgtggaagtt gccgccggag acgacttctg
    15421 ccgtgtcggc gaagaccagc ggattgtcgg atgcgccgtt cgcctcgatc tcgagcacgc
    15481 gcgctgcatg ccgcatgttg tcgaggcacg cacccatcac ctgcggcacg caacgaatcg
    15541 aatagggatc ctggacgcgg ccgcagttcg cgtgcgacgg cacgatcgcg ctgccgtcga
    15601 ggagtgcccg caaggcggcg gcgacgtcga tctggccggg ctgcccgcgc gcggcgtgaa
    15661 ttcgcgcatc gagcggacgc accgagccct tgatcgcctc gagcgacatt gcgctcgcga
    15721 cgagcgcggc ggcgaacacg ttctccgcgt cgaacagcgc ggcgagtgcg agcgccgtcg
    15781 atacctgcgt gccgttcagc agcgcgagcc cctccttcgg cccgagcacg agcggcgcaa
    15841 cgccggcgcg gcgcaggccc tcggcggccg tgaccgcttc gtcgccggcg ccaacctcgc
    15901 cctcgccgat caggatgcgg gcgagatgcg cgaggggcgc gaggtcgccc gacgcgccga
    15961 ccgatccctt gcacggaatg cgcggcagca cgcgggcatt gaagagcgcg agcaacgcgc
    16021 gaacgatttc gggacgcacg cccgaaaagc cgcgggcgag gccgatcgcc ttggtgacga
    16081 ggacgagccg cacgacaccc ggcgcaagcg cggcaccgac gcccgcgctg tgcgagagga
    16141 cgagattgcg ctgcaactcg gcaaggcgct cgtgcgcgat gcgcgtctgc gcaagcttgc
    16201 cgaatcccgt gttgatgccg tacaccgcgt cggcgccgtc ggcgatccgc tgcacgatcg
    16261 cctgcgacgc ggcaagcgcc ggaatcacga ctgcgtcatc gatggcgaca tcgacttcgt
    16321 cgcggtagat gcggcgcaga tcaccgagcg tcaccggcga cgcgcccagc gtgagcgtcg
    16381 gccgcgatcc ggcgcgcggc gcggcggcat tggcgatcgt catgcctttc gccccgtcac
    16441 ggcgggatcg cgcggctcgc cgtccgcttc tccccagcgg cgcaccagcg tgttgtcgat
    16501 gccgaacaga tcgagcacgc gtccgacgct gtgatcgatg atgtcgtcga cggattgcgg
    16561 ccgattgtag agcgccggga ctggcggcat cacgatcgcg ccgatctcgg tgacctgcgc
    16621 catgctgcgc aggtgcccga ggtgcagcgg agtctcgcgc accatcagca cgaggcgacg
    16681 ccgctccttc agcacgacgt cggcggcgcg cgtgaggagg ctcgacgtca cgccggttgc
    16741 gatctcggac aggctgcgga tcgaacaagg cgcgaccacc atgccgagcg tgcgaaacga
    16801 gccgctcgag atcgacgccg cgacgtcctc gttgcggtga ttgaccgcgg cgagcgcctc
    16861 gacgtcggcg ggcttgagtg cagtctcgta cgacatcgca aggcgcgccg agcgcgtcat
    16921 cacgaggtgc gtctcgaacc ggagctcgcg cagtgcctcg agaagccgga tgccatagac
    16981 ggtccccgac gcgccgctga tgccgacgat gattcggtga cccccccacg cttgcggtgg
    17041 attggacgat gcgcgtgctt cgcccgcgcc tgttcgccct tggggcggcc catcgggctc
    17101 acgcgcggcg ctgccccccg cgggggcgga attcgcgcct cggggcggcc cttcggcgct
    17161 catgcgccgg cgcgccggat gctcgcgcgc gctatttcgg gaggaagtca ttggtgtagt
    17221 acgtcgcgac gtcgtcaacc gccttcttga ggccgccgaa ttcgagcatc agctgctgcg
    17281 tcgtcaccca cttgtccttg ctcgagtagc cgatgccgtg cgccctggta tccgccgatt
    17341 cgaggagcgg cgccgtcgcc ttgagcttct cgatctcgac gtcgcgcttg atgctgggcg
    17401 agcgcttcgc gagcgacgcg atcgcttcgt cgggatgctc gagcgcgtaa cgataggcgc
    17461 gcagcgtcgc cttgacgaat ccgcgcacca cgtcgggcct cgagcggatc gtgtcgatgt
    17521 gcgcggccac gccgtcgccc atcgtgtcga tgccgaagtc cgaataggcg aacaccgaca
    17581 tcggcgcatc cgcggtcgcg cgcaacgaca ggtacgaatc gttcgtgaag ccagtgatcg
    17641 cgtcggcgcg cttcgacaag aacgtctggt tgcgcgtgcc gccatccgac gcgacgatct
    17701 tgacctgctt cgcgtcgacg ccgttcttcg cgagcagcgc atagagcatc gtcgtggtcg
    17761 tgtcgccctg cagcgtggcg accgtcttgc cggcgaggtc cgcgggcttc ctgatgttcg
    17821 ccgacgtcca gctcgcgagc gcgttcgggc tcacctggta gagctgcacc acctgctcga
    17881 gcggcatccc ctgcgcaacg ccgagcatca gcgacggcgg atcgacgatc gcgaacgtgt
    17941 cgtccttgct tgcgacgagc tgcatcgacg acgtgacgcc cttgccttcg agcaccttca
    18001 cgtcgaggcc ctcgtcggcg tagaagccct tgtcgacgcc gagcaggtac ggcgagcggt
    18061 gtccgttgta attgaacgcg agccgcacgc tgaccggcac gagatccttc gccgccgcat
    18121 cggcgatgaa gacggctccg gaacagacgg cggcgaacgc cgccacggcc agggtgtgcg
    18181 caactgcggc gatgcttcga gccattgctt cctcctcgtt ggttatcgcg attcctgcag
    18241 tattcctgcc tggacgaaca gcgtccgcac gtgcgcgacc agcgcgagaa agcgcggtga
    18301 ttcgcgcgtc tcgaccgtgc gcggccgcgg caggtcgacg tcgacgatct cgtcgatgcg
    18361 ccccg
//
LOCUS       DASSVX010000211         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1325356, whole genome shotgun sequence.
ACCESSION   DASSVX010000211 DASSVX010000000
VERSION     DASSVX010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325356"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1821)
                     /gene="fusA"
                     /locus_tag="VFO53_09080"
     CDS             complement(<1..1821)
                     /gene="fusA"
                     /locus_tag="VFO53_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET8876467.1"
                     /translation="MADYATQNVRTVAWVGDGGAGKTSLVEALLAKAGAIPAQGSVER
                     GSTVSDFDPLEKTYQHSLRASLVHLETDGTRIHMIDTPGLPDFIGQAIGALDAVETAA
                     IVVNASTSVQMIGARMMQWAAERKLCRLVVINRIDAENVDPPAVLDAVRNAFGKECLP
                     INLPAENGKRVVDCFFNPSGESDFSSVEDAHRALVDQVVEVDEELMSLYLEQGEVAPE
                     QLHAPFEKALREGHLVPVCFVSARTGAGVAELLDVLVKLAPNPTEGNPPLFTKGEGDS
                     ATQFHSEPDPRKHVLAHVFKVVMDPFVGKLGIFRVHQGTVTRDTQLFVGDGRKSFKVG
                     HLFMLQGAKYVEIDRAVPGDLAAVAKVDEVVFDCVLHDSHDEDQIHMRPLEFPVPMHG
                     FAITPKRRGDEQRISDVLHKMTAEDPTLVVGHDPVVNETVLRGLGELHLRSVFERMAS
                     QFKLEVETRPPRVPYRETVTAKAEGHHRHKKQTGGAGQFGEVYLRIEPLPRGSGFEFV
                     DQVKGGAIPNTLIPAVQKGVEQVLQAGPVAGFPLQDVRVTVYDGKHHDVDSKEVAFVS
                     AGRKAFLDALAKARPIVLEPIVNVDIVAPEEKMGDITGELS"
     gene            2010..>2244
                     /locus_tag="VFO53_09085"
     CDS             2010..>2244
                     /locus_tag="VFO53_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET8876468.1"
                     /translation="MSRAARKIASDPLCFLKQTVRAFRANQGLLLAGGVAYYTLLSIV
                     PLMILLVIILSHVIEAEALLATLARYLEIVAPGQ"
BASE COUNT          352 a          762 c          732 g          398 t
ORIGIN      
        1 ggaaagctcg ccggtgatgt cgcccatctt ctcttccggc gcgacgatgt cgacgttgac
       61 gatcggctcg aggacgatcg gcctcgcctt ggcgagcgca tcgagaaaag ccttgcggcc
      121 ggcggaaacg aacgccacct ccttcgagtc gacgtcgtgg tgcttgccgt cgtagacggt
      181 cacgcgcacg tcctgcagcg gaaagccggc gaccggcccg gcctgcagca cctgctcgac
      241 gcccttctgc accgccggaa tcagcgtgtt cggaatggcg ccgcccttca cctgatcgac
      301 gaactcgaag cccgagccgc gcggcagcgg ctcgatgcgc aagtacacct cgccgaactg
      361 accggcgccg ccggtctgct tcttgtgccg atgatggcct tcggccttcg ccgtgaccgt
      421 ttcgcggtac ggcacgcgcg gcggccgcgt ctcgacctcg agcttgaact ggctcgccat
      481 ccgctcgaac accgagcgca gatgcagctc gcccaatccg cgtaacacgg tctcgttcac
      541 gaccggatcg tgtccgacga cgagcgtcgg atcctcggcg gtcatcttgt gcagcacgtc
      601 ggagatccgc tgctcgtcgc cacgccgctt cggcgtgatc gcgaagccgt gcatcggcac
      661 cgggaattcg agcggccgca tatggatctg gtcctcgtcg tgcgagtcgt gcagcacgca
      721 gtcgaagacg acctcgtcga ctttcgcgac cgcggccagg tcgcccggca ccgcgcggtc
      781 gatctcgacg tacttcgcgc cctgcagcat gaagaggtgc ccgaccttga acgacttgcg
      841 gccgtcgccg acgaagagtt gcgtgtcgcg cgtgacggtg ccctggtgca cgcggaagat
      901 gccgagcttg ccgacgaacg ggtccatgac gaccttgaac acgtgcgcga gcacgtgctt
      961 gcgcggatcg ggctcggaat ggaattgcgt ggcgctgtcg ccttcgcctt tggtgaagag
     1021 cggcggattg ccctcggtgg gattcggcgc gagcttgacg agaacgtcga gcagttccgc
     1081 aacgcccgcg ccggtgcgcg cggacacgaa gcagaccggc acgagatggc cttcgcgcag
     1141 cgccttctcg aacggcgcat gaagctgctc gggcgccacc tcgccctgct cgaggtaaag
     1201 cgacatcagt tcctcgtcga cctcgacgac ctggtcgacg agcgcgcggt gcgcgtcctc
     1261 gacggaagag aagtcggatt cgccgctcgg attgaagaag cagtcgacga cgcgcttgcc
     1321 attctcggcg ggaagattga tcggcaggca ctccttgccg aacgcgttgc gcaccgcgtc
     1381 gagcaccgcg ggcgggtcga cgttctcggc atcgatccgg ttgatgacga cgagacggca
     1441 cagcttgcgc tcggccgccc attgcatcat tcgcgcaccg atcatctgca cgctggtcga
     1501 cgcgttgacg acgatcgcgg cggtttcgac cgcatcgagc gcgccgatcg cctggccgat
     1561 gaaatcgggc agccccggcg tgtcgatcat gtggatgcgc gtgccgtcgg tttcgagatg
     1621 cacgagcgac gcgcgcagcg agtgctggta cgtcttctcg agcgggtcga aatcgctgac
     1681 cgtgctgccg cgctcgacgc tgccctgcgc aggaatcgcg ccggccttcg cgagcagcgc
     1741 ctcgacgagg gacgtcttgc ccgcgccccc gtcgcccacc cacgctacag tacgaacgtt
     1801 ctgggtcgcg taatctgcca tcgtcgtctc ctccgtaagg atgggaggaa tgtaccgccg
     1861 atgcccggcc ctgtcagcca cggccggcgt tgcgcacgcc gcgttgcgcc tttgtcctac
     1921 gtgctcgcgg ggcggtgcgc cgacagcggg tcccgtgcga acatgtgagc atcgaccacg
     1981 tgttcgcgag cctcgccctg cgaccgggaa tgtcacgcgc tgcgcgcaag atcgcgagcg
     2041 atccgctttg cttcctgaag cagacggtgc gcgcattccg cgcaaaccag ggtctgctgc
     2101 tcgccggtgg cgtcgcctat tacacgctgc tgtcgatcgt gccgctgatg atcctgctcg
     2161 tgatcatcct gtcgcacgtc atcgaggccg aggcgctgct cgcgacactc gcgcgctatc
     2221 tcgagatcgt cgcacccggc cagt
//
LOCUS       DASSVX010000212        15116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_858763, whole genome shotgun sequence.
ACCESSION   DASSVX010000212 DASSVX010000000
VERSION     DASSVX010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 15116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15116
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858763"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /gene="rlmD"
                     /locus_tag="VFO53_09090"
     CDS             complement(<1..1310)
                     /gene="rlmD"
                     /locus_tag="VFO53_09090"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET8876469.1"
                     /translation="MTELTIESLDQEGRGIAHAGGKVVFVEGALPGERVDAEVVRRKP
                     SYEIARVAAVHGASASRIPPRCPHFGVCGGCTLQHADDALQLAAKQRALEDALARIGR
                     VRPGVMLPAIAGPAWGYRYRARLSVRDVPKKGGVLIGFHERKSSYVADMRECHVLPPK
                     ISALLPALRTLVESLSIRTRLPQIELAVGERLAPRALAEVTGGTDERLVHALVLRVLA
                     PPSVSDEAKLVAFADAHDVEFWLQPGGPDTVLPFHPAHASLAYTLPEFDVAIPFAPTD
                     FTQVNASINRVLVRRAVQLLDPQPADRVADFFCGLGNFTLPIARRVRHVTGVEGSKAL
                     IARATANAERNRLASTTTFVCANLFTATPAFIASLGVLDRVLVDPPREGAVALAKSLP
                     GCDDARALKRIVYVSCNPATLARDAAVLVHERGYRLAAAGVVNM"
     gene            1368..2057
                     /gene="tsaB"
                     /locus_tag="VFO53_09095"
     CDS             1368..2057
                     /gene="tsaB"
                     /locus_tag="VFO53_09095"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET8876470.1"
                     /translation="MKILALDTSTRWLSVALFDGKHACTIRECVDTAASQRILPAIDE
                     ILTRDGTPLASVDGIAFGAGPGAFTGVRIACAVAQGLAFGAERPVFAVGTLEAIAQSA
                     WREHGWERVVACLDARMHEVYVAAYERDRNGWMLVREPAVDKPGAVDPFAGGWHGAGD
                     AFAAYSGLAERLGLAAYDGAIIPDAQSVAECAWPRVLAHAGVPAHEAQPLYVRHRVAL
                     TSAERAAGVRL"
     gene            2068..2574
                     /gene="rimI"
                     /locus_tag="VFO53_09100"
     CDS             2068..2574
                     /gene="rimI"
                     /locus_tag="VFO53_09100"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET8876471.1"
                     /translation="MASLPKPTPLAHVRWRPLAAHDLPVVAALEARIHAAPWSERNFR
                     DALAAGYSARVGMRAERIVAYGVLMLGPGEAQLLNLSVVPEARRGGLGRALLLQFMDV
                     ARQLGAEHLFLEVRVGNLGAIALYRAEGFAAIARRVEYYPADKRGGVREDALVMRCDL
                     RGSAASVT"
     gene            2574..3293
                     /locus_tag="VFO53_09105"
     CDS             2574..3293
                     /locus_tag="VFO53_09105"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET8876472.1"
                     /translation="MPTRNEVLRELGIAPLWRLRERTRTDAPRGDEAADEPRDANRDR
                     RLAHIAALALDALVDDVAQCRACGLCATRRRTVPGIGAADAAWMFVGEAPGADEDAQG
                     EPFVGAAGRLLDNMLAALGLARDRDVFIANVLKCRPPGNRTPAPLEVDACRPYLDRQI
                     ELVGPGLIVALGKSAACALLGVDASVASLRNRVHRYRGHPLIVTYHPAYLLRTPADKA
                     KAWEDLLFARRTLRESVPPPV"
     gene            3399..4091
                     /locus_tag="VFO53_09110"
     CDS             3399..4091
                     /locus_tag="VFO53_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET8876473.1"
                     /translation="MLRKLGVVLAAVITMALGGCGYNDLQRQDEGVKASWSEVLNQYQ
                     RRADLVPNLVNTVKGYAQQEQQVLTEVTNARASVGAIKATPELINDPAAFQKFIQAQN
                     QLQGALSRLLVVSENYPNLKSDAAFRDLQAQLEGTENRIAVARNRYIKTVQEFNSTVR
                     QFPTNLTAMIFKMNVKPNFSVADEGAVSTPPKVDFGKGAAPVVPPPGGATPPSPGPAP
                     APAPPPAPAPKS"
     gene            4150..5109
                     /locus_tag="VFO53_09115"
     CDS             4150..5109
                     /locus_tag="VFO53_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HET8876474.1"
                     /translation="MIASLVRIDSSPWRRGFAGMVALVMLFVACAAAGQGKAAWETDR
                     SGLAPIPKLTARVTDETSTLSPAERQALEAKLAAWEQNSGNQLVVLIVASTKPEPIES
                     YSIRVADAWKIGRKGQDNGAIFVVAKDDHKMRIEVGYGLEGVLTDLTSHQIIDETVAP
                     LFRQNKFAEGINAGVDRIIAVVGSGAPLPAKAATQRARQPFDFGTLLIFLFVGVPIVG
                     GILSRIFGRMLGSTIGGGAVGAAAWLIAGSIAIGVGAGVVALLIMLLFSAGGGIGRRG
                     GMWIPTGGWGGGGLGGGGFGGGGGGGFSGGGGSFGGGGASGGW"
     gene            5112..5609
                     /locus_tag="VFO53_09120"
     CDS             5112..5609
                     /locus_tag="VFO53_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET8876475.1"
                     /translation="MRHRRLLRHFVTDHLSVKRAFPPAALALIEQAIAAGERTHRGQV
                     CFAVEGALPPLRVLRRLTPRERALEVFGLLRVWDTEENAGVLIYLLLADRDVEIVADR
                     GIDRQVPPGAWQAICARMEAAFRDAHLVEGVVVGIEEIGALLATHFPRIGAVANELPD
                     KPVVL"
     gene            complement(5629..6894)
                     /gene="lplT"
                     /locus_tag="VFO53_09125"
     CDS             complement(5629..6894)
                     /gene="lplT"
                     /locus_tag="VFO53_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HET8876476.1"
                     /translation="MKPGFYTIIAAQFFSSLADNALLIAAIALLRELHEPAWMTPALK
                     QSFVVSYVVLAPLVGAFADSMPKGRVMLITNAIKIVGCAMMLADVHPLLSYAMVGFGA
                     AAYSPAKYGILTELLPPQQLVIANGWMEGATVGSIIFGFVLGGALITPRVSAALLGFD
                     FPMIDLPIDTPPDAAIAVIMAIYLIAAIFNWYIPDTGVDHRVPSKNPLYLIREFSHCV
                     SLLWRDRLGQISLATTTLFWGAGATLQFIVIDWAARALSLNLSQASMLQGVVAVGVAL
                     GAILAARFVSLRGAVNVLPIGAAMGVVVLVMVFVQYVPLAMVLMVAIGACAGFFVVPM
                     NALLQHRGHVLMGAGHSIAVQNFNENIGVLMMVGLYALMVRAGISVSTAIMLFGMFVT
                     GTMLLVMWRHRANQREYDSMHLIGIDKPS"
     gene            7087..8172
                     /gene="alr"
                     /locus_tag="VFO53_09130"
     CDS             7087..8172
                     /gene="alr"
                     /locus_tag="VFO53_09130"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET8876477.1"
                     /translation="MARPLVAHIDTGALAANLACARALAAGASVLAVVKADAYGHGLA
                     RVLPALTAADGLALVELDAAVRLREHGERRPILLLEGFFEPAELDALAAHSLATVVHC
                     ADQVDLLEKARVSRPLDVYLKVNTGMNRLGIAPADVRALAQRLSSARSVASLHLMTHF
                     ARADEPDAIAEPLARFAAACAGLPYPRSLANSAGVVRYADVGGDVVRPGVMLYGATPF
                     AARSAQALGLSPVMTLRSKLIGVQTLAAGESVGYAATFTAARPMRVGIVACGYADGYP
                     RHAPNGTPVLVDGRRVPMAGRVSMDMLTVDLSSVPQAGIGSDVVLWGEGLPVDDVAKA
                     AGTVGYELLCAVAPRVRFEVHEPAGAA"
     gene            8201..9208
                     /gene="rfaD"
                     /locus_tag="VFO53_09135"
     CDS             8201..9208
                     /gene="rfaD"
                     /locus_tag="VFO53_09135"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HET8876478.1"
                     /translation="MYVVVTGAAGFIGSNLVQGLNARGETDIIAVDDLTHGDKFRNLV
                     DCRIADYIDKDEFLAGIADGDFDDDIAVIFHQGACSDTTASDGRYVMRNNYRYSVALL
                     EHCQDNDIPFLYASSAAVYGSGSVFREERAHEAPLNVYGYSKSLFDDYVRRVLPERTA
                     PIAGFRYFNVYGPREAHKGRMASVAFHFFGQYRRDGRVQLFEGSEGYGNGEQRRDFIH
                     VDDVVAANLHFYGRPERAGIFNLGTGEAATFNAVAHATVNACRAAAQDAPRSLEQLVA
                     DGIITYVPMPEVLRGKYQSFTQADVTRLRAAGYVAPMLTVAEGVPRYVESLISAAGPR
                     S"
     gene            9290..9613
                     /locus_tag="VFO53_09140"
     CDS             9290..9613
                     /locus_tag="VFO53_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET8876479.1"
                     /translation="MIRLLLALVVALATPLADAALNLNTATKDELVALSGIGPAKAQA
                     ILDYRVQHGGFKSVDELKDVKGIGARRFEKLKPELTVVPAPVKTAAREAKPAAAAKSE
                     AKSNK"
     gene            9801..10454
                     /locus_tag="VFO53_09145"
     CDS             9801..10454
                     /locus_tag="VFO53_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET8876480.1"
                     /translation="MKRLLLAFVLLFAASFAFAAVNLNTATKEELDALPGIGPVKAQA
                     IIDYRNANGPFRTPEDVMKVRGIKEGEFGKIRDQISVSGSTTPVQRPAAATTAPATAA
                     PRAAAPAPATPAAAPAPARTAAPASTATAPASAAPAKADNAKMTKAEEKAARAKAREE
                     KAARAKEEKAAKAKEKQEKAAKAKEDKTAKAEATKTKASAKDKAAAETSAKDVKPKP"
     gene            10547..11437
                     /gene="cysM"
                     /locus_tag="VFO53_09150"
     CDS             10547..11437
                     /gene="cysM"
                     /locus_tag="VFO53_09150"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HET8876481.1"
                     /translation="MSYATLADAVGNTPLVRLMRLPGATSNVILAKLEGNNPAGSVKD
                     RPALSMIVEAEKRGTIKPGDMLIEATSGNTGIALAMVAAMRGYRMLLVMPESQTAERV
                     QTMRAYGAELVLTPKAGGMEAARDIAMRMRDEGRGVILDQFANPDNPLAHYRGTGPEI
                     WRDTAGRVTHFVSAMGTTGTIMGVSRFLKEQNPAITIIGAQPEEGSSIPGIRKWAPAY
                     LPKIFDASRVDRTENVSQADAEDHARRLARDEGIFCGISSGGACAVALRLSQGIENAT
                     IVFIVCDRGDRYLTTGVFPA"
     gene            11500..12291
                     /locus_tag="VFO53_09155"
     CDS             11500..12291
                     /locus_tag="VFO53_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET8876482.1"
                     /translation="MIPLLVFDIETVPDIAGIRRIHEIGDAVDDAGVLDWFLQRRRAA
                     SGSDFAPLHLQKVVAIACAMRDTAGIRIWSIGDPADDEPELIRRFFDGIEKYTPQLVS
                     WNGGGFDLPVLNHRALIHGIAASRYWDWGDDDKEFRYNNYLGRYHTRHLDLMDVMAQY
                     QLRASAGLDAMARLCGFPGKLGMDGSEVAAAIAAGRIADVRSYCECDVLNTYLVYQRF
                     RLMRGEMDAGEYAREVSLVRERIGASDAAHWRAFIAQWDGASVTA"
     gene            complement(12281..13120)
                     /locus_tag="VFO53_09160"
     CDS             complement(12281..13120)
                     /locus_tag="VFO53_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET8876483.1"
                     /translation="MTDYAFRIVNVFAETPLAGNPLAVFEDARGLDDATMQALALQFN
                     LSETTFVLPSQAATRRVRIFTPTFEMPFAGHPTLGTAHVVRTLADAGDQVTLQMHAGV
                     IPVHADGDVWTLTANAPRHRASSASRSELAAMLGLSIDDLAAADPLWVDTGSEQFIVP
                     LASFDAVRRARPSPAQMLLHASNGQRAMAYVFARDGDHVVSRFFFPKHGAMIEDPGTG
                     SACANLGGWLLATGARLPQRLTIDQGEAVGRPCRLGLFVDSQCSIHVSGRVIEIGRGS
                     ITL"
     gene            complement(13117..14214)
                     /gene="mnmH"
                     /locus_tag="VFO53_09165"
     CDS             complement(13117..14214)
                     /gene="mnmH"
                     /locus_tag="VFO53_09165"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HET8876484.1"
                     /translation="MIAPLPPGPPLRAETRASVDDFASFRDRIDVRSPGEFAEDHLPG
                     AINLPVLDDEERARVGTTYVQVSAFDARKAGAAIVARNIAAIVATHVQDKPRDWSPLV
                     YCWRGGQRSRALVHVLNEIGFRAVQLDGGYRAYRRHVVALLAQWPRQFRYRVVCGFTG
                     SGKSRLIEAIAAEGGQVLDLERLACHRGSLLGDIPGAPQPTQKAFESALLESFARFDA
                     SRPVFVESESRRIGRLQVPDALLESMREAPCIRLETSTSNRVVMLASDYAHLASDPVA
                     FTAQLALLASLHGNAVLARWQAMMESGAVRELATELLDVHYDPSYERAIARNFPHYRN
                     AVVLHVDDARDETFRGLARQLLGALPLEVAR"
     gene            complement(14211..14903)
                     /locus_tag="VFO53_09170"
     CDS             complement(14211..14903)
                     /locus_tag="VFO53_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HET8876485.1"
                     /translation="MHRLLRAHCAVASTGALRSPLRHLAARLLLLACGIVLATLALAQ
                     GVAPVLLVVGDSISAGYGLARGEGWVDLLARRLAREGYRERIVNASISGDTTAGGRAR
                     LPALLREHQPSIVVIELGGNDALRGGNLDATRANLDAMVKDVLGARAKPLLLGMQVPP
                     NYGAAYARRFASLFTDVAKAHKVPVVPYVFAGFGDDLAQFQDDRIHPKATAEPRIVDN
                     VWPALVPLLARR"
     gene            14969..>15116
                     /locus_tag="VFO53_09175"
     CDS             14969..>15116
                     /locus_tag="VFO53_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8876486.1"
                     /translation="MPSPIIRADRLSKIVQSGDAPLTILDAVSFDVDDGETVAIVGAS
                     GSGKT"
BASE COUNT         2467 a         5120 c         5251 g         2278 t
ORIGIN      
        1 aacatgttga ccacacccgc ggcggcaagc cgataccctc gctcgtggac gagcacggcg
       61 gcgtcgcgtg caagcgtggc cggattgcac gagacgtaga cgatccgctt cagcgcgcgc
      121 gcgtcgtcgc agcccggtag cgacttggcg agcgcgaccg cgccttcccg cggcggatcg
      181 accagcacgc gatcgagcac gccgaggctc gcgatgaagg cgggtgtcgc cgtgaacagg
      241 ttggcgcaga cgaacgtggt cgtcgacgcg agtcggttgc gctcggcgtt ggccgtggcg
      301 cgcgcgatca gcgccttgct gccttcgacg cccgtgacgt ggcgcacgcg acgcgcgatc
      361 ggaagggtga agttgccgag gccgcagaaa aaatccgcga cgcggtccgc cggctgcgga
      421 tcgagcagtt gcacggcgcg gcgcacgagc acgcggttga tggacgcgtt gacctgcgtg
      481 aagtcggtcg gcgcgaacgg tatcgcgacg tcgaactccg gcagggtgta ggcaagactc
      541 gcgtgtgccg gatggaacgg cagcacggta tccgggccgc ccggctgcag ccagaattcc
      601 acgtcgtgcg catcggcgaa cgcgacgagc ttcgcctcgt cggaaacgct gggtggcgcg
      661 agaacgcgca gcaccagcgc atgaacgagc cgctcgtccg tgccgccggt gacctcggcg
      721 agcgcccggg gcgcaagacg ctcgcccacg gcgagctcga tctgaggcag gcgcgtgcgg
      781 atcgacagcg actcgaccag cgtccgcaac gcgggcagca gcgccgagat cttcggcggc
      841 aacacgtggc attcgcgcat gtcggcgaca tagctcgact tgcgctcgtg aaagccgatc
      901 agtacgccgc cctttttcgg cacatcgcgc acggacagcc gcgcacggta gcgatagccc
      961 catgcggggc cggcgatcgc cggcagcatc acgccgggac gcacgcgacc gatgcgggcc
     1021 agcgcgtctt cgagcgcacg ctgcttcgcc gcgagctgca atgcgtcgtc ggcgtgttgc
     1081 agcgtgcatc cgccgcaaac accgaaatgc gggcaacgcg gcggaatgcg ggacgcgctc
     1141 gcaccatgga cggcggccac gcgcgcaatt tcgtagctcg gcttgcgcct gacgacttcg
     1201 gcatcgacgc gctcgccggg cagcgcgcct tcgacgaaca ccaccttgcc gccggcgtgc
     1261 gcgatgcccc ggccctcctg gtccagcgat tcgatcgtga gttcggtcat ggcgagaggc
     1321 gcggattgta cgcgcccgtc cacgcgtgac atcggtaaaa tgggcgcatg aaaatcctcg
     1381 cgctggatac gtccacccga tggctgtcgg tggcgctgtt cgacgggaag cacgcatgca
     1441 cgatccgcga atgcgtcgac accgcggcgt cgcagcgcat cctgccggcc atcgacgaga
     1501 tcctcacccg cgacgggacg ccgctcgcga gcgtcgatgg catcgcgttt ggcgcaggtc
     1561 cgggggcgtt caccggcgtg cgcatcgcct gcgccgttgc gcagggactc gcgttcggcg
     1621 cggaacggcc ggtattcgcc gtgggcacgc tcgaggcgat tgcgcagtcc gcatggcggg
     1681 agcatggctg ggaacgcgtc gtcgcctgcc tcgacgcgcg gatgcacgag gtgtacgtgg
     1741 cggcctacga gcgcgatcgc aacgggtgga tgctcgttcg cgagccggcc gtcgacaagc
     1801 ccggcgcggt ggatccgttc gcgggcggct ggcatggtgc cggagacgcc tttgcggcct
     1861 actccgggct cgcggagcgc ctcggactcg cggcgtacga tggcgcgatc attcccgatg
     1921 cgcagtcggt tgccgaatgc gcttggccgc gggtgcttgc gcacgcaggg gtgcccgcgc
     1981 acgaggcgca gccgctctac gtgcgccacc gcgtcgcgct cacgagcgcc gagcgcgcgg
     2041 ccggcgttcg actctgaaac ggcacgcgtg gcctcgctgc cgaaacccac gccgcttgcc
     2101 cacgtgcgct ggcgtccgct cgccgcgcac gatctgcccg tcgtcgcagc actcgaagcg
     2161 cgcattcatg cggcaccgtg gagcgagcgc aactttcgcg acgcgcttgc cgccggctat
     2221 tcggcgcgcg tgggcatgcg cgccgagcgc atcgtcgcct acggcgtatt gatgctcggg
     2281 cccggcgaag cgcaactgct caatctgtcg gtcgttcccg aggcgcgtcg cggcgggctt
     2341 ggccgcgcgc ttctcctgca gttcatggac gtcgcgaggc agctcggtgc ggagcacctc
     2401 ttcctcgaag tgcgcgtcgg aaacctcggt gccattgcgc tctaccgggc cgagggtttc
     2461 gccgccatcg cgcgccgcgt cgagtactac cctgccgaca agcgcggcgg cgtgcgcgag
     2521 gacgcgctcg tgatgcgctg cgacctgcgc gggtccgccg catcggtcac ctgatgccga
     2581 cacgcaacga ggtgctgcgc gagctgggga ttgcgccgct ctggcggctg cgtgagcgca
     2641 cgcgcacgga tgcgccgcgc ggcgacgagg cggcggacga accgcgcgac gcgaatcgcg
     2701 atcggcgcct cgcgcacatt gcagcgcttg cgctcgacgc gctcgtcgac gacgtcgcgc
     2761 agtgccgtgc ctgcggcctc tgcgccacgc gcaggcgcac ggttccaggc atcggcgccg
     2821 cggacgcggc gtggatgttc gtcggcgaag cgccgggcgc cgacgaggat gcgcagggcg
     2881 agccattcgt cggcgcggcc ggccgcctgc tcgacaacat gctcgcggca ctcgggctcg
     2941 cacgcgaccg cgacgtgttc attgcgaacg tgctgaaatg ccgtcctccc ggcaaccgca
     3001 cgccggcgcc gctcgaagtc gacgcgtgcc gcccgtatct cgaccggcag atcgagctcg
     3061 tcggcccggg gctgatcgtc gcgctcggca agagcgccgc ctgcgcgctg ctcggcgtcg
     3121 acgcgtcggt ggcgagcctt cgcaatcgcg tgcatcgcta tcgcggccac ccgctgatcg
     3181 ttacgtatca tcccgcgtat ctgctgcgca cgccggccga caaggccaag gcgtgggaag
     3241 acttgctgtt cgcgcggcgg acgttgcgcg aatcggtgcc cccgcccgtg tagctgcgcc
     3301 gacgaagttc attggcgaaa aacgcttgcg cacgctggcg cgaggccgca tattccgggc
     3361 ggcaggcaaa tcgtcccacc cttgatcgga ggaggaacat gttgcgcaag ctcggcgtcg
     3421 tgctcgcagc cgtcatcacg atggcgctcg gaggatgcgg ctacaacgac ctgcaacggc
     3481 aggacgaagg cgtcaaggca tcatggtccg aggtgctgaa ccagtaccag cggcgcgccg
     3541 atctcgtgcc gaacctcgtg aacaccgtca agggctatgc gcagcaggag cagcaggtgc
     3601 ttaccgaggt cacgaacgcg cgcgccagcg tcggtgcgat caaggccacg cccgagctca
     3661 tcaacgatcc cgcggcgttt cagaaattca tccaggcgca gaaccagctc cagggtgcgc
     3721 tgtcgcgcct cctcgtggtg tcggaaaact atccgaacct caaatccgac gcggcgtttc
     3781 gtgacctgca agcgcagctc gagggcaccg agaaccgcat cgccgtcgcg cgcaaccgct
     3841 atatcaagac ggtgcaggaa ttcaacagca ccgtgcgcca gtttccgacg aacctgacgg
     3901 cgatgatctt caagatgaac gtcaagccga acttcagcgt tgccgacgaa ggcgcggtca
     3961 gcacgccgcc gaaggtcgat ttcggcaagg gtgcggctcc cgtggtgccc ccaccgggtg
     4021 gcgccacgcc gccttcacct ggaccggcgc cggcgcccgc ccccccgcca gcgccagccc
     4081 cgaagtcctg acgcgatcga tcgtgcgtcc ggccgcgagc gcatcgcggt cggacacgca
     4141 tccacgccga tgatcgcttc cctcgtgcgc atcgattcgt cgccatggcg gcgcggcttc
     4201 gccggcatgg ttgcgctcgt catgctgttc gtcgcgtgcg ccgcggccgg gcagggcaag
     4261 gccgcgtggg aaacggaccg cagcgggctt gcgcccattc cgaagctgac cgcgcgggtc
     4321 accgacgaga ccagcacgct gtcgccggca gagcggcagg cgctggaggc gaagctggcc
     4381 gcctgggagc aaaacagcgg caaccagctg gtcgtgctga tcgtcgcgtc gacgaagccc
     4441 gagccgatcg aatcgtattc gattcgcgtc gccgacgcgt ggaagatcgg gcgcaagggg
     4501 caggacaacg gcgcgatctt cgtcgtcgcg aaggacgatc acaagatgcg catcgaggtc
     4561 ggctacggcc tcgaaggggt cctgaccgac ctgacgtcgc accagatcat cgacgagacg
     4621 gtcgcaccgc tttttcgcca gaacaagttc gccgagggca tcaacgccgg cgtcgaccgg
     4681 atcatcgcgg tggtcggcag cggcgcgccg ctgccggcga aagccgcgac gcagcgtgcg
     4741 cgacagccgt tcgatttcgg cacgctgctg atcttcctct tcgtcggcgt tccgatcgtc
     4801 ggcggcatcc tgtcgcgcat cttcgggcgc atgctcggct cgacgatcgg cggtggtgcc
     4861 gtcggcgcgg ccgcatggct catcgccggg tcgatcgcga tcggcgtggg ggccggtgtc
     4921 gtcgcactcc tgatcatgct gctcttcagt gcgggtggcg gcatcggccg ccggggcggg
     4981 atgtggattc cgaccggcgg gtggggcgga ggcggactcg gcggcggcgg gtttggcggc
     5041 ggcggagggg gtggattctc gggcggtggc ggcagcttcg gcggcggcgg cgcgtcgggc
     5101 ggctggtgac gatgcgccac cgccgcttgt tgcgacactt cgtcaccgat catctgtcgg
     5161 tgaagcgtgc gttcccgccg gcggcgctcg ccctgatcga gcaggcgatc gcagcgggcg
     5221 agcggacgca tcgcggccag gtgtgcttcg ctgtcgaggg cgcgttgccg ccgctgcgcg
     5281 tgctgcgcag gctcacgccg cgcgaacggg cgctcgaggt gtttggcctg cttcgcgtgt
     5341 gggacaccga ggagaacgcc ggcgtgctga tctacctgct gcttgccgat cgcgatgtcg
     5401 agatcgtggc cgaccgcggc atcgaccgcc aggttccgcc cggcgcctgg caggcgatct
     5461 gcgcacggat ggaagcggcg tttcgcgacg cccatctggt cgagggcgtc gtcgtgggca
     5521 tcgaggaaat cggcgcgctg cttgccacgc actttccgcg catcggcgcg gttgcgaacg
     5581 agctgcccga caagccggtc gtcctgtagg gcggcccgcg cagccccctt atgacggctt
     5641 gtcgatgccg atcaggtgca tcgagtcata ttcgcgctga ttcgcgcggt ggcgccacat
     5701 cacgagcagc atcgttcccg tgacgaacat cccgaacagc atgatcgccg tgctgacgga
     5761 aatgcccgcc cgcaccatca gcgcatagag ccccaccatc atcagcacgc cgatgttctc
     5821 gttgaagttc tgcacggcga tcgaatgtcc ggcgcccatc agcacatggc cgcgatgctg
     5881 caaaagcgcg ttcatcggga cgacgaaaaa gccggcgcat gcgccgatcg ccaccatcag
     5941 caccatcgcg agcggcacgt actgaacgaa caccatgacg agcaccacga cgcccattgc
     6001 ggcgccgatc ggcagcacgt tgacggcgcc gcgcagcgag acgaagcgcg cggcgaggat
     6061 ggccccgagc gcgacaccga cggcgacgac cccctgcagc atcgacgcct gcgacaggtt
     6121 gagcgacagc gcgcgcgccg cccagtcgat cacgatgaac tgtagcgttg ccccggcccc
     6181 ccagaacagc gtggtcgtcg cgagggaaat ctggccgagc cggtcacgcc acagcagcga
     6241 cacgcagtgc gagaactcgc ggatcaggta gagcgggttc ttgctcggca cacggtggtc
     6301 gacccccgtg tcggggatgt accagttgaa gatcgcggcg atcaaataga tcgccatgat
     6361 caccgcgatg gcggcgtcgg gcggcgtgtc gatcggcagg tcgatcatcg ggaaatcgaa
     6421 gccgagaagc gcagccgata cgcgcggcgt gatcagcgca ccccccagca cgaagccgaa
     6481 gatgatcgag ccgacggtgg ccccctccat ccatccgttc gcgatgacca gctgctgcgg
     6541 tggcaggagc tcggtgagga tgccgtactt cgccggcgag tacgcggcgg cgccgaagcc
     6601 gaccatcgca tacgacagca gcggatgcac gtcggcgagc atcatcgcgc agccgacgat
     6661 cttgatcgcg ttggtgatca gcatcacgcg cccttttggc atcgaatcgg cgaatgcgcc
     6721 gacgagcggg gcgaggacga cgtacgagac gacgaagctc tgcttcagcg ccggcgtcat
     6781 ccacgccggc tcgtggagct cgcgcagcag cgcgatcgcg gcaatgagca gtgcgttgtc
     6841 ggcgagcgac gagaagaact gcgccgcgat gatcgtgtag aagccgggtt tcatcgagcc
     6901 ttgggagcgg accgcggcct tcgtgatgcg ctggcgtttg ctgtcggttg ctataatgcg
     6961 tccatgcgcg gccggcgctt tataccatga tcgcacacgc tgtcccaccg ctgccgcatc
     7021 cgccgcgcgc gccgttcccg gcctgacgcc cgcgtctgtc gtcgattccg cgttcgcctt
     7081 ctgcgcatgg cccgaccgct cgttgcgcac atcgataccg gcgcgctcgc ggccaatctc
     7141 gcctgcgccc gcgcgcttgc ggccggtgcc agcgtgctgg ccgtcgtcaa ggccgacgcc
     7201 tacggtcacg gactcgcccg cgtgctaccc gcgctcaccg cggccgacgg gcttgcgctg
     7261 gtcgagctcg acgccgccgt gcgcctgcgc gagcatggcg agcggcgccc gatcctgctg
     7321 ctcgagggtt ttttcgagcc cgccgagctc gatgcattgg ccgcacattc gctggccacg
     7381 gtcgtgcatt gcgccgatca ggtcgacctg ctcgagaagg cgcgcgtgtc gcggccactc
     7441 gacgtctacc tcaaggtcaa taccggcatg aaccgcctgg gcatcgcgcc cgccgatgtg
     7501 cgcgcgctgg cgcagcggct gtcgtctgcg cgcagcgtcg cgtcgttgca cctgatgacg
     7561 catttcgcgc gtgccgacga acccgacgcg atcgccgagc cgctcgcgcg cttcgccgcg
     7621 gcgtgcgcgg gcctgcctta cccgcgctcg ctggccaatt cggcgggcgt cgtccgctac
     7681 gccgacgtgg gcggcgacgt cgtgcgtccc ggcgtcatgc tctacggcgc aacgccgttc
     7741 gcggcgcgca gcgcgcaggc gctcgggctt tcgccggtca tgacgctgcg ctcgaagctg
     7801 atcggcgtgc agacgctcgc agcgggcgaa agcgtcggct acgcggccac gttcacggca
     7861 gcgcgcccaa tgcgcgtcgg tatcgttgcc tgcggttatg ccgacggcta tccgcgccac
     7921 gcgcccaacg gcacgccggt gctcgtcgac ggacggcgcg tgccgatggc cggacgcgtg
     7981 tcgatggaca tgctgacggt cgacctttcg agcgtgccgc aagccggcat cggcagcgac
     8041 gtcgtgctgt ggggcgaagg cttgccggtc gacgacgtcg ccaaggcggc cggcacggtc
     8101 ggctacgagc ttctgtgcgc cgtcgcgccg cgcgtgcgtt tcgaagtcca cgagccggca
     8161 ggcgcggcct gaaggccgcg ttcgcgcgag gagaagcgtc ttgtacgtcg tcgtgaccgg
     8221 cgcggccgga ttcatcggat ccaatctcgt gcagggcctg aacgcgcgcg gcgagacgga
     8281 catcatcgcg gtcgacgatc tgacgcacgg cgacaagttc aggaacctgg tcgactgccg
     8341 catcgccgat tacatcgaca aggacgagtt cctcgccggc atcgccgacg gtgatttcga
     8401 cgacgatatc gcggtgatct tccaccaggg cgcctgctcg gacaccacgg ccagcgacgg
     8461 gcggtacgtg atgcgcaaca actaccggta ctccgttgcg ctgctcgagc actgccagga
     8521 caacgacatc ccgttcctgt atgcatcgag cgccgcggtg tacggatcgg gcagcgtgtt
     8581 ccgcgaagag cgtgcgcacg aggcgccgct caatgtgtac gggtattcga aatcgctgtt
     8641 cgacgattac gtgcgccgcg tgctgcccga gcgcacggcg ccgatcgccg gctttcgcta
     8701 tttcaacgtc tacggtccgc gcgaggcgca caagggccgg atggcgtcgg tggcattcca
     8761 ctttttcgga cagtaccggc gcgacgggcg cgtgcagctg ttcgaaggtt ccgaagggta
     8821 cggcaacggc gagcagcggc gcgatttcat ccacgtcgac gacgtcgtcg cggcgaatct
     8881 gcatttctac gggcgccccg agcgcgcggg catcttcaat ctcggcaccg gcgaagccgc
     8941 gacgttcaat gcggtcgcgc atgcgacggt caacgcctgc cgcgccgcgg cgcaagacgc
     9001 gccgcgttcg ctcgagcaac tcgttgccga cggcatcatc acctacgtgc cgatgcccga
     9061 ggtgttgcgc ggcaagtatc agagcttcac gcaggcggat gtcacgcggc ttcgcgcggc
     9121 agggtatgtg gcgccgatgc tgaccgtcgc ggaaggcgtg ccgcgctacg tcgaaagtct
     9181 gatttcggcc gccggacctc gttcgtaagc tgggtgctcg atcgggttgc gccgcgtcat
     9241 gcgcgcgcag cacgacgatt cctcccatta cctcgcgaag gagtgttcca tgatccgtct
     9301 cttgcttgcc ctcgtcgtcg cgctcgcaac gccgctcgcc gacgccgcac tcaacctcaa
     9361 caccgccacg aaggacgagc tcgtcgcgct gtccggcatc ggtccggcga aagcgcaggc
     9421 cattctcgat taccgggtcc agcatggcgg tttcaagtcg gtcgatgagc tgaaggacgt
     9481 caagggcatc ggtgcgcgcc ggttcgaaaa gctgaagccc gagctcaccg tcgtgccggc
     9541 gccggtgaaa acggcggcac gcgaggcgaa gcccgcggcg gccgccaaat ccgaggcgaa
     9601 gtcgaacaag tgacgtgtgg ggctgcaccg gcctggcggc aaccggccgg tgcggcccgc
     9661 gcgctctcgg gcgctgcgca ggcggaccgt ggtcaacgcc gtgctgccgt tgcgcgttac
     9721 tggcgcgcgc cgagctcccc gcgtcgcggg cacgaaggcc ggcgttggtg cggcattccg
     9781 catgcgtcag ggagaacgac atgaaacgtc tgttgctggc tttcgtactg cttttcgctg
     9841 cgagcttcgc attcgcggcg gtcaacctca acacggccac gaaggaagag ctcgacgcat
     9901 tgccgggcat cggccccgtc aaggcacagg caatcatcga ctatcgcaac gcgaacggtc
     9961 cgttccggac acccgaggat gtcatgaagg ttcgcggcat caaggaaggc gagttcggca
    10021 agatccgcga ccagatctcc gtgtccggca gcacgacccc ggtgcagcgg ccggcggccg
    10081 cgacgaccgc cccggcaacg gcagcgccgc gcgccgccgc gccggccccg gccacgcctg
    10141 ctgcggcgcc cgccccggcc cggaccgcgg cgccggcatc cacggcaacg gcgccagcgt
    10201 cggccgcgcc cgccaaggcc gacaacgcaa agatgacgaa ggccgaggaa aaggcggccc
    10261 gggcgaaggc acgcgaagaa aaggccgcca gggccaagga agaaaaagcg gccaaggcga
    10321 aggagaagca ggagaaggcc gcgaaggcga aggaagacaa gacagccaag gccgaggcga
    10381 cgaagaccaa ggcatcggcg aaggacaagg ccgccgccga gacgtccgcg aaggacgtga
    10441 agcccaagcc ataatgctgt cgtagccaaa cggtgccggc cggcagcatg tccggccggc
    10501 gccgtcatgc aatgggcgtc gcgcggcagt ccgctacaat tcgcgcatga gctatgcaac
    10561 gctcgccgac gccgtcggca acacgccgct cgtcaggctg atgcggctgc ccggcgcgac
    10621 gtcgaacgtc atcctcgcca agctcgaggg caacaacccg gcgggatcgg tcaaggatcg
    10681 ccccgcgctg tcgatgatcg tcgaggccga gaagcgcgga accatcaagc ccggcgacat
    10741 gctgatcgaa gcaacgtcgg ggaacaccgg catcgcgctt gccatggtcg ccgcaatgcg
    10801 cggttaccgg atgctgctcg tgatgcccga aagccagacg gccgagcgtg tgcagacgat
    10861 gcgcgcgtac ggcgccgagc tcgtgctcac gccgaaggcg ggtgggatgg aggccgcgcg
    10921 cgatatcgcg atgcgcatgc gcgacgaagg caggggcgtc atcctcgacc agttcgcgaa
    10981 ccccgacaat ccgctcgcgc attaccgcgg cacggggccc gagatctggc gcgacaccgc
    11041 gggccgggtc acgcatttcg tttcggcgat gggcaccacc ggcacgatca tgggcgtgtc
    11101 gcgcttcctc aaggaacaga atccggcgat cacgatcatc ggtgcgcagc ccgaggaggg
    11161 ctcgtcgatc ccgggcattc gcaagtgggc gcccgcgtac ctgccgaaga tcttcgatgc
    11221 aagccgcgtc gatcgcaccg agaacgtgtc gcaggccgat gccgaggacc acgcgcggcg
    11281 gctcgcgcgc gacgaaggca tcttctgcgg catcagctcg ggcggcgcgt gtgctgtcgc
    11341 gctgcgcctg tcgcaaggca tcgaaaacgc gacgatcgtc ttcatcgtct gcgatcgcgg
    11401 cgaccggtac ctgaccaccg gcgtatttcc cgcctgatgc gcattgcgtc ccgccagcgg
    11461 cgcagtttgc gcgtcgctgc ctgaacgggg cgggtcccga tgattcccct gctcgtcttc
    11521 gacatcgaga ccgtgcccga catcgcgggt atccggcgta tccacgaaat cggcgatgcc
    11581 gtcgatgacg cgggcgtcct cgactggttt ctgcagcgcc ggcgcgcggc gagcggcagc
    11641 gacttcgcgc cgctgcatct gcagaaggtc gtcgccatcg cctgcgcgat gcgcgatacc
    11701 gccggcattc gcatctggtc gatcggcgat ccggccgacg acgagcccga gctcatccgg
    11761 cgctttttcg acggcatcga gaaatacacg ccgcagctcg tgtcgtggaa cggcggcgga
    11821 ttcgacctgc cggtgctgaa ccaccgtgca ctgatccatg gcatcgcggc gtcgcgctac
    11881 tgggactggg gcgacgacga caaggagttc aggtacaaca actacctcgg gcgctatcac
    11941 acgcgccatc tcgacctgat ggacgtgatg gcgcaatacc agcttcgcgc cagcgccggg
    12001 ctcgacgcga tggcgcggct gtgcggcttt ccggggaagc tcggcatgga cggcagcgaa
    12061 gtcgccgcgg cgatcgccgc cggacgcatt gccgacgtgc gcagctactg cgagtgcgac
    12121 gtgctcaaca cgtatctcgt ctaccaacgc ttccggctga tgcgcggcga aatggacgcc
    12181 ggcgagtacg cgcgcgaagt gtcgctcgtg cgcgagcgca tcggcgcgtc cgacgccgcg
    12241 cactggcgcg cgttcatcgc gcagtgggac ggcgcatcgg tcacagcgtg atcgagccgc
    12301 ggccgatctc gatcacgcgg cccgacacat ggatcgagca ctgcgaatcg acgaagagcc
    12361 cgaggcggca cggccttccg accgcttcac cctgatcgat cgtcagccgc tgcggcagcc
    12421 tggcgccggt tgccagcagc cagccgccga ggttcgcgca tgccgagccg gtgcctggat
    12481 cctcgatcat cgcgccgtgc ttgggaaaga agaagcggga cacgacgtga tcgccgtcgc
    12541 gcgcgaaaac atacgccatc gcgcgttgcc cgttgcttgc gtgcaagagc atttgcgcag
    12601 gcgacggccg cgcgcggcgc acggcgtcga acgacgccag cggcacgatg aattgctcgc
    12661 tgccggtgtc gacccacagc ggatcggccg cggccagatc gtcgatcgac aagccgagca
    12721 ttgcggcaag ctcgctgcgc gacgccgatg acgcgcgatg ccgcggcgcg ttcgccgtca
    12781 gcgtccagac gtcgccgtcg gcatgcacgg gaatgacgcc ggcgtgcatc tgcagcgtca
    12841 cctggtcgcc ggcgtccgcg agcgtgcgga cgacgtgcgc ggtgccgagc gtcgggtgac
    12901 cggcgaacgg catctcgaat gtcggcgtga agatccgcac gcgccgtgtc gcggcctgcg
    12961 atggcagcac gaacgtcgtt tccgacagat tgaactgcag cgcgagcgcc tgcatcgtcg
    13021 cgtcgtcgag cccgcgcgca tcctcgaaca cggcgagcgg attgccggcg agcggcgttt
    13081 cggcgaacac gttgacgatg cgaaacgcgt agtcggtcat cgcgcaacct cgagcggcaa
    13141 ggccccgagc agctgccgcg cgaggccccg gaacgtctcg tcccgcgcgt cgtcgacatg
    13201 caggaccacg gcgttccggt agtgcgggaa attgcgcgcg atcgcgcgct cgtaggaggg
    13261 atcatagtgc acgtcgagaa gctcggtggc gagctcgcgc acggcacccg attccatcat
    13321 cgcctgccag cgcgcgagca cggcgttgcc atgcaggctc gcgagcagcg caagctgcgc
    13381 cgtgaatgcg acgggatcgc tcgccaggtg cgcgtaatcg gacgcgagca tgacaacacg
    13441 gttcgacgtc gacgtttcga gccggatgca gggtgcctcg cgcatcgact cgagcaacgc
    13501 gtccggaacc tgcaggcgtc cgatgcgccg gctttccgat tcgacgaaca ccggacgcga
    13561 ggcgtcgaag cgcgcgaacg attcgagcag cgcgctttcg aacgccttct gcgtcggctg
    13621 cggcgcgccc ggaatgtcac cgaggagcga gccgcgatgg cacgcgagcc gctcgagatc
    13681 gagcacctgt ccgccttcgg ccgcgatcgc ctcgatcagg cgcgatttgc ccgagccggt
    13741 gaatccgcaa acgacgcggt agcggaactg tcgcggccat tgcgcgagca gcgcgacgac
    13801 gtgacgccgg taggcgcggt agccgccgtc gagctgcacg gcgcgaaagc cgatctcgtt
    13861 cagcacgtgc acgagcgcgc ggctgcgctg cccgccgcgc cagcagtaca cgagcggcga
    13921 ccagtcgcgc ggcttgtcct gcacgtgcgt ggcgacgatc gccgcgatgt tgcgcgcaac
    13981 gattgccgca cccgccttgc gcgcatcgaa cgccgatacc tgaacgtagg tcgtccccac
    14041 gcgcgcgcgc tcctcgtcgt cgagcaccgg caggttgatc gcgcccggca gatgatcctc
    14101 ggcgaactcg ccgggcgagc ggacgtcgat gcgatcgcga aacgaggcga aatcgtcgac
    14161 gctcgcgcgg gtctcggcgc gtagcggcgg accgggcggc agcggggcga tcatcgtcgc
    14221 gccagcagcg ggacgagcgc gggccagacg ttgtcgacga tccgcggttc ggcggtggcc
    14281 ttcgggtgaa tgcgatcgtc ctggaactgc gccaggtcgt cgccgaagcc cgcgaacacg
    14341 tacggcacga cgggcacctt gtgcgccttc gccacgtcgg tgaacagcga cgcgaagcgc
    14401 cgcgcatacg cggcgccgta attcggcggc acctgcatgc cgagcagcag cggcttggcg
    14461 cgggcgccga gcacgtcctt caccatcgcg tcgaggttcg cgcgcgtggc gtcgagattg
    14521 ccgccgcgaa gcgcgtcgtt gccgccgagc tcgatgacga cgatcgatgg ctggtgctcg
    14581 cgcaacagtg ccgggaggcg cgcgcgtcct ccggccgtcg tgtcgcccga aatcgacgcg
    14641 ttgacgatac gctcgcggta gccttccctg gcgagccgcc gcgcgaggag gtccacccag
    14701 ccttcgcctc gcgcaagccc gtacccggcg gagatcgaat cgccgacgac gagcagcacg
    14761 ggtgcgacgc cctgcgcgag cgccagtgtc gcgaggacga tgccgcaggc gagcaacagc
    14821 aggcgcgcgg cgaggtggcg gagcggcgat cgaagcgcgc cggtcgacgc gacggcgcaa
    14881 tgtgcgcgaa gcaggcgatg catcggcaaa ttgtaaccgg ccgctgttca ccggccgcac
    14941 gaaacgtgcc gcgtagaatc caaggcggat gccctctccg atcattcgcg ccgaccgtct
    15001 ttcgaagatt gtccagagtg gcgacgcgcc gctcacgatt ctcgacgccg tgagcttcga
    15061 cgtcgatgac ggcgaaacgg tcgccatcgt cggtgccagc ggctcgggca agacga
//
LOCUS       DASSVX010000213        11855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1108700, whole genome shotgun sequence.
ACCESSION   DASSVX010000213 DASSVX010000000
VERSION     DASSVX010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 11855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11855
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108700"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /gene="acnA"
                     /locus_tag="VFO53_09180"
     CDS             <1..439
                     /gene="acnA"
                     /locus_tag="VFO53_09180"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264018.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET8876487.1"
                     /translation="IYDAAMKYIADGTPTAVFGGEEYGTGSSRDWAAKGTQLLGVKAV
                     IAKSFERIHRSNLVGMGVLPLQFVAGDSVESLGINGDETFDIAVPRDIRPQQRFTVAM
                     TRRDGTTREIPVLCRIDTPIEVDYYHHGGILPFVLRELLQPAA"
     gene            436..1311
                     /locus_tag="VFO53_09185"
     CDS             436..1311
                     /locus_tag="VFO53_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8876488.1"
                     /translation="MTRPGGAHPLQYALPLFVVAGFCLSSLDATAKYLVRDHSLLVVV
                     WARYAGQMVVVTPYAWHRAGRGFWRTRRPGMQLLRSLFLLLATLGFWGGLRYLPLAEA
                     SSVTFLAPTFVVLLSWPLLRERATHARRLASTVGFIGILILLRPGSAVMHPAVLLLIG
                     AALSNALYQLLTRKLLDESIHATLFYSASVGTLALTLGLPFANDLDHLVAVGVAPLVL
                     LGMLAGIGHWCMTQAYLRAPASLLSPFTYLQILWATAFGYLMFDQHPDAVSALGMAVV
                     VASGVGLAAWERGRR"
     gene            1484..1885
                     /locus_tag="VFO53_09190"
     CDS             1484..1885
                     /locus_tag="VFO53_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876489.1"
                     /translation="MKTRMLATLIAMALAATLSACGQQSQQAAEKAKESAGAAADSAA
                     TAAKDAATAASAAAKDATNAAADAAKGAADATKEAADKAAEATKDAGTKAADATKDAA
                     AQAADATKDAAAKASDAAAKANEAAKDAAKK"
     gene            2122..3201
                     /locus_tag="VFO53_09195"
     CDS             2122..3201
                     /locus_tag="VFO53_09195"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET8876490.1"
                     /translation="MQSSTVLTRTEAGRAELAVPAHGLSLAQRRFLTLLDTSRTVDEL
                     AQRHRAEAAKLDRDLSRLAELGLVVCDIGVPANDAEAAPPAAVRLGATSGSRLPWMLL
                     LLAVAGGLAFAGWRQWRAADSAAADRNAHAPASRKATPDVQAAPVADPAPIATRVLKG
                     DPAERTRDAAKESRGQPKSAERRPENPPAKPVDLPVEHRSPPHDDAAHASAPLVPGPV
                     PPLQLAPIPVAAANPVPLSTPLLTPAIPGPAANVTAAMPPIQVATAAPASGLLRPAPL
                     PALVPIAREPPSFPREAIALGLADGTVRARLTIDAKGNVSSVDIVEASHRAFTRTVRE
                     TLMRWRFEPGTAGRTTSVDVAFKRD"
     gene            3235..4116
                     /gene="ttcA"
                     /locus_tag="VFO53_09200"
     CDS             3235..4116
                     /gene="ttcA"
                     /locus_tag="VFO53_09200"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HET8876491.1"
                     /translation="MQESLVTLVPPAVPARARDPRKAEFETGKLKKRLRRLVGQAIAD
                     YAMIGAGDRVMACVSGGKDSYGMLDVLLSLQKRSPQPFTIVAVNLDQKQPGFPADVLP
                     RYLAGRGVEFRIVEQDTYSVVKRLIPDGATMCSLCSRLRRGVLYRVAREVGATKIALG
                     HHRDDILATFFLNLFHGGKLKTMPPKLVSDDGMHVVIRPLAYVRERDLARYAEACAFP
                     LIPCTLCGSQENLQRKHVAAMLREWEKRFPGRIESISNAMASVVPSHLLDRTLHDFAG
                     VRASGVASADGDRAFDP"
     gene            complement(4118..4816)
                     /locus_tag="VFO53_09205"
     CDS             complement(4118..4816)
                     /locus_tag="VFO53_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET8876492.1"
                     /translation="MIDLYYWTTPNGHKVTMFLEETGLAYTVKPVNISRGEQFAPAFL
                     AVAPNNRIPAIIDHAPAIGGAAPLPLFESGAILLYLAEKTGRLIPADARERWQCIQWL
                     FWQMAGLGPMAGQNHHFGGQSVEPIPYAIDRYVNETGRLYAVLDRQLRGREFIVDGYS
                     IADIACYPWIQPQRQRQNLDDFPELKRWKAAIAARPATQRAYAIAKTVNVQPAVSDEE
                     SRRILYGQGKHTVR"
     gene            complement(4840..5619)
                     /locus_tag="VFO53_09210"
     CDS             complement(4840..5619)
                     /locus_tag="VFO53_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8876493.1"
                     /translation="MDLGIAGRRALVCAASKGLGRGCAEALAREGVDVTICARTEADV
                     ARTADEIGALAGRKVAWVACDITTPEGRAKVLGACPQPDILVNNAGGPPPGDFRDWDR
                     AAWIRALDANMLTPIELIKATVDTMIERKFGRIVNITSSAVKAPIDILGLSNGARAGL
                     TGFVAGIARRVVRHNVTINNLLPGQFATDRLRTTMEGRAKASGKAYDEVRDAAMNATP
                     AGRFGDPREFGAICAFLCSTHAGYIAGQNILADGGAYPGTF"
     gene            5785..6183
                     /gene="msrB"
                     /locus_tag="VFO53_09215"
     CDS             5785..6183
                     /gene="msrB"
                     /locus_tag="VFO53_09215"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET8876494.1"
                     /translation="MNDTTRKTDSEWQAQLTPEQYRVTRQKGTERPFTGEYWDNEAEG
                     TYRCVCCGTPLFESDTKFDAGCGWPSFNAPIASANVQERSDDSYDMRRTEVVCAACDA
                     HLGHVFSDGPQPTGLRYCMNSASLEFEPKK"
     gene            complement(6242..6955)
                     /locus_tag="VFO53_09220"
     CDS             complement(6242..6955)
                     /locus_tag="VFO53_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET8876495.1"
                     /translation="MTDELLDAIEIETAPNPDAAVIWMHGLGDDGRGWSEVVPSLGLP
                     KHLAIRFLFPHAPIMPVTVNNGMRMRAWYDIRQANVSERADVDGVRRSQSHVGALLAR
                     EASRGIVASRTLLAGFSQGGAIALFAGLRFDARLAGVIALSAYLIAPERLAAEASAAN
                     RDVPIFMAHGTQDQVIAFAWAEHSRDVLQAGGWPLEWHAYPMDHAAGVEEIVAAGAFI
                     ARVLTDRRSFPRQPVSETS"
     gene            complement(7023..7994)
                     /locus_tag="VFO53_09225"
     CDS             complement(7023..7994)
                     /locus_tag="VFO53_09225"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET8876496.1"
                     /translation="MRLRTFVFVMLACLGSMAHPAQAAWPERPIKVIVPFPAGGQLDV
                     VVRLIAEKISPALGQPIVVEVHTGADGNIGAELAARSAPDGYTWFATSVPFTTQAALQ
                     PGKLRYDPLRDFLPVANLGTASFVLVVPNSVPVTTLREFIAYAKARPGELSYAGTSVG
                     SVTHLSTEMFARAVGIRMEMIPYAGIPAAMSDLLAGRTQFMSTGVVAALPQIKAGKLK
                     PLAILAPERHPQLPNVPTIVEEGHPDLTVATWFGLLVPAKTPADVVQRINAEIMKAMR
                     DPEVLARYRTMGVDPVAPHPPEAFGRLLQSEIDRWGNVIRDAHVTVQ"
     gene            complement(8033..10354)
                     /locus_tag="VFO53_09230"
     CDS             complement(8033..10354)
                     /locus_tag="VFO53_09230"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET8876497.1"
                     /translation="MNTAPPRESVDRLRCGDPAAGYEGTIGRTLAESSPWWPAAQRAP
                     DRAPNVVVILLDDLGFSDFGCYGAEIRTPHIDALAAGGLRFSNYTTVPMCTPARAALL
                     TGRNPHAVGCGWLTFNLPGYPGYQAGEIARDAPTLAELLRANGYSTYAAGKWHNTADY
                     NVLPSAARGSWPVQRGFDRFYGFLGGETHYFAPAQLVCDNAFVDCDRYRDGYYCSDDF
                     TDTAIGWLKAHASASPDKPFFLYLPYNAPHAPLHAKTEDLARYAHLYDEGWDRVRSAR
                     VARQRALRLHAREWRLPSRSGGVPAWDSLDAEARRLHARFMALYAAVVDNVDRNIGRV
                     VACLSALGKLDDTLVVVTSDNGANGIGGPDGAVNNLAKRLVQREDPAWVRTMLESGGI
                     GGAASWPAYSLGWTDVSTAPFRLYKTTTMNGGIRVPLVAHWPRGIADRGAIRHQFVHV
                     TDIVPTILDVLGAGYPESFAGYRTRALDGASFRPVLADAAAPAARTRQHYELAGNRGY
                     IEDDWKIVSLQPPGTPIDLHNWMLFDLGADATETQDLAAGQPDRLRDLVAAFDADARA
                     NHVYPLDNRGVRRSLTVPPFLEASVNAPRTFYPGCGTAALAAVAPLVADRDYRLECGF
                     THDGSDEGVIFALGDPIAGMALFARAGRVAFVYHGGEGRPVVCDGLRVHRGDNRFVLD
                     HRAGGERRGQGALFVNGEAAGTLDMSPTTILGLGVGEGLDVGCDRKLHVTPLYGRDDA
                     FAYTGVVSYVRIEPGAQAHDSYANRPERLAQRD"
     gene            complement(10393..11397)
                     /locus_tag="VFO53_09235"
                     /pseudo
     CDS             complement(10393..11397)
                     /locus_tag="VFO53_09235"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
     gene            complement(11394..>11855)
                     /locus_tag="VFO53_09240"
     CDS             complement(11394..>11855)
                     /locus_tag="VFO53_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8876498.1"
                     /translation="IAGIAWLPLTIIWFGMGNGSVIFVIANTIFFSALYNTVIGVQSI
                     PLALHRAVRSHGGRGLALLANLILPGAMVQILLGIRTSVAYGWRALVASEMLAGSNGL
                     GYMTMEAVQWQRTEKIILGMIVIGLLWVLMDRVVFTPIERRTVGRWGLLQR"
BASE COUNT         1868 a         4137 c         3993 g         1857 t
ORIGIN      
        1 gatctacgac gccgccatga aatacatcgc cgacggcacg ccgaccgcgg tgttcggtgg
       61 cgaggaatat ggcaccggct cctcgcgcga ctgggccgcg aaaggcacgc agctcctcgg
      121 cgtcaaggcc gtgattgcga agagcttcga gcgcatccat cgctcgaatc tcgtcggcat
      181 gggcgtgctg ccgctgcagt tcgtggcggg cgacagcgtc gaatcgctcg gcatcaacgg
      241 cgacgagacg ttcgacatcg cggtgccgcg cgacatccgc ccgcagcagc ggttcaccgt
      301 cgcgatgacg cgccgcgacg gcaccacgcg cgaaattccc gtgctgtgcc gcatcgacac
      361 gccgatcgag gtcgactact accaccacgg cggcatcctg ccgttcgtgc tgcgcgaatt
      421 gctgcagccg gcggcttgac gcggcccggc ggcgcgcacc cgctgcagta cgcgctgccg
      481 ctgttcgtcg tcgccggttt ctgcctgtcg tcgctcgacg caacggcgaa atacctcgtc
      541 cgcgaccatt cgctgctcgt ggtcgtctgg gcgcgctacg ccggacagat ggtcgtcgtc
      601 acgccgtacg cgtggcatcg cgccgggcgt ggcttctggc gcacgcgcag gcccgggatg
      661 cagcttctgc gctcgctctt cctgctgctc gcaacactcg gcttctgggg cgggctgcgc
      721 tacctgccac tcgcggaagc ctcgtcggtg acgttcctcg cgccgacatt cgtcgtgctg
      781 ctgtcgtggc cgctgttgcg cgagcgtgcg acgcatgcgc gccggctcgc ctcgaccgtc
      841 ggcttcatcg gcatcctgat cctgctccgt ccgggatcgg cggtcatgca tcccgcggtg
      901 ctgctgctta tcggcgcggc gctgtcgaac gcgctctacc agctgctgac gcgcaagctt
      961 ctcgacgaat cgatccatgc gacgctcttc tacagcgcga gcgtcggcac gctcgcgctg
     1021 acgctcggcc tgccgtttgc aaacgacctc gatcatctcg tcgccgtcgg tgtcgcaccg
     1081 ctcgtgctgc tcggcatgct ggcgggcatc gggcactggt gcatgacgca ggcgtacctt
     1141 cgcgccccgg cctcgctact gagcccgttc acgtacctgc agatcctctg ggccacggcg
     1201 ttcggctacc tgatgttcga tcagcatccc gacgcggtgt cggcgctcgg catggccgtc
     1261 gtggttgcga gcggcgtcgg cctcgcggcc tgggagcgcg ggcgccgttg aacggctgtt
     1321 gtaattgcgc cgcagcgcgg caccgcacga gggaaaccgt tgtctttttg tgcagtgcaa
     1381 cgagccgcgg gttaaaatga gcggctgcgg aaaccggtct ttcgacgaag ggtcttccgc
     1441 ccactctttt cgacgttcca accttgaata ccgggagtaa tcgatgaaaa ccagaatgct
     1501 cgccacgctg atcgcgatgg cgctcgccgc cacgctcagt gcatgcggac agcagtcgca
     1561 gcaggccgct gaaaaggcga aggaatcggc cggtgccgcg gcggattcgg ccgccaccgc
     1621 cgccaaggat gccgccaccg ccgcctcggc ggccgcgaag gacgcgacga acgccgctgc
     1681 cgacgctgcc aagggtgccg ccgacgcgac gaaggaagcc gccgacaagg ccgccgaggc
     1741 aaccaaggat gcaggcacca aggcggccga cgcgaccaag gatgccgccg cccaggcggc
     1801 cgatgccacg aaggacgcag cggccaaggc aagcgacgcg gcggcgaagg cgaacgaagc
     1861 cgcaaaggac gcggcgaaga agtaacgcgc ccgcatcctg cagcagcgtg acggccgtcg
     1921 cagcgacggc cgttgtgctt tttgcggcgg catgcgcggc gacgcccgcg cgttcgtcgg
     1981 cgcgcggctg ccatggcatt actgttgcac cgcgcttacg tggttgcgcc gtcgcgcgct
     2041 ttcccgtcca aagggcgcgg tgactcgttc gccacgcagc gcgctcgccg caaccacccc
     2101 gatttcctcg agggagcgag cgtgcagtcg tctaccgtcc tcacgcgaac cgaagcgggc
     2161 cgtgcggagc tcgccgtgcc ggcgcacggc ctgtcgctcg ctcagcgccg ctttctgacg
     2221 ctgctcgaca cttcccgcac cgtcgacgaa ctggcgcagc gccatcgcgc cgaagccgcg
     2281 aagctcgatc gcgatctttc acgcctggcg gagctcggcc tcgttgtatg cgacatcggg
     2341 gttcccgcca acgatgccga ggcggcaccg ccggccgccg tccggctcgg cgcaacatcc
     2401 ggaagccgcc tgccgtggat gctgcttttg ctcgccgtcg caggcgggct cgcgttcgcc
     2461 gggtggcgtc aatggcgggc tgccgactcc gcggcggccg accgcaacgc tcacgcaccg
     2521 gcgtcgcgca aggcaacgcc tgacgtgcag gctgcgccgg tcgccgaccc ggcaccgatc
     2581 gcaacgcgcg tgctgaaggg cgatcctgcc gaacgcacgc gcgacgcggc gaaggagtcg
     2641 cgcggtcagc cgaaaagcgc cgaacgccga cccgaaaatc cgccggcgaa gcccgtcgac
     2701 ctgccggtcg agcatcgaag cccgccgcac gacgacgccg cacacgcgag cgcaccactc
     2761 gttccgggcc ccgtgccgcc gctgcagctt gcgccgattc ccgttgcggc ggcgaacccg
     2821 gtaccgctgt ccacacccct gctgacgccg gccatcccgg gacccgcggc gaacgtgacc
     2881 gcggcgatgc cgccgatcca ggttgccacc gccgcgccgg cgagcggcct gctgcgaccg
     2941 gcacctctgc ccgcgctggt gccgattgcg cgcgaaccgc cttcctttcc gcgcgaggcg
     3001 atcgcgctcg gcctcgccga cggcactgtc agggcacgtc tcaccatcga cgccaagggc
     3061 aacgtcagca gcgtcgacat cgtggaagct tcgcatcgcg cgttcacgcg cacggtgcgc
     3121 gagacgctga tgcgctggcg cttcgagccg ggcacggcgg gacgcacgac cagcgtagac
     3181 gtcgcgttca agcgcgactg acgcggcggc acccagccgc cgtaaaatcg gcggatgcag
     3241 gagtccctcg tcaccctcgt tccgcccgcg gtgcctgcgc gggcccgcga cccgcgcaag
     3301 gccgaattcg agaccggcaa gctgaagaag cgcctgcgcc gtctcgtcgg ccaggcgatc
     3361 gccgactacg cgatgatcgg tgcaggcgac cgcgtgatgg cgtgcgtgtc gggcggcaag
     3421 gacagttacg gcatgctcga cgtcctgctg tcgttgcaga agcgctcgcc gcaaccgttc
     3481 acgatcgtcg ccgtcaatct cgaccagaag cagccgggct ttcccgccga cgtgctgccc
     3541 cgctacctcg ccggccgcgg cgtcgaattc cgcatcgtcg agcaggatac gtacagcgtg
     3601 gtcaagcggc tgattcccga cggcgcgacg atgtgctcgc tgtgctcgcg gctgcgccgc
     3661 ggcgtgctct atcgcgtcgc acgcgaggtg ggcgcgacga agatcgcgct cggccaccat
     3721 cgcgacgaca tcctcgccac gttcttcctg aatctctttc atggcggcaa gctgaagacg
     3781 atgccgccga agctcgtctc cgacgacggc atgcacgtcg tcatccggcc gcttgcctac
     3841 gtgcgcgagc gggacctcgc gcgctatgcc gaggcctgcg cgttcccgct gattccatgc
     3901 acgctgtgcg ggtcgcagga gaacctgcag cggaagcacg tcgccgcgat gctgcgcgag
     3961 tgggagaagc ggttcccggg acgcatcgag tcgatctcca acgcgatggc cagcgtcgtg
     4021 ccgagccacc tgctcgaccg gacgctgcac gattttgccg gcgtccgcgc gagcggcgtc
     4081 gcgtcggcgg acggcgatcg cgcgttcgac ccctgaatca cctcacggtg tgtttcccct
     4141 gcccgtagag gatcctgcgt gattcctcgt cgctcaccgc gggctgcacg ttgacggttt
     4201 tcgcgatcgc gtaggcgcgc tgcgtggccg gacgggcggc gattgccgcc ttccagcgct
     4261 tcagttccgg aaagtcgtcg aggttctgcc gctggcgctg gggctgtatc cacggatagc
     4321 aggcgatgtc cgcgatcgaa tagccatcga cgatgaactc gcgcccgcgc aactgccggt
     4381 cgagcaccgc gtagagccgc cccgtttcgt tgacgtagcg gtcgatcgcg taagggatgg
     4441 gctcgaccga ctggccgccg aagtgatggt tctgtcccgc catcgggccg agacccgcca
     4501 tctgccagaa aagccactgg atgcattgcc agcgctcgcg cgcatcggcg ggaatgagcc
     4561 gtcccgtttt ctcggcaagg tagagcagga tcgcgcccga ctcgaacagc ggcagcggcg
     4621 ccgcgccgcc gatcgccggc gcatgatcga tgatcgccgg aatgcggttg ttcggtgcga
     4681 cggcgaggaa cgcgggcgcg aactgctcgc cgcgcgagat gttgaccggc ttcaccgtgt
     4741 aggcgagtcc cgtctcctcg aggaacatcg tgaccttgtg gccgttgggt gtcgtccagt
     4801 agtagaggtc gatcattggc agggttcagc tagcacgcat cagaacgttc ccggatacgc
     4861 gccgccgtcg gcgaggatgt tctgcccggc gatgtacccg gcgtgcgtgc tgcacaggaa
     4921 cgcgcagatc gccccgaact cgcgcgggtc gccgaagcgg ccggccggcg tcgcgttcat
     4981 cgcggcgtcg cgaacctcgt cgtacgcctt gcccgacgcc ttcgcgcgcc cttccatcgt
     5041 cgtgcgcagg cgatcggtcg cgaactgccc gggcagcagg ttgttgatcg tgacgttgtg
     5101 gcgcaccacg cgccgggcga tgccggcgac gaaaccggtg aggcccgcgc gtgcaccgtt
     5161 cgacaggccg aggatgtcga tcggcgcctt caccgcgctc gacgtgatgt tgacgatgcg
     5221 cccgaacttg cgctcgatca tcgtgtccac ggtcgccttg atgagctcga tcggggtcag
     5281 catgttcgcg tcgagcgcgc gtatccacgc cgcgcgatcc cagtcgcgaa agtcgccggg
     5341 tggcggtccg ccggcgttgt tcaccaggat gtcgggttgc ggacaggcgc ccagcacctt
     5401 ggcgcggccc tcgggcgtcg tgatgtcgca cgcgacccac gccaccttgc gcccggcgag
     5461 cgcgccgatt tcgtcggcgg ttcgcgcgac gtcggcttcg gtgcgcgcgc agatcgtgac
     5521 gtcgacgcct tctcgcgcca gcgcctcggc gcaaccgcgt cccaatccct tgctcgccgc
     5581 gcaaacgagc gcgcggcggc ctgcgatgcc aaggtccatc gaatctcctc gtcagatggt
     5641 cgcgatgagc gcatccgggc ggaacgacgc cgcggttgac gctatccaat gcgggagtgc
     5701 aaggttgcag cgagtagcat ccactatagc gcagcgcaaa cccgcgccgc gcttccgcga
     5761 cttcctcgac gagacggctc accgatgaac gacaccacgc gcaagaccga cagtgaatgg
     5821 caggcacaat tgacgcccga gcaataccgc gtcacgcgac agaaggggac cgagcggccc
     5881 ttcaccggcg aatactggga caacgaagcc gaggggacct accgctgcgt gtgctgcggc
     5941 acgccgctct tcgaatcgga tacgaaattc gacgccggct gcggttggcc gtcgttcaac
     6001 gcgccgatcg cctccgcgaa cgtgcaggag cgaagcgacg attcgtacga catgcgccgc
     6061 accgaggtcg tgtgcgcggc gtgcgacgcg catctcgggc acgtgttttc ggatggtccc
     6121 cagcccaccg ggttgcgcta ctgcatgaac tcggcgtcgc tcgagttcga gccgaagaag
     6181 taagccgccg tggacgcgcg catcgcgtag cgcgtccttg catccggcac tcgaaacgac
     6241 ttcaagatgt ttcgctaacc ggctgacgtg gaaagcttct tctatcggta aggacgcggg
     6301 caatgaacgc acccgcggcg acgatctcct cgacccccgc cgcgtggtcc atcgggtagg
     6361 cgtgccactc gagcggccat ccgccggcct gcaacacgtc gcgcgagtgt tccgcccacg
     6421 cgaacgcgat cacctggtcc tgcgtgccgt gcgccatgaa gatcggcacg tcgcgattgg
     6481 ccgcactcgc ctcggccgcg agccgctcgg gcgcgatcag atacgccgac agcgcgatca
     6541 cgccggccag ccgcgcatcg aagcgcaacc ccgcgaacag ggcgatcgcg ccgccctgcg
     6601 agaagcccgc gagcagcgtg cggctcgcca cgatgccgcg cgacgcctcg cgggcgagca
     6661 gcgcaccgac gtgcgactgc gagcgccgca cgccgtcgac gtcggcacgc tcgctgacgt
     6721 tcgcctggcg gatgtcgtac cacgcgcgca tccgcatgcc gttgttgacc gtgaccggca
     6781 tgatcggcgc gtgcgggaac aggaagcgga tcgcgagatg cttcggcaat ccgagcgacg
     6841 gaaccacctc cgaccagccg cggccatcat cgccgagacc atgcatccag atcaccgccg
     6901 catcggggtt cggcgcggtt tcgatctcga ttgcgtcgag cagttcgtcg gtcatttcgg
     6961 ttcggttgcg ggttgatcgc attctagccg acccgcccgt gatcgccggc gcggacgtgc
     7021 cgctactgca cggtgacgtg ggcgtcgcga atcacgttgc cccagcggtc gatctcgctt
     7081 tgcaacaggc gcccgaacgc ttcgggcgga tgcggcgcga ccggatcgac gcccatcgtc
     7141 cggtatcggg cgagcacctc ggggtcgcgc atcgccttca tgatctcggc gttgatccgc
     7201 tgcacgacgt ccgccggcgt cttcgcgggc acgagcaggc cgaaccacgt cgccaccgtg
     7261 agatcgggat ggccttcctc gacgatcgtc ggcacgttcg gcagttgcgg gtgacgctcg
     7321 ggcgcgagga tcgcgagcgg cttcagcttc cccgccttga tctgcggcag cgcggcgacg
     7381 acgccggtcg acatgaattg cgtgcgcccg gcgagcaggt cggacatcgc cgccgggatg
     7441 cccgcgtacg gaatcatctc catgcggatg ccgacggcgc gcgcgaacat ctcggtggaa
     7501 agatgcgtga ccgagccgac cgacgtcccc gcgtacgaca gctcgccggg gcgcgccttc
     7561 gcgtaggcga tgaactccct gagcgtggtc accggcacgc tgttcggcac gacgagcacg
     7621 aacgacgcgg tgccgaggtt cgcgaccggc aggaaatcgc gcagcggatc gtagcgaagc
     7681 ttccccggct gcagcgcggc ctgcgtggtg aacggcacgc tggtcgcaaa ccatgtgtag
     7741 ccgtcgggcg cgctgcgcgc cgcgagctcg gcgccgatgt tgccgtcggc gccggtgtgc
     7801 acctcgacga cgatcggctg gccgagtgcg ggcgagatct tctcggcgat gagccgcacg
     7861 acgacgtcga gctgaccccc ggccgggaac ggcacgatca ccttgatcgg tcgctcgggc
     7921 cacgccgcct gcgcggggtg cgccatcgat ccgagacaag ccagcatgac aaacacgaac
     7981 gtgcgcaatc gcattgcttc ctccctgatc ttcgatccct gatcccggcc gctcagtctc
     8041 gctgcgccag gcgctcgggg cggtttgcat agctgtcgtg cgcctgcgca ccgggctcga
     8101 tgcgcacgta cgacacgacg cccgtatacg cgaatgcgtc gtcgcggccg tacagcggcg
     8161 tcacatgcag tttgcggtcg cagccgacgt cgagaccctc gccgaccccg agcccgagga
     8221 tcgtcgtcgg cgacatgtcg agcgtgcccg ccgcttcgcc gttgacgaac aatgcgccct
     8281 ggccgcggcg ctcgccgcct gcacgatggt cgagcacgaa ccggttgtcg ccgcgatgca
     8341 cgcgcagccc atcgcagacg accggtctgc cttcaccgcc gtgatagacg aacgccacgc
     8401 gtccggcgcg cgcgaagagc gccatgcccg caatcgggtc accgagcgcg aagatcacgc
     8461 cttcgtcgct gccgtcgtgc gtgaacccgc attcgagccg gtagtcgcgg tcggcgacaa
     8521 gtggcgcgac ggccgcaagc gcggccgtgc cgcatccggg gtagaacgtg cgcggcgcat
     8581 tgacgctcgc ttcgagaaag ggcggcacgg tcagcgagcg gcgcacgccg cggttgtcga
     8641 gcgggtacac gtgattcgcg cgcgcatcgg cgtcgaacgc cgcgacgagg tcgcgcaatc
     8701 ggtcgggctg tccggcggcg aggtcctgcg tctcggtcgc gtcggcgcca agatcgaaca
     8761 gcatccagtt gtgaagatcg atcggcgtgc cgggcggctg cagcgacacg atcttccaat
     8821 cgtcttcgat gtaaccgcgg ttgcccgcga gctcgtaatg ctggcgcgtg cgcgctgcgg
     8881 gcgccgccgc atcggcgagc accgggcgga agctggcgcc gtcgagcgca cgcgtgcgat
     8941 agcccgcaaa gctttcggga tagccggcac cgagcacgtc gaggatcgtc ggcacgatgt
     9001 cggtcacatg cacgaattgg tgccggatcg cgccgcggtc ggcgatgccg cgcggccagt
     9061 gcgcgacgag cggcacgcga atgccgccgt tcatcgtcgt cgtcttgtag aggcgaaacg
     9121 gcgccgtcga cacatcggtc cacccgagcg aatacgccgg ccacgacgca gcgccgccga
     9181 tgccgccgct ttcgagcatc gtgcgcaccc atgccgggtc ctcgcgctgc acgaggcgct
     9241 tcgcgagatt attgaccgca ccgtcggggc cgccgatgcc gttcgcgccg ttgtccgacg
     9301 tgacgacgac cagcgtgtcg tcgagctttc cgagcgccga aagacaggcg acgacgcggc
     9361 cgatgttccg gtcgacgttg tcgacgaccg cggcatagag cgccatgaag cgtgcgtgca
     9421 gccgccgcgc ttcggcgtcg agactgtccc acgcgggcac gccgccgctt cgcgacggca
     9481 gccgccattc gcgggcgtgc aagcggagcg cgcgctgccg cgcgacgcgc gcgcttcgca
     9541 cccgatccca tccctcgtcg tacagatgcg cgtatcgcgc cagatcttcc gtcttcgcat
     9601 gcaatggcgc gtgcggcgcg ttgtagggca gatagaggaa gaacggcttg tcgggtgacg
     9661 cgctggcgtg cgccttgagc cagccgatcg ccgtgtcggt gaaatcgtcg ctgcagtaat
     9721 agccgtcgcg atagcgatcg caatcgacga acgcgttgtc gcagacgagt tgcgccggcg
     9781 cgaagtagtg cgtctcgcca ccgaggaagc cgtagaagcg gtcgaagccg cgctgcacgg
     9841 gccacgagcc gcgcgcggcc gacggcagca cgttgtaatc ggcggtgttg tgccacttgc
     9901 cggccgcata cgtcgaatac ccgtttgcac gcaacaattc ggcgagcgtc ggcgcatcgc
     9961 gcgcgatttc gccggcctgg tagccgggat agcccggcag gttgaacgtg agccagccgc
    10021 agccgacggc gtgcggattc ctgccggtca ggagtgctgc gcgggccggc gtacacatcg
    10081 gcaccgtcgt gtagttcgaa aaacgcaacc ccccggccgc gagcgcgtcg atgtgcggcg
    10141 tgcggatttc ggcgccgtag caaccgaaat cggaaaagcc gagatcgtcg agcaggatga
    10201 cgaccacgtt cggcgcacgg tccggtgcgc gctgtgcggc gggccaccaa ggcgacgatt
    10261 ccgccagcgt gcggccgatc gttccctcgt agcccgcggc cgggtcgccg cagcgcaggc
    10321 ggtcgacgct ttccctcggc ggcgctgtgt tcatcgcagt cgtcgttcgg tgttccgggc
    10381 gtcggcgcgc tttcagtcga gctcgatgcc cgcttccttc acgacctttt tccagcgcgc
    10441 aacgtcgtcc gccatgaacg ccgcgaacac cgcggccgtg ttcggcttcg ccgggtcgat
    10501 gcccatcgcc gcgagcttcg caacgacgtc gggcgccgcg agcaccttca tcacttccgc
    10561 attcacgcgc tcgacgatcg aaggcggcgt cccgtgcggc atcgcgagcc cgaaccaggt
    10621 gctcatcacg aggtcgggat ggccgagctc ggcgacgctc ggcacgtcgg gcagatgcgg
    10681 atgacgcgcc gtgtcgagca ccgcaagcgc gcgcagctcg cccgactcga ggtgcggctc
    10741 ggcgaggatc agcccgaggg tcatgaactg cacgcgcgcc gtcagcagat cgggaatggc
    10801 gctcggctgc cccttgtagc cgatcatttc catcgcgatg ccggtatcgt gctacagcac
    10861 ctcggtagcg aggtgcacga cggtgccgcg cgccgacccc gcatacgaaa gcttccccgg
    10921 ccgcgccttc gcgtacgcga cgaactcggc gagcgagtgc acgggcagcg acgcgggcac
    10981 cacgaacagg aacgggctcg tgccgacgaa cgcgacgggc gcgaaatcgc ggatcggatc
    11041 gtagccgagc gtcttcggcc gcacgctcgg ctggatcgtc gtcggcgggc tggccgcgag
    11101 ccacgtgtac ccgtcggggg ccgccttcgc gacctgctcg gtggcgatgt tgccgtcggc
    11161 gcccggcttc gcctcgacga cgatcggctg accgagcacc ggcgccaggc gatcggcgag
    11221 aagccgcgcg acgacgtcga gctggcctcc cgcgggaaac ggcacgacga agtgaatcgg
    11281 cttcgccggc cacgcgtccg cggcctgcac gaccgtcggc gcgagcgccg ccagtgcgag
    11341 cagcagggta cccatcatcg tacgtcgcat caccacgctc ctcgaatgcc ggatcatcgt
    11401 tgcaaaagtc cccagcggcc gaccgtcctg cgctcgatcg gcgtgaacac cacccggtcc
    11461 atgagcaccc aaagcaggcc gatcacgatc atgccgagga tgatcttctc ggtgcgctgc
    11521 cattgcaccg cctccatcgt catgtacccg aggccgttgc tgccggcgag catttccgac
    11581 gcgacgagcg cgcgccagcc gtaggcgacc gacgtgcgga tgccgagcag aatctgcacc
    11641 atcgcgccgg gcaggatcag gttcgcaagc agcgccagcc cgcgcccgcc atggcttcgc
    11701 accgcgcgat gcagcgcgag cggaatcgac tgcacgccga tcaccgtgtt gtagagcgcc
    11761 gagaagaaga tcgtgttcgc gatcacgaaa atcaccgagc cgttgcccat gccgaaccag
    11821 atgatcgtca acggcagcca cgcgatgccg gcgat
//
LOCUS       DASSVX010000214         3162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_858946, whole genome shotgun sequence.
ACCESSION   DASSVX010000214 DASSVX010000000
VERSION     DASSVX010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858946"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1711
                     /locus_tag="VFO53_09245"
     CDS             <1..1711
                     /locus_tag="VFO53_09245"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET8876499.1"
                     /translation="IAALPSNDAQLAAALRSAPTVLAMVGALAPTAQALRAVPILAGG
                     AQDDADVRARATARLTSYPAALSSVALLTNAAHGWGLISAEDTRGVVRQIALVANVNG
                     TLVPGLAVEMWRVALGARALRLSFGQGVVRDVQIGRDSFRTEADGSARPWFSTPRPGR
                     FVSALDVLNGTADGDLLRRSFVLIGVTGLALGDYVWTPVAKMPGVEVHAQVLENMNDR
                     TFLVRPRVAPLVEAAALLVAGGLLIAWMPGWPVRYGTLAAVACVLAFGALSYAAFRQQ
                     RLLFDAATPALAVALLFATLLALQLADSTRHRKALQRVLQREREAAARVAGELQAARR
                     VQLDTLPRAEALRDARVELAATMEPALEVGGDLYDFYLLDDDRLFFMLGDVSGKGLAA
                     SIFMAVSKALCKSTMLRAKDADLGLLLARANAEVGRDNPAAFFVTAFAGVLELRTGML
                     DYCNAGQENPWRREASGGTVTRLAAGGGPPLCVVDDFPYRSMRLALQPGDLVCVVSDG
                     VTEASDGGGAMYGASRVATVLAPVTTAAQAIDAIRDDVARFAAGAAPSDDRTVLALQW
                     RGP"
     gene            1810..2241
                     /locus_tag="VFO53_09250"
     CDS             1810..2241
                     /locus_tag="VFO53_09250"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clan AA aspartic protease"
                     /protein_id="HET8876500.1"
                     /translation="MSIVYAEVRLANAARPDLEEITVTALVDSGAIDLIVPEHIAVQL
                     QLTDLRPREVTIADGSRRQVRYVGPVRIDAQGRDCMTAACVMGDQVLLGAIPLEAMDM
                     IIHPRLQRLIPNPENPNLPGFIVKSAPNRPRSANRLVRGGP"
     gene            complement(2260..2859)
                     /locus_tag="VFO53_09255"
     CDS             complement(2260..2859)
                     /locus_tag="VFO53_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET8876501.1"
                     /translation="MGASRLALRCLLFALAALCCGCATHERIILLPESGGGPTSLAVR
                     QGGREVLLDRPYAAAELTIADPFRYDASAAEVQATFGEALSAQPARAVHFTLNFIEGS
                     DELTEESKETFERMFADLAQRRVRDIVVVGHTDAVGSDQYNDALAKKRADAVSALLVA
                     RGVAASDIVAIGRGKRDLLIPTPSGIAEPRNRRVEVVVR"
     gene            complement(2849..>3162)
                     /locus_tag="VFO53_09260"
     CDS             complement(2849..>3162)
                     /locus_tag="VFO53_09260"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET8876502.1"
                     /translation="AGVGTRLQTSDVVTTGSDGSVGITMTDDSLLSAGPDSVLALDRY
                     DFDDVTSAGRFDTSLRKGTLAVVSGRLARQSRDAMTVRTPSAVLGVRGTEFVVSVGDG
                     R"
BASE COUNT          462 a         1061 c         1119 g          520 t
ORIGIN      
        1 catcgcagcg cttccgtcca acgacgcgca actcgctgct gcattgcgtt cggcacccac
       61 ggtgctggcc atggtcggcg cgctcgcgcc gacagcgcag gcgcttcgcg ccgtgccgat
      121 cctggcggga ggtgcgcagg acgacgccga cgtcagggcg cgggcaaccg cacgactcac
      181 gagctatccg gcggcgttgt cgagcgttgc cctgctgacg aacgccgcgc acggctgggg
      241 gctgatttcc gcggaagaca cgcggggcgt ggtgcgccag atcgcgctcg tcgcgaacgt
      301 gaacggcacg ctggtgcccg ggctcgccgt cgaaatgtgg cgcgtggcgc tcggcgcgcg
      361 ggcattgcgg ctttcgttcg ggcagggcgt ggtgcgcgac gtgcaaatcg gccgcgacag
      421 ctttcgcacc gaggccgacg gcagcgcgcg accctggttc tcgacgcctc gccccgggcg
      481 gttcgtctcg gcgctcgatg tgctgaacgg caccgcggat ggcgatcttc tgcgccgcag
      541 cttcgtgctg atcggcgtga cgggtctcgc gctcggcgat tacgtatgga cgccggtggc
      601 gaagatgccg ggcgtcgaag tgcacgcgca ggtgctggag aacatgaacg atcgcacgtt
      661 cctcgtgcgc cctcgggtcg cgccattggt cgaggcggcg gcgctgctcg tcgcgggtgg
      721 tctgctcatc gcctggatgc ccggctggcc ggtgcgctac ggcacgttgg cggccgtcgc
      781 gtgcgtgctt gcgttcggcg cgctgtcgta cgcggcgttt cgccagcagc ggttgctgtt
      841 cgatgccgca acaccggcgc tcgccgttgc gctgctcttt gcgacgctcc tcgcattgca
      901 gctcgccgat tcgacgcgtc atcgcaaggc actgcagcgc gtgctgcagc gggagcgcga
      961 ggccgcggca cgcgttgccg gcgaattgca ggcggcgcgg cgcgtgcagc tcgatacgct
     1021 gccgcgtgcg gaggcgttac gcgatgcgcg cgtcgagctc gccgcaacca tggagcccgc
     1081 gctggaagtg ggcggcgacc tgtacgactt ttacctgctc gacgacgacc ggctgttctt
     1141 catgctcgga gacgtgtcgg ggaagggcct cgccgcgagc atcttcatgg ccgtgagcaa
     1201 ggcgttgtgc aagagcacga tgctgcgcgc caaggacgcc gacctcgggc tgctgctggc
     1261 gcgcgcgaac gccgaggtcg gccgcgacaa tccggcggcg ttcttcgtca ccgcgtttgc
     1321 gggtgtcctc gagctgcgaa cggggatgct cgattactgc aacgccggcc aggaaaatcc
     1381 gtggcggcgc gaggcgtcgg gcggcacggt gacccggctc gccgctggcg gtggaccgcc
     1441 gctgtgcgtc gtcgacgatt tcccctatcg ctcgatgcgc ctcgcgttgc agccgggcga
     1501 cctcgtgtgc gtggtgagcg acggcgtcac cgaggcgagc gacggcggcg gtgcgatgta
     1561 cggcgcgtcg cgcgtggcaa cggtgcttgc gccggtgacg accgccgcgc aggcgatcga
     1621 tgcgattcgc gacgacgtgg cccggttcgc cgcgggtgcc gcgccctcgg acgacaggac
     1681 ggtgctcgcg ctgcaatggc gcggcccgtg acatgacgat cggtgaaatc cgctaccccg
     1741 ttcgaccgat ggcgcgtgtc gcggaccgat gcttcaatag cgccacatgc caccggccga
     1801 ggagttgcga tgtcgatcgt ctatgccgaa gttcgcctgg caaatgccgc gaggcccgat
     1861 ctcgaggaga tcacggtcac cgcattggtc gatagcggcg ccattgatct catcgttccc
     1921 gagcatatcg cggtgcaatt gcagctcacc gatctccggc cgcgcgaggt cacgattgcc
     1981 gacggcagtc gcaggcaggt tcgctacgtc ggcccggtca ggatcgacgc gcaggggcgc
     2041 gattgcatga ccgcggcgtg cgtgatgggc gatcaggtgc tgctcggtgc gattccgctc
     2101 gaagccatgg acatgatcat ccatcccagg ttgcagcgcc tgattcccaa tcccgaaaat
     2161 ccgaacctgc ccggattcat cgtgaaatcg gcgccgaacc ggccgcgcag cgccaatagg
     2221 ctggtgcgcg gcggcccttg attgcgtgcg agtcgggcgc tagcgcacca cgacctcgac
     2281 gcgccgattg cgcggctcgg cgatgccaga aggcgtcggg atcagcaggt cgcgcttgcc
     2341 gcggccgatc gccacgatat cgctcgcagc cacgccgcgc gcgacgagca acgcgcttac
     2401 tgcatcggcc cgctttttcg cgagcgcgtc gttgtactgg tcgctgccca ccgcgtcggt
     2461 atggccgacg acgacgatgt cgcgaacgcg gcgctgcgcg agatccgcga acatgcgctc
     2521 gaacgtttcc ttcgattcct cggtgagctc gtcgctgcct tcgatgaagt tcaacgtgaa
     2581 atgcacggca cgcgcgggct gcgccgaaag tgcctcgccg aacgttgcct gcacttcggc
     2641 tgcgctcgcg tcataccgga acggatcggc gatcgtgagc tcggccgcgg catagggccg
     2701 gtcgagcagc acctcgcgcc cgccctgccg cacggcgagc gacgtcggcc cgcctcccga
     2761 ttcgggcagc aggatgatcc gctcgtgcgt cgcgcaaccg cagcacaagg cggcgagcgc
     2821 gaacagcagg caacgaagcg cgaggcggct agcgcccatc gcccacgctg acgacgaact
     2881 cggtgccgcg aacgccgagc accgccgacg gcgtgcgcac ggtcatcgca tcgcgcgatt
     2941 gcctggcaag ccgccccgag acgacggcaa gcgtgccctt gcgcagcgac gtgtcgaagc
     3001 ggccggcgct cgtcacgtcg tcgaagtcgt agcgatcgag tgcaagcacg ctgtccggtc
     3061 cggcggacag cagggaatca tcggtcatcg tgattccgac cgagccgtcg ctgcccgtcg
     3121 ttaccacgtc ggacgtttgc agccgcgtac cgacgccggc cg
//
LOCUS       DASSVX010000215         3481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_442321, whole genome shotgun sequence.
ACCESSION   DASSVX010000215 DASSVX010000000
VERSION     DASSVX010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442321"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..1361)
                     /locus_tag="VFO53_09265"
     CDS             complement(285..1361)
                     /locus_tag="VFO53_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HET8876503.1"
                     /translation="MINPGIRKLVAAVAAVALLGHTVFAEAAAAFKIVTASERGTYIK
                     IGQDLAQYIAPSADIQLEALPSAGSAENVRRLRYEPGVKLALVQSDVYQAFLDQAAAG
                     NAEARELIMPLRVVMPLYNEEIYFVVRADSPLNSVHDIRGMRINAGPVGSGTALTTTT
                     LYKLMFNEPLADANASFLSNEDALAKLVTDRSVDVVAIIAGQPAKVLTDMTAESRKYV
                     KLLRFDPNDPASQAALQTYFPATVRATSYPNLLTEDLPALAVKAYLVTYDFNLGSTRD
                     YLRRLARSLCRNFFVLQSNGHPKWREVNLALPDLGKGWGYYAPTTRELQACIASRPRT
                     AAGGAAPPTSASCSQQERVLGLCK"
     gene            1534..2052
                     /locus_tag="VFO53_09270"
     CDS             1534..2052
                     /locus_tag="VFO53_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876504.1"
                     /translation="MLDRVRAGACRAETAGDEAASAPLPRLTCGWASLAFGPTAFVDV
                     SVVIAVFCAVETVDAPVRAAVAVLSSRGLAAGSLMAASRGTDGCGAAIVASLTPPGEG
                     AAASPETAGDVAVNALAAGGCDVACTAFDAASVDGMMTGEAVVGAAITAVVAIGGALA
                     SGDAAERIDVRS"
     gene            complement(2737..>3481)
                     /locus_tag="VFO53_09275"
     CDS             complement(2737..>3481)
                     /locus_tag="VFO53_09275"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET8876505.1"
                     /translation="IIHDYTREAGVRNLEREIGNVLRNVAVRIAEGTAQKTTIDATDL
                     QPILGARKFDAEVAMRSSVPGVATGLAWTPVGGDILFVEAARMPGSGKLILTGQLGDV
                     MKESAQAALSLVKARAEQLGVAQALEKSDIHIHVPAGATPKDGPSAGVAIFVALTSLL
                     TGRPVRSDVAMTGEISLRGLVLPIGGVKEKVLAALRAGIKTVMLPVRNQRDLDDIPTD
                     ARAQLEFVWMESVDDAIATALPAVAAAIA"
BASE COUNT          571 a         1148 c         1167 g          595 t
ORIGIN      
        1 cggccgtttt cgtcatggca cgagcttcct gcggtggcgg ccggacaagg cgccgcgaca
       61 gtgcacgttc gatcaggtac agcacgaatc cgccggcacg ctgctcgcgt cgctcgccga
      121 cggtccgaca tgaacgcgga acaggctgtc gaaaagcgtc acgaggaggt cgagatgcga
      181 ggcgcgagga cgcgagcaac cggagcgtac tcaaacgtac gtgaggattg cgagcgcccg
      241 agcaacaaag cagatcggcc ccgcagtagc tttgcgacag cctgttactt gcagagaccc
      301 aggacgcgct cctgctgcga gcaggacgcc gatgttggtg gcgccgcgcc gccggccgca
      361 gtgcgcggcc gcgacgcgat gcacgcctgc aactcgcgcg tcgtcggcgc gtagtaaccc
      421 caccctttgc cgagatcggg cagcgcgaga ttcacctcac gccatttcgg atgaccgttc
      481 gactgcagca cgaagaaatt gcggcagagc gaacgcgcga gtcgccgcag gtaatcacgc
      541 gtgctgccga gattgaaatc gtacgtgacg agatacgcct tcacggccag cgccggcaag
      601 tcttcggtca gcagattggg atagctcgtc gcacgaaccg tggccggaaa atacgtttgc
      661 agcgccgcct ggctcgcagg atcgttcgga tcgaagcgca gcagcttcac gtatttgcgt
      721 gactccgccg tcatgtccgt caataccttc gctggttgcc cggcgatgat cgcgacgacg
      781 tcgaccgagc gatcggtgac gagcttcgcg agtgcgtcct cattcgacag gaacgacgcg
      841 ttcgcatcgg cgagcggctc gttgaacatc agcttgtaga gcgttgtcgt cgtcagcgcc
      901 gtgccggaac ccacgggccc ggcgttgatg cgcatgccgc gaatgtcgtg gacgctgttg
      961 agcggcgaat cggctcgaac gacgaaatag atttcctcgt tgtagagcgg catcacgacg
     1021 cgcagcggca tgatgagctc gcgcgcttcc gcgtttcccg cagccgcctg gtcgagaaat
     1081 gcctggtaga cgtcggactg cacgagcgcc agcttcacgc ccggctcgta acgtagccgg
     1141 cgaacgttct ccgcggagcc agctgaaggc agtgcttcga gctggatgtc ggccgacggc
     1201 gcgatgtatt gcgcgaggtc ctgaccgatc ttgatatacg taccgcgctc cgacgccgtc
     1261 acgatcttga aagcggcggc ggcttcggcg aacacggtgt ggccgagcaa tgcaaccgcg
     1321 gccactgcgg cgactagttt gcgaatgccc gggttgatca tccgcacgtc ctagcggctg
     1381 cagaggccga gtgcggcggt actgtccgtg cacgacgcgg caggcatcgg tggaaccggc
     1441 gtgcgcgctg ccggcggcgg tgccgcgacc acgggcgctg cgggctgcca ttgccggggg
     1501 gccgcgtagc gcggagtcgt cgttgcgcgc gcagtgctcg atcgagtccg tgccggcgca
     1561 tgccgcgccg aaacagcggg cgatgaggct gcatcggcgc cactgccgcg attgacctgc
     1621 ggctgggcgt cgctggcgtt cggtcccacc gcgttcgtcg atgtatccgt cgtgattgcc
     1681 gtattttgcg ctgtggaaac cgttgacgcg cccgtccgcg ccgccgttgc ggtactttca
     1741 tcgcgggggc tcgccgcagg ttcgttgatg gcagcatcgc gcggaaccga cggttgcggt
     1801 gcggctatcg ttgcgtcctt gacgccgccc ggcgaaggcg cggcagcgtc accggagaca
     1861 gctggcgacg ttgccgtcaa cgcattggcc gcaggcggct gcgatgtcgc ttgcacagca
     1921 ttcgacgcgg cctcggtcga cggcatgatg acgggtgagg ccgtcgtcgg cgccgcgatc
     1981 actgcagttg tcgctatcgg cggcgcgttg gcctcgggtg acgccgcgga gcggatcgac
     2041 gtccgctcat agccgtagta aacgacgccc gctgcaacga ccagaagtgc ggcagcgatg
     2101 gcaattcgcg gcccgcggcg cagtgccacg cccggatcgg gcccggcgtc gatgacgggc
     2161 gtgtcatcca tcacgcggag cggcagcatg tcgaccgcgc gcggcggcgg cggtgccgcg
     2221 ggcgtcacat cgcccttcat cgcgcgagcg acttcgaccg cctgcatcga atcgcgccag
     2281 aaggcgtcga ggcgctcggc cgccgatgcg agctctcgcg gagatgcgaa cgctgtcttg
     2341 ccggcgccca cctccggcag cgatgcggcg gagtcggcgg cgcttgcggc accgggctgc
     2401 gaacgcatca ctggggatga cggggcctcg tgcgtgcctt cggctttcgc agtgctcacg
     2461 accgcggctg cagcggcacc gagcagcgtg ccgcactggt gacagaacgt cgctgcgacg
     2521 tcgttgatag cctcgcactg cgaacacggt cgcaagccga gaccggcgcc gcattcgttg
     2581 cagaatttgg cgtcggccgg attggcgtga tcgcagaacg aacagttgac ggaaggcatg
     2641 gggtgacccg actgcgacct tgcatcatgc atcggattcc cgcccgcgtg ttggcggccc
     2701 gttcaacggg ccaccaccgt tgagacgaac taccggctac gcgatcgccg ccgccaccgc
     2761 ggggagcgcc gttgcaatcg catcgtcgac gctttccatc cacacgaact cgagttgcgc
     2821 gcgtgcgtcg gtcggaatgt cgtcgagatc gcgctgattg cgtaccggca gcatcaccgt
     2881 cttgatccct gcacgcagcg ccgccagcac cttttccttg acgccgccaa tcggcagcac
     2941 gagaccgcgc aagctgatct caccggtcat cgccacatca ctgcgcaccg gccgtccggt
     3001 cagaagcgac gtcaatgcaa cgaagatcgc cacgcccgca ctcggaccgt ctttcggcgt
     3061 cgcacccgcg ggcacatgaa tgtggatgtc cgatttctcg agcgcttgcg cgacaccgag
     3121 ctgctcggcg cgtgccttga cgagcgacag cgcggcctgc gcgctctctt tcatcacgtc
     3181 gccgagctgc ccggtcagga tcagtttgcc cgaccccggc atccgcgccg cctcgacaaa
     3241 cagtatgtca ccgccgaccg gcgtccacgc aagccccgtt gcaacgccgg gcacgctgct
     3301 gcgcatcgcg acttcggcgt cgaacttgcg cgcgccaagg atcggctgca gatcggtcgc
     3361 gtcgatcgtc gtcttctgtg ccgtgccctc ggcgattctc acggcgacgt tgcgcagcac
     3421 gttgccgatc tcgcgctcga gattgcgcac gcccgcttcg cgcgtgtagt cgtgaatgat
     3481 c
//
LOCUS       DASSVX010000216         3495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_442372, whole genome shotgun sequence.
ACCESSION   DASSVX010000216 DASSVX010000000
VERSION     DASSVX010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442372"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1536
                     /locus_tag="VFO53_09280"
     CDS             64..1536
                     /locus_tag="VFO53_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HET8876506.1"
                     /translation="MSTHDLPKPSLLAAIAHKVMLQRGLEPDFSEAVQSELAAIDGPA
                     TKADADIRDLRGLPWCSIDNDDSRDLDQLSVFGANGAEANILVAIADVDALVASGSAI
                     DAHAAINTTSVYTAAGIFPMLPEKLSTDLTSLGEDDERLAIVVDMNVNDAGEVTASTV
                     YRAVVRNHAKLAYNAVAAWLDGAGPAPQRLASVAGLNAQLRAQDAVAQCLKRSRSEKG
                     ALALETLEVRPVFEAGTLADLRPDAKNRAKELIENFMIAANVATARFLGSHGFASLRR
                     VLRSPERWQRIVELAAGLGRALPAAPDAAALDAFLLERRTSDPAGFADLSLAVVKLLG
                     SGEYDVELPGGGGEGHFGLAVKDYSHSTAPNRRFPDLIAQRLLKAALAGRAPPYSNDA
                     LRVLAAHCTQQEDNAAKVERQVQKSAAALLLQRRIGAKFDAIVTGASSKGTWVRISHP
                     TVEGRVVRGFDGLDVGDAVRVELVHTDASRGFIDFARAGR"
     gene            1538..2320
                     /locus_tag="VFO53_09285"
     CDS             1538..2320
                     /locus_tag="VFO53_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET8876507.1"
                     /translation="MTQKPRTRKKVAYKDDAFLDSDDARPLRILAEYLQPMQAFRREN
                     VADTVVFFGSARLAQDGPLGRYYDDARELARLLTQWALSLPANCARYVVCSGGGGGIM
                     EAANRGAADAGGRSVGLNIGLPHEQRPNPYITPELTFEFHYFFMRKLWFAHLARALVV
                     FPGGFGTLDEMTEILELAQTGKLARRIPVVLYGRSYWNEIIDFDALVRHGMIAAEDLT
                     LFSYADDPQSALKLLQRQMSPTAEPEKKPDFAPSRMPPGAAG"
     gene            complement(2396..3067)
                     /locus_tag="VFO53_09290"
     CDS             complement(2396..3067)
                     /locus_tag="VFO53_09290"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET8876508.1"
                     /translation="MATSTMHAEPYEFEFDPRTTALIVIDMQRDFVEPGGFGEALGND
                     VTPLQAVIGPCRRMLDAARERGMMIIHTREGHSADLADCPPSKIVRGRGEKRIGDPGP
                     MGRILVRGEHGHDIVPELYPVAGEVVVDKPGKGAFCATDLDLILHNRGIRSLIVCGVT
                     TEVCVNTTVREANDRGYECVVLSDCVGSYFPEFQRVALEMIKAQGGIFGWVSDSTRAL
                     VALDA"
     gene            complement(3120..>3495)
                     /locus_tag="VFO53_09295"
     CDS             complement(3120..>3495)
                     /locus_tag="VFO53_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HET8876509.1"
                     /translation="IPNIAEWAKTQIDGALAAAGTSAAQLGPAKLEATGVLYRGLESL
                     GGGSVLAGMVLGAIAVFIIDGKLRTAAVWSFAGAILAYFGLIHGAQLGWAVSPLVALG
                     YALFGATCLALGAREPNALANA"
BASE COUNT          577 a         1188 c         1171 g          559 t
ORIGIN      
        1 tcgctgtatg caaccttgcc tgcaccgcca ccgccgcata cgaaaaagcg ggtcatgcga
       61 ccgatgagca cacacgacct gcccaaaccc tcgctcctcg ccgccatcgc ccacaaggtg
      121 atgctgcaac gtggactgga acccgacttt tccgaagcgg tgcagtccga gctggcggcc
      181 atcgacggcc cggcgacgaa agcggacgcc gatattcgcg atctgcgcgg gcttccgtgg
      241 tgctcgatcg acaacgacga ttcgcgcgat ctcgaccagc tgagcgtttt cggtgccaac
      301 ggcgccgagg cgaacattct cgttgccatt gcggacgtgg atgcgctggt cgcgagcggc
      361 tcggcgatcg acgcacacgc cgcgatcaac accacctcgg tctacaccgc cgccggcatc
      421 ttcccgatgc tgccggagaa gctgtccacc gacctcacct cgctgggcga ggacgacgag
      481 cgactcgcga tcgtcgtcga catgaacgtg aacgatgcag gcgaggtgac cgcgagcacc
      541 gtgtaccgcg ccgtggtgcg caaccacgcc aagctcgcct ataacgccgt cgcggcatgg
      601 ctcgatggcg cgggacctgc accgcaacgt ctggcgagcg tcgccggtct caacgcgcag
      661 ctgcgcgcgc aggatgccgt cgcgcagtgt ctcaagcgat cccgctccga gaagggcgcg
      721 ctcgcgctcg aaacgctcga ggtgcggccg gtgttcgagg cgggcacgct tgccgacctg
      781 cgtcccgacg cgaagaaccg cgcgaaggaa ctgatcgaga atttcatgat tgcggccaac
      841 gtcgcaactg cgaggttcct cggcagccac gggtttgcgt cgctgcgcag ggtgcttcga
      901 tcgccggaac gatggcagcg gatcgtcgaa ctcgccgcgg ggctcggtcg cgcgttgccc
      961 gcggcaccgg acgccgctgc gctcgacgcg ttcctcctcg agcggcggac gagcgatcct
     1021 gccggcttcg ccgacctctc gctcgccgtc gtcaagttgc tgggctcggg cgaatacgac
     1081 gtcgagctcc cgggcggcgg cggggaaggg cacttcggcc tcgcggtgaa ggattactcg
     1141 cattcgacgg cgccgaaccg gcgcttcccg gatctcatcg cgcagcggct gctcaaggcc
     1201 gcgcttgccg gccgtgcgcc gccgtattcg aacgacgcgt tgcgcgtgct cgcggcgcac
     1261 tgcacgcaac aggaagacaa cgcggcgaag gtcgagcgcc aggttcagaa gtcggctgcg
     1321 gcattgctgc tgcagcggcg catcggcgcg aagttcgacg ccatcgtgac gggcgcgtcg
     1381 tcgaaaggca cgtgggtgcg catctcgcat cccaccgtcg aagggcgcgt cgtccgcggg
     1441 ttcgatggcc tcgacgtcgg cgatgcggtg cgggtcgagc tcgtgcacac cgacgcatcg
     1501 cgcggcttca tcgactttgc acgcgcggga cgatgagatg acgcagaaac cgcgaacacg
     1561 caagaaggtt gcatacaagg acgatgcgtt cctcgacagc gacgacgcga ggccgctgcg
     1621 catccttgcc gagtacctgc agccgatgca ggcgtttcgc cgcgagaacg tggcggacac
     1681 cgtcgtcttc ttcggctcgg cgcggcttgc gcaggacggt ccgctgggtc gctattacga
     1741 cgatgcgcgc gagctggcgc gcctgctgac gcagtgggcg ctgagcctgc ccgcgaactg
     1801 tgcgcgctat gtcgtttgtt ccggtggtgg tggcgggatc atggaagcgg cgaatcgcgg
     1861 cgcggccgat gcaggcggcc gcagcgtcgg tctcaacatc ggcctgccgc acgagcagcg
     1921 gcccaacccg tacatcacgc cggaactgac attcgagttt cactacttct tcatgcgcaa
     1981 gctgtggttc gcgcatctcg ctcgcgcact ggtcgtattc ccgggcgggt tcgggacgct
     2041 cgacgaaatg accgagatcc tggaactggc gcagacgggg aagctcgcgc gccgcattcc
     2101 ggtcgtgttg tacggacgta gctactggaa cgagatcatc gacttcgacg cgctcgtccg
     2161 tcacggaatg atcgcggccg aggacttgac gctgttcagc tacgccgacg atccgcagag
     2221 cgcgttgaag cttcttcagc gccagatgtc gccgacagcc gaacccgaga agaagcccga
     2281 tttcgcgcct tctcggatgc cgccgggcgc cgccggttga gcgttgacgg cgctcgtcag
     2341 cacggcgcgt tccgcgctgg caccgcgtcg tggttgacgc acaaggctcg tggtctcacg
     2401 cgtcgagcgc gaccagtgcg cgcgtcgaat cggacaccca gccgaagatg ccgccctgcg
     2461 ccttgatcat ctcgagcgcg acgcgctgga actccggaaa gtacgagccc acgcagtcgg
     2521 acaggacgac gcattcgtag ccgcggtcgt tcgcctcgcg caccgtcgtg ttcacgcaca
     2581 cctcggtcgt gacgccgcag acaatcagcg accggatccc gcggttgtgc aggatcaggt
     2641 cgagatcggt tgcgcagaac gcgcccttgc ccggcttgtc gacgacgacc tcgccagcga
     2701 cggggtagag ctcgggcacg atgtcgtggc catgctcgcc gcgcacgagg atgcgcccca
     2761 tcggaccggg atcgccgatg cgcttctcgc cgcggccgcg cacgatcttc gacggcgggc
     2821 aatcggcgag atcggcgctg tgtccctcgc gggtatggat gatcatcatc ccgcgctcgc
     2881 gggccgcgtc gagcatccgc cggcacggcc cgatcaccgc ctgcagcggc gtcacgtcgt
     2941 tgccgagcgc ttcgccgaaa ccgcccggct cgacgaaatc gcgttgcatg tcgatgacga
     3001 tcagcgcggt ggtgcgcggg tcgaactcga actcgtacgg ctcggcgtgc atcgtcgacg
     3061 tggccatcgc tcgactcctc gtaaatgaat caccggcgtc tgccgcgccg cttgcgggtt
     3121 cacgcattcg ccagcgcgtt cggctcgcgc gcgccgagcg ccaggcacgt cgcgccgaac
     3181 agtgcgtagc ccagcgcgac gagcggcgac accgcccagc cgagttgcgc gccgtgaatc
     3241 agcccgaagt acgcgaggat cgcgccggcg aacgaccaca ccgcggcggt gcgcagcttg
     3301 ccatcgatga tgaagacggc gatcgcgccg aggaccatgc cggccagtac cgagccgccg
     3361 ccgagcgatt cgagtccccg atagagcacg ccggtcgcct cgagcttggc gggaccgagc
     3421 tgcgcggcgg acgtacccgc cgccgcgagc gcgccatcga tctgcgtctt cgcccattcg
     3481 gcgatgttgg gaatc
//
LOCUS       DASSVX010000217        14182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1358974, whole genome shotgun sequence.
ACCESSION   DASSVX010000217 DASSVX010000000
VERSION     DASSVX010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 14182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14182
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358974"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1849)
                     /locus_tag="VFO53_09300"
     CDS             complement(17..1849)
                     /locus_tag="VFO53_09300"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HET8876510.1"
                     /translation="MNSESLSALAASVHAPSGVRHRRLVEWVAQIASLTAPETVVWCD
                     GSEEEYVSSCERMVAAGTLHRLDPARRPNGFLARSDPSDVARVEDRTFICSEREEDAG
                     PTNNWVEPAKMRRTLRGLFEGCMRGRTMYVVPFSMGPLGSPIAQIGVEITDSPYVVAN
                     MRMMTRMGARVHDVLGEDGSFIPCVHSVGVPLAPGAQDVAWPCNPANKYIVHFPEVRE
                     IWSFGSGYGGNALLGKKCLALRIASVMGRDEGWLAEHMLILGVTSPAGVKRYVAAAFP
                     SSCGKTNFAMLIPPAGLEGWRVTTIGDDIAWIKPAADGTFHAINPEAGYFGVAPGTSY
                     ASNPNAMETLRANVIFTNVALTDDNDVWWEGMTKTPPAHLVDWRGNAWEPGCGRPAAH
                     PNARFTVSATQCPSLDPEWNNPAGVPLAALVFGARRSDTVPLVTEARTWDEGVYKAAT
                     MGSETTAAATGKVGEVRRDPFAMLPFCGYHMGDYFRHWLEMGRAVSHPPRMFSVNWFR
                     KDGEGRFVWPGFGQNMRVLKWIFERCAGEAGATESSLGLVPEYDHLDWSGLDFGASRF
                     SAIMGVDPTQWQREIDSHNALFDRLGEKRPDRLMRERDRLARKL"
     gene            complement(1975..2871)
                     /locus_tag="VFO53_09305"
     CDS             complement(1975..2871)
                     /locus_tag="VFO53_09305"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET8876511.1"
                     /translation="MAAERLRLLIGGALPALVATDCMAHVAGAVTHLHVHIDAVLASA
                     LLASAVLYARGVTRLWRKAGVGRGIRAVDVARFGLGIATLAAALLPPIDALAGRSFAT
                     HMIEHEMLMVIAAPLFVLARPLEASAWALPGGTRRAMVAFTRSTLVRRIVRSTGAPVF
                     ATCVHALALWIWHLPPLFLAALASTPLHVFQHVCFFASALAFWWAMFGGAMRTPGAVS
                     VACLFATMLHTSALGALLAFAPSPWYVHDAGPRAFGLTPLEDQQLGGLIMWVPCGLAY
                     VVVALSIVGTWLSPSAVRRRPG"
     gene            complement(2844..3170)
                     /locus_tag="VFO53_09310"
     CDS             complement(2844..3170)
                     /locus_tag="VFO53_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876512.1"
                     /translation="MRVWFALLAVPLLALADQSASYVLSVWSCDHQNTVAMHAVHVPF
                     LLVAATGTIAAWQSWRRSSAMKADNETLARRHFLAGLATGAAALSVLVILAMWAVSWL
                     LSACVY"
     gene            complement(3173..3871)
                     /locus_tag="VFO53_09315"
     CDS             complement(3173..3871)
                     /locus_tag="VFO53_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit III"
                     /protein_id="HET8876513.1"
                     /translation="MARSASIDDRVDPAREDKAIRRRRQSTPASIPAVRRGDALDVSG
                     LPSYGFGTRSLMWWGTAGMSAIEGMGFAMMIVVYFYLKSRSPLWPNEGGPPGLLWGTV
                     NLGLACLSIVPNLMANKAADDHDLARVRLWLIVFVIYGLALLGVRWLEFDALNVRWDA
                     SAYGSAVWVLLGLHTFNLVTNYADSLVVAAVMFKRPVDGKRFVDVAENAGYWWFIVLT
                     WIPIYAVVYWGPRF"
     gene            complement(3874..5883)
                     /gene="ctaD"
                     /locus_tag="VFO53_09320"
     CDS             complement(3874..5883)
                     /gene="ctaD"
                     /locus_tag="VFO53_09320"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET8876514.1"
                     /translation="MTADAGSLSAPGATRSQHALREQADAAVGRGETLLDGAGFGSKA
                     ALALEHAWRDEPGFIGWLKTNDHKRIGVRFVVTAFVFFCAAGLLAAVMRTQLAFPDNH
                     VVGPDLYDQLFTMHGTTMMFIFAVPIMQGMGVYLVPLMVGTRNTAFPRMTACAYWLYL
                     LGALSLWTGFAINTGADAGWFSYVPLAGPQYGIGKRADVWNNLINLTEAMGLMVAVDL
                     ATVILKMRAPGMSLRRMPVFVWSSLVASMMIIFAMPSVMLAANLAQFDRTFGTHFFNP
                     AEGGDVLIWQHLFWFFGHPEVYFIFIPALGFVSMIIPTFARRPLFGHAAVVLSLIATG
                     FLAFGLWVHHMFATNLPELGKSFFTAMSFMIAIPTAAQIFCWIATLWTGRLNVRVPLL
                     FVLGFFAILVLGGLTGIMVGAVPFDLQVHDSYFVVAHLHYVLLGGAVFPLFGAFFYWF
                     PKFTGRMLDERLGRWQFWLFFVGFNVAFFPMHILGLEGMPRRVYTYGAETGWGTLNLI
                     ATIGATIIAVSVLLFVVNFARSVAKGRLAGDNPWAASTLEWATHSPPPAFNFVALPVV
                     ASREPLWAREADEPTHVSGLSAQTREGLVTTVLDALPDVRYSYPSPTVWPFIAAVTVD
                     IWLFWSNVTPSGLYYGLIPPAIAFIAWYWPKRKETAEELLLQRRP"
     gene            complement(5880..6848)
                     /gene="coxB"
                     /locus_tag="VFO53_09325"
     CDS             complement(5880..6848)
                     /gene="coxB"
                     /locus_tag="VFO53_09325"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET8876515.1"
                     /translation="MIGGLHDVQLPAGAQARAIVDLWWWLLPICTLVFVAVIMALALA
                     LRRAPRVAQDAAPDVSSLWQREPRVVRTVWVAIAVSIVLLLVLLAASVLTDRAIARLP
                     LADALHIELTGYQWWWAARYDDADASRIFTTANELHVPVGRPVIVSLAGADVIHSFWV
                     PSLHGKKDLIPGRDATVSFRADRPGIYRGQCAEYCGYQHAHMVLYVVADAPDVYAQWA
                     ARQREPAAAPVTASQVRGRDLFVSRSCAMCHAIGGTTAQARNAPDLTHVASRMTIGAG
                     TLDNTPSMRAAWILDPQQFKPGANMPAQSFAPEELAALNAYLGSLK"
     gene            complement(6845..7402)
                     /locus_tag="VFO53_09330"
     CDS             complement(6845..7402)
                     /locus_tag="VFO53_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET8876516.1"
                     /translation="MKRDRAHAVAITALAALALAGCQRESRDFEPPTKAVSPPPIRMS
                     AIQPGAIATNTSVERKYEDNAYALSEGKRLFGWYNCTGCHANGGGDKGPALMDNVWVY
                     GSDPANIYATIVEGRPNGMPSFGGHVPDNEVWELVAYVRSLSGLASSSAAPNRADAMQ
                     VRKPESQTEQQTPVVAAPSGASERP"
     gene            complement(7399..8211)
                     /locus_tag="VFO53_09335"
     CDS             complement(7399..8211)
                     /locus_tag="VFO53_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017263758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HET8876517.1"
                     /translation="MSSRCLEIRALALCALALLVSAPTAARELRVCADPGNLPYSDRD
                     ERGFENRIASLVAHDLRADLRYYWLPQWRGYARKTLQQHRCDVIPGIAQADASVLATD
                     AYYRGTYAFVYAPARFPGLASLDDARLRSARIGIQVVGIDATPTPAARALATRGIVDN
                     VVGFPVMGETASAARIVDAIAEGTLDIGIVWSPQPGYFIARRHLALGVVPIRHDGVDP
                     AFDFAIAMGVRRDDIALRRELNESLARLAPKIDAVLHDYHVTRIDARERAVQ"
     gene            complement(8192..9913)
                     /locus_tag="VFO53_09340"
     CDS             complement(8192..9913)
                     /locus_tag="VFO53_09340"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HET8876518.1"
                     /translation="MAPARAQIPADDPGNWTMPGRDYASTRFSPLDQLDAGNVGKLAL
                     AWTFSTGVLRGHEAAPIVADNTLFIVTPFPNVVYALDLTQPGAPIKWKFSPYPASSSQ
                     GEACCDVVNRGAAYAYGRVFFNTLDDQTIALDAKTGKEVWRVRLGDIAKGETMTMAPL
                     VVNGEVYVGNSGGEYGVRGWLTALAADSGKTLWRAYSTGPDSDVLIGTGFKPFYPQDR
                     GADLGKRSWPGEAWKIGGGTVWGFVSYDPALGLIYYGTGNPGPWNPAQRPGDNKWTSG
                     IFARDASTGQARWFYQTSPHDLHDYDGINESILIDVAVNGATHHALVHPDRNGYLYVM
                     DRASGQVLSATPYVPITTSLGVDVGSGRITWNPGKAPAPGKTVRDICPASPGGKDWQP
                     AAYSPRTHLVYIPHQNLCQDAMTSQVSYIAGTPYVGAELKMKPGPGGKRGLFTAWDPA
                     AAKAAWKIDEDFPVWSGALATAGDLVFYGTMEGWFKAVDATTGKELWKFKTGSGIVGQ
                     PVSYRGPDGKQYVAVLSGVGGWAGAVVAGDLDTRDQSAGGGFAIAMSDLKQKTAKGGM
                     LYVFALP"
     gene            complement(10121..11524)
                     /gene="mnmE"
                     /locus_tag="VFO53_09345"
     CDS             complement(10121..11524)
                     /gene="mnmE"
                     /locus_tag="VFO53_09345"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET8876519.1"
                     /translation="MAPPPPIAAIATPPGRGGIGIVRVSGTDLAALIRGIAGRALPAR
                     TATRLAFRDAQGAPIDEGLALYFPAPHSYTGEDVVEWHGHGGVAVMRLLLARCVELGA
                     RLAEPGEFTKRAFLNGKLDLAQAESVADVIEASTATAVRAAARSLSGEFSREVHALRD
                     ALIDLRMYAEATLDFPDEDLDLLREGDVAQRLAAIRDRLDRVLLRARSGAILRSGMTV
                     VLVGAPNVGKSSLLNRLVGEDAAIVTAIPGTTRDTVERQIEIGGIPLAIVDTAGLRDT
                     QDEVERQGMARTRGAIARADVALLLVDARDDRTDPFATAHGVDALAAELPATLPRIVV
                     HNKCDLAFAAPHVERYAETIEASPGNAGPATHVWLCALSGEGVALLEGEMLRVAGVTH
                     GLEDAFFARARQLAALDEASGHLRRAADHLETAQPSLELLAEDLRCAQIAFSSITGEF
                     TADDLLGEIFSRFCIGK"
     gene            complement(11562..13247)
                     /gene="yidC"
                     /locus_tag="VFO53_09350"
     CDS             complement(11562..13247)
                     /gene="yidC"
                     /locus_tag="VFO53_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET8876520.1"
                     /translation="MDTQRLILWLVFSFSGLLLWQAWEREHAPVPVPTAKPSQVAPAA
                     TPGAPAPATSAPATSTTAGAPPASASAPTGKIIEIRTDLYAADIDTVGGTITRIALEK
                     HRAAEDTSKPYLALQRNAERTFVAQSGLIGEGFPNHRTEYEPLPGPRTLAPGQDQLQL
                     RLQTTAANGDKVVQVLTFHRGTYVIDVAFDVTNNGAAPITPEAYFQLMRDTKKVGVQS
                     SMAPASFVGPVVYDDEDKFKKVDFGEIDKEAADPTRKPAYTHATDNGWIGMIEHYFVA
                     AWLPPDHAKRQFYTTKLDNGLYTAGVRYGDGAIAPGATGVVSSRLYVGPQDQDTLAKL
                     AKGLDLVVDYGIFTIIAEPLFLLLKWLHSLIGNWGFAIIVMTILIKGAFFPLNAASAR
                     SMAKMKIVAPKMKSLQEQYANDKQQLQVKMMELYRQEKINPLGGCLPIVVQIPVFIAL
                     YWVLLSAVELRHAPWIGWIHDLSAPDPWFILPVVYAVTAWLQVKLSPTPISDPVQAKV
                     MQFMPVAFSIMFLFFPSGLVLYWLVNNSLQILQQWHMNRMLQKEATLAAAKRR"
     gene            complement(13293..13502)
                     /gene="yidD"
                     /locus_tag="VFO53_09355"
     CDS             complement(13293..13502)
                     /gene="yidD"
                     /locus_tag="VFO53_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET8876521.1"
                     /translation="MKRMLLAALAGYRYLLRPMLGANCRFHPSCSDYATEAIERHGAL
                     RGAGLALRRIARCHPWHPGGYDPVP"
     gene            complement(13499..13867)
                     /gene="rnpA"
                     /locus_tag="VFO53_09360"
     CDS             complement(13499..13867)
                     /gene="rnpA"
                     /locus_tag="VFO53_09360"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET8876522.1"
                     /translation="MTHVPTPAPAGRAARYRLRGAGAFATVFKTGARHDGQFLQLIAT
                     PAAQSPGRVGYVIGRKVMRRAVDRNRLRRRLREIVRAARPAVLAFDIVLRVKRIVLRA
                     DIPAASAESRHLLARLVLPQ"
     gene            complement(13864..13998)
                     /gene="rpmH"
                     /locus_tag="VFO53_09365"
     CDS             complement(13864..13998)
                     /gene="rpmH"
                     /locus_tag="VFO53_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET8876523.1"
                     /translation="MKRTYQPSKIRRARTHGFRARMKTVGGRKVLSARRAKGRARLAV
                     "
BASE COUNT         2443 a         4751 c         4815 g         2173 t
ORIGIN      
        1 gaagtaagcg cttactttaa agcttccggg ccagccgatc gcgctcgcgc atcaaccggt
       61 cgggccgctt ttcgccgagt cggtcgaaca gcgcgttgtg cgaatcgatc tcacgttgcc
      121 actgggtcgg atcgacgccc atgatcgccg agaagcgcga tgcgccgaag tcgagccccg
      181 accagtcgag gtgatcgtat tcggggacga gcccgagcga ggactcggtg gcgccggctt
      241 cacccgcgca gcgctcgaag atccatttca gcacccgcat gttctggccg aaccccggcc
      301 acacgaagcg gccttccccg tccttgcgaa accagttgac gctgaacatg cgcggagggt
      361 ggcttaccgc ccgccccatt tcgagccagt gccggaagta gtcgcccatg tggtagccgc
      421 agaacggcag catggcgaac ggatcacgcc gcacttcgcc caccttgccg gtggcggcgg
      481 cggtcgtctc cgagcccatc gtcgccgcct tgtagacgcc ttcgtcccac gtgcgcgcct
      541 cggtcaccag cggcaccgtg tccgagcggc gcgcgccgaa gacgagcgcc gccagcggaa
      601 cgcccgccgg gttgttccat tcggggtcga gcgacggaca ctgcgtggcc gacacggtga
      661 agcgcgcgtt cgggtgcgcc gccggccggc cacacccggg ctcccacgca ttgccgcgcc
      721 agtcgacgag atgcgcgggc ggtgttttcg tcatcccctc ccaccagacg tcgttgtcgt
      781 cggtcaacgc cacgttggtg aagatcacgt tcgcgcgcag cgtttccatc gcgttcggat
      841 tcgacgcgta ggacgtgccc ggcgccacgc cgaagtagcc ggcctcggga ttgatggcgt
      901 gaaacgtgcc gtcggccgcc ggcttgatcc atgcaatgtc gtcgccgatg gtcgtcaccc
      961 gccagccctc gagccccgcc ggcggaatca gcattgcgaa gttcgtcttg ccgcaggaac
     1021 tcgggaacgc cgccgcgacg taccgcttca cgccggcagg ggacgtgacg ccgaggatca
     1081 gcatgtgctc ggcgagccag ccctcgtcgc gtcccatcac cgaggcaatg cgcagcgcga
     1141 ggcacttctt gcccagcagc gcgttgccgc cgtagccgga tccgaagctc catatttcgc
     1201 gcacctcggg gaaatgcacg atgtatttgt tcgccggatt gcacggccag gcgacgtcct
     1261 gcgcacccgg cgccagtggc acgcccaccg aatggacgca cggaatgaac gaaccgtcct
     1321 cgcccagcac gtcgtgcacg cgtgcgccca tccgcgtcat catccgcatg ttggccacga
     1381 cataggggct gtcggtgatc tcgacgccga tctgcgcgat cgggcttccg agcggtccca
     1441 tcgagaacgg cacgacgtac atcgtccgtc cgcgcatgca cccttcgaaa aggccgcgca
     1501 gcgtgcgccg catcttcgcg ggctcgaccc agttgttcgt ggggcccgcg tcttcctcgc
     1561 gctcgctgca gatgaacgtg cgatcctcga cgcgcgcaac gtccgacggg tcggagcggg
     1621 caagaaagcc gttcggtcgc ctggcggggt cgagacggtg cagcgtgccg gcggcgacca
     1681 tgcgctcgca tgacgacacg tattcctcct cgctgccatc gcaccagacg accgtttcgg
     1741 gggccgtcag cgacgcaatt tgcgcgaccc attcgacgag ccgtcggtga cgcacgccgg
     1801 acggcgcgtg taccgacgcc gcgagcgcgg atagcgattc ggaattcatg atgtcaccgc
     1861 caaggattca ttggacgaaa cgggaccgaa aactctagca cgcatgatgc gctcacccaa
     1921 ggtgcactgc agtatgccgc ggaccacgtt tcgacgtggt tcgcgcacgc tcgttcatcc
     1981 gggccggcgc cgaacggcgc tcggcgaaag ccatgtgccg acgatcgaca acgcgacgac
     2041 gacataggcg aggccgcacg gcacccacat gatgaggccg ccgagttgct gatcctcgag
     2101 cggcgtgagc ccgaacgcac gcgggcccgc gtcgtgcacg taccacggcg acggcgcaaa
     2161 ggcgagaagc gcaccgagcg cactggtgtg aagcatggtt gcgaacaggc acgcgaccga
     2221 gaccgcgccc ggcgtacgca tcgcgccacc gaacatcgcc caccagaacg ccagcgccga
     2281 cgcgaagaag cagacatgct ggaacacgtg cagtggcgtg ctcgccagtg cagcgaggaa
     2341 gagcggcggc agatgccaga tccatagcgc gagcgcatgg acgcaggttg cgaagaccgg
     2401 tgcgcccgtc gagcgcacga tccgccgcac gagcgtcgag cgcgtgaacg ccaccatcgc
     2461 gcgccgcgtg ccacccggca acgcccatgc ggaagcctcc agcggccgcg ccagcacgaa
     2521 aagcggcgcg gcaatcacca tcagcatctc gtgctcgatc atgtgcgtcg caaacgatcg
     2581 gccggcgagc gcgtcgatcg gcggcaggag tgccgcggcg agtgtcgcga tgccgagccc
     2641 gaagcgcgcg acatcgacgg cgcgaatgcc acgacccacc ccggccttgc gccagaggcg
     2701 cgtgacacca cgcgcgtaga ggacggcgct cgccaggaga gcactcgcga gcacggcatc
     2761 gatatgcaca tgcaagtgag tgacggcgcc cgcgacgtgc gccatgcagt cggttgcgac
     2821 gagcgcgggc agcgcgccgc cgatcagtag acgcaggcgc tcagcagcca actcaccgcc
     2881 cacatcgcca ggatgacgag caccgacaac gcggcggcgc cggtggcgag cccggcgagg
     2941 aaatgacgac gcgcaagcgt ttcattgtcg gccttcatcg cggacgaccg gcgccagctc
     3001 tgccacgcgg cgatcgtgcc tgtcgccgca acgagcagaa acggcacgtg cacggcgtgc
     3061 atggcgacgg tgttctggtg gtcgcacgac cagacgctca gcacgtacga agcgctctga
     3121 tcggcaagcg ccagcagcgg tacggccagc aacgcgaacc agacgcgcat gatcagaatc
     3181 gtgggcccca atagacaacg gcgtagatcg gaatccacgt cagcacgatg aaccaccagt
     3241 agccggcgtt ctcggcgacg tcgacgaagc gcttcccatc gaccgggcgc ttgaacatga
     3301 ccgccgccac gacgagcgaa tcggcgtagt tcgtgacgag attgaatgta tgcaggccga
     3361 gcagaaccca tacggccgac ccataggcgc tcgcgtccca gcgcacgttc agcgcatcga
     3421 attcgagcca gcgcacgccg agcagcgcga gcccgtagat cacgaacacg atcaaccaga
     3481 ggcgaaccct ggcgaggtcg tggtcgtcgg ccgccttgtt cgccatcagg ttgggcacga
     3541 tgctgaggca cgcgagcccg agattcaccg tgccccacaa cagacccggc ggtccgcctt
     3601 cgttgggcca cagggggctg cggctcttca ggtagaagta cacgacaatc atcatggcga
     3661 agcccatgcc ctcgatcgcc gacatccccg cggttcccca ccacatgagg ctgcgcgtgc
     3721 cgaagccgta gctcggcagg ccggagacgt cgagcgcatc gccgcggcgc accgccggaa
     3781 tgctcgcggg cgtcgactgg cgccgtcttc ggatcgcctt gtcctcgcgt gccgggtcga
     3841 cccggtcgtc gatgcttgcc gatctcgcca tgctcatggc cgccgctgca gcaacaattc
     3901 ctcggcggtc tccttgcgct tcggccaata ccaggcaatg aaggcaatcg ccggggggat
     3961 caggccgtag tagaggcccg acggggtgac gttcgaccag aacagccaga tgtcgaccgt
     4021 caccgccgcg atgaagggcc agacggtcgg cgacggatac gagtagcgca catcgggaag
     4081 cgcatcgagg accgtcgtga cgaggccctc gcgcgtctgc gccgacagtc cgctcacgtg
     4141 cgtgggctcg tcggcctcgc gtgcccacaa cggctcgcgc gatgcgacaa cgggcagcgc
     4201 cacgaagttg aacgcgggcg gcggcgagtg cgtcgcccac tcgagggtcg aagcggccca
     4261 cggattgtcg ccggcaagcc tgcccttcgc gacactgcgc gcgaagttca cgacgaacag
     4321 cagcacgctc acggcgatga tcgtcgcgcc gatcgtcgcg atcaggttga gcgttcccca
     4381 gccggtctcg gcgccatacg tgtacacgcg tcgcggcatc ccttcgaggc cgagaatgtg
     4441 catcgggaag aacgcgacgt tgaagccgac gaagaacaac cagaactgcc agcgtccgag
     4501 gcgctcgtcg agcatccgcc cggtgaactt cgggaaccag tagaagaacg cgccgaacag
     4561 cggaaagacg gcgccgccga gcagcacgta atgcaggtgc gcgacgacga aatagctgtc
     4621 gtggacctgc aggtcgaacg gcaccgcgcc gaccatgatg ccggtgagtc cgccgagcac
     4681 caggatggcg aagaagccga gcacgaacag cagcggcacg cgaacattga gccgtcccgt
     4741 ccacagcgtc gcgatccagc agaatatctg tgcggcggtg ggaatcgcga tcatgaagct
     4801 catcgccgtg aagaagctct tgccgagctc gggcaggttc gtcgcgaaca tgtggtgcac
     4861 ccagaggccg aacgcgagga acccggtggc gatcagcgac agcacgacgg ctgcatggcc
     4921 gaacagcggc cggcgcgcga acgtcgggat gatcatcgaa acgaacccga gcgccgggat
     4981 gaagatgaag tacacctcgg gatggccgaa aaaccagaac aggtgctgcc agatcagcac
     5041 gtcaccgcct tcggcgggat tgaagaaatg cgtgccgaac gtccggtcga actgcgcgag
     5101 gttcgcggcg agcatcaccg acggcatcgc gaagatgatc atcatcgacg cgacgagcga
     5161 cgaccatacg aagaccggca tccggcgcag cgacatgccc ggtgcgcgca tcttgaggat
     5221 cacggtcgcg aggtcgaccg ccaccatcag gcccatcgcc tcggtcagat tgatcaggtt
     5281 gttccagacg tcggcgcgct tgccgatgcc gtactgggga cctgcgagcg gaacatacga
     5341 gaaccagccg gcgtcggcgc cggtattgat cgcgaagccg gtccacagcg acagcgcgcc
     5401 gagcagatac agccagtacg cgcaggcggt catccgcgga aacgccgtgt tgcgcgtgcc
     5461 gaccatcagc ggcaccaggt agacgcccat tccctgcatg atcggcacgg cgaagatgaa
     5521 catcatcgtc gtgccgtgca tcgtgaagag ctggtcgtag aggtcggggc cgacgacgtg
     5581 gttgtcggga aaggcgagtt gcgtgcgcat caccgcggca agaagtcccg ccgcgcagaa
     5641 gaagacaaag gcggtgacga cgaagcgcac gccgatgcgc ttgtgatcgt tcgtcttgag
     5701 ccagccgatg aatcccggct cgtcgcgcca cgcatgctcg agcgccagcg cggccttcga
     5761 tccgaagccc gcgccgtcga gcagcgtctc gccccgtccg acggcggcgt ctgcctgttc
     5821 gcggagcgca tgctgcgacc gtgtggctcc gggtgctgaa agcgagccgg cgtcggcggt
     5881 catttcagcg acccgagata cgcattgagc gctgccagct cctcgggcgc aaaactttgc
     5941 gccggcatgt tggcacccgg cttgaactgc tgcggatcga ggatccatgc ggcgcgcatg
     6001 ctcggcgtgt tgtcgagcgt gccggcgccg atcgtcatgc ggcttgcgac atgcgtgagg
     6061 tcgggtgcat tgcgcgcctg cgctgtcgtg ccgccgatcg catgacacat cgcgcagctt
     6121 cggctgacga aaagatcgcg gccgcgcact tgcgaggcgg tgaccggtgc ggcggcgggc
     6181 tcgcgctgcc gcgccgccca ctgcgcgtag acgtcgggcg catcggcgac gacatacagc
     6241 accatgtgcg catgctgata gccgcaatac tcggcgcact ggccgcgata gattccaggc
     6301 cggtcggcgc ggaacgacac ggttgcgtca cgtcccggaa tgagatcctt cttgccgtgc
     6361 aggctcggca cccagaagct gtggatcacg tcggcgcccg cgaggctcac gatgaccggc
     6421 cgcccgacgg gcacgtgcag ctcgttggcg gtcgtgaaga tccgcgacgc atcggcgtcg
     6481 tcgtagcgcg ccgcccacca ccactggtag ccggtgagct cgatgtgcag cgcatccgcg
     6541 agcggcaatc gggcgatcgc gcggtcggtg agcacgctcg ccgcaagcag caccagcagc
     6601 aggacgatcg agacggcgat ggcaacccac accgttcgca ccacgcgggg ctcgcgctgc
     6661 cacagcgaag agacgtcggg cgcagcatcc tgcgcgacgc gcggcgcacg ccgcagcgcc
     6721 agcgcgagcg ccattatgac ggcgacgaac acgagcgtgc agatcggcaa cagccaccac
     6781 cacagatcga cgatcgcccg cgcctgcgcg ccggccggaa gctgcacgtc gtgcagtccg
     6841 ccgatcatgg gcgctcggac gcgcccgacg gcgcggcgac gaccggtgtc tgctgctccg
     6901 tctggctctc cggtttcctg acctgcatcg cgtcggcacg attgggagcg gcactgctgg
     6961 aggcgagtcc cgacagcgag cgcacgtacg cgacgagctc ccagacttcg ttgtccggaa
     7021 catggccgcc gaacgacggc atgccgttcg gccggccctc gacgatcgtc gcgtagatgt
     7081 tcgcgggatc gctgccgtag acccacacgt tgtccatcag cgccggtccc ttgtcgccgc
     7141 cgccgttcgc gtggcagccg gtgcagttgt accagccgaa gaggcgcttg ccctccgaca
     7201 aggcgtacgc gttgtcctcg tacttccgct cgaccgatgt gttcgtggcg atcgcgcccg
     7261 gctggatcgc gctcatgcgg atgggaggcg gcgagacggc cttcgtcggc ggctcgaagt
     7321 cgcggctctc gcgctggcag ccggcgagcg cgagcgccgc gagcgccgtt attgcgaccg
     7381 cgtgcgccct gtcgcgtttc attgcactgc ccgttcgcgc gcgtcgatgc gcgtcacgtg
     7441 gtagtcgtgc agcacggcgt cgattttcgg cgcaagccgc gcgagcgatt cgttcagttc
     7501 acggcgtagc gcgatgtcgt cgcggcgcac ccccatcgcg atcgcaaaat cgaacgcggg
     7561 atcgacgcca tcgtgacgaa tgggcacgac gccgagcgcc agatgacgcc tggcgatgaa
     7621 gtagccgggc tgtggcgacc agacgatgcc gatgtcgagc gtgccctcgg caatggcgtc
     7681 cacgatgcgc gccgcggatg ccgtttcgcc catcaccgga aagccgacca cgttgtccac
     7741 gatgccgcgt gttgcgagtg cgcgcgcggc cggtgtcggt gtggcgtcga ttccgacgac
     7801 ctggatgccg atgcgtgccg agcgaagccg cgcgtcgtcg agactcgcga gcccgggaaa
     7861 gcgcgccggc gcgtacacga acgcgtaggt gccacggtag tacgcgtcgg tggcgagcac
     7921 gctcgcatcg gcctgcgcga tcccgggaat cacgtcgcag cgatgctgct gcaacgtttt
     7981 tcgcgcgtag ccgcgccatt gcggaagcca gtagtagcgg aggtcggcgc gaagatcgtg
     8041 cgcgaccagg ctcgcgatcc ggttttcgaa gccgcgttcg tcgcgatccg aatacggcaa
     8101 gttgccggga tcggcgcaca cgcgaagctc gcgtgcggcg gtgggggccg aaacgaggag
     8161 cgccagtgcg cagagtgcga gcgcgcggat ttcaaggcag cgcgaagaca tagagcatgc
     8221 cgcccttcgc cgtcttctgc ttgagatcgg acatcgcgat cgcgaagccg ccgccggcac
     8281 tctggtcgcg ggtgtcgagg tcgcccgcca ccaccgcacc cgcccagccg ccaacgcccg
     8341 agagcaccgc gacgtactgc ttgccgtcgg ggccgcgata cgacacgggc tgacccacga
     8401 ttcctgatcc ggtcttgaat ttccacaact ccttgcccgt cgttgcgtcg acggccttga
     8461 accagccctc catcgtgccg tagaacacga ggtcgcccgc cgtggcgagc gccccgctcc
     8521 acacgggaaa gtcctcgtcg atcttccagg cggctttcgc agccgcggga tcccacgccg
     8581 tgaagagtcc gcgcttgccg ccggggcccg gcttcatctt gagctcggcg ccgacgtacg
     8641 gcgtgcccgc gatgtagctg acctgcgagg tcatcgcatc ctggcagagg ttctggtgcg
     8701 ggatgtagac gaggtgcgtg cgcggcgaat acgcggccgg ctgccagtcc tttccgccgg
     8761 gcgatgccgg gcagatgtcc cgcaccgtct tccccggtgc gggcgccttc ccgggattcc
     8821 acgtgatgcg gccgctcccg acgtcgacgc cgagcgacgt ggtgatcggc acgtacggcg
     8881 tggccgacag cacctggccg ctcgcccgat ccatcacgta gaggtagccg ttgcgatccg
     8941 ggtgcacgag cgcgtgatgg gtcgcgccgt tgaccgcgac gtcgatcagg atgctctcgt
     9001 tgatgccgtc gtagtcgtgc agatcgtgcg ggctcgtctg gtagaaccag cgcgcctgcc
     9061 cggtgctcgc atcgcgcgcg aagatgccgg aagtccactt gttgtcgccc ggccgctgcg
     9121 ccggattcca cggtccggga ttgccggtcc cgtaatagat gagcccgagc gccgggtcgt
     9181 acgacacgaa accccacacg gtgccgccgc cgatcttcca cgcttcaccg ggccacgagc
     9241 gcttgccgag atcggcgccg cgatcctgcg gatagaacgg cttgaaaccg gtcccgatca
     9301 gcacgtcgct gtccggcccg gtgctgtacg cgcgccacag cgtcttgccg ctgtcggcgg
     9361 cgagcgccgt cagccagccg cgcacgccgt attcgccgcc actgttgccg acgtagacct
     9421 cgccgttgac gacgagcggc gccatcgtca tcgtttcgcc cttcgcgatg tcgccaagcc
     9481 gcacccgcca gacttccttg ccggtctttg cgtcgagtgc gatcgtctgg tcgtcgagcg
     9541 tgttgaagaa gacgcgcccg taggcatagg cagcgccgcg gttgacgacg tcgcagcacg
     9601 cttcgccctg cgacgacgac gcagggtacg gagagaactt ccacttgatc ggcgcgcccg
     9661 gttgcgtgag gtcgagcgcg taaacgacgt tcgggaatgg cgtgacgatg aagagggtgt
     9721 tgtcggcgac gatcggcgcc gcctcgtggc cgcgcaggac gccggtcgag aacgtccacg
     9781 ccagcgcgag cttgccgacg ttgcccgcgt cgagctggtc gagcgggctg aagcgggtcg
     9841 acgcgtagtc gcggccgggc atcgtccagt tgcccggatc gtcggccggg atctgtgcgc
     9901 gcgccggcgc catgacgaca gcggcgagca cgagggcaag acgaacgcgg ttcacaaggt
     9961 cgctccttgc tgtgccgggc aaagcgcaag gagcgtacca agcaaaaagc tatttcgctc
    10021 agttacttag atcgacattt tcgctgcctt cagcaataat tcctcgcgcc tggaggaaca
    10081 tttttccggc ggccgccgcg cggagcgcgg cgcatgaacg ctacttgccg atgcagaagc
    10141 gggaaaagat ttcgccgagc agatcgtcgg ccgtgaactc gccggtgatc gatgagaatg
    10201 cgatctgtgc gcagcgcagg tcctcggcca gcagctcgag cgacggctgc gcggtctcga
    10261 gatgatcggc tgcccgccgc aggtgcccgc ttgcctcgtc gagggccgca agctgccgcg
    10321 cacgcgcgaa gaacgcgtct tcgaggccgt gcgtcacgcc ggcgacgcgc agcatttccc
    10381 cttcgagcag cgcgacgcct tcaccgctca acgcgcatag ccagacgtgc gtggccgggc
    10441 cggcgttgcc cggggacgcc tcgatcgttt cggcgtaccg ctcgacgtgc ggcgcggcaa
    10501 acgcaagatc gcatttgttg tgcacgacga tacgcggcaa cgtcgcgggc aactcggctg
    10561 cgagcgcgtc gacgccgtgc gccgtcgcga agggatcggt gcgatcgtca cgcgcatcga
    10621 cgagcagcag cgcaacgtcg gcgcgcgcaa tcgcgccgcg cgtacgcgcc atgccttgtc
    10681 gctcgacctc gtcttgcgtg tcgcgcaggc cggccgtgtc gacgatggcg agcggaatgc
    10741 cgccgatctc gatctggcgc tcgacggtgt cgcgtgtggt gcccgggatc gcggtgacga
    10801 tcgccgcgtc ctcgccgacg aggcggttca gcaggctcga cttgccgacg ttcggcgcgc
    10861 cgacgagcac gaccgtcatg ccgctgcgca ggatcgcccc gcttcgggca cgcagcagca
    10921 cgcggtcgag ccggtcgcgg attgcagcga ggcgctgcgc gacatcgcct tcgcgcagca
    10981 ggtcgagatc ctcgtccggg aagtcgagcg tcgcttcggc atacatgcgc aggtcgatga
    11041 gcgcatcgcg cagcgcgtgc acctcgcgcg aaaactcgcc gctaaggctg cgtgcggcgg
    11101 cgcgcaccgc ggtggctgtc gatgcctcga tgacgtcggc cacgctctcg gcctgggcga
    11161 ggtcgagctt gccgttgagg aatgcccgct tcgtgaattc gccgggttcg gccagtcgcg
    11221 cgccgagctc gacgcagcgc gcgagcagca gccgcatcac ggcgacgccg ccgtggccat
    11281 gccattcgac gacatcctcc ccggtgtacg agtgcggcgc cggaaaatac agcgcgaggc
    11341 cctcgtcgat cggcgcgccc tgcgcatcgc gaaaggcgag tcgcgttgcc gtccgtgcgg
    11401 gcagcgcgcg gcccgcgatg ccgcggatca acgcggcgag atcggtgccg gaaacgcgga
    11461 cgatgccgat gccgccgcgc ccgggcggcg tggcgatcgc ggcaatcggc gggggcggcg
    11521 ccatgcgcgc aagcctgtcg gttcgaccac gccccgcacg ctcagcggcg tttggccgcg
    11581 gcgagcgtgg cctccttctg cagcatccgg ttcatgtgcc actgctgcag gatctgcagc
    11641 gaattgttca cgagccagta cagcacgagg cccgacggaa agaacaggaa catgatcgag
    11701 aacgcgaccg gcatgaactg catcaccttc gcctgcaccg gatcggagat cggcgtcggc
    11761 gaaagcttca cctgcagcca cgcggtgacg gcatacacga cggggaggat gaaccaggga
    11821 tcgggcgccg acaggtcgtg gatccagccg atccacggcg catggcgcag ctcgaccgcg
    11881 gacagcagca cccagtagag cgcgatgaac accgggatct gcacgacgat cggcaggcat
    11941 ccgccgagcg ggttgatctt ctcctgccgg tacagctcca tcatcttcac ctggagctgc
    12001 tgcttgtcgt tcgcgtactg ctcctggagc gacttcatct tcggcgcgac gatcttcatt
    12061 ttcgccatcg agcgtgcgga cgcggcgttg agcgggaaga acgcgccctt gatgaggatc
    12121 gtcatcacga tgatcgcgaa gccccagttc ccgatcagcg agtgcagcca cttgagcagc
    12181 aggaacaagg gctcggcgat gatggtgaag atgccgtagt cgacgacgag gtcgaggccc
    12241 ttcgcgagct tggcgagcgt gtcctggtcc tgggggccga cgtagagccg cgacgaaacg
    12301 acaccggtcg cgcccggcgc aatggccccg tcgccatagc gaacgccggc cgtgtagagc
    12361 ccgttgtcga gcttcgtcgt gtagaactgg cgcttcgcat ggtcgggcgg cagccacgcg
    12421 gcgacgaagt aatgctcgat catcccgatc cagccgttgt cggtggcatg cgtgtacgcg
    12481 ggctttcgcg tcgggtcggc ggcctccttg tcgatctcgc cgaagtcgac cttcttgaac
    12541 ttgtcctcgt cgtcgtagac gaccgggccg acgaacgacg cgggcgccat cgagctttgc
    12601 acgccaacct tcttcgtgtc gcgcatcagc tggaagtacg cctcgggcgt gatcggcgct
    12661 gcgccgttgt tcgtcacgtc gaatgcgacg tcgatcacgt acgttccgcg gtggaacgtc
    12721 agcacctgca cgaccttgtc gccgttggcc gcggtcgtct gcagcctgag ctgcaactgg
    12781 tcctgccccg gcgcgagcgt gcgcgggcca ggcagcggct cgtactcggt gcggtgattc
    12841 ggaaagccct cgccgatcag gcccgactgc gcgacgaacg tcctctcggc gttgcgttgc
    12901 aacgcgaggt acggcttcga ggtgtcctcc gcggcacggt gcttctcgag cgcgatgcgc
    12961 gtgatcgtcc cgccgacggt gtcgatgtcg gcggcgtaga gatcggtgcg gatctcgatg
    13021 atcttcccgg tcggtgccga ggcgctcgcc ggcggcgcgc cggcggtcgt gctcgttgca
    13081 ggcgcgctcg tcgctggcgc aggcgcgccc ggcgtcgcgg cgggcgcgac ctgcgacggc
    13141 ttcgcggtcg ggaccggcac gggcgcgtgc tcgcgctccc atgcctgcca caacagcagc
    13201 ccggagaacg aaaagacgag ccagaggatc aggcgttgcg tatccatgag gggtctgtca
    13261 gaggttgcgg ccgctcgcga agcgcgacgg cgttacggca cgggatcgta accgcccgga
    13321 tgccagggat ggcagcgcgc gatgcgccgc agcgcaagcc cggcgccgcg cagcgcaccg
    13381 tggcgttcga tcgcctcggt tgcatagtcc gagcaactcg ggtggaagcg gcagttggcg
    13441 cccagcatcg gccgcagcag ataccggtag ccggcaaggg ctgcgagcag catccgcttc
    13501 attgcggtaa aacaaggcgc gcaagcagat gccgcgactc ggcgctggcc gccgggatgt
    13561 cggcgcgcag aacgatgcgc ttgacgcgca gcacgatgtc gaacgcgaga acggcgggac
    13621 gcgccgcgcg cacgatttcg cgcaagcgcc gccgcagtcg atttcgatcg acggcccgcc
    13681 gcatcacctt gcgcccgatg acatagccga cgcggccggg cgattgcgcg gccggggtgg
    13741 cgatcagttg caggaactgc ccgtcatgac gggcaccggt cttgaatacc gtcgcgaacg
    13801 cgcccgcgcc gcgaaggcga taccgggccg cgcgtccggc cggggccgga gtcggaacgt
    13861 gcgtcagacg gcaagccggg cacggccctt ggcacgccgg gccgacagca ccttgcggcc
    13921 gccgacggtt ttcatgcggg cgcgaaatcc gtgcgttcgc gcacgacgga ttttcgatgg
    13981 ctgataagtg cgtttcattg cgtacctcgt gagtgattct gcggcgcgcg gctcgcgttg
    14041 cgcggcagac gggcctgcga tggccggcgc gggcggacga tgcgcggccc tgccaagggc
    14101 ggtgcttccc gaaaagcctt acattagagc gcgttacgcc tcgattgtca aacaagcgat
    14161 ccccgtgcgc gatgcgtgcg gc
//
LOCUS       DASSVX010000218         9273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_359073, whole genome shotgun sequence.
ACCESSION   DASSVX010000218 DASSVX010000000
VERSION     DASSVX010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359073"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1797)
                     /locus_tag="VFO53_09370"
     CDS             complement(<1..1797)
                     /locus_tag="VFO53_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HET8876524.1"
                     /translation="MTHDGYLRHPTIRNGVIAFICDDDVWRADANGGVARRLTAGLGE
                     PSTPALSPDGKWIAFVGRDEQHPEVYLMPSEGGPVRRMTWLGPDVMVRGWTPEGRILF
                     VTTYGQPFFRNYRAFTLPIEGGMPQLIPLGQVNHLAYGPGDAKVIGRNTADPARWKRY
                     RGGTAGYLWIDAAGNGDFRRMRELAGNITSPMWIGERIYYLSDAEGVGNLYSCRPDGS
                     DRRRHTDHDEFYARHAQTDGRHVVYQCGADIWRFDPAQDRTARVPIDVPASRTQAARR
                     FVDAAENLAGFQLHPSGHSLAIEARGKLFTFALWEGAVRQHGVADGVRYRYGQWLADG
                     ETLVAISDESREERLVAFHAGDVKSFPWDIGRVIAMRAAPTGQRVAIANHRHEVIVAD
                     LDSNTFAVIDRSDSGRTEDLAWSPDGGWLAYTFWTSPRHSAIKLHDVAKSSGTLVTDP
                     EFRDYSPAFDPAGKYLYFLSLRTFDPVYDNVQFELSFPRAARPYLIALKADERPPFEP
                     EPAGMQDNDAKTARNARKEAPPAVRVDLDGIARRVAAFPVPEGRFGQVAGVAGDKVVW
                     TLMPVEGAHGRGGHKDLPGRLELFDFRTMKSEL"
     gene            complement(1924..2673)
                     /locus_tag="VFO53_09375"
     CDS             complement(1924..2673)
                     /locus_tag="VFO53_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8876525.1"
                     /translation="MHKRSGSDEARLFRDSPMPRYAQLADVFRQRIARNHWPPGTKLP
                     TLEALMREFDVARVTVRQAMELLGRDGLVSAERGRGTFVTAQPVRDRRLVLETSLQAL
                     ADVYRDDRPNLTLIEEASAAPRLFPEDGKPAPRYRFMRRVHSRNGEAYCVISIYLDER
                     VFRMAPRRFRRETVVPLLLDLPHVEIARAWQTLEIGTADVLVARHLGIHVNAPVAEVR
                     RVCRAKDETVLYLGEVTYRGDYIHLAMDLKP"
     gene            2800..3555
                     /locus_tag="VFO53_09380"
     CDS             2800..3555
                     /locus_tag="VFO53_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876526.1"
                     /translation="MARRPALIALPWLIVVGIWYAIAYSGLINPSLVPTPHRVAWRFW
                     ELLTTARLPLDILMSTGRVVVGVALGITLAVPVGFVLGWYPNVRAFIDPLMNFFRALP
                     PIALIPLVIVYFGIGEIAKIVVLFYASFFAGVIVMYEGIAQISPIYIRVSKTLGASDV
                     EIFRKVIVPLTVPHLLTALRVALGVAWATLVASELIAAQQGLGALIQNASSFFQLDII
                     YVGIICIGLIALAMDLVLRAIARRLVAWQERIA"
     gene            3662..4486
                     /locus_tag="VFO53_09385"
     CDS             3662..4486
                     /locus_tag="VFO53_09385"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876527.1"
                     /translation="MSATPRIVFDHVSMEFQSKQGPLRVVDDVSYAIGDGDFVAVIGP
                     SGCGKTTMMNMVAGFVVPTHGRVLLDGRPVSGPGPDRGVIFQEYGVFPWLTVRQNIAF
                     GLTLAANRAHAPDRDAICARYMKLMGLSDFADAWPKMLSGGMRQRLALARAYAVRPQF
                     LLMDEPFGALDAQTRNAMQDLLLEVLATEGKTVMLITHSVEEAIYLASRIVVMSARPT
                     RIREEITVPFPYPRSESLHETREFNELRSHIRELVMKEYAAQAQLAGSATTFAAPM"
     gene            4600..5601
                     /locus_tag="VFO53_09390"
     CDS             4600..5601
                     /locus_tag="VFO53_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876528.1"
                     /translation="MNRRQFLQTTAAVGIGAGAAVLGAPAIAQSRAKVKVGYLHTLAV
                     DGQIWTGIDRGSFAKHNVELDLVQFTTGLELFQAMIGGSIEMLATGAVISNFPARGQG
                     KMFLVNDVEFATAQLWVRGDQIKSFADLKGKKISTTTGTTAHVFLDTALRANNVDPKD
                     VEIVNQRMSEAVTSFISGAVPAVALWVPFNIPVREKVPNAKMLADASAYYPKAAIVGG
                     WAVRDDYYAKNRAECANIIRGWAEANDYMIANADAALDALQKHHYQQVPLSDLKEQYK
                     AQKMFTSKDWKKLYADGTVTNWLQQVTDFFVRFGGIQNPVPASTYFDPKLYLDTIKA"
     gene            5606..7219
                     /locus_tag="VFO53_09395"
     CDS             5606..7219
                     /locus_tag="VFO53_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET8876529.1"
                     /translation="MAEASDTFDYVIVGAGSAGCILANRLSADATTRVLLLEAGGSDR
                     NFWIRLPVGYFRTIYDPRFARLFDTVPSEGTAGRNVLWPRGRVIGGSSSINGLVYIRG
                     QHEDFDDWAAQGAAGWRYLDVLPDFKRIEHYAGGASAYHGASGELGVCNLRNDHPYCS
                     AWVDAGVESGLPRNPDFNGATTYGVGSYQLSIDNGWRQSAAVAFLRPASRRPNLSILP
                     RALATRVRFEGTRALGVEWQRGGQTHFARAAREVILSAGTIQSPQLLQLSGVGPPALL
                     RGLGIPVVAGLSGVGENLQDHYQARTIVRLRKRVSLNDDVRNPLKLAGMGAEWLFRRS
                     GPLTVGAGQVGGAACTEHATGNRPDVQFNVMPLSVDKPGDPLHPYSGFTAAVWQCHPE
                     SRGRLAIRSTNPADAPGIEPRYLDADRDRKTLVAGIRMLRDIYRQPSFRELFDAEMLP
                     GTDARYDEDLLDFARHAGGTVFHCVGTCRMGDARDDDAVVDPQLRVRGVEALRVIDAS
                     VMPSVTSANTNAASLMIGEHGARIVLQGG"
     gene            complement(7305..8672)
                     /locus_tag="VFO53_09400"
     CDS             complement(7305..8672)
                     /locus_tag="VFO53_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET8876530.1"
                     /translation="MSIQAINPATDEVIATHDEMTPQLVAGIVKSAHGAFVDWRHASY
                     AERAVPMRRAAAILRKNARDFAHLMAREMGKPVRDGVAEAQKCATACDYFAEHAAAFL
                     ARETVPTEARSSFVTFKPLGVVLAVMPWNFPFWQVFRFAAPGLMAGNAAVLKHASNVP
                     GCALAIEDVFRGAGFPENLFRTLMIGSKSVDAVIEDPLVRAVTLTGSGPAGRAIAAKA
                     GQLLKKTVLELGGSDPYLVLEDADLALAADVCTKARLVNSGQSCIAGKRFIVVERVRA
                     EFERRFVKRMAASKMGDPLDESTEIGPQARADLRDTLQRQVDRSIAKGARCMLGGAIP
                     EGPGAWYPPSVLTDVRPGMPAFDEELFGPVAAVVPVRDEAEAVAMANASAFGLGAGVI
                     TRDIARGERIAADLIESGCVFVNDAVRSDPRLPFGGIKESGHGRELASYGIREFVNIK
                     TVYVA"
     gene            8783..>9273
                     /locus_tag="VFO53_09405"
     CDS             8783..>9273
                     /locus_tag="VFO53_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876531.1"
                     /translation="MSAEGPPRGANSAPAGGSAAAKPQAWGDHPKASAAERVTHPERR
                     SLRERMSGMRNIPPFLALVWQTSPSMMLGEAVLRVVRALLPVVTLYVGKLIIDEVVLL
                     TKTPPPGDELRAWLESGALDRIVSLLALEFGLAVLSDVLGRAASLLGQLFGQAQDIVT
                     IVS"
BASE COUNT         1493 a         3113 c         3110 g         1557 t
ORIGIN      
        1 cagctcgctt ttcatcgtcc ggaaatcgaa gagctcgagg cgtcccggca aatccttgtg
       61 gccgccgcgg ccgtgcgccc cctcgaccgg catcagcgtc cagacgacct tgtcgcccgc
      121 aacgccggcg acctggccga agcgcccctc gggcaccgga aacgcggcga cgcgtcgcgc
      181 gatgccgtcg agatcgacgc gtaccgccgg cggcgcttcc ttcctggcat tgcgggccgt
      241 cttcgcgtcg ttgtcctgca tgcccgcggg ctcgggctcg aacggcggtc gctcgtcggc
      301 cttgagcgcg atcaggtagg ggcgtgccgc gcgcggaaac gacagctcga actgcacgtt
      361 gtcgtagacc gggtcgaacg tgcgcaacga caggaaataa agatatttgc ccgcgggatc
      421 gaacgccgga ctgtaatcgc gaaactcggg atcggtgacg agcgtgccgc tcgacttcgc
      481 cacgtcgtgg agcttgatcg ccgaatggcg tgggctcgtc cagaacgtgt acgcgagcca
      541 cccgccgtcg ggcgaccatg cgaggtcctc ggtgcggccg ctgtcgctgc ggtcgatgac
      601 cgcgaatgta ttgctgtcga gatccgcaac gatgacttcg tggcggtggt tcgcgatcgc
      661 cacccgctgc cctgtcggcg ccgcgcgcat cgcaatcacg cgcccgatgt cccacggaaa
      721 cgacttcacg tcgccggcat ggaacgcgac gagacgctcc tcgcggcttt catcgctgat
      781 cgcgacgagc gtctccccgt cggcaagcca ttgaccgtag cgatagcgca caccgtcggc
      841 gacgccgtgc tggcgcacgg cgccttccca cagcgcgaac gtgaaaagct tgccgcgcgc
      901 ctcgatggcg aggctgtggc cggatggatg cagttggaag ccggcgaggt tttccgccgc
      961 gtcgacgaat cggcgcgcgg cctgcgtgcg cgacgccggc acgtcgatgg gcacgcgcgc
     1021 ggtgcgatcc tgcgccggat cgaagcgcca gatgtcggcg ccgcactggt agacgacgtg
     1081 ccggccgtcg gtttgcgcgt ggcgcgcata gaactcgtcg tgatcggtgt gccgccgccg
     1141 gtcggagccg tccggccggc acgaatacag attgccgacg ccttccgcat cggacaggta
     1201 gtagatgcgc tcgccgatcc acatcggcga cgtgatgttg cccgcgagct cgcgcatccg
     1261 gcgaaaatcg ccattgccgg cagcatcgat ccagaggtaa cccgcggtgc cgccgcgata
     1321 gcgcttccag cgcgcaggat cggccgtgtt gcgaccgatc accttcgcgt cgcccgggcc
     1381 ataggcgaga tggtttacct ggccgagcgg gatgagctgc ggcatgccgc cttcgatcgg
     1441 caacgtgaac gcgcggtagt tgcgaaagaa cggctgcccg tacgtcgtca cgaacaggat
     1501 gcgcccttcg ggcgtccatc cgcgaaccat cacgtcggga ccgagccagg tcatccggcg
     1561 caccgggccg ccttcggacg gcatcaggta cacctcggga tgctgctcgt cgcggccgac
     1621 gaaggcgatc cattttccgt cgggcgaaag cgcgggtgtc gacggctcgc ccagccccgc
     1681 ggtgaggcgc cgcgcaacgc cgccgtttgc gtcggcccgc cagacatcgt cgtcgcaaat
     1741 gaacgcgatg acgccgttac gtattgtcgg atgccgcaga tagccgtcgt gcgtcatgat
     1801 ggaactcagg atggggatcg agcccatgat tttgccccat tcatcgcagg cggcgtgcgt
     1861 gcgacggcgc gaatattcgg cttcgacgtc gcttgtgagg cgaacaatcg gaggacgcct
     1921 gtctcagggc ttgaggtcca tcgcgaggtg gatgtaatcg ccgcgatagg tcacctcacc
     1981 gaggtagagc accgtttcgt ccttggcgcg gcacacgcgg cgcacctcgg ccaccggcgc
     2041 gttgacgtga atgccgaggt ggcgtgcgac gagcacatcg gccgtgccga tctcgagcgt
     2101 ctgccacgcg cgagcgatct cgacgtgcgg gagatcgagc agcagcggga ccaccgtctc
     2161 gcgccggaag cgccgcggcg ccatccggaa cacccgctcg tcgagataga tcgagatcac
     2221 gcaataggct tcgccgttgc gcgaatgcac gcggcgcatg aagcggtagc gtggcgcggg
     2281 cttgccatcc tcggggaaga gccgcggcgc ggccgatgcc tcctcgatca gcgtcaggtt
     2341 cggccggtcg tcgcgataga catcggcgag cgcctgcagg ctggtctcga gcacgaggcg
     2401 ccggtcgcgc accggctgtg cggtgacgaa cgtgccgcgc ccgcgctcgg cggaaacgag
     2461 gccgtcgcgc cccagcaact ccatcgcctg gcgcaccgtg acgcgcgcca cgtcgaactc
     2521 gcgcatcagc gcctcgagcg tcggcagctt ggtgcccggc ggccagtgat tgcgcgcgat
     2581 gcgctggcga aagacgtcgg cgagctgcgc gtaacgcggc atcgggctgt cgcgaaaaag
     2641 ccgcgcctcg tcgctgcctg agcgcttgtg cataaggttc tgtctatagt accttttgcg
     2701 tctttcgcag gcaatcgcct ccggctccct gtcatgcgct cgcctctcga ggcgccgccg
     2761 tgaagggacg ggcggggcac gcaaccgtcg acattccccg tggcacgccg ccccgccctc
     2821 atcgccctgc cgtggctgat cgtcgtcggc atctggtatg cgatcgccta ctcggggctc
     2881 atcaacccgt cgctggtgcc gaccccgcat cgcgtcgcat ggcgtttctg ggagctgctt
     2941 accacggccc ggctgccgct cgacatcctg atgtcgacgg ggcgtgtcgt cgtcggcgtc
     3001 gcactcggca tcacgctcgc ggtgccggtc ggattcgtcc tcggctggta tcccaacgtg
     3061 cgcgcgttca tcgacccgct gatgaacttc ttccgcgcgc tgccgccgat cgcgctgatc
     3121 ccgctcgtga tcgtctactt cggcatcggc gagatcgcga agatcgtcgt gctgttctac
     3181 gcgtcgttct tcgccggcgt gatcgtcatg tacgaaggca ttgcgcagat cagcccgatc
     3241 tacatacggg tgtcgaagac actgggcgcg agcgatgtgg agattttccg caaggtgatc
     3301 gtgccgctca ccgtgccgca cctgctgacc gcgctgcgcg tggcgctcgg cgtcgcctgg
     3361 gcgacgctgg tcgcatccga gctcattgcc gcacagcagg gtctcggcgc gctgatccag
     3421 aatgcgtcgt cgttcttcca gctcgacatc atctacgtcg gcatcatctg catcgggctg
     3481 atcgcactcg caatggacct cgtgctgcgc gcgatcgcgc gccgcctcgt cgcgtggcag
     3541 gagcggatag cgtgagcgcg agggccgaag gcaaggcccg aaggggccgt ccgagcgcga
     3601 gcgcaggagg ccgacgcgcc gacctcacgt cgggcgagat gctgcaagga ggccgagcgt
     3661 gatgagcgcg acgccgcgca tcgtcttcga ccacgtgtcg atggagttcc agtcgaagca
     3721 ggggccgctt cgcgtcgtcg acgacgtgtc gtatgcgatc ggcgacggcg acttcgtggc
     3781 cgtcatcggc ccgtcgggct gcggcaagac gacgatgatg aacatggtcg ccggattcgt
     3841 cgtgccaacg cacggccgcg tgctgctcga cggccggccc gtcagcgggc cggggcccga
     3901 tcgcggcgtc atcttccagg aatacggcgt ctttccgtgg ctgacggtca ggcagaacat
     3961 cgcgttcggc ctgacgctcg cggccaaccg cgcgcacgca cccgaccgcg atgcgatctg
     4021 cgcgcgctac atgaagctca tgggcctctc cgacttcgcc gatgcgtggc cgaagatgct
     4081 ctccggtggc atgcggcagc gcctggcgct cgcacgcgcc tacgcggtaa ggccgcagtt
     4141 cctgctgatg gacgagccgt tcggcgcgct cgacgcgcag acgcgcaacg cgatgcagga
     4201 tttgctgctc gaggtgctgg cgaccgaagg caagacggtg atgctcatca cgcattcggt
     4261 cgaggaggcg atctacctcg cgtcgcgcat cgtcgtgatg agcgcgcggc cgacgcgaat
     4321 ccgcgaggag atcaccgttc cgtttccgta tccgcgctcc gagtcattgc atgaaacgcg
     4381 cgagttcaac gagctgcgct cgcatatacg cgagctcgtg atgaaggagt acgccgctca
     4441 ggcgcaactg gcggggtcgg ccacgacttt cgcagcgccg atgtgatcgc aatcggggtc
     4501 ggagacgact ttcgaatcgc gccgatcgcg cacgcgtaga aagtcgtctc tgaccccact
     4561 tcggtttttt taccaaccgc aaaggaggaa atcatgaaag tgaatcgcag acagttcctg
     4621 cagacaacgg ccgcggtagg catcggcgcg ggtgccgccg tgctcggtgc gccggcgatc
     4681 gcgcaaagcc gcgcgaaggt gaaggtgggc tacctgcata cgctcgccgt cgacggccag
     4741 atctggaccg gcatcgatcg cggctcgttc gccaagcaca acgtcgagct cgacctcgtg
     4801 cagttcacca ccggcctcga gctcttccag gcgatgatcg gcggcagcat cgagatgctg
     4861 gccaccggag cggtcatctc gaactttccc gcccgcgggc aggggaagat gttcctcgtc
     4921 aacgacgtcg aattcgccac cgcgcagctg tgggtgcgcg gcgaccagat caagagcttc
     4981 gccgatctca agggcaagaa gatctcgacg accaccggca ccaccgcgca cgtgtttctc
     5041 gacaccgcgc ttcgcgccaa caacgtcgac ccgaaggacg tcgagattgt caaccagcgg
     5101 atgtccgagg cggtgacgtc gttcatctcg ggcgccgttc ccgcggtcgc gctgtgggtg
     5161 ccgttcaaca tcccggtgcg cgagaaggtg ccgaacgcga agatgctcgc cgacgcgtcg
     5221 gcgtactacc cgaaggcggc catcgtgggc ggctgggcgg tgcgcgacga ttactacgcg
     5281 aagaaccgcg ccgagtgcgc caacatcatc cgcggatggg ccgaggcgaa cgactacatg
     5341 atcgccaacg ccgacgccgc actcgatgcg ctgcagaagc accactacca gcaggtgccg
     5401 ctgtccgacc tcaaggagca gtacaaggca cagaagatgt tcacgtcgaa ggactggaag
     5461 aagctctatg ccgacggcac cgtgacgaac tggctgcagc aggtcaccga cttcttcgtg
     5521 cgcttcggcg gcatccagaa tccggtgcct gcctcgacgt acttcgatcc gaagctctat
     5581 ctcgacacga tcaaggcctg agccgatggc cgaggcttcc gacacgttcg actacgtgat
     5641 cgtcggcgcc ggttccgcgg gatgcatcct cgcgaaccgg ctgtcggccg acgcgacgac
     5701 gcgcgtgctg ctgctggagg ccggcggttc cgatcgcaat ttctggatcc gcctgccggt
     5761 cggctatttc cgcacgatct acgatcctcg cttcgcgcgc ctgttcgaca cggtgccgtc
     5821 ggaaggcacc gcgggacgca acgtgctgtg gccgcgcggg cgcgtcatcg gcggctcgtc
     5881 gtcgatcaac ggcctcgtct acatccgcgg gcagcacgag gacttcgacg actgggcggc
     5941 gcagggcgcg gcggggtggc gctacctcga cgtgctgccc gatttcaagc gcatcgagca
     6001 ctatgcgggc ggggcgagcg cgtatcacgg cgcgtcgggc gagctcggcg tatgcaatct
     6061 gcgcaacgac catccatact gcagcgcatg ggtcgatgcc ggcgttgaat ccgggctgcc
     6121 gcgcaatccc gatttcaacg gcgccacgac gtatggcgtc ggctcgtatc aattgagcat
     6181 cgacaacggc tggcggcaaa gcgcagcggt cgcgtttctg cggcccgcaa gccggcgccc
     6241 gaacctctcg atcctgccgc gcgcgcttgc cacgcgcgtg cgtttcgaag gaacgcgcgc
     6301 cctcggcgtc gagtggcagc gtggcgggca gacgcatttc gcgcgtgccg cacgcgaagt
     6361 cattctttcc gcggggacca tccagtcgcc gcagcttctg cagctctcgg gcgtggggcc
     6421 gcccgcactt ctgcgtgggc tcggcatccc ggtcgtggcc gggctttccg gcgtcggcga
     6481 gaatctgcag gatcactatc aggcacgcac gatcgtgcgg cttcgcaagc gcgtctcgct
     6541 caacgacgac gtgcgcaatc cgctgaagct cgcgggcatg ggggccgaat ggctgtttcg
     6601 ccgctcgggg ccgctcacgg tgggtgcggg gcaggtcggc ggcgccgcgt gcaccgagca
     6661 tgcgaccggc aaccggcccg acgtgcagtt caacgtgatg ccgctgtcgg tcgacaagcc
     6721 cggggatccg ctgcatccgt attcgggctt caccgcggcc gtgtggcagt gtcatcccga
     6781 gtcgcgcggg cgactcgcga ttcgctcgac caatcccgcg gatgcgccgg gcatcgagcc
     6841 ccgctacctc gacgccgatc gcgaccgcaa gacgctcgtc gccggcatca ggatgctgcg
     6901 cgacatctat cggcaaccgt cgttccgcga gctgttcgac gccgaaatgc ttcccggcac
     6961 cgacgcgaga tacgacgagg acctgctcga cttcgcacgc cacgcgggcg gcaccgtctt
     7021 ccattgtgtc ggcacctgcc ggatggggga tgcgcgcgac gacgacgccg tcgtcgatcc
     7081 gcagcttcgt gtgcgcggtg tcgaggcgtt gcgcgtgatc gatgcatccg tgatgccgag
     7141 cgtaacgtcg gcgaacacga atgcggcgtc gctgatgatc ggcgagcacg gtgcgcgcat
     7201 cgtgctgcag ggcggctgag ctcgcgtgac ggcgaagccg gtaccgaagc agggtcagag
     7261 acgacttttc cgctgaccct cggaaagtcg tctctgaccc tctgtcacgc cacgtacacg
     7321 gtcttgatgt tgacgaactc cctgatgcca tagctcgcga gctcgcggcc atggccgctc
     7381 tccttgatgc cgccgaacgg cagccgcgga tcggagcgca ccgcatcgtt gacgaacacg
     7441 cagccgcttt cgatgaggtc tgcggcaatg cgctcgccgc gcgcgatgtc gcgggtgatc
     7501 acgccggcgc cgaggccgaa agccgacgcg ttggccatcg ccaccgcctc ggcttcgtca
     7561 cgaacgggca cgacggcggc aaccggcccg aacaattcct cgtcgaacgc cggcataccc
     7621 ggccgcacgt cggtcagcac cgacggcgga taccaggctc ccggaccctc gggaatcgcg
     7681 ccgccgagca tgcatcgcgc gcctttcgcg atgcttcgat ccacctggcg ctgcagcgtg
     7741 tcgcgcagat cggcccgtgc ctgcggaccg atctcggtcg attcgtcgag cgggtcgccc
     7801 atcttcgacg ccgccatccg cttgacgaag cgcctctcga actccgcgcg cacgcgctcg
     7861 acgacgatga agcgcttgcc ggcgatgcag ctctgtccgg agttgacgag ccgcgccttc
     7921 gtgcacacgt cggcggcgag tgccagatcg gcgtcctcga gcaccagata cggatcgctg
     7981 ccaccgagct cgagcaccgt cttcttgagc agctgcccgg ccttcgcggc aatcgcccgg
     8041 ccggcgggac cgctgccggt cagcgtgacg gcgcgcacca gcggatcctc gatgaccgcg
     8101 tcgacgctct tgctgccgat catcagcgtg cggaacaggt tctccgggaa tcctgcgccg
     8161 cggaacacgt cctcgatcgc gagcgcgcat cccggcacgt tcgacgcgtg cttcagcacg
     8221 gcggcattgc ccgccatcag gccgggcgcg gcgaagcgga agacctgcca gaacggaaag
     8281 ttccacggca tcacggccag cacgacgcca agcggcttga acgtgacgaa gctcgagcgc
     8341 gcctcggtcg gcacggtctc gcgcgccagg aacgcggcgg cgtgctcggc gaaatagtcg
     8401 caagccgttg cgcatttctg cgcttcggcg actccgtcgc gtaccggctt gcccatttcg
     8461 cgtgccatca gatgcgcgaa atcgcgcgcg ttcttgcgca ggattgccgc ggcccggcgc
     8521 atcggcacgg cacgctcggc atacgaagcg tggcgccagt cgacgaacgc gccgtgtgcc
     8581 gatttgacga tgccggcgac aagttgcggc gtcatctcgt cgtgggtggc gatgacttcg
     8641 tcggttgcgg gattgatggc ctggatcgac atggaggttc tcgctcgcgg gattcaacac
     8701 tttggacaac ggcggcggaa cgaaaagtcc gctcacggca caatagcggc atgccgcgca
     8761 atcgctcgat gggaccgcgc ccatgagtgc cgaagggccg ccccgaggcg cgaattccgc
     8821 tcccgcgggg ggcagcgcag cggcgaagcc gcaagcgtgg ggggatcatc cgaaggcgag
     8881 cgccgccgag cgcgtgactc atcccgagcg gcggtcgctg cgcgagcgga tgagcggcat
     8941 gcgcaatatc ccgccgttcc tggcactcgt ctggcagacg agcccgtcga tgatgctcgg
     9001 cgaggccgtc ctgcgtgtgg tccgcgcgct gctgcctgtc gtcacgctct acgtcggcaa
     9061 gctcatcatc gacgaggtcg tcctgctgac gaaaacgccg ccgccgggcg acgagcttcg
     9121 cgcgtggctc gaaagcggcg cgctcgatcg catcgtgtcg cttctcgcgc tggaattcgg
     9181 attggccgtg ctgtccgacg tgctcggacg cgcggcgtcg ctgctcggcc agctcttcgg
     9241 ccaggcgcag gacatcgtca cgatcgtgag ctt
//
LOCUS       DASSVX010000219        11304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_342495, whole genome shotgun sequence.
ACCESSION   DASSVX010000219 DASSVX010000000
VERSION     DASSVX010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342495"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFO53_09410"
     CDS             complement(<1..464)
                     /locus_tag="VFO53_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET8876532.1"
                     /translation="MTSPLLANLNPEQLEAVTLPHASSLILAGAGSGKTRVLTTRIAW
                     LLATGQASPLSILGVTFTNKAAKEMLARLAAQTPVNIRGMWVGTFHGLCNRMLRMHHR
                     EAGLPQLFQILDTQDQLALIKRMYRAHDIDGEHYPPRELQWFIASSKEEGLRP"
     gene            523..1872
                     /gene="argA"
                     /locus_tag="VFO53_09415"
     CDS             523..1872
                     /gene="argA"
                     /locus_tag="VFO53_09415"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HET8876533.1"
                     /translation="MARALDETPPVNSATMRAFVHWIRAAAPYVHAFRGKTFVIAFGG
                     EVVADESFLGVVHDLNLLHSLGIRLIVAHGCRPQVEAILAAQSIESRYANGVRITDND
                     AMDCVLEAAGQVRARIEALLSLGLANSPMAGARNRVSSGNYLTAKPMGVVDGVDMQLS
                     GEVRRIDTEAIQQRLDDGDIVLISPTGYSPTGEIFNLTVEEVATQCAVRMDATKLIFL
                     TDADGVRNARRQLLSDLSTSDAEKLLARPERLPPDIRVYLPAAIRACNNGVARAHLIS
                     RHHDGALLLELFTRDGVGTMIAATALAHIRNATIDDVGGILSIIEPLEEQGVLVRRSR
                     ERLESEIERFVVAEYDNHIIGCAALYAFPAERLGELAALAVHPDFRREGYGEALMSEI
                     EQRARKLKLAQLFVLTTKTAHWFVERGFRIGSVADLPQQKQALYNYQRKSQVYVKNL"
     gene            1912..2184
                     /locus_tag="VFO53_09420"
     CDS             1912..2184
                     /locus_tag="VFO53_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HET8876534.1"
                     /translation="MARMVKCVKLGREAEGLDFPPYPGELGKRIYENVSKEAWAQWLK
                     HQTMLVNENRLSLADARARKWLAEQMEKHFFGTGAEMPAGYVPPSQ"
     gene            2181..2807
                     /locus_tag="VFO53_09425"
     CDS             2181..2807
                     /locus_tag="VFO53_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275763.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HET8876535.1"
                     /translation="MKTEHAATAPPEAPPISRYPVPALDALPDDIRARILDVQEKAGF
                     VPNVFVTLAHRPDEFRAFFAYHDALMLKEGGLTKAEREMIVVATSGANDCLYCIVAHG
                     AILRIYAKNPLVADQVAVNYRKADITARQKAMLAFAMKVATNSVAIDDADFARLHEHG
                     LCDEDIWDIGAIAAFFALSNRMANLIAMRPNDEFYAMGRLPRQRSEPA"
     gene            2824..3591
                     /locus_tag="VFO53_09430"
     CDS             2824..3591
                     /locus_tag="VFO53_09430"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET8876536.1"
                     /translation="MGQALLAGNATLIATLAAVLVIGALIGSIGIGGILLSPWLIEIV
                     GLDVRDAITISTASFVATGLVALVVFARGERGALARGWPLIVATVPGALLGAIALAVA
                     PRRLTLALLAALLIATGLRMLASGRSRTRRNGEPSTGVDWAVGVLSGFLSALTGTGGP
                     MVLVPIQLWRGAPVLAAIGVGQVVQLPIAATATAGNWMSGGVDLPAAALVGAMLLPGV
                     IVGPRLAAAVPIAALTRIVAVLLLCVGGWFVVRAATG"
     gene            complement(3629..4903)
                     /locus_tag="VFO53_09435"
     CDS             complement(3629..4903)
                     /locus_tag="VFO53_09435"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET8876537.1"
                     /translation="MKLEIRNGRLIDPARALDRVASLFIDGHMIAGSGEAPRGFRADR
                     VIEAGGCVVSPGFVDLAARLREPGLEYKATLESELAAAAAGGVTSLACPPDTDPPLDE
                     PGLVQMLKHRARLIGLAHVYPVGALTVGLDGTTLTEMGQLAEAGCVAFSQADAPFHDT
                     LMLLRALQYAGTFGHRVWLRPQDHYLAQGGVAHDGEVATRLGLPAIPVAAETIALSKI
                     FGLVRATHVGVHLTRLSSAEGVAMVRAAKAEGLPVTCDVDVHHLHLCDVDIGWFDPQA
                     HLVPPLRSTRDREALQRGVADGTIDAICSDHAPVDDDGKQMPFGEAEPGASALELLLP
                     LTLKWAAVAGVALGDALGCITTRPAAILGLARPSLADGMAADLCVFDPASHWTVQRDA
                     LRSQGKNTPFLGLEVVGRVRATLLGGRVVHEA"
     gene            complement(5048..6010)
                     /locus_tag="VFO53_09440"
     CDS             complement(5048..6010)
                     /locus_tag="VFO53_09440"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET8876538.1"
                     /translation="MTRNPQLNRNGELTHLLTLEGLPAAVITHILDTAEPFVSIAERE
                     VKKVPLLRGKAMFNLFFENSTRTRTTFEIAAKRLSADVINLNIGVSSTAKGETLLDTV
                     DNLCAMNADLFVVRHAQSGAPHLIASHLTATGRSHVHVVNAGDGRHAHPTQGLLDLYT
                     IRHYKKDFRELSVAIVGDVLHSRVARSLIHGLTTMQVPRVRVIAPRTLLPTAVEALGV
                     EVMNDMREGLRGCDVVVMLRLQNERMKGALLPSAGEYFKHYGLTSEKLALAKPDAIVM
                     HPGPMNRGVEIDSPVADGSRSVILPQVTFGIAVRMAVLSIVASS"
     gene            complement(6024..6566)
                     /gene="pyrR"
                     /locus_tag="VFO53_09445"
     CDS             complement(6024..6566)
                     /gene="pyrR"
                     /locus_tag="VFO53_09445"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET8876539.1"
                     /translation="MTPDDALPDAEAALAALVLRMQGAVAHDAALVGIYSGGAWLAER
                     LAAVLPGAHPLGFIDVSYYRDDYAVSGLKPNVKRTSLPFDVSGAAIVLVDDVLYTGRS
                     VRAAINELFDFGRPERIELAVLVDRGGRELPIEATYTGARLAVARDLSIVLSRDCEGT
                     LSLGVEPARRRERAEPRVAR"
     gene            complement(6556..6990)
                     /gene="ruvX"
                     /locus_tag="VFO53_09450"
     CDS             complement(6556..6990)
                     /gene="ruvX"
                     /locus_tag="VFO53_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET8876540.1"
                     /translation="MRREADATVLAFDFGTRRIGVAVGNTLMRVAHPLATIAAADPAA
                     RWTAIRALLDEWRPQRLVVGLPVHADATPHAMTARAREFALELADRFALPVALEDERH
                     TSEIARDALAAAGRGGRAHRPLRDRVAAQIILQAWFDGRHDA"
     gene            complement(6998..7561)
                     /locus_tag="VFO53_09455"
     CDS             complement(6998..7561)
                     /locus_tag="VFO53_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET8876541.1"
                     /translation="MGESVNLTHHFLIAMPAMADPHFANSLTYVCEHNPEGALGIVVN
                     KPIDLTLSALFEQIEIPLADRALCEAPVHFGGPVQLDRGFVLHRPIGNWQSTLPIHDE
                     VGLTTSKDVLEAAGRGEGPKDMFVSLGYAGWQAGQLEQELAQNAWLTVEADADVLFDL
                     PAERRLPAAVRLLGIDISRLSDTAGHG"
     gene            complement(7627..8349)
                     /locus_tag="VFO53_09460"
     CDS             complement(7627..8349)
                     /locus_tag="VFO53_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET8876542.1"
                     /translation="MRYLILAAGMGRRLGAAGAGLPKCLIEVNGETLIARLLRQIRRN
                     DPAADIHVVLGYRSDAVTPAVHGCRIVLNPFFDTTGINASMWFARDAFDRPLMLIHAD
                     LVLADDLADALLTAEAETLMAFDSTLRDPTEVNVAVAAGRVTRFDENFTGYSGLYAGV
                     LKLSEPAASAFAQTLDQRVRQGFDTPRDYYFFAVRAIIEQHAIAVSAFDIAGRAWQEI
                     DRAEHIAAARARFREPRLAIAS"
     gene            complement(8402..9736)
                     /locus_tag="VFO53_09465"
     CDS             complement(8402..9736)
                     /locus_tag="VFO53_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET8876543.1"
                     /translation="MKNLVILHLESISRQRLAAFASAFPNTLRLMQEAIVFDNYFSSA
                     TSTVMVLTYLFHANDFELDTSTQFDGAQPARNNRNVFEILQQRGYHADLICLNGFPAR
                     PIRLSAWGNGLPPVWETNDFPQLFARFDALTDAPPFAIYVWDLISHVEHSMALAPHAN
                     GLTDQLRRACAVADHAVGVFRDTLERKGLLDDTTIVLYGDHGDDFWTHGYKGGMIHGT
                     EPYTDIIAAPLAIRDPDHAPALRADLASTIDIAPTCLSLLGVDAETTFAYSGVDLLQR
                     APEAVYSQNFTANQPDSVRLDIAKAFSVTDDTYTLMASSRGLELFAHRLDPGNHCNLL
                     HLFAFDAAGRLALRALPGTAGHFLASWSRNPQAVARLAHDFERLRERLQTRIEAKRRY
                     IDERGVEAAHALDRRCMTTISARGRDAFFNARVSAPAAGAAAAAFEYAWKLQ"
     gene            complement(9748..>11304)
                     /gene="fusA"
                     /locus_tag="VFO53_09470"
     CDS             complement(9748..>11304)
                     /gene="fusA"
                     /locus_tag="VFO53_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET8876544.1"
                     /translation="DGGRRVVDCFFTPSGEADFSSVAAAHSALVDQVVEVDEALMSLY
                     LEQGEIAPEQLHAPFEKALREGHLVPVCFVSARTGAGVGELLTVFDKLLPNPKEGNPP
                     RFTKGEGESAVEFHSEPDPSKHVLAHVFKVVMDPFVGKLGVFRVHQGTVTRDTQLFVG
                     DGRKPFKVGHLFMLQGAKYIELDRAVPGDIAAIAKVEEIVFDCVLHDSHDEDHLHMRP
                     LEFPVPMHGFAITPKRRGDEQRISDVLHKMIAEDPTLVVAHDAIVNETVLRGLGELHL
                     RSVFERMATQFKLEVETRPPRVPYRETVTSKAEGHHRHKKQTGGAGQFGEVYLRIEPM
                     PRGAGFEFVDQVKGGAIPNTLIPAVQKGVEQVLTSGPVAGFPMQDVRVIVYDGKYHPV
                     DSKEVAFVAAGRKAFLDAITKARPIVLEPIVGMEINCPANTMGDVAGDLSSRRGQVTG
                     TQTLQANMLAVNGYAPLAELDGYAARLKSMTGGHGAWTMSLSHYEQAPPNLQNQLSTE
                     YAKHRRHEEE"
BASE COUNT         1801 a         3817 c         3922 g         1764 t
ORIGIN      
        1 ggccgcaggc cctcctcctt cgacgacgcg atgaaccact ggagctcgcg cggcggatag
       61 tgctcgccgt cgatgtcgtg cgcgcgatac atccgcttga tcagcgcgag ctggtcctgc
      121 gtgtcgagga tctggaaaag ctgcggaagg cccgcctcgc ggtggtgcat ccgcagcatc
      181 cggttgcaca ggccgtggaa ggtgccgacc cacatgccgc ggatgttgac gggcgtctgc
      241 gccgcgagcc gcgcgagcat ctccttcgca gccttgttcg tgaacgtgac gccgagaatc
      301 gacagcggac tcgcctggcc ggtggcgagc agccacgcaa tgcgcgtggt gagcacgcgg
      361 gtcttgccgc tgcccgcgcc ggcgaggatc agcgaggacg cgtgcggcag cgtgaccgct
      421 tcgagttgtt ccggattgag attggcgagg agcggcgagg tcactgactg tcgattatag
      481 gtgaacgcta taatcgcgcc cgctcatgga gaacagtcgg taatggcacg cgcgctcgac
      541 gagacgccgc cggtcaactc ggccacgatg cgcgcgttcg tgcactggat acgcgcggcc
      601 gcgccgtacg tgcacgcgtt tcgcggcaag acgttcgtca tcgcgttcgg cggcgaggtc
      661 gtcgccgacg agtcgttcct cggcgtcgtc cacgacctca acctgctgca cagcctcggc
      721 atcaggctga tcgtcgcgca cggctgccgc ccgcaggtcg aggcgatcct ggccgcgcag
      781 agcatcgaga gccgttacgc gaacggcgtg cgcatcaccg acaacgacgc gatggattgc
      841 gtgctcgagg cggcaggcca ggtgcgcgcg cgcatcgagg cgctcctgtc gctcgggctc
      901 gcgaactcgc cgatggccgg tgcgcgcaac cgcgtctcga gcggcaacta cctgacggcg
      961 aagccgatgg gcgtcgtcga cggcgtcgac atgcagctgt cgggcgaagt gcggcgcatc
     1021 gacaccgagg cgatccagca gcggctcgac gacggcgaca tcgtgctgat ctcgccgacc
     1081 ggctattcgc cgaccggcga gattttcaac ctgacggtcg aggaggtggc gacgcaatgc
     1141 gcggtgcgca tggatgcgac gaagctgatc ttcctcaccg atgccgacgg cgtgcgcaat
     1201 gcgcggcgcc agcttctgtc ggacctgtcg accagcgacg cggaaaagct tctcgcgcgc
     1261 cccgagcgcc tgccgcccga catccgcgtc tacctgccgg cggcgatccg cgcgtgcaac
     1321 aacggcgtgg cgcgcgcgca cctgatcagc cgccaccacg acggcgcgct tctgctcgag
     1381 ctctttacgc gcgacggcgt cggcacgatg atcgccgcca ccgcgctggc gcacatccgc
     1441 aacgcgacga tcgacgacgt cggcggcatc ctgtcgatca tcgaaccgct cgaggagcag
     1501 ggcgtgctgg tgcgccgctc gcgcgagcgg ctcgaaagcg agatcgagcg cttcgtcgtc
     1561 gccgagtacg acaatcacat catcggctgc gcggcgctgt acgccttccc ggccgagcgc
     1621 ctcggcgagc ttgcagcact cgccgtgcat ccggattttc gccgcgaagg ttacggcgag
     1681 gcgctgatga gcgagatcga gcagcgcgcg cgcaagctca agctcgcgca gctgttcgtg
     1741 ctgacgacga agaccgcaca ctggttcgtc gagcgcgggt ttcgcatcgg ctcggttgcc
     1801 gacctgccgc agcagaagca ggcgctctac aattaccagc gcaagtcgca ggtttacgtc
     1861 aagaatctgt aacgggcgag cgcccgttgc gcgtcaggca aaggagcggc gatggcacga
     1921 atggtgaaat gcgtgaagct cggacgcgag gccgagggcc tcgattttcc gccgtatccc
     1981 ggcgagctcg gcaagcgcat ctacgagaac gtgtcgaagg aagcgtgggc gcagtggctc
     2041 aagcaccaga cgatgctcgt caacgagaac cggctgtcgc tcgccgacgc gcgcgcccgt
     2101 aagtggctgg cggaacagat ggagaagcac ttcttcggca cgggcgcaga gatgccggcg
     2161 ggttacgtgc cgccgtcgca gtgaagacgg aacacgctgc cacggccccg cccgaggcgc
     2221 cgccgatctc gcgctacccg gtgccagcac tcgacgcgct tccggacgac atccgagcca
     2281 ggatcctcga cgtgcaggaa aaggcgggct tcgttccgaa cgtattcgtc acgctcgcgc
     2341 accgtcccga cgaattccgc gcgttcttcg cataccacga cgcgctgatg ctgaaggaag
     2401 gcggcctgac gaaggccgag cgcgagatga tcgtcgtggc gacatcgggt gccaacgact
     2461 gcctctactg catcgtcgcg cacggcgcca tcctgcgcat ctacgcgaag aacccgctcg
     2521 tcgccgatca ggtcgctgtc aactatcgca aggccgacat caccgcgcgc cagaaggcga
     2581 tgctcgcgtt cgcgatgaag gtcgccacga acagcgttgc gatcgacgac gccgatttcg
     2641 cccggttgca cgaacacggc ctttgcgacg aggacatctg ggacatcggc gccatcgccg
     2701 cttttttcgc gctgtcgaac cggatggcga acctgatcgc gatgcggccg aacgacgagt
     2761 tctatgcgat ggggcgcctg ccccggcaac gaagcgaacc cgcctgaacg tgcggcggcg
     2821 ggtttgggcc aggcgctcct cgcaggcaac gctacgctga tcgcgacgct ggccgcggtg
     2881 ctcgtgatcg gcgccctcat cggcagcatc ggcatcggcg gcattttgct gtcgccctgg
     2941 ctgatcgaaa tcgtcgggct cgacgtgcgc gacgcgatca ccatcagcac ggcgagtttc
     3001 gttgcgaccg gcctcgtcgc gctcgtcgtg tttgcgcgcg gcgagcgcgg ggcactggcg
     3061 cgcggatggc cgctgatcgt cgcgaccgtg ccgggcgcgc tgctcggcgc cattgcgctc
     3121 gccgttgcgc cgcgacgcct gacgttggcg ctgctcgccg cactgctgat cgccaccggc
     3181 ctgcgcatgc tggcctccgg tcggtcgcgc acgcgccgca acggcgagcc gtcgactggc
     3241 gtcgattggg cggtcggcgt gctgagcggg ttcctgtcgg cgctcaccgg caccggagga
     3301 ccgatggtgc tggttccgat ccagctctgg cgcggcgcac cggtgctggc cgcgatcggc
     3361 gtcgggcagg tggtgcagct tccgatcgcc gccacggcaa ccgcgggcaa ctggatgagc
     3421 ggcggcgtcg atctcccggc cgccgcgctg gtcggcgcca tgctcttgcc cggcgtgatc
     3481 gttggaccac ggctcgcagc agcggtgccg atcgccgcgc tcacgcggat cgtcgccgtg
     3541 ctgctgctgt gcgtcggtgg ctggttcgtc gtgcgcgcgg cgaccggctg acgcggccgc
     3601 tacgctgccc ggcgcggcgc cgtgtgcttc acgcctcgtg cacgacgcgt ccgccgagga
     3661 gcgtcgcccg cacccggccg acgacttcga gaccgaggaa cggcgtgttc ttgccctggc
     3721 tgcgcagcgc gtcgcgctgc accgtccagt gcgacgccgg gtcgaagacg caaaggtcgg
     3781 ccgccatgcc gtcggcgagc gaggggcggg cgagcccgag gattgccgcg ggccgcgtcg
     3841 tgatgcaacc gagcgcatcg ccgagcgcga cgccggccac cgcggcccac ttgagcgtga
     3901 gcggcagcag cagctcgagc gcgcttgccc cgggctcggc ctcgccgaac ggcatctgct
     3961 tgccgtcgtc gtcgaccggc gcatgatccg agcagatggc gtcgatcgtg ccgtcggcaa
     4021 cgccgcgctg cagcgcttcg cgatcgcgcg tgctgcgcag cggcggcacg aggtgcgcct
     4081 gcgggtcgaa ccagccgatg tcgacgtcgc aaagatgcag gtgatgcacg tcgacgtcgc
     4141 aggtgaccgg cagcccctcc gccttggcgg cgcgaaccat cgccacacct tccgcggacg
     4201 aaagccgggt caggtgcacg ccgacgtgcg ttgcgcgcac gagcccgaaa atcttcgaca
     4261 acgcgatcgt ttcggcggcc accggaatcg cgggaaggcc gagccgcgtg gccacctcgc
     4321 catcgtgcgc gacgcccccc tgcgcgagat agtggtcctg cgggcgcagc cacacgcgat
     4381 ggccgaaggt ccccgcgtat tgcagcgcgc gcagcagcat cagcgtgtcg tggaatggtg
     4441 cgtcggcctg cgagaacgcg acgcagcccg cctcggcgag ctgccccatt tcggtgagcg
     4501 tggtgccgtc gagcccgacg gtgagcgcgc cgaccggata cacgtgcgcc aaaccgatga
     4561 ggcgcgcgcg atgcttgagc atctggacga ggccgggctc gtcgagcggc ggatcggtgt
     4621 cgggcggaca cgcgaggctc gtcacgccgc cggccgccgc ggccgcaagc tccgattcga
     4681 gcgtcgcctt gtactcgagg cccggctcgc gcaagcgcgc cgcgaggtcg acgaagcccg
     4741 gcgagacgac gcagccgccc gcctcgatga cgcgatccgc gcgaaagccg cgcggcgctt
     4801 cgccgctgcc cgcgatcatg tggccgtcga tgaacagcga tgcgacccgg tcgagcgcgc
     4861 gcgcgggatc gatcagccgg ccgttgcgga tctcgagctt catgtcagtc gggaagggcc
     4921 gccccgaccg actggcgcgc cttcttgggg ggcagcgagc gcgtgagccc gacgggccgc
     4981 cccgagggcg aacacccgag tgcgaagcac gaggatcgtc gaaccgagcg tggggggatg
     5041 ttccatctca gctggaggcg acgatcgaca gcacggccat gcgcaccgcg atgccgaacg
     5101 tgacctgcgg caggatcacc gaacgcgagc catcggcgac cggcgagtcg atctcgacac
     5161 cgcggttcat cggccccgga tgcatcacga tcgcatcggg cttggcgagt gccagctttt
     5221 ccgacgtgag cccgtagtgc ttgaagtact cgccggccga cggcagcagc gcgcccttca
     5281 tccgctcgtt ctgcagccgg agcatgacga cgacgtcgca tccgcgcaga ccctcgcgca
     5341 tgtcgttcat cacctcgacg ccgagcgctt cgacggccgt gggcagcagc gtgcgcggcg
     5401 cgatcacgcg cacgcgcggc acctgcatcg tcgtcaggcc gtggatcagc gagcgggcga
     5461 cgcgcgaatg cagcacgtcg ccgacgatcg cgacactgag ctcgcgaaaa tccttcttgt
     5521 aatggcggat cgtgtagagg tcgagcagcc cctgcgtcgg gtgcgcgtga cggccgtcgc
     5581 cggcgttgac gacgtgaacg tgcgagcggc cggttgccgt caggtgcgat gcgatgagat
     5641 gcggcgcccc cgactgcgca tgccgcacga cgaacaggtc cgcattcatc gcgcacaggt
     5701 tgtcgacggt gtcgagcagc gtctcgccct tcgccgtcga cgacacgccg atgttgaggt
     5761 tgatcacgtc ggcagacagg cgcttcgcgg cgatctcgaa cgtcgtgcgc gtgcgcgtcg
     5821 agttctcgaa gaacaggttg aacatcgcct tgccgcgaag aagcggcacc ttcttcacct
     5881 cgcgctcggc gatggacacg aaaggctcgg ccgtgtcgag gatgtgcgtg atcaccgcgg
     5941 ccggcaatcc ctccagcgtc aacagatgcg tgagctcgcc gttgcggttg agctgcggat
     6001 tgcgcgtcat cgaggccccg cgatcatcgc gcgacccgcg gctcggcgcg ctcgcgacgg
     6061 cgcgccggct cgacgccgag cgacagcgtg ccttcgcaat cgcgcgacag gacgatcgaa
     6121 agatcgcgcg ccacggcaag gcgtgcgccc gtgtacgtcg cctcgatcgg caattcgcgt
     6181 ccgccgcgat cgacgagcac cgcgagctcg atgcgctccg ggcgaccgaa gtcgaacagc
     6241 tcgttgatcg ccgcgcgcac gctgcgcccg gtgtacagca cgtcgtcgac gagcacgatc
     6301 gccgcacccg acacgtcgaa cggcagcgac gtgcgcttca cgttcggctt caaaccggac
     6361 accgcgtagt cgtcgcggta gtacgacacg tcgatgaagc ccagcgggtg tgcgccgggc
     6421 agaacggctg cgagccgctc ggcgagccat gcgccgcccg agtagatgcc gacgagcgcg
     6481 gcgtcgtggg caaccgcgcc ttgcatgcgc aggacgagtg ccgcgagcgc cgcctcggcg
     6541 tcggggagcg cgtcgtcagg cgtcatggcg cccgtcgaac caggcctgca ggatgatttg
     6601 cgcagcgacg cgatcgcgca gcggccggtg cgcgcggccg ccgcggcccg ccgcggccag
     6661 cgcatcgcgc gcgatctcgc tcgtatggcg ctcgtcctcg agggccaccg gaagcgcgaa
     6721 gcgatcggca agttcgagcg cgaattcgcg tgcgcgcgcc gtcatcgcat gcggggttgc
     6781 atcggcgtgc acgggcaacc cgacgacgag ccgctgcgga cgccattcgt cgagcagcgc
     6841 gcggatggcg gtccagcgtg cggcggggtc ggccgcggcg atcgtcgcaa gcggatgggc
     6901 gacgcgcatc agcgtgttgc ccacggcgac gccgatccgg cgcgtgccga aatcgaacgc
     6961 gagcaccgtc gcgtccgctt ccctgcgcat tgacggctca cccgtgaccg gcggtgtcgg
     7021 aaagccgcga gatgtcgatg ccgagcaggc gcaccgcggc aggcagccgg cgctcggccg
     7081 gcaggtcgaa cagcacgtcg gcatcggcct cgaccgtgag ccacgcgttc tgcgcgagct
     7141 cctgctcgag ctgccccgcc tgccatccgg cgtagccgag cgacacgaac atgtccttcg
     7201 gtccttctcc ccggccggcg gcctcgagca cgtccttgga cgtcgtgagg ccgacctcgt
     7261 cgtggattgg cagcgtcgac tgccagttgc cgatcggccg atgcaggacg aacccgcgat
     7321 cgagctgcac cggaccgccg aaatgaacgg gcgcttcgca cagcgcgcga tcggcgagcg
     7381 gaatctcgat ctgctcgaac aacgcggaaa gggtgaggtc gatcggcttg ttgacgacga
     7441 tgccgagggc gccttcggga ttgtgctcgc agacgtacgt gagcgagttc gcgaagtgcg
     7501 gatccgccat ggccggcatg gcgatcagga agtggtgtgt gagattcacc gattcgccca
     7561 cggtcgcgat tgtaatgtgc cgcggccggg cgcgccactg cgcatggccg gcgcccgagc
     7621 gctcgctcac gatgcgatcg cgagccgcgg ctcgcggaag cgggcgcgcg ccgccgcgat
     7681 gtgctccgcg cgatcgatct cctgccaggc gcggcccgcg atgtcgaagg ccgacaccgc
     7741 gatcgcgtgt tgctcgatga tcgcccgcac cgcgaagaaa tagtagtcgc gcggcgtgtc
     7801 gaagccctgg cgtacacgct ggtcgagggt ttgcgcgaac gcgctggcgg ccggctccga
     7861 aagtttcagc acgccggcgt acaaaccgct gtagccggtg aagttctcgt cgaagcgcgt
     7921 gacccggccc gcggcgaccg cgacgttgac ctcggtcggg tcgcgcagcg tgctgtcgaa
     7981 ggccatcagc gtttccgctt ccgccgtcag cagcgcatcg gcgaggtcgt cggccagcac
     8041 cagatcggcg tgaatcagca tgagcggccg gtcgaacgcg tcgcgcgcga accacatcga
     8101 cgcattgatg cccgtcgtgt cgaaaaacgg attgaggacg atccggcatc catgcaccgc
     8161 cggcgtgacc gcgtcgctgc gatacccgag caccacgtgg atatcggccg ccgggtcgtt
     8221 gcgccgaatc tggcgcaaca gccgggcgat cagcgtctcg ccgttcacct cgatgaggca
     8281 cttcggcagt cccgcaccgg ccgcgccgag acgccggccc atacccgcgg cgagaatcag
     8341 gtagcgcatc gacgcgacgg tgctgccggg ctggaggcag cggatgcgcc cgcggccttc
     8401 ttcactgcag cttccacgcg tattcgaatg ccgccgctgc cgcgcctgcc gccggcgcgc
     8461 tcacgcgcgc attgaagaac gcgtcgcggc cacgcgcact gatcgtcgtc atgcagcggc
     8521 gatcgagcgc atgcgcggcc tcgacgccgc gctcgtcgat gtaccggcgc ttcgcttcga
     8581 tccgcgtctg gagccgctcg cgcagacgct cgaaatcgtg cgcgaggcgc gcgacagcct
     8641 gcgggttgcg gctccacgag gcgaggaagt gcccggcggt cccgggcaat gcccggagcg
     8701 caaggcgccc ggcagcgtcg aacgcgaaca ggtgcagcag gttgcagtga tttcccgggt
     8761 cgagccgatg cgcgaaaagc tcgagcccgc gcgagctcgc catcaacgta taggtgtcgt
     8821 cggtgaccga gaatgccttg gcgatgtcaa gccggacgct gtcgggctgg tttgcggtga
     8881 aattctgcga gtacacggcc tcgggtgcgc gctgcagcag atcgacgccc gaatacgcga
     8941 acgtcgtctc ggcgtcgacc ccgagcagcg acaggcacgt gggcgcgatg tcgatcgtgc
     9001 tcgcgaggtc ggcccgcagc gccggcgcat ggtcggggtc gcggatggcg agtggtgcgg
     9061 cgatgatgtc ggtgtagggc tcggtgccat ggatcatgcc gcccttgtaa ccgtgtgtcc
     9121 agaaatcgtc gccgtggtcg ccgtagagga cgatcgtcgt gtcgtcgagc aggcccttgc
     9181 gctcgagcgt gtcgcggaag acgcccaccg cgtggtcggc gaccgcgcag gcgcggcgca
     9241 actggtcggt cagcccgttg gcgtgcggcg cgagcgccat gctgtgctcg acatgcgaaa
     9301 tgaggtccca gacgtagatt gcgaacggcg gcgcatcggt caatgcgtcg aagcgcgcga
     9361 acagctgtgg aaaatcgttg gtctcccaga ccggcggaag cccgttcccc catgccgaca
     9421 ggcggatcgg ccgcgccgga aagccgttga ggcagatcag gtccgcgtgg tagccgcgct
     9481 gctgcaggat ctcgaatacg ttgcgattgt tgcgcgccgg ctgcgcgccg tcgaattgcg
     9541 tcgacgtgtc gagctcgaaa tcgttcgcat ggaaaaggta ggtcagcacc atcaccgtcg
     9601 acgtcgccga cgagaagtag ttgtcgaaga cgatggcttc ctgcatgagg cgcagcgtat
     9661 tgggaaacgc cgatgcaaac gccgcgaggc gctgccgcga gatgctttcg aggtgcagga
     9721 tgacgaggtt cttcaacgga ttgcccgcta ctcttcctcg tgccggcggt gtttcgcgta
     9781 ctcggtcgac agctggttct gcaggttcgg cggcgcctgc tcgtaatgcg acagcgacat
     9841 cgtccacgcg ccgtgcccgc ccgtcatcga cttcagccgc gcggcgtaac cgtcgagctc
     9901 ggcgagcggc gcgtagccgt tgacggccag catgttcgcc tgcagcgtct gcgtcccggt
     9961 gacctggccg cgccgggagg aaaggtcgcc cgcgacgtcg cccatcgtgt tcgcgggaca
    10021 gttgatctcc atgccgacga tcggctcgag cacgatcggc cgcgccttcg tgatcgcgtc
    10081 gaggaatgcc ttgcggccgg cggcgacgaa cgcgacttcc ttcgagtcga ccgggtggta
    10141 cttgccgtcg tagacgatga cgcgcacgtc ctgcatcgga aatcccgcca ccggtcccga
    10201 cgtcagcacc tgctccacgc ccttctgcac cgcggggatc agcgtgttcg gaatcgcgcc
    10261 gcccttcacc tggtcgacga actcgaaccc ggcaccgcgc ggcatcggct cgatgcgcag
    10321 gtagacctcg ccgaactgtc cggcgccgcc ggtctgcttc ttgtgccgat gatgaccctc
    10381 ggccttcgac gtgaccgtct cgcgatacgg aacgcgcggc ggccgcgtct cgacctcgag
    10441 cttgaactgt gtggccatcc gctcgaacac cgagcgcagg tgcagttcgc caaggccgcg
    10501 cagcaccgtt tcgttgacga tcgcgtcgtg ggcgacgacg agcgtcggat cctcggcgat
    10561 catcttgtgc agcacgtcgg agatgcgctg ctcgtcgccg cggcgcttcg gcgtgatggc
    10621 gaagccgtgc atcggcaccg gaaattcgag cggccgcata tggaggtggt cctcgtcgtg
    10681 cgaatcgtgc agcacgcagt cgaacacgat ctcctccact ttcgcgatcg ccgcgatgtc
    10741 gcccgggacg gcacggtcga gctcgatgta tttcgcgccc tgcagcatga aaagatgccc
    10801 gaccttgaac ggcttgcgcc cgtcgccgac gaagagctgc gtgtcgcgcg tcaccgttcc
    10861 ctgatgcacg cggaacacgc cgagcttgcc gacgaacggg tccatcacga ccttgaagac
    10921 atgcgcgagc acgtgcttcg acggatcggg ctccgagtgg aactcgacgg cgctctcgcc
    10981 ttcgcccttg gtgaagcgcg gcgggttgcc ttccttcgga ttcggcaaca gcttgtcgaa
    11041 gacggtgagc agctcgccga caccggcgcc ggtgcgcgcc gacacgaagc acaccggcac
    11101 cagatgtcct tcgcgcagcg ccttctcgaa tggcgcgtgc aactgttcgg gcgcgatctc
    11161 gccctgctcg aggtagagcg acatcagcgc ctcgtcgact tcgacgacct gatcgaccag
    11221 cgcgctgtgc gccgcggcga ccgatgaaaa atcggcttcg cccgacggag tgaagaaaca
    11281 gtcgacgacg cgccggccgc cgtc
//
LOCUS       DASSVX010000220        20286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1109006, whole genome shotgun sequence.
ACCESSION   DASSVX010000220 DASSVX010000000
VERSION     DASSVX010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 20286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20286
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109006"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VFO53_09475"
     CDS             <1..1404
                     /locus_tag="VFO53_09475"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET8876545.1"
                     /translation="QSAAAIAALVAVDAEARPAAVPHAAAQALRPAQLSDVDHIIVLM
                     KENRSFDHYFGTLAGVRGFDDRSAMTLPDGASVFRQPDARHPDGYLLPYRLDTQRTGA
                     QRLQDLSHSWRALHECWNGGAMDAWVRTHRAISGDAGPLTMGFHTRQDLPFYHALADA
                     FTICDGYHASMFGPTHPNRLFLMTGSIDADARFGAPVTMNGRRDLGWETYPERLERAG
                     ITWRIHHDWDDYGCNMVSYFSAYEHAAKRSPLRDHALRARSHYELLHDIATGNLPQVT
                     WIVPPSTVSEHPDFLPAAGEDHTRSVLMALWSNRALWAKSVVIINYDENDGQFDHVLP
                     PTPPPGTPGEFVNGQPVGLGFRVPCLVVSPFSRGGYVVSDTFDHTSTLRLIETRFGVE
                     VPYLSRWRRETCGDLTATLGLGVPPRIDLPRLPATGARLATVERSVLDLPAPQVPSVQ
                     TMPHQEPGTRKRRGAAG"
     gene            complement(1409..1531)
                     /locus_tag="VFO53_09480"
                     /pseudo
     CDS             complement(1409..1531)
                     /locus_tag="VFO53_09480"
                     /inference="COORDINATES: protein motif:HMM:NF022815.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
     gene            complement(1519..5064)
                     /locus_tag="VFO53_09485"
     CDS             complement(1519..5064)
                     /locus_tag="VFO53_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HET8876546.1"
                     /translation="MATPMAVTAAPLRDVSLDDKYELGSGRVFLTGVQALVRLLILQR
                     QRDTRAGLNTGGFVSGYRGSPLGGLDLALWSAQKFLDRARIKFQPGLNEDLAATAVWG
                     TQQVTMYEGANVDGVFAMWYGKGPGVDRSGDVFKHANFAGTSKHGGVLVLAGDDHAAK
                     SSTLPHQSDHQFSAAMMPVLYPSSVQEILDLGLHGWAMSRYSGCWVGFKCVSDTVESS
                     ASVDGDARDVQIIIPDDFPLPPDGVSIRWPDAFLATEARMQDYKIYAALHYCRVNQLN
                     RIVIDSPNPRLGIITSGKSYLDVRQALADLGITDADAAEIGIRVFKVAMPWPLEPDGV
                     RHFAEGLDEILVVEEKRQIVEYQLKEQLYNWRDDVRPRVVGKFDEKGEWVRPHGDWLL
                     PALSELTPAMIARVIAQRIERLELHPRTIEKIRRRVDFINQKEHALATPKVSLARTPY
                     FCSGCPHNTSTKVPEGSRATAGIGCHVMAIWMDRSTSTFTHMGGEGVPWIGQAPFTDE
                     KHVFANLGDGTYYHSGILAIRAAVAAGVNITYKILYNDAVAMTGGQPVDGPISPRDIA
                     RQCAAEGVTRIVVVTDEPDKYPASYFDAGIEIHHRDALDTVQQQLRETTGTTILLYDQ
                     TCAAEKRRRRKRGKLADPAKRVVINELVCEGCGDCGVKSNCVSAAPVETEFGRKRTID
                     QSSCNKDYSCVNGFCPSFVTVEGGQLRKPKKADAADFTQLPEPAHPSTAQPYGLLVTG
                     IGGTGVVTIGALLGMAAHLEGKGVSVLDMTGLAQKNGAVVSHVRIADAPEQLHATRIA
                     AGEAKLVLACDILTGVGNEALAKMQKGVTKALVNTSLVMPAQFTREPDLAFPIGSMEQ
                     EIRDAVAPGDAEFLDATKLATGLMGDSIATNLFMVGYAYQRGLLPVSEAAILRAVELN
                     GAAVEANKQSFNWGRLAAIEPAKVVDAAIPAARPESQRLSESLDEVIERRRAFLTDYQ
                     DAAYAARYTGLVANVRSVEAAKQPGATDLTEAVARYYFKLLAIKDEYEVARLYAETDF
                     VDRIASQFEGDYTLRFHLAPPTLNKADAKTGEAKKSTYGPWMLPVFRVLAKLRRFRGG
                     ALDVFGRSDERRRERALIGDYEALVDELLARLTPANYALAVSLASIPEHIRGYGHVKE
                     RHLKDAKAREAALLERFRAAPTTQPEAGVALAAAH"
     gene            complement(5164..6294)
                     /locus_tag="VFO53_09490"
     CDS             complement(5164..6294)
                     /locus_tag="VFO53_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8876547.1"
                     /translation="MTDDCARVLIVDDTPLNLKLLGDLLQAKGYAVATANNGEEALAR
                     LAGEVPDIVLLDVMMPGLSGYDVCRRIRADKATSLLPVVMVTSLDPHEERLRGIEAGA
                     DDFLSKPINQPELLARVKSLLRIKALQDEVQRQTIALREWNVRLEDRVRGQVAELERL
                     SQLKRFFAPAVAEAIVSTGEQSILTPHRREICYVFVDLRGFTAFTDAAEPEEVQNVLG
                     DYHAAMGRLIAEHEGTLDRFAGDGILVFFNDPFPIPEPGKRAATMALAMRTRFAELRA
                     RWQKTGYDLDLGMGIARGYATLGAFGYEGRFDYTAVGRAVNLAARLCGEAKPGQILID
                     QRMRAALGDEFTADVIGPMALKGFAQPVQVFALRNQPAGETA"
     gene            complement(6291..6665)
                     /locus_tag="VFO53_09495"
     CDS             complement(6291..6665)
                     /locus_tag="VFO53_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8876548.1"
                     /translation="MSVVLIVEDNEKNMKLVRDVLRVKGYATIEATTAEDGIALAHEQ
                     KPDLILMDIQLPGMNGIDALRVLRTDPATARIPAIAVTASVMQQDRNLITDAGFDGYV
                     GKPINLREFLDAVRTTLELHRT"
     gene            complement(6662..10057)
                     /locus_tag="VFO53_09500"
     CDS             complement(6662..10057)
                     /locus_tag="VFO53_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET8876549.1"
                     /translation="MTRSSANSAYRGRLFRRYLLLIITLVTIALLASGVISVYFTYQE
                     TTAALASLQHEKAIAAATRIEQYINQIQQQLRFAALPQLDASDVELRRIEFLKLLRQA
                     PEVTDVAQLDATGREQIAVSRLGMDSIGSRKDRSGDAAFREAKPGRAWFGPVYFRKET
                     EPYMTIAIRGAGDKGAVTIADVNLKFIWDVVSRIRIGNKGKAYVVDENGFLVADPDIG
                     LVLRKSNLSDLPHVKAAARAINGDYDAAMVSTDLAGTRVLTSVAPIESLKWNVFVEQP
                     VSEVYAKLNAAIVRTALLLLAGLVLSALGALALARGMVRPIRTLAEGAQRIGAGDLDQ
                     QIDIRSGDELQALADQFNRMSGQLRESYAGLERKVDERTHELQNALERQTAMSDILRV
                     ISRSPTDVQPVFDTIAAAALKLCQASSALVFTFDGELIHLAASANVDAQSAAAWHRSF
                     PRPPSRDTAATRSVLTCGVVMIPDVLEDCDYRIGPTAAASGFRSALAVPLIREGKPVG
                     VIGVGGPEPGPFPEEQIALLQTFTDQAVIAIENVRLFNETTEALEQQTAVAEILRIIA
                     SSPGDVRPMLKAVAERALTLCEAAEAGVFLVEGDVLRFATGVGTMAIPEENSTFPLTR
                     GLVVGRAAIDRRTLHYPDVLPYIDSEYPDARPNQERYGFRALLAVPLMREDRAIGAIA
                     LWRREPRAFMPTQIALIKTFADQAAIAIENVRLFNETKEALEQQTAVAEILRVISSSP
                     TDVQPVLDAIAERAAQLCDASAASMYLLDGEVLRHLASKGPSADPVSHVDALPIDRDS
                     VSGRAFLEERAIHVPDMLATGDDYPESRSLAERFGHRTVLVMPLMREGKPFGTILLRR
                     REVRPFTERELALLRTFGDQAAIALENARLFGEIQEKGRQLGVANQHKSEFLASMSHE
                     LRTPLNAIIGFSEVLLERLFGELNDKQDDYLKDIHSSGKHLLSLINDILDLSKIEAGR
                     MDLETSRFDVTLALSNALTLVRERAQRHGIALTQEVDPALGEIVADERKFKQILLNLL
                     SNAVKFTPDGGRIDVTARREDRQAVIAVRDTGIGIATADQALVFEEFRQVGSDYTKKH
                     EGTGLGLALTRRFVELHGGTIHVDSEPGKGSTFTFTIPLEARG"
     gene            complement(10141..11010)
                     /locus_tag="VFO53_09505"
     CDS             complement(10141..11010)
                     /locus_tag="VFO53_09505"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876550.1"
                     /translation="MHPSDLAPPVALRQALRDFGYVEGRNVTYAARWAEARSERLAGL
                     AGELVALKPDLLLTTGWKGAHALKEVTSTIPIVFVGAGDPVRGGLSNSLSRPGGNATG
                     ISDQSVELSAKRLEFLKEADPMAARIAVLWNADDMAMTLRYHEIEKAAQVLGVRVLPL
                     GVREPEDFEQAFSAMTRERPDAFLLVTDALTNLNRRRVLEFAAAHRIPAMYEYAYLVR
                     DGGLISYGPDFDDMYRRAAVYVDRILKGAAPGELAIEQPTRYYLVVNLTTAQSLGLTV
                     PSALLLRADEVIQ"
     gene            complement(11124..12125)
                     /locus_tag="VFO53_09510"
     CDS             complement(11124..12125)
                     /locus_tag="VFO53_09510"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876551.1"
                     /translation="MTDPVGRRRFVWRTLPGALLAAPFLSIAQPPASRVHRIGFLGNS
                     TAALEANLVGPFREGLRDLGYVEGRNLIIEYRWAEGDYKRFPKLIAELASLGVEIIVT
                     AGTPAAQAVQRTAPTMPLVMVAVGDPVGSGLVASLAHPGGRSTGLTSIAPDLEGKRLE
                     LLTRVVPGLAHVAVIWNPDNAFHIGSEREVQAASHVLHFDVLSLPVRVAADFDKAFDA
                     LAAARPGALMVLADRIYLHNRARIVDFAARQRLPAVYAYRELVDAGGLMSFGPDYAEM
                     HRRAAAYVDKILKGANPADLPIEQPTKFRLLINLRTARALGLTIPQSLLLRADELIQ"
     gene            complement(12236..13510)
                     /locus_tag="VFO53_09515"
     CDS             complement(12236..13510)
                     /locus_tag="VFO53_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET8876552.1"
                     /translation="MLFALILAGLVAVLLTGVPVFAGLTLFATAILYAYEGHLGGLGD
                     LVFGELDTYLLVAIPLFTLMAQFMIRGKVVDDLFGAAHTLVRHLPGGLGVATVGACTV
                     FAAISGSSVATALTIGAAAVPLMIRYGYPPRVAYGVVAAGGTLGILIPPSGPMVLYGV
                     VSDTSIGALFIAGVLPGLMLAAIFAVYSMSAARARPREPRASWSEIIAALRRSFWALL
                     LPVLVLGGMYFGVFTATEAAGVGALGALLIAMLVYRNFSLRDLWLSAEDAARNTAMLF
                     MILAAAAILGNVLTKLRIPNQMVDLVLTYGVSQIGFLCAVMALIFVLGMFLETISIIL
                     ITTPVVLPVLAALKIHPVWYGILLTINLELALITPPVGMNLFALKAITRAPLAEIIAG
                     VAPYVLLLIVGLALVMVFPAIALWLPGTMNFR"
     gene            complement(13524..14048)
                     /locus_tag="VFO53_09520"
     CDS             complement(13524..14048)
                     /locus_tag="VFO53_09520"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HET8876553.1"
                     /translation="MSVLERLIRTVARIALGLSAAALLASLATIVYSVVRRYVFNAPV
                     AWSDELVGYLLVASVMLAAAESLLGGEHISVDIVTERLSPRGRWIAFLFGLFAVAATA
                     VLLAVEGVGTVRFSLMVGQRSNGELALPLWMPQALVPIGALLLLAAAAAALVQAWRSK
                     TVPQAPVAPVQGIE"
     gene            complement(14074..15063)
                     /gene="dctP"
                     /locus_tag="VFO53_09525"
     CDS             complement(14074..15063)
                     /gene="dctP"
                     /locus_tag="VFO53_09525"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET8876554.1"
                     /translation="MRFLRSWRIAALFFVAVSSAAAQPAVMRISHQVPPAHHLSKLLQ
                     DFAADVKARTNGSVDVQLFGSEQLAKAGENFPQVARGNIEAAVSVNFQWGTTIPEMSA
                     TLVPYYMTDLAKIRHFVTSDARKLLDQKLEQHGVHSVGWFYITRETIFTSGKKPLVTL
                     DDFKGVKIRGLNSLTDHALTAAGAVTSAMPGSEVYQALQSGVLDAGLTDLSAAYSRKY
                     YEIQKYGTVAPYFTIFFHLYVNPQWWAKLTPEQRQAIEAAAAKTEQEAFDVTEKTAAD
                     ALVQLKAKGMQIHVQTPAEEATWKAAMQKPVIDAFLKSAPSDGAKILELIRGL"
     gene            complement(15108..16004)
                     /locus_tag="VFO53_09530"
     CDS             complement(15108..16004)
                     /locus_tag="VFO53_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HET8876555.1"
                     /translation="MRGTTRLKQLIADPGILVMPGVHDALGARIAEAAGFSAVTVGGY
                     GATATLLGRPDSSQLSLTELSQMYERLCEATTLPILADADTGFGNVTNVARTVSLYEK
                     AGVAGLFIEDQVFPKRCGHMPGKAVVARTEWLAKIKAALDARRDPDLVIMARTDALAV
                     HGLDDAIERAQLAREAGADLLFVEAPTDVEQMRRICAEIPGPCLANNIESGRSPLLPA
                     QALEQIGYAVVVFPVAATYAVAFALRELFTTIRHTGSSEACLPRLVRFDEFNELVGLS
                     AQREREAAWQRYAEALVAGHDR"
     gene            complement(16100..17167)
                     /locus_tag="VFO53_09535"
     CDS             complement(16100..17167)
                     /locus_tag="VFO53_09535"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876556.1"
                     /translation="MNGSRMTTRRRVLRAAGAAMGGALCARMPASFAADAAAPKGAPL
                     AIGLLRNPVSGLIAVTDEKGWFKAAGVDVSSIVFSGAGGPKLIQAMGGGNIGLGSVSA
                     TAALLAFAAQAVALRIVSIATDPAPAFALLSAPQIDAMPKLAGKRVATTAGTGLHYFL
                     ARALRKFGMSPRDIEFVNLPVGDAQAAFLAQRVDAVVPSLNGRYYIESLRKDTRTLFT
                     HADFTKPPGPTTPFEDYDVFVAPKAVVDASAPALRAFLAAYHGKGVPYLNAPATQDAA
                     IADITRYVNTEQKSPTDFAAMKQQLLASRFFDLAGARAIVASDAFRAGLDDQVRFFVE
                     TKQIAAPIQLDGLIANTLLAG"
     gene            complement(17195..18634)
                     /locus_tag="VFO53_09540"
     CDS             complement(17195..18634)
                     /locus_tag="VFO53_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET8876557.1"
                     /translation="MASRDPQRPNVLFILSDDQGAWAMRCAGNADIVTPALDRIAANG
                     VRFTSFFCTSPVCSPARASLLTGEIPSRHGVQDWVRDGNVGPGRIDYLDGRPLLTDAA
                     AAAGYRCALIGKWHLGASDVPRPGHVTWFAHASGMSPYYDAPMVDERRPCTVARYLTD
                     ELTDRALAFVDDESMRAQPFWLALNYTAPHYPWIDSHPRQYTGLYADCAFASCPQEAP
                     HPFSATGNPATDEGHRRARESLIGYFAAVTAMDAGIGRVLARVDDLGLTTSTLVIFMS
                     DNGMNCGHHGIWGKGNGTRPQNMYDTSVKVPALFMQPGRIAPAVRDDLVGGYDVFPTL
                     LDYLGVADPARRKRPGRSFRALLENGAPAIERDVVVYDEYGPVRMVRTREWKYVHRIA
                     DGPHELYDLAGDPGERTNRFGEPATAAIASALRTRLDRWFAEYVDPLQDAARHAVTGC
                     GQLGRVESNVDAAHAFADVPIGALQVPTR"
     gene            complement(18665..19438)
                     /locus_tag="VFO53_09545"
     CDS             complement(18665..19438)
                     /locus_tag="VFO53_09545"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8876558.1"
                     /translation="MPARLQDRVAIVTGAGAGIGAAIARAFCAEGARVAIADLDTGSA
                     ERTATDIGANAVAMPLDVTNEDRVRAVVAETRRRFGRIDILVNNAGIMRKAYVKDMSE
                     DLWDAVVDVNLKATFLCSKAVLPAMIEAKRGRIINIASIAGKVGEPTASAYSAAKFGV
                     IGFTRSLALEVAQFDILVNAICPGPIPTALGQQGWRDGAQVEGVALDRVMARVGSRSP
                     LRRLGTVEQVAQMALFVASDECDFTTGSAFNVDGGIVMT"
     gene            complement(19439..20131)
                     /locus_tag="VFO53_09550"
     CDS             complement(19439..20131)
                     /locus_tag="VFO53_09550"
                     /inference="COORDINATES: protein motif:HMM:NF019031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HET8876559.1"
                     /translation="MKRSQINAAIELAHQTLRKHGIRLPEYAYWSPDDWRRKAGPQHR
                     RIFDNMLGWACTDFGQGDFDRCGITMFDVRNGTPEPNLGTPYGEKIFVLKPGQRLPFH
                     FHYGKTEDIISYAGGTLLIQLYNANPDETMNETTPGHVYCDGVRKSFEPGQIFEIPHG
                     QSLTIVPRLYHRFWAKEDGAVLAGGEISTISVPKTDNKFTAGGRRFVPIEEDVAPTWL
                     LNVDYDQLREVA"
BASE COUNT         3298 a         6696 c         6928 g         3364 t
ORIGIN      
        1 caatccgctg ccgccatcgc ggcgcttgtc gcggtggacg ccgaagcgcg tccggctgcc
       61 gtgccgcacg ccgcagcgca agcgttgcgc ccggcgcaat tgtcggacgt cgatcacatc
      121 atcgtgctga tgaaggagaa ccgctcgttc gatcattatt tcggcacgct cgcaggcgtg
      181 cgcggcttcg acgatcgctc ggcaatgacg ttgccggatg gcgcatccgt tttccgccag
      241 cccgatgcac ggcatcccga cggctacctg ttgccgtatc ggctcgatac gcagcgtacc
      301 ggcgcgcagc ggttgcagga tttgagccat agctggcgcg cgctgcacga gtgctggaat
      361 ggcggcgcga tggacgcctg ggtgcgcacg catcgcgcga tcagcggcga cgccggcccg
      421 ctcacgatgg gctttcatac gcgccaggat ttgccgttct accacgcgct cgccgatgcg
      481 ttcacgatct gcgacggcta tcacgcgtcg atgttcggcc cgacgcatcc caaccggctg
      541 ttcctgatga cgggctcgat cgacgccgat gcgcgcttcg gtgcgcccgt cacgatgaac
      601 ggccgccgcg acctcggctg ggagacctat cccgagcggc tcgagcgcgc cggcatcacg
      661 tggcggatcc atcacgactg ggacgactac ggctgcaata tggtcagcta cttttcggcg
      721 tacgagcacg cggcgaaacg ctcgccactg cgcgatcacg cgctgcgtgc gcgctcgcat
      781 tacgagctgc tgcacgacat cgcgaccggc aatctcccgc aggtcacctg gatcgtcccc
      841 ccgtcgaccg tcagcgagca ccccgacttc ctgcccgcgg ccggcgagga ccatacgcgc
      901 agcgtgctca tggcgctatg gtcgaatcgg gcgctgtggg cgaaatcggt ggtcatcatc
      961 aactacgacg aaaacgacgg ccagttcgac catgtgctgc cgccgacgcc gccgccggga
     1021 acgccgggtg aattcgtcaa cggacagccg gtgggactcg gcttccgcgt gccatgcctc
     1081 gtcgtgtcgc cgttctcacg cggcggctac gtcgtgagcg acacgttcga tcacacgtcc
     1141 acgctgcgcc tgatcgagac gcgcttcggc gtcgaggtgc cgtacctttc gcgctggcgc
     1201 cgcgaaacct gcggcgacct tacggcgacg ctcgggctgg gcgtgccgcc gcgcatcgac
     1261 ctgccgcggc ttccggccac cggcgcgcgg cttgccaccg tcgagcgcag cgtcctcgac
     1321 ctgcctgcgc cgcaggtgcc atcggtgcag acgatgccgc atcaggagcc ggggacccga
     1381 aagcgccgcg gcgcggcggg ctgatgcgtc atccgtgccg gcgcacgagc gccatggcgc
     1441 gctcgagcgt gcgcttgcgc tcgtcggcga gattgcgtcc ggagcggttg atgtagaact
     1501 ggatgaagcg gttgatgctc aatgcgccgc cgccagcgcg acgcctgcct cgggctgcgt
     1561 cgttggcgct gcgcggaagc gctcgagcag cgccgcttcg cgtgccttcg catccttcag
     1621 atggcgctcc ttcacgtggc cgtagccgcg gatgtgctcg ggaatcgacg cgagcgacac
     1681 cgcgagcgcg tagttcgcag gcgtcagccg cgcgagcagt tcgtcgacga gcgcctcgta
     1741 gtcgccgatc agcgcccgct cgcgccggcg ctcgtcgctg cggccgaaca cgtcgagcgc
     1801 gccgccgcga aagcgccgca gcttcgcgag gacccgaaac accggcagca tccagggtcc
     1861 gtacgtcgac ttcttcgctt cgcccgtctt cgcgtcggcc ttgttgagcg tcggtggcgc
     1921 gagatggaag cgcagcgtgt aatcgccttc gaactgcgac gcaatgcggt cgacgaaatc
     1981 ggtttccgcg tatagccgcg cgacctcgta ttcgtccttg atcgcgagca gcttgaagta
     2041 ataccgcgcc acggcctcgg tcaagtcggt tgcgcccggc tgcttcgctg cttccacgct
     2101 gcgcacgttg gcgacgaggc cggtgtagcg cgcagcgtac gcggcgtcct ggtagtcggt
     2161 gaggaacgcg cggcggcgct cgatgacttc gtcgagcgat tcggaaagcc gctgcgattc
     2221 gggccgggcg gcggggatcg ccgcgtcgac gaccttcgcg ggctcgatgg ccgcgagccg
     2281 gccccagttg aagctctgct tgttcgcctc gaccgcggcg ccgttcagct cgaccgcgcg
     2341 caggatggcg gcttcactga ccggcagcag cccgcgctga tatgcgtagc cgaccatgaa
     2401 caggttcgtc gcgatcgaat cgcccatgag tcccgtggcg agcttcgtcg cgtcgaggaa
     2461 ttcggcgtcg cccggcgcga cggcatcgcg tatttcctgc tccatcgagc cgatcgggaa
     2521 cgcgagatcg ggctcgcgcg tgaactgcgc cggcatcacg agcgacgtgt tgacgagcgc
     2581 cttcgtgacg cccttctgca ttttcgccag tgcttcgttg ccgacgccgg tcaggatgtc
     2641 gcaggcgagc acgagcttcg cttcgccggc ggcaatgcgc gttgcatgca gctgttccgg
     2701 tgcgtcggcg atgcgcacgt gcgaaacgac ggcgccgttt ttctgcgcca ggcccgtcat
     2761 gtcgagcacg gacacgccct tgccctcgag atgcgcggcc atgccgagca gcgcgccgat
     2821 cgtcaccacg ccggtgccgc cgatgccggt gacgagcagc ccgtacggct gcgccgtcga
     2881 cggatgcgcg ggctcgggca gctgcgtgaa atcggccgcg tcggcctttt tcggcttgcg
     2941 caactgtccg ccttcgacgg tgacgaagct cgggcagaag ccgttgacgc acgaatagtc
     3001 cttgttgcac gacgactggt cgatcgtgcg cttgcgcccg aactcggtct cgaccggcgc
     3061 cgccgacacg caattcgact tgaccccgca gtcgccgcag ccttcgcaga cgagctcgtt
     3121 gatcacgacg cgtttcgccg gatcggcgag cttgccgcgc ttgcggcggc ggcgcttttc
     3181 ggcggcacag gtctggtcgt agaggaggat cgtcgtgccg gtcgtttcgc gcaactgctg
     3241 ctgcacggtg tcgagcgcat cgcgatggtg gatctcgatg ccggcgtcga agtagctcgc
     3301 cggatacttg tccggttcgt cggtgacgac gacgatcctg gtgacgcctt ccgccgcgca
     3361 ttggcgggcg atgtcgcgcg gcgaaatggg accgtcgacc ggctgtccgc ccgtcatcgc
     3421 gaccgcgtcg ttgtagagaa tcttgtacgt gatgttgacg ccggcggcga ccgccgcgcg
     3481 gatggcgaga atgccgctgt ggtagtaggt gccgtcgccg aggttcgcga acacgtgctt
     3541 ctcgtcggtg aatggcgcct ggccgatcca cggcacgcct tcgccgccca tgtgcgtgaa
     3601 cgtcgacgtg ctgcggtcca tccagatcgc catcacgtgg cagccgatgc ccgccgtggc
     3661 acggctgcct tcgggcacct tcgtcgacgt gttgtgcgga cagccggagc agaagtacgg
     3721 cgtgcgtgcg aggctcacct tcggcgtcgc gagcgcgtgc tccttctggt tgatgaagtc
     3781 gacgcgccgg cggatcttct cgatggtgcg cgggtgcagc tcgagccgct cgatgcgctg
     3841 cgcgatgacg cgcgcgatca tcgccggcgt gagctcggac agcgccggca gcagccagtc
     3901 gccgtgcggg cgcacccatt cgcccttttc atcgaacttg ccgacgacgc gcgggcgcac
     3961 gtcgtcgcgc cagttgtaga gctgctcctt gagctggtac tcgacgatct ggcgcttctc
     4021 ctcgaccacg aggatctcgt cgagcccttc ggcgaaatgg cgcacaccgt cgggctcgag
     4081 cggccacggc atcgcgacct tgaacacgcg aatgccgatc tcggcggcgt cggcgtcggt
     4141 gatgccgagg tcggcgagcg cctgccgcac gtcgaggtag ctcttgccgc tggtgatgat
     4201 gccgaggcgc ggattgggcg aatcgatgac gatgcgattg agctggttga cgcggcagta
     4261 gtgcagcgcc gcatagatct tgtagtcctg catccgcgcc tcggtggcga ggaacgcgtc
     4321 gggccagcgg atcgacacgc cgtcgggcgg cagcgggaaa tcgtcgggaa tgatgatctg
     4381 cacgtcgcgt gcatcgccgt ccaccgatgc cgagctttcg acggtgtccg acacgcactt
     4441 gaagcccacc cagcagcccg aatagcggct catcgcccat ccgtgcaggc cgagatcgag
     4501 gatctcctgc accgacgacg ggtagagcac cggcatcatc gccgcggaga actggtgatc
     4561 cgactggtgc ggcagcgtcg acgatttcgc cgcgtgatcg tcacctgcga gcaccagcac
     4621 gccgccgtgc ttcgaggttc ccgcgaagtt cgcgtgcttg aacacgtcac cgctgcgatc
     4681 gacgccgggt cccttgccgt accacatcgc gaacacgccg tcgacgttgg cgccctcgta
     4741 catcgtcacc tgctgcgtgc cccagacggc ggtggcggcg aggtcctcgt tgagccccgg
     4801 ctggaacttg atgcgcgcgc ggtcgaggaa cttttgcgcg ctccacagcg caaggtcgag
     4861 gccgccgagc ggcgagccgc ggtagcccga cacgaagccg ccggtgttga gcccggcccg
     4921 cgtatcgcgc tggcgctgca ggatcaaaag gcgcacgagc gcctgcacgc cggtcaggaa
     4981 gacgcggccc gagccgagct cgtatttgtc gtcgagggaa acgtcgcgca ggggtgcggc
     5041 agtgaccgcc atcggtgtgg ccatcgcaag agctccaaga atcgtgttcc ggatcgcgcc
     5101 gcgacgggtg gtcgcagcct cgggtcgggc gattgtgtcg cctgtccgta gacaagacga
     5161 attttacgcg gtttcgcccg ctggctggtt acgcagcgca aacacctgaa ccggctgcgc
     5221 gaagcccttg agcgccatcg gaccgatcac gtcggcggtg aattcgtcgc cgagcgccgc
     5281 acgcattcgc tggtcgatca ggatctgtcc cggcttcgcc tcgccgcaca gccgcgcggc
     5341 gaggttgacc gcgcgtccga ccgcggtgta gtcgaagcgg ccctcgtacc cgaacgcccc
     5401 gagcgtcgcg tagccgcgcg cgatgcccat gccgagatcg aggtcgtatc cggtcttctg
     5461 ccatcgcgcg cgcaactcag cgaagcgcgt gcgcatcgcg agcgccatcg ttgccgcgcg
     5521 cttgcccggt tccggtatgg ggaagggatc gttgaaaaag acaaggatgc catcgccggc
     5581 gaagcggtcg agcgtgccct cgtgctcggc aatcagccgt cccatcgccg cgtggtagtc
     5641 cccgagcacg ttctggactt cctcgggctc cgcggcatcg gtgaaggcgg tgaacccgcg
     5701 cagatcgacg aacacgtagc agatctcgcg gcggtgcggc gtgaggatcg actgctcgcc
     5761 agtcgacacg atcgcctcgg cgacagcggg cgcgaagaag cgcttgagct gcgacagccg
     5821 ctcgagctcg gcgacctgcc cgcgcacgcg gtcctcgagc cggacgttcc attcgcgaag
     5881 ggcgatcgtc tggcgctgca cttcgtcctg cagcgccttg atgcgcaaga gcgacttcac
     5941 gcgcgccagc agctcgggct ggttgatggg cttcgacagg aaatcgtcgg cgccggcctc
     6001 gatcccgcgc agccgctcct cgtgcggatc gagcgacgtg accatgacga ccggcaaaag
     6061 cgacgtcgct ttatcggcgc ggatgcggcg gcaaacgtca tagcccgaga gcccgggcat
     6121 catcacgtcg agcagcacga tgtcggggac ttctccggcg agccttgcga gcgcctcttc
     6181 gccgttgttc gccgtcgcga ccgcgtagcc ctttgcctgc agcaggtccc ccagaagctt
     6241 gaggttgagc ggcgtgtcgt cgacgatcaa cacccgggcg cagtcgtcgg tcatgtcctg
     6301 tgcaattcga gcgtcgtgcg cacggcatcg agaaattccc tgaggttgat cggtttcccg
     6361 acatagccgt cgaagccggc gtcggtgatc aggttgcgat cctgctgcat caccgaggcg
     6421 gtgaccgcga tcgccggaat acgcgccgtc gcgggatcgg tgcgcagcac gcgcagcgcg
     6481 tcgatgccat tcatccccgg caactggatg tccatcagga tgagatcggg cttttgctcg
     6541 tgcgcgagcg cgatgccgtc ttcggcggtc gtcgcctcga tcgtggcgta gcccttcacg
     6601 cgcagcacgt cgcggacgag cttcatgttc ttttcgttgt cttcgacgat cagtacgacg
     6661 ctcatcccct ggcctcgagc ggaatggtga aggtgaacgt cgagcccttg ccgggctcgc
     6721 tgtcgacgtg gatcgtgccg ccgtgcaatt cgacgaacct gcgcgtcagc gcgaggccga
     6781 ggcccgtgcc ctcgtgcttt ttcgtgtaat cgctgccgac ctgacgaaac tcctcgaata
     6841 cgagcgcctg atcggcggtg gcgatgccga tcccggtgtc gcgaacggcg atcaccgcct
     6901 gccggtcctc gcggcgcgcc gttacgtcga tgcggccgcc gtccggcgtg aacttgacgg
     6961 cgttcgacag caggttcaac agaatctgct tgaacttgcg ctcgtcggcc acgatttcgc
     7021 cgagcgccgg gtccacttcc tgcgtgagcg caatgccgtg ccgttgcgcg cgctcgcgca
     7081 cgagcgtcag cgcgttcgat agcgcgagtg tgacgtcgaa tcgcgacgtc tcgagatcca
     7141 tgcggccggc ctcgatcttc gacaggtcga ggatgtcgtt gatcagcgag agcaggtgct
     7201 tgcccgaaga atgaatgtcc ttcaggtagt cgtcctgctt gtcgttgagc tcgccgaaca
     7261 gccgctccag cagcacttcc gaaaagccga tgatggcgtt cagcggcgtg cgcagctcgt
     7321 gcgacatgct cgccagaaat tcggacttgt gctggttcgc aacgccgagc tggcggcctt
     7381 tctcctggat ttcgccgaac agccgcgcat tctcgagcgc gatcgcggcc tgatcgccga
     7441 acgtgcgcaa aagtgcgagc tcgcgctcgg tgaagggccg cacttcgcga cggcgcagca
     7501 gaatcgtccc gaacggcttt ccctcacgca tcagcggcat cacgagcacc gtgcggtgcc
     7561 cgaagcgctc ggcgagcgag cggctctccg gatagtcgtc accggtggcc agcatgtcgg
     7621 ggacgtggat cgcacgctct tcgaggaacg cgcggcccga gacggagtcg cggtcgatcg
     7681 gcagtgcatc cacatggctg accggatcgg ccgaaggccc ttttgacgcg agatgccgca
     7741 gcacctcacc gtcgagcagg tacatcgacg ccgcgctcgc atcgcatagc tgcgccgcgc
     7801 gctccgcgat cgcgtcgagc acgggctgca cgtcggtggg cgagctggaa ataacgcgca
     7861 ggatctcggc gaccgccgtc tgctgctcga gcgcttcctt cgtctcgttg aacaggcgca
     7921 cgttctcgat ggcgatggcg gcctggtccg cgaacgtctt gatgagcgcg atctgcgttg
     7981 gcatgaatgc gcgcggctcg cgccgccaca gcgcgatggc gccgatcgca cggtcctcgc
     8041 gcatcagcgg cacggcgagc agcgcgcgaa agccgtagcg ctcctggttc ggacgggcat
     8101 ccgggtactc gctgtcgatg taaggcagaa cgtccgggta gtggagcgtg cgccgatcga
     8161 ttgcggcgcg gccgacaacg aggccgcggg tcaacgggaa cgtcgaattc tcttcgggaa
     8221 tcgccatcgt gccgacgccg gtggcgaaac gcagcacgtc tccttcgaca aggaaaacgc
     8281 cggcctcggc cgcttcgcag agcgtcagcg cgcgttcggc gaccgctttc agcatcggac
     8341 gcacgtcgcc aggcgagctc gcaatgatgc gcagaatctc ggcaacggcg gtctgctgct
     8401 cgagtgcctc cgtcgtttcg ttgaacaggc gcacgttctc gatcgcaatc actgcctggt
     8461 cggtgaacgt ctgcaaaagc gcgatttgct cctcggggaa cgggccgggt tccggtccgc
     8521 cgacgccgat gacgccgacc ggtttgcctt cgcgtatcag cggaacggcg agggcgctgc
     8581 gaaagccact cgccgccgcc gtcggtccga tccggtagtc gcaatcctcg aggacgtcgg
     8641 gaatcatcac cacaccgcac gtaagcaccg atcgcgtcgc ggccgtgtcg cgactcggtg
     8701 gcctgggaaa cgatcggtgc caggcggcag cgctttgcgc atcgacgttc gcactcgcgg
     8761 caagatgaat gagctccccg tcgaacgtga agaccagcgc cgagctcgcc tggcacaact
     8821 tgagcgcagc tgctgcaatc gtgtcgaaca ctggctgaac gtcggtcggc gagcggctga
     8881 tgacgcgcag gatatcgctc attgcggtct gccgctcgag cgcgttctgc aactcgtgcg
     8941 tgcgctcgtc gaccttgcgc tcgaggccgg catacgattc gcggagctgt ccgctcatgc
     9001 gattgaactg atcggcgagc gcctgcagtt cgtcgccgct gcggatgtcg atctgctggt
     9061 cgagatctcc cgcgccgatt cgctgcgcac cttccgccag cgtgcgaatc ggccggacca
     9121 tgcctcgggc gagggcgagc gcgccgagcg cagacagcac gaggcccgcg aggagcagga
     9181 gtgccgtgcg gacgatcgcc gcgttgagct tcgcgtagac ctcggatacc ggctgctcga
     9241 cgaacacgtt ccacttgagc gattcaatcg gcgcgaccga cgtcagcacg cgcgtgccgg
     9301 ccaggtccgt cgacaccatc gcggcgtcgt agtcgccatt aattgcgcgt gccgcggcct
     9361 tcacgtgcgg caggtccgag agattgctct tgcgcaggac gagaccgata tcgggatcgg
     9421 cgacaaggaa gccgttctcg tcgacgacgt aggccttgcc cttgttaccg atgcggattc
     9481 gcgacacgac gtcccagatg aacttcaggt tgacatcggc gatcgtcacg gcgcccttgt
     9541 cgccggcgcc gcggatcgca atcgtcatgt agggctcggt ctccttgcgg aagtacacgg
     9601 gcccgaacca tgcccgaccg ggtttcgctt cgcgaaacgc cgcatccccc gagcggtcct
     9661 tgcgcgaacc gatgctgtcc atgccgagcc gcgaaaccgc gatctgctcg cggcccgtcg
     9721 cgtcgagttg cgcaacatcg gtgacttcgg gcgcctggcg taaaagcttc aggaactcga
     9781 tgcggcgcag ctcgacgtcg ctcgcgtcga gttgcgggag tgccgcaaaa cgcagttgct
     9841 gctggatctg attgatgtac tgctcgatgc gcgtcgccgc cgcgatggct ttttcatgct
     9901 gcaggctcgc aagcgccgcg gttgtttcct ggtaggtgaa gtacacgctg atcacgcctg
     9961 atgcgagcag cgcgatcgtc acgagcgtga tgatgaggag cagatagcgc cggaagaggc
    10021 gtccccgata cgcgctgttc gcgctgcttc gggtcaccgg ggcagcaggc aggggaggca
    10081 tcaccgcggc gctgcccgat gcgagggcgg aattcgtgat tcgaggcagc tccgcggcac
    10141 tcactggatc acctcgtcgg cgcgcagcaa cagcgcgctc ggcacggtga gcccgagcga
    10201 ttgcgcggtc gtcaggttga cgacgaggta gtagcgcgtc ggttgctcga tggcgagctc
    10261 gccgggcgca gcgcctttga gaatgcggtc gacgtacacg gcggcacggc gatacatgtc
    10321 gtcgaaatcc ggcccgtacg aaatgaggcc accgtcgcgg accagatacg cgtattcgta
    10381 catcgccggt atgcgatggg ccgccgcgaa ttcgagcacg cgcctgcggt tgaggttggt
    10441 cagtgcgtcg gttaccagca agaaggcgtc gggccgctcg cgcgtcatcg ccgagaacgc
    10501 ctgctcgaag tcctccggct cgcgcacgcc gagcggcagg acgcgtacgc cgagcacctg
    10561 cgcggctttt tcgatctcgt ggtagcggag cgtcatcgcc atatcatcgg cgttccagag
    10621 cacggcgatg cgtgcggcca tcgggtccgc ttccttgagg aactcgagtc gcttggcact
    10681 gagctccacg gactgatcgc tgatcccggt cgcgttgccg ccgggccgcg acagactatt
    10741 gctgagtccc ccgcgcaccg gatcgccagc gcccacgaac acgatcggga ttgtcgacgt
    10801 cacttccttc agcgcatgcg cgcccttcca cccggtggtt aggagcaggt cgggtttgag
    10861 cgcgacgagc tcaccggcaa gacccgcgag ccgttcgctt cgtgcctcgg cccagcgggc
    10921 ggcgtacgtg acgttgcggc cctcgacgta gccgaagtcg cgaagcgcct ggcgcagcgc
    10981 gaccggcgga gccagatcgg acggatgcac accagccccg agatacgcga tgtgccaggc
    11041 cttggctgat gactgcgcga gactccggat gggggcggcg agaagtgcgc ctccaagcat
    11101 ggcaaggacg tgccggcggc ccgtcactgg atcagctcgt ccgcccgcag cagcagcgat
    11161 tgcgggatcg tgagtcccaa cgcgcgggcg gtgcgcaggt tgatcagcaa gcggaacttc
    11221 gtcggttgct cgatcggcaa atcggcggga ttggcgcctt tgagaatctt gtcgacatag
    11281 gcggccgcgc gccggtgcat ttcggcgtag tccgggccga acgacatgag gccgcccgca
    11341 tcgaccagtt cgcgataggc gtagacggcc ggcaaccgct gtcgcgccgc gaaatcgacg
    11401 atgcgcgcgc ggttgtgcag gtagatgcga tcggccagca ccatcagcgc gccgggccgg
    11461 gcggcggcca gcgcgtcgaa tgccttgtcg aaatcggcag ccacgcgcac gggaagcgac
    11521 agcacgtcga agtgcagcac gtgcgaagcc gcctgtactt cgcgctccga accgatgtgg
    11581 aacgcgttgt cgggattcca gatcaccgcg acatgcgcga gacccggcac cacgcgcgtg
    11641 agaagctcga gccgcttgcc ttcgaggtcc ggggcgatgg atgtcagtcc ggtcgacctt
    11701 ccgccgggat gggcaaggct tgcgacaagg ccgctgccga ccgggtcgcc gacggcgacc
    11761 atcacgaggg gcatcgtcgg ggcggtgcgc tggaccgcct gtgccgccgg cgtgccggcg
    11821 gtcacgatga tctcgacgcc gagactggcg agctcggcga tgagcttcgg gaaccgcttg
    11881 tagtcgccct cggcccagcg atattcgatg atgagattgc ggccctcgac atagccgaga
    11941 tcgcgcagtc cttcgcgaaa cgggccgacg aggttggctt cgagcgccgc cgtcgaattg
    12001 ccgaggaaac cgatgcgatg gactcgcgac gccggaggct gcgcgatcga cagaaacgga
    12061 gctgcaagga gcgcgccggg cagcgttctc cacacgaagc gtcgccggcc gactgggtcc
    12121 gtcatctccc tgctccccgt gaaggaatgc cttcgaaccg gcgcgggcga gccgacccgt
    12181 gcgatcctgc gacgaatcat attcgggaac cgctcgtttc gctaaccgct cgcgatcagc
    12241 ggaagttcat cgtccccggc agccacagcg cgatcgccgg aaagaccatc acgagcgcca
    12301 gcccgacgat caacagcagc acgtacggcg cgacgccggc gatgatctcc gccagcggcg
    12361 cgcgggtgat cgccttcagc gcgaacagat tcatgcccac cggcggcgtg atcagcgcga
    12421 gctcgaggtt gatcgtgagc agaatcccgt accagaccgg atgaatcttc agtgccgcga
    12481 gcaccggcag cacgacgggt gtcgtgatca ggatgatcga gatcgtctcg aggaacatgc
    12541 cgagcacgaa gatcagcgcc atcaccgcgc agaggaagcc gatctgcgat acgccgtagg
    12601 tcagcacgag gtcgaccatc tggttcggga tgcgcaactt ggtgagcacg ttgccgagga
    12661 tcgccgcggc agccaggatc atgaacagca tggcggtgtt gcgtgccgca tcctcggccg
    12721 acagccacag gtcgcgcagc gaaaagttgc gatagacgag catcgcgatc aaaagcgcgc
    12781 cgagcgcgcc gacgcctgcg gcctcggttg cggtgaatac gccgaagtac atgccgccga
    12841 ggacgagcac cggcagcagc agcgcccaga acgagcggcg cagcgcggcg atgatctcgg
    12901 accacgacgc gcgcggctcg cgcggccggg cccgtgccgc gctcatcgag tagacggcga
    12961 agatggcggc gagcatcagc cccggcagca cgccggcgat gaagagcgcg ccgatcgacg
    13021 tatcggagac gacgccgtac agaaccatcg gtcccgacgg cgggatcagg atgccgagcg
    13081 tgccgccggc ggcgaccacg ccgtaggcga cgcgcggcgg atagccgtag cggatcatca
    13141 gcggcacggc ggcggcgccg atggtgagcg cggtggcgac gctcgagccc gagatcgcgg
    13201 cgaacacggt gcacgcgccg acggtcgcga cgccgagccc accgggaaga tgtcgcacca
    13261 gcgtatgcgc ggcaccgaag aggtcgtcga cgaccttgcc gcgaatcatg aattgcgcca
    13321 tcagcgtaaa gagcggaatg gcgacgagca ggtacgtgtc gagctcgccg aagacgagat
    13381 cgcccaggcc cccgagatgg ccctcgtacg catagaggat ggcggtggcg aacagcgtga
    13441 gtcccgcgaa caccgggacg ccggtcagga ggacggcgac gagtccggcg aggatcagcg
    13501 cgaagagcac gagcccggcg tcgctactcg atgccctgca ccggcgcgac cggcgcttgc
    13561 gggacggtct tcgagcgcca cgcctgcacg agcgcggcgg cggcggccgc gagcaacagc
    13621 agcgcgccga tcggcaccag cgcttgcggc atccacagcg gcagagcgag ctcgccattc
    13681 gagcgctgtc cgaccatcag cgaaaagcgc acggtgccga caccctcgac ggcgagcagg
    13741 accgcggtcg cggcgacggc gaaaagaccg aacaggaacg cgatccagcg gccgcgcggc
    13801 gacagacgct cggtgacgat gtcgaccgag atgtgctcgc cgccgaggag cgattcggcg
    13861 gccgcgagca tcacgctcgc gaccagcagg tagccgacga gctcgtcgct ccatgcgacc
    13921 ggtgcattga acacgtagcg gcgcacgacc gaatagacga tcgtcgccag cgacgcgagc
    13981 agcgccgccg cggaaagacc gagcgcaatc ctcgcgaccg tccggatcaa ccgctcgagt
    14041 accgacatca agtgcgagct cggccggctg cgatcacagc ccgcggatca gctcgaggat
    14101 cttcgcgccg tccgacggtg ccgatttcag gaacgcgtcg atgaccggct tttgcatcgc
    14161 cgccttccac gtcgcctcct cggcgggtgt ctgcacgtgg atctgcatgc ccttggcctt
    14221 gagctgcacc agcgcgtcgg ccgcggtttt ttcggtcacg tcgaaggcct cctgctcggt
    14281 cttcgccgcc gccgcctcga tcgcctggcg ctgctcgggc gtgagcttcg cccaccactg
    14341 cggattcacg tacaggtgga aaaagatcgt gaagtagggc gccaccgtgc cgtacttctg
    14401 gatctcgtaa tacttgcggc tgtacgcggc cgacagatcg gtcaggcccg cgtcgagcac
    14461 gcccgactgc agcgcctggt acacctcgga gcccggcatt gccgacgtga ccgcgccggc
    14521 ggccgtgagc gcatggtcgg tcagtgagtt gaggccgcgg atcttcacgc ccttgaagtc
    14581 gtcgagcgtg acgagcggct tcttgcccga cgtgaagatc gtctcgcgcg tgatgtagaa
    14641 ccagccgacg ctgtgcacgc cgtgctgctc gagcttctgg tcgaggagct tgcgggcatc
    14701 ggacgtcacg aagtggcgga tcttcgcgag atcggtcatg tagtacggaa ccagcgtcgc
    14761 gctcatctcg ggaatcgtcg tgccccactg gaagttcacg ctgaccgcgg cttcgatgtt
    14821 gccgcgcgcg acctgcggga agttctcgcc cgccttcgca agctgctccg agccgaaaag
    14881 ctgcacgtcg accgacccgt tcgtgcgcgc cttcacgtcg gcggcgaagt cctgcaacag
    14941 cttcgacagg tgatgcgcgg gcggtacctg atgcgagatg cgcatgacgg cgggttgtgc
    15001 cgctgcggac gacacggcga cgaagaacaa ggcggcgatg cgccaggaac gaaggaaacg
    15061 catggtgtgc gactccggca gggggaaaag gaagaccagg acccgtttca gcgatcgtgt
    15121 ccggcgacga gcgcctcggc gtagcgctgc cacgccgcct cgcgctcgcg ttgcgcggaa
    15181 aggccgacga gctcgttgaa ctcgtcgaag cgaacgagcc ggggaaggca cgcctcgctc
    15241 gagccggtgt gccggatcgt cgtgaacagc tcgcgcagtg cgaaggcgac cgcgtaggtc
    15301 gcggccacgg gaaacacgac gaccgcatag ccgatctgct cgagcgcctg cgccggcagg
    15361 agcggcgagc ggccgctttc gatgttgttc gcaaggcagg gaccgggaat ctccgcgcag
    15421 atgcggcgca tctgctcgac gtcggtcggc gcctcgacga acaggaggtc ggcgccggcc
    15481 tcgcgcgcca gttgcgcgcg ctcgatcgcg tcgtcgagtc cgtgcacggc gagcgcatcg
    15541 gtgcgcgcca tgatgacgag gtcggggtcg cgccgcgcgt cgagcgccgc cttgatcttc
    15601 gcgagccact cggtgcgcgc gaccaccgcc ttgcccggca tgtggccgca gcgcttgggg
    15661 aacacctggt cctcgatgaa aaggcccgcg acgccggcct tctcgtacag cgacaccgtg
    15721 cgcgcgacgt tggtgacgtt gccgaatccg gtgtccgcgt cggcgaggat cggcagcgtc
    15781 gtcgcctcgc acaatcgctc gtacatctgc gagagctcgg tcagcgacag ttgcgacgaa
    15841 tcgggccggc cgaggagcgt cgccgtggcg ccgtagccgc ccacggtgac cgcggaaaat
    15901 cccgctgcct cggcgatccg cgcaccgagc gcatcgtgca cgccgggcat cacgaggatg
    15961 ccgggatcgg caatcagttg cttgaggcgg gtggtgccgc gcatcgcgtg accgcttggg
    16021 aacggggcgc ggccattgta aacgcggcgg caatcgcgcc tctgccaaac gcgaaccgca
    16081 acgctcgccg cgccagcggt cagcccgcga gcagggtgtt cgcgatgagt ccgtcgagct
    16141 ggatcggcgc tgcgatctgt ttcgtttcga cgaagaagcg gacctggtcg tcgagcccgg
    16201 cacggaacgc atcgcttgcg acgattgcgc gcgcgccggc gagatcgaag aatcggctcg
    16261 cgagcaattg ctgcttcatc gcagcgaagt cggtcggact cttctgctcg gtgttcacgt
    16321 agcgcgtgat gtcggcgatt gccgcgtcct gcgtggccgg tgcattgagg tacggcacgc
    16381 ccttgccgtg ataggcggcg aggaacgcgc gcagcgccgg cgcgctcgcg tcgaccacgg
    16441 ccttcggcgc gacgaagacg tcgtagtcct cgaacggtgt cgtcggcccg ggtggcttcg
    16501 tgaaatccgc gtgcgtgaag agcgtgcgcg tgtccttgcg caggctttcg atgtagtagc
    16561 ggccgttgag cgacggcacc accgcatcga cgcgctgcgc gagaaacgcc gcctgagcgt
    16621 cgccgaccgg caggttgacg aactcgatgt cgcgcgggct catgccgaac ttgcgcagcg
    16681 cccgcgccag gaagtagtgc aggccggtgc cggccgtcgt cgcgacgcgc ttgcccgcga
    16741 gcttcggcat cgcgtcgatc tgcggcgccg acagcagtgc gaatgcgggc gccgggtcgg
    16801 ttgcgatcga gacgatgcgc agcgcaacgg cttgcgccgc gaatgcaagc agcgccgcag
    16861 tcgcggagac cgagccgaga ccgatgttgc cgccgcccat cgcctggatc agtttcgggc
    16921 cacccgcgcc gctgaaaacg atcgacgaca cgtcgacgcc cgcggccttg aaccagccct
    16981 tctcatcggt gaccgcgatc agcccggaca ccggattgcg cagcagaccg atcgcaagcg
    17041 gcgcgccctt cggcgccgcg gcgtccgccg cgaagcttgc aggcatccgc gcgcacaacg
    17101 cgccgcccat tgccgcgccg gccgcgcgca acacgcggcg gcgcgtcgtc atccgcgatc
    17161 cattcatggt tgctcctttc gcatggcgcc gtgatcagcg cgtggggacc tgcagcgccc
    17221 cgatcggcac gtcggcgaag gcgtgcgccg catcgacgtt cgattccacg cgacccaact
    17281 gtccgcagcc agtcaccgca tggcgcgcgg catcctgcag cggatcgacg tattcggcga
    17341 accagcgatc gagccgggtg cgaagggccg atgcgatcgc cgcggtcgcg ggttcgccga
    17401 atcggttcgt tcgctcgccg gggtcgccgg cgaggtcgta gagctcgtgc ggtccgtccg
    17461 caatgcgatg cacgtatttc cattcgcgcg tacgcaccat ccggaccgga ccgtactcgt
    17521 cgtagacgac gacatcgcgc tcgatggccg gcgcgccgtt ctcgagcagt gcgcgaaagc
    17581 ttctgccggg acgcttgcgc ctcgccggat cggcgacgcc gagataatcg agcagcgtcg
    17641 ggaacacgtc gtagccgccg acgaggtcgt cgcgcaccgc gggtgcgatg cggccgggct
    17701 gcatgaagag cgcgggcacc ttcaccgagg tgtcgtacat gttctgcggc cgcgtgccgt
    17761 tgcccttgcc ccagatgccg tgatggccgc aattcatgcc gttgtcgctc atgaaaatga
    17821 cgagcgtgct cgtggtcagt ccgagatcgt cgacgcgggc gagcacgcgg ccgatgccgg
    17881 cgtccatcgc ggtgaccgcg gcgaaatagc cgatcaggct ttcgcgtgcc cgccggtggc
    17941 cttcgtcggt ggccggattg ccggtggccg aaaacggatg cggcgcttcc tgcgggcacg
    18001 acgcgaacgc gcaatcggcg tagagcccgg tgtattggcg cggatgcgag tcgatccacg
    18061 ggtaatgcgg tgccgtgtag ttgagcgcga gccagaacgg ctgcgcgcgc atcgactcgt
    18121 cgtcgacgaa cgcgagcgcc cgatcggtca gttcatcggt caggtagcgc gcaaccgtgc
    18181 acggccgccg ctcgtcgacc atcggcgcgt cgtagtacgg gctcatgccc gatgcgtgcg
    18241 cgaaccacgt cacgtgtccg gggcgcggga cgtcgctcgc cccgagatgc catttgccga
    18301 tcagcgcgca gcgatagccg gcggccgcgg cggcgtcggt caatagcggc cggccgtcca
    18361 gatagtcgat gcgtccgggt ccgacgttgc cgtcgcggac ccagtcctgc acgccgtgcc
    18421 gcgacggaat ctcgccggtg agaagcgacg cacgcgccgg cgagcacacg ggcgacgtgc
    18481 agaagaacga cgtgaagcga acgccgttcg ccgcgatgcg atcgagcgcc ggcgtgacga
    18541 tgtcggcatt gcccgcgcag cgcatcgccc atgcgccctg gtcgtcgctc aggatgaaga
    18601 ggacgttcgg gcgctgcggg tctcgactgg ccatcgaatg ttcgtcgcgt tcgttatgcg
    18661 tgcgtcaggt catcacgatg ccaccgtcga cgttgaacgc cgagccggtc gtgaaatcgc
    18721 attcgtcgga ggcgacgaag agcgccatct gcgcaacctg ctcgacggtg ccgagccggc
    18781 gcagtggcga acgcgagccg acgcgcgcca tgacccggtc gagcgcgacg ccctcgacct
    18841 gcgcgccgtc gcgccatccc tgctggccga gcgccgtcgg aatggggccg gggcagatcg
    18901 cgttcaccag gatgtcgaat tgcgcgacct cgagagcgag cgagcgcgtg aagccgatca
    18961 cgccgaactt cgccgcgctg taggcgctcg ccgtcggctc gcccaccttg ccggcgatcg
    19021 aggcgatatt gatgatgcgt ccgcgcttcg cctcgatcat cgccggcagc accgccttgc
    19081 tgcacaggaa cgtcgccttg aggttgacgt cgacgacggc atcccacagg tcctcggaca
    19141 tgtccttcac gtacgccttg cgcatgatgc cggcgttgtt gacgaggatg tcgatgcgcc
    19201 cgaagcgccg ccgcgtctcc gcgacgaccg cgcgcacccg atcctcgttc gtcacgtcga
    19261 gtggcattgc aacggcgttc gcgccgatat cggtcgcggt gcgttccgcg ctcccggtgt
    19321 cgaggtcggc aatggcgacg cgcgcgcctt cggcgcagaa cgcacgcgcg atcgcggcgc
    19381 cgatgcccgc acctgcgccg gtgacgatcg caacgcggtc ttgcagtctt gccggcattc
    19441 aggcgacctc gcgcagctgg tcgtaatcga cgttcagcag ccaggtgggc gccacgtcct
    19501 cctcgatggg tacgaagcgg cggccgccgg cggtgaactt gttgtcggtc ttcggcaccg
    19561 agatggtcga gatctcgcca cccgccagca ccgcgccgtc ctccttcgcc cagaagcgat
    19621 ggtagagccg cggcacgatc gtcaggctct gcccgtgggg gatctcgaag atctgccccg
    19681 gctcgaagga cttgcgcacc ccgtcgcaat acacatggcc cggcgtggtc tcgttcatcg
    19741 tctcgtcggg attcgcgttg tagagctgga tcagcagcgt gccccccgcg tagctgatga
    19801 tgtcttcggt cttgccatag tggaagtgga acggcaggcg ctgcccgggc ttcagcacga
    19861 aaatcttctc gccgtagggc gtgccgaggt tcggctcggg cgtgccgttg cgcacgtcga
    19921 acatcgtgat gccgcagcga tcgaagtcgc cctggccgaa gtcggtgcac gcccagccga
    19981 gcatgttgtc gaaaatgcgc cgatgctggg gacccgcttt gcggcgccag tcgtccggcg
    20041 accagtaggc gtactccggg agacggatgc cgtgcttgcg cagcgtctgg tgcgcgagct
    20101 cgatggctgc attgatctga gatcgtttca tggcggggtc ccggtgagca tgcgaaggga
    20161 aaggcagggg ttggcgcggc gcacgttgcg ctcgaagatg gcatcgatct cctcgcgcgt
    20221 cgcaccacgc cgcgcgagga agtcgtcgac ggtgttcacc caatcggcat acgacgcgaa
    20281 gcgcac
//
LOCUS       DASSVX010000221         7878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_725809, whole genome shotgun sequence.
ACCESSION   DASSVX010000221 DASSVX010000000
VERSION     DASSVX010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725809"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFO53_09555"
     CDS             complement(<1..482)
                     /locus_tag="VFO53_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8876560.1"
                     /translation="MAAATSSVDDADVFVDVQNVRLNYKVGDDSSTLALDQASMTLRR
                     GEFVAIVGPSGCGKSSLLKLASGLRRPSQGEVVVGGSAVVKPIKIVGMAFQNPTMLPW
                     LRTIDNVLMPLRIVEPHRSQFRAKKDAYRAMAQSLLGEVGLGNFADKYPWELSGGMLQ
                     R"
     gene            complement(497..1543)
                     /locus_tag="VFO53_09560"
     CDS             complement(497..1543)
                     /locus_tag="VFO53_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876561.1"
                     /translation="MSVRAFLRIAAAALAALTVATAAHAADPVKIRFVLNWKYQGPQA
                     WFFVAQDKGYFKAEGLDVQMDQGEGSGAAVTRVASKAYDMGFGDINALTVFAAQHPED
                     SPKAVFMVYGNSPFSIYSLKDKGFTSPKQLEGKTLGAPVDDAPFKLFPAFAKAAGIDA
                     SKVKWKHMQPSLREQMLVRGEVDAVSGYINTVWFSLRKIYPNPGDKVNIMKYSDYGLD
                     LYSNAIIVSKDFIQAHPQAVAGFLRAIAKGFQDVLANPGMGVDAALKRQPLLDRRIEL
                     ENLIATVNIEMLSPESKRIGVGDVDDAHLKKGIGIVVDAYELKRTPAPDEVFTRQFLP
                     ARELRNIHELKTPE"
     gene            complement(1585..2148)
                     /locus_tag="VFO53_09565"
     CDS             complement(1585..2148)
                     /locus_tag="VFO53_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET8876562.1"
                     /translation="MKVAVFCGSNPGRSQVYGDAALKLGRALAARGVELVFGGTNKGL
                     MKIVADAVVAGGGAVHGVIPAALVDKGQQYSQLTHADIVETRSLRKRRMAEIADGFIA
                     MPGGVGTLEELLEMWVDAQLDGHGKPLGLLNVGGFFDGLLTFVDTMIDEGFLPPQHRD
                     MIIAMPDPENLLDAFRSFAPVTTSKWM"
     gene            complement(2213..2632)
                     /locus_tag="VFO53_09570"
     CDS             complement(2213..2632)
                     /locus_tag="VFO53_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET8876563.1"
                     /translation="MDVEKHLRLTQAGAMKIVESAVRHAVEMGKPQCIAVVDEGGHLL
                     AFTRMDGGKRLSIESSIRKASTAASSRAPTGGIQGELEFRLAHATSGQLVNLKGGLPI
                     IVDGITIGGIGVGSQTGDEDLEVAKAGLRAIAGAVSG"
     gene            complement(2652..3764)
                     /locus_tag="VFO53_09575"
     CDS             complement(2652..3764)
                     /locus_tag="VFO53_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-opening amidohydrolase"
                     /protein_id="HET8876564.1"
                     /translation="MSRERFESRVIRVPMNSPGDVSGVAALFDRGDVDPAHVVAIMEQ
                     TEGDPYARGYATLAMEVLLSERLGMSRRQVFDTIPMMMIGGVGGIMCPHIILFIRQPG
                     GVAGRGKRLALGVASSRELEPEEYGTMIQVDLVTEAVRDAMRDAGMTHVSQIECVEVK
                     CPAMTPARIADAQMRGAKVANTNPAVASSLAKGACALGVAVATGEVEREKLRDGVVNV
                     DKSLYSSIASTSAGGEQVACRVLVIGNVDGAPGKLVAAHGVMEDQLDIAGARNAFARA
                     GLALHEGSVAERDRERIAAVLVNAGADYVGSVRGRRHVLHSDFLSPFSGIQAKAVAHA
                     IVATIVGDTMFLASAGAEHQGPPGANLVCVIAEAAA"
     gene            3969..4139
                     /locus_tag="VFO53_09580"
     CDS             3969..4139
                     /locus_tag="VFO53_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876565.1"
                     /translation="METDKLLHSEVRVINIGLREFALDLARCHVAVVHVDWSPPAVSD
                     PRISSLLAKLGG"
     gene            4141..5403
                     /locus_tag="VFO53_09585"
     CDS             4141..5403
                     /locus_tag="VFO53_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HET8876566.1"
                     /translation="MTSVTPVDTIAAANREALRRILASEPVLVDVQLAKDAIPQLPER
                     TILHAGPPIGWDRMCGPMRGAVAGAIVFEGWAEDLEAASRLAASGEIRFEPNHHYDAV
                     GPMTGMTTRNMAVLVVENRAFGNRAFCTVNEGIGNVMRFGGNDREVIDRLQWMAAVAG
                     PALGAAIRNRDGVPLKPLIARGLSMGDEMHMRNAACSSLILRELAGELADTATRELGR
                     ILRFVAGNDMFFLNVAMAMAKATVAPAHGIAHSTVVTTMARNGTDFGIRVSGTGDRWF
                     TGPAQPARGLLFAGFTEADANPDIGDSCITETVGLGGFAMAASPAVVGFIGAGTLADA
                     IGYTRSMREITLGTNADWALPTLDMTGVPTAIDIRKVLATGLLPVINTAIANRLPGRG
                     QIGAGVVHPPLACFEQALVTFAEMNGAN"
     gene            5406..6908
                     /gene="fdrA"
                     /locus_tag="VFO53_09590"
     CDS             5406..6908
                     /gene="fdrA"
                     /locus_tag="VFO53_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HET8876567.1"
                     /translation="MAHVVNQVRRAFYRDSVALMRISRAVGALDGVASAALMIGSVTN
                     KNLMRDAGLLNADGERAGPNDLIFAVSADGEAQAATALAEAERLLDAPAATEQGAGQW
                     HPKSLDTALAQLPGANLALISVPGEFAAAEARRALNRGLHVMMFSDNVPIADEVALKQ
                     DARQRGLLMMGPDCGTAIIGGVPLAFANVIARGDIGLVGASGTGLQEVSTLIARGGGG
                     VSHVIGVGGRDLKQAVGGITTLMAFDALDRDPGTRQIVLISKPPEPAVAKVVLDRIAQ
                     SSKKVSICFIGVDEMPVPPNAALFSDLRSTAQSALSGKRIVWKDQPPAASELAARVAR
                     GRSRVQGLFSGGTMCAEAQVFFRRAGIAVSSNVPIPGVRKAAAGDVHALLDLGDDEYT
                     VGRPHPMIDATLRNRMLSDALEDNRTAVILMDVVIGYGANADPAGELISVLPPANERK
                     AVLITSVCGTEDDPQGYSRQVTLLEDAGVIVAPSNAHAAELAIDVLRKLP"
     gene            complement(6912..>7878)
                     /locus_tag="VFO53_09595"
     CDS             complement(6912..>7878)
                     /locus_tag="VFO53_09595"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HET8876568.1"
                     /translation="VDRPDSAATLELTAPIAVLGNAARTTLRASRHGSVLAVFRRSFY
                     VRFGIDLVCVGPVALGRGPLNALHATIDPVSWLDRGLAPGAEVRSDGRRLAVDDRFVF
                     DFATADVWQPTVAPPSAIASLHAGVQLLAQAARQRSPGGLGALLWLSANELDNDARGA
                     RDPLLRAAMPPVAALRGWLASVLADVDAPLPAIGALIGLGPGLTPSGDDFLCGTMAAL
                     HYLGRAGVASRLAAIVLPRLPETNLISAAYLRCASTGDASSVLFDVLDCLSGVDSALL
                     AQRLDAVDRVGHTSGWDCLAGAAAVCAELATLLPRHAHAAAHAGC"
BASE COUNT         1324 a         2670 c         2596 g         1288 t
ORIGIN      
        1 cgctgcagca tcccgcccga cagctcccac ggatacttgt cggcgaaatt gccgagcccc
       61 acttcgccaa gaagcgactg cgccatcgcg cgatacgcgt ccttcttcgc gcggaactgg
      121 ctgcggtgcg gctcgacgat ccggagcggc atcagcacgt tgtcgatcgt gcgcagccag
      181 ggcagcatcg tgggattctg gaacgccatg ccgacgatct tgatcggctt cacgactgcg
      241 cttccgccga cgacgacctc gccctgcgac ggccggcgca atccggacgc gagcttcagc
      301 agcgacgact tgccgcagcc gctcggaccg acgatcgcga cgaactctcc acgccgcagc
      361 gtcatgctcg cctggtcgag cgccagcgtc gagctgtcgt cgccgacctt gtagttgagc
      421 ctgacgttct gcacgtcgac gaacacgtcg gcgtcgtcga cgctcgaggt cgcggcggcc
      481 actgcgccga ttgtggtcat tccggcgtct tgagctcgtg gatgttacgc agctcgcgtg
      541 ccggcaggaa ctggcgcgta aacacttcgt cgggcgccgg cgtgcgcttc agctcgtacg
      601 catcgacgac gatgccgatg cccttcttca aatgtgcatc gtcgacgtcg cccacgccga
      661 tgcgcttcga ttccggcgag agcatctcga tgttcaccgt ggcgatcagg ttctcgagct
      721 cgatgcgccg gtcgagcagc ggctggcgct tgagtgctgc gtcgactccc atcccgggat
      781 tggcgagcac gtcctggaag cccttcgcga tcgcgcggag gaacccggcg acggcctgcg
      841 gatgcgcctg gatgaagtcc ttcgacacga tgatcgcgtt gctgtaaaga tcgagaccgt
      901 agtcgctgta cttcatgatg ttcaccttgt cgcccgggtt cgggtagatc ttgcgcaggc
      961 tgaaccagac cgtgttgatg tatccggaca ccgcgtcgac ctcgccgcgc acgagcatct
     1021 gctcgcgcag gctcggctgc atgtgcttcc atttcacctt gctggcgtcg atgccggcgg
     1081 ccttcgcgaa tgccggaaag agcttgaacg gcgcatcgtc gaccggtgcg ccgagcgttt
     1141 tgccttcgag ctgcttgggc gaagtgaagc ccttgtcctt cagcgagtag atcgagaacg
     1201 gcgagttgcc gtacaccatg aacacggcct tcggcgagtc ctcggggtgc tgagcggcga
     1261 aaacggtcaa cgcattgatg tcgccgaacc ccatgtcgta cgccttgctc gcaacccggg
     1321 tgacggcggc gcccgatccc tccccctggt ccatctgcac gtcgaggccc tcggccttga
     1381 aatagccctt gtcctgcgcg acgaagaacc atgcctgcgg gccctggtat ttccagttca
     1441 gcacgaagcg gatcttgacc ggatccgcgg catgcgcggc ggttgcaacg gtcagcgcgg
     1501 cgagcgccgc ggcggcgatg cgcagaaagg cacgtacgga catggcggat tctcctcgat
     1561 cggtttgacg gcgacggcgc caggtcacat ccacttggac gtggtgaccg gagcaaacga
     1621 acgaaatgca tcgagcaggt tctcgggatc gggcatggcg atgatcatgt cccggtgctg
     1681 cggcggcagg aagccttcgt cgatcatcgt gtcgacgaac gtcagcaggc cgtcgaaaaa
     1741 tccgccgacg ttgagcagcc cgaggggctt gccatgaccg tcgagctgcg cgtcgaccca
     1801 catttcgagc aactcctcga gcgtgccgac gccgccgggc atggcgatga aaccgtcggc
     1861 gatctcggcc atccggcgct tgcgcagcga ccgggtctcg acgatgtcgg catgcgtgag
     1921 ctgcgaatac tgctgaccct tgtcgacgag cgctgccgga atgacgccgt gcacggcgcc
     1981 gccgccggcc accacggcgt cagcaacgat tttcatcagg cccttgttgg tgccaccgaa
     2041 cacgagctcg acgccgcgcg cggcaagcgc gcggccgagc tttagcgcgg cgtcgccata
     2101 aacttgcgac cggccgggat tcgatccaca aaaaacggcg actttcatga tcgcgggatt
     2161 tccacattgg cccggcctgc agtcgccgaa ccgcctttct tgcgcggacc gctcagccag
     2221 acaccgcacc cgcgatggcg cgaagacccg ccttcgcgac ttcgaggtct tcgtcgccgg
     2281 tctgcgagcc gacaccgatg ccgccgattg tgatgccgtc gacgatgatc ggcaatccgc
     2341 ccttcaggtt gacgagctgc cccgacgtcg catgcgcgag ccggaattcg agctcgccct
     2401 ggatgccccc ggtcggcgca cgactcgacg ccgccgtcga tgccttgcga atcgaagatt
     2461 cgatgctgag ccgcttgcca ccatccatcc gcgtgaacgc gagcaggtgg cccccctcgt
     2521 cgacgaccgc gatgcattgc ggcttgccca tctcgaccgc gtgcctgacg gcggactcga
     2581 cgatcttcat cgcgccggcc tgcgtcagtc gcaaatgctt ttcgacatcc atgacgaata
     2641 tccctcgaac gctacgcggc ggcttcggcg atgacgcaca ccaggttggc acccggcgga
     2701 ccctgatgct cggcgcccgc cgacgcgagg aacatcgtgt cgccgacgat cgtcgccacg
     2761 atggcgtgcg cgaccgcctt ggcctggatg ccgctgaacg gcgacaggaa gtcggaatgc
     2821 agcacgtggc gtcgcccgcg aacgctgccg acgtaatcgg cgcccgcgtt cacgagcact
     2881 gcggcgatgc gctcgcggtc gcgctcggcg acgctgccct cgtgcagtgc gagccccgcc
     2941 cgcgcgaacg cgttgcgcgc accggcgatg tcgagctggt cctccatcac gccgtgcgcg
     3001 gcaacgagct tccccggcgc gccgtcgacg ttgccgatga ccagcacgcg acacgccacc
     3061 tgctcgcctc ccgccgacgt cgaggcaatc gacgaataca gcgatttgtc gacattgacg
     3121 acgccgtcgc gcaacttctc gcgttcgacc tcgccggtgg ccacggcaac acccagcgcg
     3181 cacgcccctt tggcgagcga cgacgcgacg gccgggttcg tgttggcgac cttcgctccg
     3241 cgcatctgcg cgtcggcgat gcgcgcaggc gtcatcgcgg ggcacttgac ctcgacgcac
     3301 tcgatctgcg agacgtgggt catgcccgcg tcgcgcatcg cgtcgcgcac ggcctcggtc
     3361 accagatcca cctggatcat cgtcccgtat tcctcgggct cgagctcgcg cgagctggcg
     3421 acgccgagtg caaggcgctt tccacggccg gcgacgccgc ccggctgccg gatgaagaga
     3481 atgatgtgcg ggcacatgat gccgcccacg ccaccgatca tcatcatcgg tatcgtgtcg
     3541 aagacctgcc ggcgcgacat gccgagccgc tcggacagca gcacttccat cgcgagcgtc
     3601 gcgtagccgc gagcgtacgg gtcaccctcg gtttgctcca tgatcgcgac gacgtgcgcg
     3661 ggatcgacgt cgccgcggtc gaacagcgcc gcgacgcccg aaacgtcgcc gggcgaattc
     3721 atcggtaccc ggatcactcg cgattcgaat ctttccctgg acatttcgtc agcatcccgt
     3781 catgcattgt gcgaacaaac cgcgcgccaa ccgaaacgcc cgcgcagtga cgctccgcgg
     3841 gttccggagc cttcggcgcg ggcgccggcg gcgatcagta aactggctat gcgatgcaat
     3901 gtcaagatat ttcgaaattc ctgccactgt gcgttattat gcgatcaaag cccgcgagga
     3961 gcgaagcgat ggaaaccgac aagctgctgc attccgaggt gcgcgtaatc aacatcgggc
     4021 tgcgcgagtt cgcgctcgac ctggcgcgct gccatgtcgc tgtcgtgcac gtggactggt
     4081 cgccgcctgc ggtgagcgat cccaggatca gcagcctcct ggcgaagctc ggcggctgac
     4141 atgacgagcg tcacccccgt cgacacgatc gccgccgcca accgcgaggc gttgcgacgg
     4201 atcctcgcaa gcgagccggt cctggtcgac gtccagctcg cgaaagacgc gatcccgcaa
     4261 ctgcccgagc ggacgatatt gcatgccgga ccgccgatcg gctgggaccg catgtgcgga
     4321 ccgatgcgcg gcgccgtggc cggcgccatc gtgttcgaag gatgggccga ggacctcgag
     4381 gccgcgagcc gtctcgctgc gtccggcgag atccggttcg agcccaacca tcattacgat
     4441 gcagtcggcc cgatgaccgg gatgacgaca cgcaacatgg cggttctcgt ggtcgagaac
     4501 cgtgcgttcg gcaatcgcgc gttctgcacg gtcaacgagg gcatcgggaa tgtaatgcgc
     4561 ttcggcggca acgatcgcga agtgatcgac cggctgcaat ggatggcggc ggtggccggt
     4621 cctgcgctcg gtgccgcgat acgcaaccgc gatggcgtgc cgctgaaacc cctgatcgca
     4681 cgcggccttt cgatgggcga cgaaatgcac atgcgcaacg ccgcctgctc gagcctgatc
     4741 ctgcgcgagc tcgccggcga gctcgccgac accgcgacgc gcgagcttgg ccgcatcctg
     4801 cgtttcgttg cgggcaacga catgttcttc ctcaacgtcg cgatggcgat ggccaaggcg
     4861 acggtggcgc cggcgcacgg tatcgcgcac tcgaccgtcg tcacgacgat ggcgcgtaac
     4921 ggcaccgatt tcggcattcg cgtcagcggc accggcgatc gctggttcac gggtcccgcg
     4981 cagccggcca ggggtttgct gttcgccggg ttcaccgagg ccgatgccaa tccggacatc
     5041 ggcgattcgt gcatcaccga gacggtcggg ctcggcggct tcgcgatggc ggcgtcgccc
     5101 gccgtcgtcg gcttcatcgg cgcgggaaca ctggccgacg cgatcggtta cacgcggagc
     5161 atgcgcgaga tcacgctcgg caccaatgcc gattgggcgt tgccgacgct cgacatgacg
     5221 ggcgtgccta ccgccatcga catccgcaag gtgctcgcga ccggattgct gcccgtgatc
     5281 aacaccgcga tcgcgaaccg gttgcccgga cgcggccaga tcggcgccgg tgtcgttcat
     5341 cctcccctcg cgtgtttcga gcaggcactc gtcaccttcg ccgaaatgaa cggagcaaac
     5401 tgaacatggc ccacgtcgtc aaccaggtac gccgcgcgtt ctatcgcgac tcggtcgcgc
     5461 tgatgcgcat ctcacgcgcg gtcggcgcgc tcgacggcgt ggcgagcgct gcgctgatga
     5521 tcggctcggt caccaacaag aatctgatgc gcgacgcggg actgctgaac gccgatgggg
     5581 aacgcgcggg tcccaatgac ctgatcttcg cggtatcggc cgacggcgag gcgcaggcgg
     5641 cgacggcact cgccgaagcc gagcgcctgc tcgacgcgcc cgccgcgacc gagcagggtg
     5701 ccggccagtg gcatcccaaa tcgctcgaca ccgcgcttgc gcaattgccg ggcgccaatc
     5761 tcgcgctcat ctcggtgccc ggcgaattcg ccgcggcgga ggcgcgacgc gcgctcaatc
     5821 gcgggctgca cgtgatgatg ttcagcgaca acgtgccgat cgccgacgag gtcgcactga
     5881 agcaggatgc gcggcagaga ggcttgctga tgatgggtcc cgattgcggg acggcgatca
     5941 tcgggggggt tccgctcgcc ttcgccaatg tgatcgcgcg cggcgacatc ggcctcgtcg
     6001 gcgcgtcggg caccgggctg caggaagtat cgacgttgat cgcacgtggc ggtggtggcg
     6061 tgtcgcacgt gatcggcgtc ggtgggcgcg acttgaagca ggcggttggc ggcatcacga
     6121 cgttgatggc tttcgacgcg ctcgaccgcg atcccggcac gaggcagatc gtgctgatct
     6181 cgaagccgcc ggaacccgcg gttgcaaaag tggttctcga tcgcatcgcg caaagcagca
     6241 agaaagtgtc gatctgcttc atcggtgtcg acgagatgcc ggtgccgccg aatgccgcgc
     6301 tgttttccga cctgcgatcg accgcgcaga gcgcgctgtc gggcaagcgc atcgtctgga
     6361 aagatcagcc gcccgccgcg tcggagctcg ccgcacgcgt tgcccgcggg cgctctcgcg
     6421 tgcagggact tttttccggc ggcacgatgt gcgccgaagc gcaggtgttc ttccgccgcg
     6481 ccggcatcgc ggtttcgtcg aacgtcccga tacctggcgt gcgcaaggcc gcggccggcg
     6541 atgtgcatgc gctcctcgat ctcggcgacg atgaatacac ggtcggccgc ccgcatccga
     6601 tgatcgacgc gacgctgcgc aaccgcatgc tgtcggatgc gctcgaggac aaccggacgg
     6661 ccgtgatcct catggacgtg gtgatcggtt acggggcgaa cgcggacccc gccggcgagc
     6721 tcatttccgt gctgccgccg gcaaacgagc gcaaggcggt gctgatcacg tcggtgtgcg
     6781 gaaccgaaga cgacccgcag ggttattcgc ggcaggtcac gctgctcgag gacgccggtg
     6841 tcatcgtcgc gccgtcgaat gcgcacgcgg cggagcttgc gatcgacgtg ctgcgcaagc
     6901 ttccgtagat ttcagcagcc ggcatgcgcc gcagcatgcg catgcctcgg cagcagcgtt
     6961 gcgagctcgg cgcacaccgc cgccgcaccg gcgagacagt cccagcccga cgtgtgcccg
     7021 acgcgatcca ccgcgtcgag acgctgcgcg agcagcgcgc tgtccactcc ggacaggcag
     7081 tcgagcacgt cgaacagcac gctcgacgcg tcgccggtcg atgcgcagcg caagtaggcg
     7141 gcgctgatca ggttcgtctc cgggagtcgc ggcaaaacga tggccgcgag gcgcgaggcg
     7201 acaccggccc gaccgagata gtgcagtgcg gccatcgttc cgcaaaggaa gtcatcgccc
     7261 gatggcgtca atccgggacc gagcccgatc aacgcgccga tcgcggggag cggcgcgtcg
     7321 acatccgcga gcaccgacgc gagccagccg cgaagtgccg cgaccggcgg catcgctgcc
     7381 cgcagcagcg gatcgcgcgc cccgcgcgca tcgttgtcga gctcgttcgc ggataaccac
     7441 agcagcgcac caagaccgcc gggcgagcgt tgccgcgccg cctgcgcgag aagctgcacg
     7501 ccggcatgca gcgacgcgat cgcggaaggc ggagcaaccg tcggttgcca cacgtcggcc
     7561 gtggcgaagt cgaacacgaa gcgatcgtcg accgcgagtc tgcgtccatc ggaccggacc
     7621 tcggcgccag gcgccagacc gcggtcgagc cacgacacgg gatcgatcgt cgcatgaagc
     7681 gcattcaatg gcccgcgtcc gagcgcgacc ggccccacgc acacgagatc gatgccgaag
     7741 cggacgtaga agctgcgccg gaatacagcg agcaccgatc catggcggct cgcacgcaac
     7801 gtcgtgcgtg cggcgttgcc gagcaccgcg atgggcgcag tcaactcgag cgtcgcggcg
     7861 ctgtcgggcc gatcgacg
//
LOCUS       DASSVX010000222        13166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_609303, whole genome shotgun sequence.
ACCESSION   DASSVX010000222 DASSVX010000000
VERSION     DASSVX010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 13166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13166
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609303"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1087)
                     /locus_tag="VFO53_09600"
     CDS             complement(62..1087)
                     /locus_tag="VFO53_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8876569.1"
                     /translation="MGRFDPAAARRIGRRDLAVAQLGVGALPFGSFANDDTDASIDGA
                     FAAAYAAGLRYFDTAPLYGLGLSEHRLGACLRGIDRRSVVLSTKVGRLLRPLEGGPAP
                     GICAGSSPFNVEFDYSYDGTLRSLEASMQRLGTNALDIVLIHDVNRRWQGDLVEQRYR
                     EAMEGAHRALCDLRTAGTIKAFGVGVNDWQILLRFAADGDFDCFMLAGRYTLLDHTSL
                     DTFMPECERRNISVLMAAPFNSGILATGATTGATYFYAEADAEVLSRTKRIEAICARH
                     RVAIAAAALQFPLAHPAVASVVTGMRSRREVDANVAHCQADIPRDFWAELKAEGLVAA
                     GAPVPAV"
     gene            complement(1096..1995)
                     /locus_tag="VFO53_09605"
     CDS             complement(1096..1995)
                     /locus_tag="VFO53_09605"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8876570.1"
                     /translation="MITVGFIGLGTMGAPMARNLLHKGHRLVVADVVPAAVASLTAAG
                     ALAARTPKEVAAASDFVITMLPDAPDVERVALGADGIVAGIRAGSVYIDMSTIDPDTT
                     RNVGEQIASKGASMIDSPVGKTADHAVAGTLTLMVGGPDDVVARCRPILDCMGTDFFH
                     CGGLGAGQTMKLINNLLATAVSEASIEALVTGAKSGLTLDTMMSVLRTTMAWNNALAI
                     ALPKRPLAGDFSPGFMLKLAHKDCRLALEMARRLGVITPVGRAALASLDEAMQRGLHD
                     DDVGALLKLREDAAGVEVRLPPA"
     gene            complement(2081..3322)
                     /locus_tag="VFO53_09610"
     CDS             complement(2081..3322)
                     /locus_tag="VFO53_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876571.1"
                     /translation="MSIEKDRKSVSRRRFIATAGAASLAFAAPAIVRAQGRKALKVSV
                     GRQPWAAGNSPVTKYMMDNKTFERFAGDAGYDLSVDYRDYPSAAPQVEAFVSGNLDFG
                     MWGNTPIVRLVAQNQPIQIMTVGEGHFRFVLATRKDSPIRNVADLKGKTVGALLGGDP
                     YNVLSQMLRYELGNPDPKAFNITVVNTPTQAQAATIPGGMDAAVVIYPAFLKASAELG
                     IVGIINSFGYTESHYKGPAGEGAGILLPSVKKSPFYPDGFYLHRSFWIGSRKIIESDP
                     AVATAFVTAQQDAVAKLTKMDPGTVSELAKKYWELPPELGAKVVEDDVLFKRGWCWPT
                     EGDAVALLETSKSMVDGKLITKPLTWDQVKGAFALAAPLDKAAYEKTGRVPDAAGFHD
                     KNAKDLRGAPVWDMPSWGTRT"
     gene            complement(3349..4179)
                     /locus_tag="VFO53_09615"
     CDS             complement(3349..4179)
                     /locus_tag="VFO53_09615"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876572.1"
                     /translation="MTGATPIAGAKLRLAHVAKHFDVGGAKPTIAVDDFTLDIVQGEF
                     LVIVGPSGCGKTTVLNILAGLERPSAGSVTLDDREITGPGPARGVMFQDYALFPWQTV
                     QGNVGFGLKYGPSGNGLSTDARARRVQDCIDLVGLNGAEDKYPHQLSGGMRQRAALAR
                     LMANEPEVLLMDEPLAALDAQTRLILQDELLRIWGQDRPAARRRTVVFITHAIDEAVF
                     LADRVAVMTTRPGRLKALIDVPLPRPRNDATRASAEFQALSQHIWSLIRDEAYRATVT
                     "
     gene            complement(4176..4946)
                     /locus_tag="VFO53_09620"
     CDS             complement(4176..4946)
                     /locus_tag="VFO53_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876573.1"
                     /translation="MIAARIVRGLGLPLLLLALWQAWGSASHNPRMPVPTHVVTAAVD
                     LLRSGELPLAIMQSLSRVFAGFAVAAVIAIPLGLSMGTFRGVERNVDPLVESFRPIAA
                     IAILPLAILWLGTGTGAAVMIVAYAAFFPIVINTVAGAKGVNATLLRAAATMGVPRLR
                     TIRTVIVPAALPAIGVGLRIAMGVAWTAIVAAELAVGAKAGGGGSGGIGQMMFVFYAY
                     SIELNRIIVCMIAVGLVALVLDRLLRIGMRLLMPWTPR"
     gene            complement(4943..5716)
                     /locus_tag="VFO53_09625"
     CDS             complement(4943..5716)
                     /locus_tag="VFO53_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876574.1"
                     /translation="MRNAFAGYRPALIYARTMLAFFVVWTLVAMLTANAILLPSPLTV
                     AGAMLDLARDLELFLHAGISLGRMAVSLALGSLLAIPLGLAMGLSRALDQIFDPIIEI
                     LRPISGIAWIPLALFIFGIGNALPVFIMTYAGFFPILLGTIAGVRSVDRRLIDAARTM
                     GVPRRTIVTRVVVPAALPSLMVALRLGVASSWTAVVAAELIGAPSGLGYAVEWYRELL
                     ATPQVMSFIAMIGVLGYLCDLGVRALGRWFTPWAREAVR"
     gene            5838..6890
                     /locus_tag="VFO53_09630"
     CDS             5838..6890
                     /locus_tag="VFO53_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET8876575.1"
                     /translation="MVTAMRTPRAKPRSRLAPSRRPRLADVALAAGVSAMTVVRVLRE
                     PAKVAAATRARVERVIRETGYTPDLIARGLASNRSGLIAAVIPVLTNSLIAEIMQGLT
                     DALVPKGLHLLLGASGFSVAEEEALVRAFLSRRVDGIYLSGVAHSAETVRMLQRADIP
                     VVEGGNLTTHPIDMLVGFSNVDAARAITRYLIQRRYASIGYIGAFPKDNDRARDRRNG
                     YEQAMKAGRRRIDPGLCEETTLDVDAGGRAMASLLERRPGVRAVFCSADALAVGALYE
                     CQRRGLAIPRDIAIAGFDDIPIAGQVVPALTTIRVPRYEIGQRAGAMLCDRLAGRTVR
                     RRIIDTGFELVPRESA"
     gene            7102..7566
                     /locus_tag="VFO53_09635"
     CDS             7102..7566
                     /locus_tag="VFO53_09635"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HET8876576.1"
                     /translation="MPRRLTKWTIAFFAIGLALSFAASNAEQHERMSKTAPAHHGAPA
                     MDASVPAGPLVIDSAKSSEELLAQSMAVMSRDMELVPMTGDPDHDFAAMMIPHHQGAI
                     DMAKVELLHGKNAALRRLAQEIIVTQRSEIDVLRMQLTTMRGAAPGSGHSAR"
     gene            7571..9031
                     /locus_tag="VFO53_09640"
     CDS             7571..9031
                     /locus_tag="VFO53_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876577.1"
                     /translation="MTRFGSRVIGKLAPAVAIAIAAPLCQPAIAGQAPGPASAPDMPI
                     SSRDRVYLADQTSNTVSVVDPAAQRLLGVIRLGDPLPGSLNPRYKAQLLVHGMGFSPD
                     HRTLAVVSTGSNSVAFIDTQTNLAKQILYVGRAPHEVMFTPDGSEAWVTIRGENYVQV
                     IDARSFKLTDRIEVPNGPGMTIFSPDGRYGYVCSSFSPETVVIDTHSHRLVGRIAQAS
                     PFCPNIAATPDGRQVWLTLKDVGKTQVINARPPFDTLAVLDTGPITNHVNFARNANGQ
                     FAYITIGGSNTVKVYTTGDQPSLVTTIPVGDLPHGIWPSGDGTRIYAGLENGTALAAI
                     DTSSNTVVATIPTGQSPQAIVYVPRAVPSGTGVANLVPIGTSGAAVHLSLVAAGTNDV
                     RTTVAVNDQGLIDLLEAAVTGLEPKQSYVVALAANADGSGTLEPIARFTAGVDGGAIP
                     VTIGPLRAALAGNDPAARRFLVIARADDLAVVQVQE"
     gene            9035..9928
                     /locus_tag="VFO53_09645"
     CDS             9035..9928
                     /locus_tag="VFO53_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HET8876578.1"
                     /translation="MKLTSGFAVLAIAAIVATSATGAEPAWMTGVGDALGKKGTAQPG
                     GVYRVGLPRTDLKVTLDGVEIKPALALGSWLAFANRGNRGVVMGDLVLLPGEVNPVMK
                     KLEDSGIEITALHNHLLRNEPFTLYMHVLGHGNPVKLATALHDALAESRTPLQDASGP
                     PPSTPAAIDLDTAMIDRVLGHKGMVSGGVYQVNIARAGRIRDAGMDVPQAMGSATGIN
                     FQPTGSGKAAITGDFVLTAREVNPVLKALRDNGIEVTALHNHMLDDQPRLFFMHFWAN
                     DDVGRLANGLRIALAHVNTAH"
     gene            9963..11006
                     /locus_tag="VFO53_09650"
     CDS             9963..11006
                     /locus_tag="VFO53_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET8876579.1"
                     /translation="MKHGIAIALASALLFGISTPLAKTLVGTVSPLLLAGLLYAGSGL
                     GLVLVLAVRSLAARGSFSWRLPRHEWRWLAAAVLLGGVVAPVALMFGLVSSAASTASL
                     LLNLEAVFTALIAWILFRENFDRRVVAGMLAIVAGGALLASTPGAYAGAPVGILLIVA
                     ACFCWALDNNLTRNVASSDAVVVACVKGLIAGAVNIALAFLVGVDLPQRGTLAAAALL
                     GFLGYGVSLVLFVVALRHLGTARTVAYFSVAPFFGAAVALLLQGDPITWELVVAGLVM
                     AAGVWLHLTERHEHPHAHAAQEHSHRHLHDEHHQHAHDSWNGNEPHSHRHVHAPLVHS
                     HRHYPDMHHRHRH"
     gene            11120..12712
                     /locus_tag="VFO53_09655"
     CDS             11120..12712
                     /locus_tag="VFO53_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876580.1"
                     /translation="MLHIRRESTSRSGKQSATGTDSGESIRPPFRAKVLPVVLAASLG
                     FAAAGFLAFRDIARAQPSHTVPHAQSRHAAGPKASGDLSGRKPSGDLSGKSFEAQIAA
                     NTRDLFTEGRDTFRFDTFGDEDFWGGALQLHQAIEGATFGGVGPGITPRAALDLGLKV
                     DADALQASQGRLQSGRLDLDSPAGTLALLKANAIVGLTGFFNADGSLKSVGIQCALCH
                     STVDNSVAFGIGKRLDGWANRDLDVGRVIASAPNLTPFATLLGIDVAAVRAVLLSWGP
                     GKFDAELALDGKAFNPQQVSHGVVTATNVSGATLLPNAYGLAGFNQHTWTGAWGTVTY
                     WNAFVANLEMHGKGRFFDPRLDNPAQFPIAAANGFGDLPHISPDDDRITSKLAALHFY
                     QLALPAPAPRPGIDFDEAAAGRGDELFSGKAGCNNCHVEPLWTEPGWNLHKPSEIGID
                     SFEADRAPDHTYKTMNLAGLFVRENGLFMKPANKGRYFHDGRFATLGDVVDHYDIFFK
                     LGLDAQEKHDLVEYLKSLPEKS"
     gene            complement(12749..>13166)
                     /locus_tag="VFO53_09660"
     CDS             complement(12749..>13166)
                     /locus_tag="VFO53_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876581.1"
                     /translation="ARTDMAFELLDKVGLPEVSFYKYPHEFSGGQRQRIAIARCLTMR
                     PDILICDESVSALDVSVQAQVLNLLQDLQDEFHLSYIFISHDLAVVKYISDEVMVMNE
                     GEIVEIANSDEIYRHPKMPYTRKLLASIPKGWQAGQ"
BASE COUNT         2222 a         4440 c         4322 g         2182 t
ORIGIN      
        1 gtggtgatgc aggggatggc atccgcaatg cttcatgatc gggctccgtt gatcggtaac
       61 actacacggc cggtaccggc gcaccggcgg cgacgagccc ttccgccttg agctcggccc
      121 agaaatcacg gggaatgtct gcctggcaat gtgcgacatt cgcgtcgact tcgcgccggc
      181 tgcgcattcc cgtcacgacg ctcgccaccg ccgggtgcgc gagcggaaac tgcagcgccg
      241 ccgccgcaat ggcaacgcga tggcgcgcgc agatcgcctc gatccttttc gtgcgcgaca
      301 ggacttccgc gtcggcttcg gcatagaagt acgtcgcacc ggtcgtggcg ccggtggcga
      361 gaatgcccga gttgaacggc gcggccatca gcaccgaaat gttccgacgt tcgcactcgg
      421 gcatgaacgt gtcgagcgac gtgtgatcga gcagcgtata gcggcccgcg agcatgaaac
      481 agtcgaagtc gccgtcggcg gcgaagcgca gcaggatctg ccagtcgttg acgcccacgc
      541 cgaacgcctt gatcgtgccc gcggtccgca ggtcgcaaag cgcacggtgc gcgccttcca
      601 tcgcctcgcg ataacgttgc tcgacgagat cgccctgcca gcgccggttc acgtcgtgga
      661 tcagcacgat gtcgagcgcg ttcgtcccaa ggcgctgcat gctcgcctcg agcgagcgca
      721 gcgttccgtc gtagctgtag tcgaactcga cgttgaatgg cgagctgccg gcgcagatac
      781 ccggcgcggg cccaccctcg agcggacgca gcagcctgcc gactttcgtc gacagcacga
      841 ccgagcgccg atcgatgccg cgaagacacg ctcccagccg atgctcggac aagccaaggc
      901 cgtagagcgg cgcggtgtcg aaatagcgca ggccggcggc gtacgcggcg gcgaatgcgc
      961 cgtcgatcga tgcatcggtg tcgtcattgg cgaagctgcc gaacgggagc gcgccgacgc
     1021 cgagttgcgc gacggcaaga tcgcgccgcc cgatccttcg cgccgcggcg ggatcgaatc
     1081 tccccatgcg gcacgtcacg cgggcggcag ccgcacctcg acgcccgcgg cgtcctcgcg
     1141 cagcttcagc aacgcgccga catcgtcgtc gtgcaagccg cgctgcatcg cctcgtcgag
     1201 actcgcgagc gccgcgcgcc ccacgggcgt aatcacgccg agcctgcgcg ccatctcgag
     1261 cgcgagccgg caatccttgt gcgcgagctt cagcatgaat cccgggctga agtcgcccgc
     1321 gagcggccgc ttcggcaacg cgatggcaag ggcgttgttc cacgccatcg tcgtgcgcag
     1381 aacgctcatc atcgtgtcga gcgtcagccc cgatttggct ccggtcacga gcgcctcgat
     1441 cgacgcctcg gaaacggcgg tggcgagcag gttgttgatg agcttcatcg tctgcccggc
     1501 gccaaggcct ccgcaatgga agaagtcggt gcccatgcaa tcgaggatcg gccggcagcg
     1561 cgcgacgacg tcgtcggggc cgcccaccat cagcgtgagc gtgccggcga ccgcgtggtc
     1621 ggcggtcttg ccgacagggc tgtcgatcat cgacgcgccc ttcgacgcga tctgctcgcc
     1681 gacgttacgc gtggtatcgg gatcgatggt gctcatgtcg atgtagaccg agccggcgcg
     1741 gatcccggcg acgatgccgt cggccccgag tgccacccgc tcgacatccg gcgcatcggg
     1801 aagcatcgtg atgacgaaat cgctcgcggc cgccacctcc ttcggcgtgc gcgcggcgag
     1861 cgcaccggcc gcggtgagcg aggccaccgc ggccggcacc acgtcggcga cgaccagccg
     1921 gtggccctta tggagaaggt tgcgcgccat cggcgcgccc atcgtgccga gtccgatgaa
     1981 acctacggta atcatgattc ccccacgctc gcggcttcgc cgcttcgctg ccccccgagg
     2041 gggcgcatcg cacggctcgg ggcggccctt cgccgcgcgc tcacgtccgc gtaccccagc
     2101 tcggcatatc ccacactggc gcgccgcgga ggtccttcgc gttcttgtcg tggaagcccg
     2161 ccgcgtcagg tacccgtccg gtcttttcgt aggcggcctt gtcgagcggt gccgccagcg
     2221 cgaaggcgcc cttcacctga tcccacgtga gcggcttggt gatgagcttg ccgtcgacca
     2281 tgctcttcga cgtctcgagc aacgcaaccg catcgccttc ggtcggccag caccagccgc
     2341 gcttgaacag cacgtcgtcc tcgaccacct ttgcgccgag ctcgggcggc agctcccagt
     2401 atttcttcgc gagctcggac acggtgcccg ggtccatctt cgtgagcttc gccaccgcgt
     2461 cctgctgtgc ggtcacgaac gccgtcgcca cggcgggatc gctctcgatg atcttgcggc
     2521 tgccgatcca gaacgagcgg tgcaggtaga agccgtcggg atagaacggc gatttcttca
     2581 ccgaaggcag cagaatgccg gcgccctcgc cagccggccc cttgtagtgg ctttcggtgt
     2641 acccgaacga gttgatgatg ccgacgatgc cgagctcggc gctcgccttg agaaatgccg
     2701 ggtagatcac gacggccgca tccatgccgc ccgggatcgt cgccgcctgc gcctgcgtcg
     2761 gcgtgttgac caccgtgatg ttgaatgcct tcgggtcggg attgccgagc tcgtagcgca
     2821 gcatctgcga cagcacgttg tacggatcgc cgccgaggag cgcccccacc gtcttgccct
     2881 tgagatcggc gacgttgcgg atcggcgaat ccttgcgcgt cgcgagcacg aagcggaaat
     2941 ggccttcgcc caccgtcatg atctggatcg gctggttctg cgcgacgagg cgcacgatcg
     3001 gcgtgttgcc ccacatgccg aagtcgaggt tgccggagac gaacgcttcg acctgcggcg
     3061 ccgcggacgg atagtcgcgg tagtcgacgc tgaggtcgta gcccgcatcg ccggcgaagc
     3121 gctcgaacgt cttgttgtcc atcatgtact tcgtgaccgg cgaattgccg gcggcccacg
     3181 gctggcggcc gaccgacacc ttgagcgcct tcctgccctg cgcgcggacg atcgccggtg
     3241 cggcgaatgc gagtgacgcg gcgccggccg tggcgatgaa acggcgccgc gacacgctct
     3301 tgcgatcctt ctcgatgctc atgtctgtct tcctccttcg tgggtcgatc aggtgacggt
     3361 cgcgcgatac gcttcgtcac gaataaggct ccagatgtgc tggctgagag cctggaactc
     3421 ggccgacgcg cgcgttgcat cgttgcgcgg acgcggcagc ggaacgtcga tcaacgcctt
     3481 gaggcgtccc gggcgcgtcg tcatgacggc gacgcggtcg gcgagaaaca cggcctcgtc
     3541 gatcgcgtgc gtgatgaaca cgacggtgcg ccgtcgagcg gccggtcggt cctgccccca
     3601 gatgcgcagc agctcgtcct gcaggatgag ccgcgtctgc gcgtcgaggg cggcgagcgg
     3661 ctcgtccatc agcagcactt ccggctcgtt cgccatcagc cgcgcgaggg ccgcgcgctg
     3721 gcgcatgccc ccggatagct ggtgcggata cttgtcttcg gcgccgttga gcccgacgag
     3781 gtcgatgcaa tcctgcacgc ggcgcgcacg cgcatcggtg gaaaggccgt tgcccgaggg
     3841 accgtatttg aggccgaagc cgacattgcc ttgcaccgtc tgccagggga agagcgcgta
     3901 gtcctggaac atcacgccgc gcgcgggccc cggaccggtg atctcgcgat cgtcgagcgt
     3961 cacgctgccg gcactcggtc gctcgagccc ggccaggata ttgagcaccg tcgtcttgcc
     4021 gcagcccgat gggccgacga tcacgaggaa ctcgccctgc acgatgtcga gcgtgaaatc
     4081 gtcgacggcg atcgtcggtt tcgccccgcc cacgtcgaag tgcttggcaa cgtgcgcgag
     4141 gcgcagcttc gcgcccgcga tgggcgtggc gccggtcatc gcggcgtcca gggcatcaaa
     4201 agccgcatgc cgatgcgcag gagccggtcg agcacgagtg cgacgaggcc caccgcgatc
     4261 atgcatacga tgatgcggtt gagctcgatg ctgtacgcat agaacacgaa catcatctgg
     4321 ccgatccccc ccgatccgcc gccgccggcc ttcgcgccga cggcgagctc ggcggcgacg
     4381 atcgccgtcc aggcaacgcc cattgcaatg cgcaggccga cgccgatggc cggcagcgcc
     4441 gccggcacga tcaccgtgcg gatcgtgcgc aaccggggca cgcccatcgt cgccgcggcg
     4501 cgcagcaacg ttgcgttgac tcccttcgca ccggcgacgg tattgatgac gatcggaaag
     4561 aacgccgcgt acgcgacgat catcacggcg gcgccggtcc cggtgccaag ccacaggatt
     4621 gcgagcggca ggatggcgat cgccgcgatc ggccggaagc tctccaccag cggatcgacg
     4681 ttgcgctcga cgccgcggaa cgtgcccatc gacagcccga gcggaatggc gatcaccgcg
     4741 gcgacggcaa agcctgcgaa tacgcgcgac aggctctgca tgatcgcgag cggcaactcg
     4801 cccgagcgca gcaggtcgac cgccgccgtg accacgtgcg tcggcaccgg catgcgcggg
     4861 ttgtgcgaag cgctgcccca tgcctgccac agcgcgagca gcagcagcgg cagcccgagg
     4921 ccgcggacaa tgcgcgcggc gatcatcgca ccgcctcgcg cgcccacggc gtgaaccagc
     4981 ggccgagcgc gcgcacgccg aggtcgcaca ggtagccgag cacgccgatc atcgcgatga
     5041 acgacatcac ctgcggcgtt gcgagcagct cgcgatacca ctcgacggca tagccgagcc
     5101 cgctgggcgc gccgatcagc tcggcggcaa cgaccgcggt ccacgacgat gcgacgccga
     5161 gccgcagcgc gaccatcagc gacggcagcg ccgccggcac gacgacgcgg gtgacgatcg
     5221 tgcgccgcgg cacgcccatc gtgcgcgccg cgtcgatgag ccgccggtcg acgctgcgca
     5281 cgccggctat cgtgccgagg aggatcggaa agaagccggc gtacgtcatg atgaagaccg
     5341 gcagcgcgtt gccgatgccg aagatgaaga gtgcgagcgg aatccacgcg atgccggaga
     5401 tcggccgcag gatttcgatg atcgggtcga aaatctggtc gagcgcgcgt gaaagcccca
     5461 tcgcgaggcc gagcgggatc gcgaggagcg aaccgagggc aaggctcacc gccatgcggc
     5521 cgaggctgat gcccgcatgc aggaacagct cgaggtcgcg cgcgagatcg agcatggcgc
     5581 cggccacggt aagcggcgac ggcagcagga tggcgttcgc cgtcagcatc gcgacgagcg
     5641 tccacacgac gaagaacgcg agcatcgtgc gcgcgtagat caacgccggc cggtacccag
     5701 cgaacgcgtt gcgcacgccg ttcgacacct ccgtcccccc aaccggcgct cgcgccgtgt
     5761 tatgttatcg cttacatgtt agcgcttcca ttttgccgct gtcaaaccac gccggatggc
     5821 aggcgaccgg cggctccatg gtgacggcaa tgcgcacgcc gcgtgcaaaa ccgcgctcgc
     5881 gcctcgcgcc ttcgcggcgt ccgcgactcg ccgacgtcgc gctggccgcc ggcgtgtcgg
     5941 cgatgacggt ggtgcgcgtg ttgcgggagc cggcgaaggt ggcggccgcg acgcgcgcgc
     6001 gtgtcgagcg cgtgatccgc gaaaccggct acacgcccga cctgatcgcc cgcggcctcg
     6061 cgtcgaatcg ctcgggcctg atcgccgcgg tgatcccggt gctgacgaac tcgctgatcg
     6121 ccgagatcat gcaggggctg accgacgcgc tggtgccgaa gggcttgcac ctgctgcttg
     6181 gcgccagcgg attttccgtc gccgaagagg aggcgctggt gcgcgcgttc ctgtcgcggc
     6241 gcgtcgacgg catctacctg agcggcgtcg cgcacagcgc cgagacggtg cgcatgctgc
     6301 agcgggcgga cattcccgtc gtcgaaggcg gcaacctgac cacgcatccg atcgacatgc
     6361 tggtgggctt ctcgaacgtc gacgcggcac gcgcgatcac gcgctacctc attcagcggc
     6421 gctatgcatc gatcggctac atcggcgcgt tcccgaagga caacgatcgc gcgcgcgacc
     6481 ggcgcaacgg ctacgagcag gcgatgaagg ccgggcggcg caggatcgat ccgggcctgt
     6541 gcgaggaaac gacgctcgac gtcgacgcgg gcggacgcgc gatggcgtcg ctcctcgagc
     6601 ggcgtcccgg cgtgcgcgcc gtgttctgct cggccgacgc gctcgccgtc ggcgcactct
     6661 acgaatgcca gcggcgcggg ctcgcgatac cgcgcgacat tgccatcgcg ggcttcgacg
     6721 acataccgat cgcggggcag gtggttcccg cgttgacgac gatacgggtt ccgcgctacg
     6781 aaatcggcca gcgtgccggc gcgatgctgt gcgatcgcct ggccgggcgc acggtgcgca
     6841 ggcgcatcat cgacacggga ttcgagctcg tgccgcgcga aagcgcatga gcttgcgcgc
     6901 gcagcgttcc cgcgacgcgg attaatccgc aatctgcgtc tgtttgccgt agattgtggc
     6961 ggttactgcc cggctgcttg ctgccgtttc gcgatgctca tttcacgccc gccgaagatt
     7021 gcatcgaacc gcgcgaatcc atgaactatg cctttgcgtc gcgctcttat gcggtgcgac
     7081 gaacgttccg tattcagggc catgccacgc cgcttgacca aatggacgat cgcctttttc
     7141 gccatcggcc ttgcgctgtc gttcgcagca agcaacgccg aacagcacga gcggatgtcg
     7201 aagacggcac cagcgcacca tggcgcgccg gcgatggacg cgtccgttcc cgccggtcca
     7261 ttggtgatcg attcggcaaa atcgagcgag gaattgctgg ctcaatcgat ggccgtcatg
     7321 tcgcgcgaca tggagctggt gccgatgacc ggcgatcccg accacgactt cgccgcgatg
     7381 atgattccac atcatcaggg agcgatcgat atggcgaagg tcgaactcct gcatgggaag
     7441 aatgccgcac tgcggcgcct ggcgcaggaa atcatcgtta cccagcgctc cgagatcgac
     7501 gtgctgcgca tgcagctcac cacgatgcgc ggtgcagcgc cagggtccgg acacagtgcg
     7561 aggtagaaac atgacgcgct tcggaagccg cgttatcggg aagcttgcgc cggctgttgc
     7621 gatcgccatt gcagcgccgc tgtgccaacc ggcgatcgcc ggccaggcgc caggcccggc
     7681 aagcgcgccg gacatgccga tcagctcgcg ggatcgcgtt tacctcgccg accagacgtc
     7741 caataccgtg tcggtcgtcg atccggcggc gcaacggttg ctcggcgtca ttcggctcgg
     7801 cgatccgctg cccggcagcc tgaacccgcg gtacaaggcg caactgctcg tgcatgggat
     7861 gggcttctca cccgaccatc gcacgctcgc cgtggtgtcg accggctcca attcggtcgc
     7921 cttcatcgac acgcaaacga atctcgccaa gcagattctc tacgtcgggc gggctccgca
     7981 cgaggtgatg ttcacacccg acggcagcga ggcttgggtc acgattcgcg gcgagaatta
     8041 cgtgcaggtg atcgacgcgc gatcgttcaa gctgaccgat cgcatcgagg tccccaacgg
     8101 cccgggcatg acgatcttct cgcccgacgg ccgctacggc tacgtgtgct cgagcttcag
     8161 cccggaaacg gtcgtcatcg acacgcacag tcatcgcctc gtcggccgga tcgcgcaggc
     8221 gtcgccattc tgtccgaaca tcgccgcgac gccggacggc aggcaggtgt ggttgacgct
     8281 gaaggatgtc ggcaagacgc aggtgatcaa cgcgaggccg ccgttcgata cgctggccgt
     8341 gctcgatacc ggtccgatca ccaatcatgt caactttgcc cgcaatgcca acggccagtt
     8401 cgcctacatc accatcggcg gatcgaatac ggtgaaggtc tacacgaccg gcgatcagcc
     8461 gagtctcgtc acaacgattc ccgtcggcga ccttccgcac ggcatctggc cgtcgggtga
     8521 cggaacccgc atttatgccg gactcgaaaa cggaacggcg cttgccgcga tcgacacatc
     8581 gtcgaatacc gtggtcgcca ccatccctac cggccagtct ccgcaggcga tcgtttatgt
     8641 gccgcgggcc gtgccctcgg gtaccggtgt ggccaacctc gtaccgatcg gcacctcggg
     8701 cgcggccgtc catctgtcgc tggtcgccgc cgggacgaac gacgtcagga cgacggtggc
     8761 ggtgaacgat caggggttga tcgaccttct cgaagccgcg gtcaccggac tcgaaccgaa
     8821 acaatcgtac gtggtggcgc tcgcggcgaa tgccgacggt tccgggacac tcgaaccgat
     8881 cgcccggttc actgcgggcg tcgatggcgg cgcaattccc gtgacaatcg ggccgttgcg
     8941 cgccgcactc gcaggtaacg accccgcggc acgccgcttt ctcgtcatcg cgcgtgccga
     9001 cgatctcgca gtcgttcaag tccaggagtg accgatgaag ctgacatccg gatttgcggt
     9061 actcgcgatc gccgcgattg tggcgacgtc cgccactggc gccgagcccg catggatgac
     9121 cggcgtcggt gatgcactgg gcaagaaagg aacggcgcag cccggaggcg tgtatcgggt
     9181 cggtcttccg cgcaccgacc tgaaggtgac gctcgacggc gtggagatca agcccgcgtt
     9241 ggcacttggt tcatggctcg ccttcgccaa tcgcggtaat cgaggcgtcg tgatgggtga
     9301 ccttgtgctc ttgcccggcg aagtcaatcc ggtgatgaag aagctcgagg acagcggaat
     9361 cgagatcacc gcgctgcata accacctgct gcgcaacgaa ccgttcacgc tgtacatgca
     9421 tgtgcttgga cacggcaatc cggtcaagct cgcgaccgcg ctccacgatg cgctcgctga
     9481 aagcaggacg ccgctccagg atgcatccgg tccgccgccc tcgaccccgg ctgcgatcga
     9541 cctcgacacc gcgatgatcg atcgcgtgct cgggcacaag ggcatggtca gcggcggcgt
     9601 ctaccaagtc aatatcgcgc gtgccggacg tatcagggat gccggcatgg acgttccgca
     9661 agcgatggga tcggcgacgg gcatcaactt tcaacctacc ggcagcggca aggcggccat
     9721 taccggcgat ttcgtgctta cggccaggga ggtcaacccc gtgctgaagg cgctgcgcga
     9781 caacggcatc gaagtgaccg cgttgcataa ccacatgctc gacgatcaac cgcgcctgtt
     9841 cttcatgcac ttctgggcga acgacgacgt cgggaggctc gcgaacggac tcaggatcgc
     9901 gctggctcat gtgaatacgg cgcattgagc ttcgctcgcg ctcgccgtcc accatcgacg
     9961 cgatgaagca cggaatcgcc atcgcgctgg cgtcggccct gctcttcggg atcagcacgc
    10021 cgctcgcgaa aacgctggtc ggaacggtat cgccgttgct gctcgccgga ttgctttacg
    10081 cgggatcggg cctcgggctg gtcctcgtgt tggcggttcg cagcctcgcc gcgcgcggca
    10141 gcttttcatg gcgtctccca cgccacgaat ggcgctggct cgcggcggct gtcctgcttg
    10201 gcggtgtcgt tgcgccggtc gccttgatgt tcggcctcgt gtcgagcgct gcctcgaccg
    10261 catcgctgct gctcaatctg gaagccgtgt tcacggcgct gatcgcctgg atcctctttc
    10321 gggaaaactt cgatcgccgc gtcgtcgccg gcatgctcgc catcgtggcc ggcggcgcgt
    10381 tgctcgcgtc gactcccggc gcgtacgccg gcgcgccggt gggcatcctg ttgattgtgg
    10441 ctgcatgctt ctgctgggcg ctcgacaata atctgactcg aaacgtcgcg tccagcgacg
    10501 ccgtcgtggt tgcctgcgtg aagggactga tcgcgggcgc cgtcaacatc gcgctcgcat
    10561 tcctggtcgg tgtcgatctg ccccagcgcg gcacgctggc ggcggctgcg ctactgggat
    10621 ttctcggcta tggcgtaagc ctcgtgctgt tcgtcgttgc gcttcgacac ctcggcaccg
    10681 cgcgcacggt cgcctacttt tcggtggccc cgttcttcgg cgccgcggtc gcgttgctgc
    10741 tgcagggcga tccgatcaca tgggagctgg tcgttgcggg cctggtgatg gcggccggcg
    10801 tctggttgca cttgaccgaa cgccatgaac atccgcacgc ccacgcagcg caggagcaca
    10861 gccaccggca cctccatgac gaacaccatc agcacgcgca cgattcctgg aacggaaacg
    10921 agccgcacag ccatcgccac gtgcatgcgc cgctcgtcca ttcacaccgg cactatcccg
    10981 acatgcatca tcggcatcgc cattagggcg caccgggcgg gcgatgctcg atgcatcgcc
    11041 atcgggcgat agcgatcctg cggaatagcg gcgacgacgt cggtgttgac gatggcgcga
    11101 cagctcgaag gaggacgcca tgttgcacat ccgacgcgag tcgaccagcc gatccggcaa
    11161 gcaatcggca acgggcaccg attccggaga atcgatacgg ccgccattcc gggcgaaggt
    11221 tttgccggta gtcctcgcgg catcgctcgg cttcgccgcc gccggattcc tcgccttcag
    11281 ggatatcgcg cgtgcccagc cgtcgcatac ggtgccccac gcgcaatcac gccatgctgc
    11341 gggcccgaaa gcgtccggcg atctctcggg ccggaaaccg tccggcgatc tttccggcaa
    11401 gagcttcgag gcgcagatcg cagccaacac gcgcgacctg ttcaccgaag gccgcgacac
    11461 cttccgcttc gacacgttcg gcgacgagga tttctggggc ggcgcgctgc aactgcacca
    11521 ggcaatcgaa ggcgccacgt ttggcggcgt cggtccgggc atcacgccgc gcgcggcgct
    11581 ggatctcggc ctcaaggtcg atgccgatgc gcttcaggcg agccagggcc ggttgcagag
    11641 cggccgcctc gatctcgaca gtccggccgg cacgctggcg ctgttgaagg cgaatgcgat
    11701 cgtcggcctc accggattct tcaacgccga cggttcgctg aagtcggtcg gcatccagtg
    11761 cgcgctgtgt cattcgaccg tcgacaactc ggtcgctttc ggcatcggca agcgtctcga
    11821 cggctgggcg aaccgggatc tggacgtcgg cagggtcatc gcctcggcgc ccaatctcac
    11881 gccgttcgcg acgctgctcg gcatcgacgt cgccgccgtc agggcggtat tgctgagttg
    11941 gggtcccggc aagttcgacg ccgaactggc gctcgatgga aaggcgttca atccgcaaca
    12001 ggtgtcgcac ggcgtcgtca cagcgacgaa cgtgtcggga gcaaccctgc ttccgaacgc
    12061 gtatgggctt gccggattca accagcacac gtggaccggt gcatggggaa ccgtgaccta
    12121 ctggaacgca ttcgtcgcca acctcgaaat gcacggcaag ggtcgcttct tcgatccgcg
    12181 actcgacaat ccggcgcaat tcccgatcgc cgccgcgaac ggattcggcg acctgccgca
    12241 catcagcccc gacgacgacc ggatcacgtc gaagctcgcg gcgctgcact tctatcagct
    12301 cgcgttgccc gcgcctgcgc cgagacccgg aatcgacttc gacgaagcgg cagccgggcg
    12361 cggcgacgag cttttcagcg gcaaggcggg atgcaacaac tgccacgtcg agccgctatg
    12421 gaccgaaccc ggctggaatc tgcacaagcc atcagagatc ggcatcgaca gcttcgaggc
    12481 cgatcgcgcg ccggatcata cgtacaagac gatgaacctc gccgggctct tcgtgcgcga
    12541 aaacggtctc ttcatgaagc cggcgaacaa gggccgctac ttccacgacg gacgattcgc
    12601 gacactcggc gacgtggtcg atcactacga catcttcttc aagctgggcc tcgacgcgca
    12661 ggagaagcac gacctcgtcg aatatctgaa gtcgcttccg gagaagtcgt gacgcccgcc
    12721 ttttgaaccg tcggcggcgt gccgcgatct actgccctgc ctgccagccc ttcggaatcg
    12781 acgccagcag cttgcgcgtg tacggcatct tcggatgccg gtagatctcg tcggagttgg
    12841 cgatctcgac gatctcgccc tcgttcatca ccatcacttc gtcggagatg tacttgacga
    12901 ccgcgaggtc gtgcgagatg aagatgtacg acaggtggaa ctcgtcctgc aggtcctgca
    12961 gcaggttcag cacctgcgcc tgaaccgaca cgtcgagcgc cgacaccgat tcgtcgcaga
    13021 tcagaatgtc gggacgcatc gtgaggcagc gcgcgatggc gatccgctgc cgctgcccgc
    13081 ccgagaattc atgcggatac ttgtagaacg acacctcggg caatccgacc ttgtcgagca
    13141 gctcgaacgc catgtcggtg cgcgcc
//
LOCUS       DASSVX010000223         8854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_975920, whole genome shotgun sequence.
ACCESSION   DASSVX010000223 DASSVX010000000
VERSION     DASSVX010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8854
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975920"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /gene="accD"
                     /locus_tag="VFO53_09665"
     CDS             <1..657
                     /gene="accD"
                     /locus_tag="VFO53_09665"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET8876582.1"
                     /translation="EGRFEIGLEVVPVDTLKFKDTKKYSERLEAALEDTGETDALVVM
                     QGSVKSVPLVLAAFEFNFMGGSMGSVVGERFVRGVRVAYENRLPFVCVSASGGARMQE
                     GVNSLFQMAKTTAVLQELTRAKLPFVSILTDPTMGGVSASFAFVADLVLAEPGALIGF
                     AGPRVIEQTVREKLPEGFQRAEFLVEKGALDMIVDRRNMKDELARLLAMLTRQPAPSA
                     "
     gene            697..2013
                     /gene="folC"
                     /locus_tag="VFO53_09670"
     CDS             697..2013
                     /gene="folC"
                     /locus_tag="VFO53_09670"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET8876583.1"
                     /translation="MGPVTAVSADSHPRTLAQWLAFIETLHPKSIALGLERIAAVAAR
                     MAMRITCPVITVAGTNGKGSTCAIAESIYRCAGYRTGLYASPHLLRFNERVRIAGKEA
                     EDAALADAFAVVEAARDLGDGASTPLTYFEYTTLAALHLFAAARLDVLILEVGLGGRL
                     DAVNLVDADVAVVTTIGIDHVDWLGATREAIGREKAGIFRKGRTAICGDSEPPHSLVD
                     AAHAAGATLWRIGVDYRYAAQRMQWRYDGPGGARYGLPLPALRGAYQLANAATALAAL
                     DALRVRLPVAAGAVRDGLVQVELPGRFQVLPGRPAVVLDVAHNPQAAGVLSETLSTMG
                     FFPRTLAVFGMLGDKDIDAVVRALAPRVDCWYIAPLPGPRGALAAQLATALTRAGVDA
                     GAILAFDDIEQAFDAARSAAGETDRIAAFGSFLTVAAALAAAQRKR"
     gene            2031..2831
                     /locus_tag="VFO53_09675"
     CDS             2031..2831
                     /locus_tag="VFO53_09675"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET8876584.1"
                     /translation="MAEPADVNVDELRRRARRRLVGAVVLALGVAVVVPMLLETEQKP
                     LGDDVAVRIPPVDSGKFVNRLNDGTTKGDPNAKAADDATKPAPATSAATEAAPERQQK
                     YDTRVPGGASASSATMPSDAATGTPEPGTTSPPNTAPVIITDKSTPAPAQAPAQKAAA
                     SESPKATAAASHRAPEPARSAHDEGGASQHDAFAVQLAAFADDRGANSLAGRLKKSGY
                     AAYTEPLKTSKGTLWRVRVGPYPSRDAALAARDKLKTEGQSGIVAAIK"
     gene            2879..3415
                     /locus_tag="VFO53_09680"
     CDS             2879..3415
                     /locus_tag="VFO53_09680"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET8876585.1"
                     /translation="MTTFDWIVVCIVGLSTLFAFFRGVIREVVALVAWVAGLVCAILF
                     TPILGAMLPDMFGHPAIRYVLAFTLILVAALLLGALVAWPLSTVVRAAGLGFVDRFLG
                     SIFGFARGVLLMIAFVFVAGLTPLPRMLWWQSAALVPPLVAGVYALRPHLPAQIAGRL
                     DYSPHRIAPKVAPAEQKA"
     gene            3521..5026
                     /gene="purF"
                     /locus_tag="VFO53_09685"
     CDS             3521..5026
                     /gene="purF"
                     /locus_tag="VFO53_09685"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET8876586.1"
                     /translation="MCGILGAVAHTPVNQLLYDGLLLLQHRGQDAAGIVTSEGPVFHT
                     YKGPGYVRDVFRTRNMRELSGTTGIAHCRYPTAGSAFSDLESQPFYVNSPFGLTLGHN
                     GNLTNSEALKRELWELDFRHVNTNSDSEVLLNVLALELERAAQHHRLDPEAIFRAVAG
                     VHRRCRGAYAVVAMIAGYGMLAFRDPFGIRPLIIGSNQAMGGPEYLVASESVALEPLG
                     FSVLRDVEPGEAIFVDQAGTFHARQCAAHPSLNPCIFEYVYLARPDSVMDGTSVYEAR
                     LRMGAALATKIRDIAQAQDIDVVIPIPDSSRPYALELANRLGKTYREGFVKNRYVGRT
                     FIMPGQALRRKSVRQKLNPIGMEFKDKVVLLVDDSIVRGTTSREIVQMARDAGARKVY
                     FASASPPVRFPNVYGIDMPNQDELVATGRTEAEIAREIGADLLVYQDLEALKEAVRFG
                     NPRLTDFEASCFDGRYITGDITSDYLRELALERDQSRGEADADVAEDASAG"
     gene            5281..6522
                     /locus_tag="VFO53_09690"
     CDS             5281..6522
                     /locus_tag="VFO53_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HET8876587.1"
                     /translation="MTENHHERGFTTTILHSDRDAAIEHGALHKPLHVSVAYGYKDAA
                     DLVAVFQGRQSGYAYGRQGNPTTAALEAKVTAMEKGVATVCFSTGMAAIEATLVALLR
                     GGDHIVASSFVFGNTNSLLNTLGDHGTPVTFVDATDVANVARAVTPATRLVFVETIAN
                     PRTQVADLARIGELCRERGLIYIVDNTMTSPWLFRPIDVHAGLVINALTKYIGGHGNA
                     LGGAVTDTGLYDWTRFPNIAEPYKTQKPPLWGITQIRKKGLRDGGATLSPEQAHHLAV
                     GAETLALRMDRQCANAQALAEFLASHPKVEAVYFPGLRSHPEHERSRSLFRAPGALLS
                     FDLAQGEDVLACLNRLRVVVLSSNLGDNRTLAIPVAQTIFWEMGPARRTEMGIADSLV
                     RVSVGIEDRDDLIDDFRRALR"
     gene            complement(7022..7573)
                     /locus_tag="VFO53_09695"
                     /pseudo
     CDS             complement(7022..7573)
                     /locus_tag="VFO53_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
     gene            complement(7570..8298)
                     /locus_tag="VFO53_09700"
     CDS             complement(7570..8298)
                     /locus_tag="VFO53_09700"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HET8876588.1"
                     /translation="MKPTLLLSDLHLAPDRPAAAAAFEAFARGPARAAAAVYVMGDLF
                     DSWIGDDQCREPFARSIVAALRGISDAGVALYVARGNRDFLLGDAFARDTGATLLGEQ
                     TVVDLGGTPTLVTHGDELCTDDDAYQRYRSFTRDPAMQRRLRRLPYRVRRWIAAWLRR
                     GSRAATARKPESILDVNAAAVEHAFRMHAVTRMIHGHTHRPARHSTRVDGQPRERIVL
                     ADWDEHGHYIAIDADGVHTRHIVG"
     gene            complement(8351..8851)
                     /locus_tag="VFO53_09705"
     CDS             complement(8351..8851)
                     /locus_tag="VFO53_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011206720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET8876589.1"
                     /translation="MTQVVLHTSEGDIALALDEENSPATVANFLQYVRDGHYDNTLFH
                     RVIPGFMIQGGGFAPGMQQRATRAPVANEAANGVRNVRYSVAMARTSDPHSASAQFFI
                     NLADNAFLDYKGPSPQGYGYCVFGKVVRGSDVVDRIAGVATGRSGFHENVPTQDVVIT
                     RAEVAG"
BASE COUNT         1450 a         2996 c         2986 g         1422 t
ORIGIN      
        1 gagggacgct tcgagatcgg cctcgaggtg gttccggtcg acacgctgaa gttcaaggat
       61 acgaagaagt attccgagcg cctcgaggcc gcgctcgagg acaccggcga gaccgatgcg
      121 ctggtcgtga tgcagggaag cgtcaagagc gtgccgctcg tgctcgcggc gttcgagttc
      181 aacttcatgg gcggctcgat gggctcggtc gtcggcgagc gcttcgtgcg cggcgtgcgc
      241 gtcgcctacg agaaccggct gccgttcgtc tgcgtgtcgg cgtccggcgg cgcgcggatg
      301 caggagggcg tcaactcgct gttccagatg gcgaagacca ccgcggtgct gcaggaattg
      361 acgcgcgcga agctgccgtt cgtgtcgatc ctcaccgacc cgacaatggg tggcgtctcc
      421 gcgtcgttcg cgttcgtcgc cgacctcgtg ctcgccgagc cgggcgcatt gatcgggttt
      481 gccggaccgc gcgtcatcga gcagacggtg cgcgagaaat tgcccgaagg cttccagcgc
      541 gccgaattcc tggtcgagaa gggcgcgctc gacatgatcg tcgaccggcg aaacatgaag
      601 gacgagctcg cgcggctgct cgcgatgctc acccgccagc cggcgccgtc ggcctgacgc
      661 caacggcgcg ggcacccgtc cgcgcgaacc gatcgtatgg gtccagtaac agcggtgagc
      721 gccgacagcc acccgcgaac gctcgcgcaa tggcttgcgt tcatcgaaac gctgcatccg
      781 aagtcgatcg cgctcggcct cgagcggatc gcggcggtcg cggcgcggat ggcaatgcgc
      841 atcacctgcc cggtgattac cgtcgcggga accaatggca agggctcgac gtgcgcgatc
      901 gccgaatcga tctatcgctg cgcgggctat cgcaccggcc tgtacgcgtc gccgcatctg
      961 ttgcgcttca acgagcgcgt tcgcattgcg ggaaaagagg cggaagacgc cgcactggcc
     1021 gacgcctttg ccgtcgtcga ggcggcgcgt gacctcggcg atggcgcatc cacgccgctc
     1081 acgtatttcg aatacacgac gctcgccgcg ttgcacctct tcgcagccgc gcgactcgac
     1141 gtgctgatcc tcgaggtcgg gcttggcgga cgtctggatg cggtgaatct cgtcgatgcc
     1201 gacgttgccg tcgtcacgac gatcggcatc gaccacgtcg actggctcgg cgcgacgcgc
     1261 gaagcgatcg ggcgcgagaa ggccggcatc ttccgcaaag gcaggacggc gatctgcggc
     1321 gattccgagc cgccgcactc gctcgtcgat gccgcgcatg cagccggcgc gacgctgtgg
     1381 cgcatcggcg tcgactaccg ctacgcggcg cagcgcatgc agtggcgtta cgacggaccc
     1441 gggggcgcgc gctacgggct tccgttgccg gcacttcgcg gcgcgtatca gctcgccaac
     1501 gcagcgacag cactcgcggc gctcgacgcg ctgcgcgttc gcctgccggt tgcggcgggc
     1561 gcggtgcgcg acggactcgt gcaggtcgag cttcccgggc gcttccaggt gttgccggga
     1621 cgaccggcgg tggtgctcga cgttgcgcac aacccgcagg cggccggcgt gctgagcgag
     1681 acgctttcga cgatgggctt cttcccgcgc acgctcgccg tcttcggcat gctcggcgac
     1741 aaggacatcg acgccgtcgt gcgcgccctc gcgccgcgtg tcgattgctg gtacatcgca
     1801 ccgcttcccg gtccgcgcgg cgcgttggcg gcacaactcg caaccgcgct cacgcgcgcc
     1861 ggcgtcgacg ccggcgccat tctcgccttc gacgacatcg agcaagcgtt cgacgcagcg
     1921 cgcagcgcgg cgggagagac tgatagaatc gcggcctttg gttcgtttct caccgttgcc
     1981 gcggcgctcg ccgccgcgca gcgaaaacgg tgaagcgcac cggcatagcg atggcagaac
     2041 cggcggatgt caacgtagac gaattgcgca ggcgcgcgcg ccggcgcctc gtaggggccg
     2101 tcgtgcttgc gctcggtgtc gccgtcgtcg tgccgatgct gctcgagacc gagcagaagc
     2161 cgctcggcga cgacgtcgcg gtcaggattc cccccgtcga cagcggcaag ttcgtgaatc
     2221 gcctcaacga cggcacgacc aagggcgacc cgaacgcgaa ggccgcagac gacgcgacga
     2281 agcccgcgcc agcgacttct gcggcaaccg aagccgcgcc agagcggcag cagaaatacg
     2341 acacgcgcgt gcccggcggc gcgtcggcgt catcggcgac gatgccttcg gatgcggcga
     2401 ccggcacgcc tgagccgggc acgacgtcac ccccgaacac cgcgccggtg atcattacgg
     2461 ataaatcgac cccggcaccg gcgcaggcac ctgcgcagaa ggcggcggca tcggaaagtc
     2521 ccaaggcgac tgccgccgct tcgcatcgtg cgccggagcc ggccaggagt gcgcacgacg
     2581 agggcggcgc gtcgcagcac gacgcgttcg ccgtgcagct cgcggcattc gccgacgacc
     2641 gcggcgcgaa ctcgctcgcc ggccgcttga agaagtccgg atacgccgca tataccgagc
     2701 ctctgaagac cagcaagggc acgctctggc gcgtgcgcgt gggtccctat ccgtcgcgcg
     2761 acgcggcact cgccgcgcgc gacaagttga agaccgaagg tcagagcggc attgtcgccg
     2821 ccatcaaata gcgtcgccgg acggtggccg tctcgcggcg tgtcggcgac tcccgttcat
     2881 gacgacgttc gactggatcg tggtatgcat cgtggggctg tcgacgctct tcgcgttctt
     2941 tcgcggcgta atccgcgagg tggtcgcgct cgtcgcatgg gttgccggcc tcgtgtgcgc
     3001 gatcctcttc accccgatcc tcggcgcgat gctgcccgac atgttcggcc acccggcgat
     3061 caggtacgtg ctcgcattca cgctgatcct cgttgcggcg ctgctgctcg gtgcgctcgt
     3121 cgcgtggccg ttgtcgacgg tggttcgcgc ggcgggtctt ggtttcgtcg atcgcttcct
     3181 cgggtcgata ttcggcttcg cgcgcggcgt cctgctcatg atcgctttcg tgttcgtcgc
     3241 cggcctgaca ccgcttccgc gcatgctgtg gtggcaaagc gcggcactcg tgccgccgct
     3301 cgtggccggc gtgtatgcgc tgcggccgca tctgccggcg cagatcgccg ggcggctcga
     3361 ttactcgccg caccgcatcg cgccgaaggt cgcgcctgcc gaacagaagg cgtgacgtga
     3421 aacctctccc tcactgacat gaaacgtccc cccacgctcg cttcgctcgc tgccccccgg
     3481 ggggcgggtc agtcgcttgg ggcggcccgg cgcgactgac atgtgcggca tcctcggcgc
     3541 cgttgcgcat accccggtca accagctcct ctacgacggg ctgctgctgc tgcagcatcg
     3601 cggccaggat gcggccggca tcgtgacgag cgaaggcccc gtcttccata cgtacaaggg
     3661 tcccggctac gtgcgcgacg tgttccgcac gcgcaacatg cgcgagctgt cgggcacgac
     3721 cggcatcgcg cactgccgct atcccacggc cggctcggcg ttctccgacc tcgaatcgca
     3781 gccgttctac gtcaattcgc ccttcggctt gacgctcggc cacaacggca acctgaccaa
     3841 cagcgaggcg ctgaagcgcg agctgtggga gctcgacttt cgccacgtca acaccaattc
     3901 cgacagcgaa gtgctgctga acgtgctcgc actcgagctc gagcgcgccg cgcagcatca
     3961 ccgcctcgac cccgaggcga tctttcgcgc ggttgccggc gtgcatcggc gttgccgcgg
     4021 cgcgtacgcc gtggtcgcga tgatcgccgg ctacggcatg ctcgcgttcc gcgatccgtt
     4081 cggcattcgt ccgttgatca tcggcagcaa ccaggcgatg ggtggccccg aatatctggt
     4141 ggcctccgag tcggtcgcgc tcgagccgct cggcttttcg gtgctgcgcg acgtcgagcc
     4201 cggcgaggcg attttcgtcg accaggcggg caccttccac gcgcgccagt gcgccgcgca
     4261 cccgtcgctc aacccatgca tcttcgagta cgtgtacctg gcgcggcccg attccgtgat
     4321 ggacggcacg tcggtgtacg aggcgcggct tcgaatgggt gcagcactgg cgacgaagat
     4381 ccgcgacatc gcgcaggcgc aggacatcga cgtggtgatc ccgatccccg actcgagccg
     4441 gccatatgcg ctcgagctcg cgaaccggct cgggaagacc tatcgcgaag gcttcgtcaa
     4501 gaaccgctac gtcgggcgca cgttcatcat gccggggcag gcgttgcgcc ggaagagcgt
     4561 gcgccagaag ctgaacccga tcggcatgga gttcaaggac aaggtcgtgc tgctcgtcga
     4621 cgattcgatc gtgcgcggta ccacgtcgcg ggagatcgtt cagatggcgc gcgatgccgg
     4681 cgcgcgcaag gtgtatttcg cgtcggcgag cccgccggtg cgcttcccga acgtctacgg
     4741 catcgacatg ccgaaccagg atgagctggt cgccaccgga cgcacggaag ccgagatcgc
     4801 gcgcgagatc ggcgccgacc ttctcgtgta tcaggacctc gaggcgctca aggaagcggt
     4861 gcgcttcggc aatccgaggc tcaccgattt cgaggcgtcg tgcttcgacg gcagatacat
     4921 caccggcgac atcacgtccg actatctgcg agagctggca ctcgagcgcg atcagtcgcg
     4981 cggcgaagcc gacgccgacg tggcggagga tgcaagcgcc ggataagggg tcagagacga
     5041 ctttccgtgc gcgacatgga cgctcacgac tggcgtttgg aaagtcgtct ctgaccccac
     5101 ttgtggtata gttgtggtgc gccgatttga gcttcagctt gcgggcgctt aacaaattcc
     5161 gctaaagcga ggttcgctcc agcccgaaga tatcgggcgg ggcacacaaa acccgtttgg
     5221 cggaaagccc gacgggtttt ttgtttttgc gcgctgcgca aagccacgaa ggacgctgtc
     5281 atgaccgaga accatcacga gcgcggcttc accaccacga tcctccatag cgaccgcgac
     5341 gcggcgatcg agcacggcgc gctgcacaag ccgctgcacg tctcggtggc gtatggctac
     5401 aaggatgcgg cggacctcgt cgccgtgttt cagggccggc aatcgggcta cgcgtacggg
     5461 cgccagggca atccgacgac ggccgccctc gaggcgaagg tcacggcaat ggagaagggc
     5521 gtcgcgacgg tgtgcttttc aaccggcatg gccgcgatcg aggccacgct cgtcgcactt
     5581 ctgcgtggcg gcgatcacat cgtcgcgagc tcgttcgtct tcggcaacac caacagtctg
     5641 ctcaacacgc tcggcgacca cggcacgccg gtcacgttcg tcgatgcgac cgacgtcgcg
     5701 aacgtcgcac gggccgtgac cccggcgacg aggctcgtgt tcgtcgagac gatcgccaat
     5761 ccgcgcacgc aagtcgccga tctcgcgcgc atcggcgagc tgtgccgcga gcgtggcctc
     5821 atctacatcg tcgacaacac gatgacctcg ccgtggctct ttcgcccgat cgacgttcac
     5881 gcgggcctcg tgatcaacgc gctcaccaag tacatcggcg gtcacggcaa cgcgctcggc
     5941 ggcgccgtga ccgacacggg cctctacgac tggacacgat ttcccaacat cgccgagccg
     6001 tacaagacgc agaagccgcc gctgtgggga atcacgcaga tccgcaagaa gggcctgcgc
     6061 gacggggggg cgacgctgtc gcccgagcag gcgcatcacc tggcggttgg cgcggaaacg
     6121 ctcgcgctgc gaatggaccg ccaatgcgcc aacgcgcagg cgcttgccga gttccttgcg
     6181 tcgcatccga aggtcgaggc ggtgtatttc cctggcctgc ggtcgcatcc cgagcacgag
     6241 cggtcgcggt cgctcttccg cgcaccgggc gcgctgcttt cgttcgacct tgcacagggg
     6301 gaggatgtgc tcgcgtgcct caaccggttg cgcgtcgtcg tgctgtcgtc caatctcggt
     6361 gacaaccgca cgctcgccat accggtcgcg cagacgatct tctgggagat gggcccggcg
     6421 cggcgcaccg agatgggcat tgccgattcg ctcgtccgcg tgtcggtagg catcgaggat
     6481 cgcgacgatc tgatcgacga cttccgccgc gcgctgcgtt gagccgcggc aaatggtgca
     6541 tactgcacgc tcgcgtaacg gagaacggcg atggccaatc gcgacaagca gcgcaaggaa
     6601 ccgaagaagc ccaagcagcc gaagaagccc acgcccaagg cgtgacgttc ggccgcttcg
     6661 gcgtcccgtg ccgcgctgcg attcagcgcc gcggcgatcc gtcgcgcgct cttgatctgg
     6721 cgcggcgcaa tatccagatc acgatcggaa tccagcccag ccagcggacg agatcgacga
     6781 tgctttcgtc ccacgtccac gcgccggccg ggtcggcccg cgcagtgagc acatcgtcga
     6841 gccagccgaa gccggtcagc cagacaatga tgccgagcag cagcacaagc tcgaagcgct
     6901 gctggccacg caattggcgc gacacgggga ccgcgacgag tcccccgacg atcaccgcgt
     6961 aaagcagcgc gcgcagcccg cgctcaccgg aaagcacggc ggcgacgagc ggctcgctcg
     7021 caatcatctt ggcgcccgtc cagcccagca cggcggcgcc gagccacagg atggccggat
     7081 agcgctcgac ccacttcagc acgagcgtcg agccccagat gacgatcggc accgacacgc
     7141 cgaggccgat cacgacgagc agcatcgacc cgtgcgcggc acccgccacg gcgagcacgt
     7201 tgtcgacacc catgaccgcg tcggcgacga cgatggtttg cacggcgctg cgcaccgatg
     7261 cctcggcctt gacgtccggc tcgctcttcc cgccatcgtg cgtgagtttc cagccgatat
     7321 agacgagcgc gaggccgccg gcgagcagga agcccggtat tttcagcagc cagacgacca
     7381 cggccgtcag aagcacccgg acggcgattg cgcccgcggt gccccacagg atgaccttgc
     7441 gctgcgcgtg gggcggaacg ttgcgcgcgg cgagtccgat cacgagcgca ttgtccccgg
     7501 cgaggatcaa gtcgatcacg atgatcgagg cgagcgccga gaaccaggcc gcggaaaaca
     7561 gttcgggatt catccgacga tgtgccgcgt gtgcacgcca tcggcatcga tggcgatgta
     7621 atggccgtgc tcgtcccagt ccgcgagcac gatgcgctcg cgcggctgtc cgtcgactcg
     7681 cgtcgagtga cgcgccgggc ggtgcgtatg accgtggatc atccgcgtca ccgcgtgcat
     7741 gcgaaatgcg tgctcgacgg cggcggcgtt gacgtcgagg atcgactcgg gcttgcgcgc
     7801 ggttgccgcg cgactgccgc ggcgcaacca cgccgcgatc cagcgtcgca ccctgtaggg
     7861 caggcggcgc aggcgccgct gcatcgcggg gtcgcgcgtg aagctgcgat aacgctggta
     7921 ggcgtcgtcg tcggtgcaaa gctcgtcgcc gtgcgtgacg agcgtcggcg tgccgccgag
     7981 gtcgacgacc gtttgctcgc cgagcagtgt tgcgccggtg tcgcgcgcga acgcgtcgcc
     8041 gagcaggaaa tcacggttgc cgcgcgcaac gtagagcgcg acgcctgcgt cgctgatgcc
     8101 gcgcaacgcg gcgacgatcg agcgcgcgaa cggttcgcgg cactggtcgt cgccgatcca
     8161 cgaatcgaag agatcgccca tcacgtaaac ggccgcggca gcgcgcgccg gtccccgcgc
     8221 aaacgcttcg aacgccgcgg ccgcggccgg acggtcgggc gccagatgaa ggtcggacaa
     8281 caggagcgtc ggcttcatcg ccgcacgcga acggcgcccc tgcgctggcc gcgggagagg
     8341 tgatcgtgtg tcagccggcg acttcggcgc gcgtgatcac gacgtcctgc gtggggacgt
     8401 tctcgtgaaa gccggagcgt ccggtggcaa cgcccgcgat cctgtcgacg acatcgcttc
     8461 cgcgcacgac cttgccgaac acgcagtacc cgtaaccctg cggactcggc cccttgtagt
     8521 cgaggaaggc gttgtcggcg agattgatga agaactgcgc cgaggcggaa tgcgggtccg
     8581 aggtgcgcgc catcgccacc gagtaccgca cattgcgcac cccgttggcg gcctcgttgg
     8641 ccaccggtgc acgcgtcgcc ctttgttgca tccccggtgc aaagccgccg ccctggatca
     8701 tgaatccggg aatcacgcgg tgaaagagcg tgttgtcgta atggccgtcg cgaacgtatt
     8761 gcaggaaatt ggcaaccgtc gccggcgaat tctcttcgtc gagcgcaagc gcgatgtcgc
     8821 cttcgctcgt gtgcagcacg acctgcgtca tgtt
//
LOCUS       DASSVX010000224        12420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1059253, whole genome shotgun sequence.
ACCESSION   DASSVX010000224 DASSVX010000000
VERSION     DASSVX010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12420
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059253"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1516
                     /locus_tag="VFO53_09710"
     CDS             131..1516
                     /locus_tag="VFO53_09710"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HET8876590.1"
                     /translation="MSEHPPAIEFPEAFLWGSATSAYQVEGSPLADGAGPSIWQRFTR
                     TPGLVRDGDTGDRACDQYRRYADDVALMKRLGMNAYRFSISWSRVLPEGRGAVNAKGL
                     DHYERLVDTLLAHGIAPAPTLFHWDLPAALDDRGGWLNPDIGQWFADYASIVFRRLDD
                     RVPMWATLNEPWVVTDGGYLHGVLAPGHRNVFEAPIAAHQLLRAHATAVQAYRAIGRH
                     RIGIVVNLEPKYPASNSADDRAAASRAHAYMNRQYLDPVFLGRYPKELAQIFGEAWPD
                     WPDGEVEFIRQPIDFVGVNYYTRNVTRFDAASWPLRAASVRQKRATYTETGWEVFPQG
                     LTDALVWVKERYGNPAIYVTENGAAFFDPPVVEGDRLADPLRVDYLRKHLTAVHAARV
                     AGADIRGYFAWSLLDNFEWSLGYSKRFGIVHVDFESQKRTPKESAHFYSRIIASRGRV
                     LNDAPASADDA"
     gene            1645..3576
                     /locus_tag="VFO53_09715"
     CDS             1645..3576
                     /locus_tag="VFO53_09715"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET8876591.1"
                     /translation="MLAAAFIESLALIGTIVPGGTIVFMAGVLAGLHALDPWLAAVLA
                     IAGAILGDGLSYTFGRRYAGTIRATPLLLRYPGLFARGEAYFEANGRKGVFLGRFLGP
                     LRAVVPLVAGMSGMTPRDFFTIDAVSAVAWAAAHLLPGVLFGASLQLAGAVSSRLVVL
                     VALLVAGLWLITLVTRAVTRRGWPYLVRLRERLFVRASAGSGRLPRAVRDLLDPGRHE
                     PLALLISATLLVAGAWLFLGVVEDVVTQDTLVDVDRAIYAWLQGLRTRWADDVMVTIT
                     ELASAQVMIAVIAAVGLWFAITRRFRTLGYWITAASVAEVLVWGLKYGFERARPATPY
                     AAVDEFSFPSGHAAISMVVYGFLAFLIGHRKPVSKQTLLALGAVVIAVLVAFSRLYLG
                     AHWLSDVIASFGLAVAWVALLGIAYIPRVDEGTMRATPVLLIVVATLAFVGGPYVANH
                     HQRDMKRYANSGERPALAIDAWQGGAWSSLPSARTDIGGDREEPFSVQWVATREDITG
                     MLHGAGWVTPAPWRSSAALLWLLPSTPIGELPVLPKLDQGQPPALTFILPRGAQSRTV
                     LRLWHPADAIDGSSRRVPIWIGMITTERARSQWRLIAVTRTEPRLPAPDEALAQAVRG
                     RQTALVRRATNAEAVLLVR"
     gene            3714..4592
                     /locus_tag="VFO53_09720"
     CDS             3714..4592
                     /locus_tag="VFO53_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876592.1"
                     /translation="MKKPRWAIVGVLVTTAMVCSVAADAATSEPKVIVTKPQKVGNGW
                     VHAYVALNAKGRPLALGVSLDNRALEGLPKEPDPTSRCFDRNGNGKIDADECVGDYNF
                     TFVIPQGDAASAIAPFKWVALNWNPHGHSHPAPPPWSAPHFDFHFYVAPIEAVKRLRP
                     GACGEMIDCDDFKAATKPVPARYVHADHIDVGAAVPGMGNHLINSRSPELAKNGPAFT
                     HTFIFGAYDGHITFLEPMVTHAYIASKPDMCAPIKQPEAWEIAGWYPTKYCIRYLEAA
                     ARSTVSIEDFVRRDAR"
     gene            complement(4675..4748)
                     /locus_tag="VFO53_09725"
     tRNA            complement(4675..4748)
                     /locus_tag="VFO53_09725"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4712..4714),aa:Met,seq:cat)
     gene            4846..5619
                     /locus_tag="VFO53_09730"
     CDS             4846..5619
                     /locus_tag="VFO53_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET8876593.1"
                     /translation="MSLSQLLPDVPNHVLLIAPLIVLLGYTIFGATGFGSSIISVPGL
                     AHFFPLTFTVPLITTTDAFAAMSTALRWRRLVAWREFGRLVPAMLIGIALGMTLLLNL
                     PRDPALLALGVFAASYGTYVIIGPPKVARAPAWLVWPVGIIGGIFSALFGTGGPIYIV
                     FLSARIEDKSALRATSAIVVALSIWIRLALFVATGLLLNPTLLAMVLLLLPVMAVGLW
                     LGNRLHHALSRGGVLRLIAGLLIINGVALILRAAESMRA"
     gene            complement(5646..6623)
                     /locus_tag="VFO53_09735"
     CDS             complement(5646..6623)
                     /locus_tag="VFO53_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET8876594.1"
                     /translation="MNRKRTALVAFAIAAGIIALLGAALVLKGLPGRDVVDALGPIAS
                     VPENSADETAGDALVPDDNGSRFLPVPAPLDDERVTDAFGPLAKLSCHRDDPPDTSAD
                     AADADALDAPLASWSAEAQRAGTEPLLAAALAPLTPLPAAQAERLAEAAKVSDDAIPA
                     DCPELLRQQFNRLQTGELQSLCQFRGKVLLIVNTASYCGYTGQYEGLEALYRKYKGRG
                     LVVVGFPSNDFGGQEPGTNKEIGEFCRLTYGVQFPMFEKSSVTKVNSNPLFAALSART
                     GVVPQWNFYKYVVDRHGRAVAGFDSRITPDNPKLVQLIERLLADRVAEG"
     gene            6586..7629
                     /locus_tag="VFO53_09740"
     CDS             6586..7629
                     /locus_tag="VFO53_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET8876595.1"
                     /translation="MANATSAVRFLFIGVLTAREEVRYRFGDRRAIGRLPTMMHAELT
                     DVSQNISAVERETGLSKDVLRMWERRYGFPKPARDDNGERQYSLAETAKLRAIKRLMD
                     IGSRPGKIIGLSLAELNALAEARAPLRRDAAPAAMERDIVSMLRMHDAAVLQHALAQW
                     LMREGLQRFVVETLAPLNRLVGEAWMRGELHIFQEHLYTELVQASLRTAINTFPRHAG
                     HPRVLLTTLPSEQHGLGVLMVEALLVPEGAHCVSLGAQTPLDDIRLATSAHGIDIVAL
                     SFSSAFPVRQAADSLARLRRHLPASVALWAGGEMTRRIRKMLPGVLLLPDLGGSVAAL
                     RGWRASNVNMPMS"
     gene            7660..8604
                     /locus_tag="VFO53_09745"
     CDS             7660..8604
                     /locus_tag="VFO53_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8876596.1"
                     /translation="MWTSISLWLVTLAAAGTYATVAANHISSGRSAALWAAGALLLYF
                     GAILLLCASYFAISWIWRARRPREVRLGVRGTLRLWWHEYRTLAGSPPRLMLYGLLVR
                     DPAPAPVETPVLLVHGVLCNAGVWVRLARYLRKHGVTGLYGLSYGPPLASIDGFAEQL
                     ARKIDAIATATGASRVIIVAHSMGGLVARAHLRRHGAARIARIVTIGSPHHGSMLAWF
                     FPGQSLAQMRPGNPWLAQLNAERLDPALRFVSLWSWHDSMVAPQTSSELPGAVDVALA
                     GIGHNALLGDAGVFALVRKEIEDERRRPPTARRDTCSE"
     gene            complement(8573..9487)
                     /locus_tag="VFO53_09750"
     CDS             complement(8573..9487)
                     /locus_tag="VFO53_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET8876597.1"
                     /translation="MTSARSLPAALALCAVLVVQASGALAQDKSKPAPEPVDTLKRIK
                     DSGAITIGVREASVPFSYIDAQKQPQGYSIDLCLKVADAVKTELKMPRLDVRFVPVTS
                     GNRIAMVKDGKVDLECGSTTNTRDRQKDVAFAYTTFVAGIKMLAKKSSNITSVEDLRG
                     KTVVVTKGTTSEKMLKQLNDDRVLKLTILETTDHNESFAAVADGRAVAFPMDDVLLYG
                     LISKAAKPDDFAVVGRYLSVEPYGIMLRKDDQPFERIVDKTLNELFQSGEVRRIYARW
                     FSTSQLTVPMNQYLKEAFVIPNTYPAWP"
     gene            9542..9739
                     /locus_tag="VFO53_09755"
     CDS             9542..9739
                     /locus_tag="VFO53_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HET8876598.1"
                     /translation="MEKLPDNDPPPVPPRRPDNDECCGGGCNPCIFDAYEDARERYEA
                     ALAAWRARHGVEDDAHDREGR"
     gene            complement(9849..10139)
                     /locus_tag="VFO53_09760"
     CDS             complement(9849..10139)
                     /locus_tag="VFO53_09760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HET8876599.1"
                     /translation="MATIRIDRPHHRSQAGAKTLVEHIARDFEKRFDLAWHWDGDDVH
                     FRRPGVSGLMHVGETNIVLEVRLGLLLAPLKPAIERQMSAHLDRLDDRATRA"
     gene            10275..10532
                     /locus_tag="VFO53_09765"
     CDS             10275..10532
                     /locus_tag="VFO53_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET8876600.1"
                     /translation="MRTELVTTLKRQATELLSELERSKEPILITQHGLPSAYLVDVRT
                     YESISRRMNMLEGIARGEKAIEDGRAVSQAKARQRMARWLK"
     gene            10520..10834
                     /locus_tag="VFO53_09770"
     CDS             10520..10834
                     /locus_tag="VFO53_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET8876601.1"
                     /translation="MAQVIWTEPALADLDAIADYIALDNPAAAKAHVQKVLRRVELLA
                     VHPGRGSKPRELKGWRYRQIIEPACRIFYRQDAARVYIVYVMRAERLLHPRVLTKHSR
                     RS"
     gene            10932..11723
                     /locus_tag="VFO53_09775"
     CDS             10932..11723
                     /locus_tag="VFO53_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8876602.1"
                     /translation="MKPHNGALRESPAGAMAPRTGDRRTWNATKARILDAAESLFMEH
                     GYEATSLRVITASAGVNLAAVHYHFGSKEELFQSVLARRLDPMNRARLDVLTRLEAEA
                     APKPLSCERVLTAFLTPALTLARDPERGGKDFLRLLGRAYADPAPFIRHFLSAQYGVT
                     IARFKAAFARALPQLPARELSWRLHFIMGALSYTLAGTDALKLIAELNPHETTNDEVL
                     LRRLAPFLLAGLTAPLPAPVAVAEAPARVPARAARRRTAAGGNAR"
     gene            11720..>12420
                     /locus_tag="VFO53_09780"
     CDS             11720..>12420
                     /locus_tag="VFO53_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8876603.1"
                     /translation="MNLFVAWLAAVATLVAILVVPLAVPTLRRALISKPLLLRFRRVM
                     PRMSQTEREALEAGTVSFDGELFRGRPAWGQLLSIPRSRLTAEEQHFLDHEVETLCAM
                     VSDWDTTNVYKDLPPQAWQYIREHGFLGMIIPKAYGGLGFSAYAHSEVVTKLSTRCAS
                     VAVTVLVPNSLGPGELLLHYGTDAQKRHYLPRLARGIEIPCFALTNPQAGSDAAAIPD
                     HGIVCYGEHDGARVL"
BASE COUNT         1912 a         4130 c         4149 g         2229 t
ORIGIN      
        1 gcaaatccgt ggccgtgcac ggcgacgagg ttcgcatcga cgaactgcag gcgagggtgg
       61 tgctggaact gccgcgctga gtcaggatcg gccagcgcgc ggcgagacgt tttctatccc
      121 aggaagcgca atgagcgagc acccaccggc gatcgaattt cccgaagcct tcctctgggg
      181 cagcgcgacg tcggcctacc aggtcgaagg ttcgccgctg gccgatggcg cgggtcccag
      241 catctggcag cgatttaccc gcacgccggg cctcgtgcgc gacggcgata ccggcgaccg
      301 cgcatgcgat cagtaccgcc gctatgccga cgatgtcgcg ctgatgaagc gcctcggcat
      361 gaacgcgtat cgcttcagca tttcgtggag ccgcgtgctg ccggagggcc ggggtgcggt
      421 aaacgcgaag ggactcgacc attacgagcg tctcgtcgat acgttgctcg cccacggcat
      481 tgcgcccgca ccgacgctgt ttcactggga tctgccggcg gcgctcgacg atcgcggcgg
      541 ctggctcaat cccgacatcg ggcagtggtt cgccgactac gcgtcgatcg tgtttcgaag
      601 gctcgacgac cgcgtgccga tgtgggccac gctgaacgag ccgtgggtgg tgaccgacgg
      661 cggctatctg cacggcgtcc tcgcgccggg tcaccgcaac gtcttcgagg cgccgattgc
      721 ggcgcaccag cttttgcgcg cgcatgcgac cgcggtgcag gcgtaccgcg caatcggcag
      781 gcatcgcatc ggcatcgtcg tcaacctcga gccgaagtat ccggcgtcca acagcgccga
      841 cgaccgcgcc gcggcgtcgc gtgcgcatgc gtacatgaat cggcagtacc tcgacccggt
      901 gttcctcggc cgctacccga aggagctcgc gcagatcttc ggcgaggcat ggcccgactg
      961 gcccgacggc gaggtcgagt tcatccgcca gccgatcgac ttcgtcgggg tcaactatta
     1021 cacgcgcaac gtgacgcgct tcgacgcagc gtcgtggccg ctgcgcgccg catcggtgcg
     1081 ccagaagcgc gctacgtaca ccgaaacggg ctgggaagta tttccccagg ggcttaccga
     1141 cgcgctggta tgggtcaagg agcggtacgg caatcccgcg atctacgtca ccgagaacgg
     1201 tgcggcgttc ttcgatccgc cggtcgtcga gggcgatcgt ctcgccgatc cgctgcgcgt
     1261 cgactacctt cgcaaacacc tgaccgcggt gcacgcggcg cgtgtggcgg gggccgatat
     1321 ccgcggctac ttcgcgtggt cgctcctcga caatttcgaa tggtcgctcg gctattcgaa
     1381 gcgcttcggc atcgtgcatg tcgacttcga gagccagaag cgcacgccga aggaaagcgc
     1441 gcatttctat tcgcgtatca tcgcgagccg gggacgcgtg ctgaacgacg cgcccgcaag
     1501 cgccgacgac gcgtaagcga accctgttcg acgcgccacg gcgcactccg caggccgaac
     1561 gcatagcatt cagcacgtct ggaagcgcac gttcaatcgc tgatcgactg gttcgcggcg
     1621 cacccggcgc ttgcgctgtc ggccgtgctg gccgcggcat tcatcgagtc gctggcgctg
     1681 atcggcacga tcgttcccgg cggcacgatc gtcttcatgg cgggtgttct cgcgggactg
     1741 cacgccctcg atccctggct tgccgcagtg ctcgccatcg cgggcgcgat cctcggcgac
     1801 ggtctcagct acacgttcgg ccgcagatac gccggcacga ttcgcgcgac gccgctgctg
     1861 ctgcgctatc ccgggctctt tgcgcgcggc gaagcgtact tcgaggcgaa cggccgcaag
     1921 ggcgtcttcc tcggccgctt cctgggaccg cttcgcgccg tggttccgct ggttgccgga
     1981 atgtcgggga tgacgccgcg cgatttcttc acgatcgacg cggtgtccgc agtcgcgtgg
     2041 gccgcggcgc atctgctgcc gggcgtcctc ttcggcgcgt cgctgcagct cgccggtgcg
     2101 gtcagctcgc ggctggtggt gctggttgca ttgctcgttg ccggcctgtg gctgatcacg
     2161 ctcgtcacgc gcgccgtcac gcggcgagga tggccttatc tcgttcgcct tcgcgagcgg
     2221 ctgttcgttc gagcgagtgc aggctcgggg cgcctgccgc gggccgtgcg cgatctgctc
     2281 gacccgggcc ggcacgagcc gcttgcgctt ctgatctccg cgacattgct cgtcgccggt
     2341 gcatggctgt tcctcggcgt cgtcgaggac gtcgtcacgc aggacacgct cgtcgatgtc
     2401 gatcgcgcga tctacgcgtg gctgcagggc ctgcgaacgc gctgggccga cgacgtgatg
     2461 gtgacgatta ccgagctcgc gagcgcgcag gtgatgattg ccgtgatcgc ggcggtcggc
     2521 ctctggtttg cgatcacgcg ccggtttcgc acgctgggct attggatcac cgcggccagc
     2581 gtggccgagg tgctcgtctg ggggctgaaa tacgggttcg agcgcgcgcg gccggcgacc
     2641 ccttacgcgg cggtcgacga gttctcgttc ccaagcgggc atgccgcgat cagcatggtc
     2701 gtgtacgggt tcctcgcatt cctgatcgga catcgcaagc ccgtatcgaa gcagacgctc
     2761 ctggcactcg gcgcggtagt catagccgtg ctcgtcgcgt tctcgcggct ttatctcggt
     2821 gcgcattggc tctcggatgt catcgcgagt ttcgggctcg ccgtggcgtg ggttgcgctg
     2881 ctcggcattg cctacatccc gcgggtcgac gagggaacga tgcgcgccac accggtgctg
     2941 ctgatcgtgg tcgcgacgct ggcgttcgtc ggcgggccgt acgtcgcaaa ccatcatcaa
     3001 cgcgacatga aacgttacgc gaactcgggc gagcgtcccg cgctcgccat cgatgcgtgg
     3061 caaggcggcg cgtggagcag cctgccgtct gcacgcaccg acatcggcgg cgatcgggaa
     3121 gagccgtttt cggtgcaatg ggttgccacg cgcgaagaca tcaccggcat gcttcatggc
     3181 gcgggctggg tgacgccggc gccgtggcga tcgtccgccg cgttgttgtg gctgctgccg
     3241 tcgacgccca tcggcgagtt gccggtgctg ccgaagctcg atcagggaca gccgcccgcg
     3301 ttgacgttca tccttccgcg gggcgcgcaa tcgcgcacgg tgctgcgcct ttggcatccc
     3361 gccgacgcaa tcgacggttc atccaggcgc gtgccgatat ggatcggcat gatcacgacg
     3421 gagcgcgcgc gctcgcaatg gcgattgatc gcggtgacgc gaaccgagcc gcggttgccg
     3481 gcgcccgacg aggcgctcgc gcaggccgtg cgcggaaggc agacggcgct ggttcgacga
     3541 gcgacgaacg cggaggcggt tttgctggtg cgctgacgtc gggtgcctgc tcgtcacggc
     3601 ggcgatggct gcatggcggc gcttgtcgtc ccgcacgggc gagcgtatgt ttctcgcgca
     3661 cctgttcgcg ccaataggcg ccgttcaacg cagcgcatgc acgggagaaa acgatgaaga
     3721 agcctcgatg ggcgatcgtt ggcgtcctgg tcacgaccgc gatggtttgc agcgtcgccg
     3781 cggacgccgc aacttccgag ccgaaggtca tcgtcacgaa gccgcagaag gtcggcaatg
     3841 gatgggttca cgcttacgtt gcgctgaatg ccaagggtcg tcccctcgcg ctgggcgtga
     3901 gcctggacaa ccgggccctc gaagggctgc cgaaagagcc cgatcccacc agccgatgct
     3961 tcgacaggaa cggcaacggg aagatcgacg ccgatgaatg cgtcggcgac tacaacttca
     4021 cgttcgtcat tccgcaaggc gacgcggcga gcgcgattgc acctttcaag tgggtggcgc
     4081 tcaactggaa tccgcatggc cactcgcacc cggcgcctcc accgtggtcc gcgccgcact
     4141 tcgatttcca tttctacgtg gcaccaatcg aggcagtcaa gcggctgcgg cccggcgcgt
     4201 gcggcgaaat gatcgactgc gacgatttca aggccgcgac gaagccggtt cctgccaggt
     4261 acgttcacgc cgatcatatc gatgtaggcg ccgcggtccc gggcatgggc aatcatctga
     4321 tcaattccag atccccggag ctcgcgaaga acggccctgc gttcacgcac acgttcatct
     4381 tcggtgcgta cgacgggcac atcaccttcc tcgagccgat ggttacccac gcctacatcg
     4441 cgagcaagcc cgacatgtgc gcgccgatca agcagcccga ggcgtgggaa atcgccggct
     4501 ggtatccgac gaagtactgc attcgctatc tggaagccgc ggctcgatcc accgtatcga
     4561 tcgaggactt cgtgcggcgt gacgccaggt agggcgtagc aggcgacgtt gcgcgcgcat
     4621 tgctgcggcg agcggaaagg gctcacgcga tcgcaatgag ccctttgaaa cgagtagcga
     4681 gcgagggact cgaacccccg accaaaggat tatgattcct ctgctctaac caactgagct
     4741 agctcgcctt tgcagccgtt attatagcgg caaaccggcc tcgaccggcc cgctgccggc
     4801 cgcttccagc ccgcgatgcg acgccgttcc agcatcgtcg catccatgtc cctttcccag
     4861 ctgctgcccg acgttccgaa tcacgtcctg ctcatcgcgc cgctgatcgt gctgctcggc
     4921 tatacgatct tcggtgcaac cggcttcggc tcgtcgatca tctcggtccc cggtctcgcg
     4981 catttctttc cgctgacgtt cacggtcccg ttgatcacga cgaccgacgc gttcgcggcg
     5041 atgtcgacgg cgctgcgatg gcgccgtctc gtcgcatggc gcgagttcgg gcgcctcgtc
     5101 ccggccatgt tgatcggcat cgcgctcggc atgacgctgc tgctcaatct gccgcgcgac
     5161 ccggcgttgc tcgcgctcgg cgtgttcgcc gcgagctacg ggacgtacgt gatcatcggt
     5221 ccgccgaaag tcgcgcgcgc acccgcctgg ctcgtatggc ccgtcggcat catcggcggc
     5281 atcttttccg cactgttcgg aaccggcggg ccgatctaca tcgtgttcct gtccgcgcga
     5341 atcgaggaca agtcggcgct gcgtgcaacg tccgcgatcg tcgtggcgct cagtatctgg
     5401 atccggctcg cgctgttcgt cgcgaccggc ctgctgctga atccaacgct cctcgccatg
     5461 gtgctgttgc tgctgccggt gatggcggtc ggcctctggc tcggcaaccg gctgcaccat
     5521 gcgctgtcgc gcggcggcgt gctgcggctt atcgcgggtc ttctgatcat caacggcgtc
     5581 gcgctgatcc tgcgcgccgc cgagtcgatg cgtgcctgag accgcgacgt gccgagcctg
     5641 tccggctagc cttcggcgac ccggtcggcg agcaggcgct cgatcaattg caccagcttc
     5701 gggttgtcgg gcgtaatgcg gctgtcgaag cccgcgacgg cgcggccatg gcggtcgacg
     5761 acgtacttgt agaagttcca ctgcggcacg acgcccgtcc tcgcgctcag cgcggcaaac
     5821 agcgggttcg aattcacctt cgtgacactc gacttttcga acatcgggaa ctgcacgcca
     5881 taggtgaggc ggcagaactc gccgatttcc ttgttcgtgc ccggctcctg gccgccgaaa
     5941 tcattcgacg ggaaaccgac gacgacgagc ccgcgaccct tgtacttgcg atacagcgct
     6001 tcgagtccct cgtattggcc ggtgtagccg cagtagctcg cggtgttcac gatcagcagc
     6061 accttgccgc ggaactggca gagcgattgc aattcgccgg tctgcagccg attgaattgc
     6121 tgccgcagca gttcggggca atcggcggga atcgcgtcgt cactcacctt ggcggcctcg
     6181 gccagccgct cggcctgcgc cgcgggaagc ggagtcagcg gggcgagcgc cgctgcgagc
     6241 aacggctcgg tgcccgcgcg ctgcgcttcg gcggaccacg acgcgagcgg cgcgtccaga
     6301 gcatcggcgt cggcggcgtc cgcgcttgtg tcgggcggat cgtcccgatg acacgaaagc
     6361 tttgccagcg gaccgaacgc gtcggtgacg cgttcatcgt cgagcggagc gggcacgggc
     6421 aggaaccggc tcccgttgtc gtcgggaaca agcgcgtcgc cggcggtttc gtcggcgctg
     6481 ttttcgggga cgctggcgat cgggcccagg gcgtcgacga cgtcacgtcc gggaaggccc
     6541 ttcagcacga gcgccgcccc gagcagcgcg atgatgcccg ccgcgatggc gaacgccacg
     6601 agcgcggtgc gcttcctgtt catcggtgtc ctgacggcgc gtgaggaagt acgataccgc
     6661 ttcggcgatc gccgggcaat cggacgactt ccgacgatga tgcatgcaga gttgaccgac
     6721 gtctcgcaga acatcagcgc cgtggagcgt gaaacggggc tctcgaagga cgtcctgcgc
     6781 atgtgggagc gccgctatgg cttcccgaag cccgcccgcg atgacaacgg cgagcgtcaa
     6841 tactcgctgg ccgaaacggc caagctccgc gccatcaagc ggctgatgga tatcggctcc
     6901 cggccgggca agatcatcgg cctttcgctg gccgagctca atgcgctcgc cgaggcgcgc
     6961 gccccgctgc gccgtgacgc cgcgcctgcg gcgatggagc gcgacatcgt atcgatgttg
     7021 agaatgcacg acgctgcggt gttgcagcac gcgcttgcac agtggctgat gcgcgagggt
     7081 ctgcagcgct tcgtcgtcga gacgctggcg ccgctcaacc gcctcgtcgg cgaggcctgg
     7141 atgcgcggcg agctgcacat attccaggag catctttata ccgagctcgt gcaggcgtcg
     7201 ctgcggacgg ccatcaacac gtttccgcgg catgccggac atccgcgcgt gctgctgacg
     7261 acgctgccca gcgagcagca cggcctcggt gtgctgatgg tcgaggcgct gctcgtcccc
     7321 gaaggcgcgc actgcgtttc gctcggagcg cagacgccgc tcgacgacat ccggcttgcc
     7381 accagcgcac acggcatcga catcgtcgcg ttgtcgttct cgagcgcctt tccggtgcgc
     7441 caggccgcgg acagcctcgc gcggctgcgc cggcacctgc cggcgtcggt ggcgctgtgg
     7501 gcgggcggcg agatgacgcg ccgcattcgc aagatgctcc ccggcgtcct gcttctgccc
     7561 gacctcggcg gctcggtcgc ggcattgcgc ggctggcgtg cgagcaacgt caacatgccg
     7621 atgagttgag ccgtccggcg cgcccgtatc atcggcgcga tgtggacgtc gatctcgctg
     7681 tggctcgtga ccctggctgc ggccggcacg tatgcgaccg tcgccgcgaa ccacatctcg
     7741 tcgggccggt cggccgcgct atgggcggcc ggtgcgctgc tcctgtattt cggcgccatc
     7801 ctgctgctgt gcgcgagcta cttcgcgatc agctggattt ggcgcgcgcg gcggccgcgc
     7861 gaggttcggc tgggcgtgcg cggaacgctt cgcctctggt ggcacgagta tcggacgctc
     7921 gcgggttcgc cgccccggct gatgctctac ggactgctcg tgcgcgatcc tgcccctgcg
     7981 ccggtggaaa cgccggtgct gctggtgcac ggcgtgctgt gcaatgccgg cgtgtgggtg
     8041 cgtcttgcgc gctatctgcg caaacatggc gtcaccgggc tttacggcct gtcgtacgga
     8101 ccgccgctcg cgtcgatcga cggcttcgcc gagcagcttg cacgcaagat cgatgcgatc
     8161 gccaccgcga ccggtgcgtc gcgcgtgatc atcgtcgcgc acagcatggg tggcctcgtc
     8221 gcccgcgccc atctgcgcag gcatggagcc gcgcgcatcg cgcgcatcgt cacgatcgga
     8281 tcgccgcacc acggaagcat gctcgcgtgg tttttccccg gacagtcgct cgcgcagatg
     8341 cgtccgggca atccgtggct cgcgcaactc aacgccgagc ggctcgatcc ggcgctgcgc
     8401 ttcgtgtcgc tatggtcctg gcacgattcg atggtcgcgc cgcaaacgtc gtcggaactg
     8461 cccggcgcgg tcgacgtcgc gctggccggc atcggccata acgcgcttct cggcgacgcc
     8521 ggcgtgttcg cgctggtgcg aaaggaaatc gaggatgaaa ggcggcgccc gcctacggcc
     8581 aggcgggata cgtgttcgga atgacgaacg cttccttgag atactggttc atcgggacgg
     8641 tcagctggct cgtcgagaac cagcgtgcgt agatgcggcg gacctcgccg ctctggaaga
     8701 gctcgttcag cgtcttgtcg acgatgcgct cgaacggctg atcgtccttg cgcagcatga
     8761 tcccgtacgg ttccaccgac aggtagcggc cgaccaccgc gaaatcgtcg ggcttcgccg
     8821 ccttcgaaat caggccgtag agcagaacgt cgtccatcgg gaaggcgacg gcgcgcccgt
     8881 cggccactgc ggcgaacgac tcgttgtggt cggtcgtttc gaggatcgtg agcttcagca
     8941 cgcgatcgtc gttgagctgc ttcagcatct tctcgctcgt cgtgcccttc gtgacgacga
     9001 cggtcttgcc gcgcagatcc tcgacgctcg tgatgttcga cgatttcttc gccagcatct
     9061 tgatgcccgc cacgaaggtc gtgtaagcga acgcgacgtc cttctggcgg tcacgtgtat
     9121 tggtcgtcga gccgcattcg aggtcgacct tgccgtcctt caccatcgcg atccggttgc
     9181 cggacgtcac cggaacgaag cgcacgtcca gacgcggcat cttgagctcg gtcttgaccg
     9241 cgtcggcgac cttgaggcat agatcgatcg agtagccctg cggctgcttc tgggcgtcga
     9301 tgtacgagaa cggcacgctc gcttcgcgca cgccgatcgt gatggcgccg ctgtccttga
     9361 tgcgcttcag cgtgtcgacc ggctcgggcg cgggtttgga cttgtcctgc gcaagtgcgc
     9421 cggacgcctg gacgacgagc accgcgcaaa gcgcgagcgc cgccggaaga gagcgtgcgg
     9481 aggtcatggc gcgcgatcat gcaagatcgg cgccagccgt gcgcgtgact ggactgcgac
     9541 gatggagaag cttcccgaca acgatccgcc gccggtcccc ccgcgccggc ccgacaacga
     9601 cgaatgctgc ggcggcggct gcaatccgtg catcttcgat gcttacgagg atgcgcgcga
     9661 gcgctatgaa gccgcgctcg ctgcgtggcg ggcacgccat ggggtcgaag acgacgcgca
     9721 cgatcgcgaa gggcgatgac ggcagccggg tgggtgacaa gtcgtcgcgc tcgcctcgat
     9781 ctgccgcaac gcgtcgcgaa atgccgcttc ggcgccaggg cccgttcgcg tcctttgcgt
     9841 tgactgccct aggctcgcgt cgcgcggtcg tccaggcggt cgaggtgcgc gctcatctgc
     9901 cgctcgatgg ccggcttgag cggtgcgagc agcaggccga gccgcacctc gagcacgatg
     9961 ttcgtttcgc cgacgtgcat gagcccggaa acgcccggtc gccggaaatg cacgtcgtcg
    10021 ccgtcccagt gccaggcgag gtcgaagcgt ttctcgaagt cgcgcgcaat gtgctcgacc
    10081 aatgtctttg cgccggcttg cgaccgatga tgcggccgat cgattcggat cgtcgccatg
    10141 cgcgcatcgt actacggcgg tacgcgcgtc cgcatgcaac gcgccgaacg cctgtcgggg
    10201 tgacacagtc gtgcgagacc gaacctgtgc tgcgagcttg atttgccgcg agtgatactt
    10261 tagtatcact cgctatgcgt accgaactcg tgacaaccct gaaacggcag gcgaccgagc
    10321 tgctgtcgga gctcgagcga agcaaggagc ccattctcat tacacagcat ggcctgccga
    10381 gcgcctacct ggtcgacgtg cggacctacg aatcaatatc gcgtcggatg aacatgctcg
    10441 agggcattgc gcgcggcgaa aaggcaatcg aagacggacg tgctgtttct caagcgaagg
    10501 ccaggcagcg catggcccga tggctcaagt aatctggacc gagccggcgc tcgccgatct
    10561 cgacgccatc gcagactaca tagcgctaga caatcccgcg gcagcgaagg cgcacgtcca
    10621 gaaggttttg cgtcgcgtcg agctacttgc tgtccatccc gggcgcggct cgaaaccgcg
    10681 tgagctcaaa gggtggcggt acaggcagat catcgaaccc gcttgccgaa tcttttatcg
    10741 gcaagatgcg gcgcgtgtct acatcgttta cgtgatgcgc gcagagcggc tgctgcatcc
    10801 tcgtgtgctg accaaacaca gcagacggtc ttgacgggtg ccgcacccaa cgatggaggg
    10861 tgccgaccgc gcttgaccgc gcgcgcgcac cgacgtacga ttcaaacaaa cgtttgaatg
    10921 gccgttcatc gatgaaaccg cacaatggcg cgctgcgtga atcgcctgcc ggcgcaatgg
    10981 cgccgcgcac cggtgaccgc cgcacatgga acgccacgaa ggcgcgcatc ctcgacgctg
    11041 ccgaatcgct gttcatggag catggctacg aggcgacgag cctgcgcgtg atcacggcct
    11101 ccgccggcgt caatctcgcc gcggtccatt atcacttcgg ctcgaaggag gagctcttcc
    11161 agtcggtgct cgcgcgaagg ctcgacccga tgaaccgcgc gcggctcgac gtgctgaccc
    11221 ggctcgaggc cgaggcggcg ccgaagccgc tgtcgtgcga acgggtactc acggcattcc
    11281 tgacgcccgc gttgacgctg gcgcgcgacc ccgagcgcgg cggcaaggat tttctgcgcc
    11341 tgcttggccg cgcctatgcc gaccccgcgc cgttcatccg gcacttcctt tccgcgcaat
    11401 acggcgtcac gatcgcacgg ttcaaggcgg cgttcgcgcg tgccctgccg cagcttccgg
    11461 cgcgcgaatt gtcgtggcgg cttcacttca tcatgggcgc cctgtcgtat acgctcgccg
    11521 gcaccgacgc gctgaagctc atcgccgagc tcaacccgca cgagacgacc aacgacgagg
    11581 tgctgctgcg ccgactcgcc ccgttcctgc tcgccgggct caccgcgcca ttgccggcgc
    11641 cggtcgccgt tgcggaggcg ccggcgcgcg taccggcccg cgccgcgcgg cgtcgcaccg
    11701 cagcaggcgg caacgcccga tgaatctgtt cgtcgcctgg ctcgcggcag tggccacgct
    11761 ggtggcgatc ctcgtcgttc cgttggcggt ccccacgctg cggcgcgcgc tcatcagcaa
    11821 gccgctgctg ttgcgcttcc gccgcgtgat gccgcgcatg tcgcaaaccg agcgtgaagc
    11881 gctcgaggcg gggaccgtga gcttcgacgg cgagctcttt cgggggcgcc cggcgtgggg
    11941 ccaactgctt tcgattccga gatcgagact caccgccgaa gagcagcact tcctcgacca
    12001 cgaggtggag acgctttgcg cgatggtgtc cgactgggac acgaccaacg tgtacaagga
    12061 tctcccaccg caggcgtggc agtacatccg cgagcacggg ttcctcggca tgatcatccc
    12121 gaaggcgtac ggcggcctcg gcttctcggc gtatgcgcat tccgaggtgg tcacgaagct
    12181 gtcgacgcgc tgcgccagcg ttgccgtcac ggtgctcgtg ccgaactccc tcggcccggg
    12241 cgagctcttg ctgcattacg gcaccgatgc gcagaagcgg cattacctgc cacggctcgc
    12301 gcgcggcatc gagattccgt gcttcgcgct cacgaacccg caagcgggct ccgacgccgc
    12361 ggccattccc gaccatggca tcgtctgcta cggcgagcac gacggggcgc gcgtgctctg
//
LOCUS       DASSVX010000225         3801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_225952, whole genome shotgun sequence.
ACCESSION   DASSVX010000225 DASSVX010000000
VERSION     DASSVX010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225952"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1027
                     /locus_tag="VFO53_09785"
     CDS             110..1027
                     /locus_tag="VFO53_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8876604.1"
                     /translation="MALAIALVAFALLPLFLKSYGTYLCTLFCVMLMATCGLNLTVGY
                     AGQMSLGQAAFYGIGAYIAAIALKAGVPFFVVLPVAGIACFAVGLALGFPALRVQHHY
                     LAFATLGFNILVYLVMRNEEKITGGTYGISGIPRPSLFGLSLDGVLPFFWFTLAATVV
                     LMAMLGWLLRSPWGRAFAALRDNPIRAESLGINITAYTLFAFAIGAACAGIAGVFFAA
                     LVQYVEPAPFHFSVSLMMLLAIIVGGAGRFFGPVIGTVVIVLLPEWLRFMQDWYLAVF
                     GFAVVALMIWLPGGILSIPERFARAKRSP"
     gene            1024..1770
                     /locus_tag="VFO53_09790"
     CDS             1024..1770
                     /locus_tag="VFO53_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876605.1"
                     /translation="MSAGFLDVRNVHKAYGAIRAVDGVTFRVGPGEIVGVIGPNGSGK
                     TTLFNSILGQIRPSSGSVAFCGEDITGRAPLELARRGIGRTFQTLQVFVQLSVRDNLI
                     AAAQEFQGTMRSRLFAKPDAGLGARADAMIELFGLTHVARLPAGGLSYGQQKLIDIAM
                     AFMPSPRLVLLDEPCAGVNPGLVDELRELLVTLNRTQGGSFVLIEHNMDFIMKLCPHV
                     ICMVEGRVMAEGAPRVVQSDPRVLEAYLGN"
     gene            1760..2536
                     /locus_tag="VFO53_09795"
     CDS             1760..2536
                     /locus_tag="VFO53_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876606.1"
                     /translation="MATETASAATTPLAPIIEFDDVVAGYSPSLTILNGTTFDVRRSE
                     ITMLIGPNGAGKSTVLKTLFGVLTPRTGEIRFEGARINGRTPRELLAQGIAFVPQGRN
                     LFASLSVRHNLELGGITLPRADLAARIDEVLVRFPRIGERLDSQASTLSGGEQKQLEV
                     GRALLLRPRVLLIDEPSIGLSPKLVQQVMTLLRALADAGTTVLMVEQNVRTALKYADR
                     ALVLEMGRLALDRPASALLHDPDLNRLFLGGHVEASSGAR"
     gene            2596..3798
                     /gene="pcaF"
                     /locus_tag="VFO53_09800"
     CDS             2596..3798
                     /gene="pcaF"
                     /locus_tag="VFO53_09800"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HET8876607.1"
                     /translation="MGEAFICDAVRTPFGRYGGSLSGMRTDDLAAVPLRALVERNPGV
                     DWGRVDDVVYGCANQAGEDNRNVARMSLLLAGLPQEVAGTTVNRLCGSSLDAVTIAAR
                     AIKADEMSLVVAGGVESMSRAPFVMGKAAKAFERAATLEDTTLGWRFVNRAFKARYGV
                     DSMPETAENVAREFAISRADQDAFALRSQQRAAGAIVDGRMAEEIVPVVIAQDKGDSR
                     VVTKDEHPRATSLEALARLEGIVRPDGTVTAGNASGINDGSCALVVASEEAAKRNGLT
                     PRARVVAGAVAGVAPRIMGFGPAPAMRKVLALARLTLADIDVIELNEAFAAQALAVMR
                     DHGLADDDARVNPTGGAIALGHPLGASGGRLVTTALRQLQRTRGRYALCTMCIGVGQG
                     IALVIERV"
BASE COUNT          550 a         1276 c         1281 g          694 t
ORIGIN      
        1 gaattgcgcc cccgcggggg gcagcgacgc ggcgcagccg caagcgtcgg ggggtcccgc
       61 aagcgctgcg ccgtcgcccg tttcggatcg cgcgtttcgc gccaggtgca tcgcgctcgc
      121 gatcgcgctc gtcgcctttg cgctgctgcc gctgtttctc aaaagctacg gtacttatct
      181 ctgcacgctc ttctgcgtca tgctgatggc gacgtgcggg ctcaatctca cggtcggcta
      241 tgcgggccag atgtcgctcg ggcaggccgc cttttacggc attggcgcct atatcgccgc
      301 gattgcgctc aaggccgggg tgccgttctt cgtcgtactg cccgtggccg gcatcgcctg
      361 cttcgccgtg ggcctcgcac tcggttttcc cgcgcttcgc gtgcagcacc attacctcgc
      421 gttcgcgaca ctggggttca acatcctcgt gtacctcgtc atgcgcaacg aggaaaagat
      481 caccggcggc acgtacggga tttcgggaat cccgcgtcct tcgctgttcg ggctttcact
      541 cgatggcgtg ctgcccttct tctggttcac gctcgcggcg accgtcgtgc tgatggcgat
      601 gctcggctgg ctcctgcgct cgccgtgggg ccgtgcgttc gcggcgctgc gcgacaatcc
      661 gatacgcgcc gaaagcctcg gcatcaacat caccgcctac acgctgtttg ccttcgcgat
      721 cggtgccgct tgtgcgggca ttgcgggcgt gttcttcgcc gcactcgtgc agtatgtcga
      781 gcccgcgccg tttcatttct cggtatcgct catgatgctg ctcgcgatca tcgtcggcgg
      841 cgccgggcgc ttcttcgggc ccgtcatcgg cacggtcgtc atcgtgctcc tgcccgaatg
      901 gctgcgcttc atgcaggact ggtacctggc ggtattcgga ttcgccgtcg tcgcgctgat
      961 gatctggctg cccggcggaa tcctctcgat tccggagcgc ttcgcccgcg cgaaaaggtc
     1021 gccgtgagtg ccggcttcct cgacgtgcgc aacgtgcaca aggcctatgg cgccatccgc
     1081 gccgtcgatg gcgtgacctt tcgcgtcggg ccgggcgaga tcgtcggcgt gatcggaccg
     1141 aacggctcgg gcaagacgac gctcttcaac agcatcctcg gccagatccg gccgtcgtcg
     1201 ggcagcgtcg cattctgcgg cgaagacatc acgggcaggg ctccgctcga gctcgcgcgg
     1261 cgcggcatag gccggacgtt tcagacgctg caggtgttcg tgcagctctc ggtgcgcgac
     1321 aacctgatcg ccgcggcgca ggagttccag ggcacgatgc gttcgcggct gtttgcgaag
     1381 cccgacgcgg gcctcggcgc gcgcgccgac gcgatgatcg agctcttcgg actcacgcac
     1441 gtcgcgcgtc ttccagcagg cggcctttcg tacggccagc agaagctcat cgacatcgcg
     1501 atggcgttca tgccgtcacc gcggctcgtg ctgctcgacg aaccctgcgc cggtgtcaat
     1561 cccggcctcg tcgacgagct gcgtgagctg ctcgtcacgc tgaacaggac gcagggaggc
     1621 agcttcgtgc tgatcgagca caacatggat ttcatcatga agctgtgtcc gcacgtgatc
     1681 tgcatggtcg aaggcagggt gatggccgag ggcgcgccgc gcgtcgtgca gtccgatccg
     1741 cgcgtattgg aggcgtacct tggcaactga aacggccagc gcagccacaa cgccgctcgc
     1801 gccgatcatc gaattcgacg acgtcgtcgc cggctattcg ccgagtctga cgattctcaa
     1861 cggcacgacg ttcgacgtgc ggcgcagcga gatcacgatg ctgatcgggc cgaacggcgc
     1921 cggcaagtcg acggtgctga agacattgtt cggcgtgctg acgcctcgca ccggcgagat
     1981 ccgcttcgaa ggcgcgcgca tcaacggcag gacgccgcgc gagcttctcg cgcagggcat
     2041 cgccttcgtg ccgcaggggc gcaatctttt cgcgtcgctt tcggtgcggc acaatctgga
     2101 gcttggcggc atcaccctcc cgcgcgccga tcttgcggcc cgcatcgacg aagtgctggt
     2161 gcgcttcccg cgcatcggcg agcgcctcga cagccaggcg tccacgctgt ccggcggcga
     2221 gcagaagcag ctcgaggtcg gccgcgcact gctgctgcgc ccgcgcgttc tgctgatcga
     2281 cgagccgtcg atcgggctgt caccgaagct cgtgcagcag gtgatgacgc tgcttcgcgc
     2341 gctcgccgat gccggaacca ccgtgttgat ggtcgagcag aacgtgcgca ctgcgctcaa
     2401 gtacgcggat cgcgcgctcg tgctcgagat gggccgtctc gccctcgatc gcccggcatc
     2461 cgcgctgctg cacgaccccg atctcaatcg gttgtttctc ggcggccacg tcgaagcgtc
     2521 cagcggcgcg cgataatggg cggtgcggcg cgatgcatgc gtttcgcgcg gcttttttgc
     2581 cggtaaagga aatcgatggg cgaggcgttc atctgcgacg cggtcaggac accgttcggc
     2641 cggtatggcg gttcgctgtc cggcatgcgc acggacgacc ttgcggcggt cccgttgcgc
     2701 gccctggtcg agcgcaatcc cggcgtcgac tgggggcgcg tcgacgacgt cgtctacggc
     2761 tgtgcgaacc aggcgggcga ggacaatcgc aacgtcgcgc gcatgtcgct cctcctcgcc
     2821 ggcttgccgc aggaagtcgc tggcacgacc gtcaaccgcc tgtgcggctc gagcctcgac
     2881 gccgtgacga ttgcggcgcg tgcgatcaag gcggacgaaa tgtcgctcgt ggtggcgggt
     2941 ggcgtcgaaa gcatgtcgcg cgcgccattc gtcatgggca aggcggcgaa ggcatttgag
     3001 cgagccgcga cgctcgagga cacgaccctc ggctggcgct tcgtcaaccg cgcgttcaag
     3061 gcgcgctacg gtgtcgattc gatgcccgaa acggccgaga acgtcgcgcg cgagttcgcg
     3121 atatcccgcg ccgaccagga cgcattcgcg ctgcgcagcc agcagcgtgc ggccggcgca
     3181 atcgtcgatg gccggatggc tgaagagatc gttcccgtcg tgattgccca ggacaagggc
     3241 gattcgcgcg tcgtgacgaa ggacgagcat ccgcgcgcca catcgctcga ggcgctggca
     3301 agactcgaag gcatcgtgcg gcccgatgga acggtgaccg ccggcaatgc gtcgggcatc
     3361 aatgacggtt cgtgcgcact cgtcgtcgcg tccgaggagg cagcgaagcg caacggcctc
     3421 acgccgcgcg cccgcgtcgt cgccggggcc gtggcaggcg ttgcgccgcg gatcatgggc
     3481 ttcggcccgg cgcccgccat gcgcaaggtg ctggcactcg cgcgactcac gctcgccgat
     3541 atcgacgtga tcgagctcaa cgaagccttc gccgcccagg cgctggccgt catgcgcgat
     3601 cacggtctcg ccgacgacga cgcgcgggtc aatcccaccg gaggagccat tgcgctcggc
     3661 catccgcttg gcgccagcgg cggccggctc gtcaccaccg cgctgcgcca gttgcaacgt
     3721 acgcgcggcc gctacgcctt gtgcacgatg tgcatcggcg tggggcaggg catcgcgctc
     3781 gtcatcgagc gcgtttgatc a
//
LOCUS       DASSVX010000226         5815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_626131, whole genome shotgun sequence.
ACCESSION   DASSVX010000226 DASSVX010000000
VERSION     DASSVX010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626131"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFO53_09805"
     CDS             complement(<1..397)
                     /locus_tag="VFO53_09805"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET8876608.1"
                     /translation="MRRFIANPDFRAGFVDMLPATLALVPFGLVCGVGAAAAGASLAG
                     AVGLSAIVFSGAAQILAVQLLAAGAPLAVIVLTCFVAGLRFLIYSAAMAPYLRPLPPR
                     WQKGLAFLLTDQAFAVAVRRFGAGGDACAA"
     gene            complement(479..979)
                     /locus_tag="VFO53_09810"
     CDS             complement(479..979)
                     /locus_tag="VFO53_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HET8876609.1"
                     /translation="MKHAMRNAAITAAVAAALGMVAGVTFAADKKTAVAAHDHGRYVV
                     TIGGCNDCHTPGYAMSGGKVDEKQWLTGDSLGWRGPWGTTYPINLRLYMQAISEKEWV
                     DKARHLTARPPMPFWALNQMTDADLRALYRYIRGLGPAGHPAPDYVPPTQEPKPPYVT
                     FPAPPK"
     gene            1083..2039
                     /locus_tag="VFO53_09815"
     CDS             1083..2039
                     /locus_tag="VFO53_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HET8876610.1"
                     /translation="MRILELATAAASLAIAPELGGAIVRFAADGKPVLRPASDAALDA
                     RDVRRTACYPLVPYSNRIRNAELDFAGRRYQLERNFGTHPHAIHGVGWQRAWEVAEAS
                     LVHARLTLHHDALGDNGRAWPWAFEATQAFHLADVVSGEHRRSTSLVVTLTLKNTSGA
                     AFPFGLGFHPFFPKATATRLCFDAERVWENDATQLPRALVGVPPQWQFGRGRALDAIA
                     LDNVFTGWGRSATLVDDARGTTVRIDADRALAYVVVYAPADANVVAIEPVTHETDAFN
                     RSAAGATGTGMRVLPPGGAFSCTMRVKAAAAASLAASPDPVR"
     gene            2036..2920
                     /locus_tag="VFO53_09820"
     CDS             2036..2920
                     /locus_tag="VFO53_09820"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET8876611.1"
                     /translation="MTADSTAFSCVLDVRASLGECPLWSIAEQALYWIDINAPSLNRF
                     DPLIGENVAMPMPESIGCYAFRDGGGFVVALRSGVWLCNPDGRLVRRVADAPYDTTHF
                     RFNDGRCDLQGRFVVGTMNEKRDANGAALYRLDGDFALTRLFGGMTISNGLAWSPDGR
                     TMYHADTPAQTIRAFDYDEASGTPSHARAFAHWTGAGERPDGGTVDRAGHYWAAHYGG
                     GKLVELAADGVTVAEHPLPAMCPTMCAFGGADLRTLFVTTARQKRDAAELARFPQSGG
                     IFAMRVETPGMPEAQVRA"
     gene            2927..5149
                     /locus_tag="VFO53_09825"
     CDS             2927..5149
                     /locus_tag="VFO53_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET8876612.1"
                     /translation="MQLDPAHYLRLDVPHSLGATAAGAAFATSSGDVLDVECFGAGMF
                     RLRVGPSTRPDYGILAARPKACTIAQRAPGSWTFSAGDSALELRGSPLTLRVLWKDHV
                     VLRSITDEKLDGGARLPTLGRLRQGGLWSAAFALTTGEPVYGLGEQFGPLNKRGQLIH
                     SHVQDAQGVNTGRAYKGTPFAWSTGCGRGAWGTFVHTPGMVTHGVGHPDWSHRSYAMM
                     VEDEALDLFVFAGESPGAIVDLYTQLTGRPAAVPRWSLGLWVSRASYGSADAAIGVAT
                     RLRERKIPSDVLMLDAGTTSDPNTRFDFTWDAQRIDDPVDTLAKIKAHDFRVCISECP
                     HVSVDSPLFQDLASQQYLLLTPEGDPYVHAWSDAAQPDRAAPPMPESGIVDFTNPDAF
                     AWWRDEHLALFEDGVDVIASEYGEQVPDDAVAFNGDSGARLHNVYPLLYNRCVYEATA
                     RFQKPSDGPPMIWSRAGWAGSQRYPIGFGGSPQSDWEGLSASVRGALSLGMSGNPYHS
                     SDVGGFYGSSQPSAELFVRWLQAAVFFSHMRLNGAGEREPWAFGAEAEGIARKWLALR
                     YRLIPYLEQAIGQARQTGLPVSRAMPLAFPDSRLARDFETQFMCGDALLVAPIVREGG
                     EVDIALPPGAWYDLNTRARYPGSQVLRYAAKLDQFPVFGREGFALPLGRAVQHTGEIN
                     VEVPLEALWLFGKPAQPLAGYRQVSVAADRLTGHTVRAMLNVDVQVFGDASAVNVLPL
                     "
     gene            5149..>5815
                     /locus_tag="VFO53_09830"
     CDS             5149..>5815
                     /locus_tag="VFO53_09830"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET8876613.1"
                     /translation="MGTHPTVAITSGEPAGIGPELVARLAERHRAQPFPARLVALGDF
                     DLLAARAERIGAAARYVRYQPLAFAPLGNAMEVWDQPLAAPAVPGHPDPTNARSVLSM
                     LETGCDACATGAFAALVTGPVQKSVFQDAGIAFMGHTEFFAARTGTPRVVMMLVSGSG
                     PDALRVALVTTHLPLRAVADAITQRAVEETLAIVRHELSARFGVAEPRIAVCGLNPHA
                     GEGG"
BASE COUNT          874 a         1988 c         2002 g          951 t
ORIGIN      
        1 ccgcggcgca tgcgtcgccc cccgcaccga agcggcgtac cgcaacggca aacgcctggt
       61 cggtcaacag gaacgcgagt cctttctgcc agcgcggcgg caacggccgc aggtacgggg
      121 ccatcgcggc gctgtagatc aggaagcgca gcccggcgac gaagcaggtg aggacgatca
      181 ccgcgagcgg ggcgcccgcg gcgagcagtt gcacggcgag gatctgcgcg gcaccggaga
      241 acacgatcgc cgacagaccg accgcgccgg cgagcgaagc accggcggcc gccgccccga
      301 cgccgcacac gaggccaaaa ggcacgagcg ccagcgttgc cggcaacatg tcgacgaatc
      361 cggcgcgaaa atcgggattg gcgatgaagc ggcgcattgc gggttgcgcg ttgcccgcgc
      421 aaaaaaatcg ggaggcgaga cgcctccctg ccgcgcgatt atagccgccg ctcgtgcatc
      481 acttcggcgg cgcgggaaac gtcacgtagg gcggcttcgg ctcctgggtc ggcgggacgt
      541 agtcgggcgc agggtgaccc gcgggtccaa gtccccggat ataacggtag agcgcgcgca
      601 gatcggcgtc ggtcatctgg ttgagcgccc agaacggcat cggcggacgc gcggtcaggt
      661 ggcgcgcctt gtcgacccat tccttttcgg aaatcgcctg catgtagagg cgcaggttga
      721 tcggatacgt ggtgccccat gggccacgcc atccgagcga gtcgccggtc agccactgct
      781 tttcgtcgac cttgccgccg ctcatcgcgt atcccggcgt gtggcaatcg ttgcacccgc
      841 cgatcgtcac cacgtagcgc ccatggtcgt gtgccgcaac ggccgtcttc ttgtcggccg
      901 caaacgttac gccggcgacc atgccgagcg ctgccgccac ggcggccgtg atcgccgcgt
      961 tgcgcattgc gtgcttcatg ctgttctcct cgattgaccg gcggccgagc cgccgctgcg
     1021 gggacggata gtcgcgcagt tcgtacaatg tccgcaagtg cccgacgcaa ggccggcctc
     1081 cgatgcgcat tctcgaactc gcaacagccg ccgcaagcct ggcgatcgcg cccgagcttg
     1141 gtggcgcaat cgtgcgcttc gcggccgatg gcaagccggt gctgcgcccg gcctccgatg
     1201 ccgcgctcga tgcgcgcgac gtgcgccgca ccgcctgcta tccgctggtt ccgtactcga
     1261 atcgcatccg caacgccgag ctcgacttcg ccggacggcg ctatcaactg gagcgcaact
     1321 tcggcacgca tccgcatgca atccacggcg tcggctggca gcgcgcgtgg gaagtggcgg
     1381 aggcatcgct cgtccacgcc cggctcacgc tgcaccatga tgcgctcggc gacaacggtc
     1441 gcgcctggcc gtgggcgttc gaggcgacgc aggcgtttca tctcgccgac gtcgtatccg
     1501 gcgagcatcg gcgttccacc tcgctcgtcg tgacgctcac gctgaagaac acgtcgggcg
     1561 cggcctttcc attcggactc ggctttcatc cgttttttcc gaaggcaacg gccacgcgtt
     1621 tgtgcttcga cgccgagcgc gtttgggaaa acgatgcgac gcagctgccg cgagcactcg
     1681 ttggcgtgcc tccgcaatgg caattcggcc gcgggcgggc gctcgatgcg atcgcgctcg
     1741 acaacgtctt caccggctgg ggccggagcg cgacgctcgt cgacgatgcg cgcggcacca
     1801 cggtgcgaat cgacgccgat cgtgcactcg cgtacgtcgt cgtctatgcc cccgcggatg
     1861 cgaatgtcgt cgccatcgag ccggtgacgc acgaaacgga cgcgttcaat cgcagtgcgg
     1921 cgggcgccac gggaacgggg atgcgcgtgc ttccgcccgg cggtgccttt tcttgtacga
     1981 tgcgcgtgaa ggcggccgcc gccgcctccc tggccgcctc tcccgatccc gttcgatgac
     2041 cgccgattcc actgcgtttt cgtgtgtgct cgacgtgcgt gcaagcctcg gcgaatgtcc
     2101 gctgtggtcg atcgccgagc aggcgctgta ctggatcgac atcaacgcgc cgtcgctcaa
     2161 ccgcttcgac cctttgatcg gcgagaacgt ggcgatgccg atgcccgaat cgatcggatg
     2221 ctacgcgttc cgcgacggcg gcggattcgt cgtcgcgctg cgcagcggcg tctggttgtg
     2281 caatcccgac gggcggctcg tgcgtcgcgt cgcggacgcg ccctacgaca cgacgcactt
     2341 tcgcttcaac gacggccgct gcgatctgca gggccgtttc gtcgtcggca cgatgaacga
     2401 gaagcgcgat gcgaatgggg cggcgctgta ccgactcgac ggcgatttcg cgctgacgcg
     2461 cctctttggc ggcatgacga tcagcaacgg cctggcgtgg agtcccgacg ggcgcacgat
     2521 gtatcacgcc gacacgcctg cgcaaaccat tcgcgcgttc gactacgacg aggcgtccgg
     2581 gacgccgagc catgcgcgcg cgttcgccca ctggaccggc gcgggcgagc gcccggatgg
     2641 cggcacggtc gaccgcgccg gacattactg ggcagcgcac tacggcggcg gcaagctggt
     2701 cgagctcgca gccgacggag tcaccgtcgc cgagcatccg ctgcccgcga tgtgcccgac
     2761 gatgtgcgcg ttcggcggcg ccgacctgcg cacgctgttc gtgacgaccg cgcggcagaa
     2821 gcgcgacgcg gcggagctgg cgcgattccc gcagagcggc ggcatcttcg cgatgcgcgt
     2881 cgaaacgccg gggatgcccg aggcacaggt gcgcgcgtaa cggccgatgc agctcgaccc
     2941 agctcactac ctgcgcctcg atgttccgca cagcctcgga gccaccgcgg ccggtgcggc
     3001 gtttgcaacg tcgagcggcg acgttctcga cgtcgaatgc ttcggcgcgg gcatgtttcg
     3061 tttgcgcgtc gggccgagca cgcgtcccga ttacggcatc ctcgcggcgc gcccgaaggc
     3121 gtgcacgatc gcgcagcgcg caccggggtc gtggaccttc agcgccggcg attcggcgct
     3181 cgaattgcgc ggctcgccgc tgacgcttcg cgtgctgtgg aaggatcacg tcgttctgcg
     3241 ctcgatcacc gacgaaaagc tcgacggcgg cgccaggttg ccgacgctcg gccgcctgcg
     3301 acagggcggc ctgtggtcgg ccgcgttcgc gctcacgacc ggtgaaccgg tgtacgggct
     3361 cggcgagcaa ttcggaccac tgaacaagcg cggacaactc attcattcgc acgtgcagga
     3421 tgcgcagggc gtcaacaccg ggcgcgcgta caagggaacg ccgttcgcgt ggagcaccgg
     3481 atgcggcagg ggtgcctggg gcacgttcgt tcacaccccc ggaatggtca cccacggtgt
     3541 cggccacccc gactggtcgc atcgcagcta cgcgatgatg gtcgaagacg aagcgctcga
     3601 ccttttcgtg tttgccggcg aatcgccggg cgcgatcgtc gatctctaca cgcagttgac
     3661 gggccggccc gccgccgtgc cgcgctggag cctcgggctc tgggtgtcgc gtgcctcgta
     3721 tggcagcgcc gacgcggcaa tcggcgtcgc gacgcggctg cgggaacgca agatcccgtc
     3781 ggatgtgttg atgctcgatg cgggcacgac gtccgatccg aacacccgct tcgatttcac
     3841 gtgggacgcg cagcgcatcg acgatcccgt cgacacgctg gcgaagatca aggcgcacga
     3901 cttccgggtg tgcatatcgg aatgcccgca cgtgtcggtc gactcgccgc tgttccagga
     3961 cctcgcatcg cagcaatacc tgttgctgac tccggaaggc gatccgtacg tgcacgcgtg
     4021 gagcgacgct gcgcaacccg accgcgctgc gccgccgatg cccgagagcg ggatcgtcga
     4081 cttcaccaat cccgatgcgt tcgcgtggtg gcgcgacgag cacctcgcgc tgttcgagga
     4141 tggcgtcgac gtcatcgcga gcgaatacgg cgagcaggtg cccgacgatg cggttgcgtt
     4201 caacggcgat tccggcgcac ggctgcacaa cgtgtatcca ctgctctaca accgctgcgt
     4261 ttacgaggcg acggcgcgtt tccagaagcc gtccgacggc ccgccgatga tctggagtcg
     4321 cgccggctgg gcgggcagcc agcgctaccc gatcggcttc ggcggctcgc cgcaaagcga
     4381 ttgggagggc ctgtcggcat cggttcgcgg tgcgctttcg ctcggcatga gcggcaatcc
     4441 gtatcacagc tccgacgtcg gcggcttcta cggctcgtcg cagccgtcgg cggagctgtt
     4501 cgtgcgctgg ctgcaagccg cggtgttctt ttcgcacatg cgcctgaacg gcgccggcga
     4561 gcgcgagccg tgggccttcg gcgcggaggc cgagggcatt gcacgcaaat ggctcgcact
     4621 gcgttaccgg ttgatcccgt atctcgagca ggcgatcggg caggcgcgac agacgggtct
     4681 gccggtgtcg cgggcgatgc cacttgcgtt tcccgacagc cgcctcgcgc gcgacttcga
     4741 gacgcaattc atgtgcggcg atgcgttgct cgtcgccccg atcgtgcgcg aaggcggtga
     4801 ggtcgatatc gcgctgcctc ccggcgcgtg gtacgacctc aacacgcgcg cccgctaccc
     4861 gggctcgcaa gtgctgcgct atgcggcgaa gctcgaccag ttcccggtgt ttggccgcga
     4921 aggcttcgcg ttgccgctcg gtcgggcggt ccagcacacc ggcgagatca acgtcgaggt
     4981 tccgctcgaa gcactctggc tgttcggcaa gccggcgcaa ccgctcgccg ggtaccggca
     5041 ggtaagcgtc gcagccgacc gcctcaccgg gcacacggtg cgcgcgatgc tgaacgtcga
     5101 cgtgcaggtc ttcggcgatg cgtccgcggt gaacgtcctt ccgctttgat gggcacgcac
     5161 cccacggtcg cgatcacctc cggggaaccc gccggcatcg gtcccgagct cgtggcgcgg
     5221 ctcgccgaac gccaccgcgc acagcccttc cccgcgcggc tcgtcgcgct cggcgatttc
     5281 gatctgctcg ccgctcgcgc cgaacgcatc ggcgccgccg cccggtatgt gcgctaccag
     5341 ccgctcgcgt tcgcgccgct cggcaacgcg atggaagtat gggatcagcc gctggctgcg
     5401 ccggcggtgc ccggacatcc cgacccgacc aacgcgcgga gcgtgctgtc gatgctcgag
     5461 accgggtgcg atgcgtgtgc aacgggcgcg tttgccgccc ttgtcacggg gccggtgcag
     5521 aaaagcgttt tccaggatgc cggcattgcg ttcatggggc acaccgagtt cttcgccgcg
     5581 cgcaccggga cgccgcgtgt ggtgatgatg ctggtgagcg gcagcggccc cgatgcgctg
     5641 cgcgtcgcgc tcgtgacgac gcatctgccg cttcgggccg tggcggatgc gatcacgcaa
     5701 agggctgtcg aggagacgct cgcgatcgtg cggcacgaat tgtcggcgcg cttcggcgtc
     5761 gccgagccgc gcatcgccgt gtgcggtctc aatccgcacg ccggcgaagg cggcc
//
LOCUS       DASSVX010000227        15113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_909594, whole genome shotgun sequence.
ACCESSION   DASSVX010000227 DASSVX010000000
VERSION     DASSVX010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 15113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15113
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909594"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="VFO53_09835"
     CDS             <1..1722
                     /locus_tag="VFO53_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8876614.1"
                     /translation="LKPALARGELHCVGATTLDEYRKYIEKDAALERRFQKVMVDEPS
                     VEDTIAILRGLQEKYEVHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAAR
                     IKMEIDSKPEAMDRLDRRMIQLKIEREAVKKEKDEASRKRLNAIEDEIKRLEREYADL
                     DEVWQAEKAQVAGSQHIKEEIDKIKLQMDEAKRKGDWQKMSELQYGQLPQLEAQLRKA
                     DTRLAIEQEKKPQLLRTQVGAEEIAEVVSRATGIPVSKMMQGERDKLLQMETRLHQRV
                     IGQNEAVRLVSDAIRRSRSGLSDPNRPYGSFLFLGPTGVGKTELTKALAEFLFDSDQA
                     MVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILFDEIEKAHPDV
                     FNVLLQVLDDGRLTDGQGRTVDFRNTVIVMTSNLASHRIQQMTDNGDNPELIKIAVMA
                     EVKATFRPEFVNRIDEIVVFHPLAQAQIASIARIQLRALEQRLSKLDIRLEVAEPALE
                     HVAQAGFDPVYGARPLKRAIQQEIENPLAKEILSGAFAAGDTIHVREKGGHIVFDKSV
                     EPQAVHA"
     gene            complement(1888..2433)
                     /locus_tag="VFO53_09840"
     CDS             complement(1888..2433)
                     /locus_tag="VFO53_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876615.1"
                     /translation="MAATFVARANSRTIDWGIAILVGIVAIVVFAVIEMAFSWAMRGA
                     SPWQPLVVFGTVALHALMPSAHVGGGIRTMLVGAALLLALGALSGIILAYIVDRIGVA
                     GAAIAGAIFGLAMYAVDLYGVARLLPALLDLRDWMSALAYVLQGALTAALYKVMTRDE
                     APAASADVGHDLRDLRDVRLV"
     gene            complement(2582..2983)
                     /locus_tag="VFO53_09845"
     CDS             complement(2582..2983)
                     /locus_tag="VFO53_09845"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876616.1"
                     /translation="MGAVAITTLAGAHLIAASPDYAFLADTGHFLAAFTLFFWATASW
                     WIPLLVVVFAWRHLHQRTPIRYDAQYWSLVFPLGMYSVATRDYAQQTRLAFLYPVATC
                     AAYIALVAWLLTAAGLVHRLLQRPGEASASR"
     gene            2967..3503
                     /locus_tag="VFO53_09850"
     CDS             2967..3503
                     /locus_tag="VFO53_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876617.1"
                     /translation="MATAPIVDPVRGRQHVRAHRSKRPAEEDEPDDEDVEHRAGEKKH
                     EAREHHDVGPRKRARRHRPQHRDRFRGYRREKPGAVQPMLEPRFGLHDGDGGKERVDE
                     DRPQHEASNPERRADRERTGERHALRAEHAGAGNDRQERGPGDMVTERARHGAWRMRE
                     PRNAGKPQQRVGDEVDGE"
     gene            complement(3686..4072)
                     /locus_tag="VFO53_09855"
     CDS             complement(3686..4072)
                     /locus_tag="VFO53_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HET8876618.1"
                     /translation="MPLVTVTLQKPRSAEATSAILDGVHKALVANGVPETDRFQRVLE
                     LDSRTFRYDATYPDVATARGAEFVLIEILWSVGRSAKIKRKVTADIIAALARDPGLDA
                     EQVMIVFVETAWENWAFAGGRLLHAG"
     gene            complement(4093..4431)
                     /locus_tag="VFO53_09860"
     CDS             complement(4093..4431)
                     /locus_tag="VFO53_09860"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HET8876619.1"
                     /translation="MACFESGTIVSLPAREAITLPRVGGTTLRVTQGVVWLTEENDRH
                     DVVLRPGDNWLVESDGDTVVEAQNDAMFCVLGRKAESLKLPSRVQPRSKFARTLASFL
                     VAPARRVPYF"
     gene            4576..5490
                     /locus_tag="VFO53_09865"
     CDS             4576..5490
                     /locus_tag="VFO53_09865"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET8876620.1"
                     /translation="MHRTLRALPSLDFLRGFEAAGRRLSFTLAAQELFVTQSALSRQI
                     KALEDALGIAFFERRHRALALTAAGAVFHRDVTEALEMLAAAAARLHESTRTPGLTLS
                     TTVSFASLWVIPRLATFRARYPDVEVYVSADDRVVDLARGDVDIAVRYLPDSGAPANA
                     LRLFGERMTPVASPKIAFGASPLRAPADLTRHVLLHLDDPEGRTPWLDWRSWLTSNGQ
                     PGLKPAGALRFRIYDQVVQAAVGGQGVALGRLPMIAEHLRDGRLVAPFPKKYESARSY
                     FALAAARAAERDDVAAFLGWLTEEAARE"
     gene            5487..6047
                     /locus_tag="VFO53_09870"
     CDS             5487..6047
                     /locus_tag="VFO53_09870"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8876621.1"
                     /translation="MNGHRPLAPSPWICRFAPLLPPGAHVLDVAAGHGRHARWLAAQG
                     ARVHAVDIDADALGTLAGVGGVATQVADLEGGPWPFADATYDAIVVVHYLHRPAFAEL
                     LRVLKPDGALLYETFATGNEAFGRPSNPAFLLRENELLDIVRERLFVVAFEQGRVDGD
                     RVAVMQRIAAIGRARGWPPPLPAQAG"
     gene            6129..7037
                     /gene="dapA"
                     /locus_tag="VFO53_09875"
     CDS             6129..7037
                     /gene="dapA"
                     /locus_tag="VFO53_09875"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET8876622.1"
                     /translation="MLSGSLVAIVTPMQPGGALDLEALRRLIDFHVANGTAGIVIVGT
                     TGESPTVDVDEHCRLIQCAIEHAAGRLPVIAGTGGNSTAEAIALTRFAKGAGATACLS
                     VVPYYNKPTQEGMYRHFRSIAESVDLPVLLYNVPSRTVADLSTDTVLRLAQVPGIAGM
                     KDATSDLVRLVDLQNRLPAGHEFALYSGNDDTALAFMLLGGNGVVSVTANVAPKQVSE
                     VCEAALAGDVVTARRRNARLSGLHARLFVEANPIPVKWALAEMGLIHNALRLPLTPLS
                     PQHHDAVRTALRDAQCIGRPELKVYS"
     gene            7037..8176
                     /gene="bamC"
                     /locus_tag="VFO53_09880"
     CDS             7037..8176
                     /gene="bamC"
                     /locus_tag="VFO53_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HET8876623.1"
                     /translation="MNPSISAARACALFLVVMLSLAGCESMPSLSKRIDYKSSASATP
                     SLELPPDLTAPQYDDRYAVSSASELAAKDATRPKQSELLPVNSEAHVARAGTQRWLVV
                     NATPEQAWSTVRKFWTDSGFVIATEQPAVGIMETDWAENRAEIPQDILRKYIGKYVDV
                     FYSTYKRDKFRTRIERGADPGTVEIYVSHRGMEQVPTTKIDNVQGAGFAWGLMPPNPG
                     LEAEMLSRLMMRFGTPEAVAEAATSPSAAASAPQHARLEKADGLSKLVVDDPFDRAWR
                     RVGLALDRTGFTVVDRDRSSGTYFVRFADPDTEMARKDREKGFLSKLFTFWKKDDDKE
                     KPEQYRIKVAEASPQSTVVVEDTTGKPDRSPASDRILALLRDQLK"
     gene            8195..8959
                     /locus_tag="VFO53_09885"
     CDS             8195..8959
                     /locus_tag="VFO53_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8876624.1"
                     /translation="MRFASLGSGSEGNALVVEVGDTRILIDCGFRVRDTVARLLRLGV
                     VPESVSAILVTHEHADHIGGVASFAARYRIPVWLTFGTLEMASERIGRLQHVFGFDSH
                     DTFAIGMIEVRPFPVPHDAREPVQFVVGDGAHRLGVLTDIGATTPHVEASLTGCDALV
                     LECNHDLGMLEGSDYPWPLKQRIASRLGHLHNEAAAALLAALDTSRLQHVVAAHLSQQ
                     NNTPERARAALSQAINCAPDWIGIADQHDGFAWRGL"
     gene            8984..9706
                     /gene="purC"
                     /locus_tag="VFO53_09890"
     CDS             8984..9706
                     /gene="purC"
                     /locus_tag="VFO53_09890"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET8876625.1"
                     /translation="MEKRQELYRGKAKTVYATDDPARLVMHYRDDVSAFDGAKVAKLD
                     GKGETNNRINGFVMGKLAAAGIPTHFVSLLNERESLVKAMKMIPVECVVRNICAGSMA
                     KRYGIDEGRRLPEPIFEFFLKSDALHDPLVNDDHIRMLEWATTGEIAQMKALTHRVNA
                     VLKPLFLAADIDLVDYKLEFGHPLDDLRGELVLGDEFTPDGCRLWDARTGEKLDKDRF
                     RRDLGEVIEHYREVGRRIGVPL"
     gene            complement(9886..10242)
                     /locus_tag="VFO53_09895"
     CDS             complement(9886..10242)
                     /locus_tag="VFO53_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HET8876626.1"
                     /translation="MRQAFKMKLLAGLFVPALALAAGTAYATGTDKMKGDASANATTT
                     TPATPSSSMDNGMKSGTSSADARFARLDTNHDGKLSSSEVAADTKVHAMWKKLDTNND
                     GTVSKSEFEAHASDLE"
     gene            complement(10390..10920)
                     /locus_tag="VFO53_09900"
     CDS             complement(10390..10920)
                     /locus_tag="VFO53_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876627.1"
                     /translation="MASSSTPPDTPLPPPPGLPREARLDSLADLDRAHDEVIALAQRH
                     IKIFDTDLSGGRWNTADRCDALSSFLRRMPGARLDIIVHDTRWIESSGARLCALLVRH
                     AHAMKVYRTGAAARAAMDPLVIVDDLHFVHRHHIDWPHGSLSIGSPERAKALVDRFDE
                     IWATGEPGMSANVLGL"
     gene            complement(10895..12028)
                     /locus_tag="VFO53_09905"
     CDS             complement(10895..12028)
                     /locus_tag="VFO53_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8876628.1"
                     /translation="MHRALRQLLGGHAPAAFLRDYWHKKPLLVEAALPGFPALLTRAE
                     LFALAARDDVQSRLVQRAGRRYTQHDGPFKRTQLERLPARNWTLLVQGVNLQHDGADA
                     LLRRFAFIPYVRLDDLMVSYAVPGGGVGPHYDSYDVFLLQASGRRRWRYGRQQDLRLV
                     ENAPLRILARFAPEHAATLAPGDMLYLPPDHAHDGVALDECMTYSIGFRAPAHQELAE
                     AFLDHLRDTLDVSGRYADPGLRPAKAPARIERTMQRRVARALAAIRWNDDDIARFLGR
                     YLTEPKPEVVFTAAAHCSRARFARRMARVGVRLDRRTQLLYDSARCYINGDELPLPAS
                     GADDLRTLADRRTLPPRVFARLSPELVAILYDWHRHGFLVDTA"
     gene            12166..12639
                     /locus_tag="VFO53_09910"
     CDS             12166..12639
                     /locus_tag="VFO53_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET8876629.1"
                     /translation="MNVFANTVVTLSFQLYDAGNKLIEETDEPIAYLHGGHSGIFPKV
                     EAALDHKEVGDSVSVTLEPDDAFGDYDAGLMRIESVEQLPPDIEVGGHLVAEQDGNEM
                     VWRITDIAEGKAVLDGNHELAGQRLRFDCKVLDIRPATQEEITHGHVHGAHGHHH"
     gene            12675..13898
                     /locus_tag="VFO53_09915"
     CDS             12675..13898
                     /locus_tag="VFO53_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET8876630.1"
                     /translation="MIRYHNHPSGRHFLQIPGPTNVPDRVLRAIDRPTIDHRGPEFAQ
                     LGLSILGRIGSIFQTREPVVIYAASGTGAWEAALVNTLSPGDVVLMAETGHFALLWKR
                     LAERLGLQPEFLPGDWRHGADPGAIHARLADDHTHAIRAVCIVHNETSTGVVSNIGRV
                     REAIDRAGHPALLLVDTISSLGSIDYRHDEWGVDVTVAGSQKGLMLPPGLAFNAVSEK
                     ARAASKQAKLPRAYWAWDEIIAANRNGYWPTTPATNLLYGLDAALDQLLDEGLPNVFA
                     RHARHGEATRAAARAWGLEILCANPDEYSNSLTAVLMPEGHDADAFRRVVLERFDMSL
                     GTGLSKLAGRVFRIGHLGDFNDLTLMGTLAGVEMGLRVAGVPHRAGGVDAAMAILGGN
                     RAPVGPKASRAVELA"
     gene            13915..14685
                     /locus_tag="VFO53_09920"
     CDS             13915..14685
                     /locus_tag="VFO53_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET8876631.1"
                     /translation="MTAAPLPEYLAPLRDALVDARHGRIGVAAADLPVPRCDEDAYAV
                     QESVADALGWFAAAPARAWKVGAASRAALPNAAPLPSQHVVDSPATFPAGAFDRMLIE
                     GEIAFRLRAPIDGDDGASVISSIGAWLVTIEVVDPRYADWDTASPMQKLADQGLHGAL
                     VVGSGTALPSAIDWTSLVARVRRNGGIVRETRGGHPLGNLLFLLPWLARHAAARGTPL
                     CAGDVVTAGTWTGVFEAFPGETIDVEFDGVGRASARFD"
     gene            complement(14716..>15113)
                     /locus_tag="VFO53_09925"
     CDS             complement(14716..>15113)
                     /locus_tag="VFO53_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET8876632.1"
                     /translation="SPGMLRKARVRGVYDVLVEGELVDFLRGATARFDVIACADTLCY
                     FGDLAPAIDAAASALRGGGWFGFTVERADDIDTYRINPHGRYSHARRYVERLLAAANL
                     RALDVAPAVLRREAGAEVNGWVVRAMRAP"
BASE COUNT         2569 a         5009 c         5132 g         2403 t
ORIGIN      
        1 ctcaagcccg cgctcgcgcg cggcgagctg cattgcgtgg gcgccacgac gctcgacgaa
       61 taccgcaagt acatcgagaa ggatgccgcg ctcgagcgcc gcttccagaa ggtcatggtt
      121 gatgagccct cggtcgagga caccatcgcg atccttcgcg gcctgcagga gaagtacgaa
      181 gtccaccacg gcgtggacat cacggacccc gcgatcgtgg ccgccgcgga gctttcgcac
      241 cgctacatca ccgaccggtt cctgcccgac aaggcgatcg atttgatcga cgaggccgcg
      301 gcgcgcatca agatggagat cgattcgaag ccggaagcga tggacaggct cgaccggcgg
      361 atgatccagc tcaagatcga gcgtgaagcc gtcaagaagg aaaaggacga ggcgtcgcgc
      421 aagcggctca atgccatcga ggacgagatc aagcggctcg agcgcgagta cgccgatctc
      481 gacgaggtgt ggcaggccga gaaggcgcag gtcgccggca gccagcacat caaggaggag
      541 atcgacaaaa tcaagcttca gatggacgag gcgaagcgca agggcgactg gcagaagatg
      601 tccgagctgc agtacgggca gttaccgcag ctcgaggcgc agttgcgcaa ggccgacacc
      661 cggctcgcga tcgagcagga gaagaagccg cagttgctgc gcacgcaggt cggcgccgag
      721 gaaatcgccg aggtcgtttc gcgcgcaacc ggcatcccgg tgtcgaaaat gatgcagggc
      781 gagcgcgaca agcttctgca gatggagacg cggctgcatc agcgggtgat cggccagaac
      841 gaggcggtgc gcctcgtttc cgatgcgatc cggcgctcgc gttccgggct ctcggatccc
      901 aatcgcccgt atgggtcttt tttgttcctc ggcccgacgg gtgtcggcaa gaccgagctc
      961 acgaaggcgc ttgccgagtt cctgttcgac agcgaccagg cgatggtgcg catcgacatg
     1021 tcggaattca tggagaagca ctcggtcgcg cggctgatcg gtgcgccgcc cggttacgtc
     1081 ggctacgagg agggcggcta tctgaccgaa gccgtgcgcc gcaagccgta ctcggtgatt
     1141 ctgttcgacg agatcgagaa ggcgcacccc gacgtgttca acgtgctgct gcaggtgctc
     1201 gacgacggcc gcctgaccga tgggcagggc cgcaccgtcg actttcgcaa cacggtgatc
     1261 gtgatgacgt cgaacctcgc gtcgcacagg atccagcaga tgaccgacaa cggcgacaat
     1321 cccgagctca tcaagatcgc ggtgatggcc gaggtgaagg cgacgttccg gcccgagttc
     1381 gtgaaccgca tcgacgagat cgtcgtgttc catccgctcg cgcaggcgca gatcgccagc
     1441 atcgcgcgca tccagttgcg tgcgctcgag caacgtcttt cgaagctcga catccggctc
     1501 gaggttgcag agccggcgct cgagcacgtc gcgcaggcgg gcttcgatcc ggtgtatggc
     1561 gcgcggccgc tcaagcgcgc gatccagcag gagatcgaga acccgctcgc gaaggaaatc
     1621 ctgtcaggcg cgttcgccgc cggcgacacc attcacgtgc gcgagaaggg tggccacatc
     1681 gtgttcgaca aaagcgtcga gccgcaggcg gtccacgcct gacgagatcg gggtcagagc
     1741 tgtaattcgc ggcgcgtcgt tatgagatcg gggtcagagc tgtaattcgc gatccattgg
     1801 cggcgttcta acgcacaatg cgccgcgaat tacagctctg accccgatct cgaattacag
     1861 ctctgacccc gatctcacca acgcggccta caccagccgc acgtcgcgca aatcgcgcag
     1921 gtcgtgtccg acgtccgcgc tcgcagcggg cgcttcgtcg cgcgtcatca ccttgtacag
     1981 cgcggcggtg agggcaccct gcaacacata ggccagtgcg ctcatccagt cgcgcaggtc
     2041 gagcagcgcc gggagcagcc gcgcgacgcc gtacaggtcc accgcgtaca tcgcaagacc
     2101 gaagatcgcc cccgcgatgg ccgcccctgc cacgccgatg cgatcgacga tgtacgcgag
     2161 gatgatgccc gaaagcgcgc cgagcgccag cagcagcgca gcgccgacaa gcatcgtcct
     2221 gatgccgcct cccacgtgcg ccgacggcat cagcgcgtgc agggccaccg tcccgaacac
     2281 gacgagcggc tgccatggcg acgcgccgcg catcgcccac gaaaatgcca tttcgatgac
     2341 ggcgaatacg acgatcgcga caatgcccac gaggatcgca atgccccaat cgatggttcg
     2401 gctgttggcc cgtgcaacaa acgttgctgc catgatctcc tccgcgggat aggttgcgtc
     2461 gtgctaacga cgcagattcc ataccgggac gatccagcct gccgctccag cgcagcgcat
     2521 gagcgaggtg tcggcagcct gcaacaaatg ctcgcccgcg aagcgcgtta cgagcctgct
     2581 gtcagcgcga ggcggacgct tcgccgggcc gttgcagcaa tcgatgcacg aggcccgcgg
     2641 cggtgagcag ccatgcgacg agcgcgatgt acgccgcgca cgtcgcgacc ggatagagga
     2701 aggcgagccg cgtctgctgc gcatagtcgc gcgttgcgac gctgtacatg ccgagcggga
     2761 acacgagcga ccagtattgc gcgtcgtagc gtatcggcgt tcgctgatgc aggtgccgcc
     2821 atgcgaagac caccacgagc agcggaatcc accacgaggc ggtcgcccag aagaacagcg
     2881 tgaacgcggc caggaaatga ccggtgtccg cgaggaaggc gtagtcgggt gacgccgcga
     2941 tcagatgcgc tcccgcgagc gtggtgatgg cgaccgcgcc cattgttgat ccagtacgtg
     3001 ggcgtcagca tgtgcgcgcc catcggtcga aacgtccagc ggaagaagat gagcccgatg
     3061 acgaggatgt agagcatcgc gccggcgaga aaaaacacga ggcacgcgaa catcacgatg
     3121 tcgggccgcg gaaacgcgct cgccgtcatc gtccccagca ccgcgaccgc ttccgtggat
     3181 accgtcgcga gaagccagga gccgtccagc ccatgctcga gccgcggttt gggctccacg
     3241 atggtgacgg cggcaaagaa cgcgtagacg aggatcgccc acagcacgag gccagcaacc
     3301 cagagcgccg cgccgaccgc gagcgaaccg gcgagcgcca cgcattgcgc gccgagcacg
     3361 caggtgccgg caacgatcgt caggaacgcg ggcccggtga catggtcacc gagcgagcgc
     3421 gccacggcgc gtggcgaatg cgcgagccgc gcaatgccgg caagccacag cagcgcgtag
     3481 gcgacgaggt tgacggcgaa tagcgcgtgc gcaaccgccg ggagcccctg cgcctgcatg
     3541 gcgagcgata cgatgccggt cgccatgacg aacgcgaagc cgcccgggaa gctgcggcgc
     3601 agcgattcga cgatcgtcgg cgggttggtg gcgagcaggt cttgcggcaa atgcgtgagc
     3661 ttacatcggc cgcgatcgtt gccggttatc cggcgtgcag cagccgcccg ccggcgaatg
     3721 cccagttctc ccacgcggtt tcgacgaaga cgatcatcac ctgctccgca tcgagcccgg
     3781 gatcgcgcgc gagggcggcg atgatgtccg ccgtcacctt gcgcttgatc tttgcgctgc
     3841 gtccaaccga ccacaggatc tcgatcagca cgaactcggc gccgcgcgcc gtcgcgacgt
     3901 cggggtaggt cgcgtcgtag cgaaacgtgc gcgaatcgag ctcgagcaca cgctggaatc
     3961 gatcggtctc gggcacgccg ttggcgacca gcgctttatg cacgccatcg aggatggccg
     4021 acgtcgcctc ggcgcttcgc ggcttctgca gggtgacggt gacgagtggc atcgaatctc
     4081 cccgggttcg gatcagaagt agggaacgcg ccgcgcgggc gcgacaagga acgacgcgag
     4141 cgtcctcgcg aatttgctgc gcggttgaac acgcgacggc agcttcagcg attcggcttt
     4201 gcggcccagg acgcagaaca tggcgtcgtt ttgcgcctcg acgaccgtat cgccgtccga
     4261 ctcgacgagc cagttgtcgc cgggacgcag cacgacgtcg tgccggtcgt tctcctcggt
     4321 gagccagacg acgccttgcg tgacgcgcag tgtcgtgccg cccacgcggg ggagcgtaat
     4381 cgcttcgcgc gcagggagac tgacgatcgt tccggattcg aagcaggcca tggcaaacct
     4441 ccagtagaat gcgtacaggt cattcgacat ccccgaggca ccggaggcac cccacggaat
     4501 cgacgactac gattctgcgg cgccgcccgc cgtcgctcaa acgctcaatt cgaatggata
     4561 gcatgcatgg aacgcatgca tagaacgctc cgcgcgttgc cgtcgctcga ttttcttcgc
     4621 ggcttcgagg ccgccggacg ccgcctttcg ttcacgctcg cggcacagga gctcttcgtc
     4681 acgcaatcgg cactgtcgcg ccagatcaag gcgctcgagg acgccctcgg catcgcgttc
     4741 ttcgagcgcc ggcaccgcgc gctggcgctg accgcggcgg gtgcggtatt tcatcgcgat
     4801 gtgaccgagg cgctcgaaat gctcgcggcg gccgcggcac ggctgcacga gtccacgcgc
     4861 acgccggggc tcacgctgtc gacgacggtg tcgttcgcgt cgctctgggt gattccacgt
     4921 cttgcgacct ttcgcgcgcg ctatcccgat gtcgaggtct acgtgtcggc cgacgatcgc
     4981 gtcgtcgatc ttgcgcgcgg cgacgtcgac atcgccgtgc gctacctgcc cgattcgggc
     5041 gcccccgcga atgcgctgcg ccttttcggc gaacggatga cgccggtggc aagcccgaag
     5101 atcgcgttcg gcgcgtcgcc gcttcgcgca ccggccgacc tgacgcgcca cgtcctgctg
     5161 catctcgacg atcccgaggg gcgtacgccg tggctcgact ggcgcagctg gctcacgtcg
     5221 aacggccagc ccggattgaa gccggcgggt gcgctgcgct ttcgcatcta cgatcaggtc
     5281 gtgcaggccg ccgtcggcgg acagggcgtg gcgctcggcc gtctgccgat gattgccgag
     5341 catcttcgcg acgggcggct cgtcgcgccg ttcccgaaga agtacgaatc cgcgcgaagc
     5401 tacttcgccc tcgctgccgc ccgggcggcc gagcgcgacg acgttgcggc gttcctcggc
     5461 tggctgaccg aagaggcggc acgcgaatga acggacatcg cccgctcgcg ccctcgccgt
     5521 ggatctgccg cttcgcgccg ctcctgccgc cgggagcgca cgtgctcgac gtcgccgcgg
     5581 gccacgggcg tcatgcacga tggcttgccg cgcaaggcgc gcgcgtccat gcggtcgaca
     5641 tcgacgccga cgcgcttgga acgcttgccg gcgtcggcgg cgtcgccacg caggtggccg
     5701 acctcgaagg cgggccgtgg ccattcgccg atgccacgta cgatgcgatc gtggtcgtcc
     5761 actacctgca tcggcccgcg tttgcagagt tgctgcgcgt gctgaagccc gatggcgcac
     5821 tgctctacga gacgttcgca accggcaacg aagcgttcgg gcggccatcg aatcccgctt
     5881 tcctgctgcg cgaaaacgag ttgctcgaca tcgtccgcga gcggctgttc gtcgtcgcgt
     5941 tcgagcaggg acgcgtcgac ggcgatcgcg tcgcggtgat gcagcgcatt gccgcgatcg
     6001 gccgcgcacg cggatggccg ccgccgctgc ccgcgcaggc aggctgacgc tgcgccgatt
     6061 tgcgccaggc acgcgcatgg cgcacgaggc gaatcgggta gaatccccgc ttttgcagga
     6121 aacggttcat gctttcaggc agtctcgtcg cgatcgtgac gccgatgcag ccgggcggcg
     6181 cgctcgatct cgaggcgctt cgccggctga tcgacttcca cgttgcgaac ggcaccgccg
     6241 gcatcgtcat cgtcggcacg acgggcgagt cgcccaccgt cgacgtcgac gagcattgcc
     6301 ggctcatcca gtgcgccatc gaacacgccg caggccggct gccggtcatc gccggtaccg
     6361 gcggcaattc gacggcggag gcgatcgcgc tcacgcgctt tgcgaagggg gcgggcgcga
     6421 ccgcgtgcct gtcggtcgtg ccgtactaca acaagccgac gcaggaaggc atgtaccggc
     6481 acttccgctc gatcgcggag agcgtcgacc tgcccgtgct cctgtacaac gtgccgagcc
     6541 gcacggtcgc cgatctgtcg accgacaccg tgctgcggct tgcacaggtt ccgggcatcg
     6601 ccggaatgaa ggatgcgacg tccgacctgg tacgtctcgt cgatttgcag aaccggttgc
     6661 cggccgggca tgaattcgcg ctttattccg gcaacgacga cacggcgctc gccttcatgc
     6721 tgctcggcgg caacggcgtc gtctcggtca cggccaacgt cgcgccgaag caggtcagcg
     6781 aggtgtgcga agccgcgctt gccggtgatg tcgttaccgc ccgccggcgc aatgcgcggc
     6841 tctccggcct gcatgcgcga ttgttcgtcg aagcgaaccc gatcccggtc aagtgggcgc
     6901 ttgccgagat gggattgatc cataatgcgc tgcggctgcc attgacgccc ttgtcgccgc
     6961 agcaccacga cgccgtgcgc acggcgctgc gcgacgcgca gtgcatcggc cggcccgaac
     7021 tcaaggttta ttcctgatga atccatcgat ttccgcggcg cgcgcctgcg cgctctttct
     7081 cgtcgtcatg ctgtcgctgg ccggttgcga atcgatgccg tcgctcagca aacgcatcga
     7141 ctacaagtcc tcggcgagtg ctacgccatc gctcgagctg ccgcccgatc tcaccgcgcc
     7201 gcaatacgac gatcgttacg ccgtgtcgtc cgcgtcggag ctcgcggcga aggatgcgac
     7261 gcgtccgaag cagtcggagc tgctgccggt gaacagcgaa gcgcacgtgg ctcgcgccgg
     7321 cacgcagcgc tggctcgtcg tgaacgcgac gcccgagcag gcgtggtcga cggtgcgcaa
     7381 gttctggacc gactcgggct tcgtcatcgc caccgagcag ccggcggtcg gcatcatgga
     7441 aaccgactgg gccgagaacc gcgccgagat cccgcaggac atcctgcgca agtacatcgg
     7501 caagtacgtc gacgtgttct actcgacgta caagcgcgac aagttccgca cgcgcatcga
     7561 gcgtggcgcc gatcccggca ccgtggaaat ctacgtgtcg caccgcggca tggagcaggt
     7621 gccgacgacg aaaatcgaca atgtgcaggg cgcgggtttc gcgtggggcc tgatgccgcc
     7681 gaatccgggt ctcgaggccg agatgctgtc gcggctgatg atgcgcttcg gcacgcccga
     7741 agccgtggcc gaggcggcca cttcgccgag tgcggcagcg agtgcgccgc agcacgcgcg
     7801 gctcgaaaag gccgacggcc tgtcgaagct cgtcgtcgac gatccgttcg atcgcgcttg
     7861 gcgccgtgtc ggcctcgccc tcgatcggac ggggttcacc gtcgtcgatc gcgaccggtc
     7921 cagcggcacc tacttcgtgc gcttcgccga tcccgacacg gagatggcgc ggaaggatcg
     7981 cgaaaagggc ttcctgtcga agctcttcac gttctggaag aaggacgacg acaaggagaa
     8041 gccggagcaa taccggatca aggtggcgga agcgtcgccg caaagcacgg tcgtcgtcga
     8101 ggacaccacc ggcaagcccg atcggtcgcc cgcgagcgat cgcatccttg cgttgctgcg
     8161 cgaccagctc aagtaacagg cgctttcgcg cgccatgcgc ttcgcctccc tgggcagcgg
     8221 cagcgaaggc aacgcactcg tcgtcgaagt cggtgacacc cgcatcctga tcgactgcgg
     8281 cttccgcgtg cgcgacaccg tcgcgcgcct gctgcgtctc ggcgtcgtgc ccgaatcggt
     8341 cagcgcgatc ctcgtcacgc acgagcacgc cgatcacatc ggcggcgtcg cctcattcgc
     8401 cgcgcgctac cggattcccg tgtggcttac gttcggcaca ctggaaatgg caagcgagcg
     8461 aatcggccgg ctgcagcacg tattcggctt cgacagccac gacacgttcg ccatcggcat
     8521 gatcgaggtg cggcccttcc cggtgccgca cgatgcgcgc gagccggtac agttcgtcgt
     8581 tggcgacggc gcgcatcgcc tcggcgtgct gaccgacatc ggcgcgacga cgccgcacgt
     8641 cgaggcgagc ctcaccggct gcgacgcgct cgtgctcgag tgcaaccatg acctcggcat
     8701 gctcgagggc agcgactacc cgtggccgct caagcagcgg atcgcgagcc gcctcggcca
     8761 tctgcacaac gaggcggcgg ccgcgctgct cgccgcgctc gacacgtcgc gcctgcagca
     8821 cgtcgtcgcc gcgcacctgt cacagcagaa caacacaccc gaacgggcgc gcgcggcgtt
     8881 atcgcaggcg atcaattgcg cgcccgactg gatcggcatc gccgaccagc acgacggctt
     8941 tgcatggcgc gggttgtagg gacttttgga ggagcaggca gacatggaga agcgccagga
     9001 gctgtaccgt ggcaaggcga agaccgttta cgcgacggac gatcctgcgc ggctcgtaat
     9061 gcactaccgc gacgacgtgt cggcgttcga cggcgcgaag gtggcgaagc tcgacggcaa
     9121 gggcgagacg aacaaccgga tcaacgggtt cgtcatgggc aagctcgccg cggcgggcat
     9181 accgacgcac ttcgtgtcgc tgctgaacga gcgcgaatca ctcgtcaagg cgatgaagat
     9241 gatccccgtc gagtgcgtcg tgcgcaacat ctgcgccggc tcgatggcca agcgctacgg
     9301 catcgacgag ggcaggcggt tgccggagcc catcttcgaa ttcttcctga agagcgatgc
     9361 gctgcacgat ccgctcgtca acgacgatca catccgcatg ctcgagtggg ccacgacggg
     9421 cgagatcgcg cagatgaagg cgcttacgca ccgcgtcaac gccgtgctga agccgctctt
     9481 cctcgcggct gacatcgacc tcgtcgacta caagctcgag ttcggccatc cgctcgacga
     9541 cctccgcggc gagctcgtgc tcggcgacga gttcacgccc gacggatgcc ggctgtggga
     9601 tgcgcgtacc ggggagaagc tcgacaagga tcgcttccgg cgcgacttgg gcgaagtgat
     9661 cgagcactat cgcgaggtgg gacggcgcat cggcgtgccg ctgtaacaag gcgccgctcc
     9721 cgcaaggcat tccctgcggg accgcgatgg gcgcagcggc ggactgcgcc aggcagcttg
     9781 cggagcgcta caagcacaac ggcggactgc gccaggcgcc gcccgccgtt gccgatcgtt
     9841 gcgccacgcg ctggcgcaac gattacgtgc cacttcgagt acgtgttact cgaggtccga
     9901 cgcgtgggct tcgaactcgg acttgctgac cgtaccgtcg ttgttcgtgt cgagtttctt
     9961 ccacatcgca tgcaccttcg tgtcggcggc cacttcggaa ctgctcagct tgccgtcgtg
    10021 gttggtgtcg agccgcgcaa acctcgcatc ggcgctcgac gttccgctct tcatgccgtt
    10081 gtccatgctg ctgctcggcg tggccggcgt ggtggtcgtc gcgtttgccg acgcatcgcc
    10141 cttcatcttg tcggtccccg tcgcataggc ggtgcccgcg gcgagggcga gtgccggcac
    10201 gaacaggccg gccagcagtt tcatcttgaa tgcttgtctc atcggtttct ccttggagga
    10261 agggatcgag accggcgcgc ggcggcgccg cattgcgatc cccgatcagt tattgtcccc
    10321 gccgtgcggg gcgcgctcca tagggcactg catgaaccgg ccgtaggaca aaggcgcagg
    10381 gtccggcttt tacaggccga ggacgttggc gctcatgccc ggctcgccgg tcgcccagat
    10441 ctcgtcgaat cggtcgacga gcgccttggc gcgctcggga ctgccgatcg acagcgatcc
    10501 atgcggccaa tcgatgtggt gccgatgcac gaagtgcagg tcgtcgacga tcacgagcgg
    10561 gtccatcgcc gcacgcgccg ccgcccccgt gcgatacacc ttcatcgcgt gcgcgtggcg
    10621 cacgagcagc gcgcaaagcc gcgcaccgga cgattcgatc cagcgcgtgt cgtgcacgat
    10681 gatgtcgagg cgcgcgcccg gcatgcgccg caggaacgac gacaatgcat cgcagcgatc
    10741 ggccgtattc cagcggccgc ccgacaggtc ggtgtcgaaa atcttgatgt gccgttgtgc
    10801 gagcgcgatc acttcgtcgt gcgcgcgatc gaggtcggca agcgaatcga gccgcgcttc
    10861 gcgcgggagc ccggggggcg gcggaagcgg cgtgtcaggc ggtgtcgacg aggaagccat
    10921 ggcgatgcca atcgtaaagg atggcgacga gctccggcga aaggcgcgca aagacgcgtg
    10981 gcggaagcgt gcgccgatcg gcgagcgttc gcaaatcgtc ggcgcccgag gcgggtagcg
    11041 gcagctcgtc gccattgatg taacaccgcg cgctgtcgta cagcagttgc gtgcgccggt
    11101 cgagtcgcac gcccacgcgc gccatccggc gcgcgaatcg cgcacgcgag cagtgcgcgg
    11161 ccgcggtgaa aacgacttcg ggcttcggct cggtgaggta gcggccgaga aagcgcgcga
    11221 tgtcgtcgtc attccagcgg atcgcagcaa gggcgcgtgc gacacgccgc tgcatcgtgc
    11281 gctcgattcg cgcgggcgct ttcgccggac gcaaaccggg atcggcgtag cggccggaca
    11341 cgtcgagcgt gtcgcgcaga tggtcgagaa aggcctcggc gagctcctgg tgcgcgggcg
    11401 cgcgaaagcc gatcgaatac gtcatgcatt cgtcgagcgc cacgccgtcg tgcgcatgat
    11461 cgggcggcag gtacagcatg tcgcccggtg ccagcgtcgc tgcgtgctcc ggcgcgaagc
    11521 gcgcgaggat gcgcagcggc gcgttctcca cgagccgcag gtcctgctgg cgtccgtagc
    11581 gccagcgcct gcggcccgac gcctgcagca ggaatacgtc gtacgagtcg taatgcgggc
    11641 ccacgccccc gcccggtacc gcgtaactca ccatcaggtc gtcgagacgc acgtagggaa
    11701 tgaacgcgaa gcggcgcagc agcgcgtcgg cgccgtcgtg ctgcaagttc acgccttgca
    11761 ccagcagcgt ccagttgcgc gccggcagcc gctcgagctg cgtgcgtttg aacgggccgt
    11821 cgtgctgcgt gtagcgccgg cccgctcgct gcacgaggcg cgattgcaca tcgtcacgcg
    11881 ccgcgagcgc gaagagttcg gcgcgcgtca gcagcgccgg aaatccgggc agcgccgctt
    11941 cgacgagcag cggcttcttg tgccagtaat cgcgcaggaa tgccgccggc gcatgcccgc
    12001 ccagcagttg gcgcagtgca cgatgcatgg tggcgagtat agcggcggcg gcgctcggcg
    12061 cggaccgcgg cgcgggcgga accgcggcgt acaatgcctg tccaccgagc gggtgcgttg
    12121 catgctcgca gcgcacgggc tcactccttt cttcccctgt catccatgaa cgtcttcgcc
    12181 aacacggtcg tcacgctttc gttccagctc tacgacgcgg gcaacaagct gatcgaggaa
    12241 accgacgagc ccatcgccta cctgcacggc ggtcactccg gcatttttcc gaaggtcgag
    12301 gcggcgctcg atcacaagga ggttggtgac agcgtgtcgg tcacactcga gcccgacgat
    12361 gcgttcggtg actacgacgc ggggctcatg cgcatcgagt ccgtcgagca gcttccgccc
    12421 gacatcgaag tgggcggcca tctcgtcgcc gagcaggacg gcaacgaaat ggtgtggcgg
    12481 atcaccgaca ttgcagaggg caaggcggtg ctcgacggca atcacgagct cgccggccag
    12541 cgcttgcgtt tcgactgcaa ggtgctcgat atccgtcctg cgacgcagga ggaaatcacg
    12601 cacgggcacg tgcatggtgc gcacgggcat catcactgac ccatgcgctg acgcgcaacg
    12661 atcacttttc agccatgatc cgctaccaca atcatccgtc gggccgtcac ttcctgcaga
    12721 tccccgggcc caccaacgtt cccgatcgcg tgctgcgcgc gatcgaccgt ccgacgatcg
    12781 accatcgcgg gccggagttt gcgcaactcg gtctgtcgat tctcggacgc atcggctcga
    12841 tcttccagac gcgcgagccg gtggtcatct atgcggcctc cgggacgggc gcgtgggaag
    12901 cggcgctcgt caacacgctg tcgccgggcg acgtcgtgct catggccgaa accggccact
    12961 tcgcattgtt gtggaagcgg ctggccgagc ggctcggatt gcagcctgaa ttccttcccg
    13021 gcgactggcg gcatggtgcc gaccccggcg ccatccacgc acgcctcgcc gacgatcaca
    13081 cgcacgcgat cagggccgtc tgcatcgtgc ataacgagac gtcgaccggt gtggtgagca
    13141 acatcgggcg cgtgcgcgag gcgatcgatc gcgccggcca tccggcgctg cttctcgtcg
    13201 acaccatttc gtcgcttgga tcgatcgact atcgtcacga tgaatggggc gtcgacgtca
    13261 ccgtggccgg ctcgcagaaa ggattgatgc tgccgccggg cctcgcgttc aacgcggtga
    13321 gcgagaaggc gcgcgcggcg tcgaagcagg cgaaattgcc gcgggcgtac tgggcgtggg
    13381 acgaaatcat cgccgccaac cgcaacggct attggccgac gacgccggcc accaacctgt
    13441 tgtacgggct cgacgcggcg ctcgaccagc ttctcgacga aggcttgccg aacgtattcg
    13501 cgcgtcatgc gcgtcacggc gaagcgacgc gcgcggcggc acgtgcgtgg gggctcgaaa
    13561 ttctgtgcgc gaaccccgac gagtacagca attcgctgac cgccgtgctg atgcccgagg
    13621 gccacgatgc cgatgcgttt cgccgtgtcg tgctcgagcg cttcgacatg tcgctcggta
    13681 ccggactttc gaagcttgcc gggcgcgtgt tccgcatcgg gcacctcggt gatttcaacg
    13741 acctcacgct gatgggcacg ctggcgggcg tcgagatggg cttgcgtgtc gcgggtgttc
    13801 cgcaccgcgc gggcggggtg gacgcggcga tggcgatcct cggcggcaat cgcgcaccgg
    13861 tgggtccgaa ggcgtcgcgc gcggtcgagc tcgcttgacg catcgacgcc gccgatgacg
    13921 gccgcgccgc tgccggagta cctcgcgccg ctccgcgatg cgctggtcga tgcgcggcat
    13981 gggcgcatcg gcgtggcggc cgcggatttg ccggtgccga ggtgtgacga ggatgcgtac
    14041 gcggtccagg aatcggtcgc cgatgcgctc ggctggtttg ccgcggcacc ggcccgcgcg
    14101 tggaaggtcg gcgctgcaag ccgcgccgcg ttgccgaacg ccgcgccgtt gccgtcgcag
    14161 cacgtcgtcg acagtccggc gacatttccc gcgggcgcat tcgatcggat gctgatcgag
    14221 ggcgagattg cgtttcgcct gcgcgcgccg atcgacggcg acgacggcgc cagcgtcatc
    14281 tcgtcgatcg gcgcgtggct cgtgaccatc gaagtcgtcg atccgcgcta cgccgattgg
    14341 gataccgcga gcccgatgca gaagcttgcc gatcaagggc tgcacggggc gctggtcgtc
    14401 ggcagcggca ccgcgttgcc ctcggcgatc gactggactt cactcgttgc gcgcgtgcgg
    14461 cgcaatggcg ggatcgtgcg tgaaacgcgt ggcgggcatc cactcggcaa cctgctgttc
    14521 ctgctgccgt ggctggcgcg gcatgcggcg gcgcgcggca cgccgctttg cgccggcgac
    14581 gtggtcaccg cgggcacgtg gaccggcgtg ttcgaagcgt ttccgggtga gacgatcgac
    14641 gtcgaattcg acggcgtcgg tcgcgcgagt gcgcgcttcg attgatgcgg gaccgcggac
    14701 ggacgcgcta gcgcgtcacg gcgcgcgcat tgcgcgcacc acccagccgt tgacctctgc
    14761 gcccgcctcg cgccgcagca ccgcgggcgc gacatcgagc gcgcggaggt tcgcagcggc
    14821 gaggagacgc tcgacgtagc ggcgcgcatg gctgtaccgg ccgtgcggat tgatccggta
    14881 cgtgtcgatg tcgtcggcac gctccacggt gaagccgaac catcctccgc cgcgcagcgc
    14941 cgatgccgcg gcatcgatgg ccggcgcgag atcgccgaaa tagcacagcg tatcggcgca
    15001 ggcgatcacg tcgaagcgcg ccgtcgcgcc gcgcaggaag tcgaccagct cgccttcgac
    15061 gagcacgtcg tacacgccgc gcacgcgcgc cttgcgcagc attccgggcg aca
//
LOCUS       DASSVX010000228         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_776254, whole genome shotgun sequence.
ACCESSION   DASSVX010000228 DASSVX010000000
VERSION     DASSVX010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776254"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VFO53_09930"
     CDS             complement(<1..662)
                     /locus_tag="VFO53_09930"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET8876633.1"
                     /translation="MSIAPLYSLEFFPPRTPEGVEKLRAARREFMALKPAFCSVTFGA
                     GGSTREGTLATVLEIRSEGMDGAPHVSCIGSTRDGIREVLAKYREHGIRHLVALRGDL
                     PSGSPDVGDFRYANELVRFIREETGDWFHIDVAAYPEVHPQARNPEDDLASFQRKIDA
                     GADSAITQYFFNTDAYWHFVDECGRRGVDVPIVPGIMPIGSFAKLARFSDACGAEIPR
                     WIR"
     gene            complement(662..1021)
                     /locus_tag="VFO53_09935"
     CDS             complement(662..1021)
                     /locus_tag="VFO53_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET8876634.1"
                     /translation="MRLIVTWFLNALALFVLPYILPSIHVASYRSALVAALVLGFINT
                     LIRPILVLLTLPVTLLTLGLFIFVINGLLFWFVGSFITGFTVDGFWSGVFGAIVYSIV
                     SWLLASLIRTRSAGEVS"
     gene            complement(1040..1963)
                     /locus_tag="VFO53_09940"
     CDS             complement(1040..1963)
                     /locus_tag="VFO53_09940"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HET8876635.1"
                     /translation="MRIAIFGTGGVGGYFGAKLARGGNDVAFVARGAHLAAMRANGLR
                     VESAGGDIVVPKVVATDDPSSLAPVDAVMFCVKLWDVEEAARHMAPLLAPGGVVIPFQ
                     NGIDSPGILRRVVGDAHVLGGVAYIAAAIGAPGVIVHTGSMARLRIGAFDARLTDRAR
                     AFVHDCTAAGIAAEFADDITRAIWEKFVFLGALSGLTCLARQPLGVVRGDAELRATFE
                     AAVRETIAVATTKGVEFPQDFPDRQLRALDGLPAEMRSSMLGDLVAGHRLEAPWLCGR
                     VAALAAEAGIAAPVNATIYAGLKPYVDGAPH"
     gene            complement(1998..>2275)
                     /locus_tag="VFO53_09945"
     CDS             complement(1998..>2275)
                     /locus_tag="VFO53_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET8876636.1"
                     /translation="NLGCGTGHPSYVMSSSFANQVMAQIELWTDAKENGGKYPVGVYV
                     LPKHLDEKVARLQLSKLGAKLTELTDKQARYLGVDLAGPYKPDSYRY"
BASE COUNT          428 a          760 c          738 g          349 t
ORIGIN      
        1 cggatccagc gcgggatctc ggcgccgcag gcgtccgaga agcgcgccag tttcgcgaag
       61 ctgccgatgg gcatgatgcc cggcacgatg ggaacgtcga cgccgcggcg cccgcattca
      121 tccacgaagt gccagtacgc atcggtgttg aagaagtact gcgtgatcgc cgaatcggcg
      181 cccgcatcga tcttgcgctg gaagctcgcc aggtcgtcct cgggattgcg cgcctgtgga
      241 tgcacttcgg gatacgcggc gacgtcgatg tggaaccagt cgcccgtttc ttcgcgaatg
      301 aagcgcacga gctcgttcgc atagcggaaa tcgccgacgt cgggactgcc cgaaggcagg
      361 tcgccacgca aagcgaccag atgacggatg ccatgctcgc gatacttggc cagcacctca
      421 cggatgccgt cgcgcgtcga gccgatgcac gagacgtgcg gtgcaccgtc catcccctcg
      481 ctgcggatct cgagcacggt ggcgagcgtg ccttcgcgcg tcgaaccgcc ggcgccgaac
      541 gtcaccgagc agaacgcggg tttcagcgcc atgaattcgc gccgcgccgc acgcagcttt
      601 tcgacgcctt cgggcgttcg cggagggaag aattcgagac tgtagagcgg cgcgatgctc
      661 atcaagatac ttcgcccgcg gaacgcgttc ggatcagcga tgcgagcagc cacgagacga
      721 tgctgtagac gatcgcgccg aacacgcccg accagaagcc gtccacggtg aatccggtaa
      781 tgaacgagcc cacgaaccag aacagcagcc cgttgatcac gaagatgaaa aggccgagcg
      841 tcagcagcgt gaccggcagc gtgagcagca cgagaatcgg ccggatcagc gtgttgatga
      901 acccgagcac gagcgccgct acgagcgcgc tgcgataact cgccacgtgg atcgacggca
      961 ggatgtaggg cagcacgaac aacgcaaggg cgttgagaaa ccaggtgacg atcaggcgca
     1021 gcatcgctcg ttggccttgt cagtgagggg cgccgtcgac atacggcttc agcccggcgt
     1081 agatcgtcgc gttgaccggt gcggcaatgc ccgcttccgc cgcaagagcg gcgacgcgtc
     1141 cgcatagcca cggtgcttcc aggcggtgtc ccgccaccag gtcgccgagc atcgacgagc
     1201 gcatttccgc cggcagccca tcgagggcac gcaactgccg gtctggaaaa tcttgcggga
     1261 attcgacgcc tttcgtcgtc gcgacggcga tggtttcacg caccgccgct tcgaaggtcg
     1321 cacgcagttc ggcgtcgccg cgaacgacgc cgagaggttg ccgcgcaaga caggtaagcc
     1381 cggacagcgc accgaggaac acgaatttct cccagatcgc gcgcgtgatg tcgtcggcga
     1441 attccgcggc gatgccggcc gccgtgcagt cgtgcacgaa cgcccgcgcg cggtccgtga
     1501 gccgagcatc gaatgcgccg atgcgaagcc gcgccatcga gccggtgtgc acgatcacgc
     1561 ccggcgcgcc gatcgcggcc gcaatgtacg caacgccgcc cagcacgtgc gcgtcaccca
     1621 cgacgcgccg caatatcccg ggactgtcga tgccgttctg gaacggaatc acgaccccgc
     1681 cgggcgcgag gagcggcgcc atgtgccgtg ccgcctcctc gacatcccac agcttgacgc
     1741 agaacatcac ggcgtcgacg ggcgccagcg acgatgggtc gtcggtggcg acgaccttcg
     1801 gcaccacgat atcgccgccg gcgctctcga cgcgcaaccc gttcgcccgc atcgcggcga
     1861 gatgagcgcc acgcgcgacg aacgcgacgt cgttgccacc tcgcgccagc ttggcgccga
     1921 aatagccgcc gacaccgccg gtgccgaata tcgcgatccg catgtcagcg ccctttcacg
     1981 gcaggtacca acctggccta gtagcggtag ctgtcgggct tgtacggtcc cgcgagatcg
     2041 acgccgaggt agcgcgcctg cttgtcggtg agctcggtga gtttcgcgcc gagcttcgac
     2101 aattgcagcc gcgcgacctt ctcgtcgagg tgcttcggca gtacgtagac gccgacagga
     2161 tacttgccgc cgttttcctt ggcgtcggtc cagagctcga tctgcgccat cacctggttc
     2221 gcgaacgacg agctcatcac gtacgacgga tgacccgtcc cgcaaccgag gttca
//
LOCUS       DASSVX010000229        25767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_276450, whole genome shotgun sequence.
ACCESSION   DASSVX010000229 DASSVX010000000
VERSION     DASSVX010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 25767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25767
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276450"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..2118)
                     /locus_tag="VFO53_09950"
     CDS             complement(151..2118)
                     /locus_tag="VFO53_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HET8876637.1"
                     /translation="MPRAVWIILGAVGGLVALLLIGVAIAVATVDPGRFVAPLAARVK
                     AETGRDLTVQGPVDITLSLEPKIVLPGVAFGNAPWSKTREMLTAKRIEAQIALIPLLS
                     RRFEVVEFTLVEPVITLETDASGRGNWEFENANPTSTAAPGGGAAGAAAIGIGNLEIR
                     NGTLNYRNGATGKVTIASIERMTLRARDMQAPVAVDFRGKVDEIPVALAGDVGAPDKW
                     LHQQWPVPIALKGDIDGNAVKLDTKIARAGTTTSLDELNAAFGAIGAKGSIKVINDGS
                     RTRYAVDLTIPSLSLKDLPAKAEVKTDKSANSPPAQGATPAPAAAGAWVIPDTPLPIA
                     PFVALNTEGSIAVGEVVLRDGTRVSQVGTHFTSRDASLDAKFTAGTILGGSMHGEVQF
                     DGRNAQAPGVHLLLDAQDLDLAALAAVAGIKREIHGGRVRAGIDIRGQGATPHRIAGT
                     MSGTISVVSGPATLGRSAVQGESALAQIAGALDPLHGVDTATDLRCAVFRLPLSNGVA
                     HVDHSIAIETGKIAASASGTLNFRDETLDLTVQPQIREGVKIDLSQFASLVRIRGRFD
                     KPAVAIDAAQSAKTIAEMAALGATGGGLAVIGKALISSTSGTSAPCEVAMTGKAPRES
                     APLSSSRKAPPRPDLPLPKDLGKALGDLLRR"
     gene            complement(2165..3031)
                     /locus_tag="VFO53_09955"
     CDS             complement(2165..3031)
                     /locus_tag="VFO53_09955"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8876638.1"
                     /translation="MNASDHRLPFAVRPLTPNLGAEILEVTLADAMTDDIFAGIHRAF
                     LQHQVLLFPAQDVPPERQVALARRFGEVQIHVMNQYHADGYPELYRLSNLDAEGKPNG
                     RHPDKGTLAWHTDGSWRRRSGQATIIYGEVMPRHGGETHFCDMYGAYERLDAAWKRRI
                     ANLRAVHNLDFSRNRRHGEDPMTDEQRRATPPVDHPVVRTHPQTRRKCLFLGDHAESI
                     VGMPYEEGRALIEELNALAPHPDLTYEHHWQPGELLVWDNRCTMHRATPYDPVTEGRV
                     IRRCTVLGDIPE"
     gene            complement(3051..4388)
                     /locus_tag="VFO53_09960"
     CDS             complement(3051..4388)
                     /locus_tag="VFO53_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET8876639.1"
                     /translation="MTHGAILLLMTGLFLLFGYMGVPVPFALIAGVLAATAFTQISMQ
                     SMVGQLFHGIDSEALLAVPFFLLVGELMASSDVAHRMIRLSQTLVGHLRGGLAQVVTL
                     FSMFFAGISGSSTADVAVLSRTIARDMDREGYDRAFTAALIASASTMANLIPPSIMAV
                     VYGATGNVSIGGLFLAGVVPGVLIGIGLMIYSYFRGPIGIRKARAPLTEVVIAARGSA
                     LPLVIPVIIMGGILSGFFTPTEAGMVAAVYIIVVLIPLLRPAHVKELPRDFMYAGLLY
                     SLPLAAVAGASAFGWMVAYLRGPDIVATYIGGIAGTDGTRIMFLLVALFVIVGDFIDA
                     VPAIIIFMPIIIKLAALGDINPLHMGVVIITTLVFGLITPPYGLSLLVASKYVGVGFG
                     KAVLRSLPLYVVFLATIAFTVVFPDIVLWLPKHFLPESVGCFKSPTGVGYICP"
     gene            complement(4385..4936)
                     /locus_tag="VFO53_09965"
     CDS             complement(4385..4936)
                     /locus_tag="VFO53_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HET8876640.1"
                     /translation="MADFRALILERHRHLKWKALDPLEALLMILCGTCIAMFTLTVLC
                     DVATRLIGAPWLWLQQVTTAFFAWGVFIGMAAATRRNDHFYLTEITKRMTGRSRNSIE
                     IMNRLIVLVVSVLIVVCGWQNAVLDLGSFRMPSLIPLTVYTAIVPVAGALIALFSVEQ
                     LVNGWRHGFAGPEDRDDTREAVL"
     gene            complement(5022..6029)
                     /locus_tag="VFO53_09970"
     CDS             complement(5022..6029)
                     /locus_tag="VFO53_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HET8876641.1"
                     /translation="MQRFIGNKVLRALVLFAAALSASVASGQAKREFSFAYDQPTTTA
                     YGIAANIFDAKLKELSGGTMSINQFPGAQLGQEPQMLQKMRAGDIDFVITSTANASTV
                     APQAGVFSLHFIFRDQDHLRKVLADPAIAQEFRDMVKDSVQGAQVLGLLTMGMRNMYS
                     KKPITSMADIKGEKVRVQATKTEDTLFPAYGAQTVHMPFGDVYTSLQTGVVNVAENGV
                     NVYMANKHYEVAPILSMTEHEANNNCLWVSDKTWNSLTPQQQQWVQAAATEVSRTEPA
                     KALQLEKDSAAKLSKMGVKIVEGVDKAGFMKAAAPVQDQLAAELGPHAVKLLKMVRDE
                     K"
     gene            6264..7133
                     /locus_tag="VFO53_09975"
     CDS             6264..7133
                     /locus_tag="VFO53_09975"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8876642.1"
                     /translation="MNDRACLAPDPAVHPLRFRVPAGAWDTHIHAIGPVERFPLAALR
                     SYTPAQVPIDAYVALMDRLGITHAVLVQPSIYGSDNRAMLDALSRYPGRFRGVAVVDP
                     AIDDRALRALGAAGVRGVRANLLNPGGISLADACALAGRLAALGWHLQLQIDASTFDS
                     FDALETLPVAVVIDHMGFLPADLGPLNAGFRRLLQLVESGRCWVKLSAPYRLVDWQRR
                     GYGAVAPLARALVRANPQRVLWGSDWPHTDLRSAMPNDGDLLDLVGAWVDDAAIRNAI
                     LVDNPAALYGGRF"
     gene            complement(7115..7939)
                     /locus_tag="VFO53_09980"
     CDS             complement(7115..7939)
                     /locus_tag="VFO53_09980"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HET8876643.1"
                     /translation="MHAQRFVTEAALVADLAAARYRLLGEIHDDPAHHAIRARLLSEI
                     AQRGAHPAVVMEQFDLNRDEALRAAQNAGGDAERVATAGELDRTGWRWPLHEPIVAAA
                     LAARLPLRAGNVSRRVLTGDLDAMLEKHPALRSRLHAAPWTDVQARSLRADIVESHCG
                     MLPDSVVPRLVLAQRIRDAAMAQALAGDATDDGAILIAGNEHVRADRGVPVYLHAPGF
                     TDAGVRSVSVGFVEAGARDERAAGFPRSAVAQYPGFDYLWFTPSVARDDPCQKRPP"
     gene            complement(8022..9077)
                     /locus_tag="VFO53_09985"
     CDS             complement(8022..9077)
                     /locus_tag="VFO53_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET8876644.1"
                     /translation="MTYLAKPKIHHPGLPKNSLGYTRRDYEGAISTLCAGCGHDSISA
                     AIIQAFYELDIAPHRVAKLSGIGCSSKTPTYFLGNSHGFNSVHGRMPSVLTGAALANR
                     DLIYLGVSGDGDSASIGLGQFAHVLRRGVDMVYIVENNGVYGLTKGQFSATADKGSKS
                     KRGAVNSDEAIDLVLLALALGATFVARGFSGDKAQLVPLIKAAVAHRGAAFIDIVSPC
                     VAFNNHSGSTKSYDYVREHNEAVNRLDFMPARAPIETDYPEGGVREVQLHDGSTVRLA
                     KLDASYDPSDKVGAMAYLESRRARGEVVTGLLYVDEQSDDMHAALSTVGVPLNALGDT
                     DLVPGNAALGGVNASLR"
     gene            complement(9074..10933)
                     /locus_tag="VFO53_09990"
     CDS             complement(9074..10933)
                     /locus_tag="VFO53_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET8876645.1"
                     /translation="MRPSPLTRVNDFVVKFANVNGSGSASANGLFARAILRMGVPVAA
                     RNIFPSNIQGLPTWYEVRVVEAGWRGRRGGVDLMVAMNPQTWDKDVAEIEAGGYLFYD
                     STKSLPPSKFRDDVALLGVPLTAICNAAYSDVRQRQLFKNIVYLGALSALLGIDAAEI
                     ERLLAEQYRGKEALLESNRKALAMGRDHALAHFDCPIGLSVARRDAVGERIFIDGNSA
                     AALGAVYGGATVAAWYPITPSSSLAESFAKWCRKFRTDPVTGDHRYAIVQAEDELASI
                     GMVIGAAWNGARAFTATSGPGVSLMQEFLGLAYFAEIPAVLFDVQRGSPSTGMPTRTQ
                     QADLLSCAYASHGDTKHVLLMPEDPRECFEFAALAFDLADRLQTPVFVMLDLDIGMQD
                     WLCEPLAWDDRRTMDRGKVMTAADLDAGRDFGRYLDVDGDGIAYRTYPGTHAAKGSYF
                     TRGTTKDRYARYTEEGPAYVDNMQRLLRKFETAKALLPKPVITRAEQATTAGVVWFGS
                     TGAAMAEALAELAHQDVHLDRMRLRAFPFDDTVAEFIAAHEHVFVVEQNRDAQLKMLL
                     VNECGIDPAKLTSVLHYDGTPITERFITREIGEKARLYNVKPLTIRRTGGRAA"
     gene            complement(10935..12803)
                     /locus_tag="VFO53_09995"
     CDS             complement(10935..12803)
                     /locus_tag="VFO53_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8876646.1"
                     /translation="MKPTDIKHPDYFHKVVDCQWACPAHTPVPEYIRLIAAGRYGDAY
                     LVNWKSNVFPGILGRTCDRPCEPACRRVRVEAGSAEHRRTDAKPEPVAICRLKRVAAD
                     NKEDIRPRLPGPPAQRNGKRIALIGAGPASLTVARDLAPLGYECVVFDADAKAGGMMR
                     TQIPKFRLPDRVIDEEVSYVLDEGIDFRAGVRIESLARLLAPEATERWDAVFVGSGAP
                     RGRDLDIPGRREAAAGIHIGIDWLSSVSFGHITSIGRRVVVLGGGNTAMDCCRSSRRL
                     GGEEVHVVVRSGFDEMKASPWEKEDAMHEGIPIHNYLVPLEFTHDGGRLTGVTFEKVA
                     AQRDARGRRQLVATGEPPVHFPCDDVLVAIGQENAFPWIERDIGIAFDEWSMPIVDRT
                     TMASTRSGVYFGGDAAFGPKNIIWAVAHGHDAAISIDLQCRGEPLSDRPPPHVNLMSQ
                     KMGIHEWSYDNAIHSDRRYRVPLKDVTIALKDIRTEVELGFDAKLAYAEAQRCLNCDV
                     QTVFADRLCIECDACVDICPMDCITFTTNGDEADLRSRLMAPALNVDQALYVSTPLKT
                     GRIMAKDEDVCLHCGLCAERCPTGAWDMQKFLLDMAHADDRAHAGRKRREPALAAD"
     gene            13048..14103
                     /gene="selD"
                     /locus_tag="VFO53_10000"
     CDS             13048..14103
                     /gene="selD"
                     /locus_tag="VFO53_10000"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HET8876647.1"
                     /translation="MNAPERPSVRLTRFSHGGGCGCKIAPGVLQQILGRVDRTLIPDA
                     LLVGIETADDAAVYRLNERQAIVATTDFFMPIVDDPFDFGAIAATNAISDVYAMGATP
                     LFALAIVGMPIDALPLDVIGRILEGGQSVCARAGIPVAGGHTIDAVEPIYGLVAIGVV
                     DPRALKRNAGARAGDAIVLGKPLGVGIYSAALKCDSLSADGYAALVATATALNTPGIA
                     LARLDDVHALTDVTGFGLLGHLLEMCRGANLAATIEFERVPLLPQAMSLASAGFVTGA
                     SRRNFDAFGNDIVSGERIGGRERALLTDPQTSGGLLVACAPHALDDVLDIFRRDGFAS
                     AAPIGRFGAGVPRITIT"
     gene            14117..15382
                     /gene="egtB"
                     /locus_tag="VFO53_10005"
     CDS             14117..15382
                     /gene="egtB"
                     /locus_tag="VFO53_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HET8876648.1"
                     /translation="MPMPGATALAVPSLPLLRRYRNVRARSATLVAPLSPEDCALQSM
                     PDASPAKWHLAHTTWFFETFVVAPRIAGYRAFDPAFRVLFNSYYHAVGDRHPRPERGM
                     LSRPPLDAVLAYRASVDEAMYECCERARNDAALASLIELGLAHEEQHQELILTDVLHL
                     LSRNPLAPAYDPEGPAPVHRARDMGWIRYRAGLANIGHAGDGFAFDNEMPRHRVWLES
                     FALASRPVTNAEYAAFIDDAGYRRPELWLALGWDTVTAGNWQAPLYWIRDDDRWQSFT
                     LQGVADLDPHAPVAHISFFEADAYARWAGARLPTEYEWEAVAARGTDARTGDPWAGSL
                     GTTVTLACGDVWEWTSSSYAPYPGFRAAPGAVGEYNGKFMCNQYVLRGSSSATPPGHA
                     RITYRNFFAPDARWQWSGVRLARDHAPAP"
     gene            complement(15364..16377)
                     /gene="egtD"
                     /locus_tag="VFO53_10010"
     CDS             complement(15364..16377)
                     /gene="egtD"
                     /locus_tag="VFO53_10010"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET8876649.1"
                     /translation="MQAGAHRLHATPDAALMPALEQRLCDRRVHDAGAERRALVGGLL
                     STPASIAPKYFYDARGSALFAEICRLPEYYPTRTEAAIFERHRDDIAAAAGTGREFVD
                     LGAGDCEKAARWLPFLQPWRYVGVDIAPEALAPALARIANAHPRIEVRGLLTDFAHGL
                     DLFRDLGPGAVTFFYPGSSIGNFTPVEAQHFLQAIRRHARGAASGLLIGADTKKDAAR
                     LTAAYDDAAGVTAAFNRNALAHVNRILGTRFDPAAFAHVAFYNERDSRVEMHLEARTR
                     QTVVVDGIARTFAAGERIHTENSYKYAPPEFVAMLERAGLRDVRCWQDDAGDFAVYYG
                     AGA"
     gene            16522..17688
                     /locus_tag="VFO53_10015"
     CDS             16522..17688
                     /locus_tag="VFO53_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876650.1"
                     /translation="MTVLAALAFTAGAHAQIDPVRRDLVEFGYGQPLAGHAPVGAYVF
                     YYLSRPQFGDADHALRLAVSPVYLDGEWAWRNALGAHTDLAIGVSGGGFASDYNEIRA
                     GHWLRDQSFTGHDGGVDVSAYHLFDPGARVPVYGILRGDFRYVAFERDSENPPNFILP
                     DNQPVASVRAGLRAGGIEPVLAPDVAGELSAWYETQVRLNAGTYGFDGDRRVQRTVQR
                     LWGRGLLVYTLPQSRHRVAFSLVGGTSVHPDRLSAWHIGGVLPLDSEFPLVLPGYYEG
                     EFSARNFVLAGADYAVPLDRGEHWQLGVGAAVARIGFTPGLEGPRAWNSGMSASLGYA
                     PDTKAWKASLVYGRALDAVRNGHRGADSVTLLMEFDLERMGYLPADASRPRRPD"
     gene            complement(17691..18887)
                     /locus_tag="VFO53_10020"
     CDS             complement(17691..18887)
                     /locus_tag="VFO53_10020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8876651.1"
                     /translation="MRSDRVAPVALRPLVTALGIGQIVSWGTLFYTIAVLGPPMAREL
                     RVSDVMLYGSFTAGLFLSGIASPWVGSRIDRRGGRAVLAGGSALGALACALLATSVNG
                     AMMLAGWLVAGVAMAACLYDPAFATLFRVSGASYRRAVTALTLFGGFASTVFWPLSQY
                     LLETHGWRVAFGVHAALNALVCVPLHLAFVPSSAHRSVAPASAGTAETALTRRGTFAW
                     LAAALSVAAFLASAVSAHLVVLLTSGGLAARDAVLVGALIGPMQVAGRVMEFAFSNRW
                     SPLTVGTLAFTLLASALIVLCLVRGVWIVALAFALLYGWSNGVMTIVRGTVPGVLFGA
                     RDYGALLGRLAQPQFILKAFAPVAVTLLFALDDTRRLALYALAFAAAGALIAYRVAVR
                     GIATRR"
     gene            complement(18877..20142)
                     /locus_tag="VFO53_10025"
     CDS             complement(18877..20142)
                     /locus_tag="VFO53_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET8876652.1"
                     /translation="MDDDRIIGWLAEHRGEAEAFLAALVREPSDNPPGDCAPHAAKAA
                     AMLETMGLDVERHRVPDDEVRAHGMVSCTNLIVRSTFGRGAGPVIAMNAHGDVVPPGS
                     HWTTDPYGAAVRDGVMYGRGVAVSKSDFATYAFALRALQHAAEHGRAFDGTVELHFTY
                     DEEAGGEIGPAWLLAHGLTRPDYTISAGFAYAITTAHNGCLHLEVEVTGMSGHAAEPE
                     RGVDALEAATGILTDLYALRGTYTGIRSTIPGITHPTLVVGLIDGGINTNVVPDRVRF
                     RIDRRIIPEENPGLVEATLRDAIDDFAQQWPGAGVRVRRILLARPFVPIAGQERLVGA
                     LQRAARRCLGEVLPLQGVPIYTDARHYTAAGVPTVLYGAGPRTLAEANGHRADERLRL
                     DDLARASGVVAVALADLLTPGERRSTHAQ"
     gene            complement(20164..20937)
                     /locus_tag="VFO53_10030"
     CDS             complement(20164..20937)
                     /locus_tag="VFO53_10030"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET8876653.1"
                     /translation="MPEHLLIETRERVGLIRINRPQRMNALNNQVAREIADALFAYDA
                     DPAIGCTVITGNDKAFAAGADIGAMAEWDYAKVYGDDYITAEWEGIRRVRKPVIAAVA
                     GYALGGGCELAMACDIIIAADNARFGQPEITIGTMPGFGGTQRLPRAAGKSKAMEWCL
                     TGRMFDAQEAERAGLVARVVPLAALEQEAMALAAKIASFSLPVVMKIKEAINRAYESS
                     LTEGLAFERREFHSTFALDDQKEGMRAFVEKRKPAFRHR"
     gene            21080..22438
                     /locus_tag="VFO53_10035"
     CDS             21080..22438
                     /locus_tag="VFO53_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase"
                     /protein_id="HET8876654.1"
                     /translation="MKKVYANATEALSGLLANDITIAAGGFGLCGIPENLIQAVVDSG
                     VKGLTIAGNNAGVDDFGMGLLLKGHQVKKVIASYVGENKEFERQVLAGELQLQLTPQG
                     TLAEKLRAGGAGIPGFYTPTAAGTLLAEGKETKAFDGREYVLEESIRADVSIVKAWKG
                     DRAGNLVYHETARNFNPMIATCGKVTVAEVEELVDVGELDPNGIQTPGIYVDRIFRGT
                     NYQKRIEFRTVAGTAGKKDSPVREMMARRAARELRDGYYVNLGIGIPTLVANFIPQGI
                     SVTLQSENGLLGIGPFPADNEVDPDLINAGKQTITTLPGSSFFSSADSFAMIRGGHID
                     LSILGALEVAQNGDLANWMVPGKMVKGPGGAMDLVSGVKRVIVLMEHTARDGSPKILE
                     RCTLPITGKNVVDLIVTDLCVFEVKPDGEGLVLRELHEGVTLDDVKAKTGCAFEVALE
                     RR"
     gene            22482..23471
                     /locus_tag="VFO53_10040"
     CDS             22482..23471
                     /locus_tag="VFO53_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HET8876655.1"
                     /translation="MRVAILDDYHDTLRTLDAFAKLAGHDVTIFNDHVQDVGRLAARL
                     ADAEALVLIRERTQIRAPLLERLPKLRLISQRSVYPHIDIDACTRLGIVVSSSQHAGT
                     PSYAAAELTWGLVLAAMRDIPQQMNALKAGKWQIGVGHTVRGKTLGVYGYGRIGAVVA
                     GYGKAFGCNVVVWAREASRERARADGYAAADSKAAFFAESDILTLHMRLVDATRGIVT
                     AADLARMKPTALLVNTSRAGLVAPGALVEALRNGRPGMAGVDVYDQEPLVDRNDPLLS
                     LDNVVCTPHIGYVTRDEYEVQFAEIFDQIVSYAAGKPINVVNPGVLDHMRRAS"
     gene            23482..24540
                     /locus_tag="VFO53_10045"
     CDS             23482..24540
                     /locus_tag="VFO53_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876656.1"
                     /translation="MPHLELDGLTKRYGDVTSVDAIDLTVERGEFICLLGPSGCGKTT
                     TLRMLAGFLIPDAGEIRVAGKTLSSSSAVVPPERRNMGMIFQSYAVWPHMTVRENVGY
                     GLRMQSVPATERNGRVDALLDATRLREHADRYPAELSGGQQQRVALARALAPNPGTLL
                     LDEPLSNLDANLRGEMRFEIRRLHDEYRNTSIYVTHDQVEAMTMADRIVVMNAGRIEQ
                     IGTPEDVFDRPHSRFVARFIGGSNLLEGTRVDGRRVDVAGHALDVGHGEFAGRGEPMS
                     VCVKTHDMHIDADGRADGTNVLPGVVRSQAYLGSHRDYIVDVGQPLLIAAPAALNIPA
                     GSSVTVRFRPDRCRGIIR"
     gene            24592..25623
                     /locus_tag="VFO53_10050"
     CDS             24592..25623
                     /locus_tag="VFO53_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8876657.1"
                     /translation="MRITRIAAFAAALFTCAVAMQALAQETTPAMLAAAAKEGKVVWY
                     TAVDVKVAESVAKVFRAEYPQIALEVERSGSERVFQRINQEFQSNIHNVDVVNSSDAS
                     HFLLWKQQKLLAKHVPPDVQRFAAQYKDPDGYYAVWRASLSVMGYNTNLVKPGDAPKG
                     YRDLLDPKWKGKLVKSHPGYSGTSLTGTFALEKVLGWDYFEKLARQGVQQLQSTTAPP
                     KSIASGERAVMVDGNEYNMFIEIQAKSPVKIIYAVEGTPFVTSPTAIFAEAPHPNAAR
                     VLQNFLYTAKVQQLLVNEGGLRSVHPDVKEPADRTPLKDIKLLADDPVAMLPQVAEIK
                     KRYTAIFGN"
BASE COUNT         4241 a         8728 c         8689 g         4109 t
ORIGIN      
        1 gtcccaagcg cgaatcggcc ccggagcgcg aagcgcggag gtagtcccat gagcgcgcgg
       61 ggccgtccca agcgcgaatc ggccccggag cgcgaagcgc ggaggtagtc ccatgagcgc
      121 gcggccgcgc gcccgttcgc cagcggctgg ctagcggcga agcaggtccc cgagcgcctt
      181 gccgaggtcc ttcggcaggg gaagatcggg gcgcggcggc gccttgcgtg acgacgacag
      241 cggcgccgat tcgcgcggcg ccttgccggt catcgcgacc tcgcaaggcg ccgatgttcc
      301 gctcgtcgac gagatcaacg ccttcccgat caccgcgagc ccgccgccgg tggcgccgag
      361 cgcagccatc tccgcgatcg tctttgccga ctgtgcggca tcgatcgcga cggccggctt
      421 gtcgaagcgg ccgcggatgc gcacgaggct tgcgaactgc gacaggtcga tcttcacgcc
      481 ttcccgaatc tggggctgca ccgtcaggtc gagcgtctcg tcgcggaagt tcagcgtgcc
      541 gctcgccgat gccgcgatct tgccggtttc gatcgcgatc gaatggtcga cgtgcgcgac
      601 gccgttgctg agcggcaagc ggaatacggc gcagcgcaga tcggtggccg tatcgacgcc
      661 gtgcagcggg tcgagcgcgc ccgcgatttg cgcgagcgcc gattcgccct ggacggccga
      721 gcggccgagc gtcgcgggtc ccgatacgac gctgatcgtc ccgctcatcg tgcccgcaat
      781 gcgatgcggc gttgcgccct gaccgcgaat gtcgatgccg gcgcgcaccc gcccgccgtg
      841 gatttcgcgc ttgatgcccg ccaccgcggc cagcgccgcg agatcgaggt cctgtgcatc
      901 gagcagcaga tgaacgcccg gcgcctgcgc attgcggccg tcgaactgca cctcgccgtg
      961 catcgaacca ccaaggatcg tgccggcggt gaacttggcg tcgaggctcg cgtcccgcga
     1021 cgtgaagtgc gtgccgacct gcgatacgcg cgtgccgtcg cgcagcacga cctcgcccac
     1081 cgcgatcgat ccctcggtgt tcaacgcgac gaacggcgca attggcagcg gcgtgtcggg
     1141 gatcacccac gcgccggccg ctgcaggcgc cggggtcgcg ccctgggccg gcggggagtt
     1201 cgcgctcttg tccgttttca cttcggcctt tgcaggcaag tccttcagcg acagcgacgg
     1261 gatcgtgagg tcgaccgcgt agcgcgtgcg tgagccgtcg ttgatcacct tgatcgatcc
     1321 cttggcgcca atggcgccga acgccgcatt cagctcgtcg aggctcgtcg tcgtgcccgc
     1381 gcgcgcgatt ttcgtgtcga gcttgacggc attgccgtcg atgtcgccct tgagcgcgat
     1441 cggaaccggc cattgctggt gcagccattt gtcgggcgcg ccgacgtcgc ccgcgagcgc
     1501 caccggaatt tcgtcgacct tgccgcgaaa atcgacggcc accggcgcct gcatgtcccg
     1561 cgcgcgcagc gtcatgcgtt cgatcgaggc gatcgtgacc ttgccggtcg cgccgttgcg
     1621 atagttcagc gtgccgttgc ggatctcgag gttgccgatg ccgatggcgg ccgcacccgc
     1681 cgcaccgccg cccggcgcag ccgtggaagt cgggttcgcg ttttcgaatt cccagttgcc
     1741 gcgaccgctc gcgtcggttt cgagcgtgat caccggctcg accagcgtga actcgacgac
     1801 ctcgaagcgg cgcgacaaga gcgggatcag cgcgatctgc gcctcgatgc gcttcgccgt
     1861 cagcatctcg cgcgttttcg accacggcgc attgccgaag gcgacgcccg gcagcacgat
     1921 cttcggctcg agcgacagcg tgatgtcgac cggcccctgc actgtcaggt cgcggccggt
     1981 ttcggccttg acgcgcgccg cgagcggtgc gacgaaccgg ccgggatcga ccgttgcgac
     2041 ggcgattgcg acgccgatca acagcagcgc gacgagcccg ccgacggcgc cgagaatgat
     2101 ccagacggca cgaggcatgc cgcctattct agacctcgcc tcgcgcgacg cgagggatct
     2161 cgacctactc cggaatgtcg cccagcacgg tgcagcggcg gatgacgcgc ccttcggtta
     2221 cgggatcgta gggtgtggcg cgatgcatcg tgcagcggtt gtcccagacg aggagctcgc
     2281 ccggttgcca gtggtgctcg taggtgagat cgggatgcgg cgccaacgca ttgagctcct
     2341 cgatcagcgc gcgcccttct tcgtacggca tgccgacgat cgattcggca tgatcgccga
     2401 ggaacaggca cttgcgcctc gtctgcggat gcgtgcgcac gacggggtga tcgaccggcg
     2461 gcgtcgcgcg ccgctgctcg tcggtcatcg gatcttcgcc atggcgcctg ttgcgcgaaa
     2521 agtcgaggtt gtgcacggcg cgaaggttcg cgatccgccg cttccacgcc gcgtcgagcc
     2581 gctcgtacgc gccatacatg tcgcagaaat gcgtttcgcc gccgtggcgc ggcatcacct
     2641 cgccgtagat gatcgtcgcc tgcccggagc gccggcgcca cgagccgtcc gtgtgccacg
     2701 cgagcgtgcc cttgtcggga tggcgtccgt tcggcttgcc ttccgcgtcg aggttcgaca
     2761 gtcgataaag ctccgggtaa ccgtcggcgt ggtactggtt catcacgtgg atctgcactt
     2821 cgccgaagcg gcgcgccagc gcgacctggc gttcgggcgg cacgtcctgc gcgggaaaga
     2881 gcagcacctg atgctggaga aatgcgcgat gaatgccggc gaagatgtcg tcggtcatcg
     2941 cgtcggcgag cgtgacttcg aggatctccg cgccgaggtt cggtgtcagc ggacgcacag
     3001 cgaacggcaa gcgatggtcg gaggcgttca tggcggcgag caggcaacca tcacggacaa
     3061 atgtaaccga caccggtcgg actcttgaag cagccgaccg actcgggaag aaaatgcttc
     3121 gggagccaaa gcacgatgtc ggggaagacg acggtgaatg cgatcgtggc gaggaacacg
     3181 acgtaaagcg gcagcgagcg taacaccgcc ttcccgaagc cgactccgac gtatttggac
     3241 gcgacgagca gcgacaatcc gtacggcggc gtgatgagcc cgaacaccag tgtagtgatg
     3301 atgacgacgc ccatgtgcag cggattgatg tcgccgagcg ctgcgagctt gatgatgatc
     3361 ggcatgaaga tgatgatcgc gggaaccgca tcgatgaaat cgccgacgat cacgaacagc
     3421 gccaccagca ggaacatgat ccgcgtgccg tcggtgcccg cgatcccgcc gatgtaggtc
     3481 gccacgatgt cgggcccgcg caggtaagcg accatccagc cgaacgccga cgcaccggcc
     3541 accgccgcga gcggcagcga atagagcaat ccggcgtaca tgaagtcgcg cggcaactcc
     3601 tttacgtgcg ccggacgcag cagcgggatc agcacgacga tgatgtacac ggccgccacc
     3661 atccccgctt cggtcggcgt gaagaatccg ctcaggatgc cgcccatgat gatcaccggg
     3721 atcacgagcg gcagcgccga gccgcgcgcg gcgatcacga cctcggtcag cggcgcgcgt
     3781 gccttgcgta tgccgatcgg accccggaag tagctgtaga tcatcaagcc gatcccgatc
     3841 agcacgccgg gcacgacgcc agccaggaac aggcccccga tcgacacgtt gcccgtcgca
     3901 ccgtagacca cggccatgat gctcggcgga atcaggttcg ccatcgtcga cgccgacgcg
     3961 atcagtgcgg ccgtgaacgc gcggtcgtag ccttcgcgat ccatgtcgcg cgcgatcgtg
     4021 cggctcagta cggcgacatc cgcggtcgac gagccggaaa tgccggcgaa gaacatgctg
     4081 aacagcgtca cgacctgcgc gaggccgccg cgaagatgac cgacgagcgt ctgcgacagc
     4141 cggatcatcc gatgcgcgac gtcggacgac gccatcagct cgccgacgag caggaagaag
     4201 ggcacggcga gcagcgcttc ggagtcgatg ccgtggaaga gctgtcccac catcgactgc
     4261 atgctgatct gcgtgaacgc cgtcgccgcc agcacgccgg cgatcagcgc gaacggcacc
     4321 gggacgccca tgtagccgaa cagcaggaaa aggcccgtca tcagcaacag gattgcgccg
     4381 tgcgtcacag caccgcctcg cgcgtgtcgt cgcggtcttc gggccccgcg aacccgtgcc
     4441 gccagccgtt gacgagctgc tcgacgctga agagtgcgat cagcgcgccg gcgacgggca
     4501 cgatcgccgt gtagaccgtc agcggaatca gcgacggcat gcgaaagctg ccgaggtcga
     4561 gcacggcgtt ctgccatccg cacacgacga tcagcaccga caccacgagc acgatcagcc
     4621 ggttcatgat ctcgatcgag ttgcgcgacc gaccggtcat ccgcttggtg atctccgtca
     4681 ggtaaaaatg atcattgcgc cgggtcgccg ccgccatgcc gatgaacacg ccccatgcaa
     4741 aaaacgcggt cgtaacctgc tgcagccaca accacggtgc gccgatcagc cgcgtcgcga
     4801 cgtcgcacag caccgtcagc gtgaacatgg cgatgcaggt gccgcacagg atcatcagca
     4861 gtgcctcgag cggatcgagc gccttccact tgaggtggcg atgccgctcg aggatcagcg
     4921 cgcggaagtc ggccacgtca atcccgcgag cggctcgtgg gcgggcgcag gatgccgggc
     4981 aggcgctgct tcgcgcgcct gcccgatccc atgatcacgt gctacttctc gtcgcgcacc
     5041 atcttgagga gcttcaccgc gtgcgggccg agctcggcgg caagctggtc ctgcaccggc
     5101 gccgcggcct tcatgaagcc cgccttgtcg acgccctcga cgatcttgac gcccatcttc
     5161 gagagcttcg cggccgaatc cttctcgagc tgcaatgcct tcgcgggctc ggtcctgctc
     5221 acctcggtcg ccgccgcctg cacccattgc tgctgctgcg gcgtgagact gttccacgtc
     5281 ttgtcactga cccacaggca attgttgttc gcctcgtgct cggtcatcga caggatcggc
     5341 gccacctcgt agtgcttgtt ggccatgtac acgttcacgc cgttctcggc gacgttgacg
     5401 acgccggtct gcagcgacgt atagacgtcg ccgaacggca tgtgaaccgt ctgcgcacca
     5461 taggccggaa agagcgtgtc ctcggtcttc gtcgcctgga cgcgcacctt ctcgcccttg
     5521 atgtcggcca tcgacgtgat cggcttcttg ctgtacatgt tgcgcatgcc catcgtgagc
     5581 agcccgagca cttgcgcccc ctgcaccgaa tccttcacca tgtcgcggaa ctcctgcgcg
     5641 atggcagggt cggcgagcac cttgcgcaga tgatcctggt cgcgaaagat gaaatgcagc
     5701 gagaacacgc ctgcctgcgg agcgacggtc gacgcatttg ccgtcgacgt gatgacgaag
     5761 tcgatgtcgc ccgcacgcat tttctgcagc atctgcggct cctggccgag ctgcgcgccc
     5821 gggaattggt tgatgctcat cgtgccgccc gaaagctcct tcaacttggc gtcgaagatg
     5881 ttcgcggcga tgccatacgc ggtcgtcgtc ggctggtcgt aggcgaagct gaactcgcgc
     5941 ttcgcctggc cgcttgcaac ggacgccgaa agcgcggcag caaagagtac gagcgcgcgc
     6001 aataccttgt tgccgatgaa cctttgcatg gcaagccctc ctgttgtgcg gcgtgacctc
     6061 gatcgcttcg gatgcgaagc gggaataccg gcatgcctcg gcgtctgttc atcgctggcg
     6121 gcacctccat ccccggccgg cagcgggcac gtggggcggg agtattggag gtttcgcccg
     6181 gcgggtcaaa tgcgcacggc ggccgggcgc ctgccctccg gctcgccgcg gcaggcgtcg
     6241 caagggacgg cggtggcacg ggcatgaacg atcgcgcctg tctcgcaccc gatccggccg
     6301 tgcatccgct gcgcttccgc gttcccgccg gtgcgtggga cacgcacatc cacgcgatcg
     6361 gccccgtcga gcgcttcccg ctcgccgcgc ttcgctcgta tacgccggcg caagtgccga
     6421 tcgacgcgta cgtggcgctg atggatcggc tcggcatcac gcatgcggtg ctcgtgcagc
     6481 cgagcatcta cggcagcgac aatcgcgcga tgctcgacgc gctgtcgcgg tatccggggc
     6541 gctttcgcgg cgttgccgtc gtcgatccgg caatcgacga tcgcgcattg cgggcgctcg
     6601 gcgccgccgg cgtgcgcggc gtgcgcgcga acctgctcaa tcccggcggg atttcgctgg
     6661 ccgacgcgtg cgcgctcgcg ggtcgtctcg ccgcgctcgg gtggcacctg cagctgcaga
     6721 tcgacgcgtc gacgttcgac agcttcgacg cgctcgaaac gcttccggtc gcggtcgtga
     6781 tcgaccacat gggcttcctg ccggcagacc tgggaccgtt gaatgcgggg tttcggcgac
     6841 tattgcaact ggtcgaatcg ggccggtgct gggtcaagct ttcggcgccg tatcgcctcg
     6901 tcgactggca gcgccgcggg tacggcgccg tggcgccact cgcgcgggcg ctcgttcggg
     6961 cgaacccgca gcgcgtgctg tggggcagcg actggccgca taccgatctg cgcagcgcca
     7021 tgcccaacga tggcgacctg ctcgacctgg tcggcgcatg ggtcgacgac gcggcaatcc
     7081 gcaacgcgat cctcgtcgac aatccggcgg cgctctacgg cgggcgcttc tgacagggat
     7141 cgtcgcgcgc aacgctcggc gtgaaccaca gataatcgaa tcctggatac tgcgcgacgg
     7201 ccgatcgtgg gaaaccggcg gcgcgctcgt cacgggcgcc cgcttcgacg aatcccacgc
     7261 tgaccgagcg cacgcctgca tcggtgaatc cgggcgcatg gaggtagacc ggtacgccgc
     7321 ggtcggcgcg cacgtgctcg ttgccggcga tgagaatcgc gccgtcgtcc gtcgcatcgc
     7381 cggcgagcgc ctgcgccatc gcggcgtcgc gtatccgctg cgccaggacg agccgcggga
     7441 cgaccgagtc gggcagcatg ccgcagtggc tttcgacgat gtcggcccgg aggctacgcg
     7501 cctgcacatc ggtccacggc gccgcgtgaa ggcgcgaacg caatgccgga tgcttttcga
     7561 gcatcgcatc gaggtcgccc gtcagcacgc gccgcgacac gttgcctgcg cggagcggca
     7621 gccgcgcggc gagcgcggcc gcgacgatcg gctcgtgcag cggccatcgc cagcccgtgc
     7681 ggtcgagctc gcctgccgtt gccacccgct cggcgtcgcc cccggcgttt tgcgccgcgc
     7741 ggagcgcctc gtcgcggttc agatcgaact gctccatcac gaccgcggga tgcgcgccgc
     7801 gttgggcaat ttcggaaagc agccgcgcgc gaatcgcatg atgcgcaggg tcgtcgtgga
     7861 tttcgccgag cagccggtaa cgcgcagcgg cgagatcggc gacgagcgcc gcctcggtca
     7921 cgaagcgctg cgcgtgcacg tcgtagattc gcgcgtcggg cggcggccgc ggcggcgccg
     7981 gcgaacaagc ggtcagcgtt gccgcgagca gcgcagcaaa gctaacgcaa cgatgcgttg
     8041 acgcctccga gcgccgcatt ccccggcacg aggtcggtgt caccgagcgc gttcagcggc
     8101 actccgacgg tcgacagcgc cgcatgcatg tcgtcgctct gctcgtcgac gtacagaagg
     8161 ccggtaacca cctccccgcg cgcccgcctc gactcgaggt aggccatcgc gccgaccttg
     8221 tcggaaggat cgtaactggc gtcgagcttg gcgagacgca cggtgctgcc gtcgtgcagc
     8281 tgcacctcgc gcacgccgcc ttccgggtag tcggtctcga tgggcgcgcg cgccggcatg
     8341 aaatcgagcc ggttcaccgc ctcgttgtgc tcgcgcacat agtcgtagct cttcgtcgat
     8401 ccgctgtggt tgttgaacgc gacgcacgga ctgacgatgt cgatgaacgc ggcgccgcga
     8461 tgcgcgacgg ccgccttgat cagcggaacg agctgcgcct tgtcgccgga gaagccgcgc
     8521 gcgacgaacg tggcgccgag cgccagcgca agcagcacga ggtcgatcgc ctcgtcgctg
     8581 ttgacggcgc cgcgcttgct cttgctgccc ttgtcggcag tggccgagaa ctgccccttc
     8641 gtcaacccgt acacgccgtt gttctcgacg atgtacacca tgtcgacacc gcggcgcagc
     8701 acgtgcgcga actggccgag gccgatcgat gcggaatcgc cgtcacccga cacgccgagg
     8761 tagatgagat cgcggttggc gagcgcagcg ccagtgagga ccgacggcat ccggccatga
     8821 acgctgttga agccatgcga attgccgagg aaatacgtcg gcgtcttcga cgagcagccg
     8881 atgccggaca gcttggcgac gcgatgcggc gcgatgtcga gctcgtagaa cgcctggatg
     8941 atcgcggccg agatcgagtc gtggccgcag ccggcgcaaa gcgtcgagat ggcgccttcg
     9001 tagtcgcgtc gcgtgtagcc gagcgaattc ttcggcaggc ccgggtgatg gatcttcggc
     9061 ttcgcgagat aggtcatgcc gcgcggcctc ctgttctgcg gatcgtcagc ggcttcacat
     9121 tgtaaagtcg cgccttctcg ccgatctcgc gggtgatgaa ccgctcggtg atcggcgtgc
     9181 cgtcgtagtg aagcaccgac gtgagcttgg ccgggtcgat gccgcattcg ttgacgagca
     9241 gcatcttgag ctgcgcgtcg cggttctgct cgacgacgaa cacgtgctcg tgcgccgcga
     9301 tgaactcggc aaccgtgtcg tcgaacggga atgcgcgaag ccgcatgcgg tcgaggtgca
     9361 cgtcctggtg cgcgagctcg gcgagcgctt cggccattgc ggcacccgtc gagccgaacc
     9421 agacgacacc ggccgtcgtg gcctgctccg ccctggtgat cacgggcttc ggcagcagcg
     9481 ccttcgcggt ctcgaacttg cgcaagagcc gctgcatgtt gtcgacgtac gccggcccct
     9541 cctccgtgta gcgcgcgtag cgatccttgg tggtgccgcg cgtgaaatag ctgcccttcg
     9601 ccgcgtgcgt tccaggatac gtgcggtagg caatgccgtc gccgtcgacg tcgaggtagc
     9661 ggccgaaatc gcggccggcg tcgagatcgg ccgccgtcat caccttgccg cgatccatcg
     9721 tgcggcggtc gtcccacgcg agcggctcgc agagccagtc ctgcatcccg atgtcgaggt
     9781 cgagcatcac gaacaccggc gtctgcagcc ggtcggccag atcgaacgcg agcgccgcga
     9841 actcgaagca ttcgcgcggg tcctcgggca tcagcagcac gtgcttcgtg tcgccgtgcg
     9901 acgcgtaggc gcaggacaga agatcggcct gctgcgtgcg cgtgggcatg ccggtcgacg
     9961 ggctgccgcg ctgcacgtcg aacagcaccg cgggaatctc ggcgaaatac gcgaggccga
    10021 gaaactcctg catcagcgaa acgccgggac ccgacgttgc cgtgaacgcc cgtgcgccgt
    10081 tccatgcggc gccgatcacc atgccgatcg acgcgagctc gtcctcggcc tgcacgatcg
    10141 cgtagcgatg gtcgcccgtc accggatcgg tgcgaaactt gcggcaccat ttcgcgaagc
    10201 tctccgcgag cgacgacgaa ggggtgatcg gataccacgc ggcaaccgtt gcgccgccgt
    10261 acacggcgcc gagggcggcc gccgagttgc cgtcgatgaa gatgcgctcg cccaccgcgt
    10321 cgcggcgcgc caccgacagg ccgatcggac agtcgaaatg ggcgagcgcg tgatcacggc
    10381 ccatcgcgag cgccttgcga ttcgattcga gcagcgcctc cttgccgcga tactgctcgg
    10441 ccaacaggcg ctcgatctcg gcggcgtcga tgcccagcag cgcggacagc gcgccgagat
    10501 agacgatgtt cttgaaaagc tgacgctggc gcacgtcgct gtacgcggcg ttgcagatcg
    10561 ccgtcagcgg cacgccgagg agcgccacgt cgtcgcggaa tttcgacggc ggcagcgatt
    10621 tcgtcgaatc gtagaacaga tagccgccgg cctcgatctc cgcgacgtcc ttgtcccagg
    10681 tctgcggatt catcgccacc atcaggtcga caccgccacg gcgtccgcgc cagcccgctt
    10741 cgacgacgcg cacttcgtac cacgtcggca ggccctggat gttcgacggg aagatgttgc
    10801 gcgccgcaac gggcacgccc atccgcagga tcgcacgcgc gaaaaggccg ttcgcggatg
    10861 ccgatcccga gccgttgacg ttcgcgaact tgacgacgaa gtcgttgacg cgggtgaggg
    10921 gcgacggtct catctcaatc cgccgccagt gccggctcgc gccgcttgcg gccggcatgc
    10981 gcccgatcgt cggcgtgcgc catgtcgagc aggaacttct gcatgtccca cgcaccggtc
    11041 ggacaacgct cggcgcaaag cccgcaatgc aggcacacgt cctcgtcctt cgccatgatg
    11101 cggcccgtct tcagcggcgt cgagacgtag agcgcctggt cgacgttgag tgccggagcc
    11161 atcaagcgcg atcgcaggtc ggcctcgtcg ccgttcgtgg tgaacgtgat gcagtccatc
    11221 gggcagatgt cgacgcaggc gtcgcattcg atgcaaagcc ggtcggcgaa caccgtctgc
    11281 acgtcgcagt tgaggcagcg ctgcgcctcg gcatacgcga gcttcgcgtc gaagccgagc
    11341 tcgacctcgg tgcggatgtc cttcagcgcg atcgtcacgt ccttgagcgg aacacgatag
    11401 cggcgatcgc tgtggatcgc gttgtcgtag ctccactcgt ggatgcccat cttttgcgac
    11461 atcaggttca cgtgcggcgg cgggcgatcg gaaagcggct cgccgcggca ttggaggtcg
    11521 atcgatatcg cggcgtcgtg accgtgcgcc accgcccaga tgatgttctt cgggccgaac
    11581 gcggcgtcgc caccgaagta cacgcccgaa cgcgtcgacg ccatcgtcgt gcgatcgacg
    11641 atcggcatgc tccactcgtc gaaggcgatg ccgatgtcgc gctcgatcca cggaaacgcg
    11701 ttttcctggc cgatcgcgac gagcacgtcg tcgcagggaa aatgcaccgg cggctcgccg
    11761 gtggcgacga gctgccggcg cccgcgcgca tcgcgttgcg ccgcgacctt ctcgaacgtg
    11821 acgccggtca ggcggccgcc gtcatgcgtg aattcgagcg gcacgaggta gttgtggatc
    11881 ggtattccct cgtgcatcgc gtcttccttt tcccacggcg acgccttcat ttcgtcgaag
    11941 cccgagcgga cgacgacgtg cacctcttcg ccgccgagcc tgcgcgagct gcggcagcaa
    12001 tccatcgccg tgttgccgcc gccgagcacg acgacgcgcc tgccgatcga cgtgatatgg
    12061 ccgaacgaaa ccgacgacag ccagtcgatg ccgatatgaa tgccggcggc cgcctcgcga
    12121 cgcccgggaa tgtcgaggtc acggccccgt ggcgcgccgc tgccgacgaa cacggcatcc
    12181 cagcgctcgg tcgcttcggg ggcgagcagg cgggcaagcg attcgatacg cacgccggcg
    12241 cggaaatcga tgccctcgtc gagcacgtag ctcacttcct cgtcgatgac ccggtcgggc
    12301 agccggaatt tcgggatctg cgtacgcatc atgccgccgg ccttcgcgtc ggcgtcgaac
    12361 acgacgcatt cgtagccgag aggggcgaga tcgcgcgcca ccgtcagcga cgcggggccg
    12421 gcgccgatca gcgcgatgcg cttgccgttg cgttgcgcgg gggggcccgg cagccgcgga
    12481 cggatgtcct ccttgttgtc ggccgcgacc cgcttcagcc ggcagatcgc gaccggctcg
    12541 ggcttggcgt cggtgcggcg atgctcggcc gatccggcct cgacgcgaac gcgccggcag
    12601 gcgggctcgc agggccggtc acaggtgcga ccgaggatcc cggggaagac gttggacttc
    12661 cagttgacga gataggcatc gccgtagcgg cccgcggcaa tgagccggat gtattcggga
    12721 acgggcgtat gggccgggca ggcccattgg cagtcgacga ccttgtggaa atagtcggga
    12781 tgcttgatgt ccgtcggttt caatccggct cctcgaacgc acgtcgcagc ggtgcgcacc
    12841 acgatgatgc aagcgaaacg cgcctcggat gctacgcctg cccgcacagc aagaaaagcg
    12901 ccgggaacgc aagcgcttga aacgcgtcga tattttgcaa cgcccgccgc atgccgcacc
    12961 aggtcgagca cgcgcgctgc tgcgctagaa tgaccttttg cgccgcggtc ctgcagatcc
    13021 gatgcaaaaa ggcgcccccc tccccgcatg aacgcacccg aacgcccctc ggttcgcctc
    13081 acccgtttct cgcacggcgg cggttgcggc tgcaagatcg cccccggcgt gctgcagcag
    13141 atcctcggac gtgtcgatcg cacgctgatt cccgatgcgc tgctcgtcgg catcgagacg
    13201 gccgacgacg ccgcggtcta tcggctgaac gaacggcagg ccatcgtcgc gacgaccgac
    13261 ttcttcatgc cgatcgtcga cgatccgttc gatttcggcg cgatcgccgc caccaacgcc
    13321 atttcggacg tctatgcgat gggcgcaacg ccgcttttcg cgctcgcgat cgtcggcatg
    13381 ccgatcgacg cgctgccgct cgatgtgatc gggcgcatcc tcgagggcgg acaatccgtt
    13441 tgcgcgcgcg ccggcattcc ggtggcgggc ggccacacga tcgatgcggt ggaaccgatc
    13501 tacggcctcg tcgcgatcgg cgtcgtcgat ccgcgcgcgc tgaagcgcaa cgccggcgcg
    13561 cgcgccggcg atgcaatcgt gctcggcaag ccactcggcg tcggcatcta cagcgcggcg
    13621 ctgaagtgcg attcgctttc cgccgacggc tacgcggcgc tggtcgcgac cgcgaccgcg
    13681 ctcaatacgc cgggcatcgc gctcgcgagg ctcgacgacg ttcacgcgct gaccgacgtc
    13741 accggcttcg gcctgctcgg ccacctgctc gagatgtgtc gcggtgcgaa ccttgccgcc
    13801 accatcgagt tcgagcgcgt tccgttgctc ccgcaggcga tgagtctcgc ctcggcgggc
    13861 ttcgtgaccg gcgcatcgcg ccgcaatttc gacgccttcg gcaacgacat cgtctcgggg
    13921 gagcgcatcg gcgggcgcga gcgtgcgctc ctcaccgacc cgcagacgtc gggaggtctc
    13981 ctcgttgcct gtgcgccgca cgcgctcgac gacgtgctcg acattttccg tcgcgacggc
    14041 ttcgcgtccg cggcgccgat cggacgtttc ggcgccggcg tgccgaggat cacgatcacc
    14101 tgacgcgatg ccaccgatgc cgatgccggg cgcaaccgcc ctcgccgtgc cgtcccttcc
    14161 gctgctgcgt cgctatcgca acgtgcgcgc acgcagcgcg acgctcgtcg caccactgtc
    14221 gcccgaggac tgtgcgctgc agtcgatgcc cgatgcgagc ccggcgaagt ggcacctcgc
    14281 gcatacgacg tggtttttcg agacattcgt cgtcgcgccg cgcatcgccg gctatcgcgc
    14341 gttcgatccg gcgttccgcg tgctgttcaa ttcgtactac cacgcggtgg gcgaccggca
    14401 tcctcgcccg gaacgcggca tgctttcgcg tccgccgctc gacgcggtgc tcgcctatcg
    14461 cgcatccgtc gacgaggcga tgtacgaatg ctgtgaacgc gcacgaaacg atgcggcact
    14521 tgcctcgctg atcgagctcg ggctcgcgca cgaggagcag catcaggagc tgatcctgac
    14581 cgacgtgctg catctcctgt cacgcaaccc gctcgctccc gcctacgatc cggagggtcc
    14641 ggcgccggtg catcgcgcac gcgacatggg ctggatccgc tatcgggccg gactcgcgaa
    14701 catcggccac gccggtgacg gcttcgcgtt cgacaacgaa atgccgcgcc atcgcgtctg
    14761 gctcgaatcg ttcgcgctcg catcccggcc ggtgacgaac gcggagtacg cggcattcat
    14821 cgacgacgcc ggataccgcc ggcccgagct atggctggca cttggctggg atacggtcac
    14881 ggcgggaaac tggcaggcgc cgctctactg gattcgcgac gacgatcgct ggcaatcgtt
    14941 cacgctgcaa ggtgtcgccg atctcgatcc gcatgcgccg gtggcgcaca tcagcttctt
    15001 cgaagccgac gcctacgcgc gctgggcggg tgcgcggctg ccgacggaat acgagtggga
    15061 agccgtcgcg gcgcgcggca ccgatgcgcg aaccggcgat ccatgggccg gcagcctcgg
    15121 cacgaccgtg acgcttgcgt gcggcgatgt gtgggagtgg acgtcgtcga gctacgcgcc
    15181 ctatcccggt tttcgggcgg cgcctggcgc cgtcggcgag tacaacggca agttcatgtg
    15241 caaccagtac gtgctgcgcg gcagttcatc ggcgacacca cccggccacg cgcggatcac
    15301 ctatcgcaat ttctttgcgc ccgatgcgcg ctggcagtgg agcggcgtgc gcctcgcccg
    15361 cgatcacgca cccgcgccgt aatacacggc gaaatcaccg gcgtcatcct gccagcagcg
    15421 cacgtcgcgc aatccggcac gctcgagcat cgccacgaat tcgggcggcg cgtacttgta
    15481 cgagttctcg gtgtggatgc gctcgccggc cgcgaacgtg cgcgcgatgc cgtcgacgac
    15541 gaccgtctgg cgcgtgcgcg cctcgagatg catttcgacg cgcgaatccc gctcgttgta
    15601 gaacgcgaca tgcgcgaatg cggcggggtc gaatcgcgtg ccgaggatgc ggttcacgtg
    15661 cgcgagagca ttgcgattga acgcggccgt gacgccggcg gcgtcgtcgt atgcggcggt
    15721 gagtcgcgcg gcgtcctttt tcgtgtcggc gccgatcagg agcccgctcg ccgctccgcg
    15781 ggcgtgccgc cggattgcct gcaggaagtg ctgcgcctcc accggcgtga aattgccgat
    15841 cgacgagccg ggatagaaaa acgtcaccgc acccggcccg agatcgcgga agagatcgag
    15901 gccgtgcgca aagtcggtga gcaagccgcg cacttcgatt cgcggatgtg cgttcgcaat
    15961 tcgcgcgagg gccggtgcaa gcgcctcggg cgcgatgtcg acgccgacgt agcgccaggg
    16021 ctgcaggaac gggagccagc gcgccgcctt ctcgcagtca ccggccccga ggtcgacgaa
    16081 ctcgcggccg gtgcccgcgg cggcggcaat gtcgtcgcga tgacgctcga agatcgccgc
    16141 ttcggtgcgc gtcgggtagt actcgggaag ccggcagatc tcggcgaaca gcgccgaccc
    16201 acgcgcgtcg tagaagtatt tcggcgcgat cgacgcgggc gtggacagaa gcccgccgac
    16261 gagcgcgcgc cgctcggcgc ccgcgtcgtg cacgcgacga tcgcaaagcc gctgctcgag
    16321 tgccggcatg agcgcggcgt cgggcgttgc atggagcctg tgagcgccag cttgcatcgg
    16381 ttctctcggt aacctttacc gggtttcgcg ggtacgcctt gcatcggcgc acgtcatctc
    16441 gcaaagcacg acgcttgcat tgcgtcgtaa ccattcgacc ggcacgtcgc ttgcaagatc
    16501 ggcgccatgc tccgcacggc gatgacagtc ctggcggcgc tggcgttcac ggccggcgcg
    16561 catgcgcaga tcgatccggt gcgccgcgac ctcgtcgaat tcggctacgg ccagccgctc
    16621 gcgggccacg cgccggtggg cgcgtacgtc ttctattatc tgagccggcc gcaattcggc
    16681 gacgcggatc atgcgctgcg gctcgcggtg tcgcccgtct atctcgacgg cgaatgggcg
    16741 tggcgaaatg ccctcggggc gcacaccgat ctcgcgatcg gcgtcagcgg cggcggcttc
    16801 gcctcggatt acaacgagat tcgcgcggga cactggcttc gcgaccagtc gttcaccggt
    16861 cacgacggcg gtgtcgacgt cagcgcgtac cacctgttcg atcccggtgc gcgcgttccg
    16921 gtgtacggca tcctgcgcgg tgacttccgc tacgtcgcct tcgagcgcga cagcgaaaat
    16981 ccgccgaact tcatcctgcc cgacaaccag ccggtcgcaa gcgttcgcgc cggcttgcgc
    17041 gccggcggca tcgagccggt gctcgcaccc gacgtcgcgg gcgaactgtc ggcgtggtac
    17101 gaaacccagg tgcggctcaa tgccgggacg tacggcttcg acggcgatcg acgcgtgcag
    17161 cgcaccgtgc agcgcttgtg gggtcgcggc ctgctcgttt acacactgcc gcaatcgcgg
    17221 caccgcgtcg cgttctcgct cgtcggcggc acgtcggtgc atcccgatcg gctgagcgcg
    17281 tggcacatcg gcggcgtgct gccgctcgac agcgaatttc cgctcgtgct gccgggatac
    17341 tacgagggcg agttctccgc gcgcaacttc gtgctcgcgg gcgccgatta cgcggtgccg
    17401 ctcgaccgcg gcgagcactg gcaactgggc gtcggtgcgg cggtggcacg gatcggcttc
    17461 acgccgggac tcgaaggccc gcgcgcgtgg aacagcggca tgagcgcctc gctgggctat
    17521 gcgccggaca cgaaggcgtg gaaggcgtcg ctcgtctacg gtcgcgccct cgacgccgtt
    17581 cgcaacggac accggggtgc cgacagcgta acgctgctga tggaattcga cctcgagcgc
    17641 atgggctatc tgccggcgga tgcgagccgg ccgcggcgtc cggattgaag tcagcgccgt
    17701 gtcgcgatcc cgcgcacggc gacgcggtac gcgatcagtg cgccggcagc cgcgaaggca
    17761 agcgcataca gcgcgagccg gcgcgtgtcg tcgagggcga acagaagcgt gacggccacc
    17821 ggcgcgaagg ctttcaggat gaattgcggt tgcgccagcc ggccaagaag cgcgccgtaa
    17881 tcgcgcgcgc caaaaagaac ccccgggacc gtgccgcgca cgatcgtcat cacgccgttg
    17941 ctccatccgt acagcagcgc gaacgcgagt gccacgatcc acacgccacg cacgaggcac
    18001 agcacgatca gcgccgacgc gagcagcgtg aacgcgagcg tgcctacggt gagcggcgac
    18061 cagcgattcg agaacgcgaa ttccatcacg cgtccggcca cttgcatcgg tccgatcagc
    18121 gcgcccacga gcaccgcatc gcgcgccgcg agtccgccgg aggtgagcag cacgacgagg
    18181 tgcgccgaca ccgcggacgc gaggaacgcg gcgaccgaga gcgccgcggc gagccaggcg
    18241 aaggtgccgc ggcgtgtcag cgccgtctcc gccgttcccg cgctcgccgg tgcgaccgac
    18301 cgatgtgcgg acgatggaac gaacgcgagg tgcagcggca cgcacacgag cgcgttcaat
    18361 gctgcatgca cgccgaacgc aacccgccat ccgtgcgttt cgaggaggta ctgcgacagc
    18421 ggccagaaca cggtgctcgc aaagccgccg aacaacgtca gcgcggtcac ggcgcggcgg
    18481 tacgaggcac ctgacacgcg aaagagcgtc gcgaacgccg gatcgtaaag acaggccgcc
    18541 atcgcgaccc cggccacgag ccagccggcg agcatcatcg cgccgttgac cgacgtcgcg
    18601 agcagcgcgc aggcaagcgc gccaagtgcc gatccgccgg cgagcaccgc gcgcccgccc
    18661 cgccggtcga tgcgcgaacc aacccacggc gacgcaatgc ccgacaggaa cagcccggcc
    18721 gtgaagctgc cgtacagcat cacgtcgctt acccgcaact cgcgcgccat cggcggaccg
    18781 agcaccgcga tcgtgtagaa gagcgtgccc cacgagacga tctggccgat gccgagcgcc
    18841 gtgacgagcg ggcgcaacgc aaccggtgcc acccggtcac tgcgcatgcg tcgatcggcg
    18901 ttcgccgggc gtgagcaggt ccgcaagtgc gacggcgacg accccgcttg cacgcgcaag
    18961 gtcgtcgagt cgcaggcgct cgtcggcgcg atggccgttc gcctcggcca gcgtgcgcgg
    19021 cccggcgccg tagagcaccg tcgggacgcc ggcggcagtg tagtggcgcg cgtcggtgta
    19081 gatcggcacg ccctgcagcg gaagcacctc gccgagacat cgccgcgcgg cgcgctgcag
    19141 tgcgccgacc aggcgctcct gcccggcgat cggcacgaac ggccgcgcga gcaggatacg
    19201 gcgtacgcga acgcccgcgc cgggccactg ctgcgcgaaa tcgtcgatcg catcgcgcag
    19261 cgtcgcttcg acgagccccg ggttctcctc cggaatgatg cgccggtcga tgcgaaagcg
    19321 aacgcggtcg ggcacgacgt tcgtgttgat gccgccgtcg atcaacccga cgacgagcgt
    19381 cggatgcgtg atgccgggaa tcgtggagcg aatcccggtg tacgtgccgc gcagcgcgta
    19441 gagatcggtg aggatgccgg tggccgcctc gagcgcatcg accccgcgct cgggctccgc
    19501 cgcgtgaccg gacatcccgg tgacctcgac ttcgagatgc aggcagccgt tgtgcgcggt
    19561 ggtgatggca tacgcaaagc cggcgctgat cgtgtagtcg ggccgcgtga gtccgtgcgc
    19621 gaggagccat gccggtccga tctcgccccc cgcctcctcg tcgtaggtga aatgcagctc
    19681 gaccgtgcca tcgaatgcgc ggccgtgctc tgccgcatgc tgcaatgcgc gcagcgcaaa
    19741 cgcatacgtc gcgaaatcgg acttcgacac ggcgacgccg cgaccgtaca tcacgccgtc
    19801 gcgcaccgcg gcgccgtagg ggtcggtggt ccagtgcgat ccgggcggga cgacatcgcc
    19861 gtgggcgttc attgcgatca ccgggccggc gccgcgtccg aacgtcgagc gcacgatcag
    19921 gttcgtgcac gacaccatgc cgtgcgcccg cacctcgtcg tcgggcacgc ggtgccgctc
    19981 gacgtcgagg cccatcgtct cgagcatcgc cgccgccttc gccgcgtgcg gcgcgcaatc
    20041 gccgggcggg ttgtcggacg gctcccggac gagcgctgcg agaaacgctt ccgcttcgcc
    20101 gcggtgctcc gcgagccagc cgatgatgcg atcgtcgtcg atgcgttgcg cgccggccac
    20161 gcttcagcga tgccggaatg ccggctttcg cttctcgacg aacgcgcgca tgccttcctt
    20221 ctggtcgtcg agcgcgaacg tcgaatgaaa ctcgcgccgc tcgaaggcga gaccctcggt
    20281 cagcgacgat tcgtacgcgc gattgatcgc ttccttgatc ttcatgacca ccggcagcga
    20341 gaacgaggcg atcttcgcgg ccagtgccat cgcttcctgc tcaagcgcgg cgagcggcac
    20401 cacgcgtgcg acgagtcccg cgcgctccgc ctcctgcgcg tcgaacatgc ggccggtcag
    20461 gcaccactcc atcgccttcg acttgcccgc ggcacgcggc aggcgctgcg tcccgccgaa
    20521 gcccggcatc gttccgatcg tgatttccgg ctggccgaag cgcgcattgt cggcggcgat
    20581 gatgatgtcg caggccatcg cgagctcgca gccgccgccg agcgcgtaac cggcgaccgc
    20641 ggcgatcacc ggcttgcgca cgcggcgaat gccttcccat tcggcggtga tgtaatcgtc
    20701 gccgtaaacc ttcgcgtaat cccattcggc catcgcaccg atatcggcac ccgcggcaaa
    20761 cgccttgtcg ttgccggtga tcaccgtgca accgatcgcc ggatcggcgt cgtacgcaaa
    20821 cagcgcgtcc gctatttcgc gggccacctg gttgttgagc gcattcatcc gctgcgggcg
    20881 gttgatgcgg atgaggccga cgcgctcgcg cgtctcgatc agcagatgtt cgggcatcgt
    20941 ggcagccttc gaagcggtgt cggaggtgca gttgcgggaa agacccgcga cgccggaaac
    21001 acacctccga caccggcgtc gccgtgacgc ataataacga atcggacaac gagccgcgcg
    21061 acacaacgag gacgacgcca tgaaaaaggt ctacgcgaat gcgacggaag cgctctcggg
    21121 gcttcttgcg aacgacatca cgatcgccgc cggcggcttc ggcctgtgcg gcattcccga
    21181 gaatctgatc caggcggtcg tcgacagcgg cgtcaagggg ctcacgatcg ccggcaacaa
    21241 cgccggcgtc gacgacttcg ggatgggatt gctgctcaag ggtcaccagg tgaagaaggt
    21301 gatcgcgtcg tacgtcggcg agaacaagga attcgagcgg caggtgcttg cgggcgagct
    21361 tcaattgcag ctcacgccgc aggggacgct tgccgaaaag ttgcgcgcgg gcggcgccgg
    21421 cattccgggc ttttatacgc ccaccgccgc cggaacgctg ctcgccgaag gcaaggaaac
    21481 gaaggcgttc gacggccgcg agtacgtgct cgaggaaagc attcgcgccg acgtgtcgat
    21541 cgtcaaggca tggaagggcg atcgcgccgg caacctcgtt tatcacgaaa cggcgcgcaa
    21601 cttcaatcca atgattgcga cgtgcggcaa ggtcaccgtc gccgaagtcg aggagctcgt
    21661 cgatgtcggc gagctcgatc cgaacggcat ccagacgccc ggcatctatg tcgaccgcat
    21721 ctttcgcggc acgaattacc agaagcgcat cgagttccgc acggtcgccg gcaccgcggg
    21781 caagaaggat tcgccggtgc gcgaaatgat ggcgcggcgc gcggcgcgcg agcttcgcga
    21841 tggctattac gtcaatctcg gcatcgggat cccgacgctg gttgcgaact tcatcccgca
    21901 aggcatcagc gtgacgctgc aatcggagaa cggcctgctc ggcatcgggc cgttccccgc
    21961 cgacaacgag gtcgatcccg acctcatcaa cgccggcaag cagacgatca cgacgcttcc
    22021 cggaagcagc ttcttctcct ccgccgactc gttcgcgatg atccgcggcg gccatatcga
    22081 cctgtcgatc ctcggcgcgc tggaggtcgc ccagaacggc gatctcgcga actggatggt
    22141 gccgggcaag atggtgaagg gacccggcgg cgcgatggac ctcgtctcgg gcgtgaagcg
    22201 cgtgatcgtg ctgatggagc atacggcgcg cgacggcagc ccgaagattc tcgagcgctg
    22261 cacgctgccg atcaccggca agaacgtcgt cgatctcatc gtcaccgatc tttgcgtgtt
    22321 cgaggtgaag cccgatggcg aagggctcgt gctgcgcgag ttgcacgagg gtgtgacgct
    22381 cgatgacgtg aaggcgaaga cgggctgcgc attcgaggtg gcgctggaac ggcgatgagc
    22441 aagcgcggga ggatcgtgca agcccggcgt cgggagcgaa cgtgagggtc gcgatcctcg
    22501 acgactatca cgacacgctg cggacactcg atgcgttcgc gaagctcgcc ggtcacgacg
    22561 tcacgatctt caacgatcat gtgcaggacg tcggccggct cgccgcgcgg cttgccgacg
    22621 ccgaggcgct ggtgctgatc cgcgagcgca cgcagatccg cgcgccgctg ctcgaacgcc
    22681 tgccgaagct acgcctcatc agccagcgca gcgtctatcc gcacatcgat atcgacgcct
    22741 gcacgcggct cggcatcgtc gtgtcgtcga gccagcacgc gggcacgcca tcgtacgcgg
    22801 ccgccgagct cacgtgggga ctcgtgctcg cggcgatgcg cgacattccg cagcagatga
    22861 atgcgctgaa ggccggcaag tggcagatcg gtgttggcca cacggtgcgc ggcaagacgc
    22921 tcggcgtcta cggctacggc aggatcggcg ccgtcgtggc gggatacggg aaggcgttcg
    22981 gctgcaacgt cgtcgtctgg gcacgcgaag cgtcgcgcga gcgggcgcgg gccgacggct
    23041 acgcagccgc cgacagcaag gcggcgtttt tcgccgaatc ggacatcctg acgctgcaca
    23101 tgcggctcgt cgacgcgacg cgtggcatcg tcaccgcagc cgaccttgcg cggatgaagc
    23161 ccaccgcgct gctcgtgaac acgagccggg ccggcctcgt cgcacccggc gcgctcgtcg
    23221 aggcgctgcg caacggccgt cccggcatgg ccggcgtcga cgtgtacgac caggaaccgc
    23281 tggtcgaccg gaacgacccg ctgctctccc tcgacaacgt cgtctgcacg ccgcacatcg
    23341 gctacgtcac gcgcgacgaa tacgaggtcc agttcgccga gatcttcgat cagatcgtct
    23401 cgtacgccgc cggcaagccg atcaacgtcg tcaatcccgg cgtgctcgat catatgcgcc
    23461 gcgcatcctg atcttcccgg catgccgcac ctcgagctcg acgggctgac gaagcgctac
    23521 ggcgacgtca cctcggtcga cgccatcgac ctcaccgtcg agcgcggcga attcatctgc
    23581 ctgctcgggc cctccggttg cggcaagacg accacgctgc gcatgctcgc cgggttcctc
    23641 atccccgacg ccggggagat ccgcgtcgcg ggcaagacgc tctcgtcgtc gtcggccgtc
    23701 gtgccgcccg agcggcgcaa catggggatg atcttccaga gctacgccgt atggccgcac
    23761 atgacggtgc gcgagaacgt cggctacggc ctgcggatgc aatcggtgcc ggcgacggag
    23821 cgaaacggtc gcgtcgatgc gcttctcgac gcgacgcggc tgcgcgagca cgccgatcgc
    23881 tatccggcgg aattgtcggg cggccagcag cagcgcgtcg cgctggcgcg tgcgctcgcg
    23941 cccaatcccg gcacgctgct cctcgacgag ccgctgtcga atctcgatgc gaacctgcgc
    24001 ggcgagatgc gcttcgagat acggcggctg cacgacgagt accgcaacac gtcgatctac
    24061 gtgacgcacg accaggtcga ggcgatgacg atggccgacc gcatcgtcgt gatgaacgcc
    24121 gggcgcatcg agcagatcgg cacgcccgag gacgtgttcg accgcccgca ttcgcgcttc
    24181 gtcgcccgct tcatcggcgg cagcaatctg ctcgaaggca cgcgcgtcga cggccggcgg
    24241 gtcgacgtcg cggggcatgc actggacgtc gggcacgggg agttcgccgg gcgcggcgag
    24301 ccgatgagcg tctgcgtgaa gacgcacgac atgcacatcg acgccgatgg gcgcgccgat
    24361 ggcacgaacg tgctgccggg cgtcgtgcgc agccaggcgt atctcggcag ccaccgcgat
    24421 tacatcgtcg acgtcggcca gccgctcttg atcgcggcgc ccgcagcgct caacataccc
    24481 gccggatcga gcgtgacggt gcgctttcgc cccgaccgtt gtcgcggcat catccgataa
    24541 caaagccatg cgccatgggc gcgcggcaaa cgaagtcagg aggaggagat catgcgaatc
    24601 acccggattg cggcgtttgc cgccgcactg ttcacctgcg ccgtcgcgat gcaggcgctg
    24661 gcgcaggaaa ccacgcccgc gatgctcgcc gcggccgcga aggagggcaa ggtcgtctgg
    24721 tacacggcgg tcgacgtgaa ggtcgccgag tcggtggcga aggtcttccg tgccgagtat
    24781 ccgcagattg cgctcgaagt cgagcgctcg ggatccgagc gcgtcttcca gcggatcaac
    24841 caggaattcc agtcgaacat tcacaacgtc gatgtcgtga attcctccga cgcgtcgcac
    24901 ttcctgctct ggaaacagca gaagctgctc gccaagcacg tgccgcccga cgtgcagcgc
    24961 tttgccgcgc agtacaagga ccccgacggc tactacgcgg tgtggcgggc gtcgctgtcg
    25021 gtgatgggct ataacacgaa cctcgtcaag cccggcgacg cgccgaaggg ttatcgcgac
    25081 ctgctcgacc cgaaatggaa agggaagctc gtcaagtcgc atccgggcta cagcggcacg
    25141 agcctgaccg gcacgttcgc gctcgaaaaa gtgctcggct gggattactt cgaaaagctc
    25201 gccaggcagg gcgtgcagca gttgcagtcg acgacggcgc cgccgaagag catcgccagc
    25261 ggcgagcgcg cggtgatggt cgacggcaac gagtacaaca tgttcatcga gatccaggcg
    25321 aagagcccgg tgaagatcat ttacgcggtg gaaggcacgc cgttcgtcac gtcgccgacg
    25381 gcgatcttcg ccgaggcgcc gcatccgaac gcggcgcgcg tgctgcagaa cttcctctat
    25441 acggcgaagg tccagcagct cctcgtcaac gaggggggat tgcgctcggt gcatcccgat
    25501 gtgaaggaac cggccgatcg cacgccgctc aaggacatca agctgctggc cgacgatccg
    25561 gtcgcgatgc tcccgcaggt ggccgagatc aagaagcgct atacggcgat ctttggcaac
    25621 tagcggcgcc gtccgcgggg cgtcgttcga cgccagcacg gcggtctccg gcgccatcgt
    25681 gctgctgctg gcggcgctcg tgctgatgcc gctcttctgg ctcacggtca cgagcttttc
    25741 gaatgacgca aaggcattca cgctcgc
//
LOCUS       DASSVX010000230        17127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_742929, whole genome shotgun sequence.
ACCESSION   DASSVX010000230 DASSVX010000000
VERSION     DASSVX010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 17127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17127
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742929"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1694)
                     /locus_tag="VFO53_10055"
     CDS             complement(<1..1694)
                     /locus_tag="VFO53_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET8876658.1"
                     /translation="MTKAFLRVPLRAALAIVAVALALPVQADVPVTAVPPADLRPGAT
                     QKSAEAQLVLPATARGLRVSLPEPTPAERATLKARNASAASGTAKAGNLKGPLALAFP
                     RNPPAGSSAISLAGLAWVSLPDGSRAAKIDVVSPGAAALRVALQLPAMDTGVSLRFAG
                     NGARAQVFGPIPANAIAGDTQRFGVFWSPVLDGDTATIEIHADAGTSTSDALLTIARI
                     SHQVVAPASIGKLDAKAVQDIGTSGACEIDVACVSPQSSAFVDSTKAVAALEFTREDG
                     FTYLCTGQLLNDSLASNTPYLFSANHCLDSAMAARTLNTYWFFEALACGNDTVPAYVQ
                     QTAGAAMLARSPDSDWALVRLNAAPPAGAKFSGWRAEPIPSAATVSVIHHPEGDLKKW
                     SQGAAQGYQLYTDGSSFAQVQYSRGTTEPGSSGAGLLTFNNAGYYEVRGGLWRGAASC
                     QNPTGIDEYSRLDGMLALTRQYLTPDSKGTPGSAVAVEYYNRTLDHYFITISPVELDA
                     LDSGAISGWERTGLRFLAYAAPAAGTNPVCRFYRTPGFGDSHFYSASPSECQAVID"
     gene            complement(1793..2686)
                     /gene="rfbD"
                     /locus_tag="VFO53_10060"
     CDS             complement(1793..2686)
                     /gene="rfbD"
                     /locus_tag="VFO53_10060"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HET8876659.1"
                     /translation="MARTRATILLTGARGQLGRELAAALPAVGDVVACDRSTLDLADP
                     AAIVEAVRRVRPQIIVNAAAYTGVDRAETERETALAINARAPGILADEARRAHATLIH
                     YSTDYVFDGRQTAPYDESAPTQPINAYGESKLAGEQAIGASGAFAITLRTSWVYGRHG
                     QNFLVTMQNLAARRSHLRVVADQTGVPNWSRSIARATSRMIGEAGEHLADRAGLYHLS
                     AAGSTTWYGFARAILEGCGVEITPIASVDYPAPARRPAYGVLDATRFARTFGFALPDW
                     QTLLQECLRADAEPPSGAAVH"
     gene            complement(2702..3244)
                     /gene="rfbC"
                     /locus_tag="VFO53_10065"
     CDS             complement(2702..3244)
                     /gene="rfbC"
                     /locus_tag="VFO53_10065"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET8876660.1"
                     /translation="MRVSPTSLPDVLLIEPRVFDDARGFFFESYNKRAFTEAGIDADF
                     VQDNHSRSRHGVLRGLHYQIEHAQAKLVRVTAGEVFDVAVDLRRSSPHFGRHVAVTLS
                     SQNRRMLFIPPGFAHGFVVLSDAAEFLYKTSDYWYPAHERTLLWNDAALGIAWPAQVT
                     PTLAAKDAAGVPFAAAEAYA"
     gene            complement(3244..4128)
                     /gene="rfbA"
                     /locus_tag="VFO53_10070"
     CDS             complement(3244..4128)
                     /gene="rfbA"
                     /locus_tag="VFO53_10070"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET8876661.1"
                     /translation="MHRKGIILAGGSGTRLYPVTQVVSKQLLPVYDKPMIYYPLSTLM
                     LAGIRDILLISTPDDTPRFAQLLGDGTRLGLSISYAVQPSPDGLAQAFVIGREFVGRD
                     ASALVLGDNIFYGHDLQPQLERASACAAGATIFVYPVADPERYGVAELDVTGRVKSLE
                     EKPSEPKSRYAVTGLYFYDNRVLDVAAGLAPSARGEFEITDVNRQYLAWDELACEVMG
                     RGMAWLDTGTHGSLLEASQYIETIERRQGLKIACPEEIAYRMRYIDAAQLEKLGASMA
                     KNGYGRYLLRLLEEPPLS"
     gene            complement(4121..5167)
                     /gene="rfbB"
                     /locus_tag="VFO53_10075"
     CDS             complement(4121..5167)
                     /gene="rfbB"
                     /locus_tag="VFO53_10075"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET8876662.1"
                     /translation="MILVTGGAGFIGANFVLDWFAQAAEPLVNLDKLTYAGNLRTLAP
                     LAANGEHVFVRGDIGDTELVRSLLERHRPRAIVNFAAETHVDRSIHGPAAFVQTNVVG
                     TFALLECVRAWWSDLPAQPRNAFRFLHVSTDEVYGSLGPGDPAFSETTPYAPNSPYAA
                     SKASSDHLVRAYHHTYGLPTLTTNCSNNYGPRQFPEKLIPLMIVNALSGKALPVYGDG
                     TNVRDWLYVADHCSAIRTVLDKGRPGETYNVGGSAEMANIDVVRSVCRLVQARRPGRD
                     CERLVTFVTDRPGHDRRYAIDATKIRDELGWAPAETFESGMQRTVDWYLGNGEWLASV
                     QNAEYRRWVDLQYA"
     gene            complement(5321..6343)
                     /locus_tag="VFO53_10080"
     CDS             complement(5321..6343)
                     /locus_tag="VFO53_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET8876663.1"
                     /translation="MTRDAPAGQRLLVTGAAGFIGMHVARALLDNGAAVTGVDSLDAY
                     YDVALKHARVNELRAFAGFAFEEIDLAEAAAAAALFARRFDAVVHLAAQPGVRYSLVN
                     PEAYVRNNLVAFAHVLEGCRLASVRHLVYASSSSVYGANHVLPFSEDQPVDHPVSLYA
                     ATKKANELMAHSYSHLYRLPTTGLRFFTVYGPWGRPDQAPMLFAKAILEGVPIDVFNA
                     GRMQRDFTYIDDIVEGVVRVLATVPSADGDGAPYAVYNIGNHEAVELDRFVATLERLL
                     GRRAIRNLRPMQPGDVRVTYASIERLARATGFAPHTPLDEGLARFVDWYRGYYRVGAP
                     SVPSRE"
     gene            complement(6394..7161)
                     /locus_tag="VFO53_10085"
     CDS             complement(6394..7161)
                     /locus_tag="VFO53_10085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET8876664.1"
                     /translation="MAEPLPVSLVVITRDAAADIADCLLSAPFASDALVVDSGSGDHT
                     VETARRTGARVITRAFLGFGRQKQFAVEEAVHDWVLCLDADERLSAALRESIVTLFAS
                     GEPPHAAYALARRNRFLGRWLAHGEGYPDWTTRLFDRRRARWSDDPVHEHVIAEGPVG
                     RLDGDLLHASAESLERYIAKQNRYTTLQADALHASGRRSSAFAMAAAPLARFLRFYVL
                     KLGFLDGAAGFAHIAIGAFASFLKHAKLRALDRAGRG"
     gene            7272..8255
                     /gene="waaC"
                     /locus_tag="VFO53_10090"
     CDS             7272..8255
                     /gene="waaC"
                     /locus_tag="VFO53_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HET8876665.1"
                     /translation="MSGEGSSVLVVRPSSLGDIVYALAAAADIRRARPGLAIDWVSEP
                     GFAPLVALCPDVRRVIPFGLRRWRRTPLARTTWRDICAFRADLRRTRYLAILDLQEQV
                     KGALIARAARGVRHGFDRASIREPLATLGDDIHHRVPRELHFLTRCRRLAAAALGYAN
                     VEPPRWNLRAPTIAPAMPDRPYAVVLHGTSRDDKLWPETHWRAVLDMLERAGIATVLP
                     WGSDAEEARSRRLAHGFAHAVVPPWLSLPEAAAMLAKAALAIGVDTGFTHLAAALGTP
                     SIAIFGATDPGRHGVACAGAHAQDLGDAGAPPAPDAVLAAAGEMLRRAPCC"
     gene            8261..9547
                     /gene="waaA"
                     /locus_tag="VFO53_10095"
     CDS             8261..9547
                     /gene="waaA"
                     /locus_tag="VFO53_10095"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HET8876666.1"
                     /translation="MAARLAYTLLWWLALPFLPLRLWWRGRREASYRLHIGERFGRYA
                     GAARAVGNDVVWIHAVSVGETRAVVPLVERLERDNPRRTILLTHMTATGRDTGRALFG
                     NRVVQTWLPYDLPFAVDRFLAHFHPRAGLLVETELWPNLAAAAARRRVPLFLINARLS
                     ARSARGYARIGPLVRPLLRSLSGVAAQTDDDAARLRALGARDVAVTGNLKFDADIAPA
                     MRERGRIFRDRFGIDRPVVVLASTRAGEEALLLDALARAALPPHALVVIVPRHPQRFD
                     DVAAMLASRAQHFVRRSDECAVPRDVAIVLGDSMGEMLAYYAAADVAFVGGSLLPLGG
                     QNLIEPIAAGVPALVGPHTFNFAQAARAAIAAGAARRVEDADQMWKVAAQLLADTASR
                     ERMCANARSFIAAHRGAADRLWTWLAPRIEQRPRRS"
     gene            complement(9553..10890)
                     /locus_tag="VFO53_10100"
     CDS             complement(9553..10890)
                     /locus_tag="VFO53_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HET8876667.1"
                     /translation="MTRFQAAARACLVAALAGAAPAGAEDLMDIYREAQRSDPALAAA
                     QANWTATQERVPQARAGLLPNVTLSASTNANYFGTNLDSDPHVSVNRGFGLGGLTVSA
                     AQPLYRYANTVAYSQAVQQVRQADYTLESAKQDLILRVATAYFDVLLAQFNVGLAESQ
                     KAAVSEQLAQAKRNFEVGVSTITDTNEAQAKYDSIVAQEISARNDLENRRTALRAIIG
                     RFPTDLKRLGPGFEPVLPSPNTLDAWVDRALADNLAVRVAQYNADIATLEIERQRAGR
                     LPTVDLVASANAQGFNASVNTNAAGEQHQVAIGLALTVPLYQGGFVDSKVREALALQD
                     NARQNLEVARRNALFGAQTGFSGVTSAAASVKAFEQALRSAQSAYESNKTGQEVGVRT
                     NLDVLNTQQNVFQARHDLAQAYFNYLLGVLRLKSAVGTLNEQDLEDVNRRLAG"
     gene            complement(10982..11641)
                     /locus_tag="VFO53_10105"
     CDS             complement(10982..11641)
                     /locus_tag="VFO53_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HET8876668.1"
                     /translation="MTRFDYEQARLNMIEQQIRTWEVLDVRVLDLLSAVRREEFVPPA
                     YRTLAFADLELPLPNGFRMWTPKMEARVLQELMVQSTDRVLEIGTGSGYLTALLASEA
                     GEVTSVEIDPATAADAHAKLARHGLANARVEVGDGARGFGNDTYDVIVLTGSTPLLPA
                     RFCEQLAPGGRIFAVVGEEPAMRARLVRLEAPGAAIATDLFETVIAPLVNAAAPARFE
                     F"
     gene            complement(11755..12912)
                     /locus_tag="VFO53_10110"
     CDS             complement(11755..12912)
                     /locus_tag="VFO53_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876669.1"
                     /translation="MPDPRRAARPRLSFARAYAIALKEFVQMRRDRLTFAMIVGIPIA
                     QVLLFGFVINTDPKALPTAVVDYDRTDLTRSIASTLSNTGYFSIVAAPRDADAADAML
                     ARGEIQFAIVFPSGFSRRLLRGERPSLLLAADATDPAATGNALAALTQAGTNALVHDL
                     TGPLARLAAQEPPFALDVQRRYNPEGVTQYNIVPGLLAVILQMTMVMMTAFAITRERE
                     RGTYEGLLATPVLPLEVMAGKIAPYIVVGLIQSAIILLVARYLFDVPMIGSLWLLGAT
                     MMLYIASLLGLGYAISTMAANQLQAMQMTFFFFLPTMLLSGFMFPFRGMPQWAQWLGE
                     LFPATHFLRIVRGILLKGNVGADVLSHVWPIALFMAAVTAIAMWRYRQTLD"
     gene            complement(12905..13867)
                     /locus_tag="VFO53_10115"
     CDS             complement(12905..13867)
                     /locus_tag="VFO53_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876670.1"
                     /translation="MPLTAEKSHRELAIDVRGLTKRYGGRAVVDDLAIRVGRGLIFGF
                     LGPNGSGKTTTIRMLCGLLTPDAGEGTCLGHDLRREAAAIKREVGYMTQRFSLYEDLS
                     IEENLDFVARMYAVADRRRAIDDTLARLGLSERRRQLAGTLSGGWKQRLALAACLLHG
                     PQLLLLDEPTAGVDPKARRDFWEEIHALAADGITVLVSTHYMDEAERCHRLAYINLGK
                     LLVEGTPDEVIARSQLHTWLVALAAGGDERALARLARELRDTRGVDSVIPFGLALRVS
                     GRDAAALAAALAPVQARPEFAVGTTTTSLEDVFVNLMQQAAPNA"
     gene            complement(13924..14880)
                     /locus_tag="VFO53_10120"
     CDS             complement(13924..14880)
                     /locus_tag="VFO53_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET8876671.1"
                     /translation="MTRSRLALAGVFALVACHDAAPGGYPGYVEGEFVRVAAPLSGTL
                     VTLAVERGTDVAKDAPLFTLESEQERAARAEAEARVRQAQATLANLGKARRPPEIAAA
                     RAQLAQAQASLVQSEADLARTQKLVADKFLPPQQRDQALAARNRDRARVAELTEQLRV
                     ANLPARSDEIAAAAAEVKAATDALAQAQWRVDQKLQTAPAAGLVSDTLYRPGEWVPAG
                     APVVSLLPPGNVKIRFYVPETLVGTLRLGGSVSAHCDGCGADIAAKIRFIAPQAEFTP
                     PVIYSRQNRANLVFLVEAWPDTPNAALHPGLPVDVTLATAAR"
     gene            complement(15007..>17127)
                     /locus_tag="VFO53_10125"
     CDS             complement(15007..>17127)
                     /locus_tag="VFO53_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8876672.1"
                     /translation="VNRVKSTIVEGAILTVLIVFFFLASWRSTIITGLTLPIAVVATF
                     IALRAFGFTLNFLTLMALSLTIGLLIDDAIVVRENIVRHLGMGKDHATAAREGTDEIG
                     LAVMATTFAIVAVFVPIAFMNGLVGRFFFQFGVSVAVAVLVSLFVSFTLDPMLSSVWA
                     DPAATRFAHARWLGRVMESVERGVDALHRVYAVVLAWALSHRKSVLAIALGTFAASFL
                     IVPLVGTEFMPEVDEGFISLRLNTPVGSSLEYTNGKVLEVEDTLRQFREVALMMTTVG
                     TEDGRNYARVNLRLAEASDRRRSQKELERTIRAALRPIPGIELAFGFDRPIWFNLMGP
                     DPDTLSKLAEDFAARVSKVRGIVDLESSDKAANPALSIRLNNAAASDLGITVQQVGNT
                     IRPLIAGDTISYWLGPDGQNYEVNVQLSKDRRQLASDLANLHLTTNRRGPDGQMRMVP
                     LRQVADLVETTSPQIIKRQDLSRRIGIYANVEGRPAGDVGTDVQRIANEMSLPPGYRL
                     QAAGQQQDMQESFASAVAALGLAIVFIYLILASQFASFTQPIAIMASLPFSLIGVFAA
                     LLLTGTTLNIFSVIGLVMLMGLVTKNAILLVDFANRARRAGASLTDALLTAGQVRLRP
                     IVMTTAAMIGGMLPLALGLGDGGEQQAPMGRAIIGGVITSTLLTLIVVPVIYAYLDGW
                     TQRRIARRARRAAAHAAPVAAGDD"
BASE COUNT         2699 a         5857 c         5914 g         2657 t
ORIGIN      
        1 tggtcgatca ccgcctggca ttcgctcgga cttgccgaat agaagtgtga atcgccgaag
       61 cccggggtgc gataaaaccg gcacactgga ttcgtgcccg ccgcgggcgc tgcgtacgcg
      121 aggaagcgca ggccggttcg ctcccatccg gagatcgcgc ccgaatcgag ggcgtcgagc
      181 tcgaccggac tgatcgtgat gaagtagtga tcgagggtgc ggttgtagta ctcgacggcg
      241 acggcgctgc cgggcgttcc cttggagtcg ggcgtcaggt actggcgcgt caacgcgagc
      301 attccgtcga ggcgcgagta ctcgtcgatt cccgtcgggt tctggcacga cgcggcgcca
      361 cgccacagtc cgccgcgcac ctcgtagtag ccggcgttgt tgaacgtcag aagtcccgcg
      421 ccgctcgagc ccggctcggt cgttccgcgg ctgtattgca cctgcgcgaa gctcgatccg
      481 tcggtgtaga gctggtagcc ctgcgccgcg ccctggctcc atttcttgag atcaccctcg
      541 ggatggtgaa tcaccgacac cgttgcggcc gacggaatcg gctcggcgcg ccagccagag
      601 aatttcgcgc cggccggcgg cgcggcgttg agccgcacga gcgcccaatc ggagtcggga
      661 ctccgcgcga gcatcgcagc acccgccgtt tgctgcacgt aggcgggcac cgtgtcgttg
      721 ccgcatgcca gtgcctcgaa gaaccagtag gtgttcaacg tgcgcgccgc catcgcggaa
      781 tcgaggcaat ggttggccga gaacaggtac ggcgtgttgc tcgccagcga atcgttcagg
      841 agctgaccgg tgcaaagata cgtgaagccg tcctcccgcg tgaactcgag cgcggcgacg
      901 gctttcgtgc tgtcgacgaa cgcactcgat tgcggcgaca cgcaggcgac gtcgatctcg
      961 cacgcgcccg acgtgccgat gtcctgcacg gcctttgcat cgagcttgcc gatcgacgcg
     1021 ggtgcgacga cctggtgcga aatgcgcgcg atcgtcagca gcgcgtcgga cgtgctcgtg
     1081 cctgcgtcgg cgtggatctc gatggttgcc gtatcgccgt cgagcaccgg cgaccagaag
     1141 acaccgaaac gctgcgtgtc gccggcgatc gcatttgcgg gaatcggccc gaatacctgc
     1201 gcacgcgcgc cgttgcctgc gaagcgcagg ctcacgccgg tgtccatcgc gggcaactgc
     1261 agcgcgacgc gcagcgctgc cgcaccgggc gacacgacgt cgatctttgc ggcgcggctg
     1321 ccgtcgggaa gcgacaccca ggcgaggccg gcaagcgaga ttgccgagct gcccgccggc
     1381 ggattgcgcg gaaacgcgag cgcgagcgga cccttgagat taccggcctt cgcggtgccg
     1441 cttgccgcgg acgcattgcg cgccttgagc gtcgcccgct cggccggcgt cggctcgggc
     1501 agcgagacac gcaacccgcg cgccgtggcg ggcagcacga gttgcgcttc tgcggacttt
     1561 tgcgtcgcgc ccggccgcag gtcggcgggg ggcacggcag tcaccggcac gtcggcctgc
     1621 accggcagcg cgagcgcaac cgcaacgatt gcgagcgcgg cgcgaagcgg cacacgcagg
     1681 aaagctttgg tcattcgaca ctccctcaag attcatgcgt cgccgggaag cgacggctgg
     1741 gtggctgctc taacgctgtt cgcgttgcaa atgccgagcc atcgacgggc ggtcaatgga
     1801 ccgctgcccc ggatggcggt tccgcatcgg cgcgcaggca ttcctgtaac agagtttgcc
     1861 agtcgggcag cgcgaagccg aacgtgcgcg caaagcgcgt cgcatcgagc acgccatagg
     1921 cgggacggcg cgccggtgcg ggatagtcga cgctggcaat gggcgtgatc tcgacgccgc
     1981 acccctcgag gattgcgcgc gcgaagccat accacgtcgt gctcccggct gcgctcaggt
     2041 gataaaggcc ggcccggtcg gcgagatgct cgcccgcctc gccgatcatg cgcgacgttg
     2101 cacgcgcaat ggaacggctc cagttcggca cgccggtctg gtcggcgacg acgcgcagat
     2161 ggctgcgccg ggcggcgaga ttttgcatcg tcaccaggaa attctgtccg tgccgcccgt
     2221 agacccagct cgtgcgcagc gtgatcgcga acgctcccga cgcgccgatc gcctgctcgc
     2281 ccgcgagctt gctctcgccg tacgcgttga tcggctgcgt tggcgccgac tcgtcgtacg
     2341 gcgccgtctg gcggccatcg aaaacataat cggtggaata gtggatgagc gtcgcgtgcg
     2401 cgcgccgcgc ctcgtcggca aggatgcccg gtgcgcgtgc attgatcgca agcgccgtct
     2461 cgcgctcggt ctcggcgcga tcgacgccgg tgtacgcggc cgcattgacg atgatctgcg
     2521 gacggacgcg gcgcacggcc tcgacgatcg ccgccggatc ggcgagatcg agcgtcgagc
     2581 ggtcgcacgc gacgacgtcg cccaccgcgg gcagcgcggc cgcgagctcg cgtccgagct
     2641 gcccgcgagc gcccgtcagc aggatcgtcg cacgcgtgcg cgccatccgc acctcacgca
     2701 gtcacgcata ggcttccgcc gccgcgaacg ggacaccggc cgcatccttc gccgcaagcg
     2761 tcggcgtgac ttgcgcgggc cacgcgatac cgagcgctgc gtcgttccac agcaacgtgc
     2821 gctcgtgcgc cggataccag taatcggacg tcttgtagag aaattccgca gcgtccgaca
     2881 gcacgacgaa gccgtgcgcg aagcccggcg gaatgaacag catgcgccgg ttctgcgaag
     2941 agagcgtcac ggcgacgtgg cggccgaagt gcggcgacga gcggcgcaga tcgacggcga
     3001 cgtcgaacac ctcgcccgcg gtcacgcgca cgagcttggc ctgcgcgtgc tcgatctggt
     3061 agtgcagacc gcgcaacacg ccatggcgcg agcgcgaatg attgtcctgc acgaaatcgg
     3121 cgtcgatgcc ggcttcggtg aatgcgcgct tgttgtagct ctcgaagaaa aagccgcgcg
     3181 cgtcgtcgaa cacgcgcggc tcgatgagca gcacgtcggg cagcgacgtc ggggacactc
     3241 gcatcaggag agcggcggct cttcgagaag ccgcagcagg tagcggccat agccgttctt
     3301 cgccatcgac gcaccgagct tctcgagctg cgccgcgtcg atgtagcgca tccggtaggc
     3361 aatctcctcc gggcaggcga tcttgaggcc ctggcgccgc tcgatcgtct cgatgtactg
     3421 cgacgcctcg agcagcgatc catgcgtgcc cgtgtcgagc caggccatcc cgcgtcccat
     3481 cacttcgcac gcgagctcgt cccatgcaag gtactgccgg ttgacgtcgg tgatttcgaa
     3541 ttcgccgcgc gccgacggcg cgaggccggc ggcaacgtcc aggacgcgat tgtcgtagaa
     3601 gtagagaccg gtcaccgcgt agcgcgattt cggctcgctc ggcttttcct cgaggctctt
     3661 cacgcggccg gtaacgtcga gctcggctac gccgtaacgc tccggatcgg cgacgggata
     3721 gacgaagatc gtcgccccgg ccgcacaagc gctcgcgcgc tcgagctgag gctgcagatc
     3781 gtggccgtag aaaatgttgt cgccgagcac gagcgccgac gcgtcgcgcc cgacgaattc
     3841 gcggccgatg acgaacgcct gcgcaagacc gtcgggcgac ggctgcaccg catacgagat
     3901 cgacaatccg aggcgcgtgc cgtcgccgag gagctgcgcg aagcgcggcg tgtcgtcggg
     3961 cgtggagatc agcaggatgt cgcgaatgcc ggcgagcatc agcgtcgaca gcgggtagta
     4021 gatcatcggc ttgtcgtaca ccggcagcag ctgcttcgac accacctgcg tgaccgggta
     4081 gagacgcgtg ccggagccgc cggcgaggat gatgcccttt ctatgcatat tgcaggtcca
     4141 cccagcggcg atactcggcg ttctgcacgc tcgcaagcca ttcgccgttg ccgagatacc
     4201 agtcgacggt gcgctgcatc cccgattcga acgtctccgc cggcgcccag ccgagctcgt
     4261 cgcggatctt cgtcgcatcg atcgcgtagc gccggtcgtg tccgggacga tcggtcacga
     4321 acgtcaccag ccgctcgcaa tcgcgcccgg gacgccgcgc ctgcacgagc cggcacacgc
     4381 tgcgcacgac atcgatgttg gccatctccg cgctgccgcc gacgttgtac gtctcgcccg
     4441 gccgcccctt gtcgagcacg gtgcgaatgg cgctgcagtg atcggcgaca tagagccagt
     4501 cgcgcacgtt cgttccgtcg ccatacaccg gaagtgcttt gcccgacagc gcgttgacga
     4561 tcatcagcgg aatgagcttc tcgggaaact ggcgcgggcc gtagttgttc gagcagttcg
     4621 tggtgagcgt cggcagcccg tacgtatggt ggtacgcgcg cacgaggtga tcggacgatg
     4681 ccttcgacgc cgcatacggg ctgttcggcg cgtacggcgt cgtttccgag aaggccgggt
     4741 cgccgggccc gagcgatccg tagacttcgt cggtcgacac gtgcaggaag cgaaacgcgt
     4801 tgcgcggctg cgccggcaga tcgctccacc atgcgcgcac gcattcgagc agcgcgaacg
     4861 tgccgacgac gttggtttgc acgaacgcgg ccggtccgtg gatcgagcgg tcgacgtgcg
     4921 tctcggccgc gaagttcacg attgcgcgcg gacgatgccg ctcgagaagc gaacgcacga
     4981 gctcggtgtc gccgatatcg ccgcgaacga acacgtgctc gccgttggcc gcgagcggcg
     5041 cgagcgtgcg caggttgccg gcgtacgtga gcttgtcgag attgacgagc ggctcggcgg
     5101 cctgcgcaaa ccagtcgaga acgaagttgg cgccgatgaa gccggcgccg ccggtaacga
     5161 ggatcatgtc ggaagggacg cagtcagcgc gaccgagttt agcatcggct gggtaacgac
     5221 ccccacgctc acgctgcccc cagagggggc gctattcgcg gcttgggacg gcccggcgcc
     5281 gcgatacgtc ccccacgctc gcgctgcccc agcgggggcg ctattcgcgg cttgggacgg
     5341 acggcgctcc aacgcggtag tagccacgat accagtcgac gaagcgcgcg aggccttcgt
     5401 cgagcggcgt atgcggcgca aagccggtcg cgcgtgcgag acgctctatc gacgcatacg
     5461 ttacgcgcac gtcgcccggc tgcatcgggc gcaggttgcg gatcgcacgg cgtccgagca
     5521 gccgctcgag cgtcgcaacg aagcggtcga gctcgacggc ttcgtgattg ccgatgttgt
     5581 agaccgcata cggcgcgccg tcgccatccg ccgaagggac cgttgcgagc acgcgcacga
     5641 cgccctcgac gatgtcgtcg atataggtga agtcacgctg catgcgcccc gcgttgaaca
     5701 cgtcgatagg cacgccttcg aggatcgcct tcgcgaagag catcggcgcc tggtcgggac
     5761 ggccccacgg cccgtacacg gtgaagaagc gcaggcccgt ggtgggcagc cggtagagat
     5821 ggctgtagct gtgcgccatc agctcgttcg ccttcttcgt cgcggcgtaa aggctgaccg
     5881 ggtgatcgac cggctggtcc tcggaaaacg gcagcacgtg attcgcgcca tacaccgacg
     5941 acgacgacgc atacacgagg tggcgaacgc tcgcgagccg gcagccctcg agcacgtgcg
     6001 cgaatgcaac gaggttgttg cgcacgtagg cctcgggatt cacgagcgag tagcgcacgc
     6061 ccggctgcgc cgcgagatgc acgacggcat cgaagcggcg cgcgaagagc gccgccgccg
     6121 cggccgcttc ggcaagatcg atttcctcga atgcaaagcc cgcgaatgca cgcagctcgt
     6181 tcacgcgcgc atgcttcagc gccacgtcgt agtacgcgtc caggctgtcg acgccggtca
     6241 ccgccgcacc gttgtcgagc aaggcgcgcg cgacgtgcat gccgatgaat ccggccgccc
     6301 ccgtgacgag cagccgctgc cccgcgggcg catcgcgcgt catcgcatgc gcttcgctgt
     6361 ttgccggatg cggcggcacg acgccgccac tcatcatccg cgccccgcgc gatcgagcgc
     6421 gcgcagcttc gcatgcttga ggaagctcgc aaatgccccg atggcgatgt gcgcaaagcc
     6481 ggccgcgccg tcgaggaagc cgagcttcag cacgtagaag cgcaggaacc gcgcaagcgg
     6541 cgccgcggcc atcgcgaacg ccgagctgcg ccgcccgctc gcgtgcagtg cgtcggcctg
     6601 cagcgtggtg taccggttct gcttcgcgat gtagcgctcg agcgactcgg ccgacgcgtg
     6661 cagcaggtcg ccgtcgagcc ttcccacggg tccctcggca atgacatgct cgtggaccgg
     6721 gtcgtcgctc cagcgcgcac gccgccggtc gaaaaggcgc gtggtccagt cgggatagcc
     6781 ttcgccgtgc gcgagccagc gcccgagaaa gcgattgcgg cgtgccagcg catacgccgc
     6841 gtgcggcggc tcgccggatg caaagagcgt cacgatcgac tcgcgcagcg ccgccgacaa
     6901 gcgctcgtcg gcgtcgaggc acagcaccca gtcgtgcacg gcctcctcga cggcgaactg
     6961 cttctgccgc ccgaacccga ggaacgcccg cgtgatcacg cgggcccccg tgcggcgcgc
     7021 ggtttctacg gtatgatcgc cgctccccga atcgacgacg agcgcgtcgc tcgcaaacgg
     7081 cgccgacagc aggcaatcgg cgatatcggc cgcggcgtcg cgcgtgatca cgaccagcga
     7141 aacgggaagc ggctcggcca tgcgcatttc gaatcgccgg cgtgactcat gccgcggcga
     7201 ttcgacgatc ctcacgtctt ctcgagatgg cgcgattcta cgttgtttga taccgacgac
     7261 cgcgtatccg catgagcggc gaagggtcct cggtgctcgt cgtccgcccg tcgtcgctcg
     7321 gtgacatcgt gtatgcgctg gcggccgccg ccgacatccg tcgcgcacgg cccgggcttg
     7381 cgatcgactg ggtgagcgag cccggcttcg cgccgctcgt cgcactgtgc cctgatgttc
     7441 gccgcgtgat cccgttcggc ctgcgccgct ggcgccgcac cccgctcgcc aggaccacat
     7501 ggcgcgatat ctgcgcattt cgcgccgacc tgcggcgcac gcgttacctc gcgatcctcg
     7561 atctgcagga gcaggtcaag ggcgcgttga tcgcgcgcgc agcacgcggc gtacgccacg
     7621 gcttcgatcg cgcgagcatc cgcgagccgc ttgcaacgct cggcgacgac attcatcacc
     7681 gcgtgccgcg cgagcttcac ttcctgacgc gttgccgccg gctcgcggcc gcggcgctcg
     7741 gatacgcgaa cgtggaaccg ccgcgctgga acctgcgtgc gccaacgatt gcgccggcga
     7801 tgcccgatcg accctacgcg gtcgtgctgc acggcaccag ccgcgacgac aagctctggc
     7861 ccgaaacgca ttggcgcgcg gtgctcgaca tgctcgagcg cgcggggatc gccaccgtgc
     7921 tgccctgggg cagcgatgcc gaggaagcgc gcagccgccg cctcgcccac gggtttgcgc
     7981 acgcggtcgt tccaccgtgg ctgtcgctgc cggaagcggc cgcgatgctc gcgaaggccg
     8041 cgctcgcgat cggcgtggac acggggttca cgcatcttgc ggccgcgctc ggaacgccga
     8101 gcatcgcgat cttcggcgcc accgatcctg ggcgccacgg tgtcgcgtgc gccggtgcgc
     8161 acgcgcagga tctgggcgat gcgggcgcgc cgccggcacc cgatgccgtg ctggcggcgg
     8221 ccggtgagat gctgcgccgc gcgccttgct gttgacgcgc atggcagcac gtctcgcata
     8281 cacgctgctg tggtggctgg cgctgccgtt ccttccgctg cgcctgtggt ggcgcggccg
     8341 gcgcgaggct tcgtatcggt tgcacatcgg cgagcgcttc ggccgctatg cgggtgccgc
     8401 gcgcgccgtg ggcaacgacg tcgtgtggat ccacgcggtt tccgtgggcg aaacgcgcgc
     8461 cgtcgtcccc ttggtcgagc gcctcgaacg cgacaatccg cggcgcacga tcctgctcac
     8521 gcacatgacg gcgaccggcc gcgacacggg gcgtgccctt ttcggcaacc gcgtcgtcca
     8581 gacgtggctc ccctacgacc ttccgttcgc cgtcgaccgt tttctcgcgc acttccatcc
     8641 gcgcgcgggc ctgctggtcg agacggagct ctggccgaac ctcgccgcgg ccgcagcgcg
     8701 aaggcgcgtg cccttgtttc tgatcaacgc gcggctgtcg gcgcgttcgg cgcgcgggta
     8761 tgcgcgcatc ggcccgctcg tgcgtccgtt gctgcgctcg ctctcgggcg tcgccgcaca
     8821 gacggacgac gacgcggcgc gattgcgcgc gctcggcgcc cgcgatgtcg cggtcaccgg
     8881 caacctcaag ttcgacgccg acatcgcacc cgcgatgcgc gagcgcgggc gcattttccg
     8941 tgaccggttc ggcatcgacc ggccggtcgt cgtgcttgcc tcgacgcgcg ccggcgagga
     9001 agcgctgctg ctcgacgcgc tcgcgcgcgc tgcattgccc ccacatgcgc tcgtcgtgat
     9061 cgttccgcgg catccgcaac gcttcgacga cgttgccgcg atgcttgcgt cgcgggcgca
     9121 gcacttcgta cgccgcagcg acgagtgcgc cgtgccgcgc gacgtcgcga tcgtcctcgg
     9181 cgattcgatg ggcgagatgc tcgcgtacta cgccgcggcc gacgtcgcgt tcgtcggcgg
     9241 cagcctgctg ccgctcggcg ggcagaacct gatcgagccg atcgcggccg gcgttccggc
     9301 gctggtgggt ccgcacacgt tcaatttcgc gcaggcggca cgtgctgcga tcgcagcagg
     9361 tgctgcacgg cgcgtcgaag acgcggatca gatgtggaag gtggcggcgc aattgctcgc
     9421 cgacacggca tcgcgcgagc ggatgtgcgc caacgcacga tcgttcatcg ccgcgcatcg
     9481 cggcgccgcg gatcggctgt ggacgtggct tgcgccgcgg atcgagcagc gccctcgacg
     9541 atcctgaccc gctcagcccg cgagccgcct gttgacgtcc tcgagatcct gctcgttcag
     9601 cgtgccgacg gcggacttca ggcgcagcac gccaagcagg tagttgaaat aggcctgcgc
     9661 gaggtcgtga cgcgcctgga acacgttctg ctgcgtgttc agcacgtcga gattcgtgcg
     9721 cacgcccact tcctggccgg tcttgttcga ctcgtacgcg ctctgcgccg agcgcagcgc
     9781 ctgctcgaac gccttcaccg acgcggccgc gctggtgacg ccgctgaagc ccgtctgcgc
     9841 gccgaacagc gcgttgcgcc gcgcgacctc caggttctgg cgcgcgttgt cctgcagcgc
     9901 gagcgcctcg cgcaccttcg aatcgacgaa accgccctgg tacaggggaa cggtcagcgc
     9961 gagcccgatc gccacctggt gctgctcgcc ggcggcattg gtgttcacgg aggcgttgaa
    10021 gccctgcgcg ttcgcgctcg caacgagatc gacggtggga aggcggccgg cgcgctggcg
    10081 ctcgatctcc agcgtcgcga tgtccgcgtt gtactgggcg acgcgtacgg caaggttgtc
    10141 ggcaagcgcg cgatcgaccc aggcgtcgag cgtgttcggc gacggcagca ccggctcgaa
    10201 gcccggcccg agccgcttca agtcggtggg aaagcgtccg atgatcgcgc gcaatgccgt
    10261 gcgccggttc tcgaggtcgt tgcgcgcgct gatctcctgc gcaacgatcg agtcgtactt
    10321 cgcctgcgcc tcgttggtgt cggtgatcgt cgaaactccg acctcgaagt tgcgcttcgc
    10381 ctgcgccagc tgctcggaaa ccgccgcctt ctggctctcg gcgagcccga cgttgaattg
    10441 cgccagcagc acgtcgaaat acgcggtcgc cacgcgcagg ataaggtcct gcttcgccga
    10501 ttcgagcgtg tagtcggcct ggcgcacttg ctggactgcc tggctgtatg caacggtgtt
    10561 cgcgtaacga taaagcggct gcgccgcgga cacggtgagc ccgcccaagc cgaagccgcg
    10621 gttcacgctc acgtgcggat cgctgtcgag attcgtgccg aaatagttcg cgttcgtgct
    10681 ggccgacagc gtcacgttgg gcagcaatcc ggcgcgcgcc tgcggcacgc gctcctgcgt
    10741 cgccgtccag ttcgcctgtg ccgcggcgag cgccgggtcg ctccgctgcg cctcgcgata
    10801 gatgtccatg aggtcctcgg cgcccgccgg cgccgcgccg gcgagtgcgg cgacgaggca
    10861 tgcgcgagcg gcggcttgga aacgcgtcat tgtcgaaaaa tccgtagggc gcgcgggcca
    10921 gggcgcgagg aggccttcac cgcgtgtgcg atcgcctgca gcgcgcaccg ggcggcgatg
    10981 atcagaattc gaaccgcgcg ggtgccgcgg cgttgacgag cggtgcgatc acggtctcga
    11041 acagatcggt cgcgatcgcc gcacccggcg cttccaggcg gacgaggcgc gcgcgcatcg
    11101 cgggctcctc gccgacgacc gcgaaaatgc gtccgccagg cgcgagctgc tcgcagaaac
    11161 gcgcgggcaa aagcggtgtc gagccggtga gcacgatcac atcgtacgtg tcgttgccga
    11221 agccgcgcgc gccatcgccg acctcgacac gagcgttcgc caggccgtgc cgagcgagct
    11281 tcgcgtgcgc gtcggcggcg gtggcgggat cgatctcgac gctcgtcacc tcgcccgcct
    11341 cgctcgcgag cagcgccgtc aggtaaccgc tgccggtgcc gatctcgagc acgcgatcgg
    11401 tcgactgcac catcagctcc tgcagcacgc gcgcttccat cttcggcgtc cacatgcgaa
    11461 agccgttcgg cagcggcagt tcgaggtcgg cgaacgcaag cgtgcggtag gcgggcggca
    11521 cgaactcctc gcgccgcacc gcggacagga ggtcgagcac gcgcacgtcg agcacctccc
    11581 aggtccgtat ctgctgctcg atcatgttga gccgcgcctg ctcgtaatcg aatcgggtca
    11641 tcgcgtggtc gcgggttgtg cggaacggga ggagtcatcg gcgctgaaac atgcggtggc
    11701 cgagacgacg tcgtggacgt gaggcgcggg gcattatagc aaggcggcaa gccgtcaatc
    11761 gagcgtctgc cggtagcgcc acatcgcgat cgccgtcacg gcggccatga aaagcgcaat
    11821 gggccagaca tgcgacagga cgtcggcgcc gacgttgccc ttgagcagga tgccgcgcac
    11881 gatgcgcagg aagtgggtcg ccgggaagag ctcgccgagc cactgcgccc actgcggcat
    11941 gccgcggaac gggaacatga atcccgacag cagcatcgtc ggcaaaaaga agaagaacgt
    12001 catctgcatc gcctgcaact ggttcgcggc catcgtcgag atcgcatagc cgagaccgag
    12061 cagcgacgcg atgtagagca tcatcgtggc gccgagcagc cacaggctgc cgatcatcgg
    12121 cacgtcgaaa aggtagcgcg ccacgagcag gatgatcgcc gactggatga gcccgacgac
    12181 gatgtacggc gcgatcttgc cggccatcac ctcgagcggc agcacgggcg tcgcgagcag
    12241 accctcgtag gtgccgcgct cgcgctcgcg ggtgatcgcg aatgcggtca tcatcaccat
    12301 cgtcatctgc aggatcaccg cgagcagccc ggggacgatg ttgtactgcg tcacgccttc
    12361 ggggttgtag cggcgctgca cgtcgagcgc gaacggcggc tcctgcgccg caagacgtgc
    12421 gagcggtccg gtcaggtcgt ggacgagggc attcgtcccc gcctgcgtca gtgccgccag
    12481 cgcattgccg gtggccgcag gatcggtcgc gtcggcagcg agcagcaatg aaggacgctc
    12541 gccgcgcagc agccggcgcg agaacccgga aggaaacacg atcgcgaact ggatctcgcc
    12601 gcgcgccagc atggcgtcgg ccgcatccgc gtcgcgcggt gccgccacga tggaaaagta
    12661 gccggtgttg gagagcgtgc tggcgatgct gcgcgtgagg tcggtccggt cgtagtcgac
    12721 gaccgctgtc ggcagcgcct tcggatcggt gttgatgacg aagccgaaca gcagcacctg
    12781 cgcgatcgga atgccgacga tcatcgcgaa cgtcagccgg tcgcggcgca tctgcacgaa
    12841 ttccttcagc gcgattgcgt aggcgcgcgc gaacgacagg cgcggccgcg cggcgcgccg
    12901 cggatcaggc attcggcgcg gcctgctgca tcaggttgac gaagacgtcc tcgagcgacg
    12961 tcgtcgtggt tccgacggcg aactcgggac gcgcctgcac cggcgcaagc gcggccgcga
    13021 gcgcggccgc gtcgcgtccc gacacgcgca gcgcgaggcc gaacggaatg acgctgtcga
    13081 cgccgcgcgt gtcgcgcagc tcccgcgcga gccgcgccag cgcgcgctcg tcgccacccg
    13141 cggcgagcgc cacgagccag gtgtgcagtt gcgaacgcgc gatgacttcg tccggcgtgc
    13201 cttcgacgag cagcttgccg agattgatgt acgcaagccg gtggcagcgc tccgcctcgt
    13261 ccatgtagtg cgtgctgacg agcaccgtga tgccgtcggc ggcgagtgcg tgaatctctt
    13321 cccagaaatc gcggcgggcc ttcgggtcga cgcccgcggt cggctcgtcg agcagcaaga
    13381 gctgcgggcc gtgcaacagg caggcggcga gtgccagccg ctgcttccat ccccccgaca
    13441 gcgtgccggc gagttgccgc cgccgctcgg acaggccgag tcgtgcgagc gtgtcgtcga
    13501 tcgcgcgccg ccggtcggcg accgcgtaca tgcgcgcgac gaagtcgagg ttctcctcga
    13561 tcgacaggtc ctcgtagaga ctgaagcgct gcgtcatgta gccgacctcg cgcttgatcg
    13621 ccgccgcttc gcggcgcagg tcgtgcccaa gacaggtgcc ctcgcccgcg tcgggtgtca
    13681 gcaagccgca cagcatgcgg atcgtcgtcg tcttgccgct gccgttgggt ccgaggaagc
    13741 cgaagatgag accgcgcccg acgcggatcg cgagatcgtc gacgaccgcg cggccgccat
    13801 agcgcttcgt gagcccgcgg acgtcgatcg caagttcgcg atggcttttt tcggccgtga
    13861 gcggcacagc ccctgccgtg cttgcaccgt cctccggcgc ctgcggcccg ggccccgctt
    13921 cgctcaccgg gcagcggtcg cgagcgtcac gtcgaccggc agtcccggat gcagtgccgc
    13981 gttcggcgta tcgggccagg cttcgaccag gaacacgaga ttggcgcgat tctggcggct
    14041 gtagatcacc ggcggcgtga attcggcctg cggcgcgatg aaccggatct tcgccgcaat
    14101 gtcggcgccg cagccgtcgc agtgcgcgct gacgcttccg ccaaggcgca gcgtgccgac
    14161 caatgtttcg ggaacgtaga agcgaatctt gacgttgccc ggaggcaaca gcgacacgac
    14221 gggcgcaccg gcagggaccc attcgccggg gcgatacagc gtgtcggaca cgagccccgc
    14281 ggccggcgcc gtctgcagct tctgatcgac gcgccactgc gcctgcgcga gcgcatcggt
    14341 ggcggctttc acctcggccg cggctgccgc gatctcgtcg ctgcgcgccg gcagattcgc
    14401 cacgcgcaac tgctcggtga gctcggcgac gcgcgcccgg tcgcgattgc gcgccgcgag
    14461 cgcctggtcg cgctgctgcg gcggcaggaa cttgtcggcg acgagcttct gcgttcgcgc
    14521 gaggtccgcc tcgctttgca cgagcgacgc ctgcgcctgc gcgagctgcg ctcgcgccgc
    14581 ggcgatttcc ggcggccgcc gcgccttccc gagattggcg agcgtggcct gtgcctggcg
    14641 cacccgcgcc tcggcctcgg cgcgcgcggc gcgctcctgc tcgctttcga gcgtgaacag
    14701 cggcgcatcc ttcgccacgt cggtgccgcg ctcgaccgcc agcgtcacga gcgtccccga
    14761 caggggcgcc gccacgcgca cgaattcgcc ttcgacgtag ccgggatagc cgcccggcgc
    14821 cgcgtcgtgg caggcgacga gcgcgaacac gcccgccagc gccagtcgcg acctcgtcat
    14881 cgggcaggct ccatccggcg atcataaccg gccccgcgca agggtcagcg gtgacgctcg
    14941 cgtgtcggct accaggagtc acgcgcgtgg aaaatcgccg ctgacgctgc tttggtcggc
    15001 ccgcgatcaa tcgtcgccgg ccgccacggg cgccgcatgt gccgccgcac gacgcgcgcg
    15061 ccgcgcgatc cgccgctgtg tccagccgtc gagatacgcg tagatcaccg ggacgacgat
    15121 cagcgtcagc agcgtcgacg tgatcacgcc gccgatgatc gcgcgtccca tcggcgcctg
    15181 ctgctcgccg ccgtcgccga ggccgagtgc cagcggcagc atgccgccga tcatcgcggc
    15241 ggtggtcatg acgatcgggc gcaaacgcac ctgcccggcg gtaaggagcg cgtcggtgag
    15301 gctcgcaccg gctcgccgcg cgcgattggc gaagtcgacg agcaggatcg cattcttcgt
    15361 gacgagcccc atcagcatga cgaggccgat gacggagaag atgttgagcg tcgtgcccgt
    15421 cagcagcagt gcggcgaaaa cgccgatcag cgagaacggc agcgacgcca tgatcgcgat
    15481 cggctgcgtg aagctcgcga actgcgacgc gaggatcagg tagatgaaca cgatcgcgag
    15541 gccgagcgcc gccaccgcgg acgcgaacga ctcctgcatg tcctgctgct gtcccgccgc
    15601 ctgcaaccga tagccgggcg gcagcgacat ctcgttcgcg atccgctgca cgtcggtgcc
    15661 gacgtcgccg gccgggcggc cctcgacgtt cgcgtagatg ccgatgcgcc gcgacaggtc
    15721 ctgccgcttg atgatctgcg ggctcgtcgt ctcgacgaga tcggcgacct ggcgcagcgg
    15781 caccatccgc atctgcccgt cggggccgcg ccggttcgtc gtcagatgca ggttcgcgag
    15841 gtcggacgcg agctggcgcc gatccttcga caactgcacg ttgacctcgt agttctgccc
    15901 gtcgggaccg agccagtagc tgatcgtgtc gccggcgatc agcggccgga tcgtgttgcc
    15961 cacctgctgg acggtgatgc cgagatcgga tgccgccgcg ttgttgaggc ggatcgacag
    16021 cgccggattc gccgccttgt ccgacgattc gagatcgacg atgccacgca ccttcgaaac
    16081 gcgcgcggcg aagtcttcgg cgagcttcga cagcgtgtcg ggatcgggcc ccatgaggtt
    16141 gaaccagatc ggccggtcga agccgaacgc gagctcgata cccgggatcg ggcgcagcgc
    16201 cgcgcggatc gtgcgctcga gctccttctg cgagcgccgg cgatcgctgg cctcggcgag
    16261 ccgcaggttg acccttgcat agttgcggcc atcctcggtg cccacggtgg tcatcatcag
    16321 cgcgacttcg cgaaactgcc gcagcgtgtc ctcgacctcg agcaccttgc cgttcgtgta
    16381 ctcgaggctc gagccgaccg gcgtgttgag ccgaagcgag atgaagccct cgtcgacctc
    16441 cggcatgaat tcggtgccca cgagcggcac gatcaggaag ctcgcggcga acgtgccaag
    16501 cgcaatcgcg agcaccgact tgcggtgcga cagcgcccat gcgagaacca ccgcatagac
    16561 gcggtgcagc gcgtcgacgc cgcgctcgac gctttccatc acgcggccga gccagcgcgc
    16621 atgcgcaaag cgcgtcgccg ccggatcggc ccataccgac gacagcatcg ggtcgagggt
    16681 aaagctcacg aacagcgaca cgagcaccgc cacggcgacg ctgacgccga actggaagaa
    16741 gaagcgcccg acgagcccgt tcatgaacgc gatcggcacg aacacggcga cgatcgcgaa
    16801 cgtcgtcgcc atcacggcaa gaccgatctc gtcggtgccc tcgcgcgccg ccgtcgcgtg
    16861 atccttgccc atcccgaggt ggcgcacgat gttctcgcgc acgacgatcg catcgtcgat
    16921 caggagaccg atggtgagcg acagcgccat cagcgtcagg aagttgagcg tgaagccgaa
    16981 tgcgcgcagc gcgatgaacg tcgccacgac ggcgatcggc agcgtcaggc cggtgatgat
    17041 cgtgctgcgc cagctcgcga ggaagaagaa cacgatcagc acggtgagga tcgcgccttc
    17101 gacgatcgtg ctcttcacgc gattgac
//
LOCUS       DASSVX010000231         5068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1176206, whole genome shotgun sequence.
ACCESSION   DASSVX010000231 DASSVX010000000
VERSION     DASSVX010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176206"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VFO53_10130"
     CDS             <1..963
                     /locus_tag="VFO53_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876673.1"
                     /translation="ISPDIWRKQLLDYASLGSHDKYAGAFTGEELRIIDQKLDRYMAR
                     KGARGEMPHLLIDRFRFDSFAPDSDEAGSNLLTRFGRILYLFFMITPPVSLVERAWNR
                     GLEVGRYKAVDDTLAHGVEAYSGMPELFFTWVARRDKRVHFEFLDNSVRLPAHPRTVA
                     FGWNDTMNILDVGCLLDVERYRRVDIGATGPASLYPDAQRLDPANNAGFLRQCIERFA
                     TVNFADQACGRVYLRVAGGAAACVDRDALAGAMADADTRAALAYVLPGVRLAALPAFE
                     RPAFVDDLAGAPRSTLGEWGWERAADDVRSGRDAAAHARDESRL"
     gene            complement(966..1226)
                     /locus_tag="VFO53_10135"
     CDS             complement(966..1226)
                     /locus_tag="VFO53_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET8876674.1"
                     /translation="MHLRQLTLLTRAYCHLCDDMRAALEPLAAEARTTIVELDVDADP
                     KLEARFGDRVPVLLDGDADGPELCHFRLDHERVRDALAITPK"
     gene            complement(1233..2696)
                     /locus_tag="VFO53_10140"
     CDS             complement(1233..2696)
                     /locus_tag="VFO53_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HET8876675.1"
                     /translation="MFTSLKWFTSSVLGMLVVLMPLVAPLPATAQGRQGALPDFTDLY
                     ERQGPAVVSIDVTQKARKQRFPDISEDDPFYEFFRRFGQVPRNGPGGGQQREFETQST
                     GSGFIISSDGYIVTNAHVVDDASEVTVRLSDKREFVAKVIGSDARTDVSLLKVDATEL
                     PRVAIGDPDKLRVGEWVVAIGKPFGLENTMTAGIVSAKGRDLPQENLVPFIQTDVAIN
                     PGNSGGPLFNMRGEVVGINSLIYSRTGGYMGLAFAVPIDIAMNAVTQLRDKGRVTRGR
                     IGVQIQEMTKETADAFGLKQANGALVNSVEKDGPAAKAGVQSGDIILKVEGRDVHSSN
                     DLPRIITAIRPGTKVTLNIWRKGTAKDIPVVIAEMKEDAAAANTPRRGANPAPKEKAK
                     PNRLGLVLSDLTDEQRKSIDLPSGGVLIEDVVGVVRGNVQPGDVIVAVIQRGAPIEVK
                     TAAQVNDYLAKLDKSAAVTLQVRRGDQTFYTTLKPGE"
     gene            complement(2737..3720)
                     /locus_tag="VFO53_10145"
     CDS             complement(2737..3720)
                     /locus_tag="VFO53_10145"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HET8876676.1"
                     /translation="MQHATRRSRLIPGRLAPAVFVLALAASAARADDAMQWLSRAAQA
                     ARQLDYAGTIVYQIGPRVESSRITHLNDAGHEFSKLVNLDGPAREVVRSDGELRCYYP
                     DAKLMRIEPGTFRNVFPSLLPEQQQSLSRYYDFRVLGEDRVGGRAVQVVVFDPKDGLR
                     YGHRFWSDAKTGLLLKARVVNERGDGIEQFAFSDLTINAAIDRSMVASSWPTVPPDWE
                     VLQNAAGDVVPQDTGWVVTRVPPGFAKIMEGYRKLRGRRERVAHLVFSDGLVSISVFV
                     EPLMASSSPAGVLRQGGLNVYSLKQDDYLITVMGETPGATVRQIAHSVMHR"
     gene            complement(3759..4271)
                     /locus_tag="VFO53_10150"
     CDS             complement(3759..4271)
                     /locus_tag="VFO53_10150"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HET8876677.1"
                     /translation="MNEQISRLMDGEIDATEMDAVCTTLRTDAAIATWNCYHVIGDAL
                     RSESAVTRNIASAISRRLADEPAIIARTAARPSGPAAWAWAAAASLAAMTVVGWTAYS
                     MVDTAPAGFAKAREAGTMSAAQMRPVTVLPADYLLAHQEYAPANALQGVGPYLRDVAA
                     STPVRAPAPQ"
     gene            complement(4271..4870)
                     /gene="rpoE"
                     /locus_tag="VFO53_10155"
     CDS             complement(4271..4870)
                     /gene="rpoE"
                     /locus_tag="VFO53_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HET8876678.1"
                     /translation="MGDREVDQELVLRAQRGDKRAFELLVVKYQRKLGRLLSRLVRDP
                     AEVEDVTQEAFIKAYRALPSFRGDSAFYTWLYRIAINTAKNYLVAMGRRAPTSTGFDH
                     EDAESFEDAEQLRDSATPEAELLGKQIAATVNRAMEELPDDLRTAITLREIEGLSYEE
                     IASVMNCPIGTVRSRIFRARESIAAELRPLLGTDENRRW"
BASE COUNT          798 a         1742 c         1656 g          872 t
ORIGIN      
        1 atcagtcccg acatctggcg caagcagctc ctcgattacg catcgctcgg atcgcacgac
       61 aagtatgcgg gcgcattcac cggcgaggag cttcggatca tcgaccagaa gctcgaccgc
      121 tacatggcgc gaaagggcgc acgcggcgag atgccgcatc ttctgatcga ccgcttccgc
      181 ttcgacagct tcgcgcccga ttccgacgaa gccggaagca acctgctgac gcgcttcggc
      241 cggattctct atctgttctt catgatcacg ccgccggtgt cactcgtcga gcgcgcatgg
      301 aatcgcggcc tggaggtcgg ccgctacaag gcggtcgacg acacgctcgc gcacggcgtc
      361 gaggcctact cgggcatgcc cgagctcttc ttcacctggg tcgcgcggcg cgacaagcgc
      421 gtgcacttcg aattcctcga caacagcgtg cggctcccgg cgcacccgcg taccgttgcg
      481 ttcggctgga acgacacgat gaacatcctc gacgtcggat gcctgctcga cgtcgagcgc
      541 tatcgccgcg tcgatatcgg cgcaaccggc ccggcgtcgc tctatccgga tgcgcaacgg
      601 ctcgatcccg cgaacaacgc cgggttcctg cgccagtgca tcgagcgctt tgccacggtc
      661 aacttcgccg accaggcgtg cggccgggtc tacctgcgcg tggcgggcgg cgcggctgca
      721 tgcgtcgatc gcgacgcgct tgccggcgcc atggccgacg ccgacacgcg cgcggcactc
      781 gcttacgtgc tgcccggcgt gcggcttgcg gcgctgcctg cattcgagcg gccggcattc
      841 gtcgacgacc tcgccggcgc gccgcgctcg acgctcggcg agtggggatg ggaaagggcc
      901 gcagacgacg ttcgatcggg tcgcgatgcc gcagcgcacg cgcgcgacga aagtcgtctc
      961 tgaccctact tcggtgtgat cgcgagtgcg tcgcgcacgc gctcgtgatc gagcctgaag
     1021 tggcatagct cgggcccgtc ggcgtcgccg tccagcagca ccgggacgcg atcgccgaat
     1081 cgggcctcga gcttgggatc ggcgtcgacg tcgagctcga cgatcgtcgt gcgcgcctcg
     1141 gccgcgagcg gctcgagtgc cgcgcgcatg tcgtcgcaca ggtgacagta cgcgcgcgtg
     1201 agcagcgtca gctgccgaag atgcacggcg gactactcgc ccggcttcag cgtcgtgtag
     1261 aacgtctggt cgccgcggcg cacctgcagc gtgaccgccg ccgacttgtc gagcttcgcg
     1321 aggtaatcgt tcacctgcgc cgcggtcttc acctcgatcg gcgccccgcg ctggatgacg
     1381 gcgacgatga catcgccggg ctgcacgttg ccgcgcacga cgccgaccac gtcctcgatc
     1441 agcacgccgc cgctcggcag atcgatcgac ttgcgctgct cgtcggtgag atcggacagc
     1501 acgaggccga gccggttggg cttcgccttt tccttcggcg caggattggc accgcggcgc
     1561 ggtgtgttgg ccgcggctgc gtcctccttc atttcggcga tcacgaccgg gatgtccttc
     1621 gccgtcccct tgcgccagat gttcagggtc accttcgttc ccgggcggat cgccgtgatg
     1681 atgcgcggca ggtcgttcga cgagtgcacg tcgcgtcctt cgaccttcag gatgatatcg
     1741 cccgactgca cgcccgcctt cgccgccgga ccgtccttct cgacgctgtt gacgagcgcg
     1801 ccattcgcct gcttcaagcc gaaggcgtcg gccgtttcct tcgtcatctc ctggatctgc
     1861 acgccgatgc gtccgcgcgt cacgcggccc ttgtcgcgaa gctgcgtcac ggcgttcatc
     1921 gcgatgtcga tcggcacggc gaacgcaagg cccatgtagc cgccggtgcg cgaatagatg
     1981 agcgaattga tgccgaccac ctcgcccctc atgttgaaga gcgggccgcc cgagttgccg
     2041 ggattgatcg cgacgtcggt ctggatgaac ggcaccaggt tttcctgcgg aaggtcgcga
     2101 cccttggcgc tcacgatgcc ggcggtcatc gtgttctcga ggccgaacgg cttgccgatc
     2161 gcgaccaccc attcgccgac gcgaagcttg tcgggatcgc cgatcgcgac gcgcggcagc
     2221 tcggtggcat cgaccttcaa cagcgacacg tcggtgcgcg catcgctgcc gatcaccttc
     2281 gcgacgaact cgcgcttgtc ggacaggcgc accgtcactt cgctggcgtc atcgacgaca
     2341 tgcgcattgg tgacgatata gccgtcggac gagatgatga agcccgagcc ggtggactgc
     2401 gtctcgaatt cgcgctgctg gcctccgccc ggcccgttgc gcggaacctg cccgaaacgg
     2461 cggaagaact cgtagaacgg gtcatcctcc gaaatgtcgg ggaagcgctg cttgcgtgcc
     2521 ttctgcgtga cgtcgatcga cacgacggcc ggcccctgcc gctcgtagag gtcggtgaag
     2581 tcgggcagcg cgccctggcg cccctgcgcc gtggcgggca gcggcgcgac gagcggcatc
     2641 agcacgacga gcatacccag cacggacgac gtgaaccatt tcaacgacgt gaacatgcgt
     2701 tcctcttcgg gtacggcgcc gacgcgcccg accccgttat cgatgcatca ccgagtgtgc
     2761 aatctggcgc acggttgccc ccggcgtctc gcccatgacg gtgatcaggt agtcgtcctg
     2821 cttcaggctg tagacgttga ggcccccctg gcgcaggacg ccggcgggtg acgacgacgc
     2881 catcaggggt tcgacgaaca cgctgatcga gaccaggccg tcggaaaata ccagatgcgc
     2941 gacgcgctcg cgacgtccgc gcaacttgcg atagccttcc atgatcttgg cgaagcccgg
     3001 cggcacgcgc gtcaccaccc agccggtgtc ctgcggcacg acgtcgcccg ccgcattctg
     3061 cagcacctcc cagtcgggag gaacggtcgg ccacgacgac gccaccatcg agcggtcgat
     3121 cgctgcgttg atcgtcaggt cgctgaaggc gaattgctcg atgccgtcac cgcgctcgtt
     3181 cacgacgcgc gccttcagca acagcccggt cttggcgtcg gaccagaagc gatggccgta
     3241 gcgcaacccg tccttcggat cgaacacgac cacctgcacc gcgcggccgc cgacgcggtc
     3301 ttcgccgagc acgcggaaat cgtaatagcg cgacagcgat tgctgctgct cgggaagcag
     3361 cgacgggaac acgttgcgaa acgtgccggg ctcgatgcgc atcagcttgg cgtcggggta
     3421 gtagcagcgc agttcgccgt cgcttcgcac gacctcgcgc gcgggcccgt cgaggttcac
     3481 gagcttcgag aattcgtgac cggcgtcgtt gagatgcgtg atccgcgacg attcgacccg
     3541 cggaccgatc tggtagacga tcgtgccggc gtaatcgagc tggcgcgcgg cctgcgccgc
     3601 gcgcgacagc cactgcatcg catcgtcggc ccgggccgcc gacgcggcga gcgcgagtac
     3661 aaatacggcc ggggcaagcc ggccgggaat gagtcgggaa cgacgtgttg cgtgctgcat
     3721 cttcgccgat ggcaagggtt atccgggacc gcggatcgtc attgcggagc cggagcacgc
     3781 accggcgtgc tggcggcaac gtcgcgcaga tagggaccca cgccctgcag cgcattggcc
     3841 ggcgcgtatt cctgatgcgc aagcaggtag tcggcgggca gcaccgtgac cggccgcatt
     3901 tgcgcggcac tcatcgtgcc tgcttcgcgc gccttcgcga agccggcggg cgccgtgtcg
     3961 accatcgagt acgccgtcca gccgacgacg gtcatcgcgg cgagcgaggc ggccgcggcc
     4021 catgcccatg ccgccggacc cgaggggcgt gcggccgttc gcgcgatgat cgcgggctcg
     4081 tccgcgagcc tgcgcgagat cgcggacgcg atgtttcgcg tcaccgcgct ttcgctgcgc
     4141 agcgcatcgc cgatcacgtg atagcagttc cacgtggcga tcgccgcgtc ggtccgaagc
     4201 gtcgtgcaga ccgcatccat ttcggtcgcg tcgatctcac catccatcag acgcgaaatt
     4261 tgctcgttca tcaccatctc cggttttcgt cggtgccgag cagcggccga agctcggccg
     4321 cgatcgactc tcgtgcgcga aagatgcgcg atcgcaccgt gccgatcggg cagttcatca
     4381 cgcttgcaat ttcctcgtaa ctcaacccct cgatctcgcg caacgtgatc gccgtgcgca
     4441 ggtcgtcggg caactcttcc atcgcgcgat tcaccgtcgc cgcgatctgc ttgccgagca
     4501 gctcggcctc gggcgtcgcc gaatcgcgca gctgttccgc atcctcgaag ctctccgcat
     4561 cctcgtggtc gaacccggtc gacgtcggcg cccgccggcc catcgcgacc aggtagttct
     4621 tggccgtgtt gatcgcgatg cgataaagcc atgtatagaa cgcgctatcc ccgcgaaagc
     4681 tcggcagtgc gcggtacgcc ttgatgaagg cttcctgcgt cacgtcctcg acctccgccg
     4741 ggtcgcgcac caaccgggac aggagtcgcc ccagcttgcg ctggtatttg acgaccagga
     4801 gctcgaacgc gcgcttgtca ccccgttgcg cgcgcagcac gagttcctga tcgacttcgc
     4861 gatcacccat ccgccggcag tccctgattc gcgtcgttgt tatgcccgcc gaacggcggg
     4921 tgcggctgcc tgcgatgccg agcggctgtc ccggagcggg aggcgcgaaa tggcacgtgg
     4981 cgcacgcttg gagcaacaga ccgttgcgcg ccgcccaagt tcagccgatc acgcatcggg
     5041 gaacgccgat agttgcgggc gaaaggcg
//
LOCUS       DASSVX010000232         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_492894, whole genome shotgun sequence.
ACCESSION   DASSVX010000232 DASSVX010000000
VERSION     DASSVX010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492894"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFO53_10160"
     CDS             complement(<1..513)
                     /locus_tag="VFO53_10160"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET8876679.1"
                     /translation="MTTGADDPRPAFSGAAADALIEHRLSGQQVYSGALLDVRRDQVR
                     LPDGGEAVREYIVHPGAVLMIPVLDDGGLIAVRQFRYPQNRAFMEFPAGKLDAGESPL
                     ATAERELVEEVGYSARQWTRLGVIHPVISYSTEAIEIYVGRFLWHVGAKLDPGEFLDV
                     LRCTQADLYAA"
     gene            complement(510..1451)
                     /gene="hemF"
                     /locus_tag="VFO53_10165"
     CDS             complement(510..1451)
                     /gene="hemF"
                     /locus_tag="VFO53_10165"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HET8876680.1"
                     /translation="MTADVRAIDAISGCEIADIRAYFTGLQATIVEALERIDGATFRR
                     DEWRRPAGGGGTSMSLEDGRVLERAGVLFSHVTGDALPPSATAHRPQLAGRAWEALGV
                     SLVLHPRNPFAPTVHLNVRYFVARPRGAAEAPVWWFGGGMDLTPVYGFVDDAQHFHRT
                     CRDALAPFGADLYPRFKRNCDAYFFLKHRQEARGVGGIFYDDFDEGGLVSAFAIARSV
                     GDHFLPAYLPILERRSDTPFGERERDFQAYRRARYVEFNLVYDRGTLFGLQSNGRTEA
                     ILLSMPPLARWRYEWAPAPGTPEAQLYSDFLPVREWL"
     gene            complement(1448..2722)
                     /gene="purD"
                     /locus_tag="VFO53_10170"
     CDS             complement(1448..2722)
                     /gene="purD"
                     /locus_tag="VFO53_10170"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET8876681.1"
                     /translation="MNVLIVGGGGREHALAWKVAQSPRVGKVFVAPGNAGTAIDPDLT
                     NVPIADIATLVAFAKREAIALTIVGPEGPLATGIVDAFTAAGLRIFGPTQAAARLESS
                     KDFAKAFMTRHRIPTAAYGTFTDAAAAHAFVTSRGAPIVVKADGLAAGKGVVVAMRED
                     EAHAAIDAMFAAGGGARVVIEEYLTGEEASFIVMADGTHALALASSQDHKRLLDGDTG
                     PNTGGMGAYSPAPVVTPTVHARIMREVIAPALSGMAAEGMPYTGFLYAGVMIDDKGAP
                     RVLEFNCRLGDPETQPIVARLRSDLTTLVDHALAGTLDRVEAEWDRRAALTVVLAAAG
                     YPDSPQRGATIEGLDRITTTAHPNVTVFHAGTQWTDGRIDVSGGRVLGVTALGDSLRQ
                     AQRAAYAAVDDIHFDGMQFRTDIGHRALKARP"
BASE COUNT          527 a          975 c          925 g          461 t
ORIGIN      
        1 ggccgcgtag agatcggctt gcgtgcagcg aaggacatcg aggaattcgc cgggatcgag
       61 ctttgcgccg acgtgccaca gaaaacgtcc gacgtagatc tcgatcgcct cggtcgaata
      121 cgaaatcacc ggatgaatga cgccaagccg cgtccactgc cgcgccgaat agcccacttc
      181 ctcgacgagc tcccgctcgg ccgttgcaag cggcgactcg ccggcgtcga gcttgccggc
      241 gggaaactcc atgaatgcgc ggttttgcgg atagcggaac tggcgtacgg caatcaatcc
      301 gccgtcatcg agaaccggaa tcatcaacac ggcacccgga tgcacgatgt attcacgcac
      361 ggcctcgcca ccatcgggca agcgaacctg atcgcggcga acatcgagca gcgcaccgga
      421 atagacctgc tgacccgaca aacgatgctc gatcaatgca tcggctgcag caccgctgaa
      481 cgcgggacgc ggatcatccg caccggtggt caaagccact ccctcacggg gagaaagtcg
      541 ctgtaaagct gcgcctccgg cgtgccgggc gccggcgccc actcatagcg ccatcgtgcg
      601 agcggcggca tcgacagcag gatcgcctcg gtgcggccat tcgattgcag tccgaacaac
      661 gttccacggt cgtagacgag gttgaattcg acgtagcgtg cgcgccggta agcctggaag
      721 tcgcgctcgc gttcgccgaa cggcgtatca ctgcgccgct cgagaatggg caggtacgcg
      781 ggcaggaagt gatccccgac gctacgcgca atcgcgaacg cgctcaccaa gccaccctcg
      841 tcgaagtcgt cgtagaagat gccgccgacg ccccgcgctt cctgccggtg cttcaggaag
      901 aaatacgcat cgcaattgcg cttgaagcgc ggatagagat cggcgccgaa aggcgccagt
      961 gcatcgcggc aggtgcgatg aaagtgttgc gcgtcgtcga cgaagccata caccggcgtc
     1021 agatccatgc cgccaccgaa ccaccatacc ggtgcttccg cggcgccgcg tggccgcgcg
     1081 acgaaatacc gaacgttgag atgaacggtt ggcgcaaacg gattgcgcgg atgcagcacc
     1141 agcgacacgc cgagtgcttc ccacgcacga ccggcaagct gcggccggtg cgccgttgcc
     1201 gatggcggca gtgcgtcacc ggtcacatgc gagaacagca cgccggcgcg ctcgagcaca
     1261 cggccgtcct cgagggacat cgacgttccg ccaccgcccg cggggcgccg ccactcgtcg
     1321 cggcgaaacg ttgcgccgtc gatgcgctcg agcgcctcaa cgatcgtcgc ctgcaagcca
     1381 gtgaagtacg cgcgaatatc agcgatctcg catcccgaga tcgcgtcgat ggcacgcaca
     1441 tccgcggtca tggacgcgcc ttcagcgcgc gatggccgat gtccgtgcgg aactgcatgc
     1501 cgtcgaaatg gatatcgtcg acagcggcgt acgcggcgcg ctgcgcctgt cgcagcgaat
     1561 cgccgagcgc cgtcacgccg agcacgcgac caccgctgac gtcgatgcgg ccgtcggtcc
     1621 actgcgtgcc ggcgtggaat acggtgacgt tcgggtgcgc ggttgtcgtg atccgatcga
     1681 gtccttctat cgttgcaccg cgctgcggcg agtcgggata accggccgcg gccagcacga
     1741 cggtcagcgc agcgcgccga tcccattcgg cttccacgcg atcgagggta ccggcgagcg
     1801 cgtggtcgac gagcgtcgtc aaatcggagc gcagacgcgc aacgatcggt tgcgtctcgg
     1861 ggtcgccgag cctgcaattg aactcgagga cgcgcggcgc gcctttgtcg tcgatcatca
     1921 cgcctgcgta aaggaaaccc gtgtacggca tgccttccgc cgccattcct gagagcgcgg
     1981 gcgcgatcac ctcgcgcatg atccgcgcgt gcaccgtggg tgtgaccaca ggcgcaggtg
     2041 aatacgcgcc catgccgccg gtattgggcc cggtgtcgcc gtcgagcagc cgcttgtgat
     2101 cctggctcga tgcgagagcc agcgcatgcg tgccgtcggc catcacgatg aagctcgctt
     2161 cctctccggt caggtactcc tcgatgacga cgcgtgcccc accgcctgct gcgaacatcg
     2221 catcgatcgc cgcgtgcgct tcgtcctcgc gcatcgcgac gacaacgccc ttccccgcgg
     2281 caagtccgtc ggccttgacg acgatgggcg caccgcgcga cgtgacgaat gcgtgcgcgg
     2341 cggccgcgtc ggtgaacgtg ccgtacgccg ccgtcggtat ccggtggcgc gtcatgaatg
     2401 ccttcgcaaa atccttcgag ctctcgaggc gcgcagccgc ttgcgtcggt ccgaagatgc
     2461 gtagccctgc cgcggtaaac gcatcgacga tgccggtggc gagcggacct tcgggaccga
     2521 cgatcgtcag tgcaatcgcc tcgcgcttcg cgaatgcgac gagcgtcgcg atatcggcga
     2581 tcggcacgtt tgtcaggtcc ggatcgatcg ccgtgccagc gttgccaggt gccacgaata
     2641 ccttgccgac gcgcggcgac tgcgcaacct tccacgcaag cgcgtgctcc cggccgccgc
     2701 cacccacgat caggacattc attcccgtcc gctgttcatc gcggtcgaag gttcagtgcc
     2761 gaaaatgccg tacgccggtg aaaaccatcg cgatgccgcg ctcgttcgcg gcggcgatca
     2821 cttcgtcgtc gcgaacgctg ccaccgggct ggatgacggc gatggcgcca ttgtccgcga
     2881 cgacatcg
//
LOCUS       DASSVX010000233         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_810041, whole genome shotgun sequence.
ACCESSION   DASSVX010000233 DASSVX010000000
VERSION     DASSVX010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810041"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFO53_10175"
     CDS             <1..475
                     /locus_tag="VFO53_10175"
                     /inference="COORDINATES: protein motif:HMM:NF040132.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876682.1"
                     /translation="PVVEFHNARDGQYFLTADPNEAALLDGGGLGPDWSRTGVSFAAF
                     ARDATDARAVPVCRFFGTPGVGPNSHFYTAYASECDAVRKDSHWIEEGVTFRAMLPDA
                     GRCADGDATVLRLWNPGTSVTETRHRYVVDASTAAAMQAAGWVLEGPVFCSPGNG"
     gene            487..1380
                     /locus_tag="VFO53_10180"
     CDS             487..1380
                     /locus_tag="VFO53_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HET8876683.1"
                     /translation="MSAPTLAGKLVVAISSRALFDLADSHRVYTDLGVESYHRYQIEH
                     EDELLEPGPAFALVTKLLRLNRLSKQYVEVILLSRNSADTGLRVFNAIKHYGLDITRA
                     AFTKGEPTSRYVPAFGAHLFLSADTDDVRRALEDGYAAATIFPSVFRNETDELRIAFD
                     GDAVLFSDEAERIYQANGLAAFAQSERDAAATPLSGGPFKEFLAALHRIQSDFPEDRS
                     PIRTALVTARSAPAHERVIRTLRAWNIRIDEALFLGGLDKGEFLRAFGADIFFDDQAR
                     HVESAALHVSAGHVPHGIANQ"
     gene            1469..1960
                     /locus_tag="VFO53_10185"
     CDS             1469..1960
                     /locus_tag="VFO53_10185"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET8876684.1"
                     /translation="MTRLVAIGIAVALMAMPASADDYKIGVMHVERIMRQSKPAREAQ
                     ARIEQEFKARDADITRKEQEVQAAAAQFAKDRVTLSPGDAANRERALDLRTRDVQRLR
                     QQFAEDLRARQFEELDKLKARLDQVLTQYAKDNHFDLILQDALFVGRAVDITDDVIKG
                     LEK"
     gene            complement(2058..3089)
                     /locus_tag="VFO53_10190"
     CDS             complement(2058..3089)
                     /locus_tag="VFO53_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8876685.1"
                     /translation="MERSVMEYRRLGKSGLEVSIVCLGTMMFGDRTGAREANEIVAHA
                     QGAGVNFIDTADVYASGESETITGAAIGAKRDRWILATKVGNAMKGDANGAPFTGGAS
                     RRWILAECDASLRRLRTDYIDIYYLHIDDAGTPLDETVAAMGTLIDSGKIRYFGVSNF
                     RGWRIAELVRRCERQHVAPPVVCQPYYNLLNRMPEVEVLAACDHYGIGVASYSPIARG
                     VLTGKYAGGVIPGDSRAARNDRRLMQTEFRSESFAIAQQLAGHAAKTGRTPVQFALAW
                     LWANRIVTSVIAGPRTLDQWRDYVAAIGTPWSAADEALVDSLVRPGHPSTPGYSDPQY
                     PICGRRLEN"
     gene            3214..>3761
                     /locus_tag="VFO53_10195"
     CDS             3214..>3761
                     /locus_tag="VFO53_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HET8876686.1"
                     /translation="MNVLFEDNGHLKAGTLLADHDASVQVEAVSGKRQKIKAAHVLLR
                     FADPSPAETMARAQQLVAELDPDFLWEVVPDDEFGFEELAREYYGAAPAPAETAAVAL
                     ALARAPTHFYRKGRGRFRKAPPEALKAALASLARKEREARDIAHWVAELQAHRLPPEL
                     AAKLDMLLYRPDRNAPEWKALNL"
BASE COUNT          603 a         1295 c         1258 g          605 t
ORIGIN      
        1 tccggtggtc gagttccaca acgcacgcga cggccagtac ttccttaccg ccgacccgaa
       61 cgaggcggcg ctgctcgacg gtggcggtct tggccccgac tggtcgcgca ccggcgtctc
      121 gttcgccgcg ttcgcgcgcg acgccaccga tgcacgcgcc gttcccgtat gccggttctt
      181 cggcacgccg ggtgtcggcc cgaactcgca tttctatacg gcgtacgcga gcgaatgcga
      241 cgcggtgcgc aaggattcgc actggatcga ggagggcgtc acgtttcgcg cgatgctgcc
      301 ggacgctggc cgctgcgccg acggcgacgc tacggttttg cggctgtgga acccgggaac
      361 gagcgtcacc gagacacggc atcgctacgt cgtcgacgcg tcgaccgccg ccgcaatgca
      421 ggctgctggc tgggtgctcg agggcccggt gttctgttcg cccggcaacg gctagtattc
      481 gtcaccatga gcgctcccac gcttgccggc aagctcgtcg tcgcgatcag ctcgcgcgcg
      541 ctgttcgatc tcgccgacag tcaccgcgtc tacaccgacc tcggtgtcga gagctatcac
      601 cgctaccaga tcgagcacga ggacgagctg ctcgagccgg gcccggcgtt tgcactggtc
      661 acgaagctgc tgcgcctcaa tcgcctgtcg aagcagtacg tcgaggtcat cctgctttcg
      721 cgcaacagcg ccgacaccgg gctgcgcgtg ttcaacgcga tcaagcatta cggtctcgac
      781 atcacgcgcg ccgccttcac gaagggcgag ccgacgtcgc gctacgtgcc cgcgttcggt
      841 gcgcacctgt tcctgtcggc cgacacggac gacgttcgtc gcgcgctgga ggacggctac
      901 gccgcggcga cgatcttccc gagcgtgttc cgcaacgaga ccgacgagct gcgcatcgca
      961 ttcgacggcg acgccgtgct gttctccgac gaggccgagc gcatctacca ggcgaacggg
     1021 ctcgccgcgt tcgcgcaaag cgagcgcgat gcggcggcca cgccgctctc gggcggcccg
     1081 ttcaaggagt tcctggccgc gctgcaccgg atccagtccg attttccgga ggaccgcagc
     1141 ccgatccgca cggcgctggt gacggcgcgc agcgccccgg cgcacgagcg cgtcatccgc
     1201 acgctgcgcg catggaacat ccgcatcgac gaagcgctgt tcctcggggg cctcgacaag
     1261 ggcgagttcc tgcgcgcgtt cggcgccgac atcttcttcg acgaccaggc gcggcatgtc
     1321 gaatcggcgg cgctgcacgt ctcggcgggg cacgtgccgc acgggatcgc gaaccaatga
     1381 gcgctgcaga gccgcctcaa ggcgcgaatt gcgccccctc ggggggcagc gcagcggcga
     1441 agccgcaagc gtggggggcc atacgagcat gacgagactc gtcgcgatcg gcatcgcggt
     1501 tgcgctgatg gcgatgcccg catccgccga cgactacaag atcggcgtca tgcacgtcga
     1561 gcggatcatg cgccagtcga aaccggcccg cgaggcgcag gcgcgcatcg agcaggaatt
     1621 caaggcgcgc gacgccgaca tcacgcgcaa ggagcaggag gtgcaggcgg cggctgcgca
     1681 gtttgcgaaa gaccgcgtga cgctatcccc gggcgacgcg gcaaaccgcg agcgcgcgct
     1741 ggacctgcgt acgcgcgacg tgcagcggct gcgccagcag ttcgccgagg atcttcgcgc
     1801 gcgtcagttc gaggagctcg acaagctgaa ggcgcgcctc gaccaggtgc tgacccagta
     1861 cgcgaaggac aaccatttcg acctgatcct gcaggatgcg ttgttcgtcg gccgcgcggt
     1921 agacatcacc gacgacgtga tcaagggcct cgagaagtag ggtcagagac gactttccga
     1981 gaagtagggt cagagacgac tttcccgtga cgattcggcc gacgaccccg cttgcgcccg
     2041 gaaagtcgtc tctgacccta attttccagg cgccgcccgc agatcggata ctgcgggtcg
     2101 ctgtacccgg gcgtcgatgg atggcccggg cggacgagcg agtcgacgag cgcctcgtcc
     2161 gcagcactcc acggcgtgcc gatcgccgcg acatagtcgc gccactggtc cagcgtgcgc
     2221 ggcccggcga tcaccgacgt cacgatgcgg ttggcccaga gccatgcgag tgcgaactgc
     2281 accggcgtcc gcccggtttt cgccgcgtgc ccggcgagtt gctgcgcgat ggcgaacgac
     2341 tcgctgcgaa actcggtctg catcaggcgc cggtcgttgc gcgccgcacg cgagtcgccg
     2401 ggaatgacgc cgccggcgta cttgccggtc agcacgccgc gcgcgatcgg cgaatacgat
     2461 gcgacgccga tgccgtagtg gtcgcatgcg gccagcacct cgacttccgg catgcggttc
     2521 aagaggttgt agtacggctg gcacacgacc ggcggcgcga cgtgctgccg ctcgcagcgg
     2581 cgcacgagct cggcgatgcg ccagccgcgg aaattcgaca cgccgaaata gcggatcttt
     2641 cccgaatcga tcagcgtgcc catcgccgcg accgtttcgt cgagtggcgt gcccgcgtcg
     2701 tcgatgtgca ggtagtagat gtcgatgtaa tcggtgcgaa ggcgccgcag gctcgcgtcg
     2761 cattcggcga gaatccagcg ccgcgatgcg ccgccggtga acggcgcgcc gttcgcatcg
     2821 cccttcatcg cgttgccgac cttcgtcgcc agaatccagc gatcgcgctt tgcgccgatc
     2881 gccgcgccgg tgatcgtttc cgattcgccg ctcgcgtaaa cgtcggccgt gtcgatgaag
     2941 ttgacccccg caccctgcgc gtgcgcgacg atctcgttcg cctcgcgcgc gccggtgcga
     3001 tcgccgaaca tcatcgtgcc gaggcagacg atcgaaacct cgagcccgct tttgcccagc
     3061 cttcggtatt ccatcacgct tcgctccatg gtttcgatcc tctgtcctgt gactcccgga
     3121 gctctgaatg gtacccggta taattacgtc tcccgtcaac gtcgcggccg gcttgtcgca
     3181 gctcgctttg cgcccgcgcg atttccgaat tccatgaatg tgctgttcga ggataacggc
     3241 catctgaagg ccggcacgct cctcgccgac cacgacgcat cggtgcaggt cgaggcggtc
     3301 tccggcaagc ggcagaagat caaggcggcc cacgtgctgc tgcgcttcgc cgacccgtcg
     3361 ccggcggaga cgatggcgcg cgcgcaacag ctcgtggcgg agctcgatcc ggatttcctc
     3421 tgggaagtag ttcccgacga cgagttcggc ttcgaggagc tcgcccgcga atactacggc
     3481 gcggcgcccg cgcccgccga aacggccgcc gtcgcgctcg cgcttgcccg cgcgccgacg
     3541 catttctatc gcaagggtcg cggccgcttc cgcaaggcgc cgcccgaggc gctgaaagcg
     3601 gcgctcgcgt cgctggcgcg caaggagcgc gaggcgcgcg acatcgcgca ctgggtcgcc
     3661 gagctgcagg cgcaccggct gccgcccgag ctggcggcga agctcgacat gctgctatac
     3721 cggccggaca ggaacgcgcc ggagtggaag gcgctgaacc t
//
LOCUS       DASSVX010000234         4201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1076719, whole genome shotgun sequence.
ACCESSION   DASSVX010000234 DASSVX010000000
VERSION     DASSVX010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076719"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VFO53_10200"
     CDS             complement(<1..1036)
                     /locus_tag="VFO53_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET8876687.1"
                     /translation="MAEFATENVRTVAWVGDGGSGKTSLVEALLTKAGAIAAAGSVER
                     GSTVSDFDPLEKTYQHSLRASLVHLETAGTRIHMIDTPGLPDFIGQSIGALDAVETAA
                     VVVNAATSVQMIGARMMEWAAQRKLCRLVVINRIDAENVDLPGVLEAVREAFGRECLP
                     INLPAGGASHVVDCFFNPAGESDFSSVAEAHRALVDQVVEVDEELMSLYLEQGEVAPE
                     QLHAPFEKALREGHLVPVCFVSARTGAGVSELLDVLVRLAPNPAEGNPPLFIKGEGDN
                     AVEFHSEPDPSKHVLAHVFKVVMDPFVGKLGVFRVHQGTVTRDTQLFVGDGRKPFKVG
                     HLFMLQGAKYI"
     gene            1212..2114
                     /locus_tag="VFO53_10205"
     CDS             1212..2114
                     /locus_tag="VFO53_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET8876688.1"
                     /translation="MPRSGLSRAGRRIAHDPLGFALQTLRAFRANQGLLLAGGVAYYT
                     LLSIVPLMILLVIILSHVVDAARLMSTLARYLEIVAPGQSGPLIDELRSFVAHREVIG
                     WTLAITLIFFSSLAFSVLENAMSVIFYHRVLTKRRPFVISILIPYLYILFLGIGLLVV
                     TLVAGALQSLALTTLDVLGHDWSLSGVSRTLLYVIGVFGEVLVLASIYFVMPVGRLSW
                     RHALIGGVSATVLWEISRHVLVWYLATLSQVQTVYGSLTTSIVVLLSLELAAIVLLFG
                     AQVIAEYERIELEPVRRPPSALRT"
     gene            complement(2251..>4201)
                     /locus_tag="VFO53_10210"
     CDS             complement(2251..>4201)
                     /locus_tag="VFO53_10210"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HET8876689.1"
                     /translation="PDASTAAAPDADAAAGHAPSTPAFTPFSHPRRRATDRLEGDGAR
                     AMLRVRADVVDQLVNEAGEVAIARARVEGELRALKANLLELTGSVIRLRSQVREIELQ
                     AETQIQSRMSAASPSQEDFDPLELDRFTRFQELTRSLAEGVNDVSTVQQSLLKNLDDA
                     DAALLAQARMSRDVQQRLFAIRTVPFASLTERLYRILRNTARELDKRANLEIHGGQTE
                     LDRSVLEKLVGPLEHLLRNALDHGLESRAARLAAGKSETGEITLTVRQVGNEIAIDLA
                     DDGAGIDFDRVRERAVALGILAADSEPTIHQLVECLFHPGFSTASEVTQISGRGIGMD
                     VVRNEIAALGGRVDVHTAPGKGTRFNLFLPLTLAVAQAVLVRAGGRMWALPATMVDQV
                     QQVKADVLRSLYADARVHWQGRAWPFHYLPRLLGDVASVPEMARYNAVLLVRSGQGTA
                     AIHVDEMLGNQEIVVKNIGSQLARVPGFSGATVLGTGEIVLIINPVQLAQRGGVLRYD
                     PADDARYAAEQHAASLAVASRRLVMVVDDSLTVRKFTTRLLTREGFEVVTARDGVDAL
                     KLLSEHTPDVILLDIEMPRMDGFEFAKTIKGDAKTAAIPVIMITSRTADKHRNRAAEL
                     GVDRFLGKPYQEDELLATLREVVQA"
BASE COUNT          631 a         1438 c         1390 g          742 t
ORIGIN      
        1 cgatgtattt cgccccctgc agcatgaaca gatgcccgac cttgaacggc ttgcggccat
       61 cgccgacgaa gagctgcgtg tcgcgcgtca ccgtgccctg gtgcacgcgg aacacgccga
      121 gcttgccgac gaacgggtcc atcacgacct tgaagacatg cgcaagcacg tgcttcgacg
      181 gatcgggctc ggagtggaat tcgaccgcgt tatcgccttc gcccttgatg aaaagcggcg
      241 gattgccttc ggccggattc ggggcgagcc tcaccaggac gtcgagcagc tcggagacgc
      301 cggcgccggt gcgcgcggac acgaagcaca ccggcaccag atgtccttcg cgcaacgcct
      361 tctcgaacgg cgcatgcagc tgttcgggcg caacctcccc ctgctcgaga tagagcgaca
      421 tcagctcctc gtcgacttcg acgacctggt cgacgagcgc gcggtgcgcc tcggcgaccg
      481 atgaaaaatc ggactcgccc gccggattga agaagcagtc gacgacgtgg ctcgcgccac
      541 ccgcgggcag gttgatcggc aggcattccc tgccgaacgc ctcgcgcacc gcctcgagca
      601 cgccgggaag gtcgacgttc tcggcgtcga tccggttgat cacgacgagc cggcagagct
      661 tgcgttgcgc cgcccattcc atcatccgtg cgccgatcat ctgcacgctc gtcgccgcat
      721 tcaccacgac cgcggcggtt tccaccgcat cgagcgcacc gatcgactga ccgatgaaat
      781 cgggcagccc gggcgtgtcg atcatgtgga tgcgcgtgcc ggcggtctcg aggtgcacga
      841 gcgacgcgcg cagcgaatgc tgatacgtct tttcgagcgg gtcgaaatcg ctgaccgtgc
      901 tgccgcgctc gacgctgccc gcggcggcga tcgcgccggc cttcgtcagc agcgcttcga
      961 cgagactcgt cttgcccgag cctccgtcgc ccacccacgc tacggtacga acgttttccg
     1021 tcgcgaattc tgccatggcc gtctcctcgt gtggaatgga ggaaatgtac cgccgatgtc
     1081 cggcgctgtc agcctcgcgg cacccggcgc ccggcccgcg ttccgcgttt gtcctacgcg
     1141 ctcgcgaggc ggcgccccga tggccgcgcg acagtcgcca tgcgagaatc gacgacgtgc
     1201 tcgcaacgct tttgcctcgg tcggggcttt cacgcgcggg gcgcaggatt gcgcacgacc
     1261 cgctcggctt cgcgctgcag acgctgcgcg cgttccgcgc gaaccagggc ctgctgctcg
     1321 ccggcggcgt ggcctactac acgctgctgt cgatcgtgcc gctgatgatc ctgctcgtga
     1381 tcatcctgtc gcacgtggtc gatgcggcgc ggctgatgtc gacgctcgcg cgctatctcg
     1441 agatcgtcgc gcccggacaa tcgggcccgc tgatcgacga gttgcgctcg ttcgtcgcgc
     1501 accgcgaagt gatcggctgg acactggcga tcacgctgat cttcttcagc tcgctcgcat
     1561 tctcggtgct cgagaacgcg atgtccgtca ttttctatca ccgtgtgctc acgaagcggc
     1621 gaccgttcgt gatctcgatt ctgatcccgt acctctatat cctgtttctc ggcatcggcc
     1681 tcctggtcgt cacgctggtc gcgggcgcgc tgcagtcgct cgcgctgacc acgctcgacg
     1741 tgctcggcca cgactggtcg ctgtcgggcg tctcgcgcac gcttctctac gtgatcggcg
     1801 tcttcggcga agtgctggtg ctcgcgtcga tctatttcgt gatgccggtc gggcggctgt
     1861 catggcgcca cgcgttgatc ggcggcgtca gcgccaccgt gctgtgggag atctcgcgcc
     1921 acgtgctcgt ctggtacctt gcgacgctgt cgcaggtgca gacggtctat ggctcgctca
     1981 cgacgtcgat cgtcgtgctg ctgtcgctcg agctcgccgc gatcgtgctg ctgttcggcg
     2041 cgcaggtcat cgccgagtac gagcggatcg aactggagcc ggtgaggcgg ccgccgagcg
     2101 cgttgcgcac ctgaacaggg tcagagacga ctttcgacga cgcgcgtcga cgagggcaac
     2161 ggatgctcgc cgcgcatttg atcggggtca gagctgtaat tattcaccgg cgccgaacaa
     2221 ttacagctct gaccccgatt acgtagcctc ctacgcctgc accacctcgc gcaaggtcgc
     2281 cagcagctcg tcttcctggt acggcttgcc gagaaagcga tcgacgccga gctcggccgc
     2341 gcgattgcga tgcttgtcgg cggtgcgcga cgtgatcatg atcaccggga tcgccgcggt
     2401 cttcgcgtcg cccttgatcg tcttcgcgaa ttcgaagccg tccatccgcg gcatctcgat
     2461 atcgagcagg atcacgtcgg gcgtgtgctc ggacaggagc ttcagcgcgt cgacgccgtc
     2521 gcgggccgtc acgacctcga agccttcgcg cgtgaggaga cgcgtcgtga acttgcgcac
     2581 ggtaagcgaa tcgtcgacca ccatcacgag acgccgcgat gcaaccgcaa gcgacgccgc
     2641 gtgctgctcc gccgcgtaac gggcgtcgtc ggcggggtcg tagcgcagca cgccgccgcg
     2701 ctgcgcgagc tgcaccggat tgatgatcag cacgatctcg ccggtgccga gcaccgttgc
     2761 accggaaaag ccgggtacgc gcgcaagttg cgagccgatg ttcttgacca cgatttcctg
     2821 gttgcccagc atctcgtcga cgtggatggc ggccgtgccc tggccgctac gcacgagcag
     2881 caccgcgttg tagcgcgcca tctccggcac gctcgccaca tcgccgagga gccgcggcag
     2941 gtaatggaac ggccacgccc ggccctgcca gtgcacgcgc gcgtcggcgt agagcgagcg
     3001 cagcacgtcg gccttgacct gctgcacctg gtccaccatc gtggccggca acgcccacat
     3061 ccgcccgccg gcgcgcacga gcaccgcctg cgcaacggca agcgtcagcg gcaggaacag
     3121 gttgaagcgc gtgcccttgc ccggcgccgt atgcacgtcg acgcgcccgc ccagggcggc
     3181 gatttcgttt cgcaccacat ccatgccgat gccgcgaccc gagatctgcg tcacctcgga
     3241 cgccgtggaa aagccgggat ggaacaggca ctcgaccagc tggtggatcg tcggctcgct
     3301 gtccgccgcg aggatgccga gcgcgaccgc gcgctcgcgc acgcggtcga aatcgatgcc
     3361 ggcgccatcg tcggcgaggt cgatcgcgat ttcgttgccg acctggcgca ccgtgagcgt
     3421 gatctcgccc gtttcggact tgcccgccgc aagtcgcgcc gcgcgcgatt cgagcccgtg
     3481 gtcgagcgcg ttgcgcagca ggtgctcgag cgggccgacg agcttttcga ggaccgagcg
     3541 atcgagctcg gtctggccgc cgtggatttc gagattggcg cgcttgtcga gctcgcgcgc
     3601 cgtgttgcga agaatccggt agagccgttc ggtgaggctc gcgaacggca cggtgcggat
     3661 tgcgaacagg cgctgctgca cgtcacgcga catgcgcgcc tgcgcgagca gtgcggcgtc
     3721 ggcatcgtcg aggttcttca gcagcgactg ctgcaccgtc gacacgtcgt tgacgccttc
     3781 ggcgagcgaa cgcgtcagct cctggaaacg cgtgaagcga tcgagctcga gcgggtcgaa
     3841 gtcctcctgc gacgggctcg ccgccgacat gcgcgactgg atctgcgtct cggcctgcag
     3901 ctcgatctcg cggacctggc tgcgcaggcg gatcacgctg ccggtgagct cgagcaggtt
     3961 cgccttcagt gcgcgcagtt cgccctcgac gcgcgcccgt gcgatggcga cctcgcctgc
     4021 ctcgttcacg agctggtcga cgacatcggc gcgcacgcgc agcatcgcgc gggcaccgtc
     4081 gccttcgaga cggtcggttg cgcgccggcg cggatgcgag aacggcgtga acgccggcgt
     4141 cgatggcgcg tggcccgccg ccgcatcggc gtccggtgcg gccgcggtcg atgcgtcggg
     4201 t
//
LOCUS       DASSVX010000235         2705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_476832, whole genome shotgun sequence.
ACCESSION   DASSVX010000235 DASSVX010000000
VERSION     DASSVX010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476832"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="VFO53_10215"
     CDS             complement(<1..151)
                     /locus_tag="VFO53_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET8876690.1"
                     /translation="MPEIIVLPHAEICPEGKTFEASSGVSLLDNLLEQGIDVEHACEK
                     SCACTT"
     gene            complement(155..1393)
                     /locus_tag="VFO53_10220"
     CDS             complement(155..1393)
                     /locus_tag="VFO53_10220"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET8876691.1"
                     /translation="MLLAISGQSVASGPVPVPATTARAMAFSRDANLLWLAGRVLLLG
                     IPALVLFTGLAGRLRSTLAGIVRESRFWTVTGCALAYLAIFSLLFLPLEFALRRVLTG
                     YGLSAQSTTAWLAEQGTGFAIKALIVALVAWIPYWLLARSPRRWWLGSALSLLPIVAL
                     LFVLQPLWIDPMFHTYKPLENPRLRQMIAALAARGGLGDVPILESDGDGTDSCGVACV
                     TGLGPSRRIVISDAAAAHYSEREIASIVGHEMKHYVKDDNVKAFAIVSVLLLAGFWLT
                     HQLGGAMVARWSRRFGIRDLADPASIPLLVLCLTLVYVIADPAFLAYARHVEFEADRF
                     GLEITEDNEAAATTLVKDAQLAPILPNPGWFATVFRENHPSIADRIRFANTYHPWLTG
                     GPLVYGGQFMKNESRHASGD"
     gene            complement(1488..>2705)
                     /locus_tag="VFO53_10225"
     CDS             complement(1488..>2705)
                     /locus_tag="VFO53_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET8876692.1"
                     /translation="RGVFEVLATNGDAALGGDDFDHRVYCWALETAQLPPLSPTDARR
                     LMASAREAKEMLTSRGTAPVVASLADGMRLELTLSADTFTDLTRALVAKTLAPVRRAL
                     GDAKLGPADIKGVVLVGGATRMPQIRRAVAGFFGQVPLTNLDPDAVVALGAAIQANKL
                     AGNRESDDWLLLDVIPLSLGIETMGGLVDKIVPRNSTIPVVRAQEFTTFKDGQTAMAL
                     HVLQGEREKVADCRSLARFELRGIPPMVAGAARIRVSFDVDADGLLSVSAREQTSGVE
                     ATVCVKPSYGLTDAEIARMLQESIAHAADDADARALAEARVEAERIVAATEGALADDA
                     TLLDGAERGAIDRALAEVRRRLEGDDRHALSAAVKVLNGATERFAAKRMDRSVARVLT
                     GRRIDALDGHDAS"
BASE COUNT          476 a          936 c          884 g          409 t
ORIGIN      
        1 acgtcgtgca cgcgcacgac ttctcgcacg cgtgctcgac gtcgatgccc tgctcgagca
       61 ggttgtcgag gagcgacacg ccggaagacg cctcgaacgt cttgccctcg ggacagatct
      121 cggcgtgcgg caggacgatg atctcgggca cctgtcaatc gccgcttgcg tggcgtgact
      181 cgtttttcat gaattggccg ccataaacga gcggcccacc tgtaagccac gggtgatacg
      241 tgttcgcaaa tcggattcgg tcggcgatcg acggatgatt ctcgcggaag acggtcgcaa
      301 accagcccgg attcggcaag atcggagcga gttgcgcgtc ttttaccagc gtcgttgccg
      361 ccgcctcgtt gtcctcggtg atctcgagac cgaaacgatc ggcttcgaac tccacgtggc
      421 gcgcgtacgc caggaacgcg ggatccgcga tcacatagac aagcgtcagg cacagcacga
      481 gcagcggaat cgatgccgga tcggcgagat cgcgaatgcc gaagcgacgc gaccagcgcg
      541 caaccatcgc cccgcccaat tggtgggtaa gccagaaccc ggcaagcagc agcaccgaaa
      601 cgatagcgaa ggccttcacg ttatcgtcct tcacgtagtg cttcatctcg tgccccacga
      661 tggacgcgat ctcgcgctcg ctgtaatgcg cggccgccgc gtcgctgatt acgatgcgcc
      721 ggctgggacc gaggccggtc acgcaagcga cgccgcagct atcggtgccg tcgccgtcgc
      781 tttccagtat cgggacgtcg ccgagcccgc cccgcgccgc caaggcggcg atcatctgcc
      841 gcaggcgcgg gttttcgagc ggcttgtacg tgtggaacat cggatcgatc cacagcggct
      901 gcaacacgaa caacaaggcg acgatcggca gcagcgaaag cgcgctcccc agccaccagc
      961 ggcgcggact gcgggcgaga agccaatagg ggatccacgc gacgagcgcg acgatcagcg
     1021 ccttgatcgc gaatccggtt ccctgttccg ccagccacgc ggtcgtgctt tgcgcggaaa
     1081 gaccgtagcc ggtcaggacg cgacgcagcg cgaattcgag cggcaggaac aacaacgaaa
     1141 aaatcgcgag gtaggcgagc gcgcaacccg taaccgtcca gaaacggctt tcgcgcacga
     1201 tgcccgcgag cgtcgatcgt aagcggccgg ccagccccgt gaacaacacc aatgcgggta
     1261 tgccgagcag caataccctg ccggcgagcc aaagcagatt tgcatcgcgc gagaacgcca
     1321 tcgcgcgcgc cgtcgttgcc ggcaccggta ccggccccga ggcgaccgat tgcccggaaa
     1381 ttgcgagcag catgagcccc aatgcgagga tcgcactccg cgtgcaacgc gccccgcggc
     1441 ggggggaaga aacggtttcg ggcggcgatc gccatcccaa tgcgcggtca cgaagcatca
     1501 tggccgtcga gcgcatcgat gcgcctgccg gtcagcacgc gcgccacgct gcgatccatc
     1561 cgtttcgccg cgaagcgctc ggtggcgccg ttcagcacct tgaccgccgc cgacaatgca
     1621 tggcggtcgt cgccctcgag ccggcgacgc acttcggcga gcgcgcgatc gatcgcgccg
     1681 cgctcggcgc cgtcaagcag cgtcgcgtcg tcggcgagcg cgccttcggt ggccgcgacg
     1741 atgcgttccg cttcgacgcg cgcctcggcg agcgcccgcg cgtcggcgtc gtcggcggcg
     1801 tgggcgatcg attcctgcag catccgcgcg atctctgcat cggtcagccc atacgacggc
     1861 ttgacgcaaa ccgtcgcttc gacgccgctc gtctgctcgc gtgccgacac cgacagcagc
     1921 ccatcggcat cgacgtcgaa cgacacgcga atgcgcgcgg cacccgcgac catcggcggg
     1981 atgccccgca actcgaagcg cgcgagcgag cggcagtcgg cgaccttttc gcgctcgccc
     2041 tgcagcacgt gcagcgccat cgcggtctgt ccgtccttga acgtggtgaa ttcctgcgcg
     2101 cgcaccacgg ggatcgtcga gttgcgcggc acgatcttgt cgaccagccc gcccatcgtc
     2161 tcgatgccaa gcgacagcgg gatcacgtcg agcagcagcc agtcgtcgct ctcgcggttg
     2221 ccggcgagct tgttcgcctg gatcgccgcg ccgagcgcca ccaccgcgtc gggatcgagg
     2281 ttggtgagcg gcacctggcc gaagaagccg gcgaccgcgc gccggatctg cggcatgcgc
     2341 gtcgcgccac cgacgagcac gacgcccttg atgtcggccg ggccgagctt cgcatcgccg
     2401 agcgcgcgcc tgaccggtgc cagcgttttt gcaacgagtg cgcgcgtaag gtcggtgaac
     2461 gtgtcggcgc tcagcgtgag ctcgaggcgc atgccgtcgg cgaggctggc cacgaccggc
     2521 gcggtaccgc ggctcgtcag catttccttc gcttcgcgcg cgctggccat caaccgccgc
     2581 gcgtcggtcg gcgacaacgg cggcagttgc gcagtttcga gtgcccagca gtacacgcgg
     2641 tgatcgaagt cgtcgccacc gagcgccgca tcgccattcg tcgccagcac ctcgaacacg
     2701 ccgcg
//
LOCUS       DASSVX010000236         3530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1376864, whole genome shotgun sequence.
ACCESSION   DASSVX010000236 DASSVX010000000
VERSION     DASSVX010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376864"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /gene="lipA"
                     /locus_tag="VFO53_10230"
     CDS             <1..913
                     /gene="lipA"
                     /locus_tag="VFO53_10230"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET8876693.1"
                     /translation="GAAKTARIPIKVVEAERLRKPDWIRVRAPSSPRFYEIKQILREH
                     RLHTVCEEASCPNIGECFGKGTATFMIMGDLCTRRCPFCDVGHGRPLPLDADEPINLA
                     QTIAALRLRYVVITSVDRDDLRDGGAAHFVDCIRAVREHSPQTQVEVLVPDFRGRLDR
                     ALDILSAAPPDVMNHNLETVPRLYRQARPGSDYAHSLRLLQSFKARFPALPTKSGLMV
                     GLGETDDEILQTMRDMRAHDIDMLTIGQYLQPSAQHLPVLRYVHPDMFARFEREAYAM
                     GFRHAAVGALVRSSYHADKQAEEVLAG"
     gene            1035..2036
                     /locus_tag="VFO53_10235"
     CDS             1035..2036
                     /locus_tag="VFO53_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876694.1"
                     /translation="MRLVGVIAASAMLAVPTIARAEASVVRISYGFGILYLPLMVMDR
                     EHLLEKHAKAAGLPDVKVEWKTFDGGNVINDAMLSGALDIAGIGVPGFLTLWAKAKDN
                     PRVEVRGLTGMSSSSMYLMTRNPAVKTLADFTDKDRIAVPGIKTSLPAVVLHMAAAKE
                     LGDANYAKLDPITVGLPHPDALAVMLSGKGEIDSHMASPPFSYIEAESPALHRVFNSV
                     DILGNITLDMTFSTRRFVEANPKLAAAFVAAMQEACAAIGADKLKAAQIYNDVRKSKQ
                     PVEETLKILEDPNSKFSAVPDGTMRYAEFMSRIGTIKAKPASWKDLFFDTILSAQGS"
     gene            complement(2059..3288)
                     /locus_tag="VFO53_10240"
     CDS             complement(2059..3288)
                     /locus_tag="VFO53_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HET8876695.1"
                     /translation="MPLLPDRHATATLMRVARSALVAVAIALAGCQVAPPVVQPPPGP
                     ARAVFTPATYADLPGWSADRVQDAWPAFLVGCKALVARTRTQPLWQVPCAAAESVDVQ
                     DANAVRRFFEQNFTPYRITGSDGSEQGLVTGYYEPLLDGSRTQSARYAAPLYAPPDDL
                     LIVELADLYPELKGKRVRGRLDGRRVVPYWPRADIDAGRAATNGKALVYVSDPVDAFF
                     LQIQGSGRVALDDGSVIRVGYADQNGQPFRSVARVLIDRGELTLGEASMQGIRAWGRA
                     HPDQLPALLDENPSYVFFREVPPPPQESLEAAIDGPIGSLGVPLAAGRAIAVDPRAIP
                     LGAPLFLATTQPLSDIPLQRLVLAQDTGGAIRGPIRADFFWGFGDEAGQHAGRMRQQG
                     SEWLLWPNGAALPNAPP"
     gene            complement(3428..>3530)
                     /locus_tag="VFO53_10245"
     CDS             complement(3428..>3530)
                     /locus_tag="VFO53_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HET8876696.1"
                     /translation="GTMRGSYHMVAEDGLAFDVPIAPFTLSIPRTLH"
BASE COUNT          585 a         1214 c         1173 g          558 t
ORIGIN      
        1 gggcgctgcc aagaccgcgc gcattccgat caaggtcgtc gaggccgagc gcctgcgcaa
       61 gcccgactgg attcgcgtgc gcgcgccgtc gtcgccgcgc ttctacgaga tcaagcagat
      121 cctgcgcgag caccggctgc acaccgtgtg cgaggaagcg tcgtgcccga acatcggcga
      181 gtgcttcggc aagggcacgg ccacattcat gatcatgggc gacctctgca cgcgccgctg
      241 cccgttctgc gacgtcggcc atggccggcc gctgccgctc gacgccgacg agcccatcaa
      301 cctcgcgcag acgattgccg cgctgcgatt gcgctacgtg gtcatcacga gcgtcgatcg
      361 cgacgatctt cgcgacggcg gtgctgcgca cttcgtcgac tgcatccgcg cggtgcgcga
      421 gcattcgccg cagacgcagg tcgaggtgct ggtgcccgat tttcgcggcc ggctcgaccg
      481 cgcgctcgat atccttagcg cagcgccacc cgacgtgatg aaccacaacc tcgaaacggt
      541 cccgcgcctc taccggcagg cgcgtccggg gtccgactac gcgcattcgc tgcggctcct
      601 gcagtcgttc aaggcgcgct tccccgcgct tccgacgaag tcggggctga tggtgggtct
      661 cggcgaaacc gacgacgaaa tcctgcagac gatgcgcgac atgcgcgccc acgacatcga
      721 catgctgacc atcggccagt acctgcagcc gtcggcacag cacctgccgg tcctgcgcta
      781 cgtccatccc gacatgttcg cccggttcga gcgcgaggcg tatgcgatgg gcttccgtca
      841 cgcggcggtt ggcgcgctgg tccgctcgtc gtaccacgcg gacaagcagg ccgaggaagt
      901 gctagccggc tgaccggcga tcgtacgggc cgtcgccggt ttgcgagcat cgcacgctgg
      961 cgcgcatcgc gtcgcttatc atcgagtttt cattcggagc cgccatgtgc gtaagtgcat
     1021 tccgttcgct tgccatgcgc cttgtcggcg taatcgccgc ctcggcgatg ctcgccgtcc
     1081 ccacgatcgc gcgcgccgaa gcgtcggtcg tccgcatctc gtacggtttc gggatcctct
     1141 acctgccgct gatggtgatg gaccgcgagc atctgctcga aaagcatgcg aaggccgcgg
     1201 gactgccgga tgtgaaggtc gaatggaaaa cattcgacgg cggcaacgtg atcaacgacg
     1261 cgatgctctc gggcgcgctc gacatcgcag gcatcggggt gccaggcttc ctcacgctgt
     1321 gggccaaggc gaaggacaac ccacgggtcg aggtgcgcgg cctcacaggc atgagcagct
     1381 cgtcgatgta cctgatgacg cgcaatcccg ccgtgaagac gctcgccgac ttcaccgaca
     1441 aggaccgcat cgcggtcccc ggcatcaaga cgtcgctgcc ggccgtggtg ctgcacatgg
     1501 cggcggcgaa ggagttgggc gacgccaatt acgcgaagct cgaccccatc accgtcggcc
     1561 tgccgcatcc cgacgcgctc gccgtgatgc tttccggcaa gggcgagatc gattcgcaca
     1621 tggcgtcgcc gcccttctcg tacatcgaag ccgagtcgcc cgcgctgcat cgcgtcttca
     1681 attcggtcga catcctcggc aacatcacgc tcgacatgac cttctcgacc cgccgctttg
     1741 tcgaggccaa tccgaagctc gctgcggcgt tcgtcgcggc gatgcaggag gcctgcgccg
     1801 cgatcggcgc cgacaagctc aaggcagcgc agatctacaa cgacgtgcgc aagagcaagc
     1861 agccggtcga ggaaaccttg aagatcctcg aggatccgaa ctcgaaattc agcgccgttc
     1921 cggacggcac gatgcgctac gcggaattca tgtcgcgcat cggcacgatc aaggcgaagc
     1981 ccgcgtcgtg gaaagatctg ttcttcgaca ccatcctgtc cgcacaggga agctgacgca
     2041 gcgtctggct cgcgggtgtc acggcggcgc gttcggcaac gccgcaccat tgggccagag
     2101 cagccactcg ctgccctgct ggcgcatccg tcccgcatgc tgcccggctt cgtcgccgaa
     2161 gccccagaaa aagtcggcgc gaatgggacc acgtatcgcg ccgcccgtgt cctgcgccag
     2221 gacgaggcgt tgcagcggga tatccgacag cggctgtgtc gtagcgagga acagcggcgc
     2281 gccgagcggg atcgcgcgcg gatcgaccgc gattgcacgc ccggcggcga gcggcacgcc
     2341 gaggctgccg atcggcccgt cgattgccgc ctcgagcgac tcctgcggtg gcggcggcac
     2401 ttcacgaaag aacacgtagc tcggattttc gtcgagcagc gccggcaact ggtcggggtg
     2461 cgcgcgaccc catgcgcgaa tgccctgcat cgacgcttcg ccgagcgtca gctccccgcg
     2521 atcgataagc acgcgcgcca ccgagcgaaa cggctgtccg ttctggtcgg cgtagccgac
     2581 gcgaatcacg ctgccgtcgt cgagcgcgac ccgccccgag ccctggatct gcaggaagaa
     2641 tgcatcgacc gggtcgctca cgtacacgag cgccttgccg ttcgtggcgg ccctgccggc
     2701 gtcgatgtcg gcgcgcggcc agtacggcac cacacgccgg ccatcgaggc gtccgcgcac
     2761 gcgcttgccc ttgagctcgg gatagagatc ggcgagctcg acgatcagga ggtcgtcggg
     2821 cggcgcgtac agcggtgctg cgtagcgcgc gctctgcgtt cgcgagccgt cgagcagcgg
     2881 ctcgtaatag ccggtgacga ggccctgctc gctgccgtcg ctgccggtga tccgatacgg
     2941 cgtaaaattc tgctcgaaga atcgccgcac ggcattcgcg tcctgcacat cgaccgactc
     3001 ggccgccgcg cagggcacct gccacagcgg ctgcgtgcgc gtgcgcgcca ccagcgcctt
     3061 gcagcccacg agaaacgccg gccacgcgtc ctgcacgcga tcggcgctcc agccgggcag
     3121 gtccgcatac gttgcgggcg tgaacacggc gcgcgccggt cccggcggtg gctgcacgac
     3181 cggcggcgcc acctggcatc cggcgagcgc gatcgcaacc gcgacgagcg cgcttcgcgc
     3241 gacgcgcatc agcgtggcgg tggcatgtcg atcggggaga agtggcaatg gcctggctcg
     3301 tattcgaatc gttcgtcgca ttgctgattc tggtcgggat cgtcgcgtgg acgatggcgc
     3361 cgagccgcaa gcgcgaacgg cggccggacg acgaggatgg agccgcgccg gcgcggcgca
     3421 cgcggagtca atgcagcgtg cgcggtatcg acagcgtgaa cggcgcaatg gggacgtcga
     3481 acgcgaggcc gtcctccgct accatgtgat acgacccgcg catcgtgccg
//
LOCUS       DASSVX010000237         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_27029, whole genome shotgun sequence.
ACCESSION   DASSVX010000237 DASSVX010000000
VERSION     DASSVX010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27029"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..948
                     /locus_tag="VFO53_10250"
     CDS             58..948
                     /locus_tag="VFO53_10250"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET8876697.1"
                     /translation="MELYQLRSFAAVAELGHLTRAAERLHVSQPAVSAQIKALEDDLS
                     VLLFERGAAGMTLTAAGRELLPEALRVVAAAQSLRSHALALRGELGGRVQLGSVADPE
                     LTRIADVLRYAVGRFPLLEIDVHQEISGIAFGKVRDGELDTSFYFGNAVHPAVASMPL
                     RKLAFCIVIPAAWAKRLAGASWDTLAAEPWIMTPPVSTHYALATDLFAAHGIAPSRRV
                     EADHEAVVNSLVAAGLGVALMREDLAEPARGTIAIWGDTRIVTSLAFLYRREREDDPP
                     IRALRGVVRAVWKVPADTTK"
     gene            1018..1581
                     /gene="msrB"
                     /locus_tag="VFO53_10255"
     CDS             1018..1581
                     /gene="msrB"
                     /locus_tag="VFO53_10255"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006171950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET8876698.1"
                     /translation="MNRRLLLQAFAAALLMRAGLTPAQFVTKSAPKAASVDDLQRHWK
                     LLLASDAKVATSATPIVKSDDEWRKSLDPAAYDVLRHEGTERPFTSPLNDEHRPGVFV
                     CAGCALPLFTSAMKFDSGTGWPSYFTTIPGVFATTTDHKLIVARTEYHCVKCGGHHGH
                     IFDDGPAPTGLRYCNNGVALRFIPAQV"
     gene            1620..2303
                     /locus_tag="VFO53_10260"
     CDS             1620..2303
                     /locus_tag="VFO53_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876699.1"
                     /translation="MVELRDVAKAFAGPSPREIFRNLSLTLGRGEYVAVVGASGVGKS
                     TLLNIIAGLEPVDAGRVIVDGVDCALLDDEARTTFRRDRMGFVFQAFHVLPYLSVAAN
                     VALPLALQGSAGASADARVSAVLRAVGLDGRNASSPRELSGGELQRVAIARALVHRPS
                     LVLADEPTGNLDPQTARQVIALLRDTVKQCGAAAVLATHSRAVAASADRVYRLEKDGL
                     HAASAADIE"
BASE COUNT          352 a          806 c          782 g          382 t
ORIGIN      
        1 acttgatcgg ccattcgatc cggtattgca cggcgtcatg aacgccgtcg cgtgaccatg
       61 gagctctacc agctgcgcag cttcgcagcg gtggctgagc tcggccacct gacccgcgcc
      121 gccgaacggc tgcatgtcag ccagccggcg gtgtcggcgc agatcaaggc gctcgaggat
      181 gacctgtcgg tgctgttgtt cgagcgcggt gccgcgggca tgacgctcac ggcggcgggt
      241 cgggagctgc tgccggaagc gctgcgtgtc gtcgcggccg cgcagagcct gcgcagccac
      301 gcgctggcac tgcgcggtga gctcgggggc cgcgtgcagc tcggcagcgt cgcggatccc
      361 gagctaacgc gaatcgccga cgtcctccgg tatgcggtcg gccgctttcc gttgctcgag
      421 atcgatgtcc accaggagat ttcgggcatc gcattcggca aggtccgcga cggcgagctc
      481 gacacgtcgt tctatttcgg caacgcggtc cacccggccg tcgcatcgat gccattgcga
      541 aaactcgcgt tctgcatcgt gattcccgcg gcatgggcga agcggctcgc cggcgcctcg
      601 tgggatacgc tggccgcgga gccgtggatc atgacgccgc cggtgagcac ccactacgcg
      661 ctcgccaccg acctcttcgc cgcgcatggc atcgcaccgt cgcggcgcgt ggaagccgat
      721 cacgaggcgg tcgtgaattc gctcgtcgcg gccgggctcg gcgtcgcgct gatgcgcgag
      781 gatcttgcgg aaccagcgcg cgggacgatc gcgatctggg gcgacacgcg tatcgtcacg
      841 tcgctcgcct tcctgtatcg ccgcgagcgc gaggacgatc ctccgatccg ggcgctgcgc
      901 ggcgtcgtgc gcgctgtatg gaaagtgcct gccgatacga ccaagtgacg tgcaatgccc
      961 ggaacgatcg tagcagcgcg ggcgtccaac ccaccgaccg acaagacgga gtgctcaatg
     1021 aatcgtcgtc ttctgctgca agcctttgcc gccgcgctcc tgatgcgcgc cggccttact
     1081 cccgcacaat tcgtgacgaa gtccgccccg aaggccgcga gcgtcgacga tctgcaacgc
     1141 cactggaagc ttttgcttgc aagcgatgcc aaggtcgcga cgagcgccac gccgatcgtc
     1201 aagtcggacg acgaatggcg caagagcctc gatcctgccg cttacgacgt gctgcgccac
     1261 gaaggcaccg agcgcccgtt cacgagcccg ttgaacgacg agcaccggcc gggcgtgttc
     1321 gtgtgcgccg gctgcgcgct gccgctcttc acgtccgcga tgaagttcga tagcggcacg
     1381 gggtggccaa gctatttcac gaccatcccc ggcgtcttcg ccacgacgac cgaccacaag
     1441 ctgatcgtgg cacgcaccga atatcactgc gtgaaatgcg gcgggcacca cggacacatc
     1501 ttcgacgacg gacccgcgcc caccgggctt cgctactgca acaacggcgt cgcgttgcgc
     1561 ttcattcccg cgcaggtgtg acgcaagcgt gggtcgatgg ccgcgccatc gacccaccca
     1621 tggtcgagct gcgcgatgtc gccaaggcgt ttgccggtcc ctcgccgcgc gaaatcttcc
     1681 gcaacctgtc gctgaccctc gggcgcggcg agtacgtcgc cgtcgtcggc gcgtcgggtg
     1741 tcggcaagtc gacgttgctc aacatcattg ccggcctcga gccggtcgac gcgggtcgcg
     1801 tgatcgtcga cggcgtcgat tgcgccctgc tcgacgacga ggcgcgcacg acctttcgcc
     1861 gtgacaggat gggcttcgtg ttccaggcgt ttcacgtgct gccgtacctg tccgtggccg
     1921 cgaacgtcgc gctgccgctc gcgctgcaag ggtcggcggg cgcatccgcc gatgcacgcg
     1981 taagcgcggt gctgcgcgcg gttggactcg acggccgcaa tgcgagctcg ccgcgcgagc
     2041 tttccggcgg cgagttgcag cgcgtggcga tcgcgcgcgc gctcgtgcac cggccgtcgc
     2101 tcgtgctcgc cgacgagccc accggcaacc tcgacccgca gaccgcgcga caggtgatcg
     2161 cgctgctgcg cgacacggtg aaacaatgcg gcgccgcggc cgtgctcgcg acgcattcgc
     2221 gcgcggtcgc ggcgtcggcc gatcgcgtgt accggctcga gaaggacggc ctgcacgccg
     2281 catccgccgc ggacatcgaa tgaatcccgt cgcgcagcgt gc
//
LOCUS       DASSVX010000238        23564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1526626, whole genome shotgun sequence.
ACCESSION   DASSVX010000238 DASSVX010000000
VERSION     DASSVX010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 23564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23564
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526626"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..987)
                     /locus_tag="VFO53_10265"
     CDS             complement(226..987)
                     /locus_tag="VFO53_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876700.1"
                     /translation="MNLHAVRAIYRFEMARTRRTLLQSIVSPVISTSLYFVVFGAAIG
                     SRIQQVGGVSYGSFIVPGLIMLSLLTQSISNASFGIYFPKFTGTIYELLSAPVSYFEI
                     VMSYVGAAASKSIILGLIILGTAALFVPLHIEHPVWMVLFLVLTAVTFSLLGFIIGIW
                     ADGFEKLQLVPLLIVTPLTFLGGSFYSIDMLPPFWQKATLANPVVYLVSGFRWSFYGI
                     ADVDIAVSLAMTIAFLVVFLVIVAWIFRTGYRLKS"
     gene            complement(984..1913)
                     /locus_tag="VFO53_10270"
     CDS             complement(984..1913)
                     /locus_tag="VFO53_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876701.1"
                     /translation="MPPIISISNLSKTYASGFAALKRVDLDIRRGEIFALLGPNGAGK
                     TTLISIVCGIVNATEGRVLVDGKDIGRDYRETRALIGLVPQELTTDAFETPWGALSFS
                     RGLFGKPPDPAHIEKVLRDLSLWNRKDSRIMTLSGGMKRRLLIAKALAHEPRILFLDE
                     PTAGVDVELRRDMWSLVRSLRSGGVTVILTTHYIEEAEEMADRIGVISKGELILVDDK
                     VELMRKLGRKQLTLHLQDPLERIPPALAGYKLELVNGGNDLTYTYEADSVRSGIASLL
                     EQLDLAGIRFKDLQTSESSLEDIFVSLVHEQRP"
     gene            complement(1985..2692)
                     /locus_tag="VFO53_10275"
     CDS             complement(1985..2692)
                     /locus_tag="VFO53_10275"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HET8876702.1"
                     /translation="MQADLVDANVDFHGDLVRNIPGIRQSQHLFDDLSADPADWDVAI
                     AAEAAARIATDAPVVTRPFDYGSVITYTFDASHWQATRFSDGTEYGVWYGSMSVETTV
                     CETAWHWYRFVLDSYAGEDRDVVTERRLFDVRCDALLIDLRGKQAKHPALTSRTSYVF
                     TQRIGRFLHQQDQNGLLVQSARCDGINACVFKPQRLSNVRHRAYLTYRLNAVRDTFVA
                     ERTRGRKWLALVPSALG"
     gene            complement(2720..3124)
                     /locus_tag="VFO53_10280"
     CDS             complement(2720..3124)
                     /locus_tag="VFO53_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HET8876703.1"
                     /translation="MRVVARKRVAIEPGSREARGRLAAMVTTLLEHWKLTTAEQADVL
                     GLSTGSRSTLGRYRSGEPLADNRDLLDRAGHLLGIHKSLRILFPHDRDLAYRWMTQPN
                     RRLGTRPVDMVLEHGFEGLLALRRYLDFQRGS"
     gene            complement(3238..4008)
                     /locus_tag="VFO53_10285"
     CDS             complement(3238..4008)
                     /locus_tag="VFO53_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019567166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET8876704.1"
                     /translation="MNLPINRFKRALAAGQSQIGLWCSLSSHYSAEVVAGAGFDWLLL
                     DTEHSPNDLESLLMQLQAVAAYPSTPVVRVPWNDTVTIKRVLDIGAQSLLIPYVQDAV
                     EARAAAKAMRYPPAGVRGVAGATRATRFGRVSDYAQHAHEELCLLVQLETEQALQHID
                     AIAQIDEIDGIFIGPADLHASMGYAGEVANPKVVPLIEDALRRIRRSGKAPGILVGDA
                     QLAKRCIEAGSLFTAVGVDVAILARGAERLAAQFGRAS"
     gene            complement(4055..4498)
                     /locus_tag="VFO53_10290"
     CDS             complement(4055..4498)
                     /locus_tag="VFO53_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET8876705.1"
                     /translation="MSNPGYALALEAADTIVDAVLASARAAKLRPLAVVVLDAGGHDI
                     VLKREDGCGILRVDIARGKAWGALGMGLGSRELARKAEQNPRFFAALAAASGGRLIPN
                     PGGVLVRDIGGEVVGAVGVSGDQSDHDEACAIAGIEAAGLRADPG"
     gene            complement(4495..5853)
                     /locus_tag="VFO53_10295"
     CDS             complement(4495..5853)
                     /locus_tag="VFO53_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET8876706.1"
                     /translation="MNAEKLLSLVDRKLVVDLATELVNTASATGEEGNMARALERALK
                     EVGCATQLQNIYDDRYNCIGRLAGSGAGPTILMSGHMDTSVRGDEDYLQGKGWKNHAV
                     LDDDRIWGNGICNMKNAFVSYIAGIDAMRRAGVRLGGELVIAGTAGEIEMAAIDEFQG
                     KHYHGYGMGLRYMLIHGVTADFHFLGEPTAQVPSTGVMGTTWAKVSVHGDFAHSAFHD
                     STLSALDEMWLLWQELGAWIAEYKKQHTYQGIVPAVNRACMRGGLPWRAARTCNLAQL
                     YVDIRFPPNRYPIDVQREFTEVVTRIAQQKLQRPVDIEYYMARPGTEISATHAVVQSV
                     VDAHHETTGADVPARFCPPYCTDAIDANRLGIPTCIYGSGGTSPTAGPRAGDLRAKEG
                     EFVLIDDMVNEAAVMMRAAMRLNDIPRDRVVAMRAAMPGVQAAARSAPTDEKIGEPTL
                     QA"
     gene            complement(5948..6799)
                     /locus_tag="VFO53_10300"
     CDS             complement(5948..6799)
                     /locus_tag="VFO53_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876707.1"
                     /translation="MRRAETLPADRAATVARVRIPGVRRIALPAAGRKLLMGTISIGF
                     ALLLWHLSVGTLFNPALVPTPEQTLGKAWRMMLTGELFMHIAVSMRRVLVGYVVGCVV
                     GIAVGALIGRVRIVRELADPMLELIRPISPVAIVPLAMLWFGIGELSKFFVIIYATVI
                     IVLLNTAAGVSRTPVTRIRAARCLGASEYEVFIKIILPSAVPYVLTGMRVALGFSFMG
                     IVAAELIGASEGLGFLIMNSQMLLQTDQLFVGLLSLGIVGLIVDRIFRAVLARSMRRY
                     MQFLGDV"
     gene            complement(6796..7665)
                     /locus_tag="VFO53_10305"
     CDS             complement(6796..7665)
                     /locus_tag="VFO53_10305"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018783782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876708.1"
                     /translation="MNSGATHDRAGKSRTREWSALQFRHVGKVFADSRGGQYAATRDI
                     DVSVRRGDFYCLLGPSGCGKSTLLGMVAGFDPPTSGEIVFVGEDAGVEWTSPIFAAGV
                     DRSMIFQDAAEALFPWLDVEENVLFGPRLRAARARGSAGSAARYLHMVGLQDHGHKFP
                     FELSGGMKQRVQIARALIMEPDILLMDEPFAALDAITKRLLQTEMSRIWQETGKTVLY
                     VTHDIVEALILGTRIAVMTSGPEATIRHEITVDLPRPRRTTDAQFVELVKILEGMLLE
                     EVHKGRRAQGALA"
     gene            complement(7678..8640)
                     /locus_tag="VFO53_10310"
     CDS             complement(7678..8640)
                     /locus_tag="VFO53_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876709.1"
                     /translation="MADHSRRNVLKTLGTAAALAACGIPRLATAQAKLQPMDIYIGTT
                     PHFGNIIVGADKGFFEKEGLPVKITNFASGSVAADAFRTGKGSILVAGDLPSIRLWQQ
                     GFIGLCPQAHYNSLSVIVAQGHVRNASDLKGRKVGVLLGSTSEYFTKLYLSKAGMNVG
                     DIQMINLRPAEMVTGLARGDIDAFVIWQPFGWRATAAVKDAHILATGKGYFEEWEAVS
                     TSKQYAAAHGAELLAFLRGLDAAGRWIPANLDEAAKIVAAQIRFDDVKITRDMIERID
                     WNIAYTPNFRADMDGVGNFIGARIDWQTMFDSRHLQKLGASYVA"
     gene            complement(8851..10263)
                     /locus_tag="VFO53_10315"
     CDS             complement(8851..10263)
                     /locus_tag="VFO53_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8876710.1"
                     /translation="MSSSSAAFIDALRRCIGPANVLVDAAGVAPYVSDWRGAFRGEAL
                     AIACPASTDEVADVVRLCRRERVPVVPQGGNTGMCGGAIPDASRTAIVVALRRMNRVV
                     AVDARNDAITVEAGCLLAQVQEAAAAAGRLFPLSLAAEGSCQIGGNLSTNAGGINVLR
                     YGNARDLVLGLEAVLPDGSVWNGLRALRKDNRGYDLRQLFIGAEGTLGIITGAVLKLF
                     PRPAQRVSALVALREPEDAVTLLASIRERQGDALIAYELIGRACLDLVCAHIHGTRDP
                     FAEPHPWYVLLDVAGSYGGDTLREDLERLLADSAEQGSVADAVIAQTVAQGQTLWRLR
                     ESIPDAIRGAGPALRSDIAVAVDRIPRFIADATDAVLGTMPGVRIVCFGHVGDGNLHF
                     NVLPPAAPEAQSDWSKPLYPVLYDVVDALGGSFSAEHGVGQAKRAELARYKSGVELAM
                     MRTLKQAFDPLNLMNPGKIL"
     gene            complement(10250..11164)
                     /locus_tag="VFO53_10320"
     CDS             complement(10250..11164)
                     /locus_tag="VFO53_10320"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8876711.1"
                     /translation="MTATLDEIGPIAFIGLGHMGAPMAARLASRGASLRVHDLRGDLV
                     ERFVGAHGGIGATSIAAAVDQAAFVIAMLPDDGAVRAVMLGDGGVATSLRRNDAIAID
                     MGTSSPESTLAIAAEFARVGLAYVDAPVMGGVPFASDGTLDVMAGGAPDAVERCTPLF
                     AALGRRTWLCGASGSGHALKAIANFVNAATFIALLEAMTIGKKFGLESAFMAQALAAM
                     CAGRQHPLEKKIVPQVMTRRFGTGMAMGLIAKDVRIVTNLARAIGARAPVAEATHELW
                     REAAQRYGHGRDQAEVVRLWEDGAGVEL"
     gene            complement(11161..11994)
                     /locus_tag="VFO53_10325"
     CDS             complement(11161..11994)
                     /locus_tag="VFO53_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876712.1"
                     /translation="MSRQPVVAIAAMPPLPARRLRVVPFARGVIAIALLLAAWEYFAR
                     SGIFSQAITPPLETIVAALVAMIADGSIFRHTLATMLRVAIGLAIALAIAIPLGMLMG
                     RHPLWERILRPPLAVLMPIPSLAWVPLVVLWFGIGNVATVLIVVYASTFSLLYNVWMG
                     VRTINPLWLRAAQAMNARGVRLFRHVVWPAALPSIITGVRLAFGRAWIAVIGGELLAS
                     PQWGLGKLIFDAKEFLNADVMLAALLVIGVLGLFFERVVFQLLEQVTVNRWGMATGAG
                     R"
     gene            complement(12000..12788)
                     /locus_tag="VFO53_10330"
     CDS             complement(12000..12788)
                     /locus_tag="VFO53_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876713.1"
                     /translation="MNSSRAMSRLGAVAAGSLAFAVMALAWQAAVWAGVFPPKLFPSL
                     ATVAETLLRLTENGTMLAAAAATLFRLLFGFLLAAVAGIVVGLLMGRVQWIEDALLPI
                     VSFMYPIPGIAYAPLFVLWFGLGDVPAILLVAVATSFTVIINTWKGVKAVKPIWIRSA
                     QAMRASERAIFARVILPGALPYVLTGLRLGLASAWRILVAVEMLMSVARGLGWLIFGS
                     QQFLNTDVMLATIAVIGIVGMLLERQLFMRLEKATVVRWGMMSA"
     gene            complement(12785..13768)
                     /locus_tag="VFO53_10335"
     CDS             complement(12785..13768)
                     /locus_tag="VFO53_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876714.1"
                     /translation="MIRSITRRTFLTSTGAAAVALALPHDARADSPPLNFGFQNTSWG
                     SVAMVAEAEKTFEKAGAKVKPFRFDGGKSTRDAMIAGRVDIGVLGAAPFVVGAAKGDI
                     VAIAVAMYAGRTNAVVAAKDSGIRSVAELKGRRVASQLGSSTDYVFQNKILPKFGLAK
                     GDVQIANIPHQNEIAAMVGKSVDAFAGVEPFPSVAEIEGLGTVLVDYGQFDLQPVFVA
                     INRPVLESRRGDVVAFLRGWLAAVRIVNEHPKRAVEIVWNEFKAQGYDVKEAAFTRML
                     AKLDVKPEFTPALRDYLNEQGRVLLGQKQIPAVPDWNRALDESVLAQAKRA"
     gene            complement(13804..14793)
                     /locus_tag="VFO53_10340"
     CDS             complement(13804..14793)
                     /locus_tag="VFO53_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876715.1"
                     /translation="MSKGRIERRDFLKAAGAAALAGAWPLRAQAQAGQPLNLGFQNTS
                     WGTVAMVADAEKTFDKAGASVKFHRFGSGKETRDAMISGRIDVGVLGGTPFVVGAAKG
                     DMGAIGVAMYAGKTNAIVAAKDSGIRTIADLKGKRVASQIGSATNYVFLNKILPKSGL
                     TKDDVHIVNVTFQNQVAAIVGKSADAFAGVEPFPSVAEVEGLGHAIVDYSQFDMQPVF
                     LAVNAPILDKRKNELVAFMRGWLAAVDIVNKEPQRAVRIVFDQFKAQGYDTKEAVFKL
                     MLTKLEVKPQYVPNLKQYLDEQADVLVKEKQISSVPDWNRILDTSVLTQATKA"
     gene            complement(14854..15606)
                     /locus_tag="VFO53_10345"
     CDS             complement(14854..15606)
                     /locus_tag="VFO53_10345"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876716.1"
                     /translation="MAQRIDLMHVSLRYRTEAREVLAVDDVSFTVAESEFLCLLGPSG
                     CGKTTIMNMIAGFLRPTGGEVLIGGARLDAVEHDRGVVFQDFAQLFPWRSARRNVEFG
                     LEMRNMPAAERKRVALEFLRLVGLQEFADAYPHQLSGGMQQRVAIARAIAYSPGVLLM
                     DEPFAALDAMTRDEMQRLVTDVWRETRKTVVYVTHNVAEAVYLGDRVVVLDAHPGRVK
                     AEISLDLPRPRDPLSPPFVDAQRRILAQLGMH"
     gene            complement(15629..16750)
                     /locus_tag="VFO53_10350"
     CDS             complement(15629..16750)
                     /locus_tag="VFO53_10350"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8876717.1"
                     /translation="MISPKPRPVRAALLGTGAWANVLAQAARGSRLEIVRCFSPTGER
                     REAFAAATGIRAVDALDRVWDDADIEAVVIAAPNDRHRALAARAAACGKHVFVEKPIA
                     HTLEDGLAMVALERQHGIVLAVGHCARMLAGNRWIARAIAAGELGRVTHLQATFSNDR
                     GLRLTRGDWRFYSASAPGGPLSQIGIHQFDTLRALGGDLAAVSAQSARLSPVGAEVED
                     QWVVSIRFADGKLGSIVTSWTSPGEYRVQATGDRASVDYAVDQSRWAEPSRLHERAVL
                     ARQARGDGPQARTRIPVPPGNMFRDELEQFADAVRGDGTCELSAANGCQALAAVEAAI
                     VSASRDGAAVTLDEVLAAAGMRGTQAGDAARHANAMAGG"
     gene            complement(16791..17522)
                     /locus_tag="VFO53_10355"
     CDS             complement(16791..17522)
                     /locus_tag="VFO53_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8876718.1"
                     /translation="MNREPAAVVTGASAGIGEAIARRLLDAGRTVVTMQRNAPPIEHP
                     AVRFERVDLSDMAATRDAARRVASTYDVRYLVNNAGANCPALLDDATIDELSYVTNIT
                     LGAAMLLIQAFVPGMREARFGRIVNISSRAALGKMGRTVYSTAKAGLIGMTRTVAIET
                     GAHGITVNAVVPGPVETKHWARGHPPGSQQRQTVVDMILVRRLGTPDDIARAVAFLLD
                     DDAGFITGQALFVCGGTSVTGTGGQ"
     gene            complement(17551..18894)
                     /locus_tag="VFO53_10360"
     CDS             complement(17551..18894)
                     /locus_tag="VFO53_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8876719.1"
                     /translation="MERTSKGEGRLSYVLGARMIDCTGAAPVQDPVIVVDGTRIAQIG
                     TRKTVKVPDGADIVDCGNATLIPGMLDIHLHTMMFNCLTFHNFRVAQWEITPELQQMY
                     GLFHAQLCFDMGFTTLRDMGLQTYRGLLVREACAIRDAIDMGIVEGPRMLIAAFTTIT
                     GSHLDLIQPRAAERLGFQNADGPWELRKLARTNLLHGADVIKTCASGGGGTDKEEPDI
                     RNMTQVELDAIVDEAHAFHKICAVHCFTPQSQRMALAAGADTIEHMVFSDDETIGMIR
                     EKGTYVTPTLSHRTDHAIEMRREQGTSQFVLKKMKSIQENCFDTFQKMHAAGINIAMG
                     TDMGFDPEMGTNAAELEIYVNLGMSPMDALLTCTRNAARAIKLDRQIGTLEAGKLADV
                     VAVAGDPLADIRCLQDKKNIQIVMKEGKVYADRRPGHAKSVVNAEPGSWKIPDYL"
     gene            complement(18915..20084)
                     /locus_tag="VFO53_10365"
     CDS             complement(18915..20084)
                     /locus_tag="VFO53_10365"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET8876720.1"
                     /translation="MTDAGAGRVPVVLVTGFLGSGKTTLVNRLLKSHRYARAAVVVNE
                     FGEVSVDHLLVDAPRRRTRIIDAGCVCGHVHEEVATSLLDLLDHRASHADAQFDRVLI
                     ETSGLADPVPIIQILLADAAVSAAFELRTVVTVCDGVHGVAQLGTHEESMKQAAVADV
                     LVLSKRDLAGDDALASLSRRLASLNPGARQCAATYGELDLSLLDAGGYAIDARSADAK
                     TWLCDSRYASATPAASQDPSIRTFGLVHDGEITVPGLVLWMNLLAGFRGAGLLRVKGI
                     LNVEGQPYAVHAVQSVLSEPVPLDRWPDGDDRRSRLVFITRCIAPEDVKRTFATLAFE
                     GGREQRNLTLRPETYARFKQTMEVFRAGSRQASKAPASRDSASARVAASWVTPNT"
     gene            complement(20244..21158)
                     /locus_tag="VFO53_10370"
     CDS             complement(20244..21158)
                     /locus_tag="VFO53_10370"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET8876721.1"
                     /translation="MEIRLLRYFSAVAEARSFTRAAQTLGISQPTLSHQIRKLESDLG
                     TILFERAVRNVTLTSAGSTFKPFCDRTLKELDSGVLAISDLEGLMRGTLRMAVFHSFA
                     NSGLGPVFARFALLFPGVRIEARLLPRMEMERQLIAGNLDLALAYVSEDKEHIVAERL
                     FDEELVLVVGARNRIAAGPRLTMRRIVDFPLVLLTPEFAARQFIDRFFASRNLQPKIA
                     IEMNAVDPILAVVRDSDLATVISEGAVGNEQVARVVRLVDPVPRRALAVLWRRGGYRS
                     TAALRLAEMIRANYRGATGTKGPKRRRG"
     gene            21480..22436
                     /locus_tag="VFO53_10375"
     CDS             21480..22436
                     /locus_tag="VFO53_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET8876722.1"
                     /translation="MKAMQRALAAAAAIAASVFVSIAPAQEKYPSRPVEIIVPWGPGG
                     GADQLARLTAKLTEPVLGTSFPVINTPGATGATGMAKLLAEQADGYAMAIYIADTNAL
                     LAGPNPRWKLGDITPIAVMMQVPSFLFVAENSPIKDWAQFEKEARAKPKTLKVATLGF
                     GSVDDMTLQFLASKGITVVEVPYAKPSERYVSVIGGHVDALYEQAGDVRQFILNKQIR
                     PIVIFGKERLAAFKDVPSSYELGYHIALPQFRSYVVKAGTPPERVKILSDAMAKAAAT
                     PEYQKFLEENFSARDSFIPADKAEPYLAAQVADMKAAMVQKQ"
     gene            22458..23003
                     /locus_tag="VFO53_10380"
     CDS             22458..23003
                     /locus_tag="VFO53_10380"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HET8876723.1"
                     /translation="MFGKLASLAPYVALCAVAGYLYRDAGAFAAAARPGQLGPDFWPR
                     AVLVLLIVVCVCEIVRRIAFERAAHDDSSSAYASGNEDAQAASEDRFAWCLAGGIALT
                     IAYVLALDWLGFFVATALYLALFMVVGRYRRTRVIVSASVLGSLAFVYVFMKIVYVSL
                     PLGRGPFKALSVWLLALLGVR"
     gene            23017..>23564
                     /locus_tag="VFO53_10385"
     CDS             23017..>23564
                     /locus_tag="VFO53_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HET8876724.1"
                     /translation="MDETLHQLGLGFLHLLTLPNAASLVVGLVVGMLVAVLPGLTLVM
                     GVVLALPFTYGMSVTPSIILLTAMYLSGTYGGAFTSILFRIPGEPLDVPLLWDGYPMA
                     RQGKPAQALGWTLLAALWGGLITCVSAVVLAVPFAHFALRFDSPEYFAVVLLGLSGVV
                     ALGGASMAKALISLCIGLLLATV"
BASE COUNT         3953 a         7989 c         7730 g         3892 t
ORIGIN      
        1 tcgtgcacga cgcggctcac gtgcacgccc tcgggcggcg caaggttgct gacgttgagt
       61 cgtcccgaaa catccgtcca ggtgtagacg tccgcatgcg cgagcggcac accgagtgcg
      121 ccggcgagca cggccgacac cgcggtacgg agaaggaacg gatgcatgat cggactcgcg
      181 actgccttcg gcaattctat gccgctgcgc cgagcgtggg tagcatcacg atttgagccg
      241 gtaaccggtc cggaagatcc aggcaacgat cacgaggaac acgacgagaa acgcgatcgt
      301 catcgcgagg ctaacagcaa tatcgacgtc ggcaatcccg tagaagctcc atcggaagcc
      361 gctgaccagg tacacgaccg gattggcgag cgtcgccttc tgccagaacg gcggcaacat
      421 gtcgatcgag tagaagctgc cgccgaggaa cgtgagcggc gtgacgatca ggagcggaac
      481 gagctgcagc ttctcgaagc cgtcggccca gatgccgatg atgaagccga gcaggctgaa
      541 ggtgacggcg gtcagcacga ggaacagcac catccagacc gggtgctcga tgtgcagcgg
      601 cacgaacagc gccgcggtgc cgaggatgat caatccgagg atgatcgact tgctggcggc
      661 ggcgccgacg tagctcatga cgatctcgaa gtacgacacc ggcgccgaca agagctcgta
      721 gatcgtcccc gtgaacttcg ggaaatagat gccgaacgac gcgttcgaga tgctctgcgt
      781 caaaagcgac agcatgatga gccccggcac gatgaacgag ccgtagctga cgccgcccac
      841 ctgctggatg cgcgagccga tcgccgcgcc gaaaacgacg aagtagagcg acgtcgagat
      901 caccggcgat acgatgctct gcagcagcgt gcgccgcgtg cgtgccatct cgaagcggta
      961 gatcgcgcgg accgcgtgca ggttcatggg cgctgctcgt ggacgaggct cacgaagatg
     1021 tcctcgagcg agctctcgct cgtctgcagg tccttgaacc ggatgcccgc caggtcgagc
     1081 tgctcgagga gcgatgcgat gccgctgcgc acgctgtccg cctcgtacgt atacgtcagg
     1141 tcgttgccgc cgttgacgag ctcgagcttg tagccggcca gcgccggcgg aatgcgctcg
     1201 agcgggtcct gcaggtgcag cgtgagctgc ttgcggccga gcttgcgcat cagctcgacc
     1261 ttgtcgtcga cgaggatgag ctcgcccttg ctgatcacac cgatgcggtc ggccatttcc
     1321 tcggcctcct cgatgtagtg cgtcgtgagg atgacggtca cgccgccgct ccgtagcgag
     1381 cggacgagcg accacatgtc gcggcgcagc tcgacgtcga cgccggccgt cggctcgtcg
     1441 aggaacagga tgcgcggctc gtgcgcgagc gccttcgcga tcagcagccg ccgcttcatg
     1501 ccgcccgaaa gcgtcatgat gcggctgtcc ttgcggttcc acagcgaaag atcgcgcagc
     1561 actttctcga tatgcgccgg atcgggcggc ttgccgaaga ggccgcggct gaacgacagc
     1621 gcgccccacg gcgtctcgaa cgcgtcggtg gtgagctcct gcggcacgag accgatcaac
     1681 gcacgcgttt cgcgatagtc gcgcccgatg tccttgccat cgacgagcac cctgccctcg
     1741 gtggcattga cgatgccgca cacgatgctg atgagcgtcg tcttgccggc gccgttcgga
     1801 ccgagcagcg cgaaaatctc gccacgccgg atgtcgagat cgacacgctt cagcgccgcg
     1861 aagcccgacg cgtaggtctt ggacagattc gatatcgaaa tgatcggcgg catggcctcg
     1921 gccggcgcgt gcggagcgcc attgtcgcag ggtggcgtcg aggctgcggg cgacaccgaa
     1981 gacatcatcc gagcgccgac ggcacgagag cgagccattt cctgccgcgc gtgcgctcgg
     2041 ccacgaacgt atcgcgcacc gcgttcaacc gatacgtgag gtaagcgcga tggcgcacgt
     2101 tcgacaggcg ctgcggcttg aacacgcacg cattgatgcc gtcgcagcgc gccgattgga
     2161 cgagcaaccc gttctggtcc tgctgatgca ggaagcggcc gatgcgctgc gtgaacacgt
     2221 agctcgtacg gctggtgagc gcgggatgct tcgcttgctt gccgcgcagg tcgatcagca
     2281 gcgcatcgca gcgcacgtcg aagagacgcc gctcggtgac gacgtcgcga tcctcgcccg
     2341 catagctgtc gagcacgaag cgataccagt gccaggccgt ctcgcagacg gtggtttcaa
     2401 ccgacatcga tccgtaccag acgccatact cggtgccatc ggaaaagcgc gtggcctgcc
     2461 agtgcgacgc atcgaacgtg taagtgatga cgctgccgta gtcgaagggc cgcgtgacca
     2521 ccggcgcgtc ggtcgcgatg cgcgcggccg cttcggcggc gatggccacg tcccaatcgg
     2581 ccggatcggc cgaaaggtcg tcgaacaggt gctgcgattg gcgaatcccc ggaatgttgc
     2641 gcacgaggtc gccgtggaag tcgacgttcg cgtcgaccag gtccgcctgc atccgctgcc
     2701 cttgcggccc ggcggcgcgt cagcttccgc gctggaagtc gaggtagcgg cgcagcgcga
     2761 gcagaccctc gaagccatgc tcgagaacca tgtcgaccgg acgcgtgccg agacgccgat
     2821 tgggctgcgt catccagcgg taggcgagat cgcgatcgtg cggaaacaga atccgcagcg
     2881 acttgtggat gccgagcagg tgcccggcgc gatcgagcag gtcgcgattg tcggcaagcg
     2941 gctcgccgct gcgatagcgg ccgagcgtgc tgcggctgcc ggtggaaagg ccgagcacat
     3001 cggcctgctc ggcggtggtc agtttccagt gctcgagcag cgtcgtgacc atcgccgcga
     3061 ggcggccgcg tgcctcgcgg ctgcccggct cgatggcgac gcgcttgcgt gcgacgactc
     3121 gcatcggcgg accccgctgt ctttaatggt acgtttattg taccagaagc gacagcgcgg
     3181 tcgaagcccg gcgacatccg ccatgcaccg gcctcatcgc cgcggcgggc atgccgctca
     3241 ggatgcgcgg ccgaattgcg cggcgagccg ctccgcgcca cgcgcgagga tcgcaacgtc
     3301 gacgccgacg gcggtgaaca gcgaccccgc ctcgatgcaa cgcttcgcga gttgcgcatc
     3361 gccgacgagg atgccgggcg ccttcccgct gcggcggatg cgccgcagcg catcctcgat
     3421 caacggcacc accttcggat tcgccacctc cccggcataa cccatcgagg catgcaggtc
     3481 ggcgggaccg atgaaaatgc cgtcgatttc gtcgatctgt gcaatggcat cgatgtgctg
     3541 gagcgcctgc tcggtttcga gctgcacgag caggcagagc tcctcgtgcg cgtgttgcgc
     3601 gtaatcgctc acgcggccga agcgcgtggc acgcgtcgct cccgccacgc ctctgacgcc
     3661 ggcgggcggg tagcgcattg ccttggcggc cgcacgcgcc tcgacggcat cctgcacgta
     3721 cgggatcagc aggctttgcg caccgatgtc gaggacacgc ttgatcgtca ccgtgtcgtt
     3781 ccacggcacg cgaacgacgg gcgtcgacgg atacgccgca accgcctgca actgcatcag
     3841 caatgactcg agatcgttcg gcgaatgctc ggtgtcgagc agcagccagt cgaaaccggc
     3901 gccggcaacg acctcggcac tgtagtggct ggacaggctg caccacaggc cgatctgcga
     3961 ctggccggcg gcgagcgcgc gcttgaaacg attgattggc aagttcattc cggctccgcg
     4021 atcggttgac ggtgcgttgc gccttgcggt gcgatcagcc ggggtcggca cgcaacccgg
     4081 ccgcctcgat gccggcgatc gcgcaggcct cgtcgtggtc ggactggtcg ccgctgacgc
     4141 cgaccgcgcc gacgacctca ccgccgatat cacgaacgag cacgccaccc ggattgggga
     4201 tcaaccgccc gccgcttgct gccgccagcg ccgcaaagaa gcgcgggttc tgctcggcct
     4261 tgcgtgcgag ctcgcgcgat ccgagcccca tgccgagtgc gccccacgcc ttcccacgcg
     4321 cgatgtcgac tcgaagtatt ccgcagccgt cctctcgctt cagcacgatg tcgtgtccgc
     4381 cagcgtcgag gacgacgacg gcgagagggc gaagcttcgc cgcacgcgcc gaagcgagta
     4441 ccgcgtcgac gatcgtgtcg gcggcttcga gcgcaagcgc gtatccgggg ttgctcatgc
     4501 ctgcagcgtc ggctcgccga tcttttcgtc agtgggcgcg ctcctggccg ccgcttgcac
     4561 ccccggcatc gccgcgcgca tcgccaccac ccggtccctg ggaatgtcgt tgagccgcat
     4621 cgccgcacgc atcatgaccg cggcttcatt caccatatcg tcgatgagaa cgaattcacc
     4681 ttccttcgct cgcagatcgc ccgcgcgtgg tccggccgtc gggctcgtgc cgccggagcc
     4741 gtagatgcat gtcgggatgc ctagccggtt tgcatcgatc gcgtccgtgc agtacggagg
     4801 gcagaacctg gcaggcacgt cggcacccgt ggtttcatgg tgcgcgtcga cgacggactg
     4861 gacgacagca tgggtcgcgg agatctcggt tcccggccgc gccatgtagt actcgatgtc
     4921 gaccggtcgc tgcagcttct gctgcgcaat cctcgtgacg acctcggtaa actcacgctg
     4981 cacgtcgatc gggtagcgat tcggagggaa ccggatgtcc acgtacagct gcgcgagatt
     5041 gcacgtgcgc gcagctcgcc acgggagacc gccccgcatg caagcgcgat tcaccgcggg
     5101 cacgatgccc tgataggtgt gctgcttctt gtactcggca atccacgcgc cgagctcttg
     5161 ccacagaagc cacatttcat cgagcgcgct aagcgtcgag tcgtgaaacg cgctgtgcgc
     5221 gaaatcgcca tgcacgctga ccttggccca ggtcgtgccc atgacgccgg tcgatggcac
     5281 ctgcgcggtc ggttcgccga ggaaatgaaa atcggcggtc acgccatgga tcagcatgta
     5341 ccgcaatccc atgccataac cgtgataatg cttgccttga aactcgtcga tcgccgccat
     5401 ctcgatttca ccggcggtgc cggcgatcac cagttcgcct ccgagcctga cgcccgcgcg
     5461 gcgcatcgca tcgatgcccg caatgtacga gacgaaggcg tttttcatat tgcagatgcc
     5521 gttgccccag atgcggtcgt cgtcgagcac ggcgtggttc ttccatccct tgccttgcag
     5581 gtagtcctcg tcgccacgca ccgacgtatc catgtgcccg ctcatcagga tcgtcggccc
     5641 cgccccgcta cccgccagac ggccgatgca gttgtagcga tcgtcgtaga tgttctgcag
     5701 ctgcgtcgca cagccgacct ccttcaacgc gcgctcgagg gcgcgcgcca tgttgccttc
     5761 ttcgccggtc gcgctcgcag tgttcacgag ctcggtggcg agatcgacaa ccagctttcg
     5821 atcgacgaga ctaagaagct tttccgcgtt cacgattgga ccccttcaat gcgcagcgcg
     5881 ctttcgcgct cgcgcggttg cctgctgtcg ttattgttgg agagaacagt gtctggtcgc
     5941 acgcggccta cacatcgccg agaaattgca tgtagcgcct catgctcctt gccagtaccg
     6001 ccctgaagat gcggtcgacg atgagaccga cgatgccgag cgatagcagt ccgacgaaca
     6061 gctggtcggt ctgcagcagc atctgcgaat tcatgatgag gaagcccagg ccttcgcttg
     6121 caccgatcag ttccgcggcg acgatgccca tgaatgaaaa gccaagcgcc acccgcatgc
     6181 cggtcagcac atagggcact gcggacggca ggatgatctt gatgaagacc tcgtactcgc
     6241 tcgcgccgag gcagcgcgct gcgcggatgc gggtaaccgg cgtacgcgag acgccggccg
     6301 cggtattgag cagcacgatg atcaccgtgg cgtagatgat gacgaagaac ttcgacaatt
     6361 caccgattcc gaaccacagc atcgccagcg gcacgatggc caccggcgaa atgggccgga
     6421 tcagctcgag catgggatcg gcgagctcgc gcacgattct cacgcgtccg atcaacgccc
     6481 cgacagcgat gccgacgacg caaccgacga cgtagcccac gagcacgcgt cgcatgctca
     6541 cggcgatgtg catgaacagc tcgccggtca gcatcatccg ccaggccttg ccgagcgtct
     6601 gttcaggcgt aggaaccagc gccgggttga agagggtgcc gaccgacaag tgccacaaca
     6661 gaagcgcgaa gccgatcgag atggttccca tcagcagctt gcgccccgct gcgggaagcg
     6721 cgatgcgtcg cacgcccggg atgcgcactc tggctacggt cgcggcgcgg tcggcgggca
     6781 gcgtctctgc tcgtctcacg ccagtgcccc ttgtgcgcgc cgccccttgt gcacctcttc
     6841 cagcagcatg ccttccaata tcttcacgag ctcgacgaac tgcgcatcgg tcgttcgtct
     6901 tggacggggc agatccacgg tgatctcgtg cctgatcgtg gcctccggtc cgctggtcat
     6961 gaccgcgatc ctagttccga ggatcagcgc ctcgacgatg tcatgcgtga cgtagagaac
     7021 cgtcttcccc gtctcttgcc agatccgcga catctccgtc tgcagcaggc gcttggtaat
     7081 cgcatcgagc gcagcgaacg gctcatccat caacaggatg tcgggttcca tgatcagcgc
     7141 ccgtgcgatc tggacgcgct gcttcatgcc acccgagagc tcgaacggga acttgtgccc
     7201 gtgatcctgc agacccacca tgtgcagata gcgcgccgcg ctgccggcgg agccgcgagc
     7261 tcgcgcagcg cgcaggcgcg gcccgaacag cacgttctct tcgacgtcga gccacgggaa
     7321 aagggcttcg gcggcatcct ggaagatcat cgagcggtcg acgccggcgg cgaagatcgg
     7381 actcgtccac tcgaccccgg cgtcctcgcc gacgaaaacg atttcgccgc tcgtcggcgg
     7441 atcgaagccc gcgaccatac cgagcaacgt gctcttgccg cagccgctcg ggccaaggag
     7501 acaatagaag tctccgcggc gcaccgaaac gtcgatatca cgcgtagccg cgtattggcc
     7561 acctcggctg tcggcgaaca ccttgccgac gtgccggaac tgcagcgcgc tccattcgcg
     7621 cgtccggctc ttacccgcgc ggtcgtgcgt agcgccggaa ttcatcggga accacgctca
     7681 cgcgacgtag ctggcgccga gtttttgcag atggcggctg tcgaacatcg tctgccagtc
     7741 gatcctggca ccgatgaagt ttccgacccc gtccatgtca gcgcggaagt tcggcgtgta
     7801 ggcgatgttc cagtcgatgc gctcgatcat gtcgcgcgtg atcttgacgt cgtcgaaccg
     7861 aatttgcgcg gcgacgatct ttgccgcctc gtcgaggttg gcgggaatcc atcggcccgc
     7921 cgcgtcgagc cccctgagga acgcaagcaa ctcagccccg tgcgccgcgg cgtattgctt
     7981 gctggtcgaa acggcttccc attcctcgaa atatcccttg ccggtggcga ggatgtgcgc
     8041 gtccttcacg gcggcagtcg cgcgccagcc gaacggctgc cagatgacga acgcatcgat
     8101 gtcgccgcgc gcaagacccg tcaccatctc ggccggccga agattgatca tctggatgtc
     8161 gccgacattc atgccggcct tcgacaggta gagcttcgtg aagtactccg acgtcgagcc
     8221 gagcaggacg ccgaccttcc tgcccttgag atcgcttgcg ttcctgacat ggccttgcgc
     8281 aacgatgacc gaaagcgagt tgtaatgcgc ctgcgggcag aggccgatga agccctgctg
     8341 ccagagccgg atcgatggca ggtcgcctgc gacgagaatc gagcccttgc cggtgcggaa
     8401 cgcgtctgcc gcgacggacc cgctcgcgaa gttcgtgatc ttgaccggca atccttcctt
     8461 ctcgaagaat cccttgtcgg cgccgacgat gatgtttccg aagtgaggcg tcgtgccgat
     8521 gtagatgtcc atcggctgga gctttgcctg cgctgtcgcg agtctcggga tgccgcaagc
     8581 ggcgagcgcc gctgccgtgc cgagcgtctt cagcacgttg cgccgtgaat gatctgccat
     8641 gtgctcctcc tcggtatgca ggcccgcgat cgaacgccgc gggtcgccgg ttctacgctt
     8701 gcgtcgacac ccctctttga cggcgagaca atagcgcgcc ggaaggcata gagcaaatgc
     8761 actcactcta tgttttgcat gcacgacatc tatgagcacg ggcatggcat cgcaacgcga
     8821 acgccgcgtg ggacaagagc ccggacgaac ctaaagaatc tttccgggat tcatcaggtt
     8881 caacggatcg aacgcctgct tcagcgtgcg catcatcgcc agctcgacgc cgctcttgta
     8941 ccgcgcaagc tcggcgcgct tcgcctgtcc cacgccgtgc tcggcgctga agctgccgcc
     9001 gagcgcgtcg acgacgtcgt agagcaccgg gtagagcggc ttcgaccagt cgctttgcgc
     9061 ttccggcgcc gcgggcggca gcacgttgaa atgcaggttg ccgtcgccga cgtgcccgaa
     9121 gcatacgatg cgaacgcccg gcatggtgcc gaggacggcg tcggtggcgt cggcgatgaa
     9181 gcgcgggatg cgatcgaccg cgaccgcgat gtcggagcga agcgcgggcc cggcgccgcg
     9241 aatcgcgtcg gggatgctct cgcgcaagcg ccacagcgtc tgaccctgcg cgacggtctg
     9301 tgcgatcacc gcgtcggcga cgctgccctg ttcggcgctg tcggcaagca gcctttcgag
     9361 atcctcgcgc agcgtgtcgc cgccgtaaga accggcaacg tcgagcagca cgtaccacgg
     9421 gtgcggctct gcaaacggat cgcgcgtgcc gtggatatgc gcacagacga gatcgaggca
     9481 cgcgcggccg atcagctcgt acgcgatgag cgcgtcgccc tggcgctcgc gaatggacgc
     9541 aagcagcgtc accgcgtcct cgggctcgcg caacgcgacg agtgcgctca cccgttgggc
     9601 gggccgcggg aacagcttca gcaccgcccc ggtgatgatg ccgagcgtgc cttcggcgcc
     9661 gatgaagagc tgcctgagat cgtagccgcg attgtccttg cgcagcgcgc gaaggccatt
     9721 ccagacgctc ccgtcgggca gcacggcctc gagcccgagc acgagatcgc gcgcgttgcc
     9781 atagcggagg acgttgatgc cgccggcgtt cgtcgacagg ttgccgccga tctggcagct
     9841 tccctcggcg gcaaggctca gtgggaagag gcgaccggcg gccgcggccg cttcctgtac
     9901 ctgcgcgagc aggcatccgg cctcgaccgt aatcgcatcg ttgcgcgcat cgaccgcgac
     9961 gacgcgattc atccgccgca acgccaccac gatcgcggtg cggcttgcat cgggaattgc
    10021 gccgccgcac atcccggtgt tgccgccctg cggtacgacg ggcacgcgtt cccgacgaca
    10081 cagacgtacc acgtccgcga cttcgtcggt gctcgcggga cacgcgatgg cgagcgcttc
    10141 gccgcgaaac gcgccacgcc agtcgctgac gtagggcgcg acgccggccg cgtcgacgag
    10201 tacgttggcg ggaccgatgc agcgacgcag cgcatcgatg aaagccgcgc tagagctcga
    10261 cacccgctcc gtcctcccac aaccgcacga cctcggcctg gtcgcgcccg tggccatagc
    10321 gctgcgccgc ttcccgccac agctcgtgcg tcgcctcggc caccggggcg cgcgcaccga
    10381 tggcacgtgc cagattcgtg acaatccgga cgtccttcgc gatcagcccc atcgccatgc
    10441 cggtgccgaa gcggcgcgtc atcacctgcg gaacgatctt cttttcgagc ggatgctgcc
    10501 ggccggcgca catcgccgcg agcgcctgcg ccatgaaggc cgactcgagg ccgaacttct
    10561 tcccgatcgt catcgcctcg agcagcgcga tgaaggtcgc tgcgttgacg aaattggcaa
    10621 tcgccttcag cgcatggccc gacccgcttg ccccgcaaag ccacgtgcgc cggccgagcg
    10681 cggcgaacag cggcgtgcag cgctccaccg cgtccggcgc gccaccggcc atcacgtcga
    10741 gcgtgccgtc gcttgcgaac ggcacgccgc ccatcaccgg cgcatcgacg tacgcaagcc
    10801 cgacgcgtgc gaattcggcg gcgatggcga gcgtcgactc cgggctcgac gtgcccatgt
    10861 cgatggcgat ggcgtcatta cggcgcagcg acgtcgccac gccgccgtcg ccgagcatga
    10921 ccgcgcgcac ggcaccgtcg tcgggcagca tcgcaatgac gaacgccgcc tgatcgaccg
    10981 ccgcggcgat cgacgtcgcg ccgataccgc catgcgcgcc gacgaaacgc tcgacaagat
    11041 cgccgcgcaa gtcgtgaacg cgaagcgagg cgccgcgcga cgcaagccgc gccgccatcg
    11101 gcgcgcccat gtgcccgagc ccgatgaacg cgatcggacc gatttcgtcc aacgtagccg
    11161 tcatcgtccg gcccccgtcg ccattcccca ccggttcacc gtcacttgct cgaggagctg
    11221 gaacacgacg cgctcgaaga acaggcccag cacgccgatc acgagcagcg ccgccagcat
    11281 gacgtcggcg ttcaggaact ccttcgcatc gaagatcagt tttccgaggc cccactgcgg
    11341 gcttgcgaga agctcgccgc cgatgacggc gatccatgcg cggccgaatg ccaggcgcac
    11401 gccggtgatg atcgacggca gcgcggcggg ccagacgacg tggcgaaaga gccgcacacc
    11461 gcgtgcattc atcgcctgcg cggcgcgcag ccacaacgga ttgatcgtgc gcacacccat
    11521 ccagacgttg tagagcagcg agaacgtcga tgcgtagacg acgatcagca ccgtcgcgac
    11581 attgccgatg ccgaaccaca gcacgacgag cggcacccat gcaagcgacg ggatcggcat
    11641 cagcaccgca agcggtggac gcaggatccg ctcccacagc ggatggcggc ccatcagcat
    11701 gccgagcgga atcgcgatgg cgagcgcgat cgcgagcccg atcgcgacgc gcaacatcgt
    11761 cgccagcgta tgccggaaga tcgagccgtc ggcgatcatc gcgacgagcg ccgcgacgat
    11821 cgtctcgagc ggcggcgtga tcgcctgcga gaagatgccg ctgcgcgcga aatactccca
    11881 ggcggcgagc aggagggcga tcgcgatcac gccgcgggcg aagggcacga cgcgcagcct
    11941 gcgcgccggc aacggcggca ttgcggcgat ggcgacgacg ggctgcctcg acaccgcggt
    12001 catgcgctca tcatgcccca gcgaacgaca gtcgccttct cgaggcgcat gaacagctgg
    12061 cgctcgagca gcatgccgac gatgccgatg accgcgatcg tcgcgagcat cacgtcggtg
    12121 ttgagaaatt gctgcgagcc gaagatgagc cagccgagcc cgcgcgcgac cgacatcagc
    12181 atctcgacgg caacgaggat ccgccatgcg gacgcgagcc cgagccgcag ccccgtgagc
    12241 acgtacggga gcgcgccggg caggatcacc ctggcgaaga tcgcgcgctc gcttgcgcgc
    12301 atcgcctgtg cggagcgtat ccagatcggc ttcaccgcct tgacgccctt ccacgtattg
    12361 atgatcacgg tgaacgacgt cgcgacggca acgagcagga tcgccggcac gtcgcccaac
    12421 ccgaaccaca gcacgaacag cggcgcatac gcgatgccgg gaatcggata catgaagctg
    12481 acgatcggca gcaacgcatc ctcgatccac tgcacgcggc ccatcaacag gccgaccacg
    12541 atgccggcga ccgcggcgag cagaaagccg aacagcagcc ggaagagcgt cgccgcggcg
    12601 gccgcgagca tcgtgccgtt ctcggtcagc cggagcagcg tttcggccac ggtcgcgagg
    12661 ctcggaaaga gtttcggcgg gaacacgccg gcccacaccg ccgcctgcca tgcgagcgcc
    12721 atgacggcga aagcgagcga tccggcggcg accgcgccga gccgcgacat cgcacggctc
    12781 gaattcatgc ccttttcgcc tgcgcgagca cgctttcgtc gagcgcgcgg ttccagtcgg
    12841 gcacggcggg gatctgtttc tggccgagca gcacgcgtcc ctgctcgttc aggtagtcgc
    12901 gcagcgccgg cgtgaattcg ggtttcacgt cgagcttcgc gagcatccgc gtgaacgcgg
    12961 cctccttgac gtcgtagccc tgtgccttga attcgttcca gacgatctcg accgcacgct
    13021 tcgggtgttc gttgacgatg cgcaccgcgg cgagccagcc gcgcaggaac gcgaccacgt
    13081 cgccgcggcg gctttccagc acgggccggt tgatcgcgac gaacaccggc tgcaagtcga
    13141 actgcccgta gtcgacgagc acggtgccga ggccctcgat ttcggcgacg gatggaaaag
    13201 gctcgacgcc cgcgaaggcg tcgaccgatt tgccgaccat cgccgcgatc tcgttctggt
    13261 gcggaatgtt cgcgatctgc acgtcgccct tcgcgaggcc gaattttggc aggatcttgt
    13321 tctggaacac gtagtccgtc gacgagccca gttgcgaggc gacgcgcctg cccttcagct
    13381 cggcgaccga ccggatgccg ctgtccttgg cggcgacgac ggcgttcgtc ctgccggcat
    13441 acatcgccac cgcgatcgcg acgatgtcgc ccttggccgc acccacgacg aacggcgcgg
    13501 cgccgagcac gccgatgtcg acgcggccgg cgatcatcgc gtcgcgcgtc gacttaccgc
    13561 cgtcgaagcg aaacggcttc accttcgcac ccgccttctc gaacgtcttc tcggcttcgg
    13621 cgaccatcgc caccgatccc cacgacgtgt tctgaaagcc gaagttgagc ggcggcgagt
    13681 cggcgcgagc atcgtgcggc agtgcgagcg cgactgccgc agcgccggtc gacgtgagga
    13741 acgtgcgccg ggtgatcgat cggatcatgt ctggccttcc ttcgcgcagc acgcgtggcg
    13801 agctcatgct ttcgtcgcct gcgttagcac gctcgtatcc agaatccggt tccagtcggg
    13861 cacgctcgaa atctgcttct ccttcacgag cacgtcggcc tgctcgtcga ggtactgctt
    13921 gaggttcggt acgtactggg gcttgacctc gagcttcgtg agcatcagct tgaacacggc
    13981 ctccttcgtg tcgtagccct gcgccttgaa ctggtcgaag acgatgcgca ccgcgcgctg
    14041 cggctccttg ttgacgatgt cgaccgcggc gagccagccg cgcatgaacg ccacgagctc
    14101 gttcttgcgc ttgtcgagga tcggcgcgtt gaccgcgagg aacaccggct gcatgtcgaa
    14161 ctgcgaatag tcgacgatcg cgtggccgag gccctcgacc tcggcgaccg acgggaacgg
    14221 ctcgacgccg gcgaacgcgt cggccgactt gccgacgatc gccgccacct ggttctggaa
    14281 cgtcacgttg acgatgtgca cgtcgtcctt cgtgaggccg gacttgggca ggatcttgtt
    14341 caggaacacg tagttcgtcg cagagccgat ctgcgacgcg acgcgcttcc ccttgaggtc
    14401 ggcgatcgtc ctgattccgc tgtccttcgc cgcgacgatc gcgttcgtct tgcccgcgta
    14461 catcgcaacg ccgatggcgc ccatgtcgcc cttcgccgca ccgacgacga acggcgtgcc
    14521 gccgagcacg ccgacgtcga tgcggcccga gatcatcgcg tcgcgcgtct ccttgccgga
    14581 gccgaagcgg tggaacttca cgctcgcgcc ggccttgtcg aacgtcttct cggcgtcggc
    14641 gaccatcgcc accgtgcccc acgacgtgtt ctggaagccg agattgagcg gctgtccggc
    14701 ctgtgcctgt gcgcgcaacg gccatgcgcc cgcgagcgcg gccgcgccgg cggccttcag
    14761 gaaatcgcga cgctcgatgc gacctttcga catgacgtcc tccttcgatg agtgttgccc
    14821 ttcctgcagt ccgcccgcgg cggctctcgt atttcagtgc atgccgagct gcgccagtat
    14881 ccggcgctgc gcgtcgacga acggcggcga cagcggatcg cgcggccgcg gcaggtcgag
    14941 cgaaatttcc gccttcacgc gccccgggtg cgcgtcgagc acgacgacgc ggtcgccgag
    15001 gtatacggcc tcggcgacgt tgtgcgtcac gtagacgacc gtcttgcgcg tctcgcgcca
    15061 cacgtcggtg acgagccgct gcatctcgtc gcgcgtcatt gcgtcgagcg cggcgaacgg
    15121 ctcgtccatg agcagcacgc cgggactgta ggcgatggcg cgcgcgattg cgacgcgctg
    15181 ctgcatgccg ccggatagct gatgcggata ggcgtcggcg aattcctgca ggccgacgag
    15241 gcgcaggaat tcgagcgcga cgcgcttgcg ctcggcggca ggcatgttgc gcatctcgag
    15301 cccgaactcg acgttgcgcc gcgcggatcg ccacgggaag agctgcgcga agtcctggaa
    15361 cacgacgccg cggtcgtgct cgacggcgtc gaggcgcgcc ccgccgatca gcacctcgcc
    15421 gccggtgggc cgcaggaaac cggcgatcat gttcatgatc gtcgtcttgc cacagccgct
    15481 cggcccgagc aggcacagaa attcggattc ggcgaccgta aagctcacgt cgtcgacggc
    15541 gagcacctcg cgcgcctcgg tgcgatagcg aagcgatacg tgcatcaggt cgatccgttg
    15601 cgccatgacg ttcgtgaccc tcgctgtgtc agccgcccgc catggcattg gcgtggcgcg
    15661 cggcgtcgcc cgcctgcgtt ccgcgcatcc ccgccgccgc aagcacctcg tcgagcgtca
    15721 cggcggcgcc gtcgcgcgac gccgagacga tcgccgcctc gaccgcggcc agcgcctggc
    15781 aaccgttggc ggccgacagc tcgcatgtcc cgtcgccgcg caccgcatcg gcgaactgct
    15841 cgagctcgtc gcgaaacatg ttgcccggcg gcacgggaat gcgcgtgcgc gcttgcgggc
    15901 cgtcgccgcg cgcctgccgc gccagcaccg cgcgctcgtg caggcgcgaa ggctctgccc
    15961 accgcgactg gtcgaccgca tagtcgacgc tcgcgcggtc gcccgtcgcc tgcacgcgat
    16021 attctccggg gctcgtccag ctcgtgacga tgcttccgag cttgccgtcg gcgaagcgga
    16081 tcgacacgac ccactggtcc tcgacctccg cgccgaccgg cgaaaggcgc gcgctctgcg
    16141 cggaaacggc tgcgaggtca ccgcccagcg cgcgcagcgt gtcgaactgg tggatgccga
    16201 tctgggacag cggtccgccc ggcgcgctcg cgctgtagaa gcgccagtcg ccgcgcgtga
    16261 gccggaggcc gcggtcgttc gagaacgtcg cctgcaaatg cgtgacgcgg ccgagctcgc
    16321 ccgccgcgat cgcgcgtgcg atccaccgat tgccggcgag catccgcgca caatggccga
    16381 cggcgagaac aatgccgtgc tgccgctcga gcgcgaccat cgcgaggccg tcctcgagcg
    16441 tgtgcgcgat cggcttctcg acgaacacgt gcttgccgca tgcggccgcc cgcgcggcga
    16501 gcgcccgatg ccggtcgttc ggcgcggcga tgacgaccgc ctcgatgtcc gcgtcgtccc
    16561 acacgcgatc gagggcgtcg accgcgcgga tgcccgttgc tgcggcgaac gcttcgcggc
    16621 gctcgccggt ggggctgaaa cagcggacga tctcgagccg cgagccgcgc gccgcctgcg
    16681 cgagcacgtt cgcccacgcg ccggtgccga gcagcgcggc gcgcaccggt cgcggcttcg
    16741 gcgagatcat cgtgccgcca cgcgcgatgc gatcgccgcg aacgggtcgc tcattggccg
    16801 cccgttcccg tgacgctcgt accgccgcac acgaacaacg cctgcccggt gatgaagccc
    16861 gcgtcgtcgt cgagcagaaa ggcaacggcg cgcgcaatgt cgtccggcgt gccgaggcgc
    16921 ctgaccagaa tcatgtcgac gacggtctgg cgctgctggc tcccgggtgg atgcccgcgc
    16981 gcccagtgct tcgtctcgac gggacctggc acgacggcat tcacggtgat gccgtgcgca
    17041 cccgtctcga tcgcgaccgt gcgcgtcatg ccgatgagac cggccttcgc ggtggaatag
    17101 acggtgcggc ccatcttgcc gagcgcggcg cgcgacgaaa tgttcacgat gcggccgaag
    17161 cgcgcctcgc gcatgccggg gacgaacgcc tggatcagca acatcgccgc gccgagcgtg
    17221 atattggtga cgtacgaaag ctcgtcgatc gtcgcgtcgt cgaggagcgc ggggcagttc
    17281 gccccggcgt tgttgacgag gtagcgcacg tcgtaggtgc ttgccacgcg gcgcgccgcg
    17341 tcgcgcgtcg ccgccatgtc ggaaaggtcg acgcgctcga agcgaacggc cggatgctcg
    17401 atcggcggcg catttcgctg catcgtgacg accgtgcggc cggcgtcgag cagccgccgg
    17461 gcgatcgcct cgccgatccc ggcgcttgcg cccgtcacga cggccgctgg ctcgcgattc
    17521 atgcgccgtg gcgccggcgt gtatgagcgc tcacagatag tccgggatct tccagcttcc
    17581 cggctcggcg ttgacgacgc tcttcgcatg gcccggccgc cgatccgcgt acaccttgcc
    17641 ttccttcatc acgatctgga tgttcttctt gtcctgcaga cagcggatgt cggcgagcgg
    17701 gtcgcccgcg accgcgacca cgtcggcgag cttgcccgcc tcgagcgtgc cgatctgcct
    17761 gtcgagcttg atcgcacggg cggcgttgcg cgtgcacgtc agcagcgcat ccatcggcga
    17821 catgccgagg ttcacgtaga tctcgagctc ggctgcattg gtgcccatct cgggatcgaa
    17881 gcccatgtcg gtgcccatgg cgatgttgat ccccgccgcg tgcatcttct ggaacgtgtc
    17941 gaagcaattc tcctggatgc tcttcatctt cttcagcacg aactgcgagg ttccctgctc
    18001 gcgacgcatc tcgatcgcgt ggtcggtgcg gtgcgacagc gtcggcgtaa cgtaagtgcc
    18061 cttctcgcgg atcatgccga tcgtctcgtc gtcggagaac accatgtgct cgatcgtgtc
    18121 cgcaccggcg gcgagcgcca tccgctgcga ctgcggcgtg aagcagtgca cggcgcagat
    18181 cttgtggaat gcgtgcgcct cgtcgacgat cgcgtcgagc tcgacctgcg tcatgttgcg
    18241 gatgtcgggc tcctccttgt cggtgccgcc tccaccggat gcacaggtct tgatgacgtc
    18301 ggcgccgtgc agcaggttcg ttcgcgccag cttgcgcagt tcccacgggc cgtcggcgtt
    18361 ctggaagccg agccgctcgg cggcacgcgg ctggatgagg tcgaggtgcg agccggtgat
    18421 cgtcgtgaac gcggcgatga gcatgcgcgg tccctcgacg atgcccatgt cgatggcgtc
    18481 gcgaatcgcg catgcctcgc gcacgaggag cccgcgatag gtctgcaggc ccatgtcgcg
    18541 cagcgtcgtg aaacccatgt cgaagcagag ctgggcgtgg aaaagcccgt acatctgctg
    18601 cagctcgggc gtgatttccc actgggcgac gcggaaattg tgaaacgtca ggcagttgaa
    18661 catcatcgtg tgcagatgaa tgtccagcat gcccggaatc agcgtcgcat taccgcaatc
    18721 gacgatgtcg gcgccgtccg gcaccttgac cgtcttgcgc gtgccgatct gcgcgatgcg
    18781 cgtaccgtcg accacgatca ccggatcctg caccggcgca gcgccggtgc agtcgatcat
    18841 ccgcgcgccg agaacgtaac tcagcctgcc ttcccctttc gaagtccgct ccatgatgcg
    18901 ttgcatgctc ctgatcaggt attgggagtg acccacgacg ccgcgacgcg cgcactcgcg
    18961 ctgtcccggg acgcaggcgc cttgctggcc tgtcgcgacc ccgcgcgaaa gacctccatc
    19021 gtctgcttga agcgcgcata cgtctcgggg cggagggtga ggttgcgctg ctcgcgcccg
    19081 ccctcgaatg cgagcgtcgc gaacgtgcgc ttcacgtcct cgggcgcgat gcaacgcgtg
    19141 atgaagacga ggcgcgaacg acggtcgtcg ccgtcgggcc accggtccag cggcaccggc
    19201 tcgctcagca cgctctgtac cgcatgcacc gcatagggtt gcccttcgac gttcagaatg
    19261 cccttgacgc gcagaaggcc cgcgccgcga aagccggcga ggaggttcat ccacaacacg
    19321 agccccggaa ccgtgatctc gccgtcgtgc acaaggccga acgtgcggat cgacgggtcc
    19381 tgcgaggcgg cgggcgtcgc gctcgcgtag cgcgagtcgc aaagccacgt tttcgcatcg
    19441 gcacttcgcg cgtcgatcgc gtagccgcca gcgtcgagca acgacaggtc gagctcgccg
    19501 tacgtggccg cacattgccg tgcgccgggg ttgagcgacg ccaggcgccg cgacagcgag
    19561 gccagcgcat cgtcgccggc gaggtcgcgc ttcgacagca cgagcacgtc ggcgacggcc
    19621 gcctgcttca tcgattcctc gtgggtgccg agttgggcga cgccgtgcac cccgtcgcag
    19681 accgtcacga cggtgcgcag ctcgaacgcg gccgacaccg ccgcgtccgc aagaaggatc
    19741 tggatgatcg gcaccggatc ggcgagaccc gacgtctcga tcagcacgcg gtcgaactgc
    19801 gcgtcggcgt ggcttgcgcg atgatcgagc agatcgagaa gactggtcgc gacctcctcg
    19861 tgcacgtgcc cgcagacgca accggcgtcg atgatccgcg tgcggcgccg cggtgcgtcg
    19921 accagcaggt gatcgacgct cacttcgccg aattcgttga cgacgacggc ggccctcgca
    19981 taacggtgcg acttcagcag gcgattgacg agcgtcgtct tcccgctgcc gaggaagccg
    20041 gtgacgagca cgaccggcac gcggccggcg ccagcatccg tcactccgcg gctccgtcgc
    20101 gctgcgtccg caccgatctc cagcgttgca tcactatcct cctgcgtcgt ccgatcgcgg
    20161 ccgggcacgc acctcgcggc aagcgtaaac cgggtaaatg aatagatcaa atgcaagcga
    20221 tgcatcgctt gcatcgatgc tatctatccg cggcgccgct ttgggccttt cgtgcccgtg
    20281 gcgccgcgat agttcgcgcg gatcatctcc gccagccgga gcgcagccgt cgatcggtag
    20341 ccgccgcggc gccacaacac cgcgagcgca cgccgcggca ccggatcgac gaggcgcacg
    20401 acgcgcgcca cttgctcgtt gcccacggcg ccttcggaga tgaccgtcgc gagatccgaa
    20461 tcgcgcacga ccgcgaggat cgggtcgacc gcgttcatct cgatcgcaat cttcggctgc
    20521 aggttgcggc tcgcgaagaa gcgatcgatg aactggcgcg cggcgaattc cggggtcagc
    20581 agcacgagcg gaaaatcgac gatgcgccgc atcgtcaggc ggggaccggc ggcgatgcgg
    20641 ttgcgggcac cgacgacgag gaccagctcc tcatcgaaaa gccgctcggc aacgatgtgc
    20701 tccttgtcct ccgaaacgta ggcgagcgcg aggtccaggt tgccggcgat gagctggcgc
    20761 tccatttcca tgcgtggcag aagccgcgcc tcgatgcgaa cgcccggaaa cagcagcgcg
    20821 aaccgtgcga agaccggtcc aagccccgaa ttggcaaacg aatggaacac cgccatgcgc
    20881 agcgtgccgc gcatgaggcc ctcgagatcg gagatcgcca gcacgccgga atcgagctcc
    20941 ttcagcgtac ggtcgcagaa aggcttgaac gtcgaaccgg ccgacgtgag cgtgacattg
    21001 cgcacggcgc gctcgaagag gatcgtgccg agatccgatt cgagcttgcg gatctggtgc
    21061 gacagcgtcg gttgcgaaat gccgagcgtc tgcgcggcgc gcgtgaagct gcgtgcctcg
    21121 gcgaccgccg agaagtagcg gagcagtctg atctccatgc gcgggcggcg aaattcggac
    21181 ggatcggcga gctgcggtgt ggcccggggg cgccagtgtc gacgtctttt cgcgggcggt
    21241 caatagaacg cgtgcatgcg tggggagaga agatggatgc catctatggc acgaccggcg
    21301 cgtcgggatt gcgacggcgc cgccccgttc tcggttcctc tcgacgcaag acgcaacgac
    21361 ggcgccactg cgcgcgttgc cttgccgcgc aaagctgtgg gacacttccc cggtcgcgcg
    21421 caccaatccg acaacaaccg cgggcgccgc gattccatcg cacactggag gattctgcaa
    21481 tgaaggcaat gcaacgagca ttggccgcgg ccgccgcgat cgccgcctcg gttttcgttt
    21541 ccatcgcacc ggcgcaggaa aagtatccat cgcggccggt ggagatcatc gtcccgtggg
    21601 gacccggcgg cggcgccgac cagctcgcgc ggctcaccgc gaagctcacc gagcccgtcc
    21661 tcggcacgtc gtttcccgtg atcaacacgc cgggcgcgac cggcgcgacc ggcatggcga
    21721 agctgctcgc cgagcaggcc gacggctatg cgatggccat ctacattgcc gacacgaatg
    21781 cgctgctcgc cggtccgaat ccacgctgga agctcggcga catcacgccg atcgccgtga
    21841 tgatgcaggt gccgtcgttc ctgttcgtcg ccgagaacag cccgatcaag gattgggcgc
    21901 aattcgagaa ggaggcgcgc gcgaagccga agacgctcaa ggtcgccacg ctcggcttcg
    21961 gcagcgtcga cgacatgacg ctgcagttcc tcgcatccaa gggcatcacc gtcgtcgagg
    22021 tgccgtacgc gaaaccaagc gagcgctacg tctcggtgat cggcggtcac gtcgacgcgc
    22081 tctacgagca ggcgggcgac gtgcgccagt tcatcctgaa caagcagatc cggccgatcg
    22141 tcattttcgg caaggagcgg ctcgcggcgt tcaaggacgt gccgagctcc tacgagctcg
    22201 gatatcacat cgcgctgccg cagttccgct cgtatgtcgt caaggccggc acgccgcccg
    22261 agcgcgtgaa gatactgagc gatgcgatgg ccaaggcggc ggccacaccc gaataccaga
    22321 aattcctcga agagaacttc tcggccaggg acagcttcat cccggccgac aaggccgagc
    22381 cgtatctcgc ggcgcaagtc gcggacatga aggccgcgat ggtgcagaag cagtaagcga
    22441 cggtcgcgtc cacgcgcatg ttcggcaagc tcgcgtcgct cgcgccttat gtggcgctgt
    22501 gcgcggtggc cggctatctg tatcgcgacg ccggcgcatt cgcggcggcc gcgcggcccg
    22561 gccagctcgg tcccgatttc tggccgcgcg cggtcctcgt gctgctgatc gtcgtgtgcg
    22621 tttgcgagat cgtgcgccgc atcgcgttcg agcgtgcggc gcacgacgat tcgtcgtccg
    22681 cgtacgcaag cggcaacgaa gacgcgcagg ccgcttccga agaccgcttt gcctggtgcc
    22741 ttgccggcgg catcgccctc acgatcgcgt acgttctcgc actcgactgg ctcggcttct
    22801 tcgtcgcaac cgcgctctac ctcgcgctgt tcatggtcgt cggccgctat cggcgcacgc
    22861 gcgtgatcgt gtcggccagc gtgcttggca gcctcgcatt cgtctacgtg ttcatgaaga
    22921 tcgtctacgt atcgctgccg ctcggccgag gcccgttcaa ggcactgtcg gtctggctgc
    22981 tcgcgctgct cggcgtgcgc tgaagcggcg cccgcgatgg acgaaacgct gcatcagctc
    23041 gggctcggat tcctgcacct gctgacgctg cccaacgccg cttcgctcgt cgtcggactc
    23101 gtcgtcggca tgctcgtcgc ggtgctgccc ggactcacgc tggtgatggg tgtcgtgctg
    23161 gcgttgccgt tcacctacgg catgagcgtc acgccgtcga tcatcctgct cacggcgatg
    23221 tacctgtcgg ggacctacgg cggcgcgttc acgtcgatcc tgtttcgcat tcccggcgag
    23281 ccgctcgacg tgccgttgtt gtgggacggc tacccgatgg cgcgccaggg caagcccgca
    23341 caggcgctgg gctggacgtt gcttgcggcg ctgtggggcg gcctcatcac ctgcgtgtcg
    23401 gccgtggtgc tcgcggtccc cttcgcgcat ttcgcgctgc gcttcgattc gcccgaatat
    23461 ttcgcggtcg tgctgctcgg tctgtccggc gtcgtcgcgc tcggcggcgc atcgatggcg
    23521 aaggccttga tcagcctgtg catcgggctt ctgcttgcaa cggt
//
LOCUS       DASSVX010000239        20593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1626817, whole genome shotgun sequence.
ACCESSION   DASSVX010000239 DASSVX010000000
VERSION     DASSVX010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 20593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20593
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626817"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1025)
                     /locus_tag="VFO53_10390"
     CDS             complement(168..1025)
                     /locus_tag="VFO53_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008532849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8876725.1"
                     /translation="MTLPSATLLAQSALSGIFIGGLYGLLGLGLTLSWGLLHVINLAH
                     FAFVFLAAYLCYQLASVGGVDPLLTLLLIVPLFAVLGVAVQWLLARFRVSALNSLLAT
                     FGITAIVEAGIQAVWTADFRKLESVYAGLKLRVGGLFIPLPELLTLSLAIALAFGVFA
                     AMRYTRLGRALRALAEDAPVAAAFGINAKAHALILSGASGALAGIAGVCLALSFTLTP
                     SSIYAWVGVVFAAVMLGGLGSALGALAAGLVIGLSEAITMAVASPSWAPIVSFTLLIA
                     LLLVRPGRS"
     gene            complement(1113..2321)
                     /locus_tag="VFO53_10395"
     CDS             complement(1113..2321)
                     /locus_tag="VFO53_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012483921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET8876726.1"
                     /translation="MTIFKRIARRLGVLFAVLLAVSGTALAQAPQPPIRIGSTLALTG
                     PLAATSLIHKVVGEIYVEQLNQRGGLLGRKVEWIVKDDQSKPELARTLYEQLITSDKV
                     DLLMGPYATGAILSAMGVAQRYNKVLVHHTFGIPSMAKYDMQFPAWSLGPEPATTVPN
                     TVFDALAASPAPPKTIAVVTSKFPSVHFMSLGARDVAKKRGLTEVLFLEWDFGNLDYG
                     PIAARIKDAKPDFLWVGAIGLEGNQLLEAMHKIDYTPKEHFYMYPAPGPMSKSPLANN
                     AMSMTVFEEHAPFTSAPGAAEFIRLYHERAAKAGIPDTAVEVQAAASYTAWQILEAGV
                     RGAGSLDDKAIAAYLKKNGATTIQGKLRFDGPSNYGDDLMRVKQVQDGHWVTVYPKAY
                     AAPGATLRVQ"
     gene            complement(2332..3249)
                     /locus_tag="VFO53_10400"
     CDS             complement(2332..3249)
                     /locus_tag="VFO53_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8876727.1"
                     /translation="MHSLDRFVFSVPDIDAAERFYRAFGLDVGRDGNRLELRVYGNAH
                     CWGSIVANGQPKKLQYVCFSAYDDDFDALAARFAHAGATSPHPLSDGRGVWTRDADGT
                     PVQLVAGPKVSASAKGAHAPAAAVVHGKPAAPNRKTAAPVRPRRLSHVLLFTPDVARQ
                     LAFYTGTLGLRLSDRSGDIIAFLHGPHASDHHMIAFAKSHAPGLHHSSWDVASFDDVG
                     LGAERMRAEGYAKGWGVGRHVLGSNYFHYVQDPWGSFAEYSFDIDFVPADVDWPAADH
                     PPEDSFYVWGPAVPDDFVVNHEAPAPAPA"
     gene            3441..4145
                     /locus_tag="VFO53_10405"
     CDS             3441..4145
                     /locus_tag="VFO53_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8876728.1"
                     /translation="MEFGNRTVIVTGASGNLGRAVAGAFGAEGANLVLVARHQQSLAE
                     RFGEEGERRLFVAADLGRQDDANGVAAAARARFGRIDALCNLAGAFRMDGPVHETSDA
                     TWQLLFDANVRSVLCMARACVPAMLAAGGGSIVNVGAHAALRAGANMGAYAASKAAVM
                     RLTEAMAAELRERGINVNCVLPTTIDTPQNRQAMPGADPARWVAPEDLARIIMFLASP
                     AARAIHGASIPVSGLS"
     gene            4177..5274
                     /locus_tag="VFO53_10410"
     CDS             4177..5274
                     /locus_tag="VFO53_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HET8876729.1"
                     /translation="MQRKYSILIMGASYGSLLGAKLLLAGHDVTLVCLPAEAALINSE
                     GFRVRLPVKGSKELVEIDTRRLAGRVSATGTSGVEPGRYDLVALAMQEPQYASPGVRE
                     LLHAVAVARVPCMSIMNMPPLPYLARIPGLDVAALRPCYTDATVWDGFDPALMTLCSP
                     DPQAFRPPDEKVNVLQVSLPTNFKAARFASDAHTAILHRLEAGIEASRLETSAGAVEL
                     PVKLRVHESLFVPLAKWSMLLTGNYRCVQSDGVRSIRDAVHADLAASRAVYEWVASVC
                     RALGASDADLVPFGKYANAAQGLAKPSSAARALAAGARNIERVDRLVQLAAAQKGMRL
                     EAVDRVVALVDGWLERNRNPDREPKRTLATT"
     gene            5343..6482
                     /locus_tag="VFO53_10415"
     CDS             5343..6482
                     /locus_tag="VFO53_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET8876730.1"
                     /translation="MPGLLPNVDPDGLLEYSVVFSDRALNHMSRAFQRVMTHVAQQVE
                     SVYRAPASVIVPGSGTFGMEAVARQFATARRCLVIRNGWFSYRWSQIFDMGAIPSATT
                     VVKARRTAAGREAPFAPAPLGEVVAMIRDQRPELVFAPHVETASGILLPDAYLRGIGE
                     AAHSVGAMFVLDCIASGALWVDMAASGVDILISAPQKGWSSAAGFALVMLGERARKAI
                     DDTTSTSFACDLRKWLSIMDAYKHGTHAYHATMPTDSIARLSDSMQDLEAFGFERAKS
                     AQQELGDKVRRLVAKRGMPSVAAPGFEAPTVVVSYTDDAGIQSGSRFVEAGLQTAAGV
                     PLQCDEGADFRTFRIGLFGLDKLRNVERTVTSLDEALAQLTADVR"
     gene            complement(6489..6929)
                     /locus_tag="VFO53_10420"
     CDS             complement(6489..6929)
                     /locus_tag="VFO53_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET8876731.1"
                     /translation="MANASITGTSYESPATLDVARLLLRLMLGVLILLHGISKIRGGP
                     GFILDIVDKAGLPEPFGYLVYVGEVIAPILVIIGLFTRAAALVIAINMVVAILLVHVS
                     QFFTLADSGGWALELQGLYIVVAVCVALLGAGRYSVGGRHGIWN"
     gene            7168..8475
                     /locus_tag="VFO53_10425"
     CDS             7168..8475
                     /locus_tag="VFO53_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876732.1"
                     /translation="MNDDVIQVNDAASLQDAITQAAVVIEVGGRITGLSTLKLTAGTE
                     LRGGSRAAELHFKAGQPGLMLTASHRISKLRLVVDETQIAIGLSDDTDDLGTLEISDV
                     TTVGRFHLEGNQARKGELRLNNIHVERADARMAAHRPAGFGVEVLLGALTVYNFSKDS
                     TSRWTVDARNLSGGSKEHPLRGSGVFIFGGWFIPDHADMSKAPAPTQEGGSIELVQVT
                     TGEIHADGGIPNGTSNLITGGVFVGSGVHAQRVINEGPVSTYGANDMVLDNWGDVDSW
                     TARATVASHGPSGIGFVNFGNIDTLGIQAAIETYGIGARGFNLYDGSLEKADFQRITT
                     YGDGAIGVQLSKPFGTICVQHDIRTKGGEGDSLVRGKLVHLKAHALSLKEGAKGDEIK
                     VSGQVVAENPAISPYDFGVPSSVIGRISVGGQEIDSTKGLYAR"
     gene            complement(8776..9807)
                     /locus_tag="VFO53_10430"
     CDS             complement(8776..9807)
                     /locus_tag="VFO53_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HET8876733.1"
                     /translation="MDRSTLTRLALLALSLDASGVSAQIASCAAISSDRERLACYDAL
                     AQREPPRDAGAQRAPMPEPGAQDGSAPNAPNAPSALERRWELRPELRHGPFRLLPYRP
                     LYLLVHGTTNTNDAPSSPTRPAPVQDITLQRVEAKLQLSFRTKLAEDMLGSATDVWFG
                     YTQQSYWQAANSRYSSPFRETNYQPEFIAIHPLALEWGGVHLRYAALSFTHESNGRGE
                     SLSRSWNRLIGQLGLESGPWSLQLRPWARVFDATGDRDDNPDIEDYVGRGELVAAYRA
                     NGHVVTFTGRHSLRAGRRSRGSAQLDWAFPIAGALNGHVQLFSGYGESLIDYNHRQTT
                     IGVGVSFFD"
     gene            complement(9789..11174)
                     /locus_tag="VFO53_10435"
     CDS             complement(9789..11174)
                     /locus_tag="VFO53_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET8876734.1"
                     /translation="MNAVARHAACLAAIALSACTLTPPYHRPDAGVPDAYHGPVHEAT
                     ARSIADVGWWNVFGDPRLVALIRDALSGNLDLAIVAAQITQAEAQVSAARSPIFPQVT
                     GQGQAQRSNETPPFATSNSFVAALALSWELDFWGRYRSATEAARARLLATEEARSGII
                     ASLVAGVAQQYLTLNALHQRLDIVNRTAAAQRDSLRLVKLLAEHGVQSATEVRQAESQ
                     VLTTQNQLPAIERQLGATEDALAVLLGKSPRAFDIPATLPADALPPDVPAGLPSELLE
                     RRPDIRQAEQQLIAANANIGVARAQFFPSISLTGSLGRASEALAGLVTSQGITVHALG
                     LAATAPIFEGGALVANERIARAQAEQAAAQYRRTVLVALQEVSDALIAYDRDAAEVKG
                     NRDRVAVSQEYLRLANLRFRAGVISYLEVLDAQRQLFSAQLDQNASELNQRLAIVQLY
                     KALGGGWTEAP"
     gene            complement(11171..14332)
                     /locus_tag="VFO53_10440"
     CDS             complement(11171..14332)
                     /locus_tag="VFO53_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HET8876735.1"
                     /translation="MAQFFIARPVFAIVTALIILLAGGIAGLQLPIAQYPQITLPSIV
                     VSAAYPGASAQTVEQSVAQPIEEQVNGVEGMLYMESTSSSAGTYRLNVTFGLGSNADT
                     AAVQVQNRVAQANARLPSEVTAAGITTSKSTPDTLMYVTLYSPKGSYDSLFLSNYLNI
                     NIVEAIKRIDGVGSVQVFGSDFGMRIWLRPDRMAQLGVTTSDVANAIREQNVQAPAGQ
                     VGQYPSPPHQVFQYGVEVHGRLVTADEFGGIVVKAQPDGSLVHVRDLARVELGAKDYA
                     VQARFDGQPTGTFGVNLTPEASAVAVSKAIRAELEQLKAGFPPDLAYDVVVDKTVFVI
                     ASLDEVVKTLGESLVLVMLVVFLFLQNWRATLIPLLAVPVSLVGTLAAFIALGFTLNT
                     LTLFGMVLAIGIVVDDAIVVVEAVEHHIQEGMDPRAATVQAMREVSGPVVAVALVLAA
                     VFVPVAFLGGIAGALYRQFAVTVAVSTLLSAFVALSLTPALCATLLQPSRGGSGVHAR
                     GPFGVFNRGFERMLRGYSRTVGLAIRRMVIALLLLVALTAGALLLFRQVPSAFVPPED
                     QGFLIGAVVLPEAAALERTMDATARLDGYLAKTPGIVKRLVVNGFNILNGSQQPNGAL
                     FVAALAPWNERTTPATSISGVIGAIFREGAAIPQATVVPLNPPALPGLGAFGGFSFKL
                     EDHRGGTPLELAKVADDFVAAAKKRPEIGSIRSGLNPRTPAYDLRIDREKAKTLGVPI
                     TDIFNALQTFLGGLQVNDFNRFGRSYKVTMQAEPQFRGDISSIKLFHVRNAKGDMVPL
                     STLVTAVSASSPSVLQRYNQFREADISGDPATGYSAGQARAALEAVAAQALPQGYGFE
                     WSGLSLQEQQSSGQTPIVFGLAVLFVFLFLAALYESWSVPFAVLLAVPIGVFGAILAL
                     SVVGITNNIYAQIGLILLIGLSAKNAILIVEFAKVRVDEGMDAVEAAIAAAKLRLRPI
                     LMTSFAFILGVLPLALASGAGAGARVSMGIAVCAGMTFATLFGIFIVPVLFVAIEKLA
                     GRLRRKGPPEDTHRAATPDAKTP"
     gene            complement(14359..15546)
                     /locus_tag="VFO53_10445"
     CDS             complement(14359..15546)
                     /locus_tag="VFO53_10445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET8876736.1"
                     /translation="MHDLLTRWTGIAAIAAIAVAITGCSKPPPASAPAPPAVPVLAMQ
                     VIQRNATVTGELVGQVSAFREVPLRPQTTGFVQKILFEPGQRVHEGELLFVIDPRPYQ
                     AGLSEAQGAVADAAAALARAKGDVARYAPLLPDNAIPRATYDAAVATEKSAEAALDQR
                     KAAAERARLDVRNTEVRSPVTGQIGLQQVEIGGLATAGQTVLATVSTLDPVYVTFSVP
                     EADYVRFMKGAGSPQAAAEQARANKFQLILPDGTVYDQPGTFDFVERAISTTGTLGVR
                     AKFPNPQNLLRPGMNVRVRLVFDEVQNALLVPQRAVTELLGKQFVTTIGTDNKTGQRA
                     VTLGDRVGEMWIVKSGLAAGERIVVDGIQKAPPGTTVAATMITEAQLDNPPTPAPPAA
                     PAK"
     gene            complement(15816..16364)
                     /locus_tag="VFO53_10450"
     CDS             complement(15816..16364)
                     /locus_tag="VFO53_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET8876737.1"
                     /translation="MKNDTPPKTAARDRERDARLGTRSDLPDAATRDIGAALNVLLAD
                     TFALYVKTKNFHWHMSGPHFRDYHLLLDEQADQIYATSDPLAERVRKLGGTTIKSIGH
                     IARLSRVLDNDADYVEPLDMLAELRDDNRSLAARMRETHGLCDELRDVATASLLEVWI
                     DEAERRTWFLFEASRRADPGGR"
     gene            16529..18622
                     /gene="ftsH"
                     /locus_tag="VFO53_10455"
     CDS             16529..18622
                     /gene="ftsH"
                     /locus_tag="VFO53_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET8876738.1"
                     /translation="MKWTEWMAARRADGAAPRGMRRVDRGSGASQAPSPTRIPPRRAW
                     LWFAIALILNFLLARYLVPGAETPLTVPYTLFRDEVVKGNVKAIYSRGETMTGRFAAP
                     VTYPAPEPTGAPAAAPVPAPKARGIPRRGPPRTSAIFETTVPSFAGRDLEQLLLDHNV
                     EISAEPIAEAASPLSTLLFGFGPALLLIAFYVWLFRRTAQQGGGLGGGILGIGKSRAR
                     RYDQESDAKVTFQDVAGIDEAENELIEIVDFLRDPPKYTRLGGTAPKGVLLVGAPGTG
                     KTLLAKAVAGEAGVPFFSMSAAEFVEMIVGVGAARVRDLFKQARENAPAIIFIDELDA
                     IGRARGPTALGGASEQEQTLNQILTEMDGFSSREGIIVLAATNQPDVLDRALLRPGRF
                     DRRVVVNLPDRKGRAAILAVHTRRVPLAPDASLDELAAATPGFSGAELKNLVNEAALL
                     GARRNEQSVGQKDFLDALEKIVLGPARPILLSQADRERIAYHEGGHAILGLVVPGADP
                     VHRVTIVPRGQALGVTYQRPDSDRYNYPEAYLRARIVGMLGGRAAEEIVYGTKTTGAE
                     SDIEQATALARRMVTRWGMSEKIGMVQLAPRDNPYLGGAEGYGASKPFSEQTAEVIDA
                     EVRRIIGECHAEALRLLREHRKALGALAAALLDRETLNEEEILAVTGLERAPTLEGGV
                     APLTGDTPANSLPPD"
     gene            complement(18678..19019)
                     /locus_tag="VFO53_10460"
     CDS             complement(18678..19019)
                     /locus_tag="VFO53_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HET8876739.1"
                     /translation="MKVTASYAMAIALFALAGHAHADARSDYYQRAAQRDTAAFHALD
                     INRDGVVERSEIVGDNDFGPRFRDMDRNDDGVVTEPELALYIREHYGVDAPSANQATM
                     VVQHVATAPSR"
     gene            19095..20267
                     /locus_tag="VFO53_10465"
     CDS             19095..20267
                     /locus_tag="VFO53_10465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8876740.1"
                     /translation="MPGRTLSALIFVLVTCAFATIYITQPVLPILEAQFAIGPSAASL
                     SVSAVIVGMAVTTLPIGVLVDRYPARWLMITGACVIAAAGALCAATTSFAALVAMRAV
                     QGAFIPALTTCVAAWLSRTLPARALNVAMGTYVSATVAGGLGGRLLAGWAFPPAHWRY
                     AFVTIGAALFVVTLLVAPRLAETSPAPSVAPSTPLWRLVVRGPQMLAQVAAFGAFGAF
                     STVFNYFPFYLSRPPWSLSTPTITTLYLVYVAGLVMGPFAGRLANRVGNGVVMIGGAL
                     LLEGALAATLIVAVPALIASLVCLCLGFFAVHAAAVGALNRSVSGERGKANALYTLFY
                     YLGGAAGIAAGGGLYAHAGWPGIVGVSLALSLLPLGVGMIFARRAVTDSSTTQEPS"
     gene            20264..>20593
                     /locus_tag="VFO53_10470"
     CDS             20264..>20593
                     /locus_tag="VFO53_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876741.1"
                     /translation="MKHPLARIASVAFAFLFTGIASAQTAPIVHGDVAAIEGNVLRLT
                     SASGEKTKVRLPDDVRVNLRAPASLDDIKPKTYVGATSTPGPNGTLIASEVHIFPEGR
                     RGAGEGHH"
BASE COUNT         3404 a         6797 c         7110 g         3282 t
ORIGIN      
        1 cgaaaaatag ccggagtagc ccgacaggat gttccacgac gtcgcgagga cgatccaatg
       61 caggacgagg tacaggaacg attcgtagaa cgcgggcaga tgcaggaacg gaatcgtcgc
      121 gagcaccgca cccgcggcga cgatgccggc gagcgtgcgg tcgagtttca ggagcgtccc
      181 gggcgcacca gcagaagcgc gatcagcagc gtgaacgaca cgatcggcgc ccacgatggc
      241 gaggcaaccg ccatcgtgat ggcttccgac agtccgatca cgagaccggc agcaagcgca
      301 cccagcgccg agccgaggcc gccgagcatg accgcggcga agacgacgcc gacccacgca
      361 tagatcgacg acggcgtcag cgtgaacgaa agtgcgaggc agacgccggc gatgcccgcg
      421 agtgcgcccg acgctccgga caggatcagc gcgtgggcct ttgcgttgat gccgaacgcc
      481 gcggcgaccg gcgcgtcttc ggcgagcgcg cgaagcgccc ggccgagccg cgtgtagcgc
      541 atcgccgcaa agacgccgaa tgcgagcgca atcgcgagcg acagcgtgag gagctcgggc
      601 agcgggatga acagcccgcc gacgcgcagc ttcaaaccgg cgtagaccga ctcgagcttg
      661 cgaaagtcgg cggtccagac ggcttgaatg ccggcctcga cgatcgcggt gatgccgaac
      721 gtcgcgagca gcgagttgag cgccgagacg cggaaccgcg cgagcagcca ctgcaccgcc
      781 acgccgagca cggcaaaaag cggcacgatc aaaagcagcg tcaacagcgg atcgacgccg
      841 ccgacgctcg cgagctggta gcaaagatag gcagcaagaa agacgaaggc gaaatgcgcg
      901 agattgatga cgtgcagcag gccccacgac agcgtgaggc cgagcccgag caacccgtac
      961 aggccgccga tgaaaatccc cgacagcgcc gactgcgcga ggagcgtggc actggggagg
     1021 gtcatggatc agccgcccct cacccgcctc gctggggctc ggcgccctct ccccgcaatg
     1081 cggaaagagg gcggggatga ggggtgcatc ggttactgca cgcgaagcgt cgcacccggc
     1141 gccgcgtacg ccttcggata cacggtgacc cagtggccgt cctgcacctg cttgacgcgc
     1201 atcaggtcgt cgccgtagtt gctcggcccg tcgaagcgca gcttgccctg gatcgtggtc
     1261 gcaccgttct tcttgagata cgcggcgatc gccttgtcgt cgagactgcc ggcgccgcgc
     1321 acgcccgctt cgagaatctg ccacgcggtg tacgaggcgg ccgcctggac ctcgaccgcc
     1381 gtgtcgggga tgccggcctt cgccgcacgc tcgtgataga gccggatgaa ctcggccgca
     1441 cccggtgcac tggtgaacgg tgcgtgctcc tcgaacaccg tcatcgacat cgcgttgttc
     1501 gcgagcggcg acttcgacat cggcccgggt gccggataca tgtagaagtg ttccttcggc
     1561 gtgtagtcga tcttgtgcat cgcttcgagg agctgattcc cctcgagccc gatcgcgccg
     1621 acccacagga aatcgggctt cgcgtccttg atgcgcgcgg cgatcggtcc gtagtcgagg
     1681 ttgccgaagt cccattcgag gaacagcacc tcggtcagtc cgcgcttctt cgcgacgtcg
     1741 cgtgcgccga gcgacatgaa gtgcaccgac ggaaacttgc tggtcacgac ggcgatcgtc
     1801 ttcggtggcg cgggcgacgc ggcgagcgcg tcgaacacgg tgttcggcac cgtcgtcgcc
     1861 ggttccggac cgagcgacca cgccggaaac tgcatgtcgt acttcgccat cgatggaatg
     1921 ccgaacgtat gatggacgag caccttgttg tagcgctgcg cgacgcccat cgccgacagg
     1981 atggcacccg ttgcatacgg ccccatcagc agatccacct tgtcggacgt gatcagctgc
     2041 tcgtagagcg tgcgcgcgag ctcgggcttc gactggtcgt ccttcacgat ccattcgacc
     2101 ttgcgtccga gcaagccgcc gcgctggttg agctgctcga cgtagatctc gccgacgacc
     2161 ttgtggatga ggctcgtcgc cgcgagcggt ccggtcagcg ccagcgtgct gccgatgcgg
     2221 atcggcggtt gcggcgcctg cgcgagtgcc gtgccgctca ccgcgagcag caccgcgaac
     2281 aggacgccga gccggcgtgc aatgcgtttg aaaatcgtca tggtttctcc gtcaggccgg
     2341 ggcgggggcg ggtgcttcgt gattgacgac aaaatcgtcg gggaccgcgg gtccccagac
     2401 gtagaacgag tcctcgggcg gatgatcggc cgcgggccag tcgacgtcgg cgggcacgaa
     2461 atcgatgtcg aacgaatact cggcaaagct gccccacgga tcctgcacgt agtggaaata
     2521 gttcgagccc agcacgtggc ggccgacgcc ccagcctttg gcatagcctt ccgcgcgcat
     2581 gcgttcggcg ccaaggccca cgtcgtcgaa gctcgcgacg tcccagctcg aatgatgcag
     2641 ccccggcgca tgcgatttcg cgaaggcgat catgtgatgg tcgctggcat gcgggccgtg
     2701 caggaacgcg atgatgtcgc ccgagcgatc cgataaccgc agcccgagcg tgccggtgta
     2761 gaacgcaagc tggcgcgcga cgtcgggcgt gaagagcagc acgtgcgaca ggcgccgggg
     2821 tcgtacgggc gcggcggttt tccggttcgg tgccgcgggc ttgccgtgca ccacggcggc
     2881 ggccggtgcg tgcgcaccct tggccgacgc cgataccttg ggtccggcga caagctgcac
     2941 cggcgtgccg tcggcatcgc gtgtccatac gccgcgcccg tcggacagcg gatgcggcga
     3001 cgtcgcgccg gcatgcgcga agcgcgccgc gagcgcgtcg aaatcgtcgt cgtacgccga
     3061 aaagcaaacg tactggagct tcttcggctg cccgttcgcg acgatgctgc cccagcaatg
     3121 ggcgtttccg tacacgcgca gttcgagccg gttgccgtcg cggccgacgt cgagcccgaa
     3181 ggcgcggtag aagcgttcgg cggcgtcgat gtcggggacg ctgaaaacga accgatcgag
     3241 cgaatgcacg cccaatgcat tagggcggcg gagcagggca gcttgcgttt ccatggccgg
     3301 aaggctcctg tcgcgcggcg cgcgtcacgg gcggacgata gcaaaaatcc cgtcatggca
     3361 ggaagcggcc gccgcggcgg gcattgccgt gacgccgcgc gcggcgtata cgaggcgctt
     3421 tcgacagcga aggagtcggc gtggaattcg gcaaccggac ggtgatcgtg acgggggcgt
     3481 cgggcaatct cgggcgagcg gtcgccggcg cgttcggcgc cgagggcgcc aatctcgtgc
     3541 tcgttgcgcg gcaccagcaa tcgctggccg aacgcttcgg cgaggagggc gagcggcgtc
     3601 ttttcgtcgc ggccgatctc ggccgtcagg acgacgcgaa tggcgtcgcg gccgccgcgc
     3661 gcgcacgttt cggacgcatc gatgccctct gcaatctcgc cggcgcattt cgcatggacg
     3721 ggccggtgca cgaaacgtcg gacgcgacgt ggcagctgct gttcgacgcg aacgtgcgca
     3781 gcgtgctctg catggcacgc gcgtgcgtgc cggcgatgct cgcggcgggc ggcggcagca
     3841 tcgtcaacgt gggcgcgcac gcggcgttgc gcgccggcgc caacatgggt gcgtatgccg
     3901 cgtcgaaggc cgcggtgatg cgtctcaccg aagcgatggc ggccgagttg cgcgagcgcg
     3961 gcatcaacgt gaattgcgtg ctgccgacca ccatcgacac gccgcagaac cggcaggcga
     4021 tgccgggcgc cgaccccgcg cgctgggtgg cgcccgagga ccttgcgcgc ataatcatgt
     4081 ttcttgcctc accggcggcg cgtgccattc atggcgcgtc gatcccggtc agcggattgt
     4141 cgtgagacaa aaaaagacga taggaggacg atgcaaatgc aacgcaagta cagcatcctg
     4201 atcatgggcg cgtcgtacgg atcgctgctc ggcgcgaagc tgctgctcgc cggtcacgat
     4261 gtgacgctcg tatgcctgcc ggcagaagcc gcgctcatca acagcgaagg tttccgcgtg
     4321 cggctgccgg tcaaaggctc gaaggagctg gtcgagatcg acacgcgccg gctcgcgggt
     4381 cgcgtatcgg cgaccggcac gagcggcgtc gagcctggtc gttacgatct cgtcgcactc
     4441 gcgatgcagg agccgcagta cgcgtcgccc ggcgtgcgcg agctgttgca tgcggtcgcc
     4501 gtcgcaaggg ttccctgcat gtcgatcatg aacatgccgc cgttgccgta cctcgcgcgc
     4561 atcccggggc tcgatgtagc agcacttcgt ccttgctata cggatgcaac ggtgtgggat
     4621 ggcttcgatc cggcgctgat gacgctatgc agtcccgacc cgcaggcgtt ccggccgccc
     4681 gacgaaaagg tcaacgtgct gcaggtcagc ctgccgacga atttcaaggc ggcgcgcttc
     4741 gcatccgatg cgcacacggc gatcctgcat cggctcgagg ccggcatcga ggcaagccgg
     4801 ctcgaaacgt ccgcgggagc ggtcgagctg ccggtcaagc tcagggtgca cgaatcgctg
     4861 ttcgtgccgc tcgccaaatg gagcatgctg ctgacgggca actaccgttg cgtgcagtcc
     4921 gacggcgtgc gctcgatccg cgatgccgtg cacgccgacc tcgcggcgtc gcgcgcggtg
     4981 tacgaatggg tggccagcgt gtgcagggcg ctcggcgcga gcgacgccga tctcgtcccg
     5041 ttcggcaagt atgcgaatgc cgcgcaggga ctcgccaagc cgtcgtcggc ggcgcgcgcg
     5101 ctggccgccg gggcacgcaa catcgagcgc gtcgaccggc tcgtgcaact ggccgcggcg
     5161 cagaagggca tgcggctcga ggcggtcgat cgcgtcgtgg cgctcgtcga cggctggctc
     5221 gagcgcaatc gcaatcccga tcgcgaaccg aagcgaacgc tcgcgacgac ttaaggcgcg
     5281 cggcgcgtgc ctgagcgccc gcgctatcct gtcggtcaag ttccaataag ctggagtcga
     5341 tcgtgcctgg cctccttccg aatgtcgatc ccgatggtct gctcgagtat tcggtcgtgt
     5401 tctccgaccg tgccctcaat cacatgtcgc gcgctttcca gcgcgtgatg acgcacgtcg
     5461 cgcagcaggt cgaatcggtt taccgcgcgc ccgccagcgt gatcgtgccg ggcagcggca
     5521 cgttcggcat ggaagcggtg gcgcgccagt tcgcaacggc caggcgctgc ctcgtgatcc
     5581 gcaacggctg gttcagctat cgctggtcgc agatcttcga catgggcgcc attccctcgg
     5641 caacgacggt cgtcaaggcg cgccgaaccg ccgcggggcg agaggctccg ttcgcgccgg
     5701 cgccgctcgg cgaggtggtg gccatgattc gcgatcaaag gcccgagctg gtgttcgcgc
     5761 cgcacgtcga aacggcatcg ggcattctcc tgcccgacgc gtatctgcgc ggcatcggcg
     5821 aggccgcgca ttcggtcggt gcgatgttcg tcctcgattg cattgcatcg ggtgcgctgt
     5881 gggtcgacat ggcggcgagc ggcgtcgaca tcctgatcag cgcaccgcag aaaggctgga
     5941 gcagcgcggc aggattcgcg ctggtcatgc tcggcgagcg cgccaggaag gcgatcgacg
     6001 acaccacgag cacgagcttc gcatgcgatc tgcgcaagtg gctgtcgatc atggatgcgt
     6061 acaagcacgg cacgcatgcg taccacgcga cgatgccgac cgattcgatc gcgcgcctgt
     6121 ccgattcgat gcaggacctc gaagccttcg gcttcgagcg cgcgaagagc gcgcagcagg
     6181 agctcggcga caaggtgcgc cgcctcgtcg cgaagcgcgg gatgccgagc gtcgccgcgc
     6241 cgggtttcga ggcgccgacc gtcgtcgtca gctacaccga cgatgcgggc atccagtcgg
     6301 gaagccggtt cgtcgaggcc ggcctgcaga ccgccgccgg cgtgccgttg caatgcgacg
     6361 aaggagcgga tttcaggact ttccgcatcg gcctcttcgg cctcgacaag ctgcgcaacg
     6421 tcgagcgcac ggtgacgagt ctcgacgagg cgctggcgca gctcaccgcc gacgtgcgct
     6481 gactccgctc agttccatat gccgtgtcgg ccgccgacac tgtagcggcc ggcgccgaga
     6541 agggccacac acacagcgac gacgatatac agtccctgca gctccagcgc ccagccaccg
     6601 gaatcggcaa gcgtgaagaa ctgcgaaacg tgcacgagca ggatcgcgac aaccatgttg
     6661 atcgcgatca ccagcgcggc ggcgcgcgtg aaaagtccga tgatcacgag gatcggggcg
     6721 atgacctcgc ccacgtacac gagatagccg aagggttccg gcagcccggc cttgtccacg
     6781 atgtcgagga tgaagcccgg gccgccccgg atcttgctga tcccgtgcag caggatcagc
     6841 acgccgagca tcagccgcag caggagccgg gcgacgtcga gcgtggcggg ggattcgtaa
     6901 ctggtaccgg tgatcgaagc gttcgccatg ctgtcctcct tcagtgatgg atgcggggaa
     6961 acgcgatgcc tcggaacctg caactcgcac gccgcgtgca atcgtcggca gcatagtatt
     7021 ctgaattctg cgagattcgg tagcccaacc gcgcattccg ttcaacaccg ggcaacgacg
     7081 cgttgcccgg cgttccagca ggaacaaacc ggtcgcgcta ccatcgaacg catctgctct
     7141 cgccgtcaac agcatgagga gattgccgtg aacgatgacg ttatccaggt caacgatgcc
     7201 gcttctcttc aggatgcaat cacgcaagcg gccgtggtca tcgaagtggg cggccggata
     7261 accggccttt ctacgttgaa actgaccgcg ggtaccgaac tgcgtggcgg aagtcgagcc
     7321 gcggaactgc atttcaaagc cggccagccg gggctgatgc tgactgcaag ccatcgaatc
     7381 tccaagctgc gactggtcgt cgatgagacc cagatcgcaa tcggcctttc cgacgatacc
     7441 gatgacctcg gcaccctcga gatcagcgac gtaacgacgg tgggccgatt ccatctggaa
     7501 ggcaaccagg caaggaaagg cgagctgaga ctgaacaaca ttcatgtcga gcgcgccgac
     7561 gcgcggatgg cggcgcaccg tccggcgggc ttcggcgtgg aagtgctgct cggcgcactg
     7621 acggtctaca acttctcgaa ggacagcacg agtcgctgga ccgtcgacgc ccgtaacctt
     7681 tcgggcggca gcaaggagca tccgctacga ggcagcggcg tgttcatctt cggcggctgg
     7741 ttcattccgg atcacgccga catgagcaaa gcccccgcgc ccacgcagga aggcggcagc
     7801 atcgagctcg tgcaggtgac taccggcgaa attcatgcgg acggcggcat cccgaacggc
     7861 acgtccaacc tcatcacggg aggcgttttt gtcggctcgg gcgtgcacgc gcagcgcgtc
     7921 atcaacgagg gccccgtcag cacctacggc gccaatgaca tggtgctgga taactggggc
     7981 gatgtcgact cgtggacagc acgcgcgacg gtcgcatcgc atggaccgag cggcatcggt
     8041 ttcgtgaact tcgggaacat cgacacgctt ggtattcaag ccgccatcga aacctacggc
     8101 ataggcgcgc gtggcttcaa cctgtacgac ggctcgcttg aaaaagccga ctttcagagg
     8161 atcacgacct acggcgacgg cgccatcggc gtacaacttt ccaagccctt tggcacgatt
     8221 tgcgttcagc acgacatccg caccaagggt ggcgaaggag acagcctggt gcgtggcaag
     8281 ctcgtccatc tgaaggccca tgcactgagc ctgaaggaag gcgcgaaggg tgacgagatc
     8341 aaagtgagcg gtcaggtcgt cgccgagaat cccgcgatct ctccctacga tttcggcgtg
     8401 ccgtcgagcg tcattgggag gatatcggtc ggcgggcagg agatcgattc gaccaagggc
     8461 ctgtatgcgc ggtagatagg ggagacacgt tacgccagcg ggcggagccg cgtgctgtgc
     8521 gcgactcgcg aacgcgtcgc acccgctccg cgctttcgac ctcggtaaac gcaacggacg
     8581 cgaaatcgcg gggcgtccgc catcaacccg ttcagcggcc gcgtggcagc acggtgtaga
     8641 cccgtgcgat gaaagcgcgg aaatccgcca tgtagccgcg caggaagtct tcgtttggaa
     8701 gcgggcagcg acatgccagc tcgaaatccg cgaagcgcgg gtcctgttag cgagcgcgtt
     8761 tcgccgctcg tgcaatcagt cgaagaacga tacgccgacg ccgatcgtcg tctgccggtg
     8821 gttgtagtcg atcaggcttt cgccgtagcc ggagaagagc tgcacatgac cgttcagcgc
     8881 gccggcgatc ggaaacgccc agtcgagttg cgccgagccg cgtgagcgtc ggcccgcccg
     8941 aagcgaatgc cggccggtga acgtcacgac gtgtccgttc gcccgatacg ccgcgacgag
     9001 ctccccgcgg ccgacgtagt cctcgatgtc gggattgtca tcgcgatcgc cggttgcgtc
     9061 gaacacgcgc gcccacggcc gcaattgcag cgaccacggg cccgattcga ggccaagctg
     9121 cccgatgagc cggttccagc tccgcgacag cgattcgcca cgcccattcg attcgtgcgt
     9181 gaagctcaac gccgcgtagc gcagatgaac cccgccccat tcgagcgcga gcggatggat
     9241 cgcaatgaat tccggctggt agttcgtctc gcgaaacggc gacgaatagc ggctgttcgc
     9301 cgcctgccag tacgattgct gcgtgtagcc gaaccagaca tcggtcgcgc tgccgagcat
     9361 gtcctcggcg agcttggtgc gaaagctgag ttgcagcttc gcttccaccc gctggagcgt
     9421 gatgtcctgc accggtgccg ggcgcgtcgg cgagctcggc gcgtcgttcg tgttcgtggt
     9481 gccgtgcacg agcaggtaca gcggccggta cgggagcaac cggaacggcc catgccgcag
     9541 ctcgggacgc aattcccaac gccgctcgag cgcactcggc gcattcggcg cattcggcgc
     9601 agatccatct tgcgcgccgg gctccggcat cggcgcgcgt tgtgcgccgg cgtcgcgggg
     9661 cggctcgcgc tgcgcgagcg cgtcatagca ggcaagccgt tcgcgatcgc tgctgatcgc
     9721 ggcgcacgat gcgatctgcg cgctcacgcc ggatgcatcg agcgagagcg ccaatagcgc
     9781 gagccgcgtc agggtgcttc tgtccatccg cctcccagtg ccttgtagag ctgcacgatg
     9841 gccagccgct ggttgagctc gctcgcattc tggtcgagct gcgccgagaa aagctggcgc
     9901 tgcgcatcga gcacctcgag atagctgatc acgccggcac gaaagcgaag gttggcaagc
     9961 cgcagatatt cctgcgacac ggccacccga tcgcgattgc ccttcacctc ggcggcgtcg
    10021 cgatcgtaag caatcagcgc atcggagact tcctgcagcg ccaccagcac cgtgcgccga
    10081 tattgcgccg cggcctgctc ggcctgcgcc cgcgcgatcc gttcgttcgc gacgagcgcg
    10141 ccaccctcga agatcggcgc ggtcgcggcg aggccgagcg catgcacggt aatcccctgc
    10201 gatgtgacga ggccggcgag cgcttcgctc gcccggccga gcgatcccgt gagcgagatc
    10261 gacgggaaga actgcgcgcg ggcgaccccg atgttggcgt tcgccgcgat cagctgctgc
    10321 tcggcctgcc ggatgtcggg ccgccgctcg agcaactcgg acggaagccc ggcggggacg
    10381 tccggcggca gcgcgtccgc aggcagcgtt gcagggatgt cgaaagcgcg gggcgacttg
    10441 ccgagcagga ccgccagcgc atcctcggtt gcgccgagct gccgctcgat cgcgggcagc
    10501 tgattttgcg tggtgagcac ctgcgactcg gcctgccgca cctcggtggc cgattgcacg
    10561 ccgtgctcgg cgaggagctt cacgagacgc agcgaatcgc gctgcgccgc ggcggtgcgg
    10621 ttcacgatgt cgaggcgctg gtgcagcgca ttgagcgtga ggtactgttg cgcgacgccg
    10681 gcgacgagcg acgcaatgat gccgctgcgc gcctcctcgg tggcgaggag gcgcgcacgc
    10741 gccgcctccg tggcgcttcg ataacgtccc cagaagtcga gctcccacga caacgcgagt
    10801 gcggcgacga agctgttcga agtcgcgaac ggcggcgtct cgttgctgcg ctgtgcctgc
    10861 ccctggcccg tgacctgcgg gaagatcggc gagcgtgccg cgctcacctg cgcctcggcc
    10921 tgggtgatct gcgcggcgac gatcgcgagg tcgagattgc cggacagcgc gtcgcgaatg
    10981 agtgccacga gtcgcggatc gccaaagacg ttccaccacc cgacgtcggc aatcgagcgc
    11041 gccgtcgctt cgtgcaccgg cccgtggtac gcatccggca cgcccgcatc gggacgatga
    11101 tagggcggcg tgagcgtgca ggccgacagc gcgatggcgg cgaggcacgc tgcgtggcgc
    11161 gcaacggcgt tcatggcgtc ttcgcatcgg gtgtcgcggc ccggtgcgta tcctcgggcg
    11221 gccctttgcg ccgcaggcgg ccggcgagct tctcgatggc gacgaacagc accggcacga
    11281 tgaagatgcc gaacagcgtg gcgaatgtca tgcccgcaca cacggcgatg cccatcgata
    11341 cgcgcgcgcc ggcgccggca ccgctggcga gggcaagtgg cagcacgccg agaatgaacg
    11401 cgaacgacgt catcaggatc ggccgcagcc gcagcttcgc cgccgcgatc gcggcttcga
    11461 cggcgtccat tccttcgtcg acgcgcacct tcgcgaattc gacgatcagg atcgcgttct
    11521 ttgccgacag gccgatcagc aggatgaggc cgatctgcgc atagatgttg ttcgtgatgc
    11581 cgacgacgga gagcgcgagg atcgcgccga acaccccgat cggcacggca agcagaacgg
    11641 cgaacgggac cgaccagctc tcgtagaggg cggcgaggaa caggaaaacg aagaggaccg
    11701 cgagcccgaa cacgatcggt gtctggccgg acgattgctg ctcctgcaac gacagccccg
    11761 accattcgaa gccgtaaccc tgcggcagcg cctgcgccgc gaccgcctcg agcgcggcac
    11821 gcgcctgtcc cgcgctgtag cctgtcgccg ggtcacccga gatgtcggcc tcgcgaaact
    11881 ggttgtagcg ctgcagcacc gacggtgagc tcgcgctcac cgccgtgacg agcgtcgata
    11941 gcggcaccat gtcgcccttg gcgttgcgca cgtggaagag cttgatcgac gagatgtcgc
    12001 cgcggaactg cggctcggcc tgcatcgtca ccttgtagct gcggccgaag cggttgaagt
    12061 cgttcacctg cagtccgccc aggaacgtct gcagcgcgtt gaagatgtcg gtgatcggca
    12121 cgccgagcgt cttcgccttt tcccggtcga tgcgcaggtc gtacgccggc gtgcgcgggt
    12181 tgaggcccga gcggatcgag ccgatttcgg gccgcttctt cgctgccgcg acgaaatcgt
    12241 cggcgacctt cgcaagctcg agcggcgtgc cgccgcgatg atcctcgagc ttgaagctga
    12301 agccgccgaa cgcgccgagc cccggcagtg ccggcggatt gagcggaacg accgttgcct
    12361 gcgggatcgc cgcgccttcg cggaagatcg cgccgatgac gccgctgatg ctcgtcgccg
    12421 gcgtggtgcg ctcgttccat ggtgccagcg ccgcgacgaa cagcgcgcca ttgggttgtt
    12481 gcgagccgtt gaggatgttg aagccgttga cgacgagccg cttgacgata cccggcgtct
    12541 tcgcaaggta accgtcgagc ctggcggtcg cgtccatcgt gcgctcgagc gccgccgctt
    12601 cgggcagcac gacggcgccg atcaggaacc cctggtcctc cggcggcacg aatgcgctcg
    12661 gcacttgccg gaacagcaac agtgcgccgg cagtgagggc gacgaggagc agcagcgcaa
    12721 tgaccatgcg ccggatcgcg agaccgacgg tgcggctgta gccgcgcagc atccgctcga
    12781 agccgcggtt gaacacgccg aacggtccgc gcgcgtgcac gccgcttccg ccgcgcgagg
    12841 gctgcaacaa cgtcgcgcac agcgccgggg tgagcgacag cgcaacgaac gccgacagca
    12901 gcgtcgatac cgcgaccgtc acggcgaact gacggtagag cgcgcccgcg atgccaccga
    12961 ggaaggcaac cggaacgaac acggcggcga gcacgagcgc cacggcgacg accggcccgg
    13021 aaacctcgcg catcgcctgc acggtggccg cgcgcgggtc catgccttcc tggatgtggt
    13081 gctctaccgc ctcgacgacg acgatcgcgt cgtcgacgac gatgccgatc gcgagcacca
    13141 tgccgaacag cgtcagcgtg ttgagcgtga agccgagcgc gatgaacgcg gccagcgtcc
    13201 cgacgagcga taccggcacc gctagcaagg gaatgagcgt cgcgcgccag ttctgcagga
    13261 agaggaacac gacgagcatg acgagcacga gcgattcacc gagcgtcttg acgacttcgt
    13321 cgagcgacgc gatgacgaat accgtcttgt cgacgacgac gtcgtacgcg agatccggcg
    13381 gaaagccggc cttcagctgc tcgagctcgg cgcggatcgc cttcgacacg gcgaccgcgc
    13441 tcgcttccgg cgtcaggttc acgccgaacg tgccggtcgg ctggccgtcg aagcgcgcct
    13501 gcaccgcgta atccttcgcg ccgagctcga cgcgcgcgag gtcgcgcacg tgcacgagcg
    13561 agccatcggg ctgcgccttg acgacgatgc cgccgaactc gtcggcggtg acgagccgtc
    13621 cgtgcacctc gacgccgtac tggaacacct ggtgcggcgg cgacgggtac tgcccgacct
    13681 ggcccgcggg cgcctgcacg ttctgctcgc ggatcgcatt cgcgacgtcg gacgtcgtta
    13741 cgccgagctg cgccatccga tcgggccgca gccagatgcg catgccgaag tcgctgccga
    13801 acacctgcac gctgccgacg ccgtcgatgc gcttgatcgc ctcgacgata ttgatgttga
    13861 gatagttgct gaggaacagc gagtcgtagc ttcccttcgg cgaatagagc gtgacgtaca
    13921 tgagcgtgtc gggcgtgctc ttgctcgtcg tgatgccggc cgccgtcacc tcggacggca
    13981 ggcgcgcgtt cgcctgcgcg acgcggttct gcacctggac ggcggcggtg tcggcgttgc
    14041 tgccgagacc gaacgtcacg ttgagccggt acgtgcccgc cgatgacgac gtcgactcca
    14101 tgtagagcat gccctcgacg ccattgacct gttcctcgat cggctgcgca accgattgct
    14161 cgacggtttg cgcactggcg cccgggtagg ccgccgacac cacgatgctc ggcagcgtga
    14221 tctgcggata ctgcgcgatg ggcagctgca gtcctgcgat gccgcccgca agcaggatga
    14281 tgagcgcggt gacgatcgcg aagaccggcc gcgcgatgaa gaactgcgcc atctacgaaa
    14341 gccgcgctgc cggcggcgtc atttcgccgg tgccgccggc ggcgctggcg tgggaggatt
    14401 gtcgagctgc gcttcggtga tcatcgtcgc cgcgaccgtg gtgccggggg gcgccttctg
    14461 gatgccgtcg acgacgatgc gctcgccggc ggcaaggccc gacttcacga tccacatctc
    14521 gccgacacgg tcgccgagcg tgacggcgcg ctgcccggtt ttattgtccg tgccgatcgt
    14581 cgtgacgaac tgcttgccga ggagctcggt gaccgcccgc tgcggcacga gcagcgcgtt
    14641 ttgcacctca tcgaacacga ggcgcacgcg cacgttcatc cccggcctca gcagattctg
    14701 cggattcggg aatttcgcgc gcacgccgag cgtgccggtg gtgctgatcg cccgctcgac
    14761 gaagtcgaac gtgccgggct ggtcgtacac ggtgccgtcg ggaaggatca gctggaactt
    14821 gttggcgcgc gcctgctcgg cggccgcctg cggcgaaccg gcgcccttca tgaagcgcac
    14881 gtaatcggct tccggaacgc tgaacgtcac gtagacgggg tcgagcgtgg acaccgtcgc
    14941 gaggaccgtc tggccggccg tcgcaagccc gccgatttcc acctgctgca ggccgatctg
    15001 accggtgacc ggcgaacgca cttcggtgtt gcggacgtcg aggcgcgcgc gctcggcggc
    15061 ggccttgcgc tggtcgagcg ccgcctcggc ggacttttcg gtggcgaccg ccgcgtcgta
    15121 ggttgcccgg ggaatggcgt tgtcgggcaa cagcggcgca tagcgcgcga catcgccctt
    15181 cgcgcgggca agcgctgccg cggcgtcggc caccgcaccc tgcgcttcgg agagccctgc
    15241 ctggtagggg cgcggatcga tgacgaacag aagctcgccc tcgtgcacgc gctgccccgg
    15301 ctcgaacagg atcttctgga cgaaccccgt ggtttgcggg cgcagcggca cttcgcgaaa
    15361 ggcgctcacc tgaccgacca gctcgccggt gaccgtcgcg ttgcgctgaa tgacctgcat
    15421 ggccagcacg ggaacggccg gcggtgccgg cgccgatgcc ggcggcggct tcgagcagcc
    15481 ggtgatcgcg acggcgatgg cggcgattgc ggcgatgccg gtccatcgcg tgagcaggtc
    15541 gtgcatgccg cgtatcctgt ttttacaatc gtggagtcgg ccgcgacgcc agaggcgctg
    15601 caacaccccg cgcaatgcac cggcggagcg caacgattcg agtgacgtgg cgcgacttcg
    15661 ttccggcgcc gccggaacag gatcaccctg gcgcgaactc tgctctactg gattcgatgc
    15721 cgtccatgca aacgaccgct cgcgcgcgca acgacggcga tgcccgccat gccggtctcg
    15781 ggacgtggca cgcgcggcga atgccgcgcg tcgcgtcagc gcccgcccgg atcggcgcgc
    15841 cggctcgcct cgaacaggaa ccaggtgcgc cgctcggcct cgtcgatcca tacctcgagg
    15901 aggcttgcgg tggccacgtc gcgaagctcg tcgcaaagac cgtgcgtttc gcgcatgcgc
    15961 gcggcaagcg agcgattgtc gtcgcgcagc tcggcgagca tgtcgagcgg ctcgacgtag
    16021 tcggcatcgt tgtcgagcac gcgggagagc cgcgcgatgt ggccgatcga tttgatcgtc
    16081 gtgccgccga gcttgcgcac gcgctcggcg agcgggtcgc tggtcgcgta gatctggtcg
    16141 gcctgctcgt cgagcagcaa atggtaatcg cggaagtgcg gcccgctcat gtgccagtgg
    16201 aaattcttcg tcttgacgta gagcgcgaac gtatcggcga gcagcacatt cagcgcagcg
    16261 ccgatatcgc gggtcgcggc gtccggaagg tcgctgcgcg tgccgagcct cgcgtctcgt
    16321 tcgcggtcgc gggcagcggt cttcggcggt gtgtcgttct tcatggggat cctttccagg
    16381 tcgtcgctat gcatgggcaa gaggcgcctt ccttcgttcc gtcgacgcat gcatcggttg
    16441 caattgtgcg ccgtgaaacg ccgccgcatt gcgccggtct gcaccatgag gcccgccggg
    16501 ggcggccacg cgcgaggagg cacctagcgt gaagtggaca gaatggatgg cggcacggcg
    16561 ggccgacggc gccgcgccgc gcgggatgcg ccgcgtcgac cgcgggtcgg gcgcgtcgca
    16621 ggcgccgtcg ccgacgcgaa taccgccgcg ccgcgcatgg ctgtggttcg cgattgcgct
    16681 gatcctcaat ttcctgctcg cgcgctatct cgtgccaggt gccgagacac cgctgaccgt
    16741 gccgtacacg ctgtttcgcg acgaggtcgt gaagggcaat gtcaaggcga tctacagccg
    16801 tggcgagacg atgacgggcc gcttcgcggc gccggtcacc tatccggcgc ccgaaccgac
    16861 gggcgcgccg gcggcggcac ccgtccctgc gccgaaggcg cgcgggatcc cgcgacgcgg
    16921 gccgccgcga acctccgcga tcttcgagac caccgtgccg tcgttcgccg gccgcgacct
    16981 cgagcaactg ctgctcgatc acaacgtcga gatcagcgcc gagccgatcg ccgaagcggc
    17041 cagtccgttg tcgacgctgc ttttcggctt cggtcctgcg ctgctgttga tcgctttcta
    17101 cgtctggctg ttccggcgta cggcgcagca gggcggcggg ctcggcggcg gcatcctcgg
    17161 catcggcaag agccgcgcgc gacgctacga ccaggagagc gacgcgaagg tcacgttcca
    17221 ggatgtcgct ggcatcgacg aagcggaaaa cgagctgatc gagatcgtcg atttcctgcg
    17281 cgatccgccg aagtacacgc ggcttggcgg caccgcgccg aagggcgtgc tgctcgtcgg
    17341 tgccccgggc accgggaaga cgctgctggc caaggcggtg gcgggcgagg cgggcgtgcc
    17401 gttcttctcg atgagcgcgg cggagttcgt cgagatgatc gtcggcgtcg gtgcggcgcg
    17461 tgtccgcgat ctgttcaagc aggcacgcga gaatgcgccg gcgatcatct tcatcgacga
    17521 gctcgacgcg atcggccgcg cgcgcggtcc cacggcgctc ggcggcgcga gcgagcagga
    17581 gcagacgctg aaccagatcc tgaccgagat ggacggcttc tcgagccgcg agggcatcat
    17641 cgtgctggcg gcgacgaacc agcccgacgt gctcgaccgg gcgctgctgc ggcccgggcg
    17701 cttcgaccgg cgcgtcgtcg tgaacctgcc cgaccgcaag ggacgcgcgg cgatccttgc
    17761 ggtgcatacg cggcgggtgc cgctcgcgcc cgacgcgagc ctcgacgagc ttgccgcggc
    17821 cacgcccggg ttctcgggcg cggagctcaa gaacctcgtc aacgaggcgg cactgctcgg
    17881 cgcgcggcgc aatgagcaga gtgtcgggca gaaggatttt ctcgacgcgc tggagaagat
    17941 cgtcctcggg ccggcgcgac cgatcctgct gtcgcaggcc gaccgcgagc gcatcgcgta
    18001 tcatgaaggc ggtcacgcga tcctcggcct cgtggtaccg ggcgccgatc cggtgcaccg
    18061 cgtgacgatc gtgccgcgcg gacaggcgct cggcgtcacg taccagcgac ccgacagcga
    18121 ccgctacaac tatcccgagg cgtacctgcg cgcgcgcatc gtcggcatgc tgggcggccg
    18181 tgccgccgag gagatcgtct atggaacgaa gacgaccggc gccgaaagcg acatcgagca
    18241 ggccaccgcg ctcgcacgcc ggatggtcac gcgctggggg atgagcgaga aaatcggcat
    18301 ggtccagctc gcgccgcgcg acaatccgta cctcggcggt gccgagggtt acggcgcgtc
    18361 gaagcccttc agcgagcaga ccgcggaggt gatcgacgcc gaggtgcgcc ggatcatcgg
    18421 cgaatgccac gccgaggcgt tgcggctgct gcgcgagcat cgcaaggcgc tcggcgcgct
    18481 cgccgcggcg ctgctcgacc gcgagacgct gaacgaggag gagattctcg ccgtcaccgg
    18541 cctcgagcgc gcaccgacgc tcgagggcgg cgttgcgccg ctgaccggcg atacgccggc
    18601 caattcgctg ccacccgatt aggccgctga ccggcgatac gccggccaat tcgctgccac
    18661 ccgattactt ttcgtgctta ccgcgacggt gccgtggcga cgtgctgcac gaccatcgtt
    18721 gcctggttcg cggacggcgc gtccacgcca tagtgctcgc ggatgtaaag ggccagctcc
    18781 ggctcggtga cgacgccgtc gtcgttgcga tccatgtcgc gaaagcgcgg cccgaagtcg
    18841 ttgtcgccga cgatctcgct gcgctcgacc acgccgtcgc ggttgatgtc gagcgcgtgg
    18901 aacgccgccg tgtcgcgttg cgcggcgcgc tggtaatagt cgctgcgggc gtcggcgtgc
    18961 gcatgcccgg cgagggcgaa gagcgcgatt gccatcgcgt aagatgccgt gaccttcatc
    19021 gtcgtctccg tttcatctct taaacgagac atcaaatcgc cgcccgggtt caaggcaacg
    19081 ccgccgcgca gaacgtgccc ggccgtacgc tttcggcgct tatcttcgtg ctggtgacgt
    19141 gcgcgttcgc caccatctat atcacgcagc ccgtgctgcc gatcctggag gcgcaattcg
    19201 cgatcgggcc gtcggcggcg tcgctgtcgg tttcagccgt gatcgtcggc atggcggtca
    19261 cgacgctgcc gatcggcgtg ctggtcgacc gctatccggc gcggtggctg atgatcactg
    19321 gcgcttgcgt catcgcggcg gccggtgcgc tatgcgcggc gacgacgagc ttcgcagcac
    19381 tcgttgcgat gcgcgcggtg cagggcgcgt tcattccggc gctcaccaca tgcgttgccg
    19441 catggctttc gcgcacgctg ccggcgcgcg cgctcaatgt cgcgatgggc acgtacgtct
    19501 cggcaacggt ggccggcggg ctcggcgggc gcctcctcgc cggctgggcg ttcccgccgg
    19561 cgcattggcg ttatgcgttc gtaacgattg gcgccgcgct gttcgtcgtc acgctgctcg
    19621 ttgcgccgcg acttgccgaa acgtcgccgg cgccctccgt cgccccttcg acgccgcttt
    19681 ggcgcctcgt cgttcgcggc ccgcagatgc tcgcgcaggt cgcggcgttc ggcgcgttcg
    19741 gcgccttctc gacagtcttc aactatttcc cgttctacct gtcgcggcct ccgtggtcgt
    19801 tgtcgacgcc gacgatcacg acgctgtacc tggtctacgt cgccggcctc gtcatgggac
    19861 ctttcgccgg acggctcgcg aaccgcgtcg ggaatggcgt ggtgatgatc ggcggcgcgc
    19921 tgctgctcga aggcgcgctt gcggcgacgc tgatcgtcgc ggtgccggcg ctcatcgcaa
    19981 gcctcgtctg cctttgcctc ggcttcttcg cggtccatgc cgcggccgtc ggcgcgctga
    20041 accgcagcgt ctccggtgaa cgcggcaagg ccaacgcgct ctatacgttg ttctactatc
    20101 tcggcggcgc cgccgggatc gccgcgggcg gcggcctgta cgcgcatgcc ggatggccgg
    20161 gaatcgtcgg cgtgtcgctc gcgctgtcgc ttttgccgct cggcgtcggg atgatcttcg
    20221 cgcgccgcgc tgttaccgat tcgtcgacga cgcaggaacc ctcatgaagc atccgcttgc
    20281 ccgcatcgcc tctgtcgcgt tcgcgtttct cttcacgggc atcgcgtccg cgcaaacggc
    20341 gccgatcgtc cacggcgacg tcgcggcgat cgaaggcaac gtgctgcgcc ttacgtcagc
    20401 gtccggcgaa aagacgaaag tccgcctgcc cgacgacgtg cgggtgaacc tgcgcgcgcc
    20461 cgcgtcgctc gacgacatca agccgaaaac gtacgtcggc gccacgtcga cgcccggacc
    20521 gaacggcacg ctgatcgcga gcgaagtgca catctttccg gaagggaggc gcggtgctgg
    20581 cgaggggcac cat
//
LOCUS       DASSVX010000240         8746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_726951, whole genome shotgun sequence.
ACCESSION   DASSVX010000240 DASSVX010000000
VERSION     DASSVX010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726951"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFO53_10475"
     CDS             complement(<1..507)
                     /locus_tag="VFO53_10475"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET8876742.1"
                     /translation="MSWLTKLLPPRIKSTPGAARKTPVPEGLWVKCASCEATLYRTDL
                     EKNLLVCPKCNYHNRIPARERIEQLLDAEGRFEIGLEVVPVDSLKFKDTKRYPERLEA
                     ALEETGETDALVVMQGSVKSVPLVLAVFEFNFMGGSMGSVVGERFVRGVRVAYENRLP
                     FVCVSASGG"
     gene            complement(619..1464)
                     /gene="trpA"
                     /locus_tag="VFO53_10480"
     CDS             complement(619..1464)
                     /gene="trpA"
                     /locus_tag="VFO53_10480"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET8876743.1"
                     /translation="MSGQHPSAHLNRIDRVFERQRIAGRTVLIPYVTAGDPSLATTAA
                     ILDTLAEAGADVIELGVPFSDPMADGPVIQRASERALAQGVALTGVLDLVAQFRKRNA
                     HTPIVLMGYANPIEAMGVTVFVDRARDAGVDGVIVVDYPPEEATEFAALLRARDVAPI
                     FLIAPTTPQSRIERIARLAAGYVYYVSLKGVTGAGHLDTGDVARKLDEIRRHITLPVG
                     VGFGIRDADSARAIAAHAEAVVIGSRLIVEIEEAAPGEAAMRAGAFVATIRRALDTRL
                     AACVA"
     gene            complement(1461..2669)
                     /gene="trpB"
                     /locus_tag="VFO53_10485"
     CDS             complement(1461..2669)
                     /gene="trpB"
                     /locus_tag="VFO53_10485"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET8876744.1"
                     /translation="MAELSRVRDLPDERGHFGPYGGVFVAETLIGALDELTVQYARYR
                     DDPQFVAEFEHELEHYVGRPSPVYHAERWSRELGGAQIFLKREDLNHTGAHKINNCIG
                     QALLARRLGKRRVIAETGAGQHGVATATVAARFGMECVVYMGSEDVRRQAQNVYRMNL
                     LGATVVPVESGSKTLKDALNEAMRDWVTNVDTTFYIIGTVAGPHPYPMLVRDFQSVIG
                     RECLTQMPEAAGRDPDVVIACVGGGSNAMGIFHAYVPNPAVRLVGVEAAGEGIATGRH
                     AASLVAGRPGVLHGNRTYLLQDANGQIIETHSISAGLDYPGVGPEHAWLKDTARAEYV
                     SITDDEALAAFHACCRIEGIIPALESAHALAYATKLAPTLPRDRILLVNLSGRGDKDM
                     ATVAERSAVR"
     gene            complement(2644..3426)
                     /locus_tag="VFO53_10490"
     CDS             complement(2644..3426)
                     /locus_tag="VFO53_10490"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET8876745.1"
                     /translation="MRAWHSPQRGATFPRNSHEAVFTLLAGHVTPNAERPRTRVKICG
                     ITRAGDARAAADAGADAIGLVFWPGTPRVVTHAQARSIVAALPPYVSVVGLFVDPEAQ
                     AVREALAAVPIDVLQFHGSEPAPFCRAFGRRYVKAIAVRDDVDLLESLSPYDDAAGLL
                     FDAFRAGDLPGGTGRVFDWRRLSADWRARLRAPLILSGGLDADNVERAIRAVAPWAVD
                     VSSGVEERASDGTPRRGVKDATRIRAFVQGVRSADGGAFPST"
     gene            complement(3371..4156)
                     /gene="truA"
                     /locus_tag="VFO53_10495"
     CDS             complement(3371..4156)
                     /gene="truA"
                     /locus_tag="VFO53_10495"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET8876746.1"
                     /translation="MRRALALEYDGSGYCGFQSQSSGCGIQDALERALAAIAGVPVRA
                     IPAGRTDAGVHATSQIVHFDVPVERPSTAWVRGVNAHLPPSVAVLWCAGVPSDFHARF
                     GAIARHYTYVLQNRAERPGLGQGRVGWHHAPLDTGAMHEAAQQLLGTHDFSSFRAAGC
                     QAKSPVKTLSRLCVTRAGAIVRFDVTADAFLHHMIRNIVGALVDVGAGKRAPGWIGEL
                     LQAADRTRGAPTFAPDGLYFTGADYDARMALPATRRDVPAEFA"
     gene            complement(4205..6901)
                     /locus_tag="VFO53_10500"
     CDS             complement(4205..6901)
                     /locus_tag="VFO53_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HET8876747.1"
                     /translation="MRAKPIFTYSLVAGALAFHGHALALGLGKLTVDSALGQPLSARI
                     ELTSVTKEDLDSLRAKVADPSLYQQNNLTYQATLSRARVSLESSGGSPYLRVTSPVSV
                     QEPFLDLLVELDWASGRVVREYTFLLDPPGMPMAAGAAEPVAPARAGTPARTSAAPAQ
                     RAPSPSASAAAAPSGAGGRASTASAGAVAGGAGSDQYAVRRGDTLQKIATQFKPADAT
                     LEQMLAALFNRNQAAFEGDNMNRLRAGAILTIPSAADTQATSSAEATKIVHMQASDWR
                     AYRDRLAAAAPAAGAAGAREASGRIGAAVEDRTPAAAPGSDKLRVSREAGTGKAGSAA
                     EDAIARDQQLKDAQSRIATLEKTIAEMQRAVELKNQTLAQMQAQAEAAKGGKAETAGA
                     ATAPGAAQNATTPATASPSTTGAPVPAETTAPASTPAATTPPATSSAPANAPAAKAPA
                     PKAAPAPRAEPPGFFADLLSGTPNWALAVAALAILGGIAGIIAARRRKGTKFEDSIIS
                     GTDIKTNTVFGSTGGGVVNTGENSLASDFSREGLGNIDTDEVDPIAEAEVYLAYGRDA
                     QAEEILKDALKKDPQRQEIYLKLLEIHSQHNKPQAFETVASELFAVSHGQGDVWHKAV
                     TLGRQLDPNNPMFQDAAAAQPASAAQQPPAAAPVASASGTASRSTLHATHAFNDGDIS
                     IAPESSTPAKSPADILAGAEDALARSPATPVTAAGMAGQPAQSNVSETMASATAALAG
                     ALAGGKLKPESGSASGVPHFADVANELTTRVDTPAEQPRFDLDFHLDEAERPAPLKRP
                     EAAPQPSLAAASAAASSSTGSLDLDKLDLSFDSSDHPPLEDQTPSVLDGQWHDAATKL
                     DLAKAYQEMGDVDGAREILQEVLHEGDDQQKSEAQSLLSKLA"
     gene            complement(7258..8397)
                     /gene="asd"
                     /locus_tag="VFO53_10505"
     CDS             complement(7258..8397)
                     /gene="asd"
                     /locus_tag="VFO53_10505"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET8876748.1"
                     /translation="MRVGFVGWRGMVGSVLMQRMAAERDFDTVEPLFFSTSSAGGKPP
                     AIASASGSLRDANAPEAFRGLDAIVTCQGGDWTTAMYPRIRDAGFDGYWIDAASAMRM
                     RDDAVIILDPVNRAVIDRALQQDVRNFIGGNCTVSLMVMALAGLLRHDLVEWMTCMTY
                     QAASGAGAQNMRELLQQMGEAHLAAKGLLDDPASSILDIDREVAGILRDERFPTAHTG
                     VPLAGSLIPWIDKDLGNGMSREEWKGGAETNKILGLGENAIPVESICVRVGAMRCHSQ
                     ALTIKLRRDLPLAEIEHLLAGGHEWVRVIPNNRDASIGGLTPAAVTGRLEIPIGRLRK
                     LAMGGDYLGAFTCGDQLLWGAAEPLRRMLRIVQTIGCRTDIGAHA"
     gene            complement(8441..>8746)
                     /locus_tag="VFO53_10510"
     CDS             complement(8441..>8746)
                     /locus_tag="VFO53_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET8876749.1"
                     /translation="SIGMLPSASLDARGKGLYEPIHGSAPDIAGQDRANPLATILSVA
                     MMFRHTFSRNDIATRIENAVRAALRRGLRTPDIAIDAEPVVGTRAMGDGVLAALRNG"
BASE COUNT         1308 a         2953 c         3061 g         1424 t
ORIGIN      
        1 gccaccggag gccgacacac aaacgaacgg cagtcgattc tcgtaggcga cgcgcacccc
       61 gcgcacgaac cgctccccga cgaccgagcc catcgacccg cccatgaaat tgaactcgaa
      121 cacggcgagc acgagcggaa cgctcttgac gctcccctgc atgacgacga gcgcgtcggt
      181 ttcaccggtt tcctccagcg ccgcctcgag ccgctcggga tagcgcttcg tgtccttgaa
      241 cttgaggctg tccaccggca cgacctcgag gccgatctcg aagcggccct cggcatcgag
      301 caactgctcg atgcgctcgc gcgccgggat gcggttgtga tagttgcatt tggggcagac
      361 gagaaggttc ttctcgagat cggtgcgata gagcgtcgcc tcgcacgacg cgcacttgac
      421 ccacagcccc tcggggaccg gcgtcttgcg cgcggcgccg ggcgtgctct tgatgcgtgg
      481 cggcagcagc ttcgtaagcc agctcattga acccccacgc ttgcggcttg cgccgctgcg
      541 ctgcccccgt agggggagca atgagcggct tgggacggcc cggcgccgct catctcgaag
      601 tttgctcgcc cggacgcgtc atgcaacgca tgccgcgagc cgtgtatcga gcgcgcgacg
      661 gatcgtcgcg acgaacgcgc ccgcgcgcat tgcggcttcg cccggcgcag cctcttcgat
      721 ttcgacgatc aggcggctgc cgatcaccac cgcctcggcg tgcgccgcga tagcgcgcgc
      781 gctgtcggca tcgcggatgc cgaagccgac gccgaccggc agcgtgatgt gccgtcgtat
      841 ttcgtcgagc ttgcgcgcga cgtcgccggt gtcgagatgg ccggcgccgg tgacgccctt
      901 cagcgagacg taatagacgt agcccgcggc gaggcgggcg atgcgctcga tgcgcgactg
      961 cggcgtggtc ggcgcgatga gaaagatcgg cgcgacgtcg cgtgcgcgaa ggagcgccgc
     1021 gaactcggtg gcttcctcgg gcggatagtc gacgacgatc acgccgtcga cgccggcgtc
     1081 acgcgcgcga tcgacgaaga ccgtcacgcc catcgcctcg atcggattcg cgtagcccat
     1141 gagcacgatc ggcgtatgtg cgttccgttt tcgaaactgc gccacgaggt cgaggacgcc
     1201 ggtgagcgcc acgccctgcg caagcgcgcg ctcggacgcg cgctggatga cgggaccgtc
     1261 ggccattgga tcggagaacg gcacgccgag ctcgatgaca tcggcgcccg cttcggcgag
     1321 cgtgtcgagg atcgccgccg tcgtagcgag cgacggatcg ccggccgtga cgtacgggat
     1381 tagcaccgtt cgaccggcga tgcgttgccg ctcgaagacg cggtcgatgc gattcaaatg
     1441 cgcgctcgga tgttgtccgc tcatcggacc gcgcttcgct cggcaaccgt cgccatgtcc
     1501 ttgtcgccgc ggccggaaag attgacgaga agtatccggt cgcgcggaag cgtgggtgcg
     1561 agcttcgtcg catacgcgag cgcgtgcgcc gactcgagcg cggggatgat gccctcgatg
     1621 cggcagcagg cgtggaacgc ggcgagcgcc tcgtcgtcgg tgatcgacac gtattcggcg
     1681 cgggccgtgt ccttgagcca cgcgtgctcg gggcccacgc ccggatagtc gaggccggcc
     1741 gagatcgaat gcgtctcgat gatctgtccg ttcgcatcct gcagaaggta cgtgcggttg
     1801 ccgtgcagca cgccgggacg tccggcaacg agcgaggccg cgtgacggcc ggtagcgatg
     1861 ccctcgccgg ccgcttcgac gccgacgagc ctcacggccg gattcggaac gtatgcgtga
     1921 aagatgccca ttgcgttgct gcctccgccg acgcaggcga tcacgacgtc ggggtcgcgt
     1981 cccgcggctt cgggcatctg cgtcaggcat tcgcggccga tcacgctctg gaagtcgcgc
     2041 acgagcatcg ggtacgggtg cgggcctgcg accgtgccga tgatgtagaa cgtcgtgtcg
     2101 acgttggtca cccagtcgcg catcgcctcg ttcagcgcgt ccttcagcgt tttcgatccc
     2161 gactcgaccg gcacgaccgt cgcacccagc aggttcatcc ggtagacgtt ctgcgcctgg
     2221 cggcgcacgt cctcggaacc catgtacacg acgcattcca tgccgaagcg cgccgccacg
     2281 gtggccgttg caacgccatg ctgacccgct ccggtctcgg cgatgacgcg gcgcttgccg
     2341 agtcgccgcg cgagcagcgc ctggccgatg cagttgttga tcttgtgcgc gccggtgtga
     2401 ttgaggtcct cgcgcttgag gaagatctgc gcgccgccca gctcgcgcga ccagcgctcg
     2461 gcgtgataca cgggcgacgg ccgcccgacg tagtgctcga gctcgtgctc gaactcggcg
     2521 acgaactgcg gatcgtcgcg gtagcgtgcg tattgcaccg taagctcgtc gagcgcaccg
     2581 atcagcgttt cggcgacgaa aaccccgcca tagggtccga agtgcccgcg ctcgtcgggc
     2641 aggtcacgta ctcgggaaag ctccgccatc tgcactgcgc actccttgca cgaacgccct
     2701 gatgcgcgtt gcatccttga caccgcggcg cggcgtgccg tcgctcgcgc gctcctcgac
     2761 acccgacgac acgtcgacgg cccacggcgc aaccgcgcgg attgcgcgtt cgacgttgtc
     2821 ggcgtcgagt ccgccggaaa gaatcaacgg cgcacgcaaa cgggcacgcc agtccgcgga
     2881 gagccgtctc caatcgaaca cgcggccggt gccgccgggt agatcgcccg cgcggaaagc
     2941 atcgaacagc aggcccgcgg cgtcgtcgta tggagacagc gattctagca aatcgacgtc
     3001 gtccctgacc gcgatcgcct tcacgtagcg gcgcccgaac gcgcggcaga agggcgccgg
     3061 ctcgctgccg tgaaactgca acacgtcgat cggcaccgcc gcgagcgcct cgcgcacggc
     3121 ctgcgcttcg gggtcgacga agaggccgac aacgctcacg tacggcggca gcgcggcgac
     3181 gatcgacctc gcctgcgcgt gcgtgacgac gcgcggcgtg cccggccaga aaacgaggcc
     3241 gatcgcgtcg gcgcccgcat cggcggccgc gcgcgcatcg ccggcacgcg tgatgccgca
     3301 gattttcacg cgcgtgcgcg gtcgctcggc gttgggggtc acgtggccgg cgagcagcgt
     3361 gaagaccgct tcatgcgaat tccgcgggaa cgtcgcgccg cgttgcgggg agtgccatgc
     3421 gcgcatcgta atccgcgccg gtgaagtaca ggccatcggg tgcaaacgtc ggcgcgccgc
     3481 gtgtgcggtc ggctgcctgc agcagttcgc cgatccatcc gggcgcgcgc ttgcctgcac
     3541 cgacgtccac gagcgcgccg acgatgttgc ggatcatgtg atgcaggaac gcatcggccg
     3601 tgacgtcgaa gcgcacgatc gcaccggcac gcgtcacgca cagccgcgac agcgtcttca
     3661 ccggcgactt cgcctggcaa cctgccgcgc ggaacgacga gaaatcgtgc gtgccgagca
     3721 gctgctgcgc cgcttcgtgc atcgcgccgg tgtcgagcgg cgcgtggtgc cagccgacgc
     3781 ggccttgccc gagtcccggg cgctccgcgc ggttctgcag cacatacgtg tagtgacgag
     3841 cgatcgcgcc aaagcgcgca tggaaatcgg acggcacgcc ggcgcaccac aggaccgcca
     3901 cggaaggcgg caaatgcgcg ttgacgccac gcacccacgc cgtcgacggc cgctcgaccg
     3961 ggacgtcgaa gtggacgatc tgcgacgtcg cgtgcacgcc ggcgtcggta cgtcccgcgg
     4021 gaatggcgcg caccggcacg cccgcaatcg ccgcaagcgc gcgctcgagg gcatcctgga
     4081 tgccacaacc ggaagactgc gactggaagc cgcagtagcc gcttccgtcg tattcgagtg
     4141 cgagggcgcg gcgcatggga ggcccgcgca aagcctggcg cgcagcactt gccctatgcg
     4201 gaaactacgc gagcttcgac aacagcgact gcgcttccga tttctgctgg tcgtcgccct
     4261 cgtgcaggac ctcctgcagg atttcgcgcg ccccgtcgac gtcacccatt tcctgatagg
     4321 ccttggcgag atcgagcttc gtcgccgcgt cgtgccactg accgtcgagc accgacggcg
     4381 tctggtcctc gagcggggga tggtccgacg aatcgaacga caggtcgagc ttgtcgagat
     4441 cgagcgagcc ggtcgagctc gaagcagccg cggaagccgc ggcgagcgac ggctgcggcg
     4501 ccgcttcggg gcgcttgagc ggcgccggac gttcggcctc gtccaggtga aaatcgagat
     4561 cgaagcgcgg ctgctctgcg ggcgtgtcga cgcgggtcgt aagctcgttc gcgacatcgg
     4621 cgaagtgcgg cacgccggat gccgaaccgc tttccggctt cagcttgccg cccgccagcg
     4681 caccggcgag tgcggcggtt gccgacgcca tggtttccga gacgttcgac tgcgcaggct
     4741 gcccggccat gccggcggcc gtcacgggtg tggcggggct gcgggccagc gcatcttcgg
     4801 cgccggcaag gatgtcggcg ggcgacttgg cgggcgtgga cgactcgggc gcgatcgaga
     4861 tgtcgccgtc gttgaatgcg tgggtcgcat gcagcgtcga acgtgaagcc gtgccggaag
     4921 cggacgcgac cggcgccgcc gcggggggct gctgtgccgc ggacgcgggt tgcgcggctg
     4981 ccgcatcctg gaacatcggg ttgttcgggt cgagctggcg cccgagcgtg accgccttgt
     5041 gccagacgtc gccctgtcca tgactgacgg cgaagagctc ggaggcgacc gtttcgaacg
     5101 cctgcggctt gttgtgctgc gaatggatct cgaggagctt gagatagatt tcctggcgct
     5161 gcgggtcctt cttcagcgcg tccttcagga tctcctcggc ctgcgcgtcg cgtccgtagg
     5221 caaggtagac ctcggcttcg gcgatcgggt cgacctcgtc ggtgtcgata ttgccgagcc
     5281 cttcgcggct gaaatcgctg gcgagtgagt tctcgccggt gttgacgacg ccgcctccgg
     5341 tcgagccgaa gacggtgttc gtcttgatgt cggtgccgga gatgatgctg tcctcgaact
     5401 tcgtgccttt gcgccggcgt gccgcgatga tgcccgcgat gccgccgagg atcgcgagcg
     5461 cggcgacagc gagcgcccag ttcggcgtgc ccgagaggag gtcggcgaag aatccggggg
     5521 gctcggccct gggtgccggc gctgctttcg gcgcgggcgc ctttgcggca ggcgcattcg
     5581 cgggcgcgga gctcgtcgca ggaggcgtcg tcgcggctgg cgtggatgcc ggcgcggtcg
     5641 tttcggcggg cacaggtgcg ccggtggtgg acggggaggc ggtcgccggc gtcgtcgcgt
     5701 tctgcgctgc accgggcgcg gtggcggcgc cggccgtttc ggccttgccg cccttggccg
     5761 cctcggcctg cgcctgcatc tgcgccagcg tctggttctt gagctcgacc gcgcgctgca
     5821 tttcggcgat cgtcttctcg agcgtcgcga tgcgcgactg ggcgtccttg agctgctggt
     5881 cgcgcgcgat ggcgtcttcg gccgccgagc cggccttgcc ggtgcccgct tcgcgcgata
     5941 cgcgcaactt gtcggagccg ggcgctgcag ccggcgtgcg atcctcgacg gcggcgccga
     6001 tgcgtccgct ggcctcgcgt gcgcccgcgg cgccggcggc gggcgccgcg gcagcgagac
     6061 gatcgcgata ggcgcgccag tcggatgcct gcatgtggac gatcttcgtg gcttcggcgg
     6121 acgacgtcgc ctgcgtgtcg gctgcgctgg ggatcgtgag gatggcgccg gcacgcaggc
     6181 gattcatgtt gtcgccctcg aacgccgcct ggttgcggtt gaaaagtgcc gcgagcatct
     6241 gctcgagcgt ggcgtcggcg ggcttgaatt gcgtggcgat tttctgcagc gtgtcgccac
     6301 ggcgcacggc gtactggtcg ctgcccgcgc cccccgcgac cgctcccgcc gaggctgtcg
     6361 atgccctgcc gcctgcgccg gacggcgcag ccgcggccga cgcggacggg gatggcgcac
     6421 gttgtgccgg cgccgcgctg gtccgcgccg gcgtccccgc gcgtgcgggt gcgaccggtt
     6481 cggcggcgcc tgctgccatc ggcatgccgg gtgggtcgag caggaacgtg tattcgcgga
     6541 ccacgcggcc cgacgcccag tcgagctcga ccagcaggtc gagaaacggc tcctgcaccg
     6601 agacgggcga cgtgaccctg agatacggcg agccgccgct gctttcgagc gaaacgcgcg
     6661 cccgcgacag cgtcgcctgg taagtgagat tgttttgctg gtacaggctg gggtcggcca
     6721 ccttcgcgcg cagcgaatcg aggtcttcct tcgtgaccga cgtgagctcg atgcgggcgg
     6781 acagcggctg cccgagcgcc gagtcgacgg tcagcttgcc gagccccagc gccagcgcgt
     6841 ggccatggaa cgccagcgcg cctgccacga ggctgtacgt gaagatgggt tttgcgcgca
     6901 tgccgacctc ttggggtttg gctgtggagc tcgggctaac ttaacatcct gcaattagcg
     6961 ccgcaagcta aaccacggta ttagatgggc ttttccgcag tgcgcgcgcc ggcgcgcaac
     7021 acaggttcat gccgctgtgc tattgcggcg caacgggtag cggaacgtga acgcgaatcc
     7081 acaagctatg gtagcgcgag cgcggcgcgc ccgggtgttg catttgcatc cgccggcgct
     7141 ccaaccgatg aaccgacgcg ccgcgaagct gcataaagat gtccaaaatc agcctcgttg
     7201 gcgttaaccg tcggcgttgc ggccaatcgt caagccgcag tcgcttggca tgacggccta
     7261 ggcatgagcg ccgatatccg tccgacaccc gatcgtctgc acgatgcgca gcattcgacg
     7321 caacggctcg gcggctcccc ataaaagctg gtcgccgcag gtaaacgccc cgagatagtc
     7381 gccgcccatg gcgagcttgc gcagccgccc gatcgggatc tccagccgac cggtgacggc
     7441 cgccggcgta aggccgccga tgcttgcgtc ccgattgttc ggaatcacgc gtacccattc
     7501 gtggccgccg gcgagcaggt gctcgatctc ggcaagcggg aggtcgcggc gcagcttgat
     7561 ggtcagtgcc tgcgaatgac agcgcatcgc gccgacgcgc acgcagatcg attcgacggg
     7621 aatcgcgttt tcgccgagcc cgagaatctt gttcgtttcg gccccgccct tccactcctc
     7681 gcggctcatg ccgttgccga gatccttgtc gatccacgga atgaggctgc ctgcaagcgg
     7741 cacgccggta tgcgcggtcg ggaagcgctc gtcgcgcagg atgcccgcga cttcgcggtc
     7801 gatgtcgagg atcgacgagg ccgggtcgtc gaggagcccc ttcgcggcga ggtgcgcttc
     7861 gcccatctgc tgcagcagct cgcgcatgtt ctgcgcaccc gcgccggacg ccgcctggta
     7921 cgtcatgcag gtcatccact cgaccagatc gtggcgcaac agcccggcga gcgccatcac
     7981 catgagactg accgtgcagt tgccgccgat gaagttgcgc acgtcctgct gcagggcccg
     8041 gtcgatcacc gcgcggttga ccgggtcgag gatgatcacc gcgtcgtcgc gcatgcgcat
     8101 cgccgatgcg gcgtcgatcc agtagccgtc gaaacccgcg tcgcgaatgc gcggatacat
     8161 ggcagtcgtc cagtcgccac cctggcaggt gacgatcgcg tcgagcccgc ggaacgcctc
     8221 gggcgcgttc gcatcgcgca gcgatccgct ggccgatgcg atggccggcg gcttgccgcc
     8281 cgcgctcgac gtcgagaaga acagcggctc gacggtgtcg aagtcgcgct cggccgccat
     8341 gcgctgcatc agcaccgagc cgaccattcc ccgccaaccc acgaatccga cgcgcattgt
     8401 cattgcccgt tttccttcac cgatcgccgg tgtgcacggc tcaaccgttt cgcagcgcgg
     8461 cgagcacgcc gtcgcccatc gcacgcgtgc cgacgaccgg ttcggcgtcg atggcgatgt
     8521 ccggcgtgcg caggccgcgg cgcagcgccg cgcgcaccgc attctcgatg cgcgtcgcaa
     8581 tgtcgttccg cgaaaacgtg tgccggaaca tcatcgcgac cgacaggatc gtggcgagcg
     8641 gattcgcgcg atcctgcccg gcgatatcgg gcgccgagcc gtggatcggt tcgtagagtc
     8701 ccttgcccct ggcgtcgagc gaggccgacg gcagcatgcc gatcga
//
LOCUS       DASSVX010000241         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_827216, whole genome shotgun sequence.
ACCESSION   DASSVX010000241 DASSVX010000000
VERSION     DASSVX010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827216"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFO53_10515"
     CDS             complement(<1..349)
                     /locus_tag="VFO53_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8876750.1"
                     /translation="MADAARGTAIDVHGLTKRYGGRAVVDHFSIRVERGTIFGFLGPN
                     GSGKTTTIRMLCGLLTPDEGEGTCLGYDLRRDAAAIKRQVGYMTQRFSLYEDLSIEEN
                     LDFVARMYDVAGRR"
     gene            complement(394..1350)
                     /locus_tag="VFO53_10520"
     CDS             complement(394..1350)
                     /locus_tag="VFO53_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET8876751.1"
                     /translation="MNARLAALACVVAIGGCAERTPAGYPGYVEGEYVRVAAPLSGTL
                     LQLTVERGAEVARDAPLFTLESEQERAARAEAESRVRQAQAALANLEKGRRPPEIAAV
                     RAQLAQAQASLRQSEADLVRTQKLVADKFLPPQKRDESIATRDRDRARVAELSQQVQI
                     ANLPARSDEIAAANAEVKAATDALAQAQWRVAQKSQVAPAAGLVIDTLYRVGEWVPAG
                     ASVVSLLPPANVKIRFYVPEPIVGTLRIGDAVDVRCDGCGAPINAKIRFIAPQAEFTP
                     PVIYSKENRASLVFLVEARPDAFNAALHPGLPVDVAVPTRAK"
     gene            complement(1407..>2483)
                     /locus_tag="VFO53_10525"
     CDS             complement(1407..>2483)
                     /locus_tag="VFO53_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8876752.1"
                     /translation="VAKVRGIADLETSDKAANPALSVRLNNDAASDLGITVQQVGATI
                     RPLLAGDTISYWLGPDGQNYEVNVQLSKEDRRMTSDLANLYLTSNRRGPNGQMQMVPL
                     RQVAELVETSSPQIIKRQDLQRRIGIYASAEGRPAGDVGSDVQKIASAMELPPGYRLA
                     AAGQQQDMQESFSAAVAALALAIVFIYLILASQFASFTQPVAIMASLPFSLIGVFLAL
                     LLTGTTLNIFSIIGFIMLMGLVTKNAILLVDFANRSRRAGGGLYEALLDAGQVRLRPI
                     LMTTAAMIGGMLPLALGLGEGGENQAPMGRAIIGGVITSTLLTLIVVPVIYTYLDGWK
                     ERRKRARERRAAAAQAPLPRGADD"
BASE COUNT          405 a          831 c          816 g          431 t
ORIGIN      
        1 gcctgcgccc ggcaacgtca tacattcgcg cgacgaagtc gaggttctcc tcgatcgaca
       61 ggtcctcgta gaggctgaag cgctgcgtca tgtagccgac ctggcgcttg atcgcggcgg
      121 catcgcgacg caggtcgtag ccgagacacg ttccctcgcc ttcgtcgggt gtcagcaatc
      181 cgcacagcat ccggatcgtc gtcgtcttgc cgctgccgtt ggggccgagg aagccgaaga
      241 tcgtgccgcg ctcgacgcgg atcgagaaat ggtcgacgac ggcgcggccg ccgtagcgtt
      301 tggtgagtcc gtggacgtcg atcgccgtgc cgcgcgctgc gtcggcgatt gctgacgaat
      361 gagcggctgg ctcgctgcgt gacggcggcg ccgtcacttc gcgcgtgtcg gcactgcgac
      421 gtcgaccggc agacccgggt gcaacgccgc gttgaacgca tccggccgcg cctcgacgag
      481 aaacacgagg ctcgcgcgat tctccttgct gtagatgacc ggcggcgtga actccgcctg
      541 cggcgcgatg aagcgaatct tcgcgttgat cggcgcgccg catccatcgc agcgaacgtc
      601 gacggcatcg ccgatccgca acgtgccgac gatcggctcg ggcacgtaaa acctgatctt
      661 gacgttcgcc ggcggcagca acgaaacaac ggaggcgccg gccgggaccc attcgccaac
      721 gcggtacagc gtatcgatca ccaatcctgc cgcaggagcc acctgcgatt tctgcgcgac
      781 gcgccattgc gcctgcgcga gcgcatcggt tgccgccttc acttcggcgt tcgccgcagc
      841 gatctcgtcg ctgcgcgccg gcaggtttgc gatttgcacc tgctggctca actcggccac
      901 gcgcgcccgg tcgcggtcgc gtgtcgcgat ggactcgtcg cgtttttgcg gcggcaggaa
      961 cttgtccgca acgagcttct gtgtgcggac gagatccgcc tcgctttggc gcaatgatgc
     1021 ctgtgcctgc gcgagttgcg cgcgcacggc tgcgatctcc gggggacggc ggcctttctc
     1081 gagattggcg agggccgctt gcgcctgccg tacgcgcgac tccgcttccg cgcgcgctgc
     1141 ccgctcctgc tcgctttcga gcgtgaaaag cggagcgtcg cgcgcgactt cggcgccgcg
     1201 ctcgaccgtc aattgcagca gcgttccgga gagcggcgct gcgacgcgaa cgtactcgcc
     1261 ttcgacgtag cccggatagc cggccggcgt gcgctcggcg catccgccta ttgccacaac
     1321 gcaggcgagc gccgcaaggc gcgcgttcac cggacgtgct ccatccgcgc atcataggtc
     1381 aggaagtggc gcgcgaacct taagcgtcaa tcatcggcgc cgcgtggcag cggcgcctgc
     1441 gctgccgcag cacgacgctc acgtgcacgc ttgcgtcgct ccttccagcc gtcgagatac
     1501 gtgtagatga ccggcacgac aatcagcgtc aagagcgtcg atgtgatgac accgccaatg
     1561 atcgcgcggc ccatcggcgc ctggttctcg ccgccttcac caaggccgag cgcaagtggc
     1621 agcatcccgc cgatcatcgc cgccgtcgtc atcaggatcg gccgcaggcg aacctggccc
     1681 gcatcgagca gcgcctcgta gaggcccccg cccgcgcggc gcgagcggtt cgcgaagtcg
     1741 acgagcagga tcgcgttttt tgtcacgagc cccatcagca tgatgaagcc gatgatcgag
     1801 aagatgttga gcgtcgtgcc cgtcaagagc agtgcgagga atacgccgat cagcgagaac
     1861 ggcaacgacg ccatgatcgc caccggctgc gtgaagctcg cgaactgcga ggcgaggatc
     1921 aggtagatga acacgatcgc gagcgcgagc gctgcgacgg cggcggagaa cgactcctgc
     1981 atgtcctgct gttgtccggc agccgcgagg cgataacccg gcggcagctc catggccgac
     2041 gcgatcttct gcacgtcgct cccgacgtcg ccggcaggac gtccttcggc gctcgcataa
     2101 atgccgatgc gccgctgcag gtcctgcctc ttgatgatct gcggactcga tgtctcgacg
     2161 agctcggcga cctgccgtag cggcaccatt tgcatctggc cgttcggccc acgccgattc
     2221 gacgtcaagt aaagattcgc gagatcggac gtcatccgcc gatcttcttt cgagagctga
     2281 acgttcacct cgtaattctg gccatcgggg ccgagccagt agctgatcgt gtcgccggcg
     2341 aggagcggcc ggatcgtcgc accgacctgt tgcacggtga tgccgaggtc ggatgccgca
     2401 tcgttgttga ggcgaaccga aagggccggg ttcgcggcct tgtccgacgt ctcgaggtcg
     2461 gcgatgccac gcactttcgc gac
//
LOCUS       DASSVX010000242         3624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1043986, whole genome shotgun sequence.
ACCESSION   DASSVX010000242 DASSVX010000000
VERSION     DASSVX010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043986"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2234
                     /locus_tag="VFO53_10530"
     CDS             <1..2234
                     /locus_tag="VFO53_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HET8876753.1"
                     /translation="LDARGIHYWQTARGDTYAQVHWAAGRGVVRDTTTGRIVGAGPHT
                     LPFAYDDLYDATDRKRIAPRLAHLPRPHTLALLRGLLESDGGVSRGSEIYFTTTSQPL
                     AEGVRYQCLRLGVPVSGQRRERDNARVGTRSDGSSIAFKGTTRTFDLRIPAVPEVAAL
                     AGCKAISKRNWITLGGTIFSRVRSVEPITPRPLVVDLKVDIDESYLTAAGLAHNGGKR
                     KGAVCTYLETWHLDIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMKRVMDGGDWTLF
                     SPSDVPDLHDKWGRAFEEAYVRYEEKVARGELRLFKRIPAMQLWRKMLSMLFETGHPW
                     ITFKDACNVRSPQQHVGTVHSSNLCTEITLNTSETEIAVCNLGSVNLLAHMREVDGHY
                     ELDRAKLARTITSAMRMLDNVIDINYYAVSKARNSNLKHRPVGLGIMGFQDCLHLMRL
                     PYASAEAVAFADASMEAVCYHAYWASTALAEERGRYASYSGSLWHRGVLPQDTLALLR
                     DERGGYVDIDESSTLDWPALRERIAAHGMRNSNCVAIAPTATISNIIGVAASIEPEYQ
                     NIYVKSNLSGEFTVVNEHLVRDLKGRGLWDDVMVSDLKYFDGSLAKIDRVPEELRALY
                     ATAFEIEPAWIVEAGARRQKWIDQAQSLNIYMSGVSGKKLDDTYKLAWTRGLKTTYYL
                     RSLAATSAEKSTGRGGELNAVAESGSFGAIAAAGRTSAAATAAEAEPKFCAIDNPECE
                     ACQ"
     gene            2225..3019
                     /locus_tag="VFO53_10535"
     CDS             2225..3019
                     /locus_tag="VFO53_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876754.1"
                     /translation="MSVELPTGAAMTAALAFSIAPVQSEALRERVQVLESRPGVREVL
                     LVTESGQRDADEQHAVIVLGGGGGGFAVTSCDGVPSVHGGLPLSQRQRLANAIGGAVV
                     ALSPPTDRPVMDRSWRLGDDHVADMQSAVHWTRSQWPQSRTWVLGLNNGALSAAVATA
                     SIDDLAGAVLLSCIDEALDRPQKSDRMRVLAVRHRADSDVPHATHSHAGSRKTLVTVH
                     DERQSRSDVAQSGNVVNVPQFNGKEQQVVDVVARWILTGTAPDEIR"
     gene            3330..3593
                     /locus_tag="VFO53_10540"
     CDS             3330..3593
                     /locus_tag="VFO53_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HET8876755.1"
                     /translation="MARYEYAPRHVVDARWLGDYKVWLEFNDGRKGVVDLADELHGEE
                     LEPLRDRDRFSQFYLDYGLASIAWLDGQDFTPEFLYDKLTPMH"
BASE COUNT          633 a         1199 c         1236 g          556 t
ORIGIN      
        1 acctcgacgc acgcgggatt cactactggc aaacggcgcg cggtgacacg tatgcacagg
       61 tgcattgggc cgcgggccgc ggcgtggtgc gcgacacaac gactggtcgc atcgtcggcg
      121 ccggacccca cacgctgccg ttcgcctacg acgacctgta cgatgccacg gatcgcaagc
      181 gcatcgcgcc ccgcctcgcg cacctgccgc gcccgcacac gctcgcgctg ctgcgcgggc
      241 tgctcgagag cgacggcggc gtctcgcgcg gcagcgaaat ctacttcacg acgacgtcgc
      301 agccgctcgc cgaaggggtg cgttatcagt gcctgcgcct cggcgtgccg gtgagcgggc
      361 agcggcgcga gcgtgacaac gcccgtgtcg gcacgcgttc cgacggctcg tcgatcgcat
      421 tcaagggtac gacgaggaca ttcgacctgc gcattcctgc ggtgccggaa gtcgccgcac
      481 tggccgggtg caaggcgatc tccaaacgca actggatcac gctcggggga acgatttttt
      541 cgcgcgtgcg ttcggtcgag cccatcacgc cgcggccgct cgtcgtcgat ctcaaggtcg
      601 acatcgacga gtcgtacctg actgcagccg gcctggcgca caacggaggc aagcgcaagg
      661 gcgccgtttg cacgtacctc gagacctggc acctcgacat cgaggagttc ctcgagctgc
      721 gcaagaacac cggcgacgac cgccgccgca cgcacgacat gaacacggcc aactggattc
      781 ccgacctgtt catgaagcgc gtgatggatg gcggcgactg gacgctgttc tcgccgtccg
      841 acgtgcccga cctgcacgac aagtggggcc gcgcgttcga ggaggcgtac gtgcgctacg
      901 aggagaaggt ggcgcgcggc gagctcaggc tcttcaagcg cattcccgcg atgcagctct
      961 ggcgcaagat gctgtcgatg ctgttcgaga ccgggcaccc gtggatcacg ttcaaggatg
     1021 cctgcaacgt gcgctcgccg cagcagcacg tgggcaccgt gcacagctcc aacctctgca
     1081 ccgagatcac gctcaacacg agcgagaccg agatcgcggt gtgcaacctg ggctcggtca
     1141 acctgctcgc gcacatgcgc gaggtggacg gacactacga gctcgaccgg gcgaagctcg
     1201 cgcgcacgat cacgagcgcg atgcgcatgc tcgacaacgt gatcgacatc aactattacg
     1261 cggtgtcgaa ggcgcgcaac tcgaacctca agcaccgtcc ggtcggcctc ggcatcatgg
     1321 gcttccagga ctgcctgcat ctgatgcgcc tcccgtacgc gtcggccgag gccgtcgcgt
     1381 tcgccgacgc gtcgatggaa gccgtctgct atcacgccta ctgggcatcg actgcgctcg
     1441 ccgaggaacg cggacgctac gcgtcataca gcggcagcct gtggcatcgc ggcgtgctgc
     1501 cgcaggacac gctcgccctg ctgcgcgacg agcgcggcgg ctacgtcgac atcgacgaat
     1561 cgtcgacgct cgactggccg gcgctgcgcg agcgcatcgc cgcgcacggc atgcgcaact
     1621 cgaactgcgt ggcgatcgcg ccgaccgcca cgatctccaa catcatcggc gtggccgcct
     1681 cgatcgagcc cgaataccag aatatctacg tgaagtcgaa tctgtcgggc gaattcaccg
     1741 tcgtcaacga gcacctggtg agggatctca agggccgcgg cctgtgggac gacgtgatgg
     1801 tgtccgacct caagtatttc gacggctcgc tcgccaagat cgaccgcgtg cccgaggagc
     1861 tgcgcgcgct gtacgcgacg gcgttcgaga tcgagcccgc atggatcgtc gaagcgggcg
     1921 cgcgacgcca gaagtggatc gatcaggcgc agagcctcaa catctacatg tccggcgtgt
     1981 cgggcaagaa gctcgacgac acgtacaagc tcgcgtggac acgcggattg aaaacgacgt
     2041 actacctgcg ctcgcttgcc gcgacgtccg ccgagaagtc gacgggacgc gggggcgaat
     2101 tgaacgccgt tgcggaatcg ggcagcttcg gcgcaatcgc ggcggcgggg cgcacgagcg
     2161 cggcggcgac cgcagcggag gccgagccca agttctgtgc gatcgacaat cccgagtgcg
     2221 aggcgtgtca gtagaactgc ctaccggggc ggcgatgacg gcggcgcttg cattctcgat
     2281 cgcgcccgtc caatcggagg cgctgcgcga gcgcgtgcag gtgctcgaat cgcggcccgg
     2341 cgtgcgcgag gtgctgctcg tcaccgagag cggacagcgc gatgccgacg agcagcacgc
     2401 ggtgatcgtg ctcggcggcg gcggcggcgg atttgccgtc acgtcgtgcg atggcgtgcc
     2461 gagcgtgcac ggcggcttgc cactgtcgca gcggcaacgc ctcgccaatg cgatcggcgg
     2521 tgccgtggtg gcgctgtcgc cgccgaccga ccggccggtg atggatcgct cgtggcgcct
     2581 tggcgacgat cacgttgcgg acatgcaaag cgccgtgcac tggacgagat cgcagtggcc
     2641 gcaatcccgc acctgggtgc tcgggctcaa caacggcgcg ctctcggcgg cggtcgccac
     2701 ggcaagcatc gacgacctcg ccggcgccgt gcttctgtcg tgcatcgacg aggcgctcga
     2761 ccggccgcag aaatccgacc gcatgcgcgt gctcgccgtc aggcatcggg cggacagcga
     2821 tgtgccgcac gcgacgcatt cgcatgcggg cagccgcaag acgctcgtca ccgtgcacga
     2881 cgagcggcag tcgcgctccg atgttgcgca gtccggcaac gtcgtgaacg tgccgcaatt
     2941 caacggcaag gaacaacagg tggtggacgt cgtggcgcgg tggattctca ccggcacggc
     3001 gcccgacgag attcggtgaa ccggtgacgc gacagtcagc aatgccttcg agagcatggc
     3061 gcggcggcac cgggacgatg gacccgcgcg aacccagaca ggacgcgatg ttggcgtgga
     3121 cgcgccaggg accagcacga ccgaaacggc cgacaacggc cggcagttgt tctactgggg
     3181 gcgcgcggcc gttgtccggt cgtttgccgc gcgccgattt ttcccaggac tgggttcccg
     3241 gtgtgatgcg accgtccgcc gcccgcgcat tgcggtgcga atccgtggcc ggccgcgcga
     3301 gccatctact tcgcgaaagg aggttcctgt tggcccggta cgagtatgca ccccgacacg
     3361 tggtggacgc acgctggctc ggtgactaca aggtctggtt ggagttcaac gacgggcgca
     3421 aaggcgtggt cgacctcgcg gacgagctgc atggcgaaga gctcgagccg ctgcgggatc
     3481 gcgaccgctt ctcgcagttc tacctcgact acggacttgc gtcgattgcc tggctcgacg
     3541 gccaggattt cacgccggag tttctgtacg acaagctgac gccgatgcac tgaaggaacc
     3601 gcaacctccc ctggcgcagg ccgg
//
LOCUS       DASSVX010000243         7941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1444003, whole genome shotgun sequence.
ACCESSION   DASSVX010000243 DASSVX010000000
VERSION     DASSVX010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444003"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1852)
                     /locus_tag="VFO53_10545"
     CDS             complement(<1..1852)
                     /locus_tag="VFO53_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET8876756.1"
                     /translation="MTSFRCALGAVALSIAVPAGAADNSAEIFELPSVNVVGTAPLPG
                     LGTPLRDVPANVQIFDNRLFARRRSLTLTQFLDLDANSVGAASGQGNAFQQSLNFRGF
                     AASPLLGTPQGVSVFQDGVRINEAFGDVVNWDLLPRSAISTVQLIPGSVPAFGLNTLG
                     GALAIYTKSGAQYPGLAVEASGGMFGRRALEFEYGAATGHLDAFATGNFSDDDGWAQH
                     NASRVRQFFGKVGWEDDRSNLDVSLTLADNALQGAQTLPLSFLDRPRESYTYPDLNQN
                     RLAFIVAKGSRFVGESQLIDANAYYRRFRNTNVSSNVNDDYGAVDPDTGDVRTNPATN
                     DRATIDESSYGGALQWTMRTKLAGRSNQWAIGGGANAATTHFGQASQPAAFTSDRGTI
                     GTGSYADTTVVALRNVNAGVYASDTLSLTGTWTLTASARYDVARVVIEDRSGRDSALN
                     GTNTFARLNPAIGANFNPTPSFTAYASYSEGMRAPTPVELTCADPGAPCKLPNEFLAD
                     PPLAEVVSSTIEAGARGKWRAGTWSAAIYRTDLRNDLAFIASGAGASNAGYFQNVGRT
                     RRQGIELGGSLRIEPVTIALRYNAIDARFRSTFDAASPDNSEADENGGIVV"
     gene            complement(1938..2888)
                     /locus_tag="VFO53_10550"
     CDS             complement(1938..2888)
                     /locus_tag="VFO53_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HET8876757.1"
                     /translation="MKMLWMLRPFLVVAALASGGAVAQPMAYASDEGSGTVSVIDTAS
                     DQVKATITTGGKPRGIAASRDGKRVYASDQTANGLAVIDTASGTVVKRVPLGSSPEGI
                     YLSGDGRWLSAAIEENNQVVIVDTATLAIAKRITMKGKNPEHAVFSPDGKWLYVSAEE
                     ANSVDIIDVARGEVVKSITVGDRPRGIGFLPDGTRAYVGVEQANLIAVIDVPSQTLVT
                     TIKAGVRSNGVAVHPDGKHVYVSAGGDGTVQVIDTATNTISATIPVGKRPWNMALTPD
                     GRKLYVACGRSNAVAVIDTQSNRKVADVPVGQLPWGVAIP"
     gene            2998..5121
                     /locus_tag="VFO53_10555"
     CDS             2998..5121
                     /locus_tag="VFO53_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET8876758.1"
                     /translation="MDERGLHFGIRKSGAIAPLFPLLAIIAPASWAQDAQNTPLVIDP
                     ITVTATRHAQRAFDVAASVDTIDADTIHNAQPMVNLSETLSRIPGVFVANRQNYAQDL
                     QISSRGFGARATFGERGVRIYQDGIPVTMPDGQAQTGSFSLLSAARIEVLRGPFSALY
                     GNASGGVISIFTEDPPDMPYLAFNAGGGSYGTGVLGVKLGARGQYVGGVVAASEFVTD
                     GYRAHSSARRDLTNAKLLIDPSPDTRITLIGNTQFQPETQDPLGLTRAEWSADPRGAD
                     PAAFLFDTRKTINQQQIGAAVDHRFSDAVSLHVDTYGGRRLIRQYLALTGTGATSSGG
                     VPDLDRDYAGVGVRLDWHGAALDRPLTISVGGDFNHQHERRKGFVNNFGDLGDLRRDE
                     DDNVYGNDLYAQAEWAFAPRWTGTVGVRTSNVHYTSDDHYVTAQNPDDSGSRRFEDTS
                     PVAGLVYHVTDDLNAYASYGEGFETPTFAELAYRQGGSGLNFALQPAQSRATEIGLKY
                     RLADRQRLTAAIFNVDTRNEIVVDTATGGRTIYKNASATRRRGAEAAWDGRFGYGIGA
                     HVALTWLRAQFTEPFSTGSSPVPVPSGSRIPAVPSKQAYAELAWAPGRFHGLDTAIEL
                     QYADKLYVNEANTDAAPAYAVMNWRLGFGQTYGAATWKAFVRVNNLFDRNYAGSVIVD
                     DGNGRFFEPAPGRNWFVGANVEVKL"
     gene            5130..5720
                     /locus_tag="VFO53_10560"
     CDS             5130..5720
                     /locus_tag="VFO53_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HET8876759.1"
                     /translation="MRHGVVARYTTTAVVVHWVVAAIVFGQFAFGWLMQQIPKSPPGM
                     RADAFNLHKSIGLCILALMLFRLGWRLAHPAPPLPAMSRWQAYAARANHAALYATLFV
                     MPLAGYLGSVFSGYPVKWFGITLPAWGAKMPAVKDLMSDIHLAASFVLLTLVVGHVAA
                     ALMHAFRRDGVFARMTLAPRADSPRSAAAIPHFNEP"
     gene            complement(5707..6351)
                     /locus_tag="VFO53_10565"
     CDS             complement(5707..6351)
                     /locus_tag="VFO53_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET8876760.1"
                     /translation="MAITDAELATNARLLASARNDDAAGVARALREGAAVDSRNRLGE
                     TGLLIALKKNRADIARALLAAGADVNLAAINGVTPLMAAAYTGDVEMTRTLLERGAGI
                     AAVDRLGKNAITYAAGEGHTEVVRLLLVRGVDPNAIYRNDLTALMWAAGYGHADTARA
                     LIDAGARIELVDNRGKTALDMAREFKHAETVAVLEQALAATPHRAAAGTPATVR"
     gene            6643..>7941
                     /locus_tag="VFO53_10570"
     CDS             6643..>7941
                     /locus_tag="VFO53_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET8876761.1"
                     /translation="MIERNKKLIRVALVAAGLALSLGVGAQETGTADTTVAKPMSKSF
                     APVTQAMLDNAASDSKNWIHSNGSYAQTRYYPGTQVNAGNVGKLKPAFVFQTAVLESM
                     ETSPIVVNGVMFLTTSFNHVYAIDATTGEEYWHYKHKMGPITTFCCGPNNRGVAIDGD
                     RLFMGTLDAKLVALDAKTGKVLWETQIADPDKGYSETMAPTVVEGKVLIGTNGGEYGI
                     RGFVKAFDEKDGHLLWTFYSIPDKGQEGVWAEKDATGRDLHRDIAAEKAALAKDSSFF
                     QTLGGGVWMTPSVDRKTHTIYFVVGNPSPDLYGAIRPGDNLYTDSMVAVDLETGAYKW
                     HSQYVPHDVWDLDAVSATILTQAKDASGKVTDVVIHGGKTGHVYVNDRASGKLIRFSE
                     AMIPQENMWVLPTPTGARMLPGANGGVEWSPMAFNPQYRLA"
BASE COUNT         1330 a         2638 c         2646 g         1327 t
ORIGIN      
        1 ggacgacgat gccgccgttt tcatccgcct cggaattgtc ggggcttgcc gcgtcgaacg
       61 tcgagcggaa gcgtgcatcg atcgcgttgt agcggagcgc gatcgtcacg ggctcgatgc
      121 gcaggctccc gccaagctcg atcccctggc gccgcgtacg cccgacgttc tggaaatagc
      181 cggcgttcga ggcgccggcg ccactggcga tgaaggcgag gtcgttgcgc aggtccgtgc
      241 ggtagatcgc ggccgaccag gtccccgcgc gccacttgcc gcgcgcgccg gcctcgatcg
      301 tcgaagagac gacttccgcg agcggcggat cggcgaggaa ttcgttcggc agcttgcacg
      361 gcgcgcccgg atcggcgcag gtcagctcga ccggtgtcgg agcgcgcatg ccctcgctgt
      421 agctcgcata cgcggtgaac gatggcgtcg gattgaagtt cgcgccgatt gcgggattga
      481 gccgcgcgaa cgtgttcgtg ccgttcaacg cgctgtcgcg accgctccgg tcctcgatca
      541 cgacgcgcgc gacgtcgtag cgcgccgacg cggtgagcgt ccaggtgccg gtgagcgaca
      601 acgtgtccga cgcatacacg cccgcgttga cgttgcgcag cgcgacgacc gtcgtgtcgg
      661 cgtagcttcc ggtaccgatc gttccgcgat cgctcgtgaa cgccgccggc tgcgatgctt
      721 gcccgaaatg cgtcgtcgcg gcgttggctc ccccgccgat cgcccattgg ttcgagcggc
      781 cggcgagctt cgtgcgcatt gtccactgca gggcgccgcc gtacgacgac tcgtcgatcg
      841 tcgcgcgatc gttcgtcgcc ggattggttc gcacgtcgcc cgtatcgggg tcgacagcgc
      901 cgtaatcgtc gttgacgttg ctgctgacgt tcgtgttgcg aaagcggcgg tagtacgcgt
      961 tcgcgtcgat cagttgcgac tcgccgacga accggctccc cttggcgacg atgaacgcaa
     1021 ggcggttctg gttcaggtcg gggtaggtgt acgactcgcg cggacggtcg agaaacgaca
     1081 gcggcagcgt ctgtgcaccc tggagcgcgt tgtccgcaag cgtcaggctg acgtcgagat
     1141 tcgaccggtc gtcctcccag cccaccttcc cgaagaactg ccgaacgcgg ctggcattgt
     1201 gctgcgccca cccgtcgtcg tcggagaaat tcccggtggc gaacgcatcg agatgtccgg
     1261 tcgccgcacc gtactcgaat tcgagcgcgc ggcggccgaa catgccgccg gaagcttcga
     1321 cggccaatcc cggatattgc gcgccgctct tcgtgtagat cgcgagcgcc ccgccgagcg
     1381 tattgaggcc gaacgcgggg accgatccgg ggatcagttg caccgtcgaa atcgccgagc
     1441 gcggaagcag gtcccagttg acgacgtcgc cgaacgcttc gttgatgcgc acgccgtcct
     1501 ggaacaccga cacgccctgc ggcgtgccga gcagcggcga cgcggcgaag ccgcggaaat
     1561 tgagcgactg ctggaacgca ttgccctggc cggatgcggc gccgacgctg ttggcgtcga
     1621 ggtcgaggaa ctgcgtgagc gtcagcgaac gcctgcgcgc gaacaggcga ttatcgaaga
     1681 tctgcacgtt cgcggggacg tcgcgaagcg gggtgccgag tccgggcagc ggtgccgtac
     1741 cgacgacatt caccgacgga agctcgaaga tttcggcaga attgtcggcg gcgccggcag
     1801 gcaccgcaat ggacagtgcg acggcgccga gcgcacaacg gaaggaggtc accgcgatgg
     1861 ggtcagagac gaatttcccg acatgcatgt tttcatcggg acgacagcgc ggggaaattc
     1921 gtctctgacc ccagccctta cggaatcgcc acaccccagg gcaactggcc caccggaacg
     1981 tcggcgacct tgcggttcga ttgggtatcg atcacggcca ccgcgttcga gcgtccgcac
     2041 gcgacgtaga gcttgcgtcc gtcgggggtg agcgccatgt tccatggccg cttgccgacc
     2101 ggaatggtgg cgctgatcgt gttcgtcgcg gtgtcgatca cctgcaccgt gccgtcgccg
     2161 cccgccgaga cgtagacgtg cttgccgtcc ggatgcacgg caacgccgtt ggagcgcacg
     2221 ccggccttga tcgtcgtcac gagcgtttgc gacggtacat cgatcacggc gatgagattc
     2281 gcctgctcga caccgacata cgcgcgtgtg ccgtcgggca ggaagccgat gccgcgcggc
     2341 cgatcgccga cggtgatcga tttgacgacc tcgccgcgcg cgacgtcgat gatgtcgacg
     2401 ctgttcgctt cttcggcgct cacgtagagc cacttcccgt cgggactgaa cacggcgtgc
     2461 tcggggttct ttcccttcat cgtgatgcgt ttcgcgatgg cgagcgtcgc cgtgtcgacg
     2521 atgacgacct ggttgttttc ctcgatggcc gcggagagcc agcgtccgtc gcccgacaga
     2581 tagatgcctt cgggcgagct tcccagcgga acgcgcttca ccacggtgcc gctcgcggta
     2641 tcgatcaccg cgaggccgtt cgcggtctgg tcgctcgcat acacgcgctt gccgtcgcgc
     2701 gacgccgcga tgccgcgtgg tttgccgccg gtcgtgatcg tcgccttcac ctggtcgctc
     2761 gccgtgtcaa tcaccgatac ggtgcccgag ccctcatccg acgcataggc catcggttgc
     2821 gcgaccgcgc cgccggaagc aagcgcggca acgaccagga aaggacgcaa catccagagc
     2881 attttcatgc gcgctccaac aagaaggctt caggggacgt tccccggagt tggcccctgt
     2941 cgatgaatct cgtatgctaa ccaattttcc ccgcccgtac gcggaggagg gaggcgcttg
     3001 gacgaacgcg gcttgcattt cgggatccgt aagagcggcg cgatcgcgcc gctcttcccc
     3061 ctgctcgcga tcatcgcgcc cgcttcgtgg gcgcaggacg cgcaaaacac gccgctggtc
     3121 atcgatccga tcacggtgac cgcgactcgg cacgcccagc gcgcgttcga cgtcgctgcc
     3181 tccgtcgaca cgatcgacgc ggacacgatt cacaacgctc agccgatggt gaatctgtcc
     3241 gaaacgctgt cgcgcatccc cggcgtgttc gtggcgaatc gccagaacta tgcgcaggat
     3301 ctgcagataa gctcgcgcgg gttcggtgcg cgcgcgacat tcggtgaacg cggcgttcgc
     3361 atctatcagg acgggatccc cgtcacgatg cccgacgggc aggcgcagac gggcagcttc
     3421 agcctgctct ccgcagcgcg gatcgaggtc ctgcgcggac cgttctcggc gctgtacggc
     3481 aatgcgtcgg ggggcgtgat ctcgatcttc accgaagatc cgcccgacat gccgtatctc
     3541 gccttcaacg ctggcggcgg cagctacggc accggcgtcc tcggcgtgaa gctcggcgca
     3601 cgagggcaat atgtcggcgg cgtcgtcgcg gcgagcgagt tcgtcaccga cggctatcgc
     3661 gcccactcgt ccgcccggcg cgacctgacg aacgcgaaac tgctgatcga cccgtcgccc
     3721 gacacgcgca taacgttgat cggcaatacg cagtttcaac cggagacgca ggacccgctc
     3781 ggcctgacgc gcgccgaatg gagcgcggac ccgcgcggcg ccgacccggc cgcgttcctg
     3841 ttcgatacgc gcaagacgat caaccagcag cagatcggcg ccgccgtcga ccatcgcttc
     3901 tcggacgccg tgtcgctgca cgtcgacacc tatggcgggc gcagactgat ccgccagtac
     3961 cttgcgctga cggggaccgg tgcgacctcg tcgggcggcg tgcccgacct cgaccgcgat
     4021 tacgccggcg tcggcgtgcg gctcgactgg cacggcgcgg cgctcgatcg gccgctcacg
     4081 atctcggtcg ggggcgactt caatcaccag cacgaacgac gaaagggctt cgtcaataat
     4141 ttcggcgatc tcggcgatct tcgtcgcgac gaggacgaca acgtctacgg caacgacctc
     4201 tatgcgcagg ccgaatgggc gttcgcgccg cgctggaccg gcaccgtcgg cgtgcgcacg
     4261 agcaacgtgc attacacgtc ggacgatcat tacgtcaccg cgcagaatcc cgacgacagc
     4321 ggcagccggc gtttcgagga tacgagcccg gttgcggggc tcgtctacca tgtgacggat
     4381 gatctgaacg cgtacgcgag ctacggcgag ggcttcgaga cgccgacatt cgccgagctc
     4441 gcgtatcggc agggcggcag tggcctcaac ttcgcgctgc aaccggcgca aagccgcgca
     4501 accgagatcg ggctcaagta ccggctcgcg gatcgccagc gactgacggc ggcgatcttc
     4561 aacgtcgaca cgcgcaacga gatcgtcgtc gacaccgcga ccggtggccg gacgatctac
     4621 aagaacgcgt cggcgacgcg gcgtcgcggt gcggaagccg catgggacgg tcgcttcggc
     4681 tacggcatcg gggcgcatgt cgcgctcacg tggcttcgtg cgcaattcac cgaaccgttc
     4741 tccaccggat cgtcgccggt cccggtgccg tcgggctcgc ggatccccgc cgtgccgtcg
     4801 aagcaggcgt acgccgagct cgcgtgggcg cccggccgct tccacggtct cgacacggcc
     4861 atcgagctgc aatatgccga caagctatac gtcaacgagg cgaacaccga cgcagcgccc
     4921 gcgtacgcgg taatgaactg gcgtctcggc ttcgggcaga cgtacggcgc ggcgacgtgg
     4981 aaggcattcg tgcgcgtcaa caacctgttc gatcgcaact acgcgggctc ggtgatcgtc
     5041 gacgacggca acggacgctt tttcgagccg gcgccggggc gcaactggtt cgtcggcgcg
     5101 aacgtcgagg tgaagctgta ggagcgcgca tgcgacacgg tgtggtagcg cgctacacga
     5161 cgacggcggt cgtcgtccat tgggtcgtcg ccgcgatcgt gttcgggcaa ttcgcgttcg
     5221 gatggctgat gcagcagatt ccgaagagcc cgcccggcat gcgcgccgac gcgttcaacc
     5281 tgcacaagtc gatcggcctg tgcatcctcg cactgatgct gttccggctc ggctggcgcc
     5341 tcgcgcatcc ggcgccgccg ctgcccgcga tgtcgcgctg gcaggcgtac gccgccaggg
     5401 cgaaccatgc cgcgctgtac gcgacgctat tcgtgatgcc gcttgccggt tacctgggct
     5461 ccgtcttcag cggctacccc gtcaaatggt tcggcatcac actgcccgcg tggggggcaa
     5521 agatgccggc ggtgaaggat ctgatgagcg acatccatct cgcggcgagc ttcgtgcttt
     5581 tgacgctcgt cgtcgggcac gttgccgcgg cgctgatgca tgcgttccgc cgcgacggcg
     5641 tcttcgcgcg gatgacgctg gcgccgcgcg ccgactcgcc gcgcagcgca gcggcgatcc
     5701 cgcacttcaa cgaaccgtag cgggggtgcc tgcggcggct cgatgcggcg tcgcggcgag
     5761 cgcctgctcg agcacggcca ccgtttccgc gtgcttgaac tcgcgcgcca tgtcgagcgc
     5821 ggtcttgccg cgattgtcca cgagctcgat gcgcgccccc gcgtcgatca gcgcccgcgc
     5881 ggtgtcggca tggccatacc cggcggccca catcagcgcg gtgaggtcgt tgcggtagat
     5941 ggcattcggg tcgacgccgc gcacgagcag cagccgcacg acctccgtgt gtccttcacc
     6001 cgccgcatag gtgatcgcgt tcttgccgag gcgatcgacg gcggcgatgc cggcgccgcg
     6061 ctccagaagc gtgcgcgtca tctcgacgtc gccggtgtag gcggcggcca tcagcggcgt
     6121 cacgccattg atcgcggcga ggttcacgtc ggcgcctgcg gcgagcagtg cgcgggcgat
     6181 gtccgcgcgg ttcttcttca acgcgatcag caatcccgtt tcgccgagac ggttgcgcga
     6241 atcgaccgcc gcgccttcgc gcaacgcgcg cgcaacgccg gccgcgtcgt cgttgcgcgc
     6301 tgacgcgagc agacgtgcgt tggtcgcaag ctcggcatcg gttatcgcca tggcgggcat
     6361 cgtggcaaga agcattgcaa cgaatgcgag cgcggcagtc gcccacgcga ttctgcagcg
     6421 cgtaccgagc gacgcccgcg gcgtgtcttc aggatcgatc gatgtcattc cacactctcc
     6481 aatgcccgcg ctccaggggt tgcccggacg ctattgcggt gcacaatggc gtcgattgca
     6541 acttgcgggg aaacgcacta taacggcgtt tgccgaggcg tcacaaccgt cctcgcaaca
     6601 tgtttccgac cgtcctaaaa acaaggcacc tggaggcctc agatgatcga acgcaacaag
     6661 aagctcatcc gcgtcgcgct ggtggcggcc ggccttgcgc tgtcgcttgg cgtcggcgcg
     6721 caggaaacgg ggacggcgga cacgacggtc gccaagccga tgtcgaagag tttcgcaccg
     6781 gttacgcagg cgatgctcga caacgcggca tccgattcga aaaactggat ccactcgaac
     6841 ggcagctacg cccagacgcg ctactacccc ggcacgcaag tcaacgcagg caacgtcggc
     6901 aagctcaagc ccgcgttcgt cttccagacc gcggtgctcg agtcgatgga aacgtcgccg
     6961 atcgtcgtca acggcgtcat gttcctgacg acgtcgttca accacgtcta cgcgatcgac
     7021 gccaccaccg gcgaggagta ctggcactac aagcacaaga tgggaccgat cacgacgttc
     7081 tgctgcggtc ccaacaatcg cggcgtggca atcgacggcg atcgcctgtt catgggcacg
     7141 ctcgacgcga agctcgtcgc gctcgacgcg aagaccggca aggtgctgtg ggaaacgcag
     7201 atcgccgatc ccgacaaggg ctattcggaa acgatggcgc ccaccgtcgt cgagggcaag
     7261 gtgctgatcg gcacgaacgg cggcgaatac ggcatccgcg gcttcgtcaa ggcgttcgac
     7321 gagaaggacg gccacctgct gtggacgttc tacagcattc ccgacaaggg ccaggaaggg
     7381 gtgtgggccg agaaggatgc gaccggccgc gacctgcatc gcgacattgc ggccgagaag
     7441 gcggcgctcg ccaaggattc gtcgttcttc cagacgctcg gcggcggcgt atggatgacg
     7501 ccgtcggtcg accgcaagac gcacacgatc tacttcgtcg tcggcaaccc gtcgcccgac
     7561 ctctacggtg cgatccggcc cggcgacaac ctgtacaccg actcgatggt tgccgtcgat
     7621 ctcgaaaccg gcgcctacaa gtggcactcg cagtacgtgc cgcacgacgt gtgggacctc
     7681 gacgccgtca gcgcgacgat cctgacgcag gcgaaggatg cgagcggcaa ggtcaccgac
     7741 gtcgtgatcc acggcggcaa gaccggccat gtctacgtca acgatcgcgc gtcgggcaag
     7801 ctgatccgct tttccgaggc gatgattccg caggagaaca tgtgggtgct gcccacgccc
     7861 acgggggcgc gcatgctgcc cggcgccaat ggcggtgtcg agtggtcgcc gatggcgttc
     7921 aacccgcagt accggctggc c
//
LOCUS       DASSVX010000244         5306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1627221, whole genome shotgun sequence.
ACCESSION   DASSVX010000244 DASSVX010000000
VERSION     DASSVX010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627221"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VFO53_10575"
     CDS             complement(<1..1268)
                     /locus_tag="VFO53_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HET8876762.1"
                     /translation="MHAGRTPYFPQWNIRTEGVIAPDERLPAPQTLALGLQHVVAMFG
                     STVLAPLLMGFNPNVAILFSGVGTLIFFVVVGARVPSYLGSSFAYIAVVIAATGYAGS
                     GPNANLGVALSGIIASGVVYLVIGLVVMAIGYRWIDRLMPPVVTGSVVAVIGLNLAPI
                     AVKGISTAPLDTWIGLATIVLVGLIAVRAPGLARRLPILLGGIGGYLVYALCTNVLGL
                     GKPIDFSAVAGAAWIGLPSLTAPVWNAQAIGLIAPVAVVLVAENLGHIKAVAAMTGRN
                     LDAYLGRAFVGDALATIVAGSGGGTGVTTYAENIGVMAVTKVYSTLLFVVAAIFAIVL
                     GFSPKFGALIATIPGPVLGGLAVVVFGLITATGGRIWVQNRVDFSRARNLVTAAVTLT
                     FGAGDLGIRLGGFTLGGIGTATFGAIILYQL"
     gene            1435..2202
                     /gene="modA"
                     /locus_tag="VFO53_10580"
     CDS             1435..2202
                     /gene="modA"
                     /locus_tag="VFO53_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET8876763.1"
                     /translation="MTAAGAARAADIPNIAAASSLQFALTDIAKEFKRESGHDVRLAF
                     GSSGNFRRQIADGAPFEVFLSADAGYAEALVREQRTIGPGVVYAVGRLALFVPIGSPI
                     KADAGLRDVAAALSDGRLRKFAIANPALAPYGRAAQQVLEHAGLWPRIEPHLVLGENI
                     SQAAQFAASGAAQAGIVAYSLIGEPGVAARGHAVLLPATDHAPLDQRMVILKGAGTTA
                     REFAAFILTPGAQAIFARYGLEPQVREPASASPRAPT"
     gene            2205..2873
                     /gene="modB"
                     /locus_tag="VFO53_10585"
     CDS             2205..2873
                     /gene="modB"
                     /locus_tag="VFO53_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HET8876764.1"
                     /translation="MDWQALTLSLRLAGCTVVILLPVGILAGRALAWHRFPGRRLAEA
                     ALALPLVLPPTVLGYYLLSAFGTRGTLGRAFDALVGHPLAFSFAGLLVASVIFNLPFA
                     IQPMQRAFEAIPVDVRPAAACCGMSPWRIFADIELPLAWPGVLSALVLTFAHTLGEFG
                     VVLMVGGNIPGETRTLAIAIYDRVQAFDTRAAGLMAAVLLVLSLFAIAIAYSTQWRAE
                     PARG"
     gene            2866..3996
                     /locus_tag="VFO53_10590"
     CDS             2866..3996
                     /locus_tag="VFO53_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876765.1"
                     /translation="MDERGLSVRVSQPLPVPLDASFDCAAGEVLALTGPSGSGKSTLL
                     RCIAGLERGARGTIACGGSVWLDSGRRINATPQARSVGLVFQHYALFPHLSALGNVQA
                     ALGHRSRGIRAARARELLASVHLAGLEHRRPSQLSGGQQQRVAMARALARDPQALLLD
                     EPFAAVDRVTRQKLYRELAELRRSLAMPIVLVTHDLDEAAMLADRMCILHRGRTLQIG
                     TPQDVMTRPADALVARLVDLRNVFEATVQGHEPAQGRTVIDWQGVVLEVAHAPACAPG
                     TRVNWTVPVSGIVLHRRDRPSRGEHENPVSGIVRECVTLGDDTTIGIRVPVANDALVT
                     LRVPAHVARRNGIAVGVDARVSLLADAIHVMPWREMRAAPDA"
     gene            4101..5048
                     /gene="gshB"
                     /locus_tag="VFO53_10595"
     CDS             4101..5048
                     /gene="gshB"
                     /locus_tag="VFO53_10595"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HET8876766.1"
                     /translation="MDFAFVLDPLPLLKAYKDTSVAMMRALAARGHRVYALEQSVISW
                     RDGATRARVRSLTLHGDDHDWFTAGDEEDRALKDFTAILMRKDPPFDMEYVYSTYLLE
                     AAEREGARVFNRPRAIRDFNEKFAIADFREFIAPTLVTRDEARIGAFIDEHRDVIVKP
                     LDGMGGASVFRVREADPNRNVIVETVSQHGARTVMAQRFIPDIVDGDKRILLIAGEVV
                     PYSLARIPKPGETRGNLAVGGRGVAQPLSPRDREIGEALAAPLWRAGLLVTGIDVIGP
                     YLTEVNVTSPTCFVEISEQSGFDVGERFASALVRACESR"
     gene            5045..>5306
                     /locus_tag="VFO53_10600"
     CDS             5045..>5306
                     /locus_tag="VFO53_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HET8876767.1"
                     /translation="MIGVLIVAHGELGQSLVAAVTHVLGARPPQFEVFPVSADDDPSA
                     LLPKARDAVAALDTGEGVAVLSDLYGATPCNLAVKLARPGHVE"
BASE COUNT          787 a         1871 c         1826 g          822 t
ORIGIN      
        1 agctggtaca ggatgattgc gccgaacgtc gcggtgccga tgccgccgag cgtgaagccg
       61 ccgagcctga tcccgaggtc accggcgccg aacgtcagcg tcaccgcagc ggtgacgagg
      121 tttcgcgcac gcgagaagtc gacacggttc tgcacccaga tgcgtccgcc ggttgcggtg
      181 atgagcccga agacgacgac cgcgaggcca cccagcaccg ggccgggaat ggtcgcgatc
      241 agcgcgccga atttcggcga gaagccgagc acgatggcga agatcgccgc cacgacgaag
      301 agcagcgtcg agtacacctt cgtgaccgcc atgacgccga tgttctcggc gtacgtcgtc
      361 acccccgtgc cgccgcccga cccggcgacg atcgtggcga gggcatcgcc gacgaacgcg
      421 cgcccgagat acgcgtcgag attgcgcccc gtcatcgcgg cgaccgcctt gatgtgcccg
      481 agattctcgg cgacgaggac gacggccacc ggggcgatca gcccgatggc ctgcgcgttc
      541 cacacgggcg ccgtcagcga cggaagtccg atccacgctg cgccggcgac cgcactgaag
      601 tcgatcggct tgccgaggcc gagcacgttc gtgcaaagcg cgtagacgag atagccgccg
      661 atgccgccga gcaggatcgg caggcgtcgc gcgagcccgg gtgcccgcac ggcgatgagg
      721 ccgaccagca cgatcgtcgc gaggccgatc cacgtgtcga gcggcgccgt cgagatgccc
      781 ttcaccgcga ttggtgccag attgaggccg atcaccgcga cgacgctgcc ggtgaccacc
      841 ggcggcatca gccggtcgat ccagcgatag ccgatcgcca tcacgacgag accgatcacg
      901 aggtagacga cgcccgaggc gatgatgccc gagagcgcca cgccgagatt ggcattgggc
      961 ccgcttcccg catagcccgt cgctgcgatc acgacggcga tgtaggcgaa gctcgacccg
     1021 aggtagctcg gcacgcgagc gccgacgacg acgaagaaga tcagcgttcc gacgccggaa
     1081 aacaggatcg cgacattcgg gttaaacccc atcaggagcg gcgccagcac cgtcgagccg
     1141 aacatcgcga cgacgtgctg caatccgagc gcgagcgtct gcggtgcggg caggcgctcg
     1201 tcgggcgcga tcacgccctc ggtgcgtatg ttccactgcg ggaaatacgg cgtgcgaccg
     1261 gcgtgcatgg cggctccttc ggatcggccc gcgcattgaa acaccaaacc ggcgccgctg
     1321 tcagcgtgcg ctgcggtgcg tgacacccct ccgggcacgc gctatagtcg gcggttcgtt
     1381 atgtatgcgt gtatacgacg ctttgcagcg ctcgccgtct ttgttatcgg cggattgacc
     1441 gcggccggcg ccgcgcgtgc cgccgatatt cccaatatcg ccgcggcgtc gagcctgcag
     1501 tttgcgctca ctgacatcgc gaaggagttc aagcgggaaa gcggccacga cgtgcgcctc
     1561 gcattcggct cgtcgggcaa cttccgtcgc cagatcgccg atggtgcgcc gttcgaggtg
     1621 ttcctgtcgg ccgacgccgg ctacgccgag gcgctggtgc gcgagcagcg cacgatcggg
     1681 ccgggcgtgg tctatgccgt cgggcggctc gcgctgttcg tgcccatcgg ctcgccgatc
     1741 aaggccgacg ccgggctgcg cgatgtcgcc gccgcgctga gcgacggacg cttgcgcaag
     1801 ttcgccatcg ccaacccggc actcgcgccc tacggccggg cggcgcagca ggtgctcgag
     1861 catgccggtc tgtggccgcg catcgagccg catctggtgc tcggcgaaaa catcagccag
     1921 gccgcgcagt tcgccgccag cggcgcggcg caggcaggca tcgttgccta ttcgctgatt
     1981 ggcgagccgg gcgttgccgc acgcggccac gcggtgctcc ttcccgccac cgatcacgcg
     2041 ccgctcgacc agcggatggt gatcctgaag ggagcgggca cgacggcgcg cgaattcgcg
     2101 gcattcatcc tcacgcccgg ggcgcaggcg atcttcgcgc gttacgggct cgagccgcag
     2161 gtccgcgagc ccgcgtccgc ctcaccccgc gccccaacct gacgatggat tggcaagcgc
     2221 tcactttgtc gctgaggctc gccggctgca cggttgtcat ccttttgccg gtcggcattc
     2281 tcgccggccg cgcgcttgcg tggcatcgct ttccgggccg ccgactcgcc gaggcagcgc
     2341 tcgcgctgcc gctcgtgctg ccgcccacgg tgctcggcta ctacctgctt tcggcgttcg
     2401 gcacgcgcgg aacactcggc cgcgcattcg acgcgctcgt cggccatccg cttgcgttca
     2461 gcttcgccgg attgctcgtc gcctcggtga tcttcaacct gccgttcgcc atccagccga
     2521 tgcagcgcgc gttcgaggcg atcccggtcg acgtgcgtcc ggccgccgcc tgctgcggca
     2581 tgagcccgtg gcgcatcttc gccgacatcg agctgccgct cgcctggccg ggcgtgctct
     2641 cggcgctcgt gctgaccttc gcgcacacgc tcggcgagtt cggcgtggtg ctcatggtcg
     2701 gcggcaacat tcccggcgag acgcgcacgc tcgcgatcgc gatctacgac cgcgtacagg
     2761 cgttcgacac gcgggccgcg ggcctgatgg ccgcggtgct gctggtcctg tcgctgttcg
     2821 cgatcgcgat tgcgtactcc acgcagtggc gcgcggagcc ggcgcgtgga tgagcgcggg
     2881 ctgtcggttc gtgtctcgca gccgctcccc gttccgctcg acgcgtcgtt cgactgcgcc
     2941 gccggcgaag tgctcgcgct gaccgggccc tcgggcagcg gcaagtcgac gctgctgcgt
     3001 tgcatcgccg gcctcgagcg cggcgcgcgc gggacgatcg cctgcggggg aagcgtatgg
     3061 ctcgacagcg gccggcgcat caacgcgacg ccgcaggcgc ggtccgtcgg cctcgtgttc
     3121 cagcactacg cgctgtttcc gcatctcagc gcccttggca acgtgcaggc ggcgctcggc
     3181 caccggtcac gcggcatccg cgccgcgcgg gcgcgcgagc tcctcgcgag cgtgcatctg
     3241 gcgggactcg agcaccggcg accgagccag ctgtcgggtg gccagcagca acgggtcgcg
     3301 atggcacgcg cgctcgcacg cgacccgcag gcgctgctgc tcgacgagcc gttcgccgcg
     3361 gtggatcgcg tcacgcggca gaagctctat cgcgagctcg ccgagttgcg ccgcagcctc
     3421 gcgatgccga tcgtgctcgt cacgcacgat ctcgacgaag cggccatgct cgccgaccgg
     3481 atgtgcatcc tgcatcgcgg gcgcacgctg cagatcggca cgccgcagga cgtgatgacg
     3541 cgccccgccg acgcgctcgt cgcgcgcctc gtcgatctgc gcaacgtttt cgaggcgacg
     3601 gtgcagggcc acgagccggc gcaagggcga accgtgatcg actggcaggg tgtcgtgctg
     3661 gaggttgcac atgccccggc gtgcgcgccc gggacccggg tgaactggac cgttccggtg
     3721 agcgggatcg tgctccaccg ccgcgatcgg ccgtcgcgtg gcgagcacga gaatccggtg
     3781 tccggcatcg tccgcgaatg cgtcacgctc ggtgacgaca cgacgatcgg catccgcgtc
     3841 ccggttgcca acgatgcgct ggtcacgctc cgcgtgccgg cacacgtcgc gcggcgcaac
     3901 ggcattgcgg tcggcgtcga tgcgcgcgtg tcgctgcttg ccgacgcgat acacgtgatg
     3961 ccgtggcgcg agatgcgagc cgcgccggac gcgtgaccac gcgccggcgc gaagcgtcct
     4021 catcaggcga acccgcgggc gcgagcaggc cggtggataa ggtacgattg accgcttcgc
     4081 tcccgcccat ggcctgatgc atggatttcg cgttcgtcct cgacccgctg ccgctgctga
     4141 aggcctacaa ggacacgagc gtcgccatga tgcgcgcgct tgcggcccgc ggccaccgcg
     4201 tgtatgccct cgagcagtcg gtcatctcct ggcgcgatgg cgcaacgcgt gcacgcgtgc
     4261 gttcgctcac cctgcacggc gacgatcacg actggttcac ggcgggcgac gaggaagacc
     4321 gcgcgctcaa ggatttcacg gcgatcctga tgcgcaagga cccgccgttc gacatggagt
     4381 acgtctactc gacgtacctg ctcgaggccg ccgagcgcga aggcgcgcgc gtattcaacc
     4441 gcccgcgcgc cattcgcgac ttcaacgaga agttcgcgat cgccgatttc cgcgaattca
     4501 tcgcgcccac gctcgtcacg cgcgacgaag cgcgaatcgg cgccttcatc gacgagcacc
     4561 gcgacgtcat cgtcaagccg ctcgacggca tgggtggagc gtcggtattc cgggtgcgcg
     4621 aggcggaccc gaatcgcaac gtgatcgtcg aaaccgtttc gcagcacggc gcgcgaacgg
     4681 tcatggcgca gcgcttcatt cccgacatcg tcgacggcga caagcgcatc ctgctgatcg
     4741 ccggtgaggt ggtgccttac tcgctcgcgc gcatcccgaa gcccggtgaa acccgcggca
     4801 acctggccgt tggcggacgc ggcgtggcgc aacccctttc gccgcgcgac cgcgagatcg
     4861 gcgaggcgct cgcggcgccc ctgtggcgcg cgggcctgct cgtcaccggc atcgacgtga
     4921 tcgggccgta cctgaccgag gtcaacgtga cgagcccgac gtgcttcgtc gagatctcgg
     4981 agcagtcggg attcgacgtg ggcgagcgct tcgccagcgc gctcgttcgc gcctgcgaat
     5041 cccggtgatc ggcgtcctga tcgtcgcgca cggcgagctc ggacaaagcc tcgtcgctgc
     5101 ggtcacgcac gtgctcggcg cgcgtccgcc gcagttcgag gtgttccctg tttccgccga
     5161 cgacgatccg agcgcgctgt tgcccaaagc gcgcgacgcg gtagccgcgc tcgacacggg
     5221 cgaaggcgtc gccgtgctgt cggacctcta tggcgcaacg ccgtgcaacc tggccgtcaa
     5281 gctcgcacgg ccgggccacg tcgagg
//
LOCUS       DASSVX010000245         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_977377, whole genome shotgun sequence.
ACCESSION   DASSVX010000245 DASSVX010000000
VERSION     DASSVX010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977377"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFO53_10605"
     CDS             complement(<1..228)
                     /locus_tag="VFO53_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876768.1"
                     /translation="MVAVTHAAPQAAAPQAVSAWLDSVASTYGAPARATLERAIALAR
                     ERCGDAVMPDGESVLARALGTASILAAIRLDV"
     gene            complement(291..1670)
                     /gene="glmM"
                     /locus_tag="VFO53_10610"
     CDS             complement(291..1670)
                     /gene="glmM"
                     /locus_tag="VFO53_10610"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET8876769.1"
                     /translation="MTDTSARQRRFFGTDGMRGRVGDPPITPDTVLKLGWAAGRTLAN
                     ADGARAERPAVLIGKDTRISGYLLEAALEAGLAAAGVDVYLCGPLPTPAIAYLTRALR
                     LSAGIVISASHNPFDDNGIKFFSGGGAKLADDVELAIEQRMDEPLACAPAAELGKAFR
                     VGDAAGRYIEFCKSTFPNERDLRGCKIVVDCAHGAGYHVAPPVFHELGADVVAVGDAP
                     NGTNINAGVGATHPEYLAEQVRANGADFGVGLDGDGDRLVMADSQGRLYDGDQLLYVI
                     ARDYQRRGVLTGGVVGTLMSNLGFEQALTRMGIRLLRAKVGDRYVLEQLLARGWQLGG
                     ENSGHLICLDKHTTGDAIVAALAVLRAVIEQRTTLADATQPVALFPQRLINVPVKRGW
                     DYRGSVAATEAERTAIAALGSSGRVLLRASGTEPVLRVMVEARERSLADEHAEALAHV
                     IATAAGQAL"
     gene            complement(1667..>2535)
                     /gene="folP"
                     /locus_tag="VFO53_10615"
     CDS             complement(1667..>2535)
                     /gene="folP"
                     /locus_tag="VFO53_10615"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET8876770.1"
                     /translation="EQSMTVLVAGRFELDVATPCVMGILNVTGDSFSGDGRLDHAAAI
                     AHGRAMLRDGARIVDVGGESTRPGAAPVDEAEELRRVIPVIEALAAEGACVSVDTMKP
                     SVMREAIAAGASMINDVNALQTPGAVGVAAASNAAVCLMHMQGEPATMQTAPAYADVV
                     TEIRAFLQRRASACIDAGIDKRGIVVDPGIGFGKTPAHNLALLRRLDALAALGFPLLV
                     GISRKATLGLITGREVGERAAASVAAALAAAVRGARILRVHDVRETVDALAVWNAIEP
                     WPASSPSRNTEQ"
BASE COUNT          416 a          870 c          848 g          401 t
ORIGIN      
        1 gacgtcgagg cggatcgcgg cgaggatcga cgcggtgccg agcgcgcgcg caaggacact
       61 ctcgccatcg ggcatgacgg cgtcgccaca gcgctcgcgc gcgagcgcga tcgcgcgctc
      121 gagcgtggca cgcgcgggcg cgccgtacgt cgaggcaacg gaatcgagcc acgcggaaac
      181 cgcctgcggc gcggcagctt gcggcgcggc gtgcgtgacg gcgaccatcg gcgaattata
      241 gttgccaggc gcaagcaata tgcgcggtgc ggattaaagc ctgctggaat ctatagtgcc
      301 tgccccgcgg ctgtcgcgat gacatgcgcg agcgcttcgg catgctcatc ggcgagggac
      361 cgctcgcgcg cctcgaccat gacgcgcaaa acgggctcgg tgcccgacgc gcgcagcagc
      421 acgcggcccg atgagccgag cgcggcgatc gcggtgcgtt ctgcttcggt cgccgccacg
      481 ctgccgcgat aatcccagcc gcgcttgacg ggtacgttga tgaggcgctg cgggaagagc
      541 gcgacgggct gcgtcgcatc ggcaagcgtt gtgcgctgct cgatcaccgc gcgaagcacc
      601 gcgagcgcgg cgacgatcgc gtcgccggtc gtgtgcttgt cgaggcagat caggtgaccc
      661 gagttctcgc cgcccagttg ccagccacgc gcaagtaatt gctccaatac gtaacggtcg
      721 ccgactttgg cgcggagcag tcggatgccc atgcgcgtca acgcctgctc gaagccaaga
      781 ttgctcatca gcgttccaac gacgccgccg gtcaacacgc cgcggcgctg ataatcgcgt
      841 gcgatgacat agagcaattg atcgccgtcg tacaaccgcc cctggctgtc ggccatcacg
      901 agccgatcac cgtcaccgtc caagccaacg ccgaaatcgg cgccgttcgc gcgcacctgc
      961 tcggcgagat attccggatg tgtcgcaccg acgcccgcgt tgatgttggt gccattcggc
     1021 gcgtcgccga ctgcaacgac gtcggcgcca agctcgtgga ataccggtgg cgcaacgtgg
     1081 taaccggcgc catgcgcgca atcgacgacg atcttgcaac cgcgaagatc gcgctcgttc
     1141 gggaacgtgc tcttgcagaa ttcgatgtag cgtccggccg catcgcccac gcgaaacgcc
     1201 ttcccgagct cggccgccgg cgcgcacgcg agcggctcgt ccatgcgctg ctcgatcgcg
     1261 agctcgacgt cgtcagcgag cttcgcacca ccgccggaaa agaacttgat gccgttgtcg
     1321 tcgaacgggt tgtgtgacgc gctgatgacg atgccggcgg atagccgcag cgcgcgcgtc
     1381 agataagcga tcgccggcgt cggcaatggt ccgcacaggt agacgtcgac acctgccgcg
     1441 gccaatcccg cctcgagcgc cgcctcgagc aggtagccgg agatccgcgt gtccttgcca
     1501 atcagcaccg cagggcgctc ggcccgcgcg ccgtccgcgt tcgccagcgt gcggcctgcg
     1561 gcccagccga gcttgaggac cgtgtccggc gtgattggtg gatcgccgac gcgtccccgc
     1621 atcccgtcgg ttccgaaaaa gcggcgctgc cgcgcagagg tatcggtcat tgctcggtgt
     1681 ttcgtgatgg gctggatgca ggccacggct cgatcgcatt ccagactgcc agcgcgtcga
     1741 ccgtctcccg cacgtcgtgc acacgcagga tccgtgcccc gcgcacggct gcggcaagtg
     1801 cagcggcgac gctcgccgcg gcgcgctcgc ccacttcgcg acccgtgatg agtccgagcg
     1861 tcgccttgcg cgaaatgcca acgaggagcg gaaatccaag tgcagcaagt gcgtccagtc
     1921 gacgcagcag cgccagatta tgggcgggcg tcttgccgaa gccgattccg ggatcgacga
     1981 cgatcccgcg tttgtcgatg ccggcgtcga tgcacgctga cgccctgcgt tgcaggaatg
     2041 cccggatttc ggtcacgaca tccgcatagg cgggcgcggt ctgcatcgtc gccggctcgc
     2101 cctgcatgtg catcaggcag acggcggcgt tcgacgccgc ggcgacgccg accgcgccgg
     2161 gtgtctgcag cgcgttgacg tcgttgatca tggacgcgcc agcggcgatc gcttcgcgca
     2221 tcacgctcgg tttcatcgtg tcgaccgata cgcacgcacc ttccgcagcg agcgcttcga
     2281 tgacgggaat cacacggcgc agctcttcgg cttcgtcgac cggcgctgcg ccgggtcgcg
     2341 tcgactcgcc cccgacgtcg acgatacgcg cgccatcccg cagcatcgcg cgcccgtggg
     2401 cgatcgccgc ggcatgatcg agtcgcccgt caccggaaaa cgagtcgccc gtgacgttca
     2461 ggatgcccat gacgcagggc gttgcgacgt cgagctcaaa ccgtccggcg acgagcaccg
     2521 tcatcgattg ctcgt
//
LOCUS       DASSVX010000246         7561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1644053, whole genome shotgun sequence.
ACCESSION   DASSVX010000246 DASSVX010000000
VERSION     DASSVX010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644053"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFO53_10620"
     CDS             complement(<1..416)
                     /locus_tag="VFO53_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HET8876771.1"
                     /translation="MNERAAREVVLVRAIETADGARELWSDADRAWAGRTAAEIVGEK
                     ATDDAFLGRRATLVLERLAERFPKVRALSHVPVLRGWLAPVAAGCAFVIGVAGVDLGS
                     GRIVNLLAPPVLALLVWNLAVYVALAVAATRRRRDA"
     gene            570..1679
                     /locus_tag="VFO53_10625"
     CDS             570..1679
                     /locus_tag="VFO53_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HET8876772.1"
                     /translation="MTTKDAPPAPAKGRGATFNPANRFRADARAPYDDGWPQPGEPGD
                     EPPPPIRTVVTIQRARTIIAHNDSPDVPFTQSVNPYQGCEHGCIYCFARPSHAYLDLS
                     PGIDFETKLFAKPDAANLLRAELAKPGYRCDPIALGTNTDPYQPIEREWKVTRSVLEV
                     LALHEHPFTIVTKSALVERDIDLIAPMAQKRMARVYLSVTTLDRALARRMEPRAAAPH
                     RRLQALRTLADAGIPVGVMVAPVIPQLNDRDLEAILEAAAAHGAQSAGWVLLRLPREV
                     APLFREWLDVHYPLRAGHVMSLVQQMRGGRDYDSAFGTRMRGNGTLAALLERRFAIAC
                     QRLGLNRGREHTGLDVSRFRPPRPDAAERQGELFA"
     gene            complement(1691..4405)
                     /locus_tag="VFO53_10630"
     CDS             complement(1691..4405)
                     /locus_tag="VFO53_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET8876773.1"
                     /translation="MLASAAVHAQTPPGADASTATPPTQPDIRIEVTGSNIKRVENEG
                     ALPVTVITRRDIERSGATTAMELLQQVSANSSLNAVTLGNSVGAVTFSAQTASLRGLG
                     GGRTLVLINGHRLDSFAGEIPGVQGVNLSAIPFQAIERVEILKDGASAIYGSDAIGGV
                     INFITRSDYTGAEATAFYGTPTRGGGGDQWQASGSAGFGDLAKDGWNAFLSAQYNEQK
                     SLDQVDRNFSNTSYRPDIGFFAISPNSNPGRIATGGIGVITNRGGTPRPALSPQDCAP
                     SRFFSDDILGTDCFFDPAVLSGVNMIPHEKTWNFFGNAQFRINADWQAYVTALYARDE
                     SHLVIQPGPVSSLFNYGPQSNIPAAITLQPSSPFYPHSLAAQAGVDGEPLDIWYRTFD
                     NGFRDTTDTNENWEIVSGVKGSWRGMDWDGSLFYARGDTTQRVNNGLQDYTRLLPILN
                     SGNVNFFGPNTPDVIALERSANFTGDAFHGTSKNYGGQVKTSGTAYELPAGALAFAVG
                     VEARKEEFEQTVDPALANGDITGYPGALKSTGLKDRKQMAAFGELDIPIVKTLEGDVA
                     IRYDHYSDFGGTTNPKFGLRWQPTRTLLVRGSYGTGFLAPSLYNLFAPRFGGVTQAGT
                     SDPIRCPVTGDTGVDCNTQFGVVFGGNANLKPEESEQTSVGVVVEPIPGGTFSVDWFK
                     INLKNAIVNGISPSAILGDLAQFGSLVTRAPPDPAFPDLPGRITQIDQTFINLGALRI
                     QGLDVEGRYRTPAQPWGRLSFSIAGTYYLRYDVQNTDLSYSGTVGTALGTVTTGVIPR
                     WKHYASVSWDRGPWSLTLAQTYQTSYTDQNPDIEGNPRTVGSLSLWDMQGSYSGFRNW
                     KLTLGVKNLFDRDPPLSNQLTSFIAGFDPSYYDPRARFVYGSVAYRFK"
     gene            4750..5163
                     /locus_tag="VFO53_10635"
     CDS             4750..5163
                     /locus_tag="VFO53_10635"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HET8876774.1"
                     /translation="MSTGRHVAVWIASIVFFVLGAIWYSVMAAPWVAAIGKSMEELSR
                     EHGASPLPYVVGFVAILVMCYTLAWLMHRLQAATLFDGLRVGAIVAVGFVAATLALNY
                     GFEARSVTLWLINSAYVVVGLALAGAIIGGWKRAA"
     gene            5160..5843
                     /locus_tag="VFO53_10640"
     CDS             5160..5843
                     /locus_tag="VFO53_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020022887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HET8876775.1"
                     /translation="MSATGESAGAPAHDDLGERLRACYSGAVHDVLRAMGVERFVLPP
                     AIKAIAPGTRLAGPVWTVDGHIDRTKSRDETLLGWCTLLAQAPRGHVVVCQPHNHEIA
                     LMGELSAQTLAARGVLGYVVDGGSRDTDLVLAHGFPVFCAFLTPADIVERWIPDRFGE
                     PVTIGDVTIDTGDYLLGDRDGVVIIPRAFAEEAVARTEAVVATESEMRDALVAGMDPV
                     QAYHRYGKF"
     gene            complement(5895..6815)
                     /locus_tag="VFO53_10645"
     CDS             complement(5895..6815)
                     /locus_tag="VFO53_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008897354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8876776.1"
                     /translation="MDSASLARLLVLAALWGGSFLFMRIAAPVLGPTLLIAYRVALAA
                     VFLLGVALLRRRGGRRNAHAQRSPLASDLRKNWRHYVILGASNSAIPFLLFAFAAQTL
                     SASLLSVLNATAPIFAALIASLSRRALPPARTALGLILGVAGVATLVSADALMLQPGA
                     ALALLAALGASLSYGIASTYAKSAGHVAPFSNALGSMWAATLLIAPAVPFFPAHAAAN
                     AGVVWAVLGLGVLCSGVAYLLYFRLIADLGAASALTVTFLIPVFGVLWGRVFLGEPVG
                     RYTVAGALTVIVGTALVTGFSPRMLRMRRA"
     gene            complement(6878..7552)
                     /locus_tag="VFO53_10650"
     CDS             complement(6878..7552)
                     /locus_tag="VFO53_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876777.1"
                     /translation="MNSFYGDSHILFDVSLRVERNEVVALLGRNGAGKSTTLKSLMGV
                     VTPRAGSVLLDGVEIAGMKSHAIAKCGMQLVHEDRRIFGSLDVEENLVLAGLTAPSKW
                     PLDRIYEMFPRLKERRGSRGTDLSGGEQQMLAIARALVRDPKIILLDEPFEGLAPVIV
                     QALMAACRELAAAGQTIVLVEQNLAATLALAQRVYIINNGHIAHEGPADEIKAQPELL
                     QRYLGV"
BASE COUNT         1214 a         2567 c         2563 g         1217 t
ORIGIN      
        1 tgcgcgtcgc gtcgccggcg cgtcgccgcc acggcgagtg ccacgtacac ggcaagattc
       61 cacacgagca gtgccagcac cggcggcgcg aggaggttga cgatccgacc ggaaccaaga
      121 tcgacaccgg cgacgccgat cacgaaggcg cagccggcag ccacgggtgc gagccagccg
      181 cgcagcaccg gcacgtgcga cagcgcccgc actttcggga atcgctcggc gaggcgctcg
      241 agcacgagcg ttgcgcggcg gccgaggaac gcgtcgtcgg ttgccttctc gccgacgatt
      301 tcggccgcgg tgcgcccggc ccacgcgcgg tcggcgtcgc tccagagttc gcgtgcgccg
      361 tcggccgtct cgatcgcgcg gacgagcacg acctcgcgcg cggcgcgctc attcatcgtt
      421 cgcagtcatc gccttcctcc gccgtcgatt atcggccccg ctgtacatcg tgcgcgcgat
      481 gccgtatatt catacggtac gaaaagcgac gctcggcgcg aagcggttcg ccggcggctt
      541 ccctcacgat tttcagcggc cgtcgaacga tgacgacaaa ggatgcgccg ccggcgcctg
      601 ccaagggccg gggtgccacg ttcaatccgg caaatcgctt tcgcgccgac gcgcgcgcgc
      661 cgtacgacga cggctggccg caaccgggcg agcccggcga cgagccgccg ccgccgatcc
      721 gtaccgtcgt caccatccag cgcgcgcgga ccatcatcgc gcacaacgac tcgcccgacg
      781 tgccgttcac gcagtcggtc aatccgtacc agggctgcga gcatggctgc atttactgct
      841 tcgcgcggcc gtcgcacgcc tacctcgacc tgtcgccggg catcgacttc gagacgaagc
      901 tgttcgcgaa gcccgacgcc gcgaatctgc tgcgcgccga gctcgcgaag cctggctacc
      961 gctgcgatcc gatcgcgctc ggcaccaaca ccgatccgta ccagccgatc gagcgcgaat
     1021 ggaaggtcac gcgctcggtg ctcgaagtgc tcgccctgca cgagcatccg ttcacgatcg
     1081 tcaccaagtc ggcgctcgtc gagcgcgaca tcgacctcat tgcgccgatg gcgcaaaaac
     1141 gcatggcgcg cgtctatctg tcggtcacca cactcgaccg cgcgcttgcg cgccggatgg
     1201 agccgcgcgc ggccgcgccg catcgccgcc ttcaggcgtt gaggacgctt gccgacgccg
     1261 gcatcccggt gggcgtcatg gtggcgccgg tcattccgca gctcaacgac cgcgacctcg
     1321 aggcgatcct cgaggctgcg gccgcccatg gtgcgcaaag cgcaggctgg gtgctgctgc
     1381 ggctgccgcg tgaagtcgcg ccgctgtttc gcgaatggct cgacgtgcat tacccgcttc
     1441 gggcggggca cgtcatgagc ctcgtgcagc agatgcgtgg cggtcgcgac tacgattcgg
     1501 cgttcgggac gagaatgcgc ggcaacggca cgctcgccgc gctgctcgaa aggcgcttcg
     1561 cgatcgcgtg ccagcgcctc ggactcaacc gcggccgcga gcacaccggc ctcgacgtgt
     1621 cgcgctttcg tccaccgcgg cccgacgcgg ccgagcggca gggcgagctc ttcgcctagc
     1681 cggtgccccg ctacttgaac cggtacgcga cgctgccgta gacgaaccgc gcgcgcggat
     1741 cgtagtacga cggatcgaac ccggcaatga acgaggtcag ctgattcgac agcggcggat
     1801 cgcgatcgaa caggttcttc acgccgagcg tcagcttcca gttccgaaag cccgaatacg
     1861 acccctgcat atcccacagg ctcagcgagc cgacggtgcg cgggttgccc tcgatgtcgg
     1921 gattctggtc ggtgtacgac gtctggtacg tttgcgcgag cgtgagcgac cacggccccc
     1981 ggtcccacga gaccgacgca tagtgcttcc agcgcgggat cacgccggtc gtcacggtgc
     2041 cgagcgcggt gcccaccgtt ccgctatacg acaggtcggt gttctgcacg tcgtagcgca
     2101 ggtagtacgt gcccgcgatg ctgaacgaca ggcggcccca cggctgcgcc ggcgtgcggt
     2161 aacggccctc gacgtcgaga ccctggatgc gcagcgcgcc cagattgatg aacgtctggt
     2221 cgatctgggt gatgcgcccg ggcaggtcgg ggaaggccgg gtccggcggc gcgcgtgtca
     2281 cgagactgcc gaactgcgcg aggtcgccga ggatcgccga gggcgagatg ccgttgacga
     2341 tcgcgttctt gaggttgatc ttgaaccagt cgacggagaa cgtgccgccg ggaatcggct
     2401 cgacgacgac gccgacgctc gtctgctccg attcctcggg cttgagattc gcgttgccgc
     2461 caaacaccac gccgaactgc gtgttgcagt cgacccccgt atcgccggtg accgggcagc
     2521 gaatcggatc gctggttccc gcctgcgtga cgccgccgaa tctcggcgca aaaaggttgt
     2581 agaggcttgg cgcgagaaag ccggtcccgt acgagccgcg cacgagcaac gtgcgcgtcg
     2641 gctgccagcg caggccgaac ttcggattgg tcgtgccgcc gaaatcgctg tagtgatcgt
     2701 agcggatcgc gacgtcgcct tcgagcgtct tgacgatcgg gatgtcgagc tcgccgaagg
     2761 ccgccatctg cttgcggtcc ttgagtccgg tcgacttcag cgctccgggg tagccggtga
     2821 tgtcgccgtt ggcgagcgcg ggatcgaccg tctgctcgaa ctcttccttg cgtgcctcga
     2881 cgccgacggc gaacgcaagc gcgcccgcgg gcagctcgta cgcggtaccc gacgtcttga
     2941 cctggccgcc gtagttcttc gacgtgccgt ggaatgcgtc gccggtgaaa ttcgccgacc
     3001 gctcgagcgc gatcacgtcg ggcgtgttgg gcccgaagaa gttcacgttg ccgctgttga
     3061 ggatcggcaa gagccgcgtg taatcctgca ggccgttgtt cacgcgctgc gtggtatcgc
     3121 cgcgcgcgta gaagagcgag ccgtcccagt ccatgccgcg ccacgatccc ttgacgccgc
     3181 tcacgatctc ccagttctcg ttggtgtcgg tcgtgtcgcg aaagccgttg tcgaaggtcc
     3241 gataccagat gtccagcggc tcgccgtcca cgcccgcctg cgcggccagc gaatgcggat
     3301 agaacgggct cgacggctgc agcgtgatcg ccgcggggat gttgctctgc ggcccgtagt
     3361 tgaagaggct cgacaccggg cccggctgga tcacgagatg cgattcgtcg cgcgcataga
     3421 gcgcggtcac gtacgcctgc caatcggcgt tgatccggaa ttgcgcgttg ccgaagaagt
     3481 tccacgtctt ctcgtgcggg atcatgttga cgccgctcag gaccgccgga tcgaagaagc
     3541 agtcggtgcc gagaatgtcg tcgctgaaga agcggctcgg tgcgcagtcc tgcggcgaaa
     3601 gcgcgggacg cggcgtgccg ccgcggttgg tgatgacgcc gatgccgccc gtggcaatgc
     3661 gccccgggtt gctgttcggc gaaatggcga agaagccgat gtcgggccgg tacgacgtgt
     3721 tcgagaaatt ccggtccacc tggtcgagcg atttctgctc gttgtactgt gcggacagga
     3781 aggcgttcca gccgtccttg gcgagatcgc caaagccggc gctgccgctc gcctgccatt
     3841 gatcgccgcc gccgccgcgc gtcggcgtgc cgtagaaagc cgtggcttcg gcaccggtgt
     3901 agtcgctgcg cgtgatgaag ttgatgacgc cgccgatcgc gtccgagccg tagatcgccg
     3961 acgcgccgtc cttcagtatc tcgacgcgct cgatcgcctg gaacggaatt gcgctgagat
     4021 tcacgccctg gacgccgggg atctcgccgg cgaagctgtc gagacgatgc ccgttgatca
     4081 gcaccagcgt gcgtccgcca cccagcccgc gcagcgatgc cgtttgcgcg gagaacgtca
     4141 cggcgccgac cgagttgccg agggtcaccg cgttcaggct gctgttcgcc gaaacctgct
     4201 gcagcagctc catcgccgtc gtcgcgccgg agcgctcgat gtcgcgccgg gtgatcacgg
     4261 tgaccggcaa cgcgccctcg ttctccacgc gcttgatgtt ggagccggtc acctcgatac
     4321 ggatgtcggg ctgcgtgggt ggtgtcgccg tgcttgcgtc ggcacccggc ggcgtttgcg
     4381 catgcacggc cgccgaagcg agcaagctcg ctccgagtgc cggtaacaga agcgcggccg
     4441 cattacgttt gcgctgaaat cgcattgttc ctccccggac gccgcgatcg tgccgtccgc
     4501 tgggcaggag ccctcgcggt cgagcggcac ggccgaacgg aagcgatcgt aaccgatccg
     4561 ggcgcggtgt gtgcgcgaaa tctgccggca acccgcaaag ggcaatcatc ccgggtttcg
     4621 accggtcgca aggcgcaccc cgactgcaac gctgcgccgt gcggtacgac agatgcgccg
     4681 gcctccccag gacgcagcgc acctcgcggc tgtggtgaaa tacggccttg cctgacgagg
     4741 gagcgcgccg tgagcacagg aagacatgta gcggtatgga ttgcctcgat cgtgttcttc
     4801 gtgctgggtg ccatctggta cagcgtgatg gcggcaccgt gggtcgccgc gatcggcaag
     4861 tcgatggagg agctatcacg cgagcacggc gcgtcgccgc tcccgtatgt cgtgggcttc
     4921 gtggcgatcc tcgtgatgtg ctacacgctc gcgtggctca tgcatcggct gcaggccgcc
     4981 acgctgttcg acggcttgcg cgtcggcgcc atcgtcgcgg tcggcttcgt cgccgcaacg
     5041 ctggcgctca actacggctt cgaggcccgt tcggtcacgc tgtggctcat caactcggct
     5101 tacgtcgtcg tggggctggc gctcgccggt gcgatcatcg gcggttggaa gcgcgccgcg
     5161 tgagcgcaac gggtgaaagc gccggcgcgc ctgcacacga cgatctcggc gagcggctac
     5221 gcgcctgcta ttcgggtgcc gtgcacgacg tgctgcgcgc catgggcgtc gagcgcttcg
     5281 tgcttccacc cgcgatcaag gcgatcgcgc ccggaacccg ccttgcgggc cccgtatgga
     5341 cggtcgatgg gcacatcgac cgcacgaagt cgcgcgacga aacgctgctt ggctggtgca
     5401 cgctccttgc gcaggcgccg cgcggacatg tcgtcgtgtg ccagccgcac aaccacgaga
     5461 ttgcgctgat gggcgaattg tccgcgcaaa cgctcgccgc gcgcggcgtg ctgggttacg
     5521 tcgtcgacgg cgggtcgcgc gacaccgatc tcgtccttgc gcacggcttc ccggtgtttt
     5581 gcgcctttct cacgccggcg gacatcgtcg agcgctggat tcccgatcgc ttcggcgaac
     5641 cggtcacgat cggcgacgtc acgatcgaca ccggcgatta cctgctcggc gatcgcgatg
     5701 gcgtggtgat cattccacgc gcattcgccg aggaagccgt cgcgcgcacc gaagcggtcg
     5761 ttgcgaccga gagcgagatg cgcgacgcgc tcgttgcagg catggacccc gtgcaggcgt
     5821 accaccggta cggcaagttc tagaggcggc ctcgaaatcg gctgcgcggg ccgaccgacg
     5881 ttccagcgcc cggatcacgc cctgcgcatg cgcagcatcc gcggcgagaa gccggtcacg
     5941 agcgccgttc caacaatgac ggtcagcgcg cccgccaccg tgtagcggcc gaccggctcg
     6001 ccgagaaaca cgcgtcccca cagcacgccg aacactggaa tcaggaacgt gacggtgagc
     6061 gccgacgccg ccccgagatc ggcgatgagc cggaagtaca gcagatacgc gacgccgctg
     6121 cagagcaccc cgaggccgag cacggcccat acgacaccgg cgttcgccgc cgcatgtgcc
     6181 gggaagaatg gcaccgcggg tgcgatcaac agcgtcgcgg cccacatgct tccgagcgcg
     6241 ttcgaaaacg gcgccacgtg gccggccgat ttcgcatacg tcgacgcgat cccgtaagac
     6301 agcgaggcgc cgagcgccgc cagcagcgcg agcgccgcgc ccggctgcag catcaatgca
     6361 tcggcgctca ccagcgtggc gaccccggcc acgccgagaa tcagcccgag cgcggtcctc
     6421 gccgggggca gcgcgcgcct cgacagcgac gcgatcagcg ccgcaaagat cggcgccgtc
     6481 gcattcagca ccgacaacag cgacgccgac agcgtctgcg cggcgaacgc gaaaagcagg
     6541 aacggtatcg ccgaattgct ggcgccgagg atcacgtagt gccgccagtt cttgcgcagg
     6601 tcgctcgcga gcggcgagcg ttgcgcatgc gcgttccggc gcccgccgcg gcggcgcagc
     6661 agcgccacgc cgaggaggaa cacggcggcc agcgccacgc ggtaggcgat cagcagcgtc
     6721 ggaccgagca cgggtgcggc gatgcgcatg aacaggaacg agccgcccca cagcgcggcc
     6781 agcacgagaa gccttgccag gcttgcgcta tccatgcacc gattgtcgat cggatcggcc
     6841 ggaaccgcca atccgattct tgcgctcgta tgagcggtca gacgccgaga tagcgctgca
     6901 gcagctcggg ctgcgccttg atctcgtcgg ccggtccctc gtgcgcgatg tggccgttgt
     6961 tgatgatgta gacgcgttgc gcgagcgcga gcgtcgcggc gagattctgc tcgacgagga
     7021 cgatcgtctg gcccgccgcg gcaagctcgc ggcacgccgc catcagcgcc tgcacgatga
     7081 cgggcgcgag cccctcgaac ggctcgtcga gcaggatgat tttcggatcg cggaccagcg
     7141 cgcgggcgat cgcgagcatc tgctgctcgc cgcccgacag gtcggtgccg cggctgccgc
     7201 gccgctcctt cagccgcgga aacatttcgt agatgcggtc gagcggccat ttcgacggcg
     7261 ccgtgagccc ggcgaggacc aggttttcct cgacgtcgag gctgccgaag atgcgccggt
     7321 cctcgtgcac gagctgcatg ccgcatttgg cgatcgcgtg gcttttcatg ccggcgatct
     7381 cgacgccatc gagcagcacc gagcccgcgc gtggcgtgac cacacccatc aggcttttca
     7441 acgtcgtgct cttgccggca ccgttgcggc cgagcagcgc cacgacctcg ttgcgttcaa
     7501 cgcgcaacga gacgtcgaag aggatgtgcg aatcgccgta gaagctgttc agcccgcgaa
     7561 c
//
LOCUS       DASSVX010000247         3229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_194428, whole genome shotgun sequence.
ACCESSION   DASSVX010000247 DASSVX010000000
VERSION     DASSVX010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194428"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFO53_10655"
     CDS             complement(<1..462)
                     /locus_tag="VFO53_10655"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HET8876778.1"
                     /translation="MLDRDGYRPNVAIVIANGKNLVFWGKRIREHSWQFPQGGINPGE
                     APEQAMYRELREEVGLYPQHVRILGRTRDWLRYEVPQHWVKREWRGSYRGQKQIWYLL
                     RLVGRDNDVSLRATDHPEFDAWRWHDYWVPLESVIDFKREVYRRALMELERF"
     gene            complement(493..1296)
                     /locus_tag="VFO53_10660"
     CDS             complement(493..1296)
                     /locus_tag="VFO53_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET8876779.1"
                     /translation="MLHGQKIGVVMPAYRAARTLAQCFAAIPHDVVDVTLLVDDGSDD
                     ETIAIARRLGIETHRHPRNLGYGANQKTCYRAALDAGADIVVMLHPDYQYEPRLITAM
                     SGMIASGVYDVVIGSRILGNTALRGGMPLYKYVANRWLTALQNLAIGTKLSEFHTGYR
                     AFSRRVLTALPLLANSQDFIFDNQMLVQAHAFGMRIGEISCPTRYFDEASEIDFPRSV
                     VYGIGVLRVSAQYRLWRMRLARPAIFSDAPEFRLPEVSPLGNNSRPVQQ"
     gene            complement(1369..1815)
                     /locus_tag="VFO53_10665"
     CDS             complement(1369..1815)
                     /locus_tag="VFO53_10665"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET8876780.1"
                     /translation="MDDDPPRHRLAPLLRIALQRPRGSGIAHWAHSFSLHVITGFAAV
                     AAHYALMYGLLRAGTEPVPASAIGFGAGALTRFALSYSHIFSPSAGVHRAGLRFAVVI
                     AAQLAANSALLAAFTRAGIDVWPAQVATTVLLTFANYLAYRWWVFR"
     gene            complement(1959..2267)
                     /locus_tag="VFO53_10670"
     CDS             complement(1959..2267)
                     /locus_tag="VFO53_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876781.1"
                     /translation="MNARKTLLALCVAAGSLGTLAPTAASADIYVRVAPPPPRHEVVP
                     VVRQGWVWVPGYWNWNGHRYAWVRGHSIRGRHGSHWVTDRWAEDHGRWRRERGHWDRD
                     "
     gene            2411..>3229
                     /locus_tag="VFO53_10675"
     CDS             2411..>3229
                     /locus_tag="VFO53_10675"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8876782.1"
                     /translation="MELSPVQRRIRDFYFKEALAAPAAVTHVHRQGLVRDQQLFSLEH
                     LRRHLDNPLLDVNYLNLFQNGRTVDLADARLYKVVQRRKIAFTDRRVLQQHLARGAAC
                     VLEGLDILEPDINALATVLDRAHAATFCNATAFFSQKGNEAYRGHLDTDDVLVIHLAG
                     EKRWRLHARQKPRRTQLNEMTDAQMGALEHDVVMRPGDVMFLRSYTPHRVETLSPCSL
                     HVSFDLCDRRPSVEMALQLLLQHYDRDSALPLTPTSDALDKLFELARADPYAADL"
BASE COUNT          568 a         1081 c         1050 g          530 t
ORIGIN      
        1 gaatcgctcg agctccatca gcgcgcgccg atagacctcg cgcttgaagt cgatcaccga
       61 ttcgagcgga acccagtagt cgtgccaacg ccacgcgtcg aactcgggat ggtcggtggc
      121 gcggaggctt acgtcgttgt cgcggccgac gagccgcagc agataccaaa tctgcttctg
      181 cccgcggtag ctgccacgcc actcgcgctt gacccagtgc tgcggtactt cgtagcgcag
      241 ccagtctcgc gtgcggccga gaatcctgac gtgctgcggg taaagcccca cttcctcgcg
      301 cagttcccgg tacatcgcct gttcgggcgc ctcgccggga ttgatgccgc cttgcgggaa
      361 ctgccaggaa tgttcgcgga tccgctttcc ccagaaaacc aggttcttgc cgttggcgat
      421 gacgatggcg acgttcgggc gatagccgtc acggtccagc atcgcggtca ccctgatgca
      481 attcgattga gcttattgtt gcacaggacg actgttgttg cctagcggcg acacctcggg
      541 caaccggaac tccggcgcgt cggagaatat ggccggccgt gcgagccgca tccgccatag
      601 ccggtactgc gcgctcacgc gcagcacgcc gatgccgtaa acgaccgagc gcgggaaatc
      661 gatctcgctc gcctcgtcga agtagcgcgt cgggcacgat atctcgccga tccgcatgcc
      721 gaacgcatgc gcctgcacca gcatctgatt gtcgaaaatg aagtcctgcg aattggcgag
      781 cagcggcagg gcggtcagca cgcggcgcga aaaggcgcgg tagccggtgt ggaactccga
      841 cagcttggtg ccgatcgcga ggttctgcag cgcggtgagc cagcgattcg cgacgtactt
      901 gtaaagcggc atgccgccgc gtagcgcggt attgccgagg atgcgcgagc cgatcacgac
      961 gtcgtagacg cccgatgcga tcatgcccga catggccgtg atgagccgcg gctcgtactg
     1021 ataatcgggg tgcagcatga cgacgatgtc ggcgcccgcg tcgagcgccg cgcgatagca
     1081 cgtcttctgg ttcgcgccgt agccgaggtt gcgcggatgg cggtgcgtct cgatgccgag
     1141 ccgacgcgcg atcgcgatcg tctcgtcgtc gctgccatcg tcgaccagca gcgtgacgtc
     1201 gacgacgtcg tgcggaatcg cggcgaagca ctgcgcgagc gtccttgcgg ctcggtatgc
     1261 cggcatgacg acgccaatct tctgtccatg aagcatcgtc cccgatcccg cccgcgcctc
     1321 accggcgcgt ggcgatcata cgcgcaacgt acgcgatgcg tgagcgcatc agcggaacac
     1381 ccaccaccga taggcgagat aattggcgaa cgtcaggagg accgtcgtcg cgacctgggc
     1441 gggccaaacg tcgatgccgg cgcgcgtgaa tgcggcaagc agcgccgagt tcgcggcgag
     1501 ctgggccgca atcacgacgg caaagcgcag gccggcccgg tgcacgccgg cgctcggcga
     1561 gaagatgtgc gaatacgaaa gcgcgaagcg cgtcagcgcg cccgcaccga acccgatggc
     1621 gctcgccggc acgggttcgg tccctgcgcg gagcaagccg tacatcagcg cgtaatgcgc
     1681 ggcgaccgcc gcgaatcccg taatgacatg cagcgaaaag ctgtgcgccc agtgcgcgat
     1741 gccggagccg cgcggccgct ggagcgcgat tcgcagcagc ggcgcgagac ggtggcgtgg
     1801 cgggtcgtcg tccaagcgaa ggggcagata cgggtcgaag atcgccgcga cacctgccgc
     1861 aaagcgtcct gcaagcgtcc ggcgacgatc ataccggccc gccggcgcaa ccggcgacga
     1921 cggcacgaaa atcgcgccgt cgtcggttta tcgtcgcgtc agtcgcgatc ccagtggccg
     1981 cgctcgcgcc gccagcggcc atgatcctcg gcccagcgat cggtcaccca gtgcgagccg
     2041 tgccggccgc ggatgctgtg tccgcgcacc catgcgtagc ggtgcccgtt ccagttccag
     2101 taaccgggga cccacaccca cccctggcgc accacgggca cgacctcatg gcgcggcggc
     2161 ggcggcgcaa cgcgaacgta aatgtccgcc gatgcggctg tcggtgcgag tgtgccgagc
     2221 gatccggcgg caacgcaaag cgctaagaga gtctttctgg cattcatcgg agcctcgttt
     2281 tcgacagatg accgttcaag attcgcatag tggcatgccg cgctggatcg gcgcgtcgcc
     2341 gatgacggcg taggacgcgc tgaagcctgc tgtaggatgc ggatttcgct tgccgcaagg
     2401 cttcagtcgc atggaacttt cccccgtcca gcgccgcatc cgcgacttct atttcaagga
     2461 agcgctcgcc gcccccgccg ccgtgacaca cgtgcaccgg caggggcttg ttcgcgacca
     2521 gcagctcttc tcgctcgagc atctgcgccg tcatctcgac aatccgctgc tcgacgtcaa
     2581 ttacctgaat ctgtttcaga acggcaggac cgtcgacctc gccgacgcac ggctctacaa
     2641 ggtcgtgcag aggcgcaaga tcgccttcac cgatcggcgc gtgctgcagc agcatctcgc
     2701 gcgcggcgct gcgtgcgtgc tcgaggggct cgacatcctc gagcccgaca tcaacgcgct
     2761 ggcgaccgtg ctcgaccgcg cgcacgccgc gaccttctgc aacgcgacgg cgttcttctc
     2821 gcaaaagggc aacgaggcgt accgcggtca tctcgatacc gacgacgtgc tcgtcatcca
     2881 tctcgcgggc gagaagcgct ggcgtctgca tgcgcgccag aaaccgcggc gaacccagtt
     2941 gaacgagatg accgacgcgc agatgggtgc gctcgagcac gacgtggtca tgcgtcccgg
     3001 cgacgtgatg ttcctgcgca gctacacgcc gcatcgcgtc gaaacgctgt cgccctgctc
     3061 gctgcatgtg tcgttcgacc tgtgcgaccg tcggcccagc gtggaaatgg cactgcagct
     3121 tctgttgcag cactacgatc gcgattcggc gttgccgctc acgccgacga gcgacgcgct
     3181 cgacaagctg ttcgagctcg cccgggccga cccctatgcc gccgatctc
//
LOCUS       DASSVX010000248         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_111018, whole genome shotgun sequence.
ACCESSION   DASSVX010000248 DASSVX010000000
VERSION     DASSVX010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111018"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFO53_10680"
     CDS             complement(<1..563)
                     /locus_tag="VFO53_10680"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8876783.1"
                     /translation="MAVTLYDEPKIDCHCHVLDPAGFAYGADTFYRPSGQEVATALQL
                     AQMHDAYGVRYGLIVGPNSGYEEDNRCLLDAIERGRGRYKGIAVVANGIGQAELLGLR
                     ERGIIGVAFNATHHGVDHYLAAEALLAKLQALDMCVSLQVQHAQLCALAPLFERTGVR
                     LLIDHCGRPSPEAGLHEPGFQELLRLGR"
     gene            complement(571..1380)
                     /locus_tag="VFO53_10685"
     CDS             complement(571..1380)
                     /locus_tag="VFO53_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HET8876784.1"
                     /translation="MNQPIDLDAYFERIRWGGDTHPAYATLAGLLRAHMRHIPFENLD
                     VLLGRGIELDLDALQRKLVRARRGGYCFEHATLFAAVLDALGFAPVRHSARVVLVAPR
                     TEVPRTHMFLTVSLGARRFAVDPGFGGLAPDVPLPLEHAAREPAPDASHWMARDGMYR
                     VMRTRKEGRIIDAWASTLETDNASDFVVANHYVATHPASAFRNRLLLRALTDRGRVSV
                     VNRDVTIQDENGSSTTELADRAALRALLVEHFGFDLPAVESLHVPAIDGWQ"
     gene            complement(1395..1766)
                     /locus_tag="VFO53_10690"
     CDS             complement(1395..1766)
                     /locus_tag="VFO53_10690"
                     /inference="COORDINATES: protein motif:HMM:NF019379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1615 family protein"
                     /protein_id="HET8876785.1"
                     /translation="MTDVSGIPLAPDGDLLRYDDGKPVHDASDTELALHVVASRLRMS
                     ASEMRHDLELARSEAFERSTLYARVLALADTARRTPAPRAAVPTIVLSSPKITRKLTT
                     DWFAHRVDERFVACIRRNGST"
     gene            complement(1763..2158)
                     /locus_tag="VFO53_10695"
     CDS             complement(1763..2158)
                     /locus_tag="VFO53_10695"
                     /inference="COORDINATES: protein motif:HMM:NF019379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1615 family protein"
                     /protein_id="HET8876786.1"
                     /translation="MTADEGRALVRRLLPANLADRAGWATDIYAALAALDIAPTPENI
                     CATVAIAEQESGMRVDPPVPGLARIARKEIESRRVQAGVPKLVLDAALALPSSNGASY
                     GTRLDAVRTASPTTTRGVMRAATPRFSAP"
     gene            complement(2283..2359)
                     /locus_tag="VFO53_10700"
     tRNA            complement(2283..2359)
                     /locus_tag="VFO53_10700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2323..2325),aa:Pro,seq:tgg)
BASE COUNT          412 a          815 c          810 g          370 t
ORIGIN      
        1 cgcccgagcc gcagcagttc ctggaatccg ggctcgtgca gtccggcttc gggcgacgga
       61 cgtccgcaat ggtcgatcag caggcgcacg cccgtacgct cgaaaagcgg cgcgagtgcg
      121 caaagttgcg cgtgctggac ctgcagcgac acgcacatgt cgagcgcctg cagcttcgcg
      181 agcagcgcct ccgcggcgag gtaatggtcg acgccgtggt gcgtcgcgtt gaaggcgacg
      241 ccgatgatgc cgcgctcgcg caagccgagc agttctgcct ggccaatgcc gttcgcgaca
      301 accgcaatgc ccttgtagcg tccgcgcccg cgctcgatcg cgtcgaggag gcagcgattg
      361 tcctcctcgt aacccgagtt cggtccgacg atcagtccgt agcgcacccc gtacgcgtcg
      421 tgcatctgcg caagctgcag cgccgtcgcc acctcctggc ccgacgggcg ataaaacgtg
      481 tccgcgccgt acgcaaagcc ggccgggtcc agcacgtgac agtgacaatc gatcttcggc
      541 tcgtcgtaga gcgtcacggc catgcgcgat tcactgccaa ccgtcgatcg ccggcacgtg
      601 cagcgactcg accgcgggca ggtcgaatcc gaagtgctcg accagcaagg cgcgaagcgc
      661 cgcacgatcg gcaagctcgg tcgtgctcga tccgttctca tcctggatcg tcacgtcgcg
      721 gttcacgacg ctgacgcggc cacgatcggt gagcgcgcgc agcagcagcc gattgcgaaa
      781 cgccgacgcg ggatgcgtcg cgacgtaatg gttggcgacg acgaagtcgc tcgcattgtc
      841 cgtctcgagc gtcgacgccc acgcatcgat gatcctgcct tccttgcgcg tgcgcatcac
      901 ccggtacatg ccgtcgcgcg ccatccagtg ggaggcatcg ggcgccggct cgcgcgccgc
      961 atgctccagt ggaagcggca catccggcgc aaggccgccg aagcccggat cgacggcgaa
     1021 cctgcgcgcg ccaagcgaaa cggtgaggaa catgtgggtg cgcggcacct cggtgcgcgg
     1081 tgcaaccagc acgacgcgcg ccgaatggcg gaccggcgca aaacccagtg cgtcgagcac
     1141 ggcggcgaaa agcgtcgcat gctcgaagca atagccgccg cggcgggcac gtacgagctt
     1201 gcgctgcagc gcgtcaaggt cgagctcgat ccccctgccg agcagcacgt cgaggttctc
     1261 gaacgggatg tggcgcatgt gcgcgcggag cagcccggcg agcgttgcgt acgccggatg
     1321 cgtgtcgccg ccccatcgga tgcgctcgaa ataggcgtcg aggtcgatcg gctggttcat
     1381 cgccgaagag tctgtcacgt cgacccgttg cggcggatac aggcgacaaa tcgctcgtcc
     1441 acgcgatgcg cgaaccagtc ggtcgtcagc ttccgcgtga tcttcgggct cgacagcacg
     1501 atcgtcggta cggcggcgcg cggtgcgggc gtgcgccgcg ccgtgtcggc gagcgccagg
     1561 acgcgcgcgt aaagcgtgct gcgctcgaac gcctcgctcc gtgcgagctc gagatcgtgc
     1621 cgcatctcgc ttgcactcat gcgcagccgg gaagcgacga cgtgcagcgc cagttcagta
     1681 tcgctcgcat cgtgtaccgg cttgccgtcg tcgtagcgga gcagatcgcc gtcgggtgca
     1741 agcggaatgc cggagacatc ggtcacggcg cgctgaaacg cggcgttgcg gctcgcataa
     1801 cgccccgcgt tgtagtcggc gaagcggtcc tgaccgcatc caaccgtgtc ccatacgacg
     1861 cgccattcga cgacggcagc gcgagcgcgg catcgaggac gagcttcggc acgcccgcct
     1921 gcacccggcg tgattcgatc tccttgcgcg cgatgcgtgc gagtcccggt accggcggat
     1981 cgacgcgcat gcccgattcc tgctcggcaa tcgcaaccgt tgcgcagatg ttttccggcg
     2041 tgggcgcaat gtcgagcgcc gcaagcgccg cgtagatgtc cgtggcccaa ccggcgcgat
     2101 cggcgagatt cgccggcagc agccggcgca cgagcgcccg cccctcgtcg gcagtcatcg
     2161 gcggggccga acccgttggc ggcgcattgt tcgttgcgca cccggccacg gtgatcgccg
     2221 ctgcaaacca aagaaaggca atacgcactc gatcggccga ggcggaatga acccagggga
     2281 attggtcggg gtgagaggat tcgaacctcc gacatcttgc tcccaaagca agcgcgctac
     2341 cggactgcgc tacaccccga agcgccgatt atgacattca tcggcggacc gccggtcgcg
     2401 ctcgttg
//
LOCUS       DASSVX010000249        11446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_111160, whole genome shotgun sequence.
ACCESSION   DASSVX010000249 DASSVX010000000
VERSION     DASSVX010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 11446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11446
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111160"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..2343)
                     /locus_tag="VFO53_10705"
     CDS             complement(97..2343)
                     /locus_tag="VFO53_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HET8876787.1"
                     /translation="MPSRPTPLAAALLALFATPWAPLCAASDAPASERDAVPSDPLAA
                     ATIRALPEVNVTRTGAPYAPGVSTIGAGTPAETRDIAQVVTVIPRAVLDAQGAASLSD
                     ALRYVPGITIGGAEGGQIGNNINLRGFSARTDIFLDGSRDRGQYYRDTFFLDAVEVLE
                     GPSSLLFGRGSTGGVVNQSSKVPLATPRNEASVSVGTGDYYRLTGDSNIPLSESAALR
                     LNAMAQDVGGTRDVMRNHDFGVAPSLRLGMGTPTQLTLSALVTHNRDMPDYGLPPVNG
                     GPAPVPYSTFYGLTDDRTVQDVAVTSARVEHALATGITLRNGIQYSRYATDAVETGAS
                     RVGTYADGRFTALPTSSAGNTTSLPASALSVLLGSHDRRIDDETFDNQTEFIADLATG
                     AVRHTLLFGAQFGRDSYDNQAYQRTDPDITGATGLAVVALDRPAYRGASPDALRTTGN
                     RVSSRADTLAAYVDDTAAIGRHLKFVAGLRWDRFDARLSNSINRDNTPGNTAPASASQ
                     TITFTSVRAGAIYQPTRAQSYYASYGTSFDPSLETLMLTSGQQDLAPEENRSFELGGK
                     WDLANGNLSLASALFRVEKTNARSQVATGVYALDGNVRVDGVELSLAGALTPDWQVIG
                     GYTWLDAKIVEASPLDATQGNVPANTPRNALTLWTSYRLTDAWEIGGGLTAMSSRYAS
                     NADVVTAPGFVRFDATLAYHRPNYDLRLNLFNVAGRDYIAALIPSDGGRSVPGIGRTL
                     VATFTYRF"
     gene            complement(2475..3806)
                     /locus_tag="VFO53_10710"
     CDS             complement(2475..3806)
                     /locus_tag="VFO53_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET8876788.1"
                     /translation="MRLHARVLQGIQWSVVGVYALLVITPALLPLPPDDAHWYNSVVV
                     AAQWLFWGLWWPLVIVSMLVLGRTWCGVFCPEGTLTEAASRHGLGRAVPRWMRWGGWP
                     FVAFALTTVYGQLVSVYQYPTATLVVLGGSTLAAIAVGFVYGRGKRVWCRHLCPVNGV
                     FGVLSRLAPVHYRVDAAGWAVNAEHIPVHPVNCAPMVRIKRMTGPSECHMCSRCSGLR
                     GSVSLAARAPNREVAGLAPEDARGWDAVLIVFGMIGLAIGAFAWSATPWFVSLRTIVA
                     DQVLDHGPAWLLADDAPWWLLTHYPDAHDVFTWLDGVLIVGWMGASALIVGGWVAACL
                     GIAALPARGALQRACALSYALAPLAGIGLFIGLSGLTVTLARAEGLHFGSLAVVRAML
                     LCLAAAWSVWLAHGQLRRRVGGVRRAASLLAFAFAVAGVLAAWVPFVFHLG"
     gene            complement(3868..4230)
                     /locus_tag="VFO53_10715"
     CDS             complement(3868..4230)
                     /locus_tag="VFO53_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET8876789.1"
                     /translation="MARRRTIARRHARGAMLLAAALLAAGVAAGAEPMRVYSLSARDG
                     VFAPATIEVPAGTRFKIEIVNAGSGPIEFESRDLRQEKVLAAGARSSVVINGLKPGSY
                     TFFDEFHPDTGKGRIVAK"
     gene            complement(4242..4793)
                     /locus_tag="VFO53_10720"
     CDS             complement(4242..4793)
                     /locus_tag="VFO53_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HET8876790.1"
                     /translation="MPIRRFITSNPSVVFAICLLAASPASALEYPIGKPQIRAGIEVS
                     AVYLQPVPMEPAAMMRAVRDSDIHLEADIRAQADNANGFADGAWMPYLDVRYRIVKQG
                     NAPPLEGALMPMVANDGPHYGDNVKLQGAGRYRLTLTIAPPGANSHFGRHTDRETGVA
                     PWFKPFSLDYEFTYPGLGKTGGY"
     gene            4959..5177
                     /locus_tag="VFO53_10725"
     CDS             4959..5177
                     /locus_tag="VFO53_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET8876791.1"
                     /translation="MYICLCNAITDRQIVEAAHGGARTDEDLAQGLGVGITCGRCRTC
                     AKELLMETLARIAPDASDSRTARDGAAG"
     gene            5174..5650
                     /gene="bfr"
                     /locus_tag="VFO53_10730"
     CDS             5174..5650
                     /gene="bfr"
                     /locus_tag="VFO53_10730"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HET8876792.1"
                     /translation="MKGDPQIVKRLNEVLTNELTAIDQYFLHAKMFSHWGLAHLAARE
                     RHASIDEMKHAESLIERILFLEGLPNLQDLHKLLIGENVREALECDLRLELAAHPTLR
                     AAIADSEAAADYVSRDLFRSILVSEEEHIDWLETQLDLLDKVGLANYQQSQIDAGD"
     gene            5737..7593
                     /locus_tag="VFO53_10735"
     CDS             5737..7593
                     /locus_tag="VFO53_10735"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HET8876793.1"
                     /translation="MRPIEQLESVRAFDDPAASRAVPATLLSEEQRHALDRFVEPLSP
                     EQLIWVGGYLAGLAAAHRTTAHRPPADAAERDESIVTVVYGSQSGNGARVVAEVKAQA
                     EARGLKVRVKPMDEYRPSDLRAEKKLLVVVSTHGEGEPPDNGKEFHEFLHGRKAGRLN
                     GLHYAVLALGDSSYQHFCKTGRDFDERLQSLGAERIHPRADCDVDYDDAAARWIDGVL
                     DALESDATRSEPQPDRRVAIAHAKVTPLHSKRNPFPATAVANINLSGHGSGKEVRHLE
                     LALEGSALNHEPGDSVGILPTNDPAGVAELIEALGLEASSAVDAGNETTTLQFALAHL
                     YEITTLTRPLVERYAELSDAQVLRAMLADERSDALWTYLRGRHLIDLVSEFPVRGLAA
                     PDLLGMLRRLPPRLYSIASSLKANPGEIHATVAAVRYESHGRQRRGVASTFVAERIAE
                     GDQVPIYVEANRNFKLPADPATPIIMVGPGTGVAPFRAFVQEREAIGATGRNWLFYGD
                     QRFTTDFLYQREWQQHLKDGVLTRMDVAFSRDRTPKTYVQHRMKAQARELYAWLEDGA
                     HIYVCGDAERMARDVHQTLIEIVAEHGHLSLDRATEHVQELQRTRRYQRDVY"
     gene            7628..9331
                     /gene="cysI"
                     /locus_tag="VFO53_10740"
     CDS             7628..9331
                     /gene="cysI"
                     /locus_tag="VFO53_10740"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HET8876794.1"
                     /translation="MKEDAEAAGRSAVEHIKDASDYLRGSIVASLANPVTGALADSDT
                     QLIKFHGSYQQDDRDLRDERRRKKLEPLYSFMVRVRAPGGVVTPAQWLVLDELARTHA
                     NGSLRLTTRQSFQVHGIGKPQLKDTIASVNRTLLTTIAACGDVNRNVMCNPNPYQSAL
                     HREVFDWACRISEDLTPHTRAYHEIWLDDRLIAGGEPDHEPIYGRTYLPRKFKIAIAV
                     PPSNDVDVLAHDLGFIAVVDDGRLAGFNVSVGGGMGMTHGEPATYPRLADVIGFCTPD
                     RVLAVTEQVVAVQRDFGDRSDRKHARLKYTIDDRGIGWFTAELASRLGHRLEAPRPFH
                     FETNGDRYGWTEGIDGKLHLTLFIENGRVHDTAECLLMTGLREIAKIHDGDFRLTANQ
                     NLIVASIDPAKRPQIESLLARHCLADADHRSALRLNSMACVALPTCGLAMAESERYLP
                     SLIGKLERVLDECGLANDAITVRMTGCPNGCARPYLAEIAFVGKSLGKYNVYLGGGFA
                     GERLNKLYRSALSEAEILAELTPILQRYAAERRQGERFGDFAIRAGYVNEVRAGREFH
                     A"
     gene            complement(9369..10220)
                     /locus_tag="VFO53_10745"
     CDS             complement(9369..10220)
                     /locus_tag="VFO53_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HET8876795.1"
                     /translation="MATTPCIITVAITGSLPRKKDNPAVPISAAEQIESTHEAFEAGA
                     ALVHIHVRKDDESPTSEPERFAVVVDGIRRHCPGMITQISTGGRSGAGKERGAMLSLR
                     PDMASLATGSCNFPTRVYENPPDLVDWLAGEMVRYGIKPEVEAFDLSMIFKASALRRE
                     GRIKGALHVQFVMGVKNAMPVDRDAFDFYVRTLQRLEPDATWTGAGIGKDQVTLVRWS
                     LELGGHCRTGLEDNVRMNRDTLAPSNAALVRQVAQLCGEYGRRPATIAEARSLLRLPE
                     ASATPHA"
     gene            10344..11177
                     /locus_tag="VFO53_10750"
     CDS             10344..11177
                     /locus_tag="VFO53_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET8876796.1"
                     /translation="MISGKTTLIAHLGYPTDAFKAPMIYNPWFEHAGIDAVVVPMGVR
                     AEDYEAFLPQLFRLTNIRGALVTMPHKVTTVGLLDEVTSTVLIAGSCNAIVRRADGSL
                     LGDMFDGEGFVRGVRRKGRTIAGARALVVGSGGVGSAIAASLAAAGVGELGLFDVDAA
                     VAVALESRLTRHHPRLRVVTGSNDPDGCDIVVNATPLGMKRDDPLPLDTRRLPPGAFV
                     GEVVMTQETTPFLRAALERGCEVQVGTDMLFEQIPAYLEFFGFGSTTADVLRAVAKIE
                     Y"
     gene            11214..>11446
                     /locus_tag="VFO53_10755"
     CDS             11214..>11446
                     /locus_tag="VFO53_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8876797.1"
                     /translation="MATPRARAKPGLALPMMMLLAVTVFASASTVHFQTPMLGRFGVE
                     FAATPAAIGWIPTLSFAGFLAGILFLVPLGDRI"
BASE COUNT         1945 a         3860 c         3852 g         1789 t
ORIGIN      
        1 gcccgtcgac ccacgcgcca tggtcccgtt cgaggagccc gcgcagcgtc gcgagcgccg
       61 gccgatcgag aacatcggga atgcggacga tcacggtcag aaccggtagg tgaacgtcgc
      121 gacgagcgtg cggccgatgc cgggaaccga gcgaccgccg tcggacggaa tcagcgccgc
      181 gatgtagtcg cggccggcga cgttgaagag gttgagccgc aggtcgtagt tcggccgatg
      241 gtaggcaagc gtcgcatcga agcgaacgaa tcccggggcc gtcacgacgt cggcgttgct
      301 cgcataccgc gacgacatcg ccgtgaggcc gccgccgatc tcccatgcat cggtgagccg
      361 gtacgacgtc cacagcgtca gcgcgttgcg cggcgtgttg gccggcacgt tgccctgcgt
      421 tgcgtcgagc ggggacgcct cgacgatctt cgcgtcgagc cacgtgtagc cgccgatcac
      481 ctgccagtcg ggcgtcagcg cgccggcgag cgacagctcg acgccgtcga cgcgcacgtt
      541 gccgtcgagc gcatacacgc cggttgccac ctgcgagcgc gcattggtct tttcgacccg
      601 gaacagggcc gatgcgagcg acaggttgcc atttgcgagg tcccacttgc cgccgagctc
      661 gaacgagcgg ttctcctccg gcgcgagatc ctgctggccc gacgtcagca tcagcgtctc
      721 gagcgacgga tcgaacgacg tcccgtagga ggcgtaatac gactgtgcgc gcgtcggctg
      781 gtagatcgca ccggcgcgta cgctggtgaa cgtgatcgtc tggctcgccg acgccggcgc
      841 ggtgttcccc ggcgtgttgt cgcggttgat gctgttcgac agccgcgcat cgaagcgatc
      901 ccagcgcagt ccggccacga acttcaggtg gcggccgatc gctgccgtat cgtcgacgta
      961 ggcggccagc gtgtcggcac gcgagctcac gcggttgccg gtggtgcgca acgcgtcggg
     1021 cgaagccccg cgatacgcgg gacgatcgag cgcgaccacc gcgagtcccg tggcgcccgt
     1081 gatgtccgga tcggtccgct ggtacgcctg gttgtcgtag ctgtcgcgcc cgaattgcgc
     1141 accgaacagc agcgtgtggc gtacggcgcc ggtggcgagg tcggcaatga actcggtctg
     1201 gttgtcgaac gtctcgtcgt cgatcctgcg gtcgtgactg ccgagcagga cggaaagcgc
     1261 gcttgccggc aggctcgtcg tgttgccggc gctgctcgtc ggcagcgccg tgaaccggcc
     1321 gtcggcgtag gtgccgacgc ggctcgcacc ggtctcgacc gcgtcggtcg cgtagcgact
     1381 gtactgaatc ccattgcgca acgtgatccc ggtcgccagc gcgtgctcga cacgtgcgga
     1441 ggtgaccgcg acatcctgca cggtgcgatc gtcggtcagg ccgtagaacg tcgaataggg
     1501 caccggcgcc gggccgccgt tgaccggcgg caatccgtaa tcgggcatgt cccggttgtg
     1561 cgtgacgagt gcggacagcg tcagctgcgt cggcgtgccc attcccaggc gcagcgaagg
     1621 tgcgacgccg aagtcgtgat tgcgcatcac gtcgcgcgtg ccgcccacgt cctgcgccat
     1681 ggcgttgagc cgcagcgctg cgctttcgga tagcgggatg ttggaatcgc cggtcagccg
     1741 atagtagtcg ccggttccga ccgataccga tgcctcgttg cgcggtgttg caagcggcac
     1801 tttgctcgac tgattgacga cgcccccggt cgatccgcgc ccgaacagca gcgacgaggg
     1861 cccttcgagc acttccaccg cgtcgaggaa gaacgtgtcg cgatagtact ggccgcgatc
     1921 gcgcgacccg tcgaggaaga tgtcggtgcg ggcgctgaag ccgcgcaggt tgatgttgtt
     1981 gccgatctgg ccgccttcgg cgccgccgat cgtgatgccg ggcacgtagc gcaatgcatc
     2041 cgacagcgac gcggcaccct gcgcgtcgag aacggcgcgc gggatgacgg tcacgacctg
     2101 cgcgatgtcg cgcgtttcag cgggtgtgcc ggcgccgatc gtcgagacgc cgggtgcgta
     2161 aggcgcgccg gtgcgggtga cgttgacttc gggaagcgcg cggatcgttg ccgccgccag
     2221 cgggtcggac ggcaccgcgt cgcgctcgct cgccggtgca tcgctcgcgg cgcaaagcgg
     2281 cgcccagggt gtggcgaaca gcgcgagcag cgcggcggcg agaggcgtgg gtcgggacgg
     2341 catcgtcgtt ggcgcgcgcg ggctttcgca tgcgtgcctg gcgttgatcg agaacgcgaa
     2401 tgataatcat tcgtatctac atcggtcaac aactaacgaa catccgacac tgcatcgctg
     2461 cgccgcggcg atcgtcagcc gagatggaac acgaacggga cccacgcggc gagcacgccg
     2521 gcaacggcga aggcgaacgc gagcagcgac gcggcacggc gcacgccgcc gacgcgacgg
     2581 cgcaactggc catgcgcgag ccagacgctc cacgccgccg caagacacag aagcatcgct
     2641 cgcaccacgg cgagcgagcc gaagtgaagt ccttccgcgc gtgcgagcgt gacggtgaga
     2701 ccggacagcc cgatgaatag accaatgccc gccagcggcg ccagcgcata gctcaatgcg
     2761 cacgcgcgct gcagcgcacc gcgcgccggc agcgcagcga tgccaagaca cgcggcgacc
     2821 caaccgccga cgatcagcgc gctggcgccc atccagccga cgatgagcac gccgtcgagc
     2881 caggtgaaga cgtcgtgcgc gtcggggtag tgcgtcagca gccaccaagg cgcatcgtcg
     2941 gcgaggagcc aggcgggccc gtggtcgagc acctggtcgg caacgatcgt gcgcagcgag
     3001 acgaaccacg gcgtcgcgct ccatgcaaac gcgccgatgg caagcccgat catgccgaac
     3061 acgatgagca cggcatccca accgcgtgcg tcctccggtg ccagccccgc cacctcccgg
     3121 ttcggtgcgc gtgcggcaag ggagaccgag ccgcgcaggc cgctgcagcg cgagcacatg
     3181 tggcactccg agggaccggt catccgcttg atgcgaacca tcggggcgca attcaccgga
     3241 tgcacgggaa tgtgctcggc attgaccgcc cagccggcgg cgtcgacgcg gtagtggacc
     3301 ggggcaaggc gcgaaaggac accgaacacg ccgttgaccg ggcacaggtg gcggcaccag
     3361 acgcgcttcc cgcgcccata gacgaagccg accgcgatgg cggcgagcgt cgatccgccg
     3421 agcacgacca gcgtcgccgt cgggtactga tacacgctga cgagctggcc gtagaccgtc
     3481 gtcagcgcga acgcgacgaa cggccagccg ccccagcgca tccagcgcgg cacggcgcga
     3541 cccaggccat ggcggctcgc cgcctcggtc agcgtccctt cggggcagaa cacgccgcac
     3601 caggtgcgtc cgagcacgag catcgaaacg atcacgagcg gccaccagag gccccagaac
     3661 agccactgcg ccgcgaccac cacgctgttg taccaatgcg cgtcgtccgg tggaagcggg
     3721 agcaacgcag gcgtgatgac cagcagtgcg tacacgccca cgacgctcca ctggatgccc
     3781 tgcagcacgc gcgcatgcag gcgcatccat tcgccgaatg ctgcgaggcg cgccgccgca
     3841 cgtgcaggca aggtggcgga cgcggcgcta cttcgcgacg atgcggccct tgccggtatc
     3901 agggtggaac tcatcgaaaa aggtgtaact gccgggcttg aggccgttga tgacgaccga
     3961 cgagcgcgcg ccggccgcca gcactttttc ctggcgcaga tcgcggctct cgaactcgat
     4021 cggcccgctg cccgcgttga cgatctcgat cttgaagcgc gtgccggccg gcacctcgat
     4081 cgtcgccggc gcgaatacac cgtcgcgcgc cgacagcgag tacacccgca tcggctcggc
     4141 ccccgcagcg acacccgccg cgagcaaggc ggcggcgagc agcattgccc cgcgcgcgtg
     4201 ccgccgtgcg atcgtgcgtc gcctggccat gcgacgcatc ctcagtagcc gcccgtcttc
     4261 ccgaggcccg ggtacgtgaa ttcgtagtcg agcgagaatg gcttgaacca cggcgcaacg
     4321 ccggtttccc tgtccgtatg ccggccgaag tgcgaattcg cgcccggtgg cgcaatcgtc
     4381 agcgtcagcc gatacctgcc cgcgccctgg agcttcacgt tgtcgccgta atgcggcccg
     4441 tcgttggcaa ccatcggcat cagcgcgccc tccagcggcg gtgcattccc ctgcttcacg
     4501 atgcggtagc gcacgtcgag gtacggcatc cacgcgccgt ccgcgaagcc gttcgcgttg
     4561 tcggcttgcg cgcggatgtc ggcttcgaga tggatgtccg agtcgcggac cgcgcgcatc
     4621 atcgcggcgg gctccatcgg caccggttgc agatagacgg cgctcacctc gatgcccgcg
     4681 cggatctgcg gcttgccgat cgggtactcg agcgcgctcg ccggcgatgc agcgagcagg
     4741 cagatcgcga acacgaccga cgggttcgaa gtgatgaatc ggcggatcgg catgggtggc
     4801 ttcccgcggc gactcgaacg cccgccgcga atgaacggaa agggttccga gcttaccggg
     4861 cacgctcgcc gatgtaaata ggagtgattt gcatttgcat ctgactcggg ctagaatcgg
     4921 ccgtcgcacc gatccctgtt gcgttcgcgc cgaccgccat gtacatctgc ctctgcaatg
     4981 ccatcaccga tcgccagatc gtcgaggccg cccacggcgg cgcgcgcacg gacgaggatc
     5041 tcgcccaggg cctcggcgtg ggaatcacgt gcggtcgctg ccggacctgc gcgaaggagc
     5101 tgctgatgga aacactggcg cgcatcgctc ccgatgcctc cgactcgcgg accgcgcgcg
     5161 atggagcggc cgggtgaagg gcgacccgca gatcgtcaag cggctgaacg aggtgctcac
     5221 caacgagctg accgcgatcg accagtactt cctgcacgcg aaaatgttct cccattgggg
     5281 ccttgcgcat cttgcggcac gcgagcgcca tgcatcgatc gacgagatga agcacgccga
     5341 gtcgctgatc gagcgcatcc tgtttctcga ggggctcccg aacctgcagg acctgcacaa
     5401 gctgctgatc ggcgaaaacg tccgggaagc cctcgagtgc gacctgcggc tcgagcttgc
     5461 cgcgcatcca acgttgcgcg cggccatcgc cgactccgag gccgcggccg attacgtgag
     5521 ccgcgacctt ttccgctcga tcctcgtctc cgaggaagag cacatcgact ggctggaaac
     5581 gcaactcgac ctcctcgaca aggtcgggct cgccaattat cagcagtcgc agatcgacgc
     5641 cggcgactga cgccggacgc tccatatcct tcccggctat gaatacatag cccttcattc
     5701 cttccggttg cagcgcagcg cccgcatcat tggcgcatgc gtcccatcga gcaactggaa
     5761 agcgtgcgcg cgttcgacga cccggcggct tcgcgcgccg tgcccgcaac gctcctgtcc
     5821 gaggaacagc gtcacgcgct cgaccgcttc gtcgagccgc tgtcgccgga gcagctcatc
     5881 tgggtcggcg gttatctcgc agggctcgcg gcggcccatc gtacgacggc gcaccggccg
     5941 cccgccgacg cggccgaacg cgacgaatcg atcgtcacgg tcgtgtacgg ctcgcagtcg
     6001 ggcaacggcg cgcgcgtcgt cgcggaggtg aaggcgcagg ccgaggcgcg cggactcaag
     6061 gtgcgcgtca agccgatgga cgaataccgg ccgagcgatc ttcgggccga gaagaagctg
     6121 ctggtcgtcg tcagcaccca cggcgaaggc gagccgcccg acaacggcaa ggaattccac
     6181 gaattcctgc acggcaggaa ggcgggacgg ctcaacggcc tccactacgc ggtcctggcg
     6241 ctcggcgatt cgagttacca gcatttctgc aagaccgggc gcgacttcga cgagcgcctt
     6301 caatcgctcg gcgccgaacg cattcacccg cgtgccgact gcgacgtcga ctacgacgac
     6361 gccgccgcgc gatggatcga cggcgtgctc gacgcgctcg aaagcgacgc gacgcgaagc
     6421 gagccgcaac ccgaccgccg cgtcgcgatc gcgcatgcca aggtcacccc gctccattcg
     6481 aaaaggaatc cgtttccggc aaccgccgtt gcgaatatca acctgagcgg ccacggctcg
     6541 ggcaaggaag tgcggcacct cgagctcgcg ctcgaggggt cggcgctcaa tcacgagccc
     6601 ggcgattcgg tgggtatcct gccgacgaac gaccccgcgg gcgtggccga gctcatcgag
     6661 gcgctcggtc tcgaggcgtc gagcgccgtc gacgccggca atgaaacgac aacgctgcaa
     6721 ttcgcgctcg ctcacctgta cgaaatcacg acgctcaccc ggccgctcgt cgaacggtat
     6781 gccgaactga gcgatgcgca ggtgctgcgt gcaatgctgg ccgacgagcg cagcgacgcg
     6841 ctgtggactt atctgcgcgg acgccacctg atcgatctcg tcagcgagtt tccggttcgc
     6901 gggctcgctg cgcccgatct gctcggcatg ctgcgcaggc tgccgccgcg gctgtactcg
     6961 atcgcgtcga gcctcaaggc caatcccgga gaaatccatg caacggtggc cgcggtgcgc
     7021 tacgaaagcc acggccgcca gcgccgcggc gtcgcgtcga cgttcgttgc ggagcgcatc
     7081 gccgaaggcg accaggttcc gatctacgtc gaagccaacc ggaacttcaa gcttcccgcc
     7141 gatcccgcaa cgccaatcat catggtcgga cccggcaccg gtgtcgcgcc gttccgcgcc
     7201 ttcgtgcagg agcgtgaagc aatcggcgca accggcagga actggctgtt ctacggcgac
     7261 cagcgcttca ccaccgactt cctctaccag cgcgaatggc agcagcatct gaaggacggc
     7321 gtgctcacgc gcatggacgt cgcattttcg cgcgaccgga cgccgaagac gtacgtgcag
     7381 caccggatga aggcgcaggc gcgcgagctg tacgcgtggc tcgaagacgg cgcgcatatc
     7441 tatgtctgcg gcgatgccga gcgcatggcg cgcgacgtgc accagacgct gatcgagatc
     7501 gtcgccgagc acggacacct gtcgctcgac cgcgcgacgg agcacgtgca ggaactgcag
     7561 cgcacgcgcc gctatcagcg cgacgtgtat tgaccgacgt gcgcgttgcc gccgaaggac
     7621 gcgaagcgtg aaggaggatg ccgaagccgc cggccgctcg gccgtcgagc acatcaagga
     7681 cgccagcgat tatctgcgcg gctcgatcgt cgcaagcctc gccaatccgg tcaccggcgc
     7741 gcttgccgac agcgacacgc agctcatcaa attccacggc agctatcagc aggacgaccg
     7801 cgatctgcgt gacgagcggc ggcgcaaaaa gctcgagccg ctttactcgt tcatggtgcg
     7861 cgtgcgcgcg ccgggcggcg tcgtcacgcc ggcgcagtgg ctggtgctcg acgagctcgc
     7921 gcgcacacat gcgaacgggt cgctacgcct caccacgcgc cagtcgttcc aggtccacgg
     7981 tatcggcaag ccgcagctca aggacaccat cgcctcggtg aacaggacgc tgctgacgac
     8041 cattgccgcg tgcggcgacg tgaatcgcaa cgtcatgtgc aatcccaatc cgtatcagtc
     8101 ggcgctgcat cgcgaggtgt tcgactgggc gtgtcgcatc agcgaggatc tgacgccgca
     8161 cacgcgtgcg taccacgaaa tctggctcga cgatcggcta attgcgggcg gcgagcccga
     8221 tcacgagccg atctacggcc gcacgtacct tccgcgcaaa ttcaagattg ccattgcggt
     8281 gccgccgagc aacgatgtcg atgtgctcgc gcacgacctc ggcttcatcg cagtcgtcga
     8341 cgacggccgg ctcgcgggct tcaacgtcag cgtcggtggc ggcatgggga tgacgcacgg
     8401 tgagccggcg acctatcctc gtctcgccga cgtgatcggc ttctgcacgc cggatcgggt
     8461 gctggccgtc accgagcagg tggtcgccgt ccagcgtgat ttcggcgatc gcagcgaccg
     8521 caagcacgcg cggctcaagt acacgatcga cgaccgcggc atcggctggt tcaccgccga
     8581 gctcgcgtcg cgcctcggcc atcggctcga agccccacgg ccgtttcatt tcgagaccaa
     8641 cggcgatcgc tatggatgga ccgaagggat cgacggcaag ctgcatctga cgctcttcat
     8701 cgagaacggc cgcgtgcacg acacggcaga atgcctgctg atgacgggac tgcgcgaaat
     8761 cgcgaagatc cacgatggcg actttcggct gacggcgaac cagaacctga tcgtcgcgag
     8821 catcgatccg gcgaaacgtc cgcagatcga atcactgctt gcgcgccatt gccttgccga
     8881 cgccgaccac cgctcggcgc tgcggctgaa ttcgatggct tgcgttgcac tgccgacctg
     8941 cggtctcgcg atggcggaga gcgagcgtta cctgccgagc ctgatcggca agctcgaacg
     9001 cgtcctcgac gagtgtggtc tcgcgaacga cgcaatcacc gtgcgcatga ccggctgccc
     9061 gaacggctgc gcgcggccct atctggccga gattgccttc gtcggcaagt cactcgggaa
     9121 atacaacgtg tacctgggcg gcggatttgc gggcgagcgg ctgaacaagc tctatcgcag
     9181 tgcgctctcc gaagcggaaa ttctcgccga gctcacgccg atcctgcagc gctacgcggc
     9241 cgaacgcagg cagggcgagc gcttcggcga ctttgcgatt cgcgcaggct acgtgaacga
     9301 ggtcagggcc ggacgcgagt tccacgcctg agcgcaacgc ccgggcgccc acggaaggct
     9361 tcgcgccgtc acgcgtgcgg cgttgcagac gcctcgggca gccgcaggag gctgcgcgct
     9421 tcggcgatgg ttgccggccg gcggccgtat tcgccgcaca gttgcgccac ttggcgcacg
     9481 agcgccgcat tcgacggcgc gagcgtgtcg cgattcatgc gcacgttgtc ctcgagccct
     9541 gtgcggcagt gaccgccgag ctcgagcgac cagcgcacga gcgtcacctg gtccttgccg
     9601 atgcccgcac cggtccatgt ggcatcgggc tcgagacgct gcagcgtgcg cacgtaaaag
     9661 tcgaacgcgt cgcggtcgac gggcatcgcg tttttcacgc ccatcacgaa ctgcacatgg
     9721 agcgcgccct tgatccggcc ctcgcggcgc aaggcggacg ccttgaagat catcgacagg
     9781 tcgaacgcct cgacctcggg cttgatgccg tagcgcacca tctcgccggc gagccagtcg
     9841 acgaggtccg gtggattttc gtaaacgcgc gtcgggaaat tgcacgagcc ggtcgcgagc
     9901 gaggccatat cggggcgcag tgacagcatg gcgccgcgct cctttccggc gcccgagcga
     9961 ccgccggtcg agatttgggt gatcatgccg ggacagtgcc ggcgaatgcc gtcgacgacc
    10021 acggcaaagc gctcgggctc ggacgtgggc gattcgtcgt ccttgcgcac gtgaatgtgc
    10081 acgagtgcag cacccgcctc gaatgcctcg tgcgtggatt cgatctgctc ggccgccgag
    10141 atcggtacgg ccgggttatc cttcttgcgt ggcagcgagc cggtgatggc tacggtgatg
    10201 atgcacggcg ttgttgccat ggcgcctcca ttcgatgcgt atggcgatta tcgcgccttc
    10261 gcgtgacgct atagtagcca tgatgtcaca accgtgaccc gcgtcaccgc ccgttgcgat
    10321 cgcctgcgca ctccccttca cacatgattt ccggcaagac cacgctgatt gcgcatctcg
    10381 gttatccgac cgacgcgttc aaggcgccca tgatctacaa cccctggttc gagcatgcgg
    10441 ggatcgacgc cgtcgtcgtc ccgatgggcg tgcgcgccga ggattacgag gcgttcctcc
    10501 cgcagttgtt ccgcttgacg aacatccggg gagcgctcgt gacgatgccg cacaaggtga
    10561 cgaccgtcgg cctgctcgac gaagtcacgt cgacggtgct cattgccggc tcatgcaacg
    10621 cgatcgtgcg ccgggccgat ggctcgctgc tcggcgacat gttcgacggc gagggattcg
    10681 tgcgcggcgt gcgccgcaag ggccgcacga tcgcgggcgc acgtgcgctc gtcgtcggca
    10741 gcggaggtgt cggttcggcg attgcagcct cgctcgcggc ggcaggcgtt ggcgagctcg
    10801 gcctcttcga cgtcgacgcg gccgtcgcgg tcgcgctcga aagccggctg acgcggcacc
    10861 acccgcggct gcgtgtggtc accggctcga acgatcccga cggctgcgac atcgtcgtca
    10921 acgcgacgcc gctcggcatg aagcgcgacg atcccctgcc gctggacacg cgccggctgc
    10981 cgccgggcgc gttcgtcggg gaagtggtga tgacgcagga gacgacgccg tttcttcgcg
    11041 ccgcgctcga gcgcggatgc gaggtgcagg tgggaaccga catgctgttc gagcagattc
    11101 ccgcgtatct cgaattcttc ggattcggat ccacgacggc ggacgtgctg cgtgcagtgg
    11161 cgaagatcga gtactagcgg ctgcggccgc gttggctcga cgcgggcggc ccattggcca
    11221 cgccgcgcgc tcgcgcgaaa cccgggctcg ccttgccgat gatgatgctg ctcgcggtga
    11281 cggtattcgc gtccgcgagc accgtgcatt ttcagacacc gatgctcggg cgcttcggcg
    11341 tcgaattcgc ggcgacgcct gcggcgatcg gatggatacc aacgctgtcg ttcgcgggtt
    11401 ttctggccgg catcctgttc ctggttccgc tcggcgaccg catcga
//
LOCUS       DASSVX010000250         5403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_378031, whole genome shotgun sequence.
ACCESSION   DASSVX010000250 DASSVX010000000
VERSION     DASSVX010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378031"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2854
                     /locus_tag="VFO53_10760"
     CDS             <1..2854
                     /locus_tag="VFO53_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8876798.1"
                     /translation="FEPGTNVDQSIFEVNNRVQRALPRLPDEVRRNGVIVQKRSNDIL
                     LVVGLVSRSAAQKTTFMADYATVNMIDELKRVPGVGDVTLFGSGYAMRIWLQPDRMAR
                     LGVTPTDVAAAIRSQNQQYAAGKIGAEPAPAGQALVYTVTARGRLVTPEQFGNIVVRA
                     GGPSGVLRIRDIARVELGAQSYDISTTVDGQPAVGMAVFLQTGANALDVGAAVKARVR
                     ELKTAFPEGMDYIIPFDTTRVIDASIKEVLKTLGEAAVLVLLVVFVFLQHWRATLIPM
                     IAVPVSLIGTFAGLYALHFSINTLTLFAMVLAVGIVVDDAIVVLENVERLMREQKMGA
                     KDAAIASMHEVSGAVFAIVLVLCAVFVPVAFLGGIAGQLYRQFAVTVAISVLLSGLTA
                     LTLTPALCALLLKAGEHESRLFHPFNVGFAKLTAAFLWMIGIAIRRRLLAGVAFLGVL
                     ALVGLLFLHVPGSFVPSEDQGYLFGAIMLPDAATLQRTDAAGAQLQRGLAADPAIEHI
                     FVAPGRDLIGGGNKPNSGTTFLLLKDWENRDKTALAIASEMSRLGQTLPDGVAIGFNP
                     PPIRGLGTAGGFEAYVQARVDSSAQRLADVVQAYTAALARDPRLTGINTFYRPSVPQV
                     YVEVNLEQAMALGVPVSDVFDALQSTIGALYVNDFNMSGRTYRVQVQADAPYRAQPDD
                     LGAVYVRSNTSGEMIPLKALVRTTSIVGPEQVERYNGFLAAKVLGSGKPGVSSGDAIK
                     AAEDVAASVLPAGYTLEWTGQAFQEKRTGRASLFAFGFAIIMVFLILAALYERWRLPA
                     AVVFAVPFAVLGALSLVWLRGMENDIYFQIGLVVLIGLSAKNAILIVEFAQQGLLAGM
                     HPVDAALQAARLRFRPIVMTSIAFVFGVLPLTFASGAGAAARRSMGTGVVGGMLLATF
                     VATIFVPLFFTLFSRPVKASGPAPADAAATLAGKPA"
     gene            2854..4248
                     /locus_tag="VFO53_10765"
     CDS             2854..4248
                     /locus_tag="VFO53_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET8876799.1"
                     /translation="MRRIAVACAVAGVVACAPVGPDYRRPDLDVPKAYAESPSAEAAL
                     PAVPADWWRLYQDATLDRLVGAGLTQGTDVRLAIARIEEAAALRREANATLLPEIDAS
                     GVVSRSGASTQTPSVRAGAASTLGNFALSANTAFELDFWGRLRRLREAAQAQYTATDY
                     ARGVVELSLTAAIARTYFNVRSLDAQIAVSAESLAAAEDSVAITRRRSEGGIASELDV
                     YQAQGNRAQLAAQIEELRRQRAVSMHQLGVLSGEPALDLPPADLRAIPTPPLPPAGLP
                     SALLERRPDLRQAEASLVAANAQIGVARAAQLPTISLTASLGLQSDNLAHILSPGADI
                     WSVGVGLLGPVIDWGRYAARTEEAEARARQSQILYEQALRTAFREVSDALSNVGLAVQ
                     TEQDLTARVDYANNSLRLATQRYEAGYSAYLEVLDAQRTLNDAQLALVRNRQSLLTFT
                     VDLMNALGGGWTAA"
     gene            complement(4323..4751)
                     /locus_tag="VFO53_10770"
     CDS             complement(4323..4751)
                     /locus_tag="VFO53_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET8876800.1"
                     /translation="MKRPPCNLGFLVADISRLMRRAYRQRQQRSALTVAQARTLVHVA
                     RREGVRQVDLAETLEVQPITLARLIDQLARAGLVERRPDPTDRRAYRVHLTRAAVPHL
                     SAIEEVAASIRADALRGLDAREATLVIDALQKIHANLAGG"
     gene            complement(4833..>5403)
                     /locus_tag="VFO53_10775"
     CDS             complement(4833..>5403)
                     /locus_tag="VFO53_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HET8876801.1"
                     /translation="AKVKRNNQVDEHSALARKAGDVVVAALERNALFISAALPHRVYP
                     PLFNRYEGGMHFGEHVDGAVRLVPRTGAKLRTDLAATLFISDSAEYDGGELVIDDTYG
                     AHAAKLPAGHMIVYPADSRHRVTAVTRGARVASVLWVQSLVRDVTRRRLLFDLDTAVQ
                     RLTAGGADDPARLQLAGCYHNLLRMWAET"
BASE COUNT          778 a         1849 c         1880 g          896 t
ORIGIN      
        1 cttcgagccg ggcaccaacg tcgaccagtc gatcttcgag gtgaacaacc gcgtacagcg
       61 cgcgctgccg cgcctgcccg acgaggtgcg ccgcaacggc gtcatcgtgc agaagcgatc
      121 caacgacatc ctgctcgtcg tcgggctcgt gtcgcgttcg gctgcgcaga agacgacgtt
      181 catggccgac tacgcgacgg tcaacatgat cgacgagctg aagcgcgtgc ccggcgtcgg
      241 cgacgtgacg ctgttcggat cggggtacgc aatgcgcatc tggctgcagc ccgaccggat
      301 ggcgcgcctc ggtgtgacgc cgaccgacgt cgccgcggcg attcgctcgc agaaccagca
      361 atacgccgcg ggcaagatcg gcgccgagcc ggcgcccgcg ggccaggcgc tggtctatac
      421 ggtgaccgcg cgagggcgat tggtcacgcc ggagcagttc ggcaatatcg tcgttcgcgc
      481 cgggggtccc tccggcgtgc tgcgcattcg cgacatcgcg cgcgtcgagc tcggcgcgca
      541 aagctacgac atcagcacca ccgtcgatgg ccagcccgcg gtcggcatgg cggtgttcct
      601 gcagacgggc gccaatgcgc tcgacgtcgg tgcggcggtc aaggcgcgcg tgcgggaatt
      661 gaaaacggcg ttccccgaag ggatggacta catcatcccg ttcgacacga cgcgcgtcat
      721 cgacgcgtcg atcaaggaag tgctgaagac gctcggcgag gccgcggtgc tcgtgctgct
      781 cgtcgtgttc gtgttcctgc agcactggcg cgcgacgctc atcccgatga tcgcggtgcc
      841 cgtttcgctg atcgggacgt tcgccggcct gtacgcgctg catttctcga tcaacacgct
      901 gacgctgttc gcgatggtgc tcgcggtcgg catcgtcgtc gacgacgcga tcgtcgtgct
      961 cgagaacgtc gagcggctga tgcgcgagca gaagatgggc gcgaaggacg cggcgatcgc
     1021 gtccatgcac gaggtgtcgg gcgccgtgtt cgcgatcgtc ctcgttctgt gcgcggtgtt
     1081 cgtgcccgtc gcgttcctcg gcggcatcgc cggccagctc tatcggcagt tcgcggtgac
     1141 ggtggcgatc tcggtgctgc tttcgggact gaccgcgctc acactgacac ccgcgctttg
     1201 cgcattgctg ctgaaggcgg gcgagcacga gtcgcgcctg tttcatccgt tcaacgtcgg
     1261 gttcgcgaag ctcacggcgg cgttcctgtg gatgatcggc atcgccatca ggcgccggct
     1321 gttggccggc gtcgcgttcc tcggtgtgct cgcgctggtc gggctgctgt tcctgcacgt
     1381 gccgggaagc ttcgtgccgt ccgaggatca ggggtacctg ttcggcgcga tcatgctgcc
     1441 cgacgccgcc acgcttcagc gcaccgacgc cgccggcgcg caattgcagc gcggccttgc
     1501 ggccgatcct gcgatcgagc acatcttcgt cgcgcccggc cgcgacctga tcggcggcgg
     1561 caacaagccg aattcgggca cgacgttcct gctgctcaag gattgggaga accgcgacaa
     1621 gacggcgctc gcgatcgcaa gcgagatgtc acgcctcgga cagacgctgc cggacggcgt
     1681 cgccatcggc ttcaacccgc cgccgattcg cggtctcggc accgcgggcg ggttcgaagc
     1741 gtacgtgcag gcgcgcgtcg attcgagtgc gcagcggctt gccgacgtcg tgcaggcata
     1801 caccgccgcg ctcgcgcgcg atccgcggct tacgggaatc aacacgttct atcgtccgag
     1861 cgtgccgcag gtctacgtcg aggtgaacct cgagcaggcg atggcgctcg gcgtgccggt
     1921 gtcggacgtg ttcgacgcgt tgcagagcac gatcggcgcg ctctacgtca acgacttcaa
     1981 catgtcgggg cgcacgtatc gcgtgcaggt acaggccgac gcgccgtatc gcgcgcagcc
     2041 ggacgatctt ggcgccgtgt acgtgcgctc gaacacgagc ggcgagatga ttccgctgaa
     2101 ggcactcgtg cgcacgacga gcatcgtcgg ccccgagcag gtggagcgct acaacggctt
     2161 cctcgcggcg aaggtgctcg gcagcggcaa gccgggggtg agttccggcg acgcgatcaa
     2221 ggccgccgaa gacgtcgccg ccagcgtgct gcctgcgggc tacacgctcg agtggaccgg
     2281 ccaggccttc caggagaagc gcaccgggcg tgcgtcgctt ttcgcgttcg gcttcgccat
     2341 catcatggtg ttcctgatcc tcgccgcgct gtacgagcgc tggcgtctgc cggccgccgt
     2401 ggtgttcgcg gtgccattcg cggtgctcgg cgcgttgtcg ctcgtgtggt tgcgcggcat
     2461 ggagaacgac atctatttcc agatcggcct cgtcgtgctg atcgggctgt cggcaaagaa
     2521 cgcgattttg atcgttgaat tcgcgcagca gggcctgctt gccggcatgc acccggtcga
     2581 tgcggcgttg caggccgcgc gcctgcgctt ccggccgatc gtgatgacgt cgatcgcatt
     2641 cgtgttcggc gtgcttccgc tcacgttcgc gtcgggcgca ggcgctgcgg cgcgccgatc
     2701 gatgggaacc ggcgtggtcg gcggcatgct gctcgcaacg ttcgtcgcga cgatcttcgt
     2761 gccgttgttc ttcacgctgt tctcgcgtcc ggtgaaggcc agcggccctg ccccggccga
     2821 cgccgcggcg acactcgcgg gcaagcccgc gtgatgcgcc ggatcgcggt tgcgtgcgcg
     2881 gtggcgggtg tggtcgcctg cgcgccggtg gggcccgact atcgccggcc cgatctcgac
     2941 gtgccgaagg cgtacgccga aagcccgtcg gcggaagccg cgctgcccgc cgtcccggcc
     3001 gactggtggc gcctgtacca ggatgcgacg ctcgatcgcc tggtcggcgc gggactcacg
     3061 caggggaccg acgtgcgcct tgccatcgcg cgcatcgagg aagccgcggc gctgcgccgc
     3121 gaagcgaatg caacgctgct gccggagatc gacgcgtcgg gcgtcgtatc gcgctcgggt
     3181 gccagcacgc agacgccttc ggtgcgcgcg ggtgcagcgt cgacactcgg caacttcgcc
     3241 ctgtccgcca acaccgcgtt cgagctcgac ttctggggac gcctgcggcg cctgcgcgag
     3301 gccgcacagg cgcagtacac ggccaccgac tatgcgcgcg gcgtcgtcga gctctcgctc
     3361 accgctgcca tcgcgcgaac ctatttcaac gtgcgctcgc tcgatgcgca gatcgcggtg
     3421 tcggcggaaa gcctcgccgc ggccgaggat tcggtcgcga tcacgcgccg gcggtccgaa
     3481 ggcggcatcg cgtccgagct cgacgtgtac caggcgcagg gcaaccgtgc gcagctcgcg
     3541 gcacagatcg aggagctgcg ccgccagcgc gccgtctcga tgcaccagct cggcgtcctt
     3601 tccggtgaac cggcactcga cctgccgccg gccgacctgc gcgccatccc gacgccgccg
     3661 ttgccgcccg cggggctccc gtcggcgctg ctcgagcggc ggcccgatct gcgccaggcg
     3721 gaagcatcgc tcgtcgcggc caacgcgcag atcggcgtcg cgcgcgccgc acagctgccc
     3781 acgatcagcc tcaccgcgtc gctcggcctg cagagcgaca acctcgcgca cattctttcg
     3841 cccggtgcgg acatctggtc ggtcggcgtc ggattgctcg ggcccgtgat cgactgggga
     3901 cgctacgccg cgcgtacgga agaggccgag gcgcgcgcgc gacagtcgca gatcctctac
     3961 gagcaggcgt tgcgaaccgc ctttcgcgaa gtgtccgatg cgctgtccaa tgtcggactc
     4021 gccgtgcaga ccgagcagga cctcaccgcg cgggtcgact acgccaacaa cagcctgcgt
     4081 ctcgcgacgc agcgctacga ggccggctat tcggcgtacc tcgaggtgct cgacgcgcag
     4141 cgcacgctga acgacgcgca actcgcgctc gtgcgcaatc gtcaatcgct gctcacgttc
     4201 accgtcgatc tgatgaacgc gctgggtggc ggctggaccg cggcctgacg gcgcacgcac
     4261 caccgtcgac agccgttgcg cgccgccgcc cggcgcgagc gaggccgtcg cgcttgccaa
     4321 gctcagccgc cggcgaggtt cgcgtgaatc ttctgcagcg cgtcgatgac gagcgttgcc
     4381 tcgcgtgcgt cgagcccgcg caacgcatcg gcgcggatcg acgccgccac ctcctcgatt
     4441 gccgacagat gcggcaccgc cgcgcgcgtg agatggacgc ggtatgcgcg ccggtcggtc
     4501 ggatcgggac gccgctcgac gagtcccgcg cgcgcaagct ggtcgatcag gcgcgcgagc
     4561 gtgatcggct gcacctcgag cgtttcggcg agatcgacct gacgcacgcc ctcgcgccgc
     4621 gcgacgtgca cgagcgtgcg tgcctgcgcc acggtcagcg cgctgcgctg ctgtcgctgc
     4681 ctgtacgcgc ggcgcatgag gcgcgaaatg tcggcgacga ggaatccgag gttgcagggc
     4741 ggccgcttca tccgctgctc atataataag gattgctcat tatattcgcg tgcggggcag
     4801 ccgctaggcg tgccggtctc ggcgcgtgcc cgtcatgtct cggcccacat gcgcagcaga
     4861 ttgtggtagc agccggcaag ctgcaaccgc gcaggatcgt ccgcgccgcc cgcggtgagc
     4921 cgctggaccg ccgtgtcgag atcgaacagc agccgccgcc gggtgacgtc acgaacgagg
     4981 ctttgcaccc acagcacgct cgcgactcga gcgccgcgcg tgaccgcggt gacgcggtgc
     5041 cggctgtccg cgggatagac gatcatgtgt ccggcgggca gcttcgccgc gtgcgcgccg
     5101 tacgtatcgt cgatcaccag ctcgccgccg tcgtactcgg cactgtcgct gatgaacagc
     5161 gtcgccgcga gatcggtgcg aagcttggca cccgttcggg gaacgagccg cacggcgccg
     5221 tcgacgtgct cgccgaagtg catgccgcct tcgtagcgat tgaagagcgg cgggtacacg
     5281 cggtgcggca acgcggcgct gatgaaaagc gcgttgcgct cgagcgccgc cacgacgacg
     5341 tcgccagcct tccgcgcaag cgccgaatgc tcgtcgacct ggttgttgcg ctttaccttg
     5401 gcc
//
LOCUS       DASSVX010000251         7010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_211407, whole genome shotgun sequence.
ACCESSION   DASSVX010000251 DASSVX010000000
VERSION     DASSVX010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211407"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..935
                     /gene="ispD"
                     /locus_tag="VFO53_10780"
     CDS             204..935
                     /gene="ispD"
                     /locus_tag="VFO53_10780"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET8876802.1"
                     /translation="MSVRTWIIVPAAGGGVRFGAATPKQYADLAGRPVLLRTLERLAA
                     LDAAAIVVALAPDDVRFDELIGARPDVLPLRCGGATRGATVRRALQAIAPRCGDGDWI
                     LVHDAARPCVPRESLAHLVQELADDAVGGLLALPLADTLKRAAATGSPPRVMRTEERE
                     GLWLAQTPQMFRHGLLVAAHRLDVAGRCTDEAQAIEALAATGVCAMPKLVRGSPANVK
                     ITHPGDLALAAAILRMQGAHASGAT"
     gene            969..1460
                     /gene="ispF"
                     /locus_tag="VFO53_10785"
     CDS             969..1460
                     /gene="ispF"
                     /locus_tag="VFO53_10785"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET8876803.1"
                     /translation="MRFGNGFDVHAFAEGRPLVIGGVSVPHDRGLAGHSDADVLLHAI
                     TDAILGALALGDLGRHFPDSDARWKGIDSRRMLRDVAAMMSAARYTIGNVDATVIAQS
                     PRLAPFVDAMRMNIAHDLGCDLSQVSVKATTTERLGFAGREEGMAALATVLLAAMENQ
                     SGS"
     gene            complement(1518..2543)
                     /locus_tag="VFO53_10790"
     CDS             complement(1518..2543)
                     /locus_tag="VFO53_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876804.1"
                     /translation="MSLKPFARRASLWCSVAALALANLSANALGQEIKVTRQYGLPYL
                     PLMVMEHEHLFEKHLKALGAGDAKVTWATMSTTTATVDGLLSGSVDFASSGITGLLAM
                     WDKTQGTRNEIRAISGISSFPYPLVTSNAAVKTLKDFTDKDRIAVPAVKISVQAIVLQ
                     MAAAQAFGADKRTALDAFTVSMGHPTAAVAVMSGKSEVNAHFSYPPYSQEELRTPGVH
                     AVLWANDVTHGPATTIEVVTPGRVMNEHPKWVDAWLAAQREASDFINQHHPEAAQMYI
                     AMTNEKKLTAGDLARILADPQVRISVTPYNTMVFAKYMHDVDSIKHLPASWKDYFWPV
                     AQGLEGS"
     gene            complement(2578..3783)
                     /gene="argE"
                     /locus_tag="VFO53_10795"
     CDS             complement(2578..3783)
                     /gene="argE"
                     /locus_tag="VFO53_10795"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HET8876805.1"
                     /translation="MQTASEADSAACAPPHSLELIRQLVQFDTTSRDSNLALIDWVCQ
                     YLASHGLAWRLTRDDTGAKANLFCTIGDPRRPGIVLSGHTDVVPVDGQAWSVDPWAAV
                     VTGERLVGRGTADMKSFLGIVLAAVPKMLARPLATPIHLAFSYDEEVGCIGVGRMILD
                     MQSIGVAPGICIVGEPTGMQVVTGHKGKLALRAHVCGREAHSALAPYAVNALEYAAEI
                     IVELRRMQRQAAQTGPFSREYDPPYSTLHAGVLHAGTALNIVPRECTLDFEFRTVPGD
                     DPHRLLTRLRAYCASELVPQMQRVAPESRIEFETRSDNPGLHTEETAQIVGRVERLAR
                     GGRGGKVAFASEAGQFQRAGFDAVICGPGFITDAHKPDEFITFDQIRQGEAFIARVVD
                     EAQQGVSAG"
     gene            complement(3891..5213)
                     /locus_tag="VFO53_10800"
     CDS             complement(3891..5213)
                     /locus_tag="VFO53_10800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8876806.1"
                     /translation="MSVVPRSLFGRLILLLVTVVALVAVTTIFAFKRDREALLAQQFN
                     DTKVVQMKALRASLDAVDGPARREALARLGREYGVRIVAERDRPTVGVVPIGSALTEL
                     EQRLRNDLGEGTEVRVSPRARQLFVRVQAGDDGYWIGFPLPPRAQSQDIPTRALAWAI
                     AIIVLLLLAAFAFARYLARPLGELRDAVERVGRGETPAPLPESGTSEIAALNRGFNAM
                     IGNLRQVERDRAILLAGVSHDLRTPLARLRLGIELSPADEATRAGMVGDIEEMDRIID
                     QFLDFARDDAATATEPIDVNAELATSVDRYVRAGSDVRIVRGAPAWARIKPTALSRVA
                     SNLIDNALAYGRPPLDVTAVRDGDDVLVDVADRGPGIAPGDVERLKKPFTRAGEARTR
                     TDGAAGAGLGLAIVDRIARLHGGRFDLRPRDGGGTLARLRLPAADRPS"
     gene            complement(5210..5962)
                     /gene="ompR"
                     /locus_tag="VFO53_10805"
     CDS             complement(5210..5962)
                     /gene="ompR"
                     /locus_tag="VFO53_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HET8876807.1"
                     /translation="MPKATSPKILVVDDDVRLRDLLSRYLSEQGFEVASMPDTSQLDR
                     RLQRDPPHLLVLDLMLPGEDGLAACRRLRGAGETVPIIMLTAKGEDVDRIVGLEMGAD
                     DYLPKPFNPRELVARIHAVLRRHGERPTPGAPTGEGRVPFGGHTLDLAARTLERDGEV
                     KALTTGEFSLINVFARHPRQPLAREKLMLLARGRDHEIFDRAIDVQVSRLRKLIERDP
                     ATPRYIQTVWGFGYVYVPEGTPPDDAAGDETK"
     gene            6169..6657
                     /locus_tag="VFO53_10810"
     CDS             6169..6657
                     /locus_tag="VFO53_10810"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HET8876808.1"
                     /translation="MTKRSASRLAAVAAATLIGLTGAAFAKPFHHGHGAGGDVVMSIM
                     RLKSQLNLNTSQQTMWDNAVAAGKAARVTARTNMQKVHDTLASELAKPEPDLAAVSAA
                     SDSARTANAALHAQVRDAWLNVYSTFTPEQKGVVKSALSNRMAKMEQFREKMLQRHSN
                     GG"
     gene            complement(6787..>7010)
                     /locus_tag="VFO53_10815"
     CDS             complement(6787..>7010)
                     /locus_tag="VFO53_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HET8876809.1"
                     /translation="ANNRSGIDAVRQLRHELGVAIPAIIVSGDTGTGADTEARAAALM
                     LLPKPVVAATLHATAIALMAQRPVAMLPA"
BASE COUNT         1087 a         2358 c         2445 g         1120 t
ORIGIN      
        1 tgtaacgggg gaatcggtag tgaacatgcg tgacctggcg ccgctatccg gcgcgagcgc
       61 aaggcggcga tgcgcagcaa gggtaggccc ccttgcaagc agcgccaacg cagcgatcgc
      121 gccggagagc aagccaggca ctgccgattc ccccgttaca ggtcactagg gcggttggcg
      181 cggatcgcga gcaggcccga gccgtgtcgg tacgcacctg gatcatcgtt cccgccgcag
      241 ggggcggcgt gcgcttcggt gcggcgacgc cgaagcaata cgccgatctc gcggggcggc
      301 cggttctttt gcgcacgctc gagcggcttg cggcgctcga cgctgccgcc atcgtcgtgg
      361 cactcgcccc cgacgacgtt cgcttcgacg agctgatcgg cgcgcgaccc gatgtccttc
      421 cactgcgctg cggcggcgcg acgcgcggcg cgacggtgcg cagggcgctg caggccatcg
      481 caccgcgctg cggcgacggc gactggatcc tcgtgcacga cgcagcgcgc ccgtgcgtgc
      541 cgcgcgaatc gctcgcacac ctggtgcagg agctggccga cgacgcggtc ggcggcttgc
      601 tcgcgcttcc gctcgccgac acgctgaagc gtgccgcggc gaccggcagt ccgccgcgcg
      661 tcatgcgcac cgaggagcgc gaaggcctgt ggctcgcgca gacgccgcag atgtttcgcc
      721 atggcctgct ggtcgcggcg caccggctcg atgtcgccgg tcgctgcacc gacgaggcac
      781 aggcgatcga agcacttgcc gcgaccggtg tgtgcgcgat gccgaagctc gtgcgcggca
      841 gccctgccaa tgtcaagatc acgcatccgg gcgatctcgc gcttgccgca gcgatcctgc
      901 ggatgcaggg tgcgcacgca agcggggcga cgtagcggcg aatgccgcaa acgcggaggg
      961 aaatgcctat gcgcttcggc aacgggttcg acgtgcacgc cttcgcggag gggcgtccgc
     1021 tcgtcatcgg cggcgtgagc gtgccgcacg accgcggact ggcaggtcac tccgatgccg
     1081 acgtgctgct gcacgccatc accgacgcga tcctcggtgc gctggcgctt ggcgatctcg
     1141 ggcgtcactt tcccgactcg gatgcgcgct ggaagggcat cgacagccgg cgcatgctgc
     1201 gcgatgttgc agcgatgatg tcggcggcgc gctatacgat cggcaatgtc gatgcgaccg
     1261 tcatcgcgca gtcgccgcgg ctcgcaccct tcgtcgacgc gatgcgcatg aacatcgcgc
     1321 acgacctcgg ctgtgacctg tcgcaggttt cggtgaaggc gacgacgacc gagcgcctgg
     1381 gcttcgccgg gcgcgaggaa ggcatggccg cactcgccac ggttctgctc gccgcgatgg
     1441 agaatcagag cggttcctga gcggcgcgtc gcgcgcagcg actgcgtgcg ttcggccgcc
     1501 aggacgctcc cggccgctca gctgccctcg agaccctgcg caacgggcca gaagtaatcc
     1561 ttccagcttg ccggcagatg cttgatgctg tcgacgtcgt gcatgtattt cgcgaacacc
     1621 atcgtgttgt acggggtgac gctgatgcgc acctgcgggt cggcgagaat cctggcgagg
     1681 tcgccggcgg tcagcttctt ctcgttggtc atcgcgatgt acatctgcgc cgcctcgggg
     1741 tgatgctggt tgatgaagtc gctcgcttcg cgctgcgcag cgagccacgc gtcgacccac
     1801 ttcggatgct cgttcatcac gcggccgggc gtcacgactt cgatcgtcgt cgcgggaccg
     1861 tgcgtgacgt cgttcgccca gagcaccgca tgcacgcccg gcgtgcgcaa ttcctcctgg
     1921 ctgtaaggcg ggtagctgaa atgcgcgttg acctcgctct tccccgacat gaccgccacc
     1981 gccgcggtcg ggtgtcccat cgacacggtg aacgcgtcga gcgcggtgcg cttgtcggcg
     2041 ccgaacgcct gcgcggccgc catctgcagc acgatcgcct gcaccgagat cttgacggcg
     2101 ggaacggcga tgcggtcctt gtcggtgaag tccttcagcg tcttcacagc cgcattgctg
     2161 gtcaccagcg gatagggaaa cgacgaaatg ccggagatcg ccctgatttc gttgcgcgtg
     2221 ccctgcgtct tgtcccacat cgcgagcagg ccggtgattc ccgaggacgc gaaatcgacg
     2281 ctgcccgaca ggagtccgtc caccgttgcg gtcgtcgtgc tcatcgtcgc ccacgtcacc
     2341 ttcgcgtcgc cggccccgag cgccttcaga tgcttctcga acagatgctc gtgctccatc
     2401 accatcagcg gcaggtacgg caggccgtac tgacgcgtga ccttgatctc ctgcccgagc
     2461 gcgttcgccg aaaggttcgc cagcgcgagt gctgcgacgc tgcaccagag cgacgcgcga
     2521 cgcgcgaatg gtttgagtga cacgggtccg atctcctttt cgatgggaaa gcgtgcgtca
     2581 gcccgcgctg acgccttgct gcgcttcgtc gacgacgcgc gcgatgaacg cttcgccctg
     2641 gcggatctgg tcgaacgtga tgaattcgtc gggcttgtgc gcgtcggtga tgaagccagg
     2701 accgcagatg accgcgtcga aaccggcgcg ctggaactgc ccggcctccg acgcgaacgc
     2761 gaccttgccg ccgcgtccac cgcgcgcgag gcgctcgacg cggccgacga tctgcgccgt
     2821 ctcttcggta tgcagtcccg gattgtcgga cctggtctcg aattcgatgc ggctctcggg
     2881 ggcgacgcgt tgcatctgcg gcacgagctc gcttgcgcag tacgcgcgca accgcgtgag
     2941 cagccgatgc ggatcgtcgc cgggcacggt gcgaaactcg aaatcgagcg tgcactcgcg
     3001 cggcacgatg ttgagcgcgg tgcccgcgtg gagcacgccc gcatgcagcg tcgagtaggg
     3061 cggatcgtac tcgcggctga acgggccggt ttgcgcggct tggcgctgca tccggcgcag
     3121 ctcgacgatg atctcggccg cgtattcgag cgcgttgacc gcgtagggtg cgagtgccga
     3181 gtgcgcctcg cggccgcaga cgtgcgcgcg cagcgcaagc ttgcccttgt gcccggtgac
     3241 gacctgcatt cccgtcggct cgccaacgat gcagatgccg ggcgcaacgc cgatcgactg
     3301 catgtcgaga atcatgcggc ccacgccgat gcagccaacc tcttcgtcgt acgagaaggc
     3361 gagatggatg ggtgttgcga gcggtcgtgc gagcatcttc ggcaccgccg cgagcacgat
     3421 gccgaggaac gacttcatgt cggcggtgcc gcgtccgacc agacgctcgc cggtcacgac
     3481 cgccgcccac gggtcgaccg accacgcctg tccgtcgacc ggaaccacgt cggtgtggcc
     3541 ggacagcacg atgccgggtc gacggggatc gccgatcgtg cagaacaggt tggccttcgc
     3601 gccggtgtcg tcgcgggtga gacgccacgc aagcccgtgt gacgcgaggt actggcacac
     3661 ccaatcgatc agcgccaggt tcgaatcacg gctcgtcgtg tcgaactgca cgagttgccg
     3721 aatgagctcg agactgtgcg gcggcgcaca cgcagcgctg tcggcctccg aagcggtttg
     3781 cacggtcgat tcccggggat cagctgagct catcgacccg gccggtggcg gctcgccgat
     3841 gcgaggaaac gataccacgc ctccagccgc tcctgccctt acctacgagg ctacgacggt
     3901 cgatcggcgg ccggcagccg cagccgcgcg agcgttccgc cgccgtcgcg gggccgcaga
     3961 tcgaagcgcc cgccgtgcag gcgtgcgatg cgatcgacga tcgcaaggcc gaggcccgca
     4021 ccggccgcgc cgtcggtgcg cgtgcgggct tcgccggcgc gcgtgaacgg ctttttgagc
     4081 cgttcgacgt cgcccggcgc aatgcccggt ccgcggtcgg ccacgtcgac cagcacgtcg
     4141 tcgccgtcgc gcaccgccgt cacgtcgagc ggcggacggc cgtaggcgag tgcgttgtcg
     4201 atgaggttgc tcgcgacgcg cgacagcgcg gtgggcttga tgcgcgccca cgcgggtgcg
     4261 ccacggacga tgcgcacgtc gctgcccgcg cgcacgtagc gatcgacgct cgtggcaagt
     4321 tcggcgttca cgtcgatcgg ctcggttgcc gtcgccgcgt cgtcgcgcgc gaagtcgagg
     4381 aactggtcga tgatccggtc catctcctcg atgtcgccga ccatgccggc acgcgtcgct
     4441 tcatcggcag gcgacagctc gatgccgagc cgcagccgtg caagcggggt gcgaaggtcg
     4501 tgcgatacgc cggcgagcag gattgcgcga tcgcgctcga cctggcgcag gttgccgatc
     4561 atcgcgttga acccgcggtt cagcgcggcg atttccgacg ttcccgattc gggcagcggc
     4621 gcgggcgttt cgccgcgtcc gacgcgctcg accgcgtcac ggagctcgcc gagcggccgg
     4681 gcgagatagc gggcgaatgc gaacgcagcg agcagcagca gcacgatgat cgcgatcgcc
     4741 cacgcaagtg cgcgcgtcgg gatgtcctgg gattgcgcgc gtggcggcag cgggaagccg
     4801 atccagtagc cgtcgtcgcc ggcctgaacg cggacgaaaa gctgacgcgc gcgcggcgag
     4861 acgcgcactt cggtgccttc gccgagatcg ttgcgcagcc gctgctcgag ctcggtcagc
     4921 gcgctgccga tcggaacgac acccaccgtc ggccggtcac gctcggcaac aatgcgcacg
     4981 ccatactcgc ggccgagccg cgccagcgcc tcgcggcgcg ccggaccgtc caccgcgtcg
     5041 agcgaggcgc gcagcgcctt catctgcacg accttggtgt cgttgaactg ctgcgcgagc
     5101 aacgcctcgc ggtcgcgctt gaacgcaaag atcgtcgtga ccgcgaccag cgcgacgacg
     5161 gtcaccagca gcaggatcag gcgcccgaac agcgatcgag gaacgacgct cacttcgtct
     5221 cgtccccggc cgcgtcgtcg ggcggcgtgc cttccggaac gtagacgtag ccgaagcccc
     5281 acaccgtctg gatgtagcgt ggtgtcgccg gatcgcgttc gatcagcttg cgcagtcgcg
     5341 acacctgcac gtcgatcgcg cgatcgaata tctcgtggtc gcgcccgcgc gccagcagca
     5401 tcagcttttc gcgtgcaagg ggttgccgcg ggtgccgggc gaacacgttg atcagcgaga
     5461 actcgccggt ggtgagcgcc ttcacctcgc cgtcgcgctc gagcgtgcgc gcggcgagat
     5521 cgagcgtgtg tccgccgaat ggaacgcgtc cttcgccggt cggcgcgccg ggggtgggcc
     5581 gctcgccatg gcggcgcagc accgcgtgta tgcgcgcgac gagctcgcgc ggattgaacg
     5641 gcttcggcag gtaatcgtcg gcacccatct cgagcccgac gatgcgatcg acgtcctcgc
     5701 ccttcgccgt cagcatgatg atcggcaccg tctccccggc gccacgcagg cgccggcagg
     5761 ccgcgagacc gtcctcgccc ggcagcatga ggtcgagcac gagcaggtgc ggcggatcgc
     5821 gctggagccg ccggtcgagc tgcgacgtgt cgggcatcga cgcgacctcg aagccctgct
     5881 cggagagata gcgggaaagg aggtcgcgca gccgcacgtc gtcgtcgacg acgagaattt
     5941 tcggtgacgt ggctttgggc atggcgtgcg attagagccg tgcggaacga ccttggctgc
     6001 gctggcggtg taacgaattg tatcgtccgt gcacacgacg caatgtcccg ttgcaatcgc
     6061 gacgccgcgc gccgaacgcg ccgtcgacaa tgcggccgaa gctgtcgtgt ccgacgcgcc
     6121 ggaacggccg gttgcgcgga tgactcgact tcacggagaa cgggaatcat gaccaagcga
     6181 tcggcttcac ggcttgccgc cgtcgcggcg gcaacgctca tcggattgac gggcgcggcg
     6241 ttcgcgaagc cctttcatca cggccacggt gcgggtggcg acgtcgtgat gagcatcatg
     6301 cggctcaaga gccagctcaa cctcaacacg tcgcagcaga cgatgtggga caacgccgtg
     6361 gcggcaggca aggcggcccg cgtgacggcg cgcaccaaca tgcagaaggt gcacgacacg
     6421 cttgcgagcg agcttgcgaa acccgagccc gaccttgcgg cggtctcggc tgcatcggac
     6481 agtgcgcgca ctgcgaacgc ggctctgcat gcgcaggtgc gcgacgcgtg gctcaacgtc
     6541 tactcgacgt tcacgccaga gcagaagggc gtcgtgaaga gcgcgttgtc gaaccgcatg
     6601 gcgaagatgg agcagtttcg cgagaagatg ctgcagcggc actcgaacgg cggctgattc
     6661 cgagacgact tcacgagccg gcggacgccg gcctctccgg atgggcgcga gcgatcgcgc
     6721 ccttttttcg tcgatgttcc cgctgatccg taacgccgga ccatgatggg ggccggcgcg
     6781 tgcacgctag gccggcagca tagccacggg ccgctgcgcc atcagcgcga tggccgtggc
     6841 gtgcagcgtc gccgcgacga ccggtttcgg cagcagcatc aatgccgcgg cgcgcgcttc
     6901 ggtgtcggcg cccgttccgg tgtctcccga cacgatgatg gcaggaatgg cgacaccgag
     6961 ctcgtggcgc aattggcgca cggcgtcgat gcccgagcga ttgttcgcaa
//
LOCUS       DASSVX010000252         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_527896, whole genome shotgun sequence.
ACCESSION   DASSVX010000252 DASSVX010000000
VERSION     DASSVX010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527896"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFO53_10820"
     CDS             complement(<1..680)
                     /locus_tag="VFO53_10820"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8876810.1"
                     /translation="MSAPDEFTGERFLPGVVGEIAYEHCHRYAFARRFVAGRRVLDAA
                     CGEGYGAALLAPVAQSVIGVDIEPRVVADAAARYGQRGNIVFEAASVTKLPLRDASID
                     VIVSFETVEHLPAADQPQMIAEFARVLAPGGILILSSPNRPQYSDARGYRNPFHLCEL
                     DRDELAQRLGSAFAHRQWFGQRRYLGSAIWSDAIGERFEALSGSAMDVQAAAAPAPLY
                     YIVIAARSA"
     gene            complement(753..1712)
                     /locus_tag="VFO53_10825"
     CDS             complement(753..1712)
                     /locus_tag="VFO53_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876811.1"
                     /translation="MSSKTPSTGARVSTPGPRRRVALRRQLAECVLLPAIGAALPWPI
                     AWRVLRGLAARAGLFDDETMRAQRTCDAQRFVHDRPAWLQRHRLTRMVDQADAAISAT
                     RGDRWMVRHLVVEGDALPAGPCVFVGFHYGTGFWSLRHLRRLGHPVSFLSAPIDAAQW
                     RGEPLRLAFMRWRQKRVAGAGGAPIVYVGGSADRIRAALRSGTSVLALIDVPEPTTPT
                     VTVRLLEHDVQFPDGILRIGAAERVPIFGYLASLDARDGTRCLRFTRLPDDPTQALHA
                     LAAMLDAAIRRDPAAWHFWGEWPRFAKRVRSESAGESGIAGLR"
     gene            complement(1709..3382)
                     /locus_tag="VFO53_10830"
     CDS             complement(1709..3382)
                     /locus_tag="VFO53_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876812.1"
                     /translation="MASIADRQLHQSQRRGASVSTVAFALLAAASITAGVYLRATQLS
                     TQILLDDEWHAIHKLLHSDVKGIVTSFGVADYSIPLTLYYRFLALHGGLSEWQMHVPM
                     LLAGITLLVAGPWLLRRDAPPAALAAWVALLAISPLLVYLSRTARPYALTCLLAFIAV
                     VAFRNAWSGRSHAKAWGGTYAACTVLAGWLHLVTLPFTLLPFAYYAVAAFRPRRRESD
                     RNRAQALHRLVYLGAATAIPLAVVLVPPFAASAAQLALKAGAHGVTIDSMYRTTLMLF
                     GIASPGLLAACVTLGAIGAYRHARRDASFVRYIAFLCAGSAVAIAAARPQWIQHPLVY
                     ARYLLPALPFALLFVAEGAVVLVGWLRPAGLAPAALALGAVLLYEAGPIPAYLYRPNQ
                     FMGHARFQFDYDPAHNPYVQEIPRDPMPAFYRSLAQQPPASLTLIEAPWRLESNFIPF
                     PWYQQIHRQYVRIGLVTPVCGVRDFGEYPATGTGLDFRYFVHLSEILAGRTYGADYLV
                     MHLTPWKTPPDAAVEWPDVAGCLPAIAAKLGAPVYRDDSIVVFDLRASR"
BASE COUNT          650 a         1163 c         1224 g          473 t
ORIGIN      
        1 gcgctgcgcg cggcgatcac gatgtaataa agcggagccg gtgccgcggc cgcctgcaca
       61 tccatcgccg atcccgacag cgcctcgaat cgttcgccga tcgcgtcgct ccatatcgcc
      121 gagccgagat agcggcgctg cccgaaccat tgccgatgcg cgaacgcgga ccccaggcgc
      181 tgcgccagct cgtcgcggtc gagctcgcaa agatgaaacg ggttgcggta gccgcgcgcg
      241 tcgctgtatt gaggacggtt gggcgacgac aggatcagga tgccgccggg tgccagcacg
      301 cgcgcgaact cggcaatcat ctgcggctgg tcggccgccg gaagatgctc gaccgtttcg
      361 aacgagacga tgacgtcgat gctggcgtcg cgcagcggca gcttcgtgac ggacgccgcc
      421 tcgaagacga tgttgccgcg ttgtccgtat cgcgccgcgg cgtcggcaac cacgcggggc
      481 tcgatgtcga cgccaatcac gctttgcgcc accggcgcga gcagcgccgc cccgtagcct
      541 tcgccgcagg cggcatcgag cacccgccgg ccggcaacga agcggcgcgc gaacgcgtag
      601 cgatggcaat gctcgtaggc gatttccccg acaacgccgg gaaggaaccg ctcgcccgta
      661 aactcgtcgg gtgcgctcaa attttcggag gtatcgatga acggaagcgg caagacaagc
      721 gttcgcgtgc cgccgaaccg caaccttcga ttttaccgaa gcccggcgat gccgctttcg
      781 ccggccgatt cggagcgaac gcgcttcgcg aaacgcggcc attcgcccca gaagtgccag
      841 gccgcgggat cgcgccggat cgcggcgtcg agcatggcgg cgagcgcgtg cagcgcctgc
      901 gtcggatcgt ccggcaggcg cgtgaagcgc agacaccgtg tcccgtcgcg cgcgtcgagc
      961 gatgcgagat aaccgaagat cggtacgcgt tccgccgcgc cgatgcgcag gattccgtcc
     1021 gggaactgca cgtcgtgctc gagcaggcgc acggtcacgg tgggcgtcgt cggctccggc
     1081 acgtcgatca gcgcgagaac ggaggtacca ctgcgcaatg ccgcgcgaat tctgtcggcg
     1141 ctgccgccga cgtagacgat cggcgcgccg cccgcaccgg cgacgcgttt ctggcgccag
     1201 cgcatgaacg caaggcgcag cggctcgcct cgccactgcg ccgcatcgat cggcgccgag
     1261 aggaacgaca ccggatgccc gagccttcga agatgacgca gcgaccagaa gccggtgccg
     1321 taatgaaagc cgacgaagac gcacggtccc gcaggaagcg catctccctc gacgacgagg
     1381 tggcgcacca tccagcgatc gccgcgcgtc gccgatattg cggcgtcggc ctggtcgacc
     1441 atccgcgtga ggcgatggcg ctgcagccac gccgggcgat cgtgcacgaa gcgctgcgca
     1501 tcgcacgtcc gctgggcgcg catcgtctcg tcgtcgaaga ggcccgcgcg cgcggccagc
     1561 ccgcgcagca cgcgccacgc aatcggccac ggcagcgcag cgccgattgc cggcaataaa
     1621 acgcattcgg cgagctggcg gcgcaatgcc acccggcgtc ggggacccgg cgtcgacacg
     1681 cgcgcgcccg tgctcggcgt tttcgagctc atcgcgatgc ccgcaagtcg aaaacgacga
     1741 tcgaatcgtc gcgatacacc ggtgcgccga gtttcgccgc gatcgccggc aggcagccgg
     1801 ccacatcggg ccactcgacc gctgcatcgg gtggcgtctt ccacggcgtg agatgcatga
     1861 cgagatagtc ggcgccgtac gtgcgtcccg cgaggatttc ggagagatgc acgaaatagc
     1921 ggaaatcgag accggtgccc gtcgctggat actctccaaa atcgcgcacg ccgcaaaccg
     1981 gcgtcacgag cccgatcctg acgtactggc gatgtatctg ctgataccag gggaacggaa
     2041 tgaagttcga ctcgagccgc cacggcgctt cgatcagcgt cagcgatgcc ggtggctgct
     2101 gcgcgagcga gcgatagaac gcgggcatcg gatcgcgcgg aatctcctgc acgtacgggt
     2161 tgtgcgccgg atcgtagtcg aactggaatc gcgcatgccc catgaactgg ttcggccggt
     2221 acagatacgc gggaatcggt ccggcttcgt aaagcagcac ggcgccgagt gcaagcgccg
     2281 ccggcgcgag ccctgcgggc cgcaaccatc cgacaagcac gacggcaccc tcggcgacga
     2341 acagcagtgc aaagggcagc gcaggcagca gatagcgggc atagaccagc ggatgctgga
     2401 tccactgcgg gcgcgcagcg gcgatcgcga ccgcgctgcc ggcgcaaagg aacgcgatgt
     2461 aacgcacgaa cgacgcgtcg cggcgtgcgt gccgatacgc gccaatcgcg ccgagcgtaa
     2521 cgcaggcggc gagcagtccg ggcgacgcga tgccgaagag catcagcgtc gtccggtaca
     2581 tgctgtcgat cgtgacgccg tgcgcgccgg ccttcagcgc aagctgcgcg gcgctcgcgg
     2641 cgaacggggg cacgagaacg acggcaagcg ggatcgcggt cgctgcaccg aggtacacga
     2701 gccggtggag ggcttgcgcg cggttgcggt cactttcgcg ccgccgcggc cggaatgccg
     2761 caacggcgta atacgcaaac gggagaaggg tgaaaggaag cgtaacgaga tgcagccagc
     2821 cggcgagaac cgtgcaggcg gcgtacgtcc ctccccaagc cttcgcgtgc gagcggccgc
     2881 tccatgcgtt gcgaaatgcg acgacggcga tgaacgccag caggcaggtg agcgcatacg
     2941 gacgcgccgt gcggctcaga tatacgagga gcggcgaaat ggcgagcagt gccacccagg
     3001 cggcgagtgc cgcgggcggg gcgtctcgcc gcagcagcca cggaccggcc acgaggagcg
     3061 tgattcccgc aagcagcatc ggcacgtgca tctgccactc gctcaagccc ccatgcagcg
     3121 caaggaagcg gtaataaagc gtcagcggga tgctgtagtc ggcgacgccg aagctcgtga
     3181 cgatgccctt gacgtccgag tgaagcagct tgtggatcgc atgccactcg tcgtcgagca
     3241 ggatctgcgt cgacaactgc gtggcgcgca ggtacacgcc ggcggtgatc gacgcggcag
     3301 cgagcagggc gaacgcaacc gtggaaacgc tcgcgcccct gcgctggctt tggtgcagct
     3361 gccgatcagc gatcgatgcc atcgtgccag cggtgcacga ggtggacgag cccgagctcg
     3421 cgcgtgcggc cgcgcacgac gagcgggcgc tcgacgttgt cgaacacgcg cagaccctcg
     3481 gggtcggccg cgtgcgcctt gatccagtat
//
LOCUS       DASSVX010000253        26459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_144927, whole genome shotgun sequence.
ACCESSION   DASSVX010000253 DASSVX010000000
VERSION     DASSVX010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 26459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26459
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144927"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFO53_10835"
     CDS             <1..408
                     /locus_tag="VFO53_10835"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET8876813.1"
                     /translation="GEILTLDMGGPVRIVDLARDMIRLYGADPDHVPIVFTGLRPGEK
                     LYEEPLTSEEATKPTTHPKLHIAQARAANRDAVGQMVAWCERDRVADDGEVRARMRAW
                     IPEYAPVAGASLKPIPADGVESVAVPLRAPRRR"
     gene            complement(341..1687)
                     /locus_tag="VFO53_10840"
     CDS             complement(341..1687)
                     /locus_tag="VFO53_10840"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET8876814.1"
                     /translation="MADPINRSLLAFASAYLFLLPTNAATFAVSVVYGGALLCALAVY
                     VRAMRDPAARIAMAGASILVPLALWSLWSCASLAWSADPAYSRGQLAREIMDSLAVMF
                     IFYVAGREAHGLRVLIGAALASFACFALLAIGIRVATGGWDPGRFHHGVGAWATWIVM
                     VAPFLFALIVPPPAGFGRGVRPLVIGFVLLALMIATARMTDNRIVWIALAAVFATASL
                     IAAIRWPQTFIRTPLRWLAPLCVLLIVLGLAFADTVEERAQMSANGNVAVSIERDPRL
                     VLWEHVIGYLEARPLTGYGFGRLILEKRLTAETGNALLSHPHNVFASQWLQTGLVGMI
                     AFAAFVAALMLRYVRFARSRDDTLAFVGVIGIAVLAGFVTKNLTDDFLFRSNAKELWA
                     MTAFLLGYGVRRERALAESLRTLAASVLPDDGGALNAAGAPAAAPQQTPPRRPESA"
     gene            1783..2820
                     /locus_tag="VFO53_10845"
     CDS             1783..2820
                     /locus_tag="VFO53_10845"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET8876815.1"
                     /translation="MRVLIVKRDKIGDLLLTTPILAHLASALPRAEIHLLANDYNAWV
                     VAGHPALTRLWVYPRVRHEGRLRPTAAFAHLPLAWRLRRARFDWAIAMGGEESPRAIR
                     RAIATGARRVVAYAADPARYGRGLTDPLPIPSAGHEIARMTALLAPLGIAAAGRMAAP
                     VYRWPAAAEAFARRWLAAHGLARGGYIVLGIGARFACKQPTLEQIVRWSALFHARWRL
                     STVFLWTPGRDDAPLYPGDDALAARVVASGVPHFHPHRGTLAQALALIASARTTIIPD
                     SGLMHFAAASAGGVLGLFADPRAAGAAERWGPVGPRARYLEARTSVAELADDAVVAAL
                     DALIADSPVVA"
     gene            2945..4849
                     /gene="asnB"
                     /locus_tag="VFO53_10850"
     CDS             2945..4849
                     /gene="asnB"
                     /locus_tag="VFO53_10850"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET8876816.1"
                     /translation="MCGIAGFVSEAAFAADTLAAMTRCLAHRGPDAHGLFSRGPVHLG
                     HRRLSVIDIAGSAQPMSSASGDLTVVFNGEIYNFRALRAELADRGWRFESAGDTEVLL
                     AGWHAWGEHLVDRLRGMFAFALWDARDGTLFAARDHLGVKPFHYAWDGATLVFGSEIK
                     SLIAHPAVSRDVDLTALRLYLECQFIPAPHAIFAGIRKLAPGHALCLRNRTLEISRFW
                     LPDYRRKFAFDDEEATDALDRELRASVGGMLVADVPVGAFVSGGVDSGLIAAVMTDVR
                     GAPIDTFNIGFEGDVVSEHREAAAVARHIGSRHHAVMLGPDHVLDAFDHWMDIFDEPF
                     ADQAALPTMLLAGYARKHVTVVLTGEGADEVFGGYANYRKRERDERYLRWLAGPPSPL
                     PALVRLLPARLRKDRVLRALAEPLRRRYRTIPSVFDTLTQPSLLSAELLAATAASPDV
                     GDFAAQAYAECNSEQYLDRLLHIDTRLWLPDDLLTKVDRATMAHSLEARVPFLDHRFV
                     EFCARLDGRLKIRGATHKFLLKRLASRYLPPEIVSRGKQGFVMPLREWLTGRLSGQLE
                     SHLLAGGLARRGLFRAGALERLLADHRGGRREHAGRLWALLILERWFRRYAPDWRMSS
                     DPIASASGRP"
     gene            4846..5955
                     /locus_tag="VFO53_10855"
     CDS             4846..5955
                     /locus_tag="VFO53_10855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013183136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8876817.1"
                     /translation="MKLRVVHTEASTGWGGQELRILTEMEGMARRGHRVHLLAPANAQ
                     IAAAARARGLAVEALPIEWKRLPGLLALRGWLARHGGEFDVINAHSSTDAWLCALARA
                     TLPRAPRIVRTRHLSTPVNRLPTTRWLYQRATAHIVVTGKALKTALVQCNGYDPATIT
                     SVRTGIDLARFSPRDRVAMRVQCGVDARPAVGIVATLRDWKGHDYLLEAWRGLQATLP
                     GWQLLVIGDGPRRAHLAHRVRELDIGASVRFTGNQDDVPAWLACLDIAVLPSYGDEGV
                     PQSLMQAAACGLPAVSTPVGAIAEAVVHAETGLLVPPRDVPALMQALAALMRDAPMRE
                     RMGRAAHAYAQAHFGIDAMLDRMEAVFERVAAPAR"
     gene            5955..7796
                     /gene="asnB"
                     /locus_tag="VFO53_10860"
     CDS             5955..7796
                     /gene="asnB"
                     /locus_tag="VFO53_10860"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET8876818.1"
                     /translation="MCGIGGHFGRPVAAPVRARMLDALRARGPDAQHIVAFDARGRIA
                     DASLPADAALVHARLSIIDPRPVSDQPMASDDATLWLCYNGEVYGWQDEARALEARGM
                     RFRTRSDTEFILRAYEAYGIDGMLARLRGMFAFALLDLRTRRVHLVRDRLGLKPLVYA
                     HIGGTLAFGSTVRSVLPWLPDARRRLSPEAIDAYLAHRYVPAPRTIFADIARLPNAHR
                     LEYALDSGDIAQFRYWVPESAPNADCAALLDESIALRLVADRPLGLFLSGGVDSTTIA
                     SRLAATGHSGLRAFCAAFPGSSYDESPYANATADALGFPISDVAIPTTIRDDFATIVA
                     ALDEPFADPSAFPTWYLARTASTEVKVVLGGDGGDELFGGYKRVTKHLRNRWRGALRM
                     PLPTLADPKPKGWRKVACELSLDWESAYALRFSGLTPNQRRFLQPGMRHLPAHYWRKP
                     DVDATSPAERLLRWDFANFLPEYALRKSDLCTMAHGLELRAPLLDHRFVDAIVALPRE
                     LRFTRPPKRLLASLAPGLDRLGVFARKKRGFNPPLSDWLSLDLADRVASAGDSLASRT
                     GGQLDAARTSAMLAAYARTPALAEQVLSLLMLDECLAQLAQEAAHAA"
     gene            7807..8940
                     /locus_tag="VFO53_10865"
     CDS             7807..8940
                     /locus_tag="VFO53_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET8876819.1"
                     /translation="MRAVALALAFARWRVRRRKPADPRRILVLHHLLLGDTLMLTPLL
                     AKLRDRYPDARIVMTVAKPFAPLYAGRPYGVVVHPFDPADIATLWPLLRAPRFDLVIL
                     PADNRWSWLARGLGAAWIVGIADDRPAHKNWPVDEQIPYSDRPTAFGETAAALVPGAA
                     PRRYAAEDWPAPPDAGARIDGDYALLHVGASTPLKLWPPARWRALAEWLEQRGIHPVW
                     SVGPGEAALLDAIGVAQRQRRFAGTLALAELWHLIARARVLVAPDTGVAHLGRVVGVP
                     TVALFGPGSSVICGAGEFFADAPYRAVTVDPFPCRDQPIQFFRTVAWVRRCERMPGKP
                     PGGCERPLCMEAIDVAAVTSAIESLLGLPAPRGVSAGVAAGKD"
     gene            complement(8909..9517)
                     /locus_tag="VFO53_10870"
     CDS             complement(8909..9517)
                     /locus_tag="VFO53_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HET8876820.1"
                     /translation="MSAPFADVTGALLCEFHDARIGDARWAASAPLHRDGAWHWIEAN
                     HRFNTLLWREEDLARRTDVADAEIAANKRAIDRYNQQRNDAVERIDEMLLARIDDRVA
                     QPHAWHNAETAGAMIDRLSILALKTFHMAQAANRTDATDEQRSSWNERHARLTVQRRD
                     LAGCLDTLLSEAAAGRAYWRVYRQFKMYNDAALNPYLRQRPR"
     gene            complement(9514..11298)
                     /gene="msbA"
                     /locus_tag="VFO53_10875"
     CDS             complement(9514..11298)
                     /gene="msbA"
                     /locus_tag="VFO53_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HET8876821.1"
                     /translation="MKGTVLYVRLLHYVRPYRWVFGVTVLGMIAVAVGDLMLAYLVIP
                     IVRNFQNPDPLRTSWLPVTVVAVFLFRGVGSYISEYGMGWTGYRVVFDLRRDLIDKLL
                     RLPTPYYDTHAAGAVQSKIAFDAHQLASAASGTITNALRSTLTVIASFGYLLWINWKL
                     TLLTIVVIPIVGVVIRYFSRRLRRIAHDIQSRTGSLTRVLEEMIVGHRVVRIFGGEAY
                     ERDRAVAAANRLRVSMSRESAAAAASSPLTVFLAACAVGFIIWVALGQNAGGALDFPV
                     FLSYVVALLTLLDRLKGLSGINASIQRGLAAAESIFGLLDHEEESDTGTVVLDHCRGE
                     VRFDRVSLRYPGNERYSLTDVSLCVAPGETVALVGASGSGKTSLVNLIPRFYEPSAGR
                     LFIDGHDVSTLQRASLRSQIAMVSQDVLLFNDTVAANIAYGAMGGASREAIEAAASAA
                     HALDFIRLLPEGFDTQVGEHGIRLSGGQRQRVAIARALLKNAPLLILDEATSSLDSES
                     ERLVQDALEELMRGRTTIVIAHRLSTIEHADRIIVLEHGRIAETGTHRELLQRDRIYA
                     RLHRTQMVDVHGAGRVSAGDYEVLDGRR"
     gene            complement(11423..12589)
                     /locus_tag="VFO53_10880"
     CDS             complement(11423..12589)
                     /locus_tag="VFO53_10880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8876822.1"
                     /translation="MRLAIVRQRYTPFGGAERFVERALHALAQRGVELTLLTRRWPGG
                     AQRLFTPVIVNPPYVGRLWRDRGFARAVRATLAASGATLVQSHERIDCCDIYRAGDGL
                     HSVWLDERLAGAGALERIRVRASPYHRYVLDAERRLFASERLQRVICISRMVQGDIRE
                     RFGLAIERLPVIYNAIDGDEFNPRLAEGRAATRATLRIGVDRVVFLLVGSGYERKGVG
                     RALEALAGVPAPAHLVVVGHDRNPARYVALARRLGVAGRVTFAGPQADPRPYYGASDV
                     FVLPTLYDPLSNAVLEALACGLPVITSTRCGAGELVALHGAGVICGARDIAALGGAMR
                     DLCAPAVRERMAATARDAVAGLTPAAMAARLVDLYASLLDPAAGPGDAHAAALY"
     gene            complement(12591..13712)
                     /gene="rfaQ"
                     /locus_tag="VFO53_10885"
     CDS             complement(12591..13712)
                     /gene="rfaQ"
                     /locus_tag="VFO53_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HET8876823.1"
                     /translation="MSSYLPDAVPLSEIRRALVTKLRHHGDVLLASPVFTTLARAAPH
                     VEIDALVYAETVPMLANHPAIAQIHAIDRDAKRRGVLAQARAEQKLLRALRARRYDLL
                     VHLTEHPRGLVLAHLLRPRYAVTRERAGGARLWRTRFTHFYRLPRQTPRHAVEANLDA
                     LRRIGVYPAQADKKLVLVPGERAHARAEALLREHALAPRAFVHAHPGSRWFFKCWPAA
                     STAALFERIAGDGHRIAVTAAPDPRERALVDAIVAALSPAARARLIDLGGQLALPELA
                     ALTSHARAFVGVDSAPMHIAAAMQTPTLALFGPSGEFEWGPWMVEQRVVVSHAHPCRP
                     CGIDGCGGGKVSECLTALPVDHVHQQFRALVAATAARAL"
     gene            13849..14757
                     /gene="argB"
                     /locus_tag="VFO53_10890"
     CDS             13849..14757
                     /gene="argB"
                     /locus_tag="VFO53_10890"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET8876824.1"
                     /translation="MPIDAVNAAVKAQLLAEALPYIRRFHDRTIVVKYGGNAMTEAHL
                     KAGFARDVVMLKLVGMNPVIVHGGGPQIGDLLARMGIRSEFRQGMRVTDEKVMNVVEM
                     VLGELNQEIVGLINQQGGKAVGLTGQDGAFIRARKLLLRSETDPGQLIDIGLVGEIER
                     IDAELISLLDSRDFIPVIAPIGVGAEGEAYNINADLVAGKLAETLRAEKLVLMTNTVG
                     VLDRGGRLLTGLTASEIDALFADGTIHGGMLPKIGSALDAVRNGVKSSHIIDGRVDHA
                     LLLEVLTNEGVGTMIRADDAPAGGTS"
     gene            14757..15344
                     /gene="slmA"
                     /locus_tag="VFO53_10895"
     CDS             14757..15344
                     /gene="slmA"
                     /locus_tag="VFO53_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HET8876825.1"
                     /translation="MDAPRRTAPKPGERRLQILQTLATMLEAPRGEKVTTAALAARLD
                     VSEAALYRHFASKAQMFEGLIEFIESTVFSLVNKIQSESEDGAAQAEQMVVMLLRFAE
                     KNQGMTRVLIGDALVNEDERLQVRINQLTDKLEATLRQALRIAQAANGWHGDANADAA
                     VLMAYVVGRWQLFAKSGFKRSPQDGWPLQRKFLFG"
     gene            15341..16462
                     /locus_tag="VFO53_10900"
     CDS             15341..16462
                     /locus_tag="VFO53_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876826.1"
                     /translation="MNVTSHVGRALGAVRPLATAIATAIAASIALAQPLPMPPPLPAP
                     RQVTPVGPPVAQPPLPSPPPGTLGQSAQGAEARVNEPPPSRWQAALNELDADLRLRLL
                     TADMPPAAWLAGELDTTDIASQVRHYAAARTSAPQERLYQASLATACLVRVQPHLAAC
                     EAVDRLADWARRDTGNGVPAVLLADRARQRGEFDSAASYVEEAASAPRFDDYWSLGAL
                     AWWNYLRPLSLAIDPAAKAEAAANYASARDLAWAPSLHGLCIETHGRTDRMQAACAKL
                     GLAMMQRGATFALRRSGASIAEANVAEGTARAAMRSSHARIIAATARCAQAQPDFTTA
                     LESPSAATRSRGVEQFGAWAAAQARDGEVGACERLIAKR"
     gene            complement(16480..17253)
                     /gene="moeB"
                     /locus_tag="VFO53_10905"
     CDS             complement(16480..17253)
                     /gene="moeB"
                     /locus_tag="VFO53_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET8876827.1"
                     /translation="MDDQQLLRYSRHILLDELGPDAQDKFAAAHALVIGIGGLGAPVA
                     QFLAAAGVGTMTLCDADQVDLTNLQRQILYATADIGQQKVAAAASRLAAINPTVELRP
                     IAARVGGDALAALVAKADVVVDCCDNFATRHDVNRACVAARKPLVSGAAIRFDGQVAV
                     FDARDVSSPCYHCLFGEGDELEETRCATMGVFAPVVGIVGATQAAEALKLLAGIGESL
                     AGRLLLLDALTMQWRELRVPRDPGCPVCGRLTGSETIQR"
     gene            complement(17393..18811)
                     /locus_tag="VFO53_10910"
     CDS             complement(17393..18811)
                     /locus_tag="VFO53_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET8876828.1"
                     /translation="MRERMKKAGWIGMGAVLGLLLSLNLSVFAQRETRLPIPYEDLQL
                     LSAVFGKIKSDYVEPVSDDKLIKEAINGMVHGLDPHSDFLDAEAFHELQVDTQGKFGG
                     LGIEVGVEDGVIRVVSPIEDTPAFKAGIKSGDLIVKIDDTATRGMPLSKAVEKMRGAP
                     GTPVLLTVVRKDVDTPLTFTILRQEINVKSVRAKMLEPGYGFIRVRKFQEDTGSDLAK
                     AIKDLYAKGPLKGLVLDLRSDPGGLLNQAVAVSSAFLPKDALVVYTDGRTPDAHMRLT
                     ANRENYVRRGDDYLRDLPAGIKTVPMVVIVDGGTASASEIVAGALQDHKRAVVLGAQT
                     FGKGSVQTILPLGNNTGLKLTTARYYTPAGRSIQAKGIVPDFLVDDGRDSPNRIREAN
                     LEHHLDVPGDVSTTGKPGAKDAAAAIPEVKPVPATGGKIDALPPRLDFGSDDDFQLKQ
                     AVNHLKGLPVIDSVKLQSAAAK"
     gene            complement(19145..20743)
                     /gene="gpmI"
                     /locus_tag="VFO53_10915"
     CDS             complement(19145..20743)
                     /gene="gpmI"
                     /locus_tag="VFO53_10915"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET8876829.1"
                     /translation="MDVKVPPSPSAVVEPQPALRAVHRVVLLILDGFGCRADAPDNAI
                     ARADAPNWRQLLATCPHTTIDASELRVGLPEGQMGNSEVGHLNIGAGRIVYQDFTRID
                     VAIRDGQFARNPVLADAVETARAGGATLHVLGLLSPGGVHSHERQIQAMVEMAATGGA
                     PRIAVHAFLDGRDTPPRSAGASLDAMEHVCRRHPHARIASICGRYYAMDRDKRWERVA
                     PAYALVADGRAAHVAESARAALEAAYARGESDEFVQATAIGDAQGRTTRMDDGDVAVF
                     MNFRADRARELTRALTDPGFDAFDRPRVPKLSRFVCLTNYGDDFAGLPVAFAPQVIEN
                     SFGEYLARLHLAQLRIAETEKYAHVTYFFNGGSEAVYPGEDRVLVASPRVATYDQKPE
                     MSAPEVTDRLVEAIEGERYDAIICNYANADMVGHTGNFEAAKRAIETLDACIGRVVDA
                     ARAHGTDVIITSDHGNAERMHDETTGQAHTAHTRNLVPFVYVGRPASMMSDGGALQDV
                     APTLLAVMGLPKPPQMTGHSLLRF"
     gene            20829..21251
                     /locus_tag="VFO53_10920"
     CDS             20829..21251
                     /locus_tag="VFO53_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET8876830.1"
                     /translation="MPIIEGFVIRNWPLVLVFVISGALLLWPLVQRRLAPGREISTLD
                     ATRLLNKEQPLVLDVREANELAGGKLPNATHIPLSQLKDRGGEIAKHAARPVIVYCDR
                     GARAGAAVAALTRLGFTHVQSLRGGFRAWKDAGLPLSK"
     gene            21268..21537
                     /gene="grxC"
                     /locus_tag="VFO53_10925"
     CDS             21268..21537
                     /gene="grxC"
                     /locus_tag="VFO53_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HET8876831.1"
                     /translation="MRPQVTMYTSAYCGYCRRAERLLETKGVTGIAMIRVDEQPEARM
                     AMIERTGRRTVPQIYIGDHHVGGYDELAALDRAGRLNGLLGGEAA"
     gene            21593..22093
                     /gene="secB"
                     /locus_tag="VFO53_10930"
     CDS             21593..22093
                     /gene="secB"
                     /locus_tag="VFO53_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET8876832.1"
                     /translation="MADSPATNADSQPAAAPAGVSAQFSIEKIYVKDLSLENPGAPQS
                     FQMTEAPQIEIGLRTRAEQVAQDTYECVLTITVTARSGDKTVFLVEAAQAGLFAIRGV
                     PANQLQPVLAIHCPAVLFPYARETVASATIHAGFPPVHLAPINFDALYQQQLAQAAAG
                     APATAN"
     gene            22090..22572
                     /locus_tag="VFO53_10935"
     CDS             22090..22572
                     /locus_tag="VFO53_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET8876833.1"
                     /translation="MIRTGVPYIAARALAIALPLLAGVASAADFRSTRDASTVLYDAP
                     SARAKPLFVYGRDVPVESLVSVEGWTKVRDASGTIGWMPSKSLADKRMLIVRAPGADV
                     RAAPDDKAAVVFRAEPDVLLELAEPAASPAATATPGWVKVRHRDGQTGYARLAQVFGL
                     "
     gene            22589..23590
                     /locus_tag="VFO53_10940"
     CDS             22589..23590
                     /locus_tag="VFO53_10940"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET8876834.1"
                     /translation="MSANVAVLGGGAWGTAFAVHLAARTHAPSRVTLYMRDPAQALAV
                     HAARENRRYLRGVRLPDALAVVSDLAGIRADVLFAATPVAALAPLAGALADAGVHAPL
                     VSLAKGFVAAAEPGGWTLPHRMLASRWPHPVGVVSGPTFATEVAQGLPSAVVVATNTA
                     RLTDDVAQLLRGETMRAYPSDDVTGVEVGGAVKNVLAIAAGASDGLGFGDNARAALIT
                     RGLAEAGRLSAALGGRPQTLTGLAGLGDLVLTCTGNLSRNRRVGLALSRGQPLAEILA
                     ELHHVAEGVAATRAARELALHHGVDMPIVDAVHRVLYGHVAPRDAVTALLAREPRGE"
     gene            23619..25715
                     /locus_tag="VFO53_10945"
     CDS             23619..25715
                     /locus_tag="VFO53_10945"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET8876835.1"
                     /translation="MRVALGFAMTLAISAGAAGAASPAFFAPETPARPMVDVLHGTTL
                     TDRYRWLENGKDPEVAAWTRAQHAASVQYLDRNAPPVPGMEDELAKYFDRDRTDPPLV
                     KHGRQFFQRTRRGEPQAKLYTRIDGRDVLLFDPVALDPSGRTKIGDVVPNRDATKLAV
                     GVYAQGTERKDYRIIDSTTGAQTGPAISGLDSFGWARDERFAFITPRTAESDAKQEPM
                     RCLRHRLGGDRRDDVLLMKVDDAKNYCSVYEPEDADVTVFENGDFWSNTLRIRPIGSD
                     AEPRTIYSSRQFRAEAIFRRDRMYFRTNDHAPNWKLMAASYAKPQSGDWSTLIAEQAN
                     VLADATVTSDVIVATLREDVLSRLDVYDHAGKRLRELPLPEFGNVSGIAYDLDADRAY
                     ATLASYTAPSTLYGLDGKTLAWALVWQDDPPLDLSQVAAERVYVSARDGARIPVFVVH
                     RKDMAKNGVNPTLLHVYGGFDNAVEPFYLGSYASFVNRGGIFVDAGVRGGSEYGEKWH
                     EQAMLANKQNTFDDTIAVAEWLVHEGYTKPAKLALEGGSNGGLTVGAVITQRPDLFAA
                     AICQVPLLDMVRYHRFLIARYWIPEYGDPDKDADFRWILRYSPYQNVRAGVNLPQTLV
                     VAGEYDSRVDPLHAKKFVAEVQNHPGQVSPFLLYMDFDSGHGTGKTQQQRVIDRDYEL
                     RFLMSALSMKQDDTPK"
     gene            complement(25783..26247)
                     /locus_tag="VFO53_10950"
     CDS             complement(25783..26247)
                     /locus_tag="VFO53_10950"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003220777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET8876836.1"
                     /translation="MFAVVLVHPEIPPNTGNVIRLTANAGVGLHLVEPLGFRMDDREL
                     RRAGLDYREYASVRVHRDFAACRAALDADGPRRWLAFTTQARRSSYDVAYAPGDVLTF
                     GCESQGLPAGIVAQFAADARVRIPMRPRVRSLNLSNAVAVAVYEAWRQNGFK"
     gene            complement(26257..>26459)
                     /locus_tag="VFO53_10955"
     CDS             complement(26257..>26459)
                     /locus_tag="VFO53_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET8876837.1"
                     /translation="AGLTLRERTRNVRNAFEANERVAGRAVAIVDDVMTTGATLAAAA
                     IAVRKAGAIRVEAWVVARTPPP"
BASE COUNT         4024 a         9152 c         9199 g         4084 t
ORIGIN      
        1 ggcgagatcc tgacgctcga catggggggt cccgtgcgca tcgtcgacct cgcgcgcgac
       61 atgatccgcc tctacggcgc cgacccggac cacgtcccga tcgtgttcac cggcctgcgc
      121 ccgggtgaga agctctacga ggagccgctc acctcggaag aggcgacgaa gccgacgacc
      181 catccgaagc tgcacatcgc gcaggcccgt gcggcgaacc gcgatgccgt cggccagatg
      241 gtcgcgtggt gcgagcgcga ccgtgttgcc gacgacggcg aagtgcgggc gcggatgcgg
      301 gcgtggatcc ccgagtacgc accggtcgcc ggcgcgtcgc tcaagccgat tccggccgac
      361 ggggtggagt ctgttgcggt gccgctgcgg gcgccccggc ggcgttgagc gcgccgccgt
      421 cgtccggcag caccgatgcg gcgagcgtgc gcagcgattc ggcgagcgcg cgctcgcggc
      481 gcacgccgta gcccaacagg aacgcggtca tcgcccacag ctccttggcg ttgctgcgga
      541 acaggaagtc gtcggtcaga ttcttggtga cgaatccggc gagaaccgca atcccgatga
      601 cgccgacgaa ggcgagcgtg tcgtcgcgcg agcgcgcgaa gcggacgtag cgaagcatca
      661 gcgccgcgac gaacgcggcg aatgcgatca tcccgacgag acccgtctgc agccactggc
      721 tggcgaagac gttgtgggga tgggagagga gcgcgttgcc cgtttcggcc gtcaggcgct
      781 tctcgaggat caggcggccg aagccatagc ccgtcagcgg gcgcgcctcg aggtagccga
      841 tcacatgctc ccacagcacc agccgcgggt cgcgctcgat ggaaacggcg acgttgccgt
      901 tcgccgacat ctgggcgcgc tcctcgaccg tgtccgcgaa cgcgaggccc agcacgatca
      961 gcagcacgca cagcggcgcg agccagcgca acggcgtgcg gatgaacgtc tgcggccagc
     1021 ggatcgcggc gatcagcgat gcggtcgcga acaccgccgc aagcgcaatc cagacgatcc
     1081 ggttgtcggt catgcgcgcc gtcgcgatca tcagtgcgag cagcacgaag ccgatcacca
     1141 gcgggcgcac gcctcggcca aaacccgcag gcggcggcac gatcagcgcg aagaggaacg
     1201 gcgcgaccat gacgatccac gtcgcccacg cgccgacgcc gtggtgaaag cggccgggat
     1261 cccagccgcc ggtcgcgacg cgtatcccga tcgcgagcag cgcgaagcat gcgaagctcg
     1321 cgagcgcggc gccgatcagc acgcgcaggc cgtgcgcttc gcgtccggcg acatagaaga
     1381 tgaacatgac cgcgaggctg tccatgatct cgcgcgcgag ctggccacgc gaatacgcgg
     1441 gatccgccga ccacgcaagc gacgcgcacg accacagcga ccacagcgcg agcggcacga
     1501 ggatcgacgc cccggccatc gcgatgcgcg ccgccggatc gcgcatcgcg cggacgtaaa
     1561 cggcgagcgc gcacagcaac gcgcccccgt acacgaccga gacggcgaac gtcgccgcgt
     1621 tggtgggcag caggaagagg tacgccgacg cgaaggccag cagcgagcgg ttgatggggt
     1681 ctgccacgcg cggggtttct cgagggttgc cggccgggag cgcgcgaacg cgaggcgcga
     1741 ttctaacctc cccgattccg gccctcaccg ggtcgcgacc acatgcgcgt gctgatcgtc
     1801 aagcgcgaca agatcggcga ccttttgctg acgacgccga tcctcgcgca tcttgcgtcg
     1861 gcgttgccgc gcgccgagat ccacttgctg gccaacgatt acaacgcatg ggtcgtcgcc
     1921 gggcaccccg cgctcacgcg gctatgggtc tatccgcgcg ttcggcatga gggccgtctg
     1981 cgaccgacgg ccgcgttcgc gcatctgccg ctggcgtgga ggctgcgccg tgcgcgcttc
     2041 gactgggcga tcgcgatggg cggcgaggag tcgccgcgcg cgatccgccg cgcaatcgcc
     2101 accggcgcgc gccgggtcgt tgcctacgct gccgatcccg cgcgttacgg gcgtggcctc
     2161 accgatccgc taccgatccc gtcggccgga cacgagatcg cgcggatgac ggcgttgctc
     2221 gcaccgctcg gcatcgccgc ggcgggccga atggctgcgc cggtctatcg ctggcctgcg
     2281 gcagcggagg cattcgcgcg acgctggctc gccgcgcacg gcctcgcgcg cggcggctac
     2341 atcgtgctcg gcatcggcgc acgtttcgcc tgcaagcagc cgacgctcga gcagatcgtg
     2401 cgctggagcg cgctatttca tgcacggtgg cggctttcca cggtgttcct gtggacgccg
     2461 ggccgggacg acgcgccgct gtatccgggc gatgacgcgc tcgcggcacg cgtcgtggcg
     2521 agcggagtgc cgcattttca tccgcaccgg ggaacgctgg cgcaggcgct ggcgctgatc
     2581 gccagcgcgc gcacgacgat catccccgac tccgggctca tgcatttcgc cgcggcgagc
     2641 gcgggcggcg tgctcgggct cttcgccgac ccgcgcgcgg cgggcgcagc cgagcggtgg
     2701 ggtccggtcg gtccgcgcgc gcgttatctc gaggcgcgca ccagcgtcgc cgagctcgcc
     2761 gacgatgcgg tcgtcgcagc gctcgacgcg ctgattgccg attcgccggt ggtcgcgtga
     2821 tcgcgatcgc gtcgtgcgct ttgcgccggg tccatgcggt agactccgcg ctccgcgcgg
     2881 cggtcgcgcg cgatcgcgcg cgacgctctt cgcgctccca ccgcgtcctt atccgttcat
     2941 ccgcatgtgc gggatcgcag gctttgtaag cgaagcggcg ttcgccgccg acacgctcgc
     3001 cgccatgacc cgatgtctcg cgcatcgcgg ccccgatgcg cacgggctgt tctcgcgcgg
     3061 accggttcac ctcggccatc ggcgtctttc cgtgatcgac atcgccggaa gcgcgcagcc
     3121 gatgtccagc gccagcggcg acctcacggt cgtgttcaac ggcgagatct acaatttccg
     3181 ggcgcttcgc gccgagctcg cggaccgcgg ttggcgcttc gagtcggcag gcgacacgga
     3241 agtgctgctc gccggctggc acgcgtgggg cgagcacctc gtcgatcggc tgcgcggaat
     3301 gttcgcgttc gctttgtggg atgcgcgtga cggaacgctc ttcgcggcac gcgatcatct
     3361 cggcgtcaag ccatttcact acgcgtggga tggggcgacg ctcgtgttcg gctcggagat
     3421 caagtcgctg atcgcgcatc cggccgtgtc gcgcgacgtg gatctgacgg cgctgcgtct
     3481 gtatctcgag tgccagttca ttcccgcgcc gcatgcgatt ttcgccggca tccgcaagct
     3541 cgcgccgggc cacgcgctgt gcctgcgcaa ccggacgctg gaaatcagcc gcttctggct
     3601 gcccgattac cgtcgcaagt tcgcgttcga cgacgaggag gccaccgatg cgctcgaccg
     3661 cgaattgcgg gcatcggtcg gcggcatgct ggtcgccgac gtcccggtcg gggcattcgt
     3721 cagcggcggc gtcgactcgg ggctgatcgc cgccgtgatg accgacgttc gcggcgcgcc
     3781 gatcgacacg ttcaacatcg gattcgaagg cgacgtcgtc agcgagcatc gggaagccgc
     3841 ggcggtcgcg cggcacatcg gcagccgtca ccacgccgtg atgctcggac ccgaccacgt
     3901 gctcgatgcc ttcgatcact ggatggacat cttcgacgag ccgttcgccg accaggcggc
     3961 gttgccgacg atgctgcttg ccgggtacgc ccgcaagcac gttacggtcg tgctgaccgg
     4021 cgaaggcgcc gacgaggtgt tcggcgggta tgcgaactat cgcaagcgcg agcgcgacga
     4081 acgctatctg cgctggctcg cgggcccgcc gtcgccgttg cccgcgctcg tgcggctgct
     4141 cccggcgcgc ctgcgcaagg accgcgtgct gcgcgcgctc gccgagccgc ttcggcgccg
     4201 ctatcgcacg attcccagcg tgttcgacac gctgacgcag ccgtcgctgc tgtcggccga
     4261 gctgctggcg gcgaccgccg cgtcgccgga cgtcggagat ttcgccgcgc aagcctacgc
     4321 cgaatgcaac tcggagcaat acctcgaccg gctgctgcac atcgacacgc gcctgtggct
     4381 gcccgacgat ctgctgacca aggtcgaccg cgcgacgatg gcgcattcgc tcgaggcgcg
     4441 cgtgccgttt ctcgaccacc gcttcgtcga attctgtgcg cggctcgacg gcaggctcaa
     4501 gattcgcggc gcgacgcaca agttcctgct gaagcgcctt gcgtcgcgtt acctgccgcc
     4561 ggaaatcgtg tcgcgcggca agcagggttt cgtcatgccg ctgcgcgaat ggttgaccgg
     4621 caggctgtcg ggacaactgg agtcgcatct tctggccgga ggactcgcgc gccgcggcct
     4681 gttccgcgcc ggcgcgctcg agcgtctgct cgccgatcat cgcggcggac ggcgcgagca
     4741 cgccgggcga ctttgggcgc tgctgatcct cgagcgctgg ttccgccgct acgcgccgga
     4801 ctggcggatg tcctcggacc cgatcgcatc cgcgagcggt cgaccatgaa gcttcgcgtc
     4861 gtgcataccg aggcgtcgac cggctggggc gggcaggaac tgcgcatcct gacggagatg
     4921 gaaggcatgg cgcggcgcgg gcaccgggtg cacctgctcg caccggcgaa tgcgcagatc
     4981 gcggccgccg cacgcgcacg cggcctcgct gtcgaagcgt tgccgatcga gtggaagcgc
     5041 ttgccgggcc ttctcgcgct gcgcggctgg ctcgctcgac acggtggcga gttcgacgtg
     5101 atcaacgcgc acagctcgac cgatgcgtgg ctttgtgcgc tggcccgcgc gacgctgccg
     5161 cgcgcgccgc gcatcgtgcg cacgcgtcac ctgtcgacac cggtcaaccg cttgccgacg
     5221 acgcgctggc tgtatcagcg cgcgacggcg cacatcgtcg tgaccggcaa ggcgctcaag
     5281 acggcgctcg tgcagtgcaa cggctacgat ccggcgacga tcacgtcggt gcgcaccggc
     5341 atcgatctcg cgcgcttttc tccgcgcgac cgcgtcgcga tgcgcgtgca atgtggcgtc
     5401 gacgcgcgtc ccgcggtcgg catcgtggcg acgttgcgcg actggaaggg tcatgactac
     5461 ctgctggaag cgtggcgcgg gttgcaggcg accctgcccg ggtggcagct gctggtgatc
     5521 ggcgacggcc cgcgccgcgc gcatctcgcg catcgcgtgc gcgaactcga catcggggcg
     5581 agcgtccgct tcaccggcaa ccaggacgac gtgcccgcgt ggctcgcgtg tctcgacatc
     5641 gccgtgttgc cgtcgtatgg cgacgaaggc gtgccgcaga gcctgatgca ggcggctgcg
     5701 tgcggcctgc ccgccgtttc gacgcccgtc ggcgccatcg ccgaagccgt cgttcacgcc
     5761 gagacggggc tgctcgttcc gccgcgcgat gtgccggcgc tcatgcaggc gcttgccgca
     5821 ttgatgcgcg atgcgccgat gcgggagcgg atgggccgcg cggcgcatgc gtacgcgcag
     5881 gcgcatttcg gcatcgacgc gatgctcgac cggatggaag cggttttcga gcgcgttgcc
     5941 gcgcccgcac gctgatgtgc ggcatcggcg gacatttcgg aaggccggtc gccgcacctg
     6001 tccgcgcgcg catgctcgac gcattgcgtg cgcgaggccc ggatgcacag cacatcgtcg
     6061 ccttcgatgc gcgaggtcgc attgcggacg cttcgttgcc ggcggatgcc gccctcgtgc
     6121 atgcgcggct gtcgatcatc gatccgcgcc cggtgtccga ccagccgatg gcgagcgacg
     6181 acgcgacgct gtggctttgc tacaacggcg aggtgtatgg ctggcaggac gaggcgcgcg
     6241 cgctcgaagc gcgcgggatg cggttccgca cgcgctccga caccgagttc atcctgcgcg
     6301 cctacgaggc gtacggcatc gacggcatgc ttgcgcgcct gcgcggaatg ttcgcgttcg
     6361 cgctgctcga cttgcgcacc cgacgcgtgc atctcgtgcg cgaccgcctc gggctgaagc
     6421 cgctcgtgta cgcgcacatc ggcggtacgc tcgccttcgg ctcgacggtg cgaagcgtgc
     6481 tgccgtggct gcccgacgcg cgccggcgcc tttcgcccga agcgatcgac gcctacctcg
     6541 cgcatcgcta cgttccggcg ccgcgcacca ttttcgccga catcgcgcgg ctccctaacg
     6601 cgcatcggct cgagtacgcg ctcgacagcg gcgacatcgc gcaattccgc tactgggtgc
     6661 ccgaaagcgc gccgaacgcc gattgcgccg cgctgctcga cgaatcgatc gcgctgcggc
     6721 tcgttgccga ccgcccgctc gggctgttcc tctccggcgg cgtcgattcg acgacgatcg
     6781 caagccggct cgcggcgacc ggccattccg gtctgcgcgc attctgtgcg gcgtttcccg
     6841 gctcgtcgta cgacgaaagc ccttacgcga atgccacggc ggacgcgctc ggattcccca
     6901 tctccgatgt cgcgatcccg acgacgatcc gcgacgattt tgcgacgatc gtcgccgcac
     6961 tcgacgagcc gtttgccgat ccttcggcgt tccccacctg gtatctcgcg cgcacggcgt
     7021 cgaccgaggt gaaggtggtg ctcggcggcg acggcggcga cgagctcttc ggcggctaca
     7081 agcgcgtgac gaagcatctg cgcaaccgct ggcgtggcgc tttgcgcatg ccgcttccca
     7141 cgctggccga tccgaagccg aagggctggc gcaaggtcgc ttgcgagctg tcgctcgact
     7201 gggaatcggc gtacgcgctg cgcttctcgg gcctgacgcc gaaccagcgc cgcttcctgc
     7261 agcccggcat gcggcacctg cccgcgcact actggcgcaa gcccgatgtc gacgccacgt
     7321 cccccgccga gcgcctgctg cgctgggact tcgcgaactt cctgcccgag tatgcgttgc
     7381 gtaaatccga tctgtgcacg atggcgcacg gtctcgagct gcgcgcgccg ctgctcgacc
     7441 accggttcgt cgatgcgatc gtggcgctcc cgcgtgaact gcggttcacg cggccgccga
     7501 agcggctgct ggcgtcgctc gcccccggac tcgatcggct cggcgttttc gcgcgcaaga
     7561 agcgcgggtt caatccgccg ctttccgact ggctgtcgct cgatctcgcc gaccgcgtgg
     7621 cgtcggcggg cgactcgctc gcgtcgcgca ccggcggaca gctcgacgct gcgcgcacgt
     7681 cggcgatgct tgcggcgtat gcccgcacgc cggcgctcgc cgagcaggtg ctgtcgctgc
     7741 tgatgctcga cgagtgcctg gcccagctcg cgcaggaggc cgcgcatgcg gcgtagccgg
     7801 gggccgatgc gcgcggtggc gctggcgctg gcgtttgcac gctggcgcgt gcgccggcgc
     7861 aagccggccg atccgcgccg cattctcgtt ctgcatcatc tgctgctcgg cgatacgctg
     7921 atgctgacgc cgctgctcgc gaagctgcgc gaccggtatc ccgacgcgcg catcgtgatg
     7981 acggtggcga agcccttcgc accgctgtac gccggacgcc cgtacggcgt agtcgtgcat
     8041 ccgttcgatc cggcagacat cgccacgctg tggccgctcc tgcgcgcgcc gcgcttcgac
     8101 ctcgtgatcc tgcccgcgga caaccgctgg tcgtggctcg cgcgcggact cggcgcggca
     8161 tggatcgtcg gcatcgccga cgaccgcccg gcgcacaaga actggcccgt cgacgagcag
     8221 attccatact ccgatcggcc gaccgcgttc ggcgagacgg cagcagcgct cgtgcccggt
     8281 gccgcgccgc gccgttacgc cgcggaggac tggccggcgc cgcccgatgc cggcgcgcgc
     8341 atcgacggcg actatgcgct gctgcacgtc ggcgcaagca cgccgctcaa gctgtggccg
     8401 cccgcgcgct ggcgtgcgct cgccgagtgg ctcgagcagc gaggcattca tccggtgtgg
     8461 agcgtcggtc ccggagaggc agctctcctc gacgccatcg gcgttgcgca gcgccagcgg
     8521 cgattcgccg gcacgctggc gctggcggag ctatggcatc tgatcgcgcg ggcacgcgtg
     8581 ctcgtcgcgc ccgacaccgg cgtcgctcac ctcggacgcg tggtcggggt gccgacggtc
     8641 gcgttgttcg ggccgggttc gagcgtgatc tgcggcgcag gcgagttctt tgcggacgcg
     8701 ccgtaccgcg cggtgaccgt cgatcccttc ccttgccgcg atcagccgat ccagttcttc
     8761 cgtacggtcg cgtgggtgcg gcgctgcgag cggatgccgg gcaagccgcc gggcggttgc
     8821 gagcggccgc tttgcatgga ggcgatcgac gtcgcggccg tcacgtcggc gatcgagtcg
     8881 ctgctcgggc tgccggcgcc gcgcggcgtc agcgcgggcg ttgccgcagg taaggattga
     8941 gtgccgcgtc gttgtacatc ttgaactgac gatagacccg ccagtacgcg cgccccgccg
     9001 cggcttccga cagcagcgtg tcgaggcagc ctgcaagatc gcgacgctgc acggtcaatc
     9061 gcgcgtggcg ctcgttccag gagcttcgtt gttcgtcggt cgcatcggtc cgatttgccg
     9121 cctgcgccat gtgaaacgtc ttcagcgcaa ggatcgacag gcggtcgatc atcgcgccag
     9181 cggtttcggc gttgtgccat gcgtgtggct gcgccacccg gtcgtcgatg cgcgccagca
     9241 gcatctcgtc gatccgctcg acggcgtcgt tgcgttgctg gttgtagcgg tcgatcgcgc
     9301 gcttgttggc ggcgatctcg gcatccgcga cgtccgtccg ccgtgcgaga tcctcctcgc
     9361 gccagagcag cgtgttgaac cggtgattcg cctcgatcca gtgccacgca ccatcgcgat
     9421 gcagcggcgc cgatgccgcc catcgcgcgt cgccgatccg cgcgtcgtgg aactcgcaca
     9481 acagcgcgcc ggtgacgtcg gcgaacggcg cgctcatcgg cgaccgtcga ggacttcgta
     9541 atcgccggcg ctcacgcggc cggcgccgtg cacatcgacc atctgcgtgc ggtgcaggcg
     9601 cgcgtagatc cggtcgcgct gcagcagctc gcgatgcgtg cccgtttcgg cgatgcgtcc
     9661 gtgctcgagc acgatgatgc ggtccgcgtg ctcgatcgtc gacagccgat gcgcgatgac
     9721 gatcgtcgtg cgcccgcgca tcaattcctc gagcgcgtcc tggaccagac gctccgattc
     9781 cgaatcgagc gacgacgtcg cttcgtcgag gatcagaagc ggcgcgttct tgagcaacgc
     9841 gcgtgcgatg gcgacgcgct ggcgctgccc gcccgacagc cggatgccgt gctcgccgac
     9901 ctgcgtgtcg aacccttccg gcagaagacg gatgaaatcg agcgcgtgcg ccgccgatgc
     9961 cgcggcctcg atcgcctcgc ggctcgcgcc acccatcgcg ccgtacgcga tgttcgcggc
    10021 gaccgtgtcg ttgaacagca gcacatcctg cgacaccatc gcgatctgcg agcgcaggct
    10081 cgcacgctgg agcgtcgaca cgtcgtgtcc gtcgatgaac aggcgccccg cactcggctc
    10141 gtagaagcgc gggatcaggt tgacgagcga cgtcttgccg ctgcccgacg cgccgaccag
    10201 cgcgaccgtt tcgccgggcg cgacgcaaag cgatacgtcg gtcagcgagt agcgctcgtt
    10261 gcccggatag cgcagcgaca cgcgatcgaa acggacctcg ccgcggcaat gatccagcac
    10321 gacggtgccg gtatcggatt cctcttcgtg atcgaggaga ccgaagatgc tttccgccgc
    10381 cgccagtccg cgctggatcg acgcgttgat gccggagagc cccttcagcc gatcgagcag
    10441 cgtcaggagc gcgacgacat acgacaggaa aacggggaaa tcgagcgctc ccccggcgtt
    10501 ctggccgagc gcgacccaga tgatgaagcc gacggcgcac gcggcgagga acaccgtcag
    10561 cggcgagctc gctgcggccg ccgcggattc gcgcgacatc gacacgcgaa gccgattcgc
    10621 ggccgcgacg gcgcgatcgc gctcgtaggc ctcgccgccg aatatgcgca cgacgcgatg
    10681 gccgacgatc atctcttcga gcacgcgggt cagcgagccg gtgcgcgact ggatgtcgtg
    10741 cgcgatgcgg cgcaggcgcc ggctgaagta gcggatcacc acgccgacga tcgggatcac
    10801 gacgatcgtg agcagcgtca gcttccagtt gatccacagc aggtagccga agctcgcgat
    10861 gacggtgagc gtcgagcgca gcgcgttcgt gatcgttccc gacgccgccg aggctagctg
    10921 gtgcgcatcg aacgcgatct tcgactgcac ggcgccggcg gcgtgcgtgt cgtaatacgg
    10981 cgtcggcagg cgcagcagct tgtcgatgag atcgcggcga aggtcgaaca cgacgcgata
    11041 ccccgtccag cccatcccgt actcggagat gtaggagccc acgccgcgaa agaggaagac
    11101 ggcgacgacg gtgacgggaa gccacgatgt ccgcagcgga tccgggttct ggaaattgcg
    11161 gacgatcggg atgaccaggt acgcgagcat caggtcgccc accgccacgg cgatcatgcc
    11221 gaggacggtg accccgaaga cccagcggta cggccggacg taatgcagca ggcggacgta
    11281 gaggaccgta cccttcatcg aacgaggatc cggtgtgcgg gcgccacgct acgaggcgcc
    11341 ggtggcggca ggaacgtgca ggcgatgcga aggctgcaag agaaggcgaa ggaccggcgg
    11401 gtcgggtaat ccgcagtcgg gttcagtata gcgcagcggc gtgggcgtcc cccgggccgg
    11461 cagccggatc gagcagcgat gcgtagagat cgacgagacg agcggccatc gcggcgggcg
    11521 tgaggcccgc aacggcgtcg cgcgcagtgg ccgccatgcg ctcgcgcacg gcgggcgcgc
    11581 agaggtcgcg catcgcgccg ccaagcgccg cgatgtcgcg cgccccgcag attacgccgg
    11641 cgccgtggag ggcgacgagt tcccctgccc cgcagcgcgt gctcgtgatc accggcaatc
    11701 cgcaggcgag cgcctcgagc accgcgttcg acagcggatc gtagagcgtc ggcagcacga
    11761 acacgtccga cgcgccgtag tacggcctgg ggtccgcctg cggtcccgcg aaggtcacgc
    11821 gtccggcgac gccaagccgg cgcgcgagcg cgacatagcg cgccgggttg cggtcgtgac
    11881 cgacgacgac gaggtgcgcc ggtgcgggca cgccggcaag cgcttcgagc gctcgtccca
    11941 cgcccttgcg ttcgtatccg gaaccgacga gcaggaagac cacccggtcg acgccgatgc
    12001 gcaacgtcgc ccgcgtcgcg gcgcgccctt ccgcgagccg cgggttgaac tcgtcgccgt
    12061 cgatcgcgtt gtagatgacc ggcaggcgct cgatcgcgag tccgaagcgc tcgcggatgt
    12121 cgccctggac catccgggaa atgcagatca cgcgctgcag ccgctcgctt gcaaacagac
    12181 gccgctcggc gtcgagcacg tagcgatggt aagggctcgc gcgcacccga attcgttcca
    12241 gcgcgcccgc gccggccagg cgctcgtcga gccacaccga atgcagcccg tcgcccgcgc
    12301 ggtagatgtc gcagcagtcg atgcgctcgt gcgactgcac gagcgtcgca cccgaggcgg
    12361 ccagcgtggc gcgaacggcg cgggcgaaac cgcgatcgcg ccacaggcgg ccgacgtaag
    12421 gcggattgac gatgaccggc gtgaacaggc gttgcgcgcc gccgggccat cgccgcgtca
    12481 gcaacgtgag ctcgacgccg cgctgcgcga gggcgtgcag cgcgcgctcg acgaagcgct
    12541 cggccccgcc gaacggcgtg tagcgctggc ggacgatcgc gagccgcact tcagagagcc
    12601 cgcgcagccg tggcggccac gagcgcgcgg aactgctggt gcacgtgatc gaccggcagc
    12661 gccgtcaggc actcggacac cttgccgcca ccgcagccgt cgatgccgca ggggcggcac
    12721 ggatgcgcgt gcgacacgac cacgcgctgc tcgaccatcc acggccccca ctcgaattcg
    12781 ccggaagggc cgaacagcgc gagcgtgggc gtctgcatcg ctgcggcaat gtgcatcggc
    12841 gccgaatcga cgccgacgaa cgcgcgtgcg tgcgacgtga gcgcggcgag ctcgggcagc
    12901 gcaagctgtc cgccgaggtc gatgagccgc gcccgcgccg cgggggagag tgccgcaacg
    12961 atcgcatcca ccagcgcgcg ctcgcgcgga tcgggcgcgg cggtcaccgc gatccggtgc
    13021 ccgtcgccgg cgatgcgctc gaagagcgcc gccgtgcttg cggcgggcca gcacttgaag
    13081 aaccagcgcg atccgggatg cgcatgcacg aacgcacgcg gcgcgagcgc atgctcgcgc
    13141 agcagcgcct cggcgcgcgc gtgggcacgt tcgccgggca cgaggacgag cttcttgtcc
    13201 gcctgcgcag gatagacgcc gatccggcgc agcgcgtcca gattcgcctc caccgcgtgg
    13261 cgcggcgtct gccgcggcag ccggtagaaa tgcgtgaagc gcgtgcgcca caggcgcgcg
    13321 ccgccggcgc gctcgcgcgt caccgcgtag cgagggcgca gaagatgcgc gagcacgagg
    13381 ccgcgcggat gctcggtcag gtggacgagc aggtcgtagc gccgggcgcg aagcgcacgc
    13441 agcagcttct gctcggcgcg cgcctgcgcc agcacaccgc ggcgctttgc gtcacggtcg
    13501 atcgcgtgga tttgcgcgat cgccgggtga ttcgcgagca tcggcaccgt ctcggcgtag
    13561 acgagcgcgt cgatctcgac atgcggcgcc gcacgcgcga gcgtggtgaa caccggggag
    13621 gcaagcagca cgtcgccgtg gtggcgcagc ttggtgacga gcgcgcggcg gatctcggac
    13681 agcgggacgg cgtccggcag gtagctcgac atgggcggca acaatagcaa atcgccgcca
    13741 acgcggcgcg ggacttgtca gcgctcactc gtgccacacc gcgcgctgcg ggccgatctc
    13801 gggcattcgg gttacaatcc cccgcacttc gccgccgccg atcctccgat gcccatcgat
    13861 gccgtcaacg ccgccgtcaa ggcgcagctc ctggccgagg cgttgccgta catccggcgc
    13921 tttcacgacc gcacgatcgt cgtcaagtac ggcggcaacg cgatgaccga ggcgcatctc
    13981 aaggcgggct tcgcgcgcga tgtcgtgatg ctgaagctcg tcgggatgaa tccggtgatc
    14041 gtccacggcg gcggcccgca gatcggcgat ctcctggctc gcatgggaat ccgcagcgaa
    14101 ttccgccagg gcatgcgcgt gaccgacgag aaggtgatga acgtcgtcga gatggtgctg
    14161 ggcgagctca atcaggaaat cgtcgggctc atcaaccagc agggtggcaa ggccgtgggt
    14221 ctcaccgggc aggacggcgc gttcatccgc gcgcgcaagc tgctgctgag aagcgaaacc
    14281 gaccccgggc agctcatcga catcggcctc gtcggggaaa tcgagcgcat cgacgccgag
    14341 ctcatcagcc tgctcgattc acgcgacttc attccggtga tcgcgccgat cggcgtcggg
    14401 gccgagggcg aggcgtacaa catcaacgcc gatctcgtcg ccggcaagct cgccgaaacg
    14461 ctgcgggcgg aaaaactcgt gctcatgacg aacacggtcg gcgtgctcga ccgcggcggc
    14521 aggcttctga ccgggctcac ggcgagcgaa atcgacgcgc tcttcgccga cgggacgatt
    14581 cacggcggca tgctgcccaa gatcggctcg gcgctcgacg cggtgagaaa cggcgtcaag
    14641 agctcgcaca tcatcgacgg gcgcgtcgat catgcgctgc tgctcgaggt gctgaccaac
    14701 gagggtgtcg gcacgatgat ccgcgccgac gacgcgccgg cgggcgggac atcgtgatgg
    14761 acgcaccgcg acgcacggcg ccgaagcccg gcgagcggcg cctgcagatc ctgcagacgc
    14821 tcgcgacgat gctcgaagcc ccgcgtggcg agaaggtcac gaccgccgcg ctcgcggcgc
    14881 ggctcgatgt ttccgaagcg gcgctgtacc gccacttcgc gagcaaggcg cagatgttcg
    14941 agggcctgat cgagttcatc gagtcgacgg tgttctcgct cgtcaacaaa atacagtccg
    15001 aatccgagga cggggccgcg caggccgagc agatggtcgt gatgctgctt cgtttcgccg
    15061 agaagaacca ggggatgaca cgcgtgctga tcggcgacgc gctagtcaac gaggacgagc
    15121 gtctgcaggt gcgcatcaac cagctcaccg acaagctcga ggcgacgctg cgccaggcgc
    15181 tgcgcatcgc ccaggcggcc aacggctggc atggcgatgc gaacgcggac gccgcggtgc
    15241 tgatggccta tgtcgtcggc cgctggcaac tgtttgccaa atccggattc aaacgttcac
    15301 cccaggacgg ctggccgctg caacgcaaat tcctgttcgg atgaatgtga cctcccacgt
    15361 cgggcgcgcg ctcggcgccg tccgtccgct tgccactgcg atcgccaccg cgatcgccgc
    15421 gtcgatcgcg ctcgcccagc cgttgccgat gccgccgccg ctgcccgcgc cgcgccaggt
    15481 gacgccggtc ggtcccccgg tcgcgcaacc gccgctgccg tcgccaccgc ccggcacgct
    15541 cggtcagtcc gcgcagggtg ccgaggcgcg cgtgaacgaa ccgccgccgt cgcgctggca
    15601 ggcggcgctg aacgagctcg atgccgattt gcggttgcgc ctgctgacgg ccgacatgcc
    15661 gcccgccgca tggctcgcgg gcgagctcga caccacggat atcgcatcgc aggttcgtca
    15721 ctacgccgcc gcgcggacgt cggcgccgca ggagcggctc taccaggcct cgctcgccac
    15781 cgcatgtctc gtgcgcgtgc agccgcatct tgcagcatgc gaagccgtcg accggctggc
    15841 cgactgggcg cgccgcgata ccggcaacgg cgtaccggcc gtgctgcttg ccgaccgcgc
    15901 gcgacaacgc ggcgaattcg attcggcggc gagctacgtc gaagaggcgg cgagcgcgcc
    15961 ccgcttcgac gattactggt cgctcggcgc actggcctgg tggaactacc tgcgtccgct
    16021 atccctcgcg atcgaccccg cggcgaaagc ggaggcggcg gcgaactatg cgtccgcgcg
    16081 cgatctcgcg tgggcgccgt cgctgcacgg gctctgcatc gaaacgcacg gccgcaccga
    16141 ccgcatgcag gcggcctgcg cgaagctcgg cttggcgatg atgcagcggg gcgccacctt
    16201 tgcgctgcgc cgctcgggcg cgagcatcgc cgaagcgaac gttgccgaag gcacggcgcg
    16261 tgcggccatg cgctcgagcc atgcgcggat catcgccgcg acggcacgct gcgcgcaggc
    16321 gcagcccgat ttcacgacgg cgctcgaatc gccgagtgct gcaacccgct cgcgcggcgt
    16381 cgagcagttc ggcgcctggg cggcggcgca ggcgcgcgac ggcgaagtgg gcgcgtgcga
    16441 gcggctcatt gcgaaacgat aagtggggtc agagacgact tatcgctgaa tcgtctccga
    16501 tcccgtcagg cggccgcaca ccgggcaacc ggggtcgcgc ggcacccgca gttcgcgcca
    16561 ctgcattgtc agcgcgtcga ggagcagcag tcgtccggcc agcgattcgc cgatgccggc
    16621 aagcagcttg agtgcctcgg cggcctgcgt cgcgccgacg atgccgacga ccggcgcgaa
    16681 tacgcccatc gtcgcgcagc gcgtttcctc gagctcgtcg ccctccccga acaggcagtg
    16741 gtagcaggga ctcgagacgt cgcgtgcgtc gaagaccgcg acctgcccgt cgaaacgaat
    16801 cgccgcccct gacaccaggg gtttgcgcgc cgcaacgcac gcgcgattca cgtcgtgacg
    16861 cgtcgcgaaa ttgtcgcagc aatcgacgac gacgtcggct ttggcgacga gggccgccag
    16921 cgcatcgcct cccacgcgcg cagcgatcgg gcgcagctcg acggtcgggt tgatcgcggc
    16981 gaggcggctg gccgccgccg ccactttctg ctgcccgatg tccgccgtcg catagagaat
    17041 ctgccgctgc aggttcgtga gatcgacctg gtcggcgtca cagagcgtca tcgtgccgac
    17101 gccggcggcc gcgaggaact gcgcgaccgg cgcgccgaga ccgccgatac cgatcacgag
    17161 cgcgtgcgcc gccgcgaatt tgtcttgcgc atccggtccg agctcgtcga gcaggatgtg
    17221 ccggctatag cggaggagtt gctggtcgtc cacgacaggt aacaggtgac aggagacagg
    17281 caacagtatc aggccgcgcc agtgcctcct gtcacgtaac gcgttgcaag cgacagaaac
    17341 ggcgccgccg gcgcaaaggc cggcggcgtc tgtgacctgt taccgacttc cgttacttgg
    17401 cagccgccga ctgcagcttc accgagtcga tcaccggcag gcccttcaga tgattgacgg
    17461 cctgcttcaa ctggaagtcg tcgtccgatc cgaagtcgag ccgcggcggc agcgcgtcga
    17521 tcttgccacc ggtcgccggt accggcttca cttccggtat cgcagcggcc gcatccttgg
    17581 cgcccggctt gcccgtcgtg ctgacgtcgc ccggcacgtc gaggtgatgc tcgaggttcg
    17641 cctcgcggat gcgattcggc gagtcgcggc catcatcgac gaggaagtcg ggcacgatgc
    17701 ccttcgcctg gatcgagcgg ccggccggcg tgtagtagcg cgccgtggtg agcttgagcc
    17761 cggtgttgtt gccgagaggc aggatcgtct gcaccgagcc cttgccgaac gtctgcgcgc
    17821 cgagcacgac ggcgcgcttg tgatcctgca gcgcgcccgc gacgatctcc gacgccgatg
    17881 ccgttccgcc gtcgacgatg acgaccatgg ggacggtctt gatgccggcc ggcaggtcgc
    17941 gcaggtaatc atcgccgcgc cgaacgtagt tttcgcgatt cgcggtgagg cgcatgtgcg
    18001 catcgggcgt gcggccgtcg gtgtagacga ccagcgcgtc cttcggcagg aacgccgacg
    18061 acaccgccac cgcctggttc agcaggccgc ccggatcgct gcgaagatcg agcacgagtc
    18121 ccttgagcgg gcccttcgcg tacaggtcct tgatcgcctt ggcgagatcg ctgcccgtgt
    18181 cctcctggaa cttgcgcacg cggatgaagc cgtagcccgg ctcgagcatc ttcgcgcgca
    18241 cgctcttgac gttgatttcc tggcgcagga tcgtgaacgt cagcggcgtg tcgacatcct
    18301 tgcgcacgac ggtcagaagc accggcgtgc cgggcgcgcc gcgcatcttc tcgaccgcct
    18361 tggacagcgg catgccgcgc gtggccgtgt cgtcgatctt gacgatcagg tcgcccgact
    18421 tgatgccggc cttgaacgcg ggcgtgtcct cgatcggcga aacgacgcgg atcacgccgt
    18481 cctcgacgcc gacctcgatg ccgaggccgc cgaacttgcc ctgcgtgtcg acctgcagct
    18541 catggaaggc ctcggcgtcg agaaagtccg aatgcgggtc gagcccgtgg accatcccgt
    18601 tgatcgcttc cttgatcagc ttgtcgtccg acaccggctc gacgtagtcg gacttgatct
    18661 tcccgaacac ggccgagagc aactgcaggt cctcgtacgg gatcggcagc cgcgtttcgc
    18721 gctgcgcgaa caccgaaagg ttcaggctca acagcaaccc gagaactgca cccatgccaa
    18781 tccagcctgc cttcttcatg cgttcgcgca tcgctgcctc tgtttcatcc ttgtcggccc
    18841 gccgctcgcc gaggctcgac gcctcgaaaa agcccggaat ttcatccagc aaaaagtata
    18901 cgcctgcacc cgtccgcaaa cgcaaccgcg cccgccgggc acacgcgccg cggcgccagt
    18961 gcagctgcca ctgtcgtcca cgggcgtgtt tttgctgttc ttgggagccg gccggcggtc
    19021 gggagccgat cggtggatga ccgctcggcg ctaggcggcg ctacgtcccg gtgatctcgt
    19081 ggacgagcgc gtcggcttgg tccttcgcgt ccggctgaag ttccgcgggc ggcggaatta
    19141 tagttcagaa gcgcagcagc gaatgcccgg tcatctgcgg cggcttcggc agccccatca
    19201 cggcgaggag cgtcggcgcg acgtcctgca gcgcgccgcc gtcgctcatc atgctcgccg
    19261 ggcgtccgac gtagacgaac ggcacgaggt ttcgcgtgtg tgccgtgtgc gcctggccgg
    19321 tggtctcgtc gtgcatccgt tcggcgttcc cgtggtccga cgtgatgatg acgtcggtcc
    19381 cgtgcgcacg cgccgcgtcg accacgcgcc cgatgcaggc gtcgagcgtt tcgatcgccc
    19441 gcttcgccgc ctcgaagttc cccgtgtgtc cgaccatgtc ggcgttcgcg taattgcaga
    19501 tgatcgcgtc gtagcgctcg ccctcgatcg cctcgacgag ccggtcggtc acttcgggcg
    19561 cgctcatctc gggcttctgg tcgtaggtgg cgaccctcgg cgacgcaacg aggacgcgat
    19621 cctcgcccgg atacaccgcc tcgctgcccc cgttgaagaa atacgtcacg tgcgcgtact
    19681 tctcggtctc cgcgatgcgc aactgggcaa gatgcaaccg cgcgaggtac tcgccgaagc
    19741 tgttctcgat cacctgcggc gcgaacgcaa cgggcaggcc cgcgaaatcg tcgccgtagt
    19801 tcgtgaggca gacgaagcgc gacagcttcg ggacgcgcgg ccggtcgaac gcgtcgaagc
    19861 cgggatcggt cagcgcgcgc gtcaattcgc gggcacggtc ggcgcggaag ttcatgaaca
    19921 cggcgacgtc gccgtcgtcc atgcgcgtcg tgcgtccctg cgcatcgccg atcgccgtgg
    19981 cctgcacgaa ctcgtcgctt tcgccgcgcg cgtacgccgc ctcgagcgcc gcccgggcgc
    20041 tttccgcgac gtgcgccgcc cggccgtcgg cgacgagcgc ataggccggg gcgacgcgct
    20101 cccagcgctt gtcgcggtcc atcgcgtagt agcgcccgca gatcgacgcg atgcgcgcat
    20161 gcggatggcg cctgcacacg tgctccatcg cgtcgagcga tgcgcccgcg ctgcgcggtg
    20221 gcgtgtcgcg gccgtcgaga aacgcgtgca cggcgatgcg cggggcaccg ccggttgccg
    20281 ccatctccac catcgcctgg atctgacgtt cgtggctgtg cacgccgccg ggggacagca
    20341 ggccgaggac gtgcagtgtc gcaccgcccg cgcgcgccgt ttcgaccgcg tcggcgagaa
    20401 ccggattgcg cgcgaattgt ccgtcgcgga tcgcgacgtc gatgcgggtg aagtcctggt
    20461 agacgatgcg gccggcgccg atattgagat ggccgacctc ggagttgccc atctggcctt
    20521 ccggaagacc gacgcgcagc tcggacgcgt cgatcgtcgt gtgcgggcac gtcgcaagca
    20581 actgccgcca attcggcgcg tcggcgcgcg cgattgcatt atcgggcgcg tcggcgcgac
    20641 agccgaagcc gtcgaggatc agcagcacga cgcgatgcac tgcacgcagc gccggttgcg
    20701 gttcgacgac agcggaaggg gagggcggga ccttgacgtc catcgggtga cgcattggtg
    20761 cggcgcgccg ggatcgtgac gcaccgtaag ctatcatttt agactttgtc cgcgggagcc
    20821 tcgttctgtt gcccatcatc gaagggttcg tgatcaggaa ctggccgctc gtgctggttt
    20881 tcgtgatctc cggcgcgctg ttgctgtggc cgctcgtgca gcggcgcctt gcgccggggc
    20941 gcgagatcag cacgctcgac gcgacgaggc tgctcaacaa ggagcagccg ctggtgctcg
    21001 acgtgcgcga ggcgaacgag ctcgccggcg gaaagctgcc gaacgccacc catatcccgt
    21061 tgtcccagct caaggatcgc ggcggcgaga tcgcgaagca tgcggcgcgt cccgtgatcg
    21121 tctattgcga tcgcggcgcg cgtgcaggcg ccgcggtcgc agcgctgacg cgcctcggct
    21181 tcacgcacgt tcagtcgctt cgcggcggct ttcgtgcgtg gaaggacgcc gggctgccgc
    21241 tttcgaaatg aaaatcggac gcagacgatg aggccgcaag tgacgatgta caccagcgcc
    21301 tattgcggct actgccgccg cgccgagcgg ctgctcgaga cgaagggtgt caccggcatc
    21361 gcgatgattc gcgtcgacga gcagcccgag gcgcgcatgg cgatgatcga gcgcaccgga
    21421 cggcggaccg ttccgcaaat ctacatcggc gaccaccatg tcggcggcta cgacgagctc
    21481 gcggcgctcg atcgcgcggg gcggctcaac gggcttcttg gcggcgaagc cgcctgatcg
    21541 cgcacgcttc gtagcgtaga atcgcccgct tttcctttcg gagcattcga gcatggccga
    21601 ctcgcccgcc acgaacgcgg attcgcaacc cgcagccgcg cccgctggcg tcagcgcgca
    21661 gttctcgatc gagaagatct acgtcaagga cctgtcgctg gagaatcccg gcgcgccgca
    21721 gtcgttccag atgaccgagg cgccgcagat cgagatcggc cttcgcaccc gtgccgagca
    21781 ggtggcgcag gatacctacg aatgcgtgct gacgatcacc gtcaccgcgc gctcgggcga
    21841 caagacggtg ttcctggtcg aggcggcgca ggcgggcctt ttcgccattc gcggcgtgcc
    21901 ggccaatcag ctgcagccgg tgctggcgat ccactgtccc gcggtcctgt tcccgtacgc
    21961 ccgcgagacc gtggcgagcg ccacgatcca tgcgggcttc ccgccggtgc atcttgcccc
    22021 gatcaatttc gacgcgctct accagcagca actcgcgcag gcggcggccg gcgcgcccgc
    22081 taccgcgaat tgatccgtac cggcgttccg tacatcgcgg cgcgcgcgct tgccatcgcg
    22141 ctgccgctgc tggccggcgt tgcgagcgcg gccgatttcc gatctacgcg cgacgcgtcg
    22201 accgttctct acgatgcgcc gtcggcacgc gcgaagccgc tcttcgtgta cgggcgcgac
    22261 gttcccgtcg aatcgctggt gagcgtcgaa ggctggacga aggtccgcga tgcgtcgggg
    22321 acgatcggct ggatgccgag caagtcgctc gccgacaagc ggatgctgat cgtgcgtgcc
    22381 cccggggccg acgtgcgcgc ggcgcccgac gacaaggcag cggtggtgtt tcgcgccgag
    22441 cccgacgttc tgctcgaact cgccgagccc gcggcatcgc cggcggcgac ggcgacgccc
    22501 ggatgggtca aggtgcgcca ccgcgacgga caaaccggct acgcgaggct cgcgcaggtc
    22561 ttcggcctct gaccgcatgg ccttcccaat gagcgcgaac gtcgcggtgc tgggcggcgg
    22621 cgcttgggga acggcgtttg ccgtccacct cgcggcacgg acgcatgcac catcgcgcgt
    22681 cacgctgtac atgcgcgacc ccgcgcaggc gctcgccgtg cacgccgcgc gcgagaatcg
    22741 ccgctatctg cgcggtgttc gcctgccgga tgcgctcgcg gtcgtgtccg atctcgcggg
    22801 cattcgtgcc gacgtcctgt tcgcggccac gccggtcgca gcccttgcgc cgcttgccgg
    22861 cgcactcgcg gatgcgggcg tgcatgcacc gctcgtctcg ctcgcgaagg gcttcgtcgc
    22921 cgcagccgag ccgggcgggt ggacgttgcc gcaccgcatg cttgcgtcgc gctggccgca
    22981 cccggtcggt gtcgtgtcgg gaccgacgtt cgcgaccgaa gtcgcccagg gactgccgag
    23041 tgccgtcgtg gttgcaacca acacggcgcg gctgaccgac gacgtcgcgc aactgctgcg
    23101 cggcgaaacg atgcgcgcgt atccaagcga tgacgtgacc ggtgtcgaag tcggcggcgc
    23161 ggtcaagaac gtgctcgcga ttgccgccgg cgcgagcgat ggcctcggct tcggcgacaa
    23221 cgcgcgcgcg gcgttgatca cgcgcgggct cgccgaggcg gggcggctgt cggcagcact
    23281 cggcgggcgt ccgcagacgc tgaccgggct cgcgggcctc ggcgacctcg tcctcacgtg
    23341 caccggcaac ctgtcgcgca atcgccgcgt cgggcttgcg ttgtcgcgcg gccagccgct
    23401 cgcggaaatc ctcgcggagc ttcaccatgt cgcggaaggc gttgccgcaa cgcgtgccgc
    23461 gcgcgagctt gcactgcatc atggcgtcga catgccgatc gtcgacgccg tgcatcgcgt
    23521 gctttatgga cacgtcgcgc cgcgcgacgc cgtgacggcg ttgcttgcgc gcgagccacg
    23581 cggggaatga ctgcgttcgc cacgaaagga aggtcccgat gcgcgtcgcg ctgggctttg
    23641 cgatgaccct tgcaatctcg gccggtgcgg caggcgccgc ttcgcctgcg ttttttgcac
    23701 ccgagacgcc cgcgcgaccg atggtcgacg tgctgcacgg cacgacgctg accgatcgct
    23761 accgctggct cgagaacggc aaggacccgg aagttgccgc gtggacacgc gcgcagcacg
    23821 cggcgagcgt gcaatatctc gatcgcaacg caccgccggt gccgggcatg gaggacgagc
    23881 tcgcgaagta cttcgaccgc gatcgcaccg atccgccgct cgtcaagcac gggcgccagt
    23941 tcttccagcg cacccggcgc ggcgagccgc aggcgaagct ctacacacgc atcgatggac
    24001 gcgacgtgct gctgttcgat cccgttgcgc tcgatccgtc gggcaggacg aagatcggcg
    24061 acgtcgtgcc caaccgcgac gcgacgaagc tcgccgtcgg cgtctatgcg cagggcaccg
    24121 agcgcaagga ctaccggatc atcgacagca cgaccggtgc acagaccggc cccgcgatca
    24181 gcggtctcga cagcttcggc tgggcgcgcg acgagcggtt cgcgttcatc acgccgcgca
    24241 ccgcggagtc cgacgcgaag caggagccga tgcggtgctt gcgccatcgg ctcggcggcg
    24301 atcgccggga cgacgtgctg ctgatgaagg tcgacgacgc aaagaattac tgctcggtgt
    24361 acgagcccga ggatgcggac gtcaccgtct tcgagaacgg cgacttctgg tcgaacaccc
    24421 tccgcatccg gccgatcggc tcggacgccg agccgcggac gatctactcg agccggcagt
    24481 ttcgcgccga ggcgatcttc cgtcgcgacc ggatgtattt ccgcaccaac gaccatgcgc
    24541 ccaactggaa gctcatggcg gcgagctacg cgaagccgca atccggcgac tggtcgacgc
    24601 tcatcgccga gcaggcgaac gtgctcgccg acgcgacggt cacgagcgac gtcatcgtcg
    24661 cgacgctgcg cgaggacgtg ctctcgaggc tcgacgtgta cgaccacgcc ggcaagcgcc
    24721 tgcgcgagct tccgctcccc gagttcggca atgtctcggg cattgcgtac gacctcgacg
    24781 ccgaccgggc gtacgcgacg ctcgcgtcgt acacggcgcc gtccacgctt tacggcctcg
    24841 acggcaagac gctcgcgtgg gccctcgtgt ggcaggacga cccgccgctg gacctgtcgc
    24901 aggtcgccgc ggaacgcgtg tacgtttccg ctcgcgacgg cgcgcgcatc cccgtattcg
    24961 tcgtgcaccg gaaggacatg gcaaaaaacg gcgtcaatcc cacgctgctg cacgtctacg
    25021 gcggcttcga caatgccgtc gagccgttct acctcggcag ctacgcgtcg ttcgtcaacc
    25081 gcggcggcat cttcgtcgat gccggcgtgc gcggcggcag cgaatacggc gagaagtggc
    25141 acgagcaggc gatgctcgcg aacaagcaga acacgttcga cgacacgatc gcggtcgccg
    25201 aatggctggt gcacgagggc tacacgaagc ccgcgaagtt ggcactcgag ggaggcagca
    25261 acggtggcct gaccgtcggc gcggtgatca cgcagcggcc cgacctgttt gcggcggcga
    25321 tctgccaggt gccgctgctc gacatggttc gctatcacag attcctgatc gcgcgctact
    25381 ggattcccga gtatggcgac ccggacaagg acgccgattt tcgctggatc ctccgctatt
    25441 cgccgtacca gaacgtgcgc gccggcgtga acctgccgca gacgctcgtg gtggcaggcg
    25501 aatacgattc gcgcgtcgac ccgctgcacg cgaagaaatt cgtcgccgag gtgcagaacc
    25561 atccggggca ggtgtcgccg ttcctgctgt acatggactt cgacagcgga cacggcacgg
    25621 gcaagacgca gcagcagcgc gtgatcgacc gtgattacga gctgcgcttt ctgatgagcg
    25681 cgctgtcgat gaagcaggac gacacaccga agtagggtca gagacgactt tcggaaaaca
    25741 ggatcggcga gcggtgacgc tcgccgaaag tcgtctctga ccctatttga agccgttctg
    25801 ccgccacgcc tcgtacacgg ccacggcgac cgcgttcgac agattgaggc ttcgcacgcg
    25861 cggccgcatc gggatgcgca cgcgcgcgtc ggccgcgaac tgcgccacga tgccggcggg
    25921 cagtccttgc gactcgcatc cgaacgtcag gacgtcgccg ggcgcgtacg cgacgtcgta
    25981 ggacgaacgg cgtgcctgcg tcgtgaacgc gagccagcgc cgcggcccgt cggcatcgag
    26041 cgccgcgcgg cacgcggcga aatcgcgatg cacgcgcacg ctcgcatact cgcggtagtc
    26101 gagcccggcg cggcgaagct cgcggtcgtc catgcgaaac ccgagcggct cgacgagatg
    26161 cagcccgacg ccggcattcg cggtgaggcg aatcacgttg ccggtgttcg gcggtatttc
    26221 gggatggacg agtacgacgg cgaacatgtt cgggcctcac ggcggcgggg ttcgagcgac
    26281 gacccacgcc tcgacgcgga tcgcgcctgc cttgcgcacc gcgatggcag ctgcagcaag
    26341 cgttgcgccc gtggtcatca cgtcgtcgac gatcgcgacc gcgcggccgg caacgcgctc
    26401 gttcgcctcg aacgcattgc ggacgttgcg cgtgcgctcg cgcagcgtca agcccgcct
//
LOCUS       DASSVX010000254         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1661193, whole genome shotgun sequence.
ACCESSION   DASSVX010000254 DASSVX010000000
VERSION     DASSVX010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661193"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1596)
                     /locus_tag="VFO53_10960"
     CDS             complement(88..1596)
                     /locus_tag="VFO53_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET8876838.1"
                     /translation="MIPLDSHLNDALLFAAYALALVLIAVLLRSPPRRAMLGMLAVIA
                     IGVLGLWVYEGRASAMKTATSSVMVREAVLVLIAFGVIQITVLFIFQTVLVRRRLPRI
                     LNEFVVALSLIGYAIYRLNAVGVNLAGLITTSAVVSGALAFSAKETLGNLWGGIAIQL
                     EKTCRIGDWVRIDTVTGQIVSIRWRYMAIATINNETIVIPNGNVMQHRLTVIGRRGEE
                     RTPWRRYVSFDVELQHPPSRVIAEVERELAHAQIPFVSQQPHPSVGCIDFKDSGIEYA
                     VAYDLIDTAQYWTVDSLVRVHVYAALMRQGLDIPFPRRIIEVRQDERAQATQREVDHR
                     VAALVTIDLFSSLTDAERAALAQKLMTCAYVRDEIVFRAGDAADSLYLLAKGRVDIVS
                     EPADGKRAKLATLVAPAYFGEMGLLLGQPRFATVVAVDEAICYRLDKDGFDAVIQARP
                     ALAERLAQVLAERQAANDATLQALDAEVLAQRGANRKSDLLRRIQQFFGLAQ"
     gene            1692..>3299
                     /locus_tag="VFO53_10965"
     CDS             1692..>3299
                     /locus_tag="VFO53_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET8876839.1"
                     /translation="MGIRSGGQILVANLLAQRATLAFGVPGESYLAVLDALHGAAGRL
                     RFIVCRHEGGAAYMAEAYGKLTGRPGVLFVTRGPGASNAAVGIHTAMQDSTPLIAFVG
                     QVGTGMVDREAFQEIDYRRMYGSVAKWVAQIDRAERIPEYVAHAYRVAMSGRPGPVVL
                     ALPEDMLAADADVADAPYVEAIAAAAPRDDVRRVEAMLRDAHRPLVIAGGSRWDDAAR
                     GALVRFAEASQLPIACAFRRQDLFDNAHPLYAGDVGIGINPKLATRVREADVLLVIGE
                     RLGEMTTSGYTLLDVPVPKQTLVHVHPDASELGRVYQPALAIAATPGAFLAAIDERAV
                     VPVAQRDAATPAHADYDAWRARRPVPGDVDLWQVVQWLDERLPADAVLTNGAGNYTAW
                     VHRLFRYGGERRQLAPYSGSMGYGVPSAVAAKLVHPGRAVVSWNGDGCFLMNGQELAT
                     AVQYGLAIVFVVIDNGMYGTIRMHQEREYPGRVVGTDLVNPDFAQLARAYGAHGETVV
                     TTDAFAPAFERALAAKRPALLHVKIDPQ"
BASE COUNT          547 a         1095 c         1140 g          517 t
ORIGIN      
        1 gggcgttagt ggcctgtcac gggtcacgca cgttcacaac cgattccccc gtcacgggcc
       61 accagccggc cgcgccggcc tgcgcgatca ttgcgcaagg ccgaagaact gctggatcct
      121 gcgcagcagg tcactcttgc gattcgcgcc gcgttgcgcg agcacttccg cgtcgagcgc
      181 ctgcagcgtg gcgtcgttcg ccgcctgccg ttcggcgagc acctgcgcga gccgctcggc
      241 caacgcgggc cgcgcctgga tcaccgcgtc gaagccgtcc ttgtccagcc gatagcagat
      301 cgcctcgtcg acggccacca cggttgcgaa acggggctgc ccgagcagaa gtcccatttc
      361 cccaaagtag gcaggcgcaa ccagcgtcgc gagcttggcg cgttttccgt cggccggctc
      421 ggacacgatg tcgacccgtc ccttcgcgag caggtacagc gagtcggccg cgtcgcccgc
      481 gcggaagacg atctcgtcgc gcacgtaggc gcaggtcatc agcttctgcg cgagtgcggc
      541 acgttcggcg tcggtaagcg aggagaacag gtcgatcgtg accagcgccg cgacgcggtg
      601 gtcgacttca cgctgcgtcg cctgcgcacg ctcgtcctgg cggacttcga tgatgcgccg
      661 gggaaacgga atgtccagcc cctggcgcat caatgccgcg tacacgtgga cacggacgag
      721 cgaatcgacg gtccagtatt gcgcggtatc gatcaggtcg taggcgaccg cgtattcgat
      781 gccgctgtcc ttgaaatcga tgcagccgac cgatggatgg ggctgctgcg agacgaatgg
      841 aatctgcgcg tgcgcgagct cgcgctcgac ctcggcgatg acgcgcgacg gcggatgctg
      901 cagctcgacg tcgaacgaaa cgtaacgccg ccacggggtg cgctcttcgc cccggcggcc
      961 gatgaccgtc aaccggtgct gcatcacgtt gccgttggga atgacgatcg tctcgttgtt
     1021 gatcgtcgct atcgccatgt agcgccagcg aatgctcacg atctggccgg tgacggtgtc
     1081 gatgcgcacc cagtcgccga tgcggcaggt cttctcgagc tggatcgcga tgccgcccca
     1141 caggttgccg agcgtctcct tcgccgagaa cgcgagcgcg ccggaaacca cggccgacgt
     1201 cgtgatcagt ccggcgagat tgacgccgac cgcgttcaac cggtagatcg cgtagccgat
     1261 cagcgacagg gccacgacga actcgttcag gatgcgcgga aggcgccttc gcacgagcac
     1321 ggtctggaag atgaacagta ccgtgatctg gatcacgccg aatgcgatga gcacgagcac
     1381 cgcttcgcgc accatgaccg acgacgtggc ggtcttcatc gcgctcgcgc gcccctcgta
     1441 cacccaaagc ccgaggacgc cgatggcaat cacggcgagc atgccgagca ttgccctgcg
     1501 cggcggcgag cgcagcagca cggcgatcag cacgagcgcg agcgcatacg cggcaaacag
     1561 gagcgcgtcg ttcaggtgcg aatcgagcgg gatcacgggg tgcgccggag acgaaacgcc
     1621 gcgattatag gatgtgcccg cgtaaaatcg gccgcggcgt cgagagccgc catggaagga
     1681 gtaacgaacc gatgggcatc agaagcggag gacagatcct cgttgccaat ctgttggcgc
     1741 agcgggcgac gctcgcattc ggcgtgcccg gcgaaagcta tctggcggtg ctcgacgcgc
     1801 tgcacggcgc ggccggtcgg ctgcgcttca tcgtctgccg tcacgagggc ggcgcggcgt
     1861 acatggccga agcctacggc aagctgactg gccggccggg cgtcctgttc gtgacccggg
     1921 gtcccggcgc gtcgaatgcc gccgtcggca tccatacggc gatgcaggat tcgacgccgc
     1981 tgatcgcatt cgtcggtcag gtgggcaccg gcatggtcga ccgcgaggcg ttccaggaga
     2041 tcgactaccg gcgcatgtac ggcagcgtcg cgaagtgggt ggcacagatc gatcgcgccg
     2101 aacgcatccc ggaatacgtc gcgcatgcgt atcgcgtcgc gatgtcggga cgtccgggcc
     2161 ccgtggtgct ggcacttccg gaagacatgc tcgcggccga cgccgacgtc gccgatgcgc
     2221 cgtacgtcga agcgatcgcg gcggcggcac cgcgcgacga cgttcgccgt gtcgaggcga
     2281 tgttgcgcga tgcgcaccgc ccgctcgtca tcgccggcgg cagccgctgg gacgatgctg
     2341 cgcgcggcgc gctcgtgcgc tttgccgaag cgtcgcagct tccgatcgca tgcgcgttcc
     2401 gtcggcagga cctgttcgac aacgcgcatc cgctgtatgc gggcgacgtc ggcatcggca
     2461 tcaatccgaa gctcgccacg cgcgtgcgcg aggccgacgt gctgctggtg atcggcgagc
     2521 ggctcggcga aatgacgaca tcgggctaca cgctgctcga cgttccggtg ccgaagcaga
     2581 cgctcgtcca cgtgcatccg gacgcgtcgg agctcggccg cgtctaccag ccggcgctcg
     2641 cgatcgccgc gacgccaggc gcatttctcg ctgcgatcga cgagcgcgcc gtcgtgccgg
     2701 tggcgcaacg cgacgcggcg acgcctgcgc acgccgacta cgacgcatgg cgtgcgcggc
     2761 gaccggtgcc gggcgacgtc gacctgtggc aggtcgtgca gtggctcgac gaacgcctgc
     2821 cggccgacgc ggtgctgacc aacggcgcgg gcaactacac cgcgtgggtg cacaggctct
     2881 tccgctatgg cggtgagcgg cgacagctcg caccgtactc gggatcgatg ggctacggcg
     2941 tgccgtcggc cgtcgcggcg aagctcgtcc atcccgggcg cgcggtcgtc tcgtggaacg
     3001 gcgacggttg cttcctgatg aacgggcagg agctggcaac ggccgtgcag tacggccttg
     3061 ccatcgtctt cgtcgtcatc gataacggca tgtacgggac gatccgcatg catcaggagc
     3121 gcgaatatcc gggacgcgtc gtgggaacgg atctcgtcaa tcccgatttc gcgcaactcg
     3181 cacgggcgta cggcgcgcac ggcgagacgg tggtcaccac ggacgcattc gcgcctgcgt
     3241 tcgagcgcgc gctagcggcg aagcgaccgg cactcctcca cgtcaagatc gatccgcag
//
LOCUS       DASSVX010000255         9424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_628088, whole genome shotgun sequence.
ACCESSION   DASSVX010000255 DASSVX010000000
VERSION     DASSVX010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628088"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VFO53_10970"
     CDS             <1..899
                     /locus_tag="VFO53_10970"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET8876840.1"
                     /translation="GRGRCALGESPVWNERDGRLCFVDLRDTPAIHALDVASGDEARW
                     PMPEDIGCIAPCTDGTLIAGLRSGFATFDLARGALDYFFDPEPDKHANRLNDGKCDAR
                     GRLWCGSMNPDSALAEGSLYVLERTSCRRVLDGWLTPNGIAWSLDGATMYVADTRRGV
                     IDAFAFDRDDGSLHGRRVFADLAALPGGPDGACVDRDGFLWSAIFDGGCLLRLDPSGR
                     IDRVLRLPVSKPTSCAFGGADYRTLFVTTASRGLSASRLAAEPAAGHVLALDVGAAGV
                     APDAFIPASAATRSASRGFEHG"
     gene            892..2061
                     /locus_tag="VFO53_10975"
     CDS             892..2061
                     /locus_tag="VFO53_10975"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HET8876841.1"
                     /translation="MASDSQRFTGYRRRDGSVGVRNHVLVLSPTGLTSAAAQRIADLV
                     LGTVAVTSGFGRGQVGDDAQLHFDTLAGLARNPNVAAVLVVSAADAITESYVDALRES
                     GKPVDGLSLPGVQEDALTLVDRGVRIATRLVREASQARRESSTLTDLFLAVECGHSDA
                     TSGLVCNPLTGRLAERIVGAGGRAVFSETVEWTGTEHLLARRAATPDVAQRIIATVMA
                     REHAVRESGGDIRAQNPGPQNRTGGLTTIEEKALGAIAKGGEQPIQGVLRAAERPSQS
                     GLYLMDTPYFSPESMTAMVAAGAQMVVFTTGAGNSYCSALAPTFKMSANPDACARLRE
                     QIDFAATGVLDGSMTFDDAAERALDALLGVASGTLTYGEIVGEGAEVVSRLAPSI"
     gene            complement(2130..3734)
                     /locus_tag="VFO53_10980"
     CDS             complement(2130..3734)
                     /locus_tag="VFO53_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET8876842.1"
                     /translation="MADVDVLIAGAGPTGLVLALWLAQSGVRIRIVDKAAEPGTTSRA
                     LVVHARTLEFYRQLGIANAVVDAAVRFAAVNLWVAGKQAAHVDLGALGEGATAFAFML
                     IFPQDEHERLLIERLSAAGVRVERPCELRGFDERVDRIVAHLVHGDGREERCEAAFLA
                     GCDGAHSTVREKLQAGFPGGTYAHVFYVADVQASGPVMNHELHVALDEADLLAVFPMK
                     GDARARLIGTVKEGGSETTFHRGAVQTAKAPEVVSGPPSALEWRDVSHTVLQRLGIDV
                     TQVNWFSTYHVHHRVASHFRSGRAFLLGDAAHIHSPVGGQGMNTGIGDSANLGWKLAA
                     AAASRIDARVLDTYEPERIAFARQLVATTDRAFQLATSDGAIARFVRIRVLPRVLPQL
                     LRLRVLRRFMFRTLSQTAIRYRESALSRGSAGSVHAGDRLPWVGHADGTQRVADNYAA
                     LSSLDWQVHVYGQAPGGVEQACEHRSVALHAFAWQASMQRAGLARDAVYLVRPDGYVA
                     FADATGDATALARYLDDWRIMTRAAA"
     gene            3818..4828
                     /locus_tag="VFO53_10985"
     CDS             3818..4828
                     /locus_tag="VFO53_10985"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HET8876843.1"
                     /translation="MANIVTPVRQLVMADSTHWAFPPEMQPKSDDVGFDLTYALNAVV
                     AMRVDVVEDAFTASVLGTERTGNGVVISDDGIVLTIGYLITEADSIWLTTNGGNAVPG
                     HALAYDQVTGLGLVMPLGSLGVAPLPRVDLASADLDDDVYVIGHGGRAHALRAKVFSR
                     REFAGYWEYLLDEALFTTPAHPEWSGAALLDGRGRLVGIGSLFVQEADGDQSVKGNMF
                     VPSELIDPIQDDLLRTGRADRAPRPWLGIYTTETKDGIVVQGLADDGPAARAGVELGD
                     LVRAVGDEPVERLAGFYRSVWQRGAAGVEVPLTLARDDKLWNVTVQSVDRSELLKKPL
                     LQ"
     gene            complement(4773..6338)
                     /locus_tag="VFO53_10990"
     CDS             complement(4773..6338)
                     /locus_tag="VFO53_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET8876844.1"
                     /translation="MSHFPGAHRSLRFALAVAAVSFALAACTTTPTAPPAVELPPPTI
                     DHLELSHWWTEFNEPALTALVDEALANNLDLQAAIARVETARAQVTLASAALYPSLNL
                     GANAGRSRVTEVGTNPLPPGFGATGNDFRLGLQASYEIDLWGKYRTATRAAQNDLLAT
                     EYARESVRTVVAATVAQTYFGLMAADAQLQLLKDTLALREQTVALQTDRAQAGVIGEY
                     DLAQAKAERDAVVADIATAQRSVSQFESGLAVLTGRSPRDVFAPQVARETTLAKLLAV
                     PTVPAGLPSDVLERRPDVREVEKELVAASLRIDRARADYFPSVTLTGELGTESGALRH
                     LASGPALIWSLGAGLVQPLLNLKAIEANVAAQQARRQELVVNYRQTVLAAFKDVHDAL
                     AANESTRAALAAQMTRSGNLRQAYDLSDLRYRAGYSPYLEVLDAQRQLLQAQTLSILA
                     ARDVRLTLVDLAKALGGGWDYQNAVEPAAGDASAQKPAASREERAPGTANNGALTAAE
                     ASSKARYDRPTAR"
     gene            complement(6340..>9424)
                     /locus_tag="VFO53_10995"
     CDS             complement(6340..>9424)
                     /locus_tag="VFO53_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008219380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HET8876845.1"
                     /translation="PEIAPPVVTVQAVYPGASAQTLEQTVASPIENSINGVPGMIYMS
                     SSSASNGIVQIQVTFDIGTNVDIAAVNVNNRVNQVLPRLPEEVRRQGVTVERGSSAFL
                     QVLAFYSPDGRYNDLFTSNYVNLNVLDALKRLPGTTNVQIFGAHDYAMRIWLKPDRLA
                     QLRLTPGDVIAAVNEQNAQFAAGKVGQAPSPSNQSLVYTITTKGRLTDANEFSQIIVR
                     ANPDGSTVRLGDVARVELGSKDYEFIGRFNGKDATLVGIFLSPGANALDVAKSVRAEV
                     ATLATRFPEGLSYAVPYDTTRFVEVSIREVLKTLGEAMLLVFLVVYLFLQSWRAAIIP
                     FAAVPVSLIGAFAGIYMLGYSINTLTLFAMVLAIGIVVDDAIVVLENVERHMREDHLP
                     AREAALKAMREVTGPIIAIVLTLTAVFVPIAFLGGLTGELYRQFAVTIAMSVVISGFV
                     ALTLSPALSALILKPHQRPNRFFRWFNDWFARVTGRYNDGVIFLLRHGILSMVLFLSM
                     VAVTAWLWRTTPGSLVPDEDQGFYISAVILPDGATLERTDKVVSEVSAAIRSNPANQD
                     IVAFTGFDFIGGGFRNNAATIFVTQKPWDQRKVTAPQLVGELFGKTAGIKEALVLAFN
                     PPPIFGLGTAGGFEFYIQNHGEGGAKRLDEVKNAFLAKANSDPVLAGAQTLWRATVPQ
                     LNVDVDREKAKTLGIPISDIFAALSATLGNFYINDFNKYGRAWQVLMSAEPSYRNRPD
                     DIGAVYVRSAKGEMVPLSSLVTVRYTSGPDALDRFNNLPAVKIIGQAAPGYSSGQAIA
                     EIEKIAGEVLPSDFSYDWGGTSYQEKKSSGAATFSLGLAVIMVFLILAAQYERLSLPL
                     SVLLALPFGTFGALAAIWLRGLTNDVYFQIGLVTLLGLAAKNAILIVEFAVLKTHEGM
                     TPSAAAIEAARLRFRPILMTSLAFILGVTPLALSTGAGAGARHSTGTGVMGGMLAATF
                     LAVFFVPLFYRVIAERRLIERRSHDELLGEVKHARAATHHVPHTPGHPPVPAHGD"
BASE COUNT         1520 a         3173 c         3230 g         1501 t
ORIGIN      
        1 tggggcgcgg tcgctgtgcg ctgggcgaga gtcccgtgtg gaacgaacgc gacggccggc
       61 tttgtttcgt cgatcttcgc gacacgccgg cgatccatgc gctcgacgtc gcgagcggcg
      121 acgaggcgcg ctggccgatg cccgaagata tcggctgcat cgcgccgtgc accgacggca
      181 cgctgatcgc gggcctgcga tccggcttcg cgacgttcga tctcgcgcgc ggagcgctcg
      241 attacttttt cgacccggaa ccggacaagc acgccaaccg attgaacgac ggcaagtgcg
      301 atgcgcgcgg gcgactgtgg tgcggctcga tgaatcccga ctcggcgctc gccgagggaa
      361 gtctctatgt gctcgagcgg acgagttgca ggcgcgtgct cgacggttgg ctcacgccga
      421 acggcatcgc atggagcctc gacggcgcga cgatgtatgt cgccgacacg cgccgcggcg
      481 tgatcgatgc gttcgctttc gatcgcgatg acggctcgct gcacgggcgc cgcgtgttcg
      541 ccgacctcgc cgcgctgccc ggcgggccgg acggcgcctg cgtcgaccgc gacgggttcc
      601 tgtggtcggc gatcttcgac ggcggctgcc tgctgcgtct cgatccgtcg ggccgcatcg
      661 atcgcgtgct gcggctgccg gtaagcaagc cgacgtcgtg cgcgttcggc ggggccgatt
      721 accgcacgct gttcgtgacg acggcgtcga gaggcttgag cgcgtcgcgc ctggcggcgg
      781 agcccgcggc gggacacgtg ctcgcactgg acgtcggtgc tgccggcgtc gcgcccgacg
      841 cgttcattcc ggcatcggcc gcgacgcgca gcgcatcgcg gggtttcgag catggctagc
      901 gactcgcaac gattcaccgg ctatcgccga cgcgacggca gcgtcggcgt gcgcaatcac
      961 gtgctcgtgc tgtcgccgac cggcctcacg tcggccgccg cgcagcgcat tgccgatctg
     1021 gtgctcggga ccgtggcggt gacttccggc ttcgggcgcg ggcaagtggg cgacgatgcg
     1081 caactgcact tcgacacgct ggccgggctt gcacgcaatc ccaatgtcgc ggccgtgctc
     1141 gtcgtatcgg ccgccgacgc gatcaccgag tcctacgtcg acgcgctgcg cgaatcgggc
     1201 aagcccgtcg acggattgtc gctgccgggc gtgcaggagg atgcgctcac gctcgtcgat
     1261 cgcggcgtgc gcatcgccac gcgactggtg cgcgaggcgt cgcaggcgcg tcgcgaatcg
     1321 tcgacgctca ccgatctttt tctcgccgtc gagtgcgggc attccgacgc gacgtccggg
     1381 ctcgtctgca atccgctgac cgggcgcctc gccgagcgca tcgtcggcgc cggcggacgc
     1441 gccgtgttca gcgaaacggt cgagtggacg gggaccgagc atcttctcgc gcgtcgagcg
     1501 gccacgcccg acgtcgcgca gcggatcatc gccaccgtga tggcgcgcga gcatgcggtg
     1561 cgcgaatcgg gcggcgacat ccgcgcgcag aatccggggc cgcaaaaccg gaccggcggc
     1621 ctcacgacaa tcgaggagaa ggcgctcggc gcgatcgcga agggcggcga gcagccgata
     1681 cagggcgtgc tgcgcgcagc ggagcggcct tcgcaatcgg gcctctacct gatggacacg
     1741 ccgtactttt cgcccgagtc gatgacggca atggtggccg cgggcgcgca gatggtggtg
     1801 ttcaccaccg gcgccggcaa cagctactgc agcgcgctcg caccgacgtt caagatgagc
     1861 gcgaatcccg acgcctgcgc acgcctgcgc gagcagatcg actttgccgc aaccggtgtt
     1921 ctcgacggca gcatgacatt cgacgacgcg gccgagcgcg cgctcgacgc gctgctcggc
     1981 gtcgcgtccg gcacgctcac gtacggggaa atcgtcggcg agggcgcgga ggtggtgagc
     2041 cggctggcac catcgattta gcgccgatcg tcctggggca gcggcgccgt cgagtcgttg
     2101 cacgccgtac ggctgcggtc gaatgaaatt caggcggccg cgcgcgtcat gatccgccaa
     2161 tcgtcgagat agcgcgcaag tgccgtcgcg tctcccgtcg catcggcgaa cgcgacgtag
     2221 ccgtccggcc gcacgagata aacggcgtcg cgagcaaggc cggcgcgctg catcgacgcc
     2281 tgccaggcaa acgcatgcag cgctacgctc ctatgctcgc acgcctgctc gacgccgccc
     2341 ggcgcctgcc cgtacacgtg cacctgccag tcgagcgatg aaagtgctgc gtagttgtcc
     2401 gcgacgcgtt gcgttccatc ggcgtggccg acccatggca ggcgatcgcc cgcgtgcacc
     2461 gatccggcgc tgccgcggct cagggcgctt tcgcgatagc ggatcgccgt ttgcgacagc
     2521 gtgcggaaca tgaagcggcg caggacgcgc aggcgcagca gttgcggcag cacgcggggc
     2581 agcacgcgaa tgcgcacgaa gcgggcaatc gcgccgtcgc tggtcgcgag ctggaacgcg
     2641 cgatcggtcg tcgccaccag ttgccgcgcg aatgcgatcc gttccggctc gtacgtgtcc
     2701 agcacgcgcg cgtcgatccg cgatgcggcc gcggccgcga gcttccagcc gagattcgcg
     2761 ctgtcgccga tccccgtgtt catgccttgc ccgcccaccg gactgtggat gtgcgcagcg
     2821 tcgccgagca ggaacgcgcg gccgctgcga aagtgcgaag ccacgcggtg gtgcacgtga
     2881 tacgtcgaaa accagttgac ctgcgttacg tcgatgccga gccgctgcag aaccgtgtgg
     2941 ctcacgtcgc gccactcgag cgcagaagga gggccggaga cgacttccgg tgcctttgcc
     3001 gtctgcaccg ctccgcggtg gaaggtcgtt tccgaccctc cttccttgac ggtgccgatg
     3061 agccgcgcac gcgcatcgcc cttcatcgga aacaccgcaa gcagatcggc ctcgtcgagc
     3121 gccacgtgca gctcgtgatt catcaccggc ccgctcgctt gcacgtcggc cacgtagaaa
     3181 acgtgcgcgt aggtgccgcc ggggaagccc gcctgcagct tttcgcgcac ggtcgaatgc
     3241 gcgccgtcgc atccggcaag gaaggccgcc tcgcatcgct cctcgcgacc gtcgccgtgc
     3301 acaagatgcg cgacgatgcg atcaacgcgc tcgtcgaatc cgcgcaattc gcaaggccgc
     3361 tcgacgcgca cgcccgcggc cgaaagccgc tcgatcagca accgctcgtg ctcatcctgg
     3421 ggaaagatca gcatgaacgc aaacgcggtc gcaccttcgc cgagcgcacc gagatcgaca
     3481 tgcgcagcct gcttcccggc cacccacaga ttcacggccg cgaaacggac cgctgcatcg
     3541 acgacggcgt tggcgatgcc gagctggcgg tagaactcga gcgtgcgtgc gtgaacgacg
     3601 agcgcgcgcg atgtcgttcc cggctcggcg gccttgtcga cgatgcggat gcgcacgccg
     3661 ctttgcgcga gccacagcgc gagcacgagg ccggtcggtc ccgcacccgc gatcagaacg
     3721 tcgacgtcag ccatgggctt cccgcgccgg aaccggcgaa ttctaccgcc ggtgaacgat
     3781 accgcgaggc cgatgcccaa cgaatcactc gcgtaagatg gccaatatcg tcactcccgt
     3841 caggcaactc gtgatggccg attcgacgca ctgggcgttt ccccccgaga tgcaaccgaa
     3901 gagcgacgac gtcggcttcg acttgacgta cgcgctgaac gcggtcgtcg ccatgcgcgt
     3961 ggatgtcgtc gaggacgcgt tcaccgcttc ggtgctcggc accgagcgca ccggcaacgg
     4021 cgtcgtcatc agcgacgacg gcatcgtgct gaccatcggc tacctgatca ccgaagccga
     4081 ctcgatctgg ctcaccacga atggcggcaa cgccgtgccg ggacatgcgc tcgcgtacga
     4141 ccaggtcacg gggctcggcc tcgtgatgcc gctcggctcg ctcggcgtgg caccgctgcc
     4201 gcgcgtcgat cttgcctcgg cggatctcga cgacgacgtc tacgtgatcg ggcacggcgg
     4261 acgcgcgcac gcactgcgcg ccaaggtgtt ctcgcgccgc gagttcgcag gctattggga
     4321 atacctgctc gatgaagcgc tcttcaccac gccggcacac cccgagtgga gcggcgccgc
     4381 gctgctcgat ggacgcggtc gcctcgtcgg catcggctcg ctgttcgtgc aggaggccga
     4441 cggcgatcag tcggtgaagg gaaacatgtt cgtgccgagc gagctgatcg acccgatcca
     4501 ggacgacctg ctgcgcaccg ggcgcgccga tcgcgcgccg cggccgtggc tcggcatcta
     4561 caccaccgag acgaaggatg gcatcgtcgt gcagggcctc gccgacgacg gccctgccgc
     4621 gcgcgcgggc gtcgagcttg gtgacctggt ccgcgccgtg ggcgacgagc cggtcgagcg
     4681 gctcgcgggt ttctatcgca gcgtgtggca gcgcggtgcg gccggtgtcg aggttccgct
     4741 gacgctcgct cgtgacgaca agctctggaa cgtcaccgtg cagtcggtcg atcgtagcga
     4801 gcttttgaag aagcctctgc tgcagtaagc gcgccattgt tcgccgtgcc cggcgcacgc
     4861 tcttcgcgcg acgcggccgg tttctgcgca gacgcatcgc cggccgccgg ctcgaccgcg
     4921 ttctggtaat cccatccgcc gcccagcgcc ttcgcgagat cgacgagtgt caggcgcaca
     4981 tcgcgcgcgg cgaggatcga cagcgtctgc gcctgcaaca gctgccgttg cgcatcgagc
     5041 acctcgagat agggcgaata gccggccctg taccgcaggt ccgacaggtc gtatgcctgc
     5101 cgcaggttgc cgcttctcgt catttgtgcg gcgagtgctg cacgcgtcga ttcgtttgcc
     5161 gccagcgcat cgtgcacatc cttgaacgcc gcgagcaccg tctgccgata attgacgacg
     5221 agctcctgcc gccgcgcctg ctgtgccgcg acgtttgcct cgatcgcctt caggttgagg
     5281 agcggctgca cgagcccggc acccagcgac cagatcaacg ccggaccgct tgcgagatgg
     5341 cgcaaggcgc ccgattccgt tccaagctcg cccgtcagcg tgaccgatgg aaaataatcg
     5401 gcgcgcgcgc gatcgatgcg caggctcgcc gccacgagct ccttctcgac ctcgcgcaca
     5461 tcgggacggc gctcgagcac gtcggacggc agaccggcgg gaaccgttgg cacggcaaga
     5521 agcttcgcga gcgtggtttc gcgggcgacc tgcggggcga acacgtcgcg cggcgagcgc
     5581 ccggtgagga cggcgagccc ggactcgaac tggctgaccg agcgctgcgc cgtcgcgatg
     5641 tcggcgacga ccgcgtcccg ctccgccttc gcctgcgcga ggtcgtattc gccgatcacg
     5701 ccggcctgcg cgcggtcggt ctgcaatgcc acggtctgct cgcgcagcgc aagcgtgtcc
     5761 ttgagaagct gcagttgcgc gtcggcagcc atcagcccga agtacgtctg cgccacggtt
     5821 gccgcgacga cggtgcgcac gctttcgcgc gcgtactcgg tggcgagcag gtcgttttgc
     5881 gccgcgcggg tcgccgtgcg gtacttgccc cacaggtcga tctcgtacga tgcctgcagg
     5941 ccgagccgga agtcgttgcc ggtagcgccg aagccgggcg gcagcgggtt cgtgccgact
     6001 tccgtcacgc ggctgcgccc ggcgttcgca ccgaggttga ggctcggata cagcgcagcc
     6061 gacgccagtg tcacttgcgc gcgggcggtc tccacgcgcg cgatggcggc ctgcagatcg
     6121 aggttgttcg ccagcgcttc gtcgaccagc gcggtgagcg ccggctcgtt gaactcggtc
     6181 caccagtgcg aaagctcgag atggtcgatc gtcggtggcg gaagctcgac tgccggcggc
     6241 gccgtaggcg tcgtcgtgca agctgccagc gcgaaggaca ccgcggcgac agccaatgca
     6301 aagcgcagtg accggtgcgc gcctggaaaa tggctcatcc taatctccgt gcgccggcac
     6361 cggcggatgg cccggcgtat gcggcacgtg gtgcgttgcc gcgcgcgcat gcttgacctc
     6421 gcccagcaac tcgtcgtggc tgcgccgctc gatcagccgc cgctcggcga tcacgcgata
     6481 gaacagcggc acgaagaaca cggcgaggaa ggtcgccgcg agcatgccgc ccatgacgcc
     6541 cgtgccggtc gaatgccgtg cgcccgcgcc ggcgccggtc gacagcgcaa gcggcgtcac
     6601 gccgaggatg aaggcgagcg acgtcatcag gatcggccgg aatcgcaggc gtgcggcttc
     6661 gatcgcggcc gccgacggcg tcatgccttc atgcgtcttc aagaccgcga actcgacgat
     6721 caggatcgcg ttcttggcgg cgaggccgag cagcgtcacg agtccgatct ggaagtaaac
     6781 gtcgttcgtg agcccgcgca accagatcgc ggcgagcgcg ccgaacgtcc cgaagggcag
     6841 cgccagcagc accgaaagcg gcagcgacag ccgctcgtat tgtgcggcga ggatcaggaa
     6901 caccatgatg acggcaaggc cgagcgagaa cgtcgcagca cccgacgatt tcttctcctg
     6961 gtacgacgtg cctccccagt cgtagctgaa gtcggacggc agcacctcgc cggcgatctt
     7021 ctcgatctcg gcgatcgcct gtcccgagct gtagccgggc gcggcctggc cgatgatctt
     7081 caccgcaggc aggttgttga agcgatcgag cgcgtcgggc cccgacgtgt agcggacggt
     7141 gaccagcgac gacagcggca ccatctcgcc cttcgccgag cgcacgtaga cggcgccgat
     7201 gtcgtccggg cggttccgat acgagggctc cgccgacatc agcacctgcc atgcgcggcc
     7261 gtacttgttg aaatcattga tgtagaagtt gcccagcgtc gcggatagcg cggcgaagat
     7321 gtcggagatg ggaatgccga gcgtcttcgc cttctcgcgg tcgacgtcga cgttgagctg
     7381 cggcaccgtt gcgcgccaca gcgtttgcgc gccggccagc accgggtccg aattcgcctt
     7441 ggcgaggaat gcattcttca cctcgtcgag ccgcttggcg ccgccttcgc cgtgattctg
     7501 gatgtagaac tcgaagccgc ccgcggtccc gaggccgaag atcggcggcg ggttgaacgc
     7561 gagcacgagc gcctccttga tgcccgccgt tttgccgaac agctcgccga cgagctgcgg
     7621 tgcggtgacc ttgcgctggt cccacggttt ttgcgtgacg aagatcgtcg ccgcgttgtt
     7681 gcgaaagccg ccgccgatga aatcgaagcc ggtgaacgcg acgatgtcct ggttggcagg
     7741 attcgagcgg atcgcggccg acacctccga cacgaccttg tcggtgcgct cgagcgtcgc
     7801 gccgtcgggc aggatgaccg cgctgatgta gaagccctgg tcctcgtcgg gcacgaggct
     7861 gcccggcgtc gtcctccaga gccaggcggt gacggcaacc atcgaaagga acagcaccat
     7921 gctcaggatg ccgtggcgca gaagaaagat gacgccgtcg ttgtagcgcc ccgtgacgcg
     7981 ggcgaaccag tcgttgaacc agcggaagaa ccggttcggc ctctggtgcg gcttcaggat
     8041 cagcgccgac agggcaggcg acaacgtcag cgcgacgaag ccggaaatca cgaccgacat
     8101 cgcgatcgtc acggcaaact ggcggtagag ctcgccggtc agcccgccga ggaacgcgat
     8161 cggcacgaac accgcggtca gcgtcagcac gatcgcgatg atcggaccgg tcacttcgcg
     8221 catggccttc aatgctgcct cgcgcgccgg cagatggtcc tcgcgcatgt gccgctcgac
     8281 gttctccagc acgacgatcg cgtcgtcgac gacgatgccg atcgcgagca ccatcgcgaa
     8341 cagcgtcagc gtgttgatcg agtagccgag catataaatg cccgcgaagg cgccgatcag
     8401 cgagaccggc acggcagcga acggaatgat cgctgcgcgc cagctctgca ggaacaggta
     8461 gacgacgagg aagacgagca gcatcgcctc gccgagcgtc ttcaggacct cgcggatcga
     8521 cacctcgacg aagcgcgtcg tgtcgtaggg gacggcgtac gagaggcctt ccgggaagcg
     8581 cgtcgcgagc gtcgcgacct cggcgcgcac gctcttcgcg acgtcgagcg cattggcgcc
     8641 cggcgacagg aaaatgccga cgagcgtcgc gtccttgccg ttgaagcgcc cgatgaactc
     8701 gtagtccttc gacccgagct cgacgcgcgc gacatccccg aggcgcaccg tcgacccgtc
     8761 cggattcgcg cggacgatga tctgcgagaa ttcgttggca tcggtgaggc ggcccttggt
     8821 cgtgatcgtg tagacgagcg actggttcga aggcgacggc gcctgtccga ccttgcccgc
     8881 ggcgaactgc gcgttctgtt cgttgactgc ggcgatcacg tcgcccggcg tcaggcgaag
     8941 ctgcgcgagc cggtcgggct tcaaccatat gcgcatcgcg taatcgtgcg caccgaagat
     9001 ctgcacgttc gtcgtgccgg gcagccgctt cagcgcgtcg agcacgttga ggttgacgta
     9061 gttcgacgtg aagaggtcgt tgtagcggcc gtccggtgaa tagaacgcga gaacttgcag
     9121 gaacgccgag ctgccgcgct cgacggtgac gccctgccgc cgcacttcct cgggcaggcg
     9181 cggcagcacc tggttgacgc ggttgttgac gttgaccgcc gcgatgtcca cgttcgttcc
     9241 gatgtcgaac gtcacctgga tctgcacgat gccgttcgac gccgacgagg agctcatgta
     9301 gatcatcccc ggcacgccgt tgatcgagtt ctcgatcggc gatgccaccg tctgctcgag
     9361 ggtttgcgcc gacgcgccgg gatacacggc ctgcacggtg acgaccggcg gcgcgatctc
     9421 gggg
//
LOCUS       DASSVX010000256         5921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_828236, whole genome shotgun sequence.
ACCESSION   DASSVX010000256 DASSVX010000000
VERSION     DASSVX010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828236"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VFO53_11000"
     CDS             <1..724
                     /locus_tag="VFO53_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HET8876846.1"
                     /translation="RGEIAFTLGDSLSDAWKGNEEAGFKTPLKKLRGVAAIYPNYIQI
                     VARADAGIKTLADLKGKKISVGAPKSGTELNARAVLHAAGIEYKDFAKVEYLPFGESV
                     ELMKNRQIDVTLQSAGLGVSSLRDLAASVPIVVVPIPEDVIKKINDAAYIHAVVPANT
                     YNGQASDVSTAAVQNFLVTHEGVSDEIVYGMTKALWTNLDQLVAAHSAAKAIDIKKAT
                     QGMPVPLHPGAEKYYREVGILK"
     gene            773..2920
                     /locus_tag="VFO53_11005"
     CDS             773..2920
                     /locus_tag="VFO53_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HET8876847.1"
                     /translation="MKKVVSDPAAAANAATRLAQPDEPAFDETGVKRVLAGWDARAVA
                     AIALCFSTYQLVIAAYAPLSSLPTRSIHVGFLLALAFLIHPVARWVNRRRIAWYDALI
                     AAGAFALSLYHLAFEADLIQRAGDPSLADLVVGAVFIVLLFEAARRVLGVALPIICGV
                     FLAYGLFGQYLPGVLAHRGYGIDQIIGQLFLGTEGIFGIPTLVSATYIFLFILFGSFL
                     EHAGMINLFNSIALGFVGHARGGPAKVAVISSGMMGTISGSGIANVLTVGQFTIPLMK
                     RFGYSSIFAGAVEATSSMGGQIMPPVMGAVAFIMAETLNVPYVTIVKAAVVPAILYYA
                     TAFWMVHLEAGRAHLLGIPKSECPNPWVAIRQKWYLMLPLVVLVYMLFDGFTPMFAGL
                     VGLALTATLILGAAVAARVGPTAFRYAFWIALGMGLGAYMRFGFDRHGITPIIAGIGV
                     LVAITAAIRGRETLVTMRQSLVDGARQAVTVGLACAIVGVIIGVLTLTGAASNFAEFV
                     LHVGSRSLFLSLLLTMVVCLILGMGIPTIPNYIITSAIAAPALLKLGVPLIVSHMFVF
                     YFGIMADLTPPVALAAFAAASIAKGPPMRIGWKATHIAIAGFVIPYMAVYDPALMLQA
                     SDAGGSVSVIAAVYVIAKAILAIGLWGGAAIGYFLRGPLAWPERVIAFAAASLLVAAV
                     PWTDQAGFIASAVFILWHLWRTRGVPEPVKAPT"
     gene            2925..3344
                     /locus_tag="VFO53_11010"
     CDS             2925..3344
                     /locus_tag="VFO53_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HET8876848.1"
                     /translation="MHTACLFIAGALRAVIPADHFTVAWTHSVEKTRWEERYRVEASG
                     LRLTEARIQGSGAGMDPPPDAILRDGWWSWKPSVGRLPKLRLTLSPFTRDYDLCWGGR
                     CHKLQRLVAAVQAPDAARPGPGAIAVVEVRSCSESTP"
     gene            3341..3715
                     /locus_tag="VFO53_11015"
     CDS             3341..3715
                     /locus_tag="VFO53_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HET8876849.1"
                     /translation="MNEWFRKVARALADAFGSPWAFVVAAILAVLWGVTGPLFRFSDA
                     WQLIANTVTNVGTFLMVFVIQNSQNRDTKETQLKLDELLRAISTARTSLVNLDTLSDD
                     EIEHLHRQFEALGRRAAADKPG"
     gene            complement(3752..4321)
                     /locus_tag="VFO53_11020"
     CDS             complement(3752..4321)
                     /locus_tag="VFO53_11020"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET8876850.1"
                     /translation="MKFCSVCGSAQIEHRVPDGDTLPRDVCTNCGTIHYQNPKVVVGC
                     LPEWEDRVLLCRRAIEPRYGLWTLPAGFLENGETLADGALRETLEEAHARVAIGELCA
                     VISLPQINQVYMMFRARLLDLAFAAGAESLEVRLFDERDIPWESIAFRTIARTLRNYF
                     LDRRLGGFALHVSAIERRSPLTPDLLAAG"
     gene            complement(4350..4895)
                     /gene="orn"
                     /locus_tag="VFO53_11025"
     CDS             complement(4350..4895)
                     /gene="orn"
                     /locus_tag="VFO53_11025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HET8876851.1"
                     /translation="MPQDPNRLIWIDLEMSGLRPDSDRILEVALVITDHALETVAQAP
                     VWVVHQQDAVLDGMDAWNRSTHARSGLIDKVRASSATEQEVEAAALAFLREHVPAKAS
                     PMCGNSICQDRRFLARFMPALEDWFHYRNLDVSTLKELARRWKPELGKGMPKEGRHEA
                     LADVLESIAELRYYRDNFLRL"
     gene            4967..>5921
                     /locus_tag="VFO53_11030"
     CDS             4967..>5921
                     /locus_tag="VFO53_11030"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET8876852.1"
                     /translation="MTADAFALVFVIAVAVTLALRLWLAARQIAHVRAHRVATPAAFA
                     ARIDDTAHRKAADYTIARQRLGMLESIVDATLLLVLTQGGALAWILAATQEPALPPLV
                     RDVLLLCGVAFAGGIVSLPFSWWRTFRIEERFGFNRTTLATWLADLAKGIAVGAVLGL
                     PLAFVVLWLMRGAGAYWWLFVWIAWMAFQLLVLVLYPTVIAPLFNRFSPLPAGAQRDR
                     IERLLQRCGFTASGLFVMDGSRRSGHGNAYFTGFGRAKRVVFFDTLLARLAPDEIEAV
                     LAHELGHFRLRHVVKRVAWSSVASLALLGLLAWLAQSPWFYA"
BASE COUNT          922 a         1990 c         1911 g         1098 t
ORIGIN      
        1 ccgcggcgag atcgcgttca cgctcggcga ttcgctgtcc gatgcgtgga agggcaacga
       61 ggaagccggc ttcaagaccc cgttgaaaaa gttgcgcggt gtcgcggcga tctatccgaa
      121 ctacatccag atcgtcgcgc gcgccgacgc gggcatcaag acgcttgccg atctcaaggg
      181 caagaagatc tcggtgggcg cgccgaagtc gggcaccgag ctcaacgcac gcgcggtgct
      241 gcacgcggcg ggcatcgagt acaaggactt cgcgaaggtc gaatacctgc cgttcggcga
      301 atccgtcgag ctcatgaaga accgccagat cgacgtgacg ctgcaatcgg cgggactcgg
      361 cgtatcgtcg ctgcgcgacc tcgcggcgtc ggtgccgatc gtcgtggtac cgattcccga
      421 agacgtgatc aagaagatca acgacgctgc gtacatccac gcggtcgtgc cggcgaacac
      481 gtacaacgga caggcgagcg acgtcagcac ggccgccgtg cagaatttcc tcgtgacgca
      541 cgaaggcgtc agcgacgaaa tcgtctacgg catgacgaag gcgctgtgga cgaacctcga
      601 ccagctcgtt gccgcacact cggccgccaa ggcgatcgat atcaagaagg cgacgcaggg
      661 gatgccggtg ccgcttcatc cgggcgcgga gaaatactac cgggaagtcg gcatcctcaa
      721 gtaaccgggg cacagagacg actttctgca acgcagggtc acggcacagc caatgaaaaa
      781 ggtcgtgtcc gaccctgctg cggctgccaa cgctgccacc cgccttgcgc aacccgacga
      841 gccggcgttc gacgagacgg gcgtcaagcg tgtgctcgca ggctgggacg cacgcgcggt
      901 cgccgcaatc gcgctctgct tttcgaccta ccagctcgtc attgccgcgt atgcgccgct
      961 gtcgagcctg ccgacacgct cgatccacgt cggcttcctg cttgcactcg cgttcctgat
     1021 ccatccggtc gcgcgctggg tcaaccggcg ccgcatcgcc tggtacgacg cgctgatcgc
     1081 cgccggcgcg ttcgcgctca gcctctatca cctcgccttc gaagccgacc tcatccagcg
     1141 tgcgggtgac ccgagcctcg ccgatctcgt cgtcggcgcg gtgttcatcg tgctgctgtt
     1201 cgaggcggcg cgacgcgtgc tcggggtcgc gctgccgatc atctgcggcg tgttcctggc
     1261 ctacggcctc ttcggccagt acctgccggg cgtgcttgcg caccggggct atggcatcga
     1321 ccagatcatc gggcagctct tcctcggcac cgaaggcatc ttcggcatcc cgacgctcgt
     1381 ctccgcaacg tacatcttcc tgttcatcct gttcggcagc ttcctagagc acgccggcat
     1441 gatcaacctc ttcaacagca tcgcgctcgg cttcgtcggt cacgcgcgcg gcggaccggc
     1501 gaaggtcgcc gtgatctcat cggggatgat gggcacgatc tcgggctcgg gcatcgcgaa
     1561 cgtgctcacc gtcggccagt tcacgattcc gctgatgaag cgcttcgggt attcgtcgat
     1621 tttcgccggc gccgtcgagg cgacgtcgag catgggcgga cagatcatgc cgccggtcat
     1681 gggcgcggtc gcgttcatca tggccgaaac gctcaacgtg ccgtacgtga cgatcgtcaa
     1741 ggcggcggtg gtgcccgcga tcctctacta cgccacggcg ttctggatgg tgcacctcga
     1801 agcgggccgt gcgcatcttc ttggcattcc caagtcggaa tgcccgaatc cgtgggtcgc
     1861 cattcgccag aagtggtacc tgatgctgcc gctggtggtg ctggtctaca tgctgttcga
     1921 cggctttacg ccgatgttcg cgggcctcgt cgggctcgcg ctcaccgcga cgctgatcct
     1981 cggcgcggca gtggcggctc gcgtggggcc gaccgcgttc cgctatgcat tctggatcgc
     2041 gctcggcatg gggctcggtg cgtacatgcg cttcggattc gatcgtcatg gcatcacgcc
     2101 gatcatcgca ggcatcggcg tgctcgtcgc gatcaccgcc gcaatccgcg gccgcgagac
     2161 gctcgtcacg atgcggcaaa gtctcgtcga cggcgcgcgg caggcagtca ccgtcggtct
     2221 cgcctgcgcc atcgtcggcg tgatcatcgg cgtgctcacg ctcaccggcg cagcgtccaa
     2281 cttcgccgaa ttcgtgctgc acgtcggcag ccgcagcctg ttcctgtcgt tgctgctcac
     2341 gatggtcgtg tgcctgatcc tcggcatggg cattccgacg attccgaact acatcatcac
     2401 gagcgccatc gcggcgccgg cgctgctgaa gctcggcgtg ccgctgatcg tgtcgcacat
     2461 gttcgtgttc tatttcggca tcatggccga cctgacgccg ccggtggcgc tcgccgcatt
     2521 cgcagcggca tcgatcgcca agggcccgcc gatgcgcatc ggctggaagg caacgcacat
     2581 cgccatcgcg ggcttcgtca ttccgtacat ggcggtctac gatccggcac tgatgctgca
     2641 ggcgtccgac gccggcggca gcgtgtcggt gatcgccgcg gtctatgtga tcgcgaaagc
     2701 gatcctcgcg ataggtttgt ggggcggtgc cgccatcggt tatttccttc gcgggccgct
     2761 cgcatggccc gagcgggtga tcgcgtttgc cgccgcatcg cttctcgtcg ccgccgtgcc
     2821 gtggaccgac caggccggct tcatcgcgag cgccgtcttc atcctctggc atctgtggcg
     2881 cacgcgcggc gtgccggagc cggtgaaggc gccgacgtag tcgcgtgcac acggcttgcc
     2941 tgttcatcgc gggtgcgctg cgtgctgtca tccccgccga tcacttcacc gtcgcgtgga
     3001 cccattccgt cgaaaagacg cgctgggagg agcgctatcg cgtcgaagcg tccggcttgc
     3061 gcctcaccga agcgcgcatc cagggctcgg gcgcgggcat ggatccgcca ccggacgcga
     3121 ttcttcgcga cggctggtgg tcatggaagc cgtccgtcgg gcgattgccg aaattgcggc
     3181 tgacgctgtc gccgttcacg cgcgactatg atctttgctg gggcggccga tgtcataagc
     3241 tgcaacgcct cgtcgccgcg gtgcaggcgc ccgatgctgc gcggcccggc ccgggcgcga
     3301 tcgccgtcgt cgaagtccga tcctgcagcg aaagcacgcc atgaacgagt ggttccgcaa
     3361 agttgcccgc gcactcgccg acgccttcgg ctcgccgtgg gcgttcgtcg tcgcggcgat
     3421 cctcgccgtg ctgtggggcg tgaccggtcc gctgttccgc ttctcggacg cctggcaact
     3481 cattgcgaac accgtcacca acgtcgggac gttcctgatg gtgttcgtga tccagaacag
     3541 ccagaaccgc gatacgaagg aaacgcagtt gaagctcgac gagctgttgc gcgcgatctc
     3601 cactgcgcgt acgagtctcg tcaatctcga cacgctgtcc gacgacgaaa tcgagcatct
     3661 gcaccggcaa ttcgaggcgc tggggcgtcg cgcggcagcg gacaagccgg gctgatacgg
     3721 cgaccgcgtg ctccgccgcc gccgcgtggc gtcaaccggc ggcgagcaag tcgggcgtga
     3781 gcggcgagcg acgctcgatc gcgctcacat gcagcgcgaa cccgccgagc ctgcgatcga
     3841 ggaaatagtt gcgcaacgtc cgcgcgatcg tgcgaaacgc gatcgactcc catggaatgt
     3901 cgcgctcgtc gaacaggcgc acctcgaggc tctcggcacc cgcggcgaac gcgagatcga
     3961 gaagccgcgc gcgaaacatc atgtacacct ggttgatctg cggcaggctg atcaccgcgc
     4021 agagctcgcc gatcgccacg cgcgcatgcg cctcctcgag cgtctcgcgc aacgcgccgt
     4081 ctgccagcgt ctcgccattc tcgagaaatc ccgccggaag cgtccacaat ccgtagcgcg
     4141 gctcgatcgc gcgccggcag agcaggacgc gatcctccca ttcgggcagg caaccgacga
     4201 cgaccttcgg attctggtaa tgaatcgtcc cgcaattcgt gcacacgtca cgtggcagcg
     4261 tgtcgccgtc aggcacgcga tgctcgatct gcgccgatcc gcagacgctg cagaatttca
     4321 cccgcgcgac cgccacgtct gcacgctggt cacagccgca aaaaattgtc gcggtagtag
     4381 cgcagctccg cgatcgactc gagcacgtcg gcgagcgcct cgtgccgtcc ctccttcggc
     4441 atgcccttgc cgagctccgg cttccagcga cgtgccagct ccttgagcgt ggagacgtcc
     4501 agattgcggt aatggaacca gtcctcgagc gccggcatga agcgcgcgag aaagcgccgg
     4561 tcctggcaga tcgaattgcc gcacatcggt gacgccttcg ccggcacgtg ctcgcgcagg
     4621 aacgcgaggg cggccgcttc gacttcctgc tcggtcgcgc tcgacgcacg caccttgtcg
     4681 atgaggcccg agcgcgcatg cgtcgaccgg ttccacgcgt ccatgccgtc gagcaccgcg
     4741 tcctgctggt ggacgaccca gacaggcgcc tgcgcgacgg tttcgagcgc gtgatcggtg
     4801 atcacgagcg cgacttcgag gatgcggtcg ctgtcgggcc gcagccccga catttcgagg
     4861 tcgatccaga tgagccgatt gggatcctga ggcatgtcgg ggcgtaaaat gacttgcatt
     4921 ccattgtacg tgagcgcggt gcgatgcctg ccgccgcgca attgccgtga cagcagatgc
     4981 attcgcactc gtcttcgtca tcgcggtcgc ggtgacgctt gcgctgcgcc tgtggcttgc
     5041 cgcgcgccag atagcgcacg tgcgtgcgca tcgcgtcgcc acgccagccg cattcgcggc
     5101 acgcatcgac gacaccgcac atcgcaaggc ggccgactac acgatcgcca ggcagcggct
     5161 cggcatgctc gagtcgatcg tcgacgccac acttcttctc gtgctgaccc agggtggcgc
     5221 gctcgcgtgg atcctggcag caacgcagga gccggcactt ccgccgctcg tgcgcgacgt
     5281 gcttctgctg tgcggcgtgg cattcgccgg cggaatcgtt tcgcttccgt tctcgtggtg
     5341 gcgcacgttt cgcatcgagg agcgattcgg attcaaccgg acgaccctcg cgacctggct
     5401 cgccgatctc gcgaaaggga tcgcggtagg tgcggtactc gggctgccgc tcgcgttcgt
     5461 cgtgctgtgg ctgatgcgcg gcgccggcgc ttactggtgg ctgttcgtct ggatcgcgtg
     5521 gatggcgttc cagctgctcg tgctcgtgct gtatccaacc gtgatcgcgc cactgttcaa
     5581 tcgcttttcg ccactgcccg caggtgcgca gcgcgatcgc atcgagcgtc tgctccagcg
     5641 gtgcggcttc acggcgtcgg gattgttcgt catggacgga tcgaggcgtt ccgggcatgg
     5701 caatgcctat ttcaccggct tcggccgcgc aaagcgcgtc gtcttcttcg acacgctgct
     5761 cgcgcgcctg gcacccgacg aaatcgaggc cgtgctcgcg cacgagctcg ggcatttccg
     5821 gttgcgccac gtcgtcaagc gcgtggcctg gtcgtcggtg gcttcactcg cgctccttgg
     5881 cctccttgca tggcttgctc agtccccatg gttctacgcg g
//
LOCUS       DASSVX010000257        11544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_261553, whole genome shotgun sequence.
ACCESSION   DASSVX010000257 DASSVX010000000
VERSION     DASSVX010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 11544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11544
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261553"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFO53_11035"
     CDS             complement(<1..280)
                     /locus_tag="VFO53_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018828459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET8876853.1"
                     /translation="MSGRTFLDILAGAEAIAARAAQTLVAMHDRPHEVARKQLRDVVT
                     EADLASERLVIDGLRALTPGAAILSEEAGASGPDGGTRWIVDPLDGTVN"
     gene            complement(277..1548)
                     /locus_tag="VFO53_11040"
     CDS             complement(277..1548)
                     /locus_tag="VFO53_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876854.1"
                     /translation="MNKGFLCAIALACVSMVGSARAEEVTLDVYYAFPSFAKFHEPIA
                     KEFMQSHPDIRIVFRAPAPTYDDGHQTMMRSAVTNRLPDVYYSGYDLLEELARALAKR
                     NQIVDVGPLLAAEGDAFRAKNYAGSILGLGKVDGKLYGLPFNASTPIVYYNVELVKKA
                     GGDPDRMPDTWDATMALAAKIHALGADVAGLAYDVHDYPGNWLYHSMIMQAGGVVVDG
                     DRVPLGGPDIGVKVMQTFRRFVTEDGMPLIALDQSRQQFFAGKVGLFFDTPGRLKQIT
                     DLVGDKFTMRTATFPVDDKARGGLPTGGNAAIITTRDPKKQKAAWEYLKFVTGPEAQK
                     IVVETTGYMPTNLRANDAAFLGAFYDKNPNYRTASLQTDRAARWVGYPAGNSVRIWRA
                     QRDIINAVMRGDLDPKAGYEKIVKDTAGMMQ"
     gene            complement(1577..2470)
                     /locus_tag="VFO53_11045"
     CDS             complement(1577..2470)
                     /locus_tag="VFO53_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HET8876855.1"
                     /translation="MTLARREAAPLTSPLAIPPNMIIAQITDLHARPPGVRAYAGIDT
                     NAMMRSAVDAIAALDPLPDCVIATGDLTDCGLPAEYQQVGDAFAQLPIPAFLIPGNHD
                     RRDVMRACLAGRHRYLTQDADFLHYVIDDFPVRLIALDTVVGGDHGGEICPAREAWLE
                     RTLAQGGGKPTLIFMHHPPFRTGLPAMDSMMCRSAPTFARLIARHPEIERVVAGHCHR
                     PIVVRWAGTVGFVAPSTAHQVVLDFESADDNRFIHEPPGLALHVYRPGIGIVSHVAVI
                     GDYGPIHSFRLPAEYPGGHPA"
     gene            complement(2467..3747)
                     /locus_tag="VFO53_11050"
     CDS             complement(2467..3747)
                     /locus_tag="VFO53_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004439693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876856.1"
                     /translation="MKRMASVLGSILLAAIVMATGARAEEVTLDVLYAFPAFAKFHEP
                     IAQEFMKRHPDIKIKYRAPAPTYDDGHQTMMREAVTNQLPDVYYSGFHLLTELTHTLE
                     QRKQIVDLGPLLAAEGAEFRKANYSDRILTLGKVDGKLYGLPFNASTPIMMYNAELVK
                     KAGGDPDHMPDTWDGIMALAKKIKASGGADVAGIAYNVNDWPDVWLYHAMILQAGGTV
                     VDGKNVPLGGPDLGVKVLDRFRRFVSEGGMPLINWDQSRQQFIAGRIGIFFDTPARLR
                     QVTDLIGDKFTLRTTTFPIDNKAKGGLPTGGNAALITTHDAKKQKAAWEFIKFETGPE
                     AQKIVVETTGYLPTNLRAPGPEFLGPFYEKNPNFKTVSLQMERSVPWQGYPGGNSVRI
                     WRAQREIITSVMRGDIAPKAGYDRLMKETATLMQ"
     gene            complement(3783..4598)
                     /locus_tag="VFO53_11055"
     CDS             complement(3783..4598)
                     /locus_tag="VFO53_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET8876857.1"
                     /translation="MLTLLIVHGVLIAGAMFILLPFVWMVVTSIKSPPEIFAVDFSLW
                     PKHFNAVENYGNALGKVPLLRFALNGVIVCGVILLVQVLIAVPCAYALAKLRFPGRAT
                     MFALVLLGLCIPIQVPALPLYMGLAKLGLLNTYFAIMMPFFLSVFAIFLFRQFFRSYP
                     DEIIHAARLDGMSEFEIVWRIVVPSAWPAIAAFSVFSLVAHWNDLYWPLIVISDTHLA
                     TPPLGMMFFADAESGSNYGALTAAATVLTAPLVIAFLIARRRFIQGITMTGIK"
     gene            complement(4676..5602)
                     /locus_tag="VFO53_11060"
     CDS             complement(4676..5602)
                     /locus_tag="VFO53_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011910469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8876858.1"
                     /translation="MTAAAAMASPAALPPRNRQQLIERLAGCGFVFPAFALLLLIDII
                     PLAVLLYLSFTDYRLGALDTHFLGLDNFSKALADPVFRRSITNTFVYVAIVLPLAVGL
                     GLLVAVLLHGRKRTRSFYEVVYFLPVTSTLIAMATVWQFLLHPSLGPINGMLRALGFH
                     EMAFLSEPAYVLPTLAAIGIWHLVGFNLVLFLAGLSAIPRDLYEAAEIDGCRSGIDRF
                     LTITWPLLGPTTMFVIVTTSITAFKVFDTVAVMTRGGPMGSSEVLLYAIYLEGYQYFH
                     MGYAAALTLVFLVFVLLFSILQTFVLDRRVHY"
     gene            complement(5619..6821)
                     /locus_tag="VFO53_11065"
     CDS             complement(5619..6821)
                     /locus_tag="VFO53_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009806879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876859.1"
                     /translation="MANVELRAIGKSFGDVSVLSGIDLDIADGEFLTLVGASGCGKST
                     LLRIIAGLEVQSSGSVLIDGAPVDHLRPHERRVAMVFQSYALYPHMRVFGNIALPLVM
                     SRLNLFDRLPLLRALSLRRRRVMPEIAREVHAVAAQLQIDALLERRPSQLSGGQRQRV
                     ALGRAMVRQPAAFLMDEPLSNLDAKLRVHMRTELADLHARLGATFIYVTHDQVEAMTM
                     SNRVAMMDNGRILQLGRPGELYARPASVKVAQFIGTPAINLLPARVSAQHSVELFGGE
                     LPIRVPRPAGAALTLGIRPEAITPAPLGAPAAGRHVLAGRLHRSENLGAEHILHVDLA
                     QPARGVVICKVAADPESLVDGARDLALQFSPAACHVFDDEGHRVADAEVPNAASGAGA
                     LPMSAVSP"
     gene            complement(6852..7676)
                     /locus_tag="VFO53_11070"
     CDS             complement(6852..7676)
                     /locus_tag="VFO53_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8876860.1"
                     /translation="MARVLQISDTHLSPAKRQFAANWAPLRDWALRQHADLIVHTGDL
                     TVDGADSEEDMRDGAALMRALPARVLAVPGNHDVGEAGNRFQPVNAERLDRWRRHFGA
                     DRWSHDLPAWRLIGIDSMLFGDGSDEEARQFECLVQTMATAGERRIAWFTHRPLFIDA
                     PDEADTGYWSVKPAPRARLLELVERHDVALIATGHLHKWRDTKVGRCRYVWCASSGFL
                     VGPDNQPDMPGEKRLGAVIYEFGDADVSVTPADIPGLTTLWIDDVLHEVYPPRHAA"
     gene            complement(7774..9315)
                     /locus_tag="VFO53_11075"
     CDS             complement(7774..9315)
                     /locus_tag="VFO53_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET8876861.1"
                     /translation="MRSAERRRRNVLLIVVDQWRGDLLPKLGTPHLRTPNLDRLCAAG
                     VTFRNHFTTTSPCGPARASLLTGLYQMNHRAVQNTIPLDARFTNLGKELRNGGYDPAL
                     IGYTTTTPDPRTTSPHDPRFRILGDVMDGFRVVGGFEPSKEPYYAWLASRGFALPRVR
                     EEIWLPKTGDPGASAASSMLSAEDSDTSYFTERALAYLRGAHDKPWFLHLGYWRPHPP
                     FIAPAPYHAHYDPADMPPPIRAATPDDEARQHPLLAYYVRHLAQSNFFVNARGLASDM
                     SEAQVRRMRAAYYGLMTEIDDHLGRVFAFLDETNQWKDTLIVFTCDHGEQLGDHHLLG
                     KNGYFDQTFRIPMVVSDPDPRANSTRGTIVDSFTESVDTMPTVLEWLGLSIPRQCDGR
                     SLLSFCHGAAPRDWRTEVHYEFDFRDVYYSQPESSLGLPMDRCSLAVVRDADYKYVHF
                     AALPALFFDLREDPNEFIDRSGDPGYAARIRDYAQKMLSWRLAHADKALTGYRATPDG
                     LECRA"
     gene            complement(9324..10277)
                     /locus_tag="VFO53_11080"
     CDS             complement(9324..10277)
                     /locus_tag="VFO53_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET8876862.1"
                     /translation="MIDDKQTEVRAAWLYFMEGLTQAEIARRLGTTRLRINRILVDAR
                     RNGLVGITLNADLATCVELEQQMIRDFKLKAAVIVPTPDDDEQIPVIVGRAAAGFVSH
                     YLENNGTRGLGIGWGRTLREMIRHMRAGRYPDLGVNSMMGGLTHGLEFNTFDLASELA
                     RRLHSQCRYLVAPMYAGSPRSRDTIMAQDVFRDTLRCIETSDIAVLSVGDVTRRSMLV
                     RYALPDDVSVTELRAAGAVCDIVAQFIDERGRAIDHPINRRAIALPLRDLSRIPTVVF
                     AAGGRHKVRAIASVLRAGLGSVFVCDEATARAAVKLAQDAA"
     gene            complement(10357..10812)
                     /gene="rpiB"
                     /locus_tag="VFO53_11085"
     CDS             complement(10357..10812)
                     /gene="rpiB"
                     /locus_tag="VFO53_11085"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019952416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET8876863.1"
                     /translation="MRIAIGCDEAAYELKEILRKHLAATGVELQDYGTFDAAPVLYPD
                     IAFAVAEAVASGRHDRGILLCGTGIGMAISANKVPGIRAAQAHDTDSAARARMSNDAQ
                     IVTIGARVVGSELAKSIVDAFLASEFQGGRSKDKVARIVAYETRKPGSP"
     gene            10956..>11544
                     /locus_tag="VFO53_11090"
     CDS             10956..>11544
                     /locus_tag="VFO53_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008031193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET8876864.1"
                     /translation="MRYVIGIDGGTESLRAHVFDLHGNSAGSAKGDYATAFPAPGRAE
                     QHPRDWWRALGEAVRGAVTAAGIIARDVAALALDTTSATVVVTDAAGDPMRAAILWMD
                     VRADAEAAAVLHTHDDALRVNGAGTGPVSPEWMIPKALWVKRHERALYDRCTTICEYQ
                     DYMLRRLTGRNVGSLSNVAIRWHYRTREGGWPRTLL"
BASE COUNT         2006 a         3766 c         3893 g         1879 t
ORIGIN      
        1 agttgaccgt gccgtcgagc gggtcgacga tccagcgcgt gccgccgtcc gggccgctcg
       61 cgcccgcctc ttcggaaaga atcgccgcgc cgggcgtcaa cgcgcgcaag ccgtcgatca
      121 cgagccgctc cgacgcgaga tccgcctcgg tgacgacgtc gcgcaattgc ttgcgcgcga
      181 cctcgtgcgg ccggtcgtgc atcgcgacca gcgtctgcgc ggcgcgcgcc gcgatcgcct
      241 cggcgccggc gaggatgtca agaaaggttc ttccgctcat tgcatcatcc ccgcggtgtc
      301 cttgacgatc ttctcgtagc ccgccttcgg gtcgaggtcc ccgcgcatca ccgcattgat
      361 gatgtcccgc tgcgcgcgcc agatgcgcac cgagttgccg gccggatagc caacccaccg
      421 cgccgcgcgg tcggtctgca gcgaggcggt gcgatagttg gggttcttgt cgtagaacgc
      481 gccgaggaac gccgcgtcgt tggcgcgcag attggttggc atgtagccgg tcgtttcgac
      541 gacgatcttc tgcgcttcgg gaccggtgac gaacttgagg tactcccacg ccgccttctg
      601 ctttttcgga tcgcgcgtcg tgatgatcgc cgcattgccg ccggtcggca ggccgccccg
      661 cgccttgtcg tcgaccggga acgtggccgt gcgcatcgtg aacttgtcgc cgaccaggtc
      721 ggtgatctgc ttcaaccggc ccggcgtgtc gaagaacagg ccgaccttgc cggcgaagaa
      781 ctgctggcgc gactgatcga gcgcgatcag cggcatgcca tcctcggtga cgaagcggcg
      841 aaacgtctgc atgaccttga cgccgatgtc ggggccgccg agtggcacgc gatcaccgtc
      901 gacgacgacg ccacccgcct gcatgatcat cgagtgatag agccagttcc ccggatagtc
      961 gtgcacgtcg taggcgagac ccgcgacgtc ggcgcccagc gcgtgaatct tcgcggcgag
     1021 cgccatggtg gcgtcccacg tgtcgggcat gcggtcgggg tcgccaccgg ccttcttcac
     1081 gagctcgacg ttgtaataga cgatcggcgt cgacgcgttg aacgggaggc cgtacagctt
     1141 gccgtcgacc ttgccgagac cgaggatgct gccggcgtaa ttcttcgcac ggaatgcgtc
     1201 gccttcggcg gccagcagcg ggccgacgtc gacgatctgg ttgcgcttcg cgagcgcgcg
     1261 ggccagctcc tcgagcaggt cgtagcccga gtaatagacg tcgggaagcc gattggtgac
     1321 cgccgagcgc atcatcgtct gatggccatc gtcgtaggtc ggcgccggcg cccggaacac
     1381 gatccggatg tccggatggc tctgcatgaa ttccttcgcg atcggctcgt gaaacttggc
     1441 gaaggacggg aacgcatagt agacgtcgag ggtgacctcc tcggccctcg cgcttcccac
     1501 catcgacacg caggccagcg cgatcgcgca cagaaagccc ttattcatcg atcctcccct
     1561 cgtgaagcga tcgcgcctac gccggatgcc cgcccggata ctcggcggga agcctgaagc
     1621 tgtggatcgg cccgtagtcg ccgatgaccg cgacgtggct gacgatgccg atgccgggac
     1681 ggtacacgtg cagcgcgagc cccggcggct cgtggatgaa acgattgtcg tccgcacttt
     1741 cgaaatcgag cacgacctga tgcgcggtgc tcggcgcgac gaagcccacc gtgccggccc
     1801 agcgcacgac gatcgggcga tggcagtggc cggcgacgac gcgctcgatc tcggggtggc
     1861 gcgcgatcag cctggcgaac gtcggcgcgc tgcggcacat catcgagtcc atcgccggca
     1921 aaccggtgcg aaacggcgga tgatgcatga agatcagcgt cggtttcccg ccgccttgcg
     1981 ccagtgtccg ctcgagccac gcctcgcgcg cggggcagat ctcgccgcca tggtcgccgc
     2041 ccacgacggt gtcgagcgcg atcaaccgca cggggaaatc atcgatgacg tagtgcagga
     2101 agtcggcatc ctgagtcaga taccgatggc gcccggcaag gcacgcgcgc atgacatcgc
     2161 gccgatcgtg attgccggga atcaggaacg cgggaatcgg caattgcgcg aacgcatcgc
     2221 ccacctgctg atattccgcc ggcaagccgc aatcggtgag gtcgcccgtg gcgatgacgc
     2281 agtcgggcag cggatcgagc gcggcgatcg cgtcgaccgc gctgcgcatc atcgcgttgg
     2341 tgtcgattcc ggcgtaggcg cgcacgcccg gcggacgggc atgaagatcg gtgatttgcg
     2401 caatgatcat gttcggaggg attgcgagag gcgacgtgag cggcgccgcc tcgcggcgcg
     2461 caagcgtcat tgcatcagcg tcgccgtttc cttcatcagc cggtcgtagc cggccttggg
     2521 cgcgatgtcg ccgcgcatga cactggtgat gatctcgcgc tgggcgcgcc agatccgcac
     2581 ggagttgccg cccggataac cttgccacgg caccgatcgt tccatctgca gcgaaaccgt
     2641 cttgaagttc ggattcttct cgtagaaggg accgagaaac tccggtccgg gcgcgcgcag
     2701 gttcgtcggc agataacccg tcgtctcgac gacgatcttc tgcgcttcgg gtccggtctc
     2761 gaacttgatg aactcccacg ctgccttctg cttcttcgcg tcgtgcgtcg tgatgagcgc
     2821 cgcattgcca ccggtcggaa gcccgccctt cgccttgttg tcgatcggga aggttgtcgt
     2881 gcgcagcgtg aacttgtcgc cgatcaggtc cgtcacctgg cgcagccgtg ccggcgtgtc
     2941 gaagaaaatg ccgatcctgc ctgcgatgaa ctgttgacgc gactggtccc agttgatgag
     3001 cggcatgccg ccttcgctga cgaagcgacg gaagcggtcg agcaccttga cgccgagatc
     3061 gggaccgccg agcggaacgt tcttgccgtc gacgaccgtg ccgccggcct gcaggatcat
     3121 cgcgtgatag agccacacgt cgggccagtc gttcacgttg taggcgatgc cggcgacatc
     3181 cgcaccgccg ctcgccttga ttttcttcgc gagcgccatg atgccgtccc acgtgtcggg
     3241 catgtggtcg ggatcgccgc cggccttctt cacgagctcc gcgttgtaca tcatgatcgg
     3301 cgtcgacgca ttgaacggca gcccgtagag cttgccgtcg accttgccga gcgtgaggat
     3361 ccggtcggag tagttcgcct tccggaactc ggcgccctcg gccgccagca gcgggccgag
     3421 atcgacgatc tgcttgcgct gctcgagcgt gtgggtcagc tcggtcagca ggtggaaacc
     3481 ggagtaatag acgtcgggaa gctggttcgt gaccgcctcg cgcatcatcg tctggtggcc
     3541 gtcgtcgtac gtcggcgcgg gcgcgcggta cttgatcttg atgtccggat ggcgcttcat
     3601 gaattcctgc gcgatcggct cgtggaattt cgcgaacgcc ggaaacgcgt acagcacgtc
     3661 gagcgtcacc tcctccgcgc gcgcgccggt cgccatcacg atcgccgcca gcaggatcga
     3721 acccaggacg ctagccattc tcttcatcgc tgtctccttt cggtggggac gaaatgcatt
     3781 tctcacttga tgccggtcat cgtgatgccc tggatgaagc gccggcgcgc gatcaggaac
     3841 gcgatcacca gcggcgcggt cagcaccgtc gccgccgccg tcagcgcgcc gtagttcgag
     3901 cccgactcgg catcggcgaa gaacatcatg ccaagcggcg gcgtcgccag atgcgtgtcg
     3961 gagatcacga tcagcggcca gtacaggtcg ttccagtgcg cgacgagcga gaacaccgag
     4021 aacgcggcga tcgccggcca tgcgcttggc acgacgatgc gccagacgat ctcgaattcg
     4081 ctcatgccgt cgaggcgcgc ggcatgaatg atctcgtcgg gatagcttct gaagaactgc
     4141 cggaacagaa agatcgcgaa caccgacagg aagaacggca tcatgatcgc gaagtaggtg
     4201 ttcagcaggc cgagcttcgc gagacccatg tacagcggca gcgccggcac ctggatcggg
     4261 atgcaaaggc cgagcagcac cagggcgaac atcgtcgcgc ggccgggaaa gcgcagcttc
     4321 gcaagcgcat acgcacacgg caccgcgatc agcacctgca cgagcaggat cacgccgcag
     4381 acgatgacgc cattcagcgc gaagcgcagc agcggcacct tgccgagcgc gttgccgtaa
     4441 ttctcgaccg cgttgaaatg cttcggccac aggctgaagt cgacggcgaa gatttccggc
     4501 ggcgacttga tcgacgtgac caccatccag acgaacggca gcaggatgaa catcgcgccg
     4561 gcgatcagca caccgtgcac gatcagcagc gtcagcatgc ggcgcacgag cgccgatacc
     4621 ggcgtgatcg cgcgcatgcg ggccgcctcg cccggcagga taagcggcgt cgccatcagt
     4681 agtgcacccg ccggtcgagg acgaacgtct gcaggatcga gaacagcagc acgaacacga
     4741 ggaacaccag cgtcagcgcg gcggcgtacc ccatgtggaa atactggtag ccctcgaggt
     4801 agatcgcgta tagcagcacc tccgacgagc ccatcgggcc accgcgcgtc atgacggcca
     4861 ccgtgtcgaa caccttgaac gcggtgatcg acgtcgtgac gatcacgaac atcgtcgtag
     4921 gtccgagcag cggccaggtg atcgtcagga aacggtcgat gccgcttcgg cagccgtcga
     4981 tctcggccgc ttcgtacagg tcgcgcggaa tcgccgaaag acccgcgagg aagaggacca
     5041 agttgaaacc gacgaggtgc cagatgccga tcgcggcgag tgtcggcagc acgtaggccg
     5101 gctcgctcaa gaaggccatc tcatggaagc ccagcgcgcg cagcatgccg ttgatcggcc
     5161 cgaggctcgg gtgcagcagg aattgccaca ccgtcgccat cgcgatcagc gtcgacgtca
     5221 ccggcaggaa gtacacgacc tcgtagaacg agcgcgtgcg cttgcgcccg tgcagcagca
     5281 ccgcgacgag caggccgagc ccgaccgcga gcggcagcac gatcgcgacg tagacgaacg
     5341 tgttggtgat cgagcggcga aagaccggat cggcgagcgc cttgctgaag ttgtcgaggc
     5401 cgagaaagtg cgtgtccagt gcaccgagcc ggtagtcggt gaagctaagg tagagaagca
     5461 ctgcgagcgg gatgatgtcg atcagcagca gcaaggcgaa cgcgggaaac acgaacccgc
     5521 agccggcgag gcgctcgatc aattgctgcc gattgcgggg cggcagcgca gcgggactcg
     5581 ccattgcagc agccgcggtc atcgaaccgg tacaccgctc atggagacac cgcgctcatc
     5641 ggcagcgcgc ccgcaccgct cgcggcgttc ggcacttccg catcggcgac gcgatgtcct
     5701 tcgtcgtcga acacgtggca cgcggccggc gaaaactgca gcgcgagatc gcgcgcgccg
     5761 tcgacgagcg actcgggatc ggcggcgacc ttgcagatca cgacaccgcg cgcgggctgc
     5821 gcgaggtcga cgtgcagaat atgctcggcg ccgaggtttt cgctgcgatg caggcgcccg
     5881 gcgaggacgt gccggcccgc ggccggcgcg ccgagcggcg ccggcgtgat cgcctccggc
     5941 cggatgccga gcgtcaatgc cgcgcccgcc ggtcgcggca cgcgaatggg cagctcgccg
     6001 ccgaagagct cgaccgaatg ctgcgcgctc acgcgcgcag gcaacaggtt gatcgccggc
     6061 gtgccgatga actgcgcgac cttgacgctt gccgggcgcg cataaagctc gcccggccgg
     6121 ccgagttgca ggatcctgcc gttgtccatc atcgcgacgc gattcgacat cgtcatcgcc
     6181 tcgacctggt cgtgcgtcac gtagatgaag gtcgcgccga gccgcgcatg cagatccgcg
     6241 agctcggtgc gcatgtgcac gcgcagcttc gcgtcgagat tggacaacgg ctcgtccatc
     6301 aggaacgcgg cgggctggcg caccatcgcg cggccgagcg cgacgcgctg gcgctggccg
     6361 cccgagagct gcgacgggcg ccgctcgagc agcgcgtcga tctgcaattg cgcggcgacc
     6421 gcgtgaacct cgcgcgcgat ctcgggcatc acgcgccgtc ttcgcagcga cagcgcgcgc
     6481 aggagcggca gccggtcgaa caggttgagg cgcgacatga cgagcggcaa tgcaatattg
     6541 ccgaacacac gcatgtgcgg ataaagcgcg tagctctgga acaccatcgc cacccgccgc
     6601 tcgtgcggtc gcaggtgatc gaccggcgcg ccgtcgatca gcacgctgcc cgacgattgc
     6661 acttcgaggc cggcgatgat ccgcagcagc gtcgatttgc cacagcccga tgcgccgacc
     6721 agcgtgagga attcaccgtc ggcgatgtcg aggtcgatgc cggaaagcac cgagacatcg
     6781 ccgaacgact tgccgatcgc cctcagttcg acgttcgcca tggctgtgtc gtcagcgacc
     6841 gcccgcgcac atcaggcggc gtgccgcggc ggataaacct cgtgcagcac gtcgtcgatc
     6901 caaagcgtgg tgaggcccgg aatgtccgcc ggcgtgaccg agacgtcggc atcgccaaat
     6961 tcgtagatca ccgcgccgag gcgcttttcg ccgggcatgt cgggctggtt gtcgggtccg
     7021 acgagaaagc ccgacgacgc gcaccagacg tagcggcagc gacccacctt cgtgtcccgc
     7081 catttgtgca gatggccggt ggcgatcagc gcaacgtcat gtcgctcgac gagctcgagc
     7141 agtcgcgcgc gcggcgcggg cttcaccgac caatagccgg tgtccgcttc atcgggcgca
     7201 tcgatgaaca acggccggtg cgtgaaccag gcgatgcgcc gctcgccggc cgtcgccatc
     7261 gtctgcacga ggcattcgaa ttgacgcgcc tcctcgtcgc tgccgtcgcc gaagagcatc
     7321 gagtcgatac cgatcaggcg ccacgcggga agatcgtgcg accagcgatc ggcgccgaaa
     7381 tgccggcgcc agcgatcgag gcgctccgcg ttcaccggct gaaagcgatt cccggcctcg
     7441 ccaacgtcgt gattgccggg gacggcgagc accctcgccg gcagcgcgcg catgagcgct
     7501 gcgccgtcgc gcatgtcctc ctcgctgtcg gcgccgtcga cggtgaggtc gccggtatga
     7561 acgatcaggt cggcatgctg acgcagtgcc cagtcgcgca acggcgccca gttcgcggcg
     7621 aactggcgtt tcgcggggct caggtgcgta tcggagatct gcagaacacg ggccattcgg
     7681 tgctttccat ccgatttcgg gcggattcta tagatgcttt cgcgccgtga caaatgtatt
     7741 ttggtccgcg atatcgctcg gcgcagcgtg cgttcacgcg cggcattcga gcccgtccgg
     7801 tgtcgcgcgg tagccggtaa gcgccttgtc ggcgtgcgcc aaacgccagc tcagcatctt
     7861 ctgcgcatag tcgcgtatcc gcgcggcata gccggggtcc ccggatcgat cgatgaactc
     7921 gttcgggtct tcgcgcagat cgaagaacag cgcgggcagc gcggcaaaat gaacgtactt
     7981 gtaatcggca tcacggacga cggcgagcga gcagcggtcc atcggcaagc ccagcgagga
     8041 ttcgggctgc gaatagtaga cgtcgcgaaa atcgaactcg taatggacct cggttcgcca
     8101 gtcgcgcggc gccgccccgt gacagaagga caggagcgag cggccgtcgc actggcgcgg
     8161 aatcgaaagt ccgagccact cgagcacggt cggcatcgtg tcgacggact cggtgaacga
     8221 gtcgacgatc gtgccgcgcg tgctgttcgc gcgcgggtcc gggtcgctga cgaccatcgg
     8281 aatccgaaag gtctggtcga agtagccgtt cttgccgaga aggtgatgat cgccgagctg
     8341 ctcgccatga tcgcaggtga agacgatcag cgtgtccttc cactgattcg tctcgtcgag
     8401 gaacgcgaaa acgcggccga gatgatcgtc gatctcggtc atgagcccgt aataggccgc
     8461 gcgcatccgg cgcacctgcg cctcgctcat gtcgctcgcg agaccgcgcg cgttcacgaa
     8521 aaaattgctc tgcgcgaggt gccttacata gtacgcaagc agcggatgct ggcgcgcttc
     8581 atcgtccggc gtggcagcgc ggatcggcgg cggcatgtcc gccgggtcgt aatgcgcgtg
     8641 atagggcgcc ggggcgatga acggcgggtg tggccgccag tagccgaggt gcaggaacca
     8701 cggcttgtcg tgcgcgccgc ggaggtaggc gagcgcgcgc tcggtgaagt acgacgtatc
     8761 ggaatcttcg gcggacagca tcgatgatgc cgcgctcgcc cccgggtcgc cggttttcgg
     8821 aagccagatc tcctcccgca cccgcggcaa cgcgaaaccc ctgctcgcga gccacgcgta
     8881 gtacggctcc ttgctcggct cgaagccgcc gacgacgcgg aagccatcca tcacatcgcc
     8941 cagaatgcga aaccggggat cgtgcggcga cgtcgttcgc ggatcgggcg tcgtcgtcgt
     9001 atagccgatc agcgcagggt cgtagccgcc gttgcgcagc tccttgccga gattggtgaa
     9061 gcgcgcgtcg aggggaatcg tattctggac cgcgcggtga ttcatctggt agaggccggt
     9121 cagaagcgat gcgcgggccg ggccgcaggg cgacgtcgtc gtgaaatgat tgcggaacgt
     9181 gacgcccgcg gcgcacaggc ggtcgaggtt gggcgtgcga aggtgcggcg tccccagttt
     9241 cggcaaaagg tcgccgcgcc attgatcgac gacgatcagg agcacgttac gtcgacgccg
     9301 ctcggcggag cgcatgccgt gcctcaggcg gcgtcctgcg cgagcttcac ggcggcacgc
     9361 gcggtcgctt cgtcgcaaac gaacaccgag ccgaggcccg cacgcagcac cgatgcgatt
     9421 gcccggacct tgtgccgccc gccggcggca aagaccaccg tggggatgcg cgacagatcg
     9481 cgcaacggca gggcaatcgc ccgccgattg atcggatggt cgatggcgcg cccgcgctcg
     9541 tcgatgaact gcgcgacgat gtcgcagacg gcgccggccg cacgaagctc cgtcacgctg
     9601 acgtcatcgg gcagcgcgta gcgaacgagc atcgagcgcc gcgtcacgtc gccgacgctc
     9661 agcaccgcga tgtcgctcgt ctcgatgcag cgcagcgtgt cgcggaaaac gtcctgcgcc
     9721 atgatggtgt cgcgcgacct cgggcttccg gcgtacatgg gcgcaaccag gtagcggcat
     9781 tgcgaatgca ggcgccgggc gagctcgctt gcgagatcga acgtgttgaa ctcgagcccg
     9841 tgcgtgaggc cacccatcat cgagttgacg ccgagatcgg gatagcggcc cgcgcgcata
     9901 tggcggatca tttcgcgcag cgtccggccc cagccgatgc cgaggccgcg cgtgccgttg
     9961 ttttcgagat agtgcgaaac gaagcccgcc gccgcccggc cgacgatgac gggaatctgc
    10021 tcgtcgtcgt cgggcgtggg cacgatgacc gccgccttca gcttgaaatc gcggatcatc
    10081 tgctgctcga gctcgacgca cgtcgcaaga tcggcattga gcgtgatgcc gacgagaccg
    10141 ttgcgccgcg cgtcgacgag gatgcggttg atccgaaggc gcgtcgtgcc gagtcggcgc
    10201 gcgatttccg cctgcgtcag gccctccatg aaatacagcc aggcggcgcg gacctcggtc
    10261 tgcttgtcgt cgatcatcgg gcctcggtta gacgcgacgg aggcgtgcag catggtgtcg
    10321 tccccgcgca ggcggggacc cagtgtcgtc ggcacttcat ggcgagcccg gcttcctcgt
    10381 ctcgtaggcg acgatccggg ccaccttgtc cttcgagcgg cccccctgga actcggacgc
    10441 aagaaaggcg tcgacgatcg acttggcgag ctcgctgccc acgacccgcg cgccgatcgt
    10501 gacgatctgc gcgtcgttgc tcattcgtgc gcgtgcggca gaatcggtgt cgtgcgcctg
    10561 cgcagctcgg atgccgggca ccttgttcgc cgagatcgcc atgccgatgc cggtcccgca
    10621 caacaggatg ccgcgatcgt gccggccgga cgcgacggcc tcggcgacgg cgaaggcgat
    10681 atcgggatag agcaccggtg cagcatcgaa cgttccataa tcctgcagct cgacacccgt
    10741 cgcggcaagg tgcttgcgca ggatttcctt gagctcgtag gctgcttcgt cacatccgat
    10801 cgcgatgcgc atcgctcctc cttcgccgcg tgcggcaagc ctgccttgat ttccggatcg
    10861 cggcgtagct taattcgttt gcattgcatc tgcaagcgga cgtttacatt tgcatctttt
    10921 ccggctcccg cggccggccc cggaggttgc acggcgtgcg ctacgtgatc ggaatcgacg
    10981 gtggaaccga gtcgcttcgc gcgcacgtgt tcgacctgca cggcaacagc gccggcagcg
    11041 cgaagggcga ctacgcgacg gccttccccg cccccggtcg cgcggaacag cacccgcgcg
    11101 actggtggcg cgcactcggc gaagccgtcc gcggggcagt cacggccgca ggcatcatcg
    11161 cgcgcgacgt ggcggcgctc gcactcgaca cgacctcggc gacggtggtc gtcaccgacg
    11221 ccgccggtga cccgatgcgg gcggcgatcc tctggatgga cgtgcgcgcc gatgccgaag
    11281 ccgcggcggt gctgcacacg cacgacgacg cgctgcgcgt gaacggcgcc ggtaccgggc
    11341 cggtgtcgcc agaatggatg attcccaagg cgctatgggt gaagcggcac gagcgcgcgc
    11401 tttacgatcg ctgcacgacg atctgcgaat accaggatta catgctgcgc cggctcaccg
    11461 gtcgcaacgt cggcagcctg tcgaacgtcg cgatacgctg gcactatcgc acacgcgaag
    11521 gcggttggcc gcgcacgctg ctcg
//
LOCUS       DASSVX010000258         5557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1144890, whole genome shotgun sequence.
ACCESSION   DASSVX010000258 DASSVX010000000
VERSION     DASSVX010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144890"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFO53_11095"
     CDS             complement(<1..356)
                     /locus_tag="VFO53_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET8876865.1"
                     /translation="MARSPAAARKAPARQTRLLISTRKGLWTLASDASRNGWTLAGPQ
                     FLGHTVHHALTDPRAPRQWLAAARTGHLGPTIFRSQDNGRTWKEAVRPPAFARGSGRV
                     VDHTFWLTPGHADDAGV"
     gene            544..1050
                     /locus_tag="VFO53_11100"
     CDS             544..1050
                     /locus_tag="VFO53_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET8876866.1"
                     /translation="MTIKVGDRLPAGTLMEYHDVEKDGCSIGPNPVSIESLTRGKKVV
                     LFGLPGAFTPTCSAKHVPSYLADYDKLKAKGVDTIACMSVNDAFVMGAWGRDLHADDK
                     VRMLADGSAAYTKALGLELDLTARGMGVRCQRFSMLVDDGVVRTLNVEAPGKFEVSGA
                     ETMLKQLG"
     gene            complement(1126..2004)
                     /locus_tag="VFO53_11105"
     CDS             complement(1126..2004)
                     /locus_tag="VFO53_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET8876867.1"
                     /translation="MIRPKGLGRGLDALLAGNDEAAASGESLQTLSVDRLRPGKYQPR
                     AKMDAASLAELALSIKEQGVMQPILVRPVDGGVFEIVAGERRWRAAQQAGLREVPALV
                     KNVPDQAALAVALIENIQREDLNPLEEARGLARLIDEFGLTHDAAAKAVGRSRSAVSN
                     LLRLNALAPPVQEYLLAGQIEMGHARALLALPVAQQSGSAARVVSDALTVRDTERLVH
                     ALLNPAAIARRHKAKRPLDADTARLENDLGERLGAVVRIEPGRRGAGRVVIRYASLEQ
                     LDGIIERLGPTVHSPA"
     gene            complement(2057..2917)
                     /locus_tag="VFO53_11110"
     CDS             complement(2057..2917)
                     /locus_tag="VFO53_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8876868.1"
                     /translation="MTRIIAVVNQKGGVGKTTTTVNLAASLVAMKRRVLVVDLDPQGN
                     ATTGAGIDKRARKRTVYHVLLGEASIGDVRVHSESGGFDIVPSNRELAGAEVELVDLP
                     ERETRLKDALAEALRQMREALDAIAADYDFVFLDCPPSLSLLTLNGLCAADSVLIPMQ
                     CEYYALEGLSDLVQTVKKVRAHLNPKLEIEGLLRTMYDPRNTLAQNVAAELERHFGER
                     LYRTIIPRNVRLAEAPSHGVPALKLEAKSKGALAYLALAGEVVRRMESRQRALTHVQT
                     ATTTDVAATE"
     gene            complement(2914..3579)
                     /gene="rsmG"
                     /locus_tag="VFO53_11115"
     CDS             complement(2914..3579)
                     /gene="rsmG"
                     /locus_tag="VFO53_11115"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017509497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET8876869.1"
                     /translation="MTPDAQRETQGEAARPAIVPIAQSLPAGARSKLSAYLDLLAKWN
                     RVYNLTAIRDRTQMQSLHVDDALAVLRFLPDAPHLRLLDVGSGGGVPGIPLAISQPGW
                     DVVLLDSNRKKVSFLTQAAIELRLDNVQPVASRIEDYVCNDRFDVVISRAFSDLASFA
                     RGARAHVAPGGVIVAMKGVLPQDEIEALPGDIGVVATPALDVPGLDAERHLVIMQPKG
                     GPA"
     gene            complement(3576..5492)
                     /gene="mnmG"
                     /locus_tag="VFO53_11120"
     CDS             complement(3576..5492)
                     /gene="mnmG"
                     /locus_tag="VFO53_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET8876870.1"
                     /translation="MKHSERFDVIVVGGGHAGTEAALAAARIGRRTLLLTHSLETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMAIATDEAGIQFRTLNGSKGPAVRATRAQADRV
                     LYRGAIRLRLENAPNLTLFQQPVDDLLLAGDRIRGVATQIGIEFEADAVVLTTGTFLS
                     GLIHVGLQNYEAGRAGDPPAKRLGARLRELALPMGRLKTGTPPRLDGRTIDFSNLTVQ
                     PGDDPAPIFSFMGARDMHPAQRVCWITHTTERTHEVIRGGLDRSPLFTGVIKGTGPRY
                     CPSIEDKVVRFAHRASHQIFLEPEGLDTTEIYPNGISTSLPFDVQLALVRSIPGCEQA
                     HIVRPGYSIEYDYFDPQGLRSTLETRAIGGLFFAGQINGTTGYEEAAAQGILAGINAA
                     RYAGGEEGWCPRRDEAYVGVLVDDLITRGVSEPYRMFTSRAEYRLQLREDNADLRLTE
                     HGRRLGVVDDKRWGAFCRKRDAVARERERLKSTYLSPQVVARHDAERVLGQPLERERS
                     LFDLLRRPSVGYDALHELAGAGTPVADAAVAEQVEIATKYEGYIDRQDAEIARQAGQE
                     STRLPANLDYRSVRGLSIEVQQKLTASQPETLGQAARISGVTPAAISLLLVHLKRGFP
                     GGAASGADAASCGS"
BASE COUNT          909 a         1862 c         1893 g          893 t
ORIGIN      
        1 acgcccgcgt cgtccgcgtg ccccggcgtc agccagaacg tgtgatcgac gacgcggccg
       61 ctgccgcgcg cgaacgccgg cgggcgcacc gcttccttcc acgtgcggcc gttgtcctgc
      121 gaacggaaga tcgtcggtcc gaggtgcccg gtccgcgccg ccgcgagcca ctggcgcggt
      181 gccctcggat cggtgagtgc gtggtggacc gtatgtccga gaaactgcgg gccggcgagc
      241 gtccagccgt tgcgagaagc gtcgctcgcg agcgtccaca acccctttcg ggtcgagatc
      301 aaaagccggg tctgtcgcgc gggcgccttg cgcgccgcgg cgggggaacg tgccatcgag
      361 ccctccttcg gttcacgcct gtcgggttcg ctgcccgagg cgaaagggtg gaaccctacg
      421 ctgcgcgagc gacagcggca agatgcgccg gcgggcgtgg gggttgaaat tgcggcatcc
      481 ggctgcgatc ctcgcatatc cacccgccgt ccgtcgggac ggccttccca ggagaaaagc
      541 gccatgacga tcaaggtcgg agaccggctg cctgcaggta cattgatgga ataccacgac
      601 gtcgagaagg acggctgctc gatcggcccc aacccggtca gcatcgaaag cctgacgcgc
      661 ggaaaaaagg tcgtgctgtt cggcttgccg ggcgcgttca cgccaacgtg ctccgcgaag
      721 cacgtgccga gctaccttgc cgactacgac aagctgaagg cgaagggcgt cgacacgatc
      781 gcgtgcatgt cggtcaacga cgcgttcgtc atgggcgcgt ggggccgcga cctgcacgcg
      841 gacgacaagg tgcggatgct ggccgacggg agcgccgcct acacgaaggc gctcggtctc
      901 gagctcgacc tcaccgcgcg cggcatgggc gtgcgctgcc agcgcttttc gatgctggtc
      961 gacgacggcg tcgtcaggac tctcaatgtc gaagcgcccg gcaagttcga ggtgtccggc
     1021 gccgagacga tgctgaagca actcggctga gcgtcgcgcc gcacggcatc ggcaagatcg
     1081 ccggcgtgcg gcgcgatgcg ccgccccggc gccatcgcgt ggcgatcacg caggcgaatg
     1141 caccgtcggg ccgagccgct cgatgattcc gtcgagctgc tcgaggctcg cgtagcggat
     1201 cacgacgcgg cctgcgccgc ggcggcccgg ctcgatgcgc acgacggcgc cgaggcgctc
     1261 gccgagatcg ttctcgagcc gtgcggtgtc cgcatcgagc gggcgcttcg ccttgtggcg
     1321 acgcgcgatg gccgccggat tgagaagggc gtgcacgagg cgctccgtgt cgcgcaccgt
     1381 cagcgcgtcg ctcaccacgc gcgcagcgct acccgactgc tgcgccacgg gcaaggcgag
     1441 gagcgcgcgc gcatggccca tctcgatctg tccggcgagc aggtattcct gcaccggcgg
     1501 ggcgagcgcg ttgaggcgca gcagattcga aaccgcgctg cgcgagcgtc ccacggcctt
     1561 cgccgcggcg tcgtgcgtaa gcccgaactc gtcgatcagc cgcgccaggc cgcgcgcctc
     1621 ctccagcgga ttcaagtcct cgcgctggat gttctcgatc agcgcgacgg cgagcgccgc
     1681 ctgatcggga acgttcttca cgagcgccgg aacctcgcgc aatcccgcct gctgcgccgc
     1741 gcgccagcgc cgctcgccgg ccacgatctc gaacacgccg ccgtcgaccg gacgcacgag
     1801 gatgggctgc atcacgccct gctccttgat cgacagcgcg agttcggcga gcgaggcggc
     1861 gtccattttc gcgcgcggct gatacttgcc gggccggagc cggtcgaccg acagcgtctg
     1921 cagcgattcg cccgacgccg cagcttcgtc gttgcctgca agaagcgcgt cgaggccgcg
     1981 gcccagtccc ttcggtcgga tcatcgttgg ctccgattac gccgtccgga cgctgcccgc
     2041 cggcgaactg atgtggtcat tcggtggccg cgacgtcagt ggtggtggcc gtttgaacat
     2101 gagtgagtgc tcgctgcctg gattccatgc gacgcacgac ttcgccggcc agcgcgagat
     2161 acgcgagcgc ccccttcgac ttcgcctcga gcttgagcgc cggcacgccg tgcgacggcg
     2221 cttcggccag gcgtacgttg cggggaatga tcgtgcggta aagccgctcg ccgaagtggc
     2281 gctcgagctc ggcggcgacg ttctgcgcca gcgtgttgcg cggatcgtac atcgtgcgca
     2341 gcaatccctc gatctcgagc ttcggattca agtgagcacg cactttcttg acagtctgca
     2401 ccagatccga caatccctcc agcgcgtagt actcgcactg catgggtatc agtaccgaat
     2461 cggcggcgca aaggccgttg agcgtcagca gcgacaagga cggcgggcaa tcgaggaaaa
     2521 cgaagtcgta gtcggcggcg atggcgtcga gcgcctcgcg catctggcgc aaggcctcgg
     2581 ccagcgcatc cttgagccgc gtctcgcgct cgggcagatc gacgagttcg acctcggccc
     2641 cggccagttc gcgattcgat ggcacgatgt cgaagccgcc gctttcggaa tgcacgcgga
     2701 catcgccgat gctcgcctcg ccgagcagca cgtgatagac ggtgcgcttt cgtgcgcgct
     2761 tgtcgatgcc ggcgcccgtg gtcgcgttgc cctgcgggtc gaggtcgacg accagcacgc
     2821 ggcgcttcat cgccacgaga cttgcggcga ggttgacggt ggtcgtcgtc ttccccacgc
     2881 cgcctttctg gttcaccacc gcaatgatgc gcgtcacgca ggaccgcctt tcggttgcat
     2941 gatgacgagg tggcgctccg catcgagccc cggcacgtcg agtgccggcg tcgccacgac
     3001 gccgatatcg cccggcagcg cctcgatctc gtcctgcggg agcacgccct tcatcgccac
     3061 gatgacgccg cctggcgcaa cgtgcgcgcg cgcaccgcga gcgaagctcg cgaggtcgga
     3121 aaaggcgcgg gagatcacga cgtcgaagcg gtcgttgcag acgtaatcct cgatgcgcga
     3181 cgcgaccggc tgcacgttgt cgagccgaag ctcgatggcg gcctgcgtga gaaagctcac
     3241 cttcttccgg ttcgagtcga gcagaacgac gtcccagccg ggttgcgaga tggcgagcgg
     3301 aattccgggc acgccgccac cggagcccac gtcgagcagc cgcaggtgcg gtgcatcggg
     3361 caggaagcgc agcacggcga gcgcatcgtc gacgtgcagc gactgcatct gcgtgcggtc
     3421 gcgaatggcg gtcaggttat acacgcgatt ccatttggcc agcaggtcga gataggccga
     3481 cagcttcgag cgtgcgcccg caggaagcga ttgcgcaatc ggcacgatgg ctggccgcgc
     3541 tgcttcgccc tgcgtttcgc gctgtgcgtc cggcgtcatg acccgcacga cgcggcgtcg
     3601 gcgccggaag cggcgccgcc gggaaagccg cgcttgagat ggacgaggag cagcgaaatc
     3661 gccgccggcg tgacgcccga aatgcgcgcc gcctggccga gggtctccgg ctggcttgcg
     3721 gtcagctttt gctgcacctc gatcgataga ccgcgtaccg aacggtagtc gaggttcgca
     3781 ggcaaacggg tcgattcctg ccctgcctga cgcgcgatct cggcgtcctg gcggtcgatg
     3841 tagccctcgt attttgtcgc aatctcgacc tgctcggcca cggcagcgtc ggcaaccggc
     3901 gttccggcgc ccgccaactc gtgcaatgca tcatagccaa cgctcgggcg gcgcaacagg
     3961 tcgaacagcg accgctcgcg ctcgagcggc tgcccgagca cgcgctccgc gtcgtggcgc
     4021 gcaacgacct gcggcgacag atacgtcgac ttcaagcgct cgcgttcccg cgcgaccgcg
     4081 tcccgcttcc ggcaaaaggc accccaacgc ttatcgtcga cgacgccgag gcgccggcca
     4141 tgctcggtca ggcgcaggtc ggcgttgtcc tcgcgcaact gcaaacgata ttcggcacgc
     4201 gaggtgaaca tccggtaagg ctcggagacg ccgcgcgtga tcaggtcgtc gaccaggacg
     4261 cccacgtaag cctcgtcgcg ccgcggacac cagccctcct cgccgccggc gtaccgggcg
     4321 gcattgatgc cggcgagaat gccctgcgcc gcggcctctt cgtagccggt cgtgccgttg
     4381 atctggccgg cgaagaacag gccgccgatc gcgcgcgtct cgagcgtcga acgcaggccc
     4441 tgcggatcga aataatcgta ttcgatcgaa tagccggggc gaacgatatg cgcctgctcg
     4501 caacccggaa tcgatcgcac gagcgccagc tgcacgtcga acggcagcga cgtggaaatg
     4561 ccgttcggat agatctccgt cgtgtcgagc ccctcgggtt cgaggaagat ctgatgactc
     4621 gcccggtgtg caaaacgcac gaccttgtct tcgatcgaag ggcagtagcg cggacccgtg
     4681 cccttgatca cgccggtgaa aagtggcgac cggtcgaggc cgccgcggat gacctcatgg
     4741 gtgcgttcgg tcgtgtgcgt gatccagcac acacgttggg ccggatgcat gtcgcgggcg
     4801 cccatgaagg aaaagatcgg tgccgggtcg tcgccgggct ggacggtcag gttggagaag
     4861 tcgatcgtgc gtccgtcgag gcgcggcggc gtacccgttt tcagccgccc catcggcagc
     4921 gccagctcgc gcaggcgggc gcccaggcgt ttggctggcg ggtcgcccgc gcgtcccgcc
     4981 tcgtaattct gcaggcccac atggatcaac ccggacagga aggtgccggt agtcagcacg
     5041 accgcatcgg cctcgaactc gatgccgatc tgggtcgcga caccgcgaat gcgatcgccc
     5101 gcaagcagca ggtcatcgac aggctgctgg aagagcgtga gattcggcgc gttctccagt
     5161 cggaggcgga tagccccgcg ataaagcaca cggtcggctt gtgctcgcgt tgcccgtacc
     5221 gccgggccct tgctgccgtt gagcgtacgg aactggatgc ccgcttcgtc ggtggcaatg
     5281 gccatcgccc cgcctaatgc atccacctcc ttgacgagat gccccttgcc aattccgcca
     5341 atggacggat tgcaggacat ctgtcccagc gtttcgagac tgtgcgtgag caatagcgtg
     5401 cggcgaccga tgcgggccgc agccaaggcc gcctcggtac cggcgtgacc gccaccgacg
     5461 acgatgacgt cgaatcgttc ggagtgtttc aaggaaagag gcggcgcgga gcgaaagaac
     5521 ttcgcaattc taccgtgtgc gacgtttcac gtgaaac
//
LOCUS       DASSVX010000259         5743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_178505, whole genome shotgun sequence.
ACCESSION   DASSVX010000259 DASSVX010000000
VERSION     DASSVX010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178505"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFO53_11125"
     CDS             <1..186
                     /locus_tag="VFO53_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876871.1"
                     /translation="DDFPRQLAAARIAVPAATFVVTEAAGESEQVQDALAKFALRAPA
                     VAVEEVEANSENSSTNR"
     gene            complement(198..725)
                     /locus_tag="VFO53_11130"
     CDS             complement(198..725)
                     /locus_tag="VFO53_11130"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET8876872.1"
                     /translation="MDIKAMIRTIADFPKPGVQFRDITTLLKTPGGLREVVDRLFAEY
                     ANERVDKVAAIESRGFIVGGPLAYRLGAGFVPIRKKGKLPAENFGEDYVLEYGCDRLE
                     VHCDALAHGERVLLVDDLIATGGTAEAATRLVRTAGASLIGCAFVIDLPDLGGRRRLE
                     ALGYRVRALCQFSGH"
     gene            complement(794..1609)
                     /locus_tag="VFO53_11135"
     CDS             complement(794..1609)
                     /locus_tag="VFO53_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HET8876873.1"
                     /translation="MTPLVTSFRMYNATSAVAAAWRALFERVFAAVAADVHFVEHRYP
                     QPIASLWSEPGLCAAFMCGWPFVRSKGAMQAIAAPVPSPARYAGLPRYCSEFLVREAS
                     GWTALDDTFGHRIGSMPPDSQSGFNAPRALLARYVSERRRTLYRESRGPYMTPAKLLD
                     ALRTNEVDVVALDGFYLDLVRHSEPAMLADVRTVATTPWTPIPLLVAAPGVDPDLVAR
                     LREHLLALHENSASAACLRAVALAHFVVPDLASYARLPEMARFAIERGYDAIG"
     gene            complement(1656..2663)
                     /locus_tag="VFO53_11140"
     CDS             complement(1656..2663)
                     /locus_tag="VFO53_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HET8876874.1"
                     /translation="MTATIDITYLNARDVEQLALTDDEILAAVESALAAQGRRETVIE
                     PRVHLVPESPASGHFNILRGYVKPLGVAGVKIVGDFVGNYALGLPSEMALLNLFDPRT
                     GMPLAVIDATAITDMRTGAMTAIGARHLARRGSKVLGHVGTRGTAYWNVRLLDRLFDF
                     AEIRVHSRRAESRQAFADRLAHDLGKPVQVTSDWQSCVQDADIVVEASRLPEPFAHLK
                     TAWITKGALVIPYGTMSAVELSLTDIMDKIVVDDWGQCRKGLPFGALRQHVDSDRLTE
                     HNLHAELGDIVAGTKPGRERDDETILFWHRGLGTTDIALGHALLQKARSAAVGQTLRF
                     M"
     gene            complement(2674..3651)
                     /locus_tag="VFO53_11145"
     CDS             complement(2674..3651)
                     /locus_tag="VFO53_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET8876875.1"
                     /translation="MKHRVQRGLLAFATLLLACPLAVAQDAWPTKPVVIIVPFAAGGV
                     ADLLPRIVGAKLSEKWKQPVIIENKVGASGNIGMAEGAHAAPDGYTFVLAPAGNLTVN
                     PMLFKHLTFDTYKDLTPVTNLAQVPNVLVVHPSVPAKTFGELIAYAKANPGKLNFASP
                     GAGSGAHLAGELLKVDAGIDMVHVPYKGLAPAVNDLLGGNTQMMFAGISTVIQYVKTG
                     KLVALAIASPKRSPELPDVPTVAESGLPGFEVTSWYGIVTRAGTPASIIAKVQRDMAE
                     ALRQDDVRAKLAGAGLEPVGSSPDEFAAMIKAETRKWGDIVRKANITVD"
     gene            3785..5407
                     /locus_tag="VFO53_11150"
     CDS             3785..5407
                     /locus_tag="VFO53_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET8876876.1"
                     /translation="MRTRGADALMRVLRERGVSQVFTVSGNHVMSAFDAALDAGVALV
                     HARHEAAAVHMADARSRVSGQVGVALVTGGPGHANAISALYTARMAEAPVVLLSGQAP
                     RSQLGMGAFQEMAQAELALPLTKAAWTCSGPDAIARDVARAIAIACAGRPGPVSLSLP
                     SDALEGDAGSGFDHEAAAAPETLDPAVADAIVERLRKAARPIVIVGPASMTRHGRVGA
                     TALEAAIGIPVVGMESPRGIADPSLGAFGEMLAQADCVVLVGKRLDFTLRFAKPPAIA
                     ADCTFLQIDADAEEFARTRRAVGDRLALTARAAAAATLQRLAERASARAPSRHAVWQR
                     EVEAAIAYRPPEWKGASSKDPHRLHPLEALRPLQALLDSRPDSVFVSDGGEFGQWAQA
                     CLHAPHRVINGMAGAIGSALAYAIGARCALPDAPIVATLGDGTFGFHAAEIDTAARYK
                     LPFVAVVGNDARWNAEYQIQLREYGAGRLVGCELAPTRYDAVCAAFGGYGELVTRASD
                     MVGAISHAHASKAPACVNAMIEGVAAPTFARR"
BASE COUNT          948 a         1915 c         1927 g          953 t
ORIGIN      
        1 gacgatttcc cgcgacagct cgcggcggcc cgtatcgcag tccctgccgc cacgttcgtc
       61 gtaacggagg ccgccggcga gagcgagcag gtccaggacg cactcgccaa gtttgcgttg
      121 cgggcgccgg ccgttgccgt agaggaagtt gaagcaaact ctgagaatag ctctacaaac
      181 cgctgatata aataagctca atgtcctgag aactgacaca gtgcacgcac ccggtacccc
      241 agcgcctcca gcctgcggcg gccgccgaga tccggcaggt cgatgacgaa cgcgcaaccg
      301 atcagcgacg cacccgccgt gcggacgagc cgcgttgctg cctcggcggt accgccggtc
      361 gcaatgagat cgtcgacaag cagcacgcgc tcgccgtgcg cgagtgcgtc gcaatgcact
      421 tcaaggcggt cgcaaccgta ctccagcaca tagtcctcgc cgaagttttc ggccggcagc
      481 ttgcccttct tgcgaatcgg aacaaacccg gcgccgaggc ggtacgcgag cggaccgccg
      541 acgatgaatc cgcgcgattc gatggccgcc accttgtcga cgcgctcgtt cgcgtactcg
      601 gcgaaaagcc ggtcgacgac ttcgcgcagc ccgcccggcg tcttcagcag cgtcgtgatg
      661 tcgcgaaact ggacgccggg cttcggaaag tccgcgatcg ttcggatcat cgccttgatg
      721 tcgatttcgc tcgccatggt tctccgttac gttcgattgg cgcaaccgct tcgcgcggcg
      781 cgtcgcggtt tcctcagccg attgcgtcgt agccgcgctc gatcgcgaac cgcgccatct
      841 ccggcaatcg tgcatacgac gcgagatcgg gaaccacgaa gtgggcgagc gcgactgcgc
      901 gcaggcacgc ggccgacgcg ctgttctcgt gcagtgccag caggtgctcg cgcaagcgcg
      961 caacgaggtc gggatcgacg ccaggtgcgg cgacaagcaa cggaatgggc gtccacggcg
     1021 tcgtcgcaac cgtgcgcacg tccgcgagca tcgccggctc ggaatggcgg acgagatcga
     1081 ggtagaagcc gtcgagcgcg acgacgtcga cttcattcgt gcgcaacgcg tcgagcagct
     1141 tcgcgggcgt catgtacggg ccgcgcgatt cccgatacag cgtgcgccgg cgctcgctga
     1201 cgtagcgcgc gagcagtgcg cgtggcgcgt tgaagccgga ttgcgaatcg ggcggcatcg
     1261 acccgatgcg atgtccgaac gtgtcgtcga gcgccgtcca tccgctcgct tcgcgcacca
     1321 ggaactcact gcaatagcgc ggcagtcccg cgtagcgagc gggggagggg accggcgctg
     1381 cgatcgcctg catggcgccc ttcgaacgca cgaagggcca tccgcacatg aacgccgcgc
     1441 aaaggcccgg ctcgctccac agcgaggcaa tgggctgcgg gtaccgatgc tcgacgaagt
     1501 gcacgtcggc ggcgacggcg gcgaagacgc gctcgaacaa cgcgcgccac gcggcggcca
     1561 cggccgacgt cgcgttgtac atgcgaaacg acgtgacgag tggtgtcacg gcgcacgttc
     1621 gccgcggccg tggtgtcgtg tcgccatggc gcgcatcaca tgaaccgcag cgtctgtccg
     1681 acggccgcgc tgcgcgcctt ctgcaacaac gcgtggccga gcgcgatatc agtggtgccg
     1741 agtccgcgat gccagaacag gatcgtttcg tcgtcgcgct cacggcccgg cttcgttccg
     1801 gcaacgatgt cacccagttc ggcgtgcagg ttgtgctcgg tgagccggtc gctatcgaca
     1861 tgctgacgta gcgcgccgaa cggcagtccc ttgcggcatt ggccccagtc gtcgacgacg
     1921 atcttgtcca tgatgtcggt gagcgaaagc tcgacggcgc tcatcgtccc gtacggaatc
     1981 accaacgcgc ccttcgttat ccatgccgtc ttcagatgcg cgaacggctc gggcagccgc
     2041 gacgcctcga cgacgatgtc ggcgtcctgc acgcacgatt gccagtcgct cgtgacctgc
     2101 accggcttgc cgagatcatg cgcaagccga tcggcgaacg cctgccggct ctcggcgcgc
     2161 cgcgaatgaa cgcgaatctc ggcgaaatcg aagagccggt cgagcaggcg cacgttccag
     2221 tacgccgtgc cccgcgtacc gacatggccg agcaccttgc tgccgcggcg cgcgaggtgt
     2281 ctcgcgccga tcgcggtcat cgcgcccgtg cgcatgtcgg tgatcgcggt cgcgtcgatc
     2341 acggccagcg gcatgccggt gcgcggatcg aacaggttga gcagtgccat ttccgacggc
     2401 agtcccagtg cgtagttgcc gacgaaatcg ccgacgatct tcacacccgc cacgccaagc
     2461 ggcttcacgt agccgcgcag gatgttgaag tgcccgctcg ccgggctttc cggaacgaga
     2521 tgcacgcgcg gctctatcac cgtctcgcga cgaccctgcg cggcgagcgc cgattcgacc
     2581 gccgcaagga tctcgtcgtc ggtcagcgcg agttgctcga catcccgtgc attgagatac
     2641 gtgatgtcga tcgtcgccgt catcgcgccg cgctcagtcg accgtgatgt tcgccttgcg
     2701 cacgatgtct ccccacttgc gcgtctcggc cttgatcatc gcggcgaact cgtccggact
     2761 gctcccgacc ggctcgaggc cggcacccgc cagtttcgcg cgcacgtcgt cctgccgcag
     2821 cgcttccgcc atgtcgcgct gcactttcgc gatgatcgac gccggcgtac cggcgcgcgt
     2881 cacgatcccg taccacgacg tcacttcgaa cccgggcagg ccgctctcgg cgaccgtcgg
     2941 cacgtcggga agctcgggcg agcgtttcgg gctcgcaatg gcgagcgcca ccagcttgcc
     3001 ggtcttcaca tactggatga cggtggagat gccggcgaac atcatctgcg tattgccgcc
     3061 aagcagatcg ttgacggccg gggccagccc cttgtacggc acgtgcacca tgtcgatgcc
     3121 tgcgtccacc ttcagcaatt cgccggcgag gtgcgcaccg ctgccggcac cgggcgacgc
     3181 gaaattgagc ttgcccgggt tcgccttcgc atacgcgatg agctcgccga acgtctttgc
     3241 gggaaccgaa ggatgcacga cgagaacgtt cggcacctgc gcgaggttgg tgaccggcgt
     3301 gaggtccttg tacgtgtcga acgtcaggtg cttgaacagc atcggattga cggtcagatt
     3361 gcccgccggt gcgagcacga acgtgtagcc gtcgggcgcc gcgtgcgcgc cttccgccat
     3421 gccgatgttg cccgaggcgc cgaccttgtt ctcgatgatg acgggctgct tccacttctc
     3481 ggaaagcttc gcgccgacaa tgcgcggcag caggtccgcg acgccgcctg cggcgaacgg
     3541 cacgatgatg acgaccggct tcgtcggcca tgcgtcctgc gcaacggcga gcgggcacgc
     3601 gagcaggagg gtggcgaacg ccagcaggcc gcgctgaaca cgatgcttca tctgaattcc
     3661 ctcttcgaaa cgcccttggg cgacgccgcg catgatgtcg caattccgcg cgtggcgcca
     3721 tcgtatgcga tgatcgacga tccgcttttt tagctgcgtt cgtcatgttg cagccgagtc
     3781 gctcatgagg acccgcggcg ccgatgcgct gatgcgcgta ctgcgcgagc gcggcgtgtc
     3841 gcaggtgttc accgtatcgg gcaatcacgt catgtcggcg ttcgacgcgg cgctcgatgc
     3901 cggcgtggcc ctcgttcacg cgcgccacga ggcggcggcc gtgcacatgg ccgacgcgcg
     3961 ctcgcgcgta tcggggcagg tgggtgttgc gctcgtcacc ggcgggcccg gtcacgccaa
     4021 cgcgatctcg gcgttgtaca cggcgcggat ggcggaagcg ccggtcgttc ttctgtccgg
     4081 ccaggcaccg cgaagtcaac tcggcatggg cgcgttccag gagatggcgc aagcggaact
     4141 cgcgctgccg ttgaccaagg ccgcgtggac atgcagcggg ccggacgcaa tcgcacgcga
     4201 tgtcgcgcgt gcgattgcca ttgcgtgcgc aggacggccg gggccggtca gtctcagcct
     4261 gccgtcggac gcgctcgagg gcgatgcagg atcgggtttc gatcacgagg ccgcggccgc
     4321 gcccgagacg ctcgatccgg ccgtcgccga tgcgattgtc gagcgcctgc gcaaggcagc
     4381 gcggccgatc gtcatcgtcg gaccggcgag catgacgcga cacggtcgcg tcggtgcgac
     4441 cgcgctcgaa gccgcaatcg gcatcccggt cgtcgggatg gaaagcccgc gcggcattgc
     4501 cgatccgtcg ctcggcgcat tcggcgaaat gcttgcgcaa gccgattgcg tcgtgctcgt
     4561 gggcaagcgg ctggacttca cgcttcgatt cgcgaagccg ccggcgatcg cggccgattg
     4621 cacgtttctc cagatcgacg cggatgcgga ggaattcgcg cgaacgcgtc gcgcggtggg
     4681 cgatcgtctc gcgttgaccg cacgagccgc cgcggccgca acgctgcagc ggctcgccga
     4741 acgcgccagc gcgcgggcac ccagccggca cgcggtctgg cagcgcgaag tggaagccgc
     4801 gatcgcgtac cggccgccgg agtggaaagg cgcctcgtcg aaagatcccc atcgcctgca
     4861 tccgctcgag gcgctgcgtc cgctgcaggc gctgctcgac agtcgtcccg attcggtgtt
     4921 cgtctccgac ggcggtgaat tcggccagtg ggcacaggcg tgcctgcacg cgccgcatcg
     4981 ggtgatcaac ggaatggcgg gcgccatcgg gtcggcgctg gcgtatgcga ttggcgcgcg
     5041 ctgtgcgctc cccgatgcgc cgatcgtcgc aacgctcgga gacgggacgt tcggctttca
     5101 cgccgcggaa atcgataccg ccgcacgcta caagctgccg ttcgtggccg tcgtgggcaa
     5161 cgacgcgcgc tggaacgccg aatatcagat ccagcttcgc gagtatggcg ccgggcgtct
     5221 cgtcggatgc gagctggcgc cgacgcgcta cgacgcggtg tgcgcggcgt tcggcggcta
     5281 cggtgagctc gtcacgcggg cctccgacat ggtcggcgcg atctcgcatg cgcacgcctc
     5341 gaaggcgccg gcgtgcgtga acgcgatgat cgaaggcgtg gcagccccga cgttcgcgcg
     5401 ccgctagccg gcgtcacgga ctgaccggga cgccgacgtc agtgcgacat gaagtaaaac
     5461 atcaacaata gcccagatca gactgataaa taaacaccta tcaccaaatt ccggagtgcg
     5521 taaaccggac ggcgcacgag acagaggggt cgttgcgtgc gcttcggtgt tccgttgtga
     5581 catttgacat aataacaatt atacgaagta cgacaggcat cgagtccggg ccgctagggc
     5641 aagatgcaac cgagcccacg gaggctcccg tggcttcgcc cgcagacggc ggcgcgcggt
     5701 tttcagtgcg tgctgcgtga ccgctgctcg ttcgcgcctt cct
//
LOCUS       DASSVX010000260         9399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1028122, whole genome shotgun sequence.
ACCESSION   DASSVX010000260 DASSVX010000000
VERSION     DASSVX010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028122"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VFO53_11155"
     CDS             <1..837
                     /locus_tag="VFO53_11155"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HET8876877.1"
                     /translation="YENVAQCAGGSASTTGFDDGPPLVTGAQIGDSGTGLHLALGIVC
                     ALYQRKTTGRGQKVNAAMQDGVLNLCRVKLRDQQRLDRTGVLEEYPQYPNGTFGEAVP
                     RAGNASGGGQPGWILKCKGWENDPNAYIYFITQAPVWKDICKVIGREEWLTDPRYATP
                     KGRLPHLSEIFDEIEKWTMTKTKFEAMELLNEYDIPCGPILSMKEIAEEPSLRSTGTV
                     VEVDHPKRGKYLTVGNPIKLSDSPTDVKRSPLLGEHTDEVMKELGYSPAQIEELRSQR
                     VI"
     gene            846..2972
                     /locus_tag="VFO53_11160"
     CDS             846..2972
                     /locus_tag="VFO53_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET8876878.1"
                     /translation="MAHDKTAVRSIIDKVKAEGRTSLTAPEGKLVCDAYGIPVPQEGV
                     AKSASEAVRLASAMGFPVVLKIVSPEILHKTEAGGVLVGMKDAAAVEQGYATIMANAK
                     KYNGKANLVGVQVQQMLTGGQEVIIGAVSDPSFGKLVAFGLGGVLVEVLKDVTFRLAP
                     ASHDDALAMLDGIAAAEMLKGVRGSEPVNREALALMIERVSQLASDFPEISELDLNPV
                     FATPKGAIAADVRIVIGAPMPAKYRPSQQDIVKAMNRIMKPDSVAVIGASPEAGKIGN
                     SVMKNLINGGYQGKIYPIHPKADEILGYKAYRSVKDVPADIDVAVFAIPAKFVAQALT
                     EVGEKGIPGAVLIPSGFAETGNVEGQQEIQEIGRKYNVRLMGPNIYGFYYTWKNLCAT
                     FCTPYDVKGHTALSSQSGGIGMAIIGFSRSAKMGVSAIVGLGNKADLDEDDLLTFFEQ
                     DDNTHIIAMHCEDLKDGRSFAEVAKRVSKKKPVVVLKAGRTAMGARAASSHTGALAGN
                     DKIYDDVLRQSGVIRAPGLRDMLEYARAIPLLATPAGENVVIITGAGGSGVLLSDAVV
                     DNGLKLMAMPQDLDAAFRKFIPPFGAAGNPVDITGGEPPTTYKNTIKLGLTDPRIHAI
                     ILGYWHTIITPPMVFANLVVEVIEEMKAQGIHKPVVASLAGDVQVEEAAEFLFQHGVP
                     AYPYTTEMPVAVLGAKYKWARGAGLL"
     gene            3158..4165
                     /locus_tag="VFO53_11165"
     CDS             3158..4165
                     /locus_tag="VFO53_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET8876879.1"
                     /translation="MSHIRRRALGVALGVFALAAVSSAPVAAWEPTKPIQFIVPAGTG
                     GGADQMARFIQGVASKHKLLKEPIIVVNESGGAGAQGFLDVKGSKGDPNTIIITLSNL
                     FTTPMATGVPFSWKDLTPVAMLALDEFVLWVNADTPYKTATEYINAVKAKPGTFKMGG
                     TGSKQEDQIITAQIEQDTGAKFIYIPLKGGGDVAVQLVGGHIDSSVNNPIEAVAQWRA
                     GKLRPLCVFDSKRMDYKEKVTQTMSWNDIPTCKESGLPVEYLMLRGIFMPAGVTQDQV
                     NFYIDLFKKIRATPDWKEFSEKAAFNQSFMTGQEFVSWVTQAENQHKALMEKAGFMAK
                     K"
     gene            4292..4816
                     /locus_tag="VFO53_11170"
     CDS             4292..4816
                     /locus_tag="VFO53_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HET8876880.1"
                     /translation="MDDATHRGAEDRSVISTRRMEAIVAALLFLFGAVFMYQSYELGF
                     RWAVEGPQSGFFPFYVALIICGGSLAVLIGALRGRVGSDDESFVERGQLKQVMSVLIP
                     AGVYILGIELIGVYVASVIYIALFMRILGKYSWLKSVVISVVICVLFFFMFEVWFQVP
                     LYKGMWNLTGWTGY"
     gene            4853..6382
                     /locus_tag="VFO53_11175"
     CDS             4853..6382
                     /locus_tag="VFO53_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HET8876881.1"
                     /translation="MQDLSLLFHGFAVVLSPFNIGLMMLGIVLGVIIGVLPGLGAANG
                     IAILLPLTFTMPPTSAIIMLSCIYWGALFGGAIASILFNIPGEPWSVATTFDGYPLAQ
                     QGRAGEALTASFTSSFVGAFVAVLMITFLAPIIARFALQFGPPEYFSVYLLTFCSFVG
                     MGKGSPAKILVSMMIGFALSTVGIDIVTGQLRMTFGLTSLLKGFDFLIAVIGLFGIGE
                     ILLSMEEGLAFQGAAARINPKVVLETWKKLPRYWMTFIRSTVIGCWMGITPGGATPAS
                     FMSYGAAKKFSKDGDKFGSGVLEGVVAPETAAHAAGTSALLPMITLGIPGSPTAAVLL
                     GGLLIWGLQPGPLLFAEKPDFVWGLIASMYLGNVAGLIVVLALVPLFAGILRIPFSII
                     APVIVVICGIGAYTVNNSMHDIWFMLGFGVVGYVFKKLNYPLAPLVLALVLGNAAENA
                     FRQSMLMSQGSLGILWSNGLVGSISTLALIMLFWPLISKLLRSVRPSRPDKLAEQQPV
                     D"
     gene            complement(6407..7909)
                     /locus_tag="VFO53_11180"
     CDS             complement(6407..7909)
                     /locus_tag="VFO53_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HET8876882.1"
                     /translation="MNANLYALIEAHFPTGTEQPCLLIPGGPVVHYDELAQASARIAN
                     ALVHAGCRPGDRVAVQTDKHWQVVALYLACLRAGLVYLPLNTAYRRGELEYFFGDATP
                     RMIVCRPETLGMVETIAGDATVLTLDAHGGELADRARDEAPFFATVHSQPDDLAAILY
                     TSGTTGRSKGASLTHRNLASNALALVDAWAFTRGDMLLHALPIYHVHGLFVAVHCALL
                     SGARLLWLPKFDARDVAEHLPTATVMMGVPTFYTRLLAEPAFTRECARNIRLFVSGSA
                     PLLPETFAAFRERTGQAILERYGLTETGMITSNPLVGLRAPGTVGRPLPGIALRIVDE
                     AGDACAPGIVGGVEVRGPNVFAGYWNMPDKTRDEFTVDGWFRTGDVGEWVAGDEAKDY
                     LRLVGRAKDMIISGGLNVYPKEIEEHIDALPGVVESAVIGVPDPDFGEAVTAVVVPKP
                     DHTIDEAAVIGALKAEIATFKVPKRVHVVGELPRNAMGKVQKNVLRARFS"
     gene            8028..9332
                     /locus_tag="VFO53_11185"
     CDS             8028..9332
                     /locus_tag="VFO53_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HET8876883.1"
                     /translation="MAPAERIVSLADAGSVKWLAPAGASPHLARFGIAAREDDGIVVA
                     RARLAGAPVLVVAQDETYLAGSVGERHGAALRATFEAARAERPHAIVLLLASGGVRLH
                     EANAAEIELARALSSLFDVRIEGIPVLAVGVGSVFGGASVLACAADRLALLPGTRFGL
                     SGPKVLESVHGKWQLDADDPHDVDAVFGAAARNAAGLVELVADDADAIRAWVAHAVRE
                     RDDFVVAVAAMHARLGRRIAGEARPAPPFEALPCFDGAAPVDDAGRLWRTDGCWLTTP
                     SPGTTIGPDDVHALDSALLAYVAPYADEQTCVVLIEDSAGHAVSRAAEMKLVSQFLAH
                     HASVLALIRARRARLVGLLVGIGHSAAFFTNALQAPERYALPDSRVVAMEPSAVARVT
                     GIEAAELIENDPLLGQPVRHLVARGGAAIVAEATLTALGIGR"
BASE COUNT         1613 a         3016 c         3190 g         1580 t
ORIGIN      
        1 tacgagaacg tcgcgcaatg cgccggcggc tcggcgtcga cgaccgggtt cgacgacggc
       61 ccgccgctcg tgaccggtgc gcagatcggc gacagcggca ccgggctgca tctggccctc
      121 ggcatcgtct gcgcactcta ccagcgcaag acgaccggac gcggccagaa ggtgaacgcg
      181 gcaatgcagg acggcgtgct gaacctgtgc cgcgtgaagc ttcgcgacca acagcggctc
      241 gatcgcaccg gcgtgctgga agagtatccg cagtatccga acggcacgtt cggcgaggcg
      301 gtgccgcgcg caggcaatgc atcgggcggc ggccagccgg gctggatcct gaaatgcaag
      361 ggctgggaga acgacccgaa cgcgtacatc tatttcatca cgcaggcacc ggtgtggaag
      421 gacatctgca aggtgatcgg acgcgaggaa tggctgaccg acccgcgcta tgcgacaccg
      481 aagggacgcc tgccgcacct ctccgagatc ttcgacgaaa tcgagaagtg gacgatgacg
      541 aagacgaagt tcgaggcgat ggagctcctg aacgagtacg acattccgtg cggtccgatc
      601 ctgtcgatga aggagatcgc cgaggagccc tcgctgcgca gcaccggaac cgtcgtcgag
      661 gtcgaccacc cgaagcgcgg caagtacctg accgtcggca acccgatcaa gctgtccgac
      721 agccccaccg acgtcaagcg ctcgccgctg ctcggcgagc acaccgacga ggtgatgaag
      781 gagctcggct actcaccggc gcagatcgaa gagctgcgtt cacaaagggt catctaggaa
      841 gaaccatggc gcacgacaag acggcagtcc gcagcatcat cgacaaggtc aaggccgagg
      901 ggcgcacgtc gctcacggcg cccgagggca agctcgtctg cgacgcgtac ggcattccgg
      961 tgccgcagga aggcgtggcg aagtcggcga gcgaggccgt gcgcctcgcc tccgcgatgg
     1021 gcttccccgt cgtgctgaag atcgtctcgc ccgagatcct gcacaagacc gaagccggcg
     1081 gcgtgctcgt cggcatgaag gacgccgcgg ccgtcgagca gggctatgcg acgatcatgg
     1141 ccaacgcgaa aaagtacaac ggcaaggcga acctggtcgg cgtgcaggtg cagcagatgc
     1201 tgacgggcgg gcaggaagtg atcatcggcg cggtcagcga tccgtcgttc ggcaagctcg
     1261 tcgcgttcgg cctcggcggt gtgctcgtcg aggtgctgaa ggacgtcacg ttccggcttg
     1321 cgcctgcatc gcacgacgac gcgctcgcga tgctcgacgg catcgcggcc gccgagatgc
     1381 tgaagggcgt gcgcgggtcc gaaccggtca accgcgaggc gctcgcgctg atgatcgagc
     1441 gcgtgtcgca actcgcctcc gactttcccg agatcagcga gctcgacctc aatccggtgt
     1501 tcgcgacgcc gaagggcgcg atcgccgccg acgtgcgcat cgtgatcggc gcgccgatgc
     1561 cggcgaaata ccggccgtcg cagcaggaca tcgtgaaggc gatgaaccgc atcatgaagc
     1621 ccgattcggt ggcggtgatc ggcgcgtcgc ccgaagcggg caagatcggc aactcggtga
     1681 tgaagaacct gatcaacggc ggctaccagg gaaagatcta tccgatccac ccgaaggccg
     1741 acgagatcct gggctacaag gcgtaccgga gcgtcaagga cgtgccggcc gacatcgatg
     1801 tggccgtgtt cgcgattcca gccaagttcg tcgcgcaggc actgaccgaa gtcggcgaga
     1861 agggcatccc cggtgcggtg ctgattccgt ccggcttcgc cgaaaccggc aacgtcgagg
     1921 ggcagcagga aatccaggag atcggccgca agtacaacgt gcgcctgatg gggccgaaca
     1981 tctacggctt ctactacacg tggaagaatc tctgcgccac gttctgcacg ccgtacgacg
     2041 tcaagggtca tacggcgctg tcgtcgcagt cgggcggcat cggcatggcg atcatcggct
     2101 tttcgcgctc ggcgaagatg ggcgtgtcgg ccatcgtggg gctcggcaac aaggccgacc
     2161 tcgacgagga cgatctgctc acgttcttcg agcaggacga caacacgcac atcatcgcga
     2221 tgcactgcga ggacctgaag gacgggcgct cgttcgccga agtcgcgaag cgcgtctcga
     2281 agaagaagcc ggtcgtggtg ctgaaggcgg gccgcacggc gatgggcgcg cgcgccgcca
     2341 gctcgcacac cggcgcgctc gccggcaacg acaagatcta cgatgacgtg ctgcgccagt
     2401 cgggcgtcat ccgcgcgccg ggccttcgtg acatgctcga gtacgcccgc gccattccgc
     2461 tgctcgcgac gcctgcgggc gagaacgtcg tgatcatcac cggcgccggc ggatcgggcg
     2521 tgctcctgtc cgacgcggtc gtcgacaacg gcctgaagct gatggcgatg ccgcaggatc
     2581 tcgacgcggc gttccgcaag ttcattccac cgttcggcgc ggcgggcaat ccggtcgaca
     2641 tcacgggtgg cgagccgccg acgacctaca agaacacgat caagctcggc ctcaccgatc
     2701 cgcgcatcca tgcgatcatc ctcggctact ggcacacgat catcacgccg ccgatggtgt
     2761 tcgcgaatct ggtcgtcgag gtgatcgagg aaatgaaggc gcagggcatc cacaagccgg
     2821 tcgtcgcctc gcttgcgggc gacgttcagg tcgaggaagc ggcggaattc ctgttccagc
     2881 acggcgtgcc cgcatacccg tacacgacgg agatgcccgt ggccgtgctg ggcgcaaaat
     2941 acaagtgggc gcgcggcgcc gggctgctgt gatgcggcgg cggtgacgca atcgaatgca
     3001 tgaggagagc tggagcggtt ggcagtgaag gagagcgcgg cgaagaccgg ggcttgccgg
     3061 cggttgttcg cggaaaagga aatgcatgcc gcaaggaggc ggcatgcatc gtcgatatca
     3121 gaatgagacg cggtcgattt tcaatcttgg aggagtcatg agccacattc gtcgtcgcgc
     3181 cctgggcgtt gccctcggcg tcttcgcact cgccgccgta tcgtcagcgc cggttgcggc
     3241 gtgggagccc acgaagccga ttcaattcat cgtaccggca ggaaccggcg gtggcgccga
     3301 ccagatggcc cgcttcatcc agggcgtcgc gtcgaagcac aagctcctga aggagccgat
     3361 catcgtcgtg aacgagtcgg gcggcgccgg tgcgcagggc tttctcgacg tgaagggcag
     3421 caagggcgat ccgaacacga tcatcatcac cctgtcgaac ctcttcacta caccgatggc
     3481 caccggcgtt ccgttcagct ggaaggacct gacccccgtg gcgatgctcg ccctcgacga
     3541 gttcgtgctg tgggtgaatg ccgacacgcc gtacaagacg gcgaccgaat acatcaacgc
     3601 ggtcaaggcg aagccgggca cgttcaagat gggcggcacc gggtcgaagc aggaagacca
     3661 gatcatcacg gcgcagatcg agcaggacac cggggcgaag ttcatctaca tcccgctcaa
     3721 gggtggcggt gacgtcgccg tccagctcgt cggcggccac atcgactcga gcgtcaacaa
     3781 cccgatcgag gcggtggccc agtggcgcgc cggcaagctg cggccgcttt gcgtgttcga
     3841 cagcaagcgg atggactaca aggaaaaggt gacgcagacg atgtcgtgga acgacatccc
     3901 gacgtgcaag gagtcggggc tgccggtcga atacctgatg ctgcgtggca tcttcatgcc
     3961 ggccggcgtg acgcaggacc aggtcaattt ctacatcgat ctgttcaaga agatccgcgc
     4021 aacgcccgac tggaaggaat tcagcgagaa ggcggcgttc aaccagtcgt tcatgaccgg
     4081 gcaggagttc gtgagctggg tgacacaggc cgagaaccag cacaaggcgc tgatggaaaa
     4141 agcgggcttc atggcgaaga agtaggcacg tccgcgctca ggcgatccgc gcgaacgcga
     4201 cgagacggcc gcccggcagg gcggccgtcg acgcgatgcg gagcgccgtt cggcgcgcac
     4261 acacgcgcgg tacgagatcg ggagttcgtt cgtggatgat gcaacgcatc gaggcgcgga
     4321 agaccgcagc gtgatatcga cgcggcgcat ggaggccatc gtcgcggcgc tgctgttcct
     4381 gttcggcgcc gtcttcatgt atcagagcta cgagctcggt ttccgctggg cggtcgaggg
     4441 cccgcagtcg ggcttcttcc cgttctacgt cgcgctcatc atctgtggcg gcagcctcgc
     4501 cgtgctgatc ggcgcgctgc gcgggcgcgt gggcagcgac gacgaaagct tcgtcgagcg
     4561 cggccagctg aagcaggtga tgtcggtgct gattccggcc ggcgtctata tcctcggaat
     4621 cgagctcatc ggcgtctacg tcgcgtcggt gatctacatc gcgctgttca tgcgcatcct
     4681 cggcaagtat tcgtggctga agagcgtcgt gatcagcgtc gtgatctgcg tgctgttctt
     4741 cttcatgttc gaagtctggt tccaggtgcc gctgtacaag gggatgtgga atctcaccgg
     4801 ctggaccggg tactgatacc gcaggcggat ccatgcaaaa caggagagcc gattgcaaga
     4861 cctgagcctg ctctttcacg gcttcgccgt cgtgctgtcg ccgttcaaca tcggcctcat
     4921 gatgctcggc atcgttctcg gcgtcatcat cggcgtgctg ccgggcctcg gtgccgcgaa
     4981 cggcatcgcg atcctgctgc cgctgacgtt cacgatgccg ccgacgtcgg cgatcatcat
     5041 gctgtcgtgc atctactggg gagcgctgtt cggcggcgcc attgcgtcga tcctgttcaa
     5101 cattccgggt gagccgtggt cggtggcaac caccttcgac ggctatccgc tggcgcagca
     5161 gggtcgtgcg ggcgaggcgc tgaccgcgtc gttcacgtcg tcgttcgtcg gcgcgttcgt
     5221 cgccgtgctg atgatcacgt tcctcgcgcc gatcatcgcg cgcttcgcgc tgcagttcgg
     5281 gccgcccgag tacttctcgg tctacctgct gacattctgc agcttcgtcg gcatgggcaa
     5341 ggggtcgccg gcgaagatcc tcgtgtcgat gatgatcggc ttcgcgctgt ccaccgtggg
     5401 catcgacatc gtcaccgggc agttgcgcat gacattcggg ctcacgagcc ttctcaaggg
     5461 attcgacttc ctgatcgcgg tgatcggcct cttcggcatc ggcgagatcc tgctgtcgat
     5521 ggaggagggt ctcgcgttcc agggcgccgc ggcgcggatc aacccgaagg tcgtgctcga
     5581 aacgtggaag aagctgccgc gctactggat gacgttcatc cgcagcacgg tcatcggctg
     5641 ctggatgggc atcacgcccg gcggcgccac gcccgcatcg ttcatgagct acggcgcggc
     5701 gaagaaattc tcgaaggatg gcgacaagtt cggcagcggc gtgctcgagg gtgtcgtcgc
     5761 accggaaacg gccgcgcacg ccgccggcac cagcgcgctg ttgccgatga tcacgctcgg
     5821 cattcccggt tcgccgacgg cggccgtgct gctcggcggc ctgctcatct ggggcctgca
     5881 gccggggccg ctgcttttcg ccgaaaaacc ggatttcgtc tggggcctca tcgccagcat
     5941 gtacctcggc aacgtggccg gcctcatcgt cgtgctcgca ctcgtgccgc tcttcgccgg
     6001 catcctgcgc attccatttt ccatcatcgc gcccgtcatc gtcgtgatct gcggcatcgg
     6061 cgcgtacacg gtgaacaatt cgatgcacga catctggttc atgctcggat tcggcgtcgt
     6121 cggctacgtc ttcaagaagc tcaactatcc gttggcgccg ctcgtgctgg cgctcgtgct
     6181 cggcaatgcg gccgagaacg cgttccgcca gtcgatgctg atgtcgcagg gaagcctcgg
     6241 catcctgtgg tcgaacgggc tcgtcggcag catcagcacg ctcgcgctca tcatgctgtt
     6301 ctggccgctg atctcgaagc tgctgcgcag cgtgcgtccg tcgcggcccg acaagctggc
     6361 cgagcagcag ccggtggatt aagaaaaccg tgcgcgcagc acgttcctag gaaaaccgcg
     6421 cgcgcagcac gttcttctgc accttgccca tcgcgttgcg cggcaactcg ccgacgacat
     6481 gcacgcgctt cggcaccttg aacgtcgcga tctcggcttt gagcgcgccg atgaccgcgg
     6541 cttcgtcgat cgtgtggtcc ggcttcggca cgacgactgc ggtgaccgcc tcgccgaaat
     6601 cggggtcggg caccccgatc accgccgatt cgacgacgcc aggcagcgcg tcgatgtgct
     6661 cctcgatttc cttcggatag acgttgaggc cgccgctgat gatcatgtcc ttcgctcggc
     6721 cgacgaggcg cagataatcc tttgcttcgt ccccggcgac ccattcgccg acatcgccgg
     6781 tgcgaaacca gccgtccacg gtgaattcgt cgcgcgtctt gtcgggcatg ttccagtagc
     6841 cggcgaacac gttcggtcct cgcacctcga caccgccgac gatacccggc gcgcatgcat
     6901 cgccggcttc gtcgacgatg cgcagcgcga tgcccggcag cggccggcca accgttcccg
     6961 gcgcgcgcaa gccgacgagc ggattcgacg tgatcatgcc ggtctcggtc aacccgtagc
     7021 gctcgaggat ggcctgtccg gtgcgctcgc gaaacgccgc aaacgtctcg ggcagcagcg
     7081 gcgccgagcc tgacacgaag agccggatgt tccgggcgca ttcgcgggtg aacgcgggct
     7141 cggcgagaag ccgcgtgtag aacgtcggca cgcccatcat caccgtggcg gtgggcagat
     7201 gttcggcgac gtcgcgcgca tcgaacttcg gcaaccagag gagccgcgcg ccggacagca
     7261 gcgcgcagtg gaccgcgacg aaaagcccgt gcacatggta gatcggcagc gcatgcagca
     7321 gcatgtcgcc gcgcgtgaac gcccacgcgt cgacgagcgc aagcgcgttc gacgcgaggt
     7381 tgcggtgcgt cagcgacgcc cccttcgagc ggcccgtcgt gcccgacgtg tagagaatgg
     7441 ccgcgaggtc gtccggctgc gaatgcaccg tcgcgaagaa gggcgcttcg tcgcgggcgc
     7501 gatcggcgag ctcgccgccg tgcgcgtcga gcgtgagtac cgtcgcgtct cccgcaatcg
     7561 tctccaccat gccgagcgtt tccgggcggc agacaatcat gcgcggcgtc gcatcgccga
     7621 agaaatattc gagctcgccc cttcgatagg cggtgttgag cggaaggtac acgagcccgg
     7681 cgcgcaggca cgcgagatat agcgcgacga cctgccagtg cttgtcggtc tgcacggcaa
     7741 cgcgatctcc gggccggcag cccgcgtgca cgagcgcatt ggcgatgcgc gccgacgcct
     7801 gcgcgagctc gtcataatgg acgaccggtc cgccgggaat cagcaggcag ggctgctcgg
     7861 tgccggtcgg gaaatgcgct tcgataagcg cgtagaggtt ggcgttcatc ccgtcatgat
     7921 cgtgaagccg ccgcgagacg cgcccggggc attcaaggca ttattccata ccgatgacgc
     7981 gacgatcacc gcccgcgatg cacgcttcga ctcacccact gcgtggcgtg gcgccggctg
     8041 aacgcattgt gtcgcttgcc gacgccggca gcgtcaagtg gttggcgccg gccggcgcaa
     8101 gccctcatct tgcgcggttc ggcatcgcag cgcgcgagga cgacggcatc gtcgtcgcgc
     8161 gcgcgcgcct tgccggcgca cccgtgctcg tcgtggcgca ggacgagact tacctcgccg
     8221 ggagcgtcgg tgaacggcat ggtgcggcgc tgcgtgcaac gttcgaggcg gcgcgtgccg
     8281 agcggccgca tgcgatcgtg ctgctgctcg cttcgggagg cgtgcgcctg cacgaggcga
     8341 acgctgcgga aatcgagctt gcgcgcgcat tgtcgtcgct gttcgacgtg cgcatcgaag
     8401 gcatccccgt gctcgcggtc ggcgttggaa gcgtgttcgg cggcgcttcg gtgctcgcgt
     8461 gcgccgcgga ccggctcgcg ttgctgccgg gcacgcggtt cggcctttcc ggtccgaagg
     8521 tgctcgaatc ggtgcacggc aaatggcagc tcgacgccga cgacccgcac gacgtggacg
     8581 ccgtgttcgg cgcggccgca cgcaatgcgg ccggcttggt cgagctcgtt gccgacgacg
     8641 ccgacgccat ccgtgcgtgg gtcgcacacg cggtgcgcga gcgcgacgac ttcgtggttg
     8701 ccgttgccgc gatgcacgcg cgccttggac gccggattgc cggcgaggcg cggccagcgc
     8761 cgccgttcga ggcgctgccc tgcttcgatg gcgcagcgcc ggtcgacgac gcgggtcggc
     8821 tgtggcgaac cgacgggtgc tggctcacga cgccgagtcc gggcacgacg atcggtcccg
     8881 acgacgttca cgcgctcgat tcggcgctgc ttgcctacgt cgcgccgtac gcggacgagc
     8941 agacgtgcgt cgtcctgatc gaggactcgg caggccatgc ggtgtcgcgc gcggccgaaa
     9001 tgaagctcgt ctcgcagttt ctcgcgcacc acgcgtcggt gctcgccctg atccgtgcgc
     9061 ggcgtgcccg tctcgtggga ttgctcgtcg gtatcggtca cagtgcggcg ttcttcacca
     9121 acgcgctgca ggcgcccgag cgctacgcgc tgcccgattc gcgggtcgtc gcgatggagc
     9181 cttcggcggt tgcgcgcgtc accggcatcg aagccgccga gctgatcgaa aacgatccgc
     9241 tcctcgggca gccggtgcgc catctcgttg cgcgcggcgg cgccgcaatc gtggccgaag
     9301 cgacactcac cgcgctgggc atcgggcgat gagtcagtcg cgatcgtcgg tttcacccga
     9361 cgtcgcacgc gcgaggcgat cacggatcgc aacccatgc
//
LOCUS       DASSVX010000261         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_361599, whole genome shotgun sequence.
ACCESSION   DASSVX010000261 DASSVX010000000
VERSION     DASSVX010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361599"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFO53_11190"
     CDS             complement(<1..687)
                     /locus_tag="VFO53_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HET8876884.1"
                     /translation="MNTPDLPKPSLLAAIARKVMLQRGLEPDFSKAVQSELAAIDGPA
                     TKADADIRDLRELPWCSIDNDDSRDLDQLSVYRANGSEANILVAIADVDALVTSGSAI
                     DAHAAINTTSVYTAAGIFPMLPEKLSTDLTSLGEGDERLAIVVDMKVTDAGEVTASTV
                     YRAVVRNHAKLAYNAVAAWLDGAGPAPQCLASVAGLDAQLRAQDAVAQCLKRSRSEKG
                     ALTLETLEVRP"
     gene            843..2117
                     /locus_tag="VFO53_11195"
     CDS             843..2117
                     /locus_tag="VFO53_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019566604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876885.1"
                     /translation="MRVRHTPRYLACIATALFAFATAACAGEIRVACYSDGNECEVTQ
                     DLAKRFESQNADVKVIVDKVPYKAILEQLPVQLAAGEGPDIARVTDLGGLSKYYLDIT
                     PYVKDPKYWEANFGETLKWLRPTPSDKGIYGMMTQLTVTGPFVNKTLFEQAKIPLPGA
                     TATWDQWADATRKVAKATQTQYAIAFDRSGHRFAGPAISMGAKIFDAKGNLVIDDGYK
                     AMAKKFYDWNRDGTTPKEVWGGVGGNTYRDAFEEFANGRVVMYLSGSWQVHRMDQQIG
                     RNFDWIAVPNPCGPAACTGIPGGAAFVALKRTKSPKDVGRFLDFLASEPVYAEYMART
                     ENIPAHAGVAKQGVDYKVSPAAKAALEVFVADVPKLSPIAYAIQGYKYNRALFNPTAA
                     RLGQAIVGEMSLDDALKRISADIDDQVKAAQK"
BASE COUNT          399 a          739 c          742 g          384 t
ORIGIN      
        1 cggccgcacc tcgagcgttt cgagcgtgag cgcgcctttt tcggatcggg atcgcttgag
       61 acactgcgcg acggcatcct gcgcgcgcag ctgcgcgtcg agaccggcaa cgctcgccag
      121 acattgcggt gcaggccccg cgccatcgag ccatgccgcg acggcgttat aggcgagctt
      181 cgcgtggttg cgcaccacgg cgcggtacac ggtgctcgcg gttacctcgc ccgcatcggt
      241 caccttcatg tcgacgacga tcgcgagtcg ctcgtcgccc tcgcccagcg aggtgaggtc
      301 ggtcgacagc ttctccggca gcatcgggaa gatgccggcg gcggtgtaga ccgaggtggt
      361 gttgatcgcc gcgtgtgcgt cgatcgccga gccgctcgtg accagcgcat ccacgtccgc
      421 aatggcaacg agaatgttcg cctcgcttcc gttcgcgcgg taaacgctga gctggtcgag
      481 atcgcgcgaa tcgtcgttgt cgatcgagca ccacggaagc tcgcgcagat cgcgaatatc
      541 ggcgtccgct ttcgtcgccg ggccgtcgat ggccgccagc tcggactgca ccgctttgga
      601 aaagtcgggt tccagtcccc gttgcagcat cacctttcgg gcaatggcgg cgaggagcga
      661 gggtttgggc aggtcgggtg tgttcatcgg tcgcatgacc tgctttttcg tatgcggcgg
      721 tggcggtgcc ggcaaggttg catacagcga ttttctcatg acagcgccgg agcccgcggc
      781 tactccgctg cagctcgtgc cccgctatac aatgcagcca acctcttccc cggagattcg
      841 ccatgcgcgt ccgccacact ccacggtatc tcgcctgcat cgcgaccgcc cttttcgcct
      901 tcgcaacggc tgcgtgcgcc ggcgaaatcc gcgtggcgtg ctattcggac ggcaacgagt
      961 gcgaagtcac gcaggacctc gcgaagcgct tcgagtcgca gaacgccgac gtcaaggtca
     1021 tcgtcgacaa ggttccgtac aaggcgatcc tcgaacagct tccggtccag ctagccgccg
     1081 gcgagggtcc ggatatcgcg cgcgtcaccg atctcggcgg actgtcgaag tactaccttg
     1141 acatcacgcc ctacgtgaag gatccgaagt actgggaagc gaacttcggc gagacgctca
     1201 agtggctgcg gccgacgccg agcgacaagg gcatttacgg catgatgacg cagctcacgg
     1261 tcaccgggcc cttcgtcaac aagacactgt tcgagcaggc gaagattcca ctgccgggcg
     1321 ccacggcgac gtgggaccag tgggcggacg ccacgcgcaa ggtcgcgaag gcaacgcaga
     1381 cgcaatatgc gatcgccttc gatcgcagcg gtcatcgctt cgccggaccg gcgatcagca
     1441 tgggcgcgaa gatcttcgac gcgaagggca atctcgtgat cgacgacggc tacaaggcga
     1501 tggcgaagaa gttctacgac tggaaccgcg acggcaccac gccgaaggag gtatggggcg
     1561 gcgtcggcgg caacacctac cgcgatgcgt tcgaggaatt cgcgaacggc cgcgtggtga
     1621 tgtacctgtc gggcagctgg caggtgcaca ggatggacca gcagatcggg cgcaacttcg
     1681 actggattgc ggttcccaac ccctgcgggc ccgcagcctg cacgggcatt cccggcggcg
     1741 cggcgttcgt ggcgctgaag cgcacgaaaa gcccgaaaga cgtgggtcgc ttcctcgact
     1801 tcctcgcgag cgagcccgtt tatgccgaat atatggcgcg caccgagaac attccggcgc
     1861 acgctggcgt cgcgaagcaa ggcgtcgatt acaaggtctc gcccgcggca aaggcggcgc
     1921 tcgaagtgtt cgtcgccgac gtgcccaagc tctcgccgat cgcctatgcg atccaggggt
     1981 acaagtacaa ccgcgcgctg ttcaatccca ccgccgcgcg cctcgggcag gcaatcgtcg
     2041 gcgagatgtc tctcgacgac gcgctgaagc gcatctcggc ggacatcgac gaccaggtga
     2101 aggcggcaca gaagtagcgc gcccgcgcgc cgccggctgc gcgctcattc cgtggatcaa
     2161 cgcctcgcat cttcgcgcag gttgacatcc gtcccggcgc gcatcgccgg cgtgctgctc
     2221 gacgtcgtcg acgcgccgat gcgggcgctg cagcgcctcg tcgg
//
LOCUS       DASSVX010000262        18297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_528181, whole genome shotgun sequence.
ACCESSION   DASSVX010000262 DASSVX010000000
VERSION     DASSVX010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 18297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18297
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528181"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFO53_11200"
     CDS             complement(<1..377)
                     /locus_tag="VFO53_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HET8876886.1"
                     /translation="MVSPRHALRALLVAAVALAAPLAFAAGTFVNVLTGGTSGVYYPL
                     GVALAGVIGKAMPNVKASVQATKASVENLNLLQAGRGEIAFTLGDSLSDAWKGNEEAG
                     FKTPLKKLRGVAAIYPNYIQIVAR"
     gene            complement(461..1711)
                     /gene="ilvA"
                     /locus_tag="VFO53_11205"
     CDS             complement(461..1711)
                     /gene="ilvA"
                     /locus_tag="VFO53_11205"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HET8876887.1"
                     /translation="MLTLADIEAARERIRGGIYESPCVESIPLSQLTGAHIYCKLDYL
                     QRTGSFKERGARNALLQLSPEQRARGVIAASAGNHALGVAYHSALLGIPVTVVMPKFA
                     PLIKVSNCRHLGATVVLHGTDLSQARVEAEAIAARERLTFIHPFDNEHVMAGQGTIAL
                     EILEQTPDVQAIVAPVGGGGLLAGIGTVVEARKPDVRVVGVEPVHAACLTAALAAGHP
                     VPVTLASTLADGLAVAQAGAFAFEVLRRVVDQVVTVDEATIALAVLRLIELEKSVVEG
                     AGAAPLAAFLAGKLDALKGRNVVLVLCGGNIDLTILDRVIEIGLVADGRLSRFTVSIS
                     DRPGGLARLAEVIASTGASIKEIVHDRAFSGPDLSEVRVVCVVETTGHDHVAALHRAL
                     AAARVAIVGATPDAAASALAAPAL"
     gene            complement(1731..3101)
                     /locus_tag="VFO53_11210"
     CDS             complement(1731..3101)
                     /locus_tag="VFO53_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET8876888.1"
                     /translation="MLPAEIFKAYDIRGIVDRTLTTDGVRLIGQALGSLARERGRDTI
                     VVGRDGRLSGPALLAALSEGIRAAGVNVIDVGMVATPMTYFAAQHLGTQCSVMVTGSH
                     NPPDYNGLKMVIDATTLSGGDIQALRVRIERGEFARGSGSTQSQDVAAAYFARIASDI
                     HLARPVRIAVDCGNGVAGAFAPQLYRRLGCEVVELFCDVDGRFPNHHPDPSQPKNLED
                     LQRRLRDGDCELGLAFDGDGDRLGVVTPAGHVIYADRQLMLFAADVLTRVPGATIIYD
                     VKSTRNLKPWITQHHGVPMMWKTGHSLIKSKMKEVGAALAGEMSGHTFFAERWYGFDD
                     GLYAGARLLEILARHADPSAVLDALPDAMSTPELQIACAEGEQHALIARLREEADFPG
                     ALDVIRIDGLRVEYPDGFGLARASNTTPVVVLRFEADNVQALARIQNAFRRVLTAVKP
                     GVVLPF"
     gene            complement(3123..4178)
                     /locus_tag="VFO53_11215"
     CDS             complement(3123..4178)
                     /locus_tag="VFO53_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8876889.1"
                     /translation="MFAAKRRTSRHARAERAAAGAIVGYRAPRWLAGGHAQTIWPFRL
                     PRASVALRRECVDTPDGDFWDFDWLDADAPPDAPLVVLFHGLEGGSRSHYARALLALL
                     GARGWHGVVPHFRGCGGKPNRLPRAYHSGDHAEVGAMLASIRSRVPAATTLYAVGVSL
                     GGSALLNWLGRAGDDATRTITAAAAVCAPIDLVAAGMAIDRGVNRIYAKHFLTTLRPK
                     SLAIAARFPGLLDASRIRRVRTMGEFDDAVTAPLHGFAGTQDYWTRASSKPWLRSIGA
                     PTLVLNARNDPFIPASSLPAASDVSPAVMLEQPHAGGHAGFASGPFPGNVDWLPGRLL
                     HYFATIGGEPRGRSLSR"
     gene            complement(4188..5312)
                     /locus_tag="VFO53_11220"
     CDS             complement(4188..5312)
                     /locus_tag="VFO53_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET8876890.1"
                     /translation="MILVLEPNIHPESVDYRMLTGQLERLSNVSFRVHREVGTEVTLT
                     EIYLIGNTAALTVEQMQVLPCVAKVVRVSEPYRVLGRHSQGDDRASSFEYNGVHFGQD
                     SLHVFAGLCAVDSRESVAAMMKALRDNGQVCTRMGAYKPRTSPYAFQGFGAECLPYVF
                     ELAGKYGIRVISMEVTHESHVDEIRSALEATGNATGVMLQIGTRNTQNFELLKLVGRQ
                     QQFPVLIKRGFGITLDESLNAAEYLATEGNRKIVFCLRGMKTNMGDPHRNLVDFAHVP
                     VVKRLTRMPVAVDPSHSVGTRARAPDGLLDIFHSVAQGVIAGANMVLVDFHPEPARAL
                     VDGAQALLLSELPHFLADVAIARDACEKRARLAREHAGAG"
     gene            5545..5826
                     /locus_tag="VFO53_11225"
     CDS             5545..5826
                     /locus_tag="VFO53_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HET8876891.1"
                     /translation="MNKLLPLIVLSLVATFAMPANAADNKQNTKMSACQAQAGEKKLE
                     GKDRQNFVNQCLKAKPAKAQSKMAECNAKTKGMSKDDAAKARSECMKAT"
     gene            complement(5964..7007)
                     /locus_tag="VFO53_11230"
     CDS             complement(5964..7007)
                     /locus_tag="VFO53_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HET8876892.1"
                     /translation="MNPLDSWAEEQRSAYLYRACADAENGTIRAELFRRLAGEAEAQA
                     AIWRAQLTARGYPAPGPFIPDARTRLVANLVQWLGPRRLRNVLAAMKVRGMAVYGGAA
                     AAEAVHPMQAAGVSSGLRHPGPAVGGNLRAAVFGVNDGLVSNAGLILGVAGATNDVRM
                     IVLSGVAGMAAGAFAMAAGEYVSVKSQREVHEYQVALERDELTQYPEAETQELALTST
                     AKGVPVRQADRLAKRLVANHENALDTLVRGEPGANPRQLGSPIGAAARACFAFAAGAL
                     VPLAPYLLRVGPDALGWTIGLAAAGFFTVGAALSLFTGRNGVYSGVRMLVLGGAAGAV
                     TFTIGHLIGGGAG"
     gene            complement(7110..8072)
                     /locus_tag="VFO53_11235"
     CDS             complement(7110..8072)
                     /locus_tag="VFO53_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8876893.1"
                     /translation="MNYRRLGRSGLKVSELSFGSWVTYGNQLDENVARECMAAAHDAG
                     VNFFDNAEVYAKGRAESIMGDAFAKLGWRRASYIVSTKFYWGLHDGPNERNTLNRKYL
                     MQAIDGSLARLRLDYVDIVFCHRPDPDTPIDETVFAMHDMIASGKALYWGTSEWSAAE
                     IVMAHAIAERHHLRKPVTEQPQYNLLHRERVETEYARLYDDVGLGTTTWSPLASGLLS
                     GKYNAGVPVDSRASIKGFEWLAERILEPTRLAKVKSLVPIAQELGCTLAQMSIAWCLV
                     NRHVSTVITGASRPAQVVENMKAQDVVARLTPDVLARIDAVTAG"
     gene            8223..10301
                     /locus_tag="VFO53_11240"
     CDS             8223..10301
                     /locus_tag="VFO53_11240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET8876894.1"
                     /translation="MPTSRASSSAAKPPFDADALWAVKRIGTPTLSPDGRLACAPVTS
                     YSIDRNAGTTELWLYPTGFGSRVGLPAKPRKLTAGDKDSDPRFSPDGTNVAFTAKRGD
                     DDEAQIYLIAPDGGEAVRLTRLATGVGAIKWFPDGTRIAFVSWVWPDLRGEAAQAKRY
                     REKKDEKVQAHLTERGEYRYWDHWLTDGREPHVFACDVATGRCEDLLAGTGLALQPWD
                     PAAEHYDIAPDGLELAVTIDPAPEPGMMHRCDVVVVDLRTRRTKNLTSASGMSDEHPC
                     YSPDGRSIAYHAYDTARAFNDQGHLRVLSRKTGASTPVAPAFDRATTHLAWTPDSAAL
                     LSLTEDRGRVGIWRHPLGGSASSLLVPGGVVSGYAQSADGSVLVYTRDTARHPPALFA
                     ASNDGRGERAIESHNRALLARHALGDVREFEVAGFRGEPVQLFVTYPPGFDPKRKWPL
                     MHSIHGGPHAAHHDGWHFRWNTQVFAGQGYVVAAVNYHGSSGFGQAFLETITGRYGEK
                     EFADTEAATDLLVAQGYVDATRLVATGGSYGGFMVAYMNGHTDRYRAFVCHAGCYDWV
                     SMMATDGYRFFAKELGAFHWEDEVRVMRQSPHHYVKQAKTPTLVIHGELDYRVPATQG
                     LQYFNSLKAKDVPARLVYFPDENHWIQKPRNSLLWYREFFAWLGRFAPAKRPSRSAAD
                     KRVRQKAPAA"
     gene            complement(10314..10523)
                     /locus_tag="VFO53_11245"
     CDS             complement(10314..10523)
                     /locus_tag="VFO53_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HET8876895.1"
                     /translation="MEMKRMNGGSLRAAGYDERARKLVVELTSGTLEYSGVPGEVWRR
                     FSTSSSPWSYYRDNIEEEYTAHRTR"
     gene            10623..12416
                     /locus_tag="VFO53_11250"
     CDS             10623..12416
                     /locus_tag="VFO53_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876896.1"
                     /translation="MADRLVVFCALLALVTLSPPAQAAGGAKTLRVAMSIAETSFDPA
                     FASDAASDGIIANVFDTMLDYDYLARPVTLVPRALEAMPQVQDGGRTYVCKVRKGLYF
                     TPDPAFKGQQRELTAGDFAYGLKRVLDPAVKSPWLWLLEGKLVGGSEAREQAAKRGRF
                     DYDASIAGLEVVDRYTLRIRLVAPDLRFPYVLAVPNLAAQAREVVEAYGNDIGAHPVG
                     TGPYMLGEYSRSTRIVLVANPHFRETYYEPAGPIPESSRSAAAALRGKRLPLASRIEI
                     SIIEEGQARWLAFLNREIDFLDILPSEFTAQALDANGRLLPSLAARGIVHDVLLRPNS
                     WWVYFNMEDPVVGGYSLDRIALRRAIGMGYDNDEAIRVLLKGRAVPANGPIPPDIAGY
                     DPTLRTDAQLYDPPLARALLDRFGYKDRDGDGYRETPDGKRLTIERWSTPNSASRESD
                     ELWKKNMDAIGIRIVVKKDRVPELRKMARQGKIPMRSDGWNADYPDAENFMQLLYGPS
                     GPENNARFDLPEFNRLFDRARTLPDSAERTRLFDRMTELVIAYAPWRMTYHVLEDSLR
                     HPWIGTYVPHPIRSQWWQYIDIDPARRASAH"
     gene            complement(12417..12755)
                     /locus_tag="VFO53_11255"
     CDS             complement(12417..12755)
                     /locus_tag="VFO53_11255"
                     /inference="COORDINATES: protein motif:HMM:NF021390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HET8876897.1"
                     /translation="MSQRTLLASIVAIGVLAGCQNGPTKEELEAAKNTIDCERPGERI
                     VIRFADGEARLLMPDATRVVLYQVPSGSGFRYINGLMELRGKGMDVELTREQQAVHLS
                     CKAYEIPKKP"
     gene            complement(12810..14573)
                     /gene="aceK"
                     /locus_tag="VFO53_11260"
     CDS             complement(12810..14573)
                     /gene="aceK"
                     /locus_tag="VFO53_11260"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HET8876898.1"
                     /translation="MDDAAVASPEVIARVLVDEFDRHYALFRDCARAAKRHFQSGNWL
                     AIGHVARDRIDFYDRRVAETVERIEREFRFGGDDGFWAKVRQRFVTLLAEHRQPECAE
                     TFFNTVSTKVLHRTYFHNRCLFVRPAASTEYLDDGAPSYRCYYPRRLGLRATLIDVVL
                     DFGLDLRFADFRADLIHVLAALRRRLPRPFVVDANCQIQLLASLLFRNQTAYAVGRVV
                     NAEKTYPFVAAIKRRGDERLYIDALLTDPLELALLFSANRAYFLVDMEVPSAYVEFLR
                     AMLPDKTASELYTMLGLQKHGKTLFYRDFLHHLRHSTDRLVTAPGIEGLVMTVFTLPS
                     YPYVFKVIRDRIAPSKDTDASRVKQKYALVKHHDRAGRMTDTLEYSDVAIPRERFTGE
                     LLAELETTIATQLENDGAYVIIRHVYIERRLTPLNLFLAGTDAADRARVIGDYGNSLK
                     ELAAVNIFPGDLLFKNFGVTRYGRVVFYDYDEIEYLTDCRFRAIPERPAGVDELSDEV
                     WYPVGTHDVFPEEFATFLLTDASIRHAFMAFHADLLDPRWWQSMQEANRGGRQTEVLS
                     YAEGVRFRSAAATPPSRTILQ"
     gene            complement(14643..15008)
                     /locus_tag="VFO53_11265"
     CDS             complement(14643..15008)
                     /locus_tag="VFO53_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876899.1"
                     /translation="MTHRAIRALAVLVFAAVAVSAAAQTASPASPAPPAAPAAAAAAT
                     PKPECGKPGDYPGNLASDNQKRSWQKDFVAYVDCLKKFIEEQQALAEPHVKASNAAIA
                     EYNAGVKAYNDQIQKAKGD"
     gene            complement(15095..15877)
                     /locus_tag="VFO53_11270"
     CDS             complement(15095..15877)
                     /locus_tag="VFO53_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HET8876900.1"
                     /translation="MAVLKRPPTLAVLAAPEGGWSVARGGPSQLTCPGCGRAMRSLAL
                     PREPLAPLALDVCDDCQALWLDATESQALTPGAVIALFRAIGSSQPTHRDAYPALLPC
                     PRCTTPLSLTQDLQHTTRFTYYRCRYGHGRFTPYVQFLREKNFIRALDPGELARLRAV
                     VTTVRCVSCGAPVPLDKSAVCPYCGAPVMLLDADAVGKALDRLATAESKRTTAQPMSG
                     DAVIAIANVERELAKSRREYESESGVDLIMVGLRALGSLVGH"
     gene            complement(15933..16142)
                     /locus_tag="VFO53_11275"
     CDS             complement(15933..16142)
                     /locus_tag="VFO53_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876901.1"
                     /translation="MKTIMSALSIIAVAGAVAACAPLQLAKSDVDGRVVCDTDRMDRV
                     ERAARRENREVHWINCPLATLRVVS"
     gene            complement(16212..17111)
                     /locus_tag="VFO53_11280"
     CDS             complement(16212..17111)
                     /locus_tag="VFO53_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET8876902.1"
                     /translation="MRWGDFRRSENVEDRTGGAPEGSGGGLPFGGLHIGGGALIILVI
                     VGMLFGINPLEMLGLISGNDGTVQQAPPGNQPGYGPQTGAAPTAADAHKDFAVAVLGD
                     TEDVWGAIFKQMGSRYVPPKLVLYRGVTRSACGRAAAAVGPFYCPGDRDLYLDTSFFN
                     ELATRFGAPGEFAQAYVIAHEVGHHVQNLLGTMDKVQAQMQGGDERTANALSVRLELQ
                     ADCYAGVWGFYAQKRNMIDASDIDSGLRAAAAVGDDRIQKRTQGYVVPDSFTHGSAEQ
                     RTRWFRTGLSSGDLRECNTFAAR"
     gene            complement(17125..18222)
                     /locus_tag="VFO53_11285"
     CDS             complement(17125..18222)
                     /locus_tag="VFO53_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET8876903.1"
                     /translation="MSGTTIGVEHTSEVLADNPLGDPHVRRLAVWLPPGYDGGAVRGR
                     TSARGRRFPVLFDLVGFTGSGLAHINWRPFDENVPERAARLLAERRMGPAIVVFPDCF
                     TSMGGNQYVNSSAIGRYADYLTRELIPFVDREFHTLAAREHRGCFGKSSGGYGAMIHA
                     MRYAQYWGAIADHSGDALFDFCYYTDWPRTLNELDRYRKRPRAAGRIDVTRDERDAAA
                     GIDDGRVRRFLDHVFAKDKLGDAEVHALMNLAMAATYDPDPKVPLGFRLPFNLETGEL
                     IAARWRRWLRNDPVNLVATCKRNLRTMLGIYIDCGWRDQYHLHYGARLLSKRLTQHGI
                     AHVYEEFDDNHSSIDYRMDVSLPFLYRALKP"
BASE COUNT         3040 a         6093 c         6222 g         2942 t
ORIGIN      
        1 cgcgcgacga tctggatgta gttcggatag atcgccgcga caccgcgcaa ctttttcaac
       61 ggggtcttga agccggcttc ctcgttgccc ttccacgcat cggacagcga atcgccgagc
      121 gtgaacgcga tctcgccgcg gcccgcctgc agcaggttga ggttttcgac cgacgccttg
      181 gttgcctgca ccgacgcctt gacgttcggc atggccttgc cgatcacgcc ggcgagtgcg
      241 acgccgagcg ggtaatagac gccgctcgtg ccgccggtca gcacgttgac gaaggttccc
      301 gcggcgaatg cgagcggggc ggcgagggca accgctgcga cgagcagcgc gcgtaatgca
      361 tgacggggtg aaaccatgcg tcctcctggg atgccttgcc tgcgggattg caggctgctt
      421 ttatgggcgg gtcgcgagcg tgcagcgacg ggtcgctcga ttatagtgca ggtgccgcga
      481 gggccgaagc ggccgcgtcg ggcgtcgcgc cgacgattgc aaccctggca gcggccagcg
      541 cacggtgcag cgcggcgaca tgatcgtgcc cggtcgtctc gaccacgcac acgacgcgca
      601 cttccgacag gtcgggtccg gagaacgcgc gatcgtgcac gatctccttg atcgacgcgc
      661 cggtcgatgc aatcacttcg gcgaggcgcg caaggccgcc cgggcgatcg ctgatcgaca
      721 cggtgaaccg cgacaggcgc ccgtcggcga cgagcccgat ctcgatgacg cgatcgagga
      781 tcgtgagatc gatgttgccg ccgcacaaga ccagcaccac gttcctgccc ttgagggcgt
      841 cgagcttgcc cgccaggaac gcggcgagcg gtgccgcacc cgcaccctcg acgacgctct
      901 tctcgagctc gatcaggcgc agcaccgcaa gggcgatcgt cgcctcgtcg acggtgacga
      961 cctgatcgac gacgcggcgc agcacctcga atgcaaatgc gcccgcctgc gcgacggcga
     1021 ggccgtcggc gagcgtcgac gccagcgtga cggggaccgg atgacccgcg gcaagtgcgg
     1081 cggtcaggca tgcggcgtgc accggttcga cgccgacgac gcgaacgtcc ggttttcgcg
     1141 cctcgacgac cgttccgatg cctgcgagca ggccgccgcc gccaaccggc gcaacgatcg
     1201 cctgcacgtc gggcgtctgc tcgaggatct cgagcgcgat cgtgccttgc ccggccatca
     1261 cgtgctcgtt gtcgaacgga tggatgaacg tgaggcgttc gcgtgccgcg atcgcttcgg
     1321 cctcgacgcg cgcctgcgac aggtcggtgc cgtgcaacac gacggtcgca ccgaggtggc
     1381 ggcagttcga caccttgatg agcggcgcga acttcggcat gaccaccgtc accggaatgc
     1441 cgagcagcgc gctgtggtac gcgacgccga gcgcatgatt gcccgccgac gcggcgatca
     1501 cgccgcgcgc gcgttgctcg ggcgaaagct gcagcagcgc gttgcgcgcg ccacgctcct
     1561 tgaagctgcc ggtacgctgc aggtagtcga gcttgcagta gatgtgcgcg ccggtgagct
     1621 gcgacagcgg gatcgactcg acgcacggcg actcgtagat gccgccgcga atgcgctcgc
     1681 gggcggcttc gatgtcggcg agcgtcagca tgtcgaagac gtggatgcgg tcagaagggc
     1741 aggacgacgc cgggcttgac cgccgtgagc acgcgacgga acgcgttctg gatgcgcgcc
     1801 agcgcctgca cgttgtcggc ctcgaagcgc agcacgacga ccggcgtggt gttcgatgcg
     1861 cgcgcgaggc cgaagccgtc gggatattcg acgcgcaggc cgtcgatgcg gatcacgtcg
     1921 agggcaccgg gaaaatcggc ctcctcgcgc agtctcgcga tcagcgcgtg ctgctcgcct
     1981 tcggcgcagg cgatctgcag ctcgggcgtc gacatcgcat cgggcagtgc gtcgagcacc
     2041 gccgacggat cggcatggcg cgcgaggatt tcgagcagcc gcgcaccggc atagagtcca
     2101 tcgtcgaagc cgtaccagcg ctctgcgaaa aatgtgtgac cgctcatctc gcccgcgagt
     2161 gctgcgccga cctccttcat cttcgacttg atcagcgagt ggccggtttt ccacatcatc
     2221 ggcacgccgt ggtgctgcgt gatccacggc ttcaggttgc gcgtcgactt gacgtcgtag
     2281 atgatggtcg cgcccggcac gcgcgtcagc acgtcggcgg cgaacagcat cagttgccgg
     2341 tcggcataga tcacatggcc cgccggggtc acgacgccga ggcgatcgcc gtccccgtcg
     2401 aacgcgaggc cgagctcgca atcgccgtcg cgcagccgcc gctggagatc ctcgaggttc
     2461 ttcggctgcg acgggtcggg atgatgattc ggaaagcgcc cgtcgacatc gcagaaaagc
     2521 tcgacgacct cgcatccgag ccgccggtag agctgcggcg cgaaggcgcc cgcgacgccg
     2581 ttgccgcaat cgacggcgat gcgcaccggc cgtgcgaggt gaatgtcgct cgcaatgcgt
     2641 gcgaagtacg cggccgcgac gtcctgcgac tgcgtgcttc cgctgccgcg cgcgaattcg
     2701 ccgcgctcga tccgcacgcg cagcgcctgg atgtcgccgc ccgacagcgt cgtcgcgtcg
     2761 atcaccatct tgaggccgtt gtaatcgggc gggttgtggc tcccggtgac catgacgctg
     2821 cactgcgtgc cgagatgctg cgcggcgaaa taggtcatcg gcgttgccac catgccgacg
     2881 tcgatcacgt tcacgcccgc ggcgcggatc ccttcggaca gcgcggcgag cagcgccggg
     2941 cccgacaagc ggccgtcgcg gccgacgacg atcgtgtcgc gcccgcgctc gcgggcaagg
     3001 cttccgagcg cctgcccgat caggcgcacg ccgtcggtgg tgagcgtgcg atcgacgatg
     3061 ccgcggatgt cgtatgcctt gaagatctcg gcgggaagca cggaagcgga ccctcgttgc
     3121 gttcagcggg acaaggatcg gccgcgaggc tcgccgccga tcgtcgcgaa gtagtgtagc
     3181 aaccggccgg gcagccaatc gacattgccg ggaaacgggc cgcttgcaaa gccggcatgg
     3241 cccccggcat gcggctgctc gagcatgacc gcggggctca cgtcactcgc cgccggcagc
     3301 gaggaggcgg ggatgaacgg gtcgttgcgg gcgttcagca cgagcgtggg tgcgccgatt
     3361 gaccgcagcc acggcttcga cgaggcgcgg gtccagtagt cttgcgtccc ggcgaagccg
     3421 tgcagcggtg cggtgaccgc gtcgtcgaac tcccccatcg tgcgcacgcg gcgaatccgc
     3481 gacgcgtcga gcaggcccgg gaagcgggcg gcgatcgcca gcgacttggg ccggagcgtc
     3541 gtcaggaagt gcttcgcata gatgcgattc acgccgcggt cgatcgccat ccccgccgcg
     3601 accaggtcga tcggcgcgca aacggcggca gcggcggtta tcgttcgcgt cgcatcgtcg
     3661 ccggcccgcc cgagccagtt caacagtgca ctgccgccga gcgagacgcc caccgcgtaa
     3721 agcgttgtcg cggcgggcac gcgtgagcgg atggacgcga gcatcgcgcc aacttcggcg
     3781 tggtcgcccg agtgataggc gcgtggcagc cgattgggct tgccgccgca accgcgaaag
     3841 tgaggcacga cgccgtgcca accgcgcgcg ccgagcagcg ccagcagcgc acgcgcgtaa
     3901 tgcgatctcg agccgccctc gagaccgtgg aacagcacga cgagcggcgc gtccggcggc
     3961 gcgtcggcat cgagccagtc gaagtcccag aaatcgccgt cgggcgtgtc gacgcattcg
     4021 cgtcgcagcg ccacgctcgc gcgcggaagg cgaaacggcc agatcgtctg ggcgtggccg
     4081 ccggcgagcc agcgcggcgc tcgatacccg acgatcgccc cggcggccgc gcgctcggca
     4141 cgcgcatgcc gtgacgttcg ccgcttcgct gcgaacaccg cgcgacgtca gcccgcgccg
     4201 gcgtgctcgc gcgcaagccg cgcgcgcttt tcgcaggcat cgcgcgcaat cgcgacgtcg
     4261 gcgaggaaat gcgggagctc ggacagaagc agcgcctgtg cgccgtcgac gagtgcccgc
     4321 gcaggctcgg ggtggaagtc gacgaggacc atgttggcgc cggcgatcac gccctgcgcg
     4381 acggagtgaa aaatgtcgag cagtccgtcg ggcgcgcggg cgcgcgtgcc gaccgagtgc
     4441 gacggatcga cggctaccgg catgcgcgtc aggcgcttga caacgggcac gtgcgcgaag
     4501 tcgacgaggt tgcggtgcgg atcgcccatg ttcgtcttca tgccgcgcag gcagaagacg
     4561 atcttgcgat tgccttcggt ggcaaggtac tcggccgcgt tcagcgactc gtcgagcgtg
     4621 atcccgaacc cgcgcttgat cagcaccggg aactgctgct ggcgaccgac gagcttcagg
     4681 agctcgaagt tctgcgtgtt gcgcgtaccg atctgcagca tgacgccggt ggcattgccg
     4741 gtcgcttcga gggcgctgcg gatttcgtcg acatgcgact cgtgcgtgac ctccatcgag
     4801 atcacgcgga tgccgtactt gcccgcgagc tcgaacacgt acggcaggca ttcggcgccg
     4861 aagccctgga acgcgtaagg cgacgttcgc ggcttgtacg cacccatgcg cgtgcatacc
     4921 tgcccgttgt cgcgcaatgc cttcatcatc gcggcgaccg actcgcgcga gtcgacggcg
     4981 cacagtcccg cgaacacgtg caggctgtcc tgtccgaaat gcacgccgtt gtattcgaac
     5041 gacgacgcgc gatcgtcgcc ctggctgtgg cgcccgagca cgcggtaggg ttcggagacg
     5101 cgcacgacct tcgcgacgca gggcagcacc tgcatctgct cgacggtaag cgccgccgtg
     5161 ttgccgatca gatagatctc ggtcagcgtg acctcggtgc ctacctcgcg gtggacgcga
     5221 aaactcacgt tggacaggcg ctccagctga ccggtaagca tccggtaatc gacgctttcc
     5281 gggtggatgt tcggttcgag gacgaggatc atcgcggctt cttctgctgt ggtttcaagg
     5341 gcttgcaaat attgccgacg gcgttcgagc atgccgcggc gcgaggaaga gcgtcatgtt
     5401 gcactgcaag agggtccgcg cgcaacaagg ggcgcaaagc cgtcagcgaa ttcgttgcct
     5461 cggccgttag ggttgccgta gactggcttc gacgtctacg caggtcccag ggcagttccc
     5521 cgaaacaacc ttctcggagt tcgcatgaac aagctgctac cgctcatcgt gttgtcactt
     5581 gtcgcgacat tcgcgatgcc ggccaacgcc gccgacaaca agcagaacac gaaaatgagc
     5641 gcctgccagg cgcaggccgg cgaaaagaag ctcgaaggca aggatcggca gaatttcgtg
     5701 aaccagtgtc tcaaggccaa gccggccaag gcgcagagca agatggccga gtgcaacgcg
     5761 aagaccaagg gcatgagcaa ggacgacgcg gccaaggccc gcagcgaatg catgaaggcc
     5821 acctagcgat acgttttcac cgcactccgg tcgggcgacc tcctgtccgc ccgcccgaaa
     5881 ctaccagacc gcaacctacg tgtttgcggt ctttttttat cgaatgacgc aagcgctccc
     5941 cgtcgtcggc acggtgcttt gcgtcagccg gctccgccac cgatcagatg cccgatcgtg
     6001 aacgtgacgg cgcctgccgc gccgccgagg acgagcatgc gcacgcccga gtagacgccg
     6061 ttgcgcccgg tgaagagcga tagcgcggcg cccacggtga aaaagcccgc agccgcgaga
     6121 ccgatcgtcc atccgagcgc atcgggtccg acgcgcaaca gatagggcgc gagcggcacg
     6181 agcgcgccgg cggcgaacgc gaagcacgcg cgcgcggccg cgccgatcgg cgagccgagt
     6241 tgtcgtggat ttgcgcccgg ctcgccgcgc accagcgtgt cgagtgcatt ttcgtggttg
     6301 gcgacgagtc gcttcgcgag ccggtcggcc tgcctgaccg gcacgccctt cgccgtagag
     6361 gtgagcgcga gctcctgcgt ctcggcctcg gggtactgcg tgagctcgtc acgctcgagc
     6421 gcgacctggt actcgtgcac ttcgcgctgc gatttgacgg acacgtattc gccagccgcc
     6481 atcgcgaacg cgccggccgc catgccggca acgccggaca ggacgatcat cctcacgtcg
     6541 ttcgtcgcgc cggccacccc gaggatcagc ccggcgttcg agacgagccc gtcgttcacg
     6601 ccgaacacgg ccgcgcgcag gttgccgccg accgcgggtc cggggtgccg gagtccggac
     6661 gatacgcccg cggcttgcat cggatgcacg gcttcggcgg ccgcggcgcc gccatagact
     6721 gccatgccgc ggaccttcat cgcggcgagg acgttgcgca gccgccgcgg cccgagccac
     6781 tgcacgagat tggcgacgag gcgggtgcgt gcatcgggaa tgaacgggcc aggcgccgga
     6841 tacccgcggg cggtcaactg cgctcgccag atggcggcct gcgcctcggc ttcgccggcg
     6901 aggcggcgga aaagctcggc gcggatcgtg ccgttctcgg catcggcaca cgcgcgatag
     6961 aggtacgccg agcgctgctc ctcggcccag gaatcgagcg ggttcacggc gtcaccgccg
     7021 cgcggcggcc ggcgactgcc gcgagttctc gtgtttgcag gaatcattcg agcgcaccgc
     7081 gacacgatga ggaacgcaac ccatttcttt caacccgcgg taacggcatc gatgcgcgcc
     7141 agcacgtcgg gcgtgagtct cgcgacgacg tcctgcgcct tcatgttctc cacgacctgc
     7201 gcaggacggc tcgctccggt gattacggtc gacacgtgtc gattcaccag gcaccacgcg
     7261 atggacatct gggcgagcgt acaaccgagt tcctgcgcga tcgggacgag cgatttgacc
     7321 ttcgcgagcc gcgtcggttc gaggatgcgc tcggcaagcc attcgaatcc cttgatcgac
     7381 gcgcgcgaat cgacgggaac gccggcgttg tacttgcctg acaagagacc ggacgcgagc
     7441 gggctccacg tcgtggttcc gaggccgacg tcgtcgtaga ggcgcgcata ttcggtctcg
     7501 acgcgctcgc ggtgcagaag attgtattgc ggctgttcgg tcacgggctt tcggagatga
     7561 tggcgctcgg cgatcgcgtg cgccatcacg atctccgccg cgctccactc ggacgtgccc
     7621 cagtacagcg ccttccccga tgcgatcatg tcgtgcatcg cgaagaccgt ttcgtcgatc
     7681 ggagtgtcgg gatcggggcg gtgacagaac acgatgtcga cgtagtcgag ccgaagccgc
     7741 gcgagcgagc cgtcgatcgc ctgcatcagg tacttgcgat tcagcgtgtt ccgttcgttg
     7801 gggccgtcgt gcagtcccca gtagaacttc gtggacacga tgtaggacgc gcgccgccag
     7861 ccgagcttcg caaacgcgtc gcccatgatc gattcggcgc gccccttcgc gtaaacctcg
     7921 gcgttgtcga agaagttgac gcccgcgtcg tgcgcggcgg ccatgcactc gcgtgcgacg
     7981 ttttcgtcga gctggttgcc gtaggtcacc cacgagccga acgacagttc gctgaccttc
     8041 agtcccgagc ggccaagacg gcgatagttc atgacgttgc gggcttgccg ccgcggccgg
     8101 cgcgcgttgc gcgtcgccgg cggtgggcgg gattcgagga gacggaaata ctgcgcatcg
     8161 gcacgcgttg cggcgtactg ccactacaat cgcggcttca ttctacccgg agcaattcgc
     8221 caatgcctac gagccgcgca agttcgagcg ccgccaagcc gccgttcgac gccgacgcct
     8281 tgtgggcggt gaagcgcatc ggcacgccaa cgctgtcgcc cgatggccgt ctcgcatgcg
     8341 cgcccgtcac gtcgtattcg atcgatcgca acgccggaac caccgagctg tggctctatc
     8401 cgaccggctt cgggtcgcgc gtcggcctgc ccgcgaagcc gcgcaagctc acggcgggcg
     8461 acaaggacag cgatccgcgc ttctcgccgg acggaacgaa cgttgcattc accgcaaagc
     8521 gtggcgacga cgacgaagcg cagatttacc tgatcgcgcc cgacggcggc gaggccgtgc
     8581 ggctcacgcg cctggcgacg ggtgtgggtg ccatcaagtg gtttcccgac ggcacgcgca
     8641 tcgcgttcgt gtcgtgggta tggcccgatc ttcgcggcga ggcggcgcaa gcgaaacgct
     8701 atcgcgaaaa gaaggatgag aaggtgcagg cgcacctcac cgagcgcggc gaataccgct
     8761 actgggatca ctggctgacc gatggccgcg agccgcacgt gttcgcctgc gacgttgcga
     8821 ccggccgctg cgaggatctg cttgcgggca ccgggctcgc gctgcagcca tgggatccgg
     8881 ccgccgagca ctacgacatc gcacccgacg gcctcgagct cgcagtcacg atcgacccgg
     8941 cgcccgagcc gggcatgatg catcgctgtg acgtcgtcgt cgtcgatttg cgcacgcggc
     9001 gcacgaaaaa cctcacgtcg gcaagcggaa tgtcggacga gcacccgtgc tattcgcccg
     9061 acggccgctc gatcgcctat cacgcgtacg acaccgcgcg cgcattcaac gaccaggggc
     9121 acctgcgcgt gctgtcgcgc aagaccggtg cgagcacgcc cgttgcgccg gcgttcgatc
     9181 gtgcaacgac gcatctcgcc tggacgcccg attcggcggc gctgctgtcg ctgaccgagg
     9241 atcgcggccg cgtcggtatc tggcgccatc cgcttggcgg gagtgcctcg tcactgctcg
     9301 ttccgggcgg cgtggtctcg ggttacgcac agtcagccga cggcagcgtg ctcgtctaca
     9361 cgcgcgacac ggcgcgccat ccaccggcgc tttttgctgc atcgaacgac gggcgcggcg
     9421 agcgcgcaat cgaatcgcac aaccgcgcgc tccttgcccg ccacgcgctt ggcgacgtgc
     9481 gcgaattcga ggtggccggc tttcgcggcg agccggtcca gctgttcgtc acgtatccgc
     9541 ccggattcga tccgaagcgc aagtggccgc tgatgcactc gatccacggc ggtccgcacg
     9601 cggcgcatca cgacggctgg catttccgct ggaacacgca ggtattcgcg gggcagggct
     9661 atgtcgttgc cgcggtcaat taccatggct cgtcggggtt cggacaggcg ttcctcgaaa
     9721 cgatcaccgg gcgctacggc gaaaaggaat tcgccgacac cgaagcagcc accgacctgc
     9781 tcgtcgcgca ggggtacgtc gacgccacgc ggctcgtggc aaccggcggc agctacggcg
     9841 ggttcatggt ggcctacatg aacggacaca ccgaccgtta ccgggcgttc gtgtgccacg
     9901 ccggctgcta cgactgggtc agcatgatgg cgaccgacgg ctatcgcttc ttcgcgaagg
     9961 agctcggcgc attccactgg gaggacgagg tgcgcgtcat gcgccagtcg ccgcaccatt
    10021 acgtgaagca ggcaaaaacg ccgacgctcg tgatccacgg cgagctcgac tatcgcgtcc
    10081 ccgccacgca gggcctgcag tatttcaact cgctcaaggc gaaggacgtt ccggcccggc
    10141 tcgtctattt cccggacgag aaccactgga tccagaagcc gcgcaattcg ctgctgtggt
    10201 accgcgaatt cttcgcgtgg ctcggccgct tcgcaccggc gaagcgcccg tcgcgaagcg
    10261 ccgcggacaa gcgcgtgcgg cagaaggcgc ctgccgcctg acggcgggaa cgatcaacgc
    10321 gtccgatgcg ccgtatattc ttcctcgatg ttgtcgcggt agtacgacca cggcgacgac
    10381 gacgtcgaga agcggcgcca tacttcgccg gggacgccgc tgtactcgag cgtcccggac
    10441 gtgagctcca cgacgagctt gcgggcgcgc tcgtcgtacc ccgcggcgcg caggcttccg
    10501 ccgttcatcc gcttcatctc catggcatct cctcgtcgcc cgcggcggcc accggccgct
    10561 ccccgggcgt gcaggaaccg aatgctacga tcggccgctc gttgctttca agcgtcattc
    10621 gcatggcgga ccggcttgtc gtcttctgcg cgctcctggc actggtcacg ctctcgccgc
    10681 ctgcgcaggc ggcaggtggc gcgaagacgc ttcgcgtcgc gatgtcgatc gccgagacga
    10741 gcttcgatcc tgcatttgcg tcggatgccg cctccgacgg catcatcgcg aacgtgttcg
    10801 acacgatgct cgactacgac tatcttgcgc gtcccgtcac gctcgtgcca cgcgcgctcg
    10861 aggcgatgcc gcaggtgcag gacggcggac gcacctatgt ctgcaaggtg cgcaaggggc
    10921 tctacttcac gcccgaccct gccttcaagg ggcagcagcg cgaactgacg gcgggcgatt
    10981 tcgcctacgg gttgaagcgc gtgctcgacc cggccgtgaa gtcgccatgg ctgtggctgc
    11041 tcgagggcaa gctcgtcggc ggcagcgagg cgcgcgagca ggcggcgaag cgcgggcgct
    11101 tcgactacga cgcgtcgatc gcgggactcg aggtcgtcga ccggtacacg ctgcgcatcc
    11161 ggctcgtcgc acccgacctc cgcttccctt acgtgctggc ggtgccgaac cttgcggcgc
    11221 aggcgcgcga agtcgtcgag gcttacggca acgacatcgg cgcgcatccc gtgggcaccg
    11281 ggccctacat gctcggtgag tacagccgca gtacgcgcat cgtcctcgtg gcaaatccgc
    11341 attttcggga aacgtattac gagccggccg ggccgattcc cgaatcgtcg cgcagcgccg
    11401 ccgcggcatt gcgcggcaag cggctgccgc tggcctcacg catcgagatc agcatcatcg
    11461 aggaaggcca ggcgcgctgg cttgccttcc tcaatcgcga gatcgacttc ctcgatatcc
    11521 tgccgtccga gttcacggcg caggcgctcg acgcgaacgg caggctgctg ccttcgctcg
    11581 ccgcgcgcgg tatcgtgcac gacgtcctgc tgcggcccaa ttcctggtgg gtctacttca
    11641 acatggagga cccggtcgtc ggcggatatt cgctggaccg gatcgcgctg cgccgcgcga
    11701 tcggcatggg ctacgacaac gacgaagcga ttcgcgtgct gctcaagggc cgcgcagtgc
    11761 cggcgaacgg cccgattcca ccggacatcg ccggctacga tccgacgctg cgcaccgacg
    11821 cgcagctcta cgatccgccg ctggcgcgcg cactcctcga ccgcttcggc tacaaggacc
    11881 gcgacggcga tggataccgc gagacgccgg acggcaagcg cctcacgatc gagcgctggt
    11941 cgacaccgaa ctcggcgtcg cgcgagagcg acgagctgtg gaagaagaac atggacgcga
    12001 tcggcatccg gatcgtcgtg aagaaggacc gcgtgcccga gctccggaag atggcgcgtc
    12061 agggaaagat tccgatgcgc tcggatggct ggaacgccga ctaccccgat gccgagaact
    12121 tcatgcagct gctgtacgga ccctcgggtc ccgagaacaa cgcacgcttc gacctgccgg
    12181 agttcaaccg gctcttcgat cgcgcacgca cgctacccga ttcggcggaa cgcacgcgcc
    12241 tcttcgaccg gatgaccgag ctcgtgatcg cctatgcgcc ctggcgcatg acctaccacg
    12301 tgctcgaaga cagcctgcgc catccgtgga tcggcacgta cgtgccgcac ccgatccgct
    12361 cgcagtggtg gcaatacatc gacatcgacc cggcgcggcg cgcgtcggcg cactgatcac
    12421 ggctttttcg gaatctcgta ggccttgcag ctcagatgca ccgcctgctg ctcgcgcgtg
    12481 agctcgacgt ccatgccctt gccgcgcagc tccatgaggc cgttgatgta gcggaagccg
    12541 gacccgctcg gcacctggta gagcacgacg cgcgtcgcat cgggcatcag caggcgcgct
    12601 tcgccgtcgg cgaaccggat cacgatccgc tcgccggggc gctcgcagtc gatcgtattc
    12661 ttggcggcct cgagctcttc cttcgtcggg ccgttctggc atccggcgag cacgccgatc
    12721 gcgacgatcg acgcgaggag ggttcgttgc gacatgcagc gctccgtgaa cagcgacgaa
    12781 ggcgagcata acaccggcgc cgcgcgcact cattgcagga tcgtgcgcga tggcggggtc
    12841 gcggccgcgc ttcgaaagcg cacgccctcg gcgtacgaca gcacctcggt ctggcggccg
    12901 ccgcgattgg cctcctgcat cgactgccac catcgcggat cgaggagatc ggcgtggaac
    12961 gccatgaacg catggcggat cgatgcatcg gtgagaagga atgtcgcgaa ctcctccggg
    13021 aagacgtcgt gggtgccgac gggataccac acttcgtccg acaattcgtc gacaccggcc
    13081 gggcgctcgg gaatcgcgcg gaaccggcag tcggtcaggt actcgatctc gtcgtagtcg
    13141 tagaacacga cccgtccgta gcgggtgacg ccgaaattct tgaacagcag gtcgcccggg
    13201 aagatgttga ccgccgcgag ctccttcagt gaattgccgt aatcgccgat cacgcgcgcc
    13261 cggtcggcgg cgtccgtgcc ggcgaggaac aggttgagcg gcgtcaggcg gcgctcgatg
    13321 tagacgtgcc ggatgatgac gtacgcgccg tcgttctcga gctgcgtagc gatcgtcgtc
    13381 tcgagctcgg ccagcaattc gccggtgaag cgttcgcgcg gaatcgccac gtccgagtat
    13441 tcgagcgtat cggtcattcg tccggcgcgg tcatggtgct tgacgagcgc gtatttctgc
    13501 ttgactcgcg acgcatcggt gtccttcgac ggcgcgatgc ggtcgcggat caccttgaat
    13561 acgtacgggt acgacggcag cgtgaacacc gtcatcacga gtccctcgat gcccggcgcg
    13621 gtgacgagcc gatcggtcga atgacgaagg tgatgcagga agtcgcggta gaagagcgtc
    13681 ttgccatgtt tctgcagccc gagcatcgtg tagagctccg atgcggtctt gtccggcagc
    13741 atggcgcgca ggaattcgac gtacgcggag ggcacttcca tgtcgacgag gaagtacgcg
    13801 cgattggccg agaacagcag cgcaagctcc agcggatcgg taaggagggc gtcgatgtag
    13861 agccgctcgt cgccgcgccg cttgatcgcc gcgacgaatg ggtacgtttt ttcggcgttc
    13921 acgaccctgc cgaccgcata cgccgtctgg tttcggaaca gcaggctcgc gagcagctgg
    13981 atctggcagt tcgcatcgac gacgaacggc cgcggcaggc gccggcgcag cgccgcgagc
    14041 acatggatca ggtcggcgcg aaaatccgcg aagcgcaggt cgagcccgaa gtcgagaacg
    14101 acgtcgatca gcgttgcgcg caggccgagc ctgcgcgggt aatagcaccg atacgatggc
    14161 gcaccgtcgt cgagatactc ggtcgacgcg gccgggcgca cgaagaggca gcggttgtgg
    14221 aaataggtgc ggtgcagaac cttggtggag accgtgttga agaacgtctc ggcacactcg
    14281 ggctggcgat gctcggcgag gagcgtgacg aaccgctgtc gcaccttcgc ccagaacccg
    14341 tcgtcgccgc cgaaacgaaa ctcgcgctcg atgcgctcga cggtctccgc gacgcgccgg
    14401 tcgtagaagt cgatgcggtc gcgcgcgacg tgaccgatcg ccaaccagtt gcccgactgg
    14461 aagtggcgct tcgcggccct cgcgcagtcg cgaaacagcg cgtagtgccg gtcgaattcg
    14521 tcgaccagca cgcgcgcaat cacttccggt gatgcgacgg ccgcgtcgtc catggtggcg
    14581 ccgcgttcgc gcgcgatgtc aggcgtcggg ccggacggcg cgccggcgcc cgccgcgcgg
    14641 cgtcagtcgc ccttggcctt ctggatctga tcgttgtagg ccttgacgcc tgcgttgtac
    14701 tcggcaatcg cggcattcga agccttcacg tgcggctcgg ccagcgcctg ctgctcctcg
    14761 atgaacttct tcaggcagtc gacgtaggcg acgaagtcct tctgccagct gcgcttctga
    14821 ttgtcgctgg cgagattccc cggatagtcc ccgggcttgc cgcattcggg cttcggtgtg
    14881 gctgcagcgg ctgcagcggg cgccgcgggt ggcgcgggcg atgcgggcga cgcggtttgc
    14941 gccgcggcgg aaacggcgac ggcggcgaag acgagtaccg cgagggcacg gatggcgcga
    15001 tgagtcacgg aaatctcctc gaaatgatgc tgcgcattct aactccgccg ggcccgatgc
    15061 cctgtgcgcg agcggcacgc cgcgatgcgg aaactcaatg cccgacgaga ctgccgagcg
    15121 cacgcaggcc caccatgatg agatcgacgc cgctttcgct ctcgtattcg cgccggcttt
    15181 ttgcgagctc gcgctcgacg ttggcgatcg cgatcacggc gtcgccggac atcggctgcg
    15241 ccgtcgtgcg cttcgattcc gccgtggcga gccggtcgag cgccttgccg accgcatcgg
    15301 catcgagaag catcaccggc gcgccgcaat agggacacac ggccgacttg tcgagcggaa
    15361 ccggtgcgcc gcagctgacg cagcgcaccg tcgtgacgac tgcgcgcagc cgcgcgagct
    15421 cgcccggatc gagcgcacgg atgaaattct tctcgcgcag gaattgcacg tacggcgtga
    15481 agcgtccatg tccatagcga cagcggtaat aggtgaatcg ggtcgtatgc tgcaggtcct
    15541 gcgtgagcga tagcggcgtc gtgcagcgcg gacagggaag tagggcgggg taggcgtcgc
    15601 gatgcgtcgg ctgcgacgag ccgatcgccc ggaacagcgc gatcaccgcg cccggggtga
    15661 gcgcctggct ttccgtcgcg tcgagccaga gcgcctggca atcgtcgcaa acgtcgagcg
    15721 caagcggcgc cagcggttcg cgcggcagtg caagcgagcg catcgcccgg ccgcagccgg
    15781 gacacgtcag ttgcgaaggg ccgccccgag cgactgacca gcccccttcg ggcgcggcga
    15841 gcacagcgag cgtgggggga cgtttcagca ccgccatcgc gtccgccgat cagcgtgcgc
    15901 agactgcgcg cacccgcacg gtgaggaccg tgttacgaga cgacgcgaag cgtggcgagc
    15961 gggcaattga tccagtgcac ttcccggttt tcgcgccgtg ccgcccgctc gacccgatcc
    16021 atccggtcgg tgtcgcagac gacacggccg tcgacgtcgg acttcgcgag ctgcagcggc
    16081 gcgcaggccg ccacggcgcc cgccacggcg atgatcgaca gcgcactcat gatggttttc
    16141 atgccgcccc tccttcgccg gcaatcatag ggcagcggcg gcggacgccg caagcggcgg
    16201 gccgccggcg gtcagcgggc ggcgaacgtg ttgcattcgc gcaagtcgcc cgacgaaaga
    16261 ccggtgcgaa accagcgcgt tcgctgctcc gccgagccgt gcgtgaacga atcgggaacg
    16321 acatagccct gcgtgcgctt ctggatcctg tcgtcgccga ccgcggccgc cgcacgcagt
    16381 ccgctgtcga tgtcgctggc gtcgatcatg ttgcgcttct gcgcgtagaa gccccatacg
    16441 cccgcgtagc aatcggcctg cagctcgaga cgaaccgaca gcgcgttggc cgtccgctcg
    16501 tcgccaccct gcatctgcgc ctgcaccttg tccatcgttc cgagcaggtt ctgcacgtgg
    16561 tggccgactt cgtgtgcgat cacgtacgcc tgcgcgaatt cgccgggcgc gccgaaacgc
    16621 gtcgcgagct cgttgaagaa cgacgtgtcg aggtacagat cacggtcgcc cgggcagtag
    16681 aacgggccca cggccgccgc ggcgcgcccg catgcggagc gcgtgacgcc acgatagagc
    16741 acgagcttcg gcggcacgta gcgcgagccc atctgcttga agatcgcccc ccacacgtcc
    16801 tcggtgtcgc cgagcaccgc aaccgcgaaa tccttgtgcg cgtcggccgc ggtgggcgcc
    16861 gcgccggttt gcgggccgta accgggctgg ttccccggcg gcgcctgctg cacggtgcca
    16921 tcgttgccgg agatgaggcc gagcatctcg agcgggttga tgccgaacag catcccgacg
    16981 atcacgagga tgatgagcgc gccgccgccg atgtgcaggc cgccgaacgg cagcccgccg
    17041 ccgctgcctt cgggtgcgcc gccggtgcgg tcctcgacgt tctcgctgcg cctgaaatcg
    17101 ccccaacgca tcgccgatct cccttcaggg tttcaatgcg cgataaagaa acggaaggct
    17161 cacgtccatc cggtagtcga tcgacgagtg attgtcgtcg aactcctcgt agacgtgcgc
    17221 gatgccgtgc tgcgtcaggc gcttcgacag caggcgcgcg ccgtaatgca gatgatactg
    17281 gtcacgccac ccgcaatcga tgtagatgcc gagcatcgtc ctgaggttcc gtttgcaggt
    17341 cgcgacaaga ttcaccggat cgttgcgcag ccaccgccgc cagcgcgcgg caatgagctc
    17401 gcccgtctcg agattgaagg gcagccggaa tccgagcggc actttcggat cgggatcgta
    17461 ggtcgctgcc atcgcgagat tcatgagcgc gtggacctcg gcatcgccga gcttgtcctt
    17521 cgcgaagaca tggtcgagga agcgccgcac gcggccatcg tcgatgcctg cggccgcatc
    17581 gcgctcgtcg cgcgtcacgt cgatgcgtcc cgccgcgcgg ggacgcttgc ggtagcggtc
    17641 gagctcgttc agcgtgcgcg gccagtcggt gtaatagcag aagtcgaaca gcgcgtcgcc
    17701 cgaatgatcg gcgatcgcgc cccagtactg tgcgtagcgc atcgcgtgga tcatcgctcc
    17761 atagccgccc gacgacttgc cgaagcagcc gcgatgctcg cgcgccgcga gcgtgtggaa
    17821 ttcgcggtcg acgaacggaa tcagctcgcg cgtcaggtag tcggcatagc ggccgatcgc
    17881 cgacgagttg acgtactggt tgcctcccat cgacgtgaag caatcaggga acacgacgat
    17941 cgcgggtccc atcctgcgct cggcgagaag ccgcgctgcg cgctcaggga cgttctcgtc
    18001 gaacggacgc cagttgatgt gcgcgagccc cgatccggtg aagccgacca ggtcgaacag
    18061 cacgggaaag cgccggccgc gcgcgctcgt gcgtccgcgc acggcgccgc cgtcgtaacc
    18121 gggagggagc cagacggcga gcctgcgcac gtgcggatcg ccgagcggat tgtcggcaag
    18181 cacctccgac gtgtgctcga cgccgatcgt ggtgcctgac acttgttcgg gtcgtttgcg
    18241 gctcatcgtg ggtcgtctcg tctttgcgga tgggcgcgat tgtgacggcc ggcgcac
//
LOCUS       DASSVX010000263         7006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1161336, whole genome shotgun sequence.
ACCESSION   DASSVX010000263 DASSVX010000000
VERSION     DASSVX010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161336"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1054)
                     /locus_tag="VFO53_11290"
     CDS             complement(83..1054)
                     /locus_tag="VFO53_11290"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015094126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET8876904.1"
                     /translation="MSKSALITGITGQDGAYLAKLLLAKGYRVFGVLARRSSDTRWRL
                     AHLGIADDVTLVDGDLVDMTSILRALEVSAADEVYNLGAQSFVATSWRQPLLTAGVTG
                     IGALHVLEAIRIHDPNVRFYQASTSEMFGLIQAEWQDESTPFYPRSPYGVAKLFAHWS
                     TVNYRESFGLHASSGILFNHESPLRGIEFVTRKVTDGVARIKLGKARELRLGNIDAKR
                     DWGFAGDYVEAMWLMLQQERPDDYVIATGVTTTVRDMCRIAFEHVGLDAEKHIVVDPQ
                     FFRPAEVDVLRGNPTKARERLGWSTKTSLHELIAMMVDADMRRVARE"
     gene            complement(1036..1965)
                     /locus_tag="VFO53_11295"
     CDS             complement(1036..1965)
                     /locus_tag="VFO53_11295"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003207046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET8876905.1"
                     /translation="MKRLLVTGQQGFVGTTLSRMVGVERQVAGWSVIDLPGNVDLRDA
                     AATSAMVGNAAPDAVLHLAAQSFVPDAVRDPAATLENNLFGTLNVLQALKKCAFRGRM
                     IYVGTGDVYGVVPEDALPVAETRLPAPRNPYSVSKLAAEALCYQWTATEGMDIVLARP
                     FNHIGWGQSDRFVLSNFARQVVEIRQGRRGPAVAVGDIDVTRDFTDVRDVVHAYFALF
                     ASGASGEVYNVCSGRDRSIRSLLDRLAELAGVAIAIEQDQARLRKAEQRRVCGDPAKI
                     TRTTGWAANTPLDESLAAMLRYWENGEECPNRH"
     gene            complement(2049..3209)
                     /locus_tag="VFO53_11300"
     CDS             complement(2049..3209)
                     /locus_tag="VFO53_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET8876906.1"
                     /translation="MALRVGFNATPLLSPLTGIGQYIVNLGRSLVATGEVDAYSFYGH
                     RWRHEAPAPPPGLRGGDDPQRLLRDIKPFIPFKRELRHAQQQFLFGRGARRFGLDIYH
                     EPNYVPIGYDAPVVVTVHDLSWMRYPQTHPADRVRWLERSMPSALARAAAILVDSDFI
                     RQEVLATFGLDPARVHTVHLGVSSAFRPRSRDETRAALSALDLVHGQYVLAVGTIEPR
                     KNIGHVVAAYARLPERLRRRYPLVIAGASGWRAADLERELHALTARGEVRFLGRVPDE
                     DLPIIYAGAAAFVFASVYEGFGLPPLEAMASAVPVLVADRAALPELTAEAAVKLDPER
                     PDDTSSKLAELLDDAAMQAEFAGRGQRRAAVFTWEACAHRTLRVYRAVEAAA"
     gene            3359..4492
                     /locus_tag="VFO53_11305"
     CDS             3359..4492
                     /locus_tag="VFO53_11305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014002170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8876907.1"
                     /translation="MKVAVVHDWLDTWRGGERVLAQILRIYPDADLFALVDFLSPADR
                     AALDGRRARTSYLQRVPFAKAGFRLLLPLFPRAIESLDVAAYDLVISSSHAVAKGVRT
                     HRRQVHICYCYTPMRYAWDLREQYLSQTGLDRGLRLLAVNALLARLRRWDRAASERVD
                     HFIAISAHIAARIRRCYGRDATVIYPPVTVEHRDDAPKREPLYLTVSQLVPYKRVDLL
                     IEAFRRMPDRELVVVGDGPLRRNLQARAPANVRLLGRLSDVERDGWLARARAFVFAAE
                     EDFGIAPLEAQAFGTPVIAYAGGAACETIAGLDAGAPAGLLFRDQSAHAIVNAVREFE
                     RDAARFTAEACRANARRFGVARFRGEFADFVATHAGVDGNAAR"
     gene            4489..5883
                     /locus_tag="VFO53_11310"
     CDS             4489..5883
                     /locus_tag="VFO53_11310"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HET8876908.1"
                     /translation="MNRSLLRDNAQFFEWAMRLLDPLLVAIVGALAYRVYLDDWVLPE
                     RYALAIFGMSFVCSAFFPFVGLYSPQRGVTLFEEVRRLFNGWLLLATTWFTFLFLSKT
                     GSDFSRAWSIYWITAGFAVHLAARGGIRVTLRTLRRRGYNLRRIAIVGAGALGREIAQ
                     RIRRLPWSGLEIRCFYDDDPALTGTSVIGVTVAGPVDRLLRDLDGGGLDQVWIALPLR
                     AEARIRDLLEGLRRHSVQVRFVPDIFNFTLLHHSMTEIAGLPVINLTESPLEGGNQFL
                     KKLEDVVLSLVILLVASPLMLLIAIGVKLSSPGPVFYRQERVTWNGERFHILKFRTMP
                     IGVEADSGPVWSKHHERRATRFGAFLRRMSLDELPQFFNTLRGEMSVVGPRPERPEFV
                     ERFKQEIPGYMQKHLVKAGITGWAQVNDFRGDTDVQERIQCDLYYIENWSLWFDLRII
                     ALTLVHVVRSRNAH"
     gene            5902..>7006
                     /locus_tag="VFO53_11315"
     CDS             5902..>7006
                     /locus_tag="VFO53_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876909.1"
                     /translation="MTAPASGSPASTTPEVRADASAASSIPGAPRFRREALRIALILA
                     AVLIACVLVYLAAAAPGRWFPRASPKAWSASDLTVVRGSGALVGGQLVITATDANGVA
                     LVNIVTDVTSLDYPGIEWRVAGLAEDADVRLLWRNEFQPDRVNNVPVRVEAGRTVTTV
                     MARNPAWIGHITGLALAIHGTLAQPVVVDGVTAKPMGVIGIARDRLHDWFAFERWNGA
                     SINTIAGGADYQPLPLPLLLALVVGLGGLAVVAMRRLLPGAFTVPLPAIMTAFFLAGW
                     LLLDMRWSFNLMRQERATATRYAGKDAHDKRLANEDGELFAFIEKALAVMPPSPVRVF
                     VASDADYFRGRAAYHLYPHRVYFDPRSNALPPAS"
BASE COUNT         1130 a         2396 c         2250 g         1230 t
ORIGIN      
        1 tcgcgaaatc cccagcattc gcgcagcatc ccggcgcgga acggcgaaac gaccggcatc
       61 tgtcggctcc cgcgtgtcgg cgctactcgc gcgccacccg tcgcatgtct gcgtcgacca
      121 tcattgcgat cagctcgtgc agcgaagtct tcgtcgacca gcccagccgc tcgcgcgctt
      181 tcgtcggatt gccgcgcaat acgtccactt cggcaggacg aaagaactgc ggatcgacga
      241 cgatgtgctt ctcggcgtcg aggccgacat gctcgaacgc gatgcggcac atgtcgcgaa
      301 ccgtggtggt gacgcccgtc gcgatcacgt aatcgtcggg gcgctcctgc tgcagcatca
      361 gccacatcgc ctcgacgtaa tcgccggcga atccccagtc gcgctttgca tcgatgttac
      421 cgagccgcaa ctcgcgcgcc ttgccgagct tgatgcgcgc cacgccgtcg gtcaccttgc
      481 gcgtgacgaa ctcgatgccg cgcaacggcg attcatggtt gaacaggatg ccgctcgacg
      541 catgcaggcc gaagctttcg cgatagttca ccgtcgacca gtgcgcgaac agcttggcga
      601 ccccataggg gctgcgaggg tagaagggcg tcgactcgtc ctgccattcg gcctggatca
      661 acccgaacat ctcgctcgtc gacgcctggt agaaccgcac gttcggatcg tgaatgcgga
      721 tcgcctcgag cacgtgcagc gcaccgatgc cggtgacacc ggccgtcagc aacggctggc
      781 gccacgacgt ggccacgaag ctttgcgcgc cgaggttgta gacctcgtcc gccgcgctga
      841 cttcgagcgc acgcaggatc gacgtcatgt cgacgagatc gccgtcgacg agcgtcacgt
      901 cgtcggcgat gcccagatgc gcgaggcgcc agcgcgtatc ggagctgcgc cgtgccagca
      961 caccgaacac gcggtagccc ttggcgagca gcagcttcgc caggtacgcg ccgtcctgac
     1021 cggtaatgcc ggtgatcagt gccgatttgg acattcttcc ccgttctccc aataacgcag
     1081 catcgcagcg agcgattcgt cgagcggcgt attcgccgcc cacccggtgg tgcgcgtgat
     1141 ttttgccgga tcgccgcaca cgcgccgctg ctcggccttc cgcaatcttg cttgatcctg
     1201 ctcgatcgcg atcgcaacgc cggccagctc cgccagacga tcgaggagcg agcggatgga
     1261 gcggtcgcgg cccgagcaca cgttgtagac ctcgcccgaa gcacccgacg cgaacagcgc
     1321 gaaataggca tgcacgacgt cgcgcacgtc ggtgaaatcg cgcgtcacgt cgatgtctcc
     1381 gaccgcaacc gcgggcccac gccgtccctg cctgatctcg acgacctggc gtgcgaaatt
     1441 cgacaggacg aacctgtcgc tttgtcccca gccgatgtgg ttgaacggtc gtgcaagcac
     1501 gatgtccatg ccttcggttg ccgtccattg atagcaaagc gcctcggccg cgagcttgct
     1561 gaccgaatac ggattgcgcg gcgccggcag acgcgtctcg gccaccggca acgcgtcctc
     1621 gggcaccact ccgtaaacgt ctccggtgcc gacataaatc atccgtccgc ggaacgcgca
     1681 cttcttcaat gcctgaagca cattgagcgt accgaagaga ttgttctcca acgtggcagc
     1741 cggatcgcgc acggcgtcgg gcacgaagct ctgtgccgcg agatgcagca cggcatcggg
     1801 cgccgcgttc ccgaccatcg ccgacgttgc cgccgcatcg cgcagatcga cattgccggg
     1861 caggtcgatc acgctccagc cggcgacttg acgttcgacg ccgaccatgc gcgaaagcgt
     1921 ggtgccgaca aatccctgct gtccggtcac cagcaatcgt ttcacgggtt cgcgtcggcc
     1981 agttcgaagc caccagaaag atcgcaagta tagaggagcg gcgctggttc gccggacagc
     2041 ggcaatgctt acgcggcagc ctccacggcg cgatagacgc gcagcgtccg gtgcgcacac
     2101 gcctcccacg tgaacacggc cgctcgccgc tgaccgcggc ccgcgaattc ggcctgcatg
     2161 gccgcatcgt cgagcagctc ggcgagcttc gacgacgtat cgtcgggcct ctccgggtcc
     2221 agcttcacgg cggcttcggc ggtgagctcg ggcagcgcgg cccggtccgc gacgagaacc
     2281 ggcaccgccg aggccatcgc ctccagcggc ggcagcccga acccctcgta gactgaggca
     2341 aagacgaatg ccgctgcgcc cgcgtagatg atcggcaggt cttcgtccgg cacgcggccg
     2401 agaaagcgga cctcgccccg cgccgtcaac gcgtgcagtt cccgttcgag atcggcggcg
     2461 cgccaaccgc ttgcgccggc aatgacgagc gggtagcgtc tgcgcagccg ctcgggcagt
     2521 cgcgcgtacg ccgccaccac gtgtccgatg ttcttgcgcg gctcgatcgt tccgacggcg
     2581 agcacatact ggccgtggac gagatcgagt gccgaaagcg ctgctcgcgt ctcatcgcgg
     2641 ctgcgcggcc ggaatgcact cgatacgcca aggtgaacgg tatgcacgcg cgccggatcg
     2701 agcccgaacg tcgccagcac ctcctgccgt atgaaatccg aatcgacgag aatggcagcg
     2761 gcccgcgcca acgcggacgg catcgatcgt tcgagccagc ggacgcggtc tgccggatgc
     2821 gtctgcggat aacgcatcca ggaaagatcg tggacggtga cgacgaccgg cgcgtcgtag
     2881 ccgatgggca cgtaattcgg ctcgtgatag atgtcgagtc cgaagcgacg tgcgccgcgg
     2941 ccgaacagga attgctgttg cgcgtgccgc aattcgcgct tgaacgggat gaatggcttg
     3001 atgtcccgca gcaggcgctg aggatcgtcg ccgccccgga gtccgggcgg tggtgccggc
     3061 gcctcgtgcc gccagcggtg tccgtaaaac gaataggcgt cgacctcgcc ggtggccacg
     3121 agtgagcgac ccaggttgac gatgtattgc ccgattccgg tcagcggcga cagcaacggt
     3181 gtcgcgttga agccgacgcg gagcgccatg acgccttcgt ccgatgcatg caaaggcggc
     3241 cgatgttatc actctccctt ccgtgcacca cgttcacggg cgggcgcgta aaattgcgct
     3301 cccaccgctt gttcgccacg atgccgcgaa aacccgcatc cgatccgttg cgctcgtcgt
     3361 gaaggttgcc gtcgtccatg actggctcga cacctggcgc ggcggagaaa gagtgctcgc
     3421 gcaaatcctg cgcatctatc cggatgccga tctgttcgcg ctcgtcgatt tcctgtcgcc
     3481 ggccgaccgc gcggcgctcg acggcaggcg tgcacgaacg tcgtacctgc aacgcgtgcc
     3541 gttcgcaaag gcgggctttc gcctgctgct cccgctgttc ccccgcgcga tcgaatcgct
     3601 cgacgtcgca gcctacgacc tcgtgatatc gagctcgcat gccgtcgcga agggcgtgcg
     3661 cacgcaccgg cgtcaggtgc atatctgcta ctgctacacg ccgatgcgct acgcatggga
     3721 tctgcgcgag cagtacctgt cgcagacggg actcgaccgg ggactgcgcc tgctggcggt
     3781 gaacgcgctg ctcgcgcggc ttcgccgctg ggatcgcgcc gcgagcgagc gcgtcgatca
     3841 tttcatcgcc atctcggcgc acatcgcagc gcgcatccgc cgctgctacg gccgcgacgc
     3901 gacggtcatc tatcctccgg tcaccgtcga gcatcgagac gacgcgccga agcgcgaacc
     3961 gctctatctg accgtctcgc agctcgtgcc ttacaagcgc gtcgacctcc tgatcgaagc
     4021 gttccggcgc atgcccgatc gcgagctcgt cgtcgtcggc gacggtccgc tgcgccgcaa
     4081 cctgcaggcg cgcgcaccgg cgaacgtccg cctgctcggg cggctgtccg acgtcgagcg
     4141 cgacggctgg cttgcgcgtg cccgcgcgtt cgttttcgcc gccgaggagg atttcggcat
     4201 cgcgccgctc gaagcgcagg cattcggcac gccggtgatc gcctacgcgg gcggtgccgc
     4261 gtgcgagacg atcgccgggc tcgatgcggg cgcaccggcg ggtctcttgt tccgcgacca
     4321 gtcggcgcac gccatcgtga acgccgtacg tgaattcgaa cgcgatgccg cgcgcttcac
     4381 cgccgaggcc tgccgagcga atgcgcggcg cttcggcgtc gcgcgattcc gcggcgaatt
     4441 tgcggatttc gtcgcgacgc atgcaggcgt cgacggcaac gctgcccgat gaaccggagt
     4501 ctgctcaggg ataacgcgca gttcttcgag tgggcaatgc gccttctcga tccgctgctc
     4561 gtcgcgatcg tcggcgccct cgcttacagg gtctatctcg acgactgggt gctgcccgag
     4621 cgctatgcgc tggcgatctt cggcatgagc ttcgtatgca gcgccttctt tccgttcgtc
     4681 ggactctatt ccccgcagcg cggcgtcacg ctgttcgagg aggtccgacg tcttttcaac
     4741 ggctggctgc tcctcgcgac gacatggttc acgttcctgt tcctgtccaa gaccggcagc
     4801 gatttttcgc gcgcctggtc gatctactgg attaccgccg gattcgccgt tcacctggcg
     4861 gcgcgcggag gaatccgcgt cacgctgcga acgttgcgca ggcgcggcta caacctgcgc
     4921 cgcatcgcca tcgtcggcgc cggcgcgctc ggccgcgaaa tcgcgcaacg catccgcagg
     4981 ttgccctgga gcgggctcga aatccgctgc ttctacgatg acgatcccgc gctgacagga
     5041 acgtcggtga ttggcgtgac ggtcgccggg ccggtcgatc ggctgctgcg cgatctcgac
     5101 ggcggcgggc tcgaccaggt ctggatcgcg ctgccgctac gcgccgaggc gcgcatccgc
     5161 gacctgctcg aaggtttgcg ccgccattcg gtgcaggttc gcttcgtgcc cgatatcttc
     5221 aacttcacgc tgctgcacca ctcgatgacc gagattgccg gactgccggt gatcaacctg
     5281 accgaatcgc cgctggaagg gggaaaccag ttcctcaaga aactcgagga cgtcgtgctg
     5341 tcgctcgtga tcctgctcgt cgcctcgccg ctgatgctgc tcattgcgat cggggtgaaa
     5401 ctgtcgtcac cgggcccggt cttctatcgg caggaacgcg tgacatggaa cggtgagcgc
     5461 tttcacatcc tcaagttccg gaccatgccg atcggcgtcg aggccgattc gggtcccgtc
     5521 tggtcgaagc accacgagcg ccgcgcgacg cgcttcggcg cgttcctgcg ccgcatgagc
     5581 ctcgacgagc tgccgcagtt cttcaacacc ctccgcggtg aaatgtcggt cgtagggccc
     5641 cgccccgagc ggcccgagtt cgtcgagcgc ttcaaacagg aaataccggg ctacatgcag
     5701 aagcatctgg tgaaggcggg cattaccggc tgggcgcagg tgaacgactt tcgtggcgac
     5761 accgatgtgc aggagcgcat ccagtgcgac ctttactaca tcgagaactg gtcgctgtgg
     5821 ttcgacctgc gcatcatcgc actcacgctg gtgcacgtgg tgcgcagccg gaacgcgcac
     5881 tgaccgacga taatgcttcc gatgacggct cccgccagcg gctcgcccgc gtcgaccacc
     5941 ccggaggtga gggcggatgc aagcgcagcg tcgtcgatcc ccggcgcgcc gcgctttcga
     6001 cgcgaggcgc ttcgcattgc gctgattctc gcggccgttc tgatcgcgtg cgttctcgtc
     6061 tacctcgccg ctgcagcacc tggacgctgg tttccgcgcg cgtcgccgaa agcgtggagc
     6121 gcgagcgatc tcacggtcgt ccgcggttcc ggtgcgctcg tcggcggcca actggtgatc
     6181 acggccaccg atgccaacgg cgtcgcgctc gtcaacatcg tcaccgatgt gacgtcgctc
     6241 gactatccgg gcatcgaatg gcgcgtggcc gggctcgcgg aagatgccga cgtgcgcctt
     6301 ttgtggcgca atgagttcca gcccgatcgg gtcaacaacg tgcccgtccg ggtcgaagcg
     6361 gggcgcacgg tgacgacggt gatggcccgc aatccggcat ggatcggaca tatcacgggg
     6421 cttgcgctcg ccattcacgg cacgctcgca cagccggtcg tcgtcgacgg tgtgacggcc
     6481 aagccgatgg gcgtgattgg catcgcgcgt gaccggctgc acgactggtt cgccttcgag
     6541 cgctggaacg gcgcgtcgat caacacgatc gccggtggcg ccgactacca gccgctgccg
     6601 ctgccgctgc tgctcgcgct cgtcgtcggc ctcggcggtc tcgcggtcgt cgcgatgcgt
     6661 cgattgctgc ccggcgcatt cacggttccg ctccccgcga tcatgacggc gttcttcctc
     6721 gccggctggc tgctgctcga catgcgctgg agcttcaacc tgatgcggca ggagcgcgca
     6781 acggccaccc gttacgccgg caaggacgcg cacgacaagc ggcttgccaa cgaagacggc
     6841 gagcttttcg cgttcatcga gaaggcgctg gcggtgatgc cgccctcgcc cgtgcgcgtc
     6901 ttcgtcgcgt cggacgccga ttatttccgc ggccgcgccg cctatcacct gtacccgcac
     6961 cgcgtgtatt tcgatccgcg cagcaatgcg ctgccgccgg caagcg
//
LOCUS       DASSVX010000264         4802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_378390, whole genome shotgun sequence.
ACCESSION   DASSVX010000264 DASSVX010000000
VERSION     DASSVX010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378390"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..357)
                     /locus_tag="VFO53_11320"
     CDS             complement(58..357)
                     /locus_tag="VFO53_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HET8876910.1"
                     /translation="MELSRESLLDDFSTMLTEAEELLRRAGNETGERAKDLRAQVEAK
                     LLAAKLRLQEMEGEAVDRAKAAARITDDYVHDNPWRAIGAAVAAGFLIGLLVNRR"
     gene            528..1001
                     /locus_tag="VFO53_11325"
     CDS             528..1001
                     /locus_tag="VFO53_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8876911.1"
                     /translation="MRNLRLPALEPGTVAERRGSGYPEPFRSRMGDRVKRRLGDACGL
                     TRFGVNLVTLAPGGQSALRHWHTKEDEFVYVLEGEVVLVTDEGEQVLRAGDCAGYPGG
                     RRNGHHLLNRGGAPARYLEIGNRDPDDAPFYPDDDLAWAEATQGGRYTHKDGRPY"
     gene            1091..1498
                     /locus_tag="VFO53_11330"
     CDS             1091..1498
                     /locus_tag="VFO53_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876912.1"
                     /translation="MADYPAPAPTTSTTESRQPPATGALVAYILFGIAALVGFAGHGF
                     LSLVGIVGVILAYVSRGDSRGTWVESHLTWLIRTFWWSLLWNVLGGIVVLGTLFLGYP
                     IAWVIWVATAIWVIYRVVRGFLLFNDRRPIPSV"
     gene            complement(1500..2951)
                     /gene="dacB"
                     /locus_tag="VFO53_11335"
     CDS             complement(1500..2951)
                     /gene="dacB"
                     /locus_tag="VFO53_11335"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HET8876913.1"
                     /translation="MRRELHRIGLCAIVAVVAIDAHAALPRAVGRAFLDAGIPLNHVS
                     IVVQDTAKTRPLFAYDANRSRSPASVMKLVTTFAALELLGPDYRWKTEAYVDGPLVDG
                     ELHGDLILKGYGDPKITVEQWQSLIADLRTRGLSAIDGDLALDRSLFALPPHDPGAFD
                     HEPLKPYNVGPDALLVNFKSLKLTLVPDASGTGVTASADPPLDVVTLGKPPPVSNDDC
                     AGAPAPVTPSIDDRGDRAAISFTGTYPLRCGERDWWIAMLDHPHYVRAMFETYFRAAG
                     GRFDGSVKERRVPAGAMPFAVLESPPLADIVRDVNKLSNNVMARQIFLTLATARDTPP
                     ATPAKAASVVKRWLAARKLAMPGLVLENGSGLSRHERNSAIGLNRLLLAADASNVREE
                     FERSLAVAAIDGTVERRFRDDAVAGQALLKTGSLEGVRALAGYVLDAAGRRFSVVAIV
                     NDPHARRAVPALDYLVQWVYRNGATWDPAQQRR"
     gene            complement(2941..4266)
                     /locus_tag="VFO53_11340"
     CDS             complement(2941..4266)
                     /locus_tag="VFO53_11340"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HET8876914.1"
                     /translation="MPVSLDTGADWAARSRASVWHPCTQMKQHERTPPVPIARASGAW
                     LYGFDGRRYLDAVGSWWVNLFGHAEPRINAAIAQQLSELEHVMLAGFTHRPVIELSER
                     LAALAPPGLAHAFYGSDGASATEIALKMSFHCWRQRGERGKDRYARLAGGYHGETVGA
                     LSVTDVALFRDAYAPLLRSQPVLPSPGVNDAIDAPAAQRATDEALAALERFLAEHAAT
                     TAALIVEPLVQGARGMAMYAPRYLSAARELTQRHRVHLIADEIMTGFGRTGTMFACEQ
                     AAIVPDFLCLSKGITGGYLPLSCVLATDDVFGTFYDDDVTRGFLHSHSYTGNALACRA
                     ALAVLDIFRDDDVIETNAAKAVRWRRMASPLAAHARVRGFRQCAMIIAFDVDGADAGF
                     ATRFAREALRRELVLRPIGSTVYFMPPYTLTDDEFALLVERTVQVIDAS"
     gene            complement(4257..>4802)
                     /locus_tag="VFO53_11345"
     CDS             complement(4257..>4802)
                     /locus_tag="VFO53_11345"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8876915.1"
                     /translation="VFLASRAEVEWFIDGMQRFFPDAWRDFTHFLPRAERADVLHSYY
                     RRLIDPDPRIHGPAASAWDRYESACSTLLPKPAGAARQEGNGGALAIARIEAHYFVHD
                     SFIAPNQILDRLAAIRHLPCTIVQGRYDVICPPISAGALARAWPEAELIIVPDAGHSV
                     REPGTTRELVAAVQRMQPRCR"
BASE COUNT          740 a         1626 c         1667 g          769 t
ORIGIN      
        1 gctgtaatta ttcgcactaa cggataatta cagctctgac cccgaagtcc ggcgccatca
       61 gcggcggttg acgagcaggc cgatcaggaa gccggcggcg accgcagcgc cgatcgcgcg
      121 ccacggattg tcgtgcacgt agtcgtcggt gatgcgcgcc gccgctttcg cgcggtcgac
      181 cgcctcgcct tccatttcct gcagccgcag cttcgcggcg aggagcttcg cctcgacctg
      241 tgcgcgcaga tccttcgcgc gctcgccggt ttcgttgccc gcacgtcgca gcagttcctc
      301 ggcttcggtc agcatcgtgg aaaaatcgtc gagcagcgat tcgcgggaga gctccatcgt
      361 cgactccttc gggcgggcat tcgggccggc cattatagcg gcgccgggca cggcgcgagg
      421 cggcggccga ttgcgtccgg gtggcgggct gcaagaccgg tcgacgcttc ttgtccgccg
      481 gccgcgaaca tgctttgatt gcgcaaggcg atacacgagg acgttcgatg cgaaacctgc
      541 gcctgccggc actggagccc ggaaccgtgg ccgagcgccg cggctccggc tatcccgaac
      601 cgttccgctc gcggatgggc gatcgcgtga agcgacgcct cggcgatgcg tgcggattga
      661 cgcgcttcgg cgtcaacctc gtgacgctcg cgcccggcgg acagtcggcg cttcggcatt
      721 ggcacacgaa ggaggacgaa ttcgtgtacg tgctcgaggg cgaggtggtg ctcgtcaccg
      781 acgagggcga gcaggtgctt cgcgccggcg attgcgcggg ctacccgggc ggccgccgca
      841 atggtcatca tctgctgaac cgcggcggcg cgccggcacg ctacctcgag atcggcaacc
      901 gcgatcccga cgacgcgccg ttctatccgg acgatgacct tgcatgggcc gaggccacgc
      961 agggcggccg ttacacgcac aaggacggcc ggccgtattg atcgcgccac gaggcccccc
     1021 atcttatccg ccgggcccaa tcgcgctcgc gcgatggttc gttcgcaggt agcgctaacc
     1081 ggagatcccg atggctgatt accccgctcc cgctccaacg acttcgacga ccgagtcgcg
     1141 ccagccgccg gccaccggcg cgctggtcgc ctacattctg ttcggcatcg cggcgctcgt
     1201 cggattcgcc ggccacggtt ttttgagcct cgtcggcatc gtcggcgtga tcctcgctta
     1261 cgtttcgcgt ggcgattcgc gcggaacgtg ggtcgagtcg catctcacgt ggctgattcg
     1321 gacgttctgg tggtcgctgc tctggaacgt gctcggtggc atcgtcgtgc tgggaacgct
     1381 gttcctcggt tacccgatcg cctgggtcat ctgggtggcg acggcaatct gggtgatcta
     1441 ccgcgtggtc cgcggcttcc tgcttttcaa cgaccggcga cccatccctt cggtgtgaat
     1501 cagcggcgct gctgcgccgg gtcccaggtc gcgccgttgc ggtacaccca ctgcacgagg
     1561 taatcgagcg cgggcacggc acgccgggcg tgcgggtcgt tgacgatcgc gacgacgctg
     1621 aagcgccgtc ccgcggcatc gagcacgtag ccggcgagcg cgcgcacgcc ttcgagcgaa
     1681 cccgtcttca acagcgcctg cccggccacg gcatcgtcgc gaaagcgacg ctcgacggtg
     1741 ccgtcgatcg ccgcgaccgc cagcgatcgc tcgaattcct cgcgcacgtt gcttgcgtcc
     1801 gccgcgagca gcagccggtt gagcccgatt gcgctgttgc gctcgtgccg cgacaagccg
     1861 gacccgttct cgagcacgag cccgggcatc gcgagcttgc gcgccgcgag ccagcgcttg
     1921 acgacgctcg ccgccttcgc cggcgtcgcg ggcggcgtgt ccctcgcggt cgcgagcgtc
     1981 aggaagatct ggcgcgccat cacgttgttc gacagcttgt tgacgtcgcg gacgatgtcg
     2041 gccagcggtg gcgactcgag cacggcgaac ggcatggcgc ctgccggcac gcgccgctcc
     2101 ttcacgcttc cgtcgaagcg tccccccgcg gcacgaaaat aggtctcgaa catcgcacgc
     2161 acgtaatgcg gatgatcgag catcgcgatc caccagtcgc gctcgccgca gcgcagcgga
     2221 taggtgccgg tgaagctgat cgccgcgcga tcgccgcgat cgtcgatcga cggcgtgacc
     2281 ggtgccggtg cgccggcgca gtcgtcgttc gacaccggcg gcggcttgcc aagcgtcacg
     2341 acgtcgagcg gcggatcggc gctcgccgtc acgccggtgc cgctcgcgtc cgggacgagc
     2401 gtcagcttca gcgacttgaa attgacgagg agcgcgtcgg gtccgacgtt gtacggtttc
     2461 agcggctcgt ggtcgaaggc gcccggatcg tgcggcggca gcgcgaacag gctgcggtcg
     2521 agcgccaggt cgccgtcgat ggcggacagc cccctggtgc gcaggtcggc gatcaacgac
     2581 tgccattgct cgacggtgat cttcgggtcg ccgtaaccct tgaggatcag gtcgccgtgc
     2641 agttcgccgt cgacgagcgg gccgtcgacg tacgcctcgg tcttccagcg gtagtcgggt
     2701 ccgaggagct cgagtgccgc gaacgtcgtg acgagcttca tcaccgacgc gggactgcgc
     2761 gagcggttcg cgtcgtacgc gaaaagcggt cgcgtcttcg ccgtgtcctg cacgacgatg
     2821 ctcacgtgat tgagtggaat gccggcatcg aggaacgcgc ggcccaccgc acgcgggagc
     2881 gcggcgtgcg cgtcgatggc aaccaccgcg acgatcgcgc acaaaccgat ccgatgcagc
     2941 tcacgacgca tcgatcacct ggacggtgcg ctcgacgagc agtgcgaatt cgtcgtcggt
     3001 cagcgtgtac ggcggcatga aatagacggt gctgccgatc gggcgcagca ccagctcgcg
     3061 gcgcagtgcc tcgcgggcga atcgcgttgc gaaaccggcg tccgcgccgt cgacgtcgaa
     3121 cgcgatgatc attgcgcatt ggcgaaatcc gcgcacgcgt gcatgcgccg ccagcggcga
     3181 cgccatccgg cgccagcgca cggccttcgc cgcgttcgtc tcgatgacgt cgtcgtcgcg
     3241 gaagatgtcg aggacagcga gcgccgcgcg gcacgcgagc gcgttgccgg tgtacgaatg
     3301 cgagtgcagg aagccgcggg tcacgtcgtc gtcgtagaag gtgccgaaca cgtcgtcggt
     3361 cgcgagcacg cacgacagcg gcagatagcc gccggtgatg cccttcgaca ggcacaggaa
     3421 gtcgggcacg atcgcggcct gctcgcaggc gaacatcgtt ccggtgcgcc cgaagccggt
     3481 catgatctcg tcggcgatca ggtgcacgcg atgacgctgc gtgagctcgc gcgcggcgct
     3541 cagatagcgc ggcgcataca tcgccatgcc gcgcgcgccc tgcacgagcg gctcgacgat
     3601 cagcgcggcg gtcgtcgccg catgctcggc gagaaagcgc tcgagcgccg cgagcgcctc
     3661 gtcggtggcg cgctgcgcgg ccggcgcgtc gatcgcatcg ttgacgccgg gcgagggcaa
     3721 tacgggctgc gaacgaagca gcggcgcata cgcgtcgcgg aacagcgcga catccgttac
     3781 cgacaacgcg ccgaccgttt cgccgtgata gccgcccgcg agccgcgcgt agcggtcctt
     3841 gcctcgctcg ccgcgctggc gccagcagtg gaagctcatc ttgagcgcga tctcggtcgc
     3901 cgacgcgcca tcggagccgt agaacgcgtg cgcaagaccc ggcggtgcga gtgccgcaag
     3961 ccgctccgac agctcgatca ccgggcgatg ggtgaagccg gcgagcatca catgctcgag
     4021 ctcggacagt tgttgcgcaa ttgccgcgtt gatgcgcggc tcggcgtggc cgaacaggtt
     4081 cacccaccac gagccgaccg catcgagata gcggcggcca tcgaaaccgt agagccatgc
     4141 gcccgatgcg cgcgctatcg gcaccggcgg cgtccgctcg tgctgcttca tctgcgtgca
     4201 cggatgccag acggacgcgc ggctgcgcgc cgcccaatcg gcgcctgtgt cgaggctcac
     4261 cggcatcgcg gctgcatccg ctgcaccgcg gccacgagct cgcgcgtcgt gccgggctcg
     4321 cgcaccgaat gcccggcatc gggcacgatg atcagctcgg cctcgggcca tgcgcgcgca
     4381 agcgcgccgg ccgatatcgg cggacagatc acgtcgtagc ggccctgcac gatcgtgcag
     4441 ggcagatggc gtatcgccgc aaggcggtcg agaatctggt tcggcgcgat gaacgaatcg
     4501 tgcacgaagt agtgcgcttc gatgcgcgcg atcgcgagtg cgccgccgtt gccttcctgg
     4561 cgcgccgccc ccgccggttt cggaagcagt gtcgagcacg cgctctcgta gcgatcccac
     4621 gccgacgccg ccgggccgtg gatacgcggg tcgggatcga tcaggcgccg gtaatagctg
     4681 tggagaacgt cggcacgctc ggcccgtggc agaaagtgcg tgaaatcgcg ccaggcgtcg
     4741 gggaagaagc gctgcatgcc atcgatgaac cattcgacct cggcgcggct tgcgaggaac
     4801 ac
//
LOCUS       DASSVX010000265         6591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1211622, whole genome shotgun sequence.
ACCESSION   DASSVX010000265 DASSVX010000000
VERSION     DASSVX010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211622"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            551..1066
                     /locus_tag="VFO53_11350"
     CDS             551..1066
                     /locus_tag="VFO53_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876916.1"
                     /translation="MKRLFVLSGFTVALLSGVPCALAQSSPPPLPSLTPQQSATLEQR
                     LDSYRQQTDARASRGEITADEADRLIRWREWQIARQVAGVSSTQRPGARSDVPPDYVE
                     PPPRDYGESVPPDYVEAPSRDYVVVEPPPYYGPYYRYPAPYYWGPRSYYWGPAVCAGG
                     FGRHFGGRICF"
     gene            1126..2613
                     /locus_tag="VFO53_11355"
     CDS             1126..2613
                     /locus_tag="VFO53_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HET8876917.1"
                     /translation="MLACAALVVSTASAAFGFEDIALRAKNLAASAYKKPDVNLPKAL
                     QELTYDQYRDIRFKPDRALWRKAKLPFEVMFFHQGLYYNYPVRMHEVTPDGVHDIRFD
                     PSLFDYGANHLDPKELDGLGFAGFRVHFALNSPRYKDEVLVFQGASYFRALGKGQRYG
                     ISARALAIDTALMSGEEFPRFTEFWIERPAPQARELRIYALLDSPRATGAYRFVLKPG
                     VDTVLDVRGQIYLRQNVSKLGIAPLTSMYYFGENHRLGIDDFRPEVHDSDGLSVQSGT
                     GEYIWRPLVNPKRLLVTSFAVDNPLGFGVLQRDRRFFDYEDLEARYETRPSVWIEPKG
                     NWGNGRIELVQIPTPDETNDNIVAYFVPDASLAPLQPYDFEYRMLWQKENETRPTLAY
                     VAQTRRGHGYLRKPDDSIAFMIDFEGDVLRNLPADTPVQGVVSTDSNVELVEANAYHN
                     DVTNGWRLALRIRRQDDKKPGELRAFLRNGGTTLSETWSYILPTN"
     gene            2586..5165
                     /gene="mdoH"
                     /locus_tag="VFO53_11360"
     CDS             2586..5165
                     /gene="mdoH"
                     /locus_tag="VFO53_11360"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HET8876918.1"
                     /translation="MELHPADQLSAGVAALLRRYLARLPLAAERRAALLADALQCAHD
                     APDALGRLHAALSAAAANSANAAYATIGARVRLATARQGAAADAIQCHDIHGRERLET
                     TPRLARSPMAPHAWFGRRARAEEADRVPSGRDRRSAAKPLDPGGRWRQRAAHRRLVLV
                     TLIVVQTLVATDYMALILPYHGRRPLEIGILVLFGVLFAWISAGFWTALAGFWLLARG
                     HDRYAISRRMIGDGPASIPGNARTALVMPICNEDVPRVFAGLRATFESLRATGALDRF
                     NFFVLSDTGNADTRVAELDAWLALCNAVDGFGRIFYRWRQHRIKRKSGNIADFCRRWG
                     RKYRYMIVLDADSVMSGDCLTALVRIAEANPDAGIIQTAPRAAGRDTLYARVQQFATG
                     VYGPLFTAGLHAWQFGESHYWGHNAIIRVAPFIRYCALGRLPGHGALSGEILSHDFVE
                     AALMRRAGWGVWIAYDLPGSYEEMPPNLIDELSRDRRWCQGNLMNFRLFWMKGLHPAH
                     RAVFMTGVLAYLSAPLWFLFLILSTVLLAIHTLSEPAYFTEPYQLFPIWPEWRPEWAI
                     TLFSATALLLFLPKLLAGLRVAFTDPARHGGRMHLAVSLAGEMLLSALLAPIRMLFHT
                     QFVLTSLAGRTVRWKSPPRGDNETGWRAAFARHGVHTFVGIVWAAIAWWLNPSYLWWL
                     LPVVGALALSIPLSVVTSRAGLGRKLRRRKYFLIPEESEPPDVIRATRRHWHRAAAPA
                     DFAAAVVDPIVNALVCAQAVSRPVTGWPREATADALVERALRQGLDALTPADRGRLLA
                     DPAALSALHFAVWTSPAAHLSWREAVARPVARSAAPRLHAHEESEASGPGAGLAASHP
                     VHG"
     gene            5187..>6591
                     /locus_tag="VFO53_11365"
     CDS             5187..>6591
                     /locus_tag="VFO53_11365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET8876919.1"
                     /translation="MTSPANHRTTALPRAAPMPRRLPGTNAPEAVEPLFDSIWPFLAI
                     VIVLVALAFIDFLLVQPAPSARITLLALRLGSSAALLILAGLTLRRLFAQRARLQQAL
                     RASEERLVSSLSDIQMRKQAEALLAEEKERAQVTLASIADAVVTVDTSGRIEFLNSAA
                     ERVTGWTLAEARQRPVAEVFAVVDEATGAPIPDPVGRALTDGAIVEAEGNVVLLCRGA
                     ESIAIDYSVAPIRDRAGNSVGAVLVIQDMSRERQYAARLSNLASHDALTGLLNRREFE
                     QRVRAIVEQREGPEGQHAVLYLDLDQFKVVNDTSGHAAGDELLRQVGALLRPRLREGD
                     VLARLGGDEFGVLLPHCPPAPALRIAEALRKAIIDFRFAWKNRSFTIGVSIGLVNLAD
                     GPHTLASVLSAADAACYLAKDKGRNRVQVYRPEDSEVTLRRGEMEWVHRLHRALAEDR
                     LCLYSQPMRAMHAPGPHV"
BASE COUNT         1013 a         2288 c         2176 g         1114 t
ORIGIN      
        1 ccgctcgcgt acgacatgcg cgcaacgcgt gtcggaggat cggcgtcggg cccggactcc
       61 tgcggttgcg gttgcgcgca cacggaaagc gacagcatcg cggaaacgat tgcaagcaaa
      121 tgcgtcaggc gtggcaaggc gttcgatggg atcatggcgt gtcctgtaac gctgtgggtc
      181 gggtcctaca aggcatgcga gcgtcctccc gcgcggttgt aattgctact cgccgcacgc
      241 gttcgcccca gccatcgacg gtttccgcga agtgtctcgc aaacatcacg ccaccgaaaa
      301 cgcgcgacgg catgcgtttg atgacaggac tttcgtcgcc ggtggacgac agtggtgacc
      361 cggcaatgtc gcggcgccag cgcgcggtat gatgaccggc gcgaccatat cgcgctttcg
      421 gactcccgac tcgcgagcac ggccggtgcg gcgagcatgt cgaacgacga accgcgtcat
      481 tcgcgcgcgg tcgaatctgc gttccgtaaa gcgctgtctt tgccacagga aaagcaacaa
      541 ggagccaacg atgaaacggc tgttcgtgtt gtcgggattc acggtcgcgc tgctgtcggg
      601 cgtcccgtgc gcgctcgcgc aatcatcgcc accgccgctg ccgtcgctga cgccgcagca
      661 atcggcgacc ctcgagcagc gactcgactc gtatcgccag caaaccgacg cacgcgcgtc
      721 gcgcggggag atcaccgcgg atgaagccga tcgcctgatc aggtggcgcg aatggcagat
      781 tgcccgccag gtggcgggtg tgtcgtccac gcaacgcccg ggtgcacgaa gcgacgtgcc
      841 acccgactac gtcgagccgc caccgcgcga ctacggcgag tccgtgcccc ccgactacgt
      901 cgaggcgcca tcgcgcgatt acgtcgtggt cgagccgccg ccctactacg ggccgtacta
      961 ccgctatccc gcgccgtatt actggggacc gcgttcgtat tactggggac ccgccgtatg
     1021 cgccggcggg ttcggccgcc acttcggcgg ccggatctgc ttctgactcg catccggcag
     1081 tttgccgaca caaggaggcg tgacatcgcg gcgctcgctg ccgtattgct cgcctgcgca
     1141 gcgctcgtcg tctccacggc aagcgctgcg ttcggcttcg aagacattgc gcttcgggcg
     1201 aagaatctcg ccgccagcgc ctacaagaaa cccgacgtca acctgccgaa ggcgctgcag
     1261 gaactgacgt acgaccaata ccgcgatatc cgcttcaagc ccgatcgggc gctgtggcgc
     1321 aaggcaaagc tgccgttcga ggtgatgttc tttcaccagg ggctctatta caactacccg
     1381 gtccgcatgc acgaggtgac gcccgacggc gtgcacgaca tccgcttcga cccgagcctc
     1441 ttcgactacg gcgcgaatca cctggacccg aaggagctcg acggtctcgg cttcgccggg
     1501 tttcgcgtgc acttcgcgct caattcgccg cgctacaagg atgaagtgct ggtcttccag
     1561 ggcgccagct atttccgcgc gctcggcaag ggacaacgct acgggatatc cgcgcgtgcg
     1621 ctcgccatcg atacggcact catgtcgggc gaggaatttc cgcgcttcac cgaattctgg
     1681 atcgagcgcc ccgcaccgca ggcgcgcgag cttcgcatct atgcgttgct cgactcgccg
     1741 cgcgcgaccg gcgcgtatcg cttcgtgctg aaacccggcg tcgacacggt gctcgacgtg
     1801 cgcggccaga tctatctgcg ccagaacgta tcgaagctcg gaattgcacc gctgacgagc
     1861 atgtactact tcggcgagaa tcatcgcctc ggaatcgacg acttccgtcc ggaggtgcat
     1921 gactcggacg gcctgtcggt gcagtcgggc accggcgaat acatctggcg gcccctcgtg
     1981 aatccgaagc ggctgctcgt cacgtcgttc gccgtcgaca atccgctcgg gttcggcgtg
     2041 ctgcagcgcg accggcgctt cttcgattac gaggacctcg aagcgcgata cgaaacgcgc
     2101 ccgtcggtct ggatcgagcc gaagggcaac tggggcaacg ggcgcatcga gctcgtgcag
     2161 attccgacgc ccgacgagac gaacgacaat atcgtcgcgt acttcgtccc cgatgcgtca
     2221 ttggcgccgc tgcagccgta cgatttcgaa taccggatgc tctggcagaa ggaaaacgag
     2281 acgcgtccga cgctcgctta cgtggcgcag acccgccgcg gtcacggcta cctgcgcaag
     2341 cccgacgaca gcatcgcgtt catgatcgac ttcgaaggcg atgtcctgcg caatctgcct
     2401 gccgatacgc cggtgcaagg cgtcgtcagc accgactcga acgtcgagct cgtcgaggcg
     2461 aacgcctatc acaacgacgt gaccaatgga tggcggcttg cgcttcgcat caggcgccag
     2521 gacgacaaga agccgggcga gttgcgcgcc ttcctgcgca acggcggcac gacgctttcg
     2581 gagacatgga gctacatcct gccgaccaac tgagcgcggg cgtggcggcg ctgctgcgcc
     2641 gctaccttgc gcgcctcccg ctcgcggccg agcggcgcgc agcgctgctg gccgatgcgc
     2701 tgcaatgcgc acacgatgca cccgatgcac tcgggcgttt gcatgcggcg ctgtcggctg
     2761 ccgccgccaa ttcggcaaac gccgcgtacg cgacgatcgg tgcgcgcgtc cgcctcgcca
     2821 ccgcgcgcca gggcgcggcg gcggatgcga tccagtgcca cgacatccat gggcgcgaac
     2881 gactggagac gacgccccgc cttgcgcgaa gtccgatggc accgcacgca tggttcggcc
     2941 ggcgggcacg cgccgaagag gccgatcgcg tgccgtcggg tcgcgatcgg cgctcggccg
     3001 caaaaccgct cgaccccggc ggccgctggc gtcagcgtgc ggcgcaccgc cggctcgtgc
     3061 tcgtgacgct gatcgtcgtg cagacgctcg tggccaccga ttacatggcg ctcatcctgc
     3121 cgtatcacgg ccgccggccg ctcgaaatcg gcatcctcgt gctgttcggc gtgctgttcg
     3181 cgtggatctc cgccggattc tggacggcgc tcgcgggatt ctggctgctc gcgcgcgggc
     3241 acgaccgtta tgcgatctcg cggcgcatga tcggcgacgg ccccgcgtcg atacctggca
     3301 acgcgcgtac cgcgcttgtc atgccgatct gcaacgagga cgtgccgcgc gtgttcgccg
     3361 gcttgcgcgc gacgttcgaa tcgctgcgtg ccaccggcgc gctcgaccgc ttcaacttct
     3421 tcgtgctgtc cgacaccggc aacgccgaca cgcgcgtcgc cgagctcgac gcgtggctcg
     3481 cgctttgcaa tgcggtggac ggcttcgggc gcatcttcta tcgctggcgc cagcatcgaa
     3541 tcaagcgcaa gagcggcaac atcgccgatt tctgccgccg ctggggacgc aaatatcgct
     3601 acatgatcgt gctcgacgcc gacagcgtga tgagcggcga ctgcctgacg gcgctcgtgc
     3661 gcatcgccga ggccaatccc gatgcaggca tcatccagac ggcgccgcgg gcagccggcc
     3721 gcgacacgct ttacgcccgc gtccagcagt tcgccaccgg cgtgtacgga ccgcttttca
     3781 ccgcgggcct gcacgcgtgg cagttcggcg aatcgcatta ctggggacac aacgcgatca
     3841 tccgcgtcgc gccgttcatc cgctattgcg cgctcggacg cctgccgggc cacggcgcgc
     3901 tgtcgggcga gatcctgtca cacgatttcg tcgaggccgc gctgatgcgc cgcgcgggct
     3961 ggggcgtctg gattgcgtat gatcttcccg gcagctacga ggaaatgccg ccgaacctca
     4021 tcgacgagct ttcgcgcgat cggcgctggt gccagggcaa cctgatgaac ttccgcctgt
     4081 tctggatgaa gggactgcat cccgcgcacc gcgcggtgtt catgaccggc gtgctcgcgt
     4141 acctgtcggc gccgctgtgg ttcctgttcc tgatcctgtc gacggtgctg ctcgccattc
     4201 acacgctgtc cgagcccgcg tatttcaccg agccgtacca gctgttcccg atctggcccg
     4261 agtggcggcc cgaatgggca atcacgctgt tctcggcgac ggcgctgctc ctgttcctgc
     4321 caaagcttct cgccgggctc cgcgtcgcgt tcaccgatcc tgcgcgccat ggcggccgca
     4381 tgcacctcgc cgtgagtctc gccggcgaga tgctgctctc ggcactgctg gcgccgattc
     4441 gcatgctgtt ccacacgcag ttcgtgctga cgtcgcttgc cgggcggacc gtgcgctgga
     4501 agtcgccgcc acgcggcgac aacgaaaccg gatggcgcgc cgcgtttgcg cgccacggcg
     4561 tgcacacgtt cgtcggcatc gtctgggccg ccattgcctg gtggctgaat ccgtcgtacc
     4621 tgtggtggct gctgcccgtc gtgggcgcgc tcgcgctgtc gattccgttg tccgtcgtga
     4681 cgagccgcgc cggactcgga cgcaagctcc gccgacgcaa gtatttcctg attcccgagg
     4741 agtccgagcc ccccgacgtc atccgcgcca cgcgccggca ctggcaccgc gcggccgcgc
     4801 ccgccgattt cgctgcggcg gtcgtcgatc cgatcgtcaa cgcgctcgtg tgcgcgcagg
     4861 ccgtttcgcg cccggtgacc ggctggccgc gcgaggcgac cgccgacgca ctcgtcgagc
     4921 gcgcattgcg ccagggcctc gacgcactga cgccggccga tcgcggccgc ctgctcgccg
     4981 atcctgcggc gctgtccgcg ttgcacttcg cggtgtggac gtcgccggcg gcgcacctgt
     5041 catggcgcga ggcggtcgcg cgccccgtcg cgcgctcggc ggcgccacgg ttgcacgcgc
     5101 acgaggaaag cgaagcaagc gggcccgggg cgggtctcgc cgcctcgcat ccggtgcacg
     5161 gctgaccagg taacgcgccg gcggcgatga cttctcccgc gaaccaccgc acgacggcgc
     5221 ttccccgcgc ggcaccgatg ccgcgcaggc tgccaggaac caatgcgccg gaagcggtcg
     5281 agccgctctt cgactcgatc tggcctttcc tcgcgatcgt catcgtgctg gtcgcgcttg
     5341 cgttcatcga tttcctgctc gtgcaacccg cgccttcggc gcgcatcacg ttgcttgcgc
     5401 tgcgccttgg ctcgagcgcc gcgttgctga tcctcgcggg gcttacgttg cggcgcctct
     5461 tcgcacagcg cgcgcgtttg cagcaggcgc ttcgcgcaag cgaggagcgg ctcgtgagct
     5521 cgctgtcgga catccagatg cgcaagcagg ccgaagcact gctcgccgag gagaaggagc
     5581 gcgcgcaggt cacgctcgcg tcgatcgccg acgccgtggt caccgtcgat acctccgggc
     5641 ggatcgaatt cctgaactcg gccgcggagc gcgtcaccgg ctggacgctt gccgaagcgc
     5701 ggcagcgccc ggtggccgaa gtgttcgccg tcgtcgacga ggcaacgggt gcgccgattc
     5761 ccgacccggt cgggcgcgca ctcaccgatg gtgccatcgt cgaggcggag ggcaacgtcg
     5821 tgctgctgtg ccgcggcgcc gagtcgatcg cgatcgacta cagcgtcgcc ccgatccggg
     5881 atcgtgcagg caacagcgtg ggcgccgtgc tggtgatcca ggatatgagc cgcgagcggc
     5941 agtatgcggc gcgcctttcc aacctcgcga gccacgacgc gctcaccgga cttctcaacc
     6001 ggcgcgaatt cgagcagcgg gtgcgcgcga tcgtcgagca gcgcgagggg ccggaaggcc
     6061 agcacgcggt gctctacctc gacctcgatc agttcaaggt ggtgaacgac acgagcgggc
     6121 acgcggccgg cgacgagctg ctgcgccagg tcggtgcgct attgcggccg cgtctgcgcg
     6181 agggcgatgt gctcgcgcgg ctcggcggcg acgaattcgg cgtgctgctg ccgcattgcc
     6241 cgccggcacc ggcgctgcgc atcgccgagg cgttgcgcaa ggccatcatc gacttccgtt
     6301 tcgcatggaa gaatcgctcg ttcacgatcg gcgtcagcat cggcctcgtc aacctcgcgg
     6361 acggaccgca cacgctcgcg agtgtgctgt cggcggccga cgccgcgtgc tacctcgcga
     6421 aagacaaggg ccgcaaccgt gtgcaggtct accggcccga ggacagcgag gtcacgctgc
     6481 gccgtggcga gatggaatgg gtgcaccggc tgcaccgcgc gctcgccgag gatcggctgt
     6541 gcctctactc gcaaccgatg cgcgcgatgc atgccccggg tccgcacgtg c
//
LOCUS       DASSVX010000266         7108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1145092, whole genome shotgun sequence.
ACCESSION   DASSVX010000266 DASSVX010000000
VERSION     DASSVX010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145092"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFO53_11370"
     CDS             complement(<1..534)
                     /locus_tag="VFO53_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HET8876920.1"
                     /translation="MPIAAFTAYGIELEYMIVDRDSLAVRPIADRLLSVLADTPGPGA
                     GAEKAAVEWSNELVRHVVEVKNPLPVRDLASLPARFHDEIRDANRVLARENARLMPTA
                     MHPWMNPRRETWLWDGAIYRNYHRIYDCRGHGWANLQSMHINLPFGDDGELARLHAAI
                     RLVLPLLPALAASSPIAD"
     gene            complement(555..2027)
                     /locus_tag="VFO53_11375"
     CDS             complement(555..2027)
                     /locus_tag="VFO53_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HET8876921.1"
                     /translation="MSLLVVVNHPRDWPFEIAGTRIVTARDYLTDPAYSENQSARVLN
                     VCRTDRYQGRGYYVSLLAEARGHRPLPEVKTLGDLQSSEPPDRLLPATLCEQLQRALS
                     ANGEATFALDAFFGRDPAKQNDGLAQQLFAALRIPLLRAHFQRTRDRWQLRDLSILAV
                     SDIEPARRPLIADAARDYVTRASHPRAKALSSGEPSIAILFNENEPQPPSNPEAIEKL
                     RAAARALGMRASVIGRDEIDRLPQFDGLFIRDTTNVNHYTYQFARRAAAEGLVVVDDP
                     DSILKCTNKVYLNELLSRHRVPVPKTLMVHRDNVDRIVPLLGLPCILKQPDSAFSLGV
                     ARIETANAVHDALAWLFAKSDLVIAQEWLPTAFDWRIGVYDRRPLYVCKYFMAPGHWQ
                     VVKRESGRTLEGSTQALSVGEAPEIVVRTAVRAANLIGDGLYGVDLKQDGRQCYVMEV
                     NDNPNLDAGHEDAVLKDALYREVMGVFLRRIRERGRTTAS"
     gene            complement(2160..2843)
                     /locus_tag="VFO53_11380"
     CDS             complement(2160..2843)
                     /locus_tag="VFO53_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876922.1"
                     /translation="MPQPDETTCGPTCLHAIYAYWGDVEPLPSVIERMWRLEHGGTYA
                     VYLGCDALRNGYRARIYTYNLTVFDPTWFVAPGVDIASRLVVQREAKDERRGQHAVDG
                     YLEFLARGGRLRFANLSEHLIRRILHCRLPILTGLSSTYLYRSARECADGTADDVRGH
                     PAGHFVVIAGWDERRRRVMVVDPYQPNPWGPSHEYWIGVDRVVAAILLGIVTHDANLL
                     VVYPARGKR"
     gene            3315..3824
                     /locus_tag="VFO53_11385"
     CDS             3315..3824
                     /locus_tag="VFO53_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET8876923.1"
                     /translation="MLQCAACAGLAAAPFGDAQGTIFKCTDASGRILYSDAPCPGGSR
                     GEVVAPDENSPIPATSAPAPQRNGAAAVRTATDEPAQQAGDRYEPSVNERQRIVNLEQ
                     VQRAGDNAEKRQAARMEIDETRRGTVARMSYDDRRRKDGYWVDLGNLDQRRRAVAVQQ
                     LAALFAAYQ"
     gene            3855..4961
                     /locus_tag="VFO53_11390"
     CDS             3855..4961
                     /locus_tag="VFO53_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET8876924.1"
                     /translation="MASGITAAELRRYAIARSLFAPVGLQPAIERLGFVQADPIRAPA
                     RAQDLALRHRVAGYRAGDLDRGYPALDVEEDFVINYGFVPRAVYALMHPRTVRPAWSA
                     SRRKRAQAVLDFIRERGDVHPRDVDAHFAHGAVVNAWGGSSNATTHLLDAMHYRGWLR
                     VVRREAGIRIYAAREFMRAAAPAASAHARLDALVDMVVREYAPLPAQRLSPLVRRLRY
                     AAPQLARGIDDALWRAKRRLAHARVDGADWYWPADETPREIADALDGEVRLLAPFDPI
                     VWDRVRFERFFGWAYRFEAYTPPSRRKLGYYALPLLHGDAIPGWANLTMTNGALASDI
                     GYVRGTAPRGHAFARALDDELARMHVFLSVRAAE"
     gene            4958..5842
                     /locus_tag="VFO53_11395"
     CDS             4958..5842
                     /locus_tag="VFO53_11395"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8876925.1"
                     /translation="MNAVALFARAWRRHGLGASIIKALARTLHRRVLIEGVDDDFPRP
                     SPPRDLSMPTAEDAHAWHMGYGGRWKHVLPLPNDRMMLHVGAQSVDNFFFVGDAWAQL
                     LSRYIAPGSHVMDVGCGCGRTARFLVNNPHVQRFTGFDVFAPYVDWCNRFFGELHPGR
                     FAFRHVDVRTARYNPAGTLACADVHFPADDGDVDLVYAASLFTHLYPDDARAYAHEMR
                     RVLASGGLAVASFHDRPERGREFSGSEHRADYDASAFQRMFVEAGLELVEDVGDVCGQ
                     RTLVLRKPRGAATISGTA"
     gene            complement(5863..>7108)
                     /locus_tag="VFO53_11400"
     CDS             complement(5863..>7108)
                     /locus_tag="VFO53_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HET8876926.1"
                     /translation="PLLWDGYPMARQGKPAQALGWTLLAALWGGLITCVSAVVLAVPF
                     AHFALRFDSPEYFAVVLLGLSGVVALGGASMAKALISLCIGLLLATVGVDDTYGVDRF
                     TFSVPILKDGIEYLTVMVGAYGLGEVLTRMEQGFASPSIESGRKVETRLPRWREIWPL
                     RPTIVRSSLIGILCGIVPGAGATVGSFVAYGIEGQYGRRRADLGTGIPEGIVAPQVAS
                     TATVGGHMVPLLALGIPGSGATAVILGAFLLHGVQPGPQIFQTEAAMVYTIFASMFAA
                     VLGMCLLGYFAIKPLIRVLQLPEAVTSAFVALFCFVGAYSARNSLTDLWMIVIFGIVG
                     YLFERFRFPIAPMVLGCILGPIAETSFMTSMIAYQNDWTIFFTRPISGTVMALTVLAL
                     LYPAIRYVRRTMRAPRTTVRHA"
BASE COUNT         1140 a         2396 c         2463 g         1109 t
ORIGIN      
        1 gtcggcgatc ggcgagctcg cggcgagggc ggggaggagc ggcaggacga gcctgatcgc
       61 cgcatggagc cgcgcgagct cgccgtcgtc gccgaacggc aggttgatgt gcatgctctg
      121 caggttcgcc cagccgtgtc cgcgacaatc gtagatgcgg tgatagttgc gatagatggc
      181 gccgtcccag agccacgttt cgcgccgcgg gttcatccac ggatgcattg cggtgggcat
      241 gagccgcgca ttctcgcgcg caagcacgcg attggcgtcg cggatttcgt cgtgaaaccg
      301 cgcaggaagc gatgcaaggt cgcgcaccgg gagcggattc ttgacctcga cgacgtggcg
      361 caccagctcg ttcgaccatt ccaccgcggc tttctcggca cccgcgcccg gtccgggtgt
      421 gtcggcgagc acgctcagca gccgatccgc gatcggacgt accgcgagcg agtcgcggtc
      481 gacgatcatg tactcgagct cgatgccgta cgcggtgaac gccgcgatcg gcacctgcgt
      541 cgcgtcgctc ccgctcagga ggccgtcgtg cgcccgcgct cccggatccg gcgcaggaaa
      601 acgcccatca cctcgcggta gagcgcgtcc ttcagcaccg cgtcctcgtg gcccgcgtcg
      661 agattcgggt tgtcgttgac ttccatcacg tagcattgcc gcccgtcctg tttgaggtcg
      721 acgccataaa gtccgtcgcc gatcaggttc gccgcgcgca cggcggttcg cacgacgatc
      781 tccggggcct cgcccaccga aagcgcctgt gtcgatcctt cgagcgtgcg gcccgattcc
      841 cgtttcacga cctgccagtg accgggggcc atgaagtact tgcacacgta gagcgggcgc
      901 cggtcgtaga cgccgatgcg ccagtcgaac gcggtcggca gccattcctg ggcgataacg
      961 agatcggatt tcgcgaacag ccacgcaagc gcatcgtgca ccgcattcgc ggtttcgatc
     1021 ctggcaacgc cgagcgagaa cgcgctgtcg ggctgcttca ggatgcacgg gaggccgagc
     1081 agcggcacga tccggtcgac gttgtcgcgg tggaccatca gcgtcttcgg caccggcacg
     1141 cggtgacgcg acaggagctc gttcaggtaa accttgttcg tgcacttgag aatcgaatcg
     1201 ggatcgtcga cgacgaccag gccctcggcg gccgcacggc gcgcgaactg gtaggtgtag
     1261 tggttgacgt tcgtcgtgtc acggatgaag aggccgtcga attgcggcag gcggtcgatt
     1321 tcgtcccggc cgatcaccga cgcgcgcatt cccagcgcgc gtgctgccgc acgaagcttc
     1381 tcgatggctt cggggttgga cgggggttgc ggctcgttct cgttgaaaag aatcgcgatc
     1441 gacggttcgc cgctcgacag tgctttcgca cgcgggtggc ttgcgcgcgt cacgtagtcg
     1501 cgcgccgcgt cggcgatcag cggccggcgc gccggctcga tgtcggacac ggccaggatc
     1561 gacagatcgc gcaactgcca gcgatcgcgc gtgcgctgga agtgcgcgcg aagcagcggg
     1621 atgcgaagcg cggcaaagag ctgttgcgca agaccgtcgt tctgcttcgc cggatcgcgg
     1681 ccgaagaacg catcgagggc gaacgtcgcc tcgccgttcg cagacagcgc gcgctgcaat
     1741 tgctcgcaca acgtggcggg gagcagtcga tcgggcggct cgctcgattg caggtcgccg
     1801 agcgtcttca cctcgggcag cggccggtgg ccacgtgcct cggcgagcag cgacacgtaa
     1861 tagccgcgcc cctggtagcg atcggtgcgg cagacgttca gcacgcgcgc cgactggttc
     1921 tcgctgtacg cgggatcggt caggtaatct cgcgcggtca cgatgcgcgt gcccgcgatc
     1981 tcgaacggcc agtcgcgcgg atgattgacg acgacgagca agctcatctg tcggccccca
     2041 cgctcgcggc tcgggacggc ccggcgccgc tcatctgtgg gcccccacgc tcgcggctgt
     2101 cgccgcttcg ctgcccccca agggggagca attcgcggct tgggacggcc cggcgccgct
     2161 catcgctttc ctctggccgg gtagacgacg agcaggttcg cgtcgtgcgt gacgatgccg
     2221 agcaggatgg ccgccacgac ccgatcgacg ccgatccagt actcgtgcga cggaccccac
     2281 ggattcggct gatagggatc gacgaccatc acgcgccgcc gccgctcgtc ccagccggcg
     2341 atgacgacga aatggccggc cggatggccg cgaacgtcat ccgcggtccc gtccgcgcat
     2401 tcgcgcgccg agcgataaag gtacgtcgac gagaggccgg tcaggatcgg aagccggcaa
     2461 tggagaatgc gccggatcag gtgttccgac aggttcgcga agcgaagccg gccgccgcgc
     2521 gcgaggaatt cgagatagcc gtcgactgcg tgctgcccgc ggcgctcgtc cttcgcttcg
     2581 cgctgcacca cgaggcgcga ggcgatgtcg acgcccgggg ccacgaacca cgtcgggtcg
     2641 aacacggtga ggttgtacgt gtagatgcgg gcgcgatagc cgttgcgcag tgcatcgcat
     2701 ccgaggtaga cggcataggt cccgccatgc tccagccgcc acatgcgctc gatcacgctc
     2761 ggcagcggct cgacgtcgcc ccagtacgca tagatcgcat gcagacaggt cggaccgcag
     2821 gtggtctcgt cgggctgcgg cagagtcgtt accggaaggc gaagcgagcg cgtcgcgcac
     2881 gcgagatcgg cggcggtcgg cgccggccgc gccgtgccgg gaacctcggt gttacgggcg
     2941 tcccggccgc gcgcgctctc tgcgacgttg cgaagctgca aaggatgagg agatgccccg
     3001 gcgcccgatc aggcgacggc gcggcggggc gatctcgcag gaggcatgtg gccactattg
     3061 cgggattggc gttgcggcga atccggccgc gcgctgaaag gcatgtacgc gtcggtccga
     3121 cgtccgcgga cggcgacgac ggcgtcgatg ccgctccacg accgtgcggc ctgcccgtcg
     3181 cgtcccggca ccgggcaatg gctacaatcc gcgccagggt cgagcgaact acgggagacg
     3241 ggcgaatgcg aacgaaaagc gggaacccgc ggcgcgcttc atcgcgccgg ggtcggacgg
     3301 cgcttgcgac ggcgatgctt cagtgcgccg cgtgcgcggg ccttgctgcg gcaccgtttg
     3361 gcgatgcgca gggcaccatc ttcaaatgca ccgacgcttc gggccgcatc ctctatagcg
     3421 atgcgccgtg tccgggcggc tcccgcggcg aagtcgtcgc gcccgatgaa aattcgccga
     3481 ttccggcgac aagcgcaccc gcaccgcagc gcaacggtgc cgctgccgtc cgcaccgcga
     3541 cggacgaacc ggcgcagcag gccggggatc gctacgagcc gagcgtcaac gagcgccagc
     3601 gcatcgtcaa tctcgagcag gttcagcgcg cgggtgacaa cgccgagaag cgccaggcgg
     3661 cgcgcatgga gatcgacgag acgcggcgcg gtaccgtcgc gcggatgtcg tatgacgacc
     3721 ggcgcaggaa ggatggttac tgggtcgatc tcggaaatct cgatcagcgg cgccgtgcag
     3781 tagccgtgca gcagctcgcg gcgctctttg ctgcgtatca gtgacaccgg gcacgacgtt
     3841 tcaggatcga cgacgtggca tccggcatca ccgccgccga gcttcgccgc tacgcgatcg
     3901 cgcgcagtct cttcgcgccg gtcggcctgc agccagcgat cgagcgtctc ggcttcgtgc
     3961 aggccgatcc gatccgcgcg ccggcacgtg cgcaggacct cgcgctgcgc catcgcgtcg
     4021 ccggttatcg tgcaggagac ctcgatcgcg gctaccccgc gctggacgtc gaggaggatt
     4081 tcgtcatcaa ctacggcttc gttccacgcg ccgtttacgc gctcatgcat ccgcgcacgg
     4141 tgcgccctgc atggagcgct tcacggagga agcgcgcgca agccgtgctc gacttcatcc
     4201 gcgagcgcgg cgacgtgcac ccgcgcgacg tcgacgcgca ctttgcgcac ggcgcggtcg
     4261 tcaatgcatg gggcggcagc tccaatgcga cgacgcatct gctcgacgcg atgcactatc
     4321 gcggttggct gcgtgtcgtg cggcgcgagg cgggcatcag gatctacgcc gcgcgcgagt
     4381 tcatgcgcgc ggccgcgcca gcggcgtcgg cgcacgcccg gctcgatgcg ctggtcgaca
     4441 tggtcgtgcg cgagtacgcg ccgctgcccg cgcagcgcct gtcgccgctc gtgcggcggc
     4501 tgcgctacgc cgcgccgcag ctggcgcggg ggatcgacga cgcgctctgg cgcgcgaagc
     4561 ggcgtcttgc gcacgcccgc gtcgacggtg cggactggta ctggccggcg gacgagacgc
     4621 cgcgggagat cgccgatgcc ctggacggcg aggtgcggct gctcgcgccg ttcgatccga
     4681 tcgtatggga tcgcgtgcgc ttcgagcgct tcttcggctg ggcgtatcgc ttcgaggcgt
     4741 acacgccgcc gtccaggcgc aagctcggct actatgcgct cccgctgctg catggcgatg
     4801 caatccccgg ctgggcgaac ctgacgatga ccaacggcgc gctggcaagc gacatcggtt
     4861 acgtgcgcgg tactgcgccg cgtggccacg ccttcgcgcg cgcactcgac gacgagcttg
     4921 cgcggatgca cgttttcctg tcggtgcggg ctgcggagtg aacgccgttg cgctcttcgc
     4981 ccgcgcctgg cggcggcatg gccttggtgc cagcatcatc aaggcgctcg cgcgcacgct
     5041 gcatcgacgc gtgctgatcg aaggcgtcga cgacgacttt ccgcgcccat ccccgcctcg
     5101 cgacctgtcg atgccgaccg cggaggatgc gcatgcctgg cacatgggct acggcggacg
     5161 ctggaagcac gtgctgccgc tgccgaacga ccggatgatg ctgcacgtcg gcgcgcagag
     5221 cgtcgacaac ttctttttcg tcggcgatgc gtgggcgcaa ctgctgtctc gctacatcgc
     5281 tcccggaagc catgtgatgg acgttggctg cggctgcggc cgcacggcgc gcttcctcgt
     5341 gaacaatccg cacgtgcagc ggttcaccgg cttcgacgtg ttcgcgccgt acgtcgactg
     5401 gtgcaaccgc tttttcggcg agcttcatcc gggccgcttc gcgttccgcc acgtcgacgt
     5461 gcgcaccgcg cgctacaacc cggccggcac gctggcatgc gccgacgtgc attttcccgc
     5521 ggacgacggc gacgtcgacc tcgtgtacgc ggcgtcgctc ttcacgcatc tgtatcccga
     5581 cgacgcgcgt gcttatgcgc acgagatgcg gcgggtgttg gccagcggtg gtctcgccgt
     5641 ggcgtcgttc cacgatcgcc cggagcgtgg gcgcgaattc tccggcagcg agcatcgcgc
     5701 cgattacgac gcctcggcgt ttcagcggat gttcgtggag gcgggcctcg agctcgtcga
     5761 ggacgtcggc gacgtgtgcg gacagcggac gctggtgctg cggaagccac gcggtgctgc
     5821 cacgatcagt ggcacggcgt agcggccacg gcagctcgcc ttttacgcgt gcctcaccgt
     5881 cgtccgcggc gcgcgcatcg tgcgccgtac gtagcggatc gcgggataca gaagtgccag
     5941 caccgtcaac gccatcaccg tgccgctgat cggccgcgtg aagaagatcg tccagtcgtt
     6001 ctggtaggcg atcatgctcg tcatgaacga cgtttcggcg atcggaccga gaatgcagcc
     6061 gagcaccatc ggcgcgatcg gaaagcggaa gcgctcgaac aggtagccga cgatgccgaa
     6121 gatgacgatc atccacaggt cggtcaggct gttgcgcgcg ctgtacgcgc cgacgaagca
     6181 gaacagcgcg acgaacgccg aggtcaccgc ctcgggaagc tgcagcacgc ggatcagcgg
     6241 cttgatcgcg aagtagccga gcaggcacat gccgagcacc gccgcgaaca tcgacgcgaa
     6301 aatggtgtag accatcgccg cctcggtctg gaagatctgc ggcccgggct gcacgccgtg
     6361 cagcaggaac gcgccgagga tcacggccgt tgcgccgctg ccgggaatgc cgagtgccag
     6421 gagcggcacc atgtggccgc cgacggtcgc cgtcgacgcg acctgcggcg cgacgatgcc
     6481 ttcgggaatg ccggtgccga gatccgcacg tcgcctgccg tactgtccct cgatgccgta
     6541 agcgacgaac gagccgaccg tagcaccggc gcccggcacg atcccgcaca ggatgccgat
     6601 cagcgagctc cgtacaatcg tcgggcgcaa cggccagatc tcgcgccagc gcggcagccg
     6661 cgtctcgacc ttcctgccgg actcgatcga cggcgaggcg aagccctgtt ccattcgcgt
     6721 cagcacctcg ccgaggccgt aggcgccgac catcaccgtc aggtactcga tgccgtcctt
     6781 caggatcgga acgctgaacg tgaaccggtc gacgccgtac gtatcgtcga caccgaccgt
     6841 ggcaagcaga agcccgatgc acaggctgat cagcgccttc gccatcgatg caccgccgag
     6901 cgcgacgacg ccggacagcc caagcagcac gacggcgaaa tactccggcg aatcgaagcg
     6961 cagcgcgaaa tgcgcgaacg ggaccgcgag caccacggcc gacacgcagg tgatgaggcc
     7021 gccccacagc gccgcaagca gcgtccagcc cagcgcctgt gcgggcttgc cctggcgcgc
     7081 catcgggtag ccgtcccaca acaacggc
//
LOCUS       DASSVX010000267        15284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_861663, whole genome shotgun sequence.
ACCESSION   DASSVX010000267 DASSVX010000000
VERSION     DASSVX010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 15284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15284
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861663"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFO53_11405"
     CDS             complement(<1..617)
                     /locus_tag="VFO53_11405"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876927.1"
                     /translation="MSAAPALEFRGVGKVFETPTGALRALADIDFELRRGEFLSVLGP
                     SGCGKSSLLALATGLEFPTEGSVEVDGRRVERPVTDVGIVFQTDVLLDWRTVLGNVML
                     QIEMRGLDAAQYEGRARALLHTVGLAGFEAKRPYELSGGMRQRVSICRALVHDPHILF
                     MDEPFGALDAITREQLVLELHRIWNETRKSVMFVTHDIGEAIFLA"
     gene            complement(614..1498)
                     /locus_tag="VFO53_11410"
     CDS             complement(614..1498)
                     /locus_tag="VFO53_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876928.1"
                     /translation="MTAMTGQQTKAARGAPPADVPAPVRAPARAAKRAWLSRASPLVV
                     IAALLVVWEVAVRAFGVPAYLLPPPSAIARRMVIDHRLLAVNAWVTLGEVLAGYALAI
                     IVSIPLAAVLAQFRVVENAIYPTLVASQTIPKVAIAPILVVWFGFGLTPKILIAFLIC
                     FFPIVVDTMTGFRCVPREVIWLARSMGASQWKTFMKIRIPAALPNIFAGLKVASTLAV
                     VGAVVGEFIGADRGLGYQLIVANGLMDVQLSFAVLVALSLLGIGLYAGVDLLERLALP
                     WHVSHRVTAATPAAEKRQ"
     gene            complement(1495..2589)
                     /locus_tag="VFO53_11415"
     CDS             complement(1495..2589)
                     /locus_tag="VFO53_11415"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET8876929.1"
                     /translation="MTNPRFRTELPRLYESEISPTIASARFGLDPAQILDFSLNVNPF
                     GPPASAVAAARAALERCNAYPDTRLSALRAALARRHAVDEDQLFFGAGLDDVIKLVLQ
                     AWAGDGDGVVVHVPTFPRYELEARLCGATPIAVQGDPPWTIDVDAIRAPLAKGGVGVA
                     FLCTPNNPTGATIATPAIRAMARDFRDTILIVDEALGNPLDDGAIALARSEPNVVVLR
                     TFSKAFGLAGLRIGYAVGPTALLAVAERGRPPFNVTAPAETAALAALDDSAFVTQSYE
                     TFGAEAQRFADAIDRIPAFALRGRHANMLLIEVKSGTTVDCIDRLAAQGLLVADGACF
                     GSGHECAAIRVSLRDRAANTRLLAALAAPG"
     gene            complement(2586..3341)
                     /locus_tag="VFO53_11420"
     CDS             complement(2586..3341)
                     /locus_tag="VFO53_11420"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleate cis-trans isomerase"
                     /protein_id="HET8876930.1"
                     /translation="MIASADNANVMRRNPRLDGRRVGLIVPSVNATIEPEFAWIAPPG
                     LSFHAARIPLRETTPGGLREMNEGVETAARLLASVSPDVVAYACTSGSFLEGGERLRA
                     RIDAIGAVVGCPVVATSAALVDALHALGVRRLALATPYLDSINRVEQRFLEDHAFDVV
                     STRALGLSGAAIRDVSPERVFELACEADCDAADGVFVSCTDLRALEVVEALEARLSKP
                     VLTSNQVTLWALLRVLGSKADIAGFGRLLRQQR"
     gene            complement(3338..4363)
                     /locus_tag="VFO53_11425"
     CDS             complement(3338..4363)
                     /locus_tag="VFO53_11425"
                     /inference="COORDINATES: protein motif:HMM:NF025694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate cyclase domain-containing protein"
                     /protein_id="HET8876931.1"
                     /translation="MTIELARIAEAIDHLITAPMSNWTILKGIPLDRLYEASRRQATQ
                     PLTLAAADLLATRVKPGDRVLMVSGFIMRDYGLPETDGPIGAAVLARALALGLGAVPI
                     GVSEASVLPCLEACFAAAGLIPATVEALGRGRNRCAFVDFPVDASFAAAKARRILDEL
                     QPKALIAVERPGAGADGEYHGGGGFAISSFTAKTDVLFALAQERGIATIGIGDLGNEL
                     GLGVVAAEVREHVPLGETIAAQLAADVAVIANISNWGAYGVAACLAAMLGNDGLFHDG
                     REELRLIDACVAAGAIDPVGGQRRAWVDGTDAATNAAMVDLLRSVVVLSQREKVNIDG
                     YRSSWRA"
     gene            4532..5383
                     /locus_tag="VFO53_11430"
     CDS             4532..5383
                     /locus_tag="VFO53_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET8876932.1"
                     /translation="MTRTRVDGGRHAGNGASRAPGTSPRTPHVLAVRHAVEVLRCLAS
                     DTPLLGVSEIASVVGMHKSSVSRMVATLEEDGLVERDPASKRIRLGPGLLSLAAPLLA
                     SVRVIETAQPQLVELAQRCGETISISVWDGAGAVNLAQVLGGKAVKHYAAPGSRNPAH
                     ASASGKLLLAFASADTAERILRRELRRFTPRTLCTRSALLAEIDTIRRQGYAVNEGEL
                     SLDVGGLAAAIRNDNANVVAAITATVPMYRFATTNRRQLVELVRDAADRVSARLGYAR
                     ADHHANR"
     gene            5429..6067
                     /locus_tag="VFO53_11435"
     CDS             5429..6067
                     /locus_tag="VFO53_11435"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HET8876933.1"
                     /translation="MARITLLRATDYARQRWKNGGGTTTELARNGNADRWLWRLSVAD
                     VERSGPFSDFTGYRRLIVLLDGRGMALSFDAAAPVVLDERYRPFAFDGGARTHCDLLD
                     GPIRDMNLIFDATRIDASLDVCTLAQGASLRVAASECALLHAIGGRFDVTIGDDSAAL
                     SPGDTLQVGEREAIPITATALDAHAVLAQVAIGYRPCPSCRSPGVGVDATRC"
     gene            6100..7785
                     /gene="hutU"
                     /locus_tag="VFO53_11440"
     CDS             6100..7785
                     /gene="hutU"
                     /locus_tag="VFO53_11440"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HET8876934.1"
                     /translation="MNDSSDSRHAARSIRAPRGAELTCANWPIEAAYRMIQNNLDREV
                     AENPDALVVYGGIGKAARNWACFDAILASLRRLKPDESLLIQSGKPVGIFRTHADAPR
                     VLIANSNLVPKWATWEHFDELDRKGLMMFGQMTAGSWIYIGSQGIVQGTYETFVEAGR
                     RHYGGDLRGRWILTAGLGGMGGAQPLAATFAGACSLNVECQQSRIDARLRTRYLDEQA
                     ADLDDALARVTRYTSQGRAASIGLLGNAAELLPEIVRRGITPDLLTDQTSAHDLVNGY
                     LPPGWSVERWKAAQADPLQHAALREAAARGCATHVRAMLSLQAVGVPTFDYGNNIRQV
                     ALDQGVANAFDFPGFVPAYIRPLFCRGKGPFRWVALSGDPEDIRKTDAKLKLLFAQDA
                     HLHRWLDMAGERIAFQGLPARICWLGLGERHRAGLAFNEMVRTGELKAPIVIGRDHLD
                     SGSVASPNRETEAMRDGSDAVSDWPLLNALLNTASGATWVSFHHGGGVGMGYSQHAGV
                     VIVCDGTDAAAQRIERVLWNDPGSGVMRHADAGYDDAIACARESGLDLPMIGA"
     gene            complement(7829..8092)
                     /locus_tag="VFO53_11445"
     CDS             complement(7829..8092)
                     /locus_tag="VFO53_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876935.1"
                     /translation="MKRILVGTALALFGLAPAIGSACEYNDASMASSTPAAQIGLATP
                     PAASKAPAPVVAKAAPATQAKQASDKATTPAPTAKLAAVANRN"
     gene            complement(8227..8481)
                     /gene="infA"
                     /locus_tag="VFO53_11450"
     CDS             complement(8227..8481)
                     /gene="infA"
                     /locus_tag="VFO53_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET8876936.1"
                     /translation="MAKEEVLQMNGVVAEVLPDSRYRVTLDNGHSLVAYSAGKMRKHR
                     IRIIAGDKVALEISPYDLSKGRITFRQLETRVSTPPRPRY"
     gene            complement(8578..8784)
                     /locus_tag="VFO53_11455"
     CDS             complement(8578..8784)
                     /locus_tag="VFO53_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET8876937.1"
                     /translation="MATGTVKWFNDSKGFGFVTPEDGGKDLFAHFSAIQGQGFKTLKE
                     GQRVSFDVTTGPKGLQAANIRSVD"
     gene            9026..10000
                     /locus_tag="VFO53_11460"
     CDS             9026..10000
                     /locus_tag="VFO53_11460"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET8876938.1"
                     /translation="MLVRGTFLLLLGTLALAANGANAAPGGFPSRPLTLVVGFAPGGG
                     IDIQARLLAKGLERYFGQPVIVENRPGAGSRIASEHVARASPDGYTLLVTAAAAAIDM
                     ALSANAGVDKLHDFAPVSTISSSPLILVVNPSVPVADVQALVRRARSLPGMLNYSSSG
                     AGTTGHLYGELFKLRTRTDVVHVPYKGSAPALASLLAGDVEMTFASSASVMQFVKAGR
                     ARALATTGARRSPLLPDVPTIEEAGVANAQADIWYGVLAPAATPPEVIEALAKATLSV
                     TTSAEFSQRMLGLGVEPSANTPGAFRVLLQKEVARWAEVAKAAHIQAD"
     gene            10003..12591
                     /locus_tag="VFO53_11465"
     CDS             10003..12591
                     /locus_tag="VFO53_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET8876939.1"
                     /translation="MASQPDTRSVGARLAEVASRVPDHPAIVERDACMTYAALDSLAT
                     AIAWRIVATRESRPRAVCLLFERRLATIAALFGAARSGRPYVPLDAGDPEARLRFILG
                     DCQPIALLTETALEGRARAIAPGGCEVIAIDALEPGGDADALPAVASDAPVYLHYTSG
                     STGQPKGISQNHRNVLFLADAYAKALRIGAADRLSLLYTMSFAAANNAVFRALLHGAT
                     LCAYDLRRGGIAALPDWLDRQRITVLHLVPTAFRELATRLATDRLLPHLRAIHLGGEL
                     VFASDVELFRRHTLDHCILVNQLASTEAGPVAQGIVDHWTPVAPGTVVPVGRCIDGVR
                     VEIRRDDGALAGIGEVGELVVCSRYVSPGYWRRPELDAAAFSPDPADAEARQFRSGDL
                     GHVDAAGNLHFLARKGNRVKIRGHSVDLTEIETALATCPGVATAAVTAIDDAPPAPVR
                     LLAGIVAADGAVRDPQIVRRHLATRLPMYMLPAQIVYVDALPFTASGKVDRLALAQIP
                     AAPAPPARDIEAPEDDVERKVAEVFEDLLEVAHAGRDDDFFLLGGDSLASAELQLRLT
                     EAFGVPVRNFQRDATVAAIAAGIRDAAAERTQSPQRLPVLMPLWQNGREPPLFLVHGR
                     HGQAFVSPHFMRLLGDEQPVWVFQARGLDGVSEPHASVEAMAAEYLAEMRRQRPQGPY
                     FLGSLCAGVYIVAAMARALRDAGERVLPLLLLDPPNSVLQPGYSQLTQAQFVDKMKAR
                     RAKGTTAGPMENPAYIDALLRTAMAFEHAIAKHRPLPYDGPVYVLSSYQRMRGGDSFD
                     LRRIFTGRYKRYEVGTTHAQSLDPRNPVFASALRRCVELIRDAARDRPAIAYRVQGGV
                     SSAEMP"
     gene            complement(12549..15200)
                     /gene="mdeB"
                     /locus_tag="VFO53_11470"
     CDS             complement(12549..15200)
                     /gene="mdeB"
                     /locus_tag="VFO53_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate dehydrogenase"
                     /protein_id="HET8876940.1"
                     /translation="MRDVQPDADPQETREWLDALEATVRTGGRERGLFLLKKLEEQAQ
                     HLRIVTHVPTYSAYQNTIPLEEQGAYPGDLALEERISAIVRWNALAMVLRANRAYGEL
                     GGHIASYASAAEIFEVGFNHFFRGAGAPHGADLVFFQPHSSPGVYARAFLEGRLSEGQ
                     LANYRQEVGGNGLSSYPHPWLMPGFWQFPTGSMGIGPISAIHHARFMRYLEHRGIATM
                     GDRHVWGVFGDGEMDEPESVAALTLAARERLDNLTFIINCNLQRLDGPVRGNGQIIQE
                     LESLFIGAGWNVIKVLWGSDWDALFARDTHHALLRQFAATVDGQYQTLGANDGAYNQS
                     HFFGLDPELRALVAHMTLAEIDALRRGGHDMRKLYAALHAAKAHPGQPTVVLAKTKKG
                     YGMGGAGESRMTSHQQKKLDLDALKAFRDRFRLTLSDDDLLEMRFHPLAADGPELAYL
                     RDRRAALGGPVPLRHRDAAQVPIPPIDAYARFALEAAGHEMSTTTAMVRLITNLLKDR
                     ALGPRIVPIVADEARTFGMANLFRQIGIYSPSGQLYEPEDAASMLRYREAKDGQLLEE
                     GITEAGALSSWVAAATAYSVHGVAMLPFYIYYSMFGFQRVGDLIWAAADQRARGFLLG
                     GTAGRTTLGGEGLQHQDGSSHVVAATVPNCRAYDPAFACELAVIVDHAARQMMERQDD
                     VFYYITVMNENYAQPSLAPGAASDIVRGMYRFAAASGAAQVRLLGSGAILREVIAAAD
                     LLRDDWQVASEVWSVTSFSELARDARDVERWNRLHPTAPAKSGHAAQCLAGSAPIVAA
                     TDYVRAWPQLIASYVDAPFVALGTDGFGRSDTRSALRGHFEIDRHHIAVAALHALAKR
                     STVPAATVASALQRYGISADDTPPWTR"
BASE COUNT         2479 a         5237 c         5165 g         2403 t
ORIGIN      
        1 tcggcaagga agatcgcttc gccgatgtcg tgcgtcacga acatcacgct cttgcgcgtt
       61 tcgttccaga tccggtggag ctccagcacc agttgctcgc gcgtgatcgc atcgagcgcg
      121 ccgaacggct cgtccatgaa caggatatgc ggatcgtgga cgagcgcgcg gcagatcgac
      181 acccgctggc gcatgccgcc cgaaagctcg tagggccgct tcgcctcgaa gccggcaagg
      241 ccgacggtgt gcaacagcgc gcgtgccctg ccctcgtact gcgcggcatc gaggccgcgc
      301 atctcgatct gcagcatgac gttgccgagg acggtccgcc agtcgagcag cacgtcggtc
      361 tggaacacga tgccgacatc ggtcaccgga cgctcgacgc gacgcccgtc gacctcgacg
      421 ctgccttcgg tggggaactc gagacccgtc gcgagcgcca gcagcgagct cttgccgcag
      481 ccgctcggac cgagcaccga caggaactcg ccgcggcgca gctcgaagtc gatgtcggcg
      541 agcgcgcgca gcgcgccagt gggcgtctcg aacaccttgc cgacgccgcg gaactcaagc
      601 gcaggagcgg cgctcactgg cgcttctccg ctgcgggcgt cgccgcagtc acgcggtgcg
      661 atacgtgcca tggcaacgca agccgctcga gcagatcgac gccggcataa aggccgatgc
      721 cgagcaggct caacgcgacc agcaccgcga acgagagctg cacgtccatc aggccgttcg
      781 cgacgatcag ctggtagccg agcccgcggt ccgcgccgat gaactcgccg accacggcgc
      841 cgacgaccgc gagcgtgctc gcgaccttga gcccggcgaa gatgttggga agtgcagccg
      901 ggatgcggat cttcatgaat gtcttccact gcgacgcgcc catcgagcgc gcgagccaga
      961 tgacctcgcg cgggacgcag cgaaatccgg tcatcgtgtc gacgacgatc gggaagaagc
     1021 agatcaggaa ggcgatcagg atcttggggg tgagtccgaa gccgaaccac acgacgagaa
     1081 tcggcgcaat ggcgaccttc ggaatcgtct gcgacgcgac gagcgtcggg taaatcgcgt
     1141 tctcgacgac gcggaactgc gcgaggaccg cggcgagcgg gatgctgacg atgatcgcca
     1201 gcgcgtagcc cgcgagcact tcaccgagcg tcacccacgc gttgacggcg agcagtcggt
     1261 ggtcgatcac catgcgccgg gcgatcgccg acggcggcgg cagcagatac gccggcacgc
     1321 cgaacgcgcg cacggcgact tcccaaacga cgagcagcgc cgcgatgacg acgagcgggc
     1381 tcgcgcggga gagccatgca cgtttcgcag cgcgcgcagg tgcgcgaacc ggcgccggca
     1441 cgtcggcagg cggcgcgccg cgcgcagctt tcgtctgctg gccggtcatc gcagtcatcc
     1501 gggcgctgcg agcgccgcca gcagccgcgt gttcgccgca cgatcgcgga gcgacacacg
     1561 gatcgctgcg cattcatgcc cgctgccgaa acacgcgcca tcggcgacga gaagcccctg
     1621 cgctgccagc ctgtcgatgc agtcgaccgt ggtgccggat ttcacttcga tcagcagcat
     1681 gttcgcatgg cgcccgcgga gcgcgaatgc cggtatccga tcgattgcgt ccgcgaagcg
     1741 ttgtgcctcg gcgccaaacg tctcgtaact ttgcgtcacg aatgccgagt cgtcgagggc
     1801 cgcgagcgcc gcggtctccg cgggagcggt cacgttgaac ggcgggcggc cgcgctcggc
     1861 aacggcaaga agcgccgtcg ggccgacggc gtagccgatg cgcaggccgg cgagcccgaa
     1921 tgccttcgag aacgtgcgca gcacgacgac attgggctcg cttcgcgcaa gcgcgatcgc
     1981 cccgtcgtcg agcggattgc cgagcgcctc gtcgacgatg aggatggtgt cgcgaaagtc
     2041 tcgcgccatc gcgcgtatcg ccggcgttgc gatcgttgcg ccggtcggat tgttcggcgt
     2101 gcacaggaat gcgacgccga cgccgccctt tgcaagcggc gcgcgaatcg catcgacgtc
     2161 gattgtccac ggcggatcgc cctgcaccgc aatcggagtg gcgccgcaaa gccgcgcctc
     2221 gagctcgtaa cgcggaaacg tcggcacgtg cacgacgacg ccgtcgccat cgcccgccca
     2281 tgcctgcagc acgagcttga tgacatcgtc gagccccgcg ccgaagaaga gctgatcctc
     2341 gtcaacggcg tgccggcgcg ccagcgccgc gcgaagcgcc gacagccgcg tgtcgggata
     2401 cgcgttgcag cgctcgagcg cggcgcgtgc cgcggcaacc gccgacgccg gcgggccgaa
     2461 cggattcacg ttgagcgaga agtcgaggat ttgcgcggga tcgagcccga agcgcgccga
     2521 cgcgatggtc ggcgagatct ccgactcgta caggcgcggc agctccgtgc ggaagcgagg
     2581 attcgtcatc gctgctggcg aagcaggcgg ccgaatcccg cgatatcggc tttgcttccg
     2641 agcacgcgca gcagcgccca cagcgtcacc tggttgctcg tcagcaccgg cttcgacagc
     2701 cgcgcctcga gcgcctcgac cacttccagc gcgcgcaaat cggtacagct cacgaataca
     2761 ccgtcggccg cgtcgcaatc ggcttcgcag gcgagctcga acacgcgctc gggcgaaaca
     2821 tcgcggatcg cggcgccgga cagacccagt gcgcgcgtcg agacgacgtc gaacgcgtgg
     2881 tcttcgagaa agcgttgctc gacgcggttg atcgaatcga gatacggcgt cgcgagcgcg
     2941 agacgccgga cgccgagcgc gtgcaacgcg tcgaccagcg ccgccgacgt cgcgaccacc
     3001 ggacagccga cgacggcgcc gatcgcgtcg atccgcgcgc gcaaccgttc gccgccttcg
     3061 aggaacgagc cgctcgtgca cgcgtacgcc accacgtcgg gcgaaacgga tgcgagcaac
     3121 cgcgccgccg tttcgacgcc ttcgttcatt tcacgaagtc cgccgggcgt ggtctcgcgc
     3181 aacgggatgc gcgccgcgtg gaacgacagc ccgggcggcg cgatccacgc gaactcgggc
     3241 tcgatcgtcg cgttcaccga cggaacgatc aggccgacgc gacgaccgtc gagccgcgga
     3301 ttgcgccgca tgacgttcgc attgtcggcg ctcgcgatca tgctcgccac gagctccggt
     3361 agccgtcgat gttcaccttc tcgcgctggc tcagcacgac gaccgagcgc agcaggtcga
     3421 ccatcgcggc gttggtggcg gcgtcggtgc cgtcgaccca cgcgcgtcgc tggccgccga
     3481 ccggatcgat cgcacccgcc gcaacgcagg cgtcgatcag ccgcagctcc tcgcgtccgt
     3541 catggaaaag gccgtcgtta ccgagcatcg cggcaaggca cgcggcgacg ccatacgcac
     3601 cccagttcga gatgttggcg atcaccgcga catcggcggc aagctgcgcg gcgatggtct
     3661 cgccaagcgg cacgtgctcg cgcacctcgg cggcgaccac gcccagtcca agctcgttgc
     3721 cgaggtcgcc gatgccgatc gtcgcgatgc cgcgctcctg cgcgagcgcg aagagcacgt
     3781 cggtcttcgc cgtgaacgac gagatcgcga aaccgccgcc gccgtgatat tcgccgtcgg
     3841 cgcccgcacc cggccgctcg acggcgatca gcgccttcgg ctggagctcg tcgaggatgc
     3901 ggcgcgcctt ggccgcggcg aacgaagcgt ccaccgggaa gtcgacgaag gcgcaccggt
     3961 tgcgtccgcg tccgagcgct tcgacggtcg cgggaatgag cccggccgcg gcgaagcacg
     4021 cctcgaggca cggcagcacg ctcgcttcgg acacgccgat cggtaccgca ccgaggccga
     4081 gcgccagcgc gcgcgcaagc accgcggcgc cgatcggtcc gtcggtttcg ggcaggccgt
     4141 aatcgcgcat gatgaagccg ctgaccatca gcacgcgatc acccggtttc accctcgtcg
     4201 ccaggaggtc ggcggccgcg agcgtcagcg gctgcgttgc ctgcctgcgc gacgcctcgt
     4261 agagcctgtc gagcggaatg cctttgagga tcgtccagtt cgacatcggt gccgtgatca
     4321 ggtggtcgat cgcctcggcg atccgtgcaa gctcgatggt catggttcaa cgcgttcccg
     4381 tggcgacgac gcctctttca gcgtgacgat cgcgtccatt gtgatcgccc gcatggcttg
     4441 cgcgttgcgc atcgcgcaac tcaacggcga agcgtccggt atgctgacgc tcgatgcgcg
     4501 gcggttttcg ttgcgcgcta cgcaacatgc gatgacacgc acgcgagtgg acggcggcag
     4561 gcatgcgggc aacggcgcct cgcgggcgcc agggacgagc ccccggacgc cgcacgtgct
     4621 ggccgtcagg cacgccgtcg aggtgcttcg ctgccttgcg agcgatacgc cgcttctcgg
     4681 cgtcagcgag atcgcaagcg tggtcggcat gcacaagagc tcggtatcgc ggatggtcgc
     4741 aacgctcgag gaggacggcc tcgtcgagcg cgaccccgca tcgaagcgca ttcgcctcgg
     4801 gccgggcctg ttgagcctcg ccgcgccgtt gctcgcaagc gtgcgcgtca tcgagaccgc
     4861 gcagccgcag ctcgtcgagc tcgcgcagcg atgcggcgag acgatcagca tcagcgtctg
     4921 ggacggagcg ggcgcagtca acctggcgca ggtgctgggc ggcaaggcgg tcaagcacta
     4981 tgcggcgccg ggcagccgca accccgcgca tgccagcgca tcgggcaagc ttctgctcgc
     5041 cttcgcctcc gccgacaccg ccgagcgcat cctgcgccgc gagcttcggc gcttcacgcc
     5101 gcgcaccctc tgcacacgca gcgcgctgct cgcggaaatc gacacgatcc gccgccaagg
     5161 gtacgcggtc aacgagggcg agctttcgct cgacgtcggt ggcctcgcag cggcaattcg
     5221 caatgacaac gcgaacgtgg tggccgccat caccgcaacg gtgccgatgt accgattcgc
     5281 caccacgaac cggcgccagc tggtggagct cgtccgcgac gcggccgatc gcgtgtcggc
     5341 gcgcctcggc tatgcgcggg ccgaccatca cgcaaaccgc tgacccgcgg ttcgcgcgtc
     5401 ggcagccgca aacgatcggt cccgacccat ggccaggatc acccttctcc gagccaccga
     5461 ttacgctcgg cagcgatgga agaacggcgg cggcacgacg accgagctcg cacgcaacgg
     5521 caacgccgac cgctggctct ggcgcctgag cgtcgccgac gtcgagcgct cgggcccctt
     5581 ctcggatttc acggggtacc gccgcctcat cgtgctgctc gacggtcgcg gcatggcatt
     5641 gtcgttcgat gctgccgcgc cggtcgtgct cgacgagcgc tatcggccgt tcgcgttcga
     5701 cggcggcgcg cgcacgcatt gcgacctgct cgatggtccg atccgcgaca tgaacctgat
     5761 cttcgacgcc acgcgcatcg acgcctcgct cgacgtttgc acgctcgcac agggagcgtc
     5821 gcttcgcgtc gccgccagcg aatgtgcgtt gctgcacgca atcggaggac gtttcgacgt
     5881 gacgatcgga gacgacagcg ccgcgctgtc gccgggcgac acgctgcagg tcggcgagcg
     5941 tgaagcgatc ccgatcacgg cgacggcgct cgacgcgcat gccgttctcg ctcaggtcgc
     6001 gatcggctat cgaccgtgcc cgtcgtgccg atcgccaggc gtcggcgtcg acgcgacgcg
     6061 atgctagagt ggccgcaccg cccagcttgc gagagcccca tgaacgactc gtccgactcc
     6121 cgccacgccg cgcgcagcat ccgcgcgccg cgcggtgccg aactcacgtg cgccaactgg
     6181 ccgatcgagg cggcgtaccg gatgatccag aacaacctcg accgcgaagt cgccgagaac
     6241 cccgatgcgc tcgtcgtcta tggcggcatc ggcaaggcgg cgcgcaactg ggcgtgcttt
     6301 gacgcgatcc tcgccagcct gcgccggttg aagcccgacg aatcgctgct gatccagagc
     6361 ggcaagccgg tcggtatctt ccgcacgcat gccgacgcgc cgcgcgtgct gatcgcgaac
     6421 tccaatctgg tcccgaagtg ggcgacatgg gagcatttcg acgagctcga ccgcaagggc
     6481 ctgatgatgt tcggacagat gacggccggc tcgtggatct acatcggcag ccagggcatc
     6541 gtccagggca cgtacgaaac gttcgtcgag gcgggccggc ggcactacgg cggcgatctg
     6601 cgcggccgat ggattctcac cgcgggcctc ggcggcatgg gtggcgcaca gccgctcgcg
     6661 gcaacgttcg ccggtgcctg ctcgctcaac gtcgaatgcc agcaaagccg catcgacgcg
     6721 cgcttgcgca cgcgctatct cgacgagcag gccgccgatc tcgacgacgc gctggcgcgc
     6781 gttacccgct acacgtcgca aggccgggcc gcgtcgatcg gcctgctcgg caatgcggcg
     6841 gaactgctgc cggaaatcgt gcgccgcggc atcacgcccg atctgctgac cgaccagacg
     6901 tcagcgcacg atctcgtgaa cggttatctg ccgcccgggt ggagcgtcga gcggtggaag
     6961 gcggcgcagg ccgatccgct tcagcatgcg gcattgcgcg aggcggccgc gcgcggctgc
     7021 gcgacccacg tgcgcgcgat gctttcgctc caggcggtgg gtgtcccgac gttcgattac
     7081 ggcaacaaca tccgccaggt cgcactcgac cagggtgtcg cgaacgcgtt cgactttccc
     7141 ggcttcgtgc ccgcgtacat ccgtccgctc ttctgccgcg gcaagggccc tttccgctgg
     7201 gtcgcgcttt cgggcgatcc cgaggacatc cgcaagaccg acgcgaagct gaagctgctc
     7261 tttgcgcagg acgcgcatct tcaccgctgg ctcgacatgg cgggtgagcg catcgcgttc
     7321 cagggactgc cggcacgcat ctgctggctc ggcctcggcg agcggcatcg cgccgggctc
     7381 gcgttcaacg agatggtgcg caccggtgaa ttgaaggcgc cgatcgtcat cgggcgcgat
     7441 catctcgaca gcgggtcggt cgcttctccc aaccgggaaa cggaagcgat gcgcgacggg
     7501 tccgacgcgg tttccgactg gccgctgctg aacgcgttgc tcaacaccgc aagcggcgcg
     7561 acgtgggtga gcttccacca cggcgggggt gtcggaatgg gctattcgca gcacgccggc
     7621 gtcgtgatcg tgtgcgatgg caccgatgcg gccgcgcagc gcatcgagcg cgtgctgtgg
     7681 aacgatcctg gcagcggcgt gatgcgtcac gcggatgcgg gctacgacga tgcgatcgct
     7741 tgcgcgcgcg aaagcggcct cgacctgccg atgatcggcg cgtaaaaaag gcccgcgagc
     7801 gcgggccttt caatgatgcc ggaacgttct agttgcgatt cgcaaccgcg gcaagcttgg
     7861 cggtcggcgc aggcgtcgtc gccttgtcgc tcgcctgctt cgcttgcgtg gcgggggccg
     7921 ctttcgccac gacgggcgcg ggcgccttgc ttgcggcggg cggtgtggca agcccgattt
     7981 gcgcagcagg cgtcgatgat gccatcgaag cgtcgttgta ttcgcatgcc gagccgatcg
     8041 ctggagcaag accgaacagg gcgagcgccg ttccgacgag gattcgcttc atggatcacc
     8101 tcctttcgca gttgaccgaa ccacgaattt gcgcgcgccg tcccggattg tcaaccggat
     8161 accggcacag ggccggctgc gttgcaggcg cgggatcgca tgaccgagcg aagcgcggcc
     8221 gctatgtcaa taccgcggac gcggcggggt gctgacgcgc gtctcgagct gcctgaaagt
     8281 gatgcggccc ttcgacaggt cgtacgggga aatctcgagg gcaaccttgt cgcccgcgat
     8341 gatgcggatg cgatgcttgc gcatctttcc tgcgctgtaa gcgacgaggc tgtgaccgtt
     8401 gtcgagcgtg acgcgatagc gcgaatcggg cagcacctcg gccaccacgc cgttcatctg
     8461 gagaacttct tccttggcca atgggatcct ttattcgaaa ttgagaaagc aaaagcccgg
     8521 ctacgccggg cttcgatcgg gtgaggaggc gcggcaatca ccgcgcctcg cttccgttca
     8581 gtccaccgag cgaatgttcg ccgcctgcag acccttgggt ccggtcgtta cgtcgaaact
     8641 cacgcgctgg ccttccttga gcgtcttgaa gccctgcccc tggattgcgg aaaaatgggc
     8701 aaagagatcc ttgccgccat cctcgggcgt gacgaagccg aagcccttgg aatcgttgaa
     8761 ccacttcacg gtacctgttg ccatgtcttg actatcctga aaaaaatatg aaaggggaat
     8821 tgcccctgct ggggcgacga tcaagacggg cgggtgatga acagaagtac cgcagattcg
     8881 cggataccgg accagcaaca gcactacttg aaaatcgctg ttgtcaagta tggggcctcc
     8941 gcccggcaaa tacaagggtg gcgatgcgaa tccaggagat aatgggattg cgcgatcggg
     9001 tcgacacgtg ctccagggag gggcgatgct tgtccgaggc acattcctgc tgttgcttgg
     9061 cacgctcgcg ctcgccgcga acggcgcaaa cgccgcgccg ggcggctttc catcccggcc
     9121 gctgacgctg gtcgtcgggt tcgcgcccgg cggcggcatc gacatccagg cgcggctgct
     9181 cgcgaagggc ctcgagcgct acttcggcca gccggtgatc gtcgagaacc gcccgggcgc
     9241 cggatcgagg atcgccagcg agcacgtggc cagagcgtcc cccgacggtt acacgctgct
     9301 cgtgacggcc gcggcagccg ccatcgacat ggccttgagc gccaatgcgg gtgtcgacaa
     9361 gctgcacgat ttcgcacccg tttccactat atcgtcgtcg ccgctgattc tcgtcgtgaa
     9421 tccgtccgtg cccgtggcgg acgtgcaggc actggtcagg cgcgcccggt cgctgccggg
     9481 catgctcaat tattcgtctt ccggcgccgg caccaccggg cacctttacg gcgagctttt
     9541 caagcttcgc acccgcaccg acgtcgtcca tgtcccgtac aagggcagcg cgcccgcgct
     9601 tgccagcctg ctcgccggcg acgtcgagat gacctttgcc agctcggcgt cggtgatgca
     9661 attcgtgaag gcgggccgcg cgcgagcgct ggccaccacc ggcgcacgac gctcgcctct
     9721 gctgcccgat gtgccgacaa tcgaggaagc gggcgtcgcg aacgcgcaag ccgatatctg
     9781 gtatggggtg ctggcccccg ccgcaacgcc gcccgaagtg atcgaggcgt tggcgaaggc
     9841 aacgctgtcg gtgacgacgt ccgctgaatt cagccaacgc atgctcgggc tcggcgtcga
     9901 gccgagcgcg aacactcccg gcgcattccg cgtcctcttg cagaaagaag tggcgcggtg
     9961 ggccgaggtc gcgaaggcgg cgcacattca ggccgattga cgatggcatc gcagcccgac
    10021 acgcgctcgg tgggcgcgcg cctcgccgaa gtcgcgtcgc gcgtgcccga tcatccggcg
    10081 atcgtcgagc gcgatgcatg catgacgtac gccgcgctcg attcgctggc gacggcgatc
    10141 gcgtggcgca tcgtggcgac tcgcgaaagc cgtccgcgcg ccgtgtgcct gttgttcgaa
    10201 cgcaggctcg cgacgatcgc agcgctcttc ggcgcggcaa gaagcggacg cccctatgtt
    10261 ccgctcgatg ccggcgatcc cgaagcgcga ttgcggttca ttctcggcga ctgccaaccg
    10321 atcgcgctgc tgaccgagac ggcgctcgag ggccgcgcgc gcgcaatcgc tccaggtgga
    10381 tgcgaggtca tcgccatcga cgccctcgag ccagggggcg atgcggatgc gctccctgcc
    10441 gtcgcgtccg atgcaccggt ctaccttcac tacacgtcgg gctcgaccgg gcagccgaag
    10501 ggaatcagcc agaatcatcg caacgtgctc ttcctcgccg atgcctatgc gaaggcgttg
    10561 cgcatcggcg ccgccgaccg gctttcgctc ctctacacga tgagcttcgc cgcggcgaac
    10621 aatgccgtct ttcgcgcgct gcttcacggt gccaccctct gcgcctacga cctgcgccgc
    10681 ggtggcattg cggcgctgcc cgactggctc gatcgccagc gcatcacggt gctgcatctc
    10741 gtccccacgg catttcgcga actggcgacg cgtctcgcca ccgaccggct gctcccgcac
    10801 cttcgcgcga tccaccttgg cggcgagctg gtgttcgcga gcgacgtcga gctgttccga
    10861 aggcatacgc tcgaccactg catcctggtc aatcagctcg cgtcgaccga ggccgggccc
    10921 gtggcgcagg gaatcgtcga tcactggacc ccggtggcgc cgggcaccgt cgttcccgtc
    10981 ggccgctgca tcgacggcgt gcgcgtggaa attcgccgcg acgatggcgc gctcgccggc
    11041 atcggcgagg tgggcgagct cgtcgtgtgc agccgatacg tgagccccgg gtactggcgt
    11101 cgtcccgagc tcgacgccgc ggcgttctcg cccgatcccg cggatgcgga agcgcggcag
    11161 tttcgaagcg gcgatctcgg acacgtcgac gccgcgggca acctgcactt cctcgcgcgg
    11221 aagggcaacc gcgtgaagat tcgcggacat tcggtggacc tgacggagat cgagacggca
    11281 cttgccacgt gtcccggcgt tgcgacggcg gcggtcactg caatcgacga cgcaccgccg
    11341 gcccccgtgc gcttgctcgc cggtatcgtc gctgccgatg gcgcggtgcg cgacccgcag
    11401 atcgtccggc gtcaccttgc aacccgactg ccgatgtaca tgctgcccgc gcagatcgtg
    11461 tacgtcgacg cgcttccgtt caccgcaagc ggcaaggtcg accgtctcgc gctggcgcag
    11521 ataccggcgg cgccggcacc gcctgcgcgt gacatcgaag cgcccgagga cgacgtcgaa
    11581 cgcaaggtcg ccgaggtctt cgaggatttg ctcgaggtcg cgcacgcggg ccgcgacgat
    11641 gatttctttc tcctcggtgg cgattcgctc gcgagcgccg agcttcagtt gcgcctcacc
    11701 gaggcgttcg gtgtgcctgt ccgcaacttt cagcgtgacg cgacggtcgc ggcgatcgct
    11761 gccggtatcc gggacgcggc ggccgaacgc acgcaatcgc cgcaacgcct cccggtgctc
    11821 atgccgctgt ggcagaacgg ccgcgagccg ccgctgtttc tcgtgcacgg gcggcacggc
    11881 caggcattcg tgagcccgca cttcatgcgc ctgctcggcg acgagcaacc ggtgtgggta
    11941 ttccaggccc gcgggctcga cggcgtttcc gagccccatg cgagcgtcga ggcgatggcc
    12001 gccgagtatc tcgccgagat gcggcggcag cggccgcagg gtccgtactt cctcgggtcg
    12061 ctctgcgcgg gcgtgtatat cgttgccgcg atggcgcgcg cactgcgcga cgcgggggaa
    12121 cgggtgcttc cgctgctgct tctcgatccg ccgaacagcg ttctccaacc gggctattcg
    12181 cagctcacgc aggcgcagtt cgtcgacaag atgaaggcgc ggcgcgcgaa gggaaccacg
    12241 gcggggccca tggagaaccc ggcgtacatc gatgcgctgt tgcgcacggc gatggcgttc
    12301 gagcatgcga tcgcgaagca ccggccgctg ccgtacgacg gcccggtgta cgtgctgtcg
    12361 agctaccagc gcatgcgcgg cggcgattcc ttcgatctgc ggcgcatctt caccggccgc
    12421 tacaagcgct acgaggttgg aaccacgcac gcgcagtcgc tcgacccgcg aaacccggtg
    12481 ttcgcgagcg cgctgcggcg ttgcgtcgag ctgatacgcg acgcggcccg cgacaggccc
    12541 gcgatcgcct accgggtcca gggcggcgtg tcgtcggcgg aaatgccgta acgttgcagc
    12601 gcgctggcca cggttgccgc cggcaccgtg ctgcgcttcg ccagcgcgtg cagcgccgcg
    12661 acggcgatgt gatgccgatc gatctcgaaa tggccgcgta acgcgctgcg cgtgtcgctg
    12721 cgtccgaatc cgtcggttcc gagcgccacg aacggcgcat cgacgtacga cgcgatcagc
    12781 tgcggccacg cacgaacgta gtcggtggcc gcgacgatgg gcgcgctccc ggcaaggcac
    12841 tgcgcggcat ggcccgattt cgcgggcgca gtcggatgca gccggttcca gcgctcgacg
    12901 tcgcgcgcgt cgcgcgcaag ctcgctgaag ctcgtcacgc tccacacttc gctcgccacc
    12961 tgccaatcgt cgcgcagcag atcggcggcg gcgatcacct cgcgcaggat cgcgcccgac
    13021 ccgagcaagc gtacctgcgc cgcaccgctc gcggccgcga aacggtacat gccgcgaacg
    13081 atgtcggacg ccgcacccgg cgcaagcgac ggctgcgcgt aattctcgtt catcaccgtg
    13141 atgtagtaga agacgtcgtc ctggcgctcc atcatctgcc gcgcggcatg atcgacgatc
    13201 accgcgagct cgcacgcgaa tgccgggtcg tatgcgcggc aattgggaac ggtggccgcg
    13261 acgacatggc tgctgccgtc ctgatgctgc agcccctcgc caccgagcgt cgtgcggccc
    13321 gcggtgccgc cgagcaggaa cccgcgcgca cgctggtcgg ccgccgccca gatgagatcg
    13381 ccgacgcgct ggaagccgaa catcgagtag tagatataga acggcagcat cgcgacgccg
    13441 tgcacgctgt acgcggtggc tgccgcgacc cacgacgaca gcgcgccggc ttcggtgatc
    13501 ccctcctcga ggagctggcc gtcctttgct tcgcgatagc gcagcatcga cgcggcgtct
    13561 tccggctcgt acagctgacc ggatggcgag tagatgccga tctggcggaa gaggttggcc
    13621 atcccgaacg tgcgcgcctc gtcggcgacg atcggcacga tgcgcggacc gagcgcgcga
    13681 tccttcaaca ggttcgtgat cagccgaacc atcgccgtcg tcgtcgacat ctcgtgtccg
    13741 gcggcttcga gcgcgaagcg cgcgtaagcg tcgatcggcg gaatggggac ctgcgccgcg
    13801 tcgcggtgtc gcagcggaac ggggccgccc agtgccgcgc gccgatcgcg cagatacgca
    13861 agctcggggc cgtcggcggc gagcggatga aagcgcattt cgagcaggtc gtcgtcggaa
    13921 agcgtgagcc ggaaacgatc gcgaaaggcc ttcagcgcat cgaggtcgag ctttttctgc
    13981 tggtgcgacg tcatccgcga ctcgcccgcg ccgcccatgc cgtaaccctt cttagttttt
    14041 gcgagaacga ccgtcggctg tcccgggtgc gcctttgccg cgtgcagcgc cgcgtacagc
    14101 ttgcgcatat cgtgaccgcc gcgccgcaga gcatcgatct cggcgagcgt catgtgcgcg
    14161 accagcgcac gcaactccgg atcgagtccg aagaagtggc tctggttgta cgcgccatcg
    14221 ttcgcgccca gcgtctggta ctgcccgtcg accgtcgcgg cgaactggcg cagcagcgcg
    14281 tgatgcgtgt cgcgcgcgaa aagcgcgtcc cagtcggagc cccacagcac cttgatcacg
    14341 ttccagccgg cgccgatgaa tagcgactcg agctcctgga tgatctgccc gttgccgcgc
    14401 accggtccgt cgagccgctg caggttgcaa ttgatgatga acgtcaggtt gtcgagccgt
    14461 tcgcgcgcgg cgagcgtcag cgctgcgacc gactcgggct cgtccatctc gccgtccccg
    14521 aacacgcccc acacgtgccg atcgcccatc gtcgcgatgc cgcgatgctc gaggtagcgc
    14581 atgaagcgcg cgtggtggat ggcgctgatc gggccgatgc ccatcgatcc ggtggggaac
    14641 tgccagaagc cgggcatcag ccacggatgc ggatacgacg acagcccgtt gccgcccact
    14701 tcctgccggt agttcgcgag ctgcccctcg gagaggcgcc cctcgagaaa tgcgcgcgcg
    14761 tacacacccg gcgacgaatg cggctggaag aagacgagat cggcgccatg cggcgcgccg
    14821 gcgccgcgaa agaagtggtt gaagccaacc tcgaagatct ccgctgccga ggcatagctc
    14881 gcgatgtgac cgccaagctc gccatacgcg cggttcgcgc gcagcaccat cgcgagcgcg
    14941 ttccagcgga cgatcgccga gatgcgctcc tcgagcgcga ggtcgcccgg gtaggcgccc
    15001 tgctcctcga gcgggatcgt gttctggtac gccgagtacg tcggtacgtg ggtaacgatg
    15061 cgcaggtgct gcgcctgctc ctcgagcttt ttcagcagga acaggccgcg ctccctgccg
    15121 ccggtgcgca ccgtcgcctc gagcgcgtcg agccattccc gcgtctcctg cgggtcggca
    15181 tcgggttgga cgtctcgcat gtccatgggg ctcaccgtaa tgtggatggg ttctctgccc
    15241 ttgtccgggc gtgattagcc ggaccggggc aggaattata gtcc
//
LOCUS       DASSVX010000268         4449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_211871, whole genome shotgun sequence.
ACCESSION   DASSVX010000268 DASSVX010000000
VERSION     DASSVX010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211871"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..1361)
                     /locus_tag="VFO53_11475"
     CDS             complement(249..1361)
                     /locus_tag="VFO53_11475"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HET8876941.1"
                     /translation="MTSSLPNHAGRLAMQLALLAALALLIAFPAYAGDKIAPDAKANL
                     PAPAEAKANPPAQAADGSGAAGKIDPDKLFGAIVKVSTHSVPDARSADSLGTAREGTG
                     VVIGKDGLVLTIGYLLVEADKVEVTDSKGRVSPATIVANDQTSGFGLLRTLVPIDATP
                     IPLGDSGKSTEREPVLIASAGGEGASFAWIVSKRPFTGNWEYRLDYALFTSPPTTDWS
                     GAALIDGDGKLLGIGSLIVREATDGDTKLPGNVFVPIDLLKPILADLVRQGHRAGPAR
                     PWLGVSADELQGHLFVTKVLPDSPAERAGLVVGDIILGVGTDPVRNQDEFYQHVWARR
                     QAGDEVPLKVLKGVDVKDIAVRSMDHLAYYRPQSTI"
     gene            1585..3315
                     /gene="araD"
                     /locus_tag="VFO53_11480"
     CDS             1585..3315
                     /gene="araD"
                     /locus_tag="VFO53_11480"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HET8876942.1"
                     /translation="MSPSRKRPEDLRSHRWYGTSDLRAFGHRSRTAQMGYARADYLGK
                     PVIAIINTWSDINPCHTHFRQRAEEVKRGVWQAGGFPVEMPALTLSEPFQKPTTMLYR
                     NLLAMETEELLRSYPADGAVLMGGCDKTTPALMMGAISMNLPAIFLPAGPMLRGDWRG
                     QFLGSGSDTWKYWAEKRAGKLSDEAWEEIENGIARSPGHCMTMGTASTMTSAVEALGM
                     TLAGAASIPAADSRHARMATATGRRIVDMVWEDVKPRDILSADSFDNAITTVLALGGS
                     TNAVVHLVAMARRAGIALSIDRFDALSRRTPLVANVRPAGKYLMEDFYYAGGLRALMA
                     CIRDLLALDARTVEGATLREAIGEAEVFNDDVILPRERALVASGSLAVLRGNLAPDGA
                     VIKPAAAEARLLRHRGPAIVFADYNDMAARIDDPALPVTADSVIVLKHAGPLGAPGMP
                     EWGQLPIPKKLLEQGVRDMVRISDARMSGTSYGACVLHVAPESYVGGPLALVHDGDTI
                     VLDVPGRRLHLDVDDAELARRRAAWRRPERHFERGFGALYQQHISQANDGCDFDFLEG
                     TSPTPDPEIH"
     gene            3358..4239
                     /locus_tag="VFO53_11485"
     CDS             3358..4239
                     /locus_tag="VFO53_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8876943.1"
                     /translation="MSGSPLFPGFTTHRIGTASTQIHCVVGGNGPAVLLLHGYPQTHA
                     MWHRVAPALARHCTVVCADLRGYGDSGKPPSNGTHATYSKRAMAQDMVELMRNLGHAQ
                     FRLVGHDRGGRVAHRLCLDHPGAVTQLVVLDISPTRTMYERTDRAFATAYYHWFFLIQ
                     PFDLPERMIGADPVYYLRRKLGGWGTGLEHFDLCALAEYERCFSNPETIHASCEDYRA
                     AASIDLEHDAAEQKITCPMLVLWGERGVVNRLFDPLADWRAVATNVRGRALQAGHYLA
                     EEAPDETLRELMQFFAM"
     gene            4378..4449
                     /locus_tag="VFO53_11490"
     tRNA            4378..4449
                     /locus_tag="VFO53_11490"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4411..4413,aa:Phe,seq:gaa)
BASE COUNT          731 a         1469 c         1496 g          753 t
ORIGIN      
        1 aggtcgtcca taccgcggcc gacccgccga accacggcag gatctgcttc gcgatgatgg
       61 gctggaccag aaagagaagg aacgcactcg cgaagatcgt cagtgcgaag aggggcatga
      121 gcctggcgtc ggaaggccgg catcgtcgtc ttcgcacgag cgaagacgct gcgcggtgtc
      181 gcgcattcta gcggccggcg cgcgaagccg agggattcgg cgcctgcgat gcagcgtcgc
      241 gtcccgactc agatcgtcga ctgcggccgg tagtacgcga gatggtccat cgagcgaacc
      301 gcgatgtcct tcacgtcgac gcctttcagc accttgagcg gtacttcgtc gccggcttgc
      361 cgccgcgccc atacatgctg atagaactca tcctggttgc gcacgggatc cgtgccgacg
      421 ccgagaatga tgtcgcccac gacgagaccc gcccgctcgg cgggactgtc gggtaacacc
      481 ttcgtcacga agaggtggcc ctgcagttcg tccgcgctga caccgagcca cggccgcgcg
      541 ggtcctgcgc ggtggccctg ccgcacgaga tcggcaagga tcggcttcaa aagatcgatc
      601 ggcacgaaca cgttcccggg cagcttcgta tcgccgtcgg tggcttcgcg cacgatcagc
      661 gagccgatgc cgagcagctt gccatcgccg tcgatcagcg ccgcgccgct ccagtcggtg
      721 gtgggcgggc tcgtgaacaa cgcgtagtcg aggcgatatt cccagttgcc ggtgaacgga
      781 cgcttcgaca cgatccacgc gaacgacgcg ccttcgccgc cggcgctggc aatcagcacc
      841 ggctcgcgtt cggtggactt gcccgagtcg ccgagaggga tcggtgtcgc gtcgatcggc
      901 accagcgtgc gcaacaggcc gaagccgctg gtctgatcgt tcgcgacgat cgtggccgga
      961 gatacgcggc ccttcgaatc ggtgacctcg accttgtcgg cctcgacgag cagatagccg
     1021 atcgtcagca ccaggccgtc cttgccgatg accacacccg ttccctcgcg cgcggtgccg
     1081 agcgaatcgg cgctgcgcgc atcgggcacc gagtgggtcg acaccttcac gatggcgccg
     1141 aacagcttgt cggggtcgat cttgccggcg gcgccgctgc cgtccgcggc ttgtgcgggc
     1201 gggttggctt ttgcctcggc tggcgcaggc aggttggctt tcgcatcggg agcgatcttg
     1261 tccccggcgt acgccggaaa ggcgatcagc agcgcgagcg ccgcgagcaa tgcgagctgc
     1321 atcgccagac ggcccgcgtg attgggtaga gacgatgtca tgacaacctc ccgatcctgt
     1381 ttccaacaca cggggcacga atccatcttc gcgcgcggcg ctatactctg caaaaaatgc
     1441 agcgtccggc aggagaattt cccgcgacgt cggacggctc ccgaccgcgc tttcgtgcgc
     1501 actgccgtga acggcgccga tgcgcggcaa atacgtgccg gtcaccgctt ttcgaagcaa
     1561 gcggcgggcc catcgagatc gttcgtgtcg ccatcgcgca aacgccccga agaccttcga
     1621 tcgcaccgct ggtacggaac gtccgacctg cgcgcgttcg gccaccgctc ccgcaccgcg
     1681 cagatgggtt acgcgcgcgc cgattatctc ggcaagcccg ttatcgcgat catcaacacg
     1741 tggagcgaca tcaacccgtg tcacacgcac tttcgccagc gcgccgagga ggtgaagcgc
     1801 ggcgtgtggc aagccggcgg atttccggtg gagatgcccg cgctcacgct gtcggagccg
     1861 ttccagaagc cgacgacgat gctctaccgg aacctgctcg cgatggaaac ggaggagctg
     1921 ctgcgctcgt atccggccga cggcgccgtg ctgatgggcg gatgcgacaa gaccaccccc
     1981 gcgctcatga tgggcgccat cagcatgaac ctgccggcga tctttctgcc ggccggaccg
     2041 atgctgcgcg gcgattggcg cggccagttc ctcggctcgg gatcggatac ctggaaatac
     2101 tgggcggaga aacgcgcagg caagctgtcc gacgaggcgt gggaggaaat cgagaacggc
     2161 attgcgcgct cgcccggcca ttgcatgacg atgggtacgg cgtcgacgat gacgtcggcc
     2221 gtcgaggcgc tcggcatgac gctcgccggt gcagcgtcga ttcctgcggc cgattcgcgg
     2281 catgcgcgca tggcgaccgc caccggacgg cgcatcgtcg acatggtgtg ggaggacgtg
     2341 aagccgcgcg acatcctcag cgccgacagc ttcgacaatg cgatcaccac ggtgctggcg
     2401 ctcggcggat cgacgaatgc cgtcgtgcat ctcgtcgcga tggcacgccg cgccggcatt
     2461 gccttgtcga tcgaccgctt cgacgcgttg tcgcggcgca cgccgctcgt cgcgaacgtg
     2521 cgtccggcag gcaagtacct gatggaggat ttctattacg ccgggggact gcgcgcattg
     2581 atggcgtgca ttcgcgacct gctcgcgctc gatgcgcgca ccgtcgaggg cgcgacgctg
     2641 cgcgaggcaa tcggcgaggc ggaagtattc aacgatgatg tgatcctgcc gcgcgaacgc
     2701 gcactcgtcg cgtccggaag ccttgccgtc ctgcgcggca atctcgcgcc ggatggcgcc
     2761 gtgatcaagc ccgccgcggc cgaggcgcga ttgctgcggc accggggacc ggcgatcgtg
     2821 ttcgccgatt acaacgacat ggccgcgcgc atcgacgatc cggcgctgcc ggtgaccgcc
     2881 gactcggtca tcgtgctgaa gcacgcgggc ccgctcggcg cacccggcat gcccgaatgg
     2941 ggccagctcc cgatcccgaa aaagctgctc gagcagggcg tgcgtgacat ggtgcgcatc
     3001 tccgatgccc ggatgagcgg aacgagttat ggcgcatgcg tgctgcacgt ggcacccgaa
     3061 tcgtacgtcg gcggcccgct tgcgctcgtt cacgacggcg acacgatcgt gctcgacgtg
     3121 cccgggcgcc gcctgcacct cgacgtcgac gatgctgaat tggcgcgccg gcgcgccgca
     3181 tggcgccggc ccgagcgcca tttcgagcgc ggcttcggtg cgctctacca gcagcacatt
     3241 tcgcaggcga acgacggttg cgatttcgat tttctcgaag gcacgtcgcc cacacccgat
     3301 cccgagattc attaggatgg atcgtcgctc gccggtgtcg ttgcggctgc gcctgcgatg
     3361 agcgggtcgc cgctgttccc gggtttcacg acccatcgca tcggtaccgc atcgacgcaa
     3421 atccactgcg tggtcggcgg caatggaccg gcggtgttgc tgctgcacgg ctatccgcag
     3481 acgcacgcga tgtggcatcg ggtggcgccc gcgctggcgc ggcattgcac ggtcgtgtgc
     3541 gcggatctgc gtggctacgg cgactcgggc aagccgccgt ccaacggcac gcacgcgacg
     3601 tattcgaagc gcgcgatggc gcaggacatg gtcgagctga tgcgcaatct cggtcacgcg
     3661 cagtttcggc tcgtcggcca cgatcgtggc ggccgcgtcg cgcaccgatt gtgtctcgac
     3721 catcccggcg cggtgacgca gctcgtcgtt ctggatatct cgccgacgcg cacgatgtac
     3781 gagcgcaccg atcgggcgtt cgccacggcc tactaccact ggttcttcct gatccagccg
     3841 ttcgacctgc cggaacggat gatcggcgcc gatcccgtgt attacctgcg gcgcaagctc
     3901 ggcggctggg ggaccggcct cgagcatttc gacctgtgcg cgctcgccga atacgagcgc
     3961 tgtttcagca accccgaaac gattcatgcg agctgcgagg actatcgcgc cgccgcgtcg
     4021 atcgacctcg agcacgacgc ggcggaacag aagatcacct gcccgatgct ggttttatgg
     4081 ggcgagcgcg gggtcgtgaa ccggctgttc gatccgctcg ccgactggcg cgcggtcgcg
     4141 acgaacgtgc ggggccgcgc gctgcaggca ggtcactacc ttgccgaaga ggctccggac
     4201 gagaccctgc gcgaattgat gcagttcttc gcgatgtagc gcggcacgtc gcgcacccgc
     4261 acgtatcgag cgctcgacgc tcccggggtc gtccgcggag cgcggacgcg ctggtataat
     4321 tcgccgcttc ggccggaagc tttcgcggga acggaagctc cggccgctgc agttcatggc
     4381 caagtagctc agttggtaga gcagaggact gaaaatcctt gtgtcggtgg ttcaattccg
     4441 cccttggcc
//
LOCUS       DASSVX010000269         9730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_895169, whole genome shotgun sequence.
ACCESSION   DASSVX010000269 DASSVX010000000
VERSION     DASSVX010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895169"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VFO53_11495"
     CDS             complement(<1..824)
                     /locus_tag="VFO53_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876944.1"
                     /translation="MMIGKWVGRVLAAACIGAMGAGAGAAQAADDVSVQLDWLVRGNH
                     AMFFVARDKGYFSQAGINVTAIRKGTGSTDALKLVANGNADFGFADLPTLQVGRSQKL
                     PVVALAAVNQESPLAMISIKSKHPMSKPADMKGMNIGVHPSGSTYVFLKAFFAKNGLD
                     LAGFKQSTVSPPYENMLLLGRVDLVPGYIDAEVPELEAKAGGPGSLSILLGADYGYDA
                     YGSGLFTSEKMIADKPDVVQRFVKAYLQAFAFVVENPQQAVDIVVKANPEYKGKEST"
     gene            complement(896..1735)
                     /locus_tag="VFO53_11500"
     CDS             complement(896..1735)
                     /locus_tag="VFO53_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876945.1"
                     /translation="MTSMVNSETAHAPVNVEAGACAGSGTSMRATWARFMPPLALFVG
                     VVVAWEAVSRIFRLPEFVLPAPTAIVEVLISNQRALAYAAFVTAREVLWGFLLSAVIG
                     AAVALVIARFAAFGRALYPLVVLFQNVPKVALAPLFILWFGFDLAPKVVLIVVIAFFP
                     VAINMLVGLQTVDANLIALMRSVGASRNEILLRIQVPNSLPYLMAGLKVAVTFAVIGA
                     IVGEFAGAQAGLGYMIQFASTQMQTSLVFAALVEVSVLGMLFYYVIEWVERRYIDWMP
                     AAA"
     gene            complement(1732..2547)
                     /locus_tag="VFO53_11505"
     CDS             complement(1732..2547)
                     /locus_tag="VFO53_11505"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876946.1"
                     /translation="MSASPSSAPQAHILARGVCVDFTVAGRVNRVLHDVSLSVPKGGF
                     VSLIGPSGCGKSTLLKVLAGLLAPTAGNVSVAGVTPIEAVRQRRIGIVFQEATLLPWK
                     NALDNVALLMEVAHGGRSSDEVQQRAAEMLRLVGLEHAAVKRPAQLSGGMRQRVSIAR
                     ALALDPEVLMMDEPFGALDAITREEMSEFLLEIWERTKKTIILVTHSIDEAAFLSRKV
                     NVMAANPGRIIEAMPIDLPYPRNEDSYAAPEFARATARLRRLLKEGHRQALAA"
     gene            complement(2568..3164)
                     /locus_tag="VFO53_11510"
     CDS             complement(2568..3164)
                     /locus_tag="VFO53_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET8876947.1"
                     /translation="MIELLGRATSGNVQKVIFALEELGLPYERKDYGRQFENTTTPQY
                     AALNPMKKVPTLVDGDTVVWQSNTILRYLANKTGSTLYPADAAQRSQVERWMDWLLAE
                     LNPAYLAGFRDAKKAEGERAPDTATNLGNELALLEGALGKTAWVAGAQFSLADVALGP
                     IIKRCVGFPLGLPAMPKTQAWIAALQKRPAFQKATATG"
     gene            complement(3191..3589)
                     /locus_tag="VFO53_11515"
     CDS             complement(3191..3589)
                     /locus_tag="VFO53_11515"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET8876948.1"
                     /translation="MSTQSRTENPRIEVYGKKNPNLPFHPAVRAGDYVYVSGQVGKDA
                     DGNMIAGTIEVETRATLDAVKRVLADAGCELADVVKVTTYLEDARDFGRYNGVFKEYF
                     PEGRIARTTVEARAVIGTKIEIDCIAYKPL"
     gene            complement(3586..4716)
                     /locus_tag="VFO53_11520"
     CDS             complement(3586..4716)
                     /locus_tag="VFO53_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876949.1"
                     /translation="MARAANTRLVSHVDCPGGGQVWWDRDVLYVSHMRPPDGTSIYDV
                     ADPKHPKLIAKLDVPMGWHSHKVRVANGLMVVNYEKFRDGAPEFGGGLGIFDVSTPAR
                     PKLVNKWRVSGEGGGVHRYDFDGRYAYISPTADGYVGNIMMILDLANPEAPEEVGRWW
                     IPGQHVAGGEEYPWDDYVKPRCHHPLRHGDRLYVSYWHHGFFILDIADMTKPRLVSAV
                     NTGPADPHPTHTALRMPHRLKGRDIMLVADEDVAKLRPSAPAFAWVYDISFEKQPTPI
                     ATFQVPGLDTDGAPQPPMMGCHQPSERFHGTTVPFAWFARGLRLVDVSDPFAPREAGY
                     YEPDVPEGYAMPSSNDVTVDPSGLIYLIDRQRGLDIIESRVS"
     gene            complement(4721..5869)
                     /locus_tag="VFO53_11525"
     CDS             complement(4721..5869)
                     /locus_tag="VFO53_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8876950.1"
                     /translation="MGNRRELTLSGAAKEAALREMYADINARNMFPVWARASDVEHDE
                     IRQLMSGPKPVPFRWSYKDDIESLLHRSAELVTTEDTDRRSLVLVNPGLAPRRATIST
                     LYTAYRLNDPNEIMPPHRHTASAIRFGLTGTKNFTGVEGEDITFGPGDMVLTPRDTWH
                     NHGNVGDAPAINLSVLDYPLVENLNALAFDHDYKEGGVAKNKQSARFPTDYSARVYGT
                     GGLRPRFVDHFRGVGGSSPMFVYRYDAMRELLERHRDYAGNPHEGLLIEYIDPLTGGP
                     VYRTMTFFMQMVRNAETTLPIKQSASLLVAPFEGRGHALIDGKRFELDRFDTVAVPGG
                     HWVEYVNDGDEPLILFVASDEPALAAFGLLTRYGRTASGDVVNLLQRA"
     gene            6070..6723
                     /locus_tag="VFO53_11530"
     CDS             6070..6723
                     /locus_tag="VFO53_11530"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HET8876951.1"
                     /translation="MRNQAQPQAPDVATLARRLRDRLANGEALPAERALAEQYAVKRH
                     RVRQALALLREQGDLDSSRRTGVADGESLIRSTNPLEVIEVRLALEPALARLAAVRAT
                     PLEIARIMRAATTPSDMQRSTADLVFHKLVAAASGNGLAADLYALLRKVGADARVRVQ
                     SSAPACPNRLAQRDQEHHGVAEAIAARDPEGAEAAMRAHLYAVHRIILNRLMAANAA"
     gene            complement(6727..7671)
                     /locus_tag="VFO53_11535"
     CDS             complement(6727..7671)
                     /locus_tag="VFO53_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET8876952.1"
                     /translation="MTVDKISNVVIVGAGTMGPGIAATFARHGYATTLCDSDAKQIDK
                     ARVGIDLVFATLQGGGFATDAEVDAARSRLTLVQEPAGAIAAADFVLENVPERLELKR
                     AIFAELCATARDDAILASDTSGIPISTLQAGMRNPGRIIGMHWSNPPHLIPVIEVVKG
                     SETAASTVDSTVALVESLHMLPGIVERDVPGFVENRILYAIMREALHLLDEGVASAEA
                     IDRIVKWGIGYKLGVIGPLRLLDMAGLDIYTAVAGYLNKDLCNDPGISTTVASKVGDG
                     RLGLKTQAGLFDYTHEEIEQLARERGRLLVASKKALTS"
     gene            complement(7704..8537)
                     /locus_tag="VFO53_11540"
     CDS             complement(7704..8537)
                     /locus_tag="VFO53_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET8876953.1"
                     /translation="MMTTVEPSIARLDATEIAHYRQEGWVVPRFRLPPHEVARLREAL
                     DALIRANPGVRPEKLVSAHVDGDNGEGVRGDARFLALARDPRIVELVSDVIGDDIVLW
                     GCHVFCKPAGDGYETPWHQDGHYWPIRPLANCTVWVALEPSTIENGCLRVIPRSHLGR
                     TLHEHLHEERTDLTLNQRLAAGSFELRDAVDIELEPGQMSLHDVYMIHGAAPNRSSKR
                     RTGVALRYMPATSVFERNVRPAEGATGVPVDFAHRPLWLVRGVDRSGRNDFDVGHRRR
                     D"
     gene            complement(8647..9444)
                     /locus_tag="VFO53_11545"
     CDS             complement(8647..9444)
                     /locus_tag="VFO53_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET8876954.1"
                     /translation="MTDHKTGTREEWLAARRQLLEAEKTLTRRSDELARMRQALPWVR
                     IDKDYRFETSEGSASLADLFRGRSQLLVYHFMFGPDYTAGCPSCSAIADGFDGFVVHL
                     ANHDVTLCAVSRAPFARLQAYERRMGWTFPWASSFDSDFNYDFNATYTEEQQREGGIE
                     YNYRHEPAWQVRSDEGIASRMPDTPVAKMAAMSGTDAATYTRERPGMSAFALDDGVVY
                     HAYSSYARGLDALWGMYPWLDRAPRGRNETGIWWRRHDEYGDRPGTP"
     gene            complement(9441..>9730)
                     /locus_tag="VFO53_11550"
     CDS             complement(9441..>9730)
                     /locus_tag="VFO53_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876955.1"
                     /translation="QVDPQTGAILRTIESNRFVTGVTWVDGELWHGTWDGDESEIRRI
                     DPASGAVLERLEMPHGTGVSGLESDGTDLFYCGGGASGKVRAVRRPKGDRR"
BASE COUNT         1626 a         3246 c         3183 g         1675 t
ORIGIN      
        1 gtcgactcct tgcccttgta ttccggattg gccttcacga cgatgtccac cgcctgttgc
       61 ggattctcga cgacgaacgc gaacgcctgc agatacgcct tgacgaagcg ctgcacgacg
      121 tcgggcttgt cggcgatcat cttctccgat gtgaagagcc ccgagccgta cgcgtcgtag
      181 ccgtagtcgg cgccgagcag gatcgacagc gatccgggcc cgccggcctt cgcctcgagc
      241 tccggcactt cggcgtcgat gtaaccgggc acgaggtcga cgcggccaag gaggagcatg
      301 ttctcgtacg gcggcgaaac ggtgctctgc ttgaagccgg cgagatcgag gccgttcttg
      361 gcgaagaacg ccttgaggaa cacgtacgtc gagccgctcg gatgcacgcc gatgttcatg
      421 cccttcatgt cggcgggctt gctcatcggg tgcttcgact tgatcgagat catcgccagc
      481 gggctttcct ggttgacggc ggcgagcgcc accaccggaa gcttctgcga gcgaccgacc
      541 tgcagcgtcg gcaagtcggc gaagccgaaa tcggcgttgc cgttggccac gagcttcaac
      601 gcatcggtcg acccggtgcc cttgcgaatg gcggtgacgt tgatgcccgc ctgcgaaaag
      661 tagcccttgt cgcgcgcgac gaagaacatc gcgtggttgc cgcgcaccag ccagtcgagc
      721 tgaaccgaga catcgtccgc cgcctgtgcg gcgccagcac ccgcgcccat cgccccgatg
      781 caggcggctg caagcaccct ccccacccat ttgccgatca tcatgattaa ccgcccctct
      841 cgttgacaac gatgtgcatc gacgccgttc gccaagcttg cggcgccggc gtcgttcagg
      901 ccgccgccgg catccagtcg atgtagcgcc gctcgaccca ttcgatcacg taatagaaca
      961 gcatgccgag gaccgacacc tcgaccagtg cggcgaacac gagcgacgtc tgcatctgag
     1021 tggatgcaaa ctggatcatg tagccgagcc cggcctgcgc gcccgcgaac tcgccgacga
     1081 tggcgccgat caccgcgaag gtgaccgcga ccttgagccc ggccatcagg tagggcagcg
     1141 aattgggaac ctggatgcgc agcaggatct cgttgcggct cgctcccacg gagcgcatca
     1201 gcgcgatcag attcgcgtcg accgtctgca agccgaccag catgttgatg gcgacgggga
     1261 agaacgcgat cacgacgatc agcaccacct tgggtgccag gtcgaatccg aaccacagga
     1321 tgaagagtgg cgccagcgcc accttcggca cgttctggaa cagcacgacc agcggataca
     1381 gcgcgcgccc gaacgccgcg aagcgcgcga tcaccagcgc gacggcggcg ccgatgacgg
     1441 ccgaaagcag gaatccccac aacacttcgc gcgcagtcac gaatgcggcg taagcgagcg
     1501 cccgctgatt cgagatcagc acctcgacga ttgccgtggg tgccggcagc acgaactcgg
     1561 gcagcctgaa aatgcgcgac acggcttccc acgccacgac gacgccgacg aacagcgcca
     1621 gcggaggcat gaagcgcgcc caggtggcgc gcatgctggt gccggaaccg gcacaggcac
     1681 cggcttcgac gttcaccggt gcgtgcgcgg tttcgctgtt gaccatactt gtcatgccgc
     1741 caacgcctgg cgatgccctt ccttcaacag tcgccgcaag cgggccgtcg cacgcgcgaa
     1801 ttccggcgcc gcgtagctgt cctcgttgcg cggataaggc aggtcgatcg gcatcgcttc
     1861 gatgatgcga cccggattgg cggccatcac gttcactttg cgcgacagga acgccgcttc
     1921 gtcgatcgag tgcgtgacga ggatgatggt cttcttcgtg cgctcccaga tctcgagcag
     1981 gaactcgctc atctcctcgc gcgtgatcgc gtcgagcgca ccgaacggct cgtccatcat
     2041 cagcacttcg ggatcgagcg ccagcgcgcg cgcgatcgat acccgctgcc gcatgccgcc
     2101 cgacagttgc gccgggcgct tcaccgccgc atgctcgagg ccgacgagac gcaacatctc
     2161 ggcggcacgc tgctggactt cgtcgctgct gcgtccgccg tgcgccacct ccatcaacag
     2221 cgcgacgtta tcgagcgcgt tcttccacgg cagcagcgtc gcttcctgga acacgatgcc
     2281 gatgcgccgc tgccgcaccg cttcgatcgg cgtgacgccg gccaccgaca cgttgcccgc
     2341 cgtgggcgcc agcaatccgg cgagcacctt gagcagcgtg ctcttgccgc atcccgacgg
     2401 gccgatgagc gagacgaagc cgcccttcgg aaccgacagg ctgacgtcgt gcagcacgcg
     2461 attcacgcgt ccggcgacgg tgaaatcgac gcaaaccccg cgcgcgagga tatgcgcctg
     2521 cggcgcgctg ctcggcgatg cactcatggc gctgatggcg acgcggatca gccggtggcg
     2581 gttgccttct ggaacgccgg acgcttttgc agcgcagcga tccaggcttg cgtcttcggc
     2641 attgcgggga ggcccagcgg aaacccgacg cagcgcttga tgatcggacc gagtgccacg
     2701 tcggcgagcg agaactgcgc gccggcgacc cacgcggttt tgccgagcgc gccttcgagc
     2761 agggcgagct cgttgccgag attggtcgcc gtgtcgggag cgcgttcgcc ttccgccttc
     2821 ttcgcatcgc gaaagccggc gagatacgcg ggattgagct cggccagcaa ccagtccatc
     2881 cagcgctcga cctgcgagcg ttgcgccgca tcggccgggt acagtgtgct gcccgtcttg
     2941 ttcgcgagat agcgcaggat cgtgttcgat tgccacacga ccgtatcgcc gtcgacgagc
     3001 gtcggcacct tcttcatcgg gttcagcgcc gcgtattgcg gcgtggtcgt gttctcgaac
     3061 tggcggccgt aatccttgcg ctcgtacggc aggccgagct cctcgagtgc gaatatcacc
     3121 ttctgcacgt tgccggacgt cgcccggccc aatagttcga tcatcgctgg gtatccttga
     3181 atggttgaga tcagagcggc ttgtacgcga tgcagtcgat ttcgatcttc gtgccgatca
     3241 cggcgcgcgc ctcgacggtg gtacgcgcga tgcgcccctc gggaaaatac tccttgaaca
     3301 cgccgttgta gcgtccgaag tcgcgcgcgt cctcgaggta agtcgtcacc ttcacgacgt
     3361 cggcaagctc gcagcccgcg tcggcgagca cacgcttgac tgcgtcgagc gtggcgcgcg
     3421 tttcaacttc gatggtaccc gcgatcatgt tgccgtccgc atccttgccc acctggccgg
     3481 acacgtagac ataatccccg gcgcgcaccg cgggatgaaa cggcaggttc ggattcttct
     3541 tgccgtagac ctcgatgcgt ggattttccg tgcgcgattg cgtgctcatg acacgcggct
     3601 ttcgatgatg tcgaggccgc gctggcgatc gatcaggtag atgagtcccg acgggtcgac
     3661 ggtgacgtcg ttcgacgatg gcatggcgta accctcgggc acgtcgggtt cgtagtaccc
     3721 cgcctcgcgc ggtgcaaagg ggtcgctcac gtcgacgagc cgcaggccgc gcgcgaacca
     3781 tgcgaacggg accgtcgtgc cgtgaaagcg ttccgaaggc tggtggcagc ccatcatcgg
     3841 cggttgcggc gcgccgtcgg tatcgagccc cggcacctgg aacgtcgcga tgggcgtcgg
     3901 ttgcttctcg aagctgatgt cgtagaccca ggcgaaggcc ggcgccgatg gccgcagctt
     3961 ggcgacgtct tcgtcggcga cgagcatgat gtcgcgtccc ttcaacctgt gcggcatgcg
     4021 cagcgccgtg tgcgtcggat gcggatcggc cggaccggtg ttgactgcgg aaacgagcct
     4081 cggcttcgtc atgtcggcaa tgtcgaggat gaaaaagcca tggtgccagt acgaaacata
     4141 cagccggtca ccatggcgca gcggatgatg acagcgcggc ttgacgtaat cgtcccacgg
     4201 gtactcctcg ccgccggcga cgtgctgacc gggaatccac cagcggccca cttcctcggg
     4261 tgcttccgga ttggcaaggt cgaggatcat catgatgttg ccgacgtagc cgtccgcagt
     4321 cggcgagatg tacgcgtagc ggccgtcgaa gtcgtagcga tgcacgccgc cgccctcgcc
     4381 gctgacgcgc cacttgttga cgagcttcgg cctcgctggc gtcgacacgt cgaagatgcc
     4441 gagaccgccg ccgaactcgg gggcgccgtc gcggaatttt tcgtagttga ccaccatcag
     4501 gccgttcgcg acgcgcacct tgtgcgaatg ccagcccatc ggaacgtcga gctttgcaat
     4561 gagcttcgga tgcttcggat cggcgacgtc gtagatcgac gtgccgtcgg gcggccgcat
     4621 atgcgacacg taaagaacgt cccggtccca ccacacctgc ccgccgccgg gacagtcgac
     4681 gtggctgacg agcctcgtgt tcgccgcgcg cgccatcggg ttacgcgcgt tgcagcaggt
     4741 tgaccacgtc gccggatgcc gtgcggccgt agcgcgtgag cagtccgaac gccgcgagcg
     4801 ccggctcgtc gctggcgacg aacaggatca gcggttcgtc gccgtcgttc acgtactcga
     4861 cccaatggcc gccgggaacg gccaccgtgt cgaaacggtc gagctcgaag cgcttgccat
     4921 cgatcagcgc gtggccgcga ccttcgaacg gcgcgacgag gaggctggcc gactgcttga
     4981 tcggcagcgt cgtttcggcg ttgcgcacca tctgcatgaa gaacgtcatc gtcctgtaca
     5041 cgggcccgcc ggtcagcgga tcgatgtact cgatcagcag tccctcgtgc ggattcccgg
     5101 cgtagtcgcg atggcgctcg agcagttcgc gcattgcgtc atagcgatag acgaacatcg
     5161 gcgacgagcc gccgacgccg cggaagtgat cgacgaaccg cggcctgagt ccgccggtgc
     5221 cgtagacgcg cgccgagtag tcggtgggaa agcgcgccga ctgcttgttc ttcgcgacgc
     5281 cgccttcctt gtaatcatga tcgaaagcga gcgcgttgag gttctcgacg agcgggtagt
     5341 cgagcaccga aagattgatc gccggcgcat cgccgacatt gccatggttg tgccaggtgt
     5401 cgcgcggcgt cagcaccatg tcgccgggac cgaacgtgat gtcctcgccc tcgacgccgg
     5461 tgaagttctt cgtgccggtc aggccgaagc ggatggcgct ggccgtgtgg cggtgcggcg
     5521 gcatgatctc gttgggatcg ttcagccggt atgccgtgta aagggtgctg atcgtcgcgc
     5581 ggcgcggcgc gagcccggga ttgacgagca ccagcgagcg gcggtcggtg tcctcggtgg
     5641 tgacgagctc ggcggagcgg tgcagcaacg actcgatgtc gtccttgtag ctccagcgga
     5701 acggaaccgg cttcggcccg ctcatcaatt gccggatctc gtcgtgctcg acgtcggatg
     5761 cgcgcgccca gaccggaaac atgttgcgcg cgttgatgtc ggcgtacatc tcgcgcagcg
     5821 ccgcctcctt cgccgctccc gaaagcgtca gttcacgacg attgcccatg atgcgtccct
     5881 cgcggagtcg acgcggcgat agtgccaagc cgggagcgga ctgtcaacca gcggttaacc
     5941 gcagcgactt tggttgcacg aacgatgcga caaggcagcc gatcatcgac gcccgaagct
     6001 cccgacgaat cgctcttgtg gcccgcatga aacaccgtta gcatccgcgt aaccgacgga
     6061 ggcacgacca tgcgcaacca ggctcaacca caggcacccg acgtcgccac cctcgcgcgc
     6121 cgcctgcgcg accgtctcgc gaacggcgag gcgctgccgg cggaacgcgc actggccgag
     6181 caatacgcgg tcaagcgcca ccgcgtgcgg caggcgctgg cgctgttgcg tgagcagggt
     6241 gatctcgatt cgtcgcgacg caccggcgtc gccgatggcg agtcgctgat ccgctcgacg
     6301 aatccgctcg aggtgatcga ggtgcggctc gcgctggagc cggcgctcgc gcgactcgcg
     6361 gccgtgcgcg cgacgccgct cgaaatcgcg cggatcatgc gcgccgcgac gacaccgtcg
     6421 gacatgcagc gcagcacggc cgacctcgtg ttccacaagc tcgtcgccgc ggccagcggc
     6481 aacggcctcg cggccgatct ctatgcgcta ttgcgcaagg tgggagccga tgcgcgcgtc
     6541 cgggtgcaaa gcagcgcgcc cgcgtgtccc aatcgcctcg cgcagcgcga ccaggagcat
     6601 catggcgtgg ccgaagcgat tgcggcgcgc gatccggaag gcgccgaggc ggcgatgcgc
     6661 gcgcatcttt acgccgtgca tcggatcatc ctgaatcggc tgatggcggc gaacgccgcg
     6721 tgagggtcag ctcgtcagcg ccttcttcga tgcgacgagc agacggcccc gctcgcgcgc
     6781 cagctgctcg atctcctcgt gcgtgtagtc gaacaagcca gcttgcgtct tgagtcccag
     6841 cctgccgtcg ccgaccttgc tcgccaccgt cgtcgaaatg ccggggtcgt tgcaaagatc
     6901 cttgttgagg taaccggcga cggcggtgta gatgtcgaga cccgccatgt cgagcagccg
     6961 cagcgggccg atcacgccga gcttgtagcc gatgccccac ttcacgatgc ggtcgatagc
     7021 ttcggcggac gcaacaccct cgtcgagcag atgcagtgcc tcgcgcatga tcgcgtagag
     7081 gatgcggttc tcgacgaatc ccggaacgtc gcgctcgacg atgcccggca gcatgtgcag
     7141 actctcgacg agggcgaccg ttgaatccac cgtcgacgcc gcggtttcac tgcccttgac
     7201 cacttcgatc accggaatca gatgcggcgg gttcgaccag tgcatgccga tgatgcgccc
     7261 cggattccgc atccccgcct gcagcgtcga gatcggaatg cccgacgtgt cggaggcgag
     7321 gatggcgtcg tcgcgcgccg tggcgcaaag ctccgcgaag atcgcacgct ttagctccag
     7381 ccgctccggc acgttctcga gcacgaagtc ggccgccgcg attgcaccgg ccggctcctg
     7441 caccagcgtc aatcggcttc gcgccgcatc gacttcagcg tcggttgcga atccgccgcc
     7501 ttgcagcgtc gcaaaaacaa ggtcgattcc aacgcgcgcc ttgtcgatct gtttcgcatc
     7561 gctatcgcaa agcgtcgtcg cgtagccgtg acgcgcaaag gtcgccgcga tgccgggacc
     7621 catcgttccg gcgccgacga tgacgacgtt gctgatcttg tcgaccgtca atggacgttt
     7681 ccgtcaaagg gctggatgta ccgtcaatcg cgacgccgat gtcccacgtc gaaatcgttg
     7741 cgcccgctgc ggtcgacacc ccttaccagc cacagcgggc gatgcgcgaa gtcgacggga
     7801 acgccggtcg cgccctcggc gggacgaacg ttgcgctcga acaccgacgt cgcgggcatg
     7861 tagcgcaacg cgacgccggt gcggcgcttg ctcgatcggt tgggcgcggc gccgtgaatc
     7921 atgtacacgt cgtgcagcga catctggccg ggctcgagct cgatgtcgac cgcgtcgcgc
     7981 aactcgaagc tgccggccgc gagccgctgg ttgagcgtga ggtcggtgcg ctcctcgtgc
     8041 agatgctcgt gcaatgtgcg gccgaggtgc gagcgcggga tcacgcgcag acacccgttc
     8101 tcgatggtgg acggctcgag cgcaacccac acggtgcaat tggcgagcgg tcggatcggc
     8161 cagtagtggc catcctgatg ccagggcgtt tcgtagccgt cgccggccgg cttgcagaag
     8221 acgtgacagc cccacaggac gatgtcgtcg ccgatcacgt ccgagacgag ctcgacgatg
     8281 cgcgggtcgc gcgcgagcgc cagaaagcgc gcgtcgccgc gcacgccttc gccgttgtcg
     8341 ccgtcgacgt gcgccgacac gagcttttcg ggtcgcacgc ccgggttggc gcggatcagc
     8401 gcatcgagtg cttcacgcag tcgggcaact tcgtgcggcg gcagccgaaa ccgcggcacg
     8461 acccagcctt cctggcggta atgcgcgatc tcggttgcgt caagccgggc gatggaaggc
     8521 tcgaccgtcg tcatcatggt gtacccgcct gtgtcacgcg agcgattcta gcgcgacgaa
     8581 atgaacgcga cgtctacact ggcgcaaggc cgcggccgca atagcaatga tttccgcatc
     8641 cggacactac ggcgtgcccg gccgatcgcc gtattcgtcg tggcggcgcc accagatgcc
     8701 cgtctcgttg cgccccctcg gtgcgcggtc gagccacggg tacatccccc agagagcgtc
     8761 cagtccgcgc gcatagctcg aataggcgtg gtagacgacg ccgtcgtcga gcgcgaatgc
     8821 gctcatgccg ggacgctcgc gcgtgtacgt ggccgcgtcg gttccgctca tggccgccat
     8881 cttcgcaacc ggcgtgtcgg gcatgcggct cgcgatgccc tcgtcgcttc gcacctgcca
     8941 cgccggctcg tgccggtagt tgtattcgat gccgccttcg cgctgctgct cctcggtgta
     9001 cgtggcgttg aagtcgtagt tgaagtcgct gtcgaacgac gacgcccagg gaaatgtcca
     9061 gcccatccgc cgctcgtacg cctgcaacct ggcgaacgga gcccgcgaca ccgcgcacag
     9121 cgtgacgtcg tgattcgcga ggtggacgac gaaaccgtcg aagccgtccg cgatcgccga
     9181 gcaggacgga cagcctgccg tgtagtcggg cccgaacatg aagtggtaga cgagaagctg
     9241 cgagcgtccc cggaaaaggt ctgcgagcga cgcgcttcct tcgctggtct cgaagcggta
     9301 gtccttgtcg attcggaccc acggcagcgc ctgccgcatc cgcgccagct cgtcgctgcg
     9361 ccgcgtcagc gtcttctcgg cctcgagcag ctggcgccgt gccgcaagcc attcttcgcg
     9421 tgttcccgtc ttgtggtcgg tcaccgtctg tctcctttcg gacgtcgcac ggcgcggacc
     9481 ttgccgctcg ccccgccgcc gcagtagaac aggtctgtcc cgtcggactc gagcccgctg
     9541 acgccggtgc cgtgcggcat ctcgagccgc tcgagcaccg cgccgctggc ggggtcgatg
     9601 cgacggatct cgctctcgtc gccgtcccag gtgccgtgcc acagctcgcc gtcgacccag
     9661 gtcaccccgg tgacgaaacg gttggactcg atggtgcgca aaatggcgcc ggtctgcgga
     9721 tcgacctggt
//
LOCUS       DASSVX010000270         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_595292, whole genome shotgun sequence.
ACCESSION   DASSVX010000270 DASSVX010000000
VERSION     DASSVX010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595292"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFO53_11555"
     CDS             complement(<1..587)
                     /locus_tag="VFO53_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8876956.1"
                     /translation="MGIARRFGDVQALDGVDLRVAQGEFFGLLGPNGAGKTTLISILA
                     GLTRADRGTARILGHDVVTGYRRARRVLGVVPQELVFDPFFTVRETLRIQSGYFGLRH
                     NDAWIDELLHHLDLTHKADANMRALSGGMKRRVLVGQALVHKPPVIVLDEPTAGVDVE
                     LRQSLWAFIRKLNRDGHTIILTTHYLEEAEALCGR"
     gene            complement(692..1006)
                     /locus_tag="VFO53_11560"
     CDS             complement(692..1006)
                     /locus_tag="VFO53_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET8876957.1"
                     /translation="MIEHPACGEPSDGSVTGGDDGWRFAGALTFDNAAQVFAASRALA
                     LPANRSVDFSAMTRADSAALAVMIALKRRAAAQGSALSIEGVPASLRSLAVVYGVENL
                     ID"
     gene            complement(1003..1638)
                     /locus_tag="VFO53_11565"
     CDS             complement(1003..1638)
                     /locus_tag="VFO53_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8876958.1"
                     /translation="MTMPRPLRTGVALCRAGVALLALVAVPALAQEAPDALVKRVSQE
                     ALSIIKSDPRVQAGDQAHIRDVIEAKIAPHFDMDRMTALAAGRAYRQATPEQKKRLAD
                     EFKSLLIRTYSNALNQYRDQTLEYQPLRADPSAAEVTVRTQVIRPGQTPVSIDYSMEK
                     TPSGWKAYDVIVGGVSLVTNYRDEFTQQVRDGGIDGLIKALAAKNAGAPAK"
     gene            complement(1635..2333)
                     /locus_tag="VFO53_11570"
     CDS             complement(1635..2333)
                     /locus_tag="VFO53_11570"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HET8876959.1"
                     /translation="MLLRAAAICAGASLVSACAMGPTREDPFEPVNRVSYKVHEVVDG
                     RIIKPVAQAYADYTPKPVRLAVSNFFGNIDDMFSVINDMLQGKGDKAGNDLGRVITNT
                     GFGVLGLIDVAGSAGIPKGNEDFGQTFGFWGIPQGPYLFIPVFGPTTVRDGSGTLIRA
                     YASPVNYIDDTPTRTALWALNLVDLRASALQTENLLNQAALDPYTFIRRSYLQRRQYL
                     VYDGKPPPPKEDEE"
     gene            complement(2348..2866)
                     /gene="mlaD"
                     /locus_tag="VFO53_11575"
     CDS             complement(2348..2866)
                     /gene="mlaD"
                     /locus_tag="VFO53_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HET8876960.1"
                     /translation="MSRSTIDLWVGIFVTIGIAAILFLALKVGNLTSVSSAPGYRLQA
                     AFDNIGGLKLRAPVKAAGVVVGRVEQIRLDPQTYQAVVSMKIDRGFQFTKDTIASILT
                     SGLLGEVYIGLDSGGDTQMLADDGRIAKTQSAIVLEKLIGQFMFDKAASGTPAGAAAS
                     VDRGAAAAAGAK"
     gene            complement(2916..>3085)
                     /locus_tag="VFO53_11580"
     CDS             complement(2916..>3085)
                     /locus_tag="VFO53_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8876961.1"
                     /translation="FGIAVSAIAVFEGYNARPTAEGVSTATTRTVVASSLSILALDFV
                     LTVFMFRGVHG"
BASE COUNT          486 a         1046 c         1035 g          518 t
ORIGIN      
        1 atgcgcccgc acagcgcctc ggcctcctcg aggtaatgcg tcgtcaggat gatcgtgtgc
       61 ccgtcgcggt tgagcttgcg gatgaacgcc cacaggctct gccgcaactc gacgtcgacg
      121 cccgccgtcg gctcgtcgag cacgatgacg gggggtttgt gcaccagcgc ctggccgacg
      181 agcacgcgcc gcttcatgcc gcccgacagc gcgcgcatgt tggcgtccgc cttgtgtgtg
      241 aggtcgagat gatgcagcag ctcgtcgatc cacgcgtcgt tgtggcgcag gccgaaatag
      301 cccgactgga tgcgcagcgt ttcgcgcacc gtgaagaacg gatcgaacac gagctcctgc
      361 gggacgacgc cgagcacgcg gcgagcacgc cggtaaccgg ttacgacgtc gtggccgagg
      421 atgcgcgccg tgccgcggtc ggcgcgcgtg agtcctgcga ggatcgaaat gagcgtcgtc
      481 ttcccggcgc cgttcggccc gagcaacccg aagaattcgc cctgcgcgac gcggaggtcg
      541 acgccgtcga gcgcctgcac gtcaccgaag cggcgcgcga tgccgatgac ttcgacggcg
      601 tcgcttgtat ggccccccca cgcttgcggc ttcgccgctg cgctgccccc ggagggggcg
      661 ctattcgcgc cttgaggcgg ctctgcggcg ctcaatcgat caggttctcg acgccgtaga
      721 cgaccgcgag cgagcgcagc gaggccggca cgccctcgat cgacaacgcg ctgccctgcg
      781 ccgccgcgcg gcgcttgagc gcgatcatca ccgcgagcgc ggcggaatcg gcgcgggtca
      841 tcgccgaaaa gtcgacgctg cggttcgccg gcagcgcgag cgcacgcgac gcggcgaaaa
      901 cctgcgcggc attgtcgaac gtgagcgcac cggcgaagcg ccagccgtcg tcgccaccgg
      961 tgaccgaacc gtcggatggc tcgccgcacg cgggatgctc gatcatttcg ccggggcgcc
     1021 cgcgttcttc gcggccaacg ccttgatcag cccgtcgatg ccgccgtcgc gcacctgttg
     1081 cgtgaattca tcgcggtaat tggtgacgag cgacacgccg cccacgatca cgtcgtaggc
     1141 cttccagccc gacggcgtct tttccatgct gtagtcgatc gacaccggcg tctgcccggg
     1201 ccggatcacc tgcgtcctga ccgtcacctc ggcggcgctc gggtcggcgc gcagcggctg
     1261 gtactcgagc gtctggtcgc gatattgatt gagcgcgttc gaatacgtgc ggatcaacag
     1321 cgacttgaac tcgtcggcga ggcgcttttt ctgctcgggc gtcgcctggc gatacgcgcg
     1381 gcccgcggcg agcgccgtca tccggtccat gtcgaagtgc ggcgcgatct tcgcttcgat
     1441 cacatcgcgg atgtgcgcct ggtcgcccgc ctgcacccgg gggtcgctct tgatgatgga
     1501 cagcgcttcc tgcgaaacgc gcttgacgag cgcatcgggc gcttcctgcg cgagcgccgg
     1561 cacggcaacg agcgcgagca gcgcgacgcc ggcgcggcac agcgccacgc ccgtgcgcaa
     1621 gggtcggggc atcgtcattc ttcgtcttcc ttcggtggcg gcggcttgcc gtcgtacacg
     1681 agatactggc ggcgctgcag atacgagcgc cggatgaacg tgtacggatc gagggccgcc
     1741 tggttgagca ggttttccgt ctgcagcgcg gaggcgcgca ggtcgacgag gttcagcgcc
     1801 cacagggcag tacgcgtcgg cgtgtcgtcg atgtagttga ccgggctcgc gtaagcacga
     1861 atcagcgtgc cgctgccgtc gcgcaccgtc gtcgggccga ataccgggat gaacaggtag
     1921 ggaccctgcg gaatgcccca gaacccgaac gtctggccga aatcctcgtt gcccttcggg
     1981 atgccggcgc tcccggcgac gtcgatgagg cccagcacgc cgaaaccggt attcgtgatc
     2041 acccgtccga gatcgttgcc ggccttgtcg cccttgccct gcaacatgtc gttgatcacg
     2101 gagaacatgt cgtcgatgtt gccgaagaaa ttgctgacgg cgagccggac gggcttcggc
     2161 gtgtagtcgg cgtacgcctg cgcgacgggc ttgatgatcc tgccgtcgac cacctcgtgc
     2221 accttgtacg acacgcgatt caccggctcg aacgggtcct cgcgcgtcgg gcccatcgca
     2281 caggcgctca cgaggctcgc gcctgcgcag atcgctgcag cacgcagcag catcggtcgg
     2341 tcctccttca tttggcacct gccgcagcgg cggcaccgcg atcgacggaa gccgcggcgc
     2401 ccgccggtgt gccgctcgcc gccttgtcga acatgaactg gccgatcagc ttctcgagca
     2461 cgatcgccga ctgcgtcttg gcaatgcgtc cgtcgtcggc gagcatctgc gtgtcgccgc
     2521 cggaatcgag gccgatgtac acctcgccga gcagccccga cgtcaggatc gatgcgatcg
     2581 tgtccttcgt gaactggaag ccgcggtcga tcttcatcga aaccaccgcc tggtacgttt
     2641 gcggatcgag cctgatctgc tcgacgcgtc cgaccacgac accggcggcc ttcaccggcg
     2701 cgcgcagctt gagcccgccg atgttgtcga acgctgcctg cagccgataa ccgggcgcgc
     2761 tgctgaccga cgtgaggttg ccgaccttca gtgcgagaaa caggatcgcc gcgatgccga
     2821 tcgtcacgaa gatgccgacc cacaggtcga tcgtcgagcg gctcatcgcg tgccggcacg
     2881 gcccgccggg ccgtggcgtc gcctgcgcaa agagatcatc cgtggactcc cctgaacatg
     2941 aatacggtga gcacgaaatc gagcgcgagg atggacagcg agctcgcgac gacggtacgt
     3001 gtggttgcgg tggaaacgcc ttcggccgtc ggccgcgcgt tgtagccttc gaacaccgcg
     3061 atcgcgctga ccgcgatccc gaata
//
LOCUS       DASSVX010000271         8532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1461734, whole genome shotgun sequence.
ACCESSION   DASSVX010000271 DASSVX010000000
VERSION     DASSVX010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461734"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2770)
                     /gene="acnA"
                     /locus_tag="VFO53_11585"
     CDS             complement(<1..2770)
                     /gene="acnA"
                     /locus_tag="VFO53_11585"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HET8876962.1"
                     /translation="MAHNLFDTLKELRLASGATGRYYSLPALEAAGVGNVSRLPVSVR
                     IVLESVLRNCDGKKVTEQHVRQVANWAPNGERSDEIPFVVARVVLQDFTGVPLLCDLA
                     AMRNVAADLGHDPKLLEPLVPVDLVVDHSVMIDHYGSPAALDLNMKLEFNRNAERYQF
                     MKWGMQAFDTFKVVPPGIGIVHQVNLEYLAPGVHAKDGIYYPDTLVGTDSHTTMINGI
                     GVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVELAGRLPEGTTATDLVLTVTEMLRREK
                     VVGKFVEFFGEGTASLSVPDRATIGNMAPEYGATMGFFPVDDATVAYLRATGRSDAAL
                     DAFESYYKAQHLFGMPRAGDIDYTKTLRLDLTTITASVAGPKRPQDRIELPDLAAKFA
                     DLFSKPVGESGFSQPAERLRQRHRTLPMVAGRGQISDAEQQVPRAGMPRDYIEMVNNR
                     PTPDRIAPQAPKAVDIGNGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEKGLTVKPH
                     IKTSLAPGSRVVTDYLTKTGLLPYLEKLGFDVAAYGCTTCIGNAGDLTPEINDAIASH
                     DLVCAAVLSGNRNFEARIHPNLKANFLMSPPLVVAYAIAGTVLRDLAHEPLGEGRAGP
                     VYLRDVWPASQEVDALLNYARDPATFRRLYGDLANTNPMWSTIAGSSGQVYDWPASTY
                     IAKPPFFDGFSMTPPPIGDIRRARVLGIFGDSVTTDHISPAGSIKPTSPAGIYLQEKD
                     VSVASFNSYGARRGNHEVMMRGTFANVRIKNLMLPQRADGTRIEGGVTLHYGRNGATE
                     QMPIYDAAMRYLQDGIPTVVFGGEEYGTGSSRDWAAKGTQLLGVKAVIAKSFERIHRS
                     NLVGMGVLPLQFQPGQGADTLGLRGDETFDIAVPADIKPQQTLTVRMTRADGSRQDIP
                     VLCRIDTPI"
     gene            complement(2784..3344)
                     /locus_tag="VFO53_11590"
     CDS             complement(2784..3344)
                     /locus_tag="VFO53_11590"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HET8876963.1"
                     /translation="MRTFLGVALFATLGLPAFAQDFPRLKPGLWEMERDMGRSSATQP
                     NRPAPSNRTTMCLDESVQNDMFAMAAGAMAGACSRHDFKRSGNRLTGDVVCTLGGSTM
                     HSKSVMTLDGNSAYRTEIDTTYDPPFMGQTHSTMVLRARNVGACKPGQRAGDVVMPNG
                     RTMNMRDMMNGPNGPHGSVSPKAARP"
     gene            complement(3344..4786)
                     /locus_tag="VFO53_11595"
     CDS             complement(3344..4786)
                     /locus_tag="VFO53_11595"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HET8876964.1"
                     /translation="MSSATHSTVRPQPDRVLVDIADYVADYAIESAAAFETAHYCLLD
                     TLGCGFEALSYPACTKLLGPIVPGTFVPHGAKVPGTGYQLDPVHAAFNLGAMIRWLDF
                     NDTWLAAEWGHPSDNLGGILMTADWLSRNAVAQGRAPLTMRDVLTAMIKAHEIQGVIA
                     LQNSFNRVGLDHVVLVKVATTGVVASMLGLSRDEVINALSLAFVDGQSLRTYRHAPNA
                     GSRKSWAAGDATSRGVRLALMAKTGEMGYPSALTATTWGFYDVLFKGQPFRFDRPYGS
                     YVMENVLFKISFPAEFHAQTAVECAMQLHPLVRDRLDAIRRITIRTHESAIRIIDKQG
                     PLANPADRDHCIQYMVAVPLIFGHLVAADYEDDVAADPRIDALREKIVCVEDPQCTQD
                     YLDAGKRSIGNAITIEFDDGSRTPEVAVEYPIGHRRRRAEGIPLLIDKFRINLARRFA
                     AKQQQAILDVALDTARLPAMAVNDFVDLMT"
     gene            complement(4796..5956)
                     /gene="prpC"
                     /locus_tag="VFO53_11600"
     CDS             complement(4796..5956)
                     /gene="prpC"
                     /locus_tag="VFO53_11600"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HET8876965.1"
                     /translation="MTTTEAPVTTIKPKKSVALSGVAAGNTALCTVGRTGNDLHYRGY
                     DILDIAERSEFEEIAYLLVHGKLPTRVELDAYHRKLASLRGIPAPLERMLEAIPASAH
                     PMDVMRTGVSMLGSLLPEHEKHDAAGARDIADRLLASLGSMLLYWYRYSHDGIRIDVE
                     TGDASIGGHFLRLLHRRPPPESWVRAMHTSLNLYAEHEYNASTFAARVIAGTGSDIYS
                     AITGAIGALRGPKHGGANEFSFEVQKRYATPDEAEADIRRRVAAREVVIGFGHPVYTV
                     SDPRNEVIKEVARELSAEAGDTKMFDIAERLETVMWDAKKMFPNLDWFSAVSYHMMGV
                     PTPMFTPLFVMARTAGWAAHVIEQREDGKIIRPSANYVGPEDRPFVPIDQRS"
     gene            complement(5967..6863)
                     /gene="prpB"
                     /locus_tag="VFO53_11605"
     CDS             complement(5967..6863)
                     /gene="prpB"
                     /locus_tag="VFO53_11605"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HET8876966.1"
                     /translation="MVAGGTTPGARFRAAVAAEHPLQVPGAICAYHAILAQRCGYRAI
                     YLSGGGVAAGSLGLPDLGISTLDDVLTDVRRITDACALPLLVDADTGFGASAFNIARS
                     VKSLIKFGAAAMHIEDQVGAKRCGHRPGKELVAQQEMVDRIKAAVDARTDADFVIMAR
                     TDALAVEGLDAAIARARACVDAGADMIFPEAITDLAMYRRFADAVQVPILANLTEFGK
                     TPLFTVEEVAQAGVAIALYPLSAFRAMNAAALNVYRTLRREGTQESVVDTMQTRDELY
                     DCLGYHDYERKLDELFARHKDR"
     gene            complement(6863..7852)
                     /locus_tag="VFO53_11610"
     CDS             complement(6863..7852)
                     /locus_tag="VFO53_11610"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET8876967.1"
                     /translation="MKAPVRVAVTGAAGQIGYSLLPRIASGDMLGDDQPVILQLLELP
                     LDKAQAALKGVMMEMEDCAFPLLAGMTGTSDPRAAFRDADIAMLVGARPRGPGMERKD
                     LLLENAKIFIEQGKALDAVASRDVRVLVVGNPANTNAYIAMKSAPSLPSRHFTAMLRL
                     DHNRALTQLAQKSGKPVGAIEKLIVWGNHSPTMYPDYRFATVDGRSLKDLIDDDAWNR
                     DVFIPTVGKRGAAVIAARGLSSAASAANAAVDHVRDWMLGSGGKWVTMGVPSDGSYAI
                     PPDFVYGVPVVTNDGEYTRVEGLPIDDFSRDRMNATLAELKEERAGVASLLGS"
     gene            8026..>8532
                     /locus_tag="VFO53_11615"
     CDS             8026..>8532
                     /locus_tag="VFO53_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8876968.1"
                     /translation="MRPSPTFRPLYGQVRSMIERALEAGEWAPGEAIPSELDLASRFG
                     VSQGTVRKAVQSLAAENLLVRRQGKGTFVATHTEEKDSNFRFLRIRRNDGKPEYPRSR
                     LVDLRRARASAEAARLLALRTGDPIFVLRRILDYGGVPAVLDEIALPAVLFPGLTHAR
                     CAAYPGSMY"
BASE COUNT         1322 a         2804 c         2944 g         1462 t
ORIGIN      
        1 cgatcggcgt gtcgatgcgg cagagcaccg gaatgtcctg ccggctgccg tcggcgcgcg
       61 tcatcctgac ggtcagcgtc tgctgcggct tgatgtcggc cggcaccgcg atatcgaacg
      121 tttcgtcgcc gcgcagcccc agcgtgtcgg cgccttgccc gggctggaac tgcagcggca
      181 gcacgcccat gccgacgagg ttcgagcgat ggatgcgctc gaagctcttg gcgatcaccg
      241 ccttgacgcc gagcagctgc gtgcccttcg ctgcccagtc gcgcgagctg ccggtgccgt
      301 attcctcgcc accgaagacg accgtgggga tgccgtcctg caggtagcgc atcgccgcgt
      361 cgtagatcgg catctgctcg gtggcgccgt ttctgccgta atgcagcgtg acgccgccct
      421 cgatgcgcgt gccgtcggcg cgctgcggca gcatcaggtt cttgatgcgc acgttcgcga
      481 acgtgccgcg catcatcacc tcgtggttgc cgcgtcgcgc accatagctg ttgaagctcg
      541 cgaccgacac gtccttttcc tgcaggtaga tgcctgccgg cgacgtcggc ttgatcgaac
      601 cggccggcga gatgtgatcg gtggtgaccg aatcgccgaa gatgcccagc acccgcgcgc
      661 ggcgaatgtc gccgatcggc ggcggcgtca tcgagaagcc gtcgaaaaac ggcggcttcg
      721 cgatgtacgt cgacgccggc cagtcgtaca cctgtccgct cgacccggca atggtcgacc
      781 acatcggatt ggtgttcgcg agatcgccgt acagccggcg gaacgtggcc ggatcgcgcg
      841 catagttcaa gagcgcgtcg acttcctgcg acgcgggcca gacgtcgcgc aggtacacgg
      901 gtccggcgcg gccctcgccg agcggctcgt gcgcgaggtc gcgcagcacg gtgcccgcga
      961 tcgcgtacgc gacgacgagc ggcggcgaca tgaggaagtt cgccttgagg ttcggatgga
     1021 tgcgcgcttc gaagttgcgg ttgccggaga gcaccgccgc gcagacgagg tcatgactgg
     1081 cgatcgcatc gttgatctcg ggtgtcaggt cgcccgcatt gccgatgcac gtcgtgcagc
     1141 cgtaggcggc gacatcgaag ccgagcttct cgagataggg aagaagcccg gttttcgtca
     1201 ggtaatcggt gacgacgcgc gacccgggtg ccagcgaggt cttgatgtgc ggcttgacgg
     1261 tgagtccctt ctcgaccgcc ttcttcgcga gcaatcctgc ggcgagcagc acgctcgggt
     1321 tcgacgtgtt cgtgcacgac gtgatcgcgg cgatcagcac atcgccgttg ccgatgtcga
     1381 cggccttcgg cgcttgcggc gcgatgcggt cgggtgtcgg acggttgttg accatctcga
     1441 tgtagtcgcg cggcatgccg gctcgcggca cctgctgctc cgcgtcgctg atctggccgc
     1501 gaccggcgac catcggcagc gtcctgtggc gctggcgcag ccgctcggcc ggctgcgaga
     1561 agccgctttc gccaacgggc ttgctgaaga gatcggcgaa cttcgccgcg agatcgggca
     1621 gctcgatgcg atcctgcggc cgcttgggac ctgcgaccga cgccgtgatc gtggtgaggt
     1681 cgagccgcag cgtctttgtg tagtcgatgt cgccggcgcg cggcatgccg aacaggtgct
     1741 gcgccttgta gtaggactcg aacgcgtcga gcgcggcgtc gctgcggccc gtggcgcgca
     1801 ggtacgcgac ggtggcgtcg tcgacgggaa agaagcccat cgtcgcgccg tactcgggcg
     1861 ccatgttgcc gatggtggcg cgatcgggca ccgacagcga cgcggtgcct tcgccgaaga
     1921 actcgacgaa cttgccgacg accttttccc ggcgcagcat ttcggtgacc gtcagcacga
     1981 gatcggtcgc cgtcgtgccc tcgggcaggc ggcccgcgag ctcgacgccg acgacgtcgg
     2041 gcgtcaggaa gtacaccggc tggcccagca tgcccgcttc ggcctcgatg ccgccgacac
     2101 cccagccgac gacgccgatg ccgttgatca tcgtcgtgtg gctatcggtg ccgaccagcg
     2161 tgtcgggata gtagatgccg tccttcgcgt gcacgccggg ggcgaggtac tcgaggttga
     2221 cctggtgcac gatgccgatg ccgggcggca cgaccttgaa cgtgtcgaac gcctgcattc
     2281 cccatttcat gaactggtag cgctcggcgt tgcggttgaa ctcgagcttc atgttgagat
     2341 cgagcgccgc aggcgagccg tagtggtcga tcatcaccga gtggtcgacg acgaggtcga
     2401 cgggcaccag cggctcgagc agcttggggt cgtgtccgag gtcggccgcg acgttgcgca
     2461 tcgccgcgag gtcgcacagg agcgggacgc cggtgaaatc ctgcaggacg acgcgcgcga
     2521 cgacgaatgg aatttcgtcg ctgcgctcgc cgttcggcgc ccagttcgcc acctgccgca
     2581 cgtgctgctc ggtgaccttc ttcccatcgc aattgcgcag cacgctctcg agcacgatgc
     2641 gcaccgacac gggcagccgc gacacgttgc cgacgccggc cgcctcgagc gcgggcaatg
     2701 agtaatagcg gcccgtcgca ccggatgcga gccgcagttc cttcagcgtg tcgaacaggt
     2761 tgtgtgccat cgtggtgtcc cggctagggg cgcgcagcct tcggcgatac gctcccatgg
     2821 ggtccgttcg gaccgttcat catgtcccgc atgttcatcg tgcgaccgtt cggcatgacg
     2881 acgtcgccgg cgcgctgccc gggcttgcac gccccgacgt tgcgcgcgcg cagcaccatc
     2941 gtcgagtgcg tctgccccat gaacggcggg tcgtacgtcg tgtcgatctc ggtgcgatag
     3001 gcgctgttgc cgtcgagcgt catcaccgac ttcgaatgca tcgtgctgcc gccgagggtg
     3061 cagacgacgt cgccggtcag gcgattgccg gaccgcttga aatcgtggcg gctgcacgca
     3121 cccgccatcg caccggcagc catggcgaac atgtcgtttt gcaccgattc gtcgaggcac
     3181 atggtcgtgc gattcgaagg tgccgggcga ttcggctgcg ttgcggacga gcggcccatg
     3241 tcgcgctcca tttcccacag gccgggcttc agccgcggaa agtcctgcgc gaacgcggga
     3301 aggccgaggg tcgcgaaaag cgccacaccg aggaaggtgc gcatcacgtc atcaaatcca
     3361 cgaaatcgtt caccgccatc gcaggcaggc gcgccgtatc gagcgcgaca tcgaggatcg
     3421 cctgctgctg cttcgcggcg aaccggcgcg cgaggttgat ccggaacttg tcgatcaaca
     3481 gcggtatgcc ttcggcgcgc ctgcgccggt gcccgatcgg atactcgacg gcaacttcgg
     3541 gggtgcggct gccgtcgtcg aactcgatcg tgatcgcatt gccgatcgag cgcttgccgg
     3601 cgtcgagata atcctgcgtg cattgcgggt cctcgacgca gacgatcttt tcgcgcaacg
     3661 cgtcgatgcg cggatcggcg gcgacgtcgt cctcgtaatc ggcggcgacg agatgcccga
     3721 agatcagcgg caccgcgacc atgtactgga tgcagtggtc gcgatcggcc ggattggcga
     3781 gcgggccctg cttgtcgatg atgcggatcg ccgattcatg cgtgcgtatc gtgatgcgcc
     3841 ggatcgcgtc gagccggtcg cggaccagcg gatgcagctg cattgcacat tcgaccgcgg
     3901 tctgcgcgtg gaattcggcc gggaagctga tcttgaagag cacgttctcc atcacgtagc
     3961 tgccgtaggg ccggtcgaag cggaatggct ggcccttgaa cagcacgtcg taaaagcccc
     4021 aggtcgtcgc cgtcagcgcc gacggatagc ccatttcgcc ggtttttgcc atcagcgcaa
     4081 ggcgcacgcc gcgcgacgtc gcatcgccgg ccgcccagct cttgcgcgag ccggcgttcg
     4141 gcgcgtgacg atacgtgcgc aggctctgtc cgtcgacgaa ggcgagcgac agcgcgttga
     4201 tcacctcgtc gcgcgacagc ccgagcatgc tggccacgac gccggtcgtg gcgaccttca
     4261 ccagcacgac gtggtcgagg ccgacgcgat tgaagctgtt ctgcaatgcg atgacgccct
     4321 ggatctcgtg cgccttgatc attgccgtca gcacgtcgcg catcgtcagc ggcgcgcggc
     4381 cctgcgcaac cgcgttgcgc gacagccagt cggcggtcat caggatgccg cccaagttgt
     4441 cggaggggtg gccccattcg gcggcgagcc acgtgtcgtt gaaatcgagc cagcggatca
     4501 tcgcgccgag gttgaacgcc gcgtgcaccg ggtcgagctg gtagccggtt cccggcactt
     4561 tggcgccgtg cgggacgaag gtccccggca cgatcggccc gagcagcttg gtgcaggcgg
     4621 gataggacag cgcctcgaag ccgcagccga gcgtgtcgag caggcagtag tgcgcggttt
     4681 cgaaggccgc ggcgctttcg atagcgtaat cggcaacgta gtcggcgata tcgaccagca
     4741 cccggtcggg ttgcgggcgg acggtgctgt gggtggcgga ggacatggct tcgattcagg
     4801 atcgctgatc gatcggcacg aacggccgat cttcgggtcc gacgtaattc gccgacgggc
     4861 ggatgatctt gccgtcctcg cgctgctcga tcacgtgcgc agcccacccg gccgtgcgcg
     4921 ccatgacgaa cagcggcgtg aacatcggcg tgggcacgcc catcatgtgg tagctcaccg
     4981 ccgagaacca gtcgaggttc ggaaacatct ttttcgcatc ccacatcacg gtttcgagcc
     5041 gctcggcgat gtcgaacatc ttcgtgtcgc cggcttcggc cgacagctcg cgcgcaacct
     5101 ccttgatcac ctcgttgcgc ggatcggaca ccgtgtacac cggatggccg aagccgatga
     5161 ccacctcgcg cgcggcgaca cgccggcgga tgtcggcttc ggcttcgtcg ggcgtcgcgt
     5221 aacgcttctg cacctcgaac gagaactcgt tcgcgccgcc gtgcttcggt ccgcgcagcg
     5281 cgccgatcgc gccggtgatc gccgaataga tgtcggagcc ggtgccggcg atgacgcgcg
     5341 cggcaaacgt cgacgcgttg tactcgtgtt cggcatacag gttgagcgac gtgtgcatcg
     5401 cgcgcaccca cgactcgggc gggggacgcc ggtgcagcag acgcaggaaa tggccgccga
     5461 tcgacgcgtc gccggtttcg acgtcgatgc gaatgccgtc gtggctgtag cgataccagt
     5521 agagcagcat cgagccgagc gacgccagca gccggtcggc gatgtcgcgc gcgccggcgg
     5581 catcgtgctt ctcgtgctcg ggcagcaggc tgccgagcat cgatacgccc gtgcgcatca
     5641 cgtccatcgg atgcgccgac gcgggaatgg cctcgagcat gcgttcgagc ggggcgggga
     5701 tcccgcgcag cgacgcgagc ttgcggtgat acgcgtcgag ctcgacgcgg gtcggcagct
     5761 tgccgtgcac gagcagatag gcgatttcct cgaattccga tcgctccgcg atgtcgagga
     5821 tgtcgtagcc gcggtagtgc agatcgttgc cggtgcgccc gacggtgcat agcgcggtat
     5881 tgccggcggc gacgccggac agcgcaaccg acttcttggg cttgatcgtc gtgaccggcg
     5941 cttctgtcgt cgtcatcggt ggaggctcag cggtccttgt ggcgtgcgaa aagctcgtcg
     6001 agcttccgct cgtagtcgtg atagccgagg cagtcgtaca gctcgtcgcg cgtctgcatc
     6061 gtgtcgacca cgctttcctg cgtgccctcg cggcgcagcg tccggtagac gttcaacgcc
     6121 gcggcgttca tcgcccggaa tgccgacagc ggatagagcg cgatggccac acccgcttgc
     6181 gcgacctctt cgaccgtgaa aagcggcgtc ttgccgaatt cggtgaggtt ggcgaggatc
     6241 ggcacctgca cggcgtcggc gaaccggcga tacatggcga gatcggtgat cgcttcgggg
     6301 aagatcatgt cggcgccggc gtcgacacac gcgcgggcgc gggcgatcgc cgcatcgagc
     6361 ccctcgacgg cgagcgcatc ggtgcgcgcc atgatgacga aatcggcatc ggtgcgcgcg
     6421 tcgaccgccg ccttgatccg gtcgaccatc tcctgctgcg cgacgagctc cttgcccggc
     6481 cggtggccgc agcgcttggc gccgacctgg tcctcgatgt gcatggcggc cgcaccgaac
     6541 ttgatcagcg acttcaccga gcgcgcaatg ttgaatgccg acgcaccgaa gccggtgtcg
     6601 gcgtcgacga ggagcggcag cgcacaggca tcggtgatgc gccgcacgtc ggtcagcacg
     6661 tcgtcgagcg tgctgatgcc aaggtcgggc agcccgagcg agccggcagc gacgccgccg
     6721 ccggacaggt agatcgcgcg atagccgcag cgctgcgcga ggatcgcgtg atacgcgcag
     6781 atcgcgcccg gcacctgcag cggatgctcg gccgcgaccg cagcgcggaa ccgcgcaccc
     6841 ggcgtcgtgc cgcccgcaac gatcacgagc cgagcagcga cgcgacgccc gcgcgctctt
     6901 ccttgagctc cgcgagcgtc gcgttcatgc ggtcgcgcga gaagtcgtcg atcggcagtc
     6961 cctcgacgcg cgtgtattcg ccgtcgttcg tgacgaccgg cacgccgtag acgaaatcgg
     7021 gcgggattgc gtagctgcca tcggagggca cgcccatcgt cacccacttg ccgccgctgc
     7081 cgagcatcca gtcgcgcacg tgatcgaccg cggcgttcgc cgccgacgcc gccgacgaca
     7141 gcccgcgggc ggcgatgacc gcggcgccgc gcttgcccac ggtgggaatg aacacgtcgc
     7201 ggttccacgc gtcgtcgtcg atcaggtcct tgagcgagcg gccgtccacc gtggcgaacc
     7261 ggtaatcggg gtacatcgtc ggcgaatggt tgccccagac gatcagcttc tcgatcgcgc
     7321 cgacgggctt cccgcttttc tgcgcgagtt gcgtcagtgc gcggttatgg tcgagccgca
     7381 gcatcgccgt gaagtgccgg ctcggcagcg acggcgccga cttcatcgcg atgtacgcat
     7441 tggtgttggc cgggttgccg acgaccagca cgcgcacgtc gcgcgaggcg acggcgtcga
     7501 gcgccttgcc ctgctcgatg aagatcttcg cgttctcgag cagcaggtcc ttgcgctcca
     7561 tgccggggcc gcgcggacgc gcgccaacga gcatggcgat gtcggcatcg cggaacgcgg
     7621 cgcgcggatc ggacgtgccg gtcatgccgg cgagaagcgg gaaggcgcag tcctccattt
     7681 ccatcatcac gcccttcagc gccgcctgcg ccttgtcgag cggcagctcg agcagctgca
     7741 ggatcaccgg ctggtcgtcg ccgagcatgt cgcccgacgc aatgcgggga agcaggctgt
     7801 agccgatctg gccggcggcg ccggtcacgg cgacgcgaac gggtgctttc atcacggact
     7861 cctgaaacct ggaactgcgg atgagacgcc gggtcgggcc ggcgccgcag ttcgcgcctc
     7921 gctgcagcgc cgcgaacgcc ggattgccct cggggcacga gttgtcaatg gtaggcgcgc
     7981 gccccgggta ctgtcaatgt cagctaactg atataagata caagagtgcg accttccccg
     8041 acgtttcgtc ccctctacgg ccaggtgcgc tcgatgatcg agcgcgcgct ggaggccggt
     8101 gagtgggcgc ccggcgaggc gattccgtcc gagctcgatc tcgcctcgcg cttcggcgtg
     8161 tcgcagggca ccgtgcgcaa ggccgtccag tcgctcgccg ccgagaacct gctcgtccgc
     8221 cggcagggca agggaacgtt cgttgcaacg cataccgagg aaaaggactc gaatttccgt
     8281 ttcctgcgca tccggcgcaa cgacggcaag cccgagtatc cgcgaagccg gctggtcgat
     8341 cttcggcgcg cgcgcgccag cgccgaggcg gcacggctcc tggcgcttcg aaccggcgat
     8401 cctatcttcg tcctgcgccg gattctcgac tacggcggcg tgccggccgt gctcgacgag
     8461 atcgcgctgc ccgccgtgct ctttccggga ctcacgcacg cccgctgcgc ggcctacccg
     8521 gggtcgatgt ac
//
LOCUS       DASSVX010000272         5891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_345359, whole genome shotgun sequence.
ACCESSION   DASSVX010000272 DASSVX010000000
VERSION     DASSVX010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345359"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..543
                     /locus_tag="VFO53_11620"
     CDS             226..543
                     /locus_tag="VFO53_11620"
                     /inference="COORDINATES: protein motif:HMM:NF023508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3563 family protein"
                     /protein_id="HET8876969.1"
                     /translation="MRTYEISSPGAAELYFNNSFVGDVASLVGAALAGFGGGRGHTRR
                     DRPGATARSAAQKRGLLDRLDAWFWRQQQKAQEDYLAGARDVYDLERRIDALDRGSVT
                     RYY"
     gene            complement(581..1858)
                     /gene="glp"
                     /locus_tag="VFO53_11625"
     CDS             complement(581..1858)
                     /gene="glp"
                     /locus_tag="VFO53_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET8876970.1"
                     /translation="METTRTLRDVSCADDYDPDSLPVDRARTLIREFLSPVSVVERVA
                     IRAALDRVLAADVESPLDVPGHDNSAMDGWAVRFADLDGRSETNLRRIGESFAGKPFA
                     GELAAGETVRIFTGGVMPRGADTVVMQERAQETAHGVAIAPGAVTKAGQNRRFAGEDL
                     KRGGVVFRKGQPIRPAELGMLASLGIGEVSVYRRLRVAFFSTGDELRSVGQPLAAGEI
                     YDSNRYTLYGMLTRLNCDILDMGVVPDAPELLEKAFASAAASADVVITSGGVSVGEAD
                     YVKGLLDRLGEVLFWKIAMKPGRPLAYGRIGAAHFFGLPGNPVSVMVTFYEFVRDALL
                     VLQGRADVAPMPMFKARLAAPIRKMPGRTEFQRAVLLPGDAGGWQVRTTGDQGSGILS
                     SMSRANCFVVLPADTGNLDAGAEVDVQLLEGLI"
     gene            complement(1875..2471)
                     /gene="mobA"
                     /locus_tag="VFO53_11630"
     CDS             complement(1875..2471)
                     /gene="mobA"
                     /locus_tag="VFO53_11630"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HET8876971.1"
                     /translation="MTIERQAITGVVLAGGQGRRMGNVDKGFVELDARPLIAHVIDRL
                     VPQVATLVINANRSAERYAAFGYPVVGDAVGGFAGPLAGLHAGLAAAATPFVATSPCD
                     SPFLPADLVARLAAAFDARPIDIAVARTFDQPHPVFALVRRTVLPHLAQFLDGGGRKI
                     DAWYASLAIAEVSFDDEADAFRNINTPADLAQSAAAAH"
     gene            complement(2468..3304)
                     /locus_tag="VFO53_11635"
     CDS             complement(2468..3304)
                     /locus_tag="VFO53_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HET8876972.1"
                     /translation="MLAPASASPVLTHAARPSTFDVTAVDENGAVRDVPIAGEHPLTL
                     YVDKQEILTLMTLGAAPEALAIGYLRNQRLVQSLDEIVAVQVDWETNSVAVRTRHGLA
                     DLEAKTSKRTKTSGCGQGTVFGDLMEDIDNVSLPQDATLAKSALYALLDKVRTHETIY
                     KQAGAVHGCALASNAGTHSEILQFVEDVGRHNAVDAIAGQMWLDACAGDDKIFYTTGR
                     LTSEMVIKAAQMRIPFLVSRSGLTQMGYEIAQKVGMTMIGRATNRHYLLFTGAHRFRP
                     DR"
     gene            complement(3311..4420)
                     /gene="moaA"
                     /locus_tag="VFO53_11640"
     CDS             complement(3311..4420)
                     /gene="moaA"
                     /locus_tag="VFO53_11640"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET8876973.1"
                     /translation="MATVIPISDVRGASSAAERDAFVRAAANAPAPGDVSSPRDLRGR
                     ALQDLRISVTDRCNFRCVYCMPKDVYGRDFPFLAHAELLTFEEIARIAAIFVERGVRK
                     IRLTGGEPLLRRHLERLVEMLASLGDIDLTLTTNGALLAKKARALRDAGLSRVTVSLD
                     ALDDPTFRAMNDVDFPVARVLEGIDAAHAAGLTPLKINAVVKRGANEHAIVPLARHFR
                     DSGHIVRFIEYMDVGHTNGWRMDHVVPAAEIIAMIDREFALEPIDPNYTGEVAQRWRY
                     RDNGREVGVIASVTQAFCRDCTRARLSTDGKLYTCLFADSGYDLRALLRAPYSDAQIG
                     NAIGAIWRGRADRYSEIRSAATRRDSRIEMSYIGG"
     gene            4528..5049
                     /locus_tag="VFO53_11645"
     CDS             4528..5049
                     /locus_tag="VFO53_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HET8876974.1"
                     /translation="MRPLTQNVDVMIERVPLRSRWASERWQPVAIEIAPPAPGFVPDR
                     DAVPGSHLCIPEGENRWRCRGFEIELHPTEAEGYFLNLTAPDPRIFVMWRLFDDGAMP
                     AARPVLLTVSYNQAGRYMDGGEQVDGVPMLPAIADWMRPFIAAHYKPEPRRKVRRNDP
                     VGDDPVSRRRSEH"
     gene            5049..5765
                     /locus_tag="VFO53_11650"
     CDS             5049..5765
                     /locus_tag="VFO53_11650"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HET8876975.1"
                     /translation="MADTPHPTGFSLRRWSQRKLAAGQRSESARTGDRAPAGPAAKPE
                     GNRAIAPRAGTNPEQAANRGHPMGSAPSHSAGSGAAAPAIAETGNAVALPPLESLTID
                     SDFSPFMQPGVDEGVRRSALRKLLRDPRFNVMDGLDVYIDDYSTPSPLEPALARTLMQ
                     ARYIFDPPKTRVTAEGIVEDVPEGEDAVAADDPGAVEALPPSAPVAIEASADATVPAE
                     SPAAEAGTVPLATSTTVNSR"
BASE COUNT          971 a         2012 c         1991 g          917 t
ORIGIN      
        1 gagctcggcc accgccgcga agcttcgcag ctggtagagc tccacggcta ggggcgggtg
       61 gtcgtcatgc agtgcaatac gacgtcgaat gtccgatcaa gttcacttat gtccctgatc
      121 cggaactatg atttgatcaa agcgcgagcg gcctctacat tgtaattgtg cagtgcaaca
      181 gactgcgcac aaggaacaga gccccatgag ggcatggaga aagctatgcg tacttatgaa
      241 atttcgtcgc cgggtgcggc cgaactgtac ttcaacaaca gcttcgtcgg cgatgtcgcc
      301 tcgctggtcg gcgcggcgct ggcgggcttc ggcggcggtc gcggccatac ccgccgggac
      361 cgtcccggcg ccaccgcgcg ctccgcggcg caaaagcgcg gcctgctcga tcgcctcgat
      421 gcgtggttct ggcgccagca gcagaaggcg caggaagact accttgctgg cgcgcgcgac
      481 gtgtacgacc tcgagcggcg catcgatgcg ctcgatcgtg gcagcgtgac gcgctactac
      541 tgacccggcc gcccggagcc ttggggcggc ccggcggctc tcatatcagc ccctcgagca
      601 gctgcacgtc gacctcggcg cccgcgtcga ggttgccggt gtcggccgga agcacgacga
      661 agcagttcgc gcgcgacatc gacgacagga tgcccgagcc ctgatcgccg gtggtgcgaa
      721 cctgccagcc accggcatcg ccgggcaaaa ggaccgcgcg ctggaactcg gtgcgtcccg
      781 gcatcttcct gatcggcgcg gcgagccgcg ccttgaacat cggcatcggc gcaacatcgg
      841 cgcgtccctg caggacgagc agcgcgtcgc gcacgaattc gtagaacgtc accatcacgg
      901 aaaccgggtt gcccggcaga ccgaagaaat gcgcggcacc aatgcggccg taggcgagcg
      961 ggcggccggg cttcatcgcg atcttccaga acagcacctc gccgagccgg tcgagcaacc
     1021 ccttcacgta atcggcttcg ccgaccgaca cgccacccga cgtgatcacg acgtcggccg
     1081 atgcggcggc gctcgcaaac gctttttcga gcaattcggg cgcgtcgggc acgacgccca
     1141 tgtcgagaat gtcgcagttg agccgcgtca gcatcccgta cagcgtgtac cggttgctgt
     1201 cgtagatctc gccggccgcg agcggctggc cgaccgagcg cagctcgtcc cccgtggaga
     1261 agaacgcgac gcgcaggcgc cggtacacgc tcacttcgcc gatgccgagt gacgccagca
     1321 tgccgagctc ggccggccgg atcggctgtc ccttgcggaa tacgacgccg ccgcgcttca
     1381 ggtcctcgcc ggcgaagcga cggttctgcc ccgccttcgt caccgcgccc ggtgcgatcg
     1441 cgacgccgtg cgccgtttcc tgcgcgcgct cctgcatcac cacggtgtcg gcgccgcgcg
     1501 gcatcacgcc gccggtaaag atgcgcaccg tttcaccggc ggcaagctca cccgcgaacg
     1561 gcttgccggc gaacgactcg ccgatgcggc gcaggttcgt ttcggaccgc ccgtcgaggt
     1621 cggcgaagcg caccgcccat ccgtccatcg ccgaattgtc gtggccggga acgtcgagcg
     1681 gcgattcgac gtcggcggcg agcacgcgat cgagcgccgc acggatcgcc acccgctcga
     1741 cgacgctgac cggcgacagg aactcgcgga tcagcgtgcg cgcacggtcg accggcagcg
     1801 agtcgggatc gtaatcgtcg gcgcacgaga cgtcgcgcag cgtccgggtc gtttccatgg
     1861 gaggacgatt catttcagtg cgccgccgcg gcgctctgcg cgaggtccgc gggtgtgttg
     1921 atgttgcgaa acgcgtcggc ctcgtcgtcg aagctcacct cggcgatcgc cagcgatgca
     1981 taccacgcat cgatcttgcg gccgccgccg tcgaggaatt gcgcgaggtg cggcagcacc
     2041 gtgcggcgca ccagcgcgaa gacgggatgc ggctggtcga acgtgcgcgc gacggcaatg
     2101 tcgatcggcc gcgcgtcgaa cgcggcggcg agacgcgcga cgaggtcggc cggcaggaag
     2161 ggcgagtcgc agggactcgt cgcgacgaac ggcgtcgctg ccgccgcgag gccggcgtgc
     2221 aatccggcga gcggacccgc gaacccgccg accgcgtcgc cgacgacggg ataaccgaac
     2281 gctgcatagc gttccgcact ccggttcgcg ttgatgacga gcgttgcgac ctgcggcacg
     2341 agccggtcga tcacatgcgc gattagcggg cgtgcgtcga gctcgacgaa acccttgtcg
     2401 acgttgccca tgcgccgccc ctgtccaccg gcaagcacca cgcccgtaat ggcctgccgc
     2461 tcgatggtca tcgatccggc cggaagcgat gcgcgccggt gaacagcagg taatggcgat
     2521 tcgtcgcacg gccgatcatc gtcatgccca ccttctgcgc aatctcgtaa cccatctgcg
     2581 tcagccccga gcgcgacacg aggaacggaa tgcgcatctg cgccgccttg atcaccattt
     2641 cagacgtgag ccgcccggtc gtgtagaaga tcttgtcgtc gcctgcgcac gcgtcgagcc
     2701 acatctggcc ggcgatcgcg tcgacggcgt tgtggcggcc gacgtcctcg acgaactgca
     2761 ggatttccga atgcgtgccg gcgttcgacg cgagtgcgca accgtgcacg gcacccgcct
     2821 gcttgtagat cgtctcgtgc gtgcgcacct tgtcgagcaa cgcgtagagc gccgatttcg
     2881 cgagcgtcgc atcctgcggc aggctcacgt tgtcgatgtc ttccatcagg tcgccgaaca
     2941 cggttccctg gccgcagccg ctcgtcttcg tgcgcttcga cgtcttcgcc tcgaggtcgg
     3001 caaggccgtg acgcgtcctg accgccaccg agttcgtctc ccagtcgacc tggaccgcaa
     3061 cgatctcgtc gaggctttgc accagccgct ggttgcgcag gtacccgatc gcgagcgcct
     3121 cgggtgccgc gccgagggtc atcagcgtca ggatttcctg cttgtcgacg tagagggtca
     3181 gcggatgctc gccggcgatc ggcacgtcgc gcacggcacc gttttcgtcg acggcggtga
     3241 cgtcgaacgt cgacggacgc gcggcgtgcg tgaggacggg cgacgccgag gcaggcgcga
     3301 gcatcgcttg ctagcccccg atgtacgaca tctcgatgcg cgagtcgcgc cgggtcgccg
     3361 cgctgcggat ttcggagtac cggtcggcgc ggccgcgcca gatggcgccg atcgcattgc
     3421 cgatctgtgc gtcggaatac ggcgcgcgca acagtgcgcg cagatcgtag ccgctgtcgg
     3481 cgaaaaggca ggtgtagagc ttgccgtcgg tcgacagccg cgcacgcgtg cagtcgcggc
     3541 agaacgcctg cgtcaccgag gcgatgacgc cgacctcgcg gccattgtcg cgatagcgcc
     3601 accgctgcgc gacctcgccc gtgtagttgg ggtcgatcgg ctcgagcgca aattcgcggt
     3661 cgatcatcgc gatgatctcg gcagccggaa cgacgtggtc catccgccag ccgttggtgt
     3721 ggccgacgtc catgtactcg atgaagcgca cgatgtgccc gctgtcgcgg aaatgacgtg
     3781 cgagcggcac gatcgcatgc tcgttggcgc cgcgcttgac caccgcgttg atcttgagcg
     3841 gcgtgaggcc cgcggcatgc gcggcatcga tgccctccag cacgcgcgcg accgggaagt
     3901 cgacgtcgtt catcgcgcga aacgtcggat cgtcgagcgc gtcgaggctg accgtgaccc
     3961 gcgacagccc cgcgtcgcga agcgcgcgcg cctttttggc gagcagtgcg ccgttcgtgg
     4021 tcagcgtcag gtcgatgtcg ccgagcgacg ccagcatctc gaccagccgc tcgaggtggc
     4081 ggcgcagcag cggctcgcca cccgtgagcc ggatcttgcg cacgccgcgc tcgacgaaga
     4141 tcgcggcgat gcgcgcaatc tcctcgaacg tcaaaagctc ggcatgcgcg agaaacggga
     4201 agtcgcgtcc gtagacgtcc ttcggcatgc agtaaacgca gcggaagttg cagcggtcgg
     4261 tgaccgagat gcgcaggtcc tgcagcgccc gcccgcgcag gtcgcggggc gacgacacgt
     4321 cgccaggcgc cggcgcgttc gcggccgcgc ggacgaaggc gtcccgttcg gcggcgctcg
     4381 acgcgccgcg cacatcggag atcggaatga ccgtggccat ggggactgtc gatgttagca
     4441 gaggcgcctg ttcggtttgc tgcggctgta tgatcggcta tgcacgattt cggtgcacga
     4501 agcgcactgc gaagaacccc ggacaggatg cgaccgctga cccagaacgt cgacgtgatg
     4561 atcgagcgtg taccgcttcg aagccgctgg gccagcgaac gctggcagcc ggtggcgatc
     4621 gaaatcgcgc cgcccgcgcc cgggttcgtt ccggatcgcg acgccgttcc cggctcgcac
     4681 ctttgcattc ccgaaggcga gaaccgctgg cgctgccgcg gcttcgagat cgagctgcat
     4741 ccgacggaag ccgagggcta ctttctcaac cttaccgcgc ccgatccgcg catcttcgtc
     4801 atgtggcgac tgttcgacga cggcgcaatg cccgccgcgc ggccggtgct gctgacggtg
     4861 agctacaacc aggcgggccg ctacatggat ggcggcgaac aggtcgacgg cgtgccgatg
     4921 cttcccgcga tcgccgactg gatgcggccg ttcattgccg cgcattacaa gcccgagccc
     4981 cggcgcaagg tgcgccgcaa cgacccggtg ggcgacgatc cggtatcacg acgcaggagc
     5041 gagcactagt ggccgacacg ccgcacccga ccggtttctc gctgcgtcgc tggtcgcagc
     5101 gcaagctcgc tgcggggcag cgcagcgaat cggcgcgaac cggggatcgc gcgcctgccg
     5161 ggcccgcggc gaaaccggag ggcaatcgcg ccatcgcgcc acgcgccggc acgaatccgg
     5221 aacaggcggc gaaccgcggt catccaatgg gctcggcacc gtcacattcg gcgggttcag
     5281 gcgccgcggc gccggcaatc gccgagaccg gcaacgccgt tgcactgccg ccgctcgaat
     5341 cattgacgat cgattccgat ttttcgccgt tcatgcagcc cggcgtcgac gaaggcgtga
     5401 ggcgcagcgc gctgcgcaag ctccttcgcg atccccggtt caacgtgatg gacgggctcg
     5461 acgtgtacat cgacgactat tcgacgccga gcccgctcga gcccgcgctc gcgcgcacgc
     5521 tgatgcaggc gcgctacatc ttcgatccgc cgaagacgcg cgtgacggcc gaaggaatcg
     5581 tcgaggacgt tcccgagggc gaagacgccg ttgccgcgga cgatcccggc gccgtcgagg
     5641 cgctgccgcc ttctgctccc gttgcaatcg aggcgagcgc ggatgcaacc gttcccgccg
     5701 aatctcccgc tgcggaagcc ggcacggtgc cgttggcaac ctccaccacc gtgaacagcc
     5761 gatgaccctc gccgacaaga acgttttcct gtgcaactgc aacggcacga tgccgctcga
     5821 cgccgacgcc cttgcgcgtg cggcaggaaa cgcggcgaag ccggtgcata cggcgatgtg
     5881 ccagcgcgag c
//
LOCUS       DASSVX010000273         7469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_761704, whole genome shotgun sequence.
ACCESSION   DASSVX010000273 DASSVX010000000
VERSION     DASSVX010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761704"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VFO53_11655"
     CDS             complement(<1..943)
                     /locus_tag="VFO53_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8876976.1"
                     /translation="MAPDTLERPALDLADPSLLRQQCHVDGAWLAADDGATMPVVDPA
                     SGALIGTAPVFRAAETKRAIDAAHRAFPTWRAKTAKERSGVLRKWNDLMLAHADDLAR
                     ILTAEQGKPLAEAKGEITIGAAYVEWFAEEAKRIYGDVIPTMASDRRLVVVKEPVGVC
                     AAITPWNFPSSMITRKVSPALAAGCTVVIKPAEATPFSAFALAELAARAGFPPGVLNV
                     ITGDAQSIGAEMCANPIVRKLSFTGSTRVGRLLMQQVAPTIKKISLELGGNAPFIVFD
                     DADLDAAAEGAMISKYRNAGQTCVCANRFFVHEKVYES"
     gene            complement(981..1913)
                     /locus_tag="VFO53_11660"
     CDS             complement(981..1913)
                     /locus_tag="VFO53_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8876977.1"
                     /translation="MGVLFAVLGVLGFSFKAILIKLAYRWAPIDPVALMTLRMIYSAP
                     FFLLMAWWSGRVPGIARMSARDVRLLIGLGFIGYYLSSLLDFIGLQYVTASLERLVLF
                     LYPTIVVVLSALFLGQPITRRAVAALVLSYAGIALAVFHDVRVTGDPRAIAFGSALVF
                     ASAVGYAIYLVGATGIIGRLGPSRFIAWAMLAATAFISLHFALTRPFVALAAPQSIQL
                     LALAMAFFCTVLPTWMIAESIRRIGASTSSLVGSLGPMFTIGFGALLLDEPINVLQMI
                     GVVLVLAGVMLVSRGAGRPRAAPAGPNAVRRGTV"
     gene            complement(1971..2801)
                     /locus_tag="VFO53_11665"
     CDS             complement(1971..2801)
                     /locus_tag="VFO53_11665"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET8876978.1"
                     /translation="MDHPSALVALILGIVEGLTEFLPVSSTGHLIVAGSFLGYTGERA
                     KVFEIVIQAGAILAVCWEFRARLIGVVSGLGSDPVAQRFVVNLIVAFIPAAVLGLLFA
                     KAIKRHLFAPLPVALAFIAGALVILWVERRMRVAPSRVRIDDVDDMRWLDALKIGIAQ
                     SFALIPGTSRSGATIIGGMLFGLSRPAATRFSFFLAIPTLLAACAYDFLRNRALFSVQ
                     DLQGLAIGFVAAFVAAFLCVRWLIRYVSRHDFVPFAWYRIAFGLLILVTAWMGLVDWT
                     "
     gene            2873..3574
                     /locus_tag="VFO53_11670"
     CDS             2873..3574
                     /locus_tag="VFO53_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8876979.1"
                     /translation="MDHELHQLFDTESSTFTYVLVDRASREAILVDSVEQRFERDMSL
                     LRRLALTLRYVVETHTHADHVTAAGRLRDATGAKTVAPFKCGISPADLQLAHGDTLHF
                     GAGETVHAIHTPGHTSGSTSYLWRGNVLTGDTLLIEGCGRTDFQSGDAGSLYDSVHER
                     LFTLPDDTRVWPAHDYRGNTVSTIGHEKRHNARLVGRDRASFIELMGNLQLPKPKLID
                     VAVPANRLLGLTHAV"
     gene            complement(3619..4287)
                     /locus_tag="VFO53_11675"
     CDS             complement(3619..4287)
                     /locus_tag="VFO53_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HET8876980.1"
                     /translation="MTPPRLSVAEARVLGVLVEKQRTVPDTYPLTLNALVAGCNQRSS
                     RDPVLTLTDTEVQQALDDLKARSMAMETSGGRVMRYAHNAERALALPSQSVAILAALL
                     LRGPQTAGELRIGTERMHRFADISTVEAFLNELAERQAGALVAQLPRSPGTRETRWMQ
                     LLSEPALPPAPGAAASSALDAPRDSPTLAQDVDLLRDEVAVLRNELAALRVEIEALRS
                     ERGG"
     gene            4357..5202
                     /locus_tag="VFO53_11680"
     CDS             4357..5202
                     /locus_tag="VFO53_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET8876981.1"
                     /translation="MANTPEVTSMALFCDFENIALGVRDANYEKFDIGKVLERLLLKG
                     TIVVKKAYCDWERYKAFKAPMHEASFELIEIPHLRQSGKNSADIRMVVDALDLCYTKS
                     HVDTFVIISGDSDFSPLVSKLRENAKAVIGVGVKNSTSDLLIANCDEFIYYDDLVRAK
                     RKAPAKKRATKTAAAAAGTPAGAVVEAAPPKRSSDDERRQEAMDLVIETIEALVAERG
                     DDEKIWGSMVKQALRRQKPGFNESFYGFRSFNELLEEAARHALLTLERDDKSGGYRVR
                     LAPAE"
     gene            complement(5289..6080)
                     /locus_tag="VFO53_11685"
     CDS             complement(5289..6080)
                     /locus_tag="VFO53_11685"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET8876982.1"
                     /translation="MDRQRRNWLKTSAAVGAGALASGSLQAQADAKGELLFAHDGSFI
                     PVLGTGQRFQVRRIYCVGRNYAAHSREMGSDPNREPPFFFQKPTDAIQLVPDGTTIDH
                     PYPPVTKNYHHEVELVAAIGKGGRDIPAATANDHVFGYALGLDMTRRDLQQAMGNEKK
                     PWEIGKSFDHSAVVSYLHPASKIGHLAKGAIWLDVNGKRRQSSDLSNMIWTVAEQVSH
                     LSKYYELFPGDIIYCGTPENVGPVVAGDVMHGHIDGLYDIDVKVV"
     gene            complement(6053..7003)
                     /locus_tag="VFO53_11690"
     CDS             complement(6053..7003)
                     /locus_tag="VFO53_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET8876983.1"
                     /translation="MKTGEIRIGISGWRYPPWRGTFYPEGLPQRRELEYASRHVATIE
                     INGSFYSLQRPEFYARWRDETPDNFVFSVKAPRFITHMLKLRNCEQAMANFLASGIAN
                     LGDKLGPILWQLPPTYRFDATVLGPFLASLPRDTDEAAAIARRRNEKVKGRARLAYGA
                     CRPLRHALEVRHETFVDEAFVDLLRREQIALVVADTAGKWPLVEDLTSGFVYARLHGD
                     AVLYVSGYSDAALERWAARFIAWSQGGAPGDARCISRISPAPCAGRDVFCYFDNDAKV
                     MAPRDAQALGHILHAAGAHVVLPGAPAKETSEWIGKDATG"
     gene            7071..7295
                     /locus_tag="VFO53_11695"
     CDS             7071..7295
                     /locus_tag="VFO53_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET8876984.1"
                     /translation="MFALKLTQIGNSVGVILPKEMLARLRVEKGNVIYLTESPDGYRI
                     TPYSEEFEAQMKAARKIMKERRAVLRELAK"
     gene            7292..>7469
                     /locus_tag="VFO53_11700"
     CDS             7292..>7469
                     /locus_tag="VFO53_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HET8876985.1"
                     /translation="MSHVTWLALEVVNAIHEAQLAEHGGSRGTRDTGLLESALARAQN
                     LAAYGVPDIADLAAA"
BASE COUNT         1374 a         2417 c         2532 g         1146 t
ORIGIN      
        1 acgattcgta caccttctcg tgcacgaaga agcggttggc gcacacacac gtctgcccgg
       61 cgttgcggta cttggaaatc atggcgcctt cggcggccgc gtcgagatcg gcgtcgtcga
      121 acacgatgaa cggggcgttg ccgccgagct cgagcgaaat cttcttgatc gtcggtgcca
      181 cctgctgcat cagcaggcgt ccgacccggg tcgagccggt gaacgaaagc ttgcgcacga
      241 tcggattcgc gcacatctcg gcgccgatcg actgcgcgtc gccggtgatc acgttcagca
      301 cgccgggcgg aaagccggcg cgggcggcga gctcggcaag cgcgaatgcc gaaaacggcg
      361 tggcttcggc cggcttgatg accaccgtgc agccggcggc gagcgccggc gacaccttgc
      421 gcgtgatcat cgacgacggg aagttccacg gcgtgatcgc ggcgcacacg ccgaccggct
      481 ccttgacgac gacgagccgc cggtcgctgg ccatcgtcgg gatcacgtcg ccgtagatgc
      541 gtttggcctc ctcggcgaac cattcgacgt acgcggcgcc gatcgtgatc tcgcccttcg
      601 cttcggccag cggcttgccc tgctcggcgg tgaggatgcg cgcgaggtcg tcggcatgcg
      661 cgagcatgag gtcgttccac ttgcgcagaa cgccgctgcg ctccttcgcc gtctttgcac
      721 gccaggtggg gaacgcgcga tgcgccgcat cgatggcgcg cttcgtttcg gcggcacgaa
      781 acaccggcgc cgtaccgatg agcgcgccgc tcgcggggtc gacgaccggc atcgtggcgc
      841 cgtcgtcggc ggcgagccac gcaccgtcga catgacactg ctggcgaagg agggacggat
      901 cggcgagatc gagggcgggt cgttcgagcg tgtcgggcgc catggcgtgc tccttggtga
      961 attcgtaggc gtatggaaaa tcagacagtg ccgcggcgaa cggcgttcgg cccggccggc
     1021 gctgcgcgcg gcctgccggc gccgcgcgac acgagcatga cgcccgcgag caccaggacg
     1081 acgccgatca tctgcagcac gttgatcggc tcgtcgagga gcagcgcgcc gaagccgatc
     1141 gtgaacatcg gtccgagcga gccgacgagc gacgacgtgc tcgcgccgat gcgcctgatc
     1201 gactcggcga tcatccacgt cggcagcacc gtgcagaaga acgccatcgc gagcgcgagc
     1261 agctggatcg actgcggcgc cgcgagtgcc acgaatggac gcgtcagcgc gaagtgcagc
     1321 gagatgaatg cggtggcggc gagcatcgcc cacgcgatga agcgcgacgg accgagacgg
     1381 ccgatgatgc cggtcgcgcc cacgaggtag atcgcgtagc cgaccgcgct ggcgaacacc
     1441 agcgcgctac cgaaggcgat ggcgcgcgga tcaccggtga cgcgcacgtc gtggaacacg
     1501 gcgagcgcga tgccggcata cgagaggacg agggcggcca cggcgcgccg cgtgatcggt
     1561 tgtccgagga acagcgccga cagcacgacg acgatcgtcg ggtagaggaa tagcacaagg
     1621 cgctcgagcg atgcggtgac gtactgcagg ccgatgaagt cgagcaggct cgacaggtaa
     1681 tagccgatga atcccagccc gatcaacagc cgcacgtcgc gcgccgacat gcgggcgatg
     1741 cctggcacgc ggccgctcca ccacgccatc agcaggaaga atggcgccga gtagatcatc
     1801 cgcagcgtca tcaatgcgac gggatcgatg ggcgcccatc gatacgcgag cttgatcagg
     1861 atcgccttga acgaaaatcc gagcacgccc agcactgcga aaagcacgcc cacgctaacg
     1921 cctcgccccg gccggcctgt tcgcgttcat gtccatggcg tcggcaggcg ctaggtccag
     1981 tcgacgaggc ccatccacgc ggtgacgagg atcagcagcc cgaaggcaat ccggtaccac
     2041 gcgaacggca cgaaatcgtg ccggctcacg tagcggatca gccagcgcac gcacaggaaa
     2101 gcggcgacga acgcggcgac gaaaccgatc gcaagaccct gcagatcctg caccgagaag
     2161 agcgcgcggt tgcgcaggaa gtcgtaggcg caggcggcaa gcagtgtcgg gatcgcgaga
     2221 aagaacgaga accgcgtggc cgccggacgc gacagcccga acagcatgcc gccgatgatc
     2281 gtagcacccg agcgcgaggt tccggggatc agcgcgaacg actgcgcgat gccgatcttc
     2341 agtgcgtcga gccagcgcat gtcgtcgacg tcgtcgatcc gcacgcgcga cggcgcgacg
     2401 cgcatccgcc gctcgaccca cagaatgacg agcgcgccgg cgatgaacgc gagcgcgacc
     2461 ggcagtggtg cgaagagatg gcgcttgatc gccttcgcga acagcagccc gagcaccgcg
     2521 gccgggatga atgcgacgat caggttgacg acgaaccgct gcgcgacggg atcgctgcca
     2581 aggccgctga cgacgccgat cagccgtgcg cgaaactccc agcacaccgc gaggatggcg
     2641 ccggcctgga tcacgatctc gaacaccttc gcgcgctcgc cggtgtaacc gaggaacgac
     2701 ccggcgacga tcaggtggcc ggtggacgag acggggagaa actcggtgag accctcgacg
     2761 atgcccagga tcaaggcaac cagagcggac gggtgatcca aaggtgcggg gtcgatttcg
     2821 gcgaggaacg gagcctgcga ttgtcgcata atgcggtcgg ggagaatgcc gcatggacca
     2881 tgaactgcat cagctcttcg ataccgagtc gtcgacgttc acgtacgtgc tcgtcgaccg
     2941 cgcatcgcgc gaagccatcc tcgtcgattc ggtcgagcag cgcttcgagc gcgacatgag
     3001 cctgttgcgc cggctcgcgc tcacgttgcg ctatgtcgtc gaaacgcata cgcacgccga
     3061 tcacgtgacg gccgccgggc ggttgcgcga tgcgaccggt gcgaagacgg tggcgccgtt
     3121 caagtgcggc atttcaccgg ccgacctgca gctcgcgcat ggcgacacgc tgcacttcgg
     3181 cgccggcgag acggtgcacg ccatccatac gcccgggcac acgtcgggca gcacgagcta
     3241 cctgtggcgc ggcaacgtgc tgacgggcga cacgctgctg atcgaaggtt gcgggcgcac
     3301 cgatttccag agcggcgatg cgggttcgct ctacgacagc gtgcacgagc ggctcttcac
     3361 gctgcccgac gacacgcgcg tgtggcccgc acacgattac cgcggcaaca cggtgtcgac
     3421 gatcggccat gaaaagcggc acaacgcgcg cctcgtgggt cgcgaccgtg cgtcgttcat
     3481 cgagctgatg ggcaacctgc agttgccgaa gccgaagctg atcgacgtgg ccgtgccggc
     3541 gaaccgcctg cttggattga cgcacgcagt ctgacgctgc gccgccgccc cgagagcaac
     3601 gccgcctcgc gtaaccgatc agccgccgcg ttcgctgcga agggcttcga tctcgacgcg
     3661 caacgcggcg agctcgttgc gcagcaccgc cacctcgtcg cgaagcaagt cgacgtcctg
     3721 cgcaagcgtc ggcgaatcgc gtggcgcgtc gagcgcgctc gacgcagccg cgcccggtgc
     3781 gggcggcagc gcaggctcgc tcagcaattg catccagcgc gtctcgcgcg tgccgggcga
     3841 gcgcggcaat tgcgccacca gcgcgcccgc ctgacgttcg gcgagctcgt tcaggaatgc
     3901 ctcgacagtg gagatgtcgg cgaagcggtg catgcgctcg gtgccgatcc gcagctcgcc
     3961 cgccgtctgc ggtccacgca gcagcagcgc ggcgagaatg gccaccgact gcgacggcag
     4021 cgcgagcgcg cgctcggcat tgtgcgcata gcgcatgacg cgccccccgc tcgtctccat
     4081 cgccatggac cgtgccttga ggtcgtcgag cgcttgctgc acttcggtgt cggtcagcgt
     4141 gagcaccggg tcgcggctcg acctctgatt gcagccggcg accagtgcat tgagcgtcag
     4201 cgggtacgtg tcgggaaccg tgcgctgctt ttcgacgagc acgccgagca cgcgggcttc
     4261 ggcaacggaa aggcgcggtg gggtcatgat cgaatggtac ttctggtata cagcgaagag
     4321 gaggcccgga attcccgcgg ccgcaaccaa ggcaagatgg ccaacacccc cgaagttacc
     4381 agcatggcgc tgttctgcga tttcgagaac atcgcgctcg gcgtgcgcga cgccaactac
     4441 gagaaattcg acatcggcaa ggtgctcgag cggcttctgc tgaagggcac catcgtcgtc
     4501 aagaaggcgt actgcgactg ggagcgctac aaggcgttca aggcgccgat gcacgaggcg
     4561 tcgttcgagc tgatcgagat cccgcacctt cgccagtcgg gcaagaattc ggccgacatc
     4621 cggatggtcg tcgatgcgct cgacctctgc tacacgaagt cgcacgtcga caccttcgtc
     4681 atcatcagcg gcgattccga tttttcgccg ctcgtgtcga agctccggga aaacgcgaag
     4741 gcggtgatcg gcgtgggcgt caagaactcg acgtcggacc tgctcatcgc caactgcgac
     4801 gagttcatct actacgacga cctcgtgcgc gcgaagcgca aggcgccggc gaaaaagcgc
     4861 gcgacgaaaa cggccgccgc cgccgcggga acacccgccg gcgcggtggt ggaagcggcg
     4921 cccccgaaac gctcgagcga cgacgaacgg cggcaggagg cgatggacct cgtgatcgag
     4981 acgatcgagg cgctggtcgc ggagcgcggc gacgacgaga agatctgggg ctcgatggtt
     5041 aagcaggcgc tgcggcggca gaagcccggg ttcaacgagt cgttctacgg cttccgctcg
     5101 ttcaacgagc tgctcgagga ggcggcgcgg cacgcgctgc tgacgctcga gcgcgacgac
     5161 aagtcgggcg gctaccgcgt gcgcctcgcc ccggcggagt aattggggtc agagacgcat
     5221 ttcgcggcgc aatgcgtcca tacggcgcgg tcgcatcgtg aaatgcgtct ctgaccccga
     5281 atcgcgcgct acaccacctt gacgtcgatg tcgtacagcc cgtcgatgtg cccgtgcatc
     5341 acgtcgccgg cgacgaccgg accgacgttt tccggcgtgc cgcagtagat gatgtcgccg
     5401 ggaaagagct cgtagtactt cgacaggtgc gacacctgct cggcgaccgt ccagatcatg
     5461 ttcgaaaggt cgctcgactg tcgccgcttg ccgttgacgt cgagccagat cgcgcccttc
     5521 gcgaggtggc cgatcttcga cgcgggatgc aggtagctca ccaccgccga gtggtcgaac
     5581 gacttgccga tctcccacgg cttcttctcg ttgcccatcg cctgctgcag gtcgcggcgc
     5641 gtcatgtcga gaccgagcgc gtagccgaac acgtggtcgt tcgcggtggc cgccggaatg
     5701 tcgcggccgc ccttgccgat ggctgcgacg agctcgacct cgtggtgata gtttttggtg
     5761 accggcgggt acgggtgatc gatcgtcgtc ccgtcgggaa cgagctggat cgcatcggtc
     5821 ggcttctgga agaagaacgg cggctcgcga ttgggatcgg agcccatctc gcgcgaatgc
     5881 gccgcgtagt tgcgcccgac gcagtagatg cgccgcacct ggaagcgctg gccggtgccg
     5941 agcaccggaa tgaagcttcc gtcgtgcgcg aacaggagct cgccttttgc gtcggcctgc
     6001 gcctgaagcg aaccgctcgc gagcgcgccc gcgccgaccg ccgccgatgt cttcagccag
     6061 ttgcgtcttt gccgatccat tcgctggttt cctttgctgg tgcgcccggc agcacgacat
     6121 gtgcgccggc cgcgtggagg atgtgcccga gcgcctgcgc gtcgcgcggc gccatcacct
     6181 tggcgtcgtt gtcaaagtag cagaaaacgt cgcgaccggc acaaggcgcc ggcgaaatgc
     6241 gcgaaatgca gcgcgcgtcg ccgggcgcgc cgccctggct ccacgcaatg aaccttgcgg
     6301 cccagcgctc gagcgcagca tcgctgtagc cgctcacgta gagcaccgca tccccatgca
     6361 ggcgcgcata gacgaagccg gacgtgaggt cttcgacgag cggccatttt cccgcggtgt
     6421 cggcgacgac cagtgcaatc tgttcacgcc gcagcaggtc cacgaacgcc tcgtcgacga
     6481 acgtctcgtg ccggacttcc agcgcatgac gcagcggccg gcacgcgccg tatgcaagcc
     6541 gcgcgcgtcc cttgaccttt tcgttgcgcc ggcgcgcgat cgcggccgct tcgtcggtgt
     6601 cgcgcggcag cgacgcaaga aagggtccga gaactgtggc atcgaagcgg taggtcggcg
     6661 gcagctgcca caggatcgga ccgagcttgt cgccgaggtt cgcaatgccc gacgcaagga
     6721 aattcgccat cgcctgctcg caattgcgca gcttcagcat gtgcgtgatg aagcgcggcg
     6781 ccttgacgct gaagacgaaa ttgtccggcg tctcgtcgcg ccagcgggcg tagaactcgg
     6841 gccgctgcag cgagtagaac gagccgttga tctcgatcgt ggcaacgtgt cgcgacgcat
     6901 attcgagctc gcgccgctgc ggcaggcctt ccgggtagaa cgtgccgcgc cacggcggat
     6961 agcgccagcc ggagatgccg atgcggattt cacctgtctt catccaggcg ctatttgccc
     7021 cgtcaatcac gacgccgtag tataacgatt gttataacgg agttgagccc atgtttgcac
     7081 tcaagcttac gcaaatcgga aactcggtcg gcgtcattct gccgaaggaa atgctggcga
     7141 ggctgcgggt agagaagggt aacgtcatct acctgacgga atcacccgac ggctatcgaa
     7201 tcacgccgta cagtgaagaa ttcgaagcgc agatgaaagc cgcgcgcaag atcatgaagg
     7261 agcgtcgcgc tgtgctgcgt gagctcgcga agtgagccac gttacgtggc tcgcgctcga
     7321 agtcgtcaac gccattcatg aagcgcaact ggccgagcac gggggatcgc gaggaacccg
     7381 ggacaccggc ctgcttgaat cggcgctcgc ccgtgctcaa aaccttgcgg cctacggcgt
     7441 gcccgatatc gccgatcttg ccgcagcgt
//
LOCUS       DASSVX010000274        24043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_978603, whole genome shotgun sequence.
ACCESSION   DASSVX010000274 DASSVX010000000
VERSION     DASSVX010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 24043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24043
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978603"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VFO53_11705"
     CDS             complement(<1..1130)
                     /locus_tag="VFO53_11705"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF016553.4"
                     /note="catalyzes the ATP-dependent formation of a
                     phosphodiester at the site of a single strand break in
                     duplex DNA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET8876986.1"
                     /translation="MEAFARLYAELDETNSTRAKLAALTRYFRAAPPDDAAWATYFLA
                     GGRPRRAVAAKLMREAAIDAAALPSWLFDECHLAVGDFAETVAHVLPPPAAVTHAGLA
                     QWMREHVLALRGLPHEAVAQHLRSAWQQLDGPGRLLFGKLCSGNLRVGVSRLLVTRAL
                     AERASIDPKLVAQRLIGYTGADRRPTGDDFRALVAPPAADERAGGRPYPFFLARALVE
                     PAATLGPPGDWLVEWKWDGIRAQLVRRDASTHVWSRGDELVTERFPELQALGDALPDG
                     TVLDGEIVALDGGEIAPFALLSTRIGRKRLSPKVLATAPVAYVAYDILEAGGADLRDL
                     SQLARRARLAEVAARLDAAQLRLSPLVERATWDGYAKLRTQSR"
     gene            complement(1135..2172)
                     /locus_tag="VFO53_11710"
     CDS             complement(1135..2172)
                     /locus_tag="VFO53_11710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HET8876987.1"
                     /translation="MDLIVLRADGLYCPAGDFHIDPWRPVERAVLTHAHADHARAGSA
                     TYVATDASRDVLRVRLPGIRLDTLRYGERVTHNGVAVSLHPAGHVLGSAQVRVEHRGE
                     VWVVSGDYKLAADPTCTPFEPIRCDAYVTESTFGLPIYRWREPAAVIDDIGAWWQRNA
                     DAERTSVLYCYAFGKAQRIAAELQAAGALSTGTLGCHGAMMSLNSAYRASGVSLPAMR
                     HASEIDRNALRRALVLAPPSAAGTPWLRRFGDVADAFASGWMQVRGARRRRALDRGFA
                     LSDHADWPGLNAAIEATGARRVIVTHGRVQAMVRWLTERGIDAEGFATEFAPEGEPES
                     AATPDSATPPG"
     gene            complement(2398..3261)
                     /locus_tag="VFO53_11715"
     CDS             complement(2398..3261)
                     /locus_tag="VFO53_11715"
                     /inference="COORDINATES: protein motif:HMM:NF003840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET8876988.1"
                     /translation="MAAARALGLALALVVAGCVSITAKPRALLLLDDGSVRVQTLSCP
                     QEIERMLATQRHATDSGLDPHAIHMISWNIHKQADPGWQRDLRIFGQRNDLVLLQESV
                     LDPPLRAVIDAEGFRWVMASSFLESGIDIGVLTASRIAPVANCTQRVVEPLLRIPKSA
                     VISWFPLAGRRETLAIVNVHAINFSLSLGAFRAQFDAIGDALEGHEGPLIVAGDLNTW
                     TDARVDAVRALARRLHLTEVRFATDRRSHFFGHELDHIYTRDLATVASLATAVTSSDH
                     NPVAATLSVTR"
     gene            complement(3324..3656)
                     /locus_tag="VFO53_11720"
     CDS             complement(3324..3656)
                     /locus_tag="VFO53_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876989.1"
                     /translation="MAETSAKFETGKSRVQDAGERAQDYLDRAAQTASSGVDRVTDTA
                     RRSVDSAAESAKAGLDWASDKASVLRDRNAALMNAVTDSVSARPMVAIGIAAAIGYLL
                     GRIMRSSD"
     gene            4014..4568
                     /locus_tag="VFO53_11725"
     CDS             4014..4568
                     /locus_tag="VFO53_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876990.1"
                     /translation="MANHIVVGMYESRAEADEARRRLIAEGVRDDRISIEEGAAAGDS
                     PMPPQDRFAMREVGEPLPEDRGVSGFIARMFSGALMDDVNIEKYTEALRDGRCLLAVR
                     AESDEKRQIAASVLEPAGPRVYSLRNAPTAWNEASANDPASIGGVDDDPARPEGLLSD
                     AEGLPAQSDETRFSDTRRGERGNR"
     gene            4657..5082
                     /locus_tag="VFO53_11730"
     CDS             4657..5082
                     /locus_tag="VFO53_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8876991.1"
                     /translation="MPLRSLWSIVAGRPPVEAASITTVVDAARIMKRANIGALLVVDH
                     ARLIGIFTERDALFRLLAEGRDPHATRLADVMTPRPQTIHPDEPFLRALRMMHEGKFR
                     HLPVVEFDRPLGVVSVRDALDEDLHELRVDLEQRETMSE"
     gene            5193..5402
                     /locus_tag="VFO53_11735"
     CDS             5193..5402
                     /locus_tag="VFO53_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HET8876992.1"
                     /translation="MAAHTYKLIELVGTSPTSTDEAIRNAIEKAAATVKHIDWYQVIE
                     SRGHVENGRVSHYQVALKVGFRIED"
     gene            complement(5425..7848)
                     /locus_tag="VFO53_11740"
     CDS             complement(5425..7848)
                     /locus_tag="VFO53_11740"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET8876993.1"
                     /translation="MITQLGKYEIRRELARGAMGVVYEGYDPLIKRKVALKTIRPDQL
                     DSERNGDLLARFRREAQAAGRLNHPNIVAVYDFDEDAGTAFIAMEFVEGRELKECFTA
                     DERFRMPDIERIMTSILDALDYSHRQGVVHRDIKPANIFLLGDGSVKVADFGIAHIES
                     SNLTQVGTVVGTPNYMSPEQIMGLPVDGRTDLFAAGVILYQFLTGERPFAGSTTTTMQ
                     KVLKEEPLPPSTLNVQLPPAIDAVVREALAKRPDDRYASARDFAQALRTAIRATEPVR
                     HDPDATMPNLAPLDPPATAMASATAATMQPHARPLSVASPSAPASIGPPVSGASRTNQ
                     RAAMGIVAAIAVVGIAALAATWWLFPRPSTPDRSQAKAAGPIPTAAAPAPVKAPATPM
                     PVAATAPAPALATPVAGMPAPAGRLLVTAVGLADPGNARYASDPSLLQADVRADARSQ
                     LVAKALLLMLDRKSFASNYDLLQSRLLAQSESFVGDVVQQSAPQVGKDGLMSVTTQAV
                     VDVDAIRRALDRMTREERVQLIRANGDPRIAVRIAVRDEAAPAASAAPSPLAENVVKE
                     RIASFGFRTWNEGAGAPGAADADFVVSGEASVRRLSTRLAASGLVITKYALASWTVKC
                     TDKATGEEIYFNTTLPNASGSYASEEAALRAIGTGVADAFSRDFFLTHVPVRGRKVAL
                     VVAGLSDDTAQALLARELLGLPGVIDARAGPPANPRTYDVEIARGGGNEAIVRDILRP
                     LNAKLGDACFSTGASEGARISIAFEARCAEGLRARLESNPPAGLYDAPPARQKAVVTN
                     PETLRKLMV"
     gene            complement(7868..8704)
                     /locus_tag="VFO53_11745"
     CDS             complement(7868..8704)
                     /locus_tag="VFO53_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876994.1"
                     /translation="MNGRLPIHVVSPLLGAALAFASVASLAQPTFGNPSPTAGSETSQ
                     RANESADQALYKPVEYANRNRQGPAVIVIPGEIKSSNATFLQKFTSNNIADFGELELS
                     AANFKVLERANLGPLLKEFELAYNLGDPQEARKFLGMGKLKSTKYVVKFDILKTEQVA
                     AAQQGFDGRALGNIAGALGAFSGSWGGARAGAVGQAAVGSVHTGESSGVWIIGMRYKI
                     INAETTEQLAQGYTEEKMEVGAKSSSVLGVSQSQQGGVSLDTMVQRLVQKSVWEIDSK
                     YK"
     gene            complement(8762..9427)
                     /locus_tag="VFO53_11750"
     CDS             complement(8762..9427)
                     /locus_tag="VFO53_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8876995.1"
                     /translation="MRRAQASSTLLLLGALVAGAAVGQGFRFSQPDDTAAREAAARDE
                     RIAQDLSQPCRAGLLNRKIMVIIGERQSNGFVEAHQQNYGPHYQAINKRLQALGLRTY
                     TPDEIRRQIAQAEIDAYFRNDPDAALAASRRLGASFVLRGLISSQATRNPMMAVNQVA
                     VNMGFTLTGSNGRVISDTEASASSYAGADVRRMAQTLVDEKADEVVAKLYGDYCRNAV
                     PRK"
     gene            9548..13891
                     /locus_tag="VFO53_11755"
     CDS             9548..13891
                     /locus_tag="VFO53_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET8876996.1"
                     /translation="MDRLFHPAVAAWLAKTFAAPTEAQARAWPAIHAGRDVLIAAPTG
                     SGKTLAAFLAAIDALVRQGVEHPLPDETFVVYVSPLKALSNDIRRNLEAPLAGIRSEL
                     ASLGSPDVDIRTLVRTGDTPQHERARMRKRPPHIVVTTPESLYVLLGSESGRAMLATT
                     RTVIVDEIHALASNKRGSHLALSLARLQALATHRVTRIGLSATQRPIEEVARFLVGRN
                     AADAVIVDTGHVRPRDLALEVPPSPLEAVMSNEVWLQVYARLADLVARHRTTLVFVNT
                     RRMSERVTRHLSELLGAAHVMAHHGSMAKELRLAAEARLKCGELKALVATASLELGID
                     IGDVDLVCQIGSPRSISTFLQRAGRASHTVHGVPKARLFPLSRDELVECTALLDAVRR
                     GELDRVTIPGKPLDVLAQQIVAEVAAHDWPERGLYDMLTGAYPYATLEYDDYLDVVRT
                     LSDGYTTRQGQRGAWLHRDAVNGVLRARRGARMTALTSGGAIPDTADYDVILEPAGHM
                     VGTVNEDFAVESLAGDVFQLGNTSYRIRRIEAGRVRVEDAQGQAPNIPFWLGEAPARS
                     DELSFAVSRLRSDLFASARSHAGPADAASALAALDASGMEPAAASQLVDYLANARSVL
                     GTLPTQDRIVLERFFDESGGMQLVIHAPFGSRINRAWGLALRKRFCVRFNFELQAAAT
                     EDAIVLSLSTSHSFALADVAHYLHSNTVRAVLVQAMLAAPMFTARWRWIANTSLALPR
                     FRGGKKVPAPLQRMRADDLMAAVFPDQIACAENIVGEREIPDHPLVRQVVHDCLHEAM
                     DIDGLERLLRRIERGDVEIVARDLPAPSPLAMEILNARPYAYLDDAPLEERRTQAVMA
                     RRWTDDDASGKLGELDAAAIERVRIEAWPEASRPDEMHAALLALGFVTDAEIRAHDGW
                     REFLEALAHDRRATAVALAAEHRVFAAAERLPQFDALYAGAAHAPPIGAPAEYAERAW
                     SRDEALTELVRSRLQALGPVTASAIADSFALPVGEIEGALARLAGEGVAMNGSYTPDA
                     TRPEWCDRTLLARIHRYTIKRLRQEIEPVSTQDFVRFLFRWQHVAPDDMRQGPDALDA
                     IIGQLQGFEAPAAAWESEILPARLSDYEFTWLDDLCLSGRAVWARLTPSSGGATNVIR
                     TTPVTLLPRRSAATWIAAAPSGDEDAVAASARAQAVAAFLRANGASFYDEIVDGVHLL
                     RTQVEDALAELVALGSVTSDSFAGLRALLTPSSKRKRLGGGKRHRRTALFGIEDAGRW
                     SLARRAPRPAQDTAAHGWTRSDPAHAIVEHVAHALLRRYGVVSWRILEREAAWLPPWR
                     ELSRVLRRLEARGDIRGGRFVASITGEQFALPEALALLRETRRAPAAAHFVSISASDP
                     LNVVGTLLPGARIPAITGNRVLYRDGVAVAALVAGEMRMLVPLEQADARAAEHALIRH
                     PPGAAPFAYLH"
     gene            14078..15241
                     /gene="opgC"
                     /locus_tag="VFO53_11760"
     CDS             14078..15241
                     /gene="opgC"
                     /locus_tag="VFO53_11760"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HET8876997.1"
                     /translation="MERQTELDWLRGLMLVLMTMTHLPTWFSAHAGQPFGFVSAAEGF
                     VFLSAFLVGSVYAHKARTRGYAVMRRALWGRALKVYLAHIGLLLFLLLVLVPIAVSHN
                     AQAITGLASYYLERPHLALVSGLILAYNPPLLDILPMYVLFLALSPLALRLGTQRGWA
                     LLLALSAVLWLFAQYDGGLHVYKSLAALVGWPVPYGATGAFSFMAWQLLWLVGLRLGA
                     VNGVADLPRSPIGDRRWPGFGMLAAMSVAAVFFTWRHVVGQAPFGSDAMLNALFDKWH
                     LGPLRLLNFAVLALIVVHVRPVLVEWAAHSSIATLGRASLTVFSAHLLICLALLTIIG
                     EAARPHLSVFETLLLLGTLAALYGVAQATLAAPRVVRAGSRALAARLSARGAR"
     gene            complement(15219..15902)
                     /locus_tag="VFO53_11765"
     CDS             complement(15219..15902)
                     /locus_tag="VFO53_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET8876998.1"
                     /translation="MNGKLLLAAALTVFASIGTAGAAALPDAPIPGSRWDAAFAAFAA
                     DDEAHPHPAGGVLFVGSSSIRLWSDLESQFHDLPVVIKRGFGGSQLSDCVKHLSRLVL
                     RYRPRTVLVYAGDNDLATGSAPSEVLHRFTAFADGVHQALPDTEIFYISIKPSPARVA
                     LLPQIRATNALIRDYADREGKVDYIDVFTPMLDASGRPRPELFRADSLHLNTDGYALW
                     KRVIAPHVH"
     gene            complement(16020..16523)
                     /locus_tag="VFO53_11770"
     CDS             complement(16020..16523)
                     /locus_tag="VFO53_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET8876999.1"
                     /translation="MNDAYPIDGGCTCRNVRYRMTTKPLFVHCCHCRWCQRETGSAFV
                     LNALIESDRVVVLQGDPVTVATPSNSGRGQKIVRCPDCQVALWSHYAGAGDLFKFVRV
                     GTLDEPDRLPPDIHIFTLSKQPWVILPAGTPAMPEYYDSKRYWPAESLARRDVVRSAA
                     ARPPSPA"
     gene            complement(16520..16927)
                     /locus_tag="VFO53_11775"
     CDS             complement(16520..16927)
                     /locus_tag="VFO53_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8877000.1"
                     /translation="MIRIRDIDHLVLRVVDVEAMLAFYRDVLGCSIERRRDELGLVQL
                     RAGRSMIDLVPVDGKLGRAGGAAPGSEGRNLDHFCLRVEPFDEPAIRRHLQAAGIAAG
                     EAVPRYGAEGEGPSIYVTDPEGNMVELKGPPAS"
     gene            complement(16953..17348)
                     /locus_tag="VFO53_11780"
     CDS             complement(16953..17348)
                     /locus_tag="VFO53_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8877001.1"
                     /translation="MNALNIDRIDHVVMTVGDIEATCDFYSRVLGMRVVTFAGGRTAL
                     AFGRQKINLHLAGREFEPKAAHPSSGSLDLCLIATGTLDEVIAQLEACAVPIIEGPVA
                     KTGATGPIRSVYFRDPDANLIEVSIYENA"
     gene            complement(17345..18097)
                     /locus_tag="VFO53_11785"
     CDS             complement(17345..18097)
                     /locus_tag="VFO53_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HET8877002.1"
                     /translation="MQTIGLLGGMSWESTLPYYRTINEAVRDALGGLHSASIVLNSVD
                     FHEIERLQDAGDWARAGEILADAARSVEAGGADFLVLCTNTMHKVAPAITRAVTIPLL
                     HIADPTAAAIVDAGLSSIGLLGTRFTMEQDFYRDRLAAHGITAIVPDDDERECIHRVI
                     YDELCRGVVRDASRVRYRSIIGRLVERGARGIVLGCTEIAMLIDAGDVAVPLFDTALL
                     HARAAAMRALTGESRRLAHATAAPRRHAQNIA"
     gene            complement(18100..19020)
                     /locus_tag="VFO53_11790"
     CDS             complement(18100..19020)
                     /locus_tag="VFO53_11790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8877003.1"
                     /translation="MIGDTVSLVVLTFNRRNEVLRTLSRLAEIDASVPIVVVDNASTD
                     GTAEALASLCPRVRIVRLPSNAGAAGRNAGVRVCTTPYVAFCDDDTWWLPESIALAAA
                     ALDAHPALAAVTARVLVGERRRDDPTNAVMAASPFPNRLGVRGSELLGMLAGACMVRR
                     AAFLDAGGYHPRLFLGREEALLAIDLMTAGWHMAYVPDAVVCHFPSCVRDTARRSRMS
                     ARNALWIAWLRRPMRTAWRATGDWWAASRRDGVALRNARDALRGLAWVLRERRVVPAH
                     VESALQTLDALQAVNEEYRPMRQFPKVHPG"
     gene            complement(19017..19706)
                     /locus_tag="VFO53_11795"
     CDS             complement(19017..19706)
                     /locus_tag="VFO53_11795"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET8877004.1"
                     /translation="MRDASRRLFISPHLDDAVFACGEWIASCASPLVVTIFAGFPPAG
                     ATLTPWDRECGFGEGDDVIALRRHEDRTALDSLGAAAIQLDFRDDQYGEPRPTRVITD
                     ALAELVAHEAPSAVHVPLGLFHADHRRSSDASLALFDRFATRAWHVYEDAIYRCIPGA
                     VDERTRVLAERGYVLQRRSPAMARDAAARKRHAVGCYRSQLRALRTRNAHGDVFAPER
                     HSALSRSAGSR"
     gene            complement(19715..20242)
                     /locus_tag="VFO53_11800"
     CDS             complement(19715..20242)
                     /locus_tag="VFO53_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET8877005.1"
                     /translation="MHVGRESAVCIGLISDTHGLLRPEALAALRGSDVIVHAGDIGDG
                     SLLERLAAIAPVCAVRGNNDVGAPGSGLPQTQRLDVGGKCILVIHDLAELTAEPVAAG
                     IDAVVSGHSHQPTIERRDGVLYVNPGSAGPRRFRLPVSVGRLTIAADRIEAELLPLAV
                     AAAPATARAVTRKRR"
     gene            complement(20248..20649)
                     /locus_tag="VFO53_11805"
     CDS             complement(20248..20649)
                     /locus_tag="VFO53_11805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HET8877006.1"
                     /translation="MEDDDSAIHTLLRTWHEATAAGDVARILPLMAEDVVFLGAGRAP
                     MRGRRAFEQGLRTLLTHSRIDSRGEVREIVVSGDLAYCWSELSVKVTPIGGGAATHLA
                     GPALSILRKRDGAWAIVRDANMLAPRAARMP"
     gene            complement(20653..21057)
                     /locus_tag="VFO53_11810"
     CDS             complement(20653..21057)
                     /locus_tag="VFO53_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HET8877007.1"
                     /translation="MAAGGMNVPAAPAMLARFAADSVLLLHLCFILFVVLGGLLVVRW
                     HALMPAHIAAVAWAVFVEATGKICPLTFAENRLRDAAGLAGYGGSFVEHYLLGVIYPA
                     GLTRPGQYLLAFLVVAVNATIYLTLLRSLRER"
     gene            complement(21039..21542)
                     /locus_tag="VFO53_11815"
     CDS             complement(21039..21542)
                     /locus_tag="VFO53_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET8877008.1"
                     /translation="MPMSDYIRGLRAMIGTTVLEVPTVSVLAFDDANRVLLVRHAEGN
                     DWTTPGGMIEPYELPADAAVREMWEETGLYVELTRLIGVFGGRVCTSTYANGDRLAWV
                     SSVFAGRPVRGELRPDGEETLEVRYFTATEASQLRCKPHVAMFLEAGWAARQHGHFQR
                     PTWQPAA"
     gene            complement(21576..22190)
                     /locus_tag="VFO53_11820"
     CDS             complement(21576..22190)
                     /locus_tag="VFO53_11820"
                     /inference="COORDINATES: protein motif:HMM:NF045184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877009.1"
                     /translation="MPNPAQTGAMAPHAEHGEIDRRSAHLPGRDERLAMQLQRLGAAR
                     FAHVNGTLEQHLHATMRLLRRWGNREPVCMAGLYHAVYGTGGIAGRLAGLDARTAIAE
                     VIGNEAEALAYLYGACDRERFHPRIGTPAQSTFVDRFTNGEYEIGEAELRAFCEMTVA
                     NELELAQASASFRVRHRAELRRLFARMSGLISGAAHDAAAAALG"
     gene            complement(22213..22914)
                     /locus_tag="VFO53_11825"
     CDS             complement(22213..22914)
                     /locus_tag="VFO53_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET8877010.1"
                     /translation="MWSARRHRERHRTNRIGWLRAAVLGANDGIVSTASLVAGVAAAH
                     ATHADIVVAAVAGLVAGAMSMAAGEYVSVHSQADTEHADLERERKELTSDLQGERNEL
                     TAIYVRRGLDQALARQVADQLMAHDALAAHARDELGISDTIRPRPIQAALASAASFAV
                     GAALPLVVTAVAPEAHLIPVVCATSLVFLAILGGVAARAGGARIGIGAARVTFWGALA
                     MAVTAGVGALLGTTP"
     gene            complement(22957..23265)
                     /locus_tag="VFO53_11830"
     CDS             complement(22957..23265)
                     /locus_tag="VFO53_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HET8877011.1"
                     /translation="MLELRPTCENCNKALPADALDARICSYECTFCAACVEAVLQNVC
                     PNCGGGFTPRPIRPARNWKGDNFLGTDAAGIRVRHRPVDPQAHARFAAAIKSIPPHER
                     "
     gene            complement(23357..23914)
                     /locus_tag="VFO53_11835"
     CDS             complement(23357..23914)
                     /locus_tag="VFO53_11835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HET8877012.1"
                     /translation="MTTQRELMLAGQPYDPLDPELTAARERARELCRALNLTRESPEQ
                     QRRQLLRALFAAGGDSVGMQPPFHCDYGSNIYLGTRVFFNFNCVVLDVCDVRIGDYTL
                     FGPGVQILTPLHPLDARARRQQEFGKPVTIGADVWVGGGALILAGVNIGEGAVIGAGS
                     VVTRDVPDGALAAGNPCRVIRNVAS"
BASE COUNT         3714 a         8117 c         8303 g         3909 t
ORIGIN      
        1 cgcgattggg tgcgcagctt tgcgtagcca tcccaggtcg cgcgttcgac caacggcgaa
       61 agccgcaact gcgcggcgtc gagccgggcc gcgacctcgg cgagacgcgc gcgacgcgcg
      121 agctgggaaa ggtcgcgcag atcggcgccg ccggcctcga ggatgtcgta cgcgacgtag
      181 gccaccggcg ccgtggcgag caccttcggc gacagccgct tgcggccgat ccgcgtcgag
      241 agcagcgcaa acggcgcgat ctcgccgccg tcgagcgcga cgatttctcc gtcgaggacg
      301 gtgccgtccg gcagcgcgtc gccgagcgcc tgcaattcgg gaaagcgctc ggtgacgagc
      361 tcgtcgccgc gtgaccatac gtgcgtgctt gcgtcgcggc gcacgagttg cgcgcgaatg
      421 ccgtcccatt tccattcgac gagccagtcg ccgggcggac ccagcgttgc agcaggctcg
      481 acgagggctc gcgccaggaa gaacgggtac ggacgcccac ccgcgcgttc atccgcagcg
      541 ggtggcgcga ccagtgcacg gaagtcgtcc ccggtcggcc tgcgatcggc gccggtataa
      601 ccgatcagtc gctgcgcaac gagcttcggg tcgatggatg cgcgctcggc cagcgcacgc
      661 gtgaccagca accgcgatac gccgacgcga aggttgcccg agcagagctt gccgaagagc
      721 aggcggcccg gcccgtcgag ctgctgccag gcacttcgca ggtgttgcgc caccgcctcg
      781 tgcggcaagc cgcgcagcgc aagcacgtgc tcgcgcatcc actgcgcgag ccccgcgtgc
      841 gtcacggcgg ccggcggcgg cagcacatgc gcaaccgtct cggcgaaatc gcccaccgcg
      901 agatggcatt cgtcgaatag ccacgatggc aaagccgccg cgtcgatcgc cgcctcgcgc
      961 atcagcttcg ccgcgaccgc tcgacgtggc cgtccgccgg ccaggaaata cgtggcccat
     1021 gccgcgtcgt cgggcggtgc cgcacggaaa tagcgcgtca gcgcggcaag cttcgcgcgt
     1081 gtcgaattcg tttcgtcgag ctcggcatag aggcgggcga acgcttccat tgcatcagcc
     1141 cggcggcgtc gcgctgtcgg gcgttgccgc cgattccggt tcgccctcgg gggcgaattc
     1201 ggtcgcgaat ccttcggcgt cgatgccgcg ctcggtcagc cagcgcacca tcgcctgcac
     1261 cctgccgtgc gtcacaatga cgcgtcgcgc gccggtcgcc tcgatcgcgg cgttgagacc
     1321 gggccagtcg gcatggtcgg aaagggcgaa gccgcgatcg agcgcgcgcc gccgacgggc
     1381 gccgcgcacc tgcatccatc ccgacgcgaa cgcatcggcg acgtcgccga agcggcgaag
     1441 ccacggcgtg ccggccgcgg aaggcggtgc caggacgagg gcgcggcgca gcgcgttgcg
     1501 gtcgatctcg cttgcgtgcc gcatcgcggg cagcgatacg cccgacgcgc ggtacgcgct
     1561 gttcagcgac atcatcgcgc cgtggcatcc gagcgtgccg gtggaaagcg cacctgcagc
     1621 ctgcaactcg gcggcgatcc gctgcgcctt cccgaacgca tagcagtaga ggacgctggt
     1681 gcgctccgcg tcggcgttgc gttgccacca cgccccgatg tcgtcgatca ccgcggccgg
     1741 ctcgcgccag cggtagatcg gcagtccgaa cgtcgactcg gtgacgtacg cgtcgcagcg
     1801 gatgggctcg aacggcgtgc aggtcggatc ggccgcgagc ttgtagtcgc cggacaccac
     1861 ccagacttcg ccgcgatgct cgacgcgcac ctgggccgag ccgagcacgt gacccgcggg
     1921 atgaagcgaa acggcgacgc cgttgtgcgt gacgcgctcg ccgtagcgga gcgtgtcgag
     1981 acgaattccg ggcagccgta cgcgcagcac gtcgcgcgac gcatcggttg caacgtaggt
     2041 cgcgctgccg gcgcgcgcat ggtcggcgtg cgcgtgcgtc aggaccgccc gttcgaccgg
     2101 acgccacgga tcgatatgaa agtcgccggc ggggcagtag agcccgtcgg cgcgcagcac
     2161 gatgagatcc atggtttcgc ggcgggtggt cctacatgaa ttcgagcgcg atgccgatac
     2221 atcgcggtgg ccgcaatccc gatactgcgt tcgtacgggc accgatggga aggcgcccgc
     2281 gtcgtcgaag aacgccgatc atgcgacaac aatggatgaa tcgaaccacg ctcgacgtgt
     2341 tgctcgtggt gctgctgctt gcgtgggtgc tgtaggcggt gccttccggc tcgcgcgtca
     2401 gcgcgtgaca ctcaaggtgg ccgcaaccgg attgtgatcc gatgacgtga cggcggtcgc
     2461 gagcgacgca accgtcgcga ggtcgcgcgt gtagatgtga tcgagctcat ggccgaagaa
     2521 gtggctgcgc cggtccgtcg cgaaccgcac ttcggtgagg tgcaggcgcc tcgccagcgc
     2581 gcgcacggcg tccacgcggg cgtcggtcca cgtattgaga tcgcccgcga cgatcagcgg
     2641 cccctcgtgg ccctcgagcg catcgccgat ggcatcgaac tgcgcgcgga atgcgcccag
     2701 cgaaagcgag aagttgatcg cgtgcacatt gacgatggcg agcgtttcgc ggcgaccggc
     2761 aagcgggaac cacgagatga cggccgattt cggaatgcga agcagcggct cgacgacccg
     2821 ttgcgtgcag ttggccaccg gtgcgatcct cgacgcagtg agaacgccga tatcgatgcc
     2881 cgattcgagg aacgagcttg ccatcaccca gcgaaagcct tcggcgtcga tgacagcgcg
     2941 aagcggcgga tcgagcaccg attcctgcag cagcacgagg tcgttgcgct ggccgaaaat
     3001 gcgcaggtcg cgctgccagc cgggatccgc ctgcttgtgg atattccagc tgatcatgtg
     3061 gatcgcatgc ggatcgagac cactgtccgt ggcgtggcgt tgcgttgcga gcatacgctc
     3121 gatctcctgc gggcacgaca gcgtctgcac acgcaccgag ccgtcgtcga gcagcagcag
     3181 cgcgcgcggc ttcgccgtga tcgaaacgca gccggccacg acgagcgcca gggcgaggcc
     3241 cagcgcccgc gcggccgcca tgcggctggc gtgaaaacgg cggccgtgga gcgcgcggcc
     3301 gccgcgtcgg gtcatgcgac ggatcaatcg ctgctgcgca tgatgcgccc gaggagatag
     3361 ccgatcgccg cggcgatgcc gatcgccacc atcggccgcg cgctcaccga gtcggtgacc
     3421 gcattcatca gcgccgcgtt gcggtcgcgc aataccgacg ccttgtcgga tgcccagtcg
     3481 aggcccgcct tggccgactc ggccgccgag tcgacgcttc ggcgcgcggt gtccgtcacc
     3541 ctgtccacgc cggacgatgc cgtttgcgcg gcccgatcga ggtaatcctg ggcacgctcg
     3601 cccgcgtcct gcacgcgcga tttgcccgtt tcaaatttcg cactggtttc tgccatggtg
     3661 ttctccgtag gtcgccgtcg cggcccgtca aggatgcggg cgtacgccgg ttgttgatat
     3721 cggacgcaat cggcatttca cgtaggcgtg tgaccgacac tcattcgaac tcctcttgtc
     3781 tcaggttggc gacgcccgat tcgcttgaat gggcgctgct tgtccggatt gcgtagcacc
     3841 ccgcatgcca cgcgttgacg ccgcggttcg cgcaagcagc gctgcgaatt gagcgtaccg
     3901 tgcgcgcgcg cgatggcaaa tgtgcccgct tcgcgcgcaa tttttcccat tgacgtgaga
     3961 catctgccgc acgggcgctt cgaacttcga cgacgcatcg cattccaacg cgcatggcta
     4021 accacatcgt tgtcgggatg tacgagtcgc gcgccgaagc ggacgaagcg cgccgacggc
     4081 tgatcgccga aggcgtgcgc gacgatcgaa tcagcatcga agagggcgct gccgcgggtg
     4141 attcgccgat gccgccgcag gatcgcttcg cgatgcgcga agtcggcgag ccgctgccgg
     4201 aggaccgcgg tgtgtcgggt ttcattgcgc ggatgttcag cggcgcgctg atggacgacg
     4261 tgaatatcga gaaatacacg gaggcgctgc gcgacggccg ctgtcttctt gcggtgcgtg
     4321 ccgagtccga cgaaaagcgg cagatcgccg cgtcggtcct cgaacccgcc ggtccgcgcg
     4381 tctattcgct gcggaacgcg cccaccgcgt ggaacgaggc gagcgcgaac gatcccgcga
     4441 gcatcggcgg cgttgacgac gacccggcgc gtcccgaggg attgctgagc gacgccgagg
     4501 ggttgcctgc gcagtccgac gagacgcgct tttccgatac ccgccgcggc gagcgcggca
     4561 atcgctgacg ccgcggcctt tcatcgcgct gcgtggccga gtatcctcgc atcgagcgcc
     4621 gcgtgtcgcc ggcgcaacga ctgacgggga ctttacatgc ctcttcgctc attgtggtcc
     4681 atcgtcgccg ggcgtccgcc cgtggaagcc gcgtcgatca ccaccgtcgt cgatgcggcg
     4741 cgcatcatga agcgcgcgaa catcggtgcg ctgctcgtcg tcgatcacgc gcggctgatc
     4801 ggcatcttca ccgagcgcga cgcgctgttc cggctgctcg ccgaaggccg cgatccgcac
     4861 gcgacccgcc tcgccgacgt gatgacgccg cgcccgcaga ccattcatcc cgacgagccg
     4921 ttcctgcgcg cgctgcgcat gatgcacgag ggcaagttca ggcacctgcc ggtcgtcgag
     4981 ttcgatcggc cgcttggcgt cgtttcggtg cgtgacgcgc tcgacgagga ccttcacgag
     5041 ctgcgcgtcg acctcgagca gcgcgagacg atgagcgagt agccgcgccg aaaccagtcg
     5101 gccgcgcgcg tcagggcgcg ctagaatgac cgtcggcgtt ggctgccgcc gatgcccggc
     5161 atccgtctcg cacaaccaga gagaggactt ccatggccgc gcacacctac aagctgatcg
     5221 agctcgtcgg cacgtcgccg acaagcaccg acgaagcgat ccgcaacgcg atcgagaaag
     5281 ccgcggcgac ggtcaagcac atcgactggt accaggtgat cgagtcgcgc ggccacgtcg
     5341 agaacggacg cgtgtcccac tatcaggtgg cgctcaaggt gggctttcgc atcgaggact
     5401 gatcgtcgtt tcccgaacta cgccctacac catgagtttg cgcagcgttt cgggattggt
     5461 cacgacggcc ttctggcgcg ccggaggcgc gtcgtaaagg cccgccggcg gattggactc
     5521 gaggcgcgca cgcagccctt cggcacaccg ggcctcgaac gcaatggaaa ttcgggcacc
     5581 ttcgctggcg ccggtgctga agcaggcgtc gccgagtttc gcattgagcg gacgcaggat
     5641 atcgcggacg atggcttcgt tgccgccgcc gcgcgcgatt tcgacgtcgt aggttctcgg
     5701 gttggcgggc gggcccgcgc gggcgtcgat cacgccgggc agccccagca gctcgcgtgc
     5761 gagcaacgcc tgcgccgtgt cgtcggataa cccggcgacg acgagggcca ccttgcgtcc
     5821 gcgcaccggc acgtgcgtca ggaagaagtc gcggctgaat gcgtcggcca cgccggtgcc
     5881 gatcgcgcgc agcgccgctt cctcgctggc gtaactgccc gacgcgttcg gcagcgtcgt
     5941 gttgaaatag atttcctcgc ccgtcgcctt gtcggtgcat ttgaccgtcc acgacgcgag
     6001 cgcgtatttc gtgatgacga gtcccgatgc ggcgagccgg gtcgacagcc tgcgcacgct
     6061 cgcctcgccg ctcacgacga agtcggcgtc ggcagccccc ggtgcgccgg cgccttcgtt
     6121 ccacgtgcga aaaccgaacg acgcgatacg ctccttgacg acgttctccg cgagagggga
     6181 gggcgccgca ctggctgcgg gcgccgcttc gtcgcgcacc gcgatgcgca ccgcgatgcg
     6241 cggatcgccg tttgcacgga tcagttgcac gcgctcctcg cgcgtcatcc gatcgagcgc
     6301 gcggcggatc gcgtcgacgt cgacgacggc ctgcgtcgtc accgacatga gcccgtcctt
     6361 gccgacctgc ggtgcgctct gctgcacgac gtcgccgacg aaactctcgc tttgcgcgag
     6421 cagccgcgac tgcagcaggt cgtagttgct ggcaaacgat ttgcggtcga gcatcagcag
     6481 cagcgccttc gcgacgagct ggctgcgcgc gtcggcgcga acgtcggcct gcagcagcga
     6541 cgggtcgctc gcgtagcgcg cgttgccggg gtcggcgagg ccgaccgcgg tgacgagaag
     6601 cctgccggct ggcgcgggca tcccggccac gggcgtggct agtgcgggtg cgggcgccgt
     6661 cgcggcaacg ggcatcggcg ttgccggcgc tttcacgggc gcgggtgctg cagcggtcgg
     6721 aatcggccct gccgcctttg cctgcgagcg atcgggcgtc gaagggcgcg ggaacaacca
     6781 ccatgttgcg gccaatgcgg cgatgccgac gacggcaatc gccgcgacga tgcccatcgc
     6841 ggcgcgctgg ttcgtgcgcg acgccccgct cacgggcggg ccgatgcttg ccggcgcaga
     6901 cggcgaagca acggacaatg ggcgtgcgtg cggctgcatc gttgctgcgg tggcgctcgc
     6961 cattgcggtg gccggcgggt cgagcggggc gaggttcggc atcgtcgcgt ccggatcgtg
     7021 ccgaacgggc tcggtggcgc gaatggcggt ccgcaacgcc tgcgcgaagt cgcgtgcgct
     7081 cgcgtagcgg tcgtcgggtc gcttggcgag cgcctcgcgc acgacggcat cgatggcagg
     7141 cggcagctgc acattgagcg tcgacggcgg cagcggctcc tccttcagca ccttctgcat
     7201 cgtggtcgtc gtcgatcccg cgaacggccg ctcaccggtg aggaactgat agaggatgac
     7261 gccggcagcg aacagatcgg tgcgtccgtc gaccggcagg cccatgatct gctcgggcga
     7321 catgtagttc ggcgtgccga cgacggttcc gacctgcgtc aggttcgacg attcgatgtg
     7381 cgcgatgccg aagtcggcca ccttcacgct gccgtcgccg agcaggaaga tgttcgcggg
     7441 cttgatgtcg cgatgcacca cgccctggcg atgcgagtag tcgagcgcat cgaggatcga
     7501 cgtcatgatc cgctcgatgt ccggcatgcg gaagcgctcg tcggccgtga agcattcctt
     7561 gagctcgcgg ccctcgacga attccatcgc gatgaatgcc gtacccgcgt cctcgtcgaa
     7621 atcgtagacc gcgacgatgt tcggatggtt gagccggccc gccgcctgcg cctcgcgacg
     7681 aaagcgcgcg aggagatcgc cgttgcgctc gctgtcgagc tgatcgggcc ggatcgtctt
     7741 cagcgcgacc ttgcgcttga tcagcgggtc gtagccctcg tagacgacgc ccatcgcgcc
     7801 gcgagcgagc tcgcggcgaa tctcgtactt tccaagctgg gtgatcacgc tacggtcacc
     7861 gcctgcgcta tttgtacttg ctgtcgatct cccacaccga cttctgcacc aggcgctgca
     7921 ccatcgtgtc gagagagacg ccgccctgct gcgattgcga aacgccgagc accgacgacg
     7981 acttggcgcc gacctccatc ttctcctcgg tatagccctg cgcgagctgc tcggtggttt
     8041 cggcgttgat gatcttgtag cgcatgccga tgatccacac gccgctcgat tcgccggtgt
     8101 gcaccgatcc gacggccgcc tgtccgaccg cccccgcgcg cgcaccgccc cacgagccgc
     8161 tgaacgcgcc gagcgccccg gcgatgttgc cgagcgcgcg cccgtcgaaa ccctgctgtg
     8221 ccgcggccac ctgctcggtt ttcaggatgt cgaacttgac gacgtatttc gtgcttttga
     8281 gcttgcccat gccgaggaac ttgcgcgcct cctgcggatc accgaggttg taggcaagct
     8341 cgaattcctt cagcagcggt ccgaggttcg cgcgctcgag caccttgaag ttggcggccg
     8401 agagctcgag ctcgccgaag tcggcgatgt tgttcgacgt gaacttctgc aggaaggtcg
     8461 cgttgctgct cttgatctcg cccgggatga cgatcaccgc cgggccctgc cggttgcggt
     8521 tcgcgtactc gaccggcttg tacagcgcct ggtcggccga ctcgttcgcg cgctgcgacg
     8581 tttcgctgcc ggcagtgggc gacggattgc cgaacgtggg ttgcgcgagg cttgcgacgc
     8641 tcgcgaatgc aagcgctgcg ccgagcaacg gggaaacgac gtggatcgga aggcggccgt
     8701 tcatggaaat gctcctcttc ggttcgaatc gtgtttcatc gagcgctgac agggcaccgc
     8761 gtcattttcg agggacggcg ttgcggcagt agtcgccgta gagcttcgcg acaacctcgt
     8821 ccgccttttc gtcgacgagc gtctgcgcca tgcgccgcac gtcggcgccg gcataggagc
     8881 tcgcacttgc ctcggtgtcg gagatcacgc ggccgttgct gccggtcagc gtgaagccca
     8941 tgttcacggc cacctgattc accgccatca tcggattgcg cgtggcctgc gacgaaatga
     9001 ggccgcgcag cacgaagctc gcgccgagcc gccgcgacgc cgcgagtgcg gcatcggggt
     9061 cgttacggaa gtacgcgtcg atctcggcct gcgcgatctg gcggcgtatc tcgtccggcg
     9121 tgtacgtgcg aaggcccagt gcctgcagcc gcttgttgat ggcctgatag tgagggccgt
     9181 agttctgctg gtgcgcctcg acgaagccgt tcgactggcg ctcgccgatg atcaccatga
     9241 tcttgcggtt caggagcccg gcccggcagg gctgcgacag gtcctgcgcg atgcgctcgt
     9301 cgcgcgccgc cgcttcgcgt gcggcggtgt cgtcgggctg cgagaaacga aatccttgcc
     9361 cgacggcagc gccggcaacg agcgcaccca gcagcagtaa cgtcgaggaa gcctgggcac
     9421 gtcgcatggc ggcggaacga cgatgagggc aacgccgaag attagccgaa cgcctcgcgg
     9481 aaagccatgg caatggccgg tttgctgccg gacggcgggg cgccgcgcaa cggtgcgaaa
     9541 atgtgtcatg gacaggctgt ttcaccccgc cgtcgccgcg tggctggcga agacctttgc
     9601 ggcgcccacc gaggcgcagg cgcgcgcatg gcccgcgatc catgcgggcc gcgacgtgct
     9661 gatcgcggca cccaccggat cgggcaagac gctcgccgcc tttctggcgg cgatcgacgc
     9721 gctcgtgcgg cagggcgtgg agcatccgct gcccgacgag acgttcgtcg tctacgtctc
     9781 gccgctgaag gcgttgtcga acgatatccg caggaatctc gaggcgccgc tcgccggcat
     9841 ccggtccgag cttgcgtcgc tgggctcgcc cgacgtcgac atccgcacgc tggtgcgcac
     9901 cggcgacacg ccgcagcacg agcgcgcgag gatgcgcaag cgtccgccgc acatcgtcgt
     9961 cacgacaccc gaatcgcttt acgtgctgct gggttccgag tcggggcgag cgatgcttgc
    10021 gaccacccgt acggtcatcg tcgacgagat ccacgcgctt gcgtcgaaca agcgcggcag
    10081 ccatctcgca ctgtcgctgg cacgactgca ggcgctcgcc acgcatcggg tgacgcggat
    10141 cggactgtca gccacgcagc ggccgatcga ggaggtggcg cgcttcctgg tgggccgcaa
    10201 cgcggccgat gccgtcatcg tcgacacggg ccacgtccgt ccgcgcgatc tggcgctcga
    10261 agtgccgccg tcaccgctcg aggcggtgat gtcgaacgag gtatggctgc aggtgtacgc
    10321 gcggcttgcc gacctcgttg cgcggcaccg cacgacgctc gtcttcgtca acacgcgccg
    10381 catgtccgag cgcgtgacac gtcacctgtc cgagctgctc ggcgccgcgc acgtcatggc
    10441 gcaccacggc agcatggcga aggagctgcg ccttgcagcg gaagcgcggc tgaagtgcgg
    10501 cgagctgaag gcgctcgttg cgacggcgtc gctcgagctc ggcatcgaca tcggagacgt
    10561 cgacctcgtc tgccagatcg gctcgccgcg ctcgatctcg acgttcctcc agcgcgccgg
    10621 ccgcgcaagc cacaccgtgc acggcgtgcc gaaggcgcgc ctgtttccgc tgtcgcgcga
    10681 cgagctcgtc gaatgcacgg cgctgctcga cgcggttcgc cgcggcgagc tcgatcgcgt
    10741 cacgattccc gggaagccgc tcgacgtgct cgcgcagcag atcgtcgccg aggtcgcggc
    10801 gcacgactgg cccgagcgcg ggctttacga catgctgacg ggtgcgtatc cgtatgccac
    10861 gctcgaatac gacgactatc tcgacgtcgt gcgtacgctc tcggacgggt acacgacgcg
    10921 ccagggccag cgcggcgcgt ggctccatcg cgatgcggtc aacggcgtgc tgcgggcacg
    10981 ccgcggcgca cggatgaccg cgctcacatc gggcggcgcg attcccgata ccgccgatta
    11041 cgacgtgatt ctcgagcccg cgggccacat ggtgggtacc gtgaacgagg acttcgccgt
    11101 cgaaagcctc gccggcgacg tgttccagct cggcaacacg tcctatcgga tccggcgcat
    11161 cgaagcgggc cgcgtgcggg tcgaggatgc gcaggggcag gcgccgaaca ttccgttctg
    11221 gctcggcgaa gcccctgcgc ggagcgacga gctttcgttc gccgtgtcgc gcttgcgaag
    11281 cgaccttttc gcttccgcgc gttcgcacgc cgggccggcc gacgccgcgt ccgcgcttgc
    11341 cgcactcgac gcgtccggca tggagccggc agccgcgagc cagctcgtcg actatctcgc
    11401 gaacgcgcgc tcggtgctcg gtacgctgcc gacgcaggac aggatcgtgc tagagcgttt
    11461 ctttgacgag tcgggcggca tgcagctcgt gatccacgca ccgttcggca gccgcatcaa
    11521 ccgcgcatgg ggattggcgc tgcgcaagcg cttctgcgtc aggttcaact tcgagctgca
    11581 ggccgccgcc accgaggatg cgatcgtgct gtcgctgtcg acgtcgcaca gcttcgcgct
    11641 tgcggacgtc gcgcactacc tgcattcgaa cacggtgcgc gcggtgctgg tgcaggcgat
    11701 gcttgcggca ccgatgttca ccgcgcggtg gcgctggatt gcgaacacgt cgctcgcgct
    11761 gccgcgcttt cgcggcggga agaaggtgcc ggcgccgttg cagcggatgc gtgccgacga
    11821 cctgatggcg gcggtgtttc ccgaccagat cgcgtgtgcg gagaacatcg tcggcgaacg
    11881 tgaaatcccc gaccatccgc tggttcgcca ggtggttcat gattgcctgc acgaagcgat
    11941 ggacatcgac gggctcgagc ggcttctgcg tcgcatcgag cgcggtgacg tcgagatcgt
    12001 cgcccgcgac ctgcctgcgc cgtcgcccct ggcgatggag attctcaacg cgcgaccgta
    12061 cgcgtatctc gacgatgcac cgctcgagga gcggcgcacg caggcggtga tggcgcgccg
    12121 ctggaccgac gacgatgcgt cgggcaagct cggcgagctc gacgccgcgg caatcgagcg
    12181 cgtgcgcatc gaagcgtggc cggaggcgtc acgccccgac gagatgcatg cggcgctgct
    12241 cgcgctcgga ttcgtcaccg atgcggaaat tcgcgcgcac gacgggtggc gcgagtttct
    12301 ggaagcgctc gcgcacgacc ggcgcgcgac ggcggtcgcg cttgccgcgg agcaccgcgt
    12361 cttcgccgcc gcggagcgcc tgccgcagtt cgacgcgctc tatgcgggcg ctgcgcatgc
    12421 gccgccgatc ggcgcgccgg ccgaatacgc cgagcgcgca tggtcgcgcg acgaggcgct
    12481 caccgagctc gtgcgtagcc gcctgcaggc gctcggcccg gtcacggcga gcgcgatcgc
    12541 cgattcgttc gcgttgcccg tgggtgaaat cgaaggcgcg ctcgcacgcc tggcgggcga
    12601 aggcgtcgcg atgaacggaa gctatacgcc ggatgcgacg cgcccggaat ggtgcgaccg
    12661 cacgcttctc gcgcgcattc atcgctacac gatcaagcgg ctgcgccagg aaatcgagcc
    12721 cgtgtcgacg caggacttcg tgcgcttcct gtttcgctgg cagcacgtgg cccccgacga
    12781 catgcgacag ggtccggatg cgctcgacgc gatcatcggg cagttgcagg gtttcgaggc
    12841 cccggccgct gcatgggaat cggaaatcct ccctgcgcgc ttgtccgatt acgagttcac
    12901 ctggctcgac gacctgtgcc tgtcgggtcg cgcggtctgg gcgcgattga cgccgtcatc
    12961 cggcggcgcg acgaacgtga tccggaccac gccggtcacg ctgttgccaa ggcgcagcgc
    13021 ggccacgtgg atcgccgccg cgccctcggg cgacgaggac gccgttgcgg caagcgcgcg
    13081 cgcgcaagcg gtggcggcgt tcctgcgcgc aaacggcgcg tcgttctacg acgagatcgt
    13141 cgacggcgtg cacctgctgc gcacgcaagt cgaggacgcg ctcgccgagc tcgtggcgct
    13201 cggcagcgtc acctccgaca gctttgcggg ccttcgtgcg ctgttgacgc cgtcgtcgaa
    13261 acgcaagcgc cttggcggcg gcaagcgcca tcggcgcacg gcgctgttcg gcatcgagga
    13321 cgcggggcgc tggtcgctcg cgcgccgcgc gcctcgtccc gcgcaggaca cggcggcgca
    13381 cggatggacg cgcagcgatc ctgcacacgc gatcgtcgag cacgttgcgc atgcgctgct
    13441 gcgccgttac ggcgtcgtgt cgtggcgcat cctcgagcgc gaggcggcgt ggctcccgcc
    13501 gtggcgcgaa ttgtcgcgcg tcttgcgccg gctcgaggcg cgcggcgaca tccgcggcgg
    13561 ccgcttcgtc gcgagcatca ccggcgagca gttcgcgctg cccgaggcgc tggcgctgtt
    13621 gcgcgagacg cgccgtgcgc ctgcggcggc gcatttcgtg tcgatcagtg catccgatcc
    13681 gctgaacgtc gtcggcacgc tgctgccggg cgcgagaatc cccgccatta ccggcaaccg
    13741 cgtcctgtac cgcgacggtg ttgccgtggc cgccctcgtc gcgggcgaga tgcgcatgct
    13801 cgtcccgctc gagcaggccg acgcgcgcgc cgccgagcat gcgttgatcc gccacccgcc
    13861 cggcgcggca ccgttcgcgt acctgcacta agggtcgacg caacgacgct cgctggcctg
    13921 caccgcgccg gtgcacgaat attcgcgcaa cggcgcgcgc cgggaacgct tctttcggcg
    13981 atactgtcag agattgcttc gagcgcggac tcctcctcgc ggcgcctggt catcgtcacc
    14041 ggcgcagccc gtgtccgcgg cgacgggcaa cgacgcaatg gaacgccaga cagaactcga
    14101 ttggttgcgc ggcttgatgc tcgtgctgat gacgatgacg catctgccca cgtggttcag
    14161 cgcgcacgcg gggcagccct ttggattcgt gtcggccgcc gaaggcttcg tgttcctgtc
    14221 ggcgttcctg gtcggctcgg tctacgcgca caaggcgcgc acgcgcggct acgcggtgat
    14281 gcggcgcgcg ctatggggtc gcgcgctgaa ggtctacctc gcgcacatcg gcctcctgct
    14341 gttcctgctc ctcgtgctgg tgccgatcgc cgtctcgcac aatgcgcagg caatcaccgg
    14401 gcttgcatcg tattacctcg agcggccgca tctcgcgctc gtgagcgggc tgattctcgc
    14461 gtacaacccg ccgctgctcg acatcctgcc gatgtatgtg ctgttcctgg cactgtcgcc
    14521 actcgccctg cgccttggga cgcagcgcgg atgggcgctg ctgctggcgc tgagcgccgt
    14581 gctgtggctg ttcgcgcaat acgacggtgg cctgcacgtc tacaagtcgc tggcggcgct
    14641 cgtcggctgg cccgtccctt acggcgccac cggcgccttc tcgttcatgg cctggcaatt
    14701 gctgtggctc gtcggattgc ggctcggcgc ggtcaatggc gtggccgacc tgccgcgatc
    14761 gccgatcggc gatcgccgct ggccggggtt cggcatgctc gcggcgatga gcgtcgccgc
    14821 ggtgttcttc acctggcgcc acgtcgtggg gcaggcgccg ttcggctcgg acgcgatgct
    14881 caatgcgcta ttcgacaaat ggcacctcgg tccgctgcgg cttttgaact tcgccgtgct
    14941 cgcgctgatc gtcgtgcatg tgcgtcccgt cctcgtcgaa tgggccgcac actcgtcgat
    15001 cgcaacgctc gggcgcgctt cgctgacggt gttcagcgcg catctgctga tctgccttgc
    15061 actgctcacg atcatcggcg aagccgcgcg tccgcatctc agcgtgttcg aaacactgtt
    15121 gctcctcgga acgcttgccg cgctgtacgg cgttgcgcaa gcgacgctcg cggcgccgcg
    15181 ggtcgtacgc gccggcagtc gcgcgctcgc cgcgcggctc agtgcacgtg gggcgcgatg
    15241 acgcgcttcc acagtgcgta gccgtcggtg ttgagatgca gcgagtccgc ccggaacagc
    15301 tccgggcggg gtcggcctga cgcgtcgagc atcggcgtga acacgtcgat gtagtcgacc
    15361 ttgccctcgc gatcggcgta atcgcggatc agcgcgttcg tcgcgcggat ctgcggcagc
    15421 agcgcgacgc gggcggggct gggcttgatc gagatgtaga agatctcggt gtcgggcagc
    15481 gcctggtgca cgccgtccgc gaacgcggtg aaccggtgca gcacctcgct cggcgcgctt
    15541 ccggtggcaa ggtcgttgtc gccggcatag acaagcaccg tgcgcggacg gtagcgcagc
    15601 accagccggc tcaggtgctt gacgcagtcg gaaagctgcg agccgccgaa gccgcgcttg
    15661 atgaccaccg gcaggtcgtg gaactgcgat tcgaggtcgc tccacaggcg aatcgacgag
    15721 ctgccgacga acagcacgcc gccggccgga tgcggatggg cctcgtcatc cgcggcgaac
    15781 gcggcgaatg cggcgtccca ccgggatccg ggaatcggtg catcgggcag cgcggcggcg
    15841 ccggcggtgc cgatggatgc gaagaccgtc agtgcagccg cgaggagaag cttgccgttc
    15901 atttcagcgc atgcaggagt cgagtttgcc ttcgtcgggc cagcgggccg ggcgtgggcc
    15961 cctggcggtt atcgagcgcc ggttaaacgg ataccggagg taacgcgatg acacgacttt
    16021 caggcaggcg acggcggccg ggccgccgcc gaacgtacca cgtctctgcg ggcgaggctt
    16081 tcggccggcc agtagcgctt gctgtcgtag tactcgggca tcgccggcgt gccggccggc
    16141 aggatgaccc aaggctgttt ggacagcgtg aagatgtgaa tgtccggcgg gaggcgatcg
    16201 ggctcgtcca gcgtgcccac gcggacgaac ttgaacaggt cgccagcgcc agcgtagtgg
    16261 ctccacagcg cgacctggca gtcgggacag cggacgatct tctggccacg gccgctgttc
    16321 gatggcgtgg cgaccgtgac tggatcgccc tgcagcacga cgacccggtc ggattcgatc
    16381 agcgcgttca gcacgaaggc actgccggtt tcgcgctggc accagcggca atggcagcaa
    16441 tggacgaaca gcggcttcgt cgtcatccgg tagcgcacgt tgcggcacgt gcatccgccg
    16501 tcgatcggat aggcatcgtt catgaggccg gcggtccctt gagctcgacc atgttgcctt
    16561 ccgggtcggt cacgtagatc gacggacctt ccccttccgc gccgtaacgc ggtaccgctt
    16621 cgccggcagc gattcccgcc gcctggaggt gccggcggat agcgggttcg tcgaacggtt
    16681 cgacgcggag gcagaagtga tcgaggttgc ggccttcgga gcccggcgcc gcgcccccgg
    16741 cgcggccgag cttgccgtcg acgggaacga gatcgatcat cgagcgtccg gcacgcaatt
    16801 gcacgaggcc gagctcatcg cgcctgcgct cgatgctgca accgaggaca tcgcggtaga
    16861 acgcgagcat ggcctcgacg tcgacgaccc gcagcaccag gtggtcgata tcgcgaatgc
    16921 ggatcattgg ttgcgtttac ggcggtgagc gatcaggcgt tttcgtagat cgaaacttcg
    16981 atcaggttcg cgtcggggtc gcggaagtag accgatcgta tcggccccgt ggcgccggtc
    17041 ttggcgaccg gtccctcgat gatcggcacc gcgcacgcct cgagctgcgc gatcacctcg
    17101 tcgagcgtcc ccgtggcgat caggcaaagg tcgagcgagc cggaggaggg atgcgccgcc
    17161 ttcggctcga attcgcggcc ggcaagatgc aggttgatct tctggcggcc gaacgcgagc
    17221 gccgtgcggc cgccggcgaa cgtcacgacg cgcatgccga gcacgcgcga gtagaagtcg
    17281 caggttgcct cgatgtcgcc gaccgtcatc acgacgtggt cgatccgatc gatgttcagc
    17341 gcattcatgc gatgttttgc gcgtgccgac gcggggcggc cgtcgcatgc gccaaccgtc
    17401 gcgattcgcc ggtcaatgcg cgcatcgcgg cggcccttgc atgaagcagc gcggtatcga
    17461 aaagcggcac ggcaacatcg ccggcgtcga tcagcatggc gatctcggtg cagccgagca
    17521 cgatgcctct tgcgccgcgc tcgaccaggc gcccgatgat cgagcgatag cgcacccgcg
    17581 acgcgtcgcg caccacgccc cggcaaagct cgtcgtagat cacgcgatgg atgcattcgc
    17641 gctcgtcgtc gtcgggcacg atcgccgtga tgccgtgcgc ggcgagccga tcccgataga
    17701 agtcctgctc catcgtgaac cgcgtgccga gcaggccgat gctcgacagg cccgcgtcca
    17761 cgattgcggc cgccgtcgga tcggcgatgt gcagcagcgg gatggtgacg gcccgcgtga
    17821 tcgccggtgc aaccttgtgc atcgtgttcg tgcagagcac caggaagtcg gcgccgccgg
    17881 cctcgaccga acgtgcggcg tcggcgagga tctcgccggc ccgcgcccag tctcccgcgt
    17941 cctgcagccg ctcgatctca tgaaagtcga cgctgttgag gacgatgctg gccgaatgca
    18001 gtccgccgag cgcgtcacgg acggcttcgt tgatcgtccg gtagtacgga agcgtcgatt
    18061 cccagctcat gccaccaagc aggccgatcg tttgcatgat catcccggat gaactttcgg
    18121 gaattgtcgc atgggccggt attcttcgtt caccgcctgc agcgcatcga gcgtctgcaa
    18181 tgcgctctcg acgtgcgcgg gcaccacgcg acgctcgcgc aacacccatg ccagcccgcg
    18241 cagcgcatca cgcgcgttgc gcagggcgac gccatcgcgc cggcttgcgg cccaccagtc
    18301 acccgtcgcc cgccacgctg tgcgcattgg ccggcgaagc caggcgatcc acagcgcgtt
    18361 tcgcgcgctc atccgcgacc ggcgtgccgt gtcgcgcacg cacgacggaa aatggcacac
    18421 gaccgcgtcg ggaacgtacg ccatgtgcca gccggccgtc atcaggtcga tcgcgagcag
    18481 cgcttcctcg cgacccagaa acagtcgcgg atgatagccg ccggcgtcga ggaaggcggc
    18541 gcggcgcacc atgcatgcac cggcgagcat ccccagcaat tcgctgccgc gcacgccgag
    18601 cctgttcgga aacgggctcg cggccatcac ggcattggtc gggtcgtcgc gccgccgctc
    18661 gccgacgagc acgcgcgcgg tgactgctgc aagcgccgga tgcgcatcga gcgccgccgc
    18721 ggcgagcgcg atcgattcgg ggagccacca cgtatcgtcg tcgcagaagg cgacgtaggg
    18781 cgtcgtgcac acgcgcacac ccgcgttgcg gccggccgcg cccgcgttgc ttggcaggcg
    18841 aacgatgcgc acgcgcgggc aaaggctggc gagcgcctcc gccgtgccgt cggtcgacgc
    18901 attgtcgacg acgacgatcg gcacgctggc gtcgatttcg gcgagccgcg acagcgtgcg
    18961 cagcacctcg ttgcgacgat tgaacgtcag aacgacgagg ctgaccgtat cgccgatcat
    19021 cgcgaccctg cgctgcgcga cagtgcagag tgccgttcgg gcgcgaatac gtcgccgtgc
    19081 gcgttgcgtg tgcggagcgc gcgaagctgc gaccggtagc aaccgacggc gtgtcgcttg
    19141 cgtgccgcgg catcgcgcgc catcgcgggc gaccggcgct ggagaacgta accacgctcg
    19201 gccagcacac gcgttcgctc gtccaccgca cccgggatgc agcggtagat cgcgtcttcg
    19261 taaacatgcc acgcacgcgt ggcgaagcga tcgaacagcg cgagcgatgc gtcgctcgag
    19321 cggcgatgat ccgcgtggaa caatccgagc gggacgtgga ccgcagacgg cgcttcgtgc
    19381 gcgacgagct cggccaatgc gtcggtgatg actcgcgtgg gtcgtggctc gccgtactgg
    19441 tcgtcacgga agtcgagctg gatggcggcg gcgcccaacg aatcgagtgc cgtgcgatcc
    19501 tcgtggcgcc gaagcgcgat cacatcgtcg ccttcgccga acccgcattc gcgatcccat
    19561 ggtgtcaagg ttgcgcccgc gggagggaac ccggcgaaga tcgtcacgac cagcggactc
    19621 gcgcacgaag cgatccattc tccgcacgcg aacaccgcat cgtcgaggtg cggtgaaatg
    19681 aacaagcgcc gcgacgcatc gcgcatggcc ggcgtcagcg ccgcttccgc gtgaccgcgc
    19741 gtgccgttgc cggcgcagca gcgacggcga gcggcaggag ttccgcctcg atgcgatcgg
    19801 ccgcgatcgt gagccttccg accgaaaccg gcagccggaa ccggcgtggt ccggcactgc
    19861 cgggattgac gtacagcacg ccgtcgcggc gctcgatcgt cggctggtgc gagtggcccg
    19921 acacgacggc gtcgatgcct gcggcgaccg gctcggccgt gagctcggcg agatcgtgga
    19981 tgacgaggat gcacttgccg ccgacatcga ggcgctgcgt ctgcggcaaa ccggaccccg
    20041 gggcgccaac gtcgttgttg ccgcgaactg cgcacaccgg cgcaatcgcc gcaagccgct
    20101 cgaggagcga cccgtcgccg atgtcgccgg catgcacgat cacatcgctg ccgcgcaatg
    20161 ccgccagtgc ttctggacgc agcaacccgt gcgtgtcgga gatgagcccg atgcagacgg
    20221 cggattcgcg cccaacatgc atcgtggtca cggcattcgt gcggctcgag gtgcaagcat
    20281 gttcgcatcg cggacgatcg cccacgcgcc gtcgcgcttg cgcaggatcg acagtgcggg
    20341 tccggcaagg tgggtcgcgg cgccgccacc gatcggcgtg accttcaccg aaagctcgct
    20401 ccagcagtac gcgagatcgc cggaaacgac gatctcgcgc acctcgccgc gtgaatcgat
    20461 gcgcgagtgc gtcagcagcg ttcgcagccc ctgctcgaag gccctgcgac cgcgcatcgg
    20521 cgcgcgccct gcgccgagaa acacgacgtc ttccgccatc agcggcagga tgcgcgcgac
    20581 atcgccggcg gccgtcgcct cgtgccaggt gcgcaacagc gtgtggatgg ccgagtcgtc
    20641 gtcttccatg gatcagcgct cccgtagcga tcgcagcaat gttaggtaaa tcgtcgcgtt
    20701 gacggcgacg acgagaaagg caagcaggta ttggccgggg cgggtgaggc ctgccgggta
    20761 aatcacgcca agcaggtagt gttcgacgaa gctgccgcca tagcccgcca ggccggccgc
    20821 atcgcgaagg cgattttcgg cgaacgtcag cgggcagatc ttgccggtcg cttcaacgaa
    20881 cacagcccag gcaacggccg cgatgtgcgc cggcatcagc gcgtgccagc gtacgaccag
    20941 cagtccccca agcaccacaa acagaatgaa gcaaagatgc agcagcagta cggaatcggc
    21001 ggcgaatcgg gcgagcatcg ctggcgcagc cgggacattc atgccgccgg ctgccacgtc
    21061 ggccgctgaa aatgcccatg ttgccgtgct gcccagccgg cctcgaggaa catcgccacg
    21121 tgcggtttgc aacggagttg gcttgcttcg gtagcggtga aatagcgcac ctcgagcgtc
    21181 tcttcgccgt cggggcgcaa ctcaccgcgc accggcctcc cggcgaacac gctcgatacc
    21241 caggcgagcc ggtctccatt cgcgtaggtg ctggtgcaca cgcgtccacc gaaaacgccg
    21301 atcaggcgcg tgagctcgac gtagagtccg gtttcctccc acatttcgcg caccgctgca
    21361 tccgccggga gctcgtaggg ctcgatcatg ccgcccggcg tcgtccagtc gttgccttcg
    21421 gcgtggcgca cgagcagcac gcggttggcg tcgtcgaacg cgagcacgct caccgtcggc
    21481 acttccagca ccgtggtgcc gatcatcgcg cgcaggccgc gaatatagtc cgacatcggc
    21541 atgcgttgcc tcgctggttg cgcgtcgcgc gttcgtcagc cgagcgccgc cgccgcggca
    21601 tcatgcgccg caccgctgat caggccgctc attcgcgcaa acagtcgccg cagctcggcg
    21661 cggtgccgga cgcgaaagct cgcgctcgct tgggcaagct cgagctcgtt ggcaacggtc
    21721 atttcgcaga atgcgcgtag ctccgcctcg ccgatttcgt attcgccatt cgtgaaccgg
    21781 tcgacgaacg tcgactgcgc cggggtgccg atcctcggat gaaagcgctc gcgatcgcag
    21841 gcaccgtaca gataggccag cgcttcggcc tcgttcccta tgacctcggc gatggcggtt
    21901 cgtgcgtcga gccccgcgag ccggccggca atgccgccgg tgccgtagac ggcgtgatag
    21961 aggcccgcca tgcagaccgg ctcgcgattg ccccagcgac gcagcaatcg catcgtcgca
    22021 tgcaggtgtt gctcgagcgt gccgttgacg tgcgcaaacc gggcggcgcc aagtcgttgc
    22081 aattgcatgg cgaggcgctc gtcgcgacct gggaggtgcg cgctgcgccg gtcgatctcg
    22141 ccgtgctcgg catgcggcgc catggcaccg gtctgggcgg ggttcggcat atcgttcaag
    22201 tgtcgcgagt cgtcagggag tggttcccag cagtgcgccg accccggcgg tcaccgccat
    22261 tgccagcgca ccccagaatg tcacccgcgc cgcgccaatg ccgatgcggg caccgccggc
    22321 gcgcgccgca acgccgccga gaatcgccag aaacacgagc gacgtcgcgc acaccacggg
    22381 aatgagatgc gcctcgggtg cgactgccgt gacgacgaga ggcagcgcgg ccccaacggc
    22441 gaaacttgcc gccgatgcga gcgcggcctg gatgggccgc ggcctgatcg tgtcggaaat
    22501 gcccagctcg tcgcgtgcgt gcgcggcaag cgcgtcgtgc gccatcagct ggtcggccac
    22561 ttgccgtgcg agcgcctgat cgagtccgcg ccgaacgtag atggcggtca attcgttgcg
    22621 ctcgccttgc aggtcggacg tgagctcctt ccgctcgcgc tcgagatcgg cgtgctcggt
    22681 atccgcctgc gaatgcaccg agacgtactc cccggcggcc atcgacatgg cacccgcaac
    22741 gaggcccgcc accgccgcga cgacgatgtc ggcgtgcgtc gcatgcgcgg ccgcgacgcc
    22801 ggccacgagg ctcgcggttg acacgatgcc gtcgttcgcg cccagcacgg cggcgcgaag
    22861 ccagccgatg cgattggttc ggtggcgttc acgatggcgc cgcgcgctcc acatcacggg
    22921 gtatgcacgg cgtgcatttt gatggcgatc gttcggctat cgctcatgcg gcggaatcga
    22981 cttgatcgcc gctgcgaacc gcgcatgcgc ttgcggatcg acgggcctgt gccgaacccg
    23041 gatgcctgcg gcatccgtgc cgaggaagtt gtcgcccttc cagttgcgcg ccggcctgat
    23101 cggcctcggc gtgaatccac caccgcagtt cgggcaaacg ttctgcagca cggcttcgac
    23161 gcacgcggcg caaaacgtgc actcgtagct gcagatccgt gcgtcgagcg cgtcggccgg
    23221 caaagccttg ttgcagttct cgcaggtggg tctcagctcg agcatcgcgt gatcttgccg
    23281 attcgggatt cccgcattgt agtggtcggc ggcgattcgc ctcattgggc atgctcaacg
    23341 cgcgcgactg cggtgtctac gaggcaacat tgcggatcac ccgacaggga ttgccggcgg
    23401 cgagcgcgcc gtccggaacg tcgcgggtga cgacgctgcc ggcgccgatc acggcccctt
    23461 cgccgatatt cacgccggca aggatcagcg cgccaccgcc gacccacacg tccgcgccga
    23521 tggtcaccgg ctttccgaat tcctgttgcc tgcgcgcccg tgcgtcgagc ggatgcagcg
    23581 gcgtgagaat ctgcacgccg ggaccgaaca gcgtgtagtc gccgatgcgc acatcgcaga
    23641 cgtcgagcac cacgcaattg aagttgaaaa agacgcgtgt gccgaggtag atgttcgagc
    23701 catagtcgca gtggaagggc ggctgcatcc ctacgctgtc gccgcccgca gcgaacagcg
    23761 cgcgcaggag ctggcgtcgt tgttgctcgg gcgattcgcg cgtgaggttc agtgcgcggc
    23821 acagctcgcg cgcacgctcg cgcgccgccg tgagctcggg gtcgagcggg tcgtacggtt
    23881 gtcccgcgag catcagttcg cgctgggtcg tcattcctgc cttttggcga cgacggttgg
    23941 gcgtcggggc cgtcgacgac cagtaccggc gcggccttcg gcccttcagc cggatttgcg
    24001 caggaagaca tgcgcgactg cgcgcgtccc cgcaacgtgc ccg
//
LOCUS       DASSVX010000275         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1262057, whole genome shotgun sequence.
ACCESSION   DASSVX010000275 DASSVX010000000
VERSION     DASSVX010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262057"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFO53_11840"
     CDS             complement(<1..570)
                     /locus_tag="VFO53_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877013.1"
                     /translation="MLRFLFFVAALAASAVAGAQTVTFGASVQLTGPVANTGRYYKDA
                     YEFAIEKINAAGGVKVGSAQQKIALKLLDNQSDVNLSVRQYSQLLSQDKVNFLLGPFA
                     SNFVLADSSIAEKSSVPMVQGGGASDEIYTRGYKYIFGTLPAASDYFGSTIDALAKLN
                     PAPKSVALLYADDSFDVSVAKGTRDLLKKA"
     gene            complement(614..2050)
                     /locus_tag="VFO53_11845"
     CDS             complement(614..2050)
                     /locus_tag="VFO53_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8877014.1"
                     /translation="MEAAGNYIAGEWLEATQAAEDINPSNTADVVGLFARADANAAAR
                     AIAAAKDAFPAWSRSGIQQRHDILKRIGDDILAHKDEIGRLLSREEGKTIAEAVGETV
                     RAGQIFLFFAGECLRQAGEKLASVRPGIDIEITREPLGVVGLITPWNFPIAIPAWKIA
                     PALAYGNCVVFKPADLVPGTAHALAKIIAAAGIPAGAFNLVMGRGSVVGEALLTHPDV
                     AAISFTGSVATGRAIAAKCVAGMKKFQLEMGGKNPLVVLDDADLGVAVECAANGAFFS
                     TGQRCTASSRLIVTTGIHDRFVQALTERMKSLVIDDAQKPGTHIGPAVDESQLAQDLR
                     YIDIGRQEGARLVAGGERLGRDAPGFYVAPALFTEVSNDMRIAREEIFGPVAAVIRAR
                     DYDEALALANDTAFGLCAGVCTTSLKHATHFRRNADAGMVMVNLPTAGVDYHVPFGGR
                     KGSSYGPREQGRYAAEFYTTVKTAYTLA"
     gene            complement(2092..2802)
                     /locus_tag="VFO53_11850"
     CDS             complement(2092..2802)
                     /locus_tag="VFO53_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HET8877015.1"
                     /translation="MRDATRAKLASVSTATLTTALFKRGLRNQFVQDVHPLNANPAPM
                     VGEAYTLRYIPAREDLNPITVFRDRAHPQRRAVEECPAGAVLVIDSRKDARAASAGSI
                     LVTRLMKRGVAGVVTDGGFRDSPDIARLSIPAYHQRPAAPTNLTRHQAVDINVPIGCG
                     DAPVFPGDVVVGDAEGVVIVPAHLADEVADEAVEMTVFEDFVTEAVQNGRSILGLYPA
                     TDERTVRDFAAWRAAKGR"
     gene            complement(2831..>3589)
                     /locus_tag="VFO53_11855"
     CDS             complement(2831..>3589)
                     /locus_tag="VFO53_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877016.1"
                     /translation="NVDVVNTGVGNLLLLWDRTKGGVKGIVANSALPLALITRNPNIH
                     TLKDYGPNDRIAVPTLKVSTQAILLQMASEKTFGADQTTKLDANTVQLGHPDAAIMMA
                     NPQGEITSHFAAPPYSYYELRNVKGAHVVLSSPDVIGGPLTQSQFFTTTKFADANPKI
                     VAAIKAATFDAIDFIRKDTPAAVEIYREVTHDKMPAAEIVDMLKQPGMMEWGAAPQGT
                     MKFAAHLFKVGTLKTMPKAWTDYYLPIIADLPGN"
BASE COUNT          557 a         1175 c         1215 g          642 t
ORIGIN      
        1 ggccttcttc agcagatcac gcgtgccctt ggccaccgac acatcgaacg aatcgtcggc
       61 ataaagcagc gcaacgctct tcggcgccgg attgagcttc gccagcgcgt cgatcgtgct
      121 gccgaagtaa tcgctcgccg ccggaagcgt gccgaagatg tatttgtagc cgcgcgtata
      181 gatttcgtcg gaggcgccgc cgccctgcac catcggcacc gagctcttct cggcgatcga
      241 cgaatcggcg agcacgaagt tgctcgcgaa cgggccgagc aggaagttca ccttgtcctg
      301 cgacaggagc tgcgagtact ggcgcacgct caggttgacg tcggactggt tgtcgagaag
      361 cttcagcgcg atcttctgct gcgccgaacc caccttcacg ccacccgccg cgttgatctt
      421 ctcgatcgcg aattcgtacg cgtccttgta atagcgtccg gtgttcgcga cgggcccggt
      481 cagttgcacc gaggcgccga acgtgaccgt ctgcgcgccc gccacggcgg aagcggcgag
      541 ggccgcaacg aagaacagga aacgaagcat catgccgtcc accttttgtt gttgatggga
      601 tgcggcgctg cgttcacgcg agcgtgtacg ccgtcttcac cgtcgtatag aactcggcgg
      661 cgtagcggcc ctgctcgcgc ggcccgtagc tcgatccctt gcggccgcca aagggaacgt
      721 gatagtcgac cccggcggtc ggcaggttga ccatcaccat gcctgcatcg gcattgcgcc
      781 tgaaatgcgt cgcatgcttg aggctcgtcg tgcagacgcc ggcgcaaagg ccgaacgccg
      841 tgtcgttggc gagcgcgagc gcttcgtcgt aatcgcgcgc acggatcacc gccgcgaccg
      901 ggccgaagat ttcctcgcgc gcaatgcgca tgtcgttcga gacctcggtg aaaagcgccg
      961 gcgcgacata gaagcccggc gcgtcgcggc cgagccgctc gccgccggcc acgagccgcg
     1021 cgccttcctg ccggccgatg tcgatgtagc gcaggtcctg cgcgagttgc gactcgtcga
     1081 ccgccggtcc gatgtgggtt cccggctttt gcgcgtcgtc gatcacgagc gacttcatcc
     1141 gctcggtcag cgcctggacg aaacggtcgt ggatgcccgt cgtgacgatc agccgcgacg
     1201 acgccgtgca gcgctggccg gtcgagaaga acgcgccgtt cgccgcacac tcgacggcga
     1261 cgccgagatc ggcgtcgtcg agcaccacga gcggattctt gccgcccatc tcgagctgga
     1321 atttcttcat gcccgcgacg cacttggcgg cgatcgcgcg cccggttgca acggagccgg
     1381 tgaacgagat ggccgcgacg tcggggtggg tcagcagcgc ttcgccgacc accgagccgc
     1441 ggcccatgac gaggttgaag gcgccggctg gaatgccggc cgccgcgatg atcttcgcca
     1501 gcgcgtgcgc cgtgccgggg acgagatcgg ccggcttgaa caccacgcag ttgccgtagg
     1561 cgagcgcggg tgcgatcttc cacgcgggaa tggcgatcgg aaaattccac ggcgtgatga
     1621 gaccgacgac gccgagcggc tcgcgggtta tctcgatgtc gatgcccggg cgcaccgacg
     1681 cgagcttctc gccggcctga cgcaggcact caccggcgaa gaacaggaag atctggccgg
     1741 cgcgcaccgt ttcgccgacc gcttcggcga tcgtcttgcc ctcctcgcgc gacaggagcc
     1801 ggccaatctc gtccttgtgc gcgaggatgt cgtcgccgat gcgcttcagg atgtcgtggc
     1861 gctgctggat gccgcttcgc gaccaggcgg gaaatgcgtc cttcgcggcc gcgatcgccc
     1921 gtgcggcggc gttcgcgtcg gcacgcgcga agagacccac gacgtcggcg gtgttggacg
     1981 ggttgatgtc ctcggccgcc tgcgtcgcct cgagccattc gcccgcgatg tagttgcctg
     2041 ctgcttccat gtgcgctccc gttcgactgt ccgaaactgc aatcgtgcgc ctcagcgacc
     2101 cttcgccgcg cgccaggcgg cgaaatcgcg cacggtccgc tcgtcggtgg ccgggtagag
     2161 gccgaggatc gagcggccgt tctgcacagc ctcggtgacg aagtcctcga acacggtcat
     2221 ttccaccgcc tcgtcggcaa cctcgtcggc aagatgtgcg ggaacgatga ccacgccttc
     2281 ggcgtcgccg acgacgacgt cgcccggaaa caccggcgca tcgccgcagc cgatcggcac
     2341 gttgatgtcg accgcctgat gccgggtgag gttcgtgggc gccgcgggcc gctggtgata
     2401 ggcggggatc gacagccgtg cgatgtcggg ggaatcgcga aagccgccgt cggtcaccac
     2461 ccccgcgacg ccgcgcttca tgagccgcgt gacgaggatc gatcctgccg aagcggcgcg
     2521 tgcgtccttg cggctgtcga tgacgagcac cgcgccggcc ggacactcct cgaccgcgcg
     2581 ccgctgtgga tgcgcgcggt cgcggaacac ggtgatggga ttcaggtcct cgcgcgccgg
     2641 gatgtagcgc agcgtgtacg cttcgcccac catcggtgcc gggttggcat tgagcggatg
     2701 cacgtcctgc acgaactggt tgcgcaggcc gcgcttgaag agcgcggtgg tgagcgtggc
     2761 ggtgctcacc gaggcgagct tcgcccgcgt ggcgtcgcgc agcggagcgg gcgtgggcgt
     2821 agggttcacc tcagtttccc ggcaggtcgg cgatgatcgg caggtagtag tcggtccacg
     2881 ccttgggcat cgtcttcagc gtaccgacct tgaagaggtg cgcggcgaat ttcatcgtgc
     2941 cctgcggcgc cgccccccac tccatcattc ccggctgctt cagcatgtcg acgatctcgg
     3001 cggcaggcat cttgtcgtgc gtgacctcgc ggtagatctc gacggcagcc ggcgtgtcct
     3061 tgcggatgaa atcgatcgcg tcgaacgtcg cggccttgat cgccgcgacg atcttcgggt
     3121 ttgcatcggc gaacttcgtc gtcgtgaaga actgcgattg cgtgagcggt ccgccgatga
     3181 cgtcgggcga ggacagcacc acgtgcgcac ccttgacgtt ccggagctcg taatacgaat
     3241 agggtggcgc cgcgaagtgg ctggtgatct cgccttgcgg attcgccatc atgatcgctg
     3301 cgtcggggtg tccgagctgc acggtgttcg catcgagctt cgtcgtctgg tcggcgccga
     3361 aggtcttctc gctcgccatt tgcagcagga tggcttgcgt cgacaccttg agcgtcggga
     3421 ccgcgatccg gtcgttcggg ccgtaatcct tgagcgtgtg gatgttcgga ttgcgcgtga
     3481 tcagcgcgag cggcagcgcc gagttggcaa cgatgccctt gacgccgccc ttcgtccgat
     3541 cccacaacag cagcaggttg ccgaccccgg tgttgaccac gtcgacatt
//
LOCUS       DASSVX010000276        18059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_961959, whole genome shotgun sequence.
ACCESSION   DASSVX010000276 DASSVX010000000
VERSION     DASSVX010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 18059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18059
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961959"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..707)
                     /locus_tag="VFO53_11860"
     CDS             complement(279..707)
                     /locus_tag="VFO53_11860"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET8877017.1"
                     /translation="MTLRLDCTIDALNRRGDGLLPGLVGLRVIALREGHLDAELDVQP
                     RLCAPNGYLHAATIVALADTACGYGSLAHLPGGAVNFTTIELKANFLGTAKDGTIACS
                     ARAVHLGATTQVWDATVTRVRDARTIALFRCTQMLLYERR"
     gene            complement(725..1777)
                     /locus_tag="VFO53_11865"
     CDS             complement(725..1777)
                     /locus_tag="VFO53_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HET8877018.1"
                     /translation="MSIEALSPQFGSDNYAGICPEAWRALEAANRGHAPGYGGDDWTC
                     AAIDAIQRTFETDCDVFFVFNGTAANSLALAALCRNTDAIVCHALAHVNVDECGAPEF
                     MSGGARLLTVDAPLAKLTPDAVIAKSVTPHDEHSSRPRAVSLTQATELGTVYTRGELA
                     ALCDTAHGRGMRVQMDGARFANAVASLDCSPADASWRAGVDVLCLGGTKNGLPAGEAI
                     LFFDRALADEFARRRKQAGQLASKMRFLAAPWLGVLENGAWLAHAAHANAMAKRLAHG
                     LSTLREARLLAPVEANGVFVDLPKHAIDALHGKGWRFYVFVGDTGVRLMCAWDTPAEV
                     VDRFVDDVRAVLAHAR"
     gene            complement(1774..4401)
                     /gene="alaS"
                     /locus_tag="VFO53_11870"
     CDS             complement(1774..4401)
                     /gene="alaS"
                     /locus_tag="VFO53_11870"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET8877019.1"
                     /translation="MKVADIRRQFIDYFVSKGHTHVPSSSLVPANDSTLLFTNSGMVQ
                     FKDVFLGTDTRPYVRATSVQACLRAGGKHNDLENVGYTARHHTFFEMLGNWSFGDYFK
                     RDAIRYAWQLLTEHYRLPAERLWVTVYIDDDEAYDLWTRDIGVPAQRCIRIGDNKGAR
                     YASDNFWQMADTGPCGPCSEIFYDHGPGVFGGPPGSADAEGDRYIEIWNLVFMQFDRQ
                     KRGDEFTMTPLPRPCVDTGMGLERLAAVLQHVHSNYEIDLFTTLIEAAARETRTSDRD
                     SPSLKVIADHIRACAFLIVDGVMPGSEGRGYVLRRIIRRAIRHGYQLGQKRPFFFRLV
                     DDLEREMGEAYPGLARDKVRVAQVLKAEEERFGETLENGMRMLGEALRDGKRVLDGET
                     AFTLYDTYGFPLDLTADVAREAGITVDAAGFDRAMDAQRERARAASQFKSGEALAYGG
                     PKTAFRGYESLSEEGRVVALYKEGARVESLSTGERGIVVLDRTPFYAESGGQVGDRGE
                     LSKGGACLTLFAVDDAQKVQPDVVGHGGEMKTGEWRAGDRVAATVDCEARGRTMRNHS
                     ATHLMHKALREVLGGHVQQKGSLVDPGKTRFDFAHDAPLTDAEIRRVEAIVNAEILAN
                     EGVSARTMPIAEAQKSGAMMLFGEKYGDEVRVLDIGSSRELCGGTHVARTGDIGLFKI
                     VSESGVAAGIRRVEAVTGKGALDYVQDQEAKLAGVARLLKAPLAELEARIAQLQESAR
                     NTERELARLKAKAAASAGDDLAGNAIDVKGVKVVAAALEASDAKALRETIDKLKDKLE
                     SAAIVLASVADGRVTLIAGVTSDLTGKVKAGDLVNHVAQQVGGKGGGRADMAQAGGSN
                     PAALPKALQSVSGWVGDRL"
     gene            complement(4525..4977)
                     /locus_tag="VFO53_11875"
     CDS             complement(4525..4977)
                     /locus_tag="VFO53_11875"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HET8877020.1"
                     /translation="MLARRDYPRAALEQRLAGRGADPAEVTRTLDELERLGYLSDARF
                     AHMLVAQKSARFGKRAIAHAMRAQRVEATAARAALDTLAGSDELALARAVWARRFRSA
                     PGDERERARQIRFLIARGYGVAVALAVVGRSCADACTDHEAGTDIGDG"
     gene            complement(5064..6128)
                     /gene="recA"
                     /locus_tag="VFO53_11880"
     CDS             complement(5064..6128)
                     /gene="recA"
                     /locus_tag="VFO53_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET8877021.1"
                     /translation="MISGQTSDDNKVKALAAALAQIEKQFGKGSIMKMGEAQIQNDLT
                     VVSTGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLCLQVVAQVQKAGGTAAYIDAE
                     NALDPVYAGKLGVNVGELLISQPDTGEQGLEIADMLVRSGGVDIIVIDSVAALVPKAE
                     IEGEMGDQLPGLQARLMSQALRKLTSNIKRANVLVIFINQIRMKIGVMFGNPETTTGG
                     NALKFYASVRLDIRRIGSIKRGDEVVGNETRVKVVKNKVSPPFREALFDILYGEGISR
                     EGEIIELGVNQRIIEKSGAWYAYAGDKIGQGKDNAREFLREHPDMAKELEAKIREAVG
                     VPSPPPIKGNGASHGNGADV"
     gene            complement(6242..6754)
                     /locus_tag="VFO53_11885"
     CDS             complement(6242..6754)
                     /locus_tag="VFO53_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HET8877022.1"
                     /translation="MSIEETQERLAMALGHALLARGMRVATAESCTGGLVAAAMTAIA
                     GSSEWFERGFVTYSNDAKCELLGVPRDVLETAGAVSEAAARAMADGALARSHADCTIS
                     VTGIAGPGGGSAAKPVGMVCFGWTLPTPPTEVMTRHFDGTRGGIRAAAVEAALAGLIE
                     LLDTRLPLSR"
     gene            complement(6903..7403)
                     /locus_tag="VFO53_11890"
     CDS             complement(6903..7403)
                     /locus_tag="VFO53_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HET8877023.1"
                     /translation="MQTATMKAPTFAFLVRHPAHFIALGLGTGLSPVAPGTFGTLVAL
                     PLAWLLDAHAATWGWLVAIAAITIAGAWCAHITARDLGVPDHGSIVIDEIAAFLLVLF
                     FAGAAPWRQALAFVLFRFFDIVKPPPIREIDRAMKNAAGVMADDFAAAGYTLLVLAVG
                     ERLLGS"
     gene            complement(7408..8376)
                     /gene="thiL"
                     /locus_tag="VFO53_11895"
     CDS             complement(7408..8376)
                     /gene="thiL"
                     /locus_tag="VFO53_11895"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET8877024.1"
                     /translation="MNEFEVIERYFRRPPRNGDVRIGIGDDGAVVAPAAGMEYVLTVD
                     MLVEGRHFAAGADPRQLGHKTLAVNLSDLAAMGALPRFVLLAGALPVADPDWLQAFMR
                     GFDALAAQYDVELIGGDTTRGPRTFCVTAIGELPAGTAITRGGANPGDTIFVSGRLGD
                     AALALAVAGRRVEIDPTLLPALQRRLDAPEPRIALGVALRGIASAALDVSDGLTGDLR
                     HILDASRVGATIDLAAIPCSDALRALARGPQRLLALACLLAGGDDYELCFTATPMMRE
                     RVDALAHALSVPLTPIGAITAEPGLAIRDEHGTPMASLPHAFDHFA"
     gene            complement(8373..8858)
                     /gene="nusB"
                     /locus_tag="VFO53_11900"
     CDS             complement(8373..8858)
                     /gene="nusB"
                     /locus_tag="VFO53_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET8877025.1"
                     /translation="MAGSGSEHAGRHRARELVVHGIYERQMSRRPDRDIAASLIAEPG
                     HAAAEEADAADTAGFDAAYFDELWRGVTGEWDALLAQLAPHVDRGIDALAPIERAVIT
                     IGAWELAHRLDIPYRVVINEAVELAKRYGGTDGYKFVNGVLDKVAARERADEIRVSGR
                     R"
     gene            complement(8848..9312)
                     /gene="ribH"
                     /locus_tag="VFO53_11905"
     CDS             complement(8848..9312)
                     /gene="ribH"
                     /locus_tag="VFO53_11905"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET8877026.1"
                     /translation="MPILRIEPNLRGDERRVAIVQSRFNPRIGEGLLAGALRALHEAG
                     VAEERITLITVPGALEIPLALQRLAQSGDHDVLVAIGAVIRGDTYHFEIVANESAAGV
                     ASVQLEFGVPTGNGILTCDTDAQAEARMAAKGSEAALAALELANLLDAIDGR"
     gene            complement(9368..10471)
                     /gene="ribBA"
                     /locus_tag="VFO53_11910"
     CDS             complement(9368..10471)
                     /gene="ribBA"
                     /locus_tag="VFO53_11910"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET8877027.1"
                     /translation="MPIAPIVEIVAEIRAGRMVVLVDEEDRENEGDLVLAADHVTPEA
                     VNFMVRFGRGLVCMAITERRARQLDLAPMTRVNRSVHGTNFTVSIEAAHGVATGISAA
                     DRAHTIRVAAAAHATPDDIVQPGHVFPLIAQPGGVLVRAGHTEACCDLAQLAGLSSSA
                     VLCEILNDDGTMARLPDLEVFASAHGLKIGAIADLIQHRSRTERLIERVAERPLRTPA
                     GTFRLVVYRDKLTDATHLALTRGPISPDVETLVRVHEPLSVVDLLDSESDSHAWSILE
                     AQHAIAAAGRGMIVLLHRPESAQELRQRAVAGDVPASSKMDLRNYGIGAQILRDLNVG
                     RMRLLARQRKMPSMTGFDLEVTGYEEAPARRAS"
     gene            complement(10564..11403)
                     /locus_tag="VFO53_11915"
     CDS             complement(10564..11403)
                     /locus_tag="VFO53_11915"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8877028.1"
                     /translation="MTPDEIERGYNNRAAVPDHQRWLDEWIARSATAQQALSPAIDLA
                     YGSGADETLDLYVPTKAARGTLMYIHGGWWRSLDKSDYGFVAPAFVDAGFAVALVNYT
                     LCPRDTIANAVEQCRRALLWLVREGPARGAPGPIVIGGHSAGGHLTAMMYAIDWRAHG
                     LADAPFVGGASLSGVHELAPLVQFSHNVDFRLDDAEARRMSPAMATPTTDVPLLLAVG
                     ADETSEFVRQTDLLWEAWPRNRARGVAQPMHVAGRNHYSVVLDYADPASRLTKATVAL
                     FGS"
     gene            complement(11425..12135)
                     /locus_tag="VFO53_11920"
     CDS             complement(11425..12135)
                     /locus_tag="VFO53_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET8877029.1"
                     /translation="MQYAIPAWDLPAVPVAGGQRQFPVRHVFCVGRNYAEHAKEMGGD
                     ATKEPPFFFTKAADAIVPVVPPAVARVAYPMATHNLHHELELVVAIGRSGANVAAADA
                     QALVFGYAVGLDMTRRDLQNEMREKKRPWDIGKSFAQAAPIAPISTVAQTGVLARGEV
                     TLRVNGETRQHGDLSDMIWDVPHMIEFLSRLYRLEPGDLIFTGTPSGVGPVVAGDRLE
                     GRIANLTPLSIEIVAPKD"
     gene            complement(12149..12793)
                     /gene="maiA"
                     /locus_tag="VFO53_11925"
     CDS             complement(12149..12793)
                     /gene="maiA"
                     /locus_tag="VFO53_11925"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HET8877030.1"
                     /translation="MRLYDYFRSSAAYRVRIALNVKGIVPDERTYVHLRMGSQRAQDY
                     LALNPQGLVPALELDDGSVLAQSLAIIEYLDETHPEPPLLPADPVARARVRAIALSIA
                     CEIHPLNNLRVLNYLIGTMGVSREQKDGWYRYWIDVGFEALEKTLARDAATGRFCHGD
                     APTLADVCLVPQMANARRFEIDLSPYPTLIRIESACNALPAFAQAAPARQPDAE"
     gene            complement(12813..14009)
                     /locus_tag="VFO53_11930"
     CDS             complement(12813..14009)
                     /locus_tag="VFO53_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET8877031.1"
                     /translation="MNAPEPTATRKPGFRWEDPLLLDLQLSDEERMVRDSAHDYAQER
                     LMPRVLDAFRHEASDPSLFAEMGALGLLGSTLPPEYGGAGLNHVCYGLVAREIERVDS
                     GYRSMMSVQSSLVMYPIYTYGSEVQRRNYLPSLASGERIGCFGLTEPNHGSDPGSMET
                     RAREVDGGFRMHGNKTWISNAPIADVLVVWAKLDGVVRGFILEKGMKGLSAPAIEGKF
                     SLRTSVTGEIVMDDVFVPADNLLPNVEGLKGPMGCLDSARYGIAWGALGAAQFCWEAA
                     RRYVLDRKQFGRPLAANQLVQKKLADMQTEIALGLQGCLRLGRLKDEHRASPEITSIM
                     KRNSCGKALEIARTARDMHGGNGIVDEFHVIRHMLNLETVNTYEGTHDIHALILGRAQ
                     TGISAF"
     gene            14156..14683
                     /locus_tag="VFO53_11935"
     CDS             14156..14683
                     /locus_tag="VFO53_11935"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HET8877032.1"
                     /translation="MRFAIHRLHAFTLVELMIALAIAGIVGALAVPAFGTWLAGQQVQ
                     GRAEALLYALDLARSEAIKRGDRVDVCPGDGGCDAGALAWEGGWVVVPDTVRRNASSA
                     IGRERTAPAGVTIRGNRPVADYVSYTGAGRARRFDGALQMGTFTVCRAGQRARKVILA
                     NSGRARIELSPDTCP"
     gene            14680..15120
                     /locus_tag="VFO53_11940"
     CDS             14680..15120
                     /locus_tag="VFO53_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HET8877033.1"
                     /translation="MTRSASPGFTLLETMIVVAIVAVLAAIAVPSYAGYVKRSHILEA
                     VARLSDARARMEDYFQDERTYIDGTGRCGAAPPAIGSADAFTLSCTATATTYTYTATG
                     SAAKAMSGFVFTVDQTGARSTVSVPPGWRRTTDCWTIRVDGLCA"
     gene            15117..15536
                     /locus_tag="VFO53_11945"
     CDS             15117..15536
                     /locus_tag="VFO53_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877034.1"
                     /translation="MNRSASRGAFLLEALVALIVLSLGLLGVLGLLAGAMHASGSAQW
                     RSEGFDVAAAALSRIFTDEPAGLAARYDASVDGPGYRELLVQAMRLPGVSSDVNAPIV
                     SIVDSAESRRIRVIVHWQLPGEAASHKASVSAVLPHR"
     gene            15671..16654
                     /locus_tag="VFO53_11950"
     CDS             15671..16654
                     /locus_tag="VFO53_11950"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HET8877035.1"
                     /translation="MLSLVIVYQAFVATDAVRRNAATAADAHGSAAFALYTLAAQITG
                     AGAGWVSAARALDTCPAIADMATTLRPIAVLITEGGGANRPDSLVVRQTFARRVALPV
                     AVAAAADAGTHVRAQSPDGFTAGDRIVAVTRTGNCVTTQVSAVAAPSAGIVDLTHTGI
                     AIDLPITTVLVDLGAASRASTLRFDVLAETLRSQDLANGDAPVPLTSNVVNLKFQYGI
                     DSNGDGALDTWARADAAGAWSPAALLAAPRATLERIKAIRVGIIVRTERVDRTLARDF
                     HWVLFDCALDDKAACPGRLEGTIPGSANGSYRYRVLETVVPLRNVIWNRSG"
     gene            16658..17251
                     /locus_tag="VFO53_11955"
     CDS             16658..17251
                     /locus_tag="VFO53_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877036.1"
                     /translation="MKRHARGVILLVAMVAVVALAFAGMALVRAVATSAAIGGNVNAR
                     QQAIFAASVALERDVVALFEGGGIDTTNDDPPHNYFASRQAGEDRRGVPAMLQSPADY
                     PAGAGIADAGDGFTVRHVIERLCLLPGAASIENCTLSPPSVEAASGAPPAAEPVRQPA
                     YRVTMRVEGPAGAATFVQAMLSAAHDNPRLTWQVLDE"
     gene            complement(17304..>18059)
                     /locus_tag="VFO53_11960"
     CDS             complement(17304..>18059)
                     /locus_tag="VFO53_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8877037.1"
                     /translation="LGGAKNHMVVMPDADLDQATDALMGAGYGSAGERCMAISAVVAV
                     GDVADSLVDRLAKRARKVQTGPGHAPDSEMGPVITCAARDRITGLIDRGVEEGASLVV
                     DGRKPAVDGHANGFFVGPTLFDHVKPYMTIYREEIFGPVLEVLRVDSLTGAIDMINAN
                     PYANGAAIFTRSGHAARRFEQDAEVGMAGINIPIPVPMSFYSFGGWRNSLFGDLHVHG
                     LDGVRFYTRLKTVTSRWPDDGSAAPGFHMPVMG"
BASE COUNT         2855 a         6220 c         6046 g         2938 t
ORIGIN      
        1 gcgaagacgc tcgatccgcg ggccgggact gcgactatcg aacggcgcga ggttcgtcag
       61 cgaacggcat cgacggcgcg gcgtgatgga cgcttgaccg ccgggatgcc ggacactccg
      121 cgggatgtgg ccttcgcgag cgtggcgatc gcgttcccga gtccgcgcag gcgctcaagc
      181 tgctcgggct cgtctgttcg gctcggcttg agctacggca gctaatcagt ccgtcacgga
      241 aggacacctc gggttgcggc tcgggcgccc tttttttgtc agcggcgctc gtagagcagc
      301 atctgcgtgc agcggaagag cgcgatcgtc cgcgcatcgc gcacgcgcgt gacggtcgca
      361 tcccagacct gcgtggtggc gccgagatga accgcgcgcg ccgagcacgc gatcgtgccg
      421 tccttggcgg tcccgagaaa attcgccttg agctcgatcg tcgtgaagtt gacggcgccg
      481 ccgggaagat gcgcaagcga gccgtaaccg cacgcggtat ccgcaagggc gacgatcgtc
      541 gccgcatgca ggtagccgtt cggcgcgcac agccgcggct gcacgtcgag ctcggcgtcg
      601 agatgtcctt cgcgaagcgc aatcacccgc agcccgacga gtcccggcag caatccgtcg
      661 ccgcggcggt tcagcgcgtc gatcgtgcag tccaggcgca gcgtcatgcc ggccttccag
      721 gttttcagcg cgcgtgcgcg agcacggccc gtacatcgtc gacgaagcga tcgacgacct
      781 cggccggcgt atcccacgcg cacatcagcc gcacgccggt gtcgccgacg aagacgtaga
      841 agcgccagcc cttgccgtgc agcgcatcga ttgcgtgctt cggcagatcg acgaacacgc
      901 cgtttgcttc cacgggtgca agcagtcgcg cctcgcgaag cgtggacagg ccgtgcgcaa
      961 gacgctttgc cattgcgttc gcatgggcgg cgtgggcaag ccacgcgcca ttctccagca
     1021 cgccgagcca cggtgcggcc agaaagcgca tcttcgatgc gagctggccg gcctgcttgc
     1081 gcctgcgcgc gaattcgtcg gccagcgcgc gatcgaagaa caggatcgct tcgccggcgg
     1141 gcagcccgtt cttcgtccca ccgaggcaca ggacgtcgac accggcgcgc cagctcgcat
     1201 cggccggact gcagtcgagc gacgcgaccg cattcgcgaa gcgtgcgccg tccatttgca
     1261 cccgcatccc gcgtccgtgg gccgtgtcgc acagggcggc gagctccccg cgcgtataga
     1321 cggtgccaag ctcggtggcc tgcgtgagcg agaccgcgcg gggccgcgac gaatgctcgt
     1381 cgtgcggcgt gacggatttt gcgatcaccg cgtcgggcgt gagcttcgcg agcggcgcgt
     1441 ccacggtcag aagccttgcc ccgccgctca tgaactcggg tgccccgcat tcgtcgacat
     1501 tcacgtgggc cagcgcgtgg cacacgatcg cgtcggtatt gcggcacaac gccgcgagcg
     1561 caagcgaatt cgccgcggtg ccgttgaaca cgaaaaaaac atcgcagtcg gtttcgaacg
     1621 tccgctggat ggcgtcgatc gcggcgcatg tccagtcgtc gccaccgtag ccgggtgcgt
     1681 ggccgcggtt ggccgcttcg agcgcgcgcc acgcctcggg gcagatgccc gcgtagttgt
     1741 ccgatccaaa ctgcggcgat agcgcctcga tgctcatagt cggtcgccga cccagccgct
     1801 caccgactgc agcgccttcg gcagcgcggc cggattcgag ccaccggcct gcgccatgtc
     1861 ggcgcgtccg ccgcccttgc cgccgacctg ctgcgcgacg tggttgacga gatcgcccgc
     1921 cttgaccttg ccggtcaagt cgctggtgac gcccgcaatg agcgtcacgc ggccgtcggc
     1981 gacgctcgcc agaacgatgg ctgcgctctc gagcttgtcc ttcagcttgt cgatcgtttc
     2041 gcgcagcgcc ttcgcatcgc tcgcctcgag cgcggcggca accaccttca cgcccttgac
     2101 gtcgattgca ttgcccgcga gatcatcgcc cgcgctcgcg gcggccttcg ccttcagacg
     2161 cgcgagttcg cgctcggtgt tgcgcgcgct ttcctgcagt tgcgcgatgc gcgcctcgag
     2221 ctcggcgagc ggcgccttca gcaggcgcgc aacgccggcc agtttcgctt cctggtcctg
     2281 cacgtagtcg agcgcgcctt tgccggtgac cgcttccacg cggcggatgc cggccgcgac
     2341 gccgctttcg gaaacaatct tgaacaggcc gatgtcgccg gtgcgcgcga cgtgcgtgcc
     2401 cccacagagc tcgcgcgagc tgccgatgtc gagtacgcgc acctcgtcgc cgtatttctc
     2461 gccgaacagc atcatcgcac cggatttctg cgcttcggcg atgggcatcg tgcgcgcgct
     2521 gacaccttcg ttcgcgagga tttccgcgtt gacgatggcc tcgacgcggc ggatctctgc
     2581 atcggtcagc ggagcatcat gcgcgaagtc gaaccgcgtc ttgccgggat cgacgagcga
     2641 gcccttctgc tgcacgtgcc cgcccagcac ttcgcgcagc gccttgtgca tcaggtgcgt
     2701 cgccgagtgg ttgcgcatcg tgcgtccgcg tgcctcgcag tccacggtgg cggcaacgcg
     2761 atcgcccgcg cgccattcgc cggtcttcat ctcgcccccg tgcccgacca cgtcgggttg
     2821 caccttctgc gcatcgtcga cggcgaagag cgtgaggcac gcgccgccct tcgaaagctc
     2881 gccgcgatcg ccgacctgtc cacccgattc cgcgtagaac ggcgtgcggt cgagcacgac
     2941 gatgccgcgc tcgccggtcg acagcgactc gacgcgcgcg ccctccttgt agagcgcgac
     3001 gacgcgtcct tcctcggaca gcgattcata gccgcggaac gccgtcttcg ggccgccgta
     3061 agccagcgct tcacccgact tgaactgcga ggcggcgcgg gcgcgctcgc gttgcgcgtc
     3121 catcgcgcgg tcgaagcccg ctgcgtcgac cgttatcccc gcctcgcgcg cgacgtcggc
     3181 cgtgaggtcc agcgggaagc cgtacgtgtc gtagagggtg aacgcggtct cgccatcgag
     3241 cacgcgcttg ccgtcgcgaa gtgcttcgcc gagcatgcgc atgccgttct cgagcgtttc
     3301 gccgaagcgc tcctcctcgg ccttcagcac ctgcgccacg cgcaccttgt cgcgcgcaag
     3361 tccgggatag gcttcaccca tttcgcgctc gaggtcatcg acgagccgga agaagaaagg
     3421 tcgcttctgc ccgagttggt agccgtggcg gatcgcgcgg cggatgatgc gtcgcaggac
     3481 atagccacgc ccttcgctcc ccggcatgac gccgtcgacg atgagaaagg cgcaggcgcg
     3541 gatgtgatcg gcgatcacct tgagcgacgg gctgtcgcga tcgctcgtgc gcgtctcgcg
     3601 tgcggcggcc tcgatcagcg tcgtgaagag gtcgatctcg tagttcgaat gcacgtgctg
     3661 cagcacggcc gcgagccgct cgaggcccat gcccgtgtcg acgcacggcc gcggcagcgg
     3721 agtcatcgtg aattcatcgc cacgcttctg gcggtcgaac tgcatgaaca cgaggttcca
     3781 gatttcgatg taacggtcgc cttcggcatc ggccgatccc ggtggcccgc cgaagacacc
     3841 cggaccgtgg tcgtagaaga tctcggagca cgggccgcac ggtcccgtat ccgccatctg
     3901 ccagaagttg tcgctcgcgt acctcgcacc cttgttgtcg ccgatgcgaa tgcagcgttg
     3961 cgccggcacg ccgatgtcgc gcgtccacag gtcgtacgcc tcgtcgtcgt cgatgtagac
     4021 ggtgacccac aggcgctcgg cgggcaggcg atagtgctcg gtgaggagtt gccacgcgta
     4081 gcggatcgca tcccgcttga agtaatcgcc gaagctccag ttgcccagca tctcgaagaa
     4141 tgtgtggtgc cgggccgtgt agccgacgtt ctcgagatcg ttgtgcttgc cgcctgcgcg
     4201 gaggcacgcc tgcaccgaag tggcgcgaac atagggccgc gtgtcggtgc cgaggaacac
     4261 atccttgaat tgcaccatgc ccgaattggt gaagagcagc gtcgagtcgt tggccgggac
     4321 gagcgacgag ctcgggacgt gcgtatggcc cttcgatacg aagtaatcga tgaactggcg
     4381 gcggatgtcg gcgactttca ttgcgggggg ctcgtgcgct ggatgcccgg cgccaaaaag
     4441 cgccagaaca tcgtttaatc aaaagcttac tagtataacc gcgaaccggt cgcacgcgct
     4501 cggaacgcga cgtaccgacg cgcttcagcc gtcgccgatg tccgtgccgg cctcgtgatc
     4561 ggtgcacgcg tcggcgcacg agcgtccgac gaccgccagc gcaaccgcga caccgtaacc
     4621 gcgcgcaatg agaaagcgga tctggcgcgc gcgttcgcgc tcgtcaccgg gagcgctccg
     4681 gaaacgtcga gcccacacgg cacgcgcgag cgccagctcg tcggaacctg cgagcgtatc
     4741 gagcgccgcg cgcgccgcag tggcctcgac gcgctgtgcg cgcatcgcat gcgcaatggc
     4801 gcgcttgccg aagcgagcgg acttctgtgc gaccagcata tgcgcgaacc gcgcgtcgga
     4861 caggtaaccc agccgctcga gctcgtcgag cgtgcgcgtc acttcggcgg gatcggcacc
     4921 gcgccccgcg agccgctgct cgagcgccgc ccgcggatag tcacgccgcg ccagcatgcg
     4981 gattgcggtg gcgcggacgg aagcgatcgg tttgcggcga gcgtcgcgca acgagtctcg
     5041 cgttttgccg agatggaaag gcgttacacg tcggcgccgt tgccgtgact ggcgccgttg
     5101 cccttgatcg gcggcggcga gggcacgccg accgcctcgc ggattttggc ttcgagctcc
     5161 ttcgccatgt cgggatgctc gcgcaggaac tcgcgcgcgt tgtccttgcc ctgcccgatc
     5221 ttgtcgccgg cataggcgta ccacgcgccc gacttctcga tgattcgctg gttgacgccg
     5281 agctcgatga tctcaccctc acggctgatg ccctcgccgt aaaggatgtc gaacagcgcc
     5341 tcgcgaaacg gcggcgaaac cttgttcttc accaccttca cgcgcgtttc gttgccgacc
     5401 acctcgtcgc cgcgcttgat cgagccgatg cgccggatgt cgagccgtac cgacgcatag
     5461 aacttgagcg cattgccacc ggtggtggtc tcggggttgc cgaacatgac gccgatcttc
     5521 atccggatct ggttgatgaa gatgacgagc acgttggcgc gcttgatgtt cgacgtgagc
     5581 ttgcgcagcg cctggctcat gagccgtgcc tgcaggcccg gaagctggtc gcccatttcg
     5641 ccttcgattt cggccttggg cacgagcgct gcgaccgaat cgatcacgat gatgtcgacg
     5701 ccccccgagc gtacgagcat gtcggcgatc tcgagtccct gctcgccggt gtcgggctgg
     5761 gaaatcagca gctcgccgac gttgacgccg agcttgccgg cgtagacggg atcgagcgca
     5821 ttttcggcgt cgatgtatgc ggccgtgccg cccgccttct ggacctgtgc gacgacctgc
     5881 aggcagagcg tggtcttgcc ggacgattcg ggaccgtaga tctcgaccac ccgtccgcgc
     5941 gggaggccac ccacgccgag cgcgatatcg aggccgagcg agccagtgga gacgacggtc
     6001 aggtcgttct ggatctgggc ctcgcccatt ttcatgatcg agcccttgcc gaactgcttc
     6061 tcgatctgcg cgagcgccgc ggcgagcgcc ttgaccttgt tgtcgtcgct ggtttgtccg
     6121 gaaatcatcg tcgtctctcc tcgaaagtgg cgcgattatg gcacgaacga tcgttcgatg
     6181 gaagcggtcg aaccgcctag gcggcctcgc cgattgacgc cactgctcgg tctgcaacgc
     6241 atcagcgcga cagcggcagc cgcgtgtcga gcagctcgat caatccggcg agcgccgcct
     6301 cgaccgccgc cgcccggatg ccaccgcgcg tcccgtcgaa atgccgcgtc atgacctcgg
     6361 tcggcggcgt cggcaacgtc cagccgaaac acaccatgcc caccggcttc gctgcgcttc
     6421 cgccacccgg gccggcgatc ccggtgaccg agatggtgca gtcggcatgg cttctcgcca
     6481 gcgcaccgtc ggccatcgca cgcgcggccg cttccgagac cgcccctgcc gtttcgagca
     6541 cgtcgcgcgg aacgcccaga agctcgcatt tcgcgtcgtt cgaatacgtg acgaatccgc
     6601 gctcgaacca ttcggagctc ccggcaatgg ccgtcatcgc cgcagcaacg agtccgccgg
     6661 tgcacgattc ggcggtggcg acgcgcatgc cgcgcgcgag caacgcgtgt ccgagcgcca
     6721 tcgcgagccg ttcctgggtc tcctctatcg acattcgctc acccgtgcac ggtaatgaca
     6781 ggtatacgcc gtcgcgaagc cggccttgcg atagacggcg agcgccgcgg cgaggcacgc
     6841 agcgcaccga ccgactcgac gagcgctgcg gtcggccgcc aatgagtcga catgcgtcgg
     6901 catcagcttc cgagcaatcg ctcgcccacc gcgaggacca gcagcgtgta gccggcagcg
     6961 gcgaaatcgt cggccatcac gccggcggca ttcttcatgg cgcggtcgat ctcgcggatc
     7021 ggcggcggct tgacgatgtc gaaaaagcgg aagagcacga aggcaagtgc ctggcgccat
     7081 ggcgcagcgc ccgcgaagaa cagcacgaga aggaaggcgg cgatctcgtc gatcacgatg
     7141 ctgccatgat cgggcacgcc gagatcgcgc gccgtgatgt gcgcgcacca tgcgccggcg
     7201 atcgtgatcg cggcgatcgc caccagccat ccccaggtcg ccgcatgcgc gtcgagaagc
     7261 cacgcgagcg gcaatgcgac gagcgtgccg aaggttcccg gggcgaccgg tgaaaggccc
     7321 gtgccgagcc cgagcgcgat gaagtgcgcc ggatgacgga cgaggaacgc aaacgtcggc
     7381 gccttcatcg tcgccgtttg catcgcatca ggcgaaatgg tcgaacgcgt gcggcagcga
     7441 cgccatcggc gtgccgtgct cgtcgcggat cgcgaggccc ggctcggccg tgatcgcgcc
     7501 gatcggggtc agaggcaccg acagcgcatg cgcgagcgca tcgacccgct cgcgcatcat
     7561 gggtgtcgca gtgaagcaca actcgtagtc gtcgcctccg gcaagcaggc atgcaagggc
     7621 gagcaggcgc tgcggcccgc gcgccagcgc acgcaacgca tcgctgcagg gaatcgcggc
     7681 gagatcgatc gtcgcaccca cgcgcgacgc gtcgaggatg tggcgaagat cgccggtgag
     7741 cccgtcggac acgtcgagcg ccgcggatgc gatgccgcgc agcgcaacgc cgagtgctat
     7801 gcgcggctcg ggcgcatcca ggcgacgctg caatgcgggc agcagcgtcg ggtcgatctc
     7861 gacccgcctg cctgcgacgg ccagcgcaag cgccgcatcg ccaaggcggc cggacacgaa
     7921 gatcgtgtcg cctggattcg cgccgccacg cgtgatcgcc gtgccggccg gcagctcgcc
     7981 gatcgcggta acgcagaacg ttcgcgggcc gcgggtggtg tcgccgccga tcagctcgac
     8041 gtcgtactgc gcggcgagcg cgtcgaagcc gcgcatgaac gcctgcagcc aatcggggtc
     8101 ggcgaccggc aatgcgccgg caaggagcac gaagcgcggc aacgcaccca tcgcggcgag
     8161 gtcggacaga ttgacggcga gcgtcttgtg tccgagctgg cgcggatcgg cgccggcggc
     8221 gaagtgacgg ccctcgacga gcatgtcgac cgtcagcacg tattccattc cggctgcggg
     8281 tgcgacgacg gcgccatcgt cgccgatgcc gatgcgcaca tcgccgtttc gcggcggacg
     8341 gcggaagtag cgctcgatca cttcgaactc gttcatcggc ggccggagac gcggatctcg
     8401 tcggcgcgtt cccgtgccgc gaccttgtcg aggacgccat tgacgaactt gtacccgtcg
     8461 gtgccgccgt agcgcttggc gagctcgacc gcttcgttga tcacgacgcg gtacgggatg
     8521 tcgagacggt gcgccagctc ccacgcgccg atcgtgatca ctgcgcgctc gatgggcgca
     8581 agcgcgtcga tgccgcggtc gacgtgcggc gcaagctgcg cgagaagcgc gtcccattct
     8641 ccggtgacgc cacgccacaa ctcgtcgaaa tacgcggcgt cgaaaccggc agtgtccgcg
     8701 gcgtcggctt cttcggccgc ggcatggccg ggctcggcga tcagcgacgc ggcgatgtcg
     8761 cgatcgggac gccgggacat ctgtcgctcg tagattccgt gcacgacgag ctcgcgcgca
     8821 cggtggcgtc ccgcatgctc gctgccgcta ccggccatcg atcgcgtcga gcaggttggc
     8881 gagctccagt gccgcgagcg cggcttcact gcccttggcc gccatccgcg cctcggcctg
     8941 tgcatcggtg tcgcaggtga ggatgccgtt gccggtcggc acgccgaatt cgagctgcac
     9001 gctcgccaca ccggccgccg attcgttcgc gacgatctcg aaatggtagg tatcgccgcg
     9061 gatgaccgcg ccgatggcca ccagaacgtc gtggtcgccc gattgcgcga gccgctgcag
     9121 cgcgagcggg atctcgaggg caccgggaac ggtgatgagc gtgatgcgct cctcggcgac
     9181 gccggcctcg tgcagcgccc gcaacgcgcc cgcgagaagc ccttcgccga tacgcgggtt
     9241 gaatcgcgat tgcacgatgg cgacgcggcg ctcgtcgccg cgcagattcg gttcgatgcg
     9301 gaggatcggc attgcaaatg agggaagacg ggctggccgc cggcgcgccg ccgggcgtcg
     9361 gtcggcgcta cgatgcccgg cgtgcgggcg cttcctcgta gcccgtcact tcgagatcga
     9421 agccggtcat gctcggcatc ttgcgctgcc gcgcgaggag ccgcatccgg ccgacgttga
     9481 ggtcgcgcag gatctgcgcg ccgatgccgt aattgcgcaa atccatcttc gacgatgccg
     9541 gcacgtcgcc ggcaacggcg cgctggcgca gctcctgcgc gctttcggga cgatgcaaaa
     9601 ggacgatcat gccgcgcccg gccgccgcga tcgcgtgctg tgcctcgagg atgctccagg
     9661 cgtgcgagtc gctctcggaa tcgagcaggt cgacgaccga caggggctcg tgcacgcgca
     9721 ccagcgtttc gacgtcgggc gagatgggcc cgcgcgtcag cgcgagatgc gtcgcatcgg
     9781 tcagcttgtc gcgatagacg acgagcctga acgtcccggc tggcgtgcgc agcgggcgct
     9841 cggcaacacg ctcgatcagc cgctcggtgc gactgcgatg ctggatgaga tcggcgatcg
     9901 cgccgatctt caatccgtgc gcggatgcga acacctcgag atcgggcagg cgcgccatcg
     9961 tgccgtcgtc gttcagtatc tcgcacagca cggccgacga cgaaaggccc gccagctggg
    10021 cgagatcgca gcacgcctcg gtatgtccgg cacgcacgag cacgccgccg ggttgcgcaa
    10081 tcagcggaaa gacatgcccc ggctgcacga tgtcgtcggg cgtcgcatgc gctgcggctg
    10141 cgacgcgaat cgtgtgcgcg cggtcggcgg ccgagatgcc ggttgccacg ccgtgtgccg
    10201 cttcgatcga cacggtgaaa ttcgtcccgt gcaccgagcg gttcacacgc gtcatcggcg
    10261 cgaggtcgag ctgccgcgcg cgccgctcgg tgatcgccat gcacacgagg ccgcgtccga
    10321 agcgcaccat gaaattgacc gcttccggcg tgacgtggtc ggcggcgagg acgagatcgc
    10381 cttcgttctc acggtcctcc tcgtcgacga gcacgaccat ccgcccggcg cggatttcgg
    10441 caacgatttc gacgatcggc gcaatgggca tgttttcaac cggccacggc gagcggcatt
    10501 cgcggcattt taccgtagcg gctcgactcg catcaccgaa cgcgcgcccg cgagcgcagg
    10561 cggtcaggac ccgaacagcg caacggtggc cttcgtgagc ctgctcgccg gatcggcgta
    10621 gtcgagcacg acgctgtagt gattgcgtcc ggcaacgtgc atgggctggg caacgccgcg
    10681 cgcgcggttg cgcggccacg cttcccacag gagatcggtc tgccgaacga actcggaggt
    10741 ctcgtcggcg cccacggcga gcagcagcgg cacatcggtt gtcggggtcg ccatggccgg
    10801 cgacatgcgg cgtgcctcgg cgtcgtcgag cctgaagtcg acgttgtgcg agaactgcac
    10861 cagcggcgcg agctcgtgca cgccggaaag cgacgcgccg ccaacgaacg gcgcatcggc
    10921 gagaccgtgc gcacgccagt cgatcgcgta catcatcgcc gtcagatgac cgccggcgga
    10981 atgaccgccg atcacgatcg gccccggcgc cccgcgcgcc ggtccttcgc gcacgagcca
    11041 cagcagcgcc cggcggcact gctcgacggc gttggcgatc gtgtcgcgcg ggcacagcgt
    11101 gtagttgacg agcgccaccg cgaaccccgc gtcgacgaac gccggcgcga cgaagccgta
    11161 gtccgacttg tcgaggctgc gccaccagcc gccatggatg tacatcagcg ttccacgtgc
    11221 cgccttcgtc ggcacgtaaa gatcgagcgt ttcgtcggcg ccgctgccat aggccaggtc
    11281 gatcgccggc gacagcgcct gctgcgcagt cgcggagcgt gcgatccact cgtcgagcca
    11341 gcgctgatgg tcgggaactg cggcgcgatt gttgtagccg cgctcgatct cgtcaggcgt
    11401 catcgcaggc gtcggcgcca tcgatcaatc tttcggggca acgatctcga tcgaaagcgg
    11461 ggtcagattc gcgatgcgac cctcgagccg gtcaccggcc accacgggac ccacccccga
    11521 cggcgtaccg gtaaagatca ggtcacccgg ctcgagccgg tagaggcgcg acaggaattc
    11581 gatcatgtgc ggcacgtccc agatcatgtc cgacaggtcg ccgtgctgcc gcgtctcgcc
    11641 attgacgcga agggtgactt cgccgcgcgc gaggacaccc gtctgcgcga cggtcgagat
    11701 cggcgcgatc ggcgccgcct gcgcgaacga cttgccgatg tcccacggac gcttcttttc
    11761 gcgcatctcg ttctgcaggt cgcgccgcgt catgtcgagc ccgacggcgt agccgaacac
    11821 gagcgcctgc gcatcggcgg ccgcgacgtt ggcaccgctg cgcccgatgg cgacgaccag
    11881 ctcgagctcg tgatgcaggt tgtgcgtggc catcggataa gcgacgcgcg ccacggccgg
    11941 cggcacgacc ggcacgatcg catcggccgc tttggtgaag aagaacggcg gctccttcgt
    12001 cgcgtcgccg cccatctcct tcgcatgctc cgcgtaattg cggccgacgc aaaacacgtg
    12061 acgcaccgga aactgccgtt gacctccggc gacggggacc gcgggcagat cccaggccgg
    12121 gatggcgtat tgcatggtca atggctcctc attcggcgtc gggctggcgc gccggcgccg
    12181 cctgcgcgaa cgccggcagc gcgttgcacg ccgattcgat gcggatcaac gtgggatagg
    12241 gggacagatc gatctcgaag cggcgcgcgt tcgccatctg cggcacgagg cagacatcgg
    12301 caagcgtcgg tgcatcgccg tggcagaagc ggcccgtggc cgcgtcgcgg gcgagcgtct
    12361 tttcgagcgc ctcgaagccg acgtcgatcc agtagcgata ccagccatcc ttctgctctc
    12421 gcgacacacc catcgtgccg atcaggtaat tcagcacgcg caggttgttc agcggatgaa
    12481 tctcgcacgc gatcgacagc gcgatcgctc gcacgcgtgc ccgcgcgacc ggatcggccg
    12541 gcaggagcgg cggttcggga tgcgtctcgt cgaggtactc gatgatggcg agcgattgcg
    12601 caagcacgct gccgtcgtcg agctcgagcg ccggcaccag gccctgcgga ttgagtgcca
    12661 ggtaatcctg cgcgcgctgg ctgcccatgc gcaggtgcac gtacgtgcgc tcgtcaggca
    12721 cgatgccctt gacgttgagc gcgatgcgca cccggtacgc ggccgacgag cgaaagtaat
    12781 cgtagagtcg catcgcggtt ccggacgctc gatcagaacg ccgagattcc ggtctgcgca
    12841 cgcccgagga tcagtgcgtg gatgtcgtgc gtgccctcgt acgtgttgac cgtctcgagg
    12901 ttcagcatgt ggcggatcac gtggaactcg tcgacgatgc cgttgccgcc gtgcatgtcg
    12961 cgtgcagtgc gcgcgatctc gagcgccttg ccgcagctgt tgcgcttcat gatcgacgtg
    13021 atctccggcg aggcgcgatg ctcgtccttg agccgcccga ggcgcaggca cccctgcagc
    13081 ccgagcgcaa tttccgtctg catgtcggcg agcttcttct gcacgagctg gttcgccgcg
    13141 agcggccgtc cgaactgctt gcggtcgagc acgtagcggc gcgccgcttc ccagcagaac
    13201 tgcgccgcgc cgagcgcacc ccaggcgatg ccgtagcgcg cgctgtcgag gcagcccatc
    13261 ggccccttga gaccttcgac gttcggcaac aggttgtcgg cgggaacgaa cacgtcgtcc
    13321 atcacgatct cgccggtgac cgacgtgcgc agcgaaaact tgccttcgat tgcgggcgcc
    13381 gaaagcccct tcattccctt ttcgaggatg aagccgcgca cgacgccgtc gagcttcgcc
    13441 cagacgacca agacgtcggc gatcggcgcg ttggaaatcc aggtcttgtt gccgtgcatg
    13501 cgaaagccgc cgtcgacctc gcgtgcccgg gtttccatcg aacccgggtc cgatccgtgg
    13561 ttcggttcgg tgaggccgaa gcagccgatg cgctccccgg acgcaagcga aggcaggtag
    13621 ttgcgccgct gcacttcgct gccgtaggta tagatcggat acatgacgag cgagctttgc
    13681 acgctcatca tcgagcgata gccggaatcg acgcgctcga tctcgcgtgc gacgagtccg
    13741 tagcacacgt ggttgagtcc cgcgccgcca tattcgggcg gcagcgtcga cccgagcagc
    13801 ccgagcgcac ccatctcggc aaagagcgac gggtcgctcg cctcgtggcg aaacgcgtcc
    13861 agcacgcgcg gcatcagccg ctcctgcgcg taatcgtgcg cgctgtcgcg caccatccgc
    13921 tcctcgtcgc tcaactgcag atcgaggagc agcggatcct cccagcggaa gccgggtttg
    13981 cgcgtggcgg tgggctcggg ggcgttcatg cgcggggctg tgtgcgagtt cgccgattat
    14041 cgccgttcga cgatgcaatt gccaatgcgc accgcggcgt cattgccaat agcgcgaacg
    14101 gcctaggtca tctcgccaat atcgccgtct gcagggcctt cgcacaatcc tccgcatgcg
    14161 attcgcgatc caccgcctgc acgctttcac gctcgtcgag ctgatgatcg cactcgccat
    14221 cgcgggcatc gtgggcgcac tcgccgttcc tgccttcggg acgtggctcg ccgggcagca
    14281 ggtgcagggt cgtgccgaag cccttctgta cgcgctcgat cttgcgcgaa gcgaggccat
    14341 caagcgcggt gaccgtgtcg atgtctgtcc cggtgacggc ggctgcgatg cgggcgccct
    14401 ggcgtgggaa ggcggctggg tcgtggtacc cgacaccgtc cgccgcaacg cgtcatccgc
    14461 gatcggccgt gagcgcaccg cgccagccgg ggtcaccatt cgtggcaacc ggccggtcgc
    14521 cgactacgtg tcgtacaccg gcgccgggcg cgcgcggcgc ttcgatggcg cgctccagat
    14581 gggcacgttc accgtgtgtc gcgccggcca gcgcgcgcgc aaggtgatcc tcgccaacag
    14641 cggtcgcgcc cgaatcgaac tgtcgcccga cacctgccca tgacccgctc ggccagtccc
    14701 ggattcacgc tgctcgagac gatgatcgtc gtcgccatcg tcgcggtgct cgctgcgatc
    14761 gccgtgccgt cgtacgccgg gtacgtgaag cgatcgcaca tcctcgaagc ggtcgcgcgc
    14821 ttgtccgatg cgcgtgcgcg catggaggac tatttccagg acgagcgcac gtacatcgac
    14881 ggcaccggcc gctgcggcgc cgcgccgccc gcaatcggca gcgccgacgc attcacgctt
    14941 tcgtgcaccg ccacggcaac gacctacacg tatacggcaa cgggcagcgc cgcgaaggcg
    15001 atgagtggct tcgtcttcac cgtcgatcaa accggcgccc ggtcgaccgt ttcggtaccg
    15061 cccggctggc ggcgcaccac cgattgctgg acgatccgcg tcgacggcct ctgcgcatga
    15121 accggtccgc cagccgaggc gcgttcctgc tcgaggcgct cgtcgcgctg atcgtgctgt
    15181 cgctcggcct gctgggcgtg ctcggccttc tcgccggcgc catgcacgcg agcggaagtg
    15241 cgcagtggcg aagcgaagga ttcgacgtcg cggccgcggc attgtcacgc atttttaccg
    15301 acgaacctgc cggcctcgcc gcacgctacg atgcatcggt cgacggcccg ggatatcgcg
    15361 agctgctcgt gcaggcgatg cggctgccgg gcgtgtcgtc cgacgtgaac gcgccgatcg
    15421 tcagcatcgt cgacagcgcc gaaagccggc gcatccgcgt catcgtgcac tggcaattgc
    15481 ctggtgaggc ggccagccac aaagcgtcgg tcagcgccgt gctgccgcat cgctgaacat
    15541 gcgcggcatc atcgccttca ctcgcaacgc gtggcacggc gccgcggccg tcggctcgca
    15601 tcgtcccgcg tgccgaaggc ggctacgcgc cgcgtcgctg gttgacgcgc tggtcggcct
    15661 cgcagtcgcc atgctgtcgc ttgtcatcgt ctaccaggcc ttcgttgcca ccgacgcggt
    15721 acggcgcaac gcagcgacgg ccgccgacgc gcatggcagc gcggcgttcg ccctctatac
    15781 gctcgccgcg cagatcacgg gcgccggcgc cggatgggtg tctgccgcgc gcgcgctcga
    15841 cacctgtccg gcaatcgccg atatggcgac cacgcttcgc cccatcgcgg tccttatcac
    15901 cgagggcggc ggtgccaacc gccccgacag tctcgtggtc cggcaaacct tcgcacgcag
    15961 ggttgcgctg cctgtggccg tcgctgccgc cgccgacgcc ggcacgcatg tccgcgcgca
    16021 aagccccgac ggcttcacgg ccggcgatcg aatcgtcgcg gtcacgcgga ccgggaattg
    16081 cgtgacaacc caggtgagcg cagtcgcagc cccgagcgcc ggcatcgtcg acctcacgca
    16141 tacgggcatt gcgatcgatc tgccgatcac gaccgtgctg gttgacctcg gcgccgcgag
    16201 tcgcgcatcg acgcttcggt tcgacgtcct cgccgagaca ctgcgaagcc aggatctcgc
    16261 caatggcgac gcgcccgtgc ctctcacgtc gaatgtcgtc aacctcaaat ttcaatacgg
    16321 catcgacagc aacggagacg gcgcactcga cacctgggcg cgcgccgatg cagcgggtgc
    16381 ctggtccccg gcggcgctgc ttgccgcacc gcgcgcaacg ctcgagcgca tcaaggcgat
    16441 ccgcgtcggc atcatcgtcc ggaccgagcg cgtcgatcgc acgcttgcgc gcgactttca
    16501 ctgggtgctc ttcgattgcg cgctggacga caaggcggct tgcccgggcc ggctcgaagg
    16561 aacgattccg ggcagcgcca acggcagcta tcgctaccgc gtgctggaaa cggtcgtccc
    16621 gctacgcaat gtcatctgga accgaagcgg atagccgatg aagcggcacg cgcgcggcgt
    16681 catcctgctg gtcgcgatgg tggcggtggt tgccctcgcc ttcgcaggca tggcgctggt
    16741 gcgcgccgtt gccacgagcg cagccatcgg cggcaacgtc aatgcgcgcc aacaggcgat
    16801 attcgcggcg tccgtagcac tcgagcgcga tgtcgtcgcc ctgttcgaag gcgggggcat
    16861 cgacacgacg aacgacgacc cgccgcacaa ctattttgct tcccgccagg ccggcgagga
    16921 tcggcgcggc gtgcccgcca tgctgcagtc gccggccgat tatcctgcgg gagcgggcat
    16981 cgccgacgcg ggcgacggct tcacggtgcg ccacgtgatc gagcgcctgt gcctgctgcc
    17041 gggcgccgcg agcatcgaaa actgcacgtt gtcgccgccg agcgtcgaag cggcgtccgg
    17101 cgcaccgccc gccgccgagc cggtgcgcca acccgcctac cgggtcacga tgcgtgtcga
    17161 agggcccgcg ggcgcagcaa cgttcgttca ggccatgcta agcgcagcgc acgacaaccc
    17221 gcggctgaca tggcaggtgc tggacgaatg accgatgcgg gcgacccttg caacgcaggt
    17281 ggttcgcgtc agcgccccgc gcgctacccc atcaccggca tgtgaaatcc cggagccgca
    17341 ctgccgtcgt cgggccagcg cgacgtcacc gtcttcagcc gcgtatagaa gcgcacgccg
    17401 tcgagcccgt gaacgtgcag gtcgccgaac agcgaattgc gccagccacc gaacgaatag
    17461 aacgacatcg gcaccggaat cgggatattg atgcccgcca tgcccacttc cgcgtcctgc
    17521 tcgaagcgtc gcgccgcgtg acccgagcgc gtgaagatcg cggcaccgtt cgcatacgga
    17581 ttcgcgttga tcatgtcgat cgcaccggtc agcgagtcca cgcgcagcac ctccagcaca
    17641 gggccgaaga tctcttcgcg gtagatcgtc atgtagggct tcacgtggtc gaacagcgtc
    17701 gggccgacga aaaagccgtt tgcatgcccg tcgaccgcgg gcttgcggcc gtcgacgacc
    17761 agcgacgcgc cctcctcgac gccgcggtcg atcaatccgg tgatcctgtc gcgcgccgcg
    17821 cacgtgatca cgggacccat ttcgctgtcc ggcgcgtgcc cggggccggt ctgcaccttg
    17881 cgtgcgcgct ttgccaatct gtcgacgagc gaatccgcga catcgcccac cgcaacgacg
    17941 gccgatatcg ccatgcagcg ctcgcccgcc gaaccgtagc cggcccccat cagcgcgtcg
    18001 gtcgcctggt caagatcggc gtccggcatc accaccatat ggttcttggc gccgccgag
//
LOCUS       DASSVX010000277         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1395424, whole genome shotgun sequence.
ACCESSION   DASSVX010000277 DASSVX010000000
VERSION     DASSVX010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395424"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1285
                     /locus_tag="VFO53_11965"
     CDS             59..1285
                     /locus_tag="VFO53_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET8877038.1"
                     /translation="MTAVNLTVNGKAVAADVEPRTSLADFLRDALGLTGTHLGCEHGI
                     CGACTLMIDSMPARSCITLAVACDGADVTTIEGHDEDEVMQALRAAFSREHALQCGYC
                     TPGMLASARDVVLRMPDAGENDIRVAMSGNLCRCTGYVGIVRAIQRVIEARAANGAAL
                     PSTRRTALGPVGSDRSPMPLEPSGSAHASSRAPAAASSGSAPTEPASRAMAAASSTDK
                     PQSTLRQSFCVDHPRDDVWAFFGRLPEVTTCLPGTSLSGTPTNDHVEPRMRIKVGPII
                     AEFEGVADAVRDPSNHAGTIRGSARDTRSGSMTRGEIRYVLREEQGATATRVDIEVGY
                     TLTGPLAQFGRSAIVEDIAARMTDTFARNLQARLDRGPGGPAQTGPPAALDAGSLVFS
                     VLWQRISGALRRLFHR"
     gene            1308..>2289
                     /locus_tag="VFO53_11970"
     CDS             1308..>2289
                     /locus_tag="VFO53_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET8877039.1"
                     /translation="MSHLALEPQIVDASSRRNALERLRQLNITLPTFSELADPERIPA
                     RRLAALADVDPNEPQAANLWRVHWFNDEARTGRVDVPGYLVLPEALTGVKTRIVVALG
                     GRFPMIGAHKVLAAYGCLVPRLVTGRFDPTLHKAIWPSTGNYCRGGVAISRILGCRGV
                     AVLPAGMSQERFAWLERWVADDSDIIRTPGTESNVKEIYDQCAELSRDPHNLILNQFA
                     EFNNYLVHYLCTGRALERVFDDMRETEPGLRLAAFVAATGSAGTIAAGDYLKSVHGTR
                     IAAVEAVECPTMLCNGYGEHNIQGIGDKHIPLIHNVMNTDLVVGVSDQSSD"
BASE COUNT          385 a          777 c          749 g          378 t
ORIGIN      
        1 tcgacaacca gattcacgtc gcggcattga agcgcgctgt cgagaacgcc ggattcgcat
       61 gacggcggtc aaccttaccg tcaacggcaa agccgtcgcg gcggacgtcg agccccggac
      121 gagcctcgcg gactttttgc gggacgcgct cgggctcacc ggcacgcatc tcggctgcga
      181 gcacggcatc tgcggcgcgt gcacgctgat gatcgactcg atgccggcgc gctcctgcat
      241 cacgcttgcc gtcgcctgcg atggcgccga cgtcacgacg atcgaaggac acgacgaaga
      301 cgaggtgatg caggcgttgc gcgcggcgtt ctcgcgcgag catgcgctgc agtgcggcta
      361 ttgcacgccg ggcatgctgg cttcggcgcg tgacgtcgtg ttgcgaatgc ctgatgccgg
      421 cgagaacgat atccgtgtgg cgatgagcgg caacttgtgt cgttgcaccg gttatgtcgg
      481 catcgttcgc gcgatacagc gcgtgatcga agcgcgtgcg gcgaacggcg cggcgcttcc
      541 atccacgcgc agaacggcgt tgggacccgt cggctcggat cgttcaccga tgccgctgga
      601 gccttccggg agtgcgcacg cttcgtcgcg ggcgccggcc gccgcgtcat ccggatcggc
      661 gcccaccgag ccggcgtcgc gcgcaatggc tgcggcgtcc tcgacggaca agccccagtc
      721 gacgttgcgc cagtcgtttt gcgtcgatca cccgcgcgac gatgtctggg cattcttcgg
      781 ccgcctcccc gaggtcacga cgtgtctgcc ggggacgtcg ctcagcggca cgccgacgaa
      841 cgaccatgtc gagccgagaa tgcgcatcaa ggtgggcccg atcatcgccg agttcgaagg
      901 cgtcgccgac gcggtgcgcg acccctcgaa ccatgccgga acgattcgcg gcagcgcccg
      961 cgacacgcgg tcgggctcga tgacacgcgg cgaaatccgc tacgtgctgc gcgaggagca
     1021 gggcgcaacc gccacccgcg tcgacatcga ggtcggctac acgctgaccg gtccgctcgc
     1081 gcaattcggt cgatccgcga tcgtcgagga catcgctgcg cgcatgaccg acacgttcgc
     1141 gcgcaatctg caggcacgcc tcgatcgggg cccaggcggc cctgcgcaaa caggcccgcc
     1201 ggccgcactc gatgccggct cgctcgtttt ctcggtgctg tggcagcgca tcagcggcgc
     1261 gctgcggcgc ctgttccatc gctgaacgcg aaaggaattc tcgtcgcatg agccatctcg
     1321 ccctcgaacc gcagatcgtc gacgcaagtt cgcgccgcaa cgcgctggaa cggctgcgtc
     1381 aactgaacat cacgctgcca acgttcagcg agctcgcgga tcccgaacgt attccggcac
     1441 ggcgactggc ggcgcttgcc gacgtcgacc ccaacgagcc gcaggcggcg aacctctggc
     1501 gcgtgcactg gttcaacgac gaagcgcgca ccggccgtgt cgacgtcccg ggatacctgg
     1561 tgctgccgga ggcgctcacc ggcgtgaaga caagaatcgt cgtggcgctc ggcgggcgct
     1621 ttccgatgat cggcgcgcac aaggttcttg ccgcgtacgg ctgcctcgtg ccgcgtctcg
     1681 tgaccggccg cttcgacccc acactgcaca aggcgatctg gccgtcgacg gggaactatt
     1741 gccgcggcgg agtggcgatc tcgcgcattc tcggctgccg cggcgtcgcc gttttgcccg
     1801 ccggcatgag ccaggagcgc ttcgcctggc tcgagcgttg ggttgcggat gattcggaca
     1861 tcattcgcac cccggggacg gaaagcaacg tcaaggaaat ctacgaccaa tgcgccgagc
     1921 tttcccgtga cccacacaat ctcattctca atcagtttgc cgaattcaac aattacctcg
     1981 tccactacct ttgcacgggt cgcgcgctgg aacgcgtgtt cgacgacatg cgcgaaacgg
     2041 agccggggct tcgtctcgcg gcgttcgtcg cggccactgg ctccgcgggg acgatcgccg
     2101 ccggggacta tctcaagtcg gtccacggga cgaggatagc ggccgtcgaa gccgtcgaat
     2161 gcccgacgat gctctgcaac ggctacggcg agcacaacat ccagggcatc ggcgacaaac
     2221 acattccgct catccacaac gtgatgaaca ccgacctcgt cgtcggcgtg tcggaccaga
     2281 gctcggatg
//
LOCUS       DASSVX010000278         7440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_262287, whole genome shotgun sequence.
ACCESSION   DASSVX010000278 DASSVX010000000
VERSION     DASSVX010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262287"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..567)
                     /locus_tag="VFO53_11975"
     CDS             complement(4..567)
                     /locus_tag="VFO53_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET8877040.1"
                     /translation="MAKPRAWGDPTRDEVARSNRTLLTKLGVVVVAMFGFGYALVPFY
                     EKICEVTGLRNIDSADTVTNTQVDTTRSVRIEFDTNLRNLPWQFRALQLATDVHPGAV
                     TQAMFEVVNTTDRPITGQAIPSYGPRQAALYFKKLDCFCFSQQTLAPHEKRDMPVVFV
                     VDPKLPKDVPTITLSYTFFEVEGHERS"
     gene            complement(656..859)
                     /locus_tag="VFO53_11980"
     CDS             complement(656..859)
                     /locus_tag="VFO53_11980"
                     /inference="COORDINATES: protein motif:HMM:NF038351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HET8877041.1"
                     /translation="MANEPQRDARLRAANRRTGLVLLAIALLFFFGIIASRFIESEMV
                     SIAVVGGAILLYLVVAIGRHLRK"
     gene            complement(906..2501)
                     /gene="ctaD"
                     /locus_tag="VFO53_11985"
     CDS             complement(906..2501)
                     /gene="ctaD"
                     /locus_tag="VFO53_11985"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET8877042.1"
                     /translation="MSSIPHDLVVGGVPHGEHAHHPKGIMRWITTTNHKDIGTMYLWF
                     SFTMFIVGGIMALTIRSELFQPGLQIVKPEFFNQMTSLHGLIMIFGALMPGAVGFANW
                     LIPMQIGAPDMAFARMNNWSFWLLPPAAIMLILSLFVPGGAAETGWTLYPPLSIQSGM
                     GMDLTIFAIHIMGASSIMGSINIITTILNLRAPGMTLMKLPLFCWAWLITAYLLIAVM
                     PVLAGAVTMLLTDRHFGTSFFNAAGGGDPVLFQHVFWFFGHPEVYIIILPAFGIISEI
                     VPTFSRKPLFGYASMVYALAAIAILSFLVWAHHMFTVGMPAASILFYMYATMLIAIPT
                     GVKVFNWLATMWKGSITLEAPMLFALGFLFLFVIGGFTGLILAIQPVDVQFQDTYYVV
                     AHFHYVMVAGTLFAFFGGTYYWLPKWTGHMYDEKLADWHFWLSFIFFNVAFFPMHFLG
                     LAGMPRRVPDYAQQFTDWNMVSTIGAFGFGLSQLLFLATVVKCIRGGAPAPAKPWDGA
                     TTLEWTLPSPAPYHSFETPPVVK"
     gene            complement(2543..3577)
                     /gene="coxB"
                     /locus_tag="VFO53_11990"
                     /pseudo
     CDS             complement(2543..3577)
                     /gene="coxB"
                     /locus_tag="VFO53_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009970051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
     gene            complement(3845..4531)
                     /gene="bioD"
                     /locus_tag="VFO53_11995"
     CDS             complement(3845..4531)
                     /gene="bioD"
                     /locus_tag="VFO53_11995"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HET8877043.1"
                     /translation="MNRTAASSRGIFVTGTDTGVGKTRIATALLHALARAGVRAVGMK
                     PVAAGFEPAANINGDVIALRAAGNVDVPLADCNPYAFADAVAPHLAAAAAGIDIAMPA
                     IVGAAGRLRERADALVVEGAGGALVPLDGTRDMLDIAAALQLPVLLVVGMRLGCLNHA
                     LLTALAVQRRGLVLAGWIANELPPGMPLVQQNVEALAERLGCVPIVVAGPGERPVFHA
                     AQLAMLGLVA"
     gene            complement(4540..5472)
                     /gene="bioC"
                     /locus_tag="VFO53_12000"
     CDS             complement(4540..5472)
                     /gene="bioC"
                     /locus_tag="VFO53_12000"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HET8877044.1"
                     /translation="MTEPAYPAPDPRDVDPQAVRRAFAHAAATYDGAAALQHEIGMRM
                     AERLDYVKLAPSVILDAGCGTGDAVGDLGVRYPAARIVAFDIALPMVLAARARVRAGR
                     STFRRLLRPFAARTLSEPAFVCADINALPFDGVACDLVWSNLALQWVNDLPRAFAEFR
                     RVLKVGALLSFTTFGPDTLREVRAAFAGIDAKTHTNRFVDMHDIGDMLVHAGFADPVM
                     DMEYVTLTYDSPQALVAELKAIGASNRTRGRPRGLMGRTRWARAVARLEALRHDGRIP
                     ATFEVIYGHAWKVAPRTTPEGHAIVRVQRPGSRA"
     gene            complement(5465..6256)
                     /gene="bioH"
                     /locus_tag="VFO53_12005"
     CDS             complement(5465..6256)
                     /gene="bioH"
                     /locus_tag="VFO53_12005"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HET8877045.1"
                     /translation="MRDIECSAPGVHVESLGAGGPLVLLHGFAMNGGLFAPLLAELAR
                     THRVHVVDIPGHGRSPPLDPFELPRIVDAIDAAIDGADLTVLGWSLGGQIALAWALAR
                     PQRIRRLVLVATTPSFVERDGWRHAMSAQTLSRFGDELRASYRLTLQRFLALQVHGSE
                     EGRATLAALRQRLFERGEPSPEALAAALRALAQNDLRSALAHIAAPTLVIAGDRDALV
                     PLAATRELAAAMPNATHVTIEGAAHAPFLSHRRRFIDTVLSYVDD"
     gene            complement(6249..7412)
                     /gene="bioF"
                     /locus_tag="VFO53_12010"
     CDS             complement(6249..7412)
                     /gene="bioF"
                     /locus_tag="VFO53_12010"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HET8877046.1"
                     /translation="MLAELEAALERRRTDGLLRERRVVESAQGPRIRCGDRTLLAFAS
                     NDYLGLANAPELVDALCASARAWGAGAGASHLIVGHQAPHEALEQELAAFVAPGANAR
                     ALVFSSGYLANLAILSSLASRHDAIFADRLNHACLTDGARLSRATLTRYPHLDVGALR
                     DALGRVRARRRFIVTDAVFSMDGDIAPLADLLELAQTHDAWLVVDDAHGFGVLGGGRG
                     TLAHLGLCSERIVCMGTLGKAAGVAGAFVAAHPLVIETLVQTARPYIYTTASPAPLAE
                     TLRVALRVVRDADARRARLATLIGAFRAQAAGLPWKLLASSTAIQPLVVGDAATAVRV
                     AEALAARGILVPAIRPPTVPAGTARLRISLSSAHTLADVDALARALREVAADA"
BASE COUNT         1266 a         2509 c         2578 g         1087 t
ORIGIN      
        1 ctgtcacgat ctctcgtgcc cctcgacctc gaagaacgtg tacgacagcg tgatcgtcgg
       61 cacgtccttc ggcagcttcg ggtcgacgac gaacacgacc ggcatgtcgc gcttctcgtg
      121 cggcgcgagc gtctgctgcg aaaagcagaa gcagtcgagc ttcttgaagt agagcgccgc
      181 ctgccggggc ccgtagcttg gaatggcctg cccggtgatc ggccggtcgg tggtgttgac
      241 gacctcgaac atcgcctgcg tcaccgcgcc gggatggacg tcggttgcaa gctgcagcgc
      301 gcgaaactgc cacggaaggt tgcgcaggtt cgtgtcgaat tcgatgcgca ccgagcgcgt
      361 cgtgtcgacc tgggtgttcg tcacggtgtc ggccgagtcg atgttgcgca gccccgtcac
      421 ctcgcagatc ttctcgtaga acggcacgag cgcgtagccg aagccgaaca tcgcgacgac
      481 gacgacgccg agcttcgtca gcagcgtgcg attgctccgc gcgacctcat cacgggtggg
      541 gtccccccac gctcgcggct tcgccacttc gctgcccccg gagggggcgc aattcgcgtc
      601 ttgggacggc ccggcggcgc tcattggccc ccccacgctc gcggcttcgc gccggtcatt
      661 tgcgcaggtg ccgcccgatg gcgacgacca ggtagagcag gatggccccg ccgacgacgg
      721 cgatcgatac catctcgctc tcgatgaagc gcgaggcgat gatgccgaag aagaacagca
      781 gcgcgatcgc gagcaggacg agtcccgtcc gccggttggc ggcccgaagg cgcgcgtcgc
      841 gctgcggctc attggccatg gagtcgagcg tcgatggcgg catcgtgtcg atcgggcgcg
      901 cgccgctatt tgacgaccgg cggcgtctcg aacgagtgat acggcgccgg cgacggcagc
      961 gtccactcga gcgtcgtggc gccgtcccac ggtttggccg gcgccggcgc gccgccgcgg
     1021 atgcacttca cgaccgtggc gaggaacagg agctgcgaca gcccgaagcc gaacgcgccg
     1081 atcgtcgaca ccatgttcca gtcggtgaac tgctgcgcgt aatccggaac gcgccgcggc
     1141 atgccggcga gcccgaggaa gtgcatcggg aagaatgcga cgttgaagaa gatgaacgac
     1201 agccagaaat gccagtcggc gagcttctcg tcgtacatgt ggccggtcca cttcggcagc
     1261 cagtaatacg ttccgccgaa gaatgcgaac agcgtgcccg cgaccatcac gtagtggaag
     1321 tgcgcgacga cgtagtacgt gtcctgaaac tgcacgtcga ccggctggat ggcgaggatc
     1381 agcccggtga agccaccgat cacgaacagg aacaggaacc ccagcgcgaa gagcatcggc
     1441 gcctcgagcg tgatcgagcc cttccacatc gtcgcgagcc agttgaacac cttcacgccg
     1501 gtcggaatgg cgatcagcat cgtcgcgtac atgtagaaga ggatgctcgc cgccggcatg
     1561 ccgaccgtga acatgtggtg cgcccagacg aggaacgaga ggatcgcgat cgcggcgagc
     1621 gcgtacacca tcgacgcgta gccgaacagc ggcttgcgcg agaacgtcgg aacgatttcc
     1681 gagatgatgc cgaacgccgg caggatgatg atgtagacct cggggtggcc gaagaaccag
     1741 aatacgtgct ggaacagcac cgggtcgccg ccaccggccg cgttgaagaa cgacgtgccg
     1801 aaatgccggt cggtgagcag catcgtgacg gcgccggcga gcaccggcat cacggcgatc
     1861 agcaggtagg cggtgatgag ccacgcccag cagaacagcg gcagcttcat cagcgtcatg
     1921 ccgggtgcgc gcaggttcag gatcgtggtg atgatgttga tcgagcccat gatcgacgac
     1981 gcgcccatga tatggatcgc gaagatcgtc aggtccatgc ccatgcccga ctggatcgat
     2041 agcggcggat agagcgtcca gcccgtttcg gcggcgccgc cgggcacgaa cagcgacagg
     2101 atcagcatga tcgccgcggg aggcagaagc cagaagctcc agttgttcat tcgcgcgaac
     2161 gccatgtcgg gcgccccgat ctgcatcggg atcagccagt tggcgaagcc cacggcgccg
     2221 ggcatcagtg cgccgaagat catgatgagg ccgtgcagcg acgtcatctg gttgaagaac
     2281 tcgggcttga cgatctgcag tccgggctgg aagagctcgc tgcggatcgt cagcgccatg
     2341 attccgccga cgatgaacat cgtgaacgag aaccacaggt acatcgtgcc gatgtccttg
     2401 tggttggtcg tcgtgatcca gcgcatgatc cccttcgggt gatgcgcgtg ctcgccgtgc
     2461 ggaacgccgc cgacaaccag gtcgtgtggg atgctggaca ttcgaactcc tccggctact
     2521 tccgttcggc cttcacggcc gacggctgga cgacgttctc gctcgccttg ttgccgaagc
     2581 cgttgcgggt gtacgtgatc accgacgcga tgtcgacgtc ggaaagctgg ctccacgaag
     2641 gcatcttgcc cccggtgggc gccttctgca cgaccccgtt ggtctggccg ttcagcacga
     2701 cgtcgatctg gtgctgagca ggaccgagca ccaccttgtc gccgacgagg gccggcgcac
     2761 cgatcgccgc gttgccgctg ccgttcgcct ggtggcacgc cgcgcagttc gcgttgaaca
     2821 cctgctcgcc atgcgcgacg agctccttct cgtcccagac cttgttgggg ttctgcgtgg
     2881 cggcgccgag cttctgcttc tgctcggcca cccacttcgc atagtcgtcc ttcgacttca
     2941 cctcgacgac gaccggcatg aagccgtgct ccttgccgca cagttccgcg cactggccac
     3001 gatagacgcc gaccttgtca gcacgaaacc acgtgtcgcg cacgaatccg ggaatggcgt
     3061 cctgcttcac gccgaacgcc ggcacccacc aggaatggat cacgtcgttg gcggtgacca
     3121 gcacgcgcac cttggcgcct accggcacga cgagcgggtt gtcgacctcg agcaggtaat
     3181 gctcgccctt cggcgcgcgg ccctcgatct ggtcgagcgg cgtcgcgagc gtcgagtagt
     3241 agccgaagcc ttcctgcatg tagtcgtagt tccacttcca ctgatagccg gtgaccttga
     3301 tcgtcatgtc cgacgccgcg gtgtcgtgcg acgcgaggat cgtcttcgtc gccggccacg
     3361 ccatgaacag caggatcagg aacggaatga ccgtccagat cacctcgacc gcggtgttct
     3421 cgtggaactg caccgcctgg tgcccgaccg acttgcggtg ccggtacagc gaatagaaca
     3481 tcgtgccgaa cacgccgacg aagatcgcga cgcagatcca gaagatgagg tcgtgcagcg
     3541 tgatgatttc gcgcgccagc ggcgtggccg gcggctggaa ataccagtcg ggcgccgcga
     3601 gcgcggccgc cggcgcggcg agcaatgcgg cggttgcggc acggcgaagc caccgcgcga
     3661 gttcccttcg tttacacacc atgcattccc tctgtcatga cacggcctcg ccatgtccgg
     3721 cggcttcgaa tcgcacgcgg aaaaaacgcg cgtcggctga cgcgcgcttt tcaaaggatg
     3781 caaatgttag cacggaacgt tagagccgtc gtcgacacga tgcgtgcccg gcgcgcacgc
     3841 aggctcatgc gacgaggccg agcatcgcaa gttgcgcagc atggaaaacg gggcgctcgc
     3901 cgggaccggc gacgacgatc ggcacgcaac cgaggcgctc ggcgagcgct tcgacgttct
     3961 gctgcacgag cggcatgccg ggcgggagct cgttcgcgat ccagccagca agcacgagcc
     4021 cgcggcgttg cacggcgagt gccgtcagca gcgcgtgatt gaggcagccg aggcgcatcc
     4081 cgacgacgag cagcaccgga agctgcagcg cagcggcgat gtcgagcatg tcgcgcgtgc
     4141 cgtcgagcgg cacgagcgca ccgccggcac cctcgaccac cagcgcgtcg gcgcgctcgc
     4201 gcaatcgccc ggcggcgccg acgatcgcgg gcatcgcaat gtcgatccct gccgcggccg
     4261 cggcaagatg cggcgcaacc gcgtcggcga acgcgtacgg gttgcagtct gcaagcggca
     4321 cgtcgacgtt gccggcggcg cgcaatgcaa tcacgtcgcc gttgatgttc gccgcgggct
     4381 cgaagcccgc cgcgaccggt ttcatgccga cggcacgcac gccggcgcga gcgagcgcgt
     4441 gcagcagcgc ggttgcgatg cgcgttttgc cgacgcccgt gtcggtgccg gtgacgaaga
     4501 tcccgcgcga ggaggcggcg gtgcgattca cgaccgcgct cacgcccgcg acccggggcg
     4561 ctgcacgcgc acgatcgcat ggccttcggg tgtcgtgcgc ggcgcgactt tccacgcatg
     4621 gccgtagatc acctcgaacg tcgccgggat gcggccgtcg tgccgcagcg cttcgagcct
     4681 tgcaacggca cgcgcccagc gcgtgcgccc catcaggccc cgcggccgcc cgcgcgtgcg
     4741 gttcgacgca ccgatcgcct tcagctccgc gacgagcgcc tgcggactgt cgtaggtcag
     4801 cgtcacgtac tccatgtcca tgaccggatc ggcgaagccg gcgtgcacga gcatgtcgcc
     4861 gatatcgtgc atgtcgacga agcgattcgt atgcgtcttc gcgtcgatgc cggcgaatgc
     4921 ggcgcgcacc tcgcgcagcg tgtcggggcc gaacgtcgtg aacgacagca gcgcgccgac
     4981 cttcagcacg cggcggaact cggcgaatgc gcgcggcagg tcgttcaccc actgcagtgc
     5041 aaggttgctc cagacgaggt cgcaggcgac gccgtcgaac ggcagcgcgt tgatgtcggc
     5101 gcacacgaat gcgggctcgg acagggttcg cgccgcgaac ggccgcagca gccgccgaaa
     5161 ggtgcttcgc ccggcgcgca cgcgtgcacg cgcggcgagg accatcggca gcgcgatgtc
     5221 gaacgcgacg atgcgcgccg ccggatagcg aacgccgaga tcgccgaccg cgtcgccggt
     5281 gccgcagccg gcgtcgagga tcaccgacgg cgcgagcttc acgtagtcga gccgctcggc
     5341 catccgcatg ccgatttcgt gctgcaaggc cgccgccccg tcgtacgtcg cggccgcatg
     5401 cgcgaaggcg cgccgcaccg cctgcggatc gacgtcgcgt ggatcgggcg ccggataggc
     5461 gggctcagtc atcgacgtac gacagcaccg tgtcgatgaa gcgtcgccga tgcgacagga
     5521 aaggcgcatg cgccgcgccc tcgatcgtca cgtgcgtggc attcggcatt gcggcggcga
     5581 gctcacgcgt tgcagcgagc ggcaccagcg cgtcgcggtc gcccgcgatc acgagcgtgg
     5641 gtgccgcgat gtgcgccagc gccgatcgca gatcgttctg cgcgagggcg cgaagcgcgg
     5701 cggcgagcgc ctccggcgat ggctcgccgc gttcgaagag ccgctgccgc aacgcggcga
     5761 gggtggcccg cccttcctcg ctgccgtgca cctgcagcgc gaggaaccgc tgcagcgtga
     5821 ggcgatagga agcgcgaagc tcgtcgccga agcgcgacag cgtctgcgcg ctcatcgcgt
     5881 gccgccagcc gtcgcgctcg acgaacgacg gcgtcgtggc gacgagcacg agccgccgga
     5941 tccgctgcgg gcgcgcgagc gcccacgcaa gcgcaatctg cccgccgagc gaccagccca
     6001 gcaccgtgag atcggctccg tcgatcgccg cgtcgatcgc gtcgacgatg cgcggcagct
     6061 cgaacgggtc gagcggcggc gagcgcccgt ggccgggaat gtccacgaca tggacacggt
     6121 gcgtgcgcgc aagctccgcc aacagcggcg cgaagagtcc gccgttcatg gcgaagccgt
     6181 gcagcagcac gagcggaccg cccgcgccca gcgattcgac gtgcacgccg ggagcgctgc
     6241 attcgatgtc acgcatcggc cgcgacctcg cgcagcgcgc gggcgagtgc atcgacatcg
     6301 gccagcgtat gtgcggacga cagcgaaatc cgcagccggg cggtgcccgc gggcaccgtc
     6361 ggcggccgga tggcgggcac gagaatgccg cgcgcggcga gcgcttccgc gacgcgcacg
     6421 gctgtcgccg cgtcgccgac gacgagcggc tggatcgcgg tcgacgaggc aagaagcttc
     6481 cacggcagcc cggccgcctg cgcgcgaaac gcgccgatca gcgttgcgag ccgggcgcgc
     6541 cgggcgtcgg catcgcgaac gacgcgcagc gccacgcgca gtgtttcggc gagcggcgcc
     6601 ggcgatgcgg tcgtgtagat gtacggccgc gcggtctgca ccagcgtctc gatcacgagc
     6661 ggatgcgcgg cgacgaacgc gcccgccacg cccgccgcct tgccgagcgt gcccatgcag
     6721 acgatgcgct cggagcagag cccgagatgc gcgagcgttc cgcgtccgcc gcccagcacg
     6781 ccgaacccgt gcgcgtcgtc gacgacgagc cacgcatcgt gcgtctgcgc gagctcgagc
     6841 aggtcggcga gcggcgcgat gtcgccgtcc atgctgaaca ccgcgtcggt cacgatgaac
     6901 cggcgccggg cgcgcacgcg cccgagcgca tcgcgaagcg cgccaacgtc gagatgcggg
     6961 tagcgcgtga gggtcgcgcg cgacagccgt gcgccgtcgg tcaggcaggc gtgattcagc
     7021 cgatcggcga agatcgcatc gtggcggctt gcaagcgacg acagaatcgc gaggttcgcg
     7081 aggtagccgc tcgagaacac gagcgcgcgc gcattggcgc cgggcgcgac gaatgccgcg
     7141 agctcctgct cgagcgcctc atgcggcgcc tggtgcccga cgatcaggtg cgacgcgccg
     7201 gcgccagcgc cccacgcgcg tgccgaggcg catagcgcgt cgacgagctc cggcgcattc
     7261 gcgaggccga gataatcgtt gctcgcaaac gcgagaagcg tgcgatcgcc gcaccggatg
     7321 cgcggaccct gcgcgctttc gaccacgcgc cgctcgcgca ggagtccgtc ggtgcgacgc
     7381 cgttcgaggg ccgcttcgag ctcggcgagc attgcggcgt caaccgcgcg ctacgcctcc
//
LOCUS       DASSVX010000279        16814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1612368, whole genome shotgun sequence.
ACCESSION   DASSVX010000279 DASSVX010000000
VERSION     DASSVX010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 16814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16814
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612368"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFO53_12015"
     CDS             complement(<1..626)
                     /locus_tag="VFO53_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877047.1"
                     /translation="MLKRILPVIVAAFALASGPAVAQAKEKVVLLLNWYTYSEHAPFY
                     LGKERGYFDQEGIDLDIQEGRGSGVTVQAVAAGTAAFGYADVPTMMKAASKGAPVIAV
                     GVALQTSPMSVMGFADKNIRKPDDIKGKTVAVTPGDSMSQIWPLFLQKTGLKQSDFKT
                     VAGDAQTKLNAVINGQADLLLGYVMDQAIKLQDATHKPVYPIRFADYGV"
     gene            complement(670..1455)
                     /locus_tag="VFO53_12020"
     CDS             complement(670..1455)
                     /locus_tag="VFO53_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8877048.1"
                     /translation="MWRRVLYSSWARPIILIVLLIVLWQLAIVVFRIPPYLIPAPLAV
                     VKQLVTQWPELLQQASFTTYATLGGFALSIAFGIPMALLIAYSRTVESFVYPLLVFSQ
                     SVPKVAIAPLFVVWFGFGIIPKVIAAFMLGFFPIVVSTVVGFKSVDRDMIDLARSMKA
                     SKLQIFRRISLPQALPSIFAGLKVSVTLAVVGAVVGEFVGSNSGIGYVLQIANGNFDL
                     PLMFAALTILSLIGVLLFAAVDLAERFMIPWHASQRNEFSATA"
     gene            complement(1600..2268)
                     /locus_tag="VFO53_12025"
     CDS             complement(1600..2268)
                     /locus_tag="VFO53_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET8877049.1"
                     /translation="MDAVTDGKRARSPVHRQGTRDSGRTQAAILTAATREFARHGLGG
                     ARVDRIAAGARTNKRMLYYYYGSKEALFVAVLEDCYARIRRAEKALSLLDVDPVESVR
                     RLVEFTWNYYLRHPEFLTLLNSENLHGACHVRKSPNVREMNSPLIETLGEVLERGRGS
                     GVFRRDVDALQLYVTIAALSYFYLSNNQTLSAVFGRNLATPQARRERLQHMVDVVLGY
                     LQSG"
     gene            2442..2849
                     /locus_tag="VFO53_12030"
     CDS             2442..2849
                     /locus_tag="VFO53_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET8877050.1"
                     /translation="MGDLKVKVPDTEKITINVGCVDLGQIDLLVQEGFYSNRTDLIRT
                     AIRNQIAVHADVLRQTVARRTLVLGLLDYSRRDLETVRKSGRKLRIQALGLVRIADDV
                     SPALAQDTIESITVLGALHASAAVKAALSDRLH"
     gene            2867..4006
                     /locus_tag="VFO53_12035"
     CDS             2867..4006
                     /locus_tag="VFO53_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET8877051.1"
                     /translation="MIFDMNGTMNEAMQRMSTGDLTGATRLIQRRLSATSAPAAATQE
                     LATTRGNTIEGTWREVGGAPRADAPWPGQTRSGRDFRDEHEGAFREHRFTADAGELRY
                     KLFVPRGLADPAPPLIVMLHGCTQSPDDFARGTRMNALAQEHGYVVAYPAQSKRRNAT
                     RCWNWFRSGDQQRGRGEPALISGLARHLVNAHRLDARRVYVAGLSAGGAMAAVIADTY
                     PDVYAAIGVHSGLPVGAAHDLASAFAVMTRGTAQSADRGSRSGRRGVPAIVFHGDCDT
                     TVDACNGAAIVSQCAGTDGCASPAEAQMRESRESGRVPDGRSYTRTVYRGAEGALLAE
                     QWLVHGGGHAWFGGDPSGSYTDSSGPDASAEMLRFFSVCARGAPN"
     gene            complement(4038..5357)
                     /gene="glgC"
                     /locus_tag="VFO53_12040"
     CDS             complement(4038..5357)
                     /gene="glgC"
                     /locus_tag="VFO53_12040"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET8877052.1"
                     /translation="MADTHGVQFGYGPAKRDPQGAYLSQLARDTLAIVLAGGRGTRLG
                     PLTDWRTKPAVPFGGKFRIIDFSLSNCVNSGVRRIGICTQYKAQSLIRHVQRGWSFLD
                     GRFREFVELLPAQQRVTGDWYKGTADAVYQNLDILRRHAPEFVLVLAGDHVYKMDYAR
                     LLAEHVERAADVSVGCVEVPIALASQYGVMDLDAESRVVGFDEKPAHPRPAPGQPDVA
                     LASMGIYVFNASFLFNELIRDAKQTRSTHDFGRDVIPSLIADAKRVFAHRFAASCVNM
                     AGDRPYWRDVGTIDAYWEGNLDLTHVTPDLNLYDDDWPIWTYQEQLPPAKFVFETDER
                     RGSAFDSLVSGGCVISGSSVRRSLLFSNVRVHSYSDIQDAVILPDVEVHRHVMLRNCI
                     IDRYCALPAGLQVGVDPDEDRKRFHVTERGVTLVTPEMLGQHVHGLP"
     gene            5460..6944
                     /gene="glgA"
                     /locus_tag="VFO53_12045"
     CDS             5460..6944
                     /gene="glgA"
                     /locus_tag="VFO53_12045"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET8877053.1"
                     /translation="MSPLPPLRILYATSEYAPLVKTGGLADVAGALPAALRRLGLDVR
                     VLVPGYQAVIDALGRPQRTTRVHSIAPLPAVDLLEVRLPGDVPAWIIDCPALYRRAGG
                     PYLDAAGVDWDDNPLRFGLLSRIAALLGTAQSGVEWQAQLVHANDWQCALAPAFGALL
                     HDSTTPCIVTIHNLAYQGVFAQHWVTDLGLPAECFRVEGVEYYGRFSFLKAGLFYADA
                     ISTVSPTYAAEIQSAPAGMGLEGLLAGRRDVLTGILNGIDTTVWDPRNDVLIPLRYDA
                     TRLHRKAANRRALQMRFHLEPRADTLLLGAVSRLTDQKGIDLLLDAVDAIVRLPAQLV
                     IVGAGDKALEARLRVLADAHPGKAAGFVGFDESLAHLVEAGADAFVMPSRFEPCGMNQ
                     MYSQRYGTPPIVHATGGLVDSVVDCTPATMTRGTATGFTFDAPVVSSLLGAIERCVAA
                     FRQRPTWQALQRNGMVRDFGWDRAAKEYAALYAATQARVDRRSE"
     gene            complement(6970..7062)
                     /locus_tag="VFO53_12050"
                     /pseudo
     CDS             complement(6970..7062)
                     /locus_tag="VFO53_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052629.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
     gene            complement(7050..7571)
                     /locus_tag="VFO53_12055"
     CDS             complement(7050..7571)
                     /locus_tag="VFO53_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide isomerase"
                     /protein_id="HET8877054.1"
                     /translation="MPTHADLLTQLEKTDVVREGPAQSTHVLYVFFDANCLYCHLTWK
                     ALQPYETAGLQVRWVPVAYQQPSSVGRAAAIMQAPDRAAALRLNELRYDASKYDGGIE
                     PATTVSAAMVAQLQANTRLMQTIAPGTPVLVWKDGAGKVRVKVGVPRLSALPDITGLP
                     QQTNDDPELAQFR"
     gene            7873..9270
                     /locus_tag="VFO53_12060"
     CDS             7873..9270
                     /locus_tag="VFO53_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HET8877055.1"
                     /translation="MLRPDPTFYPSPRLAMEAPDEQYAYVALLRPDKTAPDALAVVDT
                     KPGSTTYGKAVHTVELTNVGDELHHFGWNACSSMLCPLNGHPFTERRYLIVPGMRSSR
                     LYIIDTKPGPTQAKIVKVIEPEELVRKTGYSRPHTVHCGPEGVYISTLGARGADGTDG
                     VPGIFLLDCDSFDILGRWEMERGDQRLSYDFWWNIPHDYMISSEWGLPPQFENGLVGE
                     DLLGNRYGHRLHFWDLRARRHVQTIDLGANHQMALEVRPAHEPTKDYGFLGVVVDTTN
                     LEGSIWTWWRENGQFKARKTAVVPPQPADPAKLPGLLKPFNAVPPLISDIDLSLDDKY
                     LYVACWGTGELRQYDVSDPMQPRLSGSVKVGGVVDHVAHPSGKAFGGGPQMVEISRDG
                     ERVYFTNSLYSSWDDQFYPDGVPGAMVMAKAKPGGGIELEREFCVEFVAGYRSHQVRL
                     EGGDCSTDSYCFPSA"
     gene            9280..9957
                     /locus_tag="VFO53_12065"
     CDS             9280..9957
                     /locus_tag="VFO53_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877056.1"
                     /translation="MQGLAQSLAQHPWAWGAIVALGAWHGLNPGMGWLFAVSSGLQAR
                     RAEGVFRALPPLALGHFVAMLAALLPFTLFMLYFERVGAIRAAAGGLLVIFGVYKLLS
                     RRHPRFLARIGPSHLVVWSFFIATAHGAGLMLVPVVLGLCSTNAPEAQRGHQALLELA
                     SGSLGMTLAAVSVHTMAMLVTGGTIAWVVYRYVGLRLLRSAWVNVDLLWAALLVVAGA
                     IALAVTL"
     gene            9974..11050
                     /gene="ligD"
                     /locus_tag="VFO53_12070"
     CDS             9974..11050
                     /gene="ligD"
                     /locus_tag="VFO53_12070"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006139852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET8877057.1"
                     /translation="MPATHAETVHVDGRDVIVTNPGKLLFPETGHTKLDVVRYYLSVA
                     EGALRGSGGRPNVLVRYPDGIDGEFFFQKRAPKDRPPWIDAVTISFPSGRTAEEIVPR
                     HAADLAWMANLGCLELHPHPVRATDLDHPDELRIDLDPVANVPWSQVREVARVVYHVL
                     ERHALQGFLKTSGKRGLHVLLRIDRRWDYGQVRRAALALARDVEQRVPALATTRWWKE
                     ERHGVFIDYNQNAKDRTVAGAYSIRPTPDARVSAPLDWSELDTADPADFTLRTMPTRF
                     ASIGDRHEAIDARAYSLDSLLELSAQHERDGQGDAPWPPQYAKAPGEPRRVQPSRARA
                     GTAANAPPAGDAPATTKRPPRKPS"
     gene            complement(11019..12098)
                     /locus_tag="VFO53_12075"
     CDS             complement(11019..12098)
                     /locus_tag="VFO53_12075"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET8877058.1"
                     /translation="MSFPIAPPIEPMLAQVAEQLPAGGEFLFEPKWDGFRAIVFRSDD
                     DLFIQSRDLKPLDRYFPELHDAMRRLLPGGCVVDGEIVVVTPHGLDFDALQLRLHPAA
                     SRIARLAKSSPASFVAFDVLAVDGRSVMTALLRDRRAMLEALLAHVEPPLHLTPMTRQ
                     PELAYDWLQQFEGAGLDGVVVKPVDIAYLPGKRAMFKVKHARTADCVVAGFRWYKDTR
                     DAVGSLLLGLYDERGVLHHVGVTSAFTMATRRSLLDILEPLRRNALDAHPWRDWANEQ
                     AHATTRMPGGQSRWSAGKDLSWEAVRIELVCEVKYDHLQGDRFRHATHFVRWRPDKPA
                     QACRYDQLETTPPYELTKVFGAAAS"
     gene            12277..13107
                     /locus_tag="VFO53_12080"
     CDS             12277..13107
                     /locus_tag="VFO53_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET8877059.1"
                     /translation="MSNVFGRALLALLLGSWTAIAAAAVAVPAAGASPRIDAIRKAGV
                     LRVGVLANAPWLVENTSGQGEAWSGPAWLLANDYAKRLGVRLQAIPVSHETKVPVLAS
                     NQVDISVTPLAETPERLKVVDFVIYSTTSVCMYGLASNPKFANAKSVDELNRPDITIA
                     YYTGGGEEGWVKERFPKAKLRGVAGSGISPVEEVMAKRADAVPINRVPWVAMGRKLTQ
                     LAVLPKANNCQDSKEKAAPVGMAIDKNQDAFLQWARAVEKEIEPQLRKDEMRVVETMK
                     "
     gene            13165..13830
                     /locus_tag="VFO53_12085"
     CDS             13165..13830
                     /locus_tag="VFO53_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET8877060.1"
                     /translation="MHLDYGVITTNWRFLADGVWITVLLAVASGAASLFVGFVLALLR
                     LRGPRWLRPAIVFYIDSMRAIPVLVVLVWTFFALPIVANVTMSPFTAGLIGLTVHLAA
                     YAAEIVRAGIESVRPGQTRAALALGMSHPQILRRVVLPQALVRMLPAFGSLLSVTIKD
                     TAIASVIAVPELMRRAETVAGQSFQPIEVYTFAMLVYFVLLYPVTRGVDRFYRRVAHL
                     GRS"
     gene            13830..14489
                     /locus_tag="VFO53_12090"
     CDS             13830..14489
                     /locus_tag="VFO53_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET8877061.1"
                     /translation="MTLDWSVVWQYRAALAAGTATTFLLTIATMAIALPCGIVVAGLR
                     LYGAAPLRAVATGYVELFRNLPLILVVYWAFYVLPILTGVGLSALATGLAALSLNVTA
                     YNAETFRAGINSIRRGQVEAALALGMTRTQALRRIVLPQALRRVLPVLASTWVSLFKD
                     TSLVSVIAVTELAYVSMQVRAQTFRVLEMLTAMAIIYWLLGYPQAKLVDWIQRRYAVT
                     E"
     gene            14486..15262
                     /locus_tag="VFO53_12095"
     CDS             14486..15262
                     /locus_tag="VFO53_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET8877062.1"
                     /translation="MSGRVSQREKATGSSVVRYLDVVKRYGSFTALAGVSLDVHAGEV
                     VCLIGPSGSGKSTLLRCTNGLETIDGGEIEFEGAKLGRDERTLRAMRQRMGMVFQNFE
                     LFPHLTAEQNVAEGPRTVLRTGLAEAKRRAAAMLTKVGLADKAASYPSALSGGQQQRV
                     AIARALAMEPDVMLFDEPTSALDPETIGEVLNVMKVLADEGMTMLVVTHEMSFARRVG
                     DWVAVFDAGRIVEEGPPEQIFERPATTRTRDFLSHLGWDA"
     gene            complement(15266..16162)
                     /locus_tag="VFO53_12100"
     CDS             complement(15266..16162)
                     /locus_tag="VFO53_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HET8877063.1"
                     /translation="MQQKLPWILSAGRFGHTMAAAPAIELQRTRFGPPFHASVEQRHK
                     KISREIRMSNKCKTCEVPGTVDRRGFLSASSALGALALAGGWGLSGVAHAVALTKAQR
                     DKLTPDDILALMKKGNKRFTSGRREDHNYLAQQRATASGQYPLAVLLSCIDSRAPAET
                     IMDLRIGDTFNSRVAGNIENPDILGSMEFACKIEGAKVVLVMGHTNCGAIKGAIDNAE
                     LGNLTGLLAKIKPAVDATKSAGERTSKNYAFVDAVGRKNVEMTVANIRKNSPVLAGME
                     SSGTIKIAGSMYDLKTGAVDFL"
     gene            16214..>16814
                     /locus_tag="VFO53_12105"
     CDS             16214..>16814
                     /locus_tag="VFO53_12105"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET8877064.1"
                     /translation="MKFGIFIYDGVEPIDLATYGVLSMARRVAPAIEIVTIAPAPGVV
                     NLSNGLRVIADHGIDDAPRCDVLVITGGPGWEAQSQSTATIAFLRAQPAATRIVSVCT
                     GAMILAATGMLDGHKATTKCQVRSPESSPLELMRQRHPAIDAQSASVVDCGALTTGGG
                     VSLCIDTMLHVIARELGAPVAKETARILEYSRAWDANRQQ"
BASE COUNT         2833 a         5537 c         5549 g         2895 t
ORIGIN      
        1 acgccgtaat cggcgaaacg gatcgggtac accggcttgt gcgtggcgtc ctgcagcttg
       61 atcgcctggt ccatcacgta gccgagcagc aggtcggcct ggccgttgat cacggcgttg
      121 agcttcgtct gcgcatcgcc ggcgaccgtc ttgaaatcgc tttgcttgag tccggtcttc
      181 tgcaggaaca gcggccagat ctgcgacatc gaatcgcccg gcgtgacggc caccgtcttg
      241 cccttgatgt cgtccggctt gcggatgttc ttgtcggcga agcccatcac cgacatcggc
      301 gacgtctgca gcgcaacgcc gacggcgatc accggtgcgc ccttcgacgc cgccttcatc
      361 atcgtgggca cgtcggcgta cccgaacgcg gccgtaccag ccgccacggc ctgcacggtg
      421 acgcccgagc cgcggccctc ctggatgtcg aggtcgatgc cttcctgatc gaaatagccg
      481 cgctccttgc cgagatagaa cggcgcatgc tcgctgtacg tgtaccagtt gagcagcagg
      541 acgaccttct ccttggcctg ggcgacggcg gggccggacg ccagcgcgaa cgcggcgacg
      601 atgaccggca ggattcgctt caacatggat ctcctccttg ttacgacgac gttgcgaagc
      661 cgctgcccgt caggcagtgg cgctgaattc gttgcgctgc gacgcgtgcc acgggatcat
      721 gaagcgctcg gcaagatcga ccgccgcgaa cagcaatacg ccgatgagcg acaggatggt
      781 gagtgccgcg aacatgagcg ggagatcgaa attgccgttc gcgatctgca gcacgtagcc
      841 gatcccggag ttcgagccga cgaattcgcc gacgaccgcg cccaccacgg ccagcgtgac
      901 cgacaccttg agcccggcga agatcgacgg caacgcctgc ggcaggctga tacgccggaa
      961 gatctgcagc ttgctcgcct tcatcgagcg ggcaagatcg atcatgtcgc ggtcgaccga
     1021 cttgaagccc acgacggtcg acacgacgat cgggaaaaag ccgagcatga acgcggcgat
     1081 caccttcggg atgatgccga agccgaacca cacgacgaaa agcggcgcga tggcgacctt
     1141 cggtacgctt tgcgagaaga cgagcagcgg atagacgaag ctttccaccg tgcgcgaata
     1201 ggcgatcagc aacgccatcg ggatgccgaa ggcaatcgac agcgcgaatc cgccaagcgt
     1261 cgcatacgtc gtgaaggatg cctgctgcag gagctcgggc cactgcgtga ccagttgctt
     1321 cacgaccgcg agcggcgcgg gaatgaggta gggcgggatg cgaaacacga cgatcgcaag
     1381 ctgccacagc acgatcaaca gcacgatgag gatgatcggc cgcgcccacg acgagtaaag
     1441 cacgcgccgc cacatcgagg tttgcatggt ttctccgcga agcttcggag gtgcaggaac
     1501 ggccgtcgag cgggcgcgcg aggcgccgca tcaagtaacc agccggttaa ttatccgcgc
     1561 cggctgcgcc gggtgtcaaa tgtcgcgccg ccgcgttcgt cagcccgact gcaggtagcc
     1621 aagcaccacg tcgaccatgt gctgcagccg ctcccttctc gcctgcggcg ttgccagatt
     1681 gcggccgaac acggccgaca gcgtctggtt gttcgacagg tagaaatacg aaagtgcggc
     1741 gatcgtcacg tacagctgca gcgcatcgac atcgcgacgg aacaccccgc tgccacgccc
     1801 gcgctcgagc acctcgccga gcgtttcgat cagcggtgaa ttcatttcac gcacgttcgg
     1861 cgacttgcgc acgtggcacg cgccgtgcag gttctcgctg ttgagcaacg tcaggaattc
     1921 ggggtggcgc aggtagtaat tccaggtgaa ctcgacgaga cgccgcacgc tttcgaccgg
     1981 atcgacatcg agcagcgaca gcgccttttc ggcccggcga atgcgcgcat aacagtcctc
     2041 gagcaccgcg acgaacagcg cttccttact gccgtagtag tagtagagca tccgcttgtt
     2101 cgtgcgtgcg ccggcggcga tgcggtcgac acgcgcgccg ccgaggccgt ggcgtgcgaa
     2161 ttcgcgcgtg gcggccgtca ggatggccgc ctgcgtacgc ccgctgtcgc gcgtgccctg
     2221 ccggtgaacg ggtgaccttg cgcgtttgcc gtccgtgacc gcgtccattg ccgtatgtta
     2281 accagacggt taacgctttt ccagcgcggc gcaccgctcc gcgagaaggg aagtataggt
     2341 cgatcacgag ccggtgccgg gcggcggttg ttcggcttta ccatctgtcg ggatggtgtt
     2401 aggatggtct acggatggtt tgaatacgcg aaggaatcga catgggcgat ttgaaggtga
     2461 aggtccccga caccgaaaag atcacgatca acgtcgggtg cgtggacctc ggccagatcg
     2521 atctgctcgt gcaggaaggg ttctattcga accgcaccga tctcatccgg acggcgattc
     2581 gaaaccagat cgccgtgcac gccgacgtgc tgcggcagac cgtcgcgcgg cgcacgctcg
     2641 tgctcggtct gctggattac tcgaggcgcg acctcgaaac cgttcgcaaa tcaggcagga
     2701 agctgcgcat ccaggcgctc gggctcgtgc gcattgccga tgacgtgtcg ccggcgcttg
     2761 cgcaggacac catcgaatcg ataaccgtgc tgggtgcact gcacgccagc gccgcggtca
     2821 aggccgcctt gagcgatcgg cttcactgaa gaatggaacc actacgatga ttttcgacat
     2881 gaacggcacg atgaacgaag cgatgcaacg catgagcacg ggcgacctga ccggcgccac
     2941 tcgcctcatc cagcgccggt tgtccgcaac ctctgctccg gccgccgcaa cgcaggagct
     3001 tgcaacgacg cgcgggaaca ccatcgaagg gacgtggcgc gaggtcggcg gcgcgccgcg
     3061 cgcggatgcg ccatggccgg ggcaaacgcg atccgggcga gacttccgcg acgagcatga
     3121 gggcgcattt cgcgagcacc gcttcaccgc tgacgcgggt gagctgcgtt acaagctgtt
     3181 cgttccgcgc ggtctcgccg atccggcccc gcccctcatc gtcatgctgc acggctgcac
     3241 gcaatcgccc gacgattttg cgcggggcac gcggatgaac gcgctcgcgc aggagcatgg
     3301 ctacgtcgtc gcctatccgg ctcaatcgaa gcgcaggaat gcgacccgat gctggaactg
     3361 gtttcgcagc ggcgatcagc agcgcggtcg tggagaaccg gcgctgatca gcgggctcgc
     3421 gcgccacctg gtgaatgcgc accggctcga cgcgcgtcgc gtctacgtgg cgggactgtc
     3481 ggccggaggg gcgatggcgg ccgtgatcgc ggacacctat cccgatgtgt acgctgcaat
     3541 tggcgtgcat tccgggctgc cggtcggtgc ggcgcacgat ctcgcctctg cattcgccgt
     3601 catgacacgg gggactgcac agagcgccga tcgcggttcg cgctccggca ggcgcggggt
     3661 accggccatc gtctttcacg gcgattgcga caccacggtc gatgcgtgca acggtgcggc
     3721 tatcgtgtcg caatgcgcag gcaccgatgg ttgcgcatcg cccgccgaag cgcaaatgcg
     3781 cgagtcccgc gagagcgggc gcgtgcctga cggccgttcg tacacgcgaa cggtctaccg
     3841 aggcgccgag ggcgccctgc tggccgagca atggctcgtg cacggcggag ggcacgcgtg
     3901 gttcggcggc gacccgagcg gctcgtacac cgattcgtcg ggtcccgacg catcggctga
     3961 gatgttgcgc tttttctccg tatgcgcgcg cggcgcaccg aactgaagga acgggctaac
     4021 gggttcgatg ctggtgatca cggcaggccg tgcacgtgct gtccgagcat ttccggcgtc
     4081 accagcgtga cgccgcgctc ggtgacatgg aagcgcttgc ggtcctcgtc cggatcgacg
     4141 ccaacttgca gcccggccgg cagcgcgcag taccggtcga tgatgcagtt cctgagcatg
     4201 acgtgccggt gcacctccac atcgggaagg atcaccgcgt cctggatatc ggagtagctg
     4261 tgcacgcgca cgttcgaaaa cagcagcgac cggcgcaccg acgagccgct gatgacgcaa
     4321 ccgccggaga cgagcgaatc gaatgccgag cccctgcgct cgtcggtctc gaaaacgaac
     4381 ttcgccggcg gcaactgctc ctggtaggtc cagatgggcc agtcgtcgtc gtagagattg
     4441 aggtccggcg tgacgtgcgt gaggtcgaga ttgccttccc agtacgcgtc gatcgtcccc
     4501 acgtcacgcc agtacggccg gtcgccggcc atgttgacgc aggacgcggc gaaacggtgc
     4561 gcgaacacgc gctttgcgtc ggcgatgagg gacggaatga cgtcccttcc gaaatcgtgg
     4621 gtcgatcgtg tctgctttgc gtcgcggatc agctcgttga acaggaacga cgcattgaac
     4681 acgtagatgc ccatgctcgc gagcgcgacg tcgggctgtc ccggtgccgg gcgcgggtgc
     4741 gcaggctttt cgtcgaagcc caccacgcgc gactcggcgt cgagatccat cacgccgtac
     4801 tggctggcca gagcgatcgg cacctcgacg cagccgaccg atacgtccgc cgcacgctcg
     4861 acgtgctcgg ccagcagtcg cgcgtagtcc atcttgtaga cgtgatcgcc cgcgaggacg
     4921 agcacgaatt cgggtgcatg gcggcgcagg atatcgaggt tctggtacac ggcatccgcg
     4981 gtgcccttgt accagtcgcc cgtcacgcgc tgctgcgcgg gcagcagctc gacaaactcg
     5041 cgaaaacgcc catcgaggaa gctccagccg cgctgcacgt gacgaatgag gctctgcgcc
     5101 ttgtactgcg tgcagatgcc gatgcggcga acaccggagt tcacgcagtt cgacagcgaa
     5161 aagtcgatga tgcggaactt gccgccgaat ggcacggcgg gcttcgtgcg ccagtcggtc
     5221 agcggcccga ggcgcgtgcc gcgccccccg gcgaggacga tggcgagcgt gtcgcgcgca
     5281 agctgcgaca gatacgcgcc ttgcggatcg cgcttggcag gcccgtaacc gaattgcacg
     5341 ccgtgagtgt ctgccatggt cgtccttcgc ctgccggtcg cgttgccggt gctgctatag
     5401 tcgatccgcg cgttcgcgca ccaccgaacg gcgctcgcat gtcgtgatcg aacggttcga
     5461 tgtcgccgct gccgccgctt cgcatcctct acgccacgtc ggaatatgcg ccgctcgtca
     5521 agacgggcgg cctggccgac gttgcgggcg cgctgcccgc cgccctgcgt cgtctgggcc
     5581 tcgacgtgcg cgtcctggtg ccggggtacc aggccgtgat cgatgcgctc gggcgcccgc
     5641 aacgcacgac ccgcgtacat agcatagccc cacttcccgc tgtcgatctg ctcgaagtcc
     5701 gattgccggg cgacgtaccc gcgtggatca tcgactgtcc ggcgctgtac cggcgcgcag
     5761 gcggtccgta cctcgatgcc gccggcgtcg actgggacga caatccgctg cgcttcgggc
     5821 tgctgtcgcg catcgcggcg ctgctcggca cggcgcaaag cggcgtcgag tggcaagcgc
     5881 aactcgtcca cgccaatgac tggcagtgcg cgctcgcgcc cgcgtttggc gcgctgctcc
     5941 acgattcgac gacgccttgc atcgtcacga tccacaacct cgcgtaccag ggcgtttttg
     6001 cgcaacactg ggtgacggat ctcggcctgc cggcggaatg ctttcgcgtg gagggcgtcg
     6061 aatactacgg ccgcttctcg ttcctcaagg ccgggctttt ctatgcggat gccatcagca
     6121 cggtgagccc gacgtacgcg gccgaaatcc agagcgcacc ggcggggatg gggctcgagg
     6181 gcctgctcgc cggacgccgt gacgttctga ccggcatcct gaacggcatc gacaccacgg
     6241 tctgggaccc gcgaaacgac gtgctgatcc cgctgcgcta cgatgccacg cggctgcacc
     6301 gcaaggccgc caaccggcgc gcattgcaga tgcgcttcca tctcgagcca cgcgcggata
     6361 cgctgctgct cggggccgtg tcccggctca cggatcagaa aggcatcgat ctcctgctcg
     6421 atgccgtcga tgccatcgtt cgattgcccg cgcagctcgt gattgtcggc gccggcgaca
     6481 aggcgctcga agcgcggctg cgcgtgcttg ccgacgcgca ccccggcaag gctgccggct
     6541 tcgtcgggtt cgacgaatcg cttgcgcacc tcgtggaagc gggcgccgac gccttcgtga
     6601 tgccgtcgcg cttcgagccc tgcggcatga accagatgta cagccagcgt tacggaacgc
     6661 cgccgatcgt gcatgcaacg ggcggtctcg tcgactcggt cgtcgactgc acgcccgcaa
     6721 cgatgacgcg cggcacggcg accggattca cgttcgacgc gcccgttgtt tcatcgctgc
     6781 tcggcgcaat cgaacgctgc gtggcggcat tccggcagcg cccgacgtgg caggcgctgc
     6841 agcgaaacgg catggtgcgc gacttcgggt gggatcgcgc ggcgaaggaa tatgccgcgc
     6901 tctatgccgc gacgcaagcc cgagtcgatc gccgtagcga atgagcccgc cctcctgcgc
     6961 gagcccggct cacgcgtcga caagcacctc gaagccgccg tagatcagcc gcctgccgtc
     7021 gaacggcatc gcgttctcgt ccggttgaat caccggaatt gcgcgagctc gggatcgtcg
     7081 ttcgtctgct gcggcagccc ggtgatgtcc ggcagcgccg aaagccgggg tacgccaacc
     7141 ttgacgcgca ccttccctgc tccgtccttc cagaccagca cgggggtgcc cggtgcgatc
     7201 gtttgcatca accgcgtgtt cgcttgcagt tgcgcgacca ttgccgcgga aaccgtcgtc
     7261 gccggctcga tcccgccgtc gtatttcgac gcgtcgtagc gaagctcgtt caatcgcaac
     7321 gcggccgcac ggtcgggcgc ctgcatgatg gccgcggcgc ggccgacgga gctcggctgc
     7381 tgatacgcga ccggcaccca gcgcacctgc aggccggccg tctcgtacgg ctgcagcgcc
     7441 ttccaggtca ggtggcaata gaggcaattg gcgtcgaaga aaacgtagag cacgtgcgtg
     7501 gactgcgcgg ggccttcacg aacgacatcc gttttctcga gctgcgtgag caggtccgca
     7561 tgcgtgggca cgtggccggc gcgatcgccg gtcgcacccg cggcatgacc gaaaggaaaa
     7621 aaggcgaagg cgacgacaag tatccgcaat gaccggttca tctgttttcc cgcagcgata
     7681 caggtcaccg ataatgtcac gctcgtcgct tcgtcggtgg caaagtcgca acgaccgtcg
     7741 gcaaaccgac tgccacgtgc gtgcttcgca ctcaggtagc gcacgcactt gccgtgtgcg
     7801 cgccggaggt tcatcctgat gcggtaggtc gcgcgccctc attggtcccg gaggaggttt
     7861 gccatgcccg acatgctgcg tcccgatccc acgttctacc cgtccccgcg ccttgcgatg
     7921 gaagcccccg acgagcagta cgcctacgtc gcgctgttgc gaccggacaa gaccgcgccc
     7981 gatgcgctgg cggtggtcga caccaagcca ggttcgacca cgtacggcaa agccgtgcac
     8041 accgtcgagc tgacgaacgt cggcgacgag ctgcatcact tcggctggaa tgcgtgcagc
     8101 tcgatgttgt gtccgctgaa cgggcacccg ttcaccgagc gtcgctacct gatcgtgccg
     8161 ggaatgcgct cgtcgcggct gtacatcatc gataccaaac cgggtcccac gcaggcgaag
     8221 atcgtcaagg tgatcgagcc ggaggagctc gtcaggaaga ccggctattc gcgtccgcac
     8281 acggtgcact gcggccccga aggggtctac atcagcacgc tgggcgccag gggcgccgac
     8341 ggcaccgacg gcgtgccggg catcttcctg ctcgactgcg acagcttcga catcctcggg
     8401 cgctgggaga tggagcgcgg cgatcagcgc ttgagctacg acttctggtg gaacatcccg
     8461 cacgattaca tgatctcgag cgagtggggc ctcccgccgc agttcgagaa cggcctcgtc
     8521 ggcgaggatc tgctcggcaa tcggtacggg caccgtctgc acttctggga ccttcgcgca
     8581 cggcggcacg tccagaccat cgatcttggc gcaaatcacc agatggcgct cgaggtgagg
     8641 cccgcacacg agccgacgaa ggactacggc ttcctcggcg tcgtcgtgga cacgacgaac
     8701 ctggagggat cgatctggac ctggtggcgt gaaaatggtc agttcaaggc caggaagacg
     8761 gcggtcgtgc cgccgcaacc cgccgatccg gcgaagcttc ccggactgct caaaccgttc
     8821 aacgcggtgc cgccgctgat cagcgacatc gatctgtcgc tcgacgacaa gtacctgtac
     8881 gtcgcgtgct ggggaaccgg tgagcttcgc cagtacgacg tgtccgatcc gatgcaaccg
     8941 aggctgtcgg gttcggtgaa ggtcggcggc gtcgtcgacc acgtcgcaca tccgagcggg
     9001 aaggcattcg gcggcggtcc gcaaatggtc gagatcagtc gcgacggcga gcgtgtgtac
     9061 ttcaccaact cgctgtacag cagttgggac gaccagttct atccggacgg cgtgccgggg
     9121 gcgatggtga tggcgaaggc gaagcccggc ggcggcatcg agctcgagcg cgaattctgc
     9181 gtcgagttcg tggccgggta tcggtcgcac caggtgcgcc tggagggcgg cgactgctcg
     9241 accgattcgt actgctttcc atcggcgtga cgggaagccg tgcaggggct cgcgcagagc
     9301 cttgcccagc atccgtgggc atggggcgca atcgtcgcgc tcggcgcctg gcacggcctg
     9361 aacccgggca tgggctggct cttcgccgtt tccagcggcc tgcaggcgcg gcgtgcggag
     9421 ggcgtcttcc gcgctctccc gccgcttgcg ttgggtcact tcgtggcgat gctggcggca
     9481 ctgctgccgt tcacgctgtt catgctgtat ttcgaacgcg tcggcgcaat ccgcgcagca
     9541 gcgggcggac tgctggtgat cttcggcgtc tataaacttc tctcgcgacg tcatccccgc
     9601 tttctggccc gcatcgggcc gtcgcacctg gtcgtctggt cgttcttcat cgcgaccgcg
     9661 catggtgcgg gactgatgct cgttccggtg gtccttggtc tttgcagcac caatgcaccc
     9721 gaggcgcagc gcggacacca ggcgctgctc gaactcgcga gcggcagcct cggcatgacg
     9781 ctcgccgccg tgagcgtgca cacaatggcg atgctggtga ccggcggcac gatcgcctgg
     9841 gtcgtttacc ggtacgtcgg tctgcgcctg ctgcgcagcg cgtgggtgaa cgtggacctg
     9901 ctgtgggcgg cgttgctcgt cgtcgccggc gcaatcgcgc tcgccgtaac cctatgagtt
     9961 agccggcgcc tccatgcccg ccacccatgc cgaaaccgtt cacgtcgacg gacgcgacgt
    10021 gatcgtcacc aaccccggca agcttctgtt tccggaaacc ggccatacga agctcgacgt
    10081 cgttcgctac tatctcagcg tcgccgaagg cgcgcttcgc ggttcgggtg gccggcccaa
    10141 cgtgctggtt cgctatcccg atggcatcga cggcgagttc ttcttccaga agcgagcgcc
    10201 aaaggatcga ccgccgtgga tcgacgccgt caccatcagc tttccgtcgg gccgcaccgc
    10261 cgaggaaatc gtgccgcggc acgccgccga tctcgcgtgg atggcgaacc tcggctgcct
    10321 cgagctgcac ccgcatccgg tccgcgcaac cgatctcgac caccccgatg agctgcgcat
    10381 cgatctcgac cccgtcgcca acgtgccctg gtcgcaggta cgcgaggtgg cgcgggtcgt
    10441 gtaccacgtc ctcgagcgcc acgcactgca gggatttctg aagacgtcgg gcaagcgcgg
    10501 gctgcatgtg ctcctgcgca tcgatcgccg ctgggactat ggccaggttc gccgcgccgc
    10561 gctggcgctc gcgcgcgacg tcgagcagcg cgtgccggcg ctcgcgacga ccagatggtg
    10621 gaaggaggag cggcacggcg tgttcatcga ttacaaccag aacgcgaagg atcgcacggt
    10681 ggccggcgcg tattcgatcc ggccgacgcc cgacgcgcgc gtgtcggcgc cgctcgactg
    10741 gagcgagctc gacacggcag accccgccga cttcacgctt cgcacgatgc cgacgcgttt
    10801 cgcgagcatc ggcgaccggc acgaagcgat cgacgcgcgc gcatactcgc ttgacagcct
    10861 gctcgagctt tccgcgcaac acgagcgcga cgggcaaggc gacgcgccgt ggccgcccca
    10921 atacgcgaag gcgcccggcg aaccgcggcg tgtgcagccg tcacgcgctc gggcgggcac
    10981 cgccgcgaac gcgccgcctg ccggcgacgc gcccgccact acgaagcggc cgccccgaaa
    11041 accttcgtga gctcgtaagg cggcgtcgtc tcgagctgat cgtagcggca cgcctgcgct
    11101 ggcttgtcgg ggcgccagcg gacgaagtgc gttgcatggc gaaaccggtc gccctgcaag
    11161 tgatcgtatt tcacctcgca cacgagctcg atgcgcacgg cctcccacga caggtccttg
    11221 ccggcggacc agcggctttg cccgcccggc atgcgagtcg tcgcgtgcgc ctgctcgttc
    11281 gcccagtcgc gccacggatg cgcgtcgagc gcgttgcggc gcagcggctc gaggatatcg
    11341 agcagactgc gccgcgttgc catcgtgaac gcggaggtga cgccaacgtg gtgcagcacg
    11401 ccgcgctcgt cgtacaggcc gagcagcagc gaaccgacgg cgtcgcgcgt gtccttgtac
    11461 cagcggaatc cggccacgac acaatcggcg gtgcgggcgt gcttcacctt gaacatcgcg
    11521 cgcttgccag gcagataggc gatgtcgacc ggcttcacga ccacaccgtc gaggcccgcg
    11581 ccttcgaact gctgcagcca gtcgtacgcg agctccggct gccgcgtcat cggcgtcaga
    11641 tggagcggag gttccacgtg cgcgagcagc gcttcgagca ttgcacgccg gtcgcgcagc
    11701 aaggccgtca tcacgctgcg gccgtcgacc gccagcacat cgaacgcgac gaacgacgcg
    11761 ggggacgact tcgcgagcct ggcgattctc gacgcggccg gatgcaggcg aagctgcaat
    11821 gcgtcgaaat caagcccgtg gggcgtgacg acgacgattt cgccgtcgac gacacagccg
    11881 ccgggcagaa gcctgcgcat cgcgtcgtgc agctccggga agtagcgatc caaaggcttg
    11941 aggtcgcggc tctggatgaa caagtcgtcg tcgcttctga agacgatcgc gcgaaatccg
    12001 tcccatttgg gctcgaagag gaattcgccg cctgccggca gctgctcggc aacctgggcc
    12061 agcatcggct cgatcggcgg ggcgatcgga aatgacatgg cgcgtgacag cgtccggtcg
    12121 tgaacagggg acacgtcatt gtgctccagc cgcaacgtgc gccgtccgga cccgcgcgcg
    12181 gcgcggtttg acagccgcac gctgctcgcc aatcattgcc ggtcgccggc tgccatcccg
    12241 gcctgcctgc caccgctttc caacggagaa ttgctcatgt cgaacgtttt cggtcgcgcc
    12301 ctcctggcgc tgctgctcgg ttcatggacc gcgattgctg ctgccgcggt cgccgttccc
    12361 gcggccggcg cgagtccgcg catcgacgcg atcaggaagg ccggcgtgct gcgcgtcggc
    12421 gtgcttgcga atgcaccctg gctcgtcgag aacacgagcg gacagggcga agcgtggagc
    12481 gggcccgcgt ggctgctcgc caacgactat gcgaagcgcc tcggggtgag gctgcaggcg
    12541 attccggtga gccacgagac gaaggttccc gtcctcgcgt ccaaccaggt cgacatctcg
    12601 gtcacgccgc tcgccgagac gcccgagcgg ctcaaggtcg tcgacttcgt catctattcg
    12661 accacgagtg tgtgcatgta cggcctcgcg tcgaatccga aatttgcgaa cgcgaaatcg
    12721 gtcgacgagc tcaaccggcc cgacatcacg atcgcctatt acaccggcgg cggcgaagag
    12781 ggctgggtga aggaacgctt ccccaaggcg aagctgcgcg gcgtcgcggg ctcgggcatt
    12841 tcgccggtcg aggaagtgat ggcgaagcgc gccgatgcgg tgccgatcaa tcgcgtgccg
    12901 tgggtggcga tgggccgcaa gcttacgcag ctcgcggtgc tgccgaaggc gaacaactgc
    12961 caggacagca aggaaaaggc ggcgccggtc ggcatggcga tcgacaagaa ccaggacgcg
    13021 ttcctgcaat gggcgcgcgc ggtggagaaa gagatcgagc cgcagcttcg caaggatgaa
    13081 atgcgtgtcg tcgaaacgat gaagtaggcg gcggcgcgtc gcgcgacgca ttgctccgcc
    13141 acccgcactg gggccctcga ccgcgtgcac ctcgactacg gcgtcatcac caccaactgg
    13201 cgcttcctgg ccgacggcgt atggatcacc gtgctgctcg cagtcgcatc gggcgcggcc
    13261 agcctcttcg tgggcttcgt cctcgcgctg ctgcgcttgc gcggcccgcg ctggctgcgc
    13321 ccggcgatcg tgttctacat cgactcgatg cgcgcgatcc cggtgctcgt cgtgctcgta
    13381 tggacgtttt ttgcgctgcc gatcgttgcc aacgtgacga tgtcgccgtt cacggccggc
    13441 ctgatcggct tgacggtgca tctcgcggcg tatgcggccg aaatcgtgcg cgccggcatc
    13501 gagtcggtcc gccccggcca gacgcgcgca gcgctcgccc tcggcatgtc gcatccacag
    13561 atcctgcgcc gtgtcgtgct gccgcaagca ctcgtgcgca tgcttcccgc attcgggtcg
    13621 ttgctgtcgg tcaccatcaa ggacaccgcc attgcgtcgg tcatcgccgt gcccgagctg
    13681 atgcgccgag ccgagaccgt cgccgggcaa agcttccagc cgatcgaggt gtacacgttc
    13741 gcgatgctcg tctacttcgt gctgctctat ccggtcacgc gcggcgtcga tcgcttctac
    13801 cggcgcgtcg cacatctcgg gcgctcgtga tgacgctcga ctggtcggtc gtgtggcagt
    13861 accgcgccgc cctggctgcc ggcactgcga cgacgttcct gctcaccatt gcaacgatgg
    13921 cgatcgccct tccctgcggc atcgtcgtcg ccggactgcg tctgtatggc gcggcacccc
    13981 tgcgcgcggt tgccacgggg tacgtcgagc tgtttcgcaa tctgccgctg atcctcgtcg
    14041 tgtactgggc gttctacgtg ctgcccatac tgacgggggt cggcttgtcg gcgctggcga
    14101 cgggcctcgc cgcgctgtcg ctcaacgtga ccgcgtacaa cgcggagacg ttccgggccg
    14161 gcatcaactc gatccgtcgc gggcaggtgg aagccgcact cgcgctcggg atgacgcgca
    14221 cgcaggcgct gcgccgcatc gtcctgccgc aggcactgcg acgcgtgctg ccggtgcttg
    14281 ccagcacgtg ggtgtcgctg ttcaaggata cgtcgctcgt gtcggtgatt gccgtcacgg
    14341 agctcgcgta cgtatccatg caggtgcgag cgcagacctt tcgcgtgctc gagatgctga
    14401 cggcgatggc catcatctac tggttgctcg gttacccgca ggcgaagctc gtcgactgga
    14461 tccagcgccg ctacgcggtg accgaatgag cggccgcgtt tcgcaacggg aaaaagcgac
    14521 gggatcgagc gtcgtgcgtt acctcgacgt cgtgaagcgc tacggcagct tcacggcact
    14581 cgccggcgta tcgctcgacg tgcacgccgg tgaagtggtg tgcctgatcg ggccctccgg
    14641 gtccggcaag tcgacgcttc tgcgctgcac gaacgggttg gagacgatcg acggcggcga
    14701 gatcgagttc gaaggtgcga agctcggccg cgacgagcgc acgttgcgtg cgatgcggca
    14761 gcgcatgggc atggtgttcc agaatttcga gctctttccc cacctcaccg ccgagcagaa
    14821 cgtggccgag ggcccgcgca cggtattgcg caccggactt gccgaggcga agcgccgcgc
    14881 ggcggccatg ctcacgaagg tgggactggc cgacaaggcg gcgagctatc cgtcggcgct
    14941 gtccggcggc cagcaacagc gggtcgcgat cgcccgcgcg ctcgcgatgg agcccgacgt
    15001 catgctgttc gacgagccga catcggcgct cgatccagaa acgatcggcg aagttttgaa
    15061 cgtgatgaag gtgctggcgg acgagggcat gacaatgctc gtcgtcacgc acgaaatgtc
    15121 attcgcacgc cgcgtcggcg actgggtggc cgtgttcgac gccggacgca tcgtggagga
    15181 aggaccgccc gaacaaatct tcgagcggcc cgccacgacc cgcacccgcg acttcctgag
    15241 ccatctcggg tgggacgcgt aagctctaca gaaaatcgac cgcgccggtt ttcaggtcgt
    15301 acatggagcc ggcgatcttg atggtgccgc tcgattccat gccggcgagc accggactgt
    15361 ttttccggat gttggcaacc gtcatttcca cgttcttgcg ccctacggca tcgacgaagg
    15421 catagttctt cgacgttctt tccccggcgg atttcgtggc gtcgacggcg ggcttgattt
    15481 tcgccagcag gccggtgagg ttgccaagct cggcattgtc gatcgcaccc ttgatcgcgc
    15541 cgcaattcgt atggcccatg acgaggacca ccttggcgcc ctcgatcttg cacgcgaatt
    15601 ccatgctgcc gaggatgtcc ggattttcga tgttacccgc cacgcggctg ttgaaggtgt
    15661 cgccgatgcg cagatccatg atcgtttcgg cgggcgcgcg ggagtcgatg cagctcagca
    15721 gcacggccag cggatattgt ccggacgcag tggcgcgttg ctgtgcgagg taattgtggt
    15781 cttcacgccg cccgctcgtg aagcgcttgt tgcccttctt catcagcgca aggatatcgt
    15841 cgggagtcag cttgtcgcgc tgcgccttcg tcagcgcaac ggcatgcgcc actccactga
    15901 gcccccaccc gccggcgagc gccaatgcac caagcgccga tgaagcgctc aggaaaccgc
    15961 gacgatcgac cgtgcccgga acttcacacg ttttgcattt attagacata cggatctccc
    16021 tggaaatttt tttgtgtcgt tgttcgacag atgcgtggaa tggcggaccg aagcgcgtcc
    16081 gctgcaattc gatcgccgga gcggccgcca tcgtatgccc gaatctgcct gccgacaata
    16141 tccaaggcaa cttctgttgc atgcgatgaa atataatccg atttcccatg catgacacaa
    16201 gaggagcgaa cgcgtgaaat tcggcatatt catctacgac ggagtcgagc cgatcgacct
    16261 cgcgacctac ggcgtgctgt cgatggcgcg ccgcgtcgct cccgccatcg aaatcgtcac
    16321 catcgcaccg gcacccggtg tcgtgaacct gtccaacggc ttgcgcgtca ttgccgacca
    16381 cggcatcgac gacgcgccgc gctgcgacgt gctcgtcatc accgggggac cgggatggga
    16441 ggcacagtcg cagtccaccg ccacgatcgc cttcctgcgc gcgcaacccg cggccacccg
    16501 catcgtctcg gtttgcacgg gcgcgatgat cctcgcggca acggggatgc tcgacgggca
    16561 caaggcaacg acgaagtgcc aggtgcggtc gcccgaatcg tcgccgctcg agctgatgcg
    16621 gcagcgccat ccggccatcg acgcgcagtc ggcaagcgtc gtcgattgcg gcgccctgac
    16681 caccggcggc ggcgtcagcc tttgcatcga tacgatgctg cacgtaattg cgcgcgagct
    16741 cggcgccccc gttgccaagg aaacggcgcg aatcctcgaa tactcgcgcg catgggacgc
    16801 gaaccgccag caat
//
LOCUS       DASSVX010000280         7589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_845653, whole genome shotgun sequence.
ACCESSION   DASSVX010000280 DASSVX010000000
VERSION     DASSVX010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845653"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFO53_12110"
     CDS             complement(<1..190)
                     /locus_tag="VFO53_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HET8877065.1"
                     /translation="MRHARLLLAALFALVSLAAPALAQTALKMNISLAQNSHYGVAID
                     TFAREVAKRTNGRYTIQNF"
     gene            438..1892
                     /locus_tag="VFO53_12115"
     CDS             438..1892
                     /locus_tag="VFO53_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET8877066.1"
                     /translation="MLDPTFTSALAALVPPSALLTEAEDTRPYECDGLTLYRELPAAV
                     VLPENEAQVVAILKLCHAARVPVVARGAGTSLSGGSLPVAAGVVLSLAKFRRIVDIDP
                     LARTAIVEPGVRNLAISEAVAPHGLFYAPDPSSQIACSIGGNVAENAGGVHCLKYGLT
                     VHNVRKVRAVLITGDIVEFGADALDAPGYDLLALVTGSEGLLAVITQITVKLLPKPEL
                     ARVALAAFDDIEKAGAAVGNVIAAGIIPGGLEMMDRDATRAVEQFVHAGYPLDAAAVL
                     LIEADGTPVEVAAEMAEIIDVLTASGATEIRVSADETQRLLYWSGRKAAFPAVGRISP
                     DYYCIDGTIPRAALPGVLMQIETWSREYGLPCANVFHAGDGNLHPLILFDANKPGDRD
                     RTVEFGDRILELCINVGGTITGEHGVGVEKLRGMCAQFAPAALERFLGIKRAFDAHGL
                     LNPGKAVPTLARCAEFGRMHVHHGALPHPDLPRF"
     gene            1913..3325
                     /locus_tag="VFO53_12120"
     CDS             1913..3325
                     /locus_tag="VFO53_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET8877067.1"
                     /translation="MNSTTQTHARPAAEDVTVVTERLVRDYGERAVTSHAIREQHSHG
                     EGLADAGMPDIVVFPKTNEEVASIVRVCNAARIPVIAFGVGTSLEGHVAALYGGVCID
                     LSQMDRVLEVNDADLDCRVQAGVTREALNAELKGTGLFFPIDPGANATLGGMASTRAS
                     GTNAVRYGTMRENVLGLTVVTADGRIVHTGGRARKSSAGYDLTRLFVGSEGTLGVITE
                     VQLRLYGLPEAISAAVCQFADLAGAVDTVIVAMQLGIPVARIELLDDVQMDACIRYSQ
                     LDEFEVRPTLFFEFHGTEAGVREQAETIESIAADHGGSRFRWATAQEERSRLWKARHN
                     AYYAALALAPGTQAFATDACVPISRLTECVLETRADVASTGLIAPIVGHVGDGNFHLV
                     VLFDPAQPGQRAKAEGLAARVSARAIRMGGTCTGEHGIGVHKLDALVAEHGDAVSLMQ
                     AVKRALDPNDILNPGKTVPR"
     gene            3329..4000
                     /locus_tag="VFO53_12125"
     CDS             3329..4000
                     /locus_tag="VFO53_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET8877068.1"
                     /translation="MRLYIGNRNYSSWSLRGWLVTRLAGEPFEEIAVPLAGLVPNPAN
                     YAFSPSGLVPCLHDGDAVVWDSLAIAGHLAERHPNMWPDEANARAWARSISAEMHSGF
                     ATLRSDMTMCIRERVDVRPWSPALASNIERVIRIWEESRRRFGAGGAFLCGRYSIADC
                     FYAPVAFRFRTYAVAPGGAAGAYLDALLAHPDVREWERSALAETAVVEADEPRHLYRD
                     KLAAA"
     gene            4010..5086
                     /gene="glcE"
                     /locus_tag="VFO53_12130"
     CDS             4010..5086
                     /gene="glcE"
                     /locus_tag="VFO53_12130"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HET8877069.1"
                     /translation="MEDIAVARLEDRIRAAVADRRALRIHGGGTKDFYGDALVGDPLD
                     VRPVAGIVSYAPGELVLTVRAGTPLAEVESALAAHGQMLAFEPPCHGEATTLGGIVAT
                     GFSGPRRPHAGAARDFVLGTRVVDGTGASLAFGGQVIKNVAGFDVSRLMTGALGTLGV
                     ITEISLKCLPRPKVEATRIVQCDGPGALRLVNQWGGKALPISATCFHREELHVRLSGA
                     SAAVTSAAAIIGGDEVDAVDFWRAVRDQTLDYFRPATGADATLWRLSVRSTAPWTAFA
                     GDTLVEWGGAQRWLVCKTPLEPESVRAWASEHGGHATLFRAANKSAGTFQQLPAALRA
                     LHERLKQVFDPHRVLNPGRMYAAL"
     gene            5086..6375
                     /gene="glcF"
                     /locus_tag="VFO53_12135"
     CDS             5086..6375
                     /gene="glcF"
                     /locus_tag="VFO53_12135"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HET8877070.1"
                     /translation="METKLLPSVAATPEGKEAESILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQALEGAEVTAKTQLHLDRCLTCRACETTCPSGVRYGRLVDIGRKFV
                     DDRVGRSAMDAARRGTLRRSLLSRPLFGAALRLGRFAKGWLPQRLASSIPDVHSAGAW
                     PAPRHARRVLIPAGCVQPSLAPNIDAATARVLDRAGISAVAVDDGGCCGALSYHLSEH
                     DEARAFARRNIDAWWPHVEQGAEAIVVTASGCGVMVREYDHLLAGDPAYAVRARRMAG
                     LARDPVELIAGAWPHVAPQIDRERTGARVAFHPPCTLQHGIGIRGAVERLLADAGFTL
                     LPVTDAHLCCGSAGTYSILQPELAGRLKANKLHALEANVPDVIATANIGCMTHLQSGA
                     SVPVRHWVELLDAALRGDARSHAEGDVRTLRDPATIG"
     gene            6372..6857
                     /locus_tag="VFO53_12140"
     CDS             6372..6857
                     /locus_tag="VFO53_12140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET8877071.1"
                     /translation="MSSAPASADERNAALAPSRRGDYPSFLAIATRWMDNDTYGHVNN
                     VTYYAYFDTVVNEHLIRAGGLDIRNGPAIGIVVETACRFHKPLSFPDIVDAGMRVAAI
                     GRSSVRYEIALFCQGDDEPAATGRFVHVWVDRAHRRPVSVPAPIRAALEPLRVGSAAL
                     P"
     gene            6854..7384
                     /locus_tag="VFO53_12145"
     CDS             6854..7384
                     /locus_tag="VFO53_12145"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8877072.1"
                     /translation="MSAAGHPRQSVVCEPMTAATRTHARRLLGGFLDTDAHYRASAAQ
                     YGDGGAEALGRALDLFVERPEIGFVWLARTGDSVAGACVVCYAISTSLGGLVAKLDDV
                     TVDPKRHNQGIGTAMLAALIRHLRERGVGRIDTACHRENIAAWRFYQRHGFRPLDEER
                     MALLIDESSSSQGPTT"
     gene            7381..>7589
                     /locus_tag="VFO53_12150"
     CDS             7381..>7589
                     /locus_tag="VFO53_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET8877073.1"
                     /translation="MNKVFPFAGLVLSLGVTAPGALAQSADPIPMRDFFRHPDRAYYR
                     ISGDGRTLSFMQPWERRMNIYVQPVG"
BASE COUNT         1142 a         2612 c         2606 g         1229 t
ORIGIN      
        1 agaagttctg gatcgtgtag cggccattcg tgcgcttcgc gacctcgcgc gcgaacgtgt
       61 cgatcgccac gccgtagtgc gaattctgcg cgagcgagat gttcatcttc agcgccgtct
      121 gcgcgagggc aggcgcggcg agcgaaacga gcgcgaagag cgcggcgagc agcaaacggg
      181 cgtgacgcat cgtgaattct ccgaaaaatc gcgccggccg gcacaccggc ttcgagcgcg
      241 ggggcgattc tagcaggcgc ccttcggcga atcggcgccc gcgaggtgca atatggatgc
      301 aaacggcagc gttgcaatgc gctcgcgcgg caggcatcca cgccattgat ggacgcgttg
      361 ggcgaccggc gcaacggtgc tacgcgcgca ctatgaaata atcacccgat ggcgcgtgcg
      421 accgcgcgct acaccccatg ctcgatccga cgttcacttc ggcgctcgcc gcgctggtgc
      481 cgccctcggc gctcctcacc gaggccgaag acacgcgccc ttacgaatgc gacggcctga
      541 ccctctaccg cgagctgccg gccgccgtcg tgctgccgga gaacgaggcg caggtcgtgg
      601 cgatcctcaa gctttgccat gcggcccgcg tgccggtcgt tgcgcgcggc gccggcacga
      661 gcctgtccgg cgggtcgctg ccggtcgccg ccggcgtcgt gctctcgctc gccaagttcc
      721 gccgcatcgt cgacatcgat ccgctggcgc gcacggcgat cgtcgagccc ggggtgcgca
      781 atctcgcgat ctccgaggcg gtggcgcctc acggtctctt ctacgctccc gatccgtcgt
      841 cgcagatcgc ctgctcgatc ggcggcaacg tcgcggagaa cgccggcggc gtgcattgcc
      901 tcaagtacgg gctcaccgtg cataacgtgc gcaaggtacg cgcagtactg atcaccgggg
      961 acatcgtcga attcggcgcc gatgcgctcg acgcgcccgg ctatgacctg ctggcgctcg
     1021 tcaccggcag cgaaggcctg ctcgccgtca tcacgcagat cacggtcaag ctgctgccga
     1081 agcccgagct cgcgcgggtg gcactcgccg cgttcgacga catcgagaag gcgggtgccg
     1141 cggtcggcaa cgtgatcgcc gccggcatca tccccggcgg ccttgaaatg atggatcgtg
     1201 acgcgacgcg cgccgtcgag caattcgtcc acgccggcta cccgctcgac gccgccgcgg
     1261 tgctgctgat cgaggccgac gggacgccgg tcgaagtggc cgccgagatg gccgagatca
     1321 tcgacgtgct gacggcgagc ggcgccaccg aaatccgcgt ttcggccgac gaaacgcagc
     1381 gccttttgta ctggtcgggg cgcaaggcgg cgtttccggc ggtcgggcgc atctcgcccg
     1441 actactactg catcgacggc acgatcccgc gcgcagcact ccccggcgtg ctgatgcaga
     1501 tcgagacgtg gtcgcgcgag tacggattgc cctgcgccaa cgtgttccac gccggcgacg
     1561 ggaatctgca tccgctgatc ctgttcgacg cgaacaagcc gggggatcgc gatcgcaccg
     1621 tcgagttcgg cgatcgcatt ctcgagctgt gcatcaacgt gggcggcacg atcaccggcg
     1681 agcacggcgt gggcgtcgag aagctccgcg gcatgtgtgc gcagttcgcg cccgcggcgc
     1741 tcgagcgttt cctcggcatc aagcgggcgt tcgacgcgca cggcctgctc aatcccggca
     1801 aggcggtgcc aacccttgcc cgctgcgccg agttcggccg gatgcacgtc caccacggcg
     1861 cgctgccgca tcccgacctg ccgcggttct gaatggcgaa cgcatcgaga cgatgaattc
     1921 caccacgcaa acgcatgcac ggcccgccgc cgaggacgtg acggtcgtga ccgagcgcct
     1981 cgtgcgcgac tacggcgagc gtgcggtgac gagccacgcg atccgagagc agcactcgca
     2041 cggcgaaggc cttgccgacg ccgggatgcc cgacatcgtc gtgtttccga agacgaacga
     2101 agaggtcgcg tcgatcgtgc gcgtgtgcaa cgccgcgcgc attccggtga tcgcgttcgg
     2161 cgtcggcacg tcgctcgaag gccatgtcgc cgcgctgtac ggtggcgtgt gcatcgatct
     2221 ttcgcagatg gatcgcgtgc tcgaagtgaa cgacgcggac ctcgactgtc gcgtgcaggc
     2281 cggcgtgacg cgtgaggcgc tgaacgccga gctcaagggc accgggctct tcttcccgat
     2341 cgatccgggc gccaacgcga cgctcggcgg catggcgtcg acacgcgcgt cgggaaccaa
     2401 tgcggtgcgc tacggcacga tgcgcgagaa cgtgctcggc ctcaccgtcg tcacggccga
     2461 cggccgcatc gtgcataccg gcggccgcgc gcgcaagtcg agcgcgggtt acgacctcac
     2521 gcgacttttc gtcgggtccg aaggcacgct cggcgtgatc accgaagtac agcttcgcct
     2581 gtacggcttg cccgaggcga tctccgctgc cgtgtgccag ttcgccgatc tcgccggcgc
     2641 cgtcgacacg gtgatcgtcg cgatgcagct cggcattccc gtcgcgcgca tcgagttgct
     2701 cgacgacgtg cagatggatg cgtgcattcg ctactcgcag ctcgatgagt tcgaggtccg
     2761 gccgacgctc tttttcgagt ttcacggcac cgaggccggc gtgcgcgagc aggccgaaac
     2821 gatcgaatcc atcgccgccg accacggcgg cagccgattc cggtgggcga cggcgcagga
     2881 agagcgctcg cggctgtgga aggcccggca caacgcgtat tacgccgcac tcgcgcttgc
     2941 gccgggcacg caggcgttcg cgaccgacgc atgcgtgccg atatcgcggc tcaccgaatg
     3001 cgtgctcgaa acgcgcgccg acgttgcatc caccgggttg atcgcgccga tcgtcggtca
     3061 cgtcggcgac ggcaacttcc acctggtcgt gctgttcgac cccgcgcagc cgggccagcg
     3121 cgcgaaagcg gaagggctgg ccgcgcgcgt cagcgcacgc gcgatccgga tgggcggcac
     3181 gtgcaccggc gagcatggca tcggcgtgca caagctcgac gcgctcgtgg ccgagcatgg
     3241 cgacgcggtg tcgctgatgc aggccgtgaa gcgggcgctc gatccgaacg acattctcaa
     3301 cccgggaaaa acggttcccc gctagatcat gagactctac atcggcaaca ggaattattc
     3361 gtcgtggtcg ctgcgcggct ggctcgtcac gaggcttgcc ggcgagccgt tcgaggaaat
     3421 cgcggtcccg ctcgccgggc tcgttcccaa tccggcgaac tacgcgttct cgccgtcggg
     3481 gcttgtcccg tgcctccacg atggcgatgc ggttgtctgg gattcgcttg cgatcgccgg
     3541 ccatcttgcc gagcgccatc cgaacatgtg gcccgacgag gcgaatgcgc gcgcatgggc
     3601 gcgctcgatc agcgccgaaa tgcactcggg attcgcgaca ctgcgcagcg acatgacgat
     3661 gtgcatccgc gagcgtgtcg acgtccggcc gtggtcaccc gcgctcgcgt cgaacatcga
     3721 gcgcgtcatc cgcatctggg aggagtcgcg ccgccggttc ggcgccggtg gcgctttcct
     3781 ctgcggccgc tactcgatcg ccgactgctt ctatgcgccg gtcgcgttcc gcttccgtac
     3841 ctatgccgtc gcgcccggcg gcgcggcggg cgcgtacctt gacgcgttgc tcgcgcatcc
     3901 cgatgtgcgg gagtgggagc gaagcgcgct cgccgagacg gcggtcgtcg aggcggacga
     3961 gccgcgccat ctgtaccgcg acaagctcgc cgccgcctga gtcggattcg tggaagacat
     4021 cgccgttgcc cgactcgagg accgcattcg cgcggccgtc gcggatcggc gcgcgctgcg
     4081 catccatggc ggtggcacga aggacttcta cggtgatgca ctcgtcggtg acccgctcga
     4141 cgtgcgaccg gtcgccggca tcgtgtcgta tgcaccgggt gagctcgtgc tgaccgtgcg
     4201 cgccggcacg ccgctcgccg aagtcgagag cgcgctcgcc gcgcatgggc agatgctcgc
     4261 attcgagccg ccctgtcacg gcgaagcaac gacgctcggc ggaatcgtcg caacgggctt
     4321 ctccgggccg cggcgtccgc acgccggtgc ggcacgcgac ttcgtcctcg gcacgcgcgt
     4381 cgtcgacgga accggggcct cgcttgcatt cggcggacag gtgatcaaga acgtcgccgg
     4441 cttcgacgtg tcgcgactga tgaccggcgc gctgggcacg ctcggcgtca tcaccgaaat
     4501 ctcgctcaaa tgcctgccgc gtccgaaggt cgaggcgacc cgcatcgtgc aatgcgacgg
     4561 gccgggagcg ctgcgcctgg tgaaccaatg gggcggcaag gcgcttccca tttccgcaac
     4621 ctgctttcat cgcgaggagc ttcatgtgcg cctgtccggc gcgagcgccg ccgtgacgag
     4681 cgccgcagcg atcatcggcg gcgacgaagt cgacgcggtg gatttctggc gcgcggttcg
     4741 cgatcagacg ctcgactatt tccggcctgc aaccggcgcc gatgcgacgc tgtggcggct
     4801 gtccgttcgc tcgaccgcac cgtggaccgc gttcgcgggc gatacgctgg tcgaatgggg
     4861 cggcgcgcaa cgctggctcg tgtgcaagac gccactcgag cccgaatcgg tgcgcgcatg
     4921 ggcgtccgag cacggcgggc atgcgacgct gtttcgcgca gcgaacaagt cggccggcac
     4981 ctttcagcag ctcccggcag cgctgcgtgc gctgcacgag cggctgaagc aggtgttcga
     5041 cccgcaccgc gtcctcaacc cgggccgcat gtacgcggcg ctgtgatgga aaccaagctg
     5101 ctgccgtcgg tcgccgcgac accggaaggc aaggaggccg agtcgatcct gcgcgcctgc
     5161 gtgcattgcg gcttctgcac ggcgacgtgt ccgacctacc agcttctcgg cgacgagctc
     5221 gacgggccgc gcgggcgcat ctacctcatc aagcaggcgc tcgagggcgc cgaagtgacc
     5281 gcgaagacgc agctgcatct cgatcgctgc ctcacctgcc gtgcctgcga gacgacctgt
     5341 ccgtcggggg tgcgctatgg acggctcgtc gacatcggcc gcaagttcgt cgacgaccgt
     5401 gtcggtcgca gtgcaatgga cgcggcgcgc cgcggcacgc tgcggcgaag tctcctctca
     5461 cggccgctct tcggcgcagc actgcgtctc gggcgattcg ccaaagggtg gttgccgcag
     5521 cggctcgcgt cgtcgattcc cgacgtgcac agcgcgggcg cgtggccggc accacgccac
     5581 gcgcgccgcg tgctgatccc ggccgggtgc gtgcaaccgt cgctcgcgcc gaacatcgac
     5641 gccgcaacgg cgcgcgtgct cgaccgcgca ggcatctcgg cggtcgcggt ggatgacggc
     5701 ggctgctgtg gcgcgctgtc gtatcacctg tccgagcacg acgaggcacg cgcgttcgcg
     5761 cggcgcaaca tcgacgcgtg gtggccgcat gtcgagcagg gtgccgaggc gatcgtcgtc
     5821 acggcgagcg gctgcggcgt gatggtgcgt gaatacgacc acctgctcgc cggcgatccg
     5881 gcgtatgcgg tgcgggcgcg gcgcatggcc gggctcgccc gcgatccggt cgagctcatc
     5941 gccggcgcgt ggccgcacgt tgcgccgcag atcgaccgcg agcggaccgg cgcacgcgtt
     6001 gcgttccatc cgccgtgcac gctgcagcac gggatcggca ttcgcggcgc agtcgagcgc
     6061 ctgctcgccg atgccggatt cacgctgctg ccggtcaccg acgcgcattt gtgctgcggc
     6121 tcggcgggca cgtattcgat cctgcagccc gagcttgccg gcaggctcaa ggcgaacaag
     6181 ctgcacgcgc tcgaggcaaa cgttcccgac gtgatcgcca cggcgaacat cggctgcatg
     6241 acgcacctgc agtcgggcgc atcggttccg gtgcgtcact gggtcgagct gctcgacgcg
     6301 gcgctgcgcg gcgacgcgcg cagccacgcg gagggcgatg tgcgaacgct gcgcgacccc
     6361 gccacgatcg gatgagcagc gcaccggcgt ccgcggacga gcgcaacgcg gcgcttgcgc
     6421 cgtcgcggcg cggcgactat ccgtcgtttc tcgcgattgc aacgcgctgg atggacaacg
     6481 acacctacgg ccacgtcaac aacgtcacgt actacgcgta tttcgacacc gtcgtgaacg
     6541 agcacctgat ccgcgcaggc ggcctcgata tccgcaacgg gcccgcgatc ggcatcgtcg
     6601 tcgagaccgc gtgccgcttc cacaagccac tgtcgtttcc cgacatcgtc gatgcgggga
     6661 tgcgcgtggc cgccatcggc cgctcgtcgg tgcgctacga gatcgcgctc ttttgccagg
     6721 gcgacgacga gcccgcggcc accgggcgct ttgtccatgt ctgggtcgat cgcgcgcacc
     6781 ggcgtccggt gtcggtgccg gcgccgatcc gcgccgcgct cgagccgctt cgtgtcggca
     6841 gcgcggcctt gccatgagcg cggccgggca tccgcgtcaa agcgtcgtct gcgagccgat
     6901 gacggccgcg acgcgcacgc atgcgcgacg tctgctcggc ggcttcctcg acaccgatgc
     6961 gcattaccgc gcctcggcgg cgcagtatgg cgacggcgga gcggaagcgc tcgggcgggc
     7021 gctcgatctg tttgtcgagc gtccagaaat cggcttcgtg tggctcgcgc gcaccggtga
     7081 ttcggtcgcc ggcgcgtgcg tcgtgtgcta cgcgatttcg acgtcgctcg gcggcctggt
     7141 cgcgaagctc gacgacgtga ctgtcgatcc aaagcggcat aaccagggca tcggcacggc
     7201 catgctggcc gcgctgattc gtcatttgcg cgagcgcggc gtcggacgca tcgacaccgc
     7261 ttgccatcgc gagaacatcg ccgcctggcg tttctaccag cggcacggct tccgtccgct
     7321 cgacgaggaa cgcatggcgc tgctcatcga cgaatcgtct tcttcgcaag gaccaacgac
     7381 atgaacaagg tctttccgtt cgccggtctc gtgctgtcgc tcggcgtcac ggcacccggc
     7441 gcacttgccc aatcggccga tccgattccg atgcgcgact tcttccgtca tccggatcgt
     7501 gcgtactacc gcatctccgg cgatggcagg acgctgtcgt tcatgcagcc gtgggagcgg
     7561 cggatgaaca tttacgtgca accggtcgg
//
LOCUS       DASSVX010000281         6476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1045694, whole genome shotgun sequence.
ACCESSION   DASSVX010000281 DASSVX010000000
VERSION     DASSVX010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045694"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VFO53_12155"
     CDS             complement(<1..744)
                     /locus_tag="VFO53_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HET8877074.1"
                     /translation="MTLVHIVIAALAGGVVSTVAAGATLNLDARWIPGFVSFAVGALL
                     GAVFLELLPHALEGGHAANVMVTVLIGLLAFFLLEKLVLWRHAHGHAAHSDAREETEH
                     EHALHAQHVAEGRPGLMILIGNSVHSFCDGIVIAAAFLADDALGVATTLAIVAHAVPQ
                     QVGDFAVLLHSGFARSKAFAYNVATGLATLAGALAAYGALADMQQLLPSVLAIAAASL
                     LYVAVADLIPSLHRRAEPVETAKQMFWIGL"
     gene            complement(863..1699)
                     /gene="pgeF"
                     /locus_tag="VFO53_12160"
     CDS             complement(863..1699)
                     /gene="pgeF"
                     /locus_tag="VFO53_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HET8877075.1"
                     /translation="MNRDARAAAGDVTDLRAPLAQRFAAAGVDWIVPQWSASTRVHAF
                     ATTRNGGTSTGACASLDLGSASLASVKPDAAAAIVANRRRTQAFLPAPPVWLEQVHGA
                     AVVSIGAVPPASPPRADAAVTRATRVVLAVRIADCMPLLFAARDGSVIAVAHAGWRGL
                     AAGILENTVDAMDSDPAGIVAWLGPAIGPAAFEVGADVRDAFVRGDAAARHAFVDASP
                     GKWLADLEALTRMRLARAGVRSIDGGGMCTMSDPSRFFSFRRDGPTGRMAAFVWRGDR
                     CE"
     gene            complement(1696..2721)
                     /gene="rluD"
                     /locus_tag="VFO53_12165"
     CDS             complement(1696..2721)
                     /gene="rluD"
                     /locus_tag="VFO53_12165"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HET8877076.1"
                     /translation="MSAANYSRRSPPADAREGSPQAASLTLSVPAELAGLRLDQALAR
                     MLPAHSRTRLKTWIDAGHVKVDDTTWDARRRVGGAERIDVTAPLLEASVADAAEDIAL
                     SIVHEDDAILVLDKPAGLVVHPGSGNRAGTLLNALLHHDPALASVPRAGIVHRLDKDT
                     SGLMVVARTIEAQTDLVRALAAHRVAREYLAVARGDIDRDSVVDAPIGRHPTQRTTMA
                     VVARGKPARTHVSVVERFGSATLLRCTLETGRTHQIRVHLAAVGHPLVGDPAYGGRRA
                     TRAAAGARDSRAQPLPPFPRQALHATRLSLTHPLTRQSMAWHAAPPADFARLVAALRE
                     MKTSDAR"
     gene            2733..3542
                     /locus_tag="VFO53_12170"
     CDS             2733..3542
                     /locus_tag="VFO53_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET8877077.1"
                     /translation="MTMSQWPKWQRWLWMALLALTAAGCGLLPDVKDETAGWSAEQLY
                     KNAHEAMQDGNYTRAVKLFDTLEARYPYGRFAQQAILEGAFSNWRNSEQAAATAACDR
                     FIRTYPNHPNVDYAYYLKGLVYFREDQGLFGYVYELDLSERDPKQMRESFAAFKELVQ
                     KFPRSNYAEDAQIRMRYLTNALGMYEVHVASYYYARGAYVAAANRAQASLVNHPRTPS
                     NEMALDLMVRSYRKLGLTQLADDAHAILEKTFPSSVYLAANAPAKPWWKVW"
     gene            complement(3577..5574)
                     /locus_tag="VFO53_12175"
     CDS             complement(3577..5574)
                     /locus_tag="VFO53_12175"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET8877078.1"
                     /translation="MPNDASTDASHAELAIILGENGTLARALPGFRFRPQQLAMAQAV
                     AHAINHREPLVAEAGTGTGKTFAYLVPALLYGGKVIVSTGTKTLQDQLFERDLPLIRD
                     ALAVPITVALLKGRANYVCHHHLERTAREERLPSREDARYLPKIVAFARASERGDRAE
                     LADVPEHATIWPMVTSTRDNCLGSNCAFYRECFVMKARKDALDADVVVVNHHLFFADV
                     TLRDEGLAELLPACNTVILDEAHQLPDTATLFFGEQTSSSQLAELARDAEAIARTELR
                     DIPDLPDATALLAPLIRKLRLAAGEASGKFAQQALAARPDFVEALAALAAGVDRLAAE
                     MQLVAERSEDVAAIARRIEAAAATLGRWQAGYAGEETSAEPWIRWIDVSATGFQLHAS
                     PLSVADVFRRQVEATGRAWIFTSATLAVGRDFSHYTAQLGLADAATGCWDSPFDYPAQ
                     GLLYVPRGLPAPNSIEHTNAVVDAALPVLKASGGRAFLLFTTLRALNAARERVVALFA
                     RERLDYPVLVQGDGSKTDLLLRFRALGNAVLLGSASFWEGVDVPGDALSVVVIDKLPF
                     APPDDPLLAARLAKLAADGGNAFMHWQVPQAVISLKQGAGRLIRTESDRGVLMLCDPR
                     LVDKPYGKRIWQSLPPMRRTRELDEVIAFFGTAGEVCSAQR"
     gene            complement(5583..6191)
                     /locus_tag="VFO53_12180"
     CDS             complement(5583..6191)
                     /locus_tag="VFO53_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877079.1"
                     /translation="MHARLLLVVPALAAALATSPGHAETIGGNPGPEHNYVCPHSDGK
                     PALDCYFDAVQHLYTMCRNVKSIEIIEFGYDKSTEGTNGAKSESCLDKQKQNIAPPYK
                     AALKEAGKSKPALEALHGLQESWLSAMSGLGWHSGETDGDYKTRVARPYEEFTHRIET
                     IRTSLAEVETKAAAAKAAKATKPKHGATAQPAKSKTAKAKKH"
     gene            complement(6330..>6476)
                     /locus_tag="VFO53_12185"
     CDS             complement(6330..>6476)
                     /locus_tag="VFO53_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8877080.1"
                     /translation="VIKMMEDKVPLGRLGKPEEIANTYAWLASDEASYINGAVIEVSG
                     GCTI"
BASE COUNT         1020 a         2149 c         2300 g         1007 t
ORIGIN      
        1 gaggccgatc cagaacatct gcttcgccgt ttcgacgggc tcggcgcgcc ggtgcaggct
       61 cgggatcagg tcggcgaccg cgacgtacaa caggctggcg gccgcaatgg cgagcaccga
      121 cggcagcagt tgctgcatgt ccgccagtgc gccatacgcc gcgagcgcgc cggcgagtgt
      181 cgcaagaccg gttgcgacgt tgtaggcgaa cgccttgctg cgcgcgaatc ccgagtgcag
      241 cagcacggca aaatcgccga cctgctgcgg caccgcgtgc gcgacgattg caagcgtcgt
      301 cgcgacgccg agcgcgtcgt cggcgagaaa ggcggcggcg atcacgatgc cgtcgcagaa
      361 gctgtgcacg ctgttgccga tcaggatcat gaggcccggg cgtccctcgg caacgtgctg
      421 cgcatgcagc gcgtgctcgt gctcggtttc ctcgcgcgcg tcggaatgcg cggcatggcc
      481 gtgcgcgtgg cgccacagca cgagcttttc gagcaggaag aacgcaagga gcccgatcag
      541 caccgtcacc atgacattcg cggcgtggcc gccttcgagt gcgtgcggca gaagctcgag
      601 gaataccgcg ccgagcagcg ctccgacggc gaacgacacg aagcccggta tccagcgcgc
      661 gtcgaggttg agcgtcgcgc cggcggcgac cgtcgacacg acgccgccgg cgagtgctgc
      721 gatgacgatg tgaacgagcg tcatggtggg ccccccacgc ttgcggcctc attggtcaat
      781 tcggccgctg cgctgccccc ggcgggggcg caattcgcgt cttggggcgg cccggcgacg
      841 cgaatcggaa cgcgcttcgc gttcattcgc agcgatcccc gcgccagacg aacgccgcca
      901 tgcggccggt cggcccgtcg cggcgaaagg aaaagaagcg cgacgggtcg ctcatcgtgc
      961 acatcccgcc gccatcgatc gaccgtacgc cggcgcgcgc cagacgcatg cgggtgagcg
     1021 cttcgagatc ggccagccat tttcccggtg acgcatcgac gaacgcgtgg cgcgcggcgg
     1081 cgtcgccgcg gacgaacgcg tcgcgcacgt cagcgccgac ttcgaaggca gcaggtccga
     1141 tggcgggccc gagccacgcc acgatgccgg ccggatcgct gtccatcgcg tcgacggtgt
     1201 tctcgaggat gccggccgcg aggccgcgcc aaccggcatg cgcgacggcg atgaccgatc
     1261 cgtcgcgggc ggcgaagagt agcggcatgc agtcggcgat gcgcaccgcg agcacgacac
     1321 gcgtcgcgcg cgtaaccgcc gcatcggcgc gcggcgggct cgccggcggc acggcgccga
     1381 tcgacacgac cgctgcccca tgcacctgtt cgagccagac cggaggtgcc gggaggaacg
     1441 cctgcgtccg gcggcggttc gcgacgatgg ccgcggcggc gtcgggcttc accgatgcga
     1501 gcgacgcgct accgagatcg agcgaagcgc acgcaccggt gctcgtgccg ccattgcgcg
     1561 ttgtcgcgaa ggcatgcacg cgcgtcgatg cggaccactg cggcacgatc cagtccacac
     1621 ctgccgcggc aaaccgttgc gcaagcggcg cgcgcagatc ggtcacgtcg ccggctgccg
     1681 cgcgcgcatc gcgattcatc gagcgtcgct cgtcttcatt tcgcgcagcg ccgcgacgag
     1741 gcgggcgaaa tcggcaggcg gcgcggcatg ccaggccatc gattggcgcg tcagcgggtg
     1801 cgtgagcgaa aggcgcgtcg cgtgcagcgc ctggcgcggg aacggcggca acggctgcgc
     1861 gcgcgaatcg cgtgcgccgg cggcggcgcg cgttgcgcgg cgcccgccgt acgcgggatc
     1921 gccgacaagc ggatgtccga ctgccgcaag atgtacgcgg atctgatgcg tccgccccgt
     1981 ttcgagcgtg cagcgcagaa gcgtcgcgct cccgaagcgc tcgacgacgc tgacgtgcgt
     2041 gcgggccggc ttgccgcgcg cgacgacggc catcgtcgtc cgctgcgtcg ggtgacgtcc
     2101 gatcggcgcg tcgacgacgc tgtcgcggtc gatgtcaccg cgcgcgacgg cgagatactc
     2161 gcgggcgacc cggtgcgccg cgagcgcacg cacgagatcg gtctgcgcct cgatcgttct
     2221 cgcgacgacc atcaaaccgc tcgtgtcctt gtcgaggcga tgcacgatgc ctgcgcgcgg
     2281 aaccgacgca agcgcgggat cgtgatgcag cagcgcgttc agcaacgtgc cggcgcggtt
     2341 gccgctgccg ggatgcacga ccaggccggc gggcttgtcg agcacgagga tggcatcgtc
     2401 ttcgtgcacg atcgacagcg caatgtcctc ggcggcgtcg gcaaccgacg cctcgagcag
     2461 cggcgcggta acgtcgatgc gctcggcgcc gccgacgcgc cggcgtgcat cccatgtcgt
     2521 gtcgtcgacc ttcacgtgac ctgcgtcgat ccacgtcttg agccgggtgc gcgaatgcgc
     2581 gggcagcatg cgcgcaagcg cctggtcgag gcgcagcccg gcgagttcgg cgggcaccga
     2641 aagcgtgagc gacgcagcct gcggcgagcc ctcgcgcgcg tcggccggcg gcgatcgtcg
     2701 gctataattc gcggcactca cgaaggatgc cgatgacgat gtcgcaatgg ccgaaatggc
     2761 agcggtggct ctggatggcg cttctggcgt tgacggcggc cgggtgcgga ctgcttcccg
     2821 acgtcaagga cgaaaccgcc ggatggagcg ccgaacagtt gtacaagaac gcccatgagg
     2881 cgatgcagga cggcaattat acgcgggcgg tcaagctgtt cgacacgctc gaggcgcgct
     2941 atccctacgg ccgcttcgca cagcaggcga tcctcgaggg cgcgttctcg aactggcgca
     3001 acagcgagca ggcggcggca acggcggcgt gcgaccgctt catccgcacg tacccgaacc
     3061 atccgaatgt cgactacgcg tattacctga aggggctcgt gtatttccgc gaggaccagg
     3121 gcctcttcgg ttacgtctac gagctcgacc tgtccgagcg cgatccgaag cagatgcgcg
     3181 aatcgttcgc cgcgttcaag gagctcgtgc agaaattccc gcggagcaac tacgccgagg
     3241 atgcgcagat caggatgcgc tacctcacca acgccctcgg catgtacgag gtgcacgtgg
     3301 cgagctacta ctacgcacgc ggcgcctacg tggcggccgc caaccgcgcg caggcgtcgc
     3361 tcgttaacca cccgcgcacg ccttcgaacg aaatggcgct cgacctgatg gtcaggagct
     3421 atcgcaagct cgggctcacg cagcttgccg acgacgcgca cgcgatcctc gagaagacgt
     3481 ttccgagcag cgtctatctt gcggccaatg cgccggccaa gccgtggtgg aaggtctggt
     3541 agcgcgaccg ggccggaaaa aagccgcgcc gcgcgctcat cgctgcgccg agcacacttc
     3601 cccggcggtg ccgaagaacg cgatcacctc gtcgagctcg cgcgtgcgac gcatcggcgg
     3661 caggctctgc cagatccgct tgccgtaggg cttgtcgaca agacgcgggt cgcacagcat
     3721 cagcacgccg cgatcgcttt cggtgcggat caaccgcccg gcgccctgct tcaggctgat
     3781 caccgcctgc ggcacctgcc agtgcatgaa tgcattgccg ccatcggcgg cgagcttggc
     3841 gaggcgggcg gcgagcagcg gatcgtcggg cggcgcgaac gggagcttgt cgatgacgac
     3901 gacggacagc gcatcgccgg gcacgtcgac gccttcccag aagctcgcgc tgccgaggag
     3961 cacggcattg ccgagcgcgc gaaagcgcag cagaagatcg gttttcgagc cgtcgccctg
     4021 caccagcacc ggatagtcga gtcgctcgcg tgcgaagagc gcaacgaccc gctcgcgcgc
     4081 ggcgttgagc gcacgaagcg tcgtgaacag caggaatgcg cgtccgccgc tcgccttcag
     4141 cacggggagc gccgcatcga ccaccgcgtt cgtatgctcg atgctgttcg gtgccggcag
     4201 ccctcgcggc acatagagca gtccctgcgc cggatagtcg aacgggctgt cccagcagcc
     4261 ggtggcggcg tcggcgagac cgagttgcgc cgtgtaatgc gaaaaatcgc ggccgaccgc
     4321 aagggttgcc gacgtgaaga tccacgcacg gcccgtcgcc tcgacctggc ggcggaacac
     4381 gtcggcgacc gacagcggcg atgcatgcag ctggaacccc gtcgcgctga cgtcgatcca
     4441 gcggatccag ggctcggcgc tcgtttcttc gcccgcgtag ccggcctgcc agcggccaag
     4501 ggttgccgcc gcggcttcga tgcggcgcgc gatcgcggcg acgtcctcgc tgcgctcggc
     4561 cacgagctgc atttcggcgg cgagccggtc gacgccggcg gcaagtgctg cgagcgcctc
     4621 gacgaaatcg gggcgcgccg cgagcgcctg ctgcgcgaac ttgccgctgg cttcaccggc
     4681 cgcgagccgc agcttgcgga tcagcggtgc aagcagcgcc gtcgcatcgg gaagatcggg
     4741 aatgtcccgc agctcggtgc gtgcgatggc ctcggcgtcg cgcgcgagct cggcgagctg
     4801 gctcgagctc gtctgttcgc cgaagaacag cgttgcggtg tcggggagct gatgcgcttc
     4861 gtcgaggatc accgtgttgc aggcgggaag gagctcggcg aggccctcgt cgcgcaacgt
     4921 cacgtcggcg aagaacaggt ggtggttgac gaccacgacg tcggcatcga gcgcatcctt
     4981 gcgtgccttc atcacgaagc attcgcggta gaacgcgcaa ttgctgccga ggcagttgtc
     5041 gcgcgtggac gtgaccatcg gccagatcgt cgcgtgctcg ggcacgtcgg cgagctcggc
     5101 gcgatcgccg cgctcgctcg cgcgtgcaaa cgcgacgatc ttcggcaggt agcgcgcatc
     5161 ctcgcgcgac gggagccgct cctcgcgtgc cgtgcgctcg agatggtgat ggcacacgta
     5221 attcgcgcgc cccttgagca gcgcgacggt gatcgggacg gcgagcgcat cgcggatcag
     5281 cggcaggtcg cgctcgaaga gctgatcctg aagcgtcttc gttcccgtcg acacgatgac
     5341 cttgccgcca tacaacagcg cggggacgag atacgcgaac gtcttgccgg tgcccgtacc
     5401 ggcctcggcg acgagtggct cgcggtgatt gatggcgtgc gcgacggcct gcgccatcgc
     5461 gagctgctgc gggcggaagc ggaaacccgg cagcgcgcgc gcgagcgttc cgttctcgcc
     5521 gaggatgatt gcgagttcgg cgtgcgacgc gtcggtggac gcgtcattcg gcaacggcgc
     5581 gttcagtgct ttttcgcctt cgcggttttg gacttggccg gctgcgcggt cgcgccgtgc
     5641 ttcggcttcg tggctttcgc cgccttggcg gcagcggcct tggtttcgac ctcggcgagg
     5701 ctcgtgcgaa tcgtctcgat gcggtgcgtg aattcctcgt agggtctcgc gacgcgcgtt
     5761 ttgtagtcgc cgtcggtttc gccgctgtgc cagccgagtc cgctcatcgc cgacagccag
     5821 ctttcctgga ggccgtgcag cgcttcgagt gccggcttcg atttgccagc ctccttgagc
     5881 gccgccttgt aggggggcgc gatgttctgc ttctgcttgt cgaggcacga ctcgcttttg
     5941 gcgccgttcg tgccttcggt ggatttgtcg tagccgaact cgatgatctc gatcgacttc
     6001 acgttgcggc acatcgtgta caggtgctgc acggcgtcga aatagcagtc gagcgccggc
     6061 ttgccgtcgg agtgcggaca gacgtagttg tgctcgggtc cggggttgcc gccgatcgtt
     6121 tcggcgtggc cgggcgacgt ggcgagcgcg gcggcaagcg caggcacgac gagaaggagg
     6181 cgggcgtgca tcggtgaggc gggcgccgga gaatgcgatg ccgaatgcta cctgcgccgc
     6241 ggggcaggct caaccggacg aatggattag ggcctaacag gccctcgctg tacaactcgg
     6301 aagccgcggc tgaagtttgt cgcggggcgc tagatcgtgc accctcccga gacctcgatg
     6361 acggcgccgt tgatgtacga cgcctcgtcc gaggcgagcc acgcgtacgt gttcgcgatc
     6421 tcctcgggct tgccgagccg gccgagcggc accttgtctt ccatcatctt gatgac
//
LOCUS       DASSVX010000282         7732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1095594, whole genome shotgun sequence.
ACCESSION   DASSVX010000282 DASSVX010000000
VERSION     DASSVX010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095594"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1377
                     /locus_tag="VFO53_12190"
     CDS             88..1377
                     /locus_tag="VFO53_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877081.1"
                     /translation="MKLLSRLMLVLASALLATCVAAGAARAQAVTLDVFYAQPSFARY
                     HEPIAQAFMKAHPDIRIAFRAPAADYDVGHQAMLREAVTNNLPDIYFPGFHLLAELTD
                     VLVPRHQIVALDTLLAGEPAQWRTDNYAAKILDLGKVHGKLYGLAVNASLPVMYFNAE
                     LVKKAGGDPAHMPDTWEGVIALAKKIHETSNVAGMAYNAHEWPDTWLFQAILTQQGVP
                     MLDPTGTKIAFDNDKGLKALTLLRRFVTEGGMQLIGFAASRQLFVAGEIGIFFDTPAR
                     MRQVTDLVGTRFALGTAVFPIDDKATGGIPTGGCAIVITARDPAKQKAAWEYAKFITG
                     PEAQKIVVEATGYLPTNKRAAGPDFLGPFYDKNPNFKTVALETDRAVPWQGYAGASTV
                     QIWRKQREIINVVMRGEITPSAGLERIASETAAMMKK"
     gene            complement(1450..2484)
                     /locus_tag="VFO53_12195"
     CDS             complement(1450..2484)
                     /locus_tag="VFO53_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010437418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8877082.1"
                     /translation="MPDFANGVARLLVVDDNKVNRLLLTRNLDLMGHRVSAAENGRVA
                     LDTLRRDAFDLVLLDIEMPEMDGFQVLEQIRGDLALRDVPVIVTSSLEGIDNVVRCIE
                     LGAEDYLTKPVNAVLLKARVGASLEKKRLRDQQKALVRRFATPEVAQDLQQSGFALGG
                     KRIRASVMFADIRGFTSLAESQPPEETIELLNTYYTLMFDAIGRHGGIVTHMAGDGLM
                     AIFGAPLPLPGHCDAAVRAALEMIETIDLFDVDQRAAGKPEIRIGIGIASGDMVAGYA
                     GTNERATYTCIGDTVNLASRLESHTKLAMHPILIDGDTRAHLHDALSPRPLGPVVLPG
                     RAQPVDVFAL"
     gene            complement(2477..2824)
                     /locus_tag="VFO53_12200"
     CDS             complement(2477..2824)
                     /locus_tag="VFO53_12200"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET8877083.1"
                     /translation="MTQPTIDLAAFGELQSTAGAEFVCELVDTFLVEAPLMLDELRLA
                     YASGNADTFRRTAHSLKSNSNTFGALTLGKLAKDLELGGMAPVREANGAPLEELEFEY
                     ARVAKALAELARA"
     gene            complement(2993..>7732)
                     /locus_tag="VFO53_12205"
     CDS             complement(2993..>7732)
                     /locus_tag="VFO53_12205"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET8877084.1"
                     /translation="FDETQRLLKETERRSSELAVINSIQQGMAKELNFQAIVDVVGDQ
                     LRRMFATGDMTIAWNDYKTATVRPLYVYEHGKRLTLPPRPYDAEKPINRALRTGRPVV
                     LGDRAAMDAIGVTTLEGTDTSLSCVFVPVMVGERLVAVIWIESFEREHAFDDAQVHLL
                     STIAASMGVALENARLLAETQRNARESSALSDVGRDLSSTLDLATVMDRIAGHAKAML
                     SAGNSAIFLPDADGRNYRALVALGDLADQLRASVVEPGHGIIGSLIESGRPELINDTA
                     ADPRALQIAGTEMRRDERLMVVPLLGGETVQGAMAVWRSGGSPFEAHELEFLVGLSRQ
                     AAIALKNAQLYRETQEALEQQTASAEVLQVIGSSMADAQPVFDKIAQSCGQLFASEQV
                     GVFLLGDDGLLHAGTWYGAAMEAVVRTFPKPLEQTITAQVLRERAPVHIADAAALKDA
                     PATLREMQTLIGNFSAVWVPMLWEGRGVGSICLLRQPVKAFSDKELALLKTFADQAVI
                     AVQNARLFNETKEALDQQTATAEILKVISRSPTDVQPVLDAVAEAAGRLCRAEGSRVW
                     LVDGDRLRAMTSYGPADADSRGETLPIRTTSIAGRAVLERRCIHLHDVLPLIETEYPD
                     VRELQARHGFRTVLNVPLLQDGEAIGVIAMLRREVRPFSASEIALVQTFADQAVIAIQ
                     NVRLFKQTQEALDRQTATSDILRVISGSPTDVQPVFDAIVEAALRLLPATFAVLLRRD
                     GAFYRIGATANRDEAQRRRRLDEHPARVPIDPAANFPSRVFVSKAMLHIPDWSAIELP
                     PHERTVFEGTSLHSSLMLPLVCDGDCVGVLAIAHAKAHAYSDGEIALAQSFVDQAVIA
                     IQNTRLFNETKEALEQQTATAEVLQVISSSVSDTAPVFDKILDSCQRLFTAHQLAVML
                     VREDGRVYPAAWRGSAFDTLMRDIGSMPLDATFTGQAIRERRPVRATDQEALAMGYPG
                     IDRLVETVGHYTSVYSPMFWEGRGIGAICVFRQPPRPYTDKEVELLRIFSDQAAIAIQ
                     NARLFNEARDARAAAETANEAKSAFLATMSHEIRTPMNAVIGMSGLLLDTKLDAEQHD
                     YASTIRDSGDALLTIINDILDFSKIEAGRMDIEAHPFDLRECVESALDLVSTRAAEKH
                     LDIAYVFEGDVPAAVNGDVTRLRQVLLNLLSNAVKFTEAGEVVLTVSAGMQDAAHELH
                     FAIRDTGIGLTDAGLARLFRSFSQADSSTTRKYGGTGLGLAISKRLAELMGGTMWAQS
                     AGPGTGSTFFVTIRAEPAALAPNARREFIGTQPALAAKRLLIVDDNATNRRILALQAA
                     KWGMLTRDTQFPAQALQWIADGDAFDVAILDMHMPEMDGIMLATRIRSMRAMLPLVLF
                     TSLGRREAGDAAGLFAATLAKPLRQSQLFDTLMTLLAHEMTDASPRPVATAKPRIDAG
                     MAARHPLRILLAEDNVVNQKLALRLLQQMGYRADLASNGVEAVESVARQTYDVVLMDV
                     QMPEMDGLEASKAITARFRPSERPRIVAMTANAMQGDRELCLAAGMDDYMTKPIRVDA
                     LVEALARVAARGTAEQRADLARLGEEE"
BASE COUNT         1249 a         2600 c         2558 g         1325 t
ORIGIN      
        1 aaatccgtgt accatgcgcc gtgctgcccg gcgtccggct cgcgattcat ccattgcgaa
       61 tggcgacgcc ccatggagga aaggtcgatg aaacttctgt ccaggctgat gctggtgctc
      121 gcatcggcgc tgctcgcgac gtgcgtcgcg gccggcgcgg cgcgcgcgca agccgtgacg
      181 ctggatgtgt tctacgcgca gccgtcgttc gcgcgctacc acgagccgat cgcgcaggcg
      241 ttcatgaagg cgcaccccga catcaggatc gcgtttcgtg cgcccgccgc cgactacgac
      301 gtcggacacc aggcgatgct ccgcgaggcg gtcaccaaca atctgccgga catctacttc
      361 cccgggtttc atctgctggc ggaattgacc gacgtgctgg tcccgcggca ccagatcgtg
      421 gcgctcgaca cgctgctcgc cggcgagccg gcgcagtggc gcaccgacaa ctacgcggcg
      481 aagatcctcg acctcggcaa ggtgcacggc aagctgtacg ggctcgcggt gaatgcgtcg
      541 cttccggtga tgtacttcaa cgccgagctc gtgaaaaaag ccggtggcga tcccgcgcac
      601 atgccggaca cctgggaagg cgtcatcgcg ctggcgaaaa agattcatga gacgtcgaac
      661 gtcgcgggaa tggcgtacaa cgcgcacgaa tggcccgata cgtggctgtt ccaggcgatc
      721 ctcacgcagc agggcgtgcc gatgctcgat cccaccggca ccaagatcgc gttcgacaac
      781 gacaaggggc tgaaggcgct gacgctgctg cgccgcttcg tgaccgaagg cggcatgcag
      841 ctcatcggat tcgccgcgtc gcgccagctc ttcgtcgccg gcgaaatcgg catcttcttc
      901 gacacgccgg cacgcatgcg tcaggtgacc gacctcgtcg gcacgcgctt cgcgctcggc
      961 acggcggtgt tcccgatcga cgacaaggcg acgggcggca tcccaactgg cggctgcgca
     1021 atcgtcatca ccgcgcgcga tcctgctaag cagaaggcgg cgtgggagta cgcgaagttc
     1081 atcaccgggc ccgaagccca gaagatcgtc gtcgaggcga ccggctacct gccgaccaac
     1141 aagcgcgcgg cgggtccgga cttcctcggg ccgttctacg acaagaaccc gaatttcaag
     1201 accgttgcgc tcgagaccga ccgcgcggtg ccatggcagg gatacgccgg cgcgagcacc
     1261 gtgcagatct ggcgcaagca gcgcgaaatc atcaatgtcg tgatgcgcgg cgagatcacc
     1321 cccagcgccg gactcgaacg catcgcgagc gaaaccgcgg ccatgatgaa gaagtgagcg
     1381 cagaggcagc gtcgccgagc gctacaggca cgcgaaagca gcggggcgcg ccgcaggcga
     1441 gcgacgcggt cagagcgcga acacgtcgac cggctgcgcc ctgcccggaa gcacgaccgg
     1501 accgagggga cgcggcgaca gcgcatcgtg caggtgcgcg cgggtgtcgc cgtcgatcag
     1561 gatcgggtgc atcgcgagct tcgtatgcga ttcgaggcgc gatgcgaggt tgaccgtatc
     1621 gccgatgcac gtgtaggtcg cgcgctcgtt cgtccctgcg tagccggcca ccatgtcgcc
     1681 ggatgcgatt ccgatgccga tgcggatttc cggcttgccc gcggcacgct ggtcgacgtc
     1741 gaagaggtcg atcgtctcga tcatctcgag cgcggcgcga accgcggcgt cgcagtgccc
     1801 gggcagcggc agcggcgctc cgaaaattgc catcagtccg tcgcctgcca tgtgcgtcac
     1861 gatgccgccg tgccgtccga ttgcgtcgaa catcagcgtg tagtacgtgt tgaggagctc
     1921 gatcgtctcc tcgggaggct gcgattcggc gagcgacgtg aacccccgga tgtcggcgaa
     1981 catcacgctt gcgcgaatac gcttgccgcc gagcgcgaag ccgctctgct gcaggtcctg
     2041 cgcgacttcc ggcgtcgcga accggcgcac cagcgccttc tgctggtctc ggagacgctt
     2101 cttctcgagg ctcgcgccga cgcgggcctt gagcagcacc gcattgaccg gcttcgtcag
     2161 gtaatcctcg gcgccgagct cgatgcagcg cacgacgttg tcgatgcctt ccagcgacga
     2221 cgtgacgatc accggcacgt cgcgcagtgc caagtcgccg cggatctgct cgagaacctg
     2281 gaagccgtcc atctcgggca tctcgatgtc gagcagcacg agatcgaatg catcgcggcg
     2341 cagcgtgtcg agcgcgacgc gtccgttctc tgccgcgctc acgcgatggc ccatgaggtc
     2401 gaggttgcgc gtcaacagca gccggttgac cttgttgtcg tcgacgacga ggagccgcgc
     2461 gacgccgttc gcaaagtcag gcacgcgcaa gctccgcaag tgccttcgcc acgcgcgcgt
     2521 attcgaactc gagctcctcg agcggcgcgc cgttggcctc gcgcaccggc gccatgccgc
     2581 cgagctcgag gtccttcgcg agcttgccga gcgtcagcgc gccgaacgtg ttgctgttcg
     2641 acttgagcga gtgcgcggtg cgccgaaatg tgtcggcgtt gccggacgca tacgcgaggc
     2701 gaagctcgtc gagcatcagc ggcgcttcga cgaggaacgt gtcgacgagc tcgcacacga
     2761 attcggcacc cgccgtcgac tgcagctcac cgaatgcagc gaggtcgatc gtcggttggg
     2821 tcatgtcagt cgtgaagggc cgccccgaac gactgaccca ccccttgggg ggcagcgagc
     2881 ggagcgagcg tggggggatg tttcatcagt cgtgaagggc cgccccgaac gactgacccg
     2941 ccccttgggg ggcagcgagc ggagcgagcg tggggggatg tttcatcgca gcctattcct
     3001 cttctccaag tcgggcgaga tccgcgcgct gttctgccgt tccacgcgcc gccacgcgcg
     3061 caagcgcctc gaccagcgcg tccacgcgga tcggcttcgt catgtagtcg tccatgccgg
     3121 cggcgaggca gagctcccgg tcgccctgca tcgcattggc cgtcatcgcg acgatgcgcg
     3181 ggcgctcgct ggggcgaaag cgggcggtga tcgccttcga cgcctcgagc ccgtccatct
     3241 cgggcatctg cacgtccatc aacaccacgt cgtacgtctg tcgcgccacg ctctcgaccg
     3301 cctcgacgcc gttcgatgcg aggtcggcgc ggtagcccat ctgctgcaac aggcgcagcg
     3361 cgagcttctg attcaccacg ttgtcctcgg ccagcaggat gcgcagcgga tgccgcgcgg
     3421 ccatgccggc gtcgattctc ggcttcgcgg tcgcgaccgg ccgtggagaa gcatccgtca
     3481 tctcgtgggc gagcagtgtc atcagcgtgt cgaagagctg cgactggcgc agcggtttcg
     3541 cgagtgtcgc ggcgaagagc ccagcggcat cgcccgcctc gcggcggccg agcgaggtga
     3601 acagcacgag cggcagcatc gcgcgcatgc tgcgaatgcg cgtcgcgagc atgattccat
     3661 ccatttccgg catgtgcatg tcgaggatcg ccacatcgaa cgcatcgccg tcggcgatcc
     3721 attgcagcgc ctgcgccggg aactgcgtgt cgcgcgtcag catgccccat ttggcggcct
     3781 gcagcgcgag gatcctgcga ttggtcgcgt tgtcgtcgac gatcagcagg cgcttggccg
     3841 cgagcgcggg ctgcgtcccg atgaactcgc ggcgcgcgtt cggcgcgagc gcggccggct
     3901 ccgcgcggat cgtcacgaag aacgtggagc cggttccggg acccgcgctc tgtgcccaca
     3961 tcgtgccacc catcagctcc gccagccgct tgctgatcgc gagcccgagg ccggtgccgc
     4021 cgtatttgcg cgtcgtcgac gagtcggcct gcgaaaatga ccggaacagc cgcgcgagtc
     4081 cggcgtcggt gaggccgatg cccgtgtcgc gaatggcgaa gtgcagctcg tgtgcagcgt
     4141 cttgcattcc ggcgcttacg gtcagcacga cctcgcccgc ctcggtgaac ttcaccgcgt
     4201 tggacagcag gttgagcagg acctgtcgca gccgtgtcac gtcgccgttc acggcggcag
     4261 gaacgtcgcc ttcgaagacg taggcgatgt cgagatgctt ttccgcggcg cgcgtgctga
     4321 cgaggtcgag cgccgactcg acgcattccc gcaggtcgaa cgggtgcgcc tcgatgtcca
     4381 tccttcccgc ctcgatcttc gaaaagtcga ggatgtcgtt gatgatggtg agcagcgcgt
     4441 cgcccgaatc gcggatcgtc gatgcgtaat cgtgctgttc cgcgtcgagc ttcgtgtcga
     4501 gcaggagacc gctcatgccg atcaccgcat tcatcggcgt gcgaatctcg tggctcatgg
     4561 ttgcaaggaa cgcgctcttg gcttcgttcg cggtttcggc cgccgcgcgc gcatcgcgcg
     4621 cttcgttgaa caggcgcgcg ttctggattg cgatcgccgc ctggtccgaa aagattcgca
     4681 gcagctccac ctccttgtcg gtgtacggcc gcggcggctg gcggaacacg catatggcgc
     4741 caataccgcg gccttcccag aacatcgggc tgtataccga ggtgtagtgg ccgacggttt
     4801 ccacgagcct gtcgatcccg ggatagccca tcgcaagcgc ttcctgatcg gtggcccgga
     4861 ccgggcgtcg ctcgcggatt gcctgcccgg tgaacgtcgc atcgagcggc atcgatccga
     4921 tgtcccgcat cagcgtatcg aacgccgacc cgcgccacgc tgcgggatat acgcgaccgt
     4981 cctcgcgcac gagcatgacc gcgagctggt gcgcggtgaa cagacgctgg cagctgtcga
     5041 ggatcttgtc gaacaccggc gccgtgtccg acaccgagct gctgatgacc tggagcactt
     5101 cagcagtggc agtctgctgt tccagcgctt ccttcgtctc gttgaacagc cgcgtgttct
     5161 gaatcgcgat caccgcctgg tcgacgaacg actgcgccag cgcgatctcg ccgtcgctgt
     5221 aggcatgtgc cttggcgtgc gcgatcgcca gcacgccgac gcaatcgccg tcgcacacga
     5281 gcggcagcat cagcgacgaa tggaggctcg tgccctcgaa gaccgtccgc tcgtgcggcg
     5341 gcagctcgat cgccgaccag tccggaatat gcagcatcgc cttgctgacg aagacgcgcg
     5401 acgggaagtt cgccgcggga tcgatcggca ctcgcgccgg gtgttcgtcc agcctgcgcc
     5461 ggcgctgcgc ctcgtcgcgg ttcgctgtcg cgcctatccg gtagaaagcc ccatcgcggc
     5521 gcagcagcac ggcgaacgtc gctggcagca gcctgagcgc ggcctcgacg attgcgtcga
     5581 acacgggctg cacgtcggtc ggcgagccgc tgatgacgcg caagatgtcg gacgtcgccg
     5641 tctggcgatc gagcgcttcc tgcgtctgct tgaagagtcg cacgttctga attgcgatca
     5701 ccgcctggtc ggcgaacgtc tgcacgaggg cgatctccga cgcagaaaac ggacgcacct
     5761 ccctgcgcag catggcgatg acgccgatgg cctcgccgtc ctgaagcagc ggcacgttca
     5821 gcaccgtgcg aaatccgtgg cgcgcctgga gttcgcgcac gtccggatat tcggtctcga
     5881 tcagcggcaa cacgtcatgc aggtgaatgc atcgccgctc cagcaccgcg cgcccagcga
     5941 tcgacgtggt tcggatcggt agcgtttcgc cgcgcgagtc ggcgtcagcg ggtccatagc
     6001 tcgtcatcgc gcgcaaccga tcgccgtcga ccagccagac gcggcttccc tcggcgcggc
     6061 acagtcggcc ggcggcctcc gcgacagcgt ccagcaccgg ctgcacgtca gttggcgagc
     6121 ggctgatgac tttcaggatt tccgcggtcg ctgtctgctg atcgagtgcc tcttttgttt
     6181 cattgaacag ccgcgcgttc tgcaccgcga tcaccgcctg gtcggcgaac gtcttcagca
     6241 gcgcgagttc cttgtcggag aacgccttga ccggctggcg cagcaggcag atcgaaccaa
     6301 cgccgcgccc ctcccacaac atcggcaccc acaccgccga gaagttgccg atgagggttt
     6361 gcatttcgcg cagcgtagcg ggagcgtcct tcagcgctgc ggcgtcggct atgtgcacgg
     6421 gcgcgcgctc gcgcagcacc tgggcggtga tagtctgctc gagcggcttg gggaacgtgc
     6481 gaacgaccgc ctccatcgcc gcgccgtacc aggtgccggc gtgcagaagt ccgtcgtcgc
     6541 cgagcaggaa tacgccgacc tgttcgctgg cgaagagctg gccgcaactc tgcgcgatct
     6601 tgtcgaacac cggctgcgcg tcggccatcg agctgccaat cacctgcaat acctcggcgc
     6661 tggcagtctg ctgctccagc gcctcctgcg tctcgcggta gagctgcgcg ttcttgagcg
     6721 cgatcgccgc ctgccgcgac aatcccacga ggaactcgag ctcgtgcgcc tcgaacgggc
     6781 tgccgccgct gcgccacacc gccatcgcgc cttgcacagt ctcgccgccg agcagcggca
     6841 ccaccatcag ccgctcgtcc cggcgcatct cggtgcccgc aatctgcagc gcgcgcggat
     6901 cggccgccgt atcgttgatg agctccggcc ggccgctttc gatgagcgaa ccgatgatgc
     6961 cgtgcccggg ctcgaccacc gaggccctca gttggtcggc gagatcgccc agcgcgacca
     7021 gcgcgcggta gttccggcca tcggcgtcgg gcagaaaaat ggcgctgttg ccggcgctca
     7081 gcattgcctt ggcatggccg gcgatgcggt ccatcaccgt ggcgaggtcg agcgtcgacg
     7141 acaggtcgcg ccccacgtcc gacagcgccg acgattcgcg cgcgttgcgc tgtgtttccg
     7201 cgagcagccg cgcgttctcg agcgccacgc ccatcgacgc tgcgattgtc gatagcagat
     7261 gcacctgcgc gtcgtcgaac gcgtgctcgc gctcgaagct ttcgatccag atgacggcaa
     7321 ccaggcgttc gccgaccatc accgggacga agacgcacga caggctggtg tccgttcctt
     7381 ccagcgtcgt gacgccgatg gcatccatcg cggctcggtc gcccaacact accggccggc
     7441 cggttcgcaa tgcgcggttg atcggctttt cggcgtcgta cggccttggc ggcagcgtga
     7501 gccgcttgcc gtgctcgtag acgtacagcg ggcggacggt cgcggtcttg tagtcgttcc
     7561 aggcgatcgt catgtcgccg gtggcgaaca tccggcgcag ctggtcgccg acaacgtcaa
     7621 cgatggcctg gaagttgagc tcctttgcca tcccttgctg gatgctgttg atcacggcga
     7681 gctcggacga gcggcgttcg gtctccttca gcaggcgttg cgtctcgtcg aa
//
LOCUS       DASSVX010000283         3978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_795486, whole genome shotgun sequence.
ACCESSION   DASSVX010000283 DASSVX010000000
VERSION     DASSVX010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795486"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VFO53_12210"
     CDS             complement(<1..964)
                     /locus_tag="VFO53_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET8877085.1"
                     /translation="METAGLGPVLVFGLVAVALLFDFMNGLHDAANSIATVVSTRVLK
                     PRTAVMWAAFFNFIAFAVFGLHVANTIGTGIVDANVIDAHVIFGALTGAIAWNALTWA
                     LGLPSSSSHALIGGLAGAGVAKAGWGAVVFGGLGKTALGIVLSPLLGFALAQLIAVIV
                     LWANIRSTPFAVDRRFRVLQLISAGLYSLGHGGNDAQKTMGIIAVLLYSQGMLGGTFH
                     VPFWVVIACQLAMALGTLMGGWRIVHTMGSRITHLKPIQGFCAETGGAITLFLATGLG
                     VPVSTTHTITGGIVGVGWVNRPKSVRWRVAFRIIWAWLFTIQAAA"
     gene            complement(1058..1714)
                     /locus_tag="VFO53_12215"
     CDS             complement(1058..1714)
                     /locus_tag="VFO53_12215"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HET8877086.1"
                     /translation="MALNLFSRLMPPEKSFTSLFCEQAGCIVDAALELRDMVGPRQVG
                     IEAHIASIRTIETKSDAVVRQIFLSANRTFNAPIDREDILALAHELDDVVDLIEDTAK
                     GIQRYALTVFPEEMQTMVNAVVESAELLKKAVPFLDSITRDFREIYGICQRIGQIEEH
                     ADESFDAALSRIRSELREGKLDTIGYLDQKELYEWIERVVDKCDDVANQLQTITAKHV
                     "
     gene            1861..3075
                     /locus_tag="VFO53_12220"
     CDS             1861..3075
                     /locus_tag="VFO53_12220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8877087.1"
                     /translation="MSSVPFQQRGEWQHSVRANYDVAIVGGAIIGSAVAYFLKRLAPA
                     ADVAVIEPDRTYEFASTLRASGGARRQFSCPENLEMSNFSIPFIASAGETLAVDGEPA
                     HVEWHEGGYLFIVGPGDVDVLRRNCDAQHSHGVDARWLDAEALRARFPSMNVEGIAAG
                     VLTPEDGWCDPNGLLQGLRSKARALGAAYVDDRVVAIDHDAAAVRALRLASGATLCAD
                     VIVNAAGAWAQQVAAMVDMPLPIEPLRRFEHYFETPNAIEPLPYVKDTARLAFRPEGR
                     GYSGGLVDSAEPRGYNFDVDHDYFERAVWPALAQRFPAFEACRCRRTWSGLYEQNELD
                     GNPVIGNGSGRLANFHVVAGFSGHGMMHAPAAGRAIAELIVRGRFESIDLARLGYARI
                     ALHAPYPERGII"
     gene            3218..>3978
                     /locus_tag="VFO53_12225"
     CDS             3218..>3978
                     /locus_tag="VFO53_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HET8877088.1"
                     /translation="MKFRFPVVIIDEDYRSENASGLGIRALARAIEAEGTEVLGVTSY
                     GDLAGLAQQQSRASAFILSIDDEEFGAGSPDETRAVLANLRAFVEEIRFRNSEIPVFL
                     HGETRTSRHIPNDILRQLNGFIHMNEDTPEFVARYILREARTYLDSVAPPFFRALVHY
                     ANDGSYSWHCPGHSGGVAFLKSPAGQMFHQFFGENLLRADVCNSVDELGQLLDHSGPV
                     AASERNAARIFNTDELFFVTNGTSTSNKIVWHSAVG"
BASE COUNT          654 a         1379 c         1315 g          630 t
ORIGIN      
        1 acgccgccgc ctggatcgtg aacagccagg cccagatgat gcggaaggcg acgcgccagc
       61 gcacgctctt cggacggttg acccagccga cgccgacgat gccgccggtg atcgtgtgcg
      121 tggtcgacac cggcacgcct aatcccgtcg ccaggaacag cgtgatggcg ccaccggtct
      181 cggcacagaa gccctggatc ggtttgaggt gcgtgatccg cgagcccatc gtgtgcacga
      241 tgcgccagcc gcccatcagc gtgccgagcg ccatcgcgag ctgacacgca atcaccaccc
      301 agaacggcac gtggaacgtc ccgcccagca tcccctgcga atagagcagc accgcgatga
      361 tgcccatcgt cttctgcgcg tcgttgccgc catggccgag cgaatagagc ccggccgaga
      421 tgagctgcag cacgcgaaag cgccggtcga cggcgaacgg cgtcgagcgg atgttcgccc
      481 acagcacgat gacggcgatg agctgcgcga gcgcgaagcc cagcagcggc gacagcacga
      541 tgccgagcgc cgtcttgccg agcccgccga acacgaccgc gccccatccc gctttcgcga
      601 cgccggcccc cgcgaggccg ccgatcagcg cgtggctgct cgacgacgga agcccgagcg
      661 cccaggtgag cgcattccac gcgatggcgc ccgtcagcgc gccgaagatc acgtgcgcgt
      721 cgatcacgtt cgcatcgacg atgcccgtgc cgatcgtgtt cgcgacgtgc agcccgaaga
      781 ccgcaaaggc gatgaagttg aagaacgccg cccacatgac cgccgtgcgc ggcttcagca
      841 cgcgcgtcga gacgacggtg gcgatcgagt ttgccgcgtc gtgcagcccg ttcatgaagt
      901 cgaacagcag cgcgacggcg accaggccga acacgaggac ggggccgagc ccggcagtct
      961 ccatcaacgg cgtgtcggtg gcgccgggcc gccccaagcc actgacggcg ccccctttgg
     1021 ggacagcggg cgtggcgagc gtgggggtcg tttcatccta gacgtgcttc gcggtgatcg
     1081 tctgcagctg gttcgcgacg tcgtcgcact tgtcgacgac gcgctcgatc cactcgtaca
     1141 gctccttctg gtcgagatag ccgatcgtgt ccagcttgcc ttcgcgaagc tcgcttcgaa
     1201 tgcgcgacag cgccgcgtcg aacgactcgt cggcgtgctc ctcgatctgg ccgatgcgct
     1261 ggcagatgcc gtagatctcg cgaaagtcgc gcgtgatcga gtcgaggaac ggaaccgcct
     1321 tcttgaggag ctcggcgctt tcgaccaccg cgttcaccat cgtctgcatt tcctcgggaa
     1381 acaccgtcaa tgcgtagcgc tggatgccct tcgcggtatc ctcgatcagg tcgacgacat
     1441 cgtcgagctc gtgcgcgagc gccaggatgt cctcgcggtc gatcggcgcg ttgaacgtgc
     1501 gattggcgga caggaatatc tggcgcacga ccgcgtcgga cttcgtctcg atcgtgcgga
     1561 tcgacgcgat gtgcgcctcg atgcccacct ggcgcgggcc gaccatgtcg cgcaactcca
     1621 gggcggcgtc gacgatgcaa ccggcctgct cgcagaaaag gctggtgaag ctcttttcgg
     1681 gcggcatcag ccggctgaac agattgaggg ccatgggaaa gggtgcgcag tggatacgag
     1741 tcgcggccgt cgccggacct cgcgcgccgg ctgcgcggcg gtgcggcggg cggcgggtag
     1801 acaggcacga tggtacccga caatgatgaa cgcattgcac gatcacctcc ccggacagcc
     1861 atgagcagcg tcccttttca gcaacgcggc gaatggcagc actcggtgcg agcgaactac
     1921 gacgtcgcaa tcgtcggcgg cgcgatcatc ggctcggcgg ttgcctactt cctgaagcgg
     1981 ctggcgccgg ccgccgatgt cgccgtgatc gagcccgacc gcacctacga attcgcctcg
     2041 acgctgcgcg cgtccggggg tgcgcgccgg cagttctcgt gccccgagaa cctcgagatg
     2101 tcgaacttca gcatcccgtt catcgcctcg gccggcgaaa cgctcgcggt cgacggggag
     2161 cctgcgcatg tcgaatggca cgagggcggc tacctcttca tcgtcggccc gggcgacgtc
     2221 gacgtgctgc gccgcaattg cgacgcgcag cattcgcacg gcgtcgacgc ccgttggctc
     2281 gacgccgagg cgctgcgcgc gcgctttccg tcgatgaacg tcgagggcat cgccgccggc
     2341 gtgctgacgc ccgaggacgg ctggtgcgat cccaacggct tgctgcaggg gttgcgcagc
     2401 aaggcgcgcg cgctcggtgc agcgtacgtc gacgatcgtg tcgtcgccat cgaccacgat
     2461 gccgccgcgg tgcgggcgct gcggcttgcg tcaggcgcca cgctgtgcgc cgatgtcatc
     2521 gtgaacgcgg cgggtgcgtg ggcgcagcag gtcgcggcga tggtggacat gccgctgccg
     2581 atcgagccgc tgcgccgctt cgagcactat ttcgaaacgc cgaatgcgat cgagccgctg
     2641 ccctacgtga aggacacggc acggctcgcc tttcgcccgg aaggccgcgg ctattccggc
     2701 gggctcgtcg attcggccga gccgcgcggc tacaacttcg atgtcgacca cgactacttc
     2761 gagcgcgccg tctggccggc actcgcgcaa cgttttccgg cgttcgaggc gtgccgctgc
     2821 cggcgcacct ggtcggggct ctacgagcag aacgagctcg acggcaaccc ggtgatcggc
     2881 aacggcagcg ggcgcctcgc caatttccac gtcgtcgcgg gcttttccgg ccatggcatg
     2941 atgcacgcgc ccgcggcggg gcgcgcgatc gccgagctga tcgtccgcgg gcgcttcgaa
     3001 tccatcgacc tcgcgcggct cggttacgcg cgcatcgctc tgcacgcgcc gtatcccgag
     3061 cggggaatta tctgaacggc atcccggctc gcgccgcgtg gcgcacaatg cgcgccggat
     3121 aaacgacgga cagcgagccg ccccccggga actatcggcc ccgcgccagt ctctctacac
     3181 gcttcgcgcc gcgctgccgg cgccatcgga gaacgcaatg aaattccgct ttccggtagt
     3241 catcatcgac gaggactacc gttcggaaaa cgccagcggc ctcggcattc gcgcgctcgc
     3301 ccgcgccatc gaggccgagg gcaccgaagt gctgggcgtg acgagctacg gcgaccttgc
     3361 cggtctcgcg cagcagcagt cgcgcgcctc cgcattcatc ctgtcgatcg acgacgagga
     3421 gttcggcgcg ggctcgcccg acgagacgcg cgcggtgctc gcgaacctcc gcgcgttcgt
     3481 cgaggagatc cgcttccgca actccgaaat cccggtcttc ctgcacggcg agacgcgcac
     3541 gtcgcgccac atcccgaacg acatcctgcg ccagctgaac ggcttcatcc acatgaacga
     3601 ggacacgccc gagttcgtgg cgcgctacat cctgcgcgaa gcgcgcacgt atctcgacag
     3661 cgtcgcgccg ccgttcttcc gcgcgctcgt ccactacgcg aacgacggct cgtactcgtg
     3721 gcactgcccg ggccattcag ggggcgtcgc cttcctgaag agcccggcag gacagatgtt
     3781 tcatcagttc ttcggcgaga acctgttgcg cgccgacgtg tgcaattcgg tcgacgagct
     3841 cggccagctc ctcgaccatt cggggccggt tgccgcgtcc gagcgcaacg ccgcgcgcat
     3901 cttcaacacc gacgagctgt tcttcgtcac caacggcacg tcgacgtcga acaagatcgt
     3961 ctggcacagc gccgtcgg
//
LOCUS       DASSVX010000284         4621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_379131, whole genome shotgun sequence.
ACCESSION   DASSVX010000284 DASSVX010000000
VERSION     DASSVX010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379131"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /gene="lipB"
                     /locus_tag="VFO53_12230"
     CDS             complement(<1..628)
                     /gene="lipB"
                     /locus_tag="VFO53_12230"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET8877089.1"
                     /translation="MQTDRAPDFLTRRAALPVATIRPLGHTGYEATWRAMQAFTDARS
                     AETGDEIWLTEHEPVYTLGLAGRREHLLRGNGIPVIKVDRGGQITYHGPGQLVAYLLF
                     DLRRARLGVREMVRRIDGAVITWLATLGIRAYGKTSAPGVYVEHVGVEAKIAALGLRV
                     RNGCTYHGVAVNIDMDLAPFADIDPCGYPGLAVTQLADFGVRTTVDDAG"
     gene            complement(651..941)
                     /locus_tag="VFO53_12235"
     CDS             complement(651..941)
                     /locus_tag="VFO53_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HET8877090.1"
                     /translation="MDSPIGVPIVAQVSALEFPCTFPIKIMGRTRDGFAQAIADIVCR
                     HAPDFDPGALEMRTSSAGNYLSMTATINATSREQLDDLYRELVAHPMVAMVL"
     gene            complement(1017..2237)
                     /locus_tag="VFO53_12240"
     CDS             complement(1017..2237)
                     /locus_tag="VFO53_12240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HET8877091.1"
                     /translation="MPDTLQKVIANRYAGRDLPVALVLPDGARVPLSSKPEVDVIARS
                     LAGLRALAAPALGRLARAYVRGDVDFTGSARRVLAIAERMVGDIAHGQESARAKLLTF
                     AHHRRSNRSNISHHYDVSNAFYRMWLDARMVYSCAYFRKPDDTLDDAQAQKIDHICRK
                     LRLAKGERFLDIGCGWGALILWAAENYGVHATGITLSRNQFEHVRREIVARGLSDRVR
                     VELRDYADLPDQEAFEKIASVGMFEHVGPRNYGRYFGKIRRLLVPGGFVLNHGITHNQ
                     LTGRALGSGIGEFVEEYVFPGGELAHVSRVTEGLAAQGLEMVDGEALREHYAKTLWHW
                     VDRLEAHADAARAEVGEEKYRVWRIYMAGSAHAFDRGWLSLWQLLAGKPCADGRLPHP
                     LTREYMYARSNGTA"
     gene            2353..2877
                     /locus_tag="VFO53_12245"
     CDS             2353..2877
                     /locus_tag="VFO53_12245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET8877092.1"
                     /translation="MSGKGRHAGTGTTAFNERQLRDALAQFATGVTIVCARAPDARYV
                     GFTANSFSSASLDPPLILWTLSQRSTSLAAFDASPRYSVNVLAAGQVELARRFSRPHA
                     DRFANVPYRLGWSDAPLIDGCIAWFECRHHARHHAGDHVLFIGEVMTVERAPGQGLVF
                     QHGQFGGTPPPADK"
     gene            complement(2961..3806)
                     /locus_tag="VFO53_12250"
     CDS             complement(2961..3806)
                     /locus_tag="VFO53_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HET8877093.1"
                     /translation="MIDADRVVYLNGEFLPLRDAKISVLDRGFIYGDGVYEVVPVYAR
                     KPFRMPQHLARLTHSLDGIRLADPHRDRWAALIGELIGRQPFDNQAVYLQVTRGVAKR
                     DHAFPQGVTSTVFMMSNPLALPTREQVEHGVAVITAEDNRWLRCDLKTISLVGNVLMR
                     ELAADHDAIETVMFRNGCLTEASASNVLIVHEGTIIAPPKDNLILPGITYDATLEIAN
                     AIGVPLEVRAVTRAEALGADEMWLSSSSKEVLAITRVDGQAFAGGAPGPVFRRVWEAF
                     QARKR"
     gene            complement(3803..>4621)
                     /locus_tag="VFO53_12255"
     CDS             complement(3803..>4621)
                     /locus_tag="VFO53_12255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET8877094.1"
                     /translation="LHGVIIQSGNDASIAIAELVGGTEEGFVDRMNQEAAKLGMTGTH
                     FTNADGLPDPQHYSTAADLAKLTVALVRDFPEYYPLYAQKEYRYNNITQSNRNRLLWT
                     DPYVDGVKTGHTDAAGFCLVASAKRGDRRLVSVVLGAGSDSSRAAESQKLLNWGFQSF
                     DTVALYQSGKPVTTLRVFKGAKHDVTAGFLADRYLTLPKGKADKLALTLESKDPLVAP
                     VLSGQAVGIVKVALEGKPVAEFPLIALEQVPLANLFGRAVDTVRLWFTSHQGKS"
BASE COUNT          762 a         1545 c         1543 g          771 t
ORIGIN      
        1 gacccgcgtc gtccaccgtg gttcgcacgc cgaaatcggc gagctgcgtc accgcgagtc
       61 ccgggtagcc gcagggatcg atgtcggcga agggagcgag gtccatgtcg atgttgacgg
      121 cgacgccgtg gtacgtgcag ccgttgcgca cgcgcaatcc gagcgcggcg attttcgcct
      181 cgacgccgac gtgttcgacg taaacgccgg gcgccgatgt cttgccgtaa gcgcggatgc
      241 caagggtcgc aagccatgtt atcaccgcgc cgtcgatgcg ccggaccatc tcgcgcacgc
      301 cgagcctcgc gcgccgaaga tcgaacagca ggtaggcgac gagctggccc ggaccgtgat
      361 aggtgatctg accgccgcgg tcgaccttga tcaccgggat gccattgccg cgcaacagat
      421 gctcgcgccg tcccgcgagt ccgagcgtgt agaccggttc gtgctcggtg agccagatct
      481 cgtcgccggt ttccgcggag cgcgcgtcgg tgaatgcctg catcgcgcgc caggtggctt
      541 cgtagccggt gtggccgagc gggcggatcg tcgcgacagg caacgccgcg cgccgcgtca
      601 ggaaatcggg cgcgcggtcc gtctgcatgc gccgagcgcc accgccgggt tcacagcacc
      661 atcgccacca tcgggtgtgc gaccagctcg cggtagagat cgtcgagctg ctcgcgcgac
      721 gtcgcgttga tcgtcgcggt catcgacagg taattgccgg cgctcgacgt gcgcatctcg
      781 agcgcgcccg ggtcgaaatc gggcgcatgc cggcacacga tgtcggcgat ggcctgcgcg
      841 aagccatcgc gggtgcggcc catgatcttg atcgggaacg tgcagggaaa ttcgagcgca
      901 ctcacctgcg cgacgatggg aactccgatt ggcgaatcca tttgcggctc cgatagggcg
      961 cattgaaggc ccgacttgcg aatatgcggc cgccttcgcc caacacaacg gccgcgttac
     1021 gccgtgccgt tcgagcgcgc gtacatgtac tcccgcgtca gcggatgcgg cagccggcca
     1081 tcggcacacg gcttgccggc cagcaactgc cacagcgaaa gccagccgcg gtcgaatgcg
     1141 tgcgccgacc ccgccatgta gatccgccac acccggtatt tctcctcgcc cacctccgcg
     1201 cgcgccgcgt ccgcatgcgc ctcgagccgg tccacccagt gccatagcgt cttcgcgtaa
     1261 tgctcgcgca gcgcctcgcc gtcgaccatc tcgaggccct gcgcggcgag accttccgtg
     1321 acgcgcgaca cgtgggcgag ctcgcccccc gggaacacgt attcctcgac gaactcgccg
     1381 atgccgctgc cgagcgcgcg gccggtgagc tggttgtgcg tgatgccatg gttcagcacg
     1441 aagccgccgg ggacgaggag gcggcggatc ttgccgaaat agcggccgta gttgcgcggc
     1501 ccgacgtgct cgaacatgcc gacgctcgcg atcttctcga acgcttcctg gtcgggcaaa
     1561 tcggcgtaat cgcgcaattc gacgcggacg cgatcggaaa gcccgcgcgc gacgatctcg
     1621 cgccgcacgt gctcgaactg gttgcgcgac agcgtgatgc ccgttgcgtg gacgccatag
     1681 ttctcggccg cccagaggat cagcgcgccc cagccgcagc cgatgtcgag gaagcgctca
     1741 cccttcgcaa ggcgcagctt gcggcagata tggtcgatct tctgcgcctg ggcatcgtcg
     1801 agcgtgtcgt ccggtttcct gaaataggcg cacgaataga ccatgcgcgc atcgagccac
     1861 atgcggtaga acgcgttcga cacgtcgtag tggtgcgaga tgttgctgcg attgctgcgt
     1921 cggtggtgcg cgaacgtcaa cagcttcgcg cgggcgcttt cctggccgtg cgcgatgtcg
     1981 ccgaccatcc gctcggcgat ggcgaggacg cgccgcgcac tcccggtgaa gtcgacgtcg
     2041 ccgcgtacgt acgcgcgtgc aagccgcccc agcgccggcg cggcaagcgc ccgcagcccg
     2101 gcgagcgagc gggcgatgac gtcgacttcg ggcttcgacg atagcggcac gcgggcgccg
     2161 tcgggaagca cgagcgccac gggcaggtcg cgcccggcat agcggttcgc gatcactttc
     2221 tgcaaggtgt ccggcatctt gttgggggtg cggattcacg cgaggacaca aagtgtagca
     2281 agcgggcgaa actgcatccc tccaaaggac tgcgtcgaac ggcaccgcag ggccttgcgg
     2341 tacattgccg gcatgtcagg caagggccgc cacgccggaa ccggcacgac agccttcaac
     2401 gagcggcagc tgcgcgatgc gctggcacag ttcgcgaccg gcgtcacgat cgtctgcgcc
     2461 cgcgcgcccg acgcccgcta cgtgggattc accgccaact cgttcagttc cgcgtcgctc
     2521 gatccgccgc tgatcctgtg gacgctgtcg cagcgctcga cgagcctcgc cgcgttcgac
     2581 gcgagcccgc gctactcggt caacgtactc gcggcggggc aggtggagct cgcgcggcgc
     2641 ttctcgcgac cgcatgctga ccgcttcgcg aacgtcccct atcggctggg gtggtcggat
     2701 gcgccgctga tcgacgggtg catcgcctgg ttcgaatgcc gccaccacgc gcgacaccac
     2761 gccggggatc acgtgctgtt catcggcgag gtgatgaccg tggagcgcgc acccggacag
     2821 ggtctcgtgt tccagcatgg ccagttcggc ggaacgccgc cgcctgccga taaatagggt
     2881 cagagacgac tttcgccgac catcggcccc actgccgcgc tcatgcgccg aaagtcgtct
     2941 ctgaccctat ttcggtgcga ctatcgtttt cgcgcctgaa atgcttccca cacacgccgg
     3001 aagacgggac ccggcgcgcc gccggcgaac gcctggccgt cgacgcgcgt gatcgcgagc
     3061 acttccttcg acgacgacga cagccacatt tcgtcggcgc cgagcgcttc ggcgcgcgtc
     3121 acggcgcgca cctcgagcgg aacgccgatc gcgttcgcga tttcgagcgt cgcatcgtaa
     3181 gtgatgcctg gcaggatcag gttgtccttc ggcggagcga tgatcgtgcc ctcgtgcacg
     3241 atcagcacgt tcgacgccga cgcctcggtg aggcagccgt tgcggaacat caccgtctcg
     3301 atcgcgtcgt ggtcggcggc aagctcgcgc atcagcacgt tgccgacgag cgaaatcgtc
     3361 ttgaggtcgc agcgcagcca ccggttgtcc tcggcggtaa tcacggcaac gccgtgctcg
     3421 acctgctcgc gcgttggcag cgcgagcgga ttgctcatca tgaacaccgt cgacgtgacg
     3481 ccttgcggaa acgcgtggtc gcgcttggca acgccgcgcg tgacctgcag gtagacggcc
     3541 tggttgtcga acggctggcg cccgatcagc tcgccaatca gcgccgccca ccgatcgcga
     3601 tgcgggtcgg cgaggcgaat gccgtcgagg ctgtgcgtca gccgcgcaag gtgttgcggc
     3661 atccggaagg gcttccgcgc gtacaccggc accacttcgt agacgccgtc gccgtaaatg
     3721 aagccgcggt cgagcacgga gattttcgcg tcgcgaagcg ggaggaattc tccgttcagg
     3781 tagacgacac ggtcggcgtc gatcatgatt tcccctggtg gctggtgaac cagagccgaa
     3841 ccgtatcgac ggcgcggccg aagagattgg caagcggaac ctgctcgagc gcgatcagcg
     3901 gaaactcggc aaccggcttg ccctcgagcg ccaccttgac gattccgacg gcctggccgc
     3961 tcagcaccgg tgcgacgagc gggtccttcg attcgagcgt cagcgcgagc ttgtccgcct
     4021 tccccttggg cagcgtgagg tagcgatcgg cgaggaaccc ggcggtgacg tcgtgcttcg
     4081 cacccttgaa cacgcgcagc gtggtcaccg gcttgcccga ctggtaaagc gccaccgtat
     4141 cgaacgactg gaagccccag ttgagcagtt tctggctctc cgcggcacgc gacgaatcgg
     4201 agcctgcacc gaggacgacc gacacgaggc ggcggtcgcc gcgcttggcg gatgcgacga
     4261 ggcagaaccc ggccgcatcg gtgtggcccg tcttgacgcc gtcgacgtac ggatcggtcc
     4321 acaacagccg gttgcgattc gattgcgtga tgttgttgta gcgatattcc ttctgcgcat
     4381 acagcgggta gtactcggga aaatcacgca ccagcgcgac ggtcagcttc gcgaggtcgg
     4441 ccgccgtcga gtaatgctgc gggtcgggga ggccgtccgc attggtgaaa tgcgttcccg
     4501 tcatgccgag cttcgccgct tcctggttca tcctgtcgac gaagccttcc tcggtaccgc
     4561 caacgagctc ggcaatcgca atcgacgcat cgttgcccga ctggatgatg acgccgtgca
     4621 g
//
LOCUS       DASSVX010000285         3736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_595862, whole genome shotgun sequence.
ACCESSION   DASSVX010000285 DASSVX010000000
VERSION     DASSVX010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595862"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..481)
                     /gene="rpmA"
                     /locus_tag="VFO53_12260"
     CDS             complement(215..481)
                     /gene="rpmA"
                     /locus_tag="VFO53_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET8877095.1"
                     /translation="MAHKKAGGSSRNGRDSESKRLGVKVYGGEAIRAGGIIVRQRGTR
                     IHPGRNVGLGKDHTLYALVDGKVNFGVRGERQHKYANVEPQEAA"
     gene            complement(495..806)
                     /gene="rplU"
                     /locus_tag="VFO53_12265"
     CDS             complement(495..806)
                     /gene="rplU"
                     /locus_tag="VFO53_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET8877096.1"
                     /translation="MYAVVKTGGKQYKVSAGEKLKVEQIPADVGAEVVLDQVLMVGGG
                     DNVRVGQPTVAGATVTATVVAHGRGEKVRIFKMRRRKHYQKHQGHRQNYTELRIEAIV
                     G"
     gene            977..1945
                     /locus_tag="VFO53_12270"
     CDS             977..1945
                     /locus_tag="VFO53_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET8877097.1"
                     /translation="MSLDFLNPAIGDDMAAVDRVLRESLRSDVVLIRQVANYIIAGGG
                     KRLRPALLLLAARACGYAGRDHHTLAAAIEMIHTATLLHDDVVDESQLRRGHATANAE
                     FGNPASVLVGDFLYSRTFQLMVSVDRMRVLSIIADATNLIAEGEVLQLMHAGDAALDE
                     RAYFAVIERKTAKLFEAAGRLGAVLGDANAQCEDALARYGTHLGIAFQIMDDVLDYSG
                     AEAEIGKNLGDDLAEGKMTLPLIRALAVGTRAEAEVIRHAVAGGGLTDFAPVVAALHR
                     TGALSYAQQLAQAQAEAAGACLAVLPASPAASGLLQLSTFAARRSY"
     gene            1984..2060
                     /locus_tag="VFO53_12275"
     tRNA            1984..2060
                     /locus_tag="VFO53_12275"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2018..2020,aa:Pro,seq:cgg)
     gene            2060..3109
                     /locus_tag="VFO53_12280"
     CDS             2060..3109
                     /locus_tag="VFO53_12280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8877098.1"
                     /translation="MIEPLKRALKRLPPLGRLIAARDALVAERDALLESQRSLAGERE
                     ALLLQRDTLRGCHAALTSERDMLLQWAGPEPRYVPPGHYYSPIASIADLRANSARLFA
                     PFPRELPGIDLNEPGQLALLSLLEGYYRDLPFGADPIGQLRYGLENPGYAWSDGIFLY
                     AMIRHLAPKRIVEVGSGNSSCLMLDVNDLHFGGRLRCTFVEPEPARFLALIRDTDRTA
                     IDIIAKPLQDVPLSHFTTLDANDILFVDSSHVSKAGSDVNHLFAEILPRLRSGVHVHF
                     HDVFFPFEYPPEWLYQGKAFNEAYVLRAFLAYNAAFEIVLFNTFLEHFHAQRFAERMP
                     LCLRNVGGSLWLRRR"
     gene            complement(3131..>3736)
                     /locus_tag="VFO53_12285"
     CDS             complement(3131..>3736)
                     /locus_tag="VFO53_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8877099.1"
                     /translation="PFFTTKDVGKGSGLGLSQVYGFIKQSDGHAKIYSEPGDGTTIKL
                     YLPRLVTAGDAVDTSADVSTLPAGNTGRVVLVVEDDEDVRAHAVAILSELGYSVLEAA
                     EGAVALRVLDAQPDIGMLFTDVGLPGGLNGRQLADAARSRYPELLVLFTTGYARNAIV
                     HQGRLDPGVELITKPFTFAALANKVNAIFEQHERPPRAPAL"
BASE COUNT          570 a         1275 c         1185 g          706 t
ORIGIN      
        1 cgttcttcct gctcggcagg ctcttctcga acctcggggt gctgaacgat tggccgccgc
       61 tcttctcagc ggtgtttccg cttgccttct tcaccgcgat cgcgctcggg atgctgcact
      121 tcatcgagcg gcgatagaaa cgaaaagggg ccagggtcga ctttccctgc ggaaaagtcg
      181 accctggccc cttttcggtt gaccgtgctt cgggttacgc cgcttcctgc ggctcgacgt
      241 tcgcgtactt gtgctgccgc tcgccgcgca cgccgaagtt caccttgccg tcgacgagcg
      301 cgtacagcgt gtgatccttg ccgaggccga cgttgcggcc cgggtgaatg cgcgtaccgc
      361 gctggcgaac gatgatgccg ccggcgcgaa tcgcttcgcc gccatagacc ttgacgccaa
      421 ggcgcttcga ttcggaatcg cggccgttcc tcgaactgcc gcctgctttc ttgtgtgcca
      481 tccgtgtctc tccttcagcc gacgatcgct tcgatgcgaa gctcggtgta gttctggcga
      541 tgcccctggt gcttctggta atgcttgcgc cggcgcatct tgaagatccg gaccttttcg
      601 ccgcgcccgt gcgcaacgac ggtggccgtg accgtcgcgc ctgcgacggt aggctggccg
      661 acgcgaacgt tgtcgccccc gccaaccatc agcacctggt ccaggaccac ttccgcgccc
      721 acgtccgccg gtatctgttc taccttgagt ttttcgccgg cggaaacctt gtattgcttg
      781 ccgccggttt ttacgaccgc gtacatgagt cgaactcccg tcaatgggaa aagccttgga
      841 ttatagcgcg attcgcccgc gaagtgaatc cgccccgtct ctccggtaga atggccgctc
      901 cctgcggggc gttgcgcgag cgacgtgcct tcacgcgcgg tcgaatgttt cgccaacgct
      961 gctgttgacg ccctttgtga gcctcgactt tctcaatccc gccatcggcg acgacatggc
     1021 cgcggtcgac cgcgttctgc gcgaaagcct gcgctccgac gtcgtgctca tccgccaggt
     1081 cgccaattac atcatcgccg gcggtggcaa gcggctgcgc ccggcgctgc tgctgctcgc
     1141 tgcgcgcgca tgcggctacg ccggccgcga ccaccatacg ctcgccgcgg ccatcgaaat
     1201 gatccacacg gcgacgctgc tgcacgacga cgtcgtcgac gagtcgcagc tgcggcgcgg
     1261 gcacgcgacg gcgaacgccg aattcggcaa tcccgcctcc gtcctcgtcg gcgacttcct
     1321 ctattcgcgc acgttccagc tgatggtcag cgtcgaccgg atgcgcgtgc tctcgatcat
     1381 cgccgatgcg acgaacctga tcgccgaagg cgaagtgctg cagttgatgc acgccggcga
     1441 cgccgcgctc gacgagcgcg cgtacttcgc ggtgatcgag cgcaagacgg cgaagctgtt
     1501 cgaagccgcg gggcgtctcg gtgccgtcct cggcgacgcg aatgcgcaat gcgaggacgc
     1561 gctcgcgcgc tacgggacgc acctcggcat cgcattccag atcatggacg atgttctcga
     1621 ctattccggt gcggaggccg agatcggcaa gaacctcggc gacgatctcg ccgaaggcaa
     1681 gatgacgctg ccgctgatcc gtgcgctcgc ggtgggaacg cgcgccgagg cggaggtgat
     1741 ccgtcatgcg gtcgcgggcg gcggattgac cgatttcgcc ccggtggtgg cggcgttgca
     1801 tcgaaccggc gcactgtcgt atgcgcagca gcttgcacag gcgcaagccg aggcggcggg
     1861 cgcatgtctt gccgtgctgc cggcgtcgcc tgccgcgtcg ggtctgctac aattatcgac
     1921 tttcgctgcg cggcgcagtt actgatttcc cgggccccgc gaagcgcaaa ccggtgtcgg
     1981 aaacggggtg tagcttagcc tggtagagcg ctacgttcgg gacgtagagg ccggaggttc
     2041 gaatcctctc accccgacca tgatcgaacc cctgaagcgc gcgctgaagc ggctcccacc
     2101 gctcggtcgc ctgatcgcag cgcgcgatgc gctcgtcgcc gagcgcgacg ccctgctcga
     2161 atcgcaacgc agcctcgccg gtgaacgcga ggcgctgctg ctgcagcgcg acacgctgcg
     2221 cggatgccat gcggcactga cgagcgagcg cgacatgctg ctgcagtggg cgggtcccga
     2281 gccgcgttac gtgcctccgg gccactatta ctcgccgatt gcgtcgatcg ccgatcttcg
     2341 cgcgaactcg gcgcgcctgt ttgcgccgtt cccgcgcgag cttccgggca tcgacctcaa
     2401 cgagcccggc cagcttgcgc tgctgtcgct gctcgaaggc tactatcgcg acctgccgtt
     2461 cggtgccgat ccgatcggac agctccgcta cgggctcgaa aatcccggct acgcgtggtc
     2521 ggacggaata tttctctacg cgatgatccg ccatctcgcg ccgaagcgga tcgtcgaggt
     2581 cggctccggc aattcgtcgt gcctgatgct cgacgtgaac gacctgcact tcggcggccg
     2641 gctgcgatgc acgttcgtcg agcccgaacc ggcgcgcttc ctcgcgctga tccgcgacac
     2701 cgaccgcacc gcgatcgaca tcatcgcgaa gccgctgcag gacgtgccgc tgtcgcactt
     2761 cacgacgctc gacgccaacg acatcctgtt cgtcgattcg tcgcacgtca gcaaggccgg
     2821 aagcgacgtc aatcaccttt tcgccgagat ccttccgcgg cttcgatccg gcgttcacgt
     2881 ccacttccac gacgtgttct ttccgttcga gtatccgccc gaatggctct accaggggaa
     2941 ggcgttcaac gaggcatacg tcttgcgcgc gttcctggcg tacaacgccg cattcgagat
     3001 agtgctgttc aacacgttcc tcgagcactt tcacgcgcag cgttttgccg agcgcatgcc
     3061 gctgtgcctg cgcaacgttg gcggcagcct ctggctgcga cggcgttgaa tcggtccgcg
     3121 ccgaaaaggc tcaaagcgcc ggcgcacgcg gcggccgctc gtgctgctcg aaaatcgcgt
     3181 tcaccttgtt cgcgagcgcg gcgaacgtga acggcttcgt gatgagctcg acgccgggat
     3241 cgagacggcc ctggtggacg attgcgttgc gcgcataacc ggtggtgaac agcacgagca
     3301 gctcgggata gcggctgcgc gccgcgtcgg cgagctggcg cccgttgagc ccgcccggca
     3361 gcccgacatc cgtgaacagc atgccgatgt cgggttgcgc gtcgagcacg cgtagcgcca
     3421 ccgcgccctc cgcggcttcg agcaccgagt agccgagctc gctcaggatc gcgaccgcgt
     3481 gcgcgcgcac gtcctcgtcg tcctcgacga cgagcacgac gcggccggtg ttccctgcgg
     3541 gcagcgtcga aacgtccgcg gacgtgtcga ccgcatcccc ggccgtgacg agccgcggca
     3601 ggtagagctt gatcgtggtc ccgtcaccgg gttcgctgta gatcttggcg tgtccgtccg
     3661 actgcttgat gaagccgtac acctgcgaca gcccgagacc cgagcccttg ccgacgtcct
     3721 tcgtggtgaa gaacgg
//
LOCUS       DASSVX010000286         4191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1079108, whole genome shotgun sequence.
ACCESSION   DASSVX010000286 DASSVX010000000
VERSION     DASSVX010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079108"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..643
                     /gene="accB"
                     /locus_tag="VFO53_12290"
     CDS             194..643
                     /gene="accB"
                     /locus_tag="VFO53_12290"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET8877100.1"
                     /translation="MDLRKLKTLIDLVETSGIAELEIQEGEERVRITRAVVQTPQPLM
                     FHPQHQLAMPAPVAIAPVPEAPVAPVDEGHTVKSPMVGTFYRCASPGAAPFVEVGDTV
                     AQGDTLCIVEAMKLMNEIEADASGTIKAILVENGQPVEFGQPLFVIG"
     gene            703..2058
                     /gene="accC"
                     /locus_tag="VFO53_12295"
     CDS             703..2058
                     /gene="accC"
                     /locus_tag="VFO53_12295"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET8877101.1"
                     /translation="MFDKVLIANRGEIALRIQRACRELGIKTVVVHSEADRDAKYVAL
                     ADESVCIGPAPSAQSYLNIPAIIAAAEVTDAQAIHPGYGFLSENADFAEKVERSGFVF
                     IGPRPDTIRLMGDKVSAKVAMTKAGVPCVPGSEGALPEDPKEIVRIARGVGYPVIIKA
                     AGGGGGRGMRVVHTEAALLPAVALTRTEAHAAFGNATVYLERFLGNPRHIEIQVLADE
                     FKNALCLFERDCSMQRRHQKIIEEAPAPGVNARALARITERAADACRKLGYRGAGTFE
                     FLYQDEEFFFIEMNTRVQVEHPVTEMITGIDIVQEQLRIAAGQKLRFRQRDIQRRGHA
                     IECRINAEDPWTFVPSPGRITAWHAPGGPGIRVDSHAYQNYFVPPNYDSMIGKVIAYG
                     ETREQAIARMRVALSEMVVGGIRTNIPLHQELLLDEKFLRGGTSIHYLEERLAKRKQA
                     A"
     gene            2055..2951
                     /gene="prmA"
                     /locus_tag="VFO53_12300"
     CDS             2055..2951
                     /gene="prmA"
                     /locus_tag="VFO53_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET8877102.1"
                     /translation="MSYLAVRFDVEAAQADRWSDALLEAGALSVDAADPRAGTLDESA
                     IFGEPADAAPGWWPITRLTALCANECDPALLVRTAARCVEQALPVFETHALPDQDWVR
                     ATQAQFEPIRINDNLWIVPTWRDPPRPGAVNIRLDPGLAFGTGAHPTTRLCLAWLAEQ
                     VARTDSVLDYGCGSGVLAIAAARLGAACVSGVDIDAQAVRASVDNALANGVHGQFRQV
                     DALAPQTFSIVVANILANPLRVLAPALAARTRAGGRIALSGILASQATEVTDAYARWF
                     ATSVWQRDGEWVLVAGTRKAQA"
     gene            3028..4020
                     /locus_tag="VFO53_12305"
     CDS             3028..4020
                     /locus_tag="VFO53_12305"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HET8877103.1"
                     /translation="MSPEAKPQTPPSSATQTFTRCPGCATVFRVSAAQLALREGQVRC
                     GHCRAVFDAHDHRVALDAPRSDDDGLPDELAAGRPTVTLRSADALQPVPAVSHGPDAH
                     DAADTQSVDAVERDADAVPASNAAAAPAMAGATAHAAEPAATTEAADAPIVVEDTGLR
                     PARIEWKPRKPLRERPRLLHALAVVLLLAVLGAQALLEYRDALAAHAPFTRPILSALC
                     GPIGCSVGPLRDAGALSIDASDLRADPGHRGLLELSATIRNRAPYAIAWPYLELTLTD
                     ASDRVVARRAFEPADYAATDVRQGIAGNGEHVVRLFFDASETLQAGYRLYLFYP"
BASE COUNT          699 a         1407 c         1413 g          672 t
ORIGIN      
        1 atacgcaact cggcccgctc aaacgctcgg cgctcgacgg cctcctttcc cgtctgctgc
       61 cgcaggcgtg agcgcccgat gcccgagcgc gcggcgagcg tcggcggcta agtgccgcta
      121 acttctggac aaaagcgcct atctgcggca aactcgcgtc ttccttcgcg cgcgcagcgc
      181 atgaggccac gccatggatt tgcgcaagct caagacgttg atcgacctcg tcgaaacgtc
      241 ggggatcgcg gaacttgaaa tccaggaagg cgaagaacgc gtgcgaatca cccgcgcggt
      301 ggtgcagacg ccgcaaccgt tgatgttcca tccgcagcac cagctcgcga tgccggcgcc
      361 ggtcgcaatc gccccggtgc ccgaagcgcc agtcgcaccc gtcgacgaag ggcataccgt
      421 caagagcccc atggtcggca cgttctatcg ctgcgcgtcg ccgggtgctg caccgttcgt
      481 cgaagtcggc gacacggttg cccagggcga cacgctctgc atcgtcgaag cgatgaagct
      541 gatgaacgaa atcgaggctg acgcgagcgg aacgatcaag gccattctcg tcgagaacgg
      601 gcagccggtc gagttcgggc agccgctttt cgtgatcggc taggatggga cgacccccac
      661 cggctcggca atactgacat gccggctcgc agtcccgaca agctgttcga caaggtcctg
      721 atcgccaacc ggggggaaat cgcgctgcgc atccagcgcg cgtgccgcga gctcggcatc
      781 aagacggtgg tcgtgcattc cgaggccgac cgcgacgcca aatacgtggc gctcgccgac
      841 gagtcggtat gcatcggtcc ggcaccgtcg gcgcaaagct acctgaacat cccggcgatc
      901 attgcggccg ccgaagtcac cgacgcgcag gcaatccatc cgggctacgg cttcctgtcc
      961 gagaacgccg acttcgccga gaaggtcgag cgctcgggct tcgtgttcat cggcccgcgg
     1021 cccgacacga tccggctgat gggcgacaag gtgagtgcca aggtggcgat gaccaaggcg
     1081 ggcgtgccct gcgtccccgg atccgaaggc gcgctgccgg aggatccgaa ggaaatcgtg
     1141 cgcattgcgc gcggggtggg ctatccggtg atcatcaagg ccgcaggcgg aggcggcgga
     1201 cgcgggatgc gcgtcgtgca caccgaggcc gcgctgctgc ccgcggtcgc gctcacgcgc
     1261 acggaagcgc acgcggcatt cggcaacgcc accgtctact tggagcgctt tctcggcaat
     1321 ccgcgccaca tcgagatcca ggtcctcgcc gacgaattca agaatgccct gtgcctgttc
     1381 gagcgcgact gctcgatgca gcgccgccac cagaagatca tcgaggaggc gccggccccg
     1441 ggcgtcaatg cgcgtgcgct tgcaaggatt accgagcgtg ccgcggacgc atgccgcaag
     1501 ctcggctatc gcggcgccgg cacgttcgaa ttcctctatc aggacgagga attcttcttc
     1561 atcgagatga acacgcgcgt gcaggtcgag cacccggtga ccgagatgat caccggcatc
     1621 gacatcgtgc aggagcagtt gcggatcgcc gccggccaga agctgcgctt tcgccagcgc
     1681 gacatccagc ggcgcgggca cgccatcgaa tgccgcatca atgccgagga cccgtggacg
     1741 ttcgtgccgt cgcccggacg catcaccgcc tggcacgcgc ctggcggccc cggcatccgc
     1801 gtcgattcgc acgcctacca gaattatttc gtcccgccga actacgattc gatgatcggc
     1861 aaggtgatcg cctacggcga aacgcgcgag caggcgatcg caaggatgcg tgtcgcgttg
     1921 tcggagatgg tcgtcggcgg catccggacg aacattccgt tgcatcagga gctgctgttg
     1981 gacgagaaat tcctgcgtgg gggaacttcg atccattacc tcgaggagcg gctcgcgaag
     2041 cggaagcagg ccgcgtgagt tacctcgccg tacggttcga tgtcgaggcg gcacaggccg
     2101 atcgatggtc cgatgcgctg ctcgaagccg gcgcgctgtc ggtcgatgcc gccgatccgc
     2161 gtgcggggac gctcgatgaa agcgcgatct tcggcgagcc ggccgatgcg gcacccggct
     2221 ggtggccgat cacgcgcctg acggcgcttt gtgcgaacga gtgcgacccg gcgttgctcg
     2281 tgcgcaccgc ggcacgctgt gtcgagcagg cattgccggt ctttgaaacg cacgcgctgc
     2341 ccgaccagga ctgggtgcgc gcaacgcagg cgcagttcga gccgatccgc atcaacgaca
     2401 acctgtggat cgtgcccacg tggcgcgacc cgccgcggcc cggcgcagtc aacatccggc
     2461 tggatcccgg cctcgcgttc ggcaccggcg cgcatccgac cacgcgcctt tgcctcgcct
     2521 ggctcgccga gcaggttgcg cgcaccgatt cggtgctcga ttatggttgt ggctcgggcg
     2581 tcctcgccat cgctgcggca aggctcggcg ccgcgtgcgt cagcggcgtc gacatcgacg
     2641 cgcaagcggt tcgtgccagc gtcgacaatg cgctggccaa tggcgtgcat ggccagttca
     2701 ggcaggtcga cgcgctcgcg ccgcagacat tctcgatcgt ggttgcaaac atccttgcca
     2761 atccgctacg cgtgctcgcg cctgcgctcg ctgcgcgcac ccgcgccgga ggacgcattg
     2821 cgctgtccgg cattctcgcg tcgcaggcga cggaggtaac cgacgcgtat gcgcgctggt
     2881 tcgcgacgag cgtgtggcaa cgcgatggcg agtgggtgct cgtggcggga acgcgcaagg
     2941 cgcaggcgtg agcgcgcagg gtcgccccaa gcgcgatccc gagtccgcat cgcagcgaac
     3001 ccggtatcag catacaaggg tagtccagtg agccccgagg cgaagccgca gacgccgccg
     3061 tcttcggcaa cgcaaacgtt cacgcgttgc ccgggttgtg cgacggtttt tcgcgtcagt
     3121 gcggcgcaac tcgcgttgcg cgagggacag gtgcgctgcg ggcattgccg ggccgtattc
     3181 gacgcgcacg atcaccgcgt cgcgctcgat gcgccacgaa gcgacgacga tggtttgccc
     3241 gacgagctcg cggcgggccg gccgacggtg acgctgcgca gcgccgacgc gttgcagccg
     3301 gtgccagcgg tgtcgcacgg acccgatgcg cacgacgctg cggacacgca gagcgtcgac
     3361 gccgtcgagc gcgatgccga tgcggtgccg gccagcaacg cggcagctgc ccccgccatg
     3421 gccggcgcca cggcgcacgc tgcggagccg gccgccacga ccgaagccgc ggatgcgccg
     3481 atcgtcgtgg aagacacggg cctgcggccc gcgcgcatcg agtggaagcc gcgcaagccg
     3541 ctgcgcgaac ggccgaggct cctgcatgcg cttgccgtcg ttctgctgct cgccgtgctc
     3601 ggcgcgcagg cgctgctcga gtatcgcgat gcgctggccg cgcatgcacc gttcacgcgg
     3661 ccgattctct cggcgttatg cggcccgatc ggctgcagcg tgggtccgct gcgcgatgcg
     3721 ggagcgctgt cgatcgatgc atccgacctg cgcgccgatc ctggccatcg cgggctgctc
     3781 gagctcagcg caacgatccg caatcgcgcg ccgtacgcga tcgcatggcc ctacctcgag
     3841 ctcacgctga ccgacgcgtc cgatcgcgtc gtcgcgcgcc gcgccttcga gccggccgat
     3901 tacgccgcga cggacgtcag gcagggcatc gcaggcaacg gcgagcacgt cgtgcggctc
     3961 tttttcgatg cgagcgagac gttgcaggcg ggataccgcc tttatctgtt ctatccctga
     4021 gccgcgcttg caccgagctg gggcgcgcgt tacattgcta tgctccaagt tggtgcgtca
     4081 caccgcacca agatgcgcaa aagtgtgtat tattggttgt agattcaatg acttgcagtc
     4141 gccgaccggc tgggcatgga actcgctaat tccatccgcg tacgcgagcg c
//
LOCUS       DASSVX010000287        30666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_512570, whole genome shotgun sequence.
ACCESSION   DASSVX010000287 DASSVX010000000
VERSION     DASSVX010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 30666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30666
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512570"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VFO53_12310"
     CDS             complement(<1..1123)
                     /locus_tag="VFO53_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET8877104.1"
                     /translation="MHRPVIARLDAHVFRAPIATPVTNAFGTMTDRPMVLVSVEDGDG
                     CVGWGEIWSNFPVVGAEHRARLVTSVFAPLLVGKPLTDPADTFARLEASMASQVLQTG
                     EPGPFNACLAGIDQALTDLAARRAGLPLWRYLGGSRNSVRVYASGLGPEGAEAIAQAH
                     WQRGFRAFKLKVGFGRTRDLANLRALRDALPAAATIMVDANQAWSFDEARAMARALAD
                     ADLAWLEEPIAADQPLAVWQALANAAAPLALAAGENLCSTALLDAAVASGAVRVLQPD
                     IGKWGGYSRVLATARKAVGAGVRFCPHWLAGGVGLMASLHVCAAAGGGDGWVEWDANP
                     NPLRDGFPLPAVVDGRVVLTDAPGLGFVPDVAGLLSYEIA"
     gene            complement(1225..2280)
                     /locus_tag="VFO53_12315"
     CDS             complement(1225..2280)
                     /locus_tag="VFO53_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8877105.1"
                     /translation="MTSRGASVGLRNIVKRHGNVTALKGIDLDIRQGEFFALLGPSGS
                     GKTTTLRILAGLESHDAGSVIVDGRDVTGTDPGERDVAMVFQSYALYPHMSVYENIAF
                     PLRMVRTPSAEIDRAVRESASRVKIEHLLERRPGQLSGGQQQRCALARAIVRKPRLFL
                     LDEPLSNLDAKLRLETRVELRKLQRALDVTAVYVTHDQEEAMTVADRLAVFMDGMIVQ
                     VGTPEEVFRRPATIEVATFLGSPPMNLIPGRLSGNEVIIAGHRMRLDADHGRERDVLV
                     GVQPSRIELGAEGFPAQVDLTEQLGDSVIVDLQLGATRVRAKLPERTRLAEGAQVHVR
                     FGAADVHLFDPETHRRI"
     gene            complement(2277..3116)
                     /locus_tag="VFO53_12320"
     CDS             complement(2277..3116)
                     /locus_tag="VFO53_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET8877106.1"
                     /translation="MRRAFMRRLALACAAGVLLSVWGFPVIWALLTSFKTERDVLAYP
                     PKILFEPTLASYRDVLLGASSIVPNLVSSIVVASGATLLTLLFAVPAGYALARLELPG
                     KRASGFYVLATQMLPPVGLIIPYYLVLQRLGALDTYVGLIVIYLTFSLPFAIWLLVSY
                     FEDIPREMEEAALLDRAGRLRAFWYVILPQVRGGIAVTTVFIFLNAWNEFLFAVVLGG
                     NRVRPVTVAMFNFISVEQTRWASLAAGAMIAMAPVMLLGLIAQKHIVKGLTVGAVKGG
                     GRR"
     gene            complement(3113..3994)
                     /locus_tag="VFO53_12325"
     CDS             complement(3113..3994)
                     /locus_tag="VFO53_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8877107.1"
                     /translation="MSASHLQRQERRFALMLFTPALVLLLVTTSAPLVYLLWTSVQRI
                     DLTMPALSGFAGVDNYVKMGGDPRFWNSLWLTLIYTATTVVLQVVIGLSLALLVMQIP
                     RGQTVLRVAAILPIVLAPVVVGLFWRTLLLSPDFGLVDIVSRALGFGSHAWLGDPQLA
                     LVSVIAIHTWQWTPFAFLVILASLASLPPDVFEAARIDRANAWQRFRRITLPLLRPAI
                     VIVVIMRTMIALSAFAAIFAATGGGPGTATEILNLYAYRTSFTEFNLGYGAALAVVLL
                     AITLVISFLMFRMRRGR"
     gene            complement(4023..5384)
                     /locus_tag="VFO53_12330"
     CDS             complement(4023..5384)
                     /locus_tag="VFO53_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET8877108.1"
                     /translation="MHDPTSLFRFEEHDIYLPMLTLEELDAHKKGMTEVARNARQASR
                     FLDELVTTYAAPGAEGIVSGIPLAEKSSGSATGRLFLQSEPIASELPPSLASAKADNQ
                     IIAVVMHLTRHMPKRNVILVSKDINMRIKARALGLAAEDYFNDKVLEDTELLYPGTRE
                     LPADFWERHGKGMESWQQGGHTYYRLTGPLVPSLQLNEFLFLEKPDEAPFHAIVREIH
                     GKSGLLSTLKDYTHQKNNVWGIVARNREQNFALNLLMNPEIDFVTLLGQAGTGKTLLT
                     LAAGLMLTLEFKVYSEIIMTRVTVPVGEDIGFLPGTEEEKMNPWMGALEDNLDVLNKT
                     DDESGDWGRAATRDLIRSRIKVKSLNFMRGRTFINKFLIIDEAQNLTPKQMKTLITRA
                     GPGTKVVCLGNIAQIDTPYLTEGSSGLTYVVDRFKGWSHSGHVTLQRGERSRLADHAA
                     EVL"
     gene            complement(5668..6114)
                     /locus_tag="VFO53_12335"
     CDS             complement(5668..6114)
                     /locus_tag="VFO53_12335"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET8877109.1"
                     /translation="MAKVEDFEVASTDGTFRLSDHAGHPVVLYFYPKDNTPGCTTEGM
                     QFRDLYPKFAKLGAVVAGVSRDSLKSHASFADKMAFPFPLLSDADEALCTRFGVIKMK
                     NMYGKKVRGIERSTFVFDKAGNLAREWRGVKVPGHAAEVLAFVKTL"
     gene            complement(6101..7027)
                     /locus_tag="VFO53_12340"
     CDS             complement(6101..7027)
                     /locus_tag="VFO53_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET8877110.1"
                     /translation="MRLGLKIDVDTLRGTREGVPRLLDLLARHEVRATFLFSLGPDHT
                     GRALRRVFRKGFVDKVRRTSVVRHYGVRTLLYGTLLPGPDIGRRAEGILRRVRGEGHE
                     TGIHCWDHVRWQDGVATADAAWTRREMQRACERYADVFKEPPQTHGAAGWQMNAHAMR
                     LTQELGFAYCSDGRGRSPHLPVWRGELIRCPQFPTTLPTLDELIGAGNFTEENVAAHV
                     LERTREPPAAGHVFTLHAELEGARLANVFEQLLIGWKAQGFAPGPVRELCEGVEPLSL
                     PRCETAPAPIEGRAGTVLMQGDEFLGDVDGKG"
     gene            complement(7056..8099)
                     /locus_tag="VFO53_12345"
     CDS             complement(7056..8099)
                     /locus_tag="VFO53_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HET8877111.1"
                     /translation="MKRILILGVNGFIGHHLSKKIIASTDWEIYGMDMQTERIADLLD
                     ERRFHFFEGDITINREWIEYHVKKCDVLLPLVAIATPATYVREPLRVFELDFEANLPI
                     VRAAVRHRKRLVFPSTSEVYGMCSDAEFDPARSDLVLGPIDKPRWIYACAKQLMDRVI
                     HAYGQQDGLDYTLFRPFNWIGAGLDSINTAKEGSSRVITQFLGHIVRGEPIQLVDGGT
                     QKRAFTYIDDGVDALMKIIDNRAGIATGKIYNIGNPANNYSVRELAQMMLALALSYPE
                     YRESATATTIVETTAADYYGSGYQDVQNRVPKIDNTRADLDWEPSVGMDEALRRIFDA
                     YRGHVAEARALVE"
     gene            complement(8188..9135)
                     /locus_tag="VFO53_12350"
     CDS             complement(8188..9135)
                     /locus_tag="VFO53_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HET8877112.1"
                     /translation="MRGSTAREPDGRCAVVFAYHNVGDRCLRVLRAHGVDVPLVVTHT
                     DDPAETPWFARVADVAAELGLPCIAPADVNAPDVVDRIAALAPDFLFSFYCRRMLSPP
                     LLALARRGALNMHGSLLPFYRGRAPVNWAVLHGERRTGATLHYMAGKPDAGDIVAQQA
                     VPILPDDTAREVFDKVTVAAEICLDAALPGLIAGTAPRIRNDVARGSYFGGRTPEDGR
                     IDWSRPAIDIHNLVRAVAPPYPGARTSIGGVPSRVLKTRVLDAQAPAAAPALSARDRL
                     IARCGGGGTLAIDVLEVEGRIAAPREVRQRFGAVDVPLD"
     gene            complement(9183..10181)
                     /locus_tag="VFO53_12355"
     CDS             complement(9183..10181)
                     /locus_tag="VFO53_12355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8877113.1"
                     /translation="MTLPELSVVIPVYNEEDGLAALFGRLYPALDALRVPYEVIFVND
                     GSHDRSAALLREQFTARPDVTRVILFNANYGQHLAILAGFERVRGQRVVTLDADLQNP
                     PEEIGKLLAAMDAGADYVGGVRRAREDAWWRRAASRAMNRLRERITRIRMTDQGCMLR
                     GYSRDIVDAVAAAREVSTFIPALAYTFAHHPTEVDVLHEERAAGESKYSLYRLMRLNF
                     DLVTGFSLVPLQLFSVVGMIVAITSVILYAIVIVERAVIGGFLEGIKALWDRDILAFF
                     LIGCIMFGVGLLGEYVGRIYQQVRERPRYVIRAVLEREHEDVDLPHRPAESVRALR"
     gene            complement(10183..11307)
                     /locus_tag="VFO53_12360"
     CDS             complement(10183..11307)
                     /locus_tag="VFO53_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET8877114.1"
                     /translation="MADILKFASPLIDEATIAGVGDVLRSGHITSGVWVQRFETALSQ
                     FCDGRPVRVLTSATAAVEVALQLCGIGPGDEVITCAQSFFTVVNMIVKQGATPVFVDC
                     DLVTRNIDLAQMESRIGPRTRAIIPTHWPGSLVDMDALYALARRHGVRVIEDAALVIG
                     SHFNGRAIGAFGDLVTFSFHPNKNITSIEGGALVCNDDAEARRVEVLRFHGIERLPDG
                     TRDVAFPGGKFNLPDVNARIGVEQLARLPQFLAHRRALVLRYFERFPADRGCVLPPRP
                     ADGDGQSWNMFCILLPDAMKDSRKDFRDALAARAIATGVSYEALHLSTIGRRFGYRRG
                     DLPNTERIALRTVSLPLHAAMDAADVDRVCAACADLLPRH"
     gene            complement(11307..11678)
                     /locus_tag="VFO53_12365"
     CDS             complement(11307..11678)
                     /locus_tag="VFO53_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET8877115.1"
                     /translation="MSTAAFLFLFTGVLLNAAAQLLLKGGTNRLGVITLNAGNWAATL
                     TRMASEGYFIAGAALYGVSIIVWIIGLSRVPVSIAYPMLSLGYIVNAIAAHYLFGEAV
                     TAQRWLGIGFIVAGVYLVARS"
     gene            complement(11762..13465)
                     /locus_tag="VFO53_12370"
     CDS             complement(11762..13465)
                     /locus_tag="VFO53_12370"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HET8877116.1"
                     /translation="MPPESPTLAALSRRSWIALALVFALAWFANLDVRKLQHPDEGRY
                     AEIAREMVASGDWVTPRLDGLKYFEKPPLQYWLTAASFKAFELDEWTARLPGAVAGFA
                     TVAVVAGTGAVIASPAAGLYAGLALAGSVWLFGISHLVTLDALLAVWLTLSLCAFLRA
                     QHAAASPILARRWMLLAYAAAAGGVLTKGLVALLIPFATLIVYSLATRDRGPWKRLHL
                     RAGLAVLLVLAAPWFVLVSLRNPEFARFFFIHEHFERFLTTEHRRIGAWWYFVPMFVV
                     GFLPWTGLFLWRLRRGWRDAVVDARGFAWVRFCLVWAAFVFVFFSISGSKLPSYILPM
                     FPAAALVLGRQLARTRVATLAVFTAVLGAVTLALWLVALAGWPRIADALADPRTPRAL
                     YDALGHWVKLALGIAAGGYIAGYLAFRRNDERGRTLGIVALSLATILAMQATFSGSDV
                     FRATRSAADLVTTLENAANPPYDPDAPFFQVRMYDQTLPFYLGRTTTLVEYRDELGPG
                     LDAEPGRGIAHVSDWIARWRSLPQGYALMAPQTHATLAQEDVPMRVVASDSRRVLVAR
                     R"
     gene            complement(13574..13855)
                     /locus_tag="VFO53_12375"
     CDS             complement(13574..13855)
                     /locus_tag="VFO53_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HET8877117.1"
                     /translation="MIAHPVARARSYVLVWLALIGLLVVTLASAYVPLGWANSAINLA
                     IAVAKALLVAFFFMHLRSAHYMLRIAAMAGFFWLAILIGLSLTDFAIRY"
     gene            complement(13852..14493)
                     /locus_tag="VFO53_12380"
     CDS             complement(13852..14493)
                     /locus_tag="VFO53_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HET8877118.1"
                     /translation="MPSVVIVARQFDDAAQQRTASDLGMWVFLATEILFFGALFAAYT
                     ATRLHDPQAFAIASRLTNVTLGSINTAVLLTSSLMMALAVRATKLGLRRAALGFLCAT
                     AVLGIAFLAIKFTEYGLDYAGHLVPVLDFAHQGPHAGGVENFFYLYFFMTGLHSVHMI
                     IGIAVVAVLAIGTARRRFTADYFTPVELGGLYWHLVDIVWIFLYPLLYLVSRT"
     gene            complement(14542..16152)
                     /gene="ctaD"
                     /locus_tag="VFO53_12385"
     CDS             complement(14542..16152)
                     /gene="ctaD"
                     /locus_tag="VFO53_12385"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET8877119.1"
                     /translation="MSTVTLPLPRSYLEEGFSLWSWIASVDHKRIAILYAISITLFFF
                     VGGAAATLIRMELITPAGDLVSAETYNKLFTMHGVIMVWFFLIPSIPSTFGNFLVPLM
                     IGARDLAFPRLNLASWYLYIAGGLFTVGALVAGGVDTGWTFYTPFSTMFSNTHVIAAV
                     CGVFIVGFSTILTGINFIVTIHKMRAPGMTWFRLPLFIWAIYATSIIFVLATPVLAMT
                     LFLVAIERFAGVGVFDPALGGDPLLFQHLFWFYSHPAVYVMILPAMGVASEIITCYAR
                     KDIFAYRSMAFAVLAIAIIGFLVWGHHMFVSGQSVYAGMVFSILTFLVAIPSAIKVFN
                     WTATLYKGSIRFDAPMLYLLGFVGLFTIGGMTGLFLASLALDVHLHDTYFVIAHFHYI
                     MVGGSVMAFLGAVHFWWPKITGRMYSDRWARFAAITVFFGFNLTFFPQFLLGYLGMPR
                     RYHEYPPEFQVLNVLSSAGASILAVGYLLPLFYLLWSLKHGARAGPNPWRATGLEWQT
                     ASPPPVANFAAIPIVTAPPYQYSAMEDR"
     gene            complement(16184..17146)
                     /gene="coxB"
                     /locus_tag="VFO53_12390"
     CDS             complement(16184..17146)
                     /gene="coxB"
                     /locus_tag="VFO53_12390"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET8877120.1"
                     /translation="MWDFPLHPPADSLLATRVDHLFYAWLALAGVVTVAVFFLIVYFS
                     VKYRHGSRADRDMPSRRTLRRESHRIEIAWIGAPLILFLGMGFWAAGLYYAHAAPPAD
                     ATEVYAVGKQWMWKLEHTGGQREIDELHVPAGRPIKLVMTSQDVIHSFFMPVFRIKQD
                     VLPGRYTILWFTADKPGDYHVFCTQYCGTDHARMIGHVVVMQPAAFERWLTSGNASQS
                     MAAEGAARFRQYGCSGCHGASASVHAPKLEGLYGRRVQLSDGSSVVADERYIHDSVML
                     PRKDVVAGYEPIMPSFQGEIAEDDLLEIVEYIKSLRGAEPGTVR"
     gene            complement(17153..17932)
                     /locus_tag="VFO53_12395"
     CDS             complement(17153..17932)
                     /locus_tag="VFO53_12395"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET8877121.1"
                     /translation="MIARACCAFLCTLAFATGALAHLTPADASQRAGFEQRLSAQLPA
                     ALAFRDEADRNVRLGDYFGATPIVLVFAWYGCTTLCPTVVGNVAAALGRSGLAHGSYR
                     VLVASIDARDAPADARRMKRRYLAQANVDAGAWHLLTGSAADIAALTHAAGFRYAYDD
                     ATHQYAHPAGIVLLTPQGSIAHYFFGFDFTPAALHDAVDAAAADEIGSPVERLLLVCF
                     HFAPAGPYSALVMQALRIGSVALLVAGLALFVAMRRRTALR"
     gene            complement(17929..18321)
                     /locus_tag="VFO53_12400"
     CDS             complement(17929..18321)
                     /locus_tag="VFO53_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877122.1"
                     /translation="MAEAQGRVRVNAPPLRGAAHETADVDGRRVWQTAAALAATIVAA
                     GFIASGVVAFFHHETGRPLSPTDAPPTAMPSPRLQSAPALDLATLRAQKRAMLNEYRW
                     IDRDKGIVRIPIERAMALLIARGAARQK"
     gene            complement(18314..19513)
                     /locus_tag="VFO53_12405"
     CDS             complement(18314..19513)
                     /locus_tag="VFO53_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877123.1"
                     /translation="MSLRAEPVRHAEGVAWIVGAAGAIGFAAAAYADRATAFQSYLFV
                     WWFLLGIPLGSIAMLAVHDLTGGGWGEVIRGPLDAAARLLPLSLLLVVPLLFGLRDMY
                     AWARPDAVAADPLLQDKRWYLSVDFFLLRNAIYFVIWLALGHLLRKWAFARHAGATLR
                     DKERLRAIAAIGLLLYAGTVTFAGVDWIMSLSPRWFSTTFGFLVGIGQTMCAFSFAIV
                     CAAWRLRDAVGSQANLASTGPESSRLPGDVPGLFQDLGNLLLMFVLTWAYLAFTQYLI
                     TWAEDLPNEIAWYLPRVTTSWHDVALLLVAIHFALPFLVLLSRRAKRVPRALGALAAL
                     LLFAHVVDSYWLVVPAHRPSGISVTWSDVAAIAALGGLWLAVFVRTARGAPDVTAPVS
                     SEATHHG"
     gene            complement(19510..20211)
                     /locus_tag="VFO53_12410"
     CDS             complement(19510..20211)
                     /locus_tag="VFO53_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET8877124.1"
                     /translation="MRRPATRLVAVTLTRTALAPVALALALSGCEKAMQDMYNQPKYK
                     PLGASALWADGRASRPDVPNTLAYSGGATAGTSSGRLGTLPLAASEAPTYPVDDRGRV
                     KAALTSGSPQPLLAANPLPMDRPTLERGRERFDIYCSPCHSVAGDGDGMIVRRGFPSP
                     PSYHTDRLRNAPDAHFYSVITNGYGMMYSYADRVAPHDRWAIVAYIRALQLAQDARIG
                     DVPPERRAELAKGSK"
     gene            complement(20214..20846)
                     /locus_tag="VFO53_12415"
     CDS             complement(20214..20846)
                     /locus_tag="VFO53_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HET8877125.1"
                     /translation="MSAAEPPRGANCAPSGGSAAAKPQAWGDHASADRELYGLLAEFR
                     DGDALVDATARATAAGYRNVDAYAPFSVPGLADALGFRRDRVALITLLGGIAGGVGIY
                     FLQWYSAVIDYPINAGGRPLHSWPAFIPATFECTVLGAALAAFFGFLILNGLPKLHHP
                     IFNADDFDLASRNRFFLCVEAADPRFERAATQRFLESLDPLRVTEVASDS"
     gene            complement(20843..22195)
                     /gene="nrfD"
                     /locus_tag="VFO53_12420"
     CDS             complement(20843..22195)
                     /gene="nrfD"
                     /locus_tag="VFO53_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET8877126.1"
                     /translation="MDESRLEVLHGRYSYASISDKISDIVLTRPTHWGFYAGLALCTA
                     LTVLFVVAIVYLFTTGVGIWGVDIPVAWAFAITNFVWWVGIGHAGTFISAFLLLLRQK
                     WRTSVNRFAEAMTIFAVAMAGVMPILHLGRPWFFYWLVPYPDIMNVWPQWRSPLVWDI
                     FAIGTYIVVSILFWYMGLIPDLATLRDRTDHRVKQIVYGLFALGWRGEARQWRRFDTA
                     YLLMAGLATPLVISVHSVVSLDFSIGNTPGWHSTIFPPYFVAGALFSGFAMALTISIP
                     LRAVFGLKDFITARHLDNIAKVMLVCGLLVAYSYVVEIFMAFYSGDRFEKYMIVNRMT
                     GPYAPIYWTVVFCNVLVPQAMWWKRVRLSTLTLFAISIVVNIGMWLERFMIIVTSLHR
                     DFLPSSWDIYYPTFWDWATLAGSLGAFGWLFLMFVRFLPAISISEMRKLVLEKREGET
                     "
     gene            complement(22230..25130)
                     /locus_tag="VFO53_12425"
     CDS             complement(22230..25130)
                     /locus_tag="VFO53_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-cluster-containing hydrogenase"
                     /protein_id="HET8877127.1"
                     /translation="MWRSLDELTDGDAIAERLAHEHPSWGALYRVDRREFLKLMSASL
                     ALAGLAGCTREPQERILPYVNAPLGQVAGEPRFYATANAVGGFAMGVLVESTMGRPTK
                     VEGNPLHPASLGSTDVFAQASVLDLWDPDRSQTVTHRGVVSTWDGFAAALARRLRDLG
                     ARQGDGLRILTETVTSPTLAAQLQALLARYPRAQWHQYEPVNRDNAYDGARLAFGTPL
                     DVRHRFDRAAVVLSLDADFLGTGPDRVRAARDFVSHHATHGAIGESNRLYAIAATPSL
                     TSAYADHALALRGGRIEAAARWIAKRLGVPGAAGDSVAGAPDAWLAACAHDLDTHRGA
                     SLVVAGERQPPQVHALAHAMNAYLGNAGVTVSHAEPAVANPVNQLQSLAALVADIGAH
                     RVDTLVIVGGNPVYDAPRELRFAENLGNVAMGVRLGLHEDETSAHCEWHLPAAHFLEA
                     WSDARAYDGTVTIIQPLIAPLYDGRSAHEVVSMLADGTPRVGYAIVREFWQPKLGPDP
                     DIAWQTALRNGVVAGSALPERSVALRSEALAGTTAQAAPNAGEGSLDLQFVPDASVHD
                     GRYANNPWLQELPRPLTKITWDNAVLVAPALAARLGLANDDVVELRRAGASVSGPVWV
                     LPGHPEDAVTVSLGYGRSRAGRVGDAHGFDAYALRSADAAWFAAGLSLRKTGRRYKLA
                     TTQHHHSMEGRDIVRVATLADFRRDPRAAGAPQRPNGTLYEPFAYDGYKWGMTIDLGA
                     CIGCNACTIACQAENNIPTVGKAQVELGREMHWIRVDRYYDGAPNAPRTHFQPVPCMQ
                     CQRAPCELVCPVEASVHDSEGINVQVYNRCVGTRFCSNNCPYKVRRFNFLQYSDEHTE
                     SLKGQRNPEVTVRMRGVMEKCNYCLQRIANGRIEADKQDRRIRDGEVVTACQAACPTQ
                     AITFGDLNDPASAVNARKSSPLDYALLAELNTEPRTSYLAKLRNPNPDLGES"
     gene            complement(25156..25806)
                     /locus_tag="VFO53_12430"
     CDS             complement(25156..25806)
                     /locus_tag="VFO53_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET8877128.1"
                     /translation="MAQVFQPRLVLALKLGLLAVLGMVFAALLAWRILIAPHPAIGTP
                     VEQVVAFSHKHHVGDDGIDCRYCHTSVEQSAFAGIPPTHTCMTCHSQLFTQAPILAPV
                     VQSLASGRPLVWNRVHDLPGFVYFNHGIHIAKGVGCATCHGPIDRMPLTWRVAPLQMQ
                     WCLDCHRTPEKYLRPVEHVFDMDWKPAEDQMALGTRLVAAYHIHKSTLTDCSTCHR"
     gene            complement(25813..25962)
                     /locus_tag="VFO53_12435"
     CDS             complement(25813..25962)
                     /locus_tag="VFO53_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877129.1"
                     /translation="MHDRAIDYIAERVAYAAIRRALPFLETIIGTSNAARSIGASRSS
                     RHRPR"
     gene            26121..26660
                     /locus_tag="VFO53_12440"
     CDS             26121..26660
                     /locus_tag="VFO53_12440"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8877130.1"
                     /translation="MRAHAAPLTELPALSVAIAAARAADLPLVARLAHEIWHRHYPGI
                     LPVAQIDYMLARGYSREALTDYLTRDGAGLALAMHAETAVGFVGWYPLEPPGTMKLDK
                     LYVLPEHHGTGIGRALIDHVITEARAAACRAVTLNVNRGNAGSIRAYERCGFAITERG
                     DFPIGNGYVMEDFIMVRQL"
     gene            26668..28053
                     /gene="cysS"
                     /locus_tag="VFO53_12445"
     CDS             26668..28053
                     /gene="cysS"
                     /locus_tag="VFO53_12445"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET8877131.1"
                     /translation="MASLALYDNYARSLREFSPLDPGRPVGLYTCGPTVYDYQHIGNY
                     RTFLFEDLLKRVLRWNGYRVRHVMNITDVGHLTSDADTGEDKMEKGARRTGRSAWEIA
                     ALYTQAFVDDLRALNILPADVLSRATDHIAEQIAFIADLEQRGYTYRTSDGIYFDTSR
                     QADYGYLARLDRAGLQAGRRVDVGEKRHATDFALWKFSPADSTRQMEWDSPWGKGFPG
                     WHIECSAMAQRYLGDWFDIHCGGEDHIPVHHTNEIAQTEARAGTRLANFWMHGYFLLA
                     NDAKMAKSAGEFLRIAALVERGYDPLAFRYLCLTGHYRTQLNFTWEALDAAATGLARM
                     RSAFHALPFDAAATIPQDLLDRFTALVNDDLNAPRALALAWETLRGDLRLAVKRAALA
                     RFDDVFGLGLAQWQPAEDPIPAAIAALGEARADARKARNWAEADRLRAALQEAGFDVE
                     DTPDGYSLKRK"
     gene            complement(28066..28782)
                     /locus_tag="VFO53_12450"
     CDS             complement(28066..28782)
                     /locus_tag="VFO53_12450"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET8877132.1"
                     /translation="MNLSRSDRRERLDRLAAEFALGTSPPRVRRRLIAISRRDRVVAA
                     ALSEWERRVAVLADQVPPVTPPPRAWRNIVARLGLDPEGTSKRETAWWQRLAFWRTLA
                     IASLAGVIAIGIGEWRRAPVIVPAPLVVVLSDADAKVGLIATATRGERYLTLKTVSNA
                     KPGPDKAYELWALPQSGAPHALGVIPAGDVVRVPLDNPVDETLSNVSTLAVSLEPPGG
                     SPTGKPTGPVLYSGRIERMY"
     gene            complement(28779..29387)
                     /locus_tag="VFO53_12455"
     CDS             complement(28779..29387)
                     /locus_tag="VFO53_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET8877133.1"
                     /translation="MTGVDPQPGPNARSEKLAALLARIALGDQRAFAELYGLASAHLY
                     AVAVRIMRDGPIAEEMLQEAFVSVWHHAGTYDAAKSQPLTWLTSIVRNRCLDWLRRRE
                     VDTETLTPRDDDDSPAWEPPSDGPTPVDLLLAAADARALRGCIDGLEPGPRQAIALAF
                     FRGLSHRELAAHLREPLGTVKSWVRRGLATLRRCLDAAGAAE"
     gene            complement(29384..29947)
                     /locus_tag="VFO53_12460"
     CDS             complement(29384..29947)
                     /locus_tag="VFO53_12460"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET8877134.1"
                     /translation="MGHRLSRIVTRTGDSGTTGLGDGSRVAKDSPRIEAIGAVDELNS
                     TIGVALAESLPADVHALLTDIQHDLFDLGGELSIPGHAAVTPAHIARIEAAVEHYNAN
                     LPMLKEFILPGGTRAAALAHVARTVCRRAERTLIALAAGDSVSTSSRIYLNRLSDLLF
                     VLARVLNRAAGAPDVLWQKDRMRGANA"
     gene            30106..>30666
                     /locus_tag="VFO53_12465"
     CDS             30106..>30666
                     /locus_tag="VFO53_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET8877135.1"
                     /translation="MSTQRMYETHLDRRPANFAPLTPLSFIARTAAIWPHRTAVVHGP
                     RRYTWAGTYARARRLASALAHRGVGVGDTVAAMLSNTPEMIECHFGVPMTGGVLNTLN
                     TRLDAEALAFMLEHGEAKVLITDTEFAPAIGAALARLRTKPLVIDVVDALGPGGDLIG
                     ESDYETFIAHGDADFAWQPPADEWDAI"
BASE COUNT         5341 a        10142 c        10602 g         4581 t
ORIGIN      
        1 acgcgatctc gtacgaaagg agcccggcga catcgggcac gaagccgagc ccgggcgcgt
       61 cggtgagcac gacgcggcca tcgacgaccg cgggcagcgg aaagccatcg cgcagcggat
      121 tggggttcgc gtcccattcg acccatccgt cgccgccacc ggccgctgcg cacacatgca
      181 gcgacgccat caagcccacg ccgcccgcga gccagtgcgg gcagaagcgc acgccggcgc
      241 cgaccgcctt gcgtgccgtc gcgagcacgc gcgaataccc gccccatttg ccgatatcgg
      301 gttgcagcac gcgcaccgca cccgatgcaa cggccgcgtc gagcagcgcc gtgctgcaca
      361 ggttttcccc ggccgccaac gcgagcgggg ccgcagcgtt cgcgagcgcc tgccagacgg
      421 cgagcggttg atcggcggcg atgggttcct cgagccatgc gaggtcggcg tcggcgagcg
      481 cgcgcgccat cgcccgtgcc tcgtcgaacg accaggcctg gttcgcgtcg accatgatcg
      541 tagctgccgc gggcagcgca tcgcgcaacg cgcgcaggtt cgcgagatcg cgcgtacgcc
      601 cgaagccgac cttcagcttg aacgcacgga atccgcgctg ccaatgcgcc tgcgcgatcg
      661 cttcggcgcc ttctgggccg aggccgcttg cgtaaacgcg cacgctattg cgcgagccgc
      721 caaggtagcg ccacagcgga agtcccgcgc ggcgcgcggc gagatcggtc agcgcctggt
      781 cgatgccggc aaggcaggcg ttgaacggcc cgggctcgcc ggtctgcagc acctggctcg
      841 ccatggacgc ttcgagccgg gcaaacgtat cggccggatc ggtcagcggt ttgccgacca
      901 gcagcggcgc aaaaacgctc gtcacgaggc gcgcgcggtg ctcggcaccg acgaccggga
      961 aattgctcca gatctcgccc cagccgacgc agccgtcgcc gtcttcgacg gacaccagca
     1021 ccatcggccg atcggtcatc gtgccgaacg cattggtcac cggcgtcgcg atcggcgcgc
     1081 gaaagacgtg cgcgtcgaga cgagcgatga cggggcggtg cacggcgctt gtatgatccc
     1141 cccacgctcg cggcttcgcc gcttcgctgc cccccgaggg ggcgcaattc gcactttggg
     1201 gcggcccggc agcgctcatg cgagtcagat gcgccgatgc gtttcgggat cgaacaggtg
     1261 cacgtccgcc gcgccgaagc gcacgtgcac ctgcgcgcct tcggcaaggc gggttcgctc
     1321 gggcagcttc gcccgcacgc gtgtcgcgcc gagctgcagg tcgactatca cgctgtcgcc
     1381 gagctgctcg gtcaaatcga cctgcgccgg aaagccttcg gcgccgagct cgatgcgtga
     1441 cggctgcacg ccaaccagca cgtcacgttc gcggccgtgg tcggcatcga ggcgcatccg
     1501 atgcccggcg atgatcactt cgttgccgct caagcgtccg ggaatgagat tcatcggcgg
     1561 gctgccgaga aacgttgcga cctcgatcgt cgccggccgg cggaacactt cctccggcgt
     1621 gcctacctgc acgatcatgc catccatgaa taccgcgagc cggtcggcga ccgtcatcgc
     1681 ctcttcctgg tcgtgcgtca cgtacaccgc agtgacgtcg agcgcgcgct gcagcttccg
     1741 cagctcgacg cgggtttcga ggcgcagctt cgcgtcgaga ttcgacagcg gctcgtcgag
     1801 caggaacagc cgcggcttgc gcacgatcgc ccgcgccagc gcgcagcgct gctgctggcc
     1861 gcccgacagc tgcccgggac gccgctcgag gagatgctcg atcttgaccc ggcttgccga
     1921 ctcgcgcacg gcgcggtcga tctcggcgga tggcgtcctc accatgcgca gcggaaacgc
     1981 gatgttctcg tacacgctca tgtgcgggta cagcgcgtag ctctggaaca ccattgcgac
     2041 gtcgcgctcg ccgggatcgg ttcccgtcac gtcgcgcccg tcgacgatga cgctgcccgc
     2101 gtcgtgcgat tcgagcccgg cgagaatgcg cagcgtcgtc gtcttgcccg agcccgacgg
     2161 accgaggagt gcaaagaatt cgccctggcg gatgtcgagg tcgatgccct tgagcgccgt
     2221 gacattgccg tggcgcttca cgatgttgcg caagccaacg ctggcgccgc ggctcgtcat
     2281 cgccggcctc cgcccttgac ggcgccgacg gtcagcccct tcacgatgtg cttctgcgcg
     2341 atgaggccga gcagcatgac cggtgccatt gcgatcatcg cgccggcagc cagactcgcc
     2401 cagcgcgtct gctccacgga aatgaagttg aacatcgcga cggtgaccgg acgaacgcga
     2461 ttgccgccga gcacgacggc gaacaggaac tcgttccatg cgttgaggaa gatgaacacg
     2521 gtggtcaccg cgatgccgcc gcgcacctgc ggcaggatca cgtaccagaa cgcgcgcaac
     2581 cgcccggcgc ggtcgaggag cgccgcctct tccatctcgc gcggaatgtc ctcgaagtac
     2641 gacacgagca gccagatcgc gaacggcaac gagaacgtga ggtagatcac gatcaggccg
     2701 acgtatgtgt cgagtgcgcc gaggcgctgc agcacgaggt aatacgggat gatcaatccg
     2761 accggcggca gcatctgcgt tgccagcacg tagaagcccg aggcgcgctt gcccggcagc
     2821 tcgagccgcg ccagggcata accggccggc accgcgaaga gcagcgtcaa aagcgtcgcg
     2881 ccggacgcga cgacgatgct cgacacgagg ttcggcacga tgctcgacgc gccgagcagc
     2941 acgtcgcgat agcttgcaag cgtcggctcg aagaggatct tcggcggata ggcgagcacg
     3001 tcgcgctcgg tcttgaacga cgtgagcagc gcccagatta ccgggaatcc ccagacggac
     3061 aacagcacgc cggccgcgca ggcgagtgcc agccggcgca tgaatgcgcg cctcatcgcc
     3121 cgcggcgcat ccggaacatg aggaacgaga tgacgagggt gatcgccaga agcacgacgg
     3181 cgagcgccgc gccgtaaccg aggttgaact cggtgaacga cgtccggtag gcgtagagat
     3241 tgaggatttc ggtcgcggtt ccgggacccc cacccgtggc ggcgaatatc gcggcgaacg
     3301 ccgacagcgc gatcatcgtg cgcatgatga ccacgatcac gatggccggc cgcagcagcg
     3361 gcagcgtgat gcggcgaaag cgttgccacg cgttcgcgcg gtcgatgcgg gcggcctcga
     3421 acacgtcggg cggcagcgac gcgaggctcg cgaggatcac gaggaaggcg aacggcgtcc
     3481 attgccacgt gtggattgca atgaccgaca cgagcgcaag ctgcgggtcg ccgagccatg
     3541 cgtggctgcc gaagccgagg gcgcgcgaca cgatgtcgac gagcccgaaa tcgggcgaca
     3601 gcagcagcgt gcgccagaac aggccgacga cgaccggcgc gagcacgatg ggaagtatgg
     3661 ccgcgacgcg caataccgtc tggccgcggg ggatctgcat cacgagcagc gcgagagaca
     3721 ggccgatcac cacctgcagc acgacggtgg tggccgtata gatcagcgtg agccacagcg
     3781 aattccagaa gcgcgggtcg ccgcccattt tcacgtagtt gtcgacgccc gcgaagccgg
     3841 acagtgccgg catcgtgagg tcgatgcgct gcacgctcgt ccacagcaga tagacgagcg
     3901 gcgcgctcgt cgtgacgagc agaagcacca gcgccggcgt gaacagcatc agcgcgaagc
     3961 ggcgctcctg ccgctgcaga tgcgacgcac tcacgagctt gtctgcggcg gcccttcggc
     4021 tctcacagca cttcggctgc gtgatcggcg aggcgcgacc gttcgccgcg ctgcagcgtc
     4081 acgtgtcccg aatgcgacca gcccttgaag cggtcgacga cgtaggtgag gcccgagctg
     4141 ccttcggtca ggtacggcgt gtcgatctgc gcgatgttcc cgagacagac gaccttcgtg
     4201 ccgggccccg cacgcgtgat cagcgtcttc atctgcttgg gcgtcaggtt ctgcgcctcg
     4261 tcgatgatca ggaacttgtt gatgaacgtg cgaccgcgca tgaaattgag gctcttcacc
     4321 ttgatgcgcg agcggatgag atcgcgcgtt gcggcgcggc cccagtcgcc gctttcgtcg
     4381 tcggtcttgt tcagcacgtc gaggttgtcc tcgagcgcgc ccatccacgg gttcattttc
     4441 tcttcctcgg tccccggcag gaagccgata tcctcgccca cggggacggt gacgcgcgtc
     4501 atgatgatct ccgagtacac cttgaattcg agcgtcagca tgagccctgc ggcgagcgtc
     4561 agcagcgtct tgccggtgcc cgcctgcccg agcagcgtga cgaagtcgat ctcgggattc
     4621 atcagcaggt tgagcgcgaa gttctgctcg cgattgcgcg caacgatgcc ccacacgttg
     4681 ttcttctggt gcgtgtagtc cttgagcgtc gacagcagcc cgctcttgcc atggatctcc
     4741 ctgacgatcg cgtggaacgg cgcttcgtcg ggcttctcga gaaacaggaa ttcgttgagc
     4801 tgcaacgacg gtacgagcgg accggtgagc ctgtagtacg tgtgcccgcc ctgctgccac
     4861 gactccattc ccttgccgtg acgctcccag aaatcggcgg gcagctcgcg cgtgccggga
     4921 tagaggagct cggtatcctc gagcaccttg tcgttgaagt aatcctccgc ggcgaggccg
     4981 agcgcgcgcg ccttgatccg catgttgatg tccttcgaca ccaggatcac gttgcgcttc
     5041 ggcatgtgcc gcgtcaggtg catcacgacg gcgatgatct ggttgtcggc cttggccgaa
     5101 gcgagtgacg gcggaagctc gctcgcgatg ggttcggatt gcaggaacag ccggccggtg
     5161 gcggaaccgc tcgatttctc ggcgagcggg atgccgctca cgatgccttc ggcgcccggt
     5221 gccgcgtacg tggtgacgag ctcgtcgagg aagcgcgatg cctggcgcgc attgcgcgcg
     5281 acttcggtca tccctttctt gtgcgcgtcg agctcctcga gcgtgagcat cggcaggtag
     5341 atgtcgtgtt cctcgaagcg aaacaggctc gtcggatcgt gcatcagcac gttcgtatcg
     5401 agcacgaaca gcttggcgga ggcggaggat ccggcgcggg ccggcttgcg ggatggcagg
     5461 agatgaagcg tggagggagc ggggcgcagc ttgcgttcga ccatgggcct cctgtggggc
     5521 gattcgtgcg gacggacgat gcgccgtctg cgctgtgggt taatgtaggc cggatcgaca
     5581 ccgcatacaa gtgctagcgg tcgggtcgtg ttcgggcatc ggattttgtg gtgcaaagcg
     5641 caaaacgagg gccgagcctt cgtgcgctca gagcgttttc acgaacgcga gcacttccgc
     5701 ggcatgtccc ggcaccttca cgccgcgcca ctcgcgcgcg agattgcccg ccttgtcgaa
     5761 cacgaacgtg ctgcgctcga ttccgcgtac cttcttgccg tacatgttct tcatcttgat
     5821 cacgccaaaa cgcgtgcaga gcgcctcgtc ggcatcggac agcagaggaa acgggaacgc
     5881 catcttgtcg gcaaagctcg cgtgcgactt cagcgaatcg cgcgacacac cggccacgac
     5941 ggcgccgagc ttggcgaact tcggatagag gtcgcgaaac tgcatgccct cggtggtgca
     6001 gcccggcgtg ttgtccttcg gatagaaata cagcacgacc gggtgccctg cgtgatcgga
     6061 aagacggaac gttccatccg tggacgcaac ctcgaaatcc tcaacctttg ccatcgacgt
     6121 ctcccagaaa ttcatcgcct tgcatcaaaa cggttcccgc gcgtccctcg atcggtgccg
     6181 gcgccgtctc gcagcgcggc agcgaaagcg gctcgacgcc ctcgcacagc tcgcgcacgg
     6241 gaccgggcgc aaacccctgc gccttccagc cgatcagcaa ctgctcgaat acgttcgcaa
     6301 gccgcgcgcc ttcgagctcc gcgtgcagcg tgaaaacgtg ccccgccgcc ggtggctcgc
     6361 gcgtgcgctc gagaacgtgc gcggcgacgt tctcctcggt gaagttaccg gcgccgatca
     6421 attcgtcgag tgtcggcagc gtcgtcggaa actggggaca gcggatgagc tcaccgcgcc
     6481 ataccggcag atgcggcgag cgtccgcggc catccgagca gtaagcgaag ccgagttcct
     6541 gcgtgaggcg catcgcatgc gcgttcatct gccatcccgc ggcgccatgg gtctgcggcg
     6601 gctccttgaa tacgtcggcg tagcgctcgc acgcgcgctg catctcgcgg cgcgtccacg
     6661 cggcgtcggc ggttgcgacg ccatcctgcc agcgcacatg atcccagcaa tggatccccg
     6721 tttcgtgtcc ctcgccgcga acgcggcgca ggatgccttc ggcgcgccgt ccgatgtccg
     6781 gcccgggcag cagcgttccg tacaggagcg tgcgcacgcc gtagtgccgc acgaccgacg
     6841 tgcggcgcac cttgtcgacg aatcccttgc ggaagacgcg gcgcagcgca cggccggtgt
     6901 gatcgggacc gagcgaaaac aggaatgtcg cgcgcacctc gtgccgggca aggaggtcga
     6961 gcaggcgtgg aacgccttcg cgcgtgccgc gcagcgtgtc gacgtcgatc ttgaggccga
     7021 ggcgcacgaa atcaggcagg cgaggatcgc agcactcact ccaccaacgc ccgcgcctcc
     7081 gcgacgtgcc cgcgatacgc gtcgaagatg cggcgcaacg cctcgtccat gcctacgctg
     7141 ggttcccagt cgaggtcggc acgcgtgttg tcgatcttcg gcacgcggtt ctggacgtcc
     7201 tggtagccgc tgccgtagta gtcggccgcg gtcgtttcca cgatcgtcgt cgccgtcgcg
     7261 ctttcacggt actcgggata ggagagcgcc agcgcaagca tcatctgcgc gagctcgcgc
     7321 accgagtagt tgtttgccgg gttgccgatg ttgtagatct tcccggtcgc gatgccggcc
     7381 cggttgtcga tgatcttcat cagtgcgtcg acgccgtcgt ctatgtaggt gaacgcgcgc
     7441 ttctgcgttc cgccgtcgac gagctggatc ggctcgccgc gcacgatgtg cccgaggaac
     7501 tgcgtgatca cgcgcgagct gccttccttc gccgtattga tcgaatcgag tcccgcaccg
     7561 atccagttga acgggcggaa cagcgtgtag tcgaggccgt cctgctggcc gtacgcgtgg
     7621 atcacgcggt ccatcagctg cttcgcacac gcgtagatcc agcgcggctt gtcgatggga
     7681 ccgagcacca ggtccgagcg cgccgggtcg aattcggcat cgctgcacat cccgtacacc
     7741 tcggacgtcg acggaaagac gaggcgcttg cgatggcgca ccgcggcgcg cacgatcggc
     7801 aggttcgctt cgaaatcgag ctcgaagacc cgcagcggct cgcgcacgta cgttgcgggc
     7861 gtggcgatcg cgacgagcgg cagcagcacg tcgcacttct tcacgtggta ctcgatccat
     7921 tcgcggttga tcgtgatgtc gccttcgaag aaatggaagc gccgctcgtc gagcagatcg
     7981 gcgatccgct cggtctgcat gtccatcccg tagatctccc agtcggtgct ggcgatgatc
     8041 ttcttcgaca ggtgatggcc gatgaacccg ttgacgccga ggatcaggat gcgtttcatg
     8101 caggaggtct ttggaatgga ggatcacggc cggttgttgg ttggatcgaa gccggtccgt
     8161 gcgaccctgg tttcattgta acttgcgtca atcgagcggc acgtcgacgg cgccgaagcg
     8221 ctgtcgaacc tccctcggcg ccgcaatgcg tccttcgact tcgagcacgt cgatcgcaag
     8281 cgtgccgccg ccgccgcagc gcgcgatcaa ccggtcgcgg gccgaaagcg ccggtgcagc
     8341 cgccggcgct tgcgcatcga gcacgcgggt cttgagcacg cgcgacggca cgccgccgat
     8401 cgacgtccgc gcaccggggt agggcggcgc gaccgcgcgc accaggttat ggatgtcgat
     8461 cgccggcctg ctccagtcga tgcggccgtc ctcgggcgtg cggccgccga agtacgagcc
     8521 gcgcgcgacg tcgttgcgaa tgcgcggcgc cgtgccggcg atgaggccag gcagtgctgc
     8581 atcgaggcag atctcggcgg caacggtcac cttgtcgaac acctcgcgtg ccgtatcgtc
     8641 gggcaggatc gggacggcct gctgcgcgac gatgtcgccc gcatcgggct tgccggccat
     8701 gtagtgcagc gtggcaccgg tgcggcgctc gccgtgcaac accgcccagt tgaccggcgc
     8761 gcggccgcgg tagaacggca gcagcgagcc atgcatgttg agcgcaccgc gccgtgcaag
     8821 cgcgaggagc ggcgggctca gcatccggcg gcagtagaag ctgaacagga aatcgggcgc
     8881 gagcgcggca atgcgatcga cgacgtccgg cgcattgacg tcggcgggcg cgatgcaggg
     8941 caagccgagc tcggcggcga cgtcggcgac gcgggcgaac cacggtgtct cggcgggatc
     9001 gtcggtgtgg gtgacgacga gcggcacgtc cacgccgtgc gcgcggagca cccgcaggca
     9061 gcggtcaccg acgttgtgat aggcaaagac gacggcgcag cgcccgtcgg gctcacgcgc
     9121 tgtactgccc cgcaacgggg cggaatccga gccttgcggc ggccctgcgg cttgcatacg
     9181 gctcagcgca gcgcccgcac gctctccgcg ggccggtgcg gcaggtcgac gtcttcgtgc
     9241 tcgcgctcga gcacggcgcg gatcacgtac cgcggccgtt cgcgcacctg ctggtagata
     9301 cggcccacgt attcgccgag gagcccgacg ccgaacatga tgcagccgat caggaagaac
     9361 gcgaggatgt cccggtccca cagcgccttg atgccttcga ggaatccgcc gatcaccgcg
     9421 cgctcgacga tgacgatcgc gtacaggatg accgacgtga tggcgacgat catgccaacg
     9481 accgagaaaa gctgcagcgg cacgagcgaa aagccggtca cgaggtcgaa gttgagccgc
     9541 atcagccgat acagcgagta tttcgattca ccggccgcgc gctcctcgtg cagcacgtcg
     9601 acttcggtgg ggtggtgggc gaacgtgtac gcgagtgcgg ggatgaacgt gctcacctcc
     9661 cgcgcggcgg cgaccgcatc gacgatgtcg cggctgtagc cgcgcagcat gcagccctgg
     9721 tcggtcatgc gaatgcgcgt gatgcgctcg cgcagccggt tcatcgcgcg cgacgccgcg
     9781 cggcgccacc acgcgtcctc gcgcgcgcgg cgcacgccgc cgacgtagtc ggcaccggcg
     9841 tccatcgccg cgaggagctt gccgatttcc tcgggcggat tctgcaggtc ggcgtcgagc
     9901 gtcacgaccc gttgcccgcg cacgcgctcg aagcccgcga ggatggcaag gtgctggccg
     9961 taattggcgt tgaagaggat cacgcgggtg acgtcgggac gcgcggtgaa ctgctcgcgc
    10021 aggagcgcgg ccgagcggtc gtggctgccg tcgttgacga agatcacttc gtacggcacg
    10081 cgaagcgcgt cgagcgcggg ataaaggcgc ccgaacaacg ccgccaaccc gtcctcctcg
    10141 ttgtagacgg gaatgaccac cgaaagctcg ggaagggtca tttcaatgcc tcggcagcag
    10201 atcggcacac gcggcgcaca cgcggtcgac atcggccgcg tccatcgccg cgtgcagcgg
    10261 cagcgaaacg gtgcgcaagg cgatgcgctc ggtgttgggc agatcgccgc gccgataacc
    10321 gaagcgccgt ccgatcgtcg acaggtgcag cgcctcgtac gacacgccgg tcgcgatggc
    10381 gcgggcggca agtgcgtcgc gaaaatcctt gcgcgagtct ttcatggcgt cgggcagcag
    10441 gatgcagaac atgttccacg actggccgtc gccgtcggcg ggacgcggcg gcagcacgca
    10501 tccgcggtcc gcaggaaagc gttcgaagta gcgcaaaacc agcgcgcgcc ggtgcgcgag
    10561 gaactgcggc aaccgcgcga gctgctcgac gccgatgcgc gcattcacgt cgggcaggtt
    10621 gaacttgccg ccgggaaacg caacgtcgcg cgtgccgtcg ggcaaccgct cgatgccgtg
    10681 gaagcgcagc acctcgacgc ggcgcgcctc ggcatcgtcg ttgcatacga gcgcgccacc
    10741 ttcgatcgag gtgatgttct tgttcggatg gaagctgaag gtgacgagat cgccgaacgc
    10801 gccgattgcg cggccgttga agtgcgagcc gatcacgagc gccgcgtcct cgatcacgcg
    10861 cacgccgtgc cgccgcgcaa gcgcataaag cgcgtccatg tcgacgagcg agccgggcca
    10921 gtgggtcgga atgatcgcgc gggtgcgcgg gccgattcgc gattccatct gcgcgagatc
    10981 gatgttgcgg gtgacgaggt cgcagtcgac gaacaccggc gtcgcgccct gcttcacgat
    11041 catgttgacg acggtgaaga agctttgcgc acaggtgatc acttcgtcgc cgggtccgat
    11101 gccgcagagc tgaagggcga cctccacggc cgcggtggcc gaggtcagca cgcgcaccgg
    11161 ccggccgtcg cagaattgcg acaacgcagt ctcgaagcgc tggacccaca cgccgctcgt
    11221 gatgtgcccc gaacgcagga cgtcgccgac gccggcgatc gtcgcctcgt cgatcagcgg
    11281 actcgcgaac ttcaggatat cggccatcaa ctgcgcgcca cgagatagac accggcgacg
    11341 atgaagccga tgccgagcca gcgctgcgcc gtcaccgcct caccgaacag ataatgggcg
    11401 gcgatggcat tgacgatgta gccgagcgaa agcatcgggt aggcgatcga caccggcacg
    11461 cgcgacaggc cgatgatcca cacgatgatg ctcacgccgt agagcgccgc gcccgcgatg
    11521 aaatagccct cgctcgccat ccgggtcagc gtcgccgccc agttgccggc gttgagcgtg
    11581 atgacgccga gacggttggt gccgcccttg agcagcaatt gcgccgcggc gttcagcagc
    11641 acgccggtga acaggaacag gaacgccgcg gtcgacatgt cagtcgggaa gggccgcccc
    11701 gaccgactga cccgccctcc ggggggcagc gagcggagcg agcgtggggg gatgtttcat
    11761 cctagcgtcg cgccaccagc acgcgccgcg agtcgctcgc gacgacccgc atcggcacgt
    11821 cttcctgcgc gagcgtcgcg tgcgtttggg gcgccatcag cgcgtaaccc tgcggcaggg
    11881 accgccagcg tgcgatccag tcggacacat gggcaatgcc gcggcccggc tcggcatcga
    11941 gcccgggtcc gagctcgtcg cgatactcga cgagcgtggt ggtgcggccg aggtagaagg
    12001 gcaacgtctg gtcatacatg cgaacctgga agaacggcgc gtccggatcg tacggaggat
    12061 tggctgcatt ttccagtgtc gtgacgagat cggcggccga gcgggtcgcg cggaacacgt
    12121 cggaacccga aaacgtcgcc tgcatcgcga ggatcgtcgc cagcgaaagg gcgacgatgc
    12181 cgagcgtgcg gccgcgctcg tcattgcggc ggaacgcgag atagccggca atgtagccgc
    12241 ccgcggcgat accgagcgcg agcttcaccc agtgaccgag tgcgtcgtag agcgctcgcg
    12301 gcgtccgcgg gtcggcgagc gcgtcggcga tgcgcggcca gccggcgagt gccacgagcc
    12361 acagcgcgag cgtcacggcg ccgagcacgg cggtgaatac ggcaagcgtg gcaacacgcg
    12421 ttcttgcaag ctgccggccg agcaccagcg ccgccgcagg gaacatcggc aggatgtacg
    12481 acgggagctt cgaaccggaa atgctgaaga agacgaagac gaaagccgcc cacacgagac
    12541 agaagcgcac ccaggcgaag cctcgcgcat cgacgacggc atcgcgccag ccgcggcgca
    12601 gccgccacag gaagaggccg gtccatggca ggaaccccac gacgaacatc ggcacgaaat
    12661 accaccaggc gccgatgcgg cggtgctcgg tcgtgaggaa gcgctcgaag tgctcgtgga
    12721 tgaagaagaa gcgggcgaac tcgggattgc gcagcgagac caggacgaac cagggggctg
    12781 cgagcacgag cagcaccgcg aggccggcgc gaagatgcag ccgcttccac ggcccgcgat
    12841 cacgcgtggc aagcgagtag acgatcagcg tcgcgaacgg gatgagcagc gcgacgaggc
    12901 ccttggtcag cacgccgccc gcggccgcgg cataggcgag cagcatccag cgccgcgcaa
    12961 ggatcggcga cgcggccgcg tgctgtgcgc ggaggaacgc gcaaagcgac agcgtcagcc
    13021 agaccgcaag cagcgcgtcg agcgtgacga ggtgcgagat cccgaacagc cacacgctgc
    13081 ccgcgagtgc caggcccgca taaaggccgg ccgccggcga cgcgatcacg gcacccgtgc
    13141 cggcgaccac ggcaacggtc gcgaatcccg caacggcgcc cgggagccgc gcggtccact
    13201 cgtcgagctc gaatgccttg aagctcgccg cggtgagcca gtactgcagc ggcggtttct
    13261 cgaagtactt gagcccgtcg agccgcggcg tcacccaatc gccgctggcg accatttcgc
    13321 gtgcgatctc cgcatagcgc ccttcgtcgg ggtgctgcag cttgcgcacg tcgaggttcg
    13381 cgaaccaggc aagcgcgaag acgagagcga gcgcaatcca gctgcggcgg gacagcgcgg
    13441 cgagggtcgg cgattcggga ggcaaataag aagcggcggc aaccggcagg acccgtcgat
    13501 cgggcttcgc attctagcgt gacgccgcgc cgcaccgttc gtgcggtcgt tgcacaccgg
    13561 tcgtgccggc gcatcaatac cggatggcga agtcggtgag cgacagcccg atcaggatcg
    13621 cgagccagaa gaatccggcc atggccgcga tgcgcagcat gtagtgcgcc gagcgcaggt
    13681 gcatgaagaa gaaggccacc agcagcgcct tcgcgaccgc gatggccagg ttgatcgcgg
    13741 aattcgccca cccgagcggt acgtaggcac tggcgagcgt gacgacgagc aggccgatga
    13801 gggccagcca gacgaggacg taggatcggg cgcgggcgac cggatgggca atcatgtgcg
    13861 cgagacgagg tagagcagcg gatagagaaa gatccacacg atgtcgacga ggtgccagta
    13921 gaggccgccc agctcgaccg gcgtgaagta atcggccgtg aaccggcgcc gcgccgttcc
    13981 gatggcgagc accgcgacga ctgcgatgcc gatgatcatg tgcaccgagt gcaggccggt
    14041 catgaagaag tagaggtaga aaaagttctc gacgccgccg gcatgcggac cttgatgcgc
    14101 gaaatcgagc acgggtacga gatggccggc gtagtcgagg ccgtactcgg tgaacttgat
    14161 cgcgaggaat gcgatgccga gcacggcggt ggcgcacagg aagccgagtg cggcgcgccg
    14221 caggccgagc ttggtcgcgc gcacggccag cgccatcatc aggctgctcg tcagcagaac
    14281 cgcggtgttg atgctgccga gcgtgacgtt cgtgagacgg ctcgcaatcg cgaatgcctg
    14341 cgggtcgtgg aggcgcgtgg cggtgtaggc ggcgaacagc gcaccgaaaa agaggatctc
    14401 ggtcgcgagg aacacccaca tgccgaggtc ggacgccgtg cgctgctgcg cggcgtcgtc
    14461 gaactggcgc gcgacgatga cgacgctagg catgcgtgcc atccgtccgt cccccggcgc
    14521 ccggccgttc gtctcgcgga ctcaccggtc ctccatcgcc gaatactggt acggcggcgc
    14581 cgtgacgatg gggattgccg cgaagttcgc gaccggtggc ggcgatgcgg tctgccattc
    14641 gagtcctgtc gcgcgccatg gattgggccc ggcgcgcgcg ccatgcttga gcgaccacaa
    14701 caggtagaag agcggcagca ggtagccgac cgccaggatc gacgcacccg ccgacgacag
    14761 cacgttcagc acctggaatt cgggtgggta ctcgtgatag cgcctcggca tgccgaggta
    14821 gccgagcagg aactgcggaa agaacgtgag attgaagccg aagaacacgg tgatcgcggc
    14881 gaagcgggcc cagcggtccg aatacatgcg tccggtgatc ttcggccacc agaagtggac
    14941 ggcaccgagg aacgccatga ccgagccgcc gaccatgatg tagtggaaat gcgcgatgac
    15001 gaaatacgtg tcgtgcagat gcacgtcgag cgcaagcgac gcgaggaaga gccccgtcat
    15061 gccgccgatc gtgaacaggc ccacgaagcc cagcaggtag agcatcgggg catcgaagcg
    15121 gatcgagccc ttgtagagcg tcgccgtcca gttgaacacc ttgatcgccg acgggatggc
    15181 gacgaggaac gtcaggatcg agaacaccat gccggcgtag accgactggc cgctcacgaa
    15241 catgtggtgg ccccagacga ggaagccgat gatcgcaatc gcgagcacgg cgaaggccat
    15301 cgagcggtac gcgaagatgt ccttccgcgc gtaacaggtg atgatctcgc tcgccacacc
    15361 catggccggc aggatcatca cgtacacggc cgggtgcgaa tagaaccaga acagatgctg
    15421 gaacagcagc ggatcgccgc cgagtgccgg gtcgaagacg ccgacgccgg cgaagcgctc
    15481 gatcgcgacc aggaacagcg tcatcgcgag cacgggcgtc gcgagcacga agatgatgct
    15541 cgtcgcgtaa atcgcccaga tgaaaagcgg cagccggaac caggtcatgc cgggtgcgcg
    15601 catcttgtgg atcgtcacga tgaagttgat gccggtcagg atcgtcgaga agccgacgat
    15661 gaacacgccg cacacggcgg cgatcacgtg cgtgttcgag aacatcgtcg agaatggcgt
    15721 gtagaacgtc cagccggtgt cgacgccgcc cgcgaccagc gcgccgaccg tgaacaggcc
    15781 gccggcgatg tacaggtacc agctcgcgag gttgagccgc gggaacgcga ggtcgcgtgc
    15841 gccgatcatc agcgggacga ggaaattgcc gaacgtcgac gggatcgacg gaatcaggaa
    15901 gaaccacacc atgatcacgc cgtgcatcgt gaagagcttg ttgtacgtct cggcgctgac
    15961 gaggtcgccc gccggcgtga tgagctccat ccggatcagc gtcgccgccg cgccgccgac
    16021 gaaaaagaag agcgtgatcg agatcgcata caggatcgcg atgcgcttgt ggtcgacgct
    16081 cgcgatccac gaccagaggc tgaagccttc ctccaggtag cttcgcggca gcggcagggt
    16141 cacggtgctc atcgatcgtc ctcgcaatct cgcgcatgcg cgctcaacgc accgttcccg
    16201 gctcggcgcc gcgcagcgac ttgatgtact cgacgatttc gagcaggtcg tcctcggcga
    16261 tttcgccctg gaacgacggc atgatcggct cgtaaccggc gacgacgtcc ttgcgcggca
    16321 gcatcaccga gtcgtggatg tagcgctcgt cggcgaccac gctcgacccg tcggacagct
    16381 gcacgcgccg gccgtacaat ccctcgagct tcggcgcgtg caccgatgcg ctggcgccgt
    16441 gacagccgct gcatccgtac tggcgaaagc gcgccgcgcc ctcggcggcc atcgattgcg
    16501 acgcattgcc gctcgtgagc caccgctcga aggccgcggg ctgcatcacg acgacgtggc
    16561 cgatcattcg cgcatggtcg gtgccgcaat attgcgtgca gaacacgtgg tagtcgccgg
    16621 gcttgtcggc ggtgaaccac aggatcgtgt agcggccggg cagcacgtcc tgcttgatac
    16681 gaaagaccgg catgaaaaag ctgtgaatga cgtcctgcga cgtcatcacg agcttgatcg
    16741 gccgcccggc cggcacgtgc agctcgtcga tctcgcgctg gccgcccgta tgctcgagct
    16801 tccacatcca ctgctttccc accgcgtaca cctcggtcgc gtcggccggc ggcgcggcgt
    16861 gcgcgtagta gaggccggcc gcccagaagc ccatgccgag gaacaggatc agcggcgcgc
    16921 cgatccatgc gatctcgatg cgatggctct cgcggcgcaa cgtccggcgc gacggcatgt
    16981 cgcgatcggc gcgcgagccg tggcggtatt tcaccgagaa gtagacgatc aggaagaaaa
    17041 ccgccacggt gacgacaccg gccagcgcga gccacgcgta gaacaggtga tcgacgcgcg
    17101 tggcgagcaa cgaatcggcc ggcggatgca gcgggaaatc ccacatggcg ggtcagcgca
    17161 aggccgtgcg ccggcgcatc gcgacgaaga gcgcgagacc cgcgacgagc aacgcgacgc
    17221 tgccgatccg cagcgcttgc atcaccagcg cgctgtaagg tcccgccggc gcaaagtgga
    17281 agcacaccag cagcaggcgc tcgaccggcg acccgatctc gtcggcggcc gcggcatcca
    17341 cggcatcgtg cagcgcggcc ggcgtgaagt cgaagccgaa gaagtagtgc gcgatgctcc
    17401 cctgcggcgt cagcagcacg atcccggccg gatgcgcgta ctggtgcgtg gcatcgtcgt
    17461 aggcatagcg aaagccggcc gcgtgcgtga gtgccgcgat gtccgccgcg cttccagtca
    17521 gcaggtgcca cgcgccggcg tcgacgttcg cctgcgcgag gtagcgacgc ttcatgcgcc
    17581 gtgcgtcggc cggcgcatcg cgcgcatcga tgctggcgac gagcacccgg tagctcccgt
    17641 gcgcaagccc gctgcgacca agcgccgccg cgacgttgcc gaccacggtc ggacacagcg
    17701 tcgtgcagcc ataccacgcg aacacgagca cgatcggcgt cgcgccgaag taatcgccga
    17761 gccgcacgtt gcggtccgct tcgtcgcgga acgcgagcgc cgccggtagc tgcgccgaca
    17821 gccgttgctc gaagcccgca cgctgcgaag catcggccgg cgtgagatgc gcaagcgcgc
    17881 ccgttgcgaa cgcgagcgtg cacaggaagg cgcagcaggc gcgcgcgatc atttttgcct
    17941 cgcggcacca cgggcgatca gcagcgccat ggcgcgctcg atcggaatgc gcacgatgcc
    18001 cttgtcgcgg tcgatccatc ggtactcgtt cagcatcgcg cgcttctgcg cgcgcagcgt
    18061 ggcgaggtcg agtgccgggg cgctttgcag ccgcggcgac ggcatcgcgg tcggcggcgc
    18121 gtcggtcggc gacagcgggc ggccggtttc gtgatggaag aacgcgacga cgccggaagc
    18181 aatgaatccg gctgcgacga tcgtcgccgc gagtgcggcg gccgtctgcc agacgcgacg
    18241 gccgtcgacg tcggccgtct cgtgggcggc gccgcgcagc ggcggtgcgt tcacgcgaac
    18301 gcggccctgc gcctcagcca tggtgcgtcg cctcgctgga aacgggcgcc gtcacgtcgg
    18361 gcgccccgcg tgcggtgcgc acgaacacgg cgagccagag gccgccaagg gcggcgatcg
    18421 ccgcgacatc gctccaggtc acactgatgc cgctcggccg gtgtgcggga acgaccagcc
    18481 agtacgaatc gacgacgtgt gcgaaaagca gcagcgcggc gagcgcgccg agcgcgcgcg
    18541 gcacccgttt ggcgcggcgc gacagcaata cgaggaacgg cagcgcgaag tggatcgcga
    18601 ccagaagcag cgcgacgtcg tgccagctcg tcgtcacccg cggcaggtac cacgcgattt
    18661 cgttcggcag gtcttcagcc cacgtgatca ggtattgcgt gaacgcgagg taggcccagg
    18721 tcagcacgaa catcagcagc aggttgccga gatcctggaa cagtcccggc acgtcgccgg
    18781 ggagccggct cgactcgggg cccgtcgacg caaggttggc ctggctgccg acggcgtcgc
    18841 gcaaccgcca cgccgcgcag acgatcgcga aggaaaacgc gcacatcgtc tgcccgatgc
    18901 cgacgaggaa gccgaacgtc gtcgagaacc agcgcggcga cagcgacatg atccagtcga
    18961 cgccggcgaa cgtcacggtc cccgcataca gcagcaggcc gatggcggcg atcgcgcgca
    19021 ggcgctcctt gtcgcgcagc gtcgcgccgg catggcgcgc aaacgcccac ttgcgcaaca
    19081 ggtggccgag ggcgagccag atcacgaagt agattgcgtt gcgcagcagg aagaagtcga
    19141 ccgacagata ccagcgcttg tcctgaagca gcgggtcggc ggcaacggca tcgggtcgcg
    19201 cccacgcgta catgtcgcga agaccgaaca gcagcggcac gaccagcagg agcgacagcg
    19261 gcagcaaccg cgccgccgcg tcgagcgggc cgcggatgac ctcgccccag ccgccgccgg
    19321 tcaggtcgtg cacggcgagc atcgcaatgc tgccgagcgg aatgccgagc aggaaccacc
    19381 agacgaacag atacgactgg aacgcggtcg cgcgatcggc gtacgccgcg gcagcgaagc
    19441 cgatcgcgcc ggccgcgccg acgatccacg caacgccttc ggcgtgccga acaggttcgg
    19501 cgcgaaggct cacttcgatc ctttcgcgag ctcggcgcgc cgctcgggcg gcacgtcgcc
    19561 gatgcgcgca tcctgcgcga gctgcagcgc gcgaatgtag gcgacgatcg cccagcggtc
    19621 gtgcggggcg acgcggtcgg cgtacgagta catcatgccg tagccgttcg tgatcacgct
    19681 gtagaaatgc gcgtcggggg cgttgcgcag ccgatcggtg tgatacgacg gcgggctcgg
    19741 aaagccgcgg cgcacgatca tgccgtcgcc gtcgcctgcg acgctgtggc acggcgagca
    19801 gtagatgtcg aagcgctcgc ggccgcgctc gagcgtcggc cggtccatcg gcagcggatt
    19861 cgccgcgagc agcggctgcg ggctgcccga cgtcagagcg gccttgacgc ggccccggtc
    19921 gtccaccgga tacgtcggcg cttcgctggc cgccagcggc agcgtgccga ggcgcccgct
    19981 cgacgtgccc gcggtggcgc cgccgctgta cgcgagcgtg ttcggaacat cgggccgcga
    20041 cgcgcggcca tcggcccaca gcgcgctggc gccgagcggc ttgtacttcg gctggttgta
    20101 catgtcctgc atcgccttct cgcaaccgga cagcgcgagc gccagcgcga ccggtgccag
    20161 cgcggtccgg gtcagcgtga cggcgacaag acgcgttgcg ggccggcgca tgttcacgag
    20221 tcggaggcca cttcggtgac gcgcaacggg tcgagcgact cgaggaagcg ctgcgtcgcc
    20281 gcacgctcga agcgcgggtc ggccgcctcg acgcacagga agaaacggtt ccggctcgcg
    20341 aggtcgaagt cgtccgcgtt gaagatcggg tgatgcagtt tcggcaggcc attcaggatc
    20401 aggaagccga agaacgcggc gagagccgcg ccgagcaccg tgcattcgaa cgtcgccggg
    20461 atgaacgcgg gccagctatg cagcggcctg ccgcccgcgt tgatcgggta gtcgatcacg
    20521 gccgaatacc actggaggaa atagatgcct acgccgccgg cgatgccgcc gagcagcgtg
    20581 atgagcgcga cgcgatcgcg gcgaaagccc agtgcgtcgg cgagcccggg caccgagaat
    20641 ggcgcgtacg cgtcgacgtt ccggtagccc gccgcggtcg cgcgcgcggt tgcgtcgacg
    20701 agcgcgtcgc cgtcgcggaa ctcggcgagg agaccgtaca gctctcgatc cgcgcttgca
    20761 tgatcccccc acgcttgcgg cttcgccgct gcgctgcccc cggagggggc gcaattcgcg
    20821 cctcgaggcg gctcggcggc gctcatgttt cgccctcccg cttctccagc acgagcttgc
    20881 gcatttcgga aatggagatc gccggcagga agcgcacgaa catcaggaac agccacccga
    20941 acgcgccgag cgagcctgcc agcgtcgccc agtcccagaa cgtcgggtaa tagatatccc
    21001 agctcgacgg caggaaatcg cggtgcaggc tcgtcacgat gatcatgaag cgctcgagcc
    21061 acatgccgat gttgacgacg atcgagatcg cgaacagcgt gagcgtcgac aggcgcacgc
    21121 gcttccacca catcgcctgc ggcacgagca cgttgcagaa gacgacggtc cagtagatcg
    21181 gcgcgtacgg acccgtcatc cggttgacga tcatgtattt ctcgaagcgg tcgccgctgt
    21241 agaacgccat gaagatctcg accacgtagc tgtacgcgac gagcagcccg cagacgagca
    21301 tcaccttggc gatgttgtcg aggtggcgcg ccgtgatgaa atccttgagg ccgaacaccg
    21361 cgcgcagcgg aatcgagatg gtcagcgcca tcgcaaagcc cgagaacaat gcgccggcga
    21421 cgaaatacgg cgggaagatc gtcgaatgcc agcccggggt gttgccgatc gagaagtcga
    21481 gcgacacgac gctgtgcacg gagatcacga gcggcgtcgc gaggccggcc atcagcaggt
    21541 acgcggtgtc gaagcggcgc cattgccgcg cttcgccccg ccagccgagc gcgaacagtc
    21601 cgtacacgat ctgcttgacg cgatggtcgg tgcgatcgcg cagcgtcgcg agatcgggga
    21661 tcaaccccat gtaccagaac aggatcgaca cgacgatgta cgtgccgatc gcgaagatgt
    21721 cccagaccag cgggctgcgc cattgcggcc acacgttcat gatgtccggg taggggacga
    21781 gccagtagaa gaaccacggc cggccgagat gcaggatcgg catcacgccc gccatcgcaa
    21841 cggcgaagat cgtcatcgcc tcggcgaacc ggttgaccga cgtgcgccac ttctggcgca
    21901 gcagcagcag gaaggcggag atgaacgtgc cggcgtggcc gatgcccacc caccagacga
    21961 agttggtgat ggcgaatgcc catgcgacgg ggatgtcgac gccccagatg cccacgcccg
    22021 tggtgaacag atagacgatc gcgacgacga acagcacggt cagtgcggtg cacagcgcga
    22081 gaccggcgta gaagccccaa tgcgtcgggc gcgtcaggac gatgtcgctg atcttgtcgg
    22141 aaatgctcgc gtacgaatac ctgccgtgga gcacctcgag gcgcgactcg tccatgccgt
    22201 tgccggtggc ggtgatggcg ggtggcacgt caggactcgc cgaggtcggg gttgggatta
    22261 cgcagcttcg cgagatagct cgtccgcggc tcggtgttga gctcggcgag gagcgcgtaa
    22321 tcgagcggcg acgacttgcg cgcattcacc gcgctcgccg gatcgttgag gtcgccgaac
    22381 gtgatcgcct gcgtcgggca tgccgcctgg cacgcggtca ccacctcgcc gtcgcggatg
    22441 cgccggtcct gcttgtccgc ctcgatgcga ccgttcgcga tgcgctgcag gcagtagttg
    22501 catttctcca tcacgccgcg catgcgcacg gtgacctccg gattgcgctg gcccttgagg
    22561 ctctcggtgt gctcgtcgct gtattgcagg aagttgaacc ggcgcacctt gtatgggcag
    22621 ttgttcgagc agaatcgcgt gccgacgcag cggttgtaga cctgcacgtt gatgccttcg
    22681 gagtcgtgca ccgaggcctc cacggggcac acgagctcgc acggggcgcg ctggcactgc
    22741 atgcacggca ccggctggaa gtgcgtgcgc ggcgcattcg gcgcgccgtc gtaataacgg
    22801 tcgacgcgga tccagtgcat ctcgcggccg agctcgacct gcgccttgcc gacggtcgga
    22861 atgttgttct ccgcctggca ggcgatcgtg caggcgttgc agccgatgca ggcgccgaga
    22921 tcgatcgtca tcccccactt gtagccgtcg taggcaaacg gctcgtagag cgtcccgttc
    22981 gggcgctgcg gcgcgcccgc ggcgcgcggg tcgcgccgga aatcggcgag cgtcgcgacg
    23041 cgcacgatgt cgcggccttc catcgaatga tggtgctgcg tcgttgcgag cttgtagcgg
    23101 cggccggtct tgcgtagcga gaggccggct gcgaaccacg cggcgtcggc gctgcgcagc
    23161 gcgtacgcat cgaagccgtg agcgtcgccg acgcggccgg cgcggctgcg gccgtagccc
    23221 aggctgaccg tcacggcgtc ctccggatga ccgggcagga cccacacggg tccgctgacg
    23281 cttgcaccgg cgcggcgaag ctcgacgacg tcgtcgttgg caaggccgag gcgcgcggcg
    23341 agtgcggggg ccaccagcac cgcgttgtcc caggtgatct tcgtgagcgg ccgcggcagc
    23401 tcctgcagcc agggattgtt cgcgtaacgg ccatcgtgca cggacgcgtc gggcacgaac
    23461 tgcaggtcga gcgaaccttc gccggcgttc ggtgccgcct gcgcggtcgt gccggcgagc
    23521 gcttcactgc gcagcgcgac gctgcgttcg ggcaatgcgc tgccggcgac gacaccgttg
    23581 cggagcgcgg tctgccaggc gatgtcggga tcgggaccga gcttcggctg ccagaactcg
    23641 cgcacgatcg cgtagcccac gcgcggcgtg ccgtcggcga gcatcgacac gacctcgtgc
    23701 gccgaacgtc catcgtagag cggcgcgatc agcggctgga tgatcgtcac cgtgccgtcg
    23761 tacgcacgcg catcggacca cgcttcgagg aagtgcgccg ccggaagatg ccattcgcag
    23821 tgcgccgacg tctcgtcctc gtggagcccg aggcgcacgc ccatcgcgac gttgccgaga
    23881 ttttcggcga agcgcagctc gcgcggcgcg tcgtagaccg gattgccgcc gacgatcacg
    23941 agcgtgtcga cgcgatgcgc accgatatcg gcgacaagcg ctgcaagcga ctgcagctga
    24001 ttgaccgggt tggcgaccgc gggctcggca tggctcacgg tgacgccggc gttgccgagg
    24061 tacgcgttca tcgcgtgcgc caaggcgtgc acctgcggcg gctggcgctc gccggcgacg
    24121 acgagcgatg cgccgcgatg cgtatcgaga tcgtgcgcgc acgcggcgag ccatgcgtcg
    24181 ggagccccgg cgacgctgtc gcccgcggcc cccggcacgc cgaggcgttt cgcaatccag
    24241 cgtgcggccg cttcgatgcg gcccccgcgc agcgccagcg cgtgatcggc gtaggcactc
    24301 gtgaggctcg gcgtggccgc gatcgcgtag agccggttcg actcgccgat ggcgccgtgc
    24361 gtcgcatggt ggctgacgaa gtcgcgcgcc gcgcgcacgc ggtcaggacc ggtgccgagg
    24421 aaatcggcgt cgagagagag caccaccgcc gcgcggtcga aacgatggcg gacgtcgagc
    24481 ggcgtgccga acgcgaggcg cgcgccgtcg taggcattgt cgcggttgac cggctcgtac
    24541 tggtgccatt gcgccctcgg ataacgcgcg agcagcgcct gcagttgcgc ggcgagtgtc
    24601 ggcgacgtga cggtctcggt gaggatgcga agtccgtcgc cttgccgcgc tccgaggtcg
    24661 cgcaggcggc gcgcaagcgc tgcggcaaac ccatcccacg tgctgaccac gccgcggtgt
    24721 gtcaccgtct gcgatcgatc gggatcccag agatcgagca cggacgcctg cgcgaagacg
    24781 tcggtgctgc cgaggcttgc cggatgcagc ggattgccct cgaccttcgt cggccggccc
    24841 atggtgctct cgacgagcac gcccattgcg aagccgccca cggcattggc ggtggcgtag
    24901 aagcgcggtt cgcctgccac ctgccccagc ggcgcgttca cgtacggcag gatcctttcc
    24961 tgcggttcgc gcgtgcaccc tgcgagaccg gcgagcgcca gcgacgccga catcagcttc
    25021 aggaattcgc gccggtcgac gcggtagagg gcgccccacg acgggtgctc gtgcgccagg
    25081 cgttccgcga tggcgtcgcc gtccgtgagc tcgtcgaggc ttcgccacat caccgtcgtt
    25141 gctcctgcgc gtgcgtcatc gatggcacgt cgagcaatcg gtcagcgtgg atttgtggat
    25201 gtgatacgcg gcgacgaggc gggttccgag cgccatctga tcctcggccg gcttccagtc
    25261 catgtcgaac acgtgctcga cgggccgcag gtatttctcg ggcgtgcgat ggcagtcgag
    25321 gcaccattgc atttgcagcg gcgcgacgcg ccacgtgagc ggcatccggt cgatgggtcc
    25381 gtggcaggtc gcgcagccga cacccttcgc gatgtggatc ccgtgattga aatagacgaa
    25441 gcccggcaga tcgtgaacgc gattccagac aagcggccgt ccgctcgcga gactttgcac
    25501 gaccggggcg aggatcggcg cctgcgtgaa aagctgcgaa tggcaggtca tgcacgtgtg
    25561 cgtgggcggg attcccgcaa acgccgattg ctcgaccgac gtgtggcaat agcggcaatc
    25621 gatgccgtcg tcgccgacgt gatgcttgtg gctgaaggca acgacctgct cgaccggcgt
    25681 gccgatcgcc gggtgcggtg cgatcaggat tcgccacgcg agcagcgcgg cgaacaccat
    25741 cccgagcacg gcgagcaagc cgagcttgag ggccaggacg agacgtggct ggaagacctg
    25801 cgccatcggc gctcaccgcg ggcggtgccg cgacgagcgc gatgcgccga tgctgcgcgc
    25861 ggcgttcgac gtgccgatga tggtttccag gaatggcaat gcccggcgga tcgcggcgta
    25921 cgcgacgcgt tcagcgatgt aatcgattgc acggtcgtgc atcacgcgtg ccaggtcaca
    25981 ctcccagcgc gccggcgttg attcgcaaag gcaatgtcgt cctccgccgg tgtgcggcga
    26041 cgtgctgcgc ggcccggctt tttccgattc aggaaatatt tacaacactc ggcgtgctaa
    26101 agtcgccgcc ctgcgcatcg atgcgcgcac atgccgcgcc gctgaccgaa ttgcccgcgc
    26161 tctccgtcgc tatcgccgcc gcccgcgcgg cggacctgcc gctcgtcgcg cgactcgcgc
    26221 acgagatctg gcaccggcac tacccgggca tccttcccgt cgcgcagatc gattacatgc
    26281 tcgcgcgagg ctatagccgc gaagcgctga ccgattacct gacccgcgac ggcgcgggac
    26341 tcgccctcgc gatgcacgcc gagacggccg ttggattcgt cggctggtat ccgctcgagc
    26401 cgcccggtac catgaagctc gacaagctgt acgtgctgcc cgagcaccac ggcacgggaa
    26461 tcggacgcgc gctgatcgac catgtgatca cggaagcgcg tgccgccgct tgccgtgcgg
    26521 tgacgctcaa cgtgaaccgc ggcaacgccg gatcgatccg cgcgtacgag cgatgcggct
    26581 tcgcgatcac cgaacgcggc gactttccga tcggcaacgg ctacgtgatg gaggatttca
    26641 tcatggtgcg gcaactctag cgtcgctatg gcaagtcttg ccctctacga caactacgcg
    26701 cggagcctgc gcgaattcag tccgctcgat ccgggtcgtc ccgttggcct ctatacatgc
    26761 ggtcccaccg tctacgacta tcagcacatc ggcaactacc ggacgttcct cttcgaggac
    26821 ctgctgaagc gcgtcctccg atggaacggc tatcgcgttc gccacgtgat gaacatcacc
    26881 gacgtcggcc atctcacctc cgacgccgac accggcgagg acaagatgga gaagggcgca
    26941 cggcgcacgg ggaggagcgc gtgggagatc gctgcgctct acacgcaggc gttcgtcgac
    27001 gacctccgcg cgctcaacat ccttccagcc gacgtgctgt cgcgtgccac cgaccacatt
    27061 gccgagcaga tcgcgttcat cgccgatctc gagcagcgcg ggtacaccta tcgcacgtcg
    27121 gacggcatct atttcgacac gagccgccag gccgactacg gctatcttgc gcgcctcgac
    27181 cgcgcaggcc tccaggccgg caggcgcgtg gacgtcggcg agaagcgcca cgccaccgat
    27241 ttcgcgctat ggaaattctc gccggcggac agcacgcgcc agatggaatg ggacagcccg
    27301 tggggaaaag gctttcccgg atggcatatc gagtgctcgg cgatggcgca gcgctacctc
    27361 ggcgactggt tcgatatcca ctgcggcggc gaggatcaca ttccggtgca ccacacgaac
    27421 gagatcgcgc agaccgaagc gcgcgccggc acgcgtctcg ccaacttctg gatgcacggc
    27481 tacttcctgc tcgcgaacga cgcgaagatg gcgaaatcgg ccggcgagtt cctgcgcatc
    27541 gccgcgctgg tcgaacgcgg ctacgacccg ctcgcgtttc gctacctgtg cctgaccggc
    27601 cattaccgca cgcagctcaa tttcacctgg gaagcgctcg acgcggcggc gaccgggctt
    27661 gcacggatgc gaagcgcgtt ccacgcgctg ccgttcgacg ccgccgcaac gattccgcaa
    27721 gatctgctcg atcgttttac cgcgctcgtg aacgacgatc tcaacgcgcc gcgcgcgctg
    27781 gcgctggcat gggagacgct gcgcggcgac ttgcgtttgg cggtgaagcg cgcagcgctt
    27841 gcgcgcttcg acgacgtctt cggtctcgga cttgcacagt ggcagccggc ggaggatccg
    27901 atccccgcgg cgatcgcagc actcggcgag gcgcgcgccg atgcacgcaa ggcgcgcaac
    27961 tgggccgagg ccgatcgtct gcgggcggcg ctgcaggagg ccggattcga cgtcgaggac
    28021 acgccggacg gctattcgct caaacggaag taacgtacgc ctgcgtcagt acatgcgctc
    28081 gatgcgtccc gaatagagca ccggcccggt gggcttgccg gtgggcgatc cgcccggcgg
    28141 ctcgaggctg accgcgagcg tggagacgtt ggaaagcgtc tcgtcgaccg gattgtcgag
    28201 cggcacgcgg acgacgtcgc ccgcgggaat gacgccgagc gcgtggggtg caccggactg
    28261 cggcagcgcc catagctcgt acgccttgtc gggtccaggc ttggcgttgc tcaccgtctt
    28321 cagcgtcagg tagcgctcgc cgcgcgtggc cgtggcaatc agccccacct tcgcatcggc
    28381 atccgacagc acgacgacga gcggggccgg aacgatgacc ggtgcgcgcc gccactcgcc
    28441 gatgccgatc gcgatcaccc cggcgagact cgcgatggcg agcgtgcgcc agaatgcgag
    28501 gcgctgccac cacgcggtct cgcgcttcga tgtgccctcg ggatcgagcc cgagccgcgc
    28561 gacgatgttg cgccacgcgc gcggcggggg cgtcaccggc ggcacctggt cggcgagcac
    28621 cgccacgcgg cgttcccatt cggacagcgc agcggcgacg acgcgatcgc gccgcgaaat
    28681 cgcgatgaga cgccggcgca cgcgcggcgg tgacgtgcca agcgcgaact ccgccgcgag
    28741 ccggtcgagc cgctcgcgcc ggtcggagcg cgacaggttc attcggccgc tcccgccgca
    28801 tcgaggcagc ggcgcagcgt ggcgagaccc cggcgcaccc acgacttgac cgtgccgagc
    28861 ggctcgcgca gatgcgcggc cagttcgcga tgcgacagtc cgcggaagaa agcgagcgcg
    28921 atcgcctgtc gcggccccgg ttcgagcccg tcgatgcaac cgcgaagcgc gcgcgcgtcg
    28981 gccgcggcga gcagcagatc gaccggggtc ggcccgtccg acggcggctc ccaggcaggc
    29041 gaatcgtcgt cgtcgcgcgg cgtcagcgtc tcggtgtcca cttcgcgccg gcgcagccag
    29101 tcgaggcagc ggttgcgcac gatcgacgtg agccaggtca gcggctggct tttcgcggcg
    29161 tcgtacgtgc ccgcgtggtg ccacacgctc acgaacgctt cctgcagcat ttcctcggcg
    29221 atgggcccat cgcgcatgat acggacggcg accgcataca gatgcgcgct cgcaagaccg
    29281 tacagctcgg cgaatgcgcg ctggtccccc agggcgatgc gcgccagcag ggcggcgagc
    29341 ttctcgcttc gcgcgtttgg gccgggttgc gggtcgacac ccgtcatgcg ttcgcgccgc
    29401 gcatgcggtc cttttgccac agcacatccg gcgcgccggc cgcgcgattc agcacgcgcg
    29461 cgagcacgaa caagagatct gacagccggt tcaggtagat gcggctcgac gtcgagacgc
    29521 tgtcgccggc cgcaagcgca atcagcgtgc gttcggcgcg ccggcacacc gtccgcgcga
    29581 cgtgcgcgag cgccgcggcg cgcgtgccgc ccggcaggat gaattccttc agcatcggca
    29641 gattcgcgtt gtaatgctcg acagccgcct cgatgcgcgc gatatgcgcc ggcgtcacgg
    29701 ccgcgtggcc gggaatcgac aattcgccgc caagatcgaa aaggtcgtgc tggatgtcgg
    29761 tgagcaaggc gtgcacgtcc gccggcagcg actcggccag cgccacgccg atcgtgctgt
    29821 tgagctcgtc gaccgcgccg atcgcttcga tgcgcgggct gtccttcgcg acacgggagc
    29881 cgtcgccgag tcccgtggtg ccgctgtcgc cggtgcgcgt gacgatgcgg gagagccgat
    29941 ggcccatgag cgttcctggt ttgcgaactg cggttttcgt gtcgccgccc gatgcgcgcg
    30001 gcgctccggc cgacactagc cgaacggcgg cgccccggca agcgcctgcc ccggcgtcga
    30061 ttttgctacg atgcggtcac gaccggcacc acgacaggcg cgcacatgtc gacgcagcga
    30121 atgtacgaaa cgcatctcga ccgccgcccg gcgaacttcg cgccgctcac gccattgtcg
    30181 ttcatcgcac gcaccgccgc catctggccg catcgcacgg cggtcgtgca cgggcctcga
    30241 cgatacacgt gggccgggac gtacgcccgc gcgcggcggc tcgcatcggc cctcgcgcac
    30301 cgcggcgtcg gcgtcggcga cacggtggcg gccatgctct cgaacacgcc cgaaatgatc
    30361 gaatgccatt tcggcgtgcc gatgaccggc ggcgtcctca acacgctcaa cacacggctc
    30421 gacgcggagg cgctcgcgtt catgctcgag catggcgagg cgaaggtgct gatcaccgac
    30481 accgagttcg ccccggcgat cggtgcagcg cttgcgcggc tgcgcacgaa gccgctggtg
    30541 atcgacgtcg tcgatgcgct cggccccggc ggcgatctca ttggcgaatc ggattacgag
    30601 acgttcattg cgcacggcga cgccgacttt gcctggcagc cgcccgccga cgagtgggac
    30661 gcgatc
//
LOCUS       DASSVX010000288         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_495681, whole genome shotgun sequence.
ACCESSION   DASSVX010000288 DASSVX010000000
VERSION     DASSVX010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495681"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..388)
                     /locus_tag="VFO53_12470"
     CDS             complement(53..388)
                     /locus_tag="VFO53_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET8877136.1"
                     /translation="MASNSAAGYAASELSRGDVDALQGPVVLDFGTPWCGVCRRTAPL
                     VDDALARHPDVRHLRIEDGPGRRLGRSFRVKLWPTLVFLRDGGEVDRLVRPHEAEAID
                     RALARIDPR"
     gene            403..531
                     /locus_tag="VFO53_12475"
     CDS             403..531
                     /locus_tag="VFO53_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877137.1"
                     /translation="MRPRGFRPEPAGGQFVVAPDDLSGTPGWAKPIVAAALAADHA"
     gene            complement(577..1524)
                     /locus_tag="VFO53_12480"
     CDS             complement(577..1524)
                     /locus_tag="VFO53_12480"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8877138.1"
                     /translation="MDLPPALRSIRDFYFKDALAAPADVTHVHRPGLVRDYPPFSLEQ
                     LRRHLDDPRLDLNYLNLFQQGKTVDLADARLYKVVQRRKIAFVDRRVLQRHLASGAAC
                     VLEGLDILEPQINLLASVLDRGHTATFCNATAFFSQKGNEAYRGHVDTDDVLVIHVAG
                     EKRWRLHRRQKPRRTQLNELTESQMGAPDAEVVMRPGDVMFLRSYTPHRVETLSECSL
                     HVSFDLCDRQPSIEMALQLLLAHYDRDSALPLTPTGRTLDKLFEHAQGDAYAADLDAM
                     LARDKAGHAEFRRILDARVTHLDRFVEEERRHDLRSAAR"
     gene            1665..1973
                     /locus_tag="VFO53_12485"
     CDS             1665..1973
                     /locus_tag="VFO53_12485"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877139.1"
                     /translation="MNARKTLLALLMAAGTGATLTPAVASADIYVRVAPPPPRYEAVP
                     VVQPGWVWIPGYWNWNGRHYVWVNGHRVHARHGRHWVPDRWAEHRGHWRREHGHWDRD
                     "
BASE COUNT          338 a          666 c          727 g          337 t
ORIGIN      
        1 gccggatcga cagcgccgcg aacgatttcg cccgttgttc ccaagcgcgc aatcaacgcg
       61 gatcgatccg cgcgagagcc ctgtcgatcg cctccgcttc gtgcggccgg acgagccggt
      121 cgacttcgcc gccgtcgcgg aggaaaacga gcgtgggcca gagcttcacc cgaaacgacc
      181 gaccgagccg gcggcccggc ccgtcctcga tgcgcaggtg gcgcacgtcc gggtggcgcg
      241 cgagcgcgtc atcgacgagc ggcgccgtgc gtcggcagac gccgcaccac ggcgtgccga
      301 aatcgaggac gaccggtccc tgcagcgcat cgacgtcgcc tcgggacagc tcgctcgcgg
      361 cataaccggc cgccgagttc gaagccatcg cgataagacc cggtgcgacc gcgggggttt
      421 cgaccggagc cggcgggtgg acaattcgtc gtcgcgccgg acgacctgtc gggaacgccc
      481 ggctgggcga aaccgatcgt ggccgcggcg ctcgccgccg atcacgcgta gaccgtgggg
      541 catgccggtt ggcatgcaat ggatcctgcg agcgcgttat cgcgcggccg accgcagatc
      601 gtgtcgccgc tcctcttcga caaagcggtc gagatgcgtg acacgcgcat ccaggatgcg
      661 ccggaattcg gcatgtccgg ccttgtcgcg cgcgagcatc gcgtcgagat ctgccgcgta
      721 cgcgtcaccc tgtgcatgct cgaaaagctt gtcgagcgtg cgcccggtcg gcgtgagcgg
      781 cagcgccgag tcgcggtcgt agtgcgcgag cagcagttgc agcgccatct cgatgctcgg
      841 ctggcgatcg cagaggtcga acgacacgtg cagcgagcat tcggacagcg tctcgacgcg
      901 atgcggcgtg tagctgcgca ggaacatcac gtcgccgggc cgcatgacga cctcggcatc
      961 gggcgcgccc atttgcgatt ccgtcagctc gttgagctgc gtccggcgcg gcttctgccg
     1021 ccgatgcagg cgccagcgct tttcgccggc gacgtgaatc acgagcacgt cgtcggtgtc
     1081 gacatgtccg cgatacgcct cgttgccctt ttgcgagaag aacgcggtcg cgttgcagaa
     1141 cgtggccgtg tggccacgat ccagcaccga cgcgagcagg ttgatctgcg gctcgaggat
     1201 gtcgaggcct tcgaggacgc acgcggcgcc gcttgcgaga tggcgctgca gcacgcgccg
     1261 gtcgacgaac gcgatcttgc ggcgctgcac gaccttgtac agccgcgcat cggcgaggtc
     1321 gaccgtcttc ccttgctgaa acaggttgag gtaattgagg tcgagtcggg gatcgtcgag
     1381 gtgccggcgc agttgctcga gcgagaaagg cgggtagtcg cggacgaggc ccggccgatg
     1441 gacgtgcgtg acgtccgccg gcgcggcgag cgcgtccttg aaatagaagt cgcggatgct
     1501 gcgaagggcc ggcggaaggt ccatgaatgc aatggatgaa cggcagatgc gatcctacag
     1561 catcgttcgc cgagtcctac agcaccatcg tcccagggcc gatccagcgc tcgcgcgcgt
     1621 catgcgatcg ttggagccgt aatcgttgaa gtgaggctcg atcgatgaat gcgaggaaga
     1681 cattgcttgc gctcctgatg gccgccggaa ccggcgcgac gttgacaccg gcggtcgcgt
     1741 cggccgacat ctacgttcgc gtagcgccgc cgccgccccg ttacgaagcg gtgccggtcg
     1801 ttcagcccgg ctgggtctgg attcccggtt actggaactg gaacggtcgt cattacgtct
     1861 gggtgaacgg gcatcgcgtg cacgcacggc atggtcgtca ctgggttccc gaccgctggg
     1921 ccgagcaccg cggtcactgg cgtcgggagc acggccattg ggatcgtgac tgatcgacga
     1981 acgcagcaat gaccaacgag cggcgcgagt tcctcgcgcc gttttcgtgc acggtccggc
     2041 ggccatgcgg gcgccggtat gatccccc
//
LOCUS       DASSVX010000289        14155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1645905, whole genome shotgun sequence.
ACCESSION   DASSVX010000289 DASSVX010000000
VERSION     DASSVX010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 14155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14155
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645905"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VFO53_12490"
     CDS             <1..755
                     /locus_tag="VFO53_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877140.1"
                     /translation="LAQTPLRPDKVEPFIADVAVARVAPPVTRDTFKGTAIDHALDGL
                     LFPTSSGQWTALIGLRSPIGGTIDAAAVRYAVHDAGNAVLLDVRTEVDRLYAGYLERA
                     QIMSAVGAAAIVVLLLLALRSPARVARVLAPLAAAVLVVAGMHALMGTRLSLLHLIGL
                     LLVVAIGSNYALFFDRMAHVRDASAARTLTSLALANVSTVASFGILSLSSIPVLNSIG
                     GTAALGALCALVFAAMLVTSDRANDRPGGPVA"
     gene            763..1368
                     /locus_tag="VFO53_12495"
     CDS             763..1368
                     /locus_tag="VFO53_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET8877141.1"
                     /translation="MLALLSGTFSEPEDFVREGFSAAVHDHGIAAELVMAEMRASWFA
                     DGSVVDRIREAVIVPAHERGRARIWLVGISLGGLAALCYAARHASEIEGILLISPYPA
                     TRPVLREMQDAGGIERWKPAIPPGGDLEREAWSWLLQSRDGRPRVHCYFASDDRFAPG
                     QRQMAEAVAPERVRELPGGHDWDAWRALWREFLCNTKSALR"
     gene            1341..2195
                     /locus_tag="VFO53_12500"
     CDS             1341..2195
                     /locus_tag="VFO53_12500"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET8877142.1"
                     /translation="MQHQVGASVEVDLDLPAADLRWRPTPFIAGSLGLHVTALAGLVV
                     APEAWTWAAAAIAANQSVLGLAGLAPRSAMLGPNLARLSDAAAARMEVALTFDDGPDP
                     DVTPSVLDILDARGVRATFFCIADAAVRNPALCREIARRGHAVENHSRRHLPMFAMLG
                     PRGIRNEIATAQAMLAELGGRPPRFFRPPAGLRNPLLDYVLHAMELRLVSWTRRGFDT
                     RRRDADKVAASLFRGLTAGDILLLHDGNAARTASGTPVVLDVLPRLLDRIQLLGLRPV
                     ALHEAVSP"
     gene            2293..3489
                     /locus_tag="VFO53_12505"
     CDS             2293..3489
                     /locus_tag="VFO53_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET8877143.1"
                     /translation="MKPVLLSAYTMTSCLGHGLAPTRAAFRAAASGLAPCHFGAVDLE
                     TWVGEIAGLDDITLPHALARFDCRNNRASELALAQDGFSAAVAQSAARHGARRAGVFI
                     GTSTAGILQTELAYRRRDPASGALPPDFDYRRTHNTYSAAEYVRARLALAGPCAAIST
                     ACSSSAKAFASAARMLEADMIDMAVVGGVDTLCLTTLCGFDSLQLLSRAPCRPYDIER
                     DGISIGESAAFFLLERAPREGSIALLGAGESSDAHHMSAPHPEGAGARRSMEVALASA
                     GLVPETIDYVNLHGTGTPSNDAAEDIAVHALFGDAVPVNSTKGMTGHTLGAAGAVEAI
                     VSVIAIEEGRVPGSAGTRTVDPALRARYEVHGSARNIARVLSNSFGFGGSNCSLVFGR
                     VDGIDR"
     gene            3486..4277
                     /locus_tag="VFO53_12510"
     CDS             3486..4277
                     /locus_tag="VFO53_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HET8877144.1"
                     /translation="MSLAARVEGVGLIGPGLAGWPRARDVLCGRAAYAPSPTSIPAPE
                     ALPAVERRRAGKCVRIALAAGLEAVAAAGREPRDMGAVFTSSTGDGENVHAICEMLAS
                     NDRLISPTRFHNSVHNAPAGYWGIATGSRRSADSLAAFDASFGAGVVEAMSRIAACPD
                     EAVLIIAYDAPYPEPLNATRPIPDAFAVALALASTRAATRGATITLETGAGVADVLDD
                     PQLERLRRAIPAARSLPLLRLLAQERNGEATLDYLEGLSLTLRRS"
     gene            4274..4705
                     /locus_tag="VFO53_12515"
     CDS             4274..4705
                     /locus_tag="VFO53_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HET8877145.1"
                     /translation="MMRAPVDRTWLLANLPHQDGMNLLDGIVAWDASSVCAIARNHRT
                     IDHPLRRCGELPAVCGIEYGAQAAAAHGALASRESPGAGLLASVRSVVMHVRRLDDVP
                     GDLDVFAERLGGNDDGVLYRFEVAAAGRLLVEGRVAVAFPR"
     gene            4702..5409
                     /gene="fabG"
                     /locus_tag="VFO53_12520"
     CDS             4702..5409
                     /gene="fabG"
                     /locus_tag="VFO53_12520"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HET8877146.1"
                     /translation="MRKRALVTGGSGAIGSAICRRLATDGLEVVVHANGNLESARRLA
                     ADIGGSAVAFDVVDAKASLAALELVLEAGPIQVLVNNAGVHDDAAFPGMQREQWSRVI
                     AVAIDGFYNVTHPLSLPMIRTRWGRIISITSVTAQIGNRGQTNYAAAKGALHAISRSL
                     ARELGSRGITVNCVSPGLIDTAMSAGAFDAGAIARLVPLQRAGTAVEVADAVGFLASD
                     AASYITGQILPVNGGMA"
     gene            complement(5390..6154)
                     /locus_tag="VFO53_12525"
     CDS             complement(5390..6154)
                     /locus_tag="VFO53_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET8877147.1"
                     /translation="MVERSATHLVLIPSYNPGPAVYETVRGARQFWNPVWVVVDGSTD
                     GTADVLRGLAKTDSGLRVLTLPQNQGKGAAVLHGIRAAAADGYTHALTMDSDAQHPAA
                     SIPEFMARSMAARGSLILGDPVFDASAPRIRVMGRKLSNGFANLETAGGIGDSLFGFR
                     VYPIADLAAVMRDSPWMRRFDFDPEAAVRLVWRGLPPVNVRAPVRYLKREEGGVSHFN
                     YYRDNALLTWMHTRLVLESLLRLPILLLRKAMPSRR"
     gene            complement(6147..7487)
                     /locus_tag="VFO53_12530"
     CDS             complement(6147..7487)
                     /locus_tag="VFO53_12530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8877148.1"
                     /translation="MNEDLRSAGPAQECDVVVIGGGPAGSTASTLLADRGYRVVLLEK
                     ETHPRFHIGESLLPANLPLFDKLGVADEIRAIGMEKWGATFVSPWHDRISGFEFADAL
                     DPSMPMAYQVRRSEFDEILFRRASRAVADAREGCRVREIDLGGADRMPEVTATDAQGT
                     ARRWRAKFIVDASGRDTFIGSRLELKRRNPDHNSSAMFGHFRNAKRDEGKAGGNIIIS
                     WFEHGWFWFIPLKDGITSVGAVVWPYYMKARDKPLRAYFLDTIALLPPLAARLADAEL
                     VAEPQATGNYSYEMRACQGENFILIGDAYAFIDPMFSSGVMLAMNSGFAGADVVDARL
                     SADKPAETKARERFDYVMRRGPKEFSWFIYRVTNPILRELFMFPSERGGMKKAILSVL
                     AGDLFGNTDIRPGLRRFRLVYYLFSLAHLRRALAGWRRRAFNIRDDSEMRLTRG"
     gene            7578..8291
                     /locus_tag="VFO53_12535"
     CDS             7578..8291
                     /locus_tag="VFO53_12535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8877149.1"
                     /translation="MQRPIRYLPRYFADERGRRDFLDGLFDRTARDYDFVERLLGLGS
                     GSRYRRDALRRAGLAPRMRLLDVATGTGLVAREALALGGAVIGLDPSAGMLAEARRLA
                     YPLVRGLGERLPFRDECFDFVSMGFALRHVADIDALFAEMRRVLVPGGTACVLEITRP
                     ANRLVAGPLRVFMTRVVPAVARVARRDGASLMQFYWDTIDACVPPEAVLASLARAGFP
                     SPARNVALGMFSEYVARKR"
     gene            complement(8363..9319)
                     /locus_tag="VFO53_12540"
     CDS             complement(8363..9319)
                     /locus_tag="VFO53_12540"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET8877150.1"
                     /translation="MTRAARTLRDGGLVAFPTETVYGLGADAGNAKAIARIFDAKRRP
                     SDHPLIVHVGDIDAAHRWATLSAQAIVLANAFWPGPLTLIVPRAAHVDDIVTGGQSSV
                     GLRVPSHPVAHALLKEFGGGIAAPSANRFGHVSPTTARHVADDLGDAVDLILDGGASG
                     IGIESTIIALTTDTPMLLRPGGIAAEAIEAALGCPLAQPDSSAPRASGTLASHYAPRT
                     PAHMVEQDVLRAELAQLEARDECVAVLARTAEAPEAFDGVWLMAPRDAADYARLLYAN
                     LRELDDAKADAILIESVPDGTAWAAIRDRLTRATTGVDDDSD"
     gene            9508..10698
                     /gene="metK"
                     /locus_tag="VFO53_12545"
     CDS             9508..10698
                     /gene="metK"
                     /locus_tag="VFO53_12545"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET8877151.1"
                     /translation="MSEFLFTSESVSEGHPDKVADQISDAVLDAILEHDPTGRVAAET
                     LVSTGLVVMAGEITTSANVDYGRVARDTIRRIGYNDAALRFDADGCAVMVCYGRQSPD
                     IAQGVDRASDDYLNQGAGDQGLMFGYACDETPTLMPFPLYYAHRLVQRQSEVRRDGRL
                     AWLRPDAKSQVTVRYVDGKPKSVDTVVLSTQHHPSMNERQKELAEAVIEEIVRPVFPK
                     DMLSGTRFMVNPTGRFEIGGPHGDAGVTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDR
                     SAAYAARYVAKNVVAAGLAKQCQVQLSYAIGVAKPINVTVYTEGTGRISDARIVELVN
                     AHFDLRPKGIIRMLDLLRPIYRKTAAYGHFGRDEPEFTWEATDKVEMLREAAGLREAA
                     VAAD"
     gene            10730..11839
                     /locus_tag="VFO53_12550"
     CDS             10730..11839
                     /locus_tag="VFO53_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HET8877152.1"
                     /translation="MFDPLRLRELKLPNRIVVSPMCQYSAIDGCATDWHFIHWGQLLM
                     SGAGLFMIEATAVTRDGRITPGCLGLYDDACEEALTRTLERARKQCPPIIVGMQLAHA
                     GRKGSSHVPWEGGQLIPLDAGGWIPVAPSALPHAPTERPPAALDAAGLAAIRDAFVDS
                     ARRAARAGIDALELHMAHGYLLHEFLSPLANVRTDAYGGSFERRIAFPLEVFDAVRAA
                     WPAARPLGVRLSCTDWVDGGWTLEESTEFARRLAARGSDWIDASSGGVSPAQKIPLGP
                     GYQVHLSREIRRATAGARTIAVGLITTPAQAQAIIASGDADLVAMARAFLWDPRWVWH
                     AAAELGAAVAPPPQYLRSAPRDAASVFTGARIGQR"
     gene            11936..12064
                     /locus_tag="VFO53_12555"
     CDS             11936..12064
                     /locus_tag="VFO53_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014111951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877153.1"
                     /translation="MAERTRTRRNTLERRCLPKTFKRMFVGAPKGLFKMLEKIKKA"
     gene            12250..12795
                     /locus_tag="VFO53_12560"
     CDS             12250..12795
                     /locus_tag="VFO53_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877154.1"
                     /translation="MPFAYYNRLTAEQKRIYRASDAIESIALPPLAAVGSDVGAVRTA
                     LLNDDARAAQRACQRIADALTTVCRVPPLRIRVLAQRPSDDYGELHGLYEPVEGRRRA
                     TISVWMRTAKKRQVVAFKSFLRTLCHELCHHFDYEFFAFEETFHTDGFYKRESSLANA
                     LLVSEARTTTPSILGSSVGDL"
     regulatory      12834..12928
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            13005..>14155
                     /locus_tag="VFO53_12565"
     CDS             13005..>14155
                     /locus_tag="VFO53_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET8877155.1"
                     /translation="MNAVNETGQFADYEVADLALADWGRKEIAIAETEMPGLMSIRDE
                     FAPSQPLRGARIAGSLHMTIQTAVLIETLQALGADVRWASCNIYSTQDHAAAAIAARG
                     TPVFAYKGETLERYWDLTHRIFEWPGGLAPNMILDDGGDATLLVHLGAQAEKDRSVIA
                     HPTSDEEKALFASIAVRLDKTPDFYSTIKRGIRGVTEETTTGVKRLYQMHREGRLGFP
                     AINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKIALVCGYGDVGKGSAQALRA
                     LSAQVWIAEIDPICALQAAMEGYRVVTVDDACDKVDIVVTATGNIDVITYAHMARMKN
                     NTIVCNIGHFDNEIDVESLKAYKWENIKPQVDHVIFDDGKRIILLAEGRL"
BASE COUNT         2278 a         4680 c         4767 g         2430 t
ORIGIN      
        1 cgctcgcgca aacgccgtta cggcccgaca aggtcgagcc cttcatagcg gatgttgcgg
       61 tggcacgcgt tgcgccgccc gtcacgcgcg atacgttcaa aggcaccgcc atcgatcacg
      121 cgctcgacgg cctgctgttc ccgacgagct ccggacaatg gaccgcgctc attggactgc
      181 gttcgccgat cggcgggacg atcgacgccg cggcagtgcg ctacgccgta cacgacgcag
      241 gcaacgcagt gctgctcgac gtcaggaccg aggtggatcg gctctacgcg gggtacctcg
      301 agcgggcgca gatcatgtcc gccgtcggag cggcggcgat cgtcgtgctg ctgcttttag
      361 cgcttcgctc acctgcccgc gtcgcgcgag ttctcgctcc gctggcggcg gcggtgctcg
      421 ttgtcgcggg aatgcacgcg ctgatgggaa cgcgcctctc gctgcttcat cttatcggcc
      481 tgctgctcgt cgttgccatt ggctccaact acgcgctttt cttcgaccgg atggcgcacg
      541 tccgcgacgc ttcggccgca cgcacgctca cttcgctggc gctcgcgaac gtcagcacgg
      601 tggcgagctt cggcatcctg tcgctatcgt cgatcccggt gctgaattca atcggaggga
      661 ccgccgcact cggcgcgctc tgcgccctcg tattcgcggc aatgttggta acatccgatc
      721 gtgcgaacga tcgtccaggc ggacccgtcg cgtagcgcgc cgatgctcgc gctgctttcg
      781 gggacgttca gcgagcccga agacttcgtt cgcgagggct tcagcgcggc ggtgcacgac
      841 cacggcatcg ctgcggaact cgtgatggcg gaaatgcgcg cgagctggtt cgccgacggc
      901 tcggttgtcg atcgcattcg cgaagcggtg atcgtccccg cgcacgaacg cggccgcgcg
      961 cgtatctggc tcgtcggcat ttcgctcggc ggtcttgcgg cgctttgtta cgccgcgcgc
     1021 catgcgagcg agatcgaagg catcctgctg atctcgccgt atccggcgac gcggccggtg
     1081 ctgcgcgaaa tgcaggacgc aggcggaatc gaacgttgga aacccgcgat tcccccgggg
     1141 ggcgatctcg agcgcgaggc gtggtcctgg ctcttgcaaa gccgcgatgg gcgtccgcgt
     1201 gtccactgct acttcgcttc ggacgaccgc ttcgcgcccg gccagcgaca gatggcggaa
     1261 gcggtggcgc ccgaacgggt tcgcgagctt cccggcggcc acgattggga cgcgtggcgt
     1321 gcgctgtgga gagaattcct gtgcaacacc aagtcggcgc ttcggtagag gtcgacctcg
     1381 atctgccggc cgccgacttg cggtggcggc cgacgccgtt catcgcgggc tccctcggct
     1441 tgcacgtaac ggcgctcgca ggtctcgtcg tcgcgcccga ggcatggact tgggccgccg
     1501 cggcgatcgc ggcgaatcag tccgtgctcg ggctcgcggg actggcgccg cgcagcgcga
     1561 tgctcggccc caatctcgcg cgtctgtccg acgcagcagc cgcgcgtatg gaagtcgcgc
     1621 tcacgttcga cgacgggccc gatcccgatg tgacgccgag cgtcctcgat atcctcgatg
     1681 cgcgcggcgt gcgcgccacg ttcttctgca tcgcagacgc cgccgtgcgc aatccggcac
     1741 tctgtcggga gatcgcgcgc cgcggtcacg ccgtcgagaa tcattcgcgg cgtcacctgc
     1801 cgatgttcgc gatgctcggc ccgcgcggaa ttcgcaacga gatcgcgacc gctcaggcga
     1861 tgcttgccga actcggcggc aggccgccgc gcttcttccg cccgcccgcc gggctgcgca
     1921 atccgctgct cgattacgtg ctgcacgcga tggagttgcg gctcgtcagc tggacgcggc
     1981 gtggattcga tacgcgcagg cgcgatgcgg acaaggtggc agcatcgctg ttccgcggcc
     2041 tcaccgctgg agacatcctg cttctgcacg acggcaacgc ggcgcgcacg gcctccggca
     2101 cgccggtcgt gctcgacgtg ctgccgcgcc tgctcgaccg tatccaattg ctcgggcttc
     2161 gaccggtcgc gctgcatgag gccgtgagtc cgtgagcgac gcgtgttgcc ggttgcccga
     2221 cgcagacgcg ggtgcgcgag gcgagccgga cgatcgtttc gcgcgtcgat tcgcgttcgg
     2281 ccagctcacg acgtgaagcc cgttctgctt tcggcctata cgatgacgag ctgtctcggg
     2341 cacgggctcg cgccgacacg cgcggccttt cgtgcggcgg cgagcgggct tgctccgtgc
     2401 cacttcggcg cggtcgatct tgaaacgtgg gtcggcgaga ttgccggtct cgatgacatc
     2461 acgttgcctc acgcgctcgc gcgtttcgat tgtcgtaaca atcgcgcgtc ggaacttgcc
     2521 cttgcgcagg acggattctc cgccgcggtg gcgcaatccg ccgcgcgcca cggggcccga
     2581 cgtgcgggtg tgttcatcgg cacgagcaca gccggcatcc tgcagaccga gctcgcttat
     2641 cgacgccggg atccggcgag cggtgcgctg ccgcccgatt tcgactatcg ccgcacgcac
     2701 aacacgtact ccgcggccga atacgtgcgg gcacgcttgg cactcgccgg cccttgcgcg
     2761 gcgatctcca ccgcgtgctc gtcgagcgcc aaggcgttcg cctcggcggc tcgcatgctc
     2821 gaagcggaca tgatcgacat ggcggtggtg ggtggcgtcg acacgctctg cctcacgacg
     2881 ctgtgcggct tcgattcgct gcagttgctg tcccgcgcgc cgtgccggcc ttacgacatc
     2941 gagcgcgacg gcatctcgat cggcgagtcc gcagcgttct tcctgctcga gcgcgcgcct
     3001 cgcgagggat cgatcgcgct actcggtgcc ggcgaatcga gcgacgcaca tcacatgtcg
     3061 gcccctcatc ccgagggcgc gggcgcgcgc cggtcgatgg aggtcgcgct cgcttccgcg
     3121 ggcctcgtgc cggagacgat cgactacgtg aatctgcatg gcaccggcac gccaagcaat
     3181 gatgctgcgg aggacattgc cgtgcatgca ctcttcggtg acgcggtgcc ggtcaattcg
     3241 accaagggca tgacgggcca cacgctcggc gccgccggcg ccgtcgaggc gatcgtgtcg
     3301 gtgatcgcca tcgaggaggg gcgcgttccc ggcagcgccg gcacgcggac cgtcgacccg
     3361 gcgctgcgtg cgcgctacga agtgcacggg agcgcgcgca acatcgcgcg cgtcctcagc
     3421 aattcgttcg gtttcggtgg ctcgaactgc agcctggttt ttggtcgcgt cgacgggatc
     3481 gatcgatgag ccttgcggcg cgcgtggaag gtgttggact catcgggccc ggactggcgg
     3541 gctggccgcg ggcgcgcgac gtgctctgcg ggcgcgctgc gtacgcgcca tccccgacga
     3601 gcatcccggc gcccgaagcg ctccccgccg tcgagcgccg acgggcaggc aagtgcgtgc
     3661 gcatcgcgct cgcggccgga ctcgaagcgg tggctgcggc gggccgcgag ccacgcgaca
     3721 tgggcgccgt gttcacgtcg tccaccggcg acggcgagaa cgtgcatgcg atctgcgaga
     3781 tgctcgcatc caacgatcgc ctgatctcgc cgacgcgctt ccataactcc gtgcacaacg
     3841 ctcccgccgg ctattggggt atcgcgaccg gctcgcggcg ctccgccgac agtctcgccg
     3901 cattcgatgc gagcttcggg gccggcgtcg tcgaggcaat gtctcgaatc gctgcctgcc
     3961 ccgacgaggc ggtgctgatc attgcgtacg acgcccctta tccggagccg ttgaacgcaa
     4021 cgcgtccgat tcccgatgcc ttcgcggtgg cgcttgcgct cgcatcgacg cgggcggcga
     4081 cgcgcggcgc aacgatcacg ctcgagaccg gggccggcgt ggctgatgtt ctcgacgatc
     4141 cgcagctcga gcggttgcga cgagcgatcc ccgccgcacg ctcgctgccg ctcttgcgtc
     4201 ttttggccca ggaacgaaac ggcgaggcaa cgctcgacta tctcgaaggc ctttccctga
     4261 cgttgcgccg gtcatgatgc gcgcgccggt cgaccggacg tggctgctcg cgaacctgcc
     4321 acaccaggac gggatgaatc tgctcgacgg aatcgtcgcg tgggacgcgt cgagcgtttg
     4381 cgccatcgct cgcaaccatc gcacgatcga tcatccgttg cgccgctgcg gtgagctgcc
     4441 agccgtctgc ggcatcgagt acggcgcgca ggcggccgcg gcgcacggcg cgctcgcgtc
     4501 gcgagagtca ccgggcgctg gcttgctcgc cagcgtccgc tccgtagtca tgcatgtgcg
     4561 ccgcctggat gacgtaccgg gcgacctcga tgtgttcgcc gagcgcctcg gcgggaacga
     4621 cgacggcgtc ctgtatcgat tcgaagtggc ggcagcgggc cgcctgctcg tcgagggacg
     4681 tgttgcggtg gcgttcccgc ggtgaggaag cgcgcgctcg tcaccggcgg cagcggcgcg
     4741 atcggctcgg cgatctgccg gcgtctcgcg accgacggcc tcgaggtagt cgtgcacgcc
     4801 aatggcaatc tcgaatcggc ccgccgactc gcggccgaca tcggcggcag cgcagtggcg
     4861 ttcgacgttg tcgatgcgaa ggcatccctc gcggcgctcg aactggtgct cgaggcggga
     4921 ccgatccagg tgctcgtgaa caacgctggc gtgcacgacg acgctgcctt cccgggtatg
     4981 cagcgcgaac agtggtcgcg cgtgatcgcc gttgcgatcg acggcttcta caacgtgacc
     5041 catccgctgt cactgccaat gatccgcacg cgctggggac gcatcatcag catcacgtcg
     5101 gtgaccgcac agatcggcaa tcgcggccag acgaactacg cggcggcgaa gggtgcgctg
     5161 cacgcgatca gccggtcgct cgcgcgcgag ctgggcagcc ggggcatcac cgtcaattgc
     5221 gtctcgccgg gcctgatcga caccgcgatg agcgcaggcg ccttcgatgc cggtgccatc
     5281 gcgcgactgg tgccgctgca gcgcgccggg accgcagtcg aggtcgccga cgcggtgggt
     5341 ttcctcgcgt cggacgcggc gagctacatc accggacaga tcctgccggt caacggcggg
     5401 atggcatagc cttgcgcaat agcagaatcg gcaaccgcag cagggactcg aggacgagtc
     5461 tcgtgtgcat ccacgtgagg agtgcgttgt cgcgatagta gttgaagtgc gagacgcccc
     5521 cctcctcgcg tttcaggtat cggaccggcg cgcgaacgtt gaccggtggg aggccacgcc
     5581 acaccagccg taccgcggcc tcggggtcga agtcaaagcg tcgcatccag ggcgaatcgc
     5641 gcatcaccgc ggcaagatcg gcgatcggat acacacgaaa accaaacagc gagtccccga
     5701 tacctcccgc ggtttcgagg ttggcgaacc cgttggatag cttgcgcccc atcacgcgga
     5761 ttcgcggtgc gctcgcatcg aataccggat cgccgagaat cagcgagccc cgcgccgcca
     5821 tcgagcgcgc catgaactcg ggaatgctcg ccgcgggatg ctgcgcatcg gagtccattg
     5881 tcagcgcgtg cgtgtagcca tcggccgcag cggcgcggat gccgtgcagg actgctgcgc
     5941 ccttgccctg gttctgcgga agcgtcagca cgcgcaagcc cgaatcggtt ttcgcgagtc
     6001 cgcgcagaac gtccgcggtt ccatccgtgc tgccatcgac gacgacccac accggattcc
     6061 agaactgccg cgcaccgcgc acggtctcgt acacggctgg accggggttg tagctcggaa
     6121 tgaggacgag atgggttgcg gaccgctcaa ccacgcgtga ggcgcatttc ggaatcgtcg
     6181 cggatgttga acgctcgccg ccgccagccg gcgagcgcgc ggcgcaagtg cgcgagcgag
     6241 aacaggtagt agacgagtcg gaatctgcgc agtccgggcc tgatgtcggt attgccgaaa
     6301 aggtcgcccg cgagcaccga aaggatcgcc ttcttcatcc cgccacgctc gctcgggaac
     6361 atgaacagtt cgcgcagaat gggattcgtc acgcggtaga tgaaccacga gaattccttc
     6421 ggccctcggc gcatcacgta gtcgaatcgc tcgcgcgcct tcgtctccgc cggcttgtcg
     6481 gcggaaagcc gcgcatcgac gacatccgcg cccgcgaagc cgctgttcat cgcgagcatc
     6541 acgccggagg agaacatcgg gtcgatgaac gcatacgcgt cgcctatcag gatgaagttc
     6601 tcgccctggc aggcacgcat ctcgtacgaa tagttgccgg tcgcctgcgg ctcggcgacg
     6661 agctcggcgt cggcgagtcg cgcggcgaga ggcggaagca gcgcgatcgt gtcgaggaag
     6721 tacgcgcgaa gcggcttgtc gcgcgccttc atgtagtacg gccagacgac cgcgccgacg
     6781 ctggtgatgc cgtccttcag cggaatgaac cagaaccagc cgtgctcgaa ccagctgatg
     6841 atgatgttgc cgccggcttt gccctcgtca cgcttcgcat tgcggaaatg gccgaacatc
     6901 gccgagctgt tgtggtcggg attgcgccgc ttgagctcga gccggctgcc gatgaacgta
     6961 tcgcggccgc tcgcatcgac gatgaacttc gcgcgccagc gtcgagccgt tccctgcgcg
     7021 tcggtcgccg tgacctcggg catgcgatcc gcgccgccga gatcgatctc gcgcacgcga
     7081 cagccctcgc gcgcgtcggc aaccgcgcgc gatgcgcgac ggaacaggat ctcgtcgaat
     7141 tcggagcggc gcacctggta ggccatcggc atcgacgggt cgagggcgtc ggcgaattcg
     7201 aatcccgaaa tacgatcgtg ccacggcgat acgaatgtcg caccccactt ctccatcccg
     7261 atcgcccgga tctcgtcggc cacgccgagc ttatcgaaca acggcaggtt tgcgggcagc
     7321 agcgattcgc cgatgtggaa tcgcggatgg gtttccttct cgagcagcac gacgcgatag
     7381 ccgcgatcgg cgagcaaggt cgacgccgtg gatcccgccg gtccgccgcc gatcacgacg
     7441 acgtcgcatt cctgcgcggg accagcgctt cgaagatctt cgttcacgcc aacacttctc
     7501 tgaaagtggc cgaagcattg taatgtagaa gcttgcgagc gatgcgacgc cgaccgcgtg
     7561 tcgcaaatcc gtagcctatg cagcgtccga tccgatacct tccacgttac ttcgctgacg
     7621 agcgcggccg ccgcgatttt ctcgacggac tcttcgaccg cactgcgcgc gactacgact
     7681 tcgtcgagcg tctgctcggc ttgggcagcg gaagccgcta ccggcgagac gcgctgcgtc
     7741 gcgcgggact cgcgccccgc atgcggctgc tcgatgtcgc caccggaacg ggcctcgtcg
     7801 cgcgcgaggc acttgcgctg ggcggcgcgg tgatcggcct cgatccgagt gcaggaatgc
     7861 tcgccgaagc gcggcggctt gcgtatccgc tcgtgcgcgg cctcggtgag cggctgccct
     7921 ttcgagacga atgtttcgat ttcgtcagca tgggattcgc gctgcgtcac gtcgccgaca
     7981 tcgatgcgtt gttcgcggag atgcggcgcg tgctcgtacc gggcgggacg gcctgcgttc
     8041 tcgagatcac acgccccgcg aaccggctcg tggcagggcc actgcgcgtg ttcatgacgc
     8101 gcgtcgttcc cgccgttgcg cgcgtcgcgc gccgcgacgg cgcgtccctg atgcagttct
     8161 actgggacac catcgacgcc tgcgtgccgc cggaggcggt tctcgcatcg cttgcgcgcg
     8221 ccggatttcc ttcgcccgcc agaaacgtcg cgttggggat gttctcggag tacgtcgcgc
     8281 gcaagcgatg aagtagagtg gatgccgtca ttcaccgacc gccgtcggtg aagccgggcg
     8341 ttccttttgc ctcgaagctg tctcagtcgc tgtcgtcgtc gacgcccgtc gtcgcgcgcg
     8401 tcaaccggtc gcgaatcgcc gcccacgccg tgccgtcggg cacgctctcg atcaatatcg
     8461 catcggcttt ggcgtcgtcg agctcgcgca ggttcgcata aagcaagcgc gcgtaatcag
     8521 cggcgtcgcg cggcgccata agccagacac cgtcgaacgc ttcgggtgcc tcggcggtgc
     8581 gcgcgagcac ggcaacgcat tcgtcgcgcg cctcgagttg cgcgagctcg gcgcgcagca
     8641 cgtcctgctc gaccatatga gcgggcgttc gcggcgcata gtgcgacgcg agcgttcccg
     8701 atgcgcgcgg tgcggaagaa tcgggttgcg cgagcgggca gccaagcgcc gcctcgatgg
     8761 cttcggccgc gatgccgccc ggtcgcagca gcatcggcgt atccgtcgtc agggcaatga
     8821 tcgtgctctc gatgccgatg ccggatgcgc caccgtcgag gatcaggtcg acggcatcgc
     8881 cgagatcgtc ggcgacatgg cgcgccgtcg tcggtgatac gtggccgaac cggttcgccg
     8941 atggcgctgc aatgccgccg ccgaattcct tcaacagcgc gtgcgcaacg ggatgtgacg
     9001 gcacgcgaag ccctacgctc gactgaccac cagtgacgat gtcgtcgacg tgcgcggcgc
     9061 ggggcacgat caatgtcaat ggcccaggcc agaacgcatt cgcgagcacg atcgcttgtg
     9121 ccgacagcgt cgcccagcga tgcgcggcgt cgatatcgcc gacgtgaacg atcagcggat
     9181 ggtcggaagg cctccgcttc gcgtcgaaaa tccgcgcgat cgcttttgcg ttgcccgcgt
     9241 cggcgcccaa accgtagacg gtctcggttg ggaacgcgac gaggccgccg tcgcgcagcg
     9301 tccgtgcagc acgcgtcacg gcggcgcgat cagcgcgaac gatgcgcgcg ctcacgcggg
     9361 ccgttcgccg tgggtcggca tcgcgaccga ccgtcgatcg ccttccgcgg ccccccggcg
     9421 gagcctgtcg acggaagggg gtctgttaga attcatgtct ttgcgcgacg cggcatcgac
     9481 cgtggcgtgc ccgttgcgga gcgttggatg agcgaattcc tgtttacgtc ggaatcggtg
     9541 tcggagggcc atcccgacaa ggtcgcggac cagatttcgg atgcggtgct ggacgcgatt
     9601 ctagagcacg acccgacggg tcgcgtggcg gccgagacgc tcgtgtcgac cggtctcgtc
     9661 gtcatggccg gcgagatcac gacgagcgcg aacgtcgact acggacgcgt cgcgcgcgac
     9721 acgattcgcc gcatcggtta caacgatgcc gcattgcgtt tcgatgccga cggttgtgcc
     9781 gtcatggtgt gctacggacg ccagtcgccg gacatcgcgc aaggtgtcga tcgcgcctcc
     9841 gacgattacc tgaaccaggg tgccggcgac caggggttga tgttcggtta cgcgtgcgac
     9901 gagacgccga cactgatgcc gttcccgctc tattacgcgc accggctcgt gcagcggcaa
     9961 agcgaagtgc ggcgcgacgg ccgtcttgcg tggctgcggc ccgatgcgaa atcgcaggtg
    10021 accgttcgct atgtcgacgg caagccgaag agcgtcgata cggtcgtgct gtcgacgcag
    10081 catcatccga gcatgaacga acggcagaag gaacttgccg aggcggtgat cgaggagatc
    10141 gtgcggcccg tgtttccgaa ggatatgctc tccgggacgc gcttcatggt gaacccgacg
    10201 ggccgcttcg aaatcggcgg ccctcacggc gatgcgggtg tcacgggacg caagatcatc
    10261 gtcgacacgt acggcggcgc cgcgccgcac ggcggcggcg cgttctccgg caaggacccg
    10321 tcgaaggtcg atcgctcggc cgcttacgcg gcgcgctatg tcgcgaagaa cgtcgttgcg
    10381 gccggtctcg ccaagcaatg ccaggtgcag ttgtcgtacg cgatcggtgt cgcgaaaccg
    10441 atcaacgtga ccgtgtacac cgaaggcacg ggccggatct cggatgcgcg catcgtcgag
    10501 ctcgtcaacg cgcacttcga ccttcggccg aaaggcatta tccggatgct cgacctgttg
    10561 cggccgatat accggaagac cgccgcgtac ggccacttcg gccgcgacga gcccgagttc
    10621 acgtgggaag cgaccgacaa ggtcgagatg ctgcgcgagg ccgcgggcct gcgcgaagcc
    10681 gccgttgccg ctgactgatc cggcgcacgc cgcggatcga cggcccggtc tgttcgatcc
    10741 gcttcgcctg cgggagctca agcttccgaa ccgcatcgtc gtatcgccga tgtgccagta
    10801 ctcggcgatc gatggttgtg cgacggactg gcacttcatt cattggggcc agctgctgat
    10861 gtccggcgcc ggtcttttca tgatcgaagc gaccgcggtt acccgtgacg ggcggatcac
    10921 gcccgggtgc ctcggcctct acgacgatgc atgtgaagaa gcgctgacgc gcacgctcga
    10981 acgtgcgcgc aagcaatgtc cgccaatcat tgtcggtatg cagctcgcgc acgcgggacg
    11041 caaagggtcg tcgcacgtgc cgtgggaagg cggccagctc attccactgg acgccggggg
    11101 ctggatcccc gtggcgcctt cggcattgcc gcacgcgccg accgagcggc cgcctgccgc
    11161 actcgacgcc gcgggcctcg ccgcgatacg cgacgcgttc gtggacagcg cgcgtcgcgc
    11221 cgctcgcgcg ggtatcgatg cgctcgaact gcacatggcg cacggctatc tgctgcacga
    11281 attcctgtcg ccgctcgcaa acgtccgcac cgacgcgtat ggaggatcgt tcgagcgcag
    11341 gatcgccttc ccgctcgaag tgttcgacgc cgttcgcgcg gcgtggccgg ctgcgcggcc
    11401 gcttggcgtg cggctgtcgt gcacggactg ggtcgacggc ggatggacgc tcgaggaatc
    11461 gaccgaattc gcgcgacgtc tcgccgcgcg cggctctgac tggatcgatg cgtcgagcgg
    11521 cggcgtttcg ccggcgcaga agattccgct cggtccaggc tatcaggttc atctatcgcg
    11581 cgaaattcgt cgtgcgactg ctggcgcgcg aacgatcgcg gtgggcctca tcaccacacc
    11641 cgcacaggcc caggcgatca tcgcgtccgg tgatgcggat ctcgtcgcga tggcgcgcgc
    11701 attcctgtgg gatccgcgct gggtgtggca cgcggcggcg gaacttggcg ccgcggttgc
    11761 cccgccgcca caatacctgc gcagcgcgcc gcgcgatgcg gcaagcgtgt tcaccggtgc
    11821 gaggatcggt cagcggtaat caatccagac ggcggggacc cgtacatcgc ggcacggtat
    11881 aatcgccaat ttccgcgcgc tttcgcggac cttccaagaa tacggagcta caaagatggc
    11941 cgaacgcacc cgcacccgcc gcaacacgct cgagcgccgc tgcctgccga agacattcaa
    12001 gcggatgttc gtcggcgcgc cgaagggtct tttcaagatg ctggagaaga tcaagaaggc
    12061 ctgacgccat gggccccgac gggcccggtc gacttgtccg tagggccgcg tcgcggccct
    12121 ttttgcatca tcaaggcgtg tgcgcgtcgc cctgttggat ccgcgttttg cgcatcgacg
    12181 ccgccagcag ccagcatcgc tttgtgcaag cgttgctgcg tccgcacgat gatgccgcgc
    12241 agcgatccca tgcccttcgc ttactacaac cggctgacgg ctgaacaaaa gcggatctat
    12301 cgcgcaagcg atgcgattga atcgatcgca ctgccgccgc tcgccgcggt cggcagcgac
    12361 gttggcgcgg tccgtacggc gcttctgaac gacgacgcgc gcgccgcgca gcgtgcctgc
    12421 cagcggatcg ccgacgcgtt gacgacggtt tgccgtgtac cgcccttgag gattcgcgtg
    12481 ctggcgcagc ggccttccga cgactatggc gagctgcatg ggttgtacga gcctgtcgaa
    12541 ggcaggcgcc gcgcgacgat cagcgtctgg atgcgaacgg cgaagaaacg acaggttgtc
    12601 gcattcaaga gcttcctgcg cacactgtgc cacgagcttt gtcaccattt cgattacgag
    12661 ttcttcgcgt tcgaggaaac gtttcacacc gacggcttct acaagcggga gtcgagcctc
    12721 gcgaatgcgc tgctcgtgag cgaggcgcgc acgacgactc catcgatatt gggttcgtcc
    12781 gtcggcgatc tctgaatcgg catcgcgcgc gcatttgcta caatgcgcgc tgacgccccg
    12841 aggagcgttg caacccggca cgacgccggg ccaggctcgg ggccgggaca ttcgcctgga
    12901 tgttccgttg caacggcgct cgttgaaccg cgacaaacgg cggccaacgg gtggtcattc
    12961 ccctcgcagc ccccgatcgt cgcccgtcat caacggagtg actgatgaat gcagtgaacg
    13021 aaacggggca attcgcagac tacgaagtcg ccgatctggc gctcgccgat tggggtcgca
    13081 aggaaatcgc gatcgccgag acggaaatgc cgggattgat gtcgatacgc gacgagttcg
    13141 cgccatcgca gccgctgcgc ggcgcacgga tcgccggcag cctgcacatg acgatccaga
    13201 ccgccgtgtt gatcgagacc ctgcaagcgc tcggtgccga cgtccgctgg gcgtcgtgca
    13261 acatctattc gacgcaggat cacgccgcgg ccgcaatcgc cgcgcgcggc acgccggtgt
    13321 tcgcgtacaa gggcgaaacg ctcgagcggt actgggacct cacccaccgc atcttcgaat
    13381 ggccgggtgg actcgcgccg aacatgattc tcgacgacgg cggcgatgca acgctgctcg
    13441 tacatcttgg tgcacaagcg gaaaaggatc gctcggtcat cgctcatcca acgagcgacg
    13501 aggagaaagc gctgtttgcg tcgatcgcgg tgcggctcga caagacgccg gatttctatt
    13561 cgacgatcaa gcgcggcatt cgcggcgtca ccgaggagac gacgacgggc gtcaaacggc
    13621 tctaccagat gcaccgcgaa ggacggctgg gattcccggc gatcaacgtc aacgattcgg
    13681 tgacgaagag caagttcgac aacctctacg gctgccgcga gtcgctcgtc gatggcatca
    13741 agcgcgcgac ggacgtgatg gtcgcgggca agatcgcgct cgtctgcggc tacggcgatg
    13801 tcggcaaggg atctgcgcag gcgctgcgcg cgctttcagc gcaggtgtgg atcgccgaaa
    13861 tcgatccgat ctgcgcgctg caggcggcga tggaaggcta ccgtgtcgta accgtcgacg
    13921 atgcgtgtga caaggtcgac atcgtcgtca ccgcgacagg caacatcgac gtcatcacct
    13981 acgcgcacat ggcgcggatg aagaacaaca cgatcgtctg caacatcggc cacttcgaca
    14041 acgagatcga cgtcgagtcg ctgaaggctt acaagtggga gaacatcaag ccgcaggtcg
    14101 atcacgtgat cttcgacgac ggcaagcgga tcatcctgct ggcggaaggg aggct
//
LOCUS       DASSVX010000290         4814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_129873, whole genome shotgun sequence.
ACCESSION   DASSVX010000290 DASSVX010000000
VERSION     DASSVX010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129873"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VFO53_12570"
     CDS             <1..915
                     /locus_tag="VFO53_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET8877156.1"
                     /translation="GKEIDLRVSTVPTMHGESVVMRILDKGGVALDFAKLGFEDDVLE
                     VFLDVLLQPHGVLLVTGPTGSGKTTTLYTALDRLNQPDVKILTVEDPVEYQMPGINQI
                     QVKPQIDLTFANALRSIVRQDPDVIMIGEIRDLETAQIAVQSALTGHLVLSTVHTNDA
                     PSTVNRLLDMGVEDYLLTSTVIGILAQRLVRKLCEHCKEPYVALPELVEQMKLAKFSG
                     DARITLWHAKGCSHCAQTGYTGRICITEIMPMTDGLRSLVMKHANAAELRTEAIRAGM
                     VSMYEDGLRKAVKGVTTFEEVLRATRED"
     gene            936..2171
                     /locus_tag="VFO53_12575"
     CDS             936..2171
                     /locus_tag="VFO53_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET8877157.1"
                     /translation="MPRYSYRAVTAAGDVSSGELEAANESEIVDRLRDQGLMPMQIAQ
                     SLGVAAAGAAVARPPRQSLFAPKRVTRDNVLGITRELATLLRAGLTLDRALELLISLA
                     TSLPVAVMLQGIRDEVRGGKALSQALDARRDVFSRFYVNIVRAGEAGGALGTVLARLA
                     ETMERNKELRDSVRSALIYPTILIFVAVASIMILLIYVVPQFQSTFAQAGKALPVPTQ
                     VVIAVGTFLRNWWWALIPGAALAVLWFRRRGRVPAIRRVRDARLLRMPLLGDLIAKVE
                     IARFARTLSTLLANGVTLLAGLAIVKETMGNVVLSTGFDGVIAKLREGKGFGRPMADT
                     GLYPRLATQMILVGEESGRLEEMLSRVADVYDREVATAVKRFLAVLEPVLILGLAVMV
                     GGIVFSILLGVMGMSELVV"
     gene            2283..2723
                     /gene="gspG"
                     /locus_tag="VFO53_12580"
     CDS             2283..2723
                     /gene="gspG"
                     /locus_tag="VFO53_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HET8877158.1"
                     /translation="MRSRSHASRSPRQRGMTLIEILVVLTLIGVVLGIVGSNYLGKSE
                     QAKAKAAKIEIDQIGQTLDLFKLELGRYPTTQEGLQALVVAPPGLSNWAGPYWKKSTA
                     PKDPWGHDYKYASPGAHGAYDIISLGADGVEGGEGANKDIVNWE"
     gene            2773..3204
                     /locus_tag="VFO53_12585"
     CDS             2773..3204
                     /locus_tag="VFO53_12585"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HET8877159.1"
                     /translation="MKGVTLLELLIVLALMAIVAGFVMPMFGGPVSTSELRATARQLA
                     AGLRLAQSEAVSQRRQTFLVLDVAGKRFKVDNDPHEHKLSSKIELKLFTAQNDLVSES
                     VGSIRFFPDGGSNGGRITVAAGERKFDVDIDWLTGRVAIVD"
     gene            3249..3674
                     /locus_tag="VFO53_12590"
     CDS             3249..3674
                     /locus_tag="VFO53_12590"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET8877160.1"
                     /translation="MKPTHAHRGFSVLEVLVAFIIVALVVTALFQLFGGALRNASGAG
                     DWTRALLVAQSRLELAASTQPLREASDAGTEPDGRIAWRASVTPYVPPEPNPDLERAS
                     ESMPTRLFRINVDVTFSGDDGKPRTVSLSTLRIGQRNPA"
     gene            3757..4539
                     /locus_tag="VFO53_12595"
     CDS             3757..4539
                     /locus_tag="VFO53_12595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET8877161.1"
                     /translation="MRAAGPPQGSNCAPVGGSEAAKPQACGNHTSEAGIAQRGIAQRG
                     FTLLELIIALVLLGLLSAVLFGSVKLAGRSTDSGEARAGAASSMRLAQQFLRTNLEAQ
                     HPLRMRKMADWPLLFAGASDELRYAARLPARVAGGGIWYYRLVVKSDAARSPLVLERM
                     IPDLQADALPDFVNADRSVLAEDIASLKIGYFGREADAAPTVAPSWRDQWSDTQRLPL
                     MIRIDVTPKTGAPWPTLYVSPREAPEAGCRAWDIAREHCAAV"
     gene            4632..>4814
                     /locus_tag="VFO53_12600"
     CDS             4632..>4814
                     /locus_tag="VFO53_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HET8877162.1"
                     /translation="MPHVVNRSMQSGIALVLALWITVLLTVLASGFAFSLRSEALATR
                     NAVSLARVRALADGALE"
BASE COUNT          770 a         1603 c         1658 g          783 t
ORIGIN      
        1 ggcaaggaga tcgatctgcg cgtgtcgacc gtgcccacga tgcacggcga atcggtcgtg
       61 atgcgcatcc tcgacaaggg cggcgtcgcg ctcgatttcg cgaagctcgg cttcgaggac
      121 gacgtgctcg aggtgtttct cgacgtgctg ctgcagccgc acggcgtgct gctcgtgacg
      181 ggtccgaccg gctcgggcaa gacgacgacg ctctataccg cactcgaccg gctgaaccag
      241 cccgacgtca agatcctgac ggtcgaggac ccggtcgagt accagatgcc gggaatcaac
      301 cagattcagg tcaagccgca gatcgacctc acgttcgcca acgcgctgcg ctcgatcgtg
      361 cgccaggatc ccgacgtgat catgatcggc gagatccgcg acctcgagac ggcgcagatc
      421 gccgtgcaat cggcactgac cggtcacctc gtgctgtcca ccgtgcacac gaacgacgcg
      481 ccgtcgacgg tcaaccggct gctcgacatg ggcgttgagg attacctgct cacgtcgacg
      541 gtgatcggca tcctcgcgca gcggctggtg cgcaagctgt gcgagcattg caaggagccg
      601 tatgtcgcgc tgcccgagct cgtcgagcag atgaagctcg cgaaattctc cggcgacgcg
      661 aggatcacgc tgtggcatgc gaagggctgc agccactgcg cgcaaaccgg ctataccggc
      721 cgcatctgca tcaccgagat catgccgatg accgacgggc tgcgcagcct cgtgatgaag
      781 catgcgaacg ccgccgagct tcgcaccgaa gcgatccgcg ccggcatggt gtcgatgtac
      841 gaagacggcc tgcgcaaggc cgtgaagggc gtgacgacat tcgaggaagt cctgcgcgcg
      901 acgcgcgagg actgacgtcg cgggagcatt tgccgatgcc gcgctacagc tatcgcgccg
      961 tcaccgctgc cggcgacgtg tcgagcggcg agctcgaggc agcgaacgaa tccgagatcg
     1021 tcgaccgcct gcgcgaccag ggcctgatgc cgatgcagat cgcgcagtcg ctcggtgtcg
     1081 ccgccgcggg tgccgccgtg gcgcgacccc cgcgccagag cctcttcgcg ccgaagcgcg
     1141 tcacgcgcga caacgtgctc ggcatcacgc gcgagctcgc gacgctgctt cgcgcgggac
     1201 tcacgctcga ccgcgcgctc gagcttttga tcagcctcgc cacgtcgctt cccgtcgcgg
     1261 tgatgctgca gggcatccgc gacgaagtgc gcggcggcaa ggcgctgtcg caggcgctcg
     1321 acgcgcggcg cgacgttttc tcgcgcttct atgtcaacat cgttcgcgcc ggggaggcgg
     1381 gcggtgcgct cggcaccgtt ctcgcgcggc tcgccgagac gatggagcgc aacaaggagc
     1441 tgcgggattc ggtgcgctcg gcgctgatct atccgacgat cctgatcttc gtcgcggtcg
     1501 cgtcgatcat gatcctgctg atctatgtcg tcccgcaatt ccagtcgacg ttcgcgcagg
     1561 cgggcaaggc gctgcccgtt cccacgcagg tggtcatcgc cgtcggcacg tttctgcgca
     1621 actggtggtg ggcgctgatc cccggcgcgg cacttgccgt cctgtggttt cgccgccgcg
     1681 gccgcgtgcc ggcgatacgg cgcgtccgcg atgcgcggct tttgcgcatg ccgctgctcg
     1741 gcgacctgat cgcgaaagtg gaaatcgcgc gcttcgcgcg cacgctgtcg acgctgctcg
     1801 ccaacggcgt gacgctgctc gcggggcttg cgatcgtcaa ggaaacgatg ggcaacgtcg
     1861 tgctgtccac cggcttcgac ggcgtgatcg cgaagctgcg cgaggggaag ggattcggtc
     1921 gcccgatggc cgacacgggc ttgtatccgc ggcttgcaac gcagatgatc ctcgtcggcg
     1981 aggagagcgg gcggctcgag gaaatgctgt cgcgcgtggc cgatgtctac gatcgcgaag
     2041 tggccacggc ggtcaagcgc tttctcgccg tgctcgagcc cgtgctgatc ctcgggctcg
     2101 ccgtcatggt gggcggcatc gtgttctcga tcctgctcgg cgtgatgggc atgagcgaac
     2161 tggtcgtgtg attcctgtct aaggaacgcc tgttcggcgc ttccacagga tttttccggg
     2221 cggcggcaca ttcgacgccg ccactcgcaa ggacattcga gatgatgaac agcaatcgtt
     2281 ggatgcgatc ccggtcgcat gcgtcgcgca gtccgcggca gcgcggcatg acgctgatcg
     2341 agatactcgt cgtgctgacg ctgatcggcg tcgtgctcgg catcgtgggc agcaactacc
     2401 tcggcaagag cgagcaggca aaggccaagg cggcgaagat cgagatcgac cagatcggcc
     2461 agacgctcga cctgttcaag ctcgagctcg gccgctatcc aaccacgcag gaaggcttgc
     2521 aggctttggt ggtcgcgccg cccgggcttt cgaactgggc cggcccgtac tggaagaagt
     2581 ccaccgcacc gaaggatccg tgggggcacg actacaaata cgcgtccccc ggcgcgcatg
     2641 gcgcctacga catcatctcg ctcggcgccg atggcgtgga aggcggtgaa ggagcgaaca
     2701 aggacatcgt gaattgggaa tgagcgcggc gccgcgcgag ttccgcgagg ccgacggccg
     2761 agccgaggtg acatgaaggg cgtcacactc ctcgagctgc tgatcgtgct cgcgctgatg
     2821 gcgatcgtcg cgggcttcgt gatgccgatg ttcggcggtc cggtgtcgac gagcgagttg
     2881 cgcgcgaccg cgcgacagct cgcggccggc ttgcggcttg cacaaagcga agccgtgtcg
     2941 cagcggcgcc agacgttcct cgtgctcgac gtcgcgggca agcggttcaa ggtcgacaac
     3001 gatccgcacg agcacaagct gtcgtcgaag atcgagctca agctcttcac cgcgcagaac
     3061 gacctcgtga gcgagagcgt ggggtcgatc cgcttctttc ccgatggcgg cagcaacggc
     3121 gggcgcatca ccgtcgccgc cggcgagcgc aaattcgacg tcgacatcga ctggctcacc
     3181 ggccgcgtcg cgatcgtcga ttgacgatga acccggatgc gtcgccgcca cgccggtgtc
     3241 gatcgaccgt gaagcccacg cacgcgcatc gcggcttctc ggtcctcgag gtgctggttg
     3301 cgttcatcat cgtggcgctc gtcgtcaccg cgctcttcca gctctttggc ggggcgctcc
     3361 gcaacgcgag cggcgccggc gactggacgc gcgcgctgct ggtggcgcag agccggctcg
     3421 agctcgccgc ttcgacgcag ccgctgcgcg aggcgagcga cgcgggcacc gagcccgacg
     3481 ggcgcattgc gtggcgcgcg tcggtgacgc cttacgttcc ccccgagccg aaccccgatc
     3541 tcgagcgcgc gagcgagtcg atgccgaccc ggctcttccg tatcaacgtc gacgtcacct
     3601 tttccggcga cgacggcaag ccgcgcaccg tttcgctgtc gacgctgcgc atcggccagc
     3661 gcaacccggc atgagagccg ccgggccgcc ccaaggctcg aattgcgccc ccgtgggggg
     3721 cagcgaagcg gcgaagccgc gagcgtgggg ggatcaatga gagccgccgg gccgccgcag
     3781 ggctcgaatt gcgcccccgt ggggggcagc gaagcggcga agccgcaagc gtgcgggaat
     3841 cacacaagcg aggcgggcat cgcgcaacgc ggcatcgcgc aacgcggctt cacgctgctc
     3901 gagctcatca tcgcgctcgt gctgctcgga ttgctgtccg ccgtgctgtt cggttcggtg
     3961 aagctcgccg ggcgcagcac cgacagcggc gaggcgcggg cgggtgcggc gtcgtcgatg
     4021 cgtcttgcgc aacagttcct gcgcaccaat ctcgaggcgc agcatccgct gcgcatgcgc
     4081 aagatggccg actggccgtt gctcttcgcg ggcgcgagcg acgagctgcg ctacgcagcg
     4141 aggctgccgg cgcgcgtcgc cggcggtggc atctggtact accggctggt ggtgaagtcc
     4201 gacgccgcgc gctcgccgct cgtgctcgaa cgcatgattc ccgatctgca ggccgacgcg
     4261 ctccccgatt tcgtcaatgc cgaccgctcg gtgcttgccg aagacatcgc ctcgctcaag
     4321 atcggctatt tcggccgcga ggccgatgcc gcgccgaccg tcgcaccgtc gtggcgcgac
     4381 cagtggagcg atacgcagcg cctgccgctg atgattcgca tcgacgtcac gccgaagacc
     4441 ggtgcaccgt ggccgacact ctatgtctcc ccgcgcgagg cacccgaggc gggctgccgc
     4501 gcatgggaca tcgcgcgcga gcattgcgcc gccgtatgag ggccgcagag ccgccgcaag
     4561 gcgcgaatag cgccccttca gggagcagcg cagcggcgaa gccgcaagcg tgggggggtc
     4621 ataccaagcc gatgccgcac gtcgtcaacc gcagcatgca atccgggatt gcgctggtgc
     4681 tcgcgctgtg gatcaccgtc ttgttgacgg tcctcgcgag cggattcgcg ttcagcttgc
     4741 gcagcgaggc gctggcaacg cgcaacgcgg tctcgctcgc tcgcgtccgc gcgctcgccg
     4801 acggcgccct cgag
//
LOCUS       DASSVX010000291         3541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_712930, whole genome shotgun sequence.
ACCESSION   DASSVX010000291 DASSVX010000000
VERSION     DASSVX010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712930"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..881)
                     /locus_tag="VFO53_12605"
     CDS             complement(171..881)
                     /locus_tag="VFO53_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8877163.1"
                     /translation="MSALVEARDVHAHYGESHVLRGVSLSINAGESLGLLGRNGMGKT
                     TLIRTLIGLTSASAGNVLIHGRDCTREPAHRIARMGVAYVPEGRGIFPNLSVRENLLF
                     PARPSRDARTGWTYERVLDTFPRLRARIASGGHQLSGGEQQMLAIGRALMTNPELLIL
                     DEATEGLAPQIVHDIWRIVGEIRQSGIASLIVDRNYRLVLTHTDRALVLEKGVAVLDD
                     WSASLALRSDALARHLGV"
     gene            complement(932..1711)
                     /locus_tag="VFO53_12610"
     CDS             complement(932..1711)
                     /locus_tag="VFO53_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8877164.1"
                     /translation="MPADVLLDAADVSRRFGGLVAVDRASLALVRGEVHALIGTNGAG
                     KSTLVNVLSGELPATEGRVRLLGRDVTHWPQPRRARAGLGRSYQRTTIFAQATVFENC
                     RLAAQAHFQHPLDLWNMATRCPDSTELAEQAIERAALTAQAHRSAGTLSHGQKRQLEI
                     AICLATAPQVLLLDEPLAGMGPEETERMLVLLAALKPGHAILLVEHDMDAVFRIAERI
                     TVMVDGRVIASAEPDAIRGNALVQAAYLGEMVDTPESATIA"
     gene            complement(1701..2690)
                     /locus_tag="VFO53_12615"
     CDS             complement(1701..2690)
                     /locus_tag="VFO53_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8877165.1"
                     /translation="MHAGGRRVWPLAAALIALAAFPHVGGSFYIDLALTTMILSIFAL
                     SLELLVGRTGLVSFGHAAFFGIAAYATALLSPAASAASLAWLVPAVLLVTALAAAAIG
                     ALCLRTRGVYFIMVTLAFAQMTYFVFHDMKFAGGSDGRYLYVKPVLALGGVTLVDLGR
                     PRTLYYAALGCLIVVYALLALLGRSRFGHALAGIRANEQRMRAVGFSTYPYKLAAFTL
                     AGVLAGIAGFLHAVKDGFVTPEILAWHQSGAVLLMLILGGIGTLRGAVLGAFVFVLMK
                     EFFQSEALFGAFARHWQLPLGLAIIACVALMPNGVVALPTQLRRVRAPLPHAG"
     gene            complement(2719..>3541)
                     /locus_tag="VFO53_12620"
     CDS             complement(2719..>3541)
                     /locus_tag="VFO53_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8877166.1"
                     /translation="FLVAIGLTLIFGILGVINLAHGSFYMIGAYLAYALAPWAATHLG
                     AGFFGALAIGLVLSIVLGYVLEWAFVSYLYEREHLQQVLMTYGLILVFEELRSIIAGD
                     DVHGVAPPAWLSGSVPLGDVMTYPVYRLFMSGVCIAIALALYAVLTRTRVGMAIRAGA
                     SNREMVQSLGVDIRLLYRIVFAAGVALAVFAGMIAAPVSSVYPGMGNQVLIVSFVVVV
                     IGGIGSVRGALLASLLVGFVDTFGKVFIPQIAAILVYVLMALILLWRPEGLFRTG"
BASE COUNT          666 a         1181 c         1225 g          469 t
ORIGIN      
        1 gatgcgatgc ggtgcttcgt agaagacgag cgccagcggc catgtcgacg ccgtcgcgag
       61 ccattcgcgg cgcgccttcg cctgcgcagg caggaacccg atgaacgcga atcgctcggc
      121 aacgaggccg gccgccgtca gcgtccgaca aacagcggat cagccagcat ctacacgccg
      181 agatggcgcg cgagcgcgtc gctgcgcagc gcaagcgatg cggaccagtc gtcgagcacg
      241 gcgaccccct tttcgagcac cagcgcgcga tcggtatgcg tgagcacgag gcgataattg
      301 cggtcgacga tcagcgaggc gatgccgctc tggcgtattt cgccgacgat gcgccagata
      361 tcgtgcacga tctgcggcgc gagtccctcc gtcgcttcgt cgaggatcag gagctcgggg
      421 ttcgtcatca gcgcgcggcc gatcgcgagc atctgctgct cgccccccga aagctggtga
      481 ccgccgcttg caatgcgcgc gcgcaacctc gggaacgtgt ccagcacgcg ctcgtacgtc
      541 cagccggtgc gcgcatcgcg cgacggacgc gccgggaaaa ggaggttctc gcgcaccgac
      601 aggttcggga agatgccgcg gccctcggga acatacgcga cgcccatccg ggcgatccgg
      661 tgcgccggct cgcgcgtgca gtcgcggccg tggatgagca cgttgcccgc gcttgccgat
      721 gtgaggccga tcagcgtgcg gatgagcgtc gtcttgccca tgccgttgcg gccgagcagg
      781 ccgagcgact cgccggcgtt gatcgaaagc gacacgccgc gcagcacatg gctttcaccg
      841 tagtgcgcgt gcacgtcgcg cgcttcgaca agcgcgctga tggccatcgc cggcgcgcgg
      901 atcacggtgg tgcgtgtcgc gggtgcccgc atcaggcgat cgtcgccgat tcgggcgtgt
      961 cgaccatttc accaaggtac gccgcctgca ccagcgcgtt gccgcggatc gcgtccggct
     1021 cggcactcgc aatgacgcgg ccgtcgacca tcacggtgat ccgctcggcg atgcgaaaca
     1081 cggcgtccat gtcgtgctcg acgagcagga tcgcatggcc gggcttcagc gcggcgagaa
     1141 gcaccagcat gcgctccgtc tcctcgggac ccatgcccgc gagcggctcg tcgagcagca
     1201 gcacctgtgg cgccgtcgcg aggcagatcg caatctcgag ctggcgcttc tggccatgcg
     1261 aaagcgtgcc ggcgctgcga tgcgcctgcg cggtgagtgc cgcgcgctcg atcgcctgtt
     1321 cggcgagctc ggtgctgtcg ggacagcgcg tggccatgtt ccaaaggtcg agcgggtgct
     1381 ggaagtgcgc ctgcgccgcc agccggcaat tctcgaacac cgttgcctgc gcaaagatcg
     1441 tcgtgcgctg gtagctgcgg ccgagcccgg cgcgtgcgcg tcggggttgc ggccagtgcg
     1501 tgacgtcacg tccgagaagg cggacacgac cttccgtcgc cgggagctcg cccgacagca
     1561 cgttgaccag cgtcgacttt ccggcgccgt tcgtgccgat cagcgcgtga acttcaccac
     1621 ggacgagcgc aagcgacgcg cggtcgacgg cgacgagccc gccaaagcgg cgcgacacgt
     1681 cggccgcgtc gagcaggacg tcagccggca tgcgggagcg gcgcgcgaac gcggcgcagc
     1741 tgcgtcggca acgcgacgac gccattcggc atcagcgcga cgcatgcgat gattgcgagg
     1801 ccgagcggca gctgccaatg cctggcgaag gcgccgaaaa gtgcttccga ctggaagaat
     1861 tccttcatca gcacgaacac gaaggcgccg agcaccgcgc cgcgcagcgt gccgatgccg
     1921 ccgaggatca gcatcagcag caccgcgccg gactggtgcc acgcgaggat ttccggggtg
     1981 acgaatccgt ccttcaccgc gtgcaggaag ccggcgatcc cggccagcac acccgcgagc
     2041 gtgaacgccg ccagcttgta gggataggtc gagaaaccga ctgcgcgcat ccgctgctcg
     2101 ttcgcacgga tgcccgcgag cgcatgcccg aaacgcgagc gaccgagcag cgcgagcagc
     2161 gcgtagacga cgatgaggca tccgagtgcc gcgtagtaga gcgtgcgcgg acgaccgaga
     2221 tcgaccagcg tgacgccgcc gagcgcaagc accggcttca cgtacaggta gcggccgtcg
     2281 ctgccgccgg cgaacttcat gtcgtgaaag acgaagtacg tcatctgcgc gaaggcgagg
     2341 gtgaccatga tgaaatagac gccgcgagtg cgcaggcaca gcgcgccgat cgcggccgcg
     2401 gcgagtgccg tgacgagcag taccgccgga acgagccagg cgagcgacgc cgccgaggcg
     2461 gccggcgaca gcagcgcggt tgcgtaggcc gcgatgccga aaaaagccgc gtggccgaac
     2521 gagacgagcc ccgtgcgccc gacgagaagc tcgagcgaca gcgcgaagat cgacaggatc
     2581 atcgtcgtca gcgcgagatc gatgtagaag ctgccgccca cgtgcggaaa cgccgcgagc
     2641 gcgatcagcg ctgcagcaag cggccagaca cgtctgccgc cggcgtgcat gcgtcctccg
     2701 cggcgaggcg cgctcacgtc aacccgtccg aaacaggccc tcgggccgcc atagcaggat
     2761 gagcgccatc agcacgtaga cgagaatggc ggcgatctgc gggatgaaca ccttcccgaa
     2821 ggtgtcgacg aagccgacga gcagcgacgc gaggagggcg ccgcgaaccg acccgatgcc
     2881 gccgatcacg accacgacga acgacacgat cagcacctgg ttgcccatgc cgggatagac
     2941 cgacgagacc ggcgccgcga tcatgcccgc gaacacggcg agtgcgacgc ctgccgcgaa
     3001 cacgatgcgg tagagaagac ggatgtcgac gccgagcgat tgcaccattt cgcggttgct
     3061 ggcacccgcg cggatggcca tgccgacacg cgtgcgggtg agcaccgcat aaagcgccag
     3121 cgcgatggca atgcacacgc ccgacatgaa cagacgatag accggatagg tcatcacgtc
     3181 gccgagcggc acgctgcccg aaagccacgc gggcggcgcg acgccgtgca cgtcgtcgcc
     3241 ggcgatgatg ctgcgcagct cctcgaacac gaggatcagt ccgtacgtca tcagcacctg
     3301 ctgcagatgc tcgcgctcgt acaggtagct cacgaacgcc cattcgagga cgtaaccgag
     3361 cacgatcgac agcacgagcc cgattgcaag tgcgccgaag aagcccgcgc caaggtgtgt
     3421 cgcggcccac ggcgcgagcg cgtaggcgag gtacgcgccg atcatgtaga agctgccgtg
     3481 cgcgagattg atgacgccga gtattccgaa gatcagcgtg agcccgattg cgacgaggaa
     3541 c
//
LOCUS       DASSVX010000292         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_379788, whole genome shotgun sequence.
ACCESSION   DASSVX010000292 DASSVX010000000
VERSION     DASSVX010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379788"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VFO53_12625"
     CDS             complement(<1..1134)
                     /locus_tag="VFO53_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877167.1"
                     /translation="MAWWGALAVAAGFVVAAPALAADPIKIGLVAALSGPSAQSGEAI
                     TQGLSIAIDEINAAGGVLGRPLALVKRDDESSPPKGATAARELVYDEKVAAIFGGLDT
                     PVSLAIVPIANKEHVPYMGVWAAGTPITRNGANPNYAFRVSAVDVMVDEALLKYAQKN
                     GARKPGLILINNPWGESNEKGLKEEAQKLGITLAGIEKFEDRDVDVVPQLTRLKNAGA
                     DALILVANAAPGAQMMKARERMGWKVPVISHWGVSGGRFPELAGPTAGDVVFVQTYSF
                     FGDQKPAGKKVLSELAKKYNVKGPEGVISPVGLADAYDAMHLLALAIGKAGSTKGDAV
                     REALHRIDEYPGLIKTYRKPFSAQNQDALGPDDYIFVRFDGTRI"
     gene            complement(1336..2349)
                     /locus_tag="VFO53_12630"
     CDS             complement(1336..2349)
                     /locus_tag="VFO53_12630"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET8877168.1"
                     /translation="MQIFRDLRRRAHALVAWLAARLRRAELPGRFETGSASSWRGYIT
                     TAPGVEPARDYLVYVPRGWNRRVRAPLRVLCHGCNQTPEDFAALTRIASHADRDGTLV
                     LLPRQSSSANAWGCWNWFEANTERGAGEAAIVAAQIVQVRRRYRARRERVFVAGLSAG
                     GALAAVLGLRHPRLVRGILVHSGLACGAASSPASALSVMRRGPDNDVARVADIARSAV
                     RNGAAPHVALLVVQGDRDDVVAPANATALVDQFLHFNGYAASGPAGSLPPSDASSHEA
                     GGTHYPVRTADWTRDGRVDVRLVRIEGLGHAWSGGDGGYAYADPHGTDALDLFARFIA
                     DAG"
     gene            complement(2410..>3147)
                     /locus_tag="VFO53_12635"
     CDS             complement(2410..>3147)
                     /locus_tag="VFO53_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HET8877169.1"
                     /translation="FARTFMPDLAERIEHYPGERPIFDLYGVEDEIQRALKKEVPLKS
                     GGYLVIDQTEAMTTIDVNTGGFVGGRSFDDTIFKTNLEAAQVIARQLRMRNLGGIIII
                     DFIDMENVDHRNAVLAELDKALEKDRTRLTVNGFTQLGLVEMTRKRTRESLAHVLCET
                     CPTCGGRGEFKTAQTICYEILREILREAKQFNAREFRILASQSVIDMFLDEESQSLAQ
                     LGDFIGKPISLQVETLYTQEQYDVVLI"
BASE COUNT          482 a         1069 c         1070 g          526 t
ORIGIN      
        1 gatgcgcgtg ccgtcgaagc gcacgaagat gtagtcgtcg ggtccgagcg cgtcctggtt
       61 ctgcgcgctg aacggcttgc ggtaggtctt gatcagcccg gggtactcgt cgatccggtg
      121 cagtgcctcg cgcaccgcgt cgcccttggt gctgcccgcc ttaccgatcg cgagtgccag
      181 aaggtgcatc gcgtcgtacg catcggcgag gccaaccggc gagatcacgc cttcggggcc
      241 cttgacgttg tacttcttcg cgagctcgga cagcaccttc ttgcccgcgg gcttctggtc
      301 gccgaagaag ctgtacgtct gcacgaaaac gacgtcgccc gcggtggggc cggcgagctc
      361 ggggaagcgg ccgcccgaaa cgccccagtg cgagatcacc ggcaccttcc agcccatccg
      421 ctcgcgcgcc ttcatcatct gcgcgcccgg cgcggcgttg gcgacgagga tcagcgcgtc
      481 ggccccggca ttcttgagac gcgtcaactg cggcacgacg tcgacatcgc ggtcctcgaa
      541 cttctcgatc ccggcgagcg tgatgccgag cttctgcgct tcttccttca gtcccttttc
      601 gttcgactcg ccccacgggt tgttgatgag gatgaggccc ggcttcctgg caccgttctt
      661 ctgcgcgtac ttgagcagcg cttcgtcgac catcacgtcg acggccgaga cgcggaacgc
      721 gtagttggga ttggcgccat tgcgcgtgat cggcgtgccc gccgcccaga cgcccatgta
      781 cggcacgtgc tccttgttgg cgatcggcac gatggcgagc gacaccggcg tgtcgaggcc
      841 gccgaaaatc gccgcgacct tctcgtcgta gacgagctcc cgcgccgccg tggcgccctt
      901 gggcgggctc gactcgtcgt cgcgcttgac cagcgccagc ggccggccga gcacgccgcc
      961 cgcggcgttg atctcgtcga tggcgatcga gagaccttgc gtgatcgcct cgcccgactg
     1021 cgccgagggt ccggacagcg ccgcgacgag gccgatcttg atcgggtcgg cggccagcgc
     1081 gggtgcggcg acgacgaatc cggcggcgac ggcgagtgcg ccccaccatg ccatgcgagc
     1141 gttgcgtgca attcccatag ctcctccagt cgggtcgagt aagaagcgca ttacttgccg
     1201 gcgcgccggg cgggcccgcg cgacgcgaca cgcccgcaat gctgccccaa gtccggcgcg
     1261 gtgtcacgct gcaccgcgtg cccgcagcgg gtaagcagtc tcttactacg gcatcgtgcg
     1321 acagcggggg ttatgtcaac ccgcgtcggc gatgaagcgc gcgaagaggt cgagcgcatc
     1381 ggttccgtgc ggatcggcgt aggcgtatcc gccatcgccg ccgctccatg cgtggccgag
     1441 cccctcgatt cgcacgaggc gcacatcgac gcgcccgtcg cgcgtccagt cggcggtacg
     1501 caccggatag tgcgtgccgc ccgcctcgtg cgacgacgca tcgcttggcg gcagcgagcc
     1561 ggcggggccg ctcgccgcgt atccgttgaa gtggaggaat tggtcgacca gcgcggtcgc
     1621 gttggccggc gcgacgacat cgtcgcgatc gccctgcacg acgagaagcg cgacgtgtgg
     1681 cgctgcaccg ttgcgaaccg cgctccgcgc gatgtcggca acacgcgcca cgtcgttgtc
     1741 gggtccgcgc cgcattaccg acagcgcgct cgccggtgac gacgcggcgc cgcacgcgag
     1801 ccccgaatgg acgaggatgc cgcgcacgag gcgcggatgg cgcaggccga gcacggcggc
     1861 aagcgcacct cctgcggaga ggccggcgac gaagacccgc tcgcgccgcg cgcggtagcg
     1921 gcgtcgcacc tgcacgatct gtgcagcgac gatcgcagcc tcacccgcgc cgcgctcggt
     1981 gttcgcctcg aaccagttcc agcagcccca ggcattcgcc gagctggatt gtcgcggcag
     2041 gaggacgagc gtgccgtcgc ggtcggcatg cgacgcgatg cgcgtcagcg cggcgaagtc
     2101 ctcgggtgtc tggttgcatc cgtggcatag cacgcgaagc ggcgcgcgca cgcggcgatt
     2161 ccagccgcga ggcacgtaga cgaggtaatc gcgggcgggt tcgacgcccg gcgcggtggt
     2221 gatgtaaccg cgccacgacg aggcgctgcc ggtttcgaag cggccgggga gctcggcgcg
     2281 gcgcaatcgc gccgcgagcc acgccacgag cgcatgcgcg cgccgcctca gatcacggaa
     2341 gatctgcatc gaaggcattc agcgtgcaga ttggcccgag ccgcgcaaag cgagcgaaac
     2401 cttcgtccgc tatatgagca ccacgtcgta ctgctcctgc gtatacagcg tctcgacctg
     2461 cagcgagatc ggcttgccga tgaaatcgcc gagctgcgca agactctgcg actcctcgtc
     2521 gaggaacatg tcgatcaccg actgcgacgc gaggatacgg aactcgcgcg cgttgaactg
     2581 ctttgcctcg cgcaggatct cgcgcaggat ctcgtagcag atcgtctgcg ccgtcttgaa
     2641 ctcgccgcgg ccgccgcacg tcgggcaggt ctcgcacagc acgtgcgcga gcgactcgcg
     2701 cgtgcgcttg cgcgtcatct cgaccaggcc gagctgcgtg aagccgttga cggtgaggcg
     2761 cgtgcggtcc ttctcgagcg ccttgtcgag ctcggccagc acggcgttgc gatggtcgac
     2821 gttctccatg tcgatgaagt cgatgatgat gatgccgccg aggttgcgca tccgcagctg
     2881 ccgtgcgatc acctgcgcgg cttcgaggtt cgtcttgaag atcgtgtcgt cgaagctgcg
     2941 gccgccgacg aagccgccgg tgttgacgtc gatcgtcgtc atcgcctcgg tttgatcgat
     3001 caccagatag ccgccggact tcaaaggcac ttccttcttc agcgcacgct ggatttcgtc
     3061 ctcgacccca tacaggtcga agatcgggcg ttcgccgggg taatgttcta tgcgctcggc
     3121 caaatcgggc atgaacgtgc gcgcgaa
//
LOCUS       DASSVX010000293         4631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_596626, whole genome shotgun sequence.
ACCESSION   DASSVX010000293 DASSVX010000000
VERSION     DASSVX010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596626"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFO53_12640"
     CDS             complement(<1..573)
                     /locus_tag="VFO53_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8877170.1"
                     /translation="MIDSIRSQPPEGPLYPDCLPGASGMLALDARHTMHWEECGNPDG
                     VPVLFIHGGPGGGSQPHHRRFYDPSFWRIVLYDQRGAGRSTPIADVVDNTTPHLVDDI
                     ERLRIHLGVDRWLLFGGSWGSTLALVYAETHPARVLGLVLRGVFLASRAEVEWFIDGM
                     QRFFPDAWRDFTHFLPRAERADVLHSYYRRL"
     gene            655..1878
                     /locus_tag="VFO53_12645"
     CDS             655..1878
                     /locus_tag="VFO53_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET8877171.1"
                     /translation="MTIDIRSRLTTGDALPADASRALLIGRAFVPAVKGPVLVRVDGD
                     SLFDLSAIAPCASELLNRPGIVHEIRAANALPRVGALADALRNSPDDARDPSLPWLLA
                     PCDLQAVKASGVTFVASMLERVIEEQARGDPARADTVRRKIAGVIGDNLRSVRPGSSE
                     AMRVKDVLIAEGLWSQYLEVGIGPDAELFTKVQPMSSVGTGADVGIHRQSTWNNPEPE
                     IVLAVNARGDTVGAALGNDVNLRDFEGRSALLLGRAKDNNASCAIGPFIRLFDAAFTI
                     DTIRRADLALKVEGADGFVLDGSSSLAQISRDPLDLVGQAIGADHQYPDGLMLFLGTM
                     FAPTQDRHAAGQGFTHDAGDVVTIATPSLGTLANRVNYADAIAPWTFGAGALMRSLAE
                     RGLLAPRATPPSAVP"
     gene            complement(1853..2719)
                     /locus_tag="VFO53_12650"
     CDS             complement(1853..2719)
                     /locus_tag="VFO53_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET8877172.1"
                     /translation="MKLVRYGPAGREKPGLIDADGVLRDLSRKVDDIDGESLSPMSLS
                     GLRKLDTRRLPAVKGRPRLGPCIATPPKFVAIGLNYSDHAKETGAPIPEHPVVFFKAQ
                     TCIVGANDNVMVPLESTQLDWEVELGVVIGRTARYVAEKDALRYVAGYCVVNDVSERE
                     FQMKRSASQWSKGKGCDTFGPVGPWLVTSDEIKDPQNLDMWLDVNGERRQTGNTRTMI
                     FTVARLVADVSRYMTLLPGDLITTGTPPGVALGMKPPQWLKVNDVVTLGIAGLGEQRQ
                     RIVPFKGRRMAA"
     gene            complement(2741..3496)
                     /locus_tag="VFO53_12655"
     CDS             complement(2741..3496)
                     /locus_tag="VFO53_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8877173.1"
                     /translation="MNGRLAGRTAFITAAGQGIGHATALAMAREGASVIATDVNPALL
                     APMQGVANVTTRTLDVLDDEAVSRTLRELPPLSILFNCAGYVHNGTILDCKPEDWAFS
                     FNLNARAMYVAIRAALPAMLARHEASGQGASIINMASIAGSIKGLPNRFVYGASKAAV
                     IGLTKSVAADYVQHGIRCNAIAPGTVDTPSLAERINAYPDRVEARRMFIARQPMGRLA
                     KPEEIAPLVVFLASDEAAFVTGNVYSCDGGMTI"
     gene            complement(3568..4311)
                     /locus_tag="VFO53_12660"
     CDS             complement(3568..4311)
                     /locus_tag="VFO53_12660"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HET8877174.1"
                     /translation="MAAPALRTQSLSIAPGVSGYLAKPSGVPGIGVVVMMEAFGLNAF
                     VKGVCDRFARAGYMALAPDIYHGDTFDYADRERAIARLGEVDDNVAMREVGAALDMLT
                     AQGARDGRLAIIGFCMGGRLAFLANAVHGDRLAASVSFYGGGIAPAVPRGKRKSLIDR
                     VSDVSAPQLLIYGARDASIASDEHARLAQALSDANKRYTLAVLPDAAHAFATFDRESY
                     HAPAAEAAWRIAFAFLVDTFMQGSAAAYA"
     gene            4537..>4631
                     /locus_tag="VFO53_12665"
     CDS             4537..>4631
                     /locus_tag="VFO53_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8877175.1"
                     /translation="MPFQSIEPRRLYRQIADQVRMLIRNGEFAAGA"
BASE COUNT          717 a         1552 c         1614 g          748 t
ORIGIN      
        1 caggcgccgg taatagctgt ggagaacgtc ggcacgctcg gcccgtggca gaaagtgcgt
       61 gaaatcgcgc caggcgtcgg ggaagaagcg ctgcatgcca tcgatgaacc attcgacctc
      121 ggcgcggctt gcgaggaaca cgccgcgcag cacgagcccg agcacgcgcg ccggatgcgt
      181 ctcggcatag acgagcgcga gcgtcgagcc ccacgagccg ccgaacagca gccagcggtc
      241 gacgccaagg tggatgcgca gccgctcgat gtcgtcgacg agatgcgggg tcgtgttgtc
      301 gacgacgtcg gcgatcggcg tcgagcggcc ggcgccgcgc tggtcgtaga gcacgatgcg
      361 ccagaacgac ggatcgtaga agcggcggtg atgcggctgg ctgccgccgc cgggtccgcc
      421 gtggatgaag agcaccggca cgccgtcggg gttgccgcat tcctcccagt gcatcgtgtg
      481 ccgcgcgtcg agtgcgagca tgccgctcgc gccgggcagg caatcgggat aaagcggacc
      541 ttcgggcggc tggctgcgaa tcgagtcgat catcgggcag ggcacgacaa gcgcggatgc
      601 gcgctacgca aacgggcaac gcggttcgcc cgagtgcccg tatgatcgca cctcatgaca
      661 atcgatatcc gttcacgcct gactaccggt gatgcgctcc cggccgacgc gtcgcgcgcg
      721 ctgctgatcg gccgcgcctt cgtgccggcc gtgaaggggc ccgtgctcgt gcgcgtcgac
      781 ggcgacagcc tgttcgatct gtccgcgatc gcgccttgcg caagcgagct tttgaaccgg
      841 cccggcattg tgcacgagat ccgcgccgcg aatgcgctgc cgcgcgttgg tgcgctcgcc
      901 gatgcgctgc gcaattcgcc cgacgacgcg cgcgatccgt cgctgccgtg gctgcttgcg
      961 ccgtgcgacc tgcaggcggt gaaggcgtcc ggcgtcacat tcgtcgcgag catgctcgag
     1021 cgggtgatcg aggagcaggc gcgtggcgat ccggcccggg ccgacaccgt gcggcgcaag
     1081 atcgccggcg tgatcggcga caacctgcgc agcgtgcgtc cgggctcgtc cgaagcgatg
     1141 cgcgtcaagg acgtcctgat cgcggaagga ttgtggtcgc aatatctcga agtcggcatc
     1201 ggacccgacg cggagctctt caccaaggtg cagccgatgt cgtcggtcgg gacgggcgcg
     1261 gacgtcggca ttcaccggca gtcgacgtgg aacaatcccg agcccgagat cgtgcttgcc
     1321 gtgaacgcgc gcggcgacac cgtgggtgca gcgctcggca acgacgtcaa cctgcgcgac
     1381 ttcgaagggc gcagcgcgct gctgctcggg cgggcgaagg acaacaacgc gtcatgcgcg
     1441 atcgggccct tcattcgcct gttcgacgct gcgttcacca tcgacacgat ccgccgcgcc
     1501 gatctcgcgc tgaaagtcga gggcgccgat ggcttcgtgc tcgatggctc gagctcgctt
     1561 gcgcaaatca gccgcgaccc gctcgacctc gtcgggcagg ccatcggcgc cgatcaccag
     1621 tatcccgatg ggttgatgct gttcctcggc acgatgttcg cgcccacgca ggatcgtcat
     1681 gcggccggcc agggcttcac gcacgacgcc ggcgatgtcg tgacgatcgc aacaccgtcg
     1741 ctcgggacgc tggccaaccg cgtgaactac gccgacgcca tcgcgccgtg gacgttcggc
     1801 gccggtgcgc tgatgcgcag cctcgccgag cgcggcctgt tggcgccgcg ggctacgccg
     1861 ccatccgccg tcccttgaac ggcacgatgc gctggcgctg ctcgccgagg cccgcgatgc
     1921 cgagcgtcac gacgtcgttc accttgagcc actgcggcgg cttcatgccg agcgccacgc
     1981 cgggcggcgt gccggtggtg atgaggtcgc ccggcagcag cgtcatgtag cgcgacacgt
     2041 cggcgacgag ccgcgcgacg gtgaagatca tcgtgcgcgt gttgccggtc tggcggcgct
     2101 cgccgttcac gtcgagccac atgtcgaggt tctgcgggtc cttgatctcg tcgctcgtca
     2161 cgagccacgg cccgacggga ccgaacgtgt cgcagccctt gcccttgctc cattgcgacg
     2221 cgctgcgctt catctggaac tcgcgctccg acacgtcgtt caccacgcag tagccggcga
     2281 cgtagcgcag cgcgtccttc tcggcgacgt agcgcgcggt gcggccgatg acgacgccga
     2341 gctccacctc ccagtcgagc tgcgtcgatt cgagcgggac catcacgttg tcgttcgcgc
     2401 cgacgatgca cgtctgcgcc ttgaagaaca cgaccggatg ctcgggaatc ggcgcgccgg
     2461 tttccttcgc atgatcggag tagttgagtc cgatcgcgac gaacttcggc ggcgtcgcga
     2521 tgcacggtcc gaggcgcgga cggcccttca ccgcgggtag ccgccgcgtg tcgagcttgc
     2581 gcagccccga cagcgacatc ggcgaaagcg attcaccatc gatgtcgtcg accttgcgcg
     2641 acaggtcgcg cagcacgccg tcggcgtcga tcagtcccgg cttttcgcgc cccgcggggc
     2701 cgtaacgtac gagcttcatg ccttcgtctc ctcctgttgt tcagatcgtc atgccgccgt
     2761 cgcaggaata cacgtttccc gtgacgaatg ccgcttcgtc ggatgcgagg aacacgacga
     2821 gcggcgcgat ttcctcgggt ttcgcaagcc gtcccatcgg ctggcgcgcg atgaacatcc
     2881 ggcgggcctc gacgcgatcg ggatacgcgt tgatgcgctc ggcgagcgac ggcgtgtcaa
     2941 cggtgccggg cgcgatcgca ttgcagcgaa tgccgtgctg cacatagtct gcggccaccg
     3001 atttcgtgag gccgatcacc gcggccttcg acgcgccgta cacgaaccgg ttcggcaatc
     3061 ccttgatcga cccggcgatg gaagccatgt tgatgatgct ggcgccctgt ccgctcgcct
     3121 cgtggcgagc aagcatcgcg ggcagcgctg cgcggatcgc gacatacatt gcgcgggcgt
     3181 tgaggttgaa gctgaacgcc cagtcctccg gcttgcaatc gaggatcgtg ccgttgtgca
     3241 cgtagcccgc gcaattgaaa agaatcgaaa gcggcggcag ctcgcgcagc gtgcgcgaca
     3301 cggcctcgtc gtcgaggacg tcgagcgttc gcgtcgtgac gttggcgacg ccctgcatcg
     3361 gtgcgagcaa cgcggggttg acgtcggtcg cgatgacgct ggcgccctcg cgcgccatcg
     3421 cgagggccgt tgcgtgtccg atgccctgtc cggccgcggt gatgaaggcg gtcctgcctg
     3481 cgagtcgtcc gttcatggct cgtgcgcgcg aaacggcgga ttctacgcca tgcgcgcgcg
     3541 gcacgagccg cgcgcaccgc gcgagcttca cgcgtacgcg gcggccgagc cctgcatgaa
     3601 cgtgtcgacc agaaatgcga aggcgatgcg ccatgccgct tcggcggccg gtgcatggta
     3661 gctctcgcgg tcgaacgtcg cgaatgcatg cgctgcgtcg ggcagcacgg caagcgtgta
     3721 gcgcttgttc gcgtcggaca gggcctgcgc aagccgcgcg tgctcgtcgc ttgcgatcga
     3781 cgcatcgcgc gcgccgtaga tgagcagctg cggcgccgac acgtcggaca cgcggtcgat
     3841 cagcgacttg cgcttgccgc gcggcacggc gggcgcaatg ccgccgccat agaacgagac
     3901 ggaggcggca agccggtcgc cgtgcacggc gttggcgaga aaggcgagcc gcccgcccat
     3961 gcagaacccg atgatcgcga gccgcccgtc gcgggccccc tgcgcggtca gcatgtcgag
     4021 cgcggcgccc acttcgcgca tcgcgacgtt gtcgtcgact tcgccgagcc gtgcaatggc
     4081 gcgctcgcgg tcggcatagt cgaacgtgtc gccgtggtag atgtcggggg cgagcgccat
     4141 gtagccggcg cgcgcgaagc ggtcgcatac gcccttgacg aacgcgttga ggccgaaggc
     4201 ctccatcatg acgacgacgc cgatgccggg cacgccgctc ggcttcgcga ggtagcccga
     4261 aacgccgggt gcgatcgaca gggattgggt acgcagcgcg ggcgcggcca tgatcggatt
     4321 cgaagctgcc tttttgcgtc gtcggatcgc aacgcgaaga atgcgacgcg caacggtccc
     4381 gcgcaagcgc tccagcgtcc gccgggcggc gcacctccgc ccctgttgcg gttatgccgc
     4441 gttgcggccg attggtcatg ccacttgacc ggtgaagcga cacgcgggta gccttgccga
     4501 ttgccttgcg gcgccggcag cggtgccgtt tcgaccatgc ccttccagag catcgagccg
     4561 cgccggctct accggcagat cgccgaccag gtccgcatgc tgatcagaaa cggggaattc
     4621 gctgcgggcg c
//
LOCUS       DASSVX010000294        17076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1129514, whole genome shotgun sequence.
ACCESSION   DASSVX010000294 DASSVX010000000
VERSION     DASSVX010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 17076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17076
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129514"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..783
                     /locus_tag="VFO53_12670"
     CDS             1..783
                     /locus_tag="VFO53_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HET8877176.1"
                     /translation="MLAACALAAPALAADVGVSVTMGQPGFYGRIDVGGVAPPRLLYR
                     EPVVIAPAPGRVVRAPIYLRVPPGHAKNWRKFCARYNACGQPVYFVQDSWYNDVYVPY
                     YRRGHPVIQPAPIYAEPARPRLYTIPVESVHAVVGPPEQRCWVERQQVAERGDVNVPG
                     AIAGAVIGGLLGHQVGGGRGRDVATAGGAVAGAAIGANVGRDGGQVVTRDVQRCRTVQ
                     RNPHPDYWDVTYRFRGQLHRAQFRAPPGRTIVVDDTGVPQMQ"
     gene            complement(797..2212)
                     /locus_tag="VFO53_12675"
     CDS             complement(797..2212)
                     /locus_tag="VFO53_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HET8877177.1"
                     /translation="MPVRAPKTSVWNWLGRRKTVPLQAPVSRFAVARVRRIFGECASG
                     IGGEASARSRVAMLATIFRGLDDDGKRAFLALLATYVNGDEKSAAPLVQEYARSDGIA
                     HQLAALRLRDALAGTSLRILRQFNLLEDGVKFLVDLRADAIRLKSGSPELQVIDEELL
                     SLFRDWFALGNLELRRISWNSPAALLERLMTYEAVHEIQSWADLRHRLESDRRCYAFF
                     HPRMPDEPLVFVEIALTDRVASSVQPLLDESTPVTDPRAADAAVFYSISSTQPGLRGV
                     SFGGFLIKRVVESLLAEYPKLTTFATLSPVPGFRRWLDAHLQSGTASLLTERNRGAFA
                     KLARGRDAAGELRRALAGDTTADRATNAALEAVLVPLCARYLVIEKRNAMPVDPVARF
                     HFGNGARLDRINWLANTSTRGLQESLGIMVNYVYEPAEIEENHEAYYRDGKVAVSPGV
                     RKLLRRGGNGTATEGIAAPAP"
     gene            complement(2258..2998)
                     /locus_tag="VFO53_12680"
     CDS             complement(2258..2998)
                     /locus_tag="VFO53_12680"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005891048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET8877178.1"
                     /translation="MIRAGAAAIAPSTRGGFIAGMQTGISHLVPQDGGTLDCERLSEV
                     AHAARDMRFNDGRCDRQGRFWAGTMAIDMALAANVGSLYRFDDRRGLARTFGGFVVPN
                     GLAFSPDGRTMYLSDSHPSVQLIWQFDYDVDSATPANRRLYVDMNTLPGRPDGAAIDE
                     DGCYWICANDAGLVHRLTPGGTLDRSLPLPVKKPSMCAFGGANLDTLFVTSIRPAGID
                     LADQPLAGGVFALHPGVRGVEEPRFGNH"
     gene            complement(2995..3447)
                     /locus_tag="VFO53_12685"
     CDS             complement(2995..3447)
                     /locus_tag="VFO53_12685"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HET8877179.1"
                     /translation="MRRLLVVAWLLAATAAFADDALDARLKLLETQLRCLVCQNETLA
                     ESAAPLAEDLRTEVRELAVAGKSDDDIRAYLVARYGDFVLYKPPMKPVTWLLWFGPFV
                     LLFGGLVAWLFVLRRRARMKRSDGDDANTPAHADAGVEVARARELLDE"
     gene            complement(3447..3974)
                     /locus_tag="VFO53_12690"
     CDS             complement(3447..3974)
                     /locus_tag="VFO53_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HET8877180.1"
                     /translation="MLRWSLPLAVFLVLLAFLFVGLFRDPREVPSPLVDKPAPSFSLA
                     KLHQPGERLSNADMRGQVWLLNVWASWCVSCRVEHPLLVALAKADVVPVIGLAYKDKP
                     EDGLAWLASNGDPYRMSIVDRDGRVGIDFGVYGVPETFVIDKAGIIRYKQIGPLTADA
                     LDKTILPLVRELQRS"
     gene            complement(3988..5976)
                     /locus_tag="VFO53_12695"
     CDS             complement(3988..5976)
                     /locus_tag="VFO53_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HET8877181.1"
                     /translation="MIPEIGHFALILALLVAIVQGVMPMLGAARRDATLMSVAVPAAR
                     AQFALVALAFGCLAYAFVTSDFSVANVAEHSNSHLPAHYRFAATWGSHEGSLLLWSLM
                     LAGWGCAVTIFSRHLPQTLRARVLAVMGLVAVGFLLFLLATSNPFARLSPVPAEGSDL
                     NPLLQDPGMVVHPPMLYMGYVGFSVAFAFAIAALIGGRLDAAWARWSRPWTTVAWAFL
                     TMGIMLGSVWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGAFRSWTV
                     LLAIVAFSLSLVGTFLVRSGVLTSVHAFATDPARGVFILAFLAIVIGGSLALYALRAG
                     AVGAGGGFAMVSREAMLLGNNILLIVAVASVLLGTLYPLMIDALHLGKISVGPPYFNA
                     VFYPLMAPLVFLLGVGPMAAWRQARVPDLWTKLQWALYVALATGALAPFAMGQWKPLV
                     AIGLLMAAWIAAATVTAFVQRLRSAPQRGLADKLRANTSAWYGMLLAHLGVAVFITGV
                     TLVKGYEVERDVRLDVGQSVEVAGDTYTFRGVAPRAGPNFDATVATVDVTRDGRNIAT
                     LTPQKRAYRASGQVMTEASIQSGIGGDRYVSLGEPVTEQGAAGAWGVRIYVKPFVDWI
                     WGGCLLMALGGFFAVADRRYRLAVRQRFGAAIAAVSGA"
     gene            complement(6089..6556)
                     /gene="ccmE"
                     /locus_tag="VFO53_12700"
     CDS             complement(6089..6556)
                     /gene="ccmE"
                     /locus_tag="VFO53_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET8877182.1"
                     /translation="MKPRHRRFAWIGAGLVALGVAVALVLNAFQSNLVFFFTPSQVVA
                     KEAPQSRPFRIGGLVEQGSLTRDPNSLTVHFRVTDTAQTIPVTYTGLLPDLFKEGKGV
                     VAQGTLGSDGAFHATEVLAKHDENYMPPAAKDAIDQAHRKRAMTMSSMSEPQR"
     gene            complement(6553..6744)
                     /gene="ccmD"
                     /locus_tag="VFO53_12705"
     CDS             complement(6553..6744)
                     /gene="ccmD"
                     /locus_tag="VFO53_12705"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HET8877183.1"
                     /translation="MAAFFAMGGYAWYVWMAYGVAALAIAVEIAAVRARRKRAFDELR
                     MSVDAQPAGATMTRKGSPR"
     gene            complement(6744..7493)
                     /gene="ccmC"
                     /locus_tag="VFO53_12710"
     CDS             complement(6744..7493)
                     /gene="ccmC"
                     /locus_tag="VFO53_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HET8877184.1"
                     /translation="MINWFRFASPISFYPIAGSWSYAFGALAALLAGAGIVIALWIAP
                     TDATQGDAYRIIFIHVPAAWMAMFTYLVMAGWSALALGFNTRLSAMMAQALAPTGALM
                     AALALWTGAFWGRPTWGTYWAWDARMTSTLILLFLYLGIIALRAALEDPRRADRACAV
                     LALVGAVNVPIIYFSVQWWNTLHQGSSISLTAAPKMAMTMLAGILVMTAAAWSYTIAV
                     VLARVQAIIVERERGAGWLESLIRFPAGENA"
     gene            7843..11118
                     /locus_tag="VFO53_12715"
     CDS             7843..11118
                     /locus_tag="VFO53_12715"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET8877185.1"
                     /translation="MLVLAVLFPSAAQAGTDVQTGLGGNEPTTAAVNPRSPSVIAVAR
                     GLSVAISTDFGRTFPTVANMPQGGGGVPATYGTIATWNFCGDGSVTFDSQGRLFMTYL
                     LCGNDNTVPTPNRVDVSSFVQQVNPTTGALIGNPVEVTGANALNNDDKEWIAADANPD
                     SPFRDNLYVVWSRLNTSQIMFSRSTDGGLNWSAPTAISAAGEGFVWPSHVAVAPNGDV
                     YVSYHGDTCGAATAQMFVLRDSNGGGQFQAGTGFQKTSFASAVSCNRRDASASIPNAQ
                     FWMQGANQGFVIPDPIRPGNVYVVVNDDPQDNFTATTGDHADVKLARSTDNGNTWTLS
                     TISHAPPGTLQVFPTGASDQLGNLVITWWDTRRGFTNAAGNLLLEVYATVSRDGGVTF
                     TNDFRVSDLPFDPDLNASCRFGPAATCGTVTGAGTASTLRIGEYNGVAAADGTGYVIW
                     SGNQTPPNPQAVPPTAAAGGQTTYFDVFTILGAFPDAAEPNDAWDPGVASVLGANRTY
                     QNQDLTIHSSTDEDWFKVTALATGQLFFTIDYNSRFADLDLQVRDKFNNPVATQTLGL
                     DVNNTESIFAPAVAGEAYFVRVFPEPGQAPSLNTYDLGIVNTAAPVPFGIVLAPGSDS
                     GSSGSDNITKIATPTIRLAVDLDPLAPLAFSPDDATPTLTDDAPGYKVEVYRNGVTAG
                     RATPVAGQPGIFEFTFTSPLAEGQNFLTARVVIVDNSDNAAVAGTLHAVGFGGESQTV
                     VVTLDTTAPTGASLGPLDLLASSDSGGISDDNITTFSTPSFGLQVNEPGFIRIYAATV
                     PGGIPIQVGQIQATTAGAWQATVQSLADGVYDMTATIEDAAGNVGPPTSALRVTIAKN
                     SLTLPGYTVGGAGGPVTIDLAARTISGFASASPSLLIGIAGIPSVNVDMNGMTLTVNL
                     TNGNDALSYTPSGPGSGSIALAGVNQVLNFTGSGMFTVNPLGGNDTVTTYGSSVGDAV
                     KVEVDTTVAVQVGSALTLNMPASQIEKVGVSTLQGNDTINVNIHDTVNASLFVDGGDP
                     TTVNKGNDVLNLIDASATKKGQYSNISGGSTSGSGAVVLKFKSAGTATRIDYVNIEKQ
                     TRK"
     gene            11244..11816
                     /locus_tag="VFO53_12720"
     CDS             11244..11816
                     /locus_tag="VFO53_12720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HET8877186.1"
                     /translation="MSDAATSAPATITLLIDRANSGNAEALNALFATLYPELHALAHA
                     RIRRNRQLTLLDTTALLHESYLRFVRLGQLNVGNRAHFLAYAARTMRSIVVDFARARL
                     AQRRGGGGDDAILDTDAALAGTGEEEVIRVHEALDELRHADERLVRVVEMRYFGGLTE
                     DEIGSALGITDRTVRRMWDKARVMLEVALR"
     gene            11852..14725
                     /locus_tag="VFO53_12725"
     CDS             11852..14725
                     /locus_tag="VFO53_12725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET8877187.1"
                     /translation="MKLEPAQWRALSVLLDEALELEPTGRRAWLDALSGEARGFQPML
                     ADLLVRTEAGAEMFLDTLPRIAAPDRTPAFFAGGRVGPYRLLRRLGRGGMGEVWLSER
                     ADGVLKRPVALKLPLLATNPDILAERFAREREILASLAHAHIARLYDAGFDIEGQPYL
                     ALEYIDGIPLTHYADVHRLGVLARLRLFDQVLDAVAHAHASLVLHRDLKPSNILVTPA
                     GDVKLLDFGIAKLLQSGAAEDSALTRISGQALTPDYASPEQITGGPLTTAADVYGLGV
                     VLYELLTGKRPYRLKRGSPAELEEAIAFVDPAAPSRARIVDAAAAQRATTVHRLRRTL
                     SGDLDTIVLKALRKRPDERYAGARALAEDLERYRDGAPVLARPTSVSYRTAKWMKRHR
                     AGVALGSVVAATLLGATGIALHQARVASIEAQRAEASNDFLMGLFQHAARNNPGGAAA
                     ADTTLRQLLAIGSRKVLDDSRASPELQLDVSALLARLNLEVDLVDTAERLTERSLVLA
                     RKLHGENSLPVAQVLAQHADNLYRSGRYAEAIEASRQVLAHVDRARAPADELRARAHI
                     LIGNSMFQVDASKTAEPLQHLETALELLKRAHATTEDRSRAAYFIGWIHESTGDFARA
                     ETYYNDGIEAGRANFGERSFIVAFGYEGLAESLRRQRRLAEARDTMGRAIDIYQYVLA
                     PRHATVAFARTSLSMIEASSGRLAEAERLADDALAVTKTVFGEHARQTVVPATQAARL
                     KVQRGELESGLALYEDALAALVAEDRSSLTNRALRIEYAEALLETGEIGKARTALDEA
                     QAGFEAAHDLASVRGAWLRLVRGELAVAAGDSDAASAFDHVLDSFTRSDQGAPALARR
                     AANATARYSPTPERAETMLQKLPEFGLLPTSPDELDIDIVDKAQLADSIGRLFNVAGK
                     RPEAFAWLSKAVALREQLEVPESPRLRDARLALTRAGSVNERR"
     gene            complement(14774..15529)
                     /gene="ccmB"
                     /locus_tag="VFO53_12730"
     CDS             complement(14774..15529)
                     /gene="ccmB"
                     /locus_tag="VFO53_12730"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET8877188.1"
                     /translation="MSVMKPSPLAETLAVQPAPAESSADAHASVLAAVGWALARDFRV
                     ALRSKGELGVQLLFYVIVVSLFPLATSPSRETLATLGPGVLWTSALLASLLSLPRLFA
                     SDYHDGTLEQIALSPRPLAALVSGKMAAHWLTSGLPVALMAPLLGAQYALDAPVLATL
                     AASLLLGTPILSLLGAVGAALTLGARGGGALLALLVLPLYAPVLIFGAGAVDAVRAGV
                     SAAPQLSLLGAGLLLALVAVPFAVAAAVRIALD"
     gene            complement(15591..16229)
                     /gene="ccmA"
                     /locus_tag="VFO53_12735"
     CDS             complement(15591..16229)
                     /gene="ccmA"
                     /locus_tag="VFO53_12735"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HET8877189.1"
                     /translation="MSTQNSIEARRLAAQRGYARLFDGVDFSLQAGEALVVSGRNGSG
                     KTTLLRIVAGLTAPAEGELRWRGERVAAFDPRLRAAVAFNGHASALKDELTAEENLDA
                     WIALDAPRASRESIAGALDAVALLKQRRLPVRVLSQGQRRRVGLARLALVRRALWILD
                     EPLTALDADGAGMLGALLDAHLAQGGLCIAASHQPLPIAPDRVRRLELGAPT"
     gene            complement(16241..>17076)
                     /locus_tag="VFO53_12740"
     CDS             complement(16241..>17076)
                     /locus_tag="VFO53_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8877190.1"
                     /translation="EHPEDPTGWRLLARAYAALGRFPEAVAAFKEAAERGPADATLLA
                     DWADALAMHNQSLQGEPSQLIERALAIDPRQPKALSLAATAALERKDYGVAIDEWRKL
                     KAQFAPGSDEAKQIDSMIAEANAARQGGAMSPGGKGGAMSPGAKGGGNDAAAGAATTA
                     NAGDTAITGRVSLDPKLRGRASANDTLFIFARAVNGPRMPLAVLRATAGELPRNFTLD
                     DSMAMAPASRLSGASDVVVEARISKTGSATPSPGDLQGNSTVVKPGAQDVSIVIDDVV
                     R"
BASE COUNT         2979 a         5815 c         5666 g         2616 t
ORIGIN      
        1 gtgctggccg cctgcgcact tgccgcgccg gcacttgccg cggatgtcgg cgtgtcggtg
       61 accatgggtc agcccgggtt ttacgggcgc atcgatgtag gtggcgtcgc gccgccgcgc
      121 ctgctgtacc gggagccggt cgtcatcgcg ccggcacccg gtcgcgtcgt gcgcgctccc
      181 atctatcttc gcgtgccgcc gggtcacgcg aagaactggc gcaagttctg cgcgcgctac
      241 aacgcttgcg gtcagccggt ctatttcgtt caggacagct ggtacaacga cgtgtacgtg
      301 ccgtactacc ggcgcggcca tcccgtcatc cagcctgcac cgatctatgc cgagcccgca
      361 cggccgcggc tttacacgat tccggtggag tcggtgcacg cggtcgtcgg accgcccgag
      421 cagcgctgct gggtcgaacg tcagcaagtg gccgagcgcg gcgacgtaaa cgttcccggc
      481 gccatcgccg gcgccgtgat cggcggcttg ctgggccatc aggtcggcgg cggccgcggc
      541 cgcgatgtcg ccacggcagg cggtgcggtg gcgggggcgg cgatcggcgc gaatgtcggt
      601 cgcgatggcg ggcaggtggt gacgcgcgac gtgcagcgct gccgcacggt ccagcgcaac
      661 ccgcatcccg attactggga cgtcacgtac cgctttcgcg gacagttgca tcgcgcgcag
      721 tttcgcgcgc cgcccggacg cacgatcgtc gtcgacgaca ccggtgttcc gcaaatgcaa
      781 tagaaggcgc ggttcgctac ggcgcaggcg cggcgatgcc ctcggttgcc gtgccgttgc
      841 cgccgcggcg cagcagcttg cgtaccccgg gcgacacggc gaccttgccg tcgcggtaat
      901 acgcctcgtg gttttcctcg atctcggccg gctcgtacac gtagttgacc atgatgccga
      961 gcgattcctg caggccgcgc gtcgatgtgt tcgcgagcca gttgatgcgg tcgagccgcg
     1021 cgccgttgcc gaagtggaag cgcgcgaccg ggtcgaccgg catggcgttg cgcttctcga
     1081 tgacgagata acgcgcacaa agcggcacga gcaccgcctc gagggcggca ttcgttgcac
     1141 gatcggcggt ggtgtcgcca gcgagcgcgc gtcgcagctc gccggcggcg tcgcggcccc
     1201 gtgcgagctt ggcgaacgcg ccgcgattgc gctcggtgag aagcgaggcc gtgcccgatt
     1261 ggagatgcgc gtcgagccag cggcgaaagc cgggtaccgg cgacagcgtc gcgaatgtcg
     1321 tgagcttcgg atattcggcg agcaggctct cgacgacgcg cttgatcagg aagccgccga
     1381 acgacacgcc gcgaaggccc ggctgcgtgc tcgatatcga gtagaagacc gcggcatcgg
     1441 ccgcgcgcgg atcggtcacc ggcgtcgatt cgtcgagcag cggctggacg ctcgacgcga
     1501 cgcgatcggt gagcgcgatc tcgacgaaca cgagcggctc gtcgggcatt cgcggatgga
     1561 agaacgcata gcagcggcgg tcggattcga gccggtgacg aagatcggcc caggactgga
     1621 tctcgtgcac cgcctcgtac gtcatcagcc gctcgagcag cgccgccggc gagttccacg
     1681 agatgcggcg cagctcgaga ttgccgagcg cgaaccagtc gcgaaacagc gacagcagct
     1741 cctcgtcgat gacctgcagc tccgggctgc cggacttgag ccggatcgca tcggcgcgaa
     1801 gatcgacgag gaacttgacg ccatcctcga gcaggttgaa ttgacggagg atgcgaagcg
     1861 acgtgcccgc cagcgcgtcg cgcaggcgca gggcggcaag ctggtgcgca ataccgtcgc
     1921 ttcgcgcgta ttcctgcacg agcggggccg ccgacttctc gtcgccattc acgtatgtcg
     1981 cgagcagcgc gagaaacgcg cgcttgccgt cgtcgtcgag gccgcgaaag atcgtcgcga
     2041 gcatcgccac gcgcgagcgt gccgaagcct cgccgccgat gccactcgcg cactcgccga
     2101 aaatgcgccg cacgcgcgca acggcaaagc gcgacaccgg tgcttgaagg ggaaccgttt
     2161 tgcggcgtcc cagccagttc cacacgctgg tcttcggagc gcgcaccggc acgggatcat
     2221 tcgaaaaggg ggtcatttgc atgctgcgtg aaggcggtta gtgattgccg aaacgcggct
     2281 cctcgacgcc gcgcacgccg ggatgcagcg cgaacacgcc gccggcgagc ggttgatcgg
     2341 caaggtcgat tcccgcgggc cggatcgagg tgacgaacag cgtatcgagg ttggcgccgc
     2401 cgaacgcgca catcgaaggc ttcttcacgg gcagcgggag cgagcggtcg agcgtgccgc
     2461 cgggtgtcag gcgatgcacg agaccggcgt cgttggcgca aatccaatag cagccgtcct
     2521 cgtcgattgc ggcaccgtcg gggcgtccgg ggagcgtgtt catgtcgaca tacaggcgcc
     2581 ggttcgcagg tgttgccgag tcgacgtcat agtcgaattg ccagatcagt tgcaccgacg
     2641 gatgcgaatc ggacagatac attgtgcggc cgtcgggact gaatgccaac ccgttcggaa
     2701 cgacaaagcc gccaaaggtt cgcgcaagac cgcgtcggtc gtcgaaacga tacaggctgc
     2761 ccacgttcgc agcgagcgcc atgtcgatgg ccatcgttcc cgcccagaag cgaccctgcc
     2821 ggtcgcaccg gccatcgttg aagcgcatgt cgcgcgcggc gtgcgcgacc tctgacaggc
     2881 gttcgcaatc gagcgttccg ccatcctgcg gcaccaggtg ggagatgccg gtctgcatcc
     2941 cggcaatgaa gccgccgcgc gtcgaaggcg cgatcgctgc cgcgccggcg cgtatcactc
     3001 gtcgagcagc tcgcgggcgc gtgcaacctc gacgcccgca tcggcatggg cgggggtgtt
     3061 cgcgtcgtcg ccgtccgacc gcttcatccg cgcgcgccgc cgcagcacga agagccaggc
     3121 gacgaggccg ccgaacagca gcacgaacgg gccgaaccac agcagccagg tcaccggctt
     3181 catcggcggc ttgtagagca cgaaatcgcc gtagcgcgcg acgaggtagg cgcgaatgtc
     3241 gtcgtcgctc ttgccggcga cggcgagctc gcgcacttcc gtgcgcaggt cctccgcgag
     3301 cggtgcggca ctttcggcga gcgtctcgtt ctggcatacg aggcagcgca gctgcgtctc
     3361 gagcaacttc aggcgcgcgt cgagcgcgtc gtcggcgaac gctgccgtcg cggcgagcag
     3421 ccacgcgacg acgagcagcc ggcgcatcac gatcgttgca gctcgcgcac gagcggcagg
     3481 atcgtcttgt cgagcgcgtc ggcggtgagc gggccgatct gcttgtagcg aatgatgccg
     3541 gccttgtcga tgacgaacgt ctccggcacc ccgtagacgc cgaagtcgat gccgacgcgc
     3601 ccgtcgcgat cgacgatcga catccggtac ggatcgccgt tcgacgcgag ccaggcgagg
     3661 ccgtcctcgg gcttgtcctt gtacgcgagg ccgatcaccg gaacgacgtc ggccttcgca
     3721 agcgcgacga gcagcggatg ctcgacgcgg cacgacacgc accacgacgc ccacacgttc
     3781 agcagccaca cctgtccgcg catgtcggca ttgctgagcc gctcgccggg ctgatgcagc
     3841 ttcgccagcg aaaaagacgg cgccggcttg tcgaccagcg gcgacggcac ttcccgcgga
     3901 tcgcgaaaga gaccgacgaa caggaacgcg agcagcacga ggaacaccgc gagcggcagc
     3961 gaccagcgca gcactctcat cattttctta cgcgcccgac acggcggcga tcgccgcgcc
     4021 gaagcgctgg cgtaccgcga ggcgatagcg gcgatcggcg accgcgaaga atccgccgag
     4081 cgccatcagc agacaaccgc cccagatcca gtcgacgaat ggcttgacgt agatgcgcac
     4141 accccatgca ccggccgcgc cctgctcggt gacaggctcg cccagcgaca cgtagcgatc
     4201 gccgccgatg cccgactgga tcgacgcctc ggtcatcacc tgccccgagg cgcggtaggc
     4261 gcgcttctgc ggcgtgagcg tcgcaatgtt tcttccgtca cgcgtcacgt cgacggtggc
     4321 caccgtggca tcgaaattgg gaccggcgcg cggcgccacg ccgcgaaacg tgtacgtgtc
     4381 gcccgccact tcgacgctct ggcccacgtc gagacgcacg tcgcgctcga cttcgtaacc
     4441 cttgacgagc gtgacgccgg tgatgaatac cgcgacgccg aggtgcgcaa gcagcatccc
     4501 gtaccacgcc gacgtattcg cacgcagctt gtcggcgagg ccgcgctgcg gcgcactgcg
     4561 cagccgctgc acgaatgccg tgaccgtcgc cgccgcgatc cacgccgcca tcagcagccc
     4621 gatggcgacg agcggcttcc actgtcccat cgcgaacggt gcgagcgcgc cggtggctag
     4681 cgccacgtac agcgcccact ggagcttcgt ccacaggtcg ggcacgcgcg cctgccgcca
     4741 cgcggccatc ggtcccacgc cgagcaggaa gacgagcggc gccatcaacg gatagaacac
     4801 cgcattgaag tagggcgggc cgaccgagat cttgccgaga tgcagcgcgt cgatcatcag
     4861 cggatacagc gtgccgagca gcacgctcgc caccgcgacg atcagcagga tgttgttgcc
     4921 gagcagcatc gcctcgcgcg acaccattgc aaatccgccg ccggcgccca ccgcgcccgc
     4981 gcgcagcgcg taaagcgcaa gcgagccgcc gatcacgatg gcgaggaagg cgaggatgaa
     5041 aacgccgcgc gcgggatcgg tggcgaacgc atgcaccgac gtcagcacgc ccgagcggac
     5101 gagaaacgtg ccgacgagcg acagcgagaa cgcgacaatc gcaagcagca ccgtccagct
     5161 ccggaacgcg ccgcgctttt cggtcacggc gagcgaatgg atgagcgcgg tgccgacgag
     5221 ccacggcatg aacgacgcgt tctcgaccgg atcccagaac caccagccgc cccagccgag
     5281 ctcgtaatac gcccatacgc tgcccagcat gatgcccatc gtcaggaacg cccacgcgac
     5341 ggtggtccac ggtcgcgacc agcgcgccca cgccgcgtcg aggcggccgc cgatcagcgc
     5401 ggcgatcgcg aacgcgaatg cgaccgagaa gccgacatag cccatgtaaa gcatcggcgg
     5461 atgcaccacc atgccgggat cctgcagcag cggattgaga tcgctgcctt ccgccggaac
     5521 cggtgaaagc cgcgcgaacg gattggacgt tgcaagcagg aacagcagga aaccgacggc
     5581 gaccagtccc atcaccgcga ggacgcgcgc gcgaagtgtt tgcggcaggt ggcgcgagaa
     5641 gatcgtcacc gcgcagcccc agccggcgag catcagcgac cacagcagca gcgagccctc
     5701 gtgcgagccc caggttgccg cgaaccggta atgcgcaggg agatgcgagt tcgagtgctc
     5761 ggcgacattt gcaaccgaaa aatcgctcgt cacgaaggcg taggcgagac agccgaacgc
     5821 gagtgccacc agagcgaact gcgcgcgcgc ggcgggcacg gcgacgctca tcagcgtggc
     5881 gtcgcgccgg gccgcgccga gcatcggcat gacgccctgc acgatcgcga cgagcagggc
     5941 gaggatcagt gcgaaatggc cgatctcggg aatcatggtt gctcgcgttg tctggggaaa
     6001 tcggggtcag agctgtaatt cgcgacgcac ggttgagcgg gcgcgagcag ttgtacgcga
     6061 attacagctc tgaccccgat taagggaatc atcgttgggg ctcgctcatg ctcgacatcg
     6121 tcatcgctct tttccggtgc gcctggtcga tcgcatcctt cgccgcgggc ggcatgtagt
     6181 tctcgtcgtg cttcgcgagc acctcggtgg catggaacgc gccatcgctg ccgagcgtcc
     6241 cttgcgccac gacgcccttg ccttccttga acaggtcggg cagcaggccc gtgtacgtca
     6301 ctggaatcgt ctgcgcggta tcggtgacgc gaaagtgcac cgtcagcgaa ttcggatcgc
     6361 gcgtgaggct tccttgctcg accagtccgc cgatgcgaaa cggccgcgat tgcggcgctt
     6421 ccttcgccac cacctgcgat ggcgtgaaga agaacacgag gttcgactgg aacgcgttca
     6481 gcaccagcgc gacagcgacg ccgagcgcca cgagtccggc gccgatccac gcgaaccggc
     6541 ggtggcgcgg cttcatcgcg ggctgccttt gcgtgtcatc gttgcacccg cgggctgcgc
     6601 atcgaccgac atccgcaatt cgtcgaacgc gcgcttgcgc cgtgcccgca ccgcggcgat
     6661 ctcgacggcg atggcgagcg ccgcgacccc gtacgccatc cacacgtacc acgcgtagcc
     6721 gcccatcgcg aagaaagcgg ccatcaggcg ttttctccgg cggggaaccg gatcagcgac
     6781 tcgagccatc ccgcgccgcg ctcgcgctcg acgatgatcg cctgcacccg cgcgagcacg
     6841 acggcaatcg tgtacgacca tgcggcggcg gtcatcacga gaatgccggc gagcatcgtc
     6901 atcgccatct tcggtgccgc cgtcaggctg atcgacgagc cctggtgcag cgtgttccac
     6961 cattgcacgg agaaatagat gatcggcacg ttgaccgcgc cgacgagcgc cagcacggcg
     7021 caggcgcgat cggcgcgccg gggatcctcg agcgccgcgc gaagcgcgat gatgccgagg
     7081 tagaggaaaa gcaggatcag cgtcgacgtc atgcgcgcgt cccacgccca gtaggttccc
     7141 catgtcgggc ggccccagaa ggcgccggtc cacagcgcga gcgcggccat cagtgcgccg
     7201 gtgggcgcca gcgcctgcgc catcatcgcc gacagccgcg tgttgaagcc gagcgccagc
     7261 gcgctccagc ccgccatgac gagataggtg aacatggcca tccacgccgc gggaacgtga
     7321 atgaagatga tgcggtaggc gtcgccctgc gtcgcatcgg tgggcgcgat ccatagcgcg
     7381 atcacgatcc cggcgcccgc cagcaacgcc gcgagcgcgc cgaacgcata cgaccagctt
     7441 cccgcgatcg ggtagaacga aatcggcgac gcgaagcgaa accagttgat caaaatagac
     7501 aatcggcaat tcaatcagtg attttaccgc acacatcccg ttcgctcgct ggcgccggcg
     7561 gcctcgccgt atgcggtttc cccgtccccg ccttgctgct gtcagctctg cacatgtccg
     7621 cctcgagccc gaaattgcgt atttgattaa cagggagcgc gcgagcgctc ttcaggcgcc
     7681 gggctcgccc atttcgccgg actcgacgcc acatgtcgct tcagcgaggt aggagaacat
     7741 cctgcgccgc cgaagccggg tcgtgcatga acgagtaggg gagcgatcat gaggacgcga
     7801 attgccggtg caactggact ggtccggtct gtggttgtac tgatgctggt tctggcggtg
     7861 ttgttcccct cggccgcgca ggcgggcacc gacgttcaga cgggcctcgg tggcaatgag
     7921 ccgaccacgg ccgcggtgaa cccgcgcagc ccgagtgtga tcgcggttgc acgcgggttg
     7981 tctgtcgcca tctccaccga tttcgggcgc acgttcccga cggtcgcgaa catgccgcag
     8041 ggcggcggcg gcgtccccgc aacctacggc acgattgcca cctggaattt ctgcggcgat
     8101 ggttccgtca ccttcgattc ccagggccgt ctgttcatga cgtacctgct gtgcggcaac
     8161 gacaacacgg tgcctacgcc gaaccgcgtc gacgtttcgt cgttcgtcca gcaggtcaac
     8221 cccaccacgg gcgcgctgat cggcaatccg gtcgaggtca cgggggcgaa cgcgctcaac
     8281 aatgacgaca aggaatggat cgcggcggac gccaaccccg acagtccctt ccgcgacaac
     8341 ctgtacgtcg tctggtcgcg gctcaacacg tcccagatca tgttctcgcg ctcgaccgac
     8401 ggcggcctca actggtcggc gcccacggcg atcagtgcgg ctggcgaagg cttcgtctgg
     8461 ccgagccacg tcgcagtcgc gcccaatggc gacgtttacg tcagctacca cggggacacc
     8521 tgcggcgcgg ccacggcgca gatgttcgtg ctgcgcgata gcaacggcgg tgggcaattc
     8581 caggcgggga ccggtttcca gaagacgtcg ttcgcgtcgg ccgtttcctg taaccgacgc
     8641 gatgcttcgg ccagcattcc gaacgcccag ttctggatgc agggggcgaa tcagggtttc
     8701 gtcatccccg atccgatccg gccgggcaac gtatacgtcg tcgtcaacga cgacccgcag
     8761 gacaatttca cggcaaccac cggcgaccat gccgatgtaa agcttgcgcg ctcgaccgac
     8821 aacggcaaca catggacgct cagtaccatc agtcacgcac cccccgggac ccttcaggtc
     8881 tttccgacgg gcgcgagcga ccagctcggc aatctcgtca ttacctggtg ggatacgcgg
     8941 cgcggcttca ccaacgccgc aggcaacctg ctgctcgaag tctatgcaac ggtcagccgt
     9001 gacggcggtg tcacgttcac gaacgacttt cgcgtcagcg atcttccatt cgatcccgac
     9061 ctcaacgcct cgtgtcgatt cggcccggcg gcgacctgcg gcacggtcac cggcgccggt
     9121 accgccagca ctttgcgcat cggtgaatac aatggcgtcg ccgccgccga cggaaccggg
     9181 tatgtgatct ggagcggcaa ccagacgccg cccaatccgc aggccgtccc gccgaccgcg
     9241 gccgccggcg gccagaccac ctacttcgac gtcttcacca tcctcggcgc gttccccgac
     9301 gcggcggagc ccaacgacgc gtgggatccc ggcgtggcct cggtgctcgg cgcgaaccgc
     9361 acgtaccaga accaggatct gacaatccat tcgagcaccg atgaggactg gttcaaggtc
     9421 accgcgctcg ccacggggca gctcttcttc acgatcgact acaacagccg cttcgccgac
     9481 ctcgatttgc aggtgcgcga caagttcaac aatccggtcg ccacgcagac tctggggctc
     9541 gacgtgaaca atacggagtc gatcttcgcc ccggcggtcg ccggcgaggc gtacttcgtg
     9601 cgcgtgttcc ccgagcccgg gcaggcaccg tcgctcaaca cctacgacct cggcatcgtc
     9661 aataccgcgg cgcccgttcc attcggcatc gtgcttgccc cagggtctga cagtggctcg
     9721 agcgggagcg acaacatcac gaagatcgcg acaccgacga ttcgcctcgc cgtcgatctc
     9781 gacccgctcg cgccattggc cttttcaccc gatgacgcca cgccgacgct gaccgacgat
     9841 gcgccgggct acaaggtcga ggtgtaccgc aacggcgtca ccgccggacg cgcgacaccc
     9901 gtcgccgggc agccgggcat cttcgaattc acgttcacgt caccgcttgc ggaaggccag
     9961 aacttcctca cggcgcgggt cgtgatcgtc gacaattcgg acaacgccgc cgtcgccggc
    10021 acgcttcacg ccgttggttt cggcggcgaa tcgcagaccg tcgtcgtgac gctcgacacc
    10081 acggcgccaa ccggcgcgtc gctcggcccg ctcgatctgc tcgcgtcgag cgattctggc
    10141 gggatcagcg acgacaacat cacgacgttc agcacgccgt cgttcggcct tcaggtcaac
    10201 gagcccgggt tcatccgcat ctacgctgca accgttcccg gtggaatccc gatccaggtc
    10261 gggcagatcc aggcgacgac tgcaggggcg tggcaggcga cggttcagtc gctcgccgac
    10321 ggggtctacg acatgacggc gacgatcgaa gacgctgccg gcaacgtcgg gcctccgacc
    10381 tcggcgctgc gcgtgacgat cgcgaagaat tcgctgacgc tgcccgggta taccgtgggc
    10441 ggggcggggg gtccagtgac gatcgatctc gcggcgagaa ccatcagcgg ctttgcgagt
    10501 gcgtccccga gcctgctgat cggcatcgct ggcattccgt cggtcaacgt cgacatgaac
    10561 ggcatgacac taaccgtcaa tctcacgaac ggcaacgacg cgttgagcta caccccgtcc
    10621 gggccaggat cgggcagcat cgcgctcgcc ggcgtgaacc aggtgctgaa cttcactggt
    10681 tccggcatgt tcacggtcaa tccgctcggt ggaaacgata cggtcacgac gtacggatcc
    10741 tcggtgggtg acgccgtgaa ggtcgaggtc gacacgaccg tcgccgtgca ggtggggagc
    10801 gcgctgacgc tcaacatgcc cgcgtcgcag atcgagaagg taggcgtttc cactttgcag
    10861 gggaacgata cgatcaacgt caacattcat gacaccgtca atgcgtcgct gttcgtcgac
    10921 ggtggcgatc cgaccaccgt caacaagggc aacgacgtgc tgaacctcat cgacgcatcc
    10981 gcgacgaaga agggccagta ctcgaacatc tccggcggca gcacatccgg ttcgggtgcg
    11041 gtggtgttga agttcaaatc tgccggcacc gcgacacgca tcgactacgt caatatcgaa
    11101 aagcagacac gcaagtagac gcgcggcgca gggcttccag cccttcgccg taagaacgac
    11161 ccccggcggg cgcagtctcg ccgggggttt ttttgccggt acaatccacg gccctgcctt
    11221 cgcaggatat tgcgccttcc gtcatgagcg acgccgcaac gagcgcacca gcgacgatca
    11281 cgctgctgat cgaccgcgcc aactcgggca atgcagaggc gctgaacgcc cttttcgcga
    11341 cgctctatcc cgagttgcat gcgctcgcgc acgcgcgcat tcgcagaaac cgccagctca
    11401 cgctgctcga tacgacggct cttctccacg agtcgtatct acgtttcgtg aggctcgggc
    11461 agctcaacgt cgggaatcgc gcgcactttc tcgcctatgc cgcacgcacg atgcgttcga
    11521 tcgtcgtcga ctttgcgcgc gcgcggctcg cgcagcggcg cggcggcgga ggtgacgatg
    11581 cgatcctcga taccgatgcc gcattggcgg gcaccggaga ggaagaggtg atcagggtcc
    11641 acgaggcgct cgacgaattg cgccatgcgg acgaacggct cgtgcgcgtc gtcgagatgc
    11701 gctatttcgg ggggctgacc gaagacgaaa taggcagtgc gctgggaatc accgaccgta
    11761 cggtccgaag gatgtgggac aaggcgcgcg tgatgctcga agtcgccctc cgatagccgg
    11821 gtatctcgcg cacgtcgatt ccgccgcttc catgaagctc gaacccgcgc aatggcgggc
    11881 actctccgtg ctgctcgacg aggcgctcga actcgagccg accgggcgcc gcgcgtggct
    11941 cgacgcgttg tcgggagaag cacgcggatt ccagccgatg ctcgccgatt tgctcgtgcg
    12001 aacggaagca ggggcagaaa tgttcctgga cacgctgccg cgtatcgctg caccggatcg
    12061 caccccggca ttcttcgccg gcggccgcgt cggcccctat cggttgctgc ggcgactcgg
    12121 tcgaggagga atgggtgagg tctggctctc cgagcgcgcg gacggcgtac tcaagcgccc
    12181 ggttgcgctg aagcttcctt tgctcgcgac gaacccggac atccttgccg aacgcttcgc
    12241 gcgcgaacgc gagatattgg catccctcgc acacgcgcac atcgcgcgtc tctacgacgc
    12301 gggcttcgac atcgaagggc aaccctacct tgcgctcgag tacatcgacg gcataccgct
    12361 gacgcattac gccgacgtgc atcggctcgg cgttctcgcg cgtcttcgac tgttcgacca
    12421 ggtgctagac gccgtcgcgc atgcgcatgc gagcctcgtg ctccatcgcg atctcaagcc
    12481 gtcgaacatt ctcgtgacac ccgccggcga cgtgaagctg ctcgatttcg gcatcgcgaa
    12541 gttgctgcaa tccggcgccg ccgaagactc ggcgctcacg cgaatttcgg gtcaggcgct
    12601 cacgccggac tacgcatcgc ccgagcagat cacaggcggg ccgttgacga ccgcggcgga
    12661 tgtctacggg ctcggcgtcg tgttgtacga gctgctgacc gggaagcgtc cctatcggct
    12721 gaagcgcgga tcgcccgccg agctcgagga ggcgatcgcc ttcgtcgacc ccgcggcccc
    12781 gtctcgggcg cgcatcgtcg acgcggcagc ggcgcagcgc gcgacgaccg tccaccggct
    12841 ccggcgcacc ctctcgggcg atctcgacac gatcgtgctg aaggcgctgc gcaagcggcc
    12901 cgacgagcgt tatgcgggtg cgcgcgcgct cgcggaagac ctcgagcgct accgcgacgg
    12961 tgcacccgtc ctggctcggc ccacgagcgt ttcgtatcgg accgcgaaat ggatgaagcg
    13021 acaccgtgcg ggggttgcgc tcggaagcgt ggttgcggca acgctgctcg gcgccaccgg
    13081 tatcgcattg catcaagcgc gcgtggcgag catcgaggcg cagcgcgccg aggcaagcaa
    13141 cgattttctg atgggcctct ttcagcatgc cgcacgcaac aatccgggtg gcgccgcggc
    13201 cgccgacacg acccttcggc agttgctcgc catcggcagc aggaaagtgc tcgacgattc
    13261 gcgcgcgagc cccgagttgc agctcgacgt atcggcgctg ctcgcgcggc tcaacctcga
    13321 ggtcgatctc gtcgatacgg cggagagact gaccgagcga tcgctcgtgc ttgcccgcaa
    13381 attgcacggc gagaacagct tgcccgtcgc gcaggtgctg gcgcagcacg ccgacaacct
    13441 ctatcggtcc ggccgctacg ccgaagcgat cgaggcgtcg cgccaggtgc tcgcgcacgt
    13501 cgatcgggcg cgcgcaccgg cagacgagct tcgtgcgcga gcacacatcc ttatcggcaa
    13561 ttcgatgttc caggtcgatg cgtcgaaaac cgccgagccg ttgcagcatc tcgagacggc
    13621 cctcgaattg ctgaagcgcg cccatgcgac gaccgaggat cgctcgcgcg ccgcgtactt
    13681 catcggatgg attcatgaaa gcaccggcga tttcgcgcgg gccgagacgt attacaacga
    13741 tggcatcgag gccggccgcg cgaacttcgg ggagcgcagc ttcatcgtcg cattcggcta
    13801 tgagggcttg gcagaatcgt tgcgccggca gcgacggctc gccgaagcgc gcgatacgat
    13861 gggccgcgca atcgacatct accaatacgt gcttgcgcct cggcatgcga cggtcgcgtt
    13921 cgcgcggacg agcctttcga tgatcgaggc gtcgtcgggc cgcctggccg aggcggagcg
    13981 gctggcggac gatgccctcg ccgtcacgaa gacggtgttc ggcgagcacg cgcggcagac
    14041 ggtcgttccg gcgacgcaag cggcgcgcct caaggtccag cgcggcgagt tggaatccgg
    14101 gttggcgctc tacgaggacg cgctcgcagc gctcgtcgcg gaagaccggt cctccctcac
    14161 caatcgcgca cttcgcatcg agtacgcgga agccttgctg gaaacgggtg agatcggaaa
    14221 ggcgaggacg gcgctcgacg aagcgcaggc gggattcgag gcggcgcacg acctcgcaag
    14281 cgtgcgcggc gcatggttgc gcctcgtccg cggcgaactg gcggttgctg ccggcgactc
    14341 cgacgcggca tcggccttcg atcacgtgct cgactctttc acgcggtccg accagggcgc
    14401 acccgcactg gcgcgtcgcg ccgcgaacgc gactgcacgg tattcgccga cacccgagcg
    14461 cgcggagacg atgctgcaaa agctccccga gttcggcctg ctgccgactt cgccggacga
    14521 gctcgatatc gacatcgtcg acaaggcgca gctcgccgat tcgatcggtc ggctcttcaa
    14581 cgtagcggga aagcgtccgg aggcgttcgc atggctttcg aaggccgtgg cgttgcgcga
    14641 gcaactggag gtgcccgaaa gcccacggct tcgcgatgcc cgtcttgccc tgacgcgagc
    14701 aggttccgtt aacgaacgcc gttgagcgag gccgcgaaaa cgcgcgtcgc tttcagtgtg
    14761 ccggtcggtg agctcaatcg agcgctatac gcacggctgc cgcaaccgcg aacggcacgg
    14821 cgacgagggc cagcagcaat cccgcgccga gcaacgacag ctgcggtgcg gccgaaacgc
    14881 cggcgcgtac agcatccacc gcgccggcgc caaagatcag caccggcgca tacagcggca
    14941 acacgagcag cgcgagcagc gcgccgccgc cgcgcgcgcc aagcgtcaat gcggcgccga
    15001 cggccccgag cagcgacagg atcggcgtgc caaggagcag cgacgccgcg agcgtggcga
    15061 gcaccggcgc atcgagcgcg tactgcgcgc cgagcagcgg cgccatcagt gccaccggca
    15121 atccgctggt aagccaatgt gcggccattt taccggacac cagtgcggcc agcggccgcg
    15181 gcgacagcgc gatctgctcg agcgtgccgt cgtggtagtc ggacgcgaag agccgcggca
    15241 gcgacaaaag cgacgcgagc agcgcgctcg tccacagcac gcccggcccc agcgtggcga
    15301 gggtttcgcg ggacggtgac gtggccaagg gaaagagcga gacgacgatg acgtagaaca
    15361 ggagctggac gcccagttcc cccttggacc gcagcgcgac gcgaaagtcg cgcgcgagcg
    15421 cccagccgac cgcggcgagc accgatgcgt gcgcgtcggc ggagctctcc gcaggcgcgg
    15481 gttgcacggc aagcgtctcg gcgagcggcg acggcttcat cacgctcacg ggcctactct
    15541 ccgcgcttcg cgctgcgagg ctgattcgcg cttgggacgg cccggcgcgc tcatgtcggc
    15601 gcaccgagct cgagacggcg cacgcggtcc ggcgcgatcg gcagcggctg atgcgacgcg
    15661 gcgatgcaca ggccgccttg cgccagatgc gcgtcgagca gcgcgccgag catgcccgcg
    15721 ccgtcggcat cgagcgcggt caacggctcg tcgaggatcc acagcgcgcg ccgcacgagc
    15781 gcaagacgcg cgaggccgac gcggcgccgc tgcccctgcg acagcacgcg caccggcaag
    15841 cgccgctgct tcagcaatgc gacggcatcg agcgccccgg cgatcgattc gcgcgatgca
    15901 cgcggcgcgt cgagcgcaat ccacgcgtcg agattttcct cggccgtcag ctcgtctttc
    15961 agggccgacg catggccgtt gaatgcaacg gcggcgcgaa gccgcggatc gaacgccgcc
    16021 acccgttcgc cgcgccagcg aagctcgcct tccgccggtg cggtgagtcc cgcgacgatg
    16081 cgcaagagcg tggtcttgcc gctgccgttt cgtccgctga cgacgagcgc ttcgcctgcc
    16141 tgcagcgaaa agtcgacgcc gtcgaaaagc cgcgcgtagc cgcgctgcgc cgccagccgg
    16201 cgtgcctcga tcgagttttg cgtggacatc cgaatgcgct tcagcgcacg acgtcgtcga
    16261 tcacgatcga cacatcctgc gcgcccggct tcacgacggt gctgttgccc tggagatcgc
    16321 cgggcgacgg cgtcgcgctc cccgtcttcg agatgcgcgc ctcgacgacg acatcgcttg
    16381 cacccgacaa gcgtgacgcc ggcgccatgg ccatcgaatc gtcgagcgtg aagttgcgcg
    16441 gcagctcgcc cgccgtggcg cgcagcacgg caagcggcat gcgcgggccg ttcactgcgc
    16501 gcgcgaaaat gaacagcgtg tcgttcgcag atgcgcgccc gcgcaatttc ggatcgagcg
    16561 acacgcgccc ggtgatcgcc gtatcgccgg cgttcgcggt ggtcgctgcg cctgccgccg
    16621 cgtcgttgcc gccacccttc gcgccgggcg acatcgcacc gcccttcccg ccgggcgaca
    16681 tcgcgccgcc ctgtctcgcc gcattggcct cggcgatcat cgaatcgatc tgcttcgctt
    16741 cgtcgctgcc cggcgcgaat tgcgccttca gcttgcgcca ctcgtcgatg gccacgccgt
    16801 aatccttgcg ctcgagcgcc gcggtcgcgg cgagcgacag cgccttcggt tgccgcgggt
    16861 cgatcgcgag cgctcgctcg atgagctgcg acggctcgcc ctgcagcgac tggttgtgca
    16921 tggcgagcgc gtcggcccaa tcggcgagaa gcgttgcatc tgccgggccg cgttcggccg
    16981 cttccttgaa cgcggcaacc gcttcgggaa agcggccgag tgctgcatac gcgcgcgcaa
    17041 gaagccgcca cccggtcggg tcctcgggat gttcct
//
LOCUS       DASSVX010000295         7305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1429875, whole genome shotgun sequence.
ACCESSION   DASSVX010000295 DASSVX010000000
VERSION     DASSVX010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429875"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VFO53_12745"
     CDS             complement(<1..158)
                     /locus_tag="VFO53_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET8877191.1"
                     /translation="MLALCSQALASDAQDTIARVKRSVVAVGTYERTRSPQFQFRGSG
                     FAVGTGAT"
     gene            complement(209..1168)
                     /locus_tag="VFO53_12750"
     CDS             complement(209..1168)
                     /locus_tag="VFO53_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET8877192.1"
                     /translation="MAARERALCTFVAFALASGASHAVFAAASAPPASPASASPNSTA
                     SISEKLARAMSYEHGEGVPKDPGIAAALYCEAAVQGSAEAAFQLGWMYANGRGIAHDD
                     GVAAALFELAASDGHAFAQTALAHLGNVRGLLPDCMRPLRPPPIADASLMADADPGPD
                     PFADLSPEKQKIRRIVDEIAPKYGIEPRLALAVIAAESNFNVAARSVKDARGLMQLIP
                     GTASRFNVRNAYNVRDNLKGGLSYLRWLLAYYQGQVALAAAAYNAGEQTVDRYGGVPP
                     YPETRAYVRRVLGLFRRPVHPYDPSVTEPSAIAATEHVGMQRD"
     gene            complement(1193..2272)
                     /locus_tag="VFO53_12755"
     CDS             complement(1193..2272)
                     /locus_tag="VFO53_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8877193.1"
                     /translation="MTPVDSAFHRARERLRSHPRRWLVTGSAGFIGSHLVEALLELGQ
                     QVVGVDNFATGHRRNLDDVHAASGEAAWSRHRFIEADIADLDACRRACKDIDVVLHQA
                     ALGSVPRSISDPLPAHAANVTGFLNLLLAARDAGVARFVYASSSSVYGDHPRLPKVED
                     VIGNLLSPYAVTKYIDELYAAVFARCYGMATIGLRYFNVFGPRQDPEGAYAAVIPRFV
                     AAMLRGTPVTIFGDGETSRDFCYVANVVQANVLAATVDDPDALNGVYNVAVGGRLTLN
                     ELHAALADLIARRHPALDIPPPRHADFRAGDVRHSEADIDKARRLLGYQAEWDVKSGL
                     AIALPWYESLLRPSAPETVGMRRVV"
     gene            complement(2283..3572)
                     /locus_tag="VFO53_12760"
     CDS             complement(2283..3572)
                     /locus_tag="VFO53_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET8877194.1"
                     /translation="MTDSTTIAVVGLGYVGLPLAVEFGKRYPTIGFDLSKAKIDAYRR
                     RVDPTGEVSARDFAAAERLEVTTDAAQLKRANFIVVAVPTPVDEAHQPDFSPLLGASE
                     AVGKHLARGSIVVFESTVYPGATEEICVPVLERHSGMRWQRDFFVGYSPERINPGDRE
                     HTLTKITKIVAGDTPQTLERVAEVYSSIVAAGVHRASSIKVAEAAKVIENTQRDLNIA
                     LMNELALICDRLGIRTRDVLDAARTKWNFLPFRPGLVGGHCIGVDPYYLTHKADKLGY
                     HPQVILAGRRINDGMGKYVAEQTVKQMIQAGFPVKSAKINVLGLTFKENCPDLRNSRV
                     IDVIHELRSYGADVYVHDPVADAGEAMHEYGVTLTTWDGLPRAHAVVMAVAHREFGAR
                     PVTDYVGKLEPRGLFVDVKCQSDAASLRAQGVSVWRL"
     gene            complement(3637..6414)
                     /gene="prsT"
                     /locus_tag="VFO53_12765"
     CDS             complement(3637..6414)
                     /gene="prsT"
                     /locus_tag="VFO53_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HET8877195.1"
                     /translation="MKILRIEYAGSVAAVLAALALAGCFGKDASSYVTSARGYLAKSD
                     YKAAIIEAKNALQKEPDNAEARVLLATSLLESGDPAGGEAETRKAIALHAPDDRTYPL
                     LARALAAQGQFSKIASEVGDRKFNDPAARSQVNVALAGAALAQGDPTKAKDLADAALA
                     DVPTSIPALLLEAQLAGSRGDLAAARKFADTALKSSPGDVGALMLKADIALAETKVDD
                     ARALFEQAIAAHPDSIAARTALLSLELRLGKLDKAKEQLAKMKEVGPRDIRTVYADAL
                     LASVSGDNAHARDAIQRVLAAKPDHLPSLLLSGMINYHLGSYGAAEQSLRSVIVKVPD
                     EPNARRVLALIYLRTGRAAQALEVLGPAMRRFPDNAILLRVAGEAYLASGNAEMAATS
                     YEHANAVDKNNVGSQVRLAQVRLAAGDTDRALTDLESLSQSDTKDAQADLALFSEHMR
                     RRQFDDAMADVDELEKKRPKTALVSNLRGSVFLAKRDLVNARANFERALAIDPRSFAA
                     ARNLALIDLQQGNSQAARARYEQMLSKEPGDEALLLAYADLLSVTAASPDKAREAIDK
                     AVAAHPGSVRARLALVGYDMRRRDAKAAMATAQAALSAIPNDPQLTEALGATQLASGE
                     FNQAVETFRNLVKLQPENPLALLRLAEAQVAIKDFPSAIDSERKALAMKPDLPAALVA
                     LTKTYVLAGKADAAIAEARRLQKEHPDKAMGYALEGELQALQKKWPEAASAFGAASSR
                     QPSALLVTRQYGALRGAGKAAEGTAIANKWMKEHPDDATIPLMLAEESQRRKDFPAAI
                     AGYEKVIALDPRNVVALNNLAWVLTEKKDAKGLEYAERAQQVTPFNPSILDTYGWALT
                     RNGDAKRGAEMLRMASRLAPGQAEIQLHLAQALAASGDKAGARQAVDGLTKLDKASPI
                     RIEAEKLQATL"
     gene            complement(6442..>7305)
                     /locus_tag="VFO53_12770"
     CDS             complement(6442..>7305)
                     /locus_tag="VFO53_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET8877196.1"
                     /translation="KVAPTSATVLILGESGTGKELLARAVHELSPRRGERFVAINCAA
                     IPETLLESELFGYEKGSFTGAVKQTLGKIETANSGTLFLDEIGDLPVALQVKLLRFLQ
                     QRVIERIGGRQEIPVDVRIVCATHQSLKAQIEQGKFREDLYYRLAEIIVEVPPLRARS
                     GDAALLAHGFVRRMAEEQRRGPMSLQADALEAIEAHDWPGNVRELENVIKRAVIMTDG
                     SSISPADIGFETANGNADALNLRHVREQAERNAVLRVLGRVNGNLSKAADLLGVSRPT
                     LYDLMHRFGLK"
BASE COUNT         1194 a         2431 c         2412 g         1268 t
ORIGIN      
        1 atcgtcgcac ccgtgccgac ggcaaagccg ctccccctga actggaattg gggactgcgc
       61 gtacgctcat aggtgccgac ggcgaccacc gagcgcttga cgcgcgcgat ggtgtcctgg
      121 gcgtccgacg ccaacgcctg cgagcacagc gccaacatcg tgcagagcgc ccatgcgcgg
      181 atcagcgacg acacggcggg tgcagacttc aatcgcgctg catgcccacg tgttcggtgg
      241 cggcgatcgc cgaaggctcg gtgacgctcg gatcgtacgg atggaccggc cgcctgaaaa
      301 gaccgagcac gcggcgcacg tacgcgcgcg tctcgggata gggaggcacg cccccgtacc
      361 ggtcgaccgt ctgctcgccc gcgttgtacg cggcggcggc gagcgccacc tgtccctggt
      421 aataggccag cagccagcgc aggtacgaca ggccgccctt gaggttgtcg cgcacgttgt
      481 acgcgttgcg cacgttgaac cgcgacgccg tcccgggaat gagctgcatc agcccgcgcg
      541 catccttcac cgaacgcgcc gccacgttga aattggactc ggcggcgatg acggcgagtg
      601 ccaggcgcgg ctcgatgccg tacttcggcg cgatctcgtc gacgatgcgc cggatcttct
      661 gcttctcggg cgacaggtcg gcaaacggat cggggccggg atcggcgtcc gccatcaacg
      721 acgcatcggc gatcggcggc ggccgcagcg gtcgcatgca atcggggagg aggccgcgga
      781 cgttaccgag gtgcgcaagt gcggtttgcg caaacgcatg cccatcgctc gccgccaatt
      841 caaacagcgc ggcggcgacg ccatcatcgt gggcaatgcc gcggccgttc gcatacatcc
      901 agccgagctg gaacgccgct tcggcgctgc cctggaccgc ggcctcgcaa tagagtgccg
      961 cagcaatccc ggggtccttc ggcacgcctt cgccgtgctc gtacgacatt gcgcgtgcga
     1021 gcttctcgga gatcgacgcg gttgagttcg gcgaggccga cgccggcgac gccggcggcg
     1081 ccgaagcggc ggcgaagacg gcatgcgatg caccgcttgc caatgcaaat gcgacgaacg
     1141 tgcagagcgc gcgctcgcgt gcagccatgc cgggctcgcc tcgacgcatg tcttagacca
     1201 cccgccgcat tcccacggtc tcgggagcgg acgggcgcaa aagactttcg taccagggca
     1261 gcgcgatggc cagaccgctt ttcacgtccc attccgcctg atagccgagc aaccggcgcg
     1321 ccttgtcgat gtcggcttcc gaatgacgca cgtcccccgc gcgaaaatcg gcatgacgtg
     1381 gcgggggaat gtcgagcgcc ggatgccgcc tggcgatcag atcggcaaga gcggcgtgaa
     1441 gttcattcag cgtgagccgc ccgccgaccg ccacgttgta gacgccgttc aatgcatcgg
     1501 ggtcatcgac ggtggcagcg agcacattgg cctgcacgac gttcgccaca tagcagaagt
     1561 cgcggctcgt ctcgccatcg ccgaaaatgg tgaccggcgt tccgcgcagc atcgcggcga
     1621 cgaagcgcgg gatcaccgcc gcgtacgcgc cctcgggatc ctgccgcgga ccgaacacgt
     1681 tgaagtagcg cagcccgatc gttgccatgc cgtagcagcg ggcgaaaacc gcggcataga
     1741 gctcgtcgat gtacttcgtc accgcgtagg gcgagaggag atttccaatg acgtcctcga
     1801 ccttcggcag gcgcggatgg tcgccataca cggagctcga cgacgcatag acgaagcgcg
     1861 ccacgcccgc atcgcgcgcg gcgagcagca gattcaggaa accggtgacg ttcgcggcgt
     1921 gcgcaggaag cgggtcggag atcgaccgcg ggaccgagcc cagtgcggcc tgatgcagca
     1981 cgacgtcgat gtccttgcat gcgcgccggc acgcatcgag atcggcgatg tcggcctcga
     2041 tgaagcgatg ccgcgaccac gcggcctctc cgcttgccgc atgcacgtcg tcgagattgc
     2101 ggcgatggcc ggttgcgaaa ttgtcgacgc cgaccacctg ctggccgagt tcgaggagcg
     2161 cttcgaccag gtgcgacccg atgaacccgg cgctgccggt gacgagccag cgtcgcgggt
     2221 gcgagcgcag ccgctcgcgc gcccggtgaa atgcgctgtc gaccggcgtc atggggcgaa
     2281 cgtcacagcc gccagaccga aacgccctgc gcacgaagcg acgccgcgtc ggactggcat
     2341 ttgacatcga cgaacaagcc gcgcggctcg agcttcccga cgtaatccgt taccggtcgc
     2401 gcgccgaact cacggtgtgc aacggccatg accactgcgt gtgcgcgcgg caggccgtcc
     2461 cacgtcgtga gcgtcacgcc gtattcgtgc atcgcctcgc cggcgtcggc caccggatcg
     2521 tgcacgtaca cgtcggcacc gtacgagcgc agctcgtgga tgacgtcgat gacgcgcgag
     2581 ttgcgaaggt cggggcagtt ctccttgaac gtgagaccga gcacgttgat cttcgccgat
     2641 ttgaccggga acccggcctg gatcatctgc ttgaccgttt gctcggcgac gtacttgccc
     2701 atgccgtcgt tgatgcggcg cccggcgagg atcacctgcg ggtgatagcc gagcttgtcg
     2761 gccttgtgcg tgaggtaata gggatcgacg ccgatgcagt gcccgccgac gaggcccggc
     2821 cggaacggca ggaagttcca cttggtcctg gccgcatcga ggacatcccg cgtgcggatg
     2881 cccagccgat cgcagatcag cgccagctcg ttcatcagcg cgatgttgag atcgcgctgc
     2941 gtgttctcga tgaccttcgc cgcttccgcg accttgatcg aagacgcccg gtgcacaccg
     3001 gccgcgacga tgctggaata gacctccgcc acgcgctcga gcgtctgcgg cgtgtcaccg
     3061 gcgacgatct tcgtgatctt cgtcagcgtg tgctcgcgat cgccgggatt gatgcgctcg
     3121 ggcgaatacc cgacgaaaaa atcccgctgc cagcgcattc ccgaatgccg ctcgagaacc
     3181 gggacgcaga tctcttcggt ggcgccggga tacacggtgg attcgaacac gacgatgctg
     3241 ccgcgcgcca gatgcttccc caccgcttcg cttgcgccga gcaacgggct gaagtcgggc
     3301 tggtgcgcct cgtccaccgg cgtcggtacg gcgacgacga tgaagttggc gcgcttcaac
     3361 tgcgccgcat cggtcgtgac ctcgagacgt tcggcggcgg cgaagtcgcg cgcgcttacc
     3421 tcgccggtgg gatcgacacg gcgccgatac gcatcgatct tcgcctttga aagatcgaag
     3481 ccgatcgtcg gataacgctt gccgaattcg acggcgagcg gcaggccgac atagccgagg
     3541 ccgacgacgg cgatggtggt cgaatcggtc atgtgcgagc ccaacgcctt cgtgcgagcg
     3601 cgtggctgaa ggaatcctgt tcgacgacgg gccccatcaa agcgtcgcct gcagcttctc
     3661 cgcctcgatg cggatcggcg acgccttgtc gagcttggtc agcccgtcga ccgcctggcg
     3721 ggcgcccgcc ttgtcgccgg acgcggcgag cgcttgcgca agatgcagct ggatctccgc
     3781 ctgtcccggc gcgagccgcg acgccatgcg caacatctcg gcgccgcgct tcgcatcgcc
     3841 gttgcgcgtc aacgcccatc cgtacgtgtc caggatgctg gggttgaacg gcgtaacttg
     3901 ctgcgcgcgt tcggcgtatt cgaggccctt cgcgtccttc ttctcggtca gtacccacgc
     3961 gaggttgttc aacgcaacca cgttgcgcgg atcgagcgcg atcaccttct cgtatcccgc
     4021 gatggcggcc gggaaatcct tgcgccgctg gctctcctcg gcaagcatca gcggaatcgt
     4081 ggcgtcgtcg ggatgttcct tcatccactt gttcgcgatc gccgtgcctt cggcggcctt
     4141 gcccgcgccg cgcaacgccc cgtattgccg ggtgacgagc agcgcgctcg gctgacgcga
     4201 cgaagcggcg ccgaatgcgc tcgccgcttc gggccatttt ttctgcagcg cctgcagttc
     4261 accctcgagc gcatagccca tggccttgtc ggggtgttcc ttttgcagcc ggcgtgcttc
     4321 ggcgatcgcg gcgtccgcct tgccggccaa aacgtaggtt ttggtaagcg ccaccagcgc
     4381 cgcgggcagg tcgggcttca tcgcgagcgc cttgcgctcg ctgtcgatgg ccgacggaaa
     4441 atccttgatc gccacctgcg cctcggccag gcgcaacagc gccagcggat tctcgggttg
     4501 caatttcacg agattgcgga aggtttcgac cgcctggttg aattcgccgg atgcgagctg
     4561 agttgcgccg agcgcctcgg tcagttgcgg gtcgttggga atcgccgaca acgcggcttg
     4621 agccgttgcc atcgccgcct tcgcatcgcg ccgacgcatg tcgtaaccga cgagcgcgag
     4681 acgcgcacgc accgaaccgg gatgcgcggc gaccgccttg tcaatcgctt cccgggcctt
     4741 gtcgggcgac gccgctgtca ccgaaagaag atcggcatac gccagcagca atgcttcgtc
     4801 ccctggctcc ttcgacagca tctgctcgta ccgggcacgt gccgcctgcg aatttccctg
     4861 ctggagatcg atgagcgcga ggttgcgcgc ggcggcgaaa gatcgcgggt cgatcgcgag
     4921 cgcgcgttcg aaattggcac gcgcattcac gaggtcccgc ttggcgagaa acacgctgcc
     4981 gcgcagattg gacaccagcg cggttttcgg ccgcttcttc tcgagctcgt cgacgtcagc
     5041 catcgcgtcg tcgaattgac ggcgacgcat gtgctcgctg aacagggcga ggtccgcctg
     5101 cgcgtccttg gtgtcgctct gggacaatga ttcgaggtcc gtgagtgcgc ggtcggtgtc
     5161 gcccgcggcg agcctgacct gcgcgagcct gacctggctg ccgacgttgt tcttgtcgac
     5221 cgcattcgcg tgctcgtacg acgtcgcggc catttcggca ttgccgctgg cgagatacgc
     5281 ttccccggcg acgcgaagca atatcgcatt atccggaaag cgccgcatcg ccggcccgag
     5341 cacctcgagc gcctgtgccg cgcgtccggt gcgcaggtag atcagcgcca gcacacgacg
     5401 cgcattgggc tcgtcgggca ccttgacgat cacgctgcgc aatgactgct cggccgctcc
     5461 gtacgatccg aggtggtaat tgatcattcc cgacagcagg agactgggca ggtgatcggg
     5521 cttggccgcg agcacgcgct ggatggcatc gcgggcgtgg gcattgtcgc cggacacaga
     5581 ggcgagaagc gcgtcggcat agacggtgcg gatatccctc ggacccacct ccttcatctt
     5641 cgcgagttgc tccttcgcct tgtccagctt gccgaggcgc agttccagcg aaagaagcgc
     5701 ggtacgcgcg gcgatcgaat ccggatgcgc ggcgatcgcc tgctcgaaca gcgcgcgtgc
     5761 gtcgtcgacc ttcgtttcgg cgagcgcgat gtcggccttc agcatcaaag cgccgacgtc
     5821 gcccggcgac gatttgagcg cggtatcggc aaatttgcgt gccgcagcca gatcaccccg
     5881 cgaaccggcg agttgcgcct cgagcagcag cgcggggatg ctcgtgggaa cgtcggcgag
     5941 tgctgcatcg gccaggtcct tcgccttggt cggatcgcct tgcgcgagcg cggcgccggc
     6001 aagcgccaca ttcacttgcg agcgtgcggc agggtcgttg aacttgcgat cgccgacttc
     6061 gctcgcgatc ttgctgaact gcccctgcgc cgcaagcgca cgtgcaagaa gcgggtaagt
     6121 acggtcatcg ggcgcatgca gcgcgatcgc cttgcgggtc tcggcttcgc cgcccgcggg
     6181 atcgcccgat tcgagcaacg acgtcgcgag cagcacgcgg gcctcggcgt tgtcgggctc
     6241 cttctggagc gcgttcttgg cctcgatgat cgccgccttg tagtcggact tggcgaggta
     6301 gcccctggcc gacgtcacgt acgacgaggc atccttgccg aaacagccag cgagcgccag
     6361 ggccgccagc acggctgcca cgctgcccgc gtattcaatt cgcaagattt tcatggtttc
     6421 cattcgaaaa ggccacggtc gtcatttgag gccgaagcgg tgcatgagat cgtacagcgt
     6481 ggggcgactg acccccaaca aatcggccgc cttgctgagg ttgccgttga cccgccccaa
     6541 tacgcgcagc acggcattgc gctcggcctg ctcgcgaacg tggcgcaggt tgagcgcatc
     6601 ggcgtttccg ttcgcggtct cgaagccgat atccgccgga gaaatgctgg agccgtccgt
     6661 catgatcacg gcgcgcttga tcacgttctc gagctcgcgc acgttgccgg gccagtcgtg
     6721 tgcctcgatc gcctcgagcg catcggcctg cagcgacatc ggtccacggc gctgctcttc
     6781 ggccatgcgc cggacgaatc cgtgcgcgag cagcgcggca tcgcccgacc gcgcgcgtag
     6841 cggcggcacc tcgacgatga tctccgccag ccggtaataa aggtcctcgc ggaacttgcc
     6901 ctgctcgatc tgcgccttga ggctttggtg cgtcgcgcac acgatgcgca cgtcgaccgg
     6961 gatctcctgc cggccgccga tgcgctcgat cacgcgctgc tgcaggaaac gcagcagctt
     7021 cacctgcagc gccacgggaa gatcgccgat ttcgtcgagg aacagcgtgc cgctgttggc
     7081 cgtttcgatc ttgccgagcg tttgcttgac tgcgcctgta aacgagcctt tttcgtagcc
     7141 gaacaactcg ctttcgagca gcgtctcggg aatcgcggcg caattgatgg cgacgaatcg
     7201 ctcgccccgg cgaggcgaca gctcgtgcac tgcgcgcgca agcagctcct tgccggttcc
     7261 cgattcgccg aggatgagca cggtggccga cgtcggcgcg acctt
//
LOCUS       DASSVX010000296        13448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_980046, whole genome shotgun sequence.
ACCESSION   DASSVX010000296 DASSVX010000000
VERSION     DASSVX010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 13448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13448
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980046"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1997
                     /gene="bamA"
                     /locus_tag="VFO53_12775"
     CDS             <1..1997
                     /gene="bamA"
                     /locus_tag="VFO53_12775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HET8877197.1"
                     /translation="TISSLSFSGNKEFDTDTIKKALKDIGIAEARIFDRSALDRAEQE
                     LKRQYITRGKYAANIQTTVTPQERNRVAINFTIDEGESAKIARINIVGTKAFTEKQLL
                     SEMALTTPGWFTWYTKNDQYSKQKLSADLEALRSFYQNRGYLEFNVDSTQVSITPDKE
                     DIYITLNITEGPRFNVGDVHIAGDLVVPESELAPLVQLKPGDVFSRAKLQASAKDISD
                     RLGAEGYAFANVNAVPELDRDNAKASFTFYVDPGRRAYVRKINITGNSKTRDEVIRRE
                     FRQIEDAWYDGPRIERSKVRVRRLNYFDNVSVETPPVPGSADQVDVEVSVTEKSTGNL
                     LAGVGYSSDEGIVLNASISQDNIFGTGNALSLGVNTGRFNRTYSMLFTQPYYTVDGVS
                     RTLEAYDKSLDPTGLAISQYASDTLGTAVGFGVPITETDTINFGGRYEHTRITLFDNS
                     PPVYQQFVKDFGTASNAYILTAGWSRDTRDDVLYPTRGRLQSVLVETGLPFGDLAYYK
                     VQYTHSVYWPLYGDLVLMARGDFGYGGGLSGKPLPFFKAFFAGGVNSVRGYDTASLGP
                     QDQFGNALGGRRKIVGNLEAFYPIIKGDKAVRASVFIDAGEIYANGQQSQFESFRYST
                     GVGVAWSSPIGPLKFSYAVPLNAREGDRQQRFQFQAGAAF"
     gene            2019..2549
                     /locus_tag="VFO53_12780"
     CDS             2019..2549
                     /locus_tag="VFO53_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET8877198.1"
                     /translation="MSIIDRQAVLARLRTAAVALALVAFAAAAAAADYKIGFVNTERL
                     FREATPAKRAQAKIEKEFATRDAEIQKLAKQVRDLQSALDRDGATMSDADRRNRERDL
                     ANQSRDLTRMQREFREDLNLRRNEELAGIQERANKVIQQIAADEKYDLILQDPVVYAS
                     QRIDITDRVIKALADK"
     gene            2594..3652
                     /gene="lpxD"
                     /locus_tag="VFO53_12785"
     CDS             2594..3652
                     /gene="lpxD"
                     /locus_tag="VFO53_12785"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HET8877199.1"
                     /translation="MASLRTLAGKPLSELAALTGAALDGDGSVVVTRVATLERAERGD
                     IAFLANPRYRGQLAATRASAVIVDPAAATATPLPKLVAANPYAVYARVAAILHPAPAP
                     APGAHSHACIDASATVAASASVGPYAVIGRHATIGERAAIGAHCIVGAGASIAADVVL
                     DARVTVYPGCVIGARTLVHSGAVIGADGFGLAEEDGRWLKITQIGHVVIGADVEIGAN
                     TTIDRGAIDDTIIEDGVKLDNQIQIGHNCVIGAHTAIAGCVGIAGSTRIGAHCRIGGA
                     AMISGHLEIAAGSAISAATLVYDSIRDPGIYTGAFPALPHREWKRVASQIRRLRELAE
                     RVSRLEAALTARQNDKNV"
     gene            3781..4248
                     /gene="fabZ"
                     /locus_tag="VFO53_12790"
     CDS             3781..4248
                     /gene="fabZ"
                     /locus_tag="VFO53_12790"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET8877200.1"
                     /translation="MSELDIGIIRRLLPHRYPMLLVDRVLDWESGKFVRGVKNVTVNE
                     PFFQGHFPTYPVMPGVLVIEAMAQVAGLLTMLSDVARRDGSQLVLFAGIDEARFKRQV
                     VPGDTLLLEAHLERAVRGIGRFRTKAMVGDELVCEAKLLAAIRDVPPTPPLPV"
     gene            4562..5338
                     /gene="lpxA"
                     /locus_tag="VFO53_12795"
     CDS             4562..5338
                     /gene="lpxA"
                     /locus_tag="VFO53_12795"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET8877201.1"
                     /translation="MPAIHPTALVDAGARIADDVAIGPYSIVGPDVSLGPGTRIGPHA
                     VITGRTSIGARNRVFQFASVGEIPQDRKYGGEPTSTVLGDDNVVREFVSIHAGTAQDR
                     ATTTIGNGNLFLAYTHVAHDCVVGNYTTFSNNAQIAGHVTIGDFVVLGAYAGVHQFCH
                     IGAHAMLAAGAIVLQDVPPYVTAAGYPAKPAGTNNEGLRRRGYSAEDIATVRRAYRTL
                     YRAGLSLEEARETLGLEAQAHPVVAPLVTFLSESRRGIVR"
     gene            5338..6516
                     /gene="lpxB"
                     /locus_tag="VFO53_12800"
     CDS             5338..6516
                     /gene="lpxB"
                     /locus_tag="VFO53_12800"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HET8877202.1"
                     /translation="MVPVTIGIVAGEASGDALAATLIEAVRQRRPAVRFAGIAGPKMH
                     AAGCEVLAPLDQLAVRGFSEVVAHLPALIALRRDIGRRFIRRRVPLFVGVDAPDFNLG
                     LERRLKRKRVRTVHFVSPSVWAWRRERLTTIGRSVDRMLALFPFEPPLYEAAGIAVTY
                     VGHPLAQDAARAGTRRATRETLKLKGPTPLFALLPGSRLSEIEMHAGLVLDAAARMLE
                     ARPDARFIVPLVSRTTRDAFIEARHRVGHDALPMTVLYGHAGDALRAADVALVASGTA
                     TLEAALARCPHVIFYRVSALTAHIVRRKLLVPWVGLPNVLAGRFVVPELLQDDASPGN
                     LAQAALNLFDDTVTRRRIETVFERMGRGLAVDTGALAAEAVVAELDRALADAAGRRGR
                     "
     gene            6518..7087
                     /gene="rnhB"
                     /locus_tag="VFO53_12805"
     CDS             6518..7087
                     /gene="rnhB"
                     /locus_tag="VFO53_12805"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004538183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET8877203.1"
                     /translation="MLVAGIDEAGRGPLAGPVVAAAVILDPKRKIRGLRDSKVMTADA
                     RDRVAAEIRTKSIAWAWAASDVGEIDALNILRATLLAMRRAVEALAVQPLEALIDGDH
                     CPELACPAYAIVKGDRDVPAISAASIIAKTTRDAMLVVLDREYPLYGFARHKGYGTPE
                     HLAALDLHGPCPAHRRTFAPVAQASFEFW"
     gene            7156..7671
                     /locus_tag="VFO53_12810"
     CDS             7156..7671
                     /locus_tag="VFO53_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET8877204.1"
                     /translation="MLLAIVQLSGLRDHFNVAFVRELVLRHPGGGLLLFVLLFSLGNL
                     IQIPGSVFLAAAVLTLGKLAGGAITYVAAVAGCAVTFVTIRALGGDALRLLDNRVAVR
                     ILRELDAHPVGSIAALRMLFQTAPAVNYALAMSGVRFRTYLIGTLIGLPLPIAAYCIF
                     FDVLATRLGLR"
     gene            7686..9335
                     /locus_tag="VFO53_12815"
     CDS             7686..9335
                     /locus_tag="VFO53_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877205.1"
                     /translation="MTIGTLRARRTGRIAVMVASAVVSTLLAMPASALTQIDFISDPD
                     DYVGGEQRFSLTPADGTISEAQGPGIQIYFQGSPSAVSPKTFWGITLAAPMGGTLMPG
                     NYASAHRFQSARRPQLDVFGDGRGCNTVAGKFTVYEAQFDADGNVVAFAADAEQHCEG
                     APAALRLRIRINSSVPLQLHLPQAMPGWAQEVYERALVTLDGSQSYDPDGHIVSWHWS
                     QIAGPAARIESPGVAITKLRAPDVAPGGADLVFRLDIDDDAGNHASGTSIVHVFDKRD
                     RRSLLTWRSPPGDYIGAGMPLMFTAADGAITVDNAYQGQTSVQASVAGADFDWWTLAF
                     AAPDGAPLAPGIYTNAERFPFQSPGVAGLSVDGAGRGCNTLAGRFTILEIDTPFRPTQ
                     FGARFGQRCEGAMPELRGTILVNAIAPGDPIARIGGAKSAARGAIVTLDGTGSSSSGS
                     MIVAYKWRQLSGPPITIDDPASSRISFVAPSTSGGPTRFELEVNDEDGLVDVAQVDVA
                     GLPTASVAIPAAGTPVVVLLAAAIGASGLLLMRRRRDRARP"
     gene            complement(9301..9702)
                     /gene="arfB"
                     /locus_tag="VFO53_12820"
     CDS             complement(9301..9702)
                     /gene="arfB"
                     /locus_tag="VFO53_12820"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HET8877206.1"
                     /translation="MTIPIDPGDIEWTAVRAQGAGGQNVNKVSNAVHLRFDVAASHLP
                     DPVKARLLALDDRRITREGVIVIKAQRHRSLERNYDDALARLSLIVDAAAVVPATRKP
                     SRPTRASQQRRVEAKVQRGRIKAARGRVDDA"
     gene            complement(9732..10475)
                     /locus_tag="VFO53_12825"
     CDS             complement(9732..10475)
                     /locus_tag="VFO53_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8877207.1"
                     /translation="MNDANARPLPLDVPLYKDVKRRLTDALTTGEWKPGAAIPAERRL
                     SERFRISVGTLRKAIDELVAENILIRQQGRGTFVASHNRERELFYFLHVVPQRGSKQY
                     PEVQLESFARGRADRRVSDALGIEPGDSVYRIRNLLRENGAPIIVDDITLPAQRFTGL
                     SERQFRERRSTVYNLYQEAFGVSVVATRERVRALPADEATAALLGVAVGTPLLSIRRV
                     ALSYREQPIEYRVSLVNTERHEYWAEIGT"
     gene            10679..10960
                     /locus_tag="VFO53_12830"
     CDS             10679..10960
                     /locus_tag="VFO53_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HET8877208.1"
                     /translation="MIHCVLHDPDDSVAVVVVEGIHAGTTLTGLILDEDRTIALECRQ
                     DIPIGHKVALKDMAVGDTVIKYGVDIGKVVQPIRKGEHAHVHNIKTKRW"
     gene            11150..12310
                     /locus_tag="VFO53_12835"
     CDS             11150..12310
                     /locus_tag="VFO53_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HET8877209.1"
                     /translation="MQNTFNGYRRQNGRVGVRNHVVILPVDDLSNAACEAVANNIKGT
                     LAIPHPYGRLQFGADLDLHFRTLIGTGSNPNVAAVVVIGIEEQWTKKVVDGIAATGKP
                     VQGFGIELHGDHDTIMRASKFAKEYVQWASEQTRAECALSDLWVSTKCGESDTTSGCG
                     SNPTVGDAFDKLHAAGCTLVFGETTELTGGEQIVAARCRDDKVRADFMRMFDRYQGVI
                     ERHKTSDLSDSQPTKGNIAGGLTTIEEKALGNIQKIGRKCTVDGVLDKAQMPTHPGLW
                     FMDSSSAAAEMVTLCAAAGFVVHFFPTGQGNVIGNPVLPVIKLCANPRTVRTMSEHVD
                     VDTSGLLQREMTMDEAGNKLLDMMFRTANGRLTAAEALGHREFVLTRLYESA"
     gene            12340..13368
                     /locus_tag="VFO53_12840"
     CDS             12340..13368
                     /locus_tag="VFO53_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877210.1"
                     /translation="MAASTRVLARTRSALTFAAAALSLAILPAAHAEMSEIHVSRQYG
                     ISYLPLMIMEDQKLIEKHAKTAGIDVKVDWSKFASGAVMNDALLSGNLQFASGGVAPF
                     TTLWAKTRSNLDVKAVAAINSMPLYLVTNNPKVKTIKDFTDNDKIALPAVKVSIQAVT
                     LQMAAEKAFGSGQEHKLDHLTVSMSHPDGETALLSGKSEVTAHLSSPPFQYQELEHKG
                     MHRVLNSYEVLGGPATFNVVWTTAKFHDDNPKVYAAFVAALDEATAQINGNKRAAAET
                     YLRISKDKDSLDDILKMLNDPEIKYTTTPNNVMKYVDFMHKIGSIKVKPDSWKDMYFP
                     NAQKLHGS"
BASE COUNT         2260 a         4511 c         4504 g         2173 t
ORIGIN      
        1 cgacgatcag ctcgctctcg ttctcgggca acaaggaatt cgacaccgac acgatcaaga
       61 aggcgctgaa ggacatcggc atcgccgagg cacgcatctt cgaccgctcg gcgctcgacc
      121 gcgcggagca ggagctgaag cgccagtaca tcacgcgcgg caagtacgcg gcgaacatcc
      181 agacgaccgt cacgccgcag gagcgcaacc gcgtcgcgat caacttcacg atcgacgagg
      241 gcgagtcggc gaagatcgcg cgcatcaaca tcgtcggcac gaaggcgttc accgagaagc
      301 agctgctctc ggagatggca ctgacgacgc cgggctggtt cacctggtac acgaagaacg
      361 accagtactc gaagcagaag ctttccgccg acctcgaggc gctgcgcagc ttctaccaga
      421 accggggtta cctcgagttc aacgtcgatt cgacgcaggt gtcgatcacg cccgacaagg
      481 aagacatcta catcacgctg aacatcaccg agggaccgcg cttcaatgtc ggcgacgtgc
      541 acatcgcggg cgacctcgtg gtgcccgaga gcgagctcgc gccgctcgtg cagctcaagc
      601 ccggtgacgt gttctcgcgc gccaagctgc aggcgtcggc gaaggacatc agcgaccgcc
      661 tcggcgcgga aggctacgcg ttcgccaacg tcaacgcggt gcccgagctc gaccgcgaca
      721 acgccaaggc gtcgttcacg ttctacgtcg atccgggccg gcgtgcctac gtgcgcaaga
      781 tcaacatcac cggcaactcg aagacccggg acgaggtgat ccggcgcgag ttccgccaga
      841 tcgaggacgc ctggtacgac gggccgcgca tcgagcggtc gaaggtgcgc gtgcgccggc
      901 tcaactactt cgacaacgtg agcgtcgaga cgcccccggt gccggggagt gccgaccagg
      961 tcgacgtcga ggtatcggtc accgagaagt cgaccggcaa cctgctcgcc ggcgtcggct
     1021 actcgagcga cgagggaatc gtgctcaacg cgtcgatctc ccaggacaac atcttcggca
     1081 ccggcaatgc gctgtcgctc ggcgtcaaca ccggccgctt caaccgcacg tattcgatgc
     1141 tgttcaccca gccctattac acggtggacg gcgtgtcgcg cacgctggag gcctacgaca
     1201 agagcctcga tccgaccggg ctcgcgatct ctcagtacgc gtccgacaca ttgggcaccg
     1261 cggtgggctt cggcgtgccg atcaccgaga ccgacacgat caattttggc ggccgctacg
     1321 agcacacgcg gatcacgctt ttcgacaaca gtccgccggt gtaccagcag ttcgtcaaag
     1381 acttcggcac cgcgtccaac gcgtacatcc tgactgccgg ctggtcgcgt gacacgcgcg
     1441 acgacgtgct gtacccgacg cgcggcaggc tgcagagcgt gctcgtcgaa accgggctgc
     1501 cattcggcga cctcgcgtat tacaaggtgc agtacacgca ttcggtctac tggccgctgt
     1561 acggtgatct cgtgctgatg gcgcgcggcg acttcggcta tggcggcggg ctgtcgggca
     1621 agccgctgcc gttcttcaag gctttcttcg cggggggtgt caattcggtg cgcggctatg
     1681 acacggcgtc gctcggaccg caggaccagt tcggcaacgc gctcggcggc cggcgcaaga
     1741 tcgtcggcaa cctcgaagct ttctatccga tcatcaaggg cgacaaggcg gtgcgggcca
     1801 gcgtgttcat cgacgcgggc gagatctacg cgaacggcca gcaatcgcag tttgagtcgt
     1861 ttcgctactc gaccggggtg ggcgtcgcct ggagctcgcc gatcggcccg ctcaagttca
     1921 gctatgccgt tccgctgaac gcgcgcgaag gcgaccggca acagcgcttc cagttccagg
     1981 ccggcgccgc gttctagcat gccgctttcc cgacccacat gagcatcatc gacagacagg
     2041 ccgtgctcgc gcgccttcgc acggctgccg tcgctctcgc actcgtcgcg ttcgcggcgg
     2101 cggcggcggc tgccgactac aagatcggct tcgtcaacac cgagcggttg ttccgcgaag
     2161 cgacaccggc caagcgcgcg caggcgaaga tcgaaaagga attcgcgacg cgcgacgccg
     2221 agatccagaa gctcgcgaag caggtgcgcg atctgcagtc ggcgctcgac cgcgacggcg
     2281 cgacgatgtc cgacgccgat cggcgcaaca gggagcgcga cctcgcgaac cagtcgcgcg
     2341 acctgacccg gatgcagcgc gagttccgcg aggatctcaa cctgcgccgc aacgaggagc
     2401 tcgccggcat ccaggagcgc gcgaacaagg tgatccagca gatcgctgca gacgagaagt
     2461 acgacctcat cctgcaggac cccgtcgtct acgcgagcca gcgcatcgac atcaccgacc
     2521 gggtgatcaa ggcgctcgcc gacaagtagc cgaaggcttc cggttcccca accccgcgca
     2581 ggtggcccgc acgatggctt cattgcgcac gctcgccggc aagccgctgt cggagctcgc
     2641 tgcattgacc ggcgcagcac tcgacggcga cgggagcgtc gtcgtgacgc gtgttgccac
     2701 gctcgagcgg gccgaacgcg gcgacatcgc gtttctcgcc aatccgagat atcgcggcca
     2761 gctcgctgcg acgcgggcca gtgccgtcat cgtcgacccc gcggccgcca cggcgacgcc
     2821 gctgccgaaa ctcgtcgctg cgaatccgta cgcagtctat gcgcgcgtcg ccgcgatcct
     2881 gcatcctgcg cccgcacccg cgccgggtgc ccattcgcac gcgtgcatcg acgcttcggc
     2941 aaccgtcgct gcatcggcaa gcgtcggccc gtacgccgtc atcgggcggc acgcgacgat
     3001 cggcgagcgc gccgccatcg gcgcccactg catcgtcggc gccggtgcga gcatcgccgc
     3061 cgatgtcgtg ctcgatgcgc gcgtcaccgt ctatcccggt tgcgtgatcg gcgcgcgtac
     3121 cctcgttcac agtggcgcgg tgatcggcgc cgacggcttc ggccttgccg aggaggacgg
     3181 acgctggctc aagatcacgc agatcggaca cgtcgtcatc ggcgccgacg tcgaaatcgg
     3241 cgccaacacg acgatcgatc ggggagcgat cgacgatacg atcatcgagg acggcgtcaa
     3301 gctcgacaac cagatacaga tcggacacaa ctgcgtgatc ggcgcgcata cggccattgc
     3361 cggctgcgtc ggcatcgcag gctcgacgcg gatcggcgcg cattgccgca tcggtggcgc
     3421 ggcgatgatc tcgggacacc tagagatcgc cgccggcagc gcgatctcgg ccgccaccct
     3481 ggtctacgat tcgatccgcg acccggggat atacactggc gcgtttccgg cgctgccgca
     3541 tcgtgaatgg aagcgcgtcg cgtcgcagat ccggcgcctg cgcgagctgg ccgagcgggt
     3601 gagcaggctc gaagctgcgc tgacggcgcg gcagaatgac aagaacgtgt agggcggttt
     3661 caagcggcga attacgccgc gtggggggca gcgcgtgagc ccgatgggcc gccccgaggg
     3721 cgaacagccg cgtgcgaagc acgagggccg tgcaagcggc cgcaagcgtg gggggattca
     3781 atgagcgaac tcgacatcgg catcatccgg cgccttctgc cgcatcgcta tccgatgctg
     3841 ctcgtcgatc gcgtgctcga ctgggagtcc ggcaagttcg tgcgtggggt gaagaacgtc
     3901 acggtcaacg agccgttttt ccagggccac tttccgacgt acccggtcat gcccggcgtg
     3961 ctcgtgatcg aggcgatggc ccaggtcgcc ggacttctca cgatgctctc cgacgtcgcg
     4021 cggcgcgacg gcagccaact cgtgctcttc gccggcatcg acgaggcgcg cttcaagcgg
     4081 caggtcgtgc ctggcgacac gctgctgctc gaagcgcacc tcgagcgcgc cgtgcggggc
     4141 atcgggcgct tcaggacgaa ggcgatggtc ggcgacgagc tcgtgtgcga ggcaaagctg
     4201 ctcgcggcga tccgcgacgt gccgccgacg ccgccgttgc cggtctaggg cctgttcacg
     4261 ctatgaatgc acgcgcgttg cacggccaaa acggccttgc gcgaggcgca aggccgaagg
     4321 cgggttgggc acccggcgag gtcttgcaac gatgcgcagg gccgttttgg cagcaactcg
     4381 aagggaacgg gtggcatggg gcgccactct tcgttaccct ccttgcgcag gcagccagcc
     4441 tgcgcttcgt caggcgcctc gattggcgcc ccatgccacc cgttcgcgcg cgcgttcata
     4501 gcgtgaacag gccctaggcc cgtcagcatc atggagccac gtcgcgtgcg cgaatcctga
     4561 cgtgccggcc atccatccca ccgcgctcgt cgacgccggc gcgcgcatcg ccgacgacgt
     4621 cgcgatcggg ccgtactcga tcgtcggccc cgacgtatcg ctcgggccgg gcacgcgcat
     4681 cggtccgcat gcggtgatca ccggccgcac gtcgatcggc gcgcgcaacc gggtcttcca
     4741 gttcgcctcc gtgggcgaga tcccgcagga tcgcaaatac ggcggcgagc cgacgtccac
     4801 cgtcctcggc gacgacaacg tcgtgcgcga attcgtgtcg attcacgcgg gcaccgcgca
     4861 ggaccgtgcg acgacgacga tcggcaacgg caacctgttc ctcgcgtaca cgcatgtcgc
     4921 gcacgactgc gtcgtcggca actacacgac gttctccaac aacgcgcaga tcgcgggtca
     4981 cgtgacgatc ggcgatttcg tcgtgctcgg cgcgtacgcg ggggtgcacc agttctgcca
     5041 tatcggcgcg cacgcaatgc tcgctgccgg agcgatcgtg ctgcaggacg tgccgccgta
     5101 cgtcaccgcg gccggttacc ccgcgaagcc cgccggaacg aacaacgagg ggttgcgccg
     5161 ccgcggatac tcggcggagg acatcgcgac cgtgcgccgg gcgtatcgca cgctctaccg
     5221 tgcgggcctg tcgctcgagg aggcgcgcga gacgctcggc ctcgaggcgc aggcacaccc
     5281 ggtcgtcgcg ccgctcgtga cgttcctttc ggaaagccgc cgcggcatcg tgcgctgatg
     5341 gtgcccgtca cgatcggcat cgtcgctggc gaggcgtcgg gcgacgcgct cgccgcgacg
     5401 ctgatcgaag cggtgcgcca gcgccgtccc gcggtgcgct tcgccggcat cgccggcccg
     5461 aagatgcatg cagccggctg cgaggtgctc gcgccgctcg accagcttgc ggtgcggggt
     5521 ttttcggagg tcgttgcgca tttgcccgcg ctgatcgcgt tgcggcgcga catcgggcgc
     5581 cgcttcatcc gccggcgcgt gccgctgttc gtcggcgttg acgcacccga tttcaatctc
     5641 ggcctcgagc ggcggctcaa gcggaagcgc gtgcgcaccg tgcatttcgt gagcccgtcg
     5701 gtgtgggcat ggcgccgcga gcgcctcacg accatcggac gctcggtcga ccgcatgctc
     5761 gcgctctttc cgttcgagcc gccactctac gaggcggccg gtatcgccgt cacgtacgtg
     5821 ggccatccgc tggcgcagga tgctgcgcgt gccggcaccc ggcgcgcaac gcgcgagacg
     5881 ctgaagctca agggcccgac accgctcttc gcgctgctgc ccggcagccg cctgtccgaa
     5941 atcgagatgc acgccgggct ggtgctcgac gccgcggcgc ggatgctgga agcgcgtccc
     6001 gatgcccggt tcatcgtgcc gctcgtgtcg cgcaccacgc gcgacgcgtt catcgaggcg
     6061 cgccaccgcg tcgggcatga cgcgttgccg atgacggttc tctacggaca cgcgggcgac
     6121 gcgctgcgcg ccgccgatgt cgcgctggtg gcttccggca ccgcgacgct cgaagcggcg
     6181 ctcgcgcgct gtccgcacgt gatcttctat cgggtgagcg cattgaccgc gcacatcgtg
     6241 cgccgcaagc tcctcgtccc atgggtcgga ttgccgaacg tgctcgccgg ccgtttcgtc
     6301 gtgcccgagc tcttgcagga cgacgccagt ccgggcaatc ttgcgcaggc cgcactcaat
     6361 ctgttcgacg acaccgtgac acgcagacgc atcgaaaccg tattcgagcg catgggacgc
     6421 gggcttgccg tcgacaccgg tgcgctcgcc gcggaggccg tcgtcgcgga gctcgatcgc
     6481 gcgctcgctg acgcggccgg ccggcgcggg cgatagcatg ctcgtcgccg gcatcgatga
     6541 ggcggggcgg ggtccgctcg cgggaccggt ggtcgcggcc gcggtcatcc tcgatccgaa
     6601 acgcaagatc cgcggcctgc gcgattcgaa ggtgatgacg gcggatgcgc gcgatcgcgt
     6661 ggcggccgag attcgcacga aatcgattgc ctgggcgtgg gcagcgagcg acgtcgggga
     6721 aatcgacgcg ctcaacatcc tgcgggcgac gctgctcgcg atgcgtcgcg ctgtcgaggc
     6781 gctcgcggtg cagccgctcg aggcgctgat cgacggcgac cattgcccgg agcttgcgtg
     6841 ccccgcctac gcgatcgtca agggcgatcg cgacgtgccg gcgatctcgg ccgcatcgat
     6901 catcgcgaag accacgcgcg atgccatgct cgtcgtgctc gaccgcgagt acccgctgta
     6961 cggcttcgcc cgtcacaagg gatacggcac gcccgagcac cttgcagcac tcgatctgca
     7021 tgggccttgt cccgcgcatc ggcgcacgtt tgcgccggtg gcccaggcat cgttcgagtt
     7081 ctggtaatcc gccgcttcgt cccggttttc gtcctgcgcc atcggcgcct gctcgcggtc
     7141 gttctctttc tggccgtgct gctcgcgatc gttcagcttt ccggcctgcg cgatcacttc
     7201 aacgtcgcat tcgtgcgcga gctcgtgctt cggcacccgg gcggcgggct tttgcttttc
     7261 gttctgctct tttcgctcgg caacctgata cagattccgg gatccgtttt cctcgccgcg
     7321 gcggtactga cgctcggcaa gctggccggg ggtgcgatca cctacgttgc ggcggtcgcg
     7381 ggatgcgccg tgacgttcgt gacgattcgc gcgctcggcg gcgacgcgct gcgattgctc
     7441 gacaatcggg tcgcggtccg gatcctgcgc gagctcgatg cccatccggt cggcagcatc
     7501 gccgcgttgc ggatgctgtt ccagacggcg ccggcggtga actacgcgct tgcgatgtcc
     7561 ggcgtccgct tccgcacgta cctcatcggg acgctgatcg gccttccgct gcccatcgcc
     7621 gcctactgca tcttcttcga cgtgctcgca acgcggctcg gcctgcgctg acaaggacaa
     7681 gcgccatgac gatcggaacg ctccgcgcga ggcgcacagg caggatcgcc gtgatggtgg
     7741 cgtccgcggt cgtgtcgacg ttgctggcga tgccagcgag cgcactgacg cagatcgact
     7801 tcatcagtga tccggacgat tacgtcgggg gcgaacaaag gttcagcctc accccggccg
     7861 acggcacgat ttccgaagcg cagggtccgg gaattcagat ctacttccag ggatcgccgt
     7921 ccgcagtgtc gcccaaaacc ttctggggca tcacccttgc cgcaccgatg ggtgggacgc
     7981 tgatgcccgg caattatgcg tcggcacatc gatttcagtc ggccaggcgg ccccagctcg
     8041 acgtgttcgg tgacggccgg ggatgcaaca cggtggccgg caaattcacc gtgtacgagg
     8101 cgcagttcga tgccgacggc aacgtcgtcg ccttcgccgc cgacgccgag cagcattgcg
     8161 aaggggcgcc ggctgcgctg cgcctgcgca tccggatcaa cagcagcgtg ccgctacagc
     8221 tgcacctgcc gcaggcgatg cccggatggg cgcaggaagt ttacgagcgc gcgctggtga
     8281 cgctcgacgg gtcgcagtcc tacgatcccg atgggcacat cgtgtcgtgg cactggtcgc
     8341 agatcgcggg accggcggca cgcatcgaat cgcccggcgt ggcaatcacc aagttgcgcg
     8401 cgcccgatgt cgcaccgggt ggcgccgatc tcgtcttccg gctcgacatc gacgacgatg
     8461 cgggcaatca tgcctccggg acaagcatcg tccacgtctt cgacaagcgc gaccggcgaa
     8521 gtctgctcac gtggcgcagc ccgccgggcg actatatcgg tgccggcatg ccgctcatgt
     8581 tcacggctgc cgacggagcg atcaccgtcg ataacgccta tcaaggccag acctcggtcc
     8641 aggcgagcgt tgccggcgcc gacttcgact ggtggaccct cgcgttcgcc gcgcccgacg
     8701 gcgcgccgct tgcgcctggc atctatacga acgcggagcg cttcccgttc cagagcccgg
     8761 gtgtcgccgg gctgtcggtc gatggcgcgg gccgcggctg caacacgctt gccggccgct
     8821 tcacgatcct ggagatcgac acgccgtttc ggcctacgca gttcggcgcc cgcttcgggc
     8881 agcgctgcga aggagcgatg ccggaactcc gcggcacgat cctcgtgaat gcgatcgccc
     8941 cgggcgatcc gatcgcccgg atcggcggag cgaagtccgc tgcgcgcggt gcaatcgtca
     9001 cgctcgacgg taccggttcg tcgtcatcgg gaagcatgat cgtcgcctac aagtggcggc
     9061 agctgtcggg gccgccgatc accatcgatg acccggcatc gtcccggatt tcgttcgtcg
     9121 cgccatcgac gtcgggcggg ccgacgcgat tcgagctcga agtgaatgac gaggacggac
     9181 tggtcgatgt cgcgcaagtc gacgtcgccg gactgccgac agcgtcggtc gcgatccctg
     9241 ccgcaggtac acccgtcgtc gtcctcctgg ccgcggcgat cggcgcaagc ggattgctgc
     9301 tcatgcgtcg tcgacgcgac cgcgcgcggc cttgatcctg ccgcgctgca ccttcgcttc
     9361 cacgcggcgc tgctgtgacg cgcgcgtcgg gcgcgagggc ttgcgcgtcg ccgggacgac
     9421 cgccgcggca tcgacgatca gcgaaaggcg cgcgagcgcg tcgtcgtagt ttcgctccag
     9481 gctgcgatgg cgctgcgcct tgatcacgat cacgccttca cgggtgatcc ttcgatcgtc
     9541 gagcgcaagc agccgtgcct tgaccggatc gggcaggtgc gacgcggcga catcgaagcg
     9601 caggtgcacc gcattggaca ccttgttgac gttctggcct ccggcccctt gcgcgcgcac
     9661 cgcggtccat tcgatgtcgc cggggtcgat cgggatggtc atcggtttcg cgggtcgcgg
     9721 cgcgcgacgc gtcacgtgcc gatctcggcc cagtattcgt gccgctcggt gttgacgagc
     9781 gacacgcggt actcgatcgg ctgctcgcga taggacagcg cgacgcggcg tatcgacaac
     9841 agcggcgttc cgaccgccac gccgagaagg gccgcggtcg cttcgtcggc aggcagcgca
     9901 cgcacgcgct cgcgcgtcgc gaccacggag acgccgaacg cttcctggta gaggttgtac
     9961 acggtcgagc ggcgctcgcg gaactgccgc tcggagaggc cggtgaagcg ctgcgccgga
    10021 agcgtgatgt catcgacgat gatcggcgca ccgttttcgc gaagcaggtt gcggatgcga
    10081 tagacgctgt cgcccggctc gatgccgagc gcgtcggaca cgcggcgatc ggccctgccg
    10141 cgggcaaacg attcgagctg cacctccggg tactgcttcg atccccgctg cggcacgacg
    10201 tgcaggaagt agaagagctc gcgctcgcgg ttgtggctcg cgacgaacgt gccgcgtcct
    10261 tgctggcgga tgaggatgtt ctcggcgacg agctcgtcga tcgccttgcg cagcgtgccg
    10321 acgctgatgc ggaagcgctc cgagagccgc cgctccgcgg gaatcgcggc gccgggcttc
    10381 cattcgccag tcgtcaaggc gtcggtgagc cggcgcttga cgtccttgta gagcggcacg
    10441 tcgagcggca gcggccgcgc attcgcgtcg ttcatggctg gcgtccgatg acgggcaggg
    10501 gcgggtgggc gcgcccgccc gggtgaaggg gtgcgggcac gctgcgtggg agtctagggg
    10561 gtgcgcaaaa attacgcaaa tgatgtaaat gatatagttg actcgacgcg gccggctcac
    10621 tacgatccgg ttatccaacc gcgccgggaa gacggcgcgc caactgccag gaactgccgt
    10681 gatccattgc gtattgcatg accccgacga cagcgttgcc gtcgtcgtcg tcgagggcat
    10741 ccatgccggg acgacgctca ccggactgat cctcgacgag gacaggacca tcgcgctcga
    10801 gtgccgccag gacatcccga tcggccacaa ggtcgcgctg aaggacatgg cggtcggcga
    10861 cacggtgatc aagtacggcg tggatatcgg caaggtcgtg cagccgatcc gcaagggcga
    10921 gcatgcgcac gtccacaaca tcaagaccaa gcggtggtga ttggcgttgt cggcgctacg
    10981 ttccgagcat gcggcgtgtt cggtggcgcg ggcctgcaag ccgttggagc gtcgcgcaaa
    11041 aaccgcgcga cgctccaacg gctcacagcc ccgcgtgacg cttcctgccg aactccatcc
    11101 ttcaacgcgt tgagcatcgc tcggcgcttt cctgagattc ctcatcgtca tgcagaatac
    11161 ttttaacggt taccggcgcc agaacggccg cgtcggcgtg cgcaaccacg tcgtcatcct
    11221 gcccgtcgac gatctgtcga acgcggcatg cgaggcggtc gcgaacaaca tcaagggcac
    11281 gctcgcgatc ccgcatccgt atgggcggct gcagttcggc gccgacctcg acctgcactt
    11341 ccgcacgctg atcggcaccg gcagcaatcc gaacgtcgcc gcggtggtgg tcatcggcat
    11401 cgaggagcag tggacgaaga aggtcgtcga cgggatcgcc gccaccggca agccggtgca
    11461 gggtttcggc atcgagctgc acggcgatca cgacacgatc atgcgcgcgt cgaagttcgc
    11521 gaaggaatac gtgcaatggg cgagcgagca gacgcgcgcc gaatgcgcgc tctcggatct
    11581 gtgggtgtcg acaaaatgcg gcgaatcgga taccacgtcg ggctgcggct cgaatccgac
    11641 ggtcggggac gcgttcgaca agctgcatgc cgccggctgc acgctggtgt tcggcgaaac
    11701 gaccgagctg acgggcggcg aacagatcgt tgcggcgcgc tgccgtgacg acaaggtgcg
    11761 cgccgacttc atgcgcatgt tcgatcgcta ccagggcgtc atcgagcggc acaagacgag
    11821 cgacctgtcc gactcgcagc cgacgaaggg caacatcgcg ggcggcttga cgacgatcga
    11881 ggagaaggca ctcggcaaca tccagaagat cggccgcaag tgcacggtcg acggcgtgct
    11941 cgacaaggcg cagatgccga cgcatccggg cctgtggttc atggacagct cgtcggcggc
    12001 cgccgagatg gtcacgcttt gcgccgcggc cggcttcgtc gtgcactttt tcccgaccgg
    12061 ccagggcaac gtgatcggca atccggtcct gccggtcatc aagctctgcg caaatccgcg
    12121 cacggtacgc acgatgagcg agcacgtcga cgtcgacacg agcgggcttc tgcagcgcga
    12181 gatgacgatg gacgaggcgg gcaacaagct cctcgacatg atgttccgca cggcgaacgg
    12241 ccgcctcacc gcggccgagg cgctcggcca tcgcgaattc gttctgacgc gcctctacga
    12301 gagcgcctga cgcttcacaa aaacaggaag gaggaggaaa tggcagcaag cacccgcgtc
    12361 cttgcgagga cgagatcggc cctgacgttc gcggcggcgg cgttgtcgct cgcgatcctg
    12421 cccgcggcgc acgccgagat gagcgaaatc cacgtgtcgc gtcagtacgg catcagctac
    12481 ctgccgctga tgatcatgga ggaccagaag ctgatagaga agcacgcgaa aaccgccggc
    12541 atcgacgtca aggtcgactg gtcgaagttc gcgagcggcg ccgtgatgaa cgacgcgctt
    12601 ctgtcgggca atctgcagtt cgcctcgggc ggcgtcgcgc cgttcacgac gctgtgggcg
    12661 aagacgcgca gcaatctcga cgtgaaggcc gtcgcggcga tcaattcgat gccgctctac
    12721 ctcgtcacga acaatccgaa ggtcaagacg atcaaggact tcaccgataa cgacaagatc
    12781 gcgctgcctg cggtgaaggt ctcgatccag gcggtcacgc tgcagatggc tgccgagaag
    12841 gcgttcggtt ccggacagga gcacaagctc gaccacctga cggtgtcgat gtcgcacccg
    12901 gacggcgaaa cggcgctgct ttccggcaag tccgaagtca ccgcgcatct gagctcgccg
    12961 ccgttccagt accaggagct cgagcacaag ggcatgcacc gcgtgctgaa ctcgtacgag
    13021 gtgctgggcg gaccggccac gttcaacgtg gtctggacga ccgcgaagtt ccacgacgac
    13081 aatccgaagg tgtatgcggc gttcgtcgcc gccctcgacg aggcgaccgc gcagatcaac
    13141 ggcaacaagc gggccgccgc cgagacgtac cttcgcatct cgaaggacaa ggacagcctc
    13201 gacgacatcc tgaagatgct gaacgatccc gagatcaagt acacgaccac gccgaacaac
    13261 gtgatgaagt acgtcgactt catgcacaag atcgggtcga tcaaggtgaa gcccgactcg
    13321 tggaaggaca tgtacttccc gaacgcccag aagctgcacg gcagctgatg ggtgcgggcg
    13381 agcgcggcct gcccgacctc gcggcgatcc gcgccgccca cgcgcgcatc gctccgtacg
    13441 tccaccgc
//
LOCUS       DASSVX010000297         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_30310, whole genome shotgun sequence.
ACCESSION   DASSVX010000297 DASSVX010000000
VERSION     DASSVX010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30310"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..656
                     /locus_tag="VFO53_12845"
     CDS             258..656
                     /locus_tag="VFO53_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877211.1"
                     /translation="MSIYGGRRGWNDLNDVVQPIVRPRPAEPRASVLKSATDWTSVRK
                     ARPADHLLPQSEKWFDTLPQGLAPCALASQFPRIVNLIVSQWNDHVNAPGLFEELLGD
                     RRGGRAGFPSAVRRDLLNLQEFWYSGKVDV"
     gene            complement(659..1441)
                     /locus_tag="VFO53_12850"
     CDS             complement(659..1441)
                     /locus_tag="VFO53_12850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET8877212.1"
                     /translation="MPTIDLDARVEFAASLARDAGALALRYFNRELAYSAESKGDPQD
                     WVSVADRSVEALCRARLAEAFPGDAMLGEETGGTIGDRTWVIDPIDGTLNFVHGVRYW
                     CVSIAFVERGVRRIAAIYDPPADELFTAASGRGARVNGRPMRTSDCRVLERALLVHGY
                     VYRHDLDAHLRLRRALIEAGAEVKDHGAGALMLAHVAAGRFDAYLEPHMHPWDASAGL
                     LLVEEAGGRVLSYPGDLGLRAGGAVLASAAGLYDRLFAMAGW"
     gene            1461..>2045
                     /locus_tag="VFO53_12855"
     CDS             1461..>2045
                     /locus_tag="VFO53_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET8877213.1"
                     /translation="MAESDTVAMRMRIGIDLGGTKTEIIVLDRDGNVRLRRREATPHN
                     DYRAIIDLVARLVHDAEEACGLAPGSATVGIGTPGSASRATGLLRGSNSVCMNGMPVK
                     RDIEARLARPIAMANDANCFALSEANDGAGRGAAVVFGVILGTGVGGGFVIDGGVLEG
                     INGIAGEWGHNPLPWPRDDEVPGPPCFCGRSGCIE"
BASE COUNT          352 a          686 c          680 g          327 t
ORIGIN      
        1 tggcgcaacg caatcacctc tacacgctcc aattcctgct gactcattcc ggtctccatc
       61 gcgccccctg cttgctggag gctggaacgt accggacatt tctactttgc taagaccgga
      121 cattagcgcg ttgccgcgac atgctcgctg ccatgacttg aatcgacccg ccttcccgtg
      181 gcatgcttcg ccccagcggc gcgccgtacg gccggtgcgc cacgcccgaa cgaatgcaac
      241 cgtgcggaga aaacgacgtg tcgatctacg gcggaagacg agggtggaac gatctcaacg
      301 acgtcgtgca gccgatcgtc cggccgcgcc ccgcggagcc acgtgcatcg gtgctgaagt
      361 cggccaccga ctggacgagc gtgcgcaagg cgcgacccgc cgatcacctg ctgccgcaaa
      421 gcgagaagtg gttcgatacg ctgccgcagg ggctcgctcc gtgcgcgctc gccagccagt
      481 tcccgcgaat cgtgaacctc atcgtttcgc aatggaacga tcacgtgaat gcgcctggac
      541 tgttcgagga gctgctcggt gaccgccgcg gtggccgtgc agggtttcct tcggccgttc
      601 ggcgcgacct gctgaacctg caggaattct ggtacagcgg gaaggtcgac gtgtgacctt
      661 accagccggc catcgcaaat agccggtcgt acagtccggc tgcgctcgcg agcaccgccc
      721 cgccggcacg caagcccagg tcaccagggt aggagagcac ccgcccgccc gcctcctcga
      781 cgagcagcaa acctgcgctc gcatcccacg gatgcatgtg cggctcgaga taggcgtcga
      841 agcgtccggc ggccacgtgc gcgagcatca gtgcgcctgc gccgtggtcc ttcacttcgg
      901 cgcctgcctc gatcaacgcc cggcgaagcc gaaggtgcgc gtcgagatcg tgacggtaca
      961 cataaccgtg cacgagaagc gcacgctcga ggacccggca gtcggacgtg cgcatcggcc
     1021 gcccgttgac acgcgccccg cggccggagg ccgccgtgaa gagctcatcg gcgggcggat
     1081 cgtaaatggc ggcgatgcgg cgcacgccgc gttcgacgaa cgcgatcgaa acgcaccagt
     1141 agcgcacgcc gtggacgaaa ttcagcgtgc cgtcgatcgg gtcgatgacc cacgttctgt
     1201 cgccgatcgt cccgccggtt tcctcgccga gcatcgcgtc gccaggaaac gcttccgcga
     1261 gccgcgcgcg gcaaagcgct tccacggaac ggtcggcgac gctgacccag tcctgcgggt
     1321 cgcccttgga ttcggcggaa taggcaagct cgcgattgaa atagcggagc gcgagcgcac
     1381 ccgcgtcgcg cgcgaggctc gccgcgaatt cgacgcgcgc gtcgagatcg atggtcggca
     1441 ttggcttgta tgatagccgc atggccgaaa gcgacaccgt ggcgatgcgc atgagaatcg
     1501 gcatcgacct cggtgggaca aagaccgaga tcatcgtgct cgatcgcgac ggcaacgtgc
     1561 gattgcgccg gcgcgaggcg acgccgcaca acgattatcg cgccatcatc gacctcgtgg
     1621 cgcggctcgt tcacgacgcg gaagaggcgt gcggcctcgc gccaggcagc gcaacggtcg
     1681 gcatcggcac gcccggctcg gcttcgcgtg ccacgggatt gcttcgcggc tcgaactcgg
     1741 tgtgcatgaa cggcatgccc gtcaagcgcg atatcgaagc gcgtcttgcg cgcccgattg
     1801 ccatggcgaa cgacgcgaat tgcttcgcac tttcggaggc gaacgatggc gcgggccgcg
     1861 gcgccgccgt cgtgttcggc gtgatcctcg gcacgggtgt cggcggcggc ttcgtgatcg
     1921 acggcggcgt tctggagggc atcaacggca tcgccgggga gtgggggcac aacccgttgc
     1981 cgtggccgcg cgacgacgaa gttcccggtc cgccttgctt ctgcggtcga tcgggctgca
     2041 tcgaa
//
LOCUS       DASSVX010000298         6730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_63417, whole genome shotgun sequence.
ACCESSION   DASSVX010000298 DASSVX010000000
VERSION     DASSVX010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63417"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="VFO53_12860"
     CDS             complement(<1..1335)
                     /locus_tag="VFO53_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET8877214.1"
                     /translation="MQSFDELGLRPEIMRAVTEAGYTTPTPIQAQAIPEILAGHDIMG
                     GAQTGTGKTAGFGLPILHKLAAHANASPSPARHPVRALILAPTRELAIQVEEQIKEYG
                     KYTSVRSTCVFGGVDIRQQLAIVRAGVEILVATPGRLLDHVEQKSVNLGQVEILVLDE
                     ADRMLDMGFIPDIKRIMRLLPAARQNLLFSATFSNEIKKLADELLNKPRLIEVARRNA
                     AAENVAQSAYKVPQEAKRALLVHLVTSRNVWQVLCFMRTKHGASRLARQLEKEGLAAS
                     AIHGDKTQVARLEALSQFKDGKLQVLVATDVAARGLDIDDLPLVVNYELPHVPEDYIH
                     RIGRTGRAGATGEAISFVAPEEEKHLVEIEKLLKKKIALCAAEGFDATAALASARERA
                     PRGASARGERTPRTANGSGNEHRALRREHAETGASRARPARAAVKRDTGIETP"
     gene            1603..3498
                     /gene="typA"
                     /locus_tag="VFO53_12865"
     CDS             1603..3498
                     /gene="typA"
                     /locus_tag="VFO53_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET8877215.1"
                     /translation="MSATRNDLRNIAIIAHVDHGKTTLVDKLLVQAGTFARHQHVAER
                     MMDNNDIERERGITIFAKNCAIRYPRGNEIVHINIVDTPGHADFGGEVERVLGMVDGV
                     LLLVDAVEGPMPQTRFVTQKALRQGLRPIVVVNKIDRPGARAEWVVNQTFELFDRLGA
                     TDAQLDFPVVYASALNGWATLDVGVAKAASATDAQNVVARPASADMRPLFETILAHVP
                     APAGQAEAPLQFQMSALDYSSYVGRLGIGRIRRGTLVAGQEVAIVDHALADGEIARRG
                     RVGQVFMFEGLNRVPVERAVAGDIVLVTGIDDLTIGTTLAAVDQPEALPPICVDEPTL
                     SMYFQVNTSPLAGREGKYVTSRNLRDRLMRELLTNMALRVEETGDTDAFLVSGRGELH
                     LTILIENMRREGYELAVSRPRVVMKEIDGVVCEPYETLSVDVEAQHQGAVMEALGVRR
                     GELADMASDAQGRARLDYRIPARGLIGFQGEFMNLTRGTGLMSHVFDAFAPVKGDIPE
                     RRNGVLISQEDGDAVAYALWKLQERGRMFVSPGDKLYEGMVIGIHSRDNDLVVNPIKG
                     KQLTNVRASGKDDAILLTPPIALTLEYAVEFIADDELAEITPKSIRIRKRFLKEHERK
                     RASREAA"
     gene            3628..4671
                     /locus_tag="VFO53_12870"
     CDS             3628..4671
                     /locus_tag="VFO53_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HET8877216.1"
                     /translation="MVCDALLDAQTASRFATLALANIARHYPHKLDHTMSSDADAQTP
                     RVLHPAFHGSYDWHSCVHMHWLLARVLRRFPALPERATIEALFDRQLHADAIAVEVAY
                     LARPESISFERTYGWAWLLKLAAEIAELRVAPWHEALAPLAGAFAGRFIEFLPRAHYP
                     VRYGMHANSAFALAFALDYADVARDTALADACRTKAIAWFAHDRDAPAQWEPSGTDFL
                     SPVLVEAELMRRVLDAATFAEWLAAFLPTLGEAQPAQLLTPATVTDRDDAQLVHLDGL
                     NLSRAWCFRGIARALPPDDPRVAVATAAAQRHLAAGWQGLASHAFVGAHWLASFAALA
                     LEGAGPGCYTRPP"
     gene            4668..6077
                     /gene="rmuC"
                     /locus_tag="VFO53_12875"
     CDS             4668..6077
                     /gene="rmuC"
                     /locus_tag="VFO53_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET8877217.1"
                     /translation="MIEYLLVAIVVALVVLIGLAAVALVRLAGLVEARDRAARDGAEL
                     RGLLEAFGRDAANHERDIRADLATARNESAAAGSALRKEVGETLSRFTQGTGQQLTGM
                     GALQQEQLKSFAERLAQLTQAWETRIESLRKTLEERLDVLRAENMVKLEQMRATVDEK
                     LQTTLEQRLGASFKHVSERLEQVHKGLGEMQTLASGVGDLKRVLTNVKSRGTWGEVQL
                     GALLGDLLPPGQFAQNVPTRPGSKDRVEYAIRFPGHGDDGTPCWLPIDAKFPLEDWQR
                     LQAAIEGADIEAVEASRRALEQFFKLQARSIRDKYVQAPHTTDFAILFVPTESLFAEA
                     MSRPGLADTLQRDFRVTIAGPTTLQALLNSFQLGFRTLAVQKRSTEVWRVLGAVKTEF
                     DRFGALLAKTKDKLLQVSNTLDEAGKKSTTIARKLRDVEALPEDEADRLLTGANGQVI
                     DLDDDTEALALSGPPDVAR"
     gene            6093..>6730
                     /locus_tag="VFO53_12880"
     CDS             6093..>6730
                     /locus_tag="VFO53_12880"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HET8877218.1"
                     /translation="MVFHAFRRFRRRRILRTSALPEALWRDACASLPFLAIYAPDELA
                     RLRDLVVLFLDAKSIVGARGHEVTPLQRTIIAIQACVLVLNLDLALYDGFENIVVYPG
                     EFVPGWEYEDEFGVVHKQDDALAGEAMHRGPVVLSWPDVEASADWDAAQMNLVIHEFA
                     HKIDMADGDANGCPPLPADMSRRDWQQTMQAAYDDFAARVDAGEDTAIDPYAA"
BASE COUNT         1094 a         2204 c         2292 g         1140 t
ORIGIN      
        1 gggtgtctcg atgcccgtgt cgcgcttcac cgccgcacgc gcaggccgtg cacgcgacgc
       61 gccggtttcg gcatgttccc ggcgtaaggc gcgatgctcg ttgccgctgc cgttcgcggt
      121 tctcggcgtg cgttcgccgc gcgcgctggc gccacgcggc gcccgctcgc gggcgcttgc
      181 cagggcagcc gtcgcgtcga agccctcggc ggcacaaagc gcaatcttct tcttgaggag
      241 cttctcgatt tcgacgaggt gcttctcttc ctcgggcgcg acgaacgaaa tcgcctcgcc
      301 ggtggcgcct gcgcgcccgg tgcgtccgat ccggtggatg taatcctccg gcacgtgcgg
      361 caactcgtag ttgacgacga gcggaaggtc gtcgatgtcg aggccgcgcg ccgcgacgtc
      421 ggtggcgacc agcacctgca gcttgccgtc cttgaactgc gacagcgcct cgaggcgcgc
      481 cacctgcgtt ttgtcgccgt gaatggccga cgcggcgaga ccttccttct cgagctggcg
      541 cgcaaggcgc gatgcgccat gcttggtgcg catgaagcac agcacctgcc agacgttgcg
      601 cgacgtgacg agatgcacga gcagcgcacg cttcgcctcc tgcggcacct tgtacgcgct
      661 ttgcgcgacg ttttcggccg cggcgttgcg acgcgccact tcgatgaggc gcggcttgtt
      721 caggagctcg tcggcgagct tcttgatctc attggagaac gtcgcggaga agagcaggtt
      781 ctgccgggcg gcgggcaaca gacgcatgat gcgtttgatg tcggggatga agcccatgtc
      841 gagcatgcgg tcggcttcgt ccagcacgag gatttcgacc tggccgagat tgacgctctt
      901 ctgctcgacg tgatcgagaa ggcgcccggg tgttgcgacg aggatctcga cgccggcgcg
      961 cacgatggcc agttgctggc ggatgtcgac gccgccgaac acgcaggtcg agcgcacgct
     1021 cgtgtacttg ccgtactcct tgatctgctc ctcgacctgg atcgcgagct cacgcgtcgg
     1081 cgcgaggatc agtgcgcgta ccgggtggcg ggcgggcgac ggactcgcat tcgcgtgcgc
     1141 cgcgagcttg tgcagaatcg gcaggccgaa tccggccgtc ttgccggtgc ctgtctgcgc
     1201 gccgcccatg atgtcgtggc cggcgaggat ttctggaatg gcttgcgcct ggatcggcgt
     1261 gggtgtggtg taaccggctt cggtcacggc ccgcatgatc tcgggccgca agccgagctc
     1321 gtcaaagctc tgcatgcagt tgctcctggt aacgcggcct accgcttcgg cggtttccgg
     1381 tccaggcagc ggaatgtcgt ttggctggga ttcgcgttgg gcgccgaatg caggaacacg
     1441 ggcgccggct ggcgggatgg gcggtgaccg gaacggccgc ccgaggacga attatagcct
     1501 aagcatcgga tttttcaaaa ttttcgtggc gaagagcatg cacggcacgc tataatcgac
     1561 tgttttgccg tgcgcatcga cgcgcggccg cgaaagcctc aaatgtccgc cacccgcaac
     1621 gaccttcgca acatcgcgat catcgcgcac gtcgaccacg gcaagaccac gctcgtcgac
     1681 aagctcctgg tccaggcggg cacgttcgcc cggcaccagc acgtggccga gcggatgatg
     1741 gacaacaacg acatcgagcg cgagcgcggt atcacgatct tcgcgaagaa ctgcgcgatc
     1801 cgctatccgc gcggcaacga gatcgtgcac atcaacatcg tcgacacgcc gggtcacgcc
     1861 gacttcggcg gcgaggtcga gcgcgtgctc ggcatggtcg acggcgtgct gctgctcgtc
     1921 gatgcggtcg aaggaccgat gccgcagacg cgcttcgtca cgcagaaggc gctgcggcag
     1981 ggcttgcggc cgatcgtcgt cgtcaacaag atcgaccggc cgggcgcgcg cgccgaatgg
     2041 gtggtgaacc agacgttcga gctcttcgac cggcttggcg caaccgatgc gcagctcgat
     2101 tttccggtcg tctacgcatc ggcgctgaac gggtgggcga cgctcgacgt cggcgtggcg
     2161 aaagctgcat cggcgaccga tgcgcaaaac gtcgtggcca ggccggcgag cgccgacatg
     2221 cgcccgctgt tcgagacgat cctcgcccac gtgcctgcgc cggcagggca agccgaggcg
     2281 ccgctgcagt tccagatgag cgcactcgat tattcgagct acgtcgggcg gctaggcatc
     2341 gggcgcattc gacggggcac gctggttgca gggcaggaag tcgcgatcgt cgaccatgcg
     2401 cttgccgacg gtgaaatcgc ccggcgggga cgcgtggggc aggtgttcat gttcgagggc
     2461 ctgaaccgcg ttccggtcga gcgtgccgtg gccggcgaca tcgtgctcgt caccggcatc
     2521 gacgacctca cgatcggcac cacgctcgcc gccgtcgacc agccggaggc actcccaccg
     2581 atctgcgtcg acgagccgac gctgtcgatg tacttccagg tgaatacgtc gccgctcgcc
     2641 ggccgcgagg gcaagtacgt cacgtcgcgc aatctgcgcg accggctgat gcgcgagctg
     2701 ttgaccaaca tggcgctgcg cgtcgaggaa acgggtgaca cggacgcgtt cctcgtttcg
     2761 ggccggggcg agctgcacct gacgatcctg atcgagaaca tgcgccgcga aggctacgag
     2821 ctcgcggtat cgcgtccgcg cgtcgtgatg aaggaaatcg acggcgtcgt ttgcgagccg
     2881 tacgagacgc tgtcggtcga cgtcgaggcc cagcatcagg gtgcggtgat ggaagcgctc
     2941 ggcgtgcgcc gcggcgagct cgccgacatg gcgtccgacg cgcagggacg cgcgcgtctc
     3001 gactaccgga ttccggcgcg cggcctgatc ggattccagg gcgagttcat gaacctcacg
     3061 cgcgggacgg gcctcatgag ccacgtgttc gacgcgttcg ctcccgtcaa gggcgacatt
     3121 cccgagcggc gcaacggcgt gctcatctcg caggaagacg gcgatgccgt cgcctatgcg
     3181 ctttggaagc tgcaggagcg cggacgcatg ttcgtgtcgc ccggcgacaa gctgtacgag
     3241 ggcatggtga tcggcatcca cagccgcgac aacgatctcg tcgtcaatcc gatcaagggc
     3301 aagcagctca cgaatgtgcg tgcatcgggc aaggacgacg cgatcctgct gacgccgccg
     3361 atcgcgctga cgctcgagta tgcggtcgaa ttcatcgccg acgacgagct cgccgaaatc
     3421 acgccgaagt cgatccgcat ccgcaagcgc ttcctcaagg aacacgagcg caagcgcgcg
     3481 tcgcgcgagg ctgcgtaggg gtcagatact attttcaccg cgtcgtttgc ggtgaaaatg
     3541 gtgtctgacc ccatttcgcg ttcaccgcgt cgtttgcggt gaaaatggtg tctgacccca
     3601 tttcggtccc atttcggttt ctcgaaaatg gtatgtgacg ctctgctcga cgcccaaacg
     3661 gcttcgcgct tcgcaacact cgcgcttgcg aacatcgcgc gacactaccc gcacaagctc
     3721 gatcacacga tgtcgagcga cgccgatgcg caaacgccgc gcgtgctgca tccggcattc
     3781 cacggcagct acgactggca ttcgtgcgtg cacatgcact ggctgctcgc gcgtgtcctg
     3841 aggcgctttc ccgcgctgcc cgagcgcgcc accatcgagg cgctcttcga tcgccagttg
     3901 cacgccgacg cgattgccgt ggaagtcgcg tatctcgcgc gccccgaaag catctcgttc
     3961 gagagaacgt acggatgggc gtggctgttg aagcttgccg cggaaatcgc cgagctgcgt
     4021 gttgcaccct ggcacgaggc actcgcgccg cttgccggcg cgttcgcggg gcgcttcatc
     4081 gagttcctgc cgcgtgccca ttacccggtc cgctacggca tgcacgcgaa cagcgcgttt
     4141 gcgcttgcgt tcgcgctcga ctatgccgat gtcgcccgcg acacggcact ggcagacgcc
     4201 tgccgaacga aggcaatcgc gtggttcgca cacgaccgcg acgcccctgc gcagtgggag
     4261 ccttcgggca ccgatttcct gtcgccggtg ttggtcgagg cggagctcat gcgccgtgtg
     4321 ctcgatgcgg cgacgtttgc cgaatggctc gccgcgttcc tgcccacgct cggcgaagcg
     4381 cagccggcgc agctgctcac gcccgcaacg gtcaccgacc gcgacgatgc gcagctcgtg
     4441 cacctcgacg ggctcaacct gtcgcgtgcg tggtgcttcc gcggcattgc ccgagcattg
     4501 ccgccagacg atccccgcgt tgccgttgcc acggccgcag cgcaacgtca tctggccgcg
     4561 ggctggcagg gtcttgcaag ccacgcgttc gtcggggcgc actggctcgc gagctttgcc
     4621 gcactcgcac tcgagggcgc tggtcccggc tgctacactc gcccgccatg atcgagtacc
     4681 tgctcgtcgc aatcgtcgtg gcgctggtcg tgctgatcgg ccttgcggcg gtggcgctcg
     4741 tacggctcgc cggtctcgtc gaggcgcgcg accgtgccgc gcgcgatggc gccgaactgc
     4801 gtggcctgct cgaggcgttc ggacgcgacg ccgccaatca cgagcgcgac atccgtgccg
     4861 acctcgcgac ggcgcgcaac gaatcggccg ccgcgggctc ggcgctgcgc aaggaagtgg
     4921 gcgagacgct ctcgcgcttc acgcaaggca ccgggcagca gttgacgggc atgggcgcgt
     4981 tgcagcagga gcagctcaag tcgttcgccg agcgcctcgc gcagctcacg caggcgtggg
     5041 agacgcgcat cgagtcgctg cgcaagacgc tcgaggagcg cctcgacgtg ctgcgcgcgg
     5101 agaacatggt caagctcgag cagatgcgcg cgacggtcga cgagaagctg cagacgacgc
     5161 tcgagcagcg cctcggtgcg tcgttcaagc atgtatcgga gcggctcgag caggtgcaca
     5221 aggggcttgg cgaaatgcag acgctcgcga gcggcgtcgg cgacctgaag cgcgtgctga
     5281 cgaacgtcaa gtcgcgcgga acgtggggcg aggtgcaact cggcgcactg ctcggcgacc
     5341 tgctgccgcc cggtcaattc gcgcagaacg tgccgacccg gccgggctcg aaggaccggg
     5401 tcgaatacgc aatcaggttt cccggccacg gcgacgacgg tacgccctgc tggctgccga
     5461 tcgatgccaa attcccgctc gaggactggc agcgattgca ggcggcgatc gagggcgcgg
     5521 acatcgaagc ggtcgaggcg agccggcgtg cgctcgagca gttcttcaag ctgcaggcac
     5581 gatcgattcg cgacaagtac gtgcaggcgc cgcacaccac cgacttcgcg atcctgttcg
     5641 tgccgacgga aagcctgttc gccgaggcga tgtcgcggcc gggccttgcc gatacgctgc
     5701 agcgcgactt ccgcgtcacg atcgccgggc ccaccacgtt gcaggctttg ctcaacagct
     5761 ttcaactcgg cttccgcacg ctcgccgtgc aaaagcgatc gaccgaagtg tggcgcgtgc
     5821 tcggcgccgt caagaccgag ttcgaccgct tcggggcgct gctcgcgaag accaaggaca
     5881 agctcctcca ggtcagcaac acgctcgacg aagcggggaa gaaaagcacg acgatcgcgc
     5941 gcaagctgcg tgacgtcgag gcgctgcccg aggatgaggc cgaccggctg ctcaccggcg
     6001 cgaacgggca ggtgatcgac ctcgacgacg acaccgaggc gcttgcgttg tcgggtccgc
     6061 cggacgtcgc gcgctagcgt cgcgctcggc gcatggtgtt ccacgcgttc cgtcggtttc
     6121 gacgcaggcg gatcctccgc acgtcggcgc tgcccgaggc gctatggcgc gacgcctgcg
     6181 cgtcgcttcc attcctcgcg atctacgcgc cggacgagct tgcgcgcctg cgcgatctcg
     6241 tcgtcctgtt tctcgacgcg aagagcatcg tcggcgcgcg ggggcacgaa gtcacgccgc
     6301 tgcagcgcac gatcatcgcc atccaggcct gcgtgctggt gctgaacctg gacctcgcgc
     6361 tgtacgacgg cttcgagaac atcgtcgtgt accccgggga attcgtcccc ggctgggaat
     6421 acgaggacga gttcggcgtc gtgcacaagc aggatgacgc gctggcaggc gaggcgatgc
     6481 accgcggccc ggtcgtgctg tcgtggccgg acgtcgaagc ctcggccgac tgggatgcag
     6541 cgcagatgaa tcttgtcatc cacgagttcg cgcacaagat cgacatggcc gacggcgacg
     6601 cgaacggttg tccgccgctg cctgccgaca tgtcgagacg cgactggcag caaacgatgc
     6661 aggcggcgta cgacgatttc gcggcgcgcg tggatgcggg cgaggatacg gcgatcgacc
     6721 cctacgccgc
//
LOCUS       DASSVX010000299         8188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_563539, whole genome shotgun sequence.
ACCESSION   DASSVX010000299 DASSVX010000000
VERSION     DASSVX010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8188
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563539"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1322
                     /locus_tag="VFO53_12885"
     CDS             114..1322
                     /locus_tag="VFO53_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877219.1"
                     /translation="MKRSLVALTIAGAFAAGTAQAQFTGNVIKIGVLSDMSSLYTDLA
                     GAGSVVAAKMAVEDSGIEKRGIKVEIVSADHQNKADVGSAIARRWYDAEGVDVIVDVP
                     NSGVALAVSQITKDKNKVFLASGPGSSDLTGKACTPNTVHWTYDTWMLANGTGTAITK
                     HGGNTWFFITADYAFGQALERDTTAAVEKAGGKVLGGVKVPINNQDFSSFLLQAQASK
                     AKVIGLANAGGDTTNSIKQAAEFGIVKGGQSLAGLLVFITDVHSLGLPTAQGLTFTET
                     FYWDANDKTRAFSTRFAPQDKGIHPTMVHAGVYASLLHYFKAVEALKSDADGAKIVAK
                     MKEMPTDDPLFGKGSIRVDGRTLHPAYLVEVKKPSESKGPWDYYKTLATIPADEAFRP
                     LKDGGCSLVK"
     gene            1463..2326
                     /locus_tag="VFO53_12890"
     CDS             1463..2326
                     /locus_tag="VFO53_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8877220.1"
                     /translation="MQALFGQLLIGLINGAFYAMLSLGLAVIFGLLNIINFTHGAQYM
                     MGAFCAWFLLNKAGIGYWWALLLAPIGVGIVGLVIERTMLSRLYKLDHLYGLLLTFGL
                     ALVIQGLFRNEFGSSGLPYQIPSQLTGGINLGFMFLPIYRAWVIVASLVICLATWFVI
                     ERTKLGSYLRAATENPALVQAFGINVPRMITLTFGFGVALAAVAGVMAAPIYQVNPLM
                     GADLIIVVFAVVVIGGMGSILGAILTGFGLGIVEGLTKYFYPEASSTVIFVIMVIVLL
                     VRPAGLFGKER"
     gene            2335..3294
                     /locus_tag="VFO53_12895"
     CDS             2335..3294
                     /locus_tag="VFO53_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8877221.1"
                     /translation="MAQTAARKAASRNATLVPTLVMAVVLAVAPFAGIYPVFILKALC
                     FALFACAFNLLLGFGGLLSFGHAMFLGTAGYVTAHAAKEWGVTPEIAIVAGTVASAGL
                     GVVVGALAIRRQGIYFAMITLAIAQMMYFFYVQAPFTHGEDGIQAVPRGHLFGLIDLS
                     RPLAMYYTVLVVFIAAFALIVRIVHSPFGQVLKAIRENEPRAISLGYDADRYKLLAFV
                     LSATLAGLAGALKAIVFQLASLTDVYWTMSGEVVLMTLLGGMGTIFGPVVGAFVIVAL
                     ENYLAGFGEWVTIITGVIFVVCVLAFRRGIVGELTAWWAARRS"
     gene            3294..3854
                     /locus_tag="VFO53_12900"
     CDS             3294..3854
                     /locus_tag="VFO53_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877222.1"
                     /translation="MRVLAGLAGVLVVAQGLAAADEALHASSGCRAGRPQGPYQLRGD
                     NGQLRVAGAFNDGTRTGSFIFWRANGVREAHVPYDRGVRNGTVATWYAGPPGREPARH
                     FESVWRHGRRDGETRSWYPDGGRRSVAEYTNGRLVASAGWTAAGEPLAEAAARDQALR
                     DERAADAEYATYSALIRDHMPSCGTA"
     gene            3927..5228
                     /locus_tag="VFO53_12905"
     CDS             3927..5228
                     /locus_tag="VFO53_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HET8877223.1"
                     /translation="MAHAWQDRAKALDAPGALAGIAARAARASIVLIGEASHGTEDFY
                     AMRAALTRRLIADHGFRAITLEADWPDTFRAHRYVTGRSDDKDAATALSDFRRFPAWM
                     WRNETMREFVDWLAGFNNARDSAERVGLFGLDLYSLYGSIESVLVYLDRVDPDAAARA
                     RARYRCFEFFGDDPQGYALSVAQGVDSCEEEAVAQLVELRRHAMLPLKGVTRDDEDVF
                     SAEQNARLIVNAERYYRSMYRGRESTWNLRDRHMVDTLDALRERLPGASDGTVVWAHN
                     SHLGDARATEMSRRGEVNVGQLVRERYGRDVFSIGFSTYDGTVTAASDWDAPAERKRV
                     RPGLAGSYERTFHESGQDRFWVDLGEREVATGLRGALLQRAIGVIYRPETERWSHYFE
                     ARLPEQFDAMIHLDNTSALTPLDRTAGWDEGEPPETWPTGM"
     gene            5331..6293
                     /locus_tag="VFO53_12910"
     CDS             5331..6293
                     /locus_tag="VFO53_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET8877224.1"
                     /translation="MQRLIRIVAASCLALCAASALAAYPERPVTLIVPWGAGGGTDAT
                     ARIIGTLLEKELGQPVNVVNRTGGSGVVGHAAIAGAPPDGYTIGMITVEIGMMHWVGL
                     TQLSGASYTPIGIVNADPAGLQVRADAPYKNANDLVAAIKANPGKFKASGTGQGGIWQ
                     LAIAGMLRDMKVDPASVPWVPSNGAAPGLQDLVAGGIEIVPCSLPEARSLIDAGKVKS
                     LAVMADKRAAMYPDLPTLKEETGSNWTMAAWRGIAAPKGLPNDVADKLAAAIRKIVAS
                     KDYTDFMAKQGYGVIYEGPADMAKFMAKSDADLGATMKAVGIAK"
     gene            6304..6786
                     /locus_tag="VFO53_12915"
     CDS             6304..6786
                     /locus_tag="VFO53_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HET8877225.1"
                     /translation="MKLNDAVWGALVLLLGIAILVAIRNFPTIPGQQYGPALFPGVIA
                     VGLIVCGGVLVYRHLALSSPQTRARWVTWDDWVHSPRHVVAFAVVVAVNVLYIALVDR
                     LGFVLTAVAYLSALFAVFRVRARWVVPLAVVVTLIIHYAFYKLLRVPLPWGVLQGVAW
                     "
     gene            6824..>8188
                     /locus_tag="VFO53_12920"
     CDS             6824..>8188
                     /locus_tag="VFO53_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HET8877226.1"
                     /translation="MSDAWLQAFALVFQPYNMVVMLGASLFGLFVGAVPGLTATMATA
                     LLVPVTFFMAPIPAVAAIVTATAMAIFAGDIPGCLMRMPGTPASAAYTDEAYAMTRKG
                     QAELALGAGLVFSATGGLFGTLVLIVAAPTLANFALSFSSFEYFWLVLLGLTCAVFIT
                     SDRPLKGFVSLLLGLLVASVGLNNPAAIPRFTFGSVELLGGIELIPMMIGMFAISEII
                     RYAVDTNAQVHLGERAIGNVFKGIWGLTKKYPVQQLRGSVLGTLVGALPGAGGDIAAW
                     MAYAVSKKWSKEPEKFGTGHVEGIVEAGAANNAALAGAWIPALVFGIPGDSITAIVIG
                     VLYMKNMNPGPTLFINNPQNIYAVYLLFIIANLIMIPLGFLCIKAAKNILLVPRSMLM
                     PVIMLFCVVGSFAINNTAFDVGVMLIAGFVAFVLENNGFPIAPAILGVVLGGMLEQNF
                     VTSMI"
BASE COUNT         1253 a         2699 c         2774 g         1462 t
ORIGIN      
        1 gctgcacgag tacctgggtg tttagggggg tcggagacga cttttgcgga gttcggaaaa
       61 agtcgtctct gaccccactt cggtttttca caggaggagt accgatgaag gcaatgaaac
      121 gcagtctcgt tgcgctcacg atcgccggcg cattcgccgc cggcaccgcg caggcgcagt
      181 tcaccggcaa cgtgatcaag atcggcgtgc tgtccgacat gtcgagcctc tacaccgacc
      241 tcgccggcgc gggctcggtc gtggcggcga aaatggccgt cgaggattcg ggcatcgaga
      301 agcgcggcat caaggtcgag atcgtctcgg ccgaccatca gaacaaggcg gacgtgggct
      361 cggcgatcgc gcgccgctgg tacgacgccg aaggcgtcga cgtgatcgtc gacgtgccga
      421 actcgggcgt ggcgctcgcg gtgtcgcaga tcaccaagga caagaacaag gtgttcctcg
      481 cgtcgggccc cggctcgtcc gacctgacgg gcaaggcgtg cacgccgaac accgtgcact
      541 ggacctacga cacgtggatg ctcgcgaacg gcacgggcac cgcgatcacg aagcatggcg
      601 gcaacacctg gttcttcatc accgccgatt acgcgttcgg gcaggcgctc gagcgcgaca
      661 cgaccgcggc ggtcgagaag gccggcggca aggtgctggg tggcgtgaag gtgccgatca
      721 acaaccagga cttctcgtcg ttcctgctgc aggcgcaggc gtcgaaggcg aaggtgatcg
      781 gccttgcgaa cgcgggcggc gacacgacga actcgatcaa gcaggcagcc gaattcggca
      841 tcgtgaaggg cggccagagc ctcgcgggcc tgctcgtgtt catcaccgac gtccactcgc
      901 tcggcctgcc gaccgcgcag ggtctcacgt tcaccgagac gttctactgg gatgcgaacg
      961 acaagacccg cgcgttcagc acgcgcttcg cgccgcagga caagggcatc cacccgacga
     1021 tggtgcacgc cggcgtctac gcgtcgctgc tgcactactt caaggcggtc gaggcgctga
     1081 agagcgacgc cgacggcgcg aagatcgtcg cgaagatgaa ggagatgccc accgacgatc
     1141 cgctgttcgg caagggatcg attcgcgtcg acggccgcac gctgcacccg gcgtatctcg
     1201 tcgaggtgaa gaagccgtcc gaatcgaagg gaccgtggga ctactacaag acgctggcca
     1261 cgattccggc cgacgaggcg ttccggccgc tgaaggacgg cggctgctcg ctcgtcaaat
     1321 agcgcgtcat catccgtcgc gccggcatgt gcaatcgctt cgacccggca tcgacacggc
     1381 gcggcggata actgctcgcg ctcgagcgtg cgccttgcgg cgccgctcga cgcttcgcac
     1441 cccaacgcat cggcttcgct gaatgcaggc acttttcggg caactgctga tcggcctcat
     1501 caacggcgcg ttctacgcga tgctgtcgct cgggctcgcc gtcattttcg ggctcctcaa
     1561 catcatcaac ttcacgcacg gcgcgcagta catgatgggc gcgttctgcg cgtggttcct
     1621 gctgaacaag gcgggcatcg gctactggtg ggcgctgctg ctcgccccga tcggcgtcgg
     1681 catcgtcggt ctcgtcatcg agcgcacgat gctgtcgcgt ctctacaagc tcgaccacct
     1741 gtacgggctg ctgctcacgt tcgggctcgc ccttgtgatc cagggattgt tccgcaacga
     1801 gttcggctcg tcgggcctgc cgtaccagat cccgtcgcaa ctcaccggcg gcatcaacct
     1861 cggcttcatg ttcctgccga tctaccgcgc ctgggtgatc gtcgcctcgc tcgtcatctg
     1921 tctcgcgacc tggttcgtca tcgagcgcac caagctcggc agctatctgc gggcggccac
     1981 cgagaacccc gcgctggtgc aggccttcgg catcaacgtg ccgcggatga tcacgctgac
     2041 cttcggcttc ggcgttgcgc tggccgcggt cgccggcgtc atggcggcgc cgatctacca
     2101 ggtgaatccg ctgatgggtg ccgacctcat catcgtcgtc ttcgcggtcg tcgtgatcgg
     2161 cggcatgggc tcgatcctcg gcgccatcct caccgggttc ggcctcggca tcgtcgaggg
     2221 tctgaccaag tatttctacc ccgaagcgtc gagcaccgtc atcttcgtga tcatggtcat
     2281 cgtgctgctc gtgcggccgg cgggtctgtt cgggaaggag cggtagtggc cgcgatcgcg
     2341 cagaccgccg cgcgcaaggc cgcgtcacgc aacgcgacgc tggtgccgac gctcgtgatg
     2401 gcggtggtgc tcgccgtcgc gccgttcgcc ggcatctacc cggtgttcat cctgaaggcg
     2461 ctgtgcttcg cgctgttcgc gtgcgcattc aacctgctgc tcggcttcgg cggactgctg
     2521 tcgttcgggc atgcgatgtt cctcggcacg gcgggctacg tcaccgcgca tgcggcgaag
     2581 gaatggggcg tgaccccgga gatcgcgatc gtggccggca ccgtggcgtc ggccggcctc
     2641 ggcgtggtgg tcggcgcgct cgcgatacgc cggcagggca tctatttcgc gatgatcacg
     2701 ctcgcgatcg cgcagatgat gtatttcttc tacgtgcagg cgccgttcac gcacggcgag
     2761 gacggtatcc aggcggtgcc gcgcgggcat ctgttcggcc tgatcgacct gtcgcgcccg
     2821 ctcgccatgt attacacggt gctcgtcgtg ttcatcgccg cgttcgcgct catcgtccgc
     2881 atcgtccatt cgccattcgg acaggtgctg aaggcgatcc gcgaaaacga gccgcgggcg
     2941 atctcgctcg gctacgatgc cgatcgctac aagctgctcg cattcgtgct gtcggcgacg
     3001 ctcgcgggcc tcgcgggcgc actcaaggcg atcgtgttcc agcttgcatc gctcaccgac
     3061 gtctactgga cgatgtccgg cgaggtcgtg ctgatgacgc tgctgggcgg catgggcacg
     3121 atcttcgggc cggtcgtcgg cgcgttcgtg atcgtcgcgc tcgagaatta cctcgcgggt
     3181 ttcggtgaat gggtgacgat catcaccggc gtcatcttcg tcgtctgcgt gctcgcgttt
     3241 cgccgcggca tcgtcggcga gctcacggcg tggtgggcgg cgcgtcgatc gtagtgcgcg
     3301 tgctcgccgg ccttgccggc gtgctggtcg tggcgcaggg tcttgcggcg gccgacgagg
     3361 cgcttcacgc gagcagcggc tgccgggcgg gccggcccca gggaccgtat caactgcgcg
     3421 gcgacaacgg ccagttgcgc gtcgcggggg cgttcaacga cggcacgcgc acgggctcgt
     3481 tcattttctg gcgcgcgaac ggcgtgcgcg aggcgcacgt gccctacgac cgcggcgtac
     3541 gcaatggcac cgtcgccacg tggtacgccg gcccgccggg ccgcgagcct gcgcggcact
     3601 tcgagtcggt gtggcgccac ggccgccgtg atggcgagac gcgctcgtgg tatcccgatg
     3661 gcggccgccg ttccgtagcc gagtacacga acgggcgcct cgtcgcgagt gccggctgga
     3721 cggccgcggg ggagccgctt gccgaggcgg ccgcgcgcga tcaggcgctg cgcgacgaac
     3781 gcgccgcgga tgccgaatac gcaacctaca gtgcgctgat ccgcgatcac atgccgagct
     3841 gcggtacggc gtaacgcctc ggcaccaacg ttccccgccg cggtccaagc catcgtcctc
     3901 aatcggcctt gccagaggag gttgcgatgg cgcatgcgtg gcaggatcgc gcaaaggcgc
     3961 tcgacgcacc cggtgcgctc gccgggatcg cggcgcgcgc cgcacgcgcg tcgatcgtgc
     4021 tgatcggcga ggcgtcgcac ggcaccgagg atttttatgc gatgcgggcg gcgctcacgc
     4081 gccggctcat agccgaccac ggatttcgcg caatcacgct cgaggccgac tggcccgaca
     4141 cgttccgggc gcaccggtat gtcaccggtc gctccgacga caaggacgcg gcgaccgcgc
     4201 tttccgattt ccgtcgcttt ccggcgtgga tgtggcgcaa cgagacgatg cgcgagttcg
     4261 tcgactggct cgccgggttc aacaacgcgc gtgacagcgc cgagcgcgtc ggcctgttcg
     4321 gactcgacct ttacagcctg tacggctcga tcgagtcggt gctcgtctat ctcgatcgcg
     4381 tcgatccgga cgccgccgca cgcgcccgcg cgcgctaccg ctgcttcgaa tttttcggcg
     4441 acgacccgca gggctacgcg ctctcggtcg cgcagggcgt cgattcctgc gaagaagagg
     4501 ccgttgcgca actcgtcgag cttcgccgcc acgcgatgct gccgctcaag ggcgtgacgc
     4561 gcgatgacga ggacgtcttc tctgccgagc agaacgcgcg cctcatcgtc aacgccgagc
     4621 gctattaccg ctcgatgtac cgcggccgcg aatcgacgtg gaatctgcgt gaccggcaca
     4681 tggttgacac gctcgatgcg ctgcgcgagc gcctgcccgg cgcgtccgac ggcacggtcg
     4741 tgtgggcgca caactcgcat cttggcgatg cgcgcgcaac cgaaatgagc cgccgcggcg
     4801 aagtcaacgt cgggcagctc gtgcgcgagc gatacggccg cgacgtgttc tcgatcggct
     4861 tttcgacgta cgacggcacg gtcaccgccg cgtccgactg ggacgcaccg gccgagcgca
     4921 agcgtgtgcg gcccggcctt gcgggcagct acgagcgcac gttccacgag agcggccagg
     4981 atcgcttctg ggtcgacctc ggcgagcgcg aggtcgccac gggcttgcgc ggcgcgctgc
     5041 tgcagcgcgc gatcggcgtg atctaccgcc cggaaacgga gcgctggagc cactacttcg
     5101 aggcgcgctt gcccgagcag ttcgacgcga tgatccatct cgacaacacc tcggcgttga
     5161 cgccgctcga ccgcaccgcc ggctgggacg aaggcgaacc ccccgagacg tggcccaccg
     5221 ggatgtgaca gaacgttgct tgaccgatct cgacgccggc tgctaaggtc gggcggggaa
     5281 aaacgcgtcg gcgcgtcctg cgcccgtcgc gtgcgttcac gggaggagtc atgcagcgtc
     5341 tcattcgaat cgtcgcggcg tcgtgccttg cactgtgtgc agcgagcgcg cttgccgcct
     5401 atcccgagcg tccggttacg ctgatcgtgc cgtggggtgc cggcggcggc acggacgcga
     5461 ccgcgcgcat catcggcacg ctgctcgaaa aggagctcgg ccagccggtg aacgtggtca
     5521 accgcaccgg cggcagcggt gtcgtcggcc acgcggccat tgcaggcgca ccgcccgatg
     5581 gctacacgat cgggatgatc accgtcgaga tcgggatgat gcactgggtc ggtctcaccc
     5641 agttgagcgg cgcgtcgtac acgccgatcg gtatcgtcaa cgccgacccc gccggcctgc
     5701 aggtgcgcgc cgatgcgccg tacaagaacg cgaacgacct cgtcgcggca atcaaggcca
     5761 atcccggcaa gttcaaggcc tccggcacgg ggcagggcgg catctggcaa ctcgcgatcg
     5821 ccggcatgct gcgcgacatg aaggtcgatc ccgcaagcgt gccctgggtg ccgtcgaatg
     5881 gcgcggcgcc gggattgcag gacctcgttg ccggcggcat cgagatcgtg ccgtgctcgc
     5941 tgcccgaagc ccgttcgctg atcgatgccg gcaaggtaaa aagcctcgcc gtgatggccg
     6001 acaagcgcgc ggcgatgtac cccgacctgc cgacgctcaa ggaagaaacc ggaagcaact
     6061 ggacgatggc ggcctggcgc ggcattgcgg cgccgaaggg tttgccgaat gacgtggccg
     6121 acaagcttgc ggcggcgatc aggaagatcg tggcgagcaa ggactacacg gacttcatgg
     6181 cgaagcaagg gtacggcgtg atctacgagg gtcccgccga catggcgaag ttcatggcga
     6241 aatcggacgc cgatctcggc gcgacgatga aagcggtcgg catcgcgaag tgatcggccg
     6301 cgcgtgaagt tgaacgacgc cgtctggggc gcgctcgtcc tgctgctcgg gatcgcgatt
     6361 ctcgtcgcca tccgcaactt cccgacgatt cccgggcaac agtacggtcc cgcgctgttc
     6421 ccgggcgtca tcgcggtcgg gctcatcgtt tgcggcgggg tgctcgtcta caggcatctc
     6481 gcgctttcgt cgccgcaaac gcgagcgcgt tgggtgacgt gggacgactg ggtgcattcg
     6541 ccgcgccacg tcgtcgcgtt cgccgtcgtc gtcgccgtga acgtgctgta catcgcgctc
     6601 gtcgaccgcc tcggcttcgt cctcaccgcc gtcgcctact tgagcgcatt gttcgccgtc
     6661 ttccgcgtgc gtgcgcgctg ggtggtgccg ctcgcggtcg tggtcacgct gatcatccac
     6721 tacgcgttct acaagctgtt gcgggtgccg ctgccctggg gcgtgctgca gggcgtcgcg
     6781 tggtgattgt cgcggcgcgg cgcacagtcg ctgaggttca gccatgagtg acgcgtggct
     6841 gcaggcgttt gcgctcgtgt ttcagccgta caacatggtg gtgatgctcg gcgcgtcgct
     6901 gttcggactg ttcgtcggcg cggtgcccgg cctcaccgca acgatggcca cggcgctgct
     6961 cgtgcccgtc acgttcttca tggcgccgat tcccgccgtg gctgcgatcg tcaccgcaac
     7021 cgccatggcg atctttgccg gcgacattcc cggatgcctg atgcgcatgc ccggcacgcc
     7081 ggcgtccgcc gcctacaccg acgaggcgta cgcgatgacg cgcaaggggc aggcggagct
     7141 cgcgctcggc gccggtctcg tcttctcggc caccggcggc ctcttcggca cgctcgtgct
     7201 gatcgtcgcc gcgccgacac ttgccaactt tgcgttgagc ttcagctcgt tcgagtattt
     7261 ctggctggtg ctgctcggcc tcacctgcgc ggtgttcatc acgtccgacc ggccgctcaa
     7321 gggcttcgtg tcgctgctgc tcgggctgct cgtcgcgtcg gtcggcctca acaatccggc
     7381 cgccattcca cgcttcacgt tcggtagcgt cgagcttttg ggtggcatcg agctgatccc
     7441 gatgatgatc ggcatgttcg cgatctccga aatcatccgc tacgcggtcg acacgaatgc
     7501 gcaagtgcac ctcggcgagc gggcgatcgg caacgtgttc aaggggatct ggggtctgac
     7561 gaagaagtat ccggtgcagc agctgcgcgg cagcgtgctc ggcacgctcg tcggcgcgct
     7621 gccgggcgcg gggggcgaca tcgccgcgtg gatggcgtat gcggtgagca agaagtggtc
     7681 gaaggagccg gagaaattcg gcaccggaca cgtcgagggc atcgtcgagg cgggtgctgc
     7741 gaacaacgcg gcgctcgccg gcgcgtggat accggcgctg gtgttcggca tcccgggtga
     7801 ctcgatcacc gcgatcgtca tcggcgtgct gtacatgaag aacatgaacc ccggcccgac
     7861 gctgttcatc aacaacccgc agaacatcta cgcggtgtac ctgctgttca tcatcgcgaa
     7921 cctgatcatg attccgctcg gattcctgtg catcaaggcg gccaagaaca tcctgctcgt
     7981 gccgcgcagc atgctgatgc cggtgatcat gctgttctgc gtcgtcggct cgttcgcgat
     8041 caacaacacg gcgttcgacg tcggcgtgat gctgatcgcg ggcttcgtcg cgttcgtgct
     8101 cgaaaacaac ggcttcccga tcgcgccggc catcctcggc gtcgtgctcg gcggcatgct
     8161 cgagcagaac ttcgtcacgt cgatgatc
//
LOCUS       DASSVX010000300         5319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1263643, whole genome shotgun sequence.
ACCESSION   DASSVX010000300 DASSVX010000000
VERSION     DASSVX010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263643"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFO53_12925"
     CDS             <1..665
                     /locus_tag="VFO53_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877227.1"
                     /translation="TIKRTLMRGLSIPTGELITPQVYGYFPAANKRPAYDVNKAKALL
                     KEAGYPNGFEVTLDCPNDRYINDALICQALTSMWAKIGLKVNLDAMPKAQYFAKINKR
                     DTSLYMLGWAVATFDAQDAFLSLVHTPDGKGAGEYNDGGYSNPKMDALIDAMKSEADV
                     KKRLDMIHEALTLHTQDIAHLMLHQQIIPWAMRKNITVTHSADNRLRVWWVHVNDASA
                     RQ"
     gene            728..1714
                     /locus_tag="VFO53_12930"
     CDS             728..1714
                     /locus_tag="VFO53_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8877228.1"
                     /translation="MPAFILQRFVQAIVVMLVVALVAFSLFQYVGDPVQLMLGQFATP
                     DDRIRLTHDLGLDQPFYVQFYHFIVNAVHGQFGVSLRIGKPVSVLLAQRIPATLELSI
                     SAAILALVVGIPAGVYAGLRPNSWPARTALSLSLLGVSLPTFLIGILLILIFAVELGL
                     LPAFGRGEVVSLGGWTTGLLTPSGLRSLILPTITLGLFQLTLIMRLVRSEMLEVLRTD
                     YIKFARARGLSDRAVNFGHALKNTLVPVITITGLQLGSIIAFSIITEQVFQWPGMGLL
                     FIQAVSYVDIPVMAAYLCLIALVFVTINLIVDLLYYVVDPRLRIDRPGTAGH"
     gene            1725..2639
                     /locus_tag="VFO53_12935"
     CDS             1725..2639
                     /locus_tag="VFO53_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8877229.1"
                     /translation="MSAALRRSWDSDLAYSFRTSPYAIVAALVFLLCVGSSVFAPWVA
                     PHNPFDLGQLDLSNSLKPPAWLPGGEAVFPLGTDDQGRDILSTILFGARISLAVGVCA
                     VALSMVLGVALGLIAGYVGGKTDAFIMRVADVQLSFPAILVALLIDGVARALLPGAHE
                     SMAFYVLVLSIGVSGWVQYARTVRGSTFVEKNKEYVQAARLFGIHPLAIMARHVLPNV
                     LGPVLVIATIHIATAIITEATLSFLGVGVPPTQPSLGTLIRIGNDFLFSGEWWVTLFP
                     GAALVVMVLAINLLGDWLRDALNPRLRT"
     gene            2701..3678
                     /locus_tag="VFO53_12940"
     CDS             2701..3678
                     /locus_tag="VFO53_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8877230.1"
                     /translation="MPARALLDVRHLRVEFPTRRGVLTALDDVSFTIAPGEVLGVVGE
                     SGAGKSLTGASIIGLLEPPGRIAAGEVLLDGMRIDRLPQDQMRRIRGRRIGAIFQDPL
                     ASLDPLYSIGAQLTETIRTHLPMSEAAARERAVALLQEVGIPAAARRIDHFPHQFSGG
                     MRQRVVIALALAAKPQLIIADEPTTALDVSIQAQIITLLRRLCREHGTAVMLITHDMG
                     VIAETADRVAVMYAGRIVEIGPVADVIRAPKHPYTIGLMGSIPSIAGERERLVQIDGA
                     MPRLTAIPAGCPFHPRCRYVFDRCLHARPELLDAGSSRAACWLHAQAAQ"
     gene            3675..4796
                     /locus_tag="VFO53_12945"
     CDS             3675..4796
                     /locus_tag="VFO53_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET8877231.1"
                     /translation="MSREGGDHAGRALLQVSHATKIFDVSPPWLARVLGRRHRVLLHA
                     VDDVSFDIRRGETLGLVGESGCGKSTVARLIVGLYRLSSGAITYDGMDTAALSAGRAP
                     RTLRHKLQMIFQDPYASLNPRWRVTDIVAEPIRAQRLARGSPAIGARVDALLTKVGLS
                     AADGHKYPHQFSGGQRQRISIARALASEPEFLVCDEPTSALDVSVQAQVLNLMRDLQR
                     QLGLTYLFISHNLAVVHHVADRVGVMYLGRIVELADKAMLFASPQHPYTRMLLDAIPD
                     IAMSGKARTPVAGEVPNALSPPSGCTFHPRCPYANERCRVEVPALRPAAANGVVACHG
                     VEEGRIPLKVRDDLQGTEIHAVSETTLRIAGTDDAKSRL"
     gene            complement(4799..>5319)
                     /locus_tag="VFO53_12950"
     CDS             complement(4799..>5319)
                     /locus_tag="VFO53_12950"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET8877232.1"
                     /translation="GRLAAELNHDRLTGLPNRRFFCEWLSFAIANARRERAHVGVLLI
                     DIGGCAAVAELHGEPAAEALLVEVARRFRAAAREGDVFARLGASEFALATPNAHDARE
                     LALLAQRLRDTLNDPAQAPLADTPIGTSIGVAFFPENADDSAGVLAAANAAMFAARRA
                     GRNHVAFNALAA"
BASE COUNT          806 a         1833 c         1763 g          917 t
ORIGIN      
        1 agacgatcaa gcgcacgctg atgcgtggac tgtcgatccc gaccggcgag ctcatcacgc
       61 cgcaggtcta tggctacttc cccgcggcga acaagcgtcc agcgtacgac gtgaacaagg
      121 cgaaggcgct gctgaaggaa gccgggtatc ccaacggttt cgaggtgacg ctcgactgcc
      181 cgaacgaccg ctacatcaac gacgcgctga tctgccaggc gttgacgtcg atgtgggcga
      241 agatcggcct caaggtcaat ctcgacgcga tgccgaaggc gcagtacttc gccaagatca
      301 acaagcgcga caccagcctg tacatgctcg gctgggcggt cgcgacgttc gacgcgcagg
      361 acgctttcct gtcgctcgtg catacgcccg acggcaaggg cgccggcgag tacaacgacg
      421 gcggctacag caatccgaag atggacgcgc tgatcgacgc gatgaagagc gaggccgacg
      481 tcaagaagcg gctcgacatg attcacgagg cgctgacgct gcacacgcag gacatcgcgc
      541 acctgatgct gcaccagcag atcatcccgt gggcgatgcg caagaacatc accgtcacgc
      601 acagcgccga caaccggctg cgcgtgtggt gggtgcacgt caacgacgcg agcgcgcgtc
      661 aatgaacgac tgatccccct gccggagtgg cgcatgccgc tccggcatct gcttcgcaaa
      721 ccatccgatg cccgcgttca tcctgcagcg cttcgtgcag gcgatcgtcg tcatgctggt
      781 cgtcgcgctg gtggcgttct cgctgttcca gtatgtcggc gacccggtgc agctgatgct
      841 cggccagttc gcaacgcccg acgaccgcat tcgcctcacg cacgatctcg ggctcgacca
      901 gccgttctac gtccagttct accatttcat cgtcaacgcc gtgcacggcc agttcggcgt
      961 gtcgctgcgc atcggcaagc ccgtgtcggt gctcctcgcg cagcgcattc cggcgacgct
     1021 cgagctcagc atctcggcgg cgatcctcgc cctcgtcgtc ggcattccgg ccggcgtcta
     1081 cgcggggctg cgcccgaact cgtggccggc gcgaacggcg ctgtcgctgt cgctgctcgg
     1141 cgtgtcgctg ccgacgttcc tgatcggcat cctgctcatc ctgattttcg cggtcgagct
     1201 cggcctgctg cccgcattcg gtcgcggtga agtggtctcg ctcggcggct ggacgaccgg
     1261 gctcctgacg ccgagcgggc tgcgctcgct catcctgccg acgatcacgc tcgggctctt
     1321 ccagctgacg ctgatcatgc ggctcgtccg ctccgagatg ctcgaggtgc tgcgcaccga
     1381 ttacatcaag ttcgcgcggg cgcgcggcct gtccgatcgt gcagtcaact tcggccacgc
     1441 gctaaagaac acgctcgtcc cggtgatcac gatcaccggg ctgcagctcg gctcgatcat
     1501 cgcgttctcg atcatcaccg agcaggtgtt ccagtggccc ggcatgggac tgctgttcat
     1561 ccaggcggtg agctacgtcg acattcccgt gatggcggcg tacctgtgcc tgatcgcgct
     1621 cgtgttcgtg acgatcaacc tgatcgtcga cctcctgtac tacgtcgtcg atccgcgcct
     1681 tcggatcgac cggccgggca ccgcaggaca ttagcgtgac acgcgtgtcg gccgcgttgc
     1741 gtcgatcctg ggacagcgac ctcgcgtaca gcttccgcac ctcgccgtac gcgatcgtcg
     1801 ccgcactcgt gttcctgctg tgcgtcggga gctcggtctt tgcaccctgg gtcgcgccgc
     1861 acaatccgtt cgacctcggc cagctcgacc tgtcgaattc gctgaagcca cccgcgtggc
     1921 ttcccggcgg cgaggccgtg tttccgctcg gcaccgacga ccagggccgc gacatcctgt
     1981 cgacgatcct gttcggcgcg cgcatttcgc tcgccgtcgg cgtctgcgcg gtcgcgttgt
     2041 cgatggtgct cggcgtggca ctgggcctga tcgccggcta cgtcggtggc aagaccgacg
     2101 cgttcatcat gcgggtcgcc gacgtgcagc tctcgtttcc cgcgatcctc gtcgcgctgc
     2161 tgatcgacgg cgtcgcgcgg gcgctgctcc ccggcgcgca cgaaagcatg gccttctacg
     2221 tgctcgtgct gtcgatcggc gtatcgggtt gggtgcagta cgcacgcacg gtgcgcggct
     2281 cgacgttcgt cgagaagaac aaggagtacg tgcaggccgc gcggctcttc ggcattcatc
     2341 cgctcgcgat catggcccgg cacgtgctgc cgaacgtgct cggcccggtg ctggtcatcg
     2401 cgacgatcca cattgcgacg gcgatcatca ccgaggcgac gctgtcgttt ctcggcgtcg
     2461 gcgtgccgcc cacgcagccg tcgctcggca cgctgatccg catcggcaac gacttcctgt
     2521 tctccggcga atggtgggtg acgctcttcc cgggtgctgc actcgtcgtg atggtgctcg
     2581 cgatcaacct gctcggcgac tggctgcgcg acgcgttgaa tccgcggctg cgcacatgag
     2641 gcgggcgccg tgaatgccac gatcggcggc gccgcaatcg tgcgcgaggc gaacgcgatc
     2701 ctgccggccc gcgcgctgct cgacgtgcgg cacctgcgcg tcgagttccc gacccggcgc
     2761 ggcgtgctca ccgcgctcga cgacgtgtcg ttcacgatcg caccgggcga ggtgctcggc
     2821 gtcgtcggcg aatcgggtgc cgggaaatcg ctgacgggcg cgtcgatcat cggcctgctc
     2881 gagccgcccg gacgcatcgc ggcaggcgaa gtgctgctcg acggcatgcg catcgaccgc
     2941 ctgccgcagg accagatgcg acgtatccgc ggccggcgca tcggtgcgat cttccaggac
     3001 ccgctcgcgt cgctcgatcc gctgtactcg atcggcgcgc aattgacgga gacgatccgc
     3061 acgcacctgc cgatgagcga ggcggccgcc cgtgagcgcg ccgtggcgct cctgcaggag
     3121 gttggcatcc cggccgccgc gcgccgcatc gatcactttc cgcatcaatt ctcgggcggc
     3181 atgcgccagc gggtggtgat cgcgctcgcc cttgccgcaa agccgcagct catcatcgcc
     3241 gacgagccga cgaccgcgct cgacgtgtcg atccaggcgc agatcatcac gctgctgcgc
     3301 aggctctgcc gtgagcacgg caccgcggtg atgctgatca cgcacgacat gggggtgatc
     3361 gccgagacgg ccgaccgcgt cgcggtgatg tacgccggac gcatcgtcga gattggaccc
     3421 gtcgccgacg tgatccgcgc gccgaagcat ccctacacga tcggattgat gggatcgatc
     3481 ccgtcgatcg ccggcgagcg cgaacgcctc gtgcagatcg acggcgccat gccgcggctc
     3541 accgcgattc ccgccggctg cccgttccat ccgcgctgcc gctacgtgtt cgatcgctgc
     3601 ctgcatgcgc gtcccgaact gctcgatgcc ggttcgtcgc gtgcggcgtg ctggctgcat
     3661 gcgcaggcag cgcaatgagc cgcgaaggag gagaccatgc cgggcgcgcg ctcctgcagg
     3721 tgtcgcacgc gacgaagatc ttcgacgtgt cgccgccgtg gctcgcacgc gtgctcggcc
     3781 ggcggcaccg cgttctgctg catgccgtcg acgacgtgag cttcgacatc cggcgcggcg
     3841 aaacgctcgg cctcgtcggc gaatcaggct gcggcaagtc gacggtcgcg cggctgatcg
     3901 tcggccttta ccggttgtcg tcgggcgcga tcacgtacga cggcatggac accgccgcgc
     3961 tttcggcggg gcgcgcaccg cgaacgctgc gccacaagct gcagatgatt ttccaggacc
     4021 cgtatgcgag cctcaacccg cgctggcggg taacggacat cgtcgccgag ccgattcgcg
     4081 cgcagcggct tgcgcgcggg agcccggcga tcggtgcgcg cgtcgatgcc ttgttgacga
     4141 aggtcgggct gtcggccgcc gacggacaca agtatccgca ccagttctcg ggcggacagc
     4201 ggcagcgcat ttcgatcgcg cgggcgcttg cgagcgagcc cgagtttctc gtgtgcgacg
     4261 agccgacgtc cgcactcgac gtgtcggtcc aggcgcaggt gctgaacctg atgcgcgacc
     4321 tgcagcgcca gctcggcctc acttacctgt tcatttcgca caatctcgcc gtcgtgcacc
     4381 acgttgccga tcgggtcggc gtcatgtatc tcggccgcat cgtcgagctt gccgacaagg
     4441 cgatgctgtt cgcgtcgccg cagcacccct acacgcgaat gctgctcgat gcgatccccg
     4501 acatcgcgat gagcggcaag gcgcgcacgc cggtggcggg cgaagtgccc aatgcgctct
     4561 cgccgccgtc gggctgcacg ttccacccgc gatgcccgta tgcgaacgag cgctgccgcg
     4621 tcgaggtgcc ggcgctgcgg cctgcagcgg cgaatggcgt ggttgcgtgt cacggcgtcg
     4681 aggaggggcg gattccgctc aaggtccgag acgaccttca gggcaccgaa atccacgcag
     4741 tgtcagagac gacgttgcgc atcgccggta ccgacgacgc gaaaagtcgt ctctgacgct
     4801 aagcggcgag cgcgttgaac gccacgtgat tgcggccggc gcggcgcgcg gcgaacatcg
     4861 ctgcattcgc ggccgccagc acgccggccg aatcgtcggc gttttcggga aagaacgcga
     4921 cgccgatcga cgtgccgatc ggcgtgtcgg caagcggcgc ttgcgccgga tcgttcagtg
     4981 tgtcgcgcag gcgctgcgca agcaatgcga gctcgcgcgc atcgtgcgca ttcggcgtcg
     5041 cgagcgcgaa ctcgctcgcg ccgagacgcg cgaacacatc gccttcgcgt gccgcggcac
     5101 gaaagcggcg cgcgacctcg acgagcagtg cctcggctgc cggttcgccg tgcaattcgg
     5161 cgacggccgc acagccgccg atgtcgatga gcagcacgcc gacgtgcgcg cgctcccgcc
     5221 gcgcgttcgc gatcgcaaac gaaagccatt cgcagaagaa gcgccggttc ggcagcccgg
     5281 tgagccgatc gtggttgagc tccgcggcaa gccggccat
//
LOCUS       DASSVX010000301         4870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_463585, whole genome shotgun sequence.
ACCESSION   DASSVX010000301 DASSVX010000000
VERSION     DASSVX010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463585"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /locus_tag="VFO53_12955"
     CDS             <1..1941
                     /locus_tag="VFO53_12955"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HET8877233.1"
                     /translation="EALVAVDVNSARATKGGDIETTAFNTNVEAADEIARQLRLRDLG
                     GLIVIDFIDMESAKNQREVENRLRDALKYDRARVQLGKISRFGLMELSRQRLRPALAE
                     SAYLPCPRCHGIGHIRGTESTALHILRIIQEEAMKDNTAQIVAQMPVDVATFLLNEKR
                     AEVLTVETRFKVNVLLVPNRHLETPNYSVQRMRHDELNQGEPLPASFQMVEQPEDMDA
                     FARKEEAKEPRQEAVVKGITPSQPAPVSAPRAPARAEAPLEAPHANSWLSKVLHWFRT
                     PPEAATTPAQVPAKPAAAERVRDSRPPRPREGRGRGDARRDGHPHRDERPRGERRDAR
                     ASDESRRAEAQGAIEMPARSQAAPRKDGERRERREPREGKGSATPRPPRDNQRQEARE
                     ATQAQSPGRKQSARAELTDVLENANAPATAAPVGESIRPAMPGNGAEESREGGSRRRR
                     GRRGRGGDRAERAERAPRAQRVASSDESAAGEPAAAIDQRTDIAGTHEPAPVTSPLAT
                     IVAPSQDTESEPARSLESDATEPLSSAPSAQVAEPVTEDEPRRQAASTPIPEAAVSGH
                     AQAAPMASAAMQSPDEVDVPTAALRLPPESGLVLVETRFSAPAIDEQEQAQPRPRRAR
                     PPRATLADEPLQMVETHKRDNA"
     gene            complement(1982..2437)
                     /locus_tag="VFO53_12960"
     CDS             complement(1982..2437)
                     /locus_tag="VFO53_12960"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HET8877234.1"
                     /translation="MGNICRSPTAESVFREHAQRAGLLDELRIESAGIGDWHVGQAPD
                     NRAITHARRRGYDLARLRARQVTREDFAQFDFILAMDLRNLRDLKALCPPDYKGRLGL
                     FLDFAPHLGLREVPDPYYSGADGFETVLELTERASEGLLAHIRRLRYSG"
     gene            complement(2497..3276)
                     /locus_tag="VFO53_12965"
     CDS             complement(2497..3276)
                     /locus_tag="VFO53_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HET8877235.1"
                     /translation="MEMNDQVAIVTGAASGIGRAIARRFARAGARVVLADLDRGAAAA
                     TAREIDADPRRAVGIAMDVADERAVDSGVAEVISQFGRIDVLVSNAGIQIVHPLEEFS
                     YAEWKKMLAIHLDGAFLTTRACLPHMYRAGRGSLIYMGSVHSKLASVLKAPYVTAKHG
                     LIGLAKTMAKEGAAHGVRANVICPGFVRTPLVERQIPEQSRTLGISEEDVIRKVMLKD
                     TVDGEFTTLDDVADVALFFAAFPSNALTGQSLVVSHGWYMQ"
     gene            3429..>4870
                     /locus_tag="VFO53_12970"
     CDS             3429..>4870
                     /locus_tag="VFO53_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877236.1"
                     /translation="MALHRRHHFGLFALLLLACAAGMSDAADTHKVLRVASSDITSLD
                     PQQGTDLYSTRVASAIFEALYEFEYLSAGSKVIPNTAQAMPVVTDDGKTWTIRMKQGI
                     RFADDPVFKGKPRELIADDYVYSIKRTLDPNLRSGGDPALTDLIVGARPIVDAARKPG
                     GRFDYDAPIDGLRATDRHTLVLRLAHPDYTMLERLAGLPMMAVAREVVEAAGSDIMRR
                     PVGTGPYRLREWRPASKVVLEANPDYRKVVFPDNADAPQQELVRSMRGKTLPQIGRIE
                     ISIIEESQPEILAFTQANLDYMALSGDDTRRVMENGKLRPDLVQRGVRHLRFGSPSVT
                     FTYFNMDDPAVGGYSTQQIALRRAIGMGFDVDEMIRVLFAGNALPANQLLPPGVSGHD
                     PQLPPKSLYDPAGARSLLDRFGFKDRDGDGYREAPDGKPLTIMRGTLPESWYREADTL
                     WKKNMDAIGIRMQVQQQTFPELLNLSRAGKL"
BASE COUNT          890 a         1652 c         1611 g          717 t
ORIGIN      
        1 gaggcgctcg tcgccgtcga tgtcaattcg gcccgcgcga ccaagggtgg cgacatcgaa
       61 acgacggcgt tcaacaccaa cgtcgaagcg gccgacgaaa ttgcccgcca gcttcggctg
      121 cgcgacctcg gcggcctgat cgtgatcgac ttcatcgaca tggagagcgc caagaaccag
      181 cgcgaagtcg aaaaccggct gcgcgatgcg ctcaagtacg atcgcgcacg tgtacagctc
      241 ggcaagatct cgcgcttcgg cctgatggag ttgtcgcgtc agcggctgcg tcctgcgctg
      301 gccgagtcgg cgtacctccc ctgcccgcgc tgccacggca tcggccacat ccgggggact
      361 gaatcgacgg cactgcacat cctgcgcatc attcaggaag aggcgatgaa ggacaacacg
      421 gcgcagatcg ttgcgcagat gccggtcgac gtcgcgacgt tcctgctcaa cgaaaagcgt
      481 gccgaggtgc tgaccgtcga gactcgcttc aaggtcaacg tgctgctcgt tccgaaccgt
      541 cacctcgaaa cgccgaacta cagcgtgcag cggatgcgcc acgacgagct caaccagggc
      601 gagccgttgc ccgcatcgtt ccagatggtc gaacagcccg aggatatgga cgccttcgcg
      661 cgcaaggaag aagcgaagga gcctcgtcag gaagcggtcg tcaagggcat caccccatcg
      721 cagccggcac cggtcagcgc gccgcgcgcg ccggcacgtg ccgaggcgcc gctcgaggcg
      781 ccacacgcaa attcgtggct gtcgaaggtg ctgcactggt ttcgcacgcc cccggaggcc
      841 gccacgacgc ccgcgcaggt gccggcaaag ccggcggccg ccgagcgtgt gcgagacagc
      901 cgtccgccgc gaccccgcga aggacgggga cgaggcgacg cgcgtcgcga cgggcatcca
      961 catcgcgacg agcgaccacg cggtgagcgt cgcgatgcac gtgcaagcga cgaatcgcgt
     1021 cgcgccgagg cgcaaggggc catagaaatg cccgcgcgtt cgcaagcggc gccgcgcaaa
     1081 gatggtgagc gacgcgagcg ccgggaaccg cgcgaaggga aaggcagcgc cacgccacgc
     1141 ccgccgcgcg ataaccagcg tcaggaagcc cgcgaagcga cgcaagcgca gtcgcccggg
     1201 cggaagcagt cagcgcgcgc cgagctcacc gatgtgctgg aaaacgcgaa cgccccggca
     1261 acagccgcgc cggtcggcga atcgatccgt ccggccatgc caggcaatgg cgccgaggag
     1321 tctcgcgagg gcggctcgcg ccgccgtcgc gggcgccgcg gtcgtggtgg tgatcgagca
     1381 gagcgtgccg agcgagcgcc ccgcgcgcag cgcgtcgcat ccagcgacga atccgcggcg
     1441 ggcgagccgg cggcggccat cgatcaacgc accgacattg ccggcaccca tgaaccggcg
     1501 ccggtgacgt cgccgctggc cacgatcgtg gcgccatcgc aggacacgga aagcgagccg
     1561 gcgcgttcac tcgagtcaga tgcaaccgag ccgttgtcga gcgcaccgtc cgcgcaagtg
     1621 gccgagccgg tgaccgagga cgaaccgcgg cggcaggctg catcgacgcc gatcccggag
     1681 gccgccgtgt cggggcatgc tcaggcggcg ccgatggcat cggcggccat gcaatcaccg
     1741 gatgaagtcg atgttccgac ggcagccctt cgcctgccac ccgaatcggg tctcgtgctc
     1801 gtcgaaaccc gcttttcggc gcctgcaatc gacgagcagg agcaagcgca accgcgaccg
     1861 cgacgcgcac gtccaccgcg cgcgacgctt gccgacgagc cgctccagat ggtcgaaacg
     1921 cacaagcgcg acaacgcctg agacctcgtt ggcgaaggga cggacgcgtc cgtcccttcg
     1981 ctcaaccgct gtaacgcagg cggcgaatgt gggcgaggag cccctcggac gcccgttcgg
     2041 tcaactcgag caccgtctcg aatccatcgg cgccgctgta atagggatcg ggcacttcgc
     2101 gcaatccaag gtgcggcgcg aagtccagga agagcccgag acggcctttg tagtcgggcg
     2161 ggcaaagcgc cttcaggtcg cgcagattgc gaagatccat cgcgaggatg aaatcgaact
     2221 gcgcgaagtc ttcacgcgtc acctggcgtg cacgcaacct tgcaaggtcg tagccgcggc
     2281 ggcgtgcatg ggtgatcgcc cggttgtcgg gcgcctgccc gacgtgccag tcgccgatgc
     2341 ctgccgactc gatgcgcaat tcgtcgagaa ggcccgcgcg ctgcgcgtgc tcgcgaaaga
     2401 cgctctccgc cgttggtgag cggcagatgt tgcccatgca cacgaacagc accgacgcgc
     2461 gcacgttcct tgcgggcgcg aggtcgccgg cgcgcctcac tgcatgtacc agccgtggct
     2521 cacgaccagc gattggcccg taagtgcgtt ggacggaaat gccgcgaaga acagcgccac
     2581 gtcggcgacg tcgtcgagcg tcgtgaactc gccgtcgacg gtgtccttca gcatgacctt
     2641 gcggatcacg tcttcctcgg aaatgcccag cgtgcgcgac tgctcgggaa tctgacgctc
     2701 gacgagcggc gtacggacga atcccgggca gatcacgttg gcgcgcacgc cgtgcgcagc
     2761 gccctccttc gccatcgtct tcgcgaggcc gatgaggccg tgcttcgccg tcacataggg
     2821 cgccttcagc accgaggcga gcttcgagtg caccgagccc atgtagatga gacttccgcg
     2881 tcccgcccgg tacatgtgcg gcagacacgc gcgcgtggtc agaaacgccc cgtcgaggtg
     2941 gatcgcgagc atcttcttcc attccgcata gctgaattct tcgagcgggt ggacgatctg
     3001 gatgccggcg ttgctcacca gcacatcgat gcggccgaac tgcgagatca cctcggccac
     3061 cccgctgtcc acagcgcgct cgtcggcaac atccatcgcg atgccgaccg cgcgccgcgg
     3121 atcggcgtcg atctcgcgcg cagttgccgc ggctgcaccg cgatcgagat cggccagcac
     3181 gacgcgcgcg ccggcgcgtg cgaagcggcg cgcaatcgcg cggccgatgc cgctcgcggc
     3241 accggtgacg atggccacct ggtcattcat ttccataggg actcccacat ggcgatttcg
     3301 aacccgtccg acggcgtgcg atgattcgca tgctgcttcg cacgcaggcc atcgcgtggc
     3361 accgcaacgg cctgcactgc gcgaagtata ggcgcccgcc ccgctccctg ctcccgcgag
     3421 gttcgaccat ggcattgcat cgaaggcacc atttcggcct cttcgcgctg ctattgctcg
     3481 cctgtgcggc gggcatgtcc gacgcggccg acacgcacaa ggttctgcgc gtcgcctcga
     3541 gcgacatcac gagtctcgat ccgcagcagg gcaccgacct gtactccacg cgcgtcgcgt
     3601 ccgcgatctt cgaggcgctc tacgaattcg aatatctgtc ggcgggctcg aaggtgatcc
     3661 cgaacaccgc acaggcgatg ccggtcgtca cggacgacgg aaagacatgg acgatccgga
     3721 tgaagcaggg catccggttc gccgacgatc ccgtattcaa gggcaagccg cgcgagctca
     3781 tcgccgacga ctacgtgtac tcgatcaaga ggacgctcga tcccaatctg cgcagtggcg
     3841 gcgatccggc gctgaccgat ctcatcgtcg gcgcgcgtcc gatcgtcgat gcagcgcgca
     3901 agcccggcgg caggttcgat tacgacgcgc ccatcgacgg ccttcgcgcc accgatcggc
     3961 atacgctggt gctgcgcctt gcgcatcctg actacacgat gctcgagcgc cttgccggcc
     4021 tgccgatgat ggcggtcgcg cgcgaggtgg tggaagcagc cggcagcgac atcatgcgcc
     4081 ggccagtcgg caccgggccc tatcggttgc gcgaatggcg gcccgcgtcg aaggtcgtgc
     4141 tggaagcgaa ccccgactac cgcaaggtcg tgtttcctga caacgcggat gcgccgcagc
     4201 aggagctcgt gcgcagcatg cgaggaaaaa cgctgccgca gatcgggcgc atcgaaatca
     4261 gcatcatcga ggagtcgcag ccggagatcc tcgccttcac ccaggcaaac ctggattaca
     4321 tggcgctgag cggcgacgac acgcggcgcg tgatggaaaa cggcaagctg cggccggatc
     4381 tggtgcagcg cggcgtacgc catcttcgct tcggctcacc gagcgtcacg ttcacgtatt
     4441 tcaacatgga cgacccggcc gtcggcgggt attcgacgca gcagatcgca ttgcgacgcg
     4501 cgatcggcat gggcttcgat gtcgacgaga tgatcagggt gctgttcgcg ggcaacgcgc
     4561 ttccggcgaa ccagctcctg cctcccggcg tcagcggtca cgatccgcag ctgcccccga
     4621 agtcgctgta cgatccggcg ggtgcgcgta gcctgctcga ccggttcggc ttcaaggatc
     4681 gcgacggcga cggttatcgt gaagcgcccg acggcaagcc gctcacgatc atgcgtggca
     4741 cgcttcccga atcgtggtac cgcgaagcgg acacgctgtg gaagaagaac atggacgcga
     4801 tcggcattcg gatgcaggtc cagcagcaga cgtttcccga gcttctcaac ctgagtcgcg
     4861 ccggcaagct
//
LOCUS       DASSVX010000302         7569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1247148, whole genome shotgun sequence.
ACCESSION   DASSVX010000302 DASSVX010000000
VERSION     DASSVX010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247148"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VFO53_12975"
     CDS             complement(<1..1086)
                     /locus_tag="VFO53_12975"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET8877237.1"
                     /translation="MDNGHSKIRHYRRTAWRSTKAPSIRWAALPRSLTEERMAYLATR
                     GSTRIGRAMALALVFIVPVIGVGIARAAPPENSQAGGKVHVVEARVKPILRIGQLEFR
                     DLNGNGQLDVYEDWRKPVEQRVADLVSRMTLEEKAGMMQITSFSAATAGGFINDRHIV
                     HLILRDGVSGRDLALRNDQFQQIAEGTRLGIPLVFAANPRDHISDALVFEQVDATGQW
                     PGTLGLAATNDLKLIRAFADTIRGQWVAQGIRKMYGYQVDVASEPRWNRVQTTYGEGP
                     WWSAAITREIVLGFQGQHLGPDSVAETIKHFPGDGAVLNGLDPHFAWGQYAVYPTAGS
                     LFQYQLPPFQAAVDAGTSAIMSYYNSQN"
     gene            1134..2099
                     /locus_tag="VFO53_12980"
     CDS             1134..2099
                     /locus_tag="VFO53_12980"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET8877238.1"
                     /translation="MDLRQLRYFMAVAEERNFGRAAARLHISQPPITRQIHMLESELG
                     VLLFERTRWGVNLTPAGEELLASASQIEALVDYASDRARRAGRGAIGRLDVGVFGSGA
                     ISIVPTILRRYLNANPAVDVIVLNVPQPAQLVALRQRRILITFDRYLPDDADLTVETV
                     AHEGLVLALKDTHALAKLPVISIATLAKEPMVMARDVRHRVWVTDLCRAHGFEPKLSQ
                     LAGDMISGIVMAANGFGVEIVPESAQALKLPGLAYRPLESSARGYKAMELQCAYLRND
                     ASPLLPGFLDVVRDYRNKRTARTSRALSAIPAVKRPSRRAVDAVR"
     gene            2225..4693
                     /locus_tag="VFO53_12985"
     CDS             2225..4693
                     /locus_tag="VFO53_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET8877239.1"
                     /translation="MPEDATKSAANPLPIIAALAALAAGATLLQFCTRLPPAPGALTI
                     IACALVVAAVVALRAQADRRVAVATLLRFAIAVVTCAALGFCYAAWRADWRLADALPT
                     TWEDADLRITGVVDDLPQNDDTGSRFAFAVERALTSGAVVPHRVSLAWHAPRATRDAP
                     VATPPVVHAGERWTLTVRLKRPHGNVNAHGFDLEAWLLEHDLRATGYVRNDAGNVRIT
                     AFAGRAGDHVQRARERVRERVGNALPDAPYRGVLVALAIGDQRAIPESQWAVFNKTGI
                     THLVSISGLHVTVFAALAAACALALARRWPALTLRVPARKVAALVGAAVAFGYVLLAG
                     AEVPAVRTLLMLVVAAFGLWLGRPGTAALVWLWSLVAVLCWDPWAGLAPGFWLSFGAV
                     GLLLYAGSNRLRIAPARSFRERLRESLLESARAQWVVTIGLVPGTLALFQQVSVVSVL
                     ANAVAIPVVTLAVVPLALLGIVAPIDLPWIVAHAALSVLMRYLEWLAALPLAAWTSHA
                     PRGWTVAVAVVGILWLIAPRGVPGRAFGALWTLPLAFVLPPGVTEAGVRVVVLDVGQG
                     LAVLVTTAHHTLLYDTGPRFSDSVDAGGRIVVPFLRAAGVARLDALIVSHADSDHSGG
                     ALSILHAVPVDRVVSSLPADHPIVAYRARGPPATHCVAGMQWTWDGVAFEIVHPLLSA
                     YTEGARKSNDLSCVLRIVSGGGSVLLTGDIEAASEAEILARDAAIAADVLVVPHHGSR
                     TSSTPAFVAAVSPHFAVIAAGYRNRFGHPRGEILARYAHAGAARPRTDHGGAITVALN
                     PGQPIEVFAERERARRYWYDLPAD"
     gene            complement(4700..5662)
                     /locus_tag="VFO53_12990"
     CDS             complement(4700..5662)
                     /locus_tag="VFO53_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HET8877240.1"
                     /translation="MSGANDAAPRIDVAIPDISRWATGNAGIPYAWCFETPVAGPRVT
                     IQALTHGNEVCGATALDWLLRKRIRPVRGTLTLIFANAAAYESFDANEPYAARCIDED
                     FNRIWDASVLDGARRSRELARARELRPVYDATDFLLDLHSMTDACPALALAGRHRKGV
                     ELARALGMPQYIVVDAGHRAGKRLRDYAGFDDPGDARNALLVECGQHWERHAATVAQQ
                     CALRFLRHFGVIDAELAASLIGTDELPPQRVIEVTDVVTIASAAFSFALPVEGMMTIG
                     HAGTLLARDGEREIRTPYDDCVLIMPARRSKRGETGVRMGRYVD"
     gene            complement(5670..6887)
                     /gene="argE"
                     /locus_tag="VFO53_12995"
     CDS             complement(5670..6887)
                     /gene="argE"
                     /locus_tag="VFO53_12995"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HET8877241.1"
                     /translation="MNAPGHAGTLDTRPVSPADLVGYDMIRRLVAFPTVSRDSNLALI
                     EWVRRYLDDLGAATTLTFDDERRKANLFATLAARDGNTTQRGIVLSGHTDVVPVDGQP
                     WDTNPFEATLVGDRLYGRGVADMKSFSAIGLAFVPEFVRRGLARPLHFALSYDEEIGC
                     IGVRRLIADVVAKEIAPTGCIIGEPTGMQLVVANKGRKTWRCRVRGHEAHSSLTPQGV
                     NAVQIACELVAWITERGRELRAGPRQDPAYDVPFTTTHVGVIEGGTALNIVPRDCAFE
                     LEIRHLPMDDPDALFERLRRHADSLLPAMKAVDRETYIEFDALSGMPGFDNAGGNPMV
                     EIGHACSGSALEHKVSFGTEAALFHAASMPAVICGPGHIEQAHQPNEWVSLDQLARCE
                     AFMRRLAEQVCVA"
     gene            7055..>7569
                     /locus_tag="VFO53_13000"
     CDS             7055..>7569
                     /locus_tag="VFO53_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HET8877242.1"
                     /translation="MHALLVSLFTLVTVFAVPPVCAQPLPLDRIRLPPGFTIELVARV
                     PGAREMAFGARGTLFVGSVEGSVYALALDDAKTGAAKVRRIATGLRQPVGVAFRDGAL
                     YVSAVSRILRLPDIEAHLDHPPAPVVVSDRFPTDGSHGWKFIAFGPDGKLYVPVGAPC
                     NVCEPDRDRYA"
BASE COUNT         1094 a         2599 c         2546 g         1330 t
ORIGIN      
        1 gttctggctg ttgtagtacg acatgatcgc gctcgttccc gcgtcgaccg ccgcctggaa
       61 cggcggaagc tggtactgga acaggcttcc tgcggtcgga tagaccgcgt actggcccca
      121 cgcgaagtgc gggtcgagac cattcagcac cgcaccgtcg ccgggaaagt gcttgatggt
      181 ctcggccacc gaatccgggc ccagatgctg gccctggaat cccaggacga tctcgcgcgt
      241 gatcgcagca ctccaccagg ggccttcacc gtaagtcgtc tgcacacgat tccaccgggg
      301 ctcggaggcg acgtcgacct gatagccgta catcttgcgg atgccctgcg cgacccactg
      361 cccgcggatg gtgtcggcga acgcccggat cagcttgagg tcgttggtcg ccgccaatcc
      421 cagcgtcccg ggccactgac ccgtggcatc gacctgctcg aagaccagcg catcgctgat
      481 gtggtcgcgc ggattggcag cgaacaccag aggaatgccg agccgcgtgc cttccgcgat
      541 ctgctggaac tggtcgttgc gcaacgcgag gtcccgcccg gaaacgccat cgcgcagaat
      601 cagatgcacg atgtggcggt cgttgatgaa gccgcccgcc gtcgccgcgc tgaacgacgt
      661 gatctgcatc atcccggcct tctcctcgag cgtcatgcgc gagacgaggt cggcaacacg
      721 ctgttcgacc ggcttgcgcc agtcttcgta cacgtcgagc tggccgttgc cgttcagatc
      781 gcggaactcg agttgcccga tcctgaggat cggcttgacg cgtgcctcga cgacgtggac
      841 cttgcccccg gcctgggaat tttctggtgg cgccgctcgg gcaataccga cgccgatgac
      901 cggtacgatg aagaccaggg cgagcgccat tgctcgacca atccgggtgc tgccccgggt
      961 tgccagatat gccatgcgtt cctccgtgag tgaccgcggc agcgcggccc atcggattga
     1021 cggcgccttc gtgcttcgcc acgctgtgcg ccggtaatgt cggattttcg aatgcccgtt
     1081 gtccaatacc ttttgccgtg tgcgcgatac cctttcggta tggagaaacg cctgtggatc
     1141 tgcgtcagtt gaggtacttc atggccgttg cagaagagcg caatttcgga cgcgctgcgg
     1201 cgcggcttca catatcgcag ccaccgatca ctcggcagat tcatatgctc gagtccgaat
     1261 tgggcgtcct gcttttcgag cgcacgcgct ggggcgtgaa tctcaccccc gccggcgagg
     1321 aactgctggc gagcgcaagc cagatcgaag cgctcgtcga ctacgcatcg gaccgcgcgc
     1381 gccgcgcagg acgtggggcc atcggacgcc tcgatgtcgg cgtattcggc tcaggcgcga
     1441 tcagcatcgt cccgaccatc cttcgtcgct atttgaacgc gaatcccgcg gtcgacgtca
     1501 tcgtgctcaa cgtgccccag ccggcgcaac tggtggcgtt gcggcagagg cggatactga
     1561 tcacgttcga ccgctatctt ccggacgacg ccgatctgac cgtcgaaacg gttgcgcacg
     1621 agggtttggt gctggcgctg aaggacaccc acgcgctggc gaagctgccg gtcatatcga
     1681 tcgccactct cgccaaagaa ccgatggtca tggcgcgcga cgtgcgccac cgggtgtggg
     1741 tgacggatct ctgccgcgcg cacggattcg agccgaagtt gagccagttg gcgggcgaca
     1801 tgatctccgg gatcgtcatg gcggcgaacg gattcggggt cgagatcgtt ccggagtccg
     1861 cacaggcgct caagctgccc gggctcgcct atcgtccgct cgaatccagc gcgcgtggct
     1921 acaaggccat ggagcttcag tgcgcgtacc tcaggaacga tgcatcgccg ctgctaccgg
     1981 gttttctgga cgtcgtgcgc gactaccgca acaagcgaac ggcaaggacg tcgcgcgcgc
     2041 tgtctgccat cccggcggtc aagcgtccat cacgccgcgc cgtcgatgcc gttcgctgac
     2101 gaacctcgcg ccgttcgata gtcgcagtcc cggcccgcgg atcgagcgtc ttcgcgcatc
     2161 ggccgaacgg cctatcgtcc tcgaaagcga cgcgtgatga gatgcggctt catccgcgct
     2221 ccgcgtgccc gaggacgcaa cgaagtccgc cgctaatccg ctcccgatca tcgccgcgct
     2281 ggcggcgctc gcggccggcg caacgctgtt gcagttctgc acgcgcctgc ctcctgcgcc
     2341 cggcgccctc acgatcatcg cgtgtgcgct cgtcgtggct gcggttgttg cgcttcgcgc
     2401 acaggccgac cgccgggtcg ccgtcgccac gctgctgcgc tttgcgatcg ccgtcgtcac
     2461 ctgtgcagcg ctcgggttct gctacgccgc ctggcgcgcc gactggcggc tcgccgacgc
     2521 cctgccgacg acgtgggagg acgccgactt gcgcatcacc ggcgtcgtcg acgacctgcc
     2581 gcagaacgac gatacgggct cgcgtttcgc gtttgcggtc gagcgcgcgc tgacctccgg
     2641 cgcggtcgtt ccacatcgtg tctcgctcgc ctggcacgcg ccccgtgcga cgcgcgacgc
     2701 acccgtcgca acgccgccgg tcgtgcatgc cggcgagcgc tggacgctga ccgtgcgtct
     2761 caagcgtccg catggcaacg tcaatgcgca tggcttcgac ctcgaggcgt ggctgctcga
     2821 gcacgatctg cgcgccactg gctatgtccg caacgacgca gggaacgtgc gcatcacggc
     2881 gttcgcgggc cgcgccggcg atcacgtgca gcgggcccgc gagcgcgtgc gcgagcgggt
     2941 cggcaacgca ttgcccgatg cgccgtatcg cggcgtgctc gtggcgctcg ccatcggcga
     3001 tcagcgcgcc attcccgaat cgcaatgggc ggtgttcaac aagacgggca tcacgcacct
     3061 cgtgtcgatc tcgggcctgc acgtcaccgt gttcgcggcg ctggccgccg cgtgcgcgct
     3121 ggcgcttgca cggcgatggc cggcgctcac gctgcgagtg ccggcgcgga aggtggcggc
     3181 actcgtcggt gccgccgtcg cgttcggcta tgtgctgctc gccggtgcgg aggtgccggc
     3241 ggtgcgcacg ttgctgatgc tcgtcgtcgc ggcgttcggg ttatggcttg ggcgtcccgg
     3301 cacggccgcg ctggtctggc tctggtcgct cgtcgccgtg ttgtgctggg atccctgggc
     3361 cggcctggca cccggcttct ggctgtcgtt cggcgcggtg ggactgctgc tctacgccgg
     3421 aagcaatcgc ctgcgcatcg ccccggcgcg ctcctttcgc gagcgcctgc gcgaatcgtt
     3481 gcttgaatcg gcacgcgcgc agtgggtggt cacgatcggt ctcgtgccgg gcacgctcgc
     3541 gctgttccag caggtgtcgg tcgtgtcggt gcttgcgaac gcggtcgcca ttcccgtcgt
     3601 cacgctcgca gtcgtcccgc tggcgttgct cggcatcgtc gcgccgatcg acctgccgtg
     3661 gatcgttgcc catgccgcgc tttcggtgtt gatgcgctat ctcgaatggc tcgcggcgct
     3721 tccgctggct gcatggacgt cgcatgcgcc gcgcggctgg acggtggccg tcgccgtggt
     3781 gggcattctc tggctgattg cgccgcgtgg tgtgccgggc cgcgcgttcg gtgcgctatg
     3841 gacgctgccg ctggcgttcg tgctcccgcc tggcgtcacc gaggcaggcg tgcgcgttgt
     3901 cgtgctcgac gtggggcagg gactcgcggt gctggtgacc accgcgcatc acacgcttct
     3961 ctacgacacc ggcccgcgct tcagcgacag cgtcgatgcc ggcgggcgca tcgtcgtgcc
     4021 cttcctccgc gccgcggggg tcgcgcggct cgacgcgctg atcgtgagtc acgccgacag
     4081 cgaccattcc ggcggcgcgc tgtcgatcct ccatgccgta ccggtggacc gggttgtctc
     4141 gtcgctgccg gccgatcatc cgatcgtcgc gtatcgcgca cgcgggccgc cggcgacgca
     4201 ctgcgtggcc gggatgcaat ggacgtggga cggcgtcgca ttcgaaatcg tgcatccgct
     4261 gctttccgcg tataccgagg gcgcacgcaa gagcaacgac ctgtcgtgcg tgctgcggat
     4321 cgtatcgggc ggcgggagcg tcctgctgac gggcgatatc gaggcggcgt cggaggccga
     4381 aatcctcgcg cgcgacgctg caatcgccgc cgatgtgctc gtcgttccgc accacggctc
     4441 gcgcacgtcg tcgacacccg cgttcgttgc cgcggtgtcg ccgcacttcg cggtgatcgc
     4501 ggcgggctat cgaaaccggt tcgggcatcc gcgcggcgaa atcctcgcgc gttatgcgca
     4561 cgccggcgcg gctcgcccgc gcaccgacca cggcggcgcg atcaccgtgg cgctgaatcc
     4621 cggccagccc atcgaggtgt ttgccgagcg cgagcgcgcc cggcgctatt ggtacgacct
     4681 gccggcggat tgattcggct cagtcgacgt aacggcccat gcgcaccccg gtctcgcccc
     4741 gcttcgaccg gcgcgccggc atgatcagca cgcaatcgtc gtacggcgtt cgaatttcgc
     4801 gctcgccgtc gcgcgcgagc agcgtgcccg cgtgcccgat cgtcatcatg ccctcgaccg
     4861 gcagcgcgaa cgagaacgca gcgctcgcga tggtcaccac atcggtgacc tcgatgacgc
     4921 gctgaggcgg caattcgtcc gtaccgatca gcgaggccgc gagctcggcg tcgatgacgc
     4981 caaaatggcg cagaaagcgc agcgcgcatt gctgcgcgac ggtagctgcg tggcgttccc
     5041 aatgctgtcc gcattcgacc agcagcgcgt tgcgcgcatc gccgggatca tcgaagcccg
     5101 cgtagtcgcg cagccgcttg ccggcgcgat gaccggcgtc gacgacgata tattgcggca
     5161 tgccaagcgc gcgcgcgagc tcgacccctt tgcggtgacg gcccgccaac gcaagtgccg
     5221 gacacgcatc ggtcatcgag tgcagatcga gcaggaaatc ggttgcgtcg tacaccggcc
     5281 gcagctcgcg ggcgcgagcg agctcgcgcg agcggcgcgc gccgtcgagc accgacgcgt
     5341 cccagatccg gttgaagtcc tcgtcgatgc agcgcgccgc gtagggctcg ttcgcatcga
     5401 acgattcgta cgcggcggcg ttggcgaaga tcagcgtcag cgttccgcgg accggacgta
     5461 tgcgcttgcg cagcagccag tcgagcgccg tcgccccgca cacttcgttg ccgtgcgtca
     5521 gcgcctggat cgtgacgcgc ggtccggcga ccggcgtctc gaagcaccac gcgtacggga
     5581 tgcccgcgtt gcccgttgcc cagcgcgaga tatcgggaat cgcgacgtcg atccggggcg
     5641 ccgcgtcgtt cgcgccgctc atcgacgcat catgcgacgc agacctgctc cgcgagcctg
     5701 cgcatgaacg cctcgcaacg cgcaagctgg tcgaggctca cccactcgtt gggctgatgc
     5761 gcctgctcga tgtggccggg gccgcagatc accgccggca tcgacgcggc gtggaagagc
     5821 gccgcctcgg tgccgaacga gaccttgtgc tcgagcgccg acccgctgca cgcatgtccg
     5881 atctcgacca tcggattgcc gccggcgttg tcgaagccgg gcatcccgga aagtgcgtcg
     5941 aactcgatgt acgtttcgcg atcgacggcc ttcatcgccg gcagcagcga atcggcgtgg
     6001 cggcgcagcc gctcgaacag cgcatcggga tcgtccatcg gcaggtggcg tatctcgagc
     6061 tcgaacgcgc agtcacgcgg aacgatgttg agcgcggtgc ctccctcgat cacaccgacg
     6121 tgcgtcgtcg tgaacggcac gtcgtacgcc ggatcctgtc gcggcccggc gcgcagttcg
     6181 cggccgcgct cggtgatcca cgcaacgagc tcgcaggcga tctgcacggc attcacgccc
     6241 tgcggcgtca acgacgagtg cgcttcgtga ccgcgcacac ggcagcgcca ggtcttgcgc
     6301 cccttgttcg cgacgacgag ttgcattccg gtgggctcgc cgatgatgca gccggtcggc
     6361 gctatctcct tcgcgacgac atcggcgatc agccggcgta cgccgatgca tccgatctcc
     6421 tcgtcgtacg acagcgcgaa atgcagcggc cgcgcgaggc cgcggcggac gaactcgggg
     6481 acgaacgcaa gcccgatcgc cgagaagctt ttcatgtcgg cgacgccgcg tccgtacagc
     6541 cggtcgccga cgagcgtcgc ttcgaacgga ttggtatccc atggctggcc gtcgacgggc
     6601 accacatcgg tatgccccga gagcacgatc ccgcgttgcg tcgtgttgcc gtcgcgcgcc
     6661 gccagcgtgg cgaacaggtt cgccttgcgt cgctcgtcgt cgaaggtgag ggtggttgcc
     6721 gcgcccagat cgtcgagata gcgacgcacc cattcgatca gcgcgagatt ggagtcgcgg
     6781 ctcaccgtgg ggaacgcgac gagccggcga atcatgtcgt agccgacgag gtccgcgggg
     6841 ctcaccgggc gcgtatcgag cgtgcctgcg tgtccgggcg cgttcatggt cgttccacga
     6901 ttgcaaacat gcagcatagc accgcgtcga atcgacgatc cgttcaacgg tcgccgctgc
     6961 gccactcgaa tagacgagcg ggccgcgcga attgaaccga tgtgcacgcc tccgcatcca
     7021 agctcgatcg cggcgccgca aagggatcga ccggatgcat gcgctcctgg tttcgttgtt
     7081 cacgctcgtc accgtcttcg cggtgccgcc ggtttgcgcg caaccgctcc cgctcgatcg
     7141 cattcgcctg ccgccgggct tcacgatcga gctcgtggcc cgcgtccccg gtgcgcgcga
     7201 aatggcgttc ggcgcgcgcg gcacgctgtt cgtcggctcg gtcgaaggca gcgtgtatgc
     7261 gcttgcgctc gacgatgcaa aaacgggcgc ggcgaaggtg cgcaggattg ccacggggct
     7321 tcggcagccg gtcggcgtcg cattccgcga cggcgcgctg tatgtctcgg cggtgagccg
     7381 catcctgcgc ctgcccgaca tcgaggcgca cctcgatcat ccgcccgcgc cggtcgtcgt
     7441 ctccgatcga ttcccgaccg acgggtctca cggctggaaa ttcatcgcct tcggccccga
     7501 cggcaagctc tacgtgccgg tcggcgcgcc gtgcaacgtg tgcgagcccg accgcgaccg
     7561 ctacgcgaa
//
LOCUS       DASSVX010000303         4832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1363615, whole genome shotgun sequence.
ACCESSION   DASSVX010000303 DASSVX010000000
VERSION     DASSVX010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363615"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFO53_13005"
     CDS             complement(<1..290)
                     /locus_tag="VFO53_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET8877243.1"
                     /translation="MNRLLPLAGLLLLSWAGAAAAQSAEQIPMRDFFRNPDRAYYRIS
                     GDGKTLSFMQPWERRMNVFIQPVGSSQDPVRITSEKDRDITDYFWKGANRVV"
     gene            complement(373..825)
                     /locus_tag="VFO53_13010"
     CDS             complement(373..825)
                     /locus_tag="VFO53_13010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8877244.1"
                     /translation="MTSATRAGAARLLGAFLGDDAHYRASAAAYGDGGAGALARALDL
                     FLARPELGFVWLAAAGDEIVGACVICYAISTSRGGIVAKLDDVTIDRDWQGRGVGTAM
                     LDALRAELRRLGVGRIDTACHRANEGAWRFYARHGFRPLDEERIALLL"
     gene            complement(860..1324)
                     /locus_tag="VFO53_13015"
     CDS             complement(860..1324)
                     /locus_tag="VFO53_13015"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET8877245.1"
                     /translation="MSAALAPSTRDAYTAFLAIPTRWMDNDTYGHVNNVTYYSYFDTV
                     VNEHLIRAGGLDIREGNSIGIVVETACRFHKPLSFPETIDAGLAVAHIGRSSVVYEIA
                     LFRQRDDEAAATGRFVHVWVDRAERRPIDVPAPIRTAVEALRRSPAHASHRD"
     gene            complement(1321..2580)
                     /gene="glcF"
                     /locus_tag="VFO53_13020"
     CDS             complement(1321..2580)
                     /gene="glcF"
                     /locus_tag="VFO53_13020"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HET8877246.1"
                     /translation="METNLLPSLLETPEGREADAILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQALEGGEVTAKTQLHLDRCLTCRSCETTCPSGVRYGRLVDIGRHHV
                     DARVGRAPVDAAKRSALRRGLLSKSLFGAALTLGRIAKPFLPRAIGDSIPAQHAAGAW
                     PAPRHQRRVVIALGCVQPSIAPNIDAATARVLDRLGISAIAVEGGRCCGALSYHLSEH
                     DEARALARRNIDAWWPHVERGAEAIVVTASGCGVMVRDYGAVFADDALYAEKARRIAA
                     LLRDPAEIISAAWSGVDERVAATNTRRTLAFHPPCSLQHGLKVRGVVEPLLEAIGFTL
                     VPVTDAHLCCGSAGTYSILQPELADALKVRKLQALDESHPDVIATANIGCITHLQSGT
                     DTPIRHWIELVDAAMSESAASAGGGRR"
     gene            complement(2586..3674)
                     /gene="glcE"
                     /locus_tag="VFO53_13025"
     CDS             complement(2586..3674)
                     /gene="glcE"
                     /locus_tag="VFO53_13025"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HET8877247.1"
                     /translation="MTSGTADPARADIIDRVRAAAADELPLRVRAGGTKDFYGETLAG
                     DIVDVRSLNGIVAYAPAELVITARAGTALDEIEAALAEHGQMLAFEPPHHGVAATLGG
                     IVACGFSGPRRAQAGAVRDFVLGVRIIDGTGEPLAFGGQVIKNVAGFDVSRLMTGALG
                     TLGVLTEISLKTLPRPRRETTRVIECDGASALRRVNEWGGKPLPVSATCYIEGRLWVR
                     LSGAAAGITAATRDIGGDEVDGTLFWPSIRDQTHARFATSMRNDATLWRLSVRSTAPW
                     LEIAGEQIVEWGGALRWVVTTDGAAGSTLRAWAATNGGHAALYRGNDKSAGAFQPLAP
                     PLLALHRRLKDVFDPHRILNRGRLFAEI"
     gene            complement(3671..4366)
                     /locus_tag="VFO53_13030"
     CDS             complement(3671..4366)
                     /locus_tag="VFO53_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET8877248.1"
                     /translation="MFKLYIGNRNYSSWSLRGFLLAKLSGEPFDEVDVPLTGLTPNPA
                     NLAFSPTGLVPCLHEGEIVVWDTMAIAGYLAERHPQMWPDDVAARAWARSISAEMHAG
                     FGALRNEMAMCIRERVDVRPWSSALAANIKRIIGIFDESRRRFGAGGAFLCGRFSIAD
                     CFYGPVAFRFRTYGVDAPGAAGKYVEALLAHPFMQQWERAALAETSIIEADEPRLLYR
                     DKVAAAGGASRST"
     gene            complement(4381..>4832)
                     /locus_tag="VFO53_13035"
     CDS             complement(4381..>4832)
                     /locus_tag="VFO53_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET8877249.1"
                     /translation="EERSRLWKARHNAYYAALALGPGKQGFATDACVPISRLTDCVLE
                     TKADVAASGLVAPIVGHVGDGNFHLVILFDPTDADERSRAEALAARVSLRAISMGGTC
                     TGEHGIGVHKLDALRAEHGDAIALMQAVKRALDPLNLLNPGKTVPLT"
BASE COUNT          863 a         1623 c         1576 g          770 t
ORIGIN      
        1 tagacgacgc ggttcgcgcc tttccagaaa taatcggtga tgtcgcgatc cttctcggac
       61 gtgatgcgaa ccggatcctg cgagctgccg acgggctgga tgaagacgtt catccgccgc
      121 tcccatggct gcatgaacga cagcgttttg ccgtcgcccg agatccggta gtacgcgcga
      181 tcgggattgc ggaagaagtc ccgcatcgga atctgttcgg cggactgtgc cgcggcggct
      241 ccagcccagc tgagcagcag caggccagcc aacggaagca atcggttcat ttattcctca
      301 gcagaattgg gcgatgcagt tggatgcggc cgttggccgg agtggattca accaccttca
      361 cgaactgcga gcctacagta aaagcgcgat ccgctcctcg tcgagcggcc ggaagccgtg
      421 ccgtgcgtag aacctccacg cgccttcgtt ggcgcggtgg cacgcggtgt cgatgcggcc
      481 gacgcccagg cgacgcagct ccgctcgcag cgcgtcgagc attgccgtgc cgacgccacg
      541 accctgccag tctcgatcga tcgtcacatc gtcgagcttc gccacgatgc cgccacgcga
      601 tgtcgaaatc gcatagcaga tgacgcacgc gccgacgatc tcgtcgccag ccgccgcgag
      661 ccacacgaag cccagctccg gccgcgcgag aaagaggtcg agtgctcgcg cgagcgcacc
      721 tgcgccgcca tcgccgtacg cagccgcgga agcgcggtag tgcgcatcgt caccgagaaa
      781 agcgcccagc aaccgcgcag cacccgcgcg ggttgccgag gtcatcggcg cgcaaaccac
      841 cgtcgcgcgc gagccttgtt caatcccgat gggatgcatg tgccggcgaa cgccgcagcg
      901 cctcgaccgc ggtgcggatt ggtgcgggaa catcgatggg acgacgctcg gcgcgatcga
      961 cccacacatg aacgaaccgc ccggtggcgg cggcttcgtc gtcccgctgg cgaaaaagcg
     1021 cgatttcgta gacgacggag gagcgtccga tgtgcgcgac agccagtccg gcgtcgatcg
     1081 tttccggaaa cgacagcggc ttgtgaaagc ggcacgcggt ttcgacgacg atgccgatcg
     1141 aattaccttc gcggatgtcg agcccgccgg cgcgaatgag gtgctcgttc accacggtat
     1201 cgaaatatga gtagtacgtc acgttgttga cgtggccata cgtgtcgttg tccatccagc
     1261 gcgtcgggat tgccaggaac gcggtgtacg catcgcgcgt cgaaggcgcc agcgccgcgc
     1321 tcatcgccgg ccacccccgg cgctcgcggc agactccgac attgctgcgt cgacgagctc
     1381 gatccaatgc cggatcggcg tgtccgttcc cgactgcaga tgcgtgatgc acccaatgtt
     1441 tgccgtcgct atcacatcgg gatgcgattc atcgagcgcc tgcaacttgc gcacctttaa
     1501 tgcatcggcg agctcgggtt gcagaatcga atacgtgccg gccgagccgc aacaaaggtg
     1561 tgcgtcagtg accgggacga gcgtgaagcc gatcgcttcg agcagcggct cgaccacgcc
     1621 gcgaaccttg agcccgtgct gcagcgagca cggcgggtga aaggcgagtg tgcgccgcgt
     1681 gttcgtcgcg gcaacgcgct cgtcgactcc actccacgcg gcgctgatga tctccgcagg
     1741 gtcgcgcaga agcgccgcga tgcgccgtgc tttctccgcg tagagcgcat cgtccgcgaa
     1801 cacggcacca taatcgcgga ccatgacgcc gcagccgctg gccgtgacga cgatcgcttc
     1861 ggcaccgcgt tcgacatgcg gccaccaggc gtcgatgttt cgccgcgcaa gggcccgcgc
     1921 ttcgtcgtgc tcggacaggt gatacgacaa agcgccgcag cagcgtccgc cttcgacggc
     1981 aatcgctgaa atgccgagtc gatcgagcac gcgagccgtc gccgcatcga tgttcggcgc
     2041 aattgagggc tgcacgcaac cgagtgcgat gacgacgcgt cgttgatggc gcggtgccgg
     2101 ccatgcgccc gccgcgtgct gcgcgggaat cgaatcgccg atcgcgcgtg gcaggaatgg
     2161 cttcgcgatg cggccgagcg tcaatgcggc gccaaacagc gacttcgaca ggagcccgcg
     2221 ccgcagcgcc gagcgcttcg ccgcgtcgac gggcgcgcgg cccacacgcg catcgacgtg
     2281 atgtcgaccg atgtcgacga gccggccata gcggacgccg gaaggacacg tcgtttcaca
     2341 gcttcggcac gtcagacagc gatcgagatg gagctgcgtc ttcgccgtca cttcaccgcc
     2401 ttcgagcgcc tgcttgatca ggtagatccg cccgcgcggc ccgtcgagct cgtcaccgag
     2461 aagctgataa gtcgggcagg tcgccgtgca gaagccgcaa tgcacacagg cgcgcaggat
     2521 tgcgtcggcc tcgcgacctt ccggcgtctc cagcagcgac ggcagtagat tcgtttccat
     2581 tgctgctaga tctcggcaaa gagtcggccg cgattgagga tgcggtgcgg gtcgaacacg
     2641 tccttcagcc gccgatgcaa cgcgagcagc ggcggcgcga gcggttgaaa ggcgccagcg
     2701 gacttgtcgt ttccacgata gagcgctgcg tgaccaccgt tcgtcgccgc ccacgcgcgc
     2761 agcgtcgatc cggcagcacc atcggtcgtg acgacccagc gcaatgcacc gccccactcg
     2821 acgatctgct caccggcgat ctcgagccat ggcgcggtcg agcgcaccga cagccgccac
     2881 agcgtcgcgt cgttgcgcat cgatgtcgca aagcgcgcat gcgtctggtc gcgaatcgac
     2941 ggccagaaca gcgtcccgtc gacttcgtcg ccaccgatgt cccgcgtagc ggcggtaatt
     3001 ccggcggctg cgcccgacaa acgcacccac aggcgcccct cgatatagca ggtcgccgac
     3061 acgggcaacg gcttgccgcc ccattcgttg acgcgtcgaa gcgcgctggc gccgtcgcat
     3121 tcgatcacgc gcgtcgtctc gcggcgcggc cgaggcagtg ttttcaacga gatttccgtc
     3181 agcacgccga gcgtgccgag cgcaccggtc atgagcctcg atacgtcgaa cccggcgacg
     3241 ttcttgatca cctggccgcc gaatgcgagc ggctcaccgg tgccatcgat gatgcgtacg
     3301 ccgagcacga agtcgcgaac ggcaccggcc tgcgcgcggc gcggccccga gaatccgcat
     3361 gcgacgatgc cgccgagtgt cgcggcgacg ccgtgatgcg ggggctcgaa cgccagcatc
     3421 tggccatgct cggcgagcgc cgcctcgatc tcatcgaggg ccgtgccggc acgcgccgtg
     3481 atgacgagct cggcgggtgc ataggcgacg attccattca gcgagcgtac gtcgacgatg
     3541 tcgccggcga gcgtctcgcc gtagaaatcc ttggttccac ccgcgcgaac gcgcagcggc
     3601 agctcgtcag ccgctgccgc gcgaacgcgg tcgatgatat cggcgcgcgc cggatcggca
     3661 gttccggacg tcacgtcgac cgcgacgctc cgccggcagc ggcgaccttg tcgcgataca
     3721 aaagccgcgg ctcatcggct tcgatgatcg acgtttcggc gagtgcggca cgttcccatt
     3781 gctgcatgaa cggatgcgca aggagtgcct ctacatattt gcccgcggca cccggggcgt
     3841 cgaccccgta cgtccggaag cgaaacgcca cggggccgta gaagcagtcg gcgatcgaga
     3901 atcgtccgca caggaacgcg ccgcccgcgc cgaagcgacg gcgcgattcg tcgaagatgc
     3961 cgatgatgcg cttgatgttc gcggcgagtg cgctcgacca cggacgcacg tcgacgcgct
     4021 cgcgaatgca catcgccatc tcgttgcgca gcgcgccaaa gcccgcatgc atctcggcgc
     4081 tgatcgatcg cgcccacgcg cgggccgcaa cgtcgtcggg ccacatttgc ggatgccgct
     4141 cggcgaggta ccccgcaatc gccatcgtgt cccacacgac gatctcgcct tcgtgcaggc
     4201 acggcacgag gcccgtcggc gaaaacgcga ggttggccgg attcggtgtc agccccgtca
     4261 atggaacgtc gacttcgtcg aaaggctcgc ccgagagttt ggcgagcaaa aagccgcgta
     4321 acgaccacga cgagtaattg cggttgccga tatagagctt gaacatgaac gtgcgagtag
     4381 tcaggtcaac ggaacggtct tgcctgggtt gagcaaattg agcggatcga gcgcgcgctt
     4441 caccgcctgc atcagcgcaa tcgcatcgcc gtgctcggcg cggagcgcgt cgagcttgtg
     4501 cacgccgatg ccgtgctcgc ccgtgcacgt gccgcccatg ctgatcgcgc gcaaagacac
     4561 gcgtgccgca agtgcctccg cgcggctgcg ctcatctgcg tccgtcgggt cgaacaggat
     4621 gacgagatgg aaattgccat cgccgacgtg tccgacgatc ggcgcgacca gtccgctcgc
     4681 cgcaacgtcg gctttggtct ccagcacgca gtcggtcagt cgcgagatcg gcacgcacgc
     4741 atcggtggca aagccctgct tgccgggccc aagcgccagc gcggcatagt acgcgttgtg
     4801 acgcgccttc cagagccgcg agcgctcctc gg
//
LOCUS       DASSVX010000304        12869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1563919, whole genome shotgun sequence.
ACCESSION   DASSVX010000304 DASSVX010000000
VERSION     DASSVX010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12869
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563919"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFO53_13040"
     CDS             <1..254
                     /locus_tag="VFO53_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877250.1"
                     /translation="QPQKFPGWTYESGNVFYIALPDTTTGACASGTQPIWRFFNQRTT
                     NHRYTADHELRDEMRADPTTWIPEGYGPDAVIMCAPMGS"
     gene            complement(313..996)
                     /locus_tag="VFO53_13045"
     CDS             complement(313..996)
                     /locus_tag="VFO53_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET8877251.1"
                     /translation="MPHPLELEFMTRTWRCIFIAATAMMSGACIAQEAAQPAAAAPDP
                     AKAKQIATQVCAACHGADGNSAAAANPNLAGQGADYITLQLMHFKAGVRVNATMQAMA
                     ASLSDDDMRGLGAYFSQQKPKGLAAKDAALVKTGRQLWRGGDSATDVPACAGCHSPTG
                     AGIPKNYPRLAGQYADYVYAQLKAFKAGARGADPGGKDVNGRIMATIAGNMTDEQMKA
                     IADYAMGLR"
     gene            1108..1713
                     /gene="yihA"
                     /locus_tag="VFO53_13050"
     CDS             1108..1713
                     /gene="yihA"
                     /locus_tag="VFO53_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HET8877252.1"
                     /translation="MPGPIEGASFVTGAAEAAQLPAEGLPEIAFAGRSNAGKSSAINA
                     LARQTRLAHASRTPGRTRQINFFRLRSGALVTDLPGYGYAAVPHALKRAWQDFLWSYV
                     TSRSTLVGLVLIVDARHGLKALDVDLLSAFLPSSRPVLILATKIDKLNVAERRRAVTD
                     IQAALLRAFAERAGAVSVIGFSATSRQGVELADRAIERWLG"
     gene            1883..2902
                     /gene="hemB"
                     /locus_tag="VFO53_13055"
     CDS             1883..2902
                     /gene="hemB"
                     /locus_tag="VFO53_13055"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET8877253.1"
                     /translation="MSFSYSGAFPARRPRRMRRDDFSRRLARETRLSADDFIYPVFVH
                     GEPRATPVTSLPGVARKSIDLLLVDAEHCVAAGVPAIALFPVIPAERKSEGAAEAWND
                     EGLVPRAVRALKARFPQLGVVTDIALDPYTSHGQDGLVDDTGYVMNDETVEALVRQAL
                     CHAAAGVDIVAPSDMMDGRVAALRSALDAKRFIHTRILAYSAKYASSFYGPFRDAVGS
                     ARSLAHGDKHTYQMDPGNSDEALWETYLDIQEGADMVMVKPGLPYLDIVRRVKDEFGM
                     PTAVYQVSGEYAMLKAAAQAGWLDLRACVLEALTGMKRAGADAILTYYALEAAAWLKN
                     GDPDR"
     gene            complement(2899..3501)
                     /locus_tag="VFO53_13060"
     CDS             complement(2899..3501)
                     /locus_tag="VFO53_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877254.1"
                     /translation="MHRPRRDREPQGRDAQNRARIADLAARLIAEHGLADWSLAKRKA
                     ARQLMLPERTTLPGDDEIEIALAAHHELFDGDEHTATLRAQREEALVWLRFFAAFEPV
                     LAGGVAAGWATEHSDIRIELVADDSKLVELALINAGIAYSVAPGVAQHAAPELLIATP
                     QGDVRAIVRTPFAARQRPRRDETRLDEARLAALLAGEDRS"
     gene            complement(3503..5830)
                     /locus_tag="VFO53_13065"
     CDS             complement(3503..5830)
                     /locus_tag="VFO53_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HET8877255.1"
                     /translation="MLKRWLVYTASVVLGLAVVGGLLGAFVLALLWPTLPPLDALTDY
                     QPKVPLRVVSADGDLLGEFGEERRAVVRIAEVPDVMKHAILAAEDERFYQHGGVDYLS
                     VLRAALANATSGTQQGAGTITMQVARNFFLTREKTVSRKLREALLAWKIEASLSKDEI
                     LELYINQIFLGQRAYGFAAAAQTYFGKPLSEITVAEAATLAGLPKAPSAYNPITNPRR
                     AKTRQLYVLRRMHELHYINDDQFREAQNAPLKLRPATREVSGVHAEFVAEMARQVVFD
                     AYGDSAYTRGITVWTTIHRADQEAAYAAVRKGVIDHDQRHGYRGPEGYVSLPKDPAEQ
                     DAALDRVFQENPDSDNIVTGVVLQATSSQVRAVLANGDQAEVTGAGLKFAQRALGDKA
                     PANQKIRTGAIIRLTRDDKGHYAITQMPLAEAAFVAVRPDDGAILALIGGFDYERNKF
                     NHATQALRQPGSAFKPFIYSAALEKGFTPATIINDAPFFVPAEKAGGEAWEPKNYEGK
                     FEGPMRLRVALAHSKNLVTIRVLQAIGPQYAQDYIARFGFDPKLHPAYLTMGLGAGAA
                     TPMQMATAYSVFANGGYRITPYLISRITDSHGDPLSEAKPAMAGKDAERAIDPRNAFI
                     MQSLLREVITSGTATRALSLKRKDIAGKTGTTNDDVDAWFCGFNVAQVGIAWIGYDQP
                     KTLGTNETGSVAALPMWIAYMEKALKGVPETLRDPPSGVVSLRINPETGLRDDTSNLS
                     DWFMAEFTPRMAQDALAPALMPGSAPARDVRNQLF"
     gene            5979..7070
                     /locus_tag="VFO53_13070"
     CDS             5979..7070
                     /locus_tag="VFO53_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HET8877256.1"
                     /translation="MLADNFSSALDLFKTRSPTLIGVDIASTSLKLVELSAAGKGTYR
                     LDRYVIEPLPKDVVADGNVLNIELCADVLKRAWKRLGSRNRNVALALPAAMVITKKII
                     VAAGQREEDLELTVEAEANQYIPFALDEVNLDFQEIGPAPNNPDEVEVLIAASRKEKV
                     EDRVAVAEAAGLKARVMDVESYSTQEAFQLIAPSLPANGRDQNIALVDIGAHVMHFYV
                     LRNGQMLFSRDQAFGGNQLTQDIQRAFNLSADEAESARRNQGLPENYDHDVLQPFMES
                     LALEVTRALQFFFTSTSYNQVDQIVIAGGCALLPGLDELVAKRAGVATLVANPFATMQ
                     TSDRVRPRQLAQEAPMLMIATGLAMRGFE"
     gene            7086..7706
                     /locus_tag="VFO53_13075"
     CDS             7086..7706
                     /locus_tag="VFO53_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HET8877257.1"
                     /translation="MRINLLPHREQKRQARERQFVSLAITLAILGVAIVALVHVILAA
                     RIDNQISRNQLLKTKIAELDAQIKEIDRLRDQIQQVLARKQVVETLQSNRNEAVHLLD
                     QLVRQLPEGVYLSSVKQTDKKVTIVGFAQSNARVSTLMRNIEGSPWLEKPELVEIRLV
                     SVPAQGRTGPQNLNQFTLNFQIRRATPEAVAAPGAPGKAQAKGGKA"
     gene            7703..8329
                     /locus_tag="VFO53_13080"
     CDS             7703..8329
                     /locus_tag="VFO53_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HET8877258.1"
                     /translation="MTLDDFRRLNFREVGKWPLAPKIIVLALVVLFIVALGAYFDWKD
                     QWDSLQTAQQEEGRLRDQFKEKKAKAINYDLYVQQLAEVEQSFGALVKQLPNRSEIDA
                     LLTDINQAGLGRGLQFDLFRPAPQERMADFYAELPISIKITGNYHDIGAFASDVAALP
                     RIVTLNDLAIVNDKGTLTMDAVAKTFRYLDEEEMAKQRALAKAAKGKK"
     gene            8326..8865
                     /locus_tag="VFO53_13085"
     CDS             8326..8865
                     /locus_tag="VFO53_13085"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HET8877259.1"
                     /translation="MKRGAFIAIASAALVAGCGASSHKDLTDWMAEQGKGVRGHLDPI
                     PQIKPYEPFVYNDFDLPDPFKPRKIEPMKSESTSKLAPDLNRRREPLEAYPIESLSMV
                     GTLEKGKTRYALVKTPERDIYQVRQGMHMGQNFGVIVDIDDADIKLKELVQDGSGDWS
                     ERSSTLNLQQADQKQEQKK"
     gene            8913..11111
                     /gene="pilQ"
                     /locus_tag="VFO53_13090"
     CDS             8913..11111
                     /gene="pilQ"
                     /locus_tag="VFO53_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HET8877260.1"
                     /translation="MRAWLAGIAAFVVAGAALAQAPNQIEQVSVTRAASGNTIVRFEL
                     KSPPANPPAGFATASPPRIALDFFDTTSALPSNQRNVDDPALRTLQFVEAGKRTRVVF
                     SLNKPQSFDTKVEGNAVVVTLSDSTSAVAQAAPAPVQRFAEGAPGDQQHALRDVDFRR
                     GRNGEARIIVDLSDNNTGIDIRQQGRQLIVDFQKTSVPRNLERRLDVADFNTPVVTVD
                     TFEQNGNARMVIQPQGLWEHSAYQAENRFIIEVKPVVDDPNKLVQGSRAGYKGEKLSL
                     NFQNIEVRSVLQVIADFTGLNIVTSDTVTGSLTLRLKDVPWDQALDIILQTKGLDMRK
                     NGNIVLIAPREELALKEKQQLESQAQINELEPLQTETFQLNYAKANDILAIINGRSSA
                     GGGAPGAAAVAQFLSKRGTAFVDPRTNILFVTDIASRLEEVRRVIRQIDTSVRQVLIE
                     ARIVIADDTFSRQLGIRFGQQTGATLFGRRYSLGSGGSLATQPVVSLSGGAGGTVTRT
                     TETQTPFELASGIATPGYSNDPQLNINLPVANPAGQLALTLINLGSGNLINLELSALE
                     ADNRGKVVSSPRVITGDNQKAHIEQGTEIPYVTPGSANSPATVSFKKAVLALDVTPQI
                     TPDGRVIMQVEIRKDSVGQLVNVQGGGQVPAIDTKNVLTQIAVNNGDTAVIGGIYEEV
                     IRNDKTQVPFLGDVPVLGNLFKTTTRSSQKTELLIFLTPRIVKDTVTSVR"
     gene            11177..11707
                     /locus_tag="VFO53_13095"
     CDS             11177..11707
                     /locus_tag="VFO53_13095"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET8877261.1"
                     /translation="MSLHRGNLFLVGLMGAGKTTLGRQLARRLDKPFVDADHELEARL
                     GVRIPTIFEVEGEAGFRDREEAVIDELSRRSGIVLATGGGAVMREQSRVRLRENGTVL
                     YLHAEPETLWQRLRNSRQRPMLHAADPRNRLVELYQLRDPLYREVADHVIESDREAVI
                     RFVRWLDAEPCNGSPG"
     gene            11727..12812
                     /gene="aroB"
                     /locus_tag="VFO53_13100"
     CDS             11727..12812
                     /gene="aroB"
                     /locus_tag="VFO53_13100"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET8877262.1"
                     /translation="MKTLTVGLGARTYPIHVGNALLSRAASLLDVQRGQRGVIVTNAI
                     VAAHHLAPLRTSLGGAGMHVDVVLLPDGEAHKNQKTLDDLLTRLLELRIERSTTLIAL
                     GGGVVGDIAGFAAAIYQRGIAFVQVPTTLLAQVDSSVGGKTGINHPLGKNMIGAFWQP
                     RAVLIDTAVLSTLPDRELSAGLAEVIKYGAIRDAEFFAWLETNMAALLARNDAALERA
                     IVRSCEIKAQIVAADEREAGERALLNFGHTFGHAIEAAQGYGEWLHGEAVAAGMVCAA
                     RLSGRVCGLSADEATRLANLVVAAKLPAAPPRIARERWLELMQRDKKVQSGAMRFVLL
                     ERLGRAVVRGDVAENDVVQSVKPDAMR"
BASE COUNT         2253 a         4256 c         4290 g         2070 t
ORIGIN      
        1 accagccgca gaagtttccg ggatggacgt acgaaagcgg caacgtgttc tacattgcgc
       61 tgcccgatac gacgacgggt gcgtgcgcga gcggaacgca gccgatctgg cgcttcttca
      121 accagcgcac gaccaaccat cgctacacgg cggatcacga actgcgcgac gagatgcgcg
      181 ccgatccgac gacgtggata cccgaaggct acgggcccga cgccgtgatc atgtgcgcac
      241 cgatgggcag ttgacgaaag ggtcggagac gagtttcgca acgcgaaagt cgtctctgac
      301 cctacttcgg ttttagcgca ggcccatggc gtagtcggcg atggccttca tctgctcgtc
      361 ggtcatgttg cccgcgatcg tcgccatgat gcggccgttg acgtccttgc cacccggatc
      421 ggcgcctcgc gcgccggcct tgaaggcctt gagctgcgcg tacacgtagt cggcgtactg
      481 gcccgcgagg cgcggatagt tcttcgggat gcccgcgccg gtcggcgaat ggcatccggc
      541 gcaggcggga acgtcggtgg ccgaatcgcc gccccgccac agctgccggc cggtcttcac
      601 gagcgccgca tccttcgccg cgagcccctt cggcttctgc tgcgaaaagt aggcgccgag
      661 cccgcgcatg tcatcgtcgg aaagcgatgc cgccatcgcc tgcatcgtcg cgttcacgcg
      721 cacgccggcc ttgaaatgca tcaattgcag ggtgatatag tcggccccct gtcccgcgag
      781 gttcggattc gccgcggccg cgctgttgcc gtccgcgccg tgacacgccg cgcacacctg
      841 ggtggcgatc tgcttcgcct tcgcaggatc gggcgccgcc gctgccggtt gcgccgcctc
      901 ttgtgcgatg caagcgccgg acatcatggc ggtggcggca atgaagatac agcgccaggt
      961 acgggtcatg aactcgagct ccaggggatg cggcattcgt caccggcgcg cgccgtgcag
     1021 cgtgcgatga cgaagcgtgt tgcgatgaaa aaacgcgcca ttgtagcgga ctcgcagcac
     1081 ccggccaaaa tcgcggatgc accgcacgtg ccgggaccga tcgaaggtgc ctcgttcgtg
     1141 accggtgctg ccgaggccgc gcagctcccg gcggaagggc tgcccgagat tgcgttcgcc
     1201 ggccgttcga acgcgggcaa gtcgagcgcc atcaatgcgc tcgcacgcca gacgcggctg
     1261 gcccatgcga gccgcacgcc cggacgaaca cgccagatca acttctttcg cctgcgaagc
     1321 ggcgcgctcg tcaccgacct tccagggtat gggtacgccg ccgtgccgca tgcgctgaag
     1381 cgcgcatggc aggatttcct gtggtcgtac gtgacgtcgc gctcgacact cgtcggtctc
     1441 gtgctgatcg tcgatgcgcg gcatggactg aaagcgctcg acgtcgatct cctgtcggcg
     1501 ttcctgcctt cgtcgcggcc ggtgctgatc ctcgcgacga agatcgacaa gctgaacgtc
     1561 gccgagcggc gtcgcgcggt caccgatatt caagcggcac tcctgcgtgc gttcgccgaa
     1621 cgtgccgggg ccgtgtcggt gatcgggttc tcggcgacgt cacgccaggg tgtcgagctg
     1681 gccgaccggg cgatcgagcg gtggcttgga tagaaaaagg ccccgcgatc aaggggagtg
     1741 acgcggggcc tcaaaacgcc ctgccggtac gaaaccggcg agggcacccg ctcagggagg
     1801 aaagcgggag acgttgaccg tccgctttct gtgaccgtca tcgaaaatgc gagttccatc
     1861 aacgacagca aaaggtgcat cgatgagttt cagctatagc ggagcgtttc ccgcgaggcg
     1921 gccgcggcgc atgcgccgcg acgatttctc gcgccggctc gcgcgcgaaa cgcgactgtc
     1981 cgccgacgat ttcatctacc cggtgttcgt tcacggcgag ccgcgcgcca caccggtgac
     2041 atcgctcccc ggtgtcgcgc gcaagtcgat cgacctcctg ctcgtcgatg ccgaacactg
     2101 cgtcgccgcg ggcgtgccgg cgatcgcgct cttcccggtg atccccgccg agcgcaaatc
     2161 ggagggcgcc gccgaagcgt ggaacgacga gggcctcgta ccgcgcgcgg tacgcgcgct
     2221 gaaggcgcgg ttcccgcagc ttggcgtggt caccgatatc gccctcgatc cgtacacgag
     2281 ccacggacag gacggcctcg tcgacgacac cgggtacgtg atgaacgacg agacggtgga
     2341 ggcgctcgtg cgccaggcgc tttgccacgc ggcggccggt gtcgacatcg tcgcgccgtc
     2401 ggacatgatg gacggccgcg tcgcggcgct gcgcagcgca ctcgacgcga agcgcttcat
     2461 ccatacgcgc atcctcgcct actcggcgaa atacgcatcg tcgttctacg gaccgttccg
     2521 tgatgcggtg ggatcggcaa ggagcctcgc gcacggcgac aagcacacgt accagatgga
     2581 ccccggcaac agcgacgaag cgctgtggga gacttacctc gacatccagg aaggcgccga
     2641 catggtgatg gtcaagccgg gcctgcccta tctcgatatc gtccggcgcg tgaaggacga
     2701 attcggcatg cccaccgccg tgtaccaggt gtcgggcgaa tacgcgatgc tgaaggccgc
     2761 cgcgcaggcc ggatggctcg acctgcgcgc ctgcgtgctc gaggcgctga cggggatgaa
     2821 gcgcgccggc gccgacgcaa ttctcaccta ttacgcgctc gaggccgccg cgtggctcaa
     2881 gaacggggac ccggaccgct aggatcgatc ctcgcccgcc agcaacgccg cgagccttgc
     2941 ctcgtcgagc cgcgtttcgt cgcggcgcgg tcgctgccgt gcggcaaacg gcgtgcgcac
     3001 gatcgcgcgc acgtcgcctt gcggcgtggc gatgagaagc tcgggcgccg catgctgcgc
     3061 aacgccgggg gcgacgctgt aggcgatgcc ggcgttgatg agcgcgagct cgacgagctt
     3121 cgaatcgtcg gcgacgagct cgatgcggat gtcgctgtgc tcggtggccc agccggcggc
     3181 gacgccaccg gcgagcaccg gctcgaatgc ggcaaagaag cgcagccaca cgagcgcttc
     3241 ctcgcgttgc gcacgcaacg tcgccgtgtg ctcgtcgccg tcgaacagct catgatgcgc
     3301 ggcgagcgcg atctcgattt cgtcgtcgcc gggaagcgtc gtgcgctcgg ggagcatgag
     3361 ctggcgcgca gccttccgct tggcgagcga ccagtcggcg agtccgtgct cggcgatgag
     3421 ccgtgcggca agatcggcga tgcgtgcgcg attctgcgca tcgcggccct gcggctcacg
     3481 gtcgcggcgg ggtcggtgca cttcagaaaa gctggttgcg cacgtcgcgc gcgggggcgg
     3541 atcccggcat caatgccggc gcgagcgcgt cctgcgccat ccgcggcgtg aactcggcca
     3601 tgaaccagtc ggacaggttg cttgtatcgt cgcgcaatcc ggtctcggga ttgatgcgca
     3661 gcgaaacgac gccggacggc ggatcgcgca gcgtctcggg aacgcccttc agcgccttct
     3721 ccatgtacgc gatccacatc ggcagcgcgg ccacgcttcc cgtttcgttg gtgccgagcg
     3781 tcttcggctg gtcgtagccg atccacgcga tgcccacctg cgccacgttg aagccgcaga
     3841 accaggcgtc gacgtcgtcg ttggtcgttc cggtcttgcc ggcgatgtcc ttgcgcttca
     3901 acgacagcgc gcgcgtcgcg gtgcccgagg tgatcacctc ccgcaacagc gactgcatga
     3961 tgaacgcgtt gcgcgggtcg atggcccgct cggcatcctt gccggccatt gccggcttcg
     4021 cctcggacag cggatcgccg tggctgtcgg tgatccggct gatgaggtac ggagtgatgc
     4081 ggtagccgcc gttggcgaac accgaatacg cggtggccat ctgcatcggc gttgccgcac
     4141 ccgcgccgag gcccatcgtc aggtaggcag ggtgcagctt cgggtcgaag ccgaagcgcg
     4201 cgatgtagtc ctgcgcgtat tgcggaccga tcgcctgcag cacgcggatc gtgacgaggt
     4261 tcttcgagtg cgcgagcgcg acccgcagcc gcatcggacc ctcgaacttg ccctcgtagt
     4321 tcttcggctc ccacgcctcg ccgcccgcct tttcggccgg gacgaagaac ggcgcgtcgt
     4381 tgatgatcgt tgccggcgtg aagcccttct cgagcgccgc ggaatagatg aacggcttga
     4441 atgccgagcc cggctggcgc agcgcctgcg tcgcgtggtt gaacttgttg cgctcgtagt
     4501 cgaagccgcc gatcagcgcg agaatggcgc cgtcgtcggg gcgcacggcg acaaacgccg
     4561 cctccgcgag cggcatctgg gtgatcgcat agtgaccctt gtcgtcgcgg gtgaggcgga
     4621 tgatggcgcc ggtgcggatc ttctggttcg ccggcgcctt gtcgccgagc gcgcgctggg
     4681 cgaacttgag tccggcgccc gtcacttctg cctgatcgcc gttcgcaagc acggcgcgta
     4741 cctgcgagga cgtggcctgc agcacgacgc cggtgacgat attgtcgctg tcggggttct
     4801 cctggaacac gcggtcgagc gcggcatcct gctcggccgg gtccttcggc aggctcacgt
     4861 agccttccgg cccgcgatag ccgtggcgct ggtcgtggtc gatcacgcct ttgcgtaccg
     4921 ccgcgtacgc cgcttcctga tcggcgcggt ggatggtcgt ccacacggtg atgccgcgcg
     4981 tgtacgcgct gtcgccgtac gcgtcgaaga ccacctggcg ggccatctcg gccacgaatt
     5041 cggcgtgcac gccggacact tcgcgcgtcg ccgggcgcaa tttcaatggc gcattttgtg
     5101 cttcccgaaa ctggtcgtcg tttatatagt gaagctcatg catccgtcgc agcacgtaga
     5161 gctggcgcgt cttggccctg cgcggattgg tgatggggtt gtaggcggac ggcgccttgg
     5221 gcaggcccgc gagcgtggcg gcttcggcta cggtgatctc ggacagcggc ttgccgaagt
     5281 acgtctgcgc ggcggcggcg aagccgtagg cgcgctggcc gaggaagatc tggttgatgt
     5341 agagctcgag gatttcgtcc ttggacagcg aggcctcgat cttccacgcg agcagcgcct
     5401 cgcggagctt gcgcgacacc gtcttctcgc gggtgaggaa gaagttgcgg gcgacctgca
     5461 tcgtgatcgt gccggcgccc tgctgggtgc ccgacgtggc gttcgcgagc gcggcgcgaa
     5521 gcacgctcaa atagtcgacg ccgccgtgtt gatagaaacg ttcgtcttcc gcggccaaaa
     5581 tggcgtgttt catgacatcc gggacttcgg cgatacgcac gacggcgcgc cgctcctcgc
     5641 cgaattcgcc gagcaggtcg ccgtcggccg acaccacgcg caggggcacc ttcggctggt
     5701 agtcggtgag cgcgtcgaga ggggggagcg tcggccacag cagcgccagg acgaaggcgc
     5761 cgagcaggcc gccgacgacg gcgaggccca gcacgaccga ggcggtatag acgagccagc
     5821 gctttagcac gatggaataa aagccatgtt tggggcgggg tgccgcagtc gattataggc
     5881 gtggttgcaa cactcacccg ccggaaagga aacatttcgg cgaccggtac acttgtcgcc
     5941 ggcgtttaat gtagattatc agcaacgggg ccgacacgat gcttgccgat aacttctcct
     6001 ctgcactcga cttgttcaaa accaggtcgc cgacgcttat tggggtggat atcgcctcga
     6061 catcgctcaa gctcgtcgag ctttctgcgg ccgggaaggg gacttaccgc ctcgaccgct
     6121 acgtcatcga gccgctgccg aaggacgtcg tcgccgacgg caacgtgctc aacatcgagc
     6181 tttgcgccga cgtgctgaag cgcgcctgga agcgcctcgg cagccgcaat cgcaacgtcg
     6241 ccctcgcgct gcccgccgcc atggtcatca cgaagaaaat catcgtggcg gccggacagc
     6301 gggaggagga cctcgagctc accgtcgagg ctgaggccaa ccagtacatc ccgttcgcgc
     6361 tcgacgaggt caacctcgat ttccaggaga tcgggccggc cccgaacaac ccggacgaag
     6421 tcgaggtgct gatcgccgcg tcgcggaagg aaaaggtcga ggatcgcgtg gccgtggccg
     6481 aagcggccgg gctcaaggcg cgcgtaatgg acgtcgaatc gtattcgacg caggaggcgt
     6541 tccagctgat cgcgccgtcg ctgcccgcga acgggcgcga ccagaacatc gcgctcgtcg
     6601 acatcggggc gcacgtcatg cacttctacg tccttcgcaa cggccagatg ctattttcgc
     6661 gcgatcaggc gttcggcggc aatcagctta cgcaggatat ccagcgcgcc ttcaacctgt
     6721 cggccgacga ggccgagtcg gcgcgcagga accaggggtt gccggagaac tacgaccacg
     6781 acgtgctgca gccgttcatg gaatcgctcg cgctcgaggt gacgcgcgcg ctgcagttct
     6841 tcttcacgtc cacgtcgtac aaccaggtcg accagatcgt catcgccggc ggttgcgcgc
     6901 tgttgccggg gctcgacgag ctcgtcgcga agcgggccgg cgtcgccacc ctcgtcgcca
     6961 acccgttcgc cacgatgcag accagcgacc gggtccggcc gcggcagctc gcgcaggagg
     7021 cgccgatgct gatgatcgcc accggcctcg ccatgcgcgg tttcgaatag gacgaggggc
     7081 cttccatgcg catcaatctg ctgccccatc gcgagcagaa gcggcaagcc cgcgagcgcc
     7141 agttcgtatc gctcgcgatc acgctcgcga tcctcggcgt ggccatcgtg gcgctcgtgc
     7201 acgtgatcct cgccgcgcgc atcgacaacc agatctcgcg caaccagctt ctcaagacga
     7261 agatcgccga gctcgatgcg cagatcaagg agatcgaccg gctgcgcgac cagatccagc
     7321 aggtgctcgc gcgcaagcag gtcgtcgaaa cgctgcagtc gaaccgcaac gaggccgtgc
     7381 acctgctcga ccagctcgtg cgccaattgc ccgagggcgt ttacctgagc tcggtcaagc
     7441 agacggacaa gaaggtgacc atcgtcggct tcgcgcaatc gaatgcgcgc gtgtcgacgc
     7501 tgatgcgcaa catcgaaggc tcgccgtggc tcgagaagcc cgagctcgtc gagatcaggc
     7561 tcgtcagcgt tccggcacag ggccgcacgg gaccgcagaa cctgaaccag ttcacgctga
     7621 atttccagat ccggcgggcg acgcctgaag cggtcgcggc ccccggcgcg ccgggcaagg
     7681 cgcaggccaa gggaggcaag gcatgacgct cgacgacttc cgccgcctca atttccgcga
     7741 ggtcggcaag tggccactcg cgccgaagat catcgtcctc gcgctcgtcg tgctgttcat
     7801 cgtcgcgctc ggtgcctact tcgactggaa ggaccagtgg gattcgctgc aaaccgccca
     7861 gcaggaagag gggcggctgc gcgaccagtt caaggagaag aaggcgaagg cgatcaacta
     7921 cgacctctac gtgcagcagc tcgccgaggt cgagcagtcg ttcggtgcgc tcgtcaagca
     7981 gctgccgaac cgctcggaga tcgacgctct cctcaccgac atcaaccagg ccggcctcgg
     8041 ccgcggtctg cagttcgacc tgttccggcc ggcgccgcag gagcggatgg ccgacttcta
     8101 cgccgagctg ccgatcagca tcaagatcac cggcaactat cacgacatcg gcgcgttcgc
     8161 gagcgatgtt gccgcgctgc cgcgcatcgt gacgctgaac gacctcgcca tcgtcaacga
     8221 caagggaacg ctgacgatgg acgccgtggc gaagacgttc cgctatctcg acgaggaaga
     8281 aatggcgaag cagcgcgcgc tggcgaaggc ggcgaagggc aagaaatgaa gcgcggcgcc
     8341 ttcatcgcca ttgcatcggc cgcgctggtc gccggctgcg gtgcgtcgag ccacaaggat
     8401 ctcaccgact ggatggccga gcagggcaag ggcgtgcgcg gccacctcga ccccattccg
     8461 cagatcaagc cgtacgagcc gttcgtctac aacgatttcg acctgcccga tccgttcaag
     8521 ccgcgcaaga tcgagccgat gaagtccgag agcacgagca agctcgcgcc ggacctcaac
     8581 cgccgccgcg agccgctcga ggcctatccg atcgaatcgc tgtcgatggt cggcacgctc
     8641 gagaaaggca agacgcgcta cgcgctcgtc aagacacccg agcgcgacat ctaccaggtg
     8701 cgccaaggca tgcacatggg gcagaacttc ggcgtgatcg tcgacatcga cgacgccgac
     8761 atcaagctca aggaactcgt gcaggacggg tcgggggact ggtcggagcg gtccagcacg
     8821 ctcaacctgc agcaggctga ccagaaacag gagcagaaaa aatgaacgct tttggcgtcg
     8881 gtcgacggcc gggctcggga ggcctcgcca cagtgagagc atggctcgcg ggcatcgcgg
     8941 cgttcgtcgt tgccggtgcc gcactcgcgc aagctccgaa ccagatcgag caggtgtcgg
     9001 tcacccgtgc tgcatcgggc aacacgatcg tgcgcttcga gctcaagtcg ccgccggcca
     9061 atccgccggc aggcttcgcc accgcgtcgc cgccgcgcat cgcactcgac tttttcgaca
     9121 ccacgagcgc gctgccgagc aaccagcgca acgtcgacga tcccgcgctg cgcacgctcc
     9181 agttcgtcga agccggcaag cgcacgcgcg tggtgttcag cctgaacaag ccgcagtcgt
     9241 tcgacaccaa ggtcgaagga aacgccgtcg tcgtcaccct ctccgacagc acgtcggcgg
     9301 tggcgcaggc ggcgccggcg ccggtgcagc gcttcgccga aggcgccccg ggcgaccagc
     9361 agcacgcgct gcgcgacgtc gactttcgcc gcggcaggaa cggcgaggcg cgcatcatcg
     9421 tcgacctctc cgacaacaac acgggcatcg acatccgcca gcagggacgg caactgatcg
     9481 tcgacttcca gaagacgtcg gtgccgcgca acctcgagcg gcggctcgac gtcgccgatt
     9541 tcaacacgcc ggtcgtcacc gtcgacacgt tcgagcagaa cggcaacgcg cgcatggtga
     9601 tccagccgca gggcctgtgg gaacattcgg cgtaccaggc cgagaaccgc ttcatcatcg
     9661 aggtgaagcc ggtcgtcgac gatccgaaca agctcgtgca gggatcgcgc gccggctaca
     9721 agggcgagaa gctgtcgctc aatttccaga acatcgaggt gcgctcggtg ctgcaggtga
     9781 tcgccgactt caccggcctc aacatcgtca ccagcgacac ggtcacgggc agcctcacgc
     9841 tgcgcctcaa ggacgtgccg tgggaccagg cgctcgacat catcctgcag accaagggcc
     9901 tcgacatgcg caagaacggc aacatcgttc tgatcgcgcc gcgcgaggaa ctggcgctga
     9961 aggaaaagca gcagctcgag tcgcaggcgc agatcaacga gctcgagccg ttgcagaccg
    10021 aaacgttcca gctcaattac gcgaaggcca acgacatcct ggccatcatc aacggccgct
    10081 ccagcgccgg cggcggcgcg ccgggtgccg cggcagtcgc gcaattcctg tccaagcgcg
    10141 gcaccgcgtt cgtcgatccg cgcaccaaca tcctgttcgt caccgacatc gcgagccggc
    10201 tcgaggaagt gcgacgcgtg atccgccaga tcgacacgtc ggtgcgccag gtgctgatcg
    10261 aggcgcgcat cgtcatcgcc gacgacacgt tcagccggca gctcggcatc cgcttcggcc
    10321 agcagactgg cgcgacgctc ttcggccggc gctattcgct gggcagcggc ggctcgcttg
    10381 caacgcagcc ggtggtctcc ctgtcgggcg gcgcgggcgg cacggtgacg cgcaccaccg
    10441 aaacgcagac gccgttcgag ctcgcgtcgg gtatcgccac gccgggctac tcgaacgatc
    10501 cgcagctcaa catcaacctg cccgtcgcca atcccgccgg gcagctggcg ctcacgctga
    10561 tcaacctcgg tagcggcaac ctgatcaacc tcgagctgtc ggcactcgaa gccgacaacc
    10621 gtggcaaggt cgtgtcgagc ccgcgcgtga tcaccggcga caaccagaag gcgcatatcg
    10681 agcaggggac cgaaattccg tacgtgacgc cgggcagcgc caattcgccg gcgacggtgt
    10741 cgttcaagaa agcggtgctc gcgctcgatg tcacgccgca gatcaccccc gacggacgcg
    10801 tgatcatgca ggtcgagatt cgcaaggact cggtcggcca gctcgtcaac gtgcagggtg
    10861 gcggacaggt gccggcgatc gacacgaaga acgtgctgac gcagatcgcc gttaacaacg
    10921 gcgacaccgc cgtcatcggc ggcatctacg aggaagtgat ccgcaacgac aagacgcagg
    10981 tgcccttcct cggtgatgtg ccggtgctcg gcaatctgtt caagacgacc acgcgctcga
    11041 gccagaagac cgagctgctg atcttcctga cgccgcgcat cgtgaaggac accgtgacgt
    11101 cggtcaggta aaccgatgta gggtcagaga cgactttcct cgcggaaact cgtctccgac
    11161 cctttacaat tccccggtgt cactgcaccg gggcaatctt tttctcgtgg gcctgatggg
    11221 cgccggcaag acgacgctcg gacgccagct cgcgcgccgc ctcgacaagc cgttcgtcga
    11281 cgccgaccac gagctcgagg cacggctcgg ggtgcgcatc ccgacgatct tcgaggtgga
    11341 aggcgaagcc ggctttcgcg atcgcgagga agcggtgatc gacgagctct cgcgccgcag
    11401 cggcatcgtg ctcgcgaccg gaggcggggc cgtcatgcgc gagcaaagcc gcgtgcgact
    11461 gcgcgagaac ggcaccgtgc tgtacctgca cgccgagccc gagacgctct ggcaacgcct
    11521 gcgcaacagc cgccagcgtc cgatgctgca cgccgccgat ccacgcaacc ggctggtcga
    11581 gctgtaccag ctgcgcgatc cgctctaccg cgaggtggcc gaccacgtga tcgagtcgga
    11641 ccgcgaagcg gtcatccgct tcgtgcgctg gctcgacgcc gagccttgca acggatcgcc
    11701 gggatgacct tccctttccg gctggcatga agacattgac ggtcggcctc ggcgcgcgca
    11761 cgtacccgat tcacgtcggc aatgcgctgc tgtcgcgcgc cgcgtcgctg ctcgacgtgc
    11821 agcgcggaca gcgcggcgtg atcgtgacga acgcaatcgt cgcggcacac caccttgcgc
    11881 ccttgagaac gtcgctgggc ggcgccggca tgcacgtcga cgtcgtgctg ctgcccgacg
    11941 gcgaggcgca caagaaccag aaaacgctcg acgatcttct gacgcgcctc ctcgaattgc
    12001 gcatcgagcg ttcgacgacg ctgattgcgc ttggcggcgg cgtcgtcggc gacatcgcgg
    12061 gcttcgcggc ggcgatctac cagcgcggca ttgcgttcgt ccaggtgccg acgacgctgc
    12121 tcgcgcaggt cgactcctcg gtgggcggca agaccgggat caaccatccg ctcggcaaga
    12181 acatgatcgg cgcgttctgg cagccgcgcg ccgtgctgat cgatacggcc gtgctgtcga
    12241 cgttgcccga tcgcgagttg tcggcgggac tggccgaggt gatcaagtac ggcgcgatcc
    12301 gcgatgcaga attcttcgcg tggctcgaaa cgaacatggc ggcgctgctt gcgcgcaacg
    12361 acgcagcgct cgaacgcgcc atcgtgcgca gttgcgagat caaggcgcag atcgtcgccg
    12421 ccgacgagcg cgaagcgggc gagcgcgcac tcctcaactt cggtcacacg ttcggtcatg
    12481 cgatcgaggc cgcacagggt tacggcgaat ggctgcacgg cgaggccgtc gcggccggca
    12541 tggtgtgcgc ggcgcgattg tcggggcgcg tgtgtggatt gagcgcggac gaagccacgc
    12601 gcctcgccaa cctggtcgtt gctgcgaaat tgccggcggc gccaccgagg atcgcgcgcg
    12661 agcgctggct cgagttgatg cagcgcgaca agaaggttca atcaggagcg atgcgcttcg
    12721 tgctgctcga acgactcggt cgtgcggtcg tgcgcggcga cgttgccgag aacgacgtgg
    12781 tgcagagcgt gaaacccgac gcgatgcgct gaaccgggtc acctcggcgc acctgctatt
    12841 tcttccgacg gcgtggcaac aaaacggcg
//
LOCUS       DASSVX010000305         9601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_796924, whole genome shotgun sequence.
ACCESSION   DASSVX010000305 DASSVX010000000
VERSION     DASSVX010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9601
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796924"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFO53_13105"
     CDS             <1..364
                     /locus_tag="VFO53_13105"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HET8877263.1"
                     /translation="ARARTFAFMQDVEAMRAAGLALGGSLHNAIVLDETRVLNSEGLR
                     YDNEFVKHKVLDAIGDLYLLGRPLIGQYTAFKPGHALNNALTRALLARSDAWEIVSFD
                     ADDELPSAFQDWAAIGAA"
     gene            361..561
                     /locus_tag="VFO53_13110"
     CDS             361..561
                     /locus_tag="VFO53_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877264.1"
                     /translation="MILVRLLLFAALATVGAAVLLWLVKRDPRYLRFIGLTLKFTVLL
                     LLAILLWFAAERLLAPMLGPLL"
     gene            575..2092
                     /gene="cysC"
                     /locus_tag="VFO53_13115"
     CDS             575..2092
                     /gene="cysC"
                     /locus_tag="VFO53_13115"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HET8877265.1"
                     /translation="MTMPEAPHAESPPLRVLVCGGAQQGKRTLLARLAGEHGPAGAIR
                     SPQASAALAARFVLVNTPEDERDVRRMVGEDAAADVGVFVVDAGSALLEPAHRFGFVA
                     ALLCLPRVVLAVNKMDLAGNAQSAFTAIETVYQALVHELGFARVAAVPISALHGDNVA
                     EPGAAMDWYAGPSLVAHLDGAAIDEAREPASGVATASRPEADQFEASIAWFGEAPLLR
                     GRRYAMTIAATTVNATIAPLKYRIGLDSLEHVAADKLERDEVGVGDLTLAQPIAFTPA
                     GGGRAGERFVLIDPMNGDIVGAGRLRFALRRSQNIHWQPVDIDRVARASLKSQRPCVL
                     WFTGLSGAGKSTIANLLEGRLHALGRHTYLLDGDNVRKGLNKDLGFTAEDRVENIRRI
                     AEVAALMADAGLIVITAFISPFRNERRMARALLPDGDFIEIYVDTPLAVAERRDPKGL
                     YRKARRGELANFTGIDSPYEAPLHAELTIDGAAADPASAVERIIAQLRRCGIIAA"
     gene            complement(2079..2360)
                     /locus_tag="VFO53_13120"
     CDS             complement(2079..2360)
                     /locus_tag="VFO53_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877266.1"
                     /translation="MALYPDHYNVPLHLIASEGEIDPRWLAHAPVHLHYHWMFDERHH
                     ENAMELLARLGVPADRRAWLLARTPFRAAAATRAGAPAEHGTPRALTPR"
     gene            complement(2318..5251)
                     /locus_tag="VFO53_13125"
     CDS             complement(2318..5251)
                     /locus_tag="VFO53_13125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8877267.1"
                     /translation="MNRNAPCACGSGKRFKHCCGQDPVRAESAAPARDRALAAHRAGE
                     LGKAESLYRLALDDNPADVDALHMLGIVQFERMRYREALQLLWDAAERTGWTIAAIRH
                     NLGLVLGKLLTREANVRQADLLAASIARARSEPHADASPRVSVVLPAFNHAAYVADAI
                     TSVAAQTYANLELVVIDDGSDDPTPAVIAECLASFPRPSRFVRRENRGAPATLNEAAA
                     LAGGGYLAFLNSDDAYAPGRLAALVDAVARRGHAWGFSLVTNAFAGDSARADAAADVL
                     QKQRNFLGSQPASFTLVEFNVAVSSGNLFVEREFFRSLGGFRDYRYNHDWDFCLRAAA
                     LAEPVVVEQPLYHYRRHASNTIAESRLRATQDADSVFADFVAAALSDGSGARNDLGPW
                     WPANRTLLLRQLLRNGQGALVPVAVLRALADAWRSAPVPADRAASTRAVAGTERKSAL
                     VVLGMHRSGTSAIARVLNLCGAFLPAKVKPPKIGVNPKGFWEPEAVLDLDVRLMRQLG
                     GDWCHVDFALPGSGALVDEFLADAGALLASEYEDHALIAIKDPRICVLAPLWHRALEA
                     AGYRPLYVVPIRNPLEVAQSLHARGDMSIDAGLALWRAYMERVVAFTDDRADVVFIRY
                     AELLDDWQAVVERIARRLDVPLDVERDAGEVQRFLDKDLRNQAADEHALDMLLPGTEG
                     DAIRALHRTCLAHCDRDASPRTQARSSRDGAGDTVRLGGPHASACFVLCIENNGIREQ
                     ALLLCRSVRQFAGRYRDAPILAYAPRPGLGVGASVRRALTDLGVEYIDAPLNTTCPEY
                     PPANRVFAGAHAERHADADFLIVCDSDTVWLGEPELPDDADAGVRAVDSKGSATGGPG
                     DRFDDYWAQLAQLAGVPLDRLPYLRSTIGNERIRASYNAGFTIARRRLGMLTRCAELF
                     AASRDAGLRPYRDSGLEIVASTGRVGRVGSEYWGSSQAALPLPSGPRPIAWLCTPITT
                     TFRCT"
     gene            5417..6409
                     /locus_tag="VFO53_13130"
     CDS             5417..6409
                     /locus_tag="VFO53_13130"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET8877268.1"
                     /translation="MKPSPDPYTRRVIIAVALVALAFIVWQLRGVLPLVFGGVILATA
                     LRALADAVSHATALPARGALAAVVVALLCAIGLGVWLVGAQIAGQLAGLWQALPQALG
                     AASDWLARTPFSSVLSGTWQSIKDGGVPWLRVAGAAGVATDALLDAVLILAIGLYVAA
                     DPRSYFAGALRLAPPAYRGRIGAALSEAGHALRRWLLGQLVSMITIGAMTTIGLWLLG
                     VPLALSLGLIAGVSEFVPFFGPIAFGIFAVLFAFTQGPSQALYVGLLCFGIQHFEGYV
                     LQPLIQRWAVALPPALAVLSVVIFALLFGVLGAILATPLMTVVVILVERFYEGE"
     gene            complement(6433..6897)
                     /locus_tag="VFO53_13135"
     CDS             complement(6433..6897)
                     /locus_tag="VFO53_13135"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HET8877269.1"
                     /translation="MSDRPRVTRKTPHQTLARVLDADATIGAWTARQREEAALTGLVR
                     RMLPRPIAERVRVTDARNGVLELAATAGAIAAAVRQRAPDLRAALGREGRAFAEVRVR
                     VQVTGSVIARDKAAVRAWDSSAAAPIFDLADRLPEGPLQQALRRWSRRARGR"
     gene            7194..>9601
                     /gene="secA"
                     /locus_tag="VFO53_13140"
     CDS             7194..>9601
                     /gene="secA"
                     /locus_tag="VFO53_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET8877270.1"
                     /translation="MINILTRIFGSRNERLLKQYAQQVRDINALEPAISALADDALRD
                     KTNELKTRVAEGATLDDVLPEAFAVVREAGKRTLNMRHFDVQLIGGIALHNGKIAEMR
                     TGEGKTLVATLPAYLNALSGKGVHVVTVNDYLAQRDADWMGRIYRYLGLTVGVNLSQM
                     SHADKQVAYAADITYGTNNEFGFDYLRDNMVFATSERVQRGLNYAIVDEVDSILIDEA
                     RTPLIISGQSDDNVDIYYRLNELPPKLTRQPEEKGPGDYWVDEKGHQVLLSEEGHEKA
                     EQLLASAGLTAPGTSLYDAQNISLVHHLYAALRAHALFHRDQHYVVQNGEVIIVDEFT
                     GRLMSGRRWSDGLHQAVEAKEGVPIQRENQTLASITFQNYFRMYAKLAGMTGTADTEA
                     FEFQQIYHLETMVIPTHQPMVRKDENDLVFRTLAEKVTAIVADVKDCHERGQPVLVGT
                     TSIENSEMLSMHLDKERLPHQVLNAKQHEREAEIVAQAGRPGVITIATNMAGRGTDIV
                     LGGTIEPHLLKLREDATLAPADKEQQIGTLRGEWQGRHDAVIKAGGLHIVGTERHESR
                     RIDNQLRGRSGRQGDPGSSRFYLSLEDPLLRIFGGERLSGIMQRLKMPEGEPIEHSIV
                     NRSIAKAQNRVESRNFDIRKQLLEYDDVANDQRRVIYQQRNELLESDEVADTIASMIR
                     GEIDATFKRHIPPESVEEQWDIPALESTLGSEYQLAAPVREWLKAEPELDDAALRERI
                     EKLASEAWHGKEAAVGTELMRQFERSLMLQTLDHHWREHLASLDHLRQGIHLRGYAQK
                     NPKQ"
BASE COUNT         1522 a         3261 c         3340 g         1478 t
ORIGIN      
        1 tgcgcgcgca cgcacgttcg cgttcatgca ggatgtcgag gccatgcgcg ccgccggcct
       61 cgcacttggc ggcagcctgc acaatgcgat cgtcctcgac gagacgcgcg tgctgaacag
      121 cgaggggctg cgctacgaca acgagttcgt caagcacaag gtgctcgacg cgatcggcga
      181 tctctatctt ctcgggcgtc cgctgatcgg acagtacacg gcgttcaagc cgggccatgc
      241 gctcaacaac gcgctcaccc gcgcgctcct cgcgcgaagc gacgcctggg agatcgtctc
      301 gttcgatgcc gacgacgaac tgccgtccgc gttccaggac tgggcagcca tcggcgcagc
      361 ttgatactgg ttagattgct gctgttcgcg gcactcgcga cagtgggagc ggcagtgctg
      421 ctctggctcg tcaagcgcga cccgcgctac ctgcgcttca tcgggctcac gctcaagttc
      481 accgtgttgc tgctgctcgc gatcctgctg tggttcgcgg cagaacggct gctcgcaccg
      541 atgctcgggc cgttgctgta acgacgcgag cgtcatgacg atgccggaag caccgcacgc
      601 cgagtcacca ccgttgcggg tgctcgtgtg cggcggcgcg cagcaaggga agcggacgct
      661 gctcgcgcgg ctcgccggcg agcacgggcc ggccggtgcg atccgatccc cgcaggcaag
      721 cgcggcattg gcagcgcggt tcgtcctcgt aaacacgccg gaggacgagc gcgacgtgcg
      781 gcgcatggtg ggcgaggacg ccgcggcgga cgtcggagtg ttcgtcgtcg acgcaggcag
      841 cgccttgctc gagccggcgc accgctttgg cttcgtggcc gcgctgcttt gcctgccgcg
      901 cgtggtgctt gcggtcaaca agatggatct cgcgggcaac gcgcaatcgg cgttcactgc
      961 gatcgagacc gtctatcagg cgctcgtgca cgagctcggg tttgcgcgcg ttgccgccgt
     1021 tccgatatcg gcgctgcacg gtgacaacgt tgcggaaccc ggtgcggcga tggactggta
     1081 tgcgggccca tccctggtcg cgcacctcga cggcgctgcc atcgacgaag cgcgcgagcc
     1141 cgcgtccggc gtggcaacgg cgtcgcgtcc cgaggccgat cagttcgagg cgtcgattgc
     1201 ctggttcggc gaggcgccgc tgttgcgcgg gcggcgctac gcgatgacga ttgcggcgac
     1261 gacggtcaat gcgacgatcg cgccgctcaa atatcgcatc ggcctcgact cgctcgagca
     1321 cgtcgccgcc gacaagctcg aacgcgacga agtcggcgtc ggcgacctca cgctcgcgca
     1381 gccgatcgcg ttcacccccg ccggcggcgg ccgggctggc gagcgcttcg tgctgatcga
     1441 cccgatgaac ggcgacatcg tcggcgcggg ccggctgcgc tttgcgctgc gccgctcgca
     1501 gaacattcat tggcagccgg tcgacatcga tcgcgtcgcg cgggcgtcac tcaagtcgca
     1561 gcggccctgc gtgctgtggt tcaccggcct gtcgggggcg ggaaagtcga cgattgccaa
     1621 tctgctcgag ggccggctgc acgcgctcgg acggcacacg tatctgctgg acggcgacaa
     1681 cgtgcgcaag ggcctcaaca aggatctcgg tttcaccgcc gaggatcgcg tcgagaacat
     1741 tcgccgcatc gccgaggtcg cggcgctgat ggccgacgcg ggcctgatcg tcatcaccgc
     1801 gttcatctcg ccgtttcgta acgagcgacg gatggcgcgc gcgctgctgc ccgatggcga
     1861 cttcatcgag atctacgtcg acacgccgct ggcggtggcc gaacgacgcg accccaaggg
     1921 cctctaccgg aaggcccgcc ggggcgagct cgcgaacttc accggaatcg actcgcccta
     1981 cgaggcaccg ctccacgccg agttgaccat cgacggtgcg gctgccgatc cggcatcggc
     2041 ggtcgagcga atcattgcgc aactgcgacg ctgcggcatc atcgcggcgt gagcgcccgc
     2101 ggcgtgccgt gttccgcagg cgcgccggcc cgggtcgcgg ccgcagcgcg aaacggcgtg
     2161 cgcgcgagca gccatgcgcg ccgatcggcg ggaacgccga gccgcgccag cagttccatg
     2221 gcgttctcgt ggtggcgttc gtcgaacatc cagtggtagt gcagatgcac cggtgcgtgc
     2281 gcgagccagc gcgggtcgat ctcgccttcg gacgcgatca ggtgcagcgg aacgttgtag
     2341 tgatcggggt acagagccac gcgatcggtc gtggcccaga tggcaagggt agcgccgcct
     2401 ggctcgagcc ccagtactcg cttccgacgc gcccgacgcg ccccgtcgat gcgacgattt
     2461 cgaggccgct gtcgcgatag ggacgaagcc ccgcatcgcg cgacgcggcg aagagctcgg
     2521 cgcagcgcgt gagcatgccg aggcgccggc gcgcgatcgt gaagccggcg ttgtacgagg
     2581 cgcggatgcg ctcgttgccg atcgtcgagc gcagatacgg gaggcggtcg agcggcacgc
     2641 cggccagttg cgcgagttgc gcccagtagt cgtcgaaacg atcgccgggc ccgccggtcg
     2701 cgctgccctt cgaatcgacg gcgcgcacgc cggcgtcggc atcgtcggga agctcgggct
     2761 cgccgagcca gaccgtgtcg ctgtcgcaca cgatcaggaa atcggcatcg gcgtggcgct
     2821 cggcgtgcgc gccggcgaac acgcggtttg ccggcgggta ctcggggcat gtcgtgttga
     2881 gcggcgcgtc gatgtactcg acgccgaggt cggtgagcgc ccggcgcacc gacgcgccga
     2941 cgccgagccc cgggcgcggc gcgtacgcga ggatcggcgc atcgcgatag cgcccggcga
     3001 actgccggac gctccggcac agcagcagcg cctgctcgcg gatgccgttg ttctcgatgc
     3061 acagcacgaa gcacgcgctg gcgtgcgggc cgccgaggcg aaccgtgtcg cccgcaccgt
     3121 cgcggcttga ccgcgcctgc gtacgcggcg acgcatcgcg gtcgcagtgc gcaagacacg
     3181 tgcgatggag cgcgcggatc gcgtcgcctt ccgttcccgg caggagcatg tcgagcgcgt
     3241 gctcgtcggc cgcctggttg cgcaggtcct tgtccagaaa gcgctgcacc tcgccggcgt
     3301 cgcgttccac gtcgagcggc acgtcgagcc gccgcgcgat ccgttcgacg accgcttgcc
     3361 agtcgtcgag caattcggcg tagcgaatga acacgacgtc ggcgcgatca tcggtgaacg
     3421 cgacgacccg ctccatgtac gcgcgccaca gcgcgagacc ggcgtcgatc gacatgtcgc
     3481 cgcgcgcgtg cagcgattgc gcgacctcga gcgggttgcg gatcggaacg acatacagcg
     3541 gtcgatagcc ggcggcttcg agtgcgcgat gccacaacgg cgcgagcacg cagatgcgcg
     3601 gatccttgat cgcgatcagc gcgtggtcct cgtactccga tgcgagcagg gcgccggcat
     3661 ccgccaggaa ctcgtcgacg agtgcaccgc ttccgggcag cgcgaagtcg acgtgacacc
     3721 agtcgccgcc gagttggcgc atcaggcgca cgtcgagatc gagcaccgct tcgggctccc
     3781 agaagccctt gggattgacg ccgatcttcg gtggcttgac cttggcgggc aggaatgcgc
     3841 cgcacaggtt cagcacgcgg gcgatcgccg acgtgccgct gcgatgcata cccagcacga
     3901 cgagtgcgct cttgcgttct gtgccagcaa ccgcccgcgt gctcgccgcg cggtcggcgg
     3961 gcaccggcgc cgagcgccat gcatcggcga gcgcgcgcag gacggcaacg ggaacgagcg
     4021 cgccctggcc gttgcgcagg agctggcgca acagcagcgt ccggttcgcg ggccaccagg
     4081 gaccaagatc gttgcgcgcg cccgacccgt cgctcaatgc ggcggcgacg aaatcggcga
     4141 agaccgagtc ggcatcctgc gttgcgcgta gcctcgattc tgcgatcgtg ttgctcgcgt
     4201 gccggcgata gtgatagagc ggctgctcga cgacgacggg ctcggcgagc gctgccgcgc
     4261 gcaggcaaaa atcccagtcg tggttgtacc gatagtcgcg gaatccgccg agcgagcgga
     4321 agaattcgcg ctcgacgaac aggttgccgc tcgacaccgc gacgttgaat tcgacgagcg
     4381 tgaagctcgc cggctgcgag cccaggaaat tgcgctgctt ctgcaggaca tcggccgctg
     4441 cgtcggcccg cgccgagtcg ccggcgaacg cgttggtgac gagcgagaag ccccacgcgt
     4501 ggccgcggcg cgcgaccgcg tcgacaagcg ctgcgaggcg gccgggcgca tacgcgtcgt
     4561 cggagttcag gaacgcgagg tagcctccgc ccgcaagcgc cgcggcttcg ttgagtgttg
     4621 cgggcgcgcc gcgattttca cgccggacga agcgcgacgg ccgcggaaac gacgcgagac
     4681 attcggcgat caccgccggc gtgggatcgt ccgagccgtc gtcgatgacc acgagctcga
     4741 gattcgcgta cgtctgcgcc gccaccgagg tgatcgcgtc ggcaacgtac gccgcgtggt
     4801 tgaaggccgg cagaacgacg ctcacccgcg gcgacgcatc ggcgtgcggc tcgctccgcg
     4861 cacgggcgat cgacgccgcg aggaggtcgg cctgtcgcac gttcgcctcg cgcgtcaaca
     4921 gcttgccgag gacgagcccc aggttgtgcc ggattgccgc aatcgtccag cccgttcgct
     4981 cggccgcatc ccacaggagc tgcagcgcct cgcggtagcg catccgctcg aactgaacga
     5041 tgccgagcat gtgcagcgca tcgacgtcgg cgggattgtc gtcgagcgca agccggtaga
     5101 gcgattccgc ttttcccagt tcaccggcgc gatgggccgc aagcgcgcgg tcgcgcgccg
     5161 gcgcggcgga ctcggcgcgc accgggtcct gtccgcagca gtgcttgaat ctcttgccgc
     5221 tgccgcaggc gcagggcgca ttgcgattca tcgggtgaac tccatggaca cgccgacgcg
     5281 cgggccggcc ggcgtcgagg cggcattatc gccgcaacgc gacgcgccgc cgacgggcaa
     5341 tgtgagcggg agcccgacag ccgaatggtc gtcgttccga tgacgtggcc ggacactgcg
     5401 gcttaacgtc ggacgcttga agccgagccc cgacccgtac acgcggcgcg tgatcatcgc
     5461 ggtcgcgctg gtcgcgctcg cgttcatcgt ctggcagttg cgtggcgtgc tgccgctcgt
     5521 gttcggcggc gtgattctcg ccacggcgtt gcgcgcactc gccgacgccg tctcgcacgc
     5581 cacggcgcta ccggcgcgcg gggcgcttgc cgcggtggtc gtcgcacttc tttgcgcgat
     5641 cggactcggc gtatggctcg tcggcgcgca gattgccggg cagttggcgg ggctgtggca
     5701 ggcgctgccg caggcgctcg gtgcggcgag cgactggctc gcccggacac cgttctcgtc
     5761 ggtgctttcg ggcacctggc agtcgatcaa ggacggcggc gttccctggc tgcgggttgc
     5821 cggcgcggcc ggcgtcgcga ccgatgcgct gctcgatgcg gtgctgatcc tggctatcgg
     5881 tctctacgtc gcggccgatc cgagatcgta tttcgccggt gcgctacgac ttgcgccgcc
     5941 cgcctatcga ggccggatcg gcgctgcgct gtccgaggca gggcatgcgc tccgccgctg
     6001 gcttctcggt cagctcgtct cgatgatcac gatcggcgcg atgacgacga tcggcttgtg
     6061 gctgctcggc gtaccgctgg cgctgtcgct cggcctgatc gccggcgtca gcgagttcgt
     6121 gccgttcttc gggccgatcg cgttcggcat cttcgcggtg ctgttcgcgt tcacccaggg
     6181 cccgagccag gcgctgtacg tcggcctgct gtgcttcggg atccagcact tcgaaggcta
     6241 cgtgctgcag ccgctgatcc agcgctgggc ggtggcgctg ccgcccgcac tcgcggtgct
     6301 gtcggtcgtc atcttcgcat tgctgttcgg cgttctcggc gcgattctcg caacaccgct
     6361 gatgacggtc gtcgtgattc tcgtcgagcg cttctacgag ggcgagtgac gcgggaccgg
     6421 tccgtcacgc gctcaacgtc cccgcgcacg tcgcgaccag cggcgcagcg cctgctgcaa
     6481 cgggccctcg ggcaggcgat cggcgagatc gaagatcggc gccgccgcgc tgctgtccca
     6541 cgcgcgcacg gcggccttgt cgcgggcgat caccgacccg gttacctgca cgcgcacgcg
     6601 cacttcggcg aacgcgcggc cctcgcgccc gagcgccgcc cgcagatcgg gcgcccgctg
     6661 tcgcacagcg gcggcaatgg cgcccgccgt cgcggcgagt tccagcacgc cgttgcgggc
     6721 atcggtcacg cgaacgcgtt cggcgatcgg ccgcggaagc atccggcgca cgaggccggt
     6781 cagcgccgcc tcttcccgct ggcgcgcggt ccatgcgccg atcgtggcgt cggcgtcgag
     6841 cacgcgggca agcgtctggt gcggcgtctt gcgggtcacg cgagggcgat cggacatgga
     6901 aaggcgaccg cctatgataa aatgcgcgcc gatcgaccat ggcaataccc gctcgccggt
     6961 gtcgcgattg aacatgcagg tggccagatg ggtcgtacgc gcaagccccg tcgcggtcgt
     7021 ggcgcgttca gtcccggccg gcgtgcggtc gaacgacgtg ccgtgttgtc cggcgcgatt
     7081 cctgcaagcc acgggctgac gcttcccgat ccggaaccgg cggccacagg aggggtgagg
     7141 cggtcctcac cccttgcttt ttgcccgcgc cgcgccaagc ttccctctcc gcgatgatca
     7201 atatcctcac ccgcattttc ggcagccgca atgagcggct cctgaagcag tacgcgcagc
     7261 aggtgcgtga catcaacgcc ctcgagccgg cgatttccgc cctggcggac gatgcgctgc
     7321 gcgacaagac gaacgagctc aagacgcggg tggcggaggg cgccacgctc gacgacgtgc
     7381 tgcccgaggc gttcgccgtc gtccgcgaag cgggcaagcg cacgctcaac atgcgccatt
     7441 tcgacgtgca gctcatcggc ggcattgcgc tgcacaacgg caagatcgcg gaaatgcgca
     7501 cgggtgaagg caagacgctc gtggccacgt tgcccgcgta cctgaatgcg ttgtcgggca
     7561 agggcgtgca tgttgtcacc gtcaacgact acctcgcgca gcgcgatgcc gactggatgg
     7621 ggcgcatcta tcgctacctc gggctcaccg tcggcgtcaa tctgtcgcag atgtcgcacg
     7681 ccgacaagca ggtcgcgtac gccgccgaca tcacctacgg caccaacaac gaattcggct
     7741 tcgactacct gcgcgacaac atggtattcg cgacgagcga gcgcgtgcag cgcgggctca
     7801 actacgcgat cgtcgacgag gtcgactcga tcctgatcga cgaggcgcgc acgccgctca
     7861 tcatctcggg ccaatccgac gacaacgtcg acatctatta ccggctgaac gagctgccgc
     7921 cgaagctcac gcgccagccc gaggaaaagg gaccgggcga ctactgggtc gacgagaagg
     7981 ggcatcaggt cctgctgtcg gaagaagggc acgagaaggc ggagcagctc ctcgcgtcgg
     8041 ccggcctcac ggcgcccggg acgagcctct acgacgcgca gaacatctcg ctcgtgcatc
     8101 acctgtacgc tgcgctgcgc gcgcacgcgc tgttccatcg cgaccagcac tacgtcgtgc
     8161 agaacggcga ggtgatcatc gtcgacgagt tcaccggccg cctgatgtcc ggcaggcgct
     8221 ggtccgacgg cctgcaccag gcggtcgagg cgaaggaggg cgtgccgatc cagcgcgaga
     8281 accagacgct cgcctcgatc acgttccaga actacttccg catgtacgcg aagctcgcgg
     8341 gcatgaccgg caccgccgac accgaggcgt tcgagtttca gcagatctat cacctcgaga
     8401 cgatggtgat cccgacgcac cagccgatgg tgcgcaagga cgagaacgac ctcgtttttc
     8461 gcacgctggc cgaaaaggtc acggcgatcg tcgccgacgt caaggattgc cacgagcgcg
     8521 ggcagccggt gctcgtgggc acgacgtcga tcgagaactc cgagatgctg tcgatgcacc
     8581 tcgacaagga gcggctcccg caccaggtgc tgaacgcgaa gcagcacgag cgcgaggccg
     8641 agatcgtcgc gcaggcgggc cgtcccggcg tgataacgat cgcgacgaac atggccggcc
     8701 gcggcaccga catcgtgctc ggcggcacga tcgagccgca tctgctgaag ctgcgcgagg
     8761 atgcgacgct cgcgcctgcc gacaaggagc agcagatcgg cacgctgcgc ggcgaatggc
     8821 aggggcgtca cgatgcggtg atcaaggccg gcggcctgca tatcgtgggc accgagcggc
     8881 atgagtcgcg ccgcatcgac aaccagcttc gcggccgttc cgggcgccag ggcgaccccg
     8941 gctcgtcgcg gttctacctg tcgctcgagg atccgctgct gcgcatcttc ggcggcgagc
     9001 gtctgtccgg catcatgcag cggctcaaga tgccggaagg cgagccgatc gagcactcga
     9061 tcgtcaaccg ctcgatcgcc aaggcgcaga accgcgtcga atcgcgcaac ttcgacatcc
     9121 gcaagcagct tctcgaatac gacgacgtcg cgaacgatca gcggcgcgtg atctaccagc
     9181 agcgcaacga gctgctcgaa agcgacgaag tcgccgacac gatcgccagc atgattcgcg
     9241 gcgagatcga cgcgacgttc aagcgccaca tcccgccgga gtcggtcgag gagcaatggg
     9301 acataccggc actcgaatcg acgctcggct ccgaatacca gctcgccgca ccggtgcgcg
     9361 agtggctgaa ggccgagccc gagctcgacg acgccgcgct gcgcgagcgc atcgaaaagc
     9421 tggcgagcga agcctggcac ggcaaggaag cggccgtcgg caccgagctc atgcgccagt
     9481 tcgagcgcag cctgatgctg cagacgctcg accatcactg gcgcgagcac ctcgcatcgc
     9541 tcgaccatct gcggcagggc atccacctgc gcggctacgc gcagaagaac ccgaagcagg
     9601 a
//
LOCUS       DASSVX010000306         6041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1113661, whole genome shotgun sequence.
ACCESSION   DASSVX010000306 DASSVX010000000
VERSION     DASSVX010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113661"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2140
                     /locus_tag="VFO53_13145"
     CDS             <1..2140
                     /locus_tag="VFO53_13145"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8877271.1"
                     /translation="ELEFLVGLSMAAAVAMENARLFAQAEQRATELDTVNTVSQQVAG
                     KLDISALIELVGEQVRRVFKPDMAYVALLDRATEMIHFPYRHGDVFGSRRRGEGLTSQ
                     IIDTGAPLLLNSDESLNVAAGTKRLGKHALSYLGVPIVVDGRAEGVISVQSTQREGVF
                     DANDQRLLETIAASVGVALRNAQLFAEAREARAGAEAANEAKSSFLATMSHEIRTPMN
                     AVIGMSGLLLDTPLSDEQREYAATIRDSGDALLTIINDILDFSKIEAGRMDIEAQPFD
                     LRECVESALDLVGPRAAQKQLDLAYLFEGDVPAVVDGDVTRLRQILLNLLSNAVKFTE
                     RGEVVLTVTPDTLDGTPMLTFAVRDTGIGLSPEAIGRLFQKFTQADASTTRKYGGTGL
                     GLAISKRLAELMGGTMSAASDGPGRGATFTFTINAPPTVLPAAGRREFIGAQRELRGR
                     RVLVVDDNATNRRVLKLQMAKWGMVPADADAPDEALHRVLDGHMFDLAILDMHMPQMS
                     GVELARRLRAARPALPLVLFTSLGRKEAGDVDGLFNAYLAKPLRQSQLFDTLVTLLAH
                     DASPKVAPAARPRLDAGMADRHPLRILVAEDNVVNQKLALRLLQQMGYRADVASNGIE
                     AIESIERQPYDIVLMDVQMPEMDGLDAAREINRRWPGGLRPRIVAMTANAMQGDREQC
                     LAAGMDDYVTKPIRVEALVSALNHVTARPR"
     gene            2299..>6041
                     /locus_tag="VFO53_13150"
     CDS             2299..>6041
                     /locus_tag="VFO53_13150"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET8877272.1"
                     /translation="MASGTGTPVGARSRRAPKAEELAALLALIGRIQQGMLAKLGFQA
                     IVDLVGDTLREMFGSEDLSIRWWDPDADTVVQLYSVEHGVHLPKGPPAKVRATNKPLM
                     KLLYEGVGAYLGTHAEQVAAGTGTAVPGTDWCLSIIGAPIRGAQRVLGIIVIEDHERE
                     HAFGDADLQALTTIGATLGTALENARLFDETQRLLKETEERNAELAVINSIQQAVAAE
                     LGFQAIVDVVGDKLREVFATGDMSIRWWDEATDLVHMLYGYEHGVRLHLPPRVLEPGT
                     IRHRFYHDEREPIVLGSLDEQRARGIEAQPGTDQARSLLVVPVLAGERMLGSVHLENH
                     ERDGAYGPAEVRLLQTIASSMAVALLNARSYEAERQRNAELAVINSIQQGISGSLDFQ
                     GIVDLVGDKLREVLRVKDIGIQWFDVANDRLLILYAYEHGKRLDLAPMKLPESSKRFI
                     RTREPELYRTAAEQIAAGLGAVQGTDQSLSNVVVPIIGSDRVLGLLAMENYEREGAYG
                     EAELRLLQTVAASLGVALESARLFDETQRLLKETGQRNAELAVINSIQQGLASKIELT
                     GIIDLVGDKLRNLFSADLTGIALFDRDRDLMSFPFLYVLGERLRLPSRAQGSRRGIAG
                     LVIRSRQPLVVQSAAEIRTLREQWDDPDVAGVPHDARSLVYMPLLQADHCFGAVVIGK
                     KEEHAFTAADVNLIGTVAASLSVALQNAQSFEAERQRAAELALINTVQRALAGELSLQ
                     GVYDAVGSKLREVFPAMGVSIRRYDAKTGLLHFPFWQTGDEPQRDLPPLKPAGFGGEV
                     LRTRKTLLVNANMEDESRRLGGHSLDKSGRMAKSQLVVPLLMGGEVTGMIDLHDDQKE
                     HSFTDADVRLLETIAATMSVALENARLFDETQRLLKETEARNTELAVVSNIQDGMARA
                     LNFQAIIDLVGDTLRNLFGSSMGIFWLDAPAGLVHFPYAYEHGERLQITPLPLATIAT
                     GRRWYNEILARKTVIWHNRDEYRALELFLVEGTDMSRAGVFAPIVAGDRLLGMLDLEN
                     HDTDNAYGDADVRLLSTVAASMGVALENARLFDETQRLLKETERRSSELAVINSIQQG
                     MAKDLNFQAIVDLVGDKLREMFATGDLAIHWRDERTDVVHHLYVYEHGQRLSPRTSAY
                     KPDARINQALQTGRPVVLGDRAAMDAIGIKTVAGTDDSLSCIFVPVMVGERLIAAISI
                     ESFEREHAFDDAQVHLLSTIAASMGVALENARLLAETQRNARES"
BASE COUNT         1078 a         1981 c         2048 g          934 t
ORIGIN      
        1 cgagctcgag ttcctcgtcg gcctgtcgat ggccgcggcc gtcgcgatgg agaacgcccg
       61 tctcttcgcg caggccgagc agcgcgcgac cgagctcgac acggtgaaca cggtgtcgca
      121 gcaagtggcc gggaagctcg acatctccgc gctgatcgag ctggtcggcg agcaggtgcg
      181 gcgcgtgttc aagccggaca tggcgtacgt cgcgctcctc gaccgcgcga cggaaatgat
      241 tcactttccg tatcgccacg gcgacgtgtt cggttcgagg cggcgtggcg aaggcctcac
      301 gagccagatc atcgacaccg gcgcgccgct gctgctcaac agcgacgaaa gcctcaacgt
      361 ggctgccggc acgaagcgcc tgggcaagca cgcgctgtcg tacctcggcg tgccgatcgt
      421 cgtcgatggc cgcgcagaag gcgtcatcag cgtgcagagc acccagcgcg agggcgtgtt
      481 cgacgcgaac gaccagcggc tgctcgaaac gatcgccgcg agcgtgggcg tggcgctgcg
      541 caacgcgcag cttttcgccg aggcgcgaga ggcgcgcgcc ggggcggaag ccgcgaacga
      601 ggcgaagagc agtttcctcg ccacgatgag ccacgagatc cgcacgccga tgaacgcggt
      661 gatcggcatg agcggcctgc tgctcgacac gccgctttcc gacgagcagc gcgagtacgc
      721 cgcgacgatc cgcgattccg gcgacgcgct cctcacgatc atcaacgaca tcctcgactt
      781 ctcgaagatc gaggccggcc gcatggatat cgaggcgcag ccgttcgatc tgcgcgaatg
      841 cgtcgagtcg gcgctcgatc tcgtgggccc gcgcgccgcg caaaagcagc tcgacctcgc
      901 gtacctcttc gaaggcgacg tgccggctgt cgtcgacggc gacgtcacgc gattgcgcca
      961 gatcctgctc aacctgctgt cgaacgcggt gaagttcacc gagcgcggcg aagtcgtgct
     1021 gacggtcacg ccggatacgc tcgacggcac gcccatgctg accttcgcgg tgcgcgatac
     1081 cggtatcggg ctctcgccgg aagctatcgg ccggctgttc cagaaattca cccaggccga
     1141 tgccagcacg acgcgcaagt acggcggaac gggcctcggg cttgcgatca gcaagcgact
     1201 cgccgagctg atgggcggga cgatgtcggc cgcgagcgac gggccggggc gcggcgccac
     1261 gttcacattc acgatcaacg cgccgcccac cgtgttgcct gcagcgggca ggcgcgagtt
     1321 catcggcgca cagcgcgagc tgcggggccg gcgcgtgctg gtggtcgacg acaatgcgac
     1381 caatcgccgt gtgctgaagc tgcagatggc gaagtggggg atggtgccgg ccgatgccga
     1441 tgcgcccgac gaagcgctgc accgcgttct cgacggacac atgttcgatc tcgcaattct
     1501 cgacatgcac atgccgcaga tgagcggcgt cgagctggcg cggcggttgc gcgcggcgcg
     1561 ccctgccctg ccgctggtgc tgttcacgtc gctcggccgc aaggaggccg gggatgtcga
     1621 cgggctcttc aatgcctatc ttgcgaagcc gcttcgccag agccagctct tcgacacgct
     1681 ggtcacgctc cttgcgcacg acgcgtcgcc gaaggttgca cccgccgcca ggccgcggct
     1741 cgacgccggg atggccgacc ggcatccgct gcgcatcctc gtcgcggaag acaacgtcgt
     1801 gaaccagaag ctcgcgctgc gtcttctgca acagatgggc tatcgcgcag acgtcgcgtc
     1861 gaacggcatc gaagcgatcg agtcgatcga gcgccagccg tatgacatcg tgctgatgga
     1921 tgtgcagatg ccggaaatgg atggactcga cgcggcgcgc gagatcaacc ggcgctggcc
     1981 gggcggtctg cggccgcgga tcgtggcgat gaccgccaac gcgatgcaag gcgatcgcga
     2041 gcagtgcctg gcggcgggaa tggacgatta cgtgaccaag ccgatccgcg tcgaggcatt
     2101 ggtgtcggcg ctcaaccacg tgacggctcg tccgcgctga gatgaatggc cccacgctcg
     2161 ctctgctcgc tgccccccgg gcggctggtc agtcgctcgg ggcggccctt cccgactgac
     2221 atggcgccga gaactgctgt gccgaaagcg aagccgggcg tcaggcgagc cgagcggaag
     2281 aaagccgaag ctccgggcat ggcgtccgga accggcacgc cggtgggcgc gcgatcgcga
     2341 cgcgcgccga aggccgagga gctcgccgct ctgctcgcgc tgatcggccg tatccagcag
     2401 gggatgctcg cgaagctcgg cttccaggcg atcgtggatc tcgtcggcga tacgctgcgc
     2461 gagatgttcg gcagcgagga cttgagcatc cgctggtggg atcccgatgc cgacaccgtc
     2521 gtacagctct acagcgtcga gcacggtgtg cacctgccca aaggaccgcc cgccaaggta
     2581 cgcgccacga acaagccgtt gatgaagctg ctgtacgaag gtgtcggcgc ttatctggga
     2641 acgcacgcgg agcaagtggc ggccggcacc ggcaccgcgg tcccgggaac cgactggtgt
     2701 ctttcgatca tcggcgcgcc gatccgtggg gcgcagcgcg tgctcggcat catcgtgatc
     2761 gaggaccatg agcgcgagca cgctttcggc gacgccgatc tgcaggcact taccacgatc
     2821 ggagccacgc tcggcactgc gctggaaaac gcgcggctgt tcgacgagac gcagcgcctg
     2881 ctgaaagaga ccgaggagcg caatgccgag ctcgcggtga tcaacagcat ccagcaagcg
     2941 gtcgcagccg agctcggctt tcaggcgatc gtcgacgtcg taggcgacaa gctgcgcgag
     3001 gtgttcgcga ccggcgacat gagcattcgc tggtgggacg aagcgaccga tctcgtgcac
     3061 atgctgtacg gctacgagca tggcgttcgc ctgcatctgc cgccgcgagt gctcgagccc
     3121 ggcacgatcc gtcaccgctt ctaccacgac gaacgcgagc ccatcgtgct gggctcgctg
     3181 gacgaacaac gtgcgcgcgg catcgaagcg cagccgggca ccgaccaggc gcgaagcctg
     3241 ctcgtcgtgc cggtgctcgc cggggagcga atgctcggct cggtccacct cgagaatcac
     3301 gaacgcgacg gcgcgtacgg tccggccgag gtgcgattgc tgcagacgat cgcatccagc
     3361 atggccgtcg ccctgctcaa cgcccgcagc tacgaggcgg aacgccagcg caatgcggag
     3421 ctggccgtga tcaacagcat tcagcagggg atatcggggt cgctcgactt ccagggcatc
     3481 gtcgacctcg tcggcgacaa gctgcgcgag gtgctgcgtg tcaaggacat cggcatccaa
     3541 tggttcgacg tggcgaacga tcgcctgctg atcctctatg cctacgagca cggcaagcgg
     3601 ctggatctcg cgccgatgaa actgccggaa tcgtccaagc gcttcatccg cacgcgggag
     3661 cccgagcttt atcgcacggc cgcagaacag attgcggccg gcctcggtgc ggtccagggt
     3721 accgaccaga gcttgtcgaa cgtcgtggtg ccgatcatcg gcagcgaccg cgtgctcggt
     3781 ttactcgcga tggagaacta cgaacgcgag ggcgcgtacg gcgaggccga gctgcgactg
     3841 ctgcagaccg tcgcagcctc gctcggtgtc gcgctcgaga gcgcgcgcct cttcgacgaa
     3901 acgcagcgcc tcctgaagga aaccgggcag cgcaacgccg agttggcggt gatcaacagc
     3961 atccagcagg ggctcgccag caagatcgag ctcacgggca tcatcgacct cgtcggtgac
     4021 aagctgcgca acctgttctc ggccgatctc accggcatcg cgctattcga tcgcgaccgc
     4081 gatctcatgt ccttcccgtt cctgtacgtg ctgggcgagc ggctgcggct tccatcgcga
     4141 gcgcagggta gccggcgcgg cattgccgga ctggtgatcc gcagcaggca gccgctggtg
     4201 gtgcaaagcg ccgcggagat ccggacgttg cgcgagcaat gggacgatcc cgacgtggca
     4261 ggcgtgccgc atgacgcgcg atcgctcgtc tacatgccgc tgctgcaagc tgaccactgc
     4321 ttcggcgccg tcgtgatcgg caagaaggaa gaacacgcgt tcacggccgc cgacgtcaat
     4381 ctgatcggca ccgttgcggc gagcctgagc gtcgcgctgc agaacgcgca gagcttcgag
     4441 gccgagcgcc agcgcgccgc cgagctcgcg ctgatcaata ccgtgcagcg cgcgctggct
     4501 ggagagttga gtctgcaggg cgtctacgac gcggtcggca gcaagttgcg cgaggtcttc
     4561 ccggcgatgg gcgtgtcgat ccgacgctac gacgcgaaga ccgggctgct gcatttcccg
     4621 ttctggcaga ccggtgacga accgcaacgc gacctgccgc cgctgaagcc ggcaggtttc
     4681 ggcggcgagg tccttcgcac gcgcaagacg ctgctcgtca acgcgaacat ggaggatgag
     4741 tcccgccgcc tcggcggcca ctcgctcgac aagagcggac gcatggcgaa gtcgcagctc
     4801 gtcgtgccac tgctgatggg cggcgaagtc accggcatga tcgacctgca cgacgaccag
     4861 aaggagcatt ccttcaccga cgccgacgtg cgcctgctcg aaacgattgc ggcgaccatg
     4921 agcgttgcgc tcgagaacgc gcgccttttc gacgagacgc agcgcctgct gaaggagacc
     4981 gaagcccgca ataccgagct cgcggtcgtc agcaacatcc aggatggcat ggcgagggcg
     5041 ctcaatttcc aggcgatcat cgatctggtc ggcgacacgc tgcgaaatct gttcggctcc
     5101 agcatgggca tcttctggct cgacgcaccg gccgggctcg ttcacttccc ctacgcctac
     5161 gagcatggag agcggctcca gataaccccg ttaccgctcg cgaccatcgc tacgggcagg
     5221 cgctggtaca acgaaattct cgcgcgcaag acggtgatct ggcacaaccg cgacgaatac
     5281 cgggcgttgg agctgtttct ggtcgagggc accgacatga gtcgcgccgg cgtgttcgcg
     5341 ccgatcgttg ccggcgaccg cttgctgggc atgctcgacc tcgagaacca cgacacggac
     5401 aacgcgtacg gcgacgccga cgtgcgcctg ctgtccaccg tcgcggcgag catgggcgtg
     5461 gcgctcgaga acgcgcgcct cttcgacgag acgcagcgtc tgttgaagga gaccgagcgg
     5521 cgctcgtccg agctggcggt catcaacagc atccagcagg ggatggcaaa ggacctcaat
     5581 ttccaggcca tcgtcgatct cgtcggcgac aagctgcgcg agatgttcgc gaccggcgac
     5641 ctcgcgatcc attggcgcga cgagcgcacc gacgtcgtcc accatctcta tgtctacgag
     5701 cacggccagc gactgtcgcc gcgcacctca gcgtacaagc ccgacgccag gatcaatcag
     5761 gcattgcaga ccgggcggcc cgtggtgctg ggcgaccgcg cggcgatgga cgcgatcggt
     5821 atcaagaccg tggcgggcac cgacgacagc ctgtcgtgca tcttcgtgcc ggtcatggtc
     5881 ggcgagcgcc tgattgccgc gatctcgatc gaaagcttcg agcgcgagca cgcgttcgac
     5941 gacgcgcagg tgcatctgct atcgacaatc gcagcgtcga tgggcgtggc gctcgagaac
     6001 gcgcggctgc tcgcggaaac acagcgcaac gcgcgcgaat c
//
LOCUS       DASSVX010000307         6836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_963765, whole genome shotgun sequence.
ACCESSION   DASSVX010000307 DASSVX010000000
VERSION     DASSVX010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963765"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VFO53_13155"
     CDS             <1..824
                     /locus_tag="VFO53_13155"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HET8877273.1"
                     /translation="YDLHCHSTRSDGLLTPSAVVRRAAERGVQVLALTDHDEVAGLDE
                     ARGAARDAGMGFIDGAELSVTWRDTTVHIVALGIDPGNAALAGGLETVRKGRDARARR
                     IGESLAQAGIPDAFDGALAYVTSERLISRTHFARHLIEAGHARDMKDAFKRYLTSGKP
                     GHVPHAWAALTEALNWIHAAGGTAVIAHPGRYKLSAAELRALIADFRDLGGDALEIVS
                     PSHTPGQYDDFAALARRFGLKGSCGTDFHGPGESRFDFGELPPLPAGVDPVWAVW"
     gene            829..1656
                     /locus_tag="VFO53_13160"
     CDS             829..1656
                     /locus_tag="VFO53_13160"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HET8877274.1"
                     /translation="MPDRRTVFFISDRTGITAEMLGNSLLTQFEDIHFHRVTIPFVDS
                     MEKVADAVRQVNATATAEGRRPIVVSSVVDEMMSETIRREVQALTLDMFQIFIQPLEA
                     ELGAKSSHAAGRSHGIANSHEYFARMEAINFTQAYDDGAATRDLDKAQVILVGVSRCG
                     KTPTSLYLALQFGIRAANFPLTPDDFADRRLPGSVVPFRDRLFGLTIQPERLREIREE
                     RRPGSHYAQLDNCRHEVRGAEHLMQQHGIPMLDTTSKSIEEIATTILHRAKLERHIY"
     gene            complement(1675..2454)
                     /locus_tag="VFO53_13165"
     CDS             complement(1675..2454)
                     /locus_tag="VFO53_13165"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET8877275.1"
                     /translation="MRVESRHNERLRDVSRLIASSRDRRKSGRCVLEGVHLVDVYLTR
                     IGVPDTLVVLDDALARPDVAGLVARTPPSRTIVVSRTLFSELATLPADVALLAVVPTP
                     KPPVAAPGQFCLLLEDVQDPGNVGAMIRTAAAAGIDQVLLSRHCAFAWAPKVLRAAQG
                     AHFLTTIVEAVELADWIAGFRRIGGRALATVVAGATPLYAADLRGRVAIVIGSEGAGL
                     SPALLAQVDARIAIPMANGSESLNAAAAAAVVLFEAVRQRS"
     gene            complement(2553..2879)
                     /gene="ftsB"
                     /locus_tag="VFO53_13170"
     CDS             complement(2553..2879)
                     /gene="ftsB"
                     /locus_tag="VFO53_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HET8877276.1"
                     /translation="MRWLALAFVALIVALQYPMWLGKGGWLQVRRYDGDLAAQREANA
                     KLKARNEALDADVRDLKTGYEAIEERARAELGMIRHDEVFFQTQRGGAGMKPAAAPAK
                     KQGAPK"
     gene            complement(2890..4173)
                     /gene="eno"
                     /locus_tag="VFO53_13175"
     CDS             complement(2890..4173)
                     /gene="eno"
                     /locus_tag="VFO53_13175"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET8877277.1"
                     /translation="MTAIVDVIAREILDSRGNPTIEADVVLESGVSGRAAVPSGASTG
                     SKEAIELRDGDKARYFGKGVTKAIENVNTEICEAVLGLDATEQGLIDRTLIDLDGTDN
                     KSRLGANALLAVSCAVAKAAAEECSLPLYRYLGGAGEMQLPVPLMNVINGGAHANNRI
                     DLQEFMLVPLGAPTFREALRYGAEVFHTLKKMIDERGMPTTVGDEGGFAPDLASNESA
                     LALLVEAIDKAGYTPGSDIALALDCAASEFHRDGAYRLESENRALTAKEFADVLATWC
                     DKYPIISIEDGMAEDDWDGWKHLTERLGKRVQLVGDDIFVTNTKILKEGIAKGVANSI
                     LIKINQIGTLSETFAAITMANRAGYTAIVSHRSGETEDSIIADIAVGTNAGQIKTGSL
                     SRSDRIAKYNQLLRIEEDLGDAAAYPGRDAFFNLR"
     gene            complement(4242..5099)
                     /gene="kdsA"
                     /locus_tag="VFO53_13180"
     CDS             complement(4242..5099)
                     /gene="kdsA"
                     /locus_tag="VFO53_13180"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HET8877278.1"
                     /translation="MKLCGYEVGLAQPFFLIAGPCVVESRQLQMDVAGELKAICADLE
                     IPFIFKSSYDKANRSSHASFRGPGMDEGLRILADVRRHVDVPVLTDVHAEAEIGAAAS
                     VVDVLQTPAFLCRQTDFIQAVARAGKPVNIKKGQFLAPEDMRQVVAKARAASQADNIL
                     VCERGASFGYHNLVSDMRALAIMRDTDCPVVFDATHSVQLPGGKGTSSGGQREFVPVL
                     ARAAIAAGVAGLFMETHPNPEEALSDGPNAWPLGQMRALLATLKELDTVVKKAGFPEQ
                     QLMAAGTAR"
     gene            complement(5096..6778)
                     /locus_tag="VFO53_13185"
     CDS             complement(5096..6778)
                     /locus_tag="VFO53_13185"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET8877279.1"
                     /translation="MTKVVFVTGGVVSSLGKGIAAASLAAILASRGVRVTNLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTDVRMTKRNNFTTGQIYESVIRKE
                     RRGVYLGGTVQVIPHITDEIKGWIHSGAGDAEVAVVEVGGTVGDIESLPFLEAIRQMG
                     ITLGRDNVCYIHLTLVPFIPTAGEMKTKPTQHSVKELREIGIQPDVVLCRADRPIPDG
                     DRRKIALFTNVPAEAVIPALDADSIYKIPLALHAEGLDVIVCRKLAIDPPPANLSTWE
                     ALVDALEHPERVVHLAMVGKYVELTESYKSLSEALTHAGIHTRSRVTIHYVDSETIER
                     DGVAALENMDAILVPGGFGKRGVEGKIAAIRYAREHAIPYLGICLGMQLAVIEYARHA
                     ANLPDANSTEFDASTPHPVVALITEWQNRDGTVEKRDASSDLGGTMRLGSQPCDVVPG
                     SLAHRIYGSTSVSERHRHRYEVNNHYLPRIEAAGLTVGAWTKSETAGDLCEMIELPRH
                     PWFFGCQFHPEFTSNPRRGHPLFVSFVRAAIEHRKRAAQSGAAAMPRLVASA"
BASE COUNT         1112 a         2270 c         2276 g         1178 t
ORIGIN      
        1 gctacgacct ccactgccat tcgacccgtt ccgacggact cctgaccccg tcggcggtcg
       61 tgcgccgggc cgccgagcgc ggcgtacagg tgcttgcgct gaccgaccac gacgaagttg
      121 cggggctcga cgaagcccgc ggcgccgcgc gcgacgccgg gatgggattc atcgacggcg
      181 cggagctttc ggtcacctgg cgcgatacca cggtccacat cgtggcgctc ggcatcgatc
      241 ctggcaatgc cgctctcgcg ggcgggctcg agaccgtccg gaagggtcgc gatgcgcgcg
      301 cccggcgcat cggcgaatcg cttgcacagg ccggcatccc cgacgcgttc gacggcgcgc
      361 tcgcgtacgt cacgagcgaa cggctcattt cgcgtaccca tttcgcgcgg cacctgatcg
      421 aggccgggca cgcgcgcgac atgaaggacg cgttcaagcg ctacctgacg agcggcaagc
      481 cgggccacgt gccacacgcg tgggcggcac tcaccgaggc gttgaattgg atccacgcgg
      541 ccggcggcac cgcggtcatc gcgcaccccg ggcgctacaa gttgagcgcg gccgagcttc
      601 gcgcactgat tgccgacttt cgcgacctcg gcggcgacgc gctggaaatc gtttcgccgt
      661 cgcacactcc cgggcagtac gacgacttcg cggcactcgc acgccggttc ggcctcaagg
      721 gatcgtgcgg caccgacttc cacggacccg gcgagagccg cttcgatttc ggggagctgc
      781 ctccgttgcc ggccggggtc gatcccgtgt gggccgtctg gtaaggcgat gcccgaccgg
      841 cgcaccgtct tcttcatctc cgatcgcacg ggcatcaccg ccgagatgct cggcaacagc
      901 ctgctcacgc agttcgagga cattcatttc caccgcgtga cgataccgtt cgtcgattcg
      961 atggaaaagg tggccgatgc cgtgcggcag gtgaacgcga ccgcgacggc cgaaggacgc
     1021 cgtccgatcg tcgtgagctc ggtcgtcgac gagatgatga gcgagacgat ccgccgtgaa
     1081 gtgcaggcgc tgacgctcga catgttccag atcttcatcc agccgctcga agcggagctc
     1141 ggcgcgaaga gctcgcacgc cgccggccgc tcgcacggca tcgcgaacag ccacgaatac
     1201 tttgcgcgga tggaagcgat caacttcacg caggcgtacg acgatggcgc ggcgacgcgc
     1261 gacctcgaca aggcgcaggt gatcctcgtc ggcgtttccc gctgcggcaa gacgccgacg
     1321 tcgctttacc tcgcgctgca attcggcatt cgcgcggcga atttcccgct gacgcccgac
     1381 gatttcgccg atcgccgctt gcctgggtcg gtggttccgt ttcgcgaccg gctgttcgga
     1441 cttacgatcc agcccgagcg gctgcgcgag atccgcgagg agcgccggcc gggaagccac
     1501 tacgcgcagc tcgacaattg ccgccacgaa gtgcgtggcg ccgagcacct gatgcagcag
     1561 catggcattc cgatgctcga cacgacgtcg aagtcgatcg aggagatcgc aacgacgatc
     1621 cttcaccgtg cgaagctcga gcggcatatc tactaggtct gttgccgaca agccctacga
     1681 tcgctgccgc accgcctcga acagcacgac agcggctgca gcggccgcgt tcagcgactc
     1741 gctgccgttc gccatcggaa tggctatgcg cgcgtccacc tgcgccagca acgccggcga
     1801 aaggccggcg ccctcgctgc cgatgactat ggcgacgcgg ccgcgcaggt cggccgcgta
     1861 aagcggcgtc gcccccgcga ccaccgtcgc caacgcgcgt ccgccgatgc gccggaagcc
     1921 ggcgatccaa tcggcaagct cgacggcttc gacgatggtg gtcaggaaat gcgcaccctg
     1981 cgcggcgcgc agcaccttcg gcgcccacgc gaatgcgcag tggcgcgaaa gcagcacctg
     2041 gtcgattccg gcggcagccg ccgtacggat catcgcgccg acgttgcccg gatcctgcac
     2101 gtcctcgagc agcaggcaga actgccccgg cgcggccacc ggcggcttcg gcgtgggaac
     2161 gaccgccagg agcgccacgt cggccggcag cgtcgcgagc tcggaaaaga gagtgcgcga
     2221 cacgacgatc gtccgcgagg gcggtgtgcg cgcgacgagg ccggcgacgt ccggccgcgc
     2281 gagcgcatcg tcgagcacca cgagcgtgtc gggcacgccg atgcgcgtga ggtacacgtc
     2341 gaccagatgg acgccctcga gcacgcagcg gccgctcttg cgccggtcgc gcgacgacgc
     2401 gatcaggcgc gagacgtcgc gcaggcgctc gttatggcgg ctttcgacgc gcatgggtcg
     2461 gagatgacgg ggtcagagac gacttttgct gctcgaacct gatcgaagac gacggccgct
     2521 tcgcgaaagt cgtctctgac cctacgtcgg tgtcacttcg gcgcgccctg cttcttcgcg
     2581 ggcgccgcgg cgggcttcat gccggcgccg ccgcgctggg tctggaagaa cacttcgtca
     2641 tgccggatca tgccgagctc ggcgcgcgcg cgctcctcga tcgcttcgta accggtcttc
     2701 aggtcgcgca catcggcgtc gagcgcctcg ttgcgcgcct tgagcttcgc gttcgcctcg
     2761 cgttgcgcgg cgaggtcacc atcgtaccgg cgcacctgca gccagccccc cttgccgagc
     2821 cacatcgggt attgcagcgc cacgatcagt gcgacgaagg cgagtgcgag ccagcgcatg
     2881 ttttcgtgat cagcgcaggt tgaagaacgc gtcgcgcccc ggatacgcgg ccgcatcccc
     2941 gaggtcttcc tcgatacgca aaagctggtt gtacttcgcg atgcgatccg agcgtgacaa
     3001 cgaaccggtc ttgatctggc ccgcgttggt gcccacggcg atgtcggcga tgatcgaatc
     3061 ctcggtctcg cccgagcggt gcgacacgat tgccgtgtag cccgcgcggt tcgccatcgt
     3121 gatcgccgcg aatgtctccg acagcgttcc gatctggttg atcttgatca ggatcgaatt
     3181 cgcgacgcct ttggcgattc cctccttcag gattttggtg ttcgtgacga aaatgtcgtc
     3241 gccgacgagt tgcacgcgct ttccgagccg ctcggtcagg tgcttccacc cgtcccagtc
     3301 gtcctcggcc atcccgtcct cgatgctgat gatcggatac ttgtcgcacc acgtggcgag
     3361 gacatcggcg aattccttcg cggtgagcgc gcggttctcg gattcgagcc gataggcgcc
     3421 gtcgcgatgg aactcgctcg ccgcgcaatc gagtgccagt gcaatgtccg agcccggcgt
     3481 gtacccggcc ttgtcgatcg cctcgacgag cagcgcaagc gccgactcgt tggatgcgag
     3541 atcgggcgcg aagccgcctt catcgccgac ggtcgtcggc atgccgcgct cgtcgatcat
     3601 cttcttcagc gtatggaaca cttcggcgcc atagcgcaac gcctcgcgga acgtcggcgc
     3661 gcccagcggc accagcatga actcctgcag gtcgatgcgg ttgttcgcgt gcgcacctcc
     3721 gttgatgacg ttcatcagcg gcaccggaag ctgcatctcc cccgcgccac cgaggtaacg
     3781 atagagcggc agcgagcatt cctcggcggc ggccttcgcg actgcgcagg acacggcgag
     3841 cagcgcgttt gcgccaagcc gtgacttgtt gtcggtaccg tcgaggtcga tcagcgtgcg
     3901 gtcgatcagg ccctgctcgg tggcgtcgag gccgagcacc gcttcgcaga tttcggtgtt
     3961 gacgttctcg atcgccttcg tgacgccctt gccgaagtag cgcgccttgt cgccgtcgcg
     4021 cagctcgatt gcttccttcg atccggtgga cgcgccggac ggcacggctg cgcgtccgga
     4081 cacgcccgat tcgaggacca cgtcggcttc aatggtcgga ttgccgcgcg agtcgagaat
     4141 ttcgcgcgcg atgacgtcga cgatggcggt catgttcagg gatcagaggt caggagtcag
     4201 aaagtcagaa gtcagaacgc cgacgcggga aagcgcgcgc atcaccgcgc cgttccggct
     4261 gccatcagct gctgctcggg aaagccggcc ttcttcacga ccgtgtcgag ctccttcagc
     4321 gttgcgagca gcgcgcgcat ttgtccgagg ggccacgcgt tggggccatc ggacagcgct
     4381 tcttcgggat tcgggtgcgt ttccatgaag agaccggcga cgcctgccgc gatcgcggcg
     4441 cgcgccagga cgggcacgaa ttcgcgctgg ccacccgacg acgttccctt gccgcccggc
     4501 aactgcaccg aatgcgtcgc gtcgaagacg accgggcaat cggtgtcgcg catgattgcg
     4561 agcgcgcgca tatcggacac gaggttgtga tagccgaacg atgcgccgcg ctcgcagacg
     4621 aggatattgt cggcctggct cgcggcccgc gccttcgcga ccacctgccg catgtcttcc
     4681 ggcgccagga actggccttt cttgatgttc accggcttgc cggcgcgtgc gacggcctgg
     4741 atgaaatcgg tttgccggca caggaatgcc ggcgtctgca gcacgtcgac caccgatgcg
     4801 gccgccccga tctcggcctc cgcgtgcacg tcggtcagca ccggcacgtc gacgtgcctg
     4861 cgcacgtcgg cgaggatgcg cagcccctcg tccatgccgg ggccgcggaa cgacgcatgc
     4921 gagctgcgat tggccttgtc gtacgacgac ttgaagatga acgggatctc gaggtcggcg
     4981 cagatcgcct tgagctcgcc ggcaacgtcc atctgcaatt gccgggactc gacgacacac
     5041 ggcccggcga tcaggaagaa cggttgtgcg aggccaacct cgtagccaca aagcttcatg
     5101 ccgacgcgac gagcctcggc atcgcggctg cgccgctttg cgccgcgcgc ttccgatgct
     5161 cgatggctgc gcgcacgaag ctcacgaaca gcggatgtcc gcgccgcgga ttcgacgtga
     5221 attcggggtg gaactggcag ccgaagaacc acgggtggcg cggcagctcg atcatttcgc
     5281 acaggtcacc ggccgtttcg ctcttcgtcc aggcgccgac ggtcagtccc gccgcctcga
     5341 tgcgcggcag gtaatgattg ttgacctcgt agcgatgccg gtggcgctcg gaaacgctgg
     5401 tcgagccgta gatgcgatgg gcgagcgacc cgggaacgac gtcgcacggc tgcgagccga
     5461 ggcgcatcgt gccgccgagg tcggagctcg cgtcgcgctt ttcgacggtg ccgtcgcggt
     5521 tctgccattc ggtgatcagt gcgacgaccg gatgcggcgt cgacgcgtcg aactcggtgc
     5581 tgttcgcatc gggcagattc gccgcatggc gagcgtactc gatcaccgcg agctgcatgc
     5641 cgaggcagat gccgagatag ggaatggcgt gctcacgtgc gtagcggatg gcagcgatct
     5701 tgccttcgac tccgcgcttg ccgaagccgc cggggacgag gatggcgtcc atgttctcga
     5761 gcgcggcgac gccgtcgcgc tcgatggttt ccgaatcgac gtaatgaatg gtcacgcgcg
     5821 aacgggtgtg aattccggcg tgcgtcaacg cttcggacag cgacttgtac gattcggtga
     5881 gctcgacgta cttgccgacc atggcgaggt ggacgacgcg ctcgggatgc tcgagggcgt
     5941 ccaccagcgc ttcccacgtc gacaggttgg cgggtggcgg gtcgatcgcg agcttcctgc
     6001 acacgatgac gtcgagtccc tcggcgtgca gcgccagcgg aatcttgtag atcgaatcgg
     6061 cgtcgagcgc cggaatgacc gcttccgcgg gaacgttggt gaagagcgcg atcttgcgcc
     6121 ggtcgccgtc gggaatcggc cggtcggccc ggcacagcac cacatcgggc tggatgccga
     6181 tctcgcgcaa ttccttcacc gagtgctgcg tcggcttggt cttcatctca cccgccgttg
     6241 gaatgaacgg caccagggtc agatggatgt agcagacgtt gtcgcgcccg agcgtgatgc
     6301 ccatctgccg gatcgcctcg aggaatggca gcgactcgat gtcgcccacc gtgccgccga
     6361 cttcgaccac cgcaacctcg gcatcgccgg caccgctgtg gatccatccc ttgatttcgt
     6421 cggtgatgtg cgggatcacc tgcacggtgc caccgaggta aacgccgcgg cgctccttgc
     6481 gaatcacgct ttcgtagatt tgcccggtgg tgaagttgtt gcgcttcgtc atccgcacgt
     6541 cggtgaagcg ctcgtaatgg ccgaggtcga ggtcggtttc ggcgccgtcg tcggtgacga
     6601 acacttcgcc gtgctggaac ggcgacatcg tccccggatc gacgttgatg tacggatcga
     6661 gcttgagatt ggtgacgcgg acgccgcgcg aggcgaggat ggcggcgagc gacgcggcgg
     6721 cgatgccctt ccccaacgag gaaacgacgc cgcccgtcac gaacacgacc ttggtcattg
     6781 gctggccagt tccggaaatt ggtattttac cggaacgccg ccgcggcgcc aaagcg
//
LOCUS       DASSVX010000308         5289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1363821, whole genome shotgun sequence.
ACCESSION   DASSVX010000308 DASSVX010000000
VERSION     DASSVX010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363821"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFO53_13190"
     CDS             complement(<1..332)
                     /locus_tag="VFO53_13190"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696622.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET8877280.1"
                     /translation="MLDSSSQSNIRDAPVRDADLANAIRVLAMDAVEKAKSGHPGMPM
                     GMAEIAVALWQRHLRHNPANPHWPDRDRFVLSNGHGSMLQYALLHLSGYDLPIDELER
                     FRQLHSKTP"
     gene            577..975
                     /gene="speD"
                     /locus_tag="VFO53_13195"
     CDS             577..975
                     /gene="speD"
                     /locus_tag="VFO53_13195"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HET8877281.1"
                     /translation="MYGIHLLGEWYGCPSNEPLLTQADALRDLCRRAVGDAGLTIVGD
                     RFFQFEPQGVTGTVLLAESHLAIHTWPEAGFVTLDVYVCNYTTDNTGKAERVFKALEA
                     ALKPRRKRFQAIHRGAREVVDDEPRRVAAG"
     gene            947..1828
                     /gene="speE"
                     /locus_tag="VFO53_13200"
     CDS             947..1828
                     /gene="speE"
                     /locus_tag="VFO53_13200"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET8877282.1"
                     /translation="MSHDASPPADIAPGLLTEHMTDDWGLFVRSRATLESFRSQYQRV
                     EVHDSVQFGRLFRLDGHFMTSENDEFFYHENLVHLAALAHPGPERALIVGGGDGGSAE
                     ELLKHPSMAQITIAEIDAGVVDISRRHFGEVHRGALDDPRVAVRIEDGFRFVEQAREQ
                     YDLIVLDLTDPGGPSTALYTAEFYEACAARLTPSGAMTMHIASPVAHPSRIRDVLAGL
                     RRAFPRVTPYLTSVPLYGGLWMMACCAATLDPRALSASAVDERIATRRLPLLQYINGD
                     TYRAALALPNFVRDLVG"
     gene            2057..2314
                     /locus_tag="VFO53_13205"
     CDS             2057..2314
                     /locus_tag="VFO53_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877283.1"
                     /translation="MTLKLSETMHDADFVVIDGVVFETAYVRVPDEFTVADDVVLEAR
                     HGETELAFTQADFEDAEHVADGFYRLVDGHLVRFLTSATVH"
     gene            2366..2842
                     /gene="soxY"
                     /locus_tag="VFO53_13210"
     CDS             2366..2842
                     /gene="soxY"
                     /locus_tag="VFO53_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HET8877284.1"
                     /translation="MDTNRRQALKAGSALSLLTLIAAAGWLEPGEAAAAEPWNKRAFD
                     THSLDETMQALGGSAPVPSNDIAFFATPDIAENGAVVPVGITSKVPQTESIAILIEKN
                     PNMLAAVFDIPPGTDASLSTRIKMGQTSSVYALVKANGKYYVTSKEIKVTLGGCGG"
     gene            2866..3177
                     /gene="soxZ"
                     /locus_tag="VFO53_13215"
     CDS             2866..3177
                     /gene="soxZ"
                     /locus_tag="VFO53_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HET8877285.1"
                     /translation="MADPMKIRANLMGDATEVKVLMSHEMETGQRKDADGKVIPAWFI
                     QNVKITHNDKVVLAAQWGPAVAKNPFLSFRFKGGAKGDKVKITWTDNRSDTRTDEATI
                     A"
     gene            3255..4013
                     /gene="soxA"
                     /locus_tag="VFO53_13220"
     CDS             3255..4013
                     /gene="soxA"
                     /locus_tag="VFO53_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HET8877286.1"
                     /translation="MNAALADSTADEIAKYREALQDGNPAELWETRGEDMWKTPRGPK
                     KVSFASCDLGLGPGVVKAAYAQLPRYFPDAKRVMDLETRLVYCMVNQQGVTEADALAN
                     KFGNGSAHRSDLEALAAYVASQSRGVAMNVALDEPHMHDMYRLGEKMFYYRGGTHDFA
                     CATCHGDDGKRIRLQDLPNLTKVEGAQKAYTTWPAYRVSQGEVRTFQWRLNDCFRQQR
                     FPELEFGSDASIALTAYLAGRANGAEYEGPAIKR"
     gene            4028..4669
                     /gene="soxX"
                     /locus_tag="VFO53_13225"
     CDS             4028..4669
                     /gene="soxX"
                     /locus_tag="VFO53_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HET8877287.1"
                     /translation="MRNTLVHRCLAAAAVVTVAAGVLLGCATAPADDGVSDRAAAMMK
                     SSFKARGQATLDRLDQDETQRLCSEYAGRPLPAPIALRIQQANLATIRYPADGKLAGD
                     WKSGEKIAQSGQGFQYSDDPKKPAGANCYACHQLAPQELSYGTIGPSLYRFGKLRGYS
                     EETRRYAWQKVYNAEAFAACSNMPRFGQHHILTEQQIRDVVALLVDPASPVNQ"
     gene            4743..>5289
                     /locus_tag="VFO53_13230"
     CDS             4743..>5289
                     /locus_tag="VFO53_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HET8877288.1"
                     /translation="MQRREFLQALAAGAAAGLRVDAAHALDDTDGERFYAALKPFGNV
                     ALLHITDCHAQLRPTYFREPSVNLGVGDARGKPPHLVGQALLAHFGIAPGTRDAHAFT
                     YLDFAHAARTFGAMGGFAQLATLVKRLRAERPGALLLDGGDTWQGSGTALWTKGQDMV
                     DAAKLLGVDVMTGHWEFTYGAA"
BASE COUNT          954 a         1726 c         1768 g          841 t
ORIGIN      
        1 ggcgttttcg aatgcagctg ccggaatcgc tcgagctcgt cgatgggcag gtcgtaaccg
       61 gacagatgca agagcgcgta ttgcagcatc gacccgtggc cgttcgacag cacgaaacgg
      121 tcgcgatcgg gccagtgcgg attcgccggg ttgtgccgca ggtggcgctg ccacagcgcg
      181 acggcgatct cggccattcc catcggcatg ccgggatgac ccgatttcgc cttctcgacg
      241 gcgtccatcg cgagcacgcg aatggcgttg gcgaggtcgg cgtcgcgcac cggcgcgtca
      301 cggatattcg actggctgga ggaatcgagc atgacgaaac ggcagaaccc gggatctttg
      361 cccggaagac gaaggcaaag ggattgaata cactcgtgga agcaccacta ttgtgccata
      421 attccatgtt ttcggattcg cgcactcgcg cgggagcggt atcgaaatct gcggcggtcg
      481 cccggcttgc ggggcgattt cgccatcttc gcgaaaacac atcggcaacg gttgctccgg
      541 tatccggaag acgcccgcgc gtggaggcta aacgccatgt acggaatcca tctgctgggg
      601 gagtggtacg gatgcccatc caacgagccg ttgctgacgc aggcagacgc attgcgcgat
      661 ctttgtcgcc gtgcggttgg cgacgcgggc ctcacgatcg tcggtgaccg cttcttccag
      721 ttcgagccgc agggcgtcac cggcacggtg ctgcttgccg agtcgcatct cgccatccat
      781 acgtggcccg aggcgggttt cgtcacgctg gacgtgtacg tgtgcaatta caccaccgac
      841 aacacgggca aggccgagcg cgttttcaag gcgctcgaag cggcgttgaa gccgcggcgc
      901 aaacgcttcc aggcaatcca tcgcggggcc agggaggtgg tcgacgatga gccacgacgc
      961 gtcgccgccg gctgacatcg cgccgggact gctgacggag cacatgacgg acgactgggg
     1021 ccttttcgtc cgctcccgcg ccacgctcga atcgttccgc tcgcagtacc agcgtgtcga
     1081 agtgcacgac agcgtgcagt tcgggaggct gttccgcctc gacggccatt tcatgacgtc
     1141 ggagaacgac gagttcttct atcacgagaa tctcgtccac ctcgcagcac tcgcgcatcc
     1201 ggggcccgag cgcgcgctga tcgtgggcgg cggcgatggc ggctcggccg aggagctcct
     1261 caagcatccg tcgatggcac agatcaccat cgcggaaatc gacgccggcg tggtcgacat
     1321 ctcgcgccgc catttcgggg aagtgcatcg cggcgcgctc gacgatccgc gcgttgcggt
     1381 gcgcatcgag gacggcttcc gcttcgtcga gcaagcgcgc gagcagtacg acctgatcgt
     1441 gctcgacctg accgaccccg gcggaccgtc gacggcgctt tacacggccg agttctatga
     1501 agcctgcgcg gcgcggctca cgccgtcggg tgccatgacg atgcacatcg cgagtccggt
     1561 cgcacatcct tcacgcatcc gcgacgtgct cgccggcctg cgccgcgcgt tcccgcgcgt
     1621 cacgccctat ctcacctcgg tgccgctcta tggcgggctg tggatgatgg cgtgctgcgc
     1681 cgcgacgctc gatccgcgcg cgctttcggc ctccgccgtt gacgagcgca tcgcaacgcg
     1741 gcgcctgccg ctgcttcagt acatcaacgg cgacacgtat cgcgcagctc tcgcgctgcc
     1801 gaacttcgtt cgcgacctcg tcggctgaca tcgcgcaggc tcgcgcggcg tgccgcgccc
     1861 gtaggcggaa agccgacgca attccgcggg ttcgtgggct cgacaccctg cggcggggtc
     1921 ggcatcatcg ggctgtcact accggaggtc cgagagtcga aagcccgtct tgaacgtccg
     1981 cctttcgggc cgcatctatc gtttacgaaa agggccgccg cgctgggcgg tcccgccaat
     2041 cgaaggtgac gacgtcatga cgctcaaact gtccgaaacg atgcacgatg ccgatttcgt
     2101 cgtcatcgac ggcgtcgtgt tcgagaccgc atacgttcgg gtccccgacg agttcacggt
     2161 ggccgacgac gtggtgctcg aagcgcgcca cggtgaaacc gagctcgcgt tcacgcaggc
     2221 cgatttcgag gatgccgagc atgtcgccga cggcttttac cggctggtcg acggccatct
     2281 cgtccgcttc ctgaccagcg ccaccgtgca ctgagcgttc gccgattaca atgacgcctt
     2341 ttccgcgcga cgcgggagag gcgcaatgga caccaaccgc aggcaggcgc tcaaagccgg
     2401 aagcgcattg tcgctgctga cgcttatcgc agcggccggc tggctcgagc ccggcgaggc
     2461 cgccgcggcc gaaccctgga acaagcgtgc gttcgatacg cactcgctcg acgaaacaat
     2521 gcaagcgctc ggcggcagcg cgccagtgcc gagcaacgac atcgcgtttt tcgcgacacc
     2581 cgatatcgcc gagaacggcg ccgtcgtgcc ggtcggaatc acgagcaagg tgccgcagac
     2641 cgagtcgatc gcaatcctca tcgagaagaa tccgaatatg ctcgccgcgg tgttcgacat
     2701 cccgccgggg accgacgcat cactgtcgac gcgcatcaag atgggccaga cgtcgagcgt
     2761 gtacgcgctc gtgaaggcca acggcaagta ctacgtgacc tcgaaggaaa tcaaggtgac
     2821 gctcggcggc tgcggcggct gaacgacggg cacgaggaga agacgatggc agacccgatg
     2881 aagatccggg cgaacctcat gggcgatgcg accgaggtca aggtgctgat gagccacgag
     2941 atggaaaccg gccagcgcaa ggatgccgac ggcaaggtga ttccggcgtg gttcatccag
     3001 aacgtcaaga tcacgcacaa cgacaaggtc gtgctcgcgg cgcaatgggg cccggccgtg
     3061 gcgaagaatc cgtttctgtc gttccggttc aagggcggtg cgaagggcga caaggtgaag
     3121 atcacctgga ccgacaatcg cagcgatacg cgcaccgacg aggcgacgat cgcctagaag
     3181 gcagggacag catgcgaacg acgattcagc gcaagctcgc cgccggctgt gccgcgacgg
     3241 cacttgccgc ggtgatgaac gcagcgctgg ccgactcgac ggccgacgag atcgcgaagt
     3301 accgcgaggc gctccaggac ggcaatcccg ccgagttgtg ggagacgcgc ggcgaagaca
     3361 tgtggaaaac gccgcgcggc ccgaaaaaag tctcgttcgc gagctgcgat ctcggactcg
     3421 gacccggtgt cgtcaaggcg gcgtacgcgc aactgccgcg ctatttcccc gatgcgaagc
     3481 gcgtgatgga tctcgaaacg cggctcgtct actgcatggt gaaccagcag ggtgtaaccg
     3541 aagccgacgc gctcgcgaac aagttcggca acggcagtgc gcaccgatcc gacctcgagg
     3601 cgctcgcggc gtacgtcgcg tcgcagtcgc gcggtgttgc gatgaacgtg gcactcgacg
     3661 agccgcacat gcacgacatg tatcggctcg gcgagaagat gttctattac cgtggcggca
     3721 cgcatgattt cgcgtgcgcg acctgccatg gcgacgacgg caagcgcatc cggctgcagg
     3781 acctgccgaa cctcacgaag gtcgagggcg cgcagaaggc gtacaccacg tggccggcgt
     3841 accgcgtgtc gcagggcgag gtgcgaacct tccagtggcg gctcaacgac tgctttcgcc
     3901 agcagcgctt tcccgagctc gaattcgggt ccgacgcgtc gattgcgctg accgcctatc
     3961 tcgccggccg tgcgaatggc gccgagtacg aaggtcccgc gatcaagcgc tgaacggaga
     4021 tccacgcatg cgaaacaccc ttgtccaccg ctgcctcgcg gccgcggccg tcgtcacggt
     4081 tgccgccggc gtgctgctcg gatgtgccac cgcgccggcg gatgacggcg tcagcgatcg
     4141 cgcggcggcg atgatgaaat cgtcgttcaa ggcgcgcggg caggcgacgc tcgatcggct
     4201 cgaccaggac gagacgcagc gtctttgcag cgaatacgcc gggcgtccgc tgccggcacc
     4261 catcgccttg cgcatccagc aggcgaacct cgcgacgatc cgctacccgg ccgacggcaa
     4321 gctggccggc gactggaaga gcggcgagaa gattgcgcag agcggacagg gattccagta
     4381 ttccgatgat ccgaaaaaac ccgcgggtgc gaactgttat gcgtgtcacc agctcgcgcc
     4441 gcaggagctg tcctacggca ccatcggccc atcgctctat cgcttcggca agctgcgcgg
     4501 ctacagcgag gagacgcgca ggtacgcgtg gcagaaggtg tacaacgccg aggcgttcgc
     4561 cgcctgctcg aacatgccgc gcttcgggca acaccacatc ctgaccgagc agcagatccg
     4621 cgacgtggtc gcgctgctcg tggatccggc atcgcccgtc aaccagtgac ggcgcccctc
     4681 accccagccc tctccccgcg tgtcgggaga gggagccgag cgtagcgagg cgggtgaggg
     4741 ggatgcagcg gcgagaattt ttgcaggcgc tcgccgccgg cgctgcggca ggactgcgcg
     4801 tcgacgcggc gcatgcgctc gacgacaccg acggcgagcg cttctacgcg gcgctgaagc
     4861 cgttcggcaa cgtcgcgctg ctgcacatca ccgactgcca cgcgcagctg cgcccgacgt
     4921 acttccgcga gccgagcgtc aacctgggcg tcggcgacgc gcgcggcaag ccgccgcatc
     4981 tcgtcggcca ggcgttgctc gcgcatttcg gcatcgcgcc gggcacgcgc gatgcgcacg
     5041 cgttcacgta tctcgatttc gcgcacgcgg cgcgcacgtt cggcgcgatg ggcggattcg
     5101 cgcagctcgc aacgctcgtg aagcggctgc gcgccgagcg ccccggcgcg ctgctgctcg
     5161 acggtggcga cacgtggcag ggctcgggaa ccgcgctgtg gacgaagggc caggacatgg
     5221 tcgacgccgc gaagctcctc ggtgtcgacg tcatgacggg ccactgggaa ttcacgtacg
     5281 gtgccgcac
//
LOCUS       DASSVX010000309        12540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_563873, whole genome shotgun sequence.
ACCESSION   DASSVX010000309 DASSVX010000000
VERSION     DASSVX010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12540
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563873"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFO53_13235"
     CDS             complement(<1..459)
                     /locus_tag="VFO53_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET8877289.1"
                     /translation="MRTPPSEAGGPLFTGIVEARGRVVACERRADGMRIRVDAGTLDL
                     RDVRIGDSIAVAGCCLTVVELAPPVVHFDVSGETLACTKGFAVNESVNLEKALRLSDR
                     LGGHLMSGHVDGVGTVVALEADYSDAGHRKLAVDVSAALARYVAGKGSVAV"
     gene            complement(478..1587)
                     /gene="ribD"
                     /locus_tag="VFO53_13240"
     CDS             complement(478..1587)
                     /gene="ribD"
                     /locus_tag="VFO53_13240"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET8877290.1"
                     /translation="MTREPFSDEDRRHMVRALALAERGLCTTTPNPRVGCVIVKDGEI
                     IGEGWHQRAGEAHAEVAALADARARGRDTRGATLYATLEPCSRHGRTPPCVDAVLEAQ
                     IGRVVAAMRDPNPAQNGGAERLRGAGVHVDVGLLEAESSALNVGFVSRMMRGRPWVRS
                     KLAASLDGRTALASGESRWITGAAARADGHAWRARACAIVTGVGTVLHDDPQLTVRDM
                     PTTRQPLRIVVDRHADTPANARVLADGNAIVVTAGPRNNEWPSSIETLALPDADGRVD
                     LAALFRALAGRGINEAHVEAGAKLNGALLRAGLIDELLIYFAGALIGDPARGMFESAI
                     PLASLRDRVNVEWTSVERIGDDLRIVARVQMGERA"
     gene            complement(1643..2128)
                     /gene="nrdR"
                     /locus_tag="VFO53_13245"
     CDS             complement(1643..2128)
                     /gene="nrdR"
                     /locus_tag="VFO53_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET8877291.1"
                     /translation="MKCPFCGSDDTQVIDSRVSEAGDSIRRRRKCAACQKRFTTYETV
                     ELRLPQVVKTNGNRADFDTNRLRVGFARALHKRPVPTEFVDAAIDRIVQRVLSLGERE
                     IASRQLGEMVMEELYRLDKVAYIRFASVYRSFQDVSDFRDALREVEAVRPPRKPARRP
                     A"
     gene            complement(2125..3375)
                     /gene="glyA"
                     /locus_tag="VFO53_13250"
     CDS             complement(2125..3375)
                     /gene="glyA"
                     /locus_tag="VFO53_13250"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET8877292.1"
                     /translation="MFSRSTTLAQSDPELWQAISAENRRQEEHIELIASENYASPAVM
                     AAQGTQLTNKYAEGYPGKRYYGGCEHVDVVEQLAIDRVRKLFHAEAANVQPHSGAQAN
                     QAVFFAALSPGDTILGMSLPHGGHLTHGSPVNLSGKWFRVVAYGLDPETELIDYGEAE
                     RLARERKPKLIIAGASAYSRVIEWKRFRAIADEIGALFMVDMAHYAGLIAAGLYPSPV
                     GIADFVTSTTHKTLRGPRGGFILMKEAQAKAINSAVFPGLQGGPLMYVIAAKAVAFAE
                     AMTHEFQVYQERVLENARVLAKVLQERGLRIVSGGTDCHMFLVDLRAKQITGKDAEAA
                     LARAHITVNKNAIPNDPEKPFVTSGIRIGSPAVTTRGFGEIECEELAHLIADVLDAPA
                     DDAALTRTRNSVASLTRRFPVYRA"
     gene            3518..4135
                     /locus_tag="VFO53_13255"
     CDS             3518..4135
                     /locus_tag="VFO53_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HET8877293.1"
                     /translation="MNALLALSRAIDALTERVGRLVYWLVLVVVLISAANATVRKAFD
                     YSSNAYLEIQWYLFSVIFLFGAGYTLLKNDHVRIDIIQGRLSPRAQNWIDVVGIVLFL
                     MPMSLIIMWLSWPLFLDALARHEVSTNAGGLAIWPARLMMPIGFALLIIQAVSELIKR
                     VAFLRGMIPDPLQKRHERSAEEELAVEIARQRGVEAPVLEKGGEP"
     gene            4299..6173
                     /locus_tag="VFO53_13260"
     CDS             4299..6173
                     /locus_tag="VFO53_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET8877294.1"
                     /translation="MTAWLIEYIAPLMFCGLIVTLLIGFPVAFSLGAVGIGFGFLGIH
                     LGLLSTSLLQALPERVFGVMSNDTLLAVPFFTFMGLILERSGMAEDLLDTIGQLFGPL
                     RGGLAYAVVFVGALLAATTGVVAASVISMGLISLPIMLRYGYDRRLATGVIAASGTLA
                     QIIPPSLVLIIMADQLGRSVGDMYAGAFIPGIVLAGIYALYVLATTIVRPQWAPALPV
                     EARTMREANGSSGTPSLIVLVVIAIVASVLYAKLRMAGRALDETLVIATSVGTGVAFL
                     AAVLNRVFRFGLLSRIAERVVFVLIPPLALIFLVLGTIFLGVATPTEGGAMGATGALA
                     MALARRRLTWSLLRQAMDTTAKLSTFVVFILVGARVFSLTFYGVNGHVWVEHLLTSLP
                     GGQLGFLVVVNLLIFVLAFFLDFFELSFIIIPLVGPVADKLGIDLIWFGVLLGVNMQT
                     SFMHPPFGFALFYLRSVAPAKDYVDRVSKKLTKGVTTGQIYWGAVPFVLIQIVMIGLV
                     IAFPQMVLVYKAGETKYDINKIQIIVPEEKPFFPGAPAKGGARTGAQEQDAAERALER
                     AFGGGASEPAPKGETNAPAPAESPPAADEPSPSSEQDEAAKALERAMQGSPPPPGSGK
                     "
     gene            complement(6420..7526)
                     /locus_tag="VFO53_13265"
     CDS             complement(6420..7526)
                     /locus_tag="VFO53_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HET8877295.1"
                     /translation="MERRSFIKHAGLAGILAAGSAPAFAQAAPEVKWRMASSFPRSLD
                     TIFGGADVISKRVAASTNNKFQIQTFAGGEIVPAFGVVDAVQNATIQCAHTAPYYFFG
                     KDPTFAFGCAIPFGMNYRQQNAWMYHGGGMELMREFYKDYNMINFPAGNTGAQMGGFF
                     RKEINKPEDFKGLKMRIGGTGGLPLQKLGIIPQQIPGGDIYPALEKGTIDAAEWVGPY
                     DDEKLGLYKVAKNYYYPGWWEGGPELDLFVNIKAYDELPKDYQSVLQAACYEANVDMM
                     AKYDALNPAALKRLIGGGVKLRPFSNEIMAACYKATVETYDDIATKNAKFKKIYEPWK
                     TFRNDQISWFQVAENRFDNFMIAASRMSQRGPGK"
     gene            complement(7686..9314)
                     /locus_tag="VFO53_13270"
     CDS             complement(7686..9314)
                     /locus_tag="VFO53_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877296.1"
                     /translation="MIDPVLEWLRQLKDPLRNPAQVRRFIARLPERDVLEAQRVSLEL
                     LANFPIGGRDVTAPQLEALLALDARLDPLIADLTRQYTANYQKSSTVESRLWHAVFDL
                     VKGFVGAYYAVLQSGFTHAENRRWRAILPWALVRLAHYRGLDGKFRLFRYSHWIPAQW
                     RDFHETYELARARSWHREQLVFGAGGFSKPGICVEQVYLDMLLLMRLDSGNFTPDQVE
                     WVANQLADWAPTLALTPVASEGAGFYVDLAGAQGLRRRDKPIVGGNVMYVDTGPIYTR
                     IVERLRWLPETDDEKPAPGDLPAREQRLLLMRLAALFGPDALAHAPRASRQSADEHVR
                     VVSGLHALTRAVAEIDRLPDAVRTPGIVASYDEATLINPGINPASIERRVRGTRWKMV
                     DRSESGCRLMAPAKEAPAKLGELLAINDGDNWILGVVRRMQRQQVDEMTVGVEIVARR
                     LVRVLMRSWAAPADVSRAVHDRPFFGIYLPAAPENRQSSQRSLIGPDDRFAPGGMIEL
                     DTGSARYLIRFTQTLERQTGWTWTLFNAVRKLST"
     gene            complement(9353..10744)
                     /gene="pmbA"
                     /locus_tag="VFO53_13275"
     CDS             complement(9353..10744)
                     /gene="pmbA"
                     /locus_tag="VFO53_13275"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HET8877297.1"
                     /translation="MPTSANVEPLATDARAPALARAHFPVTTAALERVAHAVLDAARA
                     GGATAAETEVSQAIGQTVTVRKGEVETIAYNRDKGVGITIYVGARRGHASSADFSDES
                     IRTTVDKALAIARYTAEDPAAGLADPDRLAKSWPDLDLYHPWELPVEDAIELARETEA
                     AALGVDARISNSEGSTVARGESEFVYANSAGFAGGYRSSRHHIDCSVIAEADGAMQRD
                     YWYTAARAPEDLESAQDVGRIAGERTVRRLGGRQLGTLECPVLFEATEAADLLGAFVH
                     AVSGGSLYRKSSFLLDSLGTQVFAPHVNIREEPHLLRARGSAPFDNEGVTTHSRDVVV
                     DGVLCGYFLGSYSARKLGMQSTGNAGGAHNLVVPGTEGLPALLRRMGRGLFVTEQLGQ
                     GVNGVTGDFSRGAAGFWVEDGVIAYPVEEITIAGNLRQMFRDIVALGNDIDRRGSRYI
                     GSVLVSQMTIAGS"
     gene            10801..11325
                     /gene="yjgA"
                     /locus_tag="VFO53_13280"
     CDS             10801..11325
                     /gene="yjgA"
                     /locus_tag="VFO53_13280"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HET8877298.1"
                     /translation="MPRPQLPAPDTAVAEAPSKTRRKLEMHELQDLGVALVALDPRRL
                     ATLELPEALVDAIALARRTNRHEARRRQMQYIGRLMRDVDPAPIRAALAAWGEGSKRD
                     RERFALVERWRDRILDEADGLQAFVAAHPGAPCEALAALAGAARAERTRGAPPAKSRA
                     LFRELRRIVENEPT"
     gene            11312..11878
                     /gene="mog"
                     /locus_tag="VFO53_13285"
     CDS             11312..11878
                     /gene="mog"
                     /locus_tag="VFO53_13285"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HET8877299.1"
                     /translation="MNPPDELVVGLVSISDRASAGIYADQGVPGLTAWFEAALSTPWR
                     FETRLIPDEQPLIEQTLIDLVDTVDCHLVLTTGGTGPAPRDVTPEATLAVAHKVLPGF
                     GEQMRQVSLRYVPTAILSRQVGVVRGRALILNLPGQPKAIKETLDGIFPAVPYCLDLI
                     GGPYVETNDDVCKVFRPKSALRPGTSGT"
     gene            12062..>12540
                     /locus_tag="VFO53_13290"
     CDS             12062..>12540
                     /locus_tag="VFO53_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="HET8877300.1"
                     /translation="MNLKSVIVSTGIGVAFAMAGIAYAQAPAGSTGECKDGTYTTNAT
                     KRGACSGHKGVKDWYGSKNAAPEEKAPKAARTTKAEKAAAESNAGSKSTMAPGAAGAA
                     STMSATPSAPMASSKASTNSAMRTTAAAGGGAGKVWVNSSSNVYHCMNDQWYGKTKQG
                     "
BASE COUNT         1999 a         4280 c         4122 g         2139 t
ORIGIN      
        1 gacggcgacc gagcctttcc ccgcaacgta acgtgcaagc gccgcggaaa catccaccgc
       61 gagtttgcgg tgcccagcgt cgctgtaatc cgcctcgagc gcaaccaccg tcccaacgcc
      121 atcgacgtga ccgctcatca aatgaccgcc caatcggtcg gacaatcgca gcgccttctc
      181 gagatttacg ctttcgttca cggcaaagcc cttcgtgcag gcaagcgttt cgccggagac
      241 gtcgaagtgc accaccggcg gcgctagctc cacgaccgtc aggcagcagc ctgcgacggc
      301 gatgctgtcg ccgatacgga catcgcgaag atcgagcgtt cccgcgtcga cccgaatgcg
      361 cattccgtca gctctgcgct cgcacgcaac cacccggccg cgcgcttcaa cgatgccggt
      421 aaacaacggg cctcccgctt ccgatggcgg cgtgcgcacg atggccggcg gacacgctca
      481 cgcccgctct cccatctgca cgcgcgccac gatgcgcaga tcgtcgccaa tccgctcgac
      541 cgacgtccat tcgacgttca cgcgatcgcg aagtgacgcg agcggaatcg ccgattcgaa
      601 catcccgcgc gcagggtcgc cgatcagcgc acccgcgaaa tagatcagca actcgtcgat
      661 caacccggcg cgcagcagtg cgccgtttaa cttcgccccc gcctcgacgt gcgcttcgtt
      721 gatcccgcga cccgcgagcg cgcggaacag cgcggcgaga tcgacccttc cgtccgcatc
      781 gggcagcgca agcgtttcga tcgacgacgg ccattcattg ttgcgtggtc ccgccgtcac
      841 gacaatcgcg tttccatccg ccagcacacg ggcattcgcc ggcgtgtcgg catgacgatc
      901 gacgacgatg cgcagcggct gccgcgttgt cggcatgtcg cgcaccgtga gttgcggatc
      961 gtcgtgcagc accgtgccga cacccgtcac gatcgcgcat gcgcgcgccc gccatgcatg
     1021 gccatcggca cgtgcggcgg cgccggtgat ccagcggctt tcgccgcttg caagcgccgt
     1081 acgaccgtcg aggctcgccg cgagcttgct gcgcacccag ggccggccgc gcatcatccg
     1141 cgacacgaag cccacgttga gtgccgagga ctcggcctcg agcagtccca catcgacgtg
     1201 cacgccggct ccacgaagtc gctcggctcc gccgttctgc gccggattgg gatcccgcat
     1261 cgccgcgacc acgcggccga tctgcgcttc gagcaccgca tcgacgcacg gcggcgtgcg
     1321 cccgtggcgg ctgcacggct cgagcgtcgc atacagcgtc gcgccgcgtg tatcgcggcc
     1381 ccttgcgcgc gcgtcggcaa gcgccgcgac ttcggcatgc gcctcgccgg cacgctggtg
     1441 ccagccttcg ccgatgattt cgccgtcctt gacgatcacg cagccgacgc gcgggttcgg
     1501 cgtcgtcgtg cacagtccgc gctcggcaag cgcgagcgcg cgcaccatgt ggcggcggtc
     1561 ttcgtcgctg aacggttcgc gcgtcatcga gcttgcttcg tgccacgcaa cggcgtggcc
     1621 gccctcgggg gcgtcgcccc gctcacgccg ggcggcgagc cggcttgcgc ggtggacgca
     1681 cggcctcgac ttccctcagc gcgtcgcgga aatcggatac gtcctgaaac gagcggtaca
     1741 ccgaggcgaa gcggatgtag gccaccttgt cgaggcggta cagctcttcc atcaccatct
     1801 cgcccagttg ccgcgacgca atctcgcgtt cgccgagcga caacacacgc tggacgatgc
     1861 ggtcgatcgc cgcgtcgacg aactcggtgg gcaccggacg cttgtggagc gcgcgcgcaa
     1921 agccgacgcg caaccggttc gtgtcgaaat cggcgcggtt gccattggtc ttcaccacct
     1981 gcggcagccg cagctcgacc gtttcgtacg tcgtgaagcg cttctggcac gccgcgcatt
     2041 tgcgccggcg ccggatcgaa tcgcccgcct cggatacacg cgaatcgatg acctgcgtgt
     2101 cgtcgctacc gcagaacggg catttcatgc ccgatagacc gggaagcggc gcgtgagcga
     2161 cgcgacgctg ttccgcgtgc gcgtgagcgc cgcgtcgtcg gcgggcgcgt cgagcacgtc
     2221 ggcgatcaga tgcgcaagct cctcgcactc gatttcgccg aagccgcgcg tggtcaccgc
     2281 gggacttccg atgcggatgc ccgacgtgac gaacggcttt tccgggtcgt tcggaatcgc
     2341 attcttgttg acggtaatgt gcgcgcgcgc aagcgccgct tcggcgtcct tgccggtgat
     2401 ctgcttggcg cgcaggtcga cgaggaacat gtggcaatcg gtgccgcccg acacgatgcg
     2461 caggccccgc tcctgcagca ccttcgcgag cacgcgcgcg ttctcgagca cacgctcctg
     2521 gtacacctgg aactcgtgcg tcatcgcctc ggcgaatgcg acggccttcg ccgcgatcac
     2581 atacatcagc ggtccgccct gcaggccggg aaagaccgcc gaattgatcg ccttcgcctg
     2641 cgcttccttc atcaggatga agccgccgcg cggcccgcgc agcgtcttgt gcgtggtgct
     2701 ggtgacgaaa tcggcgatgc cgacggggct cggataaagc cccgcggcga tcaggcccgc
     2761 atagtgggcc atgtcgacca tgaagagggc gccgatctcg tcggcgatgg cgcgaaagcg
     2821 cttccattcg atgacgcgcg aatacgccga tgcgccggcg atgatgagct tcggcttgcg
     2881 ctcgcgcgca aggcgctcgg cctctccgta gtcgatgagc tcggtctcgg gatcgagccc
     2941 gtaggcaacc acgcgaaacc acttccccga caggttgacc ggcgagccgt gcgtcaggtg
     3001 cccgccgtgc ggaaggctca tgccgaggat cgtgtcgccg ggcgaaagcg ccgcaaagaa
     3061 caccgcctgg ttcgcctgcg cgcccgaatg cggctgcacg ttcgccgcct cggcgtggaa
     3121 gagcttgcgc acgcgatcga tcgcgagctg ctcgacgacg tcgacgtgct cgcagccgcc
     3181 gtaatagcgc ttgccgggat agccctccgc gtacttgttc gtaagctgcg tgccttgggc
     3241 cgccatcacg gcggggctcg cgtagttttc cgacgcgatg agctcgatgt gctcttcctg
     3301 gcggcgattc tcggcgctga tcgcctgcca cagctcggga tccgactgcg ccagcgtggt
     3361 ggaacggctg aacatggagg gtgcggccgg gaaaacgcaa attctagcat cgcctataat
     3421 tcgcgtccgc ggcccggctg cgcttctccg ccgcggccgt tgcgaacata ctggcaaccg
     3481 aaaaccgggc gcgaccacgc gccacgaggg aggaatcgtg aacgccctgc tcgcgctgtc
     3541 gcgcgcgatc gacgccctca ccgagcgcgt cggccggctc gtctactggc tggtgctggt
     3601 cgtcgtgctg atcagcgccg cgaatgccac ggtccgcaag gcgttcgact acagctcgaa
     3661 cgcgtatctc gagatccagt ggtacctgtt ctcggtgatc ttcctgttcg gcgccggcta
     3721 cacgctgctc aagaacgatc acgtgcgcat cgacatcatc cagggtcgcc tgtcgccgcg
     3781 cgcgcagaac tggatcgacg tggtcggcat cgtgctgttc ctgatgccga tgtcgctgat
     3841 catcatgtgg ctgtcgtggc cgctgttcct cgatgcgctc gcgcgccacg aggtatcgac
     3901 gaacgcgggc ggcctggcga tctggcccgc gcggctcatg atgccgatcg gcttcgcgct
     3961 actcatcatc caggccgtct ccgagctcat caagcgcgtc gccttcctgc gcggcatgat
     4021 tcccgacccg ctgcaaaagc gacacgagcg ctctgccgag gaggagctgg ccgtcgagat
     4081 tgcgcgccag cgcggcgtcg aagcgcccgt gctcgagaag ggcggcgagc cgtgaacggc
     4141 cgccaaagcg ccgcggccga attgacaaat gaggccgcaa gcgtgggggg cccttgagcg
     4201 gcgccgggcc gtcccaagcc gcgaattgct ccccctccgg gggcagcgcc gcggccgaat
     4261 tgaccaatga ggccgcaagc gtggggggac attaaaccat gacggcgtgg ctcatcgaat
     4321 atatcgcgcc gctgatgttc tgcggcctga tcgtcacgct gctgatcggc ttcccggtcg
     4381 cgttctcgct cggcgccgtc ggcatcgggt tcggcttcct gggcatccac ctcgggctcc
     4441 tgagcacatc gctgctgcag gcgctgcccg agcgcgtgtt cggcgtgatg tcgaacgaca
     4501 cgctgctcgc ggtgccgttc ttcacgttca tgggcctgat tctcgagcgc tccggcatgg
     4561 cggaggatct gctcgacacg atcgggcagc tcttcgggcc gttgcgcggc ggtctcgcgt
     4621 acgccgtcgt gttcgtcggc gcgctgctcg ccgccacgac cggcgtcgtc gccgcgagcg
     4681 tcatctcgat ggggctcatc tcgcttccca tcatgctgcg ctacggctac gaccggcgcc
     4741 ttgccaccgg cgtgatcgcc gcgtccggca cgctggcgca gatcattccg ccgtcgctgg
     4801 tgctgatcat catggccgac cagctcggcc gttccgtggg cgacatgtat gcgggcgcct
     4861 tcatcccggg catcgtcctc gccggcatct acgcgcttta tgtgctcgcc acgacgatcg
     4921 tgcggccgca gtgggcaccg gcgctgccgg tggaagcgcg gacgatgcgc gaggcgaacg
     4981 gcagcagcgg aacgccgtcg ctcatcgtgc tcgtggtgat cgcgatcgtc gcttcggtcc
     5041 tgtacgccaa gctgcggatg gcgggtcgcg cgctcgacga gacgctggtc atcgccacca
     5101 gcgtcggcac cggcgtcgcc ttccttgccg cggtcctgaa tcgcgtgttc cggttcggct
     5161 tgctgtcgcg catcgcggag cgggtcgtgt tcgtgctgat ccccccgctc gcgctgatct
     5221 tcctcgtgct cggcacgatc ttcctcggcg tcgcgacgcc gacggaaggc ggcgcgatgg
     5281 gcgcgaccgg tgcgctggcg atggcgcttg cccgccgccg cctgacgtgg agcctgttgc
     5341 ggcaggcgat ggatacgacg gcgaagctgt cgaccttcgt cgtgttcatc ctcgtcggtg
     5401 cgcgcgtgtt cagcctcacc ttctacggag tgaacgggca cgtgtgggtc gagcacctgt
     5461 tgacgagcct gcccggcggc cagctcggct tcctggtcgt cgtcaacctg ctgatcttcg
     5521 tcctcgcgtt cttcctcgac ttcttcgagc tgtcgttcat catcattccg ctcgtcggtc
     5581 ccgtcgccga caagctcggc atcgacctca tctggttcgg cgtgctgctc ggcgtcaaca
     5641 tgcagacgtc gttcatgcac ccgccgttcg gtttcgcgtt gttctacctg cgcagcgtcg
     5701 cgccggcgaa ggactacgtc gaccgcgtgt cgaagaagct gacgaagggc gtcaccaccg
     5761 gccagatcta ctggggcgcc gttcccttcg tgctgatcca gatcgtcatg atcgggctcg
     5821 tgatcgcctt cccgcagatg gttctcgtct acaaggcagg cgagacgaag tacgacatca
     5881 acaagatcca gatcatcgtc cccgaggaaa agccgttctt cccgggcgcg ccggcgaagg
     5941 gtggcgcccg caccggggcg caggagcagg atgcggcgga gcgtgcgctc gagcgcgcat
     6001 tcggcggcgg cgcatcggag ccggcgccca agggcgagac gaacgccccg gcgccggccg
     6061 aaagcccgcc ggcggccgac gaaccctcgc cttcttccga acaggacgag gcggcgaagg
     6121 cgctcgaacg cgcgatgcag ggaagcccgc ctccgccagg cagcggcaag tgaggcgcga
     6181 agatcggggt cagagctgta attcgcgtag gtagtcggaa gatcggggtc agagctgtaa
     6241 ttcgcgtacg cctgttggcg tggcacgcca acgtgaggcg cgaattacag ctctgacccc
     6301 gatcttccca atgtgaggcg cgaattacag ctctgacccc gatcttccga ctaccgccga
     6361 cccaaaagcg aagccccgcg ttgcgcgggg cttcgtgact tcgcgaagga cgacgcgcgc
     6421 tacttgcccg gtccgcgctg cgacatccgg ctcgccgcga tcatgaagtt gtcgaagcgg
     6481 ttctcggcca cctggaacca cgagatctgg tcgttgcgga acgtcttcca cggctcgtag
     6541 atcttcttga acttggcgtt cttggtcgcg atgtcgtcgt acgtttcgac ggtagccttg
     6601 tagcacgccg ccatgatttc gttggagaac ggccgcagct tcacgccgcc gccgatcagg
     6661 cgcttcagcg ccgccggatt caacgcgtcg tacttcgcca tcatgtcgac gttcgcctcg
     6721 tagcacgccg cctgcaggac ggactggtaa tccttcggca gctcgtcgta cgccttgatg
     6781 ttgacgaaga ggtcgagctc cggcccgcct tcccaccagc ccgggtagta ataattcttc
     6841 gccaccttgt acagcccgag cttctcgtcg tcgtagggtc cgacccattc ggccgcatcg
     6901 atcgtgccct tctccagcgc cgggtagatg tcaccgccgg gaatctgttg cggaatgatg
     6961 ccgagcttct gcagcggcag cccgcccgtg ccgccgatgc gcatcttgag gcccttgaag
     7021 tcctcgggct tgttgatttc cttgcggaag aagccgccca tctgggcgcc cgtgttcccc
     7081 gccgggaaat tgatcatgtt gtagtctttg tagaactcgc gcatgagctc catgccgccg
     7141 ccgtgataca tccacgcgtt ctgctggcgg tagttcatgc cgaacgggat ggcgcaaccg
     7201 aacgcgaacg tcgggtcctt gccgaagaag taatacggcg cggtgtgcgc gcactggatg
     7261 gtggcgttct gcacggcgtc gacgacgccg aacgcgggga cgatttctcc acccgcgaat
     7321 gtctggatct ggaacttgtt gttcgtggac gccgcgacgc gcttgctgat cacgtcggca
     7381 ccgccgaaga tcgtgtcgag gctccgcgga aagctggacg ccatgcgcca cttgacttcg
     7441 ggggccgcct gcgcaaaagc cggcgcggac cccgccgcga gtatgccggc cagtccggcg
     7501 tgcttgatga acgaacgacg ctccatcccg tttctcctct cgaatgccgg ccgtgccgac
     7561 ggttggtttg cgagcccgag cccgcgtgcg gactcgctcc ctctccgaag agcggccgtc
     7621 cctcctcggg ggctcggcgc gagcctgtcg attctagcag tccgttcgtt gcggcgtcat
     7681 cgcggctacg tcgacagctt gcgcaccgcg ttgaagagcg tccacgtcca gccggtctgc
     7741 cgctcgagcg tctgcgtgaa ccggatcaga tagcgcgcgc tgcccgtgtc gagctcgatc
     7801 atcccgccgg gtgcgaaccg gtcgtcggga ccgatcaggc tgcgctgcga cgactggcgg
     7861 ttctccggtg ccgcaggcag gtagatgccg aagaacggac ggtcgtgcac cgcgcgcgac
     7921 acatcggccg gcgccgccca gcttcgcatc agcacccgca cgagccggcg cgcgacgatc
     7981 tcgacgccga ccgtcatctc atcgacctgc tggcgttgca tgcgccgcac tacgccaagt
     8041 atccagttgt cgccatcgtt gatcgcgagg agctcgccga gcttcgccgg tgcctccttg
     8101 gcgggcgcca tcaggcggca accgctttcg ctgcggtcga ccatcttcca gcgcgtgccg
     8161 cgcacgcgcc gctcgatcga tgccggattg atgccgggat tgatcagcgt cgcctcgtcg
     8221 tagctcgcaa cgatgcccgg cgtgcgcacc gcatcgggga gccgatcgat ctcggcaacg
     8281 gcgcgcgtca gcgcatgcag accgctcacc acgcgcacgt gctcgtcggc gctctgccgc
     8341 gacgcgcgcg gcgcatgcgc gagcgcgtcg ggaccgaaga gcgcggcaag gcgcatcagc
     8401 agcagccgct gctcgcgtgc aggaaggtcg ccgggcgccg gcttctcgtc gtcggtttcc
     8461 ggcagccacc gcagccgctc gacgatgcgc gtgtagatgg gtccggtgtc gacgtacatc
     8521 acgttgccgc cgacgatcgg cttgtcgcgc ctgcgcagtc cctgcgcgcc ggcgagatcg
     8581 acgtagaagc cggcgccctc ggacgcaacg ggcgtgagcg cgagcgtcgg cgcccagtcg
     8641 gcaagctggt tcgcgaccca ctccacctgg tccggcgtga agttgcccga atcgagccgc
     8701 atcagcagca gcatgtcgag gtagacctgc tcgacgcaga tgcccggctt cgagaatccg
     8761 cccgcgccga acacgagctg ctcgcgatgc cacgaacgcg cacgcgcgag ctcgtaggtc
     8821 tcgtggaaat cgcgccactg cgccggaatc cagtggctgt agcggaagag gcggaacttg
     8881 ccgtcgagcc cgcgatagtg cgcaagacgc accagcgccc acggcaggat tgcgcgccag
     8941 cggcgattct cggcatgcgt gaagccgctc tgcagcaccg cgtaatacgc gccgacgaac
     9001 cccttcacga ggtcgaacac cgcgtgccac agccgcgact cgaccgtcga gcttttctga
     9061 tagttcgccg tgtactggcg cgtgagatcg gcgatcagcg gatcgagcct cgcgtcgagc
     9121 gcgagcagcg cctcgagttg cggcgccgtg acgtcgcgcc cgccgatcgg gaaattcgcg
     9181 agaagctcga gcgacacgcg ctgggcctcg agcacgtcgc gttcgggaag gcgcgcgatg
     9241 aagcggcgca cctgtgcggg gttgcgcagc gggtccttca gctgccgcag ccattcgagg
     9301 acgggatcga tcatcgcgga atcggcatgg gccgcgagcg tccggaaatg ggtcagcttc
     9361 cagcgatcgt catctgcgag acgaggaccg agccgatgta gcgcgagccg cgccggtcga
     9421 tgtcgttgcc gagcgccacg atgtcgcgaa acatctgtcg caggttgccg gcgatcgtga
     9481 tttcctctac gggatacgcg atcacgccgt cctcgaccca gaaccccgcc gcgccgcgcg
     9541 aaaaatcgcc ggtcacgcca ttcacgccct gcccgagctg ctcggtcacg aagaggccgc
     9601 gtcccatccg gcgcaacaat gccggcagac cctcggtgcc cggtacgacg agattgtgcg
     9661 cgccgcccgc attgcccgtc gattgcatgc cgagcttgcg cgccgagtaa ctgccgagaa
     9721 agtagccgca cagcacgccg tcgacgacga cgtcgcgcga gtgcgtcgtc acgccttcgt
     9781 tgtcgaatgg cgcgctgcca cgcgcgcgca gcagatgcgg ctcctcgcga atgttaacgt
     9841 gcggcgcgaa cacctgcgtg ccgagcgaat ccagcaggaa cgacgacttg cgatagaggc
     9901 tgccgccgga aacggcgtgc acgaatgcgc cgagaaggtc cgccgcttcg gtcgcctcga
     9961 acaggacggg gcattcgagc gtgccgagct ggcgcccgcc gagccttcgc accgtgcgct
    10021 cgccggcgat ccggcccacg tcctgcgccg actcgagatc ctcgggcgcg cgcgccgccg
    10081 tgtaccagta atcgcgctgc atggcgccgt cggcttcggc gatcaccgag cagtcgatgt
    10141 ggtgacgcga actgcgatag ccaccggcaa atccggcgct gttcgcataa acgaactccg
    10201 attctccccg cgcgacggtc gagccttcgc tgttcgagat gcgcgcatcg acgccgagtg
    10261 cggcggcttc cgtctcgcgc gcgagctcga tcgcatcctc gaccggcagc tcccacgggt
    10321 ggtagagatc gagatcgggc cacgacttcg cgaggcgatc gggatcggcc aggccggcgg
    10381 cgggatcctc tgccgtgtag cgcgcaatcg cgagggcctt gtcgacggtg gtgcggatcg
    10441 attcgtcgga aaaatccgcc gagctcgcgt gaccgcggcg cgcgccgaca tagatcgtga
    10501 tgcccacgcc cttgtcgcgg ttgtacgcaa tcgtttcgac ctcgcccttg cgcacggtga
    10561 cggtctggcc gatcgcctgc gacacttcgg tttccgccgc ggttgcaccg cctgcgcgcg
    10621 ccgcatcgag cacggcatgc gcgacccgct cgagtgctgc agtcgtgacg gggaaatgcg
    10681 ctcgggcaag cgcaggcgcg cgcgcatcgg ttgccagggg ttcgacgttc gcggaggtgg
    10741 gcatcggaag agggaacatc gggtgtcggc tatcataaca aacgaacctc gctttgcccg
    10801 atgcctcgtc cccaactgcc tgcgcccgac accgctgtcg cggaagcgcc gtcgaagacg
    10861 cgccgcaagc tcgaaatgca cgagttgcag gaccttggcg tggcactcgt ggcgctcgat
    10921 ccgaggcgcc tcgcgacact cgaactgccc gaggcgctcg tcgatgcgat cgcacttgcg
    10981 cgcaggacga accgtcatga agcgcggcgg cggcaaatgc agtacatcgg ccggttgatg
    11041 cgcgatgtcg atcccgcgcc gatccgcgca gcgctcgccg cgtggggcga gggatcgaag
    11101 cgcgaccgcg agcgctttgc gctggtcgaa cgctggcgcg accgcatcct cgatgaggcc
    11161 gacggcctgc aggcgttcgt cgccgcgcat cccggcgcgc cgtgcgaggc acttgcagcg
    11221 ctcgccggtg cggcgcgcgc cgagcgcaca cgcggtgcgc cgcccgcgaa gtcgcgcgcg
    11281 ctctttcgcg aactcaggcg catcgtcgaa aatgaaccca cctgacgaac tcgtcgtcgg
    11341 actcgtgtcg atctccgatc gcgcgtcggc cggcatttac gccgaccagg gcgtgccggg
    11401 actcaccgca tggttcgagg ccgcgctgtc gacgccgtgg cgcttcgaaa cgcggctcat
    11461 tcccgacgag cagccgctga tcgagcaaac gctgatcgac ctcgtcgata cggtcgactg
    11521 ccatctcgtc ctcaccaccg ggggcaccgg gcccgccccg cgcgacgtca cgccggaagc
    11581 gacgcttgcg gtcgcgcaca aggtgctgcc cggattcggc gaacagatgc gccaggtcag
    11641 tttgcgctac gtgccgacgg cgatcctgtc gcggcaggtc ggcgtggtgc gcggccgcgc
    11701 gctgattctc aatcttcccg gacagccgaa ggcgatcaag gagacgctcg acgggatctt
    11761 tcccgcggtg ccctattgcc tcgacctgat cggcggcccg tacgtcgaga cgaacgacga
    11821 cgtgtgcaag gtgttccggc cgaaatcggc gttgcggccg gggacgtcgg gcacgtagcg
    11881 gccgttatcc ggcattcggg cccgttcctc gttaagcacc cgattgtcat tattacaacg
    11941 cgcgtcagcg aatgaacgcg caggaccgcg tcgagtcgaa tcgacttgcc gagggttcac
    12001 gttggtgccg cgtgacccgt cggacctcga tcgagcaggc accgattccg ggaggtcacg
    12061 aatgaatctg aagtcagtca tcgtctcgac gggcatcggc gttgcctttg caatggcggg
    12121 catcgcctat gcgcaagcac ccgccggatc gacgggtgaa tgcaaggacg gcacctatac
    12181 gaccaacgcg acgaagcggg gggcgtgttc cggacacaag ggtgtcaagg actggtacgg
    12241 cagcaagaat gcggcaccgg aggaaaaggc gcccaaggcc gcacgcacga cgaaggccga
    12301 gaaggccgct gccgaatcga atgcgggttc gaagtcgaca atggcgcccg gtgcggccgg
    12361 tgcagcgtcg acgatgagcg ccacgccttc ggcaccgatg gcctcgtcga aggcatcgac
    12421 gaattcggcg atgcgcacga cggcggcggc cggaggcggc gccggcaagg tgtgggtcaa
    12481 ctcgtcgtcg aacgtgtacc actgcatgaa tgaccagtgg tacggcaaga ccaagcaggg
//
LOCUS       DASSVX010000310         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_47177, whole genome shotgun sequence.
ACCESSION   DASSVX010000310 DASSVX010000000
VERSION     DASSVX010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47177"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..618
                     /locus_tag="VFO53_13295"
     CDS             133..618
                     /locus_tag="VFO53_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HET8877301.1"
                     /translation="MPSFDVVSEVNQVEVRNAIDQASKELSTRFDFKGSDARVEYDGK
                     AMTLFADDDFKLSQVTDIVVAKLTKRQVDIRSLKYGDTEKISGNKVKQAVTVRTGVEQ
                     ELAKKVVKLLKESKLKVQASIQGDAVRVSGAKKDDLQAAIALVRKSIADYPLQYQNFR
                     D"
     gene            651..2456
                     /locus_tag="VFO53_13300"
     CDS             651..2456
                     /locus_tag="VFO53_13300"
                     /inference="COORDINATES: protein motif:HMM:NF040132.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877302.1"
                     /translation="MSARNAATALVLACATFPVCCGAFAASLDDASADLCPLTHAGNG
                     APPIYFPVGIAGLERLELAPGFANGIGRVPVSHDEVTRLCSEGFTVHALDTGSVPASF
                     SIVRRSGRVYGNLWNACRTPAAVTTQWLAFDVDAVGYFGQGEHVPIALFLDAHALVAS
                     PPAIVGNGMVIGEVNFAPNGCGSPAFPAAPAGNGEIEAYWRNGNALFGASCGATPLRN
                     AAGYRFVVEADTAAIVHYESSSATTRIASSPPIATAATRPALDTGNAGILVASTNFCS
                     ACADFELHFRDVASGWSAWQIADPRIAQPPFVAPAIGVLAFGAQAQGSASPPRQVVFT
                     NTGATDANVGFAVTADEAPACATDAPRALCDEELDGARRSFVLADSGCHPVRAGGTCV
                     VTVRFEPQMALAIRAHLEYRLADGGITKTVGLEGYGVPPPGAMGSVLAIEYFDPSLGH
                     YFITTLAAEEALLDADAIAGWKRTGAWFTAYRPGEGAAEGAHGVCRYYGRPEAGLDSH
                     FYSASASECDAVAQRFPDAWILESAELFDVALPDPASGECAPDHVPVYRLWNGRASSN
                     HRYTTDASTRRSMIAAGWIAEGYGATGVAMCGAAR"
     gene            complement(2461..>3056)
                     /locus_tag="VFO53_13305"
     CDS             complement(2461..>3056)
                     /locus_tag="VFO53_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HET8877303.1"
                     /translation="TIMPRIERNSLLTLEAYARERPSFRARAVAHKRARTVHVGEHVT
                     LLFEDELTMRYQVQEMLHAEKMFEEAGIQDELDAYNPLIPDGSNLKATMLIEYEDENA
                     RRDALARLKGIEDRVWLRVADLPAVRAIADEDLERENADKTSSVHFLRFELTPPMVDA
                     FSRSGPVTIGVDHPAYQAEVALADPVRKTLAADFAGR"
BASE COUNT          477 a         1030 c         1031 g          518 t
ORIGIN      
        1 acgcatgcca gcttcggcgg tgtcgggccg cgcgagcggc gcgcgcgcgt ggcgccggcg
       61 ccacaccggc ccgcgcggtt tgcctgctcc gacgcccctg tgctagcgtt gagctcccga
      121 tcggagcaag ccatgccctc gttcgacgtc gtgtccgaag tgaatcaggt cgaagtgcgc
      181 aacgccatcg accaggcgag caaggagctc tccacgcgct tcgacttcaa ggggtccgac
      241 gcacgcgtcg agtatgacgg caaggcgatg acgcttttcg ccgacgatga tttcaagctt
      301 tcccaggtca ccgacatcgt cgtcgcaaag ctcacgaagc ggcaggtcga catccgctcg
      361 ctgaagtacg gcgacacgga aaaaatttcc ggcaacaagg tgaagcaggc ggtgaccgtg
      421 cgcacgggcg tcgagcagga gctcgcaaag aaggtcgtca agcttctgaa ggagagcaag
      481 ctcaaggtgc aggcgtcgat ccagggcgac gcggtgcgcg tgtcgggcgc gaaaaaggac
      541 gacctgcaag cggcgatcgc gctggtgcgc aagtcgatcg ccgactaccc gctgcaatac
      601 cagaacttcc gcgactaggc ggttgcgcga tcgagcgacg gttgcccggc atgtccgcgc
      661 gcaacgccgc aacggcgctc gtcctcgcct gcgcgacgtt cccggtgtgt tgcggcgcgt
      721 tcgcggcatc cctcgacgac gcttccgcgg acctctgccc gctgacgcac gcgggcaatg
      781 gcgcgccacc gatctacttc ccggtcggca ttgcgggcct cgagcggctc gagctcgcgc
      841 ccgggttcgc gaacggcatc gggcgcgttc cggtttccca cgacgaagtg acgcggcttt
      901 gcagcgaagg cttcaccgtt cacgcgctcg acaccggctc ggtgcccgcc tcgttctcga
      961 tcgttcgccg ttcgggccgc gtctacggca atctgtggaa tgcgtgtcga acgcccgccg
     1021 ccgtgacgac gcagtggctc gcgttcgacg tggacgcggt cggttatttc ggacagggag
     1081 agcatgtgcc gattgcgctg ttcctcgatg cgcacgcgct cgtcgcaagc ccgcccgcca
     1141 tcgtcggcaa cggcatggtg atcggcgaag tcaatttcgc acccaatggt tgcggcagcc
     1201 ccgcgttccc cgccgcaccc gcgggcaacg gcgagatcga ggcgtattgg cgcaatggaa
     1261 atgcgctgtt tggcgcgagt tgcggcgcga ccccgcttcg caacgccgcc ggctatcggt
     1321 ttgtcgtcga ggcggatacc gcggccatcg ttcactacga gagcagctcg gcgacgacga
     1381 ggatcgcgtc gtctcctccg atcgcgactg cggcgacacg gcctgcgctc gacaccggga
     1441 atgcggggat cctggtcgcg tcgacgaact tctgcagcgc ctgcgccgat ttcgagctgc
     1501 attttcgcga tgtcgcgagc ggctggtcgg cgtggcagat cgccgatccg cgcattgcgc
     1561 aaccgccatt cgtcgccccc gcgatcggcg tcctcgcgtt cggcgcgcag gcgcagggaa
     1621 gcgcgtcgcc gccccggcaa gtcgttttca cgaacaccgg cgcaaccgat gcgaacgtcg
     1681 gcttcgcggt cactgcggat gaggcacccg cctgcgcgac cgatgcgccg cgcgcgcttt
     1741 gcgacgagga gctggacgga gcgcgccgaa gcttcgtgct ggccgattcg ggttgccacc
     1801 cggtccgtgc cggtggcacg tgcgtcgtca ccgtccgctt cgaaccccag atggcgctcg
     1861 ccatccgcgc gcacctcgaa tatcggctcg ccgacggagg catcacgaag acggtcgggc
     1921 tcgagggcta cggcgtgccg ccgcccggcg ccatgggtag cgtgctggcg atcgagtatt
     1981 tcgacccatc gctcgggcac tacttcatca cgacgctggc agccgaagaa gcgctgctcg
     2041 atgcggatgc aatcgccggc tggaaacgta ccggcgcgtg gttcacggcg taccggccgg
     2101 gcgaaggtgc cgcagaaggc gcacacggcg tctgccgata ctacggccgg ccggaagccg
     2161 gtctcgattc gcatttctat tcggcatccg cgagcgagtg cgatgcagtg gcgcaacgct
     2221 tccccgatgc gtggattctc gaatcggccg agctcttcga cgtcgcgttg ccggacccgg
     2281 cgagcggcga atgcgcgccg gaccacgtgc cggtctaccg cctgtggaat ggccgtgcga
     2341 gcagcaacca ccggtatacg accgacgcgt cgacccgtcg aagcatgatc gcggccggct
     2401 ggattgccga gggttatggc gcgaccggtg tcgcgatgtg cggcgccgcg cggtagcggc
     2461 tcagcgtccg gcgaaatcgg ccgcgagcgt cttgcgtacg ggatcggcga gcgcgacctc
     2521 cgcctgatac gccgggtgat cgacgccgat cgtcaccggc ccgctccggc tgaacgcatc
     2581 gaccatcggc ggcgtcagct cgaagcgcag gaaatgcacc gatgacgtct tgtcggcgtt
     2641 ctcgcgctcg aggtcctcgt cggcgatcgc acgcacggcc ggcaggtcgg ccacgcgcag
     2701 ccacacgcga tcctcgatgc ccttgaggcg cgcgagcgcg tcgcggcgcg cattctcgtc
     2761 ctcgtactcg atcagcatgg tcgccttcag gttgctgccg tcgggaatga gtgggttgta
     2821 cgcgtcgagc tcgtcctgga tgcccgcctc ctcgaacatc ttctccgcgt gcagcatttc
     2881 ctggacctgg tagcgcatcg tgagctcgtc ctcgaacagc agcgtgacgt gctcgcccac
     2941 gtgcaccgtg cgcgcgcgct tgtgcgcgac ggcacgtgcg cgaaacgacg ggcgttccct
     3001 cgcgtaggcc tcgagcgtca gcagactgtt gcgttcgatg cgcggcatga tcgttc
//
LOCUS       DASSVX010000311         3487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1213820, whole genome shotgun sequence.
ACCESSION   DASSVX010000311 DASSVX010000000
VERSION     DASSVX010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213820"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1257)
                     /locus_tag="VFO53_13310"
     CDS             complement(61..1257)
                     /locus_tag="VFO53_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HET8877304.1"
                     /translation="MRALIAFLLLAAAAVTLALLFRVNTGYVLFVAPPYRVELSQNAF
                     IIIVVVAFLALYAVIRAAVRVAQMPAEVREGRRRRQFERVRSKQDAAVVALLEGRHGK
                     ARQFAEEALAIPQSSPVAALVGARAALETRDYAGASSMLDRPDTHATNLAVPRLMLEA
                     EIALERGQPAEALARLSELRREAGLHTAAQRLEMRALTAAGRPAEIPALVDQLVKRKV
                     YDPQQGDVLRGSAHAEALASFAHDAAGLRAYWSRVPENDRLQSKVARAAATSFLSLGG
                     DREAADILAKSLERNWDSSLTLLYAECRPADSTKQLETAERWLTRHNQDASLLYALGR
                     LCERQQLWGKAQTYLEASLALDNHWRAHVALGEMLAKLDRNVEADAHFAAALRLALAE
                     LERTIR"
     gene            complement(1313..2407)
                     /locus_tag="VFO53_13315"
     CDS             complement(1313..2407)
                     /locus_tag="VFO53_13315"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HET8877305.1"
                     /translation="MQTTEPDPDRIPGANHSGASTTSPVMRKRVSGATRLLWLVVAIA
                     IAAAAYGWYEQSRITSALRTDVAQRLAAVDAATALARERESDLSNQLRDALAKLALVE
                     TRVAEFQSQQASLEALYRELAPSRDELALSEVEQILVLASQQLSLGSNVQAALAGLQV
                     ADGKLARLDRPQLAPLRRALARDMDRLKAVPYVDVAGISIKLDQLTGTIDSLPLAKDE
                     RLPAPPVAAPAPADTPWLRFVREVWNDLRNVIRIEVSDRPAAPLVTPQQSFFLHENLR
                     LRLLSARLALLSRDERAFKADVQAAIGWLRQYFDLQSKPVQAALAMLAQQQAVAMPGE
                     MPDLTASLDAVRSLKAVADRTPERAAARAK"
     gene            2620..3222
                     /locus_tag="VFO53_13320"
     CDS             2620..3222
                     /locus_tag="VFO53_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877306.1"
                     /translation="MSGFLLIAVIALALAIIVAYRAGARSARNVHAVVTPELRPPVVV
                     DAPDDEARSVAVVQSRNPSIDDSARDTVEPLPSAELAQLREFGDDRRRLLSDLADARA
                     EIARYRQIVIDIESNAPPPLLDAPGTPDDLKMIVGIGPVIERMLYQLGIGTYRQIARW
                     SERDIDDFDARLREFPGRIRRDGWVTQARALHVGKYGSPP"
     gene            3299..>3487
                     /locus_tag="VFO53_13325"
     CDS             3299..>3487
                     /locus_tag="VFO53_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877307.1"
                     /translation="MTVQATTPAARVALILILTLVWGCNWPVLKMGVTELAPLTFRGL
                     TLPLAAVGMLLVAHMSGDS"
BASE COUNT          565 a         1183 c         1141 g          598 t
ORIGIN      
        1 tagccccatc agtaccaagc caagtacgag cagtcgtcgc atcacccctc cgttgtggag
       61 ttagcggatt gtgcgctcga gctccgcgag cgccaaccgc agcgcggcgg cgaagtgcgc
      121 gtcggcctcg acattgcgat cgagctttgc aagcatctcg ccaagcgcca cgtgcgcgcg
      181 ccagtgattg tcgagtgcca agctcgcctc gaggtacgtc tgtgccttgc cccacaattg
      241 ctgccgctcg cacaagcggc caagcgcgta taacagcgac gcgtcctgat tgtgccgcgt
      301 caaccagcgc tcggcggtct cgagttgctt cgtcgagtcc gccggccgac actcggcgta
      361 aagcagtgtg aggctcgagt cccagttccg ttcgaggctc ttggcgagga tgtcggcggc
      421 ttcacgatcg ccgcccagcg acaggaagct cgttgccgca gcgcgcgcta ccttcgactg
      481 cagccgatcg ttttccggca cgcgggacca gtacgcgcga agtcccgcgg catcgtgagc
      541 gaaactcgcc agcgcttccg catgcgcgct gccgcggagc acgtcaccct gctgcggatc
      601 gtaaaccttc cgcttgacga gctgatcgac gagtgcgggg atctcggccg gccgtcccgc
      661 cgctgtcaac gcgcgcattt cgagccgttg cgcggccgta tgcagtcccg cttcgcgccg
      721 aagctccgag agccgcgcga gcgcctcggc gggctggccg cgctcgaggg cgatctccgc
      781 ttcgagcatc agcctcggca ccgcaaggtt cgtcgcatgc gtgtcgggtc gatcgagcat
      841 cgacgaggcg ccggcgtaat cgcgcgtctc gagcgcagcg cgcgcgccga cgagcgctgc
      901 caccggtgac gattgcggga tcgcgagcgc ctcctcggcg aactgccgtg ccttgccgtg
      961 gcggccttcg agcagtgcga cgaccgccgc gtcctgcttg ctgcgaacgc gctcgaactg
     1021 ccgacggcgc cggccctcgc gcacttcggc tggcatctgc gcgacgcgca cagcggcgcg
     1081 tatcaccgcg tacagcgcga gaaacgcgac gacgacaatg atgatgaacg cgttctgcga
     1141 cagctcgacg cgatacggtg gggcgacgaa cagcacgtag ccggtgttga cgcgaaacag
     1201 caacgcgagc gtcaccgccg cggccgcgag cagcagaaaa gcgatcagcg cacgcatcgc
     1261 cggcagctcg acggacggga tgcgttcgcg ccgatcatcg atagaggctc agctacttcg
     1321 cgcgcgcggc ggcgcgttcc ggcgtgcgat cggcaaccgc cttcagcgat cgcacggcgt
     1381 cgaggcttgc cgtcagatcg ggcatctcgc cgggcatcgc caccgcctgc tgctgcgcga
     1441 gcatcgcgag cgctgcctgc accggcttgc tttgcagatc gaaatactgg cgcagccacc
     1501 cgatcgccgc ctggacgtcc gccttgaagg cgcgctcgtc gcgcgacagc aacgcgagcc
     1561 gcgccgacag cagtcgcaat cgcaggttct cgtggaggaa aaacgactgt tgcggcgtga
     1621 cgagcggcgc cgcgggacgg tccgacacct cgatgcggat cacgttgcgc aggtcgttcc
     1681 acacttcgcg tacgaagcgc agccatggcg tatcggccgg tgcgggtgct gcgaccggcg
     1741 gcgccggcag ccgctcgtcc tttgcaagcg gcagcgagtc gatcgtgccg gtcaactggt
     1801 cgagcttgat cgagatgccg gcgacatcga cataagggac ggctttcagg cgatccatgt
     1861 cgcgcgccag tgcgcggcgc agcggcgcga gttgcggacg atcgagacgc gcgagcttgc
     1921 cgtcggcgac ctgcagaccg gcgagcgctg cctgcacgtt gctgccaagc gaaagctgct
     1981 ggcttgcgag caccagaatc tgctcgacct cggatagcgc aagctcatcc cgcgatggcg
     2041 cgagctcgcg atacagtgct tcgagcgacg cctgctgcga ctggaactcg gcgacccgcg
     2101 tctcgaccaa cgcaagcttc gcgagcgcgt cgcgcaactg gttcgacaag tccgattcac
     2161 gctcgcgcgc gagtgccgtt gcggcatcga cggcggcgag ccgctgcgca acgtccgtgc
     2221 gcagtgcgct ggtaattcgc gattgctcgt accagccgta ggcggcggca gcgatggcga
     2281 tcgcgaccac cagccacaaa aggcgtgtcg cgccgctgac ccgcttacgc atcaccggtg
     2341 acgtcgtcga tgcaccgctg tggttagcac cgggaatgcg atcgggatcg ggttcggtgg
     2401 tctgcatgaa ggcacttgac cgaattttat cagtcgccga tcacttcccc tgcgcgcgca
     2461 cctggagcac gcgcgcgccg tcgaatacaa tgccggggct ttcttcggct cgcctcgcgc
     2521 atcccatccc gaacagcgcc ccgtcgcgat tggcatagtt atgcagaggc tccctagcgc
     2581 catgcatcgt tgccgcattc ccgccgagcg acgccgtccg tgagcggctt tctgctcatc
     2641 gcggtgattg cgctggcgct tgcgatcatc gtcgcctatc gcgctggcgc gagatcggct
     2701 cgcaatgtgc acgccgttgt tactcccgag ttgcgcccgc cggtcgttgt ggatgcgccg
     2761 gacgacgagg cgcgatctgt ggccgtcgtc cagtcgcgta atccatcgat cgacgactcg
     2821 gcccgagaca ccgttgaacc gttgccgagc gccgagctcg cgcagctgcg ggaattcggc
     2881 gacgatcggc gcagattgct gtcggacctt gccgatgcgc gtgcggaaat cgcccgttac
     2941 cggcagatcg tcatcgacat cgagtccaat gcgcctccac cactcctcga tgcgcccggc
     3001 acgccggatg acctcaagat gatcgtcgga atcggcccgg tgatcgagcg gatgttgtat
     3061 cagcttggca ttggcacgta ccggcagatc gcgcgctgga gcgagcgcga catcgacgac
     3121 ttcgatgcac gactgcgcga gtttccgggt cgcattcggc gcgatggctg ggtgacgcag
     3181 gcgcgcgcgc ttcacgtcgg caaatacggc agtccgccgt agcgaatcac tcgagtgtcc
     3241 cgacggctgc ttgcgagcag ctgtttcggt gcccgtctcc cattcaaacg actcgtcgat
     3301 gaccgttcaa gccaccaccc ccgccgcgcg cgttgcgctg atcctaatcc tcacactcgt
     3361 ctggggctgc aactggccgg tgctgaagat gggggtgacc gagctcgccc cgctgacatt
     3421 tcgcggcttg acgttgccgc tcgcggccgt cggcatgctg ctcgtcgcgc acatgtcggg
     3481 cgactcg
//
LOCUS       DASSVX010000312         7827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_580788, whole genome shotgun sequence.
ACCESSION   DASSVX010000312 DASSVX010000000
VERSION     DASSVX010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580788"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFO53_13330"
     CDS             <1..389
                     /locus_tag="VFO53_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877308.1"
                     /translation="LRKNRIANETMLKLAERGVVPPATAMEAVASGTAASVAAAAVPP
                     PGNPAYAQARYLQRRTACSDLRKGIILTAVGLGLSFWSMLDDGTPNSVGLVLLFVGLG
                     YCLLWFFEDRTMAPPRPPGTPPPGSV"
     gene            508..1110
                     /locus_tag="VFO53_13335"
     CDS             508..1110
                     /locus_tag="VFO53_13335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET8877309.1"
                     /translation="MAAAARSHDVTDAQLIARCIVADDRHAFAELVKRHQSSVRACLR
                     KLTAGNHALADDLAQDTFVLAWRNLKSFRQEARFSTWLYRIATNCWLAQARKRHEELL
                     GDRDADVSDDDAGDATLPGGAGGDHAAAATMKIDLERALARLSEAERAAIVQCYHNDL
                     SHEEAAYVLGWPVGTVKTHILRGKQKLRAALAAWAPPAAT"
     gene            1116..1481
                     /locus_tag="VFO53_13340"
     CDS             1116..1481
                     /locus_tag="VFO53_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877310.1"
                     /translation="MVTSSNPAPPASADWLDALLVSAAADHRALYVTDEGFSARVVQR
                     LPARDALPAWRRPIVAGLWLVAAALIATMLPGTAHEIAREVFTLFAARPFSLSTLGFA
                     IVAVGIATWTGAALALRRD"
     gene            complement(1563..2222)
                     /locus_tag="VFO53_13345"
     CDS             complement(1563..2222)
                     /locus_tag="VFO53_13345"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET8877311.1"
                     /translation="MDFARQQRDPTRHMIGIAFVVLFHALIIWALVTGLGQKVIQVVK
                     KPLTAQIIEEIKAPPPPPPPPPPPPKRIEPPKPVIEQPYVPPPEIPPPVMAPPPVIAN
                     VAPTPPPVPVVIAPPAPPVVIAPPAPPKPAIRQGVSCTKRDPPSFPREAIRAHVEKGH
                     VDAVLTIDEKGNVSDVQIVTSSPPRVFDRVVRDTLAEWKCAGEGAKYKASVEINFTLT
                     D"
     gene            complement(2251..2682)
                     /locus_tag="VFO53_13350"
     CDS             complement(2251..2682)
                     /locus_tag="VFO53_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET8877312.1"
                     /translation="MAMNVAAPSRSSDPDVMIDINTTPLIDVMLVLLVMLIITIPIQL
                     HAVNLNLPIGNPPPPLTLPEVVKIDVDDQSKVYWNGEVLPDRATLESKISEAAKQSVQ
                     PEVHLRPNKLAKYSVVAGVMASAQRLGLTKIGIVGSEQFVE"
     gene            complement(2717..3157)
                     /locus_tag="VFO53_13355"
     CDS             complement(2717..3157)
                     /locus_tag="VFO53_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET8877313.1"
                     /translation="MAMNVGTADGDDDEVMSTINTTPLVDVMLVLLIIFLITVPVVRN
                     SIPVELPKERNEIRESKPENIVISVDSQDHIYWNDLRMASTQALVDRLKKISVLSPQP
                     EVQIRGDGRGNYEGVGRVVYACQRAGISKVSFITEPPPRAAAAS"
     gene            complement(3183..4049)
                     /locus_tag="VFO53_13360"
     CDS             complement(3183..4049)
                     /locus_tag="VFO53_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET8877314.1"
                     /translation="MNRLANLAAALMLAMPIAATPLSAQAQAQTTTPPSAAPADAPVA
                     TPAPAPAAPAAAAKAAPATTDVLENPYGLEALWKGGDLVARITLGILVVMSAGSWYII
                     VTKVYEQAKMNRQARAADRSFWKAASVQKGAVELREGSPYRFIAESGLEATTKHEGLL
                     GNVDMNTWVSMSIQRAIDNIQSRTQDGLAFLATVGSTAPFVGLFGTVWGIYHALTAIG
                     IAGQASIDKVAGPVGEALIMTAIGLAVAVPAVLGYNWLIRRNKAAMERVRGFGADLHA
                     VLLSATQSPSRA"
     gene            4548..4820
                     /locus_tag="VFO53_13365"
     CDS             4548..4820
                     /locus_tag="VFO53_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877315.1"
                     /translation="MNRYDPRTPRTLFAVAAVAMTAATLAISVLAPAGVERPAPQDDL
                     LTRVESETCAPVGNMLVTAIDVVAIRAPHVAPVAQSHGAAQRLARS"
     gene            complement(4840..5349)
                     /locus_tag="VFO53_13370"
     CDS             complement(4840..5349)
                     /locus_tag="VFO53_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877316.1"
                     /translation="MPTILRYLSNRVVNPLVVAVFLGIALVSVHAHAQDKSADATDIQ
                     VLRDALKTDKRAYIASMLSLTSAEAKRFWPIYDSYQRVIDETSQRRVVALKDLVMGDS
                     PTTNLAAKNVMLELVNIYDTEAKAHLRLARRVMRALPAIKAARYLQLEDKMLAVRDYD
                     TASAVPLMH"
     gene            complement(5441..7645)
                     /locus_tag="VFO53_13375"
     CDS             complement(5441..7645)
                     /locus_tag="VFO53_13375"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8877317.1"
                     /translation="MRSSLAKALSDAAGHHAHGRLSEAEAGYRSVLQNAPDQPDALHR
                     LGMIAHQVGRNEAALELIDRAIMVNPVQPTYYNDAGIVLQALGRNDEAIARFRQALAL
                     AHAYPRAHNGLGIALHDQGRLDEAIASFRTALSLKPDYADAQNNLANVLREQGRAAEA
                     VQIYRKALETRDVPDVRYNLASALSDLGAFDDAAQAYAKALIRRETPDAIAGFVQSLV
                     AAELRHADDDVRRMALRALAAPWARPSELSAACIRLLASGAAMRECIDRAWIAWPARV
                     TLGDLLGGVDPGVVFDDALLRALLESAPVCDLSMERFLTLARFALLDAAIESRHPAND
                     DRLAFCCALARQCFIDDYVFACTGTEIERASALRNVVAASLERGEAVAPVPVAIVAAY
                     FPLISMPQREALLARTWPEPLDALLTQQLREPLEERGLRDRLRRLTEIDDRVSIDVRR
                     QYEEHPYPSWVRLPSPAPTSPAGAGPGGRFPLELRQSGERDLDILVAGCGTGQESIEL
                     AYAFPRARVLAIDLSLASLAYAERKARELYVANLEHAQADIMNVAALGRRFDVISSVG
                     VLHHLADPVAGWRELVAMLAPGGHMLVGLYSERSRQDVVAARAFIAAAGYLPTDTDIR
                     RCRQDLLSSDHAVQIDRLTRRSDFYVTGECRDLLFHVQEHRFTLPRIAEAIDALGLRF
                     NGFLLGEDLARRYAEHYPDDPGMENLDNWDRLEAQLPQAFAGMYIFWVQKAR"
BASE COUNT         1282 a         2656 c         2608 g         1281 t
ORIGIN      
        1 agctgcgcaa gaaccgcatt gcgaacgaga cgatgctgaa gctcgccgag cgcggcgtcg
       61 tgccgcctgc cacggcaatg gaagcggtcg cctccgggac ggccgcgtcc gtcgcggccg
      121 ccgccgtgcc gccgccgggc aatcccgcct acgcgcaggc gcggtacctg cagcggcgca
      181 cggcctgctc cgatctgcgc aagggcatca tcctcaccgc ggtcggcctc ggcttgagct
      241 tctggtcgat gctcgacgac gggacgccga acagcgtcgg cctcgtcctg ctgttcgtgg
      301 gccttggcta ttgcctgttg tggttcttcg aggaccgcac gatggcgccg ccgcgtccgc
      361 cggggacacc cccgccgggg agcgtgtgat gtctcgtcgc tcgatcatcc gctgtgctgc
      421 gggccgccat gcatgaaccg gccggcgacc acgcccgctc cgcgccgcgg ccgcgcgcgg
      481 gtcgcacccg cgcatcgcgc ttgacggatg gctgcagcag cccgatcgca cgacgtcacc
      541 gacgcgcaac tgatcgcgcg ctgcatcgtc gcggacgacc ggcatgcgtt cgccgagctc
      601 gtgaagcgtc accaatcgtc ggtgcgcgca tgcctgcgca agctgaccgc cggcaatcac
      661 gcgctggccg acgatctcgc gcaggacacg ttcgtcctcg cgtggcgcaa cctcaagtca
      721 ttccgccagg aggcgcgctt ctcgacgtgg ctctaccgca tcgcgaccaa ttgctggctt
      781 gcgcaggcgc gaaagcggca cgaggaattg ctcggtgacc gcgatgcgga cgtcagcgac
      841 gacgatgccg gcgacgctac gctgccgggg ggtgccggcg gcgatcacgc ggcggccgca
      901 acgatgaaaa tcgacctcga gcgtgcgctc gcgcggctgt ccgaggccga gcgtgcggcc
      961 atcgtccagt gctaccataa tgacttgtcc cacgaagagg ccgcgtacgt gctgggctgg
     1021 ccggtgggta ccgtgaaaac gcacattctt cgcggcaagc agaagcttcg ggcggcgctc
     1081 gccgcctggg cgccgccggc agcgacgtaa acgccatggt cacctcgtcg aacccggctc
     1141 cgccggcaag cgccgattgg ctcgatgcgc tgctcgtcag cgccgcggcc gaccatcgcg
     1201 ccctgtacgt caccgacgaa ggcttcagcg cacgcgtcgt gcaacgtctg ccggcgcgcg
     1261 acgcgcttcc cgcgtggcgc aggccgatcg tcgcgggcct ctggctcgtc gccgcagcac
     1321 tcatcgcgac gatgctgccc ggaaccgcgc atgagatcgc gcgcgaagtg ttcacgctgt
     1381 tcgcggcgcg gccgttttcg ctgtcgacgc tcggattcgc gattgtcgcg gtcgggatcg
     1441 cgacgtggac gggggccgcg ctcgcgttgc gcagagattg agcgtccgaa acgcgcgttg
     1501 ccggacccga cgaaagtatc gcgcgcgcca tcgacgcacg cgaaacgttc gtacgccagc
     1561 gcctaatcgg tcaacgtgaa gttgatctcg acggaggcct tgtactttgc gccttcgccg
     1621 gcgcacttcc actcggccag cgtgtcgcgc acgacgcggt cgaacacgcg cggcggcgat
     1681 gacgttacga tctgcacgtc cgacacattg cccttttcgt cgatcgtcag gacggcgtcg
     1741 acgtgaccct tctccacgtg cgcgcggatg gcctcgcgcg ggaacgacgg cggatcacgc
     1801 ttggtgcatg aaacgccctg ccggatggcc ggcttgggcg gcgccggcgg cgcaatcacg
     1861 acgggcggcg cgggcggcgc gatgacgacg ggcaccggcg gcggcgtcgg cgccacgttg
     1921 gcgatgaccg gcggcggcgc catcaccggc ggcggtatct ccggcggtgg gacgtacggc
     1981 tgctcgatca ccggcttcgg cggctcgatc ctctttggcg gcggcggcgg tggcggtggc
     2041 ggcggcggcg gggccttgat ttcctcgatg atctgcgccg tcaacggctt cttgacgacc
     2101 tggatcacct tctggccgag accggtcacc agcgcccaga tgatgagcgc atgaaagagg
     2161 accacgaacg cgatcccgat catgtgccgc gtcgggtcgc gttgctggcg tgcgaaatcc
     2221 attggcctgt tcgactcctg taggcgtccg ctactcgacg aactgctcgc tgcccacgat
     2281 gccgatcttg gtcaggccga gccgctgcgc cgacgccatg acgccggcaa ccaccgagta
     2341 cttggcaagc ttgttcggcc gcagatgcac ctcgggctgc accgactgct tcgcagcctc
     2401 gctgatcttc gattcgagcg ttgcgcgatc gggcagcact tcgccgttcc agtacacctt
     2461 gctctggtcg tcgacgtcga tcttcacgac ttcgggcagc gtgagcggcg gcggcggatt
     2521 gccgatcggc agattgagat tgaccgcatg cagctggatc ggaatcgtga tgatcagcat
     2581 cacgagcagc accagcatca cgtcgatcag cggcgtcgtg ttgatgtcga tcatcacgtc
     2641 ggggtcggac gagcggctgg gcgcggctac gttcatcgcc atggcaggaa tcctcggatg
     2701 atttcgaccg cgtgcatcag cttgccgccg ccgcgcgcgg cggcggctcg gtaatgaacg
     2761 acactttcga gatgccggcg cgctggcacg catacaccac gcgcccgaca ccttcgtagt
     2821 tcccgcgccc gtcgccgcga atctgcacct cgggctgcgg cgaaagcacc gaaatcttct
     2881 tcagccggtc gacgagcgcc tgcgtcgacg ccatccgcag gtcgttccag tagatgtggt
     2941 cctgcgaatc gaccgagatc acgatgttct cgggcttcga ttcgcggatc tcgttgcgct
     3001 ccttcggcaa ctcgaccggg atcgagttgc gcacgacggg caccgtgatg aggaagatga
     3061 tcagcaggac cagcatgacg tcgaccagcg gcgtcgtgtt gatcgtcgac atcacctcgt
     3121 cgtcgtcgcc gtcggcggtc cctacgttca ttgccatggc gttgcgtccc ttcgcgtgcg
     3181 ggtcaggccc tggacggcga ttgtgtcgcc gacagcagca ccgcatgcag gtcggcgcca
     3241 aagccgcgga cgcgctccat ggcggccttg ttgcggcgga tcagccagtt gtagccgagc
     3301 accgccggga ccgcaaccgc gagaccgatc gccgtcatga tcagcgcctc gccgaccgga
     3361 ccggccacct tgtcgatcga cgcctggccc gcgatgccga tggccgtcag cgcgtgatag
     3421 atgccccaca cggtgccgaa caggccgacg aacggggccg tcgaacccac ggtggcgagg
     3481 aacgcgaggc cgtcctgcgt gcggctctgg atgttgtcga tcgcgcgctg gatcgacatg
     3541 ctcacccagg tattcatgtc gacgttgccg agcaggccct cgtgcttggt ggtggcctcg
     3601 aggcccgatt cggcaatgaa ccggtagggg cttccctcgc gcaactcgac ggcgcccttc
     3661 tgcaccgacg ccgctttcca gaacgagcgg tcggcggcgc gcgcctggcg attcatcttc
     3721 gcctgctcgt ataccttggt gacgatgatg taccagctcc ccgcggacat cacgacgagg
     3781 atgccgagcg tgatgcgcgc gacgagatcg ccgcccttcc acaacgcctc gaggccgtaa
     3841 ggattctcga gcacgtcggt ggtcgccggc gcggccttgg cggcggcggc cggcgccgcg
     3901 ggtgcaggtg cgggggttgc gacaggtgca tcggccggag cggcgctcgg cggcgtcgtc
     3961 gtttgcgctt gcgcctgtgc cgaaagcggg gtcgccgcga tcggcattgc aagcatcagg
     4021 gccgcggcga ggttcgccag acggttcatc ttcaacatgt tgtctccatg cgaatgttgg
     4081 gcaacgaact tccgggtatc cagacgatgc gcacggcgcg cggtcccgaa tcgcgacgcg
     4141 aaggctcgag gcgcgcggta ccagcgttcc ctgcacggtt ggcaggtgca tccggccccg
     4201 gcgccgcgat gctgagctgc ggcccaaaac cacatggggg tgcacccgaa aaacatccgg
     4261 gccccgccgg aacgggacca gacaacgtcg gaaaatcgcg cgattctaca caatacggcg
     4321 aggaagtcca cccggacgcg tcacgcaccc ggcgccattc gtcgcgccgg ccgtactctc
     4381 ggccgcgtaa ttcgcggttc cgtcgcatcc gggttgccgc gccgcgcgag catcgccacg
     4441 atgcactcca tgcacggttg cccgccgcga tccgaatcat gaatccggtg cgccgcgaag
     4501 atgcatcgaa ccgcagagtt cgcacgatcg acaacatgga gccgcagatg aaccgctatg
     4561 atccccgcac gccccgcacg ctgttcgcag tcgccgccgt cgcgatgacc gccgccaccc
     4621 tcgccatttc ggtgctcgcg cccgcgggag tcgagcgccc ggcgccgcag gacgatctcc
     4681 tcacccgcgt ggaaagcgag acctgcgctc cggtcggcaa catgctcgtc accgcaatcg
     4741 acgtcgtcgc cattcgcgcg ccgcatgtcg cgccggttgc gcaatcgcac ggcgccgcgc
     4801 agcgcctcgc gcgaagctga gacatgtgcg cgatgcgggt cagtgcatca gcgggaccgc
     4861 cgacgccgtg tcgtaatcgc gcaccgcgag catcttgtcc tcgagctgca gatagcgcgc
     4921 cgccttgatc gccggcagcg cgcgcatcac gcgacgcgca aggcgcaggt gcgccttcgc
     4981 ttcggtgtcg tagatgttca cgagctccag catcacgttc ttggcggcga ggttcgtcgt
     5041 tgggctgtcc cccatcacga gatccttgag ggcgacgacc cgtcgctggc tcgtctcgtc
     5101 gatgacccgc tggtagctgt cgtagatcgg ccagaatcgc ttggcctcgg cgctcgtcag
     5161 cgacagcatc gatgcgatgt aggcgcgctt gtcggtcttc agcgcatcgc gcagcacctg
     5221 gatgtccgtg gcgtccgcac ttttgtcctg cgcatgcgca tgcaccgaca cgagcgcgat
     5281 gccgaggaaa acggcgacca cgagcggatt cacgacacga ttgctcaaat aacgcagaat
     5341 cgttggcacg cgagactcct tgaatgacgg acacaatgcg gacggataac gcgaacggct
     5401 gaaacgagct cccgcgcggc cggaacggta accgcgcggc tcatcgcgcc ttctgcaccc
     5461 agaatatata catgcccgcg aaggcctgcg gtagctgcgc ttcgagccga tcccagttgt
     5521 cgagattctc catgcccgga tcgtccggat agtgctccgc atagcgccgc gccagatcct
     5581 cgccaagcag gaaaccattg aaacgcaggc cgagcgcatc gatcgcttcc gcgatccggg
     5641 gcagcgtgaa gcgatgctcc tgcacgtgaa acaggaggtc ccggcattcg ccggtgacat
     5701 agaagtcgct acgacgcgtg aggcggtcga tttgcacggc atgatccgac gacagcagat
     5761 cctgacggca gcgccggata tcggtatcgg tcggtaagta ccccgccgcg gcgatgaatg
     5821 cccgcgccgc caccacgtcc tggcgcgatc gctcgctata gaggcccacg agcatgtgac
     5881 cgccgggtgc cagcatcgcg acgagctcgc gccatccggc caccgggtcg gccagatggt
     5941 gcaggacgcc caccgacgaa atgacgtcga acctccgccc gagcgccgcg acgttcatga
     6001 tgtcggcctg cgcgtgctcg aggttggcaa cgtaaagttc gcgcgccttg cgctcggcat
     6061 acgcgaggct cgcaaggctc aaatcgatgg cgagcacgcg cgcgcgggga aacgcatacg
     6121 cgagctcgat cgactcctgc cccgtgccgc agccggcgac gaggatgtcg agatcacgct
     6181 cgcccgattg ccgcagctcg aggggaaagc gcccgccggg acctgcaccg gccggcgacg
     6241 tcggcgccgg cgagggcagc cgcacccaac ttggatacgg atgctcctcg tattgccggc
     6301 gcacgtcgat cgagacccga tcgtcgattt cggtcagccg gcgaaggcga tcgcggaggc
     6361 cgcgctcctc caatggctcg cgcagttgct gcgtgagcag cgcgtccagc ggttccggcc
     6421 atgtccgggc gagcagcgct tcacgctgcg gcattgaaat cagcgggaag tacgcagcga
     6481 cgatggcgac gggcaccggg gcaaccgcct cgccgcgttc gagcgaggcg gcgacgacgt
     6541 tgcgcagcgc ggatgcgcgc tcgatttccg tgcctgtgca cgcgaagaca tagtcgtcga
     6601 tgaagcattg ccgcgcgagc gcacagcaga atgccagccg gtcgtcgttc gcggggtgac
     6661 ggctctcgat cgcggcgtcg agaagcgcga accttgcgag cgtgagaaag cgctccatcg
     6721 acaggtcgca caccggcgcg ctttcgagca acgcgcgaag cagcgcgtcg tcgaagacga
     6781 cgccaggatc cacgccgccg agcaagtcgc ccagcgtgac acgcgccggc cacgcgatcc
     6841 atgcgcgatc gatgcactcg cgcatcgccg cgccgctcgc gagcaggcga atgcaagcgg
     6901 ccgacagctc cgacggccgc gcccacggcg ccgcgagcgc gcgcagcgcc attcgccgca
     6961 cgtcgtcatc ggcgtggcga agctcggccg cgacgagcga ttgaacgaag cccgcgatcg
     7021 cgtcgggcgt ctcccgccgg atgagcgctt tggcatacgc ttgcgccgca tcgtcgaatg
     7081 cgccaaggtc gctgagtgcg ctcgcgaggt tgtaccggac gtcgggcacg tctcgcgttt
     7141 cgagcgcctt tcgatagatc tgcacggcct cggccgcgcg cccctgctcg cgcagcacat
     7201 tcgcgaggtt gttctgcgca tcggcgtagt cgggcttcaa cgataacgcg gtgcgaaagc
     7261 ttgcgatggc ctcgtcgagc cggccctggt cgtggagcgc aatgccgagt ccgttgtgcg
     7321 cgcgcggata ggcgtgcgcg agggcgagcg cctggcggaa gcgggcgatt gcctcgtcgt
     7381 tacggccgag cgcctgcagc acgatgccgg cgtcgttgta atacgtcggt tgaaccggat
     7441 tgaccatgat tgcgcgatcg atcagctcga gcgcggcctc gttgcgcccg acctggtgcg
     7501 cgatcatgcc gagtcgatgc agcgcatccg gctgatccgg cgcgttctgc agcacgctgc
     7561 gataacccgc ctcggcttcc gacaaccgtc cgtgcgcgtg atgcccggcc gcgtcggaca
     7621 atgcctttgc aagcgacgac ctcatgttgc ctcaacgata acgccgcgcg gcgccacgta
     7681 caacgacgaa aaaacggggg gccgaagccc cccgcgaatc aactgcggta ctgccgttct
     7741 tcgtcgctta tttgaaccga tagttgacct ggccgtagac gaagcgggcg cgcgggtcgt
     7801 agtacgacgg atcgaagccc acgatga
//
LOCUS       DASSVX010000313         8422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_563962, whole genome shotgun sequence.
ACCESSION   DASSVX010000313 DASSVX010000000
VERSION     DASSVX010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8422
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563962"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VFO53_13380"
     CDS             complement(<1..490)
                     /locus_tag="VFO53_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8877318.1"
                     /translation="MANFARAQLRRQRTSLALFLGPALVLLACFFIAPVIVDLVLAFT
                     DMDRTLAVTTLTLENFERMATRDSRLLASLGLTAVFVLATLGIFNVTFALLLALTTTA
                     ASKAAGAFFRAVWLLPRMSPSVVYALLWLWVVDPNERGLLNQVLVHVLGMAPADLRNN
                     HPL"
     gene            complement(541..1896)
                     /locus_tag="VFO53_13385"
     CDS             complement(541..1896)
                     /locus_tag="VFO53_13385"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8877319.1"
                     /translation="MNWKLAVAATLGAALALAALPAFADDVVIKVWSRADRSGPLRAG
                     NIVAAGETLNKLLAAAGSEKRVKIELNETNAKGYDEDALDLLKAFAVDKGPDIFVLAH
                     EWTGAFAAAGYAWNLEDHIKKNPEFYGDVIAPLWQAVTYKGARYGVPQDSEVRMLFLN
                     NDNLRKLGMSDADIAALPAKVDAGQFTANDLCDLAGQAVQKGVAKYGILHRPNAGPDF
                     QMLTESFGLVPYDKATAKLQASKAALRSFYTWVKYCTDKKALFANNTSMSWDAIEQVA
                     FTGDEAFAFFHGVWRVTDQMKGLNLKTRDEYFRRFTWINAPAGKKGGKPSNLSHPIAY
                     AVSTKSAHKDLAAYIVGLASQPYPNLQHAVTTNHTPINYGEQSMPEIVDKGWALVAAT
                     PMLKYASFMPNHPKIGQYNSIIFKGIQGVETGRLTAQAATDFVVDELENELGKDVEIR
                     N"
     gene            complement(1969..2655)
                     /locus_tag="VFO53_13390"
     CDS             complement(1969..2655)
                     /locus_tag="VFO53_13390"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET8877320.1"
                     /translation="MIAHRGSSARHADNTWAAFNAAVAEGADAIECDVMSTHDGTLVV
                     RHDLTLAGRQIADLDAAQIEAIAPGTVRLADVIAWAAGARIDLLVEVKHPDAAEAIGA
                     LVSASAWREHIVMAGFHGPSLAALKQADPGLRTSFMMGSVVTADDLVLLATRYRADGV
                     HPCWEGRAARPHRLLDAGFVDRVRSAELTLTLWHEEREEELCALVALAPDAICTNTPA
                     VLRAIVGARD"
     gene            complement(2667..3713)
                     /locus_tag="VFO53_13395"
     CDS             complement(2667..3713)
                     /locus_tag="VFO53_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET8877321.1"
                     /translation="MEPRLSDEGLRGAFVTAADVARRAGVSRSAVSRAFTPGASVSKD
                     ARQRIVEAADHLGYRINRLAQSLNNARSNLVGLVGTDLQQPFHADLLATLSGALLADG
                     FQCMLLNAANATHDMGALITRVLEYRVCAIVVMAGTPPARIVVECLHNGVPVILINKL
                     LPKVAVDTVISDHAAGGRAAAEQLLAAGCRRIGVVSSAARTTSLTTRIDAFVACATAA
                     RVRVRLWRKGPTDYRSGYEAALDLLKDGAVDGVFCVTDLLALGFLDAARSECRRRIPD
                     DLSVIGFDDIPQAAWSAYRLTTFRQPSADLAAAVMGAIRRRAAEPGAPPQLATVPVQL
                     VARSTVRSAHGRRS"
     gene            complement(3815..4249)
                     /locus_tag="VFO53_13400"
     CDS             complement(3815..4249)
                     /locus_tag="VFO53_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET8877322.1"
                     /translation="MTFDEFRPGQVLTYGPATLSEAQILAFAREYDPQWFHVDVERAR
                     TGRWQGLIASGWQTCGIAMRLVVEGALRGSESFGSPGLEYLKWLGPVRPGDLLTLVAE
                     VLEVRRSERQPTLGILRWRWRLLNQDDIAVLELVATNLFDLA"
     gene            complement(4246..4983)
                     /locus_tag="VFO53_13405"
     CDS             complement(4246..4983)
                     /locus_tag="VFO53_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HET8877323.1"
                     /translation="MTWSIVARDTHGALGVAVASRFFAVGALCPYARSGVGALATQAL
                     VNPGYGIAGLDRLVQGDSPRQVIEALVGDDDGREHRQVHLIDAAGRTAAYTGAQCIGW
                     CGDLAADGFSVAGNMLAGERVIGDTAEAFRHHAHLALAERLLLALEAGEAAGGDKRGK
                     QAAALRIVTTEDYPMLDLRVDDHVEPLAELRRLYDVSLERFQAFVTCLPTRARPAGIT
                     DRATIEREIERFRASRAATMHDATRGR"
     gene            5056..6624
                     /locus_tag="VFO53_13410"
     CDS             5056..6624
                     /locus_tag="VFO53_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HET8877324.1"
                     /translation="MSTPAAPPASFNLASHLMSRNAARADRIAYIDDRERLSYGELAK
                     RIRRFASVLRERGLRREERVLLLAHDGVEWPVAFLGALYAGIVPVAVNTLLTAADYAY
                     MLAHSRARAAIVSNALLPMLERALQSAPSDVETVWVIGAPETIADRRDFAADVASAPE
                     ARPAATHADDVAFWLYSSGSTGAPKGTVHTHANAYWTDTLYAQGVLRLTENDVCFSAA
                     KLFFAYGLGNALTFPLSAGATTVLMAERPTPMAVFRRLAEHRATVFYGVPTLYAAMLA
                     SPDLPVRAALALRLCVSAGEALPRDIGERFTAHLGVEILDGIGSTEMLHIFLSNRSGS
                     VRYGTTGVPVPGYEIELRGEDGRPAADGDVGDLYVRGPSAALMYWTSRDKSRGTFQGE
                     WTKSGDKYVRRDDGFYMYSGRADDMLKVSGLYVSPFEVEGALVQHPAVLEAAVIGKPD
                     ADGLTKTKAFVVLKPGHAGDAALEDTLKTFVKDKLAPYKYPRSIEFLAELPKTATGKI
                     QRFKLREREQAGAP"
     gene            6621..7487
                     /locus_tag="VFO53_13415"
     CDS             6621..7487
                     /locus_tag="VFO53_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8877325.1"
                     /translation="MTAVAFVDIEYDGRAITLEYAWVEPASAPVANDAPRREAADVPL
                     VVFLHEGLGSLSMWKDYPQRLCDACGCRGLVYSRAGYGRSTPRGPDERWPVDFMHREA
                     QGVLPRLFDRLGVGADALPPWLLGHSDGGSIALIYAASFPDAVAGVVAVAPHIFVEDV
                     SIASIVAARDAYGATDLRARLARYHDDPDSAFFGWNDIWLDPAFRDWSIEPVLDRLQC
                     PILAVQGEDDEYGTLAQVTGIRIRVPHSETVVLANCGHSPQRDQPEALTRAITGFIAR
                     HSGARANKGGVS"
     gene            7484..>8422
                     /locus_tag="VFO53_13420"
     CDS             7484..>8422
                     /locus_tag="VFO53_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877326.1"
                     /translation="MIFRRATLVRAVLAAALCAAASGAAAQGKIKVGFMLPYTGTYAA
                     LGTAIENGFRLYVQEQGGKLGGRELEYFKVDDESEPSKAVDNINKLIKRDNVDVVVGT
                     VHSGVVMAMAKVAKDTNTLLVIPNAGAEAVTGPMCAPNIFRSSFSNWQPGYAMGKVAG
                     ERGAKKVATITWKYAAGDESVEGFKEGLKASGGDVVKELTLPFPNVEFQALLTEIAAT
                     KPDAVYAFFAGGGAVKFVKDYAAAGLNKTIPLYGPGFLTDGTLEAQGAAAQGMLTTLH
                     YADGLDVPKDKAFRADYNKAYKASPDVYAVQGYDAAQ"
BASE COUNT         1393 a         2846 c         2825 g         1358 t
ORIGIN      
        1 gcagcggatg gttgttgcgc aggtctgccg gcgccatgcc gagcacgtgg acaagcacct
       61 ggttgaggag gcctcgctcg ttcgggtcga ccacccagag ccatagcagc gcatagacca
      121 ccgacgggct catgcgcggc agaagccaca cggcgcggaa gaacgcccct gccgccttcg
      181 acgcggccgt cgtcgtcagc gcaagcagaa gggcgaacgt gacgttgaag atcccgagcg
      241 ttgcgagcac gaacaccgct gtgagcccga gcgatgcgag gagccgggaa tcgcgcgtgg
      301 ccatgcgctc gaagttttcg agcgtgaggg tcgtcaccgc aagcgtgcgg tccatgtcgg
      361 tgaacgcgag gaccaggtcg acgattaccg gcgcgatgaa aaagcacgcg agcagcacga
      421 gcgccggccc caggaacagc gccaggctcg ttcgctgccg gcgaagctgg gcgcgcgcga
      481 aattggccat gacgagccca cgacccgagc cggcgcggtg cgcaccggct ccgagcggcc
      541 tcagttacgg atctcgacgt ccttgccgag ctcgttctcg agctcgtcga cgacgaagtc
      601 ggtggccgcc tgcgcggtca ggcggccggt ctcgacaccc tggattccct tgaagatgat
      661 cgagttgtat tgcccgatct tggggtgatt cggcatgaag ctcgcatact tcagcatcgg
      721 tgtcgcagcg acgagcgccc agcccttgtc gacgatctcg ggcatcgact gctcgccgta
      781 gttgatcggc gtgtggttgg tggtgaccgc gtgctgcagg ttcggatagg gctggcttgc
      841 gaggccgacg atgtacgcgg cgaggtcctt gtgcgccgac ttggtcgaca ccgcgtaggc
      901 gatcgggtgc gacaggttcg acggcttgcc gcccttcttg cccgccggtg cattgatcca
      961 cgtgaacctg cggaagtatt cgtcgcgggt cttcaggttg agccccttca tctggtcggt
     1021 gacgcgccag acgccatgga agaacgcaaa cgcttcgtcg ccggtgaacg ccacctgctc
     1081 gatcgcatcc catgacatcg acgtgttgtt cgcgaacagc gccttcttgt cggtgcagta
     1141 cttgacccac gtgtagaagc tgcgcagcgc cgccttcgac gcttgcagct tcgccgtcgc
     1201 cttgtcatac ggcaccaggc cgaagctctc ggtcagcatc tggaagtccg gtcccgcgtt
     1261 cggacgatgg agaatgccgt acttggcgac gcccttctgg accgcctgtc ccgcgaggtc
     1321 gcacaggtcg ttcgcggtga actggcccgc gtccaccttc gccggcagcg ccgcgatgtc
     1381 ggcgtcgctc atgccgagct tgcgcaggtt gtcgttgttg aggaagagca tgcgcacttc
     1441 ggaatcctgc ggcacgccat agcgcgcgcc cttgtacgtc acggcctgcc acagcggcgc
     1501 gatgacgtcg ccgtagaact cgggattctt cttgatgtgg tcctcgagat tccacgcgta
     1561 tccggctgct gcgaacgcgc ctgtccattc gtgcgcgagg acgaagatgt ccggaccctt
     1621 gtcgacggcg aacgccttca ggaggtcgag cgcgtcttcg tcgtagccct tggcattcgt
     1681 ttcgttgagc tcgatcttca cgcgcttttc gcttcccgcc gcagcgagca gcttgttcag
     1741 cgtctcgccg gccgccacga tgttgccggc gcgcagtgga cccgaccggt cggcgcgcga
     1801 ccagaccttg atgaccacat cgtccgcaaa agcgggcagc gccgcgagag cgagcgctgc
     1861 gccgagcgtt gcggcaactg caagcttcca gttcatgagg cctcctcgtt tgagcatgcg
     1921 agcgtgagcg gcggaatcaa catcagggcc gcgagcgtgg ggattcattc aatcgcgcgc
     1981 accgacgatc gcgcgcaaaa ccgccggggt attcgtgcat atcgcgtcgg gtgcaagcgc
     2041 cacgagcgca cacagctcct cttcgcgctc ctcgtgccaa agggtgagcg tcaactccgc
     2101 gctccgtacg cgatcgacga accccgcgtc gagcagccga tgcggtcgcg ccgcgcgtcc
     2161 ttcccagcac ggatgcacgc catcggcgcg atagcgagtc gcgagaagca cgagatcgtc
     2221 ggcagtcacg acgctaccca tcatgaacga cgtgcgcagg ccgggatccg cctgcttcag
     2281 cgccgcgagc gagggtccgt gaaagccggc catcacgatg tgctcgcgcc acgcgctcgc
     2341 cgaaacgagc gcgccgatcg cctcggccgc atccggatgc ttgacctcga ccagaagatc
     2401 gatccgcgcg cctgcggccc acgcaatgac gtcggcgagg cgcaccgtgc cgggcgcgat
     2461 cgcctcgatc tgcgccgcgt cgaggtcggc gatctgccgt ccggcgagcg tgaggtcgtg
     2521 gcggacgacg agcgttccgt cgtgcgtcga catgacgtcg cactcgatcg cgtcggcccc
     2581 ttcggcgacg gccgcgttga acgccgccca ggtgttgtcc gcgtgccgcg cgctgctgcc
     2641 gcgatgtgcg atcacgcgag gacgcgtcac gatcgccgcc cgtgcgcgct gcgcaccgtc
     2701 gaacgcgcga caagttgcac tgggaccgtc gcgagctgcg gcggcgcgcc cggttcggcc
     2761 gcccggcggc ggatcgcgcc catgactgcc gcggcgagat ccgcgcttgg ttgacgaaac
     2821 gtcgtcaacc gatacgcact ccacgctgcc tgcggaatgt cgtcgaagcc gatcaccgat
     2881 agatcgtcgg gaatccggcg ccggcactcg gaacgggcgg catcgaggaa gccgagggcg
     2941 agcaagtcgg tgacgcagaa cacgccatcg accgcgccgt ccttcagcag gtcgagcgcc
     3001 gcttcgtatc ccgagcgata gtcggtcggc ccttttcgcc acaggcgcac gcgtacgcgc
     3061 gcagcagtgg cgcaggcgac gaacgcgtcg atacgcgtcg tgaggctggt cgtgcgggcg
     3121 gcactcgaca ctacgccgat gcgccggcat cccgccgcca gaagctgctc ggcggcggct
     3181 cgtccccccg ccgcatgatc cgagatgacg gtatcgacgg cgaccttggg cagcagcttg
     3241 ttgatcagaa tcaccggtac gccgttatgc aggcattcga cgacgattcg cgccggaggc
     3301 gtgccggcca tcacgacgat cgcacaaacg cggtattcga gcacgcgcgt gatcaacgcg
     3361 cccatgtcgt gcgtcgcgtt ggctgcattg agcagcatgc actggaagcc gtcggccagc
     3421 agcgcgcccg aaagcgtcgc gagcagatcg gcgtgaaacg gctgctggag atccgtgccg
     3481 acgagaccca ccaggttcga ccgcgcgttg ttgaggctct gggccagccg attgatgcga
     3541 taacccagat gatcggcagc ttcgacaata cgctggcgcg catccttcga aacgctggcg
     3601 cccggcgtga acgcgcgcga caccgcggag cgcgagactc ccgcgcgcct cgcgacatcg
     3661 gccgccgtca cgaacgcgcc tcgtagaccc tcatcggaca accgcggctc catcggcatt
     3721 gcacacgtgt gcacggcatg ataggacgcg atgcacgcgt gtgcaagccc gcatgcgcgt
     3781 tcgcatgaat ccgagcagat cgaagtacgc gccgctacgc gaggtcgaaa agattcgtcg
     3841 ccaccaactc cagcaccgcg atgtcgtcct ggttgaggag tcgccagcgc cagcgaagaa
     3901 tgccgagcgt cggctggcgc tccgagcgcc gcacttcgag cacttcggcg accagcgtca
     3961 gcaggtcgcc cggacgaacc ggtccgagcc atttcaggta ttcgagcccc ggcgagccga
     4021 acgactcgga gccgcgcaac gcgccctcga cgacgaggcg cattgcgatg ccgcaggtct
     4081 gccagccgct ggcgatgagt ccctgccagc gtccggttct cgcgcgttcg acgtcgacat
     4141 ggaaccattg cggatcgtat tcgcgagcga acgcgaggat ctgcgcttcg gacagcgttg
     4201 ccggaccgta cgtcagaacc tgccccggac ggaactcgtc gaacgtcatc gcccgcgcgt
     4261 cgcgtcgtgc atcgtcgcgg cgcgggacgc gcggaagcgc tcgatttcgc gctcgatcgt
     4321 cgcgcgatcg gtgatgccgg ccggtcgcgc gcgtgtcggc aggcacgtga cgaacgcctg
     4381 gaagcgctcc aggctcacgt cgtaaagccg ccgcagttcg gcgagcggct cgacatggtc
     4441 gtcgacgcgc aggtcgagca tcggatagtc ctcggtcgtg acgatgcgca atgccgccgc
     4501 ctgtttgccg cgcttgtcgc cgcccgccgc ttcgcccgcc tccagcgcaa gcagcaaccg
     4561 ctcggcaagc gcaagatgcg catggtgccg gaatgcttcc gccgtgtcgc cgatcacgcg
     4621 ttcgccggcc agcatgttgc cggccaccga aaagccgtcg gccgcgagat caccgcacca
     4681 gccaatgcat tgcgcgccgg tgtaggccgc ggtgcggccc gcggcgtcga tgaggtggac
     4741 ctgccgatgc tcgcgaccgt cgtcgtcgcc gacgagcgcc tcgatcacct gtcgcggcga
     4801 atccccctgc accagccggt cgagcccggc aatgccatag ccgggattga ccagcgcctg
     4861 ggttgcgagc gcgcccacgc cgcttcgtgc gtacgggcac agcgcaccca cggcgaagaa
     4921 gcggctggcg acggcgacgc cgagcgcgcc gtgcgtgtcg cgtgccacga tcgaccatgt
     4981 catccggacc tccgcatcgc gtccgggcac tataatgcag cgcaccgaat cgcaaacgcc
     5041 gacatcaacc gcgtcatgtc gacccccgcc gccccgcccg cgtcgttcaa tctcgcctcg
     5101 cacctgatgt cgcgcaatgc ggcgcgcgcc gatcgcatcg cctatatcga cgatcgcgag
     5161 cggctgtcgt atggcgaact cgcgaagcgt atccgccgct tcgcatcggt gctgcgcgag
     5221 cgtggcctgc gccgcgaaga gcgcgtgctg ctgctcgcgc acgacggtgt cgaatggccc
     5281 gttgcgttcc tcggcgcgct ctacgccggc atcgtgccgg tcgcagtgaa tacgctgctg
     5341 accgcggccg actacgcgta catgctggcg cacagccgag cgcgtgccgc gatcgtgtcg
     5401 aacgcgctgc tgccgatgct cgagcgggcg ctgcaaagcg cacccagcga cgtcgagacc
     5461 gtgtgggtca tcggcgcgcc cgagacgatc gccgatcggc gcgatttcgc tgcggacgtt
     5521 gcgagcgcgc cggaagcgcg gcccgcggcg acgcacgccg acgacgtcgc gttctggctc
     5581 tattcgtcgg gctcgaccgg tgcgccgaag ggcaccgtgc acacgcacgc gaatgcgtac
     5641 tggaccgata cgctctacgc acagggcgtg ctgcgtctca ccgagaacga cgtctgcttc
     5701 tccgcggcca agctcttctt cgcctatgga ctcggcaacg cgctgacctt tccattgtcg
     5761 gcgggcgcga ccaccgtgct gatggccgag cggccgaccc cgatggcggt tttccggcgt
     5821 cttgccgagc atcgcgccac ggttttctac ggcgtgccga cgctttacgc cgccatgctc
     5881 gcctcgcccg acctgcccgt gcgcgcagct ctggcgctgc gtctttgcgt gtcggcgggc
     5941 gaggcgctgc cgcgcgacat cggcgagcgc ttcacggctc acctcggggt cgagatcctc
     6001 gacggcatcg gatcgaccga aatgctgcac atcttccttt cgaatcgctc gggcagcgtg
     6061 cggtacggaa ccaccggcgt gccggtgccg ggctacgaga tcgagctgcg cggtgaggac
     6121 ggccggccgg ccgccgacgg cgacgtcggc gatctctacg tgcgcgggcc gagcgctgcg
     6181 ctcatgtact ggaccagccg cgacaagtcg cgcgggacgt tccagggcga gtggacgaag
     6241 agcggcgaca agtacgtgcg ccgcgacgac ggcttttaca tgtactcggg acgcgccgac
     6301 gacatgctga aggtgagcgg gctctacgtg tcgccgttcg aggtcgaggg cgcgctcgtg
     6361 cagcatcccg cggtgctcga agcggccgtc atcggcaaac ccgacgccga cggcctgacc
     6421 aagaccaagg cgttcgtcgt gctgaagccg ggccacgcgg gcgacgccgc gctcgaggac
     6481 acgctgaaaa cgttcgtcaa ggacaagctc gccccgtaca agtacccgcg gtccatcgag
     6541 ttcctcgcgg agttgccgaa gacggcgacc ggcaagatcc agcggttcaa gctccgcgag
     6601 cgcgagcagg ccggggcgcc gtgacagccg tcgcgttcgt cgacatcgaa tacgacggac
     6661 gcgcgataac cctcgaatac gcatgggtcg agccagcatc tgcgccggtc gccaacgatg
     6721 cgccgcgccg ggaagccgcc gatgtgccgc tcgtcgtgtt cctgcacgag gggctcggct
     6781 cgctttcgat gtggaaggac tatccgcaga gactgtgcga tgcgtgcggc tgccggggcc
     6841 tcgtgtattc gcgcgccggg tacggccgct cgacgccgcg cggtcccgat gagcgctggc
     6901 ccgtcgactt catgcaccgc gaggcgcagg gcgtgctgcc gcgcctgttc gatcggctcg
     6961 gcgtcggcgc cgatgcgctg ccgccctggc tgctcgggca cagcgatggc ggctcgatcg
     7021 cgctcattta cgcggcgagt ttcccggacg ccgtcgccgg cgtggttgcc gtggcgccgc
     7081 acattttcgt cgaggacgtg tcgatcgcga gcatcgtcgc tgcacgcgac gcgtatggcg
     7141 ccacggactt gcgcgcgcgg cttgcccgct accacgacga ccccgactcc gcgttcttcg
     7201 gctggaacga catctggctc gatccggcct ttcgcgactg gtcaatcgag ccggtgctcg
     7261 accggctgca atgccccata ctcgcagtgc agggcgaaga cgacgagtac ggtaccctcg
     7321 cgcaggtcac cggcatccgc attcgcgttc cgcacagcga aaccgtcgta ctcgcgaatt
     7381 gcggtcattc gccgcaacgc gaccagcccg aggcgctgac gcgcgcaatc accggtttca
     7441 ttgcgcgcca ttccggggcg cgcgcaaaca agggaggagt gtcatgattt ttcgtcgtgc
     7501 aacgctcgtg cgcgccgtgc tcgcggccgc actctgcgcc gccgcgagcg gcgccgctgc
     7561 gcaaggcaag atcaaggtcg gtttcatgct tccgtacacc ggcacctacg cggcgctcgg
     7621 caccgccatc gaaaacggct tccggttgta cgtgcaggag cagggcggca agctcggcgg
     7681 acgcgagctc gaatacttca aggtcgacga cgagtccgag ccgtcgaagg ccgtcgacaa
     7741 catcaacaag ctgatcaagc gcgacaacgt cgacgtcgtc gtcggcaccg tgcactcggg
     7801 cgtcgtcatg gccatggcca aggtcgcgaa ggacacgaac acgctgctcg tcattccgaa
     7861 tgcgggcgcc gaggcggtga cgggtccgat gtgcgcgccg aacatcttca gaagctcgtt
     7921 ctcgaactgg cagccgggtt acgcgatggg caaggtggcc ggcgagcgcg gcgcgaagaa
     7981 ggtggcgacg atcacgtgga aatacgccgc aggcgacgaa tcggtcgaag gcttcaagga
     8041 gggcctgaag gcatcgggcg gcgacgtggt caaggagctg acgctgccgt ttccgaacgt
     8101 cgagttccag gcactgctca ccgagatcgc ggcgaccaaa cccgacgcgg tctacgcgtt
     8161 cttcgcgggc ggcggcgcgg tgaaattcgt caaggactac gcggccgcgg gcttgaacaa
     8221 gacgatcccg ctctacgggc cgggctttct gaccgacggc acgctggagg cgcagggcgc
     8281 ggcggcgcag gggatgctga ccacgctgca ctacgccgac gggctcgacg tgccgaagga
     8341 caaggcgttt cgcgccgact acaacaaggc gtacaaggcg agtcccgacg tgtacgcggt
     8401 gcagggctac gatgcggcgc ag
//
LOCUS       DASSVX010000314         2041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1180352, whole genome shotgun sequence.
ACCESSION   DASSVX010000314 DASSVX010000000
VERSION     DASSVX010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180352"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VFO53_13425"
     CDS             <1..1147
                     /locus_tag="VFO53_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877327.1"
                     /translation="FTNSGELANRLMHPRYEVEREYAVRVMGRLTDEQVQALTSGIEL
                     DDGPARCEKVSDGGGDEDGSNHWYHVVLKEGRNREVRRLFEALGLMVSRLIRIRFGAL
                     AMPTHLKRGHLAELEAADVDAIVASAGLRAQQGRPPSRPKARNAHADGTPSSPGGRPK
                     SRPHKSRPHGQRGQAPFVPGQGRAGASGELPGDDQAGVSSQGSKSPRPANASPKKSGA
                     WKKRGANGKPANAGPQRPPQGDARRKPGKPHHHPPGQRPPRTGHNFDEVQPQSNANAS
                     PFGRTTLSAPGGAAAGYGNNKGSPRHRSGPRAQRAGNPNGHARSQAGAAAFNDRRDME
                     PVYKVPARPVTQTQIRSKRTRTVVQADIAADADGTPALRRLLNDGKS"
     gene            complement(1191..>2041)
                     /locus_tag="VFO53_13430"
     CDS             complement(1191..>2041)
                     /locus_tag="VFO53_13430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8877328.1"
                     /translation="PKLVAAVPFTPVPGARLLAPAATTRRALLACAQRELARGYSSLH
                     VLFVDETQSREGAAAGMLVRDGVQFHWRNAGYRDFADFLAAFNHDKRKKIRQERRRLA
                     EAGVRFVRKIGDEISAADRAFFFDCYKRTYGAHHSTPYLSLDFFDRIGATLAPHLLLV
                     LGYRNGRRLCAALDVFDDATLWGRYWGTREYVPGLHFEACYYQAIEFCIERGIASFEG
                     GAQGVHKLARGLLPVATHSLHAIADRSFAAAIADFCARERSDVAHSIGELESGSPFRS
                     DRSEET"
BASE COUNT          370 a          688 c          720 g          263 t
ORIGIN      
        1 gttcacgaac tcgggcgagc tcgcgaaccg gctcatgcat ccgcgctatg aagtcgagcg
       61 cgaatacgcg gtgcgcgtca tggggcggct caccgacgag caggtgcagg cgttgacgag
      121 cggcatcgag ctcgacgacg gtccggcgcg ctgcgaaaag gtgagcgacg gcggcggcga
      181 cgaagacggc tcgaaccatt ggtaccacgt cgtgctgaag gaagggcgca accgcgaagt
      241 acgccggctc ttcgaagcgc tcggactcat ggtgagccgg ctgattcgca tccgctttgg
      301 cgcacttgcg atgccgacgc acctgaagcg cggtcacctg gccgaactcg aagccgcgga
      361 tgtggacgcg atcgtcgctt ccgccggctt gcgcgcgcaa cagggccgtc cgccatcgcg
      421 gccgaaggcg cgtaacgcgc atgccgacgg cacgccatcg tcgccgggtg gccggccgaa
      481 gtcacggccg cacaagtcgc gaccgcatgg ccagcgcggt caggcgccgt tcgtaccggg
      541 ccaggggcgc gcaggcgcaa gcggcgaatt gcccggggac gaccaggccg gcgtgtcttc
      601 gcagggaagc aagtcgccac gcccggccaa tgcatcgccg aagaagtccg gcgcatggaa
      661 gaagcgcgga gccaacggga aaccggcgaa cgccgggccg cagcgtcctc cgcagggtga
      721 cgcgcggcgc aagccgggca agccgcacca tcatccgcca gggcagcggc cgccgcgcac
      781 cggacacaac ttcgacgaag tgcagccgca aagcaacgcc aacgcgtcgc cgttcgggcg
      841 cacgacgctg tccgccccgg gaggcgccgc ggccggctac ggcaacaaca agggatcgcc
      901 ccggcatcgc agcggcccgc gggcgcagcg ggcgggtaac ccgaatggcc atgcgcgcag
      961 ccaagcgggt gccgcggcct tcaacgatcg ccgcgacatg gaaccggtct acaaggtgcc
     1021 tgcgcgaccc gtcacgcaga cacagatccg ctccaaacgc acgcgtacgg tggtgcaggc
     1081 cgacattgcg gccgacgccg acggaacgcc ggcgttgcgc cgcctgctga acgacggaaa
     1141 gtcctgacgc cggaacgccg ggctggcgcg cgcgtgcgcg ggcgtcgcgc tcatgtttct
     1201 tcgctgcgat cgctgcggaa gggactgccc gattcgagct cgccgatcga gtgcgcaacg
     1261 tcgctgcgct cgcgcgcgca aaaatcggcg atcgccgcag cgaagcttcg gtcggcgatc
     1321 gcgtgcaacg aatgcgtggc gacgggcagg agcccgcgtg caagcttgtg cacgccctgt
     1381 gcgccgcctt cgaagctcgc gatgccgcgc tcgatgcaaa actcgattgc ctggtagtag
     1441 caggcctcga aatgcagtcc cggcacgtac tcgcgcgtgc cccagtagcg tccccacagc
     1501 gtcgcgtcgt cgaacacgtc gagcgccgcg catagcctgc ggccgttgcg gtagccgagg
     1561 acgagcagca gatggggtgc gagcgtcgct ccgatacggt cgaaaaaatc gagcgacagg
     1621 tacggcgtcg aatgatgcgc gccgtacgtg cgcttgtagc agtcgaagaa gaacgcacga
     1681 tcggcggcac tgatctcgtc gccgatcttg cgcacgaacc tgacacccgc ctcggcgagg
     1741 cggcggcgct cctggcggat cttcttgcgc ttgtcgtgat tgaatgccgc gaggaaatcg
     1801 gcgaaatccc gatagccggc gttgcgccag tggaactgca cgccatcgcg cacgagcatg
     1861 ccggccgcag caccttcgcg cgactgcgtc tcgtcgacga acagcacgtg cagcgacgaa
     1921 tagccgcgcg caagctcacg ctgcgcgcac gcgagcagtg cgcggcgggt cgtcgcggct
     1981 ggcgcgagca ggcgcgctcc gggtaccggg gtaaacggaa ccgcggcgac gagcttcggg
     2041 t
//
LOCUS       DASSVX010000315         6710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1547088, whole genome shotgun sequence.
ACCESSION   DASSVX010000315 DASSVX010000000
VERSION     DASSVX010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547088"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFO53_13435"
     CDS             <1..361
                     /locus_tag="VFO53_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET8877329.1"
                     /translation="DVVKLDILINGERVDALSVMVHRSVATYRGREVAAKMRSLIPRQ
                     MFDVAVQAAIGAQIIARENIKALRKNVLAKCYGGDITRKKKLLEKQKAGKKRMKSVGS
                     VEIPQEAFLAILQVGGK"
     gene            607..1431
                     /gene="lepB"
                     /locus_tag="VFO53_13440"
     CDS             607..1431
                     /gene="lepB"
                     /locus_tag="VFO53_13440"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET8877330.1"
                     /translation="MNFALILFVLTAVTGFVWLVDRLFFAARRAPGAPEPVWVEYSKS
                     FFPVLLVVFVLRSFLAEPFKIPSSSMRPTLEVGDFILVNKFIYGIRLPVIEQKIIPVS
                     DPSRGDVVVFRYPLNPSQDFIKRVVGLPGDVVEYRDKKLTVNGTPWPQRADGSYSYLE
                     GLRFETLEKSIEDTDGKPALTHTIGLNAQAQPVYPSNVRPFPGRENCDYNERGFKCRV
                     PAGHYFMMGDNRDNSDDSRYWGFVPDDHLRGRAFFIWFNWDDIASFAFKRVGSAIH"
     gene            1457..1813
                     /locus_tag="VFO53_13445"
     CDS             1457..1813
                     /locus_tag="VFO53_13445"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HET8877331.1"
                     /translation="MSGNERGLSMIGFLFTTIVIIIFALVAFRVGPSYIEYFTVQKAL
                     DQTMLDVQTPTAAEIRRSMDRRLSADYVDSVNAAEVAVGREGNAIVATLSWQKVLHMI
                     GNASILLEFEARSSRG"
     gene            1767..2519
                     /gene="rnc"
                     /locus_tag="VFO53_13450"
     CDS             1767..2519
                     /gene="rnc"
                     /locus_tag="VFO53_13450"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET8877332.1"
                     /translation="MRASSSSSKRARPAASEPSSPDPPDAGAPRVGVEHAFANPDLLR
                     QALTHRSYGTPNNERLEFIGDAVLGFVVALSLYERFPDAPEGHLTRARAHLVRENTLA
                     QVARRIGLGRAIRVGEGELKSGGTDRASILADAMEAVFGAIFLDAGFAPARAAIVAAY
                     GDVLRDADPARLGKDPKTRLQEALHKRRLPEPTYEIVGTTGKAHAQQFAVECRIPDLA
                     IVTRGSGVSRRAAEQDAATQALDLLPVERRHG"
     gene            2512..3405
                     /gene="era"
                     /locus_tag="VFO53_13455"
     CDS             2512..3405
                     /gene="era"
                     /locus_tag="VFO53_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET8877333.1"
                     /translation="MAEADTRHRCGHVAIVGRPSVGKSTLLNALVGARISITSRKPQT
                     TRHRISGILTEPGAQFIFVDTPGWQTQHRSRLNERLNRAVRDALAEVDVIVFAADAAR
                     FGAQDREVLDLLPRDVPVVAALTKIDALTDKRALLPRIAEIAALREFVAIVPISAEKG
                     TDLIALKHAVAEALPPGPALYEGDDITDRDERFLAAEFIREKIFRQLGDEVPYGTAVS
                     IESFEHDGGLRRIRATIFVAKTSQRAILVGEGGARMKTIASQARADMEGLFGGKVFLD
                     VWVRVKRRWSDSDAMLTRLGY"
     gene            3417..4190
                     /gene="recO"
                     /locus_tag="VFO53_13460"
     CDS             3417..4190
                     /gene="recO"
                     /locus_tag="VFO53_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET8877334.1"
                     /translation="MKQLRERHDDEPAFVLHTYAYRETSLIVEAFTERHGRVAMVARG
                     AKRPRSEMRGVLQAFQPLALSWAGAGELKTLVKAEWQGGLPLPGGAALLCGFYLNELL
                     LKLLAREDAHPALWRQYESALRALTANQAPGAQAATLRRFELRLLAELGYALTITRDV
                     ARDAPVEAGERYHYAFDRGPQHVAAEPRASWPVVRGETLLALATERYPDADIAAEAKR
                     LMRGILDHYLEKRHIFSRRMVRDLAALEDRDVTSEGNRS"
     gene            4187..4948
                     /locus_tag="VFO53_13465"
     CDS             4187..4948
                     /locus_tag="VFO53_13465"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HET8877335.1"
                     /translation="MIELGVNIDHVATLRQARRTYEPDPVWAAVEAHLGGADGITVHL
                     REDRRHIQDQDVRRLRDLVHIKLNLEMAATDEMVAIACALRPEMAMLVPEGREEVTTE
                     GGLDVAAQEGKLEAAIGKLTDAGIVTSVFIDADLPQVEAAARAGARVCEVHTGPYAHA
                     FHAKGRDAESRAVVEELDRIHIAGRAIRDLGMRFNAGHALNYINVQPVAALPGVRELH
                     IGHAIVSRSVFVGLREAVREMKALMRQAAKREPVA"
     gene            4966..5925
                     /gene="nagZ"
                     /locus_tag="VFO53_13470"
     CDS             4966..5925
                     /gene="nagZ"
                     /locus_tag="VFO53_13470"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET8877336.1"
                     /translation="MLGIQALELGAGDRARLADPRVGGVILFARNFASSAQLRALTHS
                     IHALRPELLIGVDHEGGRVQRFRNDEFTPLPPMRALGRQWDDEPAAATAAAFDVGATI
                     ARELRAHSVDFSFTPVLDLDHGVSAVIGNRAFHDDPVIVADLAGALVRGLAAGGVAAV
                     GKHFPGHGFVAADSHVDTPVDARALDDILRTDVVPFAALIQQGLAAIMPAHVVYPAVD
                     ARPAGFSAAWIGAILRGRLGFDGLVFSDDLEMTGARSAGDIVARADAALGAGCDVVLV
                     CNDFGVMDDLLARWTPPVQPALARRWERMARQFPSAVAPAGHS"
     gene            complement(5886..>6710)
                     /locus_tag="VFO53_13475"
     CDS             complement(5886..>6710)
                     /locus_tag="VFO53_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET8877337.1"
                     /translation="AGVPTVWLGLLNYMKQNDLRLSTLRMTTIGGSACPPALMQSLQD
                     DYGIRVLHAWGMTEMSPMGTTACLKSKHLDLAAGDRFAVGLKQGRPLYGVDMKITDEQ
                     GNALPHDGKSAGNLLVKGPWIASRYYRDDAVPTVDGWFPTGDVATIDEDGYLRLTDRA
                     KDVIKSGGEWISSIELENIAIGHPGVMEAAVIGVAHAKWDERPLLVVVKKPGAEVTRQ
                     ALLDFYVGRTAKWWMPDDVVFVDALPHTATGKLSKRALREQYRDYEWPAGATALGN"
BASE COUNT         1064 a         2308 c         2232 g         1106 t
ORIGIN      
        1 cgacgtcgtc aagctcgaca tcctgatcaa cggcgagcgc gtcgacgcgc tgtcggtgat
       61 ggtgcaccgg tcggtcgcga cctaccgcgg ccgcgaggtt gccgcgaaga tgcgctcgct
      121 gattccgcgg cagatgttcg acgtcgccgt gcaggcggcg atcggcgccc agatcatcgc
      181 gcgcgagaac atcaaggcgc tgcgcaagaa cgtgctggcc aagtgctacg gcggcgacat
      241 cacgcgcaag aagaagctgc tcgagaagca gaaggcgggc aagaagcgga tgaagtcggt
      301 cggcagcgtc gaaatcccgc aggaggcgtt cctcgcgatc ctgcaggtcg gcggcaagtg
      361 aatcggggtc agagctgtaa ttattgcggc gtggacggta cgggatgccc ggcaatctcc
      421 gccgcaataa ttacagctct gaccccgatt caacccatgt tgcggcgtgg acggtacggg
      481 atgcccggca atctccgccg caataattac agctctgacc ccgattcaac ccatgcgaca
      541 atgccgcgcc ggtgatcgcc gtacctcccg cagtccgttc tccccttcgc ccttttccga
      601 gattcgatga acttcgccct gatcctgttc gtgctgaccg ccgtgacggg cttcgtctgg
      661 ctggtcgacc gcctgttctt tgccgcgcgg cgggcgcccg gcgcccccga gcccgtgtgg
      721 gtcgagtatt cgaagtcgtt ctttccggtg ctgctcgtcg tcttcgtgct gcgctcgttc
      781 ctcgccgagc cgttcaagat tccgtcgtcg tcgatgcgcc cgacgctcga ggtgggcgac
      841 ttcatcctcg tcaacaaatt catctacggc atccggctgc cggtcatcga gcagaagatc
      901 atcccggttt ccgatcccag ccgcggcgat gtcgtcgtct tccgctatcc gctgaatccg
      961 tcgcaggatt tcatcaagcg cgtggtcggc ctccccggcg acgtcgtcga gtaccgcgac
     1021 aagaagctga cggtcaacgg cacgccgtgg ccgcagcgcg ccgatggctc gtacagctac
     1081 ctcgaagggc tgcgcttcga gaccctcgag aaaagcatcg aggacacgga cggcaagccg
     1141 gcgctgacgc atacgatcgg cctcaacgcg caggcccagc cggtgtatcc gtcgaacgtc
     1201 cgcccgttcc ccgggcgcga aaattgcgac tacaatgagc gcggcttcaa atgtcgggtt
     1261 cccgccgggc attacttcat gatgggcgac aaccgcgaca acagcgacga tagccgctat
     1321 tggggattcg ttccggacga ccacctgcga ggcagggcgt tcttcatctg gttcaactgg
     1381 gacgacatcg catcgtttgc attcaagcgc gtcggcagcg ccattcactg accggcggcg
     1441 cggcggaggg agcatcgtgt ccggaaacga gcgtggcctg tcgatgatcg gcttcctgtt
     1501 cacgacgatc gtgatcatca ttttcgcgct cgtcgcgttc cgcgtgggtc cctcctacat
     1561 cgaatacttc accgtccaga aggcgctcga ccagacgatg ctcgacgtgc agacgccgac
     1621 ggccgccgag atccgccgct cgatggatcg gcggctctcc gccgattacg tcgattcggt
     1681 caacgcggcc gaagtggcgg tcggacgcga aggcaacgcc atcgtcgcca cgctgtcatg
     1741 gcagaaggtc ctgcacatga tcggcaatgc gagcatcctc ctcgagttcg aagcgcgctc
     1801 gtcccgcggc tagcgaacct tcctcgcccg atccgccgga cgccggcgcg ccacgcgtcg
     1861 gcgtcgagca cgcgttcgcg aaccccgacc ttttgcgtca ggcgctgacg caccgcagct
     1921 atggcacgcc caacaacgag cggctggagt tcatcggcga cgcggtgctg ggcttcgtcg
     1981 tggcactcag cctctacgag cgctttcccg atgcgcccga agggcacctg acgcgggcgc
     2041 gcgcccatct ggtgcgcgaa aacacgctcg cgcaggtcgc gcggcgcatc ggcctcggtc
     2101 gcgcaatccg cgtcggcgaa ggcgaattga agagcggagg caccgaccgg gcctcgatcc
     2161 tcgccgatgc gatggaagcc gtgttcggcg ccatctttct cgatgccggc ttcgcgccag
     2221 cccgggcggc gatcgtcgcc gcctacggcg acgtgctgcg cgatgccgat cccgcgcgcc
     2281 tcggcaagga cccgaagacg cggctgcagg aggcgttgca caagcgccgg ctgcccgagc
     2341 ccacgtacga gatcgtgggc acgacgggca aggcgcacgc gcagcaattt gcggtcgaat
     2401 gccgcattcc cgatcttgcg atcgtgaccc gcggttcggg ggtgagccgg cgcgcggccg
     2461 agcaggatgc ggcgacgcag gcgctcgact tgctgccggt cgagcggcgc catggctgaa
     2521 gccgacaccc gccatcgctg cggtcacgtc gccatcgtcg gccgcccctc cgtcggcaaa
     2581 tcgacgctgc tgaacgcgct ggtcggcgcg cgcatcagca tcacgtcgcg caagccccag
     2641 acgacgcgcc accgcatcag cggcatcctc accgagcccg gcgcgcagtt cattttcgtg
     2701 gacacgcccg gctggcagac gcagcaccgg tcgcggctga atgagcggct caatcgcgcg
     2761 gtgcgcgacg cgcttgccga ggtggacgtc atcgtgttcg ccgccgacgc cgcgcgcttc
     2821 ggggcgcagg atcgcgaagt gctcgatctg ctgccgcgcg acgtgccggt cgtggcggcg
     2881 ctcaccaaga tcgatgcgct caccgacaag cgcgcgcttt tgccgcggat tgccgagatc
     2941 gctgcgctgc gcgagttcgt tgcgatcgtg ccgatttccg ccgaaaaagg caccgatctc
     3001 atcgcgctga agcacgcggt tgccgaagcg cttcctcccg gtcccgcgct gtacgaaggc
     3061 gacgacatca ccgaccgcga cgagcgcttt ctcgccgccg aattcatccg cgaaaagatc
     3121 ttccgccagc tcggcgacga ggtgccgtac ggcacggcgg tgtcgatcga aagcttcgag
     3181 cacgacggcg ggctgcgacg cattcgcgcg acgatcttcg tcgccaaaac gagccagcgt
     3241 gccatcctgg tcggcgaggg cggcgccagg atgaagacga ttgcctcgca ggcgcgcgcc
     3301 gacatggagg ggcttttcgg cggcaaggtg ttcctcgacg tgtgggtgcg cgtcaagcgc
     3361 cggtggtccg acagcgacgc gatgctgacg cgcctcggtt actgaagagc gcgcgcatga
     3421 agcaactgcg cgagcgccac gacgacgagc cggcgttcgt gctgcacacg tacgcatacc
     3481 gcgagacgag cctcatcgtc gaggcgttca ccgagcgcca cggccgggtg gcgatggtcg
     3541 cgcgcggcgc caagcgtccg cgctccgaga tgcgcggcgt gctgcaggcg ttccagccgc
     3601 tcgcgctgtc gtgggccggc gcgggcgagc tcaagacgct ggtcaaggcc gagtggcagg
     3661 gtggcctgcc gcttcccggg ggtgcggcgc tgctctgcgg tttctatctc aacgaattgc
     3721 tgctgaagct gctcgcccgc gaggatgcgc atccggcgct gtggcgccaa tacgaatcgg
     3781 cgctgcgcgc gctgaccgcc aaccaggcgc ctggcgcgca ggcggcgacg ctgcgccgct
     3841 tcgaactgcg tctgctcgcc gagctcggct acgcactcac gatcacgcgc gacgtcgcgc
     3901 gtgacgcgcc cgtcgaagcg ggcgaacgtt accattacgc attcgatcgc ggcccgcagc
     3961 acgtcgccgc cgagccacgc gcgtcgtggc cggtggttcg tggcgaaacg ctgctcgcgc
     4021 ttgcgaccga acgctacccc gacgccgaca tcgcggccga agcgaagcgg ttgatgcgcg
     4081 gcatcctcga ccactacctc gagaagcggc acatcttcag ccgccgcatg gtgcgcgatc
     4141 ttgcggcact ggaggacagg gacgtgacca gcgaaggaaa ccgatcgtga tcgagcttgg
     4201 cgtgaacatc gaccatgtcg cgacgctgcg gcaggcgcgt cgcacttacg aacccgatcc
     4261 cgtgtgggcc gcggtcgagg cgcacctcgg cggtgccgac ggcatcaccg tccatctgcg
     4321 cgaggaccgg cggcacatcc aggaccagga cgtgcgacgc ctgcgcgacc tcgtgcatat
     4381 caagctcaac ctcgagatgg cggctaccga cgaaatggtc gccatcgcct gcgcgctgcg
     4441 tcccgaaatg gcgatgctcg tgcccgaggg ccgcgaggaa gtgacgaccg aaggcgggct
     4501 cgacgtcgcc gcccaggagg gcaagctcga ggccgcaatc gggaagctga cggatgcggg
     4561 catcgtcacc tcggtgttca tcgacgccga cctgccgcag gtcgaggcgg ccgcccgcgc
     4621 cggcgcccgc gtctgcgagg tgcataccgg tccctacgcg cacgcgttcc atgcgaaagg
     4681 gcgcgacgcc gaaagccgcg ccgtggtcga ggagctcgac cgcattcaca tcgccggtcg
     4741 cgcgatacgc gatctcggca tgcgtttcaa cgcgggccac gcgctcaact acatcaacgt
     4801 gcagccggtc gcagcgcttc ccggcgtgcg cgagctgcac atcggccatg cgatcgtgtc
     4861 gcgcagcgtg ttcgtcggct tgcgcgaggc ggtgcgcgag atgaaggcgc tgatgcggca
     4921 ggcggcgaag cgggagccgg tcgcgtgaat gcgcgggggc ccgtgatgct cggcatccag
     4981 gcgttggagc ttggcgcggg cgaccgcgcg cgcctcgccg atccgcgcgt cggcggcgtg
     5041 attctgttcg cgcgcaactt cgcgtcgtct gcgcaattgc gcgcgctgac ccactcgatt
     5101 cacgcgctgc gaccggaact gctcatcggc gtcgatcacg aaggcgggcg cgtgcagcgc
     5161 tttcgcaacg atgaattcac gccgctgccg ccgatgcgag ccctggggcg gcaatgggat
     5221 gacgagcctg cggcagcgac cgcggccgcc ttcgatgtcg gcgcgacgat tgcgcgcgag
     5281 ctgcgcgcac acagcgtcga tttcagcttc acgccggtgc tcgatctcga tcacggggtc
     5341 agtgcggtga tcggcaatcg cgcctttcac gacgatccgg tgatcgtcgc cgacctcgcc
     5401 ggcgcgctgg tgcgcggact cgccgcgggc ggcgttgccg ccgtcggcaa gcatttcccc
     5461 ggacacggct tcgtcgcggc cgactcgcac gtcgacacgc ccgtcgacgc gcgcgcgctc
     5521 gacgatatcc tgcgcaccga cgtcgtgccg ttcgccgcgt tgatccagca ggggctcgcc
     5581 gcgatcatgc ccgcgcacgt cgtctatccg gcggtggatg cgcgcccggc gggcttttct
     5641 gccgcctgga tcggcgccat cctgcgcggc cggctcggtt tcgacggcct cgtgttctcc
     5701 gacgatctcg aaatgacggg cgcccgttcg gcgggcgaca tcgtcgcgcg cgccgacgcg
     5761 gcgctcggcg cgggatgcga cgtcgtcctc gtatgcaacg acttcggcgt catggacgac
     5821 ctgctggcgc gctggacgcc gcccgtgcag ccggcactcg cccggcgatg ggagcggatg
     5881 gcgcgtcagt tcccgagcgc cgtggcgccc gcgggccatt cgtaatcgcg atactgctcg
     5941 cgcaacgcgc gcttcgacag ctttcccgtc gcggtgtgcg gaagcgcgtc gacgaacacc
     6001 acatcgtcgg gcatccacca cttcgcggtg cggccgacgt agaagtcgag cagcgcctgg
     6061 cgcgtcacct ctgcaccggg tttcttcacg acgacgagca gcggccgctc atcccacttc
     6121 gcgtgggcca cgccgatgac cgcggcctcc atcacgcccg gatgcccgat cgcgatgttc
     6181 tcgagctcga tcgagctgat ccattcgccg cccgacttga tcacgtcctt cgcgcgatcg
     6241 gtgagccgga ggtagccgtc ctcgtcgatc gtcgcgacgt cgccggtcgg gaaccatccg
     6301 tcgaccgtcg gcacggcatc gtcgcggtaa tagcggcttg cgatccacgg gcccttcacg
     6361 agcaggttgc ccgcgctctt gccgtcgtgc ggcagcgcgt tgccctgctc gtcggtaatc
     6421 ttcatgtcga cgccgtagag cggacgaccc tgcttcagcc cgacggcgaa ccggtccccc
     6481 gccgcaaggt cgagatgctt cgacttcaag caggcggtgg tgcccatcgg gctcatctcg
     6541 gtcatgcccc acgcatgcag cacgcgaatc ccatagtcat cctgcagcga ctgcatcaac
     6601 gcaggcggac aggcggagcc gccgatcgtc gtcatcctga gcgtcgacag gcgcaggtcg
     6661 ttctgcttca tgtaattcag caggccgagc cacacggtcg gcacgccggc
//
LOCUS       DASSVX010000316         6934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_347542, whole genome shotgun sequence.
ACCESSION   DASSVX010000316 DASSVX010000000
VERSION     DASSVX010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6934
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347542"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1803
                     /locus_tag="VFO53_13480"
     CDS             112..1803
                     /locus_tag="VFO53_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HET8877338.1"
                     /translation="MRLPAFYESFLYLVLHWIVLATSWNILSGYSGYFSFGHGAFYGA
                     GVYTTAVLAGTFGVPFLWTLPAAGIVAALLGVGLGAVVFRVRRVRGELFALLTLAVTF
                     VLATIVQNTPIDGGPGVYLSAVAVPAVAPTASGAFYVMALALAVVALVLARAIARSRL
                     GLGLFAIHDDEDAAEVMGVPTYRYKLAAFGISCALAGIAGGIQALFVSYVTASSTFNI
                     TVPLTVVLMCVLGGTRHWAGPALGATAITLLLYASTAADQAVAGKAITGAILVAAVLF
                     MPDGVLGLVRRRKRAVSMAPPAPPMPAMAQCGSDVPVAPRDVNRSSAPLLAIRGLRKS
                     FRGVRALDDVDLDVYAGEIVGVLGPNGSGKSTLINVVSGHFAADAGSIRFAGNELAGM
                     AAHRIARTGIARTYQIPRPFAHLSVRDNVALTAIFGAARCDRPQADAAALRWLAFTDL
                     LPRASALPGALNLHQTKFLELARALASQPRLLMLDEVLSGLTPAETDEAVELIESIRA
                     QGTTIVLVEHVMRVVTALSDRIVVLDQGRVLAEGVAADVMARHDVAVAYLGVAHA"
     gene            1796..2509
                     /locus_tag="VFO53_13485"
     CDS             1796..2509
                     /locus_tag="VFO53_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8877339.1"
                     /translation="MLDVRALRVHYGAAPALWDASLSIGDGELVCVIGPNGAGKTTLI
                     NAIAGLHRATSGQMTMDGCDLTTLPAHRFCAAGIALVPEGRRLFVRMSVRDNLAIGSY
                     LCRDRRAREASLDAVCALFPALHGKLDAPAGSLSGGQQQMVAIGRALMARPRLLLLDE
                     PSLGLSPLMVQAMFGAVRDVNASGTAVLLVEQNVAMALDIARRAYLLEEGRIVAHGST
                     AEVLEHPDLRRAYLGAVTR"
     gene            2557..3105
                     /locus_tag="VFO53_13490"
     CDS             2557..3105
                     /locus_tag="VFO53_13490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET8877340.1"
                     /translation="MSKPAVGIIVGSNRRESINRRLAKALAKLASDRLDFGWIGIDAL
                     PMYNQDLESDRPEPVRRFTAEVARFPALLFVTPEHNRSVPAVLKNALDWGSKPTDQNV
                     WRGKVAAITGTSFGAIGTAVGQQHLRQILAILGVLVMGGEAYISFRPEPVDDDGNFLD
                     ESKRAFCAAYMDRFAALVTKLA"
     gene            complement(3114..4250)
                     /locus_tag="VFO53_13495"
     CDS             complement(3114..4250)
                     /locus_tag="VFO53_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET8877341.1"
                     /translation="MRFPVVLMQLVLAAVVGYAAGRFAQNGAPERAPLPQSPPAATLP
                     APPAPSPTAVPPPATPENAAGAALSVSFRSAVERAASSVLTVHSGKSVARGPLGLGGR
                     ALLSEGLGSGVLIDADGYVVTNNHVVEGANELAVTLPDGSLLPTKLIGVDPDSDLALL
                     KIEASGLHPIAIGDLKSAAVGDVVLAVGNPLGVGQTVTQGIISALGRKGIGINPIENF
                     IQTDAAINPGNSGGALIDTSGRLIGINSVILSRGGGSEGIGFAIPIDIAQKVIASLKK
                     NGRVARGWLGISTTQAARGQPGALVVAVQRAAPADRAGIVPGDVIVGFGDQRIEQPDD
                     VAGATLELEPGTRVALEVVRDGKHRTLQVVLGTRPALRRDGTRR"
     gene            complement(4267..5007)
                     /locus_tag="VFO53_13500"
     CDS             complement(4267..5007)
                     /locus_tag="VFO53_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET8877342.1"
                     /translation="MAAEPHAAVHERRLAATTTLRVATWNIHGAVGGDGRYLPARVVG
                     VLHEIDADIVALQEVASEEAHTSFLADLGRDTGYHVVAGLLRQRHGSDFGNAVLSRFP
                     VRSVEHLDLAVDHYEPRGALDVCIDLGLRPLLRVVATHLGLRPGERREQVRRILAKVE
                     CESPHPTLLMGDLNEWYLWGRPLRWLHSHFREMPAAPRTFPARRPVFALDRIWVAPAG
                     SLRTLVRHATPLARSASDHLPLIAEIVV"
     gene            complement(4997..>6934)
                     /locus_tag="VFO53_13505"
     CDS             complement(4997..>6934)
                     /locus_tag="VFO53_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET8877343.1"
                     /translation="EFLNAVVAGQPDLHGYVLSWDFAMLYAMEREWLPIYKLDWRTHR
                     RLSFRLDDRHPVGASHHQKVIVIDDALAFVSGYDLTRCRWDTSAHRMADPRRVDHRGE
                     AYPPFHDVGVMVTGDCAAALGELARERWHRATGERPAQAVPGDAATLWPAGIVPAARD
                     VDVAISRTEPAFAGRAAVTEIRELHLDAIALARRHIFAENQYFTSRTITDAFVRRVAQ
                     NEGPEIAVLSPYMQSGWLEISTMGALRARIHHTLRAADLHRRYRLYCPALDWLDQREG
                     CLNVHSKVMIVDDALLMVGSANLSERSLGTDTECNLAIEAGDDPALAKLIAGLRDRLL
                     AEHLGRTAEEVARTLEREGSLHRAIETLGRPGARSLVAIEPRFDPALDALMPDQHVFD
                     PEQPLDPDRIAADLAPQDVRSGTRARLIGIAIGVAALACMAAAWRFTPLHEWLALDRL
                     VDIGVAISEQPWAPLAVLLAFVGAGLIAFPLLVLIAVTAMVFGPWLGPLYTLVGATMS
                     AALTFGIGRRLGRETVRKLAGTRVNDLSRRLAKRGLIAIAFVRMLPIAPFSIVNVVAG
                     ASHIRWSDFLLGTVIGLLPGIVTMTFFVDRAIAALRSPSPGTLGLLALALALIVALVW
                     VLRRMLRGHDRGDPAPAPHGS"
BASE COUNT         1039 a         2406 c         2418 g         1071 t
ORIGIN      
        1 gttcacgctg ctgatcgcgc tgctgctcgt tcgcccggga cgatcctgaa ggtcgaccgc
       61 acgatcgtcg gcatcgtcgc ggcgggcgcg gcactcgcga cgattccgtt cctgcgcctg
      121 ccggcgttct acgagtcgtt cctctacctc gtcctgcact ggatcgtcct cgcgacgtcg
      181 tggaacatcc tgtcgggcta ctccggctat ttttcgttcg ggcacggtgc gttctacggc
      241 gcgggcgtct acacgacggc ggtcctggcc ggcacgttcg gcgtgccgtt tctgtggacg
      301 ctgccggcgg cgggcatcgt cgcggcgctg ctcggcgtgg ggctcggcgc cgtcgtcttt
      361 cgcgtgcggc gcgtgcgcgg cgagctcttc gcgctgctga cgctcgccgt cacgttcgtg
      421 ctcgcgacga tcgtgcagaa cacgccgatc gacggcgggc ccggcgtcta cctgtcggcc
      481 gtcgccgtgc cggcggttgc acccaccgcg tcgggcgcgt tctacgtgat ggcgctggcg
      541 ctggccgtcg tcgcactcgt gctcgcgcgg gcgatcgcgc gctcgcgcct cgggctcggc
      601 ctcttcgcga tccacgacga cgaggacgcg gccgaggtga tgggcgtgcc gacgtaccgc
      661 tacaagctcg cggccttcgg catctcgtgt gcgctcgccg gcattgccgg cggcatccag
      721 gccctcttcg tttcgtacgt gaccgcgagc agcacgttca acatcacggt gccgctgacg
      781 gtcgtgctga tgtgcgtgct cggcggcacc cggcattggg cggggcccgc gctcggcgcg
      841 acggcaatca cgctgctgct ttacgcgtcg acggcggccg accaggcggt cgccggcaag
      901 gcgatcaccg gtgccattct cgtggcggcc gtgttgttca tgcccgacgg cgtcctcggt
      961 ctcgtgcgca ggcgaaagcg cgccgtgagc atggcgccgc cggcgccgcc catgccggcc
     1021 atggcgcagt gcgggtccga tgtgccggtc gcgcctcgtg atgtcaatcg ctcctcggcc
     1081 ccgctcctcg cgatccgcgg cctgcgcaaa tcgtttcgcg gcgtgcgcgc gctcgatgac
     1141 gtcgacctcg acgtgtacgc gggcgagatc gtcggcgtgc tcggtccgaa cggctcgggc
     1201 aagtcgacgc tgatcaacgt cgtcagcggc cattttgcgg ccgatgcggg cagcatccgc
     1261 ttcgccggca acgagctcgc cggcatggcg gcgcatcgca tcgcacgcac cgggattgcg
     1321 cggacctacc agatcccgcg cccgtttgcg cacctgtcgg tgcgcgacaa cgtggcgctc
     1381 accgcgatct tcggtgccgc ccgatgcgat cgcccgcagg ccgacgcggc ggcgctgcgg
     1441 tggctcgcgt tcacggacct gctgccgcgc gcgagcgcgc tgcccggcgc gctcaacctg
     1501 caccagacga agttcctcga gcttgcgcgc gcactcgcgt cgcagccacg actgctgatg
     1561 ctcgacgaag tgctgtcggg gctcacaccc gcggaaaccg acgaggccgt cgagctgatc
     1621 gaaagcatcc gtgcgcaggg gacgacgatc gtgctggtcg agcacgtgat gcgtgtcgtc
     1681 accgcacttt ccgatcgcat cgtcgtgctc gaccaggggc gtgtgctcgc cgagggcgtg
     1741 gcggccgacg tcatggcacg ccacgacgtc gccgttgcct atctcggcgt cgcgcatgct
     1801 tgacgtgcgc gcgctccgcg tgcactacgg cgccgcaccc gcgctgtggg acgcgtcgtt
     1861 gtcgatcggc gatggcgagc tcgtctgcgt catcgggccg aacggcgccg gcaagacgac
     1921 gctgatcaac gcgatcgcag gactgcacag ggcgacgtcc gggcagatga cgatggacgg
     1981 gtgcgacctc accacgctgc ccgcgcatcg cttttgcgcc gcgggcattg cgctggtgcc
     2041 ggagggccgg cggctgttcg tgcgcatgag cgtgcgcgac aacctcgcga tcggcagcta
     2101 tctgtgccgc gaccgtcgtg cgcgcgaagc ctcgctcgac gcggtatgcg cgctcttccc
     2161 ggcgttgcac ggcaagctcg acgcaccggc gggatccctc tccggcgggc agcagcagat
     2221 ggtggcgatc ggccgcgcgc tgatggccag gccgcgcctt ctgctgctcg acgagccgtc
     2281 gctcgggttg tcgccgctga tggtgcaggc gatgttcggc gcggtccgcg acgtcaacgc
     2341 gagcggcacg gcggtgctcc tcgtcgagca gaatgtcgcg atggcgctcg acatcgcgcg
     2401 ccgcgcgtac ctgctcgagg agggacgcat cgtcgcccac ggcagcaccg ccgaggtgct
     2461 cgagcatccc gatctgcgcc gggcctacct cggcgccgtg acccgatgag cttcggtcac
     2521 gatcccatcg acagaaaatc agcggaggca gcgacaatga gcaagcccgc ggtcggcatc
     2581 atcgtcggca gcaaccggcg cgaatcgatc aatcgccggc tcgcaaaggc gcttgcgaag
     2641 cttgcgtcag atcgcctcga cttcggctgg atcggcatcg atgcactgcc gatgtacaac
     2701 caggatctcg aatccgaccg acctgaaccg gtgcgccggt tcaccgccga ggtcgcgcgc
     2761 tttcccgcgc tgctcttcgt cacgcccgag cacaatcgct cggtcccggc ggtgctcaag
     2821 aatgcactcg actggggctc caagccgacc gaccagaacg tgtggcgcgg caaggtcgcc
     2881 gcgatcacgg gcacgtcgtt cggcgcgatc ggcaccgcgg tggggcagca gcatctgcgt
     2941 cagatcctcg ccatcctcgg cgtgctggtg atgggcggcg aggcgtacat ctcgtttcgc
     3001 ccggagccgg tcgacgacga cggcaatttc ctcgacgaga gcaagcgcgc gttctgcgcg
     3061 gcctacatgg atcggttcgc ggcgctggtg acaaagctcg cctgaccgcc gcgtcatcgg
     3121 cgcgtgccgt cgcgccgcag cgcgggccgc gtgccgagca ccacctgcag cgtccgatgc
     3181 ttgccgtcgc gcacgacctc gagcgccacg cgcgtgcccg gctcgagctc gagcgtcgca
     3241 cccgcgacat cgtcgggctg ctcgatacgc tggtcgccga agccgacgat cacgtcgccc
     3301 ggcacgatgc ccgcgcgatc cgccggtgca gcccgctgca ccgcgaccac cagtgcgccg
     3361 ggttgaccgc gtgccgcctg cgtcgtcgag atgccgagcc agccgcgggc cacgcggccg
     3421 ttcttcttga ggctcgcgat caccttctgc gcgatgtcga tcgggatggc gaacccgatg
     3481 ccttcggagc cgccgccgcg cgaaagaatc accgagttga tgccgatcag ccggccggac
     3541 gtgtcgatca gcgccccgcc ggagtttccg ggattgatcg ccgcgtcggt ctggatgaaa
     3601 ttctcgatcg ggttgatgcc gatgcccttg cgcccgagcg cgctgatgat gccctgcgtc
     3661 accgtctggc cgacgccgag cggattgccc acggcgagca ccacgtcacc gacggcggcg
     3721 ctcttgagat cgccgatcgc gatcggatgc aatcccgacg cctcgatctt gagcagtgcg
     3781 aggtcggaat cggggtcgac gccgatgagt ttcgtgggaa gaagcgaccc gtcgggaagc
     3841 gtcaccgcga gctcgttcgc gccttcgacg acgtggttgt tcgtgacgac gtagccgtcg
     3901 gcgtcgatca gcacgcccga gcccagcccc tcgctcagca aggcgcgtcc gccaaggccg
     3961 agcggcccgc gtgcgaccga ctttcccgaa tgcacggtca gcaccgacga tgcggcgcgc
     4021 tcgaccgcgc tgcggaagct caccgaaagt gcggcgccgg cggcgttctc gggcgtcgcg
     4081 ggaggtggca ccgccgtggg ggacggcgcg ggtggcgcgg gtaacgtcgc cgcgggtggc
     4141 gactgcggaa gcggcgcgcg ttccggcgcg ccgttctggg caaaccggcc cgctgcgtag
     4201 ccgacgacgg cggcgagaac caactgcatc agcacgacag gaaatcgcat ggctcctcga
     4261 ttttcttcag acgacgattt cggcaatcag cggcagatgg tcggaggcgc tgcgcgcaag
     4321 tggcgttgca tgacgcacga gcgtgcgcag cgacccggca ggcgctaccc agatccggtc
     4381 cagcgcgaac accggccggc gcgcgggaaa cgttcgcggc gcagccggca tctcgcgaaa
     4441 gtgcgaatgc agccagcgca gcggccttcc ccacaggtac cactcgttga ggtcgcccat
     4501 cagcagcgtc gggtgcggac tctcgcactc gaccttggca aggatgcgcc gcacctgctc
     4561 gcgccgctcg cccggacgca acccgagatg ggtcgccacg acgcgcaaca gcggccgcag
     4621 gccgaggtcg atgcaaacgt cgagtgcgcc acgcggctcg taatgatcga cggcaagatc
     4681 gagatgctcg acgctgcgca cgggaaatcg cgacagcacg gcgttgccga agtcgctgcc
     4741 gtggcgctgg cgcagcagcc ccgcaacgac atggtaaccg gtgtcgcgcc cgaggtcggc
     4801 gaggaagctc gtatgcgcct cctcggaggc cacttcctgc agtgcgacga tgtcggcgtc
     4861 gatttcgtgc agcacgccga ccacccgcgc cggcagatat cggccgtcgc cgcccaccgc
     4921 accatggatg ttccacgtcg cgacacgcag cgtggtcgtg gcggcgagcc gcctttcgtg
     4981 cacggcggca tgcggctcag ctgccatgcg gagcgggcgc gggatcgccg cggtcgtgtc
     5041 cgcgcagcat gcggcgcagc acccagacga gtgcgacgat cagcgcgagc gcaagcgcga
     5101 gcagtccgag tgtgccgggg ctggggctgc gcagcgcggc gatcgcgcga tccacgaaga
     5161 acgtcatcgt gacgatgccg ggcagaaggc cgatgacggt gccgagaagg aaatcgctcc
     5221 agcgaatatg cgatgcgccc gcgacgacgt tgacgatcga aaacggcgcg atcggcagca
     5281 tgcgcacgaa cgcgatcgcg atcagtccgc gcttggcgag acgccgcgac aggtcgttga
     5341 cgcgcgtccc ggcgagcttc ctcaccgtct cccggccgag cctgcggccg atgccgaacg
     5401 tcagcgcggc cgacatcgtc gcgccgacca gggtgtagag cggcccgagc cagggcccga
     5461 agaccatcgc ggtgaccgcg atcagcacca gcagcggaaa cgcgatgagt cccgcaccga
     5521 cgaacgcgag cagcacggca agcggcgccc agggctgctc gctgatcgca acgccgatgt
     5581 cgacgagccg atcgagcgcg agccactcgt gcagcggcgt gaaccgccac gcggcggcca
     5641 tgcacgcgag ggccgccacg ccgatggcga tgccgatgag gcgcgcccgc gttccgctgc
     5701 gcacgtcctg cggcgcgaga tcggccgcga tgcgatcggg atcgagcggc tgctcgggat
     5761 cgaacacgtg ctgatccggc atcagcgcgt cgagcgccgg gtcgaaccgc ggctcgatgg
     5821 cgacgagcga gcgcgcgccc ggccgcccaa gcgtttcgat cgcgcgatga aggctccctt
     5881 cgcgctcgag cgtccgcgcg acttcctccg cagtgcgccc gagatgctcg gcgagcaggc
     5941 ggtcgcgcag tcccgcgatg agcttcgcca gcgccggatc atcgcccgcc tcgatcgcga
     6001 gattgcattc ggtgtcggtg ccaagcgagc gctcggacag gttcgccgag ccgaccatga
     6061 gcagcgcatc gtcgacgatc atcaccttgc tgtgcacgtt gaggcagcct tcgcgctggt
     6121 cgagccagtc gagcgcggga cagtacaggc gatagcggcg gtgaaggtcg gccgcacgca
     6181 gcgtatggtg aatgcgcgcg cggagcgccc ccatcgtaga aatttcgagc cagccgcttt
     6241 gcatgtacgg ggacagcacg gcgatttccg gtccctcgtt ctgtgccacg cgacgcacga
     6301 acgcgtcggt gatcgtgcgt gacgtgaaat actggttctc cgcgaatatg tgacggcgcg
     6361 cgagtgcaat ggcatcgagg tgcagctcgc ggatctcggt gaccgcggcg cgcccggcga
     6421 acgcgggctc ggtgcgcgat atcgcaacgt cgacgtcgcg cgcggcggga acgatgccgg
     6481 caggccacag cgtcgccgcg tcacccggca ccgcttgcgc cggccgctca ccggtcgcgc
     6541 gatgccagcg ctcgcgcgcg agctcgccga gcgccgccgc gcagtcgccc gtcaccatca
     6601 cgccgacgtc gtggaacgga ggataggcct cgccgcgatg atcgacgcgc cgtggatcgg
     6661 ccatgcggtg cgcgctcgta tcccagcggc agcgcgtgag gtcatagccg ctcacgaacg
     6721 ccagcgcgtc gtcgatgacg attaccttct gatgatgcga cgcgccgacc ggatggcggt
     6781 cgtcgaggcg aaacgaaagg cggcggtgcg tgcgccagtc gagcttgtag atcggcagcc
     6841 actcgcgttc catcgcgtag agcatcgcga agtcccacga cagcacgtag ccgtgcaggt
     6901 cgggctggcc cgcgaccacc gcattgagga attc
//
LOCUS       DASSVX010000317         6826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_530633, whole genome shotgun sequence.
ACCESSION   DASSVX010000317 DASSVX010000000
VERSION     DASSVX010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530633"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFO53_13510"
     CDS             complement(<1..317)
                     /locus_tag="VFO53_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET8877344.1"
                     /translation="MALPDSMRQIEISQPGAPEVMHVARAPLPQPRADEVLIEVAYAG
                     VNRPDCAQRAGTYPPPPDASPILGLEVAGRIVARGDAVRDWNVGDEVCALAPGGGYAE
                     YCTT"
     gene            complement(375..1079)
                     /locus_tag="VFO53_13515"
     CDS             complement(375..1079)
                     /locus_tag="VFO53_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8877345.1"
                     /translation="MLEIANLRAAYGDAQVLRGVSMRVDKGRVVALLGRNGMGKTTLI
                     RSVMGMAPPRVLEGSIAFHGRELRGLRPHQVAQQRIGLVPQGRRLFASLTVTEHLTML
                     GRHAGSGSGWNVGRLFELFPRLGERRRHRGHQLSGGERQMLAVARALTLNPELLLMDE
                     PSEGLAPVMVQHLERIVVELRRMGYAILLVEQNLYSALAVADEAYIMETGSIVHHGSV
                     EVLREDRATMHRFLGI"
     gene            complement(1072..1767)
                     /locus_tag="VFO53_13520"
     CDS             complement(1072..1767)
                     /locus_tag="VFO53_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8877346.1"
                     /translation="MDGISLGVREGERRAIIGPNGAGKTTLFNAIAGEVPVSGGSVRF
                     AGRDVTHLRTHRRAALGMGRTYQITNLFPGLTVEENVRLAAQSLDRRKFSMLGAAAPK
                     GAEAERIEATLTTSGLAARRRDPTRTLSYGEQRQLEFALALASKPALLLLDEPAAGLS
                     AAERLVMAATISALPRTLTLVLIEHDIDLALSLVDHVTCMHNGTVLVEGSPDAIRANE
                     RVQQVYLGKPHDA"
     gene            complement(1818..3086)
                     /locus_tag="VFO53_13525"
     CDS             complement(1818..3086)
                     /locus_tag="VFO53_13525"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877347.1"
                     /translation="MNRRKFIRSASAMIAANTVAAAGPLLPSAIAQTGPIRIGLLAPL
                     TGVVAAGGKEMVEGFNLYWDQHGRKIAGRDIEIIVEDDASNPDTALQKARRLVEQRNV
                     HMLIGDLLANTGLAVAEYVKGNGVPYFIPIIAADDLTQRKRIPNVIRIAGYSASQMPR
                     PLADYAYRKKGYRKVVTISQDYTFGYEQCGGFCQTFSELGGSVVQQFWQPLNTADFSP
                     YLAQIQNLKPDVVYVMETGADANRFIQQYANFGLKGTIPMLGCMNLTDQSVIRTLGAE
                     CEGIVSSAQFCEGSDAPATRNFVAEYEKAFNKLPSLYGFSMYSGAMWMAAGMTAVNGN
                     VEDRPKFLDAIRKVVLNDSPLGRPVRLDAYGNPIYDVYIREVKRRPDGKYWNVPLETV
                     PDVSQFGKYDPETYLKQASYSRTFQGIKKS"
     gene            complement(3111..4049)
                     /locus_tag="VFO53_13530"
     CDS             complement(3111..4049)
                     /locus_tag="VFO53_13530"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8877348.1"
                     /translation="MTSRARATIGFVCAALLAAVPFVVPGYKTSLVIEMLIFATLAMS
                     IDLLAGFAGRVSLGHGAIFGIATYVVLYYVTTAGGSPWVAMLLGVAAATVAAVIFALL
                     AIRTSGVYFLLLTLALGMIVWGMCLRWTSVTGGENGLRGVVAPAWLAHQPVFYFFILT
                     VTALMTWLMWRFARSPFGLTLQGIRESESRMRALGYNVPLHLFIAFTVSGFFAGLAGT
                     LYAFFNSFVSPSTVALAQSVEGLLMVIVGGVGTLFGSLVGAAVIITLENLVSFYTERW
                     PMVLGLMFIVTMIFAPEGLLGWGRAALARRRSRNAS"
     gene            complement(4046..4903)
                     /locus_tag="VFO53_13535"
     CDS             complement(4046..4903)
                     /locus_tag="VFO53_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8877349.1"
                     /translation="MSMTVFQVLNGATFAALLFIVASGFTLIFGLMRIVNLAHGALYL
                     VGGYIGLVVASRSGSFLLALAAAGVAIAAIGLLTYTLLLRFVRGNVLRQVLLTLGLAL
                     VLDDLALVIWGGDAFTVQVPEMLRGPLRIGALAYPKYRLFLLLFSMLVFIALTLLLRR
                     TRLGALIRAGVDDAEMVDAMGIDIQRVFIYTFMLGSALAGIGGVLGGAFLTLYPTADS
                     DILVFSLAVVIIGGRGSLAGTAIGSLFVGMLATFGQVWFPELAYFVIFGPMALLLAFR
                     PLGLFGRAA"
     gene            complement(5025..5891)
                     /locus_tag="VFO53_13540"
     CDS             complement(5025..5891)
                     /locus_tag="VFO53_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET8877350.1"
                     /translation="MLPTERLDYSPIVSRTPWRLPDGARLAVWVIVNVEEWDITQPMP
                     RTVLTPPAGGTPAPDIPNWAWHEYGNRIGFWRLLEILDRFGIAAALAINGACIASYAP
                     IAAAAQTRGWEFIGHGYTQKNMQKVDDERAAIRATADAIARFTGKRPRGWLGPGLTET
                     WETPDILAEEGYAYVCDWVLDDQPVTLQTRTGTIVNIPYTQECNDVAMMLIQHHAARE
                     YADRAIDQFEQLYEDARESARVMALVVHPYIMGAPHRARHFARALEVIHGRNGAVFST
                     GEQILDWYLATK"
     gene            complement(5964..6593)
                     /locus_tag="VFO53_13545"
     CDS             complement(5964..6593)
                     /locus_tag="VFO53_13545"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET8877351.1"
                     /translation="MNLGIDHVCAAVVGIDLHRGHLDPAVATMPLDAPAAGRVVAANR
                     KLFDACRSRGVPVVHLVTRYRDSDEIRANPFWRTRAENPDNPRRHVLKHNLTHLPGCT
                     IMPGLADARDFVVDTKKRYDCFVGTDLDFLLRAHGINTLIVTGVNTNSCVLSTVAAAC
                     SKDYAVIVASDCVDTMDGAALHEAALACIRTAFGFVMTSDEAIGLLDRK"
BASE COUNT         1236 a         2211 c         2270 g         1109 t
ORIGIN      
        1 gtcgtgcaat actcggcgta gccgccgccg ggcgccagcg cgcaaacctc gtcgccgacg
       61 ttccagtcgc gcacggcgtc accgcgcgcg acgatgcgtc cggcaacttc gagtccgagg
      121 atcggcgacg catcgggtgg cggcggatag gtcccggcgc gctgcgcgca atcgggccgg
      181 ttgacgccgg catacgcgac ttcgatcagc acctcgtccg cgcgcggttg cggcagcggc
      241 gcgcgcgcga cgtgcatgac ctccggtgca ccgggttggg agatttcaat ctgacgcatc
      301 gagtcgggca gtgccatggt cacgttcctg caagcgaaag cacgattcta cgagcctcgc
      361 tcggcccgcg agcgtcagat gccgaggaag cggtgcatcg tcgcgcgatc ctcgcgcagc
      421 acctcgacgc tgccgtgatg cacgatgctg ccggtttcca tgatgtacgc ctcgtcggcg
      481 accgccagcg cgctgtacag gttctgctcg acgagcagga tcgcgtaacc catccgccgc
      541 agctcgacca cgatgcgctc gagatgctgc accatcaccg gcgcgagacc ttcggatggc
      601 tcgtccatca gcagaagctc gggattgagc gtcaacgcac gcgccacggc cagcatctgc
      661 cgctcgccgc ccgaaagctg gtgcccgcga tgccgccggc gctcgccgag gcgggggaac
      721 agttcgaaca gacgcccgac attccatcct gaaccgctgc ccgcatgccg gccgagcatc
      781 gtcaggtgct cggtcacggt cagcgacgca aacagacgcc gtccctgcgg cacgagaccg
      841 attcgctgct gcgcgacctg gtgcggtcgc aggccgcgca actcgcggcc gtgaaacgcg
      901 atgctgcctt ccagcacgcg gggcggcgcc atgcccatga ccgagcggat gagcgtcgtc
      961 ttgcccatgc cgttgcgccc cagcagggca acgacacggc ccttgtcgac gcgcatcgac
     1021 acgccgcgca acacctgcgc atcgccgtac gccgcgcgca gattcgctat ctcaagcatc
     1081 gtgcggcttg cccaggtaca cctgctgcac gcgctcgttc gcccggatcg catccgggct
     1141 tccttcgacg agcaccgtcc cgttgtgcat gcacgtgacg tggtcgacga ggctcagcgc
     1201 cagatcgata tcgtgttcga tcagcacgag cgtcaacgtg cgcggcaacg ccgaaatggt
     1261 cgccgccatg acgagccgct cggccgccga cagtcctgcc gcgggctcgt cgagcagcag
     1321 caacgcgggc ttgctcgcaa gcgccagcgc gaattcgagt tgccgttgct ccccatagga
     1381 aagcgtgcgc gtcggatcgc ggcgccgggc ggccagcccg ctcgtcgtca gcgtcgcttc
     1441 gatgcgttcg gcttccgcac ctttcggcgc cgccgcgccc agcatcgaaa acttccgcct
     1501 atccaggctc tgcgcggcga ggcgcacgtt ttcctcgacc gtgagcccgg gaaacaggtt
     1561 ggtgatctgg tacgtgcggc ccattcccag cgccgcacgg cggtgcgtgc gcagatgcgt
     1621 gacgtcgcgc ccggcgaagc gcacgctgcc gccggaaacc ggcacttcgc cggcaatggc
     1681 attgaacaac gttgtcttgc ccgcgccgtt cgggccgatg atcgcccggc gctcgccttc
     1741 gcgcacgccg agcgaaatgc cgtccacggc gcgcagcgcg ccgaagctga cggccagagc
     1801 gtcgagctgc agcaggttca ggatttcttg atgccctgga acgtgcggct gtaggaagcc
     1861 tgcttgagat acgtctcggg atcgtatttg ccgaactgcg agacgtcggg aaccgtttcc
     1921 agcggcacgt tccagtactt gccgtcgggg cgccgcttca cctcgcggat gtagacgtcg
     1981 tagatcggat tgccataagc gtcgagccgc accgggcggc cgagcggcga atcgttcagc
     2041 acgaccttgc gtatcgcatc gaggaatttc gggcgatcct cgacgttgcc attgacggcc
     2101 gtcatgccgg cggccatcca catcgctccc gaatacatcg aaaagccgta gagcgacggc
     2161 agcttgttga acgccttctc gtactcggcg acgaaattcc tggtggccgg cgcgtcgctg
     2221 ccttcgcaga attgcgccga cgagacgatg ccttcgcact cggcgccgag cgtgcggatc
     2281 accgattgat cggtcaggtt catgcagccg agcatcggga tcgtgccctt caagccgaag
     2341 ttcgcgtatt gctggatgaa gcggttggcg tcggcgcccg tttccatcac ataaacgacg
     2401 tccggcttca ggttctggat ctgcgccagg tacgggctga aatcggccgt gttgagcggc
     2461 tgccagaatt gctgcacgac cgatccaccg agttcgctga atgtctggca gaagccaccg
     2521 cactgctcgt agccgaacgt gtaatcctgc gagatcgtca ccaccttgcg gtagcccttc
     2581 ttgcggtacg catagtcggc cagcggtcgc ggcatctggc ttgccgaata accggcgatg
     2641 cggatgacgt tgggaatgcg cttgcgctgc gtcaggtcat cggcggcgat gatcgggatg
     2701 aagtacggca cgccgttgcc cttgacgtac tcggcaaccg cgaggccggt gttggcgagg
     2761 aggtcgccga tcagcatgtg gacgttgcgc tgctcgacga gacgccttgc cttctgcaac
     2821 gcggtatcgg gattgctggc gtcgtcctcg acgatgattt cgatgtcgcg tccggcgatc
     2881 ttgcggccgt gctggtccca gtagaggttg aacccctcca ccatttcctt gccgcccgcg
     2941 gcgacgacgc cggtaagcgg cgcgagcagt ccgatgcgga tgggaccggt ttgcgcaatc
     3001 gcggacggga gcagcggccc cgcagcggcg accgtgttcg ccgcgatcat ggcgctggcg
     3061 gatctgatga atttgcggcg attcatgacg actcctcctt gttagaaata tcaagacgca
     3121 ttccgggaac ggcgtcgcgc gagcgcggcg cgtccccagc ccagcaagcc ttcgggggca
     3181 aaaatcatcg ttacgatgaa catgagtccg agcaccatcg gccagcgctc cgtgtagaag
     3241 ctgacgaggt tctccagcgt gatgatgacc gcggcgccga ccagcgagcc gaacagcgtg
     3301 ccgacgccgc cgacgatcac catcagcaag ccttcgaccg actgtgcgag cgcaaccgtc
     3361 gacggactga cgaagctgtt gaagaacgcg tacagcgtac ccgcgaggcc ggcgaagaaa
     3421 ccggagacgg tgaaggcgat gaacaggtgc agcggcacgt tgtagccgag cgcccgcatg
     3481 cgcgactcgc tctcgcggat gccctgcagc gtgaggccga acggcgaacg cgcaaaacgc
     3541 cacatgagcc acgtcatcag cgcggtgacc gtcaggatga agaaatagaa cacgggctgg
     3601 tgcgcgagcc acgcgggtgc gacgacgccg cgcaggccat tttccccgcc ggtcaccgac
     3661 gtccagcgca ggcacatgcc ccagacgatc atgcccagcg ccaatgtcag cagcaggaaa
     3721 tacacacccg acgtgcggat cgccagcaac gcgaagatca ccgccgcgac ggttgcagcc
     3781 gccaccccga gcagcatcgc gacccacggc gagccgccgg ccgtcgtcac gtagtagagc
     3841 acgacgtaag tcgcgatgcc gaagatcgcg ccgtgaccga gcgatacacg tcccgcgaag
     3901 ccggcgagca ggtcgatcga catggcgagc gtggcgaaga tcagcatttc gatgacgagg
     3961 ctcgtcttgt agccgggcac gacgaacggc acggccgcga gaagtgctgc gcacacgaag
     4021 ccaatcgtcg cccgtgcacg cgacgtcatg cggcgcgacc gaacaacccg agcgggcgaa
     4081 acgcgagcag cagcgccatc ggtccgaaaa tcacgaaata ggcgagctcg ggaaaccaca
     4141 cctggccgaa ggtggcgagc atgccgacga acaggctgcc gatggcggtg ccggcaaggc
     4201 tgccgcgtcc gccgatgatc accacggcca ggctgaacac gaggatatcg gagtcggccg
     4261 tcggatagag cgtgagaaac gcgccgccca gcacgccgcc gatgccggcc agcgccgagc
     4321 ccagcatgaa ggtataaatg aacacgcgct ggatgtcgat gcccatggca tcgaccattt
     4381 cggcgtcgtc gacgccggcg cggatcagcg cgccgagacg cgtgcggcgc agcagaagcg
     4441 tcagcgcgat gaagaccagc atgctgaaaa gcagcaggaa cagccggtat ttcggatagg
     4501 ccagcgcgcc tatccgcaac ggcccgcgca gcatctccgg cacctggacc gtgaacgcgt
     4561 cgccacccca gatgacgagc gccaggtcgt cgagcacgag cgcgagtccc agcgtgagca
     4621 gcacctgccg cagcacgttg ccccggacga agcgcagcag cagcgtgtac gtcagcaatc
     4681 cgatggcggc aatggcaacg ccggcggcgg cgagcgccag caggaagctg ccgctgcggc
     4741 tcgcgacgac gaggccgatg tagccgccga ccaggtaaag cgcgccgtgg gcgagattca
     4801 cgatgcgcat cagcccgaag atcagcgtga agccgctcgc cacgatgaac agcagcgcgg
     4861 caaacgtcgc gccgttcaac acctggaaga cggtcatcga catggcggcc cgtgcaggga
     4921 agtgagctcc ggcacgcttg tatgattccc ccacgcttgc ggcttcgccg ctgcgctgct
     4981 gcccgagggg gcgcaattcg cgccttgagg cggctctgca gcgctcattt cgtcgcgagg
     5041 taccagtcga ggatctgctc tccggtgctg aatacggcgc cattgcggcc gtgaatgact
     5101 tccagcgcgc gtgcgaagtg tcgcgcgcgg tgcggcgcac ccatgatgta cggatgcacg
     5161 acgagcgcca tcacccgtgc cgattcgcgg gcatcctcgt agagctgctc gaactggtcg
     5221 atcgcccggt cggcatattc gcgtgcggca tgatgctgga tcagcatcat cgcgacgtcg
     5281 ttgcattcct gcgtgtaggg aatgttgacg atcgtgcccg tgcgcgtttg cagcgtgacc
     5341 ggctggtcgt cgagcaccca gtcgcagacg taggcatagc cttcctcggc gaggatgtcg
     5401 ggcgtttccc acgtttccgt caaaccgggc ccgagccagc ctcgcggtcg cttgccggtg
     5461 aaccgcgcga tcgcgtcggc ggtggcacgg atcgccgcgc gctcgtcgtc gactttctgc
     5521 atgtttttct gcgtgtaccc atggccgatg aactcccagc cgcgcgtttg cgcggctgcc
     5581 gcgatcggtg cgtagcttgc gatgcatgcg ccgttgatgg cgagcgcggc cgcgatgccg
     5641 aagcggtcga ggatctcgag caaccgccag aacccgatcc ggttgccgta ttcgtgccac
     5701 gcccagttcg ggatgtccgg cgccggcgtg ccaccggccg gcggcgtcag cacggtgcgc
     5761 ggcatcggct gcgtgatgtc ccattcctcg acgttgacga tcacccagac ggccaaacgt
     5821 gcgccgtccg gcagccgcca tggcgtacgc gaaacgatcg gtgaatagtc gagccgctcg
     5881 gtcggcagca tggaagcggg gtcgggaacg gatttcgtgg aggtgggtct tgcgcaccag
     5941 gcggatgaaa ctcgtccgtg acgctatttt cgatcgagga ggccgatcgc ctcatcactg
     6001 gtcatcacga agccgaatgc cgtacgaatg catgcgagcg ccgcctcgtg cagggcggcc
     6061 ccgtccatcg tgtccacgca atcgctggcc acgatcacgg cgtagtcctt cgaacacgcg
     6121 gcggcgacgg tcgacagcac gcagctgttc gtgttgacac cggtgacgat gagcgtgttg
     6181 atgccgtgcg cccgaagcag gaaatcgagg tcggtgccga cgaagcagtc atagcgcttc
     6241 ttcgtgtcga cgacgaagtc gcgcgcatcg gcgaggccgg gcatgatggt gcagcccggc
     6301 agatgcgtca ggttatgctt gaggacgtgc cggcgcgggt tgtccggatt ctcggcgcgt
     6361 gtgcgccaga acggattggc gcggatctcg tcgctgtcgc ggtagcgcgt tacgagatgc
     6421 actaccggaa cgccgcgcga gcggcacgcg tcgaacagct tgcggttggc ggcgacaacg
     6481 cgcccggccg cgggagcgtc gagcggcatc gtcgcgacgg caggatcgag gtgaccgcga
     6541 tgcaggtcga tgccgacgac agcggcgcat acgtggtcga tgccgagatt catggaattg
     6601 gcggcctggg tgggtggcgg acagtggccc gcagcatgtt agcgccatcg ccgctcacgt
     6661 caacccggtg aggaattcgg tgtcacggca acgcgctaat gtccggtctt agcaaagtag
     6721 aaatgtccgg tacgttccag cctccagcaa gcagggggcg cgatggagac cggaatgagt
     6781 cagcaggaat tggagcgtgt agaggtgatt gcgttgcgcc agtcgg
//
LOCUS       DASSVX010000318         6582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_264202, whole genome shotgun sequence.
ACCESSION   DASSVX010000318 DASSVX010000000
VERSION     DASSVX010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264202"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..440)
                     /locus_tag="VFO53_13550"
     CDS             complement(114..440)
                     /locus_tag="VFO53_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET8877352.1"
                     /translation="MMKNQLAGLMKQAQQMQENMQRAQEELARTEVEGESGAGLVKIV
                     MTCRHDVKRVSIDPSLLGEDRDMLEDLVAAAFNDALRRAEATSQEKLGSLTAGMPLPP
                     GFKLPF"
     gene            complement(437..2152)
                     /gene="dnaX"
                     /locus_tag="VFO53_13555"
     CDS             complement(437..2152)
                     /gene="dnaX"
                     /locus_tag="VFO53_13555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET8877353.1"
                     /translation="MSYQVLARKWRPRAFGELVGQEHVVTALTNALTRGRLHHAYLLT
                     GTRGVGKTTLARILSKSLNCEQGVSATPCGECAACRDIDAGRFIDLLELDAASNTGVE
                     NMREILDNARYAPTAGRYKVYLIDEVHMLSKGAFNSMLKTLEEPPEHVKFVLATTDPQ
                     KIPVTVLSRCLQFNLTPLPRTLIVDRLAHILGAESVAFDAAALPLLAAGAQGSLRDAL
                     SLLDQAIAYGGGEVREPAVRAMLGVVDRDYLYRLVDALLGNDGAALLAEADRLGAAGH
                     AFAPALDELALLFHRIAVVQAVPGAPGGDDGERVADYAARMTPESVQLAYQIAVQGRA
                     DLALAPDEATGFSMTLLRLLAFEPAREGEATSTSTPREAARTVPADKVLGVDRNVVAT
                     RAADAPTKAKAVVPPATREPANGAPGHDSGDAQHLALPESNADWPAFVAGLRLIGMAA
                     QLAAQSELKSVRGNALSLAIPATHKHLADKAYADKLKAALEAATGRKLLLAFEIGESS
                     DGSLAAIARREREQARAEGEAAFRSEPFVRDLVTRFDATVRPETIEPAPREVPPPSST
                     RQETR"
     gene            complement(2265..2363)
                     /gene="ffs"
                     /locus_tag="VFO53_13560"
     ncRNA           complement(2265..2363)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFO53_13560"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2648..4060
                     /gene="mltF"
                     /locus_tag="VFO53_13565"
     CDS             2648..4060
                     /gene="mltF"
                     /locus_tag="VFO53_13565"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HET8877354.1"
                     /translation="MRDRVFARTTIIGLAGLVLLAFSGCNEPLAPRLPASPGINVVVR
                     PGPSTWFIGPNGQPSGFDHDLLTRFARERGVPLNVVYADGAAALLAKISHGEAQLGAG
                     GLFPSANGNDVPGASPALAWSSGYHAVEPVLIYNVDGFRPRRWDDLDKASVAYPAHTG
                     MDRQLAAVRTAHPEVRWQPVDVVSADALIAQVADGTISYAVVASSDATVARNIYLDFD
                     VAFAAGPPREFAWAVAPGYPELRDALNAFFARLRKDGTLARLTERYFAESPPVERIDA
                     GVFQDRIRKALPEWRRTFIDAQAKSGIEWRLLAAIAYQESQWDAGATSETGVRGLMQL
                     TEDTARALGVADRLDPKSAAIGAAKYLADLKRKLPSRIAEPDRTWLALAAFNIGIGHL
                     EDARVLAQRQKLNPDLWSDVRKVLPLLAEPEYYVTLRNGYARGGMPVAFVGRVRGYYD
                     ILLRTEDAGRPRLHASLAQR"
     gene            4088..4927
                     /locus_tag="VFO53_13570"
     CDS             4088..4927
                     /locus_tag="VFO53_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET8877355.1"
                     /translation="MRHSRPIAYHWRMAGHPLRLVAACLLALASTIAHSQQSVFLDEL
                     TTTEVQAQVRAGKTTVLIPIGGTEQSGPAIVLGKHNVRVKVLSGKIAAALGNALVAPV
                     IAYVPEGGIDPPTSHMRFAGTISIPVASFESVLDAAARSLRAHGFRDVVFLGDHGGYQ
                     QSEARVAARLDREWASTPARAHALTEYYRVTETEYPAALAAHGFTKAEIGTHAGLADT
                     SLSLAVDPALVRSRELASGKDLDAAHGVYGDPRRATAELGRLGVDAIVQRSVESIRRA
                     TRR"
     gene            5099..6154
                     /locus_tag="VFO53_13575"
     CDS             5099..6154
                     /locus_tag="VFO53_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HET8877356.1"
                     /translation="MPPVVDPANLYSETSAAHLSPAVRNALPRVYVPNVQSDDVYVID
                     PGTLKVVNRFRVGLNPQHVVPSWDLSTLWVANNAEGTTKGSLTPIDPATGKPGKPIAV
                     DDPYNMYFTPDGRSAIVVAEAHKRLDFRDPRTMALQWSLDVPDCGGINHADFSIDGRY
                     AIFTCEFAKGLAKIDFVDRKVLGYLTLSKGGMPQDVRISPDGKLFYVADMMADGVFIV
                     DGATFREVGFIATGKGTHGLYPSRDGRKLYVTNRGSNRIHGPAHGKGSVSVVDFATQK
                     VDVTWPIPGGGSPDMGNVSADGKQLWLSGRYDNVVYVFDTTSGAVQKIPVGKEPHGLA
                     VWPQPGRYSLGHTGNMR"
     gene            complement(6160..>6582)
                     /locus_tag="VFO53_13580"
     CDS             complement(6160..>6582)
                     /locus_tag="VFO53_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HET8877357.1"
                     /translation="VQIVRPENSYAALEELTGNAETILQKLALPYRVMALCTGDIGFA
                     AAKTYDLEVWLPGQNAYREISSCSNCEAFQARRMQARFRNDKGKPELVHTLNGSGLAV
                     GRTLVAVMENYQREDGSIDVPAALLPYMNGVDVIRRSR"
BASE COUNT         1044 a         2274 c         2206 g         1058 t
ORIGIN      
        1 cggccttcgg tccgacgccg gggaggcagc gcagggcctg cgcaagctcg tccaggctgc
       61 cgagcgattc ggacgccgca cgggcgccgc catcgcgcgc gttcacggcg aaatcagaac
      121 ggaagtttga aacccggcgg cagcggcatg ccggccgtga ggctgccgag cttttcctgc
      181 gacgtcgcct cggcgcggcg caatgcatcg ttgaacgccg ccgcgacgag atcttccagc
      241 atgtcgcggt cctcgccgag aagcgacgga tcgatcgata cgcgcttcac gtcgtggcgg
      301 caggtcatca cgattttcac gagccccgcg cccgactccc cctcgacttc ggtgcgcgcg
      361 agctcttcct gcgcgcgctg catgttttcc tgcatctgct gcgcctgctt catcaggccg
      421 gccaactggt tcttcatcat cgcgtttcct gcctcgtgga ggatgggggc ggcacctcgc
      481 gcggcgccgg ctcgatcgtt tccggacgca ccgttgcgtc gaatcgggtg accagatcgc
      541 ggacgaacgg ctcgctgcga aacgcggctt cgccttccgc gcgcgcctgc tcgcgctcgc
      601 ggcgcgcgat ggcggcaagc gagccgtcgc tgctctcgcc gatctcgaac gcgagcagga
      661 gcttgcgccc ggtcgctgct tccagcgcgg ccttgagctt gtcggcgtaa gccttgtcgg
      721 caagatgctt gtgcgtggcc gggatggcaa gcgacagcgc gttgccgcgc accgacttca
      781 actcggattg cgcggcgagc tgcgccgcca tgccgatcag ccgcagtccg gcaacgaacg
      841 ccggccagtc ggcgttcgat tcgggcagcg ccaggtgctg cgcgtcgccg gaatcgtgac
      901 ctggggcgcc gttcgccggt tcccgcgtcg cgggcggtac gaccgccttc gccttcgtcg
      961 gcgcatcggc agcacgcgtc gcgacgacgt tgcgatcgac gccgagcacc ttgtccgcgg
     1021 gcacggtgcg cgccgcttcg cgcggcgtcg acgtgctcgt tgcctcgccc tcgcgtgcgg
     1081 gctcgaacgc gagcagccgc aaaagggtca tcgaaaagcc ggtcgcttcg tcgggggcca
     1141 gcgcaagatc ggcgcgaccc tgcaccgcga tctgataggc aagctgcacg ctttcgggcg
     1201 tcatccgcgc cgcgtaatcg gcaacccgtt cgccgtcatc cccgcccggc gcgccgggaa
     1261 cggcctggac gaccgcgata cgatggaaca aaagcgcgag ctcgtcgagg gcgggcgcga
     1321 aggcgtggcc ggcggcgcca aggcggtcgg cctccgcgag cagcgccgca ccgtcgttgc
     1381 cgagaagcgc gtcgaccagc cggtacaggt aatcccggtc gacgacgccg agcatcgccc
     1441 gcacggccgg ctcgcggacc tcgccgccgc cgtacgcgat ggcctggtcg agcagcgaca
     1501 gcgcgtcgcg cagcgatcct tgtgcacccg cggcaagaag cggcagcgct gcggcgtcga
     1561 acgcgaccga ttcggcgccg agaatgtgcg cgagccgatc gacaatcagc gtgcgcggca
     1621 gcggcgtcag gttgaactgc aggcagcgcg acagcacggt gaccggaatc ttctgcgggt
     1681 cggtcgtcgc gagcacgaac ttgacgtgtt cggggggctc ctcgagtgtc ttcagcatcg
     1741 agttgaacgc gcccttcgac agcatgtgca cctcgtcgat caggtacacc ttgtaacgcc
     1801 ccgcggtcgg cgcataacgc gcgttgtcga ggatctcgcg catgttctcg acgccggtgt
     1861 tggacgcggc gtcgagctcg agcaggtcga tgaagcggcc ggcgtcgatg tcgcggcagg
     1921 cggcacactc gccgcacggc gtcgccgaca cgccctgctc gcagttgagg ctcttcgaca
     1981 ggatgcgcgc gagcgtcgtc ttgccgacgc cgcgggtccc ggtcaggagg taggcatggt
     2041 gcagccggcc gcgcgtcagc gcgttcgtga gcgcggtcac cacgtgctcc tgcccgacga
     2101 gctcgccgaa cgcacgcggc cgccatttgc gtgcgaggac ttgatagctc atcgaagcgt
     2161 gctgtgccgt gggccgaaaa cgccggctat ttcgcctcgg gtcgcgcgaa gacagggcgc
     2221 gagtccggga gaacgcacag cttacggact ggcaaaggaa taggggtggc gagccgttcc
     2281 cccggcactt gcactcaatc gctgtggctg cttccttccg gacctgacca gattcacgac
     2341 cgtgcaatgc ggggcgaccc gccgttgatc ggggcgccgt ttgcgcgaag ccgccattat
     2401 accgggcacg acggggtcgg accggctgag cggtcgaaaa gccacccacg agcggctcga
     2461 cgcgcggcca ggcggtcgct tggccatgca gttcgaaggg ttataatcgg ccgcgtcgcc
     2521 aggatgcctt cgcacccgcg accaccatgg agtttccgca gcccgtggca aggctgcgcc
     2581 aatgtacggc ggcaaagatc gccgcaaacc ttcgagcacg cgcccggcaa cggggcggtg
     2641 gagaaccatg cgcgatcgag tgtttgcccg cacgaccatc atcggcctcg ccgggctcgt
     2701 gctgcttgcg ttctccggct gcaacgaacc cttggcgccg cgcctgcccg cctcgccggg
     2761 gatcaacgtg gtcgtccggc ccggaccgtc gacctggttc atcggcccca acggccaacc
     2821 cagcggcttc gaccacgatc tgctgacccg ctttgcgcgc gaacgcggcg tgccgctcaa
     2881 cgtcgtctat gccgacggcg cggcggcgct gctcgccaag atcagccatg gcgaggcgca
     2941 gcttggtgcg ggcggcctgt tcccgtcggc caacggcaac gacgtaccgg gtgcgagccc
     3001 cgcccttgcc tggagcagcg ggtatcacgc ggtggaaccg gtgctgatct ataacgtcga
     3061 cggtttccgc ccccggcgct gggacgatct cgacaaggcg agcgtcgcct accctgcaca
     3121 taccggcatg gaccggcagc tcgccgccgt ccgtaccgcg catcccgagg tgcgctggca
     3181 gccggtggac gtggtctccg ccgatgcgct gatcgcgcag gtcgccgacg gcaccatcag
     3241 ctatgcggtg gtcgcctcga gcgacgcgac ggtcgcgcgg aacatctatc tcgatttcga
     3301 cgtcgcgttc gcggccgggc ccccgcggga atttgcctgg gccgtcgcgc ccggctaccc
     3361 cgagcttcgc gacgcattga acgccttctt cgcccggctg cgcaaggatg gcacgctcgc
     3421 ccgattgacg gaacgctatt tcgccgaaag tccgcccgtc gagcgcatcg acgcaggcgt
     3481 gttccaggat cgcatccgca aggcgctgcc cgaatggcgc aggacgttca tcgacgcgca
     3541 ggcgaaaagt ggaatcgaat ggcgcctgct tgccgcgatc gcttaccagg aatcgcaatg
     3601 ggacgcgggc gcgacgagcg aaaccggtgt gcgcggcctc atgcagttga ccgaagacac
     3661 ggcgcgcgca cttggcgtcg ccgaccgtct cgatccgaag tccgcggcga tcggcgcggc
     3721 gaagtacctc gccgacctca agcggaagct gccgtcgcgc atcgccgagc ccgatcgcac
     3781 ctggcttgcg ctcgcggcgt tcaacatcgg catcggccac ctcgaggatg cgcgggtgct
     3841 tgcccagcgg cagaagctca atcccgacct ctggagcgat gtgcgcaagg tgttgccgct
     3901 cctcgccgag cccgagtact acgtgacgct caggaacggc tatgcgcgag gcggcatgcc
     3961 cgttgccttc gtcgggcgcg tgcgcgggta ctacgacatc ctgctgcgca ccgaggacgc
     4021 gggcagaccg cgcctgcacg caagcctcgc gcaacgctga gcgaacgcgg cgcctgcaaa
     4081 ggcgtccatg cggcattcgc ggccgatcgc ctaccattgg cggatggccg gtcacccgtt
     4141 acgcctcgtt gccgcatgtc ttctcgcgct cgcgtcgacg atcgcgcatt cgcagcaaag
     4201 cgttttcctc gacgagctga ccaccaccga agtgcaggcg caggtgcgcg ccggcaagac
     4261 gaccgtgctg atcccgatcg gcggcaccga gcagagcgga ccggcgatcg tcctcggcaa
     4321 gcacaacgtg cgggtgaagg tgctgtcggg aaagatcgcc gcggcgctcg gtaacgcgct
     4381 ggtcgcgccg gtgatcgcct acgtgcccga aggcggcatc gatccaccca cctcgcacat
     4441 gcgcttcgcc ggcacgatct cgatcccggt tgcctcgttc gaaagcgtgc tcgacgcggc
     4501 ggcacgaagt ctgcgcgccc atggctttcg cgacgtcgtc ttcctcggag atcacggcgg
     4561 ctatcagcag agcgaggcgc gggttgcggc gcggctcgac cgcgaatggg cgagcacgcc
     4621 ggcgcgcgcg cacgcgctca ccgagtacta tcgggtgacc gaaacggagt atcccgccgc
     4681 gctcgccgcg cacgggttca ccaaggccga gatcggcacg catgccggac tcgccgatac
     4741 ttcgctgtcc cttgccgtcg atcccgcgct cgtgcgttca cgcgagctcg cctccggcaa
     4801 ggatctcgac gcggcgcacg gcgtctacgg cgatccgcgc cgcgccacgg cggagctcgg
     4861 gcgcctcggc gtcgacgcca tcgtgcagcg ctcggtcgaa tcgatccggc gcgcaacgcg
     4921 gcgctgatgc gcctcttctg taacccatcc acatcaggaa agtgacttcg tgaagcttcg
     4981 cgtttccctt gcttcgatcc ttgcgctgct gctcgcgtgc gtgatcgcgt cgccggccgg
     5041 cgcacagcct ggcgcaaccc ctgcaccgtc ggcatccgca gtcacgacgc tgcccggcat
     5101 gccgcccgtc gtcgatccgg ccaacctcta cagcgaaacg tcggcagcgc acctgagccc
     5161 cgccgtgcgc aacgcgctgc cccgcgtgta cgtgccgaac gtgcagtcgg acgacgtcta
     5221 cgtgatcgac cccgggacgc tcaaggtcgt gaaccggttc agggtgggcc tcaacccgca
     5281 gcacgtcgtc ccgtcgtggg atctgtcgac gctatgggtc gcgaacaacg ccgaggggac
     5341 gacgaagggc agcctgacgc cgatcgatcc tgcgaccggc aagccgggta agccgattgc
     5401 ggtcgacgat ccctacaaca tgtacttcac gcccgacggc cgctcggcaa tcgtcgtcgc
     5461 cgaggcgcac aagcggctcg acttccgcga tcccaggacg atggcgctgc aatggtcgct
     5521 cgacgttccg gactgcggcg gcatcaatca cgccgacttc tcgatcgacg gacgctacgc
     5581 gatcttcacc tgcgaattcg cgaagggtct cgcgaagatc gacttcgtcg atcgcaaggt
     5641 gctcggctac ctgacgctgt cgaagggcgg catgccgcag gacgtgcgca tcagccccga
     5701 cggcaagctg ttctatgttg cggacatgat ggccgacggc gtgttcatcg tcgacggcgc
     5761 gacattccgc gaagtgggct tcatcgcgac cggcaagggc acgcatgggc tctatcccag
     5821 ccgcgacggg cgcaagctct acgtcaccaa tcggggctcg aacaggatcc acggaccggc
     5881 gcacggcaag ggcagcgtgt cggtggtcga tttcgcgacg cagaaggtcg acgtcacgtg
     5941 gcccattccc ggcggcggca gccccgacat gggaaatgtc agcgccgacg gcaagcagct
     6001 gtggctatcg gggcgctacg acaacgtcgt ctacgtgttc gacacgacca gcggcgcggt
     6061 gcagaagatt ccggtgggca aggagccgca cgggctcgcc gtgtggccgc agcccgggcg
     6121 ctactcgctc gggcataccg gcaacatgcg ctagtttttt tatcggctgc ggcggatgac
     6181 gtcgacgccg ttcatgtacg gcagcagcgc cgcggggacg tcgatcgagc cgtcttcgcg
     6241 ctgatagttc tccatcaccg caaccagcgt gcgcccgacg gcgagccccg agccgttcag
     6301 cgtgtgcacg agctcgggct tgcccttgtc gttgcgaaag cgcgcctgca tccggcgcgc
     6361 ctggaacgcc tcgcaattcg agcatgacga gatctcgcga taggcgttct gaccgggaag
     6421 ccagacctcg aggtcgtatg tcttggcggc cgcgaagccg atgtcgccgg tgcaaagcgc
     6481 catcacgcga tagggcagcg caagcttctg caggatcgtc tcggcgttcc cggtcagctc
     6541 ttcgagtgcc gcgtacgaat tctccggacg cacgatctgc ac
//
LOCUS       DASSVX010000319         9818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1214037, whole genome shotgun sequence.
ACCESSION   DASSVX010000319 DASSVX010000000
VERSION     DASSVX010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9818
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214037"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1290)
                     /locus_tag="VFO53_13585"
     CDS             complement(124..1290)
                     /locus_tag="VFO53_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HET8877358.1"
                     /translation="MTDAVSFDFGLGETIDMLRASVREFAAAEIAPRAAVIDRDNAFP
                     ADLWRKLGAMGLLGITVEEDYGGTGLGYLAHIVAMEEISRASASVGLSYGAHSNLCVN
                     QIRRNGSDAQKRRYLPKLVSGEHVGALAMSESGSGSDVVSMRLRAGRRGEVYVLNGTK
                     MWITNGPDANTLVVYAKTDPQAGARGITAFIVEKGMRGFTTAQKLDKLGMRGSNTCEL
                     VFDDCEVPAANVLGDEGRGVNVLMSGLDYERTVLAGGSLGIMAACFDAVLPYVHERRQ
                     FGQPIGEFQLMQGKLADMYTTMNACRAYVYAVGRACDDGRTTRKDAAGAILYAAEKAT
                     WMASEAIQCLGGNGYINEFPTGRLWRDAKLYEIGAGTSEIRRMLIGRELFSESE"
     gene            complement(1287..1730)
                     /locus_tag="VFO53_13590"
     CDS             complement(1287..1730)
                     /locus_tag="VFO53_13590"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET8877359.1"
                     /translation="MALIGARLEALRPGYCALTLTPRPETSQQHGYVHAGILATLVDS
                     AGGYAGYTLFPADSSVLTVEFKLNLLAPAEGDRIIAEGFVVKSGRTLTITRGEVHAER
                     NGERTLVAIMQQTLMTMRGKSDARREDRDTSGADAPPAASVERGS"
     gene            complement(1833..2258)
                     /locus_tag="VFO53_13595"
     CDS             complement(1833..2258)
                     /locus_tag="VFO53_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET8877360.1"
                     /translation="MPATNRHAAPPGARATYTISELAREFALTTRAIRFYEDCGLLTP
                     ARDKRTRVYRERERVRLKLILRGKRLGLALSEIGELLDLYEVRRNEHAQLTAFLVLLA
                     ERRMRLLQQREDIEAVLAEIDGIERECRRRLKAEARAES"
     gene            2324..3367
                     /locus_tag="VFO53_13600"
     CDS             2324..3367
                     /locus_tag="VFO53_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8877361.1"
                     /translation="MNDSLSHALLQIDGEPPPPGDAREIAPGIRWLRMPLPFALDHIN
                     LWLIDEDDGCTLVDCGYGDATTRALWQRHFETTLAKRPLARIVVTHCHPDHVGNAEWL
                     AARYGCAVTMTQGEYLAAHALRGGHAGYGSADAQALFARHGMSSGHVAAFAARGNRYR
                     RGVPEVPHSFARIIDNDVLTLGGLPWRVIAGYGHSAEHASLAAVDARLLIAGDMLLPR
                     ISTNVSVWPGEPDADPVQRFLDSLAAFAALPPDTLVLPSHGVPFRGIGARVAQLAAHH
                     AARLAELQRWLAAARVPQSAQDVIPVLFRRALDVHQRFFAMGEAIAHLNNLWRHRLAE
                     RHVGDDGAIRFTA"
     gene            3414..5237
                     /gene="phaC"
                     /locus_tag="VFO53_13605"
     CDS             3414..5237
                     /gene="phaC"
                     /locus_tag="VFO53_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HET8877362.1"
                     /translation="MATPPPTTTSTSSTPDAAGFDPVKLAESLATAAEKSAKLMGEFV
                     ARNAGKQAAVANDELGLGKAFMELAAKMLANPARIAEGQMRLWRDYMTLWQSSMLRMM
                     GAQALPVAEPAQGDKRFRDAGWHEHFLFDYMKQSYLITARWLHEQVASVEGLSEPAKK
                     KVQFFTRQYIDALAPSNFALTNPEVFRETVSSGGQNLVRGLNNLLDDIERGNGQLRIS
                     MTDPKAFELGVNIATTPGKVIYQNALMQLIQYEPATAKVRKRPLLIIPPWINKYYILD
                     LREKNSFIRWAVSEGITVFVVSWVNPDATLAHKDFEDYLVDGPLAALDAIRDATGEPD
                     ANVIGYCLGGTLLAATLGWLAAKKQKRIASATFMTALVDFTAAGELEVFIDEAQVASL
                     EKKMQERGYLEGSEMANTFNLLRANDLIWSFVINNYLLGRDPFPFDLLHWNCDSTRMP
                     ARMHSYYLRNMYMGNKLREPGALTLAGVPIDLSKVTVPTYFVSAIEDHIAPWKTTYAG
                     PGLIQGKKRFVLSGSGHIAGMINPPAANKYGYWTNESLADDPDEWFAGAAQHAGSWWS
                     DWRAWIEPFLDREVAARVPGNGRLPVIEAAPGSYARLRADS"
     gene            complement(5287..6999)
                     /locus_tag="VFO53_13610"
     CDS             complement(5287..6999)
                     /locus_tag="VFO53_13610"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HET8877363.1"
                     /translation="MRAAATGLAVLFAGTLALAAHAQADDPLPTRVGRLASVQGAIYH
                     SASDGGDDDWVPIGLNYPVAQGDNLWADHGARAEVDYGGGQFRLAGDTNVHVSRLDER
                     QLALFVASGRVIVRVRYLQPDDSVRVDTATTQIALERPGLYRIDVEATAQQTTLLVRE
                     GEAAVATAAGAQHVLPGQIALVRETANATADIRSGGGLDGFDTWSAERDRVYEQPREN
                     AYVSREMVGQNDLDGHGQWQAYPEYGAVWFPTVDPEWAPYRFGQWTWLPGFGYTWVDS
                     APWGYAPFHYGRWAYIGGRWGWCPGAFVARPTWAPALVAWYGDGSFSGAPVYGWVPLG
                     WGEPFVPAWRGCGSRCYARYNRPYAVNVAERPGARPTRYANWTVPGGATAVPAGTLTT
                     GRPVAPNRVPVATYRPVAPALTAAPPAVKPAPVRPGAVRAGNGAPLPAAAFAARVARP
                     ASPPPQSRTAPLPAPPTRPAPGLSAPVLPERSLRPVPVPPPAPAPAPIRVPAPAMPLP
                     RMPAPSVPGVAPAPPAAVVPLPPRAPAPIVAVPPPRAPAPSAVPAPAAPVAPVAPRPP
                     AVQN"
     gene            complement(7183..7950)
                     /locus_tag="VFO53_13615"
     CDS             complement(7183..7950)
                     /locus_tag="VFO53_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8877364.1"
                     /translation="MTPGVFITGASSGIGEALARHYAARGAALGLYARRPGELDRVAA
                     SLAPAVVATFAGDVRDPVALAAAANDFIARFGTPGIVIANAGVSRGTSTEHAEDLAAF
                     REVFDTNVLGLVNAFQPFIGAMRATGRGTLVGIASIAGFRGVPGSGAYSASKAAAIAY
                     LESLRVELRGSGISVVTVCPGYIATPMTAHNPYRMPFLMQADTAARRIANAIARRKRF
                     YVLPWPMALAGRVFRALPRPLYDRVFANAPRKPRRPG"
     gene            complement(7947..8594)
                     /locus_tag="VFO53_13620"
     CDS             complement(7947..8594)
                     /locus_tag="VFO53_13620"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HET8877365.1"
                     /translation="MSIPQLRFIGRGLIALFACALFAGAASAALVEGKNYERMANPQP
                     VETGKKIEVIEFFSYGCPHCAALEPFLDTWLEKLPPDVAFRRVPVMFHSDWVQLAKVF
                     YTLDAMGQVKLSPEVFKAIHQTGTRLSDKKTFLDWAASKGLDRKKVEEVFDSFAIDGR
                     VKRAMQLAQEYRIQSVPSVIVDGKFVTSSDRVGTHANLPAAIDELVAKARAERKS"
     gene            complement(8680..9321)
                     /locus_tag="VFO53_13625"
     CDS             complement(8680..9321)
                     /locus_tag="VFO53_13625"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET8877366.1"
                     /translation="MARTPQPRGALGGTLLGVFIGIIVGLGMAASVAYWLMKNNPALP
                     MPTFSRDANEPARDAAKVAKADDKPRFDFYKILPGVEEPKVQAAPDRGDRAVVEQAKA
                     RVPDKPAERAAPALQPAAEKLASAADTTARPVKPNDRFWLQAGSFSTVPDAENLRARL
                     ALAGWEASVQEGALPDKSVRYRVRLGPYDNADELGRMKSELARRGFDVAVIHY"
     gene            complement(9418..>9818)
                     /locus_tag="VFO53_13630"
     CDS             complement(9418..>9818)
                     /locus_tag="VFO53_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HET8877367.1"
                     /translation="SQSEDNPVYYVQYAHARVASVLKQAGVPFDTARAALRGADLSSL
                     ESRYETPLLRRLADFPDEVALAAREFAPHQLTFYLKDLAQEFHSYYNAERFLVDDPRI
                     RSARLALIVAVGQVLRNGLTLLGIGAPDAM"
BASE COUNT         1568 a         3376 c         3368 g         1506 t
ORIGIN      
        1 tggtcatggc attacccttg attcgctaat cggggtcaga gccgtaatta ttcggcgcac
       61 cgggatgcgc cgaataatta cggctctgac cccgattaca ccggccttgt cgcgacgcgg
      121 gtgtcattct gattcgctga aaagctcgcg accgatcagc atgcgccgga tttcggacgt
      181 tcccgcgccg atctcgtaga gcttggcgtc gcgccacagc cgtcccgtgg gaaactcgtt
      241 gatgtagccg ttgccgccga ggcactggat cgcctcgctc gccatccacg tcgccttctc
      301 cgccgcgtag aggatggcgc ccgccgcgtc cttgcgcgtc gtccggccgt cgtcgcatgc
      361 gcggccgacc gcgtagacgt aggcgcggca ggcgttcatc gtcgtgtaca tgtcggcgag
      421 cttgccttgc atcaactgga actcgccgat cggctgtccg aactggcgcc gctcgtgcac
      481 gtagggcagc accgcatcga agcacgcggc catgatgccg agcgagccgc ccgcgagaac
      541 ggtccgctcg taatcgaggc cgctcatcag cacgttcacg ccgcggcctt cgtcgccgag
      601 cacgttcgcg gcgggcactt cgcaatcgtc gaacacgagc tcgcacgtgt tcgagccgcg
      661 catgccgagc ttgtcgagct tctgcgcggt ggtgaagccg cgcatgccct tttcgacgat
      721 gaatgcggtg atgccgcgcg cgcccgcctg cggatcggtc ttcgcgtaga cgacgagcgt
      781 gttcgcgtcg ggcccgttgg tgatccacat cttcgtgccg ttcagcacgt agacctcgcc
      841 gcgtcgcccg gcgcgaaggc gcatcgacac gacgtcggat cccgagcccg actcgctcat
      901 cgcgagcgcg ccgacatgct cgccgctgac gagcttcggc aggtagcggc gcttctgcgc
      961 gtcgctgccg ttgcgccgga tctggttgac gcagaggttc gagtgcgcgc cgtacgacag
     1021 gccgaccgac gccgaggcac gcgaaatctc ctccatggcc acgatgtgcg cgaggtagcc
     1081 gaggccggtg ccgccgtagt cttcctcgac ggtgatgccg agcagcccca tggcgccgag
     1141 cttgcgccag aggtcggcgg ggaacgcgtt gtcgcgatcg atgaccgcgg cgcgcggcgc
     1201 gatttcggcg gccgcgaact cgcgcaccga cgcacgcagc atgtcgatgg tctcgccgag
     1261 cccgaaatcg aacgacactg catcagtcat gaaccccgct ccacgcttgc agcagggggt
     1321 gcatctgcgc cgctcgtgtc acggtcctcg cgccttgcgt cggacttgcc gcgcatcgtc
     1381 atcagcgtct gctgcatgat cgccaccagc gtcctttcgc cattgcgctc ggcatgcacc
     1441 tcgccgcgcg tgatcgtcag tgtacgaccg gatttgacga cgaagccctc cgcgatgatg
     1501 cgatcgccct ctgcgggtgc gagcaggttc agcttgaatt cgacggtcag caccgagctg
     1561 tcggccggga acagcgtgta gcccgcgtag ccgcccgccg aatcgacgag ggtcgcgagg
     1621 atgcccgcat gcacgtagcc gtgctgctgc gacgtttccg gtcgcggtgt cagcgtcagc
     1681 gcgcaatagc cgggccgcaa cgcctcgagg cgcgcgccga tcagcgccat cgcgccctgg
     1741 cgcgcgaagc tgtcacggat ccgctcggag aaagcgtcga tcgtcacgcc ggaattctcc
     1801 atgacgttta cgtaaacgtc aatctgcccg gttcagcttt cagcgcgcgc ctcggccttc
     1861 aggcggcgcc ggcattcgcg ctcgatgccg tcgatttcgg cgagcaccgc ctcgatgtcc
     1921 tcgcgttgct gcagaaggcg catccggcgc tcggcgagaa gcaccaggaa cgccgtcagc
     1981 tgcgcgtgct cgttgcgccg cacctcgtag aggtcgagca gttcgccgat ctcggatagc
     2041 gcgagtccga gccgcttgcc gcgcaggatc agcttgagcc gcacgcgctc gcgctcgcgg
     2101 tagacgcgcg tgcgcttgtc gcgcgccggc gtcagcagtc cgcaatcctc gtagaaccgg
     2161 atcgcgcgcg tcgtgagcgc gaactcgcgt gcgagctccg agatcgtgta cgtcgcacgc
     2221 gcgcccggcg gggcggcgtg gcggttcgtg gcgggcatgc aaatgcgtgg tgcgaagcgc
     2281 acggcgggca tggcggcgcg ggggggcacg ataccatacc cgcatgaacg attcgctgtc
     2341 gcacgccctt ctccagatcg acggggagcc gcctccgccc ggcgatgcac gcgagatcgc
     2401 gcccggcatc cggtggctgc gcatgccgct gccgttcgcg ctcgaccaca tcaatctgtg
     2461 gctgatcgac gaagacgacg gctgcacgct cgtcgactgc ggttatggcg acgcgacgac
     2521 gcgcgcgctg tggcagcgcc acttcgagac cacgctcgca aagcggccgc tcgcacgcat
     2581 cgtcgtcacg cactgccacc cggatcatgt cggcaatgcc gagtggctcg cagcacgcta
     2641 cggctgcgcc gtgacgatga cgcagggcga gtatctggcc gcgcacgcgc tgcgcggcgg
     2701 gcatgcgggc tacggttcgg ccgacgcgca ggcgctcttc gcgcgccacg gcatgtcgtc
     2761 cgggcatgtc gccgcattcg ccgcgcgcgg caatcgttat cggcgcggcg tgcccgaagt
     2821 gccgcactca ttcgcgcgga tcatcgacaa cgacgtgctg acgctcggcg ggctgccatg
     2881 gcgcgtcatc gcgggctacg gccattcggc cgagcatgcg tcgctcgcgg ccgtcgatgc
     2941 gcgcctgctc atcgccggcg acatgctgct gcctcggatc agcacgaacg tctccgtgtg
     3001 gccgggcgag cccgacgccg atccggtgca gcggttcctg gactcgctcg ccgcgttcgc
     3061 ggcgctgccg cccgacacgc tggtgctgcc ctcgcacggc gtgccgtttc gcggcatcgg
     3121 cgcgcgcgtt gcgcaactcg ccgcgcatca tgccgcgcgg ctcgccgagc tgcagcgatg
     3181 gctcgccgcg gcccgtgtgc cgcagtcggc gcaggatgtc attccggtgc ttttccggcg
     3241 cgcgctcgac gtgcaccagc gcttcttcgc catgggcgag gcgatcgcgc atctcaacaa
     3301 cctgtggcgc catcgcctcg ccgagcgcca cgtcggcgac gacggcgcga ttcgcttcac
     3361 cgcgtaatat tgcgcggttg ctctcccctc tcgcatttcc ttcctcgaac acgatggcga
     3421 cacctcctcc gaccacgacc tccacctcga gcacgcccga cgccgccgga ttcgacccgg
     3481 tcaagctcgc cgaatcgctc gccaccgccg ccgagaaaag cgcaaagctg atgggcgaat
     3541 tcgttgcgcg caatgcaggc aagcaggcgg ccgtcgcgaa cgacgagctc gggctcggca
     3601 aggcgttcat ggagctcgcc gcgaaaatgc tcgccaaccc ggcgcgcatc gccgaggggc
     3661 agatgcgcct gtggcgcgat tacatgacgc tgtggcagtc gtcgatgctg cgcatgatgg
     3721 gagcgcaggc gctgccggtc gccgaacctg cgcagggcga caagcgcttc agggatgccg
     3781 gctggcacga gcacttcctg ttcgactaca tgaagcagtc gtacctcatc accgcgcgct
     3841 ggctgcacga gcaggtcgca agcgtcgagg gcctgtcgga gccggcgaag aaaaaggtgc
     3901 agttcttcac ccgccagtac atcgacgcgc tcgcgccgtc gaacttcgcg ctgaccaatc
     3961 cggaggtgtt ccgcgagacg gtgtcgagcg gcggccagaa cctggtgcgc ggcctcaaca
     4021 atctgctcga cgacatcgag cgcggcaacg gtcagctgcg catttcgatg accgacccga
     4081 aggcgttcga gctcggcgtc aacatcgcca ccacgccggg caaggtcatc tatcagaacg
     4141 cgctgatgca gctgatccag tacgagccgg ccaccgcgaa ggtgcgaaag cggccgcttt
     4201 tgatcattcc gccgtggatc aacaagtatt acatcctcga tctgcgcgag aagaactcgt
     4261 tcatccgctg ggcggtgagc gaagggatca cggtgttcgt cgtctcgtgg gtcaaccccg
     4321 acgcaacgct cgcgcacaag gacttcgagg attacctggt cgacggcccg ctcgcagccc
     4381 tcgatgcgat ccgcgacgcg accggcgagc ccgacgcgaa cgtgatcggc tattgcctcg
     4441 gcggaacgct gctcgccgca acgctcggct ggctcgccgc gaaaaagcag aagcgcatag
     4501 cgagcgcgac gttcatgacg gcgctggtcg atttcaccgc ggcgggcgag ctcgaggtgt
     4561 tcatcgacga ggcccaggtc gcatcgctcg agaagaagat gcaggagcgc ggctatctcg
     4621 aaggcagcga gatggcgaac acgttcaacc tgctgcgcgc caacgacctc atctggtcgt
     4681 tcgtgatcaa caactacctg ctcgggcgcg acccgttccc gttcgacctt ctgcactgga
     4741 actgcgactc gacgcggatg cccgcgcgca tgcacagcta ttacctgcgc aacatgtaca
     4801 tggggaacaa gctgcgcgag ccgggcgcgc tgacgctcgc cggcgtgccg atcgacctgt
     4861 cgaaggtcac ggtgccgacg tacttcgtat ccgccatcga ggaccacatc gcgccgtgga
     4921 agacgacgta cgccggcccg ggactgatcc aggggaagaa gcggttcgtg ctgtccggct
     4981 cgggacacat cgccggcatg atcaatccgc cggccgccaa caaatacggc tactggacga
     5041 acgaatcgct ggccgacgac cccgacgaat ggttcgctgg cgccgcgcag cacgcgggct
     5101 cgtggtggag cgactggcgt gcatggatcg agccgtttct cgaccgcgag gtggcggcgc
     5161 gcgtgccggg caacggcagg ctgccggtca tcgaagcggc acccggctcg tacgcgcgtc
     5221 ttcgcgcgga cagctgaacg acggttcgtc aagcgatacg gcgcggcgtc cggcacggcc
     5281 ggactgctag ttctgcacgg cgggtggcct cggcgccacc ggggccaccg gggccgcggg
     5341 cgcaggaacg gcggagggtg ccggcgcacg cggcggcggc acggcgacga tcggcgccgg
     5401 cgcgcgcggc ggcaggggaa cgaccgcggc cggcggcgcc ggcgcgacac cgggcaccga
     5461 aggcgccggc attcgcggca gcggcatcgc gggcgccggc acgcgaatgg gcgcaggcgc
     5521 cggcgccggt ggcggcaccg ggaccggccg cagcgagcgc tccggcagca cgggcgcaga
     5581 caagcccggt gccggacgcg ttgggggcgc gggcagcggc gcagtacgcg actggggcgg
     5641 cggcgatgcc ggacgcgcga cgcgcgcagc aaacgcggcc gcgggaagcg gagcgccgtt
     5701 cccggcgcgc acggcacccg gccgaacggg cgcaggtttg accgccggcg gcgccgccgt
     5761 aagcgcgggc gcgaccggcc ggtaagtggc cacggggacg cgattgggcg ccaccgggcg
     5821 cccggttgtc agcgtgccgg ccggcaccgc cgtggcaccg cccggaaccg tccaattcgc
     5881 gtaacgcgtc ggccgcgcgc cgggccgttc ggcgacgttg accgcgtacg gccggttgta
     5941 gcgcgcgtaa cagcgcgatc cgcatccgcg ccatgccggc acgaacggct cgccccagcc
     6001 gagcggcacc cacccgtaca cgggcgcgcc tgaaaacgag ccatcgccgt accaggcgac
     6061 gagtgcaggt gcccacgtcg gccgcgcgac gaacgcgccg ggacaccagc cccagcgccc
     6121 gccgatgtac gcccagcggc cgtaatggaa cggcgcatag ccccatggcg cgctgtcgac
     6181 ccacgtgtag ccgaagccgg gcaaccacgt ccactgcccg aagcgatacg gcgcccattc
     6241 gggatcgacg gtcgggaacc atacggcacc gtattcggga taggcctgcc attgcccatg
     6301 gccgtcgagg tcgttctgcc ccaccatctc gcgcgacacg tacgcgtttt cacgcggctg
     6361 ctcgtacacc cgatcgcgct cggcactcca ggtgtcgaag ccgtcgagtc ccccgccgct
     6421 gcggatgtcg gccgtcgcgt tcgccgtttc gcgcacgagc gcaatctgtc cgggaagcac
     6481 gtgctgcgcg ccggcggccg tcgcgaccgc ggcctcgccc tcgcgcacga gcagcgtcgt
     6541 ctgctgtgcg gtcgcctcga cgtcgatgcg atagagaccc ggccgctcga gcgcgatctg
     6601 cgtcgtcgcg gtatcgacgc gtaccgaatc gtcgggttgc aggtagcgga cgcgaacgat
     6661 gacgcggccc gacgcgacga aaagcgcgag ttgccgctcg tcgaggcgcg acacgtgcac
     6721 gttcgtgtcg cccgcgagcc gaaactggcc gccgccgtag tcgacttcgg cgcgggcacc
     6781 gtggtcggcc cacaggttgt cgccctgtgc caccggataa ttgagaccga tcggcaccca
     6841 gtcgtcgtca ccgccatcac tggccgagtg atagatcgcg ccctgcacgc tggcgagccg
     6901 gccgacacgc gtcggcaatg gatcgtcggc ctgggcgtgc gcggcgagcg ctagcgtgcc
     6961 cgcgaacagc acggcgagcc ccgttgcggc ggcgcgcacg aagcgcgagc gttcggtgcg
     7021 agcgcagatc gcagtgcagt gcgccgagga tggaaccgcg tgcatgatgt ggtcgagaac
     7081 gtccacgcgc gcccgcccgt tgaccggaag cgccgcagcg aaggcaacga ttgcgcggtc
     7141 acgcccgtcg tgcggttgcc gcggtagcgc gccgtatcgt gatcagccgg ggcgtcgcgg
     7201 cttgcgcggc gcattggcga aaacacggtc gtagaggggc cgcggcagcg cgcggaacac
     7261 gcggcccgcg agcgccatcg gccacggcag cacgtagaag cgcttgcgcc gcgcgatggc
     7321 attcgcgatc ctgcgtgccg ccgtatcggc ctgcatcagg aaaggcatcc gatagggatt
     7381 gtgcgcggtc atcggcgtcg cgatgtagcc ggggcagacc gtgacgacgc tgatgccgct
     7441 tccacgcaac tcgacgcgca ggctttcgag gtacgcgatc gccgcggcct tcgacgcgct
     7501 gtaggcaccc gacccgggaa ccccgcggaa gccggcgatg ctcgcgatgc cgacgagcgt
     7561 tccgcgtccc gtcgcgcgca tcgcgccgat gaacggctga aacgcgttga cgaggccgag
     7621 cacgttcgta tcgaacacct cgcggaaagc ggccaggtcc tcggcgtgct cggtcgacgt
     7681 gccgcgcgaa acgccggcgt tggcgatcac gatgccgggc gtgccgaatc gcgcgatgaa
     7741 gtcgtttgcc gccgccgcga gcgccaccgg gtcacgcacg tcgcccgcga acgtcgcaac
     7801 gacggcgggg gcgagcgatg ccgcgacgcg gtcgagctcg cccggccttc gcgcatacaa
     7861 gccgagtgcc gcaccccgcg ccgcgtaatg acgcgcaagc gcttcgccga tgcccgacga
     7921 ggcgccggtg atgaagacgc cgggcgtcag ctcttgcgct cggcgcgcgc cttcgcgaca
     7981 agctcgtcga tcgccgcggg caggttcgcg tgggtgccga cgcgatcgga cgacgtcacg
     8041 aacttgccgt cgacgatcac gctggggacg gactggatcc ggtattcctg cgcgagctgc
     8101 attgcgcgct tgaccctgcc gtcgatggcg aacgaatcga acacctcctc gaccttcttg
     8161 cggtcgagac ccttgctcgc cgcccagtcg aggaaggtct tcttgtcgga aagccgcgtg
     8221 cccgtctggt gaatggcctt gaacacctcg ggcgaaagct tgacctgtcc cattgcatcg
     8281 agcgtgtaga acacctttgc gagctggacc cagtcggaat ggaacatcac cgggacgcgc
     8341 cggaacgcga cgtcgggggg cagcttctcg agccaggtgt cgagaaacgg ctcgagcgcc
     8401 gcgcaatgcg gacagccgta cgagaagaat tcgatcacct cgatcttctt gcccgtctcg
     8461 acgggctgcg gattggccat gcgctcgtag ttcttgcctt cgacgagcgc agcacttgcc
     8521 gcacccgcga acagcgcgca agcgaagagc gcaatcaggc cgcggccgat gaaacgcagt
     8581 tgcggaatgc tcatggagac tcctggaaaa tgaagaagct tgggctcgcg caggcacgcg
     8641 cgagttccgg ccgcgctgcc gctgcccgcc gaatggacgt caatagtgga tgaccgcgac
     8701 gtcgaagccg cgccgcgcga gctccgactt catgcgtccg agctcgtcgg cgttgtcgta
     8761 gggtccgagg cggacgcgat agcgcacgct cttgtccggc agcgcgcctt cctgcacgct
     8821 cgcctcccaa ccggcgaggg cgagccgcgc gcgcaggttc tcggcgtcgg gcaccgtcga
     8881 gaacgatccc gcctgcagcc agaagcgatc gttcggcttg acgggcctcg ccgtcgtgtc
     8941 ggccgcggac gcgagcttct ccgcagcggg ctggagcgcg ggcgccgcac gctcggccgg
     9001 cttgtccggg acccgtgcct tcgcctgctc gacgacggcg cgatcgccac gatcgggcgc
     9061 cgcctgcacc ttcggctcct cgacgccggg caagatcttg tagaagtcga agcgcggctt
     9121 gtcgtcggct ttcgcgacct tcgctgcatc gcgcgccggc tcgttcgcgt cgcgcgagaa
     9181 cgtcggcatc ggcagtgccg ggttgttctt catcagccag tacgcgacgc tcgccgccat
     9241 gccgagtccg acgatgatgc cgatgaagac gccgagcagc gtcccgccga gtgcgcctcg
     9301 gggttgcgga gtgcgcgcca tcgaaggccg gcgggatgcg tggatcgagg tcatgggagc
     9361 aaggtgtgcg gcgcggatgg cgtcattgga aacgcgcgcg gaatgcgcgc acaagcgtta
     9421 catggcatcg ggcgcgccga tgccgagcag ggtgaggccg ttgcgcaaca cctggcccac
     9481 ggcgacgatc agcgcaaggc gcgcactgcg tatccggggg tcgtcgacga gaaagcgctc
     9541 cgcattatag taactgtgga attcctgcgc caaatccttg aggtagaacg tgagctggtg
     9601 cggcgcgaat tcgcgcgcgg cgagcgccac ctcgtcggga aaatcggcca ggcggcgcaa
     9661 cagcggcgtc tcgtagcggc tctcgagcga agataggtcc gcgccgcgaa gcgccgcgcg
     9721 cgccgtgtcg aacgggacgc ccgcctgctt caggaccgac gcgacgcgtg catgcgcgta
     9781 ctgcacgtaa tacaccggat tgtcctccga ctgcgagc
//
LOCUS       DASSVX010000320        15474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_880970, whole genome shotgun sequence.
ACCESSION   DASSVX010000320 DASSVX010000000
VERSION     DASSVX010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 15474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15474
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880970"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFO53_13635"
     CDS             <1..355
                     /locus_tag="VFO53_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HET8877368.1"
                     /translation="GAFGALVIGIAAGIGCYFAATTVKGWFRYDDSLDAFGVHCVGGI
                     IGALLTGVLNVRAISGVDGSVLTQAGAVVTTLLVSGVGSYVLLKAIDMTIGLRVTEEQ
                     EREGLDLALHGETVQ"
     gene            complement(434..1750)
                     /locus_tag="VFO53_13640"
     CDS             complement(434..1750)
                     /locus_tag="VFO53_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8877369.1"
                     /translation="MDAGTLGNSAVPAAPPATDERSRRLVTLASLIGTTVEWYDFFIY
                     GTITGLVFNKQFFPSDDVFVSTALAYTVYAVGFVTRPVGGILFGHFGDRIGRKPLLVL
                     TLLIMGISTFLIGLIPNYNAIGIAAPLLLLLLRLLQGIGLGGEWGGAVIMSFEYAPKN
                     RRGFYACFPQVGLAIGLCLATGVVALLSWTLSDAAFQSWGWRVAFLLSIVLVVVGIFV
                     RLRILETPEFTKVREAHKIVPVPIGEVVRDYKKNVLLGWGARWIDGVVFNVYAVFTIA
                     YLAGILKYDKTAILVSISLAAFVLIFTIPVASKWSDRIGRRKVYGWGAFACGVVAFPL
                     MYAMHYSGSALVAGLAIVLALGVIYAPVYGPESALFCELFDTRVRYTGISTVYQVSGI
                     VSSSITPLIATTLLKWNGGEPWYIACYIFVIGMISAVSTYYMRRTF"
     gene            complement(1794..2885)
                     /gene="psrA"
                     /locus_tag="VFO53_13645"
     CDS             complement(1794..2885)
                     /gene="psrA"
                     /locus_tag="VFO53_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase PsrA"
                     /protein_id="HET8877370.1"
                     /translation="MGWTYHNPVAVTFGNGSLDGLRAILGPRRAVVVAFPEAASLGHL
                     DAVRGRAGAAIAGVIDDVEPNPDVVHLRELHARFWREFGSCDAIVALGGGSTLDTAKV
                     LAVTPPAGDFDALVERLAANEAFVPARAKALIAIPTTAGTGSEVTPWATVWDRASGRK
                     HSLHLRETWPEAAIVDPQLTASAPRAITLQSGLDALSHSLEAIWNVNANGVSDALAVS
                     AARRMLRTLPELVERLADATLREQASLAALEAGLAFSNTRTALAHSISYEMTLRHGVP
                     HGIACSFTLPLVLERAIGRRADRDAVLAGVFDVPLARAPEQLSAWLESLGVSTRFETY
                     GVAAGESRAMIAGALDGARGRNFIGAASG"
     gene            2980..3885
                     /locus_tag="VFO53_13650"
     CDS             2980..3885
                     /locus_tag="VFO53_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET8877371.1"
                     /translation="MRLAHLRSFFAVARHRGFTAGARALHVSQPTVTAQVRALEETYA
                     IELFTRRGRNVALTAAGEALYGIAERIFEQEEEALALLQNTGELRAGRLRVGAVGPYH
                     VMEMLAAFHARHPAIDLAVSLGNSEQIVAGLLDRSTDVAVLAQYAHDARLHFVPFRSH
                     RVVLFVPKGHRLARRRGVRLAELASEPMIVREPGSTTRKAMEDALRNAGVAPPIAMEI
                     GSREAVREAVIRGLGIAAVSEIEFVPDPRLQTVPIVDADVETHAHVACLVERRDARVV
                     AAFYDVVHALLATSRRPVRRAATLA"
     gene            complement(3896..4741)
                     /locus_tag="VFO53_13655"
     CDS             complement(3896..4741)
                     /locus_tag="VFO53_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET8877372.1"
                     /translation="MHLAARPLHTRNAYLRDVAQLLALAGDTPVHELTRPMLMRFVAV
                     LHGRGLSGRSLARTLSAWRAFYRFLIERDRAIADDPCAGIKAPKSPKRLPSALSPEEA
                     ARLVAIEGDDLLALRDRALFELAYSSGLRLSELAGLDVAACDLATGEVRVFGKGSKER
                     IVPVGAAAREAITRWLGMRASHAAAGEDAMFVGRHGRRLSARAIEQRLAAWAVRQGLA
                     RHVHPHMLRHSFASHVLQSSGDLRAVQEMLGHASIASTQVYTHLDFQALAKVYDAAHP
                     RAKRK"
     gene            complement(4812..5462)
                     /locus_tag="VFO53_13660"
     CDS             complement(4812..5462)
                     /locus_tag="VFO53_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HET8877373.1"
                     /translation="MDASAVAEYLKQNPAFFEDYADVVAEIFVPHPHGGHAIPIAERQ
                     MLALRDKVYDLEQKLRELIRHGAENDQIGEKLHRSTLALFNASDLETTLAVLSHSLRE
                     DFAVPQVAMRLWPDASLNVSLPELEETATEVREYADRLRAPYCGPEALAAVTGWFGAH
                     SAAQSFAYLPLRTEKTFGLLALASDDARRFHAGIGTVYLLRLAELASVAIARFLRR"
     gene            complement(5482..6312)
                     /gene="dapF"
                     /locus_tag="VFO53_13665"
     CDS             complement(5482..6312)
                     /gene="dapF"
                     /locus_tag="VFO53_13665"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET8877374.1"
                     /translation="MKTLHFTKMEGLGNDFVVVDATRTPFALEAADIRRLADRRFGVG
                     CDQVLVVEPAREGGDFRYRIFNADGGEVEQCGNGARCFVVYVREHGLTGKREIRVETA
                     GGVIVPRLEDDGEVSVDMGVPRFSPRDVPFTGGTGAVVEALDVGGMLVQISALSMGNP
                     HAVLIVDDVESAPVATLGPLIERHPRFPARVNAGFIQIVDRATMRLRVFERGAGETLA
                     CGTGACAAVVAGRRRGLLDEQVRVITRGGALAIRWAGEGSAVVMKGPARTVFEGEWRC
                     "
     gene            complement(6329..6790)
                     /locus_tag="VFO53_13670"
     CDS             complement(6329..6790)
                     /locus_tag="VFO53_13670"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HET8877375.1"
                     /translation="MLVASALLVGACASVALRNPPRIDVVAVALDRVEGPDAYFTVDL
                     ALTNRADEPISIDALDAALSIEGEQVGAAHLVNGPVRLPANGTADAKLTAHSGMDAIL
                     RAVAAAMRRGATLLAPGARPTLRYTLEGGASLQGGGRFPFRKSGELGERKP"
     gene            complement(6844..7938)
                     /gene="ychF"
                     /locus_tag="VFO53_13675"
     CDS             complement(6844..7938)
                     /gene="ychF"
                     /locus_tag="VFO53_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET8877376.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIV
                     EVPDARLAALAAIAKPQKVIPAAVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIAH
                     VVRCFEDPNVVHVAGRIDPIDDIETINTELALADLATVDRQLSKVEKAARGGGDKEAQ
                     RSLAVLTKVRAALNEARPARTADLYDEERALLAPYFLLTMKPTLYVANVAEHGFAGNP
                     MLERLRARAAEEGAPVVAISAALEAQIADLDADDKTLFLDDMGMTEPGLDRLIRAAYE
                     RLGLHTYFTAGPKEVRAWTIPFHATAAQAAGVIHTDFERGFIRAEVIAYADYLAHHGE
                     QGAREAGKMRLEGKDYIVRDGDVMHFRFNV"
     gene            complement(7935..8546)
                     /gene="pth"
                     /locus_tag="VFO53_13680"
     CDS             complement(7935..8546)
                     /gene="pth"
                     /locus_tag="VFO53_13680"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET8877377.1"
                     /translation="MPVPIRLVAGLGNPGRGYAATRHNAGFWFADVLASKLGAAFAHE
                     SKFAADVARSSELRIVKPMTFMNLSGRSVAGIARFFDIAMDAILVVHDELDLLPGEAK
                     LKLGGGVAGHNGLRDIKAQLGSADFWRLRVGIGHPRNSAFPEREVVDYVLKPPDAGER
                     ADIDAAIDRALRAVPDILAGNMERAMMALHTRPPQPPTEAKPE"
     gene            complement(8562..9269)
                     /locus_tag="VFO53_13685"
     CDS             complement(8562..9269)
                     /locus_tag="VFO53_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HET8877378.1"
                     /translation="MATIEFTAFPRSSEGRAASRRMRRTGKAPGIVYGGVTAPQPIEL
                     DHNALIHALRNEAFHASILTMKVDGASTQVLLRDVQMHPFKNEVLHVDFQRIDPARKI
                     HMKVPLHFVNESESPAVTAQGAIVSHVMTEIDIACLPKDLPEYIEVDLSALDTAHSLH
                     VSGVVLPAGVTVVSHRTGDPVVATAVVPKAAAETETVAEGEAAAAAAPAAEAKPAEAK
                     PEEKGGKKEGGKDDRKK"
     gene            complement(9414..9893)
                     /locus_tag="VFO53_13690"
     CDS             complement(9414..9893)
                     /locus_tag="VFO53_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877379.1"
                     /translation="MADATKGEFAALANALRSMHRVLAERVRRDLEAERQSVIQPGAW
                     LSMLVGEPQFAWLRALSELMVDLDVFLEADPQPADDDASAIRAEVERLIAPSAVPGTE
                     SDFARHYWRYVHDEPAVAVVHGEIRSALDHLPLPADVDEAEALHERHAWTEARRHRG"
     gene            complement(9975..10751)
                     /locus_tag="VFO53_13695"
     CDS             complement(9975..10751)
                     /locus_tag="VFO53_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8877380.1"
                     /translation="MPDPIAPDLAGKSVLVTGASTGIGAAAARAFAAAGCRVAIHYNA
                     SLNRAEEVAADVRFAGAEAFLVGGDLRTSAKAREVVDEAASRFGGLDILINNAGGLVR
                     RVPIEEIDDEFFDAVIDLNVRSLVACCSAAVPHMRRGGRGNIVNVTSVAARSGGGNGA
                     AMYASAKGFVSTFTHGLAKELVKLNIRVNAVSPGVIVTPFHERYSTPAMLEASKASIP
                     MNRLGTAEECAGAFLYFASDALSGFVTGQILEVNGGQVMP"
     gene            complement(10783..11733)
                     /locus_tag="VFO53_13700"
     CDS             complement(10783..11733)
                     /locus_tag="VFO53_13700"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET8877381.1"
                     /translation="MAFERMRIFTGNANPRLAEAVCRHLNISLGRAVVGRFSDGEVMV
                     ELLENVRGRDVFVLQSTCAPTNDNLMEVLVMVDALKRASAARVTAAIPYFGYARQDRR
                     PRSARVPITAKVVADMLATVGVDRVMVMDLHADQIQGFFNIPVDNIYATPVLLGDLWK
                     RNYDNLLVVSPDVGGVVRARAIAKRLDSDLAIIDKRRPKANVAEVMNIIGDVNGRTCV
                     IMDDIVDTANTLCKAATALKEHGAQKVVAYCTHPVLSGGAVPRIAASDMDEIVVTDTI
                     PLSADAQACPKIRQLSCAQLLAETMTRISNEESVSSLFTE"
     gene            complement(11810..11886)
                     /locus_tag="VFO53_13705"
     tRNA            complement(11810..11886)
                     /locus_tag="VFO53_13705"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11850..11852),aa:Gln,seq:ttg)
     gene            complement(11931..12770)
                     /gene="ispE"
                     /locus_tag="VFO53_13710"
     CDS             complement(11931..12770)
                     /gene="ispE"
                     /locus_tag="VFO53_13710"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET8877382.1"
                     /translation="MQTLTLAAPAKINLFLHVTGRRADGYHTLESLLVLVDLADSVEL
                     TVRDDGAILRSAPLAGVPERDDLTLRAAHALRDAAQAMRGVAIRVVKRIPQGAGLGGG
                     SSDAATVLLGLNRLWSLQLSRAELMRIGARLGADVPFFLGEGAALARGTGDVLASMSV
                     PACWIALAMPRVHVATASIFASPELTRSAPSAKMDVFSEGYGHNDLEPAAVARFPQVR
                     EAVQALRRASPASRMTGSGACVFAVFASEDDARRALASLPPDVPGCIVRTLNRHPLAS
                     FAA"
     gene            complement(12789..13349)
                     /gene="lolB"
                     /locus_tag="VFO53_13715"
     CDS             complement(12789..13349)
                     /gene="lolB"
                     /locus_tag="VFO53_13715"
                     /inference="COORDINATES: protein motif:HMM:NF015512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HET8877383.1"
                     /translation="MKRALAVIAFALGACASLPPPGGNEAPLAAPDTPFAIAGRISAR
                     RGDAGVAGSFSWRHEPAHDAIELSTPLGQTLARLDGGPTEVTAQLQDGSTQSAPTWRA
                     LTERAFGVTIPIDGLASWIRALPRPGAHFNVERDAAGRVAQLRQDGWDIGYAYADDRA
                     EKPFRVTLSYPGAEPVEVRVVVDRRE"
     gene            complement(13363..15126)
                     /locus_tag="VFO53_13720"
     CDS             complement(13363..15126)
                     /locus_tag="VFO53_13720"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8877384.1"
                     /translation="MTTVRFSLRAAFAAAILTLAASAPAWAAEPADAPASPSAPAAGV
                     ELNADLFYRLLLGDVALQRGDLAVSARAYLDAARSTQDARLAERATEVSIAARDRALV
                     HDAANLWARLDPSAERPKHVLAALESNNNTGAIPSTASNDELRARIEHVLANAALTGP
                     GVGDVFMQINRLFSEQSDKRAVLALVREVAKPYPKTPEAHYAVAMAAFGVGADDAASA
                     REAREEVDRALELRPAWERAAILKAEIAARTSPQDALKVLETFVAANPDAKSAAGALA
                     QRYVDRKRYSDARALMQRLWDRDRDSRDLEFAVATIALEMKDYPEASRLLEDLKSAGY
                     GEPGVIDLYLAQLAEETRQWTKAIEHYQAVTDGDRAWLSKLRIGAMYAKQGQLAKGRQ
                     WLASLTAVTRDQKVQLTQAEAQLLRDAGDDAGAYELLVHGLADNPGAPDLIYDLAMAA
                     EKLDKVDEAESKLKELVALRPDDAQALNALGYTLVDKTSRTGEGLALIQRAHKISPAD
                     PFILDSLGWAFYRMGQFDDAERYLQQALEGRPDAEIAAHLGEVLWRKGEHEKAREVWK
                     AQLVANPDNVLLKETVKRFAP"
     gene            15262..15474
                     /locus_tag="VFO53_13725"
                     /pseudo
     CDS             15262..15474
                     /locus_tag="VFO53_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531709.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
BASE COUNT         2501 a         5295 c         5256 g         2422 t
ORIGIN      
        1 cggcgcgttc ggcgcgctcg tgatcggcat cgccgccggc atcggctgct acttcgccgc
       61 gacgacggtg aagggatggt tcaggtatga cgactcgctc gatgcgttcg gcgtgcactg
      121 cgtcggcggc atcatcgggg cactcctcac cggcgtgctg aacgtgcgcg cgatcagcgg
      181 cgtcgacggc agcgtgctga cgcaggcggg cgcggtcgtg acgacgctgc tcgtaagcgg
      241 cgtcggcagc tacgtcctgc tcaaggccat cgacatgacg atcggcttgc gcgtgaccga
      301 ggagcaggag cgcgaaggcc tggacctggc gctgcacggg gaaacggtgc agtaacgcgc
      361 gacaccctca ccctaccctc tcccgcgagc gggagaggga gcccggcaac agcgaggcgg
      421 cgaagcaggt cgctcagaac gtgcggcgca tgtaatacgt gctgaccgcg ctgatcatgc
      481 cgatgacgaa gatgtagcac gcgatgtacc acggttcgcc gccgttccat ttgagcagcg
      541 tcgtcgcgat cagcggtgtg atcgagctcg agacgatgcc cgacacctgg tacaccgtgg
      601 agatgcccgt gtaacggacc cgcgtgtcga acagctcgca gaagagcgcc gactcgggtc
      661 cgtacaccgg cgcatagatc acgccgagcg ccagcacgat cgcgagcccc gcaacgagcg
      721 cgctgcccga gtaatgcatc gcgtacatca gcggaaacgc gacgacgccg cacgcgaacg
      781 cgccccagcc gtacaccttc cgccgcccga tgcgatccga ccacttcgac gcgaccggaa
      841 tcgtgaagat cagcacgaac gcggcaagcg agatcgacac gaggatcgcg gtcttgtcgt
      901 acttcaggat gcccgccagg taggcgatcg tgaacaccgc gtagacgttg aacacgaccc
      961 cgtcgatcca gcgcgcgccc cagccgagca gcacgttctt cttgtagtcg cggaccactt
     1021 cgccgatcgg cacgggcacg atcttgtgcg cctcgcgcac cttcgtgaac tcgggcgttt
     1081 cgaggatgcg aagccgcacg aagattccga cgaccacgag cacgatcgac agcaggaacg
     1141 cgacacgcca gccccacgac tggaatgcgg cgtcggagag cgtccatgag agcagcgcaa
     1201 cgacacccgt tgcaaggcaa agcccgatcg cgaggcccac ctgcgggaag caggcgtaga
     1261 accctcgacg gtttttcggc gcgtactcga acgacatgat cacggcaccg ccccattcgc
     1321 cgccgagccc gatcccctgc agcaggcgca gcagcagcag cagcagcggc gccgcgatgc
     1381 cgatcgcgtt gtagttcgga atgaggccga tcaggaacgt cgagatcccc atgatcagca
     1441 gcgtcagcac gagcagcggc ttgcggccga tgcggtcgcc gaagtggccg aacaggatgc
     1501 cgccgaccgg acgcgtgacg aagccgaccg cgtacaccgt gtacgcaagc gccgtcgaga
     1561 cgaacacgtc gtcgctcggg aagaactgct tgttgaagac gagtccggtg atcgtgccgt
     1621 agatgaagaa gtcgtaccac tcgacggtgg tgccgatcag gctcgcaagc gtgacgagcc
     1681 ggcggctgcg ctcgtcggtt gcgggcggcg cggccggaac cgcggaattg cctaacgtcc
     1741 ctgcatccat cgttgcctcc ttcagtacgc ggagcgcctc ccgccggctg aggtcagccg
     1801 gatgccgccc cgatgaaatt gcgcccgcgc gctccgtcga gcgcgccggc aatcatggcg
     1861 cgcgactcgc cggccgcgac gccataggtt tcgaagcggg tgctgacgcc gagcgactcg
     1921 agccacgccg agagctgctc cggcgcgcgc gcaagcggaa cgtcgaagac gccggcgagc
     1981 accgcgtcgc gatcggcgcg ccggccgatc gcacgctcga ggacgagcgg cagcgtgaac
     2041 gagcaggcga tgccatgcgg cacgccgtgg cgcagcgtca tctcgtacga aatggaatgc
     2101 gcgagcgccg tgcgcgtgtt cgagaacgcg agtcccgcct cgagcgcggc aagcgacgcc
     2161 tgctcgcgca acgtcgcatc ggcaaggcgc tcgacgagct ccggcagcgt gcgcagcatg
     2221 cgccgcgcag cggaaacggc gagcgcgtcg gagacgccgt tcgcgttgac gttccagatc
     2281 gcttcgagcg aatgcgacag cgcgtcgagg cccgactgca gcgtgatcgc gcgcggcgcg
     2341 ctcgcggtca gctgcggatc gacgatcgcg gcctcgggcc aggtctcgcg cagatgcagc
     2401 gaatgcttgc ggcccgatgc gcgatcccac acggttgccc acggcgtgac ttcgctgccg
     2461 gtaccggcgg tggtcggaat ggcgatcagc gccttcgccc tcgcgggcac gaacgcttca
     2521 ttcgctgcga gccgctcgac gagcgcgtcg aaatcgcccg cgggcggcgt gaccgccagc
     2581 accttggccg tatcgagcgt gctcccgcca ccgagcgcga cgattgcgtc gcacgaaccg
     2641 aactcgcgcc agaaccgcgc gtgcaattcg cgcaggtgaa ccacgtcggg attgggctcg
     2701 acgtcgtcga tgaccccggc aatcgcggcg cccgcgcggc cgcgcaccgc atcgaggtgg
     2761 ccgagcgacg cggcttcggg aaacgcaacg accacggcgc ggcgcggccc gaggatcgcg
     2821 cgaagcccgt cgagcgagcc gttgccgaac gtcaccgcga caggattgtg atacgtccac
     2881 cccatcggac cgcctcgtgg aatgcgagtg gccggagtat cgggatgtgg cgcgcattga
     2941 tccaatacaa aatttgcggc agactcatag aagaaagcta tgcgtctcgc ccacctgcgc
     3001 tcgttctttg ccgtcgcgcg ccaccgcggg ttcaccgccg gcgcccgcgc gctgcacgtg
     3061 agccagccca cggtgactgc acaggtgcgc gcgctcgagg agacctacgc gatcgagctc
     3121 ttcacgcggc gcgggcgcaa tgtcgcgctg accgccgccg gcgaagcgct ctacggcatc
     3181 gccgagcgga tcttcgagca ggaggaggag gcgctcgcgc tattgcagaa caccggcgag
     3241 ctgcgcgccg ggcggttgcg cgtcggtgcg gtgggtccgt accacgtgat ggagatgctc
     3301 gcagcgttcc acgcgcgcca tccggccatc gacctcgccg tgtcgctcgg caattccgag
     3361 cagatcgtcg cgggtctcct cgaccggtcg accgacgtcg cggtcctcgc ccaatacgcg
     3421 cacgatgccc ggctgcattt cgtgccgttc cgctcgcatc gcgtcgtgct gttcgtgccg
     3481 aaggggcatc gcctcgcacg ccggcgtggc gtgcgcctcg ccgagctcgc gagcgagccg
     3541 atgatcgtgc gcgagccggg atcgacgacg cgcaaggcga tggaggacgc attgcgcaat
     3601 gcgggggtgg cgccgccgat cgcgatggag atcggaagtc gcgaggcggt gcgcgaggcg
     3661 gtgatccgcg gcctcggcat cgctgcggtg tcggagatcg agttcgtgcc cgatccacgc
     3721 ctgcaaaccg tgcctatcgt cgatgccgac gtcgaaacgc acgcacacgt cgcgtgcctc
     3781 gtcgagcggc gcgatgcgcg cgtcgtggcc gcgttctacg acgtcgtgca cgcgctgctc
     3841 gcgacgtcgc gacggcccgt ccggcgcgcg gccacgctcg cgtaacgccg cgcgcctatt
     3901 tgcgcttggc gcgcgggtgc gccgcgtcgt acaccttcgc gagtgcctgg aaatcgaggt
     3961 gcgtatagac ctgggtgctg gcgatcgacg cgtgaccgag catttcctgc accgcgcgca
     4021 ggtcacccga cgattgcagt acgtgcgagg cgaacgaatg acgcagcatg tgcggatgca
     4081 cgtgccgcgc caatccctgt cgcacggccc aggccgcgag gcgctgctcg atcgcgcgcg
     4141 ccgacagccg ccggccgtgg cggccgacga acatcgcgtc ctccccggcc gccgcatggc
     4201 tcgcacgcat cccgagccag cgcgtgatcg cttcgcgcgc cgccgcgccc accggcacga
     4261 ttcgctcctt gctgcccttg ccgaacacgc gcacttcacc ggtggcgagg tcgcatgccg
     4321 caacgtcgag tcccgcgagc tcggacaggc gcaatcccga cgagtaggcc agctcgaaga
     4381 gcgcgcggtc gcgaagcgcc agcaggtcgt cgccttcgat ggcaacgagc cgcgcggctt
     4441 cctcgggcga tagcgccgac ggcagccgct tcggcgactt cggcgccttg atccccgcgc
     4501 agggatcgtc ggcgatcgcg cgatcgcgct cgatgagaaa tcgatagaac gcccgccacg
     4561 cggagagcgt gcgcgcaagg ctgcgcccgg acaatccgcg cccgtgcagc accgcgacga
     4621 agcgcatcag catcgggcgg gtgagctcgt gcaccggcgt atcgccggca agtgccagaa
     4681 gctgcgcgac atcgcgcaga taagcgtttc gcgtatgcag cggacgcgcc gcgagatgca
     4741 gcgcgaacgc gtcgacgact gccgcatgcc cggccggaat gactgcgacc ggcttcggcg
     4801 ccctgggcac gctagcgcct caggaagcgc gcgatggcaa cgctcgcaag ttccgcgaga
     4861 cgcagcaggt agacggtgcc gatgcccgcg tggaagcggc gcgcatcgtc gctcgcgagg
     4921 gcgagcaggc cgaacgtctt ttcggtgcga agcggcaggt acgcgaacga ttgcgccgcg
     4981 ctgtgcgcgc cgaaccagcc cgtcaccgcc gcgagcgctt cgggtccgca atacggcgcg
     5041 cgcagccggt cggcatactc gcgaacctcc gtcgccgttt cctcgagctc gggcagcgat
     5101 acgttcaggg aggcgtcggg ccacaggcgc atcgcgacct gcggcaccgc gaagtcctcg
     5161 cgcaggctgt gcgacagcac ggcgagcgtc gtttcgagat cggacgcgtt gaaaagcgcg
     5221 agcgtcgagc ggtgcagctt ctcgccgatc tgatcgtttt cggcgccgtg ccggatgagc
     5281 tcgcgcaatt tctgctcgag gtcgtacacc ttgtcgcgca acgcgagcat ctgccgctcg
     5341 gcgatcggga tcgcgtgccc gccatgcggg tgcggcacga agatttcggc gacgacgtcg
     5401 gcgtaatcct cgaagaacgc cgggttctgc ttcaggtact cggcgaccgc gcttgcgtcc
     5461 atcgacttcc tcttccgttc gttaacaacg ccattcgcct tcgaagaccg ttcgtgccgg
     5521 acccttcatg acgacggcac tcccctcgcc cgcccagcgg atcgcaagcg cgccaccgcg
     5581 cgtgatgacc cgcacctgct cgtccagcaa tccgcgacgc cggcccgcaa cgacggccgc
     5641 gcacgcgccc gttccgcatg cgagcgtttc gcctgcgccg cgctcgaata cgcgcagccg
     5701 catggtagcg cgatcgacaa tctgaatgaa tcccgcattg acgcgcgcgg gaaagcgcgg
     5761 atggcgctcg atcagcggac cgagcgttgc gaccggcgcg ctttccacgt cgtcgacgat
     5821 cagcaccgcg tgcggattgc ccatcgacaa cgcggaaatc tgcacgagca tgccgccaac
     5881 gtcgagcgcc tcgacgacgg cgccggtgcc accggtgaac ggcacgtcgc gcggcgaaaa
     5941 ccgcggcacg cccatgtcga cgctcacctc cccgtcatcc tcgagacggg gcacgatgac
     6001 gccgcccgcc gtctcgacgc ggatctcgcg cttgccggtg agcccgtgct cgcgcacgta
     6061 gacgacgaag cagcgcgcgc cgttgccgca ctgctcgacc tcgccgccgt cggcgttgaa
     6121 gatccggtag cggaagtcgc cgccttcgcg ggcgggctcg acgacgagca cctggtcgca
     6181 gcccacaccg aagcggcgat cggcaaggcg gcggatgtcg gccgcttcga gcgcgaacgg
     6241 cgtgcgggtt gcgtcgacga cgacgaagtc gttgccgagc ccttccatct tggtgaagtg
     6301 cagcgtcttc atccgcgccg cagccgcttc atggcttgcg ctcgccgagc tcgccgctct
     6361 ttctgaatgg gaagcggccg cctccctgca ggctcgcacc gccctcgagc gtgtaacgca
     6421 gcgtcggacg cgcgccgggc gcgaggagtg tcgcaccccg tcgcattgcc gcggcgacgg
     6481 cacgcaggat cgcgtccatt ccgctatgcg ccgtcagttt tgcatcggcg gtcccgttcg
     6541 cgggaagacg caccggacca ttgacgaggt gtgccgcgcc gacctgctcg ccttcgatcg
     6601 acagcgcggc gtcgagcgcg tcgatggaaa tcggctcgtc tgcgcgattg gtgagcgcga
     6661 gatcgacggt gaagtacgcg tcggggcctt cgacgcgatc gagcgcaacg gcgacgacgt
     6721 cgatccgcgg cgggttgcgc agcgcgacgc ttgcgcatgc accgacgagc agcgcggatg
     6781 cgacgagcac ggcatgagcg atccggcttc ctgcccgcgc tcgcccgatc ggccgcacgc
     6841 cgctcacacg ttgaaccgga agtgcatgac gtcgccgtcg cgcacgatgt aatccttgcc
     6901 ttcgagccgc atcttccccg cttccctggc accctgctcg ccgtggtgcg cgaggtaatc
     6961 ggcgtacgcg atcacctcgg cgcgaatgaa cccgcgctcg aaatcggtat ggatgacgcc
     7021 ggcggcctgc gccgcagtcg catgaaaggg aatggtccag gcgcgcactt ccttcggccc
     7081 ggcggtgaag taggtgtgca gcccgaggcg ctcgtaagcc gcgcgaatca gccgatcgag
     7141 ccccggttcg gtcatgccca tgtcgtcgag aaagagcgtc ttgtcgtcgg cgtcgagatc
     7201 ggcgatctgc gcttcgagcg ccgcactgat ggcgacgacc ggcgcaccct cctcggccgc
     7261 gcgagcgcgc agccgctcga gcatcgggtt gccggcgaat ccatgctcgg cgacgttggc
     7321 gacatacagc gtcggcttca tcgtcagcag gaaataaggc gcgagaagtg cgcgctcctc
     7381 gtcgtagagg tcggcggtgc gcgcgggcct cgcttcgttc agcgccgcgc gaaccttcgt
     7441 cagcacggcg aggctgcgtt gcgcctcctt gtcgccgccg ccgcgcgccg ccttctcgac
     7501 cttcgacagc tgccgatcga ccgtggcgag gtccgccagt gcaagctcgg tgttgatcgt
     7561 ctcgatgtca tcgatcggat cgatcctgcc ggcgacgtgg accacgttcg gatcctcgaa
     7621 gcaacgcacg acatgcgcga tggcgtcggt ttcgcggatg ttcgcgagga actggttgcc
     7681 gagcccttcc cccttcgacg cgcccgccac gagacccgcg atgtcgacga attcgacggc
     7741 ggccgggatc accttctgcg gtttcgcgat ggccgcgagt gccgcaaggc gcgcatcggg
     7801 cacttcgacg atgccgacgt tcggctcgat cgtgcagaac ggatagttct cggccgcgat
     7861 gccggccttc gtcagcgcgt tgaagagcgt cgacttgccg acattgggca agccgacgat
     7921 gccgcatttc aggctcattc cggcttcgct tccgtgggcg gttggggcgg ccgcgtgtgc
     7981 agcgccatca tcgcgcgctc catgttgccg gccaggatgt cgggcaccgc ccgcagcgca
     8041 cgatcgatcg ccgcgtcgat gtcggcgcgc tcgccggcat cgggcggctt caggacgtaa
     8101 tcgacgacct cgcgctcggg gaacgcggag ttgcgcggat ggccgatgcc cacgcgcagc
     8161 cgccagaagt cggcgctgcc gagttgcgcc ttgatgtcgc gaagcccgtt atggccggcg
     8221 acgccgccgc cgagcttcag cttcgcctcg cccggcagca ggtcgagctc gtcgtgcacg
     8281 acgaggattg cgtccatcgc gatgtcgaag aagcgcgcga tgcccgcgac ggagcgcccc
     8341 gagaggttca tgaacgtcat cggcttgacg atacggagct cactgctgcg ggcgacatcc
     8401 gccgcgaact tcgattcgtg cgcgaacgcc gcaccgagct tcgacgccag gacatcggcg
     8461 aaccagaacc ccgcgttgtg acgcgtcgcc gcgtagcccc gccccggatt gccgaggccg
     8521 gcgacgaggc gtatcggcac gggcatcgtc tggcggacga attacttctt cctgtcgtcc
     8581 ttgccgcctt ccttcttgcc gcccttctcc tcgggcttcg cttcggcggg cttcgcctcc
     8641 gcagcgggtg cggcggcggc agccgcttcg ccctcggcaa cggtttcggt ttcggcggcc
     8701 gccttcggca cgacggccgt cgcgacgacc gggtcgccgg tgcgatgcga cacgacggtc
     8761 acacccgccg gcaggacgac gccggacacg tgcagcgaat gcgcggtgtc gagggcggag
     8821 aggtcgacct cgatgtattc gggcaggtcc ttcggaaggc acgcgatgtc gatttccgtc
     8881 atcacgtggc tcacgatcgc cccctgcgcc gtcactgccg gcgactcgct ctcgttcacg
     8941 aagtgcaacg ggaccttcat gtggatcttg cgcgccggat cgatgcgctg gaagtcgacg
     9001 tgcagcactt cgttcttgaa cggatgcatc tgcacgtcgc gcagcagcac ctgcgtcgaa
     9061 gcgccgtcga ccttcatcgt caggatcgac gcgtggaatg cctcgttgcg aagcgcgtgg
     9121 atcagtgcat tgtggtcgag ctcgatgggc tgcggcgcgg tgactccgcc gtagacgatg
     9181 cccggagcct ttccggtgcg gcgcatccgg cgcgaggcgg cgcggccttc gctgctgcgt
     9241 gggaacgccg tgaattcgat cgtggccatg gtggcgactc cttcgtggaa agtggtctgc
     9301 ggccttccgg attggctacc ggtcgtccgc gatggtcgcg tgcgcgcggt tcgccgcgcg
     9361 gcgaagaaaa gccttgcagt atagcaaaaa gcgaactggc aaaaggcgaa ccgtcagccg
     9421 cgatggcgcc gtgcttcggt ccaggcatgg cgctcgtgca gcgcctcagc ctcgtcgacg
     9481 tccgccggca ggggcaggtg gtcgagcgcg gagcggattt caccgtggac cacggcgacg
     9541 gccggctcgt catgcacata acgccagtag tgccgcgcga agtcgctttc ggtgccgggc
     9601 acggccgacg gcgcgatcag gcgctcgact tccgcgcgga tcgccgatgc gtcgtcgtcg
     9661 gccggctgcg gatcggcctc gaggaacacg tcgaggtcga ccatcaattc ggacagtgcg
     9721 cgcagccatg cgaattgcgg ctcgccgacg agcatcgaaa gccatgcgcc gggctggatg
     9781 acgctctggc gctccgcttc gaggtcgcga cgcacgcgct ccgcgagcac gcgatgcatc
     9841 gaccgcaacg cattcgcaag tgctgcgaac tcccctttgg ttgcatctgc cacgcttgcc
     9901 gctctccagc tatcccctta ccgccgggtt cgtggcgcct tcctgatcgc tgatatccga
     9961 gccctgatcc ctgatcacgg catgacctgc ccgccgttga cttcgagtat ctgcccggtc
    10021 acgaaccccg acagcgcatc cgacgcgaaa tagaggaatg cacccgcgca ctcctcggcc
    10081 gttcccaggc gattcatcgg aatgctcgcc ttggacgcct cgagcatcgc cggcgtcgag
    10141 tagcgctcgt gaaacggcgt gacgatcacg ccgggcgaga ccgcattcac ccgtatgttg
    10201 agcttgacga gctccttcgc gagtccgtgc gtgaacgtgg acacgaagcc cttcgccgac
    10261 gcatacatgg cggcgccgtt gccgccgccg ctgcgcgcgg cgaccgacgt cacgttgacg
    10321 atgttgccgc gcccgccgcg tcgcatgtgc ggcaccgccg ccgagcagca ggcgacgagc
    10381 gagcggacgt tgagatcgat caccgcgtcg aagaactcgt cgtcgatctc ctcgatcggc
    10441 acgcgacgca cgaggccgcc ggcattgttg atcaggatgt cgaggccgcc gaatcgcgag
    10501 gccgcctcgt cgacgacttc gcgtgccttc gccgacgtgc gcagatcgcc accgacgaga
    10561 aacgcctcgg cgcctgcaaa gcggacgtcg gccgcgactt cctcggcccg gttgagactt
    10621 gcgttgtagt ggatcgcgac gcggcatccg gcggcggcga atgcgcgtgc cgccgcggcg
    10681 ccgatgccgg tcgacgcgcc ggtgacgaga accgacttgc cggcgaggtc gggtgcaatg
    10741 ggatcgggca tggaggatcg tcagcagggg gagtgtttcg tcctactcgg tgaagagcga
    10801 ggacaccgat tcctcgttcg agatgcgcgt catcgtctcc gcaagaagct gcgcgcacga
    10861 cagttgccgg atcttcgggc aggcctgcgc atcggccgac agcggaatcg tgtcggtgac
    10921 gacgatctcg tccatgtcgg aagccgcgat gcgcggaacc gcgccccccg acagcaccgg
    10981 gtgcgtgcag tacgcgacga ccttctgcgc gccgtgttcc ttgagcgccg tcgccgcctt
    11041 gcaaagcgtg ttggccgtgt cgacgatgtc gtccatgatc acgcacgtgc ggccgttgac
    11101 gtcgccgatg atgttcatca cttcggcgac gttcgccttc gggcgccgct tgtcgatgat
    11161 ggcgaggtcg gagtcgaggc gcttcgcgat ggcgcgtgcg cgaacgacgc cgccgacgtc
    11221 gggcgacacg acgagcaggt tgtcgtagtt gcgcttccac aggtcgccga gcagcaccgg
    11281 cgttgcatag atgttgtcga ccgggatgtt gaagaagccc tggatctggt cggcgtgcag
    11341 gtccatcacc atcacccggt ccacgccgac ggttgccagc atgtcagcga ccaccttcgc
    11401 cgtgatcggc acgcgcgccg agcgcggccg ccggtcctgg cgtgcatagc cgaaatacgg
    11461 aatcgcggcc gtgacgcgcg ccgcggacgc gcgcttcagc gcgtcgacca tgacgaggac
    11521 ttccatcagg ttgtcgttcg tcggcgcaca ggtcgactgc agcacgaaca cgtcgcggcc
    11581 gcggacgttt tcgagcagtt cgaccatcac ctcgccgtcg gaaaagcggc cgacgacggc
    11641 acgcccgaga ctgatgttga ggtgccggca gacggcctcg gcgagccggg ggttcgcgtt
    11701 gcccgtgaat atgcgcatgc gctcgaaggc catcggtcgg ctggaaaacg acgggatcag
    11761 cgcgcgcgaa gtggcgtgca cgggacgtgg agttgggcca ggtcaggaat ggctggggag
    11821 gaaggattcg aaccctcgaa tgccggaatc aaaatccggt gccttaacca acttggcgac
    11881 tccccagcgg aaactcacac gcgggcccgc cgtcgcgacg aaccgcccga tcatgctgcg
    11941 aacgacgcca acggatgtcg gttcaacgtt cgcacgatgc aacccggcac gtccggcggc
    12001 aatgatgcca gcgcgcgccg cgcgtcgtct tcgcttgcaa acaccgcgaa gacgcatgcg
    12061 cccgacccgg tcatgcgcga cgccggcgat gctcgccgca atgcctgcac ggcctcacgc
    12121 acctgcggaa acctcgcgac cgcagcaggc tcgagatcgt tgtgtccgta accttcggaa
    12181 aagacgtcca ttttcgccga cggcgccgaa cgtgtcaatt cgggcgacgc gaaaatggac
    12241 gcggtcgcga cgtgcacgcg cggcatcgcg agcgctatcc agcaggcggg cacggacatc
    12301 gatgcaagca cgtcgcccgt gccgcgtgcg agtgccgcgc cttcgccgag gaaaaaggga
    12361 acgtcggcgc ccaatcgagc gccgatgcgc atcaactcgg cgcgcgacag ctgcagcgac
    12421 cacaggcggt tcaacccaag cagcacggtc gcggcatccg agcttccgcc gccgagaccc
    12481 gcgccctggg gaatgcgctt caccacgcga atcgccacgc cgcgcatggc ctgcgcggcg
    12541 tcgcgcagcg catgcgcagc acgaagcgta aggtcgtcgc gctcgggaac gccggccagc
    12601 ggcgcgctgc gcaggatcgc tccatcgtcg cgcacggtga gctcgacgct gtccgcgaga
    12661 tcgaccagga ccagcagcga ttcaagcgtg tggtaaccgt cagcgcgtcg accggtgacg
    12721 tgcaggaaca ggttgatctt cgccggggcg gcgagcgtga gcgtctgcat gtcgcgccga
    12781 aacggccgtc attcgcgccg gtcgacaacc acgcgcacct cgaccggctc ggcgccgggg
    12841 tacgacagcg tcacgcgaaa cggcttttcc gcacgatcgt cggcgtacgc ataaccgatg
    12901 tcccagccat cctggcgcag ttgcgcgaca cggcccgcgg cgtcgcgctc gacgttgaag
    12961 tgcgcgccgg gccgggggag ggcgcgaatc catgacgcca gcccgtcgat cgggatcgtg
    13021 acgccgaagg cgcgttcggt cagcgcgcgc cacgtcggcg cgctttgggt gctcccgtcc
    13081 tggagctgcg cggtcacctc ggtgggaccg ccgtcgagcc tcgccagcgt ctgtccgagc
    13141 ggcgtcgaca gttcgatcgc gtcgtgcgcc ggttcgtgcc gccatgaaaa gctgccggcc
    13201 acgcccgcat cgccgcgacg cgcggaaatg cgtccggcga tcgcgaacgg cgtgtccggt
    13261 gcggcaagcg gtgcctcgtt gccgcccggc ggaggcagcg acgcacacgc gccgagcgcg
    13321 aacgcgatta cggccagcgc gcgcttcatc caagcgcctc gctcacggcg cgaaacgctt
    13381 cacggtctcc ttcagcagca cattgtcggg gttggcgacg agttgcgcct tccatacctc
    13441 gcgcgccttc tcgtgctcgc ccttgcgcca cagcacttcg ccaagatgcg cggcgatctc
    13501 cgcgtcggga cggccctcga gcgcctgttg caggtagcgc tcggcatcgt cgaactggcc
    13561 catccggtag aacgcccagc cgaggctgtc gaggatgaac ggatcggccg gcgagatctt
    13621 gtgcgcgcgc tggatgagcg cgagaccctc gccggtgcgc gacgtcttgt cgacgagcgt
    13681 gtaaccgagc gcgttcagcg cctgcgcatc gtcggggcgc agcgcgacga gctctttcag
    13741 cttcgattcg gcctcgtcga ccttgtcgag cttttcggca gccatcgcga ggtcgtagat
    13801 gaggtcgggc gcccccggat tgtcggcgag gccgtgcacg aggagctcgt atgcgccggc
    13861 atcgtcgccg gcatcgcgca gaagctgcgc ttcggcctgc gtaagctgca ccttctggtc
    13921 gcgcgtcacg gcggtgaggc tcgcaagcca ctggcggccc ttcgcgagct gaccctgctt
    13981 tgcgtacatc gcgccgatcc gcagcttcga gagccaggcg cggtcgccgt cggtgaccgc
    14041 ctggtagtgc tcgatcgcct tcgtccattg ccgcgtctcc tcggcgagct gcgcgaggta
    14101 gagatcgatc acgcccggct cgccgtagcc ggcggacttg aggtcctcga gcaggcgcga
    14161 cgcttcggga taatccttca tctcgagggc gatcgtcgcg acggcgaatt cgagatcgcg
    14221 cgagtcgcgg tcgcgatccc acagccgctg catcagtgca cgtgcgtcgc tgtagcgctt
    14281 gcgatcgacg tagcgctgcg cgagggcgcc cgcggcgctc ttcgcatcgg gattggcggc
    14341 cacgaacgtc tcgagcacct tcagggcgtc ctgcggcgag gtgcgggcgg cgatctccgc
    14401 cttgaggatc gccgcgcgtt cccacgcggg ccgcagttcg agcgcccggt cgacctcctc
    14461 gcgcgcttcc ctggcgcttg ccgcatcgtc ggcgcccacg ccgaatgccg ccattgcgac
    14521 ggcgtaatgc gcttcgggcg tttttggata cggtttcgcc acctcgcgca ccagcgccag
    14581 caccgcgcgc ttgtccgatt gctcggaaaa gagccggttg atctgcatga acacgtcgcc
    14641 cacgcccggg ccggtcaatg ccgcgttcgc gagcacgtgc tcgatgcgcg ctcgaagctc
    14701 gtcgttggag gccgtgctcg gaatggcgcc cgtgttgttg ttcgattcga gcgccgcgag
    14761 cacgtgcttc ggccgttcgg cggacgggtc gagccgcgcc cacaggttgg ctgcatcgtg
    14821 gacgagcgcg cgatcgcgcg ccgcgatcga cacctcggtt gcgcgctcgg cgagccgcgc
    14881 gtcctgcgtc gagcgcgctg cgtcgagata ggcgcgcgcg gaaaccgcga gatcaccgcg
    14941 ctgcaacgca acgtcgccga gcagcaggcg atagaaaaga tcggcgttga gctcgacgcc
    15001 ggcggccggc gcactgggcg aggcgggcgc atcggcgggc tcggccgccc aggccggcgc
    15061 cgacgccgcg agcgtcagga tggcggcggc gaaggcggcg cgcagtgaaa aacggacagt
    15121 cgtcatggga ttccagtgat gcgacaccag gggatcgcgg aagattaagc catagtcgcg
    15181 acgctcacaa attgcccgcg acacgtctcg ccgcgcgcct cacgcgaggc gcccgacggc
    15241 cttcgtcgct accgctcgag gatgcccgaa ctcccggaag tcgaaacgac ccggcgcggt
    15301 attgcgccgc gcgtgaccgg acggcgcgtg gcccggatcg acgtgtacga ctcgcggacg
    15361 cgctggccgg ttcccgcaga cctccgacgc gcgcatgcgc ggacgcacga tcgatcgcgt
    15421 cgaccagcgc agcaaatacc tgctgttcca tgccggcgaa tccgcgctgc tcgt
//
LOCUS       DASSVX010000321         7942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1664100, whole genome shotgun sequence.
ACCESSION   DASSVX010000321 DASSVX010000000
VERSION     DASSVX010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664100"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1009
                     /locus_tag="VFO53_13730"
     CDS             89..1009
                     /locus_tag="VFO53_13730"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET8877385.1"
                     /translation="MTDARPTGVYPIAPTPFDDDGGLDLDGQRRVLDCMIDQSVDGIC
                     ILANYSEQFLLSDAERDTLLALCLAHVAGRVPVIVTCSHFSTRVAIERARAAAAAGAS
                     MLMLMPPYHGTALRADDNGVVEHYARIAGAVDLPIMVQDAPLSGVNLPVPLLVRLCHE
                     VPSVRHFKIEVPGAAAKLRSLIEAGGDAIAGPFDGEESITLMADLEAGARGTMPSALL
                     PDLIRPVVVDFLAGRTSDAADAYARILPLVNYENRQCGLRATKTVMMEGGVIRSDAVR
                     HPLAPLHPATRRGLLALAAEAAPLALRWGR"
     gene            complement(1071..2423)
                     /locus_tag="VFO53_13735"
     CDS             complement(1071..2423)
                     /locus_tag="VFO53_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010121107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET8877386.1"
                     /translation="MSSEGQPGEGTMEAYFSALAAGVDRALGAGESYTLDFAAEATDF
                     VRMNRGKVRQPGHVEQCYVDLRLIRGARHASHLLSLTGDVAADARIVDAALAGLRDAL
                     PELHDDPHLLLPDVVASSRSECCETMPAPEAMIDALLTMGRGQDLVGMLAAGPVYRGF
                     ANAQGQRNWHATTTFNLQWSLYHRADKAVKSSYTGFAWRDAELQAKMAEAIEQLALIS
                     RPPKALAPGKYRAYLPPAALQEVTDLLAWGGFSARALATQQSPLAKMRANARLDPRVS
                     MSEDIASGVAPAFQAEGFARPGAVPLIVAGELAGSLVSPRTAREFDLLTNGANGGESP
                     EALSLAGGELAAGDALGALDTGLAIGNLWYLNFSDRPACRMTGMTRFATFWVENGKIV
                     APVNVLRFDDTIYRMLGDNLEALTAERELLLEANSYGSRMLSSVRLPGVLLSGMNFTL
                     "
     gene            complement(2420..3847)
                     /locus_tag="VFO53_13740"
     CDS             complement(2420..3847)
                     /locus_tag="VFO53_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007734125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET8877387.1"
                     /translation="MDNLRLPVLAIEADYWSLRFVEETCQSYAVRKNIPQPLSVITDH
                     GVMASVYADGGYGYAASPDTSEAGLRNVLERAARWARASARYALDDSRTLPRPAPRGD
                     YASPSIDAPLPSRREWYELLRHESEAAGCDPRIVDWEALVDVRNATHRLVTSEGGDVV
                     QRYRFLFPSLSVTAHADGVTQQRSLNGYRGICQQGGVEILARFGFAGAGRRVAEEALE
                     LLAAPNCPSERMDVLLAPDQMVLQIHESIGHPLELDRILGDERNFAGTSFVTPEMFGT
                     YQYGSERLNVTFDPTRPEELASYAFDDEGTRAERTYLIRDGVLVRPLGGAISQKRAGL
                     PGVANARADGWNRPPIDRMANLNLEPGDATLDELVAGIERGVMMETNSSWSIDDSRNK
                     FQFGCERARLIENGALGAVVKNPNYRGISAQFWRSLARVGNADTIRVLGTPYCGKGEP
                     GQTIRVGHASPACVFTNVDVFGGES"
     gene            complement(3902..4423)
                     /locus_tag="VFO53_13745"
     CDS             complement(3902..4423)
                     /locus_tag="VFO53_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010116380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8877388.1"
                     /translation="MHASAGDVADLHTLVRALAEYERLAHLCTSSEGDLAAALFGPHP
                     VAEAMIARLDAESQDCIGFALFFHTYSTFLGRRGLWLEDLFVKPAHRGAGVGRALLAR
                     LAVLARERGCGRFEWSVLDWNRSAIAFYEGIGATVLSDWRIARVTGDALAQLASEAAP
                     QAPAGEAGLADRR"
     gene            complement(4473..4967)
                     /locus_tag="VFO53_13750"
     CDS             complement(4473..4967)
                     /locus_tag="VFO53_13750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8877389.1"
                     /translation="MPIRHAAPEDLPEIVAIYNASIPGRMATADLAPIGVADRMQWFA
                     EFDPRRRPIFVSCADDDPAPRAWLSLRSFYGRPAYDETVEVGLYTAPTAQRRGHGRRL
                     VEHALREAPSLGITTLLAFTFAHNAPSLALFRKAGFGDWGRLPRVAKLDGVRRDVIIL
                     GRAL"
     gene            5119..7881
                     /locus_tag="VFO53_13755"
     CDS             5119..7881
                     /locus_tag="VFO53_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET8877390.1"
                     /translation="MNFQRKKVAAALACIAGVGGAMAMSGALAQAVNPDVPSNTRPEA
                     RPDIRVEVTGSNIKRVEGEGALPVTVITREQIDKSGATTPMELLQQISANNSVGAVSI
                     ANSVGATTLSAQTASLRGLGGGRTLVLINGHRIEGFPGEIQGVQGVTLSSIPFNAIER
                     VEILKDGASAVYGSDAIAGVINFITRSDYTGAEVTGYYGSPTRGGGGDQWQGSASAGF
                     GDLSKDRYNVFLSGQYNEQRPLDQAARDFSNSSYRPEIGLIGLSSNTNPGNIRTRGTD
                     GSRIGVVNNSSGTAVIANSAATCAPGIFVNDDILGTGCFFDPSAARGVEMIPEDKSYS
                     FFGGGKFQINNDWQLYAQGLYTHEETHLIIQPGPISSAISYGPGGDLAATITLQPTSP
                     FYPHNIAAAAGIDGTPLDVRYRTFDNGFRDTTDTNEHWQGIFGVKGSWMNWDWDGSVF
                     YAEGTTTEHLNSGFQDYRLLLPLLNSGTVNLFGPNTPDIVNQLRATNFVGDTIDGKSK
                     NYGAQIKTSGEIWKLPAGPLAMAVGLEARKEELVQNMAEGIQSGFITGYGGEIKNVEG
                     SRKQWAAFTEFNIPVVKTLEADVAVRYDHYSDFGSTTNPKFSLRWQPTRTLLVRGSYG
                     TGFLAPSLYQLFTPNIAGLSQPGLTDPVRCPITNDQTFDCNAQFNVLFGGNRNLKPET
                     SEQTTAGVVFEPIPNVSMSVDWFKINLTNAITNGISPITILGDLGQFGGLVTRDATGH
                     IASILQTYINLGTVKIEGIDLEGHYRAPTMSWGRISLDGSGTYYTKYDNQQNDGSYVS
                     QIGTAYQQVVTGITPRWKQYVSATWDLGPWSATIANTYQTSYIDQQTDLDGNLRKVGS
                     LSLWDLQGSYTGFKNLKLTLGVKNLFDRNPPVSNQQSTFIVGFDPSYYDPRARFVYGQ
                     VNYRFK"
BASE COUNT         1437 a         2645 c         2605 g         1255 t
ORIGIN      
        1 tagattcgca cgttcgaacc atcaccggag gcgaaaccat gaagcgctgc cgacgcgaac
       61 ccttgcacga ccacccggcg cacgcatcat gacggatgcg cggccgacgg gcgtctatcc
      121 gattgcgccc acgccgttcg acgacgacgg cggcctcgac ctcgacggcc agcggcgagt
      181 gctcgattgc atgatcgacc aaagcgtcga cggcatctgc atcctcgcca attactcgga
      241 gcagttcctg ctttcggatg ccgagcgcga cacgctgctt gcgctgtgcc tcgcgcacgt
      301 cgccggccgt gtgccggtca tcgtcacgtg cagccatttc agcacgcgcg tcgcgatcga
      361 acgcgcgcgt gcggccgcgg cggccggagc gtcgatgctg atgctgatgc cgccgtatca
      421 cggcacggca ttgcgcgccg acgacaacgg cgtcgtcgag cattacgcgc gcatcgccgg
      481 ggccgtcgac ctgccgatca tggtgcagga cgcgccgctg tccggcgtga acctgcccgt
      541 gccgctgctc gtgcgcctgt gccatgaggt gccatcggtt cgtcatttca agatcgaagt
      601 gcccggcgcg gccgcgaagc tgcgcagcct gatcgaggcg gggggcgacg cgatcgcggg
      661 acccttcgac ggcgaggaat cgatcacgct gatggccgat ctcgaggcgg gcgcacgggg
      721 cacgatgccg agcgcattgc tccccgacct catccgtccg gtcgtcgtcg acttccttgc
      781 cggtcgcacc agcgacgcgg ccgacgcgta tgcgcgcatc ctgccgctcg tgaactacga
      841 aaatcgacaa tgcggactgc gcgcgacgaa gacggtgatg atggaaggcg gcgtcatcag
      901 gtcggacgcc gtgcgccatc cgctggcgcc gttgcatccg gcgacccgcc gcgggttgct
      961 cgcgctggcc gccgaagcgg cgccactcgc gttgcgctgg ggccgctgaa ttggggtcag
     1021 agctgtaatt attcgttccg cggaataatt acagctctga ccccaattgt ttacagcgtg
     1081 aagttcatcc cggacagcag cacgccgggc agccgcacac tcgaaagcat gcgcgagccg
     1141 tagctgttcg cttcgagcag cagctcgcgc tcggcggtaa gcgcctcgag attgtcgccg
     1201 agcatgcggt agatcgtgtc gtcgaagcgc agcacgttca ccggtgccac gatctttccg
     1261 ttttcgaccc agaacgtcgc gaagcgcgtc atccccgtca tccggcacgc cggacgatcg
     1321 gagaagttga ggtaccacag gttgccgatg gcgaggccgg tatcgagtgc gccgagcgca
     1381 tcgccggccg cgagctcgcc gcccgccagc gaaagcgcct ccggcgattc gccgccgttc
     1441 gcgccgttgg tgagtagatc gaactcgcgc gcggtgcggg gcgacacgag cgagccggca
     1501 agttcgccgg cgacgatcag cggaacggcg ccgggccgcg caaagccctc ggcctggaat
     1561 gcgggagcga caccggatgc gatgtcctcg ctcatcgaca cgcgcgggtc gagccgcgcg
     1621 ttcgcgcgca ttttcgcgag cggactttgc tgcgttgcga gcgcgcgtgc cgagaatcca
     1681 ccccacgcga gcaggtcggt gacttcctgc agcgcggcgg gcggcagata cgcgcggtat
     1741 tttcccggcg cgagcgcttt cggcgggcgg ctgatcagcg cgagctgctc gatcgcttcg
     1801 gccatcttcg cctgcagctc ggcatcgcgc catgcaaagc ccgtatacga cgacttgacc
     1861 gccttgtcgg cgcggtgata aaggctccat tgcaggttga acgtcgtcgt cgcatgccag
     1921 ttgcgctggc cttgcgcgtt cgcgaagccg cgataaacgg gaccggcggc gagcatgccg
     1981 acgaggtcct gcccgcggcc catcgtcagc agcgcatcga tcatcgcttc gggtgcgggc
     2041 atcgtctcgc agcactcgct gcgcgacgac gcgacgacat cgggcagcaa cagatgcgga
     2101 tcgtcgtgca actcgggcag cgcgtcgcgc aatccggcga gcgccgcgtc gacgatgcgc
     2161 gcatcggccg cgacatcgcc ggtcagcgac aagaggtgcg acgcatgacg cgcgccgcga
     2221 atcaggcgca ggtcgacgta gcactgctcg acgtgccccg gctggcgcac cttgccgcga
     2281 ttcatgcgca cgaagtcggt cgcctccgcg gcaaagtcga gcgtgtacga ctcgccggcc
     2341 ccgagcgcgc gatcgacgcc cgcagcaagc gcgctgaagt acgcttccat cgtgccctcg
     2401 ccaggctgtc cctcggagct cacgattcgc ccccgaacac gtcgacgttc gtgaacacgc
     2461 acgcgggcga cgcgtggccg acgcgaatcg tctgccccgg ctcgcccttg ccgcagtacg
     2521 gcgtgccgag cacgcgaatc gtgtcggcgt tgccgacccg cgcgaggctg cgccagaact
     2581 gggcgctgat cccgcggtag ttcggattct tcaccaccgc gccgagcgcg ccgttctcga
     2641 tcaatcgcgc gcgttcacag ccgaactgga acttgttgcg cgaatcgtcg atcgaccacg
     2701 acgagttcgt ctccatcatg acgccgcgct cgatgcccgc gacgagctcg tcgagcgtcg
     2761 catcgcccgg ctcgagattg agattggcca tgcggtcgat cggcgggcgg ttccagccgt
     2821 cggcgcgcgc gttcgccacg cccggcaggc cggcccgctt ctgcgagatc gcaccgccga
     2881 gcggacgcac gaggacacca tcacggatca gatacgttcg ctcggcgcgc gtgccttcgt
     2941 cgtcgaacgc atagcttgcg agctcctcgg gccgcgtcgg atcgaacgtg acgttgaggc
     3001 gctcggaccc gtattgatag gtgccgaaca tttccggcgt cacgaagctc gtgccggcga
     3061 aattgcgctc gtcgccgagg atgcggtcga gctcgagcgg atggccgatc gactcgtgga
     3121 tctgcagcac catctgatcg ggcgcgagca gcacgtccat gcgctccgag gggcaattcg
     3181 gcgcggcgag cagctcgagc gcttcctccg cgacgcggcg gccggcgcct gcgaagccga
     3241 accgcgcgag gatctcgacg ccaccctgct gacagatgcc gcgatagccg ttaagcgacc
     3301 gctgctgcgt cacgccgtcg gcgtgcgcgg tgacgctgag cgacgggaac aggaagcggt
     3361 aacgctgcac gacgtcgccg ccctcgctcg tcaccagccg gtgcgtcgca ttgcgcacgt
     3421 cgacgagcgc ttcccaatcg acgatgcgcg gatcgcagcc ggctgcttcg gattcgtgtc
     3481 gcagcagctc gtaccactcg cgccgcgacg gcaacggggc gtcgatcgac ggcgacgcgt
     3541 agtcgccgcg gggcgccggg cggggcagcg tgcgcgaatc gtcgagcgcg tagcgggcac
     3601 tggcgcgtgc ccagcgtgcg gcgcgctcga ggacattgcg aagccccgcc tcggaggtgt
     3661 ccgggctagc cgcatacccg tagccaccgt cggcgtagac ggaagccatg acgccgtgat
     3721 cggtgataac cgagagcggc tgcggaatgt tcttgcgaac ggcatacgac tggcaggtct
     3781 cctcgacgaa gcgcagcgac cagtagtcgg cttcgatcgc gaggaccggg agacgaaggt
     3841 tatccatggg gtgaccgcga aggcgcgagg cgcgcccgtg ggccgggacc gaaagacgcg
     3901 attatcgccg atcggccaat ccggcttcgc cggccggtgc ttgcggggct gcctccgagg
     3961 caagctgcgc gagcgcgtcg ccggtgacgc gggcgatccg ccagtcggac aggaccgtgg
     4021 cgccgatccc ttcgtagaac gcgatggccg accggttcca gtcgaggacc gaccactcga
     4081 agcggccgca gccgcgctcg cgcgcgagga cggcaaggcg cgcaagcagc gcccgtccaa
     4141 cgccggcgcc acggtgggcg ggcttgacga aaagatcctc cagccataga ccccgccggc
     4201 ccaggaaagt cgagtaagta tgaaaaaaca acgcgaatcc gatgcaatcc tgagattccg
     4261 cgtcaagacg cgcaatcatc gcctcggcga ccgggtgcgg gccgaacaac gcggcggcga
     4321 gatcgccctc gctcgacgtg caaaggtggg cgaggcgctc gtattcggcg agcgcccgca
     4381 ccagcgtgtg cagatcggca acgtcgccgg cgctggcgtg catgatccgg aacgaacgaa
     4441 cgttcgacat cagcggcgcg gctgttgcgc cgtcacaacg ccctcccgag gatgatcacg
     4501 tcgcggcgaa cgccgtcgag cttcgccacg cgcggcagtc ttccccagtc gccgaacccg
     4561 gccttgcgaa acagcgcgag gctcggcgca ttgtgcgcga acgtgaaggc aaggagcgtg
     4621 gtgatgccga gcgaaggcgc ctcgcgcagc gcgtgctcga cgagccgccg cccatgcccg
     4681 cggcgctgcg cagtgggggc ggtgtaaaga cccacttcca ccgtttcgtc ataagccggg
     4741 cgcccgtaga acgagcgcag cgacagccag gcccggggcg ccgggtcatc gtcggcgcac
     4801 gacacgaaga tcggccgtcg gcgcggatcg aactcggcaa accactgcat gcggtcggcg
     4861 acgccgatcg gggcgagatc ggccgtcgcc atccgcccgg ggatcgacgc gttgtagatc
     4921 gcgacgattt ccgggaggtc ttccggcgcg gcatggcgaa tgggcatttg acgaaattgc
     4981 aacaaaagtg ccgctcaggg gcaaggaatc ggggagtttg attgccgctt caaacggcgc
     5041 tgttgtaacg ttgctactgc gtgccgaaag gcgaatattt cgtcccttcc agtaccactt
     5101 tcctttgggg gtttccacat gaatttccaa cgcaagaagg tcgcggccgc gctcgcctgc
     5161 atcgcaggcg tcggcggcgc gatggcgatg tccggggcac tcgcgcaagc ggtcaatccg
     5221 gacgtgccat cgaacacgag acccgaggcc cggcccgaca tccgcgtcga ggtcacgggt
     5281 tcgaacatca agcgcgtcga gggcgaaggc gcattgccgg tcacggtgat cacgcgcgag
     5341 cagatcgaca agtcgggcgc caccacgccg atggaactgc tgcagcagat ttcggcaaat
     5401 aacagcgttg gcgcagtcag catcgcaaat tccgtcggtg caacgacgct ttccgcgcaa
     5461 accgcgtcgc tgcgcggtct gggcggcggc cgcacgctgg tgctgatcaa cgggcatcgg
     5521 atcgaaggct tcccgggcga aatccagggg gtgcagggcg tcacgctttc gtcgattccc
     5581 ttcaacgcga tcgagcgcgt ggaaatcctg aaggacggcg catcggccgt gtacggttcc
     5641 gacgccatcg cgggcgtcat caacttcatc acgcggtcgg attacaccgg cgcggaagtc
     5701 accggctact acggctcgcc gacgcgcggc ggcggcggcg accaatggca gggtagcgct
     5761 tcggccggtt tcggcgatct cagcaaggat cgctacaacg tcttcctttc cggccagtac
     5821 aacgagcaaa ggccgctcga ccaggccgcc cgcgactttt cgaacagctc ctatcggccg
     5881 gaaatcggac tcattggtct gtcgagcaac acgaatccgg gcaacatccg aaccagggga
     5941 accgacggct cgcgcatcgg ggtcgtcaac aatagcagcg gtacggcggt catcgccaac
     6001 agtgcggcaa cctgcgcgcc gggcatcttc gtcaacgacg acatcctcgg aaccggatgc
     6061 ttcttcgatc cgtcggccgc gcgcggcgtg gaaatgatcc ccgaggacaa gtcgtacagc
     6121 tttttcggcg gcggcaaatt ccagatcaac aacgactggc aactgtacgc gcaggggctg
     6181 tacacgcacg aagaaacgca cctcatcatc cagccggggc cgatctccag tgccatttcc
     6241 tacgggccgg gcggcgatct cgccgcgacg attacgctgc agccgacgag cccgttctac
     6301 ccgcataaca tcgccgcggc ggccggcatc gacggcacgc cgctcgacgt gcgctatcgc
     6361 acgttcgaca acggcttccg cgacaccacc gacaccaacg agcactggca gggcatcttc
     6421 ggcgtcaagg gcagctggat gaattgggac tgggacggct cggtcttcta cgccgaaggc
     6481 accacgaccg agcacctgaa cagcggcttc caggattacc ggctgctgtt gccgctgctc
     6541 aacagcggga cggtcaacct gttcgggccg aatacccccg acatcgtcaa ccagctccgc
     6601 gcgaccaatt tcgtcggcga tacgatcgac ggcaagtcga agaattacgg cgcgcagatc
     6661 aagacctccg gcgaaatctg gaagctgccg gccggtccgc tcgcgatggc ggtgggtctc
     6721 gaggcgcgca aggaagagct ggtccagaac atggccgaag ggattcagtc gggcttcatc
     6781 accggctacg gcggcgagat caagaacgtc gaagggtcgc gcaagcaatg ggcggcgttc
     6841 accgagttca acatcccggt cgtgaaaacc ctggaagccg acgtcgctgt tcgctacgac
     6901 cactacagcg atttcggcag caccaccaat ccgaagttca gcctgcgctg gcagccgacg
     6961 cgcacgttgc tggtgcgcgg ctcctacggt acgggcttcc tcgcgccgag cctctaccag
     7021 ctcttcacgc cgaacatcgc gggcctcagc cagccgggtc ttaccgaccc ggtccgctgt
     7081 ccgatcacca acgaccagac gttcgactgc aatgcgcagt tcaacgtgct gttcggcggc
     7141 aaccggaatc tcaagcccga gacgtccgag cagacgaccg ccggcgtggt gttcgagccg
     7201 atccccaatg tctcgatgag tgtcgactgg ttcaagatca acctgacgaa cgcgatcacg
     7261 aacggcattt cgccgatcac gatcctcggc gatctcggcc agttcggcgg cctcgtcacg
     7321 cgcgatgcga ccggtcacat cgcgtcgatc ctgcagacgt acatcaacct cggcacggtg
     7381 aagatcgaag gcatcgatct cgaagggcat taccgcgcgc cgacgatgag ctggggccgc
     7441 atctcgctgg acggctcggg cacctactac acgaagtacg acaaccagca gaacgacggc
     7501 tcgtacgtga gccagatcgg caccgcgtac cagcaggtgg tcacgggcat cacgccgcgc
     7561 tggaaacagt acgtatcggc aacgtgggat ctcggtccgt ggtcggcgac gatcgccaac
     7621 acgtaccaga cgtcgtacat cgaccagcag accgacctcg acggcaacct gcgcaaggtg
     7681 ggctcgctgt cgctgtggga cctgcagggc tcgtacacgg gcttcaagaa cctgaagctc
     7741 acgcttggcg tgaagaacct gttcgatcgt aatccgccgg tgtcgaacca gcagagcacg
     7801 ttcatcgtgg gcttcgatcc gtcgtactac gacccgcgcg cccgcttcgt ctacggccag
     7861 gtcaactatc ggttcaaata agcgacgaag aacggcagta ccgcagttga ttcgcggggg
     7921 gcttcggccc cccgtttttt cg
//
LOCUS       DASSVX010000322        11360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_281536, whole genome shotgun sequence.
ACCESSION   DASSVX010000322 DASSVX010000000
VERSION     DASSVX010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 11360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11360
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281536"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="VFO53_13760"
     CDS             complement(<1..1219)
                     /locus_tag="VFO53_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HET8877391.1"
                     /translation="MATLSAVPAGPRLWVIGDLNGFFGLFTNVLLNVIVLTGLCLGVV
                     QLPADTVFGRILPALGIALPIGNLFYAYLAWQLGKQEGRTDVTALPYGPSVPHMFIVV
                     FLIMLPTYLATKDPLVAWRAGLAWAFIIGVIVLIGAFVGPKIRSLTPRAAMLGTLAGI
                     SIAFISMRPAFQMWEVPWIGFVCFALVLIAWTANVRLPGGVPGGLAAVIVGSVIGWVV
                     SLVGWSDYMQPAAVAKSFEQFGLRVPLLSADVWQGLAQVAPLLVTAIPLGIYNFTEGM
                     NNVESASAAGDNYNLRKVLLADGIGAIVGSVLGSPFPPAVYIGHPGWKAVGGRIGYSL
                     ATGIAIAIVCFLGLTAFLLAVIPLVAILPILLYIGLVIGAQAFQVTPKSHAPAVVLAL
                     IPNIAEWAKTQIDG"
     gene            complement(1235..1633)
                     /locus_tag="VFO53_13765"
     CDS             complement(1235..1633)
                     /locus_tag="VFO53_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017288127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HET8877392.1"
                     /translation="MTNDVLFAVNGTLMRGLALNANLVTAGATFVRDDETDACYRIWS
                     IDDRHPAMLRTPGEGTNVAVEVWQVPVAGLVSILLAEPPGLAVGKVVLRDGSIVLGVL
                     GEPFLCEGKREITEYGGWRAYMASKGRHTT"
     gene            complement(1650..2597)
                     /gene="arcC"
                     /locus_tag="VFO53_13770"
     CDS             complement(1650..2597)
                     /gene="arcC"
                     /locus_tag="VFO53_13770"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006229932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET8877393.1"
                     /translation="MTDRIAVVAVGGNALIRDDAHTALNDQYDAVCATAAHIVDMVVA
                     GWNIVVTHGNGPQVGFILRRSEIADGQVPTVPIRYAVGDTQGAIGFMFQNALGNALRR
                     RRIDKPVVTLVTQTLVDHDDPAFTHPDKPVGSFLTEDIARGLERELGWSIMRDGDRGW
                     RRCVASPQPLRIVEAPIVARLARDGVLVIACGGGGIAVEQTAGGELQGVEAVVDKDRA
                     SALLAIELSADLLMIPTGVERVAIRFGAPDQQWLDRIGIEQAERFAAQGHFGEGSMRP
                     KIEALLTFVRKRHGGRGVITNPENIARALRGETGTWIES"
     gene            complement(2629..4092)
                     /locus_tag="VFO53_13775"
     CDS             complement(2629..4092)
                     /locus_tag="VFO53_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HET8877394.1"
                     /translation="MKALFKDTLKVVNVGLAGFGDNVTAAGGACIATAWQPPALGDRD
                     AGWALAQIFDDAVVDHANAVAFARFLEANPVLTDVAVARDVIAAMGSGRRLILHAGPP
                     IAWAAMCGPMRGAVLGAVLLEGWADTIDAATRLVDAGKIALEPCHHHGAVGPMAGIIS
                     PSMPVWVVENATGGNRAYSNFNEGLGKVLRFGAHDRSVLDRLRFIASDVAPALRAAVR
                     QLGRIELKPLMAQALNMGDEVHNRNAAATSLLFKQLTPAVLTSDIPRERALAALNFIA
                     GNDHFFLNLSMAACKAMLDAAAGVPASSMVTAMSRNGVNFGIRLSGTGERWFEAPANP
                     VDGLYFPGYSRADAAADLGDSSITETAGVGGFAMAAAPAIVKFVGGTAADALHHSLRM
                     RAITLGANPSFTLPALDFAAVAAGIDARKVVDRGILPVINSGIAHREPGVGQIGAGIT
                     SAPMACFSAAVKALAGDLRAIPSRQPSPRTRGEGAER"
     gene            complement(4089..5669)
                     /gene="fdrA"
                     /locus_tag="VFO53_13780"
     CDS             complement(4089..5669)
                     /gene="fdrA"
                     /locus_tag="VFO53_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HET8877395.1"
                     /translation="MTTEIRFRVFTNLYKDSVALMQVGAALRQRPGIVEASCIMATPA
                     NLAQLQHADLSVDADAHPSDLLVVVRGEAQACDDAIDAAQALLQGAPREADQRDDAYS
                     MPLTSIAAAIEQTPDADLALISVPGDYAAAEAMKALSLGLHVMLFSDNVGVDQEVAVK
                     RHAEERGLLVMGPDCGTAIINGIPLGFANVVRRGDIGLVAASGTGLQEVTCRIHNRGA
                     GVSQAIGTGGRDLRDEIGGLTMLAALKALADDDRTRVIVLISKPPSPTVASRILALAA
                     GSGKPTVVHFLGAAPGTVQGARLHAAQSLADAADIAVALSRGSERASASAIAANDASA
                     DRVHVVDGVANTQRTVRGLFTGGTFCYEAQLAFLHRGLACRSNAPVHGALPLDGSTAD
                     HVFIDMGDDEYTRGRPHPMIDPSLRNAAIREAADDPAVALLLFDLVLGFGSHPSPADE
                     LADALRDAQRAAALGGRKLVPIGHVCGTDGDPQDRAVQIRTLASAGAIVADSNIEAAS
                     LAAQLALRLAQRHAARMP"
     gene            complement(5912..6700)
                     /locus_tag="VFO53_13785"
     CDS             complement(5912..6700)
                     /locus_tag="VFO53_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8877396.1"
                     /translation="MTADYIVQLEDISKFFGSVIALNGVTLRLRRGEVHCLLGDNGAG
                     KSTLIKTLAGVHPPDRGEYLVDGRRMRFKSPREALDLGIATVYQDLALVPLMSVARNF
                     VMGREPRRRLFGPFQVMDHAQAGRTAIEKLAEMGIRMRDASQVVGTMSGGERQCLAIA
                     RAIYFGARVLILDEPTAALGVRQSVNVLKLIHRARTKGISVIFITHNVNHAYPVADTF
                     TLLNRGKSLGTFAKRDVTKAQVLDMMAGGAEMQALMADLEGVTM"
     gene            complement(6815..7972)
                     /locus_tag="VFO53_13790"
     CDS             complement(6815..7972)
                     /locus_tag="VFO53_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8877397.1"
                     /translation="MAGPDTSSPVIRATHAPSSDERVRDIGALRRFLARPEFGSISGA
                     VLVFVLFGLVAGDSGMFSAEGIVNWATVAAFLGILAVGAALLMIGGEFDLSIGSMIGF
                     AGMMLAIPALYWGWPLWLAAVFTFALCAALGMLNGCLVVRTGLPSFIVTLAFYFILRG
                     LSLALSTMLTGKTIISSNEGVALRDLATRDPLFSTLFSGHVLTGLFDTMARWGWIAAK
                     DNGSALAIGVPKVIVWWLLLTAIGGFVLARTRRGNWILAVGGDANAAKNTGVPVARVK
                     VGLFVVTALCSGLFAVLQVADAGSAAADRGLQKEFEAIIAAVIGGCLLSGGYGSVVGA
                     SIGALIFGVVQIGIGYTSIDNNWYRVFLGGMLLAAVVFNTYVRTRVALGRR"
     gene            complement(7993..8961)
                     /locus_tag="VFO53_13795"
     CDS             complement(7993..8961)
                     /locus_tag="VFO53_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET8877398.1"
                     /translation="MGTAYGAGRYVLISHAPDSDSWWNVIKNSIKQAGEDYDVTVDYR
                     NPPSGDLADMARLIEQAAAADYDGVITTIADYNVLQGAIGKVTAKKIPLITINSGTTE
                     QSEKLGAIMHVGQPEYAAGKGAGERAKAAGVKSFLCVNHYATNPASFERCRGFAEAIG
                     ADYKKSTLDVGDDPTTIESKVSAYLRQHPETNGVLALGPTSASPTIRALEKSGKAGKL
                     WMATFDLSDDISKAIKSGTVKFAIDQQPYLQGYIPVAVLATMKQMKTTDLKKVADAVK
                     ADPKTKKRFDEYGLVPNYGPRHISSGPGFITKDNIAKVEKYAGQYR"
     gene            9219..11354
                     /locus_tag="VFO53_13800"
     CDS             9219..11354
                     /locus_tag="VFO53_13800"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HET8877399.1"
                     /translation="MLAYRPPAVAKLPATTTHVPDYLASLNAQQRLAVEHGVGPTAND
                     RGPLLIIAGAGSGKTSTLACRAAHLIANGADPQRILLLTFSRRAAEALERRAGQFLHR
                     VPGVASARQPGTLPWAGTFHSVGARLLRQYAERIGLNDSFSILDRGDAEDLLDIVRHD
                     LGLSATKRRFPAKGTCIAIYSRVVNGEAALDDVLAQAYPWCAQWSTELAKLFEAYVMA
                     KQAQNVLDYDDLLLYWTHMLAEPSIARDVGDRFDHVLVDEYQDTNRVQSSILLALKPD
                     GRGITVVGDDAQSIYSFRAATVRNILDFPQHFQPAARVVTLERNYRSTQPILDASNAV
                     IGLAPERFTKNLWTDRASGKKPRLVTTRDEAAQACYVADRVLEERESGTALKSQAVLF
                     RSSSHSAQLELELARRAIPFVKYGGLRFLEAAHVKDVLAMLKWANNPRNRMAGFRALR
                     LLPGVGPARATRFLDALSELPDLRSAMESFTPPAALAADWSALAALLRALGESKPSWP
                     ADIDAVIRWYEPHFERIFDDAFVRRGDLVQLQQIASTYPSRERFLTELTLDPPDATSD
                     EAGPPGRDDDYLILSTIHSAKGQEWKAVHVLNVVDGCIPSDMATGSTEEVEEERRLLY
                     VAMTRAKETLELVSPQRFFVRQQSAHGDRHVYALRSRFIPDALTEFFESCASPVVTSL
                     AEPAVASGKESSAPVDIAARVRARWSGPQ"
BASE COUNT         1937 a         3821 c         3767 g         1835 t
ORIGIN      
        1 cgccgtcgat ctgcgtcttc gcccactcgg cgatgttggg aatcagcgcg agcacgaccg
       61 cgggcgcgtg gctcttcggc gtcacctgga acgcctgcgc gccgatcacg agaccgatat
      121 agagcaggat gggcaggatc gcgaccagcg gaatcaccgc cagcagaaac gcggtgaggc
      181 cgaggaagca gacgatggcg atggcgatgc cggtcgcgag cgaatagccg atgcgtccac
      241 cgacggcctt ccagcccgga tggccgatgt aaacggcggg cggaaacggg ctgccgagca
      301 ccgagccgac gatggcgccg atgccgtccg ccagcaacac cttgcgcagg ttgtagttgt
      361 cgccggcggc cgaagcgctt tcgacgttgt tcattccctc ggtgaagttg tagatgccaa
      421 gcggaatggc ggtgacgagc aacggcgcga cctgtgccag tccctgccac acgtccgccg
      481 acagcaacgg cacacgcagc ccgaattgct cgaacgactt cgcaaccgcc gcgggctgca
      541 tgtaatcgct ccagccgacc agcgacacca cccagccgat caccgagccg acgatcacgg
      601 ccgcgagccc gcccggaacg ccgccgggaa ggcggacgtt ggccgtccac gcgatcagga
      661 cgagcgcgaa gcacacgaac ccgatccatg gcacttccca catctggaac gcggggcgca
      721 tcgagatgaa ggcgatcgag atgccggcga gcgtgccgag catcgcggcg cgcggagtga
      781 gcgagcggat cttcggcccc acgaaggcgc cgatcagcac gatgacgccg atgatgaagg
      841 cccacgcgag gccggcacgc caggcgacca gcgggtcctt cgtcgcgagg tacgtcggca
      901 acatgatcag gaacacgacg atgaacatgt gcggcacgct cggaccgtac ggcagcgcgg
      961 tcacgtcggt gcggccttcc tgcttgccga gttgccaggc aaggtacgcg tagaacaggt
     1021 tgccgatcgg caacgcgatg ccgagcgccg ggaggatgcg gccgaacacg gtgtcggcgg
     1081 gaagctgaac gacgccgagg cacaagccgg tgagcacgat gacgttgagc agcacgttcg
     1141 tgaacaaacc gaagaacccg ttcaggtcgc caatcaccca cagccgggga cctgccggta
     1201 ccgcactcag tgtcgccatg ttcgtgatct ccctttacgt tgtgtgccgg cctttcgacg
     1261 ccatgtacgc gcgccagccg ccgtactccg tgatttcgcg cttgccctcg caaagaaacg
     1321 gttcgcccag cacgccgagc acgatcgatc cgtcgcgcag aacgaccttg ccgaccgcga
     1381 gccccggggg ttcggcgagc aggatgctca cgagccccgc gacggggacc tgccacacct
     1441 cgacggcgac gttcgtgcct tcaccgggcg tgcgcagcat cgccggatgc cggtcgtcga
     1501 tcgaccagat gcggtagcac gcatcggtct cgtcgtcgcg cacgaacgtc gcgccggcag
     1561 tgacgaggtt cgcgttgagc gcgagcccgc gcatcagcgt accgttgacc gcgaagagca
     1621 cgtcgttcgt cattgcacgc cccgtttcct cagctctcga tccacgtgcc ggtctcgccg
     1681 cgcaacgccc gcgcgatgtt ctccggattc gtgatcacgc cgcggccgcc gtggcgtttc
     1741 ctgacgaacg tcagcagcgc ttcgatcttg ggccgcatgc tgccttctcc gaaatggccc
     1801 tgagcggcga agcgctcggc ctgctcgatg ccgattcggt cgagccattg ctgatccggc
     1861 gctccgaagc ggatcgcgac gcgctccacg ccggtgggga tcatcaacag atcggcgctc
     1921 aattcgatgg cgagcagcgc cgacgcacga tccttgtcga cgacggcctc gacgccttgc
     1981 agttcgccac cggccgtctg ctcgaccgcg atgccgccgc cgccgcaggc gatgaccagc
     2041 acgccgtcgc gcgcgagccg cgcaacgatc ggcgcctcga cgatgcgcaa cggctgcggc
     2101 gaggcgacgc atcggcgcca tccgcgatcg ccgtcgcgca tgatcgacca gccgagctcg
     2161 cgctcgagcc cgcgcgcgat gtcctcggtc aggaacgagc cgacgggctt gtcgggatgg
     2221 gtgaacgccg gatcgtcgtg gtcgacgagt gtctgcgtca ccagcgtgac gacgggcttg
     2281 tcaatgcggc ggcggcgaag cgcgttgcct agcgcgttct ggaacatgaa gccgatcgca
     2341 ccctgcgtgt cgccgaccgc atagcggatc ggcaccgtcg gcacctgtcc atcggcgatc
     2401 tcggagcggc gcaggatgaa accgacctgc ggcccgttgc cgtgcgtcac cacgatgttc
     2461 cagcccgcaa cgaccatgtc gacgatgtgc gccgccgtcg cgcacacggc atcgtattga
     2521 tcgttgagcg cggtgtgcgc gtcatcgcgg atcagcgcgt tgccacccac cgcgacgacg
     2581 gcgatccgat cggtcatagg tcgatcatgg cgcccctcac ccgcctcgct accgctcggc
     2641 tccctctccc cgcgtgcggg gagagggttg gcgtgaggga atcgcccgca ggtcgcccgc
     2701 aagcgctttc acggccgcgc tgaagcacgc catcggcgcg gacgtgatgc cggcgccgat
     2761 ctgacccacg cctggctcgc gatgcgcgat gccgctgttg atcaccggca ggatgccgcg
     2821 atcgacgacc ttgcgcgcgt cgatgcccgc ggccaccgcg gcgaagtcga gcgcaggcag
     2881 cgtgaacgac gggtttgcgc cgagcgtgat cgcgcgcatg cgcagactgt ggtgcagcgc
     2941 gtccgccgcc gtgccgccga cgaacttcac gatcgccggc gccgcggcca tcgcgaatcc
     3001 accgacgccg gccgtctcgg tgatcgacga atcgccgaga tcggcggcgg catcggcgcg
     3061 cgagtagccg ggaaagtaga ggccgtcgac gggattcgca ggcgcttcga accatcgctc
     3121 gccggtcccc gacaggcgaa tgccgaaatt gacgccgttg cgcgacatcg cggtgaccat
     3181 cgagctcgcg ggcacgccgg ccgcggcatc gagcatcgcc ttgcacgcgg ccatcgacag
     3241 gttgaggaag aagtggtcgt tgccggcgat gaaattcagt gccgcgaggg cgcgctcgcg
     3301 cgggatgtct gaggtgagca cggccggcgt gagctgcttg aacagcagcg acgtcgccgc
     3361 ggcgttgcgg ttgtgcactt catcgcccat gttcagcgcc tgcgccatca gcggcttgag
     3421 ctcgatcctt ccgagctgcc tgacggccgc gcgcagcgcc ggtgcgacgt cgctcgcgat
     3481 gaagcgaaga cggtcgagaa cgctgcggtc gtgcgcaccg aagcgcagca ccttgccgag
     3541 accttcgttg aagttgctgt aagcgcggtt tccgcccgtc gcgttttcga cgacccacac
     3601 cggcatcgac gggctgatga tgccggccat cggtccgacg gcgccatggt ggtggcacgg
     3661 ctcgagcgcg atctttcccg catccaccag gcgcgtggcg gcgtcgatcg tgtcggccca
     3721 tccttcgagc agcacggcac ccagcaccgc gccgcgcatc ggcccgcaca tcgcggccca
     3781 cgcgatcggc ggtccggcgt gcaggatcag ccggcgaccg gagcccattg cggcgatgac
     3841 atcgcgggcg accgcgacgt cggtcagcac gggattggct tcgagaaagc gcgcgaatgc
     3901 aaccgcgttc gcgtggtcga cgactgcatc gtcgaagatc tgcgcgagcg cccagcccgc
     3961 atcgcgatcg cccagcgcgg gcggctgcca ggccgtcgcg atgcaagcac cgccggcggc
     4021 agtgacgttg tcgccgaaac cggcgaggcc gacgttgacg actttcaacg tgtccttgaa
     4081 cagcgccttc atggcattct cgccgcgtgc cgctgcgcaa gacgcagcgc gagctgcgcg
     4141 gcaagcgacg cagcctcgat gttgctgtcg gcgacgatcg cgccggcgga cgcgagcgtg
     4201 cggatctgca ccgcccggtc ctgcggatcg ccatcggtgc cgcagacgtg accgatcggc
     4261 accagcttgc gtcccccgag cgcggccgcg cgctgggcgt cgcgcaacgc gtcggcgagc
     4321 tcgtcggccg ggctcggatg cgacccgaag ccgagcacga ggtcgaacag cagcagggcg
     4381 actgccggat cgtcggcggc ttcgcggatc gcggcgttgc gcagcgacgg atcgatcatc
     4441 gggtgcggcc gcccgcgcgt gtattcgtcg tcgcccatgt cgatgaagac gtgatcggcg
     4501 gtcgacccgt cgagcggcag cgcgccatgc accggtgcgt tcgaccggca tgccaacccg
     4561 cggtggagga acgcgagctg cgcctcgtag cagaaggtcc cgccggtaaa gagcccgcgg
     4621 acggtgcgct gcgtgttcgc gacgccgtcg accacgtgga cgcgatcggc gctcgcgtcg
     4681 ttcgccgcga tggcggacgc ggacgcgcgc tcgctgcccc gcgacagcgc caccgcaatg
     4741 tcggcggcgt ccgccaatga ttgggccgca tgcaggcgcg cgccctgcac ggtaccgggg
     4801 gccgcaccga gaaaatgcac gaccgtcggc ttgcccgagc ctgcggcgag cgcgaggatg
     4861 cgcgaggcga ccgtcggcga cggcggcttc gagatcagca cgatcacgcg cgtccggtca
     4921 tcatcggcaa gtgctttcag cgcggcgagc atcgtgagcc cgccgatctc gtcacgaaga
     4981 tcgcggccgc cggttccgat cgcctggctc acaccggcgc cgcggttgtg aatgcggcag
     5041 gtcacctcct gcagacccgt gccggacgcc gcgacgaggc cgatgtcgcc gcgtcgaacg
     5101 acgttggcga aaccgagcgg gatgccgttg atgatggcgg ttccgcaatc gggacccatc
     5161 acgagcaggc cgcgctcctc cgcatggcgc ttgacagcga cttcctggtc gacgccgacg
     5221 ttgtccgaga acagcatcac gtgcaatccc agcgacagcg ccttcatcgc ctcggcggcg
     5281 gcgtagtcgc cggggacgga gatgagcgcg agatcggcat ccggcgtttg ctcgatggcc
     5341 gcggcgatgc tcgtcagcgg catcgagtag gcgtcgtcgc gttgatcggc ctctcgcggt
     5401 gcgccctgca gcagcgcctg cgcggcgtcg atcgcgtcgt cgcacgcttg cgcctcgccg
     5461 cgaacgacga ccagcagatc cgaaggatgg gcatcggcgt cgaccgacag gtcggcgtgc
     5521 tgcaactgtg cgaggttggc cggcgtggcc atgatgcagg acgcctcgac gatgccgggc
     5581 cgctgccgca gcgccgcgcc gacctgcatc agcgcgaccg agtccttgta cagattggtg
     5641 aagacccgaa acctgatctc agtcgtcatt caggcgctcg tcaaccggag gcgggaggcc
     5701 atgccgagat gcccgcaagc acaccggcag gcagatcggc accgacgtgg caccgacggc
     5761 cagcgcgcat cgtccgccat ttgagcgacc cgtgcaacat cgctaaagat gccgggccgc
     5821 agcgatgacg acccgtgtgg ccaggggaga ggccgcgctt ccggacctcg gccatgacgc
     5881 cgctgcgaaa gaagtcgtcc aggcagcgcc gctacatcgt cacgccttcc aggtccgcca
     5941 tcagcgcctg catctcggcg ccgcccgcca tcatgtcgag cacctgggcc ttcgtcacgt
     6001 cgcgcttggc gaacgtgccg agcgacttgc cgcggttcag caacgtgaac gtgtcggcga
     6061 ccgggtaggc gtggttgaca ttgtgcgtga tgaagatcac cgagattccc ttcgtgcgag
     6121 cccggtggat gagcttcagc acgttcacgc tctgcctgac gccgagcgct gccgtcggct
     6181 cgtcgagtat cagcacgcgc gcaccgaagt agatggcgcg cgcgatggcg aggcactgcc
     6241 gttcgccgcc gctcatcgtg cccacgacct ggctcgcgtc gcgcatcctg atacccattt
     6301 ccgcgagctt ctcgatcgcg gtgcggcccg cctgtgcatg gtccatcacc tggaacgggc
     6361 cgaacaaccg gcgccgcggc tcgcgtccca tgacaaagtt gcgcgcgacg ctcatcagcg
     6421 ggacgagcgc cagatcctgg tagacggtgg cgatgccgag atcgagtgcc tcgcgcggcg
     6481 acttgaagcg catccgccgc ccgtcgacga ggtactcgcc gcggtcgggc ggatgcacgc
     6541 ccgcgagcgt cttgatcagc gtgctcttgc ccgcgccgtt gtcgccgagg agacaatgca
     6601 cctcgccgcg ccgcaggcgc agcgtcacgc cgttcagcgc gatcaccgac ccgaagaact
     6661 tgctgatgtc ctcgagctgg acgatgtaat ccgcggtcat atgtaaattg gagtcacact
     6721 gtaaatcggg gtcagagatg tatttcgcgc gcgaggtctt cccagtcccg aatgccgtgc
     6781 cgcgaaatac atctctgacc ccgatttacg ctcttcaccg gcgacccagc gcgacgcgcg
     6841 tgcgcacgta cgtgttgaac accacggcgg cgagcagcat gccacccagg aagacgcggt
     6901 accagttgtt gtcgatgctg gtgtagccga tgccgatctg cacgacgccg aagatcagcg
     6961 cgccgatcga ggcgcccacg accgagccgt agccgccgct caacaggcag ccgccgatga
     7021 cggcggcgat gatcgcctcg aactccttct gcaatccgcg atcggccgcg gccgagccgg
     7081 cgtcggcgac ctgcagcacg gcgaacagtc ccgagcagag cgccgtcacg acgaacaggc
     7141 cgaccttgac gcgtgcgacc ggcacgccgg tgttcttcgc cgcattcgca tcgccgccga
     7201 cggcgaggat ccagttgccg cggcgcgttc tggcgagcac gaaaccgccg atcgcggtga
     7261 gcagcagcca ccacacgatc accttcggca cgccgatggc aagtgcgctg ccgttgtctt
     7321 tcgccgcgat ccagccccag cgcgccatcg tgtcgaagag gccggtcagc acgtggcccg
     7381 agaagagcgt cgagaacaga gggtcgcgag tcgcgaggtc gcgcagcgcc acgccctcgt
     7441 tgctgctgat gatcgtcttg ccggtgagca tcgtcgacag cgcgaggctc aggccgcgca
     7501 ggatgaagta gaacgcgagc gtgacgatga agctcggcag cccggtgcgc acgaccaggc
     7561 agccgttcag catgccgagt gccgcgcaca gcgcgaacgt gaagaccgcg gcgagccaca
     7621 gcggccaacc ccaatacagc gcgggaatcg cgagcatcat gcccgcgaag ccgatcatcg
     7681 agccgatcga cagatcgaac tcgccgccga tcatcaacag cgccgcacct acggcgagga
     7741 tgccgaggaa tgcggcgacg gtcgcccagt tgacgatgcc ctcggcactg aacatgccgg
     7801 agtcgcctgc caccagtccg aacagcacga acaccaggac cgcgccggag attgagccga
     7861 attccggacg cgccaggaag cggcgcaatg caccgatgtc gcgcacgcgc tcgtcggacg
     7921 acggcgcgtg cgtcgcgcga atgacgggcg acgaagtgtc gggaccggcc aacgccgtct
     7981 ccgtgcggcg cgtcagcgat attgcccggc gtacttctcg accttggcga tgttgtcttt
     8041 cgtgatgaag cccggacccg acgagatgtg gcgcgggcca tagttgggca cgagcccgta
     8101 ttcatcgaag cgcttcttcg tcttcggatc ggccttgacc gcgtcggcga ccttcttgag
     8161 gtcggtggtc ttcatctgct tcatcgtcgc aagcacggcc accgggatgt atccctgcag
     8221 atacggctgc tgatcgatcg cgaacttcac cgtgcccgac ttgatcgcct tgctgatgtc
     8281 gtcggacaga tcgaaggtcg ccatccagag cttgccggcc tttcccgatt tttcgagcgc
     8341 cctgatcgtg gggctggctg acgtcggtcc aagggccagc acgccgttgg tctcggggtg
     8401 ctggcgcaaa tacgcggaga ccttcgattc gatcgtcgtc gggtcgtcgc ccacgtcgag
     8461 cgtcgacttc ttgtagtcgg cgccgatggc ctcagcgaac ccgcgacagc gctcgaagct
     8521 cgccgggttc gtcgcgtagt ggttgacgca caggaagctc ttcacgcccg cggccttggc
     8581 gcgctcgcca gcacctttgc ccgcggcgta ctcgggctgc cccacgtgca tgatcgcacc
     8641 gagcttttcg ctctgctcgg tggttccgga gttgatcgtg atcagcggaa tcttcttcgc
     8701 ggtgaccttg ccgatggcgc cttgcagcac gttgtagtcg gcgatcgtcg tgatcacacc
     8761 gtcgtagtcg gcggctgccg cctgctcgat cagtcgcgcc atgtcggcga gatcgccgga
     8821 cggcgggttg cggtagtcga ccgtgacgtc gtaatcctcg cccgcctgct tgatcgaatt
     8881 cttgatcacg ttccaccatg aatcggagtc gggcgcatgg ctgatcagaa cgtacctgcc
     8941 ggccccgtag gctgttccca tcgtcaacag cgcgctgagc gcgaggatgc ccagcaaaga
     9001 tcgcttgcac gaaaacgtca tgattgcctc cagttgtggc ctcgacggga cgcggcggcc
     9061 ttcgcccgaa agccgagatg caagtgtaga cgcgcggaat cgaatactca gcggaatgcc
     9121 atctggcaaa gatcgatgtt atcctccggt cgaacgttcg ttcgctgcgc gttgcagcgt
     9181 aaacgtgcgt gcttcccgat ctcggatcca gtgactcgat gctcgcctat cggccgccag
     9241 ccgtcgcaaa acttccggca acgacaacgc acgtccccga ctacctcgct tcgctgaacg
     9301 cgcagcaacg cctagcggta gagcatggtg tcggccccac cgcgaatgac cgcggaccgc
     9361 tgctgatcat tgccggcgct ggctccggca agacgagcac gctcgcgtgt cgcgccgcgc
     9421 atctgatcgc gaacggcgcc gatccgcagc gcatcctgct gctcacgttc tcgcgccgcg
     9481 ccgccgaggc gctcgaacgg cgcgcggggc agtttctcca tcgcgtgcct ggcgtggcgt
     9541 cggcgcgcca gccgggcaca ttgccgtggg cgggaacatt ccatagcgtt ggtgcgcgcc
     9601 tgcttcgaca gtacgccgag cgcatcggtc tcaacgactc tttcagcatc ctggatcgcg
     9661 gcgacgccga ggatctgctc gacatcgttc ggcacgatct cgggctgtcg gccacgaagc
     9721 gtcgcttccc ggccaagggc acgtgcatcg cgatctattc gcgcgtcgtc aacggcgagg
     9781 ctgcgctcga cgacgtgctc gcgcaggcgt atccgtggtg cgcgcaatgg agtacggagc
     9841 tcgcgaagct gttcgaggcg tacgtcatgg ccaagcaggc gcagaacgtc ctcgactacg
     9901 acgacctgct cctctactgg acacacatgc tcgccgagcc gtcgattgcg cgcgatgttg
     9961 gcgatcgctt cgatcacgtg ctggtcgatg agtaccagga tacgaatcga gtgcaatcgt
    10021 cgattctcct tgcgctgaaa ccagatggcc gcggcatcac cgtggtcggg gacgatgcgc
    10081 agtcgatcta ttcgttccgt gcggcgaccg tgcgcaacat tctcgatttt ccgcagcact
    10141 tccagcccgc agcgcgggtg gtgacgctcg agcgcaacta tcgatcgacg cagccgatcc
    10201 tcgatgcctc caacgccgtg atcgggctcg cgcccgagcg cttcaccaag aatctctgga
    10261 ccgatcgtgc atcaggcaaa aagccgcgac tcgttacgac ccgggacgaa gccgcgcagg
    10321 cgtgttacgt cgcggaccgt gtgctcgagg agcgcgaaag tggaacggca ttgaaatcgc
    10381 aggccgtcct gtttcgaagc tcgagccata gcgcgcaact ggagctcgag ctcgcgcggc
    10441 gcgccattcc gttcgtgaag tacggaggac tcaggttcct ggaagccgcg cacgtcaagg
    10501 atgtgctggc gatgctgaag tgggcgaaca acccgcgcaa tcgcatggcg gggtttcgcg
    10561 cgctgcggct gcttcccggc gtcgggccgg ccagggcgac acgatttctc gacgcgctgt
    10621 ccgaactgcc ggatttgcgc tcggcgatgg aatcgttcac gcctcccgcg gcgctcgcgg
    10681 cggattggag tgcgttggcc gccttgttgc gtgcgctcgg agaatcgaaa ccgagctggc
    10741 ccgcggacat cgacgcggtc atacgctggt acgagccgca tttcgagcgc atcttcgacg
    10801 acgccttcgt gcgccgtggc gatctcgtcc agcttcagca gatcgcatcg acctacccgt
    10861 cgcgagaacg gttcctgacc gaattgacgc tcgatccgcc ggacgcaacc agcgacgagg
    10921 ccggtccgcc gggacgcgac gacgactacc tgatcctgtc gaccattcac tccgccaagg
    10981 gacaggaatg gaaggccgtg catgtgctga acgtggtcga cggctgcatc ccgtccgaca
    11041 tggcgacggg cagcacggag gaagtcgagg aagagcggcg gctgctgtat gtcgcgatga
    11101 cgcgcgccaa ggaaacgctg gagctcgttt caccgcagcg tttcttcgtg cgccagcaaa
    11161 gcgcacacgg agatcgtcac gtctacgcgc tgcggagtcg attcatcccg gatgctttga
    11221 ccgagttctt cgaatcgtgt gcgtcaccgg tcgttacctc attggccgaa ccggcggtgg
    11281 cctcaggaaa agagtcatcg gcgccggtgg acatcgcggc gcgcgtgcgt gcccgatggt
    11341 cgggtccgca gtgaaggatg
//
LOCUS       DASSVX010000323        14977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_898116, whole genome shotgun sequence.
ACCESSION   DASSVX010000323 DASSVX010000000
VERSION     DASSVX010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 14977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14977
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898116"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFO53_13805"
     CDS             <1..247
                     /locus_tag="VFO53_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET8877400.1"
                     /translation="DLECVDRVLHDVPLDREVVVYCNCPNEATSARAAKALMAKGYRH
                     VRPLRGGLDAWGAAGYPVQRLASATEALSGGIARTPA"
     gene            276..1244
                     /locus_tag="VFO53_13810"
     CDS             276..1244
                     /locus_tag="VFO53_13810"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877401.1"
                     /translation="MRMREIPVVMPYRLDLTVSVLRRLSSNVVDLLSPQGMYVRALSG
                     FPEPVVVRVTQIAHPHALQVAIDGDRRNDAAIVSVVGQMLGADCDLRRFYRAAAHIPW
                     LAPLVKRMRGVKQPRYPTLWEACANAIVFQQISLRAASTIMYRLIVAIGQCVQADGVP
                     VPLYLFPTAERFQHERDERLRATGLSANKIATLRRAAEAIESGALDAATLDRCTSQDA
                     AAMLRGIKGIGPWTATIILLRGLGRLDVFPGNDTSVASSIALLAGSAPLDVQRVLDDL
                     GPQRGMLYFHLLLGRLEARGDIGRPSFERSPSNDQPLASAVDDSAD"
     gene            complement(1288..2316)
                     /locus_tag="VFO53_13815"
     CDS             complement(1288..2316)
                     /locus_tag="VFO53_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8877402.1"
                     /translation="MKGAHRSWWVPPVDHISGALARNGMPATLSGFVLRVSGWSQVRV
                     SCLAIAVFVLTTAPLEMQRRILNAAVLDGDPRLVLVLAVVYAGIVLAEGLIKLLMNIY
                     RGWIGEKAVRILRLAASARVDSMPEQRTRADIQGVEISLILPEPEPIGGFVGVVASEL
                     VLQAGTLLSAFAYMLYMQPLLALACLLIFSPQFVFVPLMQAAINRRVQARINVLRQAS
                     VGVLVPGIGEAEVERVLRQQLRFAEIFSLNLGVVKLRYSMHFLMNITHNFAKIIVLGA
                     GGWYVINGETEVGTVVAFVSALNNVHDPWGALVNWYEDMMVTRTRYQTFVAAMQQFRK
                     SGEALAVA"
     gene            complement(2354..3571)
                     /locus_tag="VFO53_13820"
     CDS             complement(2354..3571)
                     /locus_tag="VFO53_13820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET8877403.1"
                     /translation="MRFAIASKTASSITLSDRAVAFAYVAAIVLAVAEVVLDLVTWVD
                     LDIAAIYGIPLVLAAITRSRRLLWTLATALTFATFMAYALQIPAGTFGLHEALFVNRV
                     LDAVALLLTAGLIQIWMMSLDVREAQARLLHEQNRKLEAANQQLVAHEAQIVRQNEEL
                     DRRRQDAEQVSGRKTQLLNAFSHDIRNPVNAINLMAEVIRRTAEDPALAMQVPQMARR
                     LQSNAQSLVALVSEVLDIAHLDSGLLQRRESTFSLNGFIEAKCRDLAPLAEAKSLHLA
                     PENPERAVDVRTDQVKLDRIITNLITNAIKFTATGNVTVSAQIAADGAAMIRVRDTGV
                     GIAEHELKHIFDEFAQFNVPLGKPSGGWGLGLAISRRLANFIGARVSVESALGHGSVF
                     TLTLPAECVVRDT"
     gene            3861..5078
                     /gene="sat"
                     /locus_tag="VFO53_13825"
     CDS             3861..5078
                     /gene="sat"
                     /locus_tag="VFO53_13825"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HET8877404.1"
                     /translation="MSVLVPPHGGRGLRPLQLQGERRAQESARARTLRNIPVTSREKG
                     DLVMLGIGGFTPLDGFMTHADWKGVCDEMRTADGLFWPIPITLSADATTADSIGIGED
                     VALVDPDDASVLAVLRVSEKYRIDKAHECQSVFRTTDTAHPGVQMVMRQGDVNLAGSV
                     RVLGDGGFGASYGALFMTPAQTRAEFERLGWSRVAAFQTRNPMHRSHEYLAKIAVEVC
                     DGVLVHSLLGHVKAGDIPADVRTRAIGVLIDRYFRAGTVLQAGYPLDMRYAGPREALL
                     HALFRQNYGCAYQILGRDHAGVGSYYGPFDAHRIFDEIPRNALAIEPLKIDWTFWCYK
                     CGGMASARTCPHDDADRLLVSGTKLRKWLSEGSPVPPEFSRPEVLDILRAYYASLAAH
                     ERVEVKLSGHSAR"
     gene            complement(5122..6048)
                     /locus_tag="VFO53_13830"
     CDS             complement(5122..6048)
                     /locus_tag="VFO53_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET8877405.1"
                     /translation="MADRRLQAFHAVAKHRSFTKAADALCMTQPAVTFQIRQLEEQFN
                     TRLFDRVNGRITLTPAGTIALDYAERILGLSAELNTRLKEVSGQEVGPLLIGASTTIA
                     EFLLPRILGEYKVRHPGIVPRLSVANSEAVQARVAERSLDLGFIEGDSHLPTLVTDVC
                     CQDELQVVCVPSHPLAREKAVALKALCEHAYISREAGSGTREVIDHYLQKGGLSPDAL
                     QIVMEASSPEALKGLAATGLGFAIMSRTTAEKEARLGELVQIPLAPPLLRHLSVVYPK
                     ERFHSRIVNGFVAFAKERLGGARMSGDRLAAD"
     gene            complement(6143..6658)
                     /locus_tag="VFO53_13835"
     CDS             complement(6143..6658)
                     /locus_tag="VFO53_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET8877406.1"
                     /translation="MKLCIVSDSHDRAEPLADAVREAAAQGAAAVIHCGDVIGTQTLR
                     AAMSVGLPLHVIHGNNLGDPVSLSRWARESDGMLQYHGVDARIELAGRRVFVVHYPEY
                     GYAMACTGDWDLVCCGHSHVAAIDRVRNVKQRETWLVNPGTVAGLAAPATWILGDLDT
                     MRFELRTLAPG"
     gene            complement(6687..9032)
                     /locus_tag="VFO53_13840"
     CDS             complement(6687..9032)
                     /locus_tag="VFO53_13840"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET8877407.1"
                     /translation="MNNSGAQQPSSIDPVCGMRVAASSSHRYTFRDSEYRFCSARCAE
                     RFAAAPEQYLKSASSASGTSGTPADPSTPSASSAAQAAITATIYTCPMHPAIRRPGPG
                     NCPICGMALEPEMPTATEERNPEFEDFKRRFWWTLPLTLVGVALAMGAHAVMPVSPAA
                     RSWIELVLSTPVVLWAGWPFFVRCVDSIRHRSPNMWTLIGIGVAAAYVYSVLATVAPQ
                     LFPAEFRAAGRVGVYFEAASVIVSLTLLGQLLELRARSQTSAAIRALLGLQPKTARRI
                     ASDGTEADVELSDVQVGDILRVRPGEKVPVDGTATDGSSTVDESMLTGEPIPVEKRAG
                     AKLIGATINGTGSLVMRAEKIGSQTVLAQIVQMVAQAQRSRAPTQRLADVVARWFVLA
                     VLGVAVLTAFAWWLLGPEPRGVYAVLNAVSVLIIACPCALGLATPMSIMVATGRGATS
                     GVLFRDAEALETLRRVDTLIVDKTGTLTLGRPVFARAIGVAPYADDEVLRIAASIDQG
                     SEHPLANAIVQAARERGLLLAKPENFESSTGIGVRGSIEGRAVALGNAVLMREAGADT
                     ALLGADAERLREDGGSVVFLAVDGRLAGLLAVHDPVKESTSAAIAALHDAGIHVIMAT
                     GDGVATAKAVARRLGIDDFRGEVRPQDKADLVADLQQKGRVVAMAGDGINDAPALARA
                     NVGIAMGTGTDVAMSSAGITLVKGDLRGIARARALSEATVRNMYQNLSFALVYNAVGV
                     PVAAGVLYPFLGLLLSPLIAAAAMSLSSVSVISNALRLRSA"
     gene            complement(9085..9798)
                     /locus_tag="VFO53_13845"
     CDS             complement(9085..9798)
                     /locus_tag="VFO53_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET8877408.1"
                     /translation="MTRRFRLHWRCSAIVALAVLPIAERALADGDATRGATLFEACAA
                     CHSTTAGVQMTGPSLANVWGRKAGTVAGFTRYSDAMKRADVVWNDGTLDRWLTNPDAL
                     IRGTSMTFPGIRESQARRDVIAYLRAVSDGKAPPAKGRGGMVMNMASPKEDLRKAPPE
                     GQVASIMHCKDTYTIKTADGKLNKVWEFNLRLKTDSSDLGPLPGKPVIVGAGMQGDRA
                     SVVFAAPSEISRFITEQCP"
     gene            complement(9842..10531)
                     /locus_tag="VFO53_13850"
     CDS             complement(9842..10531)
                     /locus_tag="VFO53_13850"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET8877409.1"
                     /translation="MSFADRSLIAETTAKMLLEIEAVLFNAEKPFIFTSGWASPVYID
                     CRKLISYPRVRRTLIDFSCSTIFREIGFESVDTVCGGETAGIPFAAWIAEALALPMQY
                     VRKKAKGFGRNAQIEGHLADGARALLVEDLTTDGRSKINFCEALRGAGARVDHVFVIF
                     YYAIFAESEKILADLGVTHHCLATWWDVLAAAKKYRYFDTNTLGEVERFLHQPGEWSA
                     AHGGAARSAGA"
     gene            complement(10580..11569)
                     /locus_tag="VFO53_13855"
     CDS             complement(10580..11569)
                     /locus_tag="VFO53_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET8877410.1"
                     /translation="MTPNDSAPVARLDAGPETVHWGYFDARLAPVLTIESGERVVIST
                     VSGAPDLLPPPPFAYPPALPAIHAANGPQRFFGHMCTGPVAIRGAKPGQTLRVDIEAV
                     EPHYDWGHNVVRPQAGALPDDFTETRVTHIALDRERGVWRLPWGQDVPFRPFFGVMAV
                     APPRAWGAIPTPPPRRNGGNIDNKELVAGSTLFLPIFNDGALFSVGDGHGAQGDGEVC
                     VTAVETGLIGTFRLTLVDDMPLEWPRAETPTHMITMAFDPDLDVCVKIVLRQMLDVVC
                     ERAGLDRYQAFSLLSLAGDLRVTQVVNGNKGIHLMLDKRYLARPQHRGTHAVP"
     gene            complement(11636..12412)
                     /locus_tag="VFO53_13860"
     CDS             complement(11636..12412)
                     /locus_tag="VFO53_13860"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HET8877411.1"
                     /translation="MLKENARPDASRATRRQPVDSARQGPASETASRTLAGEMFERLR
                     VDILSARLPPGTKLRFDYLRKTYKVGLSPLREALSRLAENRLVVATGQRGFRVPSVSA
                     HDIIDIAMVRKEIEGLALRLSITYGDDAWEANVVAARHKVALLEKAGKNVAEDIWESR
                     HRDFHRTLVSACQSDCLLHLHALLSDQFDRYRRLSAKSRLPNSPRSLIHQRILDAVLN
                     RNADLAVRLLCDHIDEATRLIVAGLSPAEARAKRKSVARS"
     gene            complement(12479..13729)
                     /locus_tag="VFO53_13865"
     CDS             complement(12479..13729)
                     /locus_tag="VFO53_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HET8877412.1"
                     /translation="MQKIELANREALRRILAAQPVLVDVQPAHAVIEGLGRNVILHAG
                     PPITWDRMCGPMRGAVCGIAVFEGWAAGLDDAAKAAAAGEFSFHPNHHFGAVGPMTGM
                     TTASQPVMIVENGAFGNRAYCTINEGLGKVMRFGGNDGEVLARLRWIADVLGPALSSA
                     LTLRGGLPLKGIIARGLAMGDEMHQRNVACSALFLREIAPALARACADRDALADALGF
                     IGRNDQFFLNIAMAMGKSMTDPAGGIEASTIVTAMCRNGTDFGLRVSATGDRWYTAPV
                     EMPVGLFFPGYSQDDANPDMGDSAIVETIGLGGFAMATAPAVAGFVGAGSASEAANYT
                     NAMYEITEGRNPEWTMPNLDFAGVPTGIDIRKVLQTGKTPVINTGIAHKDAGVGQVGA
                     GVVRAPLACFEQALSGVAESMGIS"
     gene            13982..14944
                     /locus_tag="VFO53_13870"
     CDS             13982..14944
                     /locus_tag="VFO53_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET8877413.1"
                     /translation="MSATGRIPNRLVIAIGGNATHPEGIRGTPSEQVEVAAKLGDALL
                     PVMELGNELIITHGNGPVVGKILMRQSIARQRVEPMSLDICVAHSQGGIAYLLMQALE
                     NALRAKGNPRHVVCLLTQVEVDPNDPAFADPSKPIGYFYSEQEARALNAELGWQMRED
                     SGRGWRHVVASPHPKHIVDISLVQTVAKSGAVVIAGGGGGVPVVRTGKGQRHGVEAVI
                     DKDRTSALMANVLHIDDLMILTAVPRVAINFGKPDQQALDRASLSQVRKWHAEGQFPP
                     GSMGPKIDAAIQFLQGGGRRVMIGRLEDAMPILRGDAGTHIVAD"
BASE COUNT         2540 a         4942 c         4955 g         2540 t
ORIGIN      
        1 ggacctcgaa tgtgtcgacc gcgtactgca tgacgtgccg ctcgaccggg aagtggtggt
       61 ctactgcaac tgcccgaacg aggcgacgtc tgcgcgtgcc gcgaaggcgc tgatggccaa
      121 aggctaccgg catgtgcgcc cgttgcgggg aggactcgac gcgtggggtg cggcaggcta
      181 tccggttcag cggctggcgt cggcaaccga agcattgtca ggcggtatcg cccggacacc
      241 ggcctgagtt gcggtaacga agcgcggaag agccgatgcg gatgcgcgag atccctgtcg
      301 tgatgccgta ccgtctcgat ctcacggtaa gcgtacttcg gcggctttcg agcaatgtcg
      361 tcgatctgct atcgccacaa ggcatgtacg tgcgcgcgct gtcgggcttt ccggagcccg
      421 tcgtcgtgcg cgtcacgcag atcgcgcacc cgcacgcgct ccaggtcgca atcgacggtg
      481 acaggcgcaa cgacgccgcc atcgtcagcg ttgtcggcca gatgctcgga gccgattgcg
      541 acctcaggcg attctatcga gcggctgcac acatcccgtg gctggctccg ctggtcaagc
      601 gcatgcgagg ggtcaagcaa ccgcgctatc cgacactctg ggaagcgtgc gcgaacgcga
      661 tcgttttcca gcagatcagt ctccgcgcgg cgagcacgat catgtaccgg ttgattgttg
      721 ccatcgggca atgcgtccag gcggatggcg tgccggtgcc cttgtacctg tttccgacgg
      781 ccgaacgctt ccagcacgaa cgcgacgagc ggttgcgggc aaccggtctc agcgccaaca
      841 agattgcgac gttgcgccgc gccgcggaag caatcgaatc cggtgcgctc gatgcggcaa
      901 ccctcgaccg gtgcacgagt caggacgcgg cggcgatgct gcgcgggatc aagggcatcg
      961 gcccgtggac cgcgacgatc attcttctgc gtggccttgg gcgactggac gtattcccgg
     1021 gcaatgacac cagcgttgcg agcagcatcg cgctgttggc cggatcggca ccgcttgacg
     1081 tacagcgtgt gctggatgac ctcggtccgc aacggggaat gctgtacttc catctgttgc
     1141 tcgggcgcct ggaagcccgc ggtgacatcg gccgaccatc gttcgaaaga tcaccctcga
     1201 acgatcaacc tcttgcctct gccgtggacg attctgccga ttgaccagta agcggtacgt
     1261 ccgtcgggat ggcgcgagtc gcccggatca ggcgacggcg agcgcttccc cgcttttgcg
     1321 gaattgctgc atcgccgcga cgaacgtctg gtagcgggta cgggtcacca tcatgtcctc
     1381 gtaccagttc accagggcgc cccacgggtc gtgcacgttg ttcagcgccg acacgaacgc
     1441 gaccaccgtt ccaacttcgg tctccccgtt gatcacatac catccgcctg cgccgagaac
     1501 gatgatcttc gcgaaattgt gcgtgatgtt catcaggaaa tgcatcgaat acctgagctt
     1561 cacgacaccg agattcagcg agaagatttc cgcgaatcgc aactgctgcc tcaggactcg
     1621 ctcgacctcg gcttcgccga tgccggggac gagcacgccg acgctcgcct ggcgcagcac
     1681 gttgatccgg gcctgcactc tgcggttgat cgccgcctgc atcagcggaa cgaacacgaa
     1741 ctgcggcgag aaaatcagca ggcacgccag cgcgagcagc ggctgcatgt acaacatgta
     1801 cgcgaaagcg ctcagcaggg tgccggcctg gaggacgagc tccgaggcga ccacgccgac
     1861 gaaaccgccg atgggttccg gctcgggcag gatcagggaa atctcgacgc cctggatgtc
     1921 ggcgcgagtg cgctgctccg gcatcgagtc gacccgcgca ctggcggcga gacgcaggat
     1981 acgtactgcc ttttcgccga tccagccgcg atagatgttc atcagcagct tgatgagacc
     2041 ctcggcgagc acgatgcctg catagacgac tgcgaggacc agcacgagcc tcggatcgcc
     2101 atcgaggacg gcggcgttga ggatcctccg ctgcatttcg agcggcgccg tcgtcagcac
     2161 gaagacggcg atcgcgaggc acgagacccg gacctggctc cagccgctca cgcgcagcac
     2221 gaaaccagac agcgtcgccg gcatgccgtt gcgcgcgagc gctccggaaa tgtggtcgac
     2281 cggcggcacc caccacgagc gatgcgcccc tttcacgaag gcggggcgcg cggagcgctc
     2341 gagcgtcgcc ggttcacgtg tcgcgaacca cgcattccgc tggcagcgtg agcgtgaaca
     2401 cgctgccgtg gccgagcgcg ctttcgacgc tgacgcgggc gccgatgaaa ttggcgagcc
     2461 gccgcgagat cgcgaggccg agcccccacc cgccactcgg ttttccgagg gggacattga
     2521 actgcgcgaa ctcgtcgaaa atgtgcttca gctcgtgctc cgcgatgccg acaccggtgt
     2581 cgcgaacccg gatcatggcg gcgccatccg ccgcgatttg cgcgctgacc gtcacattgc
     2641 cggtcgcggt gaacttgatg gcgttcgtga tgaggttggt gatgatgcga tcgagcttga
     2701 cctgatcggt ccgcacgtca acggcccgtt cgggattctc gggcgcgaga tgcagcgatt
     2761 ttgcctcggc gagcggcgcg aggtcgcgac atttcgcttc gatgaacccg ttgagcgaga
     2821 acgtgctttc gcgccgctgc aggagaccgg agtcgaggtg ggcgatgtcg agcacttccg
     2881 acacgagcgc aacgagcgac tgcgcattgg actgcagacg cctcgccatc tgcgggacct
     2941 gcatcgcgag cgcgggatcc tcggcggtac ggcggatcac ctcggccatg aggttgatgg
     3001 cgttcaccgg attgcgtatg tcatgcgaga acgcgttcag gagctgcgtt ttgcgaccgc
     3061 tcacctgctc ggcgtcctgg cggcgccggt ccagctcctc gttctgccgg acaatctgcg
     3121 cttcgtgggc gacgagctgc tgattggcgg cttcgagctt ccgattctgc tcgtgcagca
     3181 gacgtgcctg cgcctccctt acgtcgagcg acatcatcca gatctgaatc agccccgcgg
     3241 taagaagcag cgcgacagcg tccagtacac ggttgacgaa caatgcctcg tgcagtccga
     3301 aggtgccggc cggtatctgc aaggcataag ccatgaacgt cgcgaacgtg agcgccgtgg
     3361 ccagcgtcca taaaagccgg cgactgcggg tgatggctgc gaggaccagg gggatgccgt
     3421 aaatcgctgc aatgtcgagg tcgacccagg tgacgagatc caggaccacc tcggcgaccg
     3481 cgagcacgat cgccgcaacg tatgcgaagg caaccgcgcg gtcggaaagc gtgatggacg
     3541 aggccgtttt cgatgcgatg gcgaaacgca ttcttgttgg cacggatgga cgattccggc
     3601 gtcccgacgg gcggcgtcga tgaacgcaaa cgcccggcca cacgctacga tatcgcgaaa
     3661 acgcaacgca cggcgtaggg gtctcttgac tgccgatgta agcgtcgagc cagcgttttc
     3721 gggcaatgca ataaccacga catcaccgtt ggtacctgtt ccgccatcga gcgtctttat
     3781 cgcggcatca gcaattttgt ccgacacccc gccgcacgtg cgctaacgta gctgcggtct
     3841 tcgccaccat tcatatcatc atgtcggttc tcgttccacc gcatggcggc cgcggtctgc
     3901 ggccgctgca gctgcaaggc gagcgtcgcg cgcaggagtc ggcccgcgcc cggacgctgc
     3961 gcaatatccc cgtcacgtcg cgcgaaaagg gcgatctcgt gatgctcggc atcggcggct
     4021 tcacgccgct cgacggcttc atgacgcatg ccgactggaa gggcgtgtgc gacgaaatgc
     4081 gcaccgccga cgggctgttc tggccgatac cgatcacgct gtcggcggac gcgacaaccg
     4141 ccgattcgat cggcatcggc gaggacgtgg cgctcgtcga tcccgatgac gcgagtgttc
     4201 ttgccgtgct ccgcgtcagc gaaaaatatc gaatcgacaa ggcgcatgag tgccagtcgg
     4261 tgttcaggac gaccgacacc gcgcacccgg gcgtgcagat ggtgatgcgc cagggcgacg
     4321 tcaatctcgc cggcagcgtg cgcgtgctcg gcgacggcgg attcggcgcg agctacggcg
     4381 cgctgttcat gacgccggcg cagacgcgtg ccgaattcga gcggctcggc tggtcgcgcg
     4441 tggccgcgtt ccagacgcgc aatccgatgc atcgctcgca cgaatacctc gcgaagatcg
     4501 cggtcgaggt ctgcgacggc gttctcgtgc attcgctgct cggccacgtg aaggccggcg
     4561 acatcccggc cgacgtgcgc acgcgggcga tcggcgtgct gatcgatcgc tacttccgcg
     4621 ccggtacggt gctgcaggcc ggctatccgc tcgacatgcg ctacgcgggt ccgcgcgagg
     4681 cgctgctgca cgcgctcttt cgccagaatt acggctgcgc ctaccagatc ctcggccgcg
     4741 atcacgcggg tgtgggcagc tattacggcc cgttcgacgc gcatcggatc ttcgacgaga
     4801 tcccgcgcaa cgcgctcgcg atcgagccgc tgaagatcga ctggacgttc tggtgctaca
     4861 agtgtggcgg aatggcgtcg gcacgcacgt gcccgcacga cgatgccgat cgcttgctcg
     4921 tttcgggcac gaagcttcgc aagtggctgt ccgaaggcag tccggtgcct ccggagttca
     4981 gccggccgga agtgctcgac atcctgcgcg cgtattacgc gtcgcttgcc gcgcatgaaa
     5041 gggtcgaggt gaagctcagc ggccattccg cgcgctagcc gtccgcggca cgctcgcgcc
     5101 ccgtctccgc aggcgcgacg atcaatcggc cgccagccgg tcgccgctca tgcgcgcgcc
     5161 gccgagcctt tccttcgcga acgcgacgaa accgttgacg atcctcgagt ggaaccgctc
     5221 cttgggatag accacggaca agtggcggag cagcggtggt gcgaggggaa tctgcacgag
     5281 ctcgcccagg cgagcctcct tttccgcggt cgtgcgcgac atgatcgcga agccgagccc
     5341 ggtggccgcg agccccttga gcgcctcggg actgctcgct tccatgacga tctgcagcgc
     5401 atcaggcgaa agtccgccct tctgcagata gtgatcgatg acttcccgcg tgcccgaccc
     5461 cgcctcgcga ctgatgtagg cgtgctcgca cagcgctttc aacgccacgg ctttttccct
     5521 tgcgagcggg tgcgaaggca cgcacacgac ctgcagctcg tcctggcagc acacgtcggt
     5581 cacgagcgtc ggcaaatgcg agtcgccttc gatgaatccg agatcgagcg agcgctcggc
     5641 aacgcgcgcc tgcaccgcct ccgagttcgc cacgctgagc cgcggcacga ttcccgggtg
     5701 gcgcaccttg tattcgccaa ggatcctcgg cagcaggaac tcggcgatgg tcgtgctcgc
     5761 gccgatcaag agcggtccca cttcctgccc gctgacttcc ttcaatcgcg tgttgagctc
     5821 ggcggacagc ccgagaatgc gctccgcgta gtcgagagcg atcgtgcccg cgggagtcag
     5881 cgtgatgcgt ccgttcaccc ggtcgaagag gcgcgtattg aattgctcct cgagctggcg
     5941 aatctgaaac gtcaccgccg gttgcgtcat gcacagcgcg tcggccgcct tcgtgaacga
     6001 ccggtgcttg gcgacggcgt gaaaagcctg caggcgacga tccgccatgg gtgcattcgc
     6061 tgtggtcgat ggtcagtgcg catcatacgc ggccgacgtc ggcgccctcg cactcgcgct
     6121 cgagcggctc cgcgtacttg gatcatcccg gcgcgagggt gcgcagctcg aagcgcatcg
     6181 tgtcgaggtc gccgagaatc cacgtggcag gcgccgcaag ccccgccacg gtgccgggat
     6241 tcacgagcca cgtctcgcgc tgcttcacgt tgcgcacgcg gtcgatggcg gccacgtgcg
     6301 agtggccgca gcacacgaga tcccagtcgc cggtgcatgc catcgcgtat ccgtactcgg
     6361 gatagtgaac cacgaaaacc cggcgtccgg cgagctcgat gcgcgcgtcg acgccgtgat
     6421 actgcagcat gccgtcgctt tcgcgcgccc agcggctgag cgagacggga tcgccgagat
     6481 tgttgccgtg gatcacgtgc agcggcaatc cgacgctcat ggcggcgcgc agcgtttgcg
     6541 tgccgatgac gtcaccgcaa tggatcaccg cggcggcgcc ttgcgccgct gcctcacgca
     6601 ccgcgtcggc gagcggctcg gcgcggtcgt gactgtcgga cacgatgcat agcttcatcg
     6661 gccgatgata gccgatggtg gcgcgatcat gcggaccgca aacgcaacgc gttgctgatg
     6721 accgacaccg agctcagcga catcgcggcc gcggcaatca gcggcgacag cagcaggccg
     6781 aggaacggat agaggacgcc ggctgcgacc ggtacgccga cggcgttgta gacgagcgcg
     6841 aacgacaggt tctggtacat gttgcgcacc gtcgcctcgg acagcgcacg cgcacgcgca
     6901 atcccccgca gatcgccctt cacgagggtg atgcccgcgc tgctcatcgc cacatcggtg
     6961 cccgtgccca ttgcgatgcc gacgttggcg cgcgcgagtg caggggcgtc gttgatgccg
     7021 tcgcctgcca tcgcgacgac ccgacccttc tgctggagat cggcaacgag atcggccttg
     7081 tcctgcggac gcacttcgcc ccgaaagtcg tcgatgccaa gccgccgcgc gacggccttc
     7141 gcggtcgcga cgccgtcgcc cgtggccatg atcacgtgga taccggcgtc gtgcagcgcg
     7201 gcgatggccg cggatgtcga ctccttgacc ggatcgtgca cggccaggag tccggcgagc
     7261 cggccatcca ccgcgaggaa gacgacgcta ccaccgtctt cgcgcagccg ctcggcgtcc
     7321 gcgccgagca gcgccgtgtc ggcgcctgcc tcgcgcatca ggaccgcatt tccgagcgcc
     7381 accgcgcgtc cttcgatgct gccgcgcacg ccgatgcccg tcgaagattc gaagttctct
     7441 ggcttcgcaa gcaataaacc gcgctcgcgt gccgcttgaa cgattgcgtt ggccagcgga
     7501 tgctcgcttc cctgatcgat gctggccgcg atgcgcagga cttcgtcatc ggcgtacggt
     7561 gcaacgccga tggcccgcgc gaacaccggc cggccgagcg tgagcgtgcc ggtcttgtcg
     7621 acgatcagcg tgtcgacgcg ccgcaatgtt tcgagcgctt ccgcgtcgcg aaacagcacg
     7681 ccgctcgttg ccccccgtcc ggtggcaacc atgatcgaca tcggcgtcgc gagaccgagc
     7741 gcgcacggac acgcaatgat cagtaccgac accgcgttca gcacggcgta gacgccgcgc
     7801 ggctcgggtc ccaggagcca ccatgcgaac gcggtgagca cggcgacgcc caataccgcg
     7861 agcacgaacc agcgggcgac gacgtcggcg agacgctggg tcggcgcgcg gctgcgctgc
     7921 gcctgcgcca ccatttgcac gatttgcgcg agcacggttt gcgaaccgat cttctccgcc
     7981 cgcatgacga ggctgcccgt accgttgatc gtggcgccga tcagtttcgc tcccgcccgt
     8041 ttctcgaccg gtatcggctc gcccgtcagc atcgattcgt cgacggtcga gctgccgtcg
     8101 gtggcggttc cgtcgaccgg caccttctcg ccgggacgca cacggaggat gtcgcccacc
     8161 tgcacatcgg atagctcgac atccgcctcg gtgccatcgg acgcgatgcg tcgcgccgtc
     8221 ttcggctgca agcccagcag cgcgcggatc gctgccgacg tctgcgagcg tgcgcgaagc
     8281 tcgagaagct ggccgagcaa tgtcaacgag acgatcaccg acgccgcttc gaaatacacg
     8341 ccgacgcggc ccgcagcgcg aaattcggcc ggaaagagct gcggcgcgac ggtagcgagc
     8401 acgctgtaga cgtacgccgc cgcgacgccg atgccgatca gcgtccacat gttcgggctg
     8461 cgatggcgga tcgagtcgac gcagcgcacg aagaatggcc agccggccca tagaacgacg
     8521 ggcgtcgaca ggacgagctc gatccacgac ctcgcggcgg gggagaccgg catgacggcg
     8581 tgcgcgccca tcgcgagtgc gacaccgaca agcgtgagcg gcagcgtcca ccagaagcgc
     8641 cgcttgaagt cctcgaattc cgggttgcgc tcctcggtgg ccgttggcat ctcgggctcg
     8701 agcgccatcc cgcagatcgg gcagttgccc ggacccggcc ggcgaatggc cggatgcatc
     8761 ggacaggtgt agatcgtcgc agtgatcgcg gcttgcgcag cgctcgaagc gctcggcgtg
     8821 ctcggatcgg caggcgtgcc cgaagtgccg gaagcgctcg aggcgctttt caggtactgc
     8881 tcgggggccg cagcgaagcg ctcggcacaa cgcgcgctgc aaaagcgata ttcgctgtcc
     8941 cggaacgtgt agcggtgcga cgaagatgca gccacccgca tcccgcacac gggatcgatc
     9001 gatgacggct gctgcgcgcc gctgttgttc acggcctggt gtccggtccg ttcgttgatc
     9061 gtttcgccct cgtcgtggtg acgttcatgg gcattgttcg gtgatgaacc ggctgatctc
     9121 cgacggcgcg gcaaagacca ccgacgcccg atcaccctgc atgccggcgc cgacgatgac
     9181 cggttttccg ggcaaaggcc ccaggtcgct cgaatcggtt ttcaagcgca gattgaactc
     9241 ccataccttg ttgagcttcc cgtcggccgt tttgatcgtg tacgtgtcct tgcaatgcat
     9301 gatcgatgcg acctgaccct ccggcggcgc cttgcgcagg tcttccttcg gggatgccat
     9361 gttcatcacc atgccgccgc gtccctttgc cgggggcgcc tttccatcgg agacagcacg
     9421 caggtacgcg atcacgtccc ggcgcgcctg gctttcgcgg attcccggga acgtcatgct
     9481 cgtgccacgg ataagagcgt ccggattggt gagccatcga tccagcgtgc cgtcgttcca
     9541 gacgacgtcg gcgcgcttca tcgcgtccga atagcgtgtg aagccggcga ccgttcccgc
     9601 cttgcgtccc catacgttcg cgaggctcgg gccggtcatt tgcacgccgg cggtcgtcga
     9661 gtggcacgcc gcgcacgcct cgaacagcgt cgctccgcgc gtggcatcac cgtcggccaa
     9721 agctctttcg gcgatcggca gcaccgcgag cgcaacgatc gccgaacagc gccaatgcaa
     9781 tctgaatcgc ctcgtcacgc ggaactcctg tgtcggcgcg cagcgcgaaa gcggcgtgcc
     9841 gctaggcgcc cgccgaccgc gcggcgccgc cgtgggcggc cgaccactcg cccggctgat
     9901 gcaggaagcg ctcgacttcg ccgagcgtat tcgtgtcgaa atagcggtac ttcttcgccg
     9961 ccgcgagcac gtcccaccag gtggcgaggc aatggtgagt gacgccgagg tcggcgagga
    10021 tcttctcgct ctcggcgaag atcgcgtaat agaaaatcac gaacacatgg tcgacgcgcg
    10081 cgccggcgcc gcgcagcgct tcgcagaagt tgatcttgct gcgtccgtcg gtggtgaggt
    10141 cctcgacgag cagcgcgcgc gctccgtcgg caagatggcc ttcgatctgc gcgttgcggc
    10201 cgaatccctt cgccttcttg cgcacgtact gcatcgggag cgccagcgct tcggcgatcc
    10261 acgcggcgaa cgggatgccc gcggtttccc cgccgcagac ggtgtcgacc gactcgaaac
    10321 cgatttcacg gaatatcgtc gagcagctga agtcgatcag cgtcctgcgc acccgcgggt
    10381 aggaaatgag cttgcggcag tcgatgtaga cggggctcgc ccagcccgac gtgaagatga
    10441 acggcttctc ggcattgaac agcaccgcct cgatctcgag gagcatcttc gccgtcgttt
    10501 cggcgatgag ggaacgatcc gcaaagctca cgccgccggc catgtacgca ctcgcctgtc
    10561 catcgaatgc gcaattatgt catggcaccg cgtgggtgcc ccgatgctgc ggacgggcga
    10621 gatagcgctt gtcgagcatc aggtgaatgc ccttgttgcc gttgacgacc tgcgtcacgc
    10681 gcagatcgcc ggcgaggctc aacagcgaaa aggcctggta gcggtcgaga ccggcacgct
    10741 cgcagacgac gtcgagcatc tgccgcagca cgatcttcac gcagacgtcg agatcgggat
    10801 cgaaggccat cgtgatcatg tgcgtcggcg tttcggcgcg cggccactcg agtggcatgt
    10861 catcgacgag ggtcaggcga aaggtgccga tcagtcccgt ctcgaccgcg gtcacgcaca
    10921 cctcgccgtc gccctgcgca ccgtgtccgt cgccgacgga gaagagcgcg ccatcgttga
    10981 aaatcggcag aaaaagcgtc gagccggcga cgagctcctt gttgtcgatg ttgccgccgt
    11041 tacgtcgcgg cggcggcgtc ggaatggcgc cccacgcgcg cggtggcgcg acggccatca
    11101 cgccgaagaa cggccggaac ggcacgtcct ggccccacgg cagacgccac acgccgcgct
    11161 cgcgatcgag cgcaatgtgc gtgacgcgag tttccgtgaa gtcgtcaggc agcgcgccgg
    11221 cctgcggccg caccacgttg tggccccagt cgtaatgcgg ctcgaccgct tcgatatcga
    11281 cgcgcaacgt ctggcccggc ttcgccccgc gtattgcgac aggacccgtg cacatgtggc
    11341 cgaagaatcg ttgcggaccg ttcgcggcgt ggatcgcggg cagcgcaggc ggatacgcga
    11401 acgggggcgg gggcaggaga tcgggtgcgc cggaaacggt cgaaatcacc acccgctcgc
    11461 cgctttcgat cgtgagcacc ggcgcgagcc gggcgtcgaa gtagccccag tgaacggttt
    11521 cggggccggc atcgagacgc gcaaccggcg cgctgtcatt gggcgtcatg gcttcaccct
    11581 cgttcgtcat gattcacgtg cagcactcgc ggcgaaccgg tacggcactg cagattcagg
    11641 agcgcgccac gctctttctt tttgcgcgcg cttccgccgg cgacagtccg gccacgatca
    11701 gccgcgttgc ttcgtcgatg tggtcgcaca gcagcctcac ggcgagatcc gcgttgcggt
    11761 tgaggaccgc atcgaggatt cgctgatgaa tgagcgagcg cggtgagttg ggcagccggc
    11821 tcttcgccga caggcgccgg taacggtcga actgatcgct caaaagagca tgcaggtgca
    11881 gcaggcagtc ggactggcag gcggaaacga gcgtgcgatg gaaatcgcga tgccggctct
    11941 cccagatatc ctcggcgacg ttcttgccgg ccttctcgag cagcgcaacc ttgtggcgcg
    12001 cggcgaccac gttcgcctcc cacgcgtcgt cgccgtaggt gatcgacagg cgcagcgcca
    12061 ggccttcgat ttccttgcgc accatcgcga tgtcgatgat gtcgtgcgcc gacaccgatg
    12121 gaacgcgaaa gccgcgctgc ccggtggcca ccaccagccg gttttcggca agccgcgaca
    12181 acgcctcgcg cagcgggctc aatccgacct tgtacgtctt gcgcagatag tcgaagcgca
    12241 gcttggttcc gggtggcagc cgggcgctca ggatatcgac gcgcaaccgt tcgaacatct
    12301 cgccggccag cgtgcgcgac gcggtttcgc tcgccggccc ctgccgcgcc gaatcgaccg
    12361 gctgcctgcg ggtggcccgg cttgcgtcgg gacgcgcgtt ttccttgagc atcgtgggtt
    12421 cccggtcagc gctgcgatcg gcggcgcaca cgcgcatcct gcgtggtggc gcgggatttc
    12481 acgagatacc catgctttcg gcgacacccg aaagcgcctg ttcgaagcat gcgagcggtg
    12541 cccgcacgac gccggcgccg acctggccga cgcccgcatc cttgtgcgca atgccggtgt
    12601 tgatcaccgg tgtcttgccg gtctgcagca ccttgcggat atcgatgccg gtagggacgc
    12661 cggcgaaatc gaggttgggc atcgtccatt cgggattgcg cccctcggtg atttcgtaca
    12721 tcgcgttggt gtagttcgcc gcttcgcttg ccgaaccggc gccgacgaag ccggcgaccg
    12781 cgggtgcggt ggccattgcg aatccaccga gaccgatggt ttcgacgatc gccgaatcgc
    12841 ccatgtcggg gttggcgtcg tcctgcgagt agccggggaa gaacaggcct acgggcattt
    12901 ccacgggtgc ggtgtaccag cgatcgccgg ttgcgctcac ccgcaggccg aagtcggtgc
    12961 cgttgcggca catcgcggtg acgatcgtcg acgcctcgat gccgcctgcc ggatccgtca
    13021 tcgacttgcc cattgccatc gcaatgttga ggaagaactg gtcgttcctg ccgatgaagc
    13081 ccaatgcgtc ggcgagcgcg tcgcggtcgg cgcaggcgcg ggcgagggcg ggcgcgattt
    13141 cgcgcaggaa cagcgcggag cacgcgacgt tgcgctggtg catctcgtcg cccatggcga
    13201 gcccgcgtgc aatgatcccc ttgagcggca ggccgccgcg cagcgtcagc gccgacgaaa
    13261 gcgcgggccc gagcacgtcg gcaatccagc gcaggcgcgc gagcacttcc ccgtcgttcc
    13321 cgccgaagcg catgaccttg ccgaggccct cgttgatcgt gcagtacgcg cgattgccga
    13381 acgcgccgtt ctcgacgatc atgaccggct ggctcgccgt ggtcattccg gtcatcgggc
    13441 cgacggcgcc gaagtgatga ttggggtgaa acgagaattc gccggccgcc gcggccttcg
    13501 ccgcgtcgtc gagcccggcg gcccagccct cgaacaccgc gatgccgcag acggcgccgc
    13561 gcatcgggcc gcacatccgg tcccaggtga tcggaggacc ggcgtggagg atcacgttcc
    13621 tgccgaggcc ctcgatgacg gcgtgcgccg gctggacgtc gacgagcacc ggctgtgccg
    13681 cgagaatccg gcgcagcgct tccctgttcg cgagctcgat tttctgcacg ttccgatctc
    13741 ctcgtcccga gggcttcccg ttctcgatgg ggtgtccgat gataccggac gcaatgcgcc
    13801 ccgggcaata aacatcgaat gaacgcgtca tcatcggtat agggtgtgaa tttcgaaaaa
    13861 gtggcgcagc gcgcgcgctt cgcgtaatct ttcgccactc gcgctgcttc gcgtcgcggc
    13921 aagcgaagat ccgtccatgc cgaaacagcg ggagccagga gccagacccg ggaatcgatc
    13981 gatgtcagca acaggccgga ttccgaaccg tctcgtgatc gccatcggcg gcaacgccac
    14041 gcatccggag ggcatccgcg gcacgccgtc cgagcaggtc gaagtggccg cgaagctcgg
    14101 cgacgcgttg ctgccggtga tggagctcgg caacgagttg atcatcacgc acgggaacgg
    14161 gccggtcgtc ggcaagatcc tgatgcgcca gtcgatcgcc aggcagcgcg tcgagccgat
    14221 gtcgctcgac atctgcgtcg cgcacagcca gggcggcatc gcctatctgt tgatgcaggc
    14281 gctcgagaat gcgttgcgcg cgaagggcaa cccgcgccat gtcgtctgcc tgctgacgca
    14341 ggtcgaggtc gacccgaacg accctgcatt tgccgatccc tcgaagccga tcggctattt
    14401 ctattcggag caggaagcgc gcgcgctcaa tgcggagctc gggtggcaga tgcgcgagga
    14461 ttcgggtcgc ggctggcgcc atgtcgtggc gtcgccgcac ccgaagcaca tcgtcgacat
    14521 ttcgctcgtg cagaccgtcg cgaagagcgg cgcggtcgtc atcgcgggcg gtggcggcgg
    14581 cgtgccggtc gtgcgcacgg gcaagggcca gcggcacggc gtcgaggcgg tcatcgacaa
    14641 ggaccgcaca tcggcactca tggccaacgt tctgcacatc gacgatctga tgatcctgac
    14701 cgccgtcccc cgcgtggcga tcaacttcgg caagcccgat cagcaggccc tcgatcgcgc
    14761 gtcgctgtcg caggtcagga agtggcacgc cgaaggacag tttccccccg gcagcatggg
    14821 accgaagatc gacgcggcaa tccagttcct gcaaggaggc ggtcgccgcg tgatgatcgg
    14881 gcggctcgag gatgccatgc ccatcctgcg cggcgatgca ggcacgcaca tcgttgccga
    14941 ctgatcgcct tcgtcggatc gaagccgtcg atcggcc
//
LOCUS       DASSVX010000324         3801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_447978, whole genome shotgun sequence.
ACCESSION   DASSVX010000324 DASSVX010000000
VERSION     DASSVX010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447978"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VFO53_13875"
     CDS             <1..1097
                     /locus_tag="VFO53_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8877414.1"
                     /translation="RGLTKRFGRQDALRDVSLAVEAGEFVALLGPSGCGKTTLLRMIA
                     GFDLPDAGRILLSGEDLATTPPELRPVRTVFQSYALFPHMSVEGNVAFPLRMARVPAG
                     ERKTRIEGALEDVRLTGFNKRYPHELSGGQKQRVAIARALVTHPTMLLLDEPLAALDA
                     KLREDLSIELINLQKEVGIAFVYVTHDQTEALALSHRIAVMNRGRIEQLDEPSKIYGY
                     PRTSFVADFIGHCNLFSGPVSQSNGSVAIVDVEGLGPVQVATQASVPGATRGTVALRP
                     EKIRIAASAAQESNAASQLGDPHANLYRGTVHDLLYMGDVTVYRVGTPGGTVVEALLA
                     NSQSGRAKFFEVGDAVEVSWPVAAGHFIAE"
     gene            1105..1998
                     /locus_tag="VFO53_13880"
     CDS             1105..1998
                     /locus_tag="VFO53_13880"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8877415.1"
                     /translation="MTARASTRLARWLVTGPPLLYLLVFFAAPTLIMVLASFRSPAEF
                     GGLAPLVDEAGKLDLNVDSYLRFVTEPVFMRVFAKSAGYATLTTLFCLLLAYPLATLI
                     AKSSRKYRDLLLLLVILPFWSNFLIRIYAWMIILGPNAALAKLVNATLALFGAQPVPL
                     LFSGFAVLVCLVYVHLPFMVLPLYANLEKHDQALLDAAQDLGANGWQRFWRITFPLSL
                     PGVYAGAALVFIPALGIFAIPDILGGPADTLIGNLIKQQFLETRDWPFGSVLSIVLTV
                     AALVLAGSAAWVARRGARRDA"
     gene            1991..2773
                     /locus_tag="VFO53_13885"
     CDS             1991..2773
                     /locus_tag="VFO53_13885"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8877416.1"
                     /translation="MRRRSPWLWAAAILAYAFLYVPLLIVVVYSFNDSRLNAEWVGFT
                     LGWYRTLAGDEQMLRAAANSLVIGIVASGVSTVLGTMAGLAMHRYRLRLLPILVFAPI
                     AIPEILTGVSLLIFFVLLNFTLGLVSVALAHIAFCIGFVAIVVRARLAGMDETLTEAA
                     RDCGASPAKAFRHVTLPLIMPGVIAGALMAFTLSIDDFVITFFTAGAGTVTLPLQIYS
                     MIKIAVTPEVNAVSTLLMLLTLLLIVAAAKLSPSLLRSQGET"
     gene            2818..>3801
                     /locus_tag="VFO53_13890"
     CDS             2818..>3801
                     /locus_tag="VFO53_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET8877417.1"
                     /translation="MFAAPAQARDELHLYNWNNYIAPETVKRFESVCQCDVVQTYYSD
                     NEELLAKLAAGATGYDVLVPTSNAVQALIRGGQLKPLDKTAIPNIRNIDPAYLNTPFD
                     PGNQYSVPYAMSTTILGYNDVKMKELGLPTDTWAVIFEPKYLEKVKGRVTVLDSANEL
                     FAAALKYLGYSANDVDPKHWDAAAAVIRKAKPYWAAFNASSYIKELTVGNIWLVHGYS
                     NDIFQANQDAQAAGRPFHILQGVPKEGAVLAVDNMVIHKAAPRPDLALRFINFMLEGR
                     NSAELTNLIGSGNPNLDAMKYIKPELTKLPAVFPPKELQAKLEQLKDLTVAQ"
BASE COUNT          610 a         1300 c         1204 g          687 t
ORIGIN      
        1 ttcgcggtct caccaagagg ttcggccggc aggacgcgct gcgcgacgtc tcgctcgcgg
       61 tcgaggcggg cgagttcgtc gcgctgctcg ggccgtcggg ctgcggcaag acgacgctgc
      121 tgcgaatgat cgcgggcttc gaccttcccg atgcgggtcg aatcctgctt tccggtgaag
      181 acctcgccac gacgccaccc gagctgcgtc ccgtgcgcac cgtgttccag agctatgcgt
      241 tgtttccgca catgagcgtc gaggggaacg tggccttccc gctgcggatg gcgcgcgtgc
      301 ctgccgggga gcgcaagacg cgcatcgagg gcgcgctcga ggacgtccgc ctcaccggct
      361 tcaacaagcg ctatccgcac gagctttccg gcgggcagaa gcagcgcgtt gccatcgctc
      421 gcgcgctcgt cacgcatccg acgatgctgc tgctcgacga gccgctcgcc gcactcgacg
      481 cgaagctgcg cgaggacctg tcgatcgagc tcatcaacct gcagaaggaa gtcggcatcg
      541 ccttcgtcta cgtcacgcac gaccagaccg aggcgctggc gctgtcgcac cggatcgccg
      601 tgatgaaccg cggacgcatc gagcagctcg acgagccgtc gaagatctac ggctatccgc
      661 gcacatcctt cgtcgccgac ttcatcggcc attgcaacct gttctcggga ccggtgagcc
      721 aaagcaacgg cagcgtcgcg atcgtcgacg tcgagggcct cggccccgtg caggtcgcga
      781 cgcaggcatc cgttcccggc gctacgcgcg gcacggtggc gctgcgtccc gaaaaaatcc
      841 ggatcgcggc gagcgcggca caagagagca acgccgcttc gcagcttggc gatccgcatg
      901 ccaacctcta tcgcggcacg gtgcacgacc tcttgtacat gggcgacgtc accgtctatc
      961 gcgtgggcac gccgggcggc acggtcgtcg aggcgctgct cgcgaactcg caaagcggcc
     1021 gtgcgaagtt cttcgaggtc ggcgacgcgg tcgaggtgag ctggcccgtc gcagccggcc
     1081 atttcatcgc cgagtgatgc gtcgatgacg gcccgcgcgt cgacacggct cgccaggtgg
     1141 ctggtgacgg gaccgccgct tctgtacctg ctcgtgttct tcgcggcgcc gacgctcatc
     1201 atggtgctcg cgtcgtttcg ctcgccggcc gaattcggcg gcctcgcgcc gctcgtcgac
     1261 gaggcgggca agctcgacct caacgtcgac agctacctgc ggttcgtcac cgagccggtg
     1321 ttcatgcgcg tgttcgcgaa gtcggcaggc tatgcaacgc tgacgacgct tttctgcctg
     1381 ctgctcgcct acccgctcgc gacgctgatc gcgaagagca gcaggaaata ccgcgacctg
     1441 ctgctgcttc tcgtcatcct gccgttctgg agcaacttcc tgatccgcat ttacgcgtgg
     1501 atgatcatcc tcggcccgaa cgcggcgctc gcgaagctcg tcaacgccac gctcgcgctg
     1561 ttcggcgcgc agccggtgcc gctgctgttc agcggcttcg ccgtgctcgt atgcctcgtc
     1621 tacgtgcacc tgcccttcat ggtgctgccg ctctatgcga acctcgagaa gcacgaccag
     1681 gcgctgctcg acgccgcaca ggacctcggc gccaacgggt ggcagcgctt ctggcgcatc
     1741 acgttcccgc tgtcgctgcc cggcgtgtac gccggagccg cgctggtgtt cattcccgcg
     1801 ctcggcatct tcgccattcc cgacattctc ggtgggcccg cggacacgct gatcggcaac
     1861 ctgatcaagc agcagttcct cgaaacgcgc gactggccct tcggcagcgt gctgtcgatc
     1921 gtgctgaccg tcgcggcact cgttctcgcc ggcagcgcgg cgtgggtcgc tcgccgggga
     1981 gcgcgccgcg atgcgtaggc gctcgccatg gctttgggcg gccgcaatcc ttgcctatgc
     2041 gttcctgtat gtgccgcttc tgatcgtcgt cgtctattcg ttcaacgatt cgcggctgaa
     2101 cgccgaatgg gtcggcttca cgctcggctg gtaccgcacg ctggcgggcg acgagcagat
     2161 gctgcgggcg gccgcgaact cgctcgtgat cggcatcgtc gcgagcggcg tgtcgacggt
     2221 cctcggcacg atggccgggc tcgcgatgca ccgttaccgg ctgcgcctgc tgccgatcct
     2281 ggtcttcgcg ccgatcgcca ttcccgagat cctgacgggc gtctcgctgc tgattttctt
     2341 cgtgctcctg aacttcacgc tgggcctcgt gtcggtcgca ctggcgcaca tcgcgttctg
     2401 catcggcttc gtggccatcg tcgtgcgcgc gcgcctcgcc ggaatggacg agacgctgac
     2461 cgaagcggcg cgcgactgcg gcgcatcgcc cgcgaaagcg ttccgccatg tgacgctgcc
     2521 gctgatcatg cccggcgtga tcgccggtgc gctgatggcg ttcacgctgt cgatcgacga
     2581 tttcgtcatc acgttcttca cggcaggcgc cggcaccgtc acgctgccac tgcagatcta
     2641 ctcgatgatc aagatcgccg tgacgcccga agtgaacgcc gtgtcgacgc tgctgatgct
     2701 gctcacgctg ctgctgatcg tcgctgctgc caagctgtcg ccaagcctgc ttcgctccca
     2761 aggggaaacc tgaccatgac ctcccgcttc cttgccgcgg cattcgcggc attcttcatg
     2821 tttgccgcac ccgcgcaggc gcgcgacgag ctgcacctct acaactggaa caactacatc
     2881 gcgccggaga ccgtcaagcg cttcgagagc gtttgccagt gcgacgtcgt gcagacctac
     2941 tactcggaca acgaggagct gctggccaag ctggccgcgg gtgccaccgg ctatgacgtg
     3001 ctggttccaa cgagcaatgc cgtgcaggcg ctgatccgcg gcgggcaatt gaagccgctc
     3061 gacaagaccg cgatcccgaa catcaggaac atcgatcccg cctatctgaa cacgccgttc
     3121 gatcccggca accagtactc ggtgccctac gcgatgtcga cgacgatcct cggctacaac
     3181 gacgtcaaga tgaaggagct cgggctgccc accgacacgt gggcggtgat cttcgagccg
     3241 aagtacctcg agaaggtgaa gggccgcgtg accgtgctcg acagtgccaa cgagctcttc
     3301 gccgcggcgc tcaaatacct cggctattcg gccaacgacg tcgacccgaa gcactgggac
     3361 gctgcggcgg cggtgatcag gaaggcgaag ccctactggg ccgcgttcaa tgcgtcgtcg
     3421 tacatcaagg agctgacggt cggcaacatc tggctcgtgc acggctactc gaacgacatt
     3481 ttccaggcga accaggacgc gcaggcggcc ggcaggccgt tccacatcct gcagggcgtg
     3541 ccgaaggaag gcgccgtgct tgccgtcgac aacatggtga ttcacaaggc ggcgccgcga
     3601 cccgacctcg cgctgcggtt catcaatttc atgctcgaag ggcgcaactc ggccgagctc
     3661 accaacctga tcgggtcggg caatccgaac ctggacgcga tgaagtacat caagcccgag
     3721 ctcacgaagc tccccgccgt gtttccgccg aaggagctgc aggcgaagct cgagcagctc
     3781 aaggacctga cggtcgcgca a
//
LOCUS       DASSVX010000325         9161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_914911, whole genome shotgun sequence.
ACCESSION   DASSVX010000325 DASSVX010000000
VERSION     DASSVX010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914911"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1971
                     /locus_tag="VFO53_13895"
     CDS             <1..1971
                     /locus_tag="VFO53_13895"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET8877418.1"
                     /translation="NEIDALDSGRILDWMRTGLSFAASASPGPGLVPVCRFYIPPTHG
                     DSHFLSASADECAAVRAKINTDPNFSGYIEETSAAFYVALPDATGACPDRTANVYRLW
                     NARADSNHRYTPDFEVRESMIAQGYVPEGYGPQGPAMCSMGTFFLGDTYVRVTAASPF
                     SPGCDLAPITGTVYPGSEVEPQLASDPRDGHLIGVWQQDRWSDGGARGLRTGYSFDNG
                     LTWSVTQAPFTRCSGGNAQNGGDYPRASDPWVSIGPDGIAYQVAIAFNGATFAPGSSS
                     AVLASRSTDGGRTWSDPATLILDAGAFFNDKESITADPVVARYAYATWDRLEPDGHGP
                     SYFSRTTDGGATWEEAKPIHDPGARNQTLNNQIVVSAATSGAGSPTLFDFFTEFDVGQ
                     NNVTTTHLALVRSFDRGDTWSPPIVVADLRPVGTYDPQDPAQTLRDGAGIASVASGPN
                     GVLAAVWQDSRFAGGARDGIAFSRSTDGGSTWSTPTQINAVPAAQALLPAVAVRFDGT
                     IGVLYYDMRNDTADAATLPVDAWLATSDDGGVTWNERHVSGPFDFDLAPTTEGGHFIG
                     DYQALLGAGNDFAALFARTSTGMTNRTDIFASVFRSIGLRQPKSEYRAREAAAFPATP
                     AWQAKLDESARRTLRQRLIGSAPVAPFIPENR"
     gene            1968..2624
                     /locus_tag="VFO53_13900"
     CDS             1968..2624
                     /locus_tag="VFO53_13900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HET8877419.1"
                     /translation="MTRTPRPPRNTTADAPGEDEARIAPSKKALDDCRRCELWEHATQ
                     GVPGEGPAGAKLMLVGEQPGDEEDVQGRPFVGPAGRLLRTLLDEAGVSAERVYLTNAV
                     KHFYFEPRGKRRIHKTPMQRHIAACHVWLEGELARERPGIIVTLGATALAAVYGNRIS
                     ITQARSRRLENQAGTPILATYHPSAILRAPDHDARAKLRATLVADLRRAARLADNARA
                     "
     gene            complement(2704..4878)
                     /locus_tag="VFO53_13905"
     CDS             complement(2704..4878)
                     /locus_tag="VFO53_13905"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HET8877420.1"
                     /translation="MHQTDAPAVGGKRRRKWTATSALLPLALFGWAAASEAGITAIQI
                     GSRAPAFGGTAFGSVGAYENLAGVAYGEVDPDDPLNAIITDIALAPRNARGMVEYSMD
                     FSIFKPVDPSKGNHTLLYDVVNRGRMLLPSLNIGSSGSNPGDGYLENAGYTLVFSGWE
                     GDISSGLRIHLPVATNNDGGAITGPVRGEYIFTSPTSTEDLSGPPTYEAIGTDNRGAT
                     MTRRVHQHDAKEVIPNDQWAFADCTSTPFPGEPSTTKVCLKDGFDTNHIYELVYTAKN
                     PTVAGIGFAATRDFISFLRNGNGALTGHGNGVGNGDGRGRCHNRGQGAAHNGCAQPLP
                     VPADQNPLGNSIANAIIYGSSQSGRWIRTFIQLGFNQDESLHQVVEGAMPHIASNRGA
                     FNVRFAQPTRLSGTQHTEAQYPGAESPQTWEVSDDSLSGVDASQLDRCRATHSCPKVI
                     ATVTDTEYWQSLMALNTTDALGTRDLPIPGNVRIFQLSSSQHGSGSPLASPTAPPAVP
                     ANCQLARNPNPYIPYQRALLADLHDWVVAGVEPPASMYSTIASGTLVPVETMHFPYAG
                     GAVNFTLESVANRKFYLDRGAQFNVEDITGVMAEPPIARSAYALLLPQVDADGNDVGG
                     LRGVNIQVPVGTYMGWNPRKAGFSEGDSCDLTGGYIPFFHTKAERDAAGDPRLSLAER
                     YPTRDDYVAKVTAAANALVAKRLMLPQDAQLAIGLAQSADVP"
     gene            complement(5118..7163)
                     /locus_tag="VFO53_13910"
     CDS             complement(5118..7163)
                     /locus_tag="VFO53_13910"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HET8877421.1"
                     /translation="MTPNYPHLLAPLDLGFVTLANRVLMGSMHTGLEESRDGFAKLAA
                     FYAARARGGVGLIVTGGIAPNFAGRVDPRASQLSFGWQVAKHRQVTAAVHAEGGRIAM
                     QILHAGRYAYHPLSVAPSALRSPITPFTPRALTKWGIERTLAAYVRCARLAQDAGYDG
                     IEIMGSEGYLINEFIARRTNRRDDAWGGAYDNRIRFAVETVRRTRAAVGRDFIVIYRL
                     SMLDLVDDGSTWAEVVTLAKAIEAAGATLINTGIGWHEARIPTIATMVPRGAFTWVTA
                     RLRGAVSVPLIATNRINDPGVAEAILARGDADMVSMARPFLADADFVHKAAEGRADSI
                     NTCIACNQACLDHIFERKVATCLVNPYACRETQLVLEPARAQKRIAVVGAGPAGLACA
                     TTAAERGHRVTLFEASSVIGGQFNLARRIPGKEEFAETLRYFDRRLAEARVERRLGAP
                     AGVADLRHYDRVILATGVVPRAPGIAGVDHPRVASYVDIIEGRRIAGASVAIIGAGGI
                     GFDVAEFLTHTDDDDAHASTGSAGVRSLDAFRDEWGIDATYAAPGGIREPRQPAMRRR
                     VWLLQRKSTKVGEDLAKTTGWIRRTLLRRRSVSMLAGVVYERIDDAGLRILLDGVPTT
                     LGVDTIVICAGQEPRRDLAIALEEAGIAFSLIGGADVARELDAKRAIDQATRLALAL"
     gene            complement(7160..7756)
                     /locus_tag="VFO53_13915"
     CDS             complement(7160..7756)
                     /locus_tag="VFO53_13915"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HET8877422.1"
                     /translation="MHTVVATADLFDAPRLPPGLDYRPDFLDPGREASLLETFASLPF
                     REAPFRQYTARRRVVRFGSADDAAASELQHGGPLPAWLAALRRDAAAVADVDAESFVH
                     ALVTEYRPGTPIGWHRDAPDYGTVVGISLAGACRMRFRPYARQLDRAAVIAVELAPRS
                     LYVMRGDVRWRWQHQIPPVKALRYSVTFRTLRSPGSQP"
     gene            complement(7788..>9161)
                     /locus_tag="VFO53_13920"
     CDS             complement(7788..>9161)
                     /locus_tag="VFO53_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8877423.1"
                     /translation="DEPTNHLDIDGIALLEAQVQGARAAIIVTHDRVFLDHVCTRIIE
                     LDRGLVRSYPGNFAAYQARKASELAAESVSARKFDQFWRQEEAWIRKGVEARRTRDEG
                     RVQRLEALRRERALRRERMGNVRLSLDAGERSGRLVAELSHVSKRFGERDIVRDLSLR
                     IMRGDRLALLGRNGAGKSTLLRIILGEIAPDAGKVRLGTRLAVAYFDQLRAALDPQRT
                     LVQTISPGSEWVETPAGRKHVLSYLADFLFPPERANAPVATLSGGERNRLLLARLFAQ
                     PANLLVLDEPTNDLDIESLELLEATLASYEGTLLVVSHDRAFLDNVATQTLVAEGDGR
                     WVEYAGGYSDWLVQRAPAALATAAPARKAKAPRERAADGRVRLTFNEQRELDALPAEL
                     EALEREQHALSARMCAADYHRQDVDAIKADRRRAEEIERLLAERFARWGELDAKAEAA
                     AQRAQPR"
BASE COUNT         1403 a         3126 c         3155 g         1477 t
ORIGIN      
        1 aacgagatcg acgcgctcga ctcgggccgg atcctcgact ggatgcgcac cgggctttcg
       61 ttcgccgcgt cggcgtcgcc gggcccgggc ctcgttccgg tgtgccgctt ctacattccc
      121 cccacgcacg gcgattcgca cttcctgtcc gcgtcagccg atgaatgcgc ggcggtccgc
      181 gcgaagatca ataccgatcc gaacttcagc ggctacatcg aggagacctc ggccgcgttc
      241 tacgtggcgc tgcccgacgc gacgggtgcg tgccccgacc gcaccgcgaa cgtataccgg
      301 ctgtggaatg cgcgcgccga ctcgaaccac cgctacaccc ccgacttcga ggtgcgcgag
      361 tcgatgatcg cgcagggcta cgttcccgaa ggctacggcc cgcagggccc ggcgatgtgc
      421 tcgatgggca ctttcttcct cggcgacacg tacgtgcgcg tgacggcggc gtcgcccttc
      481 tcgccgggtt gcgacctcgc gccgatcacg ggaaccgtct atccgggcag cgaggtcgag
      541 ccgcagctcg caagcgatcc gcgcgatggc cacctgattg gcgtttggca acaggatcgc
      601 tggtcggacg gcggcgcgcg tggccttcgc accggctatt cgttcgacaa cggcctcacg
      661 tggtcggtga cgcaggcgcc attcacccgc tgcagcggcg gcaatgcgca gaacggcggt
      721 gattacccgc gcgcgagcga tccatgggtg tcgatcggcc ccgatggcat cgcctaccag
      781 gtcgccatag cgttcaatgg cgccacgttc gcgcccggtt cgtcgagcgc cgtgctcgcg
      841 agccgctcga cggacggcgg acggacgtgg agcgatccgg cgacgctgat cctcgatgcc
      901 ggcgcgtttt tcaacgacaa ggaatcgatc accgccgatc cggtggttgc ccgctacgcc
      961 tatgcgacgt gggaccggct cgagccggac gggcatgggc cgtcgtattt ctcgcgtacg
     1021 acggatggcg gcgcgacgtg ggaggaggcg aagccgatcc acgacccggg tgctcgtaac
     1081 cagacgctca ataaccagat cgtcgtgtcg gcggcaacga gcggggccgg ttccccgacg
     1141 ctgttcgatt tcttcaccga gttcgacgtc ggacagaaca acgtcacgac aacgcatctc
     1201 gcgctcgtgc gctcgttcga tcgcggcgac acgtggtcgc cgccgatcgt cgttgccgat
     1261 ctgcggccgg tgggcaccta cgatccgcag gatccggcgc aaacgctgcg cgatggcgca
     1321 ggcatcgcat cggttgccag cgggccgaac ggcgtgctgg ccgccgtctg gcaggactcg
     1381 cggttcgcag gcggcgcgcg cgacggcatt gccttttcgc ggtcgaccga tggcggaagc
     1441 acatggagca cgccaacgca gatcaacgcc gttccggccg cgcaggcgct gctgcccgcc
     1501 gtcgctgtcc ggttcgacgg cacgatcggc gtgctgtact acgacatgcg caacgatacg
     1561 gccgacgccg ccacgctgcc cgtcgacgcg tggcttgcga cgtccgacga tggcggcgtc
     1621 acctggaacg agcgccacgt cagcggaccg ttcgatttcg acctggcgcc gaccaccgag
     1681 ggcggccact tcatcggcga ctaccaggca ctgctgggtg cgggcaacga ttttgccgcc
     1741 ctgttcgcgc gcaccagcac cggcatgacc aaccgcacgg acattttcgc gtcggtcttc
     1801 cgctcgatcg gcctgcggca gccgaaatcc gaataccggg cgcgcgaggc agcggcattc
     1861 ccggcgacac cggcctggca ggcgaagctc gacgaaagtg cgcgccggac gctgcgccag
     1921 cggctgatcg gctccgcgcc cgtggcaccc ttcattcccg aaaatcgatg acgcgcacgc
     1981 cgcggccgcc gcgaaacacg actgcggacg ccccgggtga agacgaggct cgcattgcgc
     2041 cgtcgaagaa agcgctcgac gattgccggc gctgcgagct gtgggagcac gcaacccaag
     2101 gcgtgcccgg cgaaggaccc gccggcgcca agctgatgct tgtcggcgaa cagccgggcg
     2161 acgaagagga cgtgcagggc cggccgttcg tcggccctgc aggacggctg ctgcgcacgc
     2221 tgctcgacga ggccggcgtt tctgccgagc gtgtgtatct caccaatgcc gtcaagcact
     2281 tctatttcga gccgcgcggc aagcggcgca tccacaagac gccgatgcag cggcacatag
     2341 ccgcgtgtca cgtctggctc gaaggcgagc ttgcgcgcga gcgcccgggc atcatcgtga
     2401 cgctcggcgc aacggcgctc gctgccgtgt acggaaaccg aatctcgatc acgcaggcgc
     2461 gcagccgcag gctcgagaat caggccggca cgccgatcct cgccacctat cacccgtcgg
     2521 cgatcctgcg cgcgcccgac cacgacgcac gcgcgaagct tcgcgcgacg ctcgtcgccg
     2581 atcttcggcg cgcggcacgg ctcgcggaca acgcccgagc atgacatccg gggcatcggc
     2641 cgatgccccg gatgcaactt cactcgtgcc gtgcgatgcg ctgcacgctt gttgcgctgc
     2701 tactcatgga acatcggccg actgcgcgag gccgatcgcg agctgcgcgt cctgcggaag
     2761 catcagccgt ttcgcgacca gcgcgttcgc cgcggcggtc accttcgcaa cgtagtcgtc
     2821 gcgcgtcgga tagcgctcgg cgagcgagag gcgcggatcg ccggccgcgt cgcgctcggc
     2881 cttcgtatgg aagaacggga tgtagccgcc ggtcagatcg cacgagtcgc cttcgctgaa
     2941 tccggccttg cgcgggttcc agcccatgta cgttccgacc gggacctgga tgttgacgcc
     3001 gcgcaggccg ccgacatcgt tgccgtcggc gtccacctgc ggcagcagca gcgcataggc
     3061 gctgcgggca atcggcggtt cggccattac gccggtgatg tcctccacgt tgaactgcgc
     3121 gccgcgatcg aggtagaact tgcggttggc cacgctttcc agcgtgaagt tcaccgcgcc
     3181 gcccgcatac gggaaatgca tcgtctcgac cggcacgagc gttcccgagg cgatcgtcga
     3241 gtacatgctc gctggaggct cgaccccggc gacgacccag tcatgcaggt ccgcaagcaa
     3301 tgcgcgctga tacgggatgt acgggttggg attgcgcgcg agctggcaat tcgccgggac
     3361 ggcgggcggc gccgtcggcg atgcgagcgg gcttcccgag ccatgctgcg agctcgacag
     3421 ctggaagatg cgcacgttgc cgggaatggg cagatcgcgc gtgccgaggg cgtcggtggt
     3481 gttgagtgcc atcagcgact gccagtactc ggtatcggtg accgtcgcga tgaccttcgg
     3541 acagctgtgc gtcgccctgc agcgatcgag ctggctcgcg tcgacgccgg acagcgaatc
     3601 gtcgctgacc tcccacgtct gcggtgactc ggcacccggg tactgcgcct cggtgtgctg
     3661 cgtgcccgac aggcgcgtgg gctgcgcgaa gcgcacgttg aacgcgccgc ggttcgacgc
     3721 gatatgcggc atcgcgccct cgaccacctg gtgcaggctt tcgtcctggt tgaagccgag
     3781 ctggatgaac gtgcgaatcc agcggccgct ttgcgaggag ccgtagatga tcgcgttggc
     3841 gatcgagttg cccagcggat tctggtcggc tggaacgggc agcggctgcg cgcagccatt
     3901 gtgcgcggcg ccctgcccgc gattgtggca acgcccgcgc ccgtcgccat tgccgacgcc
     3961 gttgccatgc ccggtcagcg cgccgttgcc gttgcgcagg aaagagatga aatcacgcgt
     4021 ggcggcaaag ccgataccgg cgaccgttgg gttcttcgcc gtgtagacga gttcgtagat
     4081 gtgattggtg tcgaagccgt ccttgaggca caccttcgtc gtgctgggtt cgcccgggaa
     4141 cggcgtgctc gtgcaatcgg cgaatgccca ctggtcgttc ggaatcactt ccttcgcgtc
     4201 gtgctgatgc acgcgccgcg tcatcgtcgc gccgcggttg tcggtgccga tcgcttcgta
     4261 ggtcgggggg ccggacagat cctcggtcga ggtgggcgag gtgaagatgt attcgccccg
     4321 taccgggccg gtgatcgcgc cgccgtcgtt gttcgtagcg accggcagat ggatccgcag
     4381 tccgctgctg atgtcgcctt cccagccgct gaacacgagc gtgtagcccg cattctcgag
     4441 gtagccgtca ccgggattgc ttccactgct gccgatgttg agacttggca gcaacatgcg
     4501 gccacggttc accacgtcgt agagcagcgt gtggttcccc ttcgacgggt cgaccggctt
     4561 gaagatcgag aagtccatcg agtactcgac catgccgcgc gcgttgcgcg gcgcgagcgc
     4621 gatgtcggtg atgatggcgt tcagcggatc gtccgggtcg acttcgccgt atgcgacgcc
     4681 ggcgaggttc tcgtacgccc cgacgcttcc gaatgccgtg ccgccgaagg ctggtgcgcg
     4741 gctgccgatc tggatcgccg tgatccctgc ctcggatgcc gccgcccatc cgaacagcgc
     4801 caatgggagc aatgcgctcg tcgcagtcca cttgcgccgc cgcttgccgc ccaccgccgg
     4861 tgcgtcggtc tgatgcaacg gggaagaccg tgccagatac gccatcattc gactcctcct
     4921 aacgtgctgc gtggattgcg ggcatcgccg atgcccgacc ttccctcccc gttccgtcgt
     4981 tgcacttcgt ccccgcggca ctttcaattg cggtgcggag cggcggtgac gcatgtcacc
     5041 ctgtgcaatc gttcgcgcaa cttatccgcg gcgcggggtg aagtcaatgc ggcgcgggcg
     5101 ggtccgtaac ggagggctta cagcgccagg gcgagacgcg tcgcctggtc gatcgcgcgt
     5161 ttcgcgtcga gctcgcgcgc gacgtcggcg ccgccgatca gcgagaacgc aatgccggct
     5221 tcttcgagcg caatcgcgag atcgcgtcgc ggctcctgtc cggcgcagat cacgatcgtg
     5281 tcgacgccga gcgtcgtcgg cacgccgtcg agcaggatgc gcaggcccgc gtcgtcgatg
     5341 cgctcgtaca cgacgccggc aagcatcgag acgctgcgcc ggcgcagcag cgtgcgccga
     5401 atccagccgg tcgtcttcgc aagatcctcg ccgaccttgg tcgatttcct ctgcaatagc
     5461 cagacgcgcc ggcgcatcgc gggctggcgc ggttcgcgaa tgccgccggg cgccgcatac
     5521 gtggcatcga tcccccattc gtcgcgaaat gcgtcgaggc tgcgcacgcc tgcggaaccg
     5581 gtgctcgcgt gcgcatcgtc gtcgtccgtg tgcgtcagga attcggcgac gtcgaagccg
     5641 atgccgcctg cgccgatgat cgcgacgctt gcacccgcga tacggcggcc ctcgatgatg
     5701 tcgacgtagc tcgcgacgcg cggatggtcg acgcccgcaa tgccgggggc gcgcggaacc
     5761 acgccggtcg cgaggatcac gcggtcgtag tggcgaaggt cggcgacgcc cgcaggcgcg
     5821 ccgagccgcc gctcgaccct cgcttccgcg agacgccggt cgaaatagcg gagcgtttcg
     5881 gcgaattcct ccttgccggg aatgcgccgg gcgagattga actggccgcc gatgacgctg
     5941 ctcgcttcga acagcgtcac gcggtgcccc cgttcggcgg ccgtcgtcgc gcacgcgagg
     6001 cccgccgggc ccgcaccgac cacggcgatg cgcttctgcg cgcgcgccgg ctcgagcacg
     6061 agctgcgtct cgcggcaggc gtacggattg acgaggcagg tggcgacctt gcgctcgaag
     6121 atatggtcga ggcacgcctg gttgcaggcg atgcacgtgt tgatgctgtc ggcgcgtcct
     6181 tccgcggcct tgtgcacgaa gtcggcgtcg gcgaggaacg ggcgcgccat cgacaccatg
     6241 tcggcgtcgc cgcgggcgag gatcgcttcg gccacgccgg gatcgttgat ccggttcgtc
     6301 gcgatgagcg gaacgctgac ggcgccgcgc agccgcgcgg tcacccaggt gaacgcgccc
     6361 cgcggcacca tcgttgcgat cgtcggaatg cgcgcctcgt gccagccgat acccgtgttg
     6421 atcagcgtcg cgccggcggc ttcgattgcc ttcgcgagcg ttaccacttc ggcccacgtg
     6481 ctgccgtcgt cgacgaggtc gagcatcgac aaccggtaga tgacgatgaa gtcgcgaccg
     6541 acggctgcac gcgtgcggcg aaccgtttcg acggcgaagc ggatgcgatt gtcgtacgcg
     6601 ccgccccacg cgtcgtcgcg ccggttggtc cggcgtgcga tgaactcgtt gatcaggtag
     6661 ccttccgagc ccatgatctc gatgccgtcg tagcccgcgt cctgcgcgag gcgggcgcag
     6721 cgcacatagg ctgcgagcgt gcgctcgatg ccccacttcg tgagtgcgcg cggcgtgaat
     6781 ggcgtgatcg gcgagcgcag cgccgatggc gcgaccgaca gcgggtgata tgcatagcgc
     6841 cccgcatgca gaatctgcat cgcgatgcgg ccgccttcgg cgtgcacggc cgcggtcacc
     6901 tgccggtgct tcgccacctg ccagccgaac gacagttgcg aggcgcgcgg atcgacgcgc
     6961 ccggcaaaat tcggcgcaat gccaccggtg acgatgaggc cgacgccgcc acgtgcgcgc
     7021 gccgcgtaga acgcagcgag cttcgcgaag ccgtcgcgcg actcctcgag tcccgtgtgc
     7081 atcgagccca tcagcacgcg gttcgcgagc gtgacgaagc cgaggtcgag cggggcgagg
     7141 agatgggggt agtttggggt cagggctgtg atcccggact gcgcagcgtg cggaacgtca
     7201 ccgaatagcg cagcgccttg accggcggaa tctgatgctg ccagcgccag cggacgtcgc
     7261 cgcgcatcac gtagagcgag cgcggcgcga gctcgaccgc gatgaccgca gcgcggtcga
     7321 gctgccgcgc gtacggacga aagcgcatcc tgcacgcgcc cgcgagcgag atgccgacca
     7381 cggttccgta atccggcgcg tcgcgatgcc acccgatcgg cgtgcccggc cggtattcgg
     7441 tgacgagcgc atggacgaag gattcggcgt cgacgtccgc aacggcggcc gcgtcgcgac
     7501 gcagtgccgc gagccatgca ggcagcggcc cgccgtgctg cagctcgctc gccgcggcat
     7561 cgtcggcgct tccgaagcgg accacccgcc ggcgcgccgt atattgccgg aacggcgcct
     7621 cgcgaaatgg cagcgacgcg aatgtctcga gcagcgaggc ctcgcggccg ggatcgagga
     7681 aatccgggcg atagtcgagc ccgggcggca gccgcggagc gtcgaaaaga tcggcagtgg
     7741 cgacaaccgt gtgcatcggc ggcagatggg tgcgacgacg ggcctcttca acgcggttgt
     7801 gcgcgctgcg ccgccgcttc ggccttcgcg tcgagctcgc cccagcgcgc gaagcgctcg
     7861 gcgagcagcc gctcgatctc ctcggcgcgg cggcgatcgg ccttgatcgc gtcgacgtcc
     7921 tgccggtgat agtcggccgc gcacatgcgc gcgctcagcg catgctgctc gcgctcgagt
     7981 gcttcgagct cggcgggcag cgcatcgagc tcgcgctgct cgttgaacgt cagtcgcacg
     8041 cggccgtcgg ccgcgcgctc gcgcggcgct ttcgcctttc tcgccggcgc cgcggttgcg
     8101 agagccgccg gcgcgcgctg caccagccag tcggaatagc cgcccgcgta ttcgacccag
     8161 cggccgtcgc cttcggcgac gagcgtctgc gttgcgacgt tgtcgagaaa ggcgcgatca
     8221 tggctcacga cgagcagcgt gccttcgtac gacgcaagcg ttgcctcgag cagctcgagc
     8281 gattcgatgt cgaggtcgtt cgtcggctcg tcgagcacca gcaggttcgc cggctgcgcg
     8341 aaaagccgcg cgagcagcag ccggttgcgc tcgccgccag agagcgtcgc aaccggcgca
     8401 ttcgcgcgct cgggcggaaa caggaaatcg gcgagatacg acagcacgtg cttgcgcccg
     8461 gccggcgttt ccacccattc gctgccgggg ctgatcgtct gcaccagggt gcgctgcgga
     8521 tcgagtgccg cgcgcaactg gtcgaagtac gcgacggcga gccgcgtgcc gaggcgcacc
     8581 ttccccgcgt cgggcgcgat ctcgccgaga atgatccgca gcagcgtcga cttgccggcg
     8641 ccgtttcgcc cgagcagcgc caaccggtcg ccgcgcatga tccgcagcga aagatcgcgc
     8701 acgatgtcgc gttccccgaa gcgcttcgat acgtgcgaga gctcggcgac gagcctcccc
     8761 gagcgctcgc cggcgtcgag cgaaagccgc acgttgccca tgcgctcgcg acgaagcgca
     8821 cgctcgcgac gcaacgcctc cagccgctgc acccggcctt cgtcgcgcgt gcgccgcgcc
     8881 tcgacgccct tgcgtatcca cgcctcctcc tggcgccaga actggtcgaa cttgcgtgcc
     8941 gaaacgctct cggcggcgag ctcggacgcc ttgcgcgcct ggtacgccgc gaaattgcca
     9001 gggtacgaac gaaccagtcc gcggtcgagc tcgatgattc gcgtgcagac atgatcgagg
     9061 aagacacggt catgggtgac gatgatcgcc gcacgcgcgc cctgcacctg cgcttcgagc
     9121 agcgcgatgc cgtcgatgtc gagatggttg gtgggctcgt c
//
LOCUS       DASSVX010000326         3775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1398115, whole genome shotgun sequence.
ACCESSION   DASSVX010000326 DASSVX010000000
VERSION     DASSVX010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398115"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..112
                     /locus_tag="VFO53_13925"
     tRNA            38..112
                     /locus_tag="VFO53_13925"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:70..72,aa:Val,seq:cac)
     gene            175..861
                     /locus_tag="VFO53_13930"
     CDS             175..861
                     /locus_tag="VFO53_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET8877424.1"
                     /translation="MPSAPRAFVFDAYGTLYDVHSVVTLAEQLAPRQGAVLSQVWRTK
                     QLEYTWLSSLMRSPSYAREDFSTLTARALDYAVAQLMVPLDAEQRQRLLDAYRTLAPF
                     PDAADALAQLAPRPRWILSNGTLGMLTPLVASSSLALHIDAILSVDAAGIYKPAPAVY
                     QLAVDRLRLPADEIAFVSSNGWDAAGAKVFGFTTFWINRYGVPVERHAPEPDFIVGSL
                     AHIAAIAAEK"
     gene            complement(964..1869)
                     /locus_tag="VFO53_13935"
     CDS             complement(964..1869)
                     /locus_tag="VFO53_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HET8877425.1"
                     /translation="MTLNELRYIVAVARERSFGRAAAKCFVSQPALSVAIQKLEDELG
                     APLFERGKTEITITPIGERIVEQAQKVLEEAARIREIAIGGRNQLVGTLRLGIIHTVA
                     PYLLPDLITELHGAAPQMPLDIEETLTENLEAGLRTGRVDAAIIALPFDPPGVVTELM
                     YEEPFQVVVPQGHKWARRRSVAPDELAGEHTILLNVGHCFRNQVLDACPELNQSDAQV
                     TRTNSLETVRNMVASGLGVSVLPRDALTPRYHNQLVVPVPFTQPVPSRRIALAYRRSF
                     PRPAAIAAIRESVARCRAKAPARKK"
     gene            complement(1866..2744)
                     /gene="galU"
                     /locus_tag="VFO53_13940"
     CDS             complement(1866..2744)
                     /gene="galU"
                     /locus_tag="VFO53_13940"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET8877426.1"
                     /translation="MKKITKAVFPVAGLGTRFLPVTKASPKEMLPVVDKPLIQYAVEE
                     AAAAGITDMIFITGRNKRAIEDHFDKAYELETELLLRHKESLLEQVQAATPPGINCVY
                     IRQAEALGLGHAVLCAEPVVVDEPFAVLLADDLIHASTPVMAQMVELAGRENASVIGV
                     MPVAAEATASYGIVESEPEPVGGGVSRITRMIEKPKPGTTSSTLAVVGRYVLSPRIFH
                     HLRTMPAGAGGEIQLTDGIARLLGEERVLAYEFEGRRYDCGSKLGYLEATVDYALRHP
                     EVGDAFAQYLNRRSSQ"
     gene            complement(2741..>3775)
                     /gene="ligA"
                     /locus_tag="VFO53_13945"
     CDS             complement(2741..>3775)
                     /gene="ligA"
                     /locus_tag="VFO53_13945"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET8877427.1"
                     /translation="GRTGAVTPIARLEPVFVGGVTVTNATLHNEDEVRRKDVRIGDTV
                     VVRRAGDVIPEIVRVVIEARPADAREFVMPVTCPECGSAVERLPGEAIARCTGGLVCP
                     AQRKQALRHFASRHAMDIEGLGEKLIDQLVETGRVRTPADLYALRAEELATLERMADK
                     SAQNVIAAIDKSRATTLARFIYALGIRHVGEATARDLAAHFGALDPILAASEDALLDV
                     RDIGQILAESIRDFLAEPHNRTVIDALRKAGVRFPEGAPQRAAAGPLAGKSFVLTGTL
                     PTLARDEAKSMLEAAGASVIGSVSKKTDYVVAGADAGGKLVKAQALGVAIIDEAKMRE
                     MISNERGVAR"
BASE COUNT          583 a         1324 c         1207 g          661 t
ORIGIN      
        1 gcctatggtt aaatcgcggc tcctgtctgg gttcgcgggg cggttagctc agcggtagag
       61 cactgccttc acacggcagg ggtcactggt tcgaacccag tatcgcccac cacatgaacc
      121 aaggggttac gcgcgagtgt aacccctctt tttttgtcgg gacgctttta cacgatgcct
      181 tccgcgccac gcgcttttgt cttcgatgcc tacggcacgt tgtacgacgt gcactcggtg
      241 gtcacgcttg ccgagcaact cgcgccgcgc caaggcgcgg tgctgtcgca ggtgtggcgc
      301 acgaagcagc tcgagtacac gtggctgtcg tcgctgatgc gctcgccgag ctacgcgcgc
      361 gaagatttct cgacgctcac cgcgcgcgca ctcgattacg cggtcgcgca attgatggtc
      421 ccgctggatg ccgagcagcg gcagcggctg ctcgacgcgt accggacgct tgcgccgttc
      481 cccgacgccg ccgacgcgct cgcgcagctc gcccctcgcc cgcgctggat cctgtccaac
      541 ggcacgctcg ggatgctcac gccgctcgtg gcgtcgtcgt cgctcgcttt gcacatcgac
      601 gccatcctgt ccgtggatgc cgccggcatc tacaagccgg cacctgcggt ctaccagctc
      661 gccgtcgacc gcctgcggct gccggccgac gagatcgcgt tcgtctcatc caacggctgg
      721 gacgccgcag gggcgaaggt gttcggcttc acgacgttct ggatcaaccg ctacggggtc
      781 ccggtcgagc ggcacgcgcc ggaacccgac ttcatcgtcg gctcgctcgc gcatatcgcg
      841 gcgatcgccg ccgaaaagta gggtcagaga cgactttcat aaccgtcgaa aagtagggtc
      901 agagacgact ttcataagcg tcgaatcgca atctcgctga tgcggcgaaa gtcgtctctg
      961 accctacttt tttcgcgccg gcgctttcgc ccggcagcgc gcgacgctct cgcggatcgc
     1021 cgcgattgcc gccggacgcg gaaagctgcg ccgatacgcg agcgcgatcc gccgcgaggg
     1081 caccggctgc gtgaacggca ccggcacgac gagctggttg tgataacggg gcgtcaacgc
     1141 atcgcgcggc aacaccgaca cgccgagccc cgacgccacc atgttgcgca ccgtctcgag
     1201 cgaattcgtg cgcgtcacct gggcgtccga ctggttgagc tccggacagg cgtcgagcac
     1261 ctggttgcga aagcaatggc cgacgttgag caggatcgtg tgctctcccg ccagctcgtc
     1321 gggggccacc gagcggcgtc gcgcccattt gtgcccctgc ggaacgacga cctggaacgg
     1381 ctcctcgtac atcaactcgg tcacgacacc tggcggatcg aacggcagcg cgatgatcgc
     1441 cgcgtcgacc cggccggtgc gcaagcccgc ctcgaggttc tcggtcagcg tctcctcgat
     1501 gtcgagcggc atctgcggcg cggcgccgtg cagctccgtg atgagatcgg gcagaaggta
     1561 gggggcgacc gtgtggatga tgcccagccg cagcgtgccg accagctggt tgcgcccgcc
     1621 gatcgcgatc tcgcggatgc gcgcggcctc ctccagcacc ttctgcgcct gctcgacgat
     1681 ccgctcgccg atgggcgtga tggtgatctc ggtcttgccg cgctcgaaca gcggcgcgcc
     1741 gagctcgtcc tcgagcttct ggatcgccac cgacagcgcc ggctggctca cgaagcattt
     1801 cgccgcggcg cgaccgaagc tgcgctcccg tgcgaccgcg acgatgtagc gcagctcgtt
     1861 caaggtcact ggctggaccg tcggttcaaa tactgcgcga acgcgtcacc gacctcggga
     1921 tggcgcagcg cgtaatcgac cgtcgcctcg aggtagccca gcttgctgcc gcagtcgtag
     1981 cggcggccct cgaattcgta cgcgagcacg cgttcctcgc cgagcaatcg tgcaatcccg
     2041 tcggtgagct ggatttcgcc accggcgccg gccggcatcg tgcgcagatg atgaaaaatg
     2101 cgtggcgaca gcacgtagcg gccgaccacg gcgagcgtcg agctcgtcgt tcccggcttc
     2161 ggcttttcga tcattcgcgt gatgcggctg acgccgccgc cgaccggctc gggttcggat
     2221 tcgacgatgc cgtagctcgc ggtcgcctcc gccgcgaccg gcatcacgcc gatcaccgac
     2281 gcgttctcac gcccggcaag ctcgaccatc tgcgccatga ccggcgtcga cgcatggatc
     2341 aggtcgtcgg cgagcagcac cgcgaaaggc tcgtcgacga cgaccggttc cgcgcaaagg
     2401 accgcatgcc cgagcccgag cgcttcggcc tggcgaatgt agacgcagtt gattccgggc
     2461 ggcgtcgccg cctgcacctg ctcgagcaac gactccttgt gccgcagcaa aagctcggtt
     2521 tcgagctcgt aggccttgtc gaaatgatcc tcgatcgccc gcttgttgcg cccggtgatg
     2581 aagatcatgt cggtgatgcc ggccgccgcc gcctcctcca ccgcatattg aatgagcggc
     2641 ttgtcgacga ccggcagcat ctccttcggg ctcgccttcg tcaccgggag aaaccgggtg
     2701 ccgagcccgg ccaccggaaa aacggccttc gtgatcttct tcatctcgcg acaccccgtt
     2761 cgttcgaaat catttcgcgc atcttcgcct cgtcgatgat cgcgacgccg agcgcttgcg
     2821 ccttgacgag cttgccgccg gcgtcggcgc cggcgacgac gtaatcggtt ttcttcgaca
     2881 cgctgccgat gacgctcgcg cccgccgctt cgagcatcga cttcgcctcg tcgcgcgcaa
     2941 gcgtcggcaa cgtgccggtc agcacgaagc tctttccggc gagcgggccc gccgccgcgc
     3001 gttgcggcgc accctcggga aaacgcacgc ccgccttgcg cagcgcgtcg attaccgtgc
     3061 gattgtgcgg ctcggcgagg aaatcgcgaa tcgattccgc caggatctgt ccgatatcgc
     3121 gcacgtcgag aagcgcgtcc tcgcttgccg caaggatcgg atcgagcgcg ccgaaatgcg
     3181 cggcgaggtc gcgcgcggtt gcttcgccga catgccggat gccgagcgcg taaatgaagc
     3241 gcgcgagcgt ggtcgcgcgg cttttgtcga tcgcggcgat cacgttctgc gcgctcttgt
     3301 ccgccatccg ctcgagcgta gcgagctctt ccgccctcaa cgcataaagg tccgccggcg
     3361 tccgcacgcg gcccgtctcg acgagctggt cgatcagctt ctcgccgagc ccttcgatgt
     3421 ccattgcatg gcggctcgcg aaatggcgca gcgcctgctt gcgctgcgcc gggcagacga
     3481 gcccgccggt gcagcgcgca atggcctcgc cgggcagccg ctccaccgcg gaaccgcatt
     3541 cgggacacgt caccggcatc acgaattcgc gcgcatcggc cggccgcgcc tcgatcacca
     3601 cgcgaacgat ctccggaatg acatcgcccg cacggcgcac gacgacggtg tcgccgatgc
     3661 gcacgtcctt gcgccggacc tcgtcctcgt tgtgcagtgt cgcgttcgtc accgtgacgc
     3721 cgccgacgaa gaccggctcc agccgggcga tcggcgtgac ggcgccggtg cgccc
//
LOCUS       DASSVX010000327        12639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_281770, whole genome shotgun sequence.
ACCESSION   DASSVX010000327 DASSVX010000000
VERSION     DASSVX010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12639
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281770"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            455..967
                     /locus_tag="VFO53_13950"
     CDS             455..967
                     /locus_tag="VFO53_13950"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET8877428.1"
                     /translation="MRIERQGDTMVITAHARLVADPVTAWAVLTDYARYRDFIPGVRA
                     SRVVERRGSAVVVEQSDEFALSFLRMPVRVMYEIDEFPRARLQSRARASGLPPLSSTF
                     VLERTEAGVTLEYAGYIGPGLPLLGRLEQPALQRAAIRDFEALVDEIERRAATRSTRP
                     PHAAPARVPD"
     gene            1084..1239
                     /locus_tag="VFO53_13955"
     CDS             1084..1239
                     /locus_tag="VFO53_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HET8877429.1"
                     /translation="MNITLSAGPLISLIAGILILIIPRLLNYIVAIYLILIGLIGLFG
                     IGGLHVR"
     gene            complement(1265..1756)
                     /locus_tag="VFO53_13960"
     CDS             complement(1265..1756)
                     /locus_tag="VFO53_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877430.1"
                     /translation="MLRKPLLAFALAQAVLAFASAAVAQNDPAAPVYRCKAPGGAVTY
                     QDYACKGGIAVDIKPDAADPAAIQRLRQAQARFDRSLAQRRAAEMATRREPPERRRAT
                     ARAPGYDGDVQVPDASDVPGYLLYGPVPPAKLERRNRRIEHRVVAPGRRAVPGVIRRL
                     RPA"
     gene            complement(1777..1849)
                     /locus_tag="VFO53_13965"
     tRNA            complement(1777..1849)
                     /locus_tag="VFO53_13965"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1814..1816),aa:Thr,seq:cgt)
     gene            1936..3348
                     /locus_tag="VFO53_13970"
     CDS             1936..3348
                     /locus_tag="VFO53_13970"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8877431.1"
                     /translation="MNQLAMENVARLPRRLPRYLRAPPTAVSAAVVVALALLSIIAVI
                     AWRAIAAFEESSRWVAHSYTVIAELGQLESGHSRMRLAWRNYLTRPALDVLDDYRTSR
                     QALENGRAHLAALTQDNAAQHERIVALGDVIRQDIDSTGAAIGQKVAGSLDASSAILQ
                     AIGALRDNGERIGTLADAAESAERTLLAEREQATSDQGTRAKAWIIAGSVTSLVMLAL
                     AFLALRATEKQVRTLNGQLKSYSLQLEATNRELEGFSYSVSHDLRAPLRAINGFASML
                     EEDHGARLDAEGRRLLGVVRSNADAMACLIDDLLAFSRLGKVSFVRGPVDMEALVREV
                     LTERGGEAAIEIGTLPVAHGDRALLKQVWANLIDNAVKYSANVVTPVIRLSGFVHERG
                     DSEYRIDDNGAGFDMRYCDKLFGVFQRLHSASEFSGTGVGLAIVQRIVTRHGGRVWGE
                     GRPGAGATFHFRLPGEAAHE"
     gene            3341..3787
                     /locus_tag="VFO53_13975"
     CDS             3341..3787
                     /locus_tag="VFO53_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8877432.1"
                     /translation="MNDNFEMVDILLAEDSDADAEMTLRALRKSNIGNRVHRVRDGQE
                     ALDFLARTGAYAGASPSRHLKLVLLDLKMPKVDGAEVLRRLRADETFRTLPVVVMTSS
                     NEERDISQSYLLGANSYVVKPIDFTQFASTVVEIGLYWIVTNRVPG"
     gene            3774..6473
                     /locus_tag="VFO53_13980"
     CDS             3774..6473
                     /locus_tag="VFO53_13980"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET8877433.1"
                     /translation="MSPGSSPHAGDEAAPDAPLRLLIVEDVTADVELEMYELRRAGVA
                     ATCRVVDTEQGMRAAIPEFRPDVIISDFSMPHFDGLAALALARRIVPDVPFVFVSGTV
                     GEEAAIRALHAGASDYVLKANLRRLPSTIERCVKEARLHAERRRTEAALATLQQRLHD
                     VSTSLPDVLWSATLPGERVEFISRACETVYGRPADDFRANPNLWKEMIHPEDRARVAE
                     AWQQMRNGEPFDVEYRIVRADATLAWVNHRGRLVREGEDARFDGIVRDITGAVRDRKH
                     LARLAAIHAWLADTNAAIVRIRRDRELLQEVVRLAARITDIAGATATIFDVDSSPVHF
                     SVRAGVTAHAHDPQNAFAQSVRQSAGSALAVQLAGARRIWNDLADSDVPDRRELIALG
                     IRAVAAFPLAIDGRTVGEIQLFSWQPAFFEPAVAQLLREVAGNLSLALQLLSKQTEAD
                     YLALYDPLTGLPNRALLQSRLREFVSAAARSGSRLALVLLSVERMRDVNMLGGQQAGD
                     ELLRLLANRLRAIAGDEARVARLAGDHFALVVNDLVDAAALPGMLFGAGPGLLDIRHV
                     VHDREIHVTMRAGVAVYPSDGMDADALFQGAESALEDARMTRTRVAFCSPQLKAAAAE
                     RVDIEHAISTALSHDRFVLYYQAKVDLASGRICGVEALLRIADPDEGIIGPQRFIGVL
                     EASRLIDRVGRWVMEEALRAQARWRRRSGASPRVAVNVSAVQLLRAGFVDVVADVLKK
                     GGPEARLEFEITESVAVDDLEASVSVLARLRELGVTIALDDFGTGYSSLRYLSAMPLD
                     AVKIDRAFVAGLPASAGNASISSAILSLASALELTVIAEGVETEEQAQWLRVHGCPIA
                     QGYLFARPEPEERFADAWHADLARNGKFTPGEGHAPGSTRRSR"
     gene            complement(6424..7098)
                     /locus_tag="VFO53_13985"
     CDS             complement(6424..7098)
                     /locus_tag="VFO53_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HET8877434.1"
                     /translation="MTDDIRQFLETMLLALATLLPIVNPLGAAPVYLAKTIDLAANEH
                     ADLARRVAINCFLVLVASLLIGAYVLDFFGLSVPIVQVAGGLLVCSLAWSLLHQPDEP
                     LEWVHDAANTIRRPDLRTRAFYPLTMPLIVGPGSISVAITLGANQSHGFRPLIVDSLA
                     HATGALLVAVAIFVTLRYAEPILRRFGATGTAVLLRMSAFILLCIGVQIIWNGGSTLV
                     RALPPA"
     gene            7249..7989
                     /gene="rlmB"
                     /locus_tag="VFO53_13990"
     CDS             7249..7989
                     /gene="rlmB"
                     /locus_tag="VFO53_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET8877435.1"
                     /translation="MSATRPLFGFHAVTARLRQHAQSVEAVYVDAARHDMRIRDLAAR
                     ARAANVAIHTVDAARLAALAGRETHQGVVAIVDAQRPHVTLDDVLADLTEPPLLLVLD
                     GVTDPHNLGACLRCADAFGAQAVIVPKDRAAGLNATVAKAASGAAETVPLIPVTNLAR
                     TMRELKARDVWLLGADESGESLYGADVTGAIAWIIGAEGEGLRRLTRETCDRLIGIPM
                     QGAVESLNVSVTAGICLYSARAARSRSS"
     gene            7989..9608
                     /locus_tag="VFO53_13995"
     CDS             7989..9608
                     /locus_tag="VFO53_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877436.1"
                     /translation="MRFENVPPRGVFVTVALAFSLCASVGAAAKGTLVYCSEGSPEGF
                     QPQFFSTGTTFDAVSVPMFNRLVQFEIGTTNIVPALAESWTVAPDGKAYTFKLRKGVA
                     FHANANFKPTRDFNADDVLFSWYRMADDNHPFHKFTAGQTFAYFDDMGMKNVVDRVEK
                     LDDYTVRFVLKRPEAPFLADMAMDFASILSKEYFDAMMRKGTPNAADVYPIGTGPFEF
                     VSYQKDATIRYRAFDRYWNGRPKIDNLIYSITRDATARYAKLKTGECQVMAFPKPADL
                     DAMRNDPQLVIQHKEGLNVGYIAFNVEKKPFDSKLVRQALNYATNKDAILKAVYEGNG
                     QVAKNPIPPILWSYNDAVQDYPYDPAKAKALLAEAGYANGFAIDLWYLPVTRPYNPDG
                     KRMAELIQADWAKVCVKANLVTYEWAEYRKRSKTGEQAVMMFGWSGDNGDPDNFFVPL
                     LGCEAVKGGGNVSRWCNKPFEDLVIKAAQTPRQADRAKLYEQAQVIVHDEAPWIPIAH
                     SVRFDALRKDVRGYRMDATAHHYFDKVELAP"
     gene            9721..10728
                     /locus_tag="VFO53_14000"
     CDS             9721..10728
                     /locus_tag="VFO53_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8877437.1"
                     /translation="MSRFVLRRLALIVPTFLGVTLLAFALIHLIPGDPVENVSGERGM
                     DPARRARLLHEFGLDRPLIAQYASYVGQVLRGDLGSSLTTHEGVAHEFATLFPATVEL
                     ASFALLFALVVGLPAGVVAAVKRNTAADYAVMGVSLTGYSMPIFWWALLLILFFSVQL
                     GWTPVSGRIAVQYDIPAVTGFMLIDTLLAGDVDAFRSALSHLVLPTIALGTIPLAVIA
                     RMTRSAMLEVLREDFVRAARAKGLPRWRVIVVHALRNALIPVVTVVGLQVGLLLSGAI
                     LTETIFSWPGVGNWLIHGVQSRDYPVVQGGILLIAAIVIAVNLVVDMLYGVIDPRIRH
                     G"
     gene            10721..11650
                     /locus_tag="VFO53_14005"
     CDS             10721..11650
                     /locus_tag="VFO53_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8877438.1"
                     /translation="MASAPRSGALSAGSREGTAAPPSRGADFWRAFAQNRGALTGLAL
                     VVALVALALGADLVAPHSPIEQFRDFTLAPPFWDEGGSLRFALGTDAVGRDILSRLIH
                     GTRLSLLIGTISVSISLTLGCVLGLLAGYFRGLVDTAIMRLMDVMLALPSLLLAIAVV
                     AILGPGLINAMYAIAIVLLPHYVRLMRAAVIAEAGREYVTSSRIAGAGALRLMFSTIL
                     PNCVAPLIVQATLGFSNAILDAAALGFLGLGAQPPTPEWGAMLASALEFIQRAPWVVT
                     SPGLAILVTVLAFNLTGDGLRDALDPRLRRSAA"
     gene            11711..>12639
                     /locus_tag="VFO53_14010"
     CDS             11711..>12639
                     /locus_tag="VFO53_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8877439.1"
                     /translation="MSGPVEPLVEIRDLRVDFGTPARPLVAVDGVDLSIGPGEIVALV
                     GESGSGKSVAALALMGLVDAPAHVAARRIAFAGRDLQRMRASERRALAGRDVAMIFQD
                     PHASLDPCYTVGDQLDEALRFHGDAQERASAPVRRERALALLAEVEIVDAAARLRSYP
                     HQLSGGMAQRVMIAIALAGRPRLLIADEPTTALDVTVQAQVLALLLRLRDERAMAILL
                     ITHDLAVVAETADRIVVMYAGQVMETGTVPALFDAPRHPYTRALLDALPEHGAAGARL
                     RTIPGVVPGAAHRPAGCLLAPRCAFAVPRCTAER"
BASE COUNT         1994 a         4232 c         4290 g         2123 t
ORIGIN      
        1 gcgtctgatt gggggcgagt tgcgcaggcg catcgttggc ggcgaacacg atgcaagcgc
       61 ccgcggcgag tggcagcgcc cggtgagccg cgcgcaggat cgagcgcgcg atggatgcgg
      121 agttgcgcat ggagactcct cgttgcaatt gggaaggtgc agcacgatgc acgagctgcg
      181 cgggccttgc cgtgccggcc attcgcggct tgatgcgcgg tgcggcagtt accgcaaaca
      241 ggcgagacgt ttgcgcattc ccttgcgcct gaacggcgcg cgacgtcgcg ttccgcgtgc
      301 aggcaaacgc gagcgagcgc gcggaatgtt gcgcggccgc ggctgtttgc tcctggttgc
      361 catgcgtcgc atacccatac cgctgctttg cattgcgtgc gtccttgcat cgatgcaccc
      421 gattgcatcg cgcgcgagcg atcgggctgc cgacgtgcgc atcgagcgcc aaggcgatac
      481 gatggtcatc accgcgcatg cgcgcctcgt cgccgatcct gtcacggcgt gggcggtgct
      541 gaccgattac gcccgctatc gcgacttcat cccgggcgtc cgcgcgagcc gcgtcgtcga
      601 gcgtcgcgga agcgccgtcg tcgtcgagca atccgacgag ttcgcactct cgttcctgcg
      661 catgccggtt cgcgtcatgt acgagatcga cgagtttccc cgcgcacgat tgcagtcgcg
      721 agcacgcgcg tccgggttgc cgccgctatc cagcacgttc gtgctcgagc gcaccgaggc
      781 gggcgtgacg ctcgagtatg ccggttacat cggtcccggg ttgccgctcc tcggacgtct
      841 cgagcagccg gcgctgcagc gcgccgccat tcgcgatttc gaggcgctcg tggacgagat
      901 cgagcgcagg gcggcaacgc gcagcacgag gccgccccac gcggcccccg cccgcgttcc
      961 ggattgacat gtaccgacgg ctgctggcgg ccgccgcgct cgcagtccgc ttcacgcttc
     1021 gcgcccggcg cgtgcagtaa catgggagcg cgccttcccg cgcgcttcgg acccgaacaa
     1081 gccatgaaca tcaccctgag tgctgggccg ctgatttcgc tgattgccgg catcctgatc
     1141 ctgatcattc cgcggcttct caactacatc gtcgcgatct acctgattct gatcggactg
     1201 atcgggctct tcggcatcgg cgggctgcac gtgcgctgac cgccggcgcg ctgcgcgggc
     1261 gcagtcaggc cgggcgcaac cggcgaatga cgccggggac ggcccggcgt ccgggcgcaa
     1321 ccacccggtg ctcgatgcgc cggttgcgtc gctcgagctt cgccggcgga acgggcccgt
     1381 acaacagata tccgggaacg tcgctcgcgt cgggcacttg aacatcgccg tcgtagccgg
     1441 gtgcccgtgc ggtcgcgcgg cggcgctcgg gcggctcgcg acgcgttgcc atttccgcgg
     1501 ctctgcgctg cgccagcgaa cggtcgaatc gagcctgcgc ctggcgtagc cgctggatcg
     1561 cggcgggatc ggccgcgtcg ggcttgatgt cgaccgcaat gccgcccttg catgcgtagt
     1621 cctgatacgt caccgcgccg cccggtgcct tgcagcggta gactggcgcc gccggatcgt
     1681 tctgtgcaac ggccgccgat gcgaacgcga gtacagcctg ggcgagcgca aaggcgagga
     1741 ggggtttgcg aagcatgcgt cgcggtatcg gaagagtgcc ggtggaagga gtcgaaccct
     1801 cgaccttcgc attacgaatg cgctgctcta ccaactgagc tacaccggca agcagcgaaa
     1861 ttatagccga agtgttcgct tctccggcct cggccgaagc cttcggcaca gaacgtgctg
     1921 tcgatcgccg aggcaatgaa tcagctcgcc atggaaaacg ttgcgaggct tccgcgaagg
     1981 ttgcctcggt acctgagggc gcccccgact gcggtttcgg cggcggtcgt cgtagcactc
     2041 gcgctgctgt cgatcatcgc cgtcatcgca tggcgcgcca ttgccgcgtt cgaggaaagc
     2101 tcgcgctggg tcgcgcacag ctacacggtc atcgcggagc tcggacagct cgaaagcggg
     2161 cacagccgga tgcgtctcgc ctggcgcaac tacttgacgc gcccggccct cgacgtgctc
     2221 gacgattatc gaaccagccg tcaggcgctc gaaaacggcc gtgcgcacct cgccgcgctt
     2281 actcaggaca acgccgcgca acacgagcgg atcgtcgcgc tgggtgacgt gatacggcag
     2341 gatatcgact cgacgggtgc cgccatcggg cagaaggtcg cgggctcgct cgacgcttcg
     2401 tccgccatcc tgcaggcgat cggggcgtta cgcgacaacg gggagcgcat cggcacgctg
     2461 gccgacgccg cggaaagcgc cgagcgaacg ctcctcgccg agcgggagca ggcgacatcc
     2521 gaccagggca cgcgagccaa ggcatggatc atcgccggaa gcgtgacgag cctcgtgatg
     2581 ctcgcgctcg ccttcctggc gctgcgcgcc acggaaaaac aggtgcgcac gctgaacggg
     2641 cagttgaagt cgtactcgct gcaactcgag gcgaccaacc gcgagctgga aggtttctcc
     2701 tactccgtgt cgcacgatct gcgcgcaccg ctgcgcgcca tcaacggttt cgcctcgatg
     2761 ctcgaggagg accatggggc gcgtctggac gccgaaggcc gacgtcttct cggcgtcgtc
     2821 cggtcgaacg cggatgcaat ggcatgcctc atcgacgacc tgctcgcgtt ttcgcggctc
     2881 ggcaaggtgt cgttcgtccg cggtcccgtg gacatggagg cactcgtgcg cgaggtgctg
     2941 accgagcgcg gtggcgaggc ggccatcgag atcggcacgc tgcccgttgc gcacggcgat
     3001 cgagcgctct tgaagcaggt gtgggccaac ctgatcgaca acgcggtgaa atacagcgcg
     3061 aacgtcgtca cgccggtcat tcgcctgtcg ggcttcgtcc acgagcgcgg cgattcagaa
     3121 taccggatcg acgacaacgg cgcgggcttc gacatgcgct actgcgacaa gttgttcggc
     3181 gtgttccagc ggctgcattc ggcgagcgag ttctccggca ccggcgtcgg tcttgccatc
     3241 gtgcagcgca tcgtcacgcg ccacggcggg cgcgtctggg gcgagggccg tccgggcgcg
     3301 ggcgcaacgt ttcacttcag gctcccggga gaagctgcgc atgaatgaca acttcgaaat
     3361 ggtcgatatc ctgctcgccg aggacagcga cgccgatgcc gagatgacgt tgcgggcact
     3421 gcgcaagagc aacatcggaa acagggtgca ccgggtgcgc gacgggcagg aagcgctcga
     3481 tttcctggcg cgcaccggcg catacgcagg cgcttcgccg tcgcggcacc tcaagctcgt
     3541 gctgctcgac ctcaagatgc cgaaggtcga cggcgccgag gtcctgcgcc ggctccgcgc
     3601 cgacgagacg tttcggacgt tgcccgtggt cgtgatgacg tcgtcgaacg aggagcgcga
     3661 catttcgcag agctatcttc tcggcgccaa cagctacgtc gtgaagccga tcgatttcac
     3721 gcagttcgcg agcacggtcg tcgagatcgg cctttactgg atcgtcacca accgtgtccc
     3781 cgggtagctc tccgcacgca ggcgacgagg cggcacccga cgcgccgctg cggcttctga
     3841 tcgtcgagga cgtcaccgcc gacgtcgagc tcgagatgta cgagctgcgt cgtgcgggcg
     3901 tggccgccac ctgccgggtc gtcgataccg agcaggggat gcgcgccgct atccccgagt
     3961 ttcggccgga cgtgatcatt tccgatttct ccatgccgca tttcgacggg ctcgcggcac
     4021 tggcgcttgc ccgccgcatc gtgcccgacg tccccttcgt tttcgtgtcg ggcaccgtcg
     4081 gcgaagaggc ggcgattcgc gcgctgcacg cgggtgccag cgattacgtc cttaaggcga
     4141 atctgcgccg cctcccgtcg accatcgaac gttgcgtgaa ggaagcgcgg ctgcacgcgg
     4201 aaaggcggcg gacggaggcg gcactcgcga cgctccagca gcggctgcac gatgtgtcga
     4261 cgtcgcttcc cgacgtgctg tggtcggcga ccttgcctgg cgagcgggtc gaattcatca
     4321 gccgggcatg cgagaccgtc tatggccggc cggcggacga ttttcgggcc aacccgaatc
     4381 tgtggaagga aatgatccac cccgaagacc gtgcacgcgt tgccgaagcg tggcagcaaa
     4441 tgcgcaacgg ggagccgttc gacgtcgaat accggatcgt gcgcgccgac gccacccttg
     4501 cctgggtgaa ccaccgcggc cgcctcgtgc gcgagggtga ggacgcacgc ttcgacggca
     4561 tcgttcgcga catcacgggc gcggtccgcg accggaagca tctcgcgcgc cttgctgcca
     4621 ttcatgcgtg gctcgccgat acgaacgcgg cgatcgttcg catccggcgc gatcgcgagc
     4681 tgctccagga agtggtccgg ctcgcggcgc gcatcaccga catcgccgga gccaccgcaa
     4741 cgatcttcga cgtcgacagc agcccggtgc atttttcggt gcgcgccggc gtgacggcgc
     4801 acgcacacga tccgcagaac gcgttcgccc agagcgtgcg gcagagtgcg ggaagtgcac
     4861 tcgcggtcca gctcgccggc gcacggcgca tctggaacga cctcgccgac agcgacgtgc
     4921 ccgatcgacg cgagctgatt gcgctcggca tccgggccgt ggccgcgttt ccgctcgcga
     4981 tcgacggccg caccgttggc gagatccagc ttttctcgtg gcagccggcc tttttcgagc
     5041 cggccgtcgc gcaactgctg cgcgaggtcg ccggaaacct gtcgctcgcg ctccagctcc
     5101 tctcgaagca gaccgaagcc gactatcttg cgctgtacga cccgttgacg ggcctgccga
     5161 atcgcgccct gctgcagagc cgcttgcgcg agttcgtgag cgctgccgcg cgcagcggaa
     5221 gccgccttgc gctggtgctc ctttccgtcg agcgcatgcg ggacgtcaac atgctcggcg
     5281 ggcaacaggc cggcgacgaa ttgttgcggc tgctcgcaaa ccggctgcgt gcgattgccg
     5341 gggacgaggc gcgggtcgca cgccttgccg gtgaccactt cgcactcgtc gtcaatgacc
     5401 tcgtcgacgc cgcggcgctg cccggaatgc tctttggtgc cggacccggg ctgctggaca
     5461 ttcgccacgt cgtgcacgat cgtgaaattc acgtgacgat gcgtgccggc gttgccgtct
     5521 acccgtccga cggcatggat gcggatgcct tgttccaagg tgcagaaagc gcgctcgagg
     5581 acgcacggat gacacgcacg cgcgtcgcct tctgctcgcc gcaactcaag gctgcggccg
     5641 ccgagcgtgt cgacatcgag catgcgatct cgacggcgct gtcgcacgac cgcttcgtgc
     5701 tgtactacca ggcgaaggtc gatctcgcca gcggccgcat ctgcggcgtc gaggcgctcc
     5761 tgcgcatcgc agaccccgac gaggggatca tcgggccgca acgattcatc ggcgtgctcg
     5821 aggcatcgag gctcatcgac cgcgtgggac gctgggtgat ggaggaagcg ctccgcgcgc
     5881 aggcgcgctg gcgcaggcgc agcggcgcca gcccgcgcgt cgccgtcaac gtctcggcgg
     5941 tgcagttgct gcgcgccggc ttcgtcgatg tcgttgccga cgtgctgaag aaaggcggtc
     6001 ccgaggcccg cctcgagttc gagatcaccg agtcggtcgc ggtggacgac ctggaggcgt
     6061 cggtcagcgt gcttgcgcgg ctgcgcgagc tcggcgtcac gatcgccctc gacgatttcg
     6121 gcaccggcta ttcgtcattg cgttacctga gcgccatgcc gctcgatgcc gtgaagatcg
     6181 accgcgcgtt cgtcgcgggg ctgccggcga gtgccggcaa cgccagcatc tcgagtgcga
     6241 tcctgtcgct cgccagcgcc ctcgagctca cggtcatcgc cgagggggtc gaaaccgagg
     6301 agcaggcgca atggctgcgc gtgcacggtt gtccgatcgc tcaaggctat ctcttcgcac
     6361 gccccgagcc cgaagagcgt ttcgccgacg cctggcatgc cgatctcgct cgcaacggca
     6421 aattcacgcc gggggaaggg cacgcaccag ggtcgacccg ccgttccaga tgatctgcac
     6481 gccgatgcag agcaggatga atgccgacat gcgcagcagc accgccgttc ccgttgcgcc
     6541 gaagcggcgc aggatcggct cggcgtagcg cagcgtgacg aagatcgcga cggcgacgag
     6601 cagcgcgccc gtcgcatgcg cgagcgaatc gacgatcaac ggccggaagc cgtgcgactg
     6661 gttcgcgccg agcgtgatgg cgaccgaaat cgatccggga ccgacgatca gcggcatcgt
     6721 cagcgggtag aacgcgcggg tgcgcagatc ggggcggcgg atcgtgttcg cggcatcgtg
     6781 cacccattcg agcggctcgt cgggctggtg cagcagcgac cacgcgagcg agcagacgag
     6841 gagcccgccc gcgacctgga cgatcggcac cgacaggccg aagaagtcga gcacgtaggc
     6901 gccgatcaaa agcgatgcga ccagcacgag aaagcagttg atcgcaacgc gtcgcgcgag
     6961 atcggcgtgc tcgttcgcgg cgagatcgat cgtcttggcg agatagaccg gcgccgcgcc
     7021 aagcggattg acgatgggca gcagcgtcgc aagcgcgagc agcatcgttt cgaggaactg
     7081 gcgaatatcg tcggtcatgc ggtcgggaat gttggcgtcg tgggcgggga agattcgcgg
     7141 cgtgggggca gggcgccggc gattctgtca caacccggcg taacgccccc gggcggtaga
     7201 atccacgccc gtcccaccgt ttccaccgac gcgaagcgcc ctcacgccgt gtccgccaca
     7261 cgaccgctgt tcggcttcca cgccgtcacc gcgcgcttgc gccagcacgc gcagtcggtg
     7321 gaggcggttt acgtcgacgc ggcacgtcac gatatgcgca tacgcgatct cgctgcgcgt
     7381 gcgcgagcgg cgaacgtggc gatccacacg gtggacgccg cccgccttgc ggcgctcgcg
     7441 ggtcgtgaga cgcaccaggg cgtggtcgcg atcgtcgacg cgcagcgccc gcacgtcacg
     7501 ctcgacgacg tgctggcgga tttgacggaa cccccgctgc tgctcgtgct cgacggcgtg
     7561 accgatccgc acaacctcgg cgcatgcctg cgctgcgccg acgccttcgg cgcgcaggcc
     7621 gtcatcgtgc cgaaggaccg cgcggccggc ctcaatgcga cggttgcgaa ggcggcaagc
     7681 ggtgcggccg agacggttcc actgattccc gtgacgaacc tcgcgcgtac gatgcgcgag
     7741 ttgaaggcgc gcgacgtctg gctgctcggc gccgacgaaa gcggcgaaag cctctatggc
     7801 gccgatgtca ccggcgcgat cgcgtggata atcggcgccg agggcgaggg gctgcgccgg
     7861 ctgacgcgcg aaacctgcga ccggctgatc ggcattccga tgcaaggcgc cgtcgaaagc
     7921 ctcaacgtgt cggtcaccgc cggcatttgt ctctattccg cgcgagccgc gcgatcgagg
     7981 tcatcatgat gcgtttcgaa aacgtgcctc cccgcggcgt cttcgtcacg gttgcgcttg
     8041 cattctcgct gtgcgcatcg gtgggagccg ctgcgaaggg aacgctggtc tattgctcgg
     8101 aaggcagccc cgagggtttc cagccgcagt tcttcagcac cgggacgacg ttcgatgcgg
     8161 tgtcggtgcc gatgttcaac cggctcgtcc aattcgagat cggcacgacg aacatcgtgc
     8221 cggcgctcgc cgaatcgtgg accgtcgcgc cggacggcaa ggcgtacacg ttcaagctgc
     8281 gcaagggcgt ggcgttccac gcgaatgcga acttcaagcc gacgcgcgac ttcaacgcgg
     8341 acgacgtgct gttctcctgg taccggatgg ccgacgacaa ccatccgttc cacaaattca
     8401 ccgccgggca gacgttcgcg tatttcgacg acatgggcat gaagaacgtc gtcgacagag
     8461 tcgagaagct cgacgattac acggtgcgct tcgtgctgaa gcgtcccgag gcgccgtttc
     8521 ttgccgacat ggcgatggat ttcgcgtcga tcctgtcgaa ggaatacttc gacgcgatga
     8581 tgaggaaggg aacgccgaac gccgccgacg tctatccgat cggcaccggc ccgttcgagt
     8641 tcgtgagcta tcagaaggac gcgacgatcc gctacagggc gttcgatcgc tactggaacg
     8701 gccggccgaa gatcgacaac ctgatctatt cgatcacgcg cgatgccacc gcccgttacg
     8761 cgaagctcaa gacgggcgag tgccaggtga tggcgtttcc gaaaccggcc gatctcgacg
     8821 cgatgcgcaa cgacccgcaa ctggtcatcc agcacaagga aggtctcaac gtcggctaca
     8881 tcgcgttcaa cgtcgagaaa aagccgttcg acagcaagct cgtgcgccag gcgctgaact
     8941 acgcgacgaa caaggatgcg atcctcaagg ccgtctacga gggcaacggc caggtggcga
     9001 agaatccgat tccaccgatc ctgtggagct acaacgacgc ggtgcaggat tacccgtatg
     9061 accccgccaa ggcgaaggcg ctgcttgcag aggccgggta cgccaacggc ttcgcgatcg
     9121 acctctggta tctgccggtc acgcgcccgt acaacccgga tgggaagcgg atggccgaac
     9181 tgatccaggc cgactgggcg aaagtctgcg tcaaggcgaa tctcgtcacg tacgagtggg
     9241 cggaataccg caagcgcagc aagaccggcg agcaggccgt catgatgttc ggctggtcgg
     9301 gcgacaacgg cgatcccgac aatttcttcg tcccgctgct gggctgcgag gcggtcaagg
     9361 gcggcggcaa cgtctcgcgc tggtgcaaca agccgttcga ggacctggtc atcaaggcgg
     9421 cgcaaacgcc gcggcaggcc gaccgcgcga aactctacga gcaggcgcag gtcatcgtcc
     9481 acgacgaagc gccgtggatc ccgatcgcgc actcggtgcg cttcgatgca ttgcgcaagg
     9541 acgtgcgcgg ctacaggatg gacgccacgg cgcatcacta tttcgacaag gtcgagctcg
     9601 cgccgtgatg acggcagtcc cgtgccgccg cacccttcgc gccgccgggc cgtcccaagg
     9661 cgcgaatcgc gccccctcgg ggggcagcga agcggcgaga gccgcgagcg tgggggccca
     9721 atgtcgcgct tcgtgctgcg ccgcctggcg ctgatcgtcc cgacgtttct cggcgtcacg
     9781 ctgctggcgt tcgcgctgat ccacctgatt cccggcgacc cggtcgagaa cgtctccggc
     9841 gagcgcggga tggatccggc gcggcgtgca cgcctgttgc acgaattcgg cctcgaccgc
     9901 ccgctgatcg cgcagtacgc aagctacgtc ggtcaggtgc tgcgcggcga tctcggcagc
     9961 tcgctcacca cgcacgaggg cgtcgcgcac gaattcgcga cgctttttcc cgccacggtc
    10021 gagctcgcat cgtttgcgct gctgttcgcg ctcgtcgtcg gattgccggc gggcgtcgtc
    10081 gctgccgtga agcgcaacac ggcagccgat tacgcggtga tgggcgtgtc gctcaccggc
    10141 tattcgatgc cgattttctg gtgggcgctg ctgctgatcc tgttcttctc ggtgcaactc
    10201 ggctggacac cggtctccgg aaggattgcc gttcagtacg acatccctgc ggtcaccggc
    10261 ttcatgctga tcgacacgct gctcgccggc gacgtcgatg cattccgttc ggcgctgtcg
    10321 cacctcgtgc tgccgacgat cgcgctcggc acgattccac tcgcggtcat cgcacggatg
    10381 acgcgctcgg cgatgctcga ggtgctgcgc gaggatttcg tgcgggccgc gcgcgccaag
    10441 gggctgccgc gctggcgcgt gatcgtcgtg catgcgctgc gcaacgcgct gattccggtc
    10501 gtgaccgtcg tcggcctgca ggtcggcctg ctgctgtccg gcgccatcct caccgaaacg
    10561 atcttctcgt ggccgggcgt gggcaactgg ctgatccacg gcgtgcagtc gcgcgattac
    10621 ccggtggtgc agggcggcat cctgctgatc gcggcgatcg tcatcgccgt gaatctcgtc
    10681 gtcgacatgc tgtacggcgt gatcgacccg cgcatccggc atggctagcg cgccacgctc
    10741 cggtgcgctt tcggccggct cgcgcgaggg cacggccgcg ccgccatcgc ggggcgccga
    10801 cttctggcgc gcgtttgcgc agaatcgcgg cgcgctcacc ggactcgcgc tcgtggttgc
    10861 gctcgtcgcg ctggcactcg gtgccgatct cgtcgcgccg cattcgccaa tcgagcaatt
    10921 ccgcgatttc acgctcgcac cgccgttctg ggacgaaggc ggcagcctgc gctttgcgct
    10981 cgggaccgat gcggtcggac gcgacatcct gtcgcgcctg atccacggca cgcggctgtc
    11041 gcttttgatc ggcacgatct cggtctcgat ctcgctgacg ctcggctgcg tcctcggtct
    11101 tctcgccggg tattttcgcg ggctcgtcga cacagcaatc atgcgcctca tggacgtcat
    11161 gctggcgttg ccgagcctgc tgctcgcgat cgctgtcgtg gccatcctcg gacccggcct
    11221 catcaacgcg atgtatgcga tcgcgatcgt gcttttgccg cattacgtgc gcctgatgcg
    11281 cgcagcggtg atcgccgagg cgggccgcga gtacgtgacg tcgtcgcgca tcgcaggtgc
    11341 cggcgcgctg cgtctcatgt tcagcacgat cctgccgaac tgcgtggcgc cgctgatcgt
    11401 gcaggccacg ctcggctttt ccaacgcgat cctcgacgcc gcggcgctgg gcttcctcgg
    11461 cctcggcgcg cagccgccaa cgcccgaatg gggcgcgatg ctcgcctcgg cgctcgagtt
    11521 catccagcgc gcgccatggg tcgtgacctc gccgggtctt gcgatcctcg tcaccgtgct
    11581 cgccttcaac ctgaccggcg acggtctgcg tgacgcgctg gatcccaggt tgcgccggtc
    11641 ggccgcatga gcgcgaaggg ccgccccgag cgcgaatcag ccccgcagcg cgaagcgcgg
    11701 aggtagtcca atgagcggac cggtcgagcc actcgtcgag atccgcgatc tgcgcgtcga
    11761 tttcggcacg ccggcacgcc cgctggttgc cgtcgacggt gtcgacctgt cgatcggccc
    11821 cggcgagatc gttgcgctcg tcggcgaatc gggatccggc aagagcgtcg cagcactcgc
    11881 gttgatggga ctcgtcgatg cgccggcgca tgtcgccgca cggcgcatcg ccttcgcggg
    11941 acgcgacctg cagcggatgc gcgcctcgga gcgtcgcgcg ctggccggcc gcgacgttgc
    12001 gatgatcttc caggacccgc acgcgagcct cgacccgtgt tacaccgtcg gcgaccagct
    12061 cgacgaagcg ctgcgcttcc acggcgacgc gcaggaacgg gcgagcgcgc cggtacggcg
    12121 cgaacgtgcg ctcgcgctgc ttgccgaggt cgaaatcgtc gacgcagccg cgcggctgcg
    12181 ttcctatccg catcaattgt cgggcggcat ggcgcagcgc gtgatgatcg ccattgcgct
    12241 cgccggacgg ccgcggctgc tgatcgccga cgagccgacg accgcactcg atgtcacggt
    12301 gcaggcgcaa gtgctcgcgc tgcttctgcg cctgcgcgac gagcgcgcga tggcgatcct
    12361 gctcatcacg cacgacctcg ccgtcgttgc cgaaaccgcg gatcgcatcg tcgtgatgta
    12421 tgccggacag gtaatggaaa cgggaacggt gccggcgctg ttcgacgcgc cgcgccatcc
    12481 gtacacgcgc gcattgctcg acgcattgcc cgaacacggc gcggccggcg cgcggctgcg
    12541 cacgattccc ggcgtcgtgc ccggcgccgc gcatcggccg gccggttgcc tgctcgcgcc
    12601 gcgctgcgcg ttcgctgttc cacgatgcac ggcggagcg
//
LOCUS       DASSVX010000328        33117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_914956, whole genome shotgun sequence.
ACCESSION   DASSVX010000328 DASSVX010000000
VERSION     DASSVX010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 33117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33117
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914956"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..636)
                     /locus_tag="VFO53_14015"
     CDS             complement(124..636)
                     /locus_tag="VFO53_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013951778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HET8877440.1"
                     /translation="MRRRLYFIMPDLASARKTMDDLLLARIEARHIHFLARRGTPMDG
                     LNEANHLQKSDLVHGAQVGLALGALLGFILGGVLVMTLVTEDRWQIVTVLGAGIIGAL
                     FGAWVASMVGVAVPNSRLRQFSQAIDEGRLLLMADVPEHRVDEIRKLLNAQHPEAEDR
                     GIDPHIPAFP"
     gene            727..1320
                     /locus_tag="VFO53_14020"
     CDS             727..1320
                     /locus_tag="VFO53_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET8877441.1"
                     /translation="MRVVGILLAAGRGTRFGGDKLAAPLPRVSHGVPAGTPLGVAAAM
                     HLVDALPDSVAVVRPGDRLTEALRRAGPRIVECRNADDGMGASLACGVAATPDALGWI
                     IALADMPWIAPATIRAVANALRDGADIVAPVHRGERGHPVAFAARHGGALAALRGDAG
                     ARSLLAAHAGAVTLLGVDDDGVVRDVDTRQALAARDR"
     gene            complement(1328..2848)
                     /locus_tag="VFO53_14025"
     CDS             complement(1328..2848)
                     /locus_tag="VFO53_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HET8877442.1"
                     /translation="MAQIAQEDLIQSVADALQFISYYHPADYIAALGDAYAREESAAA
                     KDAIAQILTNSRMCAEGHRPICQDTGIVVAFVRVGMDARFAGATMSVADMVDAGVRRA
                     YLDPDNTLRASIVRDPASTRVNTRDNTPAVVHFDIVPGDTVDVRIAAKGGGSENKARF
                     AMLDPSDSIVDWVVESVPGMGAGWCPPGMLGIGIGGSAEKAMLLAKEALMAPIDMHAL
                     KARGPSNRLDELRIELYDKVNALGLGAQGLGGLSTVLDVKILDYPTHAASLPVAMIPN
                     CAATRHAHFTLDGNGIAKLDPPDLATWPDVQWSPAPQSRRVNVDTLTRDDVARWTPGE
                     RLLLSGRILTGRDAAHKRIADMLARGHALPVDFTGRVIYYVGPVDAVRGEAVGPAGPT
                     TSTRMDKFTETMLAKTGLIAMIGKAERGPVAIDAIRRHRAAYLIAVGGAAYLVSKAIR
                     RSRVVAFADLGMEAIHEFDVVDMPVTVAVDSTGTSVHETGPREWKQRIATLHPLAT"
     gene            2938..4917
                     /gene="acs"
                     /locus_tag="VFO53_14030"
     CDS             2938..4917
                     /gene="acs"
                     /locus_tag="VFO53_14030"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET8877443.1"
                     /translation="MSNIRSVMNEERVFEPPRGFAAQANVTKARAEAMRAKAARDYEG
                     FWAELARDTLTWKKPFTKVLDEGKAPFYQWFNDGELNVSYNCLDRNLENGNAGKTAII
                     FEADDGAVTRVTYRELHARVCRLANGLRSLGVAKGDRVVIYMPMSVEGIAAMQACARI
                     GATHSVVFGGFSAKSLHERIVDAGAVAVITADEQMRGGKPLPLKRVVDEALGMGGCDA
                     VRHVVVYRRTGAEVSFAAGRDVWMHELAAKQADTCEPAWVGAEHPLFILYTSGSTGKP
                     KGVQHSSAGYLLWCALTMQWVFDMKPDDVFWCTADIGWVTGHSYITYGPLACGATEVV
                     FEGIPTYPDAGRFWKLIADHRVSVFYTAPTAIRSLIKANNVNPSTAPSNYDLASLRLL
                     GSVGEPINPEAWMWYYTNVGRERCPVVDTWWQTETGGLMISPLPGVTPLVPGSCTVPL
                     PGIMAAIVDETAHAVPNGQGGMLVIERPWPGMIRTIWGDPQRYVKSYFPDELGGRLYL
                     AGDGAVRNAKTGYFTIMGRIDDVLNVSGHRLGTMEIESALVANPIVAEAAVVARPDDL
                     TGEAICAFVVLKRTRPHDGEAADVAKALRDWVGKEIGPIAKPKDIRFGDNLPKTRSGK
                     IMRRLLRSLARGEEITQDVSTLENPAILEQLKQSL"
     gene            complement(5002..5613)
                     /locus_tag="VFO53_14035"
     CDS             complement(5002..5613)
                     /locus_tag="VFO53_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET8877444.1"
                     /translation="MKLIASTTSPYARKVRIALAEKKIEYQMVESSGWAPGNPVHAWN
                     PLGKLPVLILDDGTHLYDSRVIVEYIDLVSPVSRLIPEPARQRIAVKKWEALADGVCD
                     ASSAIVIERRRPANLQSDEWIARQRRKIDEGTAELARELGERAWCHGEGYSLADIATG
                     AALGYLDLRFAELDWRDLYPNLARLADKLDKRPSFADTAPPHA"
     gene            5695..7074
                     /gene="purB"
                     /locus_tag="VFO53_14040"
     CDS             5695..7074
                     /gene="purB"
                     /locus_tag="VFO53_14040"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006377017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET8877445.1"
                     /translation="MDAADTARLTALSPLDGRYAQKVDALRGCFSEFALIRDRVRVEI
                     AWLLALSDEPAIGEVPPFSAAAKAVLADIGASFREADAARVKAIERTTNHDVKAVEYW
                     LKERVAGEPQIARVGEFIHFACTSEDINNLAYGIALAQARRAVLLPTLEAIAGDLRQM
                     ATAYAAWPMLARTHGQPATPTTLGKELANVYARLERQIATFARIPIKGKMNGAVGNYN
                     AHTLAYPDVDWERLAAKTVTGLGLEFSPYTTQIEPHDGVAEYCDALARTNTILIDLDR
                     DVWGYVSLGYFRQRTREGEVGSSTMPHKVNPIDFENSEGNLGLANALLRHLAEKLPVS
                     RWQRDLTDSTVLRNVGVALGHAQLGYVALRQGLAKLDADRERMANDLDANWEVLAEAI
                     QTLMRRYGLPEPYEQLKALTRGKSGMTRESLQAFIDALALPDDAKARLRALTPATYVG
                     LAEELARRV"
     gene            complement(7081..7746)
                     /locus_tag="VFO53_14045"
     CDS             complement(7081..7746)
                     /locus_tag="VFO53_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET8877446.1"
                     /translation="MLTTLLYSLLALDQTVASLATQYGAWLYAILFVVIFAETGLVVC
                     PFLPGDSILFIAGTVVAAAGLDVHVLVAVLVAAAVLGDSVNYGIGHYIGPKAFHERDS
                     RWFRQEYLRRTQSFYEKYGAVTIIIGRFVPIIRTFAPFLAGVAGMQYRRFLSYNVIGG
                     CLWIGLLVYAGFLFGNIPWVKDNLSLIVIAIVVVSLIPAVTTFVKERRAATKPPASPP
                     RVE"
     gene            complement(7757..8875)
                     /locus_tag="VFO53_14050"
     CDS             complement(7757..8875)
                     /locus_tag="VFO53_14050"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HET8877447.1"
                     /translation="MADLAFKSSSALTFGVELELMLVNTRDFNLAPDADDLLRRITRD
                     APQGELKPELTQSMIEINTSVHERYPQLLAELRSTRDVLAGHARKMNVALAGGGTHPF
                     QKWSERKIYPNERFLSVSEKYGFLAKQFTVFGQHVHIGCASADDAIYLTHALIRYVPH
                     FIALAASSPFYQGVDTAFDSSRLSIVNAFPLSGTMPLVRSWSEFNRYYDRMVALGIVK
                     SMKDFYWDIRPKPEYGTVEIRVCDTPLTVDRAAQIAAFAQALARWLWEERPVDVTPDI
                     YLPHSYNRFQACRHGFAGTLVDATTGRSRSLAEDLSETLATLAPHAQMLDSQAALTAL
                     GELVERGNHAAWLRRAFDETATLTDVVRRQSELWAAAD"
     gene            complement(8881..10089)
                     /locus_tag="VFO53_14055"
     CDS             complement(8881..10089)
                     /locus_tag="VFO53_14055"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET8877448.1"
                     /translation="MPDFLPAWPPAANSFLIFGLLLLAGLAGGRLAATTRVLPAITGY
                     IATGFLLGPGVLGWLSESALRDSRVLAEFSLGLIVFDLGRRLDLSWARHDRWLLPMGL
                     AESALSFLAMFALLHFAGGVGMLEAAVAAMVGTATAPAVVLLVTNELKAEGPVTRRML
                     WHVALNNIIATIGITLLLPFVEARMSGATLNPLGRALWLIAGSFLLGLVAFQVMLLLA
                     RWLGKSPVPQFLLAVGMIVATVGTAQLGRLSVLLALLVLGACTRNLDRKHRLIDVEFG
                     RAAQLFFVALFVLTGATLRPDQFGAVAWIGLGFVVVRLLGKAVGLYALAWPAHVSMRQ
                     AGTLALALTPMAGLAIGMTQPIYDIAPEFGARLAAIVVSGIAILHLLGPIATRYALIR
                     AGEGEPGARD"
     gene            complement(10093..11034)
                     /gene="secF"
                     /locus_tag="VFO53_14060"
     CDS             complement(10093..11034)
                     /gene="secF"
                     /locus_tag="VFO53_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET8877449.1"
                     /translation="MEFFRISRDIPFMRHALVFNVVSIITFVAAVVFLAIYGLNLGVD
                     FRGGTVIEVNYGHAVDFGRVRSAIDKLGLGEFSVQSFGSSETALIRLPLKAGLSSAQL
                     SDRVMGALKADDPTASQRRVEFVGPQVGKELYENGALALLLVSIGIVIYLALRFEWRF
                     AVAAIIANLHDVVIILGFFALFQWEFSLPVLAAVLAVLGYSVNESVVIADRIRENFRK
                     MRKASVTHVIDSAITRTLSRTIITHGSTLLMVLSIFFFGGETLHHFSLALAIGICFGI
                     YSSALVMASLVMWLGVSREDLVKSDRKRAVAGEKILP"
     gene            complement(11065..12930)
                     /gene="secD"
                     /locus_tag="VFO53_14065"
     CDS             complement(11065..12930)
                     /gene="secD"
                     /locus_tag="VFO53_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET8877450.1"
                     /translation="MNRYPLWKYIVIAIALVVGIVYMVPNFFPDVPAVQVSSSKSAVK
                     VDTALLGTIEDALKSASIPYRGAELDPVGIKVRLPDLDTQVRAKDVIAQKLGDNYIVA
                     LNLLSSSPSWLTSIGALPMYLGLDLRGGVYFLLQVDMKGALDKAADRYTTDIRSLMRE
                     KKVLYSGVGREGSNVVLRFKDAAERTRARIEIETAFADLDVKEQDAGSGELRLVAGLK
                     PQAQTRISEGAVQQNITILRNRVNELGVAEPIIQQQGSDRVVVQLPGVQDTARAKDIL
                     GRTATLEIRMVNDDPGALEAARAGSVPFGNDLYTDRNGEPILVKRQVVLTGDRISDAQ
                     SGFDQRTNEPAVHVNLDGTGARIFKEVTRENVGKRMAILLVEKGKAEVITAPVIREEI
                     GGGRVQISGRMNTREANDIALLMRAGALAAPMEIVEERTVGPSLGKENIAKGFDSVKY
                     GFLVLAAFICVYYMLMGIISTAALAINLMLLVAILSLLQATLTLPGMAAIALTLGMAI
                     DANVLINERIREELRWGATPHAAIQAGYERAWGTILDSNVTTMIAGIALLAFGSGPVR
                     GFAVVHVLGILTSMFSAVFVSRGIVALIYGSRRKLDHISVGQAWHPEPEASMVKS"
     gene            complement(12968..13294)
                     /gene="yajC"
                     /locus_tag="VFO53_14070"
     CDS             complement(12968..13294)
                     /gene="yajC"
                     /locus_tag="VFO53_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET8877451.1"
                     /translation="MLISPAYAQAAAGAAQENTLLTFLPMVAIFIVFYFLLIRPQQKK
                     QKEARAMLESLEKGNEVVTAGGILGRIVKLDEQYATVEVAPNVQMTVQRGSISQLLPK
                     GTLKGL"
     gene            complement(13408..14520)
                     /gene="tgt"
                     /locus_tag="VFO53_14075"
     CDS             complement(13408..14520)
                     /gene="tgt"
                     /locus_tag="VFO53_14075"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET8877452.1"
                     /translation="MSFAVLGRCGAARRARLTLAHGDVETPVFMPVGTYGTVKAMSPA
                     ELTALGAQIILGNTFHLWLRPGTDVIGAHGGLHRFAGWNKPILTDSGGFQVWSLGALR
                     KVHEDGVTFASPVNGDRLFLSPEISMEIQRVLDSDIVMVFDECTAFPAAHDEAAVSME
                     LSLRWAARSKAAHAGNPNLLFGIVQGGMYEDLRDRSRDGLLEIGFDGYAIGGLSVGEP
                     KDDMLRVLRHTAQRLPDDKPRYLMGVGTPEDIVAGVLAGVDMFDCVLPTRNARNGWLF
                     TRHGDIRIRNARHRDDTAPLDPTCECYACRHFSRAYLHHLQRINEILGARLATIHNLH
                     YYLTLMAELRAAIESRTLADYALRFEAERRGDALRC"
     gene            complement(14530..15480)
                     /locus_tag="VFO53_14080"
     CDS             complement(14530..15480)
                     /locus_tag="VFO53_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877453.1"
                     /translation="MSSVFRIIAAVVAVFSFCTVAHAARVAILSNAWSAETAADYNAK
                     IGGHVFTGIDVTSSVPTLDLLLANFDVVLLFEDRVFVNATAVGNRAAEFANAGRAVVL
                     GTFYDQDRSDATAGAATPHGWGALEGIDPNTTDGVGTAYAVRTLAPASIVAHPLTRGV
                     TSLAALRGNPGPYAGGNQAKPGTSVVAAWAQPNARGAVDPAIAYRQTGPACVIHIGIA
                     PQYGVLPAFGTYGTDFAGDFYQVWTNAFDFAAAMCKIAPAIVPGDFGQIGRGWASRAE
                     LDAWRTAFSFAIVLDGRFLQGGFGPALPLTTHPDGTITAE"
     gene            complement(15713..16759)
                     /gene="queA"
                     /locus_tag="VFO53_14085"
     CDS             complement(15713..16759)
                     /gene="queA"
                     /locus_tag="VFO53_14085"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET8877454.1"
                     /translation="MSSHLPWTLDDFDYDLPAELIAQSPAPQRTASRLLHVDGTKLDD
                     LRFADFPRLVGDDDLLVLNDTRVINARIRGRKPTGGDVEMLVERVSERDAALVQLRAS
                     HPPGAGGTIELAAGARATVIGRDGRFFRLRFEGVDDLPRWLEQHGELPLPPYITHAPG
                     VADAERYQTVYARHPGAVAAPTAGLHFDRAMLAALAKRGVATAWVTLHVGAGTFQPVQ
                     SHLIAEHRMHSEWYSIAPETVHAIAGTRARGGRIVAVGTTSLRALEAAAGEDGAIRAG
                     SSQTSLFIMPGYRFRVVDRLLTNFHLPKSTLLMLVSAFGGHAAIRGAYAHAISQRYRF
                     MSYGDAMLLEHGNR"
     gene            complement(16795..16880)
                     /locus_tag="VFO53_14090"
     tRNA            complement(16795..16880)
                     /locus_tag="VFO53_14090"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16845..16847),aa:Leu,seq:caa)
     gene            complement(16953..17525)
                     /locus_tag="VFO53_14095"
     CDS             complement(16953..17525)
                     /locus_tag="VFO53_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HET8877455.1"
                     /translation="MSAHEFHVHGPHDHEVEHAAHERDPFSARIAVMTAIFATIGALF
                     GYMVGATQNDALLFKNDSAIRRTEASDQWNFYQAKSSKQNLAELGASLATGDAGARFK
                     AEAERYNREKQDIMTQAQKLEKRSEELEAKSEASMHVHHRWAQAMTLIQIAIALAAIT
                     LLTRSRWLHFVSYGAGGVSVAIGVLALMHV"
     gene            complement(17547..18044)
                     /locus_tag="VFO53_14100"
     CDS             complement(17547..18044)
                     /locus_tag="VFO53_14100"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET8877456.1"
                     /translation="MRLALIAAVAANGVIGRDGTLPWRLPEDLRRFRALTTGHAIIMG
                     RRTWESLPHALPGRQNIVVTRRKGYLAAGASAAASIDAALRAVAYPPPAFCIGGGELY
                     RAALPLAATAFVTEIARAFDGDATFPALDASEWSETAREPHVQEGETGFEYAFVTYER
                     IVRRL"
     gene            18177..19649
                     /locus_tag="VFO53_14105"
     CDS             18177..19649
                     /locus_tag="VFO53_14105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8877457.1"
                     /translation="MYYPRSFLKFILLGFLLVSLPLVYALAEMILSLDRLESQGRQEV
                     VQAAQAGRTSRLLYEQATTLERIARQHLILEDSALLDEYARLREEFRQTTNQLAALPL
                     EPGSQETLEKLADRESRLHKLLTAPRRGSEAADELADGYSKLVDGAQSMLAASNQLTQ
                     RAIERLQETATQGREKWIYVGLATVGIAIALAIVFATLIARPIRQLDLAIRQMGSADF
                     THAIEVTGPQDLRYVGQRLEWLRGRLSELEEQQARFLRHVSHELKTPLTAVREGAELL
                     RDDVGGKLTPEQRDIVRIVRDNTLSLQKLIEDLLAYQQTRAMEPAALRPVALPDVVAR
                     VVREQRLAAVARMITFDAKIEPATVIGDAEKLRTIIDNLLSNALKYSPRNATVRLNVT
                     TNDGFAVLDVVDEGRGVALADRKRIFESFYQGPPPVDGRIKGSGLGLAIAREYALAQG
                     GSIEVAGRADGKSGAWFRLSLPLAPVETARRRQSLQAAHS"
     gene            19657..20385
                     /locus_tag="VFO53_14110"
     CDS             19657..20385
                     /locus_tag="VFO53_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877458.1"
                     /translation="MRGSRACVALALVMALGACASLPTPVMPADENDPATYAVPDPPM
                     VESPPMEPVEPPLPAPPAAAAPANSSPPSSTPPVSASALPPVIVVAPPSPATPPTEDV
                     QMIELLADLQRFGSMQNDELKRELAAASQTFARQRTDINRVRLAVLYTLVRSSPQDDQ
                     RALQLFENVAKSAPASSPIRHLAAVLQAQVNERQRAVHDEQQKADAAIQKLEALRAME
                     RSLLRDRVRSGGGGGASGAGGSGR"
     gene            20424..21779
                     /locus_tag="VFO53_14115"
     CDS             20424..21779
                     /locus_tag="VFO53_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET8877459.1"
                     /translation="MNRGDILLVDDDPDLLKLISLRLTSAGYRVRTADSGETALATIA
                     VERPATVITDLRMPGIDGLQLFEAIHRQHPALPVIILTAHGTIPDAVNATQRGVFGYL
                     TKPFDSQDLLKKVAGALEVSGGEPPGSESTAGQWRANIITRSPRMEDLLRQAKLVADS
                     DASVLVFGESGTGKELLARAIHDASRRSDKPFVAVNCGAIPGDLLESELFGHARGAFT
                     GAIQAHKGLFQAADGGTLFLDEIGDMPLPLQVKLLRVLQEGEVRPVGSTQAIPVDVRV
                     ISATHRDLDSQREQGLFREDLYYRLNVVSLKLPSLAERREDIPLLATHFLRRLAERYR
                     KPTPSLAPDAMALLVAAPWPGNVRQLLNLLEQSVALATTPVIPATLVQAALKEDAAAL
                     VPFEEARKTFEREYLVRLLKITGGNVTQAAQLAKRNRTEFYKLLQRHRLEPGMFKEAK
                     T"
     gene            22057..22209
                     /locus_tag="VFO53_14120"
     CDS             22057..22209
                     /locus_tag="VFO53_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877460.1"
                     /translation="METQAPDLVQHYSASTTSAAEKSRRDDAAVADEAELEQLAERVC
                     AARSVY"
     gene            22370..23341
                     /locus_tag="VFO53_14125"
     CDS             22370..23341
                     /locus_tag="VFO53_14125"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET8877461.1"
                     /translation="MQPFLSLVRSILDTGTWQENRTGIRSISIPGASMRFDLAEGFPA
                     ITTRRLPFRSAVGELIGFLRGARSAAQFRALGCKVWDQNANENAAWLANPYRVGPDWL
                     GEIYGYQWRAWPAYKLLDADNGAQVDDAMARGYRLIGPVDDDGTQKLLLHKAIDQVRG
                     CLDTIIANPADRRILFHAWNCAELDQMALPPCHLLYQLLPNPVTRELSLCLYVRSNDI
                     GLGAPFNVCEAALLLSLVARLTGYAARWLTYFIGDAHIYENQLEMLQTQLSRTPYPPP
                     RLAISERIPALAKTGRYEPEWLERVEPADFSLENYRHHAPLTAPMAV"
     gene            23687..24094
                     /locus_tag="VFO53_14130"
     CDS             23687..24094
                     /locus_tag="VFO53_14130"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET8877462.1"
                     /translation="MIDRNELFLSTHDAATLSAVLATLSPFEAPMPGAADELADMLSG
                     ATLVAPHALGSDVVALNTEVTYVELPRGHARTVQLVHPAAADASAARISVFAPVARAL
                     LGRRAGARVPVALPDAAIDELHVVAVRKERSKS"
     gene            24091..24672
                     /locus_tag="VFO53_14135"
     CDS             24091..24672
                     /locus_tag="VFO53_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877463.1"
                     /translation="MTARLLSRLAARAEFRRGAAMLHPECPIEQTVRGRAAPVQAQLL
                     QEIWRRHSFIADGALLVAEPNCLRVLARTERALAAPVEALRQRYGAEVVVEPASVRYA
                     HGAPVLEPFMNVLVTGLARHLPSVQSDLGQRRGTMTRVDQRKDVFVIEAEAPLGHLLG
                     YADRLDEHFDGGVEASIWLSRYCPIAGDGPYAA"
     gene            complement(24713..25159)
                     /locus_tag="VFO53_14140"
     CDS             complement(24713..25159)
                     /locus_tag="VFO53_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877464.1"
                     /translation="MRAGGTPGGGESETELRRDIGSPRSRRSAQHQRARFFLQRTIGT
                     LPFAFAGTARRKRSHQRAQCVALAPRRLELGADLPVLDGMHEFDPAILADADARLRRF
                     DPAAADALPRRYRLDRKIGLTRPLHIHTGRLADPVPDAIDRAQLNA"
     gene            25143..26312
                     /locus_tag="VFO53_14145"
     CDS             25143..26312
                     /locus_tag="VFO53_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HET8877465.1"
                     /translation="MPPARIAHADFALWNLGFRPFYLLAGFFASVSILLWIAEYAGGL
                     PGGYLHDPVWHAHEMLFGYTIAVVTGFLFTAVRNWTGRPTPSGGLLAAYALLWIAGRI
                     SVLTPYALVSAVLNAVFPIAVAAGIAVPLVRSGNRRNYFFVGLLVLLGLATFAVHMAR
                     LGVVAWPARASLQVALDAVLFIIAVMAGRVVPMFTNNGVPGTRAVRHPLVEKAALGGV
                     LLLILADLSQVDPAWIGVVAIVVALAHAARLWLWQPWRTRSTPLVCVLHVAYAWIVVY
                     LVLRALAAWGVVASPLAVHALTIGTIGGMTIGMMTRTARGHSGRPLVADGVEVTCYAL
                     IVLAAVIRVFGGIALPEFYRATVVLSGLCWSAGFALFTFRYWPILTRPRLDGKPG"
     gene            26364..28271
                     /locus_tag="VFO53_14150"
     CDS             26364..28271
                     /locus_tag="VFO53_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8877466.1"
                     /translation="MSAGKQIAGRSASQYALLRERRFAPFFWTQFLGAANDNVFKNAF
                     VVFVTFEAAGHLAIDAGMIVNLIGAVFILPFMLFSATAGQLADKVEKSRLIRLVKLLE
                     IAIMTIGLAGFVMSSIALLFVALALLGIHSTLFGPVKYAILPQHLAPQELVGGNGLVE
                     AGTFVAILLGTIAGGLVVAIKPGGAIAAGGLGIAIAVAGWLASRRIPYTPAVAPQLVI
                     NPNPVSETWRNMGIARENIVVWRSMLGISWFWFYGAIYLAQLPAFTQRVLGGDEHVFT
                     LLLALFSIGIGIGSLLCERLSGRRVELGLVPFGSIGLSLFAIDLWLATRQMHAVAVDG
                     IGAFVVTVASWRVAIDIVLLGVFGGFYTVPLYALIQERSQPSHRSRIIAANNILNALF
                     IIASAGVAIGLLSAGLSIPQLFLAVGIMNALVAAYIYTLVPEFLMRFLAWLLVHTVYR
                     VHREGLENIPDEGPCVIVCNHVSYVDAVVIAACVRRPIRFVMDHRIFRVPILSFVFRA
                     MRTIPIASARDDPALKDRAFAEAAAALAAGEIVGIFPEGRLTHTGELNRFRAGVRQIV
                     ETTPVPVVPMALRGLWGSFFSRSRQGRAGRSTIGLFKRIALVAAAPVRAEDATPDMLF
                     GQVQALRGDAR"
     gene            28346..31162
                     /locus_tag="VFO53_14155"
     CDS             28346..31162
                     /locus_tag="VFO53_14155"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HET8877467.1"
                     /translation="MPLLLLLAGAMLGALLGRRFEAVASGALIGFIIGYLLESRRKSQ
                     AATRRSDSAGSADPLTLLDPRVAERVRAMERRIAALEAALRDRSSAAGATARPAETGE
                     PASAMRAPVEPVRPQAAAPARDDERIAIDALRADTRASPAPAYGRPRTAPTAPAPRKA
                     SAIRQWIAGGNTLARTGVVVLFIGVAFLLKYASEHVTIPIEARLAGVALGGIALLVFG
                     WRLRARRAGYAVTLQGAGAGILYLTVFAALRLYEVLSPAPAFGLLLAIGALSSFLAIR
                     QDAMALAVLGVLGGFAAPLLASSGGSHVMLFSYYAMLNAAILGIAWFKAWRLLNVVGF
                     ACSVVAGTLWGITTYRPEDFATTEPFLILFFLFYVAIATLYALRRSLELRRYVDATLV
                     FGVPLVAASLQDALVHRIEHALAWSAVGACAIYLLLARLLRARRPELRLLVESFAALS
                     IVFGTLAVPLALDARLTSATWAIEGAAVVWMGVRQGHPSARWFGLLLQLGAGVAFALG
                     FSPWIDPLSGGRLPIANSAYLGAMLVACGGLASSYALARPGAIRPWERVFAPIVFGWG
                     LAWWLGAAWHEVDRFVTADARVAVLVVFLAGSAVLFATLAARLDWPMARVPVYALPGV
                     LFVIALDRVVDPSTRHAHLLAAGGIIAWPLAVAVDVMLLRHLERVTPPSSARGVDAGH
                     AVSVWLVTLIGAAELAWLARTYASGADWPLVAWGLVPALALIAIARLTLSTGWPFGAR
                     AQAYARGGALPVAVAASLWTLVANVSSAGDFAPWPFVPIANPFDVAQALVFVAIARWL
                     RRIHDAELDTPRRPSLDALGVAVAALLLLWVTCSTLRAVHHLTGAPWSLASLWASQVA
                     QSALSLVWSLVALAAMVVANRRGYRIAWVAGAALLGLVVAKLFAVDLAQVGGVERIVS
                     FIGVGVLLLVIGYVAPVPPRAERR"
     gene            31261..32421
                     /gene="aroC"
                     /locus_tag="VFO53_14160"
     CDS             31261..32421
                     /gene="aroC"
                     /locus_tag="VFO53_14160"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET8877468.1"
                     /translation="MSGNTFGKLFCVTSFGESHGPAIGCVVDGCPPGLALAEADIQHD
                     LDRRKPGTSRHVTQRREADSVEILSGVFDGRTTGTPIALLIRNEDARSKDYANIAETF
                     RPGHADYTYWHKYGIRDYRGGGRQSARETAVRVAAGAIARKWLAERRGVAIRGHMTHL
                     GENAIAFEGFEHVDDNPFFAANRSGIPALEAFMDRLRKSGDSCGARIEVIATGVPVGW
                     GEPVYDKLDADLAYAMMSINAVKGVEIGAGFRAVAQRGTQHSDEMAPEGFIGNEAGGI
                     LGGISTGQDVVVAIAVKPTSSIRLDRHSIDKAGRPVIVNTHGRHDPCVGIRATPIAEA
                     MLACVLMDHALRHRAQNADVTVDTPRLAAHAPAEVRQRVRGSAIEDPDPDEA"
     gene            complement(32456..>33117)
                     /locus_tag="VFO53_14165"
     CDS             complement(32456..>33117)
                     /locus_tag="VFO53_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877469.1"
                     /translation="LLFGGGIVKLGDGSIASFLAVAGVFAAAAIVARSGLLAALAVLA
                     LSCCLGARTGYLHAMYFLGIEAPLYTIVLFAALAALLHFVSPRLPTVYHGIASAAVRT
                     SIFLVNFGFWIGSLWGDRSARPIVLGDSVWSALWILAGPRAFAVAWAIVLVAAGIFAW
                     KRDRRWLVSVVAVFGAIDLYTQWFEWLGATPTSVLIAGLAALVLAIALKSFNRKPVAA
                     S"
BASE COUNT         5184 a        11170 c        11294 g         5469 t
ORIGIN      
        1 tagtagatga tgccgttcgc ggcggcgact gcggccagca cgacgacgaa catccacacg
       61 acgtgcaggc cgtagaacca gctcatgctg tcgttcgggc ctcacgccgc acgccgggca
      121 gccttacggg aacgcgggaa tgtgcggatc gatgccgcga tcctcggctt ccggatgctg
      181 cgcgttcagc agcttgcgta tctcgtcgac gcgatgctcg ggcacgtcgg ccatcaacag
      241 cagcctgcct tcgtcgatcg cctgcgagaa ctggcgcagg cgcgaattgg gcaccgcgac
      301 gcccaccatg ctcgcgaccc acgcgccgaa cagcgcgccg atgatgcctg cgccgagcac
      361 cgtgacgatc tgccagcgat cctcggtgac gagcgtcatg acgagcacgc cgcccaggat
      421 gaatccgagc agcgcgccga gtgcgaggcc cacctgcgcg ccgtgcacca ggtcggattt
      481 ctgcagatgg ttcgcctcgt tcagcccgtc catcggcgtg ccgcgcctgg ccaggaagtg
      541 gatgtgacgc gcctcgatcc gcgcgagcag aagatcgtcc atggtctttc gcgcgctcgc
      601 gaggtccggc atgatgaaat agagtctgcg acgcatttca tatcctcctg ccaggacgta
      661 ccgacacact gtacggcggc catgttgcgc tcgcaagccc cacccttttc gccatcggtc
      721 gattgaatgc gcgtcgtcgg catcctgctc gcggccggcc gcggcacgcg cttcggcggc
      781 gacaagctcg cagcgccgct gccgcgggtg tcgcacggcg tgccggcggg gacgccgctc
      841 ggcgtggcgg cggcgatgca tctcgtcgat gcgttgcccg attcggtggc ggtcgtgcgt
      901 cccggcgatc ggctcaccga ggcgctgcgc cgcgccggcc cgcgcatcgt cgaatgcagg
      961 aatgccgacg acggcatggg agcgagcctc gcctgcggtg tggcggcgac gcccgacgcc
     1021 ttgggctgga tcatcgcgct cgccgacatg ccatggattg cgccggcgac gatccgtgca
     1081 gtcgcgaacg cgcttcgtga cggcgcagac atcgtcgcac ccgtccatcg cggcgaacgc
     1141 ggccatcccg tcgccttcgc agcgcgtcac ggcggcgccc ttgccgcctt gagaggcgac
     1201 gccggtgcgc gatcgctgct cgccgcgcat gcgggtgccg tgacattgct cggtgtcgac
     1261 gatgacggtg tcgtgcgcga cgtcgataca cgccaagcgc tcgcagcgcg cgaccgatga
     1321 cccgccttca cgttgcgagc ggatgcagcg tcgcgatgcg ctgcttccac tcgcgcggcc
     1381 cggtctcgtg caccgaggtg ccggtcgaat cgaccgcgac ggtcaccggc atgtcgacga
     1441 cgtcgaactc gtggatggcc tccatgccga gatcggcgaa ggcgaccacg cgcgagcggc
     1501 ggattgcctt cgacacgagg taggcggcgc cgccgacggc aatcaggtac gcggcgcggt
     1561 gccggcgaat cgcgtcgatg gcgacgggtc cgcgctccgc cttgccgatc attgcgatga
     1621 gcccggtctt cgcgagcatc gtctcggtga acttgtccat gcgcgtcgac gtcgtcggac
     1681 cggcgggccc cacggcttca ccccgcaccg cgtcgaccgg cccgacgtag tagatgacac
     1741 gaccggtgaa atcgaccggc agcgcatggc cacgcgcaag catgtcggca atgcgcttgt
     1801 gcgcggcatc gcggccggtg aggatgcgtc ccgacaggag cagcctttcc cccggcgtcc
     1861 agcgcgcgac gtcgtcgcgc gtgagcgtat cgacgttcac ccgccgcgat tgcggcgcgg
     1921 gcgaccattg cacgtcgggc cacgtcgcga gatcgggtgg gtcgagcttc gcgatgccgt
     1981 tgccgtcgag cgtgaagtgc gcgtgccgcg tcgccgcgca attggggatc attgccaccg
     2041 gaaggcttgc cgcgtgcgtc ggatagtcga ggatcttgac gtcgagcacc gtcgacaggc
     2101 cgccgagacc ctgcgcgccc agcccgagcg cgttcacctt gtcgtagagc tcgatgcgca
     2161 gttcgtcgag ccggttcgac ggaccgcgcg ccttcagcgc gtgcatgtcg atcggcgcca
     2221 tcagcgcttc cttcgcgagc agcatcgcct tctccgcgct gccgccgatg ccgatgccca
     2281 gcatgccggg cgggcaccag ccggcgccca tgcccggcac cgactcgacg acccagtcga
     2341 cgatcgagtc ggacggatcg agcatcgcga atctcgcctt gttctccgag ccgccgccct
     2401 tcgcggcgat gcgcacgtcg accgtgtccc ccggcacgat gtcgaagtgc accaccgccg
     2461 gcgtgttgtc gcgggtgttg acgcgcgtcg acgcgggatc gcgcacgatc gacgcgcgca
     2521 gcgtgttgtc gggatcgagg tacgcgcggc gcacgccggc gtcgaccatg tcggccacgc
     2581 tcatcgtcgc gccggcgaag cgcgcgtcca tgccgacgcg cacgaacgcg acgacgatgc
     2641 ccgtgtcctg gcagatcgga cgatgaccct cggcgcacat gcgcgagttg gtgagaatct
     2701 gcgcgatcgc gtccttcgcc gccgccgatt cctcgcgcgc gtacgcgtcg ccgagggcgg
     2761 cgatgtaatc ggccgggtgg tagtaggaga tgaactgcag cgcgtcggcg acgctctgga
     2821 tcaggtcttc ctgcgcgatc tgcgccatga gggaaagtct acctcgtcgc tcgccccgcg
     2881 tcccccgctg gaggtagact tcaaccggaa cagccatcct gccctggagg aattgcgatg
     2941 tccaacatcc ggtccgtgat gaacgaagag cgcgtgttcg agccgccgcg cgggttcgcc
     3001 gcgcaggcga acgtcacgaa ggcgcgcgcc gaagcgatgc gcgcgaaggc cgcccgcgac
     3061 tacgaaggct tctgggccga actcgcgcgc gacacgctta cgtggaagaa gccgttcacg
     3121 aaggtgctcg acgagggcaa ggcgccgttc taccagtggt tcaacgacgg cgagctcaac
     3181 gtctcgtaca actgcctcga tcgcaacctc gagaacggca atgccgggaa gaccgcgatc
     3241 atcttcgagg cggacgacgg tgccgtgaca cgcgtcacgt atcgcgagct gcacgcgcgc
     3301 gtctgccgtc ttgcgaacgg gctgcgctcg ctcggtgtcg cgaagggcga tcgcgtcgtc
     3361 atctacatgc cgatgtccgt cgagggcatc gccgcgatgc aggcctgcgc gcgcatcggc
     3421 gcgacgcact cggtcgtgtt cggcggcttt tcggcgaagt cgctgcacga gcgcatcgtc
     3481 gatgcgggtg cggttgccgt gatcaccgcc gacgagcaga tgcgcggcgg caagccgctg
     3541 ccgctgaagc gtgtcgtcga cgaggcgctc ggcatgggcg gttgcgacgc ggtgcgccac
     3601 gtggtcgtct accggcgcac cggcgccgag gtttcgttcg cggcgggtcg cgacgtgtgg
     3661 atgcacgagc tggccgcgaa gcaggcggac acctgcgaac cggcgtgggt cggcgccgag
     3721 catccgctgt tcatcctgta tacgtcggga tcgacgggca agccgaaggg cgtgcagcac
     3781 agctccgccg gctacctgct gtggtgcgcg ctcacgatgc aatgggtgtt cgacatgaag
     3841 cccgacgacg tcttctggtg cacggccgac atcggctggg tcacgggcca cagctacatc
     3901 acgtacggac cgctcgcatg cggcgcgacc gaggtcgtgt tcgagggcat tcccacgtat
     3961 cccgacgcgg gacgcttctg gaagctgatc gccgatcatc gcgtgagcgt gttctacacg
     4021 gcgccgacgg cgatccgctc gctcatcaag gcgaacaacg tgaatccttc caccgccccg
     4081 tcgaactacg atcttgcgtc gctgcgcctg ctgggctcgg tcggcgagcc gatcaatccc
     4141 gaggcgtgga tgtggtacta cacgaacgtc ggccgcgagc gctgccctgt ggtcgatacg
     4201 tggtggcaga ccgagaccgg cggcctcatg atttcgccgc tgcccggggt caccccgctc
     4261 gtgcccggct cgtgcacggt cccgctgccc ggcatcatgg cggcgatcgt cgacgagacg
     4321 gcccacgccg ttccgaacgg gcagggcggg atgctcgtca tcgagcgtcc gtggccgggg
     4381 atgatccgca cgatctgggg cgatccccag cgctacgtga agtcgtactt ccccgacgag
     4441 ttgggcggca ggctctatct tgccggcgac ggtgcggtgc gcaatgcgaa gaccggctac
     4501 ttcacgatca tgggccgcat cgacgacgtg ctcaacgtct cggggcaccg gctcggaacg
     4561 atggaaatcg aatcggcgct cgtcgccaat ccgatcgttg ccgaggcggc ggtcgttgcg
     4621 cgccccgacg atctgaccgg ggaggcgatc tgcgctttcg tcgtgttgaa gcgcacccgg
     4681 ccgcatgacg gcgaagccgc cgatgtcgcg aaagcgctgc gcgactgggt cggcaaggag
     4741 atcggcccga tcgccaagcc gaaggacatc cggttcggcg acaacctgcc gaagacgcgc
     4801 tcgggaaaga tcatgcggcg gctgttgcgc tcgctcgcac gcggcgagga gatcacccag
     4861 gacgtgtcca cgctcgagaa tccggcgatc ctcgagcagt tgaagcagtc gttgtagggt
     4921 tggaagcccc tcgcgttagg ggttggaagc ccctcgcgct gctcctcgcc ctgcgggcga
     4981 gggcgcgagc ggctctttgt tctatgcgtg tggcggtgcc gtgtcggcga acgacgggcg
     5041 cttgtcgagc ttgtcggcaa gccgcgcgag gttcgggtac agatcgcgcc agtcgagctc
     5101 ggcgaagcga aggtcgaggt agccaagtgc tgcgccggtg gcgatgtcgg cgagcgagta
     5161 gccttcaccg tgacaccatg cacgctcgcc gagctcgcgt gcgagctcgg cggtgccttc
     5221 gtcgatcttg cggcgctgtc gcgcgatcca ttcgtcgctt tgcaggttcg ccggacggcg
     5281 tcgctcgatg acgatcgccg agctcgcatc gcacacgccg tcggccagcg cttcccattt
     5341 cttgacggcg atgcgctggc gcgcgggctc gggaatcaac cgcgacacgg ggctcacgag
     5401 gtcgatgtac tcgacgatga cgcgcgagtc gtaaaggtgc gtgccgtcgt cgaggatcag
     5461 caccggcagc ttgccgagcg gattccatgc gtgcaccgga ttgccgggcg cccatcccga
     5521 cgactcgacc atctggtatt cgatcttctt ttcggcgagc gcgatgcgca ccttgcgcgc
     5581 gtaagggctc gtcgtggagg cgatcagttt cagcacgaaa tggggcgggg agcgctgccg
     5641 gaaggggcag aaattatagc atcgcgtgct aagatcgaac gcttgcctcg cgccatggac
     5701 gccgccgata ccgctcgcct gactgcgctg tcgccgctcg acggccgcta tgcgcagaag
     5761 gtcgacgcgc tgcgcggttg cttcagcgaa ttcgcgctga tccgcgaccg cgtgcgcgtc
     5821 gagatcgcat ggctgctcgc gctgtccgac gagcctgcaa tcggcgaagt tccgccattc
     5881 tccgccgccg cgaaggccgt tcttgccgac atcggtgcgt cgttccgcga agccgacgcc
     5941 gcacgcgtca aggcgatcga acgcacgacc aaccacgacg tcaaggcggt cgagtactgg
     6001 ctgaaggagc gtgtcgcggg cgagccgcaa atcgcgcgcg tcggtgaatt catccacttc
     6061 gcctgcacgt ccgaggacat caacaacctc gcgtacggca tcgcgctggc gcaggcgcgg
     6121 cgggcggtgc tgttgccgac gctcgaggca atcgcaggcg acctgcggca aatggcgact
     6181 gcgtacgccg cctggccgat gctggcgcgc acgcacggtc agcccgccac gccgacgacg
     6241 ctcggcaagg agctcgcgaa cgtctatgcg cgtctcgagc gccagatcgc gacgttcgcc
     6301 aggatcccga tcaagggcaa gatgaatggt gcggtcggca actacaacgc gcacacgctc
     6361 gcttacccgg acgtcgattg ggagcggctc gccgccaaga ccgtgaccgg cctcggcctc
     6421 gaattcagcc cgtacacgac gcagatcgag ccgcacgacg gggttgccga atactgcgat
     6481 gcgctggcgc gaacgaacac gatcctgatc gacctcgacc gcgacgtgtg gggttacgta
     6541 tcgctgggct atttccgcca gcgcacccgc gaaggcgaag tgggctcgtc gacgatgccg
     6601 cacaaggtga atccgatcga tttcgagaat tcggaaggca acctcggcct cgccaatgcg
     6661 ctgttgcgtc accttgccga gaagctgccg gtgtcgcgct ggcagcgcga cctcaccgat
     6721 tcaacggtgc tgcgcaacgt cggcgttgcg ctcggccatg cacagctcgg ctacgtggcg
     6781 ctgcgacagg ggctcgcgaa gctcgatgcc gaccgcgagc ggatggcgaa cgatctcgac
     6841 gcgaactggg aagtgctggc cgaggcgatc cagacgctga tgcgccgcta cggcctgccc
     6901 gagccttacg agcagctgaa ggcgctgacg cgcggcaaga gcggcatgac gcgcgaatcg
     6961 ctgcaggcgt tcatcgacgc gctcgcgctg cccgacgatg cgaaggcgcg tttgcgggcg
     7021 ctcacgccgg cgacgtacgt ggggttggcg gaggagttgg cgaggcgcgt ctagccgcaa
     7081 tcactcgact cgcggcgggc ttgccggtgg tttcgtcgcc gcgcgccgtt ccttcacgaa
     7141 cgtcgtcacc gccggaatca gcgacaccac gacgatcgcg atcacgatga gcgacaggtt
     7201 gtccttgacc cacgggatgt tgccgaacag gaatcccgcg tagacgagaa gcccgatcca
     7261 gaggcagccg ccgatcacgt tgtacgacag gaagcgccgg tactgcatgc ccgccacgcc
     7321 ggcgagaaac ggcgcgaacg tgcggatgat cggcacgaag cggccgatga tgatcgtcac
     7381 ggccccgtac ttttcgtaga acgattgcgt gcggcgcagg tattcctggc ggaaccagcg
     7441 cgagtcgcgc tcgtggaacg ccttcggccc gatgtagtgg ccgatgccgt agttgaccga
     7501 atcgccgagc accgccgccg cgaccagcac ggcgacgagg acgtgcacgt cgaggccggc
     7561 cgctgcgacg acggtgcccg cgatgaacag gatcgagtcg cccggcagga acgggcacac
     7621 gacgagcccc gtctcggcga agatcacgac gaacaggatc gcgtagagcc acgcgccgta
     7681 ctgcgtcgca agcgacgcca cggtctggtc gagcgccagc aacgaataca gaagagtcgt
     7741 cagcatcgcc ggcgcttcag tccgcagcgg cccagagctc ggattggcgg cgcacgacgt
     7801 ccgtcagcgt agcggtctcg tcgaatgccc ggcgcaacca cgcggcgtga ttgccgcgct
     7861 cgaccagctc gccgagcgcc gtgagcgcgg cttgcgagtc gagcatctgt gcatgcggcg
     7921 cgagtgttgc gagcgtttcg gacaaatcct cggcgagcga gcgcgagcgc ccggtcgttg
     7981 cgtcgacgag cgttcccgca aagccgtggc ggcacgcctg gaagcgattg tagctatggg
     8041 gaagatagat atccggcgtg acgtcgaccg gtcgctcctc ccacagccaa cgcgcgagcg
     8101 cctgcgcgaa tgccgcgatc tgcgcggcgc ggtcgaccgt gagcggtgtg tcgcacacgc
     8161 ggatttcgac ggtgccgtat tcgggcttcg gccggatgtc ccagtagaag tccttcatgc
     8221 tcttgacgat cccgagcgcc accatcctgt cgtagtagcg gttgaactcg ctccacgagc
     8281 ggacgagcgg catcgttccc gacagcggaa aggcgttgac gatcgatagc cgcgacgaat
     8341 cgaacgcggt gtcgacgccc tggtagaacg ggctcgaggc ggcgagtgcg atgaagtggg
     8401 gaacgtagcg gatcagtgcg tgcgttaggt agatcgcgtc gtcggcgctt gcgcagccga
     8461 tgtgcacgtg ctggccgaac acggtgaact gcttcgcgag aaagccgtac ttctccgaaa
     8521 cggaaagaaa gcgctcgttc gggtagatct tgcgttcgct ccacttctgg aacgggtgcg
     8581 tgccgccgcc ggcgagggcc acgttcatct tgcgcgcgtg accggcgagc acgtcgcgcg
     8641 tcgagcgcaa ttcggcgagc agttgcggat agcgctcgtg caccgacgtg ttgatctcga
     8701 tcatgctctg cgtgagctcg ggcttgagct cgccctgcgg cgcgtcgcgg gtgatgcgcc
     8761 gcagcaggtc gtcggcgtcg ggcgcgagat tgaagtcgcg cgtgttgacg agcatcagct
     8821 cgagctcgac gccgaacgtc aatgcggagg atgacttgaa cgcgaggtcg gccatatcgg
     8881 ctaatcacgc gcgccgggct cgccttcgcc ggcgcggatg agcgcgtaac gcgtcgcgat
     8941 cggcccgagc aggtggagga tcgcaatgcc cgacacgacg atggccgcga gtcgtgcgcc
     9001 gaattcaggc gcgatgtcgt agatcggctg cgtcatcccg atggcgagcc cggccatcgg
     9061 cgtcagcgcg agcgcaagcg tgcctgcctg gcgcatcgac acgtgtgccg gccaggcgag
     9121 cgcgtacagt ccgaccgcct tgccgagcag gcgcacgacg acgaacccga gcccgatcca
     9181 cgcgacggcg ccgaactggt cgggccgcag cgtggcgccc gtcagcacga acagcgcgac
     9241 gaagaagagc tgggcggcgc ggccgaattc gacgtcgatc agccggtgct tgcgatcgag
     9301 attgcgcgtg caggcgccga gcacgagcag tgcaagcagc accgagagcc ggccgagctg
     9361 cgccgtgccg acggtggcca cgatcatccc gaccgcgagc aggaattgcg gcaccggcga
     9421 cttgccgagc cagcgtgcca acagcagcat cacctggaag gcgaccaagc cgagcaggaa
     9481 cgaccctgcg atcagccata gggcgcggcc aagcggattc agcgtagcgc cggacattcg
     9541 cgcctcgacg aacggcagca gcagcgtgat gccgatagtc gcgatgatgt tgttgagcgc
     9601 cacgtgccac agcatgcggc gagtcaccgg accctcggcc ttgagctcgt tggtgacgag
     9661 cagcacgacc gccggcgccg tggcggtgcc gaccattgcc gcgaccgcgg cctccagcat
     9721 gccgacgccg ccggcgaaat gcagcagcgc gaacatcgcg agaaacgaca gcgcgctctc
     9781 ggcgagaccc atcggcagca gccagcgatc gtgacgcgcc cacgaaagat cgagcctgcg
     9841 cccgaggtcg aagacgatca gcccgagcga gaactcggcg agcacgcgcg agtcgcgaag
     9901 cgccgattcc gagagccagc ccagcacacc gggcccgagc aggaacccgg tggcgatgta
     9961 gccggtgatg gccggaagca cccgcgtcgt cgcggcaagc cgcccgccgg caaggcccgc
    10021 gagcagcagc agcccgaaga tcaggaagct gttggccgcg ggcggccacg cggggaggaa
    10081 atcaggcacg cgctagggga ggatcttctc gcctgccact gcgcgcttgc ggtcggactt
    10141 cacgagatcc tcgcgcgaca cgccgagcca catcacgagc gacgccatga cgagcgcgga
    10201 cgaatagatg ccgaagcaga tgccgatcgc gagggcgagc gagaagtggt gcagcgtctc
    10261 gccgccgaag aagaagatcg acagcaccat cagcagcgtc gacccgtgcg tgatgatcgt
    10321 gcgcgacagc gtgcgcgtga tcgcgctgtc gatcacgtgc gtgacgctcg ccttgcgcat
    10381 cttgcggaag ttctcgcgga tccggtcggc gatgacgacc gattcgttca ccgagtagcc
    10441 gagcacggcg agcacggcgg ccagcacggg cagcgagaac tcccactgga acagcgcgaa
    10501 gaagccgagg atgatcacca cgtcgtgcag gttcgcgatg atcgccgcga cggcgaagcg
    10561 ccactcgaag cgcagcgcga ggtagatcac gatgccgatc gacacgagca acagcgcgag
    10621 cgcgccgttc tcgtagagct ccttgccgac ctgcggcccg acgaattcga cccggcgctg
    10681 cgacgccgtg gggtcgtcgg ccttgagcgc gcccatgacg cgatcggaca gctgtgcgct
    10741 cgaaagcccg gccttcagcg gcaggcgaat gagcgccgtt tcagagctgc cgaagctttg
    10801 caccgagaat tcgccgaggc cgagcttgtc gatggcgctg cgcacgcgcc cgaaatcgac
    10861 cgcgtggcca tagttcacct cgatgacggt gccgccgcgg aagtcgacgc cgagattgag
    10921 cccgtagatc gcgagaaaca cgaccgcggc gacgaacgtg atgatcgaca ccacgttgaa
    10981 cacgagcgcg tgccgcatga acgggatatc gcgcgatatg cggaagaact ccatggtggc
    11041 cttcgggtac gggtgttggc ggcgtcagga cttgaccatc gacgcttcgg gctccgggtg
    11101 ccatgcctgg ccgacggaga tgtggtcgag cttgcgccgg ctgccgtaga tcagcgcgac
    11161 gatgccgcgc gagacgaaga cggcggagaa catcgacgtc aggatgccga gcacgtggac
    11221 gacggcgaag ccgcgcaccg gccccgaccc gaaggcgagg agcgcgatgc ccgcgatcat
    11281 cgtcgtgacg ttcgagtcga ggatggtgcc ccacgcgcgc tcgtagccgg cctggatcgc
    11341 cgcgtgcggt gtcgcgcccc agcgcagctc ctcgcggatg cgctcgttga tcagcacgtt
    11401 ggcatcgatc gccatgccga gcgtcagcgc gatggccgcc atgccgggca gcgtcagcgt
    11461 ggcctgcaga agcgacagga tggcgacgag cagcatcagg ttgatcgcga gtgctgccgt
    11521 cgagatgatc cccatgagca tgtaatagac gcagatgaac gcggcgagga cgaggaagcc
    11581 gtacttgacc gagtcgaagc ccttggcgat gttctccttg ccgagcgacg ggccgaccgt
    11641 gcgttcctcg acgatctcca tcggcgccgc aagggcgccg gcacgcatca aaagcgcgat
    11701 gtcgttcgcc tcgcgcgtgt tcatgcggcc gctgatctgc acgcgcccgc cgccgatttc
    11761 ctcgcggatc accggtgccg tgatcacctc ggccttgccc ttctcgacga gcaggatcgc
    11821 catccgcttg ccgacgttct cgcgcgtgac ttccttgaag atgcgcgcgc cggtgccgtc
    11881 gaggttcacg tggacggccg gctcgttcgt gcgctggtcg aagcccgatt gcgcgtcgct
    11941 gatgcgatcg ccggtgagca cgacctgccg cttgacgaga atgggctcgc cgttgcgatc
    12001 ggtgtagagg tcgttgccga acggcaccga gcccgcgcgc gcggcctcga gtgcgcccgg
    12061 atcgtcgttg accatgcgaa tctcgagtgt cgcggtgcgg ccgaggatgt ccttggcgcg
    12121 ggccgtatcc tgcacgcccg gcagctgcac gacgacgcgg tcggatccct gctgctggat
    12181 gatcggctcg gcgacgccga gctcgttgac gcggttgcgc aggatcgtga tgttctgctg
    12241 gacggcaccc tcgctgatgc gcgtctgcgc ctgcggcttg agcccggcga cgaggcggag
    12301 ctcgccgctt cctgcgtcct gttccttcac gtcgagatcg gcgaacgccg tctcgatttc
    12361 gatccgcgcg cgcgtgcgct cggcggcatc cttgaagcgc agcacgacgt tgctgccttc
    12421 acggccgacg cccgagtaga gcaccttttt ctcgcgcatc agcgaccgga tgtcggtcgt
    12481 gtagcggtcg gccgccttgt cgagcgcgcc cttcatgtcg acctgcagca ggaagtagac
    12541 accaccgcgc agatcgaggc cgagatacat gggcagcgcg ccgatcgacg tcagccacga
    12601 tggcgacgac gagagcaggt tgagcgcgac gatatagttg tcgccgagct tttgcgcgat
    12661 gacgtccttc gcgcgcacct gcgtgtcgag gtcgggaagg cggaccttga tgccgaccgg
    12721 gtcgagctcg gcgccgcggt acggaatgct cgccgacttg agcgcgtcct cgatcgttcc
    12781 gagaagcgcg gtgtcgacct tcacggccga cttcgacgag gagacctgta ccgcaggcac
    12841 gtcgggaaag aagttcggca ccatgtagac gatgccgacc acgagtgcaa tcgcgatgac
    12901 gatgtacttc cagagcgggt agcggttcat tgatttcggc ggcgggggat gaggggcaac
    12961 ggtctcctta gagacccttg agcgtcccct tgggcagcag ctgcgagatc gagccgcgct
    13021 ggaccgtcat ctgcacgttg ggcgcgacct cgaccgttgc atactgctcg tcgagcttga
    13081 cgatgcgtcc gaggatgccg ccggcggtga ccacctcgtt gcccttctcg agcgactcga
    13141 gcatcgcgcg cgcttccttc tgcttcttct gctgcgggcg gatcagcagg aaatagaaca
    13201 cgatgaagat cgcgaccatc ggcaggaagg tcaaaagcgt gttctcttgc gccgcacctg
    13261 cggccgcctg cgcgtaagcg ggggaaatca gcacgaagga gtctccgatt gtggtggcgg
    13321 gttgcgacgt tgcgcccgcg agggtcctgc atgggcgcgc cggcgcggcg ggcgctgacc
    13381 cgtgaccgtc tggttcaacc cgctattcta gcagcgtagc gcgtcgccgc ggcgctcggc
    13441 ttcgaaccgc agcgcgtagt cggcgagcgt tcgcgactcg atcgccgccc ggagctccgc
    13501 catcagcgtc aggtaatagt ggaggttgtg gatcgtcgcg agccgcgcgc cgagaatctc
    13561 gttgatccgc tgcagatggt ggaggtaggc gcgggagaaa tggcggcacg catagcattc
    13621 acacgtgggg tcgagcggcg ccgtgtcgtc gcgatggcgc gcattgcgta tccggatgtc
    13681 gccgtggcgg gtgaaaagcc agccgttgcg cgcgttgcgg gtgggcagca cgcagtcgaa
    13741 catgtccacg ccggcgagca cgccggcaac gatgtcctcc ggcgtgccga cgcccatcag
    13801 gtagcgtggc ttgtcgtctg gaaggcgctg cgccgtatgg cgaagcacgc gcagcatgtc
    13861 gtccttcggc tcgccgaccg acagcccccc gatcgcgtag ccatcgaacc cgatctcgag
    13921 caggccatcg cgcgaacgat cgcgcagatc ctcgtacatt cccccctgga caatgccgaa
    13981 cagcaggttc ggattgccgg cgtgggccgc cttcgagcgt gccgcccagc gcagcgacag
    14041 ctccatcgac accgccgcct cgtcgtgcgc ggccggaaac gccgtgcatt cgtcgaacac
    14101 catcacgatg tccgaatcga ggacacgctg gatctccatc gatatttccg gcgacaggaa
    14161 gaggcggtcg ccattgaccg gcgaagcgaa ggtgacaccg tcctcgtgca ccttgcgcag
    14221 cgccccgagg ctccacacct ggaagccgcc cgagtcggtc aggatcggct tgttccagcc
    14281 ggcgaagcgg tgcagcccgc cgtgtgcgcc gatgacgtcc gtgccgggac gcaaccacag
    14341 atggaacgtg ttgccgagga tgatctgcgc gccgagcgcg gtcagttcgg cgggtgacat
    14401 cgccttgacg gtgccgtacg tgcctaccgg catgaacacc ggcgtttcca cgtcaccgtg
    14461 cgcaagcgtc agccgtgcgc gccgcgccgc gccgcagcgg ccgagaaccg cgaacgacat
    14521 gggcgctgcc tattcggcgg tgatcgtgcc gtcggggtgc gtcgtcagcg gcagtgccgg
    14581 gccgaagccg ccctgcagga acctgccgtc gagaacgatc gcgaacgaga acgccgtgcg
    14641 ccatgcatcg agctcggcac ggctcgccca tccgcggccg atctggccga agtcgcccgg
    14701 cacgatcgcc ggcgcgatct tgcacatggc cgcagcgaag tcgaacgcat tggtccacac
    14761 ctggtagaaa tcgccggcga aatccgttcc gtatgtgccg aacgccggaa gcacgccgta
    14821 ttgcggcgcg atgccgatat ggatcacgca ggcgggaccg gtttgccggt aagcgatggc
    14881 gggatcgacg gcgccgcgcg cgttgggctg cgcccacgcg gccaccacgc tcgttcccgg
    14941 cttcgcctgg ttgccgccgg catacggacc gggattgccg cgcagcgccg cgaggctcgt
    15001 gacgccgcgc gtgagcgggt gcgcaacgat gctcgcgggg gcgagggtgc gcacggcata
    15061 cgcggtgccg acgccgtcgg tcgtgttcgg atcgattccc tcgagagcgc cccagccgtg
    15121 cggggtcgcg gctccggccg tcgcgtcgct gcggtcctga tcgtaaaagg tgccgaggac
    15181 gaccgcacgc ccggcattgg cgaactccgc cgcgcggttg ccgacggccg tcgcgttcac
    15241 gaacacgcga tcctcgaaga gcaggaccac gtcgaaattc gcgaggagca gatcgagcgt
    15301 ggggacgctc gacgtgacgt cgatgccggt gaagacatgg ccgccgatct tcgcgttgta
    15361 atcggccgcc gtttcggcgg accacgcgtt ggacaggatt gcaacgcgcg ctgcgtgagc
    15421 gacggtgcag aagctgaaga cggcaacaac ggccgcgatg atgcggaaca ccgatgacat
    15481 ggaggcgatg catcggccgg cgtgaaatgg cggtcggccg gaaattggga tgtgcgaagc
    15541 gtagccgaga ggcgtcgcgt tgccaatcaa aaccgctgcg caaagcgccg tggccggggt
    15601 tttgtggcat gtgcggcagc gaggcgggca aatgtcgtca gctggcgaca tttctcacgg
    15661 ttccgctatc gcagccgcca gtcggccccc cggagaattc cgcgacgatt tgtcatcgat
    15721 ttccatgctc gagcaacatc gcgtcgccat agctcatgaa gcggtagcgt tgcgaaattg
    15781 cgtgcgcata cgcgccgcgg atcgctgcat ggccgccgaa cgcacttacc agcatcagga
    15841 gtgtcgattt cggcagatgg aagttggtca gcagccggtc gacgacccgg aagcgatacc
    15901 cgggcatgat gaacagcgac gtctgcgacg atcccgcgcg gatcgcgccg tcctcgccgg
    15961 ccgcggcctc cagcgcgcgc aggctcgtcg tgcccacggc aacgatccgc ccgccgcggg
    16021 cgcgggttcc ggcaatcgcg tgcaccgtct ccggagcaat cgaataccac tccgaatgca
    16081 tccgatgctc ggcgatcaga tggctttgca ccggctggaa cgtgccggcg cccacgtgca
    16141 gcgtgaccca ggcagttgcc acgccgcgct ttgccagcgc agcaagcatc gcgcggtcga
    16201 agtggaggcc ggcggtcggt gccgcgactg caccgggatg gcgcgcgtaa accgtctgat
    16261 agcgttcggc gtctgccacg ccgggcgcgt gcgtgatgta cggcggcagc ggcagctcgc
    16321 cgtgctgctc cagccagcgc ggaaggtcgt cgacgccctc gaagcgcagc cgaaagaatc
    16381 ggccgtcgcg cccgatgacg gtggcacgcg cgcccgccgc gagctcgatg gtgccgcccg
    16441 cgcccggcgg atggcttgcc ctcagctgca cgagcgcggc atcgcgctcg cttacccgtt
    16501 cgacgagcat ctcgacgtcg ccgccggtgg gcttgcggcc acggatgcgc gcattgatca
    16561 cgcgcgtatc gttgagcacg aggaggtcgt cgtcgccgac gagtcgcgga aaatcggcga
    16621 agcggaggtc gtcgagcttc gtgccgtcga cgtggagaag gcggctggcc gtgcgctgcg
    16681 gcgccggcga ctgcgcgatc agctccgccg gcagatcgta gtcgaaatcg tcgagcgtcc
    16741 aggggaggtg actgctcacg ccgaagccgc gctccgcggg cccgcgtgcg ttgctggtgc
    16801 cgggagaggg agtcgaaccc tcacgctgtt gccagcggcg gattttgagt ccgctgcgtc
    16861 tgccattccg ccaccccggc cctgcgcgcg ctgcccttgc agtagacgaa cgcgaagacg
    16921 tgcattatcg ccgaatcgcc ctgcagagcc ggtcacacgt gcatcagcgc aagcacgccg
    16981 atcgcgaccg acacgccgcc ggcgccgtac gacacgaaat gcagccagcg gctgcgcgtg
    17041 agcagcgtga tggccgcgag ggcgatcgcg atctggatca gcgtcatcgc ctgtgcccag
    17101 cgatggtgga cgtgcatcga cgcttcgctc ttcgcctcga gttcctccga gcgcttctcg
    17161 agcttctgcg cctgggtcat gatgtcctgc ttttccctgt tgtagcgctc ggcttccgcc
    17221 ttgaatcgtg cgccggcatc gccggtcgcg agcgacgcgc cgagctccgc caggttctgc
    17281 ttgctgctct tcgcctggta gaagttccac tggtcggatg cttcggtccg gcggatcgcc
    17341 gaatcgttct tgaacagcag cgcgtcgttc tgcgtcgcgc cgaccatgta gccgaacagc
    17401 gcgccgatcg tcgcgaagat cgccgtcatc accgcgatcc gcgccgaaaa cgggtcgcgc
    17461 tcgtgcgccg cgtgctcgac ttcgtgatcg tgcgggccat gcacgtggaa ttcatgcgcc
    17521 gacatcgctt cttctcccag gtgttgtcaa agacgccgca cgatgcgctc gtaggtcacg
    17581 aacgcgtact cgaagccggt ctcgccctcc tgcacatgcg gctcgcgcgc cgtttcgctc
    17641 cattcgcttg catccagcgc gggaaacgtc gcgtcgccgt cgaatgcgcg cgcgatctcg
    17701 gtcacgaacg ccgtcgcggc cagcggcagc gccgcacggt acagctcgcc gccgccgatg
    17761 cagaacgcgg gcggaggata cgcgactgcg cgaagcgcag cgtcgatcga cgctgcagcc
    17821 gacgcacccg ccgcgaggta gcccttccgc cgcgtgacga cgatgttctg ccggcccggc
    17881 agcgcgtgcg gcagcgattc ccaggttcgc cggcccatga tgatcgcgtg gcccgtcgtc
    17941 agcgcgcgaa atcgccgcag gtcctcggga aggcgccacg ggagcgtgcc gtcgcgcccg
    18001 atgacgccgt tggccgccac ggcggcgatc agcgcaaggc gcatgcgcgt tgcgcttcgg
    18061 gccggtgcag ggcgcagctt cgatgcgccg gccgggaagc cgaatgcgaa cggcgccgca
    18121 atgtcataat ccgtcgatta tagccagcgc gtcgcccgtg gcgctcgccc caacgcgtgt
    18181 attacccgcg ctcgtttctc aagttcatcc tgctcggctt cctgctcgtc agcctgccgc
    18241 tcgtctacgc gctcgcggag atgatcctct cgctcgaccg cctcgaatcg cagggccgcc
    18301 aggaagtggt gcaggcggcg caggccgggc gcacgagccg gctcctctac gagcaggcaa
    18361 cgacgctcga gcgcatcgcc cggcagcatc tgatcctcga ggacagtgcg ctgctcgacg
    18421 agtacgcgcg cctgcgcgag gagtttcgcc agacgaccaa ccagctcgca gccctcccgc
    18481 tcgagccggg atcgcaggaa acgctcgaga agcttgccga tcgcgaaagc cggctgcaca
    18541 agctcctgac ggcgccgcgg cgcggctccg aagcggccga cgagctcgcc gacggctatt
    18601 caaagctcgt cgacggcgcg cagtcgatgc tcgccgcgag caaccagctc acgcagcgtg
    18661 cgatcgagcg gctgcaggag acggccacgc agggccgcga aaagtggatc tacgtcggtc
    18721 tcgccaccgt cggcatcgcg atcgcgctcg cgatcgtgtt cgccacgctg atcgcgcgac
    18781 cgatccgcca gctcgacctc gcgatccgcc agatgggcag cgccgacttc acgcacgcga
    18841 tcgaggtcac gggcccgcag gacctgcgct atgtcggcca gcgcctggaa tggctccgtg
    18901 ggcgcctgtc cgagctcgag gagcagcagg cgcggttcct gcgacacgtt tcgcacgagc
    18961 tcaagacgcc gctcaccgcc gtgcgcgagg gtgccgagct gttgcgtgac gacgtcggcg
    19021 gcaagctgac ccccgagcag cgcgacatcg ttcgtatcgt gcgcgacaac acgctttcgc
    19081 tgcagaagct gatcgaggac ctgctcgcct accagcagac gcgggcgatg gagccggcgg
    19141 cgcttcgccc cgtggcattg cccgatgtcg tcgcgcgggt cgtgcgcgag cagcggcttg
    19201 ccgccgtcgc gcgaatgatc acgttcgacg cgaagatcga gcccgccacg gtgatcggcg
    19261 acgccgagaa gctgcgcacg atcatcgaca acctgctgtc gaacgcgctc aagtattcgc
    19321 cgaggaatgc gacggtccgg ctgaacgtga ccaccaacga cggcttcgcg gtgctcgatg
    19381 tcgtcgacga gggccgcggc gtcgcgctcg ccgatcgcaa acgcattttc gagtccttct
    19441 accagggccc gccgccggtg gacgggcgca tcaagggctc cgggctcggc cttgccatcg
    19501 cccgcgaata cgcgctcgcg cagggaggca gcatcgaagt ggcgggccgc gccgacggca
    19561 agagcggcgc gtggttcagg ctgtcgctgc cgctcgcgcc ggtcgagacc gctcggcggc
    19621 gccagtcatt gcaggcggca cattcgtgag cacgccatga ggggaagccg ggcctgcgtg
    19681 gcgctggcgc tcgtaatggc actcggcgcc tgcgcgtcgc tgccgacgcc ggtcatgccc
    19741 gcagacgaaa acgatccggc cacctacgcc gttcccgatc cgccgatggt cgagtcgcca
    19801 ccgatggagc cggtcgagcc gccgctgccg gcgccaccgg ccgccgcagc gccggcaaat
    19861 tcgtcgccgc cttcgtcgac gcctcccgtt tccgcttctg cgctgccgcc cgtgatcgtc
    19921 gtcgcaccgc cgtcccccgc aacgccgccg accgaggatg tgcagatgat cgaactgctc
    19981 gccgacctgc agcgcttcgg ctcgatgcag aatgacgagc tgaagcgcga gctcgcggct
    20041 gcgtcgcaga cgttcgcccg ccagcgcacc gacatcaatc gcgtccggct cgccgtgctc
    20101 tacacgctcg tgcgctcgtc gccgcaggac gatcagcgcg cgctgcagct cttcgagaac
    20161 gtcgcgaaaa gcgcgccggc gtcgtcgccg atccgccatc tcgcggccgt cctgcaggca
    20221 caggtgaacg agcggcagcg cgcggttcac gacgagcagc agaaggccga tgcggcgatc
    20281 cagaaactcg aggcgctgcg ggcgatggag cgcagcctcc tgcgcgatcg tgtgcgtagc
    20341 ggcggcggcg gcggcgcatc cggcgccggc ggatccggcc gctgaaaccc gttttataag
    20401 gtgctgcgcg ccgaaaggaa ttcatgaatc gtggagacat cctcctggtc gacgacgacc
    20461 ccgacctgct caagctcatc agcctgcgtc tcacgtcggc gggctatcgc gtgcgcactg
    20521 ccgacagcgg cgagaccgcg cttgcgacga tcgccgtcga acgaccggcg accgtcatca
    20581 ccgacctgcg gatgcccggt atcgacggcc tgcagctctt cgaggccatc catcgccagc
    20641 atccggcact gcccgtcatc atcctcacgg cgcacggaac gatccccgac gccgtcaacg
    20701 cgacgcagcg cggcgtattc ggctacctga cgaagccgtt cgacagccag gacctattga
    20761 aaaaggtggc cggcgccctc gaggtttcgg ggggcgagcc gcccggcagc gaaagcaccg
    20821 ccgggcaatg gcgcgcgaac atcatcacgc gcagcccccg gatggaagac ctgctgcgcc
    20881 aggcgaagct cgtcgccgat tccgacgcca gcgtgctcgt cttcggcgaa tcggggaccg
    20941 gcaaggagct gctcgcccgc gcgatccacg acgcaagccg gcgcagcgac aagccgttcg
    21001 tcgccgtcaa ttgcggcgcc atccccggcg atcttctcga atcggagctg ttcggtcatg
    21061 cgcgcggcgc gttcaccggg gccattcagg cgcacaaggg gttgttccag gccgccgacg
    21121 ggggaacgct gttcctcgac gaaatcggcg acatgccgct gccgctgcag gtgaagctgc
    21181 tgcgcgtgct gcaggaaggc gaggtccgcc ccgtcggctc cacgcaggcg attccggtcg
    21241 acgtccgcgt catctcggca acgcaccgcg acctcgattc gcagcgcgag cagggcctgt
    21301 ttcgcgagga tctctattac cggctgaatg tcgtctcgct caagctgcca tcgctcgccg
    21361 aacggcgcga ggacatcccg ctgctcgcca cgcatttcct gcgccgcctt gccgagcgct
    21421 accgcaagcc gacaccgtcg ctcgcgcccg atgccatggc gttgctggtg gccgccccct
    21481 ggcccggcaa cgtacgccag ctcctgaacc tgctcgagca gtcggtcgcg ctcgcgacga
    21541 cgcccgtcat cccggcgacg ctcgtgcagg cggcgctcaa ggaggatgcg gccgcgctcg
    21601 tacccttcga ggaagcgcgc aagacgttcg agcgcgaata cctggtgcgc ctgttgaaga
    21661 tcaccggcgg caacgtcacg caggcggcgc agcttgccaa gcgcaatcga accgagttct
    21721 acaagctgct gcagcgccac cggctggagc cgggaatgtt caaggaggcg aagacgtagg
    21781 catccattgc cgctgatgca ggctgtcgtt ggtgggcgac acgcaatacc gcattgcttc
    21841 aatgacttgc cgatccacgc tgctttgctg tcggtcgtgc gcgacaaaac gagcccccgc
    21901 aaaggcgctg cggcacctgg acccggtgcc aatgcgccat tcgcaacgac cgctttccct
    21961 atggcgggtg cgtggcgttc aagttgatgg catgtcgatt gctttgaacg gttgcgtaac
    22021 cagcgcgcac ccgcgcaacg taaagcggat cgacacatgg aaacccaggc ccccgacctc
    22081 gttcaacact acagcgcgag caccacgagc gccgccgaga agtcacggcg cgacgatgct
    22141 gcggtcgcgg acgaggcgga gctcgagcag ctcgccgagc gcgtctgcgc ggcgcgcagc
    22201 gtttactgac gcggcacgcg ttcgcttcga gcgcagtacg gcgctgggcc gcgcgctggc
    22261 cgcctcggcc ttcgatcgcg cactgtaatc ggcggacgcc catcggttcg gtggcggtgc
    22321 ggtatcctcg tgcaccggct tcgttttccg cttgccggtc gccacgcgca tgcaaccatt
    22381 cctatcgctc gtacggtcga tcctcgacac gggcacctgg caggaaaacc gcacggggat
    22441 tcgctcgatc agcattcccg gcgcatcgat gcgtttcgac ctcgccgaag gctttccggc
    22501 gatcacgacc cgccggctgc cgttccggtc ggccgtcggc gagctgatcg ggttcttgcg
    22561 cggcgcccgc agtgcggcgc aatttcgcgc actgggctgc aaggtctggg atcagaacgc
    22621 gaacgaaaac gcggcgtggc tcgccaatcc gtatcgggtc ggacccgact ggctcggcga
    22681 gatctatgga taccagtggc gcgcctggcc agcgtacaag ttgctcgatg ccgacaacgg
    22741 cgcgcaggtc gacgacgcca tggcgcgcgg ctaccgcctg atcggccccg tcgacgacga
    22801 cggcacgcaa aagctgctgc tgcacaaggc gatcgaccag gtgcgcggct gcctcgacac
    22861 gatcatcgcc aatcccgccg atcgccggat cctttttcat gcgtggaact gcgccgagct
    22921 cgaccagatg gcgctgcctc cctgtcatct gctgtatcag ctcctgccca atccggtgac
    22981 gcgcgagctg tcgctgtgcc tgtacgtccg ctcgaacgac atcgggctcg gcgcgccatt
    23041 caacgtgtgc gaggcggcgc tgctgctgtc gctcgttgcg cgtctcactg gctacgcggc
    23101 gcgctggctg acgtacttca tcggcgatgc gcacatctac gagaaccagc tcgagatgct
    23161 gcaaacgcag ctttcgcgta cgccgtatcc gccgccgcgc ctggcgattt ccgagcgcat
    23221 tccggcgctt gcaaagaccg gacgttatga gcccgaatgg ctcgagcgcg tcgaaccagc
    23281 ggatttttca cttgaaaatt accggcacca cgcgcccctg accgccccga tggcggtatg
    23341 aatgcgcaac gtccatcgtc ttgcattcgt gggaatattt gcataaatcg ccggatgttg
    23401 ctatcatccg gaaatgcgag cgatttataa cttcttgaag tgccaccacc ggcgtcccgt
    23461 gccggtggcc gccggcgctc gcctcgccct tcgccgagct tccgcctcgc tcgcgggccg
    23521 gccctcgatc caccgcaacg accgcggcac gctcgcctgt ctcgggtgag cccggcaagc
    23581 cgcgggcggc gcttttcggg ccgtcggtgc ttgccgggag cgacaaccat ccagccgttg
    23641 cgccgcaagg cgccgtctgc ctgtccttgc tctgtccgga ggtcccgtga tcgatcgcaa
    23701 cgaactcttc ctgtccacgc atgacgcggc aacgctgtcc gccgtgctcg ccacgctgtc
    23761 acctttcgag gcgccgatgc ccggcgctgc cgacgaactt gccgacatgc tgtcgggtgc
    23821 aacgctcgtt gccccgcacg cgcttggctc cgacgtggtc gccctcaaca ccgaggtgac
    23881 gtacgtcgag ctgccgcgcg gccacgcgcg cacggtgcaa ctcgtccatc cagcggccgc
    23941 cgatgcgtcg gcggcacgca tttcggtgtt cgcacccgta gcgcgcgcgc tgctcggccg
    24001 ccgtgcgggc gctcgcgtcc cggtggcgct gcccgacgca gccatcgacg agctgcacgt
    24061 cgtcgccgtc cgcaaggaaa ggagcaagtc atgacggccc ggctgctgtc gcgtctcgcg
    24121 gcgcgcgccg agtttcgccg cggtgctgcg atgctgcatc ccgagtgccc gatcgagcag
    24181 acggttcgcg ggcgcgccgc gccggtgcag gcacagctgc tgcaggaaat ctggcggcgc
    24241 cactcgttca tcgcggacgg cgcgctgctc gtcgccgagc ccaactgcct gcgcgtgctc
    24301 gcccgtaccg agcgcgcgct cgcagcgccg gtcgaggcgc tgcgccagcg ctacggggcg
    24361 gaggtcgtcg tcgagcccgc gtcggtgcgt tatgcccatg gggcaccggt cctcgagccg
    24421 ttcatgaacg tgctcgtgac cgggctcgcg cgtcacctgc catcggtgca gagcgatctc
    24481 gggcagcgcc gtgggacgat gacgcgtgtc gatcagcgca aggacgtctt cgtcatcgag
    24541 gccgaggcgc cgctcggcca tctgctcggc tacgccgacc ggctggacga gcacttcgat
    24601 ggcggcgtgg aagcgagcat ctggttgtcg cgttattgcc cgatcgcagg tgacggtccg
    24661 tacgcggcat gacgcttgcg gcggcgcgct ttggcgcagc gcgccgcccg tttcacgcgt
    24721 tgagttgcgc gcgatcaatg gcgtcgggca ccggatcggc taatctcccc gtatggatgt
    24781 gcagcggacg agtcagcccg attttccggt cgagacgata tcggcgtggc aatgcatcgg
    24841 ctgcggcagg atcgaagcgc cgcagacgtg catcggcgtc tgccaggatc gccgggtcga
    24901 attcgtgcat gccgtcgagc acaggcaagt cagcgccgag ctcgaggcga cgcggcgcga
    24961 gcgcgacgca ctgcgcgcgc tggtgcgaac gcttgcgtcg agccgtcccc gcgaaggcga
    25021 atgggagcgt tcctatcgtg cgctgcagga aaaagcgcgc gcgctgatgt tgcgcgctgc
    25081 gccgggaacg gggcgagccg atgtcgcggc gtaactcggt ttcgctctca ccgcccccgg
    25141 gcgtgccgcc ggcccgcatt gcgcacgccg acttcgcgtt gtggaatctc ggcttccggc
    25201 cgttctacct gctcgcgggg ttcttcgcat cggtctcgat cctgttgtgg atcgccgaat
    25261 atgccggcgg cctgcccggg ggctacctgc acgatcccgt ctggcatgcg cacgaaatgc
    25321 tgttcggcta cacgatcgcc gtcgtcaccg gcttcctttt caccgccgtg cgtaactgga
    25381 ccggccggcc gacgccctcc ggcgggctgc tcgcggcgta cgcgctgctc tggatcgccg
    25441 gccggatcag cgtgctgacg ccgtacgcgc tcgtgtcggc ggtgctcaac gccgtgttcc
    25501 cgatcgccgt cgccgcgggc atcgcggtac cgctcgtgcg cagcggcaac cggcgcaatt
    25561 atttcttcgt cgggctgctc gtgctgctcg gcctcgcaac gtttgccgtg cacatggcgc
    25621 gcctcggcgt cgtggcctgg cccgcacgcg cgagcctgca ggtcgcgctc gacgcggtcc
    25681 tgttcatcat cgcggtgatg gccggacgcg tcgtgccgat gttcacgaac aacggcgttc
    25741 ccggtacgcg tgccgtgcgc catccgctgg tcgagaaggc ggcgctcggc ggcgtgttgc
    25801 tgctgatcct ggccgatctg tcgcaggtcg atccggcgtg gatcggcgtc gtcgcgatcg
    25861 tcgttgccct agcccacgcg gcgcggctgt ggctctggca accgtggcgc acgcgctcga
    25921 ccccgctcgt gtgcgtgttg cacgtcgcgt acgcctggat cgtcgtctac ctcgtgctgc
    25981 gcgcgctcgc cgcatggggc gtcgttgcgt cgccgctcgc cgtccatgca ttgacgatcg
    26041 gcaccatcgg cggcatgacg atcggaatga tgacgcgcac ggcgcgcggt cactcggggc
    26101 gcccgctcgt cgccgacggc gtcgaggtga cctgctatgc gctgatcgtg cttgccgccg
    26161 tgattcgcgt gttcggcggc atcgcgttgc ccgagttcta ccgcgcaacg gtcgtgctgt
    26221 ccggtctctg ctggtcggcc ggcttcgcgc tcttcacctt tcgctactgg ccgatcctga
    26281 cgcgcccgcg cctcgacggc aaacccgggt agcgcacttt cgcttgcgcg gacctgcccg
    26341 cgccgcgaga atgcgcggca ctgatgagtg caggtaaaca aatcgccggg cgatccgcga
    26401 gccagtacgc gctgttgcgc gagcgccgct tcgcgccgtt cttctggacc cagttcctgg
    26461 gcgccgcgaa cgacaacgtc ttcaagaacg cgttcgtcgt gttcgtcacg ttcgaagcgg
    26521 caggtcatct cgccatcgat gccgggatga tcgtgaacct gatcggcgcc gtgttcatcc
    26581 tgccgttcat gctgttttcg gcgaccgccg gccagctcgc cgacaaggtc gagaaatcgc
    26641 ggctgatccg cctcgtgaag cttctcgaga tcgcgatcat gacgatcggc ctcgccggct
    26701 tcgtcatgtc gagcatcgcg ctgctcttcg tggcgctcgc gctgctcggc attcattcga
    26761 cgctgttcgg tccggtcaag tacgcgatcc ttccgcagca tctcgcgccg caggagctcg
    26821 ttggcggcaa cgggctcgtc gaagcgggaa cgttcgtcgc gatcctgctc ggcacgatag
    26881 cgggcgggct cgtcgtcgcg atcaagcccg gcggcgcaat cgccgccggc gggctcggga
    26941 ttgccatcgc ggtcgccggc tggcttgcga gccggcgcat cccgtacacg ccggccgttg
    27001 cgccgcagct cgtgatcaat ccgaatccgg tcagcgagac gtggcgcaac atggggatcg
    27061 cgcgcgagaa catcgtcgtc tggcgctcga tgctcgggat ctcgtggttc tggttctacg
    27121 gcgcgatcta ccttgcgcag ctgcccgctt tcacgcagcg ggtgctgggc ggcgacgagc
    27181 acgtgttcac gctgctgctc gcgctgttct cgatcgggat cggcatcgga tcgctgctgt
    27241 gcgagcggct gtcggggcgc cgcgtcgagc tcggcctcgt gccgttcgga tcgatcggat
    27301 tgtcgctgtt cgcaatcgac ctgtggctcg cgacgcggca gatgcacgcg gtcgcggtgg
    27361 acggcatcgg cgcattcgtc gtgacagtgg ccagctggcg cgttgcgatc gacatcgtgc
    27421 tgctcggcgt cttcggcggc ttttacacgg tgccgctcta tgcgctgatc caggagcgat
    27481 cgcagccgtc gcatcggtcg cgcatcatcg cggcgaacaa tatcctgaac gcgctgttca
    27541 tcatcgcgtc cgccggcgtc gccatcggcc tgctttccgc ggggctgtcg ataccgcaac
    27601 tgttcctcgc cgtcggcatc atgaatgcgc tggtcgccgc ctacatctac acgctggtgc
    27661 ccgagttcct gatgcgtttt ctcgcgtggc tgctcgtgca cacggtttac cgcgtgcacc
    27721 gcgaaggcct ggagaacatt cccgacgagg gcccgtgcgt catcgtctgc aaccatgtga
    27781 gctatgtcga cgcggtggtg atcgccgcgt gcgtacgccg gccgatccgc ttcgtcatgg
    27841 atcaccggat tttccgcgtg ccgatcctgt cgttcgtctt ccgcgcaatg cgcacgatcc
    27901 cgatcgcgtc ggcgcgcgac gacccggcgc tgaaggatcg cgcgttcgcc gaggccgcgg
    27961 cagcactcgc ggcgggcgag atcgtcggca tcttccccga gggacggctc acgcataccg
    28021 gcgagctcaa ccggtttcgg gccggtgtcc ggcagatcgt cgagacgacg cctgttccgg
    28081 tcgttccgat ggcgctgcgc ggcctgtggg gaagcttctt ttcgcgatcg cgacagggca
    28141 gggcgggacg cagcacgatc ggtcttttca aacgcatcgc gctggtggcg gccgcgccgg
    28201 tgcgcgccga ggacgcgacg cccgacatgt tgttcggaca ggtgcaggca ttgcgcggcg
    28261 acgcgcgctg agcggaaccc gaacgcccgt accatgagct gacgccgcgc cggtcatcgg
    28321 tggcgcgacg ctgggagaac gcttcatgcc gctgttgctg ttgctcgctg gcgcgatgct
    28381 cggggcgctg ctcgggcgtc gattcgaagc ggtcgcatcg ggcgcgctga tcggcttcat
    28441 catcggctac ctgctcgagt cgcggcggaa gtcgcaggcg gcaacccggc gatcggacag
    28501 tgcgggttcg gccgatccgt tgacgctgct cgatccccgc gttgccgagc gcgtgcgcgc
    28561 gatggagcgg cgcattgcgg cgctcgaggc ggcactgcgc gacaggagtt ccgccgccgg
    28621 agccacggcg cggcctgcgg aaaccggcga gccggcgtcc gcgatgcgcg cgcccgtcga
    28681 gccggtccgt ccgcaggcgg cagccccggc cagagacgac gagcgcatcg cgatcgacgc
    28741 gcttcgcgcc gatacgcggg cgagccctgc acctgcctat gggcggccac gcacggctcc
    28801 cacggcgccg gcgccacgga aggcaagcgc gatacgccag tggatcgccg gcggcaatac
    28861 gcttgcgcgc accggcgtcg tcgtgctgtt catcggcgtc gcgttcctgc tgaaatatgc
    28921 ttccgagcac gtgacgattc cgatcgaggc acgtcttgcc ggcgtggcac tcggcggcat
    28981 cgcgctgctc gtgttcggct ggcggctgcg cgcgcggcgt gccggttacg ccgtgacgtt
    29041 gcagggcgcc ggtgccggca tcctgtacct caccgtattc gcggcgctgc ggctctacga
    29101 ggtgctgtcg ccggcccccg cgttcggatt gctgctcgcc atcggcgcat tgtcgagctt
    29161 cctcgcgatc cgccaggatg cgatggcgct cgccgtgctc ggcgtgctcg gcggattcgc
    29221 cgcgccgctg ctggcgtcga gcggcggcag ccacgtgatg ctgttttcgt attacgccat
    29281 gctgaacgcg gcaatcctcg ggatcgcgtg gttcaaggcg tggcggctgc tgaacgtcgt
    29341 cggcttcgcc tgcagcgtcg tcgcgggaac gctgtggggc atcaccacgt accgccccga
    29401 ggatttcgca acgaccgagc cgttcctgat cctgttcttc ctgttctacg ttgccattgc
    29461 gacgctgtac gcactgcgac gctcgctcga gctgcggcga tacgtcgacg caacgctcgt
    29521 gttcggcgtg ccgctggttg ctgcaagcct gcaggacgcg ctcgtgcacc gcatcgagca
    29581 cgcactcgca tggagcgcgg tcggcgcgtg cgcgatctac ctgctgctcg cgcgcctgct
    29641 ccgcgcgcgc cggcccgagc tgcggctcct ggtcgaatcc ttcgccgcac tgtcgatcgt
    29701 gttcggcacg cttgcggtgc cgctcgcgct cgatgcacgc cttacatctg ccacctgggc
    29761 gatcgagggc gcagccgtcg tctggatggg cgtgcgccag gggcatccgt ccgcacgctg
    29821 gttcggcctg ctgctgcagc ttggcgccgg cgttgcattc gcactcggct tttcgccgtg
    29881 gatcgatccg ctatccggcg gacgattgcc gattgcgaac agcgcgtacc tgggcgccat
    29941 gctcgtcgcg tgcggcggtc tcgcgagttc gtacgcgctg gcgcggcccg gcgcaatcag
    30001 gccgtgggag cgcgttttcg ccccaattgt gttcggatgg ggactcgcgt ggtggcttgg
    30061 cgcggcatgg cacgaggtgg atcgttttgt caccgccgat gcgcgcgttg ccgtgctcgt
    30121 cgtctttctc gcgggctcgg ccgtgctgtt cgccacgctc gcggcaaggc tcgactggcc
    30181 gatggcgcgc gttcccgtgt acgcactgcc tggcgtgctg ttcgtgatcg cgctcgatcg
    30241 cgtcgtcgat ccttcgacgc gccacgcgca tctgctcgct gccggcggca tcattgcatg
    30301 gccgctcgcc gtggccgtgg acgtcatgtt gctgcgccac cttgaacggg tgacgccgcc
    30361 atccagcgcg cgcggcgtcg acgccggaca cgcggtcagc gtgtggctcg tgacgctgat
    30421 cggcgccgcg gagctcgcgt ggcttgcacg cacgtatgcg agcggtgccg actggccgct
    30481 cgtcgcatgg ggcctcgttc cggcgctggc gctgattgcg attgcgcggc tcacgttgtc
    30541 caccgggtgg ccgttcggcg cgcgggcgca ggcgtacgct cgcggcggtg cgcttcccgt
    30601 cgccgtggcc gcatcgctgt ggacgctggt cgcgaacgtc tcgagtgccg gagacttcgc
    30661 accctggccg ttcgtgccga tcgccaatcc gttcgacgtc gcgcaggcac tcgtcttcgt
    30721 tgcgatcgcg cgttggctgc gccgcattca cgacgccgaa ctcgacaccc cgcgccgccc
    30781 gtcgctggac gcgctcggcg tcgccgttgc cgcgctgctg ctgttgtggg tcacgtgctc
    30841 gacccttcgc gccgtgcatc acctgaccgg cgcgccgtgg tcgcttgcgt cgctgtgggc
    30901 gtcgcaggtc gcgcagtcgg cgctgtcgct cgtctggtcg ctggttgcgc tcgcggcgat
    30961 ggtcgtcgcc aatcggcgcg gctatcgcat cgcctgggtt gcaggtgctg cactgctcgg
    31021 tctcgtcgtg gcaaagctct tcgccgtcga cctggcgcag gtcggtggcg tcgagcggat
    31081 cgtgtcgttc atcggcgtcg gcgtcctgct gctcgtgatc ggctacgtgg cgccggttcc
    31141 gccgcgcgca gagcgccgat gaaaccggcc acgccgaagg acgcgcaccg gtgacgaagc
    31201 gtgcgcggat cggccgcgcc tcgatgctat agtcatccgg gcgaaagcga ccggcgcacc
    31261 atgtcaggca acaccttcgg caagctcttc tgcgtgacgt cgttcggcga gtcgcacggg
    31321 ccggcgatcg gctgcgtcgt cgacggctgt ccgccgggcc tcgcactcgc cgaagccgac
    31381 atccagcacg atctcgaccg ccgcaagccg ggcacgtcgc ggcacgtgac gcagcggcgc
    31441 gaagcggaca gcgtggagat tctctccggc gtgttcgacg gccgcaccac cggcacgccg
    31501 atcgcgctcc tgattcgcaa cgaggacgcg cgcagcaagg attacgcgaa tatcgcggag
    31561 acgtttcgcc ccggtcacgc cgactacacg tactggcaca agtacggcat ccgcgattac
    31621 cgcggcggcg gccgccagtc ggcgcgcgaa acggcggtgc gcgttgccgc cggcgcgatc
    31681 gcacgcaaat ggctcgccga gcgccgcggc gtcgccattc gcggccacat gacgcatctt
    31741 ggcgagaacg cgattgcgtt cgagggcttc gagcacgtcg acgacaatcc tttcttcgcg
    31801 gcgaaccggt cgggcattcc ggccctcgag gcgttcatgg accggctgcg caaatcgggc
    31861 gattcctgcg gcgcgcgcat cgaggtgatc gcgacgggcg ttccggtcgg ctggggcgag
    31921 ccggtctacg acaagctcga tgcggacctt gcctacgcca tgatgagcat caacgcggtg
    31981 aagggcgtcg agatcggcgc cgggtttcgc gcggtcgcgc agcgcggcac gcagcattcg
    32041 gatgaaatgg cgcccgaggg tttcatcggt aatgaagctg gcggcattct cggcggaatc
    32101 tccaccggcc aggatgtcgt cgttgcgatc gcggtcaagc cgacgtcgtc gatccggctc
    32161 gaccggcact cgatcgacaa ggccggccgg ccggtcatcg tgaacacgca cggtcgccac
    32221 gatccttgcg tcggcatccg cgcgaccccg atcgccgaag cgatgctcgc gtgcgtgctg
    32281 atggaccatg cgctgcgcca tcgcgcccag aacgccgatg tcaccgtcga cacgccgcgg
    32341 ctcgccgcgc acgcgccggc ggaggtccgc cagcgcgtcc ggggcagcgc gatcgaggac
    32401 cccgatccgg acgaggcgta ggaccgagcg gcgccgcccg gcggcgtcgt gcgcttcagg
    32461 acgccgcgac cggcttgcga ttgaacgact tgagcgcaat ggccagcacc agcgccgcga
    32521 gtccggcgat cagcacgctc gtcggcgtcg cgcccagcca ctcgaaccat tgcgtgtaca
    32581 ggtcgatcgc gccgaacacc gcgacgacgc tcacgagcca gcggcggtcg cgcttccacg
    32641 cgaagatgcc ggccgcgacg agcacgatcg cccaggccac cgcaaaggcc cgcggccccg
    32701 cgagtatcca aagcgcggac cacacgctgt cgccgagaac gatcgggcgt gcgcttcgat
    32761 cgccccacaa cgatccgatc cagaagccga agttgacgag gaagatcgac gtccgcacgg
    32821 ccgccgacgc aatgccgtgg tagacggtcg gcaggcgtgg cgaaacgaag tgcagcaatg
    32881 ccgcgagtgc cgcgaacagc acgatcgtgt agagcggcgc ctcgatgccg aggaaataca
    32941 tcgcgtgcag gtagccggtg cgcgcgccga gacagcacga cagtgcgagc acggcgagtg
    33001 cggccagcag cccgctccgc gccacgatcg cggcggcggc aaagacgccg gcgacggcga
    33061 ggaacgacgc gatcgacccg tcgccgagct tgacgatgcc gccgccgaac aacagcg
//
LOCUS       DASSVX010000329         4752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1331677, whole genome shotgun sequence.
ACCESSION   DASSVX010000329 DASSVX010000000
VERSION     DASSVX010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331677"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VFO53_14170"
     CDS             complement(<1..834)
                     /locus_tag="VFO53_14170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8877470.1"
                     /translation="MNRAERRRSKERRTGRANAAPALAHAIRRHRAGDLDAAEAAYAE
                     RLRHHPEDPDALHFLGVLRHQQQRPDEAIALVTRALEIAPGHVDAWNNLGNICKQSGR
                     LEQAEDAYRRALSLDARHAGAWNNLGVVLGALRRGTESIAALEEAIRCSPRMVAAYFN
                     LGGAYRGCGTLREAIGAYCKVLELDPEHGRAHEMLGRVLYLAGEHAAAASIFRAWADV
                     DPDNPVPVHMLAACSGENVPVRACDAYVRATFDAFADSFDEMLLQRLDYHAPQRLLDA
                     LA"
     gene            1027..1617
                     /locus_tag="VFO53_14175"
     CDS             1027..1617
                     /locus_tag="VFO53_14175"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HET8877471.1"
                     /translation="MYRTNGKRKDRRALMAAALSAVALAAGNPNVAVAASDIFVKLGD
                     IKGESIDSKHKDEIELLSWSWGVVGSFRNSAKQPQQPQQPAASAQAACAQDITIVKTV
                     DSASPLLFAKAASGANISSATFSVRKSDTSQDYLVFTLSDVMVTSVSQGGAEGGGTPT
                     ESVTFGFSGGTIAVMRQSATGAAAEPIVASVPASCR"
     gene            complement(1804..4362)
                     /gene="mutS"
                     /locus_tag="VFO53_14180"
     CDS             complement(1804..4362)
                     /gene="mutS"
                     /locus_tag="VFO53_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET8877472.1"
                     /translation="MMQQYLRFKNEHPERLLFYRMGDFYELFYADAERASQLLDITLT
                     ARGQSAGAPIPMAGVPYHAIEQHLARLVARGESAVIVDQVGDPAASKGLVERRITRIV
                     TPGTLTDAGLLDAKRDAPLAALVQDGERAGIAWLNLASGRMTLTDVALVDCAATLERI
                     EPAELLLAEDAVAPAWRSKGAAVHALPAWHFDRGTAARALARQMGTVDLRAFGAASAP
                     LATGAAGALLAYASATQQSTLEHVRSLDVEAASEFVSLDAATRRNLEITQTLAGEREP
                     TLHSLLDTCATAAGSRLLRHWLTHPLRDQARVAARHDAIDALRADPSSMRSITAALRG
                     TIDVERVVSRIALRSARPRDLGGLRDTLASLPALARIGAASDALLLRDCAHDLACDPQ
                     WHALLARAIAPEPAAQLRDGNVIADGFDAELDELRNVDAGCAQFLLDLERRERERSGI
                     ATLKVEYNRVHGFYIEVTRANAERVPDDYRRRQTLKNAERYTTAELTAFESKALTAQE
                     RALARERRLYDELLAALAPAIAQLQGAAMALATLDVLATLAERADALRLVRPQLRPAI
                     GIAIEGGRHLVVERQVDAFIPNDVALDPARRLLVVTGPNMGGKSTYMRQTAVIALLAY
                     AGMFVPAARAALGPLDAIYTRIGAADDLAGGRSTFMVEMTEAAYILNRATAQSLVLID
                     EIGRGTSTFDGLALAWSIAHRLAAHNRSLALFATHYFELTALPSESDGCANVHFDAVE
                     TKSRQGPGIVFLHQVEDGPANRSYGLQVARLAGVPADTLKLAQRYFARLDKFNARDDA
                     QHDLFTAADAAQPPAAASGLRLAELEAKLAALDPDNLSPRDALAALYELKRSVR"
BASE COUNT          753 a         1568 c         1651 g          780 t
ORIGIN      
        1 cgccagcgcg tcgagcaagc gctgcggcgc atggtagtcg agccgctgca gcagcatctc
       61 gtcgaagctg tccgcgaagg catcgaacgt ggcccgcacg taggcgtcgc acgcgcgcac
      121 gggcacgttc tccccggagc aggcggcaag catgtgcacc ggcaccggat tgtccggatc
      181 gacgtcggcc catgcgcgga agatcgacgc agcggccgca tgctcacccg caaggtacag
      241 cacgcggccg agcatctcgt gcgcgcgccc gtgttccggg tcgagctcga gcaccttgca
      301 atacgcgccg atcgcctcgc gcagcgtccc gcagccgcga tacgcgccgc cgaggttgaa
      361 gtaagcagcc accattcgcg gcgagcagcg gatcgcttcc tcaagcgcgg cgatcgactc
      421 cgtgccgcga cgcaacgcac ccagcacgac ccccaggttg ttccacgcac ccgcgtgtcg
      481 ggcatcgagc gaaagcgctc gccgatacgc gtcctcggcc tgctcgagac ggcccgattg
      541 cttgcatata ttgccgaggt tgttccacgc gtcgacatgg ccgggcgcga tctcgagcgc
      601 gcgcgtcacg agcgcgatcg cctcgtccgg tcgttgctgc tgatgacgaa gtacgccgag
      661 gaaatgcagc gcgtccgggt cctcgggatg gtgacggagc cgttcggcat acgccgcctc
      721 ggcggcgtcg agatcgcccg cgcggtggcg gcggatcgcg tgagccagcg cgggcgcagc
      781 gttcgctcgc ccggtccggc gttccttcga gcggcgacgc tcggcgcgat tcatcctcgg
      841 ttcccggcgc ggcacagggc gaccgcgcgt cgccccgaat tgtcggcgaa gccggccagg
      901 gccacaatcc acgcgcccgg gtttcaggaa actaagcgct cgctgtgaat tcccgcggcg
      961 ggcagcgcca gagggcccgc ggaattgtac cgagacaagc gacgttcaac cacgagggag
     1021 aatcgaatgt atcgtacaaa cggtaagcga aaggaccgcc gcgcgttgat ggcggcggcg
     1081 ctgtcggctg tcgcacttgc agcgggaaat ccgaacgtgg cggtcgccgc gtcggacatc
     1141 ttcgtaaagc tcggcgacat caagggcgag tcgatcgaca gcaagcacaa ggacgagatc
     1201 gagttgcttt cgtggtcgtg gggcgtcgtc gggtcgtttc gcaacagcgc aaagcagccg
     1261 cagcagccgc aacagccggc cgcctcggcg caggccgcct gcgcgcagga catcacgatc
     1321 gtgaaaaccg tcgacagcgc atccccgctg ctgttcgcga aagcggcgtc cggcgccaac
     1381 atttcgtcgg ccacgttctc cgtcaggaag agcgatacct cgcaggacta tctcgtgttc
     1441 acgctctcgg acgtgatggt cacgtcggta tcgcaggggg gtgccgaagg cgggggaaca
     1501 ccgacggagt cggtgacctt cggcttttcc ggcgggacga tcgctgtcat gcgtcaatcg
     1561 gcgacggggg ccgctgccga gccgatcgtc gcctcggtgc cggcgtcgtg ccgctaggcc
     1621 agggagtcgg gcgcgcgagc gcgtccttcg actagcgcgc tcaactccag ggaaacgggc
     1681 tcgcgcgaac gagcagcaga tcgagcgctg cgagctcgtc gataccgcag tgcgcggcgc
     1741 cgccgacctc gccggcgcct tgtgcagacg ggagacagtg acccgcggcg gcatgcgcgg
     1801 atgttagcgc accgaccgct tcagttcata aagcgctgcg agcgcgtcgc gcggcgacag
     1861 gttgtcgggg tcgagtgctg cgagcttcgc ttccagctcg gcgaggcgca gtccgcttgc
     1921 cgctgccggc ggctgcgccg catccgccgc ggtgaacagg tcgtgctgcg catcgtcgcg
     1981 cgcgttgaac ttgtcgagcc gcgcgaagta gcgctgcgcc agtttgagcg tatcggccgg
     2041 cacgccggcg agccgcgcga cctgcagccc gtagctgcgg ttcgcggggc cgtcctcgac
     2101 ctggtgcagg aacacgatgc ccggtccctg ccggctcttc gtctcgaccg catcgaagtg
     2161 cacgttcgcg cagccgtcgc tttccgacgg cagcgccgtc aactcgaagt agtgcgtcgc
     2221 gaaaagcgcg aggctgcggt tgtgcgccgc gagccgatgc gcgatcgacc acgcgagcgc
     2281 caggccgtcg aacgtcgacg tgccgcggcc gatctcgtcg atcagcacga ggctttgcgc
     2341 cgtcgcgcgg ttcaggatgt acgcggcctc ggtcatctcg accatgaacg tcgagcgccc
     2401 gccggccagg tcgtcggcgg cgccgatgcg cgtgtagatc gcgtcgagcg gcccgagtgc
     2461 cgcgcgcgcg gcgggcacga acatccccgc gtaggcgaga agcgcaatca ccgccgtctg
     2521 ccgcatgtac gtcgacttgc cgcccatgtt gggtccggtg acgaccagaa gccgccgcgc
     2581 cgggtcgagc gcaacgtcgt tcggaatgaa cgcgtcgacc tggcgctcga cgaccagatg
     2641 ccgcccgcct tcgattgcga tgccgattgc ggggcgcaat tgcggacgca cgagtcgcaa
     2701 tgcgtcggcg cgttcggcaa gtgttgcgag gacgtcgagc gtcgcgagcg ccatggcggc
     2761 gccttgcagt tgcgcaatcg ccggcgcaag cgctgcgagc agctcgtcgt agaggcgccg
     2821 ctcgcgggca agggcgcgct cctgcgcggt gagcgccttc gattcgaacg ccgtcagctc
     2881 ggcggtcgta tagcgttcgg cattcttgag cgtctgccgg cgccggtaat cgtcgggcac
     2941 gcgctcggcg ttcgcgcgcg tgacctcgat gtagaagccg tgcacgcggt tgtattcgac
     3001 cttcagcgtg gcgatgcccg agcgctcgcg ctcgcgccgc tcgaggtcga gcaggaactg
     3061 cgcgcacccg gcatcgacgt tgcgcagctc gtcgagctcg gcatcgaatc cgtcggcaat
     3121 gacattgccg tcgcgcaact gcgccgccgg ctcgggcgcg atcgcacgcg cgagcagcgc
     3181 atgccattgc ggatcgcacg cgagatcgtg cgcgcagtcg cgcaacagca acgcatcgga
     3241 cgccgcgccg attcgcgcca gtgcgggaag cgatgcaagc gtgtcgcgca gacctccgag
     3301 atcgcgcggc cgcgcgctgc gcagcgcgat gcgcgacacg acgcgctcga cgtcaatcgt
     3361 gccgcgcagc gccgcagtga tcgagcgcat gctagaaggg tcggcgcgca atgcatcgat
     3421 cgcgtcgtga cgcgccgcaa cgcgcgcctg gtcgcgcagc ggatgcgtga gccaatggcg
     3481 cagcagccgg cttccggccg ccgttgcgca cgtgtcgagc agcgagtgca gcgtcggctc
     3541 gcgctcgccg gcgagcgttt gcgtgatctc gaggttgcgg cgggtcgcag cgtcgagcga
     3601 cacgaattcg ctcgccgctt cgacgtcgag cgagcgcacg tgctcgagcg tgctctgctg
     3661 cgtcgcgctc gcatacgcga gcagcgcgcc cgcggcgccg gtcgcgagcg gtgccgaggc
     3721 ggcaccgaag gcgcgcaggt cgacggtccc catctgtcgt gccagtgcgc gcgcggccgt
     3781 gccccggtcg aaatgccatg cgggcaacgc atgcaccgcc gcccccttgc tgcgccaggc
     3841 gggcgcgacg gcgtcctcgg cgagcagcag ctcggcgggc tcgatgcgtt cgagcgttgc
     3901 ggcgcagtcg acgagtgcga cgtcggtcag cgtcatccgc cccgaagcga gattgagcca
     3961 ggcgataccg gcgcgctcgc catcctgcac cagcgcggcg agcggcgcgt cgcgctttgc
     4021 gtcgagcagc ccggcgtcgg tcagcgtgcc cggcgtgacg atccgcgtga tcctgcgctc
     4081 gacgagcccc ttgcttgccg ccggatcgcc gacctggtcg acgatcaccg cactttcgcc
     4141 acgcgcgacg agtcgcgcga gatgctgctc gattgcgtgg tacggcacgc ccgccatggg
     4201 aatcggcgca cccgccgact ggccccgggc ggtgagcgtg atgtcgagga gctgcgaggc
     4261 acgctcggcg tcggcgtaga acagctcgta gaaatcaccc atccggtaga agagcagccg
     4321 ctcggggtgc tcgttcttga accgcaggta ctgctgcatc atcggcgtgt gggcggcgtg
     4381 tgtgcttgta tcgttatttt tcacttaata atcaatatgt tattgttttt ttgttttgct
     4441 tgatgtggta attttggggt aatttttcgc gctctgaggt ggcttcgggc ggttcggtgc
     4501 acgggcaaaa ttcgctgaac agtgaatttt gtaacctagg cgaatattcg gtatagtgac
     4561 gttattcgct gccaggagaa taacacctgc atgaccgtgt cgcccactgc ggaaagcgtt
     4621 cttatcaagc agtttctgga ttcgctccgg gaattgccgg acgtgcatgc cgaattggaa
     4681 gggccggaat tcaccacggc tgcggatgac cgtctcgacg ccaagattca cctggatatc
     4741 gccggcaagt cg
//
LOCUS       DASSVX010000330         9632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1098194, whole genome shotgun sequence.
ACCESSION   DASSVX010000330 DASSVX010000000
VERSION     DASSVX010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098194"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..444)
                     /locus_tag="VFO53_14185"
     CDS             complement(28..444)
                     /locus_tag="VFO53_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8877473.1"
                     /translation="MTLKSLLLGSAFAALAAVNLASAQQVPAATSQSAPIKRTPLQKF
                     DVPGTSYETVIGMAEIVPNVNIGRHTHPGLESGYVLEGDMVLIVQGQPDKTLRTGESY
                     QIPLGAVHDARSGPKGAKVIATYVVEKGKPLATPAP"
     gene            complement(574..1617)
                     /locus_tag="VFO53_14190"
     CDS             complement(574..1617)
                     /locus_tag="VFO53_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HET8877474.1"
                     /translation="MTLHISIRPSGHTFGAEADETVLQAAMRADLMIPYGCRNGACGT
                     CKGRILAGDVDFGAHQPSTLTDDEKRKGLALFCVARPKTDIVIEVREVRRAGDIQIKR
                     LPCRIESIDKPAPDVAIVKLKLPVNERLQYLAGQYIDFLLRDGHRRSFSIATPPHADE
                     LLELHIRHTSGGFFTDPLFTTYKGREILRFEGPLGTFYLRETSDKPVIFVAGGTGFAP
                     IKAIIEHMLHHRIPREIVLYWGVRAKADLYMPHVPGKWQQENANFTFIPVLSDPAPGD
                     AWPGRTGFVHRAVIDDFRDLSGYQIYACGAPAMIDAAKRDFTTLRGLPEEEFFADSFT
                     YAAETETTALAPG"
     gene            1616..2524
                     /locus_tag="VFO53_14195"
     CDS             1616..2524
                     /locus_tag="VFO53_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET8877475.1"
                     /translation="MMQRLLLVGCGDIALRVAGLLRGRVRLLGLTRNRDDVRKLRSRG
                     IVPLFGDLDDRRSLARLDLGTSAVLHCVPPPAEGRDDPRTRNLLAALSGARIIPRHFV
                     YLSTSGVYGDCAGARVAETRPVRAQTPRARRRAAAERRLRAWAGHNGVTLSILRVPGI
                     YAPTRLPLERLRHGTPALVADEDVYTNHIHADDLAHAAVAALYFGRANRAYNVCDDAE
                     MKMGAWFDTVADAFHLPRPARVSWDDAERRIAPMLLSFMSESRRLANARMKRELRVRL
                     RHPTPYAMLVQCAPRELRRQLSLPIG"
     gene            2524..3459
                     /gene="ubiA"
                     /locus_tag="VFO53_14200"
     CDS             2524..3459
                     /gene="ubiA"
                     /locus_tag="VFO53_14200"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HET8877476.1"
                     /translation="MAGAPLIPRPGAPRRKLTLVERLDAYERLMRLDKPIGTLLLLWP
                     TLCALWLAARGEPRGSIVLIFAVGTLVMRSAGCAMNDWADRDFDAHVARTASRPLAQG
                     IIAPWEALVVAAVGAFVAFLLVLFTNRATMLWSIPALGIAIVYPFVKRFFAIPQAFLG
                     IAFSFGIPMAYAAVQARVPPLAGALLVVNLFWVVAYDTEYAMVDRADDAKIGIRTSAL
                     TFGRADVFMVGACYALYLIGMVFIGRIYALGAIYYAALAIALLIAVHHLRIIRSRDPA
                     ACFRAFLGNHWLGMAVFAGIAVDYAYRARQWPRVW"
     gene            3525..4007
                     /locus_tag="VFO53_14205"
     CDS             3525..4007
                     /locus_tag="VFO53_14205"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HET8877477.1"
                     /translation="MRGLPPVIAADARVLILGSFPSEASLAAAQYYAHPRNHFWPILG
                     DVLREPLAQLPYAERLARVKAHRVAIWDTIVACERSGSLDAAIRNAEQGEIHRVHRLA
                     RELRAVCFNGKTAARARPAWAEAGYATLVMPSTSPAYTLPIAEKLVLWRELARFLEAR
                     "
     gene            4004..4885
                     /locus_tag="VFO53_14210"
     CDS             4004..4885
                     /locus_tag="VFO53_14210"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET8877478.1"
                     /translation="MKRLAVALLCAGVALGAQARRVESVTFPSLDRDGNGATVLLHAM
                     LLLPAGPARGARPAIVALHGCGGMYSTREGHEHELAERLALRADPLLRDGYAVLFVDS
                     FRSRGLAQICTIRHADRTVKVAQRRLDALGALAYLAHRGDVAGERVALLGWSHGGSVA
                     LQAIEKQNRAAGAAFFRGAVAFYPGCASALALGSRYRPGAPTRVYIGELDDWTPAATC
                     VELGKAMAARNEDLRVATYPDSYHGFDAPRGQLVHRIDVPNGVHPGEGVHVGPNAAAR
                     EAANASVRAFLRERLRD"
     gene            complement(4892..5122)
                     /locus_tag="VFO53_14215"
     CDS             complement(4892..5122)
                     /locus_tag="VFO53_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877479.1"
                     /translation="MGLRIIIVAAVLALLSGCAPQPPQADAEWRPEKIYRTGSNIPAK
                     DYGAENIEVGKPGAADSMIRPGTGVLGRKPGG"
     gene            5276..8065
                     /locus_tag="VFO53_14220"
     CDS             5276..8065
                     /locus_tag="VFO53_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8877480.1"
                     /translation="MNDARSAAVIPLRPSAADAHADLVAFLFTDIEGSSLKWLNHRAA
                     MQEALRVHDEILRKAVVAHGGEVFKTGGDAFSAAFRRPSDAVGAAIDAQQALARADFG
                     SVDGLAVRMAVHVGTAERREGDYFGPALNRVARLLSLGHGGQVLVTSSASELIAAERE
                     NRHTLRHLGAHPLDDPLQPVGVHQVDVPGLPHEFPPLRTTENRPTNLPRQPTPLIGRD
                     AELERVAALLSQNSLVTVTGTGGVGKTRITLEAGTRLLERFPDGVWLVELAAIFDATL
                     VASAATNALAVDTSSAKTPQEALLSRLKHQHLLLLLDNCEHVIEAVAALVESLLAVAP
                     NVCVLVSSQEPLGIAGEQVFRLPSLAVPKDAQVRSEDAMRAGAVQLFVERAKGADPRF
                     ALDDRNAATVAAICRRLDGIPLAIEMAAARAPMLGVDKLAQKLDERFRVLTGGRRTAL
                     PRQQTLRATLDWSYGLLSERERTVLRRLSIFAGGFTLDGAGRVASDDAIDEFEVIDLL
                     SHLVARSLVVADTDEGGGTRYRLLETTRAYALEKLADARESPAIERLHATFFRDLFER
                     AYDDWQTTPDVEWRNAYLPERDNLRNALDWAFGPSGDTAIGIEIAGVTSEMWWQLSLT
                     AEGRQRMEAMAPHVDETLPPHVAGRFWLAFGRFWANIAPLRGAEARARAAALFRDANL
                     PAKRAEALALYAQGLPETSELDAKRQAALAEAWPLVEQAALPRLLGQYFNTSARFHII
                     SNPALARDHFESALRHYRHAGAEAQVLNTLNNLADFTWSTGDLSAAVASMREAVVLVR
                     GSTVSDRTLLGQVLGNLAGALVEQGEIDEALVTLREAIPIIREGGWFFRFGDHLALRL
                     AKAGHADAAARLLGYADAEHARFEANRQVNEARAHESVLAILNAAIAPAELAQRMAEG
                     AKLSEDEAARLALEP"
     gene            8070..9602
                     /gene="ubiD"
                     /locus_tag="VFO53_14225"
     CDS             8070..9602
                     /gene="ubiD"
                     /locus_tag="VFO53_14225"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HET8877481.1"
                     /translation="MAYRDLRDFIAQLEAAEDLKRVAAPVDPNLEMTALCDRALRAQG
                     PALLFEHPKGDRRGPSGNAIPVLGNLFGTPERVAKAMGADAANWQKSLRETGELLSFL
                     REPAPPKSLVDAWRNTRPVLMKVLDMTPKLRHGGPCQEVLWEGDEVDLARLPIQTCWP
                     GDAGPLITWGLTVTRGPHKKRQNLGIYRQQVIARNKVIMRWLAHRGGALDFRDHAIAA
                     PGTAFPIAVALGADPATILGAVTPVPDTLSEYQFAGLLRGGRTEIVKCLTHDLQVPAS
                     AEIVLEGFIHPDANDPSGYETALEGPFGDHTGYYNETERFPVFTIDRMTLRRDPIYHS
                     TYTGKPPDEPAMLGVALNEVFVPLLQKQYPEISDFYLPPEGCSYRLAVVAMKKQYPGH
                     AKRVMFGIWSFLRQFMYTKMIVVTDDDVDIRDWKDVIWALTTRVDPARDTLIVPNTPI
                     DYLDFASPVAGLGSKIGIDATNKWPAETARQWGRPIAMDADVTARIEAIWRTISTSGS
                     EL"
BASE COUNT         1434 a         3270 c         3279 g         1649 t
ORIGIN      
        1 gcctggcggt ctcgccgatc gcgtgcctca gggcgcaggc gtcgccaacg gcttgccctt
       61 ttcgaccacg taggtcgcaa tgaccttggc ccctttcgga cccgacctcg catcgtgtac
      121 cgcgcccaac gggatttgat agctttcgcc cgttcgaagc gtcttgtccg gctgaccctg
      181 aacgatcaac accatgtcgc cttccaggac gtagcccgat tcgagtcccg gatgcgtgtg
      241 acggccgatg ttcacattcg gcacgatctc ggccatcccg atcacggtct cgtagctcgt
      301 ccccggcacg tcgaatttct gcagtggcgt gcgcttgatc ggcgcgctct ggctggtggc
      361 ggcaggcact tgctgtgcgc tcgccaggtt gacagccgcg agggcggcga atgcgcttcc
      421 gagcagaagt gatttcagcg tcatgttttc cccttggtct ttacacgagc ggcgtgattg
      481 cggtcgtgcg tcgcaagctt actgccaggg atcggccagc ggcacaatgc ctgtcagggg
      541 gtagaggtcg aggcgctcgc cgcgcgcccg acgctatccg ggcgccagcg cggtcgtttc
      601 cgtctcggcg gcgtacgtga agctgtccgc gaaaaactcc tcctcgggaa gcccgcgcag
      661 cgtcgtgaaa tcgcgcttcg ccgcgtcgat catcgccggc gcgccgcacg cgtagatctg
      721 gtagcccgac aggtccctga agtcgtcgat caccgcgcgg tgcacgaatc ccgtgcgtcc
      781 cggccatgca tcgcccggcg cggggtccga cagcacggga atgaacgtga agttcgcatt
      841 ctcctgctgc cacttccccg gcacgtgggg catgtagaga tctgccttcg cccgcacgcc
      901 ccagtagagc acgatctcgc gcggaatccg gtgatgcagc atgtgctcga tgatcgcctt
      961 gatcggcgcg aaccccgtgc cgccggcgac gaagatcacc ggcttgtcgc tcgtctcgcg
     1021 caggtagaac gtgccgagcg ggccttcgaa gcgcaggatc tcgcggccct tgtacgtggt
     1081 gaacagcgga tcggtgaaga agccgccgct cgtgtggcga atgtgcagtt cgagcagttc
     1141 gtcggcgtgc ggcggcgtgg cgatcgagaa gctgcgccga tgcccgtccc gcaacaggaa
     1201 atcgatgtac tggccggcaa gatattgcag ccgctcgttg accggcagct tgagcttcac
     1261 gatcgcgacg tccggcgccg gcttgtcgat ggattcgatc cggcacggca gccgcttgat
     1321 ctggatgtcg ccggcgcggc gcacctcgcg cacctcgatg acgatatcgg ttttcggacg
     1381 ggcgacgcag aagagggcga ggcccttgcg cttttcgtcg tccgtcagcg tcgacggctg
     1441 gtgcgcgccg aaatcgacgt cgcccgccag gatgcgcccc ttgcacgtgc cgcacgcgcc
     1501 gttgcggcaa ccgtagggga tcatcaggtc ggcgcgcatc gccgcctgca gcaccgtctc
     1561 gtcggcctcg gcgccgaacg tgtgcccgct cggccttatg ctaatgtgca gggtcatgat
     1621 gcagcggctg ttgctcgtgg gttgcggcga tatcgcgttg cgcgtcgccg gcctgttgcg
     1681 gggccgggtg cgccttctcg gcctcacgcg caaccgcgac gacgtacgca agctcagaag
     1741 ccgcgggatc gtgccgctgt tcggcgacct cgacgatcgc cgttcgctcg cgcggctcga
     1801 tctcgggaca tcggcggttc tgcactgcgt gccgccgccg gccgaaggcc gcgacgaccc
     1861 gcgcacgcgc aacctgctcg cggcactttc gggcgcgcgg attataccgc gtcacttcgt
     1921 ttatctctcg acgtcgggcg tctatggcga ctgcgccggc gcgcgcgtcg cggaaacgcg
     1981 cccggtgcgt gcgcagacgc cgcgcgctcg ccgtcgcgcg gccgccgagc gccggcttcg
     2041 cgcatgggcg ggccacaacg gggtgacgct atcgatcctg cgcgttcccg gcatctacgc
     2101 gccgacccgg ttgccgctcg agcggttgag gcacggcacg ccggcgctcg tcgcggacga
     2161 ggacgtgtac acgaatcaca tccacgccga cgatcttgcg cacgcagcgg ttgccgccct
     2221 gtatttcggc cgcgcgaatc gtgcgtacaa cgtgtgcgac gacgccgaga tgaaaatggg
     2281 cgcgtggttc gacacggtgg ccgacgcatt tcacctgccg cggccggcac gcgtgtcgtg
     2341 ggacgacgcc gagcgccgta ttgcgccgat gctgctgtcg ttcatgagcg agtcgcggcg
     2401 gcttgcgaac gcgcgcatga agcgcgaatt gcgcgtgcgg ctgcgccatc cgacgccgta
     2461 cgcgatgctt gtgcaatgcg cgccgcgcga gttgcgccgc cagctttcgc tgccgatcgg
     2521 ctgatggcgg gggcgccgct catcccacgt ccgggtgcgc cccggcgcaa gctcacgctc
     2581 gtcgagcggc tcgacgcgta cgagcgcctg atgcgcctcg acaagccgat cggcacgctg
     2641 ctgctgctgt ggccgacgct ttgcgcgctg tggctcgccg cgcgcggcga gccgcgcggc
     2701 tcgatcgtgc tgattttcgc ggtcggcacg ctggtcatgc gctcggccgg ctgcgcgatg
     2761 aacgactggg ccgaccgcga cttcgatgcc cacgtcgcac gcacggcgag ccggccgctt
     2821 gcacagggaa tcattgcgcc ctgggaagcg ctcgtcgtgg cggccgtcgg cgcgttcgtc
     2881 gccttcctgc tcgtgctgtt caccaatcgc gcgacgatgc tgtggtcgat cccggcgctt
     2941 ggcatcgcaa tcgtctatcc gttcgtcaag cgcttcttcg cgatcccgca ggcgttcctc
     3001 ggcatcgcgt tctcgttcgg catcccgatg gcgtatgccg ccgtgcaggc tcgcgtgccg
     3061 ccgctcgcgg gggcgctcct cgtcgtcaac ctgttctggg tcgtcgccta cgacaccgag
     3121 tacgcgatgg tcgaccgtgc cgacgacgcg aagatcggca tccgcacctc cgcgttgacg
     3181 ttcggccgtg cggacgtttt catggtgggt gcctgctacg cgctctacct gatcggcatg
     3241 gttttcatcg gccgcattta cgcgctcggc gcgatctatt atgcggcgct cgcgatcgcg
     3301 ctgctgatcg cggtccatca cctgcggatc atccgcagcc gcgatcccgc ggcctgcttt
     3361 cgcgcgtttc tcggcaacca ctggctgggc atggcggtgt tcgcgggcat cgccgtcgac
     3421 tacgcgtatc gcgcacgaca gtggccgcgc gtttggtgac gaagcgcgtt gcgtccccgt
     3481 ccggggcaac gcaaccagac cggcgcaagc atcgcgacgc gccgttgcgc ggcctgcctc
     3541 cggtcatcgc cgccgatgcg cgcgtgctga tccttggaag ctttccgagc gaagcgtcgc
     3601 ttgcggcggc gcagtactac gcgcatccgc gcaaccattt ctggccgata ctcggcgacg
     3661 tgctgcgcga gccgcttgcg caactgccat acgccgagcg tctggcgcgc gtgaaggcgc
     3721 atcgcgtggc gatctgggac acgatcgtcg cctgcgagcg cagcggcagt ctcgatgcgg
     3781 cgatccgcaa tgccgagcag ggcgagattc atcgcgtgca ccggctcgcc cgcgagttgc
     3841 gtgcggtgtg cttcaatggc aagaccgcgg cgcgcgcgcg ccctgcatgg gccgaggcgg
     3901 gctacgcgac gctcgtcatg ccgtcgacca gccccgcata tacgctcccc atcgccgaga
     3961 agctcgtgct gtggcgcgag ctcgcccgtt tcctggaggc gcggtgaaac gtctggcggt
     4021 tgccttgctg tgcgccggcg ttgcgctcgg cgcgcaggcg cgccgggtcg agtccgtcac
     4081 gtttccatcg ctcgatcgcg acgggaacgg tgcgaccgtt ctgctgcatg ccatgctgct
     4141 gctgcccgcc ggacctgcac gaggcgcccg gccggcgatc gtcgcgctgc acggatgcgg
     4201 cggcatgtat tcgacgcgcg aaggacacga gcacgagctc gccgagcggc ttgcgcttcg
     4261 cgccgaccca ctgctgcgcg atggttacgc cgtgctgttc gtcgacagct tccgctcgcg
     4321 cggcctcgcg cagatctgca cgatcaggca cgccgaccgg accgtcaagg tcgcgcagcg
     4381 gcgcctcgat gcgcttggcg cactggccta cctcgcccat cgcggcgacg ttgcgggcga
     4441 gcgcgttgcg ttgctcggct ggtcgcacgg cggcagcgtc gcgctgcagg cgatcgagaa
     4501 gcaaaatcgc gcggccggcg cagctttctt tcgcggcgcc gtcgcgttct atccgggatg
     4561 cgcgagcgcg ctcgcgctcg ggagccgcta tcggccgggc gcgccgacac gcgtctacat
     4621 cggcgagctc gacgactgga cgccggccgc gacgtgcgtc gagctcggca aggcgatggc
     4681 ggcccgcaac gaagacctgc gcgtggcgac gtatccggac agctaccacg ggttcgacgc
     4741 accgcggggc caactcgtgc atcgaatcga tgtgccgaac ggcgtgcatc cgggcgaggg
     4801 cgtgcacgtg ggacccaatg ccgcggcgcg cgaagccgcc aacgccagcg tgcgcgcgtt
     4861 cctgcgcgag cggctgcgcg actgatcgag atcaaccgcc cggcttgcgt cccagcacgc
     4921 ccgttcccgg ccggatcatc gagtcggcgg cgcccggctt tccgacctcg atgttctcgg
     4981 ccccgtaatc cttggccgga atgttgctgc ccgtgcgata gattttttcc ggtcgccatt
     5041 cggcgtccgc ctgcggcggt tgcggggcgc aaccggacag caaagcgagc acggcagcga
     5101 caatgatgat gcgcaagccc atggtttttt cctcccgttc aacgacggat tatccaacac
     5161 aaccgaatgc cgcttcccga cttttcgacg taaccccgaa ttcgaaccat ggaacccgcc
     5221 tattgcgcga cgaacgcggc ttgctagact ggcggcgccc tggaggtgag gcaccatgaa
     5281 cgacgcacga tctgccgcgg tgatcccgct tcgcccgtcc gccgcggacg cgcacgcgga
     5341 tctcgtcgcg ttcctgttta ccgacatcga gggatcgagc ctcaagtggc tcaaccatcg
     5401 cgcggccatg caggaagcgc tgcgcgtcca cgacgagatc ctgcgcaagg cggtggtcgc
     5461 gcatggcggt gaggtgttca agacgggcgg cgacgcgttc agtgcggcgt ttcgccggcc
     5521 atccgacgcg gtcggcgcgg cgatcgacgc gcagcaggcg ctcgcgcgcg ccgattttgg
     5581 cagcgtggac gggttggcgg tccgcatggc cgttcacgtc ggaacggccg agcggcgtga
     5641 aggcgactac ttcggcccgg cgctcaaccg cgtcgcgcgg cttctgtcgc tcggccatgg
     5701 cggccaggtg ctcgtcacgt cgtccgcatc ggagttgatc gccgccgagc gcgagaaccg
     5761 ccacacgctg cgccatctcg gcgctcatcc gctcgacgac ccgctgcaac cggtgggcgt
     5821 gcaccaggtg gacgtgccgg gccttccgca cgaattcccg ccgctgcgca ccaccgagaa
     5881 tcgtccgacc aacctgcccc ggcagccgac gccgctgatc ggccgcgacg ccgagctcga
     5941 gcgcgtcgct gcgctcctgt cgcagaactc gctcgtcacg gtaaccggga ccgggggcgt
     6001 gggcaagaca cggatcacgc tggaagccgg tacgcgcctt ctcgaacggt ttccggacgg
     6061 tgtgtggctc gtcgagctcg ccgcgatctt cgatgcgaca ctcgttgcga gcgccgcgac
     6121 gaacgcgctc gccgtcgaca cgtcgtccgc aaagacgccg caggaggcgc tgctgtcgcg
     6181 cctcaagcac cagcatctcc tgctcctcct cgacaactgc gagcacgtga tcgaggcggt
     6241 agctgcgctc gtcgagtcgc tgctcgcggt cgcgccgaac gtgtgcgtgc tcgtcagttc
     6301 gcaggagccg ctcggcatcg ccggcgagca ggtgttccgc ctgccgtcgc tcgccgtgcc
     6361 gaaggatgcg caggttcgct cggaggacgc gatgcgcgcc ggtgcggtgc agctcttcgt
     6421 cgagcgcgcg aaaggagccg atccgcgttt tgcgctcgac gatcggaacg cggcgaccgt
     6481 ggcggcgatc tgccggcgtc tcgacggcat cccgctcgcc atcgagatgg ccgcggcgcg
     6541 cgcgccgatg ctcggcgtcg acaagctcgc acagaagctc gacgagcgct tccgcgtgtt
     6601 gaccggcggc cgacgcaccg cgctgccgcg ccagcagacg ctgcgcgcga cgctcgactg
     6661 gagctacggc cttttgagcg agcgcgagcg cacggtgctg cggcggcttt cgatcttcgc
     6721 cgggggcttc acgctcgacg gcgcagggcg cgtcgcgtcg gacgatgcga tcgacgagtt
     6781 cgaggtgatc gatcttctgt cgcatctcgt cgcgcgctcg ctcgtcgtcg ccgacaccga
     6841 cgagggcggt ggcacgcgct accggttgct cgaaacgacg cgcgcgtatg cgctggagaa
     6901 gctcgcggac gcccgggaat cgccggcgat cgagcgtttg cacgcgacgt tctttcgcga
     6961 tctcttcgag cgcgcgtacg acgactggca aacgacaccg gatgtcgaat ggcgcaacgc
     7021 ctatcttccc gagcgcgaca acctacgtaa tgcgctcgac tgggcgtttg gaccgagcgg
     7081 cgataccgcg atcggcatcg agatcgccgg ggtgacctcg gagatgtggt ggcagctttc
     7141 gctgacggcg gaggggcggc agcggatgga agcgatggcg ccgcacgtcg acgaaacgct
     7201 gccgccgcac gtcgccggcc gcttctggct cgcgttcggc cgcttctggg cgaacattgc
     7261 gccgctgcgc ggcgccgagg cgcgcgcacg agcggcggcg ctctttcgcg acgcgaacct
     7321 tccggccaag cgcgccgaag cgctcgcgtt gtacgcgcag ggactgcccg aaacgtccga
     7381 attagacgcg aagcggcaag cggcgctcgc cgaggcgtgg ccgctcgtcg agcaggcggc
     7441 gctgccgcgg ttgctcggcc aatatttcaa taccagcgcg cgttttcaca tcatttcgaa
     7501 tccagcgctg gcgcgcgatc atttcgagtc cgcgctgcgc cattaccgcc atgccggcgc
     7561 cgaggcgcag gtcctcaaca cgctgaacaa tcttgccgat ttcacatgga gcaccggcga
     7621 tctttccgcg gcggtggcga gcatgcgcga ggcggtcgtg ctggttcgcg gctcgacggt
     7681 atcggaccgg acattgcttg gccaggtgct cggcaatctc gcgggcgcgc tggtcgagca
     7741 gggcgagatc gacgaagcgc tggtcacgtt gcgcgaggcg atcccgatca tccgtgaagg
     7801 gggctggttc tttcgtttcg gcgatcatct cgcgctgcgc ctcgcgaagg ccggccacgc
     7861 cgatgcggcc gcacgtttgc tgggctatgc ggatgccgag cacgcgcggt tcgaggccaa
     7921 ccggcaggtc aacgaggcgc gcgcgcatga gagcgtgctg gcgatcctga acgcggcgat
     7981 cgcccccgcc gagctcgcgc agcggatggc cgagggcgcg aagctctccg aggacgaggc
     8041 ggcgcggctc gcgctcgagc cgtgacgcga tggcgtatcg cgacctgcgc gatttcatcg
     8101 cgcaactgga agccgccgag gatctgaagc gcgtcgctgc gccagtcgat ccgaacctcg
     8161 agatgacggc gctctgcgat cgcgcgttgc gcgcgcaggg accggcgttg ctgttcgagc
     8221 atccgaaggg cgatcggcgc ggcccgagcg gcaacgcgat tcccgtgctc ggcaacctgt
     8281 tcggcacgcc cgagcgcgtg gcgaaggcga tgggcgcgga tgccgcgaac tggcaaaagt
     8341 cactgcgcga gaccggggag ctgctctcgt ttctgcgcga gccggcaccg ccgaagagcc
     8401 tcgtcgatgc atggcgcaat acgcggcccg tgctgatgaa ggtgctcgac atgacgccga
     8461 agctgcgtca tggcggaccc tgccaggagg tcctgtggga gggcgacgag gtcgatcttg
     8521 cgcgactgcc gatccagacc tgctggcccg gcgatgccgg ccccctgatc acatggggac
     8581 tcaccgtgac gcgcgggccg cacaagaagc ggcagaacct cggcatttac cgccagcagg
     8641 tcatcgcgcg caacaaggtg atcatgcgct ggctcgctca ccgcggcggt gcgctcgact
     8701 ttcgcgatca cgcgatcgcg gcacccggaa cggcgttccc gatcgcggtg gcactgggtg
     8761 ccgatcccgc gacgatcctc ggcgccgtga cgccggtacc cgatacgttg tcggaatacc
     8821 agttcgccgg attgctgcgc ggcggccgca ccgagatcgt caagtgcctg acgcacgatc
     8881 tgcaggtgcc cgcgagcgcc gagatcgtgc tggagggctt catccatccc gatgcgaacg
     8941 atccgtccgg ttacgaaacc gcactcgaag gcccgttcgg cgaccacacc ggctattaca
     9001 acgagaccga gcgcttcccg gtgttcacga tcgaccggat gacgctgcgc cgcgatccga
     9061 tctatcactc gacgtatacc ggcaagccgc ccgacgagcc ggcgatgctc ggtgtcgcgt
     9121 tgaacgaagt gttcgtgccg ctgctgcaga agcagtatcc ggaaatcagc gacttctacc
     9181 tgccgccgga aggctgcagc taccggctcg cggtcgtcgc gatgaaaaag cagtacccgg
     9241 ggcacgcgaa gcgcgtgatg ttcggcatct ggagcttcct gcgccagttc atgtacacga
     9301 agatgattgt cgtcaccgac gacgacgtcg acatccgcga ctggaaggac gtgatctggg
     9361 cgctcaccac gcgggtcgac ccggcgcgcg atacgctgat cgtgccgaac accccgatcg
     9421 actacctcga cttcgcgtcg ccggtcgcgg ggctgggcag caagatcggc atcgacgcga
     9481 cgaacaagtg gcccgccgag accgcgcgcc agtggggacg tccgatcgcg atggacgccg
     9541 acgtcaccgc gcgcatcgag gcgatctggc ggacgatttc gacttcgggg tcagagctgt
     9601 aattattcgc acgaacggat aattacagct ct
//
LOCUS       DASSVX010000331         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_398319, whole genome shotgun sequence.
ACCESSION   DASSVX010000331 DASSVX010000000
VERSION     DASSVX010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398319"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VFO53_14230"
     CDS             complement(<1..1302)
                     /locus_tag="VFO53_14230"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8877482.1"
                     /translation="MNDARRLLTVMFTDIESSSRLWEQHGARMAAAVARHDALVRAAV
                     HEHRGSIVKMLGDGAHAVFADPCDALLASLQLLRAVGDPSSTADVPLRIRCGVHAGIV
                     ECRDNDYFGTVVNRAARLASAAHGGQLLVSRAVVELAGGRLPPDVSLRDLGNVRLRDL
                     ASPEHVYQLLHADLRREFPPLRSLEATPNNLPQQVTSFIGHAVSRVEVRRLFATARLL
                     TLVGMGGLGKSRLSLQVATEVLDDFADGAWLVELAAIADARRVPEAVASVLGVVEDPG
                     RSVREALLRYVKDRQLLVILDNCEHLIDACAELAKALLTAGPRVTIMASSREPLRITG
                     EVIYPMPALSLPKLGASVDVALVQQCDAVHLFIERAVAVQRHFAITADNAPAVADICR
                     RLDGIPLAIELAAARSNVLAVETIAARLHDRLGLLTRGSRAV"
     gene            1478..2011
                     /locus_tag="VFO53_14235"
     CDS             1478..2011
                     /locus_tag="VFO53_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET8877483.1"
                     /translation="MSLWKDNTAPRQAPSPAPELKEPTRFDAASKPEFAANPASPPVT
                     RSEPAPARKESLIAADITIEGKIEGGGSVRIAGKFKGDVNVQGDLTIESGAKLTGSVR
                     ADKVTIAGELEGNVEEATLVNLLDSGIVVGDLRAGSLTVAAGARLRGQAEFGWDDSGA
                     QKGKQRIGHRTESGTVA"
     gene            2008..>2363
                     /locus_tag="VFO53_14240"
     CDS             2008..>2363
                     /locus_tag="VFO53_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877484.1"
                     /translation="MSSARPGSAGATRACPHCRETILESASVCPACRKFLRFDGNSDE
                     PAVAALTPLSIEGSIRHPADGEPWEYTVVVAIRNDRGEETDRKLIGVGALNPNEQRTF
                     TLSVEVVSAKGKRAGRG"
BASE COUNT          440 a          792 c          784 g          347 t
ORIGIN      
        1 cacggcgcgg ctgcctcgtg tcaacagacc caatcggtca tgaaggcgtg cagcaatcgt
       61 ctcgacggca agcacgttgc tgcgcgcggc ggcgagttcg atcgcgagcg gaatgccgtc
      121 gaggcggcgg cagatgtcgg caaccgccgg tgcgttgtca gcggtgatgg cgaagtggcg
      181 ttgcaccgcg acagcccgct cgatgaagag atgcaccgcg tcgcattgct ggacgagtgc
      241 gacgtctacg gaagcaccca gctttggcag cgaaagcgcc ggcattgggt aaatcacttc
      301 gccggtgatg cgcagcggct cgcggctcga cgccatgatc gtgactcgcg gacccgcggt
      361 gagcagcgcc ttcgcgagct cggcgcacgc atcgatcaga tgctcgcaat tgtcgaggat
      421 caccagcaat tgccgatcct tgacgtaacg caggagcgcc tcgcggaccg atcgtcccgg
      481 atcttccacg acgccgagca cggacgcgac cgcttcgggt acgcgccgtg cgtcggcgat
      541 ggcggcgagc tcgaccagcc aggcgccatc cgcgaaatcg tccagcacct ccgtcgccac
      601 ctgcagcgac agccgcgatt tgccgaggcc gcccatgccc accagcgtca gaagacgtgc
      661 ggtggcgaac agccgccgca cttcgacgcg gctcacagcg tgcccgatga aagacgtcac
      721 ctgctgcggc aggttgttcg gcgttgcctc gagcgatcga agtggcggaa attcgcgccg
      781 aaggtcggcg tgcagcaact gatagacgtg ctcgggactc gcaagatcgc gcaaccgtac
      841 gttgccgaga tcacgcagcg aaacgtccgg cggcaggcgt ccaccggcaa gctcgaccac
      901 tgcgcgcgag accagcagtt gcccgccgtg cgcggcactc gcaagtcgtg cggcgcgatt
      961 gacgacagtg ccgaagtagt cgttgtcgcg gcactcgacg atgccggcat gcacgccgca
     1021 gcggatccgc aacggaacgt ccgccgtcga cgatgggtcg ccgacggcgc ggagcaactg
     1081 cagcgatgcg agcagcgcat cgcagggatc ggcaaaaacg gcgtgcgcgc catcgccgag
     1141 cattttcacg atgctgccgc gatgctcgtg gaccgccgcg cgcaccagtg catcgtgtcg
     1201 cgcaaccgcc gctgccatgc gggcgccgtg ctgttcccac aaccggctgc tgctctcgat
     1261 gtcggtgaac atcaccgtca gcaaccggcg ggcatcattc atcttcgagc gtcacgaacg
     1321 cggcgacgaa cccgatgtcc ggaccgcctt cgacctccga gtcgttgcgg attcgcctcc
     1381 gacgatacac ggcaaaccgg gtgcagcgcc cggattcatt gcctccgacc cccttttagc
     1441 gctagcctga aaaaccctgt gttcgatagg actcgccatg tccctctgga aagacaatac
     1501 cgcgccgcga caggcaccct cgcctgcccc cgagctcaag gagcccactc gcttcgacgc
     1561 cgcgtcgaag ccggaattcg ccgccaatcc ggcgtcgccc ccggtcacgc gttcggagcc
     1621 tgcaccggca cgcaaggaat cgctgatcgc cgccgatatc acgatcgaag gcaagatcga
     1681 aggcggcggc agtgtgcgaa tcgccggcaa gttcaagggt gacgtcaacg tccagggcga
     1741 cctgacgatc gagtcgggcg cgaagctgac cggtagcgtg cgcgccgaca aggtcacgat
     1801 cgcgggcgag ctcgaaggca atgtcgagga agcgacgctc gtcaacctcc tcgattcggg
     1861 catcgtcgtc ggcgacctgc gtgcgggctc gctcaccgtc gcggccgggg cacgcctgcg
     1921 cggccaggcc gagttcggct gggacgacag cggcgcgcag aaaggcaagc agcggatcgg
     1981 tcatcggacg gagagcggca ccgtcgcatg agcagcgcgc gcccgggcag cgccggggcg
     2041 acgcgtgcat gtccgcactg ccgggaaacg attctcgaaa gcgcgagcgt ttgccccgct
     2101 tgtcgcaagt tcctgcgttt cgacggcaac agcgatgagc cggccgttgc cgcgctgacg
     2161 ccgctcagca tcgaaggcag catccgccac ccagccgacg gcgagccgtg ggaatacacg
     2221 gtcgtcgtcg cgatccgcaa cgatcgcggc gaagagacgg atcgcaagct gatcggtgtc
     2281 ggtgcgctga atcccaacga gcagcgcacg ttcacgctgt cggtcgaagt cgtgtcggcg
     2341 aaaggaaagc gcgccggccg cgg
//
LOCUS       DASSVX010000332         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_631651, whole genome shotgun sequence.
ACCESSION   DASSVX010000332 DASSVX010000000
VERSION     DASSVX010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631651"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VFO53_14245"
     CDS             complement(<1..956)
                     /locus_tag="VFO53_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET8877485.1"
                     /translation="MASPDLLPAYAELHCLSNFSFLHGASHPEELVERAQAEGYSGLA
                     LTDECSLAGAVRAHLAAKDAGLPLVLGSEFTLDDGVKLVLLATDRASYGDLSQLITRG
                     RRQAAKGTYRLTRGDVAEQASSCLALWLPALDLRASDIGDALRHDAAWMREVFADRGC
                     IGVELLARAGDAVRLAQCTALAHMSALPLVAAGDAHMHVRARRALQDTLTAIRLAMPL
                     AQCGHALYPNGERHLRSRARLASIYPPALLAATVRIAERCSFSLDELRYEYPEEIVPP
                     GETAASHLRKLVERGLASRYAESETPNRGPKVGSETTFHRAAG"
     gene            1075..>2190
                     /locus_tag="VFO53_14250"
     CDS             1075..>2190
                     /locus_tag="VFO53_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015153607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HET8877486.1"
                     /translation="MPPIAFDRFYRYDELRDLLHALAREHPDLVAIESIGKSHEGRDI
                     FVLTVTNTATGPASEKPAFWVDGNIHSTEVAASAAALYFLNTLVTQYGKDADVTRALD
                     TRAFYVCPRINPDGAEWALADRPKWVRSSTRPYPHDEEEPEGLGVEDVDGDGRILQMR
                     IADPNGLWKAHPDEPQLMVRREPTETGGTYYRIIPEGRYDNYDGHTLKIKRPRERLDL
                     NRNFPASWRQEFEQAGAGPYPTSEPEIRAVVDFVVRHPNITAGTTFHTWSGVLLRPFE
                     HLSDDEMDAEDLWFYQKTGRKGSELTGYPAISVYHEFRYHPKSVIGGTFDWLYEHLGA
                     FSWVVEIWSPMREAGITNYKFIDWFRDHPIADDLKLYR"
BASE COUNT          400 a          743 c          700 g          347 t
ORIGIN      
        1 ccggcagcac gatgaaaagt cgtctctgac cccacttttg gaccccgatt cggcgtctcc
       61 gactccgcat agcggctcgc aaggccgcgc tcgacgagct tgcgcagatg cgacgcggcc
      121 gtttcgccgg gcggcacgat ctcctccgga tactcatagc gcaattcgtc gagcgagaag
      181 ctgcagcgct cggcgatcct gaccgttgcg gcgagcaacg ccggcggata aatgctcgca
      241 agccgcgcgc gcgagcgcag atgccgctcg ccgttcggat aaagcgcatg gccgcactgc
      301 gcaagcggca tggcgagacg aatggcggtc agcgtgtcct gcagcgcgcg gcgcgcgcgc
      361 acgtgcatgt gtgcgtcacc cgcggcgacg agcggtaatg cactcatgtg cgccagcgcc
      421 gtgcattgcg cgagccgtac ggcatcgccg gcccgcgcga gcagctcgac gccgatgcag
      481 ccacgatcgg cgaatacttc ccgcatccac gccgcatcgt gtcgcaacgc gtcgccgatg
      541 tccgacgcgc gcaaatcgag cgcgggcagc cacaatgcaa gacagctcga cgcctgttcg
      601 gcgacatcgc cgcgcgtcag ccggtatgtg cccttcgccg cctgccgccg cccgcgcgtg
      661 atcaactgcg acaggtcgcc gtagcttgcg cgatcggtcg cgagcagcac gagcttcacg
      721 ccgtcgtcga gcgtgaactc gctgccgagc acgagcggca atcccgcgtc cttcgcagcg
      781 agatgcgcgc gcacggcgcc ggcgagcgag cattcgtcgg tcaacgcgag gccgctatag
      841 ccctcggctt gggcgcgctc gacgagctcc tcgggatgcg acgcgccgtg caggaacgag
      901 aaattcgaca ggcagtgcag ctcggcatag gccggcagga ggtcgggcga tgccataccg
      961 catgaatata cggtatcggg cgccgtttga ccagttttcg ccgcacgttg acgtaagatg
     1021 caacggcccc gcgcccgact ccccgtcttt tgctcgccgc tccagattgc caccatgccg
     1081 cccatcgcat tcgaccgctt ctaccgttat gacgaactcc gtgacctgct gcacgcgctt
     1141 gcgcgcgagc atccggacct cgttgcgatc gagtcgatcg gcaaaagcca cgaaggccgc
     1201 gatatcttcg tgctcacggt gacgaacacc gccaccgggc cggcaagcga gaaaccggcg
     1261 ttctgggtcg acggcaacat tcattcgacc gaagtcgcgg cgtcggccgc ggcgctttac
     1321 ttcctgaaca cgctggttac gcaatacggg aaggacgccg acgtgacccg cgcgctcgac
     1381 acgcgcgcgt tctacgtctg cccccgcatc aatcccgacg gcgccgaatg ggcgctcgcc
     1441 gacaggccga aatgggtgcg ctcgagcacg cggccttacc cgcatgacga ggaggagccg
     1501 gagggactcg gcgtcgagga cgtcgacggc gacggacgca tcctgcagat gcgcatcgcc
     1561 gacccgaatg gactgtggaa agcgcatccc gacgagccgc aactgatggt gcgccgggag
     1621 ccgaccgaaa ccggcggcac gtactaccgg atcattccgg agggccgcta cgacaactac
     1681 gacggccaca cgctgaagat caagcgcccg cgcgagcggc tcgatctcaa tcgcaatttt
     1741 ccggcgagct ggcgccagga attcgagcag gcgggcgcgg ggccgtaccc gacgtcggag
     1801 cccgagatcc gcgcggtcgt cgatttcgtc gtgcgccatc cgaacattac ggcgggcacg
     1861 acgttccata catggagcgg cgtgcttttg cggccgttcg agcacctgtc cgacgacgag
     1921 atggacgccg aggacctgtg gttctatcaa aagaccggac gcaagggcag cgagctgacc
     1981 ggttatccgg cgatctccgt gtatcacgag ttccgctatc acccgaagtc ggtgatcggc
     2041 ggcacgttcg actggctcta cgagcacctc ggcgcgttct cgtgggtcgt cgagatctgg
     2101 tcgccgatgc gcgaggcggg catcaccaac tacaaattca tcgactggtt ccgtgatcat
     2161 ccgatcgccg acgacctcaa gctctatcgc
//
LOCUS       DASSVX010000333        12438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_248177, whole genome shotgun sequence.
ACCESSION   DASSVX010000333 DASSVX010000000
VERSION     DASSVX010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 12438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12438
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248177"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(378..1685)
                     /locus_tag="VFO53_14255"
     CDS             complement(378..1685)
                     /locus_tag="VFO53_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HET8877487.1"
                     /translation="MDEFSLGTLALALIILLVSSGFFSIAETAMMAANRYRLKHQAAR
                     GARGAKLALSLLAQTDRLLGVILLGNNLINAAAATLASIITVRLFGQGEWALALGTVA
                     VTFLILVFSEITPKVVGAAYADKLAPLVSYALVPLLRTVRPVVWFVNLFVQGLLRLFR
                     FRPAAQPSTILSQEELRSLVLEGAQYFRGKHKTMLANLMDLEAITVDDVMTPRSQIHA
                     LDLNEKPALLRQQLVTSYHTRLPVCEDSLDNIVGVLAVKSILHAMQAGGEIDAERIRA
                     VLRPPYFVPLGTPLLTQLSQFQADRQRLGLVVDEYGEMQGLVTIEDIIEEIIGEFTTQ
                     APGAPESFRREPDGSVVVDGMAPLRTLNRRLGTRFPLDGPKTLNGLIVEHLGEIPDAG
                     THFDVDGQAVEIMQTQDRAVRVVRLLPPIGRGTAPLQTQAERA"
     gene            complement(1757..2557)
                     /gene="ccsA"
                     /locus_tag="VFO53_14260"
     CDS             complement(1757..2557)
                     /gene="ccsA"
                     /locus_tag="VFO53_14260"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET8877488.1"
                     /translation="MIILLHVLVAAIYAATAWVRWPRGERAAPRRNAGQALLAVALML
                     HAVAIGQAIVRRDGLDLSFVNALSLVAGLAVLAAWLTGVLRTLPGVAAVVLPVAAVCA
                     LLPSLALSHHRVAFTGEPWAAVHIGVALVAYALFVVVALQALVMTGLEKRLHRGEPDM
                     AAATAPPLLTLERYMFRLLTVGFVLLTLTLASGMLFSEEIFGKAVTLTHKNVFSVAGW
                     IAFAVLLFGRWRYGWRGRTALKWILAGTLLLVLGYLGSKFVREVVLGR"
     gene            2616..3956
                     /gene="ffh"
                     /locus_tag="VFO53_14265"
     CDS             2616..3956
                     /gene="ffh"
                     /locus_tag="VFO53_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET8877489.1"
                     /translation="MLETLTERLSRVVKTLRGEARLTEANIQDALREVRLALLEADVA
                     LPVVRTFIADLREKAIGADVAGSLTPGQALVGVVARELTALMGGTAQPLDLATRPPAV
                     ILLAGLQGAGKTTTAAKLARLVAGQKKKVLLVSTDVYRPAAIEQLKLLAAQVGVDFFP
                     STKDEQPVTIAGAALDWARRHYHDVLIVDTAGRLSIDEAMMREIADLHRAAQPVETLF
                     IVDAMQGQDAVNTARAFHDTLPLTGVILTKLDGDARGGAALSVRQVTGAPIKFAGVSE
                     KLDGLEVFHPERMASRILGMGDVLSLIEDAHRNVDVEQAKKLARKVKTGKGFDLDDFK
                     AQIAQMRKMGGLAALVDKLPAEIARAAQSATLDERRIARIEGIINSMTHQERVKPELL
                     KASRKRRVAAGAGVTVQEVNQLLAQFEQTQKMMKMVSKGPQKMMRGLRNLMPGR"
     gene            4092..4391
                     /gene="rpsP"
                     /locus_tag="VFO53_14270"
     CDS             4092..4391
                     /gene="rpsP"
                     /locus_tag="VFO53_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET8877490.1"
                     /translation="MVVIRLSRGGAKKRPFYNVVVTDSRNRRDGRFIERVGFYNPVAA
                     AHEEGLRIVHDRVAYWQQHGARLSDTVQTLVARAAKPAAAAAPVEAAAPASAQPA"
     gene            4471..4995
                     /gene="rimM"
                     /locus_tag="VFO53_14275"
     CDS             4471..4995
                     /gene="rimM"
                     /locus_tag="VFO53_14275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET8877491.1"
                     /translation="MTPAEPARLVVMGRVGAPHGVHGAVKVRPESADPASLCTYGIWW
                     LRRRDEPSWRAHSVRSVRGQGEWLVAELAGIDSREAAGALRGGEVAIPRESLPALAAD
                     EYYQADLAGMAVVNRDGVSLGALRGFLESGAHPIARVVDDEGRERLIPWVADYIDAVD
                     ASARRIEVDWPADA"
     gene            5052..5813
                     /gene="trmD"
                     /locus_tag="VFO53_14280"
     CDS             5052..5813
                     /gene="trmD"
                     /locus_tag="VFO53_14280"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET8877492.1"
                     /translation="MRIDVVTLFPQMLTQASAYGVTGRARERGLWQLGVWNPRDFATD
                     AYRTIDDRPYGGGPGMVMMAAPLARALGAAREAQRAAGVAASRTLYLSPAGTPLSHRA
                     VMDLAGAPGVALTLLAGRYEGVDQRLIDREVDAEVSIGDFVVSGGELPALLLIDAIVR
                     QLPGALNDAESAQQDSFADGLLDCPHYTRPEHHDGETVPPVLLSGDHAAIRRWRLKQS
                     LGRTWQRRPDLLGRRALSHEEQALLSQYQVELTDN"
     gene            5885..6271
                     /gene="rplS"
                     /locus_tag="VFO53_14285"
     CDS             5885..6271
                     /gene="rplS"
                     /locus_tag="VFO53_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET8877493.1"
                     /translation="MNIIETLEREEIARLNKTIPEFAPGDTVIVNVNVVEGERRRVQA
                     YEGVVIAKRNRGLNSAFTVRKISSGEGVERTFQTYSPLIASIEVKRRGDVRRAKLYYL
                     RGRSGKSARIREKLAVRETAKPEQTS"
     gene            6503..8374
                     /locus_tag="VFO53_14290"
     CDS             6503..8374
                     /locus_tag="VFO53_14290"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET8877494.1"
                     /translation="MKATLYDALGILPTSSDEEVRAALRGLIRKYYAKTRDGQGNVEE
                     ALRFINHASRILSDGERRERYDEELSRSTGDEDDPARLAAKRAVFGDDADSEAGPPTG
                     VGDDAIADPLDSELPGDKADVPATHHPGLTERVASFGRSRLLTYGLCALFGGFIATAI
                     VFVTPPNFVVVARSVLGSLVVVFLLLAAVYAIVHGLGLVRRRRMGTRSALVPQTDLAI
                     LNWRRERSVFLGTNQPQEDASWIFQLRMAELERAKSGRTSEPRPWHRLAARMFDYAIW
                     GLLLALLLSQLRAAGLVPELTAHWLAHPLIAPILITLSFVPIEALLITAVGTTPGKWL
                     FGIYLQFSISDAYARRDTRMQLLRALLRACRVWFQGLGCGFPLLAPILVAVAHERLVL
                     HQETDWDFAEDCLVTQGPAGVVNIVTGVCGLASMMWLYGVAWQQPMADAVAWTKASVI
                     AAVPSRDGLESRLGQASGRFEHFIPALSPRSVSDALRLPWTPAPSGTGTSTTAPAALR
                     EVAPETGPSDAEFTSMMAARRARIDLLKSEGPRLLRAGNARRALDVCRAWVELDLANS
                     EAWRCLGQAQLASGQHQEALNSFRKARQHNPSDRSLDAAIQQAERSIVADFQRRYGR"
     gene            complement(8433..8660)
                     /locus_tag="VFO53_14295"
     CDS             complement(8433..8660)
                     /locus_tag="VFO53_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET8877495.1"
                     /translation="MTIDREIDARGLNCPLPILRTKKALNDMASGQMIRITATDPASV
                     RDFQAFARQTGNELLEHGEDDGAFWFVLKRR"
     gene            8712..10121
                     /locus_tag="VFO53_14300"
     CDS             8712..10121
                     /locus_tag="VFO53_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET8877496.1"
                     /translation="MRYVLPVLLVVVVVAVGAASVAIDRVDVPPRRLGPWVERRAEGH
                     HDILQRGGREISAVLLALDRGREAFHAPLPVPAVGDGEPPTAKRRVIVASTEEALHAI
                     DDARPGDDITFVPGTYRFDGAYIDAHEAGAPGAPITVSADEPHSVVLEFNMIEGFLVS
                     APYWSFANLTIRGVCSDHSNCEHAFHVVGAAGHFVARANELVDFNAHFKINGMRGAYP
                     DWGLIEGNVIRNTGPRETANPVTPVDLVAASHWRIRGNRISDFVKRRGDGTSYGAFVK
                     GGGSDNRLEDNVVVCEDRLRGFPGQRVGLSLGGGGSSRDACRDGRCITEQDEGVIATN
                     LIASCSDGGIYLNRAATSVITHNTLIDTAGITARFAESAADVEGNLIDGPIRAVDGAL
                     LHRTDNRITGVAPLYLGLHPVRDLFVDAPTLDLRWRGDPPRRQRAASQPPDLCGTPRP
                     ARPVYGAFEDIARCAASRN"
     gene            complement(10139..10639)
                     /locus_tag="VFO53_14305"
     CDS             complement(10139..10639)
                     /locus_tag="VFO53_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HET8877497.1"
                     /translation="MVNPPVSLPEPRYPNKIADAAHALARIGALARPLVFTNGVFDIL
                     HRGHVTYLEQAKGLGASLVVAVNSDASAKRLGKGADRPLNGQDDRMAVLAALASVDLV
                     VAFDADTPHDLIVECAPDVLVKGGDYSAATTAGAAEVIARGGRFVAIPFRYERSTSAL
                     VKRIRG"
     gene            complement(10680..11261)
                     /locus_tag="VFO53_14310"
     CDS             complement(10680..11261)
                     /locus_tag="VFO53_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET8877498.1"
                     /translation="MRKRLIFAAFAFLACGSIVLSGCVPLVIAGAAGGAALVAADRRS
                     VGAQTDDEAIELKMANNIGAGYGDRVHVNVTSYNGIVLLTGEVPSPELKRSIEEIAKS
                     TAKVRSVTDELSVGPVSSIGSRTNDSYITSKVKARFVEANKFAPNHVKVVTERQVVYL
                     MGLVRRAEADAAAQIAATTSGVVRVVKLFEYID"
     gene            complement(11258..11854)
                     /locus_tag="VFO53_14315"
     CDS             complement(11258..11854)
                     /locus_tag="VFO53_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HET8877499.1"
                     /translation="MIDHLERIRGHFDDSAALKQAAAQTMAAPIARAGAVLTDCLFAD
                     GKILACGNGGSAADAQHFAAELVGRFERERPELPAIALTTDSSLLTAIANDYSFEQIF
                     AKQVRALGAKGDVLLAISTSGNSANVIAAVVAAHEREMRVVALTGKGGGRIGELLAPE
                     DVHLCVPHARTMRIQEVHLLTIHCLCDVVDATLLGDDA"
     gene            complement(11959..12309)
                     /locus_tag="VFO53_14320"
     CDS             complement(11959..12309)
                     /locus_tag="VFO53_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET8877500.1"
                     /translation="MITAGGRAEALAAAFLLQQGLAIVARNFRTRFGEIDLIARDGRV
                     LVFVEVRLRRSTRYGGAVESITPRKRARLVAAANGYLAMLGREPPCRFDAILMRSLDA
                     ATIEWRRDVIAVDG"
BASE COUNT         2012 a         4180 c         4281 g         1965 t
ORIGIN      
        1 cctgctcgac gaacgacacg ccggacgatt gcgactgcat ctgcagcgcc tcggcctcgt
       61 gctcggacat gaccccgtgc tgcgcaagcg cgcgcgcaag gccgctgatg tttcgggtgg
      121 cggcgtgggt tgccatgtca ggcgagaagg atgtgtcgca tttttgccac taattgcgca
      181 ggagtccgcc ggcttgcgtg gagcaacctg atcgaaacgt cagtcaaagc aaacgtcatg
      241 ccgtttatcg gcacttggcg gccgattccg gtgcctttgg tgccgggcgc gcactcgtcg
      301 cgtctcgtgc gttgccgcga gcgaagtgcc cggcgccggc cacgcgtatt ccattcgcat
      361 aaaaaatcgc gtcaggatca tgcccgctcg gcctgggttt gtaaaggcgc ggtgccgcgt
      421 ccgatcggcg gcaacaggcg caccacccgc accgcgcggt cctgggtttg catgatctcg
      481 accgcctggc catcgacgtc gaagtgggtc ccggcgtcgg gtatttcgcc cagatgctcg
      541 acgatcaggc cgttcagcgt cttcgggccg tcgagcggga agcgtgtgcc gaggcggcgg
      601 ttgagcgtgc gcagcggcgc cataccgtcg acgacgacgc tgccgtcggg ctcgcgccgg
      661 aagctctcgg gggcgcccgg cgcctgcgtc gtgaactcgc cgatgatctc ctcgatgatg
      721 tcctcgatgg tgacgagccc ctgcatctcg ccgtattcgt cgacgacgag gccgagccgc
      781 tggcggtcgg cctggaactg cgacagttgc gtgagcagcg gcgtgccgag cggcacgaaa
      841 tagggcgggc gcagcaccgc gcgaatgcgc tcggcgtcga tctcgccgcc ggcctgcatc
      901 gcgtgcagga tgctcttgac cgcgagcacg ccgacgatgt tgtcgagcga gtcttcgcac
      961 acggggagcc gcgtgtggta cgacgtgacg agctgctggc gcagcagcgc cggcttttcg
     1021 ttgaggtcga gcgcgtggat ctggctgcgc ggcgtcatca cgtcgtcgac ggtgatcgcc
     1081 tcgaggtcca tcaggttggc aagcatcgtc ttgtgcttgc cgcgaaagta ctgtgcgccc
     1141 tcgagcacca gcgagcgcag ctcctcctgc gaaagtatcg tgctcggctg cgcggccggc
     1201 cggaagcgga acagccgcag cagcccctgc acgaagaggt tcacgaacca gacgaccggg
     1261 cgcaccgtgc gcaggagcgg cacgagcgcg tagctgacga gcggcgcgag cttgtcggcg
     1321 taggctgcgc cgaccacctt cggcgtgatc tcggagaaga cgaggatcag gaacgtgacg
     1381 gccaccgtgc cgagcgcgag cgcccattcg ccctggccga acaggcgcac ggtgatgatg
     1441 cttgcgagcg tcgccgcggc ggcgttgatg aggttgttgc cgagcaggat cacgccgagc
     1501 agccggtcgg tctgtgcgag cagggacagc gcgagcttgg cgccgcgggc gccgcgcgcc
     1561 gcctggtgct tcagccggta tcggttcgcc gccatcatcg ccgtttcggc gatcgagaag
     1621 aagcccgagc tcaccagcag gatgatcagc gcaagcgcaa gcgtgccgag ggagaattcg
     1681 tccaagggat aagtggggtc agagacgact tattgcctgc gtcgcgctgg ttccaataag
     1741 tcgtctctga ccctgcttat cgcccgagga cgacctcgcg cacgaacttg ctgccgaggt
     1801 agccgagcac gagcagaagc gttccggcga gaatccattt tagcgccgtg cgcccgcgcc
     1861 agccatagcg ccaacggccg aacagcagca cggcgaacgc gatccatccc gccacggaga
     1921 acacgttctt gtgcgtgagc gtgacggcct tgccgaagat ttcttcggag aacagcatgc
     1981 cgctggcgag cgtcagtgtc agcagcacga agccgacggt gagaagccgg aacatgtagc
     2041 gctcgagcgt gagcagcggt ggcgcggtcg cggccgccat atcgggctcg ccgcggtgca
     2101 gccgcttttc gagtcccgtc atcacgagcg cctgcagcgc gacgacgacg aagagcgcat
     2161 acgcgacgag cgcgacgccg atgtgcacgg cggcccacgg ttcgccggta aacgcgacgc
     2221 gatgatgcga tagcgcgagc gacggcagca gcgcgcacac cgcagcgacc ggcagcacga
     2281 cggccgcgac gcccggcagc gtgcgcagca cgccggtcag ccacgcggcg agcacggcga
     2341 gaccggccac cagcgacagc gcgttgacga acgacaggtc gagcccgtcg cgacgcacga
     2401 tcgcctgccc gatcgcgacc gcgtgcagca tcagcgcaac ggcgagcagc gcctgcccgg
     2461 cgttgcgccg cggcgctgcg cgttcaccgc gcggccagcg tacccacgcg gtggccgcgt
     2521 agatcgccgc gacgagcacg tgcagtagaa tgatcaccga tccagtttac ctgagcgcgc
     2581 cggcgttcct gcacgcggcg cgaggtccct tttccatgct cgaaacgctc accgaacgcc
     2641 tgtcgcgcgt cgtcaagacg ctgcgcggcg aagcccgcct caccgaggcg aacatccagg
     2701 acgcgctccg cgaagtgcgc ctcgcgctgc tcgaggccga cgtcgcgttg cccgtcgtgc
     2761 gcacgttcat cgccgacctc cgggagaagg cgatcggcgc cgacgtcgcc ggatcgctga
     2821 caccgggaca ggcgctcgtc ggtgtcgtcg cacgcgagct gaccgcgttg atgggcggca
     2881 cggcacagcc gctcgatctc gcgacgcgtc cgccggccgt gatcctcctc gccggcctgc
     2941 agggcgcggg caagacgacg accgcggcga agctcgcgcg ccttgtcgcc ggacagaaga
     3001 aaaaggtgct gctcgtgtcg accgacgtgt accggccggc ggcgatcgag caattgaaat
     3061 tgcttgcagc gcaggtcggc gtcgatttct tcccgtcgac gaaggacgag cagccggtga
     3121 ccatcgccgg cgccgcgctc gactgggcgc gacggcacta tcacgacgtg ctgatcgtcg
     3181 acaccgccgg ccgcctgtcg atcgacgagg cgatgatgcg cgaaatcgcc gatctgcacc
     3241 gggccgcgca gccggtggaa acactcttca tcgtcgacgc aatgcagggc caggacgcgg
     3301 tcaacaccgc gcgcgcgttc cacgacacgc tgccgctgac cggcgtcatc ctgaccaagc
     3361 tcgacggcga cgcgcgcggc ggcgcggcac tatcggtgcg ccaggtgacc ggtgcaccga
     3421 tcaagttcgc cggcgtgtcg gagaagctcg acggcctcga agtcttccac cccgagcgga
     3481 tggcgtcgcg catcctcggc atgggcgacg tgctgtcgct gatcgaggat gcgcatcgca
     3541 acgtcgacgt cgagcaggcg aagaagctcg cgcgcaaggt caagacgggc aagggcttcg
     3601 atctcgacga tttcaaggcg cagatcgcgc agatgcgcaa gatgggcggg ctcgcagcgc
     3661 tcgtcgacaa gctgcccgcc gagatcgcgc gtgcggcgca gagtgcgacg ctcgacgagc
     3721 ggcgcatcgc gcgcatcgag ggcatcatca actcgatgac gcaccaggag cgcgtgaagc
     3781 ccgaattgct gaaggcgtcg cgcaagcgtc gcgtggcggc gggcgcgggc gtcaccgtgc
     3841 aggaggtgaa ccagctcctc gcacagttcg agcagacgca gaagatgatg aagatggtgt
     3901 cgaagggtcc gcagaagatg atgcgcggcc tgcgcaacct gatgcccggc cgctgagctc
     3961 ccggagcgcg tcccgcggag cgcgtcccgc gtttgcgtcc acggtcagtt tcgccgtata
     4021 atcatcgact tttctttttg cggccacgct tggaactcga agcccaggcg cggcgcggac
     4081 tggacgaacc catggtagtg atcagattgt cccgcggcgg cgcgaaaaag cgcccgttct
     4141 acaacgtggt ggtgaccgat tcgcgcaacc ggcgcgacgg gcgtttcatc gagcgcgtcg
     4201 ggttctacaa ccccgttgcg gccgcacacg aggaaggcct gcgtatcgtg cacgatcgcg
     4261 tcgcgtactg gcagcagcac ggcgcgcggc tttcggacac cgtgcaaacg ctcgtcgcac
     4321 gtgcggcgaa gcccgccgca gccgcggcgc cggtcgaggc cgcggcaccg gcgagcgcgc
     4381 agcccgcctg agcgtcgcac ggctgcccga ggcgcggacg ccttgatcag gagcgcggcg
     4441 gcgaagcgcg cggcctcgga agaggtaaca gtgacgccgg ccgagcccgc gcgactcgtc
     4501 gtcatgggtc gcgtcggcgc accgcacggc gtgcacggcg cggtcaaggt gcggcccgaa
     4561 tcggccgatc cggcgtcgct ttgcacctac ggcatctggt ggctgcgccg gcgtgacgaa
     4621 ccatcgtggc gagcgcattc ggtgcgaagc gtgcgggggc agggggaatg gctggtggcg
     4681 gaactcgccg ggatcgattc gcgtgaggcg gccggggcgc ttcgcggcgg cgaggtcgca
     4741 attccgcgcg aatcgttgcc ggcgctggcg gcagacgagt attaccaggc cgacctggcc
     4801 ggcatggcgg tcgtcaatcg cgacggggtg tcgctcggcg cgttgcgcgg cttcctcgaa
     4861 agcggcgcgc atccgatcgc gcgtgtcgtc gacgacgagg gccgggagcg gctcattccg
     4921 tgggtcgccg attacatcga cgccgtggat gcgagcgcgc gccggatcga ggtcgactgg
     4981 ccggccgatg cctgacgtgc cgtgcgggcg gcgagcgcgc agtggaggtg gggcgatccc
     5041 gggatggcag catgcgcatc gatgtcgtga cgctgtttcc gcaaatgctg acgcaggcga
     5101 gcgcctatgg cgtcaccggg cgggcgcgcg agcgcgggct gtggcagctc ggtgtgtgga
     5161 atccgcgcga cttcgcaacc gatgcgtacc gcacgatcga tgatcgcccg tacggcggcg
     5221 gtcccggcat ggtgatgatg gccgcaccgc ttgcgcgcgc actcggtgcg gcgcgcgagg
     5281 cgcagcgcgc agcgggcgtc gcggcaagcc ggacgcttta tctgtcgccg gccggcactc
     5341 cgctgtcgca tcgcgcggtg atggatctcg ccggcgcgcc cggcgtggcg ctcacgcttc
     5401 tcgccggccg ctacgaaggc gtcgatcagc ggctgatcga tcgcgaggtc gacgccgagg
     5461 tatcgatcgg cgatttcgtc gtatcgggtg gcgaattgcc ggcgctgctg ctgatcgacg
     5521 cgatcgtccg ccagctgccc ggcgcgctga acgacgccga atccgcgcag caggattcgt
     5581 ttgccgacgg cctgctcgac tgtccgcact acacgcggcc cgagcatcac gacggtgaga
     5641 cggttccgcc cgtgttgctg tcgggcgacc acgcagcgat caggcgctgg cgtttgaagc
     5701 agtcgctcgg gcgcacatgg cagcggcggc ccgacctgct cggccggcgc gcgctgtcgc
     5761 atgaggagca ggcattgctg tcgcagtacc aggtcgaact aacggataat tgacgcgcgc
     5821 atcagggggc gccctgcgct gcacgcaaga ggcccctgcg gccaacgaga aaggacgtca
     5881 cgccatgaac atcatcgaaa cgctcgagcg cgaggagatc gcgcggctca acaagacgat
     5941 tcccgaattc gctcccggcg acacggtgat cgtcaacgtc aacgtcgtcg aaggcgagcg
     6001 ccggcgcgtg caggcgtacg agggcgtggt catcgccaag cgcaaccgtg gcctcaactc
     6061 ggcattcacc gtgcgcaaga tctcgagcgg cgagggcgtc gagcgcacgt tccagacgta
     6121 ttcgccgctg atcgcgtcga tcgaggtgaa gcgccgcggc gacgtgcgcc gggccaaact
     6181 ttactatttg cgcggccgtt cgggcaaatc ggcccggatt cgcgaaaagc tggcggtgcg
     6241 cgaaacggcc aagcccgagc agacgtcctg attcgcctgg cgggcaccgg gcactccgcg
     6301 cccggccgcg gcagtttgag aaggcggtcc ctcgggaccg ccttttttgt catctctgcc
     6361 atgcgcagcg cgcgcatcgc atggcccgga acttgcacac cgttgtcgcc caaatgtcca
     6421 acggcccgtc cccattgcgt ccggccgctt tttgcccgac tatcccgatg tcatatcatt
     6481 tgcgcgcctc accggtaacg cgatgaaggc cacgctgtac gacgcgctgg gcattcttcc
     6541 tacgtcgagc gacgaggaag tacgcgccgc gctgcgcggt ctcattcgca aatattatgc
     6601 gaagacgcgt gatggccagg gcaacgtcga agaagcgctg cgcttcatca atcatgcaag
     6661 tcgcatactg agcgacggcg agcggcgcga gcgctacgac gaggaactgt cgcgctcgac
     6721 gggcgacgag gacgatcccg cgcgcctcgc cgcgaagcgt gccgtgttcg gcgacgacgc
     6781 cgatagcgaa gcggggccac ccaccggcgt cggcgatgat gcaatcgcgg atccgctcga
     6841 ttccgagctg ccgggcgaca aggccgacgt tccggcaacg catcatcccg gactcaccga
     6901 gcgtgtcgcg tcgttcggcc ggtcgcgcct gctcacctac ggcctttgtg cgctcttcgg
     6961 cggattcatc gccaccgcga tcgtcttcgt cacgccgccg aacttcgtgg tcgtcgcgcg
     7021 ctcggtgctg ggctcgctcg tcgtggtgtt cctgctgctc gccgccgtct acgcgatcgt
     7081 gcacggcctc gggctcgttc gccgccgtcg catgggcacg cgctcggcgc tcgtcccgca
     7141 aaccgacctc gcgatcctga actggcgccg cgagcgcagc gtgttcctcg gcaccaacca
     7201 gccgcaggaa gacgcctcgt ggatcttcca gctgcgcatg gccgagctcg agcgcgcgaa
     7261 atccggacgc acgagcgagc cgcggccgtg gcatcggctc gcggcgcgca tgttcgatta
     7321 cgccatatgg ggcttgctgc tcgcgctttt gctgtcgcaa ctgcgcgctg ccgggctggt
     7381 gcccgaattg acggcgcact ggctcgcgca cccgctgatc gcacccattc tcatcacgct
     7441 gtccttcgtc ccgatcgagg cgctgctgat cacggcggtc ggcacgacgc cgggcaagtg
     7501 gctcttcggc atctatctgc agttctcgat ttcggatgcc tatgcccgcc gcgacacccg
     7561 catgcagctg ctgcgcgcgc tgttgcgcgc gtgtcgcgta tggttccagg ggctcggatg
     7621 cggctttcca ctgctcgccc cgatactcgt ggccgtcgcc cacgagcgcc tcgtgctgca
     7681 tcaggaaacc gactgggatt tcgccgagga ctgcctcgtc acgcaggggc ccgcgggtgt
     7741 cgtgaacatc gtgacgggtg tctgcggcct cgcatcgatg atgtggctct acggcgtcgc
     7801 atggcagcaa ccgatggccg atgcggtcgc atggacgaag gcttcggtca ttgccgcggt
     7861 gccgtcgcgc gacggtctcg agagtcgcct gggacaggcg agcggccgct tcgagcactt
     7921 cattcccgcg ttgtcgccgc gcagcgtatc cgatgcgctg cgcttgccct ggacgccggc
     7981 gccgtccgga accggaacgt cgacaaccgc gcccgcggca ttgcgcgaag tcgcgcccga
     8041 aacagggccg agcgatgccg agttcacgtc gatgatggcc gcgcggcggg cacgcatcga
     8101 ccttctcaag tcggaagggc cgcgtcttct acgcgcgggc aatgcacgtc gcgcgctcga
     8161 cgtctgccgc gcatgggtgg agctcgatct tgcaaattcc gaggcgtggc gctgcctcgg
     8221 acaggcgcag cttgccagcg gccagcatca ggaagcgctc aattcattcc gcaaggcacg
     8281 ccagcacaac ccgtcggatc gctcgctcga tgcggcgatc cagcaggccg agcgcagcat
     8341 cgtcgccgat ttccagcgtc gctacgggcg ctgatcgcgc ggcgacggga ccgaaaggcg
     8401 cgggttcggc cgcaccgtgc cgcgccgtgg cgtcagcggc gcttcagcac gaaccagaag
     8461 gcgccatcgt cctcgccgtg ctcgagcagc tcgttgccgg tctgccgcgc gaacgcctgg
     8521 aaatcgcgca cgctcgccgg gtcggtggcg gtaatgcgga tcatctggcc cgacgccatg
     8581 tcgttgagcg ccttcttcgt acgcaggatc ggcagcgggc agttgaggcc gcgcgcgtcg
     8641 atttcgcggt cgatcgtcag catggcagcg cgtttcgcat cgtgtgggga tacatgcaat
     8701 tatagcaacg catgcgctat gtcctcccgg tgctgctcgt cgtcgtcgtc gtcgccgttg
     8761 gcgcggcgtc cgtcgcgatc gatcgcgtcg acgtgccgcc gcgccggctc gggccctggg
     8821 tcgagcgcag ggcagaaggg caccacgaca tcctccagcg cggcgggcgg gagatctccg
     8881 ccgtgctcct tgcgctcgac cggggacgcg aggcattcca tgcgccgctg cctgttcccg
     8941 ccgtcggcga cggcgagccg ccgaccgcga agcgccgcgt gatcgtcgcc tccaccgagg
     9001 aggccttgca cgcgatagac gacgcccgac ccggtgatga catcaccttc gtccccggca
     9061 cgtaccggtt cgacggcgcc tacatcgatg cgcacgaagc gggcgcgccc ggcgcaccga
     9121 tcaccgtcag cgcggacgag ccgcacagcg tggtgctcga attcaacatg atcgagggct
     9181 ttctcgtgag cgcgccgtac tggtcgttcg cgaacctgac gatacgcggc gtttgcagcg
     9241 atcattcgaa ttgcgagcac gcattccatg tcgtgggagc ggccgggcat tttgtcgcgc
     9301 gagcaaacga gctcgtcgac ttcaatgcgc acttcaagat caacggaatg cgcggtgcgt
     9361 atcccgactg ggggctgatc gaaggcaacg tcatacgcaa cacgggcccg cgggaaacgg
     9421 ccaatcccgt gacgcccgtc gatctcgtcg ccgcgagtca ctggcgcatt cgcggcaatc
     9481 ggatcagcga tttcgtgaag cggcgcggtg acggcacgag ctacggcgcg ttcgtcaagg
     9541 gcggaggcag cgacaaccgc ctcgaggaca acgtcgtggt gtgcgaggac cgcttgcgcg
     9601 gctttccggg gcagcgcgtc gggctctcgc tcggaggggg tggatcgagc cgcgatgcat
     9661 gtcgtgacgg gcgctgcatc acggagcagg acgaaggcgt gatcgcgacg aacctgatcg
     9721 cctcatgctc cgacggcggc atctacctca accgcgccgc aacgagcgtc atcacgcaca
     9781 acacgctgat cgacacggcg ggtatcacgg cgcgctttgc cgagagcgcc gccgacgtcg
     9841 agggcaacct gatcgatggc ccgatacgcg ccgtcgacgg tgcgctgctg catcggaccg
     9901 acaatcgcat cacgggagtt gctccgctct acctggggtt gcatccggtg cgcgacctct
     9961 tcgtcgatgc gccgacgctc gacctccgtt ggcgcggcga tccgccgcgc cgccagcgcg
    10021 cggcatcgca accgccggac ctttgcggca cgccgcggcc ggccaggccc gtgtacggcg
    10081 cgttcgagga catcgcccgc tgtgcggcga gccgcaatta gcggcgcggt gacacgccct
    10141 aaccgcgaat gcgcttcacc agcgcgctcg tcgagcgctc gtagcgaaac gggatcgcga
    10201 cgaagcgccc gccgcgagcg atcacttccg ccgctccggc agtcgttgcg gccgaatagt
    10261 cgccaccctt gacgagcaca tcgggcgcgc actcgacgat caggtcgtgc ggcgtgtcgg
    10321 cgtcgaacgc gacgacgagg tcgacgctcg cgagcgcggc cagcaccgcc atccggtcat
    10381 cctggccgtt cagcgggcga tcggcgccct tgccgaggcg cttcgccgaa gcgtcggaat
    10441 tgacggcaac gacgagcgac gcgccgagcc ctttcgcctg ctcgagatac gtgacgtgac
    10501 cccgatgcag gatatcgaaa acgccgttgg tgaagaccag cgggcgtgcg agcgcgccaa
    10561 tgcgcgcgag cgcgtgcgcc gcatcggcga ttttgttcgg atagcggggc tcgggcagcg
    10621 acaccggcgg attcaccatc gggctcgctc gcatcgaagg gacgcgaagc gttgttgcgc
    10681 tagtcgatgt actcgaacag cttgacgaca cgcacgacgc ccgacgtcgt cgcggcgatc
    10741 tgtgccgccg catcggcctc ggctcggcgt acgaggccca tcaggtagac cacctgccgc
    10801 tcggtgacga ccttcacatg attgggcgcg aacttgttcg cctcgacgaa gcgcgccttg
    10861 accttggacg tgatgtacga atcgttcgtg cgcgagccga tgctcgacac cggaccgacg
    10921 ctgagctcgt ccgtcacgct gcgcaccttc gcggtcgact tcgcgatttc ctcgatcgag
    10981 cgcttgagct cgggcgaggg gacctcgccg gtcagcagaa cgatgccgtt gtagctcgtg
    11041 acgttcacat gcacgcggtc gccgtagccc gcgccgatgt tgttcgccat cttgagctcg
    11101 atcgcttcat cgtcggtctg cgcgcccacc gagcgccggt cggcggcgac cagtgccgcg
    11161 ccaccggccg ccccggcgat caccagcggc acgcaaccgc tgagcacgat cgaaccgcag
    11221 gccaggaacg cgaacgcggc gaagatcagg cgcttcctca tgcatcgtct cccaaaagcg
    11281 tggcgtcgac gacgtcgcac aggcaatgaa tcgtcaacag gtggacttcc tggatgcgca
    11341 tcgtgcgcgc gtgcggaacg cacaagtgga cgtcttcggg cgcgagaagt tcgccgatgc
    11401 gtccgccgcc cttgccggtc agcgccacga cgcgcatttc gcgctcgtgc gcggcaacga
    11461 cggccgcgat cacgttcgca gaattgcccg atgtggagat cgcgagcagc acgtcgcctt
    11521 tggcgccgag cgcgcgcacc tgcttcgcga aaatctgctc gaacgaataa tcgttggcga
    11581 tcgccgtcag cagcgacgaa tccgtggtga gcgcgatagc gggcagttcc ggacgctcgc
    11641 gctcgaaccg gcccacgagc tcggccgcga agtgctgcgc gtcagccgcc gatccgccgt
    11701 tgccgcacgc gaggatcttg ccgtcggcga acaggcaatc ggtcagcacg gcaccggcgc
    11761 gcgcgatcgg cgccgccatc gtttgcgccg ccgcctgctt cagtgccgcg ctgtcgtcga
    11821 agtggccgcg gatgcgctcg agatgatcga tcatggccgg cgattctaac agtgtccccc
    11881 cacgcttgcc gcttgcgcgg cggcgctgcc cccaaggggg cgcaattcgc gccttggggc
    11941 ggccctgcgg cgcgaatgtc agccgtcgac tgcgatcacg tcgcggcgcc attcgatcgt
    12001 cgccgcgtcg agcgagcgca tcaggatcgc gtcgaagcgg cacggcggct cgcggccgag
    12061 catcgcaagg tagccgttcg ccgcggcgac gaggcgcgcg cgcttgcgcg gcgtgatgct
    12121 ctcgacggca ccgccgtaac gcgtcgagcg gcgcagccgc acttcgacga acaccagcac
    12181 gcgcccgtca cgcgcaatga gatcgatctc gccgaagcgc gtgcggaagt tgcgcgcgac
    12241 gatcgcgaga ccctgctgca gcaggaacgc tgccgcgagc gcctcggcgc ggccgcccgc
    12301 cgtgatcaag gcgtcgaatc gatcggcacg agcgcgccat cgcggtagac gccgagcacg
    12361 ccctcgcgct ggaactcgtg gcccgggccc agcgtgatat ggccggttgc gccatcgacg
    12421 tcgaaatgcg ccggtggc
//
LOCUS       DASSVX010000334         3262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_498046, whole genome shotgun sequence.
ACCESSION   DASSVX010000334 DASSVX010000000
VERSION     DASSVX010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498046"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFO53_14325"
     CDS             complement(<1..304)
                     /locus_tag="VFO53_14325"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET8877501.1"
                     /translation="MNTIFIHDLRLDTRVGVYAWERELPQTIRVDLDIGLPSSRPFET
                     GELADAIDYAAVVKRLRVFAADHPHALLERFAEAVASVVLDEFGAQSVKLRVAKLGA"
     gene            361..960
                     /gene="plsY"
                     /locus_tag="VFO53_14330"
     CDS             361..960
                     /gene="plsY"
                     /locus_tag="VFO53_14330"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET8877502.1"
                     /translation="MAATLLLIAGAYLLGSVSFAVVVSRAFGLPDPHGYGSGNPGATN
                     VLRTGHRGAALLTLLGDGAKGFVAVFAARHLAPLLGAPDVAVAAAALAVFLGHLYPVF
                     HRFAGGKGVSTAAGIVLALYWPLGVALTAVWLTMALGFKISSLAALTTAVLLPLGLFY
                     VRGPGAEATVGVIIALLLFWRHRTNIRELRSGRERTIGR"
     gene            complement(971..1999)
                     /gene="tsaD"
                     /locus_tag="VFO53_14335"
     CDS             complement(971..1999)
                     /gene="tsaD"
                     /locus_tag="VFO53_14335"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET8877503.1"
                     /translation="MRVLGIETSCDETGVALYDTERGLVAEALHSQVDLHAAYGGVVP
                     ELASRDHVRRVLPLARDVLTRAGLAVHEVDAIAYTRGPGLAGALLVGASVANALGFAL
                     GKPVLGIHHLEGHLLSPLLGHPAPAFPFVALLVSGGHTQFFEVRGVGGYRLLGDTQDD
                     AAGEAFDKTAKLLGLAYPGGPALAALAEQGNPHAVALPRPMIDSGDLDMSFSGLKTAV
                     ALRAREAAGGGDASQLPVSRKADIAAAFQAAVVDVLVAKSLRALDITGMSSLVVAGGV
                     GANRLLRMRLGEALATRGARAWFPEARYCTDNGAMIAMAAALRLGDARRDYRFSVEPR
                     WELRTLGR"
     gene            2097..2309
                     /gene="rpsU"
                     /locus_tag="VFO53_14340"
     CDS             2097..2309
                     /gene="rpsU"
                     /locus_tag="VFO53_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET8877504.1"
                     /translation="MTSVRVRENEPFEAALRRFKRTIEKTGLLTELRAREFYEKPTSE
                     RKRKKAAAVKRHYKRLRSQQLPPKLY"
     gene            2379..2828
                     /locus_tag="VFO53_14345"
     CDS             2379..2828
                     /locus_tag="VFO53_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET8877505.1"
                     /translation="MALKDRITDDMKDAMRARAAARLSTIRLLLAAIKQREVDERKAL
                     TDADIVAVVDRMIKQRKDSITQFDAGHRPDLADAERAELAILETYLPQRMTDAEIDAA
                     IDAALSAARAAGASGPALMGKAMAALKPQLTGRADMAQVSARVKARL"
     gene            2825..>3262
                     /locus_tag="VFO53_14350"
     CDS             2825..>3262
                     /locus_tag="VFO53_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HET8877506.1"
                     /translation="MIPRDFIETLLARVDIIEVIDRSVPLKKAGANYQACCPFHSEKT
                     PSFTVSPTKQFYHCFGCGAHGTAIGFLMEHTGQSFPEAVETLARDAGLEVPRTERPGE
                     REERKVSQDLSETLLAAAKFYRAQLKDAPPAIEYLKRRGLTGVV"
BASE COUNT          544 a         1123 c         1094 g          501 t
ORIGIN      
        1 gtgcgccgag cttggcgaca cgaagcttca ccgactgcgc accgaattcg tcgagcacga
       61 ccgacgcgac tgcttcggcg aagcgctcga gcagcgcatg cggatgatcg gccgcgaaga
      121 cgcgcagccg cttcacgacc gcggcgtagt cgatcgcgtc ggccagctcg cccgtttcga
      181 acggacgcga cgacggcagg ccgatgtcga gatcgacgcg aatcgtctgc ggcaattccc
      241 gttcccacgc gtagacaccg accctggtgt cgaggcgcag gtcatggatg aagatcgtgt
      301 tcatcgactg atccgggcta gaatcgcgaa gccatcttac cgaaagcgcg cgatcctgcc
      361 gtggctgcaa cgcttctcct gatcgccggc gcgtatctgc tcggttcggt gtcgttcgcc
      421 gtggtggtga gccgcgcctt cggcctgccg gatccgcatg gatacggttc gggcaatccc
      481 ggagcgacga acgtgcttcg cacgggtcat cgcggcgcgg cgctgctcac gttgctcggc
      541 gacggcgcga agggattcgt cgcggtgttc gcggcccgcc atctcgcacc gctcctcggt
      601 gcgcccgacg tggcggtggc tgcagcggcg ctggccgttt tcctcgggca cctgtatccg
      661 gtgtttcatc gttttgccgg cggcaagggt gtgtcgacgg cggcgggtat cgtgcttgca
      721 ctgtactggc cgctcggtgt cgcgctgacg gccgtgtggc tgacgatggc gctcggcttc
      781 aagatcagct cgctcgccgc gctcacgacg gccgtgctgc tgccgctcgg gcttttctat
      841 gtgcgcgggc ccggtgccga agcgacggtc ggcgtcatca tcgcgctgct gctcttctgg
      901 cgccatcgca cgaacatccg cgagttgcga agcggccgcg aacgcacgat cggaagataa
      961 gggcgtgagc tcatcggcca agcgtgcgca gctcccagcg cggctcgacc gagaagcgat
     1021 aatcgcgacg cgcgtcgcca aggcgcagcg cggctgccat ggcgatcatc gcgccgttgt
     1081 cggtgcagta gcgcgcctcg ggaaaccagg cgcgcgcacc ccgtgtcgcg agcgcctcgc
     1141 ccaggcgcat tcgcaggagc cggttcgccc ccacgccgcc ggcgacgacg agcgacgaca
     1201 tgccggtgat gtcgagcgcg cgcaacgact tcgcgacgag cacgtcgacg accgccgcct
     1261 ggaacgccgc cgcaatgtcc gccttgcgcg ataccggcaa ctgcgacgca tcgccgccgc
     1321 cggccgcttc gcgcgcacgg agcgcgacgg cggtcttgag accggagaag ctcatgtcga
     1381 ggtcgcccga gtcgatcatc ggccgcggca gcgcaaccgc gtgcgggttg ccttgctcgg
     1441 cgagggctgc gagcgcggga ccgcccggat acgcaagtcc gagcagtttc gcggtcttgt
     1501 cgaacgcctc gccggctgcg tcgtcctgcg tgtcgccgag caaccgatag ccgcccacgc
     1561 cgcgcacctc gaagaactgc gtgtggccgc ccgacaccag cagcgcgacg aacgggaatg
     1621 ccggtgcggg atgacccagg agcggcgaca gcagatgccc ttcgaggtgg tgaatgccga
     1681 gcaccggctt gccgagcgca aagcccagcg catttgcaac actcgcaccg acgaggagcg
     1741 cgccggccag tcccggtccc cgcgtgtagg cgatcgcgtc cacctcgtgc acggcaaggc
     1801 cggcgcgtgt cagcacgtcc ctcgcaagcg gcagcacgcg gcgcacgtga tcgcgcgagg
     1861 cgagctccgg cacgacgccg ccgtaggccg cgtgcagatc gacctgcgag tgcagcgcct
     1921 cggcgacgag gccgcgctcg gtgtcgtaga gcgcgacacc ggtctcgtcg caggaggttt
     1981 cgatgccgag aacgcgcatg ggccactata ccgcgcgctt gcagcacgcg gctcgcggct
     2041 gctataatcc ccggttaccg taacctcttg aatgcacgga aaggtaccac cccaacatga
     2101 ccagcgttcg cgttcgcgag aacgagccct tcgaggcggc gctacgccgt ttcaagcgca
     2161 ccatcgagaa aacgggactc ctcaccgagc ttcgggcccg cgagttctac gaaaagccca
     2221 cctcggagcg caagcgcaag aaggccgccg cggtgaagcg gcactacaag cgtcttcgca
     2281 gccagcagct gccccccaag ctctactagg gttgcgcgcg cctcgcgcgc gaattttcga
     2341 ctgaccaggg gcgatgcgtt tcgccccttt ttgcttccat ggccctcaag gatcgcatca
     2401 ccgacgacat gaaggacgcg atgcgggcga gggccgccgc gcgtctgtcg accatccggc
     2461 tcctgctggc cgcaatcaag cagcgtgagg tcgacgagcg aaaggcgctg accgacgccg
     2521 atatcgttgc cgtcgtcgac aggatgatca agcagcgcaa ggattccatc acgcagttcg
     2581 acgccggcca ccggcccgat cttgcggatg ccgagcgcgc ggagctcgcc atactcgaaa
     2641 cgtacctgcc gcagcggatg accgacgccg aaatcgacgc cgcgattgat gccgcgcttt
     2701 ccgctgcgcg tgcggccggc gcgtcggggc ccgcgctgat gggcaaggcg atggctgcct
     2761 tgaagccgca actcaccggg cgcgccgaca tggcgcaagt ctcggccagg gtgaaagcgc
     2821 gcctctgatt ccccgcgact tcatcgagac gctcctcgcg cgcgtcgaca tcatcgaggt
     2881 gatcgaccgc tcggtgccgc tcaagaaagc gggcgccaac taccaggcgt gctgcccgtt
     2941 ccacagcgag aagacgccgt cgttcacggt gagccccacc aagcagttct accactgctt
     3001 cggatgcggc gcgcacggca ccgcgatcgg cttcctgatg gagcacaccg ggcagtcgtt
     3061 ccccgaggcg gtcgaaacgc tcgcgcgcga cgcggggctc gaggtgccgc gtaccgagcg
     3121 ccccggcgag cgcgaagagc gcaaggtgtc gcaggacctc tccgaaacgc tgctcgccgc
     3181 ggcaaagttc taccgcgcac agctcaagga tgcgccaccg gcgatcgagt atctgaagcg
     3241 gcgcgggctg accggcgtgg tg
//
LOCUS       DASSVX010000335         6825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_664868, whole genome shotgun sequence.
ACCESSION   DASSVX010000335 DASSVX010000000
VERSION     DASSVX010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664868"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFO53_14355"
     CDS             <1..393
                     /locus_tag="VFO53_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HET8877507.1"
                     /translation="LLHDACYAFPGHIIVGLDARDGKVATDGWSKMTGHDVVDLAKKF
                     EDYGVEAIVYTDIGRDGMLTGVNIEATVKLARELKTPVIASGGIASLDDIHQLVPYAG
                     DGIVGAIAGRALYEGRLDFKEALKAAKG"
     gene            393..1145
                     /gene="hisF"
                     /locus_tag="VFO53_14360"
                     /pseudo
     CDS             393..1145
                     /gene="hisF"
                     /locus_tag="VFO53_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
     gene            1250..1657
                     /gene="hisI"
                     /locus_tag="VFO53_14365"
     CDS             1250..1657
                     /gene="hisI"
                     /locus_tag="VFO53_14365"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HET8877508.1"
                     /translation="MTGAPRSDDWLDAVRWNRDGLAPAIAQDAATHRVLTLAWMNREA
                     LTLTRDTHEAHYWSRSRHKLWRKGEQSGHVQRVREIRLDCDRDAILLVVEQVGGVACH
                     TGRERCFYRRLDGDQWQTIEPILEDPITVYGRE"
     gene            1644..2006
                     /locus_tag="VFO53_14370"
     CDS             1644..2006
                     /locus_tag="VFO53_14370"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HET8877509.1"
                     /translation="MAANDAPGTAASDAPAADVLARVAATIEARKRADPQASYVASLL
                     GAGDDRILKKIGEEATETVLAAKDNDKIRLVAEVADLWFHCLVLLARHDLGPADVLRE
                     LARREGISGHAEKASRGS"
     gene            2043..2414
                     /locus_tag="VFO53_14375"
     CDS             2043..2414
                     /locus_tag="VFO53_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HET8877510.1"
                     /translation="MASDSNCIFCKIARGELPSRKVYEDADVFAFHDIQPVAPVHFML
                     VPKKHIATLYDATADDAPVLGRILELAGRLAREQGAGDGFRTIINTGRVGRQDVKHVH
                     VHVIGGPDPLGPMMGPAAASG"
     gene            2428..2658
                     /gene="tatA"
                     /locus_tag="VFO53_14380"
     CDS             2428..2658
                     /gene="tatA"
                     /locus_tag="VFO53_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HET8877511.1"
                     /translation="MGSFSIWHWMIVLLVVVLIFGTKKLRNIGQDLGGAVKGFKEGMK
                     SAEEPAAPEEPPAQVTGRTIEGEVRQERPTKV"
     gene            2718..3173
                     /gene="tatB"
                     /locus_tag="VFO53_14385"
     CDS             2718..3173
                     /gene="tatB"
                     /locus_tag="VFO53_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HET8877512.1"
                     /translation="MFDIGFSEIVVIGVVALVVIGPERLPKTARTVGLLFGRLQRYVN
                     DVKADISREMELDELRRLQSEMQGAARDLESTVKGAANDMAAGVRSVESELNAAASQA
                     TETGGTAVSSPPLPVAAAGIDAAPPLPVSSAGTEAPPPRQAPLPGFDKI"
     gene            3221..3985
                     /gene="tatC"
                     /locus_tag="VFO53_14390"
     CDS             3221..3985
                     /gene="tatC"
                     /locus_tag="VFO53_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET8877513.1"
                     /translation="MSETSEAQESFLSHLIELRTRLMRSIIAVVVVLLVLFPFAKDIY
                     ALLAQPLLRVLPHGSTMIATDVTGTFLVPLKVTLMAAFLIALPYVLWQAWAFVAPGLY
                     QHEKRLVMPVLVSSCLFFLFGMSFAYFFVFPVAFGFFAGYAPVGVQMMTDIDKYLSFV
                     LTMFMAFGITFETPVIVVVLVRMRVVTLEKLRSFRPYVIVGAFVVGAVFTPPDVISQS
                     LLAVPLWLLYELGLVLARFVSTAAPEAAAEETEPAQ"
     gene            complement(4030..5184)
                     /locus_tag="VFO53_14395"
     CDS             complement(4030..5184)
                     /locus_tag="VFO53_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET8877514.1"
                     /translation="MFKKFWLLFAQACTLCVAALFVVATLRPDLLPRLSGKSSNVVLL
                     QQSRAQAAAPGATSYADAAKKAMPAVVNIYTSKAVRARNPLLDDAIVQRYFPELARRT
                     PARQTTSLGSGVIVAPEGYVLTNHHVVEGADDIELVLADGRELHARVRGVDPESDLAV
                     LKADGENLPTITFATMEDLHVGDVVLAIGNPFGFGNTVTMGIVSALGRNHLGVNRFED
                     FIQTDAPINPGNSGGALVDTAGNLIGINSTIFSQSGGSMGIGFAIPVSLARTVLAQII
                     KEGEVTRGWLGVEPQPITREAAQGLALAHTDGVLVRALQRDGPADRAGIQVRDIVVEI
                     GGKRTPDVPQLLARIAELTPGSNARVKVMRDGKPLDIDVTVAKRPRAETP"
     gene            5416..6009
                     /gene="petA"
                     /locus_tag="VFO53_14400"
     CDS             5416..6009
                     /gene="petA"
                     /locus_tag="VFO53_14400"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HET8877515.1"
                     /translation="MGTEAIANKDKRRFLIGATSVVAGVGAVAVATPFVMSLWPSERA
                     KAAGAPVEIDISKIEPGQKINVEWRGKVCWLVNRTAPMLATLPKMDPRVADPKSEVPQ
                     QPPYCKNETRSIKPALWVAVGICTHLGCSPTYRPEVAPADLGEDWLGGFFCPCHQSKF
                     DLAGRVLKGVPAPTNLVVPPHKYLTDTRVVIGEDTKS"
     gene            6023..>6825
                     /locus_tag="VFO53_14405"
     CDS             6023..>6825
                     /locus_tag="VFO53_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HET8877516.1"
                     /translation="MSKLLTWIDDRFPLTALWNSQWGKYVAPKNFNFWYYFGSLAALV
                     LVMQILTGIWLTMNYKPDATKAFESIEYIMRDVQWGWLIRYMHSVGASMFFVVVYLHM
                     FRGLMYGSYRKPRELTWVFGCLIYLALMAEAFFGYLLPWGQMSYWGAQVIVNLFSTIP
                     VIGDDLSVWIRGDYTISDITLNRFFAFHVAALPLVLIALVAAHFMALHETGSNNPDGV
                     EIKKQPKDPNTGLPLDGIYSHPYYTIKDIVGVIVFLAVFSIIVFFLPEMG"
BASE COUNT         1110 a         2232 c         2328 g         1155 t
ORIGIN      
        1 ctcctgcacg acgcgtgcta cgcgtttccc ggccacatca tcgtcggcct cgatgcgcgc
       61 gacggcaagg tcgcgaccga tggctggtcg aagatgacgg gtcacgacgt cgtcgacctc
      121 gcgaagaagt tcgaggacta cggcgtcgag gcgatcgtct ataccgacat cggccgcgac
      181 ggcatgctga ccggcgtcaa catcgaggct accgtcaagc tcgcgcgcga gctcaagacg
      241 ccggtgatcg cgagcggtgg catcgcttcg ctcgacgata tccaccagct cgttccgtac
      301 gcgggcgacg gaatcgtcgg cgccatcgcc gggcgggcgc tctacgaggg acgactcgat
      361 ttcaaggaag cgctgaaggc ggcgaagggc tagtggtcgc caagcgcatc attccctgcc
      421 tcgacatcgc cggcggacgc gtcgtcaagg gcgtcaattt cgtcgagttg cgtgacgcgg
      481 gcgatcccgt cgagatcgcg cgccgctacg acgagcaggg cgccgacgag ctcgcgttcc
      541 tcgacattcg cgcgagcgtc gagaatcgcg cgctcctcta cgacatgatc gaggcggtcg
      601 ccgaccaggt gttcatcccg ctcaccgtcg gcgggggcgt ccagtcggtc gacgacgttc
      661 gcgcactgct caacgccggc gcggacaagg tgagcatcaa cacggccggc gtgcagcggc
      721 ccgagctcta cgccgaggcg tcggcgcgct tcggtgcgca atgcatcgtc gccgcgatcg
      781 acgtgaagca gtgcgccgtc gacgagtggc aggtgtacat ccatggcggc cgcaccccga
      841 ccggcatcga cgccgtcggc tgggccgagc aggtggcggg actcggcgcg ggcgaaatac
      901 tgttgacgag catggaccgc gatggcgtgc gcaagggctt cgaccttcgc gtcacgcgcg
      961 aggtcgccga ccgcgtgaac gtgccggtca tcgcgtcggg cggcgtgggc tcgctgcagg
     1021 atctcgtcga ggggattgtc gttggcggcg ccgacgcggt cctcgccgcc tcggtctttc
     1081 actacggcaa gtacacggtg ggcgaggcga aggcggcgat gcgcgccgcg gggatcgatg
     1141 tacgagagag caccgcgggc gaagccgagg cccgaagggc acggcgcggt gcgaccgagg
     1201 cggccgacac gttctctcgc gcaacgcaag gcagcacaaa gaggccgcga tgactggcgc
     1261 gccgcgcagc gacgactggc tcgacgccgt gcgctggaac cgcgacgggc tcgcgccggc
     1321 catcgcgcag gacgccgcga cgcaccgggt gctgacgctt gcatggatga accgcgaggc
     1381 gctcacgctc acccgcgata cgcacgaggc gcactactgg tcgcgctcgc gccacaagct
     1441 gtggcgcaag ggcgagcaat cgggccatgt gcagcgcgtg cgcgagatac ggctcgattg
     1501 cgaccgcgac gcgatcctgc tggtcgtcga gcaggttggc ggcgtcgcct gtcacacggg
     1561 tcgcgagcgc tgcttctatc ggcgtctcga tggcgaccag tggcaaacga tcgagccgat
     1621 cctcgaagac ccgatcaccg tctatggccg cgaatgacgc gcccggcacg gccgcgagtg
     1681 acgcgcccgc cgcggacgtg ctcgcgcgcg tggccgccac gatcgaggcg cgcaagcgcg
     1741 cggacccgca ggcatcgtac gtcgcatcgc tgctcggcgc cggcgacgac cgcatcctga
     1801 agaagatcgg cgaggaagcg accgagacgg tgctcgcggc gaaggacaat gataaaattc
     1861 gtctggttgc cgaggtggcc gatctatggt tccactgcct cgtgctgctc gcccgccacg
     1921 atctcggtcc cgcagacgtg ctgcgtgaac tggcgcggcg ggaaggcatt tccggccacg
     1981 ccgagaaggc gtcgcgcgga agctgaactt gcgccggcac ccgcggcgcg cgaacgatcc
     2041 ccatggcgag cgactccaac tgcatatttt gcaagatcgc gcgcggcgag ttgccgagcc
     2101 gcaaggtgta cgaggacgcc gacgtgttcg cgttccacga catacagccg gtcgcgccgg
     2161 tgcacttcat gctggtgccc aaaaagcaca ttgcaacgct gtacgacgca acggccgacg
     2221 acgcgccggt gctcggcagg attctcgagc tggccggccg gctcgcgcgg gagcagggtg
     2281 ccggcgacgg cttccggacg atcatcaaca ccggccgtgt cgggcgccag gacgtgaagc
     2341 acgtccacgt gcacgtgatc ggcgggccgg acccgctcgg tcccatgatg gggccggcgg
     2401 cggcatcagg ataacgagga gggaacgatg ggcagtttca gcatctggca ctggatgatc
     2461 gtattgctcg tggtcgtgct gatcttcggc acgaagaagc tgcgcaacat cgggcaggac
     2521 ctgggcggcg cggtcaaggg cttcaaggaa ggcatgaaaa gcgccgagga accggctgcg
     2581 cccgaggagc cgcccgcgca ggtcaccgga cgcacgatcg agggcgaggt ccgccaggag
     2641 cggcccacca aggtatgacg gcggcgggga tgcggatacg ctcgcgggcc cggcacgccg
     2701 gccgctagcg gttcgccatg ttcgacatcg gcttttccga aatcgtcgtc atcggcgtcg
     2761 ttgcgctcgt cgtcatcggg cccgagcgcc tgccgaaaac cgcgcgcacg gtcggcctgc
     2821 tgttcgggcg gctgcaacgg tacgtgaacg acgtgaaggc cgacataagc cgcgaaatgg
     2881 agctcgacga actgcgccgc ctgcaaagcg aaatgcaggg ggccgcgcgc gatctcgagt
     2941 cgacggtgaa gggcgcggcc aacgacatgg ccgccggcgt tcgcagcgtc gagagcgagc
     3001 tcaacgccgc ggcgtcgcag gccacggaga cgggcggcac ggccgtgtcg tcgccgccgc
     3061 ttccggtggc cgcggcaggc attgacgcgg cgccgccgct tcctgtgtcc tccgcgggca
     3121 ccgaggcgcc gccgccgcgt caggccccct tgccgggctt cgacaagatt tgatttcgcg
     3181 ctggcgttcg cgtcggcatt cgacggccgc cccctctcca atgagcgaaa cctcggaagc
     3241 ccaggagtcc ttcctttcgc acctgatcga gctgcgcacg cggctgatgc gctcgatcat
     3301 tgcggtcgtc gtcgtgctgc tcgtgctgtt cccgtttgcc aaggacatct acgcgctgct
     3361 cgcgcagccg ctgttgcgcg tgcttccgca cggctcgacg atgatcgcca ccgacgtcac
     3421 cggcaccttc ctcgtgcccc tcaaggtgac gctaatggcg gcgttcctca tcgcgctgcc
     3481 gtacgtgctg tggcaggcat gggcgttcgt cgccccgggg ctgtaccagc acgagaagcg
     3541 gctggtgatg ccggtgctcg tgtcgagctg cctgttcttc ctgttcggga tgtcgttcgc
     3601 gtacttcttc gtcttcccgg tcgcgttcgg ctttttcgcc ggttacgcgc cggtcggcgt
     3661 gcagatgatg accgacatcg acaagtacct gtcgttcgtg ctgacgatgt tcatggcgtt
     3721 cgggatcacg ttcgagacac ccgtcatcgt cgtcgtcctc gtgcgcatgc gtgtggtgac
     3781 cctcgagaag ctgcgctcgt ttcgcccgta cgtgatcgtc ggcgcgttcg tcgtcggtgc
     3841 ggtgttcacg ccgcccgacg tgatctcgca aagcctgctc gcggtgccgc tgtggctgct
     3901 ctacgagctc gggctcgtgc tggcgcggtt cgtatcgacg gcggcgccgg aagcggctgc
     3961 agaggaaacc gagccggcgc agtaagcggg ttcagtaagc gggttcaaat ggcgtgcgcg
     4021 gctcgttcgc tacggcgttt ccgctcgcgg ccgtttcgcc accgtcacgt cgatgtcgag
     4081 cggcttgccg tcgcgcatta ccttcacccg cgcgttcgat ccgggcgtga gctccgcgat
     4141 cctcgccagc agttgcggca cgtcgggagt gcgcttgccg ccgatttcga ccacgatgtc
     4201 gcgcacctgg atgccggcac gatcggccgg cccgtcacgc tgcagcgcgc gcaccagcac
     4261 gccgtcggta tgcgcaaggg cgagcccctg cgcggcctcg cgggtgatcg gctgcggctc
     4321 gacgccgagc catccgcgcg tgacttcgcc ttccttgatg atctgcgcga gcaccgtgcg
     4381 cgcgagcgag accggaatgg cgaagccgat gcccatcgat ccgcccgact gcgaaaagat
     4441 cgtgctgttg atcccgatca ggttgccggc ggtatcgacg agcgcgccac ccgagttgcc
     4501 cgggttgatc ggcgcatcgg tctggatgaa gtcctcgaag cggttgacgc ccagatgatt
     4561 gcggccgagg gcgctcacga tgcccatcgt cacggtattg ccgaagccga acgggttgcc
     4621 gatcgcgagc acgacatcgc cgacgtgcag gtcctccatg gtggcgaacg tgatcgtcgg
     4681 caggttctcg ccatcggcct tcagcaccgc gaggtccgat tcgggatcga cgccgcgcac
     4741 gcgggcatgc agctcgcgtc cgtcggcgag gacgagctcg atgtcgtcgg cgccttcgac
     4801 gacgtggtgg ttggtgagca cgtacccctc gggggcgacg atgacgccgg agccgaggct
     4861 cgtggtctgc cgcgcggggg tgcgtcgcgc gagctcgggg aagtagcgct gcacgatcgc
     4921 atcgtcgagc agcgggttgc gcgcgcggac cgccttgctc gtatagatgt tgacgacggc
     4981 gggcatcgcc ttcttcgccg catcggcata gctggtcgcg ccaggcgccg cggcctgggc
     5041 gcgcgattgc tgcagcagga cgacgttgct cgatttgccg gacaggcgcg gcaacaggtc
     5101 ggggcgaagc gtcgccacga cgaagagcgc ggcgacgcac agcgtgcacg cctgcgcaaa
     5161 caggagccag aatttcttga acacgtgtcg atcggtcaac gacgtcgatt atagtcgcgc
     5221 gcacgcggcg tcgcattgcc cggatgcacg ccggccggcg tgcatccggc acgatgatcc
     5281 gatgcaaacc cttgttcctg cagcgcaaag cgctggaaat tccgcgatgc gctgcaaccg
     5341 gaacggcggc ttcccgtata attccaaggc tttgcgggac gctcttcgtt actcgcacgc
     5401 ggacgaaagg cagggatggg gacagaagcg atagcaaaca aggacaagcg ccgcttcctg
     5461 atcggcgcca catcggtggt cgccggggta ggggcggtcg ccgtcgcgac gcccttcgtc
     5521 atgtccctct ggccgagcga gcgggcgaaa gccgccggag cgcccgtcga aatcgacatc
     5581 agcaagatcg agccgggcca gaagatcaac gtcgaatggc gcgggaaggt gtgctggctc
     5641 gtcaaccgca ccgcgccgat gctggcgacg ctgccgaaga tggatccgcg cgtcgccgat
     5701 ccgaagtccg aggtcccgca gcagccgcct tactgcaaga acgagacgcg ctcgatcaag
     5761 cccgcgctgt gggtcgccgt cggcatctgc acgcaccttg gctgctcacc gacctaccgc
     5821 ccggaagtcg cgccggccga cctcggcgag gattggctcg gcggcttctt ctgcccgtgc
     5881 caccagtcga agttcgatct cgcgggacgc gtgctgaagg gcgttcccgc gccgaccaac
     5941 ctggtcgttc cgccgcacaa gtacctgacc gacacccgcg tcgtgatcgg ggaagacacc
     6001 aagtcgtgaa aggccccgtg cgatgagcaa gttgctgacc tggatcgacg accgctttcc
     6061 gctgacggcg ctttggaaca gtcagtgggg caagtacgtc gcgccgaaga atttcaattt
     6121 ctggtactac ttcggctcgc tcgccgcgct ggtgctcgtg atgcagatcc tcaccggcat
     6181 ctggctcacg atgaactaca agcccgatgc gacgaaggca ttcgagtcca tcgagtacat
     6241 catgcgcgac gtccagtggg gctggctgat ccgctacatg cattcggtcg gcgcgtcgat
     6301 gttcttcgtc gtcgtgtacc tgcacatgtt ccgcggactg atgtacggca gctaccgcaa
     6361 gccgcgcgag ctcacgtggg tcttcggctg cctcatctac ctcgcgctga tggcggaggc
     6421 gttcttcggc tacctgcttc cgtgggggca gatgtcgtac tggggcgcgc aggtgatcgt
     6481 caacctgttc tcgacgatcc cggtgatcgg cgacgacctg tcggtctgga ttcgcggcga
     6541 ctacacgatc tccgacatca cgctcaatcg cttcttcgcg ttccatgtgg ccgcgctgcc
     6601 gctggtgctc atcgcgctgg tcgccgcgca cttcatggcg ctgcacgaga ccgggtcgaa
     6661 caaccccgac ggcgtcgaga tcaagaagca gccgaaggac ccgaacaccg gattgccgct
     6721 cgacggcatc tattcgcacc cgtactacac gatcaaggat atcgtcggcg tcatcgtctt
     6781 ccttgccgtc ttctcgatca tcgtgttctt cctgcccgag atggg
//
LOCUS       DASSVX010000336        13534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_115028, whole genome shotgun sequence.
ACCESSION   DASSVX010000336 DASSVX010000000
VERSION     DASSVX010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 13534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13534
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115028"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFO53_14410"
     CDS             <1..528
                     /locus_tag="VFO53_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET8877517.1"
                     /translation="DLSNYVGFGNHEVQLLNIRTGTFDTYTISSPTNSSGKVKGVELA
                     YQQELAYGFGVQANYTYADAHETGGRDLVGASRNTYNVIGYWEGHGFSARLAYTFRSH
                     FFVGLDRSTPEYQDDTGTLAASLSYAINDNVALTFDALNLNNPTLKYYGANTDQPRAF
                     YKNGRQFYAGVRVKF"
     gene            667..1725
                     /locus_tag="VFO53_14415"
     CDS             667..1725
                     /locus_tag="VFO53_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018557232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET8877518.1"
                     /translation="MTALACALTIFTAGCVAPRAGSGAAASSTPSAAPASEAARFVYS
                     PYKYVPVGWDADAAAIATTAGGRVVPLAADARSTLPAGVVAVTLAFATGECGAETWDG
                     VDPDVLARVNVRRFERAGIDYIVSTGGEAGTFSCATDAGMERFIARYASSRLIGLDFD
                     IERGQSAELLASLVRRIKAAKDRHAGLRISFTLATWASSDGSGASLNADGERVMAAIR
                     GAGLDDFYVNLMAMDYGTASARNCVVVDGVCDMGRSAMQAARNVAARFAVPLARIELT
                     PMIGVNDVMTNVFTLDDAAALGGFVREAGLGGLHFWALDRDTPCPHGAAIVLSACSGL
                     DAAPYAFTRAFARALQAH"
     gene            1762..2031
                     /locus_tag="VFO53_14420"
     CDS             1762..2031
                     /locus_tag="VFO53_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877519.1"
                     /translation="MRALALSVSMILLVAGCATPAQRAAQVQRDVDEMVAVYGPGCER
                     LGYKADSDPWRECVLHLATKDRLERRDFTTTNCIGSRGFFQCSTF"
     gene            complement(2050..3102)
                     /locus_tag="VFO53_14425"
     CDS             complement(2050..3102)
                     /locus_tag="VFO53_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HET8877520.1"
                     /translation="MNNDTIADAPAAVQPAQIAEWHGVDAAIFRDEIVPRYRPAVLRG
                     LVADWPAVASGRQSPQAVAHYLARFGSAHAVDVLMLPPHARGRLFYSDDMRGFNFSRS
                     RSTIGEVSDKLVRYARFENRPSLVVQSALAAECLPGFAGENRLPLLDESVAPRIWIGS
                     AVTTPAHFDESSNIACVVSGTRRFTLFPPEQIANLYIGPLGHAPTGTPISLVSLADPD
                     FTRFPRFREALAASSSAELAPGDAIYIPPLWWHHVQSLARFNVLVNYWWKGSADTAAG
                     HDTALNCLLHCLLALRALPAQQRQAWKGIFDHYVFDAGEATSAHIPPYVRGVLGDISP
                     ELARQLKAFLVSQLQK"
     gene            3214..4842
                     /locus_tag="VFO53_14430"
     CDS             3214..4842
                     /locus_tag="VFO53_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HET8877521.1"
                     /translation="MSLTDERAPTATLFNHSYVFRAPDSTQRLPSAIRRIVIVGGGTA
                     GWMTAMILGHSLIPKGVEILLLESPSVGIIGVGEGSTPWLRGFFDSLGIEESEWMPAC
                     NATYKCGIRFDGWSTKPGFESYFHPFASMLDNLTMTQFVHNTHARLNGADVHAHPNRF
                     FIASQLAGHDLAPKPARNFPFDVWYGYHFDAGLLGEYLHRKAVERGVGYVPCHVTGAM
                     LDQHGDIGSVSIRDGEPVAADVFVDCTGFAGLLIQQALGTPFLPFASNLFNDAAVAMP
                     TPIGDDIPSQTVSAAMRHGWRWQIPLTNRFGNGYVYSSSFCSADDAERELRETLGLLD
                     ADAPARHLKMKVGRVTKHWNRNCLAVGLAQGFIEPLEATALLFIQRTATAFVECLEAG
                     DLGDGAHDRFNQRVNDHFEGTRDYIVTHYKTNTRRDTAYWRACAEMTSVSDSLRQLYG
                     LWMSGRGIAADVTRQTVGKGYPVFSWYCILAGMGIFPDANDLHAPSAAERRYSMEEID
                     NLLLRSAANFRPQRDVLKAIPKKVDERALQIYFW"
     gene            complement(4882..5709)
                     /locus_tag="VFO53_14435"
     CDS             complement(4882..5709)
                     /locus_tag="VFO53_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET8877522.1"
                     /translation="MHSAIRYALVACVVAIFACFSESAAAAGGKIKLQWFGQSATKIT
                     TVGGKVIVIDPWLTKNPKTPAEYKNLDAIGKMDIILVTHAHGDHLGDGPELAKKYNAP
                     LWGPAGLDQSLQTLGILPPNLAPRMGKGGTIQPFPGVKITQVHAEHSSEFLWHNPATD
                     KDETKVGGEPVGFIIELENGFRIYHMGDTSLFGDMKLIAEHYRPDLIMIPIGGHFVMN
                     PQDAAFATKTYFKPKHAIPMHYGTNPFLVGTPAQYIKAMGKTSTKIHDMKPGDTLEF"
     gene            5869..6207
                     /locus_tag="VFO53_14440"
     CDS             5869..6207
                     /locus_tag="VFO53_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET8877523.1"
                     /translation="MSLIGDTGAYSSTTLSRRDVEALAGSTVLDFGTSWCGVCRATAP
                     VVAQALAAHPGVRHLAIEDGPGRALGRSYRIKLWPTLVFLRAGREVERLVRPRDVRSI
                     VQALAKIDVA"
     gene            6364..6804
                     /locus_tag="VFO53_14445"
     CDS             6364..6804
                     /locus_tag="VFO53_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007940658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET8877524.1"
                     /translation="MRFMVIVKATPSSEAGNMPSEKLLAAMGAFNEELVKAGVMLAGE
                     GLQPSSKGVRVRIAGGTRTVIDGPFTETKELIAGFWLWQCKSKDEAIEWVKRCPDPMP
                     GEESVIEIRQIFEADDFGAELTPALRQQEARLRTQAAKLSSSAR"
     gene            6828..8090
                     /locus_tag="VFO53_14450"
     CDS             6828..8090
                     /locus_tag="VFO53_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012076533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET8877525.1"
                     /translation="MNGEPRSAAATIDAIYRTESRRIFATLIRLLGDFDVAEEALHDA
                     FRAALEHWPRDGVPANPRAWLVSAGRFKAIDGIRRRARFDALGDRIDEVEAIADESIA
                     FEREDIDDDRLRLIFTCCHPALSPDAQVALTLREICGLTTEAIAQAFLAAAPTLAQRI
                     VRAKGKIRDARIPYEVPAANELPARLDSVLRVIYLVFNEGYSASSGPALLRVDRSAEA
                     IRLGRLLVALLPDPEAVGLLALMLLQESRRAARVSPAGDVVLLDAQDRSLWNEAHIAE
                     GSGLVRRAMAAKRLGPYTMQAAIAAVHANAKRSELTDWPEIVALYGLLMRIEPSPVVE
                     LNRAVAVAMRDGPSAGLALVDAILARGDLGDYHLAHATRADLCRRLGYAQQARASYRR
                     AIALARQEPERRFLERRLAELAGTDAAA"
     gene            8166..8528
                     /locus_tag="VFO53_14455"
     CDS             8166..8528
                     /locus_tag="VFO53_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET8877526.1"
                     /translation="MMQYLLLCCFDEDRWDKLPATERDRIMQEYGKFQHDLAASGQYR
                     AGAKLGSIETATTLRERAGKPVITDGPFAETKEHLGGYHLIDCEDLDEAIAIARRIPT
                     LRAGGAVEVRPVESGHQA"
     gene            8540..8938
                     /locus_tag="VFO53_14460"
     CDS             8540..8938
                     /locus_tag="VFO53_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET8877527.1"
                     /translation="MKFLCLICAEKMLEQMPVTEAARHVGEYAAFTEGIRRSGHFIGC
                     NRLLPPATACTVRVRDGRVSTTDGPFAETKEQVGGYYVIEARDLNEAILVAARIPGAR
                     AGSVEVRPIADDVDARVALGAPGVAAQAAI"
     gene            8963..9376
                     /locus_tag="VFO53_14465"
     CDS             8963..9376
                     /locus_tag="VFO53_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8877528.1"
                     /translation="MKVEPYLFFDGRCEEAVEFYRKTLGAEVQMLMRYKDSPEPAQPG
                     MVPPGAENKIMHATLRIGDTTVMASDGHCTGKPKFDGFALSITAASEAEAERVFKSLG
                     DGGTVQMPLTKTFFSPRFGMLADRFGVPWMVIVAH"
     gene            complement(9440..11284)
                     /locus_tag="VFO53_14470"
     CDS             complement(9440..11284)
                     /locus_tag="VFO53_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018553341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET8877529.1"
                     /translation="MPLRIEDYALIGNTRTAALVGNDGSIDWMCVPRFDSNACFAALL
                     GDRGNGHWQIGPGHGVASVRRAYRPGTLILETEFETADGAVRVIDCMPVWNERTDIVR
                     IVEGVRGRVPLRVDLVMRFGYGAITPWVRRIDGALRATSGPDSLELRTPVALEGRNFT
                     TVAEFVVTEGQRVPFVMTWFASHEPRPVPIDAEAALEATERYWRTWVDHSMYDGRWAD
                     AVRSSLVVLKALTYAPTGGMVAAITTSLPEKIGGTRNWDYRYCWVRDATFTLYALLLA
                     GYHDEARAWREWLLRAAAGHPAELQILYGVGGERQLTEMVLPWLRGYENSAPVRVGNA
                     AAVQSQLDIYGELMDSLHLARSAGLDTDADTWKFERTLVNFLASNWQKPDSGIWEMRG
                     EPQQFTHSKVMAWVAMDRAVKAIENHGLDGPLERWRDVRSAIERDVCEHGVDKRRGVF
                     VQRYGSTELDASLLMIPLVGFLPPDDPRILKTIEAIERELVVDGLVLRYRTQTSVDGL
                     PPGEGFFLPCSFWLADCLALCGRHADAEALFERLLTLRNDVGLLSEEYDLANRRALGN
                     LPQALTHVALINTACNLSQRNGPSEHRSRASGENPPGGAVPAALPRAG"
     gene            11532..12479
                     /locus_tag="VFO53_14475"
     CDS             11532..12479
                     /locus_tag="VFO53_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HET8877530.1"
                     /translation="MMRNRIAEAVVFACVAAIAAPATAEQITFMTGPQGGSWIPLGGA
                     LKNMWEKAIPGLSIQETPGAGIANVRGVEENKAQVGFANSATTVDGIEGRAPYPGKTT
                     HVCQIANLYPQYFQVVALKDAHVKSFADMKGKSLVTQPKGNTGEILTGLVLKINGMSY
                     GSLAKTNYQAGYTDAVDMMKDGHAQVFTLGTTSPASAVMDLASARDIDLVPVDDKTMS
                     ELHRMNPGYVKLVIKAGTYPKQDRDVAAIAYSTHIVARCDMPEKIVYEMTKAMATHID
                     DMAAVVKPIHGLTARDMAVDIGVPFHKGAAKYYKEVGAM"
     gene            12486..>13534
                     /locus_tag="VFO53_14480"
     CDS             12486..>13534
                     /locus_tag="VFO53_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET8877531.1"
                     /translation="MADPASVPQDRPNALTTPPPGAASEASVERPLVSATPAVIVTDE
                     VPATAPTSRALRALVDGVAIAMSLYHMYVAAFGPPEAIIFRGTHLLFALTLVFLLYPT
                     RPGRPAWRAFDMALLALGFGFVLHIFVNYDYFINRIIYIDDLTASDAFFAIASIVIVL
                     EATRRVIGLALPLTAVAFLVYAVGFTHVRLPVLLEQLYLSTEGIFGSTLGVSASYVML
                     FVLFGAFMEKSGTGQLFMDFALSLTGHTAGGPGKVSVISSSLFGTVSGSAVANVMVDG
                     PITIPLMKKTGFRPPFAAAVEAVASTGGQLMPPIMGAAAFVMAEFLAVPYAQVALWAV
                     IPSLLYYIAVFFAVH"
BASE COUNT         2268 a         4536 c         4495 g         2235 t
ORIGIN      
        1 gacctgtcga attacgtcgg tttcggcaac cacgaggtgc agctgctcaa catccgcacg
       61 ggcacgttcg acacgtacac catttcctcg ccaacgaaca gcagcggcaa ggtgaagggc
      121 gtcgagcttg cctaccagca ggagctcgcc tacggcttcg gcgtgcaggc gaactacacg
      181 tacgccgacg cgcacgaaac cggcggacgc gatctcgtcg gtgcctcgcg caatacgtac
      241 aacgtcatcg gctactggga aggtcatggc ttcagcgccc ggctcgcgta cacgttccgc
      301 tcgcatttct tcgtcgggct cgaccgcagc acgcccgagt accaggacga cacgggcacg
      361 ctcgccgcat cattgtcgta cgcgatcaac gacaacgtcg cgctgacgtt cgacgcactg
      421 aacctcaaca acccgacgct caagtactac ggcgcgaaca ccgatcagcc gcgcgcgttc
      481 tacaagaacg gccggcagtt ctacgccggc gtacgcgtca agttctgagg cggcgacacg
      541 accggttccg ctcatcgacg cacacggcgg ggcagcgctt gccccgccgt ttttttggac
      601 aaccgccgga tgcacggaag tccccgaacc gctgccgcaa gtcgcgtcgc ggcggcgccg
      661 cgagcgatga cggcgctggc atgcgcgctg accatcttca ccgccgggtg cgtggcgccg
      721 cgcgcagggt cgggcgcggc cgcgtcctcg acgccatcgg ccgcgcccgc aagcgaggcg
      781 gcgcgcttcg tctacagtcc gtacaaatac gtgcctgtcg gatgggacgc cgatgccgcg
      841 gcgattgcga ccaccgccgg cggccgcgtc gtgccgctcg cggcggacgc ccgctcgacg
      901 cttcccgccg gtgttgtcgc ggtcacgctt gcgttcgcga ccggggaatg cggcgccgaa
      961 acatgggacg gtgtcgatcc cgacgtgctc gcgcgcgtca acgtgcggcg attcgagcgc
     1021 gcgggcatcg actacatcgt ctccacgggc ggtgaggccg gcacgttcag ttgcgcgacc
     1081 gacgccggca tggaacgctt cattgcgcgc tatgcctcgt cgcggctgat cggcctcgac
     1141 ttcgacatcg agcgcggaca atccgccgag ctcctcgcct cgctggtgcg ccgcatcaag
     1201 gcggcgaagg atcgtcatgc cgggcttcgc atcagcttca cccttgcgac ctgggcttcg
     1261 agcgacggca gcggcgcaag cctcaacgcc gacggcgaac gggtgatggc ggcgatccgc
     1321 ggcgccggtc tcgacgactt ctacgtcaac ctgatggcga tggactacgg aacggcgagc
     1381 gcgcggaatt gtgtcgtcgt cgacggtgtt tgcgacatgg ggcgctcggc gatgcaggcc
     1441 gcccgcaacg tggccgcgcg ctttgccgtg ccgctggcgc ggatcgaact gaccccgatg
     1501 atcggagtca atgacgtgat gaccaacgtg ttcacgctcg acgacgcggc cgcgctcggc
     1561 ggcttcgtgc gggaagcggg cctcggcggt ctgcacttct gggcactcga tcgcgacacc
     1621 ccgtgcccgc acggtgccgc gatcgtgctt tcggcctgca gcgggctcga tgcggcgccg
     1681 tatgcgttca cccgggcgtt cgcacgcgcg ctccaggcgc actgacgcgg ccggtcgcgc
     1741 gactttcaaa cggagacacc catgcgtgcg ttagcgttgt ccgtatcgat gatcctgctc
     1801 gtcgccgggt gcgccacgcc ggcgcaacgc gcggcacagg ttcagcgcga cgtcgacgaa
     1861 atggttgcgg tctacggccc gggatgcgaa cggctcggct acaaggccga ttccgacccg
     1921 tggcgcgagt gcgtgctgca ccttgcgacg aaggatcgcc tcgaacggcg cgacttcacc
     1981 acgacgaact gcatcggcag ccgcggcttc ttccagtgca gcacattctg agtcaggttc
     2041 cggccagcct catttctgca gctggctcac caggaacgcc ttgagctggc gcgcaagctc
     2101 gggcgaaatg tcgccgagca cgccgcgcac gtacggcgga atgtgcgccg aggtggcctc
     2161 gcctgcgtcg aacacgtagt ggtcgaagat gcctttccat gcctgcctct gctgtgcagg
     2221 gagcgcgcgc agcgcgagca ggcagtgcag caggcagttg agcgccgtgt catggcccgc
     2281 ggcggtgtcc gcgctgccct tccaccagta gttgaccagc acgttgaatc gcgcaagcga
     2341 ctggacgtgg tgccaccaca gcggcggaat gtagatcgca tcaccgggag cgagctcggc
     2401 cgacgatgac gccgcgagcg cttcgcggaa gcgcggaaag cgcgtgaaat cgggatcggc
     2461 gagcgaaacg aggctgatcg gcgtgccggt cggcgcgtgc ccgagcggcc cgatgtagag
     2521 attggcgatc tgctccggag gaaacagcgt gaagcgccgg gtgccgctca ccacgcacgc
     2581 gatgttgctc gattcgtcga aatgcgcggg cgtcgtgacg gcgctgccaa tccagatccg
     2641 cggcgccacc gactcgtcga ggagcggcag ccggttctcg cccgcaaagc cgggcaggca
     2701 ctcggcagcg agcgcactct gcacgacgag cgacggacgg ttctcgaagc gcgcatagcg
     2761 cacgagcttg tcgctcactt cgccgatcgt cgagcggctg cgcgaaaagt tgaacccgcg
     2821 catgtcgtcg ctgtagaaaa ggcgcccgcg cgcgtgcggc ggcagcatca gcacatcgac
     2881 ggcatgcgcg ctgccgaagc gcgccaggta atgcgcgacc gcttgcggcg actggcggcc
     2941 gctggccacc gccggccaat cggcaaccag cccgcgcagc accgcgggtc gataccgggg
     3001 cacgatctcg tcgcgaaaaa tcgcggcgtc gacgccgtgc cactcggcga tctgcgccgg
     3061 ttgcaccgcg gcgggcgcgt cggcgatggt gtcgttgttc acgcgcgtac tgtacgacaa
     3121 cgcggccgtt cgcgacgcgc gccttgattt gcgcaacgcc atgggtcacg cttcgcaccg
     3181 cggatcgaaa accgcgacca ccgaggcgaa cggatgagcc tgaccgacga gcgagcaccg
     3241 acggcaacgc ttttcaacca ttcgtacgtc ttccgcgcac cggacagcac gcaacggctc
     3301 ccgtccgcga tccgcaggat cgtgatcgtg ggcggcggaa cggcaggctg gatgacggcg
     3361 atgatcctcg gccattcgct gatcccgaag ggcgtcgaga tcctgctgct cgaatcgccg
     3421 agcgtcggca tcatcggcgt cggcgaaggc tccacgccct ggctgcgcgg cttcttcgac
     3481 agcctcggca tcgaggagtc ggaatggatg ccggcgtgca acgcaaccta caagtgcggc
     3541 atccgcttcg acggctggtc gacgaagccc ggcttcgaaa gctacttcca tccgttcgcg
     3601 tcgatgctcg acaacctgac gatgacgcaa ttcgtgcaca acacgcacgc gcggctgaac
     3661 ggcgcggacg tgcatgcgca tccgaatcgc ttcttcatcg cgtcgcagct cgccgggcac
     3721 gacctcgcgc cgaagcctgc gcgcaacttt ccgttcgacg tctggtatgg ctatcacttc
     3781 gacgccggtc ttctcggcga gtatctgcat cgcaaggccg tcgagcgcgg cgtcggatac
     3841 gtgccgtgcc atgtcaccgg cgccatgctc gaccagcacg gtgacatcgg gtcggtgtcg
     3901 atccgggacg gcgagccggt cgcggcggat gtcttcgtcg actgcaccgg cttcgccggt
     3961 ctcctgatcc agcaggcgct cggcaccccg ttcctgccgt tcgcgagcaa cctgttcaac
     4021 gacgcggcgg tcgcgatgcc gacgccgatc ggcgacgaca ttccgtcaca gacggtgtcg
     4081 gccgcgatga ggcacgggtg gcgctggcag ataccgctga cgaaccgctt cggcaacggc
     4141 tacgtctaca gttcgtcgtt ctgttcggcc gacgacgccg agcgcgagct cagggaaacg
     4201 ctcgggctgc tcgatgccga tgcgccggcg cgccatctca agatgaaggt cgggcgcgtt
     4261 acgaagcact ggaaccgcaa ctgcctggcg gtgggtctcg cgcagggctt catcgagccg
     4321 ctcgaggcga cggcgctcct gttcatccag cgcacggcga ccgcgttcgt cgagtgcctc
     4381 gaagcgggcg atctcggcga tggcgcgcac gaccgcttca accagcgcgt caacgaccat
     4441 ttcgagggca cgcgtgatta catcgtcacc cactacaaga cgaacacgcg ccgcgacact
     4501 gcctactggc gtgcctgtgc ggaaatgacg agcgtctccg attcgctgcg ccagctgtac
     4561 ggcctgtgga tgtcgggacg cggcatcgcg gccgacgtca cccggcagac ggtcggcaag
     4621 gggtatcccg tgttctcgtg gtactgcatc ctggccggga tgggcatttt ccccgacgca
     4681 aacgacctgc atgcgccgag cgccgccgag cggcgctaca gcatggaaga gatcgacaac
     4741 ctgcttttgc gcagcgccgc gaacttcagg ccgcagcgcg acgtcctgaa ggccatcccg
     4801 aagaaggtcg acgagcgcgc gctgcagatc tacttctggt gatgcggccg ccggtgcgcc
     4861 cgtgcaggcg tcgcgcccgc gtcagaattc gagcgtatcg cccggcttca tgtcgtggat
     4921 cttcgtcgat gtcttcccca tcgccttgat gtattgggcc ggcgtgccga cgaggaacgg
     4981 gttcgttccg tagtgcatcg gtatcgcgtg cttcggcttg aagtatgtct tcgtcgcgaa
     5041 cgccgcgtcc tgcggattca tcacgaaatg gccgccgatc ggaatcatga tgaggtcggg
     5101 acggtaatgc tcggcgatga gcttcatgtc gccgaagagg ctcgtgtcgc ccatgtggta
     5161 gatgcgaaag ccgttctcga gctcgatgat gaagccgacc ggctcgccgc cgaccttcgt
     5221 ctcatccttg tcggtggccg ggttgtgcca gaggaactcc gagctgtgct cggcgtgcac
     5281 ctgcgtgatc ttcacgccgg gaaacggctg gatcgtgccg cccttgccca tgcgcggcgc
     5341 aagattcggc ggcaggatgc caagcgtctg cagcgactgg tcgaggccgg cgggtcccca
     5401 gagcggtgcg ttgtacttct tcgcgagctc gggaccgtcg cccagatgat cgccgtgcgc
     5461 gtgcgtcacg aggatgatgt ccattttgcc gatggcgtcg aggttcttgt actcggccgg
     5521 tgttttcgga ttcttggtga gccaggggtc gatgacgatt accttgccac cgaccgtcgt
     5581 gatcttcgtc gcggactgcc cgaaccactg cagcttgatc ttgccgcccg cggcagccgc
     5641 gctttccgag aagcacgcga agatcgcgac gacgcacgcg acgagcgcgt aacgaattgc
     5701 cgaatgcatg aatttcctcc ttcagcggtt tttggtaggc acccgttcga gcgcgaagca
     5761 tagaagcttc ccctcgccca ttcaacaggc gacgaaaact ggaagtcgcg agcgaacggg
     5821 gttaccggcg cgaggtctca ggaagccaac gagtccgcgc gacacgcaat gtcactgatt
     5881 ggcgacaccg gcgcctattc gtcgaccacg ctttcacgcc gggacgtgga agcgctggcg
     5941 ggctccaccg tcctcgattt cggcacgtcg tggtgcggtg tctgccgcgc caccgcaccg
     6001 gtcgtcgcgc aggcgctggc cgcgcatccc ggcgtgcgcc acctggcgat cgaggatggc
     6061 ccggggcggg cactcggccg ttcgtatcgg atcaagctat ggccgacact cgtcttcctg
     6121 cgcgccggcc gcgaggtcga gcgcctcgtc cggccgcgcg acgtgcgatc gatcgtgcag
     6181 gcgctcgcga agatcgacgt cgcgtgattt ccattcgccg gcctcgccgg tcccggaaca
     6241 ttaaaattcc cgcgacattg tcgatttccg tgcgtgcggt tcgacttgac gtagtgcgcg
     6301 tcaagcgcac ccgaggcagc cggcgggcgc cggccgcgcg attcgagatc aggagagacc
     6361 acgatgcgat tcatggtgat agtcaaggca acgccgagca gcgaagcagg caacatgccc
     6421 agcgagaagc tgctggccgc gatgggcgcg ttcaacgagg agctcgtcaa ggcgggcgtc
     6481 atgctggccg gcgagggttt gcagccgagc tcgaagggcg tgcgcgtcag gattgcaggc
     6541 ggcacacgca cggtcatcga cggcccgttc accgaaacca aggagctgat cgccggtttc
     6601 tggctgtggc agtgcaagtc gaaggacgaa gcgatcgaat gggtcaagcg ctgccccgat
     6661 cccatgcccg gcgaggaatc ggtgatcgag attcgccaga tcttcgaggc cgacgatttc
     6721 ggtgccgagc tcacgcccgc gctgcgccag caggaagcgc gtttgcgcac gcaggcggcg
     6781 aagctgtcgt cgtccgcgcg ctgacgcgac agcatgcgtt cgctgcgatg aacggcgagc
     6841 ctcgaagcgc cgccgcgacg atcgacgcga tttaccgaac cgaatcgcgg cgcatcttcg
     6901 cgacgctgat ccgcctgctc ggcgatttcg atgtcgccga ggaggccttg cacgacgcat
     6961 tccgggcggc gctcgagcat tggccgcgcg acggcgtgcc cgccaatccg cgcgcatggc
     7021 tcgtgtcggc ggggcgcttc aaggcaatcg acggcattcg ccggcgcgcg cgattcgacg
     7081 cgctcggcga ccggatagac gaggtcgaag cgatcgccga tgaatcgatc gcgttcgaac
     7141 gggaagacat cgacgacgac cggctgcggc tcattttcac ctgctgccac ccggcgttgt
     7201 cgcccgatgc gcaggtcgcg ctcacgctgc gcgagatctg cgggctcacc accgaagcga
     7261 tcgcgcaggc gtttctcgcc gccgcgccca cgctcgcgca gcgcatcgtg cgcgcaaagg
     7321 ggaagatccg cgatgcgcgc atcccgtacg aggtgccggc ggcaaacgag ctgcctgcgc
     7381 ggctcgacag cgtgctgcgg gtgatctatc tcgtcttcaa cgaaggctac tcggcgtcgt
     7441 ccggcccggc gctcctgcgc gtcgaccggt ccgccgaagc gatccgcctc gggcggctgc
     7501 tcgtcgcgct gctgcccgac cccgaggccg tcggattgct cgcgctgatg ctgctgcagg
     7561 aatcgcggcg tgcggcacgc gtgtcaccgg ccggcgacgt cgtcctgctc gatgcgcagg
     7621 atcgctcgct gtggaacgag gcgcacatcg ccgaaggatc cgggctggta cgacgggcca
     7681 tggcggcgaa aagattgggc ccgtacacga tgcaggcagc gatcgcggcc gtgcacgcca
     7741 acgcgaagcg cagcgagctt accgactggc cggagatcgt cgcgctctac gggctgctga
     7801 tgcggatcga gccgtccccc gtcgtcgagt tgaatcgtgc cgtcgcggtc gccatgcgcg
     7861 atggcccgtc cgccgggctc gcgctggtgg atgcgatcct tgcgcgcggc gaccttggcg
     7921 actatcacct cgcgcacgcg acccgcgccg atctctgccg gcgtcttggc tatgcacaac
     7981 aggcgcgtgc gtcgtatcgg cgcgcgatcg cgctcgcaag gcaggagccc gagcgccgct
     8041 ttctcgaacg gcgccttgcc gaactggccg gcacggacgc cgcggcgtga gcgccgcacg
     8101 cactgctgtc gattccgcac gcgcgcattc gactacgcac cgttcccatc cgaaacaagg
     8161 agacgatgat gcaatacctg ctgttgtgct gcttcgatga agatcgctgg gacaagctcc
     8221 ccgcgacgga acgcgaccgg atcatgcagg agtacggcaa gttccagcac gaccttgccg
     8281 cgagcggaca atatcgtgcc ggcgcgaagc tcggatcgat cgagaccgcg acgacgctgc
     8341 gcgagcgggc cggcaagccg gtcatcaccg acggtccgtt cgccgaaacg aaggagcacc
     8401 tcggcggcta ccacctgatc gactgcgagg atctcgacga agcgatcgcg atcgcgcggc
     8461 gcattcccac gctgcgcgca ggcggtgccg tcgaagtgcg tccggtcgaa tccgggcacc
     8521 aggcataaag gacgtcacga tgaaattcct gtgccttatc tgtgccgaga agatgctcga
     8581 gcaaatgccg gtcaccgaag ccgcgaggca cgtgggcgag tacgccgcat tcaccgaagg
     8641 aattcgcaga agcggacact tcatcggctg caatcgcctg cttccgcccg ccacggcgtg
     8701 caccgttcgc gtgcgcgacg gcagggtctc gacgaccgac gggccgttcg cggaaacgaa
     8761 ggaacaggtc ggcggctact atgtcatcga agcgcgcgac ctcaacgaag cgatcctcgt
     8821 cgccgcgcga attcccggcg cgcgcgccgg gtcggtcgaa gtacgcccca tcgccgacga
     8881 tgtcgacgcg cgcgttgcgc tcggcgcgcc gggcgtcgcc gcacaggcgg cgatttaacc
     8941 ctcgttcctg aaaggagacg acatgaaggt cgagccctac ctgttcttcg acggtcgctg
     9001 cgaggaagca gtcgagttct accggaagac actcggcgcc gaagtgcaga tgctgatgcg
     9061 ctacaaggac agccccgagc ccgcgcagcc cgggatggtg cctcccggcg ccgaaaacaa
     9121 gatcatgcac gcgacgctgc gcatcggcga cacgacggtg atggcttccg atggccactg
     9181 cacgggcaag ccgaaattcg acggcttcgc gctttcgatc accgctgcca gcgaagctga
     9241 agccgaacgc gttttcaagt cactcggcga cggcggaacg gtgcagatgc cgctcacgaa
     9301 gacgttcttt tcgccgcgct tcggcatgct tgccgatcgc ttcggcgtgc cgtggatggt
     9361 gatcgtcgcg cactagactc gcgccgcggc cggcggccgc aggtggcccg tcacggagga
     9421 atcggcagtg cctggcttgc tagccggccc tgggcagcgc ggcgggcacg gcgccgccgg
     9481 gcggattctc gccgctcgcg cggctgcgat gctccgacgg gccattgcgt tgcgacagat
     9541 tgcatgcggt attgatcagc gcgacgtgcg tcagcgcctg cggcagattg ccgagcgcgc
     9601 gccggttcgc aaggtcgtac tcctcggaca gcaatccgac atcgttgcgc agcgtgagca
     9661 ggcgctcgaa caacgcctcg gcgtcggcat ggcggccgca caaggcaagg cagtccgcaa
     9721 gccagaacga gcacggcagg aagaagcctt cgccgggcgg caggccgtcg acgctcgtct
     9781 gcgtccggta gcgcagcacg aggccgtcga cgacgagctc gcgttcgatg gcctcgatcg
     9841 tcttcaggat ccgcgggtcg tccggtggca ggaagccaac cagcggaatc atgagcaggc
     9901 tcgcgtcgag ctcggtggac ccgtagcgct gcacgaatac gccacggcgc ttgtccacgc
     9961 cgtgctcgca cacgtcgcgc tcgatggccg agcgcacgtc gcgccagcgc tcgagcggtc
    10021 cgtcgagacc gtggttctcg atcgccttca ccgcccggtc catcgccacc cacgccatca
    10081 ccttcgaatg cgtgaattgc tgcggctcgc cacgcatctc ccagatgccg ctgtccggct
    10141 tctgccagtt cgacgcgagg aaattgacga gcgtgcgctc gaatttccac gtgtcggcgt
    10201 cggtgtcgag gccggcgctc cgcgcgaggt gcagcgagtc catcaattcg ccgtagatgt
    10261 ccagttgcga ctgcacggcc gcggcgttgc ccacgcgcac cggcgccgag ttttcgtagc
    10321 cgcgtagcca cggcagcacc atttcggtca actggcgctc accgccgacg ccgtacagga
    10381 tctgcaattc ggcagggtgg ccggcggccg cgcgcaacag ccactcgcgc cacgcgcgcg
    10441 cctcgtcgtg atagcccgcg agcagcagcg catagagcgt gaacgtcgca tcgcgcaccc
    10501 agcagtagcg gtagtcccag ttgcgcgtgc cgccgatttt ctccggcagc gacgtcgtga
    10561 tcgccgccac catgcctccg gtcggcgcat aggtgagtgc cttcagcacg acgagcgacg
    10621 agcgcaccgc atcggcccag cgcccgtcgt acatcgagtg gtcgacccaa gtgcgccagt
    10681 atctttccgt ggcttcgagc gccgcctcgg cgtcgatggg gaccgggcgc ggctcgtgcg
    10741 atgcaaacca tgtcatcacg aacggcacgc gctggccttc cgtcacgacg aactcggcga
    10801 cggtcgtgaa gttgcggccc tcgagcgcga ccggcgtgcg cagctcgagc gaatcggggc
    10861 cggaggtcgc gcggagcgcc ccatcgatgc ggcgcaccca cggcgtgatc gcgccgtagc
    10921 cgaagcgcat gacgaggtcc acgcgcagcg gcacgcggcc gcgcacgccc tcgacgatgc
    10981 gcacgatgtc ggtgcgctcg ttccacaccg gcatgcagtc gatgacgcgc acggcgccgt
    11041 cggccgtttc gaactcggtc tcgaggatca gcgtgcctgg acggtacgcc cgacggaccg
    11101 acgcaacgcc gtgccccggg ccgatctgcc agtgcccgtt gccgcgatcg ccgagcagcg
    11161 ccgcgaagca ggcgttggag tcgaagcgcg gcacgcacat ccagtcgatc gagccgtcgt
    11221 tgcccacgag cgctgctgtg cgggtgttgc cgatcagtgc gtagtcttcg atgcgcagcg
    11281 gcacggcgac gcttcctttg tcgtggcgcg cgcatgcgca ccatcgatgc ttgccgcgcc
    11341 gcacgacggc tcgatcgcga cgcgattaaa tttccgactc gccgaaatta tcatacgcgg
    11401 ccgagacacc ggcccagggc cggtccgatg cgatcgcttg accgccttgc gacgcccgca
    11461 cagaatgcgt tcaccgcgga tgagacccgg cgcattcccg gcggccgggc gaccgcacag
    11521 gaggagtcga catgatgcgc aatcgtatcg ccgaggccgt tgtgttcgcc tgcgtcgcgg
    11581 ccatcgccgc gccggcaacc gccgagcaga tcacgttcat gacgggaccg cagggcggat
    11641 cgtggatccc gctcggtggc gcactgaaga acatgtggga aaaggcgatc ccgggactgt
    11701 ccatccagga gacacccggc gccggcatcg ccaacgtgcg cggcgtcgag gagaacaagg
    11761 cgcaggtcgg gttcgccaat tcggcgacca ccgtcgacgg catcgagggc cgcgcgcctt
    11821 atccgggaaa gacgacccac gtctgccaga tcgcgaatct ctatccgcag tacttccagg
    11881 tcgtcgcgct caaggacgcg cacgtgaagt cgttcgctga catgaagggc aagtcgctcg
    11941 tcacgcagcc gaagggcaac accggcgaga tcctcacggg gctcgtgctg aagatcaacg
    12001 gcatgagcta cggctcgctc gccaagacca actaccaggc cggctacacc gacgcggtcg
    12061 acatgatgaa ggacggccac gcgcaggtat tcacgctcgg cacgacgtcg cccgcgtcgg
    12121 cggtgatgga cctcgcgagc gcacgcgaca tcgatctcgt gccggtcgac gacaagacga
    12181 tgagcgagct gcacaggatg aatcccggct acgtcaagct cgtcatcaag gccggcacgt
    12241 atccgaagca ggaccgcgac gttgccgcga tcgcctactc gacgcacatc gtcgcgcgct
    12301 gcgacatgcc ggaaaagatc gtgtacgaga tgacgaaggc gatggccacg cacatcgacg
    12361 acatggccgc cgtggtcaag cccatccacg ggctgacggc cagggacatg gccgtcgaca
    12421 tcggcgtccc gttccacaag ggcgccgcga agtactacaa ggaagtcggc gcgatgtgat
    12481 cgcgcatggc tgatcccgcg tcggtgccgc aggaccgtcc caacgcactg acgacgccgc
    12541 ctccgggggc agcgagcgaa gcgagcgtgg agaggcctct cgtttccgcg actccggccg
    12601 tcatcgtcac cgacgaagtc ccggcgaccg cgccaacgag ccgggcgctg cgcgcgctcg
    12661 tcgacggcgt cgcgatcgcg atgtcgctct accacatgta cgttgccgcc ttcggtccgc
    12721 cggaggcgat catctttcgc ggcacgcacc tgctgttcgc gctgacgctc gtcttcctgc
    12781 tgtatccgac gcgccccggc cggcccgcgt ggcgggcgtt cgacatggcg ctgctggcgc
    12841 tcggcttcgg attcgtgctg cacatcttcg tcaactacga ctacttcatc aatcgcatca
    12901 tctacatcga cgatctcacg gcatcggacg cgttcttcgc catcgcgtcg atcgtcatcg
    12961 tcctcgaagc gacgcggcgc gtcatcgggc tcgcgctgcc gctcacggcc gtcgcgttcc
    13021 tcgtctacgc ggtcggcttc acgcacgtcc gcttgcctgt gctgctcgag cagctctacc
    13081 tgtcgaccga aggcatcttc ggatcgacgc tcggcgtttc ggcgagctac gtgatgctgt
    13141 tcgtgctgtt cggcgcgttc atggaaaaaa gcggcaccgg acagctgttc atggatttcg
    13201 cgctgtcgct gaccggtcat acggccggcg gcccggggaa ggtttcggtc atttcgtcga
    13261 gcctcttcgg cacggtgtcg gggagcgccg tcgccaacgt gatggtggat gggccgatca
    13321 cgattccgct gatgaagaaa accggcttcc gcccgccgtt cgccgccgcg gtcgaggcgg
    13381 tggcgtcgac gggcgggcag ctgatgccgc cgatcatggg cgcggcggca ttcgtgatgg
    13441 cggagttcct cgcggtgccg tatgcgcagg ttgcgctgtg ggcggtgatt ccgtcgctcc
    13501 tctactacat cgcggtcttc ttcgccgtgc actt
//
LOCUS       DASSVX010000337        18528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1598525, whole genome shotgun sequence.
ACCESSION   DASSVX010000337 DASSVX010000000
VERSION     DASSVX010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 18528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18528
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598525"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VFO53_14485"
     CDS             complement(<1..1252)
                     /locus_tag="VFO53_14485"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET8877532.1"
                     /translation="MSPFALKVFPFLAWRHRVNRVTLRADVLAGLVGAVMVLPQGVAY
                     ATLAGMPPEYGLYGAMLPAIVGALWGSSWHLMSGPTNATSLMVFATVSALAAPFTPAY
                     VAIVLTLNLMVGIVKLGLGVARLGSLVNFISTSVVIGFTAGAGLLIIGAQLRNFFGID
                     VPQQASFIAGLRDFVTHLGATDPAVLGVGVFTLVAAIVGRRLLPRVPYMLTGIVAGAL
                     CAWVLSLTGVANVPTIGALPSAVPPLSVPEMSLQTWQTLAPIALALTVIGLSEAISSA
                     RAVALKSGQRIDGNQEFIGQGLANIVGAFTSSYPTSGSFNRTGANYEAGARTPLACVF
                     SAVLLLVILLLVRPLAGYLPVASMAAVLFIVAMGLIDVQAMRRVWRTSRADALTLAVT
                     FIATLTIRLEVAILVGVLVSLLIYL"
     gene            complement(1330..2130)
                     /locus_tag="VFO53_14490"
     CDS             complement(1330..2130)
                     /locus_tag="VFO53_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8877533.1"
                     /translation="MRTIKGYSLPGEGPSTLISVVTVVGLLAFWWVATHFGWIKDLFL
                     PTPEKIVTSFGEAWRGDIQGGKPLAEHFWWSLYRVFAAFVLAVLTAVPVGVAMGVSRI
                     ARGIFDPPIEFYRPLPPLAYLPLIVIWFGIDETAKVVLIYLACFAPLAMAARAGVRSV
                     TIEQINAAYSMGGSKWQVIFHVVIPAAMPEILTGMRIAIGFGWTTLVAAEMVAATVGL
                     GQMVLNASNFLRTDVVIMGIIVIGVVAYLFDLLMRYVEHLVVPWKGKV"
     gene            complement(2330..3127)
                     /locus_tag="VFO53_14495"
     CDS             complement(2330..3127)
                     /locus_tag="VFO53_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8877534.1"
                     /translation="MSKLEIRNVSVKYAGSSGRTTLALSNVNLGLDPGDFVVALGASG
                     CGKTTLLSCIAGFMQPSAGEIVLDGRPVIGPGAERGVVFQKHALMPWLNVVENVEFGL
                     RMRGVPKGERRAVALDKLRLVGLEKFVTYPVYQISGGMQQRVGLARALASDPEVMLMD
                     EPLGALDALTREQQQELILYLWQTTRKMFFFITHSVEEALFLATELIVMTPSPGRIAH
                     RYTLDFGRKFVECRDARKVKADPRFIAVREEVLQLIHSAPADAEAAA"
     gene            complement(3260..4504)
                     /locus_tag="VFO53_14500"
     CDS             complement(3260..4504)
                     /locus_tag="VFO53_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877535.1"
                     /translation="MANHRMSAFNLLAAISATCALVAVPQSSSAVGVLIYAPEAQERC
                     WIERIQYTDNWYNATGHCDETNGRGDAYPVHVPFSAVGRYDASTNEASETIRIVKGGL
                     EIPTHDYYGTITSTLSCGADPWQKATIDCGVTSVSATETIPQYFVDSLVRKRRRPFTS
                     DMTDAQRAAVTQQYLASIHRLHERVEEHPGTGVVLDSGRANAAPSQTMSAGGTALAPS
                     PRSKEVGALSALRAGGTTIRTPEPATGASHSAPATGAAMRTIAILEPAPGSSVRQGQI
                     RVRIRDAALGAETSNGVATVELSALAPRAASRAQAASGTAKVASWQVPLAQLTQGAVV
                     PRSISGTFAGPTLLRVRTSPTGAWSDGVSFDITSDAQTQARTPTPPDWSAAPPAGAPA
                     QPGNVPGTGASAFRAPASSFGH"
     gene            complement(4549..6297)
                     /locus_tag="VFO53_14505"
     CDS             complement(4549..6297)
                     /locus_tag="VFO53_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877536.1"
                     /translation="MNRRSRMTCTALVAFGASAVVVLAHAQFPASGLRQGAGVDTGSP
                     ASSLHGGGAPSAPASAGAMPAWTPRAAAANGASAVLPAHDLQQANLPADLAHLDDNAI
                     IIVGGKQVNAADVKRGVLALDDHAIIIVGGRQTPAATLKRELGTTKPPSVKGMAAATN
                     AGVDPPATRPSTGATTAGVTAAGATSAGATQGAKRPARVSLVANEHLSIVAKKRANLQ
                     REYAQLREGQDAVAAGFRPPSQNPTVAASATAATNFPANPAALRTSVGTRPNAVGDPG
                     IVSVNGRIQDIRVMPDGKLVIAGNALGDRPGGARLTGGALASNPVPLLVAKWNAGAIE
                     ANIPGGTRGVPDEPKAVLEVKTASGATYRFDGITFVAARAEATVKDADRIAQYLHVDA
                     GPTWPQASIAKNAVARWIDGESIDCPRTGTDTLRFRNVNGFEVVGASMVHARTDSGDR
                     DMAGNAGGRVYSPGYAFGEWRDEVVSRYTGDGSIGAPATFVSALPVSWGIFRSHTSPG
                     FSLDVEFFDPLEFPMITAFGTAVFNLGKIPGTSVPVPFTTPPKPVIHTSGPHDICLSG
                     YAITSLTVYGPVGITP"
     gene            complement(6294..6581)
                     /locus_tag="VFO53_14510"
     CDS             complement(6294..6581)
                     /locus_tag="VFO53_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877537.1"
                     /translation="MQASLVTVQGSKTWQVSFGQLAAGFVVPADKICSCTNGRATLKV
                     RPSGTSTWQDIVAFNVVAATPGSATTSLPSAGFGVRTSNDEPLRSRKETLR"
     gene            complement(6670..7359)
                     /locus_tag="VFO53_14515"
     CDS             complement(6670..7359)
                     /locus_tag="VFO53_14515"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HET8877538.1"
                     /translation="MLVAVALAASMPALAADARAVGAAPPATAADRASRPVVTIYEFR
                     SGVSEIGARGATDMFVTALVKSGRFRVVERSRLNEGVVREKQLNANGLATGDVGSTQL
                     QGARFVFEGSVSQANASEDQRASGIGVGGMEVGGGSNRDTLAIDVRVVDASSGEVLDV
                     VTAQGSVTATSSSLSGVGNLLSAVAGSRGYGIPVVPDVHVQQQHRESVDEALRSAIEA
                     AVATLGERFGH"
     gene            complement(7438..7983)
                     /locus_tag="VFO53_14520"
     CDS             complement(7438..7983)
                     /locus_tag="VFO53_14520"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HET8877539.1"
                     /translation="MTFGKLLSSLVLLSIACAADAQGLKQRKPGLWELQYTAEGPAMQ
                     AEQERFRNELASMPPEKRARVEALMKQRGTGPGGAPVGQMRFCLTPEDLAEQSSSGYL
                     KGLNDESNCKSKILSESVSEVRIHATCRNPKGGTREVDTRVYDISADHYAVDMTSRGT
                     GNDMHMQQKARWIGGDCKGAF"
     gene            complement(8045..8266)
                     /locus_tag="VFO53_14525"
     CDS             complement(8045..8266)
                     /locus_tag="VFO53_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877540.1"
                     /translation="MSARMDTFIVRIYRKVARGAEEPAGTVEHVESGDRVAFSDARQL
                     LTRLLTSRRHRGAAVQGAADGNDEQPTRD"
     gene            complement(8381..11512)
                     /locus_tag="VFO53_14530"
     CDS             complement(8381..11512)
                     /locus_tag="VFO53_14530"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET8877541.1"
                     /translation="MPSSPRLAKLSAPRTGTALVRARLHRLLDEAVERGAAFIAAGPG
                     TGKSTLAVTWATPRAGRLLWFRADAADADPAEAFGYFRQLAGTSKGAQGLPSYRPRDI
                     DRLDLFATAFFRTFFRVVPAASTLVVDDAHAASGDAFLTLLAAAIREAPPDVAVVVLS
                     RREPAGPLLDDVASGRVHVLDTAALAFDASEAVSLLAHRMDAATARRLHARTDGWAAG
                     MLLLAQSGESAPARHAASARIAAYFDERVLAVFDDSELRTLAVVSLLPEVDAASLARL
                     GLDGGAGQLLERLRRENAFVVRLERGPASWRLHDLLRDALRARFRTIADAAWRRTALL
                     AAAPLAAAAGHVRDAVQMYLDANEGSRAKGIAEEHARSLVRAHRLAELDAVVAALSPE
                     EAHGSRVLQIALGESAWQRNHASSAVACFERAYAMIDDETPSADGLLLASSALNAIFE
                     GWQSYDGTRTWADRLRRHLGARERITDPHDVLRIDRTWLQTADQLWDDSLCDRPALFD
                     RVLAQVRDPPPGVSVDEIVATSAVLVESSGYNLHDEARFQATITATAAWLQHPDLAPL
                     VKAMWLNSYAGLGKHWPSPGVRLPAAGGNPSLELAYDLARSHGGDALAFIAADFLCHG
                     AIGDNDLPRARHWLERQRESANPRHVVQTVTLLATEAAVLGLAGEWRRSIATFERADA
                     LAREHAHPEDDYWNRSLSRYRVLIASGEAARAREGLLRDAPGYPEGLRRDFAVVLADV
                     ALADIEWKAHGAIPAPLVAKIMERTRAYNWRGIAMLLRPVVARICSEALRLGIEPDFA
                     RRLIRDKRLPAPSPYDANWPWPVRIHALGGLRIVVEGQPLVFGARAQRKPLDLVKAIV
                     AHGPAPVDAALVLDALWPDAEGGAARASLDMTLMRLRKLLGRDDALTLDAGRIGFNPA
                     FVWIDAYAFAFGENEHYAGPLFGHEAVAPWWASARERLHQRFLRRTVERGRELESERR
                     FADALAVYEAALAEDSLAEELYQGTIRCHIAQGRHADAMRAFRRCREQLSIVLSVRPS
                     ADTLALVATLGAR"
     gene            complement(11602..12600)
                     /gene="tauA"
                     /locus_tag="VFO53_14535"
     CDS             complement(11602..12600)
                     /gene="tauA"
                     /locus_tag="VFO53_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_function: GO:0030977 - taurine binding [Evidence IEA];
                     GO_process: GO:0015734 - taurine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HET8877542.1"
                     /translation="MRKVWFIGLALAFGAGIASAQQKEVTIAYQDMLDPYRVSQDAKD
                     LEKATGYKINWKQFGGGGEVIKAMASGAVQIGEVGSAGIAAAVSRGEPFELFWILDDI
                     GDAEALVAKNGSGINSVKDLKGKKIAMPFNSTTHFHTMVALEQAGINPKDVQILNMRP
                     PEVRAAWQRGDIQATWIWDPVLSEVKKDGKVILTSGKLSAETGKATFDGYVVNKDWAK
                     QHHDFMVKFVKVLAAADEQYRTNKAKWTPDSPEVKAVAKWSGAKPESVPEGMALYRFP
                     TLAEQNSKWLGGGKSSVAAKALEATSKFELSQKQIEKTLPDYSTVVTDQYVKDAMK"
     gene            complement(12633..13976)
                     /locus_tag="VFO53_14540"
     CDS             complement(12633..13976)
                     /locus_tag="VFO53_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET8877543.1"
                     /translation="MGHAEFTLANQPAQKPLSLDELWMPFTPNRDFKSDPRIVVRAEG
                     MYYYNDRGEKIIDASSGLFCVAAGHCRKEISEAVHRQLGELDFVAPFLRGHPKSFELA
                     SRIAELTPGDLNRIFFVNSGSEAVDTAMKAALAYHQARGQTGRTMFVSRERAYHGVNF
                     GGVALSGIVNNRRKYGPGIAGVAHMRHTQLEQNRYTPGEGAHGAELADDLLRFVNLYG
                     AENIAACFVEPIAGSTGCLVPPKGYLARLREICDTHGILLVFDEVITGFGRTGQPFAA
                     QSFGVTPDIMTIAKALTNAAQPMGAVAISERIHDTVMAAAQDGAIEFFHGYTYSGHPA
                     ACAAGLATLDIYRNEGLFERGRELAPYFQDAMFSLKGTKNVTDVRGYGMFAALDVESD
                     GAPGRRGNVLQKKLFDNGLNLKNTGDCALVAPPLISERQHVDTIVDILRKTVSAL"
     gene            14240..15727
                     /locus_tag="VFO53_14545"
     CDS             14240..15727
                     /locus_tag="VFO53_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET8877544.1"
                     /translation="MWNQLIQLSFRSKVGLQAQIREMLVAAILDGHIPVGVALPSTRV
                     LAAQLGVARNTVALAYELLVNEGYLLTKSRSGHYVNPEIVAGRVALPRAPGVGDMPAQ
                     VDWAPRLRGGVLMQRNIVKPRDWQRYPYPFIYGQFDPGLLPIPGWRECSLQATSVSEV
                     RGWAGDLIDADDALLIEQIQTRLLVRRGVFAAPDEILVTVGAQHALFLLATLLVDAAT
                     TVGIENPGYPDARNIFASRTASIVPLPLDDAGLALGPALESCEYVYLTPSHQCPTNVT
                     MPLARREALLAWAHANDRILIEDDYEIELGLEGHAHPALTSLDRSGHVVYVSSLSKTL
                     APGIRSGFIVGRRELIRELRALRRLMLRHPAANNERSVGLFLAMGHHDALLRRLRQAY
                     AERSRVMAAALATHLPGVRFSTVSGASSFWLRFPTHVDTRVLADAAALKGVLIEPGDV
                     FFSGGGEPAPRNYARLGFASIDAARIDAGIATLAGACAEVAAASA"
     gene            15814..16929
                     /gene="ald"
                     /locus_tag="VFO53_14550"
     CDS             15814..16929
                     /gene="ald"
                     /locus_tag="VFO53_14550"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET8877545.1"
                     /translation="MRIGVPKEIKVLENRVGLTPGSVRELVHRGHELVMQDGAGQGIG
                     VANADYAREGATIAQSAADVFSSCDMIVKVKEPQAAERAMLRPGQILFTYLHLAPDPA
                     QTRDLVASNAVCIAYETVTSPAGGLPLLAPMSEVAGRLAVQAGAHYLEKPHGGKGILL
                     GGVPGVAPASVVVIGGGVVGTHAIEIALGMGARVTVLDRSVDALKRLWTQYGAALQTL
                     FSTVDAIERQVAAADLVVGGVLVAGAAAPKLVTRAMVAKMQPGSVVVDVAIDQGGCFE
                     TSHPTTHAEPTYVVDGVVHYCVANMPGGVPRTSTFALNNATLPFVVAIADKGWKRALA
                     EDAHLQQGLNVALGRVTCRPVADALGYAWTDAASFLA"
     gene            complement(17110..18195)
                     /locus_tag="VFO53_14555"
     CDS             complement(17110..18195)
                     /locus_tag="VFO53_14555"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8877546.1"
                     /translation="MASSNRRIGIIGIGFGMQAYVPGFASEGWEVAALCSRHADRTRK
                     AADEAGIRDIHTDPLELIGRDDLSAIGIATPPGTHCALAIAALDAGKHVMCEKPFALD
                     AGQAIAMTRAAARSGKTAMVGHEFRHAPQRRYIKQLLDDGYIGRFQLCTVELFLDRYV
                     TREPRALTWQAFRSEGGGVLGALGSHYIDGLRDWFGDVVSASGRLMTLRPDLVDPATG
                     RELQGETDDAFVFTLAFANGGMATMTCSFAATPARGARIAVMGDRGTLIAEQAGPNPP
                     EDGRVIASRDGEPLRPLETPSQFTPFTDGRDPRLMAFRLLVRDFTRGIDAGTSPSPNF
                     TDGLRCQQVLDAIRESDATGRTIAIAC"
BASE COUNT         2947 a         6236 c         6353 g         2992 t
ORIGIN      
        1 tgaggtagat caacagcgac acgagcacgc cgacgagaat cgccacttcg aggcggatcg
       61 tcagcgtggc gatgaacgtc accgccagcg tcagcgcgtc ggcgcggctc gtgcgccaca
      121 cgcggcgcat cgcctgcacg tcgatcaggc ccatcgcgac gatgaacagc acggcggcca
      181 tcgacgcgac cggcaggtag ccggcgagcg ggcgcacgag cagcaggatc acgagcagca
      241 gcaccgccga gaacacgcag gcgagcggcg tgcgcgcgcc ggcctcgtag ttcgcgccgg
      301 tgcggttgaa cgagccggac gtcggatagc tcgacgtgaa cgcgccgacg atgttcgcga
      361 gcccctggcc gatgaattcc tggttgccgt cgatgcgctg tcccgatttg agcgcaaccg
      421 cgcgcgcgct ggaaatcgcc tcggacaggc cgatgacggt gagcgcgagc gcgatcggtg
      481 ccagcgtttg ccacgtctgc agcgacatct cgggaaccga cagcggcggc accgccgacg
      541 gcagcgcgcc gatcgtcggc acgttcgcga caccggtgag cgacagcacc cacgcgcaga
      601 gcgcgccggc gacgatgccg gtcagcatgt acggcacgcg cggcagcagg cgccttccga
      661 cgatcgcggc aacgagcgtg aacacgccga cgccgagcac ggcggggtcg gttgcgccga
      721 ggtgcgtgac gaaatcgcgc aggcccgcga tgaagctcgc ttgctgcggc acgtcgatgc
      781 cgaagaaatt gcgcagttgc gcgccgatga tcagcaggcc cgcgccggcg gtgaagccga
      841 tcacgacgga cgtcgagatg aagttgacga gcgatcccag gcgtgcgacg ccgagcccga
      901 gcttcacgat gccgaccatc aggttgagcg tcagcacgat ggcgacgtac gccggcgtga
      961 acggcgccgc aagcgcgctg acggtggcga acaccatcag cgacgtggcg ttcgtcggcc
     1021 ccgacatcag atgccacgac gagccccaca gcgcgccgac gatcgcgggg agcatcgcac
     1081 cgtagaggcc gtattcgggc ggcatgccgg caagcgtcgc gtaggcgacg ccctgcggca
     1141 gcaccatcac cgcgccgacg aggccggcga gcacgtcggc gcgcagcgtg acgcggttga
     1201 cgcggtggcg ccacgcaagg aacgggaaga ccttcagcgc gaacggcgac atcggaccgc
     1261 tagaagggcc ggttcggaag cgtggcgatc agcaccgagg tcgaagcgcg cagcgagccc
     1321 cgaaacactt cagaccttgc ccttccaggg cacgaccaga tgctcgacgt agcgcatcag
     1381 gagatcgaaa aggtaggcga cgacgccgat gacgatgatg cccatgatga cgacgtcggt
     1441 ccgcagaaag ttcgacgcgt tcagcaccat ctgtccgaga cccactgtag ccgccaccat
     1501 ttccgccgcg acgagcgtcg tccagccgaa gccgatcgcg atccgcatgc cggtgaggat
     1561 ctcgggcatc gcggcgggaa tcacgacgtg gaagatcacc tgccatttcg aaccgcccat
     1621 cgagtacgcc gcgttgatct gctcgatcgt cacgctgcgc acgcccgcgc gcgccgccat
     1681 tgcgagcggc gcgaagcagg cgaggtagat cagcacgacc ttcgccgtct cgtcgatgcc
     1741 gaaccagatc acgatcagcg gcaggtacgc gagcggcggc agcggccggt agaactcgat
     1801 cggcgggtcg aagatgccgc gcgcgatccg tgaaacgccc atggcgacgc cgacggggac
     1861 ggccgtcagc accgcaagca cgaacgcggc gaagacgcga tagaggctcc accagaagtg
     1921 ctcggccaac ggcttgccgc cctggatgtc gccgcgccac gcttcgccga agctcgtgac
     1981 gatcttctcc ggcgtaggca ggaacagatc cttgatccag ccgaagtgcg ttgcgaccca
     2041 ccagaacgcg aggagcccga cgacggtgac gacgctgatc agcgtgctcg gaccttcacc
     2101 aggcaggctg tagcccttga tcgtgcgcat gccgctcgcg gacgttgcgg cgtcatgcga
     2161 cgacgccacg gacgccgtgc cggacgcgcc atccgattgc gggatgtcgg ccgcgggggt
     2221 ggggatcgag ctcatgtcag tggcgccggg ccgccccaag ccactggcgc gcccccgttg
     2281 ggggcagcga gcggagcgag cgtgggggtc gtttcatcag cgatcaacgc tacgccgccg
     2341 cctcggcatc cgcaggtgcc gaatggatga gctgcagcac ttcctcgcgc acggcgatga
     2401 acctcgggtc ggccttgacc ttgcgcgcgt cacggcactc gacgaacttc cggccgaaat
     2461 cgagggtata gcgatgcgcg atgcggccgg gggagggcgt catgacgatg agctcggtcg
     2521 cgaggaacag cgcttcctcc acgctgtgcg tgatgaagaa gaacatcttg cgcgtcgttt
     2581 gccacaggta aaggatcagt tcctgctgct gctcccgggt gagcgcgtcg agggcgccaa
     2641 gcggctcgtc catcagcatc acttcgggat cgctcgcgag cgcacgcgcg agcccgacgc
     2701 gctgctgcat gccgcccgag atctggtaga ccggataggt cacgaacttc tcgagcccga
     2761 cgagccgcag cttgtcgagc gcgacggcgc gccgctcccc tttcggcacg ccgcgcatcc
     2821 gcaagccgaa ctcgacgttc tcgacgacgt tgagccatgg catcagcgcg tgcttctgga
     2881 acacgacgcc acgttccgcg ccggggccga tcaccggcct gccgtcgagg acgatctcgc
     2941 cggcggaagg ctgcatgaag ccggcgatgc acgacaagag cgtcgtcttc ccgcagcctg
     3001 aagcgccgag tgcgacgacg aagtcgcccg ggtcgagacc gaggttgacg ttcgacagcg
     3061 cgagcgtggt gcggccggag ctgccggcgt acttgacgct gacgttgcgg atttcgagct
     3121 tgctcaagag agggctgccg gtgacccaac agcccgcaag gttaccgcga cggcgcgcga
     3181 tgcgcgagca aaagcgcgtt atctcctcaa tcgtccgcgt gccggtcgcc gctgccggcg
     3241 gcgggtacgg tgcgcaggat caatgaccga acgaggacgc cggcgctcga aaggcgcttg
     3301 cgccggtgcc cggcacgttg ccgggttgtg ccggcgcgcc ggctggcggt gcagcggacc
     3361 agtcgggcgg cgtcggcgtt cgtgcctgcg tttgcgcgtc cgacgtaatg tcgaagctga
     3421 cgccgtcgct ccaggcgcca gtcggcgatg tccgcacacg gagcagcgtc ggtccggcaa
     3481 acgttccgct gatgcttcgc ggcacgactg ccccctgcgt caattgcgcg agcggcacct
     3541 gccatgacgc gacctttgcg gtgcccgatg ccgcctgcgc gcggctggcg gcgcgaggcg
     3601 caagcgcgct gagctcgacc gtcgcgacgc cattcgacgt ttcggcgccc agcgctgcgt
     3661 cgcgaatgcg aacgcgaatc tgcccttgcc gcacgctgct tccgggggct ggctcgagga
     3721 tcgcgatcgt gcgcatcgcg gcgcccgtcg ctggcgctga atgggatgcg ccggtcgccg
     3781 gctccggtgt gcgaatcgtc gttcctcccg cccgcaatgc cgacaacgcg ccgacttcct
     3841 tgctgcgcgg cgacggcgcg agcgcagtgc cgcccgccga catcgtctgc gaaggcgctg
     3901 cattcgccct gccggagtcc agcacgacgc ccgtcccggg atgctcctcg acgcgctcgt
     3961 gcagacgatg gatcgaagcc agatattgct gcgtgaccgc tgcacgctgc gcgtccgtca
     4021 tatcggacgt gaaggggcgc cggcgtttgc gcacgaggct gtcgacgaag tattgtggga
     4081 tcgtttcggt ggcgcttacg ctcgttacgc cgcaatcgat cgtcgccttc tgccagggat
     4141 ccgcgccgca gctcagcgtc gacgtgatcg tgccgtagta gtcatgcgtt gggatttcga
     4201 gcccgccctt gacgatgcga atcgtctcgc tggcttcgtt agtcgatgcg tcgtaacgac
     4261 ccaccgcgct gaacggaacg tgcacgggat acgcatcgcc acgcccgttg gtctcgtcgc
     4321 agtggccggt cgcgttgtac cagttgtcgg tgtactggat gcgctcgatc cagcagcgct
     4381 cttgcgcctc gggtgcgtag atcaggacgc ccaccgccga tgatgactgc gggaccgcga
     4441 cgagcgcgca tgttgcggaa atcgcggcga gcaggttgaa cgcactcata cggtgatttg
     4501 ccacggtgtc ctcgcttttc gcgatcgagc ttgcgtgcgg cgcggcgttc acggcgtgat
     4561 gccgaccggg ccgtacacgg tgagcgacgt gatcgcatac ccggagaggc agatgtcgtg
     4621 cgggccgctc gtgtggatca ccggcttcgg cggcgtcgtg aacggcactg gcaccgatgt
     4681 gcccggaatt tttccgaggt tgaacaccgc ggtgccgaag gcggtgatca tcgggaactc
     4741 gagcggatcg aagaactcga cgtcgagcga gaagccggga gacgtatggc tgcggaagat
     4801 gccccagctc accggcagcg cgctgacgaa cgtcgccggc gcaccgatcg atccgtcgcc
     4861 ggtatagcgc gaaacgactt cgtcgcgcca ctcgccgaag gcgtagccgg gggaatagac
     4921 gcgaccgcca gcgttgccgg ccatgtcgcg atcgccgctg tcggttcgcg catggaccat
     4981 cgaggcgccc acgacctcga agccgttgac gttgcgaaat ctaagcgtgt cggtgccggt
     5041 gcgcgggcag tcgatgctct cgccgtcgat ccagcgggcg acggcgttct tcgcgatgct
     5101 cgcctgtggc cacgtcggcc cggcgtcgac atgcaggtat tgcgcgatgc ggtcggcgtc
     5161 cttgaccgtc gcctccgcgc gagccgcgac gaacgtgatg ccgtcgaacc gatacgtggc
     5221 gccgctcgcg gtcttcactt cgagcaccgc cttcggctcg tccggcacgc cgcgcgtgcc
     5281 gccggggatg ttcgcttcga tcgcgccggc gttccatttc gcgaccagca acggcacggg
     5341 gttggacgcg agcgcgcctc ccgtgagacg cgcgccgccc ggccgatcgc cgagcgcatt
     5401 gccggcgatg acaagcttgc cgtccggcat gacgcgtatg tcctggatgc ggccgttgac
     5461 gctgacgatg cccgggtcgc cgactgcgtt cggccgcgtc ccgacggacg tacgaagcgc
     5521 ggcgggattc gccggaaaat tcgtcgcggc ggtcgcgctc gcggccaccg tcgggttctg
     5581 cgacggcgga cggaaccctg cggcgaccgc gtcctgcccc tcgcgcagtt gcgcgtattc
     5641 gcgctgcagg ttcgctcgct tcttcgcgac gatgctcaga tgctcgttcg cgacgaggct
     5701 cacgcgcgcg ggacgcttcg ctccctgcgt cgcgccggcg ctggttgcgc cggcggccgt
     5761 tacacctgcg gtggttgcgc ccgtcgatgg acgcgttgcg ggcgggtcga cgcccgcgtt
     5821 ggtcgcggcc gccatgccct tcaccgaggg cggcttcgtc gtgccgagct cgcgcttcag
     5881 cgtcgcggcc ggtgtctgcc tgccgccgac gatgatgatc gcgtggtcgt cgagcgcgag
     5941 cacgccgcgc ttcacgtcgg ccgcattcac ctgcttgccg ccgacgatga tgatcgcgtt
     6001 gtcgtcgagg tgcgcgagat cggccggcag gttcgcctgt tgcaggtcgt gcgccgggag
     6061 cacggcggaa gcgccgttcg ccgcggccgc gcgtggcgtc cacgccggca tcgcgccggc
     6121 ggatgcaggc gccgaaggcg caccgccgcc gtgcagcgaa gaggcgggag agcccgtgtc
     6181 gacgcctgcg ccctggcgca ggccgctcgc gggaaactgc gcgtgggcga gaacgaccac
     6241 ggcggatgcg ccgaaggcga cgagcgccgt gcatgtcatg cgggaccgac ggttcatcga
     6301 agcgtctcct tgcgacttct cagaggctcg tcattggatg tccgaacgcc gaagccggcg
     6361 cttggcaacg acgtcgtcgc gctgcccggc gtcgcggcga ccacgttgaa cgcgacgatg
     6421 tcctgccacg tcgacgttcc actcggccgc accttgagcg tcgcgcggcc gttcgtgcaa
     6481 ctgcagatct tgtccgcggg cacgacgaat ccggcggcga gctgtccgaa cgagacctgc
     6541 caggtcttgc tgccttgcac ggtcaccaac gatgcctgca tcggcgcacg cgatcgcgaa
     6601 cgatgcgtac aacgcgcgcg atgccgaacg aagagatgcg ttcatgtacg ccacgatcgc
     6661 tcccccaggt cagtgtccga agcgttcacc gagcgtcgct actgcggctt cgatcgccga
     6721 gcgcagcgcc tcgtcgacgc tttcgcggtg ttgctgctgg acgtggacgt cggggacgac
     6781 cggaatgccg tatccgcgcg agcctgcgac ggccgacagc aggttgccga cgccggagag
     6841 gctcgacgac gtggcggtga cgctgccctg cgcggtgacg acgtcgagca cttcgccgct
     6901 cgacgcgtcg acgacgcgaa cgtcgatcgc gagcgtgtcg cgattgctgc cgccgccgac
     6961 ttccatgccg ccgacgccga tcccgctcgc gcgctgatcc tcgctggcgt tggcttgtga
     7021 caccgagcct tcgaacacga agcgcgcgcc ttgaagctgc gtgctgccga cgtcgccggt
     7081 cgcgagcccg ttcgcgttga gctgcttttc gcgcacgacg ccttcgttca gccgcgaccg
     7141 ctcgacgacc cggaagcggc cgctcttgac cagcgcggtg acgaacatgt cggtcgcccc
     7201 tcgcgcgccg atctccgaga cgccggaacg gaactcgtag atcgtgacga ccggtcgcga
     7261 cgcgcggtcg gcggccgtcg cgggcggcgc tgcgccgact gctcgcgcgt cggccgccag
     7321 cgccggcatc gacgcggcga gcgcgactgc gacgagcatc ctgccgatcc gatatctagc
     7381 atccatggcc atgtttcccg ggagagccgc atccgatccc gacggatgcc cgcgatttca
     7441 gaacgcgccc ttgcaatcgc cgccgatcca gcgggccttc tgctgcatgt gcatgtcgtt
     7501 gcctgtcccc cgggacgtca tgtcgactgc atagtggtcg gccgatatgt catagacgcg
     7561 cgtgtcgacc tcacgagtgc cgcctttagg gttgcggcaa gtggcgtgaa tgcggacttc
     7621 actcaccgac tcggagagaa ttttcgactt gcaattgctc tcgtcattca ggcccttcag
     7681 gtaaccactg gacgattgct cggcgagatc ttccggtgtc aggcagaagc gcatttgtcc
     7741 caccggcgcg ccgccgggcc ccgtgccgcg ctgcttcatg agtgcctcca cgcgagcccg
     7801 cttctcgggc ggcatgctcg cgagctcgtt tcggaagcgc tcctgctcgg cctgcatcgc
     7861 aggaccctca gccgtgtact gaagctccca caggcccggc ttgcgctgct tcaagccctg
     7921 cgcatccgcg gcgcatgcga tcgacagcag gacgagcgac gacaagagct ttccaaacgt
     7981 catggcaatc tcctccgaga ggcgcgcatc ggcagaacgg gggaagcgca tgttcggcgg
     8041 cccgtcaatc gcgtgtcggc tgttcgtcgt tcccatccgc cgccccttgc acggccgcgc
     8101 cgcgatgccg tcggctcgtc agcagtcggg tcaggagctg acgcgcgtcc gagaacgcga
     8161 cacggtcgcc cgattcgacg tgctccaccg tgccggccgg ctcttccgcg ccgcgcgcga
     8221 ccttgcgata gatgcgaacg atgaacgtat ccatccgtgc cgacattcac cggtcgaggc
     8281 aaatggaagc cgatacccgg acgcgagatt gcgcgccggc atctaacggc ggactaacgg
     8341 gaccgaaaaa ccgcgacgac cgaagcgaag ctgcggttgg ctaccgtgct ccgagagtgg
     8401 caacgagcgc gagggtgtcg gcggacggcc gaaccgacag cacgatcgac aactgctccc
     8461 ggcatcggcg gaacgcccgc atcgcgtcgg catggcgacc ctgcgcgatg tggcatcgaa
     8521 tggtgccttg atagagctct tcggcgagcg agtcctccgc gagcgcggcc tcgtagaccg
     8581 ccagcgcgtc ggcgaatcgg cgctccgatt cgagctcgcg acctcgctct acggttcgcc
     8641 gcaggaaccg ctggtgcaac cgctcgcgcg cggacgccca ccatggagcg acggcctcgt
     8701 gaccgaagag cggcccggca tagtgctcgt tctcgccgaa ggcgaacgcg taggcgtcga
     8761 tccagacaaa cgcaggattg aagccgatgc gcccggcatc gagcgtcagc gcatcgtcgc
     8821 ggccgaggag cttccgcagc cgcatcagcg tcatgtcgag cgacgcgcgc gccgcgccgc
     8881 cctcggcgtc cggccacagc gcatcgagga caagcgccgc gtcgacggga gcgggcccgt
     8941 gcgcgacgat cgctttcacg aggtcgagcg gcttgcgctg ggcacgcgca ccgaacacga
     9001 gcggctggcc ctcgacgacg atgcgcaagc cgccgagcgc atggatgcgc acgggccacg
     9061 gccagttcgc gtcgtacggc gagggcgcgg gcagccgctt gtcgcggatc agacggcgcg
     9121 cgaagtcggg ttcgatgccg aggcgcagcg cttcggagca gatgcgcgcg acgaccgggc
     9181 gcaacagcat ggcgatgccg cgccagttgt aggcacgcgt ccgttccatg atctttgcga
     9241 cgagcggtgc aggaatcgcg ccgtgcgctt tccactcgat gtcggcgagg gcgacgtcgg
     9301 cgaggaccac cgcgaagtcg cgccgcaagc cttccggata gccgggagca tcgcgcagga
     9361 gcccttcgcg tgcacgggcg gcctctccgc tcgcgatgag gacgcgatag cgggagagcg
     9421 accggttcca gtagtcgtcc tcgggatgcg cgtgctcgcg cgccagcgca tcggcgcgct
     9481 cgaacgtcgc gatcgatcgc cgccattcgc cggcgagccc gagcaccgcc gcttcggtcg
     9541 caagcagcgt gacggtctgc acgacgtgcc ggggattggc gctttcgcgc tgccgctcga
     9601 gccaatgccg ggcacgcggc aggtcgttgt cgccgatagc gccatggcac aggaaatccg
     9661 cggcgatgaa tgcgagcgcg tcgccgccgt gcgaccgcgc gagatcgtac gcgagctcga
     9721 gactcggatt gccccccgcg gcgggcagcc tgacgcccgg cgacggccag tgctttccga
     9781 gccccgcgta cgaattgagc cacatcgcct tcacgagcgg tgccagatcc ggatgctgga
     9841 gccacgccgc ggtcgccgtg atggtcgcct ggaagcgcgc ttcgtcgtgt agattgtagc
     9901 cgctcgattc gaccagcacg gccgacgtcg cgacgatctc gtcgactgaa acaccgggcg
     9961 gcggatcgcg cacctgcgcg aggacgcgat cgaacagcgc gggccggtcg cacagcgaat
    10021 cgtcccagag ctggtcggcg gtctgcagcc acgtgcggtc gatgcgaagc acgtcgtgcg
    10081 gatcggtgat ccgctcgcgg gcgcccaggt ggcgacgcag ccgatcggcc cacgttcgcg
    10141 tgccgtcgta gctctgccag ccttcgaaga tcgcgttcag cgcgctcgac gcgagcagca
    10201 aaccgtcggc cgacggcgtt tcatcgtcga tcatcgcgta cgcgcgctcg aagcaggcga
    10261 ccgccgagct cgcgtggttg cgttgccatg cgctttcgcc cagggcgatc tgcagcacgc
    10321 ggctgccatg cgcttcttcg ggcgacagtg cggcaacgac cgcgtcgagc tcggcgaggc
    10381 gatgagcgcg tacgaggctt cgcgcatgct cttccgcgat gcctttggca cgggatccct
    10441 cgttggcgtc gagatacatt tgcaccgcgt cgcgaacgtg accggcggcg gctgcgagcg
    10501 gtgcggcagc gagcagcgcc gtccgcctcc acgctgcgtc ggcgatcgtg cgaaagcgcg
    10561 ctcgcagcgc gtcgcgcaga agatcgtgca ggcgccagct cgcagggccg cgttcgagac
    10621 gaacgacgaa ggcgttttcg cgccgcaggc gctcgagcag ttggccggcg ccgccgtcga
    10681 gaccgagtcg agccagcgag gcagcgtcga cttccggcag aaggctgacc accgccaagg
    10741 tgcgaagctc ggaatcgtcg aagaccgcga gcacccgttc gtcgaagtag gccgcgatgc
    10801 gcgcgctcgc cgcgtgacgg gccggcgcac tttcgcccga ttgcgcgagc aacagcatgc
    10861 cagcggccca gccgtcggtg cgcgcgtgca gtcggcgcgc ggttgcggcg tccatgcgat
    10921 gggcgagcag cgagaccgcc tccgacgcgt cgaacgcgag cgccgccgtg tcgagcacat
    10981 ggacgcggcc gcttgcaaca tcgtcgagca gcgggccggc cggttcgcgt cggctcagga
    11041 cgacgaccgc gacgtcgggc ggcgcctcgc ggatcgccgc cgcaagcagc gtcaggaacg
    11101 catcgccgct cgctgcgtgc gcatcgtcga cgacgagcgt cgaagccgcc ggcacgacgc
    11161 gaaagaacgt gcggaagaac gccgtcgcga acaggtccag gcgatcgatg tcgcgcggac
    11221 ggtaggaggg caatccctgg gcgcccttcg acgtgccggc gagctgccgg aaatagccga
    11281 acgcttcggc gggatccgcg tctgctgcgt ccgcgcggaa ccagagcagc cttcccgcgc
    11341 gcggcgtggc ccaggtgacg gcgagtgtgc tcttgccggt ccccgggccg gcggcgatga
    11401 aggcagcgcc acgctcgacg gcttcgtcga gcagccgatg caggcgcgca cgcacgagcg
    11461 cggtaccggt gcgcggcgcc gagagcttgg cgaggcgagg cgaactcggc atgcggagcg
    11521 aaggctacat gaatcgggac ggcaaaaaaa tcgggacgtg cgcgacgtcc cgatcgtttg
    11581 cacccgcgcg cgtggcggtc gctatttcat cgcgtccttg acgtactggt cggtaaccac
    11641 ggtcgaataa tcgggcagcg tcttctcgat ctgcttttgc gacagctcga acttcgacgt
    11701 tgcctcgagc gccttcgccg cgacgctgct cttgccgccg cccagccatt tgctgttctg
    11761 ctcggcgagc gtcggaaagc gatagagcgc catgccttcg ggcacgctct caggcttggc
    11821 gcccgaccac ttcgccaccg ccttgacctc gggcgaatca ggcgtccact tcgccttgtt
    11881 cgtgcggtac tgctcgtcgg ccgcggcgag caccttgacg aatttcacca tgaagtcgtg
    11941 gtgctgcttc gcccagtcct tgttgacgac gtagccgtcg aacgtcgcct tgcccgtttc
    12001 ggcggaaagc ttgcccgacg tcaggatgac cttgccgtcc ttcttcacct cggagagcac
    12061 cgggtcccag atccacgtcg cctggatgtc gccgcgctgc caggccgcgc gcacttcggg
    12121 gggacgcatg ttgagaatct gcacgtcctt cggattgatg cccgcctgct cgagcgcaac
    12181 catcgtgtgg aaatgcgtgg tcgagttgaa cggcatcgcg atcttcttcc ccttcagatc
    12241 cttgacgctg ttgatgcccg acccgttctt cgccacgagc gcctcggcgt cgccgatgtc
    12301 gtcgaggatc cagaagagct cgaacggctc gccacgcgac accgccgcgg cgatgcccgc
    12361 ggagcccacc tcgccgatct gcacggcgcc cgacgccatc gccttgatga cttcaccgcc
    12421 gccgccgaac tgtttccagt tgatcttgta gccggtggcc ttctcgagat ccttcgcgtc
    12481 ctgcgacacc cgatacgggt cgagcatgtc ctggtacgcg atggtgactt ccttctgctg
    12541 tgccgaagcg atccccgcgc cgaacgcaag cgccaatccg atgaaccaga ctttcctcat
    12601 gccggtctcc ctgttttgat ggtccgcgaa gactaaagcg cggataccgt tttgcgcaag
    12661 atgtcgacga tcgtgtcgac gtgctgccgc tccgaaatca gcggcggcgc gacgagggcg
    12721 cagtcgccgg tgttcttgag attgagcccg ttgtcgaaca gctttttctg cagcacgttg
    12781 ccgcgccggc cgggtgcgcc gtccgattcg acgtcgagag ccgcgaacat gccgtagccg
    12841 cggacatcgg tgacgttctt cgttcccttg agcgaaaaca tcgcgtcctg aaagtagggt
    12901 gcgagctcgc gcccgcgctc gaacaggcct tcgttgcggt agatgtcgag cgtggcgagg
    12961 ccggcagcgc acgcggccgg gtgtccggaa taggtgtagc cgtggaagaa ctcgatggcg
    13021 ccgtcctgcg cggcggccat taccgtgtcg tggatgcgct cggagatggc caccgcgccc
    13081 atcggctgcg cggcgttggt gagcgccttc gcaatcgtca tgatgtcggg cgtcacgccg
    13141 aagctttgcg cggcgaacgg ctgccccgtg cgtccgaagc cggtgatcac ctcgtcgaag
    13201 acgagcagga tgccgtgggt gtcgcagatc tcgcgcagcc gcgcgagata gcctttcggt
    13261 ggcaccaggc agccggttga gccggcgatc ggctcgacga agcaggcggc gatgttctcg
    13321 gcgccgtaca gattcacgaa gcgcaacagg tcgtcggcga gctctgcgcc atgcgcgccc
    13381 tcgcccggcg tgtaacgatt ctgctcgagc tgtgtgtgcc gcatgtgcgc gacgccggcg
    13441 atccccgggc cgtacttgcg ccggttgttg acgatgccgg agagtgcgac gccgccgaaa
    13501 ttgacgccgt gatacgcgcg ctcgcgcgac acgaacatcg tgcggccggt ctgcccgcgc
    13561 gcctggtgat aggcgagcgc cgccttcatc gcggtgtcga cggcctcgga gccggaattg
    13621 acgaagaaga tgcggttgag atcacccggg gtcagctcgg cgatgcgcga agcgagctcg
    13681 aagctcttgg gatgaccgcg caggaacggc gcgacgaagt cgagctcgcc gagctggcga
    13741 tgcacggcct cgctgatctc cttgcggcag tggcccgccg cgacgcagaa caggccggac
    13801 gacgcgtcga tgatcttctc gccgcggtcg ttgtagtaat acattccctc ggcgcgcacg
    13861 acgatgcgcg gatcggactt gaagtcgcgg ttcggcgtga acggcatcca cagctcgtcg
    13921 agcgagaggg gtttttgcgc aggctggttt gcaagggtga attcggcgtg gcccatggtt
    13981 ctcggaggtc agatgacaga ggacaggtaa cggaggctgg ggatcgcggc gcaccgcggc
    14041 ccctgccgcg ctggttcatc atatgccttt tagcgccaag ccttgccagg cacaaggagc
    14101 cagacgggac tgccgaactg ggccaggggt gctgtcgggg agtgcgcgga tcggcaaacc
    14161 gaagcagggt cagagacgac ttttcgtcgc cggccacttc tctcacccgc ttcgcctgaa
    14221 aagtcgtctc tgaccctcta tgtggaacca gctcatccag ctttcctttc gcagcaaggt
    14281 cgggctgcag gcgcagatcc gggagatgct cgtcgcggcg atcctcgacg gccatatccc
    14341 cgtcggcgtg gcgctgccgt cgacgcgcgt actggcggcg cagctcggcg ttgcgcgcaa
    14401 caccgtcgcg ctcgcctatg agctgctggt caacgaaggc tacctgctga cgaagagccg
    14461 cagcggccac tacgtcaacc ccgagatcgt cgccggccgc gttgcgctcc cgcgtgcacc
    14521 gggcgtgggc gacatgcccg cccaggtcga ttgggcgcca cgactgcgcg gcggcgtgct
    14581 gatgcagcgc aacatcgtga agccgcgcga ctggcagcgc tacccgtatc cgttcattta
    14641 cggacagttc gatccgggac tcctgcccat tcccggttgg cgcgaatgca gcctgcaggc
    14701 aacgtcggtg tcggaagtgc gcggctgggc gggcgacctg atcgacgccg acgacgcgct
    14761 tctgatcgag cagattcaga cgcgactcct ggtgcgtcgc ggcgtgttcg cggcacccga
    14821 cgaaatcctc gtgaccgtcg gcgcgcagca cgcgctgttc ctgctcgcga cgctgctcgt
    14881 cgacgccgcc accaccgtgg gcatcgagaa tccgggctat cccgacgcgc gcaacatttt
    14941 cgcgtcgcgg accgcgagca tcgtgccgct gccgctcgac gacgcgggtc tcgcactcgg
    15001 tcctgcgctc gagagctgcg aatacgtcta cctgacgccc tcgcatcaat gcccgacgaa
    15061 cgtcacgatg ccgctcgcac ggcgggaagc gctgcttgcg tgggcccacg cgaacgatcg
    15121 catcctgatc gaggacgact acgaaatcga gctcggcctc gagggtcacg cgcatcccgc
    15181 gctgacgagc ctcgaccgca gcggtcacgt cgtctatgtg agcagcctgt cgaagacgct
    15241 cgcgcccggc attcgcagcg gcttcatcgt cggccgccgc gagctcatcc gcgaactgcg
    15301 tgcgttgcgg cgcctgatgc tgcgccatcc ggcggcgaac aacgagcgct cggtcggcct
    15361 cttcctcgcg atgggtcacc acgatgcgtt gctgcggcgc ctgcgccagg cgtacgccga
    15421 gcgatcgcgg gtgatggcgg ccgcgctcgc gacgcatctt cccggcgttc gcttctcgac
    15481 cgtgtccggc gcatcgtcgt tctggttgcg ttttccgacg cacgtcgaca cgcgggtgct
    15541 cgccgatgcc gcggcgctca agggcgtgct gatcgagccc ggcgacgtgt ttttctccgg
    15601 tggcggcgag cctgcgccgc gcaattacgc ccgcctcggc tttgcgtcga tcgacgccgc
    15661 gcgcatcgac gccggcatcg cgacgcttgc cggcgcgtgc gccgaagtgg ccgccgcgtc
    15721 cgcctgacac cacaccggcg catccgctgg ccctatcggc gtcgtcgcgc cgcgcgtaat
    15781 ctcacgcccg ttccgttccg cgaggaagcg cgcatgcgca ttggcgtccc gaaggaaatc
    15841 aaggtgctgg agaaccgcgt tggcctcaca cccggctccg tgcgcgagct cgtgcatcgc
    15901 ggacacgagc tcgtgatgca ggacggagcg gggcagggca tcggcgtcgc caatgccgac
    15961 tacgcgcgcg aaggcgcgac gattgcgcaa agcgccgcgg atgtcttttc gtcgtgcgac
    16021 atgatcgtga aggtgaagga gccgcaggcg gcagagcgcg cgatgctgcg gccgggacag
    16081 atcctcttca cgtacctgca tctggcgccc gatcctgcgc agacgcgcga tctcgtcgcg
    16141 agcaacgcgg tttgcatcgc ctacgaaacg gtgacgtcgc ccgccggcgg gctaccgctg
    16201 ctcgcgccga tgtccgaggt ggccggccgg ctcgcggtgc aggcaggcgc gcactatctc
    16261 gagaagccgc acggcgggaa gggcattttg ctcggcggcg tgccgggcgt tgcgccggcg
    16321 tcggtcgtgg tgatcggcgg tggcgtggtc ggcacgcatg cgatcgagat cgcgctcggc
    16381 atgggcgccc gcgtcaccgt gctcgaccgg agcgtcgatg cgttgaaacg cctgtggacg
    16441 cagtacggcg cggcgctgca gacgctgttc tccaccgtcg acgcgatcga gcgccaggtc
    16501 gcggcggccg acctcgtcgt cggcggcgtg ctcgtggccg gtgccgcggc gccgaagctc
    16561 gtgacgcgcg cgatggtcgc gaagatgcag ccgggatcgg tcgtcgtcga cgtcgccatc
    16621 gaccagggcg gctgcttcga gacgtcgcat ccgacgacgc acgccgagcc cacgtacgtc
    16681 gtcgacggcg tcgtccacta ctgcgtcgcg aacatgccgg gcggcgttcc gcgcacgtcg
    16741 acgttcgcgc tcaacaacgc gacgctgccg ttcgtggtgg ccatcgcgga caagggctgg
    16801 aagcgcgcgc tcgccgagga cgcgcacctt cagcagggac tgaacgtcgc gttgggcagg
    16861 gtgacgtgcc gccccgtggc cgacgcgctc ggttacgcgt ggaccgacgc agcctcgttc
    16921 ctggcgtgag gcagcgcctg cagcggccga cgcttattga gcataacgga actccatgac
    16981 aatttggaga gcaccgtcgt tcccgcggaa gcgggaacct agcgtctttc gctcggacgc
    17041 gccactgggt ccccgccttc gcggggacga cgaagcttcg gagcctcacg tcagataatt
    17101 atgtagatct caacaggcga tcgcgatcgt gcgtcccgtt gcgtccgatt cgcgaatcgc
    17161 atccagcact tgctggcagc gcaggccgtc ggtgaaattc ggcgacggcg acgtgcccgc
    17221 atcgatgccg cgggtgaaat cgcgaacgag cagccggaat gccatcagcc gagggtcgcg
    17281 tccgtcggtg aacggcgtga attgcgacgg agtttcgagc ggccgtagcg gttcgccatc
    17341 gcggcttgcg atgacgcgcc cgtcttccgg gggatttggc cccgcctgct cggcgatcag
    17401 cgttccgcga tcgcccatca cagcgatcct cgcgccgcgc gcgggtgtcg ctgcgaacga
    17461 gcacgtcatc gtcgccatcc ccccgttcgc gaacgcgagc gtgaacacga acgcgtcgtc
    17521 ggtttcgcct tgcagctctc gccccgttgc cggatcgacg agatcgggac gcagcgtcat
    17581 gaggcgcccg cttgccgaga cgacgtcgcc gaaccagtct cgcagcccgt cgatgtaatg
    17641 cgacccgagt gccccgagaa cgccgccccc ttcggaacga aacgcctgcc acgtcagcgc
    17701 gcgcggctcg cgcgtgacgt agcggtcgag gaagagctcg accgtgcaaa gctggaagcg
    17761 gccgatgtag ccgtcgtcga gcagctgctt gatgtagcgg cgctgcggcg catggcggaa
    17821 ttcgtggccc accatcgcgg ttttcccgct gcgcgccgcg gcccgtgtca tcgcgatcgc
    17881 ctgcccggca tcgagcgcga acggcttttc gcacattacg tgcttgcccg cgtcgagcgc
    17941 ggcaatcgca agggcgcagt gcgtgcccgg cggagtcgcg atgccgattg ccgaaaggtc
    18001 gtcgcggccg atgagctcga gcgggtcggt gtggatgtcg cggatacccg cttcgtcggc
    18061 ggccttgcgc gtccggtccg catgccggct gcaaagcgcc gcgacttccc agccctcgga
    18121 ggcaaagccg gggacgtacg cttgcatgcc gaagccgatg ccgatgatgc cgatccgccg
    18181 attcgagctc gccatgaaac ctccggagtc tgttgcgacg gcagctgagg ggacgcgcgg
    18241 ccggcgatcg caatgggccg cgagctgcgc cgaaggataa tgccacggcg tgctggactc
    18301 aatgttgccg ttgctctggc cctacccccg cgtcgctcac ggcgtcacaa tcccacgatt
    18361 cgacgcctcg aggacgcaac gccatgaaca cgatgaccga ttccaggaat ctcgccgtgc
    18421 agggtcggca gaaaatgacg ccgtccgagg cgttcgtcga atcgctcgtt gcacagggcg
    18481 tcaaggacgt cttcggcatc gtcggctccg cgtacatgga tgcactgg
//
LOCUS       DASSVX010000338         8376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1398426, whole genome shotgun sequence.
ACCESSION   DASSVX010000338 DASSVX010000000
VERSION     DASSVX010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398426"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VFO53_14560"
     CDS             <1..572
                     /locus_tag="VFO53_14560"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8877547.1"
                     /translation="NWNWPDIADAFSQGTLGCYIDAHSSASVLVNPEKSKVVGKIAFA
                     RWPKGPTGKRCTSIWNWGFPINAALSDKQKRATWLFISWAASAETQARTSWKFAGPAK
                     RTGLNRTSLWKSPEFVALTKEIGPNFVDAALTSLQQDTDVDWRPRVPQWPAIGDTMAT
                     AIQSALVGQKKSKQALEEAQARIDQILKG"
     gene            1206..1418
                     /locus_tag="VFO53_14565"
     CDS             1206..1418
                     /locus_tag="VFO53_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877548.1"
                     /translation="MNYEPAMHRRPYRRGDFHFVARPQEGAYWRSREGVIVRVASGEG
                     ELPQARRSAPAWLGALAFLLARTARS"
     gene            1541..2119
                     /locus_tag="VFO53_14570"
     CDS             1541..2119
                     /locus_tag="VFO53_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET8877549.1"
                     /translation="MDPLVLTVDMAGLPQSWVALEEAITYHAKQAVAWSLGAVVREFR
                     GGFQRDGSRSRISTKSILAIKGSAGRPLAHTPGLTNPMLFARDRQVCAYCGGRFQYRE
                     LSRDHIIPASRGGRDTWMNSVTACRSCNTRKGNRAPEQADMPLLYVPYVPNRHEHFIL
                     RNRRILADQMEYLLAGVPRTSRLHPQPRAYAA"
     gene            2365..3543
                     /locus_tag="VFO53_14575"
     CDS             2365..3543
                     /locus_tag="VFO53_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET8877550.1"
                     /translation="MANENPSRENPARQQSAERAQTGTSQQGTGAQQGTSQGGSHGTG
                     GYGQGTGQGTQGRTGYAPGSSGQNRNSERGYEQGQGRYGQQGTGRGNYGQGVGTSTWQ
                     GGRQSGGPLSTRGGSSMSPYAGASGYGSGPFSVMRRISDEMDRLFENFGMGRGLFQGE
                     PEQGRAWDAGGYGAGAPSMWSPHIEVCERSGKLLIQADLPGMKRDDVHVRIEDDEVII
                     QGERRQEQAQDESGYYRSERSYGSFYRTIPLPEGTNAESASATFKDGVLEIEIDAPRP
                     QQRGRTLEIRDAGASQSGGRSGTGSGTTYGSGTSGAMEGTSGTSGGQHPQSGASASQH
                     ATAGTSGTQHSASGTSGTQQSSGSGTMYSGSGNPSVGAAGMGGSQASSSDKSSRGSST
                     "
     gene            3697..4326
                     /locus_tag="VFO53_14580"
     CDS             3697..4326
                     /locus_tag="VFO53_14580"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HET8877551.1"
                     /translation="MHVVTFAAPPAVRNPDDPRRANRAQIATWSVVAYLLLVLAAWSA
                     PSPEIADAPPQPEHGIKAAFLYKFLGYVEWPAVALNNATAPIVIGVLGADDIADELRT
                     IVARRRIAQHPIEVRRVDEADAVDGVNVLFIGRDDAAALRRLAPAAQARSVLLVSDSD
                     RGLEEGSVINLVVVDNRVRFEVSLEAAERSELKLSSRMLAVAMWVRPPR"
     gene            4349..6256
                     /locus_tag="VFO53_14585"
     CDS             4349..6256
                     /locus_tag="VFO53_14585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8877552.1"
                     /translation="MRNPGQSIRSKLMLVVLVTTATALVVVAIALVVYESRAYEQGSA
                     NALMTQAQILGRASAPALVFDDPVAARENLALLKVQPRIRAAAIYKPSGRLFASYGAS
                     DLFGPGFPRTGQADGYSIEGNEIVVYRRIVEGGEPLGTIFLRSSYELDARLRDYLGIL
                     GIVLIASLLLALLLSAALQRAITRPILAVTDVAQQVIERRDFSLRVAKTTHDEVGVLV
                     DAFNAMLAEVGRRSEALEASNAILSRETAERREAEEALRIANRRKDEFLAILAHELRN
                     PLAPLRNALEILKRSGGESTTARGAREMMERQLRQMVRLVDDLLDVSRITTGKLALRT
                     EIVELQDVIRSAIDTAKPLLEQRRHALGVVLPPEPLQVEADATRLSQVFSNLLNNAAN
                     YTEPGGLIEVRADASDSEITVSIRDNGIGIAPDMLGAVFRMFAQADASLERAQGGLGV
                     GLTLARHLIELHHGSIEAKSKGVGHGSEFIVRLPRAHGGLAPAVAVPHAPAARAQPTR
                     AGARRILIADDNIDFAMSLEAILKPLGHDVRVAHDGIAAQELAASFVPQIAFLDIGLP
                     GCNGYDLARNLRRLPQTAHARLVAITGWGQEDDRRRSRDAGFDVHLVKPVEPSQLVDI
                     IDTDIPLHAAV"
     gene            6347..7609
                     /locus_tag="VFO53_14590"
     CDS             6347..7609
                     /locus_tag="VFO53_14590"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF005536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET8877553.1"
                     /translation="MSSIDLPAPARHIRLTSHHPHEGAHAAARIQWGAADPLVRGPVV
                     GTTTERARRNVIGTHSGSYGVYRALAVAAKSLTRGHRPDLTNTSPTDSIGPYAQWYDA
                     DRIVSLDPWGGVVADVYKTFLANGDDIRPTIAITKAHIELPEVHEAIAAGRLVPDGKL
                     LFANGAAVVTKAAIEPVWWLPGIARRFNVGEADLRRALFEETGGMYPELVTRSDLEVL
                     LPPVGGLTVYIFGNPRDLADPAVTLTARVHDECNGSDVFGSDICTCRPYLTHAIEECI
                     RGAQTGGVGLVVYCRKEGRALGEVTKFLVYNARKRQAGGDRADNYFLRTECVAGVQDM
                     RFQQLMPDVLHWLGVRTIHRLVSMSNMKYDAIVASGIDVGERVRIPDDLVPADARVEI
                     DAKVAAGYFTDARVSGCDVPADAKGRPL"
     gene            7609..>8376
                     /locus_tag="VFO53_14595"
     CDS             7609..>8376
                     /locus_tag="VFO53_14595"
                     /inference="COORDINATES: protein motif:HMM:NF019570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1688 family protein"
                     /protein_id="HET8877554.1"
                     /translation="MLAVAPAAGADLLSASAIRERSANITRAVEAGSSTHFSVDRSRL
                     QPAARLVADVTRRRYRDLVIPYHSRWRHFDAGGRSRISELNRHLACYAPPDRLRAQID
                     LTVVSVLLDAGAGGAWRFREPGTIDDYTRSEGLGVASLRAFVGGAWSASPGEPLRVDA
                     AALTGIDAATLAHWFQVTPRNELVGLEGRAALLRRLGDVLETRPDRFGLTARPSALFD
                     SMLRGTTVSAPAILRALLDGFGDIWRGRRTNGHDASGD"
BASE COUNT         1355 a         2864 c         2858 g         1299 t
ORIGIN      
        1 gcaactggaa ctggcccgac atcgccgacg cgttctcgca aggcacgctc ggctgctaca
       61 tcgacgcgca ttcgtcggcg tccgtgctcg tcaacccgga gaagtcgaag gtcgtcggca
      121 agattgcatt cgcgcgctgg ccgaagggtc ccaccggaaa gcgctgcacg tcgatctgga
      181 actggggctt cccgatcaat gccgcacttt ccgacaagca gaagcgcgcg acatggctgt
      241 tcatctcgtg ggcggcgtcg gccgagacgc aggcgcgcac gtcgtggaaa ttcgccggtc
      301 cggcgaagcg caccgggctc aatcgcacgt cgctgtggaa gtcgcccgag ttcgttgcgc
      361 tgacgaagga gatcggaccc aatttcgtcg atgcggcgct gacgtcgttg cagcaggaca
      421 ccgacgtcga ctggcgcccg cgcgtgccgc aatggccggc gatcggcgac acgatggcca
      481 cggcgatcca gtcggcgctc gtcggacaga agaagtcgaa gcaggcgctc gaagaggcgc
      541 aggcgcgcat cgaccagatc ctgaagggat agggcgctgg ggtcagatac gattttccgg
      601 gtcgtttccg gaaaatcgtg tctgacccca tttcggttgt cgcacgactg ttgcatcgct
      661 gcacgccacg cctcactcgc atgcgtttcg gcgaggcttt ccttgtcgcg ggcgcactgg
      721 cgacgctaca atgcaagcac tcgcgagcgt tgacccgcgc atccgcattc acgacatgac
      781 ccacgaacgg cacatcagtt gcgcgatcac ctcggacccg agggtccgcg ctgtccgcgt
      841 cgccgtttcg caacgcctcg tgtcgtcgtg cgccgccctt tccggcggct tcgccgcgac
      901 gtaacgcctc ccggccttcg acgttcgccg ggcggccggc ggcaccgtcg aaaggtctcg
      961 cgattccttc gatcggtgtc atttgcggct tcgctgccgc gctggacgca cgcgcgcgtc
     1021 ttatcgacag gagcatcgac agcatgagcg acattttcca cacaggtctg caggcaagtg
     1081 ccaggagcgc tgtcggctga agcgacgcat cgtctttgtc gttgtgaagc ccggcacgca
     1141 tgaatggccg ggcttcgtcg ttctggcgcc cggattcgca cagaaaacat cgaacgaggc
     1201 gacacatgaa ttacgaacca gcgatgcacc ggcggcctta tcgacgcggc gattttcact
     1261 tcgtcgcacg gccgcaggaa ggcgcctatt ggcgatcgcg cgagggcgtc atcgtccgcg
     1321 tggcgagcgg tgaaggcgag ttgccgcagg cgcggcgcag cgcgcctgcg tggctgggcg
     1381 cgctcgcgtt cctgcttgcg cgcactgcaa ggtcgtgaac ccgtcggcgc aacggggccc
     1441 tgttgcgtta ataccgcacg cccaccccgg cgcggcgtcc gcaggtcggg cggagccgcg
     1501 ccggccggtc ggcgcgaccg actcgaatta caatccgccc atggacccgc tcgtcctcac
     1561 cgtcgacatg gccggcctgc cgcagtcctg ggtggccctc gaggaagcga tcacctatca
     1621 cgcgaagcag gcggtcgcgt ggtcgctcgg tgcggtcgtg cgcgaatttc gcggcggctt
     1681 ccagcgcgac ggctcgcgct cgcgcatctc gacgaagtcg atcctcgcca tcaagggcag
     1741 tgccgggcgg ccgctcgcgc acacgcccgg tctcaccaat ccgatgctgt tcgcacgtga
     1801 ccggcaggtg tgcgcgtact gcggcggccg ctttcagtat cgtgagctgt cgcgcgatca
     1861 catcatccct gcgtcgcgcg gcggccgcga cacgtggatg aattcggtca ccgcctgccg
     1921 cagctgcaat acgcgcaagg gcaaccgcgc gcccgagcag gccgatatgc cgctgctcta
     1981 cgtgccgtac gtgcccaacc ggcacgagca cttcatcctg cgcaaccgcc gcatcctcgc
     2041 cgaccagatg gagtacctgc tggcgggcgt gccgcgcacg agccgcctgc acccgcaacc
     2101 gcgcgcgtac gccgcctagc acgctggccg gcgcgagcgt acagcgctgc attgcgcgaa
     2161 gcgcatttgc ggaatcgttc aatcgcatcc gatcgcgatt tgcgatctgc acgaactctc
     2221 gtggcgtcgc gctttccaat cggtgggatt cgcgaccgcc gatgcgaccc gctcgttcca
     2281 ttccatagcg gtcgatcgcg gttgttctcg tggcgcccgg caccccgggc gattgcttcg
     2341 cagtcgaact tcgcaggaga catcatggcg aacgaaaatc cgtcgcgcga aaacccagca
     2401 cgacagcaaa gcgccgagcg cgcgcaaacc ggcacctccc agcagggcac gggtgcgcaa
     2461 cagggtacga gccagggcgg cagtcacggc accggcgggt acggccaggg aacggggcag
     2521 ggcacgcaag gacggaccgg ttacgcaccg ggaagcagcg gacagaatcg caacagcgag
     2581 cgcggttacg aacaggggca ggggcgttac ggacagcagg gcacgggtcg cggcaactac
     2641 ggtcagggtg tcggcacgtc cacgtggcag ggcggccgcc aatcgggcgg gccgctgtcc
     2701 acgcgcggcg gttcctcgat gtctccgtac gccggcgcgt cgggatacgg cagcggcccg
     2761 ttctcggtca tgcgccgcat cagcgacgag atggatcgcc tgttcgagaa cttcggcatg
     2821 ggtcgcgggt tgttccaggg cgagcccgag cagggacgcg cgtgggacgc gggcggttac
     2881 ggcgcgggcg cgccgtcgat gtggtcgccc cacatcgaag tgtgcgagcg cagcggcaag
     2941 ctgttgatcc aggccgacct gccggggatg aagcgcgacg acgtccacgt gcgcatcgag
     3001 gacgacgagg tgatcatcca gggcgagcgg cgacaggagc aggcgcagga cgagagcggc
     3061 tattaccgca gcgagcgcag ctacggcagc ttctaccgca cgattccgtt gcccgaaggc
     3121 acgaatgccg aatcggccag cgcgacgttc aaggatggcg tgctcgaaat cgagatcgac
     3181 gcgccgcgac cgcagcagcg cgggcgcacg ctcgaaatcc gcgacgccgg cgcgtcgcaa
     3241 tcgggcggtc gttccggcac gggcagcggc acgacctacg gcagtggcac gagcggcgcg
     3301 atggaaggca cgtcggggac gagcggcggg cagcacccgc agtcgggcgc gagcgcgtcg
     3361 cagcacgcga cggccggaac gagcggcacg cagcactcgg cttcgggcac gagcggcacg
     3421 cagcagtcgt cgggcagcgg tacgatgtac agcggatcgg gcaatccgtc ggtgggtgcc
     3481 gcgggcatgg gcggcagcca ggcaagctcg agtgacaagt cgtcgcgcgg ctcgagcacc
     3541 tgagccgacg tttcgttcga cgcggattgc ggggcgggcg gatgatcgac ccgcccattc
     3601 ttttggtgcc ggacgtcgcg ggcggcgctt tatactgcgc gatcggcggc cgattgccgc
     3661 gctttccgaa cggcagttca agcccgaggc ccgcaaatgc acgtcgtgac gttcgccgct
     3721 ccgccggccg ttcgcaaccc ggatgatccg cggcgcgcga accgcgcgca gatcgccacc
     3781 tggagcgtgg ttgcgtacct gcttctggtg ctcgctgcgt ggtcggcccc gtcgcccgaa
     3841 atcgccgatg ccccgccgca gcccgagcac ggcatcaagg ccgcgttcct ctacaaattc
     3901 ctcggctacg tcgaatggcc cgccgtggcg ctcaacaatg caacggcacc gatcgtcatc
     3961 ggcgtgcttg gcgccgacga catcgccgac gagctgcgca cgatcgtcgc ccggcggcgc
     4021 atcgcgcaac atccgatcga ggtccgccgc gtcgacgaag ccgacgcagt cgacggcgtg
     4081 aacgtgctgt tcatcggtcg cgatgacgcc gccgcgctgc gtcgcctcgc acccgccgca
     4141 caggcgcgct cggtactcct cgtcagcgac tccgaccgcg gcctcgagga aggcagcgtg
     4201 atcaatctcg tcgtagtcga caaccgcgtg cgcttcgagg tctcgctcga agccgccgag
     4261 cggagcgagc tcaagctgag ctcccgaatg ctggccgttg cgatgtgggt acggcctccc
     4321 cgctgaagcg gcccgcacga aggcgcggat gcgcaatccc gggcagtcga tacgcagcaa
     4381 gctgatgctc gtcgtgctgg tcacgacggc gactgcgctc gtcgtcgtcg cgattgcgct
     4441 cgtcgtctac gagtcgcgcg cttacgagca gggaagcgcg aacgcgctga tgacgcaggc
     4501 gcagatcctc ggccgcgcga gcgcccccgc gctggtgttc gacgatccgg tcgcggcgcg
     4561 cgaaaacctg gcgctcctca aggtgcagcc gcgcatccgc gccgccgcca tctacaagcc
     4621 cagcggccgg ctcttcgcga gctatggcgc aagcgacctg ttcggccccg gctttccgcg
     4681 cacgggacag gcggacggct attcgatcga gggcaacgag atcgtcgtct accggcgcat
     4741 cgtcgagggg ggcgaaccgc tcgggaccat tttcctgcgc tcgagctacg agctcgacgc
     4801 ccggctgcgc gattatctcg gcatcctcgg gatcgtgctg atcgcgagcc tgctgctcgc
     4861 gctgctgctg tcggccgcgc tgcagcgtgc gatcacgcga ccgatcctcg ccgtcaccga
     4921 tgtcgcgcag caggtgatcg agcggcgcga cttctcgttg cgcgtcgcca agaccacgca
     4981 cgacgaggtc ggcgtgctcg tcgacgcctt caacgcgatg ctcgccgaag tcggccggcg
     5041 ctcggaagcg ctcgaagcgt cgaacgcgat cctttcgcgc gaaaccgcgg agcggcgcga
     5101 agccgaggag gcgttgcgca tcgccaaccg gcgcaaggac gaattcctcg ccatcctcgc
     5161 gcacgagctg cgcaacccgc tcgcaccgct ccgcaacgcg ctcgagatcc tcaagcgcag
     5221 cggcggcgaa tcgacgaccg cgcgcggtgc ccgcgagatg atggagcgcc agctgcggca
     5281 gatggtgcgg ctcgtggacg acctgctcga cgtgtcacgc atcaccaccg gcaagctcgc
     5341 gctgcgcacc gagatcgtcg agttgcagga cgtgatcagg agcgcgatcg acaccgcgaa
     5401 gccgctgctc gagcagcgcc ggcacgcgct cggcgtggtg ctgccgcccg agccgctgca
     5461 ggtcgaggcc gatgcgacgc gcctgtcgca ggtgttctcg aaccttctca acaatgcagc
     5521 gaactacacc gagccgggtg gcctgatcga ggtgcgcgcg gacgccagcg acagcgagat
     5581 cacggtgtcg atccgcgaca atggcatcgg catcgcgccc gacatgctgg gggccgtctt
     5641 ccgcatgttc gcgcaggccg acgcgtcgct cgagcgggcg cagggcgggc tcggcgtggg
     5701 cctgacactg gcgcgccatc tgatcgagct gcatcacggg tcgatcgagg cgaagagcaa
     5761 aggcgtcggg cacggcagcg aattcatcgt gcgccttccg cgcgcgcatg gcggcctcgc
     5821 tcccgccgtc gccgtgccgc acgcgccggc ggcccgtgcg cagccgacgc gcgcaggcgc
     5881 acggcgcatc ctgatcgccg acgacaacat cgatttcgcg atgagtctcg aggcgatcct
     5941 gaagccgctc ggtcacgacg tgcgcgtcgc gcacgatggc atcgctgcgc aggagctcgc
     6001 cgcgagcttc gtgccgcaga ttgcgtttct cgatatcgga ctcccgggtt gcaatggcta
     6061 cgatctcgcg cgcaatctgc gccggttgcc gcagaccgcg cacgcgcggc tcgtcgcgat
     6121 caccgggtgg ggtcaggaag acgaccggcg acgctcgcgc gacgcgggtt tcgacgtgca
     6181 tctggtgaag ccggtcgagc cgtcgcaact cgtcgacatc atcgacaccg acattccgct
     6241 gcacgcggcg gtctgacgct cgtctgcaca tggcgcctgc tggcatggcg tttgcggcgt
     6301 cgtggatcat gacggggtat attcaagcgt tttgcgaaag acatcgatga gcagcatcga
     6361 cctgccggcc cctgcaaggc acatccggct cacgtcgcac catccgcacg agggtgcgca
     6421 cgccgcggcg cgaatccaat ggggcgccgc cgatccgctc gtgcgcggcc cggtcgtcgg
     6481 caccacgacc gagcgcgccc ggcgcaatgt gatcggaacg cacagcggca gctatggcgt
     6541 atatcgcgcg cttgccgtcg ccgcgaagtc gctgacgcgg gggcaccgcc ccgatctcac
     6601 gaatacatcg cccaccgata gcatcggacc gtacgcgcag tggtatgacg cagatcgcat
     6661 cgtctcgctc gatccatggg gcggcgtcgt cgctgacgtc tacaagacgt ttctcgcgaa
     6721 cggcgacgac atccggccga cgatcgccat caccaaggcg catatcgagc tgcccgaagt
     6781 gcacgaagcg atcgccgcgg gccggctggt ccccgacggg aagctgctgt ttgccaatgg
     6841 cgctgccgtg gtcaccaagg ctgcgatcga gccggtgtgg tggctgccgg gcattgcacg
     6901 gcggttcaac gtcggcgaag ccgatctgcg gcgtgcgcta ttcgaggaaa ccggcggcat
     6961 gtaccccgag ctcgtcacgc gcagcgatct cgaggtgctg ctgccgccgg tcggcgggct
     7021 gaccgtctac attttcggca atccgcgcga cctggccgat cctgcggtca cgctcaccgc
     7081 gcgcgtgcac gacgaatgca acgggtccga cgtgttcgga tcggacatct gcacctgccg
     7141 cccatacctg acgcacgcca tcgaggaatg catccgcggc gcgcagacgg gcggtgtcgg
     7201 tctcgtcgtc tattgccgca aggaggggcg cgcgctcggc gaggtgacaa agttcctggt
     7261 ctacaacgcg cgcaagcgcc aggcgggcgg cgaccgcgcc gacaactact tcctgcgcac
     7321 cgaatgcgtg gccggcgtgc aggacatgcg cttccagcag ctgatgcccg acgtgctgca
     7381 ctggctcggc gtgcgcacga ttcaccggct cgtgtcgatg agcaacatga aatacgacgc
     7441 gatcgtcgca tcgggcatcg acgtcggcga gcgggtgcgc attcccgacg acctggtccc
     7501 ggccgatgcg cgcgtcgaga tcgatgcgaa ggtggccgcc ggctatttca ccgacgcccg
     7561 cgtaagcggc tgcgacgtgc cggccgacgc gaaaggaagg ccgctctgat gcttgccgtc
     7621 gcacctgcgg ctggtgccga cctgctctcc gcttcggcga tccgcgagcg ttcggcgaac
     7681 ataacgcgcg ctgtcgaggc ggggagctcc acgcacttca gcgtcgaccg cagccggctg
     7741 cagccggcgg cacgcctcgt cgccgacgtg acgcggcgtc gctatcgcga tctcgtcatt
     7801 ccctatcaca gccgatggcg tcacttcgat gccggcgggc gcagccggat aagcgagctc
     7861 aaccgccatc tcgcctgcta cgcgccgccg gaccggttgc gcgcgcaaat cgatctcacg
     7921 gtcgtcagcg tgctgctcga cgccggcgcc ggtggcgcgt ggcgcttccg ggagccgggg
     7981 acgatcgacg actacacgcg ctccgagggg ctcggcgtgg cgagcttgcg cgcgttcgtc
     8041 gggggcgcct ggtcggcgtc gcccggcgag ccgcttcgcg tcgacgccgc agcgctgacc
     8101 ggcatcgacg cggcaacgct cgcgcactgg ttccaggtga cgccgcgcaa cgagctcgtg
     8161 ggactcgaag gtcgtgccgc cctgttgcgc cgcctgggcg acgtgctcga gacgcgtccc
     8221 gaccgcttcg ggttgaccgc gcgtccctcg gccctgttcg acagcatgct gcgcggcacg
     8281 accgtttccg cgccggcgat cctgcgcgcg ctgctcgatg gtttcggcga catctggcgc
     8341 ggacgccgga cgaacggcca cgacgcctcg ggcgac
//
LOCUS       DASSVX010000339         5417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1531470, whole genome shotgun sequence.
ACCESSION   DASSVX010000339 DASSVX010000000
VERSION     DASSVX010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531470"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /gene="gspG"
                     /locus_tag="VFO53_14600"
     CDS             <1..355
                     /gene="gspG"
                     /locus_tag="VFO53_14600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HET8877555.1"
                     /translation="VVLGIVGSNYLGKSEQAKAKAAKIEIEQISQALDLFKLELGRYP
                     TTQEGLQALVAAPPGLANWAGPYWKKSTTPKDPWSHEYKYASPGTHGAYDIISLGADG
                     VEGGEGANKDVTNWE"
     gene            391..840
                     /locus_tag="VFO53_14605"
     CDS             391..840
                     /locus_tag="VFO53_14605"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HET8877556.1"
                     /translation="MSVRRCSFGVTLLELLIVIALMAIVAGFVLPMFGGPVSTSELRA
                     TARQLAAGLRLARSEALAERRETFLVVDVAGRRFKVDRDPAEHKLSSKIDIKLFTAQN
                     DLVDNNIGSIRFFPDGGSNGGRITVASGERKFDVDVDWLTGRVAILD"
     gene            842..1273
                     /locus_tag="VFO53_14610"
     CDS             842..1273
                     /locus_tag="VFO53_14610"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET8877557.1"
                     /translation="MSDARRNPHRGFSLLEVLVAFVIVALVVTALFRLFGGALGNASA
                     ADEWTRALLVAQSRLALAASAQPLRETTDAGSEADGRIDWRTSVTPYVPPDGDPDVER
                     ASETMATRLFRVTVDVRFPGIAGQTRTLTLSTVKLGQRNPA"
     gene            1291..1968
                     /locus_tag="VFO53_14615"
     CDS             1291..1968
                     /locus_tag="VFO53_14615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET8877558.1"
                     /translation="MRTARTVRGFTLLELMIALTLLALLSAVLFGSLRLAGRSTDGGD
                     AKVEAAASMRLAQEFLRTNLEAQHPLRMRKIVDSPLLFSGTRNELRYAAELPPRVAGG
                     GIWFYRLSLRTEDSRGPLTLERVVPDLAADAIPEFANADRSVLADGIAELTLGYFGRD
                     TDAATTVAPTWRDHWDNVQQLPMMIRIDVKPKQGPAWPTLYVTPREAPEAGCRAWDIA
                     RQRCAAV"
     gene            1953..2927
                     /locus_tag="VFO53_14620"
     CDS             1953..2927
                     /locus_tag="VFO53_14620"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877559.1"
                     /translation="MRSGLITNRRPLRSRTRNAQSGIALVLALWLTVLLTVLASGFAF
                     SMRTEALAVRNAVSLAQARVIADGAVERTAYELMRPQIANVWKPDGQPHRFVDGEATV
                     TVTAVDEAAKIDMNSAPDPLLKNLLIVIGGLDEATAGNLVDAIDDWRDPDDLRRPNGA
                     ELPEYRAANLKYGPANAPFETIGEAARVLGMTADVYNRIAPAITVYSHQPGVNPATAS
                     RSVLLALPNATADTVDAFIAQRAQALADKLPVPPFPPAQAFGSGPVQIWRIRAEATLP
                     DGVTFAREAVLRPSPDAQRPLIALAWLEPTRSVAAPSLSEPTADGRRR"
     gene            2911..4395
                     /locus_tag="VFO53_14625"
     CDS             2911..4395
                     /locus_tag="VFO53_14625"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HET8877560.1"
                     /translation="MAAVADSTSFNKPGYARDVARRLGLTGFGHWWARELAALMPSRL
                     RAAVAHRHARPLLAFDGAQATLWRPVTSAGQVTMVEAARIPLDGDPATVAAGGRAALA
                     PLTRGANGGAPEVVIALSPRASLRKRLTLPAAIEPNLHQALAYDLDRHTPFKSGELYF
                     DAAIADRDPANNTLHVDLAAARRAIVDPMLRHAESFGARVVGITVDPPAIASTSRLDL
                     LPVEREDRTERSRWRVIVPLVLLAIAILIALVLPVWQKREEAIALNQLSDQARQRAGA
                     SDALRTELERKTSEYNFALERKYAFPGTVQVLDDVTHLLPDDTWLTQLEVRSVRGKEG
                     QRELTLRGESANAGRLVSLLEDSKLFTQAAPRSPTTKIQPGPGEIFDVGAQLKPAAMP
                     QPAPLDLTAPPPQAPQRARSNPATQPSTPATAATQAGAAAPAAAPAAAPTNPPPVPNR
                     RSVITRSAPPAPAAAPAGAAPQPAPGAPPVTAEPEPADGEGATQ"
     gene            4392..5015
                     /gene="gspM"
                     /locus_tag="VFO53_14630"
     CDS             4392..5015
                     /gene="gspM"
                     /locus_tag="VFO53_14630"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HET8877561.1"
                     /translation="MTPPALKGLTAAQQRALAAGLLFGGIAALLLIVLVPIVLLHRHY
                     DAAIADTSDKLQRYQRIAAQAPAFRTALDAMQQRDGRRFFLRNTASNLAGAELQELVK
                     AAIEGNGGRITTSQNTSPREDGRFREIGVNVQFFATTPALQRILLAIETHQPYLIVEN
                     LTARPLNAFRGFKPAPGQEPELNVQLDVGAWAFPEPARQTAPPKAAS"
     gene            5041..>5417
                     /locus_tag="VFO53_14635"
     CDS             5041..>5417
                     /locus_tag="VFO53_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877562.1"
                     /translation="MNDASALARTLIVWIVPFAIAVAAIGIETDWGRSLNRDTPAPTA
                     VTPQPVAVALLPEYRIDGGTEARRETVDRVLFNPTRRPAPPANQAAGAASAMKRGQYT
                     LTGTTVVGNVATAFLREVNGGKSR"
BASE COUNT          915 a         1820 c         1802 g          880 t
ORIGIN      
        1 cgtcgtgctc ggcatcgtcg gcagcaacta tctcggcaag agcgagcagg cgaaggcaaa
       61 ggccgccaag atcgagatcg agcagatttc ccaggcgctc gacttgttca agctcgagct
      121 cgggcgctat ccgacgacgc aggaggggct gcaggcgctc gtcgcggccc cgccgggcct
      181 cgccaactgg gccggccctt actggaagaa gtcgacgaca ccgaaggacc cgtggtcgca
      241 cgaatacaag tacgcgtcgc cagggacgca cggcgcgtac gacatcattt cgcttggcgc
      301 cgatggtgtc gaaggcggtg agggcgcgaa caaggatgtg acgaattggg agtaagaacc
      361 ggcccatgca gtgacccgcg ggcgaaggga atgagcgtac gtcggtgctc tttcggcgtg
      421 acgctgctcg agcttttgat cgtcatcgcg ttgatggcaa tcgttgccgg cttcgtgctg
      481 ccgatgttcg gcggcccggt gtcgacgagc gagctccgcg caacggcgcg ccagctcgcc
      541 gccgggttgc gccttgcgcg aagcgaagcg cttgccgagc gtcgcgagac gttcctcgtc
      601 gtcgatgttg cgggcagacg attcaaggtc gaccgcgatc ctgccgagca caagctttcg
      661 tcgaagatcg acatcaagct cttcaccgcg cagaacgatc tcgtcgacaa caacatcggg
      721 tcgattcgat tctttcccga tggcggcagc aacggcggcc gcatcacggt tgcctctggc
      781 gagcgcaaat tcgacgtcga cgtcgactgg ctgacgggtc gcgtcgcgat cctcgattga
      841 catgagtgac gcgcgtcgca atcctcatcg cgggttctcg ctgctcgaag tgctcgtcgc
      901 cttcgtcatc gtcgcgctcg tggtcaccgc gttgtttcgc ctcttcggcg gcgcgcttgg
      961 caacgcgtcg gcggccgatg aatggacgcg tgcgctgctc gtcgcgcaga gccggctcgc
     1021 cctggcggcg tcggcgcagc cgctgcgcga gacgaccgat gcagggagcg aggccgacgg
     1081 tcgtatcgat tggcgaacgt cggtgactcc gtatgtcccg cctgatggcg atcccgacgt
     1141 cgagcgcgca tccgaaacga tggcgacgcg actgtttcgc gtcactgtcg acgtgcgctt
     1201 tccaggaatc gcgggccaga cgcgaacgct gacgctgtcg acggtgaagc tcggacaaag
     1261 gaacccagca tgaggacagc ggttcgaggc atgaggacgg cgcgcaccgt gcgcggcttc
     1321 acgctgctcg aattgatgat cgcgctgacg ctgctggcgc tgctatcggc cgttctgttc
     1381 gggtcattgc gtctggcggg acgcagcacc gatggcggcg acgcgaaagt ggaagcggcg
     1441 gcatcgatgc ggcttgcgca ggaattcctg cggacgaacc tcgaagcgca acatccactg
     1501 cggatgcgga agatcgtcga ttcaccgctt ctcttcagcg gcacgcgcaa cgaattgcgc
     1561 tatgccgccg agttgccgcc gcgcgtcgcc ggaggcggca tctggttcta tcggttgtcg
     1621 cttcgcaccg aagactcccg cgggccattg acgctcgagc gcgtcgtccc cgacctcgcc
     1681 gccgatgcga tacccgagtt cgcgaacgcc gaccggtcgg tgctggccga cggcatcgcg
     1741 gaattgacgc tcgggtattt cggtcgcgat accgacgcgg cgacgaccgt tgcgcccacg
     1801 tggcgcgatc actgggacaa cgtgcagcag ctgccgatga tgatccgcat cgacgtcaaa
     1861 ccaaagcaag gtccggcatg gccgaccctt tatgtgacgc cgcgggaggc acccgaggct
     1921 gggtgccgtg cgtgggacat cgcgcgtcag cgatgcgcag cggtttgatc acgaaccgcc
     1981 gaccacttcg ttcgcgaacg cgcaatgcgc agtcgggcat cgcgctcgtg ctcgcactgt
     2041 ggcttaccgt gttgttgacg gtgctcgcga gtggcttcgc gttcagcatg cgcaccgaag
     2101 cgctcgcggt gcgcaacgcg gtgtcgcttg cacaggcgcg cgtgatcgcc gacggcgccg
     2161 tcgagcgcac ggcgtacgag ctgatgcgtc cgcagatcgc gaacgtctgg aagcccgacg
     2221 ggcagccgca ccggttcgtc gacggcgaag ccaccgtcac cgtcacggcc gtcgacgaag
     2281 cggcgaagat cgacatgaat tcggcgcccg atccgttgct gaagaacctg ctgatcgtca
     2341 tcggcggact cgacgaagca acggcaggca acctcgtcga cgctatcgac gactggcgcg
     2401 accccgacga tctgcgccgt cccaatggcg cagagttacc ggaatatcgc gccgccaatt
     2461 tgaagtacgg ccccgcgaat gcaccattcg agacgatcgg cgaagcggcg cgcgtgctcg
     2521 gcatgacggc cgacgtgtac aaccggattg caccggccat caccgtttat tcgcaccagc
     2581 ccggcgtcaa tccggcgacg gcgtcgcgca gcgtgctgct cgcgctgccg aatgcgacgg
     2641 cggataccgt cgacgcattc atcgcgcagc gtgcgcaggc gctggccgac aagctgccgg
     2701 tgcctccatt ccccccggcg caggcattcg gcagcgggcc ggtgcagatc tggcgtatcc
     2761 gcgccgaggc gacgctgccc gatggtgtaa cctttgcccg cgaagctgtg ctgcgcccgt
     2821 cgcccgacgc gcagcggccg ctgattgcgc tcgcatggct cgagccaacc cgatcagttg
     2881 ccgcgccttc cctttctgaa cccactgctg atggccgccg tcgctgactc gacatcgttc
     2941 aacaagcccg gatacgcacg ggacgtcgca cgccggctcg ggctgaccgg gttcggtcac
     3001 tggtgggcgc gcgagcttgc cgcgctgatg ccgtcgcgat tgcgcgccgc cgttgcgcat
     3061 cggcacgcgc gaccgctgct cgcgttcgac ggcgcgcagg caacgctgtg gcgcccggtg
     3121 acctccgccg ggcaggtgac gatggtcgaa gcggcgcgca ttccactcga cggcgaccct
     3181 gcgacagtcg cggcgggtgg acgcgcagcg ctcgcgccgc tgactcgcgg tgcgaatgga
     3241 ggagcgccgg aagtcgtcat tgcattgtcg ccgcgcgcgt cattgcgcaa acggttgacg
     3301 ttgccggcgg caatcgagcc gaacttgcat caggcgctcg cctacgatct cgatcggcac
     3361 acgccattca aatcgggcga gctctatttc gacgccgcga tcgccgatcg cgatcctgca
     3421 aacaacacat tgcatgtcga tctcgcggcc gctcgccgcg cgatcgtcga tccgatgctg
     3481 cgccatgcag agagtttcgg cgcacgcgtc gtcggcatca ccgtcgatcc gccggcgatt
     3541 gcatcgactt cgcggctcga tctgcttccc gtcgaacgcg aggatcgcac cgagcgctcg
     3601 cgctggcggg tgatcgttcc actggtgctg ctcgcgattg cgattctcat agcactcgtg
     3661 cttcccgtct ggcagaagcg cgaggaggcg attgcgctca accagctgtc ggaccaggcg
     3721 cggcagcgcg ccggcgcttc ggacgcgctt cgcaccgagc tcgagcggaa gacgagcgaa
     3781 tacaacttcg cgctcgagcg caagtacgcg ttcccgggaa ccgtgcaggt gctcgacgac
     3841 gtcacgcatc tcctgcccga cgacacgtgg ctcacgcagc tcgaagtgcg cagcgttcgc
     3901 ggcaaggagg gacagcgcga gctcacgcta cggggggaat cggcgaatgc cggtcggctc
     3961 gtgtcgctgc tcgaggattc gaagctcttc acccaagcgg ccccgcgttc gcccacgacc
     4021 aagatccagc cgggccccgg cgagattttc gatgtcggcg ctcagctgaa acccgccgcc
     4081 atgccgcagc cggcgccact tgatttgacg gcaccaccgc cgcaagcgcc gcagcgagcg
     4141 cgcagcaatc cggccacgca gccttcgacg ccggcaaccg ccgcgacaca ggcgggtgca
     4201 gcggcccctg ccgccgcgcc cgcggcagca ccgaccaatc caccgcctgt cccgaatcgc
     4261 cgctccgtca tcacgcgcag tgcgccgccc gcgcctgctg cggcaccagc aggggccgca
     4321 ccgcagccgg cgcccggagc accaccggta actgcagagc cggagcccgc cgatggcgaa
     4381 ggagcaacgc aatgacgccg ccggcgctga aggggttaac cgctgcgcag cagcgcgcgc
     4441 ttgccgccgg gctgttgttc ggtggcattg cggcactgct cttgatcgtg ctcgttccga
     4501 tcgtgctcct gcatcggcat tacgacgcgg cgatcgccga cacgtcggac aagctgcagc
     4561 gctaccagcg aatcgctgcg caggcgcccg cctttcggac ggcgctcgat gcgatgcagc
     4621 agcgcgatgg ccgccgcttc ttcctgcgga acaccgcgtc gaacctcgcc ggcgccgagc
     4681 tgcaggagct ggtcaaggcg gcgatcgaag gtaacggtgg ccggatcacg acgagccaga
     4741 acacgagccc gcgggaggac gggcgctttc gcgagatcgg cgtcaacgtc cagttcttcg
     4801 cgacgacgcc ggcgctgcaa cgaatcctgc tcgcgatcga gacgcaccag ccgtacctga
     4861 tcgtcgagaa cctgacggcg cggccgctca acgcatttcg tggcttcaag ccggcacccg
     4921 gccaggagcc tgagctcaac gtgcaactcg acgtcggtgc gtgggctttt cccgagcctg
     4981 cgcggcagac ggcgccgccg aaagccgcgt catgaagagc gcgctgacga ggagaaacgc
     5041 atgaacgacg ccagtgcgct tgcgcggacg ttgatcgtct ggatcgttcc gtttgccatc
     5101 gctgtcgcgg caatcggcat cgagacggac tggggccgct cgctcaatcg cgatacgccg
     5161 gcaccgaccg ctgtaacgcc tcaaccggtg gcggttgcgt tgctccccga gtatcgaatc
     5221 gacggcggca ccgaagcgcg gcgcgagacg gtcgatcgcg tgctgttcaa cccgacccga
     5281 cgcccggcac cgcctgcgaa ccaggctgcc ggcgccgcat cggcgatgaa gcgaggccag
     5341 tacacgctga cgggaaccac ggtagtcggg aatgtcgcga cggctttttt gcgcgaggtg
     5401 aacggcggca agtcgcg
//
LOCUS       DASSVX010000340         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_381964, whole genome shotgun sequence.
ACCESSION   DASSVX010000340 DASSVX010000000
VERSION     DASSVX010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381964"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1264)
                     /locus_tag="VFO53_14640"
     CDS             complement(53..1264)
                     /locus_tag="VFO53_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET8877563.1"
                     /translation="MNDVSTRSGVRIVLPEAGGRLATHELREPRHFPQRAARAFTRVA
                     YSAAHVVSDPLAAVDPSLDVAIDWDATIAYRRHLWSLGMGVAEAMDTAQRGMGLDWPT
                     SLELIRRTLDAARDVPGALVASGAGTDHLALADARSLDDVIAAYEQQIEAIEALGGRI
                     VLMASRALARLARSPDDYVRVYAKVLAQVREPVILHWLGEMFDPALAGYWSGLRDAGA
                     DPGHATAMDVCAAVIADNASRIDGIKVSLLDAGKEIDLRGRLPAGVKMYTGDDFNYAE
                     LIAGDEQGYSDALLGIFDAIAPAASAALAELADGNIDAFHETLAPTVPLSRHIFAAPT
                     RFYKTGVVFMAWLNGHQSHFTMVGGQQSARSVVHLAELFRLADRAGLLSDPERAVSRM
                     RTLLALHGVDG"
     gene            complement(1295..2446)
                     /locus_tag="VFO53_14645"
     CDS             complement(1295..2446)
                     /locus_tag="VFO53_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8877564.1"
                     /translation="MAQHRLGIIMNGVTGRMGANQHLVRSIVAIRAQEGVPLRNGDRV
                     MPDPILIGRNADKLRALAQAHGIARYGTDLERALANRDDTVFFDAATTQMRPALLKRA
                     IAAGKHVYCEKPIAVDLADALDLARAAERAGVRNGAVQDKLFLPGLLKLRMLRDAGFF
                     GRMLCVRGEFGYWVFEGDLQPAQRPSWNYRAEEGGGIILDMMCHWRYVLDNLFGEVKS
                     VSCLGARHIPKRWDENGQPYEATADDASYATFELEGGIVATINSSWCVRVRRDDLVTF
                     QVDGTHGSAVAGLQRCLTQARVNTPRPIWNPDVRQPIDFHAGWDEVPDATFYDNGFKV
                     QWERFIRHVVEDAPFQWTLREAAKGVQLVELALQSWKERRFVDVPALPA"
BASE COUNT          444 a          903 c          871 g          414 t
ORIGIN      
        1 tcgacagcaa cccaggatcg ggagacgtca tggggtcgtg accgcgttcg cgtcagccgt
       61 ccacaccgtg cagcgcgagc agcgttcgca tgcgcgacac cgcgcgctcg ggatcggaca
      121 agaggcccgc gcgatcggcc agacgaaaga gctcggccag atgcacgacg ctgcgcgcac
      181 tctgctgtcc gccgaccatc gtgaagtgcg actggtgacc gttcagccac gccatgaaca
      241 ccacacccgt cttgtagaag cgcgtggggg cggcgaaaat gtgccgcgac agcggtacgg
      301 tcggcgcgag cgtctcgtgg aacgcatcga tgtttccgtc ggcgagctcc gccagcgcgg
      361 cggaagccgc cggcgcaatg gcgtcgaaga tgccgagcag cgcgtcggaa tagccctgct
      421 cgtcgccggc aatcagctcc gcatagttga agtcgtcgcc ggtgtacatc ttcacgccgg
      481 ccggcaggcg cccgcgcagg tcgatctcct tgcccgcgtc gagcagcgac accttgatgc
      541 cgtcgatcct cgacgcattg tccgcaatca ccgccgcgca gacgtccatc gcggtcgcat
      601 ggccgggatc ggcgccggcg tcgcgcagcc cgctccagta gccggcgagc gcaggatcga
      661 acatttcgcc gagccagtgc aggatgaccg gctcgcgcac ctgcgccagc actttcgcgt
      721 agacacgcac gtaatcgtcg ggcgagcgcg caagccgggc gagcgcgcga ctggccatca
      781 gcacgatgcg accgccgagc gcttcgatcg cttcgatctg ctgctcgtac gcggcgatca
      841 cgtcgtcgag cgaacgcgca tcggcgagtg cgaggtgatc ggttcccgca ccgctcgcga
      901 ccagcgcgcc cggcacgtcg cgtgccgcgt cgagcgttct gcggatcagc tcgagcgacg
      961 tcggccagtc gagtcccatg ccgcgctgcg cggtgtccat cgcctcggcc acgcccatgc
     1021 cgagcgacca tagatggcgg cgataggcga tcgtcgcgtc ccagtcgatc gcgacatcga
     1081 gcgacgggtc gacagccgcc agcggatcgg acacgacgtg cgccgcagaa taggcaacgc
     1141 gcgtgaatgc gcgcgccgca cgctgcggaa aatggcgcgg ctcgcgcagc tcgtgcgtcg
     1201 ccagtcggcc gccggcctcg ggcagcacga tccgaacgcc gctgcgggtg gaaacgtcgt
     1261 tcatcgtggc gctctttcgc tttgcattgc gcgctcaggc aggcagcgcc ggcacatcga
     1321 cgaagcgtcg ctccttccac gactgcaacg cgagctcgac gagctgcacg cccttcgccg
     1381 cttcgcgcag cgtccactgg aacggcgcgt cctcgacgac atggcggatg aaccgttccc
     1441 attgcacctt gaagccgttg tcgtagaacg tcgcgtcggg cacctcgtcc cagccggcat
     1501 ggaagtcgat cggctggcgc acgtcgggat tccagatcgg ccgcggcgtg ttgacgcgcg
     1561 cctgcgtgag acatcgctgg agtcccgcga ccgccgaacc atgcgtgccg tcgacctgga
     1621 acgtgaccag atcgtctcgg cgcacccgca cgcaccagct gctgttgatc gtcgcgacga
     1681 tgccgccttc gagctcgaac gtcgcgtacg acgcgtcgtc ggccgtcgcc tcgtagggct
     1741 ggccgttctc gtcccagcgc ttcggaatgt ggcgggcgcc aaggcagctc acgcttttca
     1801 cctcgccgaa cagattgtcg aggacgtagc gccaatgaca catcatgtcg aggatgatgc
     1861 cgccgccctc ctcggcgcgg taattccacg acgggcgctg cgccggctgc aggtcgccct
     1921 cgaacaccca gtagccgaat tcgccgcgca cgcagagcat gcgaccgaag aaacccgcgt
     1981 cgcgcagcat ccgcagcttg agaagcccgg gaaggaacag cttgtcctgc acggcaccgt
     2041 tccggacacc ggcgcgctcg gcggcacgcg cgagatcgag cgcgtcggcg aggtcgaccg
     2101 cgatcggttt ttcgcagtag acgtgcttgc cggcggcgat ggcacgcttc agcaatgcgg
     2161 gccgcatctg cgtcgttgcc gcatcgaaga acacggtgtc gtcgcggttc gcgagcgcgc
     2221 gctcgagatc ggtgccgtag cgcgcgatgc cgtgtgcctg cgcgagcgcg cgcagcttgt
     2281 cggcgttgcg tccgatcagg atcggatcgg gcatcacgcg gtcgccgttg cgaagcggca
     2341 cgccttcctg cgcgcgtatc gcgacgatcg agcgcaccag gtgctggttc gcgcccatcc
     2401 gtccggtgac gccgttcatg atgatgccga gacgatgctg ggccatgggt aacctcggtt
     2461 tgccgatatt tttctacttc agcagcaggc tgggaagcca gagcgacagc gccggcagat
     2521 atgtgacaag cagcagcaca accagcattg gcacgtagaa gatcaggatc gagcgcgaca
     2581 ccgcctccat gctgacccgt ccgatcgcgc agccgaccac catggtcggg cc
//
LOCUS       DASSVX010000341         4513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1415247, whole genome shotgun sequence.
ACCESSION   DASSVX010000341 DASSVX010000000
VERSION     DASSVX010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415247"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFO53_14650"
     CDS             <1..180
                     /locus_tag="VFO53_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HET8877565.1"
                     /translation="LLGTVFNFNSLFVNLPCAWLASRAGRRWRGQADALHWMRRIVGG
                     VFVLLAVRLAALSRD"
     gene            213..1820
                     /locus_tag="VFO53_14655"
     CDS             213..1820
                     /locus_tag="VFO53_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET8877566.1"
                     /translation="MPIVESKLNPRDPTFARNRDAMAALVADLREKIASIEQGGGDAS
                     RARHVARGKLLPRERVRALIDAGSPFLELSQFAAYGMYDDNIAGAGLITGIGRVSGRE
                     CVIVCNDATVKGGTYFPMTVKKHLRAQEIARQNGLPCIYLVDSGGANLPTHTDVFPDK
                     EHFGRIFYNQATLSAAGIAQIAVVMGSCTAGGAYVPAMCDESIIVRNQGTIFLAGPPL
                     VKAATGEVVTAEELGGADVHTRISGVADHYAQNDHHALSIARRIVGNLNTRKPAELDV
                     TEPIAPRYAADELYGVINADIRKPYDVREVIARIVDDSNLDEFKARYGATLVTGFARI
                     WGYPVGVVANNGVLYSESALKGAHFIELCVQRGIPLVFLQNITGFMVGKKYEAGGIAR
                     DGAKMVTAVSCAQVPKFTVIIGGSYGAGNYGMCGRAFNPRFLWMWPNARISVMGGEQA
                     ATVLATVRRDGVEARGESWSVEDEEAFKTRIRTQYEREGHPYFASARLWDDGVIDPVD
                     TRRTLALAISASLNQPIESTRFGVFRM"
     gene            1817..2959
                     /locus_tag="VFO53_14660"
     CDS             1817..2959
                     /locus_tag="VFO53_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8877567.1"
                     /translation="MITVHDTPERTLLRDQVARFLAHDVEPYGDAWERAGSVPRDVLR
                     RMAELGWLGLMVPQAYGGADVDALTNFAFAEALSQSSYGGFIVTVLVHTDMASPHLVN
                     AGTDAQKACYLPGIVSGATLTAVAITEAGAGSDVAGIRTTARRDGDAFVLDGSKLFIT
                     NGVSADVYFVAARTAPLDTRSRGVSMFIVEKNTPGFRVGQVLDKAGWRSSDTAELVFD
                     NCRIPASSLLGEENYGFRALMQNFQRERLALAAMAIGNADRALATALEHVRERRAFGG
                     VLWDREAIRQKLASLDARRQAVRAFAWHCAWLVAQRHDAVREISQLKALAGELANDVA
                     YGCQQCFGGMGYIRDTSIERLWRDARILSIGGGATEVMLEEAAKRL"
     gene            2956..3792
                     /locus_tag="VFO53_14665"
     CDS             2956..3792
                     /locus_tag="VFO53_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8877568.1"
                     /translation="MTPTDPAPPQSNDAADNPCELAIRDGIAMVTLNRPAIHNAFDER
                     LIAKLTQTFVELDDERDVRVVVLAGAGRSFCAGADLNWMRRMAAFDHDENLRDATALA
                     AMLRALYALSKPTIARVHGAAYGGGVGLIAACDIAIAVPEATFALTEAKLGLIPATIG
                     PYVIEAIGARQARRYFLTAERFAAAEALRIGLVHEVVDSSQLDARIDGIIGALRAAGP
                     FAQLECKALIRGVAHRPIDADVISGTAGHIAAVRASPEGKEGVAAFLDKRTPAWIESS
                     KQ"
     gene            3801..4442
                     /locus_tag="VFO53_14670"
     CDS             3801..4442
                     /locus_tag="VFO53_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HET8877569.1"
                     /translation="MASLAHFDTWQLVALAASLGIASGLRLYAVLFIVGAAGFLGWVD
                     LPSGLAALAHPLVLAASGFMCFVEFFADKIPGVDSLWDLVHTLIRIPAGAALAASVFG
                     DSSSATMLAAAILGGTLAAGSHFAKAGSRALINTSPEPFSNWGASLTEDVAVGGLLWL
                     AIAHPLAAAVVIGLLILFAIWMLPKLWRTGRRVAGRLSGALTSSPAKSGAARR"
BASE COUNT          732 a         1451 c         1536 g          794 t
ORIGIN      
        1 ctgctcggca cggtgttcaa cttcaacagc ctgttcgtga atcttccctg cgcatggctc
       61 gcgagccgcg ccggacgccg gtggcgcggt caggcggatg cgctgcactg gatgcggcgc
      121 atcgtcggcg gcgtgttcgt attgcttgcc gtgaggctcg cggcgctgtc gcgcgactga
      181 cgctcgccgt tcgcctcacc aatggagccg cgatgcccat cgtcgaatcg aagctgaatc
      241 cgcgcgatcc gacgttcgcg cgcaaccgcg acgcgatggc ggcgctcgtc gcggatttgc
      301 gggagaaaat cgcgtcgatc gaacaggggg gtggcgatgc gtcccgcgcg cggcacgttg
      361 cgcgtggcaa gctgctgccg cgcgagcgcg tgcgcgcgct gatcgatgcg ggcagcccgt
      421 ttctcgagct gtcgcagttc gccgcctacg gcatgtacga cgacaacatc gccggcgcgg
      481 gtctcatcac cggcatcggt cgcgtgtccg ggcgcgaatg cgtgatcgtc tgcaacgacg
      541 cgacggtcaa aggcggcacg tattttccga tgacggtgaa aaagcatctg cgcgcgcagg
      601 agatcgcgcg gcagaacggc ctgccgtgca tctatctcgt cgattcgggc ggcgcgaacc
      661 tgccgacgca cacggacgtg tttcccgaca aggagcattt cgggcgaatc ttctacaacc
      721 aggcgacgct gtcggctgca ggcatcgcgc agatcgccgt cgtcatgggc tcgtgcacgg
      781 cgggcggcgc gtacgtgccg gcgatgtgcg acgaatcgat catcgttcgc aaccagggaa
      841 cgatctttct cgccggaccg ccgctcgtga aagccgcgac cggcgaggtc gtcaccgccg
      901 aagagctcgg tggcgccgac gtccatacgc ggatatcggg ggttgccgac cattacgcgc
      961 agaacgatca ccatgcgctc tcgatcgcgc ggcggatcgt cggcaacctg aacacgcgca
     1021 agccggctga gctggacgtg accgagccca tcgcgccgcg gtacgcggcc gatgaacttt
     1081 atggcgtcat caacgcggat atccgcaagc cgtacgacgt tcgcgaagtg atcgcgcgca
     1141 tcgtcgacga ttcgaacctc gacgagttca aggcgcgcta cggagcgacg ctcgtcactg
     1201 gcttcgcgcg aatctggggt tatccggtcg gcgtcgtcgc gaacaacggc gttctctatt
     1261 cggaatcggc gttgaagggc gcgcatttca tcgagctttg cgtgcagcgc ggcatcccgc
     1321 tcgtatttct gcagaacatt accggcttca tggtcggcaa gaaatacgag gcgggtggca
     1381 tcgcgcgcga cggcgcgaaa atggtcacgg ccgtatcgtg tgcgcaggtg ccgaagttca
     1441 ccgtgatcat cggcggcagc tacggcgccg gcaactacgg gatgtgcgga cgtgcgttca
     1501 atccacgctt cttgtggatg tggccgaatg cgcggatttc ggtgatgggg ggcgagcagg
     1561 cggccacggt gctcgcgact gttcgccgcg acggtgtcga ggcgcgcggg gagtcgtgga
     1621 gcgtcgaaga cgaggaagca ttcaagacgc ggattcgcac gcaatacgag cgcgaaggcc
     1681 atccgtattt tgcaagcgct cggctgtggg acgatggtgt catcgatccc gtcgacacgc
     1741 ggcgcacgct ggcgctcgca atttccgcat cgttgaacca gccgatcgaa tcgacgcgat
     1801 ttggcgtgtt tcgcatgtga tcaccgttca cgacacgccg gagcggacgc tcttgcgcga
     1861 ccaggtcgcg cgcttcctcg cgcacgatgt cgagccgtac ggcgatgcgt gggagcgcgc
     1921 aggcagcgtg ccgcgcgacg tgctgcggcg aatggccgag ctcggctggc tcggcttgat
     1981 ggtgccgcag gcatacggcg gcgccgacgt agacgcgctc accaattttg cgtttgccga
     2041 agcgctgtcg caatcgagct acggcggctt catcgtcacc gtgctcgttc acaccgacat
     2101 ggcgtcaccg catctcgtca acgccggtac cgacgcgcag aaggcgtgct acctgccggg
     2161 gatcgtcagt ggcgcgacac tgaccgcggt cgccatcacc gaggccggcg ccggctccga
     2221 cgtcgcaggc atccgcacga cagcgcggcg cgacggcgat gcgttcgtgc tcgacggcag
     2281 caagctcttc atcaccaatg gtgtcagcgc cgacgtctat ttcgtcgccg cgcgcaccgc
     2341 accactcgac acgcgctcgc gcggtgtgtc gatgttcatc gtcgagaaaa acacaccagg
     2401 ctttcgcgtc ggacaagttc tggacaaggc cggctggcgc tcgtccgata cggccgagct
     2461 cgtattcgac aactgccgga taccggcaag cagtcttctt ggtgaggaaa actacggctt
     2521 tcgcgcgctg atgcagaact tccagcgcga gcggctggca ctggctgcga tggcgattgg
     2581 caatgcggac cgcgcactcg caacagcact cgagcacgtt cgggagcggc gcgcgttcgg
     2641 cggcgtgctg tgggatcgcg aagcaatccg gcaaaagctc gctagcctcg atgcgcggcg
     2701 ccaggcggtg cgcgcgttcg cgtggcactg cgcgtggctc gtcgcgcaac gtcatgacgc
     2761 ggtgcgcgag atctcgcagc tcaaggcgct cgcgggcgag ctcgccaacg acgtcgccta
     2821 tggctgccag cagtgctttg gcgggatggg ctatattcga gacacgtcaa tcgagcggct
     2881 gtggcgcgac gcacgcatcc tgtcgatcgg cggcggcgcc accgaagtga tgctcgagga
     2941 agcagccaaa cgcttatgac accgaccgat cccgcgcctc cgcaatcgaa cgacgccgca
     3001 gacaatccgt gcgagctcgc gatccgcgac ggcatcgcga tggtgacgct gaaccgtccc
     3061 gcaatccaca acgcgttcga tgaaaggctg atcgcgaagc tgacgcaaac gttcgtcgag
     3121 ctcgatgacg aacgcgacgt tcgtgtcgtc gtgctcgccg gcgcgggtcg aagcttctgt
     3181 gccggagccg acctcaactg gatgcggcgg atggctgcgt tcgaccacga cgagaatctg
     3241 cgtgacgcga cagcgctggc cgccatgctg cgcgcgctct atgcgttgtc gaaaccgacg
     3301 atcgcgcgcg tgcacggcgc ggcgtacggg ggcggcgtcg gcctgatcgc ggcctgcgac
     3361 atcgcgatcg cggttcccga ggcgaccttt gcgctgaccg aggcgaagct cggactcatt
     3421 cccgcgacga tcggccccta cgtgatcgag gcgatcggtg cgcggcaggc gcgccggtat
     3481 ttcctgactg ccgaacgttt cgcagccgcg gaggcgttac ggatcggtct cgtgcacgag
     3541 gtcgtcgatt cgtcgcagct cgatgcgcgc atcgacggca tcatcggcgc gttgcgcgcg
     3601 gcggggccgt ttgcgcaact cgaatgcaag gcgctgatcc gcggcgtggc gcaccggccg
     3661 atcgacgccg atgtcatcag cgggaccgcg ggccacattg ccgccgtgcg ggcatcaccg
     3721 gagggcaagg agggcgtggc agcattcctc gacaagcgaa ccccagcgtg gatcgaatcg
     3781 tcgaagcaat aggctcctaa atggcatccc tcgcgcattt cgacacgtgg cagctcgtcg
     3841 cactggccgc atcgcttggc atcgcgagcg ggctgcgcct ttacgccgtg ctgttcatcg
     3901 tcggcgcggc cggtttcctc ggctgggtcg atttgccgtc gggactggcg gcgcttgcgc
     3961 acccgctcgt gctggcggcg tcgggcttca tgtgcttcgt cgagttcttc gccgacaaga
     4021 tccccggcgt cgattcgctg tgggatctcg tgcacacgct gatccgcatc ccggccggcg
     4081 cggcgctggc ggcgagcgtt ttcggcgatt cgtcatccgc gacgatgctc gcggcggcga
     4141 tcctcggcgg cacgctcgcg gcgggcagcc atttcgccaa agccggcagc cgggcgctca
     4201 tcaacacctc gcccgagccc ttctcgaact ggggcgcgtc gctgaccgag gacgtggcag
     4261 tcggcggact gttatggctc gcgatcgcgc atccgctcgc cgcagccgtc gtgatcgggt
     4321 tgcttatcct tttcgcgatc tggatgctgc cgaagctgtg gcgcaccggg cgccgcgttg
     4381 ccgggcggct ctccggtgcg ttgacctcat caccggcgaa atccggcgcg gcgcgccgat
     4441 gaccgggatc cgatgttcgg cacgatcctg atcgccaacc gcggtgagat cgcgtgccgc
     4501 gtcgcgcgca cgg
//
LOCUS       DASSVX010000342         6869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_748571, whole genome shotgun sequence.
ACCESSION   DASSVX010000342 DASSVX010000000
VERSION     DASSVX010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748571"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFO53_14675"
     CDS             complement(<1..444)
                     /locus_tag="VFO53_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HET8877570.1"
                     /translation="MVPSASSSSTPLSYRDAGVDIDAGDALVERIKPFARRTMRPEVL
                     AGIGGFGALIEIGKRFREPVLVAGTDGVGTKLDLAQRLGRHDTIGIDLVAMSVNDILV
                     QGAEPLFFLDYFVCERLDVDVAADVIEGIAKGCELAGCALIGGETA"
     gene            710..1786
                     /locus_tag="VFO53_14680"
     CDS             710..1786
                     /locus_tag="VFO53_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET8877571.1"
                     /translation="MVTPRRHPLAPKQYLWVVGALAVLFVALTWLGPVLTPFLIGAIL
                     AYLGTPAVARAERRGVPRSLATLFVILFIGLLLAALFLVLIPLVQSEVATITRRLPDL
                     FAQFTDRVGPFLQEKFDVELSLDFDSIRTFISENAQEARNLSARLLSGVKTGGAIVLS
                     LLVNLALVPVVMFYLLRDWNMILARIDDLLPRRWGPKVRSIGREVDGVLAEFLHGQLL
                     VMLALAAYYSIALSLVRLDHAAAIGILTGLLVFIPYVGFGLGFLLGMTAAILDFHGWP
                     GFFAVLVVYGVGQLLESYVLVPFLVGDRIGLHPLAVIFALLAFGELFGFAGVLMALPV
                     SAALLVGLRHLRRVYVDSPVYREE"
     gene            1812..2504
                     /gene="hda"
                     /locus_tag="VFO53_14685"
     CDS             1812..2504
                     /gene="hda"
                     /locus_tag="VFO53_14685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HET8877572.1"
                     /translation="MNEQLILDLAQPEPATFANFVAGANREALAALAALASGASREAG
                     LLLWGAHGAGKSHLLDACAGAARIAMRPVVRCASPRELPGDDDIAPSTLLVVDAVDSA
                     DAHAQGRLFTLINGLAAVQGQWIAAAALPPARLALREDLRTRLALGLVLELLPLADAD
                     KPQALAAYARERGFHLADDVIEYLLAHGRRDMTSLVAALAALDRRSLAIKRSVTVPLL
                     REWLQGDLGLPQ"
     gene            2689..3027
                     /locus_tag="VFO53_14690"
     CDS             2689..3027
                     /locus_tag="VFO53_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877573.1"
                     /translation="MNKLLAMLVAGAFVATSTIALAQTPATPATPATPATPATPPAAS
                     SGSMKADSMDHGSMTKKSSRKAKKAAKKKAAVAKEDQMIKNSTHSSNSAVKAAPATGA
                     AATKADTKGK"
     gene            3148..3654
                     /locus_tag="VFO53_14695"
     CDS             3148..3654
                     /locus_tag="VFO53_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET8877574.1"
                     /translation="MNRAVQTHSAPVESANDAVGPFSPASRPVITVATIAERDGRFLM
                     IEEETRVGIRLNQPAGHVERGESIAAAAARETLEEAAWRVDPTALVGVYHWQSPLYGT
                     YVRFTFIALPRAHEPARALDTGIVRTLWLTYDEIAAHPMHRSPFVLRSLDDYRAGRRF
                     PLGLIASL"
     gene            3657..4781
                     /gene="mnmA"
                     /locus_tag="VFO53_14700"
     CDS             3657..4781
                     /gene="mnmA"
                     /locus_tag="VFO53_14700"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET8877575.1"
                     /translation="MRGKRVVVGMSGGVDSSVAAWLLKRQGFDVVGVFMKNWEDDDTD
                     EYCTSREDLVDAASVADVIGVELDAVNFAAEYRERVFEHFLRDYAAGRTPNPDVLCNS
                     EIKFRAFVDYAQVLGADAIATGHYARVAVGADGRVELLKAADASKDQTYFLHRLSQAQ
                     LAPVLFPLGGMTKRDVRAIARRERIPTYAKRDSTGICFIGERPFRDFLARYLPRTPGP
                     IVTADGTTIGEHQGLAYYTIGQRQGLGVGGVRDGAQAPWFVAGKDMGRNALIAVQGHG
                     HPLLCRRDVRAVDMHWIGGDAPALPARLTAKTRYRMPDATCVVNVCGEDIVAAFDAPQ
                     WAPTPGQYLVLYDGQRCLGGGVIDAPLSMAEVEAASLLEE"
     gene            complement(4767..5318)
                     /locus_tag="VFO53_14705"
     CDS             complement(4767..5318)
                     /locus_tag="VFO53_14705"
                     /inference="COORDINATES: protein motif:HMM:NF019415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HET8877576.1"
                     /translation="MAAASHLDTLDYAKKLEAAGVPKAQAELQASALNDALAGSVVSR
                     SDLSNLAQGIDGEFASLQAKIDARFQQVDARFDNVDARFDNVDARFDARFDNVDARFD
                     AMLANVDSRFHNVDARFDAMLANVDARFDNVDLKLAHLESRVDARIGSVELKLGGKIE
                     TLQWMLGVIVAMNAAVLVQLFLK"
     gene            complement(5373..5858)
                     /gene="ssb"
                     /locus_tag="VFO53_14710"
     CDS             complement(5373..5858)
                     /gene="ssb"
                     /locus_tag="VFO53_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET8877577.1"
                     /translation="MASVNKVILLGNLGRDPETRYTTGGDAVTNLNIATSEQWKDKSG
                     EKQERTEWHRVVLFGRQAEIAGEYLKKGRSVYIEGRLQTRKYTDKDGVEKYSTEIVGD
                     RMQLIGGSREGGGGGDVEFSGGGGAPSRREPAGAGAAGAGGKGGAAKKNVDDFDDDIP
                     F"
     gene            6071..>6869
                     /locus_tag="VFO53_14715"
     CDS             6071..>6869
                     /locus_tag="VFO53_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET8877578.1"
                     /translation="MDHIRIRGARTHNLKNLSLDLPRHRLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAISIEQKATSHNPRSTVGTVT
                     EIHDYLRLLFARVGDPYCINHPEQKLSAMTISQMVDATLAMPEDTRLMILAPVVVNRK
                     GEQVDLFAELRAKGFVRVRVDGTVYEIDAVPKLAKNTKHTVDVVVDRLKVRPEFKQRL
                     AESYETALRHGDSRAVAVEMDGGREHLYSAKFACPICNYSLSELEPRL"
BASE COUNT         1039 a         2302 c         2290 g         1238 t
ORIGIN      
        1 ggcggtttcg ccgccgatca gcgcgcagcc cgcgagttcg cagcccttcg cgatcccttc
       61 gatcacgtcg gcggcaacgt cgacgtcgag gcgctcgcag acgaagtagt cgaggaagaa
      121 cagcggctcg gcaccctgca cgaggatgtc gttgacgctc atcgcaacga gatcgatgcc
      181 gatcgtgtcg tggcggccga gcctttgcgc gaggtcgagc ttggtgccga cgccgtcggt
      241 gcccgcgacc agcacgggct cgcggaaccg cttgccaatt tcgatcagcg caccgaagcc
      301 gccgatgccg gcgagcacct ccggccgcat cgtgcggcgc gcgaacggct tgatgcgctc
      361 gaccagcgca tcgcccgcgt cgatgtcgac gccggcgtcg cggtacgaga ggggcgtgct
      421 ggaggacgag gcggacggca cgatgaggta aagtgcagcg tttttcgctc cgcgattcta
      481 cgccaccacc ctgcccgacc atgagcgccg ccgagccgcc tcaaggcgcg aattccgtcc
      541 cccggggggc agcgcagcgg cgtagccgca agcgtggggg gattctttga ccgctgcccg
      601 tccgccggtc gatgccggct ccgacaccga agcaggatcg gagacgacat tccgttcgcg
      661 cggcccgctt ccggacaacg agccgaaagt cgtctctgac cccacttcgg tggtgactcc
      721 gcgccgccat ccgctcgcgc cgaagcagta cctgtgggtc gtcggcgcgc tcgccgtgct
      781 tttcgtggca ctgacgtggc ttgggccggt gctcacgccg tttctcatcg gggccattct
      841 cgcctacctc ggcacgccgg ccgttgcgcg cgcggaaagg cgcggcgtgc cgcgctcgct
      901 cgccacgctg ttcgtcatcc tgttcatcgg tcttctgctc gccgcgctct tcctcgtgct
      961 gattccgctc gtgcaaagcg aagtcgcgac gatcacgcgc cggctgcccg atctgttcgc
     1021 gcaattcacc gaccgcgtcg ggccgttcct gcaggagaag ttcgacgtcg agctttcgct
     1081 cgacttcgat tcgattcgca cgttcatctc ggaaaacgcg caggaggcgc gcaacctgtc
     1141 ggcgcgtctt ctgtcgggcg tgaagacggg cggcgccatc gtgctgtcgc tgctcgtcaa
     1201 tctcgcgctc gtcccggtcg tgatgtttta cctgctgcgc gactggaaca tgattctcgc
     1261 gcgcatcgac gacctgctgc cgcgccgctg gggcccgaag gtgcgcagca tcggccgcga
     1321 ggtcgacggc gtgctcgccg aatttctgca cgggcagctg ctggtgatgc tcgcactcgc
     1381 cgcgtattac tcgatcgcgc tttcgctggt gcggctcgat catgccgccg cgatcggcat
     1441 cctgaccggg ttgctggtct tcattccgta cgtggggttc gggctcggct tcctgcttgg
     1501 catgaccgcg gcgatactcg actttcacgg ctggccgggc tttttcgcgg tgcttgtcgt
     1561 gtacggcgtc gggcagttgc tcgaaagcta cgtgctggtg ccatttctcg tcggcgatcg
     1621 aatcggcctg catccgctcg cggtgatctt cgcgctgctc gcgttcggcg aattgttcgg
     1681 ctttgccggc gtgctgatgg cgctgccggt gtcggcggcg ctgctcgtcg ggttgcggca
     1741 tctgcgtcgt gtctacgtcg attcgccggt ttaccgcgag gaatgacgtg ttccgctgat
     1801 cgcgtccgcg gatgaatgag cagctgattc tcgatctcgc gcagcccgag ccggcgacgt
     1861 tcgccaactt tgtcgcgggc gccaatcgcg aggcgctggc ggcgctggcg gcgctcgcgt
     1921 cgggcgcgtc acgcgaagcg gggcttctgc tgtggggcgc gcacggtgcg ggcaagtcgc
     1981 atctgctcga cgcgtgcgcc ggcgcggcgc gcatcgccat gcgcccggtg gttcgatgcg
     2041 cttcgccgcg cgagcttccc ggcgacgacg acatcgcgcc gtcgacgctt ctcgtcgtcg
     2101 atgccgtcga cagtgccgat gcccacgcgc agggacgcct gttcacgctg atcaacgggc
     2161 ttgcggcggt gcagggccag tggattgccg cggcggcgct gccgccggcg cgccttgcgc
     2221 tgcgcgagga tctgcgcacg cggctcgcgc tcggcctcgt gctcgagctt ttgccgctcg
     2281 ccgacgcgga caagccgcag gcgcttgcag cctatgcgcg cgaacgcggg tttcacctcg
     2341 ccgacgatgt gatcgagtac ctgctggcgc acgggcgacg cgacatgacg agcctcgtgg
     2401 cggcgctcgc ggcactcgac cggcgctcgc ttgcgatcaa gcgcagcgtc acggtgccgc
     2461 tgttgcgcga atggctgcag ggcgacctcg gcctgccgca atgactttcg cagcgcctgt
     2521 cgccagccgg cgacgggtgg cgcgtcgaat cgatgcgaca cgccgtgtgc cgcacgagtg
     2581 cggtgcacag ttgtcaacgc cggcaagcgg ggcggcgtta atggcggcga cgcgccgttg
     2641 cggcgtcaca actccaacat gaatcacttc aacttctcag gacccaccat gaacaagctg
     2701 cttgcaatgc tcgtcgccgg cgccttcgtc gccacgtcga cgatcgccct cgcgcaaaca
     2761 ccggcgacgc ccgcgacgcc cgccacgccg gcaacacccg ccacgccgcc ggcggcctcg
     2821 tcgggctcga tgaaggccga ttcgatggac cacggcagca tgacgaagaa gtcatcccgc
     2881 aaggcgaaga aggcggcaaa gaagaaggcc gcggtggcca aggaggacca gatgatcaag
     2941 aacagcacgc actcgtcgaa ctcggccgtg aaggccgcgc cggcgacggg cgctgccgcc
     3001 accaaagcgg acaccaaggg caagtgaacg cagccgtttc cggcaaaggc aggctgcggc
     3061 ctgccttttt tgtccacgcc gggctcgcaa cttgcagaga agtatcatcg gctttccaca
     3121 gaaaggcagg cttccgcctg cgacgcgatg aaccgagccg ttcagaccca tagcgctccc
     3181 gtcgaatcgg cgaacgatgc cgtggggccg ttttcgccgg catcgcgacc ggtgatcacg
     3241 gtggcgacga tcgccgagcg cgacggccgc ttcctgatga tcgaggagga gacgcgcgtc
     3301 ggcatccggc tcaaccagcc ggccggccac gtcgagcggg gcgaatcgat cgcggcagcc
     3361 gccgcgaggg agacgctcga ggaagcggcg tggcgggtgg acccgacggc gctcgtcggt
     3421 gtctatcact ggcagtcgcc gctttacggc acctatgtcc gcttcacgtt catcgcgctg
     3481 ccgcgcgcac acgagcccgc gcgggcgctc gacacgggca tcgtgcgcac gctatggctc
     3541 acgtatgacg aaatcgccgc ccatcccatg caccgcagcc cgttcgtgct gcgctcgctc
     3601 gacgattacc gcgccggccg gcgctttccg ctcggcctca tcgccagcct ttgacgatgc
     3661 gcggtaagcg cgtcgtcgtc ggcatgtccg gcggcgtcga ttcttccgtc gccgcgtggc
     3721 tgctgaaacg gcagggcttc gacgtcgtcg gcgtgttcat gaagaactgg gaagacgacg
     3781 acaccgacga atactgcacg tcgcgcgagg acctggtcga cgcggcatcc gtcgccgacg
     3841 tgatcggcgt cgagctcgac gcggtcaatt tcgccgccga ataccgcgag cgcgtgttcg
     3901 agcatttcct ccgtgactac gccgccggcc gcaccccgaa ccccgacgtg ctgtgcaaca
     3961 gcgagatcaa gttccgcgcg tttgtcgatt acgcgcaggt gctgggcgcc gatgcaatcg
     4021 caacggggca ttacgcgcgt gtggcggttg gtgcggacgg acgcgtcgag ctgctgaagg
     4081 cggccgacgc gtcgaaggac cagacgtatt ttctgcaccg gctgtcgcag gcgcagctcg
     4141 cccccgtgct gtttccgctg ggcggcatga ccaagcgcga cgtgcgtgcg atcgccaggc
     4201 gcgaacgcat tccaacctat gcgaagcgcg attcgaccgg catctgcttc atcggcgagc
     4261 gcccgttccg cgacttcctc gcacgctacc tgccgcgcac gccggggccg atcgtgacag
     4321 cggacggcac gacgatcggc gagcatcagg gactcgcgta ctacacgata ggacagcgcc
     4381 agggtctcgg cgtcggcggg gtgcgcgacg gcgcgcaggc gccgtggttc gtcgcgggca
     4441 aggacatggg ccgcaacgcg ctgattgccg tgcagggaca cgggcacccg cttttgtgcc
     4501 gacgcgacgt gcgggcggtg gacatgcact ggatcggcgg cgatgcaccc gcgctgccgg
     4561 ctcggctgac ggccaagacg cgctaccgga tgcccgatgc gacgtgcgtt gtaaatgtgt
     4621 gcggtgagga catcgttgcg gcgttcgacg cgccgcagtg ggcgccgaca ccggggcagt
     4681 atctggttct ctacgacggc cagcggtgcc tcggcggcgg ggtgatcgac gcgccgttgt
     4741 cgatggcaga ggtcgaggca gcgagcctac ttgaggaata gctggacgag cacggcggcg
     4801 ttcatcgcga cgatgacgcc gagcatccac tgcagcgttt cgatcttgcc gccgagcttg
     4861 agctccaccg agccgattct cgcgtcgacg cgtgactcca ggtgcgcaag cttgagatcg
     4921 acgttgtcga accgggcatc gacgttggcg agcatggcgt cgaacctggc gtcgacgttg
     4981 tggaagcggc tgtcgacgtt ggcgagcatg gcgtcgaacc ttgcatcgac gttgtcgaag
     5041 cgggcgtcga accttgcatc gacgttgtcg aaccttgcat cgacgttgtc gaacctggcg
     5101 tcgacttgct ggaaccttgc atcgatcttc gcctgaaggc tcgcgaactc gccgtcgatg
     5161 ccctgcgcga gattcgacag gtcgctgcgt gatacgaccg agcccgccaa cgcatcgttc
     5221 aatgcgcttg cctgcagttc ggcctgcgcc ttgggtaccc cggccgcctc gagcttcttc
     5281 gcgtagtcga gcgtgtcgag atgagaagcg gcagccatcg tcggctttcc gtcagtcagc
     5341 gcgaagacga cttcatcctc gaccgcaggc cgtcagaacg gaatgtcgtc gtcgaagtcg
     5401 tcgacgtttt tcttcgccgc gccacccttg ccgcccgcgc cggcggcccc ggcgcccgcc
     5461 ggttcacgcc gcgacggcgc gccgccgccg ccgctgaact cgacgtcgcc gccaccgccg
     5521 ccctcgcggc tgccgccgat cagctgcatg cggtcgccga cgatttcggt ggagtacttc
     5581 tcgacgccgt ccttgtcggt gtacttgcgc gtctgcaacc ggccctcgat gtagaccgag
     5641 cgcccctttt tcaggtattc gccggcgatt tcggcctggc gtccgaagag cacgacgcgg
     5701 tgccactcgg tacgctcctg cttttcgccg cttttgtcct tccactgctc gctggtggcg
     5761 atattgagat tggtgacggc gtcgccgccg gtggtgtacc gcgtctcggg gtcgcgtccg
     5821 aggttgccga gcaggatcac cttgttgacg gaagccatgt cgcgttcctt tgcctgttcc
     5881 gattgatcgc gccgcccggc gcccccattt gaggtcattg gcccggaaat gtccgaatgc
     5941 ggcgcattgt gcacgcgttc gacgcgcgcg atcgggctcg cgaaacgcaa tatactagca
     6001 ggttgccctt cttcgctttc ggtcgtgtgg cctagtcctg acgttcgatt gcgcacttgt
     6061 acggaagtcc atggaccaca tccgcattcg cggcgcgcgc acgcacaatc tcaagaatct
     6121 cagcctcgac ctgccgcgcc accggctgat cgtggtcacc gggctgtcgg ggtcgggcaa
     6181 gagctcgctc gcgttcgaca cgctctacgc cgaggggcag cgccgctacg tcgagtcgct
     6241 gtcggcgtac gcgcgccagt tcctgcagct gatggagaag cccgacgtcg acctgatcga
     6301 agggctgtcg ccggcgatct cgatcgagca gaaggcgacg tcgcacaatc cgcgctcgac
     6361 ggtcggcacc gtcaccgaga tccacgacta cctgcggctc ctgttcgcgc gcgtcggcga
     6421 tccgtattgc atcaaccatc cggagcagaa gctttcggca atgaccattt cgcaaatggt
     6481 cgacgcgacg ctcgcgatgc ccgaagacac gcggctcatg atcctcgcgc cggtcgtcgt
     6541 caaccgcaag ggcgagcagg tcgatctgtt tgccgagctc cgcgcgaagg gcttcgtgcg
     6601 cgtgcgcgtc gacggcaccg tgtacgagat cgacgcggtg ccgaagctcg cgaagaacac
     6661 gaagcacacg gtcgacgtcg tcgtcgaccg gctcaaggtg cgtccggaat tcaagcagcg
     6721 gctcgccgaa tcgtacgaga cggcgctgcg ccatggcgac agccgcgccg tcgccgtcga
     6781 gatggacggc ggccgcgagc atctgtactc ggcgaagttc gcgtgcccga tctgcaacta
     6841 ttcgctgtcg gagctcgagc cgcggctct
//
LOCUS       DASSVX010000343         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_715157, whole genome shotgun sequence.
ACCESSION   DASSVX010000343 DASSVX010000000
VERSION     DASSVX010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715157"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VFO53_14720"
     CDS             complement(<1..1250)
                     /locus_tag="VFO53_14720"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET8877579.1"
                     /translation="MAEIRNYTMNFGPQHPAAHGVLRLVLELDGEVIERADPHIGLLH
                     RATEKLAETRTWIQSLPYMDRLDYVSMMSNEHAYCLAIERLLNIDVPIRALYIRTMFD
                     EITRILNHLMWLGSHALDIGAMTPFLYAFREREDLLDMYEAVSGARMHAAYYRPGGVY
                     RDLPDRMPQYQPSKMRNARELAERNENRQGSLLDFIEDFTRRFPGNVDDYETLLTDNR
                     IWKQRTVGIGVVTAERAVALGFTGAMLRGSGVPWDLRRKQPYAVYDRMAFEIPVGVNG
                     DCYDRYLVRIEEMRQSNRIVRQCIAWLRDNPGPVIVDNYKVAPPPREGMKENMEELIH
                     HFKLFSEGMHVPAGEVYAAIEHPKGEFGVYAISDGANKPYRIKLRPPDYVHLSALDEM
                     SRGHMIADVVAIIGTQDIVFGSIDR"
     gene            complement(1252..1920)
                     /locus_tag="VFO53_14725"
     CDS             complement(1252..1920)
                     /locus_tag="VFO53_14725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET8877580.1"
                     /translation="MTAERPPQGAKSDPAAAQPQRSPPAATDALVAALAASFDEGTLR
                     STGVDRGEVTVVVDVARYLDVMRTLRDRPQLRFDMLIDLCGVDYSSFGGGAYEGRRFA
                     AVSHLLSLPHNWRLRVRTFAAEDDFPVVPSLVDVWPAVNWFEREAFDLYGIMFPGHPD
                     LRRILTDYGFVGHPFRKDFPISGYVEMRYDPEQGRVIYQPVTIEPREVTPRVIREENY
                     AENE"
     gene            complement(1917..2396)
                     /locus_tag="VFO53_14730"
     CDS             complement(1917..2396)
                     /locus_tag="VFO53_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HET8877581.1"
                     /translation="MAIEGVLDKGFVTTTLDSVINWARNGSMWPMTFGLACCAVEMMH
                     AGAARYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILQLQNKIWRTNTIARP"
     gene            complement(2415..>2596)
                     /gene="ndhC"
                     /locus_tag="VFO53_14735"
     CDS             complement(2415..>2596)
                     /gene="ndhC"
                     /locus_tag="VFO53_14735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET8877582.1"
                     /translation="YYLVAILFILFDLEIAFLFPWAVVLRDIGYFGFVAMLVFLAILV
                     VGFIYEWKKGALEWE"
BASE COUNT          449 a          838 c          868 g          441 t
ORIGIN      
        1 cggtcgatgg atccgaacac gatgtcctgc gtgccgatga tcgcgacgac gtcggcgatc
       61 atgtggccgc gcgacatctc gtcgagcgcg gacaagtgca cgtagtcggg cggccgcagc
      121 ttgatccggt acggcttgtt ggcgccgtcc gagatcgcgt agacgccgaa ctcgcccttc
      181 ggatgctcga tggccgcata cacttcgccg gccgggacat gcatgccttc ggagaagagc
      241 ttgaagtggt ggatcagctc ttccatgttc tccttcatgc cctcgcgcgg cggcggcgcg
      301 accttgtagt tgtcgacgat cacgggtccc ggattgtcgc gcagccacgc gatgcactgg
      361 cgtacgatgc gattcgactg tcgcatctcc tcgatgcgca cgaggtagcg gtcgtagcaa
      421 tcgccgttga cgccgaccgg aatttcgaac gccatccggt cgtacaccgc gtacggctgc
      481 ttgcgccgaa ggtcccacgg cacgcccgag ccgcgcagca tcgcgccggt gaagccgagc
      541 gcaacggcgc gctctgcagt cacgacgccg atgcccaccg tgcgctgctt ccagatgcgg
      601 ttgtcggtga gcagcgtctc gtaatcgtcg acgttgccag gaaagcgccg ggtgaagtcc
      661 tcgatgaaat cgagcagcga cccctgccgg ttttcgttgc gctcggcaag ctcgcgggcg
      721 ttgcgcatct tcgacggctg gtactgcggc atgcgatcgg gcaggtcgcg gtagacgccg
      781 cccggccggt agtacgcggc gtgcattcgc gcgccggaca ccgcctcgta catgtcgagc
      841 agatcctcgc gctcgcgaaa cgcgtacagg aacggcgtca tcgcgccgat gtcgagtgca
      901 tgcgagccga gccacatcag gtggttcagg atgcgcgtga tctcgtcgaa catcgtgcgg
      961 atgtagagcg cgcggatcgg cacgtcgatg ttcagcagcc gctcgatcgc gaggcagtac
     1021 gcgtgctcgt tggacatcat cgacacgtag tcgagccggt ccatgtacgg cagcgactgg
     1081 atccacgtcc gggtttcggc gagcttttcg gtcgcccggt gcaacagccc gatgtgcgga
     1141 tcggcgcgct cgatcacctc gccgtcgagc tcgagcacga ggcgcagcac gccgtgcgcc
     1201 gccggatgct gcggcccgaa gttcatggtg tagttgcgga tctcggccac gttattcgtt
     1261 ttccgcgtaa ttctcttcgc ggatcacgcg cggcgtcact tcgcgcggct cgatcgtcac
     1321 cggctggtag atgacgcgcc cctgctcggg gtcgtagcgc atctcgacat aaccggaaat
     1381 cgggaagtcc ttgcggaacg gatggccgac gaagccgtag tcggtgagga tgcggcgcag
     1441 gtcggggtga ccggggaaca tgatgccgta caggtcgaac gcctcgcgct cgaaccagtt
     1501 cacggcgggc cagacgtcga cgaggctcgg taccaccggg aaatcgtctt cggcggcgaa
     1561 cgtgcgcacg cgcaggcgcc agttgtgcgg aagcgagagc agatgcgaga ccgccgcgaa
     1621 gcgccggccc tcgtacgcgc cgccgccgaa gctcgagtag tcgacgccac agaggtcgat
     1681 cagcatgtcg aagcgcagct gcgggcgatc gcgaagcgtg cgcatcacat cgaggtaacg
     1741 cgcgacgtcg acgacgaccg tgacctcgcc gcggtcgacg ccggtgctgc gcagcgtgcc
     1801 ttcgtcgaac gacgcggcca gcgcggcgac gagcgcgtcg gttgccgcgg gtggactccg
     1861 ctgcggctgc gccgctgccg ggtccgattt cgcgccttgt ggcggccttt ctgcagtcat
     1921 ggacgcgcga tcgtgttggt gcgccagatc ttgttctgca gctgcaggat gccgtagagc
     1981 agcgcctcgg cggtgggcgg gcagcccggc acgtagatgt cgaccgggac gatgcgatcg
     2041 cagccgcgca cgaccgagta cgagtagtgg tagtagccgc cgccgttggc gcacgagccc
     2101 atcgagatga cccagcgcgg ctcggccatc tggtcgtaga ccttgcgcag cgccggagcc
     2161 atcttgttgc atagcgtccc ggccacgatc atcacatcgg actgccgcgg gctcggccgg
     2221 aacaccacgc cgaaccggtc gaggtcgtac cgcgcggcac ccgcatgcat catctccacc
     2281 gcgcagcagg cgaggccgaa cgtcatcggc cacatcgagc cgttgcgcgc ccagttgatg
     2341 acgctgtcga gcgtcgtcgt cacgaagccc ttgtccagca ccccctcaat cgccatggcc
     2401 gaaccttcct ctgcctattc ccattcgagc gcacccttct tccactcgta gatgaagccg
     2461 acgacgagga tcgcaaggaa cacgagcatc gccacgaacc cgaagtagcc gatgtcgcgc
     2521 agcacgacgg cccagggaaa gaggaacgcg atctcgaggt cgaacaggat gaagaggatc
     2581 gcgacgaggt agtagc
//
LOCUS       DASSVX010000344         9485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_48464, whole genome shotgun sequence.
ACCESSION   DASSVX010000344 DASSVX010000000
VERSION     DASSVX010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48464"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /gene="aceE"
                     /locus_tag="VFO53_14740"
     CDS             complement(<1..1590)
                     /gene="aceE"
                     /locus_tag="VFO53_14740"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HET8877583.1"
                     /translation="MGVMDDHDPVETQEWIDSLRAVIAYAGTDRARFLLGNLRDEARR
                     SGAQLPFIATTPYVNTISPEREAKSPGDRALEHRIRAAIRWNAVAMILRANKESSELG
                     GHIASFQSSALLYDIGFNHFWHAPGEAHGGDLVYIQGHSSPGIYARAFLEGRISEAQL
                     VNYRQETGGQGLASYPHPWLMPDFWQFPTVSMGLGPLMAIYQARFLRYLEGRGLANTR
                     ERKVWTFVGDGECDEPETLGAISLAGREKLDNLIFVINCNLQRLDGPVRGNGKIIQEL
                     EAVFRGAGWNVIKAIWGTGWDRLLAQDTSGRLVQRMEECVDGEYQTFKSRNGAYVREH
                     FFGKYPETRELVADMSDAEIWALRRGGHDPAKVYAAYAAAVAHREQPTVILAKTVKGY
                     GMGESGEGQMIAHQAKKMTQDALRQFRDRFDIPVSDAALPDIPFVTFAKGTPERDYLD
                     ARRGALGGFLPQRRRKSASLAAPPLPVFERLLNATGEREISTTMAFVQMLGMLIRDKT
                     LGRHVVPIVPDESRTFGMEGMF"
     gene            complement(1637..3064)
                     /gene="aspA"
                     /locus_tag="VFO53_14745"
     CDS             complement(1637..3064)
                     /gene="aspA"
                     /locus_tag="VFO53_14745"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HET8877584.1"
                     /translation="MTAPPTRVEHDLLGERAVPADAYYGIHTLRAVENFPITGTPISI
                     YADLLRALACIKEAAAMTNHELGLLDEVRKDAIVKACEEIRGGKLHEQFVVDVIQGGA
                     GTSTNMNANEVIANRALELMGHQRGEYQYLHANEHVNMSQSTNDVYPTALKVASYFGI
                     FRLVDAMAYLRHAFEGKALEFRDVLKMGRTQLQDAVPMTLGQEFSTYAVMLGEDEERL
                     KEASTLIREINLGATAIGTGINAPPDYAELVCRRLSALTGIPLITSPNLIEATQDCGA
                     FAQLSGVLKRVAIKLSKTCNDLRLLSSGPRAGLNEINLPPMQAGSSIMPGKVNPVIPE
                     VVNQIAFEVLGNDITVSFAAEAGQLQLNAFEPVIAHSLFKSITHLRNGCLTLADRCVQ
                     GITANRERLRSIVENSIGVVTALNPYIGYANATQIAQEALASGRSVYDLVLERNLLSK
                     ARLDAILQPDVLTRPRPMLEPERGE"
     gene            3240..3812
                     /locus_tag="VFO53_14750"
     CDS             3240..3812
                     /locus_tag="VFO53_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET8877585.1"
                     /translation="MIADTLIRAASLLTTIGLAASASGEHFRLTSADFTPGATLNDEQ
                     AFDGMGCTGRNVSPALRWSGAPAGTKSFALLLHDPDAPTGGAGWWHWIAYDIPAQTTE
                     LRKGAGKPDGSAMPAGAASVANDYGTPGYGGPCPPVGDKPHRYVFTLYALKVDKLELP
                     RGAHAPVVGFVVNANALGKASITGMYGRAK"
     gene            complement(3820..4182)
                     /locus_tag="VFO53_14755"
     CDS             complement(3820..4182)
                     /locus_tag="VFO53_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8877586.1"
                     /translation="MANPFVHVELHTKDLPKARQFYEKLFGWDLKDMPMPGGGSYTLI
                     GVGEGVGGGMMADKDVPPHWLAYVGVDDVRAKTAQAKSLGADVLQDVMEVGEYGTMSV
                     IRDPSGAVIALWQGKGGA"
     gene            complement(4451..5584)
                     /locus_tag="VFO53_14760"
     CDS             complement(4451..5584)
                     /locus_tag="VFO53_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8877587.1"
                     /translation="MSRMMLFVWLLHFYVGARILPGLPFGAAGFAAGIVVLVASASLI
                     PYGFRRRRSVAAGPPKALMWAGLVALGALSTLVVLTALRDAALLAALGVDALWPGAIA
                     IDTYVHWSAVAVPAVTALLTLIGYVNARRVASVRNVDIPVASLHPSLHGFTIAQITDV
                     HIGPTISRDYVEAVVDVVNGLGADLVAITGDLVDGSVPELAHQTAPLARLTARHGSYF
                     VTGNHEYYSGAHAWIGELRRLGITVLMNEHVVLDHDGADMAVAGVTDVSAHHFDASHR
                     SDPARAIAGAPDACVKLLLAHQPRSAQAASNAGFDLQLSGHTHGGQFLPWNFFVRFQQ
                     PFTAGLARLGGLWIYTSRGTGYWGPPKRLGAPSEITRLRLVPA"
     gene            5682..6401
                     /locus_tag="VFO53_14765"
     CDS             5682..6401
                     /locus_tag="VFO53_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HET8877588.1"
                     /translation="MTTVSMKPSRGRRAAMRALAGAAMLVAGRSLAQRDDVATRLPAC
                     VLTPQQTEGPYFVDERLERADIRSDPSSGTVRAGVPLALSMRISTLTGSRCEPLRGAI
                     VDVWHCDAAGIYSDVDDASLRTKGSAFLRGYQVTDARGQVRFTTIYPGAYRGRAVHIH
                     LKVRTNGGGRAAEFTSQLYFDDGLTDRVHERPPYGGAGLRRTRNVDDGLFRAGGRALI
                     LDARQDGEGYAGAYDVGLRGG"
     gene            complement(6412..7449)
                     /locus_tag="VFO53_14770"
     CDS             complement(6412..7449)
                     /locus_tag="VFO53_14770"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET8877589.1"
                     /translation="MMTVRQLFGIDVPIIQAPMAGVQASALAVAVSNAGGLGSLPCAM
                     LPADGLAQELAAIVAQTSRPFNVNFFCHTPPPVDADREAAWRAALSPYYREFGIDPAA
                     IKPGPGRAPFNADAADVLERVRPAVVSFHFGLPSDDLLARVRAAGARIVASATTVDEA
                     RWLDARGVDAIIAQGLEAGGHRGNFLSDDLTAQVGTLALVPQVVRAVRVPVIAAGGIA
                     DARGVQAMLAMGAAAVQVGTAFMCCPEATTSRIHRDALASDAAHHTAITNVFTGRPAR
                     GIVNRIMRELGPLCARAPAFPLAPAALAPLRAEAERRGSGDFSPLWSGQNASGCRTVA
                     AATLLRDLAGA"
     gene            7482..8015
                     /locus_tag="VFO53_14775"
     CDS             7482..8015
                     /locus_tag="VFO53_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET8877590.1"
                     /translation="MPRDVVYTIGHSTRSAEAFVGLLEAHGVTCLADVRTVPRSRHNP
                     QFNGDALAATLAPHAIGYERMQGLGGFRKTTPDSPNAGWRNLSFRGYADYMQTPAFAE
                     HLDALIALARDSTVAIMCAEAVPWRCHRSLIADALTVRGIRAEEIVSETKTSRHELTP
                     FARVDGTAIRYPALAND"
     gene            8137..9282
                     /locus_tag="VFO53_14780"
     CDS             8137..9282
                     /locus_tag="VFO53_14780"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HET8877591.1"
                     /translation="MPPLVIRGRALLPIVQGGMGVGVSAHRLAGTVARHGGVGTISSV
                     DLRRHHPDLMRATLHCRDRATIDRANLVALDREIIAARAIADGRGMLAVNVMRAVSEY
                     GSYVRQACESGADAIVVGAGLPLDLPELTSAHPNVAVIPILSDARGVQLVLRKWERRK
                     RLPDAIVIEHPRYAGGHLGAASIEDLADPRFDFERVIPAVLEMFRAMGLANAAIPLIP
                     AGGINSPARVRELFALGASGVQLGTAFAVTEEGDAAPEFKAVLANASAADLVEFVSVA
                     GLPARAVRTPWLDRYLRHLPAMQAVARRKPRCTLHFDCLTRCGLRDGIAQIGQFCIDT
                     QLAAALRGDVAKGLFFRGAAALPFGREIRPVRDLIEHLLPGLGATPA"
BASE COUNT         1476 a         3255 c         3249 g         1505 t
ORIGIN      
        1 gaacatccct tccatgccga acgtgcgcga ctcgtcgggc acgatcggca cgacgtgcct
       61 gccgagcgtc ttgtcgcgga tcagcatgcc gagcatctgc acgaacgcca tcgtcgtcga
      121 gatttcgcgc tcgccggtcg cgttgagcag ccgctcgaac accggcagcg gcggcgctgc
      181 gagcgacgcc gacttgcgcc gccgctgcgg caggaatccg ccgagtgcgc cgcggcgcgc
      241 gtcgaggtag tcgcgctcgg gcgtgccctt cgcgaacgtg acgaacggaa tgtcgggcaa
      301 cgccgcatcg gacaccggga tatcgaagcg atcgcgaaac tgccgcagtg cgtcctgcgt
      361 catcttcttc gcctggtgcg cgatcatctg gccctcgccc gattcgccca tgccgtaacc
      421 cttgacggtc ttggcgagga tgaccgtcgg ctgctcgcga tgcgcgacgg cggcggcgta
      481 ggcggcatac accttcgccg ggtcgtggcc gccgcggcgc agcgcccaga tctcggcatc
      541 gctcatgtcg gcgacgagct cgcgtgtctc gggatacttg ccgaagaaat gctcgcgcac
      601 gtacgcgccg ttgcggctct tgaacgtctg gtactcgccg tcgacgcatt cctccatccg
      661 ctgcacgagg cggccactcg tgtcctgcgc aagcagccgg tcccagcccg tgccccagat
      721 cgccttgatg acgttccagc ccgcgccgcg gaacacggcc tcgagctcct ggatgatctt
      781 gccgttgccg cgcacgggac cgtcgagccg ctgcaggttg cagttgatga cgaagatcag
      841 gttgtcgagc ttctcgcggc cggcgagcga gatcgcgccg agcgtctcgg gctcgtcgca
      901 ttcgccgtcg ccgacgaacg tccatacctt gcgctcgcgg gtgttcgcaa gcccgcggcc
      961 ctcgagatag cgcaggaacc gcgcctggta gatcgccatc agcggcccga gccccatcga
     1021 caccgtcggg aactgccaga agtcgggcat cagccacgga tgcggatacg acgcgagccc
     1081 ctgcccgccc gtttcctggc ggtagttcac gagctgcgct tcgctgatgc ggccttcgag
     1141 aaaggcgcgc gcgtagatgc cgggcgagct gtggccctgg atgtacacga ggtcgccgcc
     1201 gtgcgcttcg cccggtgcgt gccagaaatg gttgaagccg atatcgtaga ggagcgccga
     1261 cgactggaag ctcgcgatgt gtccgccgag ctccgacgat tccttgttgg cacgcaggat
     1321 catcgccacg gcgttccagc ggatggcggc gcggatgcgg tgctcgaggg cgcggtcgcc
     1381 gggcgacttc gcttcgcgtt ccggcgaaat cgtgttgacg tacggtgtgg tcgcgatgaa
     1441 cggcagctgg gcgccgctgc gtcgtgcctc gtcgcgcagg ttaccgagca ggaagcgcgc
     1501 tcgatcggtg ccggcgtagg cgatgaccgc gcgcagcgaa tcgatccatt cctgcgtttc
     1561 tacgggatcg tgatcgtcca tgacgcccat cgtgctcctc cggcgcaacg gaaccgggtg
     1621 tgtcccggac gctgcgctac tctccgcgct cgggctcgag catcggccgc ggccgcgtca
     1681 gcacatccgg ctgcaggatc gcatcgaggc gcgccttcga cagcaggttc cgctcgagca
     1741 cgaggtcgta gacactgcgg ccgctggcaa gcgcttcctg tgcaatctgc gtcgcgttcg
     1801 cgtagccgat gtacggattc agcgcggtca ccacgccgat cgagttctcc acgatgctgc
     1861 gcagccgctc gcgattggcc gtaatgccct gcacgcagcg gtcggcgagc gtgaggcaac
     1921 cgttgcgcag atgggtgatg ctcttgaaca ggctgtgcgc gatgaccggc tcgaacgcgt
     1981 tcaactgaag ctgcccggcc tcggcggcga agctcaccgt gatgtcgttg ccgaggacct
     2041 cgaacgcaat ctggttgacc acttccggaa tgaccgggtt caccttgccg ggcatgatgc
     2101 tcgagcccgc ctgcatcggc ggcaggttga tctcgttcag ccccgcgcgc gggccgctcg
     2161 acagcaggcg caggtcattg cacgtcttgg agagcttgat cgcaacgcgc ttcagcacgc
     2221 ccgacaattg cgcgaacgcg ccgcagtcct gcgtcgcctc gatcagattc ggcgacgtga
     2281 tcagcggaat gccggtcagc gccgacagac ggcggcatac gagctccgca tagtcgggcg
     2341 gcgcgttgat gccggtgccg atcgccgtcg cgccgagatt gatctcgcgg atcagcgtcg
     2401 acgcctcctt caggcgctcc tcgtcctcgc ccagcatgac cgcgtaggtc gagaattcct
     2461 ggccgagcgt catcggcacc gcatcctgca actgcgtgcg gcccatcttc agcacgtcgc
     2521 ggaattccag ggccttgccc tcgaaggcat ggcgcagata cgccatcgca tcgacgagac
     2581 ggaagattcc gaagtacgac gccaccttga gcgccgtcgg gtacacgtcg ttcgtgctct
     2641 ggctcatgtt gacgtgctcg ttggcgtgca ggtactggta ctcgccgcgc tggtggccca
     2701 tcagctcgag tgcgcgattg gcgatcacct cgttcgcgtt catgttggtc gacgtgccgg
     2761 cgccgccctg gatcacgtcg acgacgaact gctcgtgcag ctttccgccg cggatctcct
     2821 cgcaggcctt gacgatcgcg tccttgcgta cctcgtcgag caggccgagc tcgtggttgg
     2881 tcatcgccgc cgcttccttg atgcaggcga gcgcgcggag gagatcggcg tagatcgaaa
     2941 tcggcgtgcc ggtgatcggg aaattctcga ctgcgcgcag cgtatggatg ccgtagtacg
     3001 cgtccgcggg aacggcgcgc tcgccgagaa gatcgtgctc gacgcgagtc ggaggtgcgg
     3061 tcatgggagg ggacccggcg gcaccgggct tcgaggcggt ggcccgccgg aatggcaaat
     3121 gtacgcgatt cggggccgac gggcgacgtg gctgcgccgt tgcaattctt gcaacatggc
     3181 gcaaaagtgc acgatgcgcg caaaatgttc ggcgtcaccg tcacgactgg agcaacttca
     3241 tgatcgccga cacgcttatc cgcgccgctt cgctgctgac cacgatcggg ctcgcagcct
     3301 ccgcttccgg tgaacacttc aggttgacga gcgccgactt cacgccgggc gcaacgctga
     3361 acgacgaaca ggcgttcgac ggcatgggct gcaccggacg aaacgtatcg ccggcgttgc
     3421 gctggtcggg cgcgccggcg ggcacgaaga gcttcgcgct gctgctgcac gatcccgatg
     3481 caccgaccgg tggcgccggg tggtggcact ggatcgcgta cgacatccct gcgcagacga
     3541 ccgagttgcg caagggcgcc ggcaagcccg acgggtcggc gatgcccgcg ggggctgcga
     3601 gcgtcgccaa cgattacggc acgccgggct atggcgggcc ctgtccgccc gtgggcgaca
     3661 agccgcaccg ctacgtgttc acgctctacg cgctgaaggt ggacaagctc gagctgccgc
     3721 ggggcgcgca tgcgccggtg gtcggcttcg tcgtgaatgc gaatgcgctc ggcaaggcgt
     3781 ccatcaccgg catgtacggg cgcgcgaagt agcccacgct caggcgccgc ccttgccctg
     3841 ccacagcgcg atgaccgcgc cggacgggtc gcggatcacg ctcatcgtgc cgtattcgcc
     3901 gacctccatc acgtcctgca gcacgtcggc cccgagggat ttcgcctgcg cggtcttcgc
     3961 gcgcacgtcg tcgacgccga cgtaggcgag ccagtgcggc ggcacgtcct tgtcggccat
     4021 catgccgccg cccacacctt cgccgacgcc gatcagcgtg tagctgccac cgccgggcat
     4081 cggcatgtcc ttcaggtccc agccgaagag tttctcgtag aactggcggg ccttcggcag
     4141 atccttcgta tgcagttcga cgtgcacgaa cgggttggcc atcgcgatct ccttcgcttc
     4201 aggggaatga acactttgcc gtgcggcacg cgacgcttgc gtgcgtgcga caccgcacgc
     4261 cggctcgagg cgacgccggc gccggtctga cgcgacggcg cgacgacagc cgctggcact
     4321 tccgggcgca tgtgcccgga gtcgtactgt acggcgaaac ggccggttgc gaaggattcg
     4381 acgtgaaaac ggaagtggcg ctcgccatgt tcgcggcgtt gccgggcggc cgcgcgagcg
     4441 gcgaacgatg tcaggcgggt acgagccgca gccgcgtgat ctccgacggc gcgccgagac
     4501 gcttcggcgg cccccagtag ccggtgccgc ggctcgtgta gatccacagg ccaccgagtc
     4561 gcgcgagccc cgcggtgaac ggctgctgga agcggacgaa aaaattccat ggcaggaact
     4621 gtccgccatg cgtgtggccg gaaagctgaa gatcgaatcc cgcgttcgag gcggcctgcg
     4681 ccgaacgtgg ctggtgcgct agcagcagct tgacgcacgc gtccggcgcg ccggcgatgg
     4741 cccgcgcggg gtcgctgcga tgcgacgcat cgaaatgatg cgccgacacg tcggtcacgc
     4801 ccgcaacggc catgtcggca ccgtcgtgat cgagcaccac gtgctcgttc atcaggacag
     4861 tgatgccgag gcggcgcaat tcaccgatcc acgcatgcgc cccggagtaa tactcgtgat
     4921 tgccggtgac gaagtagctt ccatggcgcg ccgtcaggcg cgcgagaggc gccgtctggt
     4981 gcgcaagctc tggaacgctg ccgtcgacga ggtcgccggt gatcgcgacg agatcggcgc
     5041 caagcccgtt cacgacgtcg acgaccgcct cgacgtagtc gcgcgaaatc gtcggcccga
     5101 tgtgcacgtc ggtgatctgc gcaatcgtga aaccgtgcag cgacgggtgc agcgacgcga
     5161 cgggaatatc gacgttgcga acgctcgcca cgcgtcgcgc gttgacgtag ccgatcaacg
     5221 tgagcaatgc ggtgaccgcg ggaaccgcca ccgcgctcca gtgcacgtaa gtgtcgatcg
     5281 cgatcgcgcc gggccacaac gcgtcgacgc caagggctgc gagcaatgcg gcgtcgcgca
     5341 gcgccgtgag cacgaccagc gtcgacagcg cgccaagggc gacgagtccc gcccacatga
     5401 gcgctttcgg ggggccggcg gcgaccgagc ggcggcggcg aaagccatag ggaatcagcg
     5461 atgccgatgc gacgagcacg acgatgcccg ccgcaaaacc cgcggcgccg aatggcaaac
     5521 cgggcagtat gcgggccccg acatagaagt gcagcagcca gacgaaaagc atcatgcgcg
     5581 acattggcgc taaactcgat cgaggcgcgt ggatcgcgca ggcacttccg acaggtgggg
     5641 cttgtcgaac agtcgcacaa gcccgaccga cgaggaaccc gatgacgacc gtttccatga
     5701 agccgtcgcg cggccgccgc gcggcgatgc gtgcgctcgc cggcgctgcg atgctggtcg
     5761 ccggccggtc gctggcgcaa cgcgatgatg tcgcgacgcg cctgcctgcg tgcgtgctga
     5821 cgccgcaaca aaccgagggg ccctacttcg tcgacgagcg gctcgagcgc gccgacatcc
     5881 gcagcgaccc gtccagcgga accgtccgtg ccggcgtgcc gctcgcgttg tcgatgcgca
     5941 tctcgacgct gaccggaagc cgctgcgagc cgctgcgcgg cgcaatcgtc gatgtctggc
     6001 attgcgacgc cgcgggaatc tattccgacg tcgacgacgc gagcctgcgc acgaagggca
     6061 gcgcgttcct tcgcggctac caggtgaccg atgcgcgcgg gcaggtgcgc ttcacgacga
     6121 tctacccggg cgcctatcgc gggcgcgcgg tgcacattca cctcaaggtg cgcacgaacg
     6181 gcggcggtcg cgctgccgag ttcacgtcgc agctctattt cgacgatgga ctcaccgatc
     6241 gcgtccatga aaggccacct tatggcggcg ccggcttgcg caggacgcgc aacgtcgacg
     6301 acggtctgtt tcgcgccggc ggtcgcgcgc tgatcctcga cgcccggcag gatggcgaag
     6361 gctacgccgg cgcgtacgac gtgggtctgc gcggcggttg agcgccgcgc gtcaggcgcc
     6421 ggcgaggtcg cgcagcagcg ttgccgccgc gacggtgcgg cacccgctcg cgttctgtcc
     6481 cgaccacagc ggcgagaagt cgcccgaccc gcgccgctcg gcctcggcgc gaagcggtgc
     6541 gagcgcggcc ggtgcgagcg gaaacgctgg tgccctggcg caaagcggac cgagctcgcg
     6601 catgatgcga ttcacgatgc cgcgcgccgg ccgccccgtg aatacgttcg tgatcgcggt
     6661 gtgatgcgcc gcatcgctcg cgagcgcatc ccggtgaatg cggctcgtcg tcgcttcggg
     6721 acagcacatg aacgccgttc cgacctgaac ggcagcggcg cccatcgcga gcattgcctg
     6781 cacgcccctg gcgtcggcga tgccgccggc ggcgatgacc ggcacccgca ccgcgcgcac
     6841 gacctgcggc accagcgcca gcgtgccgac ctgcgccgtc aggtcgtcgg aaagaaagtt
     6901 gccgcgatgg ccgcccgctt cgaggccctg cgcaatgatt gcgtcgacgc cgcgggcgtc
     6961 gagccagcgt gcctcgtcca ccgtggtggc cgacgcgacg attcgcgcac cggcggcgcg
     7021 tacgcgggcg agaaggtcgt cggacggcag gccgaaatgg aagctcacca ccgcgggccg
     7081 cacccgctcg agaacgtccg cggcgtcggc gttgaacggt gcgcgtccgg gacccggctt
     7141 gatcgcggcc ggatcgatgc cgaattcgcg gtaatagggc gacagcgccg cccgccatgc
     7201 cgcctcgcga tcggcatcga ccggcggcgg cgtgtgacag aagaaattga cgttgaacgg
     7261 ccgcgatgtc tgcgccacga tcgccgccag ttcctgcgcg aggccgtcgg ccggcagcat
     7321 cgcgcacggc agcgagccga gcccgccggc gttcgacacc gcgaccgcaa gcgcactcgc
     7381 ctgcacgccg gccatcggcg cctggatgat cggcacgtcg atgccgaaga gctgtcgcac
     7441 tgtcatcatc gctcctttcc cctgggcgaa gggtgggttc catgccgcga gacgtcgtct
     7501 acacgatcgg tcattcgacg cgcagcgccg aggcgttcgt cgggctgctc gaagcccacg
     7561 gcgtcacgtg cctcgccgac gtgcggacgg tgccgcgctc gcgccataac ccgcagttta
     7621 acggcgacgc gctcgctgcg acgctcgcgc cgcacgcgat cgggtacgaa cggatgcagg
     7681 ggctgggcgg ctttcgcaag accacacccg actcgccgaa tgccggatgg cgcaacctgt
     7741 cgtttcgcgg ttatgccgac tacatgcaaa cgccggcctt tgccgagcat ctcgatgcgc
     7801 tgatcgcact cgcccgcgat tcgaccgtcg ccatcatgtg cgcggaagcg gtcccgtggc
     7861 gctgccaccg ctcgcttatc gcggatgcgc tcaccgtgcg tggcattcgc gccgaggaaa
     7921 tcgtgagcga aacgaagacg tcgcggcacg agctgacgcc gttcgcacgc gtcgacggga
     7981 cggcgattcg ctatcccgcg ctcgccaacg attgatttcg atcaaaggcg cccgggggag
     8041 cgccgcgtat gctcgccaag ctcccctttc gccggcctcg ccgtcgagcg gcgcgccttc
     8101 gcctgatcga tgccgaccga accctccgca tccaccatgc cacccctcgt gattcggggg
     8161 cgcgcactgc tgccgatcgt gcagggcggc atgggagtcg gcgtctcggc gcatcggctc
     8221 gcgggcaccg tcgcgcggca cggcggcgtc ggcacgatct cgtcggtcga cctgcgccgt
     8281 caccatcctg acctgatgcg cgcgacgctg cactgccgcg accgcgcgac gatcgaccgc
     8341 gcgaacctcg tcgcgctcga ccgcgaaatc atcgccgctc gcgcgatcgc ggacgggcga
     8401 ggcatgctcg ccgtcaacgt gatgcgcgcg gtgtccgaat acggaagcta cgtgcggcag
     8461 gcctgcgaaa gcggcgccga tgcgatcgtc gtcggcgcgg gcttgccgct cgacctgccc
     8521 gagctcacgt cggcgcatcc gaacgtggcg gtgatcccga tcctctcgga tgcgcgcggc
     8581 gtgcagctcg tgctgcgcaa gtgggagcgc aggaagcggt tgcccgacgc aatcgtcatc
     8641 gagcatccgc gctacgccgg cggccatctc ggcgccgcca gcatcgagga ccttgccgat
     8701 ccgcgtttcg acttcgagcg cgtcattccg gcggtgctcg agatgttccg ggcgatgggc
     8761 ctcgccaacg ctgcgatacc gctcattccg gccggcggca tcaactcgcc cgcgcgggta
     8821 cgcgagctct tcgcgctcgg cgcgagcggc gtgcagctcg gcaccgcgtt cgcggtcacc
     8881 gaggagggcg atgcggcgcc cgaattcaag gcagtgctgg cgaatgcgag cgccgccgac
     8941 ctggtcgaat tcgtgagcgt cgccggcctc ccggcgcgcg ccgtgcgcac gccgtggctc
     9001 gaccgctacc tccggcacct cccggcaatg caggcagttg cgcgtcgcaa gccgcgctgc
     9061 acgctccact tcgattgcct cacgcgctgc ggcctgcgcg acggcatcgc gcagattggc
     9121 cagttctgca tcgacacgca actcgccgcc gcgctgcgcg gcgacgtcgc gaaggggctg
     9181 ttcttccgcg gtgcggccgc gctgccgttc ggccgcgaaa tccggccggt gcgcgatttg
     9241 atcgagcatc tgctgcccgg gttgggcgca acgccggcgt agagccgcag tcaggcggca
     9301 cctaccgcat cggccgctcg tgcgtgtcgc gagcggcacc taccgcattg gccgctcgtg
     9361 cccgtcgcgg tacttttcgg cgcaccggat gacgccgatg ttcgtgacga actcgagaca
     9421 ggcccgcgcg tcggcgtcgg catgcaaccc ggcgtggtcg ggcgcgggcc gccccggggg
     9481 ccggg
//
LOCUS       DASSVX010000345        14846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_165363, whole genome shotgun sequence.
ACCESSION   DASSVX010000345 DASSVX010000000
VERSION     DASSVX010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 14846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14846
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165363"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1935)
                     /gene="glgX"
                     /locus_tag="VFO53_14785"
     CDS             complement(<1..1935)
                     /gene="glgX"
                     /locus_tag="VFO53_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET8877592.1"
                     /translation="MKPFTAVWPGRSLPRGAHWDGEGVNFAVFSERAEAVELCVFDRT
                     GRRELQRIPLVERTDEVFHCYLPEARPGLLYGYRMHGPYRPEQGLRFNAHKLLLDPYA
                     RSIAGTLRWSDALFGYNVGHKRGDLSFDRRDSASAMPKSKVVEPAFTWGNDQPPNIAW
                     HDMVIYELHVRGFTMQHPDVPPALRGTYAGLATAPVIDYLKRLGVTTIELMPVHYFVD
                     DRRLVQQDLRNYWGYNTIGYFAPESRYSASGKLNEFKTMVKTLHSAGFEVILDVVYNH
                     TAEGNELGPTLCFRGMDNAAYYRLQADPRYYMDYTGCGNTLNMQHPRVLQLIMDSLRY
                     WVTDMHVDGFRFDLAPALARELHEVDRLGAFFDILRQDPVLDRVKLIAEPWDLGEGGY
                     QVGNFPPGWAEWNDKYRDTMRSYWRGDGGVIGEFARRLTGSSDLYGRSGRAPHASINF
                     ITAHDGFTLRDLVSYNAKHNEANLEDNRDGSDNNISWNCGVEGATADEGVLALRARQS
                     RNLLATLLLSQGIPMLTAGDEIARSQGGNNNAYCQDNPVSWVDWTLDDARSNLLAFVR
                     RVVELRRQHPAFRRRHFFEGRSLTGKDDKDIAWLKPDGSEMTEQEWNNDFARCLGVFL
                     AGDRLGETDARGRAVSDDDYL"
     gene            complement(1988..2695)
                     /locus_tag="VFO53_14790"
     CDS             complement(1988..2695)
                     /locus_tag="VFO53_14790"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877593.1"
                     /translation="MDNVNMLVEPIRESLHQIGVFLPRLLLAIVVLIIGWLVAKLVRF
                     AIVKALHAINFNVVTEKAGIDRFLSQGGADIDTIRVLGGLFYWLVILAALMVAFNSLD
                     LAYVTDLIGRIVLYVPRVMVAVVILVFGAYFARFIATALATYLRNIGAREAGLMGRFA
                     LYAIMAFVIMIALDQIGLGDIIRETFLVIVAAVALAFALAFGLGGTKRAGELIERWSR
                     QDADDRRDARPPTKSVL"
     gene            complement(2761..6087)
                     /gene="treS"
                     /locus_tag="VFO53_14795"
     CDS             complement(2761..6087)
                     /gene="treS"
                     /locus_tag="VFO53_14795"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HET8877594.1"
                     /translation="MADPATAALAAPADAPPDAARWYKDAIIYQLHIKAFYDSNADGV
                     GDFRGLTEKLDYIRDLGVNTIWLLPFYPSPLRDDGYDVADYRNVNPLYGTRADFQHFV
                     REAHKHELRVITELIVNHTSDQHPWFQAARRAPKGSNKRDYYVWSDDPTKYSGTRIIF
                     TDTEKSNWAWDEVANAWYWHRFFSHQPDLNFDNPQVLKAIFRVMRFWLDMGVDGFRLD
                     AIPYLCEREGTNNENLPETHAVLKRLRALIDAQYPNVLLLAEANQWPEDVREYFGDGD
                     ECHMAYHFPLMPRMYMAIAQEERHPITEIMAQTPDIPASCQWAIFLRNHDELTLEMVT
                     SRERDYMYNMYAADKRARINLGIRRRLAPLLENDMGRIKLMNSLLLSMPGSPTLYYGD
                     EIGMGDNIYLGDRDAVRTPMQWAPERNAGFSRADPQRLYLQPIMDPVYGFEAVNVEAQ
                     WRAPSSLLHWTRRMLEVRKGFAAFGRGTLTFLRPDNRKILAYLRECGDETILCVANLA
                     RTAQPVELDLANYKGRVPIELLGRTAFPPIGDVPYLMTLAGHAFLWFRLASGQQVSRR
                     DEQVPYQELPVLVLFDGWASLFRDRVVPWRIGMSEKVRNQLESDALPAFVASRRWYAA
                     KSAPVRRVEIADHVEWKPGARSWLVTIARVDSGGEAQAYFLPLALAWEDFDESLVRAL
                     SPLAIAKVRQQAQVGVLADAFGDESFVRALVAAVGAAAVQKSARGTLHFRPTTAFAAL
                     AGTGYGALPVTTPGATSSNTIAALADRLFVKGYRRLQVGVNPELEIGRYLTEVVRFRH
                     SVPVAGSVDYVADDGRTATLALVQGYVQNQGDGWDYTHNYLERFFDDTSREAPAAKGA
                     DVHGAYTTLARTLGMRTADLHLAFAQSSGDPAFDPEPMTSTDVAEWAERVRQEAMRGL
                     ERLERRIGTLDPALRSAAERLLAQREPLLARIARHASDRSDGPKTRVHGDYHLGQVLL
                     VQNDFVITDFEGEPARPLAERARKQSPLKDVAGMLRSFDYALHATLFEFRTERADVRE
                     RVDEAGRQWRAQATRAFLDGYDEIAKAAGLASARGEATGLLELFVLEKALYELGYEAD
                     NRPEWLGIPIHGLLGILDGT"
     gene            complement(6103..8127)
                     /locus_tag="VFO53_14800"
     CDS             complement(6103..8127)
                     /locus_tag="VFO53_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HET8877595.1"
                     /translation="MAARKGNARKTDAFPAEGRARAVIEGIAPQVDGGRFAVKRIVGD
                     RVVVEADCFADGHDVLACRLRFRREDEAAWSESPMDALGNDRWRGEFIVADVGRYSYT
                     VSAWVDAFLSWRHDFARRVDADDMRVAAQMGADIIAAAALRARGDERKRLTAWARELR
                     DTSDVDALRDLALDDVRAALAQRHPDRRFETAYPVEFALVVDRLRAGFSSWYELFPRS
                     TAQREQVHGTFADCEARLPYVAQMGFDVLYLPPIHPIGRERRKGRNNALVAGPDDVGS
                     PWAIGSVEGGHKALNPQLGTMDDFRRLAASARSLGLEIALDIAFQCAPDHPYVEAHPE
                     WFRHRPDGSVQYAENPPKKYQDIYPFNFEPDEWRALWAELYDVMAFWIAEGVRIFRVD
                     NPHTKPFAFWEWAIARIKREHPDVILLSEAFTRPKVMHRLAKLGFTQSYTYFTWRNNK
                     HELTEYFTELGSAPGRDYFRPNCWPNTPDILAESLQIGGRPAFMTRLVLAATLCANYG
                     IYGPAFELLEHVPREPGSEEYLHSEKYELRHWDLERADTLAPFIARVNSARRDNRALQ
                     HGADLVFFPTDNDEILCCGRIDADRSNAMIVVVNLDPHHVQSGWVTLDLGALGIEPEQ
                     PFEVHDLLTDARYAWHGSRNFVLLDPARVPAHLFRVRRRVRNEHDFVYFD"
     gene            complement(8129..10045)
                     /gene="glgB"
                     /locus_tag="VFO53_14805"
     CDS             complement(8129..10045)
                     /gene="glgB"
                     /locus_tag="VFO53_14805"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET8877596.1"
                     /translation="MIEATAGPTFGVDNKTGLGEQDVYLFREGTHASLHALLGCALSD
                     GSARFRVWAPNAARVSVIGDWNAWNGEANRLVVRGDETGIWEGTVRGVTRGAAYKYRI
                     VTRDGAILEKADPFALYAEVPPATASRAWSLDYAWGDGTWMASRRSRNALDAPMSIYE
                     LHFGSWRRDDPGQLPTYRGMAEPLADYLCAQGFTHVEFLPLMEHPFYGSWGYQTTGYF
                     APTARYGTPQDLMYLIDVLHRRGIGVILDWVPSHFPDDPHGLAWFDGTHLYEHADPRK
                     GFHPEWKSLIFNYDRHEVRAFLASSALFWLDKYHVDGLRVDAVASMLYLDYARNAGEW
                     VPNVHGGKENLGAIAFLRQLNEAVYRQHPDVQTFAEESTSWPMVSRPTFAGGLGFGMK
                     WNMGWMHDTLAYMHNEPIHRKHHHDRLTFSIWYAFSENFVLPLSHDEVVYGKGSLIGK
                     MPGDPWQRFANLRALYGYMWAHPGKKLLFMGSEFAQWREWSHEAALDWPLLAHAEHAG
                     VQRFVSDVNRVYRATPALHEIDFEMPGFEWIVGDDREASVVAFLRRARGGAPVLVACN
                     FTPVPRLNYAVGVAEAGFWREMLNSDATAYGGSGMGNLGGVHSVPVGAHGRFHSLTLT
                     LPPLSTVFLEFGGA"
     gene            complement(10235..12796)
                     /gene="glgP"
                     /locus_tag="VFO53_14810"
     CDS             complement(10235..12796)
                     /gene="glgP"
                     /locus_tag="VFO53_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET8877597.1"
                     /translation="MDTRGTKYGIEVNPVVPKPLARLTDLAGDLWYSWDRPTRELFAR
                     LHPGLWNAAGHSPKAFLKRVDQDRLDAAAKDPVFLYKFHRILSTHDTYEGIATPATDG
                     VALGAGDHVAYFCAEFGFHESLPIYSGGLGILAADHCKAASDANLPLTGVGLLYRQGY
                     FSQTIDAEGNQHANYADSSFADLPVELVRGEDGREMRVAVSLPGRDVSVRLWRARAGH
                     VMLVLLDTDVAENGENDRLITYRLYGGDRTTRIEQEIVLGIGGVRALALLGVTPTVWH
                     MNEGHAAFMVLERIRMLVQAGTPFDTALEAVAASNVFTTHTPVAAGHDQFASDVIEQY
                     FASYCSDVGVAAATLLELGRSPDSDDFNMTALAVRGSRLHNGVSRIHGEVTSRTLQGL
                     WPDVPPAENPVGYVTNGVHVRTFLALEWFDLFDRYLGPEWVHRFGSSAEWDDIDRIPD
                     HLFWSVHQHLKSSMLEVVGERVRAQHLRNQGSEAHLDRILRHARPDDPTVLTVGFARR
                     FATYKRATLLFNDLDWLRAITSDAKRPVVFIFAGRAHPADVPGQDLIREIARVAKLPE
                     FEGTILLVEGYDLRLARRLVAGVDVWLNNPIYPLEASGTSGMKAGMNGIINLSVQDGW
                     WGEGYQPGNGWTIKPATGVSDAARRDSDEARSLYEILQNHALPLYYDRVPAGYSPAWL
                     RMAKRSMATLLPRFNSRRMLNEYVENLYSHAATQGRRYMADERAVAGAVAAFKRRVRD
                     AWPRVALRVVDSGAQRIAFGEAFAIEVAVQLAGLDPSDVVVELLLERLDERTGRGERR
                     QYPLNAVDGVNEAGEHRYRIELAPELCGHLSYRLRAYPCHRELTHPHETGLMRWA"
     gene            13052..>14846
                     /locus_tag="VFO53_14815"
     CDS             13052..>14846
                     /locus_tag="VFO53_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET8877598.1"
                     /translation="MHAYWCAANYLSAGQIYLRDNPLLRRPLQIGDIKRRLLGHWGTT
                     PGLNFVYVHLNRIIREHALDMMFVTGPGHGAPGVLANTYLEGTYSELYPDVSRDERGL
                     ARFFTQFSFPGGIPSHTAPETPGSINEGGELGYSLAHAFGAAFDNPQLLVACVVGDGE
                     AETGALATSWHSNKFLDPVRDGAVLPVLHLNGYKIAGPTVLARIPHDELEALLRGYGY
                     RPYFVEGDDPLPMHVRMASTLDKIVGEIRRIQHDARERGFRQRPQWPMIVLRSPKGWT
                     GPKVVDGKQIEGTFRAHQVPLADFDAHPGHLELLERWLRSYEPERLFDAQGRLVTALA
                     ELAPQGERRMSANPHANGGVLLRDLELPDLRNYAVAVPGPGHVDAEATRVQGRLIRDV
                     LRANADARNFRVFSPDETASNRWDAVFEATDRCSTAQIEPGDDHVAPDGRVMEMLSEH
                     QCEGWLEGYLLTGRHGMFNCYEAFVHIVDSMFNQHAKWLESSRKIAWRRPIASLNYLL
                     TSHVWRQDHNGFSHQDPGFIDYVVNKKAEVIRVYLPPDANTLLSVTDHCLRSRNYVNV
                     IVAGKQPEQQWLTIEQAAAHCAEGLGIWEWAS"
BASE COUNT         2471 a         4965 c         4965 g         2445 t
ORIGIN      
        1 gaggtaatcg tcgtcggaaa cggcgcgccc gcgcgcgtcg gtttcgccga gccggtcgcc
       61 ggccaggaac acgccgaggc agcgcgcgaa gtcgttgttc cattcctgct cggtcatctc
      121 gctgccgtcg ggcttcaacc acgcgatgtc cttgtcgtcc ttcccggtga gcgagcggcc
      181 ctcgaagaaa tgccgccgcc ggaacgccgg gtgctgccgg cgcagctcga cgacgcggcg
      241 cacgaacgcg agcaggttgc ttcgcgcgtc gtccagcgtc cagtcgaccc acgagaccgg
      301 gttgtcctgg caatacgcgt tgttgttgcc gccttgcgag cgcgcgatct cgtcgccggc
      361 cgtcagcatc ggaatgccct gcgacagcag cagcgtcgcg agcaggttgc gcgactggcg
      421 cgcgcgcagc gccaggacgc cttcgtcggc ggtggcgcct tcgacgccgc agttccacga
      481 gatgttgttg tcgctgccgt cgcggttgtc ctcgagattg gcctcgttgt gctttgcgtt
      541 gtaggaaacg aggtcgcgca gcgtgaaccc gtcgtgcgcc gtgatgaagt tgatgctcgc
      601 gtgcggcgcg cggccgctgc gtccatagag gtcggacgag ccggtgagcc gccgcgcgaa
      661 ctcgccgatg acgccgccgt cgccgcgcca atacgagcgc atcgtgtcgc ggtacttgtc
      721 gttccattcg gcccatcccg gcgggaagtt gccgacctgg tagccgccct cgccgagatc
      781 ccacggctcg gcgatcagct tgacgcgatc gagcaccggg tcctggcgca ggatgtcgaa
      841 gaacgcgccc agccggtcga cttcatgcag ctcgcgcgcg agtgccgggg caaggtcgaa
      901 gcggaagccg tcgacgtgca tgtcggtcac ccagtagcgc agcgaatcca tgatgagctg
      961 cagcacgcgc ggatgctgca tgttgagcgt gttgccgcag cccgtgtagt ccatgtagta
     1021 gcgcggatcg gcctgcagac ggtagtaggc ggcgttgtcc atgccgcgga agcaaagcgt
     1081 cggaccgagc tcgttgcctt ccgccgtgtg gttgtagacg acgtcgagga tcacctcgaa
     1141 tccggccgaa tgcagcgtct tcaccatcgt cttgaactcg ttcagcttgc ccgacgccga
     1201 atagcgcgat tcgggcgcga aatagccgat cgtgttgtag ccccagtagt tgcgcaggtc
     1261 ctgctgcacg agccgccggt cgtcgacgaa gtagtgcacg ggcatcagct cgatcgtcgt
     1321 gaccccgagc cgcttcagat aatcgatgac cggcgcggtg gcgagaccgg cgtaggtgcc
     1381 gcgcagcgcc ggcggcacgt cgggatgctg catcgtgaac ccgcgcacgt gcaactcgta
     1441 gatcaccatg tcgtgccacg cgatgttcgg cggctggtcg ttgccccacg tgaacgccgg
     1501 ctcgacgacc ttgctcttcg gcatggcgct cgcgctgtcc cggcggtcga acgacaggtc
     1561 gccgcgcttg tggccgacgt tgtagccgaa cagtgcgtcg ctccagcgca acgtgccggc
     1621 gatgcttcgc gcataagggt cgagcagcag cttgtgcgcg ttgaagcgca gtccctgctc
     1681 cggccgatac gggccgtgca tacgataacc gtacagcagg cccggacgcg cctccggcag
     1741 gtagcagtgg aagacctcgt cggtgcgctc gacgagcggg atgcgctgca attcgcggcg
     1801 cccggtgcga tcgaacacgc agagctcgac cgcttccgcg cgctccgaaa acacggcgaa
     1861 attgacaccc tcgccgtccc agtgggcgcc gcgcggcagc gagcgtcccg gccatacggc
     1921 ggtgaacggc ttcatcgggc cgacgtggtg cggccggggt gccggatgga aacgcgcggc
     1981 acggcgatca cagcacggac ttcgtcggcg gccgcgcgtc gcgcctgtcg tcggcgtcct
     2041 gccgcgacca gcgctcgatg agctcgcctg cgcgcttcgt gccgccgagc ccgaacgcca
     2101 gcgcgaacgc cagcgcgacg gcggcgacga tgacgaggaa ggtctcgcgg atgatgtcgc
     2161 cgaggccgat ctggtcgagc gcgatcatga tgacgaacgc catgatcgcg tagagcgcga
     2221 agcggcccat gagccccgcc tcgcgcgcgc cgatgttgcg cagatacgtc gcgagcgccg
     2281 tcgcgatgaa gcgtgcgaaa tacgcgccga acacgaggat cacgaccgcc accatcactc
     2341 tcggcacgta cagcacgatg cgcccgatca ggtcggtcac gtacgcgagg tcgaggctgt
     2401 tgaacgcgac catcagtgcc gccaggatca cgagccagta gaagagcccg ccgagcacgc
     2461 ggatggtgtc gatgtcggcg cctccctgcg acaggaagcg gtcgatgccc gccttctcgg
     2521 tgaccacgtt gaagttgatc gcgtgcagcg ccttcacgat ggcgaagcgc acgagctttg
     2581 cgacgagcca gccgatgatg agcacgacga tggcgagcag aaggcgcggc aggaagacgc
     2641 cgatctgatg cagcgattcg cggatcggct cgaccagcat gttgacgtta tccatgacgt
     2701 tctcccatgt gcgaccgcgc gaaccggcgg agcgtgctca ccctcgatgc gggagcgcga
     2761 tcacgtcccg tcgaggatcc cgagcaggcc gtgtatcgga atgcccagcc actcgggccg
     2821 attgtcggct tcgtacccga gctcatacag ggctttttcg agcacgaaca gctcgagcag
     2881 ccccgtcgct tcgccgcgtg ccgaagcaag tccggccgcc tttgcgatct cgtcgtagcc
     2941 gtcgagaaag gcgcgcgttg cctgcgcgcg ccactgccgc ccggcctcgt cgacacgctc
     3001 gcggacgtcg gcgcgctcgg tcctgaattc gaagagcgtc gcatgcagcg cgtaatcgaa
     3061 cgagcgcagc attcccgcaa cgtccttcag cggcgactgc ttgcgcgcgc gctcggcgag
     3121 cggccgcgca ggctcgccct cgaagtcggt gatcacgaag tcgttctgca cgagcagcac
     3181 ctggcccaga tgatagtcgc cgtgcacgcg cgtcttcggc ccgtcgctgc gatcggacgc
     3241 gtggcgcgcg atccgcgcga gcagcggctc gcgctgcgcg agcagccgct cggccgcgct
     3301 gcgcagcgcc gggtccagcg tgccgatccg ccgttcgagc cgctcgaggc cgcgcattgc
     3361 ctcctggcgc acacgctcgg cccattcggc gacgtcggtg gaggtcatcg gctcgggatc
     3421 gaacgccgga tcgccactgc tttgcgcgaa ggcgagatgc aggtccgcgg tgcgcatgcc
     3481 gagcgtgcgc gcgagcgtcg tgtaggcgcc gtgcacgtcg gcgcccttcg ccgccggcgc
     3541 ctcgcgcgag gtgtcgtcga agaaccgctc gaggtagttg tgcgtgtaat cccagccgtc
     3601 accctggttc tgcacgtagc cctggaccag cgcaagcgtc gccgtgcgcc cgtcgtcggc
     3661 cacgtagtcg acgctgcccg cgaccgggac gctgtgccgg aagcgcacga cttcggtgag
     3721 gtagcggccg atctcgagtt cgggattcac gccgacctga aggcgccggt accccttgac
     3781 gaacaggcgg tcggcgagtg ctgcgatcgt gttgctgctc gtcgcccccg gcgtcgtgac
     3841 gggaagcgcg ccgtaaccgg ttccggcgag cgctgcgaac gccgtcgtcg ggcggaaatg
     3901 aagcgtgccg cgcgccgact tctgcaccgc ggccgcgccg accgcagcca cgagcgcacg
     3961 cacgaacgat tcgtcgccga acgcatcggc aagcacgccg acctgcgcct gctggcgcac
     4021 cttcgcgatc gccagcggcg acagcgcgcg cacgagcgac tcgtcgaaat cttcccatgc
     4081 gagcgcaagc ggcaggaaat acgcctgcgc ttcgccgccc gaatcgacac gggcgatcgt
     4141 cacgagccac gaccgcgcgc ccggcttcca ttcgacatgg tcggcgatct cgacccggcg
     4201 cacgggcgcg ctcttcgccg cataccagcg ccggctcgcg acgaacgccg gcagcgcatc
     4261 ggactcgagc tggttgcgca ccttttccga catgccgatc cgccacggca cgacacgatc
     4321 gcgaaagagg cttgcccagc cgtcgaacag cacgagcacc ggcagctcct ggtagggcac
     4381 ctgctcgtcg cgtcgggaca cctgctggcc ggacgcgagg cgaaaccata ggaacgcgtg
     4441 gccggcgagc gtcatcaggt agggcacgtc gccgatcggc ggaaaggccg tgcgcccgag
     4501 aagctcgatg ggcacgcgtc ccttgtagtt ggcgagatcg agctcgaccg gctgcgccgt
     4561 gcgtgcgaga ttggccacgc acaggatcgt ctcgtcgccg cactcgcgca ggtacgcgag
     4621 gatcttccgg ttatccgggc gcaggaacgt gagcgtgccg cggccgaatg cggcaaagcc
     4681 cttgcgcacc tccagcatcc ggcgcgtcca gtgcagcagc gacgacggcg cacgccactg
     4741 cgcctcgacg ttcaccgctt caaatccgta aaccggatcc atgatcggct gcaggtacag
     4801 ccgctgcgga tcggcgcgcg aaaagcccgc gttgcgctcg ggcgcccatt gcatcggcgt
     4861 gcgcacggcg tcgcgatcgc cgaggtagat gttgtcgccc atcccgatct cgtcgccgta
     4921 atagagcgtc ggcgacccgg gcatcgacag gagcaggctg ttcatcagct tgatgcgccc
     4981 catgtcgttc tcgagcagcg gggcgagcct tcgccggata cccagattga tgcgcgcgcg
     5041 cttgtcggcc gcgtacatgt tgtacatgta gtcgcgctcg cggctcgtca ccatctcgag
     5101 cgtcagctcg tcgtggttgc gcaggaagat cgcccattgg cagcttgccg gaatgtcggg
     5161 cgtctgcgcc atgatctcgg tgatcggatg ccgctcttcc tgcgcgatcg ccatgtacat
     5221 gcgcggcatc agcgggaagt gataggccat gtggcactcg tcgccgtcgc cgaagtactc
     5281 gcgcacgtcc tccggccact ggttcgcctc ggcaagcagc agcacgttcg gatactgcgc
     5341 gtcgatcagc gctcgcagcc gcttcagcac cgcatgcgtc tcgggcaggt tctcgttgtt
     5401 cgtgccctcg cgctcgcaca ggtacgggat cgcatcgagg cggaatccgt ccacccccat
     5461 gtcgagccag aagcgcatga cgcggaagat cgccttcagc acctgcgggt tgtcgaagtt
     5521 gagatcgggc tggtggctga agaagcggtg ccaataccac gcgttcgcaa cctcgtccca
     5581 ggcccagttc gacttttcgg tgtcggtgaa gatgatccgc gtgccgctgt acttcgtggg
     5641 gtcgtcgctc cacacgtagt agtcgcgctt gttcgacccc tttggcgcgc ggcgcgccgc
     5701 ctggaaccaa ggatgctggt cggaggtgtg attgacgatc agctcggtga tgacacgaag
     5761 ctcgtgcttg tgcgcctcgc gcacgaaatg ctggaagtcg gcgcgcgtgc cgtacagcgg
     5821 attgacgttg cggtaatcgg ccacgtcgta gccgtcgtcg cgcagcggtg acggatagaa
     5881 cggaagcagc cagatcgtgt tgacgccgag atcgcggatg taatcgagct tttcggtcag
     5941 gccgcggaag tcgcccaccc catcggcgtt ggaatcgtag aacgccttga tgtggagctg
     6001 gtagatgatg gcgtccttgt accagcgcgc cgcatcgggc ggcgcgtcgg ccggtgcggc
     6061 aagtgctgcg gtggcagggt cggccaatca gatcctcagg gcctagtcga agtagacgaa
     6121 gtcgtgctcg ttgcgcacgc ggcggcgcac gcggaacagg tgtgccggca cgcgcgccgg
     6181 atcgagcagc acgaagttgc gcgacccgtg ccatgcgtag cgcgcgtcgg tgagaaggtc
     6241 gtgcacctcg aacggctgct cgggctcgat acccagtgcc ccgaggtcga gcgtcaccca
     6301 gcccgattgc acatgatgcg ggtcgaggtt gacgacgacg atcatggcgt tcgatcggtc
     6361 ggcgtcgatg cgcccgcagc agaggatctc gtcgttgtcg gtcgggaaga acacgagatc
     6421 ggcgccatgc tgcagcgcgc gattgtcgcg ccgcgccgag ttgacgcgcg cgatgaacgg
     6481 cgcgagcgtg tccgcgcgct cgaggtccca gtggcgcagc tcgtactttt cggagtgcag
     6541 gtattcctcg gagcccggct cgcgcggcac gtgttcgagc agctcgaacg cggggccgta
     6601 gatgccgtaa ttggcgcaga gcgtggcggc gaggacgagc cgcgtcatga acgcgggacg
     6661 cccgccgatc tgcaggcttt ccgccaggat gtcgggtgtg ttcggccagc agttgggacg
     6721 gaagtagtcg cggccgggtg cactgccgag ctcggtgaag tactcggtga gctcgtgctt
     6781 gttgttgcgc caggtgaagt acgtgtacga ctgcgtgaag ccgagcttag cgagccggtg
     6841 catcaccttc ggccgcgtga acgcctccga cagcaggatg acgtcggggt gctcgcgctt
     6901 gatccgcgcg atcgcccatt cccagaacgc gaacggcttc gtgtgcggat tgtcgacgcg
     6961 gaagatgcgc acgccttccg cgatccagaa cgccatcacg tcgtagagct cggcccagag
     7021 cgcgcgccac tcgtcgggtt cgaagttgaa cggatagatg tcctggtatt tcttcggtgg
     7081 attctcggcg tactgcacgc tcccgtccgg gcgatgccgg aaccactcgg gatgcgcttc
     7141 gacgtacggg tgatcgggcg cgcactggaa cgcgatgtcg agcgcgatct cgaggccgag
     7201 cgatcgcgcg ctcgccgcga ggcggcgaaa atcgtccatc gtgccgagct gcggattcag
     7261 cgccttgtgg ccgccctcca cggaaccgat cgcccatggg ctgccgacgt cgtcggggcc
     7321 ggcgacgagc gcgttgttgc ggcccttgcg ccgctcacgg ccgatcgggt gaatcggcgg
     7381 caggtacagc acgtcgaagc ccatctgggc aacgtagggc aggcgcgcct cgcaatcggc
     7441 gaacgtgcca tgcacctgct cgcgttgcgc cgtcgagcgc gggaagagct cgtaccagct
     7501 cgagaacccc gcgcggagcc gatcgacgac cagcgcaaat tcgaccggat aagccgtctc
     7561 gaagcgccgg tcgggatggc gctgtgcaag cgccgcgcgc acgtcgtcga gtgccaagtc
     7621 gcgcaaggca tcgacatccg acgtgtcgcg caactcgcgc gcccacgcag tcaaccgctt
     7681 gcgctcgtcg ccgcgcgcgc gcaatgcggc cgccgcgatg atgtcggcgc ccatctgtgc
     7741 cgccacgcgc atgtcgtcgg catcgacccg gcgcgcgaag tcgtgccgcc acgagagaaa
     7801 cgcatcgacc catgcgctga ccgtatagct gtagcggccg acgtcggcca cgatgaattc
     7861 gccgcgccag cgatcgttgc ccagcgcatc catcggcgac tcgctccacg cggcctcgtc
     7921 ctcgcggcga aagcgcaacc ggcaggcgag cacatcgtgt ccgtcggcga agcagtcggc
     7981 ctcgacgacg acccggtcgc cgacgatgcg cttgacggcg aagcgtccac cgtcgacctg
     8041 cggcgcgatg ccctcgatga cggcgcgcgc gcggccctcg gcggggaacg cgtcggtctt
     8101 gcgcgcattt cccttgcgtg ccgccacgtt acgcccctcc aaactcgagg aacacggtcg
     8161 aaagcggcgg cagcgtcagc gtcagcgagt ggaagcggcc gtgcgctccc acgggtaccg
     8221 agtgcacgcc gcccaggttg cccatgccgc tgccgccgta ggcggtcgcg tcgctgttca
     8281 gcatctcgcg ccagaatccg gcttccgcga cgccgactgc gtaattgagg cgcggcaccg
     8341 gcgtgaaatt gcaggccacc agcaccggtg ccccgcctcg cgcgcggcga agaaacgcga
     8401 cgacgcttgc ctcgcggtca tcgccgacga tccactcgaa gcccggcatc tcgaagtcga
     8461 tctcgtgcag cgccggtgtc gcacgataga cgcggttcac gtcggacacg aagcgctgca
     8521 cgccggcgtg ctcggcgtgc gcgagcagcg gccagtcgag cgcagcctcg tgcgaccact
     8581 cgcgccactg cgcgaactcg ctgcccatga acagcaattt cttgccggga tgcgcccaca
     8641 tgtaaccgta cagcgcgcgc aggttcgcga accgctgcca ggggtcgccg ggcatcttgc
     8701 caatcagcga tcccttgccg tacacgactt cgtcgtgtga cagcggcagc acgaagtttt
     8761 ccgagaacgc gtaccagatc gagaacgtga gccggtcgtg atgatgcttg cgatggatcg
     8821 gctcgttgtg catgtacgca agcgtgtcgt gcatccatcc catgttccat ttcatcccga
     8881 agccgaggcc cccggcgaat gtcggccgcg aaaccatcgg ccacgacgtc gattcctcgg
     8941 cgaacgtctg cacgtcgggg tgctggcggt agaccgcttc gttgagctgg cgcaggaacg
     9001 cgatggcgcc gaggttctcc ttgccgccgt gaacgttcgg cacccactcg ccggcgttgc
     9061 gcgcatagtc gaggtacagc atcgatgcga ccgcgtcgac gcgcaggccg tcgacgtggt
     9121 acttgtccag ccagaagagc gcactcgacg ccaggaacgc ccgcacctcg tgccgatcgt
     9181 aattgaagat caggctcttc cattcggggt ggaagccctt gcgcggatcg gcatgctcgt
     9241 agaggtgcgt gccgtcgaac caggccagcc catgcggatc gtcgggaaag tgcgacggca
     9301 cccagtcgag aatgacgccg atcccgcgtc gatgcagcac gtcgatcagg tacatcaggt
     9361 cctgcggcgt tccgtagcgg gccgtcggcg cgaaatagcc ggtggtctgg tagccccacg
     9421 agccatagaa cgggtgctcc atcagcggca ggaattcgac gtgcgtgaag ccctgcgcgc
     9481 acaggtaatc ggcgagcggc tcggccatcc cgcgatacgt cggcaattgt ccgggatcgt
     9541 cgcggcgcca tgacccgaag tgcaactcgt agatcgacat cggcgcgtcg agcgcattgc
     9601 gcgaccggcg cgatgccatc cacgtgccat cgccccacgc atagtcgaga ctccaggcgc
     9661 gcgaagcggt tgccggagga acctctgcat acaatgcgaa cgggtcggcc ttttccagga
     9721 tggcgccgtc gcgcgtgacg atccggtact tgtaggcagc cccgcgcgtc acgccgcgca
     9781 cggtgccttc ccagataccg gtttcgtcgc cgcgcacgac caggcgattc gcctcgccgt
     9841 tccacgcgtt ccagtcgccg atcaccgaaa cgcgcgcggc gttcggcgcc cagacgcgga
     9901 agcgtgcaga accgtcggac agcgcgcagc ccagcagcgc atgcagcgac gcgtgcgtgc
     9961 cttcgcgaaa caggtacacg tcctgttcgc cgaggccggt cttgttgtcc acgccgaacg
    10021 tcggtcccgc cgttgcttcg atcatgccat ccgtcccttc cggccatggc gtcggtcgac
    10081 gcgagcttct cagagaagcg gacccatcgc gcgccgattg gttcaggtgc ggctgccgct
    10141 tcctcgcgca tcgacgttgg ggtcagagac gactttccgg acggttgtca ccacaccgaa
    10201 cgatggcgcc gggaaagtcg tctctgaccc cccttcatgc ccagcgcatc agtccggtct
    10261 cgtgcggatg cgtcaactcg cgatggcacg gataggcgcg caagcgatag ctcaaatgtc
    10321 cgcaaagctc gggggcaagc tcgatccggt agcgatgctc gccggcctcg ttcacgccat
    10381 ccacggcatt cagcgggtat tgccgccgct cgccgcgacc cgttcgctcg tccagccgct
    10441 cgagcagcag ctcgacgacc acgtcggacg gatcgaggcc ggccagttgc acggccactt
    10501 cgatcgcgaa tgcctccccg aaggcgatcc gctgcgcgcc gctgtcgacc acccgcaatg
    10561 cgacacgcgg ccacgcgtcg cgcacgcgcc gcttgaatgc cgcaacggcg cccgccaccg
    10621 cacgctcgtc ggccatgtac cggcgtccct gcgtcgccgc gtggctgtag agattctcga
    10681 cgtattcgtt cagcatccgc cgcgaattga agcgcggcag cagcgtcgcc atcgatcgct
    10741 tggccatgcg aagccatgcc ggcgagtaac ccgcgggcac acggtcgtag tacagcggca
    10801 gcgcatggtt ttgcaggatc tcgtagagcg agcgcgcctc gtcgctgtcg cgccgggcag
    10861 cgtcgctcac gccggttgcc ggcttgatcg tccagccgtt gccgggctgg tagccttcgc
    10921 cccaccatcc gtcctgcacc gacaggttga tgatgccgtt catgccggcc ttcatgcccg
    10981 acgtgcccga cgcttcgagc gggtagatcg gattgttgag ccatacgtcg acgccggcga
    11041 cgagacggcg cgcaagacgc aagtcgtagc cttcgacgag caggatggtt ccttcgaact
    11101 cgggcagctt cgccacccgc gcgatttccc tgatcaggtc ctgcccgggc acgtccgcgg
    11161 ggtgcgcgcg tccggcgaag atgaacacga ccggccgctt cgcatcgctc gtgatcgcgc
    11221 ggagccagtc gaggtcgttg aaaagcagcg tcgcccgctt gtacgtggcg aagcggcgcg
    11281 cgaagccgac ggtgagcacc gtcggatcgt cgggccgggc atggcgcagg atgcggtcga
    11341 gatgcgcttc gctgccctgg ttgcgcaagt gctgcgcgcg cacgcgctcg cccacgacct
    11401 ccagcatgct cgacttcaga tgctggtgca ccgaccagaa caggtggtcg ggaatgcggt
    11461 cgatgtcgtc ccactcggcg ctgcttccga accggtgcac ccactcgggt ccgagatagc
    11521 ggtcgaagag gtcgaaccat tcgagcgcga gaaacgtgcg cacgtggacg ccgttcgtca
    11581 cgtagcccac cgggttctcg gcgggcggca cgtcgggcca caggccctgc agcgtgcgcg
    11641 aggtcacctc gccgtggatg cgcgacacgc cgttgtgaag gcgcgagccg cgcaccgcga
    11701 gtgccgtcat gttgaaatcg tcgctgtcgg ggctgcgtcc gagctcgagc agcgtcgccg
    11761 ccgcgacacc cacgtcgctg cagtacgaag cgaagtactg ctcgatcacg tcggacgcga
    11821 actggtcgtg gcctgccgcc accggcgtgt gcgtcgtgaa cacgttgctc gccgccaccg
    11881 cctcgagtgc cgtatcgaac ggcgtccctg cctgcacgag catgcggatg cgctcgagca
    11941 ccatgaatgc cgcgtgacct tcgttcatgt gccagacggt cggcgtcacc ccgagcaacg
    12001 cgagcgcgcg cacgccaccg atgccgagca cgatttcctg ctcgatgcgc gtcgtgcggt
    12061 cgccaccgta aagacgatag gtgatcagcc ggtcgttctc gccgttctcg gcaacgtcgg
    12121 tgtcgagcag cacgagcatc acgtggccgg cgcgcgcacg ccacaggcgc accgagacgt
    12181 cgcggcctgg gaggctcacc gcaacgcgca tctcgcggcc atcctcgccg cgcacgagct
    12241 cgaccggcag gtcggcgaag ctcgaatcgg cgtaattcgc gtgctggttg ccctcggcgt
    12301 cgatcgtctg cgagaagtag ccctgccgat aaaggaggcc gacgcccgtc agcggcagat
    12361 tggcgtcgct tgccgccttg cagtggtcgg cagcgaggat gccgaggccg cccgagtaga
    12421 tcggcaggct ttcgtgaaag ccgaattcgg cgcagaaata cgcaacatgg tcgccggcac
    12481 cgagcgcaac gccgtcggtc gcgggcgtcg cgatgccctc gtaggtgtcg tgcgtcgaca
    12541 atatccggtg aaacttgtag aggaacaccg ggtccttcgc ggcggcatcg agtcgatcct
    12601 ggtcgacgcg cttcaggaac gccttcgggc tgtggcccgc ggcgttccac aatcccgggt
    12661 gcagccgcgc gaagagctcg cgcgtcgggc gatcccagct gtaccagaga tcgcccgcaa
    12721 ggtcggtcag gcgcgcgagc ggcttgggaa cgaccggatt gacttcgatg ccgtacttcg
    12781 taccgcgggt atccatgctg atgctggtcc agatgcgaga attcgaaaga atcggggcta
    12841 acgcgcgcga tgcgcgtcgg tcattccatc gcggcaagcc gccgcgttgc cgtcgagcct
    12901 gacacgggcc gttgccggcg cggcaattct agacgagcgg cgtatcctcg cacggtaaaa
    12961 cccgacttgc gcgatgcatg cggaggcttg agtgcaaccc gaagccacga atcagcgaga
    13021 cacgacactg cccggcgagc tgctgcaccg gatgcacgcg tactggtgtg ccgcgaatta
    13081 cctttcggcc gggcagatct atctgcgcga caacccgctg ctgcgccggc cgctgcagat
    13141 cggcgacatc aagcggcgtc tgctcggcca ctgggggacg accccggggc tcaacttcgt
    13201 ctacgtgcat ctcaaccgca tcatccgcga gcacgcgctc gacatgatgt tcgtcaccgg
    13261 tcccggtcac ggtgcgcccg gcgtgttggc gaacacgtat ctcgagggaa cgtacagcga
    13321 gctctacccc gacgtgtcgc gcgacgagcg aggtcttgca aggttcttca cgcagttttc
    13381 gtttcccggc ggcatcccga gccacacggc gccggaaacc ccgggatcga tcaacgaagg
    13441 cggtgagctc gggtacagcc tcgcgcacgc attcggcgcc gcattcgaca atccgcagct
    13501 gctggtcgcg tgcgtggtgg gcgacggcga agcggagacc ggcgcgctcg ccacgagctg
    13561 gcactcgaac aagtttctcg atccggtgcg cgacggcgcg gtgcttcccg tattgcacct
    13621 gaacggctac aagatcgccg gcccgacggt gctcgcgcgc attccgcacg acgagctcga
    13681 ggcgctgctg cgcggctacg ggtaccggcc gtacttcgtc gaaggcgacg atccgctacc
    13741 gatgcacgtg cggatggcgt cgacgctcga taagatcgtc ggcgagattc gacgcatcca
    13801 gcacgacgcg cgcgagcgcg gattcaggca acgcccgcaa tggccgatga tcgtgcttcg
    13861 ctcgccgaag gggtggaccg gtccgaaggt tgtcgacggc aagcagatcg agggcacgtt
    13921 tcgcgcacat caggtgccgc tcgccgattt cgacgcgcac cccggacacc tcgaacttct
    13981 cgaacggtgg ctgcgaagct acgagcccga gcggcttttc gacgcgcagg ggcgactcgt
    14041 tacagcgctc gccgagctcg cgccgcaggg cgaacggcgg atgagtgcga acccgcacgc
    14101 gaatggcggc gtgttgctgc gcgacctcga gctgccggac ttgcgcaact atgcggtcgc
    14161 ggtgccgggc ccggggcacg tcgacgccga agcgacgcgc gtccagggca ggttgatccg
    14221 cgacgtgctg cgcgcgaacg ccgacgcgcg caacttccgc gtcttcagcc ccgacgaaac
    14281 cgcatcgaac cgctgggacg ccgtgttcga agccaccgat cgctgctcga ctgcacagat
    14341 cgagcccggc gacgatcacg tggcgccgga cgggcgcgtg atggaaatgc tgagcgagca
    14401 ccagtgcgag gggtggctcg aaggctatct gcttaccggc cgccacggca tgttcaactg
    14461 ctacgaggcg ttcgtccaca tcgtcgattc gatgttcaac cagcacgcca agtggctcga
    14521 atcgtcgcga aagatcgcgt ggcggcgtcc gatcgcctcg ctcaattacc tgctgacgtc
    14581 gcacgtgtgg cgccaggatc acaacggctt ttcgcaccag gatcccggct tcatcgacta
    14641 cgtcgtcaac aagaaggccg aggtcattcg cgtctacctg ccgcccgacg ccaacacgct
    14701 gctctcggtg accgaccact gcctgcgcag ccgcaactac gtcaacgtga tcgtcgccgg
    14761 caagcagccc gagcagcagt ggctcacgat cgagcaagcg gccgcgcatt gcgccgaagg
    14821 cctcggcatc tgggagtggg cgtcga
//
LOCUS       DASSVX010000346        11883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_415392, whole genome shotgun sequence.
ACCESSION   DASSVX010000346 DASSVX010000000
VERSION     DASSVX010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 11883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11883
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415392"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFO53_14820"
     CDS             complement(<1..628)
                     /locus_tag="VFO53_14820"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HET8877599.1"
                     /translation="MLNARSLWSLTRESAAQWSADFAPSMGAALAYYTIFSIAPLLVI
                     AIAVAGFFFGADAARGEIFGQLSGLIGERGAATIEAAVASASQTGHGTFAAIAGIVAL
                     LLGATTVFGELQSDLDRIWDTPRPEHAGIRGLLRGRLLSFGMVLGIGFLLLVSLVLSA
                     ALSAIGHFWSGALPGWEVLLQIVNFVVGFVVVSGLFAMIYKFLPRCDIG"
     gene            complement(837..2603)
                     /locus_tag="VFO53_14825"
     CDS             complement(837..2603)
                     /locus_tag="VFO53_14825"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877600.1"
                     /translation="MKKALVIAVCALALVLPAAGADMRKTLHVAFPVAESGFDPQAIS
                     DTYSDAVCLAIFDPLYRYDYFARPVVLQPNTADGLPQVSDGGRTYTIKVKRGIRFAAD
                     PAFGGKPRELTAGDYVYSIKRVFDPKLRSYWLYLFEGNLVGLDAPLERARKSGRFDYD
                     EPIDGLQAIDRYTLRIRFREPDYAFGWWLTTSSFAAVAREVVERYQDESHRVMEHPVG
                     TGAYRLESWTRGQRILLAANPDFRDERYPAPGNDAADRAIAKDLTGRKLPLAGHVDIA
                     IIEEAQPRMLSFDRGELDYVAVPATLAPTVLDADRLRPALAKRGVALHRGPEPSLSFF
                     FFNMDDPVVGGYEPAKVALRRAIAMAYDRAADIRQLANGQAVRADQPVPPGLFGHDAS
                     IGMVVPFDPQGARTLLDRFGYKDRNGDGYRETPDGKPLVIVKGSTTDADARASDELWK
                     RSMDAIGVRMTFLKNKWPELNKMTEAGHMMMWGLGWISGIPDGDPFYSYLVSRNIGTS
                     NDARLRLPAYDRLYDQAHVLPDGDERNAAFRKLNELIVGYAPWIVNQYPYRNELTQPW
                     LRGFKLNAFQRSQWAYYDVAAH"
     gene            complement(2600..3838)
                     /locus_tag="VFO53_14830"
     CDS             complement(2600..3838)
                     /locus_tag="VFO53_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET8877601.1"
                     /translation="MPSLPSRLAEARSAVRGLAGSKIRDIANAGMHDPDVLAFWFGEP
                     DQVTPAFIRDAATASLARGETFYSQNFGIPELRAAIAGYVGALHRPTTADEIAVTASG
                     MSALMLAVEALVAPGDRVVVVTPLWPNLVEIPKILSAEVVCVPLSFGASGWRLDLDRL
                     LDALTPGTRALMLNSPNNPTGWTIDRAAQQALLAHCARHGIWIVADDVYERLYYAEDF
                     ACAPSLLDIADGGMRVVSTNSFSKAWLMTGWRLGWIVGPKPLMPDLGKLIEYNTSCSP
                     VFVQRAGVAALRDGEPTVDATRERLRRARNRLVDGLRALPGVEVAPPEGAMYAFFRVE
                     GVDDSMAFCRRLVQEAKLGLAPGSAFGPEGEGFVRWCFAAADSRLDDGIARLARFLNG
                     QTRACTNRKVVSDPNSVRRR"
     gene            complement(3846..7061)
                     /locus_tag="VFO53_14835"
     CDS             complement(3846..7061)
                     /locus_tag="VFO53_14835"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET8877602.1"
                     /translation="MPDDNSTQRVTAAASPREWRSAGWRLRVARLLRGRGLVRLRSSI
                     GLRLALLALALGLPFVVYVGVNAARQASTAREFAQQRTLALARVVAARVDDVVGDNLI
                     ALALVRHGVTLDPSATSANDAFLDKIRGDLPGMVQNVGVWTLDGRNVGMLNRTSANAG
                     RSIADRDYFRRAVQTSKTAIEGPLASDDDGNPVVAFARPVIGATGAVAGVVTLAIDLK
                     QVQRHLDLGGAVPRGTVISIVNADGVVLARSVDPDRWIGMSVLAVGKARQHLASREGV
                     DETVGADNVPRIAGYTQAKRTPWHVFVGMPADAALSIARTNRFETLALGALSLLLGIG
                     FAARLGVRIARPLHRLAYDATLLARGNLAHRTSIGGDDETGVLASTLNRLAQTVEERT
                     RALQEKTGALEEKTTALERSRSELATITANVPVLIAYIDAGERFRFVNEYYRDVFGAS
                     PERVVGHRLRAVLHPTVYARLESRMRDVRAGMPQTFETTFAGDGRGPVFMVTCFPDYG
                     DGHEVRGAYVVCQDITRRKDAEEALAGRERFVRLIADGIPARITYIDTSNRLLFGNRR
                     FAEYWGREPADIVGRPLADVVSSAAYAQIKPELDRSYAGLARRFDLVVDRANGTEFYQ
                     VDHVPDIDAAGVVHGVVTISQDVTALRQAKQALAASEKRMRTVADNLPALIAYLDAGE
                     RYLFVNARSQQMFGLSPEAMLGQRAQDVLSPETYAQSKPHLERACRGERARFQRTVVR
                     NGRACHELVELIPDQEASGRVLGLYALVQDITDLRTAQAKAEESEERLRSMTDSMPSM
                     VGYIDCDRCYRFNNRYYETWLGRPLTEITGRRVADVLGPRAYENVAPNLDRAFAGERV
                     DFDVEVPGPEGSRFVRGSYIPDIDASGAVLGVYTSSTDITPLKEVERQLERLAQRDTL
                     TGLPNRHAFNDGIAAALRRSQRAATPVALLFLDVDGFKQINDTLGHAAGDEVLREFAR
                     RLSASVRATDLVARLAGDEFVIVLEGVHTREECRFVARKIIAAMRPEFDAGEAAVKVT
                     TSIGVALGQGGETTPEALLKRADSALYAAKGHGRDRYEIAI"
     gene            7185..7547
                     /locus_tag="VFO53_14840"
     CDS             7185..7547
                     /locus_tag="VFO53_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HET8877603.1"
                     /translation="MNPVSLALHPAVVCAALLSCSNLFMTFAWYGHLKLLENRAWWVA
                     ALISWGIALFEYLLQVPANRIGFTVLSLAQLKIMQEVITLTVFVPFAIFFMGATLKLD
                     YLWAGLCMMGAVYFIFRG"
     gene            7577..8953
                     /gene="mpl"
                     /locus_tag="VFO53_14845"
     CDS             7577..8953
                     /gene="mpl"
                     /locus_tag="VFO53_14845"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HET8877604.1"
                     /translation="MHLHILGICGTFMGGIAAIARRAGHTVTGCDANVYPPMSTQLAA
                     LGIALTEGFDAGQLDAVARRADVFVVGNVVSRGNPLMEAIMNAGRPYVSGPRWLFENV
                     LRGRFVLAVAGTHGKTTTSAMLAHILEAAGLTPAFLIGGVPVDFGVSARLTDSAFFVI
                     EADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLLRTIPGDG
                     RIVVNAGDNALARVIARGAWTPIERFDTAASTAAPAPDWTIADAGGVTLGGETIGVLD
                     FAQSGRHNRLNALAAIAAARHAGVPPHDALAALRSFRGVRRRMELRGHAGGVAVYDDF
                     AHHPTAIATTLDGLRARQAQGRILAVLEPRSNTMKLGTMKAALPASLAAADRVYCYGA
                     NLGWDAAAALAPLGARAEVHDDLRALVAAVARDARAGDHVLVMSNGGFGGVHEKLLDA
                     LAARADPA"
     gene            8962..9573
                     /locus_tag="VFO53_14850"
     CDS             8962..9573
                     /locus_tag="VFO53_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HET8877605.1"
                     /translation="MRPTIVYLHGFRSSPASAKAARLAEAVGRLPERDRPVLLIPQLN
                     PRPALAMAAILALTRDADPCGVTFVGSSLGGYYATYAAETLGARAVLVNPAVRPYDDL
                     VPYRGRQVNLYTGIAFDVTERHFEELRALAIPKITRPARYLLLVQSGDEVLDYRVAVA
                     FYASGWQQVTGGGDHAFSGFETHIPGILRFCGVGSAPGFVRRD"
     gene            9710..11041
                     /gene="rocD"
                     /locus_tag="VFO53_14855"
     CDS             9710..11041
                     /gene="rocD"
                     /locus_tag="VFO53_14855"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HET8877606.1"
                     /translation="MATHENNDLRASYSAASRRPPAARAGRDPASQPLSDAALDLIGR
                     EKAFGARNYDPLPVVLARGEGSWLADVDGHRYLDLMSAYSAVSFGHAHPRILAALVAQ
                     AQRLGVTSRAYYNDELPGLLERLALLTGLDRALPANGGAEAVETAIKAARKWGSKVKG
                     IAEGRVEIIGCDGNFHGRTIAAIGLSTEPQYRDGFGPFPPGLSTIPYGDADALARAIT
                     PRTAAFLVEPIQGEGGIVVPPPRYLARCLQICRDHHVLLICDEIQTGLGRTGRFLASE
                     HDGVKPDGVILGKALGGGLLPVSAFVATNDVMQVFHPGDHGSTFGGNPLASAVAMAAL
                     DVLFDEKLVERSAELGAWLLSELQDLRSHVVSDVRGRGLFVGIEVDARYTSARPVVDR
                     LLARGILSKDTHGTVVRIAPPLNIARDDLAWAIGEIHTVFRELERDLKRAA"
     gene            11128..>11883
                     /locus_tag="VFO53_14860"
     CDS             11128..>11883
                     /locus_tag="VFO53_14860"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HET8877607.1"
                     /translation="MRCLRLLAFLTGVFAACGLTTGAYAVSRICMSADSLSFGQRPVG
                     SSESATVVVSNCGDAPFAFTDVSRHTATNVAYRTQTSCSTGMTLAPGAQCAASVYFEP
                     TVPGQASGALWFHNTTSTPDQLLTFYGRGVDAQAGTASLVFSPAIANFGDAAVGRETA
                     PLVLTLQNAGTAPLVPSALVLNGRDPYDFRGETGTGASSCGIGRAIAPGAGCTLILYF
                     HPQASGARQANLVVDAPQLAALAVVTLAGDGMDA"
BASE COUNT         1807 a         4146 c         4051 g         1879 t
ORIGIN      
        1 agccaatgtc gcagcgcggc aggaacttgt agatcatcgc gaacaggccg ctgacgacga
       61 caaagcccac gacgaagttg acgatctgca gcagcacctc ccagcccggt agcgcgccgc
      121 tccagaagtg gccgatcgct gacagcgcag cggacagcac gagcgacacg agcagcagga
      181 agccgatgcc gagcaccatg ccgaacgaaa gcaggcgacc gcgcaacagg ccccggattc
      241 ccgcatgctc cggcctcggc gtgtcccaga tccggtcgag gtcggactgc agttcgccga
      301 agacggttgt cgcgccgagc agcagcgcga cgatgcccgc gattgccgcg aacgttccat
      361 gcccggtctg gctggcgctc gcgacggccg cctcgatggt ggccgcgccg cgctcgccga
      421 tcaatccgct gagctggccg aaaatctcgc ctcgggcggc atcggcgccg aaaaagaagc
      481 cggccacggc gatcgcgatc acgagcagcg gcgcgatcga gaagatcgtg tagtacgcaa
      541 gcgccgcgcc catgctcggc gcgaagtcgg cggaccactg cgcagccgac tccctcgtga
      601 gtgaccacag cgatcgggcg ttcagcatcg taggctctcc ctgacgcgat cggcgttcgg
      661 cacggccgac agacggccaa agccgcagcg gcgcgtgcgg cgagatcgca cccggccggt
      721 tcgaagcgta gcggtcgtgg caatcggcgc gcaatcggcg cgcgccgcgc acgagcgtcc
      781 ggcgtttgcc gacacccgac gcgtcgccgg tgacaaaccg cgggttgcgc tcgcgttcag
      841 tgcgcggcca cgtcgtaata cgcccactgc gaccgctgga acgcgttcag cttgaagccg
      901 cgcagccacg gctgcgtgag ctcgttgcgg tacggatact ggttgacgat ccacggcgcg
      961 tagccgacga tcagctcgtt cagcttgcgg aacgccgcat tgcgctcgtc gccgtcgggc
     1021 agcacgtgcg cctggtcgta caggcggtcg tacgcgggca ggcgcaggcg cgcgtcgttc
     1081 gacgtgccga tgttgcggct cacgaggtag ctgtagaacg ggtcgccgtc gggaatgccg
     1141 ctgatccagc ccaggcccca catcatcatg tgcccggctt cggtcatctt gttgagctcg
     1201 ggccacttgt tcttgaggaa cgtcatccgc acgccgatcg cgtccatgct gcgtttccac
     1261 agctcgtcgc tcgcgcgcgc gtcggcgtcg gtcgtcgatc ccttgacgat caccagcggc
     1321 ttcccgtcgg gcgtctcgcg atagccgtcg ccgttgcggt ccttgtagcc gaagcggtcg
     1381 agaagcgtcc gggcgccctg gggatcgaag gggacgacca tgccgatcga tgcgtcgtga
     1441 ccgaacaggc ccggcggcac cggctgatcg gcgcgcaccg cctggccgtt ggcaagctgg
     1501 cgaatgtcgg cggcgcggtc gtacgccatc gcgatggcgc gccgcagcgc gaccttcgcc
     1561 ggctcgtatc cgccaaccac cgggtcgtcc atgttgaaga agaagaacga cagcgacggt
     1621 tccggcccgc ggtgaagcgc aacgccgcgc ttggcgagtg ccgggcgcag gcgatcggcg
     1681 tcgagcaccg tgggcgcgag cgtcgcgggc acggccacgt aatcgagctc gccgcggtcg
     1741 aacgacagca tccgcggctg cgcttcctcg atgatcgcga tgtcgacgtg gcccgcgagc
     1801 ggcagcttgc gtccggtcag gtctttcgca atcgccctgt cggccgcgtc gttgcccggc
     1861 gccggatagc gctcgtcgcg aaaatcggga ttggcagcga gcaggatgcg ctggccgcgc
     1921 gtccacgatt cgagccggta cgcgccagtg ccgaccggat gctccatcac gcggtgcgac
     1981 tcgtcctggt agcgctcgac gacctcgcgc gcaacggcgg cgaacgacga cgtcgtgagc
     2041 caccacccga acgcgtaatc gggttccctg aagcggatgc gcagcgtgta gcggtcgatc
     2101 gcctgcaggc cgtcgatcgg ctcgtcgtag tcgaagcggc cgctcttgcg tgcgcgctcg
     2161 agcggtgcgt cgagcccgac gaggttgccc tcgaacaggt acagccagta cgagcgcagc
     2221 ttgggatcga acacgcgctt gatgctgtag acgtagtcgc cggcggtgag ctcgcgcggc
     2281 ttgccgccga acgccggatc ggcggcgaag cgaatgccgc gcttcacctt gatcgtgtac
     2341 gttcgtccgc cgtcgctcac ctgcggcagg ccgtcggcgg tgttcggctg cagcacgacc
     2401 ggtcgcgcga aatagtcgta gcgatagagc ggatcgaaaa tggcgagaca gacggcatcc
     2461 gagtacgtat cgctgatcgc ctgcggatcg aagccgcttt cggcgacggg aaaggcgacg
     2521 tgaagcgtct tgcgcatgtc ggcgccggca gccggaagca cgagtgcgag cgcgcaaacg
     2581 gcgataacca acgccttttt catcgtcgtc gaaccgaatt agggtcagag acgactttcc
     2641 ggttcgtgca agcccgtgtc tgaccgttca ggaagcgcgc gaggcgcgcg atgccatcgt
     2701 cgaggcgcga atcggcggcg gcgaagcacc agcggacgaa cccttcaccc tcggggccga
     2761 aggcgctgcc cggtgcgagc ccgagcttcg cttcctgcac cagccggcgg cagaacgcca
     2821 tgctgtcgtc cacaccttcg acgcgaaaga acgcgtacat cgcaccttcc ggtggcgcga
     2881 cttcgacgcc gggcagcgca cgcaatccgt cgacgagccg gttgcgcgcg cggcgcagcc
     2941 gctcgcgcgt cgcgtcgacc gtaggctcgc cgtcgcgaag ggcggccaca cccgcgcgct
     3001 ggacgaacac cggcgagcag gacgtgttgt actcgatcag cttgccgaga tcgggcatca
     3061 gcggcttcgg gcctacgatc cagccgaggc gccatccggt catcagccac gccttcgaga
     3121 agctgttcgt gctgacgacg cgcatgccgc cgtccgcgat gtcgagcagc gacggtgcgc
     3181 aggcgaaatc ttccgcgtag tagaggcgct cgtacacgtc gtcggcgacg atccagatgc
     3241 cgtggcgcgc gcagtgcgcg agcagcgcct gctgtgccgc gcggtcgatc gtccagccgg
     3301 tcgggttgtt cggtgaattg agcatcagcg cgcgcgtgcc gggcgtgagt gcgtcgagca
     3361 gccggtcgag gtcgaggcgc cagcccgacg cgccgaagga aagcggcacg cagacgacct
     3421 cggccgacag gatcttcgga atctcgacga gattcggcca gagcggcgtc acgacgacga
     3481 cgcggtcgcc gggcgccacc agcgcctcga ccgcaagcat cagcgcagac atgccggacg
     3541 cggtgacggc gatctcgtcg gccgtggtcg ggcggtgcag cgcgcccacg tagccggcga
     3601 tcgccgcgcg aagctccgga atgccgaagt tctgcgaata gaacgtctcg ccgcgcgcga
     3661 gcgacgccgt tgccgcgtcg cggatgaacg cgggggttac ctggtcgggc tcgccgaacc
     3721 agaacgcgag cacgtccggg tcgtgcatgc ccgcgttggc gatgtcacga atcttcgagc
     3781 cggccagtcc gcgcaccgcg gagcgggctt cggctaaccg ggacgggagg gagggcatca
     3841 gcccactata tcgcgatctc gtacctgtcc cgtccgtgcc ccttcgccgc gtaaagcgcg
     3901 ctgtcggcac gcttcagaag cgcctccggc gtcgtctcgc caccctggcc gagtgccacg
     3961 ccgatgctcg tcgtcacctt caccgccgcc tcgcccgcat cgaactcggg ccgcatcgcg
     4021 gcgatgatct tgcgcgcaac gaagcggcac tcctcgcgcg tgtgaacccc ttcgaggacg
     4081 atcacgaact cgtcgcccgc gagccgcgcg acgaggtcgg ttgcacgcac cgacgccgac
     4141 agccgtcgcg cgaactcgcg cagcacctcg tcgccggccg catgtcccag cgtgtcgttg
     4201 atctgcttga atccgtcgac gtcgagaaac agcagcgcaa ccggcgtcgc ggcgcgctgc
     4261 gagcggcgca acgcggcggc gatgccgtcg ttgaatgcgt gccggttggg cagcccggtg
     4321 agcgtgtcgc gctgcgcgag acgctcgagc tggcgctcga cctccttgag cggcgtgatg
     4381 tcggtcgacg acgtgtagac gccgagcacg gcgcccgacg cgtcgatgtc gggaatgtag
     4441 ctgccgcgga cgaaccggct gccctccggg ccgggcactt cgacgtcgaa gtcgacgcgc
     4501 tcgcccgcga acgcgcgatc gagattcggc gccacgttct cgtaggcacg cggcccgagc
     4561 acgtccgcca cgcgccgccc ggtaatctct gtcagcggcc gcccgagcca tgtctcgtag
     4621 tagcggttgt tgaagcggta gcaccggtcg caatcgatgt agccgaccat cgacggcatg
     4681 ctgtcggtca tgctgcgcag ccgctcttcg ctttcctcgg ccttcgcctg cgcggtacgc
     4741 aggtcggtga tgtcctgcac cagcgcatag agcccgagca cgcggccgct cgcttcctga
     4801 tcgggaatga gctcgaccag ctcgtggcag gcgcggccgt tacgcacgac cgtgcgctgg
     4861 aaccgcgccc gctcgccgcg gcacgcgcgc tcgagatgcg gtttcgactg cgcatacgtc
     4921 tcgggactca gcacgtcctg cgcacgctgc ccgagcatcg cctccggcga gagaccgaac
     4981 atctgctgcg agcgcgcatt gacgaagaga taacgctcgc cggcgtcgag gtaggcgatg
     5041 agcgcgggca ggttgtccgc caccgtgcgc atccgcttct cggacgcggc aagcgcctgc
     5101 ttggcctgcc gcaacgcggt gacgtcctgc gagatcgtga ccacgccgtg cacgactccg
     5161 gccgcatcga tgtcgggcac gtgatcgacc tggtagaact cggtgccgtt tgcgcgatcg
     5221 acgacgaggt cgaagcgccg cgcgagcccc gcgtagctgc gatcgagctc gggcttgatc
     5281 tgcgcgtacg cggcggagga caccacgtcg gcgagcgggc gcccgacgat atcggcgggt
     5341 tcgcggcccc agtattcggc aaagcgccga ttgccgaaga gcagccgatt gctcgtgtcg
     5401 atgtaggtga tccgcgcggg aatgccgtcg gcgatcagac gcacgaagcg ctcgcgtccg
     5461 gcgagcgcct cctcggcatc cttgcggcgc gtgatgtcct ggcacacgac gtaggcgccg
     5521 cgcacctcgt ggccgtcacc gtagtcgggg aagcaggtca ccatgaagac cgggccgcgg
     5581 ccgtcgccgg cgaaggtcgt ctcgaacgtc tgcggcatgc cggcgcgcac gtcgcgcatg
     5641 cgcgactcga ggcgcgcgta gaccgtcggg tgaagcaccg cgcgcaaccg atggccgacg
     5701 acgcgctcgg ggctcgcgcc gaacacgtcg cggtagtact cgttgacgaa ccggaagcgc
     5761 tcgcccgcat cgatgtaggc gatcagcacc ggtacgttgg ccgtgatcgt cgcgagctcg
     5821 ctcctgctgc gctcgagggc ggtcgtcttt tcctcgagcg cgccggtctt ctcctggagc
     5881 gcgcgcgtgc gctcctccac cgtttgcgcg agccggttga gcgtgcttgc cagcacgccg
     5941 gtttcgtcgt cgccgccaat cgacgtgcgg tgcgcaagat tgccgcgcgc cagcagcgtc
     6001 gcgtcgtagg cgagccggtg cagcggtcgc gcgatccgta cgccgagccg cgccgcgaag
     6061 ccgatcccga gcagcagcga cagcgcgccg agcgcgagcg tctcgaagcg gttggtgcgg
     6121 gcaatggaga gtgcggcatc cgccggcatg ccgacgaaga cgtgccatgg cgtccgcttc
     6181 gcttgcgtgt agccggcgat gcgcgggacg ttgtccgcac cgaccgtctc gtcgacgccc
     6241 tcgcggctgg cgaggtgctg gcgcgccttg ccgacggcga gcacgctcat gccgatccag
     6301 cggtccgggt cgacgctccg cgcgagcacg acgccgtccg cattcacgat cgagatgacc
     6361 gttcctcgcg gcaccgcgcc gccgaggtcg aggtgtcgct gcacctgctt gagatcgatg
     6421 gcaagcgtga cgacgccggc gacggcgccc gtcgcgccga tcacggggcg ggcgaacgcg
     6481 acgacgggat tgccgtcgtc gtccgacgcg agcggacctt cgatcgcggt cttcgacgtc
     6541 tgcaccgcgc gccggaaata atcgcgatcg gcgatcgaac ggccggcgtt cgcactcgta
     6601 cggttcagca tgccgacatt gcgtccgtcg agcgtccaga cgccgacgtt ctgcaccatc
     6661 ccggggaggt cgccgcgaat cttgtcgagg aatgcgtcgt tggcggaggt ggcggacggg
     6721 tcgagcgtca cgccgtggcg caccagcgcg agtgcgatga ggttgtcacc tacaacgtcg
     6781 tcgacccgcg cggcgacgac gcgcgcaagc gccagcgtgc gctgctgcgc aaactcgcgg
     6841 gcggtcgatg cctgcctggc ggcattgacg ccgacgtaaa cgacgaacgg aagcccgagt
     6901 gcgagtgcca acagcgcgag cctgagcccg atcgacgagc gcaggcggac gaggcctcgc
     6961 ccgcgcagca accgcgcgac gcgcaaccgc cagccggcgc tgcgccactc ccttggggaa
     7021 gcggcggcgg tgacgcgctg cgtcgaattg tcgtccggca tggcgagcgt aaggctgttc
     7081 gaggtcccgg tggatgacgc atcggcgttt ccgcaaggag catgccgatg tcattgggcg
     7141 ataatgcccg gcccttttcg ccgatcgccc gacgctcccc gccgatgaac cccgtctcgc
     7201 tcgccctgca tcccgcggtt gtctgcgcgg cgcttctgtc ctgctcgaac ctgttcatga
     7261 cgttcgcgtg gtacgggcat ttgaagctcc tcgaaaaccg cgcctggtgg gtggccgcgc
     7321 tgatttcgtg ggggatcgcg ctcttcgaat acctgctgca ggtgccggcc aaccggatcg
     7381 gcttcacggt gctgtcgctc gcgcaactca agatcatgca ggaggtgatc acgctcaccg
     7441 tgttcgttcc gttcgcgatc tttttcatgg gcgcgacgct gaagctcgac tacctgtggg
     7501 ccggcctttg catgatgggc gccgtctatt tcatcttccg cgggtagggc gcgcccgcgc
     7561 gcccggcacg aatcgcatgc atctgcacat cctcggcatc tgcggcacgt tcatgggcgg
     7621 catcgcggcg atcgcgcggc gtgcgggcca cacggtcacc ggctgcgacg ccaacgtcta
     7681 cccgccgatg tcgacgcagc tcgcagcact cggcatcgcg ctcaccgagg gcttcgacgc
     7741 cggccagctc gacgccgtgg cgaggcgcgc cgacgtgttc gtcgtcggca acgtcgtgtc
     7801 gcgcggcaac ccgctgatgg aagcgatcat gaacgcgggc cgcccctacg tttcgggacc
     7861 gcggtggctg ttcgaaaacg tcttgcgagg ccgcttcgtg ctcgcggtcg cgggcacaca
     7921 cgggaagacg acgacgtcgg cgatgcttgc gcacatcctg gaggcggccg gcctcacccc
     7981 ggcgttcctg atcggcggcg tcccggtcga cttcggcgtg tccgcgcggc tcaccgacag
     8041 cgcgttcttc gtgatcgagg ccgacgagta cgacaccgcg ttcttcgaca agcgatcgaa
     8101 gttcgtccat taccggccac gcactgccat cctcaacaat ctggaattcg atcacgccga
     8161 catctttccc gacctcgctg cgatcgagac gcagttccat catctgctgc gcacgattcc
     8221 cggggatggc cgcatcgtcg tcaatgccgg tgacaatgcg ctggcgcgcg tgatcgcgcg
     8281 cggtgcctgg acgccgatcg agcgcttcga cacggcggca tccaccgccg cgcccgcacc
     8341 cgactggacg atcgccgacg cgggtggcgt gacgcttggc ggcgagacga tcggggtcct
     8401 cgatttcgcg cagtcgggac gccacaaccg gctgaacgcg ctcgccgcaa tcgctgcggc
     8461 gcgtcatgcc ggcgtaccgc cgcacgatgc actcgccgcg ctgcgctcgt ttcgcggcgt
     8521 gcgccggcgc atggagctgc gcggtcacgc cggcggcgtc gcagtgtatg acgattttgc
     8581 gcaccacccg acggcgatcg ccacgacgct cgacggcctg cgcgcgcggc aggcgcaggg
     8641 gcgcatcctc gccgtgctcg agccgcgctc caacacgatg aagctcggga cgatgaaggc
     8701 ggcgctgccg gccagcctcg cagcggcaga ccgcgtgtac tgctatggcg cgaatctcgg
     8761 ctgggatgcc gccgccgcgc tcgcaccgct cggcgcccgc gcggaggtcc acgacgacct
     8821 tcgcgcgctc gtggcggccg tggcacgcga cgcgcgcgcc ggcgatcatg tgctcgtgat
     8881 gagcaacggt ggcttcggcg gcgtgcacga gaagcttctc gacgcgctgg cggcgcgcgc
     8941 cgacccggcc tgatggggcc ggtgcgcccg acgatcgtct acctgcacgg ctttcgctcg
     9001 tcaccggcga gcgccaaggc agcgcgcctt gccgaggcgg ttggccggct tccggaacgc
     9061 gatcgcccgg tgctcctcat tccgcaactc aacccccgcc cggcgcttgc catggccgcg
     9121 atcctcgccc tgactcgcga cgccgatccc tgcggcgtga cgttcgtcgg cagctcgctc
     9181 ggcggctact acgcgacgta cgccgcggag acgctcggcg cccgggcggt actcgtcaat
     9241 ccggcagtgc gcccgtatga cgacctcgtg ccgtaccgcg gccggcaggt gaacctctac
     9301 acgggcatcg cgttcgacgt gaccgagcgc catttcgagg agctgcgcgc gctggcgatc
     9361 ccgaagatca cgcggccggc gcgctacctg ctgctcgtgc aatcgggcga cgaggtgctc
     9421 gactaccggg tcgcggtcgc cttctacgcc agcggatggc agcaggtcac cggcggcggc
     9481 gaccatgcgt tcagcggctt cgagacgcac atcccgggaa tcctgcgctt ttgcggcgtc
     9541 ggcagcgccc ccggctttgt gcggcgcgac tagcgacaaa gtgcaggcgc gcgttatgct
     9601 tgatatccgc tgcgccgcgg ccacaagccg cggccgtacc cgctgcccgc gcacgatctc
     9661 ggcctgaccg gcccgttgtg cggtcggttt cgctcgacag gagcttgcga tggcaactca
     9721 cgaaaacaac gatcttcgtg cttcgtactc cgctgcgtca cgccgcccgc cggccgcgcg
     9781 cgcgggccgc gacccggcgt cgcagccact gtccgatgcc gcactcgacc tgatcggtcg
     9841 cgaaaaagcg ttcggcgcac gcaattacga tccgctgccc gtcgttctcg cgcgcggcga
     9901 aggaagctgg ctcgccgacg tcgacggtca ccgctacctc gacctgatga gcgcctactc
     9961 ggcggtcagc ttcggccacg cccatccgcg catcctcgcc gcgctggtcg cgcaggcgca
    10021 gcggctcggc gtcacgtcgc gcgcctacta caacgacgag ctgccgggcc tgctcgagcg
    10081 cctcgccctg ctcaccggtc tcgaccgggc gctgccggcg aacggcggcg cggaggcggt
    10141 cgaaacggcg atcaaggcgg cacgcaagtg gggcagcaag gtcaagggca tcgccgaagg
    10201 ccgcgtcgag atcatcggct gcgacggcaa ctttcacggc cgcaccatcg ccgcgatcgg
    10261 cctgtcgacg gagccgcagt accgcgacgg cttcgggccg ttccctcccg gcttgtcgac
    10321 catcccctac ggcgacgccg atgcgctcgc gcgtgcgatc acgccgcgca ccgccgcatt
    10381 cctcgtcgag cccatccagg gcgagggcgg tatcgtcgtg ccgccgcccc gctatctggc
    10441 gcgctgcctg cagatctgcc gcgatcacca cgtgctgctg atctgcgacg aaatccagac
    10501 cggcctcgga cgcaccggac gctttctcgc ctcggagcac gacggcgtga agcccgatgg
    10561 cgtcatcctc ggcaaggcgc tcggcggcgg gctgctgccc gtctcggcgt tcgtcgcaac
    10621 gaacgacgtc atgcaggtgt tccatccggg cgaccatggc agcacgttcg gcggcaaccc
    10681 gcttgcgtcc gccgttgcga tggccgcgct cgacgtgctg ttcgacgaga agctggtcga
    10741 gcggtcggcc gagctcggcg catggctctt gtccgaattg caggatctgc gcagccacgt
    10801 cgtgtccgac gtgcgcggac gcgggctgtt cgtcggcatc gaggtcgatg cccgttacac
    10861 gagcgcccgc ccggtcgtcg accggctgct tgcgcgcggc atcctgtcga aggacaccca
    10921 cggcaccgtc gttcgcatcg cgccgccgct caacatcgcg cgcgacgatc tcgcctgggc
    10981 gatcggcgaa atccacacgg tatttcgcga gctcgaacgc gacttgaagc gcgccgcata
    11041 gtcgcctgca ccctctgtcg ggcggggctg caataatccg ggcacggcgc gaaagcgcgc
    11101 cgtcgcctta gcactcgcct cgctgccatg cgttgccttc gcctccttgc cttcctgacc
    11161 ggcgtcttcg ccgcctgcgg ccttacgacc ggcgcttacg ccgtgtcgcg catctgcatg
    11221 agcgccgact cgctgtcctt tggacagcga ccggtcggca gcagcgagtc ggcgacggtt
    11281 gtcgtttcga actgcggcga cgcgccgttc gccttcaccg acgtaagccg acatacggcg
    11341 acgaacgtgg cgtaccggac gcaaacttcc tgctcgaccg gcatgacgct cgctcccggc
    11401 gcccaatgcg cggccagcgt ctacttcgag ccgacggtcc cgggacaggc gtcgggcgcg
    11461 ctctggttcc acaacaccac gtcgacgccc gaccagttgc tgacgttcta cggccggggc
    11521 gtggacgcgc aggcggggac ggcgtcgctc gtcttttcgc cggcgatcgc gaatttcggc
    11581 gacgcggccg tcggacgcga aacggcgccg ctcgtgctga cgctgcaaaa cgccggcacg
    11641 gcaccgctgg tgccgagtgc gctcgtgctc aatggccgcg atccttacga ttttcgcggc
    11701 gagaccggca ccggtgcgtc gtcctgcggc atcggccgcg cgattgcgcc gggtgctggc
    11761 tgcacgctga tcctctactt ccatccgcag gcgagcggtg cacggcaggc gaacctcgtc
    11821 gtcgatgcgc cgcagcttgc ggcactcgct gtcgtcacgc ttgccggaga tggcatggac
    11881 gca
//
LOCUS       DASSVX010000347         4473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1282037, whole genome shotgun sequence.
ACCESSION   DASSVX010000347 DASSVX010000000
VERSION     DASSVX010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282037"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="VFO53_14865"
     CDS             <1..1422
                     /locus_tag="VFO53_14865"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HET8877608.1"
                     /translation="VVEPCSNGIGSDLFAIVWDGERLAGLNASGRAPAGWNRARFAGH
                     AQMPQRGFDTVTIPGAVSGWVALSQRYGKLAFGDLFEPAIRYARDGYAVSPVVADKWR
                     LAVPLMPQGLGWQDHFLVDGRAPNPGERFRSSAMANTLSAVAQTNGEAFYRGALADAI
                     CAHAKACGSVHVRRDFERHTSDWVTPIAVDYRGMRVHEIPPNGQGIAALMALGMLESF
                     DLASLAPDSVESMHLQIEAMKLAFADVHRYVGDPATMTISPEALVERDYLRQRASLID
                     ASKANHYGPGEPPRGGTVYVTAADASGMMVSLIQSNYMGFGSGVVVPGTGISLQNRGA
                     GFTLAHGHANEVGAGKRPYHTIIPGFMTKDDAPVATFGVMGGPIQPPGHVQTVVRMID
                     YAMNPQATLDAPRFKVNVGRSIDLEASAPRELRDGLIALGHKLEAIPDSYMDFGAGQV
                     IQKADVGYVAASDPRRDGQAAGF"
     gene            complement(1501..2109)
                     /locus_tag="VFO53_14870"
     CDS             complement(1501..2109)
                     /locus_tag="VFO53_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HET8877609.1"
                     /translation="MIVFAINGVTPVVDPTAYVHPSAVLIGDVIVGAGCYVGPCASLR
                     GDFGRIEVRAGANLQDACVAHGFPGTDTIVAEEGHIGHGAILHGCVVERNALVGMNAV
                     INDNAVIGESAIVAAMAFVKAKMVVPPRTLVAGMPARLVRELTETELAWKIEGTRSYQ
                     ELTRRSLATMRATAPLTAVEPDRQRIELPELLPLSSVKAKRT"
     gene            complement(2130..2546)
                     /locus_tag="VFO53_14875"
     CDS             complement(2130..2546)
                     /locus_tag="VFO53_14875"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET8877610.1"
                     /translation="MIVYADSSVLLRLVLGQTDALAEWKQVGVAISSALAEVECLRTL
                     DRLRLADDLSDEVLAARRQAIYDLIESIELAALTSSVLARAAQPFPTALRTLDAIHLA
                     TALLWKEHRGADLVMATHDRALAIGARAMGLQVVGA"
     gene            complement(2543..2803)
                     /locus_tag="VFO53_14880"
     CDS             complement(2543..2803)
                     /locus_tag="VFO53_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HET8877611.1"
                     /translation="MDSVGIAELKSRLSEYLRVVRSGRSLTILDRETPIARIVPWKRD
                     DPLQARKPRPGAPRPSHVPLPPPLPYRGDIVDLLLEERRDRL"
     gene            complement(2859..>4473)
                     /gene="purL"
                     /locus_tag="VFO53_14885"
                     /gene_synonym="purI"
     CDS             complement(2859..>4473)
                     /gene="purL"
                     /locus_tag="VFO53_14885"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET8877612.1"
                     /translation="DTVRAVALDFCVPLGLSIPVGKDSLSMRTTWRDGDVDKSVTAPV
                     SLIVSAFASIADARACLTPLLRMDRGATALVHLDIASARRRLGASALAQVYGQLGDDA
                     PDVEASRLAAFFSLVQRLHADGRLLAYHDVSDGGLVATLCEMAFAARCGLDVGLPVAA
                     DAALAALFAEELGAVVQVRAGDMDAVLVRARDAGVAATAIGTASRGDRVVIRCCDRVV
                     LDEPRIELHRAWSATTHAMQRLRDNPQAADDEYARLLDDEPGIVPALTFDVHDDPAAP
                     FIATGVRPRVAVLREQGVNGHVEMAAALTRAGFDAYDVHMTDLAAGRHALGDYVGVVA
                     GGGFSYGDVLGAGEGWAKSILFDPRRRDAFAAWFARTDTFALGVCNGCQMMSNLSEII
                     PGAAHWPHFVRNASEQFEARYVMLEVQRSPSLFFDEMAGSRIPVALAHGEGYTEFRDD
                     TQLAAAQPYVALRFVDHRGRATERYPYNPSGSPQGITGLTTPDGRFTILMPHPERVFR
                     TVQMSWHPDDWGELSPWYRMFANARRFVG"
BASE COUNT          767 a         1507 c         1511 g          688 t
ORIGIN      
        1 gtcgtcgagc cgtgcagcaa cgggatcggt tccgacctct tcgcgatcgt ctgggatggc
       61 gagcgcctcg ccggtctcaa tgcatcgggc cgcgcaccgg ccggctggaa tcgcgcgcgc
      121 ttcgccgggc acgcgcagat gccgcagcgc ggcttcgata cggtgaccat tccaggcgcg
      181 gtgtccggct gggtcgcgtt gtcgcagcgc tacggcaagc tcgcgttcgg cgatctgttc
      241 gagccggcga ttcgctacgc acgcgacggc tacgcggtgt cgccggtggt tgccgacaag
      301 tggcgtctcg ccgttccgct gatgccgcag ggcctcggct ggcaggatca cttcctggtc
      361 gacgggcgcg caccgaatcc cggtgagcgc tttcggtcat cggcgatggc gaacacgctg
      421 tcggcagtcg cgcagacgaa tggcgaggcg ttttatcgcg gcgcgcttgc cgacgcaatc
      481 tgcgcgcatg cgaaggcgtg cggcagcgtg cacgtgcgtc gcgatttcga gcggcacacc
      541 agcgactggg tgacgccaat tgccgtcgac tatcgcggca tgcgcgtgca cgagattccc
      601 ccgaacggac aaggcatcgc cgcgctgatg gcgctcggca tgctcgaatc gttcgatctc
      661 gcatcgcttg cgcccgattc ggtcgagagc atgcacctgc agatcgaagc gatgaagctc
      721 gcattcgccg acgtgcaccg ttacgtcggc gatccggcga cgatgacgat ctcgcccgaa
      781 gcgctcgtcg agcgcgatta tcttcgccag cgcgcgtcgc tgatcgacgc gagcaaggca
      841 aatcactatg ggcccggtga gccgccgcgc ggcgggaccg tgtacgtcac cgcggcggac
      901 gcgagcggca tgatggtgtc gctgatccag tcgaactaca tgggcttcgg ctcgggtgtc
      961 gtcgtgccgg gtaccggcat cagcctgcag aaccgggggg ccgggttcac gcttgcgcac
     1021 ggtcacgcga acgaggtggg cgcgggcaag cggccgtacc acacgatcat tcccggcttc
     1081 atgaccaagg acgacgcgcc ggttgcaacg tttggcgtga tgggcggccc gatccagccg
     1141 cccggccacg tgcagaccgt cgtgcggatg atcgactacg cgatgaatcc gcaggcgacg
     1201 ctcgacgcgc cgcgcttcaa ggtgaacgtg ggccgctcga tcgacctcga ggcgagcgca
     1261 ccacgcgaat tgcgcgacgg tctcatcgca ctcggccaca agctcgaggc gattcccgac
     1321 tcgtacatgg acttcggtgc gggacaggta atacagaagg ccgacgtcgg ctacgtcgcc
     1381 gcgtcggacc cgcgccgcga tgggcaggcg gcggggttct gaggcgagcg ccgagccgca
     1441 gttgcgcgct tcccgcgtga cggcgcttcc cgcgtgacga cgctccccgg gtgacagcgc
     1501 ttacgtgcgc tttgctttca ccgacgacag cggcagcagt tccggcagct cgatgcgctg
     1561 gcgatcgggc tcgaccgccg tgagcggcgc cgtcgcacgc atcgtcgcaa ggctgcggcg
     1621 cgtcagttcc tgatagctgc gcgtgccctc gatcttccac gcgagctcgg tctcggtcag
     1681 ctcgcggacg agccgcgccg gcatgcccgc gacgagtgtg cgcggcggca cgaccatctt
     1741 cgccttgacg aacgccatgg cggcgacgat ggccgattca ccgatcaccg cgttgtcgtt
     1801 gatcaccgcg ttcatgccga cgagggcgtt cctctcgacg acgcagccgt gcaggatcgc
     1861 gccgtggccg atgtgtcctt cctccgcgac gatcgtgtcg gtgccgggaa agccgtgcgc
     1921 gacgcacgca tcctgcaggt tggcgccggc gcgcacctcg atgcggccga aatcgccacg
     1981 caacgacgcg cacgggccga cgtagcaccc agcgccaacg atgacgtcgc cgatcaggac
     2041 cgccgacgga tgcacgtagg cggtgggatc gacgaccggc gtgacgccgt tgatcgcaaa
     2101 aacgatcaag ctcagttcgc tgcgggaagt cacgcgccca cgacctgcag tcccatcgcc
     2161 cgtgctccga ttgccagcgc ccgatcatgg gtcgccatca ccaggtcggc accacgatgc
     2221 tctttccaca acagcgcggt cgcgaggtga atggcgtcga gcgtgcgaag ggccgtcggg
     2281 aagggctgcg cagcgcgggc gagaaccgat gaggtcaagg cggcaagctc aatcgattcg
     2341 atcagatcgt agatggcctg tctgcgcgca gccaacacct cgtcggaaag atcgtctgcc
     2401 agccgcaggc gatcgagagt gcgcaagcac tcgacttcgg caagcgcaga tgagatcgca
     2461 acgccgacct gcttccattc ggcgagggca tcggtctgac ccagcacgag tcgcagcaac
     2521 acggacgagt cggcgtagac aatcacaagc gatcccgccg ctcctcgagc aggagatcga
     2581 cgatgtctcc gcgatacggc aacggcggcg gcaacggcac gtgcgacggc ctcggggcgc
     2641 ccggacgtgg ctttctggct tggagagggt cgtcacgctt ccatggcacg atccgcgcga
     2701 tgggcgtttc gcgatcgagg atcgtcagcg agcggccact gcgcaccacg cgcaggtact
     2761 cgctcaaccg gcttttcaac tcggcgatgc caacggagtc catggtcaca tgctagccac
     2821 ttctagttag tttagcaagc ggccgggtgg ggagacgctt accccacgaa gcgtcgtgcg
     2881 ttcgcgaaca tgcgatacca cggcgacagc tcgccccagt cgtcagggtg ccacgacatc
     2941 tgcaccgtgc gaaatacgcg ctccggatgc ggcatcagga tggtgaatcg cccgtcgggc
     3001 gtggtgagtc cggtgatccc ttgcggcgac ccgctcggat tgtacggata gcgctccgtc
     3061 gcacggccgc gatgatcgac gaagcgcagc gcaacatacg gttgcgccgc ggcgagttgc
     3121 gtatcgtcgc ggaattcggt gtagccctcg ccgtgcgcga gtgcgacggg aatgcgactg
     3181 cccgccattt cgtcgaagaa gagcgacggc gagcgctgaa cctcgagcat cacgtagcgc
     3241 gcctcgaact gctcggaggc gttgcgcacg aagtgcggcc agtgcgccgc accgggaatg
     3301 atctcgctca ggttgctcat catctggcat ccgttgcata cgccgagcgc gaacgtgtcg
     3361 gtgcgcgcga accaggccgc gaaggcgtcg cgccggcgcg gatcgaaaag tatcgacttc
     3421 gcccaaccct cgcccgcacc gagcacgtcg ccgtacgaaa aaccgccgcc cgcgacgacg
     3481 ccgacgtaat cgccgagcgc gtggcggccc gccgcgagat cggtcatgtg cacgtcgtac
     3541 gcatcgaagc ccgcgcgcgt gagcgccgcc gccatctcga cgtggccgtt cacgccctgc
     3601 tcgcgcagga cggccacgcg gggccgcacg cccgttgcaa tgaacggcgc ggcaggatcg
     3661 tcatgcacgt cgaacgtgag cgcgggcacg atccccggct cgtcgtccag caagcgcgcg
     3721 tattcgtcgt cggcggcctg cgggttgtcg cgcaaccgct gcattgcatg cgtcgtcgcc
     3781 gaccatgcac ggtgcagctc gatgcgcggc tcgtcgagga cgacgcgatc gcaacagcgg
     3841 atcaccacgc gatcgccgcg cgacgccgtg ccgatcgcgg tcgcggcaac gcccgcgtcg
     3901 cgtgcccgca cgaggaccgc atccatatcg cctgcgcgaa cctgcacaac tgcgccgagc
     3961 tcttcggcga acagtgcagc gagcgccgca tcggctgcca ccggcagccc aacatccagg
     4021 ccgcagcgtg ccgcaaacgc catttcgcac agcgtcgcga ccaacccgcc atcgctgacg
     4081 tcgtggtagg cgaggagccg gccgtcggcg tgcagccgtt gcaccagcga aaagaatgcg
     4141 gcgagtcggg acgcctcgac atcgggcgcg tcgtcgccga gctggccgta cacctgggca
     4201 agcgccgacg cgccaagacg ccggcgggcg gacgcgatgt cgagatgcac gagcgccgtg
     4261 gcgccacgat ccatgcgcaa cagcggcgtg aggcaggcgc gggcatcggc gatcgatgcg
     4321 aacgccgaaa cgatcagcga gaccggtgcc gtcacgctct tgtcgacatc tccgtcgcgc
     4381 caggtcgtgc gcatcgacag cgaatccttg cccacgggaa tcgacagtcc cagcgggacg
     4441 cagaagtcga gcgcaactgc gcgcaccgtg tcg
//
LOCUS       DASSVX010000348         6004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_481977, whole genome shotgun sequence.
ACCESSION   DASSVX010000348 DASSVX010000000
VERSION     DASSVX010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481977"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="VFO53_14890"
     CDS             <1..1435
                     /locus_tag="VFO53_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HET8877613.1"
                     /translation="ARFPVYRTYVDANGPSAQDRHHIGWAVAHARAHSRVSDVSAFAF
                     LHRALLGTPAAGDESAAPAYRDFAMRFQQFTAPVAAKGVEDTALYRFQRLVSLNEVGG
                     DPEQFGISINAFHAASADRAAVWPHTMLATSTHDNKRSEDVRARIDVISEMPAAWRTM
                     VRRWSRMNRSRKRLVDGEAAPSRSDEYLLYQTLAGTFAAARDAGDDLAAYRERIAAYM
                     RKAAREAKLHTSWLAPNEAYENALRDFVSTLLGEESGQRFLADLRAECATFAWFGLLN
                     SVSMTLLKLASPGVPDIYQGNELVDLSLVDPDNRRPVDYPLRRERLASLASLATAPRD
                     ARAARLRALFAAPFDGMAKLWVTARALALRRQAPALFAFGDYRPIGVQGAHADRIVAF
                     ARVHERRGAVVVVAGRLFASLGLPAGTLPLAGAWGDTTLDLSFVAPGAKLDDVLSGEA
                     VIATGNPMPVSQVLADFPGALLHCTVD"
     gene            1446..2534
                     /gene="cax"
                     /locus_tag="VFO53_14895"
     CDS             1446..2534
                     /gene="cax"
                     /locus_tag="VFO53_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HET8877614.1"
                     /translation="MGISAWLLVLVPIALGVHFLGDLGPLWTFAAAIVAIVPLAEWIR
                     RGTEQIARRAGSAVGGLLSATFGNTAELVLAIFVLLAGQADVVKAQITGSIIGNGLLG
                     FGLAITVGTWGRRELRFKPERAGLLSSLLMLVTIALLVPALFNYTERGIFASTQASSL
                     DEKLSLCVAVVLIVIYAANLVYTLVTHRDVFATDEPGDDRKPAWPLWRAIAVLVAATA
                     ATAWMAELVSGALGATARTIGVSTFFLGVVVLAIIGNAAEYFSSIYFARQGRLGLVAT
                     ITVGSTIQVALLVAPILVLVSHLTGHPMNLVFDNPLELVAIASVAFVVNAITRDGEAT
                     WFEGVLLIGVYVLLAFAFFFATPPPAPA"
     gene            complement(2563..3000)
                     /locus_tag="VFO53_14900"
     CDS             complement(2563..3000)
                     /locus_tag="VFO53_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877615.1"
                     /translation="MPGPGDSGSLEFEKVSLSDARKALEETGPAAYSVAKQPENWVEK
                     RTPTPDETLSDEANAWMAALPESVRPRQLALRYARLANRLAQLWSDPVKCERLLDDLM
                     TDHRGGRKGFVLQIASELATLRDHYYREHHGGKSPWEYVETGR"
     gene            complement(3329..3532)
                     /gene="cspD"
                     /locus_tag="VFO53_14905"
     CDS             complement(3329..3532)
                     /gene="cspD"
                     /locus_tag="VFO53_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008156 - negative regulation of DNA
                     replication [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HET8877616.1"
                     /translation="MATGTVKWFNDAKGFGFITPDGGGEDLFAHFSAINMQGFKTLKE
                     GQKVSFEVTQGPKGKQASNIQAV"
     gene            complement(3799..4824)
                     /locus_tag="VFO53_14910"
     CDS             complement(3799..4824)
                     /locus_tag="VFO53_14910"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014292438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET8877617.1"
                     /translation="MTLLRTTIAGSLPKPSWLAEPEALWAAWKLDGEALAEGKRDAVR
                     LVLRDQEQAGIDVVTDGEQTRRHFVTTFIEGLDGVDFEHRKTVRIRNRYDASVPVVVG
                     PVARRHPIYVDDAAFLRAETDRPVKYTLPGPMTMVDTLSDAHYGSREKLAWAFAEILN
                     QEARAIAAAGVDVIQFDEPAFNVYFDEVRDWGVATLERAAEGLQCTKAVHICYGYGIK
                     ANIDWKNSLGGEWRQYEQTFPRLAASTIDQVSLECAHSHVPIELIGLLEGKDVLVGAI
                     DVASDRIETPAEVAEVIRSALRFVPADRLLPCTNCGMVPLARPVARQKLRALGEGAAI
                     VRRELTS"
     gene            4967..5944
                     /locus_tag="VFO53_14915"
     CDS             4967..5944
                     /locus_tag="VFO53_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HET8877618.1"
                     /translation="MSRILFADNDFPDVDLERDIFAGANLSLELAQCRSEAEVVAAAR
                     GCVAILLQYAPITAGVVAASPSLGIVSRIGAGFDTVDTAACEAHGVWVANSPDYGIGE
                     VATHALALALASIRNVARYHDDVRAGRWHYLSGGTLRRPASLTLGIVGLGRIGKRMAH
                     VAHNVVKRVIAYDPLLIDGDFPAYVERAPSLPELAAQSDIVSLHTPLDASTRGMIDKR
                     FFAAARRDMTLVNTSRGGVVDIADLLAALDAGILRGAALDVLPEEPVAPDSPLRADPR
                     IILTPHAAFYSIEAAQELRRKAAQNIVTWWRTGRPDYPVVRGTRKPPGT"
BASE COUNT          894 a         2032 c         2015 g         1063 t
ORIGIN      
        1 cgcgcgcttt ccggtctatc gcacctacgt cgacgcgaac ggtccgtcgg cgcaggaccg
       61 ccaccatatc ggctgggccg tcgcgcatgc tcgcgcgcac agccgggtga gcgatgtcag
      121 cgccttcgcc ttcctgcacc gcgcgctgct cggcacgccg gcagccggtg acgaatccgc
      181 ggcacctgcc taccgcgact ttgcgatgcg cttccagcag ttcaccgcgc cggtggcggc
      241 gaagggcgtc gaggatacgg cactctaccg atttcagcgc ctcgtctcgc tcaacgaggt
      301 gggcggcgat cccgagcagt tcggcatcag catcaacgcg ttccatgcgg caagcgccga
      361 ccgcgcggcg gtctggccgc acacgatgct cgccacgtcg acgcacgaca acaagcggtc
      421 cgaagacgtg cgtgcgcgca tcgacgtgat ctccgaaatg ccggccgcct ggcgcacgat
      481 ggtccgccgc tggagccgca tgaatcgcag ccgcaagcgc ctcgtcgacg gcgaagcggc
      541 gccgtcgcga agcgacgagt acctgctgta ccagacgctg gccggaacct ttgccgcggc
      601 tcgcgacgcc ggagacgatc tcgcggcgta ccgcgagcgg atcgccgcgt acatgcggaa
      661 ggcggcgcgc gaggcaaagc tgcacacgag ctggctcgcg cccaacgaag cctacgagaa
      721 cgccctccgg gattttgttt cgacgctcct cggtgaggaa agcggtcagc gcttcctcgc
      781 cgaccttcgc gccgaatgcg cgacgttcgc gtggttcggc ctgctgaaca gcgtgtcgat
      841 gacgctcttg aagcttgcct cgcccggcgt gcccgacatc taccagggca acgagctcgt
      901 tgatctttcg ctggtcgacc ccgacaaccg ccggccggtc gattatccgc tgcgccggga
      961 gcgtcttgcc tcgctcgcat cactcgccac cgcgccccgc gacgcacggg cggcgcggct
     1021 gcgggcgctc ttcgcggcgc cgttcgacgg catggcgaag ctgtgggtga ccgcgcgtgc
     1081 gctcgcgctg cgccggcagg cgccggcgct gttcgcgttc ggcgactatc ggccgatcgg
     1141 cgtgcagggc gcacatgccg atcgcatcgt ggcgttcgca cgcgtgcacg agcgccgggg
     1201 tgccgtggtg gtggtggcgg gccggctgtt cgcgtcgctc gggctcccgg cgggcacatt
     1261 gccgctcgcc ggcgcgtggg gcgacacgac gctcgacctg tcgttcgtcg cgcctggtgc
     1321 gaaactcgac gacgtgctga gcggcgaggc tgtcatcgcg acgggcaatc cgatgcctgt
     1381 gtcgcaggtg cttgccgact tccccggcgc gctgcttcat tgcacggtgg attgacgccg
     1441 acccgatggg catttccgcc tggctgctcg tgctggtgcc gattgcgctc ggcgtgcact
     1501 tcctggggga tttgggaccg ctgtggacat tcgccgccgc cattgtcgcg atcgttccgc
     1561 tggccgagtg gatccgccgc ggcaccgagc agatcgcgcg gcgcgccgga tccgcggtcg
     1621 gcggcctgct gagcgccacg ttcggcaata ccgccgagct cgtgctggcg attttcgtgc
     1681 tgctcgccgg acaggcggac gtcgtcaagg cgcagatcac cggctcgatc atcggcaacg
     1741 gcctgctcgg tttcgggctc gcgatcacgg tcggcacgtg gggccggcgc gagctgcgct
     1801 tcaagcccga gcgcgcgggt ctgttatcga gcctgctgat gctcgtcacg atcgcgctcc
     1861 tcgtgccggc gcttttcaat tacacggagc ggggaatctt cgcgtcgacg caggcgtcgt
     1921 cgctcgacga gaagctgagc ctttgcgtgg cggtcgtgct gatcgtcatc tacgccgcca
     1981 acctcgtcta cacgctcgtc acgcaccgcg acgtgttcgc aaccgacgag ccgggggacg
     2041 atcgcaagcc cgcgtggccg ctatggcggg cgatcgcggt gctcgtcgcg gcgaccgcgg
     2101 ccaccgcatg gatggcggag ctcgtgtccg gggcgctcgg cgcgacggca aggacgatcg
     2161 gcgtgtccac gttcttcctc ggggtcgtcg tgctcgcgat catcggcaat gcggccgagt
     2221 acttctcgtc gatctacttc gcgcgtcagg ggcgcctcgg actcgtcgcg acgatcaccg
     2281 tcggatcgac gatccaggtg gcactgctgg tggcgccgat cctggtgctc gtgtcgcacc
     2341 tgaccggcca cccgatgaac ctcgtcttcg acaatccgct cgagctggtc gcgattgcaa
     2401 gcgtcgcgtt cgtcgtcaat gcgatcacgc gtgacggcga agcgacgtgg ttcgaagggg
     2461 tgctgctgat cggcgtctac gtgctgctcg cattcgcctt cttcttcgcg acgccgccgc
     2521 cggcgccggc ctgaagcgcg acgcgcacct cgcgcggcag tctcagcgtc cggtttcgac
     2581 gtactcccac ggcgatttgc caccgtgatg ctcgcggtag tagtgatcgc gcagcgtggc
     2641 aagctcgctc gcgatctgca gcacaaagcc cttgcgcccg ccgcggtggt cggtcatgag
     2701 gtcgtcgagc aggcgttcgc atttgacggg atcgctccac agctgcgcca gccgattcgc
     2761 gagccgcgcg tagcgcagcg ccaactggcg cggacgtacc gactcgggca gtgcggccat
     2821 ccatgcgttc gcctcgtcgg agagcgtctc gtcgggcgtc ggggtgcgct tttccaccca
     2881 gttctcgggc tgcttcgcga ccgaatacgc ggcgggaccg gtctcttcga gcgccttgcg
     2941 cgcgtcgctc agcgacacct tttcgaactc aagcgaacct gaatcgccgg gaccgggcat
     3001 gacgcgcgct ccttcttgtc gcaatcgtcg tcgatggcgc acgctcgtgc acgcggtagc
     3061 ctgcgccagt ctctggcccg cagtataccg gcgcgattgc atgcgtcggc ggacaggacc
     3121 gcaaccggcg gccccggctg catcctaatt gcggcagcgt acgcgcgcga gcggcggcgt
     3181 gcgcggcggt gcgaacgcac cgacgaacgg cgatgcgttg attggattac tctgcacgct
     3241 gcggggccga ttcgcgcttg cgacggcccg gcgccaagaa aaaaggccgg gcagcgcccg
     3301 gccttcggtg aagcgggaag cgagacgatc agaccgcctg gatgttcgaa gcctgcttgc
     3361 ccttcgggcc ctgcgtcact tcgaagctga ctttctggcc ttccttgagc gtcttgaagc
     3421 cctgcatgtt gatcgccgag aagtgtgcga acagatcttc accaccacca tcgggagtga
     3481 tgaatccgaa gcccttcgcg tcgttgaacc acttcacagt tccggttgcc atgttctctt
     3541 taattcctta agtacgagaa ataaacggga cgttgcccgg ccgatgtttc gatctcaagg
     3601 aaagtacggc ggtcgaacga ctgcgtggcg gtcgtacaga gcatgccttt actgcttgga
     3661 acaaaacacg gcgggcacta tacgtcagat ttcgcaaaaa agcgacaact ttccggcggg
     3721 cgggtcgttt gctgttgcgc cggaagccgc gccagacgcg ggattcgggt cgccggccgc
     3781 gcgcaccggg atggcgcgtt aggacgtcaa ttcgcgccgg acgatcgctg cgccttcccc
     3841 cagcgcgcgc agcttctgcc gggcgacggg ccgcgcaagc gggaccatgc cgcagttggt
     3901 gcagggcagc agccggtcgg cgggaacgaa gcgcaaggca ctccggatca cctcggccac
     3961 ctcggcgggc gtctcgatcc ggtcgctggc cacgtcaatg gccccgacca ggacgtcctt
     4021 cccttccagc aatccgatga gctcgatcgg cacatgcgag tgcgcgcact cgagcgacac
     4081 ctggtcgatc gtcgacgcgg cgagccgggg gaaggtctgc tcgtactggc gccactcgcc
     4141 cccgagggag ttcttccagt cgatgttcgc cttgatgccg tagccgtagc aaatgtgcac
     4201 cgccttggtg cattgcaggc cttcggccgc ccgctcgagc gtcgcgacac cccagtcgcg
     4261 aacctcgtcg aagtaaacat tgaaggccgg ctcgtcgaac tgaatgacgt cgacgcctgc
     4321 cgctgcgatg gcgcgcgctt cctggttcag gatctccgca aacgcccagg cgagcttttc
     4381 gcggctgccg tagtgcgcgt cggagagcgt gtcgaccatc gtcatcgggc cgggcagcgt
     4441 gtacttgacc gggcgatcgg tctctgcgcg caggaacgcg gcgtcgtcga cgtagatcgg
     4501 gtggcggcgg gcgaccggcc cgacgaccac gggcaccgac gcgtcgtagc ggttgcggat
     4561 gcgaaccgtc ttgcgatgct cgaaatcgac gccgtcgagg ccctcgatga atgtcgtgac
     4621 gaaatggcgg cgcgtctgct cgccgtcggt cacgacgtcg atgcccgctt gttcctggtc
     4681 gcgcagcacg agccggaccg cgtcgcgctt gccttcggcc agcgcctcgc cgtcgagctt
     4741 ccatgcggcc cacagcgcct cgggctcggc gagccatgac ggcttcggca ggcttccggc
     4801 gatcgtcgtg cgcagcagcg tcatcgtctc ttctccgtca aaaggcaggc gtagcgaccg
     4861 attctatgcc ctcgacgctt gcccgaagta gggtcagaga cgactttcct gttacctttc
     4921 gcaccggcac tgctgtcgaa agtcgtctcc aacctcgctt tgatcgatgt cccgaatcct
     4981 tttcgccgat aacgattttc ccgacgtcga cctcgagcgc gatatcttcg ccggtgcgaa
     5041 cctgtcgctc gagctcgcgc aatgccgcag cgaagcggag gtggtcgctg cggcgcgcgg
     5101 ctgcgttgcc atcctgctgc aatacgcacc gatcaccgcg ggggtggtcg ccgcatcgcc
     5161 gtcgctcggt atcgtgagcc gcatcggcgc gggcttcgac acggtggaca ccgccgcatg
     5221 cgaggcgcac ggcgtatggg tcgccaactc gcccgattat ggcatcggcg aggtcgcgac
     5281 gcatgcactc gcgctggcgc tcgcgtcgat ccgcaatgtc gcgcgctacc acgacgatgt
     5341 gcgcgccggc cgctggcact acctgtcggg cggaacgctg cgccgcccgg cgtcgctcac
     5401 gctcggcatc gtgggcctcg ggcgcatcgg caagcggatg gcgcacgtgg cgcacaacgt
     5461 cgtgaagcgt gtgatcgcct acgacccgct gctcatcgac ggcgactttc ccgcgtacgt
     5521 cgagcgtgcg ccgtcgctgc ccgagcttgc cgcgcagtcc gatatcgtgt cgctgcacac
     5581 gccgctcgac gcgtcgacgc gcgggatgat cgacaagcgt tttttcgccg cggcgaggcg
     5641 ggacatgacc ctcgtcaaca cgtcgcgcgg tggggtcgtc gacatcgccg atttgctcgc
     5701 ggccctcgat gctggaatac tgcggggcgc agcactcgac gtgctgcccg aggagccggt
     5761 cgcgcccgat tcgccgctgc gggccgatcc gcgcatcatc ctgacgccgc atgccgcgtt
     5821 ctattcgatc gaggccgcgc aggagctgcg gcgcaaggcg gcgcagaaca tcgtcacgtg
     5881 gtggcgcacg ggacggcccg actacccggt cgtgcgcggc acgcggaagc cgccgggaac
     5941 atgatgtgac tgacaggacg atgatggcag attggacgaa gcgagccgaa gggccacccc
     6001 gagc
//
LOCUS       DASSVX010000349         8737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1432026, whole genome shotgun sequence.
ACCESSION   DASSVX010000349 DASSVX010000000
VERSION     DASSVX010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432026"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFO53_14920"
     CDS             complement(<1..574)
                     /locus_tag="VFO53_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877619.1"
                     /translation="MISEPSAAIMARVSATPARGYRRIDALTLGTLPFLLILGAWALL
                     PYVADYPSYMLPSIGTVLDYARESVLDGSLPRDVLASLARLVIGFAIGMALAIPLGLA
                     IALNRYVADLFRPVLSFLQAIAGIAWVPLTIIWFGMGNGTVIFVIANTIFFAALYNTV
                     IGVQSIPLALHRAVRSHGGRGVALLANLILP"
     gene            complement(571..1401)
                     /locus_tag="VFO53_14925"
     CDS             complement(571..1401)
                     /locus_tag="VFO53_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8877620.1"
                     /translation="MTPHAALGLPLRGSRPLRRFGATARGFAGVLILLAAWQISVPLV
                     GLGPYFYPAPGDVLAAFVDLVRKGILPVYLADSVMRYLVGLIAGATLGIAFGVLIGMH
                     RRASLALSPLFKFLFAIVEVAWIPIFVVWFGYGIETIVLALIYVVFFPVLYNTLLGVR
                     TAPQILVNAVRSLGASRFQVLRLVILPSALPGIITGLRLGAGFAFRGLVFAEMVAAKT
                     GIGYLIFEGAQSQQTARTIVGMIVMGLLWLAIDQFYLRPIERATIQRWGLTVDAGERT
                     "
     gene            complement(1412..1720)
                     /locus_tag="VFO53_14930"
     CDS             complement(1412..1720)
                     /locus_tag="VFO53_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877621.1"
                     /translation="MLRPPATATAVLLIALGAYWLTGLIGAQHKLGALEPPPSARGDY
                     RITLAFAPERFHQVLLQEKGRLVEVRGNTVFMKDVDPKALRSIARNYWVEAVARWSGS
                     "
     gene            complement(1720..2529)
                     /locus_tag="VFO53_14935"
     CDS             complement(1720..2529)
                     /locus_tag="VFO53_14935"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8877622.1"
                     /translation="MKAGTDKIVIDDLTLDYVNQETGAGHRAVEHLDLRVAADEFLCV
                     VGPSGCGKSTLLSAIAGFLRPTHGSIVMDGRPITGPGAERGVVFQEYALLPWMTVLDN
                     AALGLKLRGMPKKERYGEARRFLALANLHGVEHKYPHELSGGMKQRVAVARTLANTPE
                     VMLMDEPFAAVDAQTRMVLQEELCRISLRSRLTVLFITHSVEEAIFLGDRVAVMSPGP
                     GRIRTIVDVPFARDARTWSTLNADPGFGAMRDRLLDLVRAPHAAQRLEVHA"
     gene            complement(2526..3236)
                     /locus_tag="VFO53_14940"
     CDS             complement(2526..3236)
                     /locus_tag="VFO53_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET8877623.1"
                     /translation="MPTSLDTAPCTVAFDLDGTLAATGPDILRALNLALCEAGLEAAP
                     PERMNELVSLGRGPRVLIEHCFGLNGRSPDSEAVRSIADRYLEIYYENIAVESVLFPG
                     CLPSLEALHGGDYRLAVCTNKPERHAKLLLDALGILPLFSAVFGRDSLPYSKPDPRHL
                     TQTVLAARGDPLRAIMVGDSVTDVATARAADIPVICVAFGYSDVPPETLGGDVLIQGF
                     AELPAAVAALDRARKPST"
     gene            complement(3243..3944)
                     /locus_tag="VFO53_14945"
     CDS             complement(3243..3944)
                     /locus_tag="VFO53_14945"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8877624.1"
                     /translation="MTKPRPLRAATPERAKKAKPRSLMREVAYERFKAELFKRNLAPG
                     QFVSQGELCELLDVPLGPTREALKRLEAENLVRLIPQRGIQITDIGVTLIHEAFQFRT
                     ILEAAAVRHFVTVADDATLSELERITTEVLRRLDGGNNADPRLLDAALKVDWSMHDMI
                     VESVGNRIMMTAHRQNFDKIKMIRLHGRSPRYLPLAMEEHLGVIAAMKQRDPEKAAAA
                     LEHHMRCAERRALGL"
     gene            4066..5106
                     /locus_tag="VFO53_14950"
     CDS             4066..5106
                     /locus_tag="VFO53_14950"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877625.1"
                     /translation="MISIRALRAVLAATAALFLATVASAEQFRVAVTSAAENAPFFSA
                     MEHGTFSKLGLDVKVDVMPSGVEISNALASNTVDVGLFGTFPFLTAVARGVPVVLIGH
                     TWNNALNNPQSEILSVIAREGSGVPAGDLKAMKGKKIGVTRGAGGEPYIVGLLKQVGL
                     TPDDVTLVNTAPSNMATALASKNVDLITSWEPWPSAALTKVPGAYKVIYGGCKSCYDA
                     GTVVTTKSAIKDKSKELGLFIQGYAEAQHWARLHRDEAATIATRWIPGLDEATLKLAF
                     KTLPLDVRLSKNTIEGFSEYSIPLLVRDKRIPKAFDPAPAVDNAFVAAAMKADPSAFD
                     DLKPIPADKRLP"
     gene            5212..6699
                     /locus_tag="VFO53_14955"
     CDS             5212..6699
                     /locus_tag="VFO53_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018209234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET8877626.1"
                     /translation="MSDGRPNLLFLFSDQHARRVAGCYGDPSGATPNLDGLAAEGVAF
                     DNVYCPSPLCVPSRMSMLTARHPHEQECWTNDDYLRSDAATWVHGVGAAGYRPVLAGR
                     LHSMGPDQLHGYAERMVGDHSPNWGGVPRHDLGVLDKANDPWRESLDRSGIGQSAYQV
                     KDDETAKAACTYLRETARARAAGKRDPFCLTVGFLLPHPPYVAWREDYVRFDGRVPAP
                     AHAAPPGDAHPWEAWWRDDRGITDVAVPVTMRARTAYYGLVHRLDALIGDIVACLAEC
                     GLADDTLVVYTTDHGDQLGERGLWWKHTLFEDSVRVPLIMRWPGHIPAGERRAQVANL
                     IDVAATLIDALGGMSLPLGRGRSLLPVVRDPCAAWLDETFCEHCTDVVPAWTGGRATQ
                     QRMVRSGRFKLIYYHGYAPQLFDLDDDPYERGDLAGNPRHARTRDALLARVLDGWDPD
                     AIAARIRARREEKNVIDRWAQQVRPHDAFRWTLLPEHNRLEAEAG"
     gene            6775..7737
                     /locus_tag="VFO53_14960"
     CDS             6775..7737
                     /locus_tag="VFO53_14960"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8877627.1"
                     /translation="MHWPRLGLGCASLGTPSPALRDADAVAVIRAAIERGIRFFDVAP
                     LYGGGVAEERLGRALRDLRRDDYVLCTKTGVTRGYAQPPMPSGATRRRQFDKWDYGDA
                     ATRTSVRSSLARLGVERLDVVHLHDVEDHVDACREAYRALQALREEGIVGTIGIGSNL
                     VAPVQHLLERADFGTFLLAGRYTLLDQSGAALIAHAHAHGIAVVAGGVFNSGVLATWP
                     QPAPTFGYAPADEPVLARTARIAAICAEHDVPIGAAALQFVLANPAITTVLIGPRSVD
                     ELHANLDAVRFPIPDALWSALEAAQLIPGGGPRPMALAALDIAR"
     gene            7750..8601
                     /locus_tag="VFO53_14965"
     CDS             7750..8601
                     /locus_tag="VFO53_14965"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8877628.1"
                     /translation="MRIDAHIHFWRTRCGFDNRPIADHEAYRRDFMPADVEDDLGRCA
                     IDAVILVQTCPQVDETAWLVDLARDSERIAGVTGWVDLDDPRCDFSPLLAERKLVGIR
                     AQLRRIADPAFIERPQVLRNLGTALRANLGVTLLAEPRHYAHVARALDALPPGPITLN
                     HLGLRFAATDATQWRAAMREFARRDDCFVQLSGLPFLYAERWRDAAALAVLDEAFDIL
                     GPSRLVFASDWPMLVRFASYADWVRTVDEFLVRRGVARHDVDAIFAGNVRRANPRLSL
                     PTLAGTS"
BASE COUNT         1451 a         3018 c         2945 g         1323 t
ORIGIN      
        1 cgggcaggat caggttcgca agcagtgcta cgccacgccc gccgtggctg cgcacggcac
       61 gatgcaaggc gagcggaatc gactgcacgc cgattaccgt gttgtagagt gctgcgaaga
      121 agatcgtgtt ggcgatcacg aagatcaccg tgccgttccc catgccgaac cagatgatcg
      181 tgagcggcac ccaggcgatg cccgcgatcg cctgcaggaa cgacagcacc ggccgaaaca
      241 ggtcggcgac atagcgattg agcgcgatcg cgaggccgag tggaatggcg agcgccatgc
      301 cgatcgcaaa gccgatgaca agccgcgcaa ggctcgccag cacgtcgcgc ggcagcgagc
      361 cgtccagcac cgattcgcgc gcgtaatcga gcaccgtgcc gatcgacggc aacatgtacg
      421 acggatagtc ggcgacgtag ggcagcagcg cccacgcgcc caggatcagc aggaaaggca
      481 gcgtgccaag cgtcagcgcg tcgatgcgac ggtagcctct ggccggcgtc gcggacacac
      541 gcgccatgat cgccgccgac ggctcggaaa tcacgtgcgc tctcccgcgt cgacggtcag
      601 cccccagcgc tggatcgtcg cgcgctcgat cgggcgcagg tagaactggt cgatcgcgag
      661 ccacaacaga cccatgacga tcatgccgac gatcgtgcgc gccgtctgct gcgattgcgc
      721 gccctcgaag atgaggtagc cgatgccggt cttcgccgcc accatctcgg cgaacacgag
      781 gccgcgaaac gcgaagcccg caccgaggcg caggccggtg atgatgccgg gcagcgcgct
      841 cggcaggatc acgaggcgca acacctggaa gcggctggcg ccgagcgagc gcaccgcgtt
      901 gacgagaatc tgcggcgcgg tgcgcacgcc gagcagcgtg ttgtagagca ccgggaagaa
      961 aacgacgtag atcagcgcga gcacgatcgt ctcgatgccg tagccgaacc agacgacgaa
     1021 gatcggaatc cacgcgacct cgacgatcgc gaacaggaac ttgaagagcg gcgacagcgc
     1081 gagcgacgcc ctgcgatgca tgccgatcag cacgccgaac gcgatgccga gcgtcgcacc
     1141 cgcgatgagt cccacgaggt agcgcatcac gctgtcggcg agatagaccg gcaggatgcc
     1201 cttgcgcacg agatcgacga acgccgccag cacgtcgccc ggcgccgggt agaagtacgg
     1261 gccgaggcca acgagcggca cggagatctg ccacgccgca agcaaaatca gcacgccggc
     1321 aaatccgcgc gccgtcgcgc caaagcgacg cagtggccgc gatcctcgca gcggcagccc
     1381 gagcgcggcg tgcggcgtca tgcgtcgccg ctcacgatcc gctccaccgc gccaccgcct
     1441 cgacccagta gttgcgcgcg atcgagcgca acgccttcgg atcgacgtcc ttcatgaaca
     1501 ccgtattgcc gcgcacctcg acgaggcgtc ccttctcctg cagcagcacc tggtggaagc
     1561 gctcgggcgc gaaggccagc gtgatccggt aatcgccgcg cgcggacggc ggcggctcca
     1621 gcgcgccgag cttgtgttgc gcgccgatca ggcccgtgag ccagtaagcg ccgagcgcga
     1681 tcagcaggac ggccgtcgcg gtcgcgggcg gccgcagcat caggcgtgca cctccaggcg
     1741 ttgcgccgca tgcggtgcac gcacgaggtc gagcagccgg tcgcgcatcg cgccgaagcc
     1801 cggatcggcg ttgagcgtgc tccatgtgcg cgcatcgcgt gcgaacggca cgtcgacgat
     1861 cgtgcggatg cgacccggtc cgggcgacat cacggcgact cggtcgccga ggaagatcgc
     1921 ctcctcgacg ctgtgcgtga tgaacagcac cgtgagccgc gagcgcagcg aaatgcggca
     1981 gagctcctcc tgcagcacca tgcgcgtctg cgcatcgacg gcggcgaacg gctcgtccat
     2041 cagcatcacc tcgggggtgt tcgcgagcgt gcgcgccacg gcgacgcgct gcttcatgcc
     2101 gccggagagc tcgtgcgggt acttgtgctc gacgccgtgc aggttcgcga gtgccaaaaa
     2161 gcgtcgcgct tcgccgtagc gctccttttt cggcatgccg cgcaacttca aacccagcgc
     2221 cgcattgtcg agcaccgtca tccacgggag cagcgcgtat tcctggaaca cgacgccgcg
     2281 ctcggcgccg gggccggtga tcggccggcc gtccatcacg atgctgccgt gcgtgggacg
     2341 caggaagccg gcgattgccg aaagcagcgt cgacttgccg cagccgctgg ggccgacgac
     2401 gcaaaggaat tcgtcggccg cgacgcgaag gtcgagatgc tcgacggcgc ggtggcccgc
     2461 accggtctcc tgattgacgt agtcgagcgt caggtcgtcg atgacgatct tgtccgtgcc
     2521 ggctttcatg tcgatggctt ccgcgcgcga tcgagcgcgg cgaccgccgc gggaagctcc
     2581 gcgaagcctt gaatcagcac gtcgccgccg agcgtctccg gcgggacgtc ggagtagccg
     2641 aacgcaacgc agatgacggg aatgtctgcg gcgcgggctg tcgcaacgtc ggtcacgctg
     2701 tcgccgacca tgatcgcgcg cagagggtct ccgcgtgcgg cgaggacggt ctgcgtgagg
     2761 tggcgcgggt cgggcttcga atacggaagc gagtcgcgcc cgaacacggc ggagaacagc
     2821 ggaaggatgc ccagcgcgtc gagcaacagc ttcgcgtggc gctcgggctt gttcgtacac
     2881 accgcgaggc gatagtcgcc gccatgcaac gcttcgagcg acggcaaaca gccggggaag
     2941 agcaccgact ccacggcaat gttctcgtag tagatctcga ggtagcggtc ggcaatgctg
     3001 cgcaccgctt ccgaatcggg cgagcggccg ttcaacccga agcagtgctc gatcagcaca
     3061 cgcgggcctc ggccgaggct cacgagctcg ttcatccgct ccgggggcgc agcttcgaga
     3121 ccggcctcgc agagcgccag gttgagcgcg cgcaggatat cgggtccggt ggccgcaagc
     3181 gttccgtcca ggtcgaacgc gaccgtgcac ggcgcggtgt cgagcgacgt cggcacggcc
     3241 cgctagagtc cgagcgcgcg ccgttcggcg cagcgcatgt gatgctcgag ggcagcggcg
     3301 gctttttccg ggtcccgctg cttcatcgcc gcgatcacgc cgagatgctc ctccatcgcg
     3361 agcggcaggt agcgcggcga acgcccgtgc aggcgaatca tcttgatctt gtcgaagttc
     3421 tgccggtgcg cagtcatcat gatccggttg ccgaccgact ccacgatcat gtcgtgcatg
     3481 ctccagtcga ccttcaacgc cgcgtcgagc agacgtggat cggcattgtt gccgccatcg
     3541 aggcgccgca gcacctcggt cgtgatccgt tcgagctcgg acagcgtcgc gtcgtcggcg
     3601 accgtgacga aatgacgcac ggccgccgcc tcgaggatcg tgcggaactg gaacgcctcg
     3661 tggatcagcg tgacgccgat gtcggtgatc tggatgccgc gctgcggaat gagccgcacg
     3721 aggttctcgg cctcgagccg cttcagcgcc tcgcgtgtgg gaccgagcgg aacgtcgagc
     3781 aattcgcaaa gctcgccctg cgagacgaac tgtcccggcg ccaggttgcg cttgaagagc
     3841 tcggccttga agcgctcgta cgccacctcg cgcatcagcg acctcggctt cgcctttttc
     3901 gcgcgctcgg gcgtcgcggc ccgcagtgga cgcggtttcg tcatcgaatc atccgtcgcg
     3961 gcttgtcagc cgatcatagc attgttagga taccattgag atatcactca catcccggca
     4021 ggcacagcct gccccgccac gattcactcc aaggaggatg actgcatgat ctcgatccgt
     4081 gcactacgcg ccgtgctggc cgccacggcc gccctgttcc tggcgacggt tgcgtccgcc
     4141 gagcagtttc gggtggctgt cacgtcggcc gccgagaacg cgccgttctt ctcggcgatg
     4201 gaacacggca cgttctcgaa actcggcctc gacgtcaagg tcgacgtgat gccgagcggc
     4261 gtcgaaatct cgaacgcgct cgcgagcaac accgtcgacg tcggcctctt cggcaccttc
     4321 ccgtttctca cggcggtcgc acgcggcgtg ccggtcgtgc tgatcggtca cacatggaac
     4381 aacgcgctca acaatccgca aagcgagatc ctctcggtga tcgcgcgcga aggctcgggc
     4441 gtgccggccg gcgacctgaa ggcgatgaag gggaagaaga tcggcgtcac ccgcggcgcc
     4501 ggcggtgagc cgtacatcgt cggcctgctg aagcaggtcg gcctcacgcc cgacgacgtc
     4561 acgctcgtca acaccgcgcc atcgaacatg gcgaccgcac tcgcaagcaa gaacgtcgac
     4621 ctgatcacgt cgtgggagcc ctggccgagc gccgcgctga cgaaggtgcc cggcgcgtac
     4681 aaggtgatct acggcggctg caagagctgc tacgacgccg gcacggtcgt cacgacgaag
     4741 tcggcgatca aggacaagtc taaggagctc ggcctgttca tccagggcta cgcggaggcg
     4801 cagcactggg cgcggctgca ccgcgacgag gcggcaacga tcgcgacgcg atggatcccc
     4861 gggttggacg aggcaacgct caagctcgcg ttcaagacgc tgccgctcga cgtgcggctg
     4921 tcgaagaaca cgatcgaagg cttcagcgaa tacagcatcc cgctgctcgt ccgcgacaag
     4981 cgcattccca aggcattcga tcccgcgccg gccgtcgaca atgcgttcgt cgcggcggcg
     5041 atgaaggccg acccgtcggc gttcgacgat ctgaagccga tcccggccga caagcgcctg
     5101 ccgtgacccg catgcgggcg tcgcgtgccc tccgggaacc catgagtgac ggccggccca
     5161 accccacgag tcacggccgg cccaaccggt tgcgacggcg cggcgaacgc aatgagtgac
     5221 ggccggccca acctgctgtt cctgttttcg gaccagcatg cgcggcgcgt tgccggctgc
     5281 tatggcgatc cgagcggtgc gaccccgaac ctcgacggcc tcgccgccga gggcgtcgcg
     5341 ttcgacaacg tctactgccc gtcgccgctt tgcgtgccga gccggatgtc gatgctcacg
     5401 gcgcgccatc cgcacgagca ggagtgctgg accaacgacg attacctgcg ttccgacgcc
     5461 gccacctggg tgcacggcgt cggcgccgcc ggctatcgtc cggtgctcgc gggccggctc
     5521 cattcgatgg ggcccgacca gctgcacggt tatgccgagc gcatggtcgg cgatcacagc
     5581 ccgaactggg gcggcgtgcc gcggcacgat ctcggcgtgc tcgacaaggc caacgatccg
     5641 tggcgcgaga gtctcgaccg ctcgggcatc ggacagtccg cgtaccaggt caaagacgac
     5701 gagacggcaa aggctgcttg cacctacctg cgcgaaacgg cgcgggcgcg cgcagcgggc
     5761 aagcgcgatc cattctgcct gaccgtcggc ttcctgctgc cgcatccgcc gtacgtcgcg
     5821 tggcgcgagg actacgtgcg cttcgacggc cgcgtgcccg cgcccgcgca tgcggcgccg
     5881 cccggcgatg cgcatccgtg ggaagcgtgg tggcgcgacg accgcggcat caccgacgtc
     5941 gcggtgccgg tgacgatgcg ggcacgcacc gcgtactacg ggctcgtgca ccgcctcgat
     6001 gcgctgatcg gcgacatcgt cgcgtgtctt gccgaatgcg gcctcgccga cgacacgctc
     6061 gtcgtctata cgacggacca cggcgaccag ctcggcgagc gcgggctgtg gtggaagcac
     6121 acgctgttcg aggattcggt gcgcgtgccg ctgatcatgc gctggcccgg gcatattccg
     6181 gccggcgagc ggcgcgcgca ggttgcaaat ctgatcgatg tggccgctac gctcatcgat
     6241 gcgctcggcg gcatgtcgct gccgctcggg cgcggccgca gcctgctgcc ggtcgtgcgc
     6301 gacccgtgcg cggcctggct cgacgagacg ttctgcgagc attgcaccga cgtcgttccg
     6361 gcgtggaccg gcggccgcgc gacgcagcag cggatggtgc gaagcggccg cttcaagctc
     6421 atctactacc atggttacgc gccgcagctc ttcgatctcg acgacgatcc gtacgagcgc
     6481 ggcgatctcg ccggcaaccc gcgtcatgcc cgaacgcgcg acgcgttgct ggcgcgcgtc
     6541 ctcgacggct gggatcccga cgcgatcgcc gcgcgcattc gtgcgcggcg cgaggaaaaa
     6601 aacgtcatcg atcgctgggc gcagcaggtg cggccgcacg acgcgtttcg ctggacgctg
     6661 ctgcccgagc acaaccggct cgaagcggaa gcgggctagg caaacatgcc ttcacgcccg
     6721 ctgcccccac gggggctggc cagtcggcac ggcgcgactg acgtcgcgcc attgatgcac
     6781 tggccccgcc tcggcctcgg ttgcgcgagc ctcggcacgc cctcccctgc gcttcgcgac
     6841 gccgacgcgg tcgcggtgat tcgcgcggcg atcgagcgcg gcattcgctt cttcgacgtg
     6901 gcaccgctct acggtggcgg cgtcgccgag gagcgcctcg gtcgtgcact gcgcgacctg
     6961 cggcgcgacg actacgtgct gtgcacgaag accggcgtca cgcgcgggta cgcccagccg
     7021 ccgatgccgt ccggtgcgac acgacggcgt cagttcgata aatgggacta tggcgacgcg
     7081 gcaacccgca cgtcggtgcg ctcgagtctc gcgcgccttg gcgtcgagcg actcgatgtc
     7141 gtgcatctgc acgacgtcga ggaccatgtc gatgcctgca gggaagcgta tcgcgcgctg
     7201 caggcgttgc gcgaagaagg aatcgtcgga accatcggca tcggctcgaa cctcgtcgcc
     7261 ccggtgcagc acctgctcga gcgcgccgac ttcggcacgt tcctgctcgc cggccgctac
     7321 acgctgctcg atcagtcggg tgccgcgttg atcgcgcatg cgcacgcgca cggcatcgcg
     7381 gtggttgccg gcggcgtctt caactcgggc gtgctcgcga cgtggccgca gcccgcaccg
     7441 acgttcggtt acgctcctgc cgacgagccc gtgcttgcgc gcacggcacg gatcgccgcc
     7501 atctgcgccg agcacgacgt gccgatcggc gccgcggcgc tgcagttcgt gctcgccaac
     7561 ccggcgatca cgacggtgct gatcgggccg cgcagcgtcg atgaactgca cgcgaatctc
     7621 gacgccgtgc gcttcccgat ccccgacgcg ctgtggtccg cgctcgaggc cgcgcagctc
     7681 atcccgggcg gcggtccgcg acccatggcg ctcgccgcgc tcgatatcgc ccgctgaggc
     7741 ccgacgagca tgcgaatcga cgcgcacatc cacttctggc gcacgcgctg cggcttcgac
     7801 aacaggccga tcgccgacca cgaggcatac cggcgcgatt tcatgccggc cgacgtcgag
     7861 gatgaccttg gacgctgcgc gatcgacgcg gtcatcctcg tgcagacctg cccgcaggtc
     7921 gacgaaaccg catggctcgt cgacctcgcg cgagacagcg agcgcattgc aggcgtcacc
     7981 ggctgggtgg atctcgacga tccgcggtgc gacttctcgc cgctgctcgc ggagcgcaag
     8041 ctcgtcggaa tacgcgcaca attgcgccgc atcgccgatc ctgcattcat cgagcggccg
     8101 caggtgctgc gcaatctcgg cacggcgctt cgcgcgaacc tcggcgtgac cctgcttgcc
     8161 gagccgcgcc attacgcgca cgtcgcgcgg gcgctcgacg cgctgccgcc gggaccgatc
     8221 acgctcaatc atctcggcct gcgcttcgcc gctaccgacg cgacgcagtg gcgcgcggca
     8281 atgcgcgaat tcgcgcgtcg cgacgattgc ttcgtccagc tctcgggcct gccgttcttg
     8341 tacgccgagc gctggcgcga cgcagcggcg ctggcggtgc tcgacgaagc gttcgatatc
     8401 ctcggcccgt cgcggctcgt gttcgcgagc gactggccga tgctggtgcg cttcgcgtcg
     8461 tatgccgact gggtgcgtac cgtcgacgaa ttcctggtgc gccgaggcgt tgcgcgccac
     8521 gacgtcgacg cgatcttcgc cggcaacgtg cgccgcgcca acccgcgact ttcacttccc
     8581 acgcttgcag gaacttcatg aaacgatcac agatcaacgc cgcgatcgag cttgcccacc
     8641 ggacgctgcg cgagcaaggc atcgcccttc ccgaatatgc ctactggtcg cccgaccact
     8701 ggcgccgcag ggccggtccc gagcatcgcc gcatttt
//
LOCUS       DASSVX010000350         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_165543, whole genome shotgun sequence.
ACCESSION   DASSVX010000350 DASSVX010000000
VERSION     DASSVX010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165543"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VFO53_14970"
     CDS             <1..933
                     /locus_tag="VFO53_14970"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET8877629.1"
                     /translation="GVGNGTDALELALRATGVDRGDRVAVVANAGYYAGTALAAIGAE
                     PVFVDVDETLTMSPASLEASIRGVDAVVVTHLYGRLAAIEPIVALANEYGIAVIEDCA
                     QAHGAARNAQRAGSFATAGCFSFYPTKNLGALGDAGAVVTSDDEVSERLRALRQYGWS
                     TKYRVEHPGGRNTRLDELQAAFLRVKLPRLAAWNAARVSIARHYHRGFAGLGITLPDF
                     SDDEYVAHLFVIRVQHREALRARLREAGIGTDVHYPIPDHRQPVRMLDHLELPMTEAA
                     CREVVSLPCYPGLSLTHVDRVVDLVRSFCASAQA"
     gene            complement(977..>2656)
                     /locus_tag="VFO53_14975"
     CDS             complement(977..>2656)
                     /locus_tag="VFO53_14975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8877630.1"
                     /translation="VQRDCPVNVRLVGKLSEEDKLQYLQSADIAVNPMFAGSGTNIKM
                     FDFMAAGLPVVATSMGARGICGETRYGVIVCDADRMTGAIARLVADGATCRRLGEANR
                     EWVMRDFSWEKLSPALGVKLRESLAARVSGRAAGRAASAGPVDIGRIDEPQANRATLH
                     DAARVAILSTFGIRCGIAEYTSYLAESLLDAGTELTIVANRMDGHDAAGVCVPERLRG
                     ASVERIWQYDNVRWTEGRVETRDVIELLRTRRVNHLNVQYHRGFFAESELIDLLQRVL
                     ADGIAVSVSLHNSTDSTPHLLAALAAMPITVVVHRRAERDRLRALGVRGATYIPQGIR
                     TPPVIGEAMPRALAVRGGRPPVIGTFGFLRPHKGLLELIDAVSILRAVFPGLRLLAQT
                     ALYPSADSAAYLQKVTQRIDALALADAITLDHGFRDIDEVITTLTAVDVVVLPYARSD
                     EGSSAAAAAALAARRALITTSAPIFDELRGVAYRAEDNSPPVLAAAIGTVLSNDALCG
                     HLGRRSGRYADSHVWGAVARQFLDLVEMQRHERTDVVDGSGRAHMDTAARV"
BASE COUNT          456 a          896 c          868 g          436 t
ORIGIN      
        1 ggcgtcggca acggcaccga cgcgctcgag cttgcacttc gcgcaacggg cgtcgatcgc
       61 ggcgatcgcg ttgccgttgt cgccaacgcc ggctattacg cgggcaccgc gctcgccgcc
      121 atcggcgccg agccggtgtt cgtcgacgtg gacgaaacgc tgacgatgtc gccggcgtcg
      181 ctcgaggcgt cgatccgggg cgtcgacgcc gtcgtcgtca cccacctgta cgggcggctc
      241 gcagcgatcg agccgatcgt tgcactcgcg aatgagtatg ggatcgcggt gatcgaggat
      301 tgcgcacagg cgcacggtgc ggcgcggaac gcgcagcgcg ccggctcgtt cgcaacggcc
      361 ggctgcttca gcttctatcc gacgaagaat ctgggcgcgc tcggcgacgc cggtgcggtc
      421 gtcacgtctg acgacgaggt ttcggagcgc ctgcgcgcgc tgcgccagta cgggtggtcg
      481 accaagtatc gcgtcgagca tccgggtggc cggaacacgc ggctcgacga gttgcaggcc
      541 gcattcctcc gcgtcaagct gccgcgcctc gccgcctgga atgcggcacg ggtgtcgatt
      601 gcccgccact accaccgtgg gtttgccgga ctgggaatca cgctgcccga tttcagcgac
      661 gatgagtacg ttgcgcacct cttcgtcatc cgggtgcagc accgggaagc gcttcgcgcg
      721 cgccttcggg aggcaggaat cggaaccgac gtgcattacc ccattcccga ccatcggcaa
      781 ccagtgcgca tgctggacca cctcgagttg ccgatgaccg aggcggcgtg ccgcgaggtc
      841 gtgtcgctcc cgtgctaccc cggactgtcg ctgacgcacg tcgatcgcgt cgtcgacctc
      901 gtacggtcgt tttgcgcaag tgcacaagcg tagcgcgcct cccggttcac gcgcgagcaa
      961 caccaggcgc caagcgtcaa acgcgagccg ccgtgtccat atgcgcgcga cccgaaccat
     1021 ccacgacatc ggtgcgctcg tgacgttgca tttcgaccag gtcgaggaac tgccgggcaa
     1081 cggcgcccca gacgtgcgaa tcggcgtatc ggccggagcg tcgcccgaga tggccgcaaa
     1141 gtgcatcgtt tgacaacacc gtgccgatcg ccgcggccag gaccggcggc gaattgtctt
     1201 cggccctgta tgcaacacct cgcaactcat cgaagatcgg cgcgctcgtc gtgatgagcg
     1261 cacgccgcgc agccagcgcg gcggccgccg cggcgctcga accctcgtcg gagcgcgcat
     1321 aaggcagcac gaccacatcg acggccgtaa gtgtcgtgat cacttcatcg atgtcgcgaa
     1381 atccgtgatc cagcgtgatc gcatcggcga gcgccagtgc gtcgattcgc tgcgttacct
     1441 tctgcagata cgccgcggaa tcggccgacg gatagagcgc ggtctgcgca agcagccgga
     1501 gcccgggaaa tactgcgcgc aggatcgaca ctgcatcgat caactcgagc agccccttgt
     1561 gcgggcgcag aaacccgaat gtgccgatca cgggcggtcg gccgccgcgg actgcgagcg
     1621 ctcgcggcat cgcttcgccg atgaccggcg gcgttcgaat gccctgggga atgtacgttg
     1681 cgccgcggac gccgagggcg cgcaaccggt cgcgctcggc gcggcgatga acgacgaccg
     1741 tgatcggcat cgcggcaagc gcggcaagca gatgcggcgt cgaatcggtc gaattgtgta
     1801 atgaaacgct gaccgcgatg ccgtctgcga gaacgcgctg cagcaggtcg atcaattcgc
     1861 tttccgcgaa aaagccgcga tgatattgca cgttgaggtg attcacacgt cgcgtccgga
     1921 gcagctcgat gacatcgcgc gtctcgaccc gaccttccgt ccagcgcaca ttgtcgtatt
     1981 gccagatccg ctcgacactt gcgccacgca atcgctcggg tacgcacacg cctgcagcgt
     2041 cgtgcccgtc catgcggttt gcgacgatcg tcaactcggt tccggcatcg agcagcgact
     2101 cggcgagata actcgtgtat tcggcgatgc cgcagcggat gccgaacgtc gacaggatcg
     2161 cgacccgcgc cgcgtcgtgc agggttgcgc gattggcctg cggctcgtcg atccggccga
     2221 tatcgacggg tcccgcgctg gcggcacgcc ccgcggcacg gcccgacacg cgggctgcga
     2281 gcgattcgcg cagttttacg ccgagcgcgg gcgacagctt ctcccatgaa aagtcgcgca
     2341 tgacccattc gcggttggcc tccccgagcc gccggcatgt cgcgccgtcg gcgacgaggc
     2401 gggcgattgc gccggtcatg cgatcggcat cgcaaacgat cacgccgtac cgcgtctcgc
     2461 cgcaaatgcc ccgcgcaccc atggatgtcg caacgacggg caatccggcc gccatgaaat
     2521 cgaacatctt gatgttggtg cccgagcccg caaacatcgg attcaccgcg atatcggcgg
     2581 actgcaggta ctgcagcttg tcctcttcgg acagcttccc gacgagacgc acgttcacgg
     2641 gacaatcgcg ctgcac
//
LOCUS       DASSVX010000351         4629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_982148, whole genome shotgun sequence.
ACCESSION   DASSVX010000351 DASSVX010000000
VERSION     DASSVX010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982148"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..2338)
                     /locus_tag="VFO53_14980"
     CDS             complement(116..2338)
                     /locus_tag="VFO53_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET8877631.1"
                     /translation="MTAISASKLIGQPLDRTDGPLKTTGGARYAAEFRLPDICHGVMV
                     LSTIPRGRIVDLDATRAEAAPGVLLVLSHRNAPSLPDHGRAAFKPPAGRALSLFQDDE
                     VHYNRQPIAVVVADTFEHAVAAAALVRARYASEPPRLDFERERAAAHKPKDMNGKPPD
                     ASWGDMAAGERAAEVRFDATYTTPMQSHNPLEPHATIAQWEGDRLTLYDATQYVSGVR
                     EAVAKTLGIADDNVRVVSPFVGGGFGCKGSAWSHVVLAAMCARNVARPVKLVVDRQQM
                     FGPVGGRPLTVQRVALGARRDGTLTYVRHDCVSHTSDFEDWTEPAAVVSRMMYACANG
                     STTHRLVKLNTGTPTFQRAPGEATGNFALEVAMDELAAALALDPLALRLRNYAEQEAD
                     TGKPFSSKHLRECYRDAADRFGWSQRSAAPRSMRNAGQFVGFGMATASYPAHRMAASA
                     RAALSPDGSFVVQSGSQDLGTGTYTVMTQVAAETLGVPASRVRFELGDTRLPHAPVSG
                     GSMSAASVGPAVQAACRQLRDLLIGQAVADAASPLHAAAPSNVTVDGGWLAVRDEPSR
                     RETIAALAGRAGHPLEATAEAKPGNESERYGLHSFGAVFAEVHVDPDLGVIRVPRIVA
                     AYDIGRPLNAKTARSQLQGGIVWGVSFALLEDARMDPRNGRIVNANLAEYHVPVNADI
                     QTIDVTFVDHPDFAFNPLGIRGIGEIGITGVAAALSNAIQHATGRRLRDLPMTLDKLL
                     "
     gene            complement(2335..3318)
                     /locus_tag="VFO53_14985"
     CDS             complement(2335..3318)
                     /locus_tag="VFO53_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET8877632.1"
                     /translation="MRAFEYVRAARIDEAIAALADPDTRCLAGGTNLLDLMKGYVEQP
                     ARLLDISRLPLAGIQALPDGGLRIGALVPNSDLANDRNVRERYPLLSQALVSGASPQL
                     RNMATVGGNLLQRTRCHYFTDVAFDACNKRTPGSGCAAREGPNRIHAILGASEQCIAV
                     HPSDMCVALAALDGVVEAQSRSGVRRIPMSEFHRLPGTTPERDTELRHDELVIAVTLP
                     PSPFAAHSHYLKVRDRASYEFALVSVAAALDLAGDRVRDARIVLGGVAHRPWRARGAE
                     QALVGHTLDDAAIAASAAAASDGARPYRDNAFKVTLAQRAVARALREAKAT"
     gene            complement(3321..3896)
                     /locus_tag="VFO53_14990"
     CDS             complement(3321..3896)
                     /locus_tag="VFO53_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET8877633.1"
                     /translation="MAADNNLAPSPTTRAGGPGAPRRVQLNVNDHDYDLMLEPRVTLL
                     DALREYIGLAGTKKGCDRGQCGACTVLVDDRRINSCLALAAAHEGHRIRTVEGLGGAT
                     ELHPMQAAFIEHDGFQCGYCTPGQICSAVGMLSEARADTVSAVTADVRRRGPVELTDA
                     EIRERMSGNLCRCGAYVGIVAAVRDASSRMK"
     gene            complement(4041..4445)
                     /locus_tag="VFO53_14995"
     CDS             complement(4041..4445)
                     /locus_tag="VFO53_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877634.1"
                     /translation="MKRLLLRLTTATAMIASGTALACGACVEDKVAATYDHAVISQAK
                     ARHQQVLFVALDGPDAERAAPRIAAVAAKVHGVEAKTLRMSASPAAFSIAAARSEPPE
                     RAVAAFRDALRDPAMQLTLVRVMRDGMLIEPK"
     gene            complement(4442..>4629)
                     /locus_tag="VFO53_15000"
     CDS             complement(4442..>4629)
                     /locus_tag="VFO53_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877635.1"
                     /translation="EFIAYFKWADENLRPKGEQQPQPAAPGTTLPPGRTLSGSPGEVR
                     PSGAPQAQSTEKGKGKR"
BASE COUNT          696 a         1613 c         1602 g          718 t
ORIGIN      
        1 cccccgacac gggtgcgtgc ggcaatcgcg tatcgccgag ctcgaagcgc acgcgcgttc
       61 gtcaatacgt gcgcctgccc ggcatgcgtg gcgtcgtgcg cgcggacgag cggtgctaca
      121 gcagcttgtc gagcgtcatc ggcagatcgc gcaggcgccg gccggtcgca tgctggatgg
      181 cgttcgacag cgccgccgca acgccggtga tcccgatctc tccgatgccg cggatgccga
      241 gcgggttgaa cgcgaaatcg gggtggtcga cgaacgtcac gtcgatcgtc tggatgtcgg
      301 cattgaccgg cacgtggtat tcggcaaggt tcgcgttcac gatgcggccg ttgcgcgggt
      361 ccatccgggc atcctcgagc agcgcgaagc tcacgcccca cacgatgccg ccctgcagtt
      421 gactgcgcgc cgtcttcgca ttgagcggac gtccgatgtc gtacgcggcg acaatgcgtg
      481 gaacgcggat gacgccgagg tcggggtcga cgtgcacctc ggcgaacacc gcaccgaacg
      541 aatgcaggcc atagcgctcg ctttcgttac ccggtttcgc ctcggcggtt gcctcgagcg
      601 gatgtcccgc gcgcccggcc agcgcggcga tcgtctcgcg ccgcgacggt tcgtcacgaa
      661 ccgcgagcca tccgccatcg accgtcacat tcgaaggcgc ggcggcgtgc aacggcgacg
      721 ctgcatcggc aacggcctgt ccaatcagca ggtcgcgaag ctgccggcac gcggcctgca
      781 ccgcggggcc gacgctggcc gcgctcatcg atccccccga cacgggtgcg tgcggcaatc
      841 gcgtatcgcc gagctcgaag cgcacgcgcg acgccggaac gccgagcgtc tcggcggcca
      901 cctgcgtcat taccgtgtag gttccggtgc cgaggtcctg cgagcccgat tgaacgacga
      961 acgaaccgtc gggcgacagc gcggcacgcg ccgaagcggc catccggtgc gcggggtacg
     1021 aggccgtcgc catgccgaag cccacgaatt gtccggcgtt gcgcatcgat cgcggcgccg
     1081 cgcttcgctg cgaccagccg aaccggtcgg ccgcgtcgcg atagcattcg cgcaggtgct
     1141 tgctcgagaa cggcttgccg gtgtccgcct cctgctcggc gtagttgcga aggcgcagcg
     1201 ccagcggatc gagcgccagt gccgcggcca gctcgtccat cgcgacttcg agcgcgaaat
     1261 tgccggtggc ttcgccgggc gcgcgctgga acgtgggcgt ccccgtgttg agcttcacca
     1321 gccggtgcgt cgtcgatccg ttcgcacacg catacatcat tcgcgacacg acagcggccg
     1381 gctccgtcca gtcctcgaag tcggacgtgt gcgaaacgca gtcgtggcga acgtaagtca
     1441 acgttccatc gcgccgcgca ccgagtgcga cgcgctgcac ggtcagcggc cgcccgccga
     1501 ccggcccgaa catctgctgg cggtcgacca cgagcttgac cggccgtgcc acgttgcgcg
     1561 cgcacatcgc cgcgagcacc acgtgcgacc acgccgaccc cttgcagccg aagccgccgc
     1621 cgacgaacgg tgacaccacg cgcacgttgt cgtcggcgat gccgagcgtc ttcgccaccg
     1681 cctcgcgcac gcccgacacg tattgcgtcg cgtcgtagag cgtcaaccga tcgccctccc
     1741 actgcgcgat cgtcgcatgg ggctcgagcg gattgtggct ttgcatcggc gtcgtgtacg
     1801 tcgcgtcgaa gcgcacctcg gccgcacgct cacccgccgc catgtcgccc cagctcgcgt
     1861 cgggcggctt cccgttcatg tccttcggct tgtgcgcggc ggcccgctcc cgttcgaagt
     1921 cgagccgcgg cggctcgctc gcgtagcgcg cgcgaaccag cgcagcggcc gcaaccgcgt
     1981 gctcgaacgt atccgccacg acgaccgcga tcggttgccg gttgtaatgc acttcgtcgt
     2041 cctggaagag cgacaacgcg cgtccggcag gcggcttgaa ggcggcacgg ccgtgatcgg
     2101 gaaggctcgg ggcgttccgg tgcgacagca cgagcaggac acccggggca gcctcggcgc
     2161 gcgtcgcgtc gagatcgacg atgcgtccgc ggggaatcgt cgacagcacc atgacgccgt
     2221 gacaaatgtc gggtaagcga aactccgcag cgtagcgcgc accgccggtg gttttcagcg
     2281 gcccgtccgt gcgatcgagc ggctggccga tgagcttcga cgcggaaatc gcggtcatgt
     2341 tgccttggcc tcgcgcagcg cacgcgcgac cgcgcgttgc gccagtgtca ccttgaacgc
     2401 attgtcgcga taaggccgcg ccccgtcgct cgctgccgcg gcggaggcgg caatcgccgc
     2461 atcgtcgagc gtgtgcccga ccagcgcctg ctcggcgccg cgcgcccgcc acggccggtg
     2521 cgcgacgccg ccgagaacga ttcgcgcgtc acgaacgcga tcgccggcga ggtcgagtgc
     2581 cgcggcaacg gacacgagcg cgaattcgta gctcgcgcga tcgcgcacct tgagatagtg
     2641 cgagtgcgcg gcgaacggcg agggcgggag cgtcaccgcg atgacgagct cgtcgtggcg
     2701 cagctcggtg tcgcgctcgg gcgtggttcc gggcaggcga tgaaactcgc tcatcggaat
     2761 gcggcgcacg cccgatcggc tttgcgcttc gaccacgccg tcgagtgcgg caagggccac
     2821 gcacatgtcg gaggggtgca cggcgatgca ttgctcgctt gcgccgagga tcgcgtggat
     2881 gcggttcggg ccctcgcgcg ccgcgcagcc cgagccgggc gtgcgcttgt tgcaggcgtc
     2941 gaacgcgacg tcggtgaagt aatgacagcg cgtgcgctgc agcagattgc cgccgaccgt
     3001 ggccatgttg cgcagctgtg gcgacgcgcc ggaaacgagc gcctgcgaaa gcagcggata
     3061 gcgctcgcgc acgttgcgat cgttggcgag gtcgctgttg ggaacgagcg caccgatccg
     3121 caacccgccg tcgggcagcg cctggatgcc ggcgagcggc aggcggctga tgtcgagaag
     3181 acgcgccggc tgctcgacgt accccttcat caggtcgagc agattggtgc cgccggcaag
     3241 gcagcgcgtg tcgggatcgg cgagcgccgc aatggcctcg tcgatgcggg cggcgcgcac
     3301 atactcgaac gcgcgcatcg ctatttcatc cgcgacgacg cgtcgcgcac ggcggcgacg
     3361 atgccgacgt aagcaccgca gcggcacagg ttgccgctca tccgttcgcg gatttcagca
     3421 tcggtgagct cgacgggacc gcgccggcgc acgtcggcgg tgaccgcgct cacggtgtct
     3481 gcgcgcgctt cgctcagcat gccgaccgcc gagcagatct gccccggtgt gcaatagccg
     3541 cactggaagc cgtcgtgctc gatgaacgcc gcctgcatcg ggtgcagctc ggtggcgccg
     3601 cccagtcctt cgacggtacg aatgcgatgg ccctcgtgcg cggccgcgag tgcgaggcac
     3661 gaattgatgc gccgatcgtc gacgaggacc gtgcacgcgc cgcactgccc gcgatcgcaa
     3721 cctttcttgg tgccggcaag gccgatgtat tcgcgcaacg cgtcgagcag cgtcacgcgc
     3781 ggctcgagca tcaggtcgta gtcgtgatcg ttgacattga gctgcacgcg ccttggcgcg
     3841 ccggggccgc ccgcccgcgt cgtgggcgat ggcgcgaggt tgttatcggc ggcgataaga
     3901 ggaggcacgt gcgcgagcgc tgtcgtcaat gcgcccgttc cgcagaggaa gcgccggcgc
     3961 gcggcgccgt tcgctgtcag gcgacgcctg tccatcccat caagaccggc accccgcgct
     4021 cgggttccgg ccgcacgccg ttatttcggc tcgatcagca tgccatcgcg catgacgcgc
     4081 acgagcgtca actgcatcgc gggatcgcgc agcgcgtcgc gaaaggcggc gacggcccgc
     4141 tcgggcggct ccgagcgtgc agcggcgatc gagaacgccg cgggcgacgc agacatgcgc
     4201 agcgtcttcg cctcgacccc gtgcaccttc gctgccacgg ccgcgatgcg cggcgccgcg
     4261 cgctcggcgt ccggcccgtc gagcgcgacg aacagcacct gctggtgcct ggccttcgcc
     4321 tgcgaaatca ccgcatggtc atacgtcgcc gcgaccttgt cctcgacgca tgcgccgcac
     4381 gcgagtgctg taccggaggc gatcattgcc gttgctgtag tcaaccgcaa caagagacgt
     4441 ttcatcgctt tccctttccc ttctcggtcg actgtgcctg cggcgcaccc gaagggcgca
     4501 cctcgcccgg cgaaccggac agcgtgcgac cgggcggcaa cgtcgttcca ggtgccgcgg
     4561 gttgcggttg ctgttcgccc ttcggccgca ggttctcgtc ggcccatttg aagtacgcga
     4621 tgaactccc
//
LOCUS       DASSVX010000352         8584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1032067, whole genome shotgun sequence.
ACCESSION   DASSVX010000352 DASSVX010000000
VERSION     DASSVX010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8584
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032067"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VFO53_15005"
     CDS             complement(<1..481)
                     /locus_tag="VFO53_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET8877636.1"
                     /translation="MSEHQRAIEFPEAFLWGSATSAYQVEGSPLADGAGPSIWQRFTH
                     TPGLVRDGDTGDQACDQYRRYADDVALMKRLGMNAYRFSISWSRVLPQGRGAVNAKGL
                     DHYERLVDTLLANAIAPAPTLFHWDLPAALDDRGGWLNPDIAHWFADYASLVFRRLDD
                     "
     gene            complement(532..3699)
                     /locus_tag="VFO53_15010"
     CDS             complement(532..3699)
                     /locus_tag="VFO53_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET8877637.1"
                     /translation="MRIALAVVLVLAASLAHAQPPASDTRILDRFDDVAAWKAVASDG
                     VSASIARVIEAKRPAMRLDFDLGGTAGYAIARRALPLDLPENYAITFWMRADAPVNDL
                     QVKLVDASGDNVWWYDRPNFVFPGEWRQITIKKRQIEFAWGPAKDKALRHVATIEIAI
                     AAGSGGGRGSVYVSDLSLQELPSASAPWPPVKVRASSSQPGAAAARAVDANTKTAWRS
                     KAGPGREQWLSLDFGRPREFGGLVLTWGRNEYASRYDVQFSDDGRKWRTVRRITDGGG
                     GRAALLLTESETRYLRLVLHEGPRREYALAELAVENLEFGASPNAFFEALARDAPRGH
                     FPRGFTGEQGAWTLVGVDGGRDTGLLSEDGALEIGKSGFTLEPFVVERGKVITWADVT
                     ARPFLVDDDLPMPGVQWTAPHWSLRVTTFATGTPDDIHLVARYDVTNLTDQPLALTLA
                     LAARPFQVNPPAQFLNTIGGASPIHDLAWEGGALVVNGTRRVYPLAAPARVGLRPFDA
                     GPLVPWLATTERRAQHDVHDEFGYASGSLFFPATVAAHQTSTFGVVIPLTGDAAAPVL
                     GERTPAQWLFREQARVAATWRAKLDRVTIRVPEEAQPLADALHTALAHILVSRDGPVL
                     RPGTRAYARSWIRDGTMIAESLLRLGQAPVAADYLRWYAPHQFANGKMPCCIDARGAD
                     PVPENDSAGEFIFLAGEVYRYTHDRALLEAMWPHVEKAAAYMGTLRRSERVGAAQDGE
                     RRATYGMLPASISHEGYSAKPMHSYWDDFWALKGYTSAVNIADALGRGDDARQLTAAR
                     DEFGHDLVASLQATAQSHHIDYLPGSVELGDFDPTSSTIALAPAGDERFLPRALVAPT
                     FERYWHEFVARRDGTAPWKEYTPYELRNVAAFVRLGWRERAHELLNFFMEGRRPAAWN
                     QWPEVVGRDPRAVRFIGDLPHAWVASDYIRSVLDLFAYERGDGAMVLAGGVPAPWLDR
                     AGIAIRGLRTPYGTLNYSLVRISDRFAELTLTASGRVPPGGFVLAGPWSRPLHATING
                     KSTTVRGDEVRIDELQARVTLELPR"
     gene            complement(3696..4517)
                     /locus_tag="VFO53_15015"
     CDS             complement(3696..4517)
                     /locus_tag="VFO53_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET8877638.1"
                     /translation="MIPRPAAVVVNGLLAGAAAIALFPLLWMLAVSFMSPGEASAYPP
                     PLVPAHPTFANYRELFAHAGIGRYLANSVLLAAAATALSLVFNVMAGYAFAKLRFRGR
                     EQLFKLMLGALVIPGQVAMVPLFLLLKHLGLVNTYGGVIVPALASIFGIFLVRQYALS
                     IPDDLLEAARIDGASEWRIFGAIVVPLLSPIIVTLAVFTLLGTWNDFMWPLIVLSDQH
                     LYTLPVALASLSREHVQDNELMMAGSVLTTLPVLVVFLVLQRYYIQGLMMGGVKG"
     gene            complement(4646..5566)
                     /locus_tag="VFO53_15020"
     CDS             complement(4646..5566)
                     /locus_tag="VFO53_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8877639.1"
                     /translation="MQASGRAGFVRTLADRRHVAWWFAGPALLVIAVFFVLPVVAALA
                     MSLTDFDIYALADLSNLRVVGLRNYVELLQRPLFWKALGNTLYFVGAGVPLSLGASLC
                     AALLLNSRLARFQPFFRTALFAPVVTTVVAVAVIWRYLLGTRYGIVNYALGAIGVPAI
                     DWLGDPRFAMPAIILFAVWKNFGYNMIILLAGLQNIPNELYEAARIDGASAFARFRFV
                     TLPMLAPILTIVAILTVAGYFQLFAEPYVMTQGGPLQSTVSVLYFMYEEGFKWWNLGN
                     ASAVAFLLFLLIFAVTLLQTRQLRRGGFAP"
     gene            complement(5571..6818)
                     /locus_tag="VFO53_15025"
     CDS             complement(5571..6818)
                     /locus_tag="VFO53_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET8877640.1"
                     /translation="MLAGAGVVFAACSGPAPDVTVVRFWAMGREGEVVTALLPEFERT
                     HPGIRVVVQQLPWTAAHEKLLTAFAGEATPDIAQLGNTWIPEFVALGALQPLDASIRA
                     SSVDPHDFFAGIWDTNVVAAQTYGVPWYVDTRLLFYRRDVLEAAGIASVPVSWADWTR
                     TLGAVKAHADPGRYAILLPMNEVEPLLALALQQPDSLLRDGGRYGNFESAGFRRALDF
                     YVDMFRRGYAPPVTAAQLSNVWNEFGRGTFAYYISGPWNIGEFERRLPPALQSTWATA
                     PLPGPDGPGASIAGGSSLVVFRASKVKDAAWQLVEYLARPEVELRFRELTGDLPPRRS
                     AWSDPALAGDVHARAFREQLERVKPAPKVPEWERIADEMRLVAERAARGEWTVDEAVR
                     ELDARADRILEKRRWLLAREARD"
     gene            complement(6985..8490)
                     /locus_tag="VFO53_15030"
     CDS             complement(6985..8490)
                     /locus_tag="VFO53_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HET8877641.1"
                     /translation="MTSHSIRWRFIVVALFVAALGMLAAASLDDRAVAYGSNMPLGYG
                     ALHHETMPPHGSPLVEPILIDLEERTFRFFWDTANPKNGLVPDRYPTPSFASIAAVGF
                     GLTSYPIGVERGYVTRTEARKRVLATLRFLYNAPQGTHQHGMSGYKGFFYHFLDMKTG
                     ERFDDSELSTVDTALLLAGALFCQSYFDGAHPEEVEIRQLVDQIYRRVDWTWAQPHAP
                     AISLGWSPEDGFLEYDWRGYNEAMLVYVLALASPTHPVGTDAWQQWTSTYDASWSTSF
                     GQEYLSFAPLFGHQYSHVWLDFREVQDAYMKRRGLDYFENTRRAIYAQREYAISNPLR
                     CKDYGASVWGITASDGPGDIEIDDSAGKRVFRAYAARGIGGAAAHDDCTLAPTAAVAS
                     IPFAPELAIPAVVNMYRQFGRYIYGTYGFLDAFNPSFTFADEPLSHGRCIPGFGWVDE
                     DYLGIDQGALVAMIENYRSGLIWDVMRGNTYVRRGLERAGFSGGWLTASAK"
BASE COUNT         1505 a         2864 c         2940 g         1275 t
ORIGIN      
        1 ggtcgtcgag ccttcgaaac acgagcgagg cgtagtcggc gaaccagtgc gcaatgtcgg
       61 gattgagcca gccgccgcga tcgtcgaggg ctgccggaag gtcccagtga aacagcgtcg
      121 gtgcaggcgc gattgcgttg gcgagcaacg tatcgacgag ccgctcgtaa tggtcgagcc
      181 ccttcgcgtt gacggcaccc cggccctgtg gcagcacgcg gctccacgaa atgctgaagc
      241 gataggcatt catgccgagg cgcttcatca gcgcgacatc gtcggcatag cggcggtatt
      301 gatcgcacgc ctggtcgccg gtatcgccgt cgcgcacgag acccggcgtg tgcgtgaagc
      361 gctgccagat gctgggcccg gcgccgtcgg caagcggcga gccttcgacc tggtaggccg
      421 acgtcgcgct gccccagaga aaggcttccg gaaattcgat cgcgcgttgg tgttcgctca
      481 ttgcgtttcc cgaaattcgg aactgatcgg ctccgcgcgg ccaacgttgc ttcagcgcgg
      541 caattcgagg gtgacccgcg cctggagctc gtcgatgcgg acctcgtcgc cacgcacggt
      601 cgtcgacttg ccgttgatcg tcgcatgcaa cgggcgcgac caggggcccg cgagcacgaa
      661 cccgccgggc ggaacgcgac cgctcgcggt cagcgtcagc tcggcaaagc ggtcgctgat
      721 gcgaacgagc gaatagttga gcgtcccgta cggggtgcgc aacccgcgaa tggcaatccc
      781 ggcgcgatcg agccaggggg cgggcacacc gcccgcgagg accatggcgc cgtcgccacg
      841 ctcataggcg aacaggtcga gcaccgagcg gatgtaatcg gacgccaccc atgcgtgcgg
      901 aaggtcgccg atgaagcgca ccgcgcgcgg atcgcgtccg accacttccg gccactgatt
      961 ccacgccgcc gggcgccggc cttccatgaa gaaattcagc agctcatgcg cgcgttcgcg
     1021 ccagccgagg cgcacgaacg ccgcgacatt gcgaagctcg tacggcgtgt attccttcca
     1081 cggcgccgtg ccgtcgcggc gcgcgacgaa ttcgtgccag tagcgctcga acgtcggagc
     1141 aacgagtgcc cgcggaagga aacgctcgtc gccggccggc gcgagcgcga tcgtcgacga
     1201 cgtgggatcg aagtcgccga gctcgaccga gccgggcagg tagtcgatgt ggtggctttg
     1261 cgccgtcgct tgcagcgacg cgacgagatc gtgaccgaac tcgtcgcgcg ccgcggtgag
     1321 ctggcgcgcg tcgtcgccgc ggccgagcgc gtcggcgatg tttaccgcgc tcgtgtagcc
     1381 cttgagcgcc cagaagtcgt cccagtacga gtgcatcggt tttgccgagt agccctcgtg
     1441 gctgatcgat gcgggcagca tgccgtaagt cgcgcgacgt tcgccgtcct gcgccgcgcc
     1501 gacgcgctcg cttcgccgca gcgtgcccat gtacgccgca gccttctcga cgtgcggcca
     1561 catcgcctcg aggagcgcac ggtcgtgcgt atagcgatac acctcgccgg cgaggaagat
     1621 gaactccccg gcgctgtcgt tctcgggaac gggatcggcg ccgcgcgcgt cgatgcagca
     1681 gggcatcttg ccgttggcga actggtgcgg cgcgtaccag cgcaggtaat ccgccgcgac
     1741 cggcgcctgg ccgaggcgca gcaacgactc ggcgatcatc gtgccgtcgc gaatccacga
     1801 gcgcgcgtac gcccgcgttc ccggacgcag caccgggccg tcgcgcgaga cgagaatgtg
     1861 cgccagcgcc gtgtgcagcg cgtcggcgag cggctgcgct tcctcgggca cgcggatcgt
     1921 cacgcgatcg agcttcgcgc gccacgtcgc cgcaacgcgc gcctgttcgc ggaaaagcca
     1981 ctgtgcgggc gtgcgttcgc cgaggacggg ggctgcggcg tcacccgtga gcggaatcac
     2041 gacgccgaac gtcgacgtct gatgcgccgc gaccgtcgcg ggaaagaaaa gcgagcccga
     2101 tgcgtacccg aactcgtcgt gcacgtcgtg ctgcgcgcgc cgctcggtgg tggcgagcca
     2161 cggcacgagc ggccccgcgt cgaagggtcg caggccgact cgcgccggtg cggcgagcgg
     2221 gtacacgcga cgcgtgccat tgaccacgag cgcgccgccc tcccaggcga gatcgtggat
     2281 cgggctcgcg cccccgatgg tattcagaaa ttgcgccggc gggttgacct ggaacggccg
     2341 cgccgcaagc gcgagcgtga gtgcgagcgg ctgatcggtg agattggtga cgtcgtagcg
     2401 ggcgacgaga tggatatcgt ccggcgtgcc ggtcgcaaac gtcgtgacac gcagcgacca
     2461 gtgcggggcc gtccattgca cgcccggcat cggcaggtcg tcgtcgacga ggaacgggcg
     2521 cgcagtgacg tcggcccacg tgatgacctt gccgcgctcg acgacgaacg gctcgagcgt
     2581 gaacccgctc ttgccgatct cgagcgcacc atcttccgac aagagcccgg tatcgcgacc
     2641 gccgtcgacg ccgaccagcg tccacgcacc ctgctcgccc gtgaatccac gtggaaagtg
     2701 gccgcgcggc gcatcgcgtg cgagcgcttc gaagaacgca ttgggcgacg cgccgaactc
     2761 gagattctcg accgcaagct cggcgagcgc gtattcgcgc cgcgggcctt cgtgcagcac
     2821 gagccgcagg tagcgcgtct ccgattcggt caaaagcagc gccgcgcgtc cgccgccgcc
     2881 gtcggtgatg cggcgcacgg tgcgccattt gcggccgtca tcggaaaact gcacgtcgta
     2941 gcgtgacgcg tattcgttcc tgccccaggt cagcacgagg ccgccgaact cgcgcggccg
     3001 tccgaaatcg agactcagcc attgctcgcg accgggcccc gccttgctgc gccacgcggt
     3061 cttcgtgttt gcgtcgacgg cgcgcgcggc cgcggcaccc ggctgcgacg atgacgcgcg
     3121 caccttcacg ggcggccacg gtgccgatgc ggacggcaat tcctgcagcg acaggtcgct
     3181 cacgtagacc gagccgcggc caccgccgct tccggcggcg atggcgatct cgatcgttgc
     3241 aacatggcgc aacgccttgt ccttcgcggg tccccaggcg aactcgatct gccgcttctt
     3301 gatcgtgatc tgacgccatt cgccggggaa aacgaagttc ggccggtcgt accaccagac
     3361 gttgtcgccg ctcgcgtcga cgagcttcac ctggaggtcg ttgacgggcg catcggcacg
     3421 catccagaac gtgatcgcgt agttctcggg caggtcgagc ggcagcgccc ggcgcgcgat
     3481 cgcgtagccc gcggtgccgc cgagatcgaa gtcgagacgc atcgccggac gcttcgcctc
     3541 gatgacgcgc gcgatcgacg ccgacacacc gtcggacgca accgccttcc atgcggccac
     3601 gtcgtcgaag cgatcgagga tgcgcgtgtc ggacgccggc ggctgcgcgt gcgcgagcga
     3661 cgcggcgagc acgagcacga ccgcaagcgc gattctcatc ccttcacacc gcccatcatc
     3721 aatccctgga tgtagtagcg ctgcagcacg aggaacacga cgagcaccgg cagcgtcgtc
     3781 agcaccgagc cggccatcat cagctcgttg tcctgtacgt gctcgcgcga aaggctcgcg
     3841 agcgcgaccg gcagcgtata caggtgctgg tccgacagta cgatcagcgg ccacatgaaa
     3901 tcgttccagg tgccgagcag cgtgaacacg gcgagggtga cgatgatcgg tgaaagcagc
     3961 ggcacgacga tcgcgccgaa gatccgccat tcgcttgcgc cgtcgatgcg cgccgcttcc
     4021 agcaggtcgt cgggaatcga cagcgcgtac tggcgcacga gaaagatgcc gaagatgctc
     4081 gcgagcgcag gcacgatcac gccgccatac gtattgacga gcccgaggtg cttgagcagc
     4141 aggaacaggg gcaccatcgc cacctgcccg ggaatgacca gtgcgcccag catcagcttg
     4201 aagagctgct cgcgaccgcg aaagcgcagc ttcgcgaatg cgtaaccggc catcacgttg
     4261 aagaccagcg acaatgcggt cgccgcagcg gcgagcagca cgctgttcgc aagatagcgc
     4321 ccgatgcccg cgtgcgcgaa gagctcgcgg tagttggcga acgtcggatg cgcagggacg
     4381 agcggcggcg gataggcgct cgcctcgccg ggggacatga acgacaccgc gagcatccac
     4441 agcagcggga acagcgcgat ggccgccgcg ccggccagca gtccgttgac gacgaccgcg
     4501 gcgggacgcg gaatcacgcg ccatgcccag ggcctggccg ctgcggacac cgcagcgctc
     4561 accgaaccac cctccggcgc gctcgttacg accctccgca cttcgtgctg cggggcgatt
     4621 cgcgcttggg accggcccgg cgcgctcatg gcgcgaaccc gccgcggcgc aactggcgcg
     4681 tctgcagcag cgtgactgcg aagatcagca ggaacagcag gaacgccacc gccgacgcgt
     4741 tccctaggtt ccaccatttg aagccttcct cgtacatgaa atacagcacg ctgaccgtgc
     4801 tctgcagcgg gccgccctgc gtcatcacgt acggctcggc gaagagctgg aaatagccgg
     4861 cgacggtcag gatcgcgacg atggtgagga tcggcgcgag catcggcagc gtgacgaagc
     4921 ggaagcgtgc gaatgccgat gcgccgtcga tgcgcgccgc ttcgtaaagc tcgttcggga
     4981 tgttctgcaa tccggcgagc aggatgatca tgttgtagcc gaagttcttc cacacggcga
     5041 acaggatgat ggccggcatc gcgaagcgcg gatcgccgag ccagtcgatc gcgggaacgc
     5101 cgatcgcacc gagcgcgtag ttgacgatgc cgtaacgcgt gccgagcaga tagcgccaga
     5161 tcaccgcgac ggcgacgacc gtcgtcacga ccggcgcgaa aagcgccgtg cgaaagaacg
     5221 gctggaagcg cgcgagcctc gaattgagca gaagcgccgc gcaaagcgat gcgccaagcg
     5281 acagcggcac gcccgcgccg acgaagtaaa gcgtgttgcc gagcgctttc cagaaaagcg
     5341 gccgctgcag cagctcgacg tagttgcgca gcccgaccac gcgcaggttc gaaaggtcgg
     5401 cgagcgcgta gatgtcgaag tcggtgaggc tcatcgccag tgcggcgacg acgggcagca
     5461 cgaagaacac ggcgatgacg agaagcgcgg gtccggcgaa ccaccaggcg acatggcgtc
     5521 gatcggcgag cgtgcggaca aaacccgcac gcccgcttgc ctgcagcgcg ctagtcacgc
     5581 gcttcgcgcg cgaggagcca ccggcgcttc tcgaggatgc gatcggcgcg cgcgtcgagc
     5641 tcgcgcaccg cctcgtcgac cgtccattcg ccgcgcgcag cgcgctcggc gacgaggcgc
     5701 atttcgtcgg cgatgcgttc ccactcgggc accttcggcg cgggcttcac gcgctcgagc
     5761 tgctcgcgga aggcgcgcgc gtgcacgtca ccggccagcg cgggatcgct ccatgcgctg
     5821 cgccgcggcg gcaggtcgcc ggtcagctcg cgaaagcgaa gctcgacctc ggggcgggcg
     5881 agatactcga ccagttgcca cgccgcgtcc ttgaccttcg acgcgcgaaa gaccacgagg
     5941 ctcgatcctc cggcgatcga cgcacccggg ccgtccggtc cgggcagcgg cgccgtcgcc
     6001 cacgtcgatt gcaacgccgg cggcaaccgc cgctcgaact cgccgatgtt ccacggcccc
     6061 gaaatgtagt acgcgaacgt gccgcgtccg aattcgttcc acacattcga cagttgcgcc
     6121 gcggtgaccg gcggcgcgta gccacgacga aacatgtcga cgtaaaagtc cagcgcgcgg
     6181 cgaaagccgg cgctttcgaa attgccgtag cggccgccgt cgcgcagcag cgaatcgggc
     6241 tgctgcagcg ccagcgcgag caggggctcg acttcgttca tcggcagcag gatcgcatag
     6301 cgcccgggat cggcgtgcgc cttgaccgcg ccgagcgtgc gcgtccaatc ggcccacgac
     6361 acgggcacgg atgcgatgcc cgccgcttcg agcacgtcgc gccggtagaa cagcagccgc
     6421 gtgtcgacgt accagggcac gccgtaggtt tgcgctgcga cgacgttggt gtcccagatg
     6481 cccgcgaaga aatcgtgggg atccaccgag gatgcgcgaa tcgacgcatc gagcggttgc
     6541 agcgcgccga gggcgacgaa ttcggggatc cacgtgttgc cgagctgcgc aatgtcgggg
     6601 gtcgcttcgc ccgcgaacgc ggtcagcagc ttttcgtgcg cggccgtcca cggaagctgc
     6661 tgcacgacga cgcgaatgcc cgggtgcgtg cgctcgaact cgggcagaag tgcggtgacg
     6721 acttcgcctt cgcgtcccat cgcccagaag cgcacgacgg tgacatcggg cgccggcccg
     6781 ctgcacgcag cgaaaaccac gcccgcaccg gcgagcacga gcgcccataa aatgaccctc
     6841 cgcgctccgc gcttcggggc caattcgcgc ttgggtcggc ccggcgcgct cattgcacga
     6901 ccctccgcgc tccgcgcttc ggggccaatt cgcgcttggg tcggcccggc gcgctcatgc
     6961 agcagcgccg gcaccgtatg ccggtcactt cgccgatgcg gtgagccagc cccccgagaa
     7021 gccggcacgc tcgaggccgc ggcgcacgta cgtgttgccg cgcatgacgt cccagatcag
     7081 gccgctgcga tagttctcga tcatcgcgac gagcgcaccc tgatcgatgc cgaggtagtc
     7141 ttcgtcgacc catccgaagc ccggaatgca gcgcccgtgg ctcaacggct cgtcggcgaa
     7201 ggtgaagctc gggttgaatg catcgagaaa gccataggtg ccgtagatgt agcggccgaa
     7261 ttgccgatac atgttgacga cggcggggat cgcgagttcc ggggcgaacg gaatcgacgc
     7321 gacggcggcc gtcggcgcga gcgtgcaatc gtcgtgcgcg gctgcgccgc cgatgccacg
     7381 cgccgcgtag gcgcgaaaga cccgcttgcc ggcgctgtcg tcgatctcga tgtcgccggg
     7441 gccgtcgctt gccgtgatgc cccacacgct cgcgccgtaa tccttgcagc gcagcggatt
     7501 ggagattgcg tattcgcgct gcgcgtagat cgcccggcgg gtgttctcga agtagtcgag
     7561 gccgcgccgc ttcatgtacg cgtcctgcac ctcgcggaaa tcgagccaca cgtgcgaata
     7621 ctggtgaccg aagagcggcg cgaaggaaag gtattcctgg ccgaagctcg tgctccagct
     7681 tgcgtcatac gtgctcgtcc attgctgcca ggcatcggtg cccaccggat gcgtcggcga
     7741 cgcgagtgcc agcacataga cgagcatcgc ctcgttgtag ccgcgccagt cgtattcgag
     7801 gaagccgtcc tcgggcgacc agccgaggct gatcgccggc gcgtgtggct gcgcccacgt
     7861 ccagtcgaca cggcgataga tctggtcgac gagctggcgg atctccacct cttcgggatg
     7921 cgcgccgtcg aagtacgact ggcagaacag cgcgccggcg aggagaagtg cggtgtcgac
     7981 cgtcgacaac tcgctgtcgt cgaagcgctc gccggtcttc atgtcgagga aatggtagaa
     8041 gaaacccttg tagcccgaca tgccgtgctg gtgcgtcccc tgcggcgcgt tgtacaggaa
     8101 gcgcagcgtc gcgagcacgc gcttgcgcgc ctccgtgcgc gtgacgtagc cgcgctcgac
     8161 gccgatcgga tacgacgtga gcccgaatcc gacggcggcg atgctcgcga acgacggcgt
     8221 cgggtagcga tcgggaacga ggccgttctt cggattggcg gtgtcccaga agaagcggaa
     8281 cgtgcgctcc tcgagatcga tcaggatcgg ctcgacgagc gggctgccgt ggggcggcat
     8341 tgtttcgtgg tgcaatgcgc catagccgag cggcatgttg ctgccgtacg cgactgcgcg
     8401 atcgtcgagc gatgcggcgg cgagcatgcc gagcgccgcg acgaacagcg cgacgacgat
     8461 aaagcgccag cggatcgaat ggctggtcat gtcattgtca tttccggatc cggcgaagaa
     8521 taccggaaat gtcgctcgca agcgacatcg acgaattgat gtttcgttca aaaacgataa
     8581 atca
//
LOCUS       DASSVX010000353         5899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1565898, whole genome shotgun sequence.
ACCESSION   DASSVX010000353 DASSVX010000000
VERSION     DASSVX010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565898"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="VFO53_15035"
     CDS             complement(<1..1481)
                     /locus_tag="VFO53_15035"
                     /inference="COORDINATES: protein motif:HMM:NF028457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HET8877642.1"
                     /translation="MLHRHLSRASLAVGILAPFVMSGCASLPQANAAKPADVAAIAAA
                     AVASAEARDEPPGNGHAEAPGGSRSGAAAAAATAAAQAAQYSKPFAEVVKEAKETPGL
                     FNIWQKDDKVWLEIGPDQFDHPFFFKSAVNQGIGEHRIFGGAMTYPIGVAQIVVFHKH
                     GQTVQLIAKNVKYTAKPGTPEARAVEAGFSDSLLAVAPIASQPSQERKSVLIDANALL
                     LADIPAGAAMLDRTYHQSYGFDARNSSIGITHGQSDNVTLEVSAHYAVGRLVVPGPGH
                     PPGAQQPSQPTTLPDPRSLFIGYHYTFAKLPDEPMHPRPADERVGYFTTEVLDFTSDV
                     PRVPVERYANHWRLEKKDPSAALSEPKQPIVFWLDRNIPVKYREPIKEGILEWNKAFE
                     RIGFKDAIHVEVQPDDADFDTSDIHHASVRWQTVAKTSYGAIGPSVVDPRTGEILDAD
                     IGIDANNVRVVRNLRHEYLPPRKDAFAAFAEATSGNAHAQSDA"
     gene            complement(1550..2965)
                     /locus_tag="VFO53_15040"
     CDS             complement(1550..2965)
                     /locus_tag="VFO53_15040"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HET8877643.1"
                     /translation="MASKLFIRTFGCQMNEYDSDRMADVLAREGLTLTDRPDDADVIL
                     FNTCSVREKAQERVFHDLGRVRALKVERPDVLIGVGGCVASQEGAAIVRRAPYVDLVF
                     GPQTLHRLPAMIRARRESGAPQVDISFPEIEKFDHLPPPRVDRASAFVSIMEGCSKYC
                     SFCVVPYTRGEEVSRPFDDVLTELADLALSGVKEITLLGQNVNAYRGPIDVRGEHMSA
                     AGPSQGAHSPSGGSAAAKPHAWGGQYADLATLLEYAADIPGIERLRYTTSHPKEMTSR
                     LIDAYATIDKLVSHLHLPVQSGSDRVLAAMKRGYTAIEYKSTVRKLRARRPDLSLTSD
                     FIVGFPGETDDDFEKTLRLVEDVAFDGAFSFAYSPRPGTPAADLRDQVPVEVKQARLA
                     RLQALLDRQYRDNSDRMVGTRQRVLVTGTAVRNPRELAARTDNNRVVNFAGDPAAIDC
                     YVDVDITAALPHSLRGELTLA"
     regulatory      3005..3178
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3404..5269
                     /locus_tag="VFO53_15045"
     CDS             3404..5269
                     /locus_tag="VFO53_15045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET8877644.1"
                     /translation="MSPLATAPSRASSPWWLVPCIAALSGTSYAQPVSPAVLDPIVVT
                     ASRSPQRLLDLVADVTVIDADEIARGGAQGLVALLSRQPGVEIIQNGGPGATSGVFLR
                     GANAAQTLVLIDGMRVASASSGAAALEAIPVEQIERVEILRGPASSLYGADAIGGVIQ
                     VFTRRGPGGTGGIAANASIGYGTFDTWSGAAGLSGGRGPLHGSVELAGRGSEGFNAIV
                     NPANPSYDPDRDGYRNASVSAQGSLDAAAGQTLALQYFKSRLDDRFDAGDGFDDRTMT
                     TLETWQIASINQIARNWASRLSFGDGSDRSVSRTGFGEFPFDTHQRQLAWQNDFTLPQ
                     GALTAALERREERVGAGAGFPVTGRDTNAITAIYRVDAGANALQANVRHDDSNQFGGK
                     TTGALAWGYRLTPAWRVTASVGTAFKVPTFNDLYFPGFSNPDLRPESSRNVEAGAHWT
                     SRAGEFAWQAHAVAFHQRVDDLIVFQCDASFNCAPNNVADATLEGASFYGDVEWRDTL
                     VHASLDLQSPTDTASGLLLPRRARRHGVVSLAQALGRARVSAEIVGSSARFDDAENRR
                     RLGGYGVVNVAVEWTLDASTTLFVRADNVLDHHYELAADFATGGARVFGGMRWRL"
     gene            5266..>5899
                     /locus_tag="VFO53_15050"
     CDS             5266..>5899
                     /locus_tag="VFO53_15050"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HET8877645.1"
                     /translation="MTRFRTLALLAMLVAAPAVAAVRVVDDTGGVVELASPATRIVTL
                     APHAAELVFAAGAGARIVGVIKGSDHPPALRTRPVVGDVAALDVERIVALAPDLIVTW
                     PWTTPRQVAALRERGIAVFEADARSIDAIAGDLERIGRLAGTADIASAAARTFRARVV
                     AAASERTPGPPMRVFYEVSDVPLFTLGGTHLVSQALALCGGENVFARLSIP"
BASE COUNT          905 a         1955 c         2068 g          971 t
ORIGIN      
        1 tcggcgtcgc tctgcgcatg cgcattgccg ctcgtcgctt cggcgaacgc agcgaaggca
       61 tccttgcgcg gcggcaggta ttcgtggcgc aggttgcgca cgacgcgcac gttgttcgcg
      121 tcgatgccga tatcggcatc gaggatctcg ccggtgcgcg gatcgacgac cgacgggccg
      181 atggccccgt acgacgtctt ggccaccgtc tgccagcgca ccgacgcgtg atggatgtcg
      241 gaggtgtcga aatccgcgtc gtcaggctgg acctcgacgt gaatcgcatc cttgaagccg
      301 atgcgctcga acgccttgtt ccattcgagg atgccttcct tgatcggttc gcggtacttg
      361 accgggatgt tgcggtcgag ccagaacacg atcggctgct tcggctcgga cagcgcggcc
      421 gacgggtcct tcttctcgag ccgccagtga ttggcatagc gctcgaccgg cacacggggg
      481 acgtcggagg tgaaatcgag cacttcggtc gtgaaataac cgacgcgctc gtcggcgggt
      541 cgcgggtgca tcggctcgtc gggcagcttc gcgaacgtgt agtgatagcc gatgaagagg
      601 ctgcgcgggt cggggagcgt cgtcggctgc gacggctgct gcgcgccggg cggatggccg
      661 ggtcccggca cgacgagacg cccgacggcg taatgcgcgg acacctcgag cgtcacgttg
      721 tcggattgcc cgtgcgtgat gccgatcgaa gagtttcgcg catcgaagcc gtacgactga
      781 tggtaggtgc ggtcgagcat cgccgcgccg gccgggatgt cggccagcag aagcgcgttc
      841 gcgtcgatca gcaccgattt gcgctcttgc gatggctgcg acgcgatcgg cgccacggcg
      901 agcaggctgt cggagaatcc ggcctcgacc gcgcgcgcct cgggtgtgcc cggcttggcc
      961 gtgtacttga cgttcttcgc gatgagctgg accgtctggc cgtgcttgtg gaacacgacg
     1021 atctgcgcga cgccgatcgg gtaggtcatc gcgccgccga aaatgcggtg ctcgccgatg
     1081 ccctggttca ccgccgactt gaagaagaac gggtggtcga actgatcggg gccgatctcg
     1141 agccacacct tgtcgtcctt ctgccagatg ttgaacagac ccggggtctc cttcgcctct
     1201 ttgacgacct cggcgaacgg cttcgaatat tgcgccgcct gcgcggcggc ggtggccgcc
     1261 gcggccgccg cgccggaacg tgacccgccc ggcgcttccg cgtggccgtt gcccgggggc
     1321 tcgtcgcggg cctcggcgct tgcaacggcg gcggcggcga tggcagccac gtcggcaggc
     1381 ttcgcggcat tcgcctgcgg aaggctcgcg cagccggaca tcacgaacgg tgcgaggatg
     1441 ccgacggcaa ggctcgcgcg ggaaagatga cgatgcaaca tgaacgaggt gtctccagga
     1501 tcgacgaggg gtcgtgcgcc ggtcgcaaca gcgacgcgtc gaagaccgtt cacgcgagcg
     1561 taagttcgcc gcgcaatgag tgcggcagcg ccgccgtaat gtcgacgtcg acgtagcagt
     1621 cgatcgccgc ggggtcgccc gcgaaattga cgacccggtt gttgtctgtg cgcgcggcca
     1681 gctcgcgcgg gttcctgacg gcggttccgg tgacgagcac gcgctggcgc gtgccgacca
     1741 tccggtcgct attgtcccga tactggcggt cgagcaacgc ctgcaatcgc gcaaggcgcg
     1801 cctgcttcac ctcaaccggg acctggtcgc gaagatcggc ggccggcgtg ccgggacgcg
     1861 gactgtacgc gaagctgaag gcgccgtcga acgccacgtc ctcgactagg cgcagcgtct
     1921 tctcgaaatc gtcgtcggtc tcgccgggga agccgacgat gaaatcggac gtgagcgaaa
     1981 ggtcgggccg gcgcgcccgc agcttgcgca ccgtggactt gtactcgatt gccgtgtatc
     2041 cgcgcttcat cgccgcgagc acgcggtcgg aacccgactg caccgggagg tggagatgcg
     2101 agacgagctt atcaatcgtc gcgtacgcat cgatcagccg cgaagtcatc tccttcggat
     2161 gcgacgtcgt gtagcgcagc cgctcgatgc ccgggatgtc cgccgcgtat tcgagaagcg
     2221 tcgcgagatc ggcatattga cccccccacg catgcggctt cgccgctgcg ctgccccccg
     2281 atggggaatg agcgccttgg gacggccctg cggcgctcat gtgttcaccc cgaacgtcga
     2341 tcggaccgcg ataggcgttg acgttctgtc cgagcagagt gatctccttc acccccgaaa
     2401 gtgccaggtc ggcgagctcg gtcagcacgt cgtcgaacgg ccgcgacact tcctcgccgc
     2461 gggtgtacgg aacgacgcag aacgagcagt acttgctgca gccctccatg atcgagacga
     2521 acgcggacgc acggtcgacg cgcggcggcg gaagatgatc gaacttctcg atctcgggga
     2581 acgaaatgtc cacttgcggc gcacccgatt cgcgccgcgc gcgaatcatc gccggcagcc
     2641 ggtgcagcgt ttgcgggccg aacacgagat cgacgtacgg cgcgcgccgc acgatggcag
     2701 caccttcctg cgaggcgacg cagccgccga cgccgatcag cacgtcgggc cgctcgacct
     2761 tgagcgcgcg cacgcggcca aggtcgtgga acacgcgctc ctgcgccttc tcgcgcaccg
     2821 agcatgtgtt gaagaggatc acgtccgcgt cgtcgggacg atcggtcagc gtcagaccct
     2881 cgcgagcgag cacgtcggcc atgcggtccg agtcgtactc gttcatctgg cagccgaacg
     2941 tgcgaatgaa aagcttgctt gccacagcgg tgtctttgga ggggggcgaa ttataacaaa
     3001 ccacagtaga atcgcgggtg caggtgtccg ccggacggcg ttctgaagcg accggcgggt
     3061 gaaacgggaa tgcggtgccc gcgcatcacg cgcgacatgc cgcagctgcc cccgcaacgg
     3121 tagggaaagt gaacccgctt tccaagcccg gagaccggcc tgcaggccag ggatcagagg
     3181 tcaggcgtcg gcaaccgcgc gtcgcgcgcg tgctgctgcc gtttcccgct ccctggttgc
     3241 gacaaagaac cgtccggaaa cgggttcggc atcgaggttc gggacggcgt cttgcgcgcg
     3301 ctttggcgcc gacgccaatc gctgatcccc gacctctgat ccctgtttcc ggatccctgg
     3361 taagccgtgc ggggcactca cggcatgacg cggaccgatc ccaatgtctc ctctcgcaac
     3421 agcgccgtca cgggcgtcat cgccgtggtg gctcgtccca tgcatcgccg cgctgagcgg
     3481 cacgtcgtac gcgcagcccg tatcccccgc ggtcctcgat ccgatcgtgg tcacggcgtc
     3541 gcgatcgccg cagcggctgc tcgatctcgt ggccgacgtg acggtgatcg acgccgacga
     3601 aattgcgcgc ggcggcgcgc agggcctcgt ggcactcctt tcgcgccagc cgggtgtcga
     3661 gatcatccag aatggcggcc cgggcgccac gtcgggcgtc ttcctgcgcg gcgcaaacgc
     3721 ggcgcagacg ctggtgctga tcgacggcat gcgcgttgcc tcggcgtcga gcggcgcggc
     3781 cgcgctcgag gcgatcccgg tcgagcagat cgagcgcgtc gagatcctgc gcggacccgc
     3841 gtcgagtctt tacggcgccg acgcgatcgg cggggtgatc caggtgttca cgcgtcgtgg
     3901 cccgggcggc acaggcggca tcgcggcgaa cgcgtcgatc ggctacggca cgttcgatac
     3961 ctggtcgggc gcggcgggcc tttcgggcgg acgcggcccg ctgcacggca gcgtggagct
     4021 cgcgggccgc gggagcgagg gcttcaacgc gatcgtcaat ccggccaatc cgagctacga
     4081 ccccgatcgc gacggctacc gcaacgcgag tgtcagcgcg cagggctcgc tcgatgccgc
     4141 ggcgggccag acgctggcgc tccagtattt caagagccgc ctcgacgatc ggttcgatgc
     4201 gggcgacggc ttcgacgatc gcacaatgac gacgctcgaa acgtggcaga tcgcatcgat
     4261 caaccagatt gcgcgcaatt gggcttcgcg actgtcgttc ggcgacggaa gcgaccgctc
     4321 ggtatcgaga accgggttcg gcgagttccc gttcgatacg catcagcgcc agctcgcgtg
     4381 gcagaacgat ttcacgctgc cgcagggcgc gcttaccgcg gcgctcgagc ggcgcgagga
     4441 gcgcgtcggt gccggcgccg gctttccggt caccggacgc gacacgaatg ccatcaccgc
     4501 aatctaccgc gtcgacgccg gcgcgaatgc actgcaggcg aatgtgcgcc atgacgattc
     4561 gaaccagttc ggcggcaaga ccaccggcgc actggcgtgg ggctatcggc tcacgccggc
     4621 gtggcgcgtg acggcgagcg tcggcacggc cttcaaggtg ccgacattca acgacctgta
     4681 tttccctggt ttctcgaatc cggacctgcg gcccgaatcg tcgcgcaatg tcgaggcggg
     4741 cgcgcactgg acctcgcgcg ccggcgaatt cgcatggcag gcgcatgcgg tcgcgtttca
     4801 tcagcgcgtg gacgacctga tcgtcttcca gtgcgacgcg agcttcaact gcgcaccgaa
     4861 caacgtcgcc gatgcgacgc tcgaaggtgc aagcttctac ggcgatgtcg aatggcgcga
     4921 cacgctcgtg cacgcgtcgc tcgacctgca atcgcccacc gacacggcca gcggcctcct
     4981 gctgccgcgg cgcgcgcgcc gccacggcgt ggtgtcgctc gcgcaggcgc tcggccgggc
     5041 gcgcgtcagc gccgagatcg tcggctcgtc ggcgcgcttc gacgacgcgg agaatcggcg
     5101 ccgcctcggc ggttacggcg tcgtcaacgt cgccgtggag tggacgctcg acgcgtcgac
     5161 gacgctgttc gttcgcgccg acaacgtgct cgatcaccac tacgagctgg ccgccgactt
     5221 cgcgacgggg ggcgcacgcg tcttcggcgg gatgcggtgg cggctgtgac ccggttccgg
     5281 acgctggcgt tgctcgcgat gctggtcgcg gcgccagccg ttgcggcggt tcgcgtcgtc
     5341 gacgatacgg gcggcgtggt cgagctcgcc tcgcccgcga cgcggatcgt cacgcttgcg
     5401 ccgcatgcgg cggagctcgt gttcgccgcg ggggccggcg cgcggatcgt cggcgtgatc
     5461 aagggcagcg accatccgcc agcgctgcgc acgcgcccgg tcgttggcga tgtcgccgcg
     5521 ctcgacgtcg agcgcatcgt cgctctcgcc cccgacctga tcgtcacctg gccgtggacg
     5581 acgccgcgcc aggtggcggc gctgcgcgaa cgcggcatcg cggtgttcga ggccgatgcg
     5641 cggtcgatcg acgccatcgc gggcgacctc gaacgcatcg gacgccttgc cgggaccgcc
     5701 gatatcgcaa gcgcggcggc gcggaccttc cgtgcacgcg ttgttgcggc tgcgagcgaa
     5761 cgcacgccgg gaccgccgat gcgcgtgttc tacgaggtct ccgatgtacc gctgttcacg
     5821 ctgggcggca cgcatctcgt gagccaggcg cttgcgcttt gcggcggcga gaacgtgttt
     5881 gcgcggctgt cgattccgg
//
LOCUS       DASSVX010000354         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_548970, whole genome shotgun sequence.
ACCESSION   DASSVX010000354 DASSVX010000000
VERSION     DASSVX010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548970"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..633
                     /gene="pal"
                     /locus_tag="VFO53_15055"
     CDS             79..633
                     /gene="pal"
                     /locus_tag="VFO53_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HET8877646.1"
                     /translation="MRKYLWIGLIAAALAGCSTTPSTEAPVDDKSSAGAAGAGAGGAQ
                     TGGAGAGGVTGSALGSGAGGNPLRDPNNILSKRSVYFDYDSFVVKDEYRPLVEAHARY
                     LQQNRNARATVQGNTDERGSREYNIALGQKRADSVKRMMTLLGATESQIETVSFGKEK
                     PKNPGHDESAYAENRRDDILYSGE"
     gene            637..1527
                     /gene="ybgF"
                     /locus_tag="VFO53_15060"
     CDS             637..1527
                     /gene="ybgF"
                     /locus_tag="VFO53_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HET8877647.1"
                     /translation="MKLSLRATLAVVAVLLGVNAAHAAIFDDDEARRRIEATNQRVAQ
                     LQRQLEDRLAVLEQQAKSGGLADLGTQVQLLQSDLAKLRGQIEVITYELEQSQKRQRD
                     LYVDLDSRLRKIESSPALTAPPAAGSSDAAAPGAVPPTAGTLPPAAVPPSTAAGAPAV
                     VATAPPVRNPSDGVAEQRAYDAALDLFKRGDYQGAINGFSAFVKAYPRSPLASSAHYW
                     MGNAQYARRDYRGSIATQRQMLKEFPDSSKAPDALLNIASAQSDLGDNAAARRTLEEL
                     IAKYPKSEAGTRARQRLGGR"
     gene            1535..2596
                     /gene="mutY"
                     /locus_tag="VFO53_15065"
     CDS             1535..2596
                     /gene="mutY"
                     /locus_tag="VFO53_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET8877648.1"
                     /translation="MKTFAARVSEWQRVHGRHDLPWQQDRDPYRIWLSEIMLQQTQVV
                     TVLPYYARFVAAFPTVASLAAAPLDHVLAVWSGLGYYRRAHHLHAAANAVVRDQGGVF
                     PRDAATLAALPGIGRSTAAAIAAFASDERGAILDGNVKRVLARHRGVDGWPGEPRVQA
                     ALWQIADALVPPNASDIRAYTQGLMDIGATICTRTRQQCAVCPIREDCEARLTGRVAE
                     LPAPRPRKVLPQREVTVLMLERDGDVLLERRPVLGVWGGLWSLPELPRDADIASHVGI
                     KLAMMPGELRRLPPLTHTFTHFALTMYPVRVQVARAPLAVRMPGVEWFARDAALAAAI
                     PAPIRKLLQISEAEFRLTG"
BASE COUNT          484 a          959 c         1010 g          525 t
ORIGIN      
        1 gtggggaccg ttcccgcgcc aatgactttt tcgaatgcgt gccgtggtcg cggcgcgccg
       61 acttactgtg gaggtggcat gcgcaaatat ctttggatcg gattgatcgc ggcggcactc
      121 gccggctgct cgacgacacc ctcgacggag gcacccgtcg acgacaagag ctcggcaggc
      181 gccgcgggcg ccggcgcggg cggcgcgcag accggcggcg ccggcgctgg gggcgtgacg
      241 ggctccgcgc tgggatcggg cgcaggtggc aatccgctgc gtgatcctaa caacattctg
      301 tcgaagcgca gcgtctactt cgattacgac agcttcgtcg tcaaggacga gtatcgtccg
      361 ctcgtcgaag cgcacgcgcg ttacctgcag caaaatcgca atgcgcgcgc aacggttcaa
      421 ggcaatacag acgagcgcgg ctcgcgcgaa tacaacatcg cgctcggcca gaagcgtgcg
      481 gattcggtga agcggatgat gacgctgctc ggcgcgaccg aaagccagat cgagacagtg
      541 agcttcggca aggaaaagcc gaagaatccg ggccacgatg aatccgcgta cgcggagaat
      601 cgccgcgacg acattctcta ttcgggcgag taagcgttga agctgtcgtt gcgtgccacg
      661 cttgccgtcg tcgccgttct gctcggcgtc aatgccgcgc atgcggcgat cttcgacgac
      721 gacgaagcgc ggcgacgcat cgaagcgacg aaccagcgcg ttgcgcaact gcagcggcag
      781 ctcgaggatc ggctcgcggt gctcgagcag caggcgaaaa gcggcggcct cgctgacctc
      841 ggcacgcagg tgcagctgct gcaaagcgat ctcgcgaagc tgcgcggcca gatcgaggtg
      901 atcacgtacg agctcgagca gtcgcaaaag cggcagcgcg acttgtacgt cgatctcgat
      961 tcgcggctgc gcaaaatcga gtcttcaccg gcgctgaccg cgccgccggc agcgggatcg
     1021 tcggatgcgg cggcgcctgg cgccgtgcca ccgactgccg gcacgctgcc accagccgcg
     1081 gtgccgccga gcactgccgc aggtgcgcct gccgtcgttg ccaccgcgcc acccgttcgc
     1141 aacccttccg atggtgtcgc cgagcagcgc gcttacgacg ccgcgctcga tttgttcaag
     1201 cgcggcgatt accagggcgc gatcaacggc ttctcggcgt tcgtgaaggc gtatccgcgc
     1261 agcccgctcg catcgtcggc acattactgg atgggcaacg cgcagtacgc gcgccgcgac
     1321 tatcgcggat cgatcgcgac gcagcggcag atgctgaaag agtttcccga cagcagcaaa
     1381 gcaccggatg cgttgctgaa cattgcgtcg gcgcaatccg atctcggcga caacgccgcc
     1441 gcgcggcgca cgctcgagga gctgatcgcg aagtatccga aatcggaggc cggcacccgc
     1501 gctcgccagc ggctcggcgg tcgctgacgt aaccatgaaa acgttcgcgg cgcgcgtgtc
     1561 cgagtggcag cgcgttcacg gacggcacga cctgccatgg cagcaggacc gcgatcccta
     1621 ccggatctgg ctgtccgaga tcatgctgca gcagacgcag gttgtgacgg tgctgccgta
     1681 ctacgcccgt ttcgtcgcag cgtttcccac tgtcgcaagt cttgccgcag cgccgctcga
     1741 tcacgtgctg gcggtttgga gtggactcgg ctattaccgg cgcgcgcatc atctgcatgc
     1801 tgccgccaat gcggtcgtac gcgatcaagg tggcgtgttt ccgcgtgatg cggcaacgct
     1861 tgcagcgctt ccgggcatcg gccgctcgac cgctgcagcg atcgctgcgt ttgcttcgga
     1921 cgagcgcggt gcgatcctcg atggcaacgt gaagcgcgtg cttgcgcgcc atcgcggcgt
     1981 cgacggatgg cccggcgagc cgcgcgtgca ggcggcgcta tggcaaattg ccgatgcgct
     2041 cgtgccgcca aacgcaagcg acattcgcgc gtacacgcag gggttgatgg acatcggcgc
     2101 gacgatctgc acgcgcacgc gacagcagtg tgctgtctgc ccgattcgcg aggactgcga
     2161 agcacgcctc actggccgag tcgccgagtt gccggcgccg cggccgcgca aggtgcttcc
     2221 acagcgcgag gtgacggtgc tgatgctcga gcgcgatggc gacgtgctgc tcgagcggcg
     2281 accggtactt ggtgtctggg gcggattgtg gagcttgccc gagcttccgc gtgacgctga
     2341 cattgcttcc catgtcggca tcaagctcgc gatgatgcct ggcgaattgc gccgactgcc
     2401 gccgctgacg catacgttca cgcacttcgc gctgacgatg tatccggttc gcgtgcaggt
     2461 cgcgcgtgcg ccactcgctg ttcggatgcc tggcgtcgaa tggttcgcac gcgatgcagc
     2521 gctcgcggcg gctattcctg cgccgattcg caagctgctg cagatttcgg aggctgagtt
     2581 tcgcttgacc ggttgacgat cgcgcgccgt cagcgtgctt cgatcaacgt accgtttttg
     2641 cccattcgcg cagatcctcc ggaagccgcc tgtccgcgtc gttgaacgcg gcgcggaaag
     2701 ctttgagtgc gcccgcggct tccgccatcc gtccatcgct gcgcaatgtt cgtatctgcg
     2761 cgatccattc gtcggggctc gaggttggcg cagcttgttt tgcgcgctgg tcggtgagcg
     2821 cgccaacggg tcgtgatgcc actgcggctg cgctcgcatc ggctgcggct gccggtgccg
     2881 aagacagtga cggtgcaggt gcaggcgctg cggcaggtgc cggcaccatc gagcgctccg
     2941 cgccggtcgt gttcgcttct gctggccgcg ataccgct
//
LOCUS       DASSVX010000355         6129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_349131, whole genome shotgun sequence.
ACCESSION   DASSVX010000355 DASSVX010000000
VERSION     DASSVX010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349131"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..998)
                     /locus_tag="VFO53_15070"
     CDS             complement(192..998)
                     /locus_tag="VFO53_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8877649.1"
                     /translation="MYTIDETFEIAVDTHSIAATLIIPDSRMPGVLFLHGWGGSQAQS
                     ATRAREIAALGCACLTVDMRGHAKTATQQATVTREDNLNDVLAAYDRLVREAAIDDDQ
                     IAVVGSSYGGYLATILTTLRPVKWLGLRAPALYKDTDWTSPKLALRQLQGLEAYRRMM
                     IEAQDNRALRAATAYRGDVLIVESGHDTIIPHEVIENYRNAFAGARSLTYRMIDGADH
                     GLTDPSCRQAYTTLLVNWITEMVAGKRAGDAASQKRLQEVMKTQKSAAAD"
     gene            complement(985..2133)
                     /locus_tag="VFO53_15075"
     CDS             complement(985..2133)
                     /locus_tag="VFO53_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3182 family protein"
                     /protein_id="HET8877650.1"
                     /translation="MMHEIESIGARGEGVAAKGDVVVYTAESCRAEHDHATCMAIART
                     LAAIKDYTFAGEYDVDGHYAKPLYFVPTDTLVGVGHACALGIAGEHDLFGGVVPHPFV
                     GTKCISHPLVDDRARVPDGWSEAFARRVRCAVLEGFAAFSTDDAFRAGLRLFAHGPVR
                     VKRALGIGGYGQTVVTGHEALERALADADTAEVARYGIALEQDLADVITHSVGQVRLG
                     ALLATYCGTQRLTPNNHGHPVYGGSDLLVARGGFDELLALQPAPSARRAVEQARVYDD
                     AAFACFDGLFASRRNYDIAEGNDAAGEHRCGVLEQSWRIGGASGAEVAALSAFRADPS
                     LAVVRASSVEVYGADASPPRDATVYFHGVDAHAGPLTKYFVLSRHVHD"
     gene            2409..4292
                     /locus_tag="VFO53_15080"
     CDS             2409..4292
                     /locus_tag="VFO53_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET8877651.1"
                     /translation="MQAWASAAWFGALLLAAGHAESQSLRDLSDLSLEELSQVEITSV
                     SKRPEPLSQAPAAVYVISSDDIRRSGATTLPEALRLAPNLEVARVDAQTYNISSRGMN
                     SVNASNKLLVLIDGRSIYTPFFSSVFWDQQSVMLADVERIEVISGPGGTLWGSNAMNG
                     VVNVITKSSADTQGGLVDAKLGDFVQRGAGRWGGRLGDLGTYRGYALGFGQGHTDLAT
                     GGSAMDDWRGTQAGFRADMSALASGFTVQGDIYNNLVDTPGGRRSGGNLLGRWNRRLG
                     DASSLSVQTFYDQQDRVVPAATGGSAERLRTFDIEAEHVFAIGSNQRFVWGAGQRNWQ
                     DRFVNTANPFVLQPESQTLSLTNLYGQDTIGFSEALKVTIGSKFEYSSLSGWAVMPNV
                     RAGFVADAKNFLWAAISRAVRSPSRLERDLTAPGIVNPSPDFQSEKLIAYEAGWRSQP
                     LSRATISVSLFYNDYTDLRTTKPAPVTILPVTFGNGWEGHTYGVDAWASYSPLAWWRL
                     DAGVELLRKSFHLKPGEIDIAGMQTVLGHDPGHQIFVHSYMDLTHDVELFVGLRQVGS
                     LPDVGVPSYFEADVTLGWRVTPRLELSLAGQNLVHARHAEAIAPPTFEIPRSVYLGAR
                     WSF"
     gene            4280..4867
                     /locus_tag="VFO53_15085"
     CDS             4280..4867
                     /locus_tag="VFO53_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HET8877652.1"
                     /translation="MEFLNLRGNLWWSRAGRLLPFAAAAWLAALAPAVGRAQSPSVEF
                     AVKATYLYKFAPFVEWPAGTFRSSADPLVLCVAGSDPVTDLVADAVKGQVVGGRAIDV
                     VRLLPAAHDPRCHILYVALKGAAAFAVLEKLRGTPVLTVTDAASDARATGIVNFVVQD
                     NRVRFEIDQRAAAENHLVISSKLLSLASRVSPRPG"
     gene            4885..>6129
                     /locus_tag="VFO53_15090"
     CDS             4885..>6129
                     /locus_tag="VFO53_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HET8877653.1"
                     /translation="MIDLRTILGMDRANFMPAVAAAGILVLVLAGAVMVMYNEQGYKE
                     AKTQQVEAQSRILATTVTAALAFDDRKAAQEYVKALAVDPQILVAAVYDARGLLLAGY
                     ARTPGVPVPASVHAARPTIEEHELFVTAPVAQGTTALGTVYLQAAIDPISRRFERYAV
                     LALLVTMASLIFVLLGVAQRRLTEVNEQLRRQGLDLGDANEKLRAQIEQRENAEAALR
                     QVQKMEAMGQLTGGIAHDFNNLLQVIIGNLEVLQRRNLVANDDGRRMVASAVRGAERA
                     STLTQRLLAFARRQPLDPKPVDVNKLVTGMSDLLHRTLGETVRIETVLTGGIWRVAVD
                     ANQLENALLNLAVNARDAMEGGGKLTIETANALLDEAYARANEIKPGQYVLIAVTDTG
                     AGMPNDVLAKAFEPFFTTKDVGK"
BASE COUNT         1016 a         2020 c         2056 g         1037 t
ORIGIN      
        1 agaggccgtt gcggcctcga tctaaaggcc ggatgtatgg ctgcaatcac tacctgctcg
       61 ctatcgcaaa tcggatgctt ggcaaaaagg gggcgaccat gtatgggttc ccgcttccgc
      121 gggaacgacg gtgctttgca aattgtcatg gagttcgagg cctgccatca ggcgaatgtg
      181 gcgcccgatc gtcaatcggc cgccgcgctt ttctgcgtct tcatcacttc ctggaggcgc
      241 ttctgcgatg cggcatcgcc cgcgcgcttg cccgccacca tctcggtgat ccagttcacg
      301 agcagcgtcg tatacgcctg ccggcacgag gggtcggtaa gtccgtgatc cgcaccgtcg
      361 atcatccggt aggtgagcga gcgcgcgccg gcgaacgcgt tccggtagtt ctcgatgacc
      421 tcgtgcggaa tgatcgtgtc gtgcccgctt tcgacgatca gcacgtcgcc gcgatacgcg
      481 gtggcggcac gcagcgcgcg gttgtcctgc gcctcgatca tcatccggcg atatgcctcg
      541 agcccctgca actggcgcag cgcgagcttc ggcgacgtcc agtcggtgtc cttgtacagc
      601 gccggcgcac gcagcccgag ccacttcacc ggccgcagcg tggtgaggat cgtcgcgaga
      661 tagccgccgt agctgctgcc gacgaccgcg atctggtcgt cgtcgattgc cgcctcgcgc
      721 accagccggt cgtacgcggc gagcacgtcg ttgaggttgt cctcgcgcgt caccgtcgcc
      781 tgctgcgtcg ccgtcttcgc gtgtccgcgc atgtcgacgg tgaggcaggc gcagccgagc
      841 gccgcgattt cacgcgcacg cgtcgccgat tgggcctggc ttcctcccca gccgtgcagg
      901 aacagcacgc cgggcatccg cgaatcggga atgatcagcg tcgcggcgat gctgtgcgtg
      961 tcgaccgcga tctcgaacgt ttcgtcaatc gtgtacatgg cgcgaaagca cgaagtactt
     1021 ggtcaacgga ccggcatgcg cgtcgacgcc gtgaaaatac acggtggcgt cgcgaggcgg
     1081 cgatgcatcg gcgccgtaca cctcgacgga cgatgcgcgg accacggcga gcgaaggatc
     1141 ggcgcgaaat gcggacagcg ccgccacctc ggcaccgctg gccccgccga tgcgccacga
     1201 ttgctcgagg acgccgcagc ggtgctcgcc ggccgcatcg ttgccctccg cgatgtcgta
     1261 attgcgccgc gacgcaaaca gtccgtcgaa gcatgcgaac gcagcgtcgt cgtacacgcg
     1321 cgcctgctcg acggcgcgac gtgccgaggg cgcgggttgc agcgcgagca gttcgtcgaa
     1381 tccgccgcgc gcgacgagca gatcggatcc cccgtagacc gggtgtccgt ggttgttcgg
     1441 cgtgagacgc tgcgtgccgc aatacgttgc gagcagggcg ccgaggcgca cctgtccgac
     1501 gctgtgcgtg atgacatcgg caaggtcctg ctcgagcgcg atgccgtagc gcgccacttc
     1561 ggcggtgtcg gcatccgcaa gcgcacgttc gagcgcctcg tgaccggtga cgaccgtctg
     1621 cccgtagccg ccgatgccaa gcgcgcgctt cacacgcacc ggtccatgcg caaacagccg
     1681 cagccccgcg cggaatgcgt cgtcggtgga gaacgccgcg aatccttcga gcaccgcgca
     1741 acgcacgcgg cgcgcaaacg cttcggacca gccgtcggga acgcgcgcgc gatcgtcgac
     1801 gagcggatgg ctgatgcatt tggtgccgac gaacgggtgc ggaacgacgc cgccgaagag
     1861 gtcgtgctcg ccggcgatgc cgagtgcgca cgcatgcccc acgccaacga gcgtgtcggt
     1921 cggcacgaaa tagaggggct tcgcgtagtg accatcgacg tcgtattcgc cggcgaacgt
     1981 gtaatccttg atggcggcga gcgtccgcgc gatggccatg caggtggcgt ggtcgtgctc
     2041 ggctcggcag ctctccgcgg tgtacaccac gacatcgccc ttcgctgcga cgccctcgcc
     2101 gcgagcgccg atcgactcga tttcgtgcat cattggacgc ctccccgatg ggcggtgcga
     2161 gcgcgaaccg tcgcctgccc ctgttcgcag cgcgcttcga ttagaccttg ccgtgccgga
     2221 ttcattgcgc cgcggcgtcc acgtaggcgg aactccgacg tttcgcttcg ccggccgcag
     2281 gctttcgtgt attttttcgc cggtggcatg accgaaaatc cgaaaaaaag gagccgatct
     2341 tgaccttgcg aacccgaccg ggaggcggca cccccgcatc gcgaagcacg ccatacgggt
     2401 ggcggcgttt gcaagcgtgg gcgagcgccg cgtggttcgg cgcactgctc ctcgcggccg
     2461 gccatgccga gtcgcaatcg ctgcgcgacc tgtccgacct gtcgctcgag gagctttcgc
     2521 aggtcgagat cacgtcggtg tcgaagcggc ccgagccgtt gagccaggcg ccggcggcgg
     2581 tctacgtgat cagcagcgac gacattcgcc gctcgggcgc taccacgctg ccggaagcgc
     2641 tgcgcctcgc gccgaatctc gaggtcgcgc gcgtcgatgc gcagacgtac aacatctcgt
     2701 cgcggggcat gaattcggtc aatgcgtcga acaagctcct cgtgctgatc gacggacgca
     2761 gcatctacac gccgttcttc tcgagcgtgt tctgggatca gcagagcgtc atgcttgccg
     2821 acgtggagcg catcgaggtc atcagcgggc cggggggcac gctgtggggc tcgaacgcga
     2881 tgaacggcgt ggtgaacgtc atcaccaaaa gctcggccga cacgcagggc gggctcgtcg
     2941 acgcgaagct cggcgatttc gtgcagcgcg gcgcgggccg ctggggcggg cgtctcggcg
     3001 atctcggcac ctaccgcggt tacgcgctcg gcttcgggca gggccatacc gacctcgcaa
     3061 ccggtggcag cgcgatggac gactggcgcg gcacgcaggc gggctttcgc gccgatatgt
     3121 cggcgctcgc gagcgggttc accgtgcagg gcgacatcta caacaatctc gtcgacacgc
     3181 cgggcggacg gcgcagcggc ggcaacctgc tcggacgctg gaacaggcgc ctcggcgacg
     3241 cttcttcgct ttcggtgcag acattctacg atcagcagga ccgcgtcgtg cccgcagcga
     3301 cgggaggcag cgccgaacgg ttgcgcacgt tcgacatcga ggccgagcac gtgttcgcga
     3361 tcggcagcaa ccagcggttc gtctggggcg ccggacagcg gaattggcag gaccgcttcg
     3421 tcaataccgc gaacccgttc gtgctgcagc ccgaaagcca gacgctcagc ctcaccaacc
     3481 tctacggtca ggacacgatc ggcttctccg aagcgctcaa ggtgacgatc gggtccaagt
     3541 tcgagtacag cagcctgagt gggtgggcgg tgatgcccaa cgtgcgcgcg ggcttcgtgg
     3601 ccgatgcgaa aaatttcctg tgggccgcga tctcgcgcgc cgtgcgctcg ccgtcgcgtc
     3661 tcgagcgtga tctcacggcg ccgggcatcg tcaacccatc gcccgatttc cagtcggaga
     3721 agctgatcgc gtacgaggcg gggtggcgct cgcaaccgct gtcgcgcgcg acgatctcgg
     3781 tgtcgctgtt ctacaacgac tataccgacc tgcgaacgac gaaaccggcg ccggtcacca
     3841 ttcttcccgt cacgttcggc aacggttggg aagggcacac gtacggcgtc gatgcatggg
     3901 ccagctacag cccgctcgca tggtggcgcc tcgatgcggg cgtcgagttg ctgcgcaagt
     3961 cgtttcatct caagccgggt gaaatcgaca ttgccgggat gcaaacggtg ctgggtcacg
     4021 atccgggtca tcagatcttc gtgcactcgt acatggatct cacgcacgat gtcgagcttt
     4081 tcgtcgggct gcggcaggtg ggcagccttc cggacgtcgg cgtcccgagc tacttcgagg
     4141 ccgacgtcac gctgggctgg cgtgtgacgc ccaggctcga gctgtcgctt gccgggcaga
     4201 atctggtgca tgcgcgacac gcggaagcca tcgcaccgcc gaccttcgag attccgcgaa
     4261 gcgtctatct cggggcgcgg tggagtttct gaacctacgc gggaacctgt ggtggtcccg
     4321 cgcgggccgc ctgctgccgt tcgcggccgc agcctggctc gcggcgctcg cgccggccgt
     4381 tggccgcgcc cagtcgccga gcgtcgagtt cgcggtgaag gcaacctatc tttacaagtt
     4441 cgcgccgttc gtcgagtggc ccgcgggcac ctttcgatcg agcgccgatc cgctcgtcct
     4501 ctgcgttgcc ggcagcgatc cggtgaccga tctcgttgcc gatgcggtaa aaggtcaggt
     4561 cgttggcggc cgtgcgatcg acgtcgttcg cctgctgccg gcggcgcatg atccgcgatg
     4621 tcatatcctc tacgttgcgc tgaagggcgc agcggccttt gccgtactcg aaaagctgcg
     4681 cggcacaccg gtgctcacgg tgaccgacgc ggcgagcgac gcgcgcgcaa cgggaatcgt
     4741 gaacttcgtc gtgcaggaca atcgcgtgcg cttcgaaatc gaccagcgcg ccgcggcgga
     4801 aaatcacctc gtcatcagct cgaagcttct gagccttgcg agccgcgtgt cgcccaggcc
     4861 tggctagatg tattcgtgca aaaaatgatc gacttgcgca cgatcctcgg catggaccgc
     4921 gcgaacttca tgccggccgt tgctgcggcg ggcattctcg tgctcgtgct cgccggcgcc
     4981 gtgatggtca tgtacaacga gcaggggtac aaggaggcga agacgcagca ggtggaggcg
     5041 cagagccgca tcctcgcgac cacggtcacc gccgcgctcg ccttcgacga ccgcaaggcg
     5101 gcacaggaat atgtgaaggc gctcgccgtc gatccacaga ttctcgtcgc cgccgtgtac
     5161 gacgcgcgcg gactgctcct cgccggctac gcgcgcacgc ctggcgtgcc ggtccctgcc
     5221 tccgtccatg ccgcgcggcc gaccatcgaa gaacacgagc tcttcgtcac cgcacccgtc
     5281 gcacagggga cgacggcgct cggcaccgtc tacctgcagg ccgcaatcga tccgatttca
     5341 cgccgcttcg agcgttacgc ggtgctggcg cttctcgtga cgatggcgtc gctgatcttc
     5401 gtgctgctcg gcgtcgcgca acggcggctc accgaggtca acgagcaatt gcgccgccag
     5461 ggactcgatc tcggcgacgc caacgagaag ctgcgcgcac agatcgagca gcgcgagaac
     5521 gccgaggcgg cgctgcgcca ggtgcagaag atggaggcga tgggacagct cacgggtggc
     5581 atcgcgcacg acttcaacaa cctgctgcag gtcatcatcg gcaatctcga agtgctgcaa
     5641 cggcgcaatc tcgtcgcgaa cgacgatgga cggcggatgg tggcgtcggc cgtgcgcggt
     5701 gccgaacgcg catcgacgct gacgcagcgg ctgctcgcat ttgctcgacg ccagccgctc
     5761 gatccgaagc cggtcgacgt caacaagctc gtcaccggca tgtcggatct gctgcaccga
     5821 acgctcggcg aaacggtccg gatcgaaacc gtgctgaccg gcggcatctg gcgggttgcg
     5881 gtcgacgcga accagctcga gaacgcgctg ctcaacctcg ccgtcaacgc gcgcgacgcg
     5941 atggagggcg gcggcaagct gacgatcgag acggccaatg cgctcctcga cgaggcgtac
     6001 gcgcgcgcaa acgagatcaa gccgggacag tacgtgctga tcgcggtgac cgacacgggc
     6061 gcgggcatgc ctaatgacgt tctcgcgaag gcgttcgagc cgttcttcac cacgaaggac
     6121 gtcggcaag
//
LOCUS       DASSVX010000356         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_199117, whole genome shotgun sequence.
ACCESSION   DASSVX010000356 DASSVX010000000
VERSION     DASSVX010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199117"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VFO53_15095"
     CDS             <1..826
                     /locus_tag="VFO53_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HET8877654.1"
                     /translation="EITAAVPPGRHVGRRGEDIRAGAAVLEPGRRLRPQDAGLVASLG
                     IADIDVVASPRVRIVVTGNEIVSAGTEKTSFQIYDANSPMLRALVVRDGGVVESHRRI
                     GDEVARIRDALTAPGADIILVSGGSSVGSEDHAPMLLKEVGELAIHGVAMRPSSPAGM
                     GRTRDALVFLLPGNPVSCLCAYDFFAGRAIRIRGGRPAAWPHRQRPGKLARKIASAIG
                     RVDYCRVRMSGDAVEPLALSGASILSSTTRADGFVIVPSESEGFGADTEVNVYLYD"
     gene            819..>2701
                     /locus_tag="VFO53_15100"
     CDS             819..>2701
                     /locus_tag="VFO53_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HET8877655.1"
                     /translation="MTDRDTHDATQHVAPPDARQTQFLTVITRDEATARFQRHLRLAP
                     LAVESVPLSRALHRVLADDIAAYVDVPGFDRSNVDGFAVQAADTHDAAEEAPRTLALD
                     AEVLAPGVTPRHAVASGRATMIATGGMVPRGADAVAMVEHTEPVDDGRRLEVSRPLAP
                     GDNVSFAGTDIAKGETVLRAGQLLTSREIGVLAAIGRADVSVYCRPRVAIISTGNELV
                     APGAPLPIGAIYDSNAATIAAAVEELGCTAVQLGIVADTDDAVASAVQDALAYDVVVL
                     SGGTSKGAGDLSYRVVSRLGNPGIVAHGVALKPGKPICLAVTNGKPVVILPGFPTSAM
                     FTFHEFVAPVLRAYAGLPPDRRQIVPATLAVRVNSERGRTEYLLVGLVPVADGYAAYP
                     MGKGSGSVTAFSNADGFITIDQHTEIVDAGATVSVQLLGQRLEPADLVIIGSHCVGLD
                     VIVGELTRRGVRTKVLNVGSMGGLAAARRGECDIAGIHLMDPKTGEYNRHLLTDALTL
                     EPGYGRMQGIVHREGDPRFRRKDAQAALAAVLGDPDCTMVNRNTGSGTRILIDRLLGN
                     HRPPGYAVQTKSHNAVAAAVTQSRADWGIAIDTVAHAYDLRFIPLQAEQYDFVIPKAR
                     ADRA"
BASE COUNT          430 a          909 c          896 g          466 t
ORIGIN      
        1 ggagatcacc gcggcggtgc cgccgggacg ccacgtcggc cgccgtggcg aggacattcg
       61 cgcaggggcc gcagtgctcg agcccggccg tcgcctgcgg ccgcaggacg ccggcctcgt
      121 cgcctcgctg ggcatcgccg acatcgatgt cgtggcgtca ccgcgagtgc gaatcgtcgt
      181 caccggtaac gagatcgtca gcgccggcac ggagaaaaca tccttccaga tttacgacgc
      241 gaattcccct atgctgcgcg cactcgtcgt tcgcgacggc ggtgtcgtcg aatcgcaccg
      301 gcgaattggc gacgaggtcg ctcggattcg tgacgcgctg acggcaccgg gtgccgacat
      361 cattctcgtt tccggcggtt cgagtgtggg cagcgaagac catgcgccga tgctgctgaa
      421 ggaagtcggc gagctcgcca tccacggcgt cgcgatgcgc ccatcgagcc ccgcgggtat
      481 gggtcgtacg cgcgatgcgc tcgtgttcct gctgccgggc aatccggtct cgtgtctctg
      541 cgcctacgat ttctttgccg gccgcgcgat tcgcatccgc ggcggacgtc ccgcagcgtg
      601 gccgcaccgg cagcgaccgg gaaaactcgc gcgcaagatc gcctccgcga tcgggcgtgt
      661 cgactattgc cgcgtgcgaa tgtccggcga cgccgtcgag cccctcgcgc tttccggcgc
      721 gtcgatcctg tcttcgacga cgcgcgccga tggcttcgtc atcgttcctt cggagtctga
      781 agggttcggc gcggacaccg aagtgaacgt ctacctctat gactgatcgc gacacgcacg
      841 acgcaacgca gcacgttgca ccacccgatg cgcggcagac gcagttcctg acggtgatca
      901 cgcgcgatga agcaaccgcg cgctttcagc ggcatctgcg gcttgcgccg cttgcagtcg
      961 aatcggtgcc gctcagccgc gcgctgcatc gcgtgctggc cgacgacatc gccgcctatg
     1021 tcgacgtgcc cggcttcgac cgctcgaatg tcgatggctt cgccgtgcag gccgccgaca
     1081 cgcacgacgc cgcggaggaa gcacctcgaa cgcttgcgct cgatgcggaa gtgctcgcac
     1141 cgggtgtgac gccgcgccat gccgtcgcga gcggacgcgc gacgatgatc gcaacgggcg
     1201 gcatggtgcc gcgcggcgcc gacgccgttg cgatggtcga gcacaccgag cccgtcgacg
     1261 acggtcgacg gctggaagtg agccggccgc tcgcacccgg cgacaacgtg agctttgccg
     1321 gcaccgatat cgcgaaaggg gagacggtgc ttcgcgccgg gcagcttctg acgtcgcgtg
     1381 agattggcgt gctcgctgca atcggtcgcg ccgacgtgtc ggtttattgc cgtccgcgcg
     1441 tcgccatcat ctcaacgggc aacgagctcg tcgcacccgg tgcgccattg ccgatcggcg
     1501 cgatctacga ctcgaacgca gcgacgatcg ctgccgccgt ggaagagctc ggatgcacgg
     1561 ccgtgcagct tggcatcgtc gccgacaccg acgacgccgt tgcttccgcc gtgcaggatg
     1621 cgcttgcgta cgacgtcgtc gtgctgtcgg gcggcacgtc gaaaggtgcg ggcgatttgt
     1681 cttatcgcgt cgtcagcagg ctcggcaatc cgggcatcgt tgcgcacggc gttgcgctga
     1741 agcccggcaa gccgatctgc ctcgcggtga ccaacgggaa gcccgtcgtc atcctgccgg
     1801 gattcccgac gtccgcgatg ttcacgttcc acgaattcgt tgcgccggtg cttcgcgctt
     1861 atgcgggtct gccgccggat cggcgccaga tcgtgccggc gacgctggcc gttcgcgtca
     1921 actccgaacg cgggcgtacc gagtatctgc tcgtcggact cgttcctgtt gccgacggtt
     1981 acgccgcgta tccaatgggc aaaggctcgg gctcggtcac ggcgttcagc aatgccgatg
     2041 gcttcatcac catcgatcag cacaccgaga tcgtcgacgc gggcgcaacc gtgtccgtgc
     2101 agttgctggg ccagcgactc gagcccgccg atctcgtcat catcggcagt cattgcgtag
     2161 ggctcgacgt gatcgtgggc gagttgacgc gccgcggcgt gcgaacgaaa gtgctgaacg
     2221 tcggcagcat gggcggcctc gcggcggcgc ggcgaggcga atgcgacatc gccggcatcc
     2281 acctgatgga tccgaaaacc ggcgaataca accgtcatct gctgacggat gcgttgacgc
     2341 tcgagccggg ttacggccgc atgcagggga tcgttcatcg cgaaggcgat ccacgctttc
     2401 gccgcaagga tgcgcaggcg gcacttgcgg ccgtgctcgg cgatcccgat tgcacgatgg
     2461 tgaatcgcaa taccggcagc ggcacgcgaa tcctgatcga ccggctgctc ggaaaccacc
     2521 gacccccggg ctacgccgtg cagacgaaat cccacaatgc cgtcgccgca gcagtgacgc
     2581 agtctcgagc cgattggggt atcgccatcg ataccgtcgc gcatgcctac gacctgcgct
     2641 tcatcccgct gcaggccgag caatacgact tcgtcattcc gaaggcgcgc gctgaccgcg
     2701 c
//
LOCUS       DASSVX010000357         7577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_532335, whole genome shotgun sequence.
ACCESSION   DASSVX010000357 DASSVX010000000
VERSION     DASSVX010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532335"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..902
                     /locus_tag="VFO53_15105"
     CDS             207..902
                     /locus_tag="VFO53_15105"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8877656.1"
                     /translation="MGRTPARLAAADAPLADIEQRLRNDIVSGDLPFGARLRIEDLAL
                     RYGVSHMPVREALRVLHGEGLVVIEPNRGARVRPIYRGFIEDVFDMRCAIETMLARRA
                     AERRTEAHLARLRCAAERLEACVESGDFPGVPHANREFHATINDAAGNPGALPIVDSH
                     WLLLSALLKRYGYGDERFPRVIDEHQHLIQAIERHDAHSAALLMGAHIEKAKNNLVRR
                     AADALPEEPARAL"
     gene            943..1692
                     /locus_tag="VFO53_15110"
     CDS             943..1692
                     /locus_tag="VFO53_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8877657.1"
                     /translation="MLVGVASLALVIGVYWYMALRPGTNPALVPSPPAILKAFRDEIA
                     SGDLLVNALASLRRVVIGFLIGASLALVLGALAGWFRYAGYVLNPIIDALRPIPALAY
                     IPLVIVWVGIGEPSRIIIIVLAVFKPCVVNARAGMKEVAQIYVDAARTLGASRWRVFT
                     TVALPSAVPYFIAGLRTGVSTGFLALVAAELIAAPSGLGFMIQNAGQYFRTDVTIVGI
                     IVIGLLGALLDQLASRTGRALTAWSDVRREP"
     gene            1815..2627
                     /locus_tag="VFO53_15115"
     CDS             1815..2627
                     /locus_tag="VFO53_15115"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8877658.1"
                     /translation="MRGLTHRYVMGAPPSELTVLSDIDLVIARGEFVCIIGESGCGKS
                     TLLRMMAGLLTPSEGDVLHDGAKVAGVRHELGFVFQQDAVFPWLTVEKNVEYGPKSRG
                     VRRAERERLVAQWCEAVGLTAFRKAYPKQLSGGMRKRVDLARAYANSPDVLLMDEPFA
                     ALDVQTKAEMQDAVLELWQSTGKTVVFVTHDLDEAVFLSDVVVVLSSRPGRVHSIVHS
                     TLPRPRGAETRVSDGFAAAKRTLWTTLQEARGRPIAVVNEHTSPGRKHHVEP"
     gene            2614..3678
                     /locus_tag="VFO53_15120"
     CDS             2614..3678
                     /locus_tag="VFO53_15120"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877659.1"
                     /translation="MSNRDAASGRRRFLVRAGAGAMLATAGSLGFGALARAQPAGGAP
                     LSIGLLRAPASGIIDLTEQHGWFRQAGVTLNEVLFVAAAGPKIIQALGSGGIGLSFVN
                     STAALLGLAGGVIPLRFISIPTDPSRLFALLAAPGIESVPKLAGKRVAATGGTALHYF
                     LARVLAKFDMTLKDIEFVNLAAADGQAAFVAGRVDAIVPSVNGRFYIMSTRKETRELF
                     TYDDFTKPPGPTKAFLNYDLFVTTQSVLASSAPALKGFLAAYHDKGVRYLLDPKTRPQ
                     AIAGITEYVNKEQKNPTDAAIMSRIIELSGFYDLDTVKGLMTRDDFRASLEYQVKFFM
                     DLGQIKTAPDLGKAIVTNLL"
     gene            complement(3692..4399)
                     /locus_tag="VFO53_15125"
     CDS             complement(3692..4399)
                     /locus_tag="VFO53_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8877660.1"
                     /translation="MPRQPRFYYSGAVLHVVQRGNNRAPVFTSAADHRFYLDCLRDAS
                     RTHEVTIHAYVLMTNHVHILASPAHERALPRMMQTVGRRYVGRFNFLQRRTGTLWEGR
                     YKATLVDREAYLFSCLRYIELNPVRACMVPTPGDYRWSSHRANAFGDDDPVITPHPSF
                     LALGSTNEERRDTYLRMFGEPLHAETVRAIRDATQFEWALGDAAFREVVETRTGRRAH
                     RLPIGRPVSHDAGKSRL"
     gene            complement(4841..5173)
                     /locus_tag="VFO53_15130"
     CDS             complement(4841..5173)
                     /locus_tag="VFO53_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877661.1"
                     /translation="MSTLAIDTYKLLTALKGTGKSANFTAEEIANAVDVAQERAELLT
                     RPVFEARMATFEARMNGFDAKLEVIRSDIKAEVKASQLQNVFWLSGIMLASNGIVIAL
                     LARLAHVI"
     gene            complement(5223..>7577)
                     /locus_tag="VFO53_15135"
     CDS             complement(5223..>7577)
                     /locus_tag="VFO53_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET8877662.1"
                     /translation="PEFGAASQLEKIDMLDFANFVAINKFDRKGAQDALRDVRKQYQR
                     NRTLFNVAPDEMPVYGTIASRFNDDGVTALYQAIAERLQTHGLAVKKGALASLTTRCS
                     TQRQAVVPASRVRYLAEIAETVRGYHEWAGRQSNIARERQQLRAAKAMLGTKVGSETT
                     FGNASLNVSEPAAPKVVSDPTLVALDALIAGREAGLDPRSKKLLDMWPRTKAAYAGDE
                     YIVKIRDREIRTALTTTSLSGTKVPKVVLPKYEDDGEILRWQLTENVPGSFPFTAGVF
                     AFKRENEDPTRMFAGEGDAFRTNKRFHLLADGMPAKRLSTAFDSVTLYGNDPDPRPDI
                     YGKVGNSGVSIATLDDMKVLYAGFDLCAANNSVSMTINGPAPTILAMFMNTAIDQQLD
                     KFSGDNGREPTDDEVAKIRAWTLATVRGTVQADILKEDQGQNTCIFSTEFSLKVMGDI
                     QQYFVHHNVKNFYSVSISGYHIAEAGANPISQLAFTLANGFTFVEAYLARGMHIDDFA
                     PNLSFFFSYGMDPEYAVIGRVARRIWAVAMRERYGANERSQKLKFHSQTSGRSLHAQE
                     IAFNDIRTTLQALISTYDNTNSLHTNAYDEAITTPTEESVRRAMAIQMIINREWGLGN
                     NENPNQGSFIVEELTMLVEEAVLKEFEAISERGGVLGAMETGYQRGKIQEESLDYEHR
                     KHDGSYPIVGVNTFRNPHGDAVVQKLELARSTDDEKQSQLARLAEFHARHAHEAPAAL
                     ARLQQTVIDDGNVFEELMRTVRVCSLGQITNALFDVGGQYRRSM"
BASE COUNT         1209 a         2502 c         2490 g         1376 t
ORIGIN      
        1 atgcaccagg gcgaagcggc attcgcactg ctcggccagg cgctcgacga cggctacggc
       61 gccgatgaca cccccggctc gatgctgcgc gcatgcgaag cgcgcgccgg cgtgcatctc
      121 gtcccctgaa atgagccccc cacgctcgct gcgctcgctg cccccccccg gggggcgatc
      181 agtgcttggg gcggcccttc gcactgatgg gtcgtacgcc cgcgcgtctc gcggcggccg
      241 atgcgccgct cgccgacatc gagcagcggc tgcgcaacga catcgtgtcg ggcgacctgc
      301 cgttcggcgc acgccttcgc atcgaggatc tcgcactgcg ctatggcgtg agccatatgc
      361 cggtgcgtga agcactgcgc gtgctccacg gcgagggcct cgtcgtcatc gagccgaatc
      421 gcggcgcacg cgtgcggccg atctatcgcg gcttcatcga ggacgtgttc gacatgcgct
      481 gtgcgatcga gacgatgctc gcgcgccgcg cagccgagcg caggacggag gcgcacctgg
      541 cgcgattgcg ttgcgccgcg gagcggctcg aggcgtgcgt cgaaagcggg gattttccag
      601 gggttccgca tgccaatcgc gaattccacg cgacgatcaa cgacgcggcc ggcaatcccg
      661 gcgcgctccc gatcgtcgac agccactggc tgctcctgtc agcccttctc aagcgttacg
      721 gctatggcga cgagcgcttt ccgcgcgtga tcgacgagca tcagcacctg atccaggcga
      781 tcgagcgcca cgatgcgcat tcggcggcgc tcctgatggg tgcgcatatc gaaaaggcga
      841 agaacaacct ggtgcgccgc gcggccgacg cgctgccgga ggagccggcc cgtgcgctct
      901 agcgtgcgcc gttcgtggat tgcgcggttt tgcaagccgt ccgtgctcgt cggcgtggcg
      961 tcgctggcgc tcgtcatcgg tgtctactgg tacatggcgc tgcggcccgg cacgaacccg
     1021 gcgctggtac cctcgccgcc cgcgatcctg aaggcatttc gcgacgagat cgcaagcggc
     1081 gacctgctcg tcaacgcact cgcgagcctc cggcgcgtcg tgatcggctt tctcatcggc
     1141 gcgtcgctcg cgctcgtgct cggcgcgctc gccgggtggt ttcgctacgc aggctacgtg
     1201 ctgaacccga tcatcgacgc gctgcggccg attcccgcgc tcgcgtacat cccgctcgtg
     1261 atcgtctggg tcggcatcgg cgagccgtcg cggatcatca tcatcgtgct cgccgtgttc
     1321 aagccgtgcg tcgtcaacgc gcgcgccggc atgaaggagg ttgcgcagat ctacgtcgac
     1381 gcggcgcgca cgctcggcgc gtcgcgctgg cgcgtgttca cgacggtcgc gctgccgagc
     1441 gccgtgccgt atttcatcgc cggcctgcgt accggcgtat cgaccggctt tctcgcgctc
     1501 gtcgcggccg agctgatcgc cgcgccgtcg ggcctcggct tcatgatcca gaacgccggg
     1561 cagtacttcc gcaccgacgt gaccatcgtc ggcatcatcg tcatcggcct cctcggcgcc
     1621 ctgctcgacc agctcgcgag ccgcaccggc cgcgcgttga cggcgtggag cgacgtgcgc
     1681 cgcgagccat gaaacctcgt tggcacattg cgcccactcg cgccgcggag ccgtctcaag
     1741 gcgcgagttc cgcccccacc gggggcagcg cagcggcgaa gccgcaagcg tgggggggac
     1801 agcccaagct tgcggtgcgc ggcctcacgc atcggtacgt catgggcgcg ccgccgtccg
     1861 agctcaccgt gctgtcggac atcgacctcg tcatcgcgcg cggcgagttc gtctgcatca
     1921 tcggcgaatc gggctgcggc aaatcgacgc tgctgcgcat gatggcgggc ctgctcacgc
     1981 cgtccgaagg cgacgtgctg cacgacggcg caaaggtcgc aggcgtgcgt cacgagctcg
     2041 gcttcgtttt tcagcaggac gccgtctttc cgtggctcac cgtcgagaag aacgtcgaat
     2101 acggcccgaa gtcgcgcggc gtccgaaggg ccgaacgcga gcggctcgtc gcgcaatggt
     2161 gcgaagcggt cggattgacc gcgttccgga aggcgtatcc gaagcagctt tcgggcggca
     2221 tgcgcaagcg tgtcgatctt gcgcgcgcct acgcgaactc gcccgacgtg ctgctgatgg
     2281 acgagccgtt cgcggcactc gacgtgcaga cgaaggccga aatgcaggac gcggtgctcg
     2341 agctttggca gagcaccggc aagacggtcg tcttcgtaac gcacgacctc gacgaggcgg
     2401 tgttcctgtc cgatgtggtc gtcgtgctgt cgagccggcc gggacgcgtg cattcgatcg
     2461 tccacagcac gctgccgcgg ccacgcggcg ccgagacgcg cgtcagcgac ggcttcgccg
     2521 cggcgaaacg gacgctgtgg acgacgctgc aggaggcgcg cgggcgtccg atcgcggtag
     2581 tcaacgagca cacttcacca gggaggaaac accatgtcga accgtgacgc cgcatcgggc
     2641 cgccgtcgct ttctcgttcg cgccggtgcc ggcgcgatgc tcgcgacggc cggatcgctc
     2701 ggcttcggcg cgcttgcgcg cgcgcagccg gcgggcggtg cgccgctgtc gatcgggctc
     2761 ctgcgcgcgc ctgcgtcggg aatcatcgac ctcaccgagc agcacgggtg gttcaggcaa
     2821 gccggcgtca cgctgaacga ggtgcttttc gtcgcggcgg ccggaccgaa gatcatccag
     2881 gcactgggca gcggcggcat cggcttgtcg ttcgtcaatt cgacggcggc gctgctcgga
     2941 ctcgccggcg gggtgattcc gctgcgcttc atttcgattc cgaccgatcc gtcgcggctt
     3001 ttcgcgctgc tcgcggcccc gggtatcgaa agcgttccca agctcgccgg caagcgcgtc
     3061 gcggcgacgg gcggcacggc gctccactac ttcctcgcgc gcgtgctcgc caaattcgac
     3121 atgacgctga aggacatcga attcgtcaat ctcgccgccg cggatggcca ggcggcgttt
     3181 gtcgcgggac gcgtcgacgc gatcgtcccg tcggtcaacg gccgcttcta catcatgagc
     3241 acgcgcaagg agacgcgcga gctgttcacg tacgacgact tcacgaagcc gccggggccg
     3301 acgaaagcgt tcctgaacta cgacctgttc gtcacgacgc aaagcgtcct ggcgtcgagc
     3361 gctccggcgc tcaagggctt tctcgcggca taccacgaca agggcgttcg ctatctgctc
     3421 gacccgaaga cgcgcccgca ggcgattgcc ggcatcaccg aatacgtcaa caaggagcag
     3481 aagaatccga ccgacgcggc gatcatgagc cgcatcatcg agctgtcggg cttctacgac
     3541 ctggacacgg tgaagggtct gatgacgcgc gacgattttc gcgcgagcct cgagtaccag
     3601 gtgaagttct tcatggatct cgggcagatc aagacggcgc ccgacctggg caaggcgatc
     3661 gtgaccaatc tgctgtaata ccgaagtagg gtcagagacg acttttccct gcatcgtggc
     3721 ttacggggcg ccctatcggc agacgatggg cacgccgccc agtcctcgtc tcgaccacct
     3781 cgcgaaatgc cgcgtcgccg agtgcccact cgaactgcgt tgcatcgcga atggcccgaa
     3841 ccgtttccgc atgcaagggt tcgccgaaca tccggagata ggtatcgcgc cgttcctcgt
     3901 tcgtcgaccc caaggcgagg aacgacggat gcggcgtgat cacggggtcg tcgtcgccaa
     3961 aagcattggc gcggtggctt gaccaacgat agtcgcctgg tgtcggcacc atgcatgcgc
     4021 gcactggatt cagctcgatg taacgaaggc aggaaaagag gtacgcctcc ctgtcgacga
     4081 gcgtagcctt gtaccggcct tcccatagcg ttccggtacg ccgctgcagg aagttgaacc
     4141 tgccgacgta tctccgccca acggtctgca tcatgcgcgg cagtgcccgt tcatgggccg
     4201 gcgaggcgag gatgtgcacg tgattggtca tcagcacata cgcgtgaatc gtcacctcgt
     4261 gagtgcgtga tgcgtcgcgg aggcaatcga gatagaagcg atgatcggcg gcagaggtga
     4321 acaccggtgc gcggttgttg ccgcgctgaa cgacgtgcag cacggcgccg gaataataga
     4381 agcgcggttg acgtggcatg cgcagagcgt gcgctcacct ggcatgcggt cgccatcatc
     4441 cataagtagg gtcagagacg cctttcgtgc gctctcggct gcagctcgtt ccggcgatga
     4501 acgtcttctc cgaccttact tcggctcgca gcgagacgag atcacggcga accctagcgc
     4561 gccttccgca cggcgggccg aaacggatgc aaggctcgga aaacttcgcc ggctgcgtaa
     4621 agacgctgcg cgcattcagc ccatgacgaa gtcttcgctg ttatcgaacc cgaggatcgt
     4681 cgtcgatcct ggccatctat gtagtatggg gccatccgaa agtcgtctct gaccctacat
     4741 attccttcat ggatctcggg cagatcaaga cggcgcccga cctgggcaag gcgatcgtga
     4801 ccaatctcgt gtagtgctgg caaacgcgcc gcgttcgccg tcagatgacg tgggcgagcc
     4861 gcgcaagcag tgcgatgacg atgccgttgc tcgcgagcat gatgcccgaa agccaaaata
     4921 cattctgcaa ttggctggcc ttcacttcgg ccttgatgtc gctccggatc acttcgagct
     4981 tggcgtcgaa gccattcatt ctcgcctcga aggtcgccat ccgcgcctcg aagaccggcc
     5041 tcgtcagcaa ttcggcccgt tcctgcgcta cgtcgaccgc gttcgcgatc tcctcggcag
     5101 tgaagttcgc gctcttgccg gttcccttga gcgccgtgag gagcttgtag gtatcgatgg
     5161 cgagggtcga catccgtcgg aggctatcga cgagacgagt atagcgccat cggctgctcg
     5221 gcctacatgc tgcgccggta ctgcccgccc acgtcgaaga gcgcattcgt gatctggccg
     5281 agcgagcaga cgcgcaccgt gcgcatcagt tcctcgaaca cgttgccgtc gtcgatcacc
     5341 gtctgctgca accgcgcgag cgcggccggc gcctcgtgcg cgtgacgcgc atgaaattcg
     5401 gcgaggcgcg cgagctgcga ctgcttctcg tcgtcggtcg agcgcgcgag ctcgagcttc
     5461 tgcaccacgg cgtcgccgtg cggattacgg aacgtgttga cgccgacgat cggatacgac
     5521 ccgtcgtgct tgcgatgctc gtagtcgagc gactcctcct ggatcttgcc gcgctggtaa
     5581 ccggtctcca tcgcgcccag cacgccgccg cgctcggaga tcgcctcgaa ctccttcagc
     5641 acggcttcct cgacgagcat cgtcagctcc tcgacgatga acgagccctg gttggggttc
     5701 tcgttgttgc cgagccccca ctcgcggttg atgatcatct ggatggccat tgcgcggcgc
     5761 acgctctcct cggtcggcgt cgtgatcgcc tcgtcgtagg cgttcgtgtg cagcgagttc
     5821 gtgttgtcgt acgtcgagat cagcgcctgc agcgtcgtgc ggatgtcgtt gaacgcgatc
     5881 tcctgcgcgt gcagcgaccg gccgctcgtc tgcgagtgga acttgagctt ctggctgcgc
     5941 tcgttcgcgc cgtagcgctc gcgcatcgcc accgcccaga tgcgccgcgc gacgcggccg
     6001 atcaccgcat attccgggtc catcccatag ctgaagaaga acgacaggtt cggcgcgaag
     6061 tcgtcgatgt gcatgccgcg cgcgaggtac gcctcgacga acgtgaagcc gttggcgagc
     6121 gtaaacgcga gctgcgagat cgggttcgcg ccggcctcgg cgatgtggta gccggagatc
     6181 gacaccgaat agaagttctt cacgttgtga tggacgaagt actgctggat gtcgcccatc
     6241 accttgaggc tgaactcggt cgagaagatg caggtattct ggccctggtc ctccttcagg
     6301 atgtcggcct gcacggtgcc gcgcaccgtg gcgagcgtcc acgcgcggat cttcgcgacc
     6361 tcgtcgtcgg tcggctcgcg gccgttgtcc ccgctgaatt tgtcgagctg ctggtcgatc
     6421 gcggtgttca tgaacatcgc gaggatcgtc ggcgccgggc cgttgatcgt catcgacacc
     6481 gagttgttcg cggcgcagag gtcgaagcct gcgtacagca ccttcatgtc gtcgagcgtt
     6541 gcgatcgaca cgccggagtt gccgaccttg ccgtagatgt cgggacgcgg atccgggtcg
     6601 ttgccgtaca gcgtgaccga gtcgaacgcg gtcgacagcc gcttcgccgg catgccgtcg
     6661 gcgagcaggt gaaatcgctt gttggtgcga aacgcgtcgc cttcgccggc gaacatccgc
     6721 gtcggatcct cgttctcgcg cttgaacgcg aacacgccgg cggtgaacgg gaagctgccg
     6781 ggaacgtttt cggtcaactg ccagcgcagg atctcgccgt cgtcctcgta cttcgggagc
     6841 acgaccttgg gcaccttcgt gcccgacagc gacgtcgtcg tcagcgccgt gcggatttcc
     6901 cggtcgcgga tcttcacgat gtactcgtcg ccggcatagg cggccttcgt gcgcggccac
     6961 atgtcgagga gcttcttcga gcgtggatcg agccccgcct cgcgcccggc gatcagcgca
     7021 tcgagcgcaa ccaacgtagg gtcagagacg actttcggcg cggcgggttc ggagacattc
     7081 agcgacgcat tgccgaaagt cgtctctgac cctactttgg ttccgagcat cgccttcgcc
     7141 gcccgcaact gctgccgctc gcgcgcgatg ttcgactgcc gcccggccca ttcgtgatag
     7201 ccgcgcaccg tttcggcgat ctcggccaga tagcgcacgc gcgacgcggg aaccacggcc
     7261 tgccgctgcg tcgagcagcg cgtggtcagc gaggcgagcg cgcccttctt caccgcgagg
     7321 ccatgcgtct gcagccgctc cgcgatcgcc tggtagagcg ccgtgacgcc atcgtcgttg
     7381 aagcgcgagg cgatcgtgcc atagaccggc atctcgtcgg gcgccacgtt gaagagcgtg
     7441 cggttgcgct ggtactgctt gcgcacgtcg cgcagcgcgt cctgcgcgcc cttgcggtcg
     7501 aacttgttga tggcgacgaa attcgcgaag tccagcatgt cgatcttctc gagctgcgac
     7561 gccgcgccga actcggg
//
LOCUS       DASSVX010000358        16622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_482385, whole genome shotgun sequence.
ACCESSION   DASSVX010000358 DASSVX010000000
VERSION     DASSVX010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 16622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16622
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482385"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VFO53_15140"
     CDS             <1..1249
                     /locus_tag="VFO53_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877663.1"
                     /translation="STTGQLIPPVAITVSVKNESPPSTANSNINSFQFTVSGLALLNG
                     SGDIACPNARCSVDPSTGTVYVTNISPPVQAQQVMKVTFHASSCVAKSEAFISSNTVY
                     SGSQLNGDTFVAFGGKPDTDFPMVLTLSTRDAASPPTIADTGISCGPINCGQDFPVAD
                     SRTSCGINASNPICVTTRRGNDKNGAVCTSSPVDYFVTNLLEPNGKMHFKWETNSAGA
                     FAYRVNVPFPSTPITDPPTTPVPPPWQVSWLPTDGNAIFIGAATCVGADVSTFPASPD
                     GLPLPAQLTTLASAVKVNDKQIKVVSTPLGLPTGVFSIVIDGERMDVTKVNSNTWTVT
                     RGTGLTQATTHAVNKPVMSTPLPTLTSGQFSTDQNVLAVQQTVYSVGQQAQICRASAS
                     QDNGDDTWSAWFIDIGDGWVLGR"
     gene            1515..3317
                     /locus_tag="VFO53_15145"
     CDS             1515..3317
                     /locus_tag="VFO53_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877664.1"
                     /translation="MNGIRKLLLYITAIALVALALPGNAAPQKRFSLTMSMSTTTTAT
                     FNNLSNGNSYINSMNLTTPLPVTHVEVQANLITSTTSVTPNCTTAQPCAAGTTILISN
                     ISGVAPGTSGTLTLTVSAPADQNACSTSYTWSAMAFTGNAGNSGGGTSFLTPDGSTTA
                     TSTTSIACVLQFVNQPANAQVGSTITSVVNDPSAAPVKVQALIGGSSAANFTGQITLT
                     QSPATGALAGGSANASAGTAVFNSLSLDTLGTYTLTATATGFTSATSSPFKIFAGELN
                     CTEPFPTSIINPGNVAPDQPGYAFGNRNGSNKDGVTDGDCKPVLYTFTNNILASADPL
                     LSNTVNLSWDTQSQVNAAFQYTVNWQPVAVDSPASGWSTSPRPQVAWLDMSGNLATGA
                     TPSANIAWTPGLACVSSSLPVSYGTLAADLGTTVDANNTSTITINGIAAVPAQLNPAN
                     QPYSVPAAGSPDIPAVPFPVVIAGGIAGQQSTLTERMTAIAMVGSPVPNGNTFNITYQ
                     VRRGTVTEGSPTAQGVSTIESHGAGLSVMSTPLPIIPNNATVFKAPYKVNTQAQMCIA
                     KHGFTSFTIDAHGNTQVMYTTTIFDIGDGWVLGR"
     gene            complement(3385..7251)
                     /locus_tag="VFO53_15150"
     CDS             complement(3385..7251)
                     /locus_tag="VFO53_15150"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8877665.1"
                     /translation="MADGIVQREKTLEDFGRPERAGLYVPRILQQHLVDDPLRHAWSA
                     EGTAVFADVSGFTKLSEALAQKGREGAEQITETIENVFGVLLGVAYERGGTLLKFGGD
                     ALLLWFQGDHHVVRACGVAARIRDVLRDVGTIALPDVTITLRISQGVHTGRFEFFAVG
                     TSHRELLPVGPGWSRLAAAEASAGADQIVLTAETAASIPAEYLGDAMETGRMLLREPA
                     EHAEKLPLVPRPKLATETLARCLSTAIRQQVLAGGGGSEHRPVAIAFIKFSGTDKLIA
                     EHGADEAAAALHTIVSTVQAACDEQDVAFLASDVDADGGKLILAAGAPKATGNDEERM
                     LLALRKIVSTELPLPIRIGAHRGAVFAGDIGPAYRRTYTVMGDAVNLTARLMAKADVG
                     SIYATADLLDRSDTHFETVRLEPFSVKGKAAPVQAWSVGPGKGSKTRHVSLQKLPLTG
                     RNAELGVIRKAFVSARGGAGRLIDVVGEAGMGKTRLLEALRDAATGFRKIEATCEAYT
                     ASTPYAVWRELLRELMEFGRDDPDSAVIERVRGDLGAKAPELMPWLPLIAMTLGIEVE
                     PTPEMAMLAESNRRAKLHESLLQFLDLIFPKSCLVEIENAHHMDEASAELLSHVAGEL
                     GARPWLFAVAHRASASGFRAADAPTIVRVELKPLATQDALRMAQLATQQNPLPAHVLE
                     VVATRSGGNPQFLRDLLRTAIQSGGTADLPDSAEAAAMAQIDGLAPEDRALVRRAAVF
                     GLTFHPRMLSWLTEDGEEAPPVPASWERLHDLFEEEPDGYLRFRRSLLRDAAYEGLPY
                     KTRRRLHQVVAARLEAEMDFPEEAAGILSLHYFTAGEYRPAWRYARVAAERAEAAYAH
                     VEAAGMYARALDAGRQLPDLDNAEMAGVHQALGEVWYRASEFRKAADAYSAAQALVPD
                     DPLKQAALMLKLSYIEEKLGKYAEALRYTERSREMLDGLSGPEVARRLGESSSWCAML
                     LQFEGRTAEALEWAERAVVEAEAADDQDSLAEAYFVMGWAYGELGKEGAQRLMESSLA
                     AFQEAGNLVRQAGVLLSLGVVCQWEGRWDEALSFYERGRDANLKIGSVVGAALARINV
                     SEILTDRGEWGEAEALLRETLPLWKASQYRYYLAACLSLLGRVSLRLGRFDEALSRLE
                     EAKSNFLHVGAEEEVPAIDARIAECKVAMDRLDDGLAMVAELLGRAGESNAVTKVSSL
                     LQRVQGHALMKQGDLWGARDALEASLAAAREKHNLFEATLTMLSLIELDRLEGVEPPI
                     EMQNESRALLASLKVRAVPEVPVPVQ"
     gene            7331..7732
                     /locus_tag="VFO53_15155"
     CDS             7331..7732
                     /locus_tag="VFO53_15155"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET8877666.1"
                     /translation="MTQTPWQALADAARAACARAYCPYSRFPVGAAVLTADGSIASGC
                     NVENASFGLTCCAERNAIFRAIAGGATSIVALTLYTPTAQPAAPCGACRQVIMEFGRD
                     AQVRCICDGPGSIDCVAHELLPHAFTLAKPM"
     gene            complement(7779..10520)
                     /gene="metH"
                     /locus_tag="VFO53_15160"
     CDS             complement(7779..10520)
                     /gene="metH"
                     /locus_tag="VFO53_15160"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET8877667.1"
                     /translation="MPEPGVSSGAARAGRSSGRPLRLAGLEPLEIGDGSLFVNVGERT
                     NVTGSRAFAKLILGGDYTAAVEVARQQVQSGAQILDVNMDEAMLDSKAAMVRFLNLIA
                     SEPDIARVPVMIDSSKWDVIEAGLKCVQGKPVVNSISMKEGEAEFLRQAKLVRRYGAA
                     AVVMCFDEQGQADTYDRRIGIAQRAYGLLVERVGFPAEDIIFDPNVFAIATGIDEHSG
                     YAVDFIRATAWIREHLQHAKVSGGISNVSFSFRGNDAVRAAIHTVFLYHAIRAGLTMG
                     IVNAGQLGVYDELDPTLRTLVEDVVLHRPPSGGGNATEKLVAFAETYKVQGKRPEEDL
                     AWRDASVEARLSHALVKGITTFVVDDTEEARLAVAARGGRPIEVIEGPLMDGMNVVGD
                     LFGAGKMFLPQVVKSARVMKQAVAHLIPFIEAEKAASGSESRTKGRIVTATVKGDVHD
                     IGKNIVGVVLQCNNFEVIDLGVMVPAQKILATAREINADAVGLSGLITPSLEEMAHVA
                     AEMQREGFAVPLLIGGATTSRVHTAVKIAPNYTNGPTVYVPDASRAVGVVTSLLSADH
                     RDAFVAGVADDYAKIRVQHAGKRGPKLIPLAAARANAFAADWARYAPTVPAFIGARTL
                     RNVDLADIAGLIDWGPFFQAWELSGPYPAILDDAVVGEAARNLLAEGQAMLARIIGER
                     WLAADGVAGFWPAAAAGDDIVLWTDETRRTPLLTWRNLRQQNERPTGKPNYCLADFVA
                     GEASEVRDYVGAFAVTTGGGVEERVAAYKAANDDYSALMLKSLADRLAEAFAEWLHRK
                     VRTDWWGYARDEALDAAALVREAYRGIRPAPGYPACPDHAVKADLFEALHAGEAAMEI
                     TESYAMLPAASVCGFYLAHPDARYFAVGRIGEDQLVDFATRSGLDIGEARRRLAPNL"
     gene            complement(10513..11547)
                     /locus_tag="VFO53_15165"
     CDS             complement(10513..11547)
                     /locus_tag="VFO53_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET8877668.1"
                     /translation="MQPDHTAELTRLLRERILVLDGAMGTMIQQCRLSEHDFRGRFHD
                     HPHDLAGDNDILSLTQPSTVRSIHDAYFEAGADVVETNTFSATRIAQADYHMQDEARA
                     INESAARIAREAADRWTARTPGRPRFVAGALGPTNRTASISPDVNDPGARNVHFDELA
                     QAYGEAIDGLIDGGADLLLVETVFDTLNAKAALYAIDDAFERRGARLPIIVSGTITDA
                     SGRTLSGQTPEAFWNSVRHVRPLAVGLNCALGAALMRPYIEELSRIADTFVSCYPNAG
                     LPNPMSDTGYDESPEETSRMIAEFARSGFVNLVGGCCGTTPAHIRAIADAVRDIPPRR
                     LPSATELAVA"
     gene            11585..11971
                     /locus_tag="VFO53_15170"
     CDS             11585..11971
                     /locus_tag="VFO53_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET8877669.1"
                     /translation="MKHLKPRDASAFLHGNPHAVLIDCRSEMEYLFVGHPTGAQHVAW
                     NDGPDWDINPHFIGQVKKIASMNRPVVLICRSGQRSIAAGVELEKAGFTEVYNVLEGF
                     EGALDENHRRGTLGGWRKEGLPWEQT"
     gene            complement(11979..12494)
                     /locus_tag="VFO53_15175"
     CDS             complement(11979..12494)
                     /locus_tag="VFO53_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8877670.1"
                     /translation="MRAGVVMRGDVVIRDARDPADIPAIRRLFEAYAASLDVDLCFQN
                     FAHELAALPGDYAPPHGALLLAERGDVHVGCVALRPLRDEALRRIGEVKRLYVVPEAR
                     GTRTGHALMQALIARAQVGGYDELKLDTLVTMTSARTLYAALGFRPCAPYYPNPLPGV
                     GYLSLTLRPPS"
     gene            complement(12476..13111)
                     /locus_tag="VFO53_15180"
     CDS             complement(12476..13111)
                     /locus_tag="VFO53_15180"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET8877671.1"
                     /translation="MRAAVLLLIIVNLALFAYARLDRAAQSEAGHLREQVEPERIKLL
                     SPQAVAALGHAKVVCAEWGPFADVDRVRALAEIEPLAPARLVSQRAVSADTAYWVNVG
                     AMQSRAAAERRAGELRAQSITDLSVVDYPRGAFTVSLGVFRTEAAANARAETLAARGV
                     TGAQVEPRQQTGAQSMIVVRDAPEALVTRLKELQPQFAGADLRVGACAPAS"
     gene            complement(13299..14069)
                     /locus_tag="VFO53_15185"
     CDS             complement(13299..14069)
                     /locus_tag="VFO53_15185"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET8877672.1"
                     /translation="MSQILVIDVGNSRLKWALRGSRGWTARGAVANQDIGSLAVRDWQ
                     NLERPARAVGVNVAGEAARVRVEGQLARWRLPIEWLSARAEAGGVTNHYQVPSQLGAD
                     RWASLVAARRRAVAANDAAPQPCIVVNTGTAVTIDALDADGAFRGGLILPGVRLMLRA
                     LADNTASLRMAPGEFRDFPTTTADAIYSGAVQAICGAIGEMRRHLRGSRDEITCYLAG
                     GALQDVAPRVAAPVEVVDNLVLEGVLVLALLPRPPGGG"
     gene            complement(14116..15201)
                     /locus_tag="VFO53_15190"
     CDS             complement(14116..15201)
                     /locus_tag="VFO53_15190"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET8877673.1"
                     /translation="MLTPLGFSLLRQLSDGAFHSGEDLAATVGLTRARVSQLLKQAET
                     AGLALERVRGRGYRLLATPDFLDVKEIRAALEALDVEVAPPDAKAPLLPAANDDDLIF
                     SERVSTIPLHPAIAVEVTDQVDSTSTELLRRAARRDIHGALLAAEWQTAGRGRRGRVW
                     TAVAGGSLTFSLGWRFEQGAGFVAGLSLAVGVAVVRALEAEGLTGVELKWPNDLVHRH
                     LKVGGILIELNGDALGPSTVVVGVGLNIRLPQAVKRDIAQPVSDLTAVAGRGAPAIDR
                     NHLLARLVCELTASLSQYAAQGFVPFAAEWQRRHAYQGKPVKLLLPDGESVKGTVAGV
                     DSTGALVLADGPRRARFLAGEISLRRA"
     gene            complement(15317..16408)
                     /gene="hppD"
                     /locus_tag="VFO53_15195"
     CDS             complement(15317..16408)
                     /gene="hppD"
                     /locus_tag="VFO53_15195"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HET8877674.1"
                     /translation="MTGAPNGNLWDNPAGTDGFEFVEYAAPDPQALGRLFEQMGFTAI
                     AKHRSKDVLLYRQGTINFIVNAEPHSFAQSFARVHGPSICAIAFRVKDAAKAYAHCVA
                     NGAWGVEAHPGPMELNIPAIKGIGDSLIYLVDRYPTHDRTGVSIYDIDFVPLPGVDQH
                     PAGAGLTYIDHLTHNVHRGRMLEWSSFYERLFNFREIRYFDIEGQLTGLKSKAMTSPD
                     GGIRIPINESADDKSQIQEYLIDYRGEGIQHVALGTRDIYATVETLRTKGVAFQDTPQ
                     TYYEALATRLPGHREDTARLARNRILIDGAPAKDEMLLQIFTATVIGPIFFEIIERKG
                     NEGFGEGNFQALFESIEQDQIRRGVLKQT"
BASE COUNT         2845 a         5677 c         5391 g         2709 t
ORIGIN      
        1 ctcgacgacg gggcaattga ttccgccggt ggccatcacg gtgagcgtca agaatgaaag
       61 cccgccgtcg accgcgaact cgaacatcaa ctcgttccag ttcaccgtgt ccggcctcgc
      121 gctgctgaac ggcagtggcg acatcgcatg cccgaatgca cgctgcagcg tcgatccatc
      181 gaccggcacc gtttacgtca ccaacatctc gccgcccgtc caggcccagc aggtgatgaa
      241 ggtgactttc catgcgagca gctgcgtcgc gaagagcgag gcgttcatca gctcgaacac
      301 ggtttactcg ggctcgcagc tcaatggcga cacgttcgtg gccttcggtg gcaagccgga
      361 taccgacttt ccaatggtgc tcacgctttc cacgcgcgat gcggcgtccc cgccgacgat
      421 cgcggacacc ggcatctcgt gcgggccgat caattgtggc caggatttcc ccgttgccga
      481 ttcccgaacg agctgcggaa tcaacgcaag caatccgatc tgcgtgacga cgaggcgcgg
      541 caacgacaag aacggcgcgg tgtgcacgtc gtcgccggtc gattatttcg tgacgaatct
      601 cctcgagccg aacggcaaga tgcacttcaa atgggagacg aattccgccg gggcgttcgc
      661 gtaccgcgtc aacgtgccgt tcccgtccac accgatcacc gacccgccca cgacacccgt
      721 gccgccgccc tggcaagtct cctggctgcc gaccgatggc aacgccattt tcattggggc
      781 ggcgacgtgc gtcggcgccg atgtgtcgac cttccccgcc agtcccgacg gcctgccgct
      841 gccggcgcaa ctgacgacgc tcgcctcggc agtgaaggtg aacgacaagc aaatcaaggt
      901 ggtttcgacg ccgctcggcc tgccgaccgg cgtgttttcg atcgtcatcg acggcgagcg
      961 gatggacgtg acgaaggtca acagcaacac atggaccgtg acgcgtggga cggggcttac
     1021 ccaggcgaca acgcatgccg tcaacaagcc cgtaatgtcg acgccattgc caacgctcac
     1081 cagcggccag ttctcgaccg accagaacgt gttggctgtc cagcagacgg tttattcggt
     1141 gggccagcag gcgcagatat gccgtgcgtc cgcgtcgcag gataatggcg acgacacctg
     1201 gtcggcatgg ttcatcgaca tcggcgacgg ttgggtcctc ggtcgctagc cttttcgcgt
     1261 cgattcgcac caaggcggcc ccatcgggcc gccttttttc atattcacgg ctcgcccggt
     1321 tcggatgtcg aatttcttta cagtcgtcga tgaaccgttt cgtaatgcca gcagaaattt
     1381 gctcataatc agcgagttgc gtatgtacga cccggcggcc cgatttttgc acactccagc
     1441 aaccttgccg ccagtgcaat gctggcgtgc acctggcgca agcgcccttt aggattccga
     1501 ggaaggaaca agaaatgaac ggtatccgta agctgcttct gtacatcaca gcgatcgcgc
     1561 tcgttgcgct cgcgctgccc ggcaacgccg ccccgcaaaa gcgcttcagc ttgacgatgt
     1621 cgatgtcgac gaccacgacg gcgacgttca acaacctgtc gaatggcaac tcgtacatca
     1681 attcgatgaa cctcacgacg ccgttgcccg tgacgcacgt tgaggtacaa gcgaacttga
     1741 ttaccagtac gacgagcgtg acgccgaact gcacgacggc gcagccctgc gccgccggca
     1801 ccacgatcct gatctcgaat atttcgggcg tcgcgccggg aacctcggga acgctcacgc
     1861 tcaccgtgtc tgctcccgcc gatcaaaatg cgtgctcgac ttcgtatacg tggtcggcga
     1921 tggcgttcac cgggaacgcg ggcaactccg gcggtggtac gtcgttcctt actccggacg
     1981 gaagcacgac cgcgaccagc acgacgagca tcgcgtgcgt tctccaattc gtgaatcaac
     2041 ccgcgaatgc acaggtaggc tcgacgatca ccagtgtcgt caacgacccg agcgccgcac
     2101 ccgtcaaagt gcaggcattg attggcggtt catctgccgc aaacttcacg ggtcagataa
     2161 cgctcacgca aagccccgcg acaggagcgc tcgccggagg ctcggcaaac gccagcgcgg
     2221 gcacggcagt tttcaactcg ctttcactcg acacgctcgg tacctacacg ctgacggcca
     2281 ccgccaccgg ctttacgtcg gccacgtcca gcccattcaa gattttcgcc ggagagctga
     2341 actgcaccga gccattcccg acgagcatca tcaacccggg taatgtcgcg cccgaccagc
     2401 ctggctacgc cttcggcaac cgcaacggct cgaacaagga cggggtcacc gacggtgatt
     2461 gcaaacccgt gctctacacg ttcacgaaca acatcctggc gagcgcggat ccgttgcttt
     2521 cgaatacggt caacctgtcg tgggatacgc agagccaggt gaacgcggca ttccagtaca
     2581 cggtgaattg gcaaccggtc gctgtcgact cgcccgcgag cgggtggagc acgtcgccgc
     2641 ggccgcaggt cgcgtggctc gacatgagcg gcaacctcgc gacgggcgcg acgcccagcg
     2701 cgaacatcgc ctggacaccc gggctggcat gcgtcagtag cagcttgccg gtctcgtacg
     2761 gcacgcttgc agcggatctc ggcaccacgg tggacgcgaa caacacgagc acgattacca
     2821 tcaatggaat cgcggcagtg ccggcgcagc tcaaccccgc gaatcagccg tactcggttc
     2881 cggcggcggg ctcacccgac ataccggccg tgccgtttcc ggtcgtcatc gcgggcggga
     2941 tagccggaca gcaaagtacg ctcaccgaac gcatgaccgc gatcgccatg gtaggcagcc
     3001 cggtgccgaa cggcaacacc ttcaacatca cctaccaggt gcgccgggga acggtgacgg
     3061 aaggctcgcc taccgcgcag ggcgtctcga cgatcgagag ccacggggcc ggcttgagtg
     3121 tcatgagcac gccgctcccg atcatcccca acaatgcgac cgtcttcaag gccccgtaca
     3181 aggtcaacac gcaggcgcag atgtgcatcg ccaagcacgg gttcacgtcg ttcaccatcg
     3241 atgcgcatgg caatacgcag gtgatgtata cgacgacgat attcgacatc ggcgacggct
     3301 gggtgctcgg ccgatagcga acgttatgca acaaagcaaa agcggccctc gggccgcttt
     3361 tgctttgtgc gtcgaccgct gcgactactg cacaggaaca ggcacctcgg gcaccgccct
     3421 caccttgagg ctcgcaagca gtgcgcggct ctcgttctgc atctcgatcg gcggctccac
     3481 gccttcgagg cggtcgagct cgatcagcga gagcatcgtc aaggttgcct cgaagaggtt
     3541 gtgcttctcc cgtgcggccg cgagacttgc ttccagcgca tcgcgcgcgc cccacaggtc
     3601 gccctgcttc atcaacgcgt ggccctgcac gcgctgcagc agcgagctca ctttcgtgac
     3661 tgcgttcgat tcacctgcgc gaccgagcaa ttcagccacc atcgcgagcc cgtcgtcgag
     3721 cctgtccatc gcgaccttgc attcagcgat acgcgcatcg atcgccggca cttcctcttc
     3781 ggcgccgacg tgcaggaagt tcgacttggc ctcttcgagc cgcgaaagcg cctcgtcgaa
     3841 tcggccgagg cgcagcgaga cgcgaccgag gagcgacagg cacgcggcaa ggtaatagcg
     3901 gtactgcgac gccttccaca acggcagcgt ctcgcgcagc agcgcctcgg cttcgcccca
     3961 ttcgccccgg tcggtgagga tctccgagac gttgatgcgc gcgagcgccg ccccgaccac
     4021 gctgccgatc ttgaggttcg cgtcgcgtcc gcgctcgtag aacgaaagcg cttcgtccca
     4081 gcgcccttcc cactggcaga ccacgccgag gctcagcagc acgccggcct gacgcacgag
     4141 attgccagcc tcctggaatg cggcaagcga actctccatg agccgctgcg caccttcctt
     4201 gccgagctcg ccgtacgccc agcccatgac gaaataggct tccgcaagcg aatcctggtc
     4261 gtccgccgct tccgcctcga cgacggcgcg ttcggcccat tcgagcgctt ccgccgttcg
     4321 tccttcgaac tgcagcagca tggcgcacca cgacgacgat tcgccgagac gacgcgccac
     4381 ttcgggaccc gataatccgt cgagcatttc gcgcgagcgc tcggtatagc gcagcgcctc
     4441 ggcgtatttg ccgagctttt cctcgatata ggaaagcttg agcatcagcg ctgcctgttt
     4501 cagggggtcg tcgggaacga gcgcctgcgc agcgctgtac gcgtctgccg ccttgcggaa
     4561 ctcgcttgcg cgataccaaa cttcccccag cgcctggtgc acgccagcca tctctgcgtt
     4621 atcgaggtcg ggaagttgac ggcccgcatc gagcgcacgc gcgtacatgc ccgccgcctc
     4681 gacgtgcgcg taggcggcct cggcacgctc ggccgcaacg cgggcatacc gccatgccgg
     4741 gcggtactcg ccggcggtga aatagtgaag cgaaaggatg ccggccgcct cctcgggaaa
     4801 atccatctcc gcttcgagcc gcgcggcaac gacctgatgc aggcgacgcc gcgtcttgta
     4861 cggcagccct tcgtacgccg cgtcgcgcag cagcgagcgg cgaaatcgca ggtaaccgtc
     4921 cggctcctcc tcgaagaggt cgtgcaggcg ctcccagctc gccgggaccg gcggcgcctc
     4981 ttcgccgtcc tccgtcagcc acgacagcat cctcgggtgg aacgtgagcc cgaacaccgc
     5041 ggcgcggcga acgagcgcgc gatcctccgg tgcgaggccg tcgatctgcg ccatcgccgc
     5101 agcctccgcg gaatccggga gatcggcggt accccccgac tggatcgcag tgcgcagcaa
     5161 gtcgcgcagg aactgcggat tgccgcccga ccgcgttgcc accacctcga gaacgtgtgc
     5221 gggaagcgga ttctgctgcg ttgcgagctg cgccatccgc agcgcgtcct gcgttgcgag
     5281 tggcttgagc tcgacgcgca cgatcgtcgg cgcgtcggcg gcgcgaaagc cgctcgcgga
     5341 tgctcgatgc gcgaccgcga aaagccaggg acgcgcaccg agttcgccag caacgtgcga
     5401 cagcaactcg gcggaagcct cgtccatgtg gtgcgcgttc tcgatctcga ccaggcacga
     5461 cttcgggaaa atgagatcca ggaattgcaa cagcgactcg tgcagcttcg cgcgccggtt
     5521 gctctcggcg agcatcgcca tctcgggtgt cggctcgact tcgatgccga gcgtcattgc
     5581 gatcagaggc aaccacggca tcaattcggg cgccttggcg ccaagatcgc cgcgcacgcg
     5641 ctcgatgacg gcgctgtcgg gatcgtcgcg cccgaactcc atcaattccc gcagcagctc
     5701 gcgccatacc gcgtacggcg tcgatgccgt atacgcctcg catgtcgcct cgatcttgcg
     5761 aaatcccgtg gccgcatcgc gcagcgcctc aagcaggcgc gtcttgccca tccccgcttc
     5821 gccgacgacg tcgatgagcc ggccggcgcc gccgcgcgcg ctgacgaatg ccttgcgaat
     5881 gacgccgagc tcggcattgc gcccggtgag cggcagcttc tgcagcgaaa cgtgacgcgt
     5941 cttcgagccc ttgccgggac ccaccgacca ggcctgcacc ggcgcggcct ttcccttgac
     6001 gctgaacggc tcgagacgca cggtctcgaa gtgcgtgtcc gagcggtcga gcagatcggc
     6061 cgtggcgtag atcgacccga cgtcggcctt cgccatcagc cgcgccgtga ggttcaccgc
     6121 atcgcccatc accgtgtagg tgcggcgata ggcgggaccg atgtcgccgg cgaagaccgc
     6181 gccgcggtgc gcgccgatgc ggatcggcag cggcagctcg gtggacacga tctttcgcag
     6241 cgcgagaagc atgcgctcct cgtcgttacc ggtggccttc ggcgcaccgg cggcgaggat
     6301 cagctttccg ccgtcggcgt cgacgtcgga ggcgaggaat gcaacgtcct gctcgtcgca
     6361 ggcggcctgt acggtgctca cgatcgtgtg caacgccgcc gcggcttcgt ccgcgccatg
     6421 ctcggcaatc agcttgtcgg tgcctgaaaa cttgatgaat gcgatcgcca ccgggcggtg
     6481 ctcggaaccg cctcccccgg cgagaacctg ctggcgtatc gcggtcgaca ggcaccgcgc
     6541 gagtgtttcc gtcgcgagct tcggcctcgg caccagcggc agcttctcgg cgtgctccgc
     6601 gggctcgcgc aacagcatgc ggcccgtttc catcgcgtcg ccgagatact ccgcggggat
     6661 cgatgcggcg gtctcggcag tgagtacgat ctgatcggcg cccgccgacg cttcggcggc
     6721 cgcgagccgg ctccacccgg gacccaccgg cagcaactcg cgatgcgacg tgccgacggc
     6781 gaagaattca aaacggcccg tgtgcacccc ctgcgagatg cgcagcgtga tcgtcacatc
     6841 cggaagcgca atcgtgccga cgtcacgcag cacgtcgcgt atgcgcgcgg caacgccgca
     6901 cgcgcgcacc acatgatggt cgccctggaa ccacagcagc aaggcgtcgc cgccgaactt
     6961 gaggagcgtg ccgccgcgct cgtaggcaac gccgagcagc acgccgaaca cgttctcgat
     7021 cgtctcggtg atctgctcgg cgccctcgcg tcccttctgc gcgagcgcct ccgacagctt
     7081 cgtgaaaccg gaaacgtcgg cgaatacggc cgtcccttcc gcgctccacg cgtgccgaag
     7141 cggatcgtcg accagatgct gctggaggat gcgcggaacg taaagaccgg cgcgctcggg
     7201 gcggccgaag tcctcgagcg tcttctcacg ctgcacgatt ccgtcggcca cgatcgcgct
     7261 catcctgcat actgtaccgg tctctgaaaa cgcgcggcaa tccgaatttt catccggccg
     7321 gaacgcgaat atgacgcaaa cgccctggca agcgctggcc gacgccgcga gggcggcgtg
     7381 cgcccgggcg tattgcccgt acagccgctt cccggtcggc gcggccgtgc tcaccgcgga
     7441 cggctcgatc gccagtggct gcaacgtcga gaacgcgtcg ttcggcttga cctgctgtgc
     7501 cgaacgcaac gcgatcttcc gcgccatcgc cggcggcgcc acgtcgatcg tcgcgcttac
     7561 gctctacacg cccacggcgc agccggccgc gccgtgcggc gcgtgccggc aggtcatcat
     7621 ggagttcggt cgcgacgcgc aagtgcgttg catctgcgac ggcccgggca gcatcgattg
     7681 cgtcgcgcac gagcttctgc cgcatgcgtt cacgctggca aaaccgatgt agggtcagag
     7741 acgacttttc gaagagcgac gaaaagtcgt ctctgaccct ataaattggg cgcaagccgc
     7801 cggcgcgcct ccccgatatc gagcccggag cgcgttgcga aatcgacgag ctgatcctcg
     7861 ccgatcctgc ccacggcgaa atagcgcgcg tcgggatgcg cgagatagaa cccgcacacc
     7921 gaggcggccg gcagcatggc atagctctcg gtgatctcca tggccgcttc gccggcatgc
     7981 agcgcctcga acaggtcggc cttcacggcg tggtcggggc aggccggata gccgggcgcg
     8041 gggcgaatgc cgcggtacgc ctcgcgcacg agcgcggccg cgtccagtgc ctcgtcccgc
     8101 gcatagcccc accagtcggt acgcaccttc cggtgcagcc attcggcgaa cgcctctgcg
     8161 agtcgatcgg cgagcgattt cagcatcagc gccgaatagt cgtcgttggc cgccttgtag
     8221 gcggcgaccc gctcttcgac gccgccgccc gtggtcaccg cgaacgcgcc gacgtaatcg
     8281 cgcacctcgc tcgcctcgcc cgcgacgaag tcggcgaggc agtagttcgg cttgccggtc
     8341 ggccgctcgt tctgctggcg caggtttcgc caggtgagga gcggcgtccg ccgcgtctcg
     8401 tcggtccaca gcacgatgtc gtcgccggca gccgccgcgg gccagaagcc ggcgacgccg
     8461 tcggcggcaa gccagcgttc gccgatgatg cgcgcgagca tcgcctgccc ttccgcaaga
     8521 aggttgcgcg ccgcctcgcc gacgacggcg tcgtcgagaa tcgccgggta cggacccgaa
     8581 agctcccacg cctggaagaa cggcccccag tcgatcaggc ccgcgatgtc tgcaagatcg
     8641 acgttgcgca acgtccgtgc gccgatgaac gcgggcaccg tcggcgcgta gcgggcccag
     8701 tcggccgcga acgcattcgc ccgcgccgcg gcgagcggaa tcagttttgg cccgcgcttg
     8761 cccgcgtgct ggacgcgaat cttcgcgtaa tcgtccgcca cgccggcgac gaacgcgtcg
     8821 cgatggtcgg ccgacaacag gctcgtcacg acaccgacgg cgcgcgacgc atcgggaacg
     8881 taaacggtcg gcccgttcgt gtaattcggc gcgatcttca ccgccgtatg cacgcgcgac
     8941 gtggtcgccc cgccgatcaa aagcggcacc gcaaaaccct cgcgctgcat ttccgccgcg
     9001 acgtgcgcca tctcctcgag cgacggcgtg atgagccccg acaagccgac cgcgtcggcg
     9061 ttgatctcgc gcgccgtggc gaggatcttc tgcgcgggca ccatgacgcc gagatcgatc
     9121 acctcgaagt tgttgcactg gagcacaacg ccgacgatgt tcttgccgat gtcgtgcacg
     9181 tcgcccttga ccgtcgcggt gacgatgcgt cccttcgtgc gcgattcgct tcccgacgct
     9241 gccttctcgg cctcgatgaa cggaatcagg tgcgccaccg cctgcttcat cacgcgcgcg
     9301 ctcttcacca cctgtggcag gaacatcttt ccggcgccga agagatcgcc gacgacgttc
     9361 atcccgtcca tcagcggccc ttcgatcacc tcgatcggcc gcccgccgcg cgccgcgacc
     9421 gcgagacgcg cttcctcggt atcgtcgacg acgaacgtcg tgatgccctt gacgagcgca
     9481 tgcgaaaggc gcgcttcgac gctcgcgtcg cgccacgcga gatcctcttc gggccgcttc
     9541 ccctgcacct tgtacgtttc ggcgaacgca accagcttct cggtggcgtt gccgccgccg
     9601 gacggcggcc gatggagcac gacgtcctcg acgagcgtgc gcagggtcgg atcgagctcg
     9661 tcgtacacgc cgagctgccc cgcgttcacg atgcccatcg tcaggccggc gcggatcgcg
     9721 tgatacagga acaccgtgtg gatcgccgcg cgcaccgcgt cgttgccgcg gaagctgaag
     9781 ctcacgttcg agatgccgcc cgacaccttc gcgtgctgca ggtgctcgcg aatccacgcg
     9841 gtggcgcgaa tgaaatcgac ggcgtagccc gaatgctcgt cgatgccggt cgcgatcgcg
     9901 aacacgttcg gatcgaagat gatgtcctcg gccgggaagc cgacccgctc gacgagcagg
     9961 ccgtaggcgc gctgcgcgat gccgatgcgc cgatcgtacg tgtcggcctg gccctgctcg
    10021 tcgaagcaca tcacgacggc cgcggcacca tagcggcgca cgagcttcgc ctggcgcagg
    10081 aattcggcct cgccttcctt catcgaaatc gagttgacga cgggcttgcc ctgcacgcac
    10141 ttgagtcccg cttcgatcac gtcccacttc gacgagtcga tcatgacggg gacacgcgcg
    10201 atgtcgggct cggaggcgat cagattcagg aagcggacca tcgccgcctt cgaatcgagc
    10261 atcgcctcgt ccatgttcac gtcgaggatc tgtgcgccgc tttgcacctg ctggcgcgcc
    10321 acctcgaccg ccgcggtgta gtcaccgccg aggatcagct tcgcgaatgc gcgcgagccg
    10381 gtgacgttgg tgcgctcgcc gacgttgacg aaaagcgacc cgtcgccgat ctcgagcggc
    10441 tcgagcccgg caaggcgcag tggacgtccg ctgctgcggc cggcgcgcgc tgcgccggac
    10501 gaaaccccgg gctcaggcaa ccgccaactc cgttgcggaa ggcaggcggc gcggtggaat
    10561 gtcgcgcacc gcgtcggcga tggcacgaat gtgggcaggt gtggtgccgc agcagccgcc
    10621 gacgagattc acgaagccgc tgcgcgcgaa ctcggcgatc attcgcgacg tttcctccgg
    10681 cgactcgtcg tagccggtgt cggacatcgg gttgggcagg cccgcgttcg gatagcacga
    10741 gacgaaggtg tctgcgatgc gcgaaagctc ctcgatgtac gggcgcatca gtgccgcgcc
    10801 gagtgcgcaa ttgaggccca cggcgagcgg tcgcacgtga cgcaccgagt tccagaacgc
    10861 ctcgggcgtc tggcccgaca gcgtgcgccc cgacgcatcg gtaatcgtgc ccgacacgat
    10921 gatcggcagc cgcgcgccgc gccgctcgaa cgcgtcgtcg atcgcataga gcgccgcctt
    10981 cgcgttcagc gtgtcgaaca ccgtctcgac gagcagcaga tccgcgccgc cgtcgatcag
    11041 tccgtcgatc gcctcgccgt aagcctgcgc gagctcatcg aagtgcacgt tgcgcgcacc
    11101 gggatcgttg acgtccggcg aaatcgacgc ggtgcggttc gtcggcccga gcgcgccggc
    11161 gacaaagcgc ggacggccgg gcgtgcgcgc ggtccagcga tcggccgctt cacgcgcgat
    11221 gcgcgccgcc gactcgttga tcgcgcgcgc ctcgtcctgc atgtggtaat cggcctgcgc
    11281 gatgcgcgtt gcgctgaacg tgttcgtctc gacgacatcg gcgccggcct cgaagtacgc
    11341 gtcgtggatc gagcgcaccg tcgacggctg cgtcagcgac aggatgtcgt tgtcgccggc
    11401 gaggtcgtgc ggatgatcgt ggaagcggcc gcggaaatcg tgctcggaca gccggcactg
    11461 ctggatcatc gtgcccatcg cgccatccag gacgaggatg cgctcgcgga gcaggcgggt
    11521 gagttcggcg gtatgatccg gctgcatttt cgtactttat caaaccgcat tcgccgacac
    11581 ccggatgaag catctcaagc cccgcgacgc aagcgcgttc ctgcacggca atccgcacgc
    11641 ggtcctgatc gactgccgca gcgaaatgga gtacctgttc gtcgggcatc cgaccggtgc
    11701 gcagcacgtc gcgtggaacg acggacccga ctgggacatc aatccgcatt tcatcggaca
    11761 ggtgaagaag atcgcgagca tgaatcgccc cgtcgtgctc atctgccgca gcggccagcg
    11821 ctcgatcgcc gcgggtgtcg agctcgagaa agccggcttc accgaggtct ataacgtgct
    11881 cgagggcttc gaaggcgcgc tcgacgagaa tcatcggcgc gggaccctcg gcggctggcg
    11941 caaggaaggt ctgccgtggg agcagacctg agcccggctt acgaaggcgg ccgcaacgtg
    12001 agcgacaggt agccgacccc gggcagcggg ttcggatagt acggcgcgca tgggcggaat
    12061 ccgagcgccg catagagcgt ccgcgcgctc gtcatcgtca cgagcgtgtc gagcttgagc
    12121 tcgtcgtaac cgccgacctg cgcacgcgcg atcagcgcct gcatgagcgc gtgcccggtg
    12181 cgcgtgccgc gcgcctccgg cacgacgtag agccgcttca cttcgccgat cctgcgcagc
    12241 gcttcgtcac gcaacggtcg caacgcgacg catccgacgt gcacgtcgcc tcgctcggcc
    12301 agcaacaacg cgccgtgcgg cggcgcatag tcgccgggca gcgctgcgag ctcgtgcgcg
    12361 aagttctgga agcagaggtc gacgtcgagc gacgccgcat aggcttcgaa caggcgccgg
    12421 atagccggga tgtcggcggg atcgcgcgcg tcgcgaatca cgacgtcgcc gcgcatcacg
    12481 acgccggcgc gcacgcaccg acgcgaagat cggcgccggc gaactgcggc tgcagctcct
    12541 tcagtcgcgt gacgagcgcc tccggcgcgt cgcgcaccac gatcatcgac tgcgcgccgg
    12601 tctgctgcct cggctcgacc tgtgcaccgg tcacgccgcg tgcggcgagc gtctcggcgc
    12661 gcgcgttggc cgcggcttcg gtgcggaaca caccgagcga cacggtgaac gcgccgcgcg
    12721 ggtaatcgac gacggacaga tcggtgatcg actgcgcgcg caactcgccc gcccgccgct
    12781 cggccgccgc gcggctttgc atcgcaccga cattcaccca gtacgcggtg tcggcggata
    12841 ccgcacgttg cgaaacgagg cgcgccggcg cgagcggctc gatctcggcc agcgcacgca
    12901 cacgatcgac atcggcaaac ggcccccact cggcacaaac gaccttcgcg tgaccgagcg
    12961 ccgcaacggc ctgtggcgag aggagcttga tgcgctccgg ctcgacctgc tcgcgcaagt
    13021 gcccggcttc gctctgcgcg gcacggtcga ggcgcgcgta ggcgaacagc gccagattga
    13081 cgattatcag cagcaggacc gcggcgcgca tgaacccatc tcacaaatcg gctgtgcagg
    13141 ccgatagatg ttgtcgtttc ctcgctcgcc cctcgctgta cgtctgagta ctatctcggg
    13201 gctcgctgcg ggccttcgcg ctctcggccc gcacagccga tttttgagac aggttcgtcg
    13261 gtcgtggatg gggaccggcg ccggcgcacg gcgcgccgtc agccgccgcc cggcggccgg
    13321 ggcagcaagg cgagcacgag cacgccttcg agcacgagat tatccacgac ctcgacgggc
    13381 gctgccaccc gcggcgcgac gtcctgcagc gcaccgcccg cgagataaca cgtgatttcg
    13441 tcgcggctgc cgcgaaggtg ccggcgcatc tcgccaatgg cgccacaaat cgcctggacg
    13501 gcgcccgaat agatcgcgtc ggcggtggtc gtcggaaaat cgcggaactc gcccggcgcc
    13561 attcgcaacg aggccgtatt gtcggcgagc gcgcgcagca tcagccgcac gccgggcagg
    13621 atcaggccgc cacggaacgc accgtcggcg tcgagcgcgt cgatcgtgac cgccgtgccg
    13681 gtgttgacga cgatgcacgg ctgcggcgcg gcgtcattcg cggcgacggc gcgccggcgc
    13741 gccgcgacga gcgacgccca gcgatcggcg ccgagctgcg acggcacctg gtagtggttg
    13801 gtgacgccgc ccgcctctgc cctggcggac agccattcga tgggcaggcg ccagcgcgca
    13861 agctggcctt cgacgcggac gcgcgcggcc tcacccgcga cattgacgcc gacggcgcgc
    13921 gccggacgtt cgagattctg ccagtcgcgc acggcgagcg agccaatgtc ctgatttgcc
    13981 acggcaccac gggcggtcca gccgcgcgag ccgcgcaacg cccacttgag ccggctgtta
    14041 ccgacgtcga tgaccaggat ctgactcaag gcgagaacac gcgtgcgacg gcgcggtgat
    14101 cgcgccggtt gaatgtcagg cgcggcggag cgaaatttct ccggcgagga aacgtgcgcg
    14161 acgcgggccg tcggccagga cgagcgcacc cgtgctgtcg acgccggcga ccgtaccctt
    14221 gacgctctcg ccatccggca gcagcagctt cacgggcttg ccctggtagg catggcggcg
    14281 ctgccactcg gcggcgaacg gaacgaagcc ctgcgccgca tactggctga ggcttgccgt
    14341 gagctcgcag acgaggcgcg cgagcaggtg attgcgatcg atggccggtg caccgcgacc
    14401 ggcgaccgca gtgaggtcgc tgaccggttg ggcgatgtcg cgcttcaccg cctgcggcag
    14461 ccggatgttg aggccgacgc cgacgacgac ggtggaaggg ccgagcgcgt cgccgttgag
    14521 ctcgatcagg atgccgccga ccttcaggtg gcgatggacg agatcgttcg gccacttgag
    14581 ctcgacaccg gtcaagccct cggcctcgag tgcccggacg acggcaacgc cgacggcgag
    14641 cgaaaggccg gccacgaacc ccgcaccctg ctcgaaacgc cagccgagcg agaacgtgag
    14701 gctgccgcct gcgacggcgg tccagacgcg accgcggcgg ccgcgaccgg cggtttgcca
    14761 ttcggcggcg agcagcgcgc cgtggatatc gcggcgggcc gcgcggcgca gcagctcggt
    14821 cgacgtcgag tcgacctggt cggtcacttc gacggcaatg gccgggtgca gcggaatcgt
    14881 ggaaacacgt tccgagaaga tgagatcatc gtcgttggcc gcgggcagca gcggggcctt
    14941 ggcgtcgggc ggcgcgactt ccacgtcgag cgcttcgagc gccgcgcgga tttccttgac
    15001 gtcgaggaag tcgggcgtgg cgagcagccg atagccgcgg ccgcggacgc gctcgagcgc
    15061 gagaccggcc gtttcggcct gcttcagcag ctgcgaaacg cgggcgcgcg tcagcccaac
    15121 cgtcgcggca agatcttcgc ccgagtggaa ggcgccgtct gaaagctggc gcagaagcga
    15181 gaagccaaga ggagtaagca ctaacctaca cgcccgtcgg aaacgttgaa ttctagcaga
    15241 ctcgacgtgc aattgcacga tttttgtctg gagttcaacg gctggtgaat ggcatgtgcg
    15301 gccgcagcaa tccctttcaa gtctgcttca gcacgccgcg ccggatctgg tcctgctcaa
    15361 tggattcgaa cagcgcctgg aagttgcctt cgccgaaacc ttcgttgccc ttgcgctcga
    15421 tgatctcgaa gaaaatcggc ccgatcaccg tcgccgtgaa aatctgcagc agcatttcgt
    15481 ccttggcggg cgcaccgtcg atcaggatgc ggtttcgcgc gagccgcgcg gtgtcctcgc
    15541 ggtggccggg aagccgcgtc gcgagtgctt cgtagtaggt ctgcggcgtg tcctggaaag
    15601 cgacgccttt cgtgcgcagc gtttcgacgg tcgcgtagat gtcgcgcgtg ccgagcgcca
    15661 cgtgctggat gccctcgccg cgatagtcga tcaggtactc ctggatctgg ctcttgtcgt
    15721 cggcgctttc gttgatcgga atgcggatgc cgccgtcggg gctcgtcatc gccttgctct
    15781 tgagcccggt caactggccc tcgatgtcga aataacggat ctcgcggaag ttgaacagac
    15841 gctcgtagaa gctcgaccat tcgagcatgc gtccgcgatg cacgttgtgc gtcaggtgat
    15901 cgatgtaggt gaggccggcg ccggccggat gctgatcgac gccggggagc ggcacgaagt
    15961 cgatgtcgta gatcgatacg ccggtgcggt cgtgcgtcgg atagcggtcg acgaggtaga
    16021 tcagcgaatc gccgatgccc ttgatcgccg ggatgttgag ctccatcgga cccggatgcg
    16081 cctcgacgcc ccacgcgccg ttggcaacgc agtgcgcata cgccttcgcg gcgtccttta
    16141 cgcggaaggc gatcgcgcag atgctcggcc cgtgcacgcg cgcgaacgat tgcgcgaacg
    16201 agtgcggctc ggcgttgacg atgaaattga tcgtcccctg ccggtagagc agcacgtcct
    16261 tcgagcggtg cttcgcgatg gcggtgaagc ccatctgctc gaagaggcgc ccgagcgcct
    16321 gcgggtcggg cgccgcgtat tcgacgaact cgaacccgtc ggtgcccgcc gggttgtccc
    16381 acaggttgcc gttcggtgcg cctgtcatct cgacctcgct tgagcaagta gggtcagaga
    16441 cgacttgtgt tcaccgacgg atacttccga tgttcgaaga acaagtcgtc tctgacccta
    16501 ctttctcata acgcgaggag gctccgctcg gcatcggccg acagaacgtt cttccaggcg
    16561 cccgcgttgt cgcgcgtcgc gagccacgcc gcggtgtcgc gaatggtgtc ggcgagtgga
    16621 cg
//
LOCUS       DASSVX010000359         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_732522, whole genome shotgun sequence.
ACCESSION   DASSVX010000359 DASSVX010000000
VERSION     DASSVX010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732522"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="VFO53_15200"
     CDS             <1..1877
                     /locus_tag="VFO53_15200"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET8877675.1"
                     /translation="LGEVAEEAGGVRSIRFVNIREAAGWSAEAPHATPKIAALLAAAA
                     LPDPEPVPIVNYKSEGQVLIVGPLDVALRWAEVLRVSLSVTVLGTGASQHASLPGARE
                     MAVFSGELTHLDGWLGAFDAKWSQLNPIDLELCTRCNACVTACPEQAIGRHFQVDLDR
                     CREHRDCVVACGVVGAIDFERRDTARSGRFDTVLDLSPAPWFRQHQPPQGYFAPGADP
                     FAQARAVAELVLAVGEFEKPKFFAYKPSICAHSRSEKTGCTQCIDVCSTLAIRADGDH
                     VAVEPHLCMGCGACTTVCPSGAITYAYPAAADLGARMRTLLASYARAGGRDAGLLLHA
                     ADSEALLESLARNGRGLPARLMPVAVEHIASVGIDLWMAALAWGASSIAVLATGREAP
                     QYLDALRFQMGIAQTIANAQGYQGEHLRLIDASAGDIGRALWEWPVALPPRIAATFAA
                     TNDKRRTLAHAFEHLAQHAPVPQTTIALPAGAPFGALAVDRERCTMCLACVGSCPEAA
                     LLDHAETPQLRFIESNCVQCGICVATCPEQAISLVPRLDLSPDARAPRVLNEAKVFAC
                     VRCGKPMGTEKLIGAMLERLRTHSMFSAAGAIERLKMCADCRVVDLMTNESPADIRNL
                     "
     gene            1918..2622
                     /locus_tag="VFO53_15205"
     CDS             1918..2622
                     /locus_tag="VFO53_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HET8877676.1"
                     /translation="MRAAEPPQGANCSPVGGSEAAKPRAWGDHTSAQTIALAHPLSPE
                     DRARADFYALLARLYADGPDARLLQTIARADTIGGDSSFARAWNRLVAASGAMDAEAA
                     AQEYTDLFVGVGKCEVNLHGSHWLTGYMMEKPLVELRRELAALGIARRADVTMLEDHL
                     SAMCETMRLLVAGHEDREPVPVATQRAFFERHIGCWVFSCCTAMRETPIANYYACVGE
                     FTEQYMALDRDALAMD"
     gene            2668..2907
                     /locus_tag="VFO53_15210"
     CDS             2668..2907
                     /locus_tag="VFO53_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HET8877677.1"
                     /translation="MSDASHRDISPAATVAVPALDSKRRRFLMAFGAGAAGAASASAL
                     AAAPAAVIEATQPAPASQGYRETEHVRNYYATTRI"
BASE COUNT          468 a         1034 c         1086 g          521 t
ORIGIN      
        1 tgctgggcga ggttgcggaa gaggcgggcg gcgtgcgctc gatccgcttc gtcaacattc
       61 gcgaggcggc cggctggtcg gccgaagcgc cgcacgcgac accgaagatc gcggcgctgc
      121 ttgccgcggc ggcgctgccg gatccggagc cggttccgat cgtcaactac aagtcggaag
      181 ggcaggtgct gatcgtcggt ccgctcgacg tggcgctgcg ctgggccgag gttttgcggg
      241 tgtcgctgtc ggtgaccgtc ctcggcaccg gggcgtcgca gcatgcgtca ttgccgggtg
      301 cgcgcgagat ggcggtgttc tccggggagc tcacgcacct cgacggctgg cttggcgcgt
      361 tcgacgcaaa gtggtcgcag ctcaatccga tcgacctcga gctttgcacg cgctgcaacg
      421 catgcgtgac cgcctgcccc gagcaggcga tcggccgcca tttccaggtc gatctcgacc
      481 gctgccgcga acatcgcgac tgcgtggtgg cgtgcggggt cgtcggagcg atcgacttcg
      541 agcgccgcga cacggcacga agcggccgct tcgataccgt gctcgacctg tcgccggcgc
      601 cgtggttccg ccagcaccag ccgccccagg gctatttcgc gcccggtgcc gatccgttcg
      661 cgcaggcgag ggcggttgcc gagctcgtgc tggcggtggg cgagttcgag aagccgaagt
      721 tttttgcgta caagccgtcg atctgcgcgc acagccgctc cgaaaagacc ggttgcacgc
      781 agtgcatcga cgtctgctcg acgttggcca tccgtgccga cggcgatcac gtcgcggtcg
      841 agccgcacct ttgcatgggc tgcggcgcgt gcacgaccgt atgcccgtcc ggcgcgatca
      901 cgtacgccta cccggcggcg gccgacctcg gcgcgcggat gcgcacgctg ctcgcgagct
      961 acgcgcgggc gggcggccgc gacgcgggcc tgcttctgca cgcggccgat tcggaggcgc
     1021 tgctcgagtc gctcgcgcgg aacgggcgcg gactgccggc gcgcctgatg ccggttgccg
     1081 tcgagcacat cgcctcggtc ggcatcgact tgtggatggc ggcgctggcg tggggtgcgt
     1141 cgagcatcgc agtgctcgcc accggccgcg aagcgccgca atacctcgat gcgctgcgtt
     1201 tccagatggg gatcgcgcag acgatcgcca acgcgcaggg ctaccagggc gagcatttgc
     1261 gtctgatcga cgccagcgcc ggcgatatcg gtcgtgcgct gtgggagtgg ccggtggcgc
     1321 tgccgccccg gattgcggcg acattcgcgg caaccaacga caagcggcgc acgctcgcgc
     1381 atgcgttcga gcatctggcg cagcacgccc cggtgccgca gacgaccatt gcgctgcccg
     1441 ccggcgcacc gttcggcgcg ctcgcggtcg atcgcgagcg ctgcacgatg tgtctcgcct
     1501 gtgtcggctc ctgtccggag gcggcgctcc tcgatcacgc cgagacgccg cagctgcgct
     1561 tcatcgaaag caattgcgtg cagtgcggca tttgcgtcgc aacatgcccg gaacaggcaa
     1621 tttcgctcgt gccgcggctc gatctttcgc ccgacgcgcg cgcgccgcgt gtgctgaacg
     1681 aggcgaaggt gttcgcgtgc gtgcgctgcg gcaagccgat gggcaccgaa aagctcatcg
     1741 gcgcgatgct cgagcggctg cgcacgcatt cgatgttttc ggcggccggt gccatcgagc
     1801 ggctcaagat gtgcgccgat tgccgtgtcg tcgacctgat gaccaacgag tcgcccgcgg
     1861 acatccgcaa tctttgagca tgagcgcgcc gggccgccgc aagcgtaggg gaatcgcatg
     1921 agagccgcag agccgcctca aggcgcgaat tgctcccccg tggggggcag cgaagcggcg
     1981 aagccgcgag cgtgggggga tcatacaagc gcgcagacga tcgcgcttgc gcatccgctg
     2041 tcacccgagg accgcgctcg cgccgatttc tacgcgctgc tggcgcgcct ttacgccgac
     2101 ggtcccgatg cgcgattgct gcagacgatc gcgcgggccg acacgatcgg cggcgattca
     2161 tcgttcgcgc gcgcctggaa tcgcctcgtc gccgcgagtg gcgcaatgga cgccgaggcg
     2221 gcggcgcagg aatacaccga tcttttcgtc ggcgtcggca agtgcgaggt gaacctgcac
     2281 ggttcgcact ggctgaccgg ctacatgatg gaaaagccgc tggtggagct tcgacgcgag
     2341 ctggcggcgc tcggtatcgc gcgtcgtgcc gatgtcacga tgctcgagga tcacctgtcc
     2401 gcgatgtgcg agacgatgcg gcttctggtc gccgggcacg aggaccgcga gcccgttccc
     2461 gttgcaacgc agcgcgcgtt tttcgagcgc cacatcggat gctgggtctt ttcttgctgc
     2521 accgcaatgc gtgaaacccc tattgccaac tactatgcct gcgtaggaga attcacggaa
     2581 caatacatgg cgctggaccg cgacgcactc gccatggact aacgatcatg cgcgcggaat
     2641 cagtttggaa tcaccaggag gagcaccatg agcgatgcat cgcaccgcga tatttcgccg
     2701 gcggccaccg ttgccgtccc tgcacttgac tcgaagcggc gccggttcct gatggcgttc
     2761 ggcgccggcg ctgcgggtgc cgcgtccgcg tcggcgcttg ccgccgcgcc ggcggcggtc
     2821 atcgaagcga cgcagcccgc gcccgcatcg cagggctacc gggaaaccga gcacgttcgc
     2881 aactattacg cgacgacccg catctgatcg tggcgagtgc gctcccccgc ggagctgttt
     2941 gatcgaattc gcgtcaatcc acttctcaag caggagaagc atcatgctgc tcacgcgcaa
     3001 gtccgatagc gcccgtcagg cgggaccgcg gctgtcccga ggcatcggcg cgaggatcgc
     3061 gacgatggac cgtcgcgcgt tcctgaagcg ctccggcatg gtcgccggc
//
LOCUS       DASSVX010000360        24930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1298466, whole genome shotgun sequence.
ACCESSION   DASSVX010000360 DASSVX010000000
VERSION     DASSVX010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 24930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24930
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298466"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VFO53_15215"
     CDS             <1..790
                     /locus_tag="VFO53_15215"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET8877678.1"
                     /translation="AAGLTRFILVTGRNKRAIEDHFDRAYELEHELAARESDVALREL
                     RSAVPSGVTFSYVRQREASGLPDALRSARSLIGQAPFAVILPDDLIDAPRPALAQLLD
                     VYDEQQVSVAGVQRSAREASDNGCLIAADLNTSKLRAARELVKVPPIGAASGPVSLCG
                     RYVFTSAVWDAMQDERDPMLAKTVRALVERERVFACLVDGQRFDCGTKLGFLEAQFAF
                     AQKHLELWPGLRRRLDHLLADKAAPVEAPTAASSTGAVKAAIMR"
     gene            951..1154
                     /locus_tag="VFO53_15220"
     CDS             951..1154
                     /locus_tag="VFO53_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877679.1"
                     /translation="MAGNTEPSLPRVTNASDAHLHRNPDEGRTWPRCEPAFTHFELGA
                     SSELRPTALMAKRYTPRYGVRKE"
     gene            1205..1624
                     /locus_tag="VFO53_15225"
     CDS             1205..1624
                     /locus_tag="VFO53_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET8877680.1"
                     /translation="MGVGDVDSRYRTGDDYWTDRNGGPGPQVMAADTLQGDSVRNDAG
                     EDLGEITDIMLDVPTGRVAYAVMSVGGVLGIGDKLFAIPWSALRLDTENKCFRMNVAK
                     ERFEQAPGFDKDHWPTMADQRWAETVHSFYNARPYWS"
     gene            1785..2963
                     /locus_tag="VFO53_15230"
     CDS             1785..2963
                     /locus_tag="VFO53_15230"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HET8877681.1"
                     /translation="MVTHTTGPVRNGNGNNGHGGNVKADAPDPGLRATLKKASLRYSR
                     STDRGLRREGTPETFRYVDGDGRKVRASETLARIRMLAIPPAWTSVWICGDPQGHLQA
                     TGRDARGRLQYRYHAQWRAQRDEHKFDRLAEFAASLPAIREAVARDLEQPGLPRRKLL
                     AAMVRLLDRTYVRVGDERYRRDNGSFGLTTLRTRHVQVRGDRIRLRFRGKSGKEHDLS
                     LADRRLARMVRRCLDLPGYELFRYVEDGETRPLCATDVNEYLQEIAGADYTSKDFRTW
                     GATVIATVLLTRFGLPGSVSERTKTVNRALRAAAQALGNTLAVCRKSYVHPGVVEAYL
                     GEALPPRRVRIEASGLRAGERRTLALLRTLRGPVKRARHRPRTRSADPSGIQAAPPAP
                     "
     gene            complement(2866..3690)
                     /locus_tag="VFO53_15235"
     CDS             complement(2866..3690)
                     /locus_tag="VFO53_15235"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET8877682.1"
                     /translation="MAIAIHDVSPATWRECRELLAMLDNAGAGPVTLLVIPRHHHRTS
                     VLREHAFVRAMDARLARGDELVLHGYFHIDDAPAPRTLRQWFARRVLTRTEGEFAAID
                     AEAAASRIARGVALFDRLGWPLAGFAPPAWLLGDGARSALSHCAHAFEYVTVRSGIYH
                     LPAWRFERTANLCYSPDSALRRAVSRAAIGHELRRANALPLLRISLHPQDVRGAGVLR
                     HWQRLIADALAGRTPVTKCQWVRLFRAPEAPPGCRTGPRFVSGDDAARASPAHAAS"
     gene            complement(3707..4828)
                     /locus_tag="VFO53_15240"
     CDS             complement(3707..4828)
                     /locus_tag="VFO53_15240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8877683.1"
                     /translation="MHLLDVTMLYAPTSGGVVRYLRAKHAWLRRHTSIRHSLLVPGAA
                     DARGASGEIFLRTRTLGRQGHRWPFAAHRWSRQIRELAPDLIEVGDAGPEGWAALHAA
                     RSLDVPLIAFCHSDIARMATRRLGALPGAAVQLYAREFYRRCDLVAAPSEFMRAQLNA
                     WGVERVVARPLGVDVATFSPRRRVPGLRRELGLPARARLLAYAGRFSTEKNLPVLLDA
                     FRALGPHYHLVLAGPQIELALPANVTLLPFLHSSLELARILASVDGLVHAGDQETFGL
                     VVLEAMACGRGVVAANAGALPEIVTPADGVLVTPGDPAALAEGVRAFYRRDPDTLGRA
                     ARARAESEYSWSTAMRGLLGLYRGAIASASARTPRYATP"
     gene            5002..5328
                     /locus_tag="VFO53_15245"
     CDS             5002..5328
                     /locus_tag="VFO53_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877684.1"
                     /translation="MPLFASFGVVDVGADELELDDELVSGDAGGVLALDDDVLGVDDG
                     LDDIDPLDDEGGVVDEEDDVEGDGVTTGGVVPGVVDDSRLQPAMPSTSPVQSSVTNAL
                     FIAISR"
     gene            5651..7075
                     /locus_tag="VFO53_15250"
     CDS             5651..7075
                     /locus_tag="VFO53_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET8877685.1"
                     /translation="MPHALIVDDDTHAAEALAELVAENGFTTACADSLQGARDLLSTS
                     PDVILLDLILPDGNGLELLRDATTRESETQVVVITGHATLESSIEAMRHGATDYLLKP
                     INLAQLRGVLSRVARPPELSAEVKDLRTELRSLGHFGRLVGASAPMQRLYDQLSRVAP
                     TNATVFITGESGTGKEMVAQTIHEVSRRRLRPFLAVNCGAISPQIIESELFGHEKGSF
                     TGANRQHRGYFERAHGGTLFLDEITEMPMDLQVKLLRVLETQTVSRVGSDRLIEIDVR
                     VLAASNRNPHDAVAAGKLRKDLLYRLQVFPLYVPPLREHSDDIELLAAHFLADLNRRS
                     NTNKAFTPAAIDRLKRYHWPGNVRELSNAVHRAYIMTDGDWITQVGLAPEPTLSADFS
                     GGRSFQVGVGDRIDAMEKRLILATVQHTHTKEAAAEILGISVKTLYNRLRAYESGADL
                     RADAAGDAPAAASPASPGSRTPAT"
     gene            complement(6979..7449)
                     /locus_tag="VFO53_15255"
     CDS             complement(6979..7449)
                     /locus_tag="VFO53_15255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8877686.1"
                     /translation="MHTLPRSNLKLLIVDDDPTTRALLAEALETRGACVRASASAREA
                     QQTLVVWHPDLMISDVGMPRESGYELIRRVRHLPAEEGGSTPAIACTGYARAEDRARA
                     IDAGFDAVVAKPVDLDLLVSTIAHVAGVRDPGEAGDAAAGASPAASARRSAPDS"
     gene            7489..8568
                     /locus_tag="VFO53_15260"
     CDS             7489..8568
                     /locus_tag="VFO53_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877687.1"
                     /translation="MNARNVLQESGLPDSRLLARLLQAGTLVLDEDDTLTFASAGACD
                     LLGVDDEAALREHWSELRAQLRVDRWPRTLPDGDAYRGRADVLTPAGPRAIRFEMHGM
                     ADAGSLHRAVLVRDRAHVLATDSALLLASEAQANRHVLTGLVHAAKGPLNNFNLTLAL
                     LSAGIARGDALAATPEAAARRTRYIDVLQKEATRLASCIDEIHALTVRHEPSWEPIDL
                     AATSIDCARVLRHGATMREVSLELAVPGQPVMAIGDPQLVRLALLSFTICVLELTPAG
                     GRVAWRVMHDRDTHAPSVAITTSQPVLPRALATALFRLSCMAESDYSGAIAARLIVEA
                     QGGEVILHDAGDGAAGILLHIPGHA"
     gene            8813..10075
                     /locus_tag="VFO53_15265"
     CDS             8813..10075
                     /locus_tag="VFO53_15265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8877688.1"
                     /translation="MISEHASPLATIGGVDAGGQNIYVAHTARHLAALGYAVDIFTRR
                     DSEALPDVVDWMPGIRVVHVRAGPASFVRKESLLPHMAQFARSVVDHAKSARDRGAPY
                     AVCHAHFFMSGLVARRLKQALGIPYVVTFHALGRVRLLHQPHDEFPPQRCILEQMVID
                     EADAIVAECPQDEMDLQRHYRTPASRMRMIPCGFDPAELQPVARAKARAHLGLPGERP
                     IVLQLGRMVPRKGVDTVIHALGVLERRYRLNPLLLIVGGESPTPDPVRTPEIGRLQRI
                     AQDEGVAADVRFVGQRPRCELRYFYSASDVFVTMPWYEPFGITPVEAMACGVPVIGAR
                     VGGVQYSVEDGRTGFLVEAKNADALARRLAHVFSDASIPRLLGKRARRRAWERFTWER
                     ISRSLADLYAEVATAASLPRPALAQGTR"
     gene            10085..10807
                     /locus_tag="VFO53_15270"
     CDS             10085..10807
                     /locus_tag="VFO53_15270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8877689.1"
                     /translation="MDTDLTQKVVVVTGGGQGLGAAICSELGQAGANVFVADRSERAE
                     GVAAALTRDGQRAYALRGDVGSPDDVRRFVDEAKKRFGRLDAVINNAGIDRTTGVDEL
                     DVDDWDAIIRTNLRGPFLLSKFALPLLRDSQGDIVNVASTAAKRGWPNASAYHASKWG
                     LLGLSYAMHAEFRAHKVRVTAVIAGGMRTPFLLERFPDLDPAKLQDPRDVARAVRFAL
                     SQPRESVVAEITVLPVEESSWP"
     gene            10798..11454
                     /locus_tag="VFO53_15275"
     CDS             10798..11454
                     /locus_tag="VFO53_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004530684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET8877690.1"
                     /translation="MAMNGASHQPQAWDVGPAAAVRDATAIFIDKDGTLVHDVPYNVD
                     PARIELVPGAGDALRALQDAGFLLFVVSNQPGVALRRFPMRALRTVEARLDELLAPFG
                     VMFTAYCWCPHPPAGVRAAIACTCRKPRPGLLLDAGATHGVDLERSWMIGDILDDIEA
                     GNRAGCRTVLVDRGNETRWRTGRLRTPNAIVYQLADAAEIILDGARDQSAARPRAAPA
                     "
     gene            11451..12596
                     /gene="waaF"
                     /locus_tag="VFO53_15280"
     CDS             11451..12596
                     /gene="waaF"
                     /locus_tag="VFO53_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HET8877691.1"
                     /translation="MITMDPSWRSARSLLAVRLDGIGDLLMTTPALRALKQDRRGRSL
                     TLLTSPAGAIAARELPFVDDVIVFTAPWMKAAEAVPVSPVATASLVEQLRERAFDAAI
                     VFTVFTQSALPSATLLHLAGIPRRAAYSRENPYALLTDWRADPDRDAHAGVRHEVQRQ
                     IDLVQSLGTRVRDTRLSFPVREDARRSMLACVVAAGMEPGRPWLIVHPGATAASRRYP
                     HDKLADAIGRLQQRGRWQIAVAGTAEDRECARRIAASVNGVVPLTGVLDLAELAALLQ
                     AAELVVCNNSAPAHIAAAVGTPVVDLYALTNPQHTPWGVAHRVLSHDVDCKYCLKSAC
                     PQRHHRCLADVAPGEIVAAVDALLPDAPVRVPPRVRAFAGAGAGAGA"
     gene            12605..14332
                     /locus_tag="VFO53_15285"
     CDS             12605..14332
                     /locus_tag="VFO53_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HET8877692.1"
                     /translation="MRTLGINAAFHDSAACLVDDGAVVAAAEEERFTQIKHGKRPVPF
                     SAWELPFHAIDYCLAEAGIDLDAIDHVAYSFEPGLFVNGSGMPSQITLPLEPSAIRAP
                     AGKAPWDPLFLSYVVNAPRQLSGGAPHHLQQRLRSRVNAPHWQWHYVPHHLAHQASAF
                     LAAPFDECAVLTLDGRGENTSTQYGAFRDGRYTRISEVPLPHSLGLLYEDVTRHLGFL
                     HSSDEYKVMALASFGTPRFVDALREHVRWQGDGRYVVDAPPFASFCAPRKPGEPFLPV
                     HADVAHATQRVLEETVLGIVRWLRATTQLPNLAMAGGVALNCVMNAALRDAAVFDRIW
                     VQPAAGDAGTALGAALWIDREARGGDAAKRAFRMDHAYLGPGYSDAEIETALRHARAR
                     YEAPADLPGTIAAQLERDRIVGWFQGRMEFGPRALGARSILASPLHASMQARLNELKD
                     REDFRPVAPAVLEEAASDWFENCTSSPFMLFVHRVPPARRARIPAACHADGTARVQTV
                     SRDANPLYHALIGQFARRTGVPVVINTSFNTRGKPIVCSPRDALECFFTSPLDVLAIG
                     RYVIEKGAH"
     gene            14329..15312
                     /locus_tag="VFO53_15290"
     CDS             14329..15312
                     /locus_tag="VFO53_15290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8877693.1"
                     /translation="MNPEVSVVIATYERRDLLLRCADALLAQTLAAARFEIVIVDDGS
                     SRATREYVMAELSSRRRLRRAPTLRFLWLPSNRGPAAARNCGILAARGGVIAFTDDDT
                     VPHANWLESGLDAIRHGADAVAGAVEVPLPAVPTDYERDAAGLSNAGFVTANCFVRRD
                     LLCAVGGFDERFRCAWREDTDLFFRLRASGAIVRASHAARVVHPVRPARWGVSLAQQR
                     KVMFDALLYKKFPQGYRRYVRARPPWDYYCAVVAGLCALVNAVAGFWLLALACLVAWL
                     GISVAFASRRLADTSHAPRHVAEMIVTSLLIPWVSVYWRTRGALRFRVPFV"
     gene            15329..16366
                     /locus_tag="VFO53_15295"
     CDS             15329..16366
                     /locus_tag="VFO53_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET8877694.1"
                     /translation="MTPLDAVLAPAVAWPRTPPLPRFVQIEPVGQCNLRCQMCPIQFR
                     KDGPPHGPLAFLAFDDFTRLVEGFGDIDELQLQGLGEPTMHPQFFAMVAWASARGIRV
                     STNSNLTLWSERRARQCVDSGLATLHVSLDAATPELYERIRCGAHFPKVIRNLRRVMA
                     ARRARQSPLHVRVVMVLMRQNLHELPALVRLVSDEGIGELFVQYLCHDFEERSLPAEY
                     RPMREFVHAQSLDGVPRGMLARAFADARAAALQCDVDLRLPPLEERAKPTPLPRCDWP
                     WRGAYVSYRGDSMPCCMVSTPDRINFGNMLEQGVANVWRGAAYARFREQLANDDPSPI
                     CRSCSLYRGTF"
     gene            16377..17528
                     /locus_tag="VFO53_15300"
     CDS             16377..17528
                     /locus_tag="VFO53_15300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET8877695.1"
                     /translation="MSIIELATRRDLPRRVAVFRALQLGDLLCAVPALRAVRNGFPDA
                     EITLIGLPWASAFAHRFARLVDRFVEFPGFPGLPEREPDVAALPAFLADAQAARYDLA
                     IQMHGRGDLTNSIVGAFGARSMAGFCPPGGACPDPLRFLPWPESGTEIHRCIALTDFL
                     GLPRAGDRLEFPFGDEEIVQGEALVARHGLTPRRFVCVHPGARLYTRRWPSDHFAAVA
                     QDVVRRGFRVVLTGSRDEALITRRIARTLPSGSVVDLAGQTELGAFAALLRHARLLVA
                     NDTGVSHVAAALRVPSVIVCCGADPHRFAPLDHALHEVLHHPIACRPCTHVHCPIGHP
                     CAHKLTPDRVIARVGARLSQRNASPPLRFAVPLQPASRGQGTSPPPQGA"
     gene            17551..18495
                     /locus_tag="VFO53_15305"
     CDS             17551..18495
                     /locus_tag="VFO53_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET8877696.1"
                     /translation="MQRRLRILTWQVHGNYLYYLTHAPHEFFVLSKPGRPPGYAGRAG
                     TLPWGDNVHDCPVASVRGQAFDCVLFQSRVHFETDQHDILTDAQRKLPRIYLEHDPPL
                     AHPTSTVHPVDDPAVLIVHVTHYNALMWDCGRVPVRVVEHGVRVPDGIEWTGELPRGV
                     AVINHLERRGRRLGADVFAAWRREVPLDLFGMEAASSGGGCELPYAELLPAIARYRFF
                     AHPVRYTSLALALCEAMAIGLPVIGLATTELPRVIADCGGGYADTELARLLDASRELI
                     RDRGRAQELGRRARAYARERFAIGRFAADWDAVFREVT"
     gene            18526..19545
                     /locus_tag="VFO53_15310"
     CDS             18526..19545
                     /locus_tag="VFO53_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HET8877697.1"
                     /translation="MRTEPSQPLRVLVTGGAGFLGSHLCERLVDAGHDVLCVDNFYTG
                     TRANLASLLAHPRFELLRHDITFPLYVEVDRIFNLACPASPRHYQADPVQTTKTSVHG
                     AINLLGLAKRLRAPIVQASTSEVYGDPAQHPQTEGYWGHVNPIGPRACYDEGKRCAET
                     LFADYRRQHGLVTRIARIFNTYGPRMQPADGRVVSNFVVAALHGEPLTIHGDGWQTRS
                     FCFVSDTVDALLRLMDAPEPCAGPVNIGNPREFTVLELAERVRKLAGSRSSIVFIPAA
                     CDDPRQRRPDIALATRLLGWTPRVDIDEGLARTIDYFRRTLVPAARLRAKGEAPTGTH
                     ASGTR"
     gene            19784..20161
                     /locus_tag="VFO53_15315"
     CDS             19784..20161
                     /locus_tag="VFO53_15315"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET8877698.1"
                     /translation="MTAGTRLSLAFANIKALIQDQALLAVLEVQRAGVSLVKIIIAGI
                     VISILIVSAWMGIVAAAVGWAVGAGANWALAILVAALVNVAVAVALGFWAKKQVPDLL
                     FSATLRQLRKDVPRGENEYEPRR"
     gene            20145..20486
                     /locus_tag="VFO53_15320"
     CDS             20145..20486
                     /locus_tag="VFO53_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877699.1"
                     /translation="MSHDDKSLDEQIAEVEARLSRRRAELRLMAAEARNRISATKAIP
                     IAVVAALAVGFAASRFVRKPARPLPVEKRSRSMRMMGTVAGVMLPPLMRPLQHAAAQW
                     LAQRMQRAGAR"
     gene            complement(20529..20765)
                     /locus_tag="VFO53_15325"
     CDS             complement(20529..20765)
                     /locus_tag="VFO53_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877700.1"
                     /translation="MELWDLLQWPAMVATLASTWLVGSTRKSLRVVGFYTFLVSNALW
                     VAWGWHDHAWALVALQVGLAALNVRGVTKNETGA"
     gene            20964..21572
                     /locus_tag="VFO53_15330"
     CDS             20964..21572
                     /locus_tag="VFO53_15330"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET8877701.1"
                     /translation="MPTPSFLPSARVLVLACATLVLVGGCDRIELATFPGDSFRDRIA
                     LKATPAAYALAPVQRDFLTVAAQSNLFEIEASRVALARGGSAAVRRFAEATLRERVSV
                     DTDLEQLAQSVGVVLPSRLSDEFQARLAVLEQLTGTDFDRAYARNVGVLAQEQALAAF
                     ERAADPGGARVQRFAADQIPALRAHLEQGRRLASEVDRTNMA"
     gene            21643..22194
                     /locus_tag="VFO53_15335"
     CDS             21643..22194
                     /locus_tag="VFO53_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877702.1"
                     /translation="MSTATVMRALDALAPMLEALLADASISAEHAMHLVVMDPAAEVK
                     TTPFDDAILVERSFGDAASWQADYPWYAREKTRLAWREQMSLRTLFGQHPARLRDGDI
                     RVEGAVRSGRWVVGASGAQPWYDHAIATIAIALLEAAVESAHPPGEPNPVMAQGARDI
                     EQGKTDTDCYDAAGPRFDRKAPP"
     gene            22307..23710
                     /locus_tag="VFO53_15340"
     CDS             22307..23710
                     /locus_tag="VFO53_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8877703.1"
                     /translation="MQRNVLIPLIVACALFMENMDSTVIATSLPAIASDIGESPLALK
                     LALTSYLVSLAVFIPISGWVADRYGSRSVFATAIVVFIGGSLLCAASTTLEAFVISRF
                     LQGIGGAMMVPVGRLVLMRAIPKHDYVAALNYLTIPALLGPVTGPALGGAITLYFHWR
                     YIFIINIPIGILGLYLVLRHIPDIRETGMPAFDMRGFALSGVGLSVLMLGFSALGGHL
                     LPLPAAAACIAAGFVALVLYVRHARRTAHPVLDLDLLRLRTFWNGVVAGSVFRAGLGA
                     TPFLLPLLFQLGFGLDPLQSGLLTCMTAIGAMFIKTLTVFILRRFGFRTVLSVNGVAA
                     SLIVMAFGLFSSTTPHSVVAMVLLLSGCLRSLQFTALNAISFADIAPERMSQAASFSS
                     MAQRLSQSAGIAAGAYLLQLSSTVQGHASIEAADFWPAFLGIGLVSALAPLMHRRLAP
                     QAGIEVSGHGAPPRQRR"
     gene            complement(23734..>24930)
                     /locus_tag="VFO53_15345"
     CDS             complement(23734..>24930)
                     /locus_tag="VFO53_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET8877704.1"
                     /translation="AGRPEAIYIIERLIDEAARQTGIDRVELRRRNLIGPAQMPYTNA
                     MDKTYDSGQFAKVMDAALALCDWHGFAARAGDAKRRGRLRGQGIATFLEWTGADVFEE
                     TVDVTVTGAGRIEIFTAAQPMGQSLATTFTQLVVDVFGVPPELIDMRFGDTDRATGFG
                     SAGSRSLFVVGSAVRVASERTLSKSHALAAQQLEAAEADIEYRDGVFGIAGTDRRIGL
                     FELAQAQPGESIVVASTSSVGAASWPNGCHVCEVEVDPDTGEVELDAYWSVNDVGRVI
                     NPMVVIGQLEGGALQGIGQALCERVVYDAQSGQVLTASFMDYAMPRARMIGRFAMTMD
                     ESSPCLTNPMGVKGVGELGAIGATPAVVNAVADALARNGHASAVRELQMPLTPERVWR
                     AMQPRG"
BASE COUNT         3758 a         8819 c         8370 g         3983 t
ORIGIN      
        1 agccgcggga ctcacgcgct tcattctcgt cacgggccgc aacaagcgcg cgatcgagga
       61 ccatttcgac cgcgcttacg agctcgagca cgagcttgcc gcgcgcgaaa gcgacgtcgc
      121 gctgcgcgaa ctgcgctcgg cggttccaag cggcgtgacg ttcagctacg tgcgccagcg
      181 cgaggcgtcc gggcttcccg acgcgctgcg gagcgcccgt tccctcattg gtcaggcgcc
      241 gttcgcggtc atcctgcccg acgacctcat cgacgcgccg cgtccggcgc tggcacagct
      301 cctcgacgtg tacgacgagc agcaggtcag cgtagccggc gtgcagcgca gcgcacgcga
      361 ggcgagcgac aatggctgcc tcatcgcggc cgatctcaac accagcaagc tgcgcgcagc
      421 ccgcgagctc gtgaaggtgc cgccgatcgg cgccgcatcc gggccggttt cgctttgcgg
      481 acgctacgtc ttcacctccg cggtatggga cgcgatgcaa gacgagcgcg acccgatgct
      541 cgcgaaaacg gtgcgcgcgc tggtggagcg cgagcgcgtc tttgcgtgtc tcgtcgatgg
      601 gcagcgcttc gactgcggca cgaagctcgg atttctcgag gcgcaattcg cgtttgcgca
      661 gaagcacctc gaactgtggc cgggattgcg gcgcaggctc gaccatttgc tggcggacaa
      721 ggcggcgccg gtcgaagcgc cgaccgccgc atcatccacg ggcgccgtca aagccgcgat
      781 catgcgttga gcgccgtgcg cgtcgacgcc gcgagccgcg cgtcgaagtt acctgcaatt
      841 cctaccggcg gctcgggcgg ctgcatcgct tgcgggttga tttatcggca attgcgccga
      901 tggcatgtcg cttgcgatcc tgaacccgtc gctttcgact ggatcagccc atggcaggca
      961 acaccgaacc atccctcccc cgcgtgacga acgcaagcga tgcccacctg catcgcaatc
     1021 ccgacgaggg ccgtacgtgg ccgcgctgcg agccggcatt cacccatttc gagctcggcg
     1081 cgtcgtcgga acttcggcct accgccttga tggccaaacg ctacacgccg cgctacggcg
     1141 tacgaaagga atgacgatgg caaccacccg aacaggaaca ggcacgccga gcaccacgaa
     1201 cccgatgggt gtcggcgacg tcgacagccg ctaccgcacc ggcgacgact actggaccga
     1261 tcgcaacggc ggccccggac cgcaggtgat ggcggccgat acgctgcagg gcgactcggt
     1321 gcgcaacgac gcgggcgagg atctcggcga gatcaccgac atcatgctcg acgtgcccac
     1381 cggccgcgtt gcctacgcgg tgatgtcggt cggcggcgtg ctcggcatcg gtgacaagct
     1441 gttcgcgatc ccctggtccg cgcttcgcct cgataccgag aacaagtgct tccggatgaa
     1501 cgtcgcgaag gaacgcttcg agcaggcgcc gggattcgac aaggaccatt ggccgacgat
     1561 ggccgatcag cgctgggccg agaccgtgca ctcgttctac aacgcgcggc cgtactggtc
     1621 gtaatacgaa cggacgaaag tttcgcgcgc cttcatcggg cgcgcgatgc tttcgttcga
     1681 gtggtcttcc gcaaccggca cggggcgcga cgcacttcga gcggcactgg ggcacacgcg
     1741 tgcaattgga acgcgcatgc cgaacgcgcg tcatagtgag tccaatggtc acgcacacga
     1801 ccggacccgt tcgcaacgga aacggaaaca acggccatgg cggcaacgtc aaggccgacg
     1861 cacccgatcc cgggcttcgc gcaacgctga aaaaggcgtc gctgcgctac agccgctcga
     1921 ccgatcgcgg cttgcggcgc gaaggcacgc ccgaaacatt ccgctacgtc gacggcgacg
     1981 gccgcaaggt gcgggcgtcc gaaacgcttg cgcgcatccg catgctcgcg atcccgccgg
     2041 cgtggacgtc ggtatggatc tgcggcgacc cgcagggtca cctgcaggcg accggacgcg
     2101 atgcgcgcgg ccgcctgcaa taccgctacc acgcgcagtg gcgggcgcag cgcgacgagc
     2161 acaagttcga tcggcttgcc gaattcgccg cgtcgctgcc tgccatccgc gaagccgtcg
     2221 cgcgcgacct cgaacagccg ggattgccac gacgcaagct gctggccgcg atggttcgcc
     2281 tgctcgatcg cacctacgtt cgcgtcggcg acgaacgcta ccggcgcgac aacggcagct
     2341 tcgggctcac cacgcttcgc actcggcacg tgcaagttcg cggtgaccgc attcgcctgc
     2401 gctttcgcgg caaatcgggg aaggagcacg acctgtcgct tgccgaccgt cgtcttgcgc
     2461 gcatggtgcg ccgctgcctc gacctgccag gctacgagct cttccggtat gtcgaagacg
     2521 gcgaaacgcg gccgctttgc gcgaccgatg tgaatgagta cctgcaggag atcgccggcg
     2581 ccgattacac gtcgaaggat tttcgcacgt ggggcgcgac ggtgatcgca acggtgctcc
     2641 tcacgcgctt cggacttccc ggttcggtca gcgagcgcac gaagacggtg aaccgggcgc
     2701 ttcgcgccgc ggcgcaggcc ctcggcaaca cgctcgccgt gtgccgcaag agctacgtgc
     2761 atcccggtgt cgtcgaggcg tatctcggcg aggcgcttcc gccgcgccgc gtgcggatcg
     2821 aggcttcggg gctgcgcgcc ggcgaacggc gcacgcttgc gctgctcagg acgctgcgtg
     2881 ggccggtgaa gcgcgcgcgg catcgtcccc ggacacgaag cgcggacccg tccggcatcc
     2941 aggcggcgcc tccggcgccc tgaacaggcg cacccactgg cacttggtca ccggcgtgcg
     3001 cccggcaagc gcatcggcga tgaggcgttg ccagtggcgc agcacgcccg cgccgcgcac
     3061 gtcctgtgga tgcagcgaga ttcgcaacag cggcagcgca ttcgcccggc gcagctcgtg
     3121 cccgatcgcc gcccgcgaga cggcacgccg caacgcgctg tcggggctgt agcaaagatt
     3181 tgccgtgcgc tcgaagcgcc acgcaggcag atggtagatg ccgctgcgca ccgtcacgta
     3241 ctcgaacgca tgcgcgcaat gcgaaagcgc gctgcgtgcg ccgtcgccaa gcagccatgc
     3301 cggcggcgcg aagcccgcga gcggccagcc gagacggtcg aaaagcgcca ctccgcgcgc
     3361 gatgcgggac gccgcagctt cagcgtcgat cgctgcaaat tcgccttcgg tgcgcgtcag
     3421 gacgcgccgc gcaaaccatt gccgcagcgt gcgcggcgcc ggcgcgtcgt cgatgtggaa
     3481 atacccatgc agcacgagct cgtcgccgcg cgcgaggcgc gcatccattg cacgcacgaa
     3541 cgcatgctcg cgcaacacgc tcgtgcggtg atgatgccgt ggaatgacga gaagcgtgac
     3601 gggccccgcg ccggcattgt cgagcatcgc cagaagctcg cggcactcgc gccatgtcgc
     3661 aggcgaaaca tcgtggatcg cgatggccac cgcgcgttgc gcggggtcag ggcgtggcat
     3721 agcgcggcgt acgtgcggac gcggacgcaa tggcgccgcg atacaggccg aggagcccgc
     3781 gcatggcggt cgaccagctg tattcgctct cggcgcgggc gcgcgccgcg cggccgagcg
     3841 tatcgggatc gcgccggtag aatgcgcgca cgccttcggc cagcgccgcc gggtcgccgg
     3901 gtgtcaccag cacgccgtcg gccggcgtga cgatctcggg caacgcaccg gcgttcgccg
     3961 cgacgacgcc gcgtccgcac gccatcgcct cgagcacgac gagaccgaac gtttcctgat
     4021 cgccggcatg gacgaggccg tcgacgctcg cgagaatgcg tgcgagctcg agcgacgaat
     4081 gcagaaacgg cagcagcgtg acgttcgccg gcagcgcgag ctcgatctgc ggaccggcga
     4141 gcacgaggtg gtagtgcggg ccgagcgcgc gaaatgcatc gagcagcacc ggcaggttct
     4201 tttcggtgga gaagcggccg gcataggcaa gaaggcgcgc tcgcgcgggc agtccgagct
     4261 cgcgccgcaa gcccggtacg cgacggcgcg gcgagaatgt ggcgacatcg acgccgagcg
     4321 gccgcgcaac cacgcgctcg acaccccatg cattgagttg cgcacgcatg aattcgctcg
     4381 gtgctgcgac gagatcgcaa cgccgataga attcgcgcgc atacaactgg acggcggcgc
     4441 cggggagcgc accgagccgc cgggttgcca ttcgcgcgat gtcgctatga cagaacgcaa
     4501 tcagcggaac gtcaagcgag cgcgccgcgt gcagcgccgc ccagccttcg ggtcccgcat
     4561 ccccgacctc gatcaggtcg ggagcgagct cacgtatctg gcgcgaccag cgatgcgcag
     4621 cgaacggcca gcgatggccc tgacggccga gcgtgcgcgt gcgcaggaat atctcgccgc
     4681 tcgcaccccg cgcatcggcc gcgcccggca ccagcagcga gtgccggatc gacgtgtgcc
     4741 ggcgcagcca tgcgtgcttg gcgcgaagat agcgcaccac gccgccgctg gtcggcgcat
     4801 agagcatcgt gacgtcgagc aagtgcatcc gtcgagccgt tcctgccgtt tcgaacgaca
     4861 agcgccgccg gtcgcgcgcc cacgcgtccg acacaacccg tggaacggcg gccgccgccg
     4921 cggcggccgt gggcgtcatg catctccccg cgcactatct tgcgagcgtc gcaatctgtc
     4981 aaccccgcga acgatcgcta cttgcccttg ttcgcgtcgt tcggtgtggt cgacgtcggg
     5041 gcagacgagc tcgagctcga cgacgagctc gtgtcgggcg acgcgggcgg cgtgctcgcg
     5101 ctcgacgacg atgtgctggg agtcgacgac gggctcgatg acatcgatcc gctcgacgac
     5161 gaaggcggcg tcgtcgacga ggaagacgat gtcgagggcg acggcgtcac gacgggcggt
     5221 gtcgtgccgg gcgtcgtcga cgattcgcga ttgcaacccg cgatgccgag cacgagcccg
     5281 gtgcaaagca gtgtgaccaa tgcgctcttc attgcgatct ccagatgatt gaaagggggt
     5341 gtcccctgcg ggatttagca gattcggtgc catgcgaccg gcgccggatc gccgaagctg
     5401 cgcaaaaaat gacgaatcaa atcagcgctg tcgtcgatcg gatacacgca ttttcgacgc
     5461 gaaaagtgcg cgtaccgccc acgttcggcg cgcaaatttc atcggcggca ggtaataatt
     5521 acgcgcggtt tggaccgatc cgcggcacaa aagtgtttac cgtgcagatt catgcacatg
     5581 ggctgctcgc cgcaaccggt atgctccttg cgcggtattc ccgacacccg tcttcggccc
     5641 caccctcgca atgcctcatg ccctgatcgt cgacgatgac acgcatgccg cggaggcgct
     5701 cgcggaactc gtcgcggaga acggatttac aaccgcctgt gccgattcgc tgcaaggtgc
     5761 acgcgatctg ctttcgacgt cgcccgacgt gatcctgctc gacctgatcc tgcccgacgg
     5821 caacggtctc gagctgttgc gcgacgcgac gacgcgcgaa agtgaaacgc aggtcgtcgt
     5881 gatcaccggt cacgcgacgc tcgaaagctc gatcgaggcg atgcgccacg gcgcgaccga
     5941 ctacctgctg aagcccatca acctggcgca gctgcgcggc gtgctgtcgc gtgtcgcccg
     6001 gccgcccgag ctttcggccg aggtcaagga cctgcgcacc gagttgcgct cgctcggcca
     6061 cttcggccgc ctcgtcggcg cttcggcgcc gatgcagcgg ctgtacgacc agctctcgcg
     6121 cgtggcgccg accaacgcca cggtgttcat caccggcgag agcggcacgg gcaaggagat
     6181 ggtcgcgcag acgatccacg aggtgagccg gcggaggctc cgcccgttcc tcgccgtcaa
     6241 ttgcggcgcc atttcgcccc agatcatcga aagcgagctt ttcggccacg agaagggaag
     6301 cttcaccggc gcgaatcgac agcaccgcgg ttacttcgag cgcgcgcacg gcggcacgct
     6361 gttcctcgac gagatcaccg aaatgccgat ggacctgcag gtgaagcttt tgcgcgtgct
     6421 cgaaacgcag acggtgagcc gcgtggggtc cgatcgcctg atcgagatcg acgtgcgcgt
     6481 gcttgccgcg agcaatcgca atccgcatga cgcggtcgcc gccgggaagc tgcgcaagga
     6541 ccttctgtac cggctgcagg tgtttccgct ttacgttccg ccgctgcgcg agcacagcga
     6601 cgacatcgag ctgctcgccg cgcactttct cgccgacctg aaccggcgca gcaacacgaa
     6661 caaggcgttc acgccggccg caatcgatcg cctcaagcgc taccactggc cggggaacgt
     6721 gcgcgagctg tcgaacgccg tgcaccgcgc ctacatcatg accgacggcg actggatcac
     6781 ccaggtggga ctcgctcccg agccgacact gtccgccgat ttcagcggcg gccgctcgtt
     6841 ccaggtgggc gtcggcgatc gcatcgacgc gatggagaag cggctgatcc tcgcgaccgt
     6901 gcagcacacg catacgaagg aagcggccgc ggagattctc ggcatcagcg tcaagacgct
     6961 gtacaaccgc ctgcgagcct atgaatcggg cgccgacctt cgcgccgatg cagcgggcga
     7021 cgcgccggcc gcggcgtcgc cggcctcgcc ggggtcgcgt acaccggcca catgagcgat
     7081 cgtgctgacg agcaggtcga gatcgaccgg cttcgcgacc accgcatcga agcccgcatc
     7141 gatcgcgcgg gcgcggtcct cggcgcgggc gtagcccgtg cacgcaatgg ccggcgtgct
     7201 tccgccttcc tccgcgggca ggtggcgcac gcgccggatg agctcgtatc cgctttcgcg
     7261 cggcatgccc acgtcggaaa tcatcaggtc ggggtgccac acgacgagcg tctgctgcgc
     7321 ctcgcgcgcg gaagccgatg cgcgcacgca ggcaccgcgc gtctcgagcg cctcggcgag
     7381 gagcgcgcgc gtggtggggt cgtcgtcgac gatgagtagc ttgaggttcg agcgcggcaa
     7441 cgtgtgcatg gcggcgggcc acccccttcg cggcgacaac ggaattcgat gaacgcccga
     7501 aacgtgttgc aggaatcggg cctgcccgac agccggctcc tcgcgcgcct gctgcaggct
     7561 ggcacgctgg tgctcgacga ggacgacacg ctgaccttcg caagcgctgg cgcatgcgac
     7621 ctgctcggcg tcgatgacga ggccgcgctg cgcgagcact ggagcgagct tcgcgcgcag
     7681 ttgcgcgtcg accgatggcc gcgcacgctg cccgacggcg acgcctaccg cggccgtgcc
     7741 gatgtgctga ccccggcggg accgcgcgcg atacgcttcg aaatgcacgg catggccgac
     7801 gcgggatcgc tgcatcgcgc cgtcctcgtg cgcgatcgcg cgcacgttct cgcaacggac
     7861 agcgcgctgt tgcttgcgag cgaggcgcag gcgaaccggc acgtgctcac cgggctcgtg
     7921 catgcggcaa agggaccgct caacaatttc aacctgacgc tcgccctgct gtccgcgggt
     7981 attgcgcgcg gcgacgcgct tgcggcgacg cccgaggcgg ccgcgcggcg cacgcgctac
     8041 atcgacgtgc tgcagaagga agccacgcgt ctcgcgtcgt gcatcgacga gatccacgcg
     8101 ctgaccgttc gccacgagcc gtcatgggag ccgatcgatc tcgccgcgac gtcgatcgat
     8161 tgcgcacgcg tgctgcggca cggcgccacg atgcgcgagg tgagcctcga gctcgccgtg
     8221 cccggccagc ctgtcatggc aatcggcgat ccgcaactcg tgcgcctcgc gctgctgtcg
     8281 ttcacgatct gcgtgctcga gctcacaccc gcaggaggcc gcgtcgcttg gcgcgtgatg
     8341 cacgaccgcg acacgcatgc gccgtcggtc gccatcacca cgtcgcagcc cgtcctgcca
     8401 cgcgcgctcg ccaccgcgct gttccggctg tcctgcatgg cggaatccga ttactccggt
     8461 gcaatcgccg cgcgcctcat cgtcgaggcg cagggaggcg aggtgatcct gcacgacgcc
     8521 ggcgacggcg cagcgggcat cctgctgcac atccccgggc acgcctgaag cgcgcgcgat
     8581 cagcgccggc aacgacgcgc cggcggtgca gtcgttcact ggttgccggt aagaattgcc
     8641 atcccccgcg ctgcggggtg cgcgcttctc gtggcgctcc gtcttgaagc gacgctcgag
     8701 cgggaagcgg cacgcggctt gcagaaactg cgacatgcaa acgcgatcga tcaatgcgct
     8761 tgccgcctta tgcaacaacc atgacgccga cccggtgaca gcctgcatag cgatgatcag
     8821 tgagcatgcg tcgccgctcg caacgatcgg cggcgtcgac gcgggcggac agaacatcta
     8881 cgtggcgcac accgcccggc acctcgcggc tctcggctac gcggtggaca tcttcacgcg
     8941 cagggactcg gaggcgcttc ccgacgtcgt cgattggatg cccggcatac gcgtcgtgca
     9001 cgtgcgtgcc ggccctgcgt cgttcgtgcg caaggaatcg ctgctgccgc acatggcgca
     9061 gttcgcgcgc agcgtcgtcg accacgcgaa gtccgcgcgc gatcgcggcg cgccgtatgc
     9121 ggtttgtcat gcgcacttct tcatgtcggg cctcgtcgca cgccggttga agcaggcgct
     9181 tggcatcccg tacgtcgtca cgtttcacgc actcggccgc gtccggctgc tgcaccagcc
     9241 gcacgacgaa tttccgccgc agcgctgcat cctcgagcag atggtgatcg acgaagcgga
     9301 tgcgatcgtt gccgaatgtc cgcaggatga aatggacctg cagcgccact accgcacgcc
     9361 ggcttcccgg atgcgcatga ttccctgcgg attcgatccc gctgaactgc agccggtggc
     9421 ccgcgcgaag gcgcgtgcgc atctcgggct gcctggcgag cgcccgatcg tgctgcagct
     9481 cgggcgcatg gttccgcgca aaggtgtcga cacggtgatc cacgcgctcg gcgtgctcga
     9541 gcgccgctac cgcttgaacc cgctgctgct catcgtcggc ggcgaatcgc cgacccccga
     9601 tccggtgcgc acgccggaga tcggccggct gcagcgcatc gcgcaggacg agggcgtcgc
     9661 cgccgacgtc cgattcgtcg gccagcgtcc gcgctgcgag ctgcgctatt tctacagcgc
     9721 ctccgacgtc ttcgtcacga tgccgtggta cgagccgttc ggcatcacgc ccgtcgaggc
     9781 gatggcgtgc ggcgttccgg tcatcggtgc gcgcgtcggc ggcgtccagt actcggtcga
     9841 ggatggccgc accggttttc tcgtcgaagc gaaaaacgcc gacgcgctcg cgcgccgcct
     9901 cgcgcacgtg ttcagcgacg cgtcgatccc gcgcctgctc ggcaagcgcg cgcggcgccg
     9961 cgcgtgggaa cgcttcacct gggaacggat ctcgcgttcg ctcgccgacc tctacgccga
    10021 ggtcgcgacg gccgcttcgc tgccgcgccc cgcactcgcg caaggcacgc gctagaagga
    10081 tcgtttggat accgatctca cgcagaaggt cgtcgtcgtc accggcggcg gccagggtct
    10141 gggggctgcg atctgcagcg agcttgggca ggccggcgcc aacgtgttcg tcgccgatcg
    10201 cagcgaacga gccgaaggcg tggcggcggc actcacccgc gacggccagc gggcctatgc
    10261 gctgcggggc gacgtcggca gccccgacga cgtaagacgc ttcgtcgacg aggcgaagaa
    10321 gcgcttcggc cggctcgacg cggtgatcaa caacgcgggc atcgatcgca cgaccggcgt
    10381 cgacgagctc gacgtcgacg actgggacgc catcatccgc accaatctgc gcgggccgtt
    10441 cctgctgtcg aagttcgcgc tgccgctgct gcgcgacagc cagggcgaca tcgtcaacgt
    10501 cgcgtccacc gcggcgaagc gcggctggcc gaatgcgagc gcgtatcacg cgagcaaatg
    10561 gggcttgctc gggctgtcgt acgcgatgca cgccgagttc cgcgcgcaca aggttcgcgt
    10621 caccgccgtg atcgccggcg gcatgcgcac gccgttcctg ctcgagcgat ttcccgacct
    10681 cgaccccgcc aagctccagg atccgcgcga cgtcgcgcgc gcggtgcgct ttgcgctcag
    10741 ccagccgcgc gagtcggtcg tcgccgaaat caccgtgctt cccgtggagg aatcgtcatg
    10801 gccatgaacg gcgcatcgca ccagccgcaa gcgtgggacg tcggacccgc ggccgcggtg
    10861 cgcgatgcca cggcgatctt catcgacaag gatggcacgc tcgttcacga cgtgccctac
    10921 aacgtcgatc cggcacgcat cgagctcgtt cccggtgccg gcgacgcgct gcgcgcgctg
    10981 caggatgcgg gctttctgct tttcgtcgtc tcgaaccagc ctggcgtggc gcttcggcgc
    11041 tttccgatgc gcgccctgcg caccgtcgag gcgcgtctcg acgaactgct cgcgccgttc
    11101 ggcgtcatgt tcaccgcgta ttgctggtgt ccgcatccgc cggccggcgt gcgcgccgcc
    11161 atcgcatgca cctgccgcaa gccgcgcccc ggattgctgc tcgacgcagg cgctacgcat
    11221 ggcgtggatc tcgagcgctc gtggatgatc ggcgacattc tcgatgacat cgaggccggc
    11281 aatcgcgcgg gatgccgcac cgtgctcgtc gatcgcggca acgagacgcg ctggcgcacc
    11341 gggcgcctgc gcacgcccaa cgcgatcgtc tatcagctcg cggacgccgc cgagatcatt
    11401 ctcgatggcg cgcgagacca atcggccgcg cgtccccgcg cggcgccggc atgatcacga
    11461 tggacccttc gtggcgcagc gcgcgctcgc tcctcgccgt gcgcctcgac ggcatcggcg
    11521 acctcctgat gaccacgccg gcgctgcgcg cgcttaagca ggaccgccgc ggccgttcgc
    11581 tgacgctgct gacgtcgccc gccggcgcca tcgcggcgcg cgagctcccg ttcgtcgacg
    11641 atgtgatcgt gttcacggcg ccgtggatga aggcggccga ggccgtgccc gtgtcgcccg
    11701 tcgccaccgc gagcctcgtc gagcagctgc gcgagcgtgc attcgacgcc gccatcgtgt
    11761 tcaccgtctt cacgcaaagc gcgctgcctt cggcgacgct gctgcatctc gccggcatcc
    11821 cgcgccgcgc cgcgtacagc cgcgagaacc cgtatgcgct gctgacagac tggcgtgccg
    11881 atcccgaccg cgacgcgcat gccggcgtgc gccacgaggt gcagcgccag atcgatctcg
    11941 tgcaaagcct cggcacgcgc gttcgcgaca cccgtctgtc gtttccggtg cgcgaagacg
    12001 cgcggcgcag catgctcgcc tgcgtcgtcg ccgccgggat ggagcccggc cggccatggc
    12061 tcatcgtcca tccgggcgcc accgcggcgt cgcggcgcta tcctcacgac aagctcgccg
    12121 acgcgatcgg gcggctgcag cagcgcggcc gctggcagat cgcggtcgcc ggcacggcag
    12181 aggatcgcga atgcgcacgg cgcatcgcgg cgagcgtgaa cggcgtcgtt ccgctgacgg
    12241 gcgtcctcga cctcgccgag ctcgccgcgc tgctgcaggc ggcggagctc gtcgtctgca
    12301 acaactcggc gcctgcgcac atcgccgccg ccgtaggcac gcccgtcgtc gacctgtacg
    12361 cgctgaccaa tccacagcac acgccgtggg gtgtcgcgca ccgcgtgctg tcgcacgacg
    12421 tcgattgcaa gtactgcctg aagagcgcat gcccgcagcg gcatcaccgc tgtctcgcgg
    12481 atgtcgcgcc cggcgagatc gtcgccgccg tcgatgcgct gcttcccgat gcgccggtcc
    12541 gcgtgccgcc gcgcgtgcgc gcgttcgccg gcgccggcgc cggcgccggc gcttgagcgt
    12601 tccgatgcgc acgctcggca tcaatgccgc cttccacgac tcggccgcgt gcctcgtcga
    12661 cgacggcgcc gttgtcgcag cggccgaaga agagcggttc acccagatca agcacggcaa
    12721 gcgcccggtt ccgttttccg cgtgggagct gccctttcac gcgatcgact actgcctcgc
    12781 cgaggcgggc atcgacctcg acgcgatcga tcacgtcgcc tattcgttcg aacccgggct
    12841 tttcgtcaac ggcagcggaa tgccgtcgca gatcacgctg ccgctcgagc cgagtgccat
    12901 ccgcgcgccc gccggcaagg cgccgtggga cccgcttttc ctgtcgtacg tcgtgaacgc
    12961 gccacgccag ctgtccggtg gcgcaccgca ccacctgcag cagcggctgc gctcgcgcgt
    13021 caacgcgccg cactggcaat ggcattacgt cccgcatcat ctggcgcatc aggcgagcgc
    13081 gttcctcgcc gcgcccttcg acgagtgcgc cgtgctgacg ctcgacggcc gcggcgagaa
    13141 cacgtcgacg caatacggag ccttccgcga cggccgctac acgcgcattt cggaggtacc
    13201 gctgccgcac tcgctcggcc ttctgtacga agacgtcacg cgccacctcg gcttcctgca
    13261 ttcttccgac gaatacaagg tcatggcgct ggcgtcgttc ggcacaccgc gcttcgtcga
    13321 tgcgctgcgc gagcacgtac gctggcaggg cgatggccgt tacgtcgtcg atgcgccacc
    13381 gtttgcaagt ttctgcgcgc cacgcaagcc gggcgagccg ttcctgcccg tgcatgccga
    13441 cgttgcgcac gcgacgcagc gcgtgctcga ggaaacggtg ctcgggatcg tgcggtggct
    13501 gcgcgccacc acgcaactgc cgaacctcgc catggcgggc ggcgtggcgc tcaactgcgt
    13561 catgaacgca gccctgcgcg atgccgcggt cttcgaccgg atctgggtgc agcccgccgc
    13621 cggcgacgcc ggcacggcgc tcggcgcggc gctgtggatc gatcgcgaag cgcgcggcgg
    13681 cgacgccgcg aagcgcgcat tccgcatgga ccacgcctat ctcggtcccg ggtactcgga
    13741 cgccgagatc gagaccgcgt tgcggcatgc gcgcgcgcgc tacgaggcgc ccgcggacct
    13801 ccccgggacg attgccgcgc agctcgaacg ggaccgcatc gtcggctggt ttcagggccg
    13861 gatggaattc gggccgcgcg cgctcggcgc gcgctcgatc ctcgcgtcgc ctctgcatgc
    13921 gtcgatgcag gcgcgcttga acgagctcaa ggatcgcgag gatttccgcc ccgtggcgcc
    13981 cgcggtcctc gaggaagcgg ccagcgactg gttcgaaaac tgtacgagct cgccgttcat
    14041 gctgttcgtg caccgcgttc cgccggcgcg ccgcgcgcgc attcccgctg cgtgccacgc
    14101 cgacggcacg gcgcgcgtgc agacggtgtc gcgcgacgcg aacccgctct accacgcgct
    14161 gatcgggcaa ttcgccaggc gcaccggcgt tccggtcgtc atcaacacgt cgttcaacac
    14221 gcgcggcaag ccgatcgtct gctcgccgcg cgacgcgctc gagtgcttct tcacctcgcc
    14281 gctcgacgtg ctcgccatcg gtcgctacgt catcgaaaag ggggcgcatt gaaccccgag
    14341 gtctccgtcg tcatcgccac ctacgagcgc cgcgacctgc tgctgcgctg tgccgatgcg
    14401 ctgctcgcgc agacgcttgc ggccgcgcgc ttcgagatcg tcatcgtcga cgacggctcg
    14461 tcgcgtgcca cgcgcgaata cgtgatggcc gagctttcgt cgcggcgccg gctgcggcgc
    14521 gcaccgacgc tgcgcttcct gtggctgcct tcgaatcgcg gacccgccgc ggcgcgcaac
    14581 tgcggcattc tcgcggcgcg cggcggcgtg atcgcattca ccgacgacga cacggtgccg
    14641 cacgcgaact ggctcgaatc ggggctcgat gcgatcaggc atggcgccga cgccgtggcc
    14701 ggcgcggtcg aggtgccgct tcccgcggta ccgaccgatt acgagcgtga cgccgcgggc
    14761 ctgtcgaacg ccggcttcgt cacggcgaac tgcttcgtgc ggcgcgacct gctttgtgcc
    14821 gtgggtggct tcgacgagcg cttccgctgc gcgtggcgcg aagataccga cctcttcttc
    14881 cggttgcgcg caagcggcgc gatcgtgcgc gcgtcgcatg ccgcacgcgt cgtgcatccg
    14941 gtgcggccgg cgcgctgggg cgtgagcctc gcgcagcagc gcaaggtgat gttcgacgcg
    15001 ctgctgtaca agaaattccc gcagggctac cgccgctacg tgcgtgcacg gccgccgtgg
    15061 gactactact gcgcggtcgt ggcgggcctt tgcgccctgg tcaatgccgt ggccggcttc
    15121 tggctgctcg cacttgcgtg ccttgttgcg tggctcggca tcagcgtggc gttcgcgtcg
    15181 cgccggctcg ccgacacgag ccacgcgccc cggcacgttg ccgagatgat cgtcacgtcg
    15241 ctgctgatcc cgtgggtctc ggtgtactgg cgaacgcgcg gcgcgctgcg ctttcgcgtt
    15301 ccgttcgtat gatccgcaag gtcgcctcgt gacgcccctc gatgccgtgc tcgcaccggc
    15361 cgttgcctgg ccacgcacgc cgccgctgcc acgcttcgtg cagatcgagc cggtgggcca
    15421 gtgcaacctg cgctgccaga tgtgcccgat ccagttccgc aaggacgggc cgccgcacgg
    15481 tccgctcgcg tttctcgcct tcgacgactt cacgcggctc gtcgagggat tcggcgacat
    15541 cgacgagctg cagctgcagg gcctcggcga gccgacgatg catccgcaat tcttcgcgat
    15601 ggtcgcctgg gcgagcgcac gcggcattcg cgtctccacg aacagcaacc tcacgctgtg
    15661 gtccgagcgg cgggcgcgcc agtgcgtcga cagcggactc gcaacgctgc acgtgagcct
    15721 cgacgcggcg acgcccgagc tctacgagcg cattcgttgc ggcgcccatt tcccgaaggt
    15781 gatccgcaac ctgcgccgcg tgatggctgc ccggcgtgcc cgccaatcgc cgctgcacgt
    15841 gcgcgtcgtg atggtcctga tgcgccagaa cctgcacgag ctgcccgcgc tggtgcgcct
    15901 cgtgagcgac gaaggcatcg gcgagctgtt cgtccagtat ctctgtcacg acttcgagga
    15961 gcgctcgctg cccgccgagt accggccgat gcgcgagttc gtccacgcgc agtcgctcga
    16021 cggcgttccg cgcgggatgc tcgcgcgggc atttgccgac gcgcgcgccg ccgccttgca
    16081 atgcgatgtc gacctgcggc tgccgccgct cgaggagcgg gcgaagccga cgccgctgcc
    16141 tcgctgcgac tggccctggc gcggggcgta cgtgagctat cgcggcgatt cgatgccctg
    16201 ctgcatggtg agcacgccgg atcgaatcaa tttcggcaac atgctcgagc agggtgtggc
    16261 gaacgtctgg cgcggcgccg cctacgcgcg cttccgtgag cagcttgcga acgacgaccc
    16321 ctcgccgatc tgccgctcct gttcgctgta ccgcggtacc ttctagccgg ggcggaatga
    16381 gcatcatcga actcgccacc cgccgcgatc tgccgcgcag ggtcgccgtg ttccgggcgc
    16441 tgcaactcgg cgatctgctg tgcgccgtcc ccgcgctgcg cgcggtgcga aacggcttcc
    16501 ccgacgccga gatcacgctg atcggcctgc cgtgggcaag cgcattcgcg catcgcttcg
    16561 cgcggctcgt cgatcgcttc gtcgaatttc ccggctttcc cgggttgccg gagcgcgagc
    16621 ccgacgtggc cgcgctaccg gcctttctgg ccgacgcgca ggcggcacgc tacgacctcg
    16681 ccatccagat gcacggccgc ggcgatctca ccaattcgat cgtcggcgcc ttcggcgcca
    16741 ggtcgatggc cgggttctgc ccgccgggag gtgcgtgccc ggatccgctg cgcttcctgc
    16801 cgtggccgga aagcggcacc gagatccacc gctgcatcgc gctcaccgac ttcctcgggc
    16861 ttccgcgcgc cggcgaccgg ctcgagttcc cgttcggcga cgaggaaatc gtgcagggcg
    16921 aggcgctggt tgcgcggcac gggctcacgc cgcgccgttt cgtctgcgtt caccccggtg
    16981 cgcgcctcta cacgcggcgc tggccgtcgg accatttcgc ggccgtcgcg caggacgtgg
    17041 tgcgccgcgg ctttcgcgtg gtgctgacgg gcagccgcga cgaggcgctc atcacgcggc
    17101 gcatcgcacg cacgctgccg tccgggagcg tcgtggatct cgccggccag accgaactcg
    17161 gcgcattcgc ggcactgctt cgtcatgcac ggctgctggt ggcgaacgac accggcgttt
    17221 cgcacgtcgc cgccgcactt cgcgtgccga gcgtcatcgt ctgctgcggg gccgatccgc
    17281 accgcttcgc accgctcgac cacgcgctgc acgaggtgct ccatcacccg attgcgtgcc
    17341 ggccgtgcac gcacgtgcac tgcccgatcg gccacccatg cgcgcacaag ctcacgcccg
    17401 accgcgtcat cgcgcgcgtc ggtgcgcgcc tttcgcagcg caatgcgtcg ccgccgctgc
    17461 gctttgcggt gccgttgcag ccggcgagcc gcggccaagg cacgtcgccg ccgccgcaag
    17521 gcgcatgagc cggctgcgga cgaaccagcc atgcaaaggc gcctgcgcat cctgacatgg
    17581 caggttcacg gcaactacct ttactacctg acgcacgcgc cgcacgaatt cttcgtgttg
    17641 tcgaagccgg gtcgtccgcc cgggtacgcc ggccgcgcgg gaacgctgcc atggggcgac
    17701 aacgtgcacg attgcccggt cgcgtcggtg cgcggacagg cgttcgactg cgtgctcttc
    17761 cagtcgcgcg tgcacttcga aacggatcag cacgacatcc tgaccgatgc ccagcgcaag
    17821 ctgccgcgca tctatctcga gcacgatccg ccgctcgcgc atccgacgag caccgtgcat
    17881 ccggtcgacg atccggcggt gctgatcgtg cacgtaacgc actacaacgc gctgatgtgg
    17941 gattgcggcc gcgtcccggt gcgtgtcgtc gagcacggcg tccgcgtgcc ggacggcatc
    18001 gagtggaccg gcgagctgcc gcgcggcgtc gctgtcatca atcacctcga gcgccgcggg
    18061 aggcgtctcg gggccgacgt tttcgccgcg tggcggcgcg aggtgccgct cgatctcttc
    18121 ggcatggagg ccgcgtcgag cggcggcggg tgcgagctgc cgtacgccga gctcttgccg
    18181 gcgatcgcgc gctaccgctt cttcgcgcat ccggtgcgct acacgagcct cgccctcgcg
    18241 ttgtgcgagg cgatggcgat cgggttgccg gtgatcggtc tcgcgacgac cgagctgccg
    18301 cgcgtgatcg cggactgcgg cggcggctac gccgacaccg agcttgcgcg ccttctcgac
    18361 gcttcccgcg agctcattcg cgatcgcgga cgcgcgcagg agctcggccg gcgggcgcgg
    18421 gcgtatgcac gcgagcgctt cgcgatcggg cgctttgccg ccgactggga cgccgtgttc
    18481 cgcgaggtga catgagcggg atcgacactt gatggggacc gatcgatgag aaccgagcca
    18541 agtcagccgc tgcgcgtgct cgtcaccggg ggcgccggat tcctcggctc gcatctttgc
    18601 gagcgtctcg tcgacgccgg gcacgacgtg ctgtgcgtcg acaatttcta cacgggtacg
    18661 cgcgccaacc tcgcgtcgct gctcgcgcac ccgcgcttcg agctgctgcg ccacgacatc
    18721 acctttccgc tttacgtcga agtcgaccgc atcttcaacc tcgcgtgtcc ggcgtcgccg
    18781 cggcactacc aggccgatcc ggtgcagacg acgaagacga gcgtgcatgg cgcgatcaac
    18841 ctgctcggcc ttgccaagcg gttgcgcgcg ccgatcgtgc aggcgtcgac gagcgaggtc
    18901 tacggcgatc cggcgcagca tccgcagacc gagggctact ggggccacgt gaatccgatc
    18961 ggcccgcgcg cctgctacga cgaaggcaag cgctgcgccg aaacgctttt cgccgattac
    19021 cgccggcagc atggcctcgt cacgcgcatc gcccgcatct tcaacacgta cggtccgcgc
    19081 atgcagcccg cggacgggcg cgtcgtgtcg aacttcgtcg tcgccgccct gcacggcgag
    19141 ccgctgacga tccacggcga cggctggcag acgcgctcgt tctgcttcgt gagcgacacg
    19201 gtcgacgcgc tgctgcgcct catggatgca cccgagccgt gcgcgggccc ggtcaacatc
    19261 ggcaatcccc gcgaattcac cgtgctcgag ctcgccgaac gggtgcgcaa gctcgcagga
    19321 tcccgctcgt ctatcgtgtt cattccggcc gcgtgcgacg acccgcgcca gcgcaggccc
    19381 gatatcgcgc tcgcgacccg gctgctcggc tggacaccga gggtcgacat cgacgagggg
    19441 cttgcgcgca cgatcgacta ctttcggcgc acgctcgtgc cggccgccag gctgcgcgcg
    19501 aaaggcgaag cgcccaccgg tacgcatgcg tccggcacca ggtgaccggc gcgccacgca
    19561 aagaatacgc agcgtgcgcg cggccttggt tacgagcggc gcggtgtaag agttgccggt
    19621 gcgccggcga tcgcttcagg cgaatggcat gccggttgca tcagcgggta tgatcgacgg
    19681 gcttcgcgtc cgggcgatgg cggggttgcc gctcgcgccg cgcaacgaaa gacggatgcc
    19741 gaccatgaat cccacgtacg cgccaccgca tcgacccacg cccatgactg ccggcacgcg
    19801 cctctccctc gcttttgcga acatcaaggc gctcatccag gatcaggcgc ttctcgcggt
    19861 cctcgaggtg cagcgcgccg gcgtttcgct cgtcaagatc atcatcgccg gcatcgtgat
    19921 ctccatcctc atcgtgtccg cgtggatggg catcgttgcc gctgcggtcg gctgggcagt
    19981 cggcgcgggg gccaattggg cgctcgcgat cctggtcgca gcgctcgtca atgtcgcggt
    20041 tgcggttgcc ctcgggttct gggcgaaaaa acaggtgccc gacctgctct tttcggcgac
    20101 gctgcgccaa ctgcgcaagg acgtgccgcg cggggaaaac gagtatgagc cacgacgata
    20161 aatcgctcga cgagcagatc gccgaagtcg aggcgcggct ctcgcgtcgt cgcgccgagc
    20221 tgcgcttgat ggcggccgaa gcgcgcaacc ggatcagcgc caccaaagcg ataccgatcg
    20281 ccgtcgttgc cgcgctggcg gtcggcttcg ccgcctcgcg tttcgtgcgc aagccggcgc
    20341 ggccgctccc ggtcgagaag cgctcccggt cgatgcgaat gatggggacg gtggcgggcg
    20401 tcatgctgcc gccgctgatg cgcccgctgc agcacgcggc ggcgcaatgg cttgcgcagc
    20461 gcatgcagcg cgctggggcg cgctgaccac gtgcaaccgc gcgtcttgcg cgcgccgaag
    20521 cgacgcaatc aggcgcccgt ctcgttcttc gtgacgccgc gcacgttgag cgcggcaagg
    20581 ccgacctgca acgcgaccag cgcccacgcg tgatcgtgcc agccccaggc gacccacagc
    20641 gcgttgctca cgagaaacgt ataaaagccg accacacgca acgactttcg ggtcgagccg
    20701 acgagccagg tcgacgcaag tgttgcgacc atggcgggcc attgcagcag atcccagagc
    20761 tccatgatgt tttgcgcggc ccggtcagtg ggcggtgcgc ggccaacaac gtaattctta
    20821 ccagatcgct gccgtaacag ttgccgccgt tcggccgacg cctgcgcgcg acgcgcacgt
    20881 cgcgcacgag cgtccacgct catgacgccg gtctagcagc aggcaagcaa aatgcgcggc
    20941 atgccgcttg ctaaaattgc atcgtgccga cgccatcgtt tcttccctcc gcgcgcgtgc
    21001 tggtcctcgc gtgcgccacg ctcgtgctcg tcggcggctg cgaccggatc gaactcgcga
    21061 cgtttcccgg cgattcgttc cgcgatcgca tcgccttgaa agcgacaccg gctgcctacg
    21121 cgctcgcccc ggtccaacgc gatttcctga cggtggcagc gcaatcgaac ctgttcgaga
    21181 tcgaggcgag ccgcgtggcg ctcgcgcgtg gcggctccgc cgcggtgcgc cggtttgccg
    21241 aggcgacatt gcgcgagcgc gtatcggtcg ataccgatct cgagcagctt gcgcaaagcg
    21301 tcggtgtggt gctgccctct cggctctccg atgagttcca ggcgcgactc gccgtgctgg
    21361 agcagttgac cggaaccgac ttcgatcgcg cttatgcgcg aaacgtcggc gtgctcgccc
    21421 aggagcaggc actagccgcg ttcgagcgcg ctgccgatcc cggcggagcg cgggtccagc
    21481 gctttgcggc cgaccagatc ccggcgctgc gtgcgcatct cgagcagggg cgccgcctcg
    21541 cgtccgaggt cgaccgcacg aacatggcct gacgtccccg tgcgtgcaga aaccgccggg
    21601 gccgccggcg cagggcagca cgcgcacgcg gctgcgcacg ccgtgtcgac cgccaccgtc
    21661 atgcgcgcgc tcgacgcgct cgctccgatg ctcgaagcgc tcctcgccga tgcgtcgatc
    21721 agcgccgagc acgccatgca cctcgtcgtg atggatcccg ccgccgaggt gaagacaacg
    21781 ccgttcgacg atgcgatcct cgtggagcgc agcttcggcg atgctgcgag ctggcaggcc
    21841 gactatccgt ggtacgcgcg cgagaagacg cggctcgcgt ggcgcgagca gatgagcctg
    21901 cgcacgctgt tcggccagca tccggcgcgc ttgcgcgatg gcgacattcg cgtcgagggc
    21961 gctgtgcgaa gtggacgctg ggtcgtcggc gcaagcggcg cgcagccgtg gtacgaccac
    22021 gcgatcgcga cgatcgcaat cgcgctgctc gaggcggccg tcgagagcgc gcatccgccc
    22081 ggcgagccga atcccgtgat ggcgcagggc gcacgcgaca tcgaacaagg gaagaccgac
    22141 accgattgct atgatgcggc cgggccgcgc ttcgaccgca aggcgccgcc gtagcgattg
    22201 cacacgcacg ggcaacgcgg gggcgaccac gcgccgatgc gaattcgaag aatcgaagtg
    22261 gggaccgacg ctctcggtcc ctcttttttg ccgcgcttca cgcccattgc aacgcaacgt
    22321 cctcatcccc ctcatcgtcg cgtgcgcgct gttcatggag aacatggact ccaccgtgat
    22381 cgcgacgtcg ctgccggcga tcgcgagcga catcggcgag agcccgctcg cgctgaagct
    22441 cgcgctgacg tcgtatctcg tgagcctcgc ggtgttcatc ccgatcagcg ggtgggttgc
    22501 cgaccgctac ggctcgcgca gcgtctttgc cacggcgatc gtcgtgttca tcggcgggtc
    22561 gctgctttgc gcggcgtcga cgacgctgga ggcgttcgtg atctcgcgct tcctgcaggg
    22621 catcggcgga gcgatgatgg tgccggtagg ccggctcgtg ctgatgcgcg caatccccaa
    22681 gcacgactac gtggctgcgc tcaattacct gacgattccg gcactgctcg gaccggtcac
    22741 cggtcccgca ctcggcggcg ccatcacgct gtacttccat tggcgctaca tcttcatcat
    22801 caacattccg atcggcattc tcgggctgta cctcgtcctt cgccatatcc ccgacatccg
    22861 cgagaccggg atgccggcct tcgacatgcg cggcttcgcg ctgtcgggtg tcggtctgtc
    22921 ggtcctcatg ctgggcttct cggcactggg cgggcacctg ctgccgctgc ccgccgcggc
    22981 cgcgtgcatc gccgccggct tcgtcgcgct ggtgctctac gtgcgccacg cacggcgcac
    23041 ggcgcatccg gtgctcgatc tcgacctgtt gaggctgcgg acgttctgga acggcgtggt
    23101 tgccggaagc gtgttccgcg ccggcctcgg tgcgacgccg ttcctgctgc cgttgctctt
    23161 ccagctcggc ttcggactcg acccgctgca gtcgggcctg ctcacgtgca tgactgcgat
    23221 cggcgcgatg ttcatcaaga cgctcaccgt cttcatcctg cgccgcttcg gctttcgcac
    23281 cgtgctgtcg gtcaacggcg ttgcggcatc gctgatcgtc atggccttcg gcctgttttc
    23341 gtcaaccacg ccgcacagcg tcgtcgcgat ggtgctgctg ttgtccgggt gcctgcgctc
    23401 gctgcagttc accgcgctga atgcgatctc gttcgccgac atcgcaccgg aaagaatgag
    23461 ccaggccgcg agcttctcga gcatggcgca gcgactgtcg caaagcgcag gcatcgcggc
    23521 gggcgcgtac ctgctgcagc tttcgagcac ggtgcaggga cacgcatcga tcgaggccgc
    23581 cgacttctgg cccgcgttcc tcggcatcgg gctcgtgtcg gcgctggcgc cgctcatgca
    23641 ccggcgattg gcgccgcaag ccggcatcga agtatcgggg cacggcgcgc cccctcgcca
    23701 gcgacgatga cggtgcgcgc gcgtcgtgcg gcgtcagcct cgcggctgca tcgcgcgcca
    23761 gacgcgttcc ggcgtgaggg gcatctgcag ctctcgcacg gcggatgcat gaccgtttcg
    23821 cgcgagcgcg tcggcaaccg cgttgacaac cgcgggcgtt gcgccgattg caccgagctc
    23881 gcccacgccc ttgacgccca tcgggttcgt gaggcaaggc gacgattcgt ccatcgtcat
    23941 cgcgaagcgg ccgatcatgc gcgcacgcgg catcgcgtaa tccatgaagc tcgcggtcag
    24001 cacctgaccc gattgcgcgt cgtacacgac gcgctcgcac agcgcctgcc cgatgccctg
    24061 cagcgcgccg ccttcgagct ggccgatcac gaccatcggg ttgatcacgc gcccgacatc
    24121 gttcaccgac cagtacgcgt cgagctcgac ctcgccggtg tcgggatcga cttccacttc
    24181 gcagacgtgg cacccattgg gccagctcgc cgcacccacg gagctggtcg acgcgacgac
    24241 gatgctttcg cccggctggg cttgcgcaag ctcgaaaagg ccgatgcgcc gatcggtgcc
    24301 ggcaatgccg aaaaccccat cgcggtattc gatatcggcc tcggccgcct cgagctgctg
    24361 cgccgcgagc gcgtgcgact tcgagagcgt gcgctctgac gcaacgcgga ccgccgagcc
    24421 gacgacaaac agcgaccgcg agccggcgct tccgaacccc gtcgcccggt cggtgtcccc
    24481 gaagcgcatg tcgatgagct cgggaggcac gccgaagacg tcgacgacca gctgcgtgaa
    24541 cgtcgtcgcg agactctggc ccatcggctg cgcggcggtg aagatctcga tgcggcctgc
    24601 acccgtcacc gtgacgtcga ccgtttcctc gaacacgtcg gcaccggtcc actcgaggaa
    24661 cgtcgcgatg ccctggccgc gcagcctgcc ccggcgtttc gcatcgccgg cgcgggcggc
    24721 gaagccgtgc cagtcgcaaa gcgcgagcgc ggcgtccatc accttcgcga actgcccgct
    24781 gtcgtacgtc ttgtccatcg cgttcgtgta cggcatctgc gcggggccga tcaggttgcg
    24841 ccggcgcagc tcgacgcggt cgatgcccgt ctgccgcgcc gcctcgtcga tcagccgctc
    24901 gatgatgtag atcgcttcgg gacgcccggc
//
LOCUS       DASSVX010000361         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_532451, whole genome shotgun sequence.
ACCESSION   DASSVX010000361 DASSVX010000000
VERSION     DASSVX010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532451"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1187)
                     /locus_tag="VFO53_15350"
     CDS             complement(45..1187)
                     /locus_tag="VFO53_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET8877705.1"
                     /translation="MSEDSEKIDIHRGLKGVYFDRSQVCFIDGRAGELLYRGYSIHDL
                     AERSTFEETCYLLMYGELPSADQLAQFDAALKAARTLPPAIHDVIRAVRDAHPMDVLR
                     TATSALAAFDPDVDDNSPDATLRKGVRLTSQVPMIIAAHEHMRHGREPVAPDPGLGHA
                     ANFLRMLKGAPASGDAARLMDIDMILHAEHGSNASAFTARVVAGTDANLHACVTAAIA
                     ALSGPAHGGAAENVMRMAQEIGDPANAAEYVRRKRANREAVMGFGHRVYRAEDPRARH
                     MRAGVEQLSRAMGEPKWYQILAALVDAMKPYARHGVNVNVDFYAGVVYFLNGIAEDLF
                     VPIFAAGRVPGWTVQVVEQLANNILIRPLTLYNGPAARPYVPIEKR"
     gene            complement(1263..1655)
                     /locus_tag="VFO53_15355"
     CDS             complement(1263..1655)
                     /locus_tag="VFO53_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HET8877706.1"
                     /translation="MDYATLKLIHQSAVAISFAGFFARGIGMLRDAAWVKHRLAKTLP
                     HLVDTVLIVSALWLAWILRLTPSNAPWIGAKIAGLFVYIGIGMVALRFGRTKGVRASA
                     WILAMLVFAWIVSVAITKDPRGFVSSVM"
     gene            complement(1781..4054)
                     /locus_tag="VFO53_15360"
     CDS             complement(1781..4054)
                     /locus_tag="VFO53_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HET8877707.1"
                     /translation="MKPNRKMWQWLGVVFVVSFAALGWLGREIYLAAPPIPNAVVTFD
                     GATLYTGDDVQRGQQAWLAAGGQQLGTVWGHGSYVAPDWSADWLHREAVALRELLARQ
                     RYNRAFDALAAADKAALDAEVKVQMRTNTYDASTKTLSIAPLRAQAIVQVAQHYDALF
                     GNSTALEKLREQYAMSDNALPDAADRHALTAFFFWSAWSSATDRPGEHGLSYTSNWPH
                     EPLVGNAMTTGSAMWSVVSVILLIAGIAGMIWYHSAHAEAADPEVPKSDPLVNAVATA
                     SMKATRKYFFTVIGLILVQVAMGAITAHYAVEGRAFFGFPLSDVLPYVVSRTVHTQFG
                     IFWIATAWLATGLYVAPLLSGHEPRFQKLGVNVLFWALILIVVGSTACGWLGTLQKRG
                     VDFSFWIGNQGLEFTSMGRFWQVLLFVGLLFWLLLLGRALWPALKRPSETRGLITMVF
                     LSATCIGGFYATSLVWGQHTHYAMIEYWRWWLVHLWVEGFFEVFATAVVGLIFTRLGL
                     VAASSANRAIVLETIVFLFGGILGTLHHLYWTGTPTSVIAVGAVFSALEVVPLALLGF
                     EGWQNYKRSRAAPWVAAYRWPILCFVAVGFWNTVGAGLLGFIINPPASLYYVQGLNLT
                     AAHGHAALFGVYGMLGIGLMLFCLRGLYARHLHADRYLAWTFWGLNIGLGMMVFMSLL
                     PAGIFQAYASVSEGLWFARSPAIVHSRMMETLVWLRVPGDIVFALGSVLLGVYALKLL
                     RSGPRGRRQVGGVPQPT"
BASE COUNT          801 a         1469 c         1405 g          674 t
ORIGIN      
        1 ggaccccgat ctggataatt acagctctga ccccgatctt cgagctatcg cttctcgatc
       61 ggcacgtacg gccgcgcagc gggcccgttg tacagcgtca gcggccggat caggatgttg
      121 ttcgcaagct gctcgaccac ctgcacggtc cagcccggga cgcgccccgc cgcgaagatc
      181 ggcacgaaaa ggtcctcggc gatgccgttc aggaaataca cgacgcctgc gtagaaatcg
      241 acgttcacgt tgaccccgtg gcgcgcgtac ggcttcatcg cgtcgacgag tgctgcgagg
      301 atctggtacc acttcggctc gcccatcgca cgcgacagct gctcgacgcc cgcgcgcatg
      361 tgccttgcgc gcgggtcttc tgcgcggtag acacggtggc caaagcccat caccgcttcc
      421 ctgttcgcgc gcttgcgcct gacgtactcg gcggcgttcg cgggatcgcc gatctcctgc
      481 gccatccgca tcacgttttc ggccgcgccg ccgtgcgcag ggccggatag tgctgcgatt
      541 gcggcggtga cgcacgcatg caggttggcg tcggtgccgg caacgacgcg ggcggtgaac
      601 gccgacgcgt tcgacccatg ctcggcgtgc aggatcatgt cgatgtccat gagccgcgcg
      661 gcgtcgccgc tcgccggtgc gcccttcagc atgcgcagga agttggcggc gtggccgaga
      721 ccgggatcgg gcgccaccgg ctcgcggccg tggcgcatgt gctcgtgcgc ggcaatgatc
      781 atcggcacct gcgacgtgag ccgcacgccc ttgcgcagcg tcgcatccgg cgaattgtcg
      841 tcgacgtcgg ggtcgaacgc ggcaagcgcc gacgtggcgg tgcgcagaac gtccatcgga
      901 tgggcgtcgc gcacggcgcg aatcacatcg tggatcgccg gcggcagcgt gcgcgcggcc
      961 ttcagcgcgg cgtcgaattg cgcgagttga tcggccgacg gcagctcacc gtacatcagc
     1021 agatagcacg tctcctcgaa cgtcgaccgc tccgcgagat cgtggatcga gtagccgcga
     1081 tagagaagct ctcccgcgcg gccgtcgatg aagcacacct gcgagcggtc gaagtacacg
     1141 cccttcaggc cgcggtggat gtcgatcttt tcgctgtctt cactcatcgt cgtagcctcg
     1201 cgcttgcacc ggattcgaag ctgcgacgat acaacatgcg cctcccgcaa ggccgtgcac
     1261 gatcacatca cggacgaaac gaagccgcgc gggtccttgg tgatcgcgac cgacacgatc
     1321 cacgcgaaga cgagcatcgc cagaatccac gccgacgcgc gcacgccctt cgtcctgccg
     1381 aaccgcaacg ccaccatccc gatgccgatg tagacgaaca gtccggcgat cttcgcgccg
     1441 atccacggcg cgttcgacgg cgtcaggcgc aggatccacg cgagccatag cgccgacacg
     1501 atgagcacgg tatcgaccag atgaggcagc gtcttcgcga gccggtgctt cacccacgcg
     1561 gcatcacgca gcatgccgat tccgcgcgcg aagaagccgg cgaacgagat cgcaaccgcc
     1621 gactggtgaa tgagcttgag cgtagcgtag tccatcgatg cgctttcctt tcccatgcac
     1681 tgcccggtgg tcgacgcgaa cggggcgagc cctgcggctc gccccgttcg attcgtgtct
     1741 cccgctccag cggcaaaccg cgccgccccg cccgctggcg ctacgtcggc tgcggcacgc
     1801 cgcccacctg gcgccgtccg cgcggaccgc tgcgcagcag cttcagtgca tagacgccga
     1861 gcagcacgct gccgagcgcg aacacgatgt cgcccggcac ccgcagccac acgagcgttt
     1921 ccatcatgcg cgaatgcacg atcgccggcg agcgcgcgaa ccacaggcct tcggacacgc
     1981 tggcataggc ctggaaaatc cccgccggca gcagcgacat gaacaccatc ataccgagcc
     2041 cgatgttgag accccagaac gtccatgcca gatagcgatc ggcatgcaga tgccgtgcat
     2101 agagaccgcg caggcagaac agcatcaatc cgatgccgag cataccgtac acgccgaaca
     2161 gcgccgcatg cccgtgtgcg gcggtcaggt tcaggccctg cacgtaatag agcgacgccg
     2221 gcgggttgat gatgaagccg agcaatcccg cgcccacggt gttccagaag ccgaccgcaa
     2281 cgaagcacag gatcggccag cgatacgcgg cgacccacgg cgcggcgcgg ctgcgcttgt
     2341 agttctgcca gccctcgaag ccgagcagcg cgagcgggac cacttcgagc gcggaaaaca
     2401 cggcgcccac ggcgatcacc gacgtgggcg ttcccgtcca gtacaggtga tgcagggtgc
     2461 cgagaatgcc gccgaacagg aacacgatcg tctcgagcac gatggcgcga ttggcgctcg
     2521 acgccgcgac gagcccgagc cgcgtgaaga tgaggccgac gacggcggtc gcgaacactt
     2581 cgaagaagcc ctcgacccac aggtgtacga gccaccagcg ccagtattcg atcatcgcgt
     2641 agtgcgtgtg ttgtccccac acgagcgatg tcgcatagaa gccgccgatg caggtcgcgg
     2701 acaggaacac catcgtgatc aatccgcgcg tttccgacgg acgcttcagc gcaggccaca
     2761 acgcgcggcc gagcagcagc agccagaaca gcaggccgac gaacagcagc acctgccaga
     2821 agcggcccat gctcgtgaac tcgagaccct ggttgccgat ccagaagctg aagtcgacgc
     2881 cgcgcttctg cagcgtgccg agccagccgc aggcggtcga gccgacgacg atcaggatca
     2941 gcgcccagaa gagcacgttc acgccgagct tctggaaccg gggctcgtgg ccggaaagaa
     3001 gcggcgccac gtaaagcccg gtcgcgagcc acgcggtggc gatccagaag atgccgaact
     3061 gcgtgtgcac ggtgcggctc acgacgtacg gcagcacgtc ggacagcgga aagccgaaga
     3121 acgcgcgccc ctcgaccgcg taatgcgcgg tgatcgcgcc catcgccacc tgcacgagga
     3181 tcaggccgat cacggtaaag aagtacttgc gtgtcgcctt catcgatgcc gttgcaaccg
     3241 cgttgaccag cggatcggat ttcggcacct cgggatcggc ggcctccgcg tgtgccgagt
     3301 ggtaccagat catgcctgcg atgccggcga tgagcaggat gaccgacacg accgaccaca
     3361 tcgcgctgcc ggtggtcatc gcattaccca cgagcggctc gtgcggccag ttgctggtgt
     3421 acgaaaggcc gtgttctccg ggacgatcgg ttgccgacga ccatgccgac cagaagaaga
     3481 atgcggtcag cgcatgccgg tcggcggcgt cggggagcgc gttgtcgctc atcgcatact
     3541 gctcgcgcaa cttttcgagc gccgtcgaat tgccgaacag cgcgtcgtaa tgctgcgcga
     3601 cctgcacgat cgcctgcgcg cgaagcggcg caatggacag tgtcttcgtc gatgcgtcgt
     3661 acgtgttcgt gcgcatctgt accttgacct cggcgtcgag tgccgccttg tcggcggccg
     3721 ccagcgcatc gaacgcgcgg ttgtagcgct gccgcgcgag cagctcgcga agcgcaacgg
     3781 cttcgcgatg cagccagtcg gcggaccagt cgggggcgac gtagctgcca tgcccccaca
     3841 cggtgccgag ctgctggcca ccggctgcaa gccatgcctg ctggccgcgc tgcacgtcgt
     3901 cgccggtata gagcgtcgcg ccgtcgaacg tgacgacggc attcgggatc ggcggcgccg
     3961 cgagatagat ttcgcggccg agccagccga gtgctgcgaa cgacactacg aagacaacgc
     4021 cgagccattg ccacattttg cggttcggtt tcacttgggc actccctctt ccggttgcga
     4081 atcgatggag gaattctagg ggcgccgata cgcaaaaccg cgcctcgcgc aagccatttc
     4141 gtgctttgtt gatgccggtc aaaccgcgcg gtgcgagcca cttgtggaag aatgcgggcg
     4201 tcgaatgact ggaggatcac cggatgacag ccgcgtttcg catgaccgcg ttcctttcgc
     4261 tgctcgccgc tgcgtcgctt gcgcttgcgc agccggcgaa cagcgctcca ggcgagccgc
     4321 ccgttgccgc agcgcaggca cgtgcccag
//
LOCUS       DASSVX010000362         7531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_932707, whole genome shotgun sequence.
ACCESSION   DASSVX010000362 DASSVX010000000
VERSION     DASSVX010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932707"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..858
                     /gene="coq7"
                     /locus_tag="VFO53_15365"
     CDS             238..858
                     /gene="coq7"
                     /locus_tag="VFO53_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HET8877708.1"
                     /translation="MIDTLIVGFDRALRTLSGTVAAARATPAAGTPEPKLEPHEQRDA
                     AGLMRVNHTGEVCAQALYSAQAIVAREPEVRERYADAARDEEDHLAWTQARLTELGAR
                     PSLLNPLWYAGSFAIGIIGGIAGDRTNLGFVVETERQVEEHLTAHIERLPPNDTKSRA
                     IVTQMRDDESRHGAMALEAGALPLPFPIRHAMRAAADLMRAVAYRI"
     gene            complement(879..2183)
                     /gene="cca"
                     /locus_tag="VFO53_15370"
     CDS             complement(879..2183)
                     /gene="cca"
                     /locus_tag="VFO53_15370"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259778.1"
                     /note="catalyzes the addition and repair of the essential
                     3'-terminal CCA sequence in tRNAs without using a nucleic
                     acid template; phosphohydrolase activities include
                     hydrolysis of pyrophosphate, 5'-nucleoside tri- and
                     diphosphates, NADP, and 2'-AMP with the production of Pi,
                     metal-dependent phosphodiesterase activity for 2',3'-cAMP,
                     2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic
                     substrates with the formation of 2'-nucleotides and
                     3'-nucleotides; these phosphohydrolase activities are
                     probably involved in the repair of the tRNA 3'-CCA
                     terminus degraded by intracellular RNases; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HET8877709.1"
                     /translation="MTRPLPQPVRIYRVGGSVRDELLQIPSGDDDWVVVGATPEIMVA
                     SGFTPVGKDFPVFLHPDTHDEYALARTERKHGRGYRGFEFFATPDVTLEDDLRRRDLT
                     INAMARAPDGTLIDPHGGADDLRAGILRHVSPAFAEDPLRVLRVARFAARFGFAIADG
                     TLALMRDIVAAGELATLSPERVWQELSRGLSEEHPWRMLRALRAVGALAALLPEIDAL
                     YATKHDGDDIGALTERALDWAAQHRFVLPVRYAALMTRLGETNAANAARNPSSRHGSD
                     SVLTAAKRSARVAVARVDAVSRRLKVPLECRDSARIAARFVVDATRANTLAPARLLDI
                     IVAADALRRSDRLDWLIDSACAYAAAEQHADCAAPLLRDALAIIRGVDASTIARDAIA
                     RAASQQAPGATATVGDAIGTALRNARLAALRRWRMARRAESR"
     gene            complement(2180..3127)
                     /locus_tag="VFO53_15375"
     CDS             complement(2180..3127)
                     /locus_tag="VFO53_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HET8877710.1"
                     /translation="MIEGRQRVLVLGGSGFVGRYVVAQLARAGHFAIVLTRRRARARH
                     LFLLPTVQVIEGDPYDAATLARAARGTTAAINLAGVLHEHVRATFERVHVELPRALVQ
                     ACRAAGIARLLHMSALGASREAPSRYLRSKAEGEEVIAASGLATTIFRPSVIFGREDT
                     FLSLFAKLSRWFPVLPLAGANARFQPVYVADVAGCFASALIDNETIGERYGLCGPKTY
                     TLAELVRYVGEVTGHPRPIVPLSPALGTLQARMMEWLPGPLLTRDNLASMQVDNVCDT
                     AFPARFGIAPTALEAVAPEYLAPSAQHSRFDLFRAHGGR"
     gene            complement(3138..5102)
                     /locus_tag="VFO53_15380"
     CDS             complement(3138..5102)
                     /locus_tag="VFO53_15380"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET8877711.1"
                     /translation="MRFALHRLHPLAIAVVACASLFAGGTACAQSDADFVAAKAAFDR
                     GDLRELDALAPALSGHVLARYVEYWQLKSRLDGATPEAIQSFVTRYPDGPLADRLRVD
                     WLKVLGKRGDWNRFALDYPPASGEDTELACYGAQYKYHRDGPVALVAAKSLWFTGSST
                     PDACEPVFAALLARGDLSFADRRTRFRLAATAGNARLAQAMAADLPPNDRIAARDLLA
                     VDRDPLRSVAKGQFAWKTAAGRELALYALERAARKDAGEARAAWTKWRLHFDAADRNY
                     GSLRIAYHAARQLHPLANQWFDELPHLALTPDEQAWRVRAALRAGAWRDVKSAIEALP
                     VNDQQEPAWRYWRARALAMGTGGDEARAIYASLAGSPTYYGLLAAEALGNTSTLPGVV
                     STPLSTDDVALTAFGKRPDVIRAVKLARLDARLDAIREWNQAVRGMNDDALLLAAEYA
                     RRAGLYDRAIYTAERTASRHDFSLRYMTPFRTEFGNAARELGVDEELLYGIARQESRF
                     AADIVSSAGAVGLMQLMPGTARWVAKQMTVVDYSPSRIADVDLNTKFGAFYFKYWQDR
                     LGQLPALAAAAYNAGPGRAQAWRPATGALEGAIWVETIPFNETRDYVKRVLANTVVYA
                     QALDRPYVGLTKRLGVVTPRDSAVIAAGDP"
     gene            5255..5761
                     /gene="purE"
                     /locus_tag="VFO53_15385"
     CDS             5255..5761
                     /gene="purE"
                     /locus_tag="VFO53_15385"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET8877712.1"
                     /translation="MAQRKAAPQVGLVMGSDSDWDVMKHAATQLDAFGVTYEARVVSA
                     HRMPDEMFEYAEDAGPRGLRAIIAGAGGAAHLPGMLASKTTVPVLGVPVPSKYMRGED
                     SLLSIVQMPAGIPVATFAIGEAGAANAALFAISLLAATDAALATKLVAFRQKQTTTAR
                     SMRVPPES"
     gene            5758..6945
                     /locus_tag="VFO53_15390"
     CDS             5758..6945
                     /locus_tag="VFO53_15390"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET8877713.1"
                     /translation="MSETPRTASGHAFPPGAWLGLLGGGQLGRMFCMAAQSLGFRVLV
                     LDPGSDSPAGSVADRHLAADYLDPPSLAALRALCVAATTEFENVPAAALEFLARELRV
                     TPGAASVAIAQDRIREKTFLSDHGFAVAPFAVLNSATDVAAVDDALLPGIVKSARYGY
                     DGKGQVRVATNVEVGAAFEAMQGPAVLERLIDLAAELSVIVARDDAGNVVSWPVAENR
                     HRGGILDVSILPARIDSALADEARAIATAVATALEYRGVLCVELFVTREERLLVNEIA
                     PRPHNSGHYTIDACVTSQFEQQARILAALPLGDTAQHTPAVMLNLLGDIWFDAGAAEP
                     REPDWTRVLAHPRAKLHLYGKREPRRGRKMGHVTCLGATIDDALATAAAIKLALDIPG
                     DVG"
     gene            6942..>7531
                     /locus_tag="VFO53_15395"
     CDS             6942..>7531
                     /locus_tag="VFO53_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET8877714.1"
                     /translation="MNGAPSPPSDVDPPLAGVRVLDLTRLLPGPMCTLYLADLGADVV
                     KVEDTGAGDYARTLAIRPASKRDRTTAWFNAINRNKRSLAIDLKHADGNAAFLALAKE
                     ADVIVEGFRPGVVAALRIDYDTIRAINPRVVYCSLSGYGQTGPRAAVAGHDINYLGYA
                     GTLNYTGQRGGAPALANLQIADLLGGAATAAIGILAA"
BASE COUNT         1293 a         2554 c         2518 g         1166 t
ORIGIN      
        1 gtcaagatcg acaacggcgg tcaggcgtcg tacacgttca acatcaacgc gtatccgatc
       61 gccatcggtg gcaagcccca aggcttcgtg cttggcatga tccacctgtt ctaagcaata
      121 tcgcgttccc ggtcgccggg acacgttcac agcgggcgcc ttcgggcgcc cgttgtcatt
      181 ttcgtgcctg cgagctcgac gttctcacga ctcggccatg cggcgtatca tcgaccgatg
      241 atcgacactt tgatcgttgg cttcgatcgc gcattacgca cgctgtcagg caccgttgca
      301 gcggcgcgtg cgacaccggc tgccggaacg ccggagccta agctcgagcc tcacgagcag
      361 cgcgacgctg cgggcttgat gcgcgtcaat cacacgggtg aagtgtgcgc gcaggcactg
      421 tactcggcac aggcgatcgt tgcgcgcgag cccgaagtgc gtgaacgcta tgcggatgcc
      481 gcacgcgacg aggaggatca cctcgcgtgg acgcaggcgc gtctgactga gcttggtgcc
      541 cgcccgtcgc tcctgaatcc gctgtggtac gcgggatcgt ttgcgatcgg catcatcggc
      601 ggcatcgccg gcgatcgaac caatctcggc ttcgtcgtcg aaacggaacg gcaggttgaa
      661 gagcatctga ccgcgcatat cgaacgactg ccgccgaacg acacgaaaag ccgcgcgatc
      721 gtcacgcaaa tgcgcgatga cgaatcgcgg catggcgcga tggcactcga agcgggagcg
      781 ttgccgctgc cgttcccgat tcgccatgcg atgcgcgccg ctgcggatct gatgcgcgca
      841 gtcgcgtacc ggatctgatc gccggcccgc ccccggcttt accgactttc cgctcgacgc
      901 gccatacgcc agcggcgaag tgctgcgagc cgcgcgttgc gcaacgcggt gccgatcgca
      961 tcaccgacgg tggccgtagc acccggcgct tgctgcgacg ctgcgcgcgc gatcgcatcc
     1021 cgtgcgatcg tcgacgcatc gacaccgcgg atgatcgcga gcgcatcgcg caggagcggc
     1081 gctgcacagt cggcatgctg ctccgccgcg gcgtacgcgc acgccgagtc gatcagccag
     1141 tcgagccgat ccgagcgccg cagcgcatcg gccgcgacga tgatgtcgag cagtcgagcg
     1201 ggcgcgagcg tgttggcgcg cgtcgcatcg acgacgaatc gcgcggcgat acgcgcactg
     1261 tcgcgacatt cgagcggaac cttcaaacgc cgcgacacag cgtccacgcg tgcaacggcg
     1321 acacgcgccg agcgcttggc tgcggtgagt acggaatcgc tcccatggcg gctcgaggga
     1381 ttgcgcgcag cgtttgctgc attcgtctcg ccgagccgcg tcatcaacgc cgcataacgg
     1441 acgggcagca cgaaacgatg ctgcgcggcc cagtcgagcg cgcgctcggt cagcgctcca
     1501 atatcgtcgc cgtcgtgctt cgtcgcatag agcgcgtcga tctccggcag cagcgcagcg
     1561 agcgcgccca ctgcgcgaag cgcgcgcaac atccgccatg gatgttcctc cgacaatcca
     1621 cgcgacaact cctgccaaac gcgctcgggc gagagcgttg caagctcgcc cgccgcaacg
     1681 atgtcgcgca tgagtgcgag cgtcccatcg gcgatcgcga atccgaatcg cgcggcaaat
     1741 cgtgcaacgc gcagcacgcg aagcggatcc tcggcgaacg ccggcgacac gtgacgcagg
     1801 atgccggcgc gcagatcgtc ggccccgcca tgcggatcga tcagcgtccc gtcgggcgcg
     1861 cgcgccatcg cgttgatcgt caaatcgcgt cgccgcagat cgtcctcgag cgtgacgtcc
     1921 ggggtcgcga agaattcgaa tccgcggtag ccacggccat gcttgcgctc ggtgcgcgcg
     1981 agcgcgtatt cgtcgtgcgt atcggggtgc aggaagaccg gaaagtcctt gccgaccggc
     2041 gtgaagcccg acgcgaccat gatctccggt gtcgcgccga cgacgaccca atcgtcatcg
     2101 cccgagggga tctgcagcaa ctcgtcgcgc accgagccac cgacgcgata gatgcgcacc
     2161 ggttgcggca acggccgcgt catcgtccgc cgtgggcgcg gaacaggtcg aagcggctgt
     2221 gttgcgcgga gggagcgagg tattcgggag cgacggcctc gagcgccgtg ggcgcgatac
     2281 cgaagcgcgc cgggaatgcg gtatcgcaaa cgttgtcgac ctgcatcgac gccagattgt
     2341 cgcgcgtgag gagcgggccc ggcagccatt ccatcatccg cgcctgcagc gttccaagcg
     2401 ccggtgaaag cggcacgatg ggccgcggat gaccggtgac ttcgccgaca tagcgcacga
     2461 gctccgcaag cgtgtacgtc ttcggtccgc acaggccgta gcgctcgccg atcgtttcgt
     2521 tgtcgatcag cgcggacgca aagcagccgg cgacgtcggc cacgtagacg ggttggaacc
     2581 gcgcgttcgc acccgcgagc ggcagcaccg gaaaccagcg agagagcttc gcgaacaggc
     2641 tgaggaacgt gtcctcgcgt ccgaagatca ccgacggccg aaagatcgtt gtcgcgaggc
     2701 ccgatgcggc gatgacttcc tcgccttcgg ccttggagcg caggtatcgg ctcggagcct
     2761 cgcgcgaggc accgagcgcg ctcatgtgca gcagccgggc gatgccggct gcgcgacacg
     2821 cctgcacgag ggcgcgcggc agctcgacgt gaacccgctc gaacgtcgca cggacatgct
     2881 cgtgcagcac gcccgcgaga ttgatcgccg ccgtcgtgcc ccgagcagcg cgcgcaagcg
     2941 tcgccgcgtc gtagggatcg ccttcgatga cctgcacggt cggcaacagg aacaggtgcc
     3001 gggcgcgcgc gcgccgccgc gtcagcacga tcgcaaaatg gccggcccgc gccagctgcg
     3061 cgacgacgta gcggccgacg aacccgctgc cgcccagcac cagcacgcgt tggcggccct
     3121 cgatcatccg gcagcgctca aggatcgccg gcggcgatga cggcggagtc gcgcggcgtg
     3181 acgacaccga gccgcttggt gagaccgacg tacgggcggt cgagcgcttg cgcgtagacg
     3241 acggtattgg cgagcacccg cttcacgtaa tcgcgcgttt cgttgaatgg aatcgtctcg
     3301 acccagatcg caccttcgag cgcgccggtc gccgggcgcc acgcctgtgc acggcccgga
     3361 cccgcattgt aagcggcagc ggccagcgcc ggcagttggc cgagcctgtc ctgccagtac
     3421 ttgaaataga acgcgccgaa cttcgtgttg agatcgacgt ctgcgatccg tgacggcgaa
     3481 tagtcgacga cagtcatctg cttcgcgacc cagcgcgcgg tgccgggcat caattgcatc
     3541 aacccgacgg cgcctgccga cgacacgata tcggcggcaa aacgcgattc ctgccgcgcg
     3601 atgccataca gaagctcctc gtccacgccg agctcgcgtg cggcgttacc gaactcggtg
     3661 cgaaacggcg tcatgtaacg cagcgagaaa tcgtgtcgcg acgccgtgcg ctcggccgtg
     3721 tagatcgcgc gatcgtagag gcccgcgcga cgcgcgtatt cggccgcgag caggagcgcg
     3781 tcgtcgttca tgccacgcac cgcctggttc cattcgcgga tcgcgtcgag ccgtgcatcg
     3841 agccgcgcga gcttgaccgc gcgaatcacg tccgggcgct tgccgaatgc agtcagcgcg
     3901 acatcgtcgg ttgaaagcgg cgtgctcacg acacctggca acgtcgacgt gttaccgaga
     3961 gcctccgccg ccagcaggcc gtagtaagtg ggactgcccg cgagcgaggc gtaaatggcg
     4021 cgcgcctcat cgccgccggt tcccatcgcg agcgcgcgag cgcgccagta gcgccacgcc
     4081 ggttcctgct gatcgttgac gggcagcgct tcgatcgcgg acttcacgtc gcgccacgcg
     4141 ccggcgcgta acgccgcgcg cacgcgccac gcctgctcat cgggtgtgag tgccaaatgc
     4201 ggcagctcgt cgaaccactg gtttgcaagc ggatgaagct gccgtgcagc gtgatacgca
     4261 atgcgcaggc tgccgtaatt gcgatcggca gcgtcgaaat gcaggcgcca tttggtccac
     4321 gcggcccgcg cttcaccggc atccttgcgc gccgcgcgct cgagcgcata aagcgcgagc
     4381 tcgcgacctg cggcggtctt ccacgcgaat tgacctttgg caacgcttcg cagcggatcg
     4441 cgatcgacgg cgaggagatc acgcgccgca atgcggtcgt tcggcggcag gtcggcggcc
     4501 atcgcctgcg ctagccgcgc attgcctgcg gtagccgcga ggcggaagcg cgtgcggcgg
     4561 tcggcgaacg acagatcacc gcgtgcgagc agcgccgcga acaccggctc gcacgcatcc
     4621 ggcgtcgacg atccggtgaa ccacaacgac ttggctgcca cgagtgcgac ggggccatcg
     4681 cggtgatact tgtattgggc gccgtagcac gcaagctcgg tgtcttcgcc gctcgcaggc
     4741 gggtagtcga gcgcgaagcg gttccagtcg ccgcgcttgc cgagcacctt caaccaatcg
     4801 acgcgcagcc ggtccgcgag cggcccatcg ggatagcgcg tgacgaacga ctggattgcc
     4861 tccggcgtcg caccgtcgag ccgcgacttc aactgccagt actcgacgta gcgcgcgagc
     4921 acgtgccccg acaacgccgg cgccagcgca tcgagctccc gcaagtcgcc gcggtcgaac
     4981 gcggctttcg ccgcaacgaa gtccgcatcc gattgcgcgc acgcagttcc accggcgaac
     5041 aacgacgcgc atgcgacgac ggcgatggcg agcggatgaa gacggtgcag tgcaaatctc
     5101 atgcgatcaa ttgtcgctta tgcacgccgc gcgcttcaac gattacgcgc tcatacgaga
     5161 tgaacggcga tgaaagccgc cagacggcgc aacagttgtt aaaatcgcgc acgttctcgc
     5221 tacgggcaag tgcccgcaca gaggccctca cgagatggcg cagcgcaagg cggcaccgca
     5281 ggtcggcctc gtcatgggca gcgacagcga ctgggatgtg atgaaacacg ccgcgacgca
     5341 gctcgatgcg ttcggtgtca cctacgaggc gcgcgtcgtc tcggcgcatc ggatgcccga
     5401 cgagatgttc gagtacgccg aggatgcggg tccgcgcgga ttgcgcgcga tcatcgcggg
     5461 cgccgggggc gcggcacacc tgcccggcat gctcgcgtcg aaaacgacgg tgcctgtcct
     5521 gggcgttccg gtcccatcga aatacatgcg cggcgaggat tcgctattgt cgatcgtcca
     5581 gatgcccgcc ggcatcccgg ttgcgacgtt cgcgattggc gaagcgggcg ccgcaaacgc
     5641 cgcgttgttc gcgatctcac tgctggcggc aacggatgca gcactcgcga caaagctcgt
     5701 cgcattccgg cagaagcaga cgacaacggc acgctcgatg cgcgtcccgc cggagtcgtg
     5761 agcgaaacgc cgcgcacggc ttcaggccac gcgtttccac cgggcgcctg gctcggactg
     5821 ctcggcggcg gacagctcgg gcggatgttc tgcatggcgg cacaaagcct cggctttcgc
     5881 gtgctcgtgc tcgatcctgg cagcgacagc cccgccggca gcgttgcgga ccgacatctc
     5941 gccgccgact atctcgatcc gccgtcgctt gcagccctcc gcgcgctgtg cgttgccgcg
     6001 acgaccgaat tcgagaacgt gccggcggcg gcactcgagt tcctcgctcg cgagcttcgc
     6061 gtcacaccgg gggctgcgag tgtcgcgatc gcgcaggacc ggattcgcga gaagacgttc
     6121 ctcagcgacc acggctttgc cgtcgcgccg ttcgccgtgc tcaacagtgc aacagatgtt
     6181 gcggcggtcg acgatgcgct gctgcccggc atcgtcaaga gcgcccggta cggctacgac
     6241 ggcaagggtc aggtacgcgt cgcgacaaac gtcgaagtcg gtgctgcctt cgaagcgatg
     6301 caggggccgg ccgtgctcga gcgtctgatc gacctcgccg cggagctgtc ggtgatcgtc
     6361 gctcgagatg acgccggcaa cgtcgtgtcg tggccggttg cggagaaccg ccatcgcggt
     6421 ggaatcctcg acgtatcgat cctgccggcg cgaatcgaca gcgcgctcgc cgacgaagca
     6481 cgcgcgatcg ccactgcggt cgctacagcg ctcgaatatc gcggtgtgct gtgcgtcgag
     6541 cttttcgtca cccgcgaaga gcggctgctc gtcaacgaga ttgcgccgcg gccgcacaac
     6601 agcggccatt acacgatcga cgcgtgcgtg acgtcgcagt tcgagcagca ggcgcgcatc
     6661 ctcgcggcgc tgccgctcgg cgacaccgcg cagcacacac cggccgtcat gctgaacctg
     6721 ctcggcgaca tctggttcga cgccggcgcc gccgagccgc gtgaacctga ttggacgcgc
     6781 gtgctcgccc atccgcgggc gaagctgcat ctctatggca agcgtgagcc gcggcgcggc
     6841 cgcaagatgg gtcacgtgac gtgtcttggc gccaccatcg acgatgcact cgcgacggcg
     6901 gcggcgatca agctggcact cgacattccc ggcgacgtcg ggtgaacggc gcgccatcgc
     6961 ctccaagcga cgtcgatccg ccgctcgccg gcgtgcgcgt gctcgacttg acgcgcctct
     7021 taccagggcc gatgtgcacg ctgtaccttg ccgatctcgg ggccgatgtc gtcaaggtcg
     7081 aggataccgg cgccggcgac tatgcgcgaa cgctcgctat ccgccccgcg agcaagcgcg
     7141 accgaacgac cgcgtggttc aacgcgatca atcgcaacaa gcgctcgctg gcgatcgatc
     7201 tgaagcacgc cgacggtaac gcggcgttcc tggccctggc gaaggaggct gacgtcatcg
     7261 tcgaaggctt ccggcccggg gtcgtcgccg cattgcggat cgactacgac acgattcgcg
     7321 cgatcaaccc gcgtgtcgtc tactgttcgt tgtcgggcta tggccagacg ggtccgcgcg
     7381 cggcggttgc cgggcacgac atcaattacc tcggctacgc aggcacgctg aactacaccg
     7441 ggcagcgcgg cggcgcgccg gcgcttgcga acttgcagat cgcggatctg ctgggcggcg
     7501 ccgccacggc cgcgatcggc atcctcgccg c
//
LOCUS       DASSVX010000363        13460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_299395, whole genome shotgun sequence.
ACCESSION   DASSVX010000363 DASSVX010000000
VERSION     DASSVX010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 13460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13460
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299395"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFO53_15400"
     CDS             complement(<1..271)
                     /locus_tag="VFO53_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877715.1"
                     /translation="MYNWIACSGVIIFAIFAIAAKSGIAGAGDDLSAGDRSPGESFAF
                     IFGGERLRAACLRAPLSALAVAAALLAMFRSFARDRDAHPEHESRR"
     gene            237..740
                     /locus_tag="VFO53_15405"
     CDS             237..740
                     /locus_tag="VFO53_15405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HET8877716.1"
                     /translation="MMTPEQAIQLYKANAALALEVINTAIEGTARLRRKQLEGAAEAS
                     EFQHRHAQSAAHARDPQALVAAGQGAAQEAMERSLRYWSEMFDLIVEIQKRLFTLMEE
                     QMEGVPGVKETRAAMTMLPDMGQMQKVVSAMQGVVSSGESTFASMQRVMGDFAKLAQG
                     GVPGGRR"
     gene            737..907
                     /locus_tag="VFO53_15410"
     CDS             737..907
                     /locus_tag="VFO53_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877717.1"
                     /translation="MSAAKRAEPGVTASGRLAPAIGRSLRHLVPVAIALLLAGCVPIG
                     VRVQNMLALWQG"
     gene            988..1314
                     /locus_tag="VFO53_15415"
     CDS             988..1314
                     /locus_tag="VFO53_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877718.1"
                     /translation="MDRRLDWIVRKGALAAALYLAIVGGIDWLQYGIAACVWWPLATN
                     VWAVPVAAPWRSAGPAPLPPTAAMTFDLAVLGSLFLAHWYWTAFAYAISCGIAALVHA
                     RAVSKP"
     gene            1493..4414
                     /locus_tag="VFO53_15420"
     CDS             1493..4414
                     /locus_tag="VFO53_15420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET8877719.1"
                     /translation="MAPRVSKGISLFDALRHAPAHRPSLAWLALVLGFAASFGVLLWV
                     NGVVERKAQAAFAEDASDTASEVARRARAYADVLFAVRGLFDVSQNVTRAQFHRFARG
                     LDFERRYSGLANLSFAYRVRSDERNAFERRMRKEGGELSQDLPPFAIKASEARPEHIV
                     LSYVDPIEEGSQVLGFDLSDDPARLAAIESARDSGGLSTTPSIAKLTDPKVHPSSFVL
                     RLAVYDGGVIPPTIEARRRAFVGVAGATIRLPDVIKASEQATALGNIRVRVEDVTGRP
                     SVTKGTLLFDSGAPSGQTSPILYAKYATANRFIMGDRILSVAITPLAKPQPRGLDVFL
                     PYIAGALTMIGALLVFGLIRALAKAEVRGESLRRSLRGLEFHRARLAETQEIANIGGF
                     EWDTRASAQVWSDQLYRILGREVGDPPHPDHDFFFANAVHHADAPAVREALRRVLHDG
                     GPVALDCRIVRPDGAERIVSTLTRLEAADDGARTRVVGTVRDITGEWRAAERERAQLH
                     FIQTVIDAIPIPVFQKDTEGRFRICNDAWCRFVGLSREMLIGRTAEEVLPGTDFDAVR
                     VRDTELLARVGTDSIEVDLPNAAGKLRRVMLHKASYPARDGAIAGLVGAAFDITELRQ
                     TEDELERTIAQLDRRNCTAMLLGEFGEVLQACLDLEDAYEAVEKYLPRLLPRSAGIVY
                     RVGAGRGNAERCATWGAPVGLKDVIATSDCVAVRRGQPRCVEQSEDQLNCRHFTAPPA
                     SYACVPLSSHGELLGLLHVQASAGCAGACDTEAEWPALRSACEQISLALANLAIRETL
                     REQATRDKLTGLYNRHYLNARFEQDLARAHRGESPIGIVLLDIDHFKRFNDTFGHAAG
                     DHVLREVAAVLMSVTRRSDVACRYGGEEFLLLMADASREIAIRRAEELRLAVKGLRLE
                     WQGHPLGELTISAGVAALPADGTEAEILVRRADQALYRAKELGRDRVEAALPFAAFAN
                     G"
     gene            complement(5055..5141)
                     /locus_tag="VFO53_15425"
     tRNA            complement(5055..5141)
                     /locus_tag="VFO53_15425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5105..5107),aa:Leu,seq:taa)
     gene            5351..5785
                     /locus_tag="VFO53_15430"
     CDS             5351..5785
                     /locus_tag="VFO53_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HET8877720.1"
                     /translation="MNAFLKSKSLMQVSCWSLAAALACTSAAALAAAMNVKLSGAEEV
                     PAVQSSGSGSGSITIGDDGSVSGSVTASGFTPTAAHIHEGKAGANGKVIVPFTKEGDK
                     FSAPAGAKLTPDQMKAFKEGDLYVNIHSAAHPGGEVRAQLKP"
     gene            complement(5873..6304)
                     /locus_tag="VFO53_15435"
     CDS             complement(5873..6304)
                     /locus_tag="VFO53_15435"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET8877721.1"
                     /translation="MLKVLLPVDGSASALNATRTLIETLAWYREAPAVELCTVHLPLP
                     RVPNMGQFVSKEMVQKYYADESDAMLRPSRALLDAGGVPYTVQRLVGPVAETIVERAK
                     QAGSDMIFMGTRGMSALANMAMGSVTTRVLHLAHIPVVLIH"
     gene            6471..7220
                     /locus_tag="VFO53_15440"
     CDS             6471..7220
                     /locus_tag="VFO53_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015114301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HET8877722.1"
                     /translation="MHDQTPDLDALFDDAPKPDRTFDPSDVGHLPPPAQRYLRHAIAP
                     DTPLASAVRLRMHGAIRLKRWHPFKAEQVIVRDRGMIWRARVKLAGATIRGFDRFVDG
                     AGAMQWTLCGLLPVVRASGPDVTRSSAGRFAAESVWLPSLFCTDQVSWRADDHDGVAH
                     AHLSVDAHDEDLELTLNGGSLQSVALPRWGNPDGGNFREVEFGAAVDQEATFGGYTIP
                     VRLRVGWYFGTGRFEGEGKFFHATIDDAVFR"
     gene            7263..8537
                     /locus_tag="VFO53_15445"
     CDS             7263..8537
                     /locus_tag="VFO53_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HET8877723.1"
                     /translation="MRSPARGSIAAAHALLALALIALAAAGFLASFRVAWLIAASLCA
                     AAAALVALFLLSAETRGRRMADLELRRSEGRLRGILDSAMDSIITVDENQRVVLFNDA
                     AEAMFGWRRDEAMGTALDNFIPERFRHVHAAHVERFGETRTAARRMGGSLRIVTGLRR
                     NGDEFPIDASISQIEESGKRFYTVILRDITARLASEEALRRSRDDLQALGAAAHMTRE
                     QEKSRIARELHDELGQLLTMLQMDVAWCKEALPYGHEALETRLERMEKLLKSTIAATR
                     RIAADLRPLMLDDLGLVPSLEWLVENFSQRTGIACALSVGERDLALPKAHANAIFRIV
                     QESLTNIAKHADASRAEIALERDEDELVVRVADDGVGFSPQAPRKPGSLGLFGLRERA
                     SLMGGEASIVSAPGEGTTIVVRLPVEAPMASA"
     gene            8534..9184
                     /locus_tag="VFO53_15450"
     CDS             8534..9184
                     /locus_tag="VFO53_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8877724.1"
                     /translation="MIRVVIADDHQILREGLKQLLLAAGDLDVVGDAGDGHEVLERVR
                     TLGFDVLLLDMSMPGKSGVELIRQVKAEKPKLRILVLSMHEEHQYAVRAIRAGASGYL
                     TKESAAAQLVAAIRKIAAGGAFISAEVAERLALDAMPHAEGPLHATLSDREYQVFRLL
                     VTGRSVSDIAEQLHLSAKTISTHKARLMEKLQVDNNAALVRYAVDHRLQDDPNAPA"
     gene            9315..9701
                     /locus_tag="VFO53_15455"
     CDS             9315..9701
                     /locus_tag="VFO53_15455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8877725.1"
                     /translation="MNTPARTHVFIVDDLPSMRERLRELVDGVDGIEVVGEAGTPGEA
                     IDGIRRTHPDYVLLDYQLIGGTGVDVLRAVHPDAPDIAFIVLTNHATAQHRRACLDAG
                     ARFFFDKSTEFGRVCRAIAESRPPRP"
     gene            9710..10498
                     /locus_tag="VFO53_15460"
     CDS             9710..10498
                     /locus_tag="VFO53_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8877726.1"
                     /translation="MLVSTAVRVREPSGPQPAVTARPLLRDVSRDVASCTVCTLRRIC
                     MPVDIQDAEARKLFERLVTTRIRLRKGDVLFRAGDRFTALFAVRVGSLKTVTLTDDGS
                     EQVSGYHLAGEIVGVEGVGNDVHACQAVALEDTEVCTLPFDRVEQLARDDASFQRRLY
                     RLLSSAIVRERTVALVVGTMSAEQRLASFLVDLSNRYHARGYSPTEFVLRMTREEIGS
                     HLGLKLETVSRIFSRFAEERLIVVSGRVIKLLDRAALHRLVAAS"
     gene            10519..11931
                     /gene="hemN"
                     /locus_tag="VFO53_15465"
     CDS             10519..11931
                     /gene="hemN"
                     /locus_tag="VFO53_15465"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET8877727.1"
                     /translation="MSANAALSASRSGLRFDPALVRKYDGFGPRYTSYPTADRFHAGF
                     TVADYADALAARNQRPAAQPLSLYVHLPFCNTICYYCACNKIITANRSRSAKYVRYVG
                     REIGIVSSLLEGARRVQQLHWGGGTPTFLAHEEMASLMKMLRDAFAFDANTEASIEVD
                     PRKVEAATVAFLSELGFNRISLGIQDFDPAVQQAVNRIQSEEETRAVIVAARAHGFTS
                     VNADLIYGLPRQTVHGFEATLDKVVDLSPDRIALYGYAHVPHLFKAQRQIVAHELPAA
                     DTKLAILSTAIDRLTKAGYVYIGMDHFAKPGDELAVALREGKLHRNFQGYSTRPDCDL
                     VAFGMSAIGKVGPAYVQNAKTLDAYYAALDEGRLPVHRGFELGDDDLLRREIIQKLMC
                     EFRVDVAALEAAHGFRFTERFARELTALAPLAADGLVTLSPTRIDVTPRGRMLVRTVA
                     MVFDRYLSEARERAAYSRVI"
     gene            complement(11960..13303)
                     /locus_tag="VFO53_15470"
     CDS             complement(11960..13303)
                     /locus_tag="VFO53_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8877728.1"
                     /translation="MTGYAEKTAQLLSAIASAQPAQGPVVGLSKATSNLFRDRARRSP
                     RIDLSHFNEVIAVDPAEHFVEAEGMTTFVDLADATLARGAMPAVVPQLKSITLGGAVA
                     GVGIEATSFRQGLVHDTVIAMDILTGDGRIVTCAPGNEHRDLFHGFPNSYGTLGYALK
                     LTARTRPVGRYVRVEHARHVDADRYFAALAQACSDDAVDFVDGVAFARDDLVLSRARF
                     VDDAPRVSDYTYEAVYYRSLRDKPVDYLTTRDYLWRWDTDWFWCSKNIGAQRPLVRTL
                     LGRERLNSITYQKIMRWNTRWHLTGALNRLRGVHSESVIQDVDIPIARAAEFLDFLHR
                     DVAILPIWICPIRAPVPGADALLYPLPRGTLSVNFGFWDTVSSRETRPAGFVNRKIER
                     KVQEVGGVKSLYADSYFTKDEFWSIYDGNAYRALKARYDPQGVLGDLYEKVVLRR"
BASE COUNT         2217 a         4442 c         4531 g         2270 t
ORIGIN      
        1 ccctgcggct ctcatgctcg gggtgcgcgt ccctatcacg tgcaaaggag cggaacatgg
       61 ccaggagggc tgcagcgact gccaaggctg acagcggtgc gcgcaagcag gcggcgcgca
      121 ggcgctcgcc gccgaagatg aacgcgaacg actcgccggg cgatcggtcg ccggcggaca
      181 agtcgtcgcc tgcccctgcg atccccgact tcgccgcgat ggcaaagatg gcgaagatga
      241 tgacgcccga gcaggcaatc cagttgtaca aggcgaatgc cgcgctcgcg ctcgaggtga
      301 tcaacacggc aatcgaaggc acggcgcggt tgcgcaggaa gcagctggaa ggtgctgccg
      361 aggcgagcga gttccagcac aggcacgcgc aatccgccgc gcacgcacgg gatccgcaag
      421 ccctcgttgc ggccgggcag ggtgccgcac aggaggcgat ggagcgatcg ctgcgctact
      481 ggagcgagat gttcgacctg atcgtcgaga tccagaagcg cctgttcacg ctcatggaag
      541 aacagatgga aggcgtgccg ggggtcaagg agacgcgcgc cgcgatgacg atgctgcccg
      601 acatggggca gatgcagaag gtcgtctccg cgatgcaggg cgtcgtgtcg tccggcgaat
      661 cgacgttcgc ctcgatgcag cgcgtgatgg gcgacttcgc gaagctcgcg caaggtggcg
      721 tgccgggcgg tcgccggtga gcgcggcaaa gcgtgccgaa cccggtgtga cggcgtcggg
      781 ccgcctcgca ccggcgatcg gcagatcgct gcgccacctg gtcccggttg caatcgcgct
      841 gctgctcgcc ggttgcgtgc cgatcggcgt gcgtgtgcag aacatgctgg ccctgtggca
      901 gggctgacgc ggagcgttac aggaaaagcc gggcgcggat tacaaaacgc tggtacgatc
      961 gtcccccatt cgagggagca ctttgggatg gatcgtcggc tcgactggat cgtacgcaag
     1021 ggtgcgctcg ccgcagccct ttacctcgcc atcgttggcg gcatcgactg gctgcagtac
     1081 ggcatcgccg catgcgtctg gtggccgctc gcaacgaatg tgtgggcggt tcccgtcgct
     1141 gcgccgtggc gatcggcggg gcccgcgccg ttgccaccga cggccgcaat gaccttcgat
     1201 ctcgcggtcc tcggctcgct gtttctcgct cactggtact ggacggcatt cgcctacgcc
     1261 atctcgtgcg gtatcgccgc actggtgcac gcgcgagcgg tcagcaagcc gtaaccggtt
     1321 cgcctgtcga ttggccggca gccgccagcc gccgtgacgg cgctttggcg gacgtcgttc
     1381 cgtgcgcgcc gcatacaggt ttggcacgga ccgtgcgtaa ggcgacgcca tgcaagcaaa
     1441 ggcattgcct cggccgcccg tttgtgcgac cccgtccgag aagcggggcg gtgtggcgcc
     1501 gcgagtttcc aaaggcatct ccctcttcga tgccctccgt cacgcgccag cccaccggcc
     1561 atcgctcgcg tggcttgcgc tcgtcctcgg atttgccgcg tcgttcggcg tcctgctctg
     1621 ggtgaacggc gtggtcgagc gcaaggcgca ggcagccttc gccgaggatg cgagcgacac
     1681 ggcaagcgag gtcgcccggc gtgcgcgcgc gtacgcggac gtcctgttcg cggtgcgcgg
     1741 cctcttcgac gtgtcgcaga acgtcacacg cgcccaattt caccgcttcg cgcgcggact
     1801 cgacttcgag cgtcgctatt ccggccttgc caacctctcg ttcgcatacc gggtgcgaag
     1861 cgacgaacgg aacgcattcg agcggcgaat gcgcaaggaa ggcggtgagc tgtcccagga
     1921 cctgccgccg ttcgccatca aggccagcga agcgcggccc gagcacatcg ttctgagcta
     1981 cgtcgatccg atcgaggaag gcagccaggt gcttggcttc gacctgtcgg acgatccggc
     2041 gcggcttgcg gccatcgaga gcgcgcgcga cagcggaggc ctgtcgacga cgccgtcgat
     2101 cgcgaagctg accgatccta aagttcaccc gtcgagcttc gtgctgaggc tcgctgtcta
     2161 cgatggcgga gtcatcccgc cgacgatcga ggcgcggcgc cgtgccttcg tcggcgtcgc
     2221 gggcgcgacc attcgtctgc ccgacgtgat caaggcgagc gagcaggcga ccgcgctcgg
     2281 caacatccgc gtacgcgtcg aggatgtcac gggccgtccg tccgtcacca aggggacgct
     2341 actgttcgac agcggcgcac cttccggaca gacgtcgccg atcctctacg ccaaatacgc
     2401 aaccgcgaac cggttcatca tgggcgaccg catcctctcg gtggcgatca cgccgcttgc
     2461 gaagccccag ccgcgcggcc tcgacgtttt cctgccgtac atcgcgggcg cgctgacgat
     2521 gatcggagcg ctgctcgtct ttgggctcat acgcgcgctg gccaaggccg aagtccgcgg
     2581 cgagtcgctg cgccgaagcc tgcgtgggct cgagttccat cgcgcgcggc tcgccgaaac
     2641 ccaggaaatc gccaatatcg gcggcttcga atgggacacc cgcgcaagcg cgcaggtctg
     2701 gtcggaccag ctctaccgaa tcctcggccg ggaagtcggt gacccgccgc accccgacca
     2761 tgattttttc tttgcgaacg ccgtgcacca cgccgatgcg ccggccgtgc gcgaggcact
     2821 gcgccgggtt ttgcacgatg gcggaccggt ggcactcgat tgccgcatcg ttcgccccga
     2881 cggcgccgag agaatcgtct cgacgctcac gcgtctcgaa gcggcagacg atggcgcgcg
     2941 aacgcgcgtc gtcggcaccg tgcgcgatat caccggcgag tggcgcgcag ccgagcgcga
     3001 gcgggcgcag cttcacttca ttcaaacggt gatcgatgcg attccgatcc ccgtctttca
     3061 gaaggacacc gaggggcgct ttcgcatctg caacgatgca tggtgccgct tcgtcggttt
     3121 gtcgcgcgag atgcttatcg gccgtaccgc ggaggaggtg ctgcccggca cggacttcga
     3181 cgcggttcgc gtccgggaca ccgagctcct tgcgcgcgtc ggtacggaca gcatcgaggt
     3241 cgatctgccc aacgccgccg gcaagctgcg ccgcgtcatg ctgcacaagg cgagctaccc
     3301 ggcgcgcgat ggagcgatcg ccgggctcgt cggtgcggcc ttcgacatca ctgaattgcg
     3361 ccagacggag gatgagctgg aacgcacgat cgcccagctc gaccgtcgca attgcacggc
     3421 gatgctgctc ggcgaattcg gcgaggtgct gcaggcctgt ctcgacctcg aggatgcgta
     3481 tgaggccgta gagaaatacc tgccgcgtct gttgccccgg agtgcgggta tcgtctaccg
     3541 agtgggcgcg gggcggggca acgccgagcg ttgcgcgacc tggggagcgc ctgtcggact
     3601 gaaggatgtg atcgcgacga gcgactgcgt cgcggttcgc cgggggcagc cgcgttgcgt
     3661 cgagcaatcc gaagaccagc tcaactgccg ccatttcacg gcgccgcctg catcttatgc
     3721 ctgcgtaccg ctatcgtctc acggggagct tctcggtctc ctgcacgtgc aggcgtccgc
     3781 cggctgcgcc ggcgcatgcg ataccgaagc cgaatggccg gcgcttcgca gcgcgtgcga
     3841 gcagatctcg ctcgcgctcg cgaacctcgc catccgcgag acgctgcgtg agcaggcgac
     3901 acgcgacaag ctgacgggtc tttacaatcg tcactacctg aacgcccgct tcgagcagga
     3961 ccttgcacgc gcgcaccgcg gcgaaagccc gattggcatc gtccttctcg atatcgatca
     4021 cttcaagcgc ttcaacgaca ccttcggcca cgccgcgggg gatcacgtgc tgcgggaggt
     4081 cgccgcggtc ctcatgagcg tgactcgccg ctcggatgtc gcttgccgtt acggcggcga
     4141 ggagttcctg ctgctcatgg ccgacgcctc gcgcgagatc gcgatccgcc gcgccgagga
     4201 gctgcggctc gcggtgaagg ggcttcgtct ggaatggcag ggacacccgc tgggtgagct
     4261 tacgatttcg gccggcgtcg ccgccttgcc ggccgatgga accgaggccg agatcctcgt
     4321 ccggcgcgcg gaccaggcgc tctaccgcgc caaggagctt ggccgtgatc gcgtcgaggc
     4381 cgcgctgcca ttcgccgcat tcgcgaacgg ttgaggcacc gagggacggc tacgcgaagc
     4441 gcgcaggccg gcgaattccg ccggatgctg ccaaagtttt agccacgtcg gcgagcacgg
     4501 tcaactgcgc gacgcaggcg attgaagcgc ctcgccttag gccggcgagc tgtggtcaac
     4561 gtgaaccgcc gtcgcttacc gcgttggaag cccgcgtaac ccatttgcac gtggtccaga
     4621 ctagccacga gtgcgcgcat gccgataagc cgcacgccct tgctgttggc cgccgccgaa
     4681 accggtttgt actgttcaca tcgtgagact ggacgcgtta cgatatatcg taatacgata
     4741 cgacatcggc gcgcgcgtac tccctctgcg cgacgacctt agccctcggc gcgtctccct
     4801 ggccgggggt ttttcttggg tcgagcgagc ggggtagggg atgacacggc ggcttctgat
     4861 tacgaattgc tttggcggtt gcggcatcgc aatcacctct cgtacgccgt acccccggcg
     4921 ttactccgaa cgcgaccccg atacccctac ggctgcaacc ggatgctgcc ggacggcgcc
     4981 ggtccgatgc acgatcgttt cataggggaa aagcgcgacg attgaatgct gtcggatgcg
     5041 accggatgct actttggtgc ccgggaccgg actcgaaccg gtacggcctc gcagccgagg
     5101 gattttaagt cccttgcgtc tacctgtttc gccacccggg caatgccgct gattgtagcc
     5161 gtcgccgcga gggaccgacg cctccgacgc gggtgtaaac tggccgctcg caccggtgca
     5221 cggaataggg cgggccggcc gcgtgtttgt cccgatcggg tttgccgtac ccagtctgcc
     5281 gaatcgccgg ccggcgtacc cgcgctgccg gcgcggcttt cggctttcat ccatccatga
     5341 agggtctacg atgaacgcgt ttctcaaatc gaaaagtctg atgcaggttt cgtgctggtc
     5401 ccttgccgcg gcgctggcgt gcacgtcggc tgcggcactc gccgcggcga tgaacgtcaa
     5461 gctttcgggc gccgaggaag ttccggccgt gcaaagcagc ggctcgggca gcggctccat
     5521 cacgatcggc gacgatggca gcgtgagcgg cagcgtgacg gccagcggat tcacgccgac
     5581 ggccgcgcac atccatgaag gcaaggcggg cgcgaacgga aaggtgatcg tgccgttcac
     5641 gaaggagggc gacaagttca gcgccccggc gggcgccaag ctgacgcccg accagatgaa
     5701 ggcgttcaag gagggcgacc tctacgtcaa catccacagc gccgcgcatc ccggtggcga
     5761 ggtccgcgcg caactcaagc cgtaacgcgc gtcgttctgt cacggccgcg tcccgatcgg
     5821 tgggacgcgg ccgttttcat gaagcgagtc gtgcgcgtcc ggcgatcccc ggtcagtgga
     5881 tcagcaccac cgggatgtgc gcgagatgca gcacgcgcgt cgtcaccgac cccatcgcca
     5941 tgttggcgag tgcggacatg ccgcgcgttc ccatgaagat catgtcggat cccgcttgct
     6001 tcgcgcgctc gacgatcgtt tcggcgacgg gcccgaccag acgctgcacc gtgtagggca
     6061 ccccgccggc gtcgagcagc gcgcggctcg gcctcagcat ggcgtcgctc tcgtcggcgt
     6121 agtatttctg caccatctcc ttgctgacga actggcccat gttcggcacg cgggggagcg
     6181 gaagatggac cgtgcaaagc tcgaccgcag gcgcctcgcg gtaccaggcg agcgtctcga
     6241 tcagcgttcg cgtcgcgttc aacgcgctcg ccgacccgtc gacgggcaac agcactttca
     6301 gcatcgcatc ctccggagga tgtgcacggc atttcgccag cgacgattgt cgcagatcgg
     6361 cggcttgccg gataatcgaa ctccgcgccg tttccgctga ctgattcgag ggtggaacca
     6421 gcgtccgggt atcgtgccgc tttgcggcgg caatgcacgg accgggtgta atgcacgacc
     6481 agactccaga cctcgacgcc ctcttcgacg acgcaccgaa acccgaccga acgttcgatc
     6541 cgtcggacgt cggccatctg ccgccgcccg cacaacgcta cctgaggcac gcgatcgcgc
     6601 ccgatacgcc gctcgcgtcg gcggtgcgcc tgaggatgca cggcgcgatc aggctcaaac
     6661 gctggcatcc gttcaaggcg gagcaggtga tcgtgcgcga tcgcgggatg atctggcgcg
     6721 cgcgggtgaa gctcgcgggc gcgaccatcc ggggattcga ccgcttcgtc gacggcgcgg
     6781 gcgccatgca gtggacgctg tgcggtctgc tgccggtcgt gcgtgcatcg ggccccgacg
     6841 tgacgcgctc gtcggcggga cgcttcgcgg ccgaatcggt ctggctgccg tccctgttct
     6901 gcaccgacca ggtgtcgtgg cgcgccgacg accacgatgg cgtcgcgcat gcacatctca
     6961 gtgtcgatgc gcacgacgag gatctcgagc tcacgctcaa tggcggatcg ctgcaatcgg
     7021 tcgcgttgcc gcgctggggc aatcccgacg gcggaaattt tcgcgaagtc gagttcggcg
     7081 ctgccgtcga tcaggaagcg accttcgggg gttacaccat tccggtgcgc ctgcgggtgg
     7141 gatggtattt cggcaccggc cgcttcgagg gcgagggcaa gttcttccac gcaacgatcg
     7201 acgatgccgt attccggtag gcgccagcgt gccggtgctc gcgcactctc tataatccgc
     7261 cgatgcgatc gcctgcccgc ggctcgattg ccgccgccca tgccctgctt gcgctcgccc
     7321 tcatcgcact cgcggcagcc ggctttctcg cgtcgttccg cgttgcatgg ctgattgcgg
     7381 caagcctgtg cgccgcggcc gcggcgctgg tcgcgctgtt cctgctgtcg gcggagacgc
     7441 gcgggcggcg gatggccgat ctcgagttgc ggcgaagcga gggacgcctt cgcggcatcc
     7501 tcgactcggc aatggattcg atcatcaccg tcgacgagaa ccagcgcgtc gtgctcttca
     7561 acgatgcggc agaagcgatg ttcggctggc ggcgcgacga agcgatgggt accgccctcg
     7621 acaacttcat ccccgagcgc tttcgccacg tgcatgccgc gcacgtcgag cgcttcggcg
     7681 aaacgcgcac cgcggcgcgg cgcatgggcg ggtcgctgcg aatcgtcacc ggattgcgtc
     7741 gcaacggcga cgaatttccc atcgacgcat cgatctcgca gatcgaggaa tcgggcaagc
     7801 gcttctacac ggtgatcctg cgcgacatca ctgcgcgcct cgcgtccgag gaagcgctgc
     7861 gtcgttccag ggatgacctg caggcgctgg gcgcggccgc acacatgacg cgcgagcagg
     7921 agaagagccg catcgcccgt gagctgcacg acgagctcgg gcagttgttg acgatgctgc
     7981 agatggacgt cgcctggtgc aaggaggcgc tgccttacgg ccacgaggcg ctcgagacgc
     8041 ggctcgaaag gatggaaaag ctgctcaaga gcaccatcgc ggcaacgcgc cgcatcgccg
     8101 ccgacctgcg gccgctgatg ctcgacgacc tcggtctcgt gccgtcgctc gagtggctgg
     8161 tcgagaactt ctcgcagcgc acgggcatcg catgcgcgct gtctgtcggc gagcgcgacc
     8221 ttgcattgcc gaaggcacat gcgaatgcga tctttcgcat cgtgcaggaa tcgctgacca
     8281 acattgcgaa gcatgccgac gcgtcgcgtg ccgagatcgc gctcgagcgc gacgaggatg
     8341 agctggtcgt gcgcgtcgcc gacgacggcg tcggcttttc gccgcaggca ccacgcaagc
     8401 ccggctcgct cggcctcttc ggcctgcgcg agcgggcgtc gctcatgggc ggtgaggcgt
     8461 cgatcgtcag tgcacccggc gaaggcacga ccatcgttgt gcggctgccg gtcgaagcgc
     8521 ccatggcgtc ggcatgattc gcgtcgtcat cgccgatgac caccagatcc tgcgcgaggg
     8581 actgaagcag cttctgcttg ccgccggcga tctcgacgtc gtgggcgacg ccggtgacgg
     8641 ccacgaagtg ctcgagcgcg tgcgcacgct cgggttcgac gtgctgctgc tcgacatgtc
     8701 catgccgggg aagagcggtg tcgagctcat caggcaggtc aaggcggaaa agccgaagct
     8761 gcgcatcctc gtgctcagca tgcacgagga gcatcaatac gcggtgcgtg cgatccgcgc
     8821 gggcgcgtcg ggatacctca cgaaggaaag tgctgcggca cagctcgtcg cggccatccg
     8881 caagattgcg gcgggaggcg ctttcatcag cgccgaagtc gccgagcggc tggcgctcga
     8941 cgcaatgccg cacgccgaag ggccgcttca tgcaacgctt tccgaccgcg aataccaggt
     9001 gttccggctg ctcgtcaccg gacgctcggt gagcgacatc gccgagcagt tgcacctgtc
     9061 cgccaagacg atcagcacgc acaaggcacg cctgatggag aagcttcagg tcgacaacaa
     9121 cgcggcactc gtgcgctacg cggtcgacca ccggctgcag gacgatccga acgctcccgc
     9181 gtaggcggtg gccgggtagg gcgtttccta cccggcatcg gtcagtgcgg acggcgtgaa
     9241 ccggttcgtc ccgacgctcg caatcagcgg ccaccgatgg ccgcgagccg gcatggatac
     9301 caataatggc gtccatgaac accccagcac gcacccacgt cttcatcgtc gacgacctcc
     9361 cgtcgatgcg cgagcggctg cgcgagctgg tggacggtgt cgacggcatc gaagtggtcg
     9421 gcgaggccgg cacgcccggc gaggcgatcg acggaattcg ccggacgcac cccgattacg
     9481 tgctgctcga ctatcagctg atcggcggca ccggcgtgga tgtgctccgc gcggttcatc
     9541 ccgatgcgcc cgacatcgcg ttcatcgtgc tcaccaacca cgccacggcg cagcaccgcc
     9601 gcgcgtgtct cgacgccggt gcgcgcttct ttttcgacaa gagtaccgag ttcggccgcg
     9661 tgtgccgcgc aatcgccgaa agccgacccc cccgcccctg aggatatcga tgctagtctc
     9721 caccgccgtt cgtgttcggg aaccgtcggg gccgcagccg gcggtcaccg cgcggccgct
     9781 gcttcgcgac gtgagccgcg atgtcgcctc gtgcacggtc tgcacgctgc ggcgaatctg
     9841 catgccggtc gatatccagg atgcggaggc gcggaagctg ttcgagcggc tcgtcaccac
     9901 gcgaattcgc ctgcgcaagg gtgacgtgct gtttcgcgcc ggcgaccgct tcacggcgct
     9961 cttcgcggtc cgcgtcgggt cgttgaagac ggtcacgctg accgacgacg gcagcgagca
    10021 ggtgtccgga taccacctgg cgggcgagat cgtcggtgtc gagggcgtcg gaaacgacgt
    10081 gcacgcgtgc caggcggttg cgctcgaaga taccgaggtg tgcacgctgc ccttcgaccg
    10141 ggtcgagcaa ctggcccgcg acgacgcatc gttccagcgc cggctgtatc ggctgctgtc
    10201 gtcggcaatc gtgcgcgagc gcaccgttgc gctggtggtc gggacgatga gtgccgagca
    10261 gcggcttgcg tcgttcctcg tcgacctgtc gaatcgctac cacgcgcgcg gctattcacc
    10321 gaccgaattc gtgctgcgga tgacgcgcga ggagatcgga agtcacctcg gactcaagct
    10381 cgagacggtg agccggatct tctcgcgctt tgccgaggaa cgactgatcg tcgtaagcgg
    10441 ccgcgtgatc aagctcctcg accgggccgc gctgcatcgg ctcgtcgccg cgagttgact
    10501 cttcgccgca acctccgcat gagcgcaaac gccgcacttt ccgcatcgcg aagcgggctt
    10561 cgcttcgatc ctgcactcgt tcgcaaatac gacggtttcg ggccgcgtta cacgtcgtac
    10621 cccacggccg atcgcttcca cgccggcttc accgtggcgg attacgccga cgcgcttgcc
    10681 gcccgcaacc agcgtccggc ggcgcaaccg ttgtcgctct acgtgcatct gccgttctgc
    10741 aacacgattt gctattactg cgcgtgcaac aagatcatca cggccaaccg cagccgctcg
    10801 gcgaaatacg tgcgctacgt cggccgggag atcggcatcg tctcgtcgct gctcgaaggc
    10861 gcgcggcgcg tccagcagct gcattggggc ggcgggacgc cgacgttcct tgcgcatgag
    10921 gaaatggcat cgctgatgaa gatgctgcgc gacgcgttcg cgttcgatgc gaacaccgag
    10981 gcgtcgatcg aagtcgatcc acgcaaggtc gaggcagcga cggtcgcgtt cctgagcgag
    11041 ctcggcttca accggatctc gctcgggatc caggatttcg acccggcggt gcagcaggcg
    11101 gtcaatcgca tccagagcga ggaggagacg cgggcggtca tcgtggcggc acgcgcgcac
    11161 gggttcacgt ccgtcaacgc cgacctgatc tacgggctgc cgcgccagac cgtccatgga
    11221 ttcgaagcga cgctcgacaa ggtggtcgac ctttcgcccg accgaatagc gctctacggc
    11281 tacgcgcacg tgccccatct tttcaaggcg cagcgccaga tcgtcgcgca cgagctgccg
    11341 gcggccgaca cgaagctcgc aatcctgtcg acggcaatcg acagactcac gaaagccggg
    11401 tacgtgtata tcggcatgga tcatttcgcg aaacccggcg acgagctcgc cgtggcgctg
    11461 cgcgaaggca agctgcaccg aaacttccag ggctactcga cgcgccccga ctgcgacctg
    11521 gtcgcgttcg gcatgtcggc gatcggcaag gtcgggccgg catacgtgca gaacgcgaag
    11581 acgctcgacg cctattacgc cgcgctcgac gaaggccggc ttcccgtgca tcgtggcttc
    11641 gagctcggcg acgacgacct gctgcgccgc gagatcatcc agaagctcat gtgcgagttc
    11701 cgcgtcgacg tcgcggcgct cgaggctgcg catggctttc gcttcaccga gcgctttgcg
    11761 cgtgagctca cggccctcgc gccgctcgcc gcggatggcc tcgtaacgct gtcgccaacg
    11821 cgtatcgacg tgacgccgcg cgggcgcatg ctcgtgcgca ccgtggcgat ggtgttcgac
    11881 cgttacctga gcgaagcacg cgaaagggca gcgtactcgc gcgtgatctg aagcgctcgc
    11941 tcgttcgtgg tgcgcgacgc tatcgccgca gcacgacctt ctcgtagaga tcgccgagca
    12001 ccccttgcgg gtcgtagcgc gccttcagcg cacggtaggc gttgccgtcg tagatcgacc
    12061 agaattcgtc cttcgtgaaa tacgagtcgg cgtagagcga cttcacgccg ccgacttcct
    12121 gcaccttgcg ctcgatcttg cggttgacga agccggccgg ccgcgtctcg cgcgacgaaa
    12181 ccgtgtccca gaagccgaaa ttgaccgaca gcgttccccg cggcagcgga tagagcaacg
    12241 cgtcggcccc cggcacgggt gcgcgaatgg gacaaatcca gatcggcagg atcgcgacgt
    12301 cgcgatgcag gaagtcgagg aactcggcgg cacgggcaat cggaatgtcg acgtcctgga
    12361 tgaccgattc cgaatgcacg ccgcgtaagc ggttgagcgc gccggtgaga tgccagcgcg
    12421 tgttccagcg catgatcttc tggtaagtga tcgaattgag gcgctcgcgc ccgagcagcg
    12481 tgcgcacgag cggccgctgc gcaccgatgt tcttcgagca ccagaaccag tcggtgtccc
    12541 agcgccagag gtagtcgcgc gtcgtcaggt aatccaccgg cttgtcgcgc agcgaccggt
    12601 agtagacagc ctcgtacgtg tagtcgctga cccgcggcgc gtcgtcgacg aagcgtgcgc
    12661 gggacaggac gagatcgtcg cgcgcgaacg caacgccgtc gacgaagtcg acggcgtcat
    12721 cgctgcatgc ctgcgcgaga gcggcgaagt agcggtcggc atccacgtgg cgcgcgtgct
    12781 ccacgcgcac atatcgcccg accggccgcg tgcgggcggt aagcttcagc gcgtacccca
    12841 gcgtgccgta cgaattgggg aaaccgtgga agaggtcgcg atgctcgttg ccgggcgcgc
    12901 aggtgacgat gcgtccatcg ccggtcagga tgtccatggc gatgaccgtg tcgtgcacga
    12961 gcccttgccg gaacgacgtc gcctcgatgc cgacgccggc gacggcgccg ccgagcgtga
    13021 tcgacttcaa ctgcggcacg actgccggca tcgcgccccg ggccagtgtc gcatcggcga
    13081 ggtcgacgaa cgtcgtcatc ccttccgctt cgacgaaatg ctccgccggg tcgacggcga
    13141 tcacttcgtt gaaatggctg aggtcgatgc gtggcgagcg tcgcgcgcgg tcgcgaaaca
    13201 gattcgacgt cgccttggaa aggccgacga ccgggccttg cgcgggttgc gccgacgcga
    13261 tcgcggacag gagttgcgcg gtcttctctg cgtaacccgt cacgtggcgg gcgggtacag
    13321 gaacgcgcgg ctcatcgggt aatccgtgac gttccccttg ctcaccgtca cctggaagag
    13381 gtgggtgcgc gacgtgcgcg agcggaacat ttccgcgcac gaccagaggt acgtgcgcca
    13441 gatccggcga tgacgctcgt
//
LOCUS       DASSVX010000364         9595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_965874, whole genome shotgun sequence.
ACCESSION   DASSVX010000364 DASSVX010000000
VERSION     DASSVX010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965874"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="VFO53_15475"
     CDS             <1..1482
                     /locus_tag="VFO53_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8877729.1"
                     /translation="LAQYMSQGLCRVKWLSAAAILVWVLQAVALVQGAMGYLYRPAAE
                     RLSVSLPTLIFGCVAIGIAGAAGILASGRRGITVARARALVLTMALTTVVSESLLFGF
                     SRAAPERIASLDLTLLLVFAIMPLGALSAIFVFVVAVLVVVASAVAAGHVFSPEHAAF
                     ILHPMSFAALGFVANRWYVGSFRGEQLGRQRLQRHMRQVERQKQLIEAQKDESERQRA
                     EITRQRSVLFQALSSALTAPVAHAYVEHGHFPTELKTVCVIACDAVGFSDTCRKLQPE
                     RVVFELENFFREFDGACLKYRLEPLRAQGDSRIAIAGLWPGANRHLQQEAISAVLAML
                     YFRDALPGVVTGAEASRGRVLWQARMGICLGPASCGVIDTGVTDGVNSTGRLWFDVWG
                     DTVNLAARIQEAAQPNQLLVRESVLWETCGLFDHGPIRQVHVKSTTLSDVAEITGIRA
                     GFRNEAGMPNAAFWDVFNDPSVRPVRPNPAGTSVDNALEMRTP"
     gene            1529..2845
                     /locus_tag="VFO53_15480"
     CDS             1529..2845
                     /locus_tag="VFO53_15480"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET8877730.1"
                     /translation="MRPLHVGLVGFGTVGRGTWDVLRRNEEEITRRAGRPIRITWIGT
                     RSLERARQGMRGITGVQFSDDPAAVVRHADVDIVCELIGGIEPARSLILDAIAHGKHV
                     VTANKALLAQHGNEIFAAAHAKGVMVAFEGAVAGGVPIIKALREGLTANRIEWIAGII
                     NGTSNFILSTMRETGGSFAAALAQAQARGYAEADPAFDVEGTDAAHKLTIMSAIAFGV
                     PMQFDKAYAEGITQLTREDLRYAEELGYRIKLLGITRRARSGIELRVHPTLIPAKRLI
                     ANVEGAMNAVLVKGDAVGPTLYYGAGAGAEPTASAVIADLVDVTRMHTADPEHRVPHL
                     AFQPDQLTDVPFLPIGDVETSYYLRMRVLDRPGVLADITRILADRDISIDAMIQKEPT
                     EGEDQTDIILLTHRSIEHRVVDAIARIESLPTVRGTVVRIRLEDLA"
     gene            2860..4278
                     /gene="thrC"
                     /locus_tag="VFO53_15485"
     CDS             2860..4278
                     /gene="thrC"
                     /locus_tag="VFO53_15485"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET8877731.1"
                     /translation="MNYVSTRGAWNDAPQPFSAILLEGLAPDGGLAVPQAYPRLAADE
                     LANLRRLSYPDLALAILDLFIGDIPTAELRDIVRRTYDAATFGSEDITPLSTLEPGLH
                     LLHLSNGPTLAFKDIALQLLGNLFEYALRDSGRRLNILGATSGDTGSSAEHAMRGKRR
                     LAVFMLSPRGRMSAFQQAQMYALRDPNIHNLAIEGTFDQCQDIVKALSGDRAFKARHS
                     LGAVNSINWARIAAQVVYYFKGYFAATARDGERVSFAVPSGNFGNVLAGHVARQMGLP
                     IARLVVATNENDVLNEFFLTGRYRPRAAAETHATSSPSMDISKASNFERYLFDIAGRD
                     AEQLALLWQRLERDGSFDVAASSLWPRVEASGFVAGSSTHAERIATIRDVHAHCGVLI
                     DPHTADGVKVGRGFRESGVPMICLETALPAKFAATIREALGIDPPRPAAYAGLESRPQ
                     HCTVLPADAARVAAFIDAHAGA"
     gene            4291..5673
                     /locus_tag="VFO53_15490"
     CDS             4291..5673
                     /locus_tag="VFO53_15490"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HET8877732.1"
                     /translation="MRNALNALGRNLVAGLRVALFMPVERGAFQISAAQLVLIVLISA
                     AIDIDADWVRAPHDARFSVLGLHGELFALGLLTLSSALIATLRRERDVYLALPIVVLA
                     SFPAIQIVHLLPSLPGAAAAVSDVTRTVFEYAVLAWMLIIAVRAVYVCIDPQRRRRRV
                     FAVGAGVLLIAPLWFAPFAGPLDPWWSEFDSVQSNRDAMSPASEPVLAAQDFMMDRAL
                     DQLDDERPGVSDLYFVGFAPDARRPGFVTDVDAAQRAMDERWHTKGRSVVLVNSPLTV
                     ADRPFATITHLREVLLQIGDLIDADDDVVMVYLTGSSGADHSLTAVNPPLELVGLSPQ
                     GLKQLFDAAGIRWRIVVVSTCYAGAWADALKDDETAVIASSAKDVRGNDCAGGIATSA
                     FGEAFFTEGMRRNDDLRVAFDVARKGLARSHAPEPVMSIGPAIAEHLKRLRNQGGARI
                     VADAAVRERR"
     gene            5763..6761
                     /locus_tag="VFO53_15495"
     CDS             5763..6761
                     /locus_tag="VFO53_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8877733.1"
                     /translation="MAFALALVVVGAPAGAQGLEKTKITIGVGGKSLIYYLPLTIAER
                     KGYFKQEGLDVEIPDFPGGAKALQALLGGSVDIVSGAYEHTISQQAKGNAIEALVLEG
                     RYSGIVLALSKAKAASYKSPADLKGMKVGVTAPGSSTNMFVNILLAKAGLPPDSVSII
                     GVGGGAGAVAIMKRGEIDALSNLDPVIAQLVSDGTVTPVVDTRTAKGMQEVYGGAYAA
                     GCIYAPPEWVKKNPHTAQAVVNAMVRALRFIQNSPIDEVVAAVPPEYYTDKALYKKAL
                     EDNIATFRHDGFISKEAGEHVAHDLLTFDPTMKGKTIDLAKTVDMSFQQKAAQSIK"
     gene            6761..7594
                     /locus_tag="VFO53_15500"
     CDS             6761..7594
                     /locus_tag="VFO53_15500"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8877734.1"
                     /translation="MPSVAGGTTRAATAVLAFERVTCAFAGKTPGERYTAVADASLAI
                     VEGEFVSVVGPTGCGKSTLLNVAAGLLAPSSGRALVAGEPLAGINARAGYMFQADALM
                     PWRNALDNVTAGLEFRGVRREAAAARGREWLARVGLAGFEHRYPHQLSGGMRKRVALA
                     QMLILDPQILLMDEPFSALDVQTRQLMENELLELWSANRKSVLFITHDLEEAIALSDR
                     VVVLSAGPATHPIGEFPIDLPRPRDVAEIRLSKHFVELHEAIWHVLKSEVLKGYAQGK
                     R"
     gene            7611..8399
                     /locus_tag="VFO53_15505"
     CDS             7611..8399
                     /locus_tag="VFO53_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8877735.1"
                     /translation="MGLALWHVAILAIVFGVWYALTTPGLVDEAYANKIAFFFGRPLQ
                     VVNVVIAWFTSGKIYPHLAITLWETVLAFAIGSIFGLVVGLWLALSPTASAIAEPYIT
                     ALNAMPRVILAPIFAVWFGLGVLSKVALGVTLVFFIVFFNVYQGVKEVSPVVLANARM
                     LGASRKQLLRAIYLPSATSWVFSSLHTSVGMAFVGAVVGEYLGSAKGVGYLILQAEGV
                     FDINTVIAGIVVLTVFALLLDFMVTKIEARLLVWRPRTAETEAL"
     gene            8428..9054
                     /locus_tag="VFO53_15510"
     CDS             8428..9054
                     /locus_tag="VFO53_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HET8877736.1"
                     /translation="MTVPGDRISLAQWRRTIAELYAEVRAISATDPQHAAERFRAGRD
                     RLFRAHPDSPIPARDRAQWPGASWYRYDAAWRTIGVVKPLARTDTFDIALAADGVTRC
                     TRVATVEFTVRANAAMLTLYWFEGYGGGLWLPFGDASNAASTYGGGRYLYDTIKGADL
                     GASQNAFVLDFNFAYNPSCAYDERWSCPLPPSENRLPFEVTAGERTSR"
     gene            9157..9324
                     /locus_tag="VFO53_15515"
     CDS             9157..9324
                     /locus_tag="VFO53_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877737.1"
                     /translation="MCYEFESWHWKARAKEMHKAQVRTDAAEQKSVPAKSAKDTQRAR
                     PEVKEPEKVPV"
     gene            9371..9447
                     /locus_tag="VFO53_15520"
     tRNA            9371..9447
                     /locus_tag="VFO53_15520"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9405..9407,aa:Met,seq:cat)
BASE COUNT         1553 a         3138 c         3220 g         1684 t
ORIGIN      
        1 ctggcccaat acatgagtca gggattgtgc cgcgtcaaat ggctgtcggc cgccgccatt
       61 ctcgtctggg ttctgcaggc cgtggcgctg gtgcagggcg cgatgggcta cctctatcgc
      121 ccggcggccg aacggctgtc cgtttccctg ccgacgctga tctttggttg cgttgccatc
      181 ggcatcgccg gcgcggcagg cattctcgcg agcggacgca ggggcataac ggttgcacgc
      241 gcgcgtgcgc tcgtgctgac gatggcgctt acgacggtcg tgtcggagtc actgcttttc
      301 ggattctcgc gcgccgcacc cgagcgcatt gcgtcgctcg acctcacgct gctgctggtc
      361 ttcgcgatca tgccgctcgg tgcgctctcg gcgattttcg tttttgtggt cgccgtactc
      421 gtcgtcgtcg cgtcggccgt cgccgccggt cacgtattca gccccgagca cgcagcattc
      481 atcctgcatc cgatgtcctt tgcggcactg ggattcgtcg cgaaccgctg gtatgtcggc
      541 tccttcaggg gcgaacagct cggccggcag cgcttgcagc gccacatgcg ccaggtcgag
      601 cgccagaagc agttgatcga ggcgcagaag gacgagtcgg agcggcagcg tgccgaaatc
      661 acccggcagc gcagtgtgct gttccaggcg ctgtcgagcg cactgaccgc gccggtcgcg
      721 cacgcctatg tcgagcatgg acattttcca accgagctca agacggtgtg cgtcatcgcg
      781 tgcgatgcgg tcggcttcag cgacacgtgc cggaagctgc agccggagcg cgtggtgttc
      841 gagctcgaaa atttctttcg cgagttcgac ggcgcctgcc tcaagtaccg actcgaaccg
      901 ctgcgcgcgc agggcgacag ccgcatcgcg atcgccggcc tgtggccagg cgccaaccgg
      961 catttgcagc aggaggcgat cagcgccgtg ctcgcgatgc tttatttccg cgacgcactg
     1021 cccggcgtcg tcaccggcgc cgaagcttcg cgcggccgcg tgctgtggca ggcgcgaatg
     1081 ggcatctgcc tcggacccgc ctcctgcggc gtgatcgaca ccggcgttac cgacggcgtc
     1141 aactcgaccg ggcgtctctg gttcgacgtc tggggcgaca ccgtcaatct cgctgcccgc
     1201 attcaggaag ccgcgcagcc caaccagttg ctcgtgcgcg aatcggtact atgggagact
     1261 tgcgggctgt tcgatcacgg gccgatccgg caggtgcacg tgaagtcgac gacgctctcc
     1321 gatgtcgcgg agatcaccgg catccgtgca gggttccgca acgaagccgg gatgcccaat
     1381 gcggcgttct gggacgtctt caacgacccg tcggttcgtc ccgttcggcc gaatcccgca
     1441 ggaacgtccg tcgacaatgc actggaaatg cgcacgccat gacgatgatt gaagtgccat
     1501 cgcaaagtcg ctgccgccgt cggccgcgat gaggccgctc cacgtcggcc tcgtcggctt
     1561 cggcaccgtg gggcgcggca cgtgggacgt cctgcgccgc aacgaggagg agatcacgcg
     1621 gcgcgcgggg cgtcccatcc gcatcacgtg gatcggcacc cggagcctcg agcgcgcacg
     1681 ccaggggatg cgcggcatca ccggcgtgca gttcagcgac gatcccgccg ctgttgtccg
     1741 ccacgcagac gtcgacatcg tctgcgagct gatcggcggc atcgagccgg cgcgctcgct
     1801 gatcctcgac gcgatcgcgc acggcaagca cgtcgtgacc gcgaacaagg cgctgctcgc
     1861 gcagcatggc aacgagatct ttgccgctgc ccacgccaag ggcgtgatgg tcgcgttcga
     1921 aggcgccgtc gcgggcggcg tgccgatcat caaggcgctg cgcgaagggc tcacggcgaa
     1981 tcgcatcgag tggatcgcgg gcatcatcaa cggcacctcc aatttcatcc tgtcgacgat
     2041 gcgcgaaacc ggcggatcgt tcgccgccgc acttgcgcaa gcgcaggcgc gcgggtacgc
     2101 cgaggccgat ccggcgttcg acgtcgaagg aaccgatgcg gcgcacaagc tcacgatcat
     2161 gtcggcgatc gccttcggcg tgccgatgca gttcgacaag gcgtacgccg aaggcatcac
     2221 gcagctcacg cgcgaggacc tgcgttacgc ggaggagctc ggctaccgca tcaagctgct
     2281 cggcatcacg cggcgcgcac gaagcggcat cgagctgcgc gtccatccga cgctgattcc
     2341 cgcgaagcgg ctgatcgcca atgtcgaggg cgcgatgaac gcggtgctcg tcaagggcga
     2401 cgccgtgggc ccgacgctct actacggcgc gggtgccggc gccgagccga cggcgagtgc
     2461 cgtgatcgcc gatctcgtcg acgtcacgcg gatgcacacg gccgatcccg agcatcgcgt
     2521 gccgcatctg gccttccagc ccgaccagct caccgacgtg ccgttcctgc cgatcggcga
     2581 cgtcgagacg agctactacc tgcgcatgcg cgtgctcgac cgccccggtg tgctcgccga
     2641 catcacgcgc atcctcgccg atcgcgacat ctcgatcgac gcgatgatcc agaaggagcc
     2701 gaccgagggc gaggaccaga ctgacatcat cctgctcacg caccgctcga tcgagcatcg
     2761 ggtggtcgat gcgatcgcgc gcatcgaatc gctgcccacg gtgcgcggca ccgttgtgcg
     2821 cattcgcctc gaggaccttg catgacgggg catgcgatcg tgaattacgt cagcacgcgc
     2881 ggcgcgtgga acgatgcgcc tcagccgttc tcggcgatcc tgctcgaagg actcgcgccc
     2941 gatggcggcc tcgcggtgcc gcaggcttac ccgcggcttg ctgccgacga gttggcgaac
     3001 cttcgccggt tgtcgtatcc cgacctggca ctcgcgatcc tcgatctttt catcggtgac
     3061 attccgaccg ccgagttgcg cgacatcgtt cgccgcacct acgacgccgc aacgttcggc
     3121 agcgaggaca tcacaccgct gtcgacgctc gagccggggt tgcacctgct gcacctttcg
     3181 aacgggccga cgctcgcgtt caaggacatt gcacttcagc tcctcggcaa cctgttcgag
     3241 tacgcactgc gcgattccgg gcgccgcctc aacatcctcg gcgccacgtc gggcgacacc
     3301 ggcagctcgg ccgagcatgc gatgcgcggc aagcggcgcc tcgccgtttt catgctgtcg
     3361 ccgcgcggac gcatgagcgc gttccagcag gcgcagatgt acgcgctgcg cgatcccaac
     3421 atccacaacc tggcgatcga aggcacgttc gaccagtgcc aggacatcgt caaggcgcta
     3481 tcgggtgatc gcgccttcaa ggcgcggcac tcgctgggag cggtcaattc gatcaattgg
     3541 gcacgaatcg ccgcgcaggt cgtctattac ttcaagggct acttcgcggc gaccgcgcgc
     3601 gacggcgagc gcgtgagctt cgccgtaccg tcgggtaatt tcggcaacgt gctggcaggc
     3661 catgtcgcac ggcagatggg actgcccatc gcgcgcctcg tcgtcgccac caacgaaaac
     3721 gacgtgctga acgagttctt cctgaccggc cgctatcggc cgcgcgcggc ggccgaaacg
     3781 cacgcaacat cgtcgccgtc gatggacatt tcgaaggcgt ccaatttcga gcgctacctg
     3841 ttcgatatcg cgggacgcga tgcggagcaa ctcgcgctgc tctggcagag gctcgagcgc
     3901 gacggcagct tcgacgtcgc ggccagctcg ctctggccgc gtgtcgaggc gtccggattc
     3961 gttgcgggca gcagcacgca cgccgagcgc atcgcgacca ttcgcgacgt gcacgcgcat
     4021 tgcggcgtgc tgatcgatcc gcatacggcc gacggcgtca aggtcgggcg aggcttccgt
     4081 gaatcgggtg tcccgatgat ctgcctcgaa accgcgctgc ccgccaaatt tgcggcgacg
     4141 atccgcgagg cgctcggcat cgacccgccg cgcccggcgg cgtacgccgg actcgaatcg
     4201 cggccgcaac actgcacggt cctgcctgcc gatgccgcgc gcgtggcggc cttcatcgac
     4261 gcgcacgcgg gcgcctgatt cgccattgac atgcgcaacg cgttgaacgc cctcgggcgc
     4321 aatctcgtcg cgggcctgcg cgtcgcgctt ttcatgcccg tcgagcgcgg cgcatttcag
     4381 atctcggcag cgcagctcgt cctgatcgtc ctcatctcgg cggccatcga catcgacgcc
     4441 gactgggtgc gcgcgccgca cgacgcccgc ttctcggtgc tcgggctgca cggcgagctt
     4501 ttcgcgctcg ggctcctgac gctgtcgagc gcactgatcg cgacgctgcg tcgcgagcgc
     4561 gatgtctacc tcgcgctgcc gatcgtcgtc ctcgcgtcgt tccccgccat ccagatcgtg
     4621 cacctgctgc cgagcctgcc cggagcggct gccgccgttt ccgacgtcac gcgcacggtg
     4681 ttcgaatatg cggttcttgc gtggatgctg atcatcgccg tgcgcgcggt gtacgtgtgc
     4741 atcgatccgc agcgccggcg ccggcgtgtc ttcgccgttg gcgccggcgt gctcctcatc
     4801 gcgccgctgt ggttcgcgcc attcgccggg ccgctcgatc cgtggtggag cgaattcgac
     4861 tcggtgcaga gcaatcgcga cgcaatgagt cccgcctccg agcccgtgct tgccgcacag
     4921 gatttcatga tggaccgcgc gctcgaccag ctcgacgacg aacgtccggg cgtgtccgat
     4981 ctctacttcg tcggttttgc tcccgatgca cgccggccgg ggttcgtgac cgacgtcgac
     5041 gcggcgcagc gtgcaatgga cgagcgctgg cacacgaagg gacgctcggt ggtcctcgtc
     5101 aacagtccgc tgacggttgc cgatcggccg tttgcgacga tcacccatct gcgggaagtg
     5161 ctgctgcaga tcggcgacct gatcgacgcg gacgacgacg tcgtcatggt ctacctgacc
     5221 ggcagcagcg gcgccgacca ttcgcttacg gcggtcaatc cgccgctcga gctcgtgggc
     5281 ctgtcgcccc agggactcaa gcaattgttc gacgccgcgg gtatccggtg gcgcatcgtg
     5341 gtcgtgtcga cgtgctatgc cggcgcgtgg gccgatgcgc tgaaggacga cgagacagcg
     5401 gtgatcgcct cgtccgcgaa ggacgtgcgc ggcaacgatt gcgcgggcgg cattgcaacg
     5461 tccgcgttcg gcgaggcgtt ctttaccgaa gggatgcgcc gcaacgacga tctgcgagtc
     5521 gcgttcgacg tcgcgcgcaa agggcttgca cgcagtcatg cgccggaacc ggtcatgtcg
     5581 atcggtccgg cgatcgccga gcatctgaag cggctgcgca accagggcgg cgcgcggatc
     5641 gtcgccgacg cagcggtgcg cgagcggcga taatgcgcgt cgcgagacgc atttcgctgc
     5701 catccgttcg accccgtttc ataagcaata tggaggcata catgcggcat tgggcaggtc
     5761 tgttggcttt tgcactggcg cttgtcgtcg tcggcgcgcc ggccggcgcg cagggcctcg
     5821 agaagacgaa gatcacgatc ggcgtgggcg gcaagtcgct gatctactac ctgccgctca
     5881 cgatcgccga gcgcaagggc tacttcaagc aggagggtct cgacgtcgag attcccgact
     5941 tcccgggtgg cgcgaaggcg ctgcaggcgc tgctcggggg cagcgtggac atcgtgtccg
     6001 gcgcgtacga gcatacgatc tcgcagcagg ccaagggcaa cgcgatcgaa gcgctggtgc
     6061 tcgagggccg gtactcgggc atcgtcctcg cgctgtcgaa ggcgaaggcc gcgtcgtaca
     6121 aatcgccggc cgatctcaag ggaatgaagg tcggcgtgac ggcgcccggc tcgtcgacga
     6181 acatgttcgt caacatcctg ctcgccaagg cgggactgcc gccggactcg gtgtccatca
     6241 tcggtgtcgg cggcggcgcc ggtgccgtcg caatcatgaa gcgcggcgag atcgacgcgc
     6301 tgtcgaacct cgatccggtc atcgcgcagc tcgtcagcga cggcacggtg acgccggtcg
     6361 tcgatacgcg aaccgcgaag ggcatgcagg aggtttacgg cggcgcctac gcggcggggt
     6421 gcatctatgc gccgccggaa tgggtgaaga agaatccgca taccgcacag gccgtggtga
     6481 atgcgatggt gcgggcgctg cgcttcatcc agaactcgcc gatcgacgag gtggtggccg
     6541 ccgtcccgcc cgagtactac accgacaagg cgctgtacaa gaaggcgctc gaggacaaca
     6601 ttgcgacgtt caggcacgac ggattcatct cgaaggaggc cggcgagcac gtcgcgcacg
     6661 atcttctgac attcgacccc acgatgaagg gcaagacgat cgatcttgcg aagacggtcg
     6721 acatgtcgtt ccagcagaaa gcggcgcaga gcatcaagtg atgcccagcg tcgcgggcgg
     6781 cacgacgcgc gcggcgacgg cggtgctcgc gttcgagcgg gtcacctgcg ccttcgccgg
     6841 caagacgccg ggcgagcgtt acacggcggt cgccgacgca tcgctcgcca tcgtcgaggg
     6901 cgagttcgtt tcggtcgtcg gacccaccgg ctgcggcaag tcgacgctgc tcaatgtcgc
     6961 ggccggcctg cttgcgccat cctcgggtcg cgcattggtg gcgggcgagc cgctcgcagg
     7021 catcaatgcg cgcgccggct acatgttcca ggccgatgcg ctgatgccgt ggcgcaacgc
     7081 cctcgacaac gtgacggccg ggctcgaatt ccgcggtgtg cggcgcgaag cggctgcggc
     7141 gcgcgggcgc gagtggctcg cgcgcgtcgg gctcgccggc ttcgagcatc gctatccgca
     7201 ccagctctcg ggcggcatgc gcaagcgcgt cgcgctcgcg cagatgctga tcctcgatcc
     7261 gcagatcctg ctgatggacg agccgttctc tgcactcgac gtccagacac ggcaactaat
     7321 ggaaaacgag ctgctcgagc tgtggtcggc aaaccggaag tcggtgctct tcatcacgca
     7381 cgatctcgag gaagcgatcg cgctgtccga tcgcgtcgtc gtgctgtcgg ccggccccgc
     7441 gacgcatcca atcggagaat tcccgatcga tctgccgcgc ccgcgtgacg tcgcggagat
     7501 tcgcctgtcg aagcactttg tcgagttgca cgaggcgatc tggcacgtgc tgaaatcgga
     7561 ggtgctgaag ggctatgcgc aaggcaagcg gtgacgcgcg ccttggccgg atgggcctcg
     7621 cgctgtggca tgtcgcgatc ctcgcgattg tcttcggcgt ctggtacgcc ctgacgacgc
     7681 cgggcctcgt cgacgaagcc tatgccaaca aaattgcgtt cttcttcggg cgtccgctgc
     7741 aagtggtaaa cgtggtcatc gcatggttca cgtccggaaa gatttatcca cacctcgcca
     7801 tcacgctgtg ggagacggtg ctggcgttcg cgatcggcag catcttcggc ctcgtcgtcg
     7861 ggctgtggct cgcgctgtcg ccgaccgcgt cggcgattgc cgagccgtac atcaccgcac
     7921 tcaacgcgat gccgcgcgtg atcctcgcgc cgatcttcgc cgtgtggttc gggctcggcg
     7981 tgctgtcgaa agtggcactc ggcgtgacgc tcgtgttctt catcgttttc ttcaacgtct
     8041 accagggcgt gaaggaagta agcccggtgg tgctcgccaa cgcgcgcatg ctcggcgcat
     8101 cgcgcaagca gctccttcgc gcgatctacc tgccatcggc gacgtcgtgg gtcttctcga
     8161 gcctgcatac atcggtcggc atggcgttcg tcggcgctgt cgtcggtgag tatctcggat
     8221 ccgcgaaggg cgtcggctat ctgatcctgc aggcggaggg cgtgttcgac atcaacacgg
     8281 tgatcgcggg catcgtggtt ctgaccgtgt tcgcgttgct gctcgacttc atggtcacga
     8341 agatcgaggc gcgactgctc gtctggcggc cgcgcacggc agagaccgag gcgttgtgat
     8401 tgcgcgcagg aaccagggga gcatcgcatg accgtacccg gggatcgcat atcgctcgcg
     8461 caatggaggc gcacgatcgc cgagctgtat gcggaggtgc gtgcgatttc cgcaaccgat
     8521 ccgcagcacg ccgccgagcg ctttcgagcc gggcgtgacc gcctgtttcg cgcgcatccg
     8581 gacagtccca ttccggcgcg cgatcgggcg caatggccgg gcgcgtcgtg gtatcgctac
     8641 gacgctgcgt ggcgcacgat cggcgtcgtg aagccgctcg cgcgcacgga cacgttcgac
     8701 atcgcgctcg cggccgacgg cgtcacgcgc tgcacgcgcg ttgcgacggt cgaattcacc
     8761 gtgcgcgcga acgcggcgat gctgacgctg tactggttcg aaggttacgg gggaggtctg
     8821 tggctgccgt tcggcgatgc gagcaacgcc gcgtcgacgt acggcggcgg gcgctatctg
     8881 tacgacacga tcaagggcgc ggatctcggc gcgtcgcaga acgcgttcgt gctcgatttc
     8941 aatttcgcct acaacccgtc gtgcgcgtac gacgagcgct ggtcgtgccc gttgccgccc
     9001 tcggagaatc ggctgccctt cgaggtaacc gccggcgagc gaacgtcgcg atgacggcga
     9061 gccgcgttgc tgccgccgga ttgccggcgt agcatgatgg gataactcga agcgacgttt
     9121 gggaggctgc agtcgccttg gcgaaaggag tcgatcatgt gttacgaatt cgaaagctgg
     9181 cattggaagg cgcgcgccaa ggagatgcac aaggcgcagg tcaggacgga cgcggcggag
     9241 caaaagagcg tgcccgccaa gtcggcgaaa gacactcagc gcgcgcgacc cgaagtgaag
     9301 gagccggaga aggttccggt gtagcgccat tgacccgcgg cgggtgaaat tgctataatc
     9361 gcaatccagt cgcggggtgg agcagtctgg cagctcgtcg ggctcataac ccgaaggtcg
     9421 caggttcaaa tcctgccccc gcaaccaaaa aatcaagggc ttgcagcaat gcaggccctt
     9481 tttgtttggt gcgcccggca gggcgcacgt acttggaggt gaaagtcctc tacacactcg
     9541 gcaaggagaa gtgttagcta aacggcaagg gtgtcgcggg cgactgcgaa tccgg
//
LOCUS       DASSVX010000365         8129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1082591, whole genome shotgun sequence.
ACCESSION   DASSVX010000365 DASSVX010000000
VERSION     DASSVX010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082591"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFO53_15525"
     CDS             complement(<1..703)
                     /locus_tag="VFO53_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET8877738.1"
                     /translation="MPPAPATRRARLRRFLGRHSAVLSLVLGVLIALGGVYGYSVLQP
                     PPHALTQEDIDNAVLHTLDTKQLPSQAARAYEAIRGSVVRVREFERDGPENEITEAGV
                     GTGVVIVDKGIILTNLHVIADAKKVGVIFADGTESEASVIATQPENDLAVLQAKTLPD
                     DLQAATLRSTKDLSLGDEVIAVGFPFGIGPSVSSGVVSGLRREYRSPEGKRLLTNLIQ
                     FDAAANPGNSGGPLVT"
     gene            complement(884..1159)
                     /locus_tag="VFO53_15530"
     CDS             complement(884..1159)
                     /locus_tag="VFO53_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877739.1"
                     /translation="MEPLAVLIVLPVLVGVVAERLFRDTSRASLSAAVTSSLAVYACL
                     RYLDPTGTWNALATFLVAPLAISFSLATVLVCFGYFEGRRTHRRREA"
     gene            complement(1252..1677)
                     /locus_tag="VFO53_15535"
     CDS             complement(1252..1677)
                     /locus_tag="VFO53_15535"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET8877740.1"
                     /translation="MKRSASAMWNGDLKSGNGTISTDSGVLSNTQYSYSTRFENGAGT
                     NPEELIAAAHAGCFSMALSAQLAEAGLVAQSIQTKATVTLEKSGGGFAIPAVHLALTA
                     RIPGASQQAFETAADNAKAGCPVSKLLNATITLDKKLEA"
     gene            complement(1677..1979)
                     /locus_tag="VFO53_15540"
     CDS             complement(1677..1979)
                     /locus_tag="VFO53_15540"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8877741.1"
                     /translation="MKDEQFESALRRDGFAVERRSMPPLAVVAEHAHAFDVRALVLNG
                     EITLTVEGEEYAYREGDIFVLPAGHRHAESVGPAGVDYLVGRRTSDAGSTLERPEA"
     gene            2182..2802
                     /locus_tag="VFO53_15545"
     CDS             2182..2802
                     /locus_tag="VFO53_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877742.1"
                     /translation="MPQRMADAGAGAAAGKSAMTFFITSRASAKGADFGGLAGADQHC
                     QVLAGAAGAGAHTWHAYLSTQGAGAVNARDRIGSGPWRNAKGVVIATSVDELHGNNHL
                     DKQTALTENGDVVNGFGDTPNRHDILTGSRPDGTAFSGTDDMTCGNYTKSGEGSVMVG
                     HADRKGTNPNPVANASWNSSHKSAGCSTELLAKTGSAGYLYCFATN"
     gene            complement(2857..3060)
                     /locus_tag="VFO53_15550"
     CDS             complement(2857..3060)
                     /locus_tag="VFO53_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020682571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HET8877743.1"
                     /translation="MRRPIRGFHQDDERHWVAELECGHFQHTRHDPPWVSRPWVTTAA
                     GREAALGRMLECRKCDAGAPPDR"
     gene            complement(3084..3287)
                     /locus_tag="VFO53_15555"
     CDS             complement(3084..3287)
                     /locus_tag="VFO53_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015486395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transport protein"
                     /protein_id="HET8877744.1"
                     /translation="MNATSLMIGIFTGAIGVGYFMYGKRQTKFVPLIAGMLLCVYPYF
                     VDGVLWLVLVGVALMAAPFLIDF"
     gene            complement(3290..3583)
                     /locus_tag="VFO53_15560"
     CDS             complement(3290..3583)
                     /locus_tag="VFO53_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI-like protein"
                     /protein_id="HET8877745.1"
                     /translation="MHYLLIYELAADYLERRGAFRNDHLKLAWQSDALVLGGALAEPT
                     DRALLLFEGDSPEAATRFANADPYVQNGLVKRWEVRQWTTVVGKFASSPVRPS"
     gene            complement(3568..4101)
                     /locus_tag="VFO53_15565"
     CDS             complement(3568..4101)
                     /locus_tag="VFO53_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET8877746.1"
                     /translation="MQIDATARLRYALTTQWSLAAPIETVWDALALPQAWPQWWSYLE
                     TVVPLAPGDADGIGAVWRYTWSSRLAYRLTFDMTTTASRYPELIEGTAAGELRGRGCW
                     RLARDGGNTRVRYTWIVATGKPWMSVLAPLLRRVFEWNHDQVMRAGGRGLARHLGVRL
                     LAYDRLKNDEDGTCTTC"
     gene            complement(4101..4556)
                     /locus_tag="VFO53_15570"
     CDS             complement(4101..4556)
                     /locus_tag="VFO53_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877747.1"
                     /translation="MASHVYAWNPKLWNWPEQPRERRAIARRGHADIEWACGRNRAIE
                     VGSRAFFVRLGVPPKGLFGSGYALCEPWEDVHWLDERAQLGARTRYVKLRLDTLFDAP
                     LISLDELARPPFGRFRWAVRQSGVRLPSSLADLLEPWWEARVADARRNA"
     gene            4635..5564
                     /locus_tag="VFO53_15575"
     CDS             4635..5564
                     /locus_tag="VFO53_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET8877748.1"
                     /translation="MSRSYFAAAVVLLAAACASVPPRTIVPRAAPPVGGKYYLDDGPG
                     SNPPPDLDAIPDAVPRVEALSRAANRPYAVFGREYVPATALKPYRERGVASWYGRKFH
                     GQKTSIGEPYDMYAMTAAHPTLPLPSYARVTSVATGRSVVVRVNDRGPFLHDRIIDLS
                     YAAAHRLGIAQNGSGEVDVEAILPGTLAASAQAAQPPLAAVADAPVITAPLAPPAQVQ
                     PATLDADAAPVGGMRGGYSVQLGAFASFSNAQAFLARAQNQLATVQVEAKIRQAGGLY
                     RVYIGPYPARSDAERLGERITQAFGFPTTVAAH"
     gene            5693..6985
                     /gene="tolB"
                     /locus_tag="VFO53_15580"
     CDS             5693..6985
                     /gene="tolB"
                     /locus_tag="VFO53_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HET8877749.1"
                     /translation="MIPLLRRWLAFVALTFLPFGTQLAHAQLSIEIVGGAGTAIPIAV
                     VPFDAEGTWPLAVSGIVGADLARSGLFRLVDTAGVVPRPVRAEDVQSATWRARNADAV
                     VVGSMLPLPDGRADVRFALVDVVKQTALVQMSFTVAPAQFRVTAHKIADIIYEKLTGD
                     RGVFSTRIAYVTKQGPRFQLLVADADGYGPQSIVSSNEPLLSPRWSPDGTRLAYVSFE
                     NKKPVVYVQSLDTGARRAVANFRGSNSSPAWSPDGRKLAVTLTKDGGSQVYLMNPDGS
                     GVTRVLTSQAIDTEAYFTPDGQSLVFTSDRGGTPQIYRANLLGGQVERLTFEGSYNVS
                     PKPLPDGKGIVFVRRDGGRFQIATLDYATRQVQVLTGGPNDESPSIAPNGKLVIYASE
                     GGSRGILAAVSIDGRVKQRLVAPATDVREPAWGPFPRS"
     gene            7048..7599
                     /gene="pal"
                     /locus_tag="VFO53_15585"
     CDS             7048..7599
                     /gene="pal"
                     /locus_tag="VFO53_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HET8877750.1"
                     /translation="MRNLLFVAIVAAALAGCSTTPTTEAPVEDHGTAGTTAPGTGAET
                     GGARGGGVNGSALGAGAGGDELRNPNSILSKRSVYFDFDSFVVKDEYRPLVEAHARYL
                     QSHRNARMTVQGNTDERGSREYNIALGQKRADAVKRMMTLLGAQDAQIETTSFGEERP
                     KNPGHDESAWAENRRDDIVYGGE"
     gene            7668..>8129
                     /locus_tag="VFO53_15590"
     CDS             7668..>8129
                     /locus_tag="VFO53_15590"
                     /inference="COORDINATES: protein motif:HMM:NF027655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgF trimerization domain-containing protein"
                     /protein_id="HET8877751.1"
                     /translation="MRSVIRATLAGVALALVAHAAQAALFDDDEARRRVEATNIRLSQ
                     LERRVEERLAALEQQVKGQGLADIANQLQLLQADVAKLRGQVEVVTYELEQSQKRQRD
                     LYVDLDTRMRKLESAAATERSAPATPGAAAEGGAAGTPPAEAAAPPPAAATA"
BASE COUNT         1343 a         2795 c         2738 g         1253 t
ORIGIN      
        1 tcgtcacgag cggcccgccg gaattgcccg gattggccgc cgcgtcgaac tggatcaggt
       61 tggtgagcaa gcgcttacct tccggcgaac ggtattcgcg acgcaatccg gaaaccacgc
      121 ccgagctgac cgacgggccg atgccgaacg ggaacccgac cgcgatcact tcgtcgccga
      181 gggacagatc cttcgtcgag cgcagcgtgg ccgcctgcag gtcgtcgggc agcgtcttcg
      241 cctgcaggac agcgaggtcg ttttcgggct gcgtcgcgat cacgctcgct tccgattcgg
      301 ttccgtcggc gaagatgacg ccaaccttct tcgcatcggc gatgacgtgc agattggtga
      361 ggatgatgcc cttgtcgacg atgacgacgc ccgtcccgac accggcttcg gtgatctcgt
      421 tctcgggtcc gtcgcgctcg aattcgcgca cgcgcaccac cgagccgcgg atcgcctcgt
      481 aggcgcgcgc cgcctgcgac ggcagctgct tcgtgtcgag cgtgtgcagg accgcgttgt
      541 cgatgtcctc ctgcgtgagc gcgtgcggcg gcggttgcag cacgctgtaa ccgtagacgc
      601 caccgagcgc gatcagcacg ccgagaacga gcgacagcac ggcgctgtgg cggccgagga
      661 agcggcgcag gcgcgcgcgg cgcgtggcgg gcgccggcgg cacatcggtc ggtgaattcg
      721 ttgcggcggg cgcgcccgca ctggccggcg atgccgcgtc tccgccgggc ggaagcgccg
      781 tccggggacg ctgcgagctg ctgtagaggg atgcgcgccg catggaaaag acagtaaggg
      841 ctctcgctga agacaacttc ccgcgccgag gattcaatcc ccgctaggcc tcgcgccggc
      901 gatgcgtgcg gcgtccctcg aagtagccga agcagacgag caccgtcgca agcgaaaacg
      961 agatcgcgag cggcgcgacg aggaacgtcg ccagtgcatt ccaggtgccg gtgggatcga
     1021 ggtagcgcag gcacgcatac accgccagcg acgaggtgac ggccgccgaa agcgacgccc
     1081 gcgacgtgtc gcggaacagc cgctccgcga cgacgccgac cagcaccggc agcacgatca
     1141 ggaccgcaag cggctccatg gcggcactcg catcatcggc acgaaacgtt ccggactaat
     1201 ggtactccgc cgtacggcat tgtccatgca cgctcacggg cattgaaccg ttcacgcctc
     1261 gagcttcttg tcgagggtga tcgtcgcgtt cagcagcttc gataccgggc aacccgcctt
     1321 cgcgttgtcg gcggccgttt cgaacgcctg ctggctggcg cccgggatgc gtgcagtgag
     1381 cgcgagatga acggcgggaa ttgcgaagcc gccacccgac ttctcgagcg tgaccgttgc
     1441 cttcgtctga atgctttgcg cgacgaggcc cgcttcggcg agttgcgccg acagcgccat
     1501 cgagaagcag ccggcgtgcg ccgcggcgat gagctcctcg ggattggtgc ccgcgccatt
     1561 ctcgaaccgc gtgctgtagg aatactgcgt gttcgacagg acgccgctgt cggtggagat
     1621 cgtgccgttg cccgatttta ggtcgccgtt ccacattgcc gatgcgctgc gtttcatcat
     1681 gcctccggtc gttcgagggt tgagcccgcg tcggaagtgc gccggccgac gaggtagtcc
     1741 acgccggccg gcccgaccga ttccgcgtgg cggtgcccgg ccggcagcac gaagatgtcg
     1801 ccttcgcggt acgcgtattc ctcgccttcg accgtcaacg tgatctcgcc gttcagcacc
     1861 agcgcgcgga cgtcgaacgc atgcgcgtgc tcggcgacca cggcgagcgg cggcatcgac
     1921 cggcgttcca cggcgaagcc gtcacggcgc agcgccgact cgaattgctc gtccttcatg
     1981 tcgtcctcca tgccttacgt ccacgacgaa ggtgcgcgcc gcggaatagc ggcgcggcgc
     2041 ttgatgttga cagtgccccg cgggcttggc aacctgcccg tcgaccgacc tgcccgtcaa
     2101 ccgacaggag tgcccgtgac agcttcgaaa tccggaatcg cgttcgcaac tgcattgctc
     2161 gtcgcgggct gcagcgacat gatgccgcaa cgcatggccg atgcgggagc gggtgccgcg
     2221 gcgggcaagt ccgccatgac gtttttcatc accagtcgcg catcggcgaa gggtgccgac
     2281 tttggcgggc tcgcgggcgc cgatcagcac tgccaggtgc tggccggtgc ggcgggcgcc
     2341 ggcgcgcaca catggcatgc ctatctcagc acgcagggcg cgggcgcggt caacgcgcgc
     2401 gatcgcatcg gcagcgggcc ctggcgcaat gccaagggcg ttgtcatcgc taccagcgtc
     2461 gacgagttgc atgggaacaa tcatctcgac aagcagactg cgcttaccga gaacggtgac
     2521 gtcgtcaacg ggttcggcga cacgccgaac cggcacgaca tcctcaccgg atcgagaccc
     2581 gacggcacgg cattcagcgg caccgacgac atgacctgcg gcaattacac gaaaagcggc
     2641 gaaggcagcg tcatggtggg ccacgccgac cggaaaggca cgaacccgaa cccggtcgcc
     2701 aatgcgtcgt ggaattcgtc gcacaagtcg gccggctgca gcacggagct cctggcgaag
     2761 accggcagcg ccggatacct gtattgcttc gcgacgaact gaaaaccgaa atagggtcag
     2821 agacgacttt cgcgttcggg aaagtcgtct ccgaccctac ctgtcgggtg gcgcgcccgc
     2881 atcgcacttc ctgcattcga gcatccggcc aagcgccgct tcccggccag cggcagtcgt
     2941 tacccacggg cggctgaccc acggcgggtc gtggcgcgtg tgctggaaat ggccgcactc
     3001 gagctccgcg acccagtggc gttcgtcgtc ctgatgaaaa ccgcggatcg gccggcgcac
     3061 cgttgcatcc gggccgtcgg ccgctagaaa tcgatcagga atggcgccgc catcagcgcc
     3121 acgcccacga gcaccagcca cagcacgccg tcgacgaaat acggatagac gcagagcagc
     3181 atcccggcga tgagcggcac gaacttcgtc tgccgcttgc cgtacatgaa atagccgacg
     3241 ccgatcgcac ccgtgaagat gccgatcatg aggctcgtcg cgttcatcgc taagacgggc
     3301 gcaccggtga cgacgcgaac ttgccgacga cggtcgtcca ttgccgcact tcccagcgct
     3361 tgacgaggcc attctgcaca tacggatcgg cgttcgcgaa gcgcgttgcg gcctcgggcg
     3421 aatcgccttc gaacaacaac agcgcgcggt cggtgggctc ggcgagcgcg ccgccgagca
     3481 cgagcgcatc ggactgccag gcgagcttca ggtggtcgtt gcgaaaggcg ccgcggcgct
     3541 ccagataatc ggcggcgagc tcgtatatca gcaggtagtg catgtgccgt cctcgtcgtt
     3601 tttcaggcgg tcgtacgcga gcaatcttac gccgagatgc cgcgcgaggc cgcggccgcc
     3661 ggcacgcatc acctggtcgt ggttccattc gaagacgcgc cgaagcagcg gcgcaagaac
     3721 gctcatccag ggcttgccgg tggcgacgat ccacgtgtag cgaacgcgcg tgttgccgcc
     3781 gtcgcgcgcc agtcgccagc atccgcgacc gcgaagctcg cccgccgcag tgccttcgat
     3841 cagttccgga taccgcgacg ccgtcgtcgt catgtcgaag gtgaggcgat acgcaagccg
     3901 gctcgaccac gtgtagcgcc acacggcgcc gataccgtcc gcatcgccgg gcgcgagcgg
     3961 cacgaccgtc tcgaggtacg accaccattg cggccacgcc tgcggcagcg cgagcgcatc
     4021 ccagacggtc tcgatcggcg cggcgagcga ccactgcgtc gttagcgcat agcggagtcg
     4081 cgcggtggcg tcgatctgca tcaggcattg cgtcgcgcgt ccgccacgcg cgcctcccac
     4141 cacggctcga ggaggtcggc gagcgacgac ggcagtcgca cgcccgactg gcgcacggcc
     4201 cagcggaacc ggccgaacgg cggacgcgcg agctcgtcga gcgagatcag cggggcgtcg
     4261 aacagcgtgt cgagccgcag tttcacgtag cgcgtgcgcg cgccgagctg cgccctttcg
     4321 tcgagccaat ggacgtcctc ccacggctcg cacagcgcat agccggaccc gaacaggccc
     4381 ttcggtggca cgccgagacg cacgaagaac gcgcgcgatc ccacctcgat cgcgcggttg
     4441 cgtccgcatg cccactcgat gtcggcgtgg ccgcggcgtg cgatggcacg ccgctcgcgc
     4501 ggctgctcag gccagttcca cagcttcgga ttccacgcgt acacatggct tgccatcggt
     4561 atcgctcttt ggaaattgtt ccgcgcgagc tcgcgcgatg gtagattccg cgcgggaaaa
     4621 ccgctcgcgc cgccatgtcc cgctcgtatt ttgccgctgc cgtcgtgctg ctcgccgctg
     4681 cctgcgcgag tgttccaccg cgcaccatcg tcccgcgggc cgcgccccct gtcggcggca
     4741 aatattatct ggacgacggc ccgggatcga atccgccgcc cgatctcgac gccattcccg
     4801 acgccgtgcc gcgcgtcgag gctttgagcc gcgccgcgaa ccgtccctac gcggtgttcg
     4861 ggcgcgaata cgtgcccgcg acggcgctga agccgtatcg cgagcgcggc gtcgcgtcgt
     4921 ggtacgggcg caagttccac ggacagaaga cgtcgatcgg cgagccgtac gacatgtacg
     4981 cgatgaccgc cgcgcatccg acgctgccgc tgccatcgta cgcgcgcgtg acgagcgtcg
     5041 cgacgggcag gagcgtggtt gtgcgcgtca acgatcgcgg gccgttcctg cacgatcgca
     5101 tcatcgacct gtcgtacgcc gccgcacacc ggctcggcat cgcgcagaac ggcagcggcg
     5161 aggtggacgt cgaagcgatc ctgccgggta ccctcgccgc gtcggcacag gccgcacagc
     5221 cgccgcttgc agcggtcgcc gacgccccgg tgattaccgc gccgctcgca ccgcccgcac
     5281 aagtgcaacc ggcgacactg gatgccgatg cagcgccggt cggcgggatg cgcggcggct
     5341 attcggtcca gctcggggcc ttcgccagct tttccaacgc gcaggcattc ctcgctcgcg
     5401 cgcagaacca actcgcgacc gtgcaggtcg aagcgaaaat tcgacaagcg ggcggactct
     5461 atcgcgtcta tatcggccct taccctgcgc gcagcgacgc cgagcgcctc ggggagcgca
     5521 tcacgcaggc cttcggtttc ccgaccacgg ttgccgcgca ttgataacgc ggggatgccc
     5581 gccccttcgt tataatgagc accgcgcgcc aaggtgagcg cgcgtcgccc gcgcgaacgc
     5641 gccgtttgcg ttcccgcggt tccgttggct cctcccgctg caccttcgct cgatgatccc
     5701 gttgcttcgt cgctggctcg cctttgttgc gctgacgttt ttgccgttcg gcacgcaact
     5761 cgcacacgct caactgtcga tcgaaatcgt cggcggggcg ggcaccgcca ttccgatcgc
     5821 cgtcgtcccg ttcgatgccg aaggcacgtg gccgctggcc gtatcgggca tcgtcggcgc
     5881 cgatctcgcg cgctcgggac tgttccgtct cgtcgacacc gccggcgtcg tgccgcgacc
     5941 ggtgcgtgcc gaggacgtgc aatcggccac gtggcgcgcg cgcaacgccg atgcggtggt
     6001 cgtcggctcg atgctgccgc tgcccgacgg gcgcgccgac gtgcgcttcg cgctcgtcga
     6061 cgtcgtcaag cagacggcgc tcgtgcagat gtcgttcacc gtggcgcccg cgcagttccg
     6121 cgtcaccgcg cacaagatcg ccgacatcat ctacgagaag ctgaccggcg accgcggcgt
     6181 gttctcgacg cgcatcgcct acgtgacgaa gcaggggccg cgcttccagc tgctggtggc
     6241 cgatgccgat ggctacggtc cgcagtcgat cgtctcgtcg aacgagccgc tgctgtcgcc
     6301 gcgctggtcg cccgacggca cgcggctcgc gtacgtctcg ttcgagaaca agaagccggt
     6361 cgtgtacgtg caatcgctcg acaccggcgc gcggcgcgcg gtcgccaact tccgcggcag
     6421 caattcgtcg ccggcgtggt cgcccgacgg gcgcaagctg gccgtcacgc tgaccaagga
     6481 cggcggctcg caggtgtacc tgatgaatcc cgacggcagc ggcgtgacgc gcgtgctcac
     6541 gtcgcaggcg atcgacaccg aggcctactt cacgcccgac ggccagtcgc tcgtgttcac
     6601 gtccgatcgc ggcggcacgc cgcaaatcta tcgcgcgaac ctgctcggcg ggcaggtcga
     6661 gcgcctcacg ttcgagggca gctacaacgt ttcgccgaag ccgctccccg acggcaaggg
     6721 catcgtgttc gtgcgtcgcg atggcgggcg cttccagatc gcgaccctcg attacgccac
     6781 gcgtcaggtg caggtgctga ccggcggccc gaacgacgaa agtccgagca ttgcgccgaa
     6841 cggcaagctc gtcatctatg cgagcgaagg tgggagccgg ggcatcctgg ccgcggtgtc
     6901 catcgacggt cgcgtgaaac agcggctcgt ggcacccgcg accgacgtgc gcgagcccgc
     6961 ctggggaccg tttccgcggt catgaaattt catcctttcg atcgcgcgcc gggtcgcggt
     7021 gcgcacgaac ttactgcagg aggtggcatg cgcaatttac tttttgtcgc gatcgttgcg
     7081 gcggcactgg ccggttgctc gacgacgccg acgaccgaag cacccgtcga ggaccatgga
     7141 acggccggca cgacggcgcc cggcaccggt gccgaaaccg gcggcgcgcg cggcggcggc
     7201 gtgaacggtt ctgcgctcgg cgccggcgcg ggtggcgacg agttgcgcaa tccgaacagc
     7261 atcctgtcga agcgcagcgt gtacttcgac ttcgacagct tcgtcgtcaa ggacgagtac
     7321 cggccgctcg tcgaggcgca cgcgcgctat ctgcagtcgc atcgcaacgc gcggatgacg
     7381 gtgcagggca acaccgacga acgcggctcg cgcgagtaca acatcgccct cggacagaag
     7441 cgcgccgacg cggtcaagcg catgatgacg ctgctcgggg cgcaggacgc ccagatcgag
     7501 acgaccagct tcggcgagga gcgcccgaag aaccccggtc acgatgaatc ggcgtgggcg
     7561 gaaaatcgcc gcgacgacat cgtgtatgga ggcgagtgat cgaccgctcg tctcgcgcga
     7621 tgcacgggaa gacgtgaccg gtcgatgaac gcgaggattt gccgccaatg aggtcagtca
     7681 tccgagcaac gctcgccggc gttgcactcg ccctcgtagc gcacgccgcg caggcggcgc
     7741 tgttcgacga cgacgaggcg cgccggcgcg tcgaggccac gaacatccgg ctgtcgcagc
     7801 tcgagcgccg cgtcgaggag cgcctggcgg cactcgagca gcaggtgaag ggccagggtc
     7861 tcgccgatat cgccaatcag ctgcagcttc tgcaggccga cgtcgcgaag ctgcgcggac
     7921 aggtcgaggt cgtcacctac gagctcgagc agtcgcagaa gcgccagcgc gacctctacg
     7981 tcgatctcga cacgcggatg cgcaagctcg aatcggcggc ggcaaccgag cgaagcgcgc
     8041 cggccacgcc gggcgccgcc gccgagggcg gcgcagcggg cacgccgcca gcggaagcgg
     8101 ccgcaccgcc gcccgccgcg gcgactgcg
//
LOCUS       DASSVX010000366        11474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1366086, whole genome shotgun sequence.
ACCESSION   DASSVX010000366 DASSVX010000000
VERSION     DASSVX010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 11474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11474
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366086"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFO53_15595"
     CDS             <1..276
                     /locus_tag="VFO53_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8877752.1"
                     /translation="GRERRAVVKLSGYFKFSRTPHPHDDVRAHMEALLDAFTPDACVW
                     ASDWPFLRAPRHIDVGPLLTLLERLVPDAADRRKVLWETPRRVLGFG"
     gene            308..1708
                     /gene="asnS"
                     /locus_tag="VFO53_15600"
     CDS             308..1708
                     /gene="asnS"
                     /locus_tag="VFO53_15600"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET8877753.1"
                     /translation="MKRFSCSEILSGRAPPDVPVVLAGWVRTRRDSKAGISFVHVSDG
                     SCFDPVQAVVPNTVPNYADEVVHLTAGCAVEITGTVVASPARGQPFEMQASAVRVVGW
                     VDDPDSYPIQPKPHTLEFLRDVAHLRPRTNVIGAVTRVRDSIAHSIHRFFHDHGFFWI
                     NTPIITSSDAEGAGELFRVSTLDLANLPRTAQGAVDHAQDFFGRETFLTVSGQLNVEA
                     YCLALSKVYTFGPTFRAENSNTSRHLAEFWMIEPEIAFADLAADATLAEALLKHVFRT
                     VLEERADDMAFFEQRIEKGVIAKLTGIVDSEFIRMDYTEAIRVLSEATQTFQFPVSWG
                     MDLQSEHERYLSETYAKKPVIVMNYPKAIKAFYMRVNDDGNTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDARMDEAALDKAHYAWYRDLRRYGSVPHAGFGLGFERTIAYATGLSN
                     VRDVIPFPRTPGSARY"
     gene            complement(1724..3256)
                     /locus_tag="VFO53_15605"
     CDS             complement(1724..3256)
                     /locus_tag="VFO53_15605"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HET8877754.1"
                     /translation="MIRAARHRRRGYRAGVVSLALAWVAAAGAAEPGAGATDLPAAWA
                     AARSADTERAELTVPGVAPDSMLQDERARRSRSDAAEIDARKSYAIPAAEILGFEFLL
                     NQFDRHVLGTDFASNASTIRRNLHSSWVVDRDPFQINQLGHPYAGSMYHAFARSAGLG
                     YWESLGYTFAGSALWEIAGERSLPSRNDQINTGIGGSFLGEILFRLSNLTLEHENLPP
                     FWRETAAALISPPVGFNRLAFGDRFRTIFPSNDPIYFSRLQVGFSRSVQNSAGASAAD
                     FKRSEALIDYSIDYGLPGRPGYTYARPFDYFSLEATASSANGAESLLTRGLLVGKDYE
                     AGHDYRGVWGLYGLYDYIAPQTFRVSSTAAALGTTAQWKLSETMALQGTALAGAGYTA
                     VGTTRSTSDNDYHYGVSPQALLALRLILGDRVALDLTGREYFVSRVGAAARGGHDNIV
                     RLDASLTFRVHDRHGISIRYLLNRRDAFYPDVGDSSQTRGTIGLFYTYLGHQRFGYVP
                     WE"
     gene            complement(3279..3689)
                     /locus_tag="VFO53_15610"
     CDS             complement(3279..3689)
                     /locus_tag="VFO53_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HET8877755.1"
                     /translation="MNSLLIKVATAGAAAIMTMAGAACATTSDQAAPTSSSAAATTLA
                     QSTPQSATPATASTPSPATPQSSAMPPTSSTPPPSPSTTGSAQSDASAKPAPSTSATP
                     SQEVEARKIFDQLDANHDGTLSFDEFSRATFRAK"
     gene            3992..4441
                     /locus_tag="VFO53_15615"
     CDS             3992..4441
                     /locus_tag="VFO53_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877756.1"
                     /translation="MRKALWSCITACSLLAGCAAMHPQPQRVLNHASCHAGSACPVDV
                     KVTCDHFYGCDISVDPDLIVVEDRGQRTDIVWRLSGDTGARFPANGVVFDDSLLKCEP
                     RADGREFVCGDTPPGFGVFKYRVSVTVAQSLFGPRGALSLDPWVVNR"
     gene            complement(4472..7120)
                     /locus_tag="VFO53_15620"
     CDS             complement(4472..7120)
                     /locus_tag="VFO53_15620"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8877757.1"
                     /translation="MQEQPAVTTFLFTDIEGSTRLWDQEPERMREALARHDALARSAI
                     ETRQGTLVKTTGDGVHAVFGDPLDGVMAVVDLARRLDDPQATGGLTLRVRCGLHAGVV
                     EHRDNDYFGTAVNRAARIMSAAHGGQILVSQSVAELLGGRLPGDVTLLDLGVVRLRDL
                     ATPERLFQLVHSALRATFPPLRSLEAIPNNLPQQLTSFIGRERELGEIGALLRTSRLV
                     TITGTGGLGKTRLSLQVAADVLDEFQDGAWLVDLASLADARLVAQAAAAALAVKEDAG
                     RSITDALVRHCQDRAILLVLDNCEHLIDACAEFAVQLLQSTKDVRILATSRERLNVRG
                     ERAYPLAPMLVPDRDAPHAATTLAQSDPVRLFIDRIAASQPGLEANAQTITDIAEICR
                     RLDGIPLAIELAAARARVLPIATIAARLDNRFRLLTGGDRTALPRQQTLRALIDWSYE
                     LLSEPERTLFRRLAVFAGGFTMEDAESIASDAALPDAAVMDVLAKLVDKSLVVLDADG
                     ARYRVLETVREYARERLEGSGEANVLRDSHLRHYVAVAESAVPELFGPRQGEVFARLD
                     GERENLLAAHARCASAQDGVDLGLRLATLKFYWIHRGEPGLGYRLGVEALARTRGDDR
                     SVVRCRALFDVGQMACYMGRYGEGRRHLEESLAIARELGNDGRIASALQPLAMACLGL
                     GEAAVAQHHLEEALVLVRAQGNKHDLASALNAMAQVHRARNDLDAALPLYEQVVGLAR
                     ETGDREVVAFGLLNLAMALIARNGSDRVPAILLEALAIADENRSTPATQSVLEVSAGW
                     AAAKGDWKRAARLFGVAEALAETTGLRRDPADEAFLAPLIDRARAALGAPAFGSIEAA
                     GRVVSRDAAIAEVRGWIAASAQPAPA"
     gene            7203..7279
                     /locus_tag="VFO53_15625"
     tRNA            7203..7279
                     /locus_tag="VFO53_15625"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7237..7239,aa:Arg,seq:tct)
     gene            7742..9883
                     /locus_tag="VFO53_15630"
     CDS             7742..9883
                     /locus_tag="VFO53_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HET8877758.1"
                     /translation="MAEQKSKMPFRRAREFEKRKLLQVSIASALAAGMVIAASADART
                     TSIQITSQGIAFGGYAFPGIGQYEKITGIATGEVDPNDPRNAVITDIALAPRNANGNV
                     VYQHNFYILKPVDPSKGNHQVMYEPPNRGGKTYASLNRTPVGGNLTNDPGAITDATLL
                     AGSFLWSRGYTTVWSGWENNLGPLGGLTATAVLPVATNGGETITGPAYDYIVNPPGGT
                     YALTYPAASGTKSPVALLTHRVHLNDPPVAVPDSGWDYTDATRTAIRLTGSSFITNDI
                     YEFSYTAMNPTVNGLGFAAVRDFNSFIRYDTTAANPLAGDVTRIYTEISSQPGRLLND
                     FTNLGFNEDESGRKVIDGMMQWIAAGDGINMNYRWSQTKRTNRNRQELLFLEGKFPFA
                     NVETTDPHTGKTDSRFARCEKSNTCPVAAEIYSANEYWVKGASLLHTDPAGTVDLPDS
                     PYARDYFISSHQHGTGNAASRGACQQFQNPLNSAPIQRALFLALDDWVNGTAPPPSRV
                     PKLADGTLVPPLPQSAVGFPHIPGVTYTGLETTRYLFDFGPNFYETGVPTINPPHVSA
                     PYQDNPANGPIYPTFVPKTDSDGNDIAGVRLPDVTVPLATYTGWALRAGVWANDGCEG
                     SGQYIPFPRTKVDRVASGDPRPSVEERYKSFGQYRSAVVNAVDDLVKKRLMLCEDAGD
                     QVHRLVDAGLAAGVPAPTGPEPMYPDVPHCN"
     gene            10066..11067
                     /gene="dctP"
                     /locus_tag="VFO53_15635"
     CDS             10066..11067
                     /gene="dctP"
                     /locus_tag="VFO53_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019019003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET8877759.1"
                     /translation="MKSITPVGMLAALALALASGSLAAAPVVLRASHQFPGGKGDVRD
                     EMVQIIARDAKAAGVDLDVQVYPGAALFKPNEQWNALVNGQLDISSFPLDYASGKVRA
                     FGATLMPGLVRNHERAARLNDSPFMKEIKAKIEKAGVVVLSDSWLAGAVASKKGCIRT
                     PADMQGLKIRSAGPTFAEMWQAAGASIVSVPSNEVYNALQTGVADATDTSTSSFVSFR
                     IYEQVKCITAPGENALWFMYEPVLMSKKSFDRLDKKQQAVLLAAGKKAEAFYAKEGKK
                     LDDEMVKAFKDHNVEVVTMTPAEYDQWIAIAKKSSYADFAKEVPDGKKLIDDALSVK"
     gene            11123..>11474
                     /locus_tag="VFO53_15640"
     CDS             11123..>11474
                     /locus_tag="VFO53_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HET8877760.1"
                     /translation="MKYLPSDGMLDRFARVVRALSQLCGFIAAGLVALAVVIVCEMVF
                     VRYVLNQSTIWQTDFITYCITAATFIGSPYVLMTRGHVNVNVLPLHLAPRGRFALALF
                     AALTSAAFCVVMAVM"
BASE COUNT         1908 a         3813 c         3845 g         1908 t
ORIGIN      
        1 gggcgcgaac ggcgcgcggt cgtcaagctc tcgggctatt tcaagttttc gcgcacgccc
       61 catcctcacg acgacgtccg cgcgcacatg gaagcgcttc tcgacgcgtt cacgcccgac
      121 gcctgcgtct gggcgtccga ctggccgttt ctgcgcgcgc cccggcacat cgacgtcggg
      181 ccgctgctga cgctgctcga gcgcctcgtg cccgatgccg ccgatcggcg caaggtgtta
      241 tgggagacgc cgcggcgggt gctgggcttc gggtagagtg tccgcttttg cggcgttcgc
      301 cattgcgatg aagcgctttt cctgcagcga aattctgtcc ggacgggcgc cgcccgacgt
      361 cccggtcgtg ctcgcgggct gggtgcgcac gcgacgcgac tcgaaggccg gcatctcgtt
      421 cgtgcacgtt tccgatggct cgtgcttcga cccggtgcag gccgtcgttc cgaataccgt
      481 cccgaattac gccgacgagg tcgtccatct gacggcgggg tgtgcggtcg agatcaccgg
      541 caccgtggtg gcgtcgccgg ccaggggcca gccgttcgag atgcaggcga gcgccgttcg
      601 tgtcgtcggc tgggtcgacg atcccgattc ctatccgatc cagccgaagc cgcacacgct
      661 cgaattcctg cgcgacgtcg cgcacttgcg tccgcgcacg aacgtcatcg gcgcggtgac
      721 gcgcgtgcgc gactcgatcg cgcattcgat ccaccgcttc ttccacgacc acggcttctt
      781 ctggatcaac acgccgatca tcacgtcgtc ggatgccgag ggcgccggcg agctcttccg
      841 cgtgtcgacg ctcgacctcg cgaacctgcc gcgcaccgcg caaggcgcag tcgaccacgc
      901 gcaggatttc ttcgggcgcg aaacgttcct gacggtgtcg gggcagctca atgtcgaggc
      961 gtactgcctc gcattgtcga aggtgtacac attcggtccg acctttcgcg cggagaattc
     1021 gaacacgagc cgtcatctcg ccgagttctg gatgatcgag cccgagatcg cattcgccga
     1081 tctggccgcc gacgcgacgc tcgccgaagc gcttctgaag cacgtcttcc gcacggtgct
     1141 cgaggaacgg gcggatgaca tggcgttctt cgagcagcgg atcgagaagg gcgtgatcgc
     1201 gaagctcacc ggcatcgtcg attccgaatt catccgtatg gattacaccg aggcgatccg
     1261 cgtgctgagc gaggcgacgc agacgttcca gttcccggtg agctggggca tggatctgca
     1321 atccgagcac gagcgctacc tgtccgagac ctacgcgaag aagccggtga tcgtgatgaa
     1381 ctacccgaag gcgatcaagg cgttctacat gcgcgtcaac gacgacggca acacggtggc
     1441 ggcgatggac gtgctggcgc ccggcatcgg cgagatcatc ggcggcagcc agcgcgagga
     1501 gcggctcgac gtgctcgatg cgcggatgga cgaggctgcg ctcgacaagg cgcattacgc
     1561 ctggtaccgc gatctgcgcc gctacggcag cgtgccgcac gcggggttcg gcctcggctt
     1621 cgagcgcacg atcgcgtatg caaccggtct ttccaacgtg cgcgacgtga ttcccttccc
     1681 gcgtacaccg ggcagcgcgc gctactgatt cacgcgtgcg cgcctactcc cacggcacgt
     1741 agccgaagcg ctggtgcccg aggtaggtgt agaaaagccc gatcgtgccg cgcgtctgcg
     1801 agctgtcgcc gacgtcggga tagaacgcgt cgcgccggtt cagcagatac cggatcgaga
     1861 tgccgtggcg gtcgtggacg cggaacgtga gggacgcgtc gagacgcacg atgttgtcgt
     1921 gcccgccgcg cgcggccgcg ccgacgcggc tcacgaaata ttcgcggccg gtgaggtcga
     1981 gtgcgacgcg gtcgccgagg atcagccgca acgccagcag cgcctgcggc gataccccgt
     2041 agtggtagtc gttgtcggac gtgctgcgcg tcgtgccgac cgcggtgtag ccggcaccgg
     2101 ccagcgcagt tccctgcaac gccatcgtct ccgagagctt ccactgcgcc gtggtgccga
     2161 gcgcggccgc cgtgctcgac acgcgaaagg tctgcggcgc gatgtagtcg tacaggccgt
     2221 acagtcccca cacaccgcgg tagtcgtggc ccgcttcgta gtccttgccc acgaggaggc
     2281 cgcgggtcag caggctttcg gcgccgttcg cgctcgaggc cgtggcttcg aggctgaagt
     2341 agtcgaaagg ccgcgcatag gtgtaaccgg gacggcccgg caagccatag tcgatcgagt
     2401 agtcgatcaa cgcttcgctg cgcttgaagt cggccgccga ggcgccggcg ctgttctgca
     2461 cgctgcggct gaagccaacc tgcagcctgc tgaaatagat cgggtcgttg ctcgggaaga
     2521 tcgtgcggaa acgatcgccg aacgcgagcc ggttgaagcc caccgggggc gagatcagcg
     2581 ccgccgcggt ttcgcgccag aacggcggca ggttttcgtg ctcgagcgtg agattcgaca
     2641 aacggaacag gatctcgccg agaaagctgc ccccgatgcc ggtgttgatc tggtcgttgc
     2701 gcgagggcag gctgcgctcg ccggcgatct cccacagcgc gctgccggcg aacgtgtagc
     2761 ccagggattc ccagtaaccg agccccgcgg aacgcgcgaa cgcgtggtac atcgaccctg
     2821 cgtacggatg accgagctga ttgatctgga acggatcgcg gtcgaccacc cagctgctgt
     2881 gcaggttgcg ccgtatcgtc gatgcgttgc tcgcgaagtc ggtgccgagc acgtgccggt
     2941 cgaactggtt cagcaggaat tcgaatccga gaatctccgc ggccgggatc gcatagcttt
     3001 tgcgcgcgtc gatttccgca gcgtcgcttc gcgagcgtct tgcacgctcg tcttgcaaca
     3061 ttgaatcggg cgcgacgccg ggcacggtga gctcggcgcg ctcggtgtcg gcgctgcgcg
     3121 cggcggccca ggccgccggc agatcggttg cgccagcacc tggctcggcg gctccggccg
     3181 cggccaccca cgcgagtgca agcgagacga cgcccgcacg gtagccgcgt cgccggtgtc
     3241 gcgccgcgcg aatcatcggg gatgcccgac gcgtgcgctc attttgcgcg gaaggtcgca
     3301 cgcgagaatt cgtcgaacga cagcgtgccg tcgtggttcg catcgagctg gtcgaagatc
     3361 ttgcgtgcct cgacctcctg cgacggggtg gcgctcgtcg atggtgccgg cttcgcggac
     3421 gcatcgcttt gcgcgctgcc ggtcgtcgac ggcgaaggcg gcggtgtcga tgacgtcggc
     3481 ggcatggccg acgattgcgg cgtggccggc gacggcgtcg acgccgtggc gggcgtggcc
     3541 gactgcggcg tcgactgtgc gagcgtggtg gctgcagcac tcgacgacgt cggcgccgcc
     3601 tggtcggatg tcgttgcgca cgcggcgccg gccatcgtca tgatcgccgc cgcgcccgcg
     3661 gtggcgactt ttatcagcag ggaattcatc gcgactctcc tttcattcga tgggggcggc
     3721 ggacgccgcc gatgacgcct ggcgatgcaa gtagcctgcc ggaatgcaac gcaatgattt
     3781 acatggacgc ggggcgttcg cggtagccct gcgaaccggc gccgcgtgta gcgcataccg
     3841 tccgtgtagg agaaacaccg gcagccgctt gccgcgcgcg ccgggccgca tcggcaacgg
     3901 gatgcggaac ggggacgccc attgcgccgc gttccattcg ctgcccggat gacggacaat
     3961 gccgctgccg tcaccatcag ggggaagaac gatgcgcaaa gcgctgtggt cctgcatcac
     4021 cgcatgttcg ctgctcgccg gctgcgccgc gatgcatccg cagcctcagc gggttctgaa
     4081 tcatgcgagc tgtcacgccg gcagcgcctg ccccgtcgac gtgaaggtca cgtgcgatca
     4141 cttctacggc tgcgacatct cggtcgaccc tgacctgatc gtcgtcgaag accgcggaca
     4201 gcgtaccgac atcgtgtggc ggctgtcggg cgacaccggc gcgcgcttcc cggccaacgg
     4261 cgtggttttc gacgacagcc tgctcaagtg cgagccacgc gccgacgggc gcgaattcgt
     4321 gtgcggcgac acgcctcccg gattcggtgt tttcaagtac cgggtcagcg tcacggttgc
     4381 gcagagcctg ttcgggccgc gcggcgcgct gtcgctcgat ccgtgggtcg tgaacaggta
     4441 gggccgacgc gacgcctcgc gccggctcgc gtcacgcggg tgccggttgc gcgcttgcgg
     4501 cgatccagcc gcgcacctcg gcgatcgccg cgtcgcggct gacgacgcgg cccgcagcct
     4561 cgatcgaccc gaacgccggc gcgccgagcg cagcgcgcgc ccggtcgatc agcggagcga
     4621 gaaacgcttc gtcggcggga tcgcgccgca gcccggtcgt ttccgccaac gcttcggcaa
     4681 cgccgaacag ccgcgcggcg cgcttccagt cgcctttcgc ggctgcccag cccgcggaca
     4741 cttccagcac gctctgcgtg gccggcgtcg agcggttttc gtcggcgatg gcgagcgcct
     4801 cgagcaggat cgccggaacg cgatcgctgc cgttgcgcgc gatcagggcc atggccaggt
     4861 tgagcagccc gaacgcgacg acttcccgat cgccggtctc gcgcgcgagt ccgacgacct
     4921 gttcatagag gggcagcgcc gcatcgagat cgttgcgtgc gcgatgcacc tgcgccatcg
     4981 cgttcagcgc cgaggcgaga tcgtgcttgt tgccctgcgc acgcacgagc acgagcgctt
     5041 cctcgagatg atgctgcgcc acggccgctt cgccgaggcc aaggcacgcc atcgcgagcg
     5101 gctgcagcgc ggatgcgatg cgcccgtcgt tgccgagctc gcgcgcgatc gcgaggctct
     5161 cctcgaggtg ccgccgccct tcgccgtagc gtcccatgta gcaggccatc tgcccgacgt
     5221 cgaacagcgc ccggcagcgc accacggacc ggtcgtcgcc ccgcgtgcgc gccagggcct
     5281 cgacgccgag ccggtaaccg agtcccggct cgccgcgatg tatccagtag aacttgagcg
     5341 tcgccagccg caacccgaga tcgacgccgt cctgcgccga tgcgcaccgg gcatgcgctg
     5401 ccagcaggtt ctcgcgctcg ccatcgagac gcgcgaacac ctcgccctgt cttggcccga
     5461 acagttccgg taccgcggat tccgcgacgg cgacatagtg gcgcaggtga ctgtcgcgca
     5521 gcacgttcgc ctcgccgctg ccttccagcc gctcgcgcgc gtactcgcgt acggtttcga
     5581 gcacccggta gcgcgcgccg tcggcgtcga gcacgacgag cgatttgtcg acgagcttcg
     5641 cgagcacgtc catgaccgcc gcgtccggca gcgctgcgtc cgacgcaatc gactcggcat
     5701 cctccatcgt gaacccgccg gcgaatacgg cgagccggcg gaacagtgtc cgctcgggtt
     5761 ccgagagcag ctcgtagctc cagtcgatca gcgcgcgcag cgtctgctgc cgcggaaggg
     5821 ccgtccggtc gccgccagtc agcagccgaa accggttgtc gaggcgtgcg gcgatcgtgg
     5881 cgatcggcag tacgcgagcg cgcgcggcgg cgagctcgat agcaagcgga atgccgtcga
     5941 gacgccggca gatttccgcg atgtcggtga tggtttgcgc gttcgcttcg aggccgggct
     6001 gcgacgccgc gatgcgatcg atgaaaagcc gcaccggatc cgattgcgcg agcgtcgtcg
     6061 cggcgtgcgg cgcgtcgcga tcggggacga gcatcggtgc aagcgggtag gcgcgttcac
     6121 cacgcacatt gagccgctcg cggctcgtcg cgaggatgcg cacgtccttc gtcgattgca
     6181 aaagctgcac cgcgaactcg gcgcatgcat cgatcagatg ctcgcagttg tcgagcacga
     6241 gcagaatcgc gcggtcctgg caatgccgga ccagcgcgtc ggtgatcgaa cggcccgcat
     6301 cctccttgac ggcaagcgcc gcagccgccg cttgcgcgac gagccgtgcg tcggcgagcg
     6361 aggcgaggtc cacgagccat gcaccgtcct ggaattcgtc gagcacgtcg gccgccacct
     6421 gcagcgacag ccgcgtcttg ccgaggccgc ccgtcccggt gatggtgacg agccgcgacg
     6481 tccgaagcag cgctccgatt tcaccgagct cgcgctcgcg tccgatgaag gacgtgagct
     6541 gttgcggcag gttgttcgga attgcctcga gggagcgcag cggcgggaac gtcgcccgca
     6601 gcgccgagtg cacgagctgg aacagacgct cgggcgttgc gaggtcgcgc aagcgcacga
     6661 cgccgagatc gagcagcgtc acgtcgcccg gcaggcggcc gccgaggagc tcggcgaccg
     6721 actgcgacac gaggatctgg ccgccgtgcg cggcgctcat gatgcgggcc gcccggttga
     6781 cggcggtgcc gaaataatcg ttgtcgcgat gctcgacgac gccggcatgc agtccgcatc
     6841 gcacgcgcag cgtgagaccg cccgttgcct gcggatcgtc gaggcgccgc gccaggtcga
     6901 cgaccgccat cacgccatcg agcggatcgc cgaagacggc gtggacgcca tcgccggtcg
     6961 tcttgaccag cgtgccctgc cgcgtctcga tggccgaccg tgcgagcgca tcgtggcggg
     7021 cgagtgcctc gcgcatgcgc tcgggctcct ggtcccacaa ccgggtgctg ccctcgatgt
     7081 cggtaaacag gaacgtcgtg accgcgggtt gttcttgcac gaatcgcccc ggatgtgccc
     7141 tttcgacact actttacggc agccgtcccg tacccaccag cgcggtatga tcgtgtgcgc
     7201 gtgcgcccgt agctcaattg gatagagcac cggccttcta agccggcggt tacaggttcg
     7261 agtcctgtcg ggcgtgccat gtcgccgcgc atcgccgcga tttgacttcc ccggaacaaa
     7321 ggcgttgtat acgagtcctc gtacgcagtg tcctgtcggc gaatccagcg tacgacactc
     7381 acaaggcgcc catcatccga ggtgccggaa ggccggagga gtccacgatg tcggcacgtc
     7441 cccgtgtcgc ttggtcatcg atggcggcgt tgcgggcatc gcatgcagcg cgcctgccgc
     7501 ttcattcgcc gaagactcga ttcgcctcgg cacacgcgct caacgcattc agcggcaagc
     7561 cgcccaacat tgcgccctag cggttgatcg cgcggcgtgg ccgccgcgat ctcgcggttc
     7621 gcctccgccc cgtagcaggc ggctccgccg tcggcgtggt ggtgcggcgc tgtcccatgt
     7681 cccaacttcc ggtgtccgac tcaatggtga gcggaccgga cttctcccaa ggagatgcaa
     7741 gatggctgaa caaaaatcaa aaatgccctt ccggcgcgct cgcgagttcg agaagcgcaa
     7801 gctcctgcag gtgagcatcg cgagcgcgtt ggcggccggg atggtgatcg cagcttcggc
     7861 ggacgcacgc acgacgagca ttcagatcac atcgcaggga atcgctttcg gcgggtacgc
     7921 ctttcccggc atcgggcagt acgaaaagat caccggcatc gccaccggag aggtcgatcc
     7981 caacgatccg cggaacgccg tgatcaccga cattgcgctc gcgccgcgaa acgcgaacgg
     8041 caacgtcgtc tatcagcaca atttctacat cctgaagccg gtcgatccga gcaagggcaa
     8101 tcaccaggtg atgtacgagc cgccgaaccg cggcggcaag acctatgcat cgctcaatcg
     8161 aacgccggtc ggcggaaatc tcaccaacga tcccggcgcg attaccgatg caacgctgct
     8221 cgcgggctcg ttcctgtggt cgcgcggcta cacgacggtg tggagtggct gggagaacaa
     8281 cctcggaccg ctcggcggtc tgacggccac ggcggtgctg ccggttgcga ccaatggcgg
     8341 cgaaacgatc accgggccgg cgtacgacta catcgtcaat ccgccgggcg gaacgtatgc
     8401 gcttacctat ccggccgcat ccggcaccaa gtctcccgtc gccttgctga cgcatcgcgt
     8461 gcatctcaac gatccgccgg tcgcggttcc cgattcgggc tgggattaca ccgacgcaac
     8521 ccgcacggcg atcaggctga ccggttcgag cttcatcacg aacgacatct acgagttctc
     8581 gtacaccgcg atgaatccga cggtcaacgg ccttggcttc gctgcggtac gcgatttcaa
     8641 ttcgttcatc cgctacgaca cgactgccgc caatccgctt gcgggcgacg tgacgcgcat
     8701 ctataccgag atctcgtcgc agcctgggcg tctgctcaac gacttcacca acctcgggtt
     8761 caacgaggac gaaagcggcc gcaaggtcat cgatggaatg atgcagtgga tcgcggcggg
     8821 tgatggcatc aacatgaact accgctggtc gcaaaccaag cggacgaacc gcaaccggca
     8881 ggagctcctc tttctcgaag gcaagttccc gttcgcgaac gtcgagacca ccgatcccca
     8941 tacgggcaag accgacagcc gcttcgcgcg ctgcgaaaaa tcgaacacgt gtcctgtcgc
     9001 ggcggaaatc tattcggcga acgaatactg ggtcaagggc gcatcgctgc tgcataccga
     9061 tcctgcggga acggtcgatc tgcccgattc cccgtatgcg cgcgactatt tcatctcgag
     9121 tcatcagcac ggaacgggaa atgctgcatc gaggggcgcg tgccagcagt tccagaaccc
     9181 gctcaactcc gcgcccattc agcgcgcgct gtttctggcg ctcgacgact gggtgaacgg
     9241 caccgcgccg ccgccgagcc gtgtgccgaa gcttgccgac ggcacgctgg tccctccgct
     9301 cccacagtcg gccgtcggct tcccgcacat tcccggcgtg acgtacacgg gactcgagac
     9361 gacgcggtat ctgttcgact tcggcccgaa cttctacgag accggcgtcc cgacgatcaa
     9421 tccgccgcac gtttcggcgc cgtaccagga caacccggcg aacgggccga tctatccgac
     9481 cttcgtgccg aagaccgaca gcgatggcaa cgacatcgcg ggggtgcgtc tgcccgacgt
     9541 cacggtgccg ctcgcgacgt acaccggttg ggcgttgcgc gccggcgtat gggcgaacga
     9601 cggatgcgaa ggctcggggc agtacatccc gttcccgagg acgaaggtcg atcgggtcgc
     9661 ctcgggcgat ccgcgaccct cggtggagga gcgctacaaa tcgttcggcc aatatcgcag
     9721 cgcggtcgtg aatgcggtcg acgacctcgt gaagaagcgg ctgatgttgt gcgaggatgc
     9781 cggcgaccag gtgcaccggc tggtcgacgc gggacttgcg gccggcgtgc cggcgccgac
     9841 gggacccgaa ccgatgtacc cggacgttcc gcactgcaac tagcgctcgc gttcgggcct
     9901 cgctgcaccc cgcaaaccca ccgtcgcgcc gcgacggtgg gttttttctg cgggatagcg
     9961 attacaatgg ccggcgcgcc ggacgcgaaa gcggagcagc acataacagc agcggtcggg
    10021 agcggcgcgt attcccgatc gatcgattca atggaggaga caccgatgaa aagcattacg
    10081 cctgtcggca tgctggccgc gcttgcgctg gcactcgcca gcggctcgct cgcggccgcg
    10141 ccggtcgtcc tgcgtgcatc ccatcagttc cccggcggca aaggcgacgt ccgcgacgag
    10201 atggtgcaga tcatcgcgcg cgatgcgaag gcagcgggcg tcgatctcga cgtccaggtc
    10261 tatcctggag cggcgctgtt caagccgaac gagcaatgga acgcgctcgt caacggacag
    10321 ctcgacatct cgtcgttccc gctcgactat gcgagcggca aggtgcgcgc gttcggcgcg
    10381 acgctgatgc cggggctcgt gcgcaaccac gagcgtgcag cccgcctcaa cgattccccg
    10441 ttcatgaagg agatcaaggc gaaaatcgag aaggcgggcg tcgtcgtgct gtccgactcg
    10501 tggctcgccg gtgcggttgc atcgaagaag ggctgcattc gcacgccggc cgacatgcag
    10561 ggcctcaaga tccgctcggc cggtccgaca ttcgcagaaa tgtggcaggc ggccggcgcc
    10621 tcgatcgtct cggtgccatc gaacgaggtc tacaacgcgt tgcagaccgg tgtggccgat
    10681 gcgacggaca cgagcacgag cagcttcgtg tcgttccgca tctacgagca ggtcaaatgc
    10741 atcacggcgc ccggggagaa cgcgctgtgg ttcatgtacg agccggtgct gatgtcgaag
    10801 aagagcttcg accggctcga caagaagcag caggcggtgt tgctcgccgc cggcaagaag
    10861 gccgaggcgt tctatgcgaa ggagggcaag aagctcgacg acgagatggt caaggcgttc
    10921 aaggaccaca acgtggaagt ggtgacgatg acgccggccg aatacgacca gtggatcgcc
    10981 atcgccaaga agagctcgta tgccgatttc gcgaaggaag tgcccgacgg caagaagctg
    11041 atcgacgacg cgttgtcggt caagtaaccc ggcgaaggtg ccggtgcgcg gcggccccgc
    11101 cttcgcaccg gcgtccagcg atttgaaata cctcccaagc gacggcatgc tcgaccgctt
    11161 cgcgcgcgtc gtcagggcgt tgtcgcagct ctgcggtttc atcgccgcag ggctcgtggc
    11221 gctcgccgtc gtgatcgtct gcgagatggt gttcgtccgc tatgtgctga accagagcac
    11281 gatctggcag accgacttca tcacctactg catcaccgcg gcgacgttca tcggcagccc
    11341 gtacgtgctg atgacgcgcg ggcacgtcaa cgtgaacgtg ctgccgctgc atctcgcgcc
    11401 gcgcggccgc ttcgcgctgg cgctcttcgc cgcattgacg tcggccgcct tctgcgtcgt
    11461 catggccgtg atga
//
LOCUS       DASSVX010000367         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_366174, whole genome shotgun sequence.
ACCESSION   DASSVX010000367 DASSVX010000000
VERSION     DASSVX010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366174"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VFO53_15645"
     CDS             complement(<1..1032)
                     /locus_tag="VFO53_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET8877761.1"
                     /translation="MELAGWIEKQSRLSAELMSRAISATDLRRVRAQFGQTIVPARGS
                     VLASPAIADWNPEPDYFFHWVRDSAIVMRTVAELAMHAADETERRRWTTCFEDYVTFS
                     LRLARLDVSSNESHAARTEAAFRKFLRADDDIRALGADGLLGEPRFNPDGTLDVLGWS
                     RPQYDGPALRALACLHFREFAGASAELDALLALDLDFTARHAGEACIGPWEEPEENTH
                     HYYVALVQLGALVHGREFVQGADAEARLRTALERHRSVQYDVYAAIWPSRGGTREDLI
                     DSAVLLGVLDADLADGPHSVADPRVWQTLAALEGLFAREFPINRGRTAPALGRSRADR
                     YFGGGAWYVT"
     gene            1213..>2491
                     /locus_tag="VFO53_15650"
     CDS             1213..>2491
                     /locus_tag="VFO53_15650"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HET8877762.1"
                     /translation="MKHRQHSDSEGAPARSVATLLDSLRDRADASADPPPLPLFQPPS
                     IAYEGRSAAAESLALIRILWRSRHRAVVAWLAGGILLMLIGNMFGQIRLNDWNGAFFD
                     AIAKRNVAELGHQLLIFGGIVAFLLSLVVGQTWMQQMLKVRLREWLTRHLLDDWLGRG
                     RAYRLGLAGELGVNPDQRIEEDTRKLTDLSAGLAIGLLQAGILLISFIGILWSLSSHM
                     VFVVQGHHFSIPGYMVWCAIIYALIGSLLTWRVGLPLIGLNAQLYSREAELRFALVRV
                     SESAESIALHHGERDERRQLEGNVDRVIATMSKLTGAASRLTWITSGYGWLAIIVPTL
                     VASPGYFSGTLTLGGLMVVVGAFNQVQASLRWFVDQFPAVADWRATLHRVSGFKAAMD
                     RLDRSDEAVDHIRMARHPHGKLAFDEVSVLFADG"
BASE COUNT          420 a          857 c          796 g          418 t
ORIGIN      
        1 cgtcacatac caggcgccgc cgccgaaata gcgatcggcg cgcgaacgtc cgagcgccgg
       61 cgcagtgcga ccgcgattga tcgggaactc gcgcgcgaaa aggccctcca gcgcggcgag
      121 cgtctgccac acgcgcggat cggcgaccga atggggcccg tcggcgagat cggcgtcgag
      181 gacgccgagc agcaccgcgg aatcgatgag atcttcacgc gtcccgcccc tggacggcca
      241 gatcgcggca tagacgtcgt actgcacaga ccggtgccgt tcgagcgccg tgcgcaaccg
      301 cgcctcggca tcagcgccct gcacgaactc gcgcccatgc acgagcgcgc cgagctgcac
      361 cagcgcgacg tagtaatggt gcgtgttctc ttccggctct tcccacggac cgatgcaggc
      421 ctcgcccgcg tgtcgcgcag tgaaatcgag atcgagcgcg agcagcgcgt cgagctccgc
      481 gctcgcaccg gcaaattcgc gaaagtgaag gcaggcgagc gcgcgcagcg cgggcccgtc
      541 atattgcggc cgcgaccagc cgaggacgtc gagcgtgccg tctgggttga agcgcggctc
      601 gccgagcagt ccgtcggcgc caagcgcccg gatgtcgtca tcggcccgca ggaatttacg
      661 gaacgcggct tcggtccgcg cggcgtgtga ctcgttgctg ctcacatcga gccgcgcgag
      721 gcgcaggctg aatgtgacgt agtcttcgaa acacgtcgtc cagcggcgac gctcggtttc
      781 atccgcggca tgcatcgcca gttccgcgac cgtgcgcatg acgatcgcgg aatcgcgaac
      841 ccaatgaaag aaataatccg gttccggatt ccagtcggcg atcgcgggcg acgccagcac
      901 ggaaccgcgc gccggaacga tggtctggcc gaattgcgcg cgaacacgcc gcaaatccgt
      961 cgccgaaatt gcgcgcgaca tcaattcggc gctcaatcgc gactgttttt cgatccaacc
     1021 cgcaagttcc attttaaccg ccgagactca cacagtgcca attttgcatc gctcgcgcac
     1081 ctgcgacaaa gcagaacaca gagttttctt gcaatccgac cgcgcagaca caaatcttcc
     1141 actcgacgct gcgagtcgtg atgtcacgac cgcgcccgcc ggtcgggggg aggccggcaa
     1201 cacggactag acgtgaaaca ccggcaacat tccgacagcg agggcgcgcc cgcgcgctct
     1261 gtcgccacgc tcctggattc gcttcgcgat cgcgccgacg cgtcggccga cccgccgccc
     1321 ctgccattgt tccagccgcc gagcatcgcc tacgagggcc gtagcgccgc cgccgaaagc
     1381 ctcgcgctga tccggatctt gtggcgcagc cgccatcgcg ccgtcgtcgc atggcttgcc
     1441 ggcggcatcc tcctcatgct gatcggcaac atgttcggcc agatccggct gaacgactgg
     1501 aacggagcgt tcttcgacgc catcgccaag cgcaacgtcg ccgaactcgg tcatcagctc
     1561 ctgatcttcg gcggcatcgt cgcgtttctc ctgtcgctcg tcgtcggcca gacctggatg
     1621 cagcagatgc tgaaggtccg gctgcgcgaa tggctgacgc ggcatctgct cgacgactgg
     1681 ctggggcgcg ggcgcgccta ccggctcgga cttgccggcg aactcggcgt caatcccgac
     1741 cagcgcatcg aggaggacac gcgcaagctc accgatctgt cggctggcct cgcgatcggg
     1801 ctgctccagg cgggcatctt gctgatctcc ttcatcggca tcctatggtc gctgtcgagc
     1861 cacatggtct tcgtcgttca gggccatcac ttctcgattc ccggctacat ggtctggtgc
     1921 gcgatcatct atgcgctgat cggttcgctg ctgacatggc gcgttggcct gccgctgatc
     1981 gggctcaacg cgcagctata ttcgcgcgag gcggagctgc gcttcgcgct cgtacgcgtc
     2041 agcgagagcg ccgagagcat cgcactgcat cacggcgagc gcgacgagcg gcgccagctc
     2101 gagggcaatg tcgaccgcgt catcgccacg atgagcaaat tgaccggcgc cgcatcgcgg
     2161 ctgacctgga tcacctcggg ctacggctgg ctcgccatca tcgtgccgac gctcgtcgcc
     2221 tcgcccggct atttcagcgg cacgctgacg ctcggcgggc tgatggtggt ggtcggcgcc
     2281 ttcaatcagg tgcaggcgtc gctgcgctgg ttcgtcgacc agttccccgc ggttgccgac
     2341 tggcgcgcca cgctccatcg cgtcagcggc ttcaaggcgg cgatggaccg gctcgaccgc
     2401 agcgatgagg cggtcgatca catccgcatg gcgcgccatc cgcacggcaa gctcgccttc
     2461 gacgaggtga gcgtgttgtt tgcggacggc c
//
LOCUS       DASSVX010000368         4483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1516250, whole genome shotgun sequence.
ACCESSION   DASSVX010000368 DASSVX010000000
VERSION     DASSVX010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516250"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFO53_15655"
     CDS             <1..449
                     /locus_tag="VFO53_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET8877763.1"
                     /translation="RREAALAMLASVGLRPEHYARYPHMFSGGQRQRIAVARALMLNP
                     RLVVADEPIAALDVSTQAQVLNLLADLQQQLRVAYLFIAHNLQVVRVIADDILVMYRG
                     KAVEQGPAEAIFSSPCHPYTQALLASTPSLARRRSHARSIRGNVTR"
     gene            496..1692
                     /locus_tag="VFO53_15660"
     CDS             496..1692
                     /locus_tag="VFO53_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HET8877764.1"
                     /translation="MMKTNPVEHIGRWADDFTRIRRDLHAHPELGFEEHRTARLVCDQ
                     LASFGIAHHAGVGKTGIVAVIEGRSDRQGRRVGLRADMDALPLQEENDTAWRSRYDGR
                     MHGCGHDGHTTMLLAAARYLHETRNFDGIAFLIFQPGEEGYAGAKAMLDDGLFERFPA
                     ERVFALHNWPALPAGRIGITPGPAMAAADRIEIVIDGRGGHGAHPHIAVDPVLVAGHI
                     ITAVQSIVSRNVSPLDTAVISLCAMQAGNPGAMSVIPAHARLVGTVRTFRPKTQDMIE
                     ARLTELVGSIAAAFGARATLTYERVYPPTINHDADAMFAADVAETLIGRENVVRNLDP
                     SMGAEDFSFMLQAKPGAFARLGQGGAEHGAFLHSSRYDFNDGVIALGAGYLAALAEQA
                     MPLAAA"
     gene            1729..2814
                     /locus_tag="VFO53_15665"
     CDS             1729..2814
                     /locus_tag="VFO53_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HET8877765.1"
                     /translation="MPDIASAFAATYAAAREKFAQAARRQGLAVVSRVHPRVAGAQGE
                     ELAIDFALLGDAGARSLLVVLSGVHGVEGFCGAGCQVALLQDETVVAAVRHSGVAVLL
                     CHAVNPYGFSHLRRTNEDNIDINRNFRDFAAPQAPNPAYASLHPVIVPPEWPPTPGNA
                     AIVDDYVARHGAARLQAAVSGGQCDFPDGLFYAGRAPAWSNTTLREALGEHGQARGRI
                     GWIDIHSGLGPWGLGEKIYAGPDDASMLARARAWYGCDVTSYYDATSTSASLTGVSFH
                     AALDACPQAEFTGIGIEYGTRPLEEVFDALRAEQWLANHPDTGEPARGAIKRQLRNAF
                     HDDTDAWKMIVYGQARVAVLQALRALT"
     gene            2826..3200
                     /locus_tag="VFO53_15670"
     CDS             2826..3200
                     /locus_tag="VFO53_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877766.1"
                     /translation="MPFARWCVAAAGLIVAGCQTWGPTWSELSGARYTRINPDRAQAI
                     LVSAGDESIGSVTPFRVAPGKSRVVVQSPVHNRFRGSEKDMMLDIAPCKRYYINAQFA
                     NPVSPDWTPVVDYVETIAGCRA"
     gene            3319..>4483
                     /locus_tag="VFO53_15675"
     CDS             3319..>4483
                     /locus_tag="VFO53_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8877767.1"
                     /translation="MRLTQIKLSGFKSFVDPTAIGTPGQLVGIVGPNGCGKSNVIDAV
                     RWVLGESKASALRGESMQDVIFNGAGERKPVGRASVELHFDNSQGRIGGQWGQYAELS
                     IKRMLTRDGDSTYYINNIPVRRRDIHDLFLGTGLGPRAYAIIEQGMISRVIEAKPEEL
                     RVFLEEAAGVSRYKERRRETESRLADTRGNLSRVEDIRQELGNQLAKLEEQAKIAGQY
                     HELEARHRQAQHLLWHAKAQESMRARERCSADIAALTVALEALQADVRQAEARLVQLR
                     DQHFAAGDALHERQGAFYAANAEVTRLEQQLAFARESETRLTEQLAQLTEAIGALGGQ
                     QAALDAEQADAERALQDALAARERTQADEAQAQSGLPPRESAVADASRILAEVQ"
BASE COUNT          731 a         1515 c         1534 g          703 t
ORIGIN      
        1 agcgccgcga ggcggcgctc gcgatgcttg cgagcgtcgg cctgcgtccc gagcattacg
       61 cgcgctatcc gcatatgttc tcgggcgggc agcggcagcg gatcgccgtg gcgcgcgcgt
      121 tgatgctgaa cccgcgcctc gtcgtcgccg atgagccgat cgcggcactc gacgtgtcga
      181 cgcaggcaca ggtgctgaac ctgctggccg acctgcagca gcaactgcgc gttgcctacc
      241 tgttcatcgc ccacaacctg caggtagtac gtgtgattgc cgacgacatc ctggtgatgt
      301 atcgcggcaa ggccgtcgag cagggaccgg cggaggcaat tttttccagt ccatgccatc
      361 catatacaca ggcgttgctc gcgagcacgc caagcctcgc ccggcgtcgt tcacacgcgc
      421 gcagcatccg cggcaacgtt acgcgatgat tcgcattgac gctcgcggat gcattgggca
      481 tgatcgcggc ccatcatcat gaaaacgaat ccggtcgagc acatcggccg ctgggcggac
      541 gacttcaccc gcatccggcg cgacctgcac gcgcatcccg agctcggctt cgaggagcac
      601 cgcaccgcgc ggctcgtctg cgaccagctc gccagtttcg gcatcgcgca tcacgcgggc
      661 gtcggcaaga cgggcatcgt cgcggtgatc gagggacgca gcgaccggca ggggcgccgc
      721 gtcggcctgc gcgcggacat ggacgcactg ccgctgcagg aggaaaacga cacggcgtgg
      781 cgctcgcgct acgacgggcg catgcacggc tgcggccatg acggccatac gacgatgctg
      841 cttgccgccg cgcgctatct gcatgaaacc cgcaacttcg atggcatcgc gttcctgatc
      901 ttccagccgg gcgaggaggg ctatgccggt gcgaaggcga tgctcgacga cgggctgttc
      961 gagcgctttc cggcggagcg cgtgttcgca ttgcacaact ggccggcgct gcctgccgga
     1021 cgcatcggca tcacgccggg accggcgatg gccgcagcgg accgcatcga gatcgtcatc
     1081 gacggacgcg gcgggcacgg cgcgcacccg cacatcgccg tcgacccggt gctcgtcgcg
     1141 ggccatatca tcaccgcggt gcaatcgatc gtcagccgca acgtgagccc gctcgacacc
     1201 gcggtgatca gcctgtgcgc gatgcaggcg ggcaatccag gcgcgatgag cgtgattccg
     1261 gcgcacgcgc ggctcgtcgg caccgtgcgc acgttccggc cgaagacgca ggacatgatc
     1321 gaggcgcgcc tcactgagct cgtcggctcg atcgccgccg cgttcggcgc ccgcgcgacg
     1381 ctcacctacg agcgcgtgta tccaccgacg atcaaccacg atgccgacgc catgttcgca
     1441 gcggacgttg cggagacgct gatcgggcgc gagaacgtcg tgcgcaatct cgacccgtcg
     1501 atgggcgccg aggatttttc gttcatgctg caggcgaagc ccggcgcctt cgcacggctc
     1561 ggccagggcg gggccgagca cggcgcgttc ctgcacagca gccgctacga tttcaacgat
     1621 ggcgtcattg cgctcggcgc gggttatctc gcggcattgg ccgagcaggc gatgccactc
     1681 gcagcggcgt aggcgcgcag cgccgccagg gttgcgccga tttcgtcgat gcccgacatc
     1741 gcctccgcgt tcgccgcgac gtacgcggca gcgcgcgaaa aattcgcgca ggctgcgcgg
     1801 cgccagggac tcgccgtcgt ttcgcgcgtg catccgcggg tcgcgggcgc gcagggcgag
     1861 gagcttgcca tcgatttcgc gctgctcggc gacgccggcg cacggtcgct cctcgtcgtg
     1921 ctgtccggcg ttcacggcgt cgagggcttc tgcggcgccg gatgccaggt cgcgctgctg
     1981 caggatgaaa ccgtcgtcgc cgccgtgcgc cacagcggcg tcgcggtgct gttgtgccat
     2041 gcggtcaatc cgtacggttt ttcacatctt cggcgcacca acgaagacaa catcgacatc
     2101 aatcgcaact ttcgcgattt cgccgcgccg caggcgccga atccggcata cgcgagcctg
     2161 catccggtca tcgttccgcc cgagtggccg ccgacacccg gaaacgccgc gatcgtcgac
     2221 gattacgtcg cccgtcatgg cgccgcccgg ttgcaggcgg ccgtgagcgg cggtcagtgc
     2281 gacttccccg acgggttgtt ctatgccggc cgcgcgcccg catggagcaa tacgacgctg
     2341 cgggaagcgc tgggcgagca cggacaggca cgcgggcgga tcggctggat cgacattcac
     2401 tcggggctcg gtccctgggg ccttggcgag aagatctacg ccggccccga cgacgcgtcg
     2461 atgctcgcgc gcgccagggc ctggtacggc tgcgacgtga cgagctacta cgatgcgaca
     2521 tcgacatcgg cgtcgttgac cggcgtgtcg ttccatgcag cgctcgatgc ctgtccgcag
     2581 gccgaattca ccggcatcgg catcgaatac ggcacacggc cgctcgagga ggtgttcgac
     2641 gcgctgcgcg ccgagcagtg gcttgccaac catcccgaca ccggcgagcc ggcgcgcggc
     2701 gcgatcaagc gccagttgcg caacgcattc cacgacgaca ccgatgcgtg gaaaatgatc
     2761 gtctacggcc aggcgcgcgt ggccgtgctg caggcgctgc gcgcgttgac gtgaaaggag
     2821 gccgtatgcc gttcgcaaga tggtgcgttg ccgctgctgg cttgatcgtg gccggctgcc
     2881 agacgtgggg tccgacctgg agcgagcttt ccggggcgcg ctacacgcgg atcaatcccg
     2941 atcgtgcgca ggccatcctc gtttcggccg gggacgaatc gatcggcagc gtgacgccgt
     3001 tccgtgtggc gcccggaaag tctcgcgtcg tcgtgcagtc gcccgtgcac aaccggttcc
     3061 gcgggtccga gaaggacatg atgctcgaca tcgcgccgtg caagcgctat tacatcaatg
     3121 cgcagtttgc aaatccggtg agccccgact ggacgccggt ggtcgactac gtcgaaacga
     3181 tcgcaggctg cagggcttga gcggaagagg tcgcggcacg gaccgctcgt tccgctataa
     3241 ttccgcgact ttttcgccgc ccgtccctcg cctgccaggt cgagcggctc cgcagtccgc
     3301 tttcttccga aaattccctt gcgcctgacg cagatcaagc tctccggctt caagtcgttc
     3361 gtcgatccca cggcgatcgg cacaccgggc caactcgtcg gcatcgttgg acccaatggt
     3421 tgcggcaagt cgaacgtcat cgacgcggtg cgctgggtgc tcggcgaatc gaaggcttcc
     3481 gcgctgcgcg gcgagtcgat gcaggacgtc atcttcaacg gcgccggcga gcgcaagccg
     3541 gtcggccgcg cttccgttga attgcacttc gacaacagcc agggccgcat cggcggtcaa
     3601 tggggccagt acgccgagct gtcgatcaag cggatgctga cgcgcgacgg cgactcgacg
     3661 tactacatca acaacatccc ggtccgccgg cgcgacatcc acgatctgtt cctcggcacc
     3721 gggctcgggc cgcgcgcgta cgcgatcatc gagcagggaa tgatctcgcg cgtcatcgag
     3781 gcgaagcccg aggagctgcg cgtcttcctc gaagaggcgg cgggcgtgtc gcgatacaag
     3841 gagcggcgcc gcgaaaccga gagccggctc gccgacacgc gcggcaatct ttcccgggtc
     3901 gaggacatcc gccaggagct cggcaaccag cttgcgaagc tcgaggagca ggcgaagatc
     3961 gccgggcagt atcacgagct cgaagcgcgg catcgccagg cgcagcatct gctgtggcat
     4021 gcgaaggcgc aggaatcgat gcgggcgcgc gagcgctgca gtgccgacat cgcggcgctc
     4081 acggtggcgc tcgaagcgct gcaggccgac gtgcggcagg ccgaggcgcg gctcgtgcag
     4141 ttgcgcgatc agcacttcgc ggccggtgat gcgctgcacg agcggcaggg cgcgttttac
     4201 gcggccaatg ccgaggtgac gcgcctcgag caacagctcg cgtttgcgcg cgaaagcgag
     4261 acgcggctca ccgagcagct cgcgcagttg acggaagcca tcggcgcact cggcggccag
     4321 caggcggcac tcgacgccga gcaggccgat gccgagcgcg cactgcagga cgcgctcgcg
     4381 gcccgcgagc gaacgcaggc cgacgaggcg caggcgcaat cggggttgcc cccgcgcgag
     4441 tccgccgttg ccgacgcgtc gcgcatcctg gccgaggtcc agc
//
LOCUS       DASSVX010000369         7688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1599688, whole genome shotgun sequence.
ACCESSION   DASSVX010000369 DASSVX010000000
VERSION     DASSVX010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599688"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VFO53_15680"
     CDS             <1..1281
                     /locus_tag="VFO53_15680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET8877768.1"
                     /translation="ISIAGFVAASALCGIATSLPEIVLFRLLQGVFGASLVPLSQAIM
                     VDSYPMHERGKALAIWGMGVMVAPILGPTIGGWLTETLDWRWNFYINVPIGVLSFLLA
                     ARHVPDTPVKARGMDWWGLAFLAMFIGGLQYLLDRGQQEDWLSSASIRWSAATLVAGI
                     ACFVAHSMMSKRDALFNLSMFADRNFAAASFVGGAMGLSLYGGMLLQPVLFESVLQYP
                     TFDTGLAMMPRGIGSLISMLIVGRLVGRIGAKPLIYFGIATGFLGAWMMTHVGLDSTA
                     SALIVPLLLQGVGVGFVFVPLSAIAFATLPKALSAEAAGVYSLIRSIGSSIGISIVSV
                     SLSRGAQASWGALRSYVDPYRFEVQRYLDPLHLKAHGLGLALIAQQTALQAQMLGLLR
                     AFWVIVASFVVMVPLVMLLRPGRGGAPPHAAMAE"
     gene            complement(1232..2500)
                     /locus_tag="VFO53_15685"
     CDS             complement(1232..2500)
                     /locus_tag="VFO53_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8877769.1"
                     /translation="MLAQRPFVLFWFARVFAMLARQMVGVAVGWQMYALTGSALDLGF
                     VGLAQFVPSFFLVLVAGHVADRFDRRRILQACMLAESLAALGLCAGSVFGGIDPVTIF
                     ALVFVIGAARAFEMPTMQALLPALVPEPLLARAIAATASASQAAIIGGPALGGLLYIA
                     GPGIVYGASMSLFVLTGVIVHLVRPRHAPPRLTTPGVSSVLDGIRFIRARPAVLGAIS
                     LDMFAVLLGGATALLPIYARDILYTGPWGLGLLRSASAVGALGMALWLAHHPMRGHAG
                     RRMFAGVALFGAATIVFGISRSFTLSLAALIVLGAADMVSVVVRQSLVQLSTPDHMRG
                     RVSAVNALFIGTSNQLGEFESGLTAAWLGVVASVVAGGIGTLAIVVLWMRLFPALANV
                     QRLDSSLLANEIVPATRPSPRAAARRRDRA"
     gene            complement(2554..3123)
                     /gene="folE"
                     /locus_tag="VFO53_15690"
     CDS             complement(2554..3123)
                     /gene="folE"
                     /locus_tag="VFO53_15690"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET8877770.1"
                     /translation="MQVDIARIEKLTRELIEAIGEDPTREGLQKTPERVAKAIAFLTS
                     GYRTDLPAIINGAIFTQTTNSMVIVKNIEVYSLCEHHMLPFFGRCHIGYIPTGRVFGV
                     SKLARLVDMYARRLQLQERLTEQISQAVHDSIDARGVGVMIEARHLCMMMRGVEKQNS
                     EMVTSSVLGTFRESQATREEFLALIGSRA"
     gene            3319..4293
                     /locus_tag="VFO53_15695"
     CDS             3319..4293
                     /locus_tag="VFO53_15695"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET8877771.1"
                     /translation="MSRTADGGERRTTCPSCGRQVAAGARYCIQCGAEQSVPTPIAAV
                     AAAMARGQPREAANAAHADPARSILSDASPTDAAPAAATLTRAASVSAANSAAPTPPA
                     YDAGPPRARLAAVLIGCLVAVTIALAAVALWRGERGAAITAGESDAGATAASAAPSGA
                     SPRASTEAPPAPTVQTKSETASSPASAAGTNPAPGAPAPSTATPDVASGPVEIKALPP
                     HAPARRSPHRSAPESTASAPPTPPPAEPAAAPAPPPIAQARSAAPARATQRVADHWQH
                     LDDELSRCTREDFITRVICGQRARFRYCDGYWGKVPQCPGNPLPERGQ"
     gene            4455..5042
                     /locus_tag="VFO53_15700"
     CDS             4455..5042
                     /locus_tag="VFO53_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877772.1"
                     /translation="MTQIVAGVFDDEQAATSAAHELRNAGFGAADLDQFSLRPPGQHQ
                     GLPSGGDETADAKAKGGDRGAMKGAAVGTVVGAAAGLAATPLLGPIAVAGGAAAGAYA
                     GSLAGAVGRMGDDRPAPQLRPAGVMVAVNADTFDDKDVAIGVLHDHGARMIERADGAW
                     RDGKWADFDPVRPPDVLESHVAGAAPSPAAHRGAR"
     gene            5039..5197
                     /locus_tag="VFO53_15705"
     CDS             5039..5197
                     /locus_tag="VFO53_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877773.1"
                     /translation="MSVEVRARTRRPALRAIGPRRLQEGRWGYILAWLIGVPVPILIV
                     IYLLRGCT"
     gene            complement(5353..6129)
                     /locus_tag="VFO53_15710"
     CDS             complement(5353..6129)
                     /locus_tag="VFO53_15710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8877774.1"
                     /translation="MSPTAPANEARKSAIVTGAGSGIGRAVALALADAGYRVALAGRR
                     KDALDETVRAARTRNAELVPVVTDVGNPDSVARLFASVAERFGRLDLLFNNAGIGSPA
                     VGLEELPVETLRAVLDTNLLGPFLCTQHAIRMMKAQSPRGGRIINNGSISAHAPRPNS
                     APYTATKHAITGLTRSTSLDGRKYDIACGQIDIGNAATEMTARMSGGVPQASGETAVE
                     PRMDVAHVASAVLYMAGLPLDANVQFMTVMATKMPFVGRG"
     gene            complement(6126..>7688)
                     /locus_tag="VFO53_15715"
     CDS             complement(6126..>7688)
                     /locus_tag="VFO53_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012076967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET8877775.1"
                     /translation="GAIVEAEGNVVLLCRGAESIAIDYSVAPIRDRAGNSVGAVLVIQ
                     DMSRERQYAARLSNLASHDALTGLLNRREFEQRVRAIVEQREGPEGQHAVLYLDLDQF
                     KVVNDTSGHAAGDELLRQVGALLRPRLREGDVLARLGGDEFGVLLPHCPPAPALRIAE
                     ALRKAIIDFRFAWKNRSFTIGVSIGLVNLADGPHTLATVLSAADAACYLAKDKGRNRV
                     QVYRPEDSEVTLRRGEMEWVHRLHRALAEDRLCLYSQPMRAMHAPGPHVQHEELLVRL
                     IDEQNELIPPIAFIPAAERYHLMPSIDRWVIRTAFRVLAERRAAGASLGTYAINVSGA
                     SIGDDQFLDYVRESFARFRIPHASICFEITETTAVTSLSKAAEFIGALREPGCRFALD
                     DFGVGVSSFTYLKQLPVDFLKIDGSFVKNMLNDPVDAAMVEAIHRIGRVMGKQTIAES
                     VETAATLDALRSVGVDYAQGHAIAPPSMFAPPGARMRHVAERGRGEAARPVSSVQRAA
                     LVSRIGNDRSHR"
BASE COUNT         1195 a         2629 c         2717 g         1147 t
ORIGIN      
        1 atcagcatcg cgggcttcgt cgccgcgtcg gcgctgtgcg gcatcgcgac gtcgctgccg
       61 gagatcgtgc tgtttcgtct tctgcaaggc gtgttcggcg cctcgctcgt gccgctgtcg
      121 caggcgatca tggtcgacag ctacccgatg cacgagcgcg gcaaggcgct cgcgatctgg
      181 ggcatgggcg tgatggtcgc gccgatcctc ggcccgacga tcggcggctg gctgaccgaa
      241 acgctcgact ggcgctggaa tttctacatc aacgtgccga tcggcgtgct gtcgtttctc
      301 ctcgccgcac gacacgtgcc cgatacgccg gtcaaggcac gcggcatgga ctggtggggg
      361 ctcgcgtttc tcgcgatgtt catcggcgga ctgcagtatc tgctcgatcg cggccagcag
      421 gaggactggt tgtcgtcggc gtcgatccgc tggtcggcgg cgacgctcgt cgcgggcatt
      481 gcctgcttcg tcgcgcactc gatgatgtcg aagcgcgacg cgctcttcaa cctgtcgatg
      541 ttcgccgacc gcaatttcgc cgcggcgtcg ttcgtcggcg gtgcgatggg cctgtcgctg
      601 tacggtggga tgctgctgca accggtgctg ttcgaaagcg tgctgcagta cccgacgttc
      661 gacaccggcc tcgcgatgat gccgcgcggg atcggcagcc tcatcagcat gctgatcgtc
      721 gggcgcctgg tgggaaggat cggcgccaag ccgctcatct acttcggcat cgccaccggc
      781 ttcctcggtg cgtggatgat gacgcacgtc ggcctcgatt cgacggcgag cgcgctgatc
      841 gtgccgctcc tgctgcaggg cgttggtgtc ggcttcgtgt tcgtgccgct gtcggcgatc
      901 gcgtttgcga cgctgccgaa ggcgctgtcg gccgaagcgg ccggcgtcta cagcctgatc
      961 cgctcgatcg gatcgtcgat cggcatctcg atcgtcagcg tgtcgctgtc gcgcggtgcg
     1021 caggcgagct ggggcgcctt gcgaagctac gtcgatccgt atcgcttcga ggtgcagcga
     1081 tacctcgatc cgctgcacct caaggcgcac gggctcggcc tcgcgctgat cgcccagcaa
     1141 acggcgctgc aggcgcagat gctcggcctg ctgcgcgcgt tctgggtgat cgtcgcgagc
     1201 ttcgtcgtga tggtgccgct cgtgatgctg ctcaggcccg gtcgcggcgg cgcgccgccg
     1261 cacgcggcga tggccgagta gcgggcacga tctcgttcgc cagcagcgac gagtcgagtc
     1321 gttgcacgtt ggccagcgcc ggaaaaagac gcatccacag cacgacgatc gccagcgtgc
     1381 cgatgccgcc ggccaccacc gaggcaacca cgccgagcca cgccgcggtc agtcccgatt
     1441 cgaattcacc gagctgattc gacgtgccga tgaacagcgc gttgacggcg ctcacgcggc
     1501 cgcgcatgtg atcgggcgtc gaaagctgca cgagcgactg gcgcacgacc acgctcacca
     1561 tgtcggcggc gcccagcacg atgagcgcgg ccagcgacag cgtgaacgag cgcgaaatgc
     1621 cgaacacgat cgtcgccgcg ccgaacagcg cgacgccggc gaacatgcgc cggcccgcat
     1681 ggccgcgcat cggatggtgg gcgagccaca gcgccatgcc gagtgcgccc acggcgctcg
     1741 cggagcgcag caacccgagt ccccacggcc ccgtgtacag gatgtcgcgg gcgtagatcg
     1801 gcaacagcgc ggtcgccccg ccgagcagca ccgcgaacat gtcgagcgag atcgcgccga
     1861 gcacggcggg gcgggcgcga atgaagcgga tgccgtcgag taccgacgac acgccgggcg
     1921 tcgtcaaccg cggtggcgcg tgtcgcggcc gcaccaggtg cacgatgacg ccggtcagca
     1981 cgaacagcga catgctggcc ccgtagacga tgccggggcc cgcgatgtac aggaggccgc
     2041 cgagcgcggg accgccgatg atcgccgcct ggctcgccga cgccgtcgcg gcgatcgcgc
     2101 gggcgaggag cggttcgggg acgagcgccg gcagcaacgc ctgcatcgtc ggcatttcga
     2161 acgcgcgcgc cgcgccgatc acgaagacca gcgcgaagat cgtgacggga tcgattccgc
     2221 cgaatacgct gcccgcgcac agtccgagtg cggcgagcga ctcggcgagc atgcacgcct
     2281 gcaggatgcg ccgccggtcg aagcggtcgg cgacatggcc ggcgacgagg acgagaaaga
     2341 acgacgggac gaactgcgcg aggccaacga agccgaggtc gagcgcgctt ccggtcaacg
     2401 cgtacatctg ccagccgacg gcgacgccga ccatctggcg cgcgagcatt gcgaacacgc
     2461 gcgcgaacca gaacagcacg aacggccgct gcgcgagcag cgcgcggtcg cggtcgacgg
     2521 ctggcgtgcg tgcgccgcga cgcgcggcgc gcgtcaagcg cgcgagccga tcagcgcgag
     2581 gaattcctcg cgggtcgcct gcgactcgcg aaacgtgccg agcaccgacg acgtcaccat
     2641 ctccgaattc tgcttctcga cgccgcgcat catcatgcag aggtggcgcg cctcgatcat
     2701 caccccgacg ccgcgcgcgt cgatcgaatc atggaccgcc tgcgagatct gctcggtgag
     2761 gcgttcctgc agctgcaggc ggcgggcgta catgtcgacg agccgcgcca gcttggagac
     2821 gccgaataca cgccccgtcg ggatgtagcc gatgtggcag cgaccgaaaa acggcagcat
     2881 gtggtgctcg cacaggctgt agacctcgat gttcttcacg atgaccatgc tgttcgtcgt
     2941 ctgcgtgaag atcgccccgt tgatgatcgc aggaaggtcg gtgcgatagc ccgacgtgag
     3001 gaaggcgatc gccttcgcga cgcgctccgg cgtcttctgg aggccctcgc gggtgggatc
     3061 ctcaccgatc gcctcgatga gctcgcgcgt cagtttctcg atcctcgcta tgtcgacctg
     3121 catggcggcg tccggcggtg ggaagcgccg attatcgcgt gaaaccgcga agctaacgac
     3181 ggggccggcg ggagccctgc gagacgcggt gatcgcgaca gtcgacgacc ggtcgtcgtg
     3241 taaaaaatgc gcacgccttc aacccggcgt cattttcggg cacgatggat ggctttcagg
     3301 cacggtgcgg aacgcgaggt gagcagaacg gcggacggcg gagagcggcg cacgacctgt
     3361 ccgagttgcg gacggcaggt cgcggccggt gcgcgctatt gcatccagtg cggtgccgag
     3421 cagtcggtgc caacgcccat tgcggcggtt gccgcggcca tggcgcgcgg gcagccgcgc
     3481 gaagcggcga acgccgcgca cgccgatccg gcgcggtcga tcctgtccga cgcatcgccg
     3541 accgatgccg cgccggcggc tgcaacgctc acgagagcgg cgagcgtctc ggcggcgaac
     3601 tcggcggcgc cgaccccgcc cgcctacgat gccgggccgc cgcgcgcccg gctcgcggcg
     3661 gtgctgatcg gctgtctcgt cgccgtgacg atcgcgctcg ccgcagtcgc gctctggcgc
     3721 ggcgagcgtg gcgctgcgat cactgccggc gagtccgacg caggcgcgac ggccgccagt
     3781 gcggcgccgt ccggcgcaag tccccgggcg tcgaccgaag cgccgcccgc acccacggtg
     3841 cagaccaagt ccgaaacggc gtcgtcgcct gcgtctgctg ccgggacgaa cccggcaccc
     3901 ggcgcgccag cgccgtcgac cgcgacgccg gacgtcgcga gcggaccggt ggagatcaag
     3961 gcgctgccac cgcacgcgcc ggcgaggcgg tcacctcatc gaagcgcgcc tgaaagcacc
     4021 gcgtcggcac cgcccacccc gccgcccgca gagccggccg ccgcgccggc gcctccgccg
     4081 atcgcgcagg caaggagcgc cgcccccgca cgcgcgaccc agcgcgtcgc cgaccactgg
     4141 cagcaccttg acgacgagct gtcgcgctgt acgcgggagg acttcatcac ccgcgtcatc
     4201 tgcgggcagc gggcgcgctt ccgctactgc gacggctact ggggcaaggt gccccagtgc
     4261 cccggcaatc cgctccccga gcgcgggcag taagccgcca tcccgctgca gcgcggcatc
     4321 acggctcgcg ctgcttcacc gtaggtgcat ccttacacgg gattccgcgc cgagccgatc
     4381 ctcgtcggct ggcgatcgcg tctaacctga acggcatcgg tcccggtacg cgcgggacgc
     4441 cttcgggaga cacaatgacg cagatcgtgg caggcgtctt cgacgacgag caggcagcga
     4501 cttccgctgc acacgaattg cgaaacgccg ggtttggcgc agcggacctc gaccagttct
     4561 cgctgcgccc gcccggccag caccaggggt tgccgagtgg cggcgacgaa acggccgacg
     4621 cgaaggcgaa aggcggcgac aggggggcga tgaagggcgc cgcggtcggc acggtcgtcg
     4681 gcgccgcggc gggtcttgcg gcgacaccgc tactcggtcc gatcgccgtg gccgggggtg
     4741 ccgccgcggg ggcgtatgcc ggatcgctcg ccggtgcggt cgggcgcatg ggcgacgacc
     4801 ggcccgcgcc gcagctccgc cccgcgggcg tcatggtcgc ggtcaacgcc gacacgttcg
     4861 atgacaagga tgtcgcgatc ggggtgctgc acgaccatgg cgcacggatg atcgagcgcg
     4921 ccgacggtgc gtggcgcgac ggcaagtggg ctgacttcga tccggtgcgt ccgccggacg
     4981 tgctcgaatc acatgtcgcg ggcgccgcac cgagcccggc cgcacaccgg ggcgcgcgat
     5041 gagtgtcgaa gttcgcgccc gcacccggcg cccggcgctg cgggcgatcg ggccgcgccg
     5101 tttgcaggag ggacgctggg gttacatcct cgcgtggctg atcggcgtgc cggtgcccat
     5161 cctgatcgtg atctaccttc tgcgcggctg cacgtaggta ccaactgcaa aggcgtcaca
     5221 gaatcggagc gagcgcgccg agtgcgcgaa ggcccgcagc gagtcccgag acagtacgcg
     5281 aaaggtacgg cgagggacga gcgaggacac gacaaagcaa tcggtgcgcg cagcccgatt
     5341 atgtgacggc tttcagccgc ggccgacgaa cggcattttc gtcgccatca cggtcatgaa
     5401 ctgcacgttc gcgtcgagcg gcaggccggc catgtaaagc accgcgctcg ccacgtgcgc
     5461 cacgtccatc cgcggctcga cggcggtctc gccgctggct tgcggcacgc cgccgctcat
     5521 gcgcgccgtc atctcggttg ccgcattgcc gatgtcgatc tgcccgcagg cgatgtcgta
     5581 cttgcgtccg tcgagtgacg tcgatcgggt gaggcccgtg atcgcgtgct tcgtcgccgt
     5641 gtagggcgcc gagttcgggc gcggcgcgtg cgccgagatc gagccgttgt tgatgatgcg
     5701 tccgccgcgc ggactctgcg ccttcatcat ccggatcgcg tgctgcgtgc acaggaacgg
     5761 gccgagcagg ttcgtgtcga gcaccgcgcg caacgtttcg acgggcaact cctcgaggcc
     5821 cacggcgggc gagccgatgc ccgcgttgtt gaaaagcaga tcgaggcggc cgaaacgctc
     5881 ggcgacgctc gcgaagagcc gcgcaaccga gtcggggttg cccacgtcgg tgacgaccgg
     5941 gacgagctcg gcattgcgcg ttcgcgcggc acgcacggtt tcgtcgagcg catccttgcg
     6001 ccgtccggca agcgcgacac ggtatccggc atcggcgagc gcgagcgcga cggcccggcc
     6061 aatgccgctg ccggcgccgg tgacgatcgc gctcttgcgt gcttcgttcg ctggtgcggt
     6121 cgggctcatc gatgactcct gtcgttgccg atgcgtgaaa caagcgcggc gcgctggacc
     6181 gaagacaccg gccgggcggc ttcgccgcgg ccgcgctcgg cgacgtgccg catgcgcgcg
     6241 ccgggaggcg cgaacatcga cggcggcgca atcgcgtgac cttgcgcgta gtcgacgccg
     6301 acgctgcgca gcgcgtcgag cgttgccgca gtctccaccg actcggcgat cgtctgtttt
     6361 cccatcacgc ggccgatgcg atgaatggcc tcgaccatcg ccgcgtcgac cggatcgttg
     6421 agcatgttct tgacgaagct gccgtcgatc ttcaggaaat cgacgggcaa ctgcttcagg
     6481 tacgtgaacg acgacacgcc gacgccgaaa tcgtcgagcg cgaagcggca gcccggttcg
     6541 cgcagcgccc cgatgaattc ggccgccttc gacaggctcg tgaccgcggt cgtctcggtg
     6601 atctcgaagc agatcgacgc gtgcgggatg cgaaagcgcg cgaagctttc gcgaacatag
     6661 tcgagaaact ggtcgtcgcc gatcgaggcg ccggagacgt tgatcgcgta cgtgccgagg
     6721 ctcgcgccgg cggcgcgacg ctcggcgagc acgcgaaacg ccgtgcggat cacccagcgg
     6781 tcgatcgacg gcatcaggtg atagcgctcg gcggcgggaa tgaacgcaat cggcgggatc
     6841 agctcgttct gctcgtcgat cagccgcacc agcagctcct cgtgctgcac gtgcggaccc
     6901 ggggcatgca tcgcgcgcat cggttgcgag tagaggcaca gccgatcctc ggccagcgcg
     6961 cgatgcagcc gatgcaccca ttccatctcg ccgcggcgca gcgtcacctc gctgtcctcg
     7021 gggcggtaga cctgcacgcg gtttcggccc ttgtccttgg cgaggtagca cgcggcgtcg
     7081 gccgccgaga gcacggtcgc gagcgtgtgc ggtccgtcgg cgagattgac gagcccgatg
     7141 ctgacgccga tcgtgaacga gcggttcttc catgcgaacc ggaagtcgat gatggccttg
     7201 cgcagcgctt cggcgatgcg cagcgccgga gcaggcggac aatggggcag cagcacgcca
     7261 aattcgtcgc cgccgaggcg cgcgagcacg tcgccttcgc gcaggcgcgg ccgcagcagc
     7321 gcaccgacct gtcgcagcag ctcgtcgccg gcggcatgcc cgctcgtgtc gttcaccacc
     7381 ttgaactgat cgaggtcgag gtagagcacc gcgtgctggc cctccggtcc ctcgcgctgc
     7441 tcgacgatcg cgcgcacgcg ctgctcgaat tcgcgccggt tgagaagtcc ggtgagcgcg
     7501 tcgtggctcg cgaggttgga aagccgcgcc gcatactgcc gctcacggct catgtcctgg
     7561 atcaccagca cggcgcccac gctgttgcct gcacgatccc ggatcggggc gacgctgtag
     7621 tcgatcgcga tcgactcggc gccgcggcac agcagcacga cgttgccctc cgcctcgacg
     7681 atggcacc
//
LOCUS       DASSVX010000370         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1032849, whole genome shotgun sequence.
ACCESSION   DASSVX010000370 DASSVX010000000
VERSION     DASSVX010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032849"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFO53_15720"
     CDS             complement(<1..294)
                     /locus_tag="VFO53_15720"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET8877776.1"
                     /translation="MSTPTVLRVAAVQMSSGEDIAANLATAAALIDRAASEGAALVLL
                     PENFGLMARHARDKVAARERDGDGPQQAFLARMARLHRQYLIGGSVPLESDDPS"
     gene            complement(311..>3239)
                     /locus_tag="VFO53_15725"
     CDS             complement(311..>3239)
                     /locus_tag="VFO53_15725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HET8877777.1"
                     /translation="ANGLAFVLPDGSRFGPTDLALDLDEATPAAAAGGRLAVGEIELQ
                     PLAAIASHLPLPEAVRRDIVRFAPHGSVRDAHVEWTGELAAPQRYLVAGNVSRFAVAS
                     HDGLPGVAGIAGSIEMDQRGGRVHVAGNASTLTLPRIFADPLVFDSITGDVLWQTEAG
                     TTRVQWKGVGFANADLAGTTDGTWSSGPDGPGSVDITGRLTRANLASTYRYVPAVAPA
                     TLREWLQRALVKGSTGEARLALAGDLAFFPFAQGKQGRFELDVKAHDVTLAYADRWTP
                     ITDAAGDVRVDGAHVAVDASGAHMNGVEIGTTRAEIANWRDARPVLRIKGTARGPTPQ
                     FLAFVAASPIAEWIDHVTDGITASGDGELALEFALPLHDPGQTSVTGDYRFASSAVQL
                     APAVQLAEAAGALAFDEHGIRASAVTAQAFGGPVTLALTAERGHLRVDARGSADVERA
                     RTAFDAPALARVSGNTDWQLALDARGGQMAWTLQSSLAGATVDLPAPIGKVASETRPL
                     RVERRATKPQEDTISVDYGRIARVLIHRQTGGTTANVDRALVLVGKAIGDATSPERAG
                     VWIRADVAAIDVDAWLAVDPMRGDGAESTASARTPAIDGVDLQAASATALGRSFGNVK
                     ATARRRDGDWRVVLDGSEIAGNATWRSATIAQPYGRISARLTRLSLPPATQGIDAATT
                     TGASDPNHWAAVDLVADSVIRKGRALGKLELVAQPSGADWQITKLSLANDAGRIDAHG
                     SWRDASTRSQTQLDVAIDVREAGAFLGRFGWPDAVKAAPTRIEGQVSWAGSPSDFDYP
                     SLAGRLTLHTGAGQFTKLEPGMGRLLGVLSLQALPRRVSLDFRDVFSEGFAFDTITGD
                     VRIDRGVMHTGNLRLSGPAAGVDIAGEVDLAHETQKLDVRVQPSLSTGVSAGAAALFI
                     ANPLLGAAIGAGTLLAQKMLNNPFDQLFSYRYTVSGSFDDPVVARANARAASAESSSA
                     TIR"
BASE COUNT          485 a         1150 c         1076 g          528 t
ORIGIN      
        1 cgacgggtcg tcgctttcga gagggacgct tccgccgatc aggtactggc gatgcagacg
       61 tgccatccgc gcgagaaacg cctgctgcgg gccgtccccg tcgcgctcgc gtgcggcaac
      121 cttgtcgcgc gcgtgacgcg ccatcaatcc gaagttctcg ggcaacagca cgagtgccgc
      181 gccttcgctc gccgccctgt cgatcaatgc cgcggccgtg gcgaggttgg cagcgatgtc
      241 ttcgccggag ctcatctgga ccgccgcgac gcgcagcacg gttggcgtgc tcatccttcg
      301 cctccttgca tcatcgaatc gttgccgacg acgactcggc actcgcggca cgtgcattcg
      361 cccgcgccac gacgggatcg tcaaagctgc cggaaaccgt gtaccggtag ctgaagagct
      421 ggtcgaacgg attgttcagc attttctgcg cgagcagcgt tcccgcgccg atcgcagcac
      481 ccagcagcgg gttggcgatg aagagcgccg ccgcgcccgc cgagacccct gtcgacagcg
      541 acggctgcac gcgcacgtcg agcttttgcg tctcgtgcgc gagatcgacc tctccggcaa
      601 tgtcgacccc cgcggcgggg cccgaaaggc gcaggttgcc ggtgtgcatg acgccgcggt
      661 cgatgcgcac gtcaccggtg atcgtatcga aggcgaatcc ctcgctgaat acatcgcgaa
      721 agtcgagcga gacccgccgc ggcaacgcct gcagcgacag cacgccgaga agccgcccca
      781 tgccgggctc gagtttggtg aattgtcctg cgcccgtgtg cagcgtcaga cgcccggcga
      841 gtgacggata gtcgaagtcg ctcggtgagc cggcccatga aacctgccct tcgatcctcg
      901 tcggcgccgc cttcaccgcg tcgggccagc cgaaccggcc gagaaaggcg cctgcctcgc
      961 gcacgtcgat cgccacatcg agttgcgtct gcgaacgcgt gcttgcatcg cgccacgagc
     1021 catgggcgtc gatccggccg gcatcgtttg cgagcgagag cttcgtgatc tgccaatcgg
     1081 cgcccgaggg ctgcgcaacg agctccagct tcccgagcgc cctgcccttc ctgatcacgc
     1141 tatcggcgac gagatcgacg gcggcccaat ggttggggtc gctcgcgcca gtcgtcgtgg
     1201 cggcatcgat accttgcgtt gcgggcggca ggctcaagcg cgtcaagcgc gcgctgatgc
     1261 gaccgtacgg ctgcgcgatc gttgcacttc gccatgtggc attgccggcg atttcgcttc
     1321 cgtccagcac cacgcgccag tcgccatcgc ggcggcgcgc cgtcgccttg acgttgccga
     1381 agctgcgccc gagcgccgtg gcgcttgctg cctgcaagtc gacgccgtcg atcgcgggcg
     1441 tccgtgccga tgccgtcgat tccgctccat cgccacgcat cggatcgacc gcgagccatg
     1501 catcgacgtc gatcgccgcc acatcggcgc ggatccatac gccggcacgc tcgggcgacg
     1561 tcgcgtcgcc gatggctttg ccgaccagaa cgagtgcgcg atcgacgttc gcggtggtgc
     1621 cgccggtctg gcgatgaatc agcacgcgcg cgatgcggcc gtagtcgacg ctgatcgtgt
     1681 cctcctgcgg cttggttgca cgccgctcga cgcgcagcgg ccgcgtttcg ctcgcgacct
     1741 tcccgatcgg ggccggcagg tcgacggtgg cgccggcgag cgacgattgc agcgtccatg
     1801 ccatctggcc gccccgtgca tcgagcgcga gctgccagtc ggtgttaccc gacacgcgtg
     1861 cgagcgcggg tgcatcgaac gccgtacgcg cccgttcgac gtcggcgctg cctcgcgcgt
     1921 cgacgcgcag gtggccgcgc tcggcggtaa gcgcgagcgt gactggaccg ccgaatgcct
     1981 gcgccgtcac tgcgcttgcg cgaatgccgt gctcgtcgaa tgcgagtgcg cccgccgcct
     2041 ccgcgagctg aacggccggc gcgagttgaa ccgcgctcga cgcaaatcga taatcgccgg
     2101 tgacgctcgt ctgtcccgga tcgtgaagcg gcagagcaaa ttccagcgca agctcgccgt
     2161 cgccgcttgc ggtaatgccg tcggtgacgt gatcgatcca ttcggcaatc gggctcgccg
     2221 cgacgaacgc cagaaattgc ggcgtgggtc cgcgcgccgt ccccttgatt cgcagcaccg
     2281 gccgggcgtc gcgccagttg gcgatttcgg cgcgcgtcgt accaatctcg acaccgttca
     2341 tgtgcgcgcc cgatgcgtcg accgcgacat gcgcaccatc gacgcgaaca tcccccgccg
     2401 catcggtgat cggcgtccag cgatcggcat aggcaagcgt gacgtcatgc gccttgacgt
     2461 cgagctcgaa gcggccctgc ttgccctgcg cgaacgggaa gaaggcaagg tctccggcga
     2521 gcgccagccg cgcttcaccc gtgctgccct tgaccagcgc acgctgcagc cactcgcgca
     2581 aggttgccgg cgccacggcc ggcacgtagc gatacgtgct tgcgagattt gcgcgcgtca
     2641 gtcgcccggt gatgtcgacg ctgcccgggc cgtcgggtcc cgaactccac gttccatccg
     2701 tcgtgcccgc cagatccgcg tttgcaaagc ctacgccctt ccattgcacc cgcgtcgtgc
     2761 cggcttccgt ttgccagagg acgtcgccgg tgatgctgtc gaacacgagc ggatcggcaa
     2821 agattcgcgg caaggtgagc gtcgatgcgt tgccggcaac gtgaacgcgc ccaccgcgct
     2881 ggtccatctc gatgcttcct gcaatcccgg caacacccgg tagcccgtca tgcgacgcga
     2941 ctgcgaaccg gctcacgttg ccggcgacca ggtagcgctg cggcgctgcg agctcgccgg
     3001 tccactcgac gtgcgcgtcg cgcacgctgc cgtgcggcgc gaaacgcacg atgtcgcgcc
     3061 gcaccgcctc gggaaggggc agatgcgacg caatggcggc gagcggctgc agctcgattt
     3121 cgccgaccgc aaggcggccg cctgcggccg ccgccggcgt cgcctcgtcg aggtcgagcg
     3181 cgagatccgt cggcccgaac cggctgccgt cgggcagcac gaacgcgagg ccgttcgcc
//
LOCUS       DASSVX010000371         3944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_482890, whole genome shotgun sequence.
ACCESSION   DASSVX010000371 DASSVX010000000
VERSION     DASSVX010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482890"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFO53_15730"
     CDS             complement(<1..206)
                     /locus_tag="VFO53_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877778.1"
                     /translation="MSRKRMTQPSGTTLVLEYESRVLADNPLGDPHVRKLAVWLPPGY
                     DSGTVRGRTSGRGRRFPVLFDLVG"
     gene            299..862
                     /locus_tag="VFO53_15735"
     CDS             299..862
                     /locus_tag="VFO53_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HET8877779.1"
                     /translation="MPRPEPAVADLPPITRSALFASFLKMGLLGFGGVLPWARRVIVD
                     ERRWLGDREFAELIGLCQVLPGPNVVNLSVIIGARTHGAVGSLIAVAGILLVPVGLML
                     VIAAFYATVAQDPLTRNAIAGASAAAAGLILGTAVRLLLHTRPPLRGLLTGGAAFVGV
                     GVLQWPLLWVILVLIVAGIGAEWRARR"
     gene            874..1407
                     /locus_tag="VFO53_15740"
     CDS             874..1407
                     /locus_tag="VFO53_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HET8877780.1"
                     /translation="MHADTQVLAEIATRFAALSLVAIGGINAILPEIHRVVVDLEGWM
                     TSAQFADLFALAQLAPGPNAMVVALVGWKVAGVTGALVATVAACGPSSVACYIAWHWA
                     DRLRESPTRALVQRALAPLAIGLILASGYTLARGADRSPGAFALTLLAAATLAWTRVN
                     PVWVLLVAGIAGLAGLA"
     gene            complement(1412..1903)
                     /locus_tag="VFO53_15745"
     CDS             complement(1412..1903)
                     /locus_tag="VFO53_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HET8877781.1"
                     /translation="MPEARTRKPPTTRPKPAYAVRHSPIHGRGVFATRTIRKGADIIE
                     YRGRRISVEEADELPDSDPDNPFHTFLFELNDGRVIDAGVRGNAARWINHSCQPNCVP
                     YENDEGRVIIEAKRTIRAGEELSYDYKLNVPGRRSARLLANYACRCGAPRCRGSMVDP
                     RKK"
     gene            2022..2528
                     /locus_tag="VFO53_15750"
     CDS             2022..2528
                     /locus_tag="VFO53_15750"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET8877782.1"
                     /translation="MRNTHMHIESARSRPARTAGSPAPADRLESLAILIGGLSKAWRE
                     ELDRRLRPLGLTRAQWQALLWLSRAGGTLVQRELSGCLEIGAPATVALVDRMERDGLV
                     ARSAVPDDRRRNAVVLTPKARRLLGTIESAAGKLRRDVMGGLTRDEVDTLCALLGKAK
                     LRIDALRA"
     gene            2525..3529
                     /locus_tag="VFO53_15755"
     CDS             2525..3529
                     /locus_tag="VFO53_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET8877783.1"
                     /translation="MTRLTFHLSPRSVLIVAGAIIVAIAGAWYWHESGQRQSTDDAYV
                     NARIVQVSSLVMGQVVDVPIHENQYVHTGDVLFELDRRPFEAALGEAEGRLRQAEQGT
                     RQDESDVLAAQADTAQQAAALTNAEANLRRTTQLVKQNFMSKQAEDDARARVNVAEAA
                     VAAAKARVAKARAALTTTPSGSTPAVRIAQAQVEKARLDLEHTVVRAPEEGWITRFDL
                     TAGTVVTPGNPLFAIVVGGSFWVDANFKETELAGVVPGRPATIVVDMYPDHTFHGKVE
                     SLAGGTGAAFSLLPAQNANGNWVKIAQRVPVRVSIADPDPAHPLRVGATATVRVSIAK
                     "
     gene            3612..>3944
                     /locus_tag="VFO53_15760"
     CDS             3612..>3944
                     /locus_tag="VFO53_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8877784.1"
                     /translation="MAATVVQVLDTTIVNVALPHMAGELSASTDQISWVLTSYIVAAS
                     IVMPMTGYLTDRLGRRRYLLISIAGFVAASALCGIATSLPEIVLFRLLQGVFGASLVP
                     LSQAIMVDS"
BASE COUNT          542 a         1343 c         1417 g          642 t
ORIGIN      
        1 aagccgacga ggtcgaacag caccggaaag cggcggccgc gcccgctcgt gcggccgcgc
       61 accgtgccgc tgtcgtagcc tggcggcagc cacactgcca gctttcgcac gtgcggatcg
      121 ccaagcggat tgtcggcgag caccctggac tcgtattcga gcaccagcgt ggtgcccgac
      181 ggttgcgtca ttctcttgcg gctcatgatg ggtcggcgtc gggaacgaat gggcgcgatt
      241 gtgacggccc gcgcgcaagc aggcaacgtt agaatcgacg gcttttacgt ggatcgacat
      301 gccacgaccc gagcccgcgg tcgccgacct tcccccgatt acccgcagcg cgctgttcgc
      361 gagcttcctc aagatgggat tgctgggctt cggcggcgtg ctgccgtggg cgcgtcgcgt
      421 gatcgtcgac gagcgccgct ggctcggcga ccgcgagttc gccgagctga tcgggctttg
      481 ccaggtgctg cccggtccga acgtcgtcaa cttgtcggtc atcatcggtg cacgaacgca
      541 cggcgcggtc ggctcgctga tcgcggtcgc cggcatcctg ctcgtgccgg tcggcctgat
      601 gctggtgatc gccgcgttct acgcgaccgt cgcgcaagac cctctgacgc gcaacgcgat
      661 cgccggcgcg tcggcggcgg ccgcggggct gatcctcggc accgcggtgc ggctcctgct
      721 gcacacgcgc ccgccattgc gcggcctgtt gaccggcggc gccgcgttcg tcggcgtggg
      781 cgtgctgcag tggccgctgc tgtgggtgat cctcgtgctg atcgttgccg gcatcggcgc
      841 cgagtggcgt gcgcgccgat gagcgcctga ccgatgcatg ccgacacgca ggtgctggcc
      901 gagatcgcga cgcggttcgc ggcgctgtcg ctcgtggcga tcggcggcat caacgcgatc
      961 ctgccggaga tccatcgcgt cgtcgtcgac ctcgaagggt ggatgaccag tgcgcagttc
     1021 gccgatctgt tcgcgctcgc gcagctcgcg cccggtccga acgcgatggt cgtagcgctc
     1081 gtcggctgga aggtggccgg cgtgaccggt gcgctggtcg cgacggtcgc cgcctgcggg
     1141 ccgtcgtcgg ttgcctgcta catcgcgtgg cactgggccg atcgcctgcg cgagtcgccg
     1201 acgcgtgcgc tggtgcagcg cgcgctggca ccgctcgcca tcggtctcat cctcgcgagc
     1261 ggctacacgc tggcgcgggg cgccgatcgc tcgcccggcg ccttcgcgct gacgctgctc
     1321 gccgcagcga cgcttgcgtg gacgcgcgtc aacccggtgt gggtgctgct ggtcgccggc
     1381 atcgccgggc tcgccggcct ggcgtagcga cttacttctt ccgcggatcg accatcgagc
     1441 cgcgacagcg cggcgcaccg caacggcacg cgtagttcgc aagaaggcgc gcactgcgcc
     1501 ggccgggaac gttcagcttg tagtcgtacg acagttcctc gccggcgcgg atggtccgct
     1561 tcgcctcgat gatgacgcgc ccctcgtcgt tctcgtacgg cacgcaattg ggctggcagc
     1621 tgtggttgat ccagcgcgcc gcgttgccgc gcacgcccgc gtcgatgacg cggccgtcgt
     1681 tgagctcgaa caggaacgtg tgaaacggat tgtcggggtc gctgtcggga agctcgtcgg
     1741 cctcttcgac cgagatccgc cggccgcggt actcgatgat gtcggcgccc ttgcgaatgg
     1801 tccgcgtcgc gaacacgccg cgtccatgaa tcggcgaatg gcgcaccgcg taagcgggtt
     1861 tcggacgcgt cgtcggtggc ttgcgggtac gggcttcggg catcggcggg gcgtcgggaa
     1921 ggttgcgcgg gacgttcggg agcgcgcgat tgtcgcatgc gcaggcggaa gcgggagtgc
     1981 gcgccttgaa aacggacggc gcgttccgca taatagttcg catgcgaaac actcacatgc
     2041 atatcgagtc ggcgcgaagc cggccggcgc gtaccgccgg ctcgccggcg ccggccgacc
     2101 ggctcgagtc gctcgccatc ctgatcggcg gcctgtcgaa ggcgtggcgc gaggagctcg
     2161 accggcggct gcgcccgctc ggcctgacgc gcgcgcagtg gcaggcgctg ctgtggctgt
     2221 cgcgcgccgg cggcacgctc gtccagcgcg agctctccgg gtgtctcgaa atcggggcgc
     2281 ccgcaaccgt cgccctcgtc gaccggatgg agcgcgacgg gctcgttgcg cgaagcgcgg
     2341 ttcccgacga ccggcgacgc aatgccgtgg ttctcacgcc gaaggcacgc cggctccttg
     2401 gcacgatcga atcggctgcc ggcaagctgc ggcgcgacgt gatgggcggc ctcacgcgtg
     2461 acgaagtcga cacgctgtgc gcgctgctcg gcaaggcgaa gctgcgcatc gacgcgctcc
     2521 gcgcatgacg cggctcacgt tccatctctc gccgcgatcg gtgctgatcg tcgccggcgc
     2581 gatcatcgtc gcgatcgccg gcgcgtggta ttggcacgag agcggccaac gccagtcgac
     2641 cgacgacgcc tatgtcaacg cgcgcatcgt gcaggtgtcg tcgctcgtca tggggcaggt
     2701 cgtcgacgtg cccattcacg agaaccagta cgtgcacacg ggcgacgtcc tgttcgagct
     2761 cgatcgccgg ccgttcgagg ccgccctcgg cgaggccgag ggacgactgc gtcaggcgga
     2821 gcagggcacg cggcaggatg aatcggacgt gctcgccgcg caggccgaca cggcgcagca
     2881 ggcggccgcg ctcaccaatg ccgaggcgaa cctgcggcgc acgacgcaac tcgtcaagca
     2941 gaacttcatg tcgaagcagg ccgaggacga cgcgcgggcg cgcgtcaatg tcgccgaggc
     3001 cgcggtcgcg gccgcgaagg cgcgcgtggc gaaggcgcgc gccgccctca cgacgacgcc
     3061 gagcgggtcg actcccgcgg tgcgtattgc gcaggcgcag gtcgagaagg cgcggctcga
     3121 cctcgagcac acggtcgtgc gcgcaccgga ggagggctgg atcacgcgct tcgacctcac
     3181 cgcgggcacg gtcgtgactc cgggcaaccc gctgttcgcg atcgtcgtgg gcggcagctt
     3241 ctgggtcgat gcgaacttca aggaaaccga gctcgccggc gtcgtgccgg gccggccggc
     3301 gacgatcgtc gtcgacatgt atcccgacca cacgttccac ggcaaggtgg agagtctcgc
     3361 cggcggcacc ggcgccgcgt tctcgctgtt gccggcgcag aacgccaacg gcaactgggt
     3421 caagatcgcg caacgcgtgc cggtcagggt gagcatcgcc gatcccgatc ccgcgcatcc
     3481 gcttcgggtc ggggccacgg ccaccgtccg cgtgtcgatc gcgaagtaag ccgtccatcg
     3541 tggccaacgt ctggaagtcg gctgcaggcg atgcgcaagg cggctcacgg ctgttcctga
     3601 cactcgccgt gatggcggcc accgtcgtgc aggtgctcga cacgacgatc gtcaacgtcg
     3661 cgctgccgca catggcgggc gagctttccg cgtcgaccga ccagatcagc tgggtgctga
     3721 cgagctacat cgtcgccgcc tcgatcgtga tgccgatgac cggctatctg accgaccgcc
     3781 tggggcgccg gcgctacctg ctcatcagca tcgcgggctt cgtcgccgcg tcggcgctgt
     3841 gcggcatcgc gacgtcgctg ccggagatcg tgctgtttcg tcttctgcaa ggcgtgttcg
     3901 gcgcctcgct cgtgccgctg tcgcaggcga tcatggtcga cagc
//
LOCUS       DASSVX010000372         9574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_1366276, whole genome shotgun sequence.
ACCESSION   DASSVX010000372 DASSVX010000000
VERSION     DASSVX010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366276"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VFO53_15765"
     CDS             <1..670
                     /locus_tag="VFO53_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HET8877785.1"
                     /translation="MSGLDLAQAVTCESRYEWTAGAWSLAGGYRATGAPKFHVVAYDY
                     GAKHNILRLLAQRGCRLTVVPAKTRASEVLALAPDGIFLSNGPGDPEPCDYAVAAIRE
                     LVERGMPTFGICLGHQLLGLASGARTVKMKFGHHGANHPVLDKDTGQVLITSQNHGFA
                     VDAASLPSNVRVTHVSLFDGSLQGIARTDRPAFSFQGHPEASPGPHDLGYLFDRFATM
                     MDRK"
     gene            673..3903
                     /gene="carB"
                     /locus_tag="VFO53_15770"
     CDS             673..3903
                     /gene="carB"
                     /locus_tag="VFO53_15770"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET8877786.1"
                     /translation="MPKRSDIESILIIGAGPIIIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADVTYIEPITWPMVASIIERERPDALLPTMGGQTALNCALDL
                     AREGILTKYDVELIGASKKAIDKAEDREKFKAAMTRIGLGSARSGIAHDLEQALDVQA
                     QIGFPAVIRPSFTLGGTGGGIAYNKDEFVDMCKRGLDLSPTRELLIEESLLGWKEFEM
                     EVVRDRKDNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQIMRDASIAVLREIG
                     VDTGGSNVQFAINPADGRMTVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LANDITGSEGGHFMPASFEPTIDYVVTKVPRFAFEKFRQADDRLTTQMKSVGEVMAIG
                     RTFQESLQKALRGLEVGVDGFNLKTVDPDTIADELAYPRAERIWYVADAFGIGMSLEE
                     LHAHTRIDPWFLAQIKEIVDTELAVEKRTLESIGRDDLRQLKRMGFSDRRLAHLTKTT
                     ETAVRTRRHALGIRPVYKRVDTCAAEFATRTAYLYSTYEDECEAEPTDRRKVMVLGGG
                     PNRIGQGIEFDYCCVHAAMALSEDGFETIMVNCNPETVSTDYDTSDRLYFEPLTLEDV
                     LEIVHVEKPWGVIVQFGGQTPLRLARDLEANGVPIIGTSPDMIDMAEDRERFQQMLRR
                     AGLRQPDNRTARNEDEALAAAEAIGYPLVVRPSYVLGGRAMEIVHEPRDLTRYMKEAV
                     RVSNDSPVLLDRFLNDATEVDVDAVCDGTEVIIGGIMEHIEQAGVHSGDSACSLPPFS
                     LSPALQDELRRQTIVMAKALRVVGLMNVQFAIQDDGASAPAVYVLEVNPRASRTVPYV
                     SKATGRPLAKIAARCMVGRTLAEQGVAGEVVPRYFCVKEAVFPFIKFPGVDTILGPEM
                     KSTGEVMGVGETFAEAFLKSQLGAGVRLPASGRVFLSIKDADKPAAIGVARSLHALGC
                     EIVATRGTAAALGAAGVAVRSVNKVKEGRPHIVDMLVNGEIAMVVNTVEEKRSSIQDS
                     YAIRRAALTGQVPTFTTIAGARAAAMGMAQMRDLVAYSLQALHRSL"
     gene            4082..5008
                     /locus_tag="VFO53_15775"
     CDS             4082..5008
                     /locus_tag="VFO53_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8877787.1"
                     /translation="MSTKASTRGAAMLVFTTLVWGAMFAVAKGALGAIDAFWLTAWRY
                     VPASLTMVALLWLVEGRRALSTDGAALRLWVYGSLGFAGFSILGYLGLARSRPEHAAI
                     IVALMPLFTSMANWLVRGRRPNGVTIAATLVALTGVVLVITRGHLQSAIDETLSADTL
                     VLAGVLCWIAYTMGAQTLPQFSTLRYTAHSMALGTLTIAGVTLAASVAGVAHAPSWPA
                     VAAHGGEIAYLSLIAGVLAVLAWNAGIAALGPANGVLFINLVPITAFAIGVAHGQRFG
                     ASEIAGATLVIGALVASSLAGRAPVARAPEFA"
     gene            5069..5545
                     /gene="greA"
                     /locus_tag="VFO53_15780"
     CDS             5069..5545
                     /gene="greA"
                     /locus_tag="VFO53_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009426112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET8877788.1"
                     /translation="MIKVPMTVEGAARLKAELHRLKTVERPAVIEALAEARSHGDLSE
                     NADYDAAKERQGFIEGRISEVESKLAHAQVINPADIEADGRVVFGSTVEVEDLGSGDR
                     NAYQIVGDDEADIKAGKISVNSPIARALIGKIEGDTAEVQAPGGLRSYEVRAVRYV"
     gene            complement(5536..6033)
                     /locus_tag="VFO53_15785"
     CDS             complement(5536..6033)
                     /locus_tag="VFO53_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877789.1"
                     /translation="MKHRFWLAVPLALAFTGTTVAQDIHKCVGDGAGVAYQNAPCAPG
                     QADAGLLRLPGYADPAERDGASAPAADASLAAPAEASPAEASFPALPAPAMSETQPVF
                     PFRTSVGLGMTDDQVLNVPGWGRPTTIVRSGSHGNWREVWTYDRGSDMRQLAFVGGRL
                     ASIQT"
     gene            complement(6153..6914)
                     /locus_tag="VFO53_15790"
     CDS             complement(6153..6914)
                     /locus_tag="VFO53_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8877790.1"
                     /translation="MKLNILSDLHLGQGALDVPDNDADVVILAGDISRPREAVAWARA
                     IDKPVLYVAGNHEFYGDSIDGTLDTLRNLCEGTSVRFLDDDVVVLDGVRFIGSTLWTD
                     FMLLGAARRGDAMREGQRFLRDFSRIRTRRHSDAIYTPADSVARFDVHRRFVAGELAK
                     PFAGPTVVITHHAPSPKSIHPRFRDSLLNACFISDLEHMMDGRRACLWVHGHTHDTFD
                     YAVNGTRVVCNPRGYAKNGVNENARFDPDLLVAVG"
     gene            complement(6931..8655)
                     /locus_tag="VFO53_15795"
     CDS             complement(6931..8655)
                     /locus_tag="VFO53_15795"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HET8877791.1"
                     /translation="MSRRKLRSEAWFARQDKMGFYYRSWLKNRGFPQDQFEGRPVIGI
                     CNTWSELTPCNSHFRTIAEHVRWGVLDAGGFPLEFPVMSLGETMLRPTAMLYRNLASM
                     DVEESIRANPLDGVVLLMGCDKTTPALLMGASSVDIPTIGVSGGPMLRGIHCGKAIGS
                     GTNVISMSEQLRSGQITLTEFHEAEADMHRSHGHCMTMGTASTMACMVEALGVGMPGN
                     AAIPAVDARRNLLAREAGRRIVELVKSDTVLSKILTREAFENAIRVNAGIGGSTNAVV
                     HLIAIARRIGVALSLDDWDRLGRGVHCLVDIMPSGRYLMEDLFEAGGLPVVLREMSKH
                     GLLHDGALTVNGRTIGENNRDAQCWKPDVVKTFDEPFKRDAGIAVLRGNLAPDGAIIK
                     PSAASAHLMQHTGPAVVFESIEDLHARINDDALDIDENSVMVLKYCGPKGYPGMAEVG
                     NMPLPPKLLKRGITDVVRISDARMSGTAYGTVVLHAAPESAAGGPLALVRNGDLVTLD
                     VAKRRLHLEVDDAELARRRAAWTPPPSHGERGYVKLYVDHVLQANDGVDLDFLVGRSG
                     SAVPRDNH"
     gene            complement(8672..>9574)
                     /locus_tag="VFO53_15800"
     CDS             complement(8672..>9574)
                     /locus_tag="VFO53_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HET8877792.1"
                     /translation="LVAVIGLFGLGELLISMEEGLRFDGVPARISPRAVLETIRSMPR
                     HWVALLRGSAIGCWMGITPGGPTAASFMSYGLAKRFSRRGRYFGSGEPEGVISPETAD
                     HAAGTSAMLPMLALGVPGSATSAVMLGGLMIWGLNPGPTLFIDRPDFVWGLIASMYVA
                     NIVAVVLVLSTVPLFAAILRIPFAIIGPMIVVVCFIGAYTVAGSKPDLLFAVVFGIAG
                     YAFKKLDYPIAPLVLAMVLGDKAEDAFRQSMIMSKGSLSIFWSNPLVSTLTAIGLLLL
                     CWPMIAAALRLMVPVRQPADAFED"
BASE COUNT         1599 a         3166 c         3283 g         1526 t
ORIGIN      
        1 gatgagcggg ctcgatctcg cgcaggcggt gacgtgtgaa tcgcgctacg agtggaccgc
       61 gggcgcgtgg tcgctcgccg gcggttaccg cgcgacgggt gcgccgaaat tccacgtcgt
      121 cgcctacgac tacggggcca agcacaacat cctccggctg cttgcgcagc gcggatgtcg
      181 cctcacggtg gttcccgcga agactcgcgc gagcgaggtg ctggcacttg cccccgatgg
      241 catcttcctg tcgaacgggc cgggcgatcc cgagccgtgc gattacgcgg tggccgccat
      301 ccgcgagctc gtcgagcgcg gcatgccgac gttcggcatc tgcctcggtc accagctcct
      361 cgggcttgct tccggtgcgc gcaccgtcaa gatgaaattc ggtcatcacg gcgcgaacca
      421 cccggtgctc gacaaggaca cggggcaggt gctgatcacg agccagaacc acggcttcgc
      481 tgtcgacgcg gcgtcgctgc cctcgaatgt gcgcgtgacc cacgtctcgc tgttcgacgg
      541 cagcctgcag ggcatcgccc gcaccgatcg tcccgcgttc agcttccagg gccacccgga
      601 ggcctcgccc ggtccgcacg acctcggcta cctgttcgac cgcttcgcga cgatgatgga
      661 caggaaatag cgatgccgaa gcggagcgac atcgagtcca tcctcatcat cggcgccggt
      721 ccgatcatca tcggccaggc gtgcgagttc gactattccg gggcgcaggc gtgcaaggcg
      781 ctgcgcgagg agggctaccg cgtcatcctc gtcaactcga atcccgcgac gatcatgacc
      841 gacccggaga tggcggatgt cacgtacatc gagccgatca cctggccgat ggtcgccagc
      901 atcatcgagc gcgagcggcc cgatgcgctg ctgccgacga tgggtggcca gacggcgttg
      961 aactgcgcgc tcgaccttgc gcgcgagggc attctcacga agtacgacgt cgagctgatc
     1021 ggggcgtcga agaaggcgat cgacaaggcc gaggaccgcg agaagttcaa ggcggcgatg
     1081 acgcgcatcg gcctcggctc cgcgcgctcc ggcattgcgc acgacctcga gcaggcgctc
     1141 gacgtgcagg cgcagatcgg ctttcccgcg gtgatccgtc cgtcgttcac gctcggcggc
     1201 accggcggcg gcatcgctta caacaaggac gagttcgtcg acatgtgcaa gcgcggcctc
     1261 gacctgtcgc cgacgcgcga gcttctgatc gaggagtcgc tgctcggctg gaaagagttc
     1321 gagatggagg tggtgcgcga ccgcaaggac aactgcatca tcgtttgctc gatcgagaac
     1381 ctcgatccga tgggggtgca taccggcgac tcgatcaccg tcgcgccggc gcagacgctg
     1441 acggacaagg aataccagat catgcgcgac gcctcgatcg cggtattgcg cgagatcggc
     1501 gtcgacaccg gcgggtcgaa cgtgcagttc gcgatcaacc ccgccgacgg ccgcatgacg
     1561 gtgatcgaga tgaacccgcg cgtgtcgcgc agctccgcgc tcgcgtcgaa ggccaccggc
     1621 ttcccgatcg cgaagatcgc cgcgaagctc gcggtcggct acacgctcga cgagctcgcc
     1681 aacgacatca ccggttcgga aggcggccat ttcatgccgg cgtcgttcga gccgacgatc
     1741 gactacgtgg tcaccaaggt gccgcgcttc gcgttcgaga agttcaggca ggcggacgat
     1801 cgtctcacca cccagatgaa gtcggttggc gaggtgatgg cgatcggccg cacgttccag
     1861 gaatcgctgc agaaggcgct gcgcggtctc gaggtcggcg tcgacggctt caacctgaag
     1921 accgtcgatc ccgacacgat cgccgacgag ctcgcgtacc cgcgcgccga gcgcatctgg
     1981 tacgtcgccg acgcgtttgg catcggcatg agcctcgagg agctgcacgc gcacacgagg
     2041 atcgatccgt ggttcctcgc gcagatcaag gagatcgtcg ataccgagct cgcggtcgag
     2101 aagcgcacgc tcgaatcgat cggcagggac gacctgcggc agctgaagcg gatgggcttt
     2161 tccgaccggc ggctcgcgca tctcacgaag acgaccgaga cggcggtgcg cacgcgccgg
     2221 catgcgctcg gcattcgacc cgtctacaag cgcgtcgata cctgcgcggc cgaattcgcg
     2281 acgcgcaccg cgtacctgta ctcgacgtac gaggacgagt gcgaggccga gccgacggat
     2341 cgcaggaagg tgatggtgct gggcggcggg ccgaaccgga tcgggcaggg catcgagttc
     2401 gactactgct gcgtgcacgc ggcgatggcg ctctcggagg acgggttcga gacgatcatg
     2461 gtcaactgca accccgaaac ggtgtcgacc gactacgaca cctccgaccg cctgtacttc
     2521 gagccgctca cgctcgagga cgtgctcgag atcgtgcatg tcgagaagcc gtggggcgtc
     2581 atcgtgcagt tcggcgggca gacgccgctc aggctcgcgc gcgacctcga ggcgaacggc
     2641 gtgccgatca tcggcacgtc acccgacatg atcgacatgg ccgaggaccg cgagcgcttc
     2701 cagcagatgc tgcgccgcgc cggtctgcgc cagccggaca atcgcaccgc acgcaacgag
     2761 gatgaggcgc tcgcggcggc ggaagcgatc ggctacccgc tcgtcgtgcg gccgtcgtac
     2821 gtgctcggcg gacgcgcgat ggaaatcgtc cacgagccgc gcgatcttac gcggtacatg
     2881 aaagaggccg tgcgggtctc caacgacagc ccggtgctgc tcgaccggtt ccttaacgat
     2941 gcgaccgagg tcgacgtcga tgcggtgtgc gatggcaccg aggtgatcat cggcggcatc
     3001 atggagcaca tcgagcaggc gggcgtccat tcgggcgatt ccgcgtgctc gctgccgccg
     3061 ttctcgctgt cgcccgcgtt gcaggacgag ttgcggcggc aaacgatcgt gatggcgaag
     3121 gcgctgcgcg tcgtcgggct catgaacgtg cagttcgcga tccaggacga tggggcgagc
     3181 gcgccggccg tctacgtgct cgaagtcaac ccgcgcgcgt cgcgcacggt gccgtatgta
     3241 tcgaaggcca ccgggcgccc gctcgcgaag atcgccgcac gctgcatggt gggacgcacg
     3301 cttgcggagc aaggtgtcgc cggcgaagtc gttccgcgct acttctgcgt gaaggaagcg
     3361 gtgttcccgt tcatcaagtt ccccggagtc gacacgatcc tcggaccgga aatgaaatcg
     3421 accggcgagg tcatgggcgt cggcgagaca ttcgccgaag cgtttctcaa gagccagctc
     3481 ggggcgggcg tgaggctgcc cgcctcgggg cgcgtcttcc tgagcatcaa ggacgccgac
     3541 aagccggcgg cgatcggcgt cgcacgaagc ctccacgcgc tcggctgcga aatcgtcgcg
     3601 acgcgcggca ccgccgcggc actcggcgca gcgggcgtgg cggtgcgctc ggtcaacaag
     3661 gtcaaggaag ggcgtccgca catcgtcgac atgctcgtca acggcgagat cgcgatggtc
     3721 gtcaatacgg tcgaggagaa gcgctcgtcg atccaggaca gctacgcgat ccgccgcgcc
     3781 gcgttgacgg gccaggtacc cacgttcacg acgatcgccg gcgcgcgtgc tgccgcgatg
     3841 ggcatggcgc agatgcgcga tctcgtcgcc tattcgctgc aggcgctgca ccgcagcctg
     3901 tgacggcgcg gccgcgcgca agcggcggcc tcggacccca ctcgcattac cagtgtgcat
     3961 gcagctcatg cacggaaatc gtttgcgcac accgggatgt gactggatca tgggcgcatg
     4021 ggcacgtgca gcttgattct gaggcatgcg tgcaagtcat tcgtgaacga gaggtatcgg
     4081 catgtcaacg aaagcatcga cacgcggcgc cgcgatgctc gtgttcacca cgctcgtgtg
     4141 gggggcgatg ttcgccgtcg cgaaaggcgc gttgggcgcg atcgacgcgt tctggctgac
     4201 cgcgtggcgc tacgtgccgg cgtcgctgac gatggttgca ttgctgtggc tcgtcgaggg
     4261 ccggcgcgcg ctgtcgaccg acggagcggc gctgcgcctg tgggtctacg ggtcgctcgg
     4321 cttcgccggc ttcagcatcc ttggctacct cgggctcgcg cgctcgcggc ccgagcatgc
     4381 cgcaatcatc gtcgcgctga tgccgctgtt cacgtcgatg gcgaactggc tcgttcgcgg
     4441 gcgccggccg aacggcgtta cgattgctgc gacgctcgtc gcactgaccg gcgtcgtgct
     4501 cgtcatcacc cggggacacc tgcagagcgc gatcgacgaa acgctctccg ccgatacgct
     4561 cgtgctggcg ggcgtgctct gctggatcgc ctacacgatg ggcgcgcaaa cgctgccgca
     4621 gttcagcacg cttcgctaca cggcgcacag catggcgctt ggcacgctga cgatcgccgg
     4681 cgtcacgctc gccgcgtcgg tggcgggcgt cgcgcatgcg ccgtcgtggc cggccgtcgc
     4741 ggcgcacggc ggcgagatcg cctatctgtc gctgatcgcc ggcgtgcttg cggtgctcgc
     4801 ctggaacgcg gggatcgcgg cgctgggccc cgcgaacggc gtgcttttca tcaatctggt
     4861 gccgatcacc gcgttcgcga tcggcgtcgc gcatggccag cgcttcggcg cgagcgaaat
     4921 cgccggcgcg accctcgtga tcggcgctct cgtggcgagc agcctcgcgg ggcgcgcgcc
     4981 ggtcgcacga gcgcccgaat tcgcgtaaac ttaaggcacg gccggaggcg gttcccgctt
     5041 ccggtcgttc gctttttggt gacgcgaaat gatcaaggta cccatgaccg tcgaaggtgc
     5101 ggcccggctc aaggccgaat tgcaccggtt gaaaaccgtc gagcggccgg cagtgatcga
     5161 ggcgctggcg gaggcgcgct cgcacggcga cctgtcggag aatgccgact acgacgccgc
     5221 gaaggagcgc cagggcttca tcgagggccg catctccgag gtcgagtcga agctcgccca
     5281 cgcgcaggtg atcaatcccg ccgacatcga ggccgacggc cgcgtcgtgt tcggctcgac
     5341 ggtggaggtc gaggacctcg gcagcggcga tcgcaacgcg tatcagatcg tcggcgacga
     5401 cgaggccgac atcaaggccg gcaagatctc ggtgaactcg ccgattgcgc gcgcgctgat
     5461 cggcaagatc gaaggcgata ccgccgaggt gcaggcgccg gggggattgc gcagctacga
     5521 ggtgcgtgcc gtccgctacg tttgaatgct ggccaggcga ccgcccacga acgcgagctg
     5581 gcgcatgtcg ctgccccgat cgtaggtcca cacttcgcgc cagttgccgt ggctgccgct
     5641 gcgcacgatt gttgtcggcc tgccccagcc cggaacgttg agcacctgat catcggtcat
     5701 cccgagcccg accgacgtgc ggaaggggaa gaccggctgg gtctccgaca tcgcgggtgc
     5761 gggcagcgcc gggaatgacg cttccgcggg cgaagcttcc gcgggcgcgg caagcgaggc
     5821 atcggcggca ggtgcgctcg cgccgtcgcg ttcggccgga tcggcgtagc cgggcagccg
     5881 aagcaggccg gcgtcggctt ggccgggtgc gcacggcgcg ttctgatagg cgacaccggc
     5941 gccgtcaccc acgcacttgt ggatatcctg cgcgacggtc gtccccgtga aggcaagcgc
     6001 gagtggaacc gccagccaga agcgatgttt cattgcgttc gtgagcctcg aattcgtcac
     6061 gaacgtaagg tgccctggcc gcgcgtgcat ggacatcggc cgcgtgcgcg cacgcgtgta
     6121 ggaatcgatg aaccgggcgc gccgggcgcg cgctagccga ccgcgacgag cagatcaggg
     6181 tcgaagcgcg cgttctcgtt cacgccgttt ttcgcataac cccgcggatt gcatacgacg
     6241 cgcgtgccat tgacggcgta gtcgaacgtg tcgtgcgtat gtccgtgcac ccacaggcag
     6301 gcgcgcctgc cgtccatcat gtgctcgaga tcggaaatga agcacgcgtt cagcagcgaa
     6361 tcgcggaatc gcggatggat gctcttcggc gacggcgcgt ggtgcgtgat gacgaccgtg
     6421 ggtccggcaa acggcttcgc gagctcaccg gcgacaaacc ggcgatgcac gtcgaaacgc
     6481 gcgaccgaat cggccggcgt atagatcgcg tcggaatgcc ggcgcgtgcg aatgcgcgaa
     6541 aaatcgcgca ggaaccgctg gccttcgcgc attgcgtccc cgcgccgcgc ggcacccagc
     6601 agcatgaaat cggtccacag cgtgctgccg atgaagcgga cgccgtcgag caccacgacg
     6661 tcgtcgtcga ggaagcggac gctggtgccc tcgcacaagt tgcgcagcgt atcgagcgtg
     6721 ccgtcgatgc tgtcgccgta gaactcgtgg ttgccggcca cgtagagcac cggcttgtcg
     6781 atcgcgcgtg cccacgcgac cgcttcgcgc ggtcgcgaga tgtcgccggc gaggatcacg
     6841 acgtcggcgt cgttgtccgg gacgtcgagc gctccctggc cgagatgcag gtcggacagg
     6901 atgttgagct tcattttgga agcccgctgc tcagtggttg tcgcgcggca ccgccgaacc
     6961 cgagcgaccg accaggaagt cgaggtcgac gccgtcgttc gcctgcagca cgtgatcgac
     7021 gtaaagcttc acgtagccgc gctcgccatg cgacggtggt ggcgtccacg cggcgcgcct
     7081 tcgcgcgagc tcggcgtcgt cgacttcgag atgcagccgc cgcttcgcga cgtcgagcgt
     7141 caccagatcg ccgtttcgca ccagcgcgag cggcccgccc gccgcggatt ccggtgccgc
     7201 gtgcagcacg acggtgccgt acgcggtgcc ggacatccgc gcatcggaga tgcgcacgac
     7261 gtcggtgatg ccgcgcttca gcagcttcgg cggcagcggc atgttgccga cctcggccat
     7321 ccccgggtac cccttcgggc cgcagtactt cagcaccatc accgagttct cgtcgatgtc
     7381 gagcgcgtcg tcgttgatgc gcgcatgcag gtcctcgatc gactcgaaca cgaccgcggg
     7441 gccggtgtgc tgcatcaggt gcgccgacgc ggccgacggc ttgatgatcg cgccgtcggg
     7501 cgcgagattg ccgcgcagga cggcgatgcc ggcatcgcgc ttgaacggct cgtcgaaggt
     7561 cttcacgaca tcgggcttcc agcactgcgc gtcgcggttg ttctcgccga tcgtgcgccc
     7621 gttcacggtg agcgcgccgt cgtgcagcag gccgtgcttc gacatctcgc gcagcacgac
     7681 gggcagcccg cccgcctcga acaggtcttc catcaggtat ctgcccgacg gcatgatgtc
     7741 cacgaggcag tgcacgccgc gtccaagccg atcccagtcg tcgagcgaca gcgcaacacc
     7801 gatgcgccgg gcgatcgcga tcaggtgcac cacggcgttc gtcgagccgc cgatgccggc
     7861 gttgacgcgg atcgcgttct cgaacgcttc gcgcgtgagg atcttcgaca gcacggtgtc
     7921 gctcttcacg agctcgacga tgcgcctgcc tgcttcgcgg gcgagcaggt tgcgccgcgc
     7981 atcgacggcc ggaatggccg cgttgcccgg catgccgaca ccgagtgctt cgaccatgca
     8041 cgccatcgtc gatgcggtgc ccatcgtcat gcaatggccg tgcgagcggt gcatgtcggc
     8101 ctcggcctcg tggaactcgg tgagcgtgat ctgccccgag cgcaattgct cgctcatcga
     8161 gatcacgttg gtgcccgagc cgatcgcctt gccgcagtgg atgccgcgca gcatcggtcc
     8221 gcccgacacg ccgatcgtcg ggatgtcgac gctcgacgcg cccatcagca gcgcgggcgt
     8281 ggtcttgtcg cagcccatca gcagcacgac gccgtcgagc ggattggcgc ggatcgattc
     8341 ctcgacgtcc atcgacgcga ggttgcgata gagcatcgcg gtcggccgca gcatcgtctc
     8401 gccgagcgac atcaccggga attcgagcgg aaatccgccg gcgtcgagga caccccagcg
     8461 cacgtgctcg gcgatggtgc ggaaatgcga gttgcacggc gtcagctcgg accacgtgtt
     8521 gcagatgccg atcaccggcc ggccctcgaa ctggtcctgc ggaaagccgc ggttcttgag
     8581 ccacgagcgg tagtagaagc ccatcttgtc ctggcgcgcg aaccaggcct cgctgcgcag
     8641 cttgcggcgg ctcatcttct tgatttctcc gtcaatcctc gaacgcgtcg gccggctggc
     8701 gcaccggcac catcaggcgc aatgcagccg cgatcatcgg ccagcacaat agcagcagcc
     8761 cgatcgcggt cagcgtcgag acgagcggat tggaccagaa gatcgacagc gatcccttcg
     8821 acatgatcat cgactggcgg aatgcgtcct cggccttgtc gccaagcacc atcgcgagca
     8881 cgagcggggc gatcggatag tcgagcttct tgaacgcgta acccgcgatg ccgaagacca
     8941 ccgcgaagag caggtcgggc ttgctgcccg ccaccgtgta ggcgccgatg aagcagacca
     9001 cgacgatcat cgggccgatg atcgcgaacg gaatgcgcag gatggccgcg aacagcggca
     9061 ccgtcgacag caccagcacg acggcgacga tgttcgcgac gtacatgctt gcgatcaggc
     9121 cccagacgaa gtcgggccgg tcgatgaaga gcgtcggtcc gggattgagc ccccagatca
     9181 tcagcccgcc gagcatcacc gccgacgtcg ccgaaccggg cacgccgagg gcgagcatcg
     9241 gcagcatcgc actcgtgccg gccgcgtggt ccgcggtttc cggcgagatg acgccctcgg
     9301 gctcgccgct gccgaaatac cggccgcggc gcgagaagcg cttcgcgagc ccgtagctca
     9361 tgaacgacgc ggcggtcggt ccgccgggcg tgatgcccat ccagcagccg atcgcgctgc
     9421 cgcgcagaag cgcaacccag tgccgcggca tcgagcggat cgtctcgagc acggctcgcg
     9481 gactgatgcg cgcgggcacg ccgtcgaagc gcagcccttc ttccatgctg atcagcagct
     9541 cgccgaggcc gaagaggccg atcaccgcga cgag
//
LOCUS       DASSVX010000373         8993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_766091, whole genome shotgun sequence.
ACCESSION   DASSVX010000373 DASSVX010000000
VERSION     DASSVX010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766091"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /gene="ligD"
                     /locus_tag="VFO53_15805"
     CDS             <1..967
                     /gene="ligD"
                     /locus_tag="VFO53_15805"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET8877793.1"
                     /translation="APGKCLNRRRVRAWYAACASSTTAMEHTHRYSPSPPNVAGVVIS
                     NAERPIVAAPGHTKLDVVRYHEALASWLLPQVAPRPIAVVKCMDGRFDDCFFQKHPLR
                     GRERDDDSPPFMRLADVADVVRAVQNGTYEFHTWGASFPRLERPDRITLDLDPDADLA
                     WPAVREAAEHVRTFLDRLELRWFVKTTGGKGLHFVLPITRRHTWLEARDFARAIAETL
                     ADDMPAVFTAKAAKDARAGRVYVDYLRNAEGATAVAAYSLRARAGLPVSMPIAWSDLE
                     RDIRGDCFNIDNAADAIARRRDDPWAGYDRARQTLSASMRRAVNA"
     gene            complement(999..1883)
                     /locus_tag="VFO53_15810"
     CDS             complement(999..1883)
                     /locus_tag="VFO53_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8877794.1"
                     /translation="MTASTELAVLFADVSGSTRLYETLGDERALATVGRCIALMREVC
                     TGHAGRVIKTIGDEVMAVFPLADEAAQAAAALQARIGAEPFAGGARVAIRVGFHHGPT
                     LAADGDVFGDSVNVAARLAGIAHGSQVITSGATAAMLSPWLRMRTRPLAALTVKGKAH
                     DLDVWELLWQDSADDLTTLATRVSVPRARLRLRHGEREIVLDEGQPALAFGRDAQNDL
                     VVADRLASRLHGRIERRRDRFVLIDHSSNGTFVTQAGEAEMPLRREEFVLRGRGHISF
                     GHAYATDPTETVEFDCDC"
     gene            2051..2938
                     /locus_tag="VFO53_15815"
     CDS             2051..2938
                     /locus_tag="VFO53_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8877795.1"
                     /translation="MNVVHAGTLLLLALWATAACAVERGIYTVVDGDVRVLRKTTWYR
                     LEAGARAESGDVIDAGERAQAQLELAHGGTLSIMGPALVLAAELPSDDAKPAAAELTL
                     LRGWFKASNAPKAPPLRLALPSSALRMAAGIAVVHCDAAHSEFFVESGSVTLTTPAAR
                     GREVAREAREGEFWHRSGDRAFEADDHPPPAFIDAMPRALRDKLPSLASRFAAAPGSL
                     TAGREVTFAEAEPWLTGTTRKAFARRFAPRLADPAFRAAAAAARPPVPEWDRTLHPER
                     YRPRESEDGSRPAASSANR"
     gene            2952..3821
                     /locus_tag="VFO53_15820"
     CDS             2952..3821
                     /locus_tag="VFO53_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HET8877796.1"
                     /translation="MKLIWKFNLVLLGIFVLGFAISGYVSYKVLQANAREEIVQNARL
                     MMEAALSSRTYTNTQVKPLLETQLKYAFLPQTVPAYAATEVFNEMRKKHPDYGYKEAT
                     LNPTNPRDRASDWEADIVNAFRASHDNIEMVGERDTPTGRALYLARPIQITSGACLVC
                     HSTIEVAPKTMIDQYGSANGFGWKMNEVIGAQVVSVPMSVPIKRADETFRTFMLSLAG
                     VFVVTFVLLNAMLHVMVIRRVTRLSAIADQVSLGNLDAGEFRTRSRDEIGVLTDALAR
                     MKVSLVQAMKMLE"
     gene            3828..5216
                     /locus_tag="VFO53_15825"
     CDS             3828..5216
                     /locus_tag="VFO53_15825"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET8877797.1"
                     /translation="MPDPVRIGKYEVERVLGKGAMGIVYKATDPHIERSVAIKTVRKD
                     LLDADLAAQFMARFRNEARAAGRLHHPNIIAIYEYGEDDSVAYIAMEYVDGMGLREYL
                     NRNARFELHQIAALVTQLLQALAFAHAQGVVHRDIKPANLILTAAGIVKVADFGIARI
                     DATTLTMTGMVMGTPTYMAPEQCRGMASDHRADLFSAGVVLYELLTGTRPFAGTMEAV
                     TYKICHEQPRRASEVCPFAVAPAIDAVIDKALAKNPADRFPDARAFELALRLACGTPA
                     GTHGGGETTLVNLADVRLSPTATLAFDDASLATIERQLVRFVGPMAKILVRQAALRAH
                     DARELYALLGVHIADPRERERFEAHAHAAEGSGSHATREGGAGRVPTNARGVATASAS
                     RTRASRPLEQAFIEQTTTRLAVYLGPIAKVVAKKAAQQAHDADEFVQLVAEHIGTQDR
                     SAFLREVATGDA"
     gene            5282..6247
                     /locus_tag="VFO53_15830"
     CDS             5282..6247
                     /locus_tag="VFO53_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET8877798.1"
                     /translation="MRYLRLVLAALLAAVAVNAAADDYPSRPVKIVVPFAAGGPADVY
                     ARFLAQRMQEAMGQPFVIENRPGGGSVVGTEIVAKSAPDGYTLLLMSNTHTVNESLMP
                     NKPFALMRDFTPVAPINYSDLVLVVNPDVKAKTLQELIALAKSEPGKLNYASSGPGTP
                     YHMAGELFKSMAGLDIVHVPYKESSGARTGVLGGQVEMMFDAVTVMNEHVKAGKVRAL
                     ATSGKLRSSVMPDVPTLSEAGVPGYEAVIWLGLIAPKNTPPAIVNRLNAEVTKIVSRP
                     ETRAEWAKQGATAMTMAPDAFGKFLADDIVKWERIVKLSGARADR"
     gene            6244..6963
                     /locus_tag="VFO53_15835"
     CDS             6244..6963
                     /locus_tag="VFO53_15835"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET8877799.1"
                     /translation="MTKRMPVLRVLSAGAAKGVVQTLAGPFEAASGARVEATFDSAGA
                     ISNAVTGGAGCDLVILPAAMLDTLAAQALVDGDSIAALGRVPTGIAVAAGDPLPDVHD
                     ADALKAALVAASALYCPDTMRSTAGIHFTGVLRTLGLYDASLAKLRAHANGAQAMAAL
                     AHAGAQRAIGCTQITEILYTPGVVLVAALPAPFELATIYGVAVASSTGAQAPARSFAA
                     RLTGAATQPLRRAGGFLDRAT"
     gene            complement(6989..7876)
                     /locus_tag="VFO53_15840"
     CDS             complement(6989..7876)
                     /locus_tag="VFO53_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HET8877800.1"
                     /translation="MERVEPLNPDTDFTRSADPRRRAARRRNLAWPIVAIIVIVAVIA
                     AVVLVWRATHAPEPVSAPAPQQPAAPAAAAPAETTPKHPIEAADATPPSATEALPAVA
                     DSDAALRDAMASLFAGIPLDRVFHLQEIVPRFVATIDNLPRQTVALSKMPVNPVEGAL
                     QTAPGDGRVLLRADNAERYATYMRVMEQVDTHKLVQTYVHYYPLFQKAYQDLGYPNGY
                     FNDRLVEVIDHLIATPDVPAPVALTQPKVLYEFADPALERLSAGQKMMIRMGPVNESR
                     AKAKLRDIRHALTGQALPR"
     gene            complement(8100..>8993)
                     /locus_tag="VFO53_15845"
     CDS             complement(8100..>8993)
                     /locus_tag="VFO53_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008865030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HET8877801.1"
                     /translation="PFDLERFLAGQQTPVFFGSAINNFGVQEILHALVGWAPAPLPRD
                     ATLRVVEPVEPSFSGFVFKIQANMDPRHRDRIAFFRVCSGRYQPAMKVRHLRIGREMK
                     LGNALMFMANERVSSEDAVAGDIIGIHNHGQLQIGDTLSEGESLAFRGIPYFAPELFR
                     VARPRDPFKAKPLSKGLQELGEEGAIQVFTSAHTNTVLLGAVGPLQFEIVAQRLATEY
                     NVDAIYETANISTARWLTYPDDATRRNFEREQAASLASDVDGNPVFLATNKYNLQVTV
                     ERWPKIGFHATREHGERLGHA"
BASE COUNT         1418 a         3045 c         3092 g         1438 t
ORIGIN      
        1 cgcgcccggc aagtgcctga atcgacgccg tgttcgcgcg tggtacgcgg cttgcgccag
       61 cagcacgacc gccatggaac atacccaccg gtattcgcct tcgccgccga acgttgcggg
      121 cgtcgtcatc agcaacgccg agcgcccgat cgtcgcagcg cccggacaca cgaagctcga
      181 cgtcgtccgc tatcacgaag cgctcgcttc gtggctgctg ccgcaagttg cgccgcggcc
      241 gatcgccgtc gtgaagtgca tggacgggcg cttcgacgac tgctttttcc agaagcaccc
      301 gctgcgcggc cgcgaacgtg acgatgattc gccgccgttc atgcgtcttg ccgatgtcgc
      361 cgacgtcgtg cgcgccgtgc agaacggcac ctacgagttt cacacctggg gtgcgagctt
      421 cccgcggctc gagcgtcccg accgcattac gctcgacctc gaccccgatg ccgatctcgc
      481 ctggccggcc gtgcgcgagg cagcagaaca cgtgcgcacg ttcctcgatc ggctcgagct
      541 gcgctggttc gtgaagacca ccggtggcaa gggcctgcac ttcgtgctgc cgatcacccg
      601 gcgccatacc tggctcgaag cacgcgactt cgcgcgcgcc attgccgaga cgctcgcgga
      661 cgacatgccg gcggtcttca cggcgaaggc cgcgaaggat gcgcgcgccg gacgcgtcta
      721 tgtcgactat ctgcgcaatg cggaaggtgc aacggcggtg gccgcgtact cgctgcgggc
      781 gcgcgccgga ttgccggtgt cgatgccgat cgcgtggtcc gacctcgagc gcgacattcg
      841 cggcgactgc ttcaatatcg acaacgcggc cgacgcgatc gcccggcgac gtgacgatcc
      901 gtgggccggt tacgatcgcg cgcgccagac gctgtcggcc tcgatgcgcc gcgccgtcaa
      961 cgcgtagctt gcaccacatc gccgccgccg gcgacgcttc agcaatcgca gtcgaactcc
     1021 acggtctcgg tcggatcggt cgcgtacgcg tggccgaagc tgatgtgtcc gcggccgcgc
     1081 aggacgaact cctcgcgccg cagcggcatc tccgcctccc ccgcctgcgt cacgaacgtg
     1141 ccgttcgagc tgtgatcgat gagcacgaaa cggtcgcggc gccgctcgat gcgcccatgc
     1201 agccgcgacg caagccggtc ggccaccacg aggtcgtttt gcgcgtcgcg cccgaacgcg
     1261 agcgcgggct ggccttcgtc gagcacgatc tcgcgctcgc catggcgcaa ccgcaagcgc
     1321 gcccgcggaa cgctgacgcg cgtggccagc gtggtcaggt cgtcggccga gtcctgccac
     1381 agcaattccc agacgtcgag gtcgtgcgcc ttgcccttga ccgtgagtgc cgcgagcggg
     1441 cgggtgcgca tgcgcagcca cggcgagagc atcgccgcag tggcgccgga agtgatcacc
     1501 tgcgatccgt gcgcgatgcc ggcgagccgc gccgcgacgt tgaccgaatc gccgaagacg
     1561 tcgccgtcgg cggcgagcgt cggaccatgg tggaagccga cgcggatggc gacccgcgcc
     1621 ccgccggcga agggttcggc gccgattcgt gcctgcagtg ccgcggccgc ctgtgcagcc
     1681 tcgtcggcga gcggaaacac cgccatcacc tcgtcgccga tcgtcttgat gacgcgcccc
     1741 gcatggccgg tgcagacttc gcgcatcagc gcgatgcagc gcccgacggt ggcgagcgca
     1801 cgctcgtcgc ccagcgtttc atagagtcgc gtgctgccgc tcacgtcggc gaagagcacg
     1861 gcgagttcgg tcgatgcagt catcgtcaat ccgggcgcgg gttggcgcgg gtggcagcgg
     1921 cagtataagg gcttgcgcat gcccggagcc gggcgcccgg ccgtgccgcc ccgagcggcg
     1981 acttttcgca ttgacgcagc gagaatggca agtctagtat cggtcgcgtc tcgagtccca
     2041 cctgtcaccg atgaacgtcg tccacgcggg tacgctgctt ctgctcgcgc tctgggccac
     2101 tgccgcgtgc gccgtcgagc gcggcatcta caccgtggtc gacggcgacg tgcgcgtgct
     2161 gcgcaagacg acgtggtatc gcctcgaagc gggcgcgcgc gccgagagcg gcgacgtgat
     2221 cgacgcgggc gagcgggcac aggcgcaact ggaacttgcg catggcggca cgttgagcat
     2281 catgggaccg gcgctcgttc ttgcagccga gcttccgtcc gacgacgcaa agccggcggc
     2341 agccgagttg acgctgttgc gcgggtggtt caaggcgtcg aatgcgccga aggcgccacc
     2401 gttgcggctc gcgctcccct cgtcggcgct gcggatggcc gcgggcattg ccgtcgtaca
     2461 ctgcgacgct gcgcacagtg aatttttcgt ggagagcgga agcgtgacgt tgaccacccc
     2521 cgccgcgcgg ggcagggaag tggcgcggga agcacgtgag ggtgagttct ggcatcgcag
     2581 cggcgatcgc gcgttcgagg ccgacgacca tccaccgccc gcgttcatcg atgcgatgcc
     2641 gcgcgcgctg cgcgacaagc tgccgagcct cgccagtcgc ttcgccgcgg ctccggggtc
     2701 gttgacggcc gggcgtgagg tcacgttcgc ggaagccgag ccgtggctca cggggacgac
     2761 ccgcaaggcg tttgccagac gctttgcgcc gcggctcgcc gaccccgcgt ttcgcgccgc
     2821 ggcggcggcg gcccgcccgc cggtgcccga atgggaccgc acgctgcatc ccgaacggta
     2881 ccggccgcga gaatccgagg acgggagccg ccccgcggct tcttcagcga atcgatgaca
     2941 agggcagtgc catgaagctg atctggaagt tcaacctcgt gctgctcggc attttcgtgc
     3001 tgggcttcgc gatctcgggc tacgtctcgt acaaggtgct gcaggcgaac gcgcgcgagg
     3061 agatcgtgca gaacgcgcgg ctcatgatgg aggcggcgct ttcgtcgcgc acctacacga
     3121 acacccaggt gaagccgctg ctcgagacgc agctcaagta tgcgttcctg ccgcagacgg
     3181 tgccggcgta tgccgccacc gaagtgttca acgaaatgcg caagaagcat cccgactacg
     3241 gctacaagga ggcgacgctc aacccgacca acccgcgcga tcgcgcaagc gactgggagg
     3301 ccgatatcgt caacgcgttc cgcgcctcgc acgacaacat cgagatggtt ggcgagcgcg
     3361 acacccccac cgggcgcgcg ctgtacctcg cgcgaccgat ccagatcacg agcggcgcct
     3421 gtctcgtctg tcacagcacg atcgaggtgg cgccgaaaac gatgatcgat cagtacggaa
     3481 gcgcgaacgg cttcggctgg aaaatgaacg aggtgatcgg cgcacaggtg gtgtcggtgc
     3541 cgatgtcggt tccgatcaag cgcgccgacg agacgttccg cacgttcatg ctgtcgcttg
     3601 ccggcgtgtt cgtcgtcacg ttcgtgctgc tgaacgcgat gctgcacgtg atggtgatcc
     3661 ggcgcgtgac gcgcctttcg gcgatcgccg accaggtgag cctcggcaac ctcgacgccg
     3721 gcgagttccg cacgcgcagc agggacgaga tcggcgtgct gaccgatgcg ctcgcccgca
     3781 tgaaggtgag tctcgtgcag gcgatgaaga tgttggagta atttgcgatg cctgatcccg
     3841 tccggatcgg caagtacgaa gtggagcgcg tcctcggcaa gggcgcgatg gggatcgtct
     3901 acaaggcgac cgatccgcac atcgagcgca gcgtcgccat caagacggtg cgcaaggacc
     3961 tcctcgacgc cgacctcgcc gcgcagttca tggcgcgctt tcgcaacgag gcgcgggcgg
     4021 cagggcggct gcaccatccg aacatcatcg cgatctacga atacggcgag gacgacagcg
     4081 tcgcgtacat cgcgatggag tacgtcgacg gcatggggct gcgcgagtac ctgaatcgca
     4141 acgcgcgctt cgagctgcac cagatcgccg cgctcgtcac gcagctttta caggcactgg
     4201 cgttcgcgca cgcgcagggc gtggtccacc gcgacatcaa gccggcaaac ctgatcctga
     4261 ccgccgccgg catcgtcaag gtggccgatt tcggcattgc acgaatcgat gcgaccacgc
     4321 tcacgatgac cgggatggtc atgggcacgc cgacctacat ggcgcccgag cagtgccggg
     4381 ggatggccag cgatcaccgt gccgatctgt tcagcgccgg cgtcgtcctc tacgagcttt
     4441 tgaccggcac gcggccgttc gccggaacga tggaggcggt cacctacaag atctgtcacg
     4501 agcagccgcg ccgcgcgtcg gaggtgtgcc cgttcgccgt ggcgccggcg atcgatgcgg
     4561 tgatcgacaa ggcgcttgcg aagaatcccg ccgatcgctt ccccgacgcg cgcgcattcg
     4621 agctcgcgct gcgcctcgcc tgcggtacgc cggcgggaac gcatggcggc ggcgaaacga
     4681 cgctggtcaa tctcgcggac gttcgcctgt cgccgactgc gactttggca ttcgacgacg
     4741 cgtcgctcgc gacgatcgag cgccagctgg tgcgcttcgt ggggccgatg gcgaagatcc
     4801 tggtgcgcca ggccgcgctt cgcgcacacg acgcgcgcga gctgtacgcg ctgctcggcg
     4861 tgcatatcgc cgacccgcgg gagcgcgagc gcttcgaggc gcacgcgcac gcggccgaag
     4921 gcagcggctc gcacgccacg cgcgaaggcg gcgcgggccg cgtgccgacg aacgcgcgcg
     4981 gtgtggccac cgcgtctgcg tcgcgcaccc gcgcgtcgcg gccgctcgag caggcgttca
     5041 tcgagcagac gaccacgcgg ctcgccgtgt atctcggacc catcgcgaag gtcgtcgcga
     5101 agaaggcggc gcagcaggcg cacgacgccg acgaattcgt tcagctcgtc gccgagcaca
     5161 tcggcacgca ggaccgcagc gcattcctgc gcgaggtcgc aaccggcgac gcctgaaaca
     5221 cggtgagacg ctgtttcggt agcattgcgc gtcgatcccc ccttccgccg aggaaccgcc
     5281 catgcgttac cttcgtctcg tcctggccgc cctgctcgcc gccgttgcgg tcaatgcggc
     5341 cgccgacgac tatccgtcgc gaccggtcaa gatcgtcgtg ccgttcgcgg ccggcggtcc
     5401 ggccgacgtc tacgcgcgtt tcctcgccca gcgcatgcag gaggccatgg gccagccgtt
     5461 cgtcatcgag aaccgtcccg ggggcggctc ggtcgtcggc accgaaatcg tcgcgaagag
     5521 cgcgcccgac ggctatacgc tgctgttgat gtcgaacacg cacacggtca acgagtcgct
     5581 gatgccgaac aagccgttcg cgctgatgcg cgatttcacg ccggtcgcgc cgatcaacta
     5641 ttccgatctc gtgctcgtcg tcaatcccga cgtgaaggcg aaaacgctgc aggagctgat
     5701 tgcgcttgcg aagtccgagc ccggcaagct caattacgcg tcgtcgggcc ccgggacgcc
     5761 ctatcacatg gccggcgagc tgttcaagtc gatggccggc ctcgacatcg ttcacgttcc
     5821 ctacaaggaa agctcgggcg cgcgcaccgg cgtgctgggc ggccaggtcg agatgatgtt
     5881 cgacgcggtg acggtgatga acgagcacgt gaaggcgggc aaggtgcgtg cgctcgccac
     5941 cagcggcaag ctgcgctcga gtgtgatgcc cgacgtgccg acgctttccg aggccggcgt
     6001 tccgggctac gaggccgtca tctggctcgg attgattgcg ccgaagaaca cgcctccggc
     6061 catcgtcaac cggctgaacg ccgaagtgac gaagatcgtc tcgcgtcccg agaccagggc
     6121 cgaatgggcg aagcagggcg ccaccgcgat gacgatggcg cccgatgcgt tcggcaagtt
     6181 ccttgctgac gatatagtca agtgggagcg catcgtgaag ctctccggcg cgcgcgccga
     6241 ccggtgacga agcgcatgcc ggtgctgcgc gttctgtccg cgggtgccgc caagggcgtc
     6301 gtgcagaccc tcgccgggcc gttcgaagcc gcatcgggcg cgcgcgtcga ggcgacattc
     6361 gactcggcgg gggcgatcag caacgcggtg accggcggcg ccggctgcga cctcgtgatc
     6421 ctcccggccg cgatgctcga cacgctcgca gcgcaagcgc tcgtcgacgg cgattcgatc
     6481 gcggcactcg gccgggtgcc gacgggaatc gcggtcgcgg cgggcgatcc gctgcccgac
     6541 gtgcacgatg ccgacgcatt gaaggcggcc cttgtcgcgg cgagcgcgct gtattgcccc
     6601 gacacgatgc gctcgaccgc cggcattcac ttcacgggcg ttttgcgcac gctgggtctc
     6661 tacgacgcgt cgctcgcgaa gctgcgcgcc catgccaatg gcgcgcaggc gatggccgcg
     6721 ctcgcacacg cgggagcgca gcgcgcgatc ggctgcaccc agatcaccga gatcctctat
     6781 acgcccggcg tcgtgctcgt cgcagcactg cccgcgccgt tcgagctcgc gacgatctac
     6841 ggcgtcgccg ttgcctcgag caccggcgcg caggcgccgg cacgctcgtt cgccgcgcgc
     6901 ctgaccggtg cggcaacgca gcccctgcgt cgcgcgggcg gatttctcga ccgggcgacg
     6961 tgacgctgcc gcaggtgtcc gcctcgcatc agcgcggcag cgcctgcccg gttagcgcat
     7021 ggcggatgtc gcgcaatttg gctttcgccc gcgattcatt gaccgggccc atccggatca
     7081 tcatcttctg cccggccgag agcctttcga gcgccgggtc ggcgaactcg tagagcacct
     7141 tcggctgcgt gagcgccact ggcgcgggca cgtcgggcgt cgcgatcagg tggtcgatca
     7201 cctcgaccag ccggtcgttg aagtagccat tgggatagcc gaggtcctgg taggccttct
     7261 ggaacagcgg atagtagtgc acgtacgtct gcacgagctt gtgcgtgtcg acctgctcca
     7321 tgacccgcat gtacgtcgcg tagcgttcgg cgttgtcggc gcgcagcagc acgcgtccgt
     7381 cgcctggcgc cgtctgcagc gcgccttcga ccgggttgac gggcattttc gacagcgcca
     7441 ccgtttgccg cggcaggttg tcgatcgtcg cgacgaagcg cgggacgatt tcctgcaaat
     7501 ggaacacgcg gtcgagcggg attccggcga agagcgacgc catcgcgtcg cgcagcgccg
     7561 cgtcgctgtc cgcgacggcg ggcagcgcct cggtggccga tggcggcgtc gcgtcggccg
     7621 cctcgatcgg atgcttcggc gtcgtttcgg ccggcgccgc tgcagcgggc gcggccggct
     7681 gctgcggcgc gggcgcgctt accggttcgg gagcgtgcgt ggcgcgccac acgagcacga
     7741 cggccgcgat gacggcgacg atgacgatga tcgcaacgat cggccacgcc agattgcgcc
     7801 gccgcgcggc acgccggcgc ggatcggccg aacgcgtgaa atcggtgtcg ggattgagag
     7861 gttcgacgcg ttccatccgg atctccaggg ctacaagggc tcgagcccgc ggcggctccc
     7921 gcggccaacg ggtttcgact tgaccgtgcg ccgatggttc ggcggcgggt aaatcggggt
     7981 cagagctgta attattccgg cgtcaatcca tgccgaccca tccgcgctcg tcgcggaata
     8041 attacggctc tgaccccgat ctcgattctg cgcggaataa ttacagctct gaccccgatt
     8101 caggcgtggc cgagccgctc cccgtgctcg cgcgtcgcat ggaagccgat cttcggccag
     8161 cgctcgaccg tcacctgcag gttgtacttg ttcgtcgcga ggaataccgg gttgccgtcg
     8221 acgtcgctcg cgagcgacgc ggcctgctcg cgctcgaaat tgcgccgggt cgcgtcgtcg
     8281 ggataggtaa gccagcgcgc cgtcgagatg ttcgccgtct cgtagatggc gtcgacgttg
     8341 tattcggtgg cgagccgctg cgcgacgatc tcgaactgca gcggcccgac ggcgccgagc
     8401 agcacggtgt tcgtgtgcgc cgacgtgaag acctggattg caccctcctc gccgagctcc
     8461 tgcagcccct tggacagcgg cttcgccttg aacgggtcgc gcggacgcgc gacgcggaag
     8521 agctccggcg cgaaatacgg aatgccgcgg aacgcgagcg attcgccttc cgaaagcgtg
     8581 tcgccgatct gcagctggcc gtggttgtgg atgccgatga tgtcgcccgc gaccgcatcc
     8641 tcgctcgata cgcgctcgtt ggccatgaac atgagcgcgt tgccgagctt catctcgcgc
     8701 ccgatgcgca ggtgccgcac cttcatcgcg ggctggtaac ggcccgagca gacgcggaag
     8761 aacgcgatgc gatcgcgatg gcgcgggtcc atgttcgcct ggatcttgaa cacgaagccg
     8821 ctgaacgatg gctcgaccgg ctcgacgacg cgaagcgtcg cgtcgcgcgg aagcggcgcc
     8881 ggcgcccagc cgacgagcgc gtgcaggatt tcctggacgc cgaaattgtt gatcgccgag
     8941 ccgaagaaga ccggcgtctg ttggccagcc aggaagcgct cgaggtcgaa cgg
//
LOCUS       DASSVX010000374         3943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_399943, whole genome shotgun sequence.
ACCESSION   DASSVX010000374 DASSVX010000000
VERSION     DASSVX010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399943"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..759)
                     /gene="mtgA"
                     /locus_tag="VFO53_15850"
     CDS             complement(55..759)
                     /gene="mtgA"
                     /locus_tag="VFO53_15850"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HET8877802.1"
                     /translation="MRRKGPFARALVLAAGLAVLAVVALHAYFATMIWWWRDHPPRET
                     AFMTERMDALRASNPRARLRYRFVPYERISTPLKRAMIAAEDAKFVDHEGFDWDGIAL
                     ALEKNEKRGHIVAGGSTITQQLAKNLFLSGSRSWLRKGEEAVITLMLEAMLDKRRIFE
                     LYLNVIEWGSGVFGAEAAAQRYFGESAARLSAEQSARLAAMAPNPRFYEHNPGAPGLN
                     RKIRIILARMPAADLP"
     gene            complement(764..1807)
                     /locus_tag="VFO53_15855"
     CDS             complement(764..1807)
                     /locus_tag="VFO53_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET8877803.1"
                     /translation="MPRRVAAPKPVQELAAEARPAAPSPARAPNANFVMAGALPPARK
                     PNLAMAGALPPARIPPEALATPRRVSTVAVAIALSVVLHAVALSLHFTVGSGRNHDPN
                     PPLEVALVNAKSLTKPTKADLLAQANLDGGGNTEQNRRAKTPLPALPDTQRRNVAVAA
                     SRQEVQENTTQEMMTRLRSPTAIPTPEPKPLAEPEPTESPTANDIMQKTLEAVRLEAK
                     IARDMDEYQKIPRRKHIGAQAREYRFARYVEDWRMKVERVGNTNYPAAARQKKLYGSL
                     ILTVFIRADGSVEKVEVNRKSGQRILDAAAVKIVEMAGPYAPFPDDIRRDTDILSITR
                     TWTFARGDEFSSE"
     gene            complement(1930..3801)
                     /locus_tag="VFO53_15860"
     CDS             complement(1930..3801)
                     /locus_tag="VFO53_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HET8877804.1"
                     /translation="MNVLFEDDGQLKAGTLLADHDASVQVEAVSGKRQKVKAAHVLLR
                     FADPSPAETMARAQKLVSELDPDFLWEVVPDDEFGFEELAREYYGATPAPAETAAVAL
                     ALARAPTHFYRKGRGRFRRAPPEALKAAVASLARKEREAREIAQWVAELQAHRLPAAL
                     AAKLDVLLYRADRNAPEWKALTLACEAARTSAVQLLRACGALASSHDYHYRRFIVEAF
                     PQGTQFPAWGSLPESPELPVAPVRAFSIDDASTSEIDDAFSVRDLGDERLEVGIHIAC
                     PALAITRGTPLDAIARQRLSTVYMPGRKVTMLPDEVVAQFTLAQEHSPPALSLYVEID
                     AEGTPLRHRTALERVPIAANLRHDALTDAFALPAAGDEPAWTAELRALWRLAVRLSGQ
                     RGKADVSRIDYSFDVDWDADGAGERGRVTITPRIRGNPLDKLVAELMIHVNHTWGRLL
                     ALARIPGLYRVQSGGKVKMSTRPGEHQGLGVGHYLWASSPLRRYSDLVNQRQLLAVVG
                     EVRAPYADNDAELVAALTDFEATYSQYAEMQDRMEHYWCLRWLLQDQVTRCNARVIRD
                     TLVRFDRLPLVMRLPDMPAQPADTPVRVAIGRIDLFDCTLECRYAGLAGDATTGESS"
BASE COUNT          613 a         1345 c         1355 g          630 t
ORIGIN      
        1 gcacgccgca ataattacag ctctgacccc aattaccaca gctctgaccc cgatctacgg
       61 caagtcggcc gccggcatcc gcgcgaggat gatgcgtatc ttgcggttca agcccggcgc
      121 gcccggattg tgttcgtaga aacgcggatt gggggccatc gctgcgagcc gcgccgactg
      181 ctcggccgac agccgcgcgg cggactcgcc gaaatagcgc tgcgcggccg cttcggcacc
      241 gaacacgccg ctgccccact cgatcacgtt gaggtagagc tcgaagatcc gccgcttgtc
      301 gagcatcgcc tccagcatca gcgtgatgac cgcctcctcg cccttgcgca gccagctgcg
      361 cgagcccgaa aggaacaggt tcttcgcgag ctgctgcgtg atcgtcgatc cgccggcgac
      421 gatgtgcccg cgcttctcgt tcttctcgag tgccagcgcg atcccgtccc agtcgaagcc
      481 ttcgtggtcg acgaacttcg cgtcctccgc ggcgatcatc gcgcgcttga gcggcgtcga
      541 aatgcgctcg tagggaacga accggtagcg cagccgcgcc cgcgggttgc tcgcccgaag
      601 cgcgtccatc cgctcggtca tgaaggccgt ttctcgcggc ggatgatcgc gccaccacca
      661 gatcatcgtg gcgaaatacg catgcagcgc gacgacggca agcacggcga ggcccgccgc
      721 gagcacgagc gctcgcgcga acggaccctt gcgcctcacc gcgtcactcg ctcgagaact
      781 cgtcgccccg ggcgaacgtc cacgtccgcg tgatgctgag gatgtcggtg tcgcgccgga
      841 tgtcatcggg aaacggcgcg tacggaccgg ccatctcgac gatcttcacc gcggcggcgt
      901 cgaggatgcg ctgacccgat ttgcggttga cctcgacctt ctcgacgctg ccgtcggcgc
      961 gaatgaagac ggtcaggatc aggctgccgt acagcttctt ctgccgtgcg gccgccgggt
     1021 agttcgtgtt gccgacgcgc tcgaccttca tgcgccagtc ttccacgtaa cgcgcgaagc
     1081 ggtactcgcg cgcctgcgct ccgatgtgct tgcgccgcgg aatcttctgg tactcgtcca
     1141 tgtcgcgggc aatcttcgcc tcgagccgca ccgcctcgag cgtcttctgc atgatgtcgt
     1201 tcgccgtcgg cgactcggtc ggctcgggct ccgccagcgg cttcggctcg ggcgtcggga
     1261 tcgcggtcgg cgaacgcaat cgggtcatca tctcctgcgt ggtgttctcc tgcacttcct
     1321 ggcgcgacgc ggcgacggcg acgttgcgcc gctgcgtatc cgggagcgca ggcagcggcg
     1381 tcttcgcgcg ccggttctgc tcggtgttgc cgccgccgtc gaggttcgcc tgcgcgagca
     1441 aatcggcctt cgtcggcttg gtcagcgact tcgcgttcac gagggccact tcgagcggcg
     1501 gattcgggtc gtggttgcgc ccggagccca ccgtgaagtg gagcgacagc gcgaccgcgt
     1561 gcagcacgac ggacagggcg atcgcaacgg caacggtcga cacacgccgc ggcgtggcga
     1621 gcgcctcggg cggaatgcgc gcgggcggca gtgcgccggc catggcgagg ttgggcttgc
     1681 gcgccggcgg cagcgcgccg gccatgacga aattcgcgtt cggcgcacgc gcgggcgacg
     1741 gcgcagcggg ccgcgcctcg gcggccagct cctgcacagg cttcggcgct gcgacgcgac
     1801 gtggcaagga cgcgatccgt tcgcggaact cttcggccgg ccgattgtag cgcggccatg
     1861 ccactgtcat gacaaatgaa ggcgtccgcc gatcggattg caccccccgc gtggcggcaa
     1921 tccaacgctt cagctgcttt cgccggtcgt tgcgtcgccg gcaaggcccg cgtagcggca
     1981 ctcgagcgtg caatcgaaca gatcgatccg cccgatcgcg acgcgcaccg gcgtgtcggc
     2041 gggctgcgcg ggcatgtcgg gcaggcgcat gacgagcggg agcctgtcga agcgcaccag
     2101 cgtgtcgcgg atcacgcgcg cgttgcatcg ggtgacctga tcctgcagga gccagcgcag
     2161 acaccagtaa tgctccatgc gatcctgcat ctcggcgtac tgcgaatacg tcgcctcgaa
     2221 atcggtgagt gcggcgacaa gctcggcatc gttgtccgcg tacggcgcgc gcacctcgcc
     2281 gacgaccgca agcaactgcc gctggttgac gagatcgctg tagcggcgaa gcggcgagct
     2341 cgcccacagg tagtgtccga cgcccaggcc ctgatgctcg ccgggacgcg tgctcatctt
     2401 caccttgccg cccgattgca cgcgatagag cccggggatg cgcgcaagcg cgagcagacg
     2461 cccccacgtg tggttcacgt gaatcatcag ctcggcgacg agcttgtcga gcggattgcc
     2521 gcgaatgcgc ggcgtaatcg tcacgcggcc acgctcgccc gcgccgtcgg cgtcccagtc
     2581 gacgtcgaag ctgtaatcga tgcgcgacac gtccgccttt ccgcgctgac cggaaaggcg
     2641 caccgcgagg cgccacagcg cgcgcagctc cgcggtccac gccggctcgt cgccggcggc
     2701 cgggagtgcg aaggcgtcgg tgagcgcgtc gtggcgaagg ttcgcggcga tcggcacacg
     2761 ctcgagcgcc gtgcgatgcc gcagcggcgt gccctcggcg tcgatctcga cgtagagcga
     2821 aagcgccggc gggctgtgct cctgcgcaag cgtgaactgc gccacgactt cgtccggcag
     2881 catcgtcacc ttgcgacccg gcatgtagac ggtggacagg cgctgccggg cgatggcgtc
     2941 gagcggcgta ccgcgcgtga tggcgagcgc gggacaggcg atgtggatgc cgacttcgag
     3001 tcgctcgtcg ccgaggtcgc gcaccgagaa cgcgtcgtcg atttcgctcg tcgatgcgtc
     3061 gtcgatggag aacgcgcgca cgggcgcgac cggcaactcg ggcgattcgg gcagcgagcc
     3121 ccacgcgggg aattgcgtgc cttggggaaa tgcttcgacg atgaagcggc ggtagtggta
     3181 atcgtgcgac gacgcaagcg cgccgcacgc acgaaggagc tgcacggcgc tcgttcgtgc
     3241 cgcttcgcag gcgagggtca gcgccttcca ctcgggcgcg ttccggtccg cccggtacaa
     3301 cagcacgtcg agcttcgccg caagtgctgc gggcagccgg tgtgcctgca gctcggcgac
     3361 ccattgcgcg atctcgcgcg cctcgcgctc cttgcgcgca agcgacgcca ccgccgcctt
     3421 cagcgcctcg ggcggcgccc tgcggaagcg gccgcgcccc ttgcgataga agtgggtggg
     3481 cgcacgggca agtgccagcg caacggcggc cgtttcggcg ggtgcgggcg tcgcgccgta
     3541 atactcgcgc gcgagctcct cgaagccgaa ctcgtcgtcc ggaaccactt cccacaggaa
     3601 atccggatct agctccgaga cgagcttttg tgcgcgcgcc atcgtctccg ccggtgacgg
     3661 atcggcgaag cgcagcagca cgtgggccgc cttgaccttc tgccgcttgc cggagaccgc
     3721 ctcgacctgc accgatgcat cgtgatcggc gaggagcgtg ccggccttca gctggccgtc
     3781 atcctcgaac agcacattca tgggaatcgg agaatcgccc gggcgcaaag caagctgcga
     3841 cgagccggcc gcggcgttga cgagagcgcg gattatagcg ggtaccattc agaggtcagg
     3901 agtcagagca cagaggacag cacccatgca gcgaagcgcg atg
//
LOCUS       DASSVX010000375         4426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U607
            k141_766428, whole genome shotgun sequence.
ACCESSION   DASSVX010000375 DASSVX010000000
VERSION     DASSVX010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968632
            Sequence Read Archive: SRR25158445
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:43:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,145
            Genes (coding)                    :: 3,127
            CDSs (with protein)               :: 3,127
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 2 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766428"
                     /isolate="SMAG_U607"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32194444 N 106.1388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFO53_15865"
     CDS             complement(<1..479)
                     /locus_tag="VFO53_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET8877805.1"
                     /translation="MIELDVNGRMHAIDADGDTPLLYVLRDHLAMNGAKFGCGLGQCG
                     ACTVIVDGKAVFSCVTPISVLGGRAIRTIEGLGTPDRPSPLQRAFIDEQAAQCGYCIA
                     GMIMRAQALLDANPSPSDAEIRRHMAPNLCRCGTHMRILRAIRRAAQRVPSAATPLR"
     gene            complement(476..1807)
                     /locus_tag="VFO53_15870"
     CDS             complement(476..1807)
                     /locus_tag="VFO53_15870"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET8877806.1"
                     /translation="MIALRAAHRLSMLALAAGTLLGCAGSPTTTAQTAADRTFDRAEV
                     AKGAQLSALGDCRVCHTVDGGSAFAGGRALPTPFGTLYSTNITPDVETGIGRWSRDDF
                     RRAMHEGIDRAGRHLYPAFPYDHFTRVTDDDVDALYAFVMTREPVTARAPRNRLVFPA
                     NLRPVLAAWNWLYLDARRFSADPGQSAEWNRGAYLVEGLAHCGACHTPRNIAGAEKRR
                     ERFRGGDAEGWHAPGILAASPAPVEWTAEALFRYLRNGFDAEHGLAAGPMGPVVDGLA
                     AVPEADVLAIVTYLTSLPGQERSKPAKDLVAAAEQREFDAVGRHGPDRANETSAGTAE
                     TEDAGEIIFAGACATCHYAGAGAPATRPVPLALATSVNASTPRNVLRVVVEGLHPAPG
                     ESGAIMPGFSGALTHAQIVAVVEFVRARFSDRPRWTDVNATLHRIEREQSK"
     gene            complement(1876..2619)
                     /locus_tag="VFO53_15875"
     CDS             complement(1876..2619)
                     /locus_tag="VFO53_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HET8877807.1"
                     /translation="MPRALIAEDEPLLRAQLRARLCDAWPELAEIIEAENGEQALALV
                     QERAPDVVFLDIRMPRKSGLDVARALAGRCHVVFVTAYDEYAIAAFDEGAVDYVLKPA
                     TAERMAKVVARVKARLALPPLDLAALLQRLAERDASGAPLKWIRASLGNVMQMIAVTD
                     VVYFRAEDKYTKVVTRDCEALIRTPIKDLYDELDQEAFWQIHRATIVNLDAIARVERD
                     FRDQPVITLKSRPERLTVSRTFAHRFKTM"
     gene            complement(2637..3734)
                     /locus_tag="VFO53_15880"
     CDS             complement(2637..3734)
                     /locus_tag="VFO53_15880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET8877808.1"
                     /translation="MATLPAPRLASSLRAGFSFSWTTLLLIIAIDTAIALVLWIDDPR
                     PFWQPLVTVQLYGMSIAYCINVAAPWDHDRPLRRLAVAALIGSLIGVVLVIVIKGYPL
                     SQVRERFGFFAMNVVAAFGNGLLISLIFYVKRREARAAAALHKAEAERHLLSKQAVEA
                     ELKLMQAQVEPHFLFNTLASVQYLIETDPKQASALLAHLIDYLRAALPQLRARSSTLA
                     REMSLVEAYLSILATRLGARLRFAIDLPAELRDHPFPPNLIISLVENAIKHGIEPSPD
                     GGSVTLSASRSGGSLIVSVTDTGCGASSETARSGQGVGLTNVRERLEALYGARARFSL
                     EPALPRGMRATLCLPDEADDGRARSAAGGAA"
     gene            complement(3840..>4426)
                     /locus_tag="VFO53_15885"
     CDS             complement(3840..>4426)
                     /locus_tag="VFO53_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET8877809.1"
                     /translation="DECYSEIHFDEAKPPLGALGAARALGRDGFPRLVVFGSLSKRSN
                     APGLRSGYVAGERALIGAFLQYRTYHGSAMSGAVAAASIAAWSDEAHVAHNRAEYAAK
                     FATLAPRLAEALPCAMPEAAFYLWASTPGDDVAFARRLLAEENVAVLPGSLLAREADG
                     VNPGAGRIRIALVADRAQCAEAVERIVACTQRQD"
BASE COUNT          710 a         1471 c         1549 g          696 t
ORIGIN      
        1 ctgcgcaacg gtgtcgcggc actcggaacg cgctgcgcgg cccgcctgat cgcacgcagg
       61 atgcgcatgt gcgtgccgca acggcaaaga ttgggtgcca tgtgccggcg gatttcggcg
      121 tcgctcggcg aaggattcgc gtcgagcagg gcctgcgcgc gcatgatcat gccggcgatg
      181 cagtagccgc attgcgctgc ctgttcgtcg atgaacgccc gctgcagcgg tgacggccga
      241 tcgggcgtgc cgagtccttc gatcgtgcgg atcgcccgtc cgccgagcac cgagatcggc
      301 gtcacgcacg aaaagaccgc ctttccgtcc acgatcaccg tgcaggcgcc gcattgcccg
      361 agcccgcagc cgaacttcgc gccgttcatc gccagatgat cgcgaagcac gtagagcagc
      421 ggcgtgtcgc catccgcgtc gatcgcatgc atgcgaccgt tgacatcgag ttcgatcatt
      481 tcgattgctc ccgctcgatc ctgtgcagtg tcgcgttgac gtcggtccag cgcggacgat
      541 cgctgaagcg cgcccgcacg aattccacca ccgcgacgat ctgcgcatgc gtcagcgcgc
      601 cgctgaagcc cggcatgatg gcacccgatt cgccgggtgc aggatgcagc ccttcgacga
      661 ccacgcgcaa cacgtttcgc ggcgtcgacg cgttcacgct ggttgcgagc gcaagcggta
      721 cgggccttgt cgccggcgcg cctgcgccgg cgtaatggca ggtggcgcag gccccggcga
      781 aaattatttc gccggcgtct tcggtttcgg cagtgccagc gctggtctcg ttggcccggt
      841 cggggccgtg acgtccaacg gcatcgaatt cgcgctgctc cgcggccgca acgaggtcct
      901 ttgccggctt gctgcgctcc tggcccggga ggctagtcag gtacgtgacg attgccagca
      961 cgtcggcctc gggcaccgcg gccaggccgt cgacgacggg ccccatcggt ccggcggcga
     1021 gtccatgctc ggcgtcgaag ccgttgcgca gataacggaa cagcgcctcg gcagtccatt
     1081 cgaccggcgc aggcgaggcg gcgagaatgc cgggtgcgtg ccagccttcg gcgtcgccgc
     1141 cccggaatcg ttcgcgcctt ttctcggcac cggcgatgtt gcgcggcgta tggcacgcac
     1201 cacagtgcgc gagaccttcg accagatacg cgccgcggtt ccattcggca ctctggcccg
     1261 ggtcggcgct gaagcggcgc gcatcgaggt agagccagtt ccaggcagcg aggactgggc
     1321 gcaggttggc gggaaagacg aggcggttgc gtggcgcacg cgccgtcacc ggctcgcgcg
     1381 tcatgacgaa cgcgtacagc gcgtcgacgt cgtcgtcggt gacgcgcgtg aaatggtcgt
     1441 acggaaatgc ggggtacaag tggcgtcccg cccggtcgat accctcgtgc atcgcgcggc
     1501 ggaaatcgtc gcggctccag cggccgatgc ccgtttcgac gtcgggcgtg atgttggtcg
     1561 aataaagggt gccgaacggc gtgggaagtg cccggccgcc ggcaaaggcg cttccgccgt
     1621 cgacggtgtg gcagacgcga cagtcgccga gcgccgacag ctgggcgccc ttcgcaacct
     1681 ctgcacggtc gaacgttcgg tccgctgccg tctgggccgt ggtggtcggc gacccggcgc
     1741 agccgagcaa ggtgccggcg gcgagggcca gcatcgacag gcggtgcgcc gcgcggaggg
     1801 cgatcatccg gctaagacac ggacgtgcgc gccgatgttc agtccgtctt cgctcgcgac
     1861 ggcaatcttc tgccgttaca tcgtcttgaa acggtgtgcg aaggtgcgcg agacggtgag
     1921 ccgttccggc cgcgacttga gcgtgatgac cggctggtcg cgaaagtcgc gctcgacgcg
     1981 cgcgatcgcg tcgaggttga cgatcgtcgc gcgatggatc tgccagaacg cttcctggtc
     2041 gagctcgtcg tagaggtcct tgatcggcgt tctgatcagc gcttcgcaat cgcgcgtgac
     2101 gaccttcgtg tacttgtcct cggcgcggaa atagacgacg tcggtcacgg cgatcatctg
     2161 catgacattc ccgagcgagg cgcgaatcca cttgagcggc gcgcccgagg cgtcgcgctc
     2221 ggcgagcctc tgcagcagtg cggcgagatc gagcggcggc aacgcgagcc gtgccttgac
     2281 ccgcgcgacc accttggcca tgcgctccgc cgttgccggt ttcagcacgt aatcgaccgc
     2341 accttcgtcg aacgcggcaa tcgcgtattc gtcgtatgcg gtgacgaaca cgacatgaca
     2401 gcggccggcg agcgcgcgcg ccacgtcgag ccccgatttg cgcggcatcc ggatgtcgag
     2461 gaacacgacg tcgggcgcgc gttcttgcac gagcgccagc gcctgctcgc cgttctcggc
     2521 ctcgatgatc tcggcgagct ccggccaggc atcgcaaaga cgtgcacgaa gctgcgcgcg
     2581 cagcaacggc tcgtcttcgg cgatcagcgc gcgcggcatc tcgacgtggc gtgcagctac
     2641 gccgctccgc cggcggccga acgagcccgg ccgtcgtctg cctcgtcggg caggcaaagc
     2701 gtcgcgcgca tgccgcgcgg caacgccggc tcgaggctga agcgcgcacg cgcgccataa
     2761 agtgcttcga gtcgctcgcg cacgttggtg aggcccacgc cctggccgct ccgggcggtc
     2821 tcgctcgatg cgccgcagcc cgtatccgtc acgctgacga tcagcgagcc gccgctgcgg
     2881 gatgccgaca gcgtgaccga tccgccgtcg ggcgacggct cgatgccatg cttgatcgcg
     2941 ttctccacga gggaaatgat gagattcggt ggaaacggat gatcgcgcag ttcagcggga
     3001 aggtcgatgg cgaagcgaag ccgcgccccg agccgcgtcg ccaggatgct gaggtacgcc
     3061 tcgaccagcg acatttcgcg ggcgagcgtc gacgaacgcg cgcgcagttg cggcagcgcg
     3121 gcgcgcaggt agtcgatcag atgcgcgagc agcgcgctgg cctgcttcgg gtcggtctcg
     3181 atcaggtatt gcacggaggc cagcgtgttg aagaggaagt gcggctccac ttgcgcctgc
     3241 atgagcttca gttcggcctc gaccgcctgc ttcgacagca gatgccgctc ggcttcggcc
     3301 ttgtgcaacg cagcggccgc gcgcgcctcg cgccgcttca cgtagaagat caggctgatc
     3361 aaaagcccgt tgccgaacgc ggcgacgacg ttcatcgcga agaagccgaa gcgctcgcgt
     3421 acctgcgaca gcgggtatcc cttgatgacg atcacgagca cgacgccgat cagcgacccg
     3481 atgagcgcgg cgaccgcgag ccggcgcagc ggcctgtcgt gatcccatgg cgcggccacg
     3541 tttatgcaat acgcgatcga catgccgtag agctgcacgg tgaccagcgg ctgccagaat
     3601 ggccgcggat cgtcgatcca cagcacgagc gcgatcgccg tgtcgatcgc aatgatcagc
     3661 aacagcgtcg tccagctgaa gctgaacccg gcgcgaagcg acgaggcgag acgcggagcg
     3721 ggaagggttg ccatgcccgg cattctagag accggcgggg ggcggggtgc gcggcgcgac
     3781 gaaacgcccg ctgcacggat gaagcgacgt cgcggacgac gggccgaacg acgcggtcgt
     3841 cagtcctgcc gctgcgtgca ggcgacgatg cgctcgaccg cttcggcgca ctgtgcgcgg
     3901 tcggccacga gcgcaatgcg gatgcgacca gcgccgggat tcacgccgtc ggcttcgcgc
     3961 gcaagcaggc ttccgggcag caccgcgacg ttttcctcgg ccagcagacg acgcgcgaac
     4021 gcgacgtcgt caccgggagt cgatgcccac aggtagaacg cggcttccgg catcgcgcac
     4081 ggcagcgctt cggcgagcct cggtgcgagc gttgcgaatt tcgccgcgta ttcggcgcgg
     4141 ttgtgcgcga cgtgcgcttc gtcgctccac gcggcgatgc tcgccgcggc gacggcgccc
     4201 gacatcgccg agccgtgata cgtgcggtac tggagaaagg cgccgatcag tgcgcgctcg
     4261 ccggccacgt aacccgagcg aagccccggc gcgttggatc gcttcgacag gctgccgaac
     4321 acgacgaggc gcgggaagcc atcgcggccg agtgcgcgcg cggcgccgag cgcgccgagc
     4381 ggcggcttcg cttcgtcgaa atggatctcc gaataacact cgtccg
//
